LOCUS       DAOOVC010000001        71341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_204_length_71341_cov_6.063084, whole genome shotgun sequence.
ACCESSION   DAOOVC010000001 DAOOVC010000000
VERSION     DAOOVC010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 71341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71341
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204_length_71341_cov_6.063084"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLM79_00005"
     CDS             <1..397
                     /locus_tag="PLM79_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV84716.1"
                     /translation="LDGRTVPKVSIAELARACGVGFVEEEDPYRFKPLVDLLKRAGKH
                     VRSSRGSVAVVIARRPCLMDRGRSDGWERFDIRVNEKCKGCLFCVKQFECPALQPRGQ
                     KEPIVLDRTLCRGCGVCVHVCPHGALEIA"
     gene            450..992
                     /locus_tag="PLM79_00010"
     CDS             450..992
                     /locus_tag="PLM79_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOV84717.1"
                     /translation="MSPIPRQQIIVSGVGGQGVLFATRILAEAALAANYHVLLSETHG
                     MAQRGGNVISHVKITGVEVPPFTSPLIRPGHADLFLGLHPQAFAVHGFYLKPDGRAFR
                     NEARSEDPDCLDAAEIAASLGSSISVNLVMLGFAASSRLLFCDAIQLEETVRSLSGPR
                     ADTALRLFRAGLDRGRALSL"
     gene            1020..1904
                     /locus_tag="PLM79_00015"
     CDS             1020..1904
                     /locus_tag="PLM79_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV84718.1"
                     /translation="MWWNLFPQFFVSGITSGSLYALIALGYCLIQNATGLVNFAQGEF
                     VMLGAMTAIALRQAVPSLPLPVVFALSMVLVALVGILLERGPLRRSRNRDILTLVMIT
                     VGASISLRGATMLLWGKNAHAFPPLGGSTPMLFWKAAVVPQTLWILFLSVVVLVLLYG
                     FYHHTLLGKAMRGAADNPVGATLIGISVGRLVALAFGMSGALGALAGILITPLTTMSY
                     DTGLMLGLKGFAGAILGGYGSVPGAVAGGLLLGILESFGAGFISSAYKDALAFLILLG
                     ILFLRPAGLFGSKTVRRL"
     gene            1901..3823
                     /locus_tag="PLM79_00020"
     CDS             1901..3823
                     /locus_tag="PLM79_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOV84719.1"
                     /translation="MKPGRTLSAAPPPGLSNEDGSRRGSVGTPFLPSDWRMLGALTAL
                     VLALPLIIRTDYTILLLNIIGLNSLVVLGLNLMIRATGQVSLGHAAFYGLGAYVSAVV
                     GVTFQWPILPSLLLALAVVTLMGFLLSLPTLRLEGHYLVMATLGFNIIVTIVFNQMED
                     ITGGPSGFPGIPKLSLGPWRIASDRDFYFFLWPLVILLLALSLNLMNSRTGRALEAIH
                     ERELTTRTLGIPTKRYKVAVFTLSTLYAGIGGFCYAHYVTFISPKTFDIFYSVQLVTM
                     AVVGGMGNLWGGLAGAALLTCLPELLQKFEDFYVLLYGLMLMGVLVFFPQGLLPFLTS
                     LPGRWKKSPRDPWEVPSSSRPAEVGAPGERNSPEGPDEDPGSAAPVHQGPPAMSVRDL
                     SVHFGGLQALYRVSLEVHPGRIVGLIGPNGAGKTTLLNVVSGLLKPDSGEVFLKERSL
                     RGLGPHEIAAAGLGRTFQSVQVYHRMNVLDNVLLGLHLRGRSGFWAGCWRTPGAGREE
                     RRLKEEALGLLESFGLGSREHWPVGRLSLLEQKLLELARARASDPSVLLLDEPAGGLN
                     PRESQCLVERISELRSQGLAMVLVEHDMNVVMGLADRVVVLQHGTRIAEGTPREVQQD
                     PRVIAAYLGNRKPGVR"
     gene            3851..4576
                     /locus_tag="PLM79_00025"
     CDS             3851..4576
                     /locus_tag="PLM79_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV84720.1"
                     /translation="MLRVAGLSARYGSIPVLRKVSFHVEAGEIVALVGSNGAGKSTLL
                     RTLSGLHPLTDGTILFRGRPIQSLSPATIVRLGLVQVPEARQLFPNLTVLENLELGGY
                     IHGRKHVARCLEGMFSLFPVLSRRKRQKAGTLSGGEQQMLSIARALMAKPALLLLDEP
                     SLGLAPLVIDEIYGAVLALSAQGVTILLVEQNAMGALGIARRAYVLENGRIVLAGDSE
                     ELMGHDDVRRAYLGRDYRHKWER"
     gene            4584..5921
                     /locus_tag="PLM79_00030"
     CDS             4584..5921
                     /locus_tag="PLM79_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV84721.1"
                     /translation="MMFPDPFGPISREEMRQWQLERLQTTLNRAYFQVDFYRKRMDAL
                     GLLPEDVAALEDLRTLPFTARRDLADHYPYGLFAVPLRSIVRLHTSTSTFQGKENPIV
                     LGFTRHDVEMWRSLAVRLFEQVEVTDRDIVQVAFNYSLFPGAFTFNQAVEALGATLAP
                     AATNSATIQLQVLQDFRCTVLATTAAFALHIADTMERTGTESSRLRLRLLLVGPEPLP
                     DPARARIESVLGVPVYGLYGVTEMVEPGLAGECTARDGLHLAEDQFLAEVVHPVTGEP
                     LPAGSEGELVLTTLTAEGYPLIRYRTGDVTVLREVPCPCGRKTARLSPVLRRTDNRVS
                     VRGIPFYPEDIELLLRRFDSGLEDFRLVISTVYGIGDQIEVLLTRTPAQDLPGGGRSQ
                     YLDLLRSQLRRALGLGVRVQMVEPDQLPREGLGYRTVFRSLTPVPDSPLPSPP"
     gene            6144..7442
                     /locus_tag="PLM79_00035"
     CDS             6144..7442
                     /locus_tag="PLM79_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV84722.1"
                     /translation="MRVSFMPQGSVREDLPRIQLQGLQWTVSHCYENSPFYRSLLDAA
                     GVKPDRVRSLEDLKRLPFTTAQDLKEGYPFPLCAVPHEKIVRIHASSGTTGKRKVLAY
                     TQKDVNDWADMFARCYEMAGLTRTDRVQIAVGYGLWTAGVGFQLGCERFGAMAIPVGP
                     ANVEIHCQMLQDMESTVFCSTASMALLMAEEIERRGLRKDIRLEKVIMGAERHSRRMR
                     ERIREITGVEHIFDIPGLTELYGPGTGLECVHHEGSHYWADYYILEILDPDTLEPVPP
                     GEIGEMVVTTLRKEAAPLIRYRTRDLTRLLPDPCPCGNPFPRHDHLLGRSDDMFIFRA
                     VNIYPGQIDHVLSQVPGMGSEYQVHLSRREDGRDVMLIKVERLWEDAPDRDRALAEMI
                     ASEIRRQILVRAQVEILEYGVLPRTERKSKRVFDHRPMQD"
     gene            7564..8757
                     /locus_tag="PLM79_00040"
     CDS             7564..8757
                     /locus_tag="PLM79_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV84723.1"
                     /translation="MKSRTRFIVPKVVRALALFAGLILAVQTVSAQEPIRIGAFFALS
                     GPAASIGTPTKLVAQMVVEKINKEGGIDQRPIQLVFGDTESDPTKALMEAKRLVEKEK
                     VVAIIGPTRTDTGMAVKPYIEDTAKVPILMTVGGDPVIAGGKFGPFHWTFKTPQRTSV
                     AVSKVYGYLKSKNLSKIALLTASDGFGKDGLLWLNQLVADYGLTITARESFNVTDTDM
                     TPQLVKIRESGAQALICWTIGPAGALVAKNVKQLALPIPLVQCHGQPDPKYIELAGDA
                     AEGSIMPSTKLMVADQLPESDPQKAVIREFLSLYEGTYQYQKQYPVNTHSGYAWDAVY
                     LLANAIRQAGTDPAKMRDAIEQTRGYVGISGIYNLTAQDHNGLGTDSLVMVRVEKGKW
                     VLLQD"
     gene            8799..10538
                     /gene="msbA"
                     /locus_tag="PLM79_00045"
     CDS             8799..10538
                     /gene="msbA"
                     /locus_tag="PLM79_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HOV84724.1"
                     /translation="MEIYRRLLNLVTPHWRRLALAMICMLGVAACTAASAYLIKPVLD
                     DIFVFKKMGMLKILPLLVLMLFVVKGVCAWGNAYWMSYVGQHIIAQLRQDLYSHMQTL
                     SLSFFDRMPTGVLMSRITNDVSQLQDAVTDSITGLLKDSFSILGLTVVVFYRDWQLAL
                     IAMAVLPFAFYPIVKFGRMLRRISRSSQQSMGEISVILHETISGNRIVKAFGMEEYEI
                     RRFGEANRRFLGYAMRSAVVKAMSSPIMEFLGGLGIVFIIWYGGYSVIQGVSTPGNFF
                     SFLAALLLLYEPVKRLSKMNNALQQGVAAAVRVYEILDTEPEIRDRPNASVLGPVSRG
                     ISLRNVSFHYGDQPVLKGIHLEIGVGEVVAIVGVSGAGKTTLVNLIPRFYDVTEGAVL
                     IDGTDIREVTLASLRSRIAMVTQQSILFNDTVRNNIAYGDVRKTDEEIIAAAKAANAY
                     DFILRLPEGFDTVIGEQGMRLSGGERQRICIARALLKDAPILILDEATSSLDSESELE
                     VQKALENLMAGRTTLVIAHRLSTVKNADRIIALVDGRIAEEGRHEELLRGDTEYRRLY
                     ELQFSQYGADSAG"
     gene            10653..10728
                     /locus_tag="PLM79_00050"
     tRNA            10653..10728
                     /locus_tag="PLM79_00050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10686..10688,aa:Gly,seq:ccc)
     gene            10956..11444
                     /gene="rimP"
                     /locus_tag="PLM79_00055"
     CDS             10956..11444
                     /gene="rimP"
                     /locus_tag="PLM79_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOV84725.1"
                     /translation="MQGSGEKGEGLPEQIRQLVEPVVESEGKELVELEYRRESHGWVL
                     RLFLDQEDGITVEDCARVSRVVGDLLDVADLIPNPYHLEVSSPGLNRPLRKVEHFRSA
                     LGKIIEIRTLAPLEGRKKFKGTLLEAAPESVRLDCDGRAYEIPLGLMDRARLCYFEST
                     EE"
     gene            11512..12852
                     /gene="nusA"
                     /locus_tag="PLM79_00060"
     CDS             11512..12852
                     /gene="nusA"
                     /locus_tag="PLM79_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOV84726.1"
                     /translation="MTGELKRLIDQVSREKGIDRQTLIHTLEEAIKSAIKKKHGSKLD
                     LDVTFNEEFGEIEAFLFKEVVEQVSDPEKQVSLEEALRLDPESEIGDELGIRMDTEAL
                     GRIAAQSAKQVIIQKMKDAEREVVFEEYKGRKGEIVNGIVQRVDKGGIVVNLGQAEAL
                     LPPKEQIPKEIYRQGDRIRAYVLEVRKISKGPQIILSRTHPHFVIQLFHAEVPEIAEG
                     IVSIISAAREPGSRAKIAVVSRDPDVDPVGACVGMKGTRVQNVVQELRGEKIDIVPWN
                     MDPAKFVVNGLAPAIISKVIIDQANRNMEVIVPDDQLSLAIGRRGQNVRLASRLTNWH
                     IDVKSESRYERQKQAGYQSLLEIPGLTEEIADRLYEAGITSLDDFVEASLTELEDLSR
                     LSQSDLEGMQAKAREKLIESREVEADLSEPSKPVEPSGPVEPEGPSEQEGELVE"
     gene            13327..16188
                     /gene="infB"
                     /locus_tag="PLM79_00065"
     CDS             13327..16188
                     /gene="infB"
                     /locus_tag="PLM79_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOV84727.1"
                     /translation="MRVHELAKELNMNNKDLIDRILKLGIVVKNHMSTLTESAVLKIR
                     QQFTEGRAETVEEKRIGRAIIRRRKKTTAEEELPPAEALGEGVSPVETEVVPEAAAAL
                     ELPEVPAEAAEVLEAEAPVEPGPEIAAIPAEVEMEARPVVVPPPPPPPLEPGPPAEEI
                     ATVELPAPREPEGPAVVEPPAAVPEEPQQEAAPVVPEVVAPEAPVTAQIHEVPLPRVE
                     DGEPAAEAEEEAEGAAKPKKAKKRRRKKVRKDEPARIIKLPEAVPEEPEDEVELLPIV
                     SRLHVKTEDREVKEAPRKKRTRPEEAEKEAAEFRKRAGVRKEVVEREDLYSKKELAAQ
                     ADRGRFRDRGRFVREPAEPEAAAPKPIKRRVRMDEAITIANLAKQMGVKAAEVIKKLL
                     VLGHPASINQALDYETAALLASEFDAEVEKVGFEEEEILQVQTDRPEELLLRPPVVTV
                     MGHVDHGKTSLLDAIRHTDVIAEEAGGITQHIGAYYVKLDHGEVVFLDTPGHEAFTAM
                     RARGAKVTDLVILVVAADDGVMQQTVEAIHHAKAAEVPIIVAINKVDKPNANVERVRR
                     ELADHGLLAEEWGGDVTMVEISAKKRLGIEELLEMVLLQAELMELKANPHKPARGRVI
                     EAKLDKGRGPVATILIQEGTLRAGDVYVCGTHSGRVRNMFNDRGVRLESAGPSVPVEV
                     LGFSGVPNAGDDFIVLADEKQAKLVAEHRLVKVREKELSRTTKVTLESLFEQIQEGEI
                     KELNVIVKTDVQGSLEAINESLSKLSTAAVKVNLIHSATGAITETDVMLASASNAIVI
                     GFNLRADAKVQELAEQERVDLRYYDVIYQLLNDIKDAMVGMLEPVYQEHVLGRAEVRQ
                     TFHVPRFGTIAGSYMLDGRVERGARVRLLRDQVVVYDGKISSLRRFKDDVKEVKAGFE
                     CGIGIENFNDIKVGDVLEFFELQEVKPTLEHEPEKRTGA"
     gene            16213..16506
                     /locus_tag="PLM79_00070"
     CDS             16213..16506
                     /locus_tag="PLM79_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOV84728.1"
                     /translation="MTVGVARITFRLHGNQSLKDKRKIVKGLVEKSRHRFNVSVAEVA
                     DQDLHQRATLGVAVVGSDHRVLNSLLDRIVAFMEAVGSADLVDHEIELIPLGD"
     gene            16519..16875
                     /gene="rbfA"
                     /locus_tag="PLM79_00075"
     CDS             16519..16875
                     /gene="rbfA"
                     /locus_tag="PLM79_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOV84729.1"
                     /translation="MTEFKRSDRVADLVKQEIADLLLRRVKDPRVSHVTVTGVKVSAD
                     LQHARVYYSVFPATSEVDKESAGAGLSKARGFIRQELARRLHMRVVPQLSFEYDTSLE
                     YGDRIERLIRELHKDE"
     gene            16868..17863
                     /locus_tag="PLM79_00080"
     CDS             16868..17863
                     /locus_tag="PLM79_00080"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOV84730.1"
                     /translation="MNEPSDKEIQRIAELIHRHRSFLVSTHVRPDGDAVGALLALVFM
                     LRRLGKRADPYCQDPSPACYEFLAGAETISTELPRPGVYEVAVLVDCGDLERVGPALA
                     PAIRTVPLTINIDHHSKNDTPFGTVSWVKPGASSTCEMLYDLSPALPVSLDDEIATHL
                     YTGLMTDTGSFRFSNTTRRVLEIATALVAAGADPARIAQEVYDSASPQGLKLLAQVLS
                     SVVFLSGDRLATMVLTQRMFQETGTTPEDTEGFINYIRSVKPVHLAMLFRQERNGMIH
                     VSMRSKGRVDVAALARRYGGGGHRHAAAFRVAGSLDRVRETFTDAALEYLDGIEG"
     gene            17915..18874
                     /gene="truB"
                     /locus_tag="PLM79_00085"
     CDS             17915..18874
                     /gene="truB"
                     /locus_tag="PLM79_00085"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOV84731.1"
                     /translation="MDRPASPPRPISTAQEVDGVLVVDKPEGLTSFAVVSILRRDLGV
                     GKVGHCGTLDPFATGVLVLCLNQATRIAELLATQDKRYEFRVRFGAETDTLDKTGRVV
                     STYAGPPVPRGRVEEALEGFAGGYLQEAPRFSAVHVRGKRLYHYARKGIFVEPPKREV
                     KIHELALRDFSWPEALLEVFCSKGTYVRQLASDIGRVLGCGAVVAQLRRLASGPFGTE
                     DCIRIEELKKGARDGSWQRAVLPMNRVLVQLESLVIREEEVLRALSCGHLDPSWEARM
                     KTRVAQSAGPVRLLTGTGRLAALWWPAHGAAGRSRLQVFRFEV"
     gene            18979..19248
                     /gene="rpsO"
                     /locus_tag="PLM79_00090"
     CDS             18979..19248
                     /gene="rpsO"
                     /locus_tag="PLM79_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOV84732.1"
                     /translation="MVMTPDRKKEIIENFKMHPSDTGSPEVQIALLSERINYLTDHFK
                     LHKKDHHSRRGLLKLVGQRRQLLNYLKRKNVDRYNTVIERLGLRR"
     gene            19321..21417
                     /gene="pnp"
                     /locus_tag="PLM79_00095"
     CDS             19321..21417
                     /gene="pnp"
                     /locus_tag="PLM79_00095"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOV84733.1"
                     /translation="MKYSVQVDVGGRPLTIECGHMARQASGSVLMRYGETVVLVSATK
                     EDRVREGIDFVPLMVDYQEMSYAVGRIPGGFFRREIGRPSEKETLTSRLIDRPIRPLF
                     PKGYSFETQVIATVLSVDLANEPDVIALSGASAALHISNIPFQGPIAAVRVGRVNGEL
                     IINPSLKQLDESDINLVVAGSRDAVVMVEGGADFVSEADMVEAIDFGHRALLPVLDLQ
                     EELRLMVQPEKQVFQGIQKDPALTEAVERTAAEEMLAVMTTADKQLRKERKKILRQRV
                     FDEILIEYPDREKEVEGILEEIEKRVVRRMMVEQKHRIDGRRFDEIRPITIEAGLLPR
                     THGSAMFRRGETQVLAVVTLGSSSDEQKIEALSGETFKSFMLHYNFPPYSVGEVRPLR
                     GPGRREIGHGALAERAVKAVLPRDGEFPYTIRVVSEVLESNGSSSMATVCGASLALMD
                     AGVPIREAVAGIAMGLIKEGEEFIILSDILGDEDHLGDMDFKVTGSENGITALQMDIK
                     ISGINRAILEQALEQARIGRLFILNKMKEILSSPRPELSPFAPRVVSIMINPEKIRDV
                     IGPGGKVIRSIVAETGAKIDIDDSGRVVVMSPDAQACEKAIDRIKRLTQEAEVGQTYM
                     ARVVRVTDFGAFAEILPNIEGLIHISQLEHKRVRKVSDVVNEGDEVLVKVIDIDKDGR
                     IRLSRKAVLEKPGETR"
     gene            21646..22920
                     /locus_tag="PLM79_00100"
     CDS             21646..22920
                     /locus_tag="PLM79_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOV84734.1"
                     /translation="MYQKTVLQNGIRVVTEKIPYVHSVSTGIWVCAGSRDEGQQERGI
                     SHFIEHMLFKGTQRRSALDIAKELDAVGGFANAFTSKEHVCFHAKVLDAHLPLVVDVL
                     TDIFLNSVFAPQEIEREQQVILQEIRMIEDTPDEYVHILFQERLWKDNPLGLPIYGSV
                     DTVEGIDRESILRYVSTTFDPKRIVIAAAGNLEHQRFIDLVAPSMERLRPSSNRIRRE
                     TPNSHHLVHVISRDLEQVHLCLGMKGTSQVDERRFACHLLNVILGSSMSSRLFQEIRE
                     RRGLAYSVYSFTNSHEDTGLMGVYAGVSPENVQETLDVILDQLALLTRDPIPPQELAA
                     SKEYLKGSMYLNAESTDARMNRLAKNEFLFGRQVSFEEVEQKIYEVSAEEVQAWFQTV
                     CRPGEFTAVLLGPVDPESIRLGPVVATNGSQV"
     gene            22933..23715
                     /locus_tag="PLM79_00105"
     CDS             22933..23715
                     /locus_tag="PLM79_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV84735.1"
                     /translation="MALCFQLIASGSKGNAILVCSPKTRILVDAGLSARELCKRLEKT
                     CTPANRLDALVLSHEHGDHVRGAGVLSRRFHVPVYLNQGTRENLPEQTGPMESMQIFQ
                     SGSPFSVGDLEIHPFSISHDAADPVGFVVEHNAVRLGICTDLGVATQLVRTRLRGCRG
                     IVLEANHDTDLLLNGPYPWELKQRIRGRHGHLSNSDAYELLDTLHHDRLKTVVFAHLS
                     EVNNHPDLVRTGSNRFFCRDSWRDVDFVIGKQHEISGEMELA"
     gene            23749..24687
                     /locus_tag="PLM79_00110"
     CDS             23749..24687
                     /locus_tag="PLM79_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV84736.1"
                     /translation="MESIPKCLLLQIDATEESLRPVRFLGRLYPDAAQMELILSYFQP
                     PLPPVYKQYPLPPSLVEKRKELLRSRETQTRSILDRARTVLKEAGFSEDKIQEHVQER
                     ESSVARHACSLADRKKVDAVLVQRHVCRDLEDFLKDDPTDALLHHCQVSPVWIADGEI
                     DPGHAAVCIQNEDASLRAVDHAAFMLAESPGQITILHVSRSVSRPIESGLTDYGSEFL
                     GWLNSPGGTPLRSFFDNSRRILQDAKIQEDRVRLAVHPVSWNVADTILGYCRERRVGV
                     TVLGHSGEQGILGFLRGSVTKRILSEFKNMAVWVTQ"
     gene            24875..25303
                     /locus_tag="PLM79_00115"
     CDS             24875..25303
                     /locus_tag="PLM79_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV84737.1"
                     /translation="MEKKKPVVLVAEDNLVNQQLAKRMLEVLGCEVDLAANGIEALNA
                     FETGSYDLVLMDCQMPEMDGLQASMAIREREKASDSRYPEGALVSRAVPIIAVTGQVS
                     DHDRDSCIAAGMNDFLSKPFRLHEVEAMLKKWLHDRRKHD"
     gene            25373..25936
                     /locus_tag="PLM79_00120"
     CDS             25373..25936
                     /locus_tag="PLM79_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOV84738.1"
                     /translation="MRPHSYGRFWILGAVTLWLLSHPVPATPLEDSPQPPAPTEEEWM
                     LFQEIPSVYGASRYEQKVTEAPSSVSIVTSADIKHYGYRNLGEILESVPGLYTTYDRN
                     YSYLGMRGFARAGDYNTRVLLLVDGHRLNDAVYDQAPIGTDFPVDVDLIDRVEIIRGP
                     SSSIYGTNAFLGVINVITKRGRDLRGS"
     gene            26165..27391
                     /locus_tag="PLM79_00125"
     CDS             26165..27391
                     /locus_tag="PLM79_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV84739.1"
                     /translation="MGVFHSREKGIPTAPWDVIFNDPSTRTTDHRAFLDLKYDRELGD
                     RLDLSAHLYYDHYRYDGDYAYWQDPDRRWNRVLQKDYARGEMAGADVQLTKRWLENHK
                     LLLGSEYRGSFRQDQGTYDNHPYRLLMKERRDAYNWAVYLQDEYRIFSNLLLNAGVRY
                     DQYETFGGSTNPRLALIFNPIEKSALKLIYGQAFRAPNSYELFYTDRINQKANPDLDP
                     ENMTTYELVWEQRLTDRLRLSATGYYYEIEDLISLTRMPNGFLMFKNVENVQAKGLEF
                     ELDSRWPGGIEGRVSYCLQRTEDERSGNVLPNSPKQMVKLNVLCPLIQHKLTAGTELQ
                     YLSPRRTLRDNKTQDALIANLTFHAPNVVKGLDVSAGVYNLLDQDYGDPGSAEHLQDI
                     IQQNGINFRLKITYSF"
     gene            27520..28464
                     /locus_tag="PLM79_00130"
     CDS             27520..28464
                     /locus_tag="PLM79_00130"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV84740.1"
                     /translation="MRRVARGLLGTTAALLFLFSMAEATSEKTGTEVAVLLSHRVQPY
                     EQALAGFQQHLRERETSGPIQVHHLGEEVGRDAQAADSIAREPPRVLLTVGSPAARLA
                     AERLSHIPMVAGMILSPEDVKAHPNATAVVLDIPLEVQLAWLTRVLPGCRNVGVLYHT
                     ESNERKVETAGVLADRMGLRLYARKVQSPSEIPDALGFLSKRADVLWGLPDSLVVTPQ
                     TAKPLLLFSFRNNIPFVGLSSSWTKAGSLYSLDCSYPDIGVQCAEVVLKVLRGTPPRS
                     IPRVHPRKVLLSLNLKTAQHMRLHIPEPVVRSSEQVFE"
     gene            28479..31370
                     /locus_tag="PLM79_00135"
     CDS             28479..31370
                     /locus_tag="PLM79_00135"
                     /inference="COORDINATES: protein motif:HMM:NF021499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV84741.1"
                     /translation="MLGRWRIGLVGKFNLLTISLILITSLGIASFVIRQTSENQHRAL
                     IQHGITTADILSQNSEYALYTEDPELMKQILESVSAIENISYAALLDARRQVLLETRF
                     THSVQVPTHLRQVHSGNETLFEETPEDKSGRRFIDIVAPVLVHPELDLDVMQDKSRER
                     EGPKKLGYVQLGLDMERLHRRSREFLLSTLFFTTLIGLAGIFVSIMFTRRIAGPVRAL
                     AAVTREISEGKLRKPIEIDARDEIKDLTDSFNVMLERLRESREQVEDYQRMLEEKVRE
                     RTIQALRADEASRAKSQFLANMSHEIRTPMNGVLGLAEILLQGDLTDRQRNLAETIHH
                     SGMILLRILDDILDFSKIEAGRLELDHAPFDVAEAVEDVAELFAEQAQKKNLELVVDI
                     AADPPPIVHGDPARLRQILTNLVSNAIKFTEKGEVLVRLGWVESRDDSVRMGFEIRDT
                     GIGISEQDQPRIFEVFTQADSSSTRRHGGTGLGLCISRQLCEIMGGKIRVRSEVGKGS
                     TFFFDVVFGTPGTPVDPRTGLLPGRPDPPRVLIVDDNETCRRVLLNYCRGWNLSAEAA
                     SHGMEALRLLRNAAARGKGYDFLLLDADLGEKDGIELAGAVTRLLGENSPRVLLATTL
                     GQQERLEEAYEAGVSVCLRKPVRKSRLAEKIAGLWLRGAPGEAPASREETREPRREIL
                     AGARVLLAEDNEINQEVARLMLEGLGCRVHTVDNGIQAVDAVGRNPYDLVFMDCQMPE
                     LDGYEATRRIRDWEKERHSSGSGKPFSRIPIVALTAHAMAGDRDQCLAAGMNDYLSKP
                     FTLDQLRRVLLRWYPAGGTEPNRDEPPMEFSAAPHNPADRSSPLDPGALEAIRSLHRE
                     GEPDILPDLIEIFKRESAQFLETIRQAMDREDPETVRKIAHSWKSSSANLGATHLAKL
                     CMEMEQLGRSGHLEPAARILTKVEAELRLVFEALDSEIPKDESTRPSQG"
     gene            31378..32352
                     /gene="nudC"
                     /locus_tag="PLM79_00140"
     CDS             31378..32352
                     /gene="nudC"
                     /locus_tag="PLM79_00140"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HOV84742.1"
                     /translation="MSSFLSRIPLRDFHRSASRRSDPELLQRNPAPKEILYLPLWDDL
                     NLFSPLGDAQAVVLGKEAVGGLGGDVSAVSIYLGETPRSVFFALCLSAEAGFNPEALS
                     RYGSFLDLRKALPVLDSEQFSLLAFAKSLACWQGRALFCGACGAGTRAGHGGHVRTCS
                     NETCARQYFPRTDPAIIVLVLRGERCLLGRQSSWLPGRYSVLAGFVEPGEALEEAVIR
                     EVEEETGVRVRDVRYHSSQPWPFPSSLMIGFTARALDDRIDGVPGELEDARWFSRKEI
                     AGSLRDGSLVLPPRASIANRLITEWYDAGESLTLNDLLESLPHRQAFR"
     gene            32383..33381
                     /locus_tag="PLM79_00145"
     CDS             32383..33381
                     /locus_tag="PLM79_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV84743.1"
                     /translation="MQGYLLRRLIQIIPTLLGITFITFLIIQLAPGNPALLKLEMTGQ
                     GQMSDSAVSQQIIEQTRQLYGLDKPLPVQYLLWVKRVFTLDFGTSYKDHRDVWDKILE
                     RLPVTLQLNLASIFLVYLIAIPCGVYSATHAGSLTDRFLTLLFFLLYSLPGFWVAVLL
                     IMLLGGGDFWDWFPVYGISSIGAESWSFSRWLQDRLWHLVLPVVCQTYGGLAYLSRLT
                     RAEMLEVIREDYVRTARAKGLRERVVIFKHAFRNALLPIVTLLAFLLPSMFGGSVIIE
                     SIFSIPGMGQLGFEAVLSRDYPVIMAITTISALLTLAGLLISDLLYAVMDPRIKLE"
     gene            33428..34450
                     /locus_tag="PLM79_00150"
     CDS             33428..34450
                     /locus_tag="PLM79_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV84744.1"
                     /translation="MKLPRSYRQMVWHHFKKKRLSVWGLRVTLLLFAVALFAPLLAND
                     RPIVFYREGRWSWPVFSGQDKVGSLTWKEIRKGADFGTHPGGSFEKNWVAVWPPVPYS
                     PTEYDLVESLAPPGRDHWFGTDDSGRDVLSRMIHGARISLSVGFVAVGIALVIGIFLG
                     ALAGYFGGWTDHLISRMIEILLTIPTFFLIIAIIAFLPPSIYNIMIVIGLTGWSGVAR
                     FVRAEFLKIKQLDFIQASRSLGASHLRIIFVHMLPNSMAPVLVSAVFGIAGAILTESS
                     LSFLGFGVPPPTPSWGDILSQSRDYIEFAWWLTVFPGLAIFLSITAYNLVGEGLRDAM
                     DPKLFR"
     gene            34566..35936
                     /gene="xseA"
                     /locus_tag="PLM79_00155"
     CDS             34566..35936
                     /gene="xseA"
                     /locus_tag="PLM79_00155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOV84745.1"
                     /translation="MRQQALAETHVYTVSELNAEIRDLLESRYPFVWVTGEISNFRVP
                     SSGHFYFTLKDEQSQIRGVFFRSQNRYLRFVPESGIQVVCRGRLGVYEPRGEYQFIVD
                     VMEPRGLGALQLAFEQLKKKLGSEGLFDASRKKPMPLCPRRVAVVTSSTGAAVRDILK
                     VLERSPYPLEITILPVRVQGQGAAEEIAGAIRKAGELRNEFGWDVLLVGRGGGSIEDL
                     WAFNEEAAARALASCPIPTISAVGHEIDYTISDFVADLRVPTPTAGAQWVLRRMEEFQ
                     RELAHGTDRMVEVLRRKIEGSRQAVEMLEKRLVSPRRRLEDWKLFLDDRLERMHLAMA
                     RRMERQQTSLAHLVDRLRSRHPSTTIQQSRIELCRLQREMAFQLSKNLEARKYRLQEY
                     ASRLDSLSPLAVLQRGYAIGYRLEDMAVLRNSGQVCPGDEIGLRLSQGALKCTVQGEW
                     ETTPLS"
     gene            35947..36192
                     /gene="xseB"
                     /locus_tag="PLM79_00160"
     CDS             35947..36192
                     /gene="xseB"
                     /locus_tag="PLM79_00160"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOV84746.1"
                     /translation="MSRKKSEPFEDALKKLQAVVEKLERGDLPLEEAVESFTEGIRLV
                     QFCHRKLEEAEKKVQVLMADPEGGVSALPFEAESSQD"
     gene            36287..37225
                     /locus_tag="PLM79_00165"
     CDS             36287..37225
                     /locus_tag="PLM79_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOV84747.1"
                     /translation="MQPFDLKKYLTERKEWVETALEQYFPPSSRLEKRVVEAARYSLF
                     AGGKRLRPILCLAAAEAVSGTRQCAASAEMLGSSFQAVMPVACAMEMIHTYSLIHDDL
                     PAMDNDDFRRGRPTNHKVYGEAVAILAGDALLTEAFGILARLRTNGMFPAERILRVVD
                     IIAEAAGARGMIGGQTVDLESENREVDMDTVVYMHRCKTGALITASLEAGAVLVGGEE
                     DRVADLTRYGHHLGMAFQITDDLLDVEGDARLMGKNPGSDAAKNKKTYPALLGIEASR
                     ESAKHHVEEALRTLSRFDASAEPLRAVAKHLLVRKG"
     gene            37308..39242
                     /gene="dxs"
                     /locus_tag="PLM79_00170"
     CDS             37308..39242
                     /gene="dxs"
                     /locus_tag="PLM79_00170"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOV84748.1"
                     /translation="MEEPQKSLLSRIDSPSQLKQLTLAQLQQLAQEIRETIIQTLSVN
                     GGHLAPSLGVVELTLALHFVFDSPHDRIIWDVGHQAYAHKILTGRRSQFHTIRQFGGI
                     SGFPKRNESRHDVFDTGHSSTSISGALGMTVANSLKGSRNRTIAVIGDGSMTGGMAFE
                     ALNHAGDLDKDLMVVLNDNEMSISPNVGALSSFLSRKLSNKTLLNLKREMESFLKSIP
                     GLGPNIVQLLRRSEDSLVTFFTPGMLFQALDFHYIGPIKGHRLDRLVEVLQAARQIKG
                     PVLVHVMTQKGKGYPPAEKDPTSFHGVGAFEVETGRSLNAKGGAPSYTHVFGETITSL
                     AERDTKVIAITAAMLQGTGLELFSKTFPDRFFDVGIAEQHAVTFAAGLAVDGFKPVVA
                     VYSTFLQRAYDQIIHDVCLQNLPVVFSMDRGGVVGEDGPTHHGVFDLSFLRHIPNMVL
                     MAPKDENELRHMLKTALDHPGPAALRYPRGNGMGVPLDENLQALPIGRGEVLREGPDI
                     LLIGIGSTVYPCLEAASKLEGQGIEATVVNARFVKPLDRELLLGLAEKIGRVVTVEEN
                     VLAGGFGSAVLEMFQEAGFFPGDFVRLGLPDSFIPHGSQSILRSMHGIDADGIQKAAL
                     GILQARHGQVLRAIGQVARR"
     gene            39220..39942
                     /locus_tag="PLM79_00175"
     CDS             39220..39942
                     /locus_tag="PLM79_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOV84749.1"
                     /translation="MDRLLVDKGLVSSRERAQALILAARVRVNGVPATKAGQSVREGS
                     QIELLGDLPFVSRGGYKLDHALDQFHVDVRGLTAMDVGASTGGFTDCLLQRGARRVYA
                     VDVGYGQLAWKLRQDERVVVLERQNIRHLSTDLVAEPIWICTIDASFISLRRVIPAAL
                     RFLVPGARLIALIKPQFEAGPSRVGKRGVVRDPAVHDEICTSLQRFVQDLGFEVLGIV
                     PSPILGPKGNREFLLGAVFPRA"
     gene            40435..40907
                     /locus_tag="PLM79_00180"
                     /pseudo
     CDS             40435..40907
                     /locus_tag="PLM79_00180"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
     gene            complement(41047..41265)
                     /locus_tag="PLM79_00185"
     CDS             complement(41047..41265)
                     /locus_tag="PLM79_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOV84750.1"
                     /translation="MREQVEQALEKIRPMLQRDGGNVELVGVDGTVVKVRLQGACHGC
                     PMSQMTLKAGIERIIKQEVPGVSEVVAV"
     gene            41808..42338
                     /gene="hpt"
                     /locus_tag="PLM79_00190"
     CDS             41808..42338
                     /gene="hpt"
                     /locus_tag="PLM79_00190"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOV84751.1"
                     /translation="MRQYRLVARISSQELERRVDELARQINRDYQGKQVLLIGVLKGA
                     YVFLADLARRLSFPVEVDFVRLSSYGNKAESSGSVRITKDIEVDIQGRHVLVVEDIVD
                     TGITLGWFLERLRERKPESLKLCALIDKLERREVEVPVDYVGIRLESGFLVGYGLDYS
                     ERHRNLPDIREVEFTD"
     gene            42413..43171
                     /locus_tag="PLM79_00195"
     CDS             42413..43171
                     /locus_tag="PLM79_00195"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOV84752.1"
                     /translation="MIVTCESCKTKFRLDPSLLKGPRAKVRCSRCGHTFRVAPPEEEV
                     LIHIDLSEDALGQEEAAPSRIGTPAPPAPVRPGKQGPFRKIWVLILPLVLIVAGAVYF
                     FSRSDSKPGSPSPPSSVEVKQPTVTILDSTQAYFLENSHAGQIFVVEGEVVNESPKPV
                     SFVLLEGKLYKRDNKVGQSQRCYAGNAMNRKELAQLSVTDIQNHMMNREGKDLSNVNI
                     QPSRRVPFMLVFHNLPEMDLLGDYSVEVVSAKLD"
     gene            43371..44651
                     /gene="serS"
                     /locus_tag="PLM79_00200"
     CDS             43371..44651
                     /gene="serS"
                     /locus_tag="PLM79_00200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOV84753.1"
                     /translation="MLDLKFVRDNIDRVNQMLEDRHMDTDLKAFLVLDENRRRILRRV
                     EELKYRRNVASEEISRLKREKKDASRQIGEMREVGEQIKALDSELAAIEEAFREVLLL
                     IPNMPHESVAVGLDEKDNPVVRTWGAAPEFSFDPKPHWEIGENLGILDFERASRMTGA
                     RFALYWDSGAALERALISFMLDLHTRRHGYTEVLPPFMVNSNSLLGTGQLPKFKEDLF
                     KIEGRDFYLVPTAEVPVTNIHANEVLDEETLPRYYTAYTPCFRSEAGSYGKDTRGLIR
                     QHQFNKVELVKFVSPATSYDELERLLANAETVLQELGIHYRVVMLCTGDLGFSASKTY
                     DIEAWLPGQNTYREISSCSNFEAFQARRANIRFRSKGRSKTDFVHTLNGSGLAVGRTL
                     VAVLENYQQEDGTVRIPEVLRPYMGNAERIGPRP"
     gene            44686..44994
                     /locus_tag="PLM79_00205"
     CDS             44686..44994
                     /locus_tag="PLM79_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV84754.1"
                     /translation="MTKSQLIEALAKAEGITLKAAETAVNVTFQSMELALIRSDRLEI
                     RGFGSFKVKYYEGYKGRNPKTGELIEVQFKKLPFFKVGKELRERVNGGEPEAESSKEK
                     "
     gene            complement(45089..45430)
                     /locus_tag="PLM79_00210"
     CDS             complement(45089..45430)
                     /locus_tag="PLM79_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84755.1"
                     /translation="MDQIKTAWQEFVRFLSEYDVTKIMDMIARVDWQKALSSPFTWLV
                     LVPVLGYLILRRHFRILLVSASVVVFLVFVQTRLPAAGEAIPVSKLMEFLGVAVLIIA
                     VNIYYLFMREK"
     gene            complement(45436..45723)
                     /locus_tag="PLM79_00215"
     CDS             complement(45436..45723)
                     /locus_tag="PLM79_00215"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOV84756.1"
                     /translation="MQKKRVRVTISGRVQGVFFRAYTRDAALAEGGIAGWVRNLADGR
                     VEAVFEGDEDKVARMIEWCREGSPFGRVDRVDVQEEVYRGDLESFDIRYGR"
     gene            complement(45737..47791)
                     /gene="ligA"
                     /locus_tag="PLM79_00220"
     CDS             complement(45737..47791)
                     /gene="ligA"
                     /locus_tag="PLM79_00220"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041440114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOV84757.1"
                     /translation="MNNEADRVRRELESLRREIRRHNYLYYALDRPEISDADYDALYR
                     ELLRIESAHPELVTPDSPSQRVGFPPLESFQPFEHAVPMLSLENAMEESEVFEFDRRV
                     KKLLGPAEDPAYVAEPKMDGLAVEIVYEGSTLVRAGTRGDGIVGEDVTLNVKTIRSIP
                     WQLFSSPEGQPVPTLLAVRGEVYMDRKDFEALNRSREAAGEPVFANPRNAAAGSLRQL
                     DSSVTAGRPLKAFFYGIGQWEGVDFQSQWEVLERLRQWGLPVNPRSRLCRNIQEAVDF
                     FRILEQERERLPYETDGMVVKVDRLDLQRRLGEKSRSPRWAIAYKFSPHQAESRILDI
                     RVQVGRTGVLTPVAVLEPVNVGGVVVQRATLHNQDEIDRKGILLHDTVRVRRAGDVIP
                     EVFEVVRSRRTGSEQAFRMPPWCPSCGQPVVRLPDESVHRCLNRNCPAQIKGAIWHYA
                     SRDAMNIDGLGKKIVSFLVEQGVLRSVADLYRLQPRDIESFPGFGKKSASNLVEAVER
                     SKETTLPRFLYALGIPHVGSHLAQLLAAHLPSIPSVMDAGEAQLTAIPGVGDVVAFSV
                     RTYFANPANRELVLDLLGSGIRFRESSPPAPVLEDFWTGKTVVLSGTLEKMTRQEAIG
                     MLTDRGARVTSSVSGATDILIIGKDPGSKLEKARTLGTQVMEEEEFLARLRGGARPDS
                     DG"
     gene            complement(47809..48651)
                     /locus_tag="PLM79_00225"
     CDS             complement(47809..48651)
                     /locus_tag="PLM79_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOV84758.1"
                     /translation="MPTHERPALIEPRKGRREDPLPPLALVVFTSQDLESLCRVHAGA
                     TRRDRTLFLADVRILGGSYGEVACVGPMIGAPQAAMVLEKMIALGVRRVVTLGWCGSL
                     QPGVRIGDVVLPTGAFSEEGTSLHYLEDPGKAAPSTRILGVLREKSRETGLVFHEGKV
                     WTTDAPYRETASKVRLYGEAGALAVEMETSALFSVAHFRGIELATVLVVSDELFGGKW
                     THGFREARFHRAREKLVEWVLEAVAGLWEAFPEGRDPDHRQTDGPVEGPARQAASGSE
                     PTTS"
     gene            complement(48741..49013)
                     /locus_tag="PLM79_00230"
     CDS             complement(48741..49013)
                     /locus_tag="PLM79_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV84759.1"
                     /translation="MTKTELVAKIAEGAGITKATAEKALNSFISAVGKALAAEDKITL
                     VGFGTFEVATRSQREGRNPRTGQTITIPSSKVVKFKPGSKLRESVK"
     gene            49691..52126
                     /gene="priA"
                     /locus_tag="PLM79_00235"
     CDS             49691..52126
                     /gene="priA"
                     /locus_tag="PLM79_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOV84760.1"
                     /translation="MFAEVAVFSAMEKTLHYLVPESMEHEMGAGKRVLVPLGRREAMG
                     LVLALDSSPPSLPKTVRLRPVLSVLDSQPVVPDELLSLCRWMSFYYFHPWGEVLETVL
                     PRELSVSPQVCFRLTPGAATAKASRQDREILLAIEEAGALCMGELAGRIPESTHLSRR
                     VRSLEGRGLLTRFFRWNSSRVSPKKVKTVRLISPPSPDFLSRSPNLGRLVGLLEESEG
                     PHPLPLLRHRVPNADYWVGRLAREGWVEVDTFQEVRESACAQDLARPSEIIPTREQQE
                     VVQSVLPSLLRRRFETFLLYGITGSGKTEIYLRLVEKAIEQGRGSIVLVPEIALSTQM
                     EPIFRHRFGADLAVWHSGLPSGVRYDQWREVLEGRKRIVLGVRSAVFMPVPDLGLILV
                     DEEHDPSYKQDDRLRYHARDVALMRAKALGIPIVLGSATPSLQSLHQCGLNRYRLLTL
                     SRRIMERPLPELVLVDMRRQKGRDRILSAELKEALRQTTADGKQALVFLNRRGFATFV
                     LCNTCGEVIRCSRCSVSLTFHQKQNLLRCHYCGMERSIPEQCPFCSRSSLFPLGFGTE
                     RVEEEIRHLCPRARVVRVDRDTVRRSGQMVECFNAVRNEEADILIGTQMVAKGHDFPG
                     ITLVGIVNADMGLQMSDFRAGETTVQLLMQVAGRAGRGDKPGRVLIQTYNPTHYTIRS
                     VLEMDYLGFARRELESRERLQYPPHARLTRFVLTSPREDAAGKAARELGLLCERIAGE
                     LRPRGVNIALLGPSPAPLTRLHNQYRYHLFAKSWTSREMQAFGEAVVRQMRLQPFLKN
                     VSCTIDRDVMNSL"
     gene            complement(52135..53289)
                     /locus_tag="PLM79_00240"
     CDS             complement(52135..53289)
                     /locus_tag="PLM79_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOV84761.1"
                     /translation="MNGPNGSGSEIPGAGKSYRPFLLVVLFFSLYLSYLILKPFLDTL
                     ILSIVLASLFYPLQVYLVRFYRGRKNLAALTIVFLFTFVIALPVFFFSTALVAQGLQS
                     VNQINEWLKAGNLLKLTHDPKILEYLDWFEEKFGSLELHKIDFQSNLLQVSKNVGQFI
                     LARGATLLGNAATLLMHFLVMIFVLFYLVRDGVEMIERGRYFSPLRKEQEDRILDTIR
                     MVARSVFMGCFLTALCQGIVGGIGLSLVGIPGLFWGTVLAFSSLIPLVGTALVWVPCA
                     LYLLLFGSVKSAVFLAAWFVLLVGSFDNFLRPYLMRGQGALSLSPFYIFLAIIGGVQY
                     FGLAGVLYGPLSLAFAMVMLYIYGVEYRGDLLAGDKAAPPMMNPGEPGRK"
     gene            complement(53302..53961)
                     /locus_tag="PLM79_00245"
     CDS             complement(53302..53961)
                     /locus_tag="PLM79_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84762.1"
                     /translation="MINESRPTGPPATKRHCGAVLWMLLCFLPAGCAPKVVQIPPEWA
                     QAPQPAPAPPKGETTTPFPGSSGLSGPPPATTAILKPPPAIKEKDLSTPSEPVPPPRE
                     KKQEPHPPQHLASMHLVDQAKGSLKQKKPDSAIPLLEQAIQVDVHNGEAFLNLARAWR
                     MKGFRSKALEFAKKAEILFQEEPAKLKEAYLLEAELYKEMGDSRKTDLYRQKAAKISS
                     P"
     gene            complement(53998..56235)
                     /locus_tag="PLM79_00250"
     CDS             complement(53998..56235)
                     /locus_tag="PLM79_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOV84763.1"
                     /translation="MTWLVAKVLILLFSLALLVAVAFFYHLTVEIQKRFSSRRWSVPS
                     RVFSAVVPVYPGQSLSLSDVRRLFEERRYQTAIRDTLNPGEFRVFPNSLVVHIRGFQF
                     PGRTLPAQTVRFDFQQNKISRIVSAGGDIPVLELEPMELARLFGPERESRLLVNIGQV
                     PRHLTEAIVAIEDHRFYEHGGLDWRGMFRALWADLRARRVVQGGSTITQQLVKNYFLE
                     PERSIKRKLLEASMSLVMENLYTKDEILEMYLNEIYMGQRGSVAVHGVGEASRTYFGR
                     NVEDLTLAESATLAGMIRAPNMYSPLSRPEASKKRRNLVLKRMLELEKISFLDHEKTR
                     LEPVRVADPSLPVNLAPYFVDTVRQQLQELYAPEALESEGLNIYTTLHPELALAAENT
                     LREGLKELEKAASTPPGSEPFPPLQAVLIAIQPKTGSVLSLRGGRDYWESSYNRALYA
                     HRQPGSAIKPFVYLAALDAFTPSDLLADEATSYPVDGTFWVPKNYDRRYRGRVLFRDA
                     LEQSLNAATVSLALTVGLNNIMETIRTLGIQTPLQPVPSLALGAFEVTPLQLAEAYAT
                     LDNDGQKPYLLTLREVVDDKGNVLERRSVDLATVTTPAKAFLITDLLQGVVERGTARN
                     VRRLGVNFPCAGKTGTTNDTRDSWFVGYTTDLLVLIWVGYDDNRPTELTGAKGAGRLW
                     ARFMSQVRPWIHPQKFRVPPGVVQRLICPQSGRLAVLSCPGRRFEYFLSENVPQDFCW
                     LHSRP"
     gene            complement(56309..57730)
                     /gene="rlmD"
                     /locus_tag="PLM79_00255"
     CDS             complement(56309..57730)
                     /gene="rlmD"
                     /locus_tag="PLM79_00255"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOV84764.1"
                     /translation="MTEIRKGSEIEVQVEKLAFGGSALGYLNGLVVFLDHGIPGQLVR
                     ARITRKKPQYVEASPVEVLRQSPHYRDPFCPHFSVCGGCRWQDIDYETQLLWKRSHVA
                     DCLERISGIEDTRTAPVIASPDQRFYRNKMEYTFSPRRWLSPLEIAAKDTVYDKSLAL
                     GLHIRGFYDKVFNVESCFLQSEASVELVRFVRKWCAESGIAAYSTRNHQGYWRFLVVR
                     EGKRTGESLAHLITAGIPEYAPRVDQLGREIASRFPWLTSFVHSVSSKKAQVAFGDSS
                     RVLFGPGAITERLGNLRYSISAHSFFQTNPLAAEGLYETVLRFAELTGKETVWDLYCG
                     TGSISLFVAPRVRRVVGYEVIEEAISDAYDNGRRNGIENCTFKAGDLKDTLGKDVESL
                     SPHEVPDVVITDPPRSGMHPKVLKTLLELAPERIVAVSCNPATLARDLCLLAEQYEIR
                     KVQPFDLFPHTPHIECVVQLERR"
     gene            complement(57846..59078)
                     /locus_tag="PLM79_00260"
     CDS             complement(57846..59078)
                     /locus_tag="PLM79_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOV84765.1"
                     /translation="MLPLHELKGEEIYRIRVACAFLFSVEQARDDAFVARLDLERVTR
                     AFREKAKRYHPDLHRDEPREMIERRKERLLKVMDSYDLLRSYLRTDPGREGEPASAEA
                     RKRLIAVGGAKGGIGKSLFAANLGVLLSSRGYRVVLVDLDLGGANLHLYLGKTFLPRC
                     INDFLEKRTPRIEDALTETQYGPGLIGGDSSRFGAANIGFSRKIKLIKSIKRIDADYV
                     ILDLGGDTSFNIIDFFLSADIGIVMATCDPAAYLEAYNFIKVALYRKLNRIFGEESDL
                     GLKKDERLARLIARVTSPADGRRAIPMSELVEAARKEQPQNLFLIQQVLQSYRPKLVL
                     NLVEDQSIMMDVAGRIQEVARKMLSTTVDFVGCLPLEAQVQHSARTLTPVTAQSPQGL
                     YASHLAAIAKEVGIRTGV"
     gene            complement(59163..59783)
                     /locus_tag="PLM79_00265"
     CDS             complement(59163..59783)
                     /locus_tag="PLM79_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84766.1"
                     /translation="MKDVFSSDKEISATERLLQAIRGESSSKDASPPSPLSETSPPSP
                     WSRLSARGGLLPFPTRTTLGIDLGHQQLRMVLIAQTGDHRWNLLGYRRVPLPTTAPGD
                     SSELAEFLNAAVQDFAGSSKKMRVWGLISAAHVDVRHVRIPKVPRKQIYNAAVGRPGE
                     MPASARRPSREPEEPDGFYELKEDLSVNEIIDKIQNWRKVPPKVEP"
     gene            60397..61272
                     /locus_tag="PLM79_00270"
     CDS             60397..61272
                     /locus_tag="PLM79_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HOV84767.1"
                     /translation="MSPWIQKLRTYGRLVKFSHTVFALPFALSAVLLAHREHPLTGSV
                     VLWVLLAMAGARSAAMGFNRLVDYEYDRLNPRTSDRPLTSGQIDRSSVKMFIAGSSLL
                     FILSATALGKLCFLLAFPTLAILFAYSYTKRFTPYSHFFLGLAIGLAPLGAWVAVSGA
                     LDPRILLLSLALLTYIAGFDLLYACQDIDFDRTNRLFSYPASYGVRRTLRLSSALHCI
                     TFVLLTALYFAFDLGIPYLGFLVLIGFLLALEHRLVRPDRLDRINLAFFHVNSLVSIL
                     LFLAVWTDLWLGARG"
     gene            61371..63215
                     /locus_tag="PLM79_00275"
     CDS             61371..63215
                     /locus_tag="PLM79_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOV84768.1"
                     /translation="MTTAYRNLREFVEALDRAGELLRIPAPVSRHLEITHITDLASKA
                     PAGGKALLFERVEGFDFPVLTNAFGSERRICMALGVSDLESLAGRLRRFIEIKPPKSL
                     KDGLKLLPLGLDVFRFLPRKVRRAPCQEVVYRGRDADLGKIPVLQCWPQDGGPFVTLP
                     VVITRSLETGRRNAGMYRLQVYDARTTGMHWHIHKDGSHYYQEYRRAGKRMPVAVAVG
                     TDPATTYAATAPMPRGIDEMLLAGFIRRKPVPMVQCITVDLQVPAEAEFVLEGTVDPK
                     ELRIEGPFGDHTGYYSLVGDYPVFHVTAITHRRNPIYAATIVGRPPMEDCYLAKATER
                     IFLPLLNTVLPEIRDFWMPWEGVFHNITVLSVEKEYPGHARKVMNAVWGQGQMSFSKV
                     LVLVDSDVDLHRPREVLETVLDTVDLASDLYVTEGVLDVLDHSAPEPLFGGKLGIDAT
                     RRIEGERPRVRPTGVVSPVEPSRIEKALREVSPNLRSFCVPELRARNPLLLLNLEKDG
                     KTTGKAVGQRILEHKGLESFALFVLLDAAVDLRDSSLTLWKLFNNVDPLRDLVRRDRR
                     VVLDATRKGPEDGHHRPWPDDIVMDPKVAARVRERARELNIEPFLKKE"
     gene            63407..63910
                     /locus_tag="PLM79_00280"
     CDS             63407..63910
                     /locus_tag="PLM79_00280"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV84769.1"
                     /translation="MPEHIRRHSHRVAAVALYLGRLLNENGCRLDLRLVEAGALLHDV
                     GKSQALATGERHEMLGAEIVGRWGYRPLVPIVRDHVHLDPATVRGPITESVLVNYADK
                     RVKHDEIVSLAERFRDLVERYGRTPKHIEWLEEKYRLFDRLEKKIFGHLGIHPQEVGL
                     MSLGTNP"
     gene            63933..64871
                     /locus_tag="PLM79_00285"
     CDS             63933..64871
                     /locus_tag="PLM79_00285"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOV84770.1"
                     /translation="MGKKLTVALLAGGKSAEREVSLKSGEQVFRALDKSRYEVLRYDP
                     RDDLPRLAADAGKIDVALIILHGRLGEDGTVQGFLDLLGIPYQGSGVLGSAIAMNKIL
                     SKQLYIQAGLKVAPYGILDSRQREAGMETVRNLGFPVVVKPEHEGSSIGLSIVNREED
                     LAGALEAAWEFDRRCLVEKYVRGIEITAGVLGNDHLQALPLIEIVPGEGHAFFDYQAK
                     YTPGASSEICPARISPELTALAQDHACRAHRALHCKGYSRTDMILSPEENEVYVLETN
                     TIPGMTQTSLLPQAAAAAGISFSSLLDRLITLAMEP"
     gene            complement(64868..65497)
                     /locus_tag="PLM79_00290"
     CDS             complement(64868..65497)
                     /locus_tag="PLM79_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84771.1"
                     /translation="MNFRIERVEHRPEEDLVRNLRQVTMLRRRDVKVYEKADIRLETL
                     GVDRLHPAQRYILVQGLMKVRQLKWELARLQYDLFRLDGYLKVWIEGGTEPVDLLPPV
                     VEQWVEANGKTVNIINDGMHRLYLAYLEWIDPQVILIRNVPREYPYYAFPNPTQWQGI
                     DWMDSLPEGYVKKWHRIADYKTLYRDFNSAFDNVGAPRGRFDKAGSAAT"
     gene            complement(65580..66236)
                     /locus_tag="PLM79_00295"
     CDS             complement(65580..66236)
                     /locus_tag="PLM79_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV84772.1"
                     /translation="MAKAKILITDDHRVVIEGIKSALRDHEDFEVVGEAVNGRDAVRK
                     VRTLEPDIVIMDISMPDLNGIDSTLQIKKINPDVAVIIFSMYSSKEYVIDLFRAGISA
                     YILKEDPMSQLLAALKEAKAGRKYFSEMVQQIMLDHLTNAGPGQAMAKDPFEGLSLRE
                     REVFQLLAEGASIKDIARRLNLSPKTVESHKYHILQKLKVQSVNDLTKMAIRKKIISI
                     "
     gene            66533..66715
                     /locus_tag="PLM79_00300"
     CDS             66533..66715
                     /locus_tag="PLM79_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84773.1"
                     /translation="MAEKREVCTCTKCGNEAEMIVTCELVEIETSPGTKQKKQKENRK
                     CTYCGNEADMIIDFDE"
     gene            66997..67515
                     /locus_tag="PLM79_00305"
     CDS             66997..67515
                     /locus_tag="PLM79_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84774.1"
                     /translation="MARAGGSHACIRVTEESGARRLMCGDFVVGLTSAGFVFSAFDEH
                     RDLKTYEGSAACTPCHVEEAREVHASAHHQWKGKTPQLPDLGFAGKLGGINDFCTYPN
                     LNFLFLMTNLGGATVGAGCAACHPGMGAKPQRGATAEQPANVDCLLCHSDAYKRVAVK
                     QTAGMRFVPDPP"
     gene            67518..68180
                     /locus_tag="PLM79_00310"
     CDS             67518..68180
                     /locus_tag="PLM79_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84775.1"
                     /translation="MGSVPANIHKPTNSTCLNRCHVRAGGSPGGKQRDIDLTMENPPR
                     TLDVHLSASGANLSCLSCHKAKNHRIAGRGNDVRETDLAKKIACADCHGAAPHRDYDL
                     DAHTGRVDCTVYHIPRYARGPNPTETHRDARRAKSDPATLRYEPPRVFASNLVPEYLF
                     RNGDSHFYKFGDPVQLQPNGRLLLTGPLGDIREPSAKRSLLSNPTRPGWGTIRSPGAS
                     CR"
     gene            68141..68557
                     /locus_tag="PLM79_00315"
     CDS             68141..68557
                     /locus_tag="PLM79_00315"
                     /inference="COORDINATES: protein motif:HMM:NF023211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOV84776.1"
                     /translation="MGYHPVTWRLLPVKSKVLWETGKPIEAFVAGAKDLGWKLPQGVG
                     FVSSDRYLSLHHQVAPKRYALECAEFRGGTRMNFERLGYAPKTTRDGTPLCISCHDRE
                     DDKPDYYEIHAIHPREEGVNCKTCHKFSGTREGWED"
     gene            complement(68672..68896)
                     /locus_tag="PLM79_00320"
     CDS             complement(68672..68896)
                     /locus_tag="PLM79_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84777.1"
                     /translation="MELNIEALRKERRPLWMTHLDVESYADFLGGGGDVHRDRHLPEW
                     ERFAGVEEEEDLEALRILLEGRYVAEDYIS"
     gene            complement(69029..69793)
                     /locus_tag="PLM79_00325"
     CDS             complement(69029..69793)
                     /locus_tag="PLM79_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV84778.1"
                     /translation="MKKSWNWRRQPPGNCAAANKGVALRRRTVGRVIETPRLILRGME
                     PTDRDYLCGMFASRETMKYLFGGVPMGREEAVEFIGRHFPFGAEPAGPAPLCLKETGE
                     RIGFGGIVPCGHLSEDDYEFGSALVKSARGRGFAVEIGGARIHYGFRHLGCSRMLALV
                     HPMNEPSKAVLKRLGMRFYGEVPLPDRGPREIHILKEANGSQPHPPSRPRPSNKHRPK
                     VSDLAVMSPPFSFPRRREFAKRVHPRRMPAVADMTP"
     gene            complement(69735..70313)
                     /locus_tag="PLM79_00330"
     CDS             complement(69735..70313)
                     /locus_tag="PLM79_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV84779.1"
                     /translation="MKIVCLLGSPRRKGNSSMIAERFCSTAQGLGAEVRTFALNDLSF
                     RGCQGCMTCKTRLDRCALEDDLTEVLDAVRATDLLVLASPVYYWDVSSQMKAFLDRTY
                     SFLVPDFIVNPRKSRLEPGKKLVFILAQNNPDRNSFTDIFKKFDYFFRAYGFSDTRLI
                     RAFGVAAPGEVERCEEVMELAETTARELCGGQ"
     gene            complement(70428..70503)
                     /locus_tag="PLM79_00335"
     tRNA            complement(70428..70503)
                     /locus_tag="PLM79_00335"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70468..70470),aa:Phe,seq:gaa)
     gene            complement(70611..>71341)
                     /locus_tag="PLM79_00340"
     CDS             complement(70611..>71341)
                     /locus_tag="PLM79_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84780.1"
                     /translation="LELDADVLEEVPLPATQAPAASPAAAPESKETAGTPGLSLDLED
                     APTLQISPEEMDRIVKSARHPSEKVPEPDFSLDLDFDAQEKTPAGPMVGTIPGPVPEL
                     DLKLEEEPTLRLEPLEYPSPRPVSAPPAPEPALEVPEITLDLDEAFVQGKPPTSEFEL
                     VIDEEELRQTMELETTADGIIGLEPIVPDRPREAPTELPTEPLEISDSDLVLELTDDD
                     LDGLLEELEETSPKGEKKPPVKPS"
BASE COUNT        14076 a        21781 c        21859 g        13625 t
ORIGIN      
        1 cctggacggc agaacggtgc ccaaggtttc catagcggaa ctcgctcgag cctgcggggt
       61 cgggttcgtg gaagaggaag acccctaccg gttcaagccc ctggtggatc tgctcaaacg
      121 ggcgggaaaa cacgttcgga gttcccgggg gtccgtggcc gtggtgatcg ctcggcggcc
      181 ctgcctcatg gaccggggcc gaagcgacgg ctgggagcgc ttcgacatcc gggtgaacga
      241 gaaatgcaag gggtgcctct tctgcgtcaa gcagtttgaa tgcccggctt tgcagccccg
      301 ggggcaaaag gagcccattg tgctggaccg gacgttgtgt cggggctgtg gggtctgtgt
      361 tcacgtctgt ccccatggag ccctcgagat cgcctgacgg ccgagggacc ggtttttccc
      421 tatccctgcg ggagtaccat accaagccta tgagtccaat accgagacag cagatcattg
      481 tgagcggggt cggggggcag ggagtgcttt ttgccacgag gatcctcgcg gaagccgccc
      541 tggccgcgaa ctaccacgta cttctttccg aaacccacgg gatggctcaa agagggggca
      601 acgtcatcag ccatgtgaag atcaccgggg tggaagtgcc ccccttcacc agccccctca
      661 tccgaccggg tcacgcggac cttttcttgg gtctgcatcc gcaagccttt gcggttcacg
      721 gtttttacct caagcccgac ggaagagcct ttcgcaacga ggcacgttcg gaggaccccg
      781 attgcctgga tgccgctgaa attgccgcct ccctcgggtc ctccatttcc gtaaacctgg
      841 tcatgctggg atttgccgcg agctcgcggc ttctcttctg cgatgcgatc caactggagg
      901 agaccgtgcg aagcctttcg ggcccgcgcg ccgatacggc tctccgtctt ttccgtgccg
      961 ggctcgatcg gggtcgcgcc ctttcactct agctgccgta tcgccggacg aggcatgtca
     1021 tgtggtggaa cctctttcct cagttcttcg tttccggcat caccagcggc agtctgtacg
     1081 ccctcatcgc cctgggctat tgtctcatcc agaacgccac cggcctggtg aattttgccc
     1141 agggggaatt cgtcatgctg ggagccatga cggccatcgc cttgcgccag gcggtcccgt
     1201 ccctccccct tcccgtggtt ttcgccctgt ccatggtcct ggtcgccctg gtcggaatcc
     1261 tcctcgagag gggtcctttg cgccgctccc gaaaccggga cattctcacc ctggtcatga
     1321 tcaccgtggg cgcctccatc tcccttcgcg gagccaccat gttgctgtgg ggtaaaaacg
     1381 cccatgcctt ccctcccctg ggcggaagca ctcccatgct tttctggaaa gcggccgtcg
     1441 ttccccagac cctctggatt ctcttcctgt ccgtggtcgt cctggttctc ctgtacggtt
     1501 tttatcacca caccctcttg gggaaagcca tgaggggagc ggcggacaac cccgtgggcg
     1561 ccacgttgat cgggatttcc gtgggccggt tggtggccct ggccttcggg atgagcggag
     1621 ccctgggagc cctggccggg attctcatca ctcccctcac caccatgagc tacgacacgg
     1681 gcctgatgct ggggctcaaa gggttcgccg gggcgattct cggcggctac gggagtgttc
     1741 cgggagcggt ggcgggagga ctcctcctgg gaattctcga atccttcggc gccgggttca
     1801 tctcttcggc ctacaaggac gcgttggcct ttctcattct tctggggatt cttttcctgc
     1861 gaccggcggg actttttggc agcaagacgg tgaggcgcct atgaaaccgg gaagaacgct
     1921 ctccgccgcc ccgccgcccg gcctttccaa cgaagacggg agtcgacgag gatcggtcgg
     1981 gacgcctttc cttccctcgg attggcggat gctgggcgcc ctcaccgccc tggtcctggc
     2041 tctccccctg atcatccgaa cggactacac gatccttttg ctgaatatca ttgggttgaa
     2101 ctccctggtg gttctgggac tgaacctgat gattcgtgcg acggggcagg tctccctggg
     2161 gcacgcggcc ttttacggtt tgggagccta tgtttccgcc gttgtcggcg tcacgttcca
     2221 gtggccgatt cttccgtccc tgctgctggc cttggcggtg gtgaccctca tgggatttct
     2281 cctgtccctc cccaccctgc gcctcgaggg tcattacctg gtcatggcca ccctgggatt
     2341 caatatcatt gtgaccatcg tcttcaatca gatggaggac atcaccggag gcccttcggg
     2401 gtttcccgga attcccaagc tgagcctggg gccctggaga atcgcttccg accgggactt
     2461 ctactttttt ctctggcccc tggtgatcct tctcctggcc ctgagcctga acctgatgaa
     2521 ctcccgcacg ggtcgggccc tcgaggcgat ccacgaaagg gagctgacga cccggaccct
     2581 gggaatcccc accaagagat acaaggtggc cgtcttcact ctgagtaccc tgtacgcggg
     2641 gatcggcggt ttctgctacg cccattacgt caccttcatc agtcccaaga cctttgacat
     2701 cttttactcg gtccaactgg tgaccatggc ggtggtgggg ggcatgggaa acctgtgggg
     2761 aggactggcg ggggcggcgt tgctcacttg ccttcccgag ctgctccaga agttcgagga
     2821 tttctacgtg ctgctctacg gcctgatgct tatgggagtc ctagtgttct ttccccaggg
     2881 cctgttgcct tttctcactt ctcttcccgg gaggtggaaa aagagcccga gggacccttg
     2941 ggaagttccg tcttcttctc ggcccgccga ggtgggtgct cccggggaaa ggaacagccc
     3001 cgaggggccg gatgaggacc ccggctccgc cgcacccgtt catcaaggtc ctcccgccat
     3061 gagcgtccgg gacctttccg ttcacttcgg gggcctccag gccttgtacc gggtgagcct
     3121 cgaggtccac cccggccgga tcgtgggact catcggtccc aacggggccg gaaagaccac
     3181 cctgttgaac gtcgtctcgg ggcttttgaa gccggattcg ggagaagtgt tcctgaagga
     3241 gcggtccctt cgcgggttgg gaccccacga gattgccgca gccggtctcg gccggacctt
     3301 ccagagcgtg caggtctacc accggatgaa cgtcctggac aatgtgctcc tgggacttca
     3361 tctgcgcgga cgctcagggt tctgggcggg ctgctggcgc actcccggag ccgggagaga
     3421 ggaaagaagg ctcaaagagg aagccctcgg gcttctcgaa tccttcgggc tgggatcccg
     3481 ggaacactgg cccgtgggaa ggctcagcct cctggaacaa aagctcttgg aactggcccg
     3541 ggcccgggcc tccgacccgt cggtcctgct gctggacgag cccgcaggag ggctgaaccc
     3601 ccgggagagt caatgcctgg tggaacggat ctccgagctg cggtctcaag ggctggccat
     3661 ggttctcgtg gaacacgaca tgaacgtggt catgggcctt gccgaccggg tggtggtgtt
     3721 gcaacacggg acccgcatcg ccgagggcac gccccgggaa gtgcagcagg atccgcgggt
     3781 cattgcggcg tacctgggaa accgaaagcc gggagtgcgt tgagatgcgg aacattggag
     3841 tcgaacgccc atgcttcgag tagccggcct atcggcccgt tacgggtcca ttcccgtcct
     3901 tcgaaaggtt tcctttcacg tagaggcggg agagatcgtg gccctggtgg gatccaacgg
     3961 ggccgggaag agcactcttc ttcgaaccct cagcggcctt catcccctca ccgacgggac
     4021 catcctcttt cgaggccgcc cgattcagtc cctgagcccc gccacgatcg tgcggctggg
     4081 actggtgcag gtccccgagg cccgccagct cttccccaac ctcacggtcc tggaaaacct
     4141 cgagctgggg ggctacattc acgggaggaa gcatgtggcc cgttgcctcg agggaatgtt
     4201 ttcgctgttt cccgttcttt cccggcgcaa aaggcaaaag gcggggacct tgagcggcgg
     4261 ggagcagcaa atgctctcca ttgcacgggc cttgatggca aaacccgcgc tgttgctcct
     4321 ggatgagccg tccctgggcc tggcccccct ggtgatcgac gagatctacg gggccgttct
     4381 ggctttgagc gcccaggggg ttacgattct gctggtggaa cagaacgcca tgggcgccct
     4441 gggtattgcc cggcgggcct atgtgctgga aaacggaaga atcgttttgg caggcgattc
     4501 cgaggagctc atgggccacg acgacgttcg ccgggcctac ttgggcagag actatcggca
     4561 caaatgggaa aggtagagcc gtcatgatgt ttcccgaccc tttcggtccc atatcccggg
     4621 aagagatgcg ccaatggcag ctcgagcgac tgcagacgac cctgaatcga gcctacttcc
     4681 aggtggattt ttaccggaag cgcatggacg ctttgggctt gttgcccgag gacgtggccg
     4741 ccctcgagga tttgcgcacc cttcctttca cggcccgccg ggacctggcc gaccattacc
     4801 cgtacggact ttttgccgtc ccgttgcgca gcatcgtgcg tctacacacc agcacttcga
     4861 cctttcaggg gaaagaaaac cccatcgttt tggggtttac ccggcacgac gtggaaatgt
     4921 ggcgctccct ggcggtccgc cttttcgagc aggtcgaggt gacggatcga gacattgtcc
     4981 aggtggcctt caactacagc ctctttcccg gcgccttcac gttcaaccag gccgtggaag
     5041 ccctgggggc caccctggct cccgcggcca ccaattccgc caccattcag ctccaggtcc
     5101 tccaggattt ccgttgtacg gtcctggcca ccaccgcggc attcgccctc catattgcgg
     5161 acaccatgga acgaaccggc accgagtcga gccgacttcg ccttcgcttg ctcctggtgg
     5221 gacccgaacc gttgcccgat cccgctcgcg cccggatcga gtccgtgctg ggagtcccgg
     5281 tctacgggct ctacggcgtt acggaaatgg tggagccggg attggccggg gagtgtaccg
     5341 cccgggacgg gctgcacctt gccgaagacc agttcttggc cgaagtggtg cacccggtca
     5401 cgggggaacc cctgcccgcg ggaagcgagg gagagctggt cctcacgacc ctcacggctg
     5461 aaggatatcc tctcattcgt taccgcacgg gggacgtcac ggtccttcgc gaagtcccct
     5521 gcccctgcgg gaggaaaacc gcccggctct ccccggtgct tcgccgcacc gacaaccggg
     5581 tttccgtccg agggatcccc ttttacccgg aagacatcga gcttctcctg cggcgtttcg
     5641 attcgggtct cgaggacttc cggctggtca tctcgaccgt ttacggcatc ggggaccaga
     5701 tcgaggtcct gctgacccgg actcctgccc aagatcttcc cgggggcggc aggtcccagt
     5761 atttggacct cttaagaagt caattgcgac gagccttggg actgggagtg cgggtgcaga
     5821 tggtggagcc cgaccaattg ccccgggaag ggttgggata ccgaacggtt tttagaagct
     5881 tgacccccgt tcccgattcc cccctaccgt cgccgccctg aaagggagcc aagatccatg
     5941 gccgtcgccc atcccccgga gtccattgcg gactcgggga gatgggcctg caatcacttt
     6001 ttgtcaggca ggcaaggttt gtgttatgca gtataggttc ttcggcaacc cgcggcggga
     6061 aggccggact cgagcagggg tccgcgtgtt ttccgacggg catcgggggg cgattttatt
     6121 tccatgagaa aaggagatca tacatgaggg tttccttcat gccccagggg tcggtccggg
     6181 aagacctgcc ccgcattcag cttcaaggcc ttcaatggac ggtttcccac tgctacgaaa
     6241 attccccctt ctaccgctcc ctgttggatg cggcgggagt caagccggac cgggtccgca
     6301 gcctggaaga cctgaaacgg ctcccgttca ccacggccca ggacctcaag gaaggatacc
     6361 cgtttccgtt gtgcgccgtt ccccatgaga aaatcgtgcg cattcatgcg tcctcgggga
     6421 ccacgggaaa gcgaaaagtc ctcgcctata cccaaaagga cgtgaacgac tgggccgaca
     6481 tgttcgcccg gtgttacgag atggccgggc tcacccgtac ggaccgggtg caaattgcgg
     6541 tgggctacgg cctctggacg gcgggggtgg gctttcagct cggatgcgaa cggttcggag
     6601 ccatggccat ccccgtgggg cccgccaacg tggaaatcca ctgccagatg ctccaagaca
     6661 tggagagcac cgtgttttgc tccaccgctt ccatggccct cctcatggcc gaagaaatcg
     6721 agcggcgggg actacgcaaa gacattcgtc tcgagaaagt catcatgggc gccgagcggc
     6781 acagcaggcg catgagggaa cggatccggg agatcaccgg ggtcgagcac atattcgaca
     6841 ttccgggcct gacggagctc tacggcccgg gcaccggtct cgagtgcgtc catcacgagg
     6901 gaagccatta ctgggcggac tattacatcc tggaaatcct cgatccggat accctggaac
     6961 cggtgccccc cggcgagatc ggagagatgg tggtcaccac cctgcgcaag gaagccgcac
     7021 ccctcattcg ctaccggacc cgggacctga cccggctgct tccggacccc tgcccttgcg
     7081 gcaacccgtt cccgcggcac gatcatctcc tgggcaggtc cgacgacatg ttcatcttcc
     7141 gagcggtgaa catttatccc ggacagatcg accacgtcct ttcccaggtc cccggaatgg
     7201 gcagcgagta ccaggttcac ctgtcccgca gggaagacgg cagggacgtg atgctcatca
     7261 aggtcgagcg gctctgggaa gacgctcccg accgggaccg ggccttggcg gaaatgatcg
     7321 cctccgagat ccgcaggcag atcctggtgc gggcccaggt cgagatcctc gaatacggag
     7381 tcctgccccg caccgagcgc aagagcaagc gggtgtttga ccaccgcccc atgcaggatt
     7441 gacggtcgga cggcccggac cgggtcccgt gcgacaagcg gtgtcgaacc ggggtccgta
     7501 atgaggaccc catgggggtc ctcggagacc cccggaggaa gagtcgaaca acaaggagaa
     7561 accatgaaat cgaggactcg tttcattgtt ccgaaagttg tgcgggccct ggcgcttttt
     7621 gcgggcctga ttctcgccgt tcagaccgtt tccgcccagg aacccatccg gatcggggct
     7681 ttctttgccc tctcgggtcc ggcggcctcc atcgggaccc ccaccaagct cgtggcccag
     7741 atggtggtcg aaaagatcaa taaggaaggc ggcatcgatc agaggcccat tcagctggtg
     7801 ttcggggata cggaaagcga tcccaccaaa gcgctcatgg aagccaagcg cctggtggag
     7861 aaggagaagg tcgtcgcgat catcggcccc acccgcacgg acacgggcat ggccgtgaag
     7921 ccgtatatcg aggacacggc aaaggtcccc atcctcatga ccgtgggcgg ggatccggtc
     7981 atcgcggggg gaaaatttgg acccttccac tggaccttca agacccccca gcggacttcc
     8041 gtggcggtgt ccaaggttta cggctacctc aagtccaaaa acctcagcaa gatcgccctc
     8101 ctgaccgctt ccgacgggtt cggcaaagac ggtctcctgt ggctgaacca gctggttgcc
     8161 gactacggtc tgaccattac cgccagggaa tccttcaacg tgaccgacac ggacatgact
     8221 cctcagctgg taaagatccg ggagagcgga gcccaggccc tcatctgctg gaccatcggc
     8281 ccggccgggg cgctggtggc caagaacgtc aagcaactcg cccttcccat tcccctggtc
     8341 caatgccacg gccagccgga ccccaaatac atcgaactgg cgggagatgc ggccgagggc
     8401 agcatcatgc cgtccaccaa gctcatggtc gcggaccagc ttccggaaag tgatccccaa
     8461 aaggccgtga ttcgagagtt tctcagcctg tacgagggaa cctaccagta tcagaaacag
     8521 taccccgtca acacccattc cgggtacgcc tgggacgccg tctatcttct cgccaacgcc
     8581 atccgccagg cgggaaccga ccccgcaaag atgcgggacg ccatcgagca gacccggggc
     8641 tatgtcggaa tcagcggcat ctacaatctg accgcccagg accacaacgg actggggacc
     8701 gactccctgg tgatggtccg ggtcgagaaa ggaaagtggg tgctgctgca agactaggga
     8761 cccccgggat accccgtgga gtttgggaag gtcgtttgat ggaaatctac cgtcgtctgc
     8821 tgaacctggt gacgccccat tggcggcgcc tggccctggc catgatctgc atgttggggg
     8881 tggccgcctg cacggccgca tcggcctatc tcatcaagcc cgtgttggac gatatcttcg
     8941 tcttcaagaa gatgggcatg ttgaagatcc tgcccctgct ggtcctgatg ctgtttgtgg
     9001 tcaagggggt ctgcgcctgg gggaacgcct actggatgag ctatgtggga cagcacatca
     9061 tcgcccagtt gcgccaggac ctctacagtc acatgcagac cctctccctg tcctttttcg
     9121 accgaatgcc cacgggggtg ctcatgtccc ggatcaccaa cgacgtgagc cagctccagg
     9181 atgccgtcac cgattccatc accggcctgc tcaaggacag cttcagcatc ctgggattga
     9241 ccgtggtggt gttctaccgg gactggcaac tggccctcat cgccatggcg gtgctgcctt
     9301 tcgctttcta tcccatcgtg aagtttggaa gaatgctccg aaggatcagc cgcagcagcc
     9361 agcagtccat gggagagatt tcggtcatcc tccatgaaac catcagcggc aatcgcatcg
     9421 tcaaggcttt cggcatggag gaatacgaga tccggcggtt cggggaggcc aaccggcgct
     9481 ttctcggcta tgccatgagg tcggccgtgg tcaaggccat gtcgtccccc atcatggaat
     9541 tcctgggagg gctgggcatc gtgttcatca tctggtacgg gggctacagc gtcatccaag
     9601 gggtctcgac cccgggaaat ttcttctctt tcctggcggc cctcctgttg ctctacgaac
     9661 ccgtcaagcg gctcagcaag atgaacaacg ccctccagca gggggtggcc gccgcagtgc
     9721 gggtgtacga gatcctggat acggaaccgg aaatccggga ccggcccaat gcgtccgtcc
     9781 tgggtcccgt gtcccggggg atcagtcttc gcaacgtgtc cttccactac ggggatcagc
     9841 ccgttctcaa ggggatccac ctcgagatcg gcgtggggga agtggtggcc attgtgggag
     9901 tgagcggggc cgggaaaacc accctggtga atctcattcc ccgcttctac gacgtgaccg
     9961 agggagccgt tctcatcgac gggacggaca tccgggaggt tacgctggcg tccctgcggt
    10021 cccggatcgc catggtgacc cagcagagca ttcttttcaa cgacacggtt cgaaacaaca
    10081 tcgcctacgg agacgttcgc aagaccgacg aggaaatcat cgccgcggcc aaggcggcca
    10141 atgcgtacga cttcatcctg aggctgcccg aagggttcga caccgtgatc ggggaacaag
    10201 gcatgcgcct ttcggggggc gaacggcaac ggatctgcat cgcccgggca ctcctcaagg
    10261 acgctcccat tctcatcctg gacgaagcga cctcgtccct ggacagcgaa tcggaactcg
    10321 aagtccagaa ggccctcgaa aacctcatgg ccgggcgcac gactctggtc attgcccatc
    10381 gactctcgac ggtcaagaac gccgaccgga tcatcgccct ggtggacggg cgcatcgccg
    10441 aggaaggccg ccacgaagag ctgctccggg gagacaccga gtaccggcgg ctctacgagc
    10501 tccagttttc ccagtatggg gccgactcgg cgggataagg tccttcgggg gtcgagcccc
    10561 tgcgggaatg aggcaaaggg gcggtgcagg caaatatggg cttgacttcc tccggaggct
    10621 tcgagtacat atcagctggt taaggtcgtg aagcgggtgt agctcagctg gtagagcaca
    10681 agcttcccaa gcttggggtc gcgggttcga atcccgtcgc ccgctccaac cttagcgata
    10741 ttccttctcg gtgagaaggc ttcccttatt gaggtggctg caggcccggg aaacgaggcg
    10801 gcggcatgat cacttcgatt agtggatgac aagacttcta gattcccgac atagagcaaa
    10861 ccgaacggtt tcaatttaga tttttgtcgc tccgctaaaa gaagtgggta tgtcacccac
    10921 tttttttgtt tttgtactgg ggaaacgagg ctccgatgca gggatcgggg gaaaagggag
    10981 aagggctccc ggagcaaatc cggcagttgg tcgagcccgt ggtcgagtcc gaggggaaag
    11041 agttggtgga actggaatac cggcgcgaat cccacggctg ggtattgcgt ttgttcctcg
    11101 accaggaaga cggcatcacc gtggaagact gcgcccgggt gagccgggtg gtcggggacc
    11161 ttctggatgt cgccgacctg atcccgaatc cttaccacct ggaggtttcg tccccggggt
    11221 tgaatcggcc cttgcgcaag gtggagcatt tccggagcgc cctgggcaaa atcatcgaga
    11281 ttcggaccct tgcacccctg gaaggcagga agaaattcaa gggcacgttg ttggaggcgg
    11341 ccccggagtc cgtccgattg gactgcgacg gccgagctta cgaaatccct ttgggtctca
    11401 tggatcgggc tcgactctgt tacttcgagt ccaccgaaga atgacggaag ctgctcgctg
    11461 cggatcgatg cacatggggc taatggtaga aatggtgagg agatcgggac tatgacaggg
    11521 gagctgaagc ggttgatcga tcaggtcagt cgggagaagg gaatcgaccg ccaaaccttg
    11581 atccacactc tggaagaggc cattaagtcg gccatcaaga agaagcacgg cagcaagctc
    11641 gacctggatg tgaccttcaa cgaagagttc ggcgagatcg aagccttcct gttcaaggaa
    11701 gtggtggaac aggtatccga cccggaaaag caggtctccc tggaggaggc cctgcgcctg
    11761 gaccccgaaa gcgagatcgg agatgagctg gggatccgca tggatacgga agccttgggc
    11821 cgcattgccg ctcagtcggc caaacaggtc atcatccaga agatgaagga cgccgagcgg
    11881 gaagtcgtct tcgaggaata caagggccgt aagggtgaga tcgtcaacgg aatcgtccaa
    11941 agggtggaca aggggggcat cgtggtcaat ctgggccagg cggaagccct gctgccgccc
    12001 aaggagcaga tccccaagga gatctaccgc cagggggacc gaattcgggc ctatgttctc
    12061 gaggtgcgca aaatcagcaa gggaccccag atcatcctca gccgaactca tccccatttt
    12121 gtgatccaac tgtttcacgc cgaagtcccc gaaattgccg aagggatcgt cagcatcatc
    12181 agtgcggccc gggaaccggg atcccgggcc aagatcgccg tggtgtcccg ggacccggac
    12241 gtggaccccg tgggcgcttg tgtgggcatg aagggcaccc gggtgcaaaa cgttgtccag
    12301 gaactgcggg gagagaagat cgacattgtg ccgtggaaca tggaccccgc caagttcgtg
    12361 gtgaacggtc tggccccggc gatcatttcc aaggtcatca tcgatcaggc caacaggaac
    12421 atggaggtca tcgtgcccga cgatcagctc tccctggcca tcggccgcag ggggcagaac
    12481 gtacggctgg cttcccgcct gaccaactgg catatcgacg tgaagagcga gagccgctac
    12541 gagcgacaga aacaggcagg gtatcagtcc ctcctcgaga tccccgggtt gaccgaagag
    12601 attgccgacc gcttgtacga agccgggatt acctccctgg atgacttcgt ggaagcgagc
    12661 ctcaccgaac tcgaggactt gagccgcctt tcccaatcgg acctcgaggg catgcaggcg
    12721 aaagcgcgag aaaagctgat cgagagccgg gaagtcgagg cggacctctc ggaaccttcg
    12781 aaacctgtgg aaccttcggg accggtggaa cccgagggac cttcggagca ggagggggaa
    12841 cttgtcgaat aggagaggac gatgcctgat cccgattccc ggagcaaggg cccgggaccc
    12901 cggggacacg ttcccgtacg aacgtgtgtg gtgtgcggga ccaaaggtgc aaaattcgac
    12961 ctggtgcgtc tggctctggg tcccggcgat gggctcattc gggtggatac ccgtcagcaa
    13021 atcccgggac gcggggccta tgtgtgtgca gggtgccgcc ccggtctgtg cgacaacaaa
    13081 cgcctccgaa gagcatttcg cggcgaggcg aaaggattgg cgggcaatct tgccttcgat
    13141 tcgacgggtg acacggactc tgactcagca aaaccggctg acgacactcc ctgacgactc
    13201 catgggccgg gactcgcgag gtcccgaacc ctccccgttt cccacggctg cggggaaaag
    13261 ggacgcccat ggggccgaaa gggatgtggc cattcgtcca gcaaggggga aggtttcatg
    13321 gggcgtatga gggtgcatga gcttgcaaaa gaactcaata tgaataacaa agacctgatc
    13381 gaccggatac tgaagctcgg gatcgtcgtc aagaaccaca tgagcactct gacggaatct
    13441 gcggttctga agattcgcca gcaattcacc gaaggccggg ccgagaccgt ggaagaaaag
    13501 cgaatcggca gggcgatcat ccgtcggcgc aagaagacga cggccgaaga ggagctgccg
    13561 cccgccgagg ccttggggga aggggtttcc ccggtggaga ccgaggtcgt cccggaagcc
    13621 gcggcggcgc tcgaattgcc cgaggtcccc gcagaagccg ccgaggtcct cgaggccgaa
    13681 gctccggtcg agccgggacc ggaaattgcg gcgatcccgg ccgaagtcga gatggaggcc
    13741 cgccccgtcg tcgtgccgcc cccgccgccc ccgcccctcg agccgggacc cccggctgaa
    13801 gagattgcga ccgttgaact gccggcgccc cgggaaccgg aaggtcccgc agtcgtcgaa
    13861 ccgcccgcgg cggttccgga agaaccgcag caggaggccg ctccggtcgt tccggaggtt
    13921 gtcgcgccgg aggccccggt gacggcgcaa atccatgaag ttcctctgcc gagggtcgaa
    13981 gacggggaac ctgccgccga agccgaggaa gaggcggaag gcgcggcaaa gcccaagaag
    14041 gccaagaaga gacgacggaa aaaggtccgc aaggacgagc ccgcccggat catcaaactt
    14101 cccgaggccg ttcccgagga gccggaggac gaagtggaac tgctgcccat cgtgtcgcgc
    14161 ctccacgtca agaccgagga ccgggaggtc aaggaagccc cgcgcaagaa gcgcactcgc
    14221 ccggaggaag cggaaaagga agcggccgag tttcggaagc gagccggggt ccgcaaggaa
    14281 gtggtcgagc gggaagatct ctacagcaaa aaggagctgg ccgcccaggc ggaccgaggg
    14341 cggttcagag accggggaag gttcgttcgg gaacctgcag aaccggaagc ggccgctccc
    14401 aaacccatca agaggcgcgt ccgaatggat gaggccatca ccatcgcaaa ccttgccaag
    14461 caaatgggcg tgaaagccgc tgaggtcatc aagaagctcc ttgtcctcgg ccatcccgca
    14521 agcatcaacc aggcgctgga ctacgaaacg gcagcccttc tggcctcgga attcgacgcc
    14581 gaggtcgaaa aggtgggatt tgaagaggag gagattctgc aggtgcaaac ggatcgtccc
    14641 gaggaacttc tgttgcgccc tcccgtggtg acggtgatgg ggcacgtgga tcacggaaag
    14701 acctcactcc tggatgccat tcgtcacacg gacgtcatcg ccgaagaagc gggcggaatc
    14761 acgcagcaca tcggcgctta ctacgtcaag ctggaccatg gggaggtcgt gttcctggac
    14821 acccccggtc atgaggcctt taccgccatg cgggcacgcg gcgccaaggt gaccgacctg
    14881 gtcatcctgg tggtggccgc cgatgacggg gtcatgcagc agaccgtcga ggcgattcac
    14941 cacgccaagg ccgccgaggt gcccatcatc gtggccatca acaaggtgga caagcccaac
    15001 gccaacgtgg aacgggtccg gcgggaactg gcggaccacg gcctcctggc cgaggagtgg
    15061 ggcggagacg tcaccatggt cgagatctcg gccaagaagc gcctggggat cgaagagctg
    15121 ctcgagatgg tgctgctcca ggcggagctc atggagctca aggccaatcc ccacaaaccg
    15181 gcccgagggc gtgtcatcga ggccaagctg gacaagggcc ggggacccgt ggccactatt
    15241 ttgatccagg aaggaactct ccgggccggg gacgtgtacg tttgcggaac ccactcggga
    15301 cgggtgcgca acatgttcaa cgaccgggga gtgcgcctcg agtcggcggg cccctccgta
    15361 cccgtcgagg tcctgggctt ctcaggggtc cccaatgccg gagacgactt catcgtgctg
    15421 gcggatgaaa agcaggccaa gctggtggcc gagcatcggc tggtcaaagt ccgggaaaag
    15481 gagctttctc gaaccaccaa ggtcaccctc gagagcctct tcgaacagat ccaggaaggg
    15541 gagatcaagg agctcaacgt catcgtcaag acggacgtcc agggctccct ggaagccatc
    15601 aacgaatcct tgagcaaact ttccaccgcg gcggtgaagg tcaacctcat ccactccgca
    15661 acgggagcca tcaccgaaac ggacgtcatg ctggcttcgg catccaacgc catcgtcatc
    15721 ggcttcaacc ttcgggcgga tgccaaggtg caggagttgg ccgaacagga gcgggtggat
    15781 ctgcgctact acgacgtgat ctaccagctg ctcaacgaca tcaaggacgc catggtgggg
    15841 atgctcgagc ccgtctacca ggagcacgtc ctcggaaggg ccgaggtgag acagaccttt
    15901 cacgtgcccc ggttcggcac catcgcaggc tcctacatgc tggacggtcg ggtggaaaga
    15961 ggggctcggg tgcggctgtt gcgggaccag gtggttgtct acgacggcaa aatctcgtct
    16021 ctgcggcgat ttaaggacga tgtgaaggaa gtgaaggcgg ggttcgagtg cgggatcggc
    16081 atcgagaact tcaacgacat caaggtcgga gacgtgttgg aattcttcga attgcaggaa
    16141 gtcaaaccca cgttggagca cgagcccgag aaacggaccg gggcctaacg gttcgcccag
    16201 gaggcgttga ccatgaccgt cggggtcgca cggatcacct tcagactgca cgggaaccag
    16261 tctctcaagg ataagagaaa gatcgtgaag ggcctggtgg aaaagagccg ccatcggttc
    16321 aacgtgtccg ttgccgaggt ggcggaccag gaccttcatc aaagggcaac cctgggagtc
    16381 gccgttgtgg gcagtgatca tcgggtgttg aattccctgc tggatcgaat cgtagccttc
    16441 atggaagccg tgggctccgc cgacctggtg gaccatgaaa tcgaattgat ccccctcggg
    16501 gactgaaggt gagggcccat gacggaattc aaacgatcgg atcgagtggc ggaccttgtg
    16561 aaacaggaaa tcgccgacct tctgctgcgc agggtgaagg acccccgggt ctcccacgtg
    16621 acggtcaccg gagtcaaggt gagtgcggac ctccagcatg cccgggtcta ctacagcgtg
    16681 ttcccggcga cgtccgaagt ggacaaagag tcggccggcg ccggtttgag caaggcgagg
    16741 ggattcattc gccaggaatt ggcccgccgt ctccacatga gggtcgtccc gcagctcagt
    16801 ttcgagtatg acacgtcttt ggagtacgga gaccggatcg agcggttgat cagagagctg
    16861 cacaaggatg aatgaacctt cggacaagga aatacagaga attgccgaac tgattcatcg
    16921 gcaccgcagc ttcctggtct cgacccatgt gcgaccggac ggggatgccg taggggccct
    16981 tctggccctt gtcttcatgc tccgccggct gggaaagagg gccgacccct attgccagga
    17041 cccttctccg gcctgttacg agtttctggc gggggcggag accatttcca cggagcttcc
    17101 ccggcccggg gtctatgagg tggcggtcct ggtggactgc ggcgacttgg agcgagtggg
    17161 accggccctg gccccggcga ttcgaaccgt tcctttgacc atcaatatcg accatcactc
    17221 gaaaaacgac acccccttcg gcaccgtctc ctgggtcaaa cccggtgcca gcagtacctg
    17281 cgagatgctc tacgacctct cgccggcctt gccggtttcc ctggacgacg aaatcgcaac
    17341 ccatttgtac acggggttga tgaccgatac ggggtccttt cggttttcca acaccacccg
    17401 ccgggtcctg gagatcgcga cggccctggt ggccgccgga gcggacccgg cccggattgc
    17461 ccaagaggtt tacgactcgg ccagtcccca ggggttgaag ctgttggccc aggtgctctc
    17521 ctcggtggtg tttttgtcgg gagaccgact ggcgaccatg gttctcaccc agaggatgtt
    17581 ccaggaaacg gggaccactc ccgaggatac cgaagggttc atcaactaca ttcgttcggt
    17641 aaagccggtg cacctggcca tgctgttccg ccaggaaaga aacggaatga tccatgtgag
    17701 catgcgttcc aagggacggg tggacgtagc cgccctggct cgacggtacg gcgggggagg
    17761 tcaccgacac gctgcggcgt tccgggttgc cgggagcctc gaccgcgtga gggaaacctt
    17821 caccgacgcg gccctggaat atctggacgg gatcgaggga tagggcgtgg agattcttcc
    17881 ggaaaattgc ggcggacgaa ggacgggggg acccatggac cgcccggctt cgccaccccg
    17941 gcccatttcg acggcgcagg aggtggacgg cgttttggtg gtggacaagc cggaagggct
    18001 cacttccttt gccgtcgtat ccatccttcg aagggacctg ggagtcggaa aggtgggaca
    18061 ttgcgggacc ctggaccctt ttgcgacggg agtcctcgtc ttgtgtctca accaggccac
    18121 ccgcatcgcc gaattgctgg cgacccagga caagcgctac gagtttcggg tccgcttcgg
    18181 ggccgagacg gacaccctgg acaagacggg ccgggtggtg agcacctacg cgggacctcc
    18241 cgtaccccgg gggcgcgtgg aagaggccct ggaggggttt gccgggggct acctccagga
    18301 ggcgccaagg ttttccgccg ttcacgtccg gggaaaacgc ctctaccact atgcccgcaa
    18361 gggaattttc gtggaaccgc ccaaaaggga agtgaagatc catgagctgg ccctcaggga
    18421 tttttcctgg ccggaagccc tcctcgaggt tttctgttcc aaggggacct atgtgcgcca
    18481 actggcttcc gacatcggcc gcgttctggg ctgcggggcc gtggtcgccc agctgaggcg
    18541 gctggcgagc ggacccttcg ggacggagga ctgtatccgc atcgaggagc tgaagaaagg
    18601 cgcccgggac ggctcctggc aaagggcggt cctgcccatg aaccgggtcc tggtccaact
    18661 ggagtccttg gtgatccggg aggaggaggt gcttcgcgcc ctgtcctgcg gtcacctgga
    18721 cccttcctgg gaagcccgca tgaagacgcg tgtcgcccaa tccgccggtc ccgtccgcct
    18781 cctgacgggg acgggccgcc tggccgccct ctggtggccc gctcacggcg ccgccgggag
    18841 gtcccggctg caggtatttc ggttcgaggt gtgaaccgtt caaatcgggg atgctggaaa
    18901 ttcgccaaga aacgttgtct ctacacccat tcgggaagtg ggtgtctcaa caagttcgtc
    18961 aacaaggagg aattagccgt ggtaatgacc cctgacagga agaaggaaat tatcgaaaac
    19021 ttcaagatgc atccttcgga tacaggttcc ccggaggtgc agattgccct cttgagcgag
    19081 aggatcaatt acttgacgga ccatttcaaa cttcacaaga aagaccatca ttcccgacga
    19141 ggtcttttga aactggtcgg acaacgacga cagctgctca actatctcaa gaggaagaac
    19201 gtcgatcgtt acaacaccgt catcgaacga ctcggccttc gacgctagac tcggcaggtc
    19261 tgcttccgaa cgccgtcgca ggatggccaa ccaagcatct aatggagacg gaattcactt
    19321 atgaaatatt cagtgcaagt agacgtggga gggcggcccc tgaccatcga atgcggtcat
    19381 atggcccgcc aggcaagcgg ttcggtcctg atgcgctacg gggaaacagt ggtccttgtt
    19441 tccgcaacca aggaagatcg tgttcgggag gggatcgact ttgtgcccct gatggtcgac
    19501 taccaggaaa tgagctacgc cgtcgggagg attcccgggg ggttctttcg tcgggagatc
    19561 ggccgtccca gcgagaagga aacccttacc tcccgtttga tcgatcgacc gatccgccca
    19621 ctgtttccca aaggatactc tttcgagacc caggtaatcg ccacggtgct ttcggtggac
    19681 ctggccaatg agccggacgt gatcgcgttg agcggggcgt cggcggccct tcacatctcc
    19741 aacattccgt ttcaggggcc catcgccgcc gtgcgcgtcg ggagggtgaa cggggagctg
    19801 atcatcaacc ccagcctgaa acagctcgac gaaagcgata tcaacctcgt ggtggcgggc
    19861 agccgagatg cggtggtcat ggtggagggg ggggccgatt tcgtctccga agcggacatg
    19921 gtggaagcca tcgacttcgg ccaccgggcg cttcttcccg tcctggacct ccaggaggag
    19981 ctcaggctca tggtccagcc ggaaaaacag gtttttcagg gaatccaaaa ggaccccgcc
    20041 ctgaccgagg ccgtcgagcg aacggcggcc gaagagatgc tcgccgtcat gacgacggcc
    20101 gacaagcagt tgcgcaaaga gcgcaagaag attctgcgcc agcgtgtctt cgacgaaatc
    20161 ctgatcgaat acccggaccg ggaaaaggag gtcgagggga ttctcgaaga gatcgagaag
    20221 cgggtggtgc gtcgcatgat ggtcgagcag aagcatcgca tcgacggtcg acgcttcgac
    20281 gaaattcgcc ccattaccat cgaggcgggg ctccttcccc gcactcacgg ctccgccatg
    20341 ttccgccggg gagaaaccca ggtgctggcg gtggtgaccc tggggtcttc ttccgacgaa
    20401 caaaaaatcg aagccctctc gggggagacc ttcaagtctt tcatgctgca ctacaacttt
    20461 ccgccctact ccgtggggga agttcgaccc ctgcggggcc cgggacggcg cgagatcggg
    20521 cacggggccc tggccgaacg tgccgtgaag gcggtgctgc cccgggacgg ggaattcccc
    20581 tacaccattc gagtggtctc cgaggtcctc gaatccaatg gatccagttc catggccacc
    20641 gtatgcgggg cctccctggc cctcatggac gccggggtcc ccattcggga agccgtggcg
    20701 ggaattgcca tgggcctgat caaggaaggg gaggaattca tcattctctc ggacatcctg
    20761 ggggatgagg accacctggg agacatggat ttcaaggtga cggggtccga gaacggcatt
    20821 acggccctgc agatggatat caagatttcc ggcatcaacc gcgccatcct ggaacaggca
    20881 ttggaacagg caaggatcgg acgcctcttc atcctcaaca aaatgaagga aatcctctcc
    20941 tcgccccgtc ccgagctctc cccgtttgct ccccgggtgg tgtccatcat gatcaatccc
    21001 gagaagattc gcgacgtcat cgggccggga ggcaaggtca ttcgctccat tgtggcggaa
    21061 accggggcca agatcgacat cgatgattcc ggccgcgtgg tggtgatgag cccggacgcc
    21121 caggcctgtg agaaggccat cgaccgaatc aagagactga ctcaggaagc ggaagtcggt
    21181 caaacctaca tggcccgcgt ggttcgcgtc accgattttg gggccttcgc cgagatcctg
    21241 cccaacatcg aggggctcat ccatatctcc cagctcgagc acaaaagggt gcgaaaggtc
    21301 tccgacgtgg tcaacgaggg agacgaagtg ctggtgaagg tgatcgacat cgataaggac
    21361 ggccgcatac gactgagccg caaggccgtg ttggaaaagc ccggcgagac tcgatagtgg
    21421 ccgggacccg cctcgaaggg ggcggtcctc ttggtggcgt gggagcgggc accgagggcc
    21481 gggtttcccc ggcactcggg aacggggacg gagcactcac ccccgggggc cgtacggtgc
    21541 cgccgtgacg gctcgaggcg gtttgaaatc gttttctcag acaggaacgg gtccgtggag
    21601 gggggcggtc ggccaccttt cggccccaac gggaatgagg ggaacttgta tcagaagacg
    21661 gtccttcaga acggtattcg ggtcgtaacc gagaagattc cttacgtcca ttcggtttcc
    21721 acggggattt gggtttgtgc cggatctaga gacgaggggc aacaagagcg gggaatcagc
    21781 cacttcatcg aacacatgct cttcaagggc acccagcgcc gcagcgccct ggacatcgcc
    21841 aaggaactgg atgcggtcgg cgggtttgcc aatgcgttca cgtccaagga acacgtatgc
    21901 ttccacgcca aggtgctgga cgcccatttg cccctggtgg tggacgtcct gaccgacata
    21961 tttctcaatt ccgtcttcgc gccccaggaa atcgagcggg aacagcaggt aatcctgcaa
    22021 gagatccgca tgatcgaaga caccccggat gagtatgtcc acatcctttt ccaggagcgg
    22081 ctctggaagg acaatcccct gggcctgccc atctacggca gtgtggacac ggtcgagggg
    22141 atcgaccggg aaagcatcct gcgctacgtt tccacgacct ttgaccccaa gcgcattgtc
    22201 attgccgccg cgggaaatct ggagcaccag cggttcatcg acctggtggc cccttccatg
    22261 gaacggctcc ggccctcctc gaaccggatc cgccgcgaga cccccaacag ccatcacctg
    22321 gtccacgtga tttcccggga cctcgaacag gtgcacctgt gcctgggcat gaaaggcacc
    22381 tcccaggtgg atgaacgccg gttcgcatgc cacctgctca acgtgatcct gggcagcagc
    22441 atgagcagcc gcctgttcca ggagatccgg gaaaggcggg gactggccta ctcggtctat
    22501 tccttcacca acagccacga agacacgggc ctcatgggcg tgtacgcagg ggtcagcccc
    22561 gagaacgtcc aggagaccct ggacgtcatc ctcgatcaac tggccctgct gaccagggac
    22621 cccattcctc cccaggagct cgcggcctcc aaggagtacc tcaagggcag catgtacctc
    22681 aacgccgaga gcaccgatgc gcgaatgaac cgtctggcca aaaacgaatt cctcttcggt
    22741 cgtcaagtct ccttcgagga ggtcgaacag aagatctacg aggtgagcgc cgaggaggtg
    22801 caggcctggt tccagaccgt ctgccgaccg ggggaattca cggcggtcct tctgggaccc
    22861 gtggaccccg aatccatccg gctgggcccg gtcgttgcga ccaacggctc ccaagtctga
    22921 ggcaggttct tcatggccct ttgctttcag ctgatcgcca gcgggtccaa gggaaacgcc
    22981 atcctggtat gcagccccaa gacccggatc ctggtggatg ccggcctgag cgcccgggaa
    23041 ctgtgcaagc ggctcgagaa aacctgcact cccgcgaatc gcctggatgc cctggtcctc
    23101 agccacgaac acggcgacca tgttcgaggg gcgggggtcc tcagtcgccg tttccatgtg
    23161 ccggtgtacc tgaaccaggg cacccgggag aacctgccgg agcagacggg cccaatggag
    23221 tccatgcaga tctttcagtc cgggtctcct ttttccgtgg gcgacctgga aatacacccc
    23281 ttttccattt cccacgacgc agcggacccc gtggggttcg tggtcgagca caacgccgtg
    23341 cgattgggaa tctgcacgga cctgggggtg gccacccagc tcgtgcgaac tcggctccgg
    23401 gggtgccgag gcatcgtcct ggaagccaac cacgacacgg accttctgct caacggccct
    23461 tatccctggg agctcaagca gcgaatccga gggcgccacg gtcacctgtc caactcggat
    23521 gcctacgaac tcctcgacac ccttcaccat gaccggctga aaaccgtcgt gttcgctcat
    23581 ctcagtgaag tcaacaacca ccccgatctt gtccgtacgg gctccaatcg cttcttctgc
    23641 cgggactcat ggcgggacgt cgattttgta attggaaaac aacacgaaat ctcaggagaa
    23701 atggaacttg cctgagcctt tgccccatgg agggacctgg aggcgagaat ggaatccatc
    23761 cccaaatgtt tgcttttgca gatcgatgcc accgaggaat ccttgaggcc ggtgaggttc
    23821 ctggggcgtc tttatcccga tgcggcgcaa atggagctga tcctctccta ctttcagccc
    23881 cccttgcccc ccgtctacaa gcaatacccc ctgcccccct ccctggtcga gaagagaaag
    23941 gaactcctcc ggtcccggga gacccaaacc cgctcgatcc tcgaccgggc caggaccgtt
    24001 ctcaaggagg cggggttttc cgaagacaag atccaggaac acgtgcagga acgggaatcc
    24061 agtgtggcac ggcatgcgtg ttccctggcg gaccgcaaaa aggtggacgc ggtgctggtg
    24121 cagagacatg tctgccggga cctcgaggac ttcctcaagg acgatcccac cgatgcgctg
    24181 ttgcatcatt gccaggtaag tcccgtctgg atcgcggacg gggagatcga cccgggtcac
    24241 gccgccgtct gcattcagaa tgaagacgcg tctctgcggg cggtggacca tgccgccttc
    24301 atgctcgcgg aatcccccgg ccaaatcacc attctccatg tctcccggtc ggtttcgcgc
    24361 cccatagaaa gcggactgac ggactacggt tccgaattcc tcggatggct caactccccc
    24421 ggcggcactc ccctgagatc ctttttcgac aacagccgtc ggattctcca ggatgccaag
    24481 atccaggagg accgagtccg gttggcggtc catcccgtgt catggaacgt ggccgacacc
    24541 attctcggat actgccggga acgccgcgtg ggggtgaccg tcctgggcca ctcgggagaa
    24601 cagggcattc tggggttcct gagaggatcg gtcaccaaga ggatcctgtc ggagttcaag
    24661 aacatggccg tatgggtgac ccagtgacgg gtccggccgg tttccggggt tgcccacgtg
    24721 tcccgggttc accgaggccc gactcgcccg ttccttgctc gaaacggggt tttttccacg
    24781 gtcaattcgc cgcgttcgga tatcggataa aagtatgata ggatctaaga aggacccagc
    24841 gttgcaccga gacgatctat agaccggggg ggacatggag aagaagaagc cggtcgttct
    24901 cgtggccgag gacaaccttg tgaaccagca gttggccaaa aggatgctcg aggtgctggg
    24961 gtgcgaggtg gaccttgcag ccaatggaat cgaggccctc aacgcgttcg aaaccggctc
    25021 gtatgatctc gttctcatgg actgccagat gccggagatg gacggtttgc aagcttccat
    25081 ggccatacgg gagcgggaga aggcctcgga ttctcgatat cccgaggggg ccctcgtgtc
    25141 ccgtgccgtt cccatcattg cggtcacggg gcaggtttcg gaccatgatc gagacagctg
    25201 cattgccgcg ggcatgaatg attttctcag caaaccgttc cggctgcacg aggtcgaagc
    25261 catgctgaaa aaatggcttc acgatagaag aaaacacgac tagactcttt accgcttcat
    25321 cgattctttc ctccaaaccg agtctaaagc ccagagctga gttggatgcg ccatgagacc
    25381 ccactcgtac gggagattct ggatcctggg agccgtaacc ctttggctcc tatctcaccc
    25441 cgtcccggca actcctcttg aggattcccc ccagcccccc gctcccaccg aggaggagtg
    25501 gatgctcttc caagagatcc cgtccgtcta cggggcttcc cggtatgagc aaaaggtgac
    25561 cgaggctccc tcctccgtga gcatcgtgac ctctgcggac atcaagcatt acggctaccg
    25621 aaacctcggg gagatcctgg aaagtgtgcc cggcctttac accacctacg accgcaacta
    25681 ctcctacctg ggcatgcgcg gctttgcaag ggccggggac tacaatactc gagtcctcct
    25741 cctggtggac ggacaccggc ttaacgacgc ggtgtacgat caagcgccca tcgggaccga
    25801 ttttcccgtc gatgtggatt tgatcgaccg agtggagatc attcggggtc ccagttcatc
    25861 catttacggg accaacgcgt tcctgggggt catcaacgtc atcaccaagc ggggcagaga
    25921 cctgagaggc tcctaggttt cgggagaata cggaagctac gagacctacc gaggccgcta
    25981 cacctacggg aatcgaaccc gggacggcgt cgaattcctt ctttccggct cctattacga
    26041 cagcctgggg gacgatcact attaccgacc tttcaggcac tcccatcgcg ggatcgcctc
    26101 caactgcgat gacgaacgtt tctacgggct gttttccaag gcatcctacc aggacttcac
    26161 cctcatggga gtcttccact cccgggaaaa aggaattccc acggcaccct gggatgtgat
    26221 tttcaacgac ccatccaccc ggaccacgga ccatcgagcc ttcctggacc tgaaatacga
    26281 ccgcgaattg ggggatcggc tggacctgtc ggcccacctg tattacgacc actaccgtta
    26341 tgacggcgac tatgcctact ggcaggaccc ggaccgaagg tggaaccggg tcctgcagaa
    26401 ggactacgct cggggtgaaa tggcgggggc ggacgttcaa ctgaccaaga ggtggctgga
    26461 gaaccacaaa ctgttgctgg gatccgaata tcgcggcagt ttccgccagg accagggaac
    26521 ctacgacaac cacccgtacc ggttgctcat gaaggagcgc agagacgcgt acaactgggc
    26581 cgtgtacctc caggacgaat accgaatctt ctccaacctc ctgctcaatg cgggggtgcg
    26641 atacgaccaa tacgaaacgt tcggaggaag caccaacccg agactggccc tcatcttcaa
    26701 ccccatcgag aagagcgcgc tgaaattgat ctacggacag gcattccggg cgccgaactc
    26761 ctatgagctc ttctataccg accggatcaa ccagaaggcc aacccggacc tggatccgga
    26821 gaacatgacc acgtatgaac tcgtctggga acaacggctg accgatcgcc tgcggctgtc
    26881 ggctaccggc tactactatg agatcgaaga cctgatcagc ctgacccgaa tgccgaacgg
    26941 tttcctgatg ttcaagaacg tcgaaaacgt gcaggccaag ggtctcgagt tcgagttgga
    27001 cagccggtgg cccggaggga tcgagggtcg tgtgagctac tgtctacaaa ggacggaaga
    27061 cgaaagatcg gggaatgtcc ttcccaattc cccgaaacag atggtcaagc tcaacgtgct
    27121 ttgtcctctc atccaacaca agcttacggc gggcaccgag ctccagtacc tgagccctcg
    27181 tcgtaccttg cgcgacaaca agacccagga cgccttgatc gccaacctca ccttccatgc
    27241 tcccaacgtg gtgaagggac tggatgtctc cgccggtgtc tacaaccttt tggaccagga
    27301 ttacggagat cccggatcgg cggagcacct tcaggacatc attcaacaaa acggcatcaa
    27361 tttccgactc aagatcacgt attccttcta actatgaaaa aaagtcgagc tcgtccgcac
    27421 ccggaatttc gctttcggaa atcagaacct ctcaacggcc ggccgggggc cctcccaaga
    27481 acgagaaacc gtcccccggt tgttcgaaag gtcgaaaagt tgcgccgggt tgcccggggg
    27541 ctcttgggga caacggctgc cctgcttttc cttttctcca tggcggaagc gacctccgaa
    27601 aagaccggaa cggaagtcgc cgttctgctg agtcaccgag tgcagcccta cgaacaggcg
    27661 ctggcaggct ttcagcaaca cctgcgggaa cgggagacct caggaccgat tcaggtccac
    27721 catctgggtg aggaagtggg aagagacgct caagcggcgg attccattgc ccgggagccc
    27781 ccccgggtac tgctcaccgt gggttccccg gcggcgcggc ttgccgccga gcggctgagc
    27841 cacattccca tggtggcggg aatgattctg tcgccggagg atgtgaaagc gcatcccaac
    27901 gccacggcgg tggtgctgga cattcccctg gaggttcaac ttgcgtggct tacccgggtg
    27961 cttcccggtt gcagaaatgt gggagtcctc taccacacgg aatcgaatga aaggaaggtc
    28021 gaaacggccg gggttttggc cgatcgcatg ggactgagac tctacgcgcg caaggtccaa
    28081 tcgccctccg aaatcccgga cgccctgggc tttctatcca agagggcgga cgtgctgtgg
    28141 ggtcttccgg attcgctggt tgtcactcca cagactgcaa agcctttgct gttgttttcc
    28201 ttccgcaaca acatcccctt cgtgggcctg tcgagctcgt ggaccaaggc gggatccctg
    28261 tattccctgg attgcagcta tccggacatc ggtgtgcaat gtgccgaagt tgtcctcaaa
    28321 gttttgcggg ggaccccacc gcgctccatc cctcgggtgc atccccgcaa agtgcttctt
    28381 tcgctgaact tgaagaccgc ccaacatatg aggctccaca tcccggaacc ggtcgtgcgg
    28441 agctccgagc aggtgtttga atagagagga gaaaggtcat gcttggacga tggcggatcg
    28501 gcctggtggg caaattcaac ctgctgacca tttctcttat cttgatcacc tccttgggca
    28561 tcgcctcctt cgtcatccgt caaaccagcg aaaaccagca ccgggctctc attcagcacg
    28621 gaatcaccac ggccgacatc ctctcccaga acagcgaata cgctctctat accgaagacc
    28681 cggagctcat gaagcagatc ctggagagcg tcagcgccat cgagaacatc agctacgcag
    28741 ccctgctcga tgctcgacgt caggtcttgc tggaaacccg gttcacccac tcggtgcagg
    28801 tccctaccca tttgaggcag gtccattcgg gcaatgaaac cctttttgaa gagacgcccg
    28861 aggacaagag cggcaggcgc ttcatcgaca tcgtggcccc cgttctcgtg catcccgagc
    28921 tggacttgga cgtcatgcag gacaaatccc gggagcggga gggtcccaag aagctcggct
    28981 atgtccagtt gggcctggac atggagagac ttcatcgccg gagccgggag tttctgcttt
    29041 ccaccctctt tttcacaacc ctcatcggcc ttgcagggat ctttgtgtcc atcatgttca
    29101 cccggagaat tgcgggaccc gtccgggcgc tggcggccgt cacccgagag atctcggaag
    29161 gaaagcttcg caaaccgatc gaaatcgacg cccgcgacga aatcaaggac ctgaccgact
    29221 ccttcaacgt catgctcgag cggctcaggg aatcccgcga gcaggtggaa gactaccagc
    29281 ggatgctcga ggagaaggtc cgagagagga ccatccaggc tttgcgggca gacgaggcca
    29341 gccgggccaa gtctcaattc cttgcgaaca tgagccatga aattcggact cccatgaacg
    29401 gggtcctggg actggcggag atcctgctgc agggggacct gacggatcgc cagaggaatc
    29461 tcgcggaaac gatccatcat tcgggcatga ttctcttgag gattctggac gacattctgg
    29521 atttttccaa gattgaagcc ggaaggctcg aactggacca tgcaccgttc gatgtcgcgg
    29581 aggcggtcga agatgtggcg gaactttttg cggaacaggc ccagaaaaag aatctggaac
    29641 tggtggtcga cattgcggcc gaccctcccc ccatcgtcca cggcgatcct gctcgtctgc
    29701 ggcagatcct gaccaacctg gtcagcaacg ccatcaagtt taccgagaaa ggggaagtgc
    29761 tggttcgcct gggatgggtg gaatcccggg acgattcggt gcgaatgggg ttcgagattc
    29821 gagacacggg cattggaatt tccgagcagg accaacccag gatcttcgag gtcttcaccc
    29881 aggcggacag ttcgagtacg cgaagacacg gcggaaccgg cctgggcctg tgcatctctc
    29941 ggcagctctg cgagatcatg gggggcaaga tccgggtccg aagcgaggtg ggcaaaggga
    30001 gcacattctt tttcgatgtg gttttcggga cccccggaac cccagtggac cctcggacag
    30061 ggctccttcc tgggcggccc gacccacccc gcgtcctcat cgtcgacgac aacgaaacct
    30121 gccgcagagt cctcctgaac tattgccgcg ggtggaacct ttcagccgaa gccgcatccc
    30181 atggtatgga agccctccga ctgctccgga acgctgcggc ccggggaaaa ggctacgact
    30241 ttcttctctt ggacgcggac ctgggcgaaa aggacggcat cgagctggct ggggccgtga
    30301 cccgtcttct cggggagaat tctccccgcg tccttctggc caccaccctt ggacaacagg
    30361 aacgactgga agaagcctat gaagccgggg tgtccgtctg tcttcgaaaa cccgttcgaa
    30421 agtctcggtt ggccgagaag atcgccgggt tgtggctgcg tggagccccg ggggaagcgc
    30481 ccgcctcgcg ggaagaaacc cgggaaccga ggcgggaaat cctcgccggc gcccgggtcc
    30541 ttcttgccga agacaatgaa atcaatcagg aggtggcccg cctcatgctc gagggcctcg
    30601 ggtgtcgggt ccacaccgtg gacaacggaa tccaggcggt ggatgctgtt ggaagaaatc
    30661 cctacgatct tgtgttcatg gactgccaga tgcccgaact ggacggatat gaggccactc
    30721 ggcgcattcg agattgggag aaggagcggc actcctcggg gtccgggaaa ccgttctcca
    30781 ggatccccat tgtggcgctt accgcccacg ccatggccgg ggatcgggac cagtgtctgg
    30841 cagcgggaat gaatgactat ctgagcaagc ccttcacctt ggatcaactg cgccgggttt
    30901 tgcttcggtg gtaccctgcg ggaggaacgg agcccaatcg ggacgaaccc ccgatggagt
    30961 tctccgccgc accccacaac cctgcggatc gctcctcgcc tcttgacccc ggcgccctcg
    31021 aggccattcg atctttacat cgggaaggag aaccggatat tcttccggac ctgatcgaga
    31081 tcttcaagag ggagtcggcc caattcctcg aaacgatccg acaggccatg gaccgggagg
    31141 acccggagac ggtccgcaag attgcccaca gctggaaatc cagcagcgcc aacttggggg
    31201 cgacccacct ggcgaaattg tgcatggaga tggaacaact gggcaggagc gggcatctcg
    31261 aacccgccgc ccggatcctt acgaaggtgg aggctgagct ccgcctggtt tttgaggcct
    31321 tggacagcga gattccgaaa gatgagtcga cccgcccctc ccagggatga attccccatg
    31381 tcctctttcc tctcccggat cccgctccgg gattttcatc gatccgcctc ccgcaggtcc
    31441 gacccggagc tccttcaaag gaatcccgcc cccaaggaaa tcttgtacct tcccctgtgg
    31501 gacgacctga acctgttttc cccgctggga gacgcccagg cggtggtcct gggaaaggaa
    31561 gcggtgggag ggttgggcgg tgacgtttcc gctgtctcca tctacctggg ggagaccccc
    31621 cggagcgtct tttttgcctt gtgcctatcc gccgaggccg ggttcaaccc cgaggcactc
    31681 tcccggtacg gcagtttcct ggacctgcga aaggccctcc ccgtgctgga ttcagagcag
    31741 ttttccctcc tggccttcgc caaatccctg gcctgctggc aggggcgagc ccttttctgc
    31801 ggagcctgcg gcgccgggac ccgcgccggc catggggggc acgtgaggac ctgctccaat
    31861 gaaacatgcg cccgccagta ctttccgcgc acggacccgg cgatcattgt gctggtcctc
    31921 cggggggaac gctgtttgct gggcaggcag tcgagctggc ttcccgggcg ttattccgtc
    31981 ctcgcaggct ttgtggaacc gggagaagcc ctcgaggagg cggtgatacg ggaggtggaa
    32041 gaggaaaccg gggtgagggt ccgggacgtt cgctaccatt cctcccaacc gtggccgttt
    32101 cccagctctc tcatgatcgg gttcaccgcc cgggccctgg atgaccggat cgatggggtt
    32161 cccggagaac tcgaggacgc acggtggttt tcccggaaag agattgcagg ctccctccgg
    32221 gatggatccc tggtgcttcc tccccgggcc tccattgcaa accggctcat cacggaatgg
    32281 tacgatgccg gggaatcctt gacgttgaac gatctgctgg aatccctgcc tcatcgtcaa
    32341 gcctttcgtt gatcccgcgc accaaaggaa ctggaagcca ggatgcaagg atatctgctc
    32401 aggagactca ttcaaatcat ccccacactc ctgggcatta cgttcattac cttcctgatc
    32461 attcagctgg caccgggaaa tcccgccctt ctgaagctgg agatgaccgg tcagggccag
    32521 atgagcgaca gcgccgtttc ccagcaaatc atcgagcaga cccggcagtt gtacgggctg
    32581 gacaagcccc ttcccgttca atacctgctg tgggtgaaac gggttttcac cctggatttc
    32641 gggacctcct acaaggatca ccgggacgtc tgggacaaga tcctcgagag acttcccgta
    32701 accctgcaac tgaacctggc ctccattttt ctcgtgtacc tgattgcgat tccctgcggc
    32761 gtctattcgg cgacccatgc aggctcgctc acggaccggt ttctgaccct tctctttttc
    32821 ctcctctatt ccctgcccgg cttttgggtg gccgtcttgt tgatcatgct cctgggagga
    32881 ggagactttt gggattggtt cccggtgtac gggatctcgt ccattggagc ggaatcctgg
    32941 tccttttccc gttggctcca ggaccggctg tggcacctgg tcctgcccgt ggtctgccag
    33001 acctacgggg gactggccta cctttcccgc ctcactcggg cggaaatgct cgaggtgatc
    33061 cgggaagatt atgttcgtac ggcgcgggcc aaggggctcc gggaacgggt ggtcatcttc
    33121 aagcacgctt ttcgaaacgc cctgctgccc atcgtgacgc tcctggcgtt cctcctgccc
    33181 tccatgttcg gaggcagcgt catcatcgag agcattttct ccattccggg gatgggacag
    33241 ttggggttcg aggcggtgtt gagccgggat taccccgtga tcatggccat cacgaccatt
    33301 tccgccctgc tgaccctggc gggtttgctg atctcggacc ttctctacgc cgtcatggat
    33361 ccgcgaatca agttggaatg aggaaaaagg ggggaaagca tccatgagac cgggagaccg
    33421 gccggcaatg aagttgccgc gatcctatcg gcagatggtc tggcaccatt tcaaaaagaa
    33481 aaggctttcg gtctggggcc tccgggtcac ccttctcctc tttgccgtcg ctcttttcgc
    33541 tccgttactg gccaacgatc gtcccatcgt cttttaccgg gaaggccggt ggtcctggcc
    33601 cgttttctcc gggcaagaca aggtcggttc cctgacctgg aaagagatca ggaaaggggc
    33661 cgatttcggc acccatccgg gagggagctt cgagaagaac tgggtggcgg tctggccccc
    33721 ggtgccgtat tcccccacgg aatacgacct ggtggagagc cttgcgcctc ccggtcgcga
    33781 ccactggttc ggcaccgacg acagtgggcg ggacgtcttg agccgcatga ttcacggggc
    33841 gaggatttct ctttccgtgg gctttgtggc ggtgggcatc gccctggtca tcggcatctt
    33901 cctgggggcc ctggccgggt acttcggagg ctggaccgat cacctcatca gtcgaatgat
    33961 cgaaatcctg ctcaccattc cgactttttt cctcatcatt gccatcatcg ccttcctgcc
    34021 gcccagcatc tacaacatca tgatcgtcat cgggcttacg ggttggagcg gcgtggcgag
    34081 atttgttcga gccgagttcc tcaagatcaa gcaactggat ttcatccagg cctcgaggtc
    34141 cctgggggct tcccatttgc ggatcatctt cgttcacatg ttgcccaatt ccatggcccc
    34201 ggttctcgtc tcggcggtgt tcggcatcgc cggagccatc ctcacggaat cgagcttgag
    34261 ctttctgggg ttcggggtgc cgcccccgac cccgagctgg ggagacatcc tgtcccagag
    34321 ccgcgattac atcgaatttg cctggtggtt gaccgttttc ccggggctgg cgattttcct
    34381 tagtattacc gcctataatc tggtaggaga agggctgcgg gacgcaatgg acccgaaact
    34441 gtttcggtaa aaccggcggc gagaaccgac ccggcggtcc tcgagagcga agggacggca
    34501 aaaggcccgg gccgatcccg accgcggccc ccggcatgtc gggtttcccc ggatgaaaga
    34561 agaccatgcg gcagcaggca cttgcagaga cccacgttta caccgtaagc gagctcaacg
    34621 ccgaaattcg ggaccttctc gaatcccggt acccgttcgt gtgggtgacg ggagagatct
    34681 ccaatttccg ggtcccctcc tcggggcact tctatttcac cctcaaggac gaacagagcc
    34741 aaatccgggg ggtgtttttt cgctctcaga accgctacct gcgctttgtt ccggaatcgg
    34801 gcatccaggt tgtgtgccgg ggtcgcctgg gggtgtacga accccgcggg gaataccagt
    34861 tcatcgtgga cgtcatggaa ccccggggct tgggggccct tcaactggcc ttcgaacagc
    34921 tcaagaagaa gctggggtcc gaagggctct tcgacgcttc ccgaaaaaag cccatgcccc
    34981 tttgtccccg gcgcgtcgcc gtggtgacgt cctctacggg agcagccgtc cgggatatcc
    35041 tcaaggtact cgagcgcagc ccctatcccc tcgagatcac gatcctgccc gtgcgggtcc
    35101 aggggcaggg agcggccgag gaaatcgccg gggcaatccg caaagcgggc gaactgcgga
    35161 atgaattcgg ctgggacgtc cttctggtgg ggcgaggagg gggcagtatc gaggacctgt
    35221 gggcgttcaa cgaggaagcg gccgcacggg ccctggcttc ctgccccatc cccaccatct
    35281 cggccgttgg ccacgagatc gactacacca tttccgactt tgtggcggac ctgcgggttc
    35341 ccacccccac cgccggggcc cagtgggtgc tccgtcgaat ggaggaattt caaagggagc
    35401 tggcccacgg aacggaccgg atggtcgagg ttctccggcg caaaatcgag gggtcccggc
    35461 aggccgtcga gatgctggaa aaacgtttgg tcagtccccg gcgccgtctg gaagactgga
    35521 agctgttcct ggacgaccgc ctggaacgca tgcaccttgc catggctcga cgcatggaac
    35581 gccagcagac gagcctggcg cacctggtgg accgtctgcg atcccgtcac ccttcgacga
    35641 ccatccaaca gtcccggatc gaactgtgcc gtctgcagag ggaaatggcc ttccagcttt
    35701 cgaaaaatct ggaagcccgg aaataccggt tgcaggaata tgcatcgagg ctggatagcc
    35761 tcagtcccct ggcggtctta cagcggggat acgccatcgg gtatcgactc gaggacatgg
    35821 cggtgcttcg aaactccggc caggtttgcc cgggcgacga gatcgggctg aggctctccc
    35881 aaggagccct gaaatgcact gtgcaggggg aatgggagac aaccccccta tcttgaagga
    35941 tgaaggatgt cgagaaagaa aagcgaaccg ttcgaggacg ctctgaagaa gcttcaagcg
    36001 gtggtcgaga agctggagcg gggggacctg cccctggagg aagccgtcga gtctttcacc
    36061 gaagggatcc gactggtgca gttctgtcac aggaagctcg aggaagcgga gaagaaggtc
    36121 caggtcctca tggcggaccc cgagggaggg gtgagcgccc ttccctttga agcggaatcc
    36181 tcccaggatt gagacatggg gcggatgacg cctcaaaaca gtggaaaggc atgggaaggc
    36241 gtggtagagt taggtttgta accccgggac caagaatcga acgctgatgc aaccctttga
    36301 tctgaagaag taccttaccg aacgaaaaga atgggtggaa accgccctcg aacaatactt
    36361 cccaccttct tcgcgcctgg aaaagcgggt cgtggaagcc gcacgataca gcctctttgc
    36421 cgggggcaaa cgccttcggc ccattctttg cctggccgcc gccgaggccg tttcggggac
    36481 ccgtcaatgc gcggcgtccg ccgagatgct gggaagttct ttccaggcgg tcatgccggt
    36541 ggcgtgcgcc atggagatga tccacaccta ttccctgatc cacgacgacc tcccagccat
    36601 ggacaacgac gattttcgca gaggtcgccc cacgaaccac aaggtttacg gagaagccgt
    36661 cgccatactg gccggagatg cgctcctcac cgaggccttc gggatcctgg cccgattgcg
    36721 caccaacggg atgtttcctg ccgagaggat cctccgggtg gtggacatca tcgccgaagc
    36781 cgccggggcg cggggcatga tcgggggaca aaccgtcgac ctcgagtccg agaatcggga
    36841 agtggacatg gacaccgtgg tctacatgca ccggtgcaag acgggtgccc tgattacggc
    36901 ttccctcgag gcaggggcgg tcctcgtggg aggggaagaa gaccgcgtgg ccgacctgac
    36961 tcgatacggg catcacctgg ggatggcctt ccagatcacc gacgacctgc tggacgtgga
    37021 aggggatgcc cgtctcatgg gcaagaaccc gggatccgac gccgccaaga acaagaagac
    37081 ctaccctgcc ctcttgggga tcgaagcctc ccgggagtcg gccaaacacc atgtggagga
    37141 agccctccgg accctgtccc ggttcgatgc atcggccgag cccctgaggg ccgtcgccaa
    37201 acatctgctc gtgcgaaagg ggtgagagga agcggggtgc gtcgtggttc cggacgttgc
    37261 ccgcaaacgt gggagtgatc gctcttttta aaagaggttg gaccttgatg gaagaaccgc
    37321 agaaatcgtt gctttcccgg attgacagcc cttcccaact caaacaactg actctcgctc
    37381 aactgcagca actcgcccag gaaattcgcg aaacgatcat tcagacccta tcggtcaacg
    37441 ggggccacct ggctcccagc ctgggagtgg tcgagctcac cctggctctc catttcgttt
    37501 ttgacagtcc tcatgaccgg atcatctggg atgtggggca ccaggcgtat gcgcacaaga
    37561 tcctcacggg acgccgttcc cagttccata ccatccgcca gtttggaggg atcagcggct
    37621 ttcccaagag aaacgagagc aggcacgacg tcttcgacac ggggcactcg agcacctcca
    37681 tttccggggc cctgggaatg accgtagcca actccctcaa agggtcccga aaccgaacca
    37741 ttgccgtcat cggagacggc agcatgaccg gcggcatggc cttcgaggcc ctcaaccatg
    37801 cgggagacct ggacaaggac ctcatggtcg tcctaaacga caacgaaatg tccatctccc
    37861 ccaacgtggg tgccctttcg tccttcctca gccgaaagct ctccaacaag accctgctca
    37921 acctcaagcg ggagatggag agttttctca agtccattcc cgggctgggt cccaacatcg
    37981 ttcagctgct ccgaagaagc gaggattccc tcgtcacctt cttcaccccc ggcatgctct
    38041 ttcaggccct ggattttcat tacatcgggc ccatcaaggg tcatcgcctg gatcgcctgg
    38101 tggaggtcct ccaggccgca cggcagatca aaggtcccgt gctcgtccac gtcatgaccc
    38161 agaagggcaa gggatatccc cccgcagaaa aagaccccac cagctttcac ggggtggggg
    38221 cctttgaggt ggaaacgggc aggagcctca atgccaaggg gggcgccccg tcctataccc
    38281 acgtctttgg ggaaaccatc acgagcctgg ccgagcggga taccaaggtc atcgccatta
    38341 ccgcggccat gctccagggc acgggcctcg agctcttctc caagactttt ccggaccgct
    38401 tcttcgatgt ggggattgcc gagcagcacg cggtcacctt cgccgccgga ctggccgtag
    38461 acggtttcaa gcccgtggtg gccgtctact ccaccttcct gcagcgggct tacgaccaga
    38521 tcatccacga cgtatgcctc cagaaccttc ccgtggtatt cagcatggac cggggaggtg
    38581 tggtgggcga ggacggcccc acccaccacg gcgttttcga cctctccttt ctccgccaca
    38641 ttcccaacat ggtgctcatg gctcccaagg acgaaaacga gctgcgacac atgttgaaga
    38701 cggccctgga tcacccgggg cccgccgccc ttcgctatcc ccggggaaat gggatgggag
    38761 tgcccctgga cgagaatttg caagccctgc ccatcggcag gggcgaagtc ctgcgcgagg
    38821 gacccgatat cctgctgatc ggcatcggct ccaccgtcta tccctgcctc gaagctgcct
    38881 cgaagctcga aggacagggg atcgaggcca cggtcgtcaa cgcccggttc gtgaaacctc
    38941 tggaccggga gctcctcctg gggcttgccg agaagatcgg ccgggtagtc accgtggaag
    39001 agaacgtcct ggcgggagga ttcggaagtg cggtcctgga gatgtttcag gaagccggtt
    39061 tcttccccgg ggacttcgtg cgcctgggat tgcccgactc cttcattccc cacgggagcc
    39121 agagcatcct gaggagcatg cacggcatcg acgccgacgg catccaaaag gccgccttgg
    39181 ggatcctgca agcccgccat ggccaggtgt tgcgtgcgat tggacaggtt gctcgtcgat
    39241 aaaggactgg tatcgagtcg ggagcgtgcc caggccctga tccttgcggc ccgggtccgg
    39301 gtgaacgggg ttcctgcaac caaggcggga caaagcgttc gcgaaggttc ccaaatcgag
    39361 ctgttggggg atctcccctt cgtgagccgg ggcggctaca agctcgatca tgccctggac
    39421 caattccacg tggacgtgcg gggccttacg gccatggacg tgggcgcatc cacgggaggc
    39481 ttcaccgatt gtctcctcca gcggggtgcc cgccgcgtct acgccgtgga cgtaggatac
    39541 ggccaactgg catggaagct tcggcaggat gagcgagtgg tcgtcctcga acggcaaaac
    39601 attcgtcatc tctccacgga cctggttgcc gagcccattt ggatttgcac catcgacgcc
    39661 tccttcattt ccctcagacg ggtcattccc gccgccctgc gcttcctggt tcccggagcc
    39721 cggctcatcg ccctgatcaa gccccagttt gaagccggcc cttcccgggt cgggaaaagg
    39781 ggagtggtgc gggaccccgc cgtccacgac gaaatttgca cctccctgca acgattcgtc
    39841 caagacctgg gattcgaggt gctgggcatt gtgccctcgc ccattctggg tcccaaaggc
    39901 aaccgggagt tcctcctggg ggccgttttt ccgagagcct agagggtctc ccgaaagctt
    39961 ccgccacgcc gttcacacgc cgggtccgac ttcccggagc ctaccccgac ccaagaaccc
    40021 cttttccttt ttccacgatc caagggcccc gacgaatccg cccgatttcc caccgccctg
    40081 cgactgatac caagttgcgc ccaagatcgc cctgacatgc tgagtgaaga tttcgagcaa
    40141 agggatgaat cctacgggtg ggcagtttcg gcttaaatgc aacttcgtat gagagctgtg
    40201 cgggatctcc cgtcacggga gaccccaaac acattcttcc ccaacggggg aaatagccgc
    40261 cggttgtcgg tccggcggag tttttcccgg gtggggtcac ggattcgatg tgcggggttg
    40321 gggatggaga agctctgagt tgacggccgg ggtcttcagc cggcatattg atgacgtagg
    40381 tgtcggaccc tgggaccacc cacccggcat gttaagatca caggggtttg cggcatgcag
    40441 ctgcagctgg tatttgttgg aaagaccgct ctttccccca tcgatgcggc cattgatcgc
    40501 tacctggagc gcctggttca cttcgttccc acccgggtgc atcacgtcaa ggcggagaag
    40561 atcctcccca aggcccgaga agcccgtatt cgggaaaagg aaagcgatcg cattctggac
    40621 ctggtgggaa aagaaggctc gctcatcgtc tgggaccgga gggggcgtgc gctggattcc
    40681 gttggccttg ccgggttcct gcaacagttg gtcgaccggg ggggtccaag gtttggatgg
    40741 tgacgggggg acccttgggg gtttctcccc ggctcctcga tgcggccgat tccgtgctgt
    40801 ccctctcccg gatgactctg ccgcacgatc ttgcccgcct ggtgttggtc gaacagctct
    40861 atcgcgccta caccattctc cggggagtcc cctaccacaa cgaataatgt gccggggcgg
    40921 gaggatttcc ccccgctccc attctttcgg caggacgccc ctcgagcggg gccggcggac
    40981 cagtcgacgg attcccgggg tcgagcgccg gtacttgagc ctcacccacg gggcgcccgg
    41041 cacctttcag acggcgacta cttcgctgac accgggaact tcctgcttga tgattctctc
    41101 gattccggcc ttgagggtca tctggctcat gggacaacca tggcaggcac cctgcaaccg
    41161 gaccttgacg acggtgccgt ccacgcccac cagttcaaca ttgcccccgt cacgctgcag
    41221 catgggtcga atcttttcca acgcctgttc cacttgttcg cgcattcccg tattcctcct
    41281 ttgaagtggt tttaggttga ttctataacc gtttgagggc tcaaaagcaa acaaaacgcc
    41341 ccggagaaga accgcacaag accccattgg ctcctggccg agggtgggtt tgcgaaccct
    41401 cctttcccct cccccggcct ctcccacgga cggaggagag aagaacgggc ggccgtcgag
    41461 gggcgggaaa aatgcgggcg gtcctcgatg gaggggagag aacttcccca gctccggcgg
    41521 cctctaacct ggaagacggt cccgatttcc ctcgaccacc gtaggaatgt tagtggaatt
    41581 aggcagttaa aaccgcttcc caattgattt atgcgctaaa cttcgataca ttggtcctag
    41641 ccctcgcccg gcggtgggga aggtgggaag accacgacga tccgaccgag gtcggcctgg
    41701 gggcaggatc tgcaggattt cggaccacgt ccggcagacc gggggaccat tccctaccgg
    41761 gaatcccgac gaatcgcgaa agcaccgact acaccggagt gacaaccatg cgccagtatc
    41821 gattggtagc gaggatctct tcccaggaac tggaacggag agtggacgaa ctggcccggc
    41881 agatcaaccg ggattaccag ggaaagcagg tactgctcat cggagtgctc aaaggggcct
    41941 atgtcttcct tgcggacctt gctcgacgac tttcctttcc cgtggaagtg gattttgttc
    42001 gtctatcgag ctacggaaac aaggccgagt cgagtgggtc cgtccggatc accaaggaca
    42061 tcgaagtgga catccagggt cgtcatgtcc tcgtggtgga agatatcgtg gatacgggaa
    42121 tcaccctggg ctggtttctg gaacggctcc gggagcggaa acccgaatct ctgaaactgt
    42181 gtgccctgat cgacaagttg gagcgaaggg aggtggaggt gcccgtcgat tacgtgggca
    42241 ttcgcctgga aagcggcttt ctggtgggct acggcctgga ctactcggag aggcatcgga
    42301 atctcccgga catccgggag gtggagttca ccgactgaag acaccgccgg ggaacgagct
    42361 tcgcggaccg ggattccggg gatcgccgtt tttcattaaa ggaagaggca ccatgatcgt
    42421 cacgtgtgaa agctgcaaga ccaaattccg attggacccg agcctcctca aagggcccag
    42481 ggccaaggtc cgctgttccc gttgcggaca cacctttcgg gtcgcccccc ccgaagagga
    42541 ggtgctcatt cacatcgacc tgtccgagga tgccctgggg caggaagagg cggccccgtc
    42601 ccgcatcgga acgcccgctc cgcctgctcc cgtccgcccc ggcaaacaag gccccttccg
    42661 aaagatctgg gtcctgatcc ttcctctggt tctcatcgtg gccggggccg tgtacttctt
    42721 cagtcgctcc gacagcaagc ccggctcgcc ttcgccccct tcctccgtgg aagtcaaaca
    42781 gccgaccgtg accatcctgg attccaccca ggcctatttt ctcgagaact cccacgccgg
    42841 acaaattttc gtggtggaag gcgaggtcgt caacgagtcc cccaaaccgg tgagctttgt
    42901 tctcctggaa ggcaagctct acaagcggga caataaggtg ggccaatccc agcgatgcta
    42961 tgcgggcaac gccatgaatc gaaaggagct cgcccagctg agtgtcaccg acattcagaa
    43021 tcacatgatg aaccgggagg ggaaggacct ctccaacgtc aacattcagc cgtcccggag
    43081 ggtccccttc atgctggtgt tccacaatct gccggagatg gatctgctgg gagactacag
    43141 cgtcgaggtg gtgagcgcca agctcgattg attttcggcc tgtggcgcgt ggttcggggg
    43201 cttatgggtc ggcaaggggg cttcggggga cgggcgggat gaagttaacc ccttgaagta
    43261 acaaaaaaat tgacaaggcg attggttcgg tgttaaagag cccgtgtcaa ctgcccgata
    43321 attgtacgga aacaatcgtc ttggatctgc agtaaggaag atggaaaacc atgctcgacc
    43381 tcaaatttgt ccgcgacaac atcgatcgcg tgaaccagat gctcgaggac cgtcacatgg
    43441 acacggactt gaaggccttc ctggtcctgg acgagaatcg gcgcaggatt cttcgccggg
    43501 tcgaagagct caaataccgg cgtaacgtgg cttccgagga gatttcccgc ctcaagaggg
    43561 aaaagaagga cgcatcccgg cagattgggg agatgcggga ggtgggagag cagatcaagg
    43621 cattggactc ggagctggcg gccattgaag aggcgttccg ggaggtcctc cttctcatcc
    43681 cgaacatgcc ccatgaatcc gtggccgtgg gcctggacga aaaggacaac ccggtcgtgc
    43741 gcacctgggg ggcggccccc gagttttcct ttgatcccaa gccccactgg gagatcggag
    43801 aaaacctggg gatcctcgat ttcgagaggg cttcccggat gacgggggcg cgctttgccc
    43861 tatactggga ttcgggtgcc gccctcgagc gggcgctcat ctccttcatg ctggaccttc
    43921 acacccggag gcacggctac acggaggtcc ttccgccttt tatggtcaac agcaacagtc
    43981 ttctggggac gggacagctt cccaagttca aggaagacct tttcaagatc gagggccggg
    44041 acttttacct ggttcccacc gcagaggtcc ccgtgaccaa cattcacgcc aatgaggtcc
    44101 tcgacgagga gaccctgccc cggtactaca ccgcctacac cccttgcttt cgctccgaag
    44161 cggggtctta cggcaaggat acccgggggc tgatccgaca gcaccagttc aacaaggtgg
    44221 aattggtgaa gttcgtttct ccggccacct cctatgacga gctcgagcgg ctgctggcca
    44281 atgccgagac cgtccttcag gagctgggca tccactaccg ggtggtgatg ctttgtaccg
    44341 gggacctggg gttttccgct tccaagacct acgacatcga agcctggctg cccggccaga
    44401 acacctatcg agagatttcc tcgtgcagca atttcgaggc gtttcaagca cgccgggcca
    44461 acatccggtt ccgttccaag ggccgttcca agaccgattt cgtgcacact ctgaacggtt
    44521 cgggcttggc cgtggggagg accctggtgg ccgtcctcga gaattaccag caggaagacg
    44581 ggacggtgcg gatcccggag gtgcttcgac cctatatggg gaacgcggag agaatcggtc
    44641 ccagaccgtg attctttcat gtcagaacaa caaggagcat ggaacatgac gaaaagtcag
    44701 ctcatcgagg cgctggcgaa ggcggaggga atcacgctga aggctgccga aacggcggtc
    44761 aacgtgactt ttcagagcat ggagcttgcc ctcattcgtt cggaccggct ggagattcga
    44821 gggtttggca gtttcaaggt caaatattac gaaggctaca agggaagaaa ccccaagacc
    44881 ggcgagctga tcgaggtgca gttcaagaag ctgcccttct tcaaggtggg caaagagctc
    44941 agggaacggg tcaacggggg ggaacccgaa gcggaaagtt ccaaggagaa atgagtcgcg
    45001 aggtctccgg gagacgaccg gagctccgtc catgtctctg tgccgcggac gggaaggaga
    45061 aggtcaaggt cccggcggtg gccgacgact atttctcccg catgaacaga taataaatgt
    45121 tgaccgcgat gatcaggacc gcaaccccca ggaattccat gagtttggag acgggaatgg
    45181 cttcccctgc ggcgggaagc ctggtctgaa cgaagacgag aaacaccacg acggaggcgc
    45241 tcaccaggag gattcggaag tgccttcgga gaatgagata accgagcacg gggaccagca
    45301 ccagccatgt gaaagggctc gagagcgcct tttgccagtc gacccgggcg atcatgtcca
    45361 taattttggt cacgtcgtat tccgacagaa accgaacgaa ttcctgccag gcggttttta
    45421 tctgatccac ggccgttatc ttccgtatcg aatgtcaaaa ctctccaaat cccctcgata
    45481 gacctcttcc tgcacatcca cccgatctac ccggccgaag ggactcccct cccggcacca
    45541 ctcgatcatg cgcgcaacct tgtcctcgtc accttcaaaa accgcttcca ccctgccgtc
    45601 ggccaggttt cgcacccacc cggcgatccc tccctcggcc agggccgcgt cccgcgtgta
    45661 ggcgcgaaaa aagaccccct gcaccctccc ggaaatggtt actcgaactc gcttcttttg
    45721 cacggacgag ccctcctcat ccatcggaat cgggtcttgc gccccctcga aggcgagcca
    45781 ggaactcctc ctcctccatg acctgcgtcc ccagggtgcg cgccttctcc agcttggagc
    45841 cggggtcctt gcctataatg aggatgtccg tggctccgct cacgctggaa gtaaccctgg
    45901 ctcctcgatc cgtcaacatc ccgatggctt cctgccgggt catcttttcc agggtccccg
    45961 acaggaccac agtcttgccg gtccaaaaat cctcgaggac gggagccggc ggcgaactct
    46021 cccggaaccg aattccggac cccaggaggt ccagcaccag ttcccggttg gccgggttgg
    46081 caaaataggt ccgaacgctg aaggcaacca cgtctcccac cccggggatg gcggtcaact
    46141 gggcctcgcc ggcatccatc acagagggaa tcgacgggag gtgcgccgcc aggagttggg
    46201 ccaggtgaga gcccacatgg gggatcccca aggcatacag aaaacggggc agggttgttt
    46261 ccttgctccg ttccacagcc tccaccaagt tgcttgcgga ctttttcccg aaaccgggga
    46321 aggattcgat gtcccggggt tgtagccggt agaggtcggc cacggagcgc aggacccctt
    46381 gctccaccaa gaacgagacg atcttcttcc cgagcccgtc gatattcata gcatcccggc
    46441 tcgcataatg ccagatggct cctttgatct gggccggaca attgcgattg aggcatcggt
    46501 ggacactttc gtccggaagc cgaaccaccg gctgcccgca cgacgggcac cacggaggca
    46561 ttcgaaaggc ctgttcgctt cccgtgcggc gggaacggac cacctcgaag acctccggaa
    46621 tcacgtcccc ggcccttcgc acccgcaccg tatcatgcag gaggatgcct ttccggtcga
    46681 tttcatcctg gttgtgcagg gttgccctct ggaccacaac tccgcccaca ttcaccggtt
    46741 caagaaccgc caccggggtg aggactcccg tacgccccac ctgaacgcga atgtccagga
    46801 tgcggctttc cgcctggtgg ggagaaaact tgtaggcaat ggcccaccgc gggctcctgg
    46861 atttttcccc caggcgccgc tgaagatcga gccgatcgac cttgacgacc attccatcgg
    46921 tttcgtaggg cagcctctcc cgttcttgct caagaattcg gaagaaatca acggcctctt
    46981 gaatgtttct acagaggcgg gagcgggggt tgacggggag tccccactgg cgaagccgtt
    47041 cgagtacttc ccactggctt tgaaaatcca ccccctccca ttgtccgatc ccgtaaaaga
    47101 aggctttgag cggtcggccc gcggtgacgg aggaatccag ttgccgcagg gacccggcgg
    47161 cggcgtttcg cggattggcg aaaaccggtt ccccggctgc ttcccggctc cggttcaggg
    47221 cttcgaagtc cttccggtcc atgtacacct cgccccggac agccaggagt gtggggacgg
    47281 gctgcccctc cggggaagaa aacagctgcc acgggattga ccggatcgtc ttcacattca
    47341 aggtaacatc ttccccgaca atcccgtccc cccgggtccc cgcccgtacc agcgtggaac
    47401 cttcgtacac gatttccacc gccaggccgt ccatcttggg ctccgccacg tacgccgggt
    47461 cctccgcagg acccaggagc tttttcaccc gtcggtcgaa ttcaaaaacc tcgctctcct
    47521 ccatggcgtt ttccaggctc aacatcggaa cggcgtgttc gaagggttga aaggattcca
    47581 ggggaggaaa cccgaccctt tgggacgggg agtccggcgt gacgagctca gggtgggccg
    47641 actcgatgcg gaggagctcc cggtagaggg cgtcgtagtc cgcatcgctg atctccggtc
    47701 ggtccagggc atagtacagg tagttgtggc gccgaatctc ccggcgcaag gactccaatt
    47761 cccggcggac cctgtcggct tcgttgttca tccggccacg tcctcacgtc acgaggttgt
    47821 tggttccgac ccggaggccg cctgccgggc aggtccttcg acgggtccat ccgtttgccg
    47881 atgatccggg tcccgcccct cagggaaggc ttcccaaagg cctgcgacgg cttccaaaac
    47941 ccattctacc agtttttccc gggcccggtg gaagcgagcc tcccgaaacc cgtgggtcca
    48001 tttgccgccg aagagctcat cggagaccac aagaaccgtc gccagctcga ttcccctgaa
    48061 atgggccacg ctgaacaagg ccgaggtctc catttccacg gccagcgccc cggcttctcc
    48121 gtaaagccga accttcgagg ccgtctcccg gtagggggcg tccgtggtcc agacctttcc
    48181 ttcatggaaa accagtcccg tttcacggga cttctccctg aggaccccca gaattcgagt
    48241 ggagggagcg gccttccccg ggtcctcgag gtaatggagg gacgtccctt cttcggagaa
    48301 agctccggta ggcagaacga cgtccccaat tcgaaccccc ggctgcaggg acccgcacca
    48361 tcccagggtc acgacccgtc ggactccgag ggcgatcatc ttttcgagga ccatggctgc
    48421 ctggggagcg ccgatcatgg gacccacaca ggccacctcc ccgtacgagc cgcccaaaat
    48481 acggacgtcg gccaggaaca aggtcctatc cctgcgggtt gcgcccgcat gaacccgaca
    48541 caaggattcc aggtcttggg aggtaaaaac tacaagggca aggggcggaa gcgggtcctc
    48601 tcgtcttccc ttccggggtt caatgagagc gggcctttca tgggtcggca ttcaaattgc
    48661 caggggaacc atgggaggat ctgttcgacg gggtttccgc tcccggacgc gacgcaggaa
    48721 agagcggaaa cccccgcagt ctattttacg gattctctca gcttgctgcc gggcttaaac
    48781 ttaaccacct tggaggaagg aatcgtgatt gtctggccgg tccgggggtt gcggccttct
    48841 cgctgggacc tcgtggcgac ctcgaaggtg ccgaacccga ccaaagtgat cttgtcctcg
    48901 gcggcaaggg ccttccctac agccgaaatg aaactgttga gcgccttctc ggctgtagcc
    48961 ttggtgatgc ccgctccctc ggcgatctta gccaccaact ccgtcttcgt cattttctcc
    49021 tcctagtcac taagggttgc aatgtgctcc tagcccctca cccattcgat atctggattt
    49081 gattgccctc gatcggcctc aacccgcatg gatcggcatt atacccggac aataccgcat
    49141 agcaagcaat aaatcggctg aaaagcagct ttggacaaca tttctcgggg gttttgcccc
    49201 tctgaggacc ccaaacaacc caacggaccc agcctcgttt cccctacccc acccctcttc
    49261 ccccgggggg gggagagggg gcttccaccc cctcgctccc ggcagccggc attgttaccc
    49321 tccccccccc gactcaccag caatcccacc tggttaggat tgaaaatttc gttttcgtag
    49381 aagcggcgtc ctgccgcgaa atgtggtcgc ggcaagatgc cgctcctaca gcaaagaaaa
    49441 gaggcgccgg aacgtgattt acaaagctaa acaggtatca ccgaccgacc aaccaacccc
    49501 tggccccccg actcccaacc aacctctccc cggtgggctc cgcttcgctt cacccaccct
    49561 acaccgttgc ccaacccctc gcccctcgcc catttcactt tgacctcatc gaccacatgt
    49621 gatacctgca acccaggagg gcctggagca tccatccccc gggaagccgt ccaaagggaa
    49681 agagggtctc ttgtttgccg aagtagccgt gttcagcgcc atggagaaga ctctccacta
    49741 tctggtcccc gaatccatgg agcacgagat gggtgcggga aagcgggtcc tggtccccct
    49801 ggggcgtcgg gaagccatgg ggctggttct cgccctggat tcctctcccc cctctcttcc
    49861 gaagaccgtt cgtcttcgac ccgtcctctc ggtcttggat tcccaaccgg tggtgcccga
    49921 cgagctcctt tccctgtgcc gttggatgtc cttctactac ttccatccct ggggagaggt
    49981 tttagagacc gtccttcccc gggaactctc cgtctctccc caagtctgtt tccggctgac
    50041 ccccggggca gcgacggcga aggcgtcccg gcaggaccgg gaaatccttc tcgccatcga
    50101 ggaggcgggg gccctctgca tgggggaact cgccgggagg atccccgaaa gcacgcatct
    50161 ttcccgccgg gttcgaagcc tcgaggggcg gggtctgctg acccgcttct tccgatggaa
    50221 ttcttcgagg gtttctccca agaaggttaa aacggtgcgg ctgatttccc cgccgtcccc
    50281 ggattttttg tctcggagcc caaacctggg gcgcctggtg ggacttctcg aggagtccga
    50341 aggaccgcac ccgctgcctc tcctgcggca ccgggtgccc aatgcggact actgggtcgg
    50401 gcgcctggcc cgggagggat gggtcgaggt cgataccttt caggaagtcc gagagtccgc
    50461 gtgcgcccag gacctggcgc ggccttcgga gatcatcccg acccgggagc agcaggaggt
    50521 ggtccaatcc gtcctccctt ccctcctccg aaggcgtttc gagacctttc tcctttacgg
    50581 gatcaccggg agcggcaaaa cggagatcta tcttcgactg gtggaaaagg cgatcgagca
    50641 ggggcgcggt tccatcgtcc tggtgcccga aatcgccctg agcactcaga tggagcccat
    50701 attccgccat cgattcgggg ccgacctggc ggtctggcac agtgggctcc cctcgggagt
    50761 ccgttacgat caatggcggg aggtgctcga gggaagaaaa aggattgtgc tgggggtccg
    50821 ttcggcggta ttcatgccgg tacccgacct ggggctgatt ctcgtggacg aagaacatga
    50881 tccgtcctac aagcaggacg accggttgcg ctaccacgct cgggacgtgg ccctcatgcg
    50941 ggccaaggcc ctgggaatcc cgatcgtcct gggatcggca accccctctc tccagtccct
    51001 ccaccagtgc ggcttgaacc ggtaccggct gctcaccctg tcccggagga tcatggaacg
    51061 tcccctgccg gaactggtcc tggtggatat gcggcgccag aagggccggg accggatcct
    51121 gtcggcggaa ctcaaggagg cgttgcggca gacgacggcg gacggaaagc aggccctggt
    51181 gtttctcaac cgccgggggt tcgcgacgtt tgtgctgtgc aacacctgcg gcgaggtgat
    51241 ccgatgctcc cgttgcagcg tgagcctcac cttccatcaa aagcaaaacc tcctgcgctg
    51301 tcattactgc ggcatggaga gatcgattcc cgagcagtgc cctttctgtt cccggtcttc
    51361 cctgtttccc ctgggattcg gtacggaacg ggtcgaggaa gagattcggc atttgtgtcc
    51421 ccgtgcccgc gtggtgcggg tggaccgcga tacggttcgc cgttccgggc agatggtgga
    51481 atgcttcaac gccgtgagga acgaagaagc ggacatcctg atcggaacgc agatggtggc
    51541 caaagggcat gactttcccg gcatcaccct ggtgggcatc gtgaatgcgg acatgggcct
    51601 ccagatgtcc gattttcgag cgggggaaac caccgtccag ctgctcatgc aggtggccgg
    51661 gagagcggga cggggggaca agccggggcg cgtcctgatt caaacctaca atcccaccca
    51721 ctacaccatc cggtcggtcc tggagatgga ctacctggga ttcgcccgcc gggagctcga
    51781 atcccgggaa agactccagt acccgcccca tgcccgcctg acgaggttcg tcctcacctc
    51841 cccccgggag gacgcggccg gcaaggcggc ccgggaactg ggccttctgt gcgaaaggat
    51901 tgccggggag cttcgtcccc ggggagtgaa catcgctctt ttgggcccct cccccgctcc
    51961 cctcacccgg ctccacaatc aataccgcta ccacctcttt gccaagagct ggaccagccg
    52021 ggagatgcag gccttcgggg aagcggtggt ccggcagatg aggcttcagc cctttttgaa
    52081 aaacgtctcc tgcaccatcg accgggacgt gatgaacagc ctctgatttt cggcctattt
    52141 cctgccgggt tctccgggat tcatcatggg gggagcggcc ttgtccccgg ccaggaggtc
    52201 tccccggtat tcgaccccgt agatgtagag catgaccatg gcgaaggcga ggctcagagg
    52261 cccgtacagg acaccggcca gcccgaagta ctgaacgcct ccgatgatgg ccaggaagat
    52321 gtaaaacggc gagagagaaa gagcgccctg gcccctcatg aggtagggac gaaggaagtt
    52381 gtcgaaggag cctaccagga gcacaaacca ggccgcaagg aagacggcgg acttcacact
    52441 gccgaagagc aacaggtaca gggcgcacgg cacccacacc agggcggtcc ccaccagggg
    52501 aatcagggac gagaaggcca ggaccgtgcc ccagaacaac ccggggattc ccaccaggga
    52561 aagaccgatc ccccccacaa tcccctggca cagcgccgtc aggaagcacc ccatgaacac
    52621 ggaccgggca accatgcgaa tggtgtccag gatccggtcc tcctgctcct ttcgcagggg
    52681 agaaaagtac cggccccgct cgatcatctc gaccccgtcc cgcaccaggt agaacaggac
    52741 gaagatcatc accaggaagt gcatgagcag ggtggcggcg ttgcccagga gggtcgcgcc
    52801 ccgggcaagg atgaattgtc cgacgttttt gctgacctgg agcaggttgc tctggaagtc
    52861 gattttgtgc agctcgaggg acccgaattt ctcctcgaac caatccaggt attcgaggat
    52921 cttgggatcg tgggtgagtt tcaggaggtt cccggccttg agccattcat tgatctggtt
    52981 gacggattgc agaccctggg ccaccagggc cgtcgagaag aagaacacgg gaagggcgat
    53041 gacgaaggtg aagaggaaca cgatggtcag ggcggccagg ttcttccttc ctcgatagaa
    53101 tcgaaccaaa tagacctgga gcggatagaa gagagaagcg aggacgatgg agaggatcag
    53161 ggtgtccaga aagggcttga ggatgagata ggagagatac agggaaaaga acaggaccac
    53221 caggagaaag ggcctgtagc tcttgccggc tccggggatc tcgctgcccg atccgttggg
    53281 tccgttcatg ccttcgagtc ctcagggaga tgaaatcttg gccgccttct gtcgatagag
    53341 gtccgttttc cgggaatccc ccatctcctt gtagagttcc gcctccagca ggtaggcctc
    53401 tttgagcttg gcgggttcct cctggaaaag gatttccgcc ttcttggcga actcgagggc
    53461 cttgctgcgg aagcccttca tccgccaggc ccgggccagg ttcagaaacg cttccccgtt
    53521 gtggacgtcc acctggatgg cctgctcgag gagcggaatg gccgaatccg gcttcttctg
    53581 cttgagggaa cccttggcct ggtccaccag gtgcatggac gccaggtgct ggggaggatg
    53641 aggctcctgc ttcttttccc tgggcggcgg gaccggttcc gagggggtcg aaaggtcttt
    53701 ctccttgatg gcgggaggag gtttcaggat ggcggtggtg gccgggggcg gtccggacaa
    53761 gccgctcgat ccgggaaagg gagtggtggt ttcgcctttg ggcggcgcag gggcaggctg
    53821 gggagcttgg gcccattccg gcgggatctg gacgaccttg ggagcgcagc ctgccggcaa
    53881 aaagcaaagc agcatccaca agacggcccc gcaatgccgc ttggttgcgg gaggcccggt
    53941 ggggcgactt tcgttgatca tatccgatcc ttctccttcc cccgggcgtg cagagcgcta
    54001 tggtctgctg tgcagccagc agaagtcctg gggaacgttt tccgatagaa agtactcgaa
    54061 ccttctcccg gggcaggaaa gcacggcgag tcgtccggac tgggggcaga tgagccgttg
    54121 aaccacgccg gggggcaccc gaaacttctg agggtgaatc catggtcgaa cctggctcat
    54181 gaaccgggcc caaagccgcc cggctccctt ggcccccgtg agctccgtgg gacgattgtc
    54241 gtcgtagccc acccagatga gtaccaggag gtccgtggta tagcccacaa accaggaatc
    54301 cctggtgtcg ttggtggtgc ccgttttgcc cgcacaaggg aaattgaccc ccaggcgtct
    54361 cacattcctg gccgttcccc gctcgacgac cccctggaga agatcggtga tgaggaacgc
    54421 cttggccgga gtggtcacgg tagccagatc gacgcttcga cgctccagga cattcccctt
    54481 gtcgtccacg acctctctga gagtcagtag gtacggtttc tgaccgtcgt tgtccaacgt
    54541 cgcgtaggcc tcggccaatt gcaggggagt cacctcgaag gccccgaggg ccagagacgg
    54601 cacgggctga aggggagtct gaatgcccag ggtacggatt gtctccataa tgttgtttaa
    54661 gccgactgtc aaggcaagac tcaccgttgc cgcattgagg gactgttcga gggcgtctcg
    54721 gaaaaggacc cgcccccggt agcgacgatc atagttcttg gggacccaga aagtcccgtc
    54781 cacgggatag gatgtggctt catccgccag caggtccgac ggggtgaagg catccagggc
    54841 cgccaggtac acgaagggct tgatggccga gcccggctgc cgatgggcgt agagggcccg
    54901 gttgtaggag ctctcccagt agtcccttcc tccccgcagg ctcaggacgg aaccggtctt
    54961 gggctgaatg gcgatgagca cggcctggag gggcgggaaa ggttcgcttc ccgggggggt
    55021 cgaggcggcc ttctcgagct ccttcaagcc ctcccggagg gtgttttccg ccgccagcgc
    55081 cagttcggga tgaagagtcg tgtagatgtt caatccttcg gattcgaggg cttccggggc
    55141 gtagagttcc tggagttgct gtcgcacggt gtccacgaaa tagggggcaa gattcacggg
    55201 aagggagggg tccgcaacgc ggacgggttc cagccgcgtt ttctcgtgat cgagaaacga
    55261 gatcttctcc agttcgagca ttcgcttcaa cacgaggttg cgccgcttct tggatgcctc
    55321 ggggcgcgac aggggagaat acatgttggg ggcgcggatc atccctgcca gggtggcaga
    55381 ctcggccagg gtgaggtcct cgacgtttct cccgaaatag gtccgggatg cttcccccac
    55441 tccatgaacg gcaacactgc cgcgctggcc catgtagatc tcgttcaggt acatctcgag
    55501 gatttcgtcc ttggtgtaca gattttccat gaccagcgac atggaggcct cgaggagctt
    55561 gcgcttgatg ctccgttccg gttcgagaaa gtagttcttg accagttgct gggtgatggt
    55621 cgagcccccc tgcaccaccc ggcgggcccg aaggtccgcc cacagggccc ggaacatccc
    55681 ccgccagtcc agcccgccgt gctcgtagaa ccggtggtcc tcgatggcca cgatcgcctc
    55741 ggtgagatgt cgggggacct ggccgatgtt caccagcagg cgactctccc gttccgggcc
    55801 gaaaaggcgc gccagttcca tgggttcgag ttcgagcacc gggatgtccc ccccggcgga
    55861 aacgatccgg gaaatcttgt tttgctgaaa atcgaaccgg accgtctggg cgggaagagt
    55921 gcgcccaggg aactgaaacc cgcgaatgtg aaccacgagg ctgttgggaa agacccggaa
    55981 ctctccgggg ttcaatgtgt cccggatggc cgtctggtag cggcgctcct caaagagccg
    56041 ccgcacatcc gaaagggaca gggactgccc cgggtacacg ggcaccacgg cggaaaaaac
    56101 cctcgatgga acgctccaac ggcgcgacga aaacctcttc tggatctcga cggtcaggtg
    56161 gtagaaaaac gcgacggcga ccaggagcgc cagggagaaa agcagaatca gcaccttggc
    56221 aaccagccag gtcacggacg gctctttggg tcgaattctc ataaggaaag atcgaaagat
    56281 ctccgggacc gcaggtgcct agggttcgtc atcttctctc caactggacg acgcattcga
    56341 tatgaggcgt atggggaaag agatcgaaag gctggacctt ccggatctca tattgttccg
    56401 ccaggaggca gaggtctctt gccagggtcg ccgggttgca ggaaacggcg acaatcctct
    56461 cgggtgcaag ctcgaggagg gtcttcaaca ccttggggtg catcccggac cggggaggat
    56521 cggtgatgac gacgtcggga acttcatgag gggaaaggga ctcgacgtcc ttgcccaggg
    56581 tgtctttcag atcgcccgcc ttgaacgtgc agttctcgat tccgttcctt cgaccgttgt
    56641 catacgcatc cgagatcgcc tcctcgatca cctcatatcc tactacccgt cgcactcgag
    56701 gtgcaacaaa aagggagatg ctgccggttc cgcagtacaa atcccagacg gtttcttttc
    56761 ccgtgagttc ggcaaagcgt aaaacggttt cgtagagtcc ttccgcggcc agggggttcg
    56821 tttgaaagaa ggagtgagcc gagatggaat accggagatt ccccagcctc tcggtgatgg
    56881 ccccgggccc gaaaaggact cgactcgaat ccccaaaggc cacctgggcc ttcttggagc
    56941 tcacggaatg gacgaaggag gtcagccatg gaaaccggga agcgatttct ctgcccagtt
    57001 gatccacccg gggagcgtat tcgggaattc ctgcggtgat gagatgggcc aggctttccc
    57061 cggtgcgctt tccttcccgc acgaccagga agcgccagta cccctggtgg ttccgggtgc
    57121 tgtaggccgc gatcccgctt tccgcacacc actttcggac aaagcggacg agctccaccg
    57181 aagcttccga ctggagaaag caggactcga cgttgaatac cttgtcgtaa aacccccgaa
    57241 tatgtagccc cagggcgagg gacttgtcgt agacggtgtc tttggcggcg atctcgaggg
    57301 gagaaagcca ccttcgagga gaaaaggtgt actccatctt gtttcggtag aagcgctgat
    57361 ccggggaggc aatcacggga gccgtccggg tgtcctcgat gcccgagatc ctctcgaggc
    57421 aatccgccac gtgggaccgc ttccagagga gctgggtctc gtagtcgatg tcctgccacc
    57481 ggcacccgcc gcaaacggaa aagtgcggac agaaagggtc ccggtagtgg ggagactggc
    57541 ggaggacctc caccggggag gcttccacgt attggggctt cttccgggtg attcttgccc
    57601 gcaccagctg acccgggatg ccgtggtcga ggaagaccac cagcccgttc aaatatccca
    57661 gggcgctgcc cccgaaggcg agcttttcca cctggacctc gatttccgat cccttccgta
    57721 tttccgtcat gttctatggc cgatccgcga ttctccgggg tccttccggc cttcgccggt
    57781 aggacggtaa acgatcgtta gcggcccaaa gggtgaccct cgaggatctt cgtttcccta
    57841 acggatcaca ctcccgtccg tatgccgacc tctttggcaa tggcggcgag gtgcgatgcg
    57901 tacaaacctt ggggcgattg ggccgtcacg ggggtcaggg tccgggcgct gtgctggacc
    57961 tgcgcctcca aaggcaggca gcccacgaaa tccacggtgg tggagagcat ctttcgagcc
    58021 acttcctgga ttctccccgc cacatccatc atgatggatt ggtcttcgac caggttcagc
    58081 accagcttgg gacgatagga ttggaggacc tgctggataa gaaacaggtt ttggggctgc
    58141 tcttttcgag ccgcctccac cagttcgctc atggggattg cccgtcgtcc gtcggcaggg
    58201 ctggtgactc gggcaatgag ccgggccaaa cgctcgtcct tttttaggcc caggtccgat
    58261 tcctcgccga aaatccggtt cagcttccga tacagggcta ccttgatgaa gttgtaggcc
    58321 tcgagatagg cggccgggtc gcaggtggcc ataacgatcc cgatatccgc cgaaaggaag
    58381 aagtcgatga tgttgaacga ggtgtctccc ccgaggtcca ggatgacgta atcggcatcg
    58441 attcgcttga tggacttgat gagcttgatc ttcctcgaga agccgatgtt ggcggccccg
    58501 aaccgggagc tgtcacctcc gatgagcccc ggaccgtatt gggtctcggt cagggcatcc
    58561 tcaatccgcg gggtgcgctt ttccaggaag tcgttgatgc accggggcag gaaggtcttg
    58621 cccaggtaaa ggtgaaggtt ggctcccccg aggtccaggt ccaccaggac gacccgatat
    58681 ccccggctcg agaggagcac cccgaggttg gccgcaaaga ggctcttgcc gatgcctccc
    58741 ttggcccccc cgacggcgat caacctcttc cgggcttcgg ccgaggccgg ttccccttct
    58801 cgccccgggt ccgtccgcag gtagctgcgc agcagatcgt aggaatccat gaccttgagg
    58861 agacgctctt tccgcctctc gatcatttcc cggggctcat cgcggtgcag gtccgggtga
    58921 tagcgtttgg ccttctcccg gaaagcccgg gtaacgcgct cgaggtcgag acgggccaca
    58981 aaggcatcgt cccgagcctg ctcgacggaa aacaggaaag cgcatgccac ccgaatccgg
    59041 tagatttctt cccctttgag ctcatggagc ggcaacacgg gacttacgtc tcccggggaa
    59101 cctggggccg gcgtttcgaa aatacgggca cgggtacgtc tccgaagtca taaaacgctg
    59161 cattaaggct ccactttcgg tggaaccttt ctccagttct ggatcttgtc gatgatctcg
    59221 ttcacggaaa gatcctcctt gagctcgtag aacccatcgg gctcctccgg ctcccgggac
    59281 ggcctgcggg ccgaggccgg catctccccg ggacgaccta cggcggcgtt gtagatctgt
    59341 ttgcggggga ccttgggaat tctcacatgt ctcacgtcca catgggccgc ggaaatcagg
    59401 ccccataccc gcatcttctt ggaagaaccg gcaaagtcct ggacggcggc attgagaaac
    59461 tcggcgagct cggagctgtc cccgggcgcc gtggtcggca gcggcacccg ccgataaccc
    59521 aggagattcc accgatgatc ccctgtctgg gctatgagga ccattcgcag ctgctggtgg
    59581 cccaggtcga ttcccagggt ggtccgggtg ggaaacggca ggagtccgcc ccgggcggaa
    59641 agtcggctcc acggcgaggg cggggaggtc tcggacaagg ggctcggtgg agaggcgtcc
    59701 ttggaagagg attctccccg gatggcctgg agaagcctct ccgtggcgga aatctccttg
    59761 tcggaggaaa acacatcctt cattcctacg ttgtcctcgt ggctttggcg tctagaagga
    59821 cggtccggca aagcggagct catgcgccgg gcacccaacc cccttgtctc ggggcagacg
    59881 gcagagaacg acatcccgcc ttctgaggct gggctaagca atactcatgc ccaggacgaa
    59941 tcccgcattg ggattgcggt catgttgtct tgggaggcat ggcgagggac aattttgtga
    60001 gaatttgctc aagaatgcaa gataaaaaga attactggac gggtgagccc gacggcgatg
    60061 cgacaaaata gggcgggcga tcgcaggccg aactccaagc tccggttttc atttgacccg
    60121 gcggaatcgt cctgggtatg tttagcggac ttggcggcgg aggaagacga tctcggccct
    60181 cggccctccg gtgaccgcca ggacccctct tccgtcgcag gccggttccc ggcacccacg
    60241 gaggattcgg gcgcccgggg tccggctacc gtggggtcgg aggtcggccg ggacgagggt
    60301 ccccccgggg ctccaaagtg ggagcccgct tctgcggagt cggccacgag gtacccggaa
    60361 accggtccac ccaatacggg agggaaacga tcccccatga gtccctggat ccaaaagctt
    60421 cggacctacg gccgtctggt caagttcagc cataccgtgt tcgcccttcc ctttgccctg
    60481 tccgccgtgt tgctggccca tcgcgagcat cccctcaccg ggagcgtcgt tctctgggtc
    60541 ctcctcgcca tggcgggggc ccgttccgcg gccatgggct tcaaccgcct ggtggactac
    60601 gagtacgatc gcctgaatcc ccggacgtcg gaccgtcctc tcacctcggg tcaaatcgac
    60661 cggtcctcgg tgaagatgtt catcgcaggc tcctccctcc tgttcattct cagcgccacg
    60721 gccctgggaa aactctgctt tctcctggcc ttcccgaccc tggcgatcct ctttgcctat
    60781 tcctatacca aacggttcac cccgtactcc catttcttcc tggggctggc catcgggctg
    60841 gcacccctgg gggcctgggt ggccgtctcc ggggcactgg accctcgaat cctcctcctg
    60901 agcctggccc tcttgaccta cattgccggg ttcgacctgc tctatgcctg ccaggatatc
    60961 gacttcgacc gcacgaaccg gctgttcagt tatcccgctt cctacggcgt ccgaagaacc
    61021 ctccggttgt cctcggccct gcattgcata acgtttgttc tcctgaccgc cctgtatttt
    61081 gccttcgacc tgggaatccc gtacctgggc ttcctggtcc tcatcgggtt tctcctggcc
    61141 ctggagcacc ggctggtccg tccggaccga ctggatcgga tcaacctcgc ctttttccat
    61201 gtaaacagcc tggtatccat cctgttgttt ctggcggtgt ggacggattt gtggctaggg
    61261 gccaggggct aggggcgagg ggtatagggt gggcagcgcc aagcgccgcc caccaaacag
    61321 cgcatccgtt tgaagggaag ccgaaactca gccacgggag tcctgccctt atgaccacgg
    61381 cataccgaaa tctgcgggag tttgtggagg ccctggatcg ggccggggaa ctgctgcgca
    61441 tcccggcccc ggtgtcccgg cacctcgaga tcacccacat caccgacctg gcatccaagg
    61501 cccccgccgg gggcaaggcc ctgctatttg aacgggtgga ggggttcgat tttcccgttt
    61561 tgaccaacgc cttcgggagc gagcgccgaa tctgcatggc cctgggggtc tcggacctgg
    61621 aatccctggc cgggaggctg cggcgtttca tcgaaatcaa gccgcccaag tctctcaaag
    61681 acgggctgaa gctccttccc ttggggctgg acgtctttcg gtttctccca cgcaaggttc
    61741 gccgggcgcc gtgccaggaa gtagtctacc gaggccggga cgcggacctg ggcaaaatcc
    61801 ccgtcctcca gtgctggccc caggacggcg gtccctttgt gacccttccc gtggtgatca
    61861 cccgcagcct cgagacggga aggcgaaacg cgggcatgta tcgcctgcag gtctacgatg
    61921 cccgaaccac gggcatgcac tggcacatcc acaaggacgg gtctcactac taccaggaat
    61981 accggagggc gggaaagagg atgcccgtgg ccgtggccgt cgggaccgac ccggctacca
    62041 cgtacgcggc cacggccccc atgcctcggg gcatcgacga gatgctcctg gccgggttca
    62101 tccgtcgaaa gcccgtcccc atggtccagt gcatcacggt ggacctgcag gtccccgctg
    62161 aagccgagtt cgtcctcgag ggcacggtgg atcccaagga actccggatc gagggccctt
    62221 tcggggacca cacgggatac tactccctgg tgggggacta tcccgttttc cacgtcaccg
    62281 ccatcaccca tcgtcgcaac cccatctatg ccgcaacgat cgtggggcga cctcccatgg
    62341 aggattgcta cctggccaaa gccacggaac ggatctttct gcctcttctc aacacggtgc
    62401 ttccggaaat ccgggacttc tggatgccgt gggaaggggt ctttcacaac atcacggtgc
    62461 tttccgtgga aaaggagtac ccgggccacg cccgcaaagt catgaacgcc gtctggggcc
    62521 agggacagat gagcttcagc aaggtgctcg tcctggtgga ctcggacgtg gacctgcacc
    62581 gtccccggga ggtcctcgag acggtcctcg ataccgtgga cctcgcctcg gacctctacg
    62641 tcaccgaagg ggtcctggac gtcctggacc acagtgcccc cgagcccctt ttcggaggaa
    62701 agctgggcat cgacgcaact cgacgcatcg agggagaaag gccacgggtt cgccccacgg
    62761 gagtcgtctc tcctgtggaa ccctcgagga tcgaaaaggc cctgagagaa gtctccccaa
    62821 acctgcgatc cttttgcgtc cctgagctga gggcccgaaa tccgttgctt ctgctgaacc
    62881 tggaaaagga cgggaaaacc acgggaaagg ccgttggcca aaggatcctc gaacacaagg
    62941 gactcgagag cttcgctctt ttcgtcctct tggacgcagc cgtggacctt cgggattcct
    63001 ccctcactct ctggaagctc ttcaacaacg tggacccgct tcgggacctg gtccgccggg
    63061 atcgccgcgt ggttctcgac gccacccgaa aggggcccga agacggtcac catcggccct
    63121 ggcccgacga cattgttatg gatcccaagg tggcggcaag agtccgggag agagccaggg
    63181 agctcaacat tgaaccgttt ctgaaaaagg agtaacattc cggaacggaa cgatttgcgg
    63241 ccggggaacc ggaccttttt gccgaagcaa ggaccggggt tcggcgtgcc cggcgccccg
    63301 aatcggtccg tctccataga acgccccacg gacaaggaga cccatccgga tcgtgaatcc
    63361 aatccctgac ccacacgaat gcctgcgtcg catggatgag acgcggatgc cggagcatat
    63421 tcgaagacac agccaccgag tggcggccgt cgccctgtac ctgggcagac ttctcaatga
    63481 gaacggatgt cgcctggacc ttcgcctggt ggaggccggg gcgctcttgc acgacgtcgg
    63541 gaaatcccag gccctcgcca ccggggaaag gcacgagatg ttgggggccg aaatcgtggg
    63601 gcgatggggc taccgtcccc tggtgcccat cgtgcgggac cacgtacacc tggacccggc
    63661 aacggtccgg ggccccatta ccgagtccgt cctggtgaat tacgccgaca agagggtcaa
    63721 gcacgacgag atcgtgtccc tggcggaacg cttccgggac ctggtggaac ggtacggaag
    63781 gacgcccaag cacatcgagt ggctcgaaga aaagtaccgc ctcttcgacc ggttggagaa
    63841 gaaaatcttt ggacatctgg ggattcatcc tcaagaggtc ggcctcatga gccttggaac
    63901 gaatccctag gagacaggaa ccggagcgat ctatgggaaa aaaacttacc gtggcacttt
    63961 tggccggggg aaaatccgcg gagcgggaag tatctctgaa gagcggcgag caggtgttcc
    64021 gggctttgga caaaagccgc tatgaggtcc tgcgctacga tccccgagac gacctcccac
    64081 gcctggcggc ggatgccggg aaaatcgacg tcgccctcat catcctccac gggcggctgg
    64141 gagaggacgg aacggtccag ggctttctgg acctgttggg aatcccgtac cagggcagcg
    64201 gggtcctggg cagcgccatc gccatgaaca agatcctcag caagcagctc tacatccagg
    64261 ccgggctgaa ggtggctccc tacggaatcc tggactcccg acagagggaa gccgggatgg
    64321 aaacggtccg aaacctgggg tttcccgtgg tggtgaagcc ggaacacgaa gggtccagta
    64381 tcggtttgag catcgtgaat cgggaagaag acctggccgg ggccctcgag gcggcctggg
    64441 agttcgatcg ccgctgcctg gtggaaaaat acgtccgggg aatcgagatc acggcagggg
    64501 tgttgggaaa cgaccatctc caggcgttgc ccttgattga gatcgttccc ggggaaggac
    64561 acgccttttt cgattaccag gccaagtaca cccccggggc atcgagcgag atctgcccgg
    64621 caaggatcag cccggaactg accgccctgg cccaagacca tgcctgccgg gcccaccggg
    64681 cactccactg caagggctac agccgcacgg acatgatcct ttccccggag gaaaacgaag
    64741 tctacgttct cgagaccaac accatcccgg gcatgaccca aaccagcctg ctgccccaag
    64801 ccgccgccgc cgcggggatt tcgttttcaa gcctgctgga ccgcctcatt acactggcca
    64861 tggaacctta ggtggccgcc gacccggcct tgtcgaaacg gccgcgggga gctcccacgt
    64921 tgtcgaaggc cgagttgaag tcccggtaga gggtcttgta gtccgcaatt cgatgccact
    64981 tcttcacgta gccttcgggc agggagtcca tccaatcgat gccctgccac tgggtggggt
    65041 tgggaaaggc gtaatagggg tactcccggg ggacgtttcg gatcaggatg acctggggat
    65101 cgatccattc gagataagcc agataaaggc ggtgcatgcc gtcgttgatg atgttgaccg
    65161 tcttcccgtt ggcctccacc cactgttcga ccacgggggg cagaaggtcc acgggttcgg
    65221 tccctccctc gatccacacc ttcaggtagc cgtccaggcg gaagagatcg tactggaggc
    65281 gggcaagctc ccatttgagc tgccggacct tcatgagtcc ctggaccaat atgtagcgct
    65341 gggccgggtg gagtcgatcc acccccaggg tttccaaccg gatatccgct ttctcgtaga
    65401 ccttcacgtc tcgccttcga agcatcgtta cctgccgcag attgcgcacc aggtcttcct
    65461 cgggtcggtg ttccacgcgc tcaattcgaa agttcatggt ttcctcgcag tctgggaggg
    65521 ggctttggga acggccctga aagaaaagga gacttcggga tttttcaccc acggccccgt
    65581 cagatggata tgattttctt tcggatggcc atcttggtca ggtcgttgac gctctggacc
    65641 ttgagcttct gcaggatgtg gtatttgtgg gattccaccg ttttgggact caggttcagg
    65701 cgccgggcaa tgtccttaat ggaggcccct tccgcaagga gctggaacac ctcccgttcc
    65761 cgcaggctca gcccttcgaa ggggtccttg gccatggcct gccccggccc tgcgttcgtc
    65821 aggtggtcca gcatgatctg ctgcaccatc tcgctgaaat acttccttcc cgccttggcc
    65881 tccttcaacg ccgcaaggag ctgggacatg gggtcctctt tgaggatgta ggccgaaatc
    65941 cccgcccgga acaggtcgat gacgtattcc ttgctcgagt acatggaaaa gatgatgacg
    66001 gcaacgtcgg gattgatctt cttgatctgc agggtcgagt cgatcccgtt gagatcgggc
    66061 atggagatgt ccatgatcac gatatcgggc tcgagggtcc tcaccttccg gaccgcgtcc
    66121 cgaccgttca cggcctctcc gaccacttcg aaatcctcgt gatctcgaag ggcgctcttg
    66181 atcccttcga tgaccacgcg gtgatcatcg gtgatgagta ttttcgcctt tgccatgtgg
    66241 atatcccttg ccccggtgag catccgggtc gtcgacttcc gatgaatcgc ccggaaaccg
    66301 attcgaccca caaaaagggt ccgtctcagc gattgggagc cacgggattt atacaccaaa
    66361 caccgcgggc gctcaagcgc tccccatccc ataaggattc tcccccggca ctcgatcttt
    66421 ccaagttcgg acttgaatcc aatggcaagc atccctataa taccctcaat tgttgcgcgg
    66481 atccaccggg tatgcacgtg agaatcatca atcaatccaa cgggggtgcg agatggcaga
    66541 aaagagggaa gtctgtacct gtaccaagtg cgggaatgaa gcggaaatga tcgttacgtg
    66601 cgagctcgtc gaaatcgaga cctcgccggg caccaagcaa aagaaacaga aggaaaaccg
    66661 gaaatgcacg tattgcggca atgaagccga catgatcatt gatttcgacg aataattcct
    66721 cgaaccaaca gcgcttcttg cccagtgcgc ccgcgggttt ctgcgggcgc ccgcccacat
    66781 cccccgaccc ccccgatcat ccggacccgg tcgttcgaac ccttcattcc ggcgggaggg
    66841 cgctttcttc ttcctttccc ggtgtttccc tttagaacag agccgggtcc cgagtttgcc
    66901 ccggagaacc agcgcagctc cggtgggaaa caccggaggc acttcgggca gacaccgttc
    66961 ggcggatatg ccccgggacc ccccacatga agatcgatgg cacgagcagg aggaagccac
    67021 gcgtgtatcc gagtcactga ggagtccggt gctcgccgtc ttatgtgcgg ggatttcgtg
    67081 gttgggttga cttctgcggg gttcgtcttc tccgccttcg acgaacacag ggacctgaaa
    67141 acctatgaag ggagcgctgc ctgcaccccg tgccacgtgg aagaggcccg ggaggtccat
    67201 gcttccgccc atcaccagtg gaagggcaaa accccgcagc tccccgacct ggggttcgcg
    67261 ggaaaactcg gcggcattaa tgacttctgc acctatccca acctcaattt cctcttcctg
    67321 atgaccaact tgggcggagc gaccgtggga gccggttgcg ccgcgtgtca tcccggcatg
    67381 ggagccaaac cccaacgggg tgccacggcc gaacaaccgg ccaatgtcga ttgtctcctg
    67441 tgtcattcgg acgcatacaa acgggttgcc gtgaagcaga ccgccggaat gcgctttgtt
    67501 ccggaccccc cgtagacatg gggtccgtgc cggccaacat ccacaagccc accaacagca
    67561 cctgtctgaa tcggtgtcac gtgagagcgg ggggaagccc cgggggcaag cagcgggaca
    67621 ttgacctcac catggagaat cccccgcgga ccctggacgt gcatctttcc gcctccggag
    67681 ccaatctttc ctgcctcagc tgccacaagg cgaagaatca taggattgcc ggacgaggaa
    67741 acgacgtccg tgaaacggac ctcgccaaaa agatcgcctg cgccgattgt cacggcgccg
    67801 ccccccaccg cgattacgac ctggacgctc acaccgggag agtggactgc accgtctacc
    67861 acattcctcg ttacgcacgg ggacccaacc cgacggagac ccaccgggat gcccgccggg
    67921 caaagtcgga tccggccacg ttgcggtacg agccccctcg agtcttcgcc tcgaacctcg
    67981 tcccggaata ccttttccgg aacggcgatt ctcatttcta caagttcggc gaccccgttc
    68041 aactgcaacc caacggacgc ctgctcctta ccggtcccct gggagacatc cgggaaccca
    68101 gcgccaaaag atctttgctt tcaaacccca cacggcccgg ttggggtacc atccggtcac
    68161 ctggcgcctc ctgccggtaa aatccaaggt tctgtgggag acgggaaagc cgattgaggc
    68221 attcgttgcg ggggccaagg acctgggctg gaagcttccc caaggggtcg ggttcgtttc
    68281 ctcggatcgc tacctgtccc tgcaccacca ggtggcgccg aaaagatatg ccctggaatg
    68341 cgcggaattc cgcggcggaa cccgcatgaa cttcgaacgc ctgggatacg cacccaagac
    68401 cacccgggac ggcacccccc tgtgcatcag ctgtcacgac cgggaagacg acaagcccga
    68461 ttactatgag atccacgcca tccacccccg ggaggaagga gtcaactgca agacctgcca
    68521 caaattctcg gggacccggg agggatggga agactgattc ggcttcgaga gagcccggac
    68581 ctataaacag ctttggcgga cagccttccc aaacgccgaa aggaaggtgc ccgccaagcc
    68641 gggtcattcg atagaagggg acggagtggc gttaggaaat gtagtcttcc gccacgtagc
    68701 gcccttcaag caaaatcctc agggcttcca ggtcctcttc ttcttccacg cccgcaaatc
    68761 gttcccactc gggaagatgc cggtctcgat gcacgtcccc gcctcctccc aggaagtccg
    68821 cgtaggattc cacgtccagg tgggtcatcc aaaggggcct tctttccttt cgcagcgctt
    68881 cgatattcag ttccatggca cactccatgt tgtatccata tccgtaggtt gcgatatatt
    68941 cactaagaat tctccaatac gtcggtatgg taaccatgaa agaaaggatg tcaatgaccc
    69001 aagcccgggt tgaacgactc cggaagcatc agggcgtcat gtccgcgacg gcgggcatcc
    69061 gacggggatg caccctcttc gcgaattccc gcctgcgcgg gaacgagaaa gggggactca
    69121 taacggccaa atcgctcact ttcggacgat gcttgttgga agggcggggt cgggacggtg
    69181 ggtgcggctg ggaaccgtta gcctctttca agatgtgaat ctctcgcggc cctcgatccg
    69241 gcaggggaac ctccccgtag aaacgcatcc ccagcctttt caagactgct ttggacggct
    69301 cgttcatggg atggaccagg gcaagcatgc gggaacatcc cagatgccgg aagccgtaat
    69361 ggatccgggc tcctccgatc tccacggcga accccctgcc tcgggcgctt ttcaccagtg
    69421 cggacccgaa ttcgtagtcg tcctcggaaa gatggccgca cggaacgatt cctccgaaac
    69481 cgatcctctc tcccgtctcc ttcaagcaca agggagccgg ccccgcgggc tccgccccga
    69541 atggaaaatg ccgcccgatg aactcgacgg cttcctcccg gcccatggga acgcccccga
    69601 agaggtactt catcgtctcc ctcgaggcaa acatcccgca cagataatcc cgatccgtcg
    69661 gttccatccc gcgcaggatc agtctcgggg tctcgatcac gcggcccacc gttcgcctcc
    69721 gtagggccac gcccttattg gccgccgcac agttcccggg cggttgtctc cgccagttcc
    69781 atgacttctt cacaccgctc gacctccccc ggcgccgcga ccccgaaggc ccgtatcaac
    69841 cgcgtgtcgg agaagccgta tgcgcggaag aagtaatcga acttcttgaa gatgtccgta
    69901 aaactgtttc ggtcgggatt gttctgggca aggatgaaaa ccagcttctt cccgggttcg
    69961 aggcgggatt tcctgggatt gacgatgaag tcgggcacca ggaacgagta cgttctgtcc
    70021 aggaaggcct tcatttggga ggaaacgtcc cagtaataga cgggggatgc caggacaagg
    70081 aggtccgttg cccgcacggc atccaagacc tcggtgaggt cgtcttcgag ggcacagcgg
    70141 tcgagccggg tcttgcacgt catgcacccc tggcagccgc gaaagctcag gtcgttcagg
    70201 gcaaaagtcc gcacctccgc ccccagtcct tgtgccgtgg aacaaaaccg ctccgcgatc
    70261 atggaactgt ttcccttcct cctcgggctt ccgagcaggc agacaatctt catcttcatt
    70321 cctccgaaag acaggcttcc ccgggccggg atgtatcggt ccgtgccctg ccggggtcac
    70381 caaagcaaaa agggctgcag tcgtgtgccg caacccttct tttttcctgg tggcgaggcc
    70441 cagaatcgaa ctggggacac gaggattttc agtccactgc tctaccgact gagctacctc
    70501 gccacgaacg ccgcggatgt tactgattcc acggacgggt gtcaagcaag aagtgtcggc
    70561 agcactgggg tgccgacgga tcgtccggga ttttcccgag gggtcgagga tcacgagggc
    70621 ttcacgggcg gcttcttctc tcccttggga gaggtctcct ccaactcttc gagcaggcca
    70681 tcgaggtcgt cgtcggtaag ctcgagaacc aggtccgaat ccgagatttc gaggggttcc
    70741 gtggggagtt ccgtcggggc ctcccggggc cggtcgggta cgatgggctc gagcccgatg
    70801 attccgtcgg cggtggtctc gagctccatg gtctgcctga gctcctcctc gtcgatcacc
    70861 agctcgaact cggaggtggg aggcttcccc tggacgaagg cttcgtccag gtccagggtg
    70921 atctccggga cttcgagggc cggctcgggg gccggaggag cagacaccgg tcggggcgac
    70981 gggtactcga ggggctccaa ccggagggtg ggttcttctt cgagcttcag atcgagttcc
    71041 gggaccgggc ccgggatcgt cccaaccatg ggccctgccg gggtcttttc ctgcgcgtca
    71101 aaatcgagat ccagggaaaa gtccggctcg gggactttct ccgagggatg ccgggcactc
    71161 ttgacgattc gatccatttc ctcggggctg atctgcaggg tgggagcgtc ctcgaggtcc
    71221 aaagacaaac ccggggttcc ggcagtctct ttgctttccg gagccgctgc cggggatgcg
    71281 gcgggcgcct gggttgcggg cagaggcacc tcctcgagga cgtccgcatc cagttccagc
    71341 t
//
LOCUS       DAOOVC010000002        70486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_210_length_70486_cov_7.040522, whole genome shotgun sequence.
ACCESSION   DAOOVC010000002 DAOOVC010000000
VERSION     DAOOVC010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 70486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70486
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210_length_70486_cov_7.040522"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /locus_tag="PLM79_00345"
     CDS             complement(<1..1340)
                     /locus_tag="PLM79_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV84781.1"
                     /translation="MTGRIKILVVDDELIVRESLVGWLKKSGHEVSGAEGGRRALEML
                     GEREFDLIFLDIKMPDMDGLEVLHEIKANYPESMVVMITAYGSVQTAVEAMKSGANDY
                     LMKPFEPENLALLVEKLLQQKKILDENLLLREHVATRVRCEDLVGVSPCMQQLFDLIE
                     EVAAVDSPVVLRGETGTGKELVARAIHSRSGRSYSPFIPINCGAFPETLLESELFGHE
                     SGAFTGAVRARKGRLEMAHGGTLFLDEIGEIPLKMQVDLLRVLEEKTFHRVGGTKEIS
                     VDFRLICATHRDLPKEIERGVFRRDFYYRLNVIEIEVPPLRLRKEDIPVLAQHFLERS
                     RRETNKPIMGIQSPAMELLARYEWPGNVRELQNAVERAVVLAKGRHLTRDDFAFLLRT
                     GGRTVPQSLKENEKQHIDHILKQCGWNISKAAGVLEVSRITLHNKIKKYGLTPPV"
     gene            complement(1380..2903)
                     /locus_tag="PLM79_00350"
     CDS             complement(1380..2903)
                     /locus_tag="PLM79_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84782.1"
                     /translation="MKANKVMNIAVIGGGKRCAAFLEMLDAMRFPHLEARIVAVADPN
                     EDAVGMRIARERGTFTTRDYNDFFKIKDLDLVIELTGNETLLLDFLRHKPKNTRVLEA
                     TISRLFSDMIRFQEQYLLEKRQLELIQGIVDSIFSSIQDRVLILQPDFKILDANEAVL
                     QSLGATRQDIVGKRCYEISHWTVSRCDEKGHHCPLKESLETGRTAHAIHEHFDRNNET
                     RTCEVTIVPLKNKQGKIELFLEIMRDITDELVKRVEQKTRILTMNLARLIHEDKMIAL
                     GKLVASAVHEINNPLTGIHALARLVSQGLRAGPPSPEDLKQYQYYLDLIDTESARCST
                     IVGNLLSFARQKKMEQGYFQLNDVVRRVAALFSHRMQLQGARLELALTEGLPRMYGDS
                     GQIQQCLINLLFNALEAMPEGGTVTVRTCWEASSNLIRLEIEDTGAGIPQGMISHIFE
                     PFFSTKSQDKGVGLGLSVVYGIIKEHQGSIYVKSEEGKGSTFIIRFFALEDPEGGGQ"
     gene            3301..4206
                     /locus_tag="PLM79_00355"
     CDS             3301..4206
                     /locus_tag="PLM79_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HOV84783.1"
                     /translation="MKNVNGLKGDKRIRVFSTEDRQCVWMKAGVVNYKLCDQAFDCLH
                     CPFDKAMSRTKVRKPGAVSSWRRAMRTKPYKEKECRHMLTGRVQYHFCSNGYRCNVCE
                     YDQYLDEADLAEAMGTIHTSKIAGFTVAHNYYYHRGHGWARVEHGGFVRLGMDDFALR
                     LLGRPTEIRLPKLGSRLEQTEVGWSIRRDRESAEVLSPIDGLVVATNHKAVGDPHQVK
                     TDPYGAGWLIVVEPQGLKSNLKNLLFEREAAAWVKAEREKLEHMVMASQGFPLAATGG
                     EIVDDVFGNLPQMKWEDLVHEFLLT"
     gene            4642..4953
                     /locus_tag="PLM79_00360"
     CDS             4642..4953
                     /locus_tag="PLM79_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84784.1"
                     /translation="MALFLNYDFPGNVRELENVLEHACVLCKAPMIELRHLPVEFPAK
                     ARCAPPFTLTKTLLECSEEKTIPDLPTKHKGCRISAALEPGNKPLHPLAENPKIPAQD
                     A"
     gene            5150..5401
                     /locus_tag="PLM79_00365"
     CDS             5150..5401
                     /locus_tag="PLM79_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84785.1"
                     /translation="MTIAIPLFMRRASPNFSTAPELLIVQVRDQQICVRAKLDLSFVS
                     LLERRKKLLLLGVDILVCGGLDRGTLMWLEARGIRVMAV"
     gene            complement(6014..6901)
                     /locus_tag="PLM79_00370"
     CDS             complement(6014..6901)
                     /locus_tag="PLM79_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOV84786.1"
                     /translation="MKYFYYPGCSLEGTAAEYDVSTRAVLGKLGVELVELEDWTCCGA
                     SAAEAVSRLLSMVLPARNLALAQKAEVKADFLVPCSACYLNLRKVEEQVRQDGELLAR
                     INEVLEEEDLRYRGGIHSRHLLDVLANDIGADRVLGSRTLSLEGLRVVPYYGCQALRP
                     YQTYDDPEFPKSMEPLIEALGAEVVPWNLGAKCCGAGLMTTKKEAAQEAVAQILAGAR
                     GGDCIVTICPMCQMNLEGFQKKISKIFGEDLRISVLYLPQLIGLALGLSDRELKLDKN
                     LALHSELWDRLRSLKRGSP"
     gene            complement(6898..7476)
                     /locus_tag="PLM79_00375"
     CDS             complement(6898..7476)
                     /locus_tag="PLM79_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV84787.1"
                     /translation="MSGHTVQTSSGAGEPSHKDPLAPIGEWVRACMQCGTCTGSCVNA
                     DAMDPVPRQLWRMVQLGLKEEIFRSKTFWLCSSCYYCTLRCPRGLPLTETMAALKRIA
                     IRENLLPDKKSSRFYLSFMETVRRYGRVREMELMARYFLSLKDPLTPLSFTPLGVKLL
                     TRGKLAPQFPSLFGTGKLDAIYRKVQELEAGR"
     gene            complement(7489..10917)
                     /locus_tag="PLM79_00380"
     CDS             complement(7489..10917)
                     /locus_tag="PLM79_00380"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV84788.1"
                     /translation="MRKKVGSALVVGAGISGIRSALDLAEMGYGVTLIDRAPHLGGTL
                     RQLDLQFPNDHCGMCRMLPLVDRDASSQYCLRKGFFHENIQIRLSTELVGVEGDPGKF
                     KVTLRRQPTLIRTEMCVGCGECTKVCPVEVPDEFNAALGRRKAVYLPVPHNIPNRYVI
                     DPHACTRCGQCEKACPTGAVDLGLQARRGFRVLVVDDEFVIRDSMKEWLEVEGFSTDT
                     AESGFDAVDKLAANSYQLMLVDIKMPGMDGVEVLKRAKEMQPELEVIMMTAYATVETA
                     VEAMKVGARDYLVKPFDPEAVIAMVVKLFESTLPSPEAQMEVGAIILAAGFSSYDPRV
                     GANTYRYGELPDVVTGVEFERLVSGTGPNGGRLLRPSDGRQVGKIAWLQCVGSRNLQE
                     NADYCSSVCCMFSVKEALLAKEKAGYDLDAAIFYMDMRTFGKHFHQYRDAAEREAGVR
                     FVRSRVHSVDREDDSGNLRIQYTEPDGTMKEEEFDLVVLATGQRPSSGTEELAEVAGI
                     ERNPWGFCRVNGESLSRSSREGIFVSGSFSGLRDISESVIQAGSASLGASSLLHSKGG
                     SLAQVAVEETAYRDVSRDPPQVAVVLCTCADIWAQRVDWTAVQSHLSKDGAVARVLPM
                     ERLCTEQGWNALEESLKDPGWNRVLIGACQPYVYARKLRDLGKTIGLHPSLIEVVDTC
                     TPVFALGTDSGVDPTPAVRDVLNMGIAGLRTAEPPLPSTTPMNQKALVVGGGIAGMTA
                     ARALADHGYPVVLVEQQEELGGNLRHIHRTLEGPSPRQILEQTVAAVENHPQITVHKN
                     ARIIHSQGRVGRFFTTIGKENGLGETIDHGVTILAVGGLEAPTTSYGHGQSEAVVTQH
                     ELEAGLEAGKIRPSDLQVVVMIQCVDSREEGRNYCSRICCASALKNALHLKEQNPELD
                     CYILYRDIMAYGFLESHYTEARRRGIVFIQYHPSEKPSVRVENHRPHLSFRDPILNRD
                     VRMNPDLLVLSTGIVPTFPPQVAPIFGVEIDGDGFFREADSKWRPLDFLQEGIFTCGI
                     AQSPRSISESAASAEAAAQRALRLLSKEHVAAGTVVAEVRHTLCSLCERCVEACPYGA
                     RWVDEEEERIRVDEIACQGCGSCAAVCPNSASVLRGHRDQQVFAVLDALMDEVG"
     gene            complement(10945..12093)
                     /locus_tag="PLM79_00385"
     CDS             complement(10945..12093)
                     /locus_tag="PLM79_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOV84789.1"
                     /translation="MAKTAKIEVKEQNPVTSLRGFLQELLGGGQVDGLLVAAHLPVKG
                     SVMPVLVTDPQRMDGVDPLAPAFPINAAKVVSRLTRKPTGQRLAVVLRPCEIRAFIEL
                     VKLKQGSLDEVVLIGMDCLGAYGNVDYARLAGENGGQETTLRFYRDLPSNMRGALEDS
                     EISPACKACEHPVPENADLQVAFLGVPMDRMLLVQARTEKGMELFRKLRLTECEEPKE
                     RQAAVAELVEGRRAYRDRMFEETSRATADVEKLMEYLGRCVNCYNCRVACPVCYCKEC
                     VFVTDVFDHEPTQYLRWARRKGLLKMPTDTVFYHLTRLAHMSTACIGCGQCSNACPNG
                     VPVMELFRTIAAHTQRAFDYEAGRSAEEPPPLSVFREKEFSEVTGGKD"
     gene            complement(12105..12527)
                     /locus_tag="PLM79_00390"
     CDS             complement(12105..12527)
                     /locus_tag="PLM79_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV84790.1"
                     /translation="MGEHEPKIIAFVCNWCTYTAADLAGTSRLIQPPNVRMIRMMCTG
                     MVDPKYIIKALLEGADAVLVSGCHPGDCHYINGNLKARRRIKLLKEILPRFGLDERRL
                     KMTWIGASEGIDFAETIRGLVAEVKALGPNALRNQMVL"
     gene            complement(12546..15923)
                     /locus_tag="PLM79_00395"
     CDS             complement(12546..15923)
                     /locus_tag="PLM79_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOV84791.1"
                     /translation="MLKRIGFYICHCGTNIAGKVRTEEVVEFARGLQGVVVARDYKFM
                     CSDPGQEMIQKDIRELGLNRVVVASCSPRLHEKTFQGACGRAGLNPYYFQMACVREHC
                     SWVTEDPNEATHKAKSLAAAAINRVNFHQGLVRREVAVHPDVLVVGAGISGIQASLDI
                     AKAGHHVYLVERDPSIGGHMVQFDKTFPTLDCAACISTPKTVAVYQDPNIHLLTYSEV
                     VELNGFVGNFTAKIRRKPRYVREDRCTGCGQCADACPVSVPNRFDEFLSTRKAIYRYF
                     PQAVPITFCIEKKDRAPCTLTCPAGINVQGYVQLISRGKYREAVQLILEKVPFPGVLG
                     RVCPHPCEFQCRRVEVDVPLAIRDLKRFAADRTDLAQLPLPPISDRPEKIAVIGSGPA
                     GLTAAYYLRLKGYSVTVFEALDVLGGMLRVGIPDYRLPPEVLDREIDHVLRLGIGVRT
                     GKCLGRDFTLDDLRQEGFQAVFLGLGAHSSLKMNVPGEGELQGVIDAVEFLRRVNLGN
                     RRAPGKKVVIVGGGNVAVDAARTALRLGCETVTVVYRRSREEMPAYAEEIEGALEEGV
                     VIHYLTAPVGILGSEGRVTGFQCIRTELGPPDESGRRRPIPVAGSEFVIECDAVIPAI
                     GQEPAAQWIEAAGVQRSRRNTVLVNRHTMQTSVPGVFAAGDLVSGPATVIDAIAAGRS
                     ASDAIHAYLCGEDMDAYAASVEAQKPEGHDWREVPDHTAPESRAVLRHRPASECTGSF
                     DEVSLGLDEEEAAREASRCLNCGVCSECMECVRACERNAVDHDIREETLELKVGTVVL
                     ATGFDILDPTPLKQYGYGRFPEVYTGLEFERLNNAVGPTGGRILMKNGASPQSVGILH
                     CVGSRDHNYHEYCSRVCCMYALKYGHLIKEKVGHDTKVYNFYIDMRCFGKGYEEFYRR
                     LQEEGVTFIRGKPGEITRDALHPAEQGKLIIVSEDTLLGRKLRIPVDMVILCTAMEAR
                     KDASEVARVFGINQGGDGFFLEEHPKLGPLSTATDGIFLAGTCQGPKDIPDAVSHASG
                     AAAQALALATRGKVEIAPTTSWIDPDVCAGCRTCIQLCAYNAIEFNPRKGVSVVNEAL
                     CKGCGSCAGFCPSGAARIKHFTAKQVFAEIEGLLEAM"
     gene            complement(16761..19031)
                     /locus_tag="PLM79_00400"
     CDS             complement(16761..19031)
                     /locus_tag="PLM79_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV84792.1"
                     /translation="MSEKISLIRQSLTVQLILWVGVILLGSISVWAYANIRYHETNAM
                     GHMVEEADRLGNTIKLGAHYAMMLNARDDINQIILNIGRRPDIRIIRIYNKEGRIKFS
                     NLAEEVENSTDIRAEACIICHRGESPLETVPLGERTRVLKVGESERLLGIISPIYNEP
                     GCSEASCHYHPAGKKVLGALDVVFSLSRMDNEIRSHKEGIIALALFSFLGVASFITVF
                     FFFFVNLPIRNLIQTTRMIGSGLYRNPDGTNRKDEIGQLALSIQQMGREIAEKQETLN
                     KQREEYQRLFEQVPCCITVQDRELRIIQFNQEIDRRFNPTPGEFCYRAYKGRRDRCEE
                     CPVLQTFRDGENHVGEEVVLNRDGTESRWFVRTSPIRTAAGEVCAVMEISLDITEAKR
                     LEQEILKSERKYHGIFNNIPNAVFVVDGENLEILDCNESASALYGFDGKSMVHQSFLD
                     LFEEAEQGSYAGRLPSSRVLNQVRHITRRGETIFVDMHVSPTEYSERRALLVVASDIT
                     KRLLAEQQLIQTSKMATLGEMSAGVAHELNQPLSVIKTAGNYLLRKVQKGEDISPDIL
                     RTMAEEIDAYVDRAAKIINHLRDFGRKAEVTKQPVSVNDALSRAHEMFDQQLRLRKIR
                     VVKELAEDLPPVLADANRLEQVFINLLVNARDAIEQKVERTGRRDLDKEIRLKTRCEK
                     GWVFIEVADTGIGVPEHLRGKIFEPFFTTKKVGQGTGLGLSISYGIVQDYGGRIEVKS
                     EMDEGSTFVVRFPAAE"
     gene            complement(19372..20742)
                     /locus_tag="PLM79_00405"
     CDS             complement(19372..20742)
                     /locus_tag="PLM79_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV84793.1"
                     /translation="MKSPTTKSQRAGAGRSGCPELSPQTEIILNSIADGVFTINLDWQ
                     ITSFNRAAEKITGVSAVEAIGRPCCEVFRANVCETTCVLKYTLESRKPVVSKPIAILR
                     ADGKEIPISVSTALLKDAGGEIIGGVETFRDLSLVETLRKEIDKQYRFGDIVSKSPAM
                     QRLFSILPEVAQSESTVLVQGESGTGKELLSRAIHFLSPRAKGPFVAINCGALPDTLL
                     ESELFGHTAGAFTDAKKSRVGRFAAAEGGTLLLDEIGDISPALQVRLLRVLESHSYEP
                     LGSSKSVKADVRVVAATNRDLAKMVEEGSFRRDLYYRIHVIKLELPNLARRKEDIPLL
                     AEHFIDKMNKLKNKNILGLSHEALAIFMRHEWPGNIRELENVIEYAFILCREGLIQPK
                     CLPDYLRGQAEGCPDYGGLTLRDIEKRAIREALERNEWRRMATARELGIDKNTLRRKM
                     KRHNLA"
     gene            complement(21277..21645)
                     /locus_tag="PLM79_00410"
     CDS             complement(21277..21645)
                     /locus_tag="PLM79_00410"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOV84794.1"
                     /translation="MPGRDGTGPLGMGPMTGRGRGVCCGSPAPTPFWGGRGRGGGGFG
                     NRCRLWGRGPARPTGPGYGSPGWQGPGIRNRPGEPSSAAGPAQEETLQILTSQAQHLE
                     DALKDVRDRIQRLKTKTTED"
     gene            complement(21950..22858)
                     /locus_tag="PLM79_00415"
     CDS             complement(21950..22858)
                     /locus_tag="PLM79_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84795.1"
                     /translation="MVELVVISGKGGTGKTSILGAFAALASRKVLCDADVDAADLHLL
                     LSPRIGEREGFWAGYKAFIQPESCIRCGECRKKCRFEAVSEEFVVDEMLCEGCGVCAY
                     FCPEGAVDMRPNLCGEWFVSDTRHGPLVHARLGIAEENSGKLVSLVRKQARRVAEEKG
                     LDMILTDGPPGIGCPVIAAVGGATAVLIVAEPTVSGHHDLERAAGLARGFHVPVLVCV
                     NKADLHPPSADSIEQWCRGHGHQFVGRLPFDPVFTRAQLVGKTILEHGDGPARTAVME
                     LWDQVRSFLGSGLQPSFDREAFSGKS"
     gene            complement(22870..23718)
                     /locus_tag="PLM79_00420"
     CDS             complement(22870..23718)
                     /locus_tag="PLM79_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84796.1"
                     /translation="MIIAVASGKGGTGKTTVAVNLAMALPGEVQLLDCDVEEPNANLF
                     LRAAVTEREEVHMEVPLIDNQACTYCGKCREICRFNAVAIVPETRVALAFPELCHGCG
                     GCFAVCPEGAVLDSRRLLGVVEKGKRSTVKLVQGRLRVGEAMAPPLIRKVVAQAEETG
                     ITVVDAPPGTSCPVVTSLRRADCAVLVTEPTPFGLNDLELAAGAVRKMGIPAGIVVNR
                     CDVGDGRIRDFARREGIPILLEIPFDRAVAEAYARGELLVERFPRWKERMLHLHSRIQ
                     GLLVPE"
     gene            complement(24148..24372)
                     /locus_tag="PLM79_00425"
     CDS             complement(24148..24372)
                     /locus_tag="PLM79_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84797.1"
                     /translation="MAPGRLQDQRGQGRGGTGRGQGRQGGRGDGRTGPGGSCLCPKCG
                     YKEVHLRGVPCTQVRCAQCGHVMLREQTPT"
     gene            complement(24400..24828)
                     /locus_tag="PLM79_00430"
     CDS             complement(24400..24828)
                     /locus_tag="PLM79_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOV84798.1"
                     /translation="MSNSTKSMMEAIYRRVGKNPGQQPEKAAAGSRDQLGIMKRMEDA
                     SCAGKITGSCGETMEVFLKLDGDKIIDASFFSDGCWFSVLCGWVATQLARGKSVEEAV
                     EIGGDTVLSMFKEVPENEAHCAQLAAETLHAAVHDWMVKK"
     gene            complement(24844..26070)
                     /locus_tag="PLM79_00435"
     CDS             complement(24844..26070)
                     /locus_tag="PLM79_00435"
                     /inference="COORDINATES: protein motif:HMM:NF041136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140132 - iron-sulfur cluster carrier
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein MrpORP"
                     /protein_id="HOV84799.1"
                     /translation="MSSCHSGQCGHEPKSSEEKQLQEQLRHIGHKLLVMSGKGGVGKS
                     SVATYLSIGLSRLGYRVGLLDVDLHGPSIPRMLGVEGMFRVNDGKRMVPHTYGDKLQV
                     VSIQCLLDDPDSAVIWRGPVKHGVIQQFISDVDWGELDYLIIDSPPGTGDEPLSVAQT
                     MPDAKAVIVTTPQEVALADVRKSINFCRKVEMPILGLVENMSGYVCPHCNAELPLLGK
                     GGGARTAARMKVRLLGSLPFDPRVVEAGDVGRSLLDSPDGSPFVKAVGELVLEVVGLC
                     GNPSFQSDPKPEKVAPPDLPEHFKVAVPLADGRLTNHFGHCEQFALLDVKEGRIEGKE
                     LLTPPPHEPGVLPRWLGGLGVNLILAGGMGKRALDLFEEQNIRVITGAPNLAPEELIH
                     QYLNGSLTTGANVCDH"
     gene            complement(26313..26987)
                     /locus_tag="PLM79_00440"
     CDS             complement(26313..26987)
                     /locus_tag="PLM79_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84800.1"
                     /translation="MAQRIPFGFNKEASEIAGEGIFLIAIPVFRGRVAPVLDWCSTIH
                     LYPEVSGSETPEQELFLTHATTFDRMRILRVKGVRTLICGVLSPDLLHYGQRMGIRII
                     HGVAGEVREVLRAYLGQKLDHPRFRLPGCKGPRNYREGCKGVCAADAAAESCPSLERR
                     KSRGRQGGEGGRNRGLETGAKGKIAAGPGGSCLCPECGARIPHRNGIPCGQVPCPACG
                     RSMVRG"
     gene            27393..27755
                     /locus_tag="PLM79_00445"
     CDS             27393..27755
                     /locus_tag="PLM79_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOV84801.1"
                     /translation="MKIAISSSAGNLDAEVDPRFGRAACFLLVDSETMDFVVLENQQN
                     YQAAQGAGIQAATLVARHKPAAVLTGHCGPKAFEVFRWAGIPVVVGVSGRVRDAVDKF
                     LGDGFTPASSADVAGHWG"
     gene            complement(27793..28506)
                     /locus_tag="PLM79_00450"
     CDS             complement(27793..28506)
                     /locus_tag="PLM79_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84802.1"
                     /translation="MEDDLVASLTRQVKEEVIENYLNERKLVHLQMEELEQRAEKVRC
                     LAVETGKRLCRVGLLLINPEMLERLRKLLCISPSSYWNACFNSHFPRTVRTIRVRGFT
                     LKARYRKLFLEAFQRFQYWMESYRKEYEDLSLECRAVNCNIQGFQKNFDLLSLLSFLK
                     SLDIQGLERKHFLGENFTPGELAAVERSLCLCPVSFEKMAVPEPLCLPGYQLLEESLI
                     ALAHEVYQRFPGQVQRLMH"
     gene            complement(28547..>29095)
                     /locus_tag="PLM79_00455"
     CDS             complement(28547..>29095)
                     /locus_tag="PLM79_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84803.1"
                     /translation="HGFPVLHVCIDETPEKIKIWYQELLRNILGPGHHRDVPRIQQQI
                     EPLRFIHAYLHHTFSPGKLDQSVRNLRDQANFQPAMVVLDGLDFDRISRTSVEELKEL
                     AGKHGVSMWMSARMHRHMSTTNARGIPYPCHEMDGLFEAILLLEPVPDVIQVKVLKHE
                     GQYLPQRPAVLLNPQTYLLQRR"
     assembly_gap    29096..29195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<29196..31245)
                     /gene="fusA"
                     /locus_tag="PLM79_00460"
     CDS             complement(<29196..31245)
                     /gene="fusA"
                     /locus_tag="PLM79_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOV84804.1"
                     /translation="MVEDLSKIRNIGISAHIDSGKTTLTERILYYTQRIHAIHDVKGK
                     DGVGATMDFMELEKERGITIQSAATHCTWADHPINIIDTPGHVDFTIEVERALRVLDG
                     AILVLCSVGGVQSQSVTVDRQMVRYRVPRLAFINKCDRTGADPFRVVRQLKEKLNHNA
                     VPLQVPVGLEGDHEGVIDLIAMQAVRFEGDHGSVVTRTEIPAELMDLAMKHREEMLDA
                     VSLYSDELTEAILEERVTEDLIHAAVRAGTLSLQITPVLMGSAYKNKGVQPLLDAVCR
                     YLPSPMDKSNEALDLDRDEAKVDLFNAPDLPLVMLAFKLEVGRYGQLTYVRLYQGTLR
                     KGDTIVNVRSGKKTKVGRLIRMHADQMEEIESAGAGQIVALFGVDCVSGDTFTDGRVH
                     YTMTSMHVPTPVISLALKPRDKKAEINMSKALARFTKEDPTFRIIMDEETGETIICGM
                     GELHLDVYVERMKREYQSDVETGMPKVAYRETITRRSDFNYTHKKQTGGAGQYGRIAG
                     YIEPYPDGDFQFVNQITGGAIPTEFIPSCEKGFQACLSKGSMAAYPIVGTRVVINDGA
                     SHSVDSSDLAFQQAAIGAFREAYAKAGPILMEPLMRVVVETPSEFQGMVLGTLNQRRG
                     MIVSSSEDAAFSTIEAEVPLAEMFGYSTVLRSTTQGKAEFTMEFARYSPVPRGIAEEI
                     V"
     gene            complement(31418..33346)
                     /locus_tag="PLM79_00465"
     CDS             complement(31418..33346)
                     /locus_tag="PLM79_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HOV84805.1"
                     /translation="MLGPNDVLGITASVGQPYHGRLVNGVPFPNQFQGYTLRDQERSY
                     TTPEVVGAVLDAVERVREQHPGTCDLFVGDFSVQGGGWLNHHRSHQNGRDVDLGMYLK
                     GNRAADTFVSMNEDNLDVPKTWCLVEGLLRSQRVQYIFLDRRIQRLLHDYAASRGADS
                     AYLERIFGGGFGRSVINHVANHHDHIHVRFYTPWSTLAAHVAGMDEQKRTVIEMAQQA
                     FLPKKVNYYVKGNEKSLESLARSFGVQYRDLCRWNQLSGNSPISPGSCLVFYKRGFEL
                     EPVHLAQALSPNFEAPGVHLAALRPVRPETPTPPPDSVSDAVEEAPAAVVRHVPREKR
                     VEPPSTASYTVQRGDTPASIARKNKMELRALLQMNGFKKVPQLKPGQKLKVTAGRSSG
                     SVTAASSTSSGFGRHDISTRQTVMTDASRGKGTTTVTYTSVKGDTLAKISRKSGIPLD
                     ALCQLNAMKRNAPLKPGQKIRLAQGATMSKGMGPTSEPGRMSDRGPKAVSKSPDKKAA
                     VGKADPKKNLSKVKAVPAKPVAEPKNGASKKVAVQGKDAPQGKKAAATKGVNPAKNGA
                     QASGKSVGKTAPESKAASSATAAKTSASPNAKSKAGSSSKEKVSASVKKPAPAAPGNA
                     KGNTVTTAKTSKQVATRR"
     gene            complement(33754..35559)
                     /locus_tag="PLM79_00470"
     CDS             complement(33754..35559)
                     /locus_tag="PLM79_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOV84806.1"
                     /translation="MAERTSTGFGILGIVLVSLILLPCFLNPPDLAAQETRPSQEPLS
                     SPQGQSRVAEDTASRWRAALWEAVPAELLSDSGPGTLVDVYGQNDWKPLFFDFRFQLA
                     PGTALFLKRLEDLESDAVNPASFPLDSLKKGLKEVEELRAALQPTPAANQDRTGANPP
                     GAGQEASPSPPPSQASVASQPAPANGETDRLSDVERDPRYLQLLKVASETDVKLGACL
                     IRFAQEMNPFSPELREKVLLGKMSMAEFLEAVVPNSPHYATLRKALTKYRSLAAQEPW
                     HRFHSGKTLRQGDSGQEVSNLQKRLAQEGFYSGKISGHFDHATADAVRKFQRYHLIDA
                     DGAVGQRTKDWLNVPYNKKVRLLQESLKQLRQSRARPYDRYVRINIPQFMLEYYRDGK
                     IQAEHRVIVGKAAGKKVKVQGRWMGENQTPSISSVIDQVVFNPRWYVSDRIRRELADE
                     IAADPNYLDKHGYVKMSSNYPWGEPRLFQLPGPGNPLGRVKFEFSNAYAIFLHDTPKR
                     HLFQRAKRDFSHGCIRVEKARELAQLLLADDGNSAVGKTESFLESYTRQTFVKFNQPV
                     PIIIEYVPASSDGNGQVVFCGDLYGWFAEEGTQRS"
     gene            36308..36877
                     /gene="tadA"
                     /locus_tag="PLM79_00475"
     CDS             36308..36877
                     /gene="tadA"
                     /locus_tag="PLM79_00475"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOV84807.1"
                     /translation="MDSPSRQAAPASGQHGTRDGLPTVAAFGKEAILSKEHHRWMGVA
                     LEEARSAYGESEVPVGAVLVSKDGELLARAHNAPLGLCDPSAHAELLVLRQGARVLGN
                     YRLPGTTLYVTLEPCVMCYGALLHARVRTLVFGAYDSKSGALGSAVDLTTVSVFNHYI
                     EVVPGVRAAESAELLRRFFQERRSEREQA"
     gene            36879..36974
                     /locus_tag="PLM79_00480"
     tRNA            36879..36974
                     /locus_tag="PLM79_00480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36914..36916,aa:Ser,seq:cga)
     gene            37019..37112
                     /locus_tag="PLM79_00485"
     tRNA            37019..37112
                     /locus_tag="PLM79_00485"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37054..37056,aa:Ser,seq:gga)
     gene            37176..38957
                     /gene="dnaX"
                     /locus_tag="PLM79_00490"
     CDS             37176..38957
                     /gene="dnaX"
                     /locus_tag="PLM79_00490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOV84808.1"
                     /translation="MPYLVLARKWRPQTFDEVIGQPHVTQILRNSVESQRIAHAYLFT
                     GSRGVGKTSVARILARCLNCEKGISSLPCNECSNCQEITQGNSMDVIEIDGASNRGID
                     NIRELRETVRYRPGKSRFKVYIIDEVHMLTTEAFNALLKTLEEPPEHVLFLMATTEPH
                     KIPATILSRCQRFDFRRIPMDLTVQHLKKIVGREHTRLSDSVYYAVAREADGSMRDAQ
                     SLLEQLLAFSGEDLTDEEILDLLGVVDRRSVHRLGRAILEGDLPTCLRVAADLYRRGI
                     DSRRVCQQLCDHFRNLLFFSLAETEGSVQPDLPEDEKGILREDVRKTSTESLYLYIQM
                     LLKGEEEIRRSTLPKMALEMLLVRLAQLPRLSSLEQLMGRIAQLEQALEGRDSEPSEP
                     RRASGCTPSVPPVAPPAVSSEPLAPLEISMPPGGVDSPAPPAPQRAVRAEEAAQRWSS
                     FLEWIQDRDPVLGAKLQNSRVLPNPDGTLTLEVLELFKDRVSEPQTLANLSEATLTFF
                     SNPFQWKVEVRPTPSADSPDRPRSRKTRINHTRLVMENPVFQDALEILGGELLDIKPL
                     RTGSGKRTGVDPGSGSPDGDFLGNDSP"
     gene            38992..39318
                     /locus_tag="PLM79_00495"
     CDS             38992..39318
                     /locus_tag="PLM79_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOV84809.1"
                     /translation="MVKGFNPMQQVKALQEKMARMQEEMAQKTVESSAGGGMVTVRVN
                     GRQELLDLKIDPQVVDPHDIEMLQDLIVAAVNDALRKSQEMVAGEVGKLAGGLSIPGL
                     KIPGLF"
     gene            39423..40025
                     /gene="recR"
                     /locus_tag="PLM79_00500"
     CDS             39423..40025
                     /gene="recR"
                     /locus_tag="PLM79_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOV84810.1"
                     /translation="MNAYPPILRKLIGILSKLPGLGEKSATRIAMHLLQASPGEAESL
                     AETIQEMKKRIRTCPRCFLVTDSELCGICADPARNTGEICVVETMADLLAIEGSGSYK
                     GRYHVLEGALAPLDAVGPENLRVAELLKRLPEENIREVILATNPSNEGEATAHYLSRL
                     IKSHKVRVSRIAFGIPMGGDLKYTDRVTLERSLKGRQAFE"
     gene            40345..40971
                     /locus_tag="PLM79_00505"
     CDS             40345..40971
                     /locus_tag="PLM79_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84811.1"
                     /translation="MNRARIKYTASAMVFLAVLAFVVPSYAARQHNAGGGGRHHISSN
                     RPMNSNRAINRGPVNRGYRNQVHANRYRGQMGDFRLRNAARAAGPSNKQYSRRAYDNK
                     RHWNGRHYDRYRYGYNGRYWDGRHGYNHGRYWNGRYGYNHGRYWDGRHHHHYWRGNRY
                     YYYGGWGGPYWGGYYGWGDGWPYYYWSSYYYYPFPFFYFPGFSFMFYL"
     gene            41024..41746
                     /locus_tag="PLM79_00510"
     CDS             41024..41746
                     /locus_tag="PLM79_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84812.1"
                     /translation="MIKKVTLLGLGFGLIAAGIAGISMSVVEKKGKDALKQMEKLQQE
                     NQSAAAEEWKKIEEKRQEVEKLAAGVEAEKRRVREEQRRAQEELRAREANLARKKLEM
                     PGTKAASSQTAGEKGKKGKATKTTLASKDSGKKQGTAAASGRSRSVKASKARDEDGFR
                     WISRKAGAEAARISRPVKYHNLRTRELVLAEPLDYGPHSVRVRIRVWKDQRLIKDTTI
                     TISEDALRGPGPTRHGRGRDFA"
     gene            41795..42241
                     /locus_tag="PLM79_00515"
     CDS             41795..42241
                     /locus_tag="PLM79_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84813.1"
                     /translation="MPLSAMIFLCSFAILVGAVLVAYGLAQEFRTRESVGAEKCPPFA
                     GEGKVPIDEPLVLDFKEEFSVKEPADAEPSQTPGWSARKNKRTLGEIASGFSASVLGG
                     LRKVLRSPPPEPGTAPKTDLDHPSPTGTVIGAKTLPGSPEIARREG"
     gene            42697..43647
                     /locus_tag="PLM79_00520"
     CDS             42697..43647
                     /locus_tag="PLM79_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOV84814.1"
                     /translation="MRDAAIHEQCKIEVDVRRQYREEIPGVVEALVQSCRVETCFDHV
                     GPEPIPSRETVIDIIHRVRRILYPGYFTRERLDGFNLEYYLGQEATALFEGLSEQITR
                     ALRHDCIRHNLPCVECEALGQQAAVGFMGRLPELRNILATDVRAAMEGDPAAGSYDEV
                     IFSYPGLLAVTVYRVAHELLRLKIPLIPRIMTEYAHSHTGIDIHPGARIGESFFIDHG
                     TGVVIGETTEIGDRVRLYQGVTLGALSLPKNAVESLRDQKRHPTIEDDVIIYSGATIL
                     GGDTVIGARSVIGGNVWITESVPPDTKVFLKQPELVYKGA"
     gene            43716..44654
                     /gene="cysK"
                     /locus_tag="PLM79_00525"
     CDS             43716..44654
                     /gene="cysK"
                     /locus_tag="PLM79_00525"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOV84815.1"
                     /translation="MVGRIFEDVSKTVGNTPLVKLNRIAREADAVILAKIESFNPLSS
                     VKDRIGVSMIEDAEARGLLHSDSVLIEPTSGNTGIALAFVAAARGYRLILTMPESMSV
                     ERRQLLAVLGAELVLTPAAEGMTGAVNRAEKLLLEIPHSVMLQQFTNPANPEIHRRTT
                     AEEIWNDTDGKVDILVAGVGTGGTITGVAEVIKERKKDFRAVAVEPESSPVLSGGRPG
                     PHKIQGIGAGFVPGVLNRGIIDEIIRVKDEDAGRIARRLARLEGLLVGVSSGAACWAA
                     LELAKRRENRGKMIVVVLPDTGERYLSTWLFQEPNS"
     gene            complement(44723..46042)
                     /locus_tag="PLM79_00530"
     CDS             complement(44723..46042)
                     /locus_tag="PLM79_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV84816.1"
                     /translation="MAESPEASGVGFLFRSLHSRNYRLFFIGQGVSLIGTWMQQAAMS
                     WLVYRITGSTLLLGVVAFSSQIPTLVLGPVAGVVADRWERKRILIWTQCLSMVQALIL
                     AAVVLGGAVRTGHILALSVFIGIVNAFDIPVRQSFVVQMVERKEDLGNAIALNSAMFN
                     SARFIGPSIGGILISTLGEGICFLLNGISYLAVLAGLAAIRIAPRPIRNGKAPVWREF
                     YEGLRYVFEFKPILAILALLSIFSLAGAPYLVLLPAFAKDVLCGGAHTYGFLMSAAGI
                     GALAATMYLASRRSARGLIRLIPSAAAACGLGIAFFALSGNVALSIVCLFTAGFGMMI
                     QIASSNTILQTIVDENKRGRIMSLFAMSFLGVMPFGSILAGSVAERIGARATLLLGAA
                     SCITGALIFAVKLPGLCQQLRPFFQEPEIRETDPSKSRTRLHSKPNR"
     gene            complement(46109..47023)
                     /locus_tag="PLM79_00535"
     CDS             complement(46109..47023)
                     /locus_tag="PLM79_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84817.1"
                     /translation="MKRILGCAVLCLLLLSGTALQAAEPLVLYDNFKANLINPSKWFG
                     VEKFDAPFFRENLRRVEQHSTLGNQLRLMSRAYAGMGSDSGDEKGGNRLVFTRPDGIK
                     AMKATFWVNGYTSKGCAGNSVPANARARLSGFFFNTGTPTQWSSQHDVMAQIFIQRKS
                     DSKDAPDTLRVVGTIVECQDDNCYTFTPLDTRQLGTIKVGSKVTLYIEWDKANKRFIF
                     QRDTKPQVFSTYTVSDSSAPGTVFNKRIDVQLNVPNCTSSPRPTAHMDVYVDDVYVNQ
                     SALSAPVSLALEEDALRANVCMGDGNGP"
     gene            complement(47460..47840)
                     /locus_tag="PLM79_00540"
     CDS             complement(47460..47840)
                     /locus_tag="PLM79_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84818.1"
                     /translation="MATMDDPEERRREVPGAFTGLVFVVFGLYFHIAFFGIHSLLTAR
                     AFLFLVPGMYVTAILVGVPFYVVRGGILNLTLRLLKEPVNRSHPSMLKCITTASFVLE
                     MVVVCLLSKKAFLWFYAGSNLRGL"
     gene            complement(48066..48641)
                     /locus_tag="PLM79_00545"
     CDS             complement(48066..48641)
                     /locus_tag="PLM79_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV84819.1"
                     /translation="MLTEAFAHHPVLPRDTPPETRRALLELFIKTFAGSNRAWLHGIR
                     RDGTLVCAAFSLDPRFKPGPKAMLRFFGGLLCLLGWRTGWEFMTSLSRRPKYDLPYLE
                     LLLLGTLPTFHGQGLGRTMLRFLYGFCGTLGYHGVILDVAQDTPAYEFYVREGFVVDG
                     QITMNKMLLCHMRRADAERECTQESRGGATR"
     gene            complement(49058..50215)
                     /locus_tag="PLM79_00550"
     CDS             complement(49058..50215)
                     /locus_tag="PLM79_00550"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HOV84820.1"
                     /translation="MNTKTIFMRVMIGFLVLVLAAPAGTFAQGSGSAGPYRQEELDQM
                     LAPIALYPDSLLAQILMAATYPAEVAEADLWLKHNRNLTENQLNDALDEKDWDLSVKA
                     LVPFPQVLALMAEKPEWTKKLGDAFMAQEGQVMDTIQSLRAKAKAKGNLKSTKEQKVV
                     VRGESIEIVPANPRVVYVPTYNPLEVYGPWWYPAYPPYVYSPFYPVGGIVTAGVFGFA
                     AGIAVGSAWNWGWGRWDWGHRDIIVNVNRNININRRDIRINDVRTASWNREMKLKHDP
                     SYRRQAAKEKQQKQAITQKQKKQDQQLKQKQKRQDQQLKQKQKKQDQQLKQKQKRQDR
                     EGQPKAQKQKQQREKQQMRQKEQRQEQRMRQKQERQRQHESQKEERQRHGR"
     gene            complement(50837..51940)
                     /locus_tag="PLM79_00555"
     CDS             complement(50837..51940)
                     /locus_tag="PLM79_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84821.1"
                     /translation="MEHPYLLFPHLLLREEDLRALCLLLPRLEIFQVLRGPAVPSYAW
                     EKVRSRPTIHDPEDLERIGLYLKGYLDFAERQGETGLLVCRSRSTLAGEEEESRFQIR
                     GRIKEQESDEEDLKKRCLLEAAVFLETARNLDERESEIASGLLEAQKLEDEFRRILGI
                     EPSETTEEAMEVITPSLGSGNADFSYMILKRASCWYRLLSQGPAEPGWVPAIFSRQLL
                     EEILEPLESEREKRGEDAPPMEVPLCGMPSPGLLSPEAFGSLQEELDRDGIRESFWKA
                     LDGFLDDPEDPLLLETLVREGSALTTRMEQWCEPRRGSGGSRVRLSLVRLKGCTHGDL
                     WRSLDREGFSELGSEADFLDREALFLCLDRDSP"
     gene            complement(52059..52904)
                     /locus_tag="PLM79_00560"
     CDS             complement(52059..52904)
                     /locus_tag="PLM79_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOV84822.1"
                     /translation="MTRTLSLGYSPCPNDTFIFGALAEGRIDPSPLAFKIFMADVEVL
                     NRKAGAGELDVTKVSVSAVVRLLEDYWMLRAGGAMGRGCGPLVVSRAALSPGELRRRS
                     LAIPGKLTTAHLLLRLEGSHQGPVFEMPFDQIMPRTVAGEVDAGLIIHEGRFTYPSMG
                     LKSVLDLGSWWESRWGVPLPLGAILIRRELGPEIARTVEERIRRSLNHARNHPEEVWP
                     YIRDHAQEMEPSVIERHIEMFVNEFSLDVGEEGERAVRTLLREASLLENIPLPNKPLF
                     WDQED"
     gene            complement(52913..53752)
                     /locus_tag="PLM79_00565"
     CDS             complement(52913..53752)
                     /locus_tag="PLM79_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84823.1"
                     /translation="MTGVHKFHVAVLGAVPAEVEGFFDLLEGSRSLTVAGRPFLAGNH
                     RGRPVLIGTTGLGKVNAAITTAALLQGLSVSEVWNVGTAGCYVDSPLNLGDVLVTETA
                     WCGDEGVLTREGVLSSREIGIPVLEVGGRECFDSIPLDAQPLMERIRARTPPGCYTLD
                     VGSMAAGSRAAPCKSASPHPVLGTPTGCSPVSFRIVYGPSLTVGMVSGDEETARLRSE
                     RFSVYAENMEGSAVIHACLRFGVPVLECRGISNTAGDRFKDRWQTEKALSHCRAVVGT
                     WLS"
     gene            54174..55241
                     /locus_tag="PLM79_00570"
     CDS             54174..55241
                     /locus_tag="PLM79_00570"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOV84824.1"
                     /translation="MHDQEQTAPFRPPPLKIGPLVVDPPLVLAPMAGVTDSVYRWIMA
                     EHGVGLVFTEMVSAQGLLRGNRESVRLCRQDLPLPGPLSVQIFGGDVDVVGRAAASLE
                     ALGASAVDINAGCPVRKVLRQGAGAGLLQKPDLLARMVEKVKRSVRIPTTVKIRLDWT
                     QSYSATVNLAKRLESAGADAITLHGRTPAQQFRGDADWTAIEQVKKSLGIPLIGNGDI
                     TRPSQADRVLREGKCDGVMIGRGSLGNPWLLSTIVSRWRSPVQRNPHPGWEDFFRTVH
                     DHVRAFREKWPHCMGRLRTLLMYYSKGFPDTCRLRARLSTADGWDVLLSAFDSWMEDI
                     ARRGWAFLPFKIPELKKGEEP"
     gene            55238..56980
                     /gene="ispH"
                     /locus_tag="PLM79_00575"
     CDS             55238..56980
                     /gene="ispH"
                     /locus_tag="PLM79_00575"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOV84825.1"
                     /translation="MKVIVAKTAGFCKGVKDALEVTLNAIQQRKEGERICTFGPLIHN
                     RQVLKMLEEKGIQEVADIENCAGKKVVIRAHGIPPRERQALHRMDARLLDATCKRVAK
                     VHAVIKRHARRGYRTVIVGDADHAEVIGLMGYTEGRGIVIDRVEQVEQLPRDWEKVLL
                     VAQTTQNEETFREITEAFLKRYPKGIVKNTICGSTHERQAEVREMCSQVEAMVIVGGY
                     HSGNTVRLVEVARECAIPTYHVETEADLNQQDMERYSCVGISAGASTPNWMIRSVVHY
                     LESIQTRKMSRKYLLKRAMELLAYGNLYIAVSAALLPSAMQAMTGFPGSWYDGLMAAF
                     YAFAMHSLNIYLDRNAIKLNDPSRAAFYQRWRLAFTGAGVAAVVAALWIALDVGFLAF
                     LVMGVLVLLGISYAVPVFLPSSWQSLYAFKLKDIPTSKTFSVPIAWASVTVIVPYTAH
                     ALPGLDRAIFAFWIAFLMVLVRTAMLDLLAVQGDRLVGRETLVVLIGEAKTGHFLVGT
                     LILLGLSLVVGPWAGLCTSFSYVMLPAVALYWSQLNMCFQNRLKEDPAFVSAIESTLI
                     GVGLLGLLWNLVVQ"
     gene            57041..57748
                     /locus_tag="PLM79_00580"
     CDS             57041..57748
                     /locus_tag="PLM79_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HOV84826.1"
                     /translation="MREFDRAELEQFDGKDGKPAYILHRGRVFDVSASKLWKGGFHMR
                     RHHAGTDLTVDLHAAPHGEEVLDRYPQVGILGEKGPAPRSASGLLSFLLERYPMLRRH
                     PHPMTVHFPIAFLMAVPTFTVLSLLTGIEGLETTGFHCLGAGLLFLPVVMVTGIFSWW
                     LNYQARPVKAIGIKLAASLVLLVLALISFVGRSSGGETLASLGPGGVFYVLGVLAFVP
                     LVVTIGWYGAGLTFPTE"
     gene            57946..59739
                     /locus_tag="PLM79_00585"
     CDS             57946..59739
                     /locus_tag="PLM79_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV84827.1"
                     /translation="MADDFRLPNQEELEKELSEYLSKKYGYRIKVISPMMMAQAKESP
                     DTPTPSEPTGVDKIRFDLKPEELAAYLDQYVVKQDGAKAVLATKICTHYHRIRFEKKR
                     GRAEEASMVGRIKNNILLIGPTGVGKTYLVKLIARKLGVPFVKGDATKFSETGYVGGD
                     VEDLVRELLVEAKEDIELAQHGIIYVDEIDKIASSHSLIGPDVSRTGVQRALLKPMEE
                     TEVDLKVAHDPISQLQAIEQYRKTGKRERRVINTKNILFIMSGAFNELAPLVKKRLQR
                     QEIGFGAEISSKGDDYRYMKHVKAEDLIAYGFESEFVGRLPVVAVLEPLGVEDLYEVL
                     RNPNNPVLLAKKEDFRSYGIDIRFEDRALRLLARMGYEEKTGARGLVSVIERVLLPFE
                     KSLPSSSVKTLVVTEGLVIHPEEELKRVLANPEDPDRLAVYRAILEEEKGRARVQLSE
                     THNHYAQEYPLIFTGPRMEIALEHHIRFGWSIESVLDEMMQLYNQVRVFEGDFCETHG
                     FQISFDEEAVDEIIQEALNTGTSATGICARVSRDYDYGFKLVSERSGQLRFVLPRDAV
                     LQPDQYLDDLIRDSYRQYPITLSDRQQESQN"
     gene            59751..60929
                     /gene="ahbC"
                     /locus_tag="PLM79_00590"
     CDS             59751..60929
                     /gene="ahbC"
                     /locus_tag="PLM79_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HOV84828.1"
                     /translation="MIGISKLYCGTVEPSDALRYGRLSGRLPSHLLQFSTDKQPVVVW
                     NVTRRCNLKCVHCYAHAKDQPFENELSTEEGKRVLDDLSEFGCPVILFSGGEPLVRPD
                     LPELAAYAVQKGMRAVISTNGTLITPAVAAELKSIGLSYVGISLDGMEEVNDRFRGVK
                     GAFQRALMGIRACREEGIKVGLRFTMNRLNGFEIPSIFDLLEEEDIPRVCFYHLVYAG
                     RGSKLMEEDLSHEETRAAMDLIMDRTRDLHERGKSKEVLTVDNHADGPYVYLRLLREG
                     SPRAASVLELLRMNEGNNSGRGIGCISWDGRVHADQFWRHYSFGNVRERPFSRIWTDL
                     SNPLMKRLKNKKEYVKGRCARCRWLDVCGGNFRVRAEAAVGDLWLPDPACYLTDEEIR
                     "
     gene            60947..61918
                     /gene="hemB"
                     /locus_tag="PLM79_00595"
     CDS             60947..61918
                     /gene="hemB"
                     /locus_tag="PLM79_00595"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOV84829.1"
                     /translation="MNFPIYRPRRFRRTENLRRLIRETTWSVDQLIYPLFAVPGKGVL
                     NPVQSMPGVFQRSVDELLREVKTVVDLKIPGVLLFGLPESKDEHGSQAHSREGVVQEA
                     VARIKDKFPDLLVITDVCLCEYTSHGHCGILRGHEVDNDATLDVLSQVALSHARAGAD
                     VVAPSDMMDGRVGSIRTCLDENGFSQVGVLAYSAKYCSAFYGPFRDAADSAPQFGDRR
                     AYQMDFSNAGEALREVALDIDEGADMVMVKPALAYLDVIRRVKDEFGWPVAAYNVSGE
                     YSMIKAASANGWLDETRVMMEILTAIRRAGADCIITYFAKDAARILS"
     gene            61942..63057
                     /gene="ahbD"
                     /locus_tag="PLM79_00600"
     CDS             61942..63057
                     /gene="ahbD"
                     /locus_tag="PLM79_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HOV84830.1"
                     /translation="MNHPWGKNHESKKAHPSGHASGRAPALRLVAWEVTRTCNLACVH
                     CRAAAQDRPYENELTTEECLGLLDNIATFADPIIILTGGEPLLRPDILEIAAYGTQKG
                     FRMTMAVNGTLLTPDKARRLKEVGIRRISVSLDGATSASHDAFRQVPGAFEGALRGIE
                     NAKAEALDFQINTTITRQNHSELPAIQELAVSLGATAHHIFLLVPMGRGKDIAQQGID
                     AEQYEKTLHWFYDQRDRVPLRLKATCAPHYYRILRQRAKAEGREVSPQTFGLDAMTRG
                     CLGGTGFVFVSHVGRVQPCGYLELDCGNVREKTLSDLWHHSPIFRDLREFRKYEGKCG
                     GCEYVRVCGGCRARAYESTGSYLAEEPLCLYRPGQKT"
     gene            63140..63643
                     /locus_tag="PLM79_00605"
     CDS             63140..63643
                     /locus_tag="PLM79_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV84831.1"
                     /translation="MDNVPGKNNDVLCRAVRQSLGVLVLAVLIGLSGNALRHDGLPWI
                     GDWSPPAQLVRSGVPEGMIIALDEAAALFHAGGAVFIDARPEEAFRAGHIAGARNLPW
                     QDFENRFSEAMSDLPPDSILITYCDGESCSLSKDLSLALLGKGYPFARILVNGWTVWK
                     EAGLPAE"
     gene            complement(63742..64899)
                     /locus_tag="PLM79_00610"
     CDS             complement(63742..64899)
                     /locus_tag="PLM79_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_268815321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(64639..64641),aa:Sec)
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOV84832.1"
                     /translation="MTFLRKSCAFLLWLLLTSAAYAAGPPVNPPPPSAPAPTQPQEAP
                     SLSVPAPVFDFGEIVEGEVVVHDFSIRNTGTDALQIDQVRPGUGCAVAHFDRAVPPGG
                     EGKVTLRVDLKGYQGNVKKTATVFSNDPQNPRVVLTVQGFVRSLIEVRPGSAVAFRGV
                     ADQLAEKTIELSSTTETFNVGSVETNLQDKIQYSLETVQEGKHYQLKVRNRLKQGTYS
                     GFVKIRTDHPRKPEILIRVNGSVEGEIGVRPPSVLIGKLVPHQPPRTGKVMVVSNKDR
                     PFQITKLTYDESLLRVTRQPLPKDAGFSLEIVPLLENMAPGSRTQLVLTIETDVEPQE
                     EHEVHVQLLHIADPPAAPSRNGSKGSEKATGSSPRNSSGDQGPPRSEEKGK"
     gene            complement(65095..65934)
                     /locus_tag="PLM79_00615"
     CDS             complement(65095..65934)
                     /locus_tag="PLM79_00615"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOV84833.1"
                     /translation="MAAKPQLPPAAASRPLEKAPPPPQPVVPPLPLARAAIVIDDLGQ
                     DLQIAKRFLAVPLPLTYSILPYQQHSREIAHLARYHQREVLLHLPMEPKGYPKTRPGP
                     GALLVSMSESAVRQTLRKALDETPFAAGVNNHMGSRFTERAELMGVLFAELRQRHLYF
                     LDSYTTEKSVCIALASQYGVPFLRRRVFLDHENSEAFVRAQILALIRKARVEGSAVAI
                     GHPREATLKALLDMAGRFQEEGVLVVPSGELLPRGPSRQSPTGKEDPNPEDLQRGSSA
                     GAS"
     gene            complement(66313..67635)
                     /locus_tag="PLM79_00620"
     CDS             complement(66313..67635)
                     /locus_tag="PLM79_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOV84834.1"
                     /translation="MSPKQKRLLLFLLGSAILLGCWSTLRAKPVADSPDIYQYLRTFS
                     DVLNIVQDNYVDKVDSKKLIHGAINGMLRELDPHSSFMKPEDYKELQIETKGKFGGLG
                     IEITIRDGVLTVVSPLEDTPADKAGILAGDQIIKIDDQLTQDMTLMEAVQKMRGPKGT
                     KVKLSIIRKGERKPLEFDLVRDMISIRSVRSRTLEPGYGYVRISSFQSGTANDLRKAL
                     EQLETGSQPLMGLILDLRNDPGGLLDQAVEVSDEFLDQGLIVYTGGRLESQQMRFEAH
                     KNGKPHNYPIVVLVNSGSASASEIVAGALQDHKRAIILGERTFGKGSVQTVIPLNDGS
                     AIRLTTSLYYTPSGRSIQAKGIEPDIVVKRETPAKESEEVPDDPRRVREKDLPRHMEG
                     QKSDPKDPKASEALAEVQKMLEQDNQLRRGLDLLKGYKIMAQMQMQSK"
     gene            complement(67645..68577)
                     /locus_tag="PLM79_00625"
     CDS             complement(67645..68577)
                     /locus_tag="PLM79_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOV84835.1"
                     /translation="MFLLLILGGVDRYGWFPRASEQEPELSREKAGKSDFRTQQAAET
                     ARKRLVWFYKYGRIGYLFPLLAASEPGDFPENVHRVSRMMRRDREIFARVAERPGEIH
                     GIVESLDGHAREERLKDLPAEKKPTPGPPGEGIGKGDPVSKTRSGHPDRREPGASPSN
                     GTAEGRDPFGSPFAAMQKALPLPTPGELKRTFGKSPGASKKFLYNNGTLILAPKGQPV
                     VAVHDGVVVFADWVKEYGQVVIVNHGDHYFTLLAHLDQVFKGVGEPVQGGETIALVGD
                     SGSTDGSKLYFEIRQGGRPLDPKDWLAAHTTMKE"
     gene            complement(68616..69524)
                     /locus_tag="PLM79_00630"
     CDS             complement(68616..69524)
                     /locus_tag="PLM79_00630"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOV84836.1"
                     /translation="MLERRYRYLAQSSLRGVGGNWFASSAALAALTLCFFLWGLLAAG
                     ALALRQSLPLWWTSDKATLYLLPESSEEEQQRLIRFLKGASEIAEVRILSSQEAKKRL
                     EHQLGGWKQILSGIEDPILPTTIEIAFKPKVRHGEQIEAVLDRVRRQPAVEEVFYGKS
                     WVERIESATSGLRLAGTGLMYLLALGIFFIILHAVKSSVESRREELEAYEILGATFLF
                     GKVPFYVQGFLLGSAGALMGGVPAGLLVLRAEKVMPLPISGMVTLDLWKGVLLMLGLI
                     LTGAVLGVGGGWLGARLYGPWGRVSS"
     gene            complement(69580..70374)
                     /locus_tag="PLM79_00635"
     CDS             complement(69580..70374)
                     /locus_tag="PLM79_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV84837.1"
                     /translation="MTESCPSTKSKIENGETPRTTGAFSWERTADHEPSGTGAFSMSR
                     AMVQCLHLHKSHPGTGPILQNACLEIGKGEWAFLVGETGSGKTTLLRLLTGLEPVSRG
                     QVIFAGKNLHRASPKERAAVRHAMGLVPQEPKLVSWQTVSQNIALPLYVSGKDNALIR
                     ERVQRLTTLLNLQPRKDVLCSRLSAGEKQVVSVARALAGDPLLLVADDPTAALGPLAV
                     LNVLDVLKFFHARGTTILIATRDRTLAQQVPGSRVFALQGGEIVEE"
BASE COUNT        13727 a        21063 c        21104 g        14492 t
ORIGIN      
        1 acggggggcg tcagcccgta tttcttgatc ttgttgtgca gggtgatcct gctcacctcg
       61 agaaccccgg cggccttgga gatgttccaa ccgcactgct tcagaatgtg gtcgatgtgt
      121 tgcttttcgt tttccttcaa ggattgggga accgtccggc ccccggttcg caggaggaag
      181 gcaaaatcat cccgggtgag atggcggcct ttggcaagaa ccaccgcccg ctccacggcg
      241 ttttgaagct cccggacatt gccgggccac tcatagcggg cgagcagctc cattgccggg
      301 gattggattc ccatgatggg cttgttggtt tccctccggg acctttccag gaaatgctgg
      361 gcgagaacgg gaatgtcttc ctttcgaagc cgcaggggtg ggacttcgat ctcgatgacg
      421 ttgaggcgat aatagaagtc ccgtcggaac accccgcgtt cgatctcctt gggcaagtcc
      481 cgatgggtgg cgcagatgag tcggaaatcc acggagattt ccttggttcc tcccacccgg
      541 tggaaggtct tctcttcgag gaccctcaaa aggtccacct gcatcttcaa cgggatttcc
      601 ccgatctcgt ccaggaagag cgtccccccg tgggccattt ccaggcgccc cttcctggct
      661 cgaacagccc cggtgaaggc accgctttcg tgaccgaaca actcgctttc caggagggtt
      721 tccgggaatg cgccgcaatt gatgggaatg aacgggctgt aggaccgtcc gctcctggaa
      781 tggatcgccc ttgccaccag ttccttgccc gttcccgttt ctccgcgcag gacaacggga
      841 gaatccaccg cggccacttc ctcgatcaag tcgaagagtt gctgcatgca gggggagacc
      901 cccaccaggt cctcgcagcg aactcgggtg gccacatgct cgcgcaggag gaggttttcg
      961 tcgaggatct tcttctgctg aaggagcttt tccacgagga gggccaggtt ttcgggttcg
     1021 aagggcttca tgagatagtc gttggcgccg cttttcatgg cctccaccgc cgtttgcacc
     1081 gatccgtagg cggtaatcat gaccaccatg ctttcgggat agttggcctt gatctcgtgg
     1141 agcacctcga gtccgtccat gtcgggcatc ttgatgtcca ggaagatcag atcgaactcc
     1201 cgttccccga gcatctccaa ggccctgcgc ccgccctcgg ccccggacac ctcatggccg
     1261 gatttcttca gccagcccac gagagactcg cgcacgatca gttcgtcgtc cactaccaga
     1321 atcttgatgc ggcctgtcat cctgcatctc cctgtggttt cctgttccgg ggcggaagcc
     1381 tattgaccgc ccccctccgg gtcttcaagg gcaaagaaac ggatgatgaa ggtcgagccc
     1441 ttgccttcct cgctcttcac atagatggat ccctggtgtt ccttaatgat tccgtagacc
     1501 accgacagcc ccaggccaac ccccttgtcc tggctcttgg tggagaaaaa gggctcaaaa
     1561 atatgggaaa tcatgccctg gggaatcccg gctcccgtgt cctcgatttc gagccggatc
     1621 aggttggagg acgcttccca acaggtcctc accgtaaccg ttccgccctc gggcattgct
     1681 tccaaggcgt tgaacaggag gttgatcagg cactgctgaa tctggccgga atctccgtac
     1741 atacggggca acccttcggt cagggccaat tccagccggg caccctggag ctgcatccgg
     1801 tggctgaaaa gagccgccac cctccggacc acatcgttga gctggaaata cccctgctcc
     1861 atcttctttt gccgggcaaa agagagcagg tttcccacaa tggtgctgca tcgagccgac
     1921 tcggtatcga tgagatccag gtagtattgg tattgtttca ggtcctccgg gctgggggga
     1981 ccggcccgga gaccctggga caccaggcgg gcgagggcat gaatccccgt gaggggattg
     2041 ttgatttcat ggactgcgct ggctaccagc tttcccaggg cgatcatctt gtcctcgtgg
     2101 atcaaccgag caaggttcat ggtgaggatc cgcgtcttct gctccacccg cttgaccagt
     2161 tcatcggtaa tgtccctcat gatctcgaga aagagctcga tcttaccctg tttgttcttg
     2221 agggggacga tggtgacctc acaggtacgg gtttcgttgt ttcggtcaaa atgttcgtga
     2281 atggcgtgag cggtccgtcc cgtctcgagg ctctccttca aggggcagtg atgccccttc
     2341 tcgtcgcacc gcgaaaccgt ccagtggctt atctcgtagc agcgcttgcc cacgatgtcc
     2401 tgcctggtcg cccccaagga ctggaggacg gcttcgttgg catccaggat tttgaagtcc
     2461 ggctggagga tgagtacccg atcctggatg cttgaaaaga tgctgtccac gatgccctgg
     2521 atcagctcga gctggcgctt ttccaggagg tattgctcct ggaagcgaat catgtcgctg
     2581 aaaagccgcg agatggtggc ttccaggacc cgggtgttct tgggtttgtg ccgcagaaaa
     2641 tccaggagca gcgtttcgtt tcccgtgagc tcgatgacga ggtccaggtc cttgattttg
     2701 aaaaagtcat tgtagtcccg ggtggtgaaa gtccctcttt cccgggcgat gcgcatgccc
     2761 acggcgtctt cattggggtc cgccaccgcc acgattcggg cctcgaggtg aggaaagcgc
     2821 atggcatcga gcatctcgag gaaagcggca cacctctttc ctcccccgat taccgcgatg
     2881 ttcatcacct tgttggcttt cacttcccct acccccctca taagaggtgc ataatttccg
     2941 aacagggcgg aaaaaatcct tacactgggg ttaaatcgtt gccgggggag gatcgaacgg
     3001 gtccccgaga cccgaggccc tttgaaagac ctgccttcga gtccctcctc cggcgagatc
     3061 cacccgggca ctgctccctc gagaccccgg gttcgctccg gttcgttgac ggagtcgtcc
     3121 gattctttcc gttccttctt cccttgttgc gcccatcccc gtctcgacct tccatcccct
     3181 cacggcattg ggcagaaccc ttcttccagt cttccggctg atgaatgtcg tgccggcatg
     3241 aatcttggtt accttccctt tgcaagacct caaccatcca acaaaggagg atgatccgcc
     3301 atgaaaaacg tcaacgggct caaaggcgat aaaaggatcc gggttttctc cacggaggat
     3361 cgacaatgcg tgtggatgaa agccggagtg gtcaactaca aactatgcga ccaggccttt
     3421 gactgtctcc attgtccttt cgacaaggcc atgagccgga cgaaggtgcg aaagccgggg
     3481 gcggtctcca gttggaggcg agccatgaga accaagccgt acaaagaaaa ggaatgtcgc
     3541 catatgctga cgggcagagt gcagtaccac ttctgctcca acggctaccg atgcaacgtc
     3601 tgtgaatacg accaatacct ggatgaggcg gacctggcgg aggcgatggg gacgatccac
     3661 accagcaaga ttgcgggttt taccgtggcc cacaattact attatcaccg gggtcacggg
     3721 tgggcaagag tcgagcacgg cggctttgtt cgcctgggga tggacgattt cgccctgcgg
     3781 ttgttgggac gccctacgga aatccgtctt cccaagctgg gctcgcgttt ggaacagacg
     3841 gaggtggggt ggtccatccg gagggatcga gagtccgccg aggtgctttc ccccatcgac
     3901 gggctcgtgg tggccacgaa ccacaaagcc gtcggggacc ctcatcaggt caagacggat
     3961 ccctacgggg ccggctggct gatcgtcgtg gaaccccaag gacttaaaag caacctcaag
     4021 aaccttcttt tcgaacggga ggcggcagcc tgggtgaagg ccgaaaggga aaagctggaa
     4081 cacatggtca tggcctccca agggttcccc ctggccgcca cgggtgggga aatcgtggac
     4141 gacgttttcg gaaatcttcc ccagatgaaa tgggaagacc tggtccacga gttccttttg
     4201 acctgaggga gccccgaccg gatgtccggc cccaacatcc aattcctcga tgatcgggag
     4261 tgaggacgag aacgaggtgc cgctcccgat cttcccttgc cgaggagtcc gggaagaagc
     4321 ggaggagggc tcaattgtga ctcccgtgcc cctgcgccgt cttgaattcg gctcctcgaa
     4381 gagaggcaat tcgttcccct cgggggggcg gtccccgtca aggccgatgt ccggatcgtc
     4441 gccgccggca accaatgcat cgaaagcctg gtccaaaggg gcgcctttcg agaagacctg
     4501 ttctatcggt tcaacatcat tcggatccgg ctgccccctc ttcgtgatcg aaaagaggac
     4561 atcccgcttc tcaccgattt cctgctctcg aaactgaact ccctccgccg caggaggatc
     4621 tccggcgtct cgggggaagt catggccctg ttcctcaatt acgactttcc cggaaacgta
     4681 agggaactgg aaaacgtcct cgaacatgcc tgtgttttgt gcaaggctcc catgattgag
     4741 ttaaggcatc tccccgtgga attcccggcc aaagcccgat gtgctcctcc cttcaccctg
     4801 acgaaaaccc tgttggaatg ttcggaagaa aagacgattc cggacttgcc cacaaagcac
     4861 aagggctgca gaatctccgc cgccctggaa ccggggaata agccgctcca cccgttggcg
     4921 gaaaatccga aaataccggc tcaagacgca taagagaatg ctttccggcg ttttgcccgt
     4981 gaaacagtct ctgtgaaaca gtttcaactc tgcaacaacc ctcgcgcttc ctgtcttgcc
     5041 tcttccggat aacagtccgt tttccctcta tttccaggga tcaaaaaggt tcgaggagcc
     5101 ggccatgtgc cttgccgtag aacccttgtg tccgacacag ggacggcaaa tgaccatcgc
     5161 gattccacta ttcatgaggc gagcgtctcc caatttctct acggcgcccg aactgctgat
     5221 cgttcaagtc cgggatcagc agatctgtgt gcgggcgaag ctggacctgt ccttcgtctc
     5281 actcctggaa aggagaaaga agctcctcct cctgggggtg gacattctcg tctgtggagg
     5341 gctggatcga ggcaccctga tgtggttgga ggcgcgcggc atccgagtca tggccgtgtg
     5401 aggggcgagg tgtcggaggt cctctctcgg ctcctgggcc tgtagcagct ccccgtcttc
     5461 gggaaccgga aaggacctcg atcctgccga cgagaaagca cttgcgtgcg aggaaggagg
     5521 atcatcatca agaagaactt gggaacatac ctcttgtgtc tgatgctggg tcttgcggta
     5581 ccggctttgg gagcctgcac aaccgtaccc cttcagactg ccgcgaagaa acccgggtac
     5641 tcgcagcaag cggcccgggc ggcaccctcg cggcaagcgg aagggttcac cgccgaggtg
     5701 gattccgcga tcatcccgga ctagggatcc tacctcggct ccctgggagc cgcagattcc
     5761 ttcccgacta ccctgaagtg cgcggggaag atcggccgtg agaggattcc ggggggtcgc
     5821 cgcttcccca tgaagaccgc gggcatggag gaaactgacg gcctcccggc ctctcatgcg
     5881 aggccgggtg cttagcatgg tgcccgtgtg ccgcgaggga cggcggggag ccgttcgttc
     5941 cccggaggag cctcccgccc gagggggaag ggcgaacgga accacgggac aaggaagctt
     6001 tcgccccgtg gttctaagga gacccccgct tgagggaacg aagcctgtcc cacaattcgg
     6061 agtggagggc caggttctta tccagcttga gctcgcggtc ggacagtccc agggccagtc
     6121 cgatgagctg gggaaggtac aggaccgaaa ttcggagatc ttccccgaaa atcttggata
     6181 tctttttctg gaaaccctcc aggttcatct ggcacatggg gcagatggtc acgatgcaat
     6241 cccctccccg cgccccggca aggatttgcg ccaccgcttc ctgggccgct tccttcttgg
     6301 tggtcatgag acccgccccg cagcacttgg cccccaggtt ccagggaacc acctcggccc
     6361 ccagggcctc gatgaggggc tccatggatt tggggaactc gggatcgtcg taggtctggt
     6421 acgggcgaag ggcctggcat ccgtagtacg gcaccactcg aagcccctcg agcgacaggg
     6481 tccggcttcc caggaccctg tcggctccaa tgtcgttggc gagaacatcc aacagatggc
     6541 gggagtggat tcctccgcgg taccggaggt cttcttcttc cagcacttca ttgatgcggg
     6601 ccaggagttc tccgtcctgt ctcacctgtt cctcgacctt tcgaaggttc aggtaacagg
     6661 cgctgcaggg aaccaagaaa tccgccttca cctcggcttt ctgcgcaagg gccaggttcc
     6721 ttgcgggaag gaccatggac agcagccggc tcacggcctc ggcggcactg gccccgcaac
     6781 aggtccaatc ctccaactcc accagttcga ccccgagctt gcccaggacg gcccgggtgg
     6841 aaacgtcgta ttccgccgcg gtaccttcga gggagcagcc gggatagtag aagtatttca
     6901 tcggccggcc tccagttcct gcaccttccg gtaaatggcg tccagttttc cggtcccaaa
     6961 aagcgagggg aattggggag caagcttccc cctggtcaga agtttcaccc ccaagggagt
     7021 gaagctcagg ggtgtcagag ggtccttgag ggatagaaaa taccgggcca tcaactccat
     7081 ttctcgaacc cgcccgtatc gacgcacggt ctccataaag gagaggtaga accgggagct
     7141 tttcttgtcg ggcaggaggt tctcccggat ggcgatccgt ttgagtgccg ccatggtttc
     7201 ggtaagggga agaccccgag ggcaacgcag ggtacagtag taacagctgg aacagagcca
     7261 gaaggtcttg cttcggaaga tctcttcctt caagcccagc tgaaccatgc gccaaagttg
     7321 ccggggaacc ggatccatgg catcggcatt gacgcaggag ccggtacagg tgccgcactg
     7381 catgcaggca cggacccatt cccctatggg agccaggggg tccttgtgag agggttctcc
     7441 tgcgccgctg ctcgtttgaa ccgtatggcc gctcatgttt tctcccttct agccgacctc
     7501 gtccatcagc gcatccagca cggcaaagac ttgctggtcc cgatgacccc gcaatacgga
     7561 cgcgctgttg ggacacacgg cggcacagga accgcaaccc tgacaggcaa tctcgtcgac
     7621 ccggatcctc tcctcctctt cgtccaccca gcgtgctccg taagggcagg cctccacgca
     7681 tctctcacac agagagcaga gagtgtgacg gacttcggcc accacggtac cggccgccac
     7741 gtgctccttg ctgagcagtc tcaaagcccg ttgggctgcg gcttccgccg aagcggcgga
     7801 ttcggagatg gaccgggggg attgagcaat cccgcaggta aagattccct cctggagaaa
     7861 gtccagggga cgccatttgg agtccgcttc ccggaagaac ccgtcgccgt cgatctccac
     7921 gccgaaaatg ggagctacct ggggcgggaa tgtggggacg attcccgtgc tgagaaccaa
     7981 caggtccggg ttcatccgca cgtcccgatt gaggatgggg tcccggaaac tcagatgggg
     8041 tcgatggttc tccacccgta ccgacggttt ttccgaagga tggtactgga tgaagacgat
     8101 gccccggcgc ctcgcctcgg tgtaatgact ttccaggaac ccgtaggcca tgatgtcccg
     8161 gtacaggatg tagcagtcca gctccgggtt ttgttccttg aggtgcaggg cgtttttgag
     8221 agcggaagcg caacagatcc ggctgcagta gttgcgtccc tcctcccgcg aatccacgca
     8281 ctggatcatg accacgacct ggagatccga ggggcggatc ttgcccgcct cgagcccggc
     8341 ctcgagttca tgctgggtga cgacggcctc gctctgcccg tgcccgtacg acgttgtggg
     8401 cgcttcgagt cctcccaccg ccaggatggt gaccccgtga tcgatggttt cccccaggcc
     8461 gttttccttc ccgatggtgg tgaaaaaccg gccgacccgg ccttgggagt ggatgatccg
     8521 ggcgtttttg tggacggtga tttggggatg gttctcgacc gcggcgacgg tttgctccag
     8581 gatttgccgg ggggagggtc cctccaaggt ccgatggatg tgccggagat ttcccccgag
     8641 ctcctcctgc tgttccacca gcaccacggg atatccgtga tcggccaggg ccctggcggc
     8701 agtcatcccg gcaattcccc ctcccaccac cagggccttt tgattcatgg gcgtcgtgga
     8761 ggggagaggc ggctccgccg ttcggagccc ggcgattccc atgttcagga cgtcccgcac
     8821 ggccggggtc ggatccacgc cgctgtccgt tcccaaggcg aaaacgggag tgcaggtatc
     8881 caccacctcg atgagagagg gatggaggcc gatggttttt cccaggtctc ggagtttgcg
     8941 tgcatagacg tagggctgac aagccccgat caagacccgg ttccacccgg ggtcctttag
     9001 cgactcctcg agggcattcc atccctgttc ggtgcacagc cgttccatgg gcaacacccg
     9061 ggccacggct ccgtctttgg acagatggga ttgcaccgcc gtccaatcca cccgttgagc
     9121 ccaaatatcc gcgcaggtgc aaaggaccac ggccacctgc ggggggtccc gggaaacatc
     9181 ccggtaggcg gtttcctcga ccgccacttg ggccaggctt cctcccttgg agtgcaggag
     9241 agaagatgcc cccaaggacg ccgatcccgc ctggatgacg gattcggaaa tgtcccggag
     9301 tcccgaaaag gacccgctca cgaagattcc ttcccgagaa gaccgactga gggattcccc
     9361 gttcacccgg cagaaccccc agggattgcg ctcgattccc gccacttcgg ccagctcttc
     9421 ggtccctgaa gaaggtctct gacccgtggc cagaaccacc aggtcgaatt cctcctcctt
     9481 catggtgccg tcgggttccg tgtattggat tcgaaggttt cccgagtcat cttcccggtc
     9541 cacggaatgg acgcggcttc gcacaaatcg aacccccgcc tcccgttccg ccgcgtcccg
     9601 atattgatga aagtgttttc caaaggttcg catgtccatg taaaagatgg cggcatccag
     9661 gtcatacccg gccttttcct tggccaaaag ggcttccttg acggagaaca tgcaacagac
     9721 cgaggagcag tagtcggcgt tttcctggag gttccgggaa cccacgcact ggagccacgc
     9781 aatctttccg acttgcctgc cgtcgctggg acgcaggagt cttcccccgt tggggcccgt
     9841 gccgctcacc aggcgctcaa actcgacccc cgtcaccaca tcgggcagct ccccgtaccg
     9901 gtaggtgttg gcccccaccc ggggatcgta ggacgagaac ccggccgcca ggatgatggc
     9961 ccccacttcc atctgcgcct ccgggctggg cagggtgctc tcgaagagtt tcaccaccat
    10021 ggcgatcacg gcctcgggat caaacggctt taccaggtaa tcccgggctc ccaccttcat
    10081 ggcttccacg gccgtttcca ccgtggcgta ggccgtcatc atgatcactt cgagctcggg
    10141 ctgcatctct ttggcccgtt tgaggacttc cacgccgtcc atgcccggca tcttgatgtc
    10201 cacgagcatg agttggtagg agttggctgc tagtttgtcc acggcatcga acccggattc
    10261 ggccgtgtcc gtggagaaac cctcgacttc gagccattcc ttcatggaat cgcgaatgac
    10321 gaattcgtcg tcgaccacca ggactcggaa tccgcggcgg gcttgcagcc ccaggtcgac
    10381 ggctcccgtg gggcacgctt tctcgcattg cccgcaacgg gtgcaggcgt gagggtcaat
    10441 cacataacgg ttggggatat tgtgaggcac cggcaggtac acggctttgc gacgacccag
    10501 ggcggcgttg aattcgtcgg gaacttccac cgggcagacc ttggtgcatt ccccgcatcc
    10561 gacgcacatc tcggtccgga tcagggtcgg ctgccgacgc agggtgacct tgaatttccc
    10621 cgggtctccc tcgaccccca cgagctccgt ggacaaccgg atttgaatgt tctcgtggaa
    10681 aaaccccttc ctcaggcagt attgagaaga ggcgtcccga tccaccaggg ggagcatccg
    10741 gcacatgccg caatggtcgt tggggaactg gaggtcgagc tgcctcaggg ttccccccag
    10801 gtggggtgcc cggtcgatga gggtcacccc gtagcccatc tccgccagat ccagggccga
    10861 acggattccg ctgatgcctg ctcccaccac cagggcggag ccaacttttt tcctcatggg
    10921 ctgcgacctt ctatattttg gatcctaatc ctttcccccg gtgacttcgg agaactcctt
    10981 ctcgcgaaac accgaaaggg gcggaggttc ttcggcgctt cgaccggctt cgtagtcgaa
    11041 ggcccgctgg gtatgagccg caatggttcg aaagagttcc atcacgggca cgccgttggg
    11101 gcacgcgttg gaacactgac cgcacccgat acaggcggtg ctcatgtgag ccagcctcgt
    11161 gagatggtag aaaacggtat cggtgggcat cttcaggagc cccttgcgcc gggcccagcg
    11221 caggtactgg gtgggttcgt gatcgaacac atcggtgacg aagacgcact ccttgcagta
    11281 acagacgggg caggccaccc ggcagttgta gcagttgacg caacggccca ggtattccat
    11341 gagcttttcc acgtccgccg tcgcccggga agtctcctcg aacatgcggt cgcgataggc
    11401 tcgacgccct tccaccaact ccgccaccgc ggcctgcctt tctttgggtt cttcgcactc
    11461 cgtcagccga agctttcgga aaagctccat gcccttctcc gtccgggcct ggacgagcag
    11521 catccggtcc atgggaaccc cgaggaaggc tacctgcagg tcggcatttt cggggaccgg
    11581 gtgctcgcag gctttgcagg cgggagatat ctccgaatcc tcgagggccc cgcgcatgtt
    11641 cgagggcagg tctcgataga accgcagggt cgtttcctga ccgccgttct ccccggccag
    11701 gcgggcatag tccacgttgc cgtaggcacc caggcagtcc atgccgatga gcaccacttc
    11761 atcgaggctc ccctgtttga gcttcaccag ctcgatgaaa gcccggatct cgcaaggtct
    11821 cagcaccacg gccaaccgct gtcccgtggg tttgcgggtg agcctcgaga cgaccttggc
    11881 ggcgttgatg gggaaggccg gggccagggg gtccaccccg tccattcgct gaggatccgt
    11941 taccaggacg ggcatgacgg acccttttac ggggaggtgg gcggccacca gcagtccatc
    12001 cacctgcccg cccccaagaa gctcctgaag aaaacctcgc aaggaggtga cggggttctg
    12061 ttctttgacc tctatcttgg cggtcttggc catgttcttc tccctcagag gaccatttga
    12121 tttcgcaatg cgttgggacc caaagccttc acttcggcaa ccaatccccg aatggtctcg
    12181 gcaaagtcga ttccttcgct ggcgccgatc caggtcattt tcagcctccg ctcgtccagt
    12241 ccgaatcgag gaaggatttc cttgagcagc ttgatgcgcc gccgggcctt gaggttcccg
    12301 ttgatatagt ggcagtcccc gggatggcag ccgctcacca gaaccgcatc cgctccctcc
    12361 agcaaggcct tgatgatgta tttggggtct accattccgg tgcacatcat tcgaatcatg
    12421 cggacgttgg gaggctggat gaggcgcgag gtgccggcca ggtccgcggc ggtgtaagtg
    12481 caccagttgc acacaaatgc gatgatcttt ggttcatgtt ctcccatgtg atcctcccat
    12541 ggatgttaca tggcttcgag gagcccctcg atttcggcaa atacctgttt ggcggtaaag
    12601 tgtttgatgc gggcggcgcc gctgggacaa aatcccgcac agctgccgca ccccttgcac
    12661 agggcttcgt tgaccacgga cacccccttt cggggattga actcgatggc attgtaggcg
    12721 cacaactgga tgcaggtccg gcacccggcg cagacgtccg ggtcgatcca ggaggtggtg
    12781 ggggcgatct ccacctttcc ccgggtggcc agggcgaggg cctgggcggc ggcgcccgag
    12841 gcgtgggaga cggcatcggg gatgtccttg gggccctggc aggtccccgc cagaaaaatc
    12901 ccgtcggtgg ccgtggaaag aggccccagc ttggggtgct cctcgagaaa aaagccgtct
    12961 cctccctgat tgatcccaaa tacgcgggcc acttcggaag cgtctttgcg ggcttccatg
    13021 gccgtgcaca ggatcaccat gtctacggga atccgcagct ttcttcccag cagggtgtct
    13081 tcggaaacga tgatgagctt tccctgctcg gcgggatgca aggcgtcccg ggtgatctcc
    13141 ccgggttttc cccggatgaa cgtcacgcct tcctcctgga gcctgcggta gaactcttcg
    13201 tatccttttc caaagcagcg catgtcgatg tagaagttgt agacctttgt gtcgtggccc
    13261 accttttcct tgatgaggtg accgtatttg agggcgtaca tgcagcacac ccgggagcag
    13321 tattcgtggt aattgtggtc ccggctcccc acgcaatgga ggatcccgac gctctggggg
    13381 gaagcgccgt tcttcatgag aatccggcct cccgtaggac ccacggcgtt gttcaggcgc
    13441 tcgaactcga ggccggtgta cacctcgggg aatcgaccat acccgtattg cttgagggga
    13501 gtggggtcca ggatgtcaaa tcccgtagcc aaaacgacgg tcccgacttt gagctccagg
    13561 gtttcctccc ggatgtcgtg gtccacggcg ttgcgttcgc aagcccgcac acattccata
    13621 cactcggagc aaaccccgca gttgaggcac cgggaggcct cccgtgccgc ttcctcctcg
    13681 tcaagtccca gggagacttc gtcgaaggac ccggtgcatt cggaggccgg acggtgccga
    13741 agaaccgccc gggactcggg ggccgtgtga tcggggacct cccgccagtc gtgtccctcg
    13801 ggcttttggg cctccacgga ggcggcatag gcgtccatgt cctcgccgca gagataggcg
    13861 tgaatggcat ccgaggcgct gcgcccggcg gcgatggcgt cgatgaccgt ggcggggccg
    13921 ctcaccaggt ctcccgccgc aaaaacgccg gggacggaag tctgcatggt gtggcgattg
    13981 acgaggaccg tatttcgccg ggaccgttgg acccccgcgg cctcgatcca ttgtgccgca
    14041 ggttcctggc cgatggcggg gatgacggcg tcgcactcga tgacgaattc cgaccccgcg
    14101 acggggatgg gccttctgcg gccgctctca tcggggggcc ccagctccgt gcgaatgcac
    14161 tggaagcccg tcacccgtcc ctccgagccc aggatcccca cgggcgccgt cagatagtgg
    14221 atcacgaccc cctcttccag ggcgccttct atttcctcgg catacgcggg catttcctcg
    14281 cgactgcgcc gatacacgac ggtgacggtc tcacagccca gacgcagcgc cgttcgagcg
    14341 gcgtcgacgg cgacgttgcc cccccccaca atcaccacct ttttcccggg ggcccggcgg
    14401 ttgcccaggt tcacccgccg caggaattcc acggcatcga tcactccctg caactctcct
    14461 tccccgggga cattcatttt gaggctcgag tgggctccca gtcccaggaa aaccgcctga
    14521 aacccttcct gcctcaaatc gtccagggtg aagtccctgc ccaggcactt gccggtgcga
    14581 accccgatcc ccaggcgcag aacgtggtcg atttcccggt ccagcacctc cggcgggagc
    14641 cgatagtcgg ggattcccac ccggagcata ccgcccagga cgtccagggc ttcgaagacg
    14701 gtcaccgagt accctttgag ccgcaggtag taggccgcgg tcaggcccgc gggacccgat
    14761 ccgatgacgg cgatcttttc agggcggtcc gaaatggggg gcaagggcaa ctgggccagg
    14821 tccgtccggt ccgccgcgaa ccgtttgaga tcccgtatgg ccaggggcac gtccacttcc
    14881 accctgcggc actggaattc gcagggatga ggacagaccc gacccaggac cccgggaaag
    14941 gggacctttt cgagaatcag ctgcaccgcc tcgcggtact tgccccggga gatcagctgg
    15001 acgtagcctt ggacgttgat gcccgccggg cacgtaaggg tgcagggggc ccggtccttt
    15061 ttctcgatgc agaaggtgat gggaacggct tggggaaaat agcggtaaat ggccttgcga
    15121 gtgctgagga actcgtcgaa ccggttgggc accgaaaccg ggcatgcgtc ggcgcattgg
    15181 ccgcaccccg tgcaccggtc ttccctcacg taccggggtt tgcggcggat cttggccgtg
    15241 aagtttccca cgaagccgtt tagctccacc acttcgctgt aggtgagcag gtgaatgttg
    15301 gggtcctgat agacggccac ggtcttgggg gtggagatgc aggcggcgca atccagggtg
    15361 gggaaggtct tgtcgaattg aaccatgtgg ccgccgatgg aggggtcccg ttccaccagg
    15421 tagacgtggt gtccggcttt ggcgatgtcc agggaggcct gaatgcccga gatgcccgct
    15481 cccaccacca gcacgtccgg gtggaccgcg acctcccggc gaaccaggcc ctggtggaag
    15541 ttcacccggt tgatggcggc ggcggccagg gacttggcct tgtgggttgc ctcgttggga
    15601 tcttccgtca cccacgagca gtgctcccgc acacaggcca tctggaagta gtaggggttc
    15661 agcccggccc gcccgcaggc tccctgaaag gtcttttcgt ggaggcgggg ggaacaggat
    15721 gccaccacca cccggttgag cccgagctcg cgaatgtctt tttggatcat ttcctggccg
    15781 gggtccgagc acatgaactt ataatcccgg gcgaccacga cgccctggag accccgggca
    15841 aactcgacca cctcctcggt gcggaccttg ccggcaatat tggtcccgca atggcagata
    15901 tagaaaccga ttctcttcag cattctgaaa cctcttgggg ttgtgcggtc acccgccgtt
    15961 cgggaaccgg ggccttgttt cacccccggc catgaacgcc ctcgacccct gcagtcgggg
    16021 gcggaacgcc cgagccttgc cgttccgtgg tgtggttcgg gcatgattcc ggtctattgg
    16081 agacgatggg aagaggatct ccgccgtcac gcgttgtgcg gggcggatgg gggaagaggc
    16141 aagttccatg atcggtcttc ttccgcatac gccggccgtc tctcgggagc tacggtgggg
    16201 gttcgcggtt cccgggagag ttgggggtcc atgaagactg acttcgttcc agcgagaggg
    16261 tcaagattcg atgtcgggtg tcaggttcag gaggagggct gccgtttcga ggcccggcgg
    16321 gggccgattc tggaaaccga ttgtgagatt ccaggggaga tcgggattcc tgcacatggt
    16381 cattatgcat attcagtgct ccggggtcta gatgaaactt ttcacaatat gaagggttgc
    16441 ttgtcattcc ttcgtccaaa gggacgagca ggtgaaagga tcatggggtt ggccggtggg
    16501 ttccgcctcc cccgcatcct ttcgttttcg acgaaaccca ccggacccga tcacccggcc
    16561 gaatgccatc aaacaaaggc cgtgccaaaa ggaatgcagg gaattttgat gaggaattca
    16621 caaggagacg gggatcgtgt gatttttgcg acaaggttca ttatgtcccc aagcggtcat
    16681 tcgatggaaa cggatggttc ccatgcaggg agttcacttc gcccccaacg aaaaggaccc
    16741 ttcccaggct ggcacaagct tcattctgct gcgggaaacc gaaccacaaa cgtggatcct
    16801 tcgtccattt cacttttcac ttcaattctt cccccgtagt cctggacaat cccgtagctg
    16861 atggaaaggc ccagccccgt tccctgcccc accttcttgg tggtgaaaaa gggctcgaaa
    16921 atctttcccc tcagatgctc gggaacgccg attcccgtgt cggccacttc aatgaacacc
    16981 catccttttt cgcatcgggt cttgagtcga atctccttgt cgagatccct tcttcccgtt
    17041 ctttcaacct tctgctcaat ggcgtcccgg gcattgacga gcaagttgat gaagacctgc
    17101 tcgagccggt ttgcatccgc caggaccggg gggagatctt cggccaactc cttgaccact
    17161 cgtatcttcc gcagcctcaa ctgctggtcg aacatttcgt gggcccggct caaggcgtcg
    17221 ttgacggaaa ccggctgctt ggtcacttcc gcttttcgcc cgaaatcacg aaggtgattg
    17281 atgattttgg ccgcacgatc cacgtaggcg tcgatctcct cggccatggt ccgcaggatg
    17341 tccggagaga tgtcttcccc tttttgcacc ttcctcaaaa ggtagttccc ggccgtcttg
    17401 atcacggaca ggggttggtt cagttcgtgg gccacccctg cggacatttc tcccagggtg
    17461 gccatcttgc tggtttggat caattgctgc tcggccaaga gcctcttggt gatgtcgctt
    17521 gccaccacga gcaacgcccg ccgctccgaa tactccgtgg gcgacacgtg catgtccacg
    17581 aaaatggttt ccccccgacg agtgatgtgc cggacctggt tgaggacccg ggaggacggc
    17641 aatcttccgg cgtaactccc ctgctccgcc tcttcgaaga ggtccagaaa ggattgatgc
    17701 accatgcttt tcccgtcgaa gccgtataag gcggacgcac tctcgttgca gtcgagaatc
    17761 tccaaattct ccccatccac cacgaacacg gcattgggaa tgttgttgaa gattccgtgg
    17821 tatttccgtt ccgatttgag gatttcctgc tcgagccgct tggcttcggt gatgtccaaa
    17881 ctgatttcca tcacggcaca gacttccccg gcggccgttc gaatggggga ggttcgcaca
    17941 aaccagcggc tctcggtgcc gtcacggttg agcaccacct cttcccccac gtgattctcc
    18001 ccatcccgga aagtctggag gacggggcat tcctcgcacc gatcccggcg acccttgtag
    18061 gcccggtagc agaactcgcc cggggtcgga ttgaaccggc gatcgatctc ctggttgaac
    18121 tggatgattc ggagctcccg atcctggacg gtgatgcagc aggggacctg ttcgaaaaga
    18181 cgctggtatt cctcccgctg cttgttgagg gtctcctgct tttcggcgat ttcccgtccc
    18241 atttgctgga tggacagggc aagctggccg atctcgtcct tccgattggt cccgtcgggg
    18301 ttccgataca ggccggagcc gatcatgcgt gtggtctgga tgaggtttcg aatgggcaaa
    18361 ttcacaaaga agaagaaaaa aaccgtgatg aaggaggcaa cccccagaaa agaaaagagg
    18421 gccagagcaa tgattccctc cttgtgagaa cggatctcgt tgtccatgcg gctgagggaa
    18481 aacaccacgt ccagggcgcc caggaccttc ttcccggccg gatggtagtg gcaggaagct
    18541 tcggaacagc cgggttcgtt gtagataggg ctgatgattc ccaggagccg ctcgctttcc
    18601 ccaaccttca agacccgggt gcgttccccc agggggaccg tctcgagggg ggactcgcct
    18661 cgatggcaaa tgatgcaggc ctccgcccga atatcggtgg agttctccac ctcttcggcc
    18721 aggttggaga acttgatccg tccttccttg ttgtagatcc ggatgattcg gatgtccggc
    18781 cggcgaccga tgttgaggat gatctggttg atgtcgtccc gggcgttgag catcatggca
    18841 tagtgggcgc ccagcttgat ggtgttgccc agccggtcgg cctcctcgac catgtgaccc
    18901 atggcgttcg tctcatggta ccggatgttg gcataggccc agacggaaat gcttcccaga
    18961 agaatcaccc cgacccataa aatgagttga acggtgagac tttggcgtat gagcgagatc
    19021 ttttccgaca tccttctcct cggggatggg tacgcttggc tcccgttgtt ttcccgggca
    19081 gacgatcccg atcttcgggt gaactacggg aataatcaag aaccgggcca agacaacccg
    19141 gatgcaaacg gaacctgccg cagccgttgg gcgggcgatt ctcccggccg gggtcccgga
    19201 gggggaaccc cgtccaccgg gcgcgatttg ttccctccct tcccgggaga atccggtgga
    19261 tggtgccgtg agggggcctg tgagcgtgtt acaagggact aaatgccggc ggcggcgtcc
    19321 tcagaaacgg ccactgggaa atcggggcgg ggggaagccc gatgagccgc atcatgccag
    19381 gttgtgccgc ttcattttgc gccgcaaggt attcttgtcg atgcccagct cccgggctgt
    19441 ggccatccgg cgccattcat tgcgttcgag ggcttcccgg atggcacgct tttcgatgtc
    19501 ccggagggtc aatcccccgt agtcggggca cccctctgcc tgcccgcgca ggtaatcggg
    19561 aaggcacttg ggctggatca gcccctctcg acagaggatg aacgcgtatt cgatcacgtt
    19621 ttccagctcc cggatgtttc ccggccattc atggcgcatg aagatggcca gggcctcatg
    19681 gctcaatcca aggatgttct tgttcttgag cttgttcatc ttatcgatga aatgttcggc
    19741 caggaggggg atgtcctctt ttcggcgggc gaggttgggg agttccagct tgatgacatg
    19801 aatccggtag tagagatccc tgcgaaagga gccttcctcc accatttttg ccaggtcccg
    19861 gttggtggcg gccacgacgc gcacatccgc cttcacggat ttcgatgaac ccaggggttc
    19921 ataggaatgg gattcgagga ccctgagaag tctcacctgc agggcggggg agatatcccc
    19981 gatttcgtcc aggaggaggg ttcccccttc cgctgcggca aagcggccca cgcggctttt
    20041 cttggcatcc gtgaaagctc ccgccgtgtg tccgaaaagc tccgattcca ggagggtgtc
    20101 cgggagtgcc ccgcagttga tggccacaaa gggacccttg gctcgagggc tcagaaaatg
    20161 gatggcccgg ctgagcagtt ccttgccggt tccgctttcc ccttgcacca ggaccgtgct
    20221 ttcgctttgg gccacctcgg gaaggatgct gaaaagcctc tgcatggcgg gggacttgga
    20281 gacgatgtcc ccaaagcggt actgcttatc gatttctttt cgtaaggttt ccaccaggct
    20341 caggtctcga aaggtctcga ccccgccgat gatttccccg ccggcatcct tgaggagagc
    20401 cgtactgacg ctgatgggga tctccttgcc gtctgcgcgc aggatggcga tgggtttgct
    20461 caccaccggt ttgcgcgact ccagggtgta cttcaaaacg caggtggtct cgcagacgtt
    20521 ggctcgaaac acctcgcaac aagggcggcc gatggcctcg acggccgaga ctcccgtaat
    20581 cttttccgca gcccggttga aggaggtgat ctgccagtcg agattgatgg taaagacccc
    20641 gtccgcgata ctgtttagaa tgatctcggt ttggggagac agctcggggc accccgacct
    20701 gcctgcccca gcgcgttggg attttgtggt tggcgacttc acggaatctt gtttcctcat
    20761 ctctcgccgt tctgccgccc cccgtcgccg ggcgccttca cctcgggatt agcctcgagc
    20821 attcccgatc cccgcggagg gaccccgccg ggaccgcctc ctctggcctg ccgattccag
    20881 cgatgtcgcc ctggaacaag gataagaccc cttggggacg gggaggacga tccatccact
    20941 gcagtccctg caggattccc ggttgtgccc ggactcactg tacgaacatg cggatcagcg
    21001 gtggaaaggc aacaatccct gcattgaact atctatagca ttgttggggg catgaataaa
    21061 gccctcccgc gccctcgcgg ggcgaatgag ctctttccac cccgatccga cccttccggt
    21121 ccccctgaag gccctgtccg ggagagtggc aagggcgacg aggccggttc gcgaactcgg
    21181 tcgcgccaca ctccacgaga cgggggcacg ggactcgatt catggtcctc gattaggagg
    21241 accggacccc gggcgtcaca gggtaaaaga ggttctctag tcttctgtgg tcttggtttt
    21301 cagccgttga attcgatctc gaacgtcctt gagcgcatcc tccaggtgct gagcttgcga
    21361 cgtcaggatc tgcaaagtct cttcctgtgc gggacctgca gccgaagacg gttcgccggg
    21421 cctgttccga atcccgggac cctgccatcc gggagatccg tatcccggac ccgttggccg
    21481 ggccggtccc cttccccaaa gccggcaccg atttccaaag ccgcctccgc ctcgtcctct
    21541 gccgccccaa aacggtgtcg gcgcaggcga accacaacat actcctcgtc cccttccggt
    21601 catgggtccc atacccagcg gtccggttcc gtctcttccc ggcatggcga attcctcctt
    21661 tccgactggt tgtccttcaa acatacacgt tccagcccag cccgggacag cctcgatggg
    21721 caggtccccc cggtccggga tcctcaccat acaaaatccg cgccctttcc ccgtcatggg
    21781 accccttccc gcgggtcccg ctgcgtctcg atccggcatc ctgcttcctt tccttgggtt
    21841 tgccgcccgc ggttctcgtt tgtccggcgt cccgggaccg aaaatgtcgg ccgcggagag
    21901 cgaagtcatc ggattttcca caccgccttc gtcccttccc tctgccgtcc tacgatttgc
    21961 ccgaaaacgc ctcccgatcg aaagagggtt gaaggccgga tcccaggaag gatctcacct
    22021 ggtcccagag ttccatgacg gcggtcctcg ccggtccgtc tccatgctcg agaatcgtct
    22081 ttcccacgag ctgtgcccgg gtgaagaccg gatcgaaggg aaggcgcccc acaaattggt
    22141 gtccgtggcc ccggcaccat tgctcgatgg agtcggcgct cgggggatgc agatcggcct
    22201 tgttcacgca aaccaggacc ggaacgtgga agccccgggc aagcccggct gcccgttcca
    22261 gatcgtggtg gccggatacg gtgggttccg caacgatgag caccgccgtg gctcccccga
    22321 cggcagcgat gacgggacat ccgatgcccg gcggtccgtc ggtcaggatc atgtccaggc
    22381 ctttttcttc cgccactcgc cttgcctgct ttcgaacgag actcaccaat ttccccgaat
    22441 tctcttcggc gattcccagg cgggcatgga cgaggggacc gtgacgggta tcggaaacga
    22501 accactcccc gcacagattg ggccgcatgt cgacggcccc ttcggggcag aagtacgcac
    22561 agaccccgca tccctcgcaa agcatttcgt ccaccacaaa ttcctcggag accgcttcaa
    22621 accgacattt ctttcggcat tccccgcaac ggatgcagga ttcgggttgg atgaaggcct
    22681 tgtatccggc ccagaaaccc tccctttccc cgatacgggg agagagcagg aggtgaaggt
    22741 cggccgcatc cacatccgca tcgcagagga ccttccgcga agccagggcc gcaaaagccc
    22801 ccaggatgct ggtctttccg gtaccgccct tgccgctgat cacaaccagc tcgaccattt
    22861 tcgagtcctt tactccggga cgagaagccc ctggatgcgt gagtgcaggt gcagcatccg
    22921 ctctttccag cgggggaagc gttcgaccag gagttcccca cgagcataag cctcggccac
    22981 cgctctatcg aagggaatct ccaggagaat cggaatcccc tccctgcggg cgaaatctcg
    23041 gattcgacca tcccccacgt cgcatcggtt gacgacaatg ccggcaggaa ttcccatttt
    23101 tctcacggct cctgcagcca gttccagatc gttcaggccg aagggtgtcg gctcggttac
    23161 gagcaccgca cagtccgccc ggcgcaggga agtgacgacg ggacaagagg tgccgggagg
    23221 agcgtccacg acggtgatgc ccgtttcctc cgcctgggcc accactttcc gaatgagggg
    23281 gggcgccatg gcctcgccca cccggaggcg accctggaca agcttcaccg tggaccgctt
    23341 tcccttctcg acgactccca gcaaccgacg gctgtccagg acggccccct cgggacacac
    23401 ggcgaagcag ccgccgcacc catggcacaa ctcggggaaa gccagggcca cccgggtctc
    23461 cgggacaatg gctacggcat tgaaccggca gatttcccgg cattttccgc aataggtgca
    23521 ggcttggttg tcgatgaggg ggacttccat gtggacttct tcccgttccg tgacggctgc
    23581 tctcaggaac agattggcat tgggctcttc cacgtcgcag tccaggagct gaacctcccc
    23641 gggaagagcc atcgcaaggt tgacggcaac cgttgtcttg ccggtcccgc ctttgccact
    23701 ggcaacggca atgatcatgg ttcagggtcc tccggcttta agagaggatg tccaaaagct
    23761 ctttgttgtt tcgagtgcgg cggagcacgg aataatcgcg gttccttggg gacgatggtt
    23821 tcacgccttg ggttcgaaac ggcccttttg cggcacgatc gaagggttgt ctccggcaat
    23881 cgatccgggc gtcctcacga cccaagatga acggccgcga aatgaggggt gcgagggacc
    23941 tgagtccccc tcacccctgt ggcgcaggat gatttggtgg cccgcgcaca tggaaacctt
    24001 gcctttctca agaaagatgc cgcgtgaaca ggtcggcggg atgcctccca aaaagagcag
    24061 gatggccggg gttcccacct ctcgggtgcg gtttcgaccc gacctctcca catcttccaa
    24121 cgagatcgcc gggcgcatgt cggcctttca ggttggggtc tgctcccgga gcatgacgtg
    24181 tccgcactgg gcacaccgga cctgggtgca gggcacccct cgaagatgca cctccttgta
    24241 gccgcatttg ggacagaggc atgagccccc ggggccggtt cgtccatctc cccggcctcc
    24301 ctgccgaccc tggcctcttc cggttcctcc ccggccttga ccgcgttgat cctggagtct
    24361 tccaggtgcc atggattctc tcctttccga aacggcccgc tatttcttca ccatccagtc
    24421 gtgaacggcg gcatgaaggg tctccgccgc cagttgggcg cagtgggctt cgttctcagg
    24481 gacctctttg aacatactga ggacggtatc ccccccgatc tccaccgctt cttccacact
    24541 cttcccccgg gcgagctgtg ttgcgaccca gccgcagagc acactgaacc agcatccgtc
    24601 cgagaagaag cttgcatcga tgatcttatc cccgtccagc ttgaggaaga cctccatggt
    24661 ttccccgcag cttcccgtga tcttccccgc acaagaggcg tcttccattc ttttcatgat
    24721 tcccagttga tcccggcttc ccgcggctgc tttctcaggc tgttgccccg ggttctttcc
    24781 gaccctccgg tagatggctt ccatcatgct cttggtcgaa ttgctcatgg gactcacacc
    24841 tcgctagtgg tcgcacacat tggcccccgt tgtgagggag ccgttcaagt actggtggat
    24901 gagttcctcg ggagcaagat tgggtgcccc ggtgatcact cggatgttct gttcctcgaa
    24961 gaggtccaac gcccgcttgc ccatgcctcc cgcaaggatc aggttcactc ccaggccccc
    25021 gagccagcga ggcaaaaccc cgggttcgtg gggaggggga gtgagaagct ctttcccttc
    25081 gatgcgaccc tccttgacat ccaagagcgc aaactgttca cagtgtccaa agtggttggt
    25141 gagtctaccg tcggccaaag ggacggccac tttaaagtgc tcggggagat caggtggagc
    25201 gaccttttcg ggtttgggat cgctctggaa gctcgggttg ccgcagagcc cgaccacctc
    25261 gagcaccaat tcccccacgg ccttgacgaa cggacttccg tccgggctgt cgagaaggga
    25321 ccggccgacg tctcccgctt ccaccaccct ggggtcgaag ggcaggcttc ccaggagacg
    25381 aaccttcatg cgcgccgcgg tcctggctcc tccgcccttg cccagcagag gcaattccgc
    25441 attgcagtgg gggcatacat acccgctcat gttttcgacc agccccagga tcggcatctc
    25501 gacttttcga cagaagttga tggatttcct cacgtcggcc agggcaactt cctggggagt
    25561 cgtcacgatg acggccttgg cgtcgggcat ggtttgggcc acgctcaaag gttcgtcccc
    25621 ggtgccggga ggcgaatcga tgatcagata gtccagctct ccccaatcga cgtcggatat
    25681 gaactgctgg atgactccgt gtttcacggg accccgccag atgacggccg aatccggatc
    25741 atcgagcagg cattgaatgg agaccacctg cagcttgtcc ccataggtat ggggaaccat
    25801 ccgtttgccg tcgttcacac gaaacatgcc ttccactccc agcatgcggg ggatactggg
    25861 accatgcagg tccacatcga gcaagcccac ccgatagccc agcctggaca gcccgatgga
    25921 aaggtacgtg gccacactgc ttttccccac accccccttg ccgctcatca ccagcagctt
    25981 gtgtcctatg tgccgaagtt gctcctgcag ttgtttctct tccgaggatt tgggttcatg
    26041 gccgcactgg ccgctatgac aagaactcat ggttcaatct cctttggaag taataggtcc
    26101 tacgaacgtc attccacatg ggcgtcggaa accagtcagg gcaaggaatg tgccacttgg
    26161 gttcaaacga ataatccttt ggaatgacag gcaaaccatg gagacctgga gaaaaggtcc
    26221 tcccaaaggg aaccgtttgc accctgcggg ttcctccgaa gaagccatcg tgagcccctg
    26281 ggaaaaagga acaaagaagg agaagagagg gctcatccgc ggaccatgga ccggccgcac
    26341 gcgggacagg ggacttgccc acaggggatg ccgttccgat gaggaatccg ggctccgcat
    26401 tcgggacaca ggcaggagcc tccggggcct gcggcaatct ttcctttggc acccgtttcc
    26461 agaccccggt tccgcccgcc ctccccgcct tgcctccccc ggctctttct cctttcgagg
    26521 ctgggacagg attccgccgc agcgtccgcg gcacagaccc ctttgcatcc ttcccgataa
    26581 ttgcgcggtc ccttgcagcc gggcagtcgg aaacgagggt ggtccagctt ctgtcccagg
    26641 taagcgcgca gcacctctcg gacttcgcct gcgaccccgt gaatgatccg gattcccatc
    26701 ctttgcccgt agtgaagaag atccgggctc aagaccccgc agatgagtgt tcggactcct
    26761 ttgacgcgaa gaattctcat tcgatcgaag gtggttgcat gggtcaagaa cagttcttgc
    26821 tccggagtct cgctgccgga aacctcggga tagagatgaa tggtggagca ccaatccagc
    26881 acaggtgcca ctcgcccccg aaataccgga atggcgatca aaaagattcc ttcccctgca
    26941 atttcggagg cctctttatt gaaaccgaaa gggattctct gggccatggt cccatccttg
    27001 cccgtgggga ctgaagtgct ctttcgaaaa ccgtgctgca agacaccctc tttcaaaaac
    27061 cacaccagga aagggcaaga aacgcagttc ccgttccacc cggccttgtg gccataaaac
    27121 tcggtccccg ggatccggga gcgcatttgg ggagaaccgt gaccgacgca ttctgcaccc
    27181 aactccggga gtttcagggt gcgcaatgac ttgtcttttt cccacgggga aagttgatcc
    27241 tggaaaggtg ggcgtcgtgt ctgcctttcc gggagttacc atggggtggg gctcttggca
    27301 caatcgttgt accgcaacgg tgatgcggca aggtcgagac aagacggaac ggagggaaac
    27361 gcacggagtc atcaaaccaa aggagtggaa ccatgaaaat tgccatcagt tccagtgccg
    27421 gaaacctgga cgcagaagtg gacccccggt ttgggcgggc cgcctgcttt ctcttggtgg
    27481 attcggaaac catggacttt gtggtactcg agaatcaaca aaactaccag gccgcccaag
    27541 gggcgggaat ccaggcagcc accctggttg cccgacacaa gccggcggcc gtcctcacgg
    27601 gccattgcgg tcccaaagcc tttgaagtct ttcgatgggc cggaattccg gtcgtcgtgg
    27661 gcgtgagcgg gcgagtgcgg gacgctgtgg acaaattcct gggggatgga tttaccccgg
    27721 cttcgagtgc ggacgtggcc ggccactggg ggtgaggtcg agggaggccg acggcttcgg
    27781 ggccgggacg gatcagtgca tgagtcgctg cacctggccg ggaaaccgct ggtagacttc
    27841 gtgggccaaa gcgatgaggg attcttccag gagctggtaa cccggcaggc acaagggttc
    27901 ggggacggcc attttttcaa aggagacggg acaaaggcac aggcttcgct ccactgccgc
    27961 caattcgccg ggggtgaaat tttcccccag gaaatgcttc cgttccagac cctggatatc
    28021 caggcttttt agaaagctca acagggacag gaggtcgaag ttcttctgaa agccctggat
    28081 gttgcagttc accgcccggc attccaggga aaggtcttcg tactccttgc ggtaggactc
    28141 catccagtac tgaaatcgct gaaaggcctc cagaaacagc tttcgatacc gggccttcag
    28201 ggtgaatccc cgaacgcgga tggtccgaac tgtccgggga aaatgactgt tgaaacaggc
    28261 gttccagtaa gaagacgggg agatgcaaag cagcttcctg agccgctcga gcatttcggg
    28321 gttgatgagc aggagtccca cgcggcacag gcgtttcccc gtttccacgg cgaggcagcg
    28381 gaccttttcg gcccgctgtt cgagctcttc catctggagg tggaccagtt ttcgctcgtt
    28441 gagatagttc tcgatgactt cttctttgac ctgacgggta aggctcgcaa caagatcatc
    28501 ttccatgagt caccctcaac cggagccccc tcttgagccc atgcgcttag cgacgttgaa
    28561 gaagataggt ctggggattg agaaggaccg caggtcgctg cgggaggtac tggccctcgt
    28621 gttttagaac tttgacctga atgacgtcgg gaacgggctc caggaggaga atggcctcga
    28681 agagcccatc catttcatga cacggatagg gaatcccgcg ggcattggtg gttgacatgt
    28741 gccgatgcat gcgggcggac atccacatgg agaccccgtg tttcccggca agttccttga
    28801 gttcctcgac ggaagtgcgg gaaatccggt cgaaatccag accgtccagc accaccattg
    28861 caggctgaaa gttggcctgg tccctgaggt tccgcacgct ctgatccagt ttccccgggc
    28921 tgaaggtatg gtgcaggtac gcatggatga agcgcagggg ctcgatttgc tgttgaattc
    28981 ggggcacgtc ccgatggtgt ccgggaccca ggatatttcg cagcagttcc tggtaccaga
    29041 tcttgatctt ttccggggtt tcgtcgatgc agacatggag aaccggaaag ccgtgnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntcaca atttcctcgg caattccccg
    29221 gggaacgggg ctatagcggg cgaattccat cgtgaattcg gcctttccct gggtcgtgga
    29281 acgaagaacc gtcgagtacc cgaacatttc cgccagaggg acttccgctt cgatggtgga
    29341 gaaggcggcg tcctcggagc tgctcacaat catccccctg cgttggttca acgtacccag
    29401 caccatgcct tggaactccg aaggggtttc caccaccacc cgcatgaggg gttccatgag
    29461 aatgggaccg gccttggcat aggcttcccg aaaggcgccg atggccgcct gctgaaaggc
    29521 caaatccgaa gaatccaccg aatgggaggc gccatcgttg atgaccacgc gagtccccac
    29581 aatggggtag gccgccatgg accccttgga caggcaagcc tgaaaaccct tttcgcacga
    29641 agggatgaac tcggtgggga tggcccctcc ggtgatctgg ttcacgaatt ggaagtcccc
    29701 gtccggataa ggctcgatat aaccggcgat ccttccgtac tggcccgcgc ccccggtctg
    29761 tttcttatga gtgtagttga agtcggacct tcgggtgatg gtttcccggt aggccacttt
    29821 gggcattccc gtttccacgt cggactggta ctcccgcttc atacgttcca cgtagacatc
    29881 caggtgcagc tctcccattc cgcagatgat ggtttccccg gtttcctcgt ccatgatgat
    29941 ccgaaaggtt ggatcctcct ttgtgaaccg ggcgagagcc ttgctcatgt ttatttccgc
    30001 ttttttgtcc ctgggtttca aggccaggga aatcacgggg gtaggcacgt gcatggacgt
    30061 catggtgtag tggacgcggc cgtcggtgaa ggtgtctccc gaaacgcaat cgacgccgaa
    30121 aagggccacg atctggccgg cgcccgccga ttcgatttcc tccatttgat ccgcgtgcat
    30181 ccgaatgagg cgtcccacct tggtcttctt accggagcgc acgttgacga tggtgtctcc
    30241 ttttcggagg gtgccctggt aaaggcgcac ataggtgagt tgaccgtaac gcccgacttc
    30301 gagcttgaag gccaacatca ccaggggcag gtccggggcg ttgaacaggt ccaccttggc
    30361 ttcgtcccga tccaggtcca gggcttcgtt gctcttgtcc atgggactgg gcagatagcg
    30421 gcatacggcg tccaggagcg gttggactcc cttgttctta taggcggacc ccatgaggac
    30481 aggagtgatc tgcagggaaa gggtccccgc gcgaacggct gcgtgaatca agtcctccgt
    30541 cacgcgctct tccaggatcg cttcggtgag ctcgtcggaa tagagggaaa ccgcatccag
    30601 catttcttcg cggtgcttca tcgccagatc catgagctcg gcggggattt ccgtccgggt
    30661 gaccacggag ccgtgatccc cctcgaacct cacggcttgc atggcaatga ggtcgattac
    30721 cccttcatga tccccctcca accctacggg gacctgcaaa ggaacggcgt tgtgattgag
    30781 tttttccttg agctgacgga ccacccggaa gggatcggca cccgttcggt cgcacttgtt
    30841 gatgaacgcg agcctgggga ccctgtaacg caccatctgg cgatccacgg tcacggactg
    30901 ggactggacc cctcctacgg agcagaggac taggatcgcg ccgtccagga cccgcagggc
    30961 gcgctcgacc tcgatggtga aatccacgtg gccgggagtg tcgatgatgt ttatgggatg
    31021 gtccgcccat gtgcaatggg tggcagcgga ttgaatggtg atgcctcgct ccttctcgag
    31081 ctccatgaag tccatggtgg cccccactcc gtccttgccc ttgacgtcgt ggatcgcgtg
    31141 gatcctctgg gtatagtaca ggatgcgctc cgtaagggtc gttttccccg aatcgatatg
    31201 ggcgctgatc ccgatgttcc gaattttgga aagatcctca accataagga actctgccgc
    31261 actcctttcc ataagaactt ctcgccgacc aggacggtcg cagaataaaa caaaaacggg
    31321 cactcactgc ctgtgcccgt tgggagctcg gttatctaac agaatttaga tcggaagaaa
    31381 agacccaatc aacgggtatt cccccggtac cctcggctca tcgcctggtc gccacctgct
    31441 tggaagtctt cgccgtggtt acggtgttcc ctttggcgtt tccaggggcg gcgggtgcag
    31501 gcttcttcac ggaggcggac accttttcct tggaagagga gccggccttg ctcttggcat
    31561 tgggggatgc ggaagtcttc gctgccgtgg cggaagaagc cgctttggat tcaggggccg
    31621 tttttcccac ggatttcccc gatgcctggg ccccgttctt ggccgggttc acgccctttg
    31681 tcgcggcggc tttcttcccc tggggcgcat ccttaccttg aaccgctacc ttcttggaag
    31741 ctccgttctt gggctcggcg accggcttgg cgggaaccgc cttgaccttg gaaaggttct
    31801 ttttgggatc ggctttgcct acggcagctt tcttgtcggg ggatttggag acggctttgg
    31861 gtcctcgatc gctcattcgg ccaggttccg acgtcgggcc catgcccttg gacatcgttg
    31921 ccccctgggc cagccgtatt ttttggcccg gtttcagtgg ggcgttcctc ttcattgcgt
    31981 tcagttggca caaggcgtcc agggggatcc cgctcttcct ggaaatcttg gccaaagtat
    32041 cccccttgac ggaggtgtag gtgaccgtgg tggtgccttt tcctctcgac gcgtccgtca
    32101 tgacggtctg tcgagtggaa atgtcgtgcc gcccgaaccc ggagctcgtc gaagaggcgg
    32161 cggtcacgga cccggaagaa cggccggccg tcactttgag tttttgaccg ggcttgagtt
    32221 gggggacctt cttgaagccg ttcatttgga gcagcgccct cagctccatc ttgttcttcc
    32281 tggcgatgga ggccggagtg tctccccgct gaacggtgta ggaagcagtg gaaggaggct
    32341 cgaccctttt ctcccgaggc acgtgcctca ccaccgcagc gggggcctcc tccacggcat
    32401 cggagaccga atccggagga ggcgtcgggg tctcgggacg cacggggcgc aaagcggcca
    32461 gatgcacccc gggggcttca aagttcggcg acaaggcctg agccaggtga accggctcca
    32521 gctcaaaccc ccttttgtaa aaaaccaggc aactacccgg agaaatggga gagttcccgc
    32581 tcagctggtt ccatcgacac aggtcccgat actgcacgcc gaagctccgg gccagggatt
    32641 cgagactctt ttcgttgcct ttgacgtagt agtttacctt cttggggagg aatgcctgct
    32701 gggccatttc gatgaccgtc cgcttctgtt cgtccattcc tgccacatga gcggccagag
    32761 tggaccatgg ggtgtagaat cggacgtgaa tgtggtcgtg atggttggcc acatgattga
    32821 tgacggaacg tccgaagccc cccccgaaaa tgcgctccag gtatgcggag tccgcacccc
    32881 gggaggcggc gtagtcatgc aaaagccgct ggattcttcg atcgagaaag atgtactgca
    32941 cacgctggga acgaagaaga ccctcaacga ggcaccacgt cttggggaca tccagattgt
    33001 cttcgttcat ggagacaaat gtgtccgcag ctcgattccc cttgaggtac atgcccaggt
    33061 ccacatcccg cccgttctgg tgggaacggt gatggttcaa ccacccgccc ccctggacgg
    33121 aaaagtcccc cacgaaaagg tcgcaggtgc cgggatgctg ttcccgcacc cgttccaccg
    33181 cgtccagcac ggcacccacc acttcgggag tggtgtagga gcgctcctgg tcccgcaggg
    33241 tgtacccttg gaactggttg gggaagggaa ctccgttgac cagccgccca tggtacggct
    33301 ggcccaccga ggcggtgatg ccgagaacgt cgttggggcc caacataact gccggaatac
    33361 acgttaaaat aaggcacaat ggagttagcc tcattcttag gcaggagtca aaacgcctca
    33421 ttcaatcccc ctcctattaa tgactcatca acaactaaga ttattggcct gtacgatagg
    33481 gagtccaaca gagatgccct tcactaccga aaaaaaggat gggtgtcaaa ccttttttcg
    33541 cgaatggacc atacaatttt ttatagaacc ggataactaa ttgaatatag tgtataataa
    33601 acaaatacat cctggtccgg cctcagggtg cagatatccc gcacgaggat aattctttgt
    33661 aaaccaagaa atctgctatt gaatacagcg ctttgataac actggaattg tggtgtcgaa
    33721 ggaggatggt atgcgggatc gggccccaac ggcctacgac cgctgagtcc cctcctccgc
    33781 gaaccatcca tacaggtcgc cgcagaaaac cacctggccg ttgccgtccg atgaggcggg
    33841 aacgtactcg atgataatgg ggaccggctg gttgaatttc acgaaagtct ggcgtgtgta
    33901 gctttcgaga aaggattccg tttttcccac ggccgaattc ccatcgtcgg ccaggaggag
    33961 ttgagcgagt tctcgggcct tttccacccg gatgcatccg tgggaaaaat cccgcttggc
    34021 tcgctgaaaa aggtgtctct tgggcgtatc gtgaaggaag atggcgtagg cgttggagaa
    34081 ctcgaacttc acccgcccca aggggttccc gggtcccggg agttgaaaga ggcgaggctc
    34141 tccccaggga taattggacg acatcttcac gtagccgtgt ttgtcgaggt agttggggtc
    34201 cgctgcgatc tcatcggcca attctcgacg gatgcgatcg gatacatacc atcgggggtt
    34261 gaacaccacc tggtcgatga cactgctgat ggagggggtt tgattttctc ccatccagcg
    34321 cccctggacc ttcacctttt tccctgcggc cttgcccaca atgacccggt gttccgcctg
    34381 gatcttcccg tcccggtagt attcgagcat gaattggggg atgttgatgc gaacgtatcg
    34441 atcgtaaggc cgggcccgac tctgccgaag ctgcttgaga ctctcctgga gcaaccggac
    34501 cttcttattg taagggacat tcaaccaatc cttggttctt tgaccgaccg ccccgtccgc
    34561 gtcaatcaag tggtatcgct gaaatttgcg gacggcatcg gccgtggcat ggtcgaaatg
    34621 ccccgagatt ttccccgaat agaacccctc ctgggcaagc cttttctgaa gattcgacac
    34681 ctcctggccc gaatctccct ggcgcagggt ctttccggaa tggaagcggt gccatggctc
    34741 ctgggccgcg aggctgcggt acttggtcag ggcttttcgc aaggtggcgt aatgaggcga
    34801 attgggaaca acggcctcga gaaactcggc catggacatc ttccccaaga ggaccttctc
    34861 tcgaagctcg ggagaaaagg gattcatttc ctgggcaaag cggatcaggc aggcccccag
    34921 cttcacatcc gtctcggagg cgacttttag gagctgcaaa taacggggat ctctttccac
    34981 atcgctcagc cggtccgttt ccccgtttgc aggggcgggt tgggaggcaa ccgaggcttg
    35041 ggagggcggc ggggatgggg aagcctcttg tccggctccg ggaggattcg ccccagtccg
    35101 atcctggttg gccgccggag tgggctggag ggccgcccgg agctcttcga cttccttgag
    35161 tccctttttc agggaatcca ggggaaagct tgcggggttg acggcgtcgc tttccaggtc
    35221 ctccagtctc ttgagaaaca aagcggttcc gggggcgagt tggaatcgga agtcaaaaaa
    35281 caagggtttc caatcgtttt ggccgtagac atccaccagg gttccggggc cggagtcgct
    35341 cagaagctcc gcaggcacgg cttcccagag ggcggctcgc cagcgggaag cggtgtcttc
    35401 ggccacgcgg gattggcctt ggggcgatga aaggggctcc tgggaaggac gggtctcctg
    35461 cgccgccagg tcgggaggat tgagaaagca tggaagaaga atcaatgaaa ccaaaacgat
    35521 gccgaggatc ccaaaccctg tcgaggttct ttccgccata attccttccg ccgccttttg
    35581 aggtcgcatc agacgcaaca gttactcatc cgcctttgta ttgattctct cgtagaggca
    35641 aatctggtcc aaaagcgaga gctttcggcc cgggttcttt gcccctatat ttcccgaacc
    35701 ccggcaagaa tggcaacggg cctcggaaca caactggcaa aaatggcaat cggggcaggg
    35761 gtgcttcttg gcgccttccc ccctcttctt cggctcatag acgcgcttta gccccgccag
    35821 ggggacaaag cagctttcgt tggactctgg gctcgggtcc atgattctcc cccgaattga
    35881 gcgattcgct gttggtcgtc agcactttgg tattgtatct caacattatc ggacgtcccc
    35941 tgccggggga agccaaagca gctaaaccac gggagattgc caaactaacg gctgaacgcc
    36001 caatctggat ccctctctcc cgaggtccgg ggacacgccc gtacaatccc ggtggatttt
    36061 cccggggact cccaaatcct catcggcccc gggcaaccgg cttacggaat ccttgaacgg
    36121 cacgtattat aagatccacc cgctttgaag ccaagtacat aaaagggctt ttcgatacgg
    36181 tacatttgtg ctaatggcag ggccccctag aaggtccaag ggtgaagggg tttttccttg
    36241 gggcctccgg ggggcgaacg gcggtttctt ccggaaaacg gcagaggatt ctccggaggg
    36301 gggttggatg gactcccctt cacggcaggc agcccctgct tccgggcaac acgggacccg
    36361 agacggcctt ccgacggtcg cggcctttgg gaaggaggcg attctgagca aggagcacca
    36421 tcgctggatg ggagtggcct tggaggaggc ccggtcggcc tacggggagt ccgaggtccc
    36481 ggtgggggcc gtcttggtgt caaaggacgg cgaacttctg gcccgggcac acaatgcacc
    36541 cttggggctc tgcgacccca gcgctcatgc cgagctcctg gtccttcggc aaggagctcg
    36601 ggtgctgggg aactaccgac tgccgggaac cactctgtat gtgaccctgg agccttgtgt
    36661 gatgtgttat ggagccctgc tccacgctcg agtgcggacc ttggtcttcg gggcgtacga
    36721 ttcgaaatcc ggggcgttgg gaagcgctgt ggacttgaca accgtatcgg ttttcaatca
    36781 ttatatcgag gttgtgccgg gcgtaagggc ggccgaaagc gccgagctgc tccggcggtt
    36841 ttttcaggaa cggcgctcag agagggaaca ggcttagggg agaggtaccg aagtggccgt
    36901 aacggggtcg actcgaaatc gacttgtcct gtgaagagca gggcacgtgg gttcgaatcc
    36961 caccctctcc gccatagaac cacaacctgc ccgcggaagc ggtcgcccgt tattttgcgg
    37021 agagatgtcc gagctggctg aaggagcacg actggaaatc gtgtgtacgc ccaaaaggtg
    37081 taccgagggt tcgaatccct ctctctccgc cacttttccc ctgcatccca ggtgttcatc
    37141 cagctatctc cctgtcgagg attttcggcg gacccatgcc ttacttagtc ttagcacgaa
    37201 agtggcgccc tcagaccttc gatgaggtca tcggccaacc tcacgtaacc caaatccttc
    37261 gcaattccgt cgaatcccag cgcattgccc atgcttacct gttcaccgga tcccggggag
    37321 tcggcaagac ctccgttgcc cgaatcctcg ccagatgcct caactgcgaa aaaggaattt
    37381 cctcccttcc gtgcaatgaa tgctccaatt gtcaggagat cacccaaggc aattccatgg
    37441 acgtcatcga gatcgacggg gcctccaacc gcggcatcga caacattcga gagctccggg
    37501 agacggttcg gtatcgcccg ggcaagagcc ggtttaaagt ttatatcatt gatgaagttc
    37561 atatgctcac caccgaggcc ttcaacgccc tcctcaagac cttggaggaa cctccggagc
    37621 atgtgctttt cctcatggcc accacggaac ctcacaaaat cccagcaacg attctcagcc
    37681 ggtgccagag attcgacttt cgtcgaattc ccatggactt gaccgtgcag cacctgaaga
    37741 agattgtcgg ccgggagcac acccggctgt ccgactccgt ctattacgcc gttgcacggg
    37801 aagccgacgg cagcatgcgc gacgctcaga gcctgttgga acagctcctg gcattcagcg
    37861 gcgaggatct gaccgatgag gagatcctcg accttctggg agtggtggac cgcaggagcg
    37921 ttcaccgact gggacgagcc atcctggaag gggatctgcc cacatgcctc cgggtagccg
    37981 cagacctgta tcgcaggggg atcgacagca ggcgggtatg ccagcagttg tgcgaccatt
    38041 tccgcaatct gctctttttt tccctggcgg aaaccgaagg atcggtgcag ccggatcttc
    38101 ccgaagacga aaagggcatc ctgcgggaag acgtccgcaa gaccagcacg gagtccctct
    38161 atctctatat ccagatgcta ctcaagggag aggaggaaat ccggagatcc acccttccca
    38221 agatggcctt ggagatgctt ctcgtgaggc tggcgcaact gcctcgactc tcttccctgg
    38281 aacagctgat gggaagaatc gcacagttgg aacaggcgct cgagggcagg gactccgagc
    38341 cgtccgaacc ccgtcgggct tccgggtgca cgccctccgt ccctcccgtt gcgccgccgg
    38401 cggtttcctc ggaaccattg gcacccttgg agatctcgat gcctcccggg ggggtcgatt
    38461 ccccggctcc ccccgccccc caacgggcag tgagggccga agaagcggcc caaagatggt
    38521 cttctttcct cgaatggatt caggaccgtg acccggtttt gggggcaaag cttcagaaca
    38581 gccgggtcct gcccaacccg gacgggaccc tgacgttgga agtcctcgag ctcttcaagg
    38641 accgggtttc cgaacctcag accctggcaa atctctccga agcaaccttg actttctttt
    38701 caaacccgtt ccaatggaag gtggaggtcc ggcctacccc ttcggcggat tctcccgatc
    38761 ggcccaggag tcgaaagacg cgcatcaatc acacccggct ggtcatggag aaccccgtct
    38821 tccaggatgc cctggaaatc ttgggaggcg aactcctgga catcaaaccc ctgcgaacgg
    38881 gttccgggaa acggaccggg gtcgatcccg gctcgggttc cccggacggc gactttttgg
    38941 gaaacgattc cccctaaacg gatggggagg agactcgatg gaggcgacac aatggtgaaa
    39001 gggttcaatc ccatgcaaca agtgaaggca ttgcaggaga agatggcaag aatgcaggag
    39061 gaaatggccc aaaagaccgt agagtcctcc gccggagggg gcatggtcac ggtgagggtc
    39121 aacggccgcc aggagttgct cgatctgaag atcgatcccc aggtggtgga tccccacgat
    39181 attgaaatgc tccaggacct catcgtcgcc gccgtgaacg atgccctccg caagtcccag
    39241 gagatggtgg cgggagaggt gggcaagctg gccggaggct tgagcatccc cggtctcaaa
    39301 atcccgggac tcttctgaat gctttttcgc ggctgtcgcg acttttccgt gttcgtcttt
    39361 cggccctacg ggagcccctc ccgactgaaa agaccttccc cagaaaggca accattgccc
    39421 tcatgaatgc ctatcctccc attctacgaa agctcatcgg cattttgagc aaactccccg
    39481 gtttggggga gaaaagcgca acgcgcatcg ccatgcacct gcttcaggcc tctccgggag
    39541 aggccgaatc cctggcggaa accatccagg agatgaagaa acgaatccgg acctgccccc
    39601 gatgctttct cgtgacggat tcggaactgt gcggcatctg cgccgatccc gccaggaata
    39661 cgggggagat ctgcgtggtc gaaaccatgg cggaccttct ggccatcgag gggtcggggt
    39721 cttataaggg aagatatcat gttctcgagg gagcgctggc cccactggat gccgtaggtc
    39781 ctgagaacct ccgtgtggcg gagcttctga aacgccttcc ggaggaaaac attcgggaag
    39841 tgatcttggc caccaatccc agcaacgaag gagaagccac ggcccactac ctgtcgaggc
    39901 tgatcaagtc ccacaaggtg cgggtctcgc gcattgcatt cggcattccc atggggggag
    39961 acctgaagta cacggatcgc gtgaccctgg agcgatccct caagggacga caggccttcg
    40021 aataaccgcc gcccccaacg ccccatcccc cccgttcatg gaaatcttgc gcccctcccc
    40081 ggccggtctc cgacatccat cccgggaccc gggggcgcgg gctccgaatg ctttgggacc
    40141 tcctccctcg gaatgctgac ggatccgccg ggagtccggg agctcgatca caatgcagac
    40201 atccggtttc ccttttttcc ctccgtattg cgcaaagaat cttgaaagat cttgtagacc
    40261 ccctcaggcc ggtaatcgtc ataggagtca acaggtcgag gtctgaaagc gacggcagtg
    40321 aaatgagaaa tcggaggtaa ccccatgaac cgggcaagaa tcaaatacac ggcaagtgcc
    40381 atggtctttc tggccgtgct cgccttcgtc gtccccagct atgcggctcg gcagcacaat
    40441 gccggagggg gcgggcggca tcacatctcg tctaatcgcc ccatgaactc caaccgcgcc
    40501 atcaatcgag gacctgtgaa ccggggctac cggaaccagg tccatgccaa ccggtaccga
    40561 ggtcaaatgg gtgacttccg tttgaggaat gccgcacgcg ccgcggggcc cagcaataaa
    40621 cagtactccc gacgcgctta cgacaacaag cgccactgga acggccgcca ttacgatcgc
    40681 tatcgctacg gctacaacgg aaggtattgg gacggccgcc acggatacaa ccacggccga
    40741 tactggaacg ggcgttatgg gtacaaccat gggcgctact gggacggtcg tcaccaccat
    40801 cattattgga gaggaaaccg ctattactac tacggcggct ggggaggacc ctactggggc
    40861 ggctactatg ggtggggtga cggatggcct tactactact ggtcctccta ctattactac
    40921 ccgttcccct tcttctactt cccggggttc tccttcatgt tctacctgta gggtggataa
    40981 cggatcgcga tgggcttttg tgagccctta ggaggagcat ccagtgatca aaaaggtgac
    41041 acttctcggc ctcggcttcg ggttgatcgc cgcaggaatc gcaggcatca gcatgtcggt
    41101 ggtggaaaaa aagggcaagg acgccctcaa acaaatggag aagctgcagc aggaaaatca
    41161 gtccgccgcc gccgaagaat ggaagaagat cgaagaaaag cggcaggagg tggaaaagct
    41221 tgctgccggc gtcgaggccg agaaacggcg ggttcgagag gagcagcggc gggctcagga
    41281 agagcttcgg gccagggagg cgaatctggc gagaaagaaa ctcgagatgc ccggaaccaa
    41341 ggctgcttcc tcccaaaccg ccggtgagaa aggcaagaag ggaaaagcga caaaaacgac
    41401 ccttgcctca aaagactccg gaaagaagca agggactgcc gctgcgagcg gccgctcccg
    41461 cagtgtcaaa gcctccaagg cccgtgacga ggacggcttc cggtggatca gccggaaagc
    41521 cggggccgaa gcagcccgaa tctctcgccc ggtgaaatac cacaacctaa gaacccggga
    41581 actggtactg gcggaacccc tggactacgg tccccattcc gtgcgagtgc ggattcgagt
    41641 ctggaaagat cagcggctca ttaaggatac aaccatcacc atctccgaag atgccctgcg
    41701 gggtcccgga cctacccggc acggcagggg aagagatttc gcgtaaccga tccccgaagc
    41761 cccggatgaa acgggggtcc tagaggagtg tgtcgtgccg ttatcagcca tgatattcct
    41821 atgcagcttc gccattctgg tgggagcggt cctggtggcg tacggtctag cccaggagtt
    41881 tcggacaaga gagtccgtcg gcgcagagaa gtgcccgcct tttgccgggg aaggcaaggt
    41941 ccccatcgac gagcctctgg tgctggattt caaggaagaa ttcagcgtga aagagcctgc
    42001 agacgcggaa ccttcccaga cacccggttg gagtgccagg aagaacaaac gtacgctggg
    42061 agaaatcgca agcgggtttt ccgcatcggt gcttggtggg ctgagaaagg tcctgcgctc
    42121 cccccctcct gagcccggga ccgcccccaa gaccgatctg gaccatcctt cccccacggg
    42181 cacggtcatc ggcgccaaga cccttccggg ttccccggaa attgcccgtc gagaaggata
    42241 acgccggagg ggtcgggaag ctcatccgtc tgcccccggt cccgctgcat tttccccttc
    42301 cccgacccca ggccgcctcg gtccgctgtg gctgcaagcc ctgcggcttc ctccttctcc
    42361 taccaagaag aatttgatct ccccggcgca ccatccctcc cccgataccc cgggcaagaa
    42421 tcccctgccc ggggtatggt gcaatctccc acctcacaaa acggccggca cacgcctggg
    42481 gcattgcttc ccaacgtcaa gcctccatgg agcggccggt caagcctgca ctgaatgcga
    42541 gcatggtttc ggaatccgca tcggggggga gttgacaagg ggatttcgtg tctatactct
    42601 actagtccta taggatatat atccgggctg atcatgcccg cgggttcccg cggaatcggg
    42661 tccgctcaaa atcccatgag gagaagcacc aagcctatga gagatgccgc catccacgag
    42721 cagtgcaaga tcgaggtgga cgttcgccgc caatatcgag aagagatccc gggggttgtg
    42781 gaggctctgg tgcaatcgtg ccgagtggag acatgtttcg atcatgtggg gccggaaccc
    42841 attccttccc gggagaccgt catcgacatc attcacaggg tgcgacggat tctgtatccc
    42901 ggctatttca cccgggagcg cttggatggg ttcaacctcg agtactattt gggccaggaa
    42961 gcaaccgccc tctttgaggg tctttcggag cagatcaccc gggccctccg ccatgactgc
    43021 atccgccaca atctgccttg tgtggaatgt gaggcgttgg gacaacaggc ggccgttggt
    43081 ttcatgggga ggctgccgga gctgcgaaac atcctcgcca ccgatgtgcg ggcggctatg
    43141 gagggggacc ctgcggccgg gagctacgac gaggtgattt tcagctatcc cggactcttg
    43201 gccgtgaccg tttatcgtgt cgcccatgag cttctccggc tgaagattcc cctaataccc
    43261 agaatcatga ccgagtatgc ccacagccac acgggcatcg atattcatcc cggggcgcgg
    43321 atcggtgaaa gtttctttat cgaccacggc accggagtcg tcattggaga gaccacggaa
    43381 atcggggacc gggtcagact ctatcagggc gtaaccctgg gcgctctctc cctccccaag
    43441 aatgccgtgg agtctctccg agaccaaaaa cggcacccta ccatcgagga cgacgtcatc
    43501 atctattccg gcgcaacgat cctggggggg gacacggtga tcggagcgcg atcggtcatt
    43561 ggaggaaacg tgtggattac ggaatccgtt ccgcccgaca ccaaggtgtt tctcaaacag
    43621 ccggaactgg tctacaaggg tgcgtgatgc cgctgtggag gggacatggg taggaggatc
    43681 cgcttgaggc ttctcgatcc tttttcaggg agtgaatggt gggtcgaatc tttgaggacg
    43741 tttccaagac cgtggggaac actcccctgg tgaagttgaa tcgcatcgcc cgggaagcgg
    43801 atgccgtgat cctggcgaaa atagaatcct tcaacccgtt atccagcgtg aaggaccgaa
    43861 tcggcgtttc catgatcgaa gatgccgaag cccggggcct gctccatagc gattcggtcc
    43921 tcatcgaacc gacgagcggg aacacaggaa tagccctggc gtttgtggct gcggccagag
    43981 gctacagatt gatcctcacc atgcccgagt ccatgagcgt ggaaaggcgt caactcctcg
    44041 ccgtcctggg agcggaactg gtgttgaccc ctgcggccga ggggatgacc ggagcggtga
    44101 acagggccga aaagcttttg ctggagattc cccattcggt catgttgcag cagttcacca
    44161 atccggccaa cccggaaatt caccgccgca cgaccgccga ggaaatctgg aacgacaccg
    44221 acggcaaggt ggacattctt gtggcgggag tcggtacggg agggaccatt acgggggtgg
    44281 ccgaggtgat caaagaaaga aagaaggact ttcgcgccgt tgccgtagag cccgaatcct
    44341 cccccgtctt gtccggaggc cggccgggac ctcacaagat ccagggaatc ggtgcgggct
    44401 ttgtgcccgg ggtgctcaat cgaggaatca ttgacgagat cattcgggtc aaggatgagg
    44461 atgcggggag aatcgcgcgc cgactggcga gattggaggg gcttttggtg ggcgtttcca
    44521 gtggagccgc ctgctgggcg gccctggagt tggccaaaag acgggagaac cgcgggaaga
    44581 tgattgtcgt cgtgcttccg gacacgggag aacggtacct tagcacgtgg ctttttcagg
    44641 agccgaattc gtgagctacg ggcccccatg tcgcacaacg ggtctccccc ggagcgtccc
    44701 gggcatgcgg cggttgcgcg gatcaccgat tcggtttgga atgcagccgg gtgcgacttt
    44761 tggacggatc cgtttcgcgg atttccggtt cttgaaaaaa gggtcggagc tgttggcaaa
    44821 gccccgggag cttcacggcg aagatcaagg ccccggtgat gcacgaggcg gcacccagga
    44881 ggagagtggc ccgggcccct attctctccg caacacttcc ggcaagaatg cttccaaagg
    44941 gcatgacgcc cagaaacgac atggcgaaga ggctcatgat ccgaccgcgc ttgttctcgt
    45001 ccacgatggt ttggaggatc gtgttgctgg aggcgatctg gatcatcatg ccgaagcccg
    45061 ccgtgaagag gcagacgatg gaaagggcca cgtttccgga gagggcgaaa aaggcaatcc
    45121 ccaggccgca cgcggcggcg gcggagggaa tcagcctgat caggccgcgg gcactcctgc
    45181 gggatgcgag gtacatcgtg gccgccaggg ctccgatccc cgccgcggac atgaggaacc
    45241 cgtaggtatg cgccccgccg cagagaacat ccttggcgaa ggcgggcagg agaaccaggt
    45301 acggagcacc ggccaagctg aaaatggaaa ggagggcaag aatcgccagg atgggcttga
    45361 actcgaaaac ataacggagt ccttcataaa actcccgcca gacgggagcc ttcccgttcc
    45421 ggattggtcg aggagcgatc cggatcgcgg ccaaacccgc cagcaccgca aggtagctga
    45481 tgccgttcaa aagaaagcaa atcccttccc ccaaggtgga gatgagaatg ccgccaatgg
    45541 aaggaccgat gaaccgagcg ctgttgaaca tggcggagtt gagggcaatg gcgttcccga
    45601 ggtcttcttt cctttccacc atttgcacca cgaaagattg gcggacggga atgtcgaaag
    45661 cgttcacgat cccgatgaac acgctgagag cgaggatatg ccccgttcga acggcgcccc
    45721 ccagcaccac ggcggccagg atcaaagcct ggaccatgga caggcattgg gtccagatga
    45781 ggatgcgttt gcgctcccac cggtcggcga ccactccggc tacgggtccc agaaccaggg
    45841 tcggaatttg gctggaaaag gcaaccacac ccaacagaag cgtcgatccg gtgattcggt
    45901 acaccagcca gctcatggcc gcctgttgca tccaggtgcc gattagagaa accccctgcc
    45961 cgatgaagaa caaccggtag tttctcgaat ggagcgaccg gaacaggaac ccgactccgg
    46021 aggcttccgg actctcagcc acgggatgac ccacttccac tgcttggggg gttcggcaag
    46081 tgcggggacg gatcgctctt tcggacgatc acgggccgtt tccgtccccc atgcagacat
    46141 tcgcccgcag tgcgtcttcc tccagggcca gggaaacggg tgccgagaga gcggactggt
    46201 ttacgtagac gtcgtcaaca tacacgtcca tgtgtgccgt aggcctggga ctggaagtgc
    46261 aattgggcac gttgagttgc acatcgatgc gtttgttgaa caccgtgccg ggggcggacg
    46321 agtccgacac ggtgtacgtg gaaaagacct ggggttttgt gtcccgctgg aagatgaacc
    46381 gcttgttggc cttgtcccat tctatgtaga gggtaacctt ggagcccacc ttgatcgttc
    46441 ccaattgcct ggtatccaac ggagtgaagg tgtagcagtt gtcgtcctgg cattcgacga
    46501 tggtcccaac cactctcagg gtatccgggg cgtccttgga atcggattta cgttggatga
    46561 agatttgggc catgacgtcg tgctggctgc tccactgcgt cggggtgccg gtattgaaga
    46621 agaagcccga cagacgggcc ctggcgttgg cggggacgga gttcccggcg catcccttgg
    46681 acgtgtagcc gtttacccaa aaggtggctt tcatggcctt gatgccgtcc ggccgggtga
    46741 ataccaggcg attgcctccc ttctcgtctc cgctatcgga gcccatcccc gcataggccc
    46801 ggctcatcaa tcgcaactgg ttgccaaggg tggagtgttg ttccacccgc cgaagatttt
    46861 cacgaaagaa gggggcatca aacttttcga ccccaaacca cttggagggg ttgatgagat
    46921 ttgccttgaa gttgtcgtaa aggaccaggg gctctgcagc ctgcagggcc gtcccggaca
    46981 ggagaagcag acacaatact gcgcatccaa gaatccgttt catcctcttt tctcctttgc
    47041 gtcctttgag aacccgtgtt gggaccagac gtaacgagat gggaaattgc gacttgtcgg
    47101 ctccccgtac caaaacccgc tctcttcata caccaggaat gtagagaatt cgacgagaat
    47161 cttttcagtg gggaaagatt ccaacgggct ttgggaaaac ggcttccccg tcgccctgaa
    47221 tcgggttccc caccgagaca ggcatggatt gctcccattc aaaaacccct gtcggggtcc
    47281 atttcccagg ggggcggcgg agtcttgcgc gttgtcttgg aggcaggcgc aggaaggagt
    47341 acctgcctcg tcttgtgggc ttcgggtctt ccggtaccgg cagaatcatc caagagcggg
    47401 atttccgcta cggcggggtc cctcgggttc gaaaccgcat gttcctgcgg cgccctttct
    47461 taaagtcctc gcaggtttga tccggcatag aaccacagga acgccttctt gctcaacaag
    47521 cacaccacta ccatttccag aacgaaggaa gccgtggtga tgcacttcaa catggaggga
    47581 tgggatcgat tgacgggttc cttcagaagc cgaagggtca ggttcaggat gccccctcgg
    47641 accacataga agggaactcc cacgaggatg gccgttacat acatccccgg gacgagaaag
    47701 agaaaggccc ttgcggtgag tagggagtgg atgccgaaaa aggcgatgtg aaaatacagg
    47761 ccgaaaacca caaagaccag accggtaaat gcgccgggga cctctcgccg tctttcttcc
    47821 ggatcatcca tggttgccat tgacggatcc atcctgatcc aaattgccgg gaagccattc
    47881 cacattttac gggtcgcagc aaccggaaga ttgttctctt gcggttaaga ttgactgaaa
    47941 tggagtaccg acgagcacgc agaaagttga tatcttctac catttcaagc tcggttttac
    48001 cacagggatt tccacctctc cccgggcttg agcgtttgag gggcatgatt ttgggtcctt
    48061 tcagtctacc gggtcgcgcc acccctgctt tcctgtgtac attccctttc cgcgtcggcc
    48121 cgacgcatgt ggcacaagag cattttgttc atggtgattt gtccgtcaac gacgaagcct
    48181 tcccggacat agaattcgta ggcgggcgtg tcctgcgcaa catccaagat gactccgtgg
    48241 tatccaaggg ttccgcagaa accgtacagg aagcgcagca tggtcctgcc cagaccttgt
    48301 ccgtggaaag tcgggagggt ccccaggagc aacagttcga gatagggaag atcgtatttg
    48361 ggacgccggg acaaggaagt cataaactcc cagcccgtcc tccatcccag cagacaaagc
    48421 agacctccaa agaagcgaag catggccttc ggtccgggtt tgaaacgggg gtccaacgag
    48481 aaggccgcgc agaccagcgt gccgtctcgg cggatgccgt ggagccatgc ccggttgctg
    48541 ccggcaaagg tcttgatgaa gagttccagg agcgcccgcc tcgtttcggg cggtgtgtct
    48601 cgaggcagca cagggtggtg ggcaaacgcc tcggtgagca ccgccagggc ttcccggtcc
    48661 ttgaacgggt cgagagtttc gatcttgatg gctttcctcc tgtgggaatt ggaacggctt
    48721 cgtggttttg gcccatcagt ccgccggggg ggccgatttc acttcggccc ggaattcgcc
    48781 cgggtatttc gattgaccgt gaggagagag gaggccttct cccgtaggag gaagccgatg
    48841 aggggctgtg gctctatcgt tccgggtgtg tgccggggac cggaaaaacc aggtccccgt
    48901 attctcagcg ggttcgcgac gatttgtcgg gctcgacggc tccgctcccg ttggcccggc
    48961 tcctcgagtc gaacggcatc tcgatggacc gtcggggggc acaactccaa atcgatttat
    49021 gccgttcccg cgtccgccgg tgatgttgtt ctcgaggcta tctgccatgt ctttgacgct
    49081 cttccttctg actctcgtgt tgtctctggc gttcctgctt ctggcgcatg cgttgctctt
    49141 gacgctgttc tttctgcctc atttgctgct tttctcgctg ttgtttctgc ttctgcgcct
    49201 tgggttgccc ttcccggtcc tgtcgttttt gcttctgctt cagttgctgg tcctgcttct
    49261 tttgcttctg tttcagctgc tgatcctgtc gcttctgctt ttgtttcagc tgttgatcct
    49321 gcttcttttg tttctgggta atcgcctgct tctgctgctt ttctttggcg gcctgacggc
    49381 gataggaagg atcatgtttc aatttcattt cacgattcca gctggccgtc ctgacatcgt
    49441 tgatccggat gtctctccgg ttgatgttga tgttccgatt gacgttgacg atgatatccc
    49501 ggtgtcccca gtcccaacgc ccccatcccc agttccatgc cgaaccgacg gcgatgcccg
    49561 cggcaaaccc aaacaccccg gccgtaacga tgcctccaac cgggtagaac ggggagtaga
    49621 cgtacggagg ataggcggga taccaccagg gcccgtagac ttccaagggg ttgtaggtgg
    49681 gcacatagac gacccgggga ttggccggga cgatttcaat actttcgccc ctgaccacaa
    49741 ccttttgctc cttggtggac ttgagattgc ctttggcttt ggccttggct ctcaggctct
    49801 ggatggtgtc catgacctgg ccctcttgcg ccatgaaggc gtcgcccaac ttcttggtcc
    49861 attccggctt ctcggccatg agggcaagga cctggggaaa gggtaccagc gccttgacgc
    49921 tcagatccca gtccttttca tccagggcgt cgttgagctg attttccgtg agattcctgt
    49981 tgtgttttag ccaaaggtcc gcttccgcca cttcggcggg ataggtcgcc gccataagaa
    50041 tctgggccag cagcgagtca gggtagaggg cgatgggtgc cagcatttga tccagttctt
    50101 cctgcctgta tgggccggcc gatcccgaac cttgggcgaa tgttcccgcc ggggcggcca
    50161 gcaccaagac gagaaacccg atcatcacgc gcatgaagat tgtctttgtg ttcatcgttt
    50221 ctccttgttt gcaatggatg tttcacggca ccgattcatg ttgccaccac ctccacgcca
    50281 atgggtcacg ctcctttctg ataggtgtcc gattttagtc cttctccttt cctagcgcag
    50341 ggggttggaa gtttcagctg cccggcttct cccggaccct gccggctcca acccttatcc
    50401 aaccgtgatc cgcgttcgcg cgtcatggca gggggagaca gactcccccg attcctcttg
    50461 cgcacattga ggtccccatt ctcctgagtt gagcgactcc gagaacatca aggcgtcatg
    50521 ccccccaagg cgggcatcca gggggtatgc actctccttc cggattcccg cttgcgcggg
    50581 aatgacgaaa cgggattccg accggccaaa tcgctcaatc caggattctg ttgccaatgt
    50641 accctgcccg gatacccgga cacaagtcgg acttgatttc taatgacgtt tccgggtcaa
    50701 ggaaagtctc accaaaatga gaatcctttg cggggtgagt ggccaggtcc tcggcgatcg
    50761 aggtccgcga gcgaatccgc tcagggtcca gtcggcgccg gtgcgagggg gggaagagct
    50821 gcgaaaggat caggcctcag ggagaatccc ggtcgagaca cagaaacagc gcctcccgat
    50881 ccaggaaatc ggcttcgctc cccaattcgg aaaaaccttc ccggtccagg ctccgccaca
    50941 ggtccccatg agtgcagccc ttgaggcgga caaggctcaa gcggacgcga ctcccgccgc
    51001 ttccccgcct tggttcgcac cattgttcca tgcgggtcgt caaggccgat ccttcccgca
    51061 ccagggtttc caggagcagg ggatcttccg ggtcatccag gaacccgtcc aatgccttcc
    51121 agaaagattc ccggataccg tctctatcga gttcctcttg cagggagccg aatgcctccg
    51181 ggctgagaag acccggggaa ggcatcccgc atagggggac ttccatgggc ggggcatctt
    51241 cgccgcgctt ttcccgctcg ctttcgagcg gttccaggat ttcttcgagg agctgtcggg
    51301 agaagatcgc cgggacccac cccggttccg ccgggccttg agacaggagc cggtaccagc
    51361 aggaggcccg tttcaggatc atgtaggaaa aatccgcgtt tccggaaccc agggagggcg
    51421 tgatcacctc catggcttct tccgtcgttt cactgggctc gattcccagg atccggcgga
    51481 attcgtcttc gagcttctgc gcctcgagaa ggccggatgc gatctccgat tccctttcat
    51541 cgaggtttcg ggcagtttcg aggaagacgg ccgcctcgag gagacagcgc ttcttgaggt
    51601 cttcctcgtc gctttcttgc tccttgattc gtccccggat ctgaaagcgg ctttcttcct
    51661 cttccccggc cagggtactc cggctcctgc agaccaggag cccggtttct ccctgccgct
    51721 cggcaaaatc caaataaccc ttgaggtaca acccgatgcg ttccaggtct tcggggtcgt
    51781 gaatcgtcgg ccggctccgg accttctccc aagcgtagga gggcacggca ggacctcgca
    51841 gcacctgaaa gatctcgagg cggggaagca ggaggcacag ggcgcgaagg tcctcctccc
    51901 tcaaaaggag atggggaaag agcaaatatg gatgttccat ggatctcaaa tccttctgtt
    51961 gcaggatcca tcctcagtcg gtctccccct cccggttcca ttcgcccccc gtgggggatt
    52021 cgcggggacg ggcggtgcgg agtcccgggg agagggcctc aatcctcctg atcccagaag
    52081 agaggcttgt tcgggagggg aatattttcc agcagcgatg cctcccgcaa tagagttcgg
    52141 acggcccgtt ccccttcctc tcccacatcc aggctgaatt cgttcacgaa catctcgatg
    52201 tgcctctcga tgacggatgg ctccatttcc tgggcgtgat cgcgaatata gggccagact
    52261 tcctcggggt gattccgcgc gtggttcagg ctccggcgaa tcctttcctc caccgtccgg
    52321 gcaatctccg ggcccagctc tctccggatc aggatcgccc ccaggggcag aggaacaccc
    52381 cagcgggatt cccaccagga acccagatcc aggaccgatt tgagccccat ggacggatag
    52441 gtgaagcgac cttcgtggat gatgagtccg gcatccactt ctccagcgac cgttcggggc
    52501 atgatctggt cgaaaggcat ctcgaacacg ggtccctggt gggatccctc gaggcgaaga
    52561 agaaggtggg cggtggtgag cttcccggga atggccaggc tccggcggcg caattctccg
    52621 ggggaaaggg ccgcgcggga tacgaccagg gggccgcaac ctcggcccat ggctcccccg
    52681 gcccgcagca tccagtagtc ttccaacagg cggacgaccg ccgagaccga taccttggtg
    52741 acatccaact ccccggcccc ggcctttcga ttgagtactt ccacgtctgc catgaagatt
    52801 ttgaaggcga ggggactcgg gtcgattcgg ccctcggcca acgccccgaa gatgaaggtg
    52861 tcgttggggc agggtgaata tccgaggctc aaagtccggg tcatcggtgt cctcagctta
    52921 accaggtgcc caccaccgcc cggcagtggg acagggcctt ttccgtctgc cagcgatcct
    52981 tgaagcgatc tcccgccgtg ttgctgattc ctcggcattc cagtaccgga actccaaagc
    53041 gcaggcaagc gtgaataacg gcgctccctt ccatattttc tgcgtaaact gaaaaccgct
    53101 ccgacctcag ccgggccgtt tcctcgtccc cgctcaccat gcccacggta aggctgggtc
    53161 cgtagacaat gcgaaaggag acgggggaac agccggtggg tgttcccagg accgggtgcg
    53221 gggaagcgga cttgcagggt gcggcccggc ttccggcagc catcgaacct acatcgaggg
    53281 tgtaacagcc cggtggggtt cgcgcccgga ttctttccat gagcggctgg gcgtcaagcg
    53341 gaatggagtc gaagcactcg cggcctccca cctccaggac ggggatcccg atctcacggc
    53401 tcgaaagcac cccctctcgg gtcagaaccc cttcgtctcc gcaccatgcg gtttcggtga
    53461 ccagcacgtc cccgaggttc aggggagaat ccacgtagca ccccgcagta ccgacgttcc
    53521 agacctcgga caccgacaac ccctggagaa gggccgccgt agtgatggcg gcgttgactt
    53581 tcccgagacc cgtggttccg atgaggaccg gccgtcctcg gtggttgccc gccaggaagg
    53641 gacggcctgc cacggtaagg gatcgcgaac cttccagcag gtcgaagaat ccctcgactt
    53701 ccgcagggac cgctcccaga acggctacat ggaatttgtg cactcccgtc atcacctgtc
    53761 cgtgccgggg agactccccg ggggcgtcga agccggacct tcgcccatcc ccggggccca
    53821 ggccgccttc gcgttcatgg gggtcccttg ggcggcccct tcggtgtcga accagaacgg
    53881 atcgcccatc gccgccgaag ccaacccctg cggcaggggc cccgtcatct ccgggtccca
    53941 gcccgaggga gggaacgttt atttctcttc cccggggctc ccattgactg aaccacaaaa
    54001 gcggcatgag cacaagagct cacgtgaaga gacccggatc caatttcggc ccagtgcagc
    54061 cggagtttcc ctcgactcaa tccgagaaga cctctgtgga ttgccatgag aaccggaact
    54121 tgctaggatt accgctcttg gaagaccatt ttggggttgg aaaggcaggc tgcatgcacg
    54181 accaagagca aacggcaccg ttccggccgc cgccgctgaa gatcggccct ctggtggtgg
    54241 acccgccttt ggtccttgca cccatggccg gggttacgga cagcgtttat cgatggatca
    54301 tggcggaaca tggggtggga ctggttttca cggaaatggt gagtgcccag ggactgctta
    54361 gggggaatcg ggaatcggtt cgcctttgcc ggcaggatct tcctctccca ggcccccttt
    54421 ccgtgcagat ttttggaggg gatgtcgacg tggtgggaag ggccgccgcg agcctggaag
    54481 ccctgggcgc ctcggccgtg gacatcaacg cgggatgccc agtgagaaag gtgttgcgcc
    54541 agggggccgg ggccggactt cttcaaaagc cggacctgct ggcccgcatg gtggagaaag
    54601 tcaaacgatc ggttcgaatc cccaccacag tcaagattcg actggactgg actcaatcct
    54661 attccgccac cgtgaacctc gccaagcgac tggagtccgc gggtgccgac gccattaccc
    54721 tccacggccg cacccctgct cagcaatttc ggggagacgc ggactggacg gccatcgagc
    54781 aagtcaagaa gtccctggga atccccctca ttggaaatgg agacatcacc cgaccgtccc
    54841 aggccgaccg ggtgcttcgt gagggcaaat gcgatggggt gatgattggc cggggcagtc
    54901 tgggaaaccc ctggctcctc tccaccattg tctcccggtg gcgctccccg gttcaacgaa
    54961 atccccatcc cggctgggag gatttcttcc gtacggtgca cgaccatgtg cgggcattca
    55021 gggaaaagtg gcctcattgt atgggccgat tgaggaccct gctcatgtat tactcgaaag
    55081 gctttccgga cacgtgccgc ttgagggccc ggctgtcgac ggcggatggt tgggacgttc
    55141 tcctgtccgc atttgactcg tggatggagg acatcgcccg acgaggatgg gcgttcctgc
    55201 cgttcaagat ccctgaattg aagaaaggtg aggaaccatg aaggtcatcg tcgccaagac
    55261 cgccggtttc tgcaaggggg tgaaggacgc cctggaagtg actctcaacg ccattcagca
    55321 gcgcaaggag ggagagagga tctgcacttt cggacccctc atccacaacc gccaggtcct
    55381 caagatgctc gaggaaaagg ggatccagga ggtggcggac atcgagaact gtgcgggaaa
    55441 gaaggtcgtc attcgcgccc acggaatccc gccccgggaa cggcaggccc tgcacaggat
    55501 ggatgcccgg ctcctggacg ccacgtgcaa gagagtggcc aaggtgcatg ccgtcatcaa
    55561 gaggcacgcc cgccgcggat accgtacggt catcgtgggg gatgcggacc atgcggaagt
    55621 gatcgggttg atgggttaca cggaagggcg tgggatcgtc attgatcggg tggagcaggt
    55681 cgaacagctg ccccgggatt gggaaaaggt gctcctggtg gcgcaaacca ctcagaacga
    55741 ggagaccttc cgggaaatca cggaagcctt tctcaaacgc tatcccaagg gcattgtgaa
    55801 gaacaccatt tgcgggtcca ctcacgaacg gcaggcggaa gtgcgggaga tgtgctccca
    55861 ggtggaagcc atggtcatcg tgggcggata tcacagcgga aatacggtgc gactggtgga
    55921 agtggcccgg gagtgtgcca ttcccaccta ccacgtggag acggaagcgg acctgaacca
    55981 gcaggacatg gaacgttact cctgtgtggg aatctctgcg ggcgcctcca ctcccaattg
    56041 gatgattcgg agcgtggtcc actacctcga gtccatccag acccggaaga tgagccgcaa
    56101 gtatctcctc aagagggcca tggaactcct tgcctacgga aacctataca ttgccgtgag
    56161 cgccgcactt ttgccttccg ccatgcaggc catgacgggt tttccgggtt cctggtacga
    56221 tggtctcatg gcggctttct atgcctttgc catgcattcc ctcaacatct atctggatcg
    56281 aaacgccatc aagctcaacg accccagccg ggcggccttt tatcagcgat ggcgcctggc
    56341 cttcaccggc gccggagtgg cggccgtcgt ggcggccctg tggattgccc ttgatgtggg
    56401 attcttggcc tttctggtga tgggggtcct ggtcctcctg ggaatctctt acgccgttcc
    56461 cgtatttctc ccctcgtcct ggcagagcct ctatgcgttc aagctcaagg acatccccac
    56521 ctccaagacc ttctccgtgc ccatcgcctg ggcctccgtt acggtcattg tgccctacac
    56581 ggctcatgcg ttgcccggat tggatcgggc gatctttgcc ttctggatcg cattcctgat
    56641 ggtcctggtc cgaacggcca tgctggacct gctcgccgtg caaggagatc gactggtcgg
    56701 cagggagacc ctcgtcgtgc tcatcgggga ggccaagacg ggtcatttcc tggtggggac
    56761 cctcatcctc ctgggcctgt ctctggtggt cggtccgtgg gcgggtttgt gcacttcctt
    56821 ttcctacgtg atgcttccgg ccgtggcgct ctattggagc cagctgaaca tgtgctttca
    56881 gaatcgcctc aaagaggacc cggcttttgt gtccgccatc gagtcgacct tgatcggtgt
    56941 ggggttgctg gggctccttt ggaaccttgt ggtgcagtga gtccctgccg tgcgggaggg
    57001 aagaatcgtg agacgaacca tggagaggag accctgacgc atgagagaat ttgaccgggc
    57061 agaactggaa caattcgacg gaaaggacgg caagcccgcc tacatccttc atcggggccg
    57121 agtattcgac gtcagcgcca gcaagctttg gaagggggga ttccacatga ggcgccatca
    57181 cgcggggacg gacctcaccg tggatcttca cgcggccccc catggggagg aggtcctgga
    57241 ccgctatccg caggtgggga ttctcgggga aaaaggaccg gcgcctcgat cggcctccgg
    57301 tctattgagc ttcctgctgg aacgctatcc catgttgagg cggcatcccc atcccatgac
    57361 ggttcacttt cccattgcct tcctgatggc cgttcccacc ttcaccgtat tgtctttgct
    57421 caccggcatc gagggcctgg agaccaccgg tttccattgt ctgggagcag gccttctctt
    57481 cctcccggtg gtcatggtga cggggatttt ttcctggtgg ctcaattatc aggcgagacc
    57541 cgtgaaagcg atcgggatca agctcgcggc ctccctggtc ctgctggttc tggccttgat
    57601 ttcctttgtc gggagatctt ccggggggga gaccctggcg agcctgggac ccggtggggt
    57661 tttctatgtt ctgggagtat tggcgttcgt gcccctggtg gtcaccatcg gctggtatgg
    57721 ggcaggcctg acctttccca cggagtaaac gcagacggtt cggttggcct ccggtcttcg
    57781 gcgatttgcg gaacgcagac ggagcagggg ggagcggtgc ggaagcccgg ccgacggtcg
    57841 gggctttgct ccccgctctc cgttgaaatc ggcccgtgca aggtttatag ttatccgaac
    57901 ttcatggaat cggacgaaaa atgccctgcc ttcgaggata ctttcatggc agatgatttt
    57961 aggctcccta accaggaaga gctggaaaag gaactgagcg aatacctgtc caagaaatac
    58021 ggataccgca tcaaggtgat ttctccgatg atgatggctc aagccaagga atctcccgac
    58081 actcctactc cttcggagcc cacaggcgtg gacaaaatcc ggttcgatct caagcctgag
    58141 gaactggcgg cctaccttga ccagtacgtg gtcaagcaag acggtgcaaa ggccgtcctg
    58201 gcgaccaaga tctgtaccca ctatcatagg attcggttcg agaagaaacg gggacgcgcc
    58261 gaggaagcat ccatggtggg gaggatcaag aacaacatcc ttctcatcgg acccaccggc
    58321 gtcggcaaga cctacctggt gaagctcatt gcccgcaagc tgggggtgcc cttcgtgaag
    58381 ggagacgcaa cgaaattcag tgaaacgggc tacgtgggcg gggatgtgga agacctggtg
    58441 agggagcttc tcgtggaggc caaggaggac atcgaactgg cgcagcacgg gatcatctat
    58501 gtggatgaaa tcgacaagat cgcctcctcc cacagcctga tcggtcccga tgtctcccgg
    58561 acgggggtgc agcgggcact cctcaagccc atggaagaaa ccgaagtgga cctcaaggtc
    58621 gcccacgatc ccatttccca gctgcaggcc atcgaacagt accgcaagac ggggaagagg
    58681 gaacgacgcg tcatcaacac gaagaacatt cttttcatta tgagcggggc ctttaacgag
    58741 ctggcgcccc tggtgaaaaa gcggctgcag aggcaggaga tcggattcgg ggcggaaatc
    58801 tccagcaagg gggacgacta ccgctacatg aagcatgtca aggcggaaga cctgatcgcc
    58861 tacggctttg aaagcgagtt cgtggggagg ttgccggtgg tggccgtcct ggagcccttg
    58921 ggagtcgagg acctttacga ggtcttgcga aacccgaaca atcccgtcct ccttgccaag
    58981 aaggaagact ttcgcagcta cggcattgac atccggttcg aggacagggc cctgcgtctt
    59041 ttggcccgga tgggctacga ggaaaaaacg ggggcgcggg gtttggtgag cgtcattgaa
    59101 agggttctcc tccccttcga gaaaagcctc cccagctcga gcgtcaagac cttggtggtt
    59161 accgagggcc tggtgatcca tcctgaggag gagttgaaga gggtcctggc aaatcccgaa
    59221 gatccggacc gcctcgccgt ctatcgagcc atcctcgagg aggaaaaagg cagggcccgg
    59281 gtgcagctga gtgaaacgca caaccactac gcccaggaat atcccctgat ctttacggga
    59341 cctcgaatgg agattgccct ggaacatcac attcggttcg gttggtccat cgagagcgtc
    59401 ctggacgaaa tgatgcagct ttacaaccag gttcgcgtgt ttgaagggga tttttgtgaa
    59461 acccacggct tccagatttc ctttgacgag gaagcggtgg acgaaatcat tcaagaagcg
    59521 ttgaacacgg gaacgagcgc taccggcatt tgtgcaaggg tttccaggga ctacgactac
    59581 gggttcaagc tcgtctccga gcgaagcggt cagctgcggt ttgttctacc tagggacgcc
    59641 gtccttcaac cggaccaata tctggacgat ttgattcggg acagctaccg ccagtatccc
    59701 atcaccctgt ccgaccgcca acaagaatcc cagaactaag aggaaccttc atgatcggaa
    59761 tctccaagct ctactgcggc acggtggaac cctccgatgc cttgcggtat ggtcgcctca
    59821 gcggtcggct gccgtcccat ctccttcagt tttccaccga caagcagccc gtagtagtgt
    59881 ggaacgtcac ccgccgctgc aatctcaagt gcgtccactg ttatgcccat gccaaggacc
    59941 agcccttcga aaacgagctg agcacggagg agggaaagcg ggtcctggac gacttgagcg
    60001 agtttggatg cccggtcatc ctgttttccg gaggggagcc tctggtacgg cccgacctgc
    60061 cggaactggc ggcctatgcg gtccagaagg gaatgcgagc ggtgatctcc accaacggca
    60121 ccctcatcac tcccgccgtg gccgcggaac tcaagtccat cggtctctcc tacgttggaa
    60181 tcagtctgga cgggatggaa gaggtcaatg atcggtttcg gggcgtcaag ggggccttcc
    60241 agcgtgcact catggggatc cgggcctgcc gggaggaggg gatcaaggtg ggacttcgct
    60301 tcaccatgaa tcgcctcaat gggttcgaaa tcccttccat tttcgacctt ctcgaggaag
    60361 aagacattcc ccgggtttgt ttttaccacc tggtgtacgc ggggcgaggc agcaagctca
    60421 tggaagagga cctttcccat gaagaaaccc gagcggccat ggatctcatc atggaccgga
    60481 cccgggacct gcatgagagg ggaaaatcca aggaggtcct caccgtggac aaccatgccg
    60541 acggtcccta cgtgtacctg aggcttctgc gggagggttc tcctcgagcg gcgtcggtgc
    60601 tcgagctgct ccggatgaac gaaggaaaca attccggcag gggaatcggc tgcatcagct
    60661 gggacgggag ggtccacgcc gaccagtttt ggcggcacta ttcctttgga aacgtgcggg
    60721 agcgcccttt cagccgcatc tggaccgacc tgtcgaaccc cctcatgaag cggttgaaaa
    60781 acaagaaaga atacgtcaaa ggccgatgtg cccgatgtcg ctggctcgat gtgtgcggag
    60841 gcaatttccg ggtccgggcg gaagccgccg tgggggattt gtggttgccg gaccctgcct
    60901 gttacctgac ggacgaagag atccgctgag gatggaaggg gaatccatga attttccgat
    60961 ttatcggccg aggcgattcc ggcgcacgga gaatctgcgg cgtttgattc gagagacgac
    61021 gtggtccgtg gaccaattga tctacccgtt gttcgcggtt ccgggcaagg gagtcctgaa
    61081 tcccgttcaa tccatgcccg gagtgtttca gcgatcggtc gacgaactcc tgcgggaagt
    61141 gaaaaccgtg gtcgacttga aaatccccgg ggtcctcctg ttcggtctgc cggaatccaa
    61201 ggatgaacac ggcagccagg cccattcccg ggaaggggtg gtccaggaag ccgttgcccg
    61261 gatcaaagac aagtttcccg acctcctggt cattacggac gtgtgcctgt gcgaatacac
    61321 cagtcatggg cactgcggaa tcctgcgggg ccatgaagtg gataacgacg ccaccctgga
    61381 cgttctgagc caggtggccc tctcccatgc ccgggcggga gccgatgtgg tagcaccttc
    61441 ggacatgatg gacggtcgtg tgggatccat ccggacctgc ctcgacgaga acggattctc
    61501 ccaggtcggc gtcctggcgt actccgccaa gtattgctcg gcgttttacg ggccttttcg
    61561 ggatgcggcc gactcggctc cccagtttgg ggatcgcagg gcctaccaga tggatttttc
    61621 caacgccggg gaggcccttc gggaagtggc cctggatatc gacgaaggag cggacatggt
    61681 catggtcaag cccgccctgg cttacctgga cgtcatccga agggtcaaag acgagttcgg
    61741 ctggcccgtc gccgcgtaca acgtcagcgg ggagtactcg atgatcaagg ccgcatcggc
    61801 caacgggtgg ctggacgaga cgcgggtcat gatggagatc ctcacggcca ttcgacgggc
    61861 gggagcggat tgcatcatca cctactttgc caaggatgcg gcgagaattc tctcttaacg
    61921 gacagaagcg aacggggatt catgaatcac ccttggggaa aaaaccacga gtcgaaaaag
    61981 gctcaccctt ccggtcacgc ctcgggccgg gctcccgccc tgcgcctggt ggcatgggaa
    62041 gtcactcgca cctgcaacct ggcgtgcgtc cactgtcgtg ccgcagccca ggatcgcccc
    62101 tacgaaaacg agctgaccac cgaggagtgt ctcggcctgc tggacaatat cgccaccttt
    62161 gccgatccca tcattatcct cacgggcggg gagccccttc ttcggcccga tatcttagaa
    62221 atcgcggcgt acggaactca aaagggcttc cgcatgacca tggccgtcaa cgggaccctg
    62281 cttaccccgg acaaggcccg aaggttgaag gaagtgggca tccggcgcat tagtgtgagc
    62341 ctggacggtg cgacgtccgc ttctcacgac gcctttcgac aggtgcccgg cgccttcgag
    62401 ggggccctgc ggggcatcga aaacgccaag gcggaagctc tcgatttcca gatcaacacc
    62461 accatcaccc ggcagaacca ctccgaactg ccggccattc aggaactggc cgtctccctg
    62521 ggtgcgacgg cccaccacat cttcctgctg gtccccatgg gacgaggcaa agacattgcg
    62581 cagcagggga tcgatgcgga acaatacgaa aagaccctgc attggttcta cgatcaaagg
    62641 gaccgggtcc ccctccggct aaaagccacg tgcgctcccc actactatcg catcctgcgt
    62701 caaagggcca aagccgaagg aagggaggta agcccgcaaa ccttcggtct ggacgccatg
    62761 acccgggggt gcctgggagg caccggtttt gtcttcgttt cccacgtcgg ccgggtccag
    62821 ccttgcggct acctcgagct ggattgcgga aacgtccggg agaagacgct ttcggacctg
    62881 tggcaccatt ccccgatctt tcgggacctg agggaattca ggaaatacga aggcaagtgc
    62941 ggcgggtgtg agtatgttcg ggtctgtgga gggtgccgtg cccgggccta cgagagcacg
    63001 gggagctacc tggccgaaga gcccctgtgt ctttaccggc cggggcaaaa gacctgaggc
    63061 gatcctgttg gaaggatccg gtttcgggaa aggaaggggt tctcaaggtc gagaaaaaga
    63121 gaggggcatc tataggccga tggacaacgt acccggcaaa aacaacgacg ttctctgtcg
    63181 ggcagtgcgg cagagtctgg gtgttctggt ccttgcggtc ttgatcgggc tgagcgggaa
    63241 cgctctgcgt cacgacgggc ttccctggat aggcgactgg tctcccccgg cccaactggt
    63301 ccgctcgggc gttccggaag ggatgatcat tgcgctggac gaggctgcag cgctgtttca
    63361 tgcaggcgga gcggttttca tcgatgctcg cccggaagag gctttccggg cagggcacat
    63421 cgccggtgcc aggaacctgc cctggcagga tttcgaaaac cgcttctcgg aagccatgtc
    63481 ggaccttccc cccgacagca tcctgatcac ctactgcgac ggagagagct gctccttgag
    63541 caaggatctc tcccttgcct tgttggggaa ggggtatcct ttcgcgcgaa tcctggtgaa
    63601 cggctggacg gtctggaaag aagccggtct tcccgccgaa tagaaatcgt ctcaagaccc
    63661 cgtttccccc aattcccgat tccggaagcg atcacgaagc ccgcgcccgc atccaccctt
    63721 tccggccgca aatcgcgacg gctactttcc tttctcttcg gaacggggcg gtccctggtc
    63781 cccggacgag tttcgaggcg agctgccggt cgccttctcg ctgcctttgc tcccgttcct
    63841 ggatggggcc gcgggagggt ccgcaatgtg gagcaactga acgtggactt cgtgttcttc
    63901 ctgcggttcc acgtcggtct cgatggtcaa aaccagctga gtcctgcttc ccggagccat
    63961 gttttccaac agagggacaa tctcgaggct gaaacccgca tccttgggca ggggctgccg
    64021 ggtgacccgc agaaggcttt catcgtaggt aagcttcgtg atctggaagg gtcgatcctt
    64081 gttgctcacc accatcacct ttccggtacg ggggggctgg tggggaacaa gcttcccgat
    64141 cagcacgctg gggggacgga ctccgatctc gccctccacc gaaccgttca cgcgaatcag
    64201 gatctcgggt tttcgcgggt gatcggtgcg gatcttgaca aacccgctat aggtgccttg
    64261 cttcagacga ttccggacct ttagctggta atgtttcccc tcttgaacgg tctccaggct
    64321 gtactggatc ttatcctgga gattggtttc cacgctcccg acgttgaacg tttccgtggt
    64381 gctcgacagc tcgatggtct tttcggccaa ttgatcggca acccctcgaa aagcgacggc
    64441 gctgccgggg cgcacctcga tgagggagcg gacaaagccc tgtaccgtca ggaccactcg
    64501 agggttctgg ggatcgttgc tgaaaaccgt ggccgttttc ttcacatttc cctggtaccc
    64561 cttgaggtcc accctcagtg taaccttccc ctcgcctccg ggagggacgg cccgatcaaa
    64621 atgggccact gcacagcctc agccgggtcg gacttgatcg atctggaggg cgtcggtccc
    64681 ggtattcctt atggaaaaat cgtggaccac cacctctccc tcgacgattt ctccaaagtc
    64741 gaacacgggt gcgggaacgg aaagggacgg tgcttcttga ggctgggtcg gagcaggagc
    64801 ggagggtggt ggagggttga cgggcggacc ggcggcgtag gctgccgatg tcagcaagag
    64861 ccacaacaga aacgcacagc tttttctcaa aaacgtcatc ctatctggaa ggtcttcttc
    64921 aacccaatct cccggaattc tttgcttcgg gcctgttttc ccggagatgg gaatccggag
    64981 attttcgttg gatttgcata gcgtttcccc cttcaaacgt caaggtgaaa aagggggcgt
    65041 cgaccgtcct ccccagccat ccggtcccgt tgcggggagg agctttttgc gggttcatga
    65101 ggcgcctgcc gatgaacccc gttgaaggtc ctccgggttc gggtcttcct tccccgtagg
    65161 gctttgccga cttggtcccc ggggcagaag ctcaccggag ggcactacca ggaccccttc
    65221 ttcctgaaac cgcccggcca tatccaggag agccttcagg gttgcttccc ggggatggcc
    65281 gatggccacg gcgctgcctt ccacccgggc ctttcgaatc agtgccagga tctgtgccct
    65341 cacaaacgcc tcggaattct cgtggtccag aaacacccga cgccggagga aaggtacgcc
    65401 gtactgggaa gccaaggcga tgcacacgct tttctcggtg gtgtaactgt ccaagaaata
    65461 gagatgacgt tgacgcaact cggcaaaaag gacgcccatg agctcagcgc gctcggtaaa
    65521 ccggctcccc atgtgattgt tgaccccggc agcgaaggga gtctcatcca gtgcctttcg
    65581 gagggtctgc cgaacggcgg actcggacat ggagaccaga agggctcccg gccccggccg
    65641 ggtcttggga tatcccttcg gctccatggg aaggtgaaga aggacttccc gctgatggta
    65701 gcgggccagg tgggcgatct cccggctgtg ctgttgataa ggaaggatgg agtaggtgag
    65761 gggcagaggc acggccagga atcgcttggc tatttgtaga tcctgcccca aatcgtctat
    65821 gacgatggcg gcccgggcaa gagggagggg agggacgacc ggttgcggag gcggcggagc
    65881 cttctccagg ggccgggatg ccgccgccgg gggaagttga ggcttggcgg ccatgtcggg
    65941 cctttcaggg gcggcgggcg ggaccggtgc cggccgggtt gcctgtgggg cgggggccga
    66001 agcctgtttg ggtgtctcgc gggccttttg ggcagtcccg ccggaacccg aagacctccc
    66061 cttggcccaa taaatcaacc ctgccagggt cacaacgacc atcaaccaga cggcaacgag
    66121 ggggagaagc gccgacggct tcctggggga agcctttcgc gaagatacgg aatcgccgcc
    66181 tcttttgggt ggtttggagg gggtcccgcg gccgatcgag ttccgcaccg cctttcgccc
    66241 cttcccggcc ttggtccttt tcccttgtgc cggcactttt ggtcaagccg ctcgaatgaa
    66301 gtgaggcccg agttacttgg actgcatctg catctgggcc atgatcttgt agcccttgag
    66361 cagatccagc cctctacgga gttgattgtc ctgttcgagc atcttttgta cctccgccag
    66421 tgcttcggat gccttcgggt ctttgggatc cgacttctgc ccttccatat ggcggggcaa
    66481 atccttttcc ctcacccggc ggggatcatc ggggacttcc tcgctttcct tcgccggagt
    66541 ctcccgtttc accacgatat ccggttcgat gcccttggcc tggatggaac gacccgacgg
    66601 agtgtaatag agggaggtcg taagccggat ggccgacccg tcgtttaggg ggatcacggt
    66661 ctggaccgaa cccttgccga aggtccgctc gcccaggatg atggctctct tgtgatcctg
    66721 cagcgccccg gcaacgatct cggaagcact ggcgcttccc gagtttacca ggacgacgat
    66781 aggataattg tggggcttcc cgttcttgtg ggcctcgaag cgcatctgct ggctttccaa
    66841 ccgaccgccc gtatacacga tgaggccctg gtccaggaac tcgtcgctca cctccacggc
    66901 ttggtccagc aatcccccgg gatcgttacg gaggtccagg atgagcccca tgaggggttg
    66961 gctgccggtt tccaattgtt ccagggcttt tcggaggtca ttggccgtgc cgctctggaa
    67021 gctggagatg cgaacatagc cgtatcccgg ttccagggtg cgggatcgaa cactgcggat
    67081 ggaaatcata tcccgaacca gatcgaactc caggggcttg cgctccccct tgcgaatgat
    67141 gctcagcttc accttggtcc ccttggggcc gcgcattttt tgcaccgcct ccatgagggt
    67201 catgtcctgg gtcaactggt cgtcgatttt gatgatctga tccccggcca ggatgcccgc
    67261 cttgtccgcc ggagtgtcct cgaggggaga aacgacggtg agcaccccgt cccgaatggt
    67321 gatctcgatt cccaatcctc caaacttgcc cttggtttcg atttgaagct ccttgtagtc
    67381 ttcgggcttc atgaacgaag aatgaggatc caactctcgg agcatgccgt ttatggcccc
    67441 gtggatgagc ttcttggagt cgactttgtc cacataattg tcctgcacaa tgttcaagac
    67501 gtcgctgaaa gttctcagat attggtaaat atccgggctg tcggcgacgg gcttcgccct
    67561 gagcgtcgac caacacccga gcaggatggc agaacccagc aggaacagga gaagtctttt
    67621 ttgtttcgga gacatgggag ttcctcactc tttcatcgtt gtatgggcgg ccagccaatc
    67681 tttgggatcg agaggcctgc ccccttgtcg tatctcaaag tagagcttgg aaccatcagt
    67741 agaacccgaa tctcccacca aggcaatggt ttcgccgcct tgaacgggct ctcccacccc
    67801 tttgaacacc tggtccaggt gagcaagcag agtgaagtag tggtcaccat gattcacaat
    67861 cactacctga ccgtattcct tgacccaatc tgcaaatacg acgaccccgt cgtgaacggc
    67921 cacgacgggc tgacccttgg gagcaaggat cagtgttcca ttgttgtaca ggaacttttt
    67981 agacgcgccc ggagattttc caaacgttcg cttcaactct cccggagttg gcagcggcag
    68041 ggctttttgc atggcggcga aggggctgcc aaaagggtcc cttccttcgg ctgttccgtt
    68101 gctcggactc gcccccggct ctcgacggtc cggatgcccg ctccgggtct tggaaaccgg
    68161 atcgcccttc ccaatccctt cccccggggg gccgggcgtc ggcttctttt ctgccggcaa
    68221 gtctttgagc ctctcttccc gagcatgacc gtccagggat tcgacgatcc cgtggatttc
    68281 cccgggtctc tcggcgactc gagcaaaaat ctccctatcc cgtcgcatca tccgggatac
    68341 ccggtggacg ttttccggaa aatccccggg ctcactcgcg gccagcaacg ggaaaagata
    68401 ccctatccga ccatatttgt aaaaccacac cagccttttc cgtgcggttt ccgccgcctg
    68461 ttgcgtccga aagtcggact tccccgcctt ttcccgcgac aattcaggct cctgttccga
    68521 cgcccggggg aaccaaccgt atcgatccac cccccccagg atcaaaagca ggaacacgaa
    68581 aaacgacgtc caaagcggtg ctttccccgg gtaccttatg atgagaccct cccccacggg
    68641 ccatagagcc ttgctcccag ccagcctcct ccaaccccta ggacggcccc ggtcaggatc
    68701 aggcccagca tcagcaaaac ccccttccag aggtccagag tcaccatgcc cgagatgggc
    68761 aacggcatca ccttttccgc ccggaggacc agcaaacccg ccgggacccc tcccatcaat
    68821 gcccctgccg atcccagcag aaacccctgg acatagaacg ggacttttcc aaaaaggaac
    68881 gtcgcgccca gaatctcata ggcttccagt tcttcccggc gggattccac agacgatttc
    68941 actgcgtgga ggatgatgaa aaagatcccc agggccagca gatacataag cccggtgccg
    69001 gcaagccgca aaccgcttgt ggcggattct attctttcca cccagctttt tccatagaaa
    69061 acttcctcca ccgcaggctg gcggcgtaca cgatccagga cggcttctat ttgttccccg
    69121 tgacgaacct tgggtttgaa agcgatctcg atggtggtgg gcaaaatagg gtcttcaatc
    69181 cccgagagga tttgcttcca gcctcccaac tgatgttcca gccgcttctt tgcctcttgg
    69241 gacgagagga tccgaacctc ggcgatctcg gaagcgccct tcaggaaccg aatcagcctt
    69301 tgctgttcct cttccgagga ttcgggcaac aggtacaaag tcgccttatc ggaagtccac
    69361 cacaggggaa gggattggcg cagggccaag gcaccggcgg ccagcaatcc ccagagaaag
    69421 aaacaaaggg tgagagccgc aagggctgcc gaggaggcaa accagttacc ccccacaccc
    69481 cgcaacgagc tttgggccag ataccgatag cgtcgctcca acacgggtca gcaacttccc
    69541 ggccgggaag aaccaaagat cttccgcttc cgtttcaagt cattcctcca cgatctcgcc
    69601 cccctgaagg gcgaacactc ggctgcccgg gacctgttgg gccagagtcc ggtcacgagt
    69661 cgctatgagg atggtggtgc cccgggcgtg aaagaacttg agcacgtcaa gaacatttag
    69721 cacggccagg gggcccaatg ccgccgtcgg atcgtcggcc accaaaagga gggggtcccc
    69781 ggcaagggct cgggcaacgg aaaccacctg cttttcccct gcggagagcc gggagcagag
    69841 aacgtccttc ctgggctgga gattcaggag cgtggtgagt cgttgcaccc tttcccggat
    69901 cagggcgttg tcttttcccg aaacatagag agggagggca atgttttggg agaccgtctg
    69961 ccaggaaacc agcttcggct cctggggaac caatcccatg gcgtgccgga cggccgcccg
    70021 ttccttaggt gaggcccggt ggagattctt tcctgcgaaa atcacctgcc cccggctcac
    70081 cggctccaat cccgtcagca ggcgaagaag ggtcgttttc ccacttcccg tttctcccac
    70141 cagaaacgcc cattcgcctt ttccgatttc aaggcaggcg ttctgaagga tgggtcccgt
    70201 tcccggatgg gacttgtgga gatgaaggca ttgaaccatg gcccgagaca tcgagaaggc
    70261 gcccgtaccc gagggctcgt gatcggcggt tcgctcccat gagaacgcac cggtggtgcg
    70321 gggcgtctcc ccgttctcga tcttcgactt ggtggatggg caggactcag tcatgaacgt
    70381 ccagcggttc ggtccatgaa acgggtcgct gccggcaaat cagacgaacg attgaccggc
    70441 tgctcgactc tccgtctcga gctcttcggg ttcaatcttc cttcgt
//
LOCUS       DAOOVC010000003        66929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_229_length_66929_cov_5.979319, whole genome shotgun sequence.
ACCESSION   DAOOVC010000003 DAOOVC010000000
VERSION     DAOOVC010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 66929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66929
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229_length_66929_cov_5.979319"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLM79_00640"
     CDS             complement(<1..732)
                     /locus_tag="PLM79_00640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV84838.1"
                     /translation="MNVTSNSTFETGTVIEKKWVILSFIGKGGMGEVYRAHQLNLDRD
                     VAIKVISQGWLREIGDNAYEAETCLDRFRREVKVMAQVRHPNVLQIYDHGSVSIKKGE
                     RDVAVEYLAMEYIPGGTLRSTMSAEGFEPDEERTREWIARYFIPVLEGVRALHEMGIV
                     HRDLKPENVLLDGSAPKLADFGLARSCRIAPVTQSVDVKGTPPYMSPEHFLDLKRTDE
                     RADIYSLGKILFEAVAGKMKQDQIPF"
     gene            1464..2477
                     /locus_tag="PLM79_00645"
     CDS             1464..2477
                     /locus_tag="PLM79_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOV84839.1"
                     /translation="MKWMVFSVVVLLSLGLVVPCWAGARSGIVTMEFDLSDHQSGEEM
                     RLWIPYPISDGEQFIGDIQVRGDYTSTGVYTDREHGNPILFAFWDKDARSRKLTFSFS
                     AERMEVIRRDFPAKEARWDPADYKTYLAPTRLGPTDGKVKELAYEITRGKKTVLDKAN
                     AIYEWTCKNMYRDPNTRGCGRGDVCSLLEKPGGKCTDISSVFVALTRASEVPAREIFG
                     IRLGKKPEEDITGYQHCWAEFYLPGYGWAPVDPADVRKAMLVENLKLEDPKTAEYKAY
                     FLGGIDAYRIKLSVGRDITLNPPQAGEPLNTFGYPFAQEGGRTIDWLDPASFRYRIVF
                     REH"
     gene            complement(2653..3108)
                     /locus_tag="PLM79_00650"
     CDS             complement(2653..3108)
                     /locus_tag="PLM79_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84840.1"
                     /translation="MILRTTMIFSLIMLLVAGSGVAAAQTGQEAGEQQQLATDQGTAG
                     QSATDQSNAGQGVRKGSGMKCCMGMMGMKHGEKDSDRGEMMSHDMDRGERDEDSGRSS
                     RMENLMIQNNTLMQSNMLLQQTLQQQQRQIDELRQHLDALEREMKEQRT"
     gene            complement(3241..5022)
                     /locus_tag="PLM79_00655"
     CDS             complement(3241..5022)
                     /locus_tag="PLM79_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV84841.1"
                     /translation="MADKAPSPKSPLAIGVFAGALFVTSILLVLTGWHIWDSYSDSKM
                     IQSKDFRLIELSWTVFHRDEVLVMSVRMAATTGKPEWEKRYREYAPQVSSAIAEAKAL
                     YSEEHISEAISQVDAINRRLTALEERAFDMVRRGDREGALDLLASDEYEKEMLICSEV
                     SVQLISTVRTHMESALGHQGRQAYRAVAWAAAALPVLLTTWFSALMMLKRYLNERNRA
                     EAALRRSEERYRMLVETMNEGLATQDENRIITYVNDTFCKMLGYAKEELMGKPLATVF
                     AESSRSVFEGEMARRMTGLRTTYEAELARKDGHRITAIISAAPLTDEHGSFKGAIGTV
                     TDITARKQAEESLRLYAARLERSNEELQEFTFVASHHLQEPLRKIRVFNSLFSTKYKN
                     TLDCQGRCYLERMDCSASRAQSLIHALLGYSRIAATPKASDAVSVRDAIEGALADLKD
                     PIEKTEARIEYEGDPCRIEADREQLRTLFRGIIDNAVKFRGKERPAVRIHSRITNGYC
                     ETTIEDKGIGFDEKYLNMIFRPFQRLHKEDEYRGIGIGLALSRKIAENHNGSITAKSI
                     PGEGSAFTVSLPVSRGANPSCLSCVPG"
     gene            complement(5266..5397)
                     /locus_tag="PLM79_00660"
     CDS             complement(5266..5397)
                     /locus_tag="PLM79_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84842.1"
                     /translation="MFKVSSLQKRLFLFLLFPVAFLLIAMGLLIFLITLPGSLSSPW"
     gene            complement(5420..7528)
                     /locus_tag="PLM79_00665"
     CDS             complement(5420..7528)
                     /locus_tag="PLM79_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOV84843.1"
                     /translation="MTEMETIEIPVAGLDCAECARHVKDAISDLPGVESVEVYLGSKK
                     ALVRHDPAVINLSSIRKALESAGYSVSDPATQPSLSPLSGFTRRILIFLGAVFGAVLF
                     IIVLGEWFGFFEKITQSVPFPVGLAIVIAGGWPLFKSVIRAALRLQVTSHTLMTMGLV
                     AALAIGEWATAAVIVFFMRMGDYAESFTTEGARRAVKDLAAMAPQTARIEREGSEVEL
                     PVSEVGIGETVIVRPGEKIPVDGEVISGQATIDQAAITGESMPVEVAAGSTVFAATIA
                     QLGSLRVRATRIGSDTTFGRVVKMVEEAEARRADVQRFADRFSAWYLPVIAGVALLTF
                     LIRQDPMATTAVLVVACSCSIALATPIAMLASIGASARRGLLVKGGKYLESLARADVV
                     LIDKTGTLTLGRPEITDVVPLNGLNSSQVLAFAASAERFSEHPLAEAVRAAAREKNLT
                     LHEPENFEALPGKGVQASVDGSLIAIGNRRMVPSASTVPVAFELEGQGKTLLFISRNG
                     ELAGVLAAADTLRPEVPAALKRIRDLGVSHIELLTGDNERTASALGEKLGVSYRASLL
                     PEDKIAVVKEYQAKGSTVVMIGDGVNDAPALAQADVGIAMGAAGADIAIEASHVALMR
                     EDWNLVPEAFETARKTMGVVKMNLAFTALYNLAGLTLAALGFLPPVLAAAAQSLPDLG
                     ILGNSARLLRQRSSSDSSKI"
     gene            complement(7573..7947)
                     /locus_tag="PLM79_00670"
     CDS             complement(7573..7947)
                     /locus_tag="PLM79_00670"
                     /inference="COORDINATES: protein motif:HMM:NF021378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOV84844.1"
                     /translation="MRANEQFKKKSLLWMAVAALLLFSAKESFAQGSNYPCWGMGPWM
                     MGGAMGWIGGIFMIFFWILVLVALVLFIRWLVGAGANRRVLPGAQLSQGPVESAADIL
                     KKRYARGEITREQFESMRRDVE"
     gene            complement(8033..8527)
                     /locus_tag="PLM79_00675"
     CDS             complement(8033..8527)
                     /locus_tag="PLM79_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84845.1"
                     /translation="MKRNLFILPVFVLIMLLGIAGVFAQQQAPGAGKDQAQGSGSQQG
                     GWYCPHCGHMQGMGSGMMGRGMMMNCPMMGQGGTMGHGGMMHGGTGQGQPGAPLTEDQ
                     AKTMVDNYLKSMNNPNLKMGKLTGPEGKDYYEAEILTKDGSLVDRIQVNKNTGWFRSA
                     YAQQ"
     gene            complement(8734..9819)
                     /locus_tag="PLM79_00680"
     CDS             complement(8734..9819)
                     /locus_tag="PLM79_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84846.1"
                     /translation="MGKSSSLCSRMPQAWPGFREQIFKRLVSEKRRVALIAAMIAVIT
                     LLHYNTRVSLHHYHILFRELYFVPIILAGFLYGVRGALTTSLSITALYIPFVLMTWEN
                     FSPDDFDTLIEIVLYNVMALSLGILSDRERMRQKHLRDSESLAAMGRAMSGVAHDMKG
                     PLVVIGGYSQLVQKDFQKEHPSYQKLDVVCRQVQRLENLTRDMLDFARPLELRLSEED
                     INTVVSGALSTVELEARKKGVRFRLELSGNMPPARIDSMRMERVLINLLMNAVQASPE
                     GETVTVETLRHGRNLVIDIIDCGCGIESGEREKIFEPFYTTKKEGTGLGLAIAGKIVE
                     AHGGSIKIVDNHARGATFRIVLPAGVY"
     gene            complement(10419..10952)
                     /locus_tag="PLM79_00685"
     CDS             complement(10419..10952)
                     /locus_tag="PLM79_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84847.1"
                     /translation="MLNGKGRVSTIIVMVLALLIGTASLSLAATAQWCPGPGGGGTGT
                     GWGCGQGWTGGTGIGWCNGMWGGNYNQRGTQFMAHGKIVAIDTAASSITVTMDGASPN
                     LLTKLGFTRADLPTDVTFQMKSTVKVWGCGWHNKQWGSGTVTLASLKAGDIVNLMGYF
                     DKTSGDPIVSRINVWFY"
     gene            complement(11774..12226)
                     /locus_tag="PLM79_00690"
     CDS             complement(11774..12226)
                     /locus_tag="PLM79_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV84848.1"
                     /translation="MAGKDRPLLLIVDDDIAHRLMLATLFEEWGYRVEEAADGLAALD
                     FIRSNPVDIILMDVRMPRMNGIEATKEIQRIDSDLPIIVMTAYSSLSTGMEALNAGAF
                     DYLTKPLDFDALRAVVDRARECGRRDGEDGKAGRKSCGFRDGLHSVAL"
     gene            complement(12186..13817)
                     /locus_tag="PLM79_00695"
     CDS             complement(12186..13817)
                     /locus_tag="PLM79_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84849.1"
                     /translation="MTGMMGMGWGHRQHQLLLEETAQQSDVLYIALVNPMGQVIAHSD
                     PTQVGKVLPLSFPSPGKTSHHFSEKEQKSFDVMRAYQPWFRQRGHRAGGSTDMCESWS
                     TQAQPQWSSSAQPQGELFLMVGLDPKPFEEASSQDIHQTILLFGIMFLVGAAGLVSLI
                     WAQHYRSARCSLQDVRAFASTIENQMPVGMMATDQEGRIERANEAARLILRQPEGITG
                     MIDDFPCFLPVANRLKKEERVVEQEIQCRVSETETAPLLVNAAHIRDGEGRKTGYVLL
                     FSDMTNIKQLEEQLRRSERLAGLGRLAAGVAHEIRNPLSSIKGFAKILAGRFKEDERS
                     RNIAEVMEQEVERLNRVVTELLDYARPTELQKKTLACKDLIKNSLRLIERDALQQGIR
                     VESTVHPEDLLVEVDPDRFTQVLLNLYLNALQAMDGGGTLKLAAFREDDQAVLKVSDS
                     GSGIASEHLPHIFDPYFTTKPKGVGLGLANVHKFVEAHGGEIEVESTQNRGTTFFIRV
                     PAAGEDSPATGLLAGKSAGGYEDGRERQAIASHSG"
     gene            14402..14653
                     /locus_tag="PLM79_00700"
     CDS             14402..14653
                     /locus_tag="PLM79_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84850.1"
                     /translation="MKKALSIMALAALIVTSGMGIAHARQTGRNYYGSQQGGYHCPWM
                     GQSGNTGRGGWYCGWRGNGQGRGWHMGPRSGGYTGPRGR"
     gene            15102..15524
                     /locus_tag="PLM79_00705"
     CDS             15102..15524
                     /locus_tag="PLM79_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84851.1"
                     /translation="MKKAMLIGSVLMLALTMSMPTLYAQQQGTQPQGQGWYCPWMGQG
                     GGMRHGGGMGCCMMGQGGAGQSGMMAPNLNQGQPLSKEQAKQMLDNYVGYTNNPNLKL
                     GEIADKGDLFEATIITKDGSLVDKIQIDKKTGWFRNVS"
     gene            15668..15955
                     /locus_tag="PLM79_00710"
     CDS             15668..15955
                     /locus_tag="PLM79_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84852.1"
                     /translation="MKKMMLIGLVLVMAFAFSAPAIYAQQQNTTPQTGGWYCPMMSQG
                     AAQGGWYCPMMHQGQAGKGGWYCPMMNRQASAGRGCCGMMNYNQAPPATKN"
     gene            16472..18361
                     /locus_tag="PLM79_00715"
     CDS             16472..18361
                     /locus_tag="PLM79_00715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOV84853.1"
                     /translation="MSNTSNKTFETGAVLDGKWVILEFIGKGGMGEVYRAHQINLNRD
                     VAIKVISREFVKSMEGDTEAMNLSLDRFRREVQTMAQVRHPNVLQIFDHGSVTVKKGD
                     EDIPIEYIAMEYIPGGTLRDTMSQEGFHPEEGRIREWLLSYFLPLLDGVWAMHEAGIV
                     HRDLKPENVLLDGKIPKIADFGLARSNRLKPFTQSMDMRGTPPYMSPEHFFDLKRTDE
                     RTDVYALGKILFEAVSGKMSPDQIPFKQARIPDAPTHFFQELDRIVQEATAEERDIRM
                     PSVEALKQALTEALKSVEKPSTLKSIQAPRGYHLKTSLLVAVLVALFGIAAGLGFLLL
                     HERSHAPADSRRASLPPLTFGEIFEGERTPGKEISPAESLRAKDGTTLRLVHGGKVTL
                     KGKGTGESERAIEVAPFYMDETKVTNYQYIEFLNQVLPRIEVKNGIVRGDGRIWLLLG
                     EVTKGCDPIGFKDGKFYVLESPHASCPVLRVTPYGAFAYTSFHGMRLPTEAEWLLALG
                     EDFKTPSKVPAAKGRSLDQCELPPSPSEQPGSENLPVCSPVGLFKANAHGIRGLNVGG
                     EWGIRGAMPSSEEKSQTAEFVALGGFLNDEEAGDGRPAAIRRYGWEAFEEVGFRCVVS
                     APSQT"
     gene            18379..19116
                     /locus_tag="PLM79_00720"
     CDS             18379..19116
                     /locus_tag="PLM79_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV84854.1"
                     /translation="MRIAIVSDIHGNLEALRKVLADIDRSNVERIISLGDNVGYGPEP
                     ERVLNLLRERNILSLMGNHEQGLIDSSHAPLFNPLAWQSLNITRKLLSQGSLDYIRDM
                     RVKLAEDQSLFVHGSPPDSLTRYFIAASLDEKRQIFHDMEEKMCFVGHSHALALVSFD
                     GKKVEHDLFPEGIIHLPRGRKYIVNAGSVGQPRDGDSRAKYVVWDSRKRRLEVHMVPY
                     DVGKTVRKILELGFPPHNAERLSGHVH"
     gene            complement(19174..20610)
                     /locus_tag="PLM79_00725"
     CDS             complement(19174..20610)
                     /locus_tag="PLM79_00725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV84855.1"
                     /translation="MAQGNFPLILVVDDDKAHRMMLTDFMDEWGYQAKEADDGAKALE
                     FIHNHPVDLVLMDMRMPGMDGIEATREIHRYNPAIPIIIMTAYSSIPTAVEALKSGAF
                     DYITKPIDFDALKLLMDRALEHTRLQEENEGLRRQLAQLQVSDIVGSSPAMRRLLEMI
                     ALVAPSEATVLITGESGTGKGLVARAIHAMSSRRDKPLVEVNCAAIPETLIESELFGH
                     EKGAFTGADRQRKGRFSMADKGTLFLDELGELPLSLQAKLLRVLQEGEIQRVGSDVII
                     PVDVRIIAATNRNIQKMVSEGGFREDLYYRLNVMPMEVPPLRERGEDIPLLAQHFLKR
                     IAEKNHKAVKGISPRAMDLLLRYSWPGNIRELENVMERAVILLQGEYVSERELPMALQ
                     ELVQMTVAVCADAKAPEDNRSDATLADRERQAIYEILEETGGNKSETARRLGITRRTL
                     KLKLKKYAEENASSQLLAGEGKDRHGSR"
     gene            complement(20658..22355)
                     /locus_tag="PLM79_00730"
     CDS             complement(20658..22355)
                     /locus_tag="PLM79_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV84856.1"
                     /translation="MDFGHLRPSRWTWFSPWMILGMVCILAGILLVLAIKNIHREKGF
                     MERALLSQANILMRSLEAGSRTGMMGMGWGPQQIQLLVEETAQQPDVLYMAMVDSQGE
                     VIAHSNPRLVGTRLQITAPQEGESLHRFANEGQRAFEVVRSYQPFFRQRGGGSWDMCT
                     MWQTPPATSGNVLILVGLDPKPFEDALRQDMQQTILLFGIMFVVGAAGFISLVWAQNF
                     RTARQRLEDVEAFTSTIVNQMPVGMVATSRDGRIQKANEAARAILGHDSDFIGCVEDY
                     PCFMPVVERLRRDETVVGQEIKCLVNEAESIPLLVNAAAIQDGEMNTTGHVFLFTDMT
                     NIRQLEEQLRRSERLASLGRLAAGIAHEIRNPLSSIKGFATLLAKRSGGDEKSQRISR
                     VMVEEVERLNRVVSELLDYARPTELHREPHSCRELIDRTLQLVDKDARHRRVEIESAI
                     EPESLHVDVDPDRFAQILLNLYLNALQAMEEGGTLRIEAFQQRERTLFIVSDSGAGIL
                     PEHIPNIFDPYFTTKSGGVGLGLANVHKLVEAHGGEIEVESSPGQGTSFIIRIPNTV"
     gene            22734..23324
                     /locus_tag="PLM79_00735"
     CDS             22734..23324
                     /locus_tag="PLM79_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOV84857.1"
                     /translation="MARKIFVLFLMILLLGGLDYATSIHRSAFDLAYRELYLLPVFLG
                     AWWFGRKGGLATSLIVSMVYLPCAILAQTFGVAFYVGNLVEVIVFNAVGAIFGTMRDL
                     ERRREKEKLEAVMAMAGCVGHELNNPLAIAMQAARLIQEEMGPESEYQGDLQTIVRNL
                     RRMRDLIKKITGIKRVELTHYAANCHIVDLEKSSAA"
     gene            23492..25090
                     /locus_tag="PLM79_00740"
     CDS             23492..25090
                     /locus_tag="PLM79_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV84858.1"
                     /translation="MRQVKLSAKLTGAFAVVAVITLIVGFIGWRGISQINEALTEVTD
                     NHLPCVVGLATINDALANIDKHERVVVYETNPELVKRELAELAEAWQEAEKGRKAFES
                     LSQSEDEAKLWKEFLTKWDGWKKLHEEVVGLIKKGDDASRKTAYELSIGKSGEAYRKS
                     EELIHELIKSNMEGVEEFEASSRAQSKLAISLSLAGMLIGTIFAFSFGAFLSHTITKP
                     IRQVIEGLSEATGQLAAASGQVASASQHLAEGASEQAASIEETSSSLEEMTSMTRQNA
                     ENSGEANQLMAEANRIVSQAGESMSRLTVSMGEISRASEETQKIIKTIDEIAFQTNLL
                     ALNAAVEAARAGEAGAGFAVVADEVRNLALRAAEAAKNTAGMIESTVSKIREGVELVE
                     STERDFSEVARSVTKSGELMAEISVASREQAQGIDQVNRAVSQMDKVTQQNAASAEQS
                     ASASEEMSAQALQMGEFVTQLAVLVGGATGGHGEGETRGRGEGKIRRDRERTVLLHVS
                     ASTPRRTTAQGAPSSRRLPPLIGE"
     gene            25301..27661
                     /locus_tag="PLM79_00745"
     CDS             25301..27661
                     /locus_tag="PLM79_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV84859.1"
                     /translation="MSRGIFRLKYKVALLVLSVSLACAGTIGLFSFLTAKHHVSEMMD
                     LEYMGSVNRVKQLIRDYLEHAGHEVRFLLHLSPVQGIIRARSNNGFDPVESLSLKQWK
                     RQLENIFEQDAHVDGRYMQISLLDASGVELVRVERNGAGVRTVPEGELQDTSAEPFFS
                     RAMKLERDGIYTSPFHLHRERGRVVMPPIPVIQFASPVFDREGNRCAVLLICFSAKDI
                     LNVKHMFGKKMDSGNIFISDQAGFYLYNSRYPSREWGGAQNLGSGANLAQDYTDEPAS
                     RILSGDEGVAFFDGRELFYSTLRISGELSLTIGLDAPREMVEAPLRQFRIFLVFVIAG
                     ILAMTWAFSAYFVRPILASIHALTAATARIALGDLQTEVTVSSNDEIRDLTEGFSSML
                     EALRKKTGRLTGLYELGVRKEKSAREVADRMVSFIASTTGSGMAFVERANGNGREVVS
                     CFKDGRIVRAGKFPLEGAACEEILREGKAFCCDQAQARFPLDAFLKEQHVNGYAAVPV
                     VSGNGKPIGTVAAARRRAVVPAGEDIELLHTLSRLIALEWEQEAHISEIKEASLDTIH
                     RLARAAEYRDKDTGAHLQRMSRYAAAIARRMGMDERMVESILYAAPMHDVGKIGVPDR
                     ILLKPGKLDAEEWEIMKQHTLLGELILEGSQNHYIKLAKIIAATHHERWDGTGYPMGL
                     KGEEIPLIGRITAVADVFDALTSRRPYKEPFPLENAFAIIKEGKGSHFDPKVVEAFFA
                     VREEVLTIRTAYRDEDENRQSTVISDKQGVIYKQGLPITSFLISAH"
     gene            27721..27924
                     /locus_tag="PLM79_00750"
     CDS             27721..27924
                     /locus_tag="PLM79_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84860.1"
                     /translation="MKKNDWKCLIDALLFVDVCSMAAIGLLLAFVIPSGKGQDASKFF
                     LGLHRHDWGGVSISTFRFSCWDC"
     gene            27909..28067
                     /locus_tag="PLM79_00755"
     CDS             27909..28067
                     /locus_tag="PLM79_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84861.1"
                     /translation="MLGLLILHIWFNWTWVVSSARSYFGEKWKKALWSLAGAWLGVLF
                     LGWLTVKL"
     gene            complement(28204..28989)
                     /locus_tag="PLM79_00760"
     CDS             complement(28204..28989)
                     /locus_tag="PLM79_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV84862.1"
                     /translation="MAEKAQQKTELSVVKPDDKSKAAAKPEAPISVITVWGASGVGKT
                     LFGITSPYKPVLMLDTEKSSKPYKISGLYDFERYECLTWMGPHGLQKRLGGIKAGEWG
                     TIVIDTGTQFCDWVAKEIFYKASPKKLEKQSMLVWGDVKNQVRHYIMDLMAKAQVVVI
                     TAGSRLQKDGETREARILEPVYALSDLFIELRRDPNQRIPFGLTMPPTSKSRIMALPP
                     RIPQATWEGILRYILEKPADWQNLDSEESIPEKVLYPGIDAEE"
     gene            complement(29083..30015)
                     /locus_tag="PLM79_00765"
     CDS             complement(29083..30015)
                     /locus_tag="PLM79_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84863.1"
                     /translation="MPWMKALCPDGQTKTFDECLACDHECFDLEVREALFERERNWQA
                     IEHTGDRISVTALLGCMRSTYLERKTDYAATPESKWYSLRGELIHRLVERPDFDNPYL
                     RRSEIRLYANLNGCKISGQLDNYKLRFLNMGILKDWKSVGDNALHYVIMEGAKEDHIW
                     QTNVYAWLARQNGYRVDRIEVAYLSLMAVVKTGQPATLSEFLVNPPSRSGKRRNMIGR
                     PRLVKEYPSGKKRWESRYMVPPVPLYNDETVIEFMTPRVKCLSEAFKAGIMPPMADEE
                     VRKWKCEGYCNVRSECEAEEEKRGRSWTIPEQAA"
     gene            complement(30114..31592)
                     /locus_tag="PLM79_00770"
     CDS             complement(30114..31592)
                     /locus_tag="PLM79_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV84864.1"
                     /translation="MKIIQLTAENVKRLRAVQITPNGNLVPITGKNAQGKSSVLDCIC
                     YALGGERAIPEQPIRSGTAKAEIKLDLGDLIVTRTFTHKGSYLKVQSKEGFEWKSPQK
                     MLDQLFGKFSFDPWGFIQISEAKQKEVLLSMVTIPLSVEKLQQISGITLQQNGNDPIA
                     TLNGAYKTLYDQRAEHNRSCKRIEAAIASIEIPKGMEETEAMSVSGLFEERRQLEEAN
                     RENAGKRAALQDLHQHKDDLEERKAGLEAQKEELIRRLQELDQTIEETAAAIMDTETL
                     IETAEAELASINDHDFSEVDARISKADEINRIAGEVKRRKTLEAELAEEQHGANECTD
                     RLESITAYKAELMAQTNFPVQGLDFCKGTISYNGVPLRQASAAEQLRVSMAIAMALNP
                     KLKVIRIEHGSLLDRDSWKIIEEMAREQDYQVWVEKVADAPGDGIYIYDGRIFSGNGE
                     ATAQPQPDSNFQQEEANAEEETSRHLAHQASTSLHGQAPIPF"
     gene            complement(31773..32648)
                     /locus_tag="PLM79_00775"
     CDS             complement(31773..32648)
                     /locus_tag="PLM79_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HOV84865.1"
                     /translation="MNENIFQQACLIQLSTSCWQGSRILDSSIMEQIGDSEWLRGRKY
                     LVDQETLHPIRCIVSRARKDLEKRALPFPIIGLTLVPKEQLDSVEECLKTHKADFFRA
                     ADKFVDRYEDARETARQRLGDLFSDLDYPVDIRNKFGFEWRYFTLETPGKYGILTPEI
                     YQREKEKFHAMMEETRELAMSALRQEFASHVNHIVERLTRSEDGEPKIFKNSMIEKIQ
                     AYLDSFDARNLFHDTDLETMVARAKQIMRGVNPQSIREDHQLKTHIAGEMSRIKQAID
                     EAVIDLPRRKIRFAA"
     gene            complement(32824..33027)
                     /locus_tag="PLM79_00780"
     CDS             complement(32824..33027)
                     /locus_tag="PLM79_00780"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOV84866.1"
                     /translation="MKEIVIDILDTGEVKIETKGFHGKSCVAESQFLKDLLGKETARQ
                     LTPAYYQREGEKQVVKKHLPLCG"
     gene            complement(33024..33227)
                     /locus_tag="PLM79_00785"
     CDS             complement(33024..33227)
                     /locus_tag="PLM79_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84867.1"
                     /translation="MPEPFEPPVQPAANLIPIRQAYFPPAFWSFWDQLMDRQKAAYIL
                     GWIAELENVIRRMEQLARKEENA"
     gene            complement(33303..33728)
                     /locus_tag="PLM79_00790"
     CDS             complement(33303..33728)
                     /locus_tag="PLM79_00790"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HOV84868.1"
                     /translation="MSHVAKIELEINDLDTLKAACERLGVEFVPDQKHYRWYNEHVGD
                     YPLPEGFTVDDLGRCDHAIRVPGASYEVGVVRRGSKYVLLWDFYSAGGLERKLGKGAG
                     RLKQAYAVERVRREARLKGYQVREQRAEHGIRLTLAGKR"
     gene            complement(33729..33926)
                     /locus_tag="PLM79_00795"
     CDS             complement(33729..33926)
                     /locus_tag="PLM79_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84869.1"
                     /translation="MKFIVFSLSDFHIHQRDATTRVRVRFVDARNMDKAKEFMRTYYP
                     GAWAIVPKKTFDKGIVHAEER"
     gene            complement(34080..35159)
                     /locus_tag="PLM79_00800"
     CDS             complement(34080..35159)
                     /locus_tag="PLM79_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV84870.1"
                     /translation="MEKIFHVLDLPLPSEADIFSMQQDLAKGVSLQDGNGSPVEIAAD
                     LPSARAAKGLTEFEAETAFALSLVREGRFNPKVITAVKAQMIRKSGLMEFWEPASISD
                     VGGLESLKTYIRNRARAFEPGNEHLPKLKAILLAGVPGTGKSLTSKATASILGWPLIR
                     LDIGALKESLVGESERRMRQATRVIDAFGESTIWIDECEKVFAGVRSSGETDAGTTAS
                     MFAHFLTWLQETRTPVLIMATANDISKLPTEFIRRFDAVFFVDLPTTEERREIIRIMN
                     RKYNSGIPEAYAEKLQGYTGSEIEQLAKDSLFDGLHEAFSAIVPISRTMREEVTALRE
                     WAKSRARIANTPEGQELQQRKIRAS"
     gene            35742..35951
                     /locus_tag="PLM79_00805"
     CDS             35742..35951
                     /locus_tag="PLM79_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84871.1"
                     /translation="MLKKAFDKASQLPQNLQDQIAQGLLEEIEWELKWDETLAGSEDL
                     LEKLANKAFQEFEAGKTVEKGFDEL"
     gene            35948..36217
                     /locus_tag="PLM79_00810"
     CDS             35948..36217
                     /locus_tag="PLM79_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84872.1"
                     /translation="MKSHLSSDFIRCFRQLPGRIQKHARRSYKLWKKDPNHPSLDFKQ
                     VSRRNPVFSVRVGIGWRALGLKRGEAVIWFWIGSYAEYDHILKHL"
     gene            complement(36258..38528)
                     /locus_tag="PLM79_00815"
     CDS             complement(36258..38528)
                     /locus_tag="PLM79_00815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV84873.1"
                     /translation="MTLKDFLEKYGGTLKEIASKRNRPIYDPTGEGAWEEAMRKRIES
                     LRRSPFPKQAEAVLALLKGYKKNGAKGLFLTAEMGTGKTIMGIAVSFLLCPPESRTLI
                     MCPGHLVRKWIREITDTVPHARVVNLNGPGLRELLDLRLSKPAGREFYVIGKERSKNH
                     FRRASGVMERRHLQPICPMCGALVDPVNPKTRKDTCRECFEPLWQAWGENGLRRFAKA
                     EFVKRYFKKGAIDLFIADEVHQYKSGDSAQGQAFADFASISKKVLCLTGTLMGGYATN
                     LFYLLWRTNANRMRDVTGSYKSVQRFAERYGVIEEIIKEPLSDNSNSIGGKRTVTVRE
                     RPGISPLIFTDLLLEHSVFMRLSDISSHLPPYTEHVISVEMAAEQREAYADFEDVLKG
                     AVRQALACGDRSLLGAMLQSLLAYPDGARRGEKVKHPHHLMPDGSPVIVAAAPSLEID
                     LLSKEEKLLEILKQEKTRGRKVLFYLEHTGTRDLIPDIVERIDRSGLSALVLRQNTTS
                     AENRETWVKTKLSEGSYDLLITNPRLVETGLDLIEFPTIIFFQCGYSTFTLRQASRRS
                     WRIGQTQPVEVYYLSYTKTMQEAALSLMGTKMQVALMVEGDLSDRGLTALAEGDSSML
                     VLLAKSLIGEEEVKPVADAWTELMKTGIHAESRIDAEESEKETVTLEKGDRKASISFE
                     RIVRGKVYVRKGYAIGYIDGNKFYFRDGKVTYRDNRVVGEYRRNGTGTINNKPFQLVS
                     SGTGREYMLVEFRATA"
     gene            complement(38620..39204)
                     /locus_tag="PLM79_00820"
     CDS             complement(38620..39204)
                     /locus_tag="PLM79_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84874.1"
                     /translation="MEEERYRLVDGTYTGGKVYAYTRGYILDAEGNILWMSLFGSSMA
                     LKTLAAQLFSGHGADVRKAEGWRHIVRIQKGSNPLRALTQVTADVASKIIFQPDVFTP
                     WKSVSSDQRRILVFGKTLEEAKTRLFRAVDRLVSTPLSPEWTEWLWDNLEELDTRFFL
                     PDLMQFGICQYMELPAGFEEMLLRDLRSGALALS"
     gene            complement(39218..40330)
                     /locus_tag="PLM79_00825"
     CDS             complement(39218..40330)
                     /locus_tag="PLM79_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HOV84875.1"
                     /translation="MIYSSFIIPHSSFQMGWGNSMARLASQSKLGYYATPLEVAQHVK
                     QSVAFEPGCRLLDPCCGEGDALALIAQGHAVETYGIELERERLSKAQQVLGHLLWGDA
                     LREINVSSGFDFIFLNPPYDFEEGEEQNQRIEYRFLTKYRRVLSPLGMIVIIIPLSAL
                     ESELLRSEIARFSALEVRTFPEGELFERFHQLLLLGWKKSVTGKQFEQNLSLLADIAE
                     MPPDELPTLLGTTREMAGKRCEVGKVHSASSLEFRSQRLDPDRVLDLIAGSTLWKDFF
                     AHVEPPSMNDIVPLAPMRQGHLAMLVAAGFCNGAELSDPEDATRKILIKGTVKRVSEV
                     SSSEETETHDVTRIIHTHRIRVRMLCVNEARIEEIE"
     gene            complement(40365..40832)
                     /gene="ssb"
                     /locus_tag="PLM79_00830"
     CDS             complement(40365..40832)
                     /gene="ssb"
                     /locus_tag="PLM79_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOV84876.1"
                     /translation="MMNIVAIAGHLGADPEIRYTQSGLAVANLNIAVNEHFRDESGEQ
                     QKRTHWFRASAFGRTAEIAGEYLHKGSKVGVSGQLVSREWQDQEGNNRRSVEIRVRQL
                     ELFGNGNGNGQNGSTGAGQKAGSQNQSARRSDAYEAPPDDLPPPGLPEDEIPF"
     gene            complement(41086..41319)
                     /locus_tag="PLM79_00835"
     CDS             complement(41086..41319)
                     /locus_tag="PLM79_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84877.1"
                     /translation="MKINEISVMVCKKVTRNFNSWSVSHSATAEIQEGENPMECMRAL
                     RAVLCEKVNEGLGENGRGKLIISQAFPRPHAFV"
     gene            complement(41439..41972)
                     /locus_tag="PLM79_00840"
     CDS             complement(41439..41972)
                     /locus_tag="PLM79_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84878.1"
                     /translation="MILPSWITRRAKAILSILALYISVTGLVTFSLFMLEESIQMATF
                     GTWPAQEARDWETVRFGCERIKEANRTLKIINYSFGWIQPLAFLGYRSYGESTDVYIR
                     GLEAKIFANAPELFDGETISFTFTPQSTARGDEGFIHKNGRITFISSNDYSKIGKKRL
                     KGTVAVEGEKITIREAQ"
     gene            complement(41969..42292)
                     /locus_tag="PLM79_00845"
     CDS             complement(41969..42292)
                     /locus_tag="PLM79_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84879.1"
                     /translation="MKKIAALILVFAFLMPVLAHAEDMILNTKVERVSSKINKDGNPY
                     VIIFVQEERTLSGIAYTAEAPVFAFGASKNEASQVKEGETMKAIVSKRDNGGDTTYTL
                     RKIIR"
     gene            complement(42436..42885)
                     /gene="rnhA"
                     /locus_tag="PLM79_00850"
     CDS             complement(42436..42885)
                     /gene="rnhA"
                     /locus_tag="PLM79_00850"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOV84880.1"
                     /translation="MKEVKIYTDGACSGNPGPGGYGAVLCYKDHKKEISGFQSKATNN
                     AMELTAAIEALRLLKEPCTVTILTDSRYLVDGITQYIRRWMENGWVTSDRSPVKNKEL
                     WRSLQKEAAKHQVNWQWVRSHNGHPENERADELARKAIEEGGKSNGQ"
     gene            complement(43048..43185)
                     /locus_tag="PLM79_00855"
     CDS             complement(43048..43185)
                     /locus_tag="PLM79_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84881.1"
                     /translation="MRAFLQHRFNVLHVYCRLSGILGPRLARSIATVWERTAAYRILY
                     A"
     gene            complement(43993..44319)
                     /locus_tag="PLM79_00860"
     CDS             complement(43993..44319)
                     /locus_tag="PLM79_00860"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOV84882.1"
                     /translation="MKRGDIVLAVAAGDYGKPRPALVVQSDLFNDTHASILVCLMTSD
                     IQDAPLFRITVEPSDKNGLQASSQIMVDKIMAIRKDKIRTRIGSLDDEVLLRVNRSLM
                     LFLGLA"
     gene            complement(44316..44534)
                     /locus_tag="PLM79_00865"
     CDS             complement(44316..44534)
                     /locus_tag="PLM79_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019684433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HOV84883.1"
                     /translation="MAKANAAREKMQRYRERLRSAGLRPVQVWVPDTSAPGFAEEARR
                     QSLLVSRSPSEEEALDFIEAAADTDGWK"
     gene            complement(44604..46577)
                     /locus_tag="PLM79_00870"
     CDS             complement(44604..46577)
                     /locus_tag="PLM79_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOV84884.1"
                     /translation="MQYAMERNKIKAGTRTRAGTPPEQRREAMAEERKKRTRRKKEDL
                     PSQTHPNFQELPVGRIRGNKWNPRSSGFDGQAFDELVASIRDKGVLQPILVRSIQEEA
                     HDFEILAGERRHRAMCLIASQDSARSTIPAIIRECGDDEAFEICLIENLQRQDLSEAE
                     EARAFVEYVDQKGADGVVTLAERLGVSPRYVRRRVEIMRLPQPLIWAWQKEILQYGHL
                     EQFLRIEGKKDRLMLLKQIIAAWAEDEGIPTVRELKGRIDDASAELSRALFDKQGCGM
                     CAKNSETQKNLFGIEDDHARCLDSRCYHAKQKEWLIENWQSHVKALDPETNTNGIRFS
                     DEVSHTDCEPFYYRNAAEKCLSCENYVTVLSPSGNIHRNRACCGRVECRKEVLYSRSP
                     TTGSTQSPSTGEKTLTGRDGTENQDGTPAKAWHGEYFRELFFKDRIPEKAKEYDPFHE
                     KMIQLSLFAIIASNSYIHRWFAERLGLPKRPKDTDYSFFLLCRDIFPAIEALDPGPAV
                     DLLKEAAIEVLLSNRTVMPATRRSVAEFLGIDLAAEWRITREYLEKKTKAEIVSIINR
                     FNILERPDAWKYASESCGLKLKSSIGSLKKDQMIGILLDSGTELAGVVPAEILHDSNG
                     NTAAAIPGISDSDSNGTSDEPGSFDFPEEEIAI"
     gene            complement(46987..47295)
                     /locus_tag="PLM79_00875"
     CDS             complement(46987..47295)
                     /locus_tag="PLM79_00875"
                     /inference="COORDINATES: protein motif:HMM:NF025577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HOV84885.1"
                     /translation="MGVYVVNKKRFEGEGIYIGRPSVLGNPFAIGRDGDRAEVIRKYR
                     VWLWEQVKARGRVFAELVRMKKMSECGDVYLICWCKPEACHGDVVKSCIDWMSAEGIE
                     "
     gene            complement(47506..47877)
                     /locus_tag="PLM79_00880"
     CDS             complement(47506..47877)
                     /locus_tag="PLM79_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84886.1"
                     /translation="MNDDDFDPIARADTGISKEHTHDEIEAMLDRDDAYHKFLEHQKD
                     IFILLHKVLDEIDQEDREKGGETTSILREKILRGRDLSDAELDSAVFVEACRLLFGKI
                     STREFLQRMTIISRLVDLETE"
     gene            48094..48294
                     /locus_tag="PLM79_00885"
     CDS             48094..48294
                     /locus_tag="PLM79_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84887.1"
                     /translation="MKDCSFMLRLMHLLFRIIESRALKASVIRFFISSWSKEPKRSFM
                     VIQKKPYSPWTSPESLKISTKT"
     gene            complement(48549..49565)
                     /locus_tag="PLM79_00890"
     CDS             complement(48549..49565)
                     /locus_tag="PLM79_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84888.1"
                     /translation="MQAISYENGIHRELKSGHYGPYWSLEIHAWHEAGDIWPSFCHVE
                     GESWQQPHPGKDEGNGIRLVSLDEAFSISPDYTPVFVSQSERDIYEHTPFEQSQPMLP
                     HERADTCPRHLRPEELMHQAHCTPREAVHFADLLSILEASEEIAAMFCRWSRMKGAQP
                     TLQYLYRLSLALAEAEAISPEDVLECHSHTEYSAPETMAYHKVALDSEEEAGWIESRP
                     YWFQKLIDTVQSCTEIEQLKQLCKEVYPHLHGDYMSVFLAFYNARKNYLESRAEKFMS
                     KPAKAFIRRIREARNSKQLASIGANLYKFQKGEIKGPEMKRVEWKFIWAAYNAAKDKL
                     TPKE"
     gene            complement(50136..50888)
                     /locus_tag="PLM79_00895"
     CDS             complement(50136..50888)
                     /locus_tag="PLM79_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOV84889.1"
                     /translation="MRTHRRRASTLDLIVSESVRFIKAHEPPEGYFVAFSGGKDSIVS
                     LELTRMAGVKHQSFYSCSGIEPPEVVRFIREHYPDVRFLYPQMSFFDGIRKKFPPLRT
                     KRWCCDYLRKYPARNIPLKHRIMGIRAEESNSRAERPWIERNRREGTVVYKPIFLWKE
                     WTIWEFIEEHDLPYPSLYDEGWDRVGCVVCPFICSSNMKLIERNRKRWPGMYRAFEHA
                     VREWFDTRKAQGVRFREETAGEYLQNWYRGIG"
     gene            complement(50885..51481)
                     /locus_tag="PLM79_00900"
     CDS             complement(50885..51481)
                     /locus_tag="PLM79_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV84890.1"
                     /translation="MGTPKYTWSEEQDQAIREAYKKHYFRGALEKLTRHPLFIGYPKA
                     QIQNRAIVIGVSRELKRWTDEEEAVIAKYAGEVPIIRIQRILKSRGYTRSFVAIKGKI
                     RSAHLRQKPDTYSNEEVAVAMRCSSEKVKRWISSGMLKAEKDNPDGSGYWRIRPADLA
                     RFIRTHVFELEACMPDIPWLVSLIDEFRSQTQGRIVQR"
     gene            complement(51697..52239)
                     /locus_tag="PLM79_00905"
     CDS             complement(51697..52239)
                     /locus_tag="PLM79_00905"
                     /inference="COORDINATES: protein motif:HMM:NF036900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84891.1"
                     /translation="MMDPLSPSNILQLLDSRGISIRVHEAALRCSPKHLLTSEIRDLI
                     ARHKPEIFSFLTEQEQEGARNWHSLLGSGKTDMDVTYLDEALFLFAAEKIRTASDEAM
                     WNRYAPFWEKRLPHQAMKALETLRQELASAPAQRPPEQILMQERSRSETPDESNHPHT
                     GPSTPCDTTPSASHSPPVLP"
     gene            complement(52561..55083)
                     /locus_tag="PLM79_00910"
     CDS             complement(52561..55083)
                     /locus_tag="PLM79_00910"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOV84892.1"
                     /translation="MSDRYQELISSIKAQADCRSIFKRFWPDKYRERGNSLCPFHDDR
                     EPSMQVTADLAHCHAEGISLDAIDLYERGVGCSKGEAIRLLASESGLDDPQEMDHSRG
                     RATGPGSEYFARRMQEAVKRGLPEEALLYLERRGISSATLQRLKEREIFGWDSKLRGI
                     AFPLFDWKQERILGIQIIPPDGNKKRFIKGTDSKRAFFRFGSGTDFMVFCEGIVDALS
                     VAEALPDAEAICLLGAGLINKLAEIDLRPTSILFLDNDEAGREATAKILMKFGPVFRL
                     VDWSLAPDGMKDVNDLLRAGHSRIIEEMIKTSKVPAKDEIERITAPYASRKSRKAVQA
                     ESPDEGNGKKQTQAQQLVLLAGDVELFHTADDVIYATFPMDEHKETWSVRSKSFRRWL
                     AHRFYLDFEKPPGNQALQDAFNILEAKGQFEGELQEVFMRIAASGDCIYIDLANTVWE
                     AVEISPSGWSIVSNPPVKFRRTKGMLPLAYPERGGSLHSLKGFLNLPDDAALKLMVGW
                     IIQAAHPTGPYPILNLEGEQGSGKSITARLLRSLIDPSTAPIRTIPREERDLLISATN
                     GWIIAFDNLSGVPLWLSDALCRLSTGGGYAVRELYSNDEEMIFNATRPLLLTGIDQIA
                     SRHDLADRSIILHLPPIPEERRIPEKEIIESFQAAHPKLLGALCDAVSVALKNLPHTR
                     LPAHPRMADFALWVSAAEPALPWSQGEFLNTYYGNRKETVELSLESDPVSSAIRELMA
                     SRNEWQGTSSELLDKLSEIVSEKIQKSQSWPKRGNTLSNRLKRSATFLRAVGIEVEFF
                     RLSGSGSRKIVLKANREKTTSDNVRRETESTGTPAPDDWEEL"
     gene            complement(55234..55584)
                     /locus_tag="PLM79_00915"
     CDS             complement(55234..55584)
                     /locus_tag="PLM79_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84893.1"
                     /translation="MEEQAPSSCKHFISCNANLCPLDQEIALHSWFIGEDVCKSRDQT
                     NDPIIRRQKQLNRLKPQKYIDKPLSADWLKATAPQKRILSPEEKAERIARLNRRKDST
                     PAAWTGGKQGLSPY"
     gene            complement(55584..56690)
                     /locus_tag="PLM79_00920"
     CDS             complement(55584..56690)
                     /locus_tag="PLM79_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOV84894.1"
                     /translation="MVAPTILIPGIKGTTLINSNSLSFDTIWSGLQCRFETVLDLELT
                     EDTRFETAPGVIIERGDVEDLAYKESVLILERKIKSPVFIFGYDWRKSCAENGKRLKA
                     FVEYLGEKLCISRFNFLTHSMGGMVFSCFIKEFKENFDAINHAVITVCPFLGSVGALI
                     GLLVGEGGSRFPFFNGNDEFRKIARSFPSVYELCPVYPNAVTLFGAPEQNSFDIFNPD
                     HWQSNIADLPIFRKRLEAFRRFRDPQNPAMFPLSELPPQVKERFLILVAEGEETKAWV
                     VVSPENPSGKVRNFFDFNQPAGDGDGTVPLESSTAFHDHILTLSIQSKWTDRATHAFF
                     LNDGRVQSIISRFLRDDTVHPKWWSDIGGTVRRV"
     gene            complement(56694..57041)
                     /locus_tag="PLM79_00925"
     CDS             complement(56694..57041)
                     /locus_tag="PLM79_00925"
                     /inference="COORDINATES: protein motif:HMM:NF027409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOV84895.1"
                     /translation="MNGDNFYSMNELKILIISALLAIFGGFAKLFMASKRLTIYQFIS
                     CCFVSGFTGVMASYLVRCMDLSPALQSFVIGMSGFAGPAALSAFTLLYEKKLGLRALA
                     AKNGNPEQKGEDA"
     gene            complement(57105..57899)
                     /locus_tag="PLM79_00930"
     CDS             complement(57105..57899)
                     /locus_tag="PLM79_00930"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOV84896.1"
                     /translation="MKKALLVGINDYPGKQNDLMGCVNDVHNMQDLLVSLFGFPPENI
                     TIMTDRDATTENILEALDLLVSSAKPADQIVFHFSGHGSQVSDKDGDEPDRCDEIICP
                     YDLDWQSKVIRDDDLSRIFKSIKPGVHAEVFLDCCHSGTGLRGAGGKSANYMRRRFLS
                     PPDELLPQSQEPGVMCLKAIRPAKIKVLWAACRSDQYAADALLDGRYGGAFTSYLCNC
                     IRQAKGNINRNEVLKLIRKGIRKGGFIQVPQLETVSKFKKARILAA"
     gene            complement(57932..58228)
                     /locus_tag="PLM79_00935"
     CDS             complement(57932..58228)
                     /locus_tag="PLM79_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOV84897.1"
                     /translation="MAGTGMITGIVSDSTSGAPIAGAVIRTDGTLSKVVTGSKGEYSI
                     VDTACTMNVHITHPGYIRKDAVVVIEENVYNKRRDFRLKPRTSMSQSLSEGLAE"
     gene            complement(58296..59717)
                     /locus_tag="PLM79_00940"
     CDS             complement(58296..59717)
                     /locus_tag="PLM79_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HOV84898.1"
                     /translation="MPPKAIIVAGGGPYEGNHLWEAVERLTEQAYRTLYYRGFTKSNI
                     RLLTFGTPPDFDKNVISDERYADPSCARIQQAITTWAAGATDVVIYLCTHGGNDSLLI
                     NGTVSPCQSLDAASLDSWLDALQQKISGKIIVVIDSCFSGSFVDNLVAPSGAANRIII
                     CGTGDSQFGYFINHGSISFSGFFWPRILKGDSLWTAFKKAKAAIGFTCTQTAVLNDNG
                     NMIGNEAGDGTICASTYVDAGSIQQLPASAQKVIFVAGAQASSPSGNDPTTAIIKHVA
                     GLAYHVLKSRGYSDANIKMLGPSGLPGYDSPATLANLQSAIQSWIPGAANALIYLVGH
                     GAAGSFKLNASENLTPATLKSYLDSLQASYEGKVGVVIDSPFAGSFLPALSPPPAGKT
                     RIVVTSTDAFNTANFACSGDLSFSQQFWAEVLDDDSLREAFLAAKNAVRYAYLTQIPV
                     LDDDGDGIGNEKIDGEAARNFMV"
     gene            complement(59763..60443)
                     /locus_tag="PLM79_00945"
     CDS             complement(59763..60443)
                     /locus_tag="PLM79_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOV84899.1"
                     /translation="MGKSRVIFTADDPELRHISISISFNVTEHESHEAKIYSIIDGDT
                     LRARLSALGVVEKVRLLGIDAPETGSDEHARQQCEYLGVNTDTLHILARISTIHLLWL
                     CPKGTRITLKTTGSPRDDKDRILAGLFIGDLCVNRLLVEHGYAMTYRGLSDWEDYQDL
                     EIQAKQARRGIWGRCEEGYYLASSKTYHRPGCSSARYATTRFQTREEAKASNLSPCSL
                     CLPDYTRD"
     gene            complement(60446..61303)
                     /locus_tag="PLM79_00950"
     CDS             complement(60446..61303)
                     /locus_tag="PLM79_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HOV84900.1"
                     /translation="MKDQYEQLRKLSDADLLARCIWGEGRGEKVEGKIAIAHVVLNRV
                     KAKSWYGRSISDVILKPFQFSCFNASDPNLPHILKLSSDCAELAFCKAIAELVIRGHL
                     KDDPTGGATHYHAAGCRPSWASKLEYLCQIGNHLFYKETSGRSIDEHDFLQHGAGPLP
                     RGENQHDERGKTMSDKDALSTIQQSGAILEQADDALQDLLDTLDSSAETETQNNTQST
                     TSSSVAQIAQANQSITNTVSAVSQCVSAAKQNDAKLTQSMTRSAESGAQTTEHSEGEP
                     GGSDSDEEA"
     gene            complement(61390..62295)
                     /locus_tag="PLM79_00955"
     CDS             complement(61390..62295)
                     /locus_tag="PLM79_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84901.1"
                     /translation="MAKKRKTSRKPGKDRRKPREDEAFLKEPGFKKQDISKKLIPLSV
                     LSGKDAEKAAERTIDNVLSAITHVLSEGDLSIIDFGRFKASKAKKTGYIKISFTPAGK
                     LKRAVEARLNAVPSPDPDSEGAPAPAVDPASAPEPPPQSQSEQASEETQPGVQPEASP
                     APAPEPSSETPAPAEEAPISEPEGVQETAESSETSGSDDAAIGTNVNSLPPEAPELKS
                     LDSISVENRTIVADGEEFGIAENLTVLDPGGNVMNFDDLVAALQHLTLVSKISIISKT
                     TYRVTAATISIINALQIHEYMGVVE"
     gene            complement(62355..62621)
                     /locus_tag="PLM79_00960"
     CDS             complement(62355..62621)
                     /locus_tag="PLM79_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV84902.1"
                     /translation="MASKDDLARAVAEKRGCPISEASSFIGTLIDVFQVTLKTEDVQL
                     VGLGSWKRATRKARRGRNPRTGETIMIPAKTVVKFKASKALTAQ"
     gene            63279..63839
                     /locus_tag="PLM79_00965"
     CDS             63279..63839
                     /locus_tag="PLM79_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84903.1"
                     /translation="MAGEEKENEVYFPATDCFREALNKRLDEEGHGAQARLAKAIERG
                     TKHLNDIVQGRRPASLDFQERVARFLKVPLDRMLAEGRELLKEKAKPFPYAEAAKKIK
                     TPEKRAEFICEKAVQDLELGESPLLPSQPIVREVARKALGDQWEEYIRGAMEDEDLFV
                     HTQDKIMLAIEELSKKIPSRKKRKRP"
     gene            64113..64289
                     /locus_tag="PLM79_00970"
     CDS             64113..64289
                     /locus_tag="PLM79_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84904.1"
                     /translation="MPIQNEAERILTEEACRWVSSPDGQRKLLEAIARSQKLIDSITA
                     AQEVDHKVLAEPIQ"
     gene            64480..66159
                     /locus_tag="PLM79_00975"
     CDS             64480..66159
                     /locus_tag="PLM79_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84905.1"
                     /translation="MEDIPRLANNRYEIHPYSIEKLNFLLAKKSGADPSVIRNKPHCD
                     RLKVYFNKIPTETIIVEEHYVDHHFLDDFTGYYVVCFKPYKRWCTRLHFFGNKFGKET
                     FEDILQKKIKGDELQEISDSYIGFIVVKPLPKTIFGKTCLKTLSEDPPGRKYPITRRY
                     RANLFGWNLEVKSLAFQEQDKVVGACSTSALWSVLQGTGVTFHHHIPTPVEITRSATQ
                     YVPPAPPETRHFPNSGLTVPHMAQAIKQVNLVPFYVSPRTADFQCVADYLKAIVYGYL
                     EGHIPILMTFHLVDYPEGEPPQLRKGIHAVAITGYLPDKHKAVPLRHKEGEPGCLLKA
                     SRISMLFVHDDQIGPFTRMEFSEEGREYILPRELAEGYDYSLSTSWRCAGGKPGKVRA
                     VPLALMIPLYCKIRIDYGTVLKAVVSLDGIIEMFRNTNRTTLSQQLEWDIYLTTLNDY
                     KTQLAQREDLDWNVRRQALIHGMPRFIWKATAYDGEKTVIDLLFDATDIEQACLLAHA
                     VPYSSNVYELLEYVSKDRKLSKMIKPTMAWKVLEWFSNLKKKNNSKGTLKK"
BASE COUNT        14504 a        18118 c        18466 g        15841 t
ORIGIN      
        1 gaaagggatt tgatcctgtt tcatcttacc ggcaacagcc tcaaagagga tcttcccgag
       61 agagtagata tcggcccgct catcggttct cttgaggtcg agaaaatgct cgggagacat
      121 gtaaggagga gtccctttga cgtccaccga ctgcgtcacg ggagcgatgc ggcaggagcg
      181 cgccaaaccg aaatcggcga gcttgggggc tgaaccgtcc agcaggacat tttccggttt
      241 gagatcgcga tggacgatcc ccatctcatg cagggcgcgg actccttcga gaaccgggat
      301 gaagtacctg gcgatccact ccctcgtgcg ctcttcgtca ggctcgaatc cctcggccga
      361 catggtggag cgaagcgttc ctcccggaat atactccatg gcgaggtact cgaccgccac
      421 gtcacgctcg cccttcttga tggaaacgct gccatggtcg tagatctgga gcacattggg
      481 atgacgaacc tgggccatga ccttgacttc ccttcggaac cggtcgaggc aggtttccgc
      541 ttcgtaggca ttgtcgccga tctcacggag ccatccttgg gagatgacct tgatggcgac
      601 gtcccggtcg aggttgagct ggtgtgcccg ataaacctcg cccattccac ccttgccgat
      661 gaagctgaga atcacccatt tcttctcgat caccgtgccg gtctcgaaag tgctgttcga
      721 agttacattc atcgcctgat gcgctccagt gctcttttct cggctttctt ttagaagtcc
      781 atcgaatgat aacacaatcc tctttgcggg gataggcgac tgctccgaag attcgagcaa
      841 ccacgaggaa tccccggcag ctataaagca atgcacgcag gggccgcttc atgtgggtct
      901 cggatgcacc gcatgaaatc catgtacgaa aagcaagtat cgtgccgtca gaaggagact
      961 ttacccggac ttgaaggatt tccggcataa gtctacctcc gtggggaaag gtgtcgattt
     1021 caagtatcct ctctatcgta gccatacccg agaggtcgtg tcatagcaag gactgaccac
     1081 gtgaccctcg gctgacgcgt tggagtccgc cccacaactg aaagcacaaa gccttctgtg
     1141 gcctcactgc cactatagtt tccgacttcc gtctgccgaa tcggtccgta cggccttccg
     1201 ccatcatctg tgactttttc tgcatcagct tttcgatggg agaggcagcc catgatagga
     1261 attacctatt tgatgcattg cttgacaaga catagaatcc atacgtttat ctgcttcctc
     1321 ggcgctagcc gcctcgagca tgccgccttt catgagcaag gttgagccgg cctcacctcg
     1381 aggactttag ggcttgcctg gggaggcgtg cacgattggc ataatcttag gattagtttt
     1441 acaagcagca tcaaggagaa tcgatgaagt ggatggtctt ttcagtcgtt gtgttgttgt
     1501 ctctgggact tgtggttccc tgttgggcag gggctcggtc cggtatcgtc actatggagt
     1561 tcgatctctc cgaccatcag agtggagagg aaatgagact ctggattccc taccctatat
     1621 cggatggaga gcaattcatc ggtgatatac aagtcagagg cgattacacc tcaactggag
     1681 tgtacacgga tcgcgagcat ggcaacccca tcctgtttgc cttctgggat aaggatgccc
     1741 gaagccgcaa gcttaccttc tccttttccg cagagagaat ggaggtcatc cggagggatt
     1801 tccccgccaa agaagctcga tgggaccccg ccgactacaa aacctacctt gctccaacac
     1861 gccttggccc cacggacggc aaagtgaaag agcttgccta cgagatcacc aggggaaaaa
     1921 agacggtgct ggataaggcc aacgccatct atgagtggac gtgcaagaac atgtaccgcg
     1981 atcccaatac acgtggttgc ggaaggggag atgtctgttc gctcctggag aagcccggcg
     2041 gcaaatgcac cgatatctcc tcggtctttg tcgccctgac ccgggcgtcc gaagtgcccg
     2101 caagggaaat attcggtatt cgcctcggga agaaacccga agaggacatt accggctacc
     2161 agcactgctg ggcggagttc taccttccag gatacggctg ggcaccggtt gacccggcgg
     2221 atgtcaggaa ggcaatgctc gttgagaacc tcaaactgga ggaccccaaa actgctgaat
     2281 acaaggcgta ctttttgggt gggattgatg catacaggat caagctctcc gtaggacgcg
     2341 acatcacttt gaatccgccg caggcaggag agccgctaaa cacctttggg tatccatttg
     2401 ctcaagaggg gggcagaact atcgactggc tcgatccggc atcttttagg tacaggattg
     2461 tctttagaga gcattagtcg gtggctgtat cgccatgttc tctactacag gagaaaggga
     2521 gcggtggtcc ggcctccctt tctcaccata agatgccggt gcataccgcg aagaatcacc
     2581 attgcgtcag tatcacctat ccgcagcagc attgtgatag catggaatgg agagggcctg
     2641 gaagacagag ccctatgtcc tctgctcttt catttccctc tccagtgcat cgaggtgttg
     2701 cctgagctca tcgatctgtc tttgctgctg ctgaagggtc tgctgcaaaa gcatattgct
     2761 ttgcatgagc gtgttattct gtatcatgag attctccatc cggctgctcc tgccggaatc
     2821 ttcatccctt tcgccacgat ccatgtcatg gctcatcatc tcgccacgat ccgagtcctt
     2881 ctctccatgt ttcattccca tcatgcccat gcagcacttc attcccgagc ctttccggac
     2941 accttggccg gcattgctct gatctgtggc gctctggccc gccgtgccct gatccgttgc
     3001 cagctgttgc tgttcgcctg cttcctgtcc tgtctgcgcc gctgctacgc cagagccggc
     3061 aacgagaagc attatcaggg aaaatatcat tgttgttcgt aggatcacgt tgctgtctcc
     3121 ttttgtcctc cagtttcggg aggcgctctt tcagacaagg agcgcccttt cacagtctac
     3181 ctggtctgtc tcctcaaaat actacttata aaagtaatca cgattcaatt agccgtctct
     3241 ttaaccaggg acgcaagaaa gacaggacgg gtttgctcca cgactcaccg gcaaagagac
     3301 agtgaaagct gatccctctc ccggtatgct ctttgccgtg atgctgccat tatgattttc
     3361 cgcaatcttt cggctgaggg caagaccaat gcctattccc ctgtattcgt cttccttgtg
     3421 aagccgttgg aagggtcgga atatcatatt gaggtatttc tcatcgaatc cgatcccttt
     3481 gtcctcgatg gtggtctcac agtacccgtt agtgatcctg ctgtggattc tgacggccgg
     3541 gcgctcctta ccgcggaatt taacggcatt gtcgatgatg cccctgaaga gcgtgcgcaa
     3601 ttgttccctg tccgcctcga tccggcacgg atcaccttcg tactcaatcc gagcctctgt
     3661 tttctcgatg ggatctttca aatctgcaag ggcgccttct attgcatccc gcacgctgac
     3721 ggcatcagac gccttcggtg ttgcggctat gcgggagtag ccaagcagag catgaatcaa
     3781 gctctgcgcc cgactcgccg aacagtccat ccgctcaagg tagcaacgcc cctggcagtc
     3841 gagggtgttc ttgtacttcg tgctgaacag gctgttgaac acccggatct tgcgaagcgg
     3901 ttcctgcaga tggtgtgagg ctacgaaggt aaactcctgc agctcctcgt tgctcctctc
     3961 aagccttgcc gcatagagtc tgagggactc ctcggcctgc ttacgggcgg ttatgtcggt
     4021 taccgtcccg atggcccctt tgaagctgcc gtgctcgtcc gtgagagggg cggcggatat
     4081 gatggcggtg atcctgtggc catcttttct tgctaactcg gcttcatagg ttgtgcgaag
     4141 gcccgtcatc cgcctggcca tttctccctc gaagacagac cggctggact cggcaaagac
     4201 tgtagcaaga ggctttccca tgagctcttc ttttgcatat cccagcattt tgcagaacgt
     4261 gtcgttcaca taggtgatga tgcggttttc atcctgagtc gccagccctt cgttcatggt
     4321 ctcgacaagc attctgtagc gctcttcgct cctgcgcagc gccgcctcgg cacgattgcg
     4381 ctcattgagg tagcgtttga gcatcatgag agccgaaaac caggtggtga gcaaaacggg
     4441 cagcgcggca gcagcccatg caacggcacg ataggcctgt ctgccttgat ggcccagtgc
     4501 ggattccatg tgggtcctca ctgtgcttat caattgcacg gagacctcgc tgcaaatgag
     4561 catctctttt tcatactcgt cgcttgcaag cagatcgagc gcaccctccc tgtctccccg
     4621 gcgcaccatg tcgaaggctc gctcctccag ggcggtaagt ctcctgttga tcgcatccac
     4681 ctgcgagatt gcctcgctga tatgctcttc ggagtagagt gccttggcct cggcgatggc
     4741 gctgctcacc tggggtgcat actcccggta tcttttctcc cattccggct ttcccgtagt
     4801 ggcagccatc cgaacggaca tgacgaggac ttcgtctcga tgaaaaaccg tccagctcag
     4861 ctcgataagc ctaaagtcct tcgactggat catctttgaa tcgctgtagg aatcccatat
     4921 gtgccatcct gtgagcacaa gcaggatgct cgtaacgaac agagcgcccg caaagacgcc
     4981 gatggccaaa gggctctttg gtgatggtgc cttatctgcc atatgctttt ccttaagggg
     5041 aaggttaacg gacattaaat tagaatcatt gcctggtcct gtgttcgatg gattttaacc
     5101 ggtttttgcg taatggtctg gcagtttcac aggcttacta ttgaagtgat cctgcaggcg
     5161 gtgtgcatac atggaagcga gacggagcat ccagaaacat gcgatgtacg accaaaccct
     5221 tcataagggt attgcaggaa ccctaatgcg gagcggattt caccactacc acggtgatga
     5281 gagactcccc ggcagggtga tgagaaagat cagcaagccc atggcaatga gaagaaacgc
     5341 caccggaaac agcagaaaca ggaacaggcg cttttgcaga ctgcttacct taaacatcgc
     5401 ttcacctgac acttttgatt caaatcttgg agctgtcgct tgatgatcgc tgccgaagca
     5461 gccgtgccga gttaccgagt atcccgaggt ctggaaggga ctgggccgct gcggcaagga
     5521 cgggcggcag aaacccaagt gctgccagtg tgagtcctgc aaggttgtag agggcggtga
     5581 aggcaagatt catcttgact acacccatgg tttttcgtgc ggtctcaaag gcttcaggca
     5641 ccaggttcca gtcctcccgc atcagagcga catgggaggc ctcgatggcg atatccgctc
     5701 ccgcagcccc catcgcaatg ccgacatcgg cctgtgcaag cgccggggca tcgtttacgc
     5761 catctcctat catgaccacg gtgctcccct ttgcctggta ctcttttacg actgcgatct
     5821 tgtcttccgg caaaagactc gcccggtagc tcacaccaag tttttcccca agcgctgagg
     5881 cggtgcgttc gttatctccg gtaagaagct cgatgtgact cactccaagg tctcgtattc
     5941 gtttgagggc agcggggact tcgggacgca gagtgtcggc tgcggcaagc actcctgcaa
     6001 gctctccatt gcgggagatg aaaagaagcg tctttccctg accttccagc tcgaaagcca
     6061 caggaacagt cgatgccgat ggaaccatgc gtcggtttcc aatggctatg aggcttccgt
     6121 ctacacttgc ctgcacaccc ttgccgggta gagcttcaaa gttctcgggc tcatggaggg
     6181 taagattttt ttcacgtgcc gccgcccgca cagcctctgc caggggatgc tccgagaacc
     6241 gttccgcaga tgccgcaaag gccaggactt gtgaggagtt gagcccattc agcggtacca
     6301 catccgttat ttcgggtctt cccagagtga gagtgcccgt tttatcgata agaacgacgt
     6361 ctgcgcgcgc aagggattcc aggtactttc ctccctttac aagaagccct cgccttgcgc
     6421 tggctccaat ggaggcaagc atcgcaatag gtgtggcaag ggctatggag cacgagcagg
     6481 cgacgacgag aacggcggtg gtggccatgg gatcttggcg gatgagaaag gtcaagagcg
     6541 caacacctgc aatgacggga aggtaccagg cgctgaacct gtctgcaaag cgctgcacgt
     6601 ccgcacggcg tgcttcggcc tcctcgacca tcttcacgac gcgcccgaag gtggtgtccg
     6661 acccgatgcg cgtagcccgg acccgaaggc ttccaagctg tgcgatggtt gcagcaaata
     6721 cggttgatcc cgctgcaacc tcaaccggca tggattctcc ggtaatggca gcctgatcga
     6781 tggtggcctg cccggagatg acctctccat cgacagggat cttctcaccg gggcgcacga
     6841 taacagtctc accgattcca acctctgaaa ccggcagctc cacctcgctc ccttcacgtt
     6901 cgatgcgggc agtctgcggg gccatggctg caagatcctt taccgctctt cttgcccctt
     6961 ccgtggtgaa gctctcggca tagtccccca ttcgcatgaa aaagacgata acggcagcgg
     7021 ttgcccactc tcctattgcg agcgcggcaa cgaggcccat ggtcatgagg gtgtgggatg
     7081 tcacttgaag ccttaaagcc gcgcgaatga cacttttaaa taacggccag cctcccgcaa
     7141 tcactatggc aagaccaacc gggaacggca cactttgcgt tatcttttca aagaacccga
     7201 accattcacc gagaacgatg atgaaaagaa ccgcgccgaa aaccgcaccg aggaagatga
     7261 ggatacggcg tgtaaagccg gataggggtg agagggaagg ctgtgtggcc gggtccgaaa
     7321 cggagtagcc tgcgctttca agggccttcc tgatagaaga gagattgatg acggccgggt
     7381 catggcgaac gagcgccttc ttcgagccga gatagacctc gaccgattcc acgccgggaa
     7441 gatcggagat ggcgtctttg acgtgtctgg cacattccgc acagtccagt ccggcaacag
     7501 ggatttcaat ggtttccatt tcagtcatat tttaggctcc atctagcttc gagcgcctgc
     7561 tccctggtgg gatcactcta catcgcgcct catcgattcg aactgctccc gggtaatctc
     7621 acccctggca tagcgcttct tcaagatatc ggctgcggat tcgaccgggc cttgagaaag
     7681 ctgagcccct ggtaagaccc ggcggttcgc tccggcacca acgagccagc ggatgaaaag
     7741 caccaaagca accagcacca gtatccagaa aaatatcatg aaaatccctc caatccatcc
     7801 catggcaccc cccatcatcc agggacccat tccccagcaa ggataattgg agccttgtgc
     7861 aaagctctcc tttgcggaga aaagcaaaag ggccgcaacg gccatccaaa gcagggactt
     7921 tttcttaaac tgctcattag ctctcatggg ttatcttctc cttctccaga taccaaagcc
     7981 tccccgtgga gaggaggctt tggtatcatc gccaactgca cggggttttc agctactgct
     8041 gcgcataggc agatcggaac cagcccgtgt tcttgttcac ctgaatcctg tccacgaggg
     8101 agccgtcctt tgtgaggatt tccgcctcat agtaatcctt gccctcgggt ccggtgagct
     8161 tgcccatctt gaggttcggg ttgttcatgg acttgaggta attgtccacc atggttttgg
     8221 cctgatcttc ggtgagaggg gctcccggct ggccctgtcc cgttccaccg tgcatcatgc
     8281 caccatgccc catcgttccg ccctgtccca tcatcgggca attcatcatc atgccacggc
     8341 ccatcattcc agatcccatg ccctgcatgt gaccacagtg gggacagtac cagcccccct
     8401 gctgagagcc tgagccctgt gcctggtcct tgccggctcc gggggcctgc tgctgggcaa
     8461 acacacctgc aatccccagg agcatgatca atacgaatac cggaagaatg aaaaggttcc
     8521 ttttcatggt cttctctcct tgctaaaggt tgagtgtcaa ctgccaactt cttctctgaa
     8581 tgtgcctcaa ggattgaggc accagtattt tcggctgtct caaccggaaa tacgttaatc
     8641 atggaataaa atgaaataaa tgctgccgcc gtttgcggcc agccggtatt gaaaatacct
     8701 gtaacaagcc gggaaccgtt cgtcgctgct ttctcagtac actccggcgg gtagcacgat
     8761 tctgaacgta gcgcctcttg cgtggttatc cacgattttg atagatccgc catgagcttc
     8821 aacgattttt cctgcaatag ccaatcccag tcctgtccct tccttctttg tagtatagaa
     8881 gggctcgaag atcttctctc tctcgccaga ttctatgccg caaccgcaat cgatgatatc
     8941 gatgacgaga ttcctcccgt gtcgaagtgt ttccactgta acggtctccc cttccggaga
     9001 ggcctgcacg gcattcatca gcaggttgat gagaacccgc tccattctca tgctgtcaat
     9061 tcgagcggga ggcatattcc cggagagctc cagcctgaac cgaaccccct ttttccgagc
     9121 ttcaagttcg acggtggaaa gggctccgga gacgaccgtg ttgatgtcct cttccgagag
     9181 acgaagctca agaggccgtg caaaatcgag catatccctt gtaagattct ccagccgttg
     9241 aacctgtcgg cagacaacat cgagcttctg ataactcggg tgctctttct gaaagtcttt
     9301 ttgcaccaat tgggaatagc cgccaatcac cacgagcggc cccttcatgt catgcgctac
     9361 acccgacatc gcccgtccca tggcagcaag gctttccgag tcccgaaggt gtttttgccg
     9421 catccgttcc ctgtcactca gtattccaag ggagagagcc atcacgttgt agagcacaat
     9481 ctcgatcagg gtatcgaaat catccgggga gaaattctcc caggtcatga ggacaaaagg
     9541 gatatagagc gcggtgatgc tgagagatgt agtgagagcc ccacgcacgc catagaggaa
     9601 ccccgccaga ataatgggaa caaagtagag ctcccggaag agaatatggt agtgatgcag
     9661 ggacacccgc gtgttgtagt gaaggagtgt gatcactgca atcatggcgg caatcagagc
     9721 tacccttctc ttttcgctga cgagtctttt aaagatctgc tctctgaacc ccggccacgc
     9781 ctgtggcatg cgcgagcaca gagaggacga ttttcccatt gtcttcctcc gtctgaatgt
     9841 gtcttcccga agaggccggc gatgtcttgc aggttcaccc tgcgatcttt gtcatcgcat
     9901 cagccccttc ccgaaaagaa aggagcatgc aaaggcatca atacccgtag tgaccgcagc
     9961 ccctccggtg cgcacagcct ccattattgt ggccgcaccc ctggtagccg tagccaccat
    10021 gatagccata acctccgtaa tggctgcatc cgccaagagc agcaaccagg gcaatcacca
    10081 atagaatctt tttgagcatt gcacacctcc gtagagaatc tctatacaag tccatttttc
    10141 cttcactgcc ccatggaatc ttgcaataca catgccatac cattatcata tcgattttct
    10201 tttattttcc ggcatgacgc aagttatttg cttcagccct ttgaagagaa cggatagttt
    10261 ttatccaggt gtggatagag tattaccagg cgatgctgga aacggagaaa aaaagccgaa
    10321 tctttcgatt cggcttcaca aggcggttgg cagtgtagga agattatgaa ttcaacatct
    10381 cttgggcaag acgctgaatt catgccgagc gctacaggct agtagaacca gacgttgata
    10441 cgcgacacaa tgggatcacc gcttgtctta tcaaaatagc ccatcaggtt tacgatgtcc
    10501 cctgccttga gagaagcgag agtcacggtg ccgctccccc attgcttatt gtgccatccg
    10561 caaccccaga ccttcactgt ggatttcatc tggaaagtca cgtccgttgg aagatccgca
    10621 cgggtgaatc caagcttggt cagaaggttt gggctggccc cgtccatagt aacggtaatg
    10681 ctggacgccg cagtatcgat agctacgatc ttcccgtgtg ccatgaactg ggttcctcgc
    10741 tggttataat tcccgcccca catgccgttg caccagccga taccagtgcc tccggtccag
    10801 ccttggccgc agccccatcc ggttcctgtg ccaccgccgc cagggcctgg gcaccattgt
    10861 gccgtcgcgg caagtgagag tgaagcagtt ccaatcagga gtgcaagtac cataacgatg
    10921 atggtggata ctctgccctt tccgttcaac atgactttag ctcctttaag cttaaatgat
    10981 aactgataag ccaaaccgcc ctgtttgata ctcttcttgc ttcgtgaggg ctgatgagcc
    11041 ttcgcgaagt ttccggaaag ctcgggtgtg acagttcccg agcgatttcg agatagacat
    11101 tgccaatcac aagcagactc agtgcttgca cgtttgatgc tcagttcggc gacagatccg
    11161 gctcttctcc gctgcataat acatctggca gcaaagaggg ctggacagcc agtcagtccg
    11221 aagatcaaat gtcgggagtt atcgagaggt gagttcttcg atctaaataa ggagaaccat
    11281 tgaatcaaca atggctagcg agaaatcttg tacgcagaat tgataaatcc aatacgtttc
    11341 aaagctcttg agggattgta gcggattatc gatggcaagg tctcccacca gcggtaggat
    11401 cgcaggtgaa gatcccggag cgggagcacc atcttgaagt acagcttcct tggtggaaac
    11461 cgtataatcg acacatggcc cacaaccatc tcctgagctt gtaagaggcg gttcgggtgc
    11521 catagcgctc ggacggacag gtcttagcgt atcttcggga caggcgctac aggcaaggtt
    11581 tgtctggtat tcaatctcaa tggtcccgtc ggccgcctgg cagagaacca cctgtccaat
    11641 cccacctatt gacgtccaaa gggagactgt aaggcaaagc caaacaaccg atggtctgac
    11701 acgcatgagc ttcactggta tagccttcct gtgtaatgac ggatgattcc caaaatgtct
    11761 cattgcggta tcttcataag gcaacagagt gcagcccgtc gcgaaaacca caggatttcc
    11821 ggcctgcctt tccgtcttcc ccatcccttc gcccacactc cctggcccgg tccaccaccg
    11881 cccttagtgc atcgaaatca agaggctttg taaggtagtc aaacgctccc gcattcaaag
    11941 cttccatgcc ggttgaaagc gatgagtagg ctgtcatgac gatgatcgga agatcggaat
    12001 cgatgcgctg gatctccttt gtggcctcga tcccgttcat cctgggcatc cgtacatcca
    12061 tgaggatgat atccacggga ttggatcgaa tgaaatccaa agccgcaagt ccatcggccg
    12121 cttcctcgac acggtagccc cactcctcga agagcgttgc aagcatgagc cggtgcgcta
    12181 tgtcatcatc cactatgaga agcaatggcc tgtctttccc tgccatcttc gtaacctcct
    12241 gcgctctttc ctgcgagaag ccctgttgcg ggcgaatctt cccctgccgc cggaacacga
    12301 ataaagaagg tggtccctcg gttctgtgtg ctttcgactt cgatttctcc cccgtgagcc
    12361 tcgacaaact tatggacatt ggcgagcccc aggcccaccc ccttgggctt ggtcgtaaag
    12421 taggggtcaa agatgtgggg gagatgttcc gatgcaatgc cggacccgga atccgaaacc
    12481 ttgaggactg cctgatcgtc ttccctgaaa gccgccagct tgagtgttcc accaccatcc
    12541 atggcctgta gtgcattgag gtagaggttt aagagcacct gggtaaaacg gtccgggtcc
    12601 acttccacca ggagatcctc gggatgaacg gttgactcca cgcgaattcc ttgctggaga
    12661 gcgtctcgct cgatgaggcg aagcgagttt ttaatgagat ctttgcaggc gagggtcttc
    12721 ttttgaagct ctgtgggccg cgcataatcg agaagctcag tgaccacccg gttgagccgc
    12781 tcgacctcct gctccatcac ttcggctatg tttcggctgc gctcatcttc tttgaacctt
    12841 cctgcaagga tcttggcaaa ccccttgatg gagctcaaag gatttcggat ctcatgagca
    12901 acacccgccg caaggcgccc aagtccggcc agccgctcac ttctcctgag ctgctcctcg
    12961 agctgcttga tgtttgtcat atcgctaaag agaagaacgt agccggtctt tctcccttct
    13021 ccatcccgga tatgggcggc attgacaagc agtggagccg tctcggtctc tgatacccgg
    13081 cattggattt cctgctcgac caccctctct tctttcttca aacggtttgc aaccggcaga
    13141 aagcagggga aatcatcgat cattcccgtg atgccttccg gctggcgcaa gatgagccgt
    13201 gctgcttcgt ttgccctctc gatccgcccc tcctggtctg tagccatcat gcctacaggc
    13261 atctggtttt cgatggttga ggcgaaagcg cgcacatcct gcagtgagca tctcgcgctg
    13321 cggtagtgct gtgcccagat gagggagacg agcccggcgg ctccaacgag gaacatgatt
    13381 ccaaaaagga gtatcgtctg gtgaatgtcc tgcgagcttg cctcttcaaa aggcttcgga
    13441 tccagcccga ccatgaggaa gagctctccc tggggctgag ccgaagaaga ccattggggc
    13501 tgagcctgag tggaccagct ctcgcacatg tccgttgatc cgcctgcgcg atgtccccgc
    13561 tgtctgaacc atggctggta ggctcgcatg acatcgaagg atttctgttc cttctcggag
    13621 aagtggtggg atgtctttcc tggcgaggga aaggagagcg ggaggacctt gccgacttga
    13681 gtggggtcgc tgtgcgcgat gacctgcccc atcgggttca ccagggcgat gtagagcaca
    13741 tcggattgct gggctgtttc ttcaaggaga agttgatgtt gccggtgccc ccagcccatt
    13801 cccatcattc cggtcatatt agctactccc tgtatgttcc atgtgttgct ttcgattgca
    13861 gaacaattcc tcgttccatc gcatagcaaa aagagaacca aattaaagtc tcaaagaata
    13921 cagcgagcga ctgatatgaa atctatccaa tgatttccga atcttaacca gcaccaaaaa
    13981 atatgtcctc ctctgaggca ccccacaagt ctttaaaaaa ctccgccatc cagctcaagc
    14041 tcagctcctt gagggcagat ggatggatag attctaccca cttgcgcaac ggggatggat
    14101 aattgctatc catcaacaaa ggacagccaa tctggggcca tatgcccaga acccaatttc
    14161 attaaaaaga atgtcttatt tcaataattt atcgccatag atcgaacttg gcatgctcta
    14221 tgctatgcat catggcgacg tgacaagatt tgctaaaagc caacgagtaa gaagaaataa
    14281 aatgaatcgg aagacaacag ggacgtgaac aaggaggtga attctcagga atggaatggc
    14341 tagagactta agagtaatta ggcaaccccc cgcactctcg tgaggcttta aggagaaaga
    14401 aatgaagaag gcactgagca tcatggcact ggcagctctc atcgtcacat ccggcatggg
    14461 cattgcccac gcccggcaaa ccggccgcaa ctactatggc tcccagcagg gcggctacca
    14521 ctgcccatgg atgggacaga gcggaaacac gggacgaggt ggatggtact gcggttggcg
    14581 tggcaacggg cagggccgtg ggtggcacat gggacctcgt agcggcggct ataccgggcc
    14641 gcgagggaga tagcccggat tcgagtatcg ggggatacca gccactatga gctggtgtcg
    14701 cctgggaaca gaagttttct tttacatgca ctccaaaaaa gaaatgaagg gaaagaagca
    14761 atgaggaagt tcttagtaat cggagccgta ctgactttga tgttcaccat gagccttcca
    14821 ggcgcttacg cccaaagccg tgggccgcaa cacaggcagg ggggatggca ctcgacatgg
    14881 aacgggcaga ggggaggcaa ctggaacaga ggatggacga atcagagacg cggcaactgg
    14941 caggggcggg gatggcacat gggacctcgc atgggtcagc gcggtcctgt agggcacggg
    15001 ccgcagcaca ggcctctcag gtaggccgaa cggaaagagc gggatatgga gtaatttttt
    15061 gccaggcaac ctggaacaat gatgagtaac ggaggaagac catgaaaaaa gcaatgttga
    15121 tcggttcggt attgatgctt gccctcacta tgagcatgcc caccctctac gcccagcagc
    15181 agggcactca gccgcagggc cagggatggt actgcccgtg gatgggacaa ggcggaggaa
    15241 tgcgccacgg cggtggaatg ggatgctgca tgatgggaca aggcggcgcg ggccaaagtg
    15301 gcatgatggc gcccaacctg aaccagggcc agcctctctc caaggagcag gcaaaacaga
    15361 tgctcgacaa ctacgtgggc tacaccaaca accccaacct gaaactgggg gagatcgccg
    15421 acaaaggcga tctcttcgag gcgacgatca taaccaagga tggctccctg gtcgataaaa
    15481 tccagataga caagaaaacc ggctggtttc gtaacgtatc ctagagtgga taggcgcggc
    15541 tgtccaaaca tccctccatg gttgaacagc aggagctcgg gcgcgtggag cctggctcct
    15601 gcaagaatct tagagtcagg tatccaacac agaaaatgtc atgcatgaag atgacaagga
    15661 gaaaacgatg aagaagatga tgctgatcgg cctggtactc gtgatggctt tcgctttcag
    15721 cgcgcccgcc atttacgctc aacagcaaaa cacaacccct cagaccggcg gatggtattg
    15781 ccccatgatg agccaggggg cggctcaggg cggatggtac tgtcccatga tgcatcaggg
    15841 gcaggcaggc aagggtggct ggtactgccc gatgatgaac cggcaagcca gtgcagggcg
    15901 cgggtgttgt ggcatgatga actacaatca ggcgcctcct gcaaccaaga actaagaggg
    15961 ccgactcttt ctgcaaaaca gcctggccgg agcactgacc aaaactcaca tgtcaaaagg
    16021 ggtagccgaa aaatgcggct acccctcacc aagaagatct cttcagcaaa gcacaccttg
    16081 ccttccgttc atcctgtcaa tcccgctccc gcaagagctc gatggtcgct tcagacagtc
    16141 tctcctttcg cgccatatat ctggtggata cattttatcc gctaaacctt ctcttattcg
    16201 ctccatgtat ccagaaataa aggctcacag cgctgacctc gcggaggaca ccacttgccc
    16261 ctatgccaaa agcatagcta ttcaaatatc ttagcgaata tctccagtgc ttggtatggt
    16321 ttgtgcttaa aggagttgaa cgaggataaa aagcattagg aaaactctaa cgcttcgggg
    16381 tatgaaaatg gaaatgcaga gcagtcatga agaagcgcat cccattcgag agttgacggg
    16441 aagagaagaa acaagtggag accaacctac gatgagcaat acgtcgaata agacttttga
    16501 gacaggtgcg gtgctcgacg gcaaatgggt catcctcgaa ttcataggaa aagggggcat
    16561 gggagaagtc taccgcgccc atcagatcaa cctcaaccga gacgtcgcca tcaaggtcat
    16621 ctccagggaa ttcgtcaaat ccatggaggg cgacaccgaa gccatgaatc tttccctcga
    16681 caggtttcgg cgtgaggtgc agacgatggc ccaggtccgc caccccaacg tgctccagat
    16741 cttcgaccat ggctccgtta cggtcaagaa gggtgacgag gacataccca tcgagtacat
    16801 cgccatggag tacatcccgg ggggcaccct gcgcgatacc atgtcccagg aaggcttcca
    16861 tccagaagag ggtcgcatcc gggagtggct cctgagctat tttctaccgc ttctcgacgg
    16921 cgtttgggcc atgcacgagg cagggatcgt ccaccgtgac ctcaagcctg aaaacgttct
    16981 ccttgatggc aagatcccaa agatcgcaga ctttggcctg gcccgctcaa accggctcaa
    17041 acctttcacc cagtccatgg acatgcgggg gacccctcca tacatgtccc ccgagcactt
    17101 ctttgacctc aagcgaaccg atgagcgaac ggacgtctat gccctgggga agattctctt
    17161 cgaggccgta tcgggtaaga tgagccctga ccagattccc ttcaagcagg cgagaatccc
    17221 ggatgctccc acgcactttt ttcaggagct cgaccgcatc gttcaagaag ccaccgcaga
    17281 ggaacgggac atacggatgc catccgtcga agcgctcaag caggccctga ccgaagcctt
    17341 gaagagcgtc gaaaaaccct ctactcttaa aagcattcag gctcctaggg gctatcacct
    17401 gaaaacatcg cttcttgttg cagtgctggt agcgctcttt ggtatcgccg ctggtctggg
    17461 attcctgctg ttgcatgagc gcagccatgc tcctgccgat tccaggaggg cctcactccc
    17521 tcctctgacc ttcggggaga tctttgaggg agagcgcacg cctggtaaag aaatctctcc
    17581 tgccgagagc cttcgcgcaa aggatggaac gacgcttcga ctggttcacg gggggaaggt
    17641 gaccttgaaa ggaaagggca ccggggagtc cgaaagggca attgaagtcg ccccattcta
    17701 catggacgag accaaggtga caaactacca gtatattgaa tttttaaacc aggtccttcc
    17761 aaggattgaa gtcaaaaacg gcatcgtgcg aggagacggc aggatctggc tcctccttgg
    17821 cgaggtgacc aaggggtgtg atcccatcgg cttcaaggac ggcaagttct acgtgcttga
    17881 atctccgcac gcatcatgcc ccgtgctgcg cgtcacccct tacggagctt ttgcctacac
    17941 cagttttcat ggaatgaggc ttcccaccga agcggagtgg ctcctggccc ttggtgagga
    18001 cttcaagacc ccatccaaag tccccgcggc caagggaaga tcattggacc agtgtgagtt
    18061 gccaccttcg ccttctgagc aacccgggtc ggagaatctc cccgtgtgct ccccggtggg
    18121 gcttttcaag gcaaacgcac atggaattcg agggctcaat gttggaggcg aatggggcat
    18181 tcgcggcgcc atgccgtctt cagaggaaaa gagccagacc gctgaatttg tagcccttgg
    18241 cggctttctg aatgatgagg aagccgggga tggccgtccg gctgctatca ggcgctatgg
    18301 ctgggaagcc ttcgaggagg tagggtttcg atgtgtcgtg agcgctccga gccaaacata
    18361 gcggcggatg cccggctcat gcgcatcgca atagtatcgg atatccacgg caacctcgaa
    18421 gcccttcgga aggttttggc cgatatcgac cgctcaaatg tggagcgcat catctctctt
    18481 ggagacaacg tcggctacgg ccccgagccc gaaagggtcc tgaacctcct gagggaaaga
    18541 aatattcttt cccttatggg caaccatgaa cagggcctta tcgattcctc ccatgctccc
    18601 ctgttcaatc cgcttgcgtg gcagtccctc aatatcacca ggaagctgct ctcacaaggg
    18661 agcctggatt acatcaggga catgcgcgta aaactggcgg aagaccaaag tctcttcgtc
    18721 catggctctc ctcccgattc gctcaccagg tatttcatcg cagcttcact ggatgaaaag
    18781 cggcagatct tccacgatat ggaggagaaa atgtgttttg taggccactc ccacgctctt
    18841 gccctggttt ccttcgatgg caagaaggtc gagcacgatc tttttcccga aggaatcatc
    18901 catcttccga gaggccggaa atatatcgtg aacgcaggaa gcgtaggcca accacgggac
    18961 ggcgacagtc gggcaaaata cgttgtttgg gacagtagga aaagacggtt ggaggtgcac
    19021 atggttccct acgatgtcgg caagacggta aggaaaattc tggaactggg gttccccccg
    19081 cacaacgctg agcgcctgtc agggcacgtc cattgacctg catgagcgtt ggtgagttct
    19141 tctccaaatt gtatttcttc ggcagcagtg atttcatctt gaaccgtgtc gatctttgcc
    19201 ctctccagcc aacaattgag aagaggcgtt ctcctcggcg tactttttca gcttcaattt
    19261 gagcgttcgc cgggtaatac ccaacctgcg ggccgtttca ctcttgttgc ctccggtttc
    19321 ctccaggatt tcatagatag cctgtctttc cctgtccgcc agtgtcgcat ccgaacggtt
    19381 gtcttcgggt gctttggcat cagcgcaaac cgctaccgtc atctgaacga gttcctgaag
    19441 ggccatgggc agctctcttt ccgatacgta ctctccctgg agtagtatga cggcgcgctc
    19501 cataacgttt tcaagctctc ggatgttgcc cggccaggag tatcgcaaga gaaggtccat
    19561 ggctcgcggg ctgataccct tgacggcttt atgattcttc tccgcaattc gttttaagaa
    19621 gtgctgggcc aggagaggaa tgtcctcccc tcgttccctt aagggcggaa cctccatggg
    19681 catgacgttc agccgatagt agaggtcctc tcgaaagcct ccctcggaaa ccatcttttg
    19741 gatgttgcga ttggtggccg ctatgatgcg cacatctacg ggaatgatga catcgcttcc
    19801 cacccgctgg atctcccctt cctgaaggac ccgaaggagc ttggcctgaa gcgatagagg
    19861 cagctccccg agctcgtcga gaaaaagcgt acccttatcc gccatggaaa agcgtccctt
    19921 gcgctgccgg tcggcacccg tgaaggctcc cttttcgtga ccaaagagtt ctgattcgat
    19981 caaggtttcg gggatagcgg cgcagttgac ctcaaccagg ggcttgtcgc gcctggaact
    20041 catggcgtgg attgctcttg ccacaaggcc cttgccggtt ccgctctcac ccgtgatgag
    20101 cacggtcgct tccgaggggg cgacgagagc gatcatctcc agaagacggc gcatagcggg
    20161 gctgctcccg acgatatcgg aaacctgcaa ctgggccagt tgcctgcgca gcccctcgtt
    20221 ttcctcctgg agccgggtat gctccagtgc ccggtccatc aaaagcttca gggcatcgaa
    20281 gtcgatgggt ttggtgatgt agtcgaaggc cccggacttc aaagcttcaa cggctgtcgg
    20341 gatggaggag taggctgtca tgatgatgat agggatggcg ggattgtagc ggtgaatctc
    20401 cctggttgct tctattccgt ccattcccgg cattcgcatg tccatgagga cgagatcgac
    20461 cggatggttg tgaatgaact ccaaggcttt agccccatcg tcagcctctt ttgcctgata
    20521 cccccactcg tccatgaaat cggtaagcat catccggtgg gccttatcat cgtctacaac
    20581 aagaatgagc gggaaattac cttgagccat ctatccttct ccttgatgaa tctttcaaag
    20641 tcttacttcc acaaggatca cacggtgtta gggatgcgga tgataaaact ggtaccctgc
    20701 ccgggagagc tttcaacctc gatctcgccg ccgtgagcct ctacgagctt gtggacattg
    20761 gcaagtccca gccctacgcc tccggacttg gtagtaaaat agggatcgaa aatattaggg
    20821 atgtgctctg ggagtatacc ggcgcctgaa tcggaaacga taaaaagtgt tctctctctt
    20881 tgctggaagg cctcgattct tagagttcct ccctcttcca tggcctggag tgcgttgagg
    20941 tagaggttta gaaggatctg ggcaaaacga tcggggtcta catcgacgtg gaggctttca
    21001 ggctcgatgg cagactctat ctcaactctg cggtgccgag catctttatc cactagctga
    21061 agcgtacggt cgatgagctc tctgcaggag tgaggctccc tgtgcagctc ggttggacgg
    21121 gcatagtcca aaagctcgct caccacccgg ttgagcctct cgacttcctc caccatgacg
    21181 cgagaaattc tctggctctt ttcatctcct ccggatcgtt tggcgagaag tgtggcaaat
    21241 cccttgatgg agctcagcgg atttcggatt tcgtgagcta tccctgccgc caagcgccct
    21301 agggatgcca gacgctcgct gcggcgaagc tgctcttcca gttgcctgat gttggtcatg
    21361 tccgtaaaaa ggaagacatg gcctgttgtg ttcatctccc cgtcctggat agcggctgca
    21421 ttgaccagca aagggatgct ttccgcttca ttgacgaggc acttgatctc ttggcccacg
    21481 acggtttcgt ctcttctgag ccgctctacg acaggcataa aacaggggta gtcttcaacg
    21541 catccgatga aatcactgtc atgacccagg atggcacggg cggcctcatt tgctttctgt
    21601 atccgcccat cgcggcttgt ggcgaccatc ccaacaggca tctggttgac aatggtggag
    21661 gtaaaagcct cgacatcctc gagcctttgg cgggcggtac ggaaattctg tgcccataca
    21721 agagagatga agcccgcagc gcctaccacg aacatgattc caaagagcag aatggtttgt
    21781 tgcatatcct ggcggagagc atcttcaaat ggtttcgggt cgagccccac caagatgaga
    21841 acatttccag aagtggcagg tggtgtctgc cacatagtgc acatgtccca cgagcccccg
    21901 ccccgctgcc tgaagaaggg ctggtaggag cgaacgacct cgaaagccct ctgaccttca
    21961 ttggcgaagc ggtgtaggct ctcgccctct tgaggggctg ttatctggag ccgggtgcct
    22021 accaggcggg gattactgtg ggctatgacc tctccttgtg aatctaccat tgccatatag
    22081 agcacatcgg gttgctgcgc cgtctcttcc acaaggagct gaatttgttg gggtccccat
    22141 cccattccca tcatgcccgt gcggcttccc gcttccaggg agcgcatgag gatgttggcc
    22201 tgcgatagaa gtgctctttc catgaaccct ttctctcggt ggatattctt gatcgcaaga
    22261 acgaggagga taccggccag gatgcacacc atgcccagga tcatccacgg agaaaaccac
    22321 gtccagcgcg aaggccgcaa gtgcccaaaa tccatttttc aatgctcctt aaaaagacga
    22381 taaaccagaa tgaaaaatcc ccaacccgtc tgccagttgc aagattggca ccaggtctca
    22441 atcactgccg tcctcatccc catgggtcaa ttttatccag gaatcgagcg ttcggctatt
    22501 gttcatggtt aattttgacc catgacaggt gcatcaaaac ctaccccaac catgacgacg
    22561 tgtcacggtt ccaatttttg cccttcaaga aaagagatga tctggcatgg ttcatgctcc
    22621 ttgctggaga tgatcgcaag gcagtgctca aacctgcatg gaaaagagcc ttgagggtag
    22681 aagggcctgc cttccctaaa aacattccac acgaaatgaa aggtgtgccg gatatggcca
    22741 ggaaaatctt cgttttgttt ctcatgattc tgcttttggg tggtctcgac tatgccacat
    22801 ccatccacag atcggctttc gatctcgcct atcgcgaact gtacctgctt cctgtctttc
    22861 ttggcgcctg gtggttcggg aggaagggcg ggctggccac atcgcttatt gtctccatgg
    22921 tttatctgcc ctgcgccatt ttggcccaaa ccttcggggt ggctttctac gtgggaaacc
    22981 tcgtggaagt gatcgtcttc aacgccgtgg gggcgatctt tggaaccatg agggacctcg
    23041 agcggaggcg cgaaaaagaa aagctcgaag ccgtcatggc catggccggg tgcgtcgggc
    23101 acgagctcaa caatccactg gccatcgcca tgcaggccgc ccgcttgatt caggaggaga
    23161 tggggccgga atcggaatac cagggcgatc tgcagaccat cgtgaggaat ctcaggagaa
    23221 tgcgagacct cataaagaag atcaccggaa tcaagcgggt cgaactgact cactacgccg
    23281 ccaactgcca catagtggat ctcgagaaat cctccgcggc ttagaggatt tgagggccgt
    23341 cctgttggcc tctgcgattg tggttggcgc cgtccgatat ctgtttgctt tcgacaaggg
    23401 atttcattag gctcagcagc tttaagcttt cggcaatgtg agaacgcgag cggaataaag
    23461 tgaatgtgga aaaacagagg gggaagcagc gatgagacaa gtgaaactaa gtgcaaagct
    23521 gacgggggca tttgccgttg tagccgtgat tacgctcatt gtgggattta tcggctggag
    23581 gggcatctct caaatcaatg aggctctaac ggaagtcacc gacaatcacc tgccctgcgt
    23641 cgtggggctg gccaccatca acgacgctct ggccaatatc gacaagcatg agcgcgtggt
    23701 cgtctatgaa acgaatccgg agctggtcaa gcgggagctg gccgagctgg ccgaagcgtg
    23761 gcaagaggcg gaaaaggggc gcaaggcgtt tgaatccctc tcccaatcag aagatgaggc
    23821 aaagctttgg aaagagttcc tcacgaagtg ggatgggtgg aaaaagctgc atgaggaggt
    23881 cgtcgggctg atcaaaaaag gagatgatgc atcccggaaa accgcctatg agctgagcat
    23941 cggcaagagt ggtgaggctt acaggaaaag cgaggagctc atccacgagc tcatcaaaag
    24001 caacatggag ggtgtcgaag agtttgaagc ctcatccaga gcacaatcaa aactggccat
    24061 aagcctctcc ctggccggca tgctcatcgg taccattttt gccttctcgt tcggcgcctt
    24121 tttgagccac accatcacaa agcccataag gcaggtgatc gaagggcttt ccgaggcaac
    24181 aggacagttg gccgccgcct ccggccaggt tgcctcggca agccagcacc tggccgaggg
    24241 ggcatccgag caggcggcat ccatcgagga gacctcctca tccctcgagg agatgacctc
    24301 catgacccgg cagaacgccg agaactcggg tgaggccaac cagctcatgg cggaagccaa
    24361 ccggatcgtg agccaggcag gtgagtccat gagccgcctg accgtttcca tgggggagat
    24421 ctccagggca agcgaggaga cgcaaaagat cataaagacc atcgatgaga tcgccttcca
    24481 gaccaattta cttgctctca acgccgcagt cgaggcagcc agggcgggtg aggccggagc
    24541 ggggtttgcg gttgtggctg acgaggtgag aaaccttgcc ctgagggcgg ccgaggcagc
    24601 aaaaaacacg gccggcatga tcgagagcac cgtctccaag atccgggaag gggtcgagct
    24661 cgtggagagc accgagaggg atttttccga ggtggcgcga agcgtcacca aatcgggaga
    24721 gctcatggcc gaaatcagcg ttgcatctcg tgagcaggcc cagggaatcg accaggtcaa
    24781 ccgggccgtt tcgcaaatgg ataaggtcac ccagcagaat gcggcaagcg ccgagcagtc
    24841 cgcatcggcc tccgaggaga tgagcgctca ggctttgcag atgggagagt ttgtcactca
    24901 actggctgtc ctggtcggag gagcaacagg aggacatgga gagggggaga cacggggacg
    24961 cggtgagggg aagattcgca gagatcggga gcgcaccgtc cttctccatg tttccgcatc
    25021 cacgccccgc cgcaccaccg ctcagggagc accttcttcc cggcggctcc ctccactgat
    25081 cggtgaatag ggtggtcggc ggataggggg gattcgggtt gcgagtcata gtcaaagtct
    25141 ggcaacttat ggaaatgagt tagcaatacg aaaaattatc ggcgcgccga taattttttt
    25201 ctgtccgata agacgactca aatcgtccaa atgacagagc agaactaacc cacaactcga
    25261 tttaccgcgt ttccatgtct tctctgggag ggggaattgc atgtcacggg ggattttcag
    25321 gctcaagtac aaggtcgccc tgctggtgct gtcggtgagc cttgcctgtg cggggaccat
    25381 aggccttttc tcgtttctta ccgcaaagca ccatgtgagc gagatgatgg atcttgagta
    25441 catggggtcg gtaaaccggg taaagcagct catccgcgac tacctggagc acgccgggca
    25501 cgaagtccgg tttttgctcc atctctcccc cgtccagggt atcatccggg ccaggagcaa
    25561 caacggcttt gaccccgtgg aaagcctaag cctgaagcag tggaaaaggc aactggaaaa
    25621 catcttcgag caggatgccc acgtcgatgg ccgctacatg cagatcagtc tgctcgatgc
    25681 ctcgggtgtg gagctcgtgc gggtcgagcg aaatggcgcc ggcgtgcgca cggttccgga
    25741 aggcgagctt caggatacct cggccgagcc ctttttttcc agagccatga agctcgagcg
    25801 agacgggata tacacttccc cgttccatct ccatcgggag cggggccggg tggtgatgcc
    25861 gccaataccg gtcatccagt tcgcctcccc ggtattcgat agggagggaa accgctgtgc
    25921 cgtgctcctc atctgttttt cagccaaaga tatcctgaac gtaaagcaca tgttcggcaa
    25981 gaaaatggat agcgggaata tcttcatctc ggaccaggcc ggcttctacc tctacaacag
    26041 ccgctaccct tccagggagt ggggcggtgc gcaaaacctg ggctccgggg ctaatcttgc
    26101 gcaagattat acggatgagc cggcatcccg catcctttcg ggagatgagg gggtggcctt
    26161 cttcgacggg cgggagctct tttattcaac gctcaggatc tcgggcgagc tctcgctcac
    26221 catcggcctc gacgctccca gagagatggt cgaagccccg ttgcgccaat ttcgcatctt
    26281 tctcgtcttc gtgatcgcgg gaattctcgc catgacctgg gccttttcgg cctatttcgt
    26341 gcggcctatc ctggcctcta tccatgccct caccgccgca accgcaagaa tcgccctcgg
    26401 ggatctccaa accgaagtca ccgtatcgag caacgatgag atccgggatc tgaccgaggg
    26461 tttttcttcc atgctcgagg cattgaggaa aaagaccggg cgcctgaccg gcctctacga
    26521 gctcggagtg cgcaaggaaa agagcgcccg tgaggttgcc gaccggatgg tttcattcat
    26581 tgcctcgaca acgggcagcg gaatggcctt tgtggaaaga gcgaacggga atggaagaga
    26641 ggtggtctcc tgcttcaaag acggccggat tgtgcgcgca ggaaagttcc ccctggaggg
    26701 agcggcctgc gaggagatcc tgcgggaagg aaaagccttt tgctgtgatc aggcccaggc
    26761 aagatttccc ctggatgcct tcctgaaaga gcagcacgtg aacgggtatg cggctgttcc
    26821 cgtcgtttcg ggaaatggaa aacccatcgg tacagtagcg gcagcccgaa ggagagcggt
    26881 cgtgccggca ggagaggata tcgagctgct ccacaccctg agccgcctca tcgccctgga
    26941 gtgggagcag gaggcccaca tcagcgaaat aaaggaggca tccctcgata ccatccacag
    27001 gctcgcgcgg gcggccgagt accgcgacaa ggatacggga gcccacctgc agcgcatgag
    27061 ccgctatgcg gccgccatag cccgcaggat gggcatggat gagaggatgg tcgagtccat
    27121 cctctatgcc gctcccatgc acgatgtcgg aaaaataggc gttccggaca ggattctcct
    27181 caagcccgga aagctcgacg cggaagaatg ggagatcatg aagcagcaca ccctccttgg
    27241 agagctcata ctcgaaggat cccaaaatca ctacatcaag ctggccaaga tcatcgcggc
    27301 cacgcatcat gaaagatggg acggaacagg gtatcccatg ggactcaaag gggaggaaat
    27361 ccctcttatc gggcgcatca ccgccgttgc cgacgtcttc gacgccctca cctccaggag
    27421 accttacaag gagccttttc cgctggagaa tgcctttgcc atcatcaagg aaggcaaggg
    27481 aagccatttc gatcccaaag tggtggaggc cttttttgcc gtaagagagg aggtcctcac
    27541 catcaggaca gcctaccggg atgaggatga gaacaggcaa agcacagtga tttcagataa
    27601 gcaaggagtg atttacaagc aggggctacc gatcaccagc tttcttatct ctgctcactg
    27661 aacacgcttt cggaccggtt gtgtgctttt gaaagctttt ctttcgcagg aggaaaaccc
    27721 gtgaagaaaa atgattggaa atgccttatc gacgccctgc tctttgtaga cgtgtgctcc
    27781 atggccgcca tcggattgct cctggctttt gtcatccctt cgggcaaagg gcaggatgcc
    27841 tccaagtttt tcctgggcct gcaccggcat gattgggggg gggtatccat ctctaccttt
    27901 cgcttctcat gctgggattg ctgatcctcc atatctggtt caactggacc tgggtggtca
    27961 gttccgccag gagctatttt ggagaaaagt ggaaaaaggc cctctggagc ctggccggtg
    28021 cgtggctggg tgtccttttt ctgggctggc tgaccgtaaa gctataagcg gcggatagat
    28081 ccgcttccag gggctcaagg tgaggtaccc ccctggattc tccatgcgat ttcccatgag
    28141 atcgaaacgg cttggcaaag gcatgacttg aagtcattgc tgccgatcac ggttccttgc
    28201 cggctattcc tcggcatcga tgccggggta caggaccttc tccgggatgg attcctcgct
    28261 atcgagattc tgccagtcgg cgggtttttc caggatgtag cgcaagattc cctcccatgt
    28321 ggcctgcgga atgcggggtg gcagggccat gatgcgcgac ttggatgtag gcggcatggt
    28381 aagtccgaag gggatacgct gattgggatc gcgacggagc tcgatgaaaa gatcggatag
    28441 ggcatagacg ggctcgagga ttcgggcctc gcgggtttcc ccgtcctttt ggagccttga
    28501 gcctgccgtg atcaccacaa cctgcgcctt agccatgaga tccatgatat agtgcctcac
    28561 ttggtttttt acatcacccc agaccagcat ggactgcttt tcaagctttt tgggactggc
    28621 cttgtagaag atctctttgg ccacccagtc gcagaactgt gtcccggtgt cgatcacgat
    28681 ggtgccccac tctccggctt ttatgccgcc aagccgtttt tgcaggccat ggggccccat
    28741 ccaggtaagg cactcgtaac gctcgaaatc gtagaggccg gagatcttgt agggcttgga
    28801 gctcttttcg gtatcgagca tgagcacggg cttgtacggg gatgttatgc caaagagagt
    28861 ctttccaacg cccgaagcgc cccagacggt gatgacgctg atgggagctt cgggctttgc
    28921 agcggccttc gacttatcgt cgggctttac gacggaaagc tccgtttttt gctgcgcttt
    28981 ttctgccatg agggttctcc tttgctcggg ttgcgagctg cgcgttgcgg gttgcaggtt
    29041 tgaaacggca gtatggtttc ggccgacatt cttcgagggt cttcatgccg cctgctcagg
    29101 gatggtccag gacctcccgc gcttttcctc ctcagcctcg cattcgcttc tgacgttgca
    29161 gtagccttcg cacttccact tccggacttc ctcatcggcc atggggggca tgatgcctgc
    29221 tttgaaggct tcggagaggc atttcaccct gggagtcatg aactcgatca ccgtctcatc
    29281 gttatagaga ggaacgggag ggaccatgta gcgggactcc cagcgcttct tgcctgatgg
    29341 atattctttg acgagccggg ggcggccgat catgttccgg cgcttgccgc tcctggaggg
    29401 cgggtttacc aggaactcgc tcagggttgc cggctggccg gttttgacca cggccatgag
    29461 ggagaggtag gcgacttcga ttcggtccac acggtatccg ttttgccggg cgagccatgc
    29521 atacacgtta gtttgccaga tatggtcctc ttttgctccc tccatgatga cgtagtgcag
    29581 cgcgttgtcg cccacgctct tccaatcctt caagatcccc atgttgagga agcggagctt
    29641 gtagttgtcg agctgcccgg agatcttgca gccgttgagg ttggcgtaga gccggatctc
    29701 ggagcggcga aggtaggggt tgtcgaaatc gggacgttcg accagtcggt ggatgagctc
    29761 gccgcggagg ctataccact tgctctcggg agtggccgcg tagtcggttt tgcgctcgag
    29821 ataggttgag cgcatgcacc cgagaagtgc ggtcacgctg atccggtcac cggtgtgttc
    29881 aattgcctgc cagttgcgct cccgctcaaa gagggcttct cgcacctcga gatcgaagca
    29941 ttcgtggtcg caggcaaggc attcatcgaa ggtcttggtc tgcccgtccg gacagagagc
    30001 tttcatccag ggcatggcgc atctccttaa tcaaacggtg aatcagtgag tcggtgaatg
    30061 ggtgaatcga gaggtgggct gctcgattca ccaccggcag actggaggca gattcagaat
    30121 ggaatggggg cttgtccgtg gagggaagta gaagcctggt gtgctagatg ccgggatgtt
    30181 tcttcctctg cgtttgcttc ctcctgctga aagtttgaat ccggctgcgg ttgagcggtt
    30241 gcttccccgt tgccgctgaa gatccttcca tcatagatgt agatgccgtc gcccggtgcg
    30301 tcggctacct tctcgaccca tacctggtag tcctgctccc gtgccatctc ctctatgatc
    30361 ttccaggaat cccggtccag gagcgagcca tgctcgatgc gaatcacctt gagcttcggg
    30421 ttgagagcca tcgcgatggc catgctcacc cggagctgct cggccgcact cgcctggcgc
    30481 agggggactc cgttgtagct gatcgttccc ttgcaaaagt cgagtccctg gacggggaag
    30541 ttcgtttggg ccatgagctc tgctttgtag gcggtaatgg attcgagcct gtcagtgcat
    30601 tcattggcac catgctgctc ctccgcgagc tccgcctcga gcgtcttcct ccttttcacc
    30661 tcgccagcaa tccggttgat ctcatctgct ttagagatcc tggcatcaac ctcggagaag
    30721 tcgtgatcgt tgatggaggc gagctcagct tcagctgtct cgatgagcgt ctcggtgtcc
    30781 atgatggccg ccgccgtttc ctcgatggtc tggtcaagct cctggaggcg tctgatcagc
    30841 tcttcctttt gagcctcaag gccagccttc ctttcctcca ggtcatcctt gtgctggtgc
    30901 aggtcctgca gtgcggctct tttgccggca ttctcccggt tggcttcctc aagctgcctg
    30961 cgttcctcga aaaggccgct cacgctcatg gcctcggttt cttccatccc cttcgggatt
    31021 tcgatgcttg cgatggcggc ttcgatcctc ttgcagctgc ggttgtgctc ggcgcgctga
    31081 tcatagaggg ttttgtaggc accgttcagg gtcgcaattg ggtcattgcc gttttgctgc
    31141 agggtgatcc ctgaaatctg ctggagcttt tccacggaga gcgggatggt gaccatggaa
    31201 aggagcactt ccttctgttt ggcttcgctg atctggatga agccccaagg gtcgaaggag
    31261 aatttcccga agagctgatc gagcatcttc tgaggcgatt tccactcgaa accttccttg
    31321 ctctgcactt tcaggtagga gcctttgtgg gtgaaggtgc gagttacaat gagatcaccc
    31381 aaatcgagct ttatctctgc ttttgccgtt ccgctccgga tgggctgctc ggggatggct
    31441 ctctcccctc ccagagcata gcagatgcaa tcgaggaccg agctcttgcc ctgggcgttc
    31501 tttcctgtga tgggtacgag atttccgttc ggggtgattt ggaccgccct caggcgcttc
    31561 acgttttctg ccgtaagctg gatgattttc ataaatacct ccttcatctg tgatagttgc
    31621 ttgcaggtgg tggctggcgg gaaccgggct gctggggacg caaggctcgg cgccccctta
    31681 tcagttcact caccaacaga cgaagaatcc attcggtcgg gatgcgaagg tggatgaaga
    31741 aggtatcgtg atcagggtga aatgccttgc cttcaggcgg caaagcggat ctttcttcgg
    31801 ggaagatcga ttacggcttc atcgattgcc tgcttgattc tgctcatctc acctgcgatg
    31861 tgggtcttga gctggtggtc ttcgcggatc gactgagggt tcactccacg catgatctgt
    31921 tttgccctgg cgaccatcgt ctcgaggtca gtatcatgga agagatttct tgcatcgaag
    31981 gaatcgaggt aggcctggat cttttcgatc atgctgtttt taaagatctt gggctcaccg
    32041 tcttcgctcc tggtgaggcg ttcaacgatg tggttcacgt gggatgcgaa ctcctgacgc
    32101 agggccgaca tggcaagctc cctcgtttcc tccatcatgg cgtggaattt ctccttttcc
    32161 cgctgataga tctcaggagt gaggatgccg tatttgcccg gagtctccag ggtgaagtat
    32221 ctccactcga aaccgaactt gtttcggata tcgaccgggt agtcaaggtc gctaaagaga
    32281 tctccgagcc tttggcgcgc cgtctcacgg gcatcttcgt agcggtcgac aaacttgtct
    32341 gcagcacgga agaaatccgc cttatgtgtc ttcaggcact cttcaaccga gtcgagctgt
    32401 tcttttggaa caagcgtgag gccgatgatc gggaaaggga gggctctttt ctcaaggtcc
    32461 tttcgagccc tgctcacgat gcagcggatg ggatggaggg tttcctggtc gaccaggtac
    32521 ttccttcccc ggagccattc gctgtctccg atctgttcca tgatgctcga atcgaggatt
    32581 cgtgagcctt gccagcagga ggttgaaagc tggatgaggc aggcctgctg aaagatgttc
    32641 tcgttcatgg cttatctcct tgtgtcattt taatgctcca ggctggggca aaatggatgg
    32701 tccccatgga gggatgcttc cctatggcga ggggtgggta ctctcatgaa ctgcctgtgt
    32761 gaagatggtc tttctactca gaagatggtt ttaagcggca ggcggtactc aagtcggtgg
    32821 tggctagccg cagaggggca ggtgcttttt caccacctgc ttctctcctt cgcgctggta
    32881 ataggctggg gtgagctgcc gtgccgtttc cttcccaaga aggtccttga ggaactgcga
    32941 ctcggcgaca catgatttcc catggaagcc tttcgtctcg atcttgacct ctccggtatc
    33001 gagaatgtct ataacgatct ctttcatgcg ttctcctcct tgcgggcgag ctgctccatt
    33061 cttcggataa cgttttcaag ctcggcgatc cagcccagga tgtaggcggc cttttgcctg
    33121 tccataagtt gatcccagaa gctccaaaaa gcagggggaa agtacgcctg cctgatcggg
    33181 atcaggttcg ctgccggctg gacgggcggt tcaaatggtt ccggcatagg gtgtctcctt
    33241 cctaccggga gaatcgaagg aatcgtctct ccaggttttc agtccatgtg tgtttgtaaa
    33301 gcttacctct tgccggcaag cgtaaggcga atgccatgct cggctctttg ctcccggacc
    33361 tggtagccct tgagcctggc ttcacggcgg acccgctcga ctgcataggc ctgcttgagc
    33421 ctgccagcgc ctttgcccag ttttcgctca aggccaccgg ccgagtagaa atcccagaga
    33481 agcacgtatt tgctgccgcg ccgaacaacg ccaacttcat aggaggcgcc tggcacacgg
    33541 atggcatgat cgcagcggcc aaggtcatcg acagtgaatc cttcaggaag cgggtagtcg
    33601 cccacatgct cgttgtacca gcggtagtgc ttctgatctg gaacgaactc aacgccgagg
    33661 cgctcacagg ctgccttgag ggtgtcgaga tcattgatct ccagttctat tttggctaca
    33721 tggctcatct atctctcctc cgcatggacg atgcccttat cgaaggtctt tttcgggaca
    33781 atcgcccagg cacccgggta gtaggtccgc atgaattcct ttgccttgtc catgttcctt
    33841 gcatcgacaa agcgcactct cactctggtt gttgcatcgc gctgatgaat atgaaaatca
    33901 ctcaagctga agacaatgaa tttcaccggc tgacctcctt tgcaaagtag cactgctggc
    33961 aggtggcgga gctcgatcct tccctgaagc atttcctttc gcccaaattg aagagcttcc
    34021 ggcactccgc acacctcgtt gcttcgacct cgacaggata tgcattcatg gcggccatcc
    34081 tatgaggccc ggatttttcg ctgctggagc tcctggcctt cgggagtgtt ggcaatccgg
    34141 gctctgctct ttgcccattc tctgagggct gtaacctctt cccgcatcgt cctcgagata
    34201 ggaacgatcg cgctgaaggc ctcatgcagg ccatcgaaga gtgaatcctt ggcaagctgc
    34261 tcaatctccg agccagtgta gccctggagc ttctcggcat aagcttcggg aataccggag
    34321 ttatactttc ggttcatgat ccgaatgatc tcacggcgct cctccgttgt gggaaggtcc
    34381 acgaaaaaca cggcatcgaa ccttctgatg aactcggtcg gaagcttcga gatgtcattg
    34441 gccgttgcca tgatgagaac aggggttcga gtctcctgga gccaggtgag gaagtgagca
    34501 aacatggagg ccgttgttcc ggcgtcagtc tccccgctcg agcggacacc ggcgaaaacc
    34561 ttttcgcact cgtcgatcca gattgtggac tccccgaagg catcgatcac ccgggttgcc
    34621 tgccgcatgc gccgctcgga ttctccgacg agtgattcct tgagagcacc gatgtcgaga
    34681 cgaatgagcg gccagccgag aatggaggca gtggccttgc tggtgaggga tttccccgtt
    34741 ccgggaacgc ctgcaagaag gatcgccttg agcttcggga gatgctcatt tccgggctcg
    34801 aaggctctag ccctattcct gatgtaggtt ttgaggctct cgaggccacc aacatcgctt
    34861 atgctcgctg gctcccagaa ttccatgaga ccggattttc ggatcatctg agccttgacc
    34921 gccgtaatca ccttcgggtt gaacctgcct tcccggacga gcgagagggc aaaggcagtt
    34981 tccgcctcaa attcggtgag accctttgca gcccgtgcac tgggaagatc ggctgcaatc
    35041 tccaccgggc tcccgtttcc atcctgcagg ctcactccct ttgcaagatc ctgctgcatt
    35101 gagaagatgt ctgcctccga aggcagggga agatccagga catgaaagat cttctccacc
    35161 tcggggcgca tctggacggc cggtgagatc atgatgaggc agcagccggt tgacttccag
    35221 cgcgttagtc cgttttgaat ggcctgaatc acttcgggga tctcgataaa aagatgcagg
    35281 ttgtgcgcga gaagcacggt atcctggtaa tttccgagcc agtttagtgc ctcgacggga
    35341 tccctgatct catcaacgac ctgaggtttt tcggcctccc ggataccctg gaggcagttc
    35401 cagctataaa actgccagcc ttccgtgatg agggtgtttt ccgccctgtg aggctcctgg
    35461 gtgaggagaa gcagggccgg atagccagcc ttgagatagt ctcgaatcat gagcatcctc
    35521 cttcttcaat ctgatatctg cgtttactcc tgacgaattc accccctgac agacaaagat
    35581 cgaattttct ccatcacctc cgcttgcaag ggtgaagcac gtcatccctt cttgtttcca
    35641 ggcaaaggct gcactacaat gaggaagaac taccgaaggt acgctggaga ggccgggtcg
    35701 taccgttact tgcatacagc caggagagca ccatgacttc tttgcttaaa aaggcttttg
    35761 ataaggcatc ccagctgcct caaaacctcc aggaccagat cgctcagggt ctgctcgagg
    35821 agatcgaatg ggagctcaag tgggatgaaa cgctcgccgg ctctgaggat cttcttgaaa
    35881 agctcgccaa caaggctttt caagaattcg aagccggtaa aacggttgag aagggatttg
    35941 acgagctgtg aaatcgcatc tttcaagcga tttcatccgc tgcttcagac aattgcccgg
    36001 acgaattcaa aagcatgcaa gaagaagcta caagctctgg aagaaagatc ccaaccatcc
    36061 cagcctggat ttcaagcaag tcagcagaag gaatccggtc ttctcggttc gggtaggaat
    36121 cggctggaga gcacttggct tgaagcgggg tgaggccgtg atctggtttt ggatcggctc
    36181 atatgccgaa tacgatcaca ttctcaaaca cctgtaaaag cctgcaaccg agcccgtaag
    36241 cgtttgtaga gcaagcgtta cgcggttgcc ctgaactcaa cgagcatgta ctcacgacct
    36301 gttcccgatg agacaagctg gaagggtttg ttgttgatgg ttcctgtgcc gttccttcga
    36361 tattcgccta cgacgcggtt atccctgtag gtgaccttgc cgtctctgaa gtagaacttg
    36421 ttgccgtcga tatagccaat ggcatagcct tttcgtacat acacctttcc gcgtacgatc
    36481 cgctcaaagc tgatcgatgc ttttcggtcg cctttctcga gagtcaccgt ttccttctcg
    36541 gattcttccg catcgattcg gctttcagca tggatgccgg tcttcatgag ctcagtccag
    36601 gcatcggcaa ccggcttcac ttcctcctca ccaatgaggc tcttggcgag aaggacgagc
    36661 atggaagaat ccccttcggc aagagcagta agaccacgat ccgagagatc tccttccacc
    36721 atgagggcca cctgcatctt ggttcccatg agactgagag ctgcttcttg catggttttg
    36781 gtgtaggaga ggtagtagac ttcaactggt tgggtttgcc cgatcctcca ggacctgcgg
    36841 cttgcctggc ggagtgtgaa agtcgagtag ccgcactgaa agaagatgat ggttggaaac
    36901 tcgatgaggt cgaggccggt ttcaaccagg cggggattgg tgatgagaag atcgtagctg
    36961 ccttcggaaa gctttgtctt tacccacgtc tcacggtttt cagccgatgt cgtgttctgg
    37021 cggagtacga gggccgagag gccggagcgg tcgattcgct ccacgatgtc ggggatgagg
    37081 tctcttgtgc cggtatgctc caaatagaag aggactttcc ttccgcgggt cttttcctgc
    37141 ttcaggatct cgaggagctt ttcttcttta gagagcaggt cgatctcgag acttggcgcc
    37201 gcggcgacga taacgggaga gccgtccggc atgagatggt gagggtgttt gactttctcc
    37261 cctctccttg ctccatcagg ataggcaaga agagactgga gcatggctcc aagaagggag
    37321 cgatccccac aggcaagggc ctggcgcact gcacctttca ggacatcttc aaaatcggcg
    37381 taggcttccc gttgttcggc tgccatctcg acggaaatta cgtgctcggt gtaaggcgga
    37441 agatgtgagg agatgtcgga gagcctcatg aataccgagt gctccaggag aaggtcggta
    37501 aagataagag gcgatatgcc ggggcgctcc cttacggtca ctgtccgctt tccgccaatg
    37561 gaatttgagt tatcagagag gggctccttt ataatttctt cgatcactcc atatcgctcg
    37621 gcaaatcgct gcacgctctt atagcttccc gtgacatctc tcatacggtt agcattggtg
    37681 cgccagagga gatagaaaag atttgtggcg tatcctccca tgagggttcc agtgagacaa
    37741 aggaccttct tcgagatgga tgcaaagtct gcaaatgcct gcccctgggc cgagtctccc
    37801 gatttgtact gatgcacctc atcggctatg aaaagatcaa tggccccctt tttgaagtag
    37861 cgtttgacga actcggcctt ggcgaatctt cttaatccgt tttctcccca ggcctgccag
    37921 agcggctcaa agcactcccg gcaggtatct ttccgggtct tcgggtttac aggatcgacc
    37981 agggcgccgc acatcgggca tatgggctga aggtgtcttc tttccattac ccctgatgcc
    38041 cttctgaagt ggttctttga acgctccttt ccgatgacat agaattccct gccggcgggc
    38101 ttgctcagcc gaagatcgag cagctcccta agtcccggtc cattgaggtt caccaccctt
    38161 gcatggggaa cagtatcggt gatctctcgg atccatttcc tgacaaggtg gcccgggcac
    38221 atgatgagag tcctgctctc aggtgggcaa agaaggaagg agaccgcgat tcccatgatg
    38281 gttttcccgg ttcccatctc ggctgtgaga aaaagccctt tcgccccgtt tttcttgtag
    38341 cccttcaaca gggcaagtac cgcttcagcc tgcttcggga aaggagaacg tctcaggctc
    38401 tctatgcgct tcctcatggc ttcttcccag gcaccttccc ccgtgggatc atagattggc
    38461 ctgtttcgct ttgaagcgat ctctttgagt gtgcctccgt atttctccag gaaatccttg
    38521 agagtcatgg catttctcgt gtgttgttgg tgtttggatt tgcgtattgg gaataccagg
    38581 gtggtggcgc gcctggccgg ggaattcatt caatgaaatt cagctgaggg ccaaggcacc
    38641 tgatctcaag tcacgaagaa gcatctcctc gaagcctgcc ggcagctcca tgtactggca
    38701 gataccgaac tgcatgagat cggggaggaa gaatctggta tcaagctctt cgaggttatc
    38761 ccacagccat tctgtccact cggggctaag cggggttgag accagacggt caacagctct
    38821 aaagagccgg gttttggctt cctccagcgt ttttccgaaa acaaggatac gcctttgatc
    38881 cgagctcacc gacttccagg gggtgaatac atcaggctga aaaatgatct tgcttgcaac
    38941 atcggcagta acctgagtga gagcccggag cgggttgctt cctttctgta tccgcacgat
    39001 gtgccgccag ccctctgctt tcctcacatc tgcaccgtgc ccgctgaaga gctgtgctgc
    39061 cagggtcttc agggccatgc tgcttccaaa aagtgacatc cagaggatat ttccctcggc
    39121 atccaggatg tagcccctgg tgtaggcata gactttccca cccgtgtatg tcccgtcgac
    39181 cagtcggtag cgctcctctt ccatgttatc aactccctca ctcgatctcc tctatcctgg
    39241 cttcgttcac acaaagcatc cggactctga tcctgtgcgt gtgaatgatc cgggtgacat
    39301 cgtgcgtctc tgtctcttct gaagatgaga cctcggagac acgtttgact gtgcccttga
    39361 tgaggatctt tcttgtcgca tcctccggat ctgagagctc tgctccgtta cagaagcctg
    39421 ccgcaacgag catggcaaga tgcccctgcc tcattggtgc aaggggaaca atgtcgttca
    39481 tggacggggg ctccacgtgc gcaaagaaat ccttccaaag agtcgagccg gcgatgagat
    39541 cgagaacacg atccggatcg agccgctgtg aacggaattc gagggagctc gctgagtgta
    39601 cctttcccac ctcgcagcgt ttcccggcca tctcacgggt agtgccaaga agcgtcggaa
    39661 gctcatcggg cggcatttct gcaatgtcgg caagcagact gagattctgt tcgaactgct
    39721 ttcccgtgac acttttcttc cacccgagga gaaggagttg gtggaaccgc tcgaagagct
    39781 caccttctgg aaaggtacga acttcaagtg cggaaaacct tgcgatctct gacctcagaa
    39841 gctctgactc gagagcggag agaggaatga ttatgacgat catcccgagt ggagagagca
    39901 cccggcggta ttttgtgagg aagcgatact cgatgcgctg gttttgctct tcaccttcct
    39961 cgaaatcata cggtgggttg agaaagatga agtcaaagcc tgagctcacg ttgatctctc
    40021 tcagtgcatc accccagagg agatggccaa gcacttgctg ggctttgctg aggcgctccc
    40081 gttcaagctc gataccgtag gtttccaccg catgcccctg agctatgagc gccagtgcgt
    40141 caccttcgcc gcagcatgga tcgagaaggc ggcagccagg ctcgaaggcc acggactgct
    40201 tcacgtgttg agctacttcc aggggagtag cgtagtagcc gagtttggat tgcgatgcga
    40261 gtcttgccat ggaattaccc caccccattt ggaatgatga atgcggaatg atgaatgatg
    40321 agtagatcat gctgtatcat tccgcattca tcattcatca ttgcttaaaa gggaatttcg
    40381 tcctcgggca aaccaggagg aggcagatcg tcagggggtg cttcataggc atcgcttcgc
    40441 ctggcgctct ggttctggga accggctttc tgaccagcgc ccgtgctccc gttctggccg
    40501 tttccattgc cgtttccgaa aagctcgagc tgccttaccc tgatctcgac agagcgccgg
    40561 ttgttccctt cctggtcctg ccattcacgg gaaacgagct gaccgctcac gcctaccttg
    40621 gagcccttgt ggaggtactc gccggcgatc tccgcagtgc ggccgaaagc gcttgcacga
    40681 aaccagtggg tacgcttttg ctgctcaccg ctctcatccc ggaagtgctc gtttacggca
    40741 atgttcaagt tcgctacggc caatcccgac tgggtgtacc ggatttccgg atcggctccg
    40801 aggtgccctg caatggctac gatgttcatc atggcttcac tcccctttct tgaatgatga
    40861 atgatgagtg atgaatgcgg aatgatggct cacaattcat cgtttatcat tccgcattca
    40921 tcatttgatt gcgtctctct ggtcgccctg atcccccgtt ttgcgggtga tcttcgagca
    40981 ggcaggggac acttccctcc ggacatctag cctgcactct gccggaggtg agagttcgca
    41041 atggatagac ctgaattgga ttttgttctg tggcgagaaa ccggatcata cgaaggcatg
    41101 tggccgtggg aaagcttgcg agatgatgag ctttcctctt ccattctctc ccagaccttc
    41161 attgaccttt tcgcaaagaa ccgcccgaag cgctcgcatg cactccatcg ggttttcacc
    41221 ttcctggatt tctgctgtgg ccgagtggct cacactccag gagttgaagt ttcgggtgac
    41281 cttcttgcag accattacag agatttcatt tatcttcacg tgccggcctc ctttctcatg
    41341 aagggttcat ttcacttcag gcaggcagca atcagatttc ttttggaggt ggcgaactga
    41401 aagcgggaga tgttgtacag gccgggaagc ccgactgtct attgagcttc ccggatggtg
    41461 atcttctctc cttctacggc aacggttccc ttcaaccgct tcttcccgat cttcgaatag
    41521 tcgtttgagg agatgaaggt gatccgccca ttcttgtgga tgaacccttc atctcccctg
    41581 gcggtgctct gaggagtgaa cgtgaagctg atggtctcgc catcgaagag ctccggggcg
    41641 ttggcaaata tctttgcttc caggccccgg atgtagacat cggttgactc gccgtaggat
    41701 ctgtagccaa ggaaagccag gggctggatc catccaaagg agtagttgat gatcttgagg
    41761 gtccggttgg cttcctttat ccgctcacag ccaaaccgga ccgtctccca atccctggct
    41821 tcctgggcgg gccaggttcc gaaggtggcc atttggatgg attcttcgag catgaaaagc
    41881 gaaaaggtaa ccagccctgt aaccgagatg tagagagcga ggatggagag aatcgccttg
    41941 gcccttctgg tgatccagga tgggagaatc atcggatgat cttgcggaga gtgtaggtgg
    42001 tgtccccgcc gttatctctc ttggagacaa tggccttcat cgtttcaccc tccttcacct
    42061 gtgaggcttc gttcttggat gctccgaagg caaagaccgg ggcttcggcg gtgtaggcaa
    42121 tgccgctcaa ggtcctctcc tcctggacga agatgatgac gtaggggttg ccgtccttat
    42181 tgatctttga ggaaaccctt tccaccttcg tgttcaggat catgtcctcg gcatgggcaa
    42241 ggaccggcat caggaaagcg aaaaccagga taagagctgc aatcttcttc atgttgggac
    42301 tcctttcgtt tcgtgctgtt gggtttggga tgtacttcga tcaccggctc cggatgattt
    42361 ggttttccgg atagactccc tgtctcctcg ttgcatcacc tccttccatt caccaagggc
    42421 agacaagaat cagatctatt gcccgttcga ttttccgccc tcctcaatgg cttttcttgc
    42481 aagctcgtct gcccgctcgt tttcgggatg gccattgtgg gatcgtaccc attgccagtt
    42541 gacctgatgc ttcgcggctt ctttctggag gctccgccag agctccttgt ttttgacagg
    42601 tgagcgatcg ctcgtgaccc agccgttttc catccatctt cggatatact gggtaatacc
    42661 gtccacgaga tatcgggaat ccgtgaggat cgttacggta cagggttcct tgagcaggcg
    42721 aagggcttca atggctgccg taagctccat ggcattgttt gttgcctttg actggaagcc
    42781 tgagatttcc ttcttgtggt ccttgtagca gagaacagcc ccgtatcctc cagggccggg
    42841 gttccccgaa caggcaccat cggtatagat ctttacctct ttcatggctt acctggtctt
    42901 tcgggattag atcgatccag attggttctg attgccaggg ttggttgaga tcgctgatga
    42961 tggactgaga ttggatttgt tctggagtga ggtggtggct ggcggagtcg gggcccccac
    43021 cgggatggag ttcattgtcg gcttcgtcta tgcgtaaaga atcctgtagg cggccgtccg
    43081 ctcccacact gtggcgatgg atcttgccag ccgagggccc aggattccag aaagcctgca
    43141 gtagacgtgg agcacattga atctgtgctg gagaaatgcc ctcatggctt ggtcccctcc
    43201 tttcgtgcac gtgaagggtt gagggcatat ctcccccaac ccgtaacgtt tcattctcaa
    43261 acacccgcgc agcactcgac cgtgatgccg tgcctacagt gctgcccggc cggaacagac
    43321 gtctcccatc gccatgctcc ctgccagcac tgaacgggaa cccagcggcc gcacccgaga
    43381 gaatggatgg cacgaggtcc atcggcagag aagagaacga cttgctttcc gatcctggcc
    43441 gcctgctcac aggtaaacca tgtgcccttg ctctcgccgg caatgaaagc aacaacgcct
    43501 gatgaagcgc gaaccagatc caccgtgcga gaaaagagcg cccggatgta tgtttcacgc
    43561 tcgcttgcat ttcctgccgc ttcacccgaa atgatcctgc cgccaaatgc ttcgaactgg
    43621 agaagagcag tgcggcattg tgaaggggcc tgaaggagag agccgggaag atagacccgt
    43681 gagctctcac aggcttcgcg cccgagttga actgcggcct gcaaagcgag aagatcagcc
    43741 cccagggctc ctcccgagcc aatgcgacac ccacgccgga caagctgact aacgacctgt
    43801 gacaccctgc ctgcccacga tgccggaagc tcccttgagc caacaacagc aatgagtttc
    43861 atggcaaacc tccctggtaa aaagacacag caaaaagaca aaggaaattt gctttggaca
    43921 ggcagaaatc agattttgat gggggagaag gtttccggct ggcaaatttg tgcctgaggc
    43981 acgtgtgtgt tactacgcaa gccccaggaa gagcatgagg gatcggttga cgcgaagcag
    44041 tacctcatca tccaatgaac cgattcgcgt acggatttta tctttgcgga tcgccatgat
    44101 cttatcgacc atgatctgag aggaagcctg caggccgttt ttatccgagg gttcaacggt
    44161 aatgcgaaag agtggagcat cctgaatgtc gctcgtcatg aggcagacaa gaatgcttgc
    44221 gtgggtatcg ttaaaaagat ccgattgcac gacgagtgcg ggacgaggct ttccgtagtc
    44281 accggctgca actgcaagga caatgtcgcc gcgcttcatt tccacccgtc cgtgtcggcg
    44341 gcagcctcga tgaaatcgag tgcttcctct tccgaagggc ttcggctcac cagaagagac
    44401 tgccttcggg cttcctcggc gaatcccgga gcactggtat cgggtaccca cacctgcacg
    44461 gggcgaagtc ctgctgagcg gagccgctcc cggtagcgct gcatcttttc tcgggctgca
    44521 tttgcttttg ccatattgtg acctccgtaa tcatgtcttt aaccaatagt aacatgttac
    44581 ccgaatcaaa gcagcagttt tttctagatc gcaatctcct cttcaggaaa atcgaatgag
    44641 cctggctcat cagaggtccc gttagaatcg ctgtccgaaa taccaggtat ggccgcggcc
    44701 gtgtttccat tcgagtcatg gaggatttca gcggggacaa cgccggcaag ctcagtgccg
    44761 ctatcgagaa gaattccgat catttgatcc tttttgagac taccgatact cgatttgagc
    44821 ttgagcccgc aggattcgct tgcgtacttc caggcatcgg gcctctcaag gatgttgaag
    44881 cggttaatga tgctaacgat ttccgctttc gtcttctttt cgaggtattc cctggtaatc
    44941 ctccactcgg cggcaagatc gattccaagg aactctgcaa cgctgcggcg ggtagcgggc
    45001 atgacagttc ggttgctcaa gagaacctcg attgccgctt ccttcaaaag atccactgcc
    45061 gggccgggat cgagggcttc gattgcggga aagatgtccc tgcagagcag gaagaagctg
    45121 taatcggtat ccttgggtct cttcgggaga ccgagccttt cggcaaacca gcgatggatg
    45181 tagctgttgc ttgcgatgat cgcaaagagg cttagctgga tcatcttctc gtgaaaggga
    45241 tcgtactcct ttgctttctc gggaatgcga tccttgaaga agagctccct gaagtattcg
    45301 ccatgccagg cctttgccgg tgtaccgtct tgattctccg tcccgtccct gcccgtgaga
    45361 gtcttttcac ccgtggaagg agactgagtg gagccggtcg ttgggctccg gctgtagagc
    45421 acttcctttc ggcactcaac cctcccacag catgcgcgat tgcgatgaat attcccgctg
    45481 gggctcagga ccgtcacata gttttcgcag gataggcact tttcggctgc gttcctatag
    45541 tagaagggct cacagtcagt gtgggaaacc tcgtcgctga atcgaatccc gttcgtattg
    45601 gtctcgggat caagtgcctt aacgtggctc tgccagtttt cgatcagcca ttccttctgt
    45661 tttgcatgat agcaccggct gtcgaggcag cgggcatgat cgtcttcaat cccgaagagg
    45721 ttcttctggg tctcggagtt tttggcgcac atgccgcatc cctgtttgtc gaagagcgca
    45781 cgggaaagct cagccgaggc atcatcgatg cgtcctttaa gttccctcac ggttgggatc
    45841 ccctcatctt cagcccaggc agcaatgatc tgctttagca gcatgagccg gtctttcttg
    45901 ccctctatgc ggaggaactg ctcgaggtgc ccgtactgga ggatctcctt ttgccaggcc
    45961 cagatgagag gctggggaag ccgcatgatc tcaactcgcc ttctcacata gcggggcgag
    46021 accccgagtc tttctgcaag ggtgactacg ccgtccgctc ccttctggtc gacgtattcc
    46081 acaaaggcgc gggcttcctc cgcctcgctc aggtcctggc gctgcagatt ctcgatcaaa
    46141 cagatctcga aggcctcatc atctccgcac tcccggatga tagccggtat ggtggagcgt
    46201 gcggaatctt gcgatgcaat gaggcacatc gcccggtggc gccgctcacc ggcgaggatc
    46261 tcaaagtcat gcgcctcctc ctggatggag cggacaagga tcggctggag aacgccctta
    46321 tcccttatcg acgcaacgag ctcatcgaat gcctggccat cgaatcctga gctgcgtggg
    46381 ttccatttgt ttccccggat tcttcccacc ggcagttcct ggaagtttgg atgggtctgg
    46441 ctgggaaggt cttctttctt ccttctggtg cgtttcttcc tctcctctgc catagcttct
    46501 ctcctttgct ccggcggtgt cccggccctg gtcctggttc ccgccttgat cttgtttctt
    46561 tccatggcat actgcatgga cagctctcga tgagtcgagg tgtccatcat ccgggcgtag
    46621 agcgccttgt atgccttcca cgcacaactg aagcaaatcc cccagtggct ttcaccctga
    46681 agatttgcca gaggaaccga tttcttcgac catcgacttc tttcttcact ctggaaaact
    46741 gtgcagaacc agcacccacc ctttgcagtg atgctgttga gaccctcgag gattgcgcgg
    46801 gcgcattcat ggcagatccc gattgcggca acccggatgc tgataatggc aaggacgtgg
    46861 cggtggcaga aaatacatag aggccgtgca ggaggtttgt ggctcatggt agccttcctt
    46921 agtgcgggtg aatggaagag aaatatcacc cgaggcaggc tgaaaacaga tttgagggct
    46981 taatgactac tcgatacctt cagcgctcat ccaatcaata caagatttca ccacatcccc
    47041 gtgacaggct tccggcttac accaacagat gagataaacg tccccacatt cagacatttt
    47101 cttcattctc accagctctg caaacactct gccccgagct ttcacctgct cccaaagcca
    47161 tactctatat ttccggatca cttcggctct gtctccgtct ctaccgatag caaacggatt
    47221 ccccaagaca cttggccttc caatatagat gccttcaccc tcaaaccgct tcttattcac
    47281 cacatagact cccatagccg acctcctttc attcaaaaga ggcagcctgc gatcatattt
    47341 tgagagccgg ggacatgggg cgcgggcagt gagtgtcata ccactgctca cgctccatcg
    47401 cgggcatata ggggcgggtg gtcggggcgg tctgggcata agaaagaaat ttgaagatat
    47461 gcctcggaag ttctggggca cgataatggc cgtactcgtc tgctcctact ccgtctcgag
    47521 atcgaccagg cgggatatga tcgtcatcct ctgcagaaac tctctcgtcg agattttgcc
    47581 gaaaagaagt cggcatgctt cgacaaagac ggcgctatca agctcggcat ccgagagatc
    47641 ccgcccccga agaatcttct cacgcaggat gctcgtcgtc tcaccacctt tttcgcggtc
    47701 ttcctggtca atctcatcga gcaccttgtg aagaagaatg aatatatcct tttggtgctc
    47761 caggaacttg tggtaggcgt catcccgatc gagcatcgct tctatctcgt catgggtgtg
    47821 ctctttcgaa atcccagtat ccgctcgggc aatcgggtca aaatcgtcat cattcatcgt
    47881 cgtatcctca ataaaagtgg tcagtggtcg tcgcctgaaa tttccatcat cctattcatt
    47941 aacaaagaga tcgagcatag catctgaggg tgcatcatca ggctgcgaaa aaataggcac
    48001 aggccgcagc cgatgaccga ggaccggggc gtttcgcccc agttcttccc actggcggtg
    48061 atcgggggcg ggcggtgggg gcggtctggg cctatgaaag attgctcgtt catgctgcgt
    48121 cttatgcatc tcctgttcag gatcattgaa tcccgagctc tcaaggcgtc ggtgatccgc
    48181 ttcttcatct cttcctggtc caaggagccg aaaagatcgt tcatggtgat ccagaagaag
    48241 ccctacagtc cgtggacgtc accggagtcc ttgaaaatct ccaccaaaac ctgaaattcg
    48301 cccggcaagg ggagcacctg cggccgtaac ccgagtagca ggagggcggt acaggggaag
    48361 acggctgggg ggtccccagt ttttcttccc ctgtttttcc ctgtccttcc tgcttctcct
    48421 gttatccggc tgcactctgc ttccgggtgg gcggaggggg ccggaaggtg ggttccctgc
    48481 tcggttgttt ggtccatggt cgctttcctg atgaagggag cgggcggcta acccgctccc
    48541 ggtctgtgct attctttggg ggtgagctta tctttggctg cattgtaggc tgcccaaatg
    48601 aacttccatt caacccgctt catttccggg cctttgattt cacccttttg gaacttgtag
    48661 aggtttgctc caattgaggc aagctgctta gagtttcttg cctcacgaat acggcggatg
    48721 aaggcctttg caggctttga catgaatttt tccgcgcggg attcgaggta atttttgcgt
    48781 gcattgtaga aggcaaggaa aacactcatg taatcaccgt gaaggtgcgg gtagacttct
    48841 ttgcagagct gctttaactg ctcaatctca gtgcaggatt gaactgtatc gattagcttt
    48901 tgaaaccagt acgggcgcga ttcaatccag ccggcttcct cttcagaatc gagggctacc
    48961 ttgtgatagg ccatagtttc gggggcgctg tactctgtgt gtgaatggca ctctaaaaca
    49021 tcttcggggc ttatggcttc tgcttctgca agggcaagtg agaggcgata gaggtactgt
    49081 aacgttggct gcgctccctt catgcgggac cagcggcaaa acatggcggc tatttcttcg
    49141 ctagcttcaa ggatagagag caaatccgca aagtgaaccg cttcgcgggg cgtgcaatga
    49201 gcctggtgca tgagctcttc cgggcggaga tggcgcgggc aggtatcggc gcgctcatgg
    49261 ggaagcatgg gctgtgattg ctcaaagggg gtgtgctcgt aaatgtcgcg ctctgattgt
    49321 gagacaaaga cgggcgtgta atccggtgaa atactgaatg cctcatctaa ggacacaagc
    49381 cggatgccgt ttccttcatc ttttcccggg tgcggttgct gccaggattc tccttcaacg
    49441 tggcaaaagc tgggccatat gtcgcccgct tcatgccatg cgtgaatttc aaggctccag
    49501 tacgggccat agtgcccgct cttgagctcc cggtgaatgc cgttttcata gctgatggct
    49561 tgcatggtgc gctccttccc gttccacgtg gaacattctg aatgattacg gtgcgttaat
    49621 caaaaagaac gatttaccat tgtggcggct gtggcgcatg ggccggtgtg cgtgtcaagg
    49681 ggtcggggct ggcgcgatgg ttgcgctgca tggggtatgc cagtggggct ggcacggtgt
    49741 gtgcgctggt gtgatggggc ggtggcttgc gggggtaaca ggcagggcag acagggcggg
    49801 cgtctttttg cccggcctgg ctgccctgcc cccccgcgag acactgcccc agcgcaccag
    49861 catcggcaca caccgtgcca gccccactgg cataccccat gccccacgca accagcgtgc
    49921 cagccccgat ccaccgcgcc ccttgacacg cacaccggcc catgcgacac tgccgccacc
    49981 aaaaggttct ttttgttcat ggaatcaaag ctcaccatgg atagacatag attagattcg
    50041 ggtaactgat agttcaggcc tcatggcatt gcgggatgaa agctggagcc ggaaaacatc
    50101 gctttttacc tgcaactgct gtgacgttta ccttctcatc caattccccg gtaccagttc
    50161 tgcaaatatt cgccggcggt ctcctctctg aaccgaacac cctgagcctt cctcgtatcg
    50221 aaccattccc gaacggcatg ctcaaaggcc cgatacattc cgggccagcg tttgcggttc
    50281 ctctcaatga gcttcatatt ggaagagcag atgaagggac atacgacaca tccaacccga
    50341 tcccatccct cgtcatagag actgggatac gggagatcgt gctcctcgat gaactcccag
    50401 atggtccatt ccttccagag aaagatgggc ttgtaaacga cagttccctc tctcctgttt
    50461 cgctcgatcc atggcctctc cgccctgctg ttggattcct ctgcccggat tcccataatc
    50521 ctgtgcttta acggaatgtt ccttgccggg tatttcctca gatagtcaca gcaccatctc
    50581 ttcgtccgga gcggtgggaa cttcttcctg atcccatcga agaagctcat ctgcggatag
    50641 aggaagcgaa catccgggta atgctcacga atgaaacgga cgacctcagg aggctcgatc
    50701 cccgagcatg aatagaagga ctgatgctta acgccagcca tgcgggtgag ctccaggctc
    50761 acgatgctgt ccttcccgcc gctgaaggcc acaaaatagc cctccggcgg ctcatgagct
    50821 ttgatgaacc gaacggattc cgaaacaatg agatccagag tggatgccct gcgccgatgt
    50881 gttctcatct ttgtacaatc cttccttgag tctgcgagcg aaactcatcg atcaaagaga
    50941 ccagccatgg aatgtccggc atacaggcct cgagctcaaa gacgtgggtg cggatgaagc
    51001 gggcaagatc cgccggccgg attctccagt aaccgcttcc atccggattg tccttctcgg
    51061 ccttgagcat ccccgaactg atccaccgct tcaccttctc cgaagagcac cgcatggcta
    51121 cggcgacttc ctcattggaa tacgtgtccg gcttctggcg aagatgggct gagcggatct
    51181 tgcccttgat ggcaacaaag ctcctcgtgt agcctctcga cttcaggatg cgctggatcc
    51241 tgatgatcgg tacctcaccg gcatactttg cgatgactgc ctcctcttca tcagtccacc
    51301 gcttgagctc cctgctcacg ccgatcacta tcgccctgtt ctggatctga gctttggggt
    51361 agccgataaa gagaggatgc ctggtgagct tctcgagagc accccggaag tagtgcttct
    51421 tgtaggcttc gcgaatggcc tgatcctgct cctcagacca ggtgtatttc ggtgttccca
    51481 tgtcttcctc tatgtagtgg aaatatcaaa agaagctaaa aggatggttt aaaagggtat
    51541 caagaacaca tctgaaacct caaaacccaa tgaatcactc aatcaccaaa tacccatctc
    51601 tccccctata tccccctctc ttcccaaagg ggtggttaga ggaaggttga agatcgcctc
    51661 gctgacacga aaacgatgct ccaggcaggg tgattttcag gggagcacgg ggggcgaatg
    51721 ggaagcagag ggggtggtgt cgcatggcgt cgagggtcca gtgtggggat gattcgattc
    51781 atcaggggtc tcgctccttg atctttcttg catcaggatc tgctctggcg gtcgctgcgc
    51841 gggcgcagat gcaagttcct gccgaagagt ttcgagcgct ttcatcgcct ggtgtggcaa
    51901 ccgcttctcc cagaatgggg cgtaacgatt ccacatcgcc tcgtcgcttg cagttctgat
    51961 cttttcagca gcgaagagga agagtgcctc atcgaggtag gtgacatcca tgtcggtctt
    52021 tcctgatccg agaagggagt gccagttcct ggcaccttcc tgctcttgtt cagtaaggaa
    52081 cgagaagatc tctggcttat gcctggcaat gaggtcacgg atctcggaag tcagaagatg
    52141 cttcggggag catctcaggg cggcttcatg cacacgaatt gaaatcccgc ggctatcgag
    52201 tagctgcagg atattgctgg ggctcaatgg gtccatcatc tttcgaggtt tctcgcttaa
    52261 tcaacttttc gtctttagac caaaggggga aaagatcgtc acaatcgtca cgaccgtcac
    52321 agctgccaaa aaccggtgta atttcaatcg gctttgctgt gacgatccct gtgacgatca
    52381 tcccgcggga tcgtcacaga tcgtcacaaa gatcgtcaca gatcagagtg tggcatgatg
    52441 cggttttgtg gctgcctgtg acgatcgtga cgattgtgac gatcttttcc gagtttgatc
    52501 taaaagggga aacgttaaga tcaggatcat gatgtctgac ggctgggcat tcacattctg
    52561 ttacagctcc tcccagtcat ccggcgccgg cgtgccggtt gattccgttt ctcgccgaac
    52621 attgtcactg gtggtcttct cccggtttgc tttaagcacg atctttcggc ttccagatcc
    52681 ggagagtctg aagaattcca cctcgatccc gactgcccga aggaatgttg cagagcgctt
    52741 cagccggttt gaaagtgtgt ttcccctctt tggccatgac tgcgatttct ggatcttctc
    52801 ggatacgatc tcagagagct tatcgagaag ctccgacgag gttccctgcc attcgtttcg
    52861 agaggccatc aattctctta tggccgagga gacgggatcg gactcgagtg agagctcaac
    52921 tgtttctttg cggtttccgt agtaggtgtt gaggaattca ccctggctcc agggaagagc
    52981 aggctccgcc gcactgaccc agagtgcgaa gtcagccatc cggggatgag ctggaagcct
    53041 ggtgtgggga aggtttttaa gggctacgct tacggcatcg cagagtgctc caaggagctt
    53101 cgggtgggcg gcctgaaagc tctcgattat ctccttttca gggattcttc tttcctccgg
    53161 gatgggcgga aggtgcaaaa tgatggacct gtctgcaaga tcgtgcctcg atgcaatctg
    53221 gtcgattccg gtaagaagga gtggccgtgt ggcattgaaa atcatctcct catcgtttga
    53281 gtagagctcc cggacggcat agcctcctcc cgtagagagc ctgcacaggg catctgaaag
    53341 ccagagggga actcccgaga gattatcgaa ggcaataatc cagccattgg ttgctgagat
    53401 gagaaggtct cgttcctccc ggggaatcgt tcgtatggga gcggtggatg gatctatgag
    53461 ggaccgaagg agccttgccg ttatgctttt ccctgatccc tgctctcctt cgaggttcaa
    53521 gatgggatag ggtccggttg gatgcgccgc ctggatgatc cagccaacca tcagttttag
    53581 agctgcgtcg tcgggcagat tcaaaaatcc cttgaggcta tggagcgatc cgccgcgctc
    53641 cgggtaggcc agggggagca tcccctttgt tctgcggaac ttgactggcg gattactcac
    53701 gatactccag cccgatggac tgatctcgac tgcctcccag actgtatttg caagatcgat
    53761 gtagatgcaa tcaccggatg cagcaatgcg catgaagact tcctggagct ctccttcgaa
    53821 ctgccctttt gcctccagga tgttgaaggc atcctggaga gcctggtttc cgggaggctt
    53881 ctcaaagtcg aggtagaacc tgtgggcaag ccagcgccga aagctcttgg agcgcacgga
    53941 ccatgtttct ttgtgctcat ccatcggaaa ggtggcatag atcacatcat ctgccgtatg
    54001 gaaaagctcg acatcaccgg caagaagtac aagctgctgc gcctgggtct gctttttccc
    54061 attgccctca tccggggatt ctgcctgaac tgccttcctg gatttccgac tcgcatacgg
    54121 ggcggtgata cgttcaatct catctttggc tggaaccttc gaggttttta tcatctcctc
    54181 gatgatgcgg gagtgaccgg cgcggaggag atcgtttaca tccttcattc catcgggagc
    54241 aagggaccag tcaacaagcc tgaagacagg cccgaacttc atgaggatct tggctgtggc
    54301 ttcccttcct gcctcgtcat tatcgagaaa aagaatagaa gtcgggcgaa gatcgatttc
    54361 agcgagttta ttgatgagcc cggcgccaag gagacagatg gcttccgcat cagggagggc
    54421 ttcggcaacg ctcaaggcat cgacaatgcc ctcgcaaaag accatgaaat ccgttcccga
    54481 tccgaagcga aaaaaggccc gcttcgagtc ggtgcctttg atgaaccgct tcttgtttcc
    54541 atccggcggg atgatctgta ttccaaggat tctctcctgc ttccagtcga aaaggggaaa
    54601 cgcaatgccc ctgagttttg aatcccatcc aaagatctcc cgttccttga gacgctgaag
    54661 ggtagccgag cttatccctc ttctttcgag gtagaggaga gcttcttccg ggagccctct
    54721 tttcactgcc tcctgcatgc gccgggcaaa gtattccgag ccgggaccgg tcgctctccc
    54781 tcttgagtgg tccatctcct gcggatcatc aagacctgac tcagatgcga gcaacctaat
    54841 cgcttccccc ttcgagcacc ccacgccccg ctcatagaga tcaatggcat cgagggagat
    54901 tccctctgca tggcagtggg caagatccgc tgtgacctgc atggagggtt cacggtcgtc
    54961 atggaaggga cacaggctat ttcccctttc cctgtatttg tccggccaga agcgtttgaa
    55021 aatgctcctg cagtcggctt gagctttgat ggatgaaatc agctcctggt atcgatcgct
    55081 catggcttaa atacccttaa cggaatagct ggccttgtct gtcgcaagtc tttgatgggg
    55141 aggtactgat tcaagtggcc ggtaagcggc ctggctttta ggtggctcta tttttctttc
    55201 taagtgaaag ctctgccggc caagctatgg caatcaatac ggcgaaagac cctgcttgcc
    55261 gccggtccaa gccgccggtg ttgagtcctt ccgacgatta agacgagcta tgcgctctgc
    55321 cttttcctca ggtgagagga tgcgcttttg tggggcggta gctttaagcc aatcggctga
    55381 aaggggttta tcaatatact tctgaggctt caggcgattg agctgctttt gccttcgaat
    55441 gatgggatcg tttgtttgat ccctggactt gcagacatct tcgccgatga accaggagtg
    55501 cagagcgatc tcctggtcca aaggacagag attagcgttg cacgaaatga agtgtttgca
    55561 gctcgaaggc gcctgttctt ccattagact cgtctcactg tcccgccgat atcggaccac
    55621 cattttgggt gaaccgtatc atctctcaaa aagcggctga tgatggactg cacccttccg
    55681 tcattcagga aaaaggcgtg agtcgcccta tccgtccact tgctctgaat ggaaagggtg
    55741 aggatgtgat catgaaaggc ggttgagctt tcgaggggga ccgttccatc tccgtcccct
    55801 gcaggctggt tgaaatcaaa gaagtttctg acttttccgg aagggttttc aggagagaca
    55861 acaacccagg ctttggtttc ctctccttcg gccacaagga tgaggaatct ttctttcacc
    55921 tggggtggaa gctcgcttag agggaacatg gccgggttct gcggatctct gaaacggcga
    55981 aatgcttcga gtctttttct gaagatggga agatccgcaa tgttggactg ccagtggtcg
    56041 ggattgaaga tatcgaagga attctgttcc ggggcaccga agagggtaac ggcattggga
    56101 tagaccgggc atagctcata gacggatggg aaggagcggg cgatctttct gaattcatca
    56161 ttcccgttga agaatgggaa cctgcttcca ccttcgccca caagaaggcc gatgagggct
    56221 ccgaccgagc caaggaaggg gcatacggtt atcactgcat ggttgatggc atcgaaattt
    56281 tctttgaact cctttatgaa acacgagaaa accatcccac ccatgctgtg ggtgaggaaa
    56341 ttgaagcggc tgatgcagag cttctctcca aggtactcga cgaaggcctt gagccgtttt
    56401 ccattctcgg cacaggattt tcgccagtca tagccaaaaa tgaaaaccgg actctttatc
    56461 ttccgctcca ggatgaggac ggattctttg taggccagat cctcgacatc tcctctttct
    56521 atgatcacac ccggtgcggt ctcgaaccgg gtatcttcgg taagctcgag atcgaggacc
    56581 gtctcaaatc ggcactggag accggaccaa atggtgtcga agctcaggct gttgctgtta
    56641 atcagggttg tccctttgat gccgggaatg aggattgttg gagctaccat gattcaggca
    56701 tcctcccctt tttgttctgg attgccgttc tttgcggcaa gggctcttaa gccaagcttc
    56761 ttctcgtaga ggagcgtaaa tgccgagagc gcagcaggcc ctgcaaatcc tgacatcccg
    56821 atcacgaagc tttggagagc cggagaaaga tccatgcacc gcaccaggta acttgccatc
    56881 acccccgtga agccggaaac aaagcagcat gaaataaact gatagatcgt gaggcgcttt
    56941 gaggccatga agagcttggc aaagcctccg aagatggcaa gcagtgctga tatgatgagg
    57001 atcttcagct cattcatcga gtagaagttg tcgccattca tttacatatc cccgtgggca
    57061 cgcggactgt cttcgcaatt tcgcaatcga taatgcggtg gtgcctatgc ggcaagaatg
    57121 cgtgccttct tgaacttact gacagtttca agctgcggga cctgaatgaa cccgcccttt
    57181 cggatcccct ttcggatgag cttcagcact tcgttcctgt tgatgtttcc tttcgcctgc
    57241 cttatgcagt tgcagagata tgaggtgaaa gcaccgccgt atcggccgtc gagaagagca
    57301 tcggcagcat actgatcgga gcggcaggcg gcccaaagga ccttgatctt tgccggcctg
    57361 atagccttga ggcacattac gccgggctct tgagactggg gcagtagctc atcgggaggg
    57421 ctgagaaaac gcctcctcat gtagttggca ctcttgcctc cagcgcccct caacccggta
    57481 ccggagtggc agcagtcgag aaagacctct gcgtgcaccc caggcttgat ggacttaaag
    57541 atcctgctca aatcatcatc ccggattact ttagactgcc agtcaaggtc ataggggcag
    57601 atgatctcat cgcacctgtc gggctcatca ccgtctttgt cgctcacctg agagccgtgg
    57661 cccgagaagt gaaagacgat ctgatctgcc ggttttgcac tggataccag aagatcgaga
    57721 gcctcgagga tattctcggt agtggcgtcc cggtcggtca ttatggtgat gttctccgga
    57781 ggaaacccga aaagagagac gagaagatcc tgcatgttgt ggacgtcgtt cacacatccc
    57841 atgagatcgt tctgtttacc ggggtagtcg ttgatgccga caagaagggc ttttttcatg
    57901 ggttacttcc tttcttgcgt ttgaaatacg gttattcggc aagtccttcc gaaagactct
    57961 gactcataga tgttcttggc ttcagcctga aatccctgcg cttgttgtag acattctcct
    58021 cgatgaccac gactgcatcc tttcgtatgt agcctggatg tgtgatgtga acattcatgg
    58081 tgcaggccgt gtccacgatg gagtactcgc ctttggagcc tgtaacgacc ttcgataaag
    58141 tgccgtccgt tcgaatgact gctccagcaa tgggtgcacc gctagtggaa tctgagacta
    58201 tccctgtgat cattcccgtt cctgccatgg tcacatcctc cactttgggc cttcgaaggt
    58261 ggggagacag actttgtgtt gtctccccaa acctttcaaa ccataaagtt ccttgccgct
    58321 tcaccgtcaa tcttctcatt gccgatgccg tccccatcgt catcgagcac tgggatctgt
    58381 gtgaggtatg catatcggac cgcattcttc gcagcgagga aggcctctct caggctgtca
    58441 tcgtcaagga cctcggccca gaactgctgg gagaaggaaa ggtcccctga gcaggcgaag
    58501 tttgctgtgt tgaaagcatc cgtgcttgtg acgacaatgc gggtctttcc ggccggaggg
    58561 ggagaaagag cggggaggaa gcttccggca aagggtgaat cgatcacgac gcccaccttt
    58621 ccttcgtagc ttgcctggag cgaatcgaga tagctcttga gtgttgcagg agtgaggttt
    58681 tcagaggcat tgagcttgaa ggagccggca gcgccatggc cgaccaggta gatgagagcg
    58741 tttgcagctc ccggaatcca ggactggatg gcactctgaa ggtttgcgag tgtggccggt
    58801 gaatcgtatc ccggaaggcc cgaaggtccc agcatcttta tgtttgcatc cgagtatccg
    58861 cgagacttga gcacatggta ggcgaggcct gccacatgct ttatgatcgc agtcgtcgga
    58921 tcgttgccgc tcgggctgga tgcctgggct ccggcgacaa agatcacctt ctgggcggat
    58981 gcggggagct gttggatcga tccggcatcg acataggttg aagcgcagat ggtgccgtca
    59041 cctgcttcat ttccgatcat gtttccgttg tcgtttagaa cggcagtctg cgtgcaggtg
    59101 aagccgatag ccgccttcgc cttcttgaag gcagtccaga gggaatcacc tttcaggatg
    59161 cgaggccaga agaaacctga aaaggatatg gaaccgtgat tgatgaagta gccaaactgc
    59221 gagtcaccgg taccgcagat gatgatgcgg ttggctgccc cggagggtgc taccaggttg
    59281 tctacgaagc ttcccgagaa gcaggaatcg attaccacga ttatctttcc ggatatcttc
    59341 tgctgcaacg cgtcgagcca ggagtcgagg gatgctgcat ccagagactg gcagggactc
    59401 acagtgccgt ttatgagaag actgtcgttt cctccatggg tacaaagata gatcaccaca
    59461 tccgttgcgc ctgcagccca ggtggttatg gcctgctgga ttctggcgca ggatggatcg
    59521 gcatacctct catcgctgat cacgttcttg tcgaaatcgg gaggggtacc aaaggtgaga
    59581 agacgaatgt tgctcttggt gaatccccgg tagtagaggg tccggtaggc ctgttcagtg
    59641 agtctctcga cggcctccca cagatgattt ccctcatagg gaccacctcc tgcaactata
    59701 atagctttgg gtggcatggt attgatctcc tctttggatt ttggcctttc atgtcgaaat
    59761 gcctaatctc tcgtgtagtc aggaaggcac aaagagcatg gactgaggtt tgaagcctta
    59821 gcttcttccc gtgtctggaa tctcgtggtg gcgtagcggg cgctggagca gccgggacgg
    59881 tgatatgttt tggaagaggc aagataatag ccctcctcgc atcgccccca gatccctctt
    59941 cgtgcctgct ttgcctggat ttcgagatcc tggtaatcct cccagtcgct cagcccgcgg
    60001 tacgtcatgg catagccgtg ctctacaagg agccggttta cacagaggtc tccaatgaag
    60061 agcccggcaa gtatccggtc cttgtcatcc cttgggctgc cggtcgtctt gagagtgatc
    60121 ctggttccct tgggacagag ccaaagaagg tggatggttg agatcctcgc aagaatgtgt
    60181 aaagtgtccg tattaacccc gagatattcg cactgttgcc gagcatgctc atcgcttccg
    60241 gtttcagggg catcgatccc gaggagcctt accttctcaa cgaccccgag agcggagagc
    60301 ctggcgcgga gggtatctcc atctatgatg ctgtagatct tcgcctcatg gctctcatgc
    60361 tcggtgacat tgaagctgat cgagattgag atgtgccgaa gctcgggatc atctgccgta
    60421 aagatgactc ggctctttcc catgatcagg cctcctcgtc tgaatctgag ccacccggct
    60481 caccttcgga gtgttctgtg gtctgtgcgc cgctttccgc gctgcgggtc attgactgag
    60541 tgagcttcgc atcattctgc ttcgcggctg atacgcactg ggatactgcc gagacggtat
    60601 tggtgatgga ttggttggcc tgtgcaattt gtgccacact cgaagaagtc gtggactggg
    60661 tgttgttctg ggtttctgtt tctgcactgg agtccaaggt atcgagcaag tcttgtagag
    60721 catcatctgc ctgctccagg atggctccac tttgctggat tgtggaaagt gcatctttat
    60781 cgctcatagt ttttcccctt tcatcgtgtt ggttttcacc cctggggaga ggtcctgctc
    60841 cgtgttggag gaagtcgtgc tcgtcaatgg aacggccgga ggtctccttg tagaagaggt
    60901 ggtttccgat ctggcatagg tactcgagct tcgatgccca ggaaggcctg cagcctgctg
    60961 cgtggtagtg tgtggcgccg cccgtcgggt cgtcttttag atgcccccgg atgacgagct
    61021 ctgctatggc tttacagaaa gcgagctcag cgcagtcgga agagagcttg agaatgtgcg
    61081 gaagatttgg atcgcttgcg ttaaagcaag agaactggaa aggtttgagg atgacatcgg
    61141 aaatgctcct gccgtaccag gattttgcct tcacccggtt tagcaccacg tgcgctatag
    61201 cgatcttccc ctcgaccttc tcacctcttc cttcacccca gatgcagcgg gcgagaagat
    61261 ccgcatccga gagcttcctc aactgttcat actgatcttt catggttcac tccgtttacg
    61321 acaggaatga aatcgtgaag tggtgtcgag taggggcggt aaggcttgag agcccaaccg
    61381 ccactttcat tactcgacta cccccatgta ctcgtggatc tgaagggcgt tgatgattga
    61441 gatggtggcg gcagtgacac ggtaggtagt cttgctgata atggagattt tggagacgag
    61501 cgtgagatgc tgaagtgctg ccacgagatc atcgaagttc atgacattgc cgccaggatc
    61561 caggaccgtg agattttctg cgattccgaa ctcctcgcca tctgcaacga tggtcctgtt
    61621 ttcaacagat atcgaatcaa ggcttttcag ctcgggagcc tcaggcggca gggaattcac
    61681 gttcgttccg attgcagcat cgtcagagcc gctggtttcg gagctttccg cggtctcttg
    61741 aactccttcc ggctcggaga tcggcgcttc ctccgcagga gcaggtgttt cagaagaggg
    61801 ctcgggagcg ggtgccggag atgcttcagg ttgaactccc ggctgagttt cctcagaagc
    61861 ttgctcggat tgggactgag gtggtggctc gggagcggat gccggatcga cagcgggtgc
    61921 aggtgcgccc tcggaatccg ggtcgggcga agggacagca ttgagtctcg cctcgacggc
    61981 tcttttgagc tttcccgcag gagtgaatga gatcttgatg tatcctgtct tcttggcctt
    62041 ggaggccttg aacctgccga aatcgataat cgaaagatca ccctcgctca agacgtgagt
    62101 gatggcggaa agaacgttat cgatcgttcg ctcggctgct ttctcggcat ctttgccgga
    62161 aaggacactg agcgggatga gcttcttgct gatgtcctgt ttcttgaagc ccggctcttt
    62221 gagaaaggct tcatcctccc tgggcttcct cctgtccttt ccaggctttc tactggtttt
    62281 tctcttcttt gccattgtct ctctccttct gcattgctct catttcgttc gattcggtcg
    62341 atgcgccttc aggactactg agcggtgaga gccttggagg ccttgaactt cacaaccgtt
    62401 ttggccggga tcatgatggt ctcacccgtc ctcggattgc gcccccgtct cgcctttctg
    62461 gtcgcacgct tccacgatcc gagcccaacg agttgcacat cctccgtctt gagggttacc
    62521 tggaagacat cgatcagggt tccgatgaaa gatgacgcct cagagatagg gcaccctctc
    62581 ttctccgcta ctgctcttgc cagatcgtct ttcgatgcca tggtgatacc tcctttgtcc
    62641 ggatcggtct ttgaaccgtg gtacgtatgc tgcgggcagg ctccgcccaa ctcgggtcag
    62701 agtccttttc cccgctcacc tgccgcgccc tccatcgctg aaaggccaaa tcttggatac
    62761 tcgatacgtg gcggcgcacg cagggtttcc attaggtgag ggactcaaca gccgaaaccg
    62821 agatcccctg ccccgaaagt ttgcccccgt gttcaggaag aacggggttt tcgtgatcct
    62881 cgtgccttct tcttcgtgca tttcatcagc gggttcaaaa attcgtcagg ccacatccag
    62941 gcacgcctgt cgattccggt gttgatctca agcttcgccg ccagttcacg agacgggcag
    63001 cgacgaccat ggagtatgtc gctcaggtgc ctcggagcga tctccaaggt cgaagccaaa
    63061 gccttttgcc atcctcgctt cgatgtgata gttttatact ctaccatacc atgccctatt
    63121 tgaaagattg acctattttt aaataataga gatcaccgtt gcaagcattt tttgttgaca
    63181 ataatagagc cagtagctac agtatccatg gaaggttgcg aaatctgtga ttaacctggc
    63241 tatggaattt ttttagacgt actatgtggg agggatgtat ggctggtgag gagaaggaga
    63301 acgaggttta tttccccgca accgactgct ttcgcgaggc actgaacaag cggctggacg
    63361 aggagggtca tggggctcag gcccgccttg ccaaggcgat cgagagggga acgaagcatc
    63421 tcaatgacat cgtccaggga cgaagacctg cgagcctgga tttccaggaa agggttgcaa
    63481 ggtttttgaa agttcccctg gacaggatgc ttgccgaggg aagagagctg ctcaaggaga
    63541 aggccaagcc ttttccgtat gcggaggcgg ctaaaaaaat caagactccg gagaaacgtg
    63601 ccgaatttat atgcgagaag gcggtgcagg atctcgaatt aggtgagagt ccgctcctgc
    63661 cgtctcagcc catcgttcgg gaggtcgccc gtaaggcatt gggcgatcaa tgggaagagt
    63721 acataagagg ggctatggaa gatgaggact tgtttgtcca cacgcaggac aagatcatgc
    63781 tggccattga agagttgagc aagaaaatac cttccaggaa gaaacgcaaa aggccttgac
    63841 atgattggct aaaccgtcct attttgcgca aagaaaaggg gctgcctttt cgtaaacata
    63901 attcgccaaa agcgaacctg gtagagcatt tgctacaagc aatgttttct gtttcggatc
    63961 cccaccccga acatgggatg atcaacgcag gctgtttcaa gcattttagg ggctaagcat
    64021 caatcataca aaacggcttg gagcaacccg cccgactgga ggaggagtag agatgagttg
    64081 gttgtggcag gagataacca aggggtgaat agatgccgat acagaacgaa gcggaaagaa
    64141 tcctcacaga agaggcttgc aggtgggttt cctctcccga cggacagcgc aagctgctgg
    64201 aggcaatcgc ccgctcccaa aagctgatcg atagcattac cgcggctcaa gaggttgatc
    64261 acaaagttct agccgaaccg atccagtaaa ttgacattgg ataagctcgc cgcaaagtgc
    64321 tcttaagccc acgttccaca gccaaactgc atccggtgcc gttacgggcc gtgacgtgat
    64381 agtcccaatc gtcggtttgg atttgcaggt gccgtgggtt gatggggaag tatatacaaa
    64441 tttcggctcg catcgggtcc tgggaggggg ttataaaata tggaggatat tcctcgtcta
    64501 gcgaacaacc ggtatgagat tcacccctac agcatagaaa aactgaactt tctccttgca
    64561 aagaaatcgg gggccgatcc gtccgtgata cggaataagc ctcactgtga ccggcttaaa
    64621 gtatatttca ataagatccc gacggaaacc atcatagttg aagagcacta tgtcgatcat
    64681 catttcctgg atgatttcac cggctactac gtcgtctgct tcaagcccta caagcgctgg
    64741 tgcacccgac tgcacttttt cggaaacaaa ttcggcaagg agactttcga agacatttta
    64801 cagaagaaga tcaaagggga tgaactacaa gagatctctg attcctacat tggttttatc
    64861 gttgtaaagc ccttgccgaa aacgatcttt ggaaaaacct gtctgaagac cctttcagaa
    64921 gacccgcctg gcagaaaata tcccatcaca cggcgctaca gggcgaatct ttttggatgg
    64981 aaccttgaag tgaaatccct ggctttccag gagcaggaca aggtagtggg cgcatgttcg
    65041 acgagcgctc tatggtctgt ccttcaaggg acgggagtga cgtttcacca tcacatccca
    65101 actccggtgg aaatcacgcg ctctgccacc cagtatgttc ctcccgcccc tcctgaaacg
    65161 aggcattttc caaacagcgg cctcacggtg cctcacatgg cgcaggcgat caaacaggta
    65221 aacctggtgc cgttttatgt gagcccgagg acagccgatt tccagtgcgt tgccgactac
    65281 ctcaaagcta ttgtctacgg ttatcttgaa ggacacatac cgatcttgat gacctttcac
    65341 ctcgtggact atcccgaagg agaaccgcca caacttcgca aaggcattca tgccgttgca
    65401 ataaccggat acctgcccga taaacacaag gcagttcctc tcaggcacaa agagggagaa
    65461 cccggctgcc tgctcaaggc atctcgcatc agcatgctct ttgtccacga tgatcagata
    65521 ggaccgttca ctcgtatgga attcagcgag gagggtcgcg aatacatcct gccgagagag
    65581 ctcgccgagg gatacgatta ttccctcagc acctcctggc gctgtgccgg cggaaaaccc
    65641 ggaaaggtcc gggcagtgcc tctggctctc atgatccctc tttactgtaa gattcggatc
    65701 gattacggca ccgtgctcaa agcagtagtg agccttgatg gcatcataga gatgttcagg
    65761 aacaccaatc gcacgactct ctcacaacaa ctcgaatggg acatctacct cactacgctc
    65821 aacgactaca agacgcagtt ggcacagagg gaggatctcg actggaatgt tcggaggcag
    65881 gctttgattc atggcatgcc tcgtttcata tggaaggcca ctgcctatga tggggagaaa
    65941 accgtcatcg atcttctgtt cgatgcaaca gatattgagc aggcatgcct cctcgcccat
    66001 gccgtgcctt acagcagcaa tgtgtatgag ctcctggagt acgtttccaa ggatcgaaag
    66061 ctttccaaaa tgataaagcc gacgatggcc tggaaagttc tcgagtggtt ttcaaatctc
    66121 aagaagaaga acaattccaa ggggacgttg aagaaataga tgcagcacct cagagatcag
    66181 cgacgacaca cacgattcgg caccatcttg tgcgaaggca gcttcggcgg ccttgatccg
    66241 tgtgtgaata cagaaaatgc ttatttttca tcaacttaag aggcaggggg ttttgccata
    66301 attttgccaa aaatgcccga agggcaggat aaacatgggt agaaaacggt agaaatggtg
    66361 aaaatcagcg gaagagaaga tattgccaat cgttggatct tcctatgtaa aatctacttt
    66421 ttcctactct tggttccctg ttgcttgcag tgaattacct acttctaatc cgtaggccgc
    66481 aggttcgaat cctgccaggc gtgccaataa aatcaagggg ttacgggaaa tcagccggag
    66541 tctctttttg cttttgtgca catcctgtgc acggattccg ggcgaaaagt cccatggtcc
    66601 ggtccccgca gctccctttt ctcccccata caaggcatgg tccccgcgcc gtctccggtg
    66661 ggtcccccca tgtcgcccgg aagcggtcaa acatccatac agctgaatat cggtgcccct
    66721 ttgcagaaag agtgaggaag ggtgaaaaag ggtgaagaga ggtgaaaagg acggaagaac
    66781 aaggagtggt gcggttataa cggagcgatc gctttgagag catctcattt tatgtgcaaa
    66841 tgattttgag gcaaaaaatg gggaaactga ggtggatttg catgaagaat gaggagataa
    66901 attagacgga ttttggggtg gggggggtg
//
LOCUS       DAOOVC010000004        64691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_246_length_64691_cov_6.182839, whole genome shotgun sequence.
ACCESSION   DAOOVC010000004 DAOOVC010000000
VERSION     DAOOVC010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 64691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64691
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_246_length_64691_cov_6.182839"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1115)
                     /gene="cas1"
                     /locus_tag="PLM79_00980"
     CDS             complement(63..1115)
                     /gene="cas1"
                     /locus_tag="PLM79_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOV84906.1"
                     /translation="MERMYVLEPGSTLRKSGRNLRLMKNEALVGEIPLENLEQLVLVG
                     FASLSGAVLDALVRYRVETVLLTPRGKFRARLLVDEHKHVQRRQAQYLRLHDPGTALA
                     VAGSLVRGKVRNSSRLLALRGSHFECRELLQAAAQLKGIADLVSLQTDLDAVRGLEGH
                     AANLYFKAFPLLVRAPGFSFQGRSKRPPLDPVNALLSFVYTLLTQEVLTAVKIAGLDP
                     YLGALHAVEYGRPSLACDLVEEWRNFLGDRLVLGLINRRVIAPDDFVVREVACTDAVD
                     EEDLKEKRPVEMKPRVARAFIQAYEKWMETRIRCPRTGEHTDYRGLIRRQVRHFSRFL
                     QGEDEAYEPFTWSRVQ"
     gene            complement(1420..3063)
                     /locus_tag="PLM79_00985"
     CDS             complement(1420..3063)
                     /locus_tag="PLM79_00985"
                     /inference="COORDINATES: protein motif:HMM:NF040561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type A1"
                     /protein_id="HOV84907.1"
                     /translation="MKAEPAQQEVVKKPGSSPDFGLLLRRLEEAALTDAGRDRLDRIL
                     RRETVWKRLPPEEAVRWARLAQVAGLCELSLEVLAWVNARHPENTDAWKQRIELLEIL
                     GKGTRRSSGTPTAAGPNEEATPGTPGPAGAPLSAPMDPAPVPPWEDGLEAPFAAMRER
                     EEVLDGYLRLFQGREDCFARQWADRKEGTQGYVPVRRPMTAADVQEHVNGLKTYGIYL
                     LQRDSRVRLAVVDADLAPRFRAGTLSSPDRELLAREKRYLLGRLPEIGREKKLPCLVE
                     FSGGKGFHFWYLFSEPVPAAAARRALQELVRHLAADLSCFTLEVFPKQDQLAGKGLGN
                     LVKLPLGIHRATGRPSFFLHVPDRSAPAQLAALRKAPVIASSRVMEVAGERKGAEVMV
                     HPRQEAWAARFPELALLGERCTALSQIFVSCRQARTLSVREEKVLLGTLGFLDRGKTL
                     VHHLVQELPEYNPHLVDFRLSRLRGKPLGCRKIHAILGLTGDLCPFERVPEYAHPLLH
                     WPQWKEEASANRAERIGNLREALEELKHAISVVERFVDP"
     gene            complement(3097..4530)
                     /locus_tag="PLM79_00990"
     CDS             complement(3097..4530)
                     /locus_tag="PLM79_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV84908.1"
                     /translation="MELKAVARIIQHPGKADRPVKTAPPNPAPSQDSRGSAQSVSAHG
                     DYFDRLAEGGIAQDRLLHERKRILGEVKVLRDENRWEDIFTLLHPAEDRAPELVAAGL
                     QAPLLAEAAFALDHLGRFDEALALGRACVEMDPENFHYHSGLAYTAYNSLYAAKTRRV
                     MLHPAERKARIELAHTHFEQARRLRPDGVTGLYRQGMLFKQIQNQKDKALPLFEAAVK
                     NWDALSAEDRKKRHQERKNFIKSLYQLSSCLLDAEKPRAAMEALARCLDEDASSQHLK
                     MVHKHFALGKIHFRLGQLDQARDALDFASTFADPADDDYVFELLARVHLARRDLARAR
                     QAVDRVPAQRRRPYFRWTESDVLVAEGDPDRARRILVETAERDRRSRHKALLRLVRID
                     YRRGDFESCLRWARDAQAFFWDQYGNSYADGLLWEGAALVRLGRLEEAAGAARRLEEA
                     HPWHPHLGRLKKALAEAAPGGSSPANP"
     gene            4963..6000
                     /gene="cas6"
                     /locus_tag="PLM79_00995"
     CDS             4963..6000
                     /gene="cas6"
                     /locus_tag="PLM79_00995"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOV84909.1"
                     /translation="MQTGNLLASAEAGSASLPEQGRKLSESLGSFRCLRLQVEVRARN
                     RVALPPYRGSTLRGAFGLALLRSACVLRQQKCPTCLLRRKCIYSYTFETPLWGENGEL
                     RRYAAAPHPFVLTLEVGREEVHEAGSEFEFAVTLVGRAVDFLPYYVHAFQRMGETGIG
                     KGRGRFEVLRVLEMDVGDEIAGPVYEGGVLRVPRTVLGLEDALAVAGELSGDGVTLDL
                     LTPLRLVYGGELCREPAFHVLVRNLLRRLHNLTLFHCDDGAPPPDGALIDLAEQVVPD
                     RRDIRWHDWERYSHRQERRMKLGGLLGKISYRGDLAPFLPLLVLGSWVNLGKNTSFGL
                     GRYRVKGADRK"
     gene            6031..8568
                     /gene="cas10"
                     /locus_tag="PLM79_01000"
     CDS             6031..8568
                     /gene="cas10"
                     /locus_tag="PLM79_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOV84910.1"
                     /translation="MSYTISETILGCLLHDVGKLLQRAYGRIEETGAKRYGLESTLCP
                     TKNGDSTHKHVLFTNAFFDLVRKENLFFPEGVNCLTVEHIASYHHKPGACNMPGAAWL
                     CTLGDHYSAGMDRRADEESAQRTSSMSGYRTTPLQCIFDELILDEAALGKPERHAYAL
                     EELNPEKPDALIPMAWPDNGTIRDLPNRYREVWAGFWAAFKALGRREPMLSFRLFEGV
                     LLGLLERFTWAIPSSTLDSPDISLYDHSRTTAAIAACLHRYHENRGELEDVKAIRNES
                     QPKFRFLAGDLSGIQETLFSLQTQGVRGVNKILRARSFMMSAICEAATLLILDALDLP
                     LSNIFQQAGGRFLILVPAQEDLEKLVEGFRQRIDQWLLERYTGSLALNLALSPPFSAA
                     GFRPAGLREVMSQTAQAVEEAKQRPLSTRSHGVLKREFPLDSSCSACGTRPAEIEDEG
                     GLRCLTCDNEIRVGKRLVRANMIVWGQGLPKRWQTTNVLGLEMGLLDKAPDESLEEVL
                     TVQRTNNDPAPFPWPVKVLANHVPLFRDDYAAHDPRYEWIKDEDTAQGAGEPKSFMHI
                     AAEALEAETAGGFRGRAFLALLKADVDRLGFLFSFGLRRNEIKDDRLTLSRLAQLSRM
                     MDLYFTGYLKGLLHREFPDTYTVYAGGDDLLLIGPWRQTIELSSRINDTFRSYTGHNP
                     NLTLSTGLTLLKPNHPVNRAAKEAEGFLDMAKDEGRNRICALIPKAITWDRYRERLRD
                     ADWIHDRMHDEAPVSTSFVYRVLELAGDAEAVTLRGDVRKAGWRARLAYLLARNIKGR
                     GREDTQHRIIEWLERLKLDDQFKLRGEHPNLFDWRLPLSIALYRNRS"
     gene            8587..9072
                     /gene="csm2"
                     /locus_tag="PLM79_01005"
     CDS             8587..9072
                     /gene="csm2"
                     /locus_tag="PLM79_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOV84911.1"
                     /translation="MAPQHHHQQGRSQQSGRGGQQSAGTDQLPVPQPLRYYSDDAKKQ
                     LRPELVDEEAHDSARRFSELKSTQMRRFYDEFKAIERKILSGGGLQEQEANFERDRAL
                     IMMFKAKAVYAERRRVSPRAFTEFIFNHMASIKDLRDFQAFLKVFEAVVAYHRFYAKD
                     N"
     gene            9088..9816
                     /gene="csm3"
                     /locus_tag="PLM79_01010"
     CDS             9088..9816
                     /gene="csm3"
                     /locus_tag="PLM79_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOV84912.1"
                     /translation="MRLLNSKTLTGHIRVITGLHIGAGKDAIEIGGVDSPIVKNLYSS
                     EPYIPGSSLKGKLRCLMEWATNRVRDDGKTWDGGNEDDPVCLAQDPVLRIFGTTNKSW
                     NAGPTRLVVRDCPINPQWRDEIIERGLPFTEEKFENNIDRIQGKAGVGIRKTERVPAG
                     ALFDLTMVYRIFDTGDQGKHDQECFKALLQVMRLLEQDALGGSGSRGYGRIEFDGLCV
                     DGKLFQDEFRKIAPQEILELRRST"
     gene            9818..10759
                     /locus_tag="PLM79_01015"
     CDS             9818..10759
                     /locus_tag="PLM79_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84913.1"
                     /translation="MPVYRIELTLKGSLGTDLLSGTVWGHLAWALRYLEGEAALMDWL
                     QDQVSRPWLLSSRMPVGMLPRPILKPVLRKTVRPSLKEMELEKRAKGLAYIPETVFQL
                     LRNEMSDEALSDVLKRGMGKWRIKESLPRKGINSHNTIDRLSGTTPDVGGLFFEAVFY
                     PGEDTKLQIFLHTPDPCLDRIEALFAFVGENGFGSNSSTGNGNFTCEVKEESTLFSAK
                     GNRAMSLSHGVITESMKDARYKQHVHFGKLGGDYAKGSYSPFKYPILMARPGATFQPE
                     GNGPFGKLLDAVHHDPELKKIRHHAFHLPLYYTEVQS"
     gene            10756..11925
                     /gene="csm5"
                     /locus_tag="PLM79_01020"
     CDS             10756..11925
                     /gene="csm5"
                     /locus_tag="PLM79_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOV84914.1"
                     /translation="MKAYRFRAQALTPVHVGSGNEINPLEFVLHENRLLRFSPAALIG
                     SLPPNELARYLHLLERADLRAIHSFFNDVDVGLAGLTTVDISPHFMREYEEKASNPNN
                     QFRVEMMPCNPHSSKRYLPGSGIKGAIRTALINHFADTDPHTRETVRDAVTRERDKRN
                     RARVLEEKAFDRKHSQTERDVLRLVHVEDACLPDGATRIDRAVNWNPNRKGAENIQMW
                     VERLKSVADGGTAPQFSVILHMDNTKMQHPPVKRSLGRTIDFDTILKACNHFYWGRMV
                     AEGEKFDGKNSGGKVWSALLDLFPKGKTPDGQIVSIDPAKPYWCYEQRKRILLRVGRY
                     SHFESLSVDGLREGYNAQARRPIRDMGSTRTRCLMDNGKPPMPFGWIMMTLESSD"
     gene            complement(12289..12981)
                     /locus_tag="PLM79_01025"
     CDS             complement(12289..12981)
                     /locus_tag="PLM79_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOV84915.1"
                     /translation="MAIKKDVDRHRHPGRYILTGSADVLTVPRLAGSPAGRMEVLTLW
                     PLSMGEIKGTTENVVDGFFEEEFPQRCRPDPDFDLGANVLAGGFQETLQRKAPRRRGA
                     WFESYLTTILGRDIRDLSKILNLAALPRLLALLAGRIASLQNQSELSRASGIPNATLT
                     RHMAVLQDTFLVRLLPAWSSSPGKRLVKTPMFMVDTGLACHLMGMDLAGFLRGGELAG
                     RVFENFVVLELF"
     gene            complement(13330..14673)
                     /gene="csx2"
                     /locus_tag="PLM79_01030"
     CDS             complement(13330..14673)
                     /gene="csx2"
                     /locus_tag="PLM79_01030"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02221 family CRISPR-associated protein"
                     /protein_id="HOV84916.1"
                     /translation="MANIYVSFLGTNDYLECIYCGDGLEPVGPVRFVQQATIKNNCST
                     WGADDRILIFTTEEAERKNWLDNGHRNKEGKILERKGLRCCIGKMELSVPHENVTIPE
                     GYSEDEIWQIFKRLNDCLCPGDNVVFDITHALRSIPLVAVVVLNYAKALKHVSLRGIY
                     YGAFEALGSYDQVKAMEVESRKVRILDLTALDQLMDWTVATHRFLESGDARMAGALAA
                     ASVRSILKETRGKDREADTIRKLGTELHRFSDMLSTCRGQEITAVAAALKEQVQQCRG
                     LDLLPPFRPLFDTIEKRLEKFAGDDLRQGLTAVRWCLDHGLIQQGFTFLEELLFSYVV
                     LGVGADPLDTTLRDVASQAFTLVNRKWDDTPEQWYSPAKDHPDTTRRMIGFIQAQGNL
                     CQEVEKVRSARNDLNHAGFCRNCIRARDGRRFGEKLRSSLDAVEKLFEGDPSHRA"
     gene            complement(14677..15069)
                     /gene="csx20"
                     /locus_tag="PLM79_01035"
     CDS             complement(14677..15069)
                     /gene="csx20"
                     /locus_tag="PLM79_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx20"
                     /protein_id="HOV84917.1"
                     /translation="MAKNLVLLFNHSLTPAQEAEARETLGVSNFLHPPESVRELWRNI
                     PPEAETLAGYLAPVEEWVKEAAKPGDAVLIQGDFGATCLMVRFAREIGLDPVYSTTER
                     RAVEEPQPDGSIRLIHHFRHKRFRRYGG"
     gene            15307..15771
                     /locus_tag="PLM79_01040"
     CDS             15307..15771
                     /locus_tag="PLM79_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84918.1"
                     /translation="MSTFSSRTAAIWESPYGPVPPDADLAVLGSGIKYLPPLLWKSRM
                     LFRGALDRIVKKLTGDFPHTLGRGKTTHPLFVLKSLPNPAHRVCPCSSKDWGARRVIR
                     KGCRLEYTGQITERDTFLVESCSFNLPMDSAFTNRLFFLGRVPEECIETRDS"
     gene            15768..16832
                     /locus_tag="PLM79_01045"
     CDS             15768..16832
                     /locus_tag="PLM79_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84919.1"
                     /translation="MTDYQGWRDWLAGPGCWPAMTEAARFVYRMATRVGLPSSLLPCG
                     NPWSLPREERDECFEELAREMWLFLRSMSAQEMKRIGADPTTHGNTRLLMQRIGQRFF
                     LHLKDQARTSPRDPERALYRRLRQVLSQEPTVAYRTTADGAFYSRDPGAPWPGTRKSL
                     HEEPYSSWESPLGRIGLRDLPRRSALLDVADFFWLQATTRLGEPSFLPVRELARFIAC
                     HYPGFSLPTPVSIHPGGTKADCLEGREVEVPSGEPGPDIQFMAQRLPALARQLVHGWD
                     LRQRSAFVLLVGDGVPLQEAARSLGYRNPSGVSYLARSVYDSLRDFCLLWPGLSPPDL
                     DPGLFEAFVLEVVTVCKKGT"
     gene            16894..18048
                     /locus_tag="PLM79_01050"
     CDS             16894..18048
                     /locus_tag="PLM79_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84920.1"
                     /translation="MCSYEPWQMKMAWQIALQARGCPPDSVLREGKADPRLQAHRDRC
                     PFCSEHGTVEAGDRTVETGLFEGTALAREMARRVEIDHHGEPLPGSICRISGALAGWG
                     PRERYYNPPLVLVLDHPRDMPGAARVAQVYDDPCLAGPGDVPLDHGLFAESWNTYALR
                     AEDLECAFGFVNETKLREVLDAEASDGPGPKQGTILEAFRRLEVEVAAFFALQALGDL
                     MDRREFGPLEEIAARFPHGESLKKALAEGEPHIRWPKDTTDPRAMLTHAQVAEDRLPM
                     AAAGQEEWITANMVVLDGETLRLEAVFCEIRHYSMQGNALVVGGKVPPSPAGRAEITA
                     SWVKEGLPPRDCDQSFLDEESGFFRLLFRDMTEAEFKSGRLLVLVAGLAK"
     gene            18075..20159
                     /locus_tag="PLM79_01055"
     CDS             18075..20159
                     /locus_tag="PLM79_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84921.1"
                     /translation="MWARRHCLHERISDLLYPPGFEFPATEEDLVGSWKAVEDALGPQ
                     RVRVLARGSWAGALRQRRSAPPWFFPALDRLSPWKDLDFPFSWAEAAEGRWSAFALAL
                     ARAGEGGVLWAVMGQLEGAIRGKRWPKWWEEAADVHAREGAAFALDMLERELSRSFFF
                     WPILPVPNPPAVWGRSLTLPVYLAGWALARNVENRSLVCTGNVLEDGSLGEAGHLERK
                     ARLAAIHGFKGLLCPRGSGGLSLGAEGIEILEADDLDHAGFLWELSAGGTTGGLQEDF
                     RSLRDPNRLAANVHLLSPGVLRWSGFDGPYAAALREISRSSTLIEDFVRNLERRVEDP
                     NTSQDSLDRLLVPVTREFLIDLREIHPLAALKLAQVRMATASHRGRIREAEEWSSTAT
                     ELTQAALRHAEGLHCKADLLNREFVHHRHNHYDFRMDLPEEVRKTLDTLRALHALLKQ
                     EDPDCTLPSLGKLYGTLAQNCGFCGPEHLEELCRYAELAREAFGNGRDPLYLADWRRQ
                     FCYVFYGLLDAGDPAGAEEILSLYLGTEPRSLTDGRIRVLTPYEHAAMARFLAETGAD
                     VPEYRRWMSIHLGRRPSRHPWPLWLMNAGLCREDPAHRREAFTESALACLRQGPTAQA
                     MGLMPLQLLWKEGLQPHDWVRDGTRQILQEIRHSFGGQTHFSRLLEYSDWEEALFEVE
                     LQKPRLFPFTYR"
     gene            complement(20146..20631)
                     /locus_tag="PLM79_01060"
     CDS             complement(20146..20631)
                     /locus_tag="PLM79_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV84922.1"
                     /translation="MFTAHEVFDLAVQIEENGERFYRDGMEKVDDSAVRSLLEKLADE
                     EVRHRETFYRMKRELGRQDEDLWADRISGAVLQSFIEDRAFSLEEADFASVRDEGTLL
                     QIAVGFEKDSITFYEILLSFSPSAETAELIRAVIAEEHRHIGLLEEMAGELAAKPLTD
                     K"
     gene            20901..21224
                     /locus_tag="PLM79_01065"
     CDS             20901..21224
                     /locus_tag="PLM79_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV84923.1"
                     /translation="MKTGNITALVPRDRDREVFEELVRTDRVTIERIISHGQASPEGF
                     WYNQERAEWVLVLKGSAGLRFEGEDAVMVLREGDWIHIPARRKHRVEWTSKTEATLWL
                     AVHHA"
     gene            21575..22657
                     /locus_tag="PLM79_01070"
     CDS             21575..22657
                     /locus_tag="PLM79_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOV84924.1"
                     /translation="MDRKDRAVELLTELSGAHGAPGCENEVRRIVRRYFPTAHTDPMG
                     NIFVEKEGSASTPRILLTAHMDEVGLMVQAVLRSGLIKFVPLGGWWAHTLLAQRMRIR
                     TGDGRELPGVIGAKPPHFLSESERDKVVKIEDMFLDVGARDDEEVRRLGIRIGDPIVP
                     DTSLTLMGNPDFLLSKAFDNRCGMALMIQVLEELRQVPHPNTLWAAGTVQEEVGVRGA
                     RTAVYGINPDAAIVLEGCPADDLPGTPEDERQGALGKGVQIRRMDPSAMMNRAFVDYA
                     LETARSRDIPHQEAVRRSGGTDARAVHLHGRGVPTLVLGVPARYIHTQHSIVCVQDYL
                     SALQLALELIGRLDEDRVCRFTQFDD"
     gene            22830..23138
                     /locus_tag="PLM79_01075"
     CDS             22830..23138
                     /locus_tag="PLM79_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84925.1"
                     /translation="MRNGLKCLLWIFSLCLVVSCAHKEDSSRKVSQQPTPAAPAAAPA
                     AKTATPAAVPSPGSAPAIQLAETNHNFGNMSEDRQYEHEFKVKNTGGGVLEIKKVLPG
                     "
     gene            24108..24398
                     /locus_tag="PLM79_01080"
     CDS             24108..24398
                     /locus_tag="PLM79_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84926.1"
                     /translation="MDIILGTNPVKGSHPRTTASGGGTTVEARILYQRPCRKSRGTPP
                     SGQRERRHRRSLADPPNGRVVFLLVPEGNRLPRDLSNANYRVFLRLVPGSNP"
     gene            complement(24710..25696)
                     /locus_tag="PLM79_01085"
     CDS             complement(24710..25696)
                     /locus_tag="PLM79_01085"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV84927.1"
                     /translation="MDREPKETSPGGFFGVSPYMIFPSAYGNFSIFLKFGNHHILYAK
                     DGEAVTERHKAILYENGVEEVYIRTDDKPQFDDYVERNLSRILLDDNIPLPVRSRIFY
                     SCAVNVLERALEAKLSLPLTSDRHRKLVKLVESSVSFLCRENVFKTLGSLMSHDRKVY
                     THSTNVFLYTLAMLETYSLPEEEKVHIGLGALLHDIGKSQIPAKILNKPGRLNAHEWE
                     ILKTHPVKGLALCSAVPLDQSTIHCILFHHEQCDGRGYPTGLTGANIPLPVKVVNVAN
                     RYDLATSPGPRDHPLTPFQGLTKMKEEMQEEIDMDVFRRLVMVLSGAGLVQP"
     gene            complement(25737..26591)
                     /locus_tag="PLM79_01090"
     CDS             complement(25737..26591)
                     /locus_tag="PLM79_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOV84928.1"
                     /translation="MSFDSLLLVLLSALLHTVWNLMIKRVEEKHLFTWWALVAGSVLY
                     LPVLLWHIPFPPKIWAFALTSAVLETLYFLALLRAYERGDFSLVYPIARGAAPALLAL
                     WANLFLKERPSPAGLGGLVLLIAGLLVVGGAGWWSRRKGTSRSQDLVSALAAALCISA
                     FSVIDAAAVRYLFPGAYTVLVLGIMSLLITPVIVVHYGAKPALAVLRAHFLRVVVVGV
                     LVFGAYMLVLQACLSARVSYVGAIREISIVLAALVGWFQLGEQFGPVRTAGAVLIFLG
                     ILAIGIAG"
     gene            27335..27922
                     /locus_tag="PLM79_01095"
     CDS             27335..27922
                     /locus_tag="PLM79_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     cytochrome b subunit"
                     /protein_id="HOV84929.1"
                     /translation="MLSGVGLIIFMWSHMLLVASVNLGAGVMNAIADFLEKYYLAQTG
                     GPAIFALFLVHFVLAARKLPFRMKEQKEIWEHSKRFNHTDTWLWMVQAGTAMIILIMG
                     SIHMWTVLTDLPIMAAKSAHRIQGGWWLLFYLLLLPMIELHTSIGFYRIGVKWGFITR
                     ADRKFWKRIENRVTLTMVGIGLVTLINFYFIQKAM"
     gene            27932..29791
                     /locus_tag="PLM79_01100"
     CDS             27932..29791
                     /locus_tag="PLM79_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase flavoprotein subunit"
                     /protein_id="HOV84930.1"
                     /translation="MDTFYTDLLCVGAGLAGERAAIAAASQGYETICLSLVPPRRSHS
                     SAAQGGMQASLGNCIMGAGDNPDVHFTDTVKGSDWGCDQEVARLFAETAPIAVREAAF
                     WGVPWNRVVAGKGTYFKGGKQYEKVELKEKEHLITARDFGGTAKWRTCYTSDGTGHTL
                     LFAMDNEVVKLGVTVHDRVEAIAIIHDGETCMGVVARCLKTGKLRVYLAKATIIATGG
                     YGRIYQETTNAVINDGSGAAIALNTGVVPIGNPEAVQFHPTGIVPTNILVTEGCRGDG
                     GTLKDVNGERFMDKYEPAKQELASRDVVSRWMMHHIRLGKGVPSPYGPHLWLDIRHLG
                     EKHIKTKLRDVEEICNEFLGVDPITQMIPVRPAQHYSMGGVRTNKDGAAYGLKGLFAA
                     GEAACWDMHGFNRLGGNSLAETIVAGKIVGEKIAEFLQGYEVNFRTDVVQDCVKKEQE
                     RIDNIIKGKDGKENAYALRNEMQAIMFEKVGIFRNGSDLKAAVEGLQKVYDRANHVGL
                     RSGGVGANPELAFALKLKGMVRLALCVAYGAYKREESRGAHAREDFPERNDRDWLKRT
                     LATWKEGDDLPTLNYEIPSKVWELPPGDRGYGSAPIIPSEDPAVIGGKKPENK"
     gene            29811..30563
                     /locus_tag="PLM79_01105"
     CDS             29811..30563
                     /locus_tag="PLM79_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur subunit"
                     /protein_id="HOV84931.1"
                     /translation="MGRPLKFNIFRYNPMDPGSVPHMEVFHLEETDAMTLFVALNRLR
                     EEQDPTLMFDFVCRAGICGSCAMMINGKPDLACHTKTRDLPQEITLMPMPVFKLIGDL
                     SVDTGSWFRGMNEKVQSWIHTQKVFDPKAQEEPMTNERAEEIYELERCIECGCCVAAC
                     GKANMRPEFMGATAFNRVARFMLDPRDERSDAEYYEVVGTDDGIFGCLGLLGCEDVCP
                     KKIPLQDQLGILRRKMGFSSIQHLFGKVFARK"
     gene            complement(30702..32372)
                     /locus_tag="PLM79_01110"
     CDS             complement(30702..32372)
                     /locus_tag="PLM79_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOV84932.1"
                     /translation="MIEWKWEIAAQQQPCSVEDVVRILLENRGAGPSFLSSELADLKA
                     YLAVKGLEEGADLMARRLREDRKVVLVADYDCDGITSAAQMSLFLREIGYHNFSVVLP
                     DREEGYGIPGRAIPDHPDAGLFVVMDCGTSEREKIERIRGLGADCLVLDHHEVPREGA
                     APATVLVNPKQPGCPSPFKEFCSSGLTLLFLTRLRKALAGGFPRPRLGGKYLELAAIG
                     TIADLVPLVEGNRIIARSGMESLNRGPHGPIRAILESAGLDRKPLSAGHVGYYIGPRI
                     NAAGRVSHARIAYDLLVSETPEEMGSLAGELNRLNARRQQQEEEILEEIRRRLLWADE
                     SRKTLVLGDAGWPVGVVGIAASRIQQEWKHVPTILVSIDEKNGVARGSGRSIPGFDLH
                     RALMECRDLLQRWGGHKAAAGVTLDPANLDAFADRFEEVARRSDPEIFVPRKRVDMRL
                     DLGLVSGELVAALRGFEPHGLGNPSPLFAAAGTRVAVQRAFGKEGSHLRLVLNGGIEG
                     VFWRGAASFAAGRWGSLEDADVIFEVEWDAYRNKPALVVKDVGRFFER"
     gene            32992..33555
                     /locus_tag="PLM79_01115"
     CDS             32992..33555
                     /locus_tag="PLM79_01115"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV84933.1"
                     /translation="MDTFTYLKNFIRDRNVASITPTSSFCVRKVCNKINFDRSELIVE
                     YGPGTGVFTDYLLRRMRNDARLILIERNKNFNSILEKNFHDPRVTIVNGSAENVLDAL
                     NNSKETKADCILSGIPFMLLPRELKHRILANTHEALKKGGKFLVYQTCFQTENHLKVH
                     LDRYFPVVRTKFEMLNIPPLRVYEAIK"
     gene            33539..34585
                     /locus_tag="PLM79_01120"
     CDS             33539..34585
                     /locus_tag="PLM79_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HOV84934.1"
                     /translation="MKPSSSDSPGWKERDIENFQFRRIHPSLFMGTASDRYSGWVGQI
                     YTRELYEGRIRARTHRVGPNSFREEVLPVESVDEYFQHFGVLEIDYTFYDTLLCARGN
                     PTATHRILGEYRQRLSGEDALVLKVPRLIFARKIMQAGKLLPNPLYLDPEAFVRRFYE
                     PACRLLGNHLRGMVFEQEYQRASDRASVDRFIEELDNFFRSIPRDDRYHIEVRTPRYL
                     VSPVFSLFLTHGIGQVLSHWTWLPPLSKQWDLSGRRGSNRGGQVVFRLMTPRGMRYEQ
                     AYARAQPFDKLVPGMLQPDMIEDTVRIIRDGLDRGLRVHVIVNNRAGGNAPRIARRIA
                     RLFLAREKERTPEA"
     gene            34791..35411
                     /locus_tag="PLM79_01125"
     CDS             34791..35411
                     /locus_tag="PLM79_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84935.1"
                     /translation="MKSVERLLICAVVAWTLGFHLEPALADEVSDQIKEALQQYERGN
                     LSEAANSLRFAVGQIQQKLSGDLKDVFPPPLSGWSAEETTGDFGAASFLGGGISASRH
                     YFVRDSDRAVDIEIVTDSPLLQSVLMFYSNPAFYANQPDAKLVKIQNRKAVQKYSAQD
                     KEGEINLVIGSRMLVSIKAMGLDKPDDMVAYANAIRYDALERFLEK"
     gene            35617..37905
                     /locus_tag="PLM79_01130"
     CDS             35617..37905
                     /locus_tag="PLM79_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOV84936.1"
                     /translation="MARTAICSEDPNDLLRTSLEYQTRLNQIYKLLHEAHNFMDVLPD
                     LEKDLLSLLRAERLTIYQKGRGDREIVSRYKTGREIKEIRLPLSTTSIAGYVALTQQA
                     LRIDDVYDSKALASIHANLTFDSSFDQRSGFRTRSMIVLPIKFGDVLLGVLQIINRIG
                     GPFTDQDLVHAREIARVIGQKFRYDLGGTKGPYDYLVQTKRITPEKLEECKKVAAAQK
                     TTLTFVLMNEMKIPAEEIGASLELYYQVPYMKYDPDVELPKALLKRLNEAYLRSHLWV
                     PVGVDQEKVIVLIDNPTDTNRIMEIQKVLNARNYEFRVGLPEDILKFLGVGLEIPEPE
                     SVNLEDLVGRLEDEAAVIADEQDIALASTVSENEATVVQIVNRLVLDAERLGASDIHI
                     EPARGKVPATVRMRVDGVCRPVLKIPASHIRAVVARIKVVSRLDIAERRKPQDGKFMV
                     KLRGKNLELRVATIPTVHGEGAVLRLLHSGGALPFDNLNLSERNARETKELVSHPHGI
                     FLVVGPTGSGKTTTLHAILGHINTPERKIWTAEDPVEITQEGLQQVQVEPKIGFDFAA
                     ALRAFLRADPDVILIGEIRDQETAHSAIEASLTGHLVFSTLHTNSAPETVTRLLDLGL
                     DPMNFADALLGVLAQRLSRTLCSFCRKPHPASNEEVHQLMVSYGQEMFPELGVNPSEM
                     ILQGPAGCDRCGGTGYRGRIGIHELLVATNAMKVVIARKSGVTEIRNLATAEGMRTLM
                     QDGIAKIVRGFTDFSQLRRVVA"
     gene            complement(38824..39363)
                     /locus_tag="PLM79_01135"
     CDS             complement(38824..39363)
                     /locus_tag="PLM79_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HOV84937.1"
                     /translation="MEIAADIVEAQASVGQMTADQIELALLKTFGALQKMQKAEEEGV
                     LLDQAKALEEPSVKEKAPEKMEAKDSIQEDKIICMECGAEMRQLTTKHLGSHDLDPRE
                     YKKKWGFSLKQPLSAKSLTRARSSAAKKRGLPEALVRFQQERKLKKSQSSSEEPASGE
                     EGAGDKRTRTIRRPKNKKE"
     gene            39968..40360
                     /locus_tag="PLM79_01140"
     CDS             39968..40360
                     /locus_tag="PLM79_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84938.1"
                     /translation="MWKRTVFGTALLCLLSAACPLVFAQNFAGTYVRIGDSKEYLTLS
                     PDGTFFLKQRKKPSDLANPFQTITGHYQIQGTEIILRLPGGGEATGKIQENIFEDSDG
                     KKWAKDKSKIPLPQDTDRRPTEGRGQRY"
     gene            complement(40243..41247)
                     /locus_tag="PLM79_01145"
     CDS             complement(40243..41247)
                     /locus_tag="PLM79_01145"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOV84939.1"
                     /translation="MCADAFRRNRFPTRPPTVKCRAILPRYRTSPTEKRPKKPSEEAR
                     SGSNSSSNTLRPGLLHTQEEERQRIAVELHDSIGQSLVAVKFSIENVLRLLKRDGVEA
                     AELPDPLVPMVRGPIEEVRNIYTGLGPSVLDDLGIGATIGWFCGEFRNTHLTIAVEER
                     FSVEEAAISVSLKIVIFRVIREAMNTIARSAKVSRVSVVLEKDEGALTLTITDDERRP
                     DPQGGLGTPGGDPGVGLRSIQERVELSGGSFRVESAETQGPGFTLPGPPSRRPPADRP
                     EEEPMGRRLLAHELFRGPGNQYLWPRPSVGRLSVSWGRGILLLSLAHFFPSESSKMFS
                     "
     gene            complement(41219..41443)
                     /locus_tag="PLM79_01150"
     CDS             complement(41219..41443)
                     /locus_tag="PLM79_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV84940.1"
                     /translation="MNRDQRCRVVNKDYERWFGIPRSRIAGMSVKELLGAGPYGLVME
                     HVTAVLRGEAVSFEGEMHPAGGVRRCFPAE"
     gene            complement(41705..42391)
                     /locus_tag="PLM79_01155"
     CDS             complement(41705..42391)
                     /locus_tag="PLM79_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV84941.1"
                     /translation="MFIDKSMTRNVVAVDPECSVIEAKGIMTQNRIRHLPVVNADNYL
                     IGIVTDRDIRSALPSPILHEGESAGMEERVRELRVKDIMTRNPITISPAHTLEDALLL
                     IQRTRVGAFPVVDGQGKLRGILSIRDLMRAFINVLGIEEPGTLLCILVEDKLGQMKRI
                     VDAISEERISFGSILVARHWEEGKRAVFPYLLTTNVTRVKKKLEALGFTLLNPMQWHL
                     DQLPKKEEGA"
     gene            complement(42528..43478)
                     /locus_tag="PLM79_01160"
     CDS             complement(42528..43478)
                     /locus_tag="PLM79_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOV84942.1"
                     /translation="MKRTGFLYDERYLRHLTGPCHPEAAERLESIYRGIQQAGLLPKL
                     TLVEARPADLKWVETVHSREHIERLRDACLNGVSEFDHPDNQMCKDTFETALLAVGGV
                     LNAVRLVMEKRLDNAFCAVRPPGHHAEADKAMGFCYFNNVAIAARYLQKQWGVPRIGI
                     VDFDVHHGNGTQKAFETERTVFYYSIHEHPSFAYPGTGREFERGTDFGYGYTLNSTVL
                     PGQGDEEYRELIQRDLIPAFDRYRPLVILLSAGFDAHVEDDMSGVNLTTEGFSWLMET
                     MVGLAEKYAEGRLISILEGGYSLDLLPELVKNHVEILLGS"
     gene            43886..44509
                     /locus_tag="PLM79_01165"
     CDS             43886..44509
                     /locus_tag="PLM79_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOV84943.1"
                     /translation="MARLLHIQASPMGDLSFSNRLAAAFLGSYRAAHPNDSIEQLDLW
                     REDLPAFDFTAASGKYKVLRGLDHSPEEARARQRVVELTEHLKSADKVVVSSGMWNFS
                     IPYRLKQFIDNIVQPGLSFSYDPVKGYSGLITGKPLQILLASGSEYPAGSDAAAMDFQ
                     KPYLEMIFRFIGFEDIRTLRVEGTLSAQAEANLVKGMDAAVEAGKNF"
     gene            44664..45629
                     /locus_tag="PLM79_01170"
     CDS             44664..45629
                     /locus_tag="PLM79_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV84944.1"
                     /translation="MKVRFWGSRGSLPAPLTAEAVRKKILRALRDSRNLSLDNDEDLA
                     RFVEAQLPFSVRGTYGGNTSCVEIAGGDEAVLCDAGTGIRVFGNHVLRESANQGRKPP
                     RVFHLFMSHLHWDHLQGFPFFVPAYIPGNAIHIYGFHERLKEAFSNQQQGLHFPMPLE
                     NLLSDIRFHVLETDKDYAIAGFNVRGVKQNHPGGSFGYRFERGGKVLVYSTDSEHKAD
                     METDGYAFLSFIQDADLLIFDAQYSFGEQVSNKEDWGHSSNIIAVELSVKAGVKHLCL
                     FHHDPVLDDDALDRFLTDTREYLDLYAGSHPLRIDLAHDGLTIDL"
     gene            46013..47365
                     /locus_tag="PLM79_01175"
     CDS             46013..47365
                     /locus_tag="PLM79_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84945.1"
                     /translation="MKRLPGSLVAAVVLLVPLLAGWVPAASAENGPPLSQPLVREGDL
                     AMDLALDLGLSVESEAEAESKLASMGIEPRNGWISDYPVTPDILSEIHDAVGDAADSG
                     KLGMSKGEALALLTDISIDYGLSVRTTSDGSPPPVREAAVDPYYNDPSLINDYYTEYG
                     PAVVTYYTPPWDYYSLYSWVPYPFWWSGIGFSGYFILNDFHRRVVVVKKRVGHPKHWR
                     HKGRFEEGKVRTVSNRVVDRNTGITARLDPVTRTIDHGGRSVRNISNPRSRAGLESGD
                     GRRDSAPVPEGSLGRPSRGNAGARGTAIPDRWNRPASKESEAGRELRPTRRPNPSGNR
                     ERLRSMRPKGPSFAPNGRRSASSGSFRQGFAPRFDRRSWSGNGNRAFGTLQYRSGSFR
                     GSGGGGNIGPSIGRGFSGGNSRIGGGGGFSGAGRGGFLGGSRGGFSGGHGRSGGGGRR
                     "
     gene            47921..49969
                     /locus_tag="PLM79_01180"
     CDS             47921..49969
                     /locus_tag="PLM79_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84946.1"
                     /translation="MTSIHRNVGLRSVWVVLVLFGTIALPALSQPLHAQTWGKLYHGP
                     WTEWYTGTTFYGALRTSDGGYLLTGTSTERGVILKVSNDGTLQWSKLVKGNGYQRDTS
                     MNGAVEAPNGDFIVVGQAYTDTTMYPEAGAGSWDNYIIRLTSTGSKVWEKFYARKNVG
                     EVPDTNEYARSIYVDCPEGGAPCRYVVTGHVVYHCVPGCPYYGYRASQTMVIDEDGEV
                     QDDFLMGGPSSTGDGAVQTVYTGDGNYLVLTRLWHSYGGYTIGLQKYGPTGSLIWSRA
                     YYEAYRQEDGEHLLTITPGAVVETENGYVLAGFSYDNVTQDPPLGTYYHFQVDKTTGS
                     TVAWNKKYRVWENWWSYNAGSLVTTPDGGLLAKVEDIITTENDRIFKTSQNDGTKLWV
                     NEYGANLGTITPGAADLAITRSAFDGLLASLSINGLMDESCKALPISDKTDEAVLYPI
                     LRTDVFDSFGPSDCNSGIGKVAVYEADRIEPGEYQQVNACTPHGEVTADSNGLFGKVN
                     VGSPGTDRKVYLSNSNGLSNLYVNKVLLSNTRDFSIVAGSDTCTGKTLYPVNAGTPTG
                     CEVTVRFAPVTYGAKQGLVKFDSNDETLLQQVLKGTGVGGAVKVIISPKAARNAGARW
                     RVDGGLWKKNGDLVQLSTGSHEISFRKVKGWRKPDNKIVKIVNGETLQIRAEYRKVAR
                     "
     gene            50520..50702
                     /locus_tag="PLM79_01185"
     CDS             50520..50702
                     /locus_tag="PLM79_01185"
                     /inference="COORDINATES: protein motif:HMM:NF023665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HOV84947.1"
                     /translation="MPKDKPKRGPYANERVNPHEPHEIKYWTKKFNCTDMQLKHAVRA
                     VGTSADAVRHFLQGKK"
     gene            complement(50730..51257)
                     /locus_tag="PLM79_01190"
     CDS             complement(50730..51257)
                     /locus_tag="PLM79_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84948.1"
                     /translation="MIPVFAALHFAFSHADPVRYLWATLGLLSLTLPAAARLLFRKRF
                     VDGLCLGIAILTSVHIILYTPNVLYMTKKERCYLTLAQVVSREVGDHPLYLYRPKDHV
                     RGSIPFYRDRPVREINRPEEVAGVLSGPGKKFLLLSKTSMGEIRREGAWRQEYLAQFL
                     PDVAPDVYVLVSNGN"
     gene            complement(51287..52405)
                     /locus_tag="PLM79_01195"
     CDS             complement(51287..52405)
                     /locus_tag="PLM79_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOV84949.1"
                     /translation="MRLRVFRAGTGISILLAVVVLTAMSTGVDYFCLWGPDEPREAEI
                     ARETLEDGHWITPHLCGLPFLEKPPLYYNLVALSYALNGKVTSTSARVVSVLMGLVML
                     AATLVLVYRWKGLEAAWLATFVLMTMPKFWRYSHWILLDIAVGAFCSLAIVSFALWRF
                     WPPRTIPRQSLLYFLAFFSACAFLTKGTIAVFHVVVVVGAFCVVERRGEFFRSAFRPA
                     VTGTFLVPVALWVFLFYRDGGLGYLHEHFVNNILGRFLHIPFALQEVRFHHTDLGNKE
                     SWSFYLEVLPEILGPWLLFLPFALFEMAKEMRSGFGETRKFFLFLLIWAFVPPILLSI
                     PAIKERSYILPSYTAYAVMVAYTICTRNSSMENRWREG"
     gene            53136..53987
                     /locus_tag="PLM79_01200"
     CDS             53136..53987
                     /locus_tag="PLM79_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV84950.1"
                     /translation="MKRKCAFAGLIFALMLMTLLGAGRAADRKVVRVTSPGSMFGRMH
                     TISALFSRDQPMIQIDFVRSADVDTSFSALIERSTDIALTTRRITPTEDQLAKSQGME
                     LTGRVIGHGGIVLITAPGNPIDGLTLDQVRKAFTGECTNWRELGGSDGPITVVRVGEN
                     YPGTNFFMQEDLFGGRPFAANAVVVPEFNNVLRKVAQSAGSLGFVRIRDAFESPIPFE
                     MAVKVLKVRKDAASPAIQPSRATIADSSYPILRPYYVYYESKADTSIVKYVEFLEKKG
                     WGQQNLL"
     gene            54048..55028
                     /locus_tag="PLM79_01205"
     CDS             54048..55028
                     /locus_tag="PLM79_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOV84951.1"
                     /translation="MAVITISRQPGAFGEEVAARVAGVLGFLLVDRARLARFRQEMGL
                     REDDPTNAQKGNGGEDRSAGPEDETCARLLPDILAQLAEEHDLVVLGRGGQGLFRNRP
                     GTLHVRIIASRAFRIRQVRRRRGLSSREARRSLTELETRRAAYVRSLYRMNWMDPALY
                     DLTLRMDRLSIEEAVNLIATAAETMKLRQVPRRLIVEDLLPETEERKNGDGFANDTER
                     EFARFLEFYRIPYEYEPRTFPLEVDAGGRVLEAFTPDFYLPEQDLFIELTTMKQRLVT
                     RKNRKIRKLRRLHPEVNIRIFYQRDFHNLLAKFGLLWGTSPGNADTPDGQ"
     gene            55079..55645
                     /gene="hpt"
                     /locus_tag="PLM79_01210"
     CDS             55079..55645
                     /gene="hpt"
                     /locus_tag="PLM79_01210"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOV84952.1"
                     /translation="MTTRFDQHITEVLIPQEAIARRVAAMGEEVSAAFENSCPLLVGV
                     LKGSALFMADLVRHMKIPLEMDFMAVASYADQEQRVSSGAARITKDLDNVIEGRDVLL
                     VEGIVDTGMTVGYLLRSLEARQPRSIRLCTLLDKPARRIVQVPIAFRGFEIPDRFVVG
                     YGLDFRRLYRNLPYIGVLSGEATGLNRS"
     gene            56690..57898
                     /locus_tag="PLM79_01215"
     CDS             56690..57898
                     /locus_tag="PLM79_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV84953.1"
                     /translation="MQADAAQNGDPIRVGAPIPRASNYGQNGERGMILAVEEINESGG
                     VKVGDRRRPFRLEIIDTRDEEPGVPTSEVLLAIEKLILRSKVDLVAGGPCMSECGMAA
                     LDLFARYRKLDLVSIGCYTPSWDEKVSRDRHKYRFSFRESGSVRWYVREALDLLRRMK
                     ERFGFETLFVSIDDSLMCRRASEIVETLAIKDGWKIVGKDVHPIASTDYSAALTECRK
                     SGAQILFLWAYSPETSILMKQWATMEVPALPVGFIGAAEDPGFWEATDGQCAYSIVTL
                     SETGNVPTRATPKAMEFHHAFRRRWNVPPRSTGCVSAYEAMYVLKDAIERAGSLKEEA
                     LISALEKTWLEAVRGTIRFDENHQVVYGYDPRTSVLGCWVQWQDGERVQVFPDAAAMG
                     EIRLPPWMKR"
     gene            57954..58841
                     /locus_tag="PLM79_01220"
     CDS             57954..58841
                     /locus_tag="PLM79_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV84954.1"
                     /translation="MESVLVYGTINSIIFALNAIGFSLVYGVSRVPNFAHGSIYVLAG
                     FVAWSLVNDLGLPYPLAVAIALAFSAVIGWGIHRLVLRRLRGLPTSEIIASFAVGLII
                     LEGLRLQGIGGFKGFIGPNYVLKPFVDGKIEVLGVFVDSQRLLVVGVGLVLAPVLWAF
                     THHTRLGLSLRAVAQDEQAAMMLGVHSDRSALIAMVLGSALAGLAAVLVLPLGNVTCE
                     VGYQVLIYALAVCIVGGLGNWAGTVLASFLLGFAMKVSTHWFGAVWESVVLVGTIILV
                     LLVKPSGLLGKQKELEERV"
     gene            58841..59851
                     /locus_tag="PLM79_01225"
     CDS             58841..59851
                     /locus_tag="PLM79_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV84955.1"
                     /translation="MSTRKLRKRRVDRGIHVRTRGLYALSSYREMAYLLAPRLFLLVG
                     LLGLPPALDLYGQRVLCAAAVFAMPALAFDFLAEYVGLVCLGGALFIGFGGYAAGFLN
                     ARLGLSPVLTIPLGTLAGACLSTLVILPCLRLRGVYFAMVTFMIPLFARHVIVALGLL
                     GSTEGITGLDTLPDPLIEIYVILAAVLASLFALRRLVNEDLGLILRGIKENDQAVAAS
                     GISILRYRVLAVFIASCIGCFAGAYLAHLYGWLGLSLFAMDFSILPIAAAVMGGTGTL
                     AGPVVGAFLLTSLSEALRGFGQLRVVLYCLVLLAFILYKPEGLLKVLERKYQQFERRE
                     EV"
     gene            59854..60612
                     /locus_tag="PLM79_01230"
     CDS             59854..60612
                     /locus_tag="PLM79_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV84956.1"
                     /translation="MAAGVLLEARGVTKFFGGVRAVRDLDFSLGQGEVLGVIGPNGAG
                     KTTLVNILTGFVRPDRGRVVFRGKDITGLPPHKIANRGLARTFQGMRPYPGLPTYKNL
                     VVPLNSPRVRKTAAGRFGDLDSVALDLLEEVGFERDSSVPYKTADSLPLGYQKRLELA
                     RCIALRPEVILCDEVFSGLSMAEISSMLPLLEKLLSRGIALILVEHRLRELFRITRRV
                     LVMRFGEKIAEGPPREVLGRPEVTAAFIGRGSPP"
     gene            60609..61394
                     /locus_tag="PLM79_01235"
     CDS             60609..61394
                     /locus_tag="PLM79_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV84957.1"
                     /translation="MNLLEVHNLMVFYENSVGVNNVGIQCREGRITGVFGSNAAGKTS
                     LMYAISGIIRDTARKEMMRGGMRIAVLGDVFFGGENVTDLEASRRARKGLVLCPERRR
                     IFKESTVIENLRMGGFLAGRTEARRTLEYVFHLFPDLARLKKREAGFLSGGEQQMLAI
                     GRALMAQPRLLLLDEPLLGLSPAMEDALISAVADINRETGLSTLIAEQYARPIFPILS
                     YGYVLENGGVTAEGTSESLMENEDVRAAYFGMTDEEDEGSWPS"
     gene            61382..63034
                     /locus_tag="PLM79_01240"
     CDS             61382..63034
                     /locus_tag="PLM79_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV84958.1"
                     /translation="MAQLSQRVIDGVYRARERFPHHAALVYLGTSFSYEALVRLIERF
                     AAALHHLGVRKDHKVMLYLPNTPQFVVAYLGAQLVGGVPVTVSPIHTPSELRYMLDDS
                     GAETIVCMDVNYRYVREAFPGSPLRRIVVTTYVDLLPRYKKVLGFLLDKVPRGRFERR
                     DDVHRFATLLGHSPPRPPRVDVDSARQLCGIVYTGGTTGFPKGCAFTHAGMVSFVDEI
                     QQVGEGHIGRGRDTMVLANPLFHQLAQGMLLGMVLNRGNTAVLMPIPETDAILDAVAR
                     HRAALLVASPTLYRRILENERLDQYSLDSLRFCWSGGEMLPAEVFNRWKKTVGRPVYQ
                     VYGSAEVGFIAMSPLDREPSPTRVGRPLPSRKTRVADPETLRPVPRGEPGELWVTSEF
                     ISKEYWNKPDETRKAYVEVDGEIWYRTNDIVFIDDNGELGYVDRRADIIQHRGFWVSC
                     SEIEAVLQAHPSVVGSCVVGVPDAKLGERIKAIVVLKEDARGVGGPELIGWCRDRLDP
                     HKVPQYVEFRDMLPRSRVGKLLRREIRDEERRKVVPKKTEPS"
     gene            63324..>64691
                     /locus_tag="PLM79_01245"
     CDS             63324..>64691
                     /locus_tag="PLM79_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV84959.1"
                     /translation="MTPEKSHDFFQVLQHHGPDRGEKTAVLCEGAEVSYALLNDHIGR
                     FGNLLKDRGVRPGERVLIALPDCPDAFYAFLGSMQCGALPVLVNPDLPRGDFEFIRED
                     SRASVLVTVGDGPAAEAWTSPLERRILVDDPGYPTLFANASPVLEVVPHSPDEAAFLF
                     YTTGRNGDLRGVPHRRGDMAFSAENYARGVLNLCQSDVVFSTSKLFLSYGMGNSLVFP
                     LYFGATAVLLPSRPITPDHVFAVIARSRPTVLFAVPTLYGMMLKTLDEGVRLDSLRLC
                     VSAGESLPAGLHRDWKEATGLEILEGIGSMETLHIFISNRPGHSRPGTSGVPVPSYRV
                     RIVGEDGLPVPSGQPGSLQVKGGSIAPFYWNRPELAAETMLEGGWLKTGDIFTEEDGF
                     YTYRGRSDDLFKSGGNWVSPVKVASILREHPRVRDCRVTSRKLEGLLKPIAHVVLNPG
                     PEGTPE"
BASE COUNT        12766 a        19560 c        19697 g        12668 t
ORIGIN      
        1 acggactcat aacctcttgc ccctttcccc tcgtctctcg tctctcgccc cgcgcccctc
       61 ccctattgca ccctggacca tgtgaagggt tcataggctt cgtcctcccc ctggagaaag
      121 cgggaaaagt gccgcacctg cctgcgaatg aggccccggt aatctgtgtg ttccccggtc
      181 cgagggcacc ggatccgggt ctccatccat ttttcgtagg cctggatgaa ggcccgggcc
      241 acccggggct tcatttccac cgggcgcttt tccttgaggt cttcttcgtc cacggcgtcc
      301 gtgcaggcca cttcccgcac cacgaaatcg tccggggcga tcaccctccg gttgatgagc
      361 cccaggacca gccggtcccc gaggaaattc ctccattcct cgaccaggtc gcaggccagg
      421 gaggggcgcc cgtattccac cgcgtggagg gcccccaggt aagggtccag gccggcgatc
      481 ttgacggcgg tgagcacttc ctgagtgagc agggtgtaga cgaaggaaag aagggcgttg
      541 acgggatcca ggggaggacg tttgctccgg ccttggaacg aaaaccccgg cgccctcacc
      601 aggaggggaa atgctttgaa atacaggttg gccgcatgcc cctcgagacc ccgtaccgcg
      661 tccaggtccg tttgcaggga aaccaggtcg gcgatcccct tgagctgggc cgccgcctgg
      721 aggagttccc ggcactcgaa gtgggacccg cgcaaggcca gcagcctgct cgaattgcgc
      781 accttgcctc gaaccaggct cccggccacg gccagggccg tgccaggatc gtgcagccgc
      841 aggtactggg cctgcctgcg ttgaacgtgc ttgtgttcgt ccaccagcag gcgggctcga
      901 aatttgcccc ggggagtcaa caggaccgtt tccacccggt accggaccag ggcgtcgagg
      961 acggcgccgc tcaaggaggc gaaccccacc aggacgagct gttccaggtt ttccagggga
     1021 atttctccca ccagggcctc gtttttcatg aggcgaagat tccgccccga ttttcgcagg
     1081 gtggatccgg gctcgaggac gtacattctt tccatgagcg ttcatctccc cggtagggcg
     1141 gtcccttttc cccatggatc ccggcggccg ggaacgcccc acatcctcct tgattccccc
     1201 aaactttcgc ggtcccgagg gctgaggccg tcctgcccga gactgcaggc atccgggagc
     1261 tatgcacgcc ttttacggat tcccccttgc gcgggaatga cgaaatgggg ttcgtacccg
     1321 ccaagtcgct caatccgggc tcccgccgag cagggcttga aggcttgcac ggcactctcc
     1381 cgagaccaca aagacccctc cgccgggttt gcttctctcc tacggatcca cgaaacgctc
     1441 gaccacggag atggcgtgtt tcagttcctc gagggcctcc cggaggttgc cgatccgttc
     1501 ggcccggttc gccgaagcct cctccttcca ctggggccaa tggagcaacg gatgggcata
     1561 ttcggggacc ctttcgaacg ggcacaggtc gcccgtaagg cccaggatgg cgtggatctt
     1621 gcggcaaccc aggggctttc cccgcagcct cgagaggcgg aaatccacca ggtgggggtt
     1681 gtactcggga agctcctgca ccaggtgatg caccagggtt tttccccgat cgagaaaacc
     1741 cagggtcccc aagaggacct tttcttcccg gacggagagg gtgcgggcct gcctgcagct
     1801 cacaaagatc tgggaaaggg ccgtgcaccg ctcccccagg agggcgagct cggggaaccg
     1861 ggcggcccac gcttcttggc gggggtggac catgacctcc gccccttttc tctctccggc
     1921 aacttccatc acccgggacg aagcgatgac cggggctttt ctgagggccg ccagctgtgc
     1981 cggggccgac cggtcgggaa cgtggaggaa aaaggacggc cttcccgtcg cccggtggat
     2041 gcccagggga agcttcacca ggttgcccag gccttttccc gccagctggt cctgcttggg
     2101 gaagacttcg agggtgaagc aggacaggtc cgccgccagg tgccggacga gttcctggag
     2161 cgcccggcgg gccgcggccg ccgggaccgg ctccgagaac aggtaccaga aatgaaaccc
     2221 cttccctccg ctgaattcca ccaggcaggg caactttttt tcccggccga tctcgggcag
     2281 gcgccccagg agatatcgct tttcccgggc cagtagctcc cggtccgggg aactgagggt
     2341 cccggcccga aagcggggcg ccaggtcggc gtccaccacg gccaggcgca cccggctgtc
     2401 ccgctggagc aaataaatcc cgtaggtctt gagaccgttc acatgctctt ggacatcggc
     2461 cgccgtcatg gggcgacgga cgggcacgta tccctgggtc ccctcctttc ggtcggccca
     2521 ctggcgagca aagcagtctt cccgcccctg gaaaaggcgc aggtatccat ccaggacttc
     2581 ctccctctcc cgcatggccg cgaagggggc ctcgaggccg tcttcccagg gaggaaccgg
     2641 agccgggtcc atgggggcgg aaagcggcgc cccggcgggc ccgggtgttc cgggggtcgc
     2701 ctcttcgttc ggaccggctg ccgtcggagt ccccgaggac ctccgcgttc cttttcccag
     2761 gatttcgagc agttcgatcc tttgcttcca tgcatcggtg ttctcgggat gccgggcatt
     2821 gacccaggcg aggacctcga ggctcaattc acacaggccc gcgacctggg ccaggcgtgc
     2881 ccaacgcacg gcttcttcgg ggggcagtct tttccagacg gtctcccggc gaaggatcct
     2941 gtccaggcga tccctcccgg cgtcggtgag agcggcctcc tcgaggcgtc gaagaagcag
     3001 cccgaagtcg ggagagctcc cgggtttctt gacgacttcc tgttgggccg gttctgcctt
     3061 catggctgga tcgcctcctg tttgcgacag gccgaatcaa gggtttgccg gggatgagcc
     3121 gccgggcgcg gcctcggcca gggccttctt taggcgcccc aggtgcggat gccacgggtg
     3181 agcctcctcg agccttcggg ccgcccccgc ggcctcctcg aggcgcccca gccgcaccag
     3241 ggccgcccct tcccagagca gtccgtcggc ataggaattc ccgtactggt cccaaaagaa
     3301 ggcctgggcg tcccgagccc accgaaggca ggattcgaag tcgccccggc gatagtcgat
     3361 gcgaaccagg cgcaggaggg ccttgtgccg gcttcgccga tcccgctccg ccgtctcgac
     3421 gaggattcgg cgcgcccggt cggggtctcc ttcggccacc aggacgtccg attcggtcca
     3481 tcggaaatag gggcgccgcc gctgggccgg aacccggtca acggcctgcc gggcccgggc
     3541 gaggtcccgg cgggccaggt gcacccgggc caggagctcg aagacataat cgtcgtcggc
     3601 cgggtccgcg aaggtggagg caaaatccag ggcgtcccgg gcctgatcga gctgccccag
     3661 gcggaaatgg atcttgccca gggcaaaatg cttgtggacc atcttgaggt gttgggacga
     3721 ggcgtcctcg tccaggcacc gcgccagggc ttccatggcg gcccggggct tttccgcatc
     3781 cagcaggcag gaagagagct ggtagaggga cttgatgaag tttttccgct cctggtgccg
     3841 cttcttgcga tcctccgccg agagggcgtc ccagtttttc acggcggctt cgaacagggg
     3901 aagggccttg tccttttggt tctggatctg cttaaaaagc atcccctgcc ggtagaggcc
     3961 ggtgactccg tcggggcgga gccgccgggc ctgttcgaaa tgagtgtggg ccagttcgat
     4021 gcgggccttg cgttcggcgg ggtggagcat cacccggcgg gtcttggccg catagaggct
     4081 gttgtaggcc gtgtaggcaa ggcccgagtg gtaatgaaaa ttctccggat ccatttccac
     4141 gcaggcgcgc cccaaggcca gggcttcgtc gaagcggccc aggtgatcca gggcgaaagc
     4201 ggcttcggca aggagtgggg cttgaagacc cgcggccacc agttccggtg ccctgtcttc
     4261 cgccgggtga aggagggtga agatgtcctc ccagcggttt tcgtcccgca ggactttgac
     4321 ctcccccagg atgcgctttc gctcgtgaag cagccgatcc tgggcgatcc ctccctctgc
     4381 caggcggtca aaatagtccc cgtgggcgga aacgctttgg gcggagccac gggaatcctg
     4441 ggagggggcc gggtttgggg gagccgtctt taccggacgg tccgccttgc cggggtgctg
     4501 gatgattctt gccacggctt tcagttccat cttgaatcct tccgtgctat agaatgggac
     4561 acgcattgaa actcacccct tgcaacaacg atttacggcc ctacggatcg ctttttcaaa
     4621 aggaacgtca tgaaaaccgt tcctcgggga ttccacgcgt tcgcgcgggc cgccccctgc
     4681 cgggcggccg gccgcgggcc gatgtttgga ccggccccga gaacccgtcg gccgctttcc
     4741 cgcggcaact gcagcggcga tcctcgatca ggaggcgggc cgctttgcct gcatgccccg
     4801 agaggttttc caatcttttt tgtaaaacgg tccatggaag ccgtaatggg aaatcgagtc
     4861 ggcgcaaccc gccgaaacct cccccggcga tgctttgggg caacgagcgg aggcggcggg
     4921 cgcacgatcc tcgaggctga gtagcgagaa aggaaggctc cgatgcaaac cgggaatcta
     4981 ttggcctccg cggaagcagg cagcgcatcc ctcccggagc aaggtcgaaa gcttagcgag
     5041 tccctggggt cctttcgatg tttacggctg caggtcgagg tgagggcccg aaacagggtg
     5101 gccctgcctc cctaccgggg gagcaccctt cggggggctt tcggactggc gcttctccgg
     5161 agcgcctgcg tgctccgaca gcagaagtgc cccacctgcc ttttgcggcg caaatgcatt
     5221 tacagctaca ccttcgagac ccctctctgg ggggaaaacg gcgaactccg gcgctatgcc
     5281 gcggcgcccc accccttcgt gctgaccctc gaggtgggac gggaggaggt tcacgaggcg
     5341 ggaagcgagt ttgagttcgc cgtcaccctg gtggggcggg ctgtcgactt tctcccgtac
     5401 tacgtgcacg ctttccagcg catgggggag acgggcatcg gcaagggtcg gggccggttc
     5461 gaggtgctcc gggtcctcga gatggacgtt ggagacgaaa tcgccgggcc ggtttatgaa
     5521 ggcggggtgc tgcgggttcc tcgaacggtg ctgggactcg aggacgccct ggccgtcgcc
     5581 ggggagcttt cgggggacgg ggtgaccctc gatctgctga cccctctgcg gctggtatac
     5641 gggggagagc tctgcaggga accggcgttt cacgtcctgg tgcgaaacct gctgaggagg
     5701 ctccacaacc tcaccctgtt tcattgcgac gacggggctc cgcccccgga cggagctctc
     5761 atcgacctgg ccgagcaggt ggtcccggat cggcgggaca tccgctggca cgactgggag
     5821 cgctattccc acaggcagga gcggcgcatg aagctggggg ggctcctggg gaagatctcc
     5881 taccgaggag acctcgcccc ctttctcccc ctcctcgtcc tgggctcctg ggtcaacctg
     5941 ggaaagaaca ccagcttcgg actggggcga taccgggtga agggagcgga tcgaaagtag
     6001 gatcacgatg ggaaattcca ggaggaatcg atgtcgtaca cgatttccga aacgatttta
     6061 ggatgtcttc tccacgatgt gggaaagctt ctccaacgcg cctacgggcg aatagaagag
     6121 acaggagcga aacgctatgg gttggaatcg accttatgtc cgaccaagaa cggggattcc
     6181 acccataaac acgttctttt tacaaacgcc ttttttgacc tggtgaggaa ggaaaatcta
     6241 ttcttccccg agggagtcaa ttgcctcacc gttgaacaca tcgccagcta tcaccacaaa
     6301 ccgggagcct gcaacatgcc cggcgccgca tggctctgta ctctcggcga ccactattcg
     6361 gcagggatgg accggcgtgc ggatgaagag tcggcgcagc gtacctcctc gatgtcgggg
     6421 taccgaacca cacctcttca gtgcattttt gatgaactca tcctcgatga ggcggccttg
     6481 ggaaagccgg aaagacatgc ctatgccctt gaagaactga atccggagaa acctgacgcc
     6541 ctgatcccaa tggcgtggcc ggacaatgga accattcgcg atcttccgaa ccgctatcgg
     6601 gaggtatggg caggtttctg ggctgctttc aaggccttgg gccgtcgaga gccgatgctt
     6661 tccttcaggc ttttcgaggg agttcttctc ggactgctcg agcgctttac ctgggcgatt
     6721 ccttccagca cattggattc tccggacatc tccctttacg atcattcacg aaccaccgca
     6781 gccatcgctg catgcctgca ccgttatcat gagaaccgcg gtgaattgga ggatgtgaag
     6841 gcaattcgga atgaatctca acctaagttt cggtttctag caggcgatct ctccggcatc
     6901 caggagactt tattctcatt gcagacgcaa ggcgttcgag gggtgaacaa gattctccga
     6961 gcccgttctt ttatgatgag cgctatatgc gaagcggcaa cactcctgat tctcgacgcc
     7021 ctggacctgc ccctgtccaa cattttccaa caagcagggg gacgtttcct tattctggtc
     7081 cccgcccagg aggatttgga aaagctagtg gagggatttc ggcagagaat cgatcagtgg
     7141 ctgctcgaaa ggtacacggg aagccttgcg ctcaaccttg ccctttctcc accattctca
     7201 gctgcaggtt ttcggccggc tgggctcagg gaggtcatgt ctcaaacagc acaggccgtc
     7261 gaggaggcca agcaacgccc cctctccact cgttctcacg gggtattgaa acgagaattt
     7321 cccttggatt catcctgttc cgcctgtggg actcggccgg ccgagattga ggatgagggt
     7381 ggacttcggt gccttacgtg cgacaatgag attcgggtgg gcaagaggct tgtgcgagca
     7441 aacatgatcg tgtggggaca ggggttgccc aagcgatggc aaaccacaaa tgttcttgga
     7501 ctggaaatgg ggctgttgga caaagcgccg gacgagagcc tggaggaggt actgaccgtg
     7561 cagaggacca acaacgatcc ggcaccgttt ccatggccgg tgaaagtcct tgcaaaccac
     7621 gtcccccttt ttcgagacga ttacgcagca catgaccccc ggtatgagtg gatcaaggat
     7681 gaagacacag ctcagggggc gggcgaaccc aagagcttca tgcacatcgc tgcggaagcc
     7741 ctggaagccg aaaccgccgg agggttcaga gggagggcat tcctggccct cttgaaagcg
     7801 gatgtggacc gcctggggtt ccttttcagc ttcggattga ggcgcaacga gatcaaggac
     7861 gatcgtttga ccctttcccg cctggcacag ctctcccgaa tgatggatct ctattttacc
     7921 ggatacctca aaggactgct ccaccgcgag tttccggaca cctacactgt gtacgcggga
     7981 ggggacgatc tcttgctgat cggaccctgg cggcagacga tcgagctgtc atcccgaatc
     8041 aacgacacat tcagaagcta caccggtcat aatcccaacc tgactctctc gacgggcctg
     8101 accctgttga agcccaatca tcccgtcaat cgagccgcga aagaggccga agggtttctc
     8161 gatatggcaa aggatgaggg gagaaaccgg atttgtgcgc ttattccgaa ggccatcacg
     8221 tgggaccggt atcgtgagcg gttacgggat gcggattgga tccatgacag gatgcatgat
     8281 gaagcccccg tgagtaccag tttcgtctac cgggtcctgg aactcgccgg agatgccgag
     8341 gctgtcacct tgcgggggga tgtgcgtaaa gcgggatggc gggcacgact cgcgtatctc
     8401 ttggcgagaa acatcaaggg gcgcggcagg gaagacactc agcatcggat catcgagtgg
     8461 ctggagcgcc ttaaactgga cgaccaattc aaattgcggg gagagcatcc gaatcttttt
     8521 gactggcgac tgccgctctc catcgcattg tatcggaacc gaagctaaag caaggagaaa
     8581 cgaattatgg caccacaaca tcaccatcag caaggacgtt cgcagcagag tggccgcgga
     8641 ggccagcagt ccgccggaac agatcagctt cccgtccccc aacccctgag atattactcg
     8701 gacgacgcaa aaaagcagct aagacccgag ttggtggacg aggaagccca cgattctgcc
     8761 aggagattct ctgaactgaa gtccacacaa atgcgtcgct tttacgatga attcaaggcg
     8821 atcgaacgaa agatcctgag cggcggaggc ttgcaggaac aggaagccaa tttcgaaagg
     8881 gatcgagccc tgatcatgat gttcaaagcc aaggccgtat atgccgaaag gcgcagggtc
     8941 tctccacgag ccttcactga attcatcttc aaccacatgg cctccattaa ggatcttcgg
     9001 gactttcagg ctttcctcaa ggtttttgag gccgttgtgg cctatcatcg tttttatgcc
     9061 aaggataatt gagaggagca gaaaatcatg aggctgctga acagcaagac tctgacgggt
     9121 cacattcgcg tgatcaccgg cctgcacatc ggggcgggca aagacgccat cgaaatcggc
     9181 ggggtcgaca gccccatcgt gaaaaacctg tatagttccg aaccctatat cccgggctcc
     9241 tctttaaagg gaaagctccg ttgtcttatg gaatgggcca ccaacagggt cagagatgat
     9301 ggaaagacgt gggacggcgg caacgaagac gacccggtat gcctcgccca ggacccggtg
     9361 ttgcgcatat tcggcacaac caataagtca tggaatgcag gccccacccg cctggtggtt
     9421 cgggactgcc ccatcaatcc gcagtggcgt gacgaaatca ttgagcgtgg actccccttc
     9481 acggaagaga agttcgaaaa caacatcgac cgcatccaag gcaaggcggg cgttggaatt
     9541 cggaagaccg aacgggtgcc cgccggcgcc ctgtttgacc taaccatggt ctatcggatt
     9601 tttgacaccg gtgaccaagg caagcatgac caggagtgct tcaaggcact gttgcaggtg
     9661 atgcgtctcc ttgaacagga tgccctggga ggatccggct cccgcgggta cgggcgcatc
     9721 gaattcgacg gcctgtgcgt ggacggcaaa cttttccagg atgagtttcg gaagattgcc
     9781 ccgcaagaga tactcgaact ccgtaggagt acttgaaatg cccgtctacc gtatcgaact
     9841 gactctgaag ggttcgttgg ggaccgacct gctctccggc acggtctggg gacacctcgc
     9901 ctgggcttta cgttatctcg agggagaggc ggccctcatg gattggctcc aagaccaagt
     9961 cagtcgtcca tggctccttt ccagccggat gcctgtgggc atgctgcccc ggccgatcct
    10021 caaacctgtt ttgaggaaaa ccgtccgacc ttcactcaaa gaaatggaac tggagaagcg
    10081 tgccaaaggg ctcgcctaca ttcccgaaac cgtctttcag ctgctgcgca acgaaatgtc
    10141 cgatgaagcc cttagcgatg tcttgaagcg tggcatggga aaatggagaa tcaaagaaag
    10201 tctcccacga aaggggatca attcccacaa cacgatcgat cgtctttcag gaacaacccc
    10261 ggatgtcgga gggctctttt tcgaggcagt gttttacccc ggtgaggaca cgaagctcca
    10321 gatctttctc cacacgccgg acccatgcct cgataggatt gaggcactat tcgccttcgt
    10381 tggagaaaac ggattcggca gcaattcgag caccggaaat ggaaacttca cctgcgaggt
    10441 gaaggaagag tccaccctgt tttcagcgaa gggcaatagg gccatgagtc tctcccacgg
    10501 cgtgatcacc gagagcatga aggacgcccg atacaaacaa catgtccatt tcggcaagct
    10561 gggcggcgac tacgctaagg gaagttacag ccccttcaaa tacccgatcc tcatggcacg
    10621 gccgggagcc accttccaac ccgagggcaa cggacccttt ggcaaactgc tggatgccgt
    10681 ccaccatgac ccggagctga aaaagatccg gcaccatgcg tttcatcttc cattgtatta
    10741 cacggaggtg cagtcatgaa ggcctaccgt tttcgagccc aagctcttac gccggtgcat
    10801 gtaggatcgg gaaatgaaat caacccgctg gagtttgtac tgcacgaaaa ccgcttgctc
    10861 cgtttcagcc ctgcggcatt gatcggatct ttgccgccga atgaattggc gcggtacctg
    10921 catttgctgg aaagagccga tttgagagcc atccactcgt ttttcaatga tgtagatgtc
    10981 gggctggcgg ggttgaccac ggtagatata tctccccatt tcatgagaga gtacgaagag
    11041 aaggcgagca accccaacaa tcagttcagg gttgaaatga tgccctgcaa tcctcattca
    11101 agtaagcgct atctgcccgg ttccggcatt aagggcgcca tcaggaccgc cttgatcaat
    11161 cattttgcgg acaccgatcc ccataccagg gagacggtgc gtgatgcggt cactcgcgag
    11221 agagacaaac gaaaccgggc aagggtcctg gaggagaaag cgttcgatag gaaacacagc
    11281 cagacggaaa gggatgttct gcgattggtg cacgtggagg acgcgtgtct tccggatggg
    11341 gccacgcgca tcgaccgagc cgtgaactgg aatcctaaca ggaaaggggc ggagaatatc
    11401 cagatgtggg tcgagaggct caagtccgtt gcagacggcg gcacagcccc gcaattcagc
    11461 gttatcctcc atatggacaa caccaagatg cagcaccctc cggtgaaacg ctctcttgga
    11521 aggaccatcg atttcgacac gatcctcaag gcctgcaacc atttctattg gggtcgcatg
    11581 gttgcggagg gagagaaatt tgacggcaag aattcgggcg gtaaggtttg gagtgccctc
    11641 cttgatcttt ttcccaaagg aaagactccc gacgggcaga ttgtttccat cgaccctgcc
    11701 aaaccttatt ggtgctatga gcaaaggaaa agaatcctgc ttcgcgtggg ccgttattct
    11761 cacttcgagt cgttgtccgt ggacggactt cgggaagggt acaacgccca ggcgagacgc
    11821 cccatccggg acatgggctc caccaggacc cggtgcctca tggacaatgg aaaacctccg
    11881 atgccttttg ggtggatcat gatgacgctg gagagctccg attgaggtgc gggcgttgcc
    11941 cggggatgtc ggcctttgtc aaaggacggt cgtatatctt cagcccccgg atggtttctg
    12001 agccccggga ccgccgctga gagcgcttac ggggacggcg tgcatatctt tgccgaaagg
    12061 gacgatttcc cttccggtat ggatgggaat cccgcggacg aagcggtccc ctagatcttc
    12121 ccgcaggatg ttcggcccgg agaaatcgca gcgtgacgga gtggccgaaa gttcgacttc
    12181 aacggccaca gctcttccgc cgcccccttg gaggacgatg tccaccttct gtcccgccgg
    12241 ggaacggaaa tggtagagtt ttacggattc ccgggaccag aaggcctgtt agaaaagctc
    12301 caaaaccacg aagttttcaa acactctccc cgcaagctct ccgcccctca ggaatcccgc
    12361 caggtccatg cccatgaggt gacaggcaag tcccgtatcc accatgaaca tgggggtctt
    12421 caccaggcgt ttgcccgggc tggatgacca ggcgggaagc aaccgcacca ggaacgtgtc
    12481 ctgcagcacg gccatgtgcc gagtgagagt ggcgttggga atgccgctgg ccctcgataa
    12541 ttccgattga ttctgcaggg aggcaatgcg acccgccagg agggccagaa gccggggcag
    12601 cgctgcaaga ttcagaatct tcgaaagatc tcggatgtcc cgacccagga tcgtggtgag
    12661 gtaggactcg aaccatgctc ccctgcgccg gggagctttt ctctgtaggg tttcctgaaa
    12721 accgcccgcc aggacgttcg cgcccagatc aaaatccgga tcgggtcggc aacgttgggg
    12781 gaattcctct tcaaagaacc catccactac gttctccgtg gtgcccttga tttctcccat
    12841 ggacaggggc cacaaggtga gcacttccat ccttccggcc ggggatcccg ccagccgggg
    12901 cacggtcaat acgtcggcgg accccgtgag gatatagcgc cccgggtggc ggtggcggtc
    12961 cacatctttc ttgatggcca aaaaggaacc ctctcacccg ttggacatca tcgatcacca
    13021 cgggctttcc catgcctgca atgaacccgg cgggatcggc actggcggcg gagagaacgg
    13081 cggcattgtc cagggttacg tagcgggccg ggtagggtcc cttgaccatt tcttggacca
    13141 gggtgctttt tcccgtctgc cgggctcccc gtacaaagac cgccagcgta tcggccagtg
    13201 cctccaggat gtttttcaag agagatcgtc tatacatggt aagatattta ccgtttcatg
    13261 gtaaatctgt cgcaggaaaa agcccgttgc cgccctcgga aaacgggaag tgtccgaccc
    13321 ggatcccgct caggcacgat gagacgggtc gccctcgaaa agtttctcca ccgcatccag
    13381 gctgcttctt agcttctccc cgaagcgccg cccatcccgt gctctgatgc aatttcgaca
    13441 aaacccggca tgattgaggt catttcgagc ggacctaacc ttttccactt cctggcacag
    13501 atttccctgt gcctggatga acccgatcat gcgccgagtg gtatccgggt gatccttggc
    13561 gggcgagtac cattgttccg gggtatcatc ccatttacgg ttgacgaggg tgaaggcctg
    13621 agaggcaaca tcccgcaacg tggtatccag cggatcggcc cctaccccca ggaccacgta
    13681 gctgaaaagc agttcctcca ggaaagtgaa tccttgttgg atcaaaccgt ggtcgaggca
    13741 ccaacgaacc gccgtaagac cctgtcggag atcgtcgccg gcaaattttt ccagtcgttt
    13801 ctcgatggtg tcgaaaagag gcctgaacgg cggcagcagg tcaagacctc tgcactgttg
    13861 tacctgctcc ttgagagcgg ccgcaaccgc cgtgatttcc tggccgcgac aggtggagag
    13921 catgtcgcta aaccggtgaa gttccgttcc cagcttcctt atggtatcag cctcccgatc
    13981 cttcccccgg gtttctttca atatggaccg aacgctcgct gccgccaggg cccccgccat
    14041 gcgggcatcc ccgctctcga ggaagcggtg ggtggcaacg gtccagtcca tgagttgatc
    14101 caaggcggtg aggtccaata tccgcacttt gcgggactcc acctccatgg ccttgacctg
    14161 gtcatagctc cccaatgctt cgaaagcccc ataatagata ccccggagac tcacgtgctt
    14221 cagagctttg gcgtagttca ggaccacaac cgccaccagg ggaatggaac gaagggcatg
    14281 agtgatgtcg aagaccacgt tgtcgccggg gcacagacag tcattcaacc gtttgaaaat
    14341 ctgccagatc tcgtcttcgg aatacccttc ggggattgtg acgttttcgt ggggcacaga
    14401 aagttccatc ttcccaatac agcatctgag accctttctc tcgagtattt tgccttcttt
    14461 gttacgatgc ccgttgtcca gccagttttt gcgttccgct tcctcggtgg tgaagatgag
    14521 aatccggtcg tccgcccccc aggtgctgca gttgtttttt atggtggctt gttgcacgaa
    14581 acggacgggc cccaccggtt ccaagccatc cccgcaatag atgcattcca ggtaatcatt
    14641 ggttccaaga aaggagacgt aaatgttggc catctgctat cctccatacc ttcgaaatcg
    14701 tttgtgccgg aaatgatgaa tgagccggat ggagccgtcg ggttggggct cctcgaccgc
    14761 ccgccgttcc gtggtggaat agacgggatc gaggccgatt tcccgggcaa accgcaccat
    14821 gaggcaggtg gctccgaagt ccccctggat gagaacggcg tcccccggct tggccgcctc
    14881 tttcacccat tcttccacgg gggccaggta cccggccagg gtttccgcct ccgggggaat
    14941 gtttcgccag agttcccgga cgctctcggg aggatggagg aaattcgaaa cccccagggt
    15001 ctcccgggct tcggcttcct gggcgggagt caacgagtgg ttgaaaagca gaacgaggtt
    15061 ttttgccatg agtcaccgtc ctccttggga cgctaaaaaa ttggaaaggc ctccggggag
    15121 ttgcccgaag attacggctc cccgggctcc cttttcaacc ccaaaggcga ccctggcatc
    15181 cgctttttcc ttatggtaag aaaaggaaca tgccgacggg cggagctcgg ggcaacaccc
    15241 gccgcggcag cctcgcagtc gcatcaccgg ttcactcgta tccaaacagg aaagagcgga
    15301 ggtttcgtgt ccacattttc ctcccgcacc gccgccattt gggagtcgcc ttacggaccg
    15361 gtcccacccg acgcggacct ggcggtcctg gggtcgggaa tcaagtacct tcctcctcta
    15421 ctctggaaat cccgcatgct cttccggggt gccctcgacc ggatcgtaaa gaagctcacg
    15481 ggggatttcc cccacaccct gggacgggga aagaccactc atcctctctt tgtactcaag
    15541 tcccttccga acccggccca ccgggtctgc ccgtgttctt ccaaggattg gggagctcgc
    15601 cgggttatcc ggaaggggtg ccgtctcgag tacaccgggc aaatcacgga gcgcgacacg
    15661 tttcttgtgg agtcctgcag cttcaacttg cccatggact cggccttcac caaccggctt
    15721 ttcttcctgg gacgggtccc cgaggagtgc atcgagacga gggattcatg accgattacc
    15781 aaggctggcg cgattggctt gccggccccg gttgttggcc ggccatgacc gaggcggccc
    15841 ggttcgtgta ccggatggcg acgcgggtgg ggcttccctc gagcctcctg ccctgtggga
    15901 acccgtggag cctgccccgg gaggaaaggg acgagtgctt cgaagaactg gcccgcgaga
    15961 tgtggctgtt tctgcgatcc atgtcggccc aggagatgaa gagaatcggc gcggacccga
    16021 cgacccatgg gaacacccgg ctgctgatgc agcgcatcgg ccaacggttc ttcctccatc
    16081 tcaaggacca ggcccggaca tccccgcgag accccgagcg cgccctgtac cggcggcttc
    16141 gccaggtgct ctcccaggag ccgaccgtcg cctaccggac cactgccgac ggggcgtttt
    16201 actcccggga ccccggggcc ccctggcccg ggacccggaa atccctccac gaggagccct
    16261 actcgagctg ggagtcgccc ctggggagga tcggtctaag agatttgccc cgcaggagcg
    16321 cccttctcga tgtggcggat tttttctggc tccaggcaac cacccggctg ggggagccct
    16381 cgttcttgcc cgtccgggag ctggcccggt tcatcgcgtg tcactacccg ggtttttcct
    16441 taccgacccc cgtgtccatt cacccgggag gaacaaaggc ggattgcctc gagggtcggg
    16501 aggtcgaggt tccttcgggg gagccgggac cggatatcca gttcatggcg caacggcttc
    16561 ccgccctggc ccggcagctg gttcacggct gggacctgag acaacgttcg gccttcgttc
    16621 tcctcgtggg ggacggcgtt cccttgcagg aagccgcccg gagcctcggc taccggaacc
    16681 cttcgggggt ttcctacctg gcccggtcgg tttacgacag cctgcgggac ttctgtcttc
    16741 tgtggcccgg gctttcccct cccgacctcg atcccggcct cttcgaagcc ttcgtgctgg
    16801 aagtcgtcac cgtttgcaag aaagggacct aaagccgtat ggttgtattg agtagaccga
    16861 tgccctcggg tgtccaggac gggagtgtat tccatgtgtt cctatgaacc ctggcagatg
    16921 aaaatggcct ggcagatcgc cctgcaggcc cggggatgcc ctcccgactc ggtccttcgg
    16981 gaagggaagg cggacccacg gctgcaggcc catcgcgacc ggtgcccctt ctgttccgaa
    17041 catgggacgg tggaagcggg agacaggacc gtggaaacgg ggcttttcga agggaccgcc
    17101 cttgcccggg agatggctcg ccgggtggag atcgaccacc acggagaacc tttgccggga
    17161 tcgatctgcc ggatttccgg ggccctggcg gggtggggtc cccgggagcg gtattacaac
    17221 cctcccctgg tgctggtgct cgatcaccct cgggacatgc cgggagccgc tcgggtggcc
    17281 caggtgtacg acgacccctg cctggcgggc cccggggacg ttcccctgga tcacgggctc
    17341 tttgccgaat cctggaacac ttacgccctg cgggcggaag acctggaatg cgctttcggg
    17401 ttcgtgaacg aaacgaagct gagggaagtc ctggacgccg aggcctccga cgggccgggt
    17461 cccaaacaag ggacgatcct cgaagccttc cgcagactcg aggtggaagt ggccgccttc
    17521 tttgccctcc aggccctggg cgacctcatg gatcgccggg aattcggtcc cctcgaggaa
    17581 attgcggccc gctttcccca cggggaatcc ctgaagaaag ccctggccga gggggagccg
    17641 catatccggt ggcccaagga caccacggac ccgagggcca tgttgaccca cgcgcaggtg
    17701 gccgaggatc ggcttcccat ggcggcggcg ggacaagaag aatggatcac ggccaacatg
    17761 gtcgtcctgg acggcgagac ccttcgcctg gaagccgtct tctgtgaaat ccgccattac
    17821 tcgatgcagg gaaacgcgct ggtggtgggc ggcaaggtcc ccccgtcccc ggccgggagg
    17881 gccgagatca ccgcttcatg ggtgaaggag ggtctgccgc ccagggattg cgatcaatcc
    17941 ttcctcgatg aagaatcggg cttcttccgg ttgctctttc gggacatgac cgaagccgaa
    18001 ttcaagtccg ggcggcttct ggtgctggtg gcgggacttg caaaatgagg agatggtcct
    18061 tcgaggagtt gggggtgtgg gctcgtcgtc actgtctgca cgagcggatc tccgacctgc
    18121 tgtatccccc tggattcgaa ttccccgcaa cggaggaaga cctggttgga tcctggaagg
    18181 cggtcgagga cgccctcggt ccccaaaggg tccgggtcct ggcccgggga tcgtgggcag
    18241 gggccctccg ccaacgaaga tccgcccctc cctggttttt cccagccctc gaccggcttt
    18301 ccccctggaa ggacctcgat tttccttttt cctgggccga ggccgccgag gggcgctgga
    18361 gcgcctttgc cctggccctg gcgagggcgg gcgagggcgg ggtcctgtgg gccgtgatgg
    18421 gacaactcga gggggcgatc cggggcaaac gatggccgaa atggtgggaa gaggcggcgg
    18481 acgttcatgc ccgggagggg gcggcctttg ccctggacat gctcgaaaga gagctatcga
    18541 ggtccttctt cttttggccg atcctccctg tcccgaaccc cccggcggtc tggggaaggt
    18601 ctctcaccct ccccgtctac ctggccggat gggccctggc ccgtaatgtg gagaatcgct
    18661 cccttgtttg caccggcaac gtgctcgaag acggttccct gggggaggcg gggcacctgg
    18721 agcggaaggc tcgactggcg gcaattcacg gtttcaaggg gctgttgtgt ccccggggga
    18781 gcggcggtct ttccttggga gccgagggca tcgagatcct cgaggccgac gacctcgatc
    18841 atgccggctt cctttgggag ctatcggccg ggggaacgac cggcggcctc caggaagatt
    18901 tccgctcctt gagggaccca aaccgcctgg ccgccaacgt ccaccttctt tccccggggg
    18961 ttttgcgctg gagcggcttt gacggtccgt atgccgccgc actgcgggaa atctcccgat
    19021 cctcgaccct gatcgaggac ttcgtccgaa acctcgaaag acgcgtcgaa gatccgaaca
    19081 cctcccagga ttccctcgat cggctcctgg tccccgtcac ccgggaattt ttgatcgacc
    19141 tccgggaaat ccatcccctg gctgccctga aactggccca ggttcgaatg gcgacggcca
    19201 gtcaccgggg aaggatccgg gaagcggaag agtggtcgag caccgctacc gagctcaccc
    19261 aggcggccct ccgacacgcg gagggactcc attgcaaggc cgacctgctg aatcgcgaat
    19321 tcgttcatca tcgccacaat cactacgact tccgcatgga tctgcccgaa gaggtccgga
    19381 agaccctgga caccctccgg gccctccacg ccctgctcaa gcaagaagac cccgactgta
    19441 ccctgccttc cttggggaag ctctacggta ccctggctca aaactgcggc ttctgcggtc
    19501 ccgagcacct cgaggaactg tgccgttatg cggagttggc ccgggaagcc tttggaaacg
    19561 gcagggaccc cctctacctg gcggactggc gcagacagtt ctgctacgtg ttttacggcc
    19621 ttctggatgc gggagacccg gcgggggcgg aggaaatcct ttccctgtac ctgggaaccg
    19681 agccccggag cctgaccgac gggcggattc gggttctcac cccctatgag cacgccgcta
    19741 tggcccgctt cctcgccgag accggcgcag atgttcccga atatcgacgt tggatgagca
    19801 tccacctggg ccgtcgtccc tccaggcatc cctggcccct ctggctcatg aacgccgggc
    19861 tttgccggga agaccccgcc catcgccggg aggcctttac cgagtccgct ctggcctgcc
    19921 tgagacaggg gcccacggcc caggcgatgg ggctcatgcc cctccagctc ctgtggaagg
    19981 aaggtcttca gcctcatgat tgggtacggg acgggacacg gcagattctc caggaaatcc
    20041 ggcattcttt cggcgggcaa acccactttt cccggctctt ggagtattcc gattgggaag
    20101 aagccctctt cgaggtcgag ctgcagaagc ctcgtctctt ccccttcact tatcggtgag
    20161 gggttttgcg gccaactccc cggccatctc ctcgaggagg ccgatatggc ggtgctcctc
    20221 ggcgatgacg gcccggatga gctcggccgt ctccgcggac ggcgaaaagg acaggaggat
    20281 ttcgtagaag gtgatcgagt ccttctcgaa gcccacggca atttgaagca gggttccttc
    20341 gtcccgcacc gatgcaaaat ccgcctcttc gagggaaaaa gcccggtctt cgatgaagct
    20401 ctggaggacg gcccccgaga tgcggtccgc ccaaaggtcc tcgtcttgcc ggcctagctc
    20461 ccttttcatc cgataaaatg tttccctgtg ccgcacttcc tcgtcggcca gcttttcgag
    20521 aagcgatcgg acggcggaat cgtccacctt ttccattccg tcccggtaga accgctcccc
    20581 gttttcctcg atctggacgg ccagatcgaa cacttcgtgg gccgtaaaca tccgttcccc
    20641 tcctttgctg cccggcacaa cctcgttccc cgggcggcaa tcctccccgg aatcgcaacc
    20701 cggaacgggg tcgacgtcca tcgggttccg cccgactccc gggacttccg tttcccggac
    20761 tctccacaac ctgcttgcag cgggaaccat ttcccgctat ctgaatgggg tcgaaacccg
    20821 gcttggatgg tagcgacccc ggccgggccg aggttactgt accaatgctt ctttttccat
    20881 gccaggagat ttcgctgccc atgaaaacgg gaaatatcac cgccttggtt ccccgggatc
    20941 gagaccggga agtcttcgag gagctcgtcc gcaccgatcg ggtcaccatc gaacgaatca
    21001 tttcccacgg tcaggccagc cccgagggct tctggtacaa tcaggagcgg gccgaatggg
    21061 tcctggtgct caagggaagc gcgggcctgc gcttcgaggg ggaggacgcg gtgatggttc
    21121 tccgggaagg ggactggatt cacattcccg cccgtcggaa gcaccgggtc gagtggacct
    21181 ctaaaacgga agccaccctg tggcttgcgg tccatcatgc ctgatccgga cccggtttga
    21241 gggcttctcc aagaagggtt tgttcccctg cccccgaccg ctctcataga gagaggaacc
    21301 tggatcgagc gatttgacgg ggacgaatcc ccgtttcgtc gttcccgcgc aggcgggaat
    21361 ccggaaagag cgtgcgtacc caccggacgc ccgccttcgc gggcatgacg cccggatgct
    21421 ctcggaactg ctgacctcgg gaggaaagga ccggaatccc ttgtcgaggg aagggaaaac
    21481 actcccactc cccgaccggg gaagattcct gctcgatatc gagcccctga tttcatgcgc
    21541 cccttccccg ggggccgcct tgcgggaggt gactatggac agaaaggatc gggccgtcga
    21601 gttgctgacg gagcttagcg gcgcccacgg ggcgcccggg tgcgaaaacg aggttcgcag
    21661 gatcgttcga aggtattttc ccacggccca tacggacccc atgggaaaca tcttcgtgga
    21721 aaaggagggg tcggcatcga ccccccggat cctcctcacc gcccacatgg acgaggtagg
    21781 ccttatggtg caggccgtcc tccggtcggg gctcatcaag ttcgttcccc tgggcggctg
    21841 gtgggctcac accctgcttg cccagcgcat gcggatccga accggggacg gccgggagct
    21901 cccgggggtg atcggggcca aacccccgca cttccttagc gagagcgaac gggacaaggt
    21961 ggtgaagatc gaggacatgt tcctggacgt gggcgcccgg gacgacgaag aggtccgccg
    22021 gcttggaatc cgcatcgggg acccgatcgt tcccgacacg tccctcaccc tcatgggaaa
    22081 cccggatttt cttctctcca aggccttcga caaccgctgc ggcatggccc tcatgatcca
    22141 ggtcctcgag gaactccggc aggtccccca tcccaacacc ctatgggccg cgggaacggt
    22201 ccaggaggag gtgggagtcc gtggtgcccg gacagcggtg tacgggataa atcccgatgc
    22261 ggccattgtc ctcgagggat gccccgccga cgacctgccg gggactcccg aggacgaaag
    22321 acagggagcc ctgggaaagg gggtccaaat tcggcgcatg gacccttccg ccatgatgaa
    22381 ccgggccttc gtggactacg ccctcgagac ggcccgaagc cgggacatcc cgcaccagga
    22441 ggccgtgcgc cggtccgggg gaacggacgc ccgggccgtc catcttcacg gccggggagt
    22501 ccccaccctg gtgctggggg tcccggcccg ctacattcac acccaacaca gcatagtttg
    22561 cgtccaggac tacctgagcg ccctccaact ggccctggag ctcatcgggc ggctggacga
    22621 agaccgggtg tgccgtttca cccaatttga cgactgatcc ccggggtaga cggtcccgga
    22681 gtttttttct tgcccaacgg cccaaagttt ttcatagtgc ggggcatctt cgttggggag
    22741 atcgtgcaaa cgaaacgaag aaggacgatc cacgggggag agcggacccc gtgaattcag
    22801 caactacgaa gagggaggat ggatagcgca tgcgtaacgg gctgaagtgt ttgttatgga
    22861 ttttttccct gtgtttggtg gtgagctgtg ctcacaagga ggactcatcc aggaaggttt
    22921 cccagcaacc gaccccggcc gccccggctg cggcccccgc cgccaagacg gcgacaccgg
    22981 ccgccgttcc ctccccgggt tcggccccgg ccatccagct cgcggaaacg aaccacaact
    23041 tcgggaacat gtccgaagac cggcagtacg agcacgagtt caaggtcaag aacacgggcg
    23101 gcggagtgct cgagatcaag aaagtgttgc ccgggtgagg ctccagtgtc ggccggttcg
    23161 accggacgat ccctcccggt ggtgagggaa ccattgcact gaggctcaat cccaagactt
    23221 gcgtgggcag caccaagaag gccgtcctgg tcatcaccaa cgatcccatg aagccgcatt
    23281 ttaccctgac cctcgagggc aagggcggct cctgaggcgt cgccgccccg tcgaggcggc
    23341 caaaccgccc tgcttctgcc cgcatcatca aagccccctt ccccgacccg ggcgggaggg
    23401 ggctttttca tttgagccgt ttcgagaata tccgctcgtc gtgcacgcca aggcgggcat
    23461 ccggagggca ggcacgctcc tgccggattt ccgccgccgc gggattatgg aaacgggatt
    23521 cgtgcccgcc aaatcgctcc gtctgaaggc ttttgcgctg gtgcacccag ccgttgggaa
    23581 gcggcatcgg ggagccccac ccgggcatgg ggcttccgcc ttcgcccgcc ggaggacgcc
    23641 gacaagaacc cttgcggtca caggtgccgg taggtcagct cgaccaaggg cgacaccccg
    23701 tcacaagagc ccccgccgtc atgggtgccg gggttgatcc gagggaactc ataccaagtt
    23761 gcgcccaggg tcgtcctgac atgcagagtg aagatatgga gcaaagggct gaatcctacg
    23821 ggtggacagt ttcggcttga gtgcaacttc gtatcggggg gtatgacgac gggcgggggg
    23881 aaaccgcgta cgggaatcgc cgggcacccg agaccggaat cccgacgcag gtcgggcggg
    23941 aatcgggtcc cggcaaacgg attcgaagac acggagcggt ctgccgagtt cgaggccgcc
    24001 ggggccgccc tccttgcgca gatcgtcccc ggggaaaatc ctttcgtttc gtctaaagtc
    24061 tttctctcat ccttccgata tgttaggcat gagagaagga gcgaaccatg gacatcatcc
    24121 tgggcaccaa cccggtcaag ggatctcacc cgcgcaccac ggcgtcgggg ggcggcacga
    24181 cggttgaagc gagaatcctc taccagagac cctgtcggaa atccaggggg acgcctccct
    24241 cggggcagcg ggagcggcgc catcgcagaa gccttgcgga cccgcccaac ggccgggtgg
    24301 tttttctact tgttcccgaa ggaaaccggc tgccccggga cctcagcaac gcgaattacc
    24361 gggtctttct tcgcctggtg ccgggatcga acccctagtc ccgccctttt cccacatctg
    24421 cgattaggcg gggatggatc gccgcctccc atcgaacccg gcgggctcgc aaccaccagc
    24481 cggggttgga ggccccaagg ccgatacccc cggaacaggt cccgagagga agtcgaccct
    24541 tggggtcaca ggaccgtccc atggacagga gtgggattgc gccccacaca tccggaaggg
    24601 cggccggtcg cccctacacc ctctgcacgc gcgggacacg ccgtacggag acccgacccc
    24661 tgaggtcggc catacggaga cctgacccct gggggagggc tttgcccccc tacggctgca
    24721 ccagcccggc tccgctcaaa accatcacca gcctcctgaa gacgtccata tcgatttctt
    24781 cctgcatttc ctccttcatt ttcgtcagcc cctggaaggg agtcagggga tggtcccggg
    24841 gtcccgggga cgtggccagg tcgtagcggt tggccacgtt caccaccttt acgggcaaag
    24901 ggatgttcgc cccggtcaat cccgttggat agcccctccc gtcgcactgt tcgtgatgaa
    24961 acaggatgca gtggatggtg gattgatcga ggggaaccgc ggaacacagg gccaggccct
    25021 tgaccgggtg ggtcttgagg atttcccatt cgtgagcatt gagccttccg ggcttgttga
    25081 ggatctttgc ggggatttgg gactttccaa tgtcgtgaag caaggcgccc agcccgatgt
    25141 ggaccttctc ctcttcgggc aggctgtaag tttccagcat ggcgagggta tacaggaaaa
    25201 cattggtgga atgagtgtag acttttcggt cgtgggacat gagggacccc agggtcttga
    25261 acacgttctc cctgcacaga aagctcacgc tcgattcgac caacttcacg agctttcggt
    25321 gccgatccga agtcaggggc aggctcagct tggcctcgag cgcccgttcc aggacgttca
    25381 cggcgcagga gtagaaaatc cgggaccgca cgggcagggg aatgttgtcg tccaggagga
    25441 tccgggaaag gttcctttcc acatagtcgt caaactgggg cttatcgtcg gtgcggatgt
    25501 acacctcttc caccccgttt tcgtacagga tggctttgtg ccgttcggtg accgcctccc
    25561 cgtccttggc gtaaaggatg tggtgatttc cgaatttcag aaagatggag aagttgccgt
    25621 aggcgctggg aaaaatcatg tagggggaga ccccgaagaa cccccccggg ctcgtttcct
    25681 ttggttccct gtccatccca cacccctcag ggttcactac agcttgacgt tcgaacctaa
    25741 ccggcaatcc cgatggccag gatccccagg aagatgagga cggcacccgc ggttcgaacg
    25801 ggcccgaact gctctcccaa ctggaaccat cccaccaggg cggccagcac gatgctgatt
    25861 tcccgaatgg cgcccacata gctcacccgg gccgagagac aggcctggag aacgagcata
    25921 taggcgccga agacgaggac ccccaccacc accacgcgca gaaagtgggc ccggagcacc
    25981 gcaagggcgg gcttcgcccc gtagtgtacc acaatcaccg gggtaatcag gaggctcatg
    26041 attcccagga ccaggaccgt gtaggccccc gggaacaggt accgcacggc cgcggcgtcg
    26101 atgacggaaa aagccgagat gcacaaggcc gccgccaggg ccgaaaccag gtcctgggac
    26161 ctcgaggtcc ctttcctccg agaccaccat cctgcgcctc ccaccaccaa gagccccgcg
    26221 atgaggagga ccaggccccc caagcctgcc ggactgggcc gttccttgag aaacaggttg
    26281 gcccagaggg ccagaagggc gggcgcggcc ccccgggcaa tgggatagac cagggaaaaa
    26341 tccccccgct cgtaggcccg gaggagggcc aggaaataga gggtttcgag gacggcgctg
    26401 gtgagggcaa aggcccagat cttgggggga aacgggatat gccacaggag cacgggcagg
    26461 tagaggacgg acccggccac cagggcccac caggtgaaga ggtgtttctc ttccactcgt
    26521 ttgatcatga ggttccagac cgtgtggagg agagcggaga gcaacaccag caggagggaa
    26581 tcgaaggaca tggcgaaggt cctcggaaag ttgaatgaaa atcatctctt ggatcgcttc
    26641 acaaaccggc tctcgaaatg ttcctatccc cctaaaaccc aacgggaatt tttacactaa
    26701 gtccgcggtt tttccatgat tttttttcgt ttggtcaaac tgattttcgt tgactcaaac
    26761 cacagaaacg tatactctcg gttgcgagtt agaatgttgc ccatcctccc aggatgccat
    26821 atttgcatcc tgcagcaagg aatgcaagac gtgcctgacc gggtttcagc ttgaggtgga
    26881 ggttcatcag gatatcggtt cgacgggaat gagacggttt ttgccttttc ctgaacggga
    26941 tgtgaggggt tcggtttgga cggcaagagg cgtcaggcat gcattcgagg ctgatcgcag
    27001 ggatatttgt tccgttgata cctccggacc tttgacctcc tcggttcgca gcatccatcc
    27061 agttcaggaa acacccgacc tattgactca aaggacggcc ttcgggcagg gttcgtgccc
    27121 gcggggaccc ggcctttgtg aagcatattg tggggagcct atttcacatc cacgctttca
    27181 cgaggacacc tggacgcgcg tttttttgtg gaatgtctgc tagtgaccca acttttaacc
    27241 agccatcgag gaaagtagag ggagaatgat gtccaaccaa gcaattgccg tgaaggaacc
    27301 cgtacgaaca gaaggctatt tggattcttt ccaaatgcta tcgggagttg gtctcatcat
    27361 atttatgtgg agccacatgc tcctggtagc cagcgtcaac ctcggggcgg gggtaatgaa
    27421 cgccattgcg gatttcctgg agaaatatta cctggcccaa accggcggtc cggcgatttt
    27481 cgcccttttt ctggtccatt tcgtgctcgc ggcccggaag cttcctttcc gcatgaagga
    27541 gcagaaggag atctgggagc acagcaagcg cttcaatcac acggatacct ggctgtggat
    27601 ggtccaggcc ggaacggcca tgatcatcct catcatgggc tccatccaca tgtggaccgt
    27661 tctcacggac ctgcccatca tggcggccaa gagcgcccac cggatccagg ggggatggtg
    27721 gcttctgttc tatttgctgt tgttgcccat gatcgagctg cacacctcca tcggtttcta
    27781 ccgcatcggg gtcaagtggg gattcatcac ccgcgcggat cggaagttct ggaaacgcat
    27841 cgagaaccgg gtcaccttaa ccatggtcgg cattggtctg gtcaccctga tcaacttcta
    27901 tttcatccaa aaagcaatgt gaggagtata aatggatacg ttttacaccg atcttttgtg
    27961 tgtcggggca ggcttggcag gcgagcgggc ggcgattgcc gcggcttccc agggctatga
    28021 gaccatctgc ctgagcctgg tcccgccgag acgctcccat tcgtccgctg cccagggcgg
    28081 catgcaggca tctttgggca attgtattat gggagccggg gacaatccgg acgtccattt
    28141 cactgacacc gtgaagggtt ccgattgggg ctgcgaccag gaagtggccc gcctttttgc
    28201 cgagacggct cccatcgccg tacgggaagc cgccttctgg ggcgtgccct ggaaccgggt
    28261 ggtggcgggc aagggcacct acttcaaggg tggcaagcag tatgagaaag tcgagctgaa
    28321 ggaaaaggaa cacctgatta cggcccgaga ctttggcggc accgccaagt ggcggacctg
    28381 ctacacctcg gacggtaccg gccacaccct tcttttcgcc atggacaacg aagtagtgaa
    28441 gctgggcgtg acggttcacg accgggtgga agccattgcc atcatccacg acggggaaac
    28501 ctgcatgggc gtggtggccc gctgcctgaa aaccgggaag cttcgagtgt acctggccaa
    28561 ggccaccatt attgccacgg gtggatacgg gcgcatctac caggagacga ccaatgccgt
    28621 gatcaacgac gggagcgggg ccgccatcgc cttgaatacg ggcgtcgtcc ccatcggcaa
    28681 tcccgaagcc gtccagtttc accccacggg tatcgttccc accaacatcc tggtgaccga
    28741 aggctgccga ggagacggag ggactctcaa ggacgtcaac ggtgaacggt tcatggacaa
    28801 gtacgaaccc gccaagcagg aactggcgtc ccgggacgtg gtctcccgct ggatgatgca
    28861 ccacattcgt ctgggcaagg gcgtacccag cccgtacgga ccgcatctgt ggctcgatat
    28921 ccgccacctg ggtgaaaagc acatcaagac caagcttcgc gacgtcgagg agatctgcaa
    28981 cgaattcttg ggggtggatc ccatcaccca gatgatcccg gttcggcccg ctcaacacta
    29041 cagcatgggc ggcgtgcgca ccaacaagga cggggccgct tacggcctca aggggctgtt
    29101 cgcagcaggg gaagctgcgt gctgggatat gcacggcttc aaccgcctgg gcgggaactc
    29161 tctggcggag accatcgtgg ccggaaagat cgtcggggag aagatcgccg agttcctcca
    29221 gggctacgag gtcaacttcc gcaccgatgt ggttcaagac tgcgtgaaga aggaacagga
    29281 gcgcatcgac aacatcatca aggggaaaga cgggaaggag aacgcttacg cccttcgcaa
    29341 cgagatgcag gccatcatgt tcgagaaggt cgggatcttc cgcaacggta gcgacctaaa
    29401 agcggcggtg gaagggttgc agaaggtgta cgaccgcgca aatcacgtcg gacttcggtc
    29461 cggcggcgtg ggtgccaacc ccgaactggc gttcgccctc aagctcaagg ggatggtccg
    29521 gttggccctg tgtgtggcct acggcgccta caagcgggaa gaaagccgcg gcgcccacgc
    29581 ccgcgaggac ttccccgagc gcaacgaccg cgactggctg aagaggaccc tggccacgtg
    29641 gaaggaaggg gacgacctgc ccaccctgaa ctacgaaatt ccttccaagg tctgggagtt
    29701 gcctcctgga gaccgaggtt acggatcggc ccccatcatt ccttccgagg accctgcggt
    29761 catcggcgga aagaaaccgg aaaacaagta gaaaagagag aggtgtttta atgggaagac
    29821 cattgaaatt caacattttc agatataatc cgatggatcc ggggtccgtt cctcacatgg
    29881 aggtctttca tctggaggaa accgatgcca tgaccctctt cgtggccctc aaccggctcc
    29941 gcgaagagca ggatcccacc ctgatgttcg acttcgtctg tcgtgccggc atctgcgggt
    30001 cctgtgccat gatgatcaac ggaaaacccg acctggcctg ccacaccaag acccgggacc
    30061 ttccccagga aatcaccctg atgcccatgc ccgtattcaa gctcatcgga gacctttccg
    30121 tggatacggg gagctggttc cggggcatga acgagaaggt ccagtcgtgg attcacaccc
    30181 aaaaggtgtt tgatcccaag gcacaagaag agcccatgac caacgagcgg gcggaggaaa
    30241 tctacgagct cgaacgttgc atcgagtgcg gctgctgcgt ggcggcctgc ggcaaggcca
    30301 acatgaggcc cgaattcatg ggcgccaccg ccttcaaccg cgtggcccgg ttcatgctgg
    30361 atccgcgaga cgagagatcc gacgccgagt actacgaggt ggtgggaacc gacgacggca
    30421 tctttggttg cctgggcctg ctgggatgcg aggacgtctg ccccaagaag atccccctcc
    30481 aggatcaatt gggaatcctg cgaaggaaga tgggcttcag ctccatccaa cacctgttcg
    30541 gcaaggtctt tgccaggaag taaccggccc gggacggaca gcttccgtcc cgagtcttga
    30601 ctcgtgcgtt acaatacatt tgggaagata acctctttcc tcctcccgag agagccgggt
    30661 tcgcctcctc cttcccggct ctctttcttt tttcccgccg atcatctctc gaaaaaccgg
    30721 cccacatcct tgaccaccag ggcgggtttg ttgcgatacg catcccattc cacctcgaaa
    30781 atcacgtccg cgtcctcgag gctgccccat cggccggctg caaaggatgc ggcccccctc
    30841 cagaagacgc cttcgatccc tccgttcagg accagtctca ggtggctgcc ttcctttccg
    30901 aaggcccgtt gaacggcgac ccgggttccc gccgccgcaa acaagggact ggggtttccc
    30961 agtccgtggg gctcgaatcc ccggagggcc gccacaagct ccccggacac cagtcccagg
    31021 tccagcctca tatccaccct ttttctggga acgaagattt cggggtcgct cctgcgggca
    31081 acttcctcga aacggtccgc aaaagcgtcc aggttggccg ggtccagggt cactcccgcc
    31141 gccgccttgt gcccgcccca gcgctgcaga aggtcccggc attccatgag ggccctgtgc
    31201 aggtcgaagc cggggatgct gcgcccggat ccccgggcca ctccgttctt ttcgtcgatg
    31261 gacaccagga tggtgggaac gtgtttccat tcctgctgta tccgggaggc cgcaatcccg
    31321 acgaccccca caggccaccc cgcatccccc aggaccagag ttttcctgct ctcgtcggcc
    31381 cacagaagcc tccgtcggat ttcttccagg atctcttcct cctgctgttg ccttcgggcg
    31441 ttcaggcggt tcagttcccc ggccagggaa cccatctcct ccggagtttc cgacaccagc
    31501 aggtcgtagg cgattcgggc atgggagacc cgcccggcgg cgttgattcg aggaccgatg
    31561 tagtaaccga catgtcctgc cgacaggggt ttgcgatcca gtcccgccga ctcgaggatg
    31621 gcgcggatgg gtccgtgagg tccgcggttc aggctttcca tgccgcttcg ggcgatgatg
    31681 cggttgccct ccaccagggg caccaggtcg gcgatggtcc cgatggcggc cagctcgagg
    31741 tattttcccc ccaggcgggg ccgggggaac ccgccggcca aggctttgcg aagccgggtc
    31801 aggaaaagaa gggtgagccc cgaagagcag aattccttga agggagaagg gcaccccggt
    31861 tgcttggggt tgacgaggac cgtggcgggg gcggcccctt cccgagggac ctcgtggtgg
    31921 tcgaggacga ggcaatcggc cccgaggcca cggatccgtt cgattttctc cctttcggaa
    31981 gtcccgcagt ccatgaccac aaaaagcccg gcatccggat gatccggaat cgcccgcccg
    32041 ggaatgccgt acccttcctc ccggtcggga agcaccacgg agaagttatg gtaaccgatc
    32101 tccctcaaaa acagggacat ctgggcggcg ctcgtgattc cgtcgcagtc gtagtcggcc
    32161 accaagacca ctttgcggtc ctctcgaagc cggcgggcca tgaggtccgc cccctcttcc
    32221 aggcctttca cggcaaggta cgccttcagg tctgcgagct ccgaggacag aaaggaggga
    32281 ccggctcccc ggttttccag gaggattcgg accacgtctt ccacggagca gggctgctgc
    32341 tgcgcggcga tctcccattt ccactcgatc atcggcaccg gtcttccgtc ccgggatgga
    32401 ttcgccaacg gactgtttcc ctttcccccc gcccttcccg tcgagggagg aggaaggtcc
    32461 ccttcatttg gccagagagg aggggcggtg cttcgttggg ggcaggtccc ggttttgtgt
    32521 ctcgaaaccc aaggctgctc attttcactg aaatcttccc acctatcgag tacccatttt
    32581 gccaaaaatc tggtataagg accgccgttt ctttcctttt gacccttttt acccggggct
    32641 gaaaggttgt caaggcccgg gatttgcccc ggcaggggtg gaaagacccg aaagaatcga
    32701 gggaaggttc cggcccgtat acggaatctt ctcgcgggtt ttgcattccg tgccggggag
    32761 cgatatattg aggaccctgc cttgtggttt ttgttggagc ttttatcagg aaccgtgttg
    32821 aggacccaac gttgttgggt taggcatgaa gacatcaccc gtcatccttt caatatcccc
    32881 tgctcccagc agactcccgt tcccttgaga accgaaggta cacccttccc gtgggaagga
    32941 aggcacgaag ctggatccag aggcgttccg gaagccatag gagctgtcat catggacacg
    33001 tttacgtatc tgaagaattt cattcgtgac agaaacgttg cgtctattac tcccacctct
    33061 tccttttgcg ttcggaaggt ctgcaacaag atcaattttg accgaagcga gctcatcgtc
    33121 gaatacggcc ccggcacggg ggtcttcacc gactacctgt tgagacggat gagaaacgac
    33181 gcgcggctta tcctcatcga gcgcaacaag aacttcaact cgatcctgga aaaaaacttt
    33241 cacgatcccc gggtcaccat tgtgaacggc agtgccgaaa acgttttgga tgccttgaac
    33301 aacagcaagg aaaccaaggc ggactgtatc ttgtccggga ttcctttcat gctccttccc
    33361 cgggagctca agcacaggat ccttgccaac acccacgagg ccctcaagaa agggggcaag
    33421 ttcctggtct accaaacctg ttttcagacc gaaaaccacc tcaaggtgca ccttgaccga
    33481 tactttcccg tcgtccgaac caaattcgag atgttgaaca tccctcccct ccgagtttat
    33541 gaagccatca agtagcgatt cgcccggctg gaaggaacgg gacatcgaga acttccaatt
    33601 ccggcgaatc catccctccc ttttcatggg cacggccagc gatcgataca gcggctgggt
    33661 ggggcagatc tacacccggg agctctacga ggggcgaatt cgggcacgga ctcaccgggt
    33721 cggacccaac tcctttcggg aagaggtcct gccggtggag agcgtcgacg aatatttcca
    33781 gcacttcggc gtcctcgaga tcgattacac cttctacgac acgctcctat gcgcccgggg
    33841 aaatcctacg gccacccacc gaatcctggg agaataccgg cagcggcttt cgggagagga
    33901 cgcactggtc ctcaaagtcc cccgactcat tttcgcccgg aaaatcatgc aggcggggaa
    33961 acttcttcca aatcccctgt accttgaccc ggaggccttc gtccgcaggt tctacgaacc
    34021 ggcctgccgg ctcctgggaa accacctgag ggggatggtg ttcgaacagg aatatcagcg
    34081 ggcgtccgat cgggcctcgg tagatcggtt catcgaggag ttggacaact tctttcgttc
    34141 catcccccgg gacgaccgct accacatcga agtgaggacc ccccgatacc tggtttcccc
    34201 ggttttctcc ctttttctga cccacgggat cggacaggtt ctttcccatt ggacctggct
    34261 gccccccctc tcgaagcagt gggatctgtc cggaaggcgt ggttccaacc gcggcggcca
    34321 ggtcgtcttt cggctcatga ctccccgggg aatgcgctac gaacaagcct atgcccgagc
    34381 ccaaccattt gacaaactcg ttcccggcat gctacagccc gacatgatcg aggatacggt
    34441 ccggatcatc cgggacggat tggatcgggg gttgcgtgtc catgtgatcg tcaacaatcg
    34501 tgccggagga aacgcacccc ggatcgcccg gcggatcgcc cgcctcttcc tggcccggga
    34561 aaaggaacgg acccccgagg catagggcat ggaaagacgt tgtgcagacc gttccgcggg
    34621 gaggatcgga cggccgcgca ccggaggtgg aaacacgggg ttgccgggtt cgcccctccc
    34681 ggacgacgtt tctttcctgc cgccgactcg aaacgacact ccttccggca ctcgcgaccc
    34741 ggctttggtc tctgaatcac cccgtcgggg tacaaaacga ggagcaattc atgaaatctg
    34801 tggaaagact gctgatttgt gccgtcgtgg cttggaccct tggattccat cttgagcccg
    34861 cccttgccga tgaggtttcc gatcagatca aggaggccct gcaacaatac gaaaggggaa
    34921 atctctccga ggcggccaat tctctgcggt ttgccgtcgg ccagatccag cagaagctct
    34981 ccggggacct caaagacgtg tttcctccac cgctgagcgg ctggagtgcc gaggagacca
    35041 cgggagactt cggtgcggca tccttcctgg gaggtgggat ctcggcctcc cgccattatt
    35101 tcgtccggga ctccgatcga gcggtcgaca tcgagatcgt tacggattcc ccgctgctgc
    35161 aatccgtcct gatgttctac agcaaccccg ccttctatgc gaaccagccc gacgccaagt
    35221 tggtgaagat tcagaaccga aaggccgttc agaagtactc ggcccaggac aaagaagggg
    35281 aaatcaacct cgtcatcggc tctcgaatgc tcgtttccat caaggcaatg gggttggaca
    35341 agccggacga catggttgcc tatgccaacg ccattcgcta cgacgccctg gagaggttct
    35401 tggaaaagta gtcagccggg tccgcaaccc cgggagggcc ggtgctggga cggcatggaa
    35461 aagtctttct ttacacacgg accatgctac aatgccggga acaggtgctt cattgcaggg
    35521 gccaaaccat ggacggcccg gtgcgatccc gggggctggg ccggggggtg ccgcaaccgg
    35581 ccgagtccgt tcccaacatt ggttcgagat tgatttatgg ccaggacagc gatctgttcg
    35641 gaagacccca acgacctgct ccggacttct ctggagtacc agactcgtct gaatcagatc
    35701 tacaagcttc tccatgaagc tcacaatttc atggacgtcc tcccggacct ggagaaggat
    35761 ctcttgtccc ttctgagggc ggagcgcctg accatttacc aaaagggtcg cggagaccgg
    35821 gagatcgttt ctcgctacaa gacgggccgg gaaatcaagg aaatccgcct ccccctcagc
    35881 accacttcca tcgcggggta cgtggccctc acccagcaag ccctgcgcat tgacgatgta
    35941 tacgacagca aggccctggc ttccattcac gccaacctga cctttgattc gagcttcgat
    36001 cagagatcgg ggtttagaac tcgatccatg atcgtgctgc ccatcaagtt cggcgatgtt
    36061 ttgctgggtg tgctccagat catcaaccgc atcggcgggc cctttacgga ccaggacctg
    36121 gtgcacgccc gggaaatcgc ccgagtcatc gggcagaagt ttcgctacga cctgggcggc
    36181 accaaggggc cttacgacta cctggttcag accaagcgaa tcactcccga gaagctcgaa
    36241 gagtgcaaaa aggtcgctgc ggctcagaaa acgaccctaa ctttcgtgct catgaacgaa
    36301 atgaagatcc cggccgagga aatcggggct tccctcgagc tctattatca ggtcccctat
    36361 atgaagtacg atccggacgt ggaactgccc aaggcccttc tcaagaggct caatgaagcg
    36421 tacctgagga gccacctctg ggtacccgtc ggcgtcgacc aggaaaaggt gatcgtgctc
    36481 atcgacaatc ccaccgacac caatcggatc atggaaatcc agaaggtgtt gaatgcccgc
    36541 aactacgaat ttcgagtggg gctccccgaa gacatcttga agttcctggg agtggggctg
    36601 gaaatccccg agcccgaaag cgtgaacctg gaagacctgg tgggccgtct cgaagacgaa
    36661 gcggccgtga tcgccgacga gcaggacatc gccctggctt ccacggtgag tgaaaacgaa
    36721 gccaccgtgg tccagatcgt caatcgcctg gtcctggacg ccgaaaggct gggggcttcg
    36781 gacatccata tcgaaccggc ccgggggaaa gtgcccgcga cggttcgcat gcgcgtggac
    36841 ggagtgtgtc gtccggtgct gaagatcccc gccagccaca tccgggcggt ggtggcccgc
    36901 atcaaggtcg tctcccggct ggacatcgcc gagcggcgca aaccccaaga cgggaaattc
    36961 atggtgaagc tccggggcaa gaacctcgag cttcgcgtgg ccaccattcc caccgtccat
    37021 ggggaaggag cggttttgcg gttgctgcat tcaggggggg ccctgccctt tgacaacctg
    37081 aacctttccg agcgcaacgc ccgggaaact aaggaactgg tctcccaccc tcatggaatc
    37141 ttcctggtgg tgggtcccac gggttcgggc aaaaccacca ccctccatgc gatcctggga
    37201 cacatcaaca cccccgagcg caagatctgg acggcggaag accccgtgga aatcacccag
    37261 gagggactcc agcaggtcca ggtggaaccc aagatcggct ttgattttgc cgccgccctc
    37321 cgggccttcc ttcgagcgga cccggacgtg atcctcatcg gggaaattcg agaccaggaa
    37381 acggctcata gcgccatcga ggcatccttg accggtcacc tggtgttttc gaccctgcac
    37441 accaattcgg cccccgagac cgtgacacgg cttctggacc tggggttgga tcccatgaat
    37501 ttcgccgacg cccttctggg ggtcctggcc cagcgtctca gccgaaccct ttgcagcttc
    37561 tgcaggaaac cgcaccccgc ctcgaacgag gaggttcacc agctgatggt gagttacggg
    37621 caagagatgt tcccggagct gggggtgaat ccctcggaga tgattctcca gggtcctgcg
    37681 gggtgcgacc gatgcggggg tacgggatac cggggacgaa tcggcatcca tgaactgctg
    37741 gtggcaacca atgccatgaa ggtggtcatt gccaggaaaa gcggcgtgac cgagatccgc
    37801 aacctggcca ccgccgaggg gatgcgcacc ctgatgcagg acggtattgc caagatcgtc
    37861 cggggtttta ccgatttttc ccagctgcgg cgagtggtgg cctgaccgca gtccccttcg
    37921 ttcctcccac cggaaggtcc tcgaggtcct cgttcgacac gcagccccca tgccggtcgg
    37981 tcttcacgtt cgaggtggaa gcctcgatta cccgttcttc tctcccttgg ttgtccataa
    38041 gccggaccgg ggtcggcccc ggggccggag ccccgatgga acctcgggcg gcggaaggtt
    38101 gtgcgtatcg gggcggcgga taggccgcga cgggcgggaa ggctgtaggt ttttccgccc
    38161 ggagtcggct tcatctgcgg gttgcgcggc gcgaaacgtc cgcgacaacc cctgcgtagg
    38221 tctcccctcc tgcccccgga agcagttccc cccagcggag gcctccggga gaaggaaggg
    38281 cttgtcgtgg ttggaccggg accggacgtc ccgcccacgc cggcccccca ccggctcgga
    38341 cgagatccgg gagcaccgtc ttgatgtcct tgccgacctg tttttccagg ggattcgaga
    38401 gacggatcgg atcattcgtg cttccggtcc gggaccttgg aggcggttga agaagtgagg
    38461 gaacgaaccg acagagagga ggcgtggagc ctctcggcca cgtccctgta ttcgatggac
    38521 cggcgccccc tcggggaccc ggattttgct cacttcacgg atcagggcgc ggctccggcg
    38581 gcagacggtc acgggtacgg ccggtccttg ggagaacgcg gccgtagggc accggaaagc
    38641 cgcccctgcc gtcatgccgc ccgggattct tctgcaagcc cgttgccgga cccccctcgg
    38701 gaccgaatcc ggcgcacgga aacaggacgg aggatcgccc cgaacgcaga cagaggatcc
    38761 aaaggatttt tcgacagggg ggacccggga cccgagggaa cgcgtctttc cggaccgccg
    38821 actttattcc ttcttgtttt tgggcctccg gatggtgcgg gttcgcttgt ctcccgcgcc
    38881 ttcttctccg gacgccggct cctcggacga ggactggctt ttcttaagct tgcgctcctg
    38941 ctggaagcgc accagggcct cgggcagacc gcgcttcttg gccgcactgc tccgcgcccg
    39001 ggtaagcgac ttggccgaca gcggctgttt gagggaaaaa ccccacttct ttttgtattc
    39061 tctggggtcc aaatcatggg accctaaatg cttggtcgtc aactggcgca tttccgcacc
    39121 gcattccatg caaatgatct tgtcttcctg aatggagtct ttcgcctcca ttttctcagg
    39181 agccttctcc ttgacggatg gttcctccag ggccttcgcc tggtctaaaa gcaccccttc
    39241 ctcttcagcc ttctgcattt tctgcaaggc tccaaaagtt ttcaataaag ccagttcaat
    39301 ctgatcggcc gtcatttgac caaccgatgc ttgtgcctcc acaatgtcgg cggcaatctc
    39361 caagagcttt cgtgtcattg aatgcctccc tctacttaag aatcgaatac cggaaaacgt
    39421 cttagcccca tgatgagtcc gagttatgat gatatcgtag gaagtcgttc ttctcggaac
    39481 ggcacctaat gaattcaatc ggttagagtc gaagaaacgc actcctcagg actttttacg
    39541 agaccatcag ctataattat gtagtgtttt ctatatgctg tcaatatcaa cagtacgaat
    39601 aaaggcacct gtaagaaata gcaacctcgt ccttccaata acctgaacat ctcatgacag
    39661 gtggtccgtt tcacggctgt ttgcttttct ccttttccaa tcaagacagg acgacaacct
    39721 ggggcacgac ggcagtcgat ctctcggctt cattcctttt gacagagtcg gagggttggg
    39781 ataagttccc gaattgacct tatcccgtcc gtggtaaaca tggaggaggg gcggacttcc
    39841 gggtggagtc gaccttcttt ccgtttggac ggctctcacg gttaaaggat gagggagttg
    39901 gttttcccgg ttttgcccag ccccattggg gtgagatgcg gaccaattat atcgggaggt
    39961 ggttgccatg tggaaacgaa cggtctttgg gacagctctt ttgtgcctgc tctcagctgc
    40021 ttgtccattg gtttttgccc agaattttgc cgggacctat gtgcgcatcg gggacagcaa
    40081 ggaatatctg accctgagcc ccgacggcac cttcttcctg aaacagcgaa agaagccttc
    40141 cgacctggca aacccttttc aaaccatcac ggggcattac cagatccagg ggaccgagat
    40201 cattctgcgt cttcccggcg ggggggaagc cacgggaaag attcaggaga acatcttcga
    40261 ggactcggac gggaagaaat gggccaagga taagagcaag atcccccttc cccaagacac
    40321 ggacaggcgt cccacggaag gtcgcggcca gaggtattga ttaccaggtc cccggaaaag
    40381 ttcatgggcc aagagcctac gacccatggg ctcctcctcg ggacggtcgg cgggagggcg
    40441 acggctggga gggccaggaa gcgtgaatcc tggtccctgc gtctcggcgg attccacccg
    40501 aaaagacccg cccgagagct cgacccgttc ctgtatgctc ctcaacccta cccctgggtc
    40561 gcctccgggg gtccccagac ccccttgcgg gtcgggcctt ctttcatcat ccgtaatggt
    40621 cagggtcagt gcaccctcgt ctttctcgag aactacagag acccggctga ccttcgcgct
    40681 ccgggcgatg gtgttcatgg cttcccggat cacccggaag atcacgattt tcagggacac
    40741 ggaaattgcc gcttcttcca cggaaaaccg ctcctcgacg gcgatggtga ggtgggtatt
    40801 ccggaactcc ccgcagaacc agccgatggt ggcgccgatg cccaggtcgt ccagcacgga
    40861 cggccccaga cccgtgtaga tatttcgtac ctcctcgatg gggccccgga ccatcgggac
    40921 aagcggatcc ggtagctcag ccgcctccac tccatcccgt ttcaacagcc ggagaacgtt
    40981 ttcaatgctg aacttgaccg ccaccaggga ttggccgatg gaatcgtgca attccacggc
    41041 gatgcgctgt ctttcctcct cctgagtgtg aaggagcccc gggcggagag tgttcgatga
    41101 gctgttcgaa ccgctcctcg cttcttcgga gggcttcttc ggcctctttt cggtgggtga
    41161 tgtcctgtac cggggcaaaa tagcccggca cttgactgtc gggggccggg tggggaacct
    41221 attccgccgg aaagcatctg cgcacaccgc cggccggatg catttccccc tcgaaagaca
    41281 cggcttcccc tctcagcacg gccgtcacgt gttccatcac caatccgtag ggcccggccc
    41341 ccaggagctc cttcaccgac atgccggcaa tcctcgaccg gggaattcca aaccagcgct
    41401 cgtagtcctt gttcacgacc cggcaacgct ggtccctatt cacataggaa gatgatgggg
    41461 aaatgttccg gtttcatgga ttccttcctc ggagtgaccc gtctcccccg aagaccgaat
    41521 tcggaccccc cggtcccgcg ccggggaaac gacgggcggc cccgacaacc ggaactgccg
    41581 acgatgggaa aaaacccgat ctcccatgcc cctcggccgg gatcttcctc ccgagggggg
    41641 cgcgagttga gatcgcaacc caagagagga caagggcccg gaagaggaac ggaaccgaac
    41701 cggtctacgc cccttcctct ttcttcggca actggtccag gtgccattgc atgggattga
    41761 gcagggtgaa ccccagggcc tcgagcttct tcttcacccg agtcacgttg gtggtgagca
    41821 ggtacggaaa tactgcgcgc ttcccttctt cccagtgccg ggccacaaga atgcttccga
    41881 acgaaatccg ctcctcactg atggcatcca caatccgctt catctggccc agcttgtcct
    41941 ccaccaggat gcacaggagg gtgcccggtt cttcgatgcc caggacattg atgaaggcgc
    42001 gcatgagatc tcggatggaa aggattcctc gaagctttcc ctgcccgtcc accacgggga
    42061 aagcacccac ccgcgtccgc tggatcagca ggagggcgtc ctcgagggtg tgagcgggcg
    42121 agatggtgat ggggttcctc gtcatgatgt ccttcaccct cagttcccgg acccgttcct
    42181 ccattcccgc ggactcccct tcgtgcagga tgggcgaagg cagggcgctc cggatgtccc
    42241 ggtcggtcac aatgccgata aggtagttgt ccgcattcac cacgggaaga tggcgaatac
    42301 ggttttgcgt catgatccct ttggcctcga tgacgctgca ttcgggatcc accgcgacga
    42361 cattcctcgt catggacttg tcaatgaaca tctcctcctc ccctcatccc gccggaaaac
    42421 cgaatccttc cagcctgccc ggtgatcatc ccggtgttcg gtttcaatgt tccccctcga
    42481 cggaagggcc cgcccccctc catgggacga cggcccccgg gaaatgtcta gctccccagg
    42541 aggatctcca cgtggttttt caccaactcc ggcaagagat ccaacgaata ccccccctcc
    42601 aggatggaaa tcaaacgtcc ttcagcatat ttttccgcca acccgaccat ggtttccatg
    42661 agccatgaga acccctccgt tgtgagattc acgccggaca tgtcgtcctc gacgtgcgca
    42721 tcaaacccgg cggacaacag gatgaccagg gggcgatatc gatcaaaggc ggggatcagg
    42781 tccctctgaa tgagctcccg gtattcctcg tcgccctgtc cgggcagaac cgtggagttc
    42841 agagtatagc cgtacccgaa gtccgtccct cgttcgaact cgcgacccgt accggggtag
    42901 gcaaaagagg ggtgttcatg aatggagtag taaaacacgg ttcgttcggt ttcgaaagcc
    42961 ttctgggtcc cgttgccgtg atggacgtcg aagtccacga tgccgatcct gggaactccc
    43021 cattgcttct ggagatacct ggccgctatg gcgacattgt tgaaatagca gaaacccatc
    43081 gctttgtcgg cctcggcgtg atgccccgga ggcctcacgg cgcagaaggc gttgtccagt
    43141 ctcttctcca tgaccagcct cacggcgttg aggacccctc ccaccgccag gagtgccgtt
    43201 tcaaaggtgt ctttgcacat ctggttgtcg gggtggtcga attccgaaac cccgttgagg
    43261 caggcatccc tgagacgctc gatatgctct cggctatgca ccgtttccac ccacttgagg
    43321 tcggcgggac gggcttccac cagggtcagc ttcggcaaaa gtccggcctg ttggatccct
    43381 cgatagatgg attccaggcg ctcggcagcc tccggatgac aaggccccgt caggtgccga
    43441 aggtaccttt catcgtaaag aaatcccgtc cttttcatac cagccccttg cggatggaac
    43501 ctctggagga tgtcccgggt caggtcgcaa gaccttgggt cacttccgct ctcctatgct
    43561 cgttttcata acacgtccca gacggagttg caaagagtcc gtccgcggga gatcccggga
    43621 acgggagatc cgtgcggctc ctctccggtc cgattccctt gaatccgcaa acaaagtgcc
    43681 tatcttcaag ggcattccgg cggagccgag ggatcaagag aatttccggg atcgccctga
    43741 gaggatgggg gcggttggcc cggcaggccc cccgaactcg atgtgcctgc aggagcgcgg
    43801 ctgtgctgtg gcacggatgc ccccgcaggt gttcacggca ccccaaacgc gtcctcgctg
    43861 acgcccacac catgaggagt cccacatggc aagactgctc catatccagg caagccccat
    43921 gggtgatttg tccttctcca atcgattggc ggccgctttt cttggttcct accgggcagc
    43981 gcatcccaac gattccatcg aacagctgga cctttggagg gaagatctgc ccgctttcga
    44041 tttcaccgcc gcctcgggaa aatacaaggt tctgcggggt ctggaccact cccccgaaga
    44101 agcccgcgca cggcagcggg tcgtagagct tacggaacac ctcaagtccg cagacaaggt
    44161 ggttgtttcc agcgggatgt ggaacttttc cattccctat cgtctcaagc aattcatcga
    44221 caacattgtt caaccgggcc tcagcttctc ctacgacccg gtcaagggat acagcggcct
    44281 catcacggga aaacccctcc agatccttct ggcgtccggg agcgaatacc cggcggggtc
    44341 cgatgcggcc gccatggact tccagaagcc ctacctggaa atgatctttc gattcatcgg
    44401 tttcgaggac attcgaaccc ttcgagtgga ggggaccctt tccgcccagg cggaagccaa
    44461 cctcgtgaaa ggaatggacg cggccgtgga agccgggaag aatttctagc ggggcagtcc
    44521 ccccatgacg gcctttaccc cccattcttc ttctcacgcc ccgtcccttc tgctatcatc
    44581 agccgatgaa tccttcttgg atcattagcc cgggaggagg ggacacgcag acgctcaagg
    44641 gatttgtgga gggggccttg gtgatgaagg ttcgtttctg gggaagtcgc ggttctctgc
    44701 cggccccact gacggcggag gccgtccgga agaagatcct ccgagccctg agggattcga
    44761 ggaatctctc cctggacaac gacgaggatc tcgcccggtt cgtggaagca caactgccct
    44821 tttccgttcg cgggacctac ggaggcaaca cctcctgcgt ggaaatcgcc ggaggagatg
    44881 aggcggtcct gtgtgacgcg ggaacaggca tcagggtctt cggcaaccac gtgctgaggg
    44941 agagcgcgaa ccaggggagg aaacctcccc gggtctttca tctcttcatg tcccatctcc
    45001 attgggacca tctacagggt tttccgtttt tcgttcctgc ctacattccc ggcaacgcga
    45061 ttcacatcta cggtttccac gaacggctaa aagaggcttt cagcaatcag caacaagggt
    45121 tgcatttccc catgcccctc gagaatcttc tctcggacat ccggttccac gtgctggaga
    45181 ccgacaagga ttacgccatt gccgggttca acgtccgggg agtcaagcag aaccatccgg
    45241 gcggctcctt cgggtaccgg ttcgaacggg ggggaaaggt ccttgtttat tctaccgatt
    45301 ccgaacataa ggccgatatg gaaacggacg ggtatgcctt cctgagcttc atccaagacg
    45361 ccgatctact catcttcgat gcccagtatt cctttggaga acaggtgtcc aacaaggaag
    45421 actggggcca ttccagcaat atcatcgcgg tggagctcag cgtgaaggct ggcgtgaagc
    45481 acctttgcct tttccaccac gatccggtac tggacgacga tgccctcgac cggttcctca
    45541 cggatacccg cgaatacctc gatctctacg ccgggtccca tcccctccgg atcgacctcg
    45601 cccacgacgg cctgaccatc gacctgtaga gggctccggg acattcccgg tttccgggtc
    45661 tcgatgagtc tccccgagcc ccgggcgatg aaccgtttgc ctgggaggat tggttcaata
    45721 tctccgcggt tggcatcgag cgatgcggca ccggccgagt tctcgtccct ccttttgccg
    45781 gttgtccgag acccccggcc gacagtcggc ggagcatcgc tcatctcggc gtggggggtg
    45841 atcatcttcc ccgaaggaac gtcgttcgga accgggttcg ctccttccgg gccggggaag
    45901 gttcgtagac gaatcaagtc ccgggatcgt ctatgagttg aaaggtcttc gaacagggct
    45961 ttcaaagccc tccggcgtca tttccttgat ggtacaaatt caggagccta cgatgaaacg
    46021 attgcccggg agtttggttg cggcagtggt gctccttgtt cctttgttgg ccgggtgggt
    46081 gccggccgcg agtgcggaaa acggccctcc cctctcccag cctctggttc gggagggcga
    46141 ccttgcgatg gatcttgcct tggacctggg gctctctgtc gaaagtgagg ccgaagcgga
    46201 aagcaaactg gcctccatgg ggatcgaacc tcggaacgga tggatctcgg actacccggt
    46261 caccccggac attctcagtg agatccacga cgccgttgga gacgcagccg attcggggaa
    46321 gctcggaatg agcaagggcg aggccctggc tcttctcaca gacatcagca ttgattacgg
    46381 tctgtcggtg cgcactacct cggacggatc cccaccgccc gtccgggaag cggccgtgga
    46441 tccgtactac aacgatccgt ccctcatcaa cgactattac accgagtacg gccctgcggt
    46501 ggtgacctac tacaccccgc cctgggatta ctattccctc tacagctggg tcccctaccc
    46561 gttctggtgg agcggcatcg gcttttccgg gtacttcatt ctgaacgatt tccatcgaag
    46621 agtcgtggtg gtgaagaagc gggtcggcca tcccaagcac tggcgtcaca aaggccggtt
    46681 cgaggaaggg aaggtgagga cggtatccaa tcgcgtggtg gaccggaaca cgggaataac
    46741 ggcgaggttg gacccggtca cccgcaccat cgaccacgga ggccgatccg tacgaaacat
    46801 ttccaaccca cgttcgaggg cgggcctcga aagcggggac gggcggaggg acagcgcccc
    46861 tgtgccggag ggaagcctcg ggaggccgtc ccggggtaat gccggagccc ggggaaccgc
    46921 tatcccggat cggtggaacc gtcccgcgag caaagagtcg gaagcaggga gggaactccg
    46981 acccacccgt cgtcccaatc cgtccggaaa cagggagcgt ctgcgctcca tgcgtccgaa
    47041 aggcccctcc ttcgctccca atgggcggcg cagtgcgtcg tccggctcct ttcggcaagg
    47101 attcgctccc cgttttgatc gtcgatcctg gagcggaaac ggaaaccgag ccttcggcac
    47161 tctccagtac cggagtggat ccttccgggg ttccggaggc ggaggcaaca tcggtccctc
    47221 catcggacga ggcttttccg gcgggaattc ccggattggc ggaggcggag gcttttccgg
    47281 tgcgggccgt gggggattct tgggagggtc ccgcggaggc ttttccggcg ggcacggacg
    47341 ctcgggcggc ggaggtcgca ggtaacggtc ggttcccaaa ccttgaagga gggggcctag
    47401 ggcgagaagg tggtggattg atgggatgat tcttgcccgg gtggggttcc tgagcacctc
    47461 cggccttgcc ggagtgagga tccggcagag ggctggacgg gaccgttcaa cgtccggggg
    47521 gacgatacct atgtgaacct tgcggccgga cctaataagg cgtccccttg tggagtgaat
    47581 ggcaagggaa tggggagttc tttctcgagg aaaggatcga gcaaccgcaa gccgagttgg
    47641 aacaggctga gatcgcaccg tccgccgcag tagatgcttg tcccgaccat gccgcagggg
    47701 tatatttcac gataaaaaag ttgcgccaaa gatccctctg acacgctgag tgaagatttc
    47761 gggcaaatgt ctgaatcctg tgggtgggca gttcccgtgt gagtgcaact tcctattgat
    47821 ccagggctag gcgagacgag cagccatcag gggccggcac acaaaggggg gatcggaggt
    47881 ataaaccgca ggcgaaatgt cgtttcgaaa gggggggctt atgactagca ttcatagaaa
    47941 tgttggctta agaagtgttt gggtcgtcct cgtacttttc gggactattg cgctccctgc
    48001 tctttcccag cctctgcatg cccagacgtg ggggaagctc taccatggac catggactga
    48061 gtggtatacc ggcacgacct tttatggggc gctgcgcacc tctgacgggg gatacctgct
    48121 gacgggaact tcgaccgaac ggggcgtgat cctcaaggtt tcgaacgatg gaacgctgca
    48181 atggagcaag ctggtcaagg gaaacgggta tcagagagat acctccatga acggcgcagt
    48241 ggaggcacct aacggtgatt ttatcgtcgt ggggcaggcc tacacggaca cgaccatgta
    48301 tcccgaagcc ggagccgggt cgtgggacaa ttacatcatt cgcctgacat cgaccggcag
    48361 caaggtctgg gaaaagtttt acgctcgaaa gaacgtgggc gaagtccccg acacaaatga
    48421 atacgcgagg tccatttatg tggactgtcc ggaaggcggc gccccctgca gatacgtggt
    48481 gaccggccac gtcgtttacc attgtgtgcc gggatgccct tactacggct atcgtgcttc
    48541 tcagaccatg gtgatcgacg aggatgggga ggttcaggac gacttcctga tgggaggccc
    48601 gagttccacg ggcgatggag cggttcagac cgtctacacg ggcgacggca actaccttgt
    48661 tcttacgcgc ctgtggcatt cctatggcgg ctataccatc ggtcttcaga agtacggacc
    48721 taccggctcc ctgatctgga gcagggcata ttatgaagca taccggcagg aagacggaga
    48781 acatcttcta acgataactc cgggagcggt tgtggaaacc gaaaacggat atgtgcttgc
    48841 cggcttcagc tacgataatg tcactcaaga tcctcctttg ggaacctact accatttcca
    48901 agtggataag accaccggat cgacggtggc gtggaacaag aagtaccgag tctgggagaa
    48961 ttggtggagc tataatgctg gaagtcttgt gaccactccc gatggaggcc tccttgcgaa
    49021 ggtggaggat atcattacaa cggaaaacga ccgaattttc aagacctccc agaatgacgg
    49081 caccaagctg tgggtcaacg aatacggggc aaaccttggg accattaccc ccggagccgc
    49141 agacctcgca atcaccaggt ccgcctttga cgggctcctc gcgagccttt ccatcaacgg
    49201 cctcatggac gaatcatgca aggcactccc catttcggac aagaccgacg aggccgtgct
    49261 ttatccgatc ctgcggaccg acgtatttga tagcttcggt ccgagcgact gcaacagcgg
    49321 gatagggaag gtagccgtgt atgaggcgga tcgcatcgaa ccgggcgaat atcaacaggt
    49381 caacgcttgc acccctcacg gcgaagtcac cgctgactca aacggtcttt tcgggaaagt
    49441 gaatgtcggt tcaccgggca cggacaggaa ggtttacctt tcgaactcca acggcctcag
    49501 caatctctat gtcaataagg ttcttctgtc caacacgagg gatttcagta ttgtagcggg
    49561 aagcgacacc tgcaccggaa agacgcttta cccggtgaat gccggcactc cgactggatg
    49621 cgaggtgacg gttcggtttg cccctgttac ttatggagcc aagcaaggtt tggtcaagtt
    49681 tgattccaac gacgagactc tgctccagca ggtgctgaag ggaacgggtg tgggaggagc
    49741 tgtcaaggtg atcatctcac ccaaggcggc ccggaacgca ggggcgaggt ggagagtcga
    49801 cggcgggctt tggaagaaaa acggggactt ggtccagctc tcgaccggca gtcatgagat
    49861 ttccttcagg aaggtgaaag gttggagaaa gcctgataac aagattgtca aaatcgtcaa
    49921 cggggagact ttgcaaatcc gtgccgagta caggaaagtc gccaggtgag gagacagcgc
    49981 cggggccgag attgtccgtc gtaggggacg gtgcgctctc agcatcttcc gacttggcgt
    50041 gcgattgctc gatcatttcc tcaacgaaga acctaccgtt ccctccgcat cctcgccata
    50101 ggggaagccg ctgggatgtg tccgggtagc aaagggaacc ataaggcgcg ttgccacgtc
    50161 ccgctgaaga ggcgacccgg tcacatcccc agtcccccgg cactttgaag ggtccttctg
    50221 cttccgactg cagatgcggc cgggcgaatg ccgcaaaccc cgggtggttt gattcaccgg
    50281 gctgccgggc atgccgtctc tcgtgccgaa gaggtccgcc gggccgaacc cggcattccg
    50341 caagccgaaa tccggggaga ggatccggga ccgaaaactt tttcccgggg ggtgccgctt
    50401 ccatccacaa gccgggctag accaaaatgc accgcacgga tatccttggc tccggggaaa
    50461 tgctacaatg catcggtcat tgtcaccgac gcagtgcacc gctgcaaagg aggaaagtcg
    50521 tgcccaagga caaacccaaa agaggccctt acgccaatga gcgcgtcaac cctcacgagc
    50581 cccacgagat caaatactgg acgaagaagt tcaactgcac cgacatgcaa ttgaaacacg
    50641 ccgtaagggc ggtggggact tccgccgatg cggtgcgtca ctttctccag ggcaagaagt
    50701 gaggggcaac cgagaacggc gtgatcctct caattcccgt tggagaccag gacatatacg
    50761 tcgggagcca catcgggcag gaattgggcg aggtattcct gcctccacgc cccctccctc
    50821 cgtatttcgc ccatggaagt ctttgagagc agcaagaact tcttacccgg ccctgaaagc
    50881 acccctgcga cctcttccgg acgattgatt tctcgaaccg gccgatcccg gtaaaaggga
    50941 atggaacccc tcacatgatc cttgggccgg tagagataga ggggatgatc cccgacctcc
    51001 cgggacacga cctgcgccag tgtcaggtag caccgttctt tcttggtcat gtaaagcacg
    51061 ttgggcgtgt acagaatgat atgaacgctg gtcaggatcg cgatgcccag gcaaagaccg
    51121 tcgacgaatc ttttccggaa aagcagccgc gccgccgccg ggagcgtcag gctcaggagt
    51181 cccagggtgg cccaaaggta gcgcaccggg tccgcatgag aaaaggcgaa gtgtagagcg
    51241 gcaaagaccg ggatcatccc gacggcggcc aggccgaccc cttttgtcag ccttccctcc
    51301 agcggttttc catcgaagag tttcttgtgc agatagtata ggccaccatg accgcgtagg
    51361 ccgtgtagga aggaaggatg tagcttctct ccttgattgc gggtatggag agcaggatcg
    51421 gcgggacgaa cgcccagatc aacagaaaaa gaaagaattt cctcgtctcg ccgaaccctg
    51481 aacgcatttc ctttgccatt tcgaacagcg caaagggcaa gaagaggagc cacggaccca
    51541 ggatttccgg aaggacctcc aggtaaaaac tccagctttc cttgttcccc aggtcggtat
    51601 gatggaaccg gacctcctgc aaggcaaacg ggatgtgaag aaatcttccc aggatgttgt
    51661 tcacgaagtg ttcgtggaga taccccaacc ctccgtcccg gtaaaagagg aagacccaga
    51721 gggcgacggg taccaggaag gtccccgtca cggcgggtcg aaaggcgctt cgaaaaaact
    51781 ccccccttct ctccaccacg caaaaggccc caacgacgac cacgacatga aaaacggcta
    51841 tggtgccctt ggtgagaaag gcacaggcgg aaaagaaggc aagaaaatac aggagagact
    51901 gacgggggat ggttcggggg ggccaaaacc tccagagggc aaagctgacg atggccaggg
    51961 agcaaaaggc acccacggca atatcgagaa gaatccagtg gctgtagcgc cagaacttgg
    52021 gcatggtcat gagaacgaag gtcgccaacc atgcggcttc caggcccttc caccggtaca
    52081 caagaaccag ggttgccgcc aacatgacca gccccatcag gaccgatacg acccgcgccg
    52141 acgtagaggt gaccttgccg ttgagggcat aggatagagc gaccagattg tagtacaggg
    52201 gcggtttctc caggaaagga agcccgcaga ggtggggggt gatccagtgg ccgtcctcga
    52261 gcgtctcccg tgctatctcg gcctcccggg gttcgtccgg cccccagagg cagaagtaat
    52321 ccacccccgt cgacatcgct gtaagaacga cgaccgccag caagatggag atgccggtcc
    52381 cggcccggaa aactctcagg cgcatatcct acccgccctg gaaatggggg cgaggctttc
    52441 tcgaccccca cgccgttgcg gcgtgttccg cccccggatg tcgggtctct cgggtattcc
    52501 cattcgtcct cctgggcata acgcccacaa ccgcaaagag ttcccccggg agagccctga
    52561 accggtcatc caacgctccc gcttcccccg ggcaaaggct ccgtgagagt agcgcaattc
    52621 ctcgatatca acaagccctg gtgagcccct ggagagtcgg gaggaattgc ggagtgaaaa
    52681 cccaagcaag ggagccgcgg ggaatgggcc ggggaggcct tctcccctgt tttccctgaa
    52741 ttttccggac ccgtgtcctc gaggcacttt cggcctcggc ccatcggccg ggccggtggc
    52801 gatgaaacat gttcttgaca ctctatctag gatatgttat taatatcagt aaaaatatca
    52861 ggatcataca cctttgtcgt ctcactcgag gatcgctgcc gggccgctcc atcgagggga
    52921 aggggtcctc ttatcgaggg aggccatgaa aaggtacctt tctaaaatgc cttcttgatg
    52981 cccccatcga atggttcggg agtttcgatg ggggcgtcgt atttcttggc cggaaatgtg
    53041 ccgcacaggg tcccggccga acccggaccg gggtaaagga tggcggttca cttccaagga
    53101 cgtgcacgga aggagttcca catgaggagc cggccatgaa aagaaaatgt gctttcgctg
    53161 gattgatctt tgctctcatg ctgatgaccc tgctgggagc gggaagggct gccgaccgga
    53221 aagtcgttcg tgtaaccagt cccggcagca tgttcggcag gatgcatacc atctccgccc
    53281 tgttcagcag ggaccaaccc atgatccaga tagactttgt gcggagcgcc gatgtggaca
    53341 cgagtttctc cgcactgata gaaagatcca ccgatatcgc cctgaccact cgcaggataa
    53401 cccccaccga ggatcagttg gccaagagcc agggcatgga gcttacgggg cgtgtcatcg
    53461 gtcatggggg aatcgtcctc ataactgcgc ccggaaatcc catcgatggg ctcaccttgg
    53521 accaagtccg aaaggctttc acgggagaat gcaccaattg gcgtgagttg ggaggatccg
    53581 acggacccat cactgtggtc cgggtcggag aaaactaccc gggcaccaac ttcttcatgc
    53641 aggaagacct cttcgggggc cgccctttcg cggccaacgc cgtcgtagtg cccgagttca
    53701 acaatgtcct tcgcaaggtg gcccagagtg caggctcttt gggtttcgtg agaattcgag
    53761 atgccttcga gtctcccatt ccctttgaga tggccgtcaa ggtcttgaag gtgaggaagg
    53821 atgccgcttc cccggcgata caaccttccc gggccaccat cgccgattcg agttatccta
    53881 ttctgcgtcc ctattacgta tattatgaaa gcaaggcgga tacgagcatt gtgaaatacg
    53941 tcgagttctt ggagaagaag ggctgggggc aacaaaacct tctgtaggga ccctggaaag
    54001 gggggttctt tgaaagaatc tcgggaaaag ggggtggcga ggcgagaatg gccgtcataa
    54061 cgatatcgag gcagccgggg gccttcgggg aagaggtggc cgctcgggtg gccggggttt
    54121 tgggattcct gctcgtggac agggcacgcc tcgcccgatt ccggcaagag atgggccttc
    54181 gggaagacga ccccacaaac gcgcagaagg ggaatggagg cgaagaccgc tcggcgggtc
    54241 ccgaagacga gacctgtgcc cgcctgctgc cggacattct tgcccaattg gccgaggaac
    54301 acgatttggt ggtattgggc cgggggggac agggcttgtt ccgcaatcgc cccgggaccc
    54361 tgcatgtgcg aatcatcgca tcgcgtgcct tccgcattcg tcaggtgcgg cgccgcaggg
    54421 gcctgtcctc ccgggaagcc cggcgcagcc tcacggaact ggaaacccga cgcgccgcct
    54481 atgtgcgttc cttgtatcgc atgaactgga tggacccggc cctttacgat ctgacgcttc
    54541 gcatggatcg gctctccatc gaagaggccg tgaacctcat cgccacggcc gccgagacaa
    54601 tgaagcttcg gcaggtaccc agaaggctca tcgtcgaaga ccttcttccc gagacagaag
    54661 aacggaagaa cggggatggg tttgccaacg ataccgaaag agaatttgcc cgttttctgg
    54721 aattctatcg catcccttat gagtatgagc ctcgcacgtt tcctttggag gtcgatgccg
    54781 ggggcagggt gctggaggcc ttcactccgg atttctacct gcccgagcag gaccttttca
    54841 tcgagctcac taccatgaaa caaaggctgg tcacccgcaa gaaccggaag atacgaaagc
    54901 tgcgccgact tcatcccgag gtgaacattc gcatcttcta tcaacgggat ttccacaacc
    54961 tcttggcaaa attcggtctg ttgtggggaa cttcccctgg aaacgccgac acgccggacg
    55021 gccaatgagg gagccctgcg gccccgggtc gaccggcggg cgttgaggaa cgcgatgcat
    55081 gacaacccgc tttgaccaac atatcaccga ggttctgatc ccacaggaag ccatcgcccg
    55141 gcgcgttgcc gccatggggg aagaggtttc cgccgccttc gaaaactcat gcccgctgtt
    55201 ggtcggagtg ctcaaaggga gcgccctgtt catggccgac ctggtgaggc acatgaaaat
    55261 cccgttggag atggatttca tggccgtggc aagctacgcc gatcaggaac agcgggtatc
    55321 cagcggggcg gcgcgcatta ccaaggacct cgataacgtg atcgaggggc gggacgtcct
    55381 cctggtggaa ggcattgtgg acacggggat gaccgtcggg tacctccttc ggagcctcga
    55441 ggcgaggcag ccgcgttcga ttcgtttgtg caccctgctg gacaagcccg cccggcggat
    55501 cgtccaggtg cccatcgcct tcagaggctt cgaaatcccc gaccggttcg tggtggggta
    55561 cgggcttgat ttccgccggc tctaccgaaa tttgccctac attggggtac tgagcgggga
    55621 agcgacgggg ctgaatcgca gctgacaaaa agtttcgcct tcgaagaaat tttgcttgac
    55681 atcctctccg cctttggata tggtcagatt gaaccccaag aggttgaagt agggaacccg
    55741 ataaaaggct cggaagggaa gcttggcgga aaccgggatg tccaatcccc cgggggaagt
    55801 tgggcggaag agtcgggatg agccggaagc ggcgaagtcg ggggaccgaa aaggtcactt
    55861 ggggttctcc tttttccaaa cctccaccag cgatccatcc cctggagcgc cctaaccgcg
    55921 ttttgtgccg cctcgggacg ggcgacatgc cggggacgcc cgatcggcac tctgcggccc
    55981 cctctgcaac gccttgggag tccggatggg ttcgttccgc gcaccgttca ggcagattca
    56041 gcttttccca tttcttgaca gctggtaata aagaccgatg gggacccatg caatggggtc
    56101 ccggcagccg cgccgtcggt cgagggcggg agggtgcgcg ggcaattttg cgtaccgacc
    56161 gagcggagcg aagccgcccg cagggcgggg gttgctcgaa cgcccttgag aagcggagcg
    56221 cacgcgacgc ctgaaagggg gttcgagcag ggactcaaca gcgtgcgatg gaggtctaca
    56281 aagaccctgc tcggcggaag gcctgaaagg gggttcgagc agggactcaa cagcgttgat
    56341 aagcggagcg ctcgcggtcc tcttatcaac aggacgtatg ggatctcacg ggagcgggtt
    56401 tccccatcga gacggaagat gggcaacggg agatgtttgg ttcggatggc cgggcatctc
    56461 ggcacagatg gaaagtgaga agatgggact ttgccgtttg cccgataccg tcttcgtgac
    56521 cttggcgacc ttcgtgcttt tctcgttctt cgggttcttc acggcaatga tcgcgggacg
    56581 aacgcccgca ggccttcgcc gacatggccg gccgcgggac tcttcgcccg gcaccgtcgt
    56641 accgggcctc ctcgcaggaa cctggatttt gctggggggg ctcggcttct tgcaggcaga
    56701 cgcagcccag aacggagatc ccatccgggt gggtgccccc attccccggg ccagcaacta
    56761 cgggcaaaac ggggaacggg gcatgatcct ggccgtcgag gagatcaacg aatccggggg
    56821 ggtgaaggtg ggcgaccggc ggaggccctt ccggctggaa atcatcgata cccgcgatga
    56881 ggagccgggg gtgcccacca gcgaagtgct gcttgccatc gaaaagttga ttcttcggtc
    56941 caaggtggac ctggtggccg ggggcccctg catgtcggaa tgcggcatgg ccgccctgga
    57001 tctcttcgct cggtacagga agctcgacct ggtcagcatc gggtgctaca cccccagctg
    57061 ggacgaaaag gtttcccgag accggcacaa gtaccggttt tcctttcggg agagcggcag
    57121 tgtccggtgg tacgtcaggg aggccctcga tcttctccgc aggatgaaag agaggttcgg
    57181 cttcgagacc ctctttgtgt ccatcgacga cagcctgatg tgccgaaggg cctcggagat
    57241 cgtcgaaact ctggcgatca aggacgggtg gaaaatcgtg ggcaaggacg tgcatcccat
    57301 tgcttccacc gactattccg cggctttgac cgagtgcagg aaatccgggg cccagattct
    57361 tttcttgtgg gcctattccc cggagacctc catcctcatg aagcagtggg ccaccatgga
    57421 ggtgcctgcc ctgcccgtgg gatttatcgg cgcggcggaa gaccccgggt tctgggaggc
    57481 caccgacggg caatgcgcct acagcatcgt gacgctctcc gaaacgggaa acgtgcccac
    57541 ccgggccacc ccgaaggcca tggaattcca ccatgcgttc cgaaggcgct ggaacgtgcc
    57601 tccccgaagc acgggatgcg tgagtgccta cgaggccatg tacgtgctca aggacgccat
    57661 cgagcgtgcg ggcagcctga aggaagaagc cttgatttcg gccctcgaga agacgtggct
    57721 ggaggcggtt cgggggacca tccgtttcga cgagaaccac caggtcgttt acggctacga
    57781 tccccgcacc agcgtactgg gatgctgggt ccagtggcag gacggcgaac gggtccaggt
    57841 cttcccggat gcggccgcca tgggagagat caggcttccc ccctggatga agcgctgagg
    57901 agaagccgga gaccctgcac gacaccgggg tcctccatgc cgggagatcc tccatggagt
    57961 cggttctcgt atacggaacc atcaacagca tcatcttcgc tctgaacgcc atcggcttta
    58021 gcctggtcta cggggtcagt cgcgtcccca acttcgccca cggctccatt tacgtcctgg
    58081 cgggctttgt ggcttggtcc ctggtcaacg acctgggact tccctaccct ttggccgtcg
    58141 cgatcgctct cgccttttcc gccgtgatcg gctgggggat ccatcggctg gtccttcgcc
    58201 gcctgagggg tttgcccacc tccgaaatca ttgcctcctt tgcggtgggc ctcatcattc
    58261 tcgagggcct gcgtctgcag gggatcggcg gcttcaaggg cttcatcggc cccaattacg
    58321 tactgaaacc gtttgtggac gggaagatcg aggtcttggg agtcttcgtg gacagccagc
    58381 gcctgctggt ggtgggtgtg ggactggttc tggccccggt tctttgggcg ttcacccacc
    58441 acacccggtt gggattgtcc ctgagggccg tggcccagga cgaacaggcc gccatgatgc
    58501 tgggggttca ctcggaccgg tccgccctga tcgccatggt gctgggttcg gccctggcag
    58561 gcctggcggc ggtgctcgtc cttcccctgg gcaacgtcac ctgcgaagtc ggttaccagg
    58621 tcctcattta cgccctggcc gtctgcatcg tcggaggcct cggaaactgg gcgggcaccg
    58681 ttttggcctc cttcctgctg ggcttcgcca tgaaggtgag cacccactgg ttcggggccg
    58741 tctgggaatc ggtggtcctg gtgggcacca tcatcctggt cctgctggtc aagccctcgg
    58801 ggctgctggg caagcagaag gaactcgagg aaagggtgtg atgtcgaccc gaaagcttcg
    58861 aaaaaggagg gtggaccgag gcattcacgt ccgcacccgg gggctctacg ccctctcttc
    58921 gtaccgggaa atggcttatc ttctggcccc gcgactgttc ctcctcgtcg ggttgctggg
    58981 actgcctccg gcattggacc tttacgggca gcgggtgctg tgcgccgcgg cagtctttgc
    59041 catgccggcc ctggccttcg atttccttgc cgaatacgtc gggctcgtct gcttgggggg
    59101 agccctgttc atcggctttg gaggctatgc ggccgggttt ctcaacgccc gattgggcct
    59161 gtcccccgtc ctgaccattc ccctggggac cctggcggga gcctgcctga gcaccctggt
    59221 aattctgccc tgtttgaggc ttcggggagt ctactttgcc atggtcacgt tcatgatccc
    59281 gctctttgcc cggcatgtca tcgtggccct ggggcttttg gggagcaccg aggggattac
    59341 cggcctggac acactccccg accccctgat cgagatttac gtcatcctcg cggcggtcct
    59401 ggcaagcctc tttgccctga gacggctggt gaacgaggat ttggggctca tcttgagggg
    59461 catcaaggag aacgatcagg cggtggccgc ttccgggatc agcatcttgc gctaccgggt
    59521 cctggccgtg ttcatcgcct cttgcatcgg gtgcttcgcc ggggcttacc tggcccacct
    59581 ctacggctgg ctggggctgt ccctctttgc catggacttt tccatcctgc ccatcgccgc
    59641 cgcagtcatg ggaggcacgg gcaccttggc gggcccggtg gtcggggcat ttctcctgac
    59701 ttccctttcg gaagcattgc ggggtttcgg acaactgcgg gtggttctct actgcctggt
    59761 cctcctcgcc tttatcctct acaagcccga aggcttgctc aaggtgctcg agcgcaaata
    59821 tcagcagttc gaacgccggg aggaggtctg atcatggcag cgggggttct gctcgaggca
    59881 cgcggtgtga ccaaattctt cggaggcgtt cgggccgtcc gggacctcga tttctccctc
    59941 gggcaaggag aggtcctggg ggtgatcggg cccaacggcg cgggcaaaac caccctggta
    60001 aacattctca cggggttcgt ccggccggac cgcggtcggg tcgttttccg cggaaaggac
    60061 ataaccgggc ttccccccca caagatcgcc aaccgaggcc tagcccggac cttccagggg
    60121 atgaggccct atccggggct cccgacctac aagaacctgg tcgttcccct gaactcgccc
    60181 cgggttcgga agaccgcggc ggggaggttc ggggacctgg actcggtggc cctggatctt
    60241 ctcgaggagg tgggtttcga gcgggattcg tccgtgccct acaagaccgc ggactccctg
    60301 cccctgggct accagaaaag gctcgagctg gcccggtgca tcgccctgag gcccgaggtc
    60361 atcctgtgcg acgaggtctt ctcggggctg agcatggcgg aaatcagcag catgctgccg
    60421 ctgctcgaaa agctcctttc ccgggggatc gccctgatcc tggtcgagca ccggttgcgg
    60481 gagctcttca ggatcacccg ccgggtcctg gtcatgcgct tcggggaaaa gatcgcggaa
    60541 ggtcctcccc gggaggtcct cggtcgcccc gaggttacag cggccttcat cggcagagga
    60601 agccctccat gaatctcctg gaagtccaca acctgatggt cttctacgag aattccgtcg
    60661 gggtcaacaa cgtcggcatc cagtgccggg agggtcgaat cacgggcgtg ttcgggtcca
    60721 acgccgcagg caagaccagc ctgatgtacg caatctcggg aatcatccgg gatacggccc
    60781 gcaaggaaat gatgcggggc ggcatgcgca tcgccgtgct gggggacgtt ttctttgggg
    60841 gggaaaacgt gacggacctg gaggcgagcc gaagggcgcg caagggcctg gtcctgtgcc
    60901 cggaacgcag gaggatcttc aaggaaagca cggtgatcga aaacctcaga atggggggct
    60961 tcctcgccgg caggacggaa gctcggcgga ccctcgagta cgtgttccac ctgttccccg
    61021 acctggcgag gctcaaaaaa cgggaggcgg ggtttctcag cggaggggaa cagcagatgc
    61081 tggccatcgg ccgggcgctc atggcccagc cccggttgct cttgctggat gagcctctcc
    61141 tgggtctgag ccccgccatg gaagacgccc tcatctcggc cgtggccgac atcaatcggg
    61201 agacgggcct gagcaccctg atcgccgaac aatacgcacg cccgatcttt cccattctct
    61261 cctacgggta cgtcctcgaa aacggaggtg tcacggcgga gggcacttcg gagtcactca
    61321 tggaaaacga ggatgtccgg gcggcttatt tcggcatgac ggacgaggag gacgaggggt
    61381 catggcccag ctaagccaac gggtgatcga cggggtctac cgcgcccggg aacgattccc
    61441 tcaccacgcg gccctggtgt acctgggcac ttcgttctcg tacgaggccc tggtgaggct
    61501 catcgaacgg ttcgctgcgg cgctgcacca cctgggcgtc cgcaaggacc acaaggtgat
    61561 gctctatttg cccaacactc cccagttcgt cgtcgcctat ttgggggccc aactcgtggg
    61621 gggagtgccc gtaaccgtgt cccccattca cactccctcg gagctgcgct acatgctcga
    61681 cgattcgggt gccgagacca tcgtctgcat ggacgtcaat tatcggtacg tgagggaggc
    61741 cttccccggg agccctttgc gaaggatcgt cgtcacaacc tacgtggacc tgctgccccg
    61801 gtacaagaag gtgttgggtt tcctcttgga caaagtcccc cgggggagat tcgaaaggag
    61861 ggatgacgtc caccgattcg caactctttt gggacattcc cctccccggc ctccccgggt
    61921 ggacgtggat tccgcccggc agctctgcgg catcgtctat accggcggca ccacgggatt
    61981 tcccaagggc tgtgctttca cccacgcggg catggtctcc tttgtggacg agatccaaca
    62041 ggtcggagag ggccacatcg gccggggtcg agacacgatg gtcctggcga acccgttgtt
    62101 ccaccagttg gcccagggca tgctcctggg aatggttttg aaccggggaa acaccgccgt
    62161 cctgatgccc atccccgaga cggacgccat cctggacgcc gtcgccaggc accgggccgc
    62221 cctgttggtc gcatccccga ccctgtatcg cagaatcctc gagaacgagc gcctcgatca
    62281 gtactctctg gactcattgc ggttctgctg gagcgggggc gagatgcttc cggcagaggt
    62341 tttcaaccgc tggaagaaga ccgtcggccg acctgtttac caggtttacg gctcggccga
    62401 agtgggtttc atcgccatga gtcccctcga tcgcgaaccc tctccgacca gggtgggaag
    62461 acccctgccc tcccggaaga cgcgggtggc ggacccggag accctccgac cggtcccccg
    62521 gggggagccg ggagagctgt gggtgacctc ggaattcatc tccaaggaat actggaacaa
    62581 gcccgatgaa acccgaaagg cctacgtcga ggtggacggg gagatctggt accgaaccaa
    62641 cgacatcgtc ttcatcgatg acaacgggga actcgggtac gtggaccgtc gggcggatat
    62701 cattcaacac cggggctttt gggtctcctg ttccgaaatc gaggccgtcc tccaggctca
    62761 cccctcggtc gtgggttcct gcgtggtggg ggtccccgat gcgaagctgg gggaacggat
    62821 caaggccatt gtggttctaa aggaggacgc gcggggcgtt gggggacccg aactcatcgg
    62881 gtggtgccgg gatcgcctgg accctcacaa ggtcccccag tatgttgaat tccgggacat
    62941 gctcccccga tcccgggttg gaaaactcct gagacgcgaa atccgggacg aggaacgaag
    63001 aaaggtcgtt ccaaagaaaa ccgagccctc gtgaaccgtt tcgcggtctt cgtgtgcttc
    63061 gtggtcttcg gagggagaaa ccctgggcgg gcgcccgccg aagagaccga aaacccgggg
    63121 aagggttctc cttgcgggct ttccacggcg tgagcacagt cttccgtggt ttcgcggatc
    63181 ttttctcgag aagctctccc agtccggcga ctgctcatgc acgttccaca ttggccttga
    63241 tgattcggcc gcctcttgct accaatggaa cccgtcccgt cgatcttttc acagtcactc
    63301 accctcccgc gggagaaaag cctgtgaccc ccgagaaatc ccatgatttc ttccaggttc
    63361 tgcagcatca cgggcccgat cgaggagaaa agacggctgt cctgtgcgag ggagcggagg
    63421 tgtcttacgc cctcctgaac gaccatatcg gccggttcgg caatcttctg aaagaccggg
    63481 gcgtccgacc cggggaacga gtgctcatcg ctttgccgga ttgccccgac gctttctatg
    63541 ccttcctggg aagcatgcaa tgcggcgccc tgcccgttct cgtgaacccc gatctgcccc
    63601 gaggcgactt tgaattcatc cgcgaagact cccgggcttc ggtcctggtc accgtcgggg
    63661 acggcccggc ggcagaggcc tggacctccc ccctcgaacg gcgcatcctc gtcgacgacc
    63721 cgggataccc gaccctcttc gccaatgcgt cgccggtgct tgaggtcgtc ccccattccc
    63781 cggacgaagc ggcgttcctg ttctacacca ccggaaggaa cggcgatctc cgaggagtgc
    63841 cccatcgccg gggcgacatg gcattcagcg ccgaaaatta tgccaggggc gttttgaacc
    63901 tgtgccaaag cgatgtggtc ttttcgacga gcaagctgtt cctcagctac ggcatgggaa
    63961 acagcctggt ctttccgctc tacttcggcg ccacggcggt ccttctgccc tcacggccca
    64021 taacgccgga tcatgttttc gccgtcatcg cccggtcgag acccaccgtc ctctttgccg
    64081 tgccgacact ctacggcatg atgctgaaaa ccctggacga aggggttcgt ctcgattccc
    64141 ttcgcctgtg cgtttcagcc ggagaatccc tgcccgcggg gctgcaccgt gactggaaag
    64201 aggcgaccgg gctggaaatc cttgagggca tcggttccat ggaaaccctg cacatcttca
    64261 tctccaatcg ccccggccat agccggcccg gcacttccgg cgtcccggtg ccttcctacc
    64321 gggtgcgcat tgtcggggaa gacggcttgc cggtcccttc cgggcagccc ggctctctgc
    64381 aggtgaaagg aggaagcatc gctccctttt actggaacag gccggagttg gccgcagaga
    64441 ccatgctcga gggcggctgg ctcaagaccg gggacatctt caccgaagag gacggtttct
    64501 atacctatcg gggacgctcc gacgacctct tcaaatccgg gggaaactgg gtttcccccg
    64561 ttaaggtggc gagcattctc cgggagcatc cccgggtccg ggattgccgg gtaacttccc
    64621 ggaaactgga aggtctgttg aaacccatcg cccatgtggt actgaatccc ggcccggaag
    64681 gcaccccgga g
//
LOCUS       DAOOVC010000005        64417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_249_length_64417_cov_6.870793, whole genome shotgun sequence.
ACCESSION   DAOOVC010000005 DAOOVC010000000
VERSION     DAOOVC010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 64417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64417
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_249_length_64417_cov_6.870793"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2488)
                     /locus_tag="PLM79_01250"
     CDS             complement(<1..2488)
                     /locus_tag="PLM79_01250"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HOV84960.1"
                     /translation="MEEKLADLEEYRRIKHAIAEEIRSLGEFHREGGSRALAMECREL
                     MERLAEDRFNLAVVGQFKRGKSSIMNALIGRDLLPTGVLPLTSVIVNVRHGPQERLVI
                     RRGNGLYPESFPISNLFEYATEKGNPGNTKGVVRAHVETPAEFLRPGLDFVDTPGIGS
                     ATDANTEAARRFLPHCDAAIFVTAVDTPLTRMELDFLGTVLGYVGKIFFVVNKTDLVN
                     KAELDEILQYIREVLSREMQLGPIRLFPVSSKLAMEASIRQDTAGYALSGMADLKSHL
                     ARFLAYERKSTFLAAMADRALRLASGAETAARLAEFASRGSVEGIAAIRRELEARLGQ
                     LREERTDLETVSRRQLTAWLTERLVAPLQGFFQSKEDELFREMKQLLERHPRQGRRKV
                     LERVVRYSKLRLACDLDGWMAESLEHLKPDFSAKLQQIASTLELHLGRIPRVAAEVLG
                     LDRVEMPEVGPTFRDPHLGNLAPLGPLRFDWPRHLPVPLGFLPVVAAGRSTQETGKAQ
                     IRQLFAVCSDTVTREATRYVGEVLAPLFSRAEDCATRAGEAILQVLDKGGPSVFDRRG
                     TGCPLPTARLAEIEERLCTIREAIHTGPDLSREIPTPSSAHLKEPGPVAPEPPGIDTD
                     IEKDLGTRGCPVCARLGKSALAFLAKWQYALAHEKDTQLAFARETGFCPLHSWHLASV
                     SSPLGLCLGYPRLLERLEGELSRLSSSAFGADELSSLVKDSRDCRVCRLLQDVERTYL
                     ADLAHFLEEPRGQQVYAQSHGLCLRHLAALTRILPSKPLRQFLAAQAARKYARLAEDM
                     RNFAMKRDALRKDLLNRDERDAHHRALIHIA"
     gene            complement(2572..3468)
                     /locus_tag="PLM79_01255"
     CDS             complement(2572..3468)
                     /locus_tag="PLM79_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV84961.1"
                     /translation="MGPFAVTRKQEPDLGPTGRTRPNSGADRAQPLLEVHNLWCGYGR
                     HTALKGISFRVAPGEIIGVLGPNGAGKSTLLLAAGGILPLRSGEIRLLGSPLSQWKPK
                     ERARRMAALNQDCDVRLPFPCGEVVLMGRYPHQEFFRTNGIRDATAVQWALDRTDTTE
                     LADRLITEVSGGERQSVIMARALAQQAPLLLLDEATSAMDIHRKLKIFQGLERLNREE
                     NLTILAALHDVNLAALFCRRLIMLKNGEILADGETRSVLRPEILENVYGTPAVIQEIK
                     EIERLQVFFLPSGETSKGNGDG"
     gene            complement(3545..4816)
                     /gene="eno"
                     /locus_tag="PLM79_01260"
     CDS             complement(3545..4816)
                     /gene="eno"
                     /locus_tag="PLM79_01260"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOV84962.1"
                     /translation="MKERIADIRALEILDSRGNPTVRVILRLDNGMKAVASVPSGAST
                     GEHEAVELRDGNPARYGGKGVLKAVENVNQVIAPLIVGMEPIRQVEIDRIMMEADGTP
                     NKGKLGANAILGVSMAVARAAAEACGLPLYQYLQGPGPARLPVPMMNILNGGKHANNS
                     VDFQEFMVMPLGAPSFAEALRYGAETFHALGRILKQKGYATGVGDEGGYAPDLRSNEE
                     ACELILEAVAAAGYEPGKDIAIALDPAASSFFENGFYELNKSGLGRMTSSQLTDLYRT
                     WVDKYPIVSIEDGLDENDWSGFQEHTAALGTRIQIVGDDIYVTNSRFIERGIREKTTN
                     AVLIKLNQIGTVTETIEAVHLCRKAGWGFVISHRSGETEDTFLSDFAVAMGGAQLKTG
                     SACRSERIAKYNRLLEIEAELGRAAVFRSPL"
     regulatory      complement(5527..5590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(5739..6161)
                     /locus_tag="PLM79_01265"
     CDS             complement(5739..6161)
                     /locus_tag="PLM79_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV84963.1"
                     /translation="MNIQRILIAYDGSPESERAYEMALDLASRYTAELVVVSVARPPE
                     PPIAVEMEAVLEHATEYYRSRFASLKDQAFVQGVEPRFEVRIGHPAEQIVILANEEEA
                     GMIVMGHRGGGSFLQQWRLGSIARRVMNYAQCTVVVVR"
     gene            complement(6164..7381)
                     /locus_tag="PLM79_01270"
     CDS             complement(6164..7381)
                     /locus_tag="PLM79_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOV84964.1"
                     /translation="MGETWFLAALWLGLALFAALLSAWFRIATALSEILVGTLAQLVL
                     GGMAGAAVLGLDEPWIKFLSGTGAIMLTFLAGAELDPEVFKLKWKEASAVGLASFLAP
                     FVGCAAMAYYFLHWDARASWLAGVALSTTSVAVVYAVMIEFRLNTTTFGKTVLAACFI
                     TDLGTVLALGSLFAPFSPKTLFFVLATLVGMVLLPWATPRFFGIYGGRPSEIEAKYLL
                     LVLFGMGGLALWAGSEAVLPAYVVGMVLAGTVGRDHVLIRRLRTLTLGLLTPFYFLRA
                     GAFVSVPALLGAPLIFLLLLLGKVAAKFVAVYPVTRMFRSSGQEGMYTTLLMSTGLTF
                     GSISALFGLTHGIIDRPQYSLLVATVIASAVVPTMIANAYYLPRHLLQPGEKRGTPAV
                     EGARSGELATQGD"
     regulatory      complement(7751..7813)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     regulatory      7953..8016
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            8071..9837
                     /locus_tag="PLM79_01275"
     CDS             8071..9837
                     /locus_tag="PLM79_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOV84965.1"
                     /translation="MLDYPKIRDGLLQVVDRLSDTVRSETSNSLNLVRSKLEEEAFNL
                     VILGQFKRGKSTFINALLGESILPTAIVPLTSVVTILRYGPTLRVEVRYLDDRREEIR
                     LSDLPAYITERENPENQKGVKEVTVFYPSEYLKGGVRIIDTPGAGSVYSHNTDVAYGF
                     LPQVDAGIFVISADPPMSKSEHQFLKDVRQYVDKLFFVLNKIDQVSLEDREESLSFTM
                     QVLEDDLGTGKVKIFPLSARWALEGKKAQDREMVSRSLLPDFEQRLREFLLHDKGRVF
                     LSSIINSLRKLISDETISFRLEQEAIKLPLQVLEEKIARFEAEMALIQKDREKNQYLL
                     AGHLKKMTGQLDEEIGEFKRKQLPALLKELEEEYLRRTGRGGGNLREELEQFVFDRIR
                     QSFGTWRIQWAEKISVRLEEAHREFAERINETIDRILALTGNLFELDLKPFSAVESLS
                     TKSDFYFLLKDDPVGLELIQLAVTSALPQFLAKKMILKTMRTQVEELVDRHCGRVRYD
                     MVNRLERTVRDFQHALDEKIDLTLEGIRISFRKALALHSKGAEDMTRNLGELSARLGS
                     ISRIHEELLAHATVVSDGVGEN"
     gene            complement(9927..10817)
                     /locus_tag="PLM79_01280"
     CDS             complement(9927..10817)
                     /locus_tag="PLM79_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOV84966.1"
                     /translation="MEPKVPIMLITGTLGSGKTTLLKRILQNARQRIAVIMNEFGEVS
                     IDSKILEGDHLKIIDVSGGCVCCSMTGEFEAAIRELVETIRPEFIVLESSGVAEADAL
                     VLRVGGGMPQVRIDSVVYVVDAYAVAMYPYIGMSARSHIESADIILINKTDLISLQEA
                     EQVETAVREYNPRAIFFRTAGCEVDTNLLFGLEVERRPRMGGPFRDMTTFESVSFATG
                     KPLGEQRFRELVRRLPSQVYRAKGFVRLDGRGFLFNYVAGRSDLEEFSSDQTRLVFIG
                     RGVKAVGGEILQGLRACEVS"
     gene            11269..14502
                     /locus_tag="PLM79_01285"
     CDS             11269..14502
                     /locus_tag="PLM79_01285"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOV84967.1"
                     /translation="MIGRPGRWTALTVCLVFTFCIGIGGLGASPPRDKDPVPVPPVSD
                     TLGKLPGKAWKGPGASRIQKEIDQPNPKDYRKMQLRRQLQEALDEAARSEEDRPEVRA
                     QAERLAKTGKDKVLVILVEFGGTDVFTWTQGVSTWDPLGKADPNESTGIAGDCSKIIT
                     STAVFSYTGPLHNQIQRPLSESDRSGDMIWTEDFDSSYYQGIIDGNGVVFDYVRQDNS
                     TVHEDFTGKSVRTFLEDQSGGLYTIEADIYGWLQVPHSIWWYGADPCPGARSGLDVGH
                     NGAIPGAGNAKALVRDALDQLKALHPEPSFWAQYDQDGDGYIDRLWIIHAGLGEEDST
                     TLLNRTPYGEGGLWSHSSSLGSYEIVQGIYAGPYIMMPENCGIGVLAHEYGHNLGATD
                     LYAYGFGETSAGFWTLMADDWTGYPIGFLPPNLDPYHLDALGWLNPLTVTDPTKIYKV
                     TVGQAGNFPGGAGVYRGVKIPLKKGVLPLPVQPEGSYQWWSGQGDLVNATMTLTDTIA
                     IPGGGYRLKFDLAYDIEPGWDFLWVQVSGDGGNNWTTLTNGNTTCAHDADWIGGAYGF
                     PDDLCAAGIGGFTGNNGSYPAYEEQSFSLGNWAGQDILVRFWYMTDWNTMGNGAFIDN
                     IAITSDEGVPIFQDDGEGGGGNWNYAEGFELNNGTRTFTHNFYFQWRNVNDPDSFDSA
                     LGDSRWRFGPANTGLLAWYNNNFYGDNEVWDHLFDDFGFGPKGLMLVADAHPEPYRDP
                     YYVGVGYNNEGGNVDHRSLMRDAPFSLWDSVDFTMQPPYVYQEASFSGRPAVSVFSDD
                     VGYYPGSEYVSRGPGYDPSVAMKWVTHQWDAGVVMPSRVFYGIRAPGYTGDQEFRFNC
                     SRLAGGRLGCYWLGAGVGLGYDGSNGNPGEVSGQYGWKAQILSQTEYTATLKIWNSVV
                     PVADFSADVVKGKAPLDVKFTDKSRGEIQTYEWDFGDGNTSTESDPTHTYSKGGKYTV
                     KLTVKGPHGTDTKTRTGYIQVYVPPTANFVAAATKGKAPFRAKFEDKSKGVVTKWLWK
                     FGDGATSTKENPAHVYKNPGRYTVKLTVKGPGGSNTKTRSNYIVVVK"
     gene            14841..15650
                     /locus_tag="PLM79_01290"
     CDS             14841..15650
                     /locus_tag="PLM79_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOV84968.1"
                     /translation="MVKLSALGLVVLVLAGCARGLSCSRPDGRFMFARTRVQPAERLV
                     SAAPSLSQDVSVDLAETTQSLAEQESALAQKEGEARTAALCELARLCFLLGEYGEKSE
                     REAHFSKGRTFAEILRQEEPRRVEGYYWLALNLAGLAEAGSAARALGMVPTIIENLKI
                     AVEIDETYDQAGPHRVLGRIYCEAPCWPLSDGDVKKSLHHLRRAVEIAPDNSTNHLYL
                     AQTFIQLCELKEAHRELQQVLSGTQHATWPAGLQEDQLEALDLLKRCGKPD"
     gene            complement(15728..16921)
                     /locus_tag="PLM79_01295"
     CDS             complement(15728..16921)
                     /locus_tag="PLM79_01295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HOV84969.1"
                     /translation="MDPKHPDPHLQQRTITVLLIDDQPIVAEAVRRMLKGEEDIELHY
                     CPDAARAVQAAVTLRPTVILQDLVMPEMDGLTLVRYFMANAKTRDIPLIVLSSKEEPI
                     VKARAFGLGASDYLVKLPDKIELIARIRHHSRGYISFLQRNEVMTRLAAELNEAADYV
                     RKLLPAPISRGPILTDWRFLPSASLGGDTFDYYWLDDDHFAMYLLDVCGHGVGAALLS
                     VSVMNVLRTQTLADADFRRPAQVLSALNERFSMENHKDMFFTMWYGVYSPSSRELVFA
                     GGGHPPALLISPSAGGDGTQTTLLTNTNTMVGCIPDYPFQSASAEVPASSRLYLYSDG
                     VYEIQCADGSMWDLEGFVEFMEGACTPGGQGEACLDRLWQHVRQMRGKESLEDDFSIV
                     QFAWP"
     gene            complement(16965..18038)
                     /locus_tag="PLM79_01300"
     CDS             complement(16965..18038)
                     /locus_tag="PLM79_01300"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOV84970.1"
                     /translation="MRIAIVNDLLMAVEILRRVVLSGPGYELAWIARDGSEAVARCSR
                     DLPDLILMDLIMPEMDGVEATRRIMRDTPCAILIVTATVMGNADKVFDAMGAGALDVI
                     ATPVLGPQGKIRGGKELLAKIARIARLIQKPSPAPPVETGKDPAVPTGLLPPLVVIGA
                     SAGGPRAVAGLLEALPENFGPAIVVIQHVDADFGMKLVDWLRRQTSVQVRSAEEGTKP
                     AAGVVLVAVNEHHLVLGPDLRLSYSPEPLDAVYRPSIDVFFDSAAAHWKAKGLAILLT
                     GMGRDGARGLLHLRRAGWHTIAQDEKSSTVFGMPKAAIELGAAAEVLPLSKIPAAILK
                     MNPLRLKDPPSRSTTKFSPRKPL"
     gene            complement(18063..20405)
                     /locus_tag="PLM79_01305"
     CDS             complement(18063..20405)
                     /locus_tag="PLM79_01305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOV84971.1"
                     /translation="MAGGDLGILADEGMLELFRMETEMHAGTLEKGLVDLEGNPSDTG
                     VLESLMRAAHSIKGAARILGLDMAVRLAHAMEDLFLAFQARQRILNPEMGDLLFKGVD
                     VFKRLSRVQPPEILSWIRENEPAVLEMEEGIRALLSGPPPDSDLPPGEENSPGPLLFT
                     APDPTPATVSAPSPEPEPARIEAQTGPPAAGGSVPAHGGPEDAVPVSAESLSRMIGLT
                     GECLIESQVLRSRMQELQQLKSLLIRLTKTAERRRPSSLEGDSEARDRQVLAEIIKMA
                     GECRDNLSRQIEFFDTYLLRWENLSGRLYHEALAMRMRPFRDGIHGFPRMVRDLARSL
                     GKKIRLRTSGERTKVDRDVLMRLEAPLTHILRNACDHGIEQPAERRAAGKPEEGTIEL
                     KAAHRAGMLVVVVNDDGRGIDPERIRSVVAAKGMVPPEVVRSLSDAELMEFLFLPGFT
                     TTDGITELSGRGVGLDVVKKMIHEVQGTIRVQSAPGVGTSFQLELPLTLSVIRALLVE
                     ISRELYAFPLTRIDAVRMVPQEEIRLIEDQQYFSLEGRNVGIVAASQPLGLIPIPVTD
                     SELSVVLIGDRLNSYGIVVDRLLGEVDLVVRPLDPRLGKVPNLSAAAVSEDGAPVLIL
                     DVDDLVRSVDGLLTGGRIVKITRSEVAAPQTAVKRILVVDDSITVREVERRLLAARGY
                     QVDVAVDGMDGWNAVRTGKYDLIVSDVDMPRLNGFELVRKIKSDPQLSRLPVIILSYK
                     DREGDRVAGLEAGANYYLTKSSFYDETYVNAVADLIGLPG"
     gene            complement(20387..21136)
                     /locus_tag="PLM79_01310"
     CDS             complement(20387..21136)
                     /locus_tag="PLM79_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOV84972.1"
                     /translation="MTDRFREPSSKDLALPAGRQEEGTENIPPDCWRVVGIAGDGTCG
                     ELEGFIHCRNCPVYSRLGRSLLDRPPPPGYRTEWTSLLAKASREETSREVSVVVFRIG
                     GEWFALRSRCFEEIVTLRSIHTIPHCSNSVLLGLVNVRGELLLCMSLHQLLGLEEASR
                     PQTRQEPLETRRMAVVRDGDSRWVLELDEVDRTYRFPLSELEPVPETVARSHESYSCG
                     IRDLAGKRVALLDERLLFNALNRSTRWRAAT"
     gene            complement(21133..22515)
                     /locus_tag="PLM79_01315"
     CDS             complement(21133..22515)
                     /locus_tag="PLM79_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOV84973.1"
                     /translation="MRPQAIDRFLCDQMGYDPRCVPFRALQRHVRERMKICGIPDEEA
                     YLALVTENEEERDELLEALLVPETWFFRDFEPFVFLREYIRRKRFPAPGGGRPWRVLS
                     LPCSTGEEPYSVAMTLMEAGLEGSQFHIDAVEISRKALEKAERGIFGPSCFREQDEDL
                     RRKYFEKSGRSYRLPARVRGAVRFIRGNLLDPGGLAVNGPYDIVFFRNLLIYFSDQAR
                     ARATETIDGLLACGGLLFLGYAEPSHIFFPGYIPVDHPRSYACLKPESAPVEAHRPLS
                     PRCGTGEQPGNEGRKGFPSRVPGDRPGAEGPCASTRSPRSSADDRIQGTGTPMGAPGG
                     RESLGLPSLIEATGSEDRKRRTEALLESARGLADAGMLREAGERCLECLELDVTCCEA
                     HFILGIVQWAMGDAEGADRSLQKALYLDPDHLDALFHLSLLLEERGLAEGADRCRERM
                     ERIKRREMSG"
     gene            complement(22518..22982)
                     /locus_tag="PLM79_01320"
     CDS             complement(22518..22982)
                     /locus_tag="PLM79_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOV84974.1"
                     /translation="MLLVVFSIGGDRFGLDSREVVEILPLVEFRKLPRAPEYVCGLFQ
                     YRGKIVPAIDLARLAGTRESHGFLSTRILLVRYPDEAGENRFLGLVAEQVTETVKVDE
                     RGFSSPGIHVKDAPYLGEITRDSRGIVQRIEIGQLLSPDVRELLFQPAEASA"
     gene            complement(22985..24973)
                     /locus_tag="PLM79_01325"
     CDS             complement(22985..24973)
                     /locus_tag="PLM79_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV84975.1"
                     /translation="MKISSKLILLVSLGIMLIGVQFGINYFLNRVMRNFDEGIRQLNA
                     VSENLLNGIIEERNFIHSRKDTSFEAALASLKRAEEGLSALEGGTSVETGDVALFKGL
                     LEKYREAFDHLGQAVKKLSAFEVEFHETLLGFNKKTEAVVQKLSKEADAKAAQGQQAA
                     ASLKSLLDDARQISFLTNQMFLIYYVELFQKSDVSSYLARSQKVVMELERERKSVTAI
                     PLVYVDRDYSDFVTEALTKTIDSLPGQVNKLWELSTDRIMAQTDLDQIRAKVTGAKEQ
                     IIATNNARIVRFGNMLLGANVVAFLVAASVVLFVGVMIVRSITGPIQRITGSAEKIAQ
                     GDLQGAADDLSSFGTETDASGQLRVKKDEIAHLSSVFGHMTRSLHSLIVQVRNSGIQV
                     VSSATEISSSARELEATAVQQAASINQVSATSKEISAGSQELARTMNEVGTVASETAT
                     LAEEGHGGLQGMESTMRYLLDATTSIAGKLEAISDKTSNIGSIVTTITKVADQTNLLS
                     LNAAIEAEKAGEYGLGFSVVAREIRRLADQAAVATLDIEQMVKDMQSAVSSGVMEADK
                     FIQQVHQGVRDVQRISGQLATVIRQVQALIPRIGAANQSMAAHSTGAEEISMAMVRLS
                     EGADQTRQVVREFNRAAEQLNEAVRGLQDEVSRFKVAA"
     gene            complement(25518..27845)
                     /locus_tag="PLM79_01330"
     CDS             complement(25518..27845)
                     /locus_tag="PLM79_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV84976.1"
                     /translation="MDEQQYYRISGLENGRRIESRILEERIQQAVADGVRNIEVEAYG
                     HHGIGGRLWKAGDDPIRVRVVGSSGQRTGSMGFPNTRVEIMGPTSDDVGWLNAGAEIT
                     VHGNAANGAANAMAQGKVRVAGNIGARGMTMTKHNPRFDPPELWVLGSVGDYFAEFMA
                     GGIAVVCGYRPQDPDNVLGYRPCVGMVGGRIYFRGPHKGYSQADALLSPISDADWDWL
                     YRNLEIFLDRIRHGDLLQELAVREQWQLLVARGPHDKYAKDRRSMSEFRRESWDRELG
                     PGGLIGDLVTYDRSPIPLITRGNLRRYVPVWENRKYAAPCEASCPTGIPVHERWRLVR
                     EGRVDEAVNLALAYTPFPATVCGYLCPNLCMQGCTRGAMNLAPVDISQLGRASIKAQL
                     PTLPPLSGKRIAVLGGGPAGISVAWQLRLHGHEAVVYDQRKSLGGKIAFQIPESRIPP
                     EVVEAELDRVRQLIPHVHLQQRLTREDLEAIRADYDFVVVAVGANKPRVLPIPGKERL
                     IPALTFLEQSKTDRVRVGDRVVVIGAGNVGCDAATEAHRLGARDITLIDIQEPLSFGK
                     ERKAAEEAGARFRWPCFTRAVTEEGVELTSGEVIPADTVIVSVGDMPDLDFLPESVAV
                     ERGFIRVNELNQTTDSRIFAVGDAVKLGLLTDAIGAGRRVARVISDLLSGKQPSVRTP
                     GKIDLSRVKLEYYDPRVLHMEGVQQCASQCSSCGNCRDCGICTAICPESAISRWEGDG
                     GFEMRVDPDRCIGCGFCAGACPCGIWNLVENDPLE"
     gene            complement(27866..29524)
                     /locus_tag="PLM79_01335"
     CDS             complement(27866..29524)
                     /locus_tag="PLM79_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOV84977.1"
                     /translation="MLPNSPITPSTLSVKDLPWQIRWERDRCTLCGRCTAVCPVHAIE
                     LGVYRKRDIRLGSLDGGETQGPPSNVYSVFHGIRQRTDPAYACVGCASCNLVCPNDCI
                     IPYRSDEADKLRFHINRGGQPRSRGGRRNVPDSPPGGLLDRIKFIRISMLTDPALDAG
                     RHEFELRTLLGRVLPPEEGLKVYREQGWIPPVREIYPLIIGAMSFGALSPNMWEGLQM
                     GVAYLNEELGMPVRMSTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEIIHALPEMKE
                     DPCAVEIKYGQGAKPGDGGLLMWYKVNKLIAAIRGVPHGVSLPSPPTHQTKYSIEEAV
                     AKMIQSMYMAWGFRVPVYPKISGTSTALAVLNNLTRNPYAAGLAIDGEDGGTGAAYNV
                     SMNHMGHPIAANLRDCYLNLVRVGKQNEIPLFAGGGIGKYGSLAANAAALIMLGASGV
                     QTGKYMMQAAAGCLGSESDRCNICNIGLCPKGITSQDPRLYRRLDPEKVAERVVDVFL
                     SFDTELKKIVAPLGRSTSLPIGMSDALGINDADAAQRLQIQYVV"
     gene            complement(29488..30618)
                     /locus_tag="PLM79_01340"
     CDS             complement(29488..30618)
                     /locus_tag="PLM79_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HOV84978.1"
                     /translation="MCRLFAMTSETPLSPMVAVQALDVMREGHDGSGVGLFLTDLGGH
                     FEELKGAPILSGIFTNEGLKRLDQFMMNMGFMTKYKISIKVPKTPPPGVPKRDIYLIR
                     AYEYPESWETLSEQEIRHRLMLVRLELREMGREKEDMIVYSFWPDVIMIKEIGDPLRV
                     ADYLSLGRKDILARVILAQGRQNTNYAINLYACHPFFIQGVATMTNGENTAFVPIKEF
                     LMSRGFPGYIGYNSDSEVFTHILHYSLNRLGLGIEAYKHLITPLQDADLAVHPDGGFL
                     KQMKNSCRRLIIDGPNCVIGCLPDKTLFMVQDRKKLRPGVVGGKPGVYAFSSEMCGLD
                     AVIPDRDETQDFQPMHLDTVFVRPDRQEVHICSQTAPLPPLH"
     gene            30813..31598
                     /locus_tag="PLM79_01345"
     CDS             30813..31598
                     /locus_tag="PLM79_01345"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOV84979.1"
                     /translation="MLWLRKRRRNRIRSRPFPAAWVHFLEKNVPYYRLLPEEDKKELQ
                     GHVLVFLEEKHFEGCGGLELTDEIRVTVAAQACILLLHREAGYYPRLDSILLYPGAYV
                     ARGHEELWPGQLVETDQVRLGESWKAGLLVLSWDHVRRSASSPMESHNIVLHEFAHQL
                     DLEDGLPNGAPLLPDRSSVRTWARVLGEDYRRLRRDVWRGRETLLDQYGATDPAEFFA
                     VVTECFFQKPLEMKAHHPRLYEVLKSFYRQDPAELVERQEALS"
     gene            complement(31591..32481)
                     /locus_tag="PLM79_01350"
     CDS             complement(31591..32481)
                     /locus_tag="PLM79_01350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV84980.1"
                     /translation="MTASFKDALHSGDFLVTAEIGVPKGIDIEDTKHYIGYLKDKVHG
                     LKMTDEPDNGVHLPSLVVCHLIKEMGGVPILQVSAGDGNTSFLQSNLLSAAVLGIDTV
                     LSAAVTSLRSGGHRPSFGPEDPDSLQILRAIRTLESGRDLGGHALKKAPKFCAGAVLV
                     LGTRSLEDQLRDLEARIRAGAEFVLTQPIYDMDSFRRFMDRARNYRVKILAGIAPLLS
                     AGVARFMNRNVPGMAVPGDLISELEQVPKKDQAKKGIEIAARMVRTLKEEGICHGVHL
                     VAAGREKAVPEILHRASLLN"
     gene            33329..33547
                     /locus_tag="PLM79_01355"
     CDS             33329..33547
                     /locus_tag="PLM79_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84981.1"
                     /translation="MIDVSIKYNVTGGSQPCALCGALTESKHGPDLFLEGTMQVICPE
                     CGQDHAPHLARLLELGQQAQCYTQACAR"
     gene            33900..35198
                     /locus_tag="PLM79_01360"
     CDS             33900..35198
                     /locus_tag="PLM79_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV84982.1"
                     /translation="MSDRPRVVSGVHQLDALLGGLFIGDNVIWHDDSGSLASVFCLNF
                     IRASLDQNRPLIYVSFDRSPRNLLEKLGPLADHPSLTILDCFTSGKGASSPVFLRFYD
                     EPGTDRACEIVRVEEPRKIDHVMEVLYGVHAALEGYVCFVFESITGMQEVWGGEDHIL
                     NFYSHSCPRLYELNTVAYWIMEKKAHSPRLRAQISQIAQVVIDLGIKRGTTSLTILKA
                     EDRNPAVFHKPHNYWTRDLTIAFDEERRIPGGVDLGVRLKELRSKRGLSQTDLARLVG
                     VTPSTISQVESNLIYPSLPALLKMAEVLAVDVSSFFQEKPSAGHRLVFTAADAVEVKL
                     PQIPEGFAYARLLTPPDLEPRAEPFLIEVPPNTTIPSHFFFHKGHELGYLVSGKLQVK
                     FGQASYTLRGGDVLYLDSQLPDQWKNPGSSPARLLLIKIR"
     gene            complement(35344..36936)
                     /locus_tag="PLM79_01365"
     CDS             complement(35344..36936)
                     /locus_tag="PLM79_01365"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOV84983.1"
                     /translation="MNLSTPNSNDATLTFNRSKSVVPMSGLCSRCIDGCRGNCEVFRA
                     TFRGREVIYPGPFGQITAGADKNYPVDYSHLNIQGYALGGAGLPGGLEANSDTAVFPA
                     VSTETEYGWDIKVKMRMPVFTGALGSTEIARKNWEHFAVGAAISGVTLVCGENVCGID
                     PGLELNADHKITKSPEMDRRIEIYKRYHRGYGELLVQMNVEDTRLGVAEYVVDKHGLQ
                     TIELKWGQGAKCIGGEIKVHSLERALELQKRGYIITPDPSSPVAQAAYKEGAIKEFER
                     HSRLGFVSEEGFLAEVARLRKLGFKRITLKTGAYSLRELAMAIKWGSKAQIDLLTIDG
                     APGGTGMSPWRMMEEWGIPSLYIHSAAYDFCQKLTARGERVPDIAFAGGFSSEDGVFK
                     ALALGSPFVKAICMGRALMIPGMVGKNIAEWIQKNDLPKTVSQYGNTAEEIFVSYEAV
                     KDLVGDAEIKNIPLGAIGIYSYSDKIRVGLQQLMAGSRCFNLSSISRRELMSLTEECT
                     RVTGIPYVMDAYREEAEAILNS"
     gene            complement(37436..40171)
                     /locus_tag="PLM79_01370"
     CDS             complement(37436..40171)
                     /locus_tag="PLM79_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84984.1"
                     /translation="MPDLSRIPLCSQYLGTLLRARPELRQWLSSPGNLHRRYGLTDLF
                     QDLERTARSAPSFEGLLRTFREFKQRHFLRIGARDLLGMANLAETTGQLSDLATVCLQ
                     VGLDVLDQSPQWWADKEESRAWATWRGRLGLVVLGLGKLGGHELNYVSDVDLLYLYSH
                     EGMEEALPDSIPVLVQRLFQWLGKLLSDHVDGDRVFETDLRLRPGGKDGPLVPSLSGA
                     ADHYLQHGRPWERQMLLKARPVAGNRSLGTAFLQEVRPFVFRRFLDFQALDEIRAMRD
                     RILSEAPRPRSDWENFDVKLGVGGIREVEFLVQSLQLIYGGRHPELEEPNSLRSLQAL
                     RDLGLLSDSVAESLAQSYSFLRRIEHFIQLDQNRHSSRIPRSPESLLRICRAAGIGEN
                     VRDLFDRLQEHCRRVHEHFLDLFRSRDPHPRESITDGGEGSRDFPAAYPREARDRLEA
                     HLRDFPADLHNALVRFLRPYGALRETEIREKILLRIERYLAHVRRRPGLTKVFHAFAP
                     WMEEFFSGLGRSELLGELLAHNPSLVEGMATTHGAFPPGPVWERNAARVLDRGLDYEQ
                     EMEWIRRLKNERTVQIAREDLAGRLSDLELELELSGLADFVVRHTYGGVCRNLGLPRD
                     LPLAVLGLGKLGSREMSYLSDLDLVFVYRPAPGESDDEIPGTVLRLVQRFMRMLSTPL
                     QEGPGYAVDVRLRPTGNYGPLIVTERSWIEYYSRDADLWEIQALLRLRTIAGNPSVGR
                     DIEGTASRICYGKRDESRVWARLCHLRQRMQEERAGEHPDLLDLKLGNGGLADVEFLV
                     QGLHLARGHEKVFPAGGSVRSCLKSVLTSIGSGESECREVMVAFKALRALEHRLRLYS
                     NAAGSKISPRQLHEMRALGLWPVKDAEESLENWQDLLRMRKTLRKALGAFCPSLV"
     gene            40552..41340
                     /gene="kdsB"
                     /locus_tag="PLM79_01375"
     CDS             40552..41340
                     /gene="kdsB"
                     /locus_tag="PLM79_01375"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOV84985.1"
                     /translation="MKVVGIIPARYASTRFPGKPLADIGGKPMVRHVWERAASSGSLD
                     RVWVATDDRRILETVRDFGGNALLTGDHASGTDRLAEAARILGLEAEDVVVNIQGDEP
                     LVRAEMLDRLVAGLEEDPAFPMATLAHQTDDEREYRDPNVVKVVVDQLGKALYFSRSP
                     VPFRRDEPAGSPVFLKHLGFYAYRQSFLQTFTRLHPGKLERLEKLEQLRAMEHGYLIR
                     VIPSPWDTRGIDTPEDLERLLEELSGNASSASGERPGERRDEMS"
     gene            41585..42772
                     /gene="carA"
                     /locus_tag="PLM79_01380"
     CDS             41585..42772
                     /gene="carA"
                     /locus_tag="PLM79_01380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOV84986.1"
                     /translation="MTVPWKRTRAVVVLEDGTVFRGFAFAGAGRALGEVVFNTGMTGY
                     QEVLTDPSYKGQIVAMTYPLTGTYGINAADMESEAVQVEGFVVKEYQEFASNWRSEKT
                     LARFLEEHGKIGIEGIDTRALTRHIRQAGAMRGILSTDSDDVPELLDQVRAFPGLHGV
                     DLVRLVTCRTPYLWIRGEKIERPSREWWPGHPGHRVAALDCGIKFNILRKLEEAGCQV
                     MVFPADTPAETMLSVKPDAVFLSNGPGDPAVLEYVIRTVRGLLGQRPVFGICLGHQLM
                     GLALGGQTFKLKFGHRGANQPVKDLSTGKVEITSQNHGYCVDLSSMRDLPVRLTHVNL
                     NDGTLEGMEHLEVPAFSVQYHPEASPGPHDASYLFRRFVAMMERNGPKTSSGLAERNC
                     VHA"
     gene            42765..46004
                     /gene="carB"
                     /locus_tag="PLM79_01385"
     CDS             42765..46004
                     /gene="carB"
                     /locus_tag="PLM79_01385"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOV84987.1"
                     /translation="MPKRTDIGKILIIGSGPIVISQACEFDYSGTQACKALREEGYEI
                     VLVNSNPATIMTDPETAHRTYIEPVTPEAVAKIIERERPDALLPTLGGQTALNVAVRT
                     AEMGVLDRYGVEMIGARVDVIRKAEDRELFRDAMKKIGLRVPASGIARTMAEVEAVAR
                     EIGFPIIVRPSFTLGGTGGGVAYNREELLELAQGGLDASFIHTVMLEQSVLGWKEFEL
                     EVMRDRMDNVVIICSIENLDPMGVHTGDSITVAPAQTLTDQEYQRMRDASIAVLREIG
                     VETGGSNVQFAVNPQDGELVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYSLDE
                     LANDITRETRASFEPTIDYCVVKVPRFTFEKFPKTEDFLTTSMKSVGETMAIGRTFKE
                     ALQKAVRSLEIGRFGFPDPPSRPDPDYVEGLKQAMRRPNSQRLFQLVEAMRLGVEIPE
                     IFELTFIDPWFLHHIRQIVDLEQAVRSWDSPASLMEDEDFLRAAKSWGFSDVRLGQLT
                     GNTEETVRQERIRRGIRPVYKLVDTCAAEFEAYTPYYYSTYETENEARPSSGPKVVIL
                     GGGPNRIGQGIEFDYCCVHASFALKEENVESVMVNSNPETVSTDYDTSDKLYFEPLTR
                     EDVLHIIEEENPRGLIVQFGGQTPLNLAVPLEKAGARILGTSPDAIDLAEDRKRFQQM
                     LRKLGLRQPKNATAFTVEEALAAAGDIGYPVVVRPSYVLGGRAMEIVYDDASMEQFMH
                     TAVHVSPGHPILLDQFLEDAVELDVDAISDGEITVVGGIMEHIEQAGIHSGDSACVLP
                     PITISREMQEEIRRQTRLMARELGVVGLMNVQFAIRNNRIFVLEVNPRASRTVPFVSK
                     AVGIPLAKLATKVMLGKSLKELGFVKEIEPRHLSVKEAVFPFSRFPDVDILLGPEMKS
                     TGEVMGIAPSFGMAFAKAQSAAGYDLPREGTVFISVRDADKPKVLDVAREFRRLGFRL
                     TATGGTARFLRDRGLEVKTVCKLREGRPHVVDRIKNGEIQLVVNTSRGKKTSSDSYEI
                     RRTTLLYNIPYATTIAGARAMAAAVQELQRGEWDVRTLQEYHSEIRNHE"
     gene            46107..47579
                     /gene="purF"
                     /locus_tag="PLM79_01390"
     CDS             46107..47579
                     /gene="purF"
                     /locus_tag="PLM79_01390"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOV84988.1"
                     /translation="MTSDFPTRMTRCSTLVPVRYRPGKREECGIFGVFGHPDAAKLTY
                     FGLYALQHRGQESAGIAVSDGCEMSEHKHMGLVSEGFREETLRALKGDLAVGHVRYST
                     TGSSLLANAQPFVVSHGGEYYAVAHNGNLVNAVELRRDLESRGAIFRSTMDTEVVMHL
                     LARNLRLGLEEALVETLRRVQGAYSLVLATRDKLMGIRDPRGFRPLCLGRLNGSHVLA
                     SETCALDLIEATYVRDVEPGEVLIIDKNGMSSLYPSPEVRPAHCIFEFIYFARPDSSI
                     FNQNVYLWRKRLGHLLAREYGSLGGDLVMPFPDSGNYAALGFAEASRVPFEMGVIRNH
                     YVGRTFIQPSQAMRDFGVRVKLNPVRDLLQGKKVVLVEDSIIRGTTTRTRINALRQVG
                     AREVHMMVSCPPHRFPCPYGIDFSTKGELIAASHSVEEIRRFIGLDSLNYLSLEGLLE
                     GGNAVLNDHPFCLACFTGDYAVPFKEQVRKDCFERQEHRS"
     gene            47588..48571
                     /locus_tag="PLM79_01395"
     CDS             47588..48571
                     /locus_tag="PLM79_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOV84989.1"
                     /translation="MDTGEILEIAAEVLKIEAEGILHLVDRLEESFAQAVQWIYGCRG
                     RVIATGIGKSGIVGRKIVATLSSTGTPALFLHPVEAVHGDLGMIRGEDIVLALSNSGE
                     TNELNVILPSLRNIGTRIISFTGKTDSTLARHSDLAIYAGVPREACPMGLAPTASTTA
                     MLALGDALAVALIKLRNFQERDFHRYHPAGHLGERLDVPVCEVMRRGEAIPVVHEDTP
                     VLEALEEMSRKGMGATLVLGKREELLGIYTDGDVRRTLNRYPDVREVPMRRVMSVKPR
                     TLSMKCSVAEALEMMERHLITVLPVVDGEDRLQGILHLHDLLGKGRIRFTR"
     gene            48840..48911
                     /locus_tag="PLM79_01400"
     tRNA            48840..48911
                     /locus_tag="PLM79_01400"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48872..48874,aa:Thr,seq:tgt)
     gene            complement(49053..49289)
                     /locus_tag="PLM79_01405"
                     /pseudo
     CDS             complement(49053..49289)
                     /locus_tag="PLM79_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            49418..50191
                     /locus_tag="PLM79_01410"
     CDS             49418..50191
                     /locus_tag="PLM79_01410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV84990.1"
                     /translation="MDMWKFFDITHRDHIVCNPMSLEKLEQLITLLSLKPGARVLDIA
                     TGKGEFLIRLAERYPQMTGTGVDFSPYCIADVRKKHQERVPDAQLRFLEMDGAKYVPE
                     TLESFDLVACIGASWIYGGHRGTLNALQKMAAPESWIVVGEPYWRHEPEREYLEAIGV
                     TRNDFGTHYQNVEIGREHGLEVVYTLVSSRDDWDRYEGLQWYAAETWASNHRDDPDVD
                     TVLKRVRASKTAYLMWGRDTLGWAIYVFKKGGCGPVAVG"
     gene            50493..50681
                     /locus_tag="PLM79_01415"
     CDS             50493..50681
                     /locus_tag="PLM79_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84991.1"
                     /translation="MYFDPDGDEKQYELYDLKTDPLERHNRADPENLKYYDPVEPAEM
                     QDKLRRKMEETRTTPAQP"
     gene            50766..52016
                     /locus_tag="PLM79_01420"
     CDS             50766..52016
                     /locus_tag="PLM79_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HOV84992.1"
                     /translation="MPRGAWNKTPKTRRLAASRTTCSTPSAFQVIAKPCGAACNLSCD
                     YCFYLSRERLYPGAGLRMSQDTLENYVRQHIEANCAPEVTFHWQGGEPTLMGLDFYRR
                     AVELQRKYARPGTRILNTLQTNGTTLNGEWCGFFRENRFLVGVSLDGPRARHDAYRKG
                     KGGGPTFDAVMAGIALLREHGVEWNVLTCVHAANADCRLEVYRFLRDEVRAFMVQFIP
                     VVEPGAGGEGAASRSVTGPRYGRFLITVFDEWVRRDVGRVFVQIFDVALGVWLGLPST
                     LCVFSETCGDAPAMEHNGDLFSCDHFVDPGHKLGNIGQRHLGDLVASPRQRAFGEDKS
                     RTLPRFCRQCSVRFVCNGGCPKDRSGTSPDGEPGLNVLCQGFRAFFTHIDRPMRIMAE
                     LLRKRRSPAEIMGIPHAGNLQPVP"
     gene            52306..53259
                     /locus_tag="PLM79_01425"
     CDS             52306..53259
                     /locus_tag="PLM79_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOV84993.1"
                     /translation="MKPVLFWAAFWLFFPAGIHAAGSLNGIIFIGQSNAEGQGELSDL
                     PEDLLQVPDNVEYWDKEERIYRYDQHRPRHGLEVTLLRKLSKAPMGDWLLVKYAVGST
                     SSYSWRPDWTPEIAEITDNVDQGPCYRMTMDAVRRAVMERGIAVNWRYLLVFNGERDA
                     RYLPAAKNYASNVRRVLGALRRDLHSPGMEVLICRLDLPMTKGRPYAEKVRAAQETLG
                     WVNLDGCSRMPTGTHYDSGGLRLIGLRVYRKIEQGLGFGCLPGSSADGSRHDGSIRPP
                     IVPTLPTPEFPGGCPVNGHDPGAFPADESRSQFLLGLEILP"
     gene            complement(53218..54291)
                     /locus_tag="PLM79_01430"
     CDS             complement(53218..54291)
                     /locus_tag="PLM79_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84994.1"
                     /translation="MEKIRFHRHFQRSSFHNPMMGGQLDTQELEIRRDPLTGRQSVFN
                     PGLEDKAAFFFGASDRALMERLARESEPQCFLCEDRWKRTTPTYPEKIVPGGRVVVGE
                     SVLFPNLFPVSRIHAVIRVGAGHHLPLEDFSPALVREAFLATREFVEHLCRSEEAVPF
                     LTLNGNHLGPAGASIVHPHFQVLGSDVPFTHLEELLALGAAYREKHGTSYWVDLAEKE
                     RELGLRFIACTGAVDWIAAFSPQGTNEVLGIFPGKKHFSEMERQDLCDLARGLSAVLR
                     GYASLGISTFNFTLYSGPPDAGDDSFRCFLRVISRQNFYENYRTDDYFLQKLLRNELI
                     LTTPENLASSLRKYFQPEKELGS"
     gene            complement(54367..55041)
                     /locus_tag="PLM79_01435"
     CDS             complement(54367..55041)
                     /locus_tag="PLM79_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOV84995.1"
                     /translation="MKVEKVSISLRDGKTVSGTVAAPPGNGAETGIIFAHGAGNDMNQ
                     PMIVFLAEGMARAGYLTLRFNFHYMEQGKRTPDRQDLLYDAWDAAFQFLGSHVHPRPK
                     RIFAAGKSMGGRIAAQMAARGQLAARRLIFLGYPLHPPGRKDALRDEPLYRIGIPMLF
                     FAGTRDPLCDLPMLSGVLARLSAPHRIEVIEGGDHSFKVPKAMGMSPEEIYNRILERA
                     LEWMNE"
     gene            complement(55337..57040)
                     /locus_tag="PLM79_01440"
     CDS             complement(55337..57040)
                     /locus_tag="PLM79_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV84996.1"
                     /translation="MKTTLKTTVSPVARKRRAWALFTGACLLLLAANAFADIDVARHW
                     AGTPPSIDGTFTPGEWNGATVTPLAGGQMRTKNNGQYLFMLLDFTADTTEDPVPTTPD
                     PYGGDYFVLAVDTDRNSSVTADVDFNYSACQDGREFIKAYYKTASSYTGCRETSALSA
                     GKYGFGATAASSTPHRFYEFRLDLTELGIDPATWTTSAGEMAKVRMHVSLVSQTPHLL
                     SGQPQNKIFPNLANSFYLIDLATYPGYPAGSTGPTFAGVGMVPSTYINALGNANLNIA
                     DYYAATDAPFGGKLNIFGNWKRLRTVDHARSYRVWYHNGAVWQMLLQTWTNFYFDSGS
                     GKWVPTSIGPNADGKYQIPSPSTLWYLPNLLISWQSGQVPDGFYRLRLELFDAAGRRL
                     PAPDGNLLKLNVVNTAPIPTINEVTYEGETVPACAIITQGADPAGFNFDVSVTDARGA
                     LNAFALTAIHGNNLSESIYQDNYSGHVGESGPGKWNGASNLIVPVGSKWRASETCAYS
                     FILSASSRSQNGYGLIYPYVGYHVSLTILEGAPVKTITPCGPPAGAEESSGGCKPAGV
                     Q"
     gene            57683..58471
                     /locus_tag="PLM79_01445"
     CDS             57683..58471
                     /locus_tag="PLM79_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOV84997.1"
                     /translation="MATTTLTFDVGDGIGVIRLNRPEDGNAIILEMARELLDVADQCD
                     KDPAVRAVVLTGNGRIFCAGGDLKVFAARGENASRYIEEAARTFHEALCRFNRMDPPV
                     VGAINGAAVGGGFSLALSTDIAVAAESARFSMGYTRIGLCPDGGCSYFLARMVGLRRA
                     KEIALLNPVFSARRAMEWGLVNLVVPDDQLMAAALQMARGWAEGPTRSFGETKRLILS
                     GAADSLESQMERESRAIAAMAGTADGREGIDAFLAKRSPKFTGK"
     gene            complement(58676..60322)
                     /locus_tag="PLM79_01450"
     CDS             complement(58676..60322)
                     /locus_tag="PLM79_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOV84998.1"
                     /translation="MGNVSGGQLVAKALKQEGVEAVFTLCGGHVMDIYNGCLDENIRI
                     IDVRHEQTAAHAADAWTRLTGVPGVAVVTAGPGVTDAVTGVANAFRAQVPMLLIGGQA
                     PVRKLLKGGLQEMNHVDILKPVTKFSCTVLSTERIPEIIGTAFREAFSGRPGPVFLEI
                     PQDVLDGEVDEREVHIPVRTRSQGRMTGDPKLMERAVELLSRAQRPVVLAGTQVWTCR
                     GAAQLLAFVEKARVPTYLNGAARGCLPPDSPRLFSRSRRYALSRADVVLILGTPLDFR
                     LGYGGSIGPDARIIQVDLDYSELCRNRAVEVPIQADSSAVLEELAQRVSASAESEHWP
                     TELRGREADLEAQDRGSLHSDAVPIHPLRLAREIDEFLGDESVVIVDGGDVVTMSASV
                     IRARKPGHWLDPGPLGTLGVGTPFAVAAKTAMPDKEVVVLFGDGAFGLTGFDYDTLVR
                     FNLPVIGIVANNAAWNQVRFAQLNKYGPERGNTANLLSPLRYDRIVEAMGGHGEHVTD
                     PSGIRPALERARESGKPACINVMVDREVFSSSTRNLTIYK"
     gene            complement(60381..61127)
                     /locus_tag="PLM79_01455"
     CDS             complement(60381..61127)
                     /locus_tag="PLM79_01455"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HOV84999.1"
                     /translation="MSGKCFRSLLVLAVLLSVGLTACESMKAKPSEGAGFVPMNELSH
                     REDLPFNRVWAKEGVDWKAYKTLYIEEVNVQHLLKANWWQQSLRREEMEDDVKNIARS
                     MRQRFQAAFREDPLKRFEVVNTPEPGSLTMEFALTELVPSNPVLEAMSIAAPYGSGVA
                     IQAAARGSGAKATVAFEARIKDTDTGTVLAMFADREQGKFAPINLRALTWYGEADVIL
                     DEWAEQMVQVAHKKPGEIIKASRPFTLKPW"
     gene            61545..61988
                     /locus_tag="PLM79_01460"
     CDS             61545..61988
                     /locus_tag="PLM79_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV85000.1"
                     /translation="MKILTAPRMERCIGCHSCSLACARLVHGLLSWDAAGIRISSSGG
                     LSTGFEARTCLACLPAPCVAVCPTGAYSQRKGGGVIVKKALCIRCGECARACPVDAVF
                     LDPSGGPFVCIHCGRCAAFCPHDCLEHGEVETRGPRENDVPGRAS"
     gene            61985..63733
                     /locus_tag="PLM79_01465"
     CDS             61985..63733
                     /locus_tag="PLM79_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOV85001.1"
                     /translation="MIREDFRVLVVDLAAGRGSIVRMEGVDAVAGGSGLAALLFHKYG
                     LPDRPWNDPEQPLIFAIGPLTGYFPLMSKTVCAFKSPYHDQYAESHAGGRSALSLRFA
                     DLDALVIRGRATELSCLSVGSRHLEVKDVHFLRGMDLLRTGKMLRRMYPGAGHRSIFR
                     IGPAGENLSAMACINVDTYRHFGRLGCGAVMGAKNLKGIVILGDGSFPLPPGKDYPRL
                     FAEVYGKITDTDMMEKYHNYGTPVNMAVLNGIRALPIRNLQKTSDPDMEGISGETFAR
                     DTLLRNAACAGCPVGCVHLGFVRERFMGENRYLYRQVSYDHEPIFAVGAMLQITNAFS
                     VLSLLEAVEREGLDVMSAGVALAWATEALEKEILSVRETLVPLKFGDEGSYRQAFHHL
                     GAGSNDFYRLLAQGTLKAARAYGGADFACVLGQEMAGYATGEVFFVSQALGLRHSHLD
                     AGGYSYDQKNQAPDVEGAVRFLVRDEQARVLLTSMVGCLFARGIYGDEVLSDCLRSLG
                     RTLLADNLGRVSRRVQQLRWKIRLGTGFDPRTVTIPRRFYEVETWRGRIDGTYLDALK
                     DAYARAVLELAHGGAD"
     gene            63893..63977
                     /locus_tag="PLM79_01470"
     tRNA            63893..63977
                     /locus_tag="PLM79_01470"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63927..63929,aa:Tyr,seq:gta)
     gene            64156..64232
                     /locus_tag="PLM79_01475"
     tRNA            64156..64232
                     /locus_tag="PLM79_01475"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64189..64191,aa:Thr,seq:ggt)
BASE COUNT        12437 a        19441 c        20060 g        12479 t
ORIGIN      
        1 cggcgatgtg aatcagggct cgatgatgcg cgtccctctc atcccggttc aagagatctt
       61 tgcgaagggc atctcgtttc atggcgaagt tcctcatgtc ttcggccaag cgcgcatatt
      121 ttcttgcggc ctgagcggca aggaattgtc tcagcggttt ggaaggcaaa atacgggtga
      181 gagcggctag atggcggagg caaagcccat gggattgtgc gtagacctgt tgcccccgcg
      241 gttcttcgag aaagtgggcg agatcggcca ggtaggtcct ttcaacatcc tgtagaagcc
      301 gacatactcg gcagtcccgg gagtccttga ccagactcga aagttcatcc gcaccgaaag
      361 ccgagctcga caatcgagac aattctccct ccaggcgctc gagaagtctc ggatagccca
      421 ggcaaagtcc caggggcgag gacacggatg ccaaatgcca cgaatggagc gggcaaaatc
      481 ccgtctcccg ggcaaatgcc agttgggtgt ccttctcgtg ggcaagggcg tattgccatt
      541 ttgccagaaa cgcaagggcg ctcttgccca gccgggcgca aaccgggcat ccccgggtgc
      601 ccaggtcctt ctcgatatcc gtgtcgatcc ccggaggctc cggggcgacc ggccctggtt
      661 ccttgaggtg ggctgaagag ggggtcggta tctcccggga taggtctggt cctgtatgga
      721 tggcctcccg aatggtgcaa agacgttctt cgatctcagc gagccttgcg gtgggcaggg
      781 ggcaaccggt cccccgccgg tcgaaaacgg agggaccgcc tttgtccagg acttgaagga
      841 tcgcctcccc tgcgcgggtg gcgcaatctt ccgcgcggga aaacaggggg gccaagactt
      901 cccccacgta tcgggtcgcc tctcgggtaa cggtgtcgct acagacggca aacagctgcc
      961 ggatttgtgc cttccctgtc tcctgcgtgc ttcttccggc ggcgactaca ggcagaaacc
     1021 ccagggggac gggcaagtga cggggccagt cgaagcggag gggacccaag ggagccaaat
     1081 tcccgaggtg aggatcccgg aacgtgggcc ccacctcggg catctcgacg cggtccaacc
     1141 cgaggacctc tgcggcaacc cgagggattc gccccaggtg cagttccagt gtcgacgcga
     1201 tctgctgcag ttttgccgag aagtcgggct tgagatgttc cagggactcc gccatccacc
     1261 cgtccaggtc acaggccagg cggagcttgg aataacgaac caccctctcc aggaccttgc
     1321 ggcgaccttg ccggggatga cgctccagga gttgtttcat ttcacgaaac aactcgtctt
     1381 cctttgactg gaagaacccc tgtaacggag cgaccaggcg ctccgtcaac caggcggtca
     1441 gttgtctgcg gctcaccgtt tcgagatcgg tgcgctcttc ccgcaactgc cccaaccggg
     1501 cctcgagttc cctccgaatt gccgcaatgc cttccacgga tcccctggag gcaaattctg
     1561 caagccgggc cgccgtctcg gctccggatg cgaggcggag tgcccggtcc gccatggcgg
     1621 ccagaaaagt gcttttcctc tcgtaagcaa gaaaccttgc caggtgactc ttcaaatccg
     1681 ccattccact cagggcatac ccggcggtat cctgccggat actcgcttcc atggccagtt
     1741 tgctggagac cggaaagagc cgaatcgggc caagctgcat ctcccgggaa agaacctctc
     1801 ggatatattg aaggatttcg tccaattccg ccttgtttac caggtccgtc ttgttgacca
     1861 caaaaaaaat cttgccgacg taacctaaaa cggtacccag aaaatcgagt tccatccttg
     1921 tgaggggggt atccacggca gtgacaaaga tcgccgcatc gcaatggggg aggaaacggc
     1981 gggcggcttc ggtattggca tccgtcgcgg aaccgatgcc gggtgtgtcc acaaagtcca
     2041 ggcccgggcg aaggaattct gcgggtgtct cgacatgggc ccgaacaact cccttggtgt
     2101 tgccgggatt gcccttttcc gtggcgtatt cgaaaaggtt cgaaatgggg aaactctcag
     2161 gatagagacc gttcccacgc cggatgacca gcctttcctg gggaccgtga cggacgttga
     2221 cgattacgga agtcaacggc aagacgccgg tgggcaaaag gtcccttcca atcaaggcgt
     2281 tcatgatgga gctctttccc cgtttgaatt gccccaccac cgccaggttg aatcggtctt
     2341 ccgcgagcct ctccatgagt tcccggcatt ccatggccag ggcgcggctg cctccttcgc
     2401 gatggaattc gcccaaagaa cgaatctctt cggctatggc gtgcttgatc cgacggtact
     2461 cctccaagtc ggcaagcttc tcctccattg ccgctttcat gggaacgtcg gccgcttgac
     2521 gcacagattc cactttttgc acaagggatg tcgcacccat gtcgtgaaac ctcaaccgtc
     2581 tccgttgccc ttcgaggtct cccctgacgg aagaaaaaaa acctggagtc gttcgatctc
     2641 cttgatttcc tggatgaccg ccggagtgcc gtagacattc tcgaggatct cggggcggag
     2701 cacggaccgg gtttccccgt ccgcgaggat ttcgccgttc ttgagcatga tcagccggcg
     2761 gcagaaaagg gctgccaggt tgacatcatg gagggccgca aggatggtca ggttctcttc
     2821 tcggttcagt cgttcgagtc cctgaaatat tttcaatttc ctgtggatgt ccatggccga
     2881 ggtggcttcg tccaacagca gtaggggtgc ttgctgggcc agggcccggg ccatgattac
     2941 cgactgtctc tcgccgccgc tcacctcagt gataagccgg tcggcaagct ccgtggtgtc
     3001 cgtgcggtcc agggcccact ggacggcggt cgcatcccgg atcccgtttg tgcgaaagaa
     3061 ttcctggtgc ggatagcgcc ccataaggac gacttccccg catgggaacg gcaggcgaac
     3121 gtcgcagtcc tggttgaggg cagccatgcg ccgggctctt tcctttggtt tccattgcga
     3181 taatggggaa ccgagaagcc ggatttcccc gctccgtagg ggaaggattc ctcccgcagc
     3241 caggagcaaa gtgctcttgc ccgccccgtt cggtcccaac accccaatga tctctcccgg
     3301 ggcgacccgg aagctgatcc ctttcaaagc cgtgtgacgg ccatacccgc accaaagatt
     3361 gtgaacctcg agtaagggtt gggccctgtc cgccccggaa ttggggcggg tacggccggt
     3421 tgggcccaga tcgggctcct gtttcctcgt gacggcgaag ggtcccattt tgtccttgcc
     3481 gggcctccct cgacccatcc cggctggata aggcggacgg gatccggagg gaaaatccat
     3541 cctcttagag gggacttcgg aagacggctg cccgacccag ttctgcctcg atctcgagga
     3601 gccggttata cttggcgatc cgctcgctgc ggcaggcgga tcccgtcttg agttgggccc
     3661 ctcccatggc caccgcgaag tcggatagga aagtgtcttc ggtttccccg gaacggtgag
     3721 aaatgacaaa gccccatccc gccttacggc aaaggtgaac ggcctcgatg gtttcggtta
     3781 cggtaccgat ttggttcagc ttgatgagga cggcgttggt ggttttttcc cggatccctc
     3841 gctcgatgaa ccggctgttg gtcacgtaga tgtcgtcccc gacaatctgg atccttgtcc
     3901 ccaaggcggc cgtgtgttcc tgaaagccgc tccagtcatt ttcatccagg ccgtcctcga
     3961 tggagacgat gggatacttg tcgacccatg tccggtagag gtcggtcaac tgggaactgg
     4021 tcatccggcc aagcccggac ttattcagtt cataaaagcc gttctcgaaa aatgaactgg
     4081 cagcggggtc gagggcgatg gcgatgtctt ttccgggttc gtacccggcg gcggcgacgg
     4141 cttccaagat gagctcacaa gcctcttcat tgctccggag gtcgggtgcg tacccccctt
     4201 cgtctcccac ccccgtggcg tatcctttct gtttcaggat tcgcccgagg gcatggaagg
     4261 tttccgctcc atagcgcagg gcctcggcga agctgggagc gcccaagggc atcaccatga
     4321 attcctggaa gtcgacgctg ttgttggcgt gcttgcctcc gttcaggatg ttcatcatgg
     4381 ggacggggag ccttgccggt ccgggaccct gaagatactg gtaaagtggg aggccgcagg
     4441 cctcagcagc agcacgagct acggccatgg agactcccag gatggcattg gctcccagtt
     4501 tgcccttgtt cggagttccg tccgcctcca tcatgatccg gtcgatttcg acctgccgaa
     4561 tgggctccat ccccacaatc aggggggcga tcacctggtt gacgttctcc acggccttga
     4621 gaaccccttt cccgccgtaa cgggccgggt tgccgtcccg cagttcaacc gcttcgtgct
     4681 ctcccgtgga cgccccggaa ggcaccgagg cgacggcttt catgccgttg tccaagcgga
     4741 ggattacccg gaccgtcgga ttgcctcggg aatccaggat ctccaaggct cgaatgtcgg
     4801 caattctttc cttcatgttg aatcctttcc gtgtggatgc gaagaatccg gggcccggcg
     4861 ggtcttgaga ggccccaaag ctttccgcgc caggtctcga attctcccgt cagaagattg
     4921 aagaaggctt tccaggaaag ccacggcttc cgtcgtcccg atctccccca aagcaccaat
     4981 ggcggccccc gccacaaaca gatccttccg atcccggacg agtttgatga gggcgggtac
     5041 ggcccgtggg tctttcagcc ttcccaggag ccgggcggcg cgtatcggcg tggtcggttc
     5101 ggggtgacgg agggaaacga taagtttttc cgcgtagtcc ttggaatccc agaatcggtg
     5161 aatgtcgagt ccacaagacg ggcatttccg aaagtcttct tgagaatcct tccagcaccc
     5221 cgggcaataa tatcgtacca tgccttccct cgggtccgca gctcctgtta aggacccccg
     5281 atcgcttccc cggatcgacg gattttcctt cgaaagcttt ggcgaaatgc cacgggcggc
     5341 gcggagtcat ggggctggac gacgggtgac ggcccccgag ccggggggag ctttctttgg
     5401 taaggtcggc tttcgaggca ttcgcactcg ggaattcaag cgccaccaaa aaagctcccg
     5461 aagactggcc tccgcaagag ctctctttca cccttcacca ctggcggctc ttaccgcggg
     5521 aagcccgccc aggcaagagt catcaaccct ttggggggtg gttccggtgg actctatcac
     5581 cgatcgcgtt cttttccata aaaccacatc gggtcgtgcg aatcaaggga ttcctggatc
     5641 attcgcgccc cgggtctcct cccgcctgcg gccaaaggca gccgttccat gccgaggcat
     5701 gggccggata agcggtcggg ggacctcttc tcgccttgtt aacgcaccac gaccacggtg
     5761 cactgggcgt aattcatcac ccgacgggcg atggagccca gcctccattg ctggaggaaa
     5821 ctcccgcctc cccggtgacc catcacgatc atccccgcct cttcctcgtt ggccagaatc
     5881 acgatctgct ccgccggatg gccgattctc acctcgaagc ggggctcgac cccctggaca
     5941 aaggcctggt ccttgaggga ggcaaacctc gagcggtaat actcggtggc gtgttccagg
     6001 accgcctcca tctcgacggc gatgggaggc tcgggaggcc gggcgacgga gaccaccacc
     6061 agttccgccg tgtatcggga cgccagatcc agggccattt cataggctct ctccgactcc
     6121 ggggaaccat cgtacgcaat gaggattcgt tgaatgttca tgttcaatct ccttgggttg
     6181 cgagctcccc gctgcgagcg ccctccacgg caggggtccc gcgcttctct cccggttgca
     6241 ggaggtgacg gggaaggtag taggcgttgg cgatcatcgt gggcaccacg gcactggcga
     6301 tgaccgtggc gaccagcaac gaatactggg gtcgatcgat gatcccgtgg gtgagaccga
     6361 acagggccga aatgctcccg aaggtcaatc ccgtggacat gagaagagtg gtgtacattc
     6421 cttcctgacc cgagctccgg aacattcgag taacgggata caccgcgacg aacttcgccg
     6481 ccaccttgcc caggagcagc aagagaaaga tcaggggcgc tccaaggagg gccgggaccg
     6541 agacgaacgc acccgcccgg agaaagtaga agggggtgag cagtcccagg gtcagggtcc
     6601 tcaggcggcg gatgaggacg tgatctctgc ccacggtccc cgccagcacc atccccacca
     6661 cgtaggcggg gagcaccgct tcgcttccgg cccacagggc caatcccccc attccaaaca
     6721 ggaccaggag aaggtacttg gcttcgattt ccgaaggtct tccgccgtag atgccgaaga
     6781 accggggcgt ggcccagggc agaagaacca ttccaacgag ggttgccagg acgaaaaaga
     6841 gggttttggg gctgaacggg gcaaagaggg accccagggc caacacggtg cccaagtccg
     6901 tgatgaagca ggccgccaga acggtcttgc cgaaggtggt ggtgtttaga cgaaactcga
     6961 tcatgaccgc gtagaccacg gccaccgacg tggtggaaag ggcgaccccc gccagccagc
     7021 tggcccgtgc gtcccagtgg agaaagtaat aggccatggc cgcacaaccc acgaaggggg
     7081 caagaaagct cgccaatcca acggcggaag cttccttcca cttgagcttg aagacctcgg
     7141 ggtccagttc ggcaccggcc aggaaggtca gcatgatggc cccggtgccg gacaagaact
     7201 tgatccaggg ttcgtccagc cccaagaccg ccgcgcctgc catgcccccc agcaccagtt
     7261 gggccagggt tcccaccagg atctcggaca aggcggtggc aattcgaaac catgcggaca
     7321 agagcgcggc aaacaacgcg aggcccagcc agagggccgc aagaaaccag gtttccccca
     7381 tctcgatttt ccctttcctt tgtgatttta cggagggatt ccacatcccg tccgccggtc
     7441 ccggaggcag gtcgccggga aacctcttcc atgccgcacg gcacagtgga agcctgccgg
     7501 gttgaatcct catagagcac cgcgagcgga ccgagcatca caaggaacat gggaaaacga
     7561 gatttagacc ttcttggagt tgctgccgag gaccagggtt aaagaatgcc gtaaccgaat
     7621 caacgagaca aaacgagaag cctcccaatg cccgtcaccg ggaattcgca aacaaaaagg
     7681 ctccggcatc aagttcctgc aggagccttc gggatgattg ccgtggaacc cgaccctgcc
     7741 ggaggcacct gcctccccaa gagtcatcaa cccgtatcgg gtggttccgg tggactccat
     7801 cacctgttcg aattggacgc cgaaccggat atcacagccc aatgcccaaa atcaagaggt
     7861 cgttgagtgc acaatcttcc taagagcttc ccccttaata aaattgcaca ttgacggaac
     7921 ccgccgattc cagtattttc ttgcacgcgc aggttgccca tggtgatgaa gttcgccgga
     7981 accgcccaca gacgggctga tgacttctac tttctttcct aggagagtgg gagttttttt
     8041 ttgctttttt catgcacgga ggggtgaaag atgctcgatt atccgaagat cagggacggt
     8101 ctgttgcaag tggtggatcg actctcggac acggtgagga gcgaaacgtc aaactctctg
     8161 aacctggtcc ggtccaagct cgaggaggaa gccttcaacc tggtgattct cgggcagttc
     8221 aagaggggga agtccacttt catcaatgct cttctggggg aaagcattct ccccacggcc
     8281 atcgtgcccc taacctcggt ggtgaccatc ctccgttacg gcccgactct gcgggtggag
     8341 gtccggtatt tggacgatcg ccgggaggaa atccgccttt cggatcttcc ggcctacatc
     8401 accgagcgcg agaaccccga gaatcaaaaa ggggtgaagg aagtgaccgt gttctacccg
     8461 tcggaatacc tcaaaggcgg ggtccgcatc atcgacaccc cgggcgccgg ttccgtatac
     8521 agccacaaca ccgatgtggc ttacgggttt ctgccccagg tggacgcggg gatcttcgtc
     8581 atttccgcgg atccgcccat gtccaagagc gagcaccagt tcctcaagga cgtgcgccag
     8641 tacgtggaca aattgttctt tgtactgaac aagatcgacc aggtaagtct ggaggaccgg
     8701 gaggaatccc tgtcgttcac catgcaggtt ctggaggatg acctaggcac cggaaaggtg
     8761 aaaatctttc ccctgtcggc ccggtgggcc ctcgaaggca agaaggccca ggaccgggaa
     8821 atggtctccc gcagcctcct gccggacttt gaacaaaggc tccgggagtt tctgctccac
     8881 gacaagggcc gggtattcct atcttccatc atcaacagcc tccggaagct gatctcggat
     8941 gaaaccatct cgtttcggtt ggaacaggag gccatcaagt tgcccctcca agtcctcgag
     9001 gaaaagatcg cccggttcga agcggaaatg gctctcatcc agaaagaccg ggagaagaac
     9061 cagtacctgc ttgcgggaca tttgaaaaag atgaccggcc agttggacga agagatcgga
     9121 gaattcaagc ggaaacaact ccccgcactg ctgaaggagc tcgaggaaga ataccttcgc
     9181 aggactgggc ggggaggggg caacctgcgg gaggagctcg aacagttcgt cttcgatcga
     9241 atccggcaga gcttcggcac ttggaggatc cagtgggccg agaagatctc ggttcgcctc
     9301 gaagaggctc accgggagtt tgccgaaaga atcaacgaaa ccatcgaccg gatcctggcc
     9361 ctcaccggca accttttcga actcgatctc aagccttttt cggccgtgga atccctcagc
     9421 accaaaagcg atttctattt tctcctcaag gatgatcccg tgggcctcga gctcatccag
     9481 ctggcggtga cctcggcctt accccagttc ctggcaaaga agatgatctt gaaaaccatg
     9541 aggacccagg tagaagaact cgtggatcgt cactgcgggc gagtccgcta cgatatggtg
     9601 aaccggctcg aacggaccgt ccgggacttt cagcacgccc tcgacgagaa gatcgatctc
     9661 accctggagg gaatacgaat ttcgttccgc aaagccctgg ccctgcattc gaaaggtgcg
     9721 gaggacatga cccgtaacct gggggagctg tcggcaagac tgggttccat ttcccgtata
     9781 cacgaggagc tgttggctca tgccaccgtg gtgagcgacg gggttgggga gaattagggc
     9841 aggcaagata gtgccgggga ggcgccgaat cttcccgcct cggggcggag aagaccgagg
     9901 aaggggtgcc cgagtgcgga tcggtcttaa gaaacctcgc aggcgcgaag gccctggagg
     9961 atttccccgc cgacggcctt taccccccgg ccgatgaaga ccagccgggt ctggtccgag
    10021 gaaaactctt cgagatccga ccgccccgcc acataattga agagaaaacc tctgccgtcc
    10081 aatcgaacga atcccttggc tcgatacacc tgggagggca ggcggcggac cagctcccgg
    10141 aatcgttgct cgcccaacgg ttttccggtg gcaaaagaaa cggattcaaa ggtcgtcatg
    10201 tcccgaaaag ggccccccat cctgggacgt ctctccactt cgaggccgaa cagcaggttg
    10261 gtgtccacct cgcatcccgc cgttcggaag aaaatggccc gggggttgta ttcgcgtacg
    10321 gcggtttcca cctgttccgc ctcttggagg gaaatgaggt ccgtcttgtt gatcaggatg
    10381 atgtcggcgg attcgatgtg agagcgggcg ctcatcccga tgtaggggta catggcgacg
    10441 gcgtaggcat ccacgacgta gacgacgctg tcgatgcgaa cctggggcat tcctccgccc
    10501 acccggagaa ccagggcgtc cgcttcagcc actcccgagg attccagcac gatgaattcc
    10561 gggcggatgg tttccaccag ttcccggatg gccgcttcaa attcccccgt catggaacag
    10621 cacacgcagc cccccgatac gtcgatgatc ttgagatgat cgccctcgag aatcttgctg
    10681 tcgatggaga cctcaccgaa ctcgttcata atgacggcga tccgttgacg ggcgttctgc
    10741 aggatcctct tgagcagggt ggtctttcca ctgcccaagg ttccggtgat gagcatgatg
    10801 gggaccttgg gttccatgaa aggcggttcc tctcgatgtt gatgtacatc catccggtgg
    10861 aaacgctctc acctcttgcg ggaccgaggt tcgcgtctcc cccgtccttt cgtcccatgc
    10921 cccggggggc ctcagggtcg gggtgcgacg cactgtatgg gttccgagca cccttgtcaa
    10981 ccctgtgtga tctgcccgct ccgtgggcgt cccaaccttt tcggcgttcc cccagatgga
    11041 aatgacgaaa caggacgcct accaacccaa tcattcaatc catgtgagcg ttgccggctc
    11101 ctgtcccccg tgggctcatg agcccttgtt ttccttcgtt ggaactcgtg accgagatca
    11161 catacaattt gatgaaagat aacgattggt gtatcctaac ccacaggttt cggcggacct
    11221 ttttaggaat tgcaaggcgg tgcattcctt tcccgaagga ggactctcat gattggcaga
    11281 ccaggccgat ggaccgcact caccgtatgt cttgttttca cattttgcat agggatcgga
    11341 gggttggggg caagcccccc ccgggacaag gacccggttc ccgttccgcc ggtttccgac
    11401 accttgggga agctcccggg gaaagcgtgg aaagggccgg gtgcctccag gatacagaag
    11461 gaaatcgacc agccgaatcc caaggattac cgcaagatgc aactgcgtcg gcaactgcag
    11521 gaagccctgg atgaagctgc aaggtcggaa gaggaccggc cggaagtgag ggcccaggcg
    11581 gaaaggctgg ccaagacggg gaaggacaag gtcctggtaa tcctggtgga gttcggcggg
    11641 acggatgtct tcacctggac ccagggagtc tccacctggg accctctcgg gaaagcggat
    11701 cccaacgaat ccacagggat cgccggggat tgctccaaga tcatcacgtc caccgccgtg
    11761 ttttcctata cgggtccttt gcataaccag atccagcggc ccctttccga atccgaccgt
    11821 tccggggaca tgatatggac ggaagatttc gattcctcct actaccaggg aatcatcgac
    11881 ggcaacgggg ttgtcttcga ctatgtgcgg caggacaaca gcacggtcca cgaggacttt
    11941 accgggaaat ccgttcgaac cttcctggag gaccagtccg gggggctcta caccatcgag
    12001 gcggacatct acgggtggct ccaggttccc cactccatct ggtggtacgg tgcggacccg
    12061 tgtcccgggg cccgatcggg tctcgatgtg gggcacaatg gggccattcc cggggcggga
    12121 aacgccaagg ccctggtaag agacgccctg gaccagctca aggccctgca tccggaaccc
    12181 tccttctggg cccagtatga tcaggacggg gacggataca tcgatcgttt gtggatcatc
    12241 cacgcgggtc tgggagaaga ggacagcacg accctactca accgaactcc ctatggggag
    12301 ggcgggctct ggtcccattc ctcgagtctc ggctcctatg aaatcgtgca gggcatttac
    12361 gcgggaccct acatcatgat gcccgagaat tgcggcattg gcgtgctggc ccacgaatac
    12421 gggcacaatc tgggggccac ggatctgtat gcctacgggt tcggggagac ctcggcggga
    12481 ttctggactc tcatggccga tgattggacg gggtatccca tcggctttct gcccccgaat
    12541 ctcgacccct atcatttgga cgccctggga tggctcaatc ccctcaccgt cacggacccc
    12601 acgaagatct acaaagtgac cgtggggcag gccggcaact tccccggggg ggccggggtc
    12661 taccgtggag tgaaaatccc tctgaaaaag ggcgttttgc ctttgccggt tcagcccgag
    12721 gggagctatc aatggtggag cggtcagggg gacctggtta acgccaccat gacccttacc
    12781 gataccatcg ccatcccggg cggcggctac agactcaagt ttgatctggc ctatgacatc
    12841 gaacccggat gggacttcct ctgggtgcag gtgtccgggg acggcgggaa caactggacc
    12901 accctcacca acggcaacac cacctgtgcc cacgatgctg actggatcgg gggcgcttac
    12961 gggttccccg acgacctgtg tgctgcggga atcggcggtt tcaccggcaa taacgggagc
    13021 tatcccgcct acgaggagca aagtttttcc ctggggaatt gggccggcca agacatcctg
    13081 gtgaggttct ggtacatgac cgattggaac accatgggaa atggggcctt catcgacaac
    13141 atcgccatca cctccgacga gggcgtcccg attttccagg atgacggaga agggggcggc
    13201 ggcaactgga actatgccga gggattcgag ctcaacaacg ggacccggac cttcacccac
    13261 aacttctact tccaatggcg aaacgtcaac gatcccgaca gcttcgacag cgccctgggc
    13321 gattcccgtt ggcggttcgg tccggccaac acggggcttc tggcctggta caacaacaac
    13381 ttttacggag acaacgaagt ctgggatcac ctgttcgacg atttcggctt cgggcctaag
    13441 gggttgatgc tggtggcgga cgcccatccc gaaccttacc gggatcccta ttatgtcggc
    13501 gtggggtaca acaacgaggg cggcaacgtg gatcatcgct ccctcatgag ggatgccccc
    13561 ttcagtctgt gggattcggt ggatttcacc atgcagcctc cctatgtgta ccaagaggcg
    13621 agtttcagcg ggcgcccggc cgtgagcgtc ttttccgatg acgtggggta ctatcccgga
    13681 tcggagtatg tttcccgagg accggggtac gatccttcgg tggcgatgaa atgggtgacc
    13741 caccagtggg acgccggagt ggtgatgccc tcccgagtgt tctacggaat ccgggccccg
    13801 gggtatacgg gcgaccagga gtttcgtttc aactgctcga gactcgccgg agggcgcttg
    13861 ggctgttact ggttgggagc cggggtgggg ctgggctatg acggatccaa cggaaatccc
    13921 ggtgaggtct ccggccaata cgggtggaaa gcccagatcc tgagccagac ggaatacacc
    13981 gcgaccctca aaatctggaa ctcggtggtc cccgtggccg attttagtgc cgacgtcgtc
    14041 aaggggaagg cgcccctcga cgtgaaattc accgacaagt cccggggaga gatccaaacc
    14101 tacgagtggg atttcggcga tgggaacacc agcaccgaat ccgatcccac ccacacttat
    14161 tccaagggtg gaaagtacac ggtgaagctg acggtgaagg ggccgcacgg gaccgacacc
    14221 aagacccgga ccggctacat ccaggtgtac gtgcctccca cggccaattt cgtcgctgcc
    14281 gcaaccaagg gcaaagcccc cttccgagcc aagttcgagg acaagtccaa gggagtcgtc
    14341 accaaatggc tttggaagtt cggggacgga gccaccagca ccaaggaaaa cccggcgcac
    14401 gtctataaga atcccggacg ctatacggtc aagcttaccg taaaggggcc gggtggctcc
    14461 aataccaaga cccgcagcaa ctatatcgtt gtggtgaagt agtcaacagt cccgaaactt
    14521 cggaatccgc ctccaacggc ttcctcccgc cccgggcggg aggaagccgg gctgtcgttc
    14581 ccgttctttc cccaaggttg gctccaagcg tgctctagaa ttgagaccgg ttaagaaaag
    14641 tcccggggtc gtggacggct tcgttcaagg aacaattccg cggaggagag aagacccttt
    14701 cacctcgaca tgagggcgaa ggaagtttaa tgtatggcgg tgacgttctc ttccctcgac
    14761 gggtgaaaaa ccgatggttt ggggggttgg acggagaggg acgggtcttc aaaaggattt
    14821 tcgcacggat gcttccccgg atggtaaagc tctctgcctt gggtctggtg gtcctcgttt
    14881 tggcggggtg tgcccggggc ctgagctgca gtcgccccga cggtcggttt atgttcgctc
    14941 ggacccgggt gcagcctgcg gaacggctcg tttctgcggc gccgtcccta tcccaggacg
    15001 tctcggtgga tttggcggaa acgacccaaa gcctggcgga acaggaatcg gctttggcgc
    15061 agaaggaggg ggaggctcgg acggcggccc tgtgcgagtt ggcccgcctc tgctttcttc
    15121 ttggcgaata cggggagaag tcggaaagag aggcccattt cagcaaggga cgaacctttg
    15181 cggaaattct ccgtcaagag gaacctcgac gggtagaggg atactactgg ctggccctga
    15241 atcttgcagg cctggccgaa gccgggagcg ccgcgcgtgc tttggggatg gttcccacca
    15301 tcatcgaaaa cctgaaaatc gccgtcgaga ttgacgagac ctacgaccag gccgggcccc
    15361 accgggtcct gggccgcatt tactgcgaag ctccctgctg gcccctgtcc gatggggacg
    15421 tcaagaagtc cctccatcat cttcgccggg cggtggagat cgccccggac aacagcacca
    15481 accaccttta cctggctcaa accttcattc agctctgcga actcaaagaa gcccacaggg
    15541 agctgcagca ggtcctctcg ggaacgcagc acgccacgtg gccggcaggc ctccaggaag
    15601 atcagctgga agccctcgat ctcctgaaac gatgcggaaa acccgactga tcgcccaccc
    15661 cgcctcgatt cgctccccct gcggatagca ccccgcccgc agcaagatcc tcggcccggt
    15721 cgtttcctca gggccaggcg aattgaacga tggagaaatc atcttccagg gattccttgc
    15781 cgcgcatctg ccggacatgc tgccagagcc gatccaggca cgcttcgccc tgcccccccg
    15841 gggtgcaggc tccttccatg aactccacga acccttccag gtcccacatg gatccatcgg
    15901 cgcactgaat ttcatagacc ccgtcgctgt acagatacag gcggctcgag gccgggacct
    15961 cggcgcttgc ggactggaag ggatagtccg gtatgcaccc gaccatggtg ttcgtgttgg
    16021 tgagcagggt cgtttgggtg ccgtcgccgc cggcggaggg agagatcagg agtgcgggag
    16081 ggtgcccgcc gccggcaaac acgagctccc gggaggaggg gctgtagacc ccgtaccaca
    16141 tggtgaaaaa catgtccttg tggttttcca tgctaaatct ttcgttgagg gcggaaagga
    16201 cctgggccgg tcttcgaaag tcggcgtcgg caagggtttg agtgcgcaaa acgttcatga
    16261 ccgataccga aagcaaggcc gctcccacgc cgtgtccgca aacatccagc aggtacatgg
    16321 cgaagtgatc gtcgtccagc cagtaataat cgaaggtatc tccgccgagg gatgcggaag
    16381 gaaggaagcg ccaatcggtg aggatcggtc cccgggaaat gggcgcggga agaagttttc
    16441 gtacataatc cgcggcttcg ttcagttccg ccgccagtcg cgtcatgact tcgtttcgct
    16501 ggaggaaact aatgtagccc ctggaatgat ggcggatgcg ggcgatgagc tcgatcttgt
    16561 cggggagctt gaccaggtag tcgctggcac ccaggccgaa cgcccgggcc ttgacgatgg
    16621 gctcttcttt cgaggaaagc acgatcaggg ggatgtcccg ggtctttgca ttggccatga
    16681 agtaccggac cagggttaga ccgtccatct cgggcatgac gaggtcctgc aggatcacgg
    16741 tggggcgcag cgtcacggcc gcctgaaccg ctcgggccgc atccgggcaa tagtgcagtt
    16801 cgatatcctc ttctcccttg agcatgcggc gcactgcttc ggccacaatg ggctgatcgt
    16861 cgatgaggag cacggtgatg gttcgctgtt gcaggtgggg atctggatgt ttcgggtcca
    16921 ttcgctttct ctttccggtc ctttccgaat tccgtggttt cgagctagag cggtttcctg
    16981 ggagaaaact tggtcgtgct gcgggagggc gggtccttga gccgaagggg attcatcttc
    17041 aagatggctg cggggatctt cgacaagggc aggacctctg ccgccgcccc caattcgatg
    17101 gcggctttgg gcatgccgaa caccgtgctg gatttctcgt cctgtgcgat ggtgtgccac
    17161 ccggcccggc gcaagtgcaa gagcccccgg gcgccgtctc ttcccatgcc cgtcaggaga
    17221 atggcgagcc cctttgcttt ccagtgcgct gccgcgctat cgaagaagac atcgatggaa
    17281 ggtcggtaga cggcatcaag gggctccggc gagtaggaca atcgaaggtc cggtcccagg
    17341 acgagatgat gctcgtttac ggctacgaga accactcccg ccgccggttt ggttccttct
    17401 tcggcgcttc ggacctggac ggaggtctgc ctgcggagcc aatccaccag tttcattcca
    17461 aagtccgcat ccacgtgttg gatcaccaca atggccgggc cgaagttctc gggcagggct
    17521 tcaagcaggc ccgccacggc ccggggcccc ccggcggaag caccgatgac caccaggggg
    17581 ggaagcagtc cggtggggac ggccgggtcc ttgcccgtct ccaccggcgg cgccggggag
    17641 ggtttttgaa tgaggcgggc aattcgggcg atctttgcaa ggagctcctt tcctccgcgg
    17701 atttttccct ggggaccgag gacgggggtt gcgatgacgt ccagagctcc ggcccccatg
    17761 gcgtcgaata ccttgtccgc atttcccata acggttgccg tgacgatgag gatggcgcac
    17821 ggcgtgtccc gcatgattct ccgggtggct tcaaccccgt ccatctccgg catgatgagg
    17881 tccatgagga taaggtcggg caaatcccgg gagcaccggg cgacggcttc ggacccgtcc
    17941 cgggcgatcc aggcaagttc gtagcccggg ccgctgagca cgacccggcg caggatctcc
    18001 acggccatca gcaggtcgtt gacgatggca atccgcaacg atgcttccct tcccccggca
    18061 cgtcagccgg gcaaaccgat gaggtccgct acggcgttga cgtaggtctc gtcgtagaag
    18121 ctgctcttgg tcagatagta gttggccccg gcctcgaggc ccgccacccg gtctccctcc
    18181 cgatccttgt aggagagaat gatcaccggc agtcggctca gctgagggtc gctcttgatt
    18241 ttgcgaacca actcgaagcc gttcaagcgc ggcatgtcga cgtccgatac gatcaggtcg
    18301 tatttccccg ttcgcacggc gttccatccg tccatcccgt ccacggccac atccacctgg
    18361 tagcccctgg ccgccaggag cctcctttcg acttcccgga cggtgatgga atcgtccacg
    18421 accaggatgc gcttgaccgc cgtttgcggg gcggcgactt ccgaccgggt gatcttaaca
    18481 atcctgcctc ccgtaagaag gccgtccacg gaacgaacca ggtcgtccac gtccaggatc
    18541 agtacggggg caccgtcttc gctcaccgcc gcggcactga ggttggggac cttgcccagt
    18601 cgagggtcca ggggccgaac gaccagatcg acctctccca gcagtcgatc caccacgatg
    18661 ccgtacgagt tcaggcgatc tccgatgagg accaccgaaa gctccgagtc cgtcaccggg
    18721 atggggatca acccgagagg ttggctggct gccacgatcc ccacgttgcg tccctcgaga
    18781 gaaaaatact gctggtcctc gatgagacgg atctcctctt ggggtaccat tctcacggca
    18841 tcgatgcggg tcaggggaaa ggcgtagagc tcacgggaga tctccaccag gagggcccga
    18901 atgaccgaca gagtgagggg aagttcgagc tggaagctgg ttccgacgcc gggagcggat
    18961 tgcacccgga tggttccctg aacctcgtgg atcatctttt tcaccacgtc caggcccacc
    19021 cctcgtccgg acagctcggt aatcccgtcc gtggtggtaa acccgggcag aaacagaaac
    19081 tccatgagct ccgcgtcgct cagggatcgg accacctccg ggggaaccat gcccttggcg
    19141 gccacgacgg accggattcg ctcggggtcg atcccccggc cgtcatcgtt caccacgacc
    19201 accaacattc ccgcccgatg ggcggctttc agttcgatgg tgccctcctc gggcttcccc
    19261 gccgcccgcc gctcggcggg ttgttcgatt ccatggtcgc aagcgttgcg caggatgtgg
    19321 gtcagggggg cctccaacct catgaggacg tcacggtcca ccttggtgcg ttccccggat
    19381 gtccggagcc ggatcttctt gcccaggctt cgcgccaaat cccggaccat tcgcggaaac
    19441 ccgtggatac cgtctcgaaa cgggcgcatt ctcatggcga gggcttcatg gtagagtcgg
    19501 cccgagaggt tctcccaacg gagcagatag gtatcgaaaa actcgatctg cctcgatagg
    19561 ttgtcccggc attcgcccgc catcttgatg atttccgcca gcacctggcg gtcccgggct
    19621 tcggaatcgc cctcgagaga agagggtctt ctccgttcgg cggtctttgt caaccgaatg
    19681 agcaaggact tgagttgctg gagctcctgc atgcgggacc gcaggacctg ggactcgatc
    19741 aggcactccc ccgtaaggcc gatcattctg ctgagactct cggcgctgac gggaacggcg
    19801 tcctcgggtc ctccatgggc gggaaccgac ccaccggctg ccggggggcc cgtttgggcc
    19861 tcgatgcggg caggttcggg ttccggggac ggggccgaga cggtggcggg cgtcgggtcg
    19921 ggggctgtga aaagtaacgg ccccggggaa ttctcttccc cgggtggcag atcggaatcg
    19981 ggaggagggc cggagaggag agcgcgaatc ccttcttcca tctcaaggac cgcaggttca
    20041 ttttcccgga tccaggaaag gatctccggg ggctgcaccc ggctcaaccg tttgaagaca
    20101 tccacgccct tgaacaggag atctcccatt tcgggattga gaatcctctg ccgtgcctga
    20161 aatgcaagaa agaggtcctc catggcgtgg gccagtcgga cggccatgtc caggcccagg
    20221 attcgtgccg ctcccttgat ggagtgagcg gcgcgcatga gggattcgag gactcccgtg
    20281 tccgaggggt tcccttcgag gtccaccagc cctttttcga gggtgcccgc atgcatttcc
    20341 gtttccatgc gaaacagctc gagcatgccc tcgtcggcca gtatgcctag gtcgccgccc
    20401 gccatcgggt actcctgttc agggcgttga agagcaggcg ctcgtccaga agggccaccc
    20461 gcttcccggc cagatcccgg atgccgcagg aatagctttc atgggaccga gcgacggtct
    20521 cgggcacggg ttccagctcc gagaggggga atcggtaggt ccgatccact tcatccagtt
    20581 ccaaaaccca gcgggagtct ccgtcccgca cgacggccat gcgccttgtt tccaacggct
    20641 cctgccgggt ttggggtctc gatgcttcct cgagccccaa gagttggtgc agggacatgc
    20701 acaggaggag ctctccgcga acgttgacca ggcccagaag gacggagttg ctgcagtggg
    20761 ggatggtgtg gatggaacga agggtcacga tttcttcgaa acagcggctc cggagagcga
    20821 accactcccc gccaatgcgg aacaccacca cggacacctc ccgggaggtc tcctcgcggc
    20881 tcgccttcgc caggagcgaa gtccactcgg ttcggtaccc ggggggggga gggcggtcca
    20941 gtagactgcg acccaggcgg gaatagacgg ggcagttgcg gcagtggatg aagccctcca
    21001 attccccgca cgtaccgtcc ccggcaatgc cgacgaccct ccagcagtcc ggggggatgt
    21061 tctcggtccc ctcctcctgc cgtcccgcgg gaagggcaag gtccttcgat gatggctccc
    21121 gaaaacgatc cgtcaaccgg acatctccct tcgtttgatc cgttccatgc gttccctgca
    21181 tcggtcggcc ccctcggcga ggccccgttc ttccaggagc aggctcaaat ggaagagggc
    21241 gtccaggtga tccggatcga gataaagggc cttttgcagg ctcctgtcgg ccccctcggc
    21301 atcccccatg gcccactgaa cgattcccag gatgaaatgg gcttcgcagc aggtgacgtc
    21361 cagttccagg cattcgaggc aacgctctcc ggcttcccgc agcatccccg catcggccaa
    21421 acctcgcgcc gactcgagga gggcttccgt ccgccgcttc cgatcttccg aacccgtggc
    21481 ctcgatcagc gaggggagtc caagggattc ccggcctcct ggtgccccca tgggcgtgcc
    21541 ggtaccctga atccggtcat cggcggaaga acggggggat ctcgttgagg cgcagggccc
    21601 ctcggcaccg ggccggtccc ccggaacccg ggaggggaac cctttccgcc cctcgttccc
    21661 gggctgttcc ccggtgccgc aacggggaga caaggggcgg tgagcttcca ccggcgccga
    21721 ctccggtttc aggcaggcgt aggaccgagg gtggtccacc gggatgtagc cggggaaaaa
    21781 gatgtgggag ggttcggcat aaccgagaaa gaggagtccc ccacaggcaa gaagaccgtc
    21841 gatggtctcc gtggcccggg cccgggcctg gtcgctaaaa tagatgagca ggtttcggaa
    21901 aaagacaatg tcgtacgggc cgttcaccgc caatccgccg ggatcgagga ggtttccccg
    21961 gataaaccgg acggccccgc gcacccgggc gggcagccgg tagctccggc cgcttttttc
    22021 gaaatacttc cggcgcagat cctcgtcctg ctcgcggaag caggaaggac cgaagatccc
    22081 tctttccgct ttctccaagg ccttcctgct gatttccacc gcgtcgatgt ggaattggct
    22141 tccttcgagc ccggcttcca tcagggtcat ggcgacggaa tagggctctt ctcccgtcga
    22201 gcagggaagg gaaagaaccc gccaggggcg gcctccaccc ggggcaggga acctctttcg
    22261 gcggatgtat tccctgagaa acacgaaggg ttcgaaatct cggaaaaacc aggtttccgg
    22321 cacgaggagc gcctcgagga gctcatcccg ctcctcttcg ttctccgtca ccagggccag
    22381 gtaagcctcc tcgtcgggaa tcccgcaaat cttcattcgt tcccggacgt ggcgttggag
    22441 agccctgaac ggaacgcacc ggggatcgta gcccatttgg tcgcaaagaa accggtcgat
    22501 ggcctggggc cgcatggtca agccgatgcc tccgcgggct ggaacaagag ctcccgaacg
    22561 tcgggggaaa ggagctgccc gatctcgatg cgctggacga tcccccggga gtcccgggtg
    22621 atttctccga gatagggagc atccttgaca tgaatgccgg gggaggagaa tcccctttca
    22681 tccaccttga cggtttccgt cacctgttcc gcaacgaggc caaggaaccg gttttcgccc
    22741 gcctcgtcgg gatagcgtac caggaggatc cgtgtgctga ggaagccgtg ggattcccgg
    22801 gttcccgcca gtcgagccag gtcgatggcc gggacgatct tgccgcggta ctgaaacagg
    22861 ccgcacacat attcgggggc ccgcggcagt ttcctaaatt ccaccaaggg aaggatctca
    22921 acgacttccc ggctgtcaag accgaaacgg tccccaccga tggagaaaac gaccagaagc
    22981 atggctatgc cgccaccttg aaccgggaga cctcgtcctg gaggccccgg accgcttcgt
    23041 tgagctgctc cgcggcacgg ttgaattccc tcaccacctg ccgggtttga tccgcccctt
    23101 cgctcagcct caccatcgcc atgctgatct cttccgcccc cgtgctgtgg gcggccatgg
    23161 actggttggc ggccccgatg cggggaatca gcgcctggac ctgccggatc accgtggcca
    23221 gctgaccgct gatcctctgg acatcccgga ctccctggtg tacctgctgg atgaacttgt
    23281 ccgcctccat gacccccgac gaaaccgccg attgcatgtc tttcaccatt tgttcgatgt
    23341 ccagggtggc caccgcggcc tggtcggcca acctgcgaat ctcccgggcc accacggaaa
    23401 aacccaggcc gtattcgccg gccttctcgg cctcgatggc ggcattgaga gacaacaggt
    23461 tcgtctggtc cgccaccttg gtgatggtgg tcacgatgct gccgatgttg ctggtcttgt
    23521 cgctgatggc ctcgagcttt cccgcaatgg aggtggtggc gtccagaagg tagcgcatgg
    23581 tggattccat cccttgcagg cccccgtggc cttcttcggc cagggtggcg gtttccgaag
    23641 ccacggtgcc cacctcgttc atggtgcgcg ccagttcctg agaaccggcc gagatctcct
    23701 tggacgtggc gctgacctgg ttgatggagg ccgcctgctg cacggcggtg gcctcgagct
    23761 cgcgggccga ggacgaaatc tccgtggcgg agctcaccac ctggattccc gagtttctca
    23821 cctgaacaat gagagagtgc aaagaacggg tcatgtggcc gaataccgaa gaaagatggg
    23881 caatttcgtc ctttttcacc cgcagctgtc ccgaggcgtc ggtctcggta ccgaacgagc
    23941 tcaggtcgtc ggccgccccc tgcaggtccc cctgggcgat cttttccgcc gatcccgtga
    24001 tcctttgaat gggacccgtg atggagcgca cgatcatgac gcccacaaac aacaccaccg
    24061 aggccgcaac caggaaggcg acgacgttgg ctcccaggag catgttcccg aagcggacga
    24121 tccgtgcatt gttggtcgct atgatctgct ccttggcacc ggtaaccttg gcgcgtatct
    24181 gatccaggtc cgtttgcgcc atgatccggt cggtggaaag ttcccacaac ttgttgacct
    24241 gcccgggaag agagtcgatg gtcttggtca acgcttcggt gacgaagtcc gaatagtccc
    24301 ggtccacgta caccagggga atggccgtga cgctcttgcg ctccctttcg agttccatga
    24361 cgaccttctg ggaacgggcg aggtaactgc tcacgtcgct cttttggaac aattccacgt
    24421 agtagatcaa gaacatctgg ttggtaagaa agctgatttg ccgggcgtcg tccaagaggg
    24481 acttgagaga tgccgccgcc tgctgtcctt gagctgcctt ggcgtcggct tccttgctca
    24541 atttctgaac gaccgcttcg gtcttcttat tgaaccccag gagcgtctcg tggaactcga
    24601 cctcgaaagc actgagcttc ttcaccgcct ggcccaggtg atcgaaggcc tcccgatatt
    24661 tctcaaggag gcccttgaac agggccacgt cccccgtttc cacggaagtc ccgccctcga
    24721 gggccgacag cccctcctcg gccctcttca aggaagccag ggccgcctcg aaggacgtgt
    24781 ccttgcgcga atgaatgaag ttcctctcct cgatgatccc gttgagcagg ttctcactca
    24841 ccgcgttgag ctgacgaatg ccttcgtcga agttcctcat gacgcggttc aagaaatagt
    24901 tgatgccgaa ctggaccccg atcagcatga tgcccaagga gacaagaagg atcagtttcg
    24961 aggatatctt cattgcgggt ggcccttctt tagggacaaa agatcagaaa cagccgtcca
    25021 atgcaggcgg ggaaagaaac gcaattttct acccgatcct atctctacga tactcccctt
    25081 cccctgtaaa ggaggatctc agtttgttcg aggccgcccc gggaaggggc gtgcgccggg
    25141 accccaaggg aacgggaagg atgagatctc gatccccaat agttggtgat tatgcggaat
    25201 tgaatggcgt gcggcatcat gtggggccgg ctatgccgct cccacatgaa tcgggccgga
    25261 agccgtcttt cggcagttgc cgctgaccga cctgtctgca actacagagc cgcaggaacc
    25321 agggttccga tttgaaggca tgagatttga acccaaagcg gtgtccaaag cagggacttt
    25381 tcggggggcg atcttaaagc cccacccttg gggtggggct tcttattccg accagctcgg
    25441 atcaagacgt cggttcgagt cgctccggct cgacgggtcc tgaaagagcc cgtccccggg
    25501 cgggtcattc gggcgggtca ttcgagggga tcgttttcca ccaggttcca gattccacaa
    25561 gggcaagccc cggcgcagaa gccgcagccg atgcaacggt ccgggtctac ccgcatctcg
    25621 aagcctccgt ccccctccca tctcgagatg gcgctctcgg gacagatggc cgtacagatg
    25681 ccgcagtccc ggcagttgcc gcaggaagaa cactgggagg cgcactgttg cacgccttcc
    25741 atgtgaagca cccgggggtc gtagtactcg agctttaccc tcgaaagatc gatctttccc
    25801 ggggtccgga cgctgggctg cttcccggat aacaggtccg agatgacccg ggccaccctg
    25861 cgaccggctc cgatggcgtc cgtcaacaag cccagcttca cggcgtcccc cacggcgaag
    25921 atgcgggaat ccgtcgtctg gttgagctca ttgacccgga tgaagcctcg ttcgacggcg
    25981 acgctttccg ggagaaaatc gagatcgggc atgtctccca cggagacgat caccgtgtcg
    26041 gccggaatca cctctcccga ggtcagttcc accccttcct cggtcaccgc ccgggtaaag
    26101 cagggccatc ggaaccgggc tcccgcctcc tcggcggctt tccgctcctt accgaaggac
    26161 agaggttcct ggatgtcgat gagggtgata tcccgggcgc ccaggcgatg ggcttcggtg
    26221 gccgcgtcgc agcccacgtt cccggccccg atgaccacca cccggtcccc cacccggact
    26281 cggtccgtct tgctttgctc gaggaaggtc agggcgggga tgagccgctc ttttcccgga
    26341 atgggcagga ctcgcggttt gttggctccc acggcgacga ccacaaaatc gtaatcggct
    26401 cggatggctt ccagatcctc gcgggtgagg cgctgctgca agtgaacatg gggaatgagc
    26461 tgcctgaccc ggtccagctc ggcctcgacc acctccgggg gaattcggga ctccgggatt
    26521 tggaaggcga tctttccgcc cagggatttg cgctggtcgt acacgacggc ttcgtggccg
    26581 tggaggcgca actgccaggc cacggagatt cccgccgggc cgcctcccag gacggcaatt
    26641 cgttttcctg aaaggggagg caaggtcggc aactgggcct tgatgctcgc tcttcccagc
    26701 tggctgatat ccacgggagc caggttcatg gctccgcggg tgcatccctg catgcaaagg
    26761 ttgggacaca ggtagccgca gacggtggcc gggaaaggag tgtaggccag ggccaggttt
    26821 acggcctcgt ccacccgacc ttcccgcacc aggcgccatc tctcgtggac gggaattccc
    26881 gtggggcagc ttgcctcaca aggggctgca tacttgcggt tctcccacac gggcacgtat
    26941 cgacgcaggt tcccccgagt gatgagggga atgggacttc ggtcataggt caccaggtcc
    27001 ccgatgaggc cccccggtcc cagttcccgg tcccaggatt cccggcgaaa ttcgctcatg
    27061 gagcggcgat ccttggcgta cttgtcgtgg gggcctcgag cgacgagcaa ctgccattgc
    27121 tcgcgcaccg ccagctcctg gagcaaatcc ccatggcgga tccgatccag aaagatctcg
    27181 agatttcggt agagccaatc ccagtccgca tcggagatgg gagacaggag ggcgtcggcc
    27241 tggctgtacc ccttgtgagg gcctcgaaaa tagatcctcc ccccaaccat tcccacgcac
    27301 ggccggtatc ctaggacgtt gtccgggtct tgggggcggt agccgcacac caccgcgatg
    27361 ccccccgcca tgaactccgc gaaatagtcc cccaccgacc ccaacaccca gagctccggg
    27421 ggatcgaacc gggggttgtg cttggtcatg gtcatgcccc gggccccgat gtttccggcc
    27481 acccggacct ttccctgggc catggcgttg gccgccccgt tagcggcgtt cccgtggacc
    27541 gtgatttcgg ctccggcatt gagccatccc acgtcgtccg aggtgggtcc catgatctcc
    27601 actcgggtat tggggaatcc catggaaccc gttcgctgtc ccgaagaccc caccacgcgc
    27661 acccgaatgg gatcgtctcc cgctttccac agccgcccgc cgatgccgtg atgcccgtag
    27721 gcttccactt cgatattccg cacaccgtcg gccacggcct gttgaatccg ttcctccaaa
    27781 atgcgcgact cgatgcggcg tccgttttcc aggccggaaa tccggtagta ttgctgttcg
    27841 tccatcccaa aatctccgtg atccatcata cgacgtactg gatttgcagt cgttgcgcgg
    27901 cgtcggcgtc gttgattccc agggcgtccg acatcccgat gggcagggag gtggagcgtc
    27961 ccaggggcgc cacgatcttc ttcagttccg tgtcaaagct caggaacacg tccaccaccc
    28021 gttcggcaac cttctcggga tccaggcgac gataaagccg ggggtcctgg gaggtgatgc
    28081 ccttggggca aagcccgatg ttgcagatgt tgcagcgatc ggattcggag cccaggcagc
    28141 ctgcggccgc ctgcatcatg tattttcccg tctgcactcc gctggctccc aacatgatca
    28201 gggctgcggc gttggccgcc aggcttccgt actttccgat gcctcccccg gcaaagaggg
    28261 ggatctcgtt ttgcttgccc accctcacca ggttcaggta gcagtcccgg aggttggcgg
    28321 cgatggggtg tcccatgtgg ttcatggaga cgttgtaggc cgccccggtg cccccgtcct
    28381 cgccgtcgat ggcgagcccg gccgcgtagg ggtttcgggt caggttgttg aggacggcca
    28441 gggccgtaga ggtccccgaa atcttcggat agacgggcac acgaaatccc caggccatgt
    28501 acatggactg gatcatcttg gccacggctt cctcgatgga atacttggtc tggtgggtgg
    28561 ggggactggg caggctcacc ccatggggaa ctcctcgaat ggcggcaatc agcttgttga
    28621 ccttgtacca catgagcagg ccgccgtccc ccggcttggc gccctggccg tacttgatct
    28681 cgacggcgca ggggtcttct ttcatctcgg gcagggcgtg aatgatttca tcccacccga
    28741 agtagccgct ggcgatctgc aggatgacgt acttgaggaa ccgggacctg agaagccggg
    28801 gaggacatcc tccttccccc gtggacatgc gaacgggcat gcccagttcc tcgttgagat
    28861 aggccactcc catctggagc ccctcccaca tgttgggcga cagggccccg aaggacatgg
    28921 ctccgatgat cagggggtag atttcacgaa ccggggggat ccagccctgt tcccggtaga
    28981 ccttgaggcc ttcctcgggg ggcagtaccc ttcccaaaag ggtgcgcagc tcgaactcgt
    29041 ggcggccggc atccagggcc gggtcggtga gcatggagat gcggatgaac ttgatccggt
    29101 ccagcagccc ccccggcggg gaatccggca cgtttcttcg accgccccgg gaacggggct
    29161 gtcccccgcg gttgatgtga aaccgcagct tgtcggcttc gtcactgcgg taggggatga
    29221 tgcagtcgtt gggacacacc aggttgcagg aggcgcaccc cacgcaggca taggccggat
    29281 cggttctctg ccggatgccg tggaacaccg agtacacgtt ggagggcggt ccctgggttt
    29341 ccccgccgtc cagggagccc agccggatgt ctcttttccg gtagaccccc agctcgatgg
    29401 cgtgcaccgg acatacggcc gtgcaccggc cgcagagggt gcaccggtct ctttcccaac
    29461 ggatctgcca gggcaggtcc ttgacgctca gtgtagaggg ggtaatgggg ctgtttggga
    29521 gcatatatgc acctcctggc gatccggccg cacgaacacg gtgtccagat gcatgggttg
    29581 aaagtcctgg gtttcgtccc ggtccgggat gacggcgtcc aaaccgcaca tttccgacga
    29641 aaaggcatac acccccggtt tgcctcccac cactcccggt cgaagcttct tgcggtcctg
    29701 gaccatgaaa agggtcttgt ccggaagaca cccgatgacg caattggggc cgtcgatgat
    29761 cagccttcgg caggagttct tcatctgctt gaggaacccc ccgtcggggt ggacggccag
    29821 gtccgcatcc tggaggggtg tgatcaggtg cttgtaggct tcgattccca ggcccagccg
    29881 gttcagggaa tagtggagaa tgtgggtgaa cacctcggag tcggaattgt agccgatgta
    29941 cccgggaaac ccccgggaca tgaggaattc cttgatggga acgaaggccg tgttctcgcc
    30001 gttggtcatg gtggccaccc cctggatgaa gaacggatgg caggcgtaca ggttgatggc
    30061 gtaattggtg ttctggcgcc cctgggcgag gatgacccgg gccaggatgt ccttgcgccc
    30121 cagggagagg tagtccgcca cccggagcgg gtctccgatc tccttgatca tgatgacgtc
    30181 gggccagaag ctgtagacaa tcatgtcctc cttttcccgg cccatttccc ggagctcaag
    30241 gcggacgagc atgaggcggt gccggatttc ctgctcgctc aacgtttccc aggattccgg
    30301 gtattcatag gcgcggatga ggtaaatatc gcgcttggga acccccgggg gaggggtttt
    30361 gggcaccttg atggaaatct tgtacttggt catgaacccc atgttcatca tgaactggtc
    30421 caggcgcttc aggccttcgt tggtgaagat gcccgacagg atgggggcgc ctttgagctc
    30481 ctcgaaatgc cccccgaggt cggtgaggaa gagaccgacc cccgagccgt cgtggccctc
    30541 ccgcatgacg tccagggcct ggacggccac catgggggac aggggggttt cactggtcat
    30601 ggcaaacaaa cgacacatgg gcttttatct ccttactggg acacggttcc tgggaacggg
    30661 ttttcccggc gaaaagcttc tggtattgta aagcaaacaa gaccgtttgg gaaatgggtc
    30721 ccggttcact gtaagcaagg cttatgccag ggcctccttc ggcattggga ctaacggccc
    30781 ggaggccggg acccgggaag ggagggaccc ccatgctttg gctcagaaag cggcgtcgca
    30841 accgaatccg ttcccgaccc tttcccgccg cctgggtcca tttcctcgag aaaaacgtcc
    30901 cctactaccg gctccttccc gaggaagaca aaaaggaact tcagggtcat gtcctggttt
    30961 ttcttgaaga aaagcacttc gagggatgcg gcgggctgga actgaccgac gagattcggg
    31021 tgactgtggc ggcccaggca tgcatactct tgcttcaccg ggaggcggga tattacccga
    31081 ggctggattc cattctcctc taccccggcg cctatgttgc ccgggggcac gaggagctgt
    31141 ggccggggca gttggtggag acggaccagg tgcggctggg cgagtcctgg aaagcgggcc
    31201 tcctggttct ctcctgggat catgtgcgca gatcggcctc gagtcccatg gaatcccaca
    31261 acatcgtcct ccatgaattc gcccaccagc tggacctcga agacggtctg cccaacgggg
    31321 ccccgctgct ccccgaccga tcctcggtga ggacctgggc cagggtcctg ggtgaggact
    31381 accgcaggct tcgaagggac gtgtggcgag gacgggaaac cctcctggat caatacgggg
    31441 caacggatcc ggccgagttc ttcgccgtgg tcaccgaatg cttcttccaa aaacccctcg
    31501 agatgaaggc ccaccatcca cggctctacg aggtcttgaa gtccttctat cgacaggacc
    31561 cggccgaatt ggtcgaaagg caggaagccc tcagttgagc aggctcgccc ggtggaggat
    31621 ctccggcacg gccttctccc ttcccgccgc caccaggtgc acgccgtggc agatgccttc
    31681 ctccttgagg gtccgcacca tgcgggccgc aatctcgatg cccttctttg cctgatcctt
    31741 cttgggaacc tgttcgagtt cggaaatgag gtccccggga acggccatgc ccggaacgtt
    31801 gcggttcatg aatctcgcca ccccggcgct caggagcggc gcaattcccg ccaggatttt
    31861 cacccggtag ttccgggccc ggtccataaa ccgtcggaag gaatccatgt cataaatcgg
    31921 ctgggtgagc acaaactcgg ctcctgcccg aatcctcgcc tcgaggtccc tcagttggtc
    31981 ctcgagggac ctggttccca ggaccaggac cgcaccggca caaaacttgg gggccttctt
    32041 gagggcgtgg cctcccaggt ccctgccgga ctccagggtt cgaatggccc ggaggatctg
    32101 aagggaatcg ggatcctcgg gcccaaagga aggtcggtgc cctcccgagc gcagggaagt
    32161 cacggcggcg ctaagcaccg tgtcgatgcc cagcaccgcg gcactgagga ggttcgactg
    32221 gaggaaggat gtgttcccgt ccccggcgga cacctggagg atgggcacgc cccccatctc
    32281 cttgatgagg tggcagacga ccagggaggg caggtggacg ccgttgtcgg gctcatccgt
    32341 catcttcagg ccgtgtacct tgtccttgag gtatccgatg taatgcttcg tgtcttcgat
    32401 atcgattcct ttgggaacgc cgatttctgc ggtcacgagg aaatcgccgg aatggagtgc
    32461 gtctttgaaa gaggccgtca tggtgttctc cttctggatt gcgccccggg gggcatccgc
    32521 ggccaatggt tcccggggga acccctcgga ttgggcggaa gagttcccgg tgcaaaaggt
    32581 gcgcctgcga tggaggcccc cgggggaggc aagccgtcct tggatttccc gcccccgagt
    32641 cgacccgggg caggggtcaa ggttgtgaac ttgaggagcg acgcgaggct tcctcgcccc
    32701 cgccccaatc ctctctacag cctcgacggg cggaacccga tcctccgggc cggggaatcg
    32761 gaattttcgc cgccgtcgaa atccgggttc gtccaacccg gtcagacgag aatcccggcc
    32821 cgtcatggat tccggaaaac tgcgctccct gccggattcc ccggggggcc gggcttcgcg
    32881 gaggggaaca atcggtcaag aagcatccgg gggggaccct cgggccacca cccggtcctc
    32941 cccaaggcgg aaaagggtaa gagattccct tcaaaaatac aaaaatgttt cgtggattga
    33001 caaggaaatt cctatacatt tttggggaaa aatgagaaaa tgattccgta agatactgat
    33061 atacaagcag tacttaagtt aacgcgtctt ctgtaacctc tactaacagc cgataagtac
    33121 ctctaagaac gatatcacat ttttgtaccg ttactttcaa acgagagggg aattaaacaa
    33181 aaatgtaccc gggtcggggg ggtcggggcg gggatgggaa agattgttca tttggggaag
    33241 gcggccgggg gtgaaaaaaa ccgccggcac gcaatttgtc taatgaagaa ggaggaaccc
    33301 aaaccaagca caagaaagga gccaacccat gatcgacgtt tccatcaaat acaacgtgac
    33361 cggcggcagc cagccctgcg ccctgtgcgg agcgctgacg gaatccaaac acggcccgga
    33421 cctctttctg gaaggcacca tgcaggtcat ctgtccggag tgcggccagg accacgcacc
    33481 ccacctggcc cgcttgctcg agttgggcca acaggcccag tgctacaccc aagcctgcgc
    33541 ccgctgaggg ggctctcgaa agggcagaga atcttctccg gccgaaaaag gaggttttcc
    33601 ccggggtttc cccggggatc gacagcgcct ggaacccggc ctgatttctg ctatagtgga
    33661 aaccgatggg aagccccatg gggccgtggg cctttgtccc atcgagggac ccgccggcat
    33721 ccgccacaat cacttggttg gcttgagccc ggcggcatcg gccgggtctt tgtcccgggt
    33781 ttcgcagacc ggggagggcg caggacgggc ggcgaactcc cgggggctga tccccgacgc
    33841 cggccggccc gatcccggca gaatcattcc atgtgttgga ggccaaagga gaggacggta
    33901 tgtcggatcg acctcgagtc gtctcggggg tgcatcagct ggatgccctg ctcggggggc
    33961 ttttcatcgg agacaacgtc atctggcacg atgactccgg aagcctggct tcggtgttct
    34021 gcctcaattt catccgggcg tccctggatc agaaccggcc cctcatctac gtcagtttcg
    34081 atcgctcccc gcgcaacctc ctcgagaagc tcggacccct tgccgatcat ccgtccctga
    34141 ccatcctgga ttgtttcacc agcggcaagg gggcgagctc ccccgttttt ctgcgcttct
    34201 acgacgagcc gggcacggac cgggcctgtg agattgtccg ggtggaggag ccccgcaaga
    34261 tcgatcacgt gatggaggtg ctctacgggg tccacgcggc cctcgagggc tatgtgtgct
    34321 ttgtgttcga gagcattacg gggatgcagg aggtttgggg aggggaggat cacatcctga
    34381 atttttactc ccattcctgt ccccgcctct acgaactcaa caccgtggcc tactggatca
    34441 tggaaaagaa ggcccactct ccccggctcc gcgcccaaat cagccagatc gcccaggtgg
    34501 tcatcgacct gggaatcaag agaggcacga cgtccttgac catcctcaag gcggaggatc
    34561 ggaacccggc cgtttttcac aaaccccaca actactggac ccgggacctg accatcgcct
    34621 tcgacgagga gaggcgaatt cccgggggag tggacctggg agtgcgtttg aaggagctgc
    34681 gaagcaagcg gggattgtcc cagacggacc tggcccgcct ggtgggggtg acgcccagca
    34741 ccatatccca ggtggagagc aacctcatct acccttccct ccccgccctg ctcaagatgg
    34801 ccgaggtgct ggccgtggac gtgagctcgt tttttcagga aaagccctcc gccgggcacc
    34861 gccttgtatt caccgccgcc gatgccgtcg aggtgaagct gccccagatc cccgaaggtt
    34921 ttgcctacgc ccggctcctg acccccccgg acctggagcc ccgggcggaa ccgtttctca
    34981 tcgaggttcc tcccaacacc accattccct ctcacttctt tttccacaag ggacacgaac
    35041 tgggatacct ggtctcgggc aagctccagg tgaagttcgg acaggcctcc tacacccttc
    35101 ggggcgggga cgtgttgtac ctggattccc agctcccgga tcagtggaag aacccgggaa
    35161 gctctcctgc tcgactgctg ctgatcaaga ttcggtgaaa gagaaaacgg gaagagggaa
    35221 aggcaaggtc gggtccccgg tcccgcccct tttcccgggc ccccgaactg cgtacgagaa
    35281 ccggggaaag ggaaaggacc ggggaagtgt ggtggtcggg gagcgggggc ttcccgtttt
    35341 ggcctagctg ttcagaatgg cttcggcttc ttcccgatac gcgtccatca cgtacggaat
    35401 ccccgtcacc cgggtgcact cttcggttag ggacatgagc tccctgcggc tgatggagga
    35461 caggttgaag cagcgggacc cggccatgag ctgctgcaat cccacccgga tcttgtccga
    35521 gtaactataa atcccgatgg cgcccaaggg aatgttcttg atctcggcat cgcccaccag
    35581 gtctttgacg gcttcgtagc tcacgaaaat ctcttcggcg gtgtttccgt actggcttac
    35641 cgtcttgggc aaatcgtttt tctgtatcca ttcggcgatg tttttgccca ccattccggg
    35701 gatcatcaag gcacgaccca tacagatggc cttcacgaag ggggacccca gggccagggc
    35761 cttgaagacg ccgtcctcgc tggagaatcc gcccgcgaag gcgatgtcgg ggaccctctc
    35821 tccccgggcg gtgagttttt ggcagaaatc gtatgccgcc gaatgaatgt agaggctggg
    35881 aattccccac tcttccatca ttcgccaggg actcatgccg gttccccccg gtgcgccgtc
    35941 aatggtcaga agatcgattt gggccttgga gccccacttg atggccatgg ccagttcccg
    36001 aaggctgtag gccccggtct tgagggtgat tcgcttgaag cccagttttc gcagccgagc
    36061 cacttccgcc aggaacccct cttcgctcac gaaccccagg cggctgtggc gctcgaattc
    36121 cttgatggct ccttccttat aggccgcctg ggccacgggg ctggacggat cgggggtgat
    36181 gatgtagcct cgcttttgga gctcgagggc ccgttccagg ctgtggacct tgatctctcc
    36241 cccgatgcac ttggcgccct gaccccattt gagctcgatg gtttggaggc cgtgcttgtc
    36301 caccacgtac tcggcaacgc ccaaccgggt atcctcgacg ttcatctgga ccaggagctc
    36361 tccatatcct cgatggtagc gcttgtagat ctcgatgcgg cgatccatct ccggggactt
    36421 ggtgatcttg tggtcggcat tgagctcgag gccgggatcg atgccgcaca cattctcgcc
    36481 gcacaccagg gtgacgccgc tgatggccgc ccctacggcg aagtgttccc aattcttgcg
    36541 ggcaatctcc gtggaaccca gggcaccggt gaaaaccggc atgcgcatct tgactttgat
    36601 gtcccagccg tactccgttt ccgtgctcac cgccggaaag accgccgtat ccgagttggc
    36661 ctcgagtccg ccgggaagac cggcgccgcc cagggcgtac ccctggatgt tgaggtggga
    36721 atagtccacg gggtagttct tgtcggcgcc ggcggtgatc tgcccgaagg gacccgggta
    36781 gatcacttcg cggccgcgaa acgtggctcg gaagacctcg caattccccc ggcatccgtc
    36841 gatgcagcgg gagcagagac cgctcatggg gaccacgctt ttcgaacggt tgaaggtcaa
    36901 ggttgcgtca ttggaattgg gggtgctcaa attcatggtt tgtttctccc tctcgttctg
    36961 atcgttggct tttggagaat aaaaaaatcc acaccccctc gacgggggga tgcggactgc
    37021 ttcgtccgaa gtttcggcca ctccgtcgta gcctgcaaaa tagacgagcc cggaaatgca
    37081 aaaaggcgtc tttcccctcg gggggaacgg acgcctttgt cctgctcgac cgcgctaatc
    37141 ccgcactccg ttgtgcgagg ctgccgcgct ttcataccct ctaacaaatt cacggtagct
    37201 ttagcgttac ttatagagtt tgtcaaggag attgtttggg cagggggtgg gagtccggga
    37261 gccgaagcac gtgcgtgccg ggttgagcgt ttccgaacgc ggcagcccgt cgtgcccgca
    37321 aaggggcatc cggcaggtac ggaagctctt tccggactcc cgcttgcgcg ggagggacga
    37381 aacgggattc gcaacagccg aatcgatcaa tccggatgcg actcgtcttc gaggttcaaa
    37441 ccagcgaggg gcagaaggcc cccagggctt tccgcagggt ctttcgcatg cgaagcaggt
    37501 cctgccagtt ctcgagggat tcttctgcgt ctttgacggg ccacaatccc agggctctca
    37561 tttcgtgcaa ctgccggggg gaaatcttcg aaccggctgc attgctgtaa agacgcaggc
    37621 ggtgttcaag ggccctcagg gccttgaagg cgaccatcac ctcccgacac tcggactcac
    37681 ccgatccgat gctcgtcagc acgcttttca agcaggaccg cacggacccg cccgcgggga
    37741 aaaccttctc atggccccgg gccaggtgca ggccctgcac caggaattcc acgtcggcca
    37801 atcctccgtt tcccagcttc aaatccagga gatccgggtg ctccccggcc ctttcctcct
    37861 gcatccgctg tctcaggtgg caaaggcggg cccagacccg ggactcatcc cgcttcccgt
    37921 aacaaatccg ggaggccgtc ccctcgatgt cgcgccccac ggacgggttc ccggcaatgg
    37981 ttcgaagtcg aaggagggcc tggatttccc acaggtccgc gtcccgggaa taatactcga
    38041 tccaggacct ttcggtgacg atgaggggcc cgtagtttcc cgtgggcctc aggcgaacgt
    38101 ccacggcgta gccgggtcct tcctggaggg gagtgctcag catgcgcatg aaccgttgca
    38161 ccagtcgaag aacggttccg ggaatttcat cgtccgattc gccgggggcg ggacgataca
    38221 caaacaccag gtccaggtcc gaaaggtagc tcatttcccg gctgcccagt ttcccgagtc
    38281 ccaggacggc caggggaagg tctcggggca ggcccaggtt gcggcagacc ccgccgtagg
    38341 tatggcgtac cacaaaatcc gccaggccgc tgagctccaa ttccagttcg aggtcgctca
    38401 aacgcccggc caggtcttcc cgggcaatct ggaccgttcg ttcgttcttc agccgccgga
    38461 tccattccat ttcttgttcg tagtccaggc ctcgatccag gactcgggcg gcgttgcgtt
    38521 cccagacggg gcccggggga aaggccccgt gggtggtggc catcccctcc accaggctgg
    38581 ggttgtgcgc cagcaattcc cccagcagct ccgagcgacc caggcccgag aagaactcct
    38641 ccatccaggg agcgaaggca tggaagactt ttgtcaaccc cggtcttctg cgaacatggg
    38701 ccaggtagcg ctcgatgcgc agaaggatct tttcccggat ttcggtttcc cggagggcgc
    38761 cgtacgggcg caggaagcga accagggcgt tgtgcaggtc cgcggggaag tcccggaggt
    38821 gagcctcgag gcggtctcgt gcctcccggg gataggctgc cgggaaatca cggctcccct
    38881 ccccgccgtc cgtgatcgac tcccgggggt ggggatcccg ggaacggaaa aggtccagga
    38941 agtgctcatg gaccctgcga caatgctcct gcaaccgatc gaacaaatcc cggacgtttt
    39001 ctccgatccc ggccgctcgg cagatgcgca gcagggattc gggcgaccgg gggatcctgg
    39061 aagaatgccg gttctggtcc agctggatga aatgctcgat tcgtcgcaga aaggagtagg
    39121 actgggccag ggattccgcc acggaatccg agagaaggcc aaggtcgcgc aaggcttgca
    39181 gggagcgcaa agagttgggc tcttccagtt ccgggtgcct gcctccgtag atgagctgca
    39241 gggactgaac caggaattcc acctcccgga ttcccccgac tcccagcttc acatcgaagt
    39301 tttcccagtc ggaccgggga cggggggctt cggaaagaat gcgatcccgc atggcccgga
    39361 tctcgtcgag ggcttgaaag tccaggaagc ggcggaaaac gaaggggcgc acttcttgga
    39421 ggaaggcggt tcccagggaa cggtttcccg ccacgggccg ggccttgagc agcatctggc
    39481 gctcccacgg tcggccgtgc tggaggtagt ggtcggcggc gccggaaaga gaggggacca
    39541 ggggaccgtc ctttcccccg ggcctcagcc ggaggtccgt ttcgaagacc ctgtccccgt
    39601 ccacgtggtc ggagaggagc tttcccagcc actggaaaag acgctgaacg agaaccggga
    39661 tggagtccgg aagggcttct tccatcccct cgtgggaata aaggtaaaga aggtccacgt
    39721 ccgaaacata attgagctcg tggcctccca gctttcccaa tcccaggacc accagtccca
    39781 ggcggcctcg ccaggtcgcc catgcccggc tttcttcctt gtccgcccac cattggggag
    39841 actggtccag gacgtccagg cccacctgga ggcaaaccgt cgccaggtcg ctcaactgcc
    39901 ccgtcgtctc ggccaggttc gccattccca ggaggtccct ggccccgatg cgcaggaaat
    39961 ggcgctgctt gaactcccgg aaggttcgca ggagcccctc gaaggacggg gcggaccgag
    40021 cggttcgctc gaggtcctgg aaaaggtcgg tgagcccgta gcggcggtgg aggttgcccg
    40081 gggaggagag ccattggcgg agctcgggac gggcgcgcag gagggtgccc aggtactggg
    40141 aacagagggg aatgcgggag agatccggca ctggttttcc ttgcggtgga ggcgcccgca
    40201 tcttgaaaac ggaaccgccg ttgaataatg gcgggggttg tactataccc gttggggatt
    40261 ggaaatttcg ttttcgtagg agcggcattt tgccgcgaaa ttgcatggtc gcggcaagat
    40321 gccgttccta cagcaaagag gaaaagcgcc ggaacgtaac ttacaaaccc aaacgagtat
    40381 aacatccgcc cgcgaactct tgcggggtcg gccgcaggtg gggggaacgt ttcccccgcc
    40441 ggcggaacgt tagcacaggg cacgggagca tgactaccgg caaatcgccc ggaggctgcg
    40501 gatcgtttcc gggcgaagca caggcaccgg attcaacaga aggcgacgca gatgaaggtt
    40561 gtgggcatca tcccggcccg gtatgcctcc acccggtttc cgggaaagcc cctggcggat
    40621 atcgggggca aacccatggt ccggcatgtt tgggaacgag cggcctcgag cggttccctg
    40681 gaccgggtgt gggtggccac ggacgaccgt cgaatcctcg agaccgttcg ggacttcggc
    40741 ggaaacgccc tgcttaccgg cgaccatgcc agcgggaccg accggctggc ggaggcggcc
    40801 cgtattctgg ggctcgaggc cgaggacgtg gtggtgaaca tccagggaga cgagcccctg
    40861 gtgcgggcgg aaatgttgga tcggctcgtg gcgggcctcg aagaagatcc ggccttcccc
    40921 atggcgaccc tggcccacca gaccgacgat gagagggaat accgggaccc caacgtggtg
    40981 aaggtggtcg tggatcagct gggaaaagcc ctgtattttt cccgctctcc cgttcccttt
    41041 cgccgggacg aacccgccgg gtctcccgtc ttcctgaaac acctgggttt ctatgcctac
    41101 cggcaatcct tcctccagac cttcactcga ctccacccgg gaaagctcga acgcctggag
    41161 aagctggagc agctccgggc catggaacac gggtacctca tccgggtcat cccgtccccc
    41221 tgggatacgc ggggcatcga cacccccgag gacctggaaa gactcctcga ggagctttcc
    41281 gggaacgcgt cgtctgcatc gggggagcgc ccgggtgaac ggcgcgacga aatgagttga
    41341 tgttcaggga tttcccgtta caattcccgg gcctgcggac ggccggggaa ggctcagggc
    41401 acggtttctg caactctcct gcggttcgtg gaaatcccaa tccccgcgga tcggacgggt
    41461 tggctttgta aggaatccac cacaagacac gaagatcggt caaaccccgg tcggcttcga
    41521 accttgcgga gtcaagggac ggggttttgt gcactcatcc ttatttctgc atcgaggaac
    41581 gggtatgacg gttccatgga aacgaaccag ggcggtcgtg gtcctggaag acggcacggt
    41641 cttccgggga tttgcctttg caggggccgg gcgggccctg ggggaagtgg tgttcaacac
    41701 gggcatgacg gggtaccagg aggtgctgac cgacccttcc tacaaagggc aaatcgtcgc
    41761 catgacctat cccctcacgg gcacctacgg catcaacgcc gcggacatgg aatcggaggc
    41821 cgtccaggtg gaagggttcg tggtcaagga ataccaggaa tttgccagca actggagaag
    41881 cgagaagacc cttgcgcggt ttctcgagga acacggaaag atcggcatcg aggggatcga
    41941 cacccgtgct ttgacccgcc acattcggca ggcgggggcc atgcgcggga tcctctccac
    42001 ggactcggac gacgttcccg agctcctcga tcaggtgagg gcctttccgg ggctccacgg
    42061 ggtcgacctg gttcggctgg tgacctgccg cactccctat ctttggattc gcggggaaaa
    42121 gatcgagcgg ccgtcccggg agtggtggcc cgggcatccg ggacatcgcg tagccgccct
    42181 ggattgcggg atcaagttca acattcttcg gaaactcgag gaagcgggat gccaggtcat
    42241 ggtgtttccc gcagacaccc cggcggaaac catgctctcc gtcaagcctg acgccgtctt
    42301 cttgtccaac ggcccgggag accccgcggt cctcgagtac gtcatccgca ccgttcgggg
    42361 attgctgggg caaaggcccg ttttcggcat ctgcctggga caccagctca tgggcctggc
    42421 cctggggggc caaaccttca aattgaagtt cggtcatcgc ggggccaacc agcccgtgaa
    42481 ggatttgagc accggaaagg tcgagatcac ctcccaaaat catgggtact gcgtggattt
    42541 gagctccatg agggaccttc ccgttcggct gacccacgtc aatctcaacg acgggaccct
    42601 cgagggaatg gagcatctcg aggtcccggc tttcagcgtt cagtaccatc ccgaggcttc
    42661 cccgggtccc cacgacgcct cttacctgtt ccgccgcttc gtggccatga tggaaagaaa
    42721 cgggccgaaa acttcctcgg ggctcgccga aaggaactgc gttcatgcct aagcgaacgg
    42781 acatcgggaa gattctcatc atcggctcgg gcccgatcgt catcagccag gcctgcgaat
    42841 tcgactactc ggggacccag gcctgcaagg ccctgcggga agaaggctac gagatcgtcc
    42901 tggtcaacag caatcccgca accatcatga ccgaccccga gacggcgcac cgcacctaca
    42961 tcgaacccgt cactcccgag gcggtggcca agatcatcga aagggagcga cccgacgccc
    43021 tgcttcccac tttgggcggg cagacggccc tgaacgtggc ggtgcgaacg gccgaaatgg
    43081 gggtcctgga ccgctatggg gtcgaaatga tcggcgcccg ggtggacgtg atccggaagg
    43141 ccgaagaccg ggaactcttt cgggacgcca tgaaaaagat cggcctccgg gttcccgcca
    43201 gcggcattgc ccgaaccatg gcggaggtgg aggccgtggc ccgggagatc gggtttccca
    43261 tcatcgttcg tccttcgttc accctgggag gcacgggagg aggagtcgcc tacaaccggg
    43321 aggaactgct ggagctggcc caggggggcc tcgatgccag cttcatccac acggtgatgc
    43381 tcgaacagtc ggtgctgggg tggaaggaat tcgagctcga agtgatgcgg gaccggatgg
    43441 acaacgtggt catcatctgt tccatcgaaa acctggatcc catgggggtg cacacgggag
    43501 actccattac cgtggccccg gcccagacct tgaccgatca ggaataccag cggatgaggg
    43561 acgcatccat cgccgttctt cgggagatcg gggtcgaaac ggggggatcc aacgtccagt
    43621 ttgccgtgaa tccccaagac ggggaactgg tggtcatcga aatgaatccc cgggtttccc
    43681 ggtcttccgc gttggcgtcc aaggccacgg ggtttcccat cgccaagatt gcggccaagc
    43741 tggccgtggg atattccctg gacgagctcg ccaacgacat cacccgggag acccgggcgt
    43801 ccttcgagcc caccatcgac tattgcgtgg tgaaggttcc ccggtttacc ttcgaaaagt
    43861 ttccgaaaac cgaagacttc ctcaccactt ccatgaaatc cgtgggagaa accatggcca
    43921 tcgggcggac cttcaaggag gccctccaaa aggccgtccg ctccctggaa atcggtcgtt
    43981 tcgggttccc ggacccgccc tcccggcccg accccgatta cgtcgaaggg ctgaagcaag
    44041 cgatgcggcg ccccaactcc cagcggctct tccaactggt ggaggccatg cggctggggg
    44101 tggaaatccc cgagatcttc gagctcacgt tcatcgatcc gtggtttctc caccatatcc
    44161 ggcagattgt cgacctcgag caggccgtcc ggtcctggga ttccccggca tccctcatgg
    44221 aggacgagga ttttctccga gcggccaagt cctgggggtt ttcggacgtg cgccttgggc
    44281 aacttaccgg aaacactgag gaaaccgttc ggcaggagcg aatccgcagg ggaatccggc
    44341 ccgtctacaa gctggtggac acctgtgccg ccgagttcga agcctacacc ccctactact
    44401 actcgaccta cgagacggaa aacgaagcca ggccgtcctc ggggcccaaa gtggtgatcc
    44461 tggggggagg ccccaaccga atcggccagg ggatcgaatt cgactattgc tgcgttcacg
    44521 cctccttcgc cctcaaggag gaaaacgtgg aatccgtcat ggtgaattcc aatcccgaga
    44581 cggtttccac cgactacgac acttcggaca agctctactt cgaacccctc acccgggaag
    44641 acgtgctgca catcatcgag gaggaaaacc cccgggggct catcgtgcag ttcggcgggc
    44701 agacgcccct gaacctggcg gtccctctcg agaaggcggg agccaggatc ctggggactt
    44761 cccccgacgc catcgacctg gccgaagatc gcaagcggtt tcagcagatg ctccggaagc
    44821 tgggtctgcg ccagcccaag aacgccaccg cctttacggt ggaggaggcc ttggcggcgg
    44881 ccggggatat cgggtacccg gtggtggtcc ggccgtccta cgtcctggga ggacgggcca
    44941 tggaaatcgt ctacgacgat gcgtccatgg agcagttcat gcacacggcg gtccatgttt
    45001 cccccggcca tcccattctc ctggaccagt tcctcgagga tgccgtcgaa ctggacgtgg
    45061 atgcgatcag cgacggggag atcaccgtgg tgggggggat catggaacac atcgaacagg
    45121 cggggattca ttcgggagac agcgcctgcg tgttgccgcc catcaccatc tcccgggaga
    45181 tgcaggagga aatccggagg cagacccggc tcatggcccg ggaactggga gtggtggggc
    45241 tcatgaacgt gcagtttgcc atccggaaca accggatctt cgtcctcgag gtgaaccccc
    45301 gggcttcccg gaccgtgccc ttcgtgagca aggccgtcgg gattcccctg gccaagctgg
    45361 ccaccaaggt tatgctgggt aagtcgctaa aagagctggg ttttgtgaaa gagatcgaac
    45421 cgcgtcacct ctccgtgaaa gaggccgttt ttcccttttc ccgattcccc gacgtggata
    45481 tcctgctggg accggaaatg aagtccaccg gggaagtcat gggtatcgct cccagtttcg
    45541 gcatggcctt cgccaaggcc caatccgccg cgggctatga tcttccccgg gaggggaccg
    45601 ttttcatcag tgtgcgggat gccgacaagc ccaaggtact tgacgtcgcg cgggaattcc
    45661 gccggttggg cttccggttg acggccacgg gcggcacggc ccgttttctc cgggatcggg
    45721 gactcgaagt caagacggtc tgcaagctcc gggaaggacg gcctcacgtg gtggaccgca
    45781 tcaaaaacgg ggagatccaa ctggtggtga acacgagccg gggcaagaag acttcgtcgg
    45841 actcttatga aattcggcgc acaaccttgc tctacaacat tccctacgcc acgaccatcg
    45901 ccggggcccg ggccatggcg gcggccgtcc aggaactgca gcggggggaa tgggatgtga
    45961 ggacgctcca ggaatatcac agcgaaatcc gaaaccatga atgagcctgc gtttgcaccg
    46021 ggacacggat tcccgggcgg gcgtcggacg gaccttgctg ccgggaatcc cagtcccgga
    46081 aggcttttgc cggaaagacc cgactgatga ccagtgattt cccaacccgc atgacccggt
    46141 gttccaccct ggttcccgta aggtaccgac ccgggaaacg ggaagaatgc gggatcttcg
    46201 gcgtgttcgg tcacccggac gcggcgaagc tcacctattt tgggctctat gccctccagc
    46261 atcgggggca ggagagtgcc gggattgccg tatccgacgg gtgcgaaatg tccgagcaca
    46321 agcacatggg attggtttcc gagggttttc gggaagagac cctccgggcc ctcaaggggg
    46381 acctggccgt cggccatgtg cgctactcga ccaccggctc ctcccttctg gccaacgccc
    46441 agcctttcgt ggtgtcccac gggggcgagt actacgccgt agcccacaac gggaacctgg
    46501 tgaacgccgt ggaactgcgg cgggatctcg agagcagggg ggccattttc cggtccacca
    46561 tggacacgga agtggtcatg cacctgctgg cgagaaacct caggctggga ttggaagagg
    46621 ccctggtgga aaccttgcgc cgggtgcagg gggcctactc gctggtcctg gccaccaggg
    46681 acaagctcat gggaattcgg gacccgagag ggtttcgccc cctctgcctg ggccggctga
    46741 acggcagcca tgtgctggcc tcggaaacct gcgccctgga cctcatcgag gcgacctatg
    46801 tgcgggacgt ggaaccgggc gaggtcctca tcatcgacaa gaacggcatg agctccctgt
    46861 acccgtctcc cgaggttcgg cccgcccact gcatcttcga attcatctat tttgcccggc
    46921 ccgacagcag catcttcaat caaaacgtgt atctgtggcg aaaacggctg gggcacctgc
    46981 tggcccggga atacgggtcc ctggggggcg acctggtgat gcccttcccg gactcgggca
    47041 actatgcggc cctgggcttt gccgaagcct cccgggtgcc cttcgagatg ggagtgatcc
    47101 gcaatcacta cgtgggtcgc accttcatcc agcccagtca ggccatgagg gatttcgggg
    47161 tccgggtgaa gctcaacccg gtgcgggacc tgctccaggg caagaaagtg gtcctggtgg
    47221 aggactccat catccggggc accaccactc gaacaaggat caacgccctt cgccaggtgg
    47281 gagcaagaga ggtgcacatg atggtgagct gcccgcctca tcgattcccg tgcccctatg
    47341 ggatcgattt ttccaccaag ggggagctca tcgccgcctc ccactcggtg gaggaaattc
    47401 gccggttcat cgggctggat tccttgaatt atctcagcct cgaggggctc ctcgaggggg
    47461 gaaacgccgt cctgaacgat cacccgttct gcctggcgtg ctttacgggg gattacgcgg
    47521 tgcctttcaa ggagcaggtc cgcaaggact gtttcgaaag gcaggaacac cgctcataag
    47581 ggggaacatg gataccgggg aaattctcga gattgccgcg gaagtgctca agatcgaggc
    47641 cgaggggatt cttcacctgg tggaccgcct cgaggaatcc ttcgcccagg cggtgcaatg
    47701 gatctacggg tgccgggggc gagtcatcgc cacggggatc ggcaaatccg gcattgtggg
    47761 ccgaaagatt gtggccaccc tcagcagcac ggggactccg gccctctttc tccatcccgt
    47821 cgaggccgtc cacggagacc tggggatgat ccggggagag gacattgtcc tggccctgtc
    47881 caacagcggc gagaccaacg agctcaacgt catcctgccc agcctgcgaa acatcggcac
    47941 gcggatcatc tccttcacgg gcaagaccga ttcgaccctg gcccgacaca gcgatctggc
    48001 catctatgcg ggggttcccc gggaagcctg ccccatgggg ttggccccca ccgccagcac
    48061 cacggccatg ctggccctgg gggatgcttt ggcggtggcc ctgatcaagc tgagaaattt
    48121 ccaggagcgg gattttcacc ggtaccatcc tgcagggcac ctgggcgaga ggctcgacgt
    48181 gcccgtctgc gaagtgatgc gccggggaga agccatcccg gtggtgcacg aagatacgcc
    48241 ggttctcgag gccctggagg aaatgagccg aaagggcatg ggagccaccc tggtcctggg
    48301 gaagagggag gaactgctgg gaatctacac cgacggggac gtgcggcgca ccttgaaccg
    48361 gtatcccgac gtgcgggagg tgcccatgcg ccgggtcatg tccgtcaagc cccgtaccct
    48421 ttcgatgaaa tgttcggtgg ccgaggccct ggagatgatg gaacggcatc tcatcaccgt
    48481 tcttcccgta gtggacgggg aagaccggct ccagggaatc ctgcacctcc acgatctgct
    48541 gggaaaaggt cgaatccggt ttacccggtg atcgttcgcc gggcggaagc cggtgccggg
    48601 ccgctccgcg ggcctgcgcg gggtcgaagc ggttcggttg cgccccgggt ccgaaagctc
    48661 ctcgggacgt ctccccctgt tctcggagaa acaatggcaa caagagatgc ggcctccgtc
    48721 ggtcgaaagc ctctgcagga cctcgacggc gatccctttg cgcggccttc ctcgctcggt
    48781 gaatttcccc ttgcagcttc cgcccagaaa taatagtaga tagcatcttc ggcaaatggg
    48841 ctggcgtagc tcaacggtag agcagctgat ttgtaatcag caggttgcgg gttcaaatcc
    48901 catcgccagc tctcagttta cagactttcg gattttcgcc tgtgcacctc ctgtgcacgg
    48961 attccggagc ctcacgcatt ccatcaggcg caagccgccg ccatagcagg atgttgaatt
    49021 gacgggatga gctcaacccc tctttcccca agagaagccc aggcttcacg aagagtcccg
    49081 tccaccgaga agtgctccct tggcaggggc ccctgttctt cagcttttgc gagaacggat
    49141 ttcagaaaag agacggcaat atccgattcg atcaacctgt cgcgattctt ggagaaagtg
    49201 gaatggttcc aaacgggttc atccagatga agcccgataa accagcggaa aggccttggc
    49261 cgccgcgcct gataccgggt tgcgcccaag gtcgatctga catgctgagt gaagagtttg
    49321 agcaaatggc tgaatcctgc gggatggcag tttcggggtg aatgcaactt cgtatgagcc
    49381 ctcaagtccg gccggcattg atgaaggaga tctacgcatg gacatgtgga agttctttga
    49441 catcacgcat cgggatcaca ttgtctgcaa tccgatgagt cttgagaaac ttgaacagct
    49501 catcacactt ctgtctctga aacccggggc acgcgtgctt gatatcgcca ccggaaaggg
    49561 cgaatttctc atcaggcttg cagagcgcta cccacaaatg acggggacgg gagttgattt
    49621 ttctccctac tgcattgcgg atgtccgcaa gaagcatcaa gaacgtgttc cggatgccca
    49681 actccgtttc ttggagatgg atggggccaa gtatgtcccg gagaccttgg agagctttga
    49741 tcttgtagcc tgcatcggtg cgagttggat ttatggcggg catcggggga cgttgaacgc
    49801 gctgcaaaag atggcagccc cggaaagttg gatcgtggtg ggagagccgt attggcggca
    49861 tgaaccggaa agagagtact tggaagcgat cggagtgaca cggaacgact tcgggaccca
    49921 ttatcagaat gtagagatcg gacgagagca cggattggag gtggtctaca cactcgtaag
    49981 cagccgggat gactgggata gatatgaagg attgcagtgg tacgctgcgg aaacatgggc
    50041 gagcaatcat cgggatgatc ctgatgtgga caccgtgttg aagcgagtgc gtgcgagcaa
    50101 gaccgcgtat ctgatgtggg gacgagacac gttggggtgg gcgatttatg tgttcaagaa
    50161 gggggggtgt ggcccagttg cggttggctg acgcgcctga ttgggcatgc ccgggatggg
    50221 caggatgagg aaagaggcat caccggctcc gaacgagccg ttccacccgg ttcgggaagg
    50281 ggctgttaca ctttttgctg gggtgtcctt tctttcgatg ataggatcgt ccctcggctc
    50341 gttcctcttg cgcctcatcc cgggaatcaa gcaggcaacg ggttgggccc ggttgcatga
    50401 aagtcccgct ttcgtggaaa gcaagcaagt cctgcagcgg cgaaaagagg agttgcaggc
    50461 gctgccttcg cgaatagagg tggaaaatcg taatgtactt cgatcccgac ggcgatgaga
    50521 aacaatacga actgtatgat ctgaaaaccg atcccctcga gcggcacaac cgggccgacc
    50581 ccgaaaacct caagtactac gacccggtag agccggcgga gatgcaagac aagctccggc
    50641 gcaagatgga ggaaacccgc acgacgcccg cgcagcctta gccgggagag gccccttgcc
    50701 tcaacgggat gcgctgcccg gcgcggcctg ggccgccggg aggggtcttg ctcccctggg
    50761 gaggaatgcc gcgaggtgca tggaacaaga cgcccaaaac ccgccgtttg gctgcttccc
    50821 gaacaacctg ttcgacgccg tccgccttcc aggtgattgc gaaaccgtgc ggggcggcct
    50881 gcaacctttc ctgcgactat tgcttttatc tatcccggga aaggctctat cccggcgccg
    50941 gcctgcgaat gtcccaggac accctcgaga attacgtccg ccagcacatc gaggcaaatt
    51001 gcgccccgga ggtcaccttc cattggcagg gcggtgagcc gaccctcatg ggcctcgact
    51061 tctaccggag ggccgtcgag cttcagcgaa aatacgcaag gcccggcacg cgcatcctga
    51121 acacgctcca gaccaacggc acgaccctca acggcgagtg gtgcgggttc ttccgggaaa
    51181 accggttcct ggtcggggtc agcctggacg gcccgcgggc ccggcacgat gcctaccgca
    51241 aggggaaagg gggcgggccg accttcgacg ccgtcatggc cgggattgcc ctcttgcggg
    51301 aacacggggt ggagtggaac gtcctcacct gcgttcacgc cgccaatgcc gattgcaggc
    51361 tcgaagtgta ccgattcctg cgggacgagg tccgtgcctt catggtccag tttatccccg
    51421 tggtcgagcc cggcgccggg ggggaaggcg cggcaagccg ctcggtcacg ggaccccggt
    51481 acggcagatt tctcatcacg gtcttcgacg aatgggtgcg ccgggacgtg ggccgcgtgt
    51541 tcgtccagat cttcgatgtg gccctggggg tctggttggg cctgccttcc accctatgcg
    51601 tcttctccga gacctgcggg gatgccccgg ccatggaaca caacggggac ctgttttcct
    51661 gcgaccattt cgtggatccc ggccacaagc tgggcaacat cggccaaagg cacctggggg
    51721 atcttgtcgc ctcccctcga cagcgagcct tcggggagga caaatcccgc accctgccgc
    51781 gcttttgccg ccaatgctcc gtgcgctttg tctgcaacgg cggctgcccc aaggaccgtt
    51841 cgggcaccag ccccgacgga gagccgggcc tcaatgtcct gtgccaaggt ttccgggctt
    51901 tcttcacgca catcgaccgt cccatgagga tcatggccga gctccttcgc aagcgccgtt
    51961 cccccgcgga aatcatggga atcccccatg cagggaacct gcaacccgtt ccatgaaagt
    52021 cctgcattcc cgggaatctg cagcaaccgg tgaaggcgga atcttccagt tttctcgttt
    52081 caagcgctct tcgtgttccc ggagggaaaa ctcaaactct gggggcgccc cgggaatatt
    52141 tcccggatgc ctctttgagg gcgggcagag ggggcggagt cccgccccga aggaggttcg
    52201 ggtcccggga atccatcggt tgcaggaact gcggaatcca ttataggtta aggccatgca
    52261 aggggagacg gcattcgatc ggaacctttg ggaacgggga ccttgatgaa accggttttg
    52321 ttctgggcgg cattctggct cttttttcct gcagggatcc atgctgccgg gtcgctgaac
    52381 gggatcatct tcatcggcca gtccaatgcc gagggccagg gcgaactctc cgaccttccg
    52441 gaggacctcc tgcaggttcc cgacaacgtc gagtactggg acaaagagga acgcatttac
    52501 cgctacgacc agcaccgacc gcggcacggc ctggaggtga ccctccttcg aaagctctcc
    52561 aaggcaccca tgggggactg gttgttggtc aagtacgccg tggggtccac ttccagttac
    52621 tcctggaggc cggattggac tccggaaatt gcggaaatca cggataacgt ggaccagggc
    52681 ccctgctatc gcatgaccat ggatgccgtt cgtcgagccg tcatggagag gggaatcgcg
    52741 gtaaactggc gctatcttct ggtgttcaac ggggaaaggg atgcccggta tctcccggcg
    52801 gccaaaaact acgcctccaa cgtgaggcgg gtcctgggtg ccctgagaag ggatctgcat
    52861 tcccccggca tggaggtgct tatttgtcgg ttggatcttc cgatgaccaa ggggagaccg
    52921 tatgcggaaa aggtgagggc ggcgcaggaa accctgggtt gggtcaatct tgacgggtgc
    52981 agcaggatgc cgacggggac tcactatgac tcggggggcc ttcggctgat cggcctgcgg
    53041 gtctaccgga agatcgagca ggggctgggt ttcggttgcc tgccggggtc gtccgccgac
    53101 gggagccggc acgatggctc gattcgcccg cccatagtcc caaccctgcc gacaccagag
    53161 ttcccgggag ggtgcccggt aaatggacac gatcccgggg cattcccggc ggatgagtca
    53221 cgatcccaat tccttctcgg gctggaaata cttccgtagg ctcgatgcca ggttctcggg
    53281 cgtggtcaaa atgagctcgt ttctcaacag cttctggaga aaatagtcgt cggtgcgata
    53341 gttttcgtag aagttctggc gggagatgac gcgaaggaaa catcggaacg agtcgtcccc
    53401 ggcgtccggg ggacccgagt acagggtgaa attgaacgtc gagattccca gggacgcgta
    53461 cccccgcaga actgccgaaa gtccccgggc caggtcgcac aaatcctgcc tttccatctc
    53521 cgagaaatgt ttcttgccgg gaaagatccc caggacctcg ttggttccct ggggagaaaa
    53581 agcagcgatc cagtcaacag cccccgtgca ggcgatgaaa cgaaggccca gctcccgctc
    53641 cttttccgcc aaatcgaccc agtagctggt gccgtgtttt tccctgtatg cggcacccag
    53701 ggcgagcagc tcttccaggt gggtgaaggg aacgtcgctt cccagcacct ggaagtgggg
    53761 gtgaacgatg ctggcgcccg ccggtcccaa gtggttccca ttgagggtga gaaaggggac
    53821 ggcctcttcc gagcggcaaa gatgctccac gaattctcgg gttgccagga acgcttcccg
    53881 gacaagggct ggcgaaaagt cctcgagggg aagatggtgc ccggctccaa cccggatgac
    53941 cgcatggatc cgggagaccg ggaacaggtt gggaaacagg acggactcgc ccaccaccac
    54001 ccgccccccc ggtacgatct tctcgggata ggtcggggtc gtccgtttcc atcggtcctc
    54061 acagagaaag cattggggct cgctctcccg ggcgagtcgt tccatgagag cacggtccga
    54121 agcgccgaag aagaaggctg ccttgtcttc gagaccgggg ttgaagacgc tctgcctccc
    54181 cgtaagagga tcccttcgga tttcgagttc ctgggtgtcc agttggccgc ccatcatggg
    54241 gttgtggaac gaggaccgct gaaaatgcct gtgaaatcga attttctcca tggtttccgc
    54301 tcccgtcttc tcttcgaatt ctcgccggag ttctgccgtc gagcacacga cttccggggg
    54361 aaggcctcat tcgttcatcc attccagggc tcgttcgagg attcggttgt agatctcctc
    54421 gggactcatt cccatggctt tgggtacctt gaaggagtga tccccgccct cgatcacctc
    54481 gatccgatgg ggggccgaaa ggcgtgcaag cacgccggag agcatcggga ggtcgcacag
    54541 ggggtctcga gtcccggcga aaaacaacat gggaatcccg atcctgtaaa gaggctcgtc
    54601 ccggagggca tcctttctgc ccggcggatg gaggggatat cccaggaaga tcagccgccg
    54661 ggcggcaagc tgtccccggg ccgccatctg ggcggcaatc ctcccgccca tggactttcc
    54721 cgctgcgaag atccgtttcg ggcggggatg gacgtgagag ccaaggaatt ggaaggcggc
    54781 gtcccatgcg tcgtagagca aatcctgccg gtccggagta cgttttccct gctccatgta
    54841 gtggaagttg aatcgaaggg tgagataccc ggctcgggcc atcccctccg ccagaaagac
    54901 gatcatcggc tggttcatgt cgtttccggc accatgggca aaaatgattc ccgtctccgc
    54961 cccgtttccc ggaggggcgg caacggtccc tgaaacggtc ttcccatccc tgagggaaat
    55021 ggaaaccttt tcaactttca tcccgatcct cttctcccgg agttcagact cccgcttttc
    55081 ctgccggccc gtgatttccc gattccgggg ggatcgtgct cacaagcctg caatgtccta
    55141 tataatggaa gggttcgcgt ggcaagggca gggatcggtg tgaccgggga gggcatgaac
    55201 cctccccgca aagctgtgtg cggttgcgga cgcgtgtccg gcgatcaggg gccgacgggc
    55261 ttcgcctccc gggatgaccg cggttttgcc catgggctcg ccggggtatg gtccatgttt
    55321 ctcattcccg ggggtcctac tgcaccccgg cgggtttgca tcctcccgag ctttcctcgg
    55381 ctcctgccgg cggtccgcag ggagtgatcg ttttgaccgg ggctccctcc aggatggtca
    55441 ggctcacgtg gtagcccaca tacggataga tcaatccgta cccgttctgg ctgcgactgg
    55501 atgcggaaag aatgaagcta taggcgcagg tttccgacgc ccgccacttg gatccgacgg
    55561 gaacgatcaa gttgctcgcc ccgttccact tacccggacc gctttctccc acatgaccgg
    55621 aatagttgtc ctggtagatg ctctcgctga gattgttgcc gtggatggcg gtcagggcga
    55681 aggcgttgag ggccccccgg gcatccgtca ccgaaacgtc gaagttgaac ccggccggat
    55741 cggccccctg ggtgatgatg gcgcaggcgg gcaccgtttc gccctcgtag gtcacttcgt
    55801 tgattgtggg gataggggcg gtattgacca cattgagttt cagtaagttg ccgtcgggag
    55861 ccggcagacg acggccggcc gcgtcaaaca gttccagcct cagacggtaa aacccgtcgg
    55921 gcacctgccc gctctgccag ctgatcagga ggttgggcag gtaccacagg gtcgagggtg
    55981 acgggatctg gtacttcccg tcggcattgg gaccgatgga ggtgggaacc cacttgccgc
    56041 tgcccgagtc gaagtagaaa ttggtccacg tctgcaacaa catctgccag acagccccgt
    56101 tgtggtacca aacccggtag ctgcgagcat gatcgacggt gcgcagccgc ttccagtttc
    56161 cgaagatgtt gagcttgccg ccgaagggag catccgtggc cgcatagtag tcggcgatgt
    56221 tgaggttggc gttgcccaaa gcattgatgt acgtagaagg gaccatcccg accccggcga
    56281 aggtgggccc ggtgcttccc gcgggatatc cgggataggt tgcaagatcg atcaggtaga
    56341 atgagttggc aagatttggg aatatcttgt tttgcggctg ccccgacaaa aggtgggggg
    56401 tttgggacac cagggatacg tgcatccgga ccttggccat ttctccggca gacgtggtcc
    56461 atgtggccgg gtcgatcccc agctccgtca ggtccaatcg aaattcatag aatcgatggg
    56521 gtgtgctcga tgccgctgtg gccccaaaac cgtacttgcc cgccgacagc gcgctggttt
    56581 cccgacaacc cgtgtaggag gaggccgtct tataatatgc cttgatgaat tcccgaccgt
    56641 cctggcaggc actgtaattg aaatcgacgt cggccgtgac gctcgagttc cgatcggtgt
    56701 ccacggccaa cacgaaatag tccccgccat aggggtccgg agtagtgggt accgggtcct
    56761 cggtggtgtc tgccgtgaaa tccagcagca tgaacaggta ctgcccgttg ttcttggtgc
    56821 gcatctgccc gccggccagc ggggtcacgg tggccccgtt ccattcaccg ggggtaaagg
    56881 tcccgtcaat ggagggcgga gtgccggccc aatgccgcgc aacatcgatg tccgcaaagg
    56941 cgttggcggc cagcaacagc aggcaagcgc ccgtaaacaa ggcccatgcg cggcgtttcc
    57001 gggcaacagg agaaacggtg gtctttaggg tggttttcat ggtctcctcc ccttcaagag
    57061 tgaaggttca acgatgccgt tcatcaaaca acgggccgaa ccgttaccca tcggcggagg
    57121 gagcctagtt gtccgagctt gaaccgcgcc tggtgcgaag aaggagctct gatccgccca
    57181 ttgtcactct ccggcccggc catcccaccc cctcacgggt ggatccgtcg agccaaactg
    57241 aaagacactt tgagacactt tcggtgcacg ttcagagcac tccggaccgc atgagccagg
    57301 attcgacaga gtagaaatac ttccgagaaa gcatttgaga aaataatggt atcactaata
    57361 gccgagccga agccctaagt caaccattgt gcgcaggagt catgctccag agtatggtac
    57421 catactgaaa catccgtatg ttcgcaggga aggcgggcac atgagaactc cgcctgagtt
    57481 gagcgattcc gaacgcatca gggcttcatg ccggcgacgg cgggcatccg gcggggatgc
    57541 acgctctttc cggattcccg catgcgcggg aatgatgaaa cgggatccct gccggcaaaa
    57601 tcgctcaatc tagggtacgg tctctccggc caccggggga tatcccggtt ttcgagattg
    57661 ttcaccaagg gagagaatgc acatggcaac tacgactctg accttcgatg tgggggatgg
    57721 gattggggtg attcgcctca accgccccga ggacggcaat gcgatcatcc tggaaatggc
    57781 ccgcgaactc ctggatgttg ccgatcaatg cgacaaagac ccggcggtcc gggccgtggt
    57841 gctgacgggc aacggcagga tcttctgcgc ggggggagac ctcaaggtct ttgcggctcg
    57901 gggggaaaac gcctcccgtt acatcgaaga ggcggcccgg acttttcatg aggctttatg
    57961 ccgattcaac cggatggacc cgccggtggt gggggccatc aacggggccg cggtgggggg
    58021 cggtttcagc ctcgccctga gtaccgacat cgccgttgct gcggaatcgg caagattcag
    58081 catggggtat acccggatcg ggttgtgccc cgacggcggt tgcagctact ttctggcaag
    58141 aatggtcggc ttgcgccggg ccaaagaaat cgcgctgcta aatccggtgt tctcggccag
    58201 gcgggcaatg gaatggggcc ttgtcaacct ggtggtcccc gacgatcaac tcatggccgc
    58261 cgccctccag atggcccgtg gatgggcgga aggacccacc cgctccttcg gggagaccaa
    58321 acgcctaatc ttgtccggtg cggccgatag cctggaatcc cagatggaaa gggaatcccg
    58381 cgccatagcg gccatggcgg gcactgccga cggacgggaa ggaatcgacg ccttcctcgc
    58441 caaacgttca cccaagttca cggggaaata gcggcttcca aaggcttggt gtcaattcag
    58501 ggatttttta gcaaacgcga agacttgcac gggtcccgtc gttctcggcg aattcggacg
    58561 ggagatccgc ccgaacggga ggaagacgaa acgccgaagg gtccccgggc acgcggaatc
    58621 cccgttccca tggggctgcc cccgtccgga atcccaaccc cgaggccaac ccccgctact
    58681 tgtagatggt gaggttccgg gtgctcgagc tgaagacctc ccggtccacc atcacgttga
    58741 tgcaggcagg ttttcccgat tctcgggccc tctcgagggc cgggcggatc ccggacgggt
    58801 cggtgacgtg ctccccgtgt ccgcccatgg cctcgacgat tcggtcgtag cgaagcggcg
    58861 aaagcaggtt tgccgtattg cccctttcgg gaccgtactt gttcagctgg gcaaagcgta
    58921 cctggttcca ggcggcgttg ttggccacaa tgccgatgac gggcaggttg aaacgcacga
    58981 gggtatcgta gtcgaatccg gtcagcccga aggcgccgtc gccgaacaga accaccactt
    59041 ccttgtccgg catggcggtt ttggccgcga cggcaaaggg agtgccgacc cccagggtgc
    59101 ccaggggacc ggggtccagc cagtgccccg gcttccgggc ccggatcacg ctcgccgaca
    59161 tggtgaccac gtcgcctccg tccacgatga ccacggattc gtcgcccaga aattcgtcga
    59221 tctcccgggc aagccgaagg gggtgaatgg gcacggcgtc cgaatgaaga gaaccccggt
    59281 cttgagcttc gaggtcggct tcccgccccc gtagttcggt cggccaatgt tcgctctcgg
    59341 ccgaagccga gaccctttgt gcgagctctt cgaggaccgc ggacgaatcg gcctggatgg
    59401 gaacctccac ggcgcggttg cggcacagtt cgctgtaatc caggtccacc tggatgatcc
    59461 gggcatccgg gccgatgctc ccgccgtagc ccagcctgaa atccaggggg gtgcccagga
    59521 tcagcacgac gtccgcccgg gagagagcgt accggcggga acggctgaag aggcgcggag
    59581 agtcgggggg caggcagcct cgggcggcac cgttcaggta ggtggggacc cttgcctttt
    59641 ccacgaaagc aagaagttga gcggccccgc ggcaggtcca aacctgggtc cctgcgagga
    59701 ccaccgggcg ttgggctcga gaaaggagtt ccaccgcacg ttccatgagc tttgggtctc
    59761 cggtcatccg gccttgggag cgggtgcgta cgggaatgtg gacctcccgc tcgtccacct
    59821 ccccatcgag gacgtcctgg ggaatctcga ggaaaacggg tccggggcgg ccgctgaagg
    59881 cctcgcgaaa agccgtgccg atgatctcgg gaatccgttc ggtgctcaag accgtgcagg
    59941 aaaacttggt gacgggcttt aggatgtcca cgtgattcat ctcttggagc ccgcctttga
    60001 gcagtttgcg gaccggggcc tggcctccga taagcaacat gggcacctgg gctcgaaagg
    60061 cattggccac tccggtgact gcgtccgtta cccccggccc cgcggtgacc accgccactc
    60121 ccggaacccc ggtcaaccgg gtccaggcgt ctgccgcatg agccgccgtc tgctcgtgtc
    60181 tcacgtcgat gatgcgaatg ttttcatcca gacagccgtt gtagatgtcc atgacatggc
    60241 cgccgcagag ggtaaatacg gcttcgaccc cttcctgctt gagggctttc gccaccagtt
    60301 gtcccccgga tacgttcccc atgggtgcct cctcctaaat gctctgtccg aatccagcga
    60361 ctagacggcg caaaaccttc ttaccacggc ttcagggtga acggtctgga agccttgatg
    60421 atctctcccg gtttcttgtg cgccacctgc accatctgtt cggcccactc gtcgaggatg
    60481 acatcggctt ctccatacca ggtgagtgcc ctcaggttga tgggcgcaaa tttcccctgc
    60541 tcccggtccg caaacattgc cagcaccgtg cccgtatccg tatccttgat tctggcctcg
    60601 aaagcgacgg tggccttggc ccccgatcct cttgccgctg cctgaatcgc cactccggac
    60661 ccgtaaggag cggcaatgga catggcttcc agaacggggt tgctgggaac cagttccgtg
    60721 agtgcgaatt ccatggtgag agaacccggt tcaggggtgt tcacaacctc gaaacgtttc
    60781 agcggatcct ctcgaaaggc cgcttggaac ctttgccgca tggatcgtgc gatgttcttc
    60841 acgtcatctt ccatttcctc gcggcgtaag ctctgctgcc accagtttgc cttcaggagg
    60901 tgttgaacgt tgacttcctc gatgtatagg gttttgtacg ccttccagtc cacgccttcc
    60961 ttggcccaga cccggttgaa cgggaggtct tcccggtggg acaattcatt catcgggaca
    61021 aagccggctc cttcggaagg cttcgccttc atggattcgc aagccgtgag gccgacactc
    61081 agcaggaccg cgaggaccaa taagctccgg aagcatttgc cgctcatgat ggattccttt
    61141 ccgggttcgc cgcgaggcaa tccgatgtac cctttgtcgt gtctcgatta tcccatggat
    61201 cccgttcgat tcatgattct taatccatga ttcgccggtt cacaacgata acttgatgct
    61261 aaagaggcaa agatcgggaa ttgaggccgg gtgtgagacc ctgacggcgc ctccaaggac
    61321 cgggctctcg cctccgtccc gtggcatggg tccaggaggg cgaatcccgt cgagggggga
    61381 cgaaccccct gtttccttgc cccgggatgg gaccccaacg aagcggcagc tgcgaggagg
    61441 cgggcagaga ggaaatgctg caaggatcga ggaggtgtgg taccatgcct tcccggattc
    61501 gatccaccgc agaccgcgcg gcatcttttt ccaaggagtc ggtcatgaaa atccttacgg
    61561 cacctcgaat ggagaggtgc atcgggtgtc actcctgttc gttggcctgt gcccggctgg
    61621 tccacggact tctctcgtgg gacgcggcag gcatccggat ctcctcttcc ggggggctgt
    61681 cgacggggtt tgaggccagg acgtgccttg cctgtcttcc cgccccctgc gttgcggtct
    61741 gccctaccgg ggcctactcg caacgaaagg gcgggggggt gatcgtgaag aaagccctgt
    61801 gtatccgttg cggcgagtgt gcccgagcct gcccggtgga tgcggtcttc ctggatcctt
    61861 cgggggggcc cttcgtgtgc attcattgcg ggcgctgtgc ggctttctgc ccccatgatt
    61921 gcctggaaca cggggaagtg gaaactcgag ggccgaggga gaacgatgtt ccggggaggg
    61981 cgtcatgatc cgggaggatt tcagggttct ggtggtggac cttgccgccg ggcggggcag
    62041 catcgtccgc atggagggcg tggacgccgt ggcgggcggg agcgggcttg cggccctgct
    62101 tttccacaag tacggcttgc ccgacaggcc gtggaacgac ccggaacagc cgctgatatt
    62161 cgccataggc ccccttaccg gctatttccc cctcatgagc aagaccgtct gcgccttcaa
    62221 atctccctac cacgatcaat acgccgagag ccatgccggg ggccgatcgg ccctctccct
    62281 tcggttcgcg gacctggacg ccctggtgat ccggggccgg gcgacggagc tctcgtgcct
    62341 gtcggtgggt tcccggcacc tggaggtcaa ggatgtccac ttcctccggg gcatggatct
    62401 tcttcgaacg gggaaaatgc ttcggcgcat gtacccggga gccgggcatc gcagtatttt
    62461 tcgcatcgga cccgcggggg agaatctgtc ggctatggcc tgcatcaacg tggataccta
    62521 ccgtcatttc gggagactgg gatgcggagc cgtcatgggc gcaaagaatc tcaaggggat
    62581 cgtgatcctg ggcgacgggt ccttccccct tcctccgggg aaagactacc cccggctctt
    62641 cgccgaggtg tatgggaaga tcacggacac cgatatgatg gaaaagtacc acaactacgg
    62701 cactccggtg aatatggcgg tcctgaacgg aatccgcgcg cttcccatcc ggaaccttca
    62761 gaaaacgagc gatcccgaca tggaagggat cagcggcgag actttcgccc gggacaccct
    62821 gctgcgcaac gccgcctgcg cggggtgtcc cgtgggttgt gttcacctgg ggtttgtgcg
    62881 ggaacggttc atgggagaaa accgctacct gtatcggcaa gtctcctacg accacgagcc
    62941 catttttgcc gtcggcgcca tgctgcagat caccaatgcc ttttcggtgc tttccctcct
    63001 tgaggcggtg gaacgggagg ggctcgacgt catgtctgcg ggagtcgccc tggcatgggc
    63061 caccgaagcc ctggaaaaag aaatcctctc ggttcgggaa accctggttc ccctgaaatt
    63121 cggcgacgaa gggagttacc ggcaagcatt tcaccacctg ggggccggat ccaacgattt
    63181 ctatcggctg ctggcccagg ggaccttaaa ggctgcccga gcctacggag gcgcggattt
    63241 tgcctgtgtg ctgggacaag agatggcggg ctatgccacg ggggaggtct tctttgtatc
    63301 ccaggccctg ggtcttcggc attcccacct ggatgcgggc gggtattcct acgatcagaa
    63361 aaaccaggca ccggatgtgg aaggagcggt ccgatttttg gttcgggacg aacaggcaag
    63421 ggttctcttg acctccatgg taggatgcct ctttgcgagg gggatctacg gagacgaggt
    63481 cctctccgac tgcctccgtt ccctcggccg taccctcctt gcggacaacc tgggcagggt
    63541 ttcccggcgg gtgcagcaac tgcggtggaa aatccgcctg ggtacgggct tcgacccccg
    63601 aacggtgacg atccccaggc gtttctacga agtcgaaacg tggaggggca ggatcgatgg
    63661 gacctacctg gacgccctca aggacgccta tgcccgggcc gtcctggagt tggctcacgg
    63721 gggagccgac taacgggctg cccggggaaa aggcgattcc tgccgcggcg gggtggaaat
    63781 tggaggtccc ggacggatcc acaaaatttt aagttgcctt ttgagtttcg ttctgctaca
    63841 tggaaaagct tacttgccca tgggttgcat tgcctcgtgg tcaagcgact gtggagaggt
    63901 acccgagcgg ccaaagggag cagactgtaa atctgccggc gaagcctacg gaggttcgaa
    63961 tcctcccctc tccaccaccc atggaacaat ccatgtagga gatggtgccg gcttccagcg
    64021 ggtcattgtc gatggcacta catgtggtga aacgggaacg ttggatgctg gttttcatgg
    64081 atccgtcgcc ccccatccag tcagggttcc cttgcgtgat cgacgctctc cagtgtaatg
    64141 gctgctttct ctcgagccca cgtagctcag taggtagagc acttccttgg taaggaagag
    64201 gttcaccggt tcgatcccgg tcgtgggctc cattcaccgc tggaaaacga gaaggcaggc
    64261 atcgccgtca tgctcccttc ggaccggccg ctacgcattg tccaagctcc acatataggg
    64321 tgagagacat ccgtgcacgg aatgcaatga aacaacgttt ggaggaggaa ctaccatggg
    64381 gaagaagaaa tttgagcgga cgaagccgca cgtgaat
//
LOCUS       DAOOVC010000006        63635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_252_length_63635_cov_6.884712, whole genome shotgun sequence.
ACCESSION   DAOOVC010000006 DAOOVC010000000
VERSION     DAOOVC010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 63635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63635
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_63635_cov_6.884712"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1405)
                     /locus_tag="PLM79_01480"
     CDS             complement(83..1405)
                     /locus_tag="PLM79_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85002.1"
                     /translation="MEQAVLNPMLKNDVKDYLSKFNLSLCLTCGTCINGCPVTGGEGA
                     EGLDARKVLRMLAYGMVDEVVQSKFPWLCTGCGRCVHACPMGIDIVGIMGHMKHLRPR
                     DQVPGILHKGVENVLNTGNNMAIPKEDYFFLMSDMGRELAGELPGFYVPIDKDEAKIL
                     FFPNSKEVFSDNDDMIWWWKIFYAAKEDWTIPSENWEAVDWGLFTGNYEATKILAQRK
                     IQFMKQHRIDRMIMPDCGGGSYGCRKGMKECVLEDPNNQINHIYLYDYLKEIIETGRI
                     KLDKSVHAGKVFTWHDSCKHGRELERNFGKGYFDEPRWIIEQCVDHFVDMQPTRMNNY
                     CCGAGGGNWPMPYEKESAYYGRHKVRQIKDAGANVVVVGCSNCHDQLMKRLPKYHPED
                     CKYEVKYLWELVADSLVIEPWSDEEVARAEEEAKAQWERLGVEMEDEE"
     gene            complement(1436..1591)
                     /locus_tag="PLM79_01485"
     CDS             complement(1436..1591)
                     /locus_tag="PLM79_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85003.1"
                     /translation="MDRLRLLSSLSCDWWVLDLREEAALLSTPRLVRELMNTVPGDEP
                     AEYFGMI"
     gene            complement(1725..2222)
                     /locus_tag="PLM79_01490"
     CDS             complement(1725..2222)
                     /locus_tag="PLM79_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HOV85004.1"
                     /translation="MCNFIPDVDVLKKAAMAHGFSGVDWTFKPEDLPTNGLDESRLLG
                     KISRLHPLEVHRAFKGIDFGDADEAGASAAMETHRKVCRMVSRLEDRFMTVHLGLGSR
                     STGELSWERGIEAMTELVEYADDFGICLCLENPASGWSSPPELFEKLIRKSRAGVTLD
                     ITMPA"
     gene            complement(2449..3240)
                     /locus_tag="PLM79_01495"
     CDS             complement(2449..3240)
                     /locus_tag="PLM79_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV85005.1"
                     /translation="METKIKNLLEEGRVDGFWAYKTAHGFPVPWLFTNENAHELEPWQ
                     PTSARYPLLKLLLALSRQNPEKIYGILVRGCEERGLQELFKWSQLDRNKVIALGQACS
                     EEMALYCECRQPYPDRLDYGRAVAPVSKNDRIEGLHAMEMDERLDWWRTHMNRCIRCH
                     GCRDVCPVCFCTECSLQHPDLIPGTELPPDTSFHLVRAVHMGGRCIDCGLCEEVCPAR
                     IPLRSLYKEVNRLVEEIYGYTPGTEGAMSPFTFLGEELLLPQGPQ"
     gene            complement(3296..3763)
                     /locus_tag="PLM79_01500"
     CDS             complement(3296..3763)
                     /locus_tag="PLM79_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85006.1"
                     /translation="MREREFEPKILAFLCNWCSYAGADLAGVSRLQYPPNIRVIRVMC
                     SGSVSLHHLLHAFQKGADGVLVAGCHIGDCHYLKGNYMTLKRVKFIEGLLRFAGYDPA
                     RLRLEWISAAEGIKFAEVVRDFTSRIQTLGPTPSFEAIPQYGRQETEVGSRTA"
     gene            complement(3787..6828)
                     /locus_tag="PLM79_01505"
     CDS             complement(3787..6828)
                     /locus_tag="PLM79_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85007.1"
                     /translation="MSKSSENMIGSAMVIGSGIAGVQAALDLADTGYFVTMVEKSEAI
                     GGVMSGLDKTFPTNDCSMCILSPKLVECGRHPNIEILTLTDVSEVLGEAGNFTVSLHQ
                     RARYIDMGKCIACGLCAEKCPRKVADSYNLGLSRRKAAYVKYPQAVPLKYAIDKDNCI
                     YFEKGKCRACEKFCPAGAVKFDDKDRDFTRKVGSIILAPGFQAFDPKIFDTYNYAHFP
                     NVITSMEFERILSPSGPYQGQLVRPSDRKEPHRIAWLQCVGSREMNRCDHGHCSAVCC
                     MVAVKEAVIAKEHSREPLDTAIFFMDMRTSGKDFEKYYDRAREEHGVRFVRSRIHSVE
                     RVTGSENLNLRYTLDDGVVRQEEFDLVVLSVGLETSPEVMDLARRLGIEVNEGRFAST
                     SPFAPVVATRPGIFVCGAFQGPKDIPQSVMEASAAAAETAALLAPVRGTVARKPEEPK
                     EMDGANLYPPRIGVFVCHCGINIGSVVNVPDVKEYAKSLSGVAYVEESLFACSQDTQE
                     RMKEAIREHKLNRIVVASCSPRTHEPLFQQTIREAGLNKYLFELANIRDQNSWVHQAF
                     PEAATAKAKDLVRMAVARVSLQQPLEELRLNITKSGLVLGGGVAGMEAAAALAGLGYP
                     VTLVEKKKELGGHALKLLTVWNGEPVRPYVDALIEKVETHPLITVRKGAVLKEVKGFV
                     GNFVSVLDVGGKEEAVHHGVAVIAVGAHSYKPSEYAYGKSPKVFLNLELDEAIRDRDQ
                     AVVSARSAVFIQCVGSREPDRPYCSRVCCSHSIGNAVRLKAINPDMDVYVLYRDIRTY
                     GLRENTYKEARQKGVIFVRFDPDRKPRVEIEGYTPRVSVFDPILRRSVVIKPDILCLA
                     SAIETREVDAPAKMFKVPLNPEGFFLEAHMKLRPVDFATEGVFVAGLAHWPKPTEECI
                     AQAKAAASRASTVLARDFIAAGGVAAVVQKELCSGCRACVECCPFGAIGTIEGEAKCE
                     VNQALCKGCGTCAATCPSEAVSLMGFSHLQLYRQIDEALSA"
     gene            complement(6843..8282)
                     /gene="gltA"
                     /locus_tag="PLM79_01510"
     CDS             complement(6843..8282)
                     /gene="gltA"
                     /locus_tag="PLM79_01510"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOV85008.1"
                     /translation="MSEAKETGLPPKEAESMKSKMNLNRVGMPKQEPEVRRRNFDEVA
                     TGYTYEMAMEEAGRCIQCKKRNCRQGCPVNIDIPDFIRLLQEGNIEGASKVLRTKTSL
                     PGICGRVCPQELQCESSCTLNKKGAPVAIGRLERFIADWDRVQGAVTTPEIAPSTGKS
                     VAVVGTGPSGLTVASDLAVRGHEVTMFEALHVAGGVLMYGIPEFRLPKEIVQREVQYV
                     ASLGVDIQLDTVIGINYSLDELLNSRYQAVYLATGAGLPQFMRIPGENLNMVYSANEF
                     LTRTNLMKAYLYPDYATPVKMGKRVAVIGGGNVAMDSARCAVRLGASEVYVIYRRTRQ
                     EMPARLEEVENAEEEGIEFHFLCNPTAFISDGRNNVSAMELIRMKLGEPDASGRGRPV
                     PIPGSEYIMDVDTVVVSLGTSPNPLIASTTPDLETSRRGTVVTDKSGKTSKARVWAGG
                     DIVSGGATVISAMGEGRRAASAMHEYLNR"
     gene            complement(8318..9163)
                     /locus_tag="PLM79_01515"
     CDS             complement(8318..9163)
                     /locus_tag="PLM79_01515"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOV85009.1"
                     /translation="MFSIVAHEELAPMVHRMTVQAPVVAEKARPGQFVIILVDEKGER
                     VPFTISGWSREAGTVDFVYIEVGKTTRALAALKAGDHLAHFVGPLGKPAHVDHFGHVV
                     AVVSGYGIAATAPILDAYKEKGNRITTILQAPDRERLFGKAELEAVSDRVILAVGTNG
                     EDMATSTTRPLRRLLADHPQTPTDRVIVMGSICLMRVISELTRPYAIKTIVHLTPLMV
                     DGTGMCGACRLSVKGSNRFACVHGPEFDGHEVDGWDVLMARRCTYAHESILQQSFQCR
                     SCSQW"
     gene            complement(9174..11858)
                     /locus_tag="PLM79_01520"
     CDS             complement(9174..11858)
                     /locus_tag="PLM79_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV85010.1"
                     /translation="MNSIKMDSIKLWINNQEVEMEPGRSIMEAADQAGVHIPRLCYHP
                     SLKPSGACRLCVVEIDGCRGLPAACSTPVEQGMKIRTQTPKVTEFRREVLRLVLQEHP
                     RECLGCPRNGTCELQRLVEAVGIDFPYPPPNGERPPVQAAGSYFERDMSLCVRCGRCV
                     RICHEVRGSKAMVFLEIEGRQEVGTALGRSLEESGCQFCGACVDVCPVGALREKLEIT
                     HGEARGQMLDACEALTSIVMNLYRKEMPTQWKTSICPMCSANCRMAFELSESGEIIRV
                     RPDRNRKGSHGQACVQGRFLLKSYLQRPDRLNKPLVLENGRYREAEWESVLDELTERF
                     RSYGPWETAVLTDAGLATEELFLLQKFARTALRTNLVGCITPSGQATAEEALSRHSNP
                     SALKGNIRELSKAGTVLAIGVNPPASHPIAGTALREATLNGTKLIVANPLSIGLSRYA
                     DVNLHYLPGTEAVLIGGLIRFLLDENHEDPAVVARNPQAVSTLQKSLASYEPEAVAGI
                     TGVSLETLTDAASLLGKRKPLTILLGLGLVQSPSVRESIDAMVTLLHLTGSVSKAGGG
                     MIRPYGNANLQGASDIGLVSQLFEARGQGSGCGVPPSGDVFEMLASSQIKAVYLAFET
                     YENSLFDSLRPFLDSLELVVLQDTVLPPSQSREQGRWPHVVLPMASVMEKGGTFTTSE
                     RKQIDIGPVVSPQGEARSVLWLLRELAQRMKVPGFGSQDEEAARDEIWKEIAASTTLV
                     RRSQKVSSGCSCCGTPVKLKLPSDCQNDMPEWKPVPLWTPEGFRSNGVPFAAVAKEDL
                     EPYFLGPLLAPEAKTVFYPDCEVEMNPSDLFGMGLMPGDTVRVVTHEGLEQAGRLGVN
                     PLLPPTVVAAPMALLASLKKELDSPVRIVSVRVEKTGE"
     gene            complement(11815..12756)
                     /locus_tag="PLM79_01525"
     CDS             complement(11815..12756)
                     /locus_tag="PLM79_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOV85011.1"
                     /translation="MLALVNVPGYSDSLKNTIRGLSVDLARLIVAADDGVMPQTLDHL
                     EVLNFLKAGSGMVVLSKADLVDEETLELAELEIREVLAGTFPEDRPVIPFSAVDRRGA
                     EEVRAAIEAVAPDVSGKTDDTPFRLRIDKVRSFPRFDTVASGTILAGSTSEDGPVQLL
                     PSGKETKARFIQVHYERVDRAASGQRVGINLHKISIDDVSVGVALAAPGSLRSGYIPN
                     VGISVLPKARKAVVNRQRVRLYAGTCGMNALVVLMERQRLNPGESGLVRFRLHEPLIV
                     LPKDPFVICPIYAAHSVIAVRGEDEFDQDGFHQAVDQ"
     gene            complement(12936..13496)
                     /locus_tag="PLM79_01530"
     CDS             complement(12936..13496)
                     /locus_tag="PLM79_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85012.1"
                     /translation="MADIISLQCHKSRKAADRGFREWRRLFRSVTDIDEHTKWADLPD
                     EIVLFFCEEDPESKHALYDLLMGAQRLGTGHDLEAQAFDTFSTLMNAYFFITDQARFE
                     CMRRLGWIEAIPRGDRSIIDLVMDSATYEYAAQLEAPVPASTHPAYEEDRQSRGIDRP
                     ALVRKYIAQAIRLFKERVPLESSATQ"
     gene            complement(13520..13981)
                     /locus_tag="PLM79_01535"
     CDS             complement(13520..13981)
                     /locus_tag="PLM79_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85013.1"
                     /translation="MEDAFEPVIIGFCCQWCSYSAADLAGSMRLQYPTNVKIIKVPCT
                     GRLDVIFFLKALEEGADGVFASGCLLGDCHYISGNYRAKKRVAYVKELLAQIGMDPER
                     VEMYHNSAGMGPQFAQCCRDFTDRIRKLGPGLRRKKQETGSIQAASGSVAG"
     gene            14793..16691
                     /gene="dnaK"
                     /locus_tag="PLM79_01540"
     CDS             14793..16691
                     /gene="dnaK"
                     /locus_tag="PLM79_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOV85014.1"
                     /translation="MAKAVGIDLGTTNSVVAIWEDGKATVIPNSEGSRTTPSVVAYTE
                     DGQRLVGQVARRQAILNPSGTLYSVKRFMGRKWGEVDEESKIVSYKLVPGPADAVRFE
                     VRGRKISPEEVSAQILRKLVDDASRHLGERVTEAVITVPAYFNDAQRQATKDAGEIAG
                     LKVLRIINEPTSAALAYGLEKRKNETILVFDLGGGTFDVSILDVGDGVCEVRSTSGDT
                     HLGGDDFDKRVVDWLAEEFKRETGLDVRGDRQALQRLYEAAEKAKIELSSTTETHVNL
                     PFITADASGPKHLVMKLTRAKFEDLVHDLVQRCMKPVRQALADARLTEKDLDEVILVG
                     GSSRVPAVQELVRQLTGGKQPNMSVNPDEVVAVGAAVQAAILKGEVEDVVLLDVTPLS
                     LGVETLGGVMTKLIERNTTIPARREEIFTTAEDNQTGVEIVVLQGEREMARDNRVLGR
                     FRLEGIRPAPRGVPQIRVVLDIDANGILHVTARDQETGLEQKVTISESTNLAKNEVER
                     MVREARQYAAEDKERRLTVEAKNQADSMAYQLDRMLSELGDRVPVHERARGEQLIREV
                     RRAVREEGTDRGQYQQLAGDLQQALQMLTAAAHQEASSASAGGGQARYGSRGRGGDDV
                     IDAEFSER"
     gene            16763..17752
                     /locus_tag="PLM79_01545"
     CDS             16763..17752
                     /locus_tag="PLM79_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOV85015.1"
                     /translation="MAIKFKDYYKTLGVSRTASQEEIQRAYRKLARKYHPDVNKASNA
                     EEKFKEINEAYEVLKDPDKRSKYDQLGPDWQGGQDFRPPPGWDFQYDFGHGRGPEQGG
                     FQWSGGTGFSDFFEALFGGQGFRGAGGRGARKGPVWSQAGSDQEATIRISLEDAFHGA
                     TKSIALQVQTVTPEGQLSIQSKNYDVKIPPGILPGQKIRLGGQGGEGIGGGPRGDLYL
                     KVEIEPHPVYRLEGRNLFMDLPVAPWEAVLGVEARLPTLSGAVTVKVPPGTQNGRKLR
                     LRARGMPNSRGAPGDLYAVIHIMVPTKPSARERELFEELRKTSRFNPRSGEEG"
     gene            17759..18097
                     /locus_tag="PLM79_01550"
     CDS             17759..18097
                     /locus_tag="PLM79_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HOV85016.1"
                     /translation="MADRKYFMVQVGRYSRSFPFLTADEVAFRCGVHPELVERFLQLG
                     LIEIAEKDWNGEPLFPMDVVPMVRKILRLRNELGVNYAGIGVILELMTRIESLEARIR
                     ELEDELFGGV"
     gene            18189..20804
                     /gene="clpB"
                     /locus_tag="PLM79_01555"
     CDS             18189..20804
                     /gene="clpB"
                     /locus_tag="PLM79_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOV85017.1"
                     /translation="MDLSKFTIKSQEAVQAAQAKAIQYGHMEVDGEHLLLVLLDQPGG
                     LASRLLQRMEIPAERVRERLEEELERKPRVSGPGIEPGKVYISQRVSKLLVQAQNEAK
                     RLKDEYVSVEHLLLAFVDEGAATPAGRVLKEFQLTKDLLLKTLHEVRGRQRVTSADPE
                     TTYEALQKYGRDLVQEARSNKLDPVIGRDGEIRRIVRILSRKTKNNPVLIGDPGVGKT
                     AIVEGLAHRIVRGDVPEGLKDKTIYALDMGALVAGAKYRGEFEERLKAVLQEIKESEG
                     RIILFIDELHTIVGAGKAEGSMDAGNMLKPLLARGDLHCIGATTLDEYRKYIEKDAAL
                     ERRFQPILVDQPTVEDTISILRGLKERFEVHHGVKIQDSALVASAVLSNRYISDRFLP
                     DKAIDLVDEACAMIRTEIDSRPEDLDEVSRRVMQLEIEEVALKKERDRASHERLETLR
                     KELAELRSRADAMNAQWESEKQAIKKVQALREEIEKVRREIEVAQRNFDLHRAAELTH
                     GRLPDLERRLTAEEDRLSSEHGGNRLLREEVTEEEIAEIVSRWTGIPVAKLVEGEREK
                     LLRLDEVLHRRVVGQDEAVRLVADAVIRARSGIKDPRRPIGSFIFLGPTGVGKTELAK
                     TLAEALFDSEDNIVRIDMSEYMEKHTVSRLIGAPPGYVGYEEGGQLTEAVRRKPFSVI
                     LFDEIEKAHPDVFNVLLQILDDGRLTDAQGRTVDFKNTVIIMTSNIGSTHLIEGVTEG
                     GEIRESARKQVMAELRRAFRPEFLNRVDDIVLFKALTLQEIEKIIDLLTQDLVKRLKD
                     RRIALDLSDEARQFIARAGYDPVYGARPLKRYLQHRLETTIGRALLSGDIPDGSTIQV
                     GVRDGELHVTHTSAQ"
     gene            21466..22815
                     /gene="dnaA"
                     /locus_tag="PLM79_01560"
     CDS             21466..22815
                     /gene="dnaA"
                     /locus_tag="PLM79_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOV85018.1"
                     /translation="MDQQWRQIKGELEKVLSRGQYDLWVTTIEFLGVQDGRLCLGCRS
                     RFHIEWLRGKLENKLLDTARAFFPQVRRVEYEVVKDVEDPPAEAREDWPRQLSFHEVV
                     RRPEPVYNRRFTFDQFVVGGCNQFAYAACLGMASGQCLYNQSVYLLSETGLGKSHLSH
                     AVGNYLLSSKPETRVHYVTAEQFANEMISALRNGSMEVFKSKFRADCDVFLLEKVEFL
                     SGKEKVQAELVYTLDELMNRGKRILCTGSSHPKQIPKLNSDLQSRLSGILVAPIEPPD
                     FETRVEIIRSKAKGENLVLPRGVAEYLAERVSGDVRRIESCLVGLIAKTNILGVPLTL
                     QLAEEVTQTMLENLPKIDMQRIQEVVCSVFQITLEDLKSPSRRKEVAMARKMGMYLAR
                     MYTSESLVSIARSFQRSHSAVVHAVKDLTREMEDSGSKVRRQVEYVSRRLETTCLTS"
     gene            complement(22882..23169)
                     /locus_tag="PLM79_01565"
     CDS             complement(22882..23169)
                     /locus_tag="PLM79_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOV85019.1"
                     /translation="MTLTKAHIVENLFAKNIFTKTESAHIIETLFEIVKQSLEDGDDV
                     LISGFGKFSIKEKNQRRGRNPQTGEPIMLSPRKVVTFKCSGVLREKINRAK"
     gene            complement(23330..23406)
                     /locus_tag="PLM79_01570"
     tRNA            complement(23330..23406)
                     /locus_tag="PLM79_01570"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23370..23372),aa:Met,seq:cat)
     gene            23682..24065
                     /gene="queD"
                     /locus_tag="PLM79_01575"
     CDS             23682..24065
                     /gene="queD"
                     /locus_tag="PLM79_01575"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOV85020.1"
                     /translation="MNPGVYEVKVISDFSAAHLLRNFRGKCENLHGHNWKIEVVLRGS
                     KLNEAGLLMDFGEIKHATRDLLGEIDHKYLNDLPSFKDQNPSSENIARYLFEQLSVRL
                     NTESRYVFSVSAWESADACATFVRE"
     gene            24103..25257
                     /locus_tag="PLM79_01580"
     CDS             24103..25257
                     /locus_tag="PLM79_01580"
                     /inference="COORDINATES: protein motif:HMM:NF021600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HOV85021.1"
                     /translation="MALRKRIHPVVKQRDLHRRPMVFVLGALLTCLVVMGVSQHVWAL
                     QRPGGAAESPGGGAGTAAAPVATLPAPETPDAHASAAVQKLAVSPPAHGEKAAVPSHG
                     VPSVPSVGAEAHAPAPGAAAHGEAAHGAGHGPALPEISPIPGVTFVETMIQLMDHELK
                     GRKLGWRPNDIFFGRFTDNVNNFQLGVLEALRFTTLRLKDSLTRMGDADTYDPDLEAA
                     LNLLMNKATLFWFPSAESSYGDAVDHLRAFVTKLKTGKRSFYYRKDNLVSLLAAYRDL
                     LGNVNKNLIKGDVSWFKTDDYFYYAKGVAHVYYEILRVVRVGFQSQLASTMYALDMMD
                     EVLHELHIAEGLDPWIILDSKLDGFLANHRANLNAPLSEVVHVLVILSQL"
     gene            complement(25479..27314)
                     /locus_tag="PLM79_01585"
     CDS             complement(25479..27314)
                     /locus_tag="PLM79_01585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOV85022.1"
                     /translation="MYNQLLYFVAAILLYSAQQPGSRPLHPPLETFLLCLGVFGTFVF
                     ISILLFRRLARTFRDHSPSSALTAAYHRIQTQLSVLALAALVLYTYVLDIKVYLQRMP
                     WFDQSYTLAGLTGLAVYLLHLLVLWTLSHPFYRRIHQSRIGLPAFLRGHLAFGSAILI
                     PWILISTVSDLVQILGGPGFLQTDPGQFLLLSLVMGLFLLLAPPWVVRLWGCKPIPES
                     PVRAELEEFCRQRKFRVGDLLLWPLFGGEMLTAGIVGILPGRRYILVTQGLLSLLNAE
                     ELKSVVAHEMGHVRRYHMIVYLVFFLLYAFFAFSFNQIILLAVLRHPLVFDLTGSPAA
                     IDHNLFSLLYSLPILFLLVVYIRFVFGYFLRNSERQADLYALRLIGHPFFLISSLEKI
                     AVWSGQIRDLPNWHHYGIGERIRCLVDSYRNPGLIRAHDRKLYRAGAVYLAAILLLSA
                     LGLYWKDSKTVRRWGLEVLTHRFEQEIKRSPHNPMLAASYGGALLELGEYGKAEEVLR
                     RVLALLPENATVLNNLAWLYATAPPPYFHPTASLRLASRAAQLEHEPHILDTLAEAYF
                     VNGDFQSALETIDKALQLHPKNPDYFHRQKKKFEDARDQGERKRG"
     gene            27630..28442
                     /locus_tag="PLM79_01590"
     CDS             27630..28442
                     /locus_tag="PLM79_01590"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOV85023.1"
                     /translation="MILKILAGFAAVYLLVALVVFLFQARLVYFPERDTISTPRSAGL
                     AYEEITLLTEDNLRLQAWFVPAQNSRAVLLFCHGNAGNISHRIDSIALFARLGLSVLI
                     FDYRGYGQSEGTPSEKGTYLDAGAAWRYLVQVRGTAPADIILFGRSMGAAVASWLAVR
                     ENPGGLILESAFTSIPDMAARLYPFLPVRILSRIQYNTLENVQRKRCPLLVVHSLDDE
                     IVPFSHGRRIFSSAQEPKKFLELKGDHNSGFLISGSLYLEGLSNFVAAYLKK"
     gene            28557..29078
                     /locus_tag="PLM79_01595"
     CDS             28557..29078
                     /locus_tag="PLM79_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85024.1"
                     /translation="MKDYPERFARISGRLMGAFLVLVLTAACQTISLDYRSGRVLEQD
                     RIALPDNAEQQGVWKTREAVVEYRFARRGNELKISGVMKFAEPITNNFSGIEHSHLDA
                     IFVNPQGKVLEIRGLMSSAFAHPESPQKFENRLSLPPGTESLAFSYQGRAFSSGLDNN
                     DLYSFWYYPIHPK"
     gene            29228..31522
                     /locus_tag="PLM79_01600"
     CDS             29228..31522
                     /locus_tag="PLM79_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOV85025.1"
                     /translation="MIPSSVIGACLAGFAGTIAQVLVLRELLVLFCGNELSSGLIFFC
                     WLLWTAGGSALAAFRCPKKWDPSSMLVVTLALMAVVFPLTLLGIRGARSLAGIPLGDV
                     VPPGWMLLISMGSTGLFCLTLGALFSLTWAVHVRMSGSREPRPILVYGFEAVGAALAG
                     LLFYYCLLPYFSALCISWMVSSAILCAAWALSLRLEEVASSRTPLFRFGLPGLVALTL
                     AAGFVLEDLDRLSRRWQWGDALVAVEDTPYQNLALVKNEDQLTLFGNGVWLFSVPDPE
                     TEEYAVHLPLLQHPNPRRVLIIGGGTRLVGEVLKHPGIEVLDCVEPDPGVIRLIRKAL
                     GDSPPQPFQDPRVRLHSVDAKAFLTHAPGAYEVILMNVGDPANASMNRFYTVEFFERI
                     GSLLKPEGVFSFSLSAAPEILGPAQARAMASANSTLQAVFSRVTLVLGHGARFLASNP
                     QTFFLLEPEPLAEIIRQRGLTLQYFQAGLLADYLSPLRRAYARSVLEKSGPAQENRDF
                     SPTCYFDHLIVWALQLHPRLGDTFVALSRERNLVLWGLCALGFLVFPVMYRAGRSRRT
                     LPFLGVVALVGAAQMNLQIILLLAFQILAGFLYKDLCLIIAFFMAGTGMGAAASLYLP
                     GGLRSRPRWVFGLQGAVGLYSLGIWGVLWLLHARQLHVQWVPPLSALFCLLSGISGVL
                     GGLHFSLSVEAVSLSKAPSEKTGGGLYGVDLLGGSVGALVGSLLLLPLFGIPATLVLL
                     AVACLGGLILLLPALQRVDGPRPE"
     gene            complement(31542..32702)
                     /gene="amrS"
                     /locus_tag="PLM79_01605"
     CDS             complement(31542..32702)
                     /gene="amrS"
                     /locus_tag="PLM79_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOV85026.1"
                     /translation="MISRRHFLKSCGGCAAFWAGLNLGSPFRTPPSAAAQSMEKGLIR
                     RKLSPYFTPLSKNRIRCDLCPRSCEVEDGERGHCRVRENRGGRYYSLVYGNPCAVRVD
                     PIEKKPFYHVLPASTSFSIATAGCNLNCKFCQNWEISQAQPDDTFNYDLTPEQVVALA
                     RTSQCRSIASTYVEPTIFLEYMLEIGRICQEADILKVMHSNGFIHEAPLRDLCRVLDA
                     ACIDLKGFSEEYYREMTGGSLEPVLATLKRLRELKVHTEIVNLVVPGKNDSPEQVRAM
                     CRWIRAELGPDVPVHFSRFYPLYQLKSLPPTPVATLEQAWKIAGEEGLHFVYIGNIPG
                     HSAEHTQCPQCRTVVIERVGYRVRLTRLDNGKCRNCGTLLPGIWNQREARRT"
     gene            complement(32786..34324)
                     /gene="amrB"
                     /locus_tag="PLM79_01610"
     CDS             complement(32786..34324)
                     /gene="amrB"
                     /locus_tag="PLM79_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOV85027.1"
                     /translation="MQISRRPGCVRFLGNAVLLTALWSCLLGPGDPWAKDSDSDARKI
                     REPVFAGSWYPDSPSELRRLVEGYLERVPAGDSSGRLLGLISPHAGYLFSGATAGYSY
                     RLLRAEKPSTVILIAPSHHMRFSGVAAYPGSGFRTPLGILSVDRAMTDCLGKEAPKIQ
                     LRADAFEKEHSVEVQLPFLQVAAPDCRIVALVMGEQDLETCRWLADALVRCIEKRPAV
                     LVASSDLSHFHPYDQARSLDKVVQERVAALDPEGLSRSLSRNLCEACGGGPMVTAMIA
                     ARAMGGNRAQVLHYANSGDVAGDLNRVVGYMAAAIWADSNVKAGPEPSPSNSPRVGVD
                     LGLSSEDKAQLRKIARERIEAHCAGKKAPHLPSTSPKLAEPRGAFVTLNKHGQLRGCI
                     GRIISDQPLAETVAEMAVAAATRDPRFPPVTSSELKEIEIEISALTPFRKVSSPEEIQ
                     VGTHGLYVRRNGSAGLLLPQVAVEHGWDRQTFLEQTCNKARLPRDAWKDPRTEIYVFS
                     ADVF"
     gene            complement(34623..35522)
                     /locus_tag="PLM79_01615"
     CDS             complement(34623..35522)
                     /locus_tag="PLM79_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOV85028.1"
                     /translation="MANPARAMPGILRNTFCHVPGIGIITERKLWASGILSWEDLLEQ
                     PPSAWGPRAVGVRQHMEASLRSLQEKDIRFFARHLPSFEMWRLFAEFRDAAAYLDIET
                     TGLGSPSDHITTIALYDGTNLTPYLFGHNLGKFRDDVRKYRMIVTYNGKCFDLPFIRS
                     TLGIPMDQVHIDLRYLLKSLGYRGGLKGCERQLGLDRGKLEGVDGSFAVLLWDEYRRR
                     KDPKALETLLAYSLMDAANLEPLMVKAYNLKLELTPFSPDRALPDPATPVLPFEPDAE
                     TLSRIRKRMERLATPWGAFSPVR"
     gene            complement(35654..36172)
                     /locus_tag="PLM79_01620"
     CDS             complement(35654..36172)
                     /locus_tag="PLM79_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85029.1"
                     /translation="MKPSGWLILVLLTLFLLPVWTCAQDKAPAEKTAPQESPAMSHEH
                     HAVPPAGEKESAAVHKTMKVDHGKLVEEWKAADARLQAKLAAMNKAKGEQRISAMMAV
                     INELAAQRQDMRERILRLAHGSKECMMMGKDPGEMHSMHPGKKEKPSCCPMMQKNSES
                     GSGAAKTEESQQ"
     gene            complement(36252..38030)
                     /locus_tag="PLM79_01625"
     CDS             complement(36252..38030)
                     /locus_tag="PLM79_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOV85030.1"
                     /translation="MAVYGVNEFVLDSLPHPVMLIDRKRVVLAANQAAREMGAVVGEP
                     CWRHFAGKEDRAEDPEPGATESRESLSGEGYICRFCLADQAIKENRATQLCQFPLCDR
                     LWNLWWIPVGQSVFLVYAIDVSEEGRARKELEEALHQTTRLQREIMALLAGSKAVLEY
                     RTFEETARALFRICKELLGAGSGYVSLVNERTMCAEVAFLDPGGKECTASEDLPMPIR
                     GLRLRACASRKAIYDNDFSRSEYSLLLPPGHMPLENVLFAPLILREKTVGLLGLADKP
                     GGFNERDVQLASAFGELAAVALVKKRSEEELLRAHQDLEARVAERTAELERANATLRM
                     EIEQRRKAEAALLRSEQQLKFLSSRLLAAQEEERTRLAREIHDQSGQTLAAIKFGIEG
                     AIRMALNDRKGDLIQHLEKLLPTIRAGIAEVRGLYMSLRPTLLDDLGLTAALGWLCRE
                     FTKVYPDIAIAKTFPIDENLVPQSLKIVVFRIVQEALNNIVRHSTATTVELGLKEEED
                     RIHLLIRDNGLGFNLSRTLSSARMRGLGLASMRELSQLSGGTFSIDSVTGSGTTIHAS
                     WPCRRNDPDPDSTFSPTRHDCAEILP"
     gene            complement(38154..38546)
                     /locus_tag="PLM79_01630"
     CDS             complement(38154..38546)
                     /locus_tag="PLM79_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOV85031.1"
                     /translation="MDLILSRDSAKQLERIINNELLDKGVTHAFIVDSSGNLILERGS
                     LPLEDLLPLAALSAANFGASERMAQLIGESDFTLLFHKGTRQNIHFARVSREFILVTL
                     FSNDVPLGLVRLGSGKVTQQALPLLRCG"
     gene            complement(38873..39832)
                     /locus_tag="PLM79_01635"
     CDS             complement(38873..39832)
                     /locus_tag="PLM79_01635"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOV85032.1"
                     /translation="MDLHSVPPSFPIVDGHVDVLFEMMEHHSGRRFSEISSGTVTLDL
                     LKRGNVRIIVSALYCADQYNGEESAAGRFQQLVRYARDHLDGVVPIGSSRELQSVYES
                     PEHEPAVLFLLENADALVDMDLTALDPLRIRVVGLTHMGSNRIGDGNGVFFPEGLRRE
                     GKEVVRKLKDAGCGVDVAHLSDPCFWDLMDLFEGPVLSSHTGFRFFCNIPRNLNRDQV
                     RVLVERRGIIGISVSPDMLALDGVAKAEDVFRHIDWVTQSFGPEFVALGSDFCGFFSA
                     TEGVEDISRLSGLAERMTRDGYPSEAVQGIMGNNWVRFYSALL"
     gene            complement(40021..42054)
                     /locus_tag="PLM79_01640"
     CDS             complement(40021..42054)
                     /locus_tag="PLM79_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV85033.1"
                     /translation="MKIVLVFPPFYLEPMYNLPPLGLLNLAAVLKESPHETLILDFPL
                     AIRQGLVSLGPAVYDHCARRILEEAPDLVGFSVQCTTYPPAIQIARLLKRHDPSMKIV
                     LGGHNASFVDRRTLTAFPFLDAVVRGEGEITFPELVDSYSQGRDGEGIPGVTYRSRES
                     GEVVRNPDRDLLTSLDELPIADYRLVPPLSEYRDACNIRRSIAILEVGRGCPHRCAYC
                     SQSLIWRRRTRTFSVERIIGEIRHLHREFGAECFLLAYDQFTARRSYVEEFCRRMMEE
                     GLNRLPWYCISRLDGVDSTLLRTMRNAGCESMCYGIDSGSKRTLAFIHKDIDESILYQ
                     RVRETTDEGIVPTLSFVIGFPREEVEDLDATLVLALKSGGVGNTNPLIQMATILPGTE
                     LYEHYSHLLVREVDTYFSLGIEFDGGRRLEGDEDLIDSDPLLFSSFYNLPCPAGSLRD
                     LNLLATYFPLMVNYFPRSFLLLVHELEESASHLFLHWMLRVQQRRGGNEPSLSPEDCF
                     RYFGDYCRERLGQRERIRFAHLPDVIEYERTSMEAGKYESLPGEFSMDIHGIRLPGPQ
                     RDRRVLMKEFTFPMTDIIMDLKNGEVGSFYAPRETFLVFRKVEDRLDVKEINRFGRDL
                     IAWCDGTNSPTEICERLYPVHGSSHSRDRFMVLCVDAIRTLEQMEILRHGSSE"
     gene            complement(42189..43175)
                     /locus_tag="PLM79_01645"
     CDS             complement(42189..43175)
                     /locus_tag="PLM79_01645"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HOV85034.1"
                     /translation="MIQITNSMNQFMKDHPRMKYIFFGGKGGVGKTVMAGAAALWSAR
                     QGRKTLLASTNPVHSLSNLFGTNVFGKPTVVCDESNCYAFEIDTRDTIERSKREIREK
                     INWFLKFADITTKADEFIESATMNPAFEESAMFENMMDLMFRDEYEFYIFDTAPTANA
                     RRLLGMSKVYSLWVEKMLRSREEAKSLREMLSFSKKKEEKDPLLEYLLGFKDRMAHAK
                     DLLTDEALTAFFFVTLPESLPIAVITRFIHWFHEFGIPVGGVVVNGVIQKDQVGGDAA
                     DFVLNRIRMQEEHLARIAETFGDQVRAVTPLFETEIKGTPMLQRLVDSVFGE"
     gene            complement(43195..44124)
                     /locus_tag="PLM79_01650"
     CDS             complement(43195..44124)
                     /locus_tag="PLM79_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HOV85035.1"
                     /translation="MFGGKGGLGKTTFSAATAYYLAKTGKRVLVFSVDPQASLSDIFQ
                     RDIFGKGPVEIMPNLYAQEIDADRRVKEYQEEIRHKILDMYGMEQIPDEIESYIQAAA
                     AEPAMEESAIFDEVVDIVVKGGYDYYIYDLVPLGHALYYLSMASVYDEWIDKITKLRE
                     QMSEYDHVAAVIRRDKESEEDAILKELLYIKERINKSSSILTDKEKTAFFFVLVAEEM
                     VIKDTQKAAELFAKFDVPLSGYIINRVLPSELKEKNIPEYLKNRFVMQEHYLKVIETD
                     FSGQILASVHEMERDVTGLAMIEKMARSMYGDF"
     gene            complement(44285..44560)
                     /locus_tag="PLM79_01655"
     CDS             complement(44285..44560)
                     /locus_tag="PLM79_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85036.1"
                     /translation="MSKDEKGKKGGLFQAVKEFVYGVAAHDSARFALKTRASMEHLFI
                     LITMGDMLGVPILPPYYSLRLLPYVVPQISTWKRRMLREKDLTDALA"
     gene            complement(44930..46609)
                     /locus_tag="PLM79_01660"
     CDS             complement(44930..46609)
                     /locus_tag="PLM79_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOV85037.1"
                     /translation="MNSVLVLVLAFLVFFIGYRFYSRYVDTNIIKADPKRATPAVMYM
                     DGVEFMPTSKNVLFGYQFKSIAGAGPIVGPIIAIQWGWLPALVWILVGTLLIGWVHDY
                     ASTMVAMRNEGASFGGLSYRLISPRARIILLSFIYFYLLLITSAFGNVVVSTAVNLKA
                     SPMAWLFLTIGGILAGQMIYRWRKDIILTTVVTVLIAMVGIWMGTVAPSDKILGEALS
                     NNRALWAIAAFVFCYFAAVLPIWRFALPINYVASYIVFFGLLGGVIGVLILHPDFTLP
                     AYTSFTIKIGPIWPIMFVTIACGAISGWHSIVSSSGTARQLENEMDARPVGAGVMFVE
                     MMLAVFALIIAGTIYASASEYGAAVAKGPAGVFAAGVSKFLSALGISAGIGKSYGSVM
                     MIFLAITIMQLAVRFMRVATSELLGDVSPLFKNPHIGTIVASLLGLVLVMTGWWQYLW
                     VLFGGANQLMASLALMLITAWLMSEGRSSAWTFYPMIFMFVTTVGALLYTSYSLLDKV
                     FRGVVKGEALIGNTIMGIVGFFLVIAAIILAVEAFKAFGRYRANRMQPASA"
     gene            47412..47897
                     /locus_tag="PLM79_01665"
     CDS             47412..47897
                     /locus_tag="PLM79_01665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOV85038.1"
                     /translation="MTTKQWDSPPSMGIDSSKLYRAKLETNKGVILLELYPQYAPKTT
                     NNFVFLCREGFYDGVIFHRVIEDFVIQGGDPTGTGRGGPGYRFEDETRGNPLKHETGV
                     ISMANAGPNTNGSQFFITHSPQPHLDGRHTVFGKVMEGMEVVKAVEQGDRILKAEIEE
                     A"
     gene            complement(48259..49413)
                     /gene="nrfD"
                     /locus_tag="PLM79_01670"
     CDS             complement(48259..49413)
                     /gene="nrfD"
                     /locus_tag="PLM79_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOV85039.1"
                     /translation="MLERAFKGDQRYFRWLMLLLVLMGMGFASYLVQLNRGLTITGLS
                     RDVSWGFYIAQFTFLVGVAASAVMVVLPYYLHNVKVFGRITILGEFLAVASVTMCLLF
                     IIVDLGKPMRLLNVLLYPTPNSILFWDMIVLNGYLFLNIIIGWNVLEAERKGVHYPGW
                     VKPLIYLSIPWAVSIHTVTAFLYAGLPGRHYWLTAIMAARFLASAFCSGPALLILLAY
                     LVKKYTKFDPGKEAIDKLSIIATYAMVISVFFVGLEFFTAFYSKIPSHMEALKYLFVG
                     LEGHRTLVPFMWLFAILAVVALVLLINPNTRKNEKTLKIACAAVFLSMWLEKGMGLVI
                     AGFIPNPFDHVREYSPTIPELMIVLGVWATGFFILSVLYKIATTIKEETA"
     gene            complement(49428..50210)
                     /locus_tag="PLM79_01675"
     CDS             complement(49428..50210)
                     /locus_tag="PLM79_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV85040.1"
                     /translation="METNRRSFLKIGGLCTLGLATLPIHELLAKTDLPRVASNPEAFH
                     GKQWAMVVDMRKCWEKGKAGCKDCIDACHKVHNVPEMGNVKEEIKWLWTDNYENVFPG
                     QGNPYNIENLRGKPFLALCNHCANPPCVRVCPTQATFKRADGITMMDMHRCIGCRFCM
                     AACPFGARSFNFREPRPYIPELNMAYPTRERGVVEKCEFCSERLEVGLMPACVEACKV
                     GGLAFGDLADPKSNVRKLLASNYTIRRKPELGTEPNVYYIVS"
     gene            complement(50210..50623)
                     /gene="dsrJ"
                     /locus_tag="PLM79_01680"
     CDS             complement(50210..50623)
                     /gene="dsrJ"
                     /locus_tag="PLM79_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HOV85041.1"
                     /translation="MRIYNAKTIIPGIIIFLGIATFPLWYNMGKAAPAPDPKIDTPVI
                     QQMAVKQCVLPKIEMRTGHMQLLNDWRTSVVRDGFRIYVAADGKQYNMSLQNECVRCH
                     SNKSQFCDQCHVYAGLELNVTPYCWTCHIAPKETK"
     gene            complement(50610..52229)
                     /locus_tag="PLM79_01685"
     CDS             complement(50610..52229)
                     /locus_tag="PLM79_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85042.1"
                     /translation="MAKVPKPNELVIDHKLPQTGWMDTPVVFRPGTFSYPGKPKNLEV
                     VGLPNPREWSPMDEDWKLPDNWKEIILEGLRERLERFRSLRVFMDICVRCGACADKCH
                     FYIGSGDPKNMPVLRAELLRSVYRKDFTTAGKVLGRIAGARELTVDVLKEWFYYFFQC
                     TECRRCSLFCPYGIDTAEITIIGRELLNLVGLNIDWVVGPVANCYMNGNHLGIQPHAF
                     KDMIEFFVDEIEAVSGVRVEPTFNRKGAEILFITPSGDVFADPGTYTLMGYLMLFHEL
                     GLDYTWSTYASEGGNFGFFTSHEMMKRLNAKMYAEAKRLGVKWILGGECGHMWRVINQ
                     YMDTMNGPADFLEEPVSPITGTRFDNAKSTKMVHIVEFTADLIRNGKLKLDPSRNDHL
                     RVTYHDSCNPSRGMGFFEEPRYVIQSVCNNFFEMPPETTREQTFCCGSGSGLNNDEFM
                     EMRMRGGLPRANAARYVQEKHDVNMMACICAIDRAVLVTSLNYWAPGVGVTGVHELVG
                     NALVMKGEAERTTDLRGEPLAGMEESDDEDL"
     gene            complement(52232..53224)
                     /gene="dsrM"
                     /locus_tag="PLM79_01690"
     CDS             complement(52232..53224)
                     /gene="dsrM"
                     /locus_tag="PLM79_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HOV85043.1"
                     /translation="MGIKFSLTVVIALVLAAFLGVQAAGMTYLFGVIIPYLAVFIFLL
                     GVVYRVITWARSPVPFRIPTTAGQQKSLPWVKQNKLDNPSSTAGVVGRMLLEVLLFRS
                     LFRNTKFELHQGPKVVYQWEKWLWLAGLLFHWCFFVILLRHLRFFTEPVPFFVLMTDR
                     LDGMLQLGLPGVYLTDAIFILAVTYLFIRRVIIPQVRYISLPADYFPLFVLLGIGITG
                     ILMRYFLKVDVVRVKELAMSLVTLQPKVPDGIGVLFFIHLFLFSTLFAYFPFSKLMHL
                     GGVFLSPTRNLPNDSRMKRHVNPWNYPVKVHTYEEYEDHFREKMIEAGLPVEKE"
     gene            complement(53258..53803)
                     /locus_tag="PLM79_01695"
     CDS             complement(53258..53803)
                     /locus_tag="PLM79_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HOV85044.1"
                     /translation="MNLGDLLARDRERFVKKWFGALVGTYPPETARFLGKESNQFVNP
                     VGHTIYHGLDGLYGEMLRGMDSEKLRPWLDRIIRVRAVQDFSPSQAVAFMFLPKGLVR
                     DMFQEHIQTQQVSSEELASFDLLIDQISLLAFDVYMQCREKLYELKVNELKNRTFRLL
                     QLAGLAAEIPEFRAEGPKGNT"
     gene            54331..54963
                     /locus_tag="PLM79_01700"
     CDS             54331..54963
                     /locus_tag="PLM79_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOV85045.1"
                     /translation="MAKYRGIHHLAMATNNMDSTIRFWRDLLGMRIVAGLGKPGYRHY
                     FFEISSEDLIAFFEWPHVEPIPEKDHGYPVSGPLVFDHVSIGVETEEDLWELKDMLEA
                     AGFWVSEVIDHGFIHSVYSFDPNGIPIEFSCTVPGVDVRRRPVMVDSAPSEVTLEGPE
                     PRPQWRPPVTEPTAEVDRRVYPGEGVDLVEGKKKNWWEDRVHLPGPQPQP"
     gene            complement(55005..55292)
                     /locus_tag="PLM79_01705"
     CDS             complement(55005..55292)
                     /locus_tag="PLM79_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85046.1"
                     /translation="MEWTKTKPKKEGWYWYLYTGNPEDWPMVDDPSQPLIVEVTRQFF
                     IDEDLSLLLFWLTGSDEPWRVYEAEGFWYGPLLPPPFPEILQHTDGGNGGT"
     gene            55909..56139
                     /locus_tag="PLM79_01710"
     CDS             55909..56139
                     /locus_tag="PLM79_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85047.1"
                     /translation="MKVRLMTRETARYVESGQIRGEVKRNKYITATRITDTGENLAKI
                     LKTTDHGSIFELFLEGRRVPWTPPEASNPPKP"
     gene            complement(56281..56356)
                     /locus_tag="PLM79_01715"
     tRNA            complement(56281..56356)
                     /locus_tag="PLM79_01715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56321..56323),aa:Ala,seq:ggc)
     gene            56861..56948
                     /locus_tag="PLM79_01720"
     tRNA            56861..56948
                     /locus_tag="PLM79_01720"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56895..56897,aa:Leu,seq:caa)
     gene            57831..58148
                     /locus_tag="PLM79_01725"
     CDS             57831..58148
                     /locus_tag="PLM79_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85048.1"
                     /translation="MLTRREMARICREIGCKKVLFDSRDLELDGGYTTRSLYELGPTY
                     RCDGIGLQISIAHVMPRHAGVRREIRSLTTVAKNRGTWVESFEDVSEAEKWLRGPRNG
                     KGL"
     gene            58389..59045
                     /gene="elbB"
                     /locus_tag="PLM79_01730"
     CDS             58389..59045
                     /gene="elbB"
                     /locus_tag="PLM79_01730"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOV85049.1"
                     /translation="MPKIGVLLSGCGVYDGSEIHEAVLTLLALDRANAQIVCMAPNVD
                     QAHVINHLTGEVTGEKRNVLVESARIARGEIRDLKDVKAADLDGLIIPGGYGAAKNLS
                     DFAFKGPDAVVNPEVERLLREMVAANKPVGAICIAPATLTKALADKKPEVTIGTDKDT
                     AAAIEAMGGQHKVCSVDQIHLDAGNKIVSTPAYMLGPGIKDVAEGIGKLVEKVVALAK
                     "
     gene            complement(59126..60133)
                     /locus_tag="PLM79_01735"
     CDS             complement(59126..60133)
                     /locus_tag="PLM79_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85050.1"
                     /translation="MTLLRLFSQRSCLPRPLGPVLQLTIAFFLILCGPRAVPAADNPK
                     PPSSPVKLIFIHHSSGENWLADCNGRLGIALRNSNYFVSDTNYGWGPRDLDVGFDRIG
                     DHTDLGHWYNWFAGPNRSRYTKALYAESGQHCDYSRLSQDPGGPNEIILFKSCFPNSN
                     LGGKPTAKPPAGENPLRGQDAYSPYMKVGYAKGIYNDILQYFETRRDKLFVVITAPPL
                     NPNETSAAQAANARAFNLWLVNEWLKDYPHSNVGVFDFYNVLTSNGGDPRTTDLGKAR
                     GNHHRWWAGALQHIHTVNRNVAAYPSGSDDSHPNRVGNRKATGEFVKVLNVLYHRWKA
                     D"
     gene            60318..60446
                     /locus_tag="PLM79_01740"
     CDS             60318..60446
                     /locus_tag="PLM79_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85051.1"
                     /translation="MPIPRQREQGTVPRPEQAGHDRLEPGRLGTDVIRHFLEQLRI"
     gene            complement(60659..60847)
                     /locus_tag="PLM79_01745"
     CDS             complement(60659..60847)
                     /locus_tag="PLM79_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85052.1"
                     /translation="MSISRRILLGLIVGATGFFQIADNPMPGISAVCLGGFLILAGID
                     PLNRSKNAEEAMESDGRR"
     gene            61216..61818
                     /locus_tag="PLM79_01750"
     CDS             61216..61818
                     /locus_tag="PLM79_01750"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV85053.1"
                     /translation="MATKGKLTRQTIIEKSIQLFSVKGYFHTSIADILEAVGITRGGL
                     YGHFRSKEEIWYAAYQECTRIWKDIVFRGVKEAPDPLERIRRVIRNSMQDYLGGGVFE
                     GGCFLFNSLVEFSRQSSAMNQHILQGFRGFSRLLHSWLEEARQMAMLKEGLPLEEIAN
                     FIVISLNGAGPLYTSSGDPAVWQQTISQLHQYLDHLKKPT"
     gene            61971..62747
                     /locus_tag="PLM79_01755"
     CDS             61971..62747
                     /locus_tag="PLM79_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOV85054.1"
                     /translation="MSYTNVLVEKKDRIGFITLNRPQEMNTFNIPFARELNDALWEME
                     NDPEVRVVVINAAGKHFSTGISLAEFKGKSHKEYREFIRLMDEHNHTIAKMKKPVIAS
                     VKGLCLANGAGLSFACDLTVATEDSKFGTTAINVGLICLGPAVPLARCVGRKKTLEMV
                     LTGKMITAAEAERLGLVNRVVPKEQLEEATLELATQLAAKSPLALQVGKTGIYAMQDV
                     PYNQALDHLSLLFASHCSTEDAEEGVRAFLEKREPVWRER"
     gene            63261..63521
                     /locus_tag="PLM79_01760"
     CDS             63261..63521
                     /locus_tag="PLM79_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85055.1"
                     /translation="MNVEEVMEKAGFRLSASCAGQASYTKFVQHKGKRAYVTVTDGSG
                     EGFPRTLDEPVQVGTFDLRSGDELTPVQGFSSLREYLESIRE"
BASE COUNT        13422 a        18393 c        18888 g        12932 t
ORIGIN      
        1 acctgaccgg aaggcccgac tcgatgctct gcttcaaacc gaccggcctc tcttcactat
       61 cggggcgggg gatgaagccg ctctattcct catcttccat ctcgactccc agccgttccc
      121 actgagcctt ggcttcctct tcagcccttg caacctcctc atcgctccag ggctcgatca
      181 ccaggctatc cgccacgagc tcccagaggt atttcacctc gtatttgcag tcctcggggt
      241 gatatttcgg aagccgcttc atgagctggt cgtggcagtt ggaacagccc acaacaacga
      301 cattcgcgcc ggcatctttg atctgtctca ccttgtgacg cccgtaatag gcggattctt
      361 tttcataggg catgggccag ttcccgccgc cggcaccgca gcagtaattg ttcatgcgag
      421 tgggctgcat atcgacgaag tgatccacac actgctcgat gatccatcgg ggttcgtcaa
      481 agtagccctt gccgaagttc cgctcgagct cccggccgtg tttgcaggag tcatgccagg
      541 taaaaacctt gcctgcatgg acgctcttat cgagcttgat tcttcccgtc tcgatgatct
      601 ctttgaggta gtcgtaaaga tagatgtggt tgatctggtt gttgggatct tccaggacgc
      661 actctttcat gcccttgcgg cacccgtagg agccgccgcc gcagtcgggc atgatcatgc
      721 ggtctatgcg gtgctgtttc atgaactgga tctttctttg agccaggatc tttgtggcct
      781 cgtaatttcc ggtgaaaagc ccccagtcca ccgcctccca gttttcggag ggaatggtcc
      841 agtcttcctt tgccgcatag aagattttcc accaccaaat catgtcgtcg ttatcggaaa
      901 agacctcttt ggagttgggg aagaagagaa tcttggcctc gtctttgtcg atggggacat
      961 agaagccggg gagctcgcct gcaagctccc gacccatatc cgacatgagg aagaagtagt
     1021 cttccttggg gatggccatg ttgttcccgg tattcagaac gttctcgacc cctttatgga
     1081 ggattcccgg gacctggtcc cgaggtcgaa gatgcttcat gtggcccatg atgcccacga
     1141 tatcgattcc catggggcag gcatggacgc agcgaccgca gccggtgcag agccagggga
     1201 atttcgactg caccacctcg tccaccatgc cgtaggcaag catcctgagc accttacgcg
     1261 cgtccagccc ttccgcgcct tcgcctcccg tcaccggaca tccattgatg catgtgccgc
     1321 aggtgaggca caggctcagg ttgaacttgc tcagatagtc cttgacatcg tttttcaaca
     1381 tcgggttgag tactgcttgc tccatggctc catcctttcg cagaagttgt cgaccttaaa
     1441 tcattccaaa atattcagcg ggctcgtctc ccggaacggt attcatgagc tcgcgcacca
     1501 gacgcggggt cgagaggagg gccgcttcct cgcgaagatc gaggacccac caatcacagg
     1561 aaaggctgct caggagcctc agccgatcca tcccgtcgtc cggctgttcg ggaggcagtg
     1621 tccgtcgtcc cgttccacgg ggtagatgtg ggcgttaaat actccttcgt gatgcggaga
     1681 aacgaagtcg tcgaagttgt aatgtccggt cggcatcgaa gggcttatgc gggcatggtg
     1741 atgtccaggg tgacacctgc cctggatttg cggatcaact tttcaaagag ctcgggaggg
     1801 ctcgaccatc ccgaggccgg attttccaag cagaggcaaa tcccaaagtc gtccgcgtac
     1861 tccaccagct ccgtcatggc ttcgataccg cgctcccagg aaagctcccc cgtggaacga
     1921 gaccccaagc caaggtgaac cgtcatgaat cggtcctcca gcctggaaac catgcggcac
     1981 accttgcgat gggtctccat ggccgcgctc gccccggctt catcggcatc gccgaaatcg
     2041 attcccttga aggcacggtg aacctcgagt ggatgaagcc gggagatttt cccgagcagc
     2101 ctggattcat cgagaccgtt tgtgggaagg tcctcaggtt tgaacgtcca gtccacgccg
     2161 gaaaacccgt gagccatggc cgccttcttc aacacatcca catcgggtat gaaattacac
     2221 atcgccggct tcggttgact cacacatcac cctgtccccc agtatgtttg aagtcttttt
     2281 ctttctttgt atccgggaat acgaaaaacg tatctcttct catatcaacg gcctttgtaa
     2341 agtgagcttg tcaattgatc gaatgggtaa gcccaatcca gttccgccga tgcacaaaaa
     2401 gaactattga atcggttgtc gatcattccc gaacttcgcc gggtgaagct actgcgggcc
     2461 ctgagggagc agaagctctt cacccaagaa cgtgaacggg gacatggctc cttcggtacc
     2521 gggtgtgtag ccgtagattt cttccaccag gcgattgact tccttgtaga gggaccgaag
     2581 cggaatgcgg gcaggacaca cctcctcgca aaggccgcag tcgatgcagc gccctcccat
     2641 atgtactgct cgaaccaggt gaaaggaggt atcgggcgga agctcggtgc cgggaatgag
     2701 atccgggtgc tgcaggctgc attccgtgca aaagcagact gggcagacat cgcggcatcc
     2761 atggcagcgg atgcaccggt tcatatgagt gcgccaccag tcgagcctct catccatttc
     2821 catcgcgtgc aggccctcta ttctgtcgtt tttggaaacg ggggcaactg cccggccgta
     2881 atcgagcctg tccggatagg gctgccggca ttcgcaataa agggccatct cttcactgca
     2941 tgcctgcccc agggcgatca ccttattcct gtcgagttgg ctccatttga agagctcttg
     3001 cagaccgcgc tcttcgcagc ctcgcaccag gataccgtag atcttctccg ggttttggcg
     3061 cgagagagcc aaaaggagct tcaagagcgg atagcgtgcg gaggtcggct gccagggctc
     3121 cagttcatgg gcgttttcgt tcgtaaagag ccagggaacg ggaaatccgt gagccgtctt
     3181 gtacgcccaa aacccatcta cccggccttc ctccaaaagg ttcttgattt tcgtttccat
     3241 ctcaacccaa tccttcacga aagaagaaga caggagacgg aataaagacc gcatcctatg
     3301 cagtccggct tccgacttca gtctcctgtc ttccatattg aggaatcgcc tcgaaagagg
     3361 gtgtggggcc aagggtttga atccgggagg taaagtctcg gaccacttcg gcaaacttga
     3421 tgccttcagc agccgagatc cactccagac gaagcctcgc cggatcatag ccggcaaaac
     3481 gcagcaagcc ctcgatgaac ttgacccgct tgagagtcat gtaattgccc ttgagatagt
     3541 ggcaatcccc gatatgacag cccgccacga gcactccgtc ggctcccttc tgaaaggcgt
     3601 gcagcaggtg atggagggag acgcttcccg agcacatgac ccggatgacg cggatattgg
     3661 ggggatactg caagcggctc actcccgcca ggtccgcccc ggcgtagctg caccagttgc
     3721 aaagaaaagc caggattttc ggctcaaatt ctctttctct catgtggaga cctcatttcc
     3781 tcattcctat gccgaaagcg cttcatcgat ctggcgataa agttgaagat ggctgaatcc
     3841 catgagggaa accgcctccg acggacaggt cgcggcacag gtcccgcagc ctttacacag
     3901 ggcctggttc acttcgcact tggcttcccc ttcgatggtg ccgatagcgc cgaagggaca
     3961 gcactccacg caggcgcggc atcccgagca cagctccttt tgaactacgg cggccacgcc
     4021 tcccgccgcg atgaagtccc tggcaagaac cgtggaggcc ctcgatgccg cggcctttgc
     4081 ttgggcgatg cactcctccg tgggcttggg ccaatgggcg agacctgcga cgaaaacgcc
     4141 ttccgtagca aagtccaccg ggcgaagctt catatgcgct tccaaaaaga agccctcggg
     4201 gttaagcgga accttgaaca tctttgccgg cgcatccact tcacgagtct cgattgcgct
     4261 cgccagacac aggatatcgg gcttgatgac tacgcttcgc ctcagaatcg gatcgaatac
     4321 cgacacccgc ggggtgtatc cttcgatctc gacccgtggt ttccggtccg gatcgaaacg
     4381 aacgaagata acacccttct gtcgggcttc cttgtaggtg ttttcccgaa gcccgtaggt
     4441 gcgaatatcc cggtagagaa catagacgtc catatccgga ttgatcgcct tgagccgaac
     4501 ggcgtttcca atggagtggc tgcaacacac ccggctgcaa tagggacgat ccggctccct
     4561 tgatcccacg cactggatga agacggccga gcgggcgctg acgaccgcct gatcccggtc
     4621 ccgaatggct tcatccagct cgaggttcag gaagaccttg gggctttttc cgtacgcgta
     4681 ttcagacggt ttgtaggaat gggcccccac ggcaatgacg gccacgccgt ggtgcactgc
     4741 ctcctctttc cctcccacat ccaaaacgct cacgaagttg cccacgaacc ctttgacttc
     4801 tttgagcacg gctccctttc gaactgttat gagaggatga gtctcgacct tttcaatgag
     4861 cgcatccaca tagggacgca cgggctcgcc gttccacacg gtgaggagct tgagtgcgtg
     4921 tcctccgagc tctttcttct tttcaaccag ggtcacggga taccccaggc ccgcaagagc
     4981 cgcggccgcc tccatgcccg caacgccgcc gcctaggacc aggccgcttt tggtaatgtt
     5041 gagccggagc tcttcaagag gctgctgaag cgagaccctg gcaacggcca tgcgcaccag
     5101 gtctttcgcc ttggctgtgg ctgcttccgg aaaggcctgg tgtacccagg agttctggtc
     5161 ccttatgttg gcgagctcaa aaaggtattt gttgagcccg gcttctcgaa tggtttgctg
     5221 aaaaaggggc tcgtgagttc gtggagagca ggaagccact acgatgcggt tgagcttgtg
     5281 ctcgcgaatg gcttccttca ttcgctcctg cgtatcctga gagcaggcaa aaaggctttc
     5341 ctccacgtag gcgaccccgg aaagactttt ggcatactcc ttcacgtccg gaacgttcac
     5401 aaccgagcct atgttgattc cgcagtggca aacaaaaact ccgatgcggg ggggatagag
     5461 gtttgcccca tccatttctt tcggttcttc gggcttgcgt gcaacggttc cccgaaccgg
     5521 cgcaagaaga gccgccgttt cagcagccgc agccgatgct tccatgacgg actggggaat
     5581 gtctttgggt ccctgaaagg caccgcaaac aaaaattccg ggccgtgtcg cgacgactgg
     5641 tgcaaagggc gaggtcgagg caaaccgacc ctcattgacc tcgatcccca acctgcgggc
     5701 cagatccatg acttccggcg atgtctccaa ccccacggaa agcaccacca ggtcgaactc
     5761 ttcttgccgc accaccccat cgtccagggt gtagcggagg ttcagattct ccgatcccgt
     5821 taccctctcg acggaatgga tgcgggagcg aacgaaacga actccgtgct cttcacgagc
     5881 cctgtcgtag tatttctcga agtccttgcc cgaggtcctc atgtccatga agaagatagc
     5941 cgtatcgagc ggctcgcggc tatgctcctt ggcaattacg gcttccttga cggcgaccat
     6001 gcagcagacg gcggaacagt ggccgtggtc gcagcggttc atctcccgcg atcccacgca
     6061 ctgaagccat gcgatgcggt gcggctcctt cctgtcggag gggcgaacga gctgcccctg
     6121 gtaggggccc gacggggaga gtatccgctc gaattccatc gaggtgatta cattcgggaa
     6181 gtgcgcgtag ttgtatgtat cgaagatctt gggatcgaaa gcttgaaacc ccggagccag
     6241 gatgatgctt ccgaccttcc gggtgaaatc cctgtcctta tcgtcgaatt taaccgcacc
     6301 cgcggggcag aacttttcgc acgcccggca cttgcccttt tcaaaataga tgcagttatc
     6361 cttatcgatt gcgtacttga ggggcaccgc ctggggatac ttgacatagg ccgctttacg
     6421 ccggctcaaa cccaggttgt aggaatccgc caccttcctg ggacatttct ccgcacacag
     6481 gccgcaggcg atgcattttc ccatatcgat gtagcgagcc ctctggtgga ggctcaccgt
     6541 gaaattgccc gcctccccca gcacctcact tacatcggtc agagtgagga tttcgatatt
     6601 gggatgccgc ccgcattcga cgagttttgg cgaaaggatg cacatggagc agtcgttggt
     6661 cggaaaggtc ttgtcgaggc cgctcataac accgcctatg gcttcgctct tttcgaccat
     6721 agtgacaaag tagccggtat cggcaagatc cagggcggcc tgcacaccgg caatgccgct
     6781 tccgatcacc atggccgatc cgatcatgtt ctcagatgat ttagacatat tcacaccttc
     6841 ttctaccggt ttaagtattc gtgcatggcc gatgccgcgc gcctgccttc tcccatggcg
     6901 gatatcaccg tggcgccacc ggaaacgatg tcgcctcccg cccatacgcg ggccttggag
     6961 gtctttcccg acttatcggt caccaccgtt ccccgtctcg atgtttcaag gtccggagtg
     7021 gtagaggcga tcaggggatt gggagatgtt ccgagggaga ccaccaccgt atcgacgtcc
     7081 atgatgtatt cgcttccggg aatgggcacg gggcgcccgc gcccgctggc atccggctca
     7141 ccgagcttca tgcggatgag ttccatggcg gagacgttgt ttcgtccatc actgatgaac
     7201 gccgtcggat tgcacaggaa atgaaactcg atgccttcct cttcggcgtt ttccacttcc
     7261 tcgagcctcg cgggcatctc ctggcgggtg cgccggtaga tgacgtagac ttcgctcgct
     7321 ccgagcctta ccgcgcaccg ggccgaatcc attgcgacgt tgccgcctcc gatcaccgcc
     7381 actcgctttc ccatcttcac gggagtggcg taatcgggat agaggtaggc cttcatgaga
     7441 ttggtgcggg tgaggaactc attggccgag tagaccatat tgaggttttc tccgggaata
     7501 cgcataaact ggggaagccc ggcgccggtg gcaaggtata cggcctgata gcgtgagttg
     7561 agcagctcat cgaggctgta gttgatgccg attaccgtat ccagctggat gtctacgcca
     7621 agggacgcaa cgtattggac ctcccgttgt acgatttcct tgggcagccg aaattccgga
     7681 atgccgtaca tgagaacgcc tcccgccaca tggagcgcct cgaacatcgt gacctcatgg
     7741 cctcgaacgg caagatcgga tgcaaccgtg agccccgagg ggcccgttcc gaccacggcc
     7801 acgctcttcc cagtggatgg agcgatctcg ggagtcgtaa cagctccctg gacccggtcc
     7861 cagtcggcta tgaaacgctc caggcgtccg atggcaaccg gagccccttt tttgttgagc
     7921 gtacacgagc tctcacactg cagttcctgg gggcagacgc gaccacatat gcccggcaga
     7981 gacgtcttgg tcctgagtac cttcgaggct ccctcgatat ttccttcctg aagcaaccgg
     8041 atgaaatccg gaatatcgat attgacggga cacccctgcc ggcagtttct cttcttgcat
     8101 tggatgcagc gcccggcctc ttccatggcc atctcataag tgtagcccgt tgcgacctca
     8161 tcgaagttgc gcctgcgcac ctccggctcc tgcttgggca ttcccacacg gttgaggttc
     8221 atcttggatt tcatgctctc ggcctctttg ggaggcagcc cggtctcttt tgcttcactc
     8281 atcgttcaac tctcttcact gttcaccgct caccgttcta ccactgggaa cagctgcggc
     8341 attgaaagct ttgctggagg atgctctcgt gggcatacgt gcagcgccgc gccatgagca
     8401 catcccatcc gtccacttca tggccgtcga attcgggacc gtgcacgcag gcaaaccggt
     8461 tgctcccctt gacgctaaga cggcaggctc cgcacatccc cgtaccgtcc accataagcg
     8521 gggtgagatg cacgatcgtc ttgatggcgt aaggccgagt caattcgcta atcactcgca
     8581 taaggcagat ggagcccatc acgatcaccc ggtcggtagg ggtctgggga tgatcggcga
     8641 gaagcctcct caagggtcgg gtcgtgctgg ttgccatgtc ttctccgttc gtgccgacgg
     8701 ccaggatcac tcggtcgctc acagcctcca actccgcttt tccgaaaagc cgctctcggt
     8761 ccggagcctg caggatggtg gtgatacggt tccccttttc tttgtaggca tcgagaatgg
     8821 gagccgtggc ggcgatcccg tatccgctca caaccgccac cacgtggccg aagtgatcca
     8881 catgggcggg cttgccgaga ggccccacga aatgagccag atggtcccct gccttcagcg
     8941 ccgcaagcgc tctcgtagtc tttccaacct cgatataaac gaaatcgact gtcccggctt
     9001 ccctgctcca tccgctgatg gtgaaaggaa cgcgctcacc cttttcatcg acaaggatga
     9061 tcacgaactg gccgggcctg gctttttccg caacgaccgg cgcctgcacg gtcattcgat
     9121 ggaccatggg cgcgagctct tcatgtgcaa cgatggaaaa cattgaagtc acctcattca
     9181 cctgtttttt ccacacggac ggagacgatc ctcaccggcg aatccaattc ctttttcagc
     9241 gatgcaagca acgccatggg agccgccacc actgtcggcg gcaggagcgg gttcacgcca
     9301 aggcgtccgg cttgctccaa tccttcatgc gtcaccacgc ggacggtatc tccgggcatg
     9361 agccccatgc caaagaggtc cgaagggttc atctcgactt cacagtcggg gtagaagact
     9421 gtcttggctt ctggggccag gagcggtcca aggaaataag gctccaggtc ctccttggcg
     9481 acggcggcaa aagggacacc attgctcctg aagccttcgg gggtccagag gggcacgggc
     9541 ttccattccg gcatatcgtt ttgacaatcc gaggggagct tcagcttcac gggcgtcccg
     9601 cagcaactgc accccgacga cactttctgc gaccttcgaa ccagggtggt gctcgccgcg
     9661 atttctttcc agatctcatc gcgtgccgct tcttcatcct ggctgccaaa gcccgggact
     9721 ttcatacgtt gagcgagctc acgcaggagc cacaaaacgg acctggcttc accctgtgga
     9781 gagacaacgg gtccgatgtc gatctgtttt cgttccgagg ttgtgaaggt cccacctttt
     9841 tccatgaccg aagccatggg caggactaca tggggccaac ggccttgttc cctactctgc
     9901 gaagggggaa ggaccgtatc ctggaggacc accagttcga ggctatcgag aaaaggccgc
     9961 agggaatcaa aaaggctgtt ctcgtaggtt tcgaaggcca ggtagaccgc cttgatctgc
    10021 gatgaagcga gcatttcgaa gacatctccg gacggtggaa caccgcagcc ggaaccttgc
    10081 cctcgagctt cgaacagttg ggaaaccagg ccgatatccg aagccccttg aaggtttgcg
    10141 tttccgtagg gccgaatcat gccgccgccg gctttggata cgcttccggt gagatgaagg
    10201 agagtaacca tagcgtcgat cgattcccga accgaaggag attgcaccag tccgagtccc
    10261 aagaggatcg taagcggctt tcttttgccc aggagagatg cggcatccgt aagagtctcg
    10321 aggctcacac cggtgattcc ggctaccgct tccggctcgt aacttgcgag gctcttctga
    10381 agggtggaga cggcctgggg attccgggcc acaacggccg ggtcttcgtg gttttcatcc
    10441 agcaggaagc ggatgagtcc gccgatgagc acggcttccg tcccggggag gtaatggagg
    10501 tttacgtcgg catagcggct gagccctatg gaaagaggat tcgccacgat gagctttgtt
    10561 ccattaagag tggcttcccg aagggccgtt ccggcgatgg gatggctggc cgggggattc
    10621 actccgatgg caagcacggt gcccgccttg gagagctccc ggatgtttcc tttgagagcc
    10681 gagggattgg aatgcctgct cagagcctct tccgcagttg cttggcccga aggggtgatg
    10741 cagccgacga gattggttct gagggcagta cgggcgaact tctgaaggag gaaaagctct
    10801 tccgtggcaa gccccgcgtc cgtcagtacc gccgtttccc atggaccgta gcttcggaat
    10861 ctttctgtaa gctcatccag cacactttcc cactccgctt cgcggtagcg gccgttttca
    10921 aggacgagcg gcttattgag acgatccggc cgctgcaggt agcttttcag caggaaccgc
    10981 ccctgaacgc aggcttgtcc gtggctgccc ttgcggtttc tgtcggggcg cacccggatg
    11041 atttcacccg attcgctcag ttcgaaagcc atgcggcagt ttgcactgca catgggacaa
    11101 atcgatgtct tccattgcgt cggcatctct tttcgataga gattcatgac gatggaggtc
    11161 aacgcttcgc atgcatcgag catctgcccg cgcgcttctc cgtgcgtgat ttcgagcttt
    11221 tccctgagcg ccccgactgg acagacatcc acgcacgccc cgcaaaactg acacccggac
    11281 tcctcgaggg agcggcccaa agcggttccc acttcctggc gtccttcgat ctctagaaag
    11341 accatggcct tcgatcctcg cacctcgtgg cagatgcgca cgcaccggcc gcagcgcacg
    11401 caaaggctca tgtcccgctc gaaataggac ccggcagcct gaaccggggg tctttctccg
    11461 tttggcggcg gatagggaaa atcgatcccg acggcttcga caaggcgctg cagttcacag
    11521 gtcccgttgc gcgggcatcc caggcactcc cgcggatgct cctgcaggac gagccgcagc
    11581 acttcacggc gaaattccgt gaccttgggc gtctgcgtcc tgatcttcat accctgttcg
    11641 accggggtcg agcacgcagc ggggagcccc cggcagccgt cgatctcgac gacgcaaagc
    11701 cggcacgctc cactcggttt gagcgacgga tggtaacaga gccgcgggat atgcaccccg
    11761 gcctggtccg cagcctccat gatggaacgg cccggttcca tctccacttc ctgattattg
    11821 atccacagct tgatggaatc catcttgatc gaattcatct tcacctctga ctgcgatcac
    11881 gctgtgcgca gcatatatgg ggcaaatcac gaaaggatct ttggggagca cgatcagagg
    11941 ctcatgaagc cgaaaccgga ccaagccgct ttcacccgga ttgagccttt gcctctccat
    12001 gagcaccaca agagcattca tgccgcatgt gcccgcatac aacctcacgc gctgccggtt
    12061 cacaacggcc ttgcgcgcct tggggagaac cgaaatcccg acattcggaa tatagccgga
    12121 ccggagggaa ccgggagcag ccagggccac ccccacgctc acatcatcga ttgaaatctt
    12181 atgcagatta ataccgaccc gctgcccgct tgctgcccga tcgactctct cataatgcac
    12241 ttggatgaag cgtgccttgg tttcctttcc cgaaggcaga agttgaacgg gaccgtcctc
    12301 ggaggtgctt cccgcaagga tcgttccgct tgcaacggta tcgaaccggg gaaagctccg
    12361 caccttgtcg atccggagtc gaaacggggt gtcgtcggtc ttgccgctca catccggcgc
    12421 tacagcttcg atggccgcac gaacctcctc ggctcctctt cggtccactg ctgagaaagg
    12481 aatgacgggc ctgtcctccg gaaacgttcc tgcaagcact tccctgattt cgagctctgc
    12541 caactccaag gtttcttcgt ccaccaggtc ggccttgctc agcactacca tgccgctccc
    12601 ggccttcagg aagttcagta cctcgaggtg gtcgagagtc tggggcatga ccccatcatc
    12661 ggccgccacg attagacggg ccagatcgac gctcaacccc cggatggtgt tcttcagcga
    12721 gtcgctgtag ccgggaacat tcaccagcgc aagcatcctt cccgagggaa gcgtgagcgg
    12781 tgcaatgccg ggctcgatgg gacaaccccc gctgcttttc ttctttgaga cggtccgtat
    12841 cgattcccgt aagacacctg accaccctgg tcctcccgtg gtccacatgt cccgcaacgc
    12901 ccgtggtgat aggtttttcc gttggcatga taccctcatt gcgttgcgga tgactctaac
    12961 ggcaccctct ccttgaagag ccggatagcc tgtgcaatgt acttcctcac aagggccggc
    13021 cgatcgatgc ctcgtgattg gcggtcctct tcataagcgg gatgggttga ggcaggtacg
    13081 ggggcctcga gctgagccgc atactcatag gttgccgaat ccatcaccag atcgatgata
    13141 gaccgatctc ccctcggaat tgcctcgatc catcccagtc gtcgcatgca ttcgaaccgc
    13201 gcctgatcgg ttatgaagaa ataggcattc atgagggtcg agaatgtgtc aaaagcctga
    13261 gcttccaagt catggccggt cccaagccgc tgagctccca tgagcaggtc atacaaagcg
    13321 tgcttgctct ccgggtcttc ctcacagaag aacagaacga tttcatccgg aagatctgcc
    13381 cacttcgtgt gttcatcaat gtccgtaacc gaccggaaga ggcgcctcca ttccctgaag
    13441 cccctgtcgg cagccttcct gctcttgtga cattgcaaag agatgatgtc tgccatcgtc
    13501 ccccctctta ccctctttat cagcctgcaa cactgcccga tgctgcctgg atggatcccg
    13561 tttcctgctt tttccggcga aggcccgggc ctagctttct tatgcggtcg gtgaaatcac
    13621 ggcagcactg ggcaaactgc ggtcccatac cggcagagtt atggtacatc tccacccgct
    13681 ccggatccat gccgatttgc gcgagcaact ccttcacgta ggcgactcgc ttcttcgctc
    13741 tgtaattgcc ggaaatgtag tggcagtctc ccagcaagca tcctgaggca aagacgccgt
    13801 cggctccttc ttccagagct ttgaggaaga aaatgacatc gagtcgccca gtgcacggca
    13861 ctttgatgat ctttacgttc gttggatact gaagccgcat ggatccggca aggtccgcag
    13921 ccgaatagga gcaccattga cagcaaaaac cgatgatgac cggctcgaag gcatcttcca
    13981 tggcagacga tctccccctg taagtcgatg gtttttctaa gatatccaaa agcctaccat
    14041 catccggaaa aacctcctgc agcgtggaag agggacgaac ctttatcggt cgccggcatg
    14101 ccacaccatc tctcccggac gggggtatgc tgagttggag ggggcaaggg gaatcgaacc
    14161 acctcacggt tttccagacc gggccaacgg gtttgcagcc cgcggggcgc ccagcaccat
    14221 tgcccccgta attgcctaaa ccaggcaaac gagccggact gaagaagatc tcaaggggac
    14281 tgttcttgga aagaggatgg aacggaagcg atctctcggc ctggccctgt gcctaccaga
    14341 cacgggtagc ttgaatgcag ctacctcagg tgaagccgaa aaatcgatac atgaaaaagc
    14401 tccctgtggg gattgttgaa gattcgggga cgtgaataat cgtaaattga cgatatgtca
    14461 aatcgtaagt ttctataata tccccatgtt gtattgaaaa aagcttgtgc gtctggggca
    14521 tctcttggtt catccggcta acgagtacct ctgggcctca acccgtgggg ttgcctggcg
    14581 ggctgcaagg attcccgggg aaatttctta aaccataagg cagttgcccc tggaaagtac
    14641 cgggatcgtg attaatttca tcctgatgct tcaaggtgaa aacccagtat tatgaagccc
    14701 ttagcttcac aatggcaaga caatggctgt acggatggac ccacattccg tttatgtcta
    14761 tccgaacagt agggaatgag gaggaatgat ccatggcaaa ggcagtggga attgatctgg
    14821 gaaccaccaa ctcggtggtg gccatatggg aagatggaaa ggccacggtg atcccgaact
    14881 cggaagggtc gcgcaccacc cccagcgtgg tggcgtacac ggaagacgga cagcgcctcg
    14941 tagggcaagt tgccaggcgg caagccatac tgaatccgtc ggggaccctt tactcggtga
    15001 aacggtttat gggccgcaaa tggggggagg tcgacgagga atccaagata gtctcctaca
    15061 agctggtgcc gggtccggcc gacgccgttc gttttgaggt gcgcggaagg aagatttctc
    15121 cggaggaggt ttccgcacag atccttcgca agctcgtgga cgatgcgtcg aggcatctgg
    15181 gagagcgggt tacggaagcg gtcattacgg ttcccgccta tttcaacgat gcccagaggc
    15241 aagcgaccaa ggacgctggg gaaatcgccg ggttgaaggt gcttcgcatc atcaacgagc
    15301 ccacttcggc ggccctggct tacgggctcg agaaaaggaa aaacgaaacc atcctggtct
    15361 tcgacttggg cggcggcacc ttcgacgtgt ccattctgga cgtgggggac ggagtctgcg
    15421 aagttcggtc cacttcaggg gacacccacc tgggagggga tgatttcgac aagagggtgg
    15481 tagactggct tgccgaggag ttcaagcggg agacggggct ggacgtgcgg ggagaccgcc
    15541 aggcgctcca gcggttatac gaagcggcgg agaaggcaaa aatcgagctt tcttccacca
    15601 cggagaccca tgtgaacctg ccgttcatca ccgcggatgc ttccggtccc aaacacctgg
    15661 tcatgaaact cacccgggcc aagttcgaag acctggtcca cgacctggtc cagcgatgca
    15721 tgaaaccggt gcggcaggcc ttggcggatg cgcgcctgac cgaaaaggac ctggatgaag
    15781 tcatcctggt gggcgggtcc agccgggttc cggcagtcca ggagttggtg aggcagctca
    15841 caggggggaa gcaacccaac atgagcgtca acccggacga agtggtggcc gtgggggcgg
    15901 cggtgcaggc cgccattctc aagggagaag tggaagatgt ggtcctgttg gatgtcacgc
    15961 ctctctccct gggggtggaa accctgggag gcgtcatgac gaagctgatc gagcgcaaca
    16021 ccacgattcc cgcccggcgg gaggaaatct tcaccacggc ggaagacaac cagacggggg
    16081 tcgagatcgt cgtgctgcag ggggaacggg aaatggcccg ggacaaccgg gttttggggc
    16141 gatttcgcct ggaaggaatc cgcccggctc cgcgaggagt gccccagatt cgggtggttc
    16201 ttgacatcga tgccaacgga attctccacg tgaccgcccg ggatcaggag acggggttgg
    16261 agcagaaggt taccatttcg gaatccacca atcttgccaa gaacgaggtg gaacggatgg
    16321 ttcgggaagc ccggcaatac gctgccgaag acaaggagcg ccggttgacc gtcgaggcta
    16381 agaaccaagc ggacagcatg gcctaccagc tggatcggat gctgtccgaa ttgggggaca
    16441 gggttcccgt ccatgagaga gctcggggag agcaattgat ccgggaggtg cgtcgtgcgg
    16501 tccgcgagga gggcaccgat cgcggacagt atcagcaact agcgggggac ctgcagcagg
    16561 ccctccaaat gctcactgca gcggcacatc aagaggcttc ctccgcctct gcagggggcg
    16621 ggcaggcccg gtacggatcc cgagggcgag ggggtgacga cgtcattgac gccgagttta
    16681 gcgagaggta aacgaagcaa tcaccggtga gatggtttcc atgcccgata atccaatagg
    16741 gaagaggttt tgacgagagc ctatggccat caagttcaag gactattaca aaacattggg
    16801 ggtttcgcgc acggcttctc aggaggaaat ccagcgggcg tatcgaaagc tcgctcgaaa
    16861 gtaccatccc gacgtgaaca aagcctccaa cgcggaggag aagttcaaag agatcaatga
    16921 ggcctacgag gtcttgaaag acccggacaa gaggtcgaag tacgatcagc tgggccccga
    16981 ttggcaggga ggacaggatt ttcggcctcc tcccgggtgg gatttccagt atgatttcgg
    17041 ccatggccgg ggaccggaac aagggggatt ccagtggtcc ggcgggacgg gtttcagcga
    17101 tttctttgaa gccctcttcg ggggacaggg ctttcggggt gccggaggtc ggggggcaag
    17161 gaagggaccc gtttggagtc aagcggggtc cgaccaggaa gccaccattc ggatctccct
    17221 cgaggacgct tttcacgggg ccaccaaatc catcgccctg caggtgcaaa ccgtgacgcc
    17281 cgaagggcag ttgtccatcc aatcgaagaa ttacgacgtg aagattccgc cggggatttt
    17341 gcccggccag aagatccgcc tgggaggcca ggggggcgaa gggattggag ggggaccgcg
    17401 gggcgacctc tatctcaagg tcgaaatcga gccgcacccg gtctaccgtc tcgagggcag
    17461 aaatctattc atggatttgc cggtcgctcc gtgggaggcc gtactgggtg tggaagcccg
    17521 gctgcccacc ttatcgggcg cggtcacggt aaaggtcccc cccggtaccc agaacggccg
    17581 gaagctccgg ctgcgggcca gaggaatgcc caacagcaga ggggctcccg gagacctcta
    17641 cgccgtgatc cacatcatgg ttcccacgaa gccttcggcg cgggaaagag agcttttcga
    17701 ggagctccga aaaacgtccc ggttcaaccc ccggtctgga gaggaaggat aggcagccat
    17761 ggcggatcgg aaatatttca tggttcaggt ggggcggtat tcgaggagct tcccgttcct
    17821 gacggccgac gaagtggcgt ttcgttgtgg cgtgcatccg gagctggtgg agcgattcct
    17881 tcaactgggc ctcatcgaaa tcgcggaaaa ggattggaac ggcgagcccc tgttccccat
    17941 ggacgtcgtt cccatggtgc ggaagatcct caggctccgt aacgagctgg gggtcaacta
    18001 cgcaggaatt ggagtgattt tggagctgat gacccgaatc gaatccctgg aagccaggat
    18061 tcgggagctg gaagacgagc tctttggagg tgtttgaggg agtaccctcg atggggggtg
    18121 gcgtccgcag gaagagcgcc ctcggcacgg tccgaagtaa acatatggaa gatggaggat
    18181 ctcataatat ggacctaagc aaattcacca tcaagtctca ggaagccgtt caggcggcac
    18241 aagccaaagc catacaatac ggccacatgg aagtggacgg agaacatttg ctgctagttc
    18301 ttctcgatca gcccggaggg ctcgcttccc ggctgctcca gcggatggaa attcccgcgg
    18361 agagggttcg ggagcgactg gaggaggaac tggaacgaaa gccccgggtg agcggcccgg
    18421 ggatcgagcc cggcaaggtg tacatctccc agagggtgag caaactcctg gtccaggccc
    18481 agaacgaggc caagagactc aaagacgaat acgtctccgt ggaacatctc ctgctggcct
    18541 ttgtggacga aggagccgca acgcccgcag ggcgggtgtt gaaggaattc cagctcacca
    18601 aggatctgct gttgaagacc cttcacgagg tgcggggacg tcagcgggtg accagcgccg
    18661 accccgagac cacctacgag gcactccaaa agtatgggag ggacctggtg caggaggccc
    18721 gttccaacaa gctcgacccc gtgatcggcc gggatggcga aatccggcgc atcgtgagga
    18781 ttctcagcag aaagaccaag aacaatcccg ttctcatcgg agaccccggg gtcggaaaaa
    18841 ccgccatcgt cgaagggctg gctcaccgga tcgtccgcgg ggatgtcccc gagggactga
    18901 aagacaaaac catctacgcc ctggacatgg gtgccctggt tgcgggagcc aagtaccggg
    18961 gggagttcga agaacgtctc aaagcggtgc tccaggagat caaggagtcc gaaggacgca
    19021 tcattctttt cattgatgaa ctgcacacca tcgtgggggc gggcaaggcc gaggggtcca
    19081 tggacgcggg caacatgctc aagcccttgt tggctcgggg agatctgcac tgcattgggg
    19141 cgacgaccct ggatgagtac cgcaagtaca tcgaaaagga tgcggccctc gagcgccgct
    19201 ttcagcccat cctcgtggat cagcctacgg tggaagacac catctccatt ctgcggggac
    19261 tcaaggagcg gttcgaggtt catcacggcg tgaagatcca ggacagcgcg ctggtggctt
    19321 ccgccgtgct gtccaaccgt tacatctcgg atcggtttct gcccgacaag gccatcgacc
    19381 tggtggacga ggcgtgcgcc atgatccgaa cggaaatcga ttcccgcccc gaggacctcg
    19441 atgaagtcag ccgacgggtc atgcagttgg agatcgaaga ggtggcctta aagaaagaac
    19501 gggatcgggc gagtcatgaa cggctggaaa ccctccggaa ggagctggcg gagttgagaa
    19561 gccgggcgga cgcaatgaat gcccagtggg aatcggaaaa gcaggccatc aagaaggtcc
    19621 aggccctccg ggaagagatc gagaaggtcc ggcgggaaat cgaggtcgcc cagcgcaatt
    19681 tcgaccttca ccgcgcggcg gagctcaccc acgggcggtt gccggacctg gaacggcgtc
    19741 tgacggcgga agaggaccgg ttgagttccg agcatggggg gaatcggctc cttcgtgagg
    19801 aggttaccga agaagaaatc gccgaaatcg tctcccgatg gacggggatt cccgtggcca
    19861 agctggtgga aggggaaagg gagaagcttc ttcggttgga cgaggtgctt cacaggcgag
    19921 tggtcggcca ggacgaagcg gtcaggctgg tggcggatgc ggtgattcgc gcccgttcgg
    19981 gcatcaagga tccccggcgt cccatcggct ccttcatctt tctaggtccc accggggtcg
    20041 ggaagaccga gttggcgaag accttggcgg aagccctctt tgacagcgag gacaacatcg
    20101 ttcgcatcga catgagcgaa tacatggaaa aacacacggt gtcccggctc atcggggccc
    20161 ctcccggcta tgtcggctac gaggaaggcg gtcagctcac cgaagcggtg agaagaaagc
    20221 ccttcagcgt cattctcttc gatgaaatcg aaaaggccca tccggatgtc ttcaacgttc
    20281 tgctccagat cttggacgac gggcgactga cggacgccca gggacgcacg gtggacttca
    20341 agaacaccgt catcatcatg accagcaaca ttgggtccac gcacctcatc gagggagtca
    20401 ccgagggtgg ggagatccgg gagtccgcca ggaagcaagt gatggccgaa cttcggaggg
    20461 cctttcgccc ggaatttcta aaccgggtgg acgacatcgt gctcttcaag gcccttaccc
    20521 tccaagagat cgaaaagatc atcgatcttc tgacccagga ccttgtcaag aggctcaaag
    20581 atcgccgaat cgccctcgac ctttccgacg aggcccgtca attcattgcc cgggctgggt
    20641 atgatcccgt atacggagca cggcccctga aacggtacct gcaacatcgc ctggaaacca
    20701 ccatcggccg tgcgctcctg tcaggagaca ttcccgacgg gagcacgatt caggtcgggg
    20761 tccgggacgg cgagctgcat gtgacccata catcggccca gtgacggccg ttgccggaac
    20821 gattcagggt ggaaaccggg cgaaaccgcg tgccgcccag ctctgaggcc gtaggattct
    20881 cccggtttcc tttccgggtc tccccatgat cgaggcaagg caaacgatct caagtagttg
    20941 tcgactcccg agggctccct tgggagaaga cgggaaaagg ggagatgaat tcatgagaaa
    21001 agcggaggaa aaagaagttt gatctaggcg aagaaggagg gtcgacttcc gaagtcccgt
    21061 aagttccggc tttaacgtcg gaaattcttt tgctagttct ctcttgccga tcaataagtt
    21121 aggcccccgg aaagtccaac atccattcat tccgggaaaa ttttcccccg aaacctcccc
    21181 ccgtgatctc ctgaagaagt cgtgagatca cctttgacct attcccgggt ccccggtatg
    21241 aagggaccac tcttgggggg agacgtggca aaatgctgtt gcaccgtttc cggattcgcc
    21301 cgtaccaaca atcagacacc gtgtcttcca cattcgagaa tcgacgtctt ggcagggagg
    21361 ccccgcaggt ttgctcccag gaccgaggcg ggagaggttc gtttcattcc attgaggtga
    21421 cccaggggac ggcttcggcc tggttcagga gtgggagggg gcagtatgga ccagcagtgg
    21481 cggcagatta aaggcgagct cgaaaaggtc ctctccaggg gacagtatga cctttgggtg
    21541 accaccatcg agtttttggg ggtgcaggac gggcgccttt gcctggggtg ccggagccga
    21601 ttccatatcg agtggctccg ggggaaactg gaaaataagc tcctcgatac cgcccgggct
    21661 ttctttcctc aggtgaggag ggtcgagtac gaggtggtaa aggacgtgga ggacccccct
    21721 gcggaagccc gggaggattg gccccggcag ctctcctttc atgaagtggt ccggcgcccg
    21781 gaacccgtat acaaccgtcg attcaccttc gatcaatttg tcgtgggagg ttgcaaccag
    21841 tttgcctacg ccgcctgcct gggcatggcc agcggccagt gtctctacaa tcagtcggtc
    21901 tacctgctct cggaaaccgg cctgggcaag agccaccttt cccatgccgt gggcaactac
    21961 ctgctgagct ccaaaccgga aacccgggtc cactacgtga cggccgagca gtttgccaac
    22021 gagatgatca gtgccttgag aaacgggagc atggaagtct tcaagagcaa gtttcgggcg
    22081 gactgtgatg tttttcttct ggaaaaggtc gagtttctca gcggaaagga gaaggtccag
    22141 gcggagctgg tctataccct ggacgagttg atgaaccgtg gaaaaaggat tctatgcacg
    22201 ggaagctccc accccaaaca gatcccgaag ctcaactccg atttgcagtc gcggctgagc
    22261 ggcatcctgg tggctcccat cgagccgccc gatttcgaaa caagggtgga aatcatccgg
    22321 agcaaggcca agggggagaa cctggtgctt ccccgggggg ttgccgaata cttggcggag
    22381 cgggtttccg gggacgtgcg caggatcgag agttgcctgg tggggttgat cgccaagacc
    22441 aacatcctgg gggtcccgct gacccttcag cttgccgagg aagtcaccca gacgatgctg
    22501 gagaacctgc ccaagatcga tatgcaaagg attcaggaag tcgtgtgctc ggtctttcag
    22561 attaccctgg aagatctgaa gtctccttcc cgaaggaagg aagtcgccat ggcacgcaag
    22621 atgggcatgt acctagcaag gatgtacacc tcggagtccc tggtgagtat cgctcggtct
    22681 ttccagaggt cccacagcgc agtggtccac gcggtgaaag acctcacccg ggaaatggaa
    22741 gacagcggca gcaaggttcg tcggcaggtg gaatatgtaa gccgacgcct ggaaaccacc
    22801 tgcctgacct catgaggggg gaaggctttt ccgtcccccc gatccccgtg gagaaggact
    22861 cggatgtttc ccgggaaggg tctacttggc gcgattgatc ttttctcgaa ggaccccgga
    22921 acacttgaag gtgacgacct tccgggggga gagcatgatg ggttcgccgg tttgagggtt
    22981 ccggccgcgg cgctggttct tctccttgat gctgaatttg ccgaagccgc tgatgagcac
    23041 gtcgtccccg tcttccaggc tctgcttgac gatttcgaaa agcgtctcga tgatgtgagc
    23101 ggattcggtc ttggtgaaaa tattcttggc gaacagattc tccacgatat gggccttcgt
    23161 tagggtcata cgcttttcct ttccacgcat cggatcttgc ataccgaacc ggccggccga
    23221 atcccttttc cgaattcctc aattttccta ggctagtaaa cgataattcc tcaataatca
    23281 acacaaaaat ggggtccctc ttgcgaggaa cccctccctt ggcttccttt ggtggcggtg
    23341 cagggcttcg aaccccggac actgcggata tgagccgcat gctctgacca actgagctac
    23401 accgccgtcg agcgtgaaga aaggtaaagg aaaaccgcag ggtcgtcaac tctaaaatgg
    23461 aggtccaacg ccccgagacg gaagggcgtc gggattagaa ttgacaaaaa cgaccctttc
    23521 ggatagatgt ggttaactca ttgatgctaa gggctttttt catttttctc ggagaagttc
    23581 ttagcctccc cggctttcgg gtcttcccgg ttggatccgg tggggctgtt ccttcacaaa
    23641 cgctgcgggc cctcggggcc gtgtggcgag gagaggctca tttgaaccca ggcgtgtacg
    23701 aagtcaaagt aatcagtgat ttttcagcag cacatcttct acggaatttt cgcggcaaat
    23761 gcgaaaacct ccacggtcac aactggaaaa tagaggtggt tttgcggggc tcgaagctga
    23821 acgaggccgg gctccttatg gacttcggag aaatcaaaca cgccacccgg gaccttctcg
    23881 gagagatcga tcacaagtac ttgaacgatc ttccttcttt caaggaccag aacccttcat
    23941 cggaaaacat tgcccgttac ctgtttgaac agctttcggt tcggttgaac acggaaagtc
    24001 gatacgtttt cagcgtatcg gcctgggaat ctgcggatgc ttgtgccacg ttcgtgcgcg
    24061 agtgagtggg actaaagggc tgaggataat aggagcagga agatggcgct gaggaaacgg
    24121 atccatccag tggtgaaaca aagggatttg caccggagac ccatggtctt cgtgctgggg
    24181 gccttactga cttgcctggt ggtgatggga gtctcgcagc atgtgtgggc tcttcaacgg
    24241 ccgggcggcg ctgccgaaag cccgggcggc ggtgccggga ctgcggctgc gcccgttgcg
    24301 accctccctg cgccggagac cccggatgcg catgcttcgg ccgcggttca gaagctggcc
    24361 gtgagtccgc cggctcatgg agagaaggcc gccgtgcctt ctcacggagt tccctccgtt
    24421 ccctccgtcg gagcggaagc ccatgctccg gcccccggcg ccgccgccca cggagaagca
    24481 gcccatggag caggtcacgg accggcgctg cccgagatct ctcccattcc cggcgtgacc
    24541 ttcgtggaga ccatgatcca gctcatggat cacgagctca aagggcgcaa gctgggatgg
    24601 cgccccaacg acatcttttt cggcaggttc accgacaatg tcaacaactt ccaactgggg
    24661 gttctcgagg ctttgaggtt taccacgctg cgcctcaagg acagcctcac ccggatggga
    24721 gacgccgaca cctatgatcc cgacctggaa gcggccctga acctgctcat gaacaaggcc
    24781 actctctttt ggtttccatc cgccgagagt tcctacgggg atgcggtgga tcatctcagg
    24841 gccttcgtga ccaagctgaa aaccggcaag cgctccttct actaccggaa agacaacctc
    24901 gtctccctcc tcgcggctta tcgggacctg ctgggcaacg tcaacaaaaa cctgatcaag
    24961 ggagatgtga gctggtttaa gacggacgac tatttctatt acgccaaggg tgtggctcac
    25021 gtctattacg agatcctgcg agttgtgcgg gtgggatttc aaagccagct ggcttccacc
    25081 atgtacgctc tcgacatgat ggatgaagtg ctccatgagc tccatattgc agaggggttg
    25141 gacccgtgga tcatccttga ttcgaagctc gacggcttcc ttgccaacca ccgggccaac
    25201 ctcaacgccc cgttgagcga agtggtgcac gttctggtga tcttgagcca gctctaagga
    25261 ccccgggagg tttctttccc agagtcctga tttgcgaggc gggccttcca acctgcctgg
    25321 tggccgtcca ggtccgacag gaggggaggc ccgctgccgg ccatgtcccc tttacaaacc
    25381 cgtcccggta acccgaggcc caaactctcc ccacctccag gacccgttac cggttccttc
    25441 ggcaagtgag ccggttgtcg ggcgcttccc ggcgacggtc agcccctctt tcgttcgccc
    25501 tgatcccggg catcctcgaa cttctttttc tgacgatgga aataatccgg gtttttcggg
    25561 tgaagttgca gggccttgtc gatggtttcc agagcactct ggaaatctcc gttcacaaag
    25621 tacgcctcgg caagggtatc caggatgtgc ggttcgtgct ccagttgcgc cgcacgagat
    25681 gcgagccgga gagatgccgt gggatgaaag tacggaggag gggcggtcgc atagagccag
    25741 gccagattgt ttaggaccgt ggcgttttcc gggagaagcg ccagaaccct ccgaaggact
    25801 tcttccgctt tcccgtattc ccccagttcc aagagggctc cgccgtaaga agcggcgagc
    25861 atggggttgt gcggagatct cttgatctcc tgttcgaagc ggtgtgtgag cacctcgagc
    25921 ccccagcgcc ggaccgtttt cgaatccttc caatagaggc ccagggcgga aagaagaagg
    25981 atagccgcca aataaacggc tcccgcgcga tacagcttgc gatcatgagc ccggatgagg
    26041 ccgggattac gataggaatc caccagacat cgaatcctct ccccgatacc gtagtggtgc
    26101 cagttgggca gatcccgaat ctggccgctc catacggcga tcttttccag ggaggaaatc
    26161 agaaagaagg gatggccgat gagccggagg gcatagaggt ctgcctgtcg ctcgctgttt
    26221 cggagaaagt acccgaagac aaaccgtatg tagacgacca gcaggaaaag gatgggtagg
    26281 ctgtagagga gggaaaaaag gttgtgatcg atggccgccg gcgaccccgt cagatcgaag
    26341 acgaggggat ggcgcaacac cgccagcaga atgatctgat tgaaggagaa ggcaaaaaag
    26401 gcatagagga gaaaaaagac caggtagaca atcatgtgat aacgacgcac atgccccatt
    26461 tcgtgggcaa ccaccgactt gagctcttcc gcattcaaca gggacagcag tccctgagta
    26521 accaggatgt agcggcggcc cggaaggatg ccgacgattc ccgccgtaag catttccccg
    26581 ccaaaaagag gccacagaag aaggtctccc acccggaact tccgttgccg acagaattcc
    26641 tcgagctccg cccgcacggg agattccgga atgggcttgc atccccacag ccgcacgacc
    26701 caggggggag ccagcaacag gaagagaccc atgaccaagg aaagcagcag aaactgtccc
    26761 ggatcggtct gcaagaaacc cggcccgccg agaatctgta caaggtcgga gacggtcgaa
    26821 atgaggatcc atggaatcag aatggccgac ccaaaggcca ggtgccctcg caaaaaggct
    26881 gggagcccga tcctggattg atgaatccgg cggtaaaagg gatggctcaa ggtccacagg
    26941 acgaggaggt gcaggaggta gacggccagg cccgtgagcc cggcaagggt atacgactga
    27001 tcgaaccacg gcatccgttg caggtaaacc ttgatgtcca ggacataggt gtagaggacg
    27061 agagccgcca gggcgagaac ggacaactgg gtttggatcc ggtgatatgc cgccgtgaga
    27121 gccgaggaag gggagtggtc cctgaaggtg cgggcaaggc gccgaaagag aagaatgctg
    27181 atgaagacga aggttccgaa cacccccagg caaagcagaa aggtctcgag gggagggtga
    27241 agaggccggg accccggttg ctgagcggag taaagaagga tcgccgccac gaagtaaagg
    27301 agttgattgt acatggggtc cctgggatgc aggtatggtc gggacggatt gcaccgaacc
    27361 ggaccgtgga aacaacccgg tcccgaagga ctccttcgga accggggcga aaccgtccac
    27421 ggactataac cgaattcggg ggagggggcc agactcatcg ggccggcccc caccgatccc
    27481 catgcggaat gaatgggaaa gatccccggc agatcccccg ttaaagcttt ccttgacttg
    27541 atttttgccc tgtgcaaccc ttgtgctcca gtcccgagct ctcattcgac cggcaaaaca
    27601 gcagacccat ggagaaaagt gtgcggcgca tgatactaaa gatcctggcc ggttttgcgg
    27661 cagtgtatct actggtcgcc ttggtcgttt tcctgttcca ggcccgtctg gtctactttc
    27721 cggaacgcga cacgatttcc actcctcgga gcgcggggct ggcatacgag gaaatcaccc
    27781 tccttaccga agacaatctt cgactccagg cgtggttcgt gcccgcacag aattcccggg
    27841 ccgtcctgct cttttgccat gggaacgccg ggaatatttc ccatcggatc gactccatcg
    27901 cactttttgc ccgcctgggc ctgagtgtgc tgatctttga ctatcgggga tacggtcaaa
    27961 gtgagggtac tccctcggag aaaggcacct atctcgatgc cggggccgcg tggcgatacc
    28021 tggtccaggt cagagggacg gcacccgccg acatcatcct tttcggccgc tccatgggcg
    28081 ctgccgtggc ttcctggctt gcagttcggg aaaatcccgg cgggctgatc ctcgagtcgg
    28141 ccttcacctc cattcccgat atggcggccc ggctctatcc tttcctgccg gtgcgaattc
    28201 tgtcccgcat ccagtacaac accctggaga acgtccagcg gaaacgatgc cctctgctgg
    28261 tggtgcacag cctcgatgac gagatcgtgc cgttcagcca tggacggcgg atcttctcct
    28321 cggcccagga gcccaagaag ttcctggagc tcaagggaga ccacaactcg ggctttttga
    28381 tctccggttc cctctacctg gaaggtctga gcaattttgt ggccgcgtac ctcaaaaaat
    28441 gacgggactg agggggatgc gggagattgg actccctcga ggctctcctt ggggatacaa
    28501 tgcaggcaag gaccaacagc gaaggttttt gccaacccga caacaggggg aacccgatga
    28561 aagattaccc cgaaaggttc gcaaggattt ccggccgtct catgggggcg ttcctggtct
    28621 tggtgctcac cgccgcctgt cagaccatct ctttggacta ccgctcgggg agagtcctcg
    28681 aacaggatcg gatcgctctt ccggacaacg ccgaacaaca gggagtgtgg aaaaccaggg
    28741 aagccgtggt ggaatatcga tttgcccgga gagggaacga gctgaaaatc tcgggagtca
    28801 tgaagttcgc cgaacccatc accaacaact tctcgggaat cgagcattcc cacctggatg
    28861 cgatctttgt gaatccgcag gggaaggtct tggagattag agggttgatg agcagtgcct
    28921 ttgcccaccc ggaatctccc caaaagttcg agaaccggct gagcctgcct ccgggtaccg
    28981 aatccctcgc cttcagctat caaggaagag cgttctccag cggactcgac aacaacgact
    29041 tgtactcctt ctggtattac ccaatccacc ccaagtgaag cccaaacggc ggggaggatc
    29101 gtcgaggaag cgcagcggac cgggaggtgc ggaccgttgc gccccaagtt tagggggatc
    29161 ggcgtcttta ccggcaatcg cctttgggga ggaggacggc taggggagga cagggacggg
    29221 aaaagcagtg attccatcct ccgtgatcgg tgcatgcctt gccgggtttg ccgggacgat
    29281 cgcacaggtc ctggttttgc gggaattgct ggtcctcttt tgcggaaacg aactctcatc
    29341 gggcctgatt ttcttctgct ggctcctgtg gaccgctggg ggcagtgccc ttgccgcgtt
    29401 ccgatgcccg aagaagtggg acccctcctc gatgctggtg gtcaccctgg cgctcatggc
    29461 ggtggtcttc cccctcaccc tgctggggat tcgcggggcg aggtccctgg caggaattcc
    29521 cctgggcgat gtggttccgc ccgggtggat gctcctgatt tccatgggat ccaccggtct
    29581 tttctgcctg actctggggg cgcttttctc gctgacctgg gcggtgcacg tccggatgtc
    29641 cgggagtcga gagccgaggc ccattcttgt ctacggtttc gaggccgtcg gagcggccct
    29701 ggcgggcctg cttttctact actgccttct cccttacttc tccgcccttt gcatcagttg
    29761 gatggtctcc tccgcaatcc tgtgcgccgc atgggcgcta tccctcaggc tcgaagaggt
    29821 cgcctcttcc cgaacccccc tcttcaggtt cggtcttccg ggtttggtcg cattgaccct
    29881 ggcagcggga tttgtcctgg aggacctgga ccggttgagc cgtcgttggc agtggggcga
    29941 tgccctcgtg gctgtggaag acaccccgta tcaaaacctt gccttggtga aaaacgaaga
    30001 ccagctcacc ctctttggga acggagtgtg gcttttttca gtccccgacc cggagacgga
    30061 ggaatatgcc gtgcacctgc cgttgcttca gcatccaaac cctcgaaggg tgttgatcat
    30121 cgggggaggg acccggctgg tcggggaggt cttgaaacac ccgggtatcg aggtgctgga
    30181 ctgcgtagag ccggaccccg gggtgatccg actgatccgg aaggcattgg gggactcccc
    30241 cccgcaaccg ttccaggacc cgagggtccg cctgcattcc gtggatgcca aggccttcct
    30301 aacccatgcg ccgggagcct acgaggtcat ccttatgaac gtgggcgatc ccgccaatgc
    30361 ttccatgaac cgcttttaca cggtggagtt cttcgagcgg atcggaagcc tgctgaagcc
    30421 ggaaggagtg ttttcctttt ctctctcggc ggctcccgag atcctgggac ctgcccaggc
    30481 acgcgccatg gcatcggcca acagcaccct tcaagccgtg ttcagccgag tgaccctggt
    30541 cttgggccac ggggcacggt tcctggcgag caacccccaa acgttcttcc tgctcgaacc
    30601 cgaacctctc gcggaaatca tccggcaaag agggctcacg ctccagtatt ttcaagccgg
    30661 tttgcttgcg gactacctga gccctttgcg ccgggcctat gcccggtccg tactcgagaa
    30721 aagcggaccc gcccaggaga atcgcgattt ctcccccacc tgctatttcg atcatctcat
    30781 cgtatgggct ctgcaacttc atccgcgatt gggagacacc ttcgtggccc tgtctcgaga
    30841 aagaaacctt gtcctctggg gtctctgcgc cctggggttc ctcgtcttcc cggtcatgta
    30901 ccgggccggg cgttcccgta ggacgcttcc ctttctgggt gtcgtggccc tggtgggggc
    30961 cgcccagatg aatctccaaa tcattctgct gctggcgttt caaatcctgg ccggtttcct
    31021 gtacaaggat ttgtgtctca tcattgcctt cttcatggcg ggaacgggaa tgggtgcggc
    31081 cgcgtccctt tatctccccg gggggctgag aagtcgcccc cggtgggtgt ttgggctgca
    31141 gggggcggtc ggactgtatt cgttggggat ctggggcgta ttgtggcttc tccatgcccg
    31201 ccaactgcat gtccaatggg tcccgccgct ttctgccctc ttctgtctcc tgtccgggat
    31261 ttcgggagtg ctgggcggtc tgcatttttc tctgagcgta gaggccgtat ccttgagcaa
    31321 ggcaccgtcg gagaaaaccg gcgggggtct ctacggggtg gacctgctgg gaggcagcgt
    31381 gggggcgctg gtggggtcct tgctgctcct gcctcttttc gggattcccg ccaccttggt
    31441 cctgcttgcc gtcgcctgcc tgggcggctt gatcttgctg ctgcccgccc tgcaaagggt
    31501 cgacggcccc cgcccggaat gaagggcttt tgggggaatt cctaggttct tcttgcttcc
    31561 ctctggttcc agatccccgg aaggagggtc ccgcagttcc gacatttgcc gttgtcgagt
    31621 cgggtaaggc ggacccggta gccgacccgc tcgatgacta cggtcctgca ctggggacac
    31681 tgggtgtgtt ctgcggaatg accggggatg ttgccgatgt agacaaagtg aagtccttcc
    31741 tccccggcga tcttccacgc ctgttccagg gttgccaccg gcgtgggggg aaggctcttc
    31801 agctggtaga gcggatagaa tcggctgaaa tgcacgggga cgtccggtcc cagttccgct
    31861 cgaatccacc ggcacatggc ccggacctgt tcgggagagt cgtttttgcc gggcaccacc
    31921 aagttgacga tttccgtgtg aactttcaac tccctgagcc gcttgagcgt agccagcaca
    31981 ggctccaggc ttcctccggt catctcgcgg tagtattctt cggaaaaacc tttgaggtcg
    32041 atgcaagcgg catccaggac ccggcaaagg tctctcaggg gggcctcatg gataaaaccg
    32101 ttggaatgca tgaccttgag gatgtccgcc tcttggcaga ttcgcccgat ttcgagcatg
    32161 tactcgagga aaatggtggg ttccacgtag gtggaagcga tggagcggca ctggctcgtc
    32221 cgagccagtg ccaccacctg ttccggggtc aagtcgtaat tgaaggtgtc gtcgggctgc
    32281 gcctgggata tctcccagtt ctggcagaat ttgcagttca gattgcaccc cgccgtagca
    32341 atggaaaacg aggtcgatgc ggggagcaca tggtagaagg gcttcttctc gatggggtcc
    32401 acccggacgg cgcaaggatt cccgtagacc aaggaataat accgaccgcc ccggttctcc
    32461 cggacccggc agtgccctcg ctccccgtct tccacttcgc aggagcgcgg gcacagatcg
    32521 cagcgaattc ggttcttgga caggggggtg aaatagggag agagttttct cctgatgagt
    32581 cccttttcca tggattgggc cgccgcggac ggtggggtcc gaaaaggact gcccaggttc
    32641 agaccggccc agaaggctgc gcagcccccg cagctcttga ggaaatggcg tctggaaatc
    32701 atcggaatct tccttctccg tccgggcatt gctttcccag cccggtggct ttcgcccgtc
    32761 tccggaaccc aagccgggga acgggtcaga aaacgtcggc cgaaaacaca tagatctcgg
    32821 ttcggggatc cttccacgca tcccggggca gtcgcgcctt gttgcaggtt tgctcgagga
    32881 aggtctgccg gtcccacccg tgttccaccg ccacctgggg cagcaaaagt cccgcagagc
    32941 cgtttctccg gacgtagaga ccgtgggtgc ctacctggat ctcttcgggg ctcgagacct
    33001 tcctgaacgg agtgagggcg gatatttcga tctcgatttc cttcaattca ctcgacgtca
    33061 cgggggggaa ccgggggtcc cgggtggcgg ccgccaccgc catttcggcc accgtctccg
    33121 ccagcggttg gtcggagatg atccgcccga tacagcctcg aagctgtccg tgtttgttga
    33181 gggtgacgaa ggcgccccgg ggttcggcca gctttggaga cgtggaaggg agatgcggag
    33241 cctttttccc tgcacagtga gcctcgatcc gctccctggc gatcttgcgc agttgggcct
    33301 tgtcttcgga ggaaagtccc aggtccaccc caactcgcgg ggagttcgac ggggaaggct
    33361 ccggcccggc cttcacattc gaatcggccc aaatggcagc ggccatgtat ccgaccacgc
    33421 ggttgagatc tcccgccaca tccccggaat tggcatagtg gaggacttgg gcgcgattgc
    33481 cccccatggc cctggcggca atcatggcgg tcaccatggg gcctccgccg catgcctcgc
    33541 aaaggttccg ggagagcgac cggctgagtc cttcgggatc cagtgccgcc actctctcct
    33601 gcacgacctt gtccaaagac ctggcctgat cataggggtg aaaatgggaa agatccgagc
    33661 tggccacaag gaccgcggga cgcttctcga tgcatcggac cagggcatcg gccaaccatc
    33721 ggcacgtctc caggtcctgc tcccccataa ccagggccac gatacgacag tccggggccg
    33781 ccacctggag gaaaggaagc tgaacttcca cggagtgttc cttctcgaat gcatctgccc
    33841 ggagttggat tttgggagct tcttttccca ggcagtcggt catggcgcgg tccacggaaa
    33901 gaatgcccag gggggttcga aaccccgatc cgggatacgc ggcgaccccg gaaaatctca
    33961 tatggtggct gggggcgatg aggatcaccg tcgaaggctt ctccgcccgg aggagccggt
    34021 aggaataacc agccgttgcc ccggaaaaca ggtagcccgc atggggggat atcaggccca
    34081 ggagtctccc cgaggagtct cctgcgggaa ccctctcgag gtagccctcc accaatcggc
    34141 gcagctcgga gggggaatcc gggtaccagg aaccggcaaa aaccggctcg cggattttcc
    34201 gggcatcgga gtcggaatcc tttgcccagg gatcccccgg acccaggagg cacgaccaca
    34261 gggcggtgag aaggactgca ttcccgagaa agcggacaca tccgggtcgt ctggaaattt
    34321 gcatcgtctc tcccctccat cttccgtcag agatttgcgg gtggtgggac ttcccgcctt
    34381 gcggcacccc cggcgccgcc cgcgggaaag gatcggttca agccggtgaa aactatgcgg
    34441 tttcagcgtg aggacaatgg gttcgaggtc attataggca accgctctcg gcgcagtcaa
    34501 gtccggcggg aaaatgccgg gtagcggcac caacggcccg gagaaaccgg cgaactgccg
    34561 ggggagcagc ggaaaggggg aactcggctg ggagagttcc gggcagaatc gaggttggag
    34621 ggtcaacgga cgggagagaa agctccccac ggagtggcca gacgctccat cctcttccga
    34681 attcgggaga gggtctccgc atccggttca aaaggaagaa cgggcgttgc agggtccggt
    34741 agagcccggt ccggggaaaa gggggtgagc tccagtttga gattgtaggc cttgaccatg
    34801 aggggctcga gattggctgc atccatgagg ctgtatgcca ggagggtttc caaggccttt
    34861 gggtcctttc tgcggcggta ttcatcccag agcagaacgg caaaagatcc gtccactcct
    34921 tcgagtttgc cccgatccag acccaattgg cgttcgcagc ctttgagccc tccgcggtac
    34981 cccaggctct ttaagaggta tcgaaggtct atatggacct ggtccatggg gattcccagg
    35041 gtggagcgga tgaacggcag gtcaaagcac ttgccgttgt aagtgacaat catccggtat
    35101 tttcggacgt catcccggaa cttccccagg ttgtggccga acaggtaagg ggtaaggttt
    35161 gtgccgtcat agagggcgat ggtggtgatg tggtccgagg gggaccccag ccccgtggtc
    35221 tcgatatcca ggtaggcggc ggcatcccgg aactcggcga acaagcgcca catctcgaag
    35281 gagggcaagt ggcgtgcgaa gaagcggata tccttttctt ggagacttcg caaggaagct
    35341 tccatgtgct gcctgactcc caccgcacga gggccccagg ccgaaggcgg ctgctcgagc
    35401 aggtcctccc aggaaagaat ccccgatgcc cagagctttc gctccgtgat aatgccgatt
    35461 ccggggacat ggcagaaggt gtttctcagt atgccgggca tggcgcgggc cggattcgcc
    35521 acggggatcg ggatctccgg tgtgaggttc tggttgtgag ggaactccct attccgtcca
    35581 gaggggagga cgggctcggg ggacccttcc gccatgggat cgttccccat gggcccactt
    35641 gatgaaagaa gagctactgc tgggattcct ctgtttttgc cgccccggat ccgctttccg
    35701 aattcttttg catcatcggg cagcaggaag gcttttcctt cttgcccggg tgcatggaat
    35761 gcatctctcc agggtccttg cccatcatca tgcactcctt ggatccatgg gccaggcgca
    35821 ggattctctc ccgcatatcc tgtcgctggg ccgccaattc gttgatcacg gccatcatgg
    35881 cggaaatcct ctgttctcct ttcgctttgt tcatggcggc cagtttcgcc tggagacgag
    35941 catccgcggc cttccattct tccacgagct tgccgtgatc cactttcatg gtcttatgga
    36001 cggcagcaga ctctttctcc cctgcgggag gaactgcatg gtgctcgtgg gacatggcgg
    36061 gagactcctg gggagcggtc ttttccgcgg gagccttatc ctgcgcacaa gtccagaccg
    36121 gcaacaaaaa aagagtcaac agcacgagaa tcaaccaacc acttggcttc atgacacgat
    36181 cctcctgaga ttctcttgaa atccgacttt gtcctttacc aagccggtac atggaaacga
    36241 atggatggta atcacggaag aatctccgca caatcgtgtc tggtcgggga aaacgtcgag
    36301 tcgggatcag ggtcgttccg gcggcacggc cagctcgcgt ggatggtcgt tccgctgccc
    36361 gtcacggagt cgatactgaa agtcccgccg gacagttggc tcaattccct catgctggcc
    36421 agacccagtc ctcgcattcg agcggacgaa agcgtccggc tgagattgaa gcccaagccg
    36481 ttgtcccgga tgagcaaatg gatccggtcc tcttcttcct tgaggcccaa ctccacggta
    36541 gtggctgtgc tgtggcgtac gatattgttc aaggcctcct gcacgatcct gaagaccacg
    36601 atcttcaggg actgggggac caggttctcg tcgatcggga aggtctttgc gatggcgatg
    36661 tcgggataga cctttgtaaa ctctcggcac agccacccga gggcagcggt gagtcccaag
    36721 tcgtccagga gagtgggccg caggctcatg tacaatcccc tcacctcggc aatgcccgcc
    36781 cggatcgtcg gcagcaactt ctccaaatgt tgaatgagat cccctttgcg gtcgttcagg
    36841 gccatgcgga tggccccctc gatcccgaat ttgatggcag ccagggtttg gccgctttga
    36901 tcgtggattt cccgggccag gcgcgttctc tcttcctcct gggcggcaag cagccgggac
    36961 gagaggaact tcagttgctg ctcggaacgg agaagggcgg cttcagcctt ccttcgctgc
    37021 tcgatttcca tccgcagggt ggcgttggcc cgctccaatt ccgccgtgcg ttcggctacc
    37081 cgggcttcga ggtcctggtg agcccgtaac aattcctcct cggaacgctt tttcaccagg
    37141 gcaacggcgg ccaattctcc gaaggccgaa gccagttgca catctcgctc gttgaagccg
    37201 ccgggcttgt cggccaggcc gaggagaccc accgtttttt cccggaggat caggggagca
    37261 aagagaacgt tctcgagggg catgtgtccc ggcggcagca aaagggaata ctcgctgcgg
    37321 gaaaagtcgt tgtcgtagat ggccttacga ctcgcgcacg ctcgaaggcg caatcctcgg
    37381 atgggcatgg gaaggtcttc gctcgcggtg cactctttgc cgccgggatc cagaaaagcg
    37441 acttccgcgc acatggtccg ttcgttcacc agagagacat agccggagcc cgcacccaga
    37501 agttccttgc agatccggaa aagagcccgg gccgtttcct cgaaggtccg gtattcgagg
    37561 acggctttgg acccggccag cagagccatt atctccctct gaagacgggt cgtctggtga
    37621 agggcttcct cgagttcctt ccgagctctt ccctcttcgg aaacgtcaat ggcatagacc
    37681 aggaagacgg attggccgac gggaatccac cacaggttcc agagtcggtc gcaaaggggg
    37741 aactgacaga gctgggtggc ccggttttcc ttgatggcct ggtccgccag gcagaagcga
    37801 cagatgtacc cttccccgga gagggactca cgagactcgg tcgcgccagg ttccggatct
    37861 tcggcacggt cttctttccc ggcgaaatgc cgccagcacg gctcgcccac cacggcgccc
    37921 atttcccggg cagcctggtt ggccgccagg accactcgtt tgcggtcaat caacatgacg
    37981 ggatggggaa gagaatccag gacaaattcg ttgaccccat agacagccat ggattcactg
    38041 agagacgggg aagagctcga ggcgcttggg acgaggagat ggttaccggt gtcgatcggt
    38101 tcgcttccgg aagcttttcg acgatccacg tttggttcac gatcccttct tggttacccg
    38161 catcttaaaa gcggcagggc ttgttgcgtt accttgccgg atcccaggcg aaccaatccc
    38221 agagggacat cgttgctgaa aagggtgacc aggatgaatt ccctgctgac ccgggcgaaa
    38281 tggatatttt gcctggttcc tttatgaaaa aggagggtga agtcggactc gccgatgagc
    38341 tgagccatgc gttcactggc cccaaaattg gccgccgaca gggctgccaa cggcaggagg
    38401 tcctccagag ggagacttcc cctctcgagg atcaggttac cggaagaatc cacaatgaac
    38461 gcatgggtca ctcctttgtc caagagctca ttgttgatga ttctctccaa ctgcttcgcc
    38521 gaatctcggg acaggatcaa atccatggcg gctcttcgat atcctcgatg cggaataaac
    38581 gcaggaacgc aggattgaag cctcggaaat tcaggaacgc gtttcaaaca tactgccgga
    38641 taaacccgtg tatgtcaaca attccgaccg gaactcgtaa ggctggcggc ggcattatag
    38701 ccaaaccctt cctcccgtca acccggggtg caggatacag gacggcaggt ggaaggaaga
    38761 atgccggaag aatccgcgat cattggatca tctggcccga gtctgatcgg aagggaagct
    38821 gcggcggcat tgcagccaga cccttcgatc cattcgggtc tccccgcctc ggtcaaagca
    38881 gggcggaata aaaccggacc cagttgtttc ccataatccc ctgaacggct tccgacgggt
    38941 acccgtccct tgtcatgcgc tctgcgaggc ccgacagccg agagatatct tccaccccct
    39001 ccgtagcgga aaagaacccg caaaagtccg atcccagggc gacgaactcc ggcccgaagg
    39061 actgagtcac ccagtcgata tgccgaaata cgtcctctgc tttcgccaca ccgtccaggg
    39121 ccagcatatc agggctcacg gagatcccga tgattccccg acgttccacc aggaccctga
    39181 cctggtcccg gttgagattc cgcggtatat tgcagaaaaa ccggaaacct gtgtgggagc
    39241 tcaacaccgg tccttcgaag aggtccatga ggtcccaaaa acaaggatcg gacaggtgag
    39301 ccacatccac accgcacccg gcgtccttga gctttcgaac cacctctttc ccttcccgtc
    39361 tcagcccctc ggggaaaaag accccgttgc cgtctccgat ccgattgctt cccatatgag
    39421 tcaaacccac cacccgaatc ctcaagggat caagggcggt gagatccatg tccaccagtg
    39481 cgtcggcgtt ttccaggaga aacaggacgg cgggttcgtg ctccggggat tcatagaccg
    39541 actggagttc ccgggagctg ccaatgggaa caaccccgtc caggtggtcc cgggcgtatc
    39601 ggaccaactg ctggaaacga cctgcggcgg attcttctcc gttgtattgg tccgcacagt
    39661 agagggccga gacgatgatt cggacgtttc ctcgcttcaa aagatccagg gtgacggtgc
    39721 ccgaagagat ctcggaaaat ctcctgcccg agtgatgttc catcatctcg aagaggacat
    39781 ccacatggcc gtccacgatg ggaaaagagg ggggaacaga gtgcagatcc ataaagttct
    39841 ttcgagaacc ggggcaagga accaggaacc tcaccccggc tcatcggctg ttacctgacc
    39901 cttatctccg aaccggagat tccgtcggag catgtcagtt ggggacatga catccagtgt
    39961 ggagcacctt cttttccact tcctttacct tcaacatccc catcacctcc tttcggcgta
    40021 tcattcagag gacccgtgcc gcaggatttc catctgctcc agcgttcgga tcgcgtcgac
    40081 acagaggacc atgaaccggt cccgactgtg actgctgccg tggacggggt agagtctctc
    40141 gcagatttcg gtcggggaat tggtcccatc gcaccaggcg atcaggtccc taccgaatcg
    40201 attgatttcc ttgacatcca ggcggtcctc caccttgcgg aacacaagga aagtctcccg
    40261 aggtgcgtag aagctcccaa cctccccatt tttcaggtcc ataataatgt ctgtcatggg
    40321 aaatgtgaac tctttcatca gaactcgccg gtcgcgttgg gggcctggca ggcggatccc
    40381 atggatatcc atggagaatt ctccggggag cgactcgtat tttcccgcct ccatgctcgt
    40441 ccgttcatac tcgatcacat cggggaggtg ggcaaagcga atcctctccc gttgcccgag
    40501 cctttcccgg cagtagtcgc cgaagtaccg gaaacaatcc tcgggggaca agctcggttc
    40561 gttgcccccc cggcgttgct gcacccggag catccagtgg aggaaaaggt gtgaagccga
    40621 ttcctcgagt tcatgaacca gcagcaggaa agaccgggga aagtaattga ccatgagggg
    40681 aaagtaggtt gccaggaggt tcaaatcccg cagagaccct gccgggcagg ggagattata
    40741 gaaactgctg aaaaggagcg ggtccgaatc gatgaggtct tcgtcccctt cgagccgtcg
    40801 tccgccgtcg aactcgatgc ctagggagaa ataagtgtcc acctcccgga ccagcaggtg
    40861 ggaataatgc tcgtagagct cggttccggg caatatggtg gccatttgaa tcaaagggtt
    40921 ggtattcccg accccgccgc tcttcaacgc caggaccagg gtcgcatcga ggtcttccac
    40981 ctcttctcgg ggaaacccga tgacgaagct cagggtcggg acgattccct cgtcggtggt
    41041 ctcccgtacc cgctggtaca ggatgctttc gtcgatgtcc ttgtgaataa acgccagggt
    41101 tctcttggac cccgaatcga tcccgtaaca catggattcg cacccggcgt ttctcatggt
    41161 cctcagaagg gtcgagtcca cgccgtccaa tcgcgagatg cagtaccagg gaaggcggtt
    41221 cagaccttcc tccatcatgc gacggcagaa ctcctcgacg taactcctgc gggcagtaaa
    41281 ctgatcgtaa gcaagcagaa agcactcggc cccgaattcc ctgtgaaggt gacgtatctc
    41341 cccgatgatc cgctccacgg aaaaggtgcg ggtcctgcgc ctccagatga gggactggga
    41401 acagtaggca catcggtgag ggcagcccct gcccacctcg agaatggcaa tgcttcgccg
    41461 gatattgcag gcgtcgcggt attcggacag gggcggcacc aggcggtaat cggcgatggg
    41521 gagttcatcg agggaagtca aaagatcccg gtcgggattt ctcacgacct ctccggattc
    41581 tcggcttcga taggtgacgc cggggatgcc ctctccatct cggccctggg agtaagagtc
    41641 caccagctcg gggaaagtga tttccccctc gccgcggacg acggcatcga ggaagggaaa
    41701 ggccgtgagg gtcctccggt ccacgaaaga ggcgttgtgg ccgcccaaaa cgatcttcat
    41761 ggaggggtcg tgcctcttga ggagccgggc gatctggatg gcgggagggt aggtggtgca
    41821 ctggacggaa aaccccacca ggtccggggc ttcctcgagg atcctccgtg cgcagtggtc
    41881 atacacggcc ggacccagag agacgagtcc ctgccgaatg gccaggggaa agtccagtat
    41941 cagggtctcg tggggactct ccttgaggac ggcggcgaga ttcagcagcc ccaggggagg
    42001 aagattgtac atgggttcca ggtaaaaggg cggaaaaacc aagacgatct tcatgggaaa
    42061 tggatggttc cgggccgatc acgcatccgt cccgtccgaa tcccttccca tcaaggcaga
    42121 gagagatttc ctccctgctt tgatgggaga ggtcgcagga gggtgagcag cctgtctgcg
    42181 ggcaagccct actctccaaa aacgctgtcg accaaccttt gaagcatcgg ggtccccttg
    42241 atttccgttt cgaacagggg ggtaacggcc cgtacctggt cgccgaaggt ctccgcgatc
    42301 ctcgccaggt gctcttcctg catccggatg cggttcaaca caaagtctgc ggcatctcct
    42361 cccacctggt ccttctggat caccccgttc acgacgaccc ctcccaccgg tattccaaac
    42421 tcatgaaacc agtgaatgaa ccgcgtgatg acggcaatgg gcaggctttc ggggagggtt
    42481 acgaaaaaga aggccgtaag ggcctcgtcg gtaagcaggt ccttggcgtg ggccatgcga
    42541 tccttgaagc ccagcaggta ttccagcaga gggtccttct cttccttttt cttggaaaag
    42601 gacagcattt ccctcagaga cttggcctct tccctactgc gcagcatttt ctccacccaa
    42661 agggagtaca ccttggacat gcccaggagc ctccgggcat tggccgtggg ggccgtgtcg
    42721 aagatgtaaa attcatactc gtcccgaaac atcaggtcca tcatgttttc aaacatggcg
    42781 gattcttcga aggcaggatt catggtggcg gattcgatga actcgtccgc cttggtggtg
    42841 atgtctgcaa atttcaagaa ccagttgatc ttctcccgga tttccctctt ggacctttcg
    42901 atggtatccc gggtgtcgat ctcgaaggca tagcagttgg attcgtcaca gacgaccgtg
    42961 ggctttccga atacgttggt cccgaagaga ttggacaggc tgtgaaccgg attggtcgag
    43021 gcaaggagcg tctttcggcc ttggcgagcc gaccacaagg ctgcggctcc cgccatgacc
    43081 gtcttcccca caccgccttt tcccccaaag aagatgtact tcattcgagg gtggtccttc
    43141 atgaactgat tcatgctgtt ggtgatctgg atcacggaaa ccccctctgc gctcttagaa
    43201 atcgccgtac atggatcgag ccatcttctc aatcatcgca agcccggtca catcccgttc
    43261 catttcatgg accgaggcga gaatctgccc cgaaaaatcc gtttcgatga ccttcaggta
    43321 gtgctcttgc ataacgaaac ggttcttcaa gtactccggg atgttctttt ccttcagctc
    43381 cgagggcaaa acccgattga tgatgtatcc gctgagggga acatcaaact tggcgaacag
    43441 ctcggcagcc ttctgggtgt ccttgatcac catttcttcg gctaccagca caaaaaagaa
    43501 agccgtcttc tctttgtcgg tgaggatgct tgaggacttg ttgatccgtt ccttgatgta
    43561 gaggagctcc ttcagaatgg cgtcttcttc ggattccttg tctcgtcgaa tcaccgcagc
    43621 cacgtggtcg tattcgctca tttgctcgcg aagcttggtg atcttatcga tccactcgtc
    43681 gtagacggaa gccatgctga ggtagtacag ggcatgtccc aggggaacca ggtcgtaaat
    43741 gtagtaatca tagcccccct tgaccacgat atccaccact tcgtcgaaaa tagcgctttc
    43801 ttccatggcg ggttccgccg cagccgcctg aatgtagctc tcgatctcgt cggggatctg
    43861 ttccatccca tacatgtcga ggatcttgtg ccgaatctcc tcctggtact ctttgacccg
    43921 ccgatcggca tcgatttcct gggcatagag gttgggcatg atttccacgg gtcctttccc
    43981 aaagatatcc cgctggaaaa tgtcgctgag ggaagcttgg ggatctacgg agaagaccaa
    44041 tacgcgcttt cccgttttgg caaggtaata ggcggtcgcg gcggaaaacg tcgtcttccc
    44101 caatcccccc ttgcctccga acataatgta cttgcgatcc ggatactgtt ggaagatgtc
    44161 tgcgagtgaa ataggagacc ctcctttcta cctggcaggt gtcggctatg ggctctggac
    44221 gggaatccgg gccggggcgg gacggccccc ccaaggggaa gcccccatcc ttgccgactc
    44281 gggatcaagc cagtgcgtcc gtgagatcct tttcccggag cattcggcgc ttccacgtgg
    44341 aaatctgggg gaccacgtaa ggaaggagcc tgagggaata atagggggga agaatgggaa
    44401 cccccagcat gtcccccatg gtgatcagga tgaaaaggtg ttccatgctg gccctggttt
    44461 tcaacgcaaa acgggcggaa tcgtgggcgg ccaccccgta cacgaattcc ttgaccgctt
    44521 gaaagagacc gccctttttt cccttctcgt ctttggacat gccgtttcct cctctcctac
    44581 ggcgccgcag ctcgtcaagc accccccgat cccgggcgga tgcgatcctc cacgggaacc
    44641 gaccgggctc ccgcatcttc tcgattcttg taccgcattc ggcaagctgg tgtccaccct
    44701 ccagtttcga tctcaaggca ccggccgggc ggttcagggg aatccccacc gcgagctccg
    44761 agagcgacgg gcaacgggcc gcctcgcatg gaggcacgga cccccgaaac ggatcaaggg
    44821 gcattctccc gaaaagccga atccccggtt cctgcaatga ctccgtcccg cacccgttcc
    44881 ggggcggccc gcaggcccgg tccatgaacg ggcccgggaa agggttggtc taggcggacg
    44941 cgggctgcat cctgttggcc cggtatcgtc caaaagcttt gaaggcttcc actgccagga
    45001 tgatcgccgc gatgaccagg aaaaagccca cgatccccat gatggtgttc ccgatgaggg
    45061 cctccccctt cacgacgccc ctgaagacct tgtccaggag ggaataggaa gtgtacagca
    45121 gggcccccac cgtggtgacg aacatgaaga tcatgggata gaaggtccag gcggagctcc
    45181 tcccctcgga catgagccat gcggtgatga gcatcagggc aagggatgcc atgagttgat
    45241 tggctccgcc gaaaagcacc cagagatact gccaccatcc ggtcatgacc aacaccaggc
    45301 ccagcaggct tgccacgatg gttccgatat gagggttctt gaaaaggggg ctcacgtccc
    45361 caaggagttc ggaggtggcc accctcatga agcgaacggc cagctgcatg atggtgatgg
    45421 ccagaaagat catcatgacg ctgccgtaag acttgccgat cccggccgaa atccccaggg
    45481 ccgagagaaa cttcgagacg ccggcagcga acaccccggc gggccctttg gccacggccg
    45541 ccccgtattc cgaagccgag gcataaatgg ttcccgcgat gatgagggcg aataccgcca
    45601 gcatcatttc cacgaacatc acgcccgctc ccacgggcct tgcatccatc tcgttttcga
    45661 gctgccgggc cgtccccgag ctggacacga tgctatgcca tcccgagatg gctccgcagg
    45721 cgatggtcac gaacataatg ggccaaatgg gaccgatctt gatggtaaag ctcgtataag
    45781 cggggagcgt aaagtccggg tggaggatga gcacgccgat gacccccccc agcaacccga
    45841 aaaagacgat gtaggaagcc acgtagttga tgggtagggc aaaacgccag atggggagca
    45901 ccgcggcaaa gtagcagaaa acgaaggcgg cgatggccca aagggcacgg ttgttggaca
    45961 aagcctcccc caggatcttg tccgatgggg cgacggttcc catccagatt cccaccatgg
    46021 cgatgagcac ggtgacgacg gtggtcagga tgatatcctt cctccaccgg tagatcatct
    46081 gtcctgccag gatgcctccg atggtgagga acagccatgc catgggagaa gcttttaagt
    46141 tgacggcggt gctgaccacc acgtttccga aggcgctggt gatgagtagc aggtagaagt
    46201 agatgaaaga gagcaggatg attctggccc tgggcgagat tagccgatag ctgaggcccc
    46261 cgaaggacgc tccttcattg cgcatggcga ccatggtact ggcatagtcg tgaacccatc
    46321 cgatgagcag cgttcccacc aggatccaga ccaaggcggg caaccacccc cactggatgg
    46381 cgatgatggg ccccactatg ggaccggctc cggcgatgga cttgaactgg tacccaaaca
    46441 gaacgttctt gctggtgggc atgaattcca ccccgtccat gtacatgacc gccggagtcg
    46501 ccctcttggg gtccgccttt atgatgttgg tgtccacata ccgggaatag aaacggtaac
    46561 cgatgaaaaa cactaaaaat gccaggacga gtactaggac tgaattcatt tccaaacctc
    46621 ctgtgggaaa atgatccaaa actcatggta cccgaacact ctgagaggtc aactcgtcga
    46681 atcggttgat tcacccaccc ctgtatcgag aacggaaaca caacggccga accatagccg
    46741 cccggcccga agattcggct gtccggtcga tacgaaagca cggtcgtttg tctttcagct
    46801 gctgcaagta ctggagaggc gtccaaggcc tcaggacgag cttcccgccc gggccccccc
    46861 agcagcacaa cccgatgaat cctagatcaa ggaccggtag aaacgtctcc tcccggagaa
    46921 aggcgtccct cgaacgaaag catcacgata tcacccggga aagtacgaac atcgctcatc
    46981 aagggaatcc gcctctcccg tttgaagctc gggcgaagca cttcacccaa tgaaatggat
    47041 tgaatcatga atccatgact ggtggatgat ggatgaggaa atttcatggg atttgccaaa
    47101 agaggtttga tttgtgagca aacactcccc agcgcgacct gacgatcttc ccaaaaatat
    47161 cgcacggatg caataagaat ttgcggctct tgagcactgg gcaacccatc cgaggccggc
    47221 gagaccgggg attggtcgag ggattcttcc ggggacccga gacgaaagac tccccttatg
    47281 gacctccctt ccacgggaaa agtccctcca cccgggaatt tgcttgtgac atttccccaa
    47341 ccggcatatg gttccgtaga cttgtcccga ggatgcatcc aggaaccgca cccaatcagg
    47401 aggaatgaac gatgacaacg aaacagtggg atagcccgcc gtccatgggc atcgattcgt
    47461 cgaagctcta ccgagcaaaa ctcgaaacca acaagggggt catcctgctg gagctctacc
    47521 cgcaatatgc cccaaaaacc accaataatt tcgttttctt gtgccgggaa ggtttctacg
    47581 acggagtgat ttttcaccgg gtgatcgagg actttgtcat ccagggcggg gacccgacgg
    47641 gaacaggccg gggaggcccc ggctaccggt tcgaggacga aacccgggga aatcccctga
    47701 aacacgagac gggggtcatc tccatggcca acgcgggtcc caataccaat gggagccagt
    47761 ttttcataac ccattctcct cagccccatc tcgacggacg acatacggtt tttggaaaag
    47821 tgatggaggg aatggaagtg gtcaaggccg ttgagcaggg ggaccggatc ctgaaagcgg
    47881 aaatcgaaga agcctgatcc cctcctgccc tacacgctcc cccaccccct cccgcccggg
    47941 gagggggcga cgtgtttccg ggcagctcct cccgcccgtg ggtccgcagg aaccgtcctg
    48001 ccggccgcag ccgggccttt tcacggaact cgacccgccc caaatttgcc gatccgcttc
    48061 gcccaccgtt ggggcatccg tttcctgtgg ggtccgagcg tttgccggaa gatcccggga
    48121 ttgacgatga gcgcgggaac ggaacggtgt ttgatccccc tttccgcggc ggcccttcag
    48181 gagggggaaa aaacaaggcc cgtacgaaga tgtacgggcc tcggagtccc tcaattccgc
    48241 agggtggcag ggcccctcct aggcggtttc ctccttgatg gtggtggcaa tcttgtacaa
    48301 cacggacaaa atgaagaatc ccgttgccca gactcccagc acgatcatca actcgggaat
    48361 ggtgggggag tactcgcgca cgtggtcaaa ggggttggga atgaagcctg cgatgaccaa
    48421 gcccatgccc ttctccaacc acatggagag aaacaccgca gcgcaggcaa tcttgagggt
    48481 cttctcgttc ttgcgggtgt tgggattgat gagcaggacc agggcgacca ccgcaaggat
    48541 cgcgaagagc cacatgaacg gcaccagggt gcgatgtccc tcaagcccca cgaagaggta
    48601 cttcaaggct tccatgtggg aggggatctt gctgtagaac gccgtaaaga actcgagtcc
    48661 cacgaaaaac acgctgatga ccatggcgta ggtggcgatg atggaaagct tgtcgatggc
    48721 ttctttgccc ggatcgaact tggtgtattt cttcaccagg tacgccagga ggatgagaag
    48781 ggccgggccg gagcagaagg ccgaggccaa aaagcgggct gccatgatgg ccgtcaacca
    48841 gtagtgcctt ccgggaagac cggcgtacag gaaggccgta accgtgtgaa tggacacggc
    48901 ccaagggatg gaaaggtaga tgaggggctt cacccagccg gggtaatgaa cccccttgcg
    48961 ttcggcctcc aggacgttcc acccgatgat gatgttcagg aagagatagc cgttcaacac
    49021 gatcatatcc caaaagagga tggagttggg tgtgggatag agaagcacgt tgaggagcct
    49081 catgggcttc ccaagatcga cgatgataaa cagcagacac atggtgacgg aagcgacggc
    49141 caggaattcg ccaaggatgg tgattctgcc gaagaccttc acgttgtgca ggtagtaggg
    49201 caggaccacc atgaccgcgg aggcggccac accgacgagg aaggtgaatt gggcgatgta
    49261 gaacccccag gagacgtccc gactcaaccc cgtgatggtc aaccctcggt tcaactgtac
    49321 cagatagctg gcaaatccca tgcccatgag cacgagcaga agcatcagcc accggaagta
    49381 tctttgatct cctttaaatg ccctctcaag cattgctgcc ccctgtccta ggatacgatg
    49441 taatagacgt tcggctcggt accgagctcg ggtttgcgtc gaatggtgta gtttgatgca
    49501 agcagtttgc ggacgttgga cttgggatcg gccagatccc caaaggccag gcccccgacc
    49561 ttgcatgcct cgacacaggc gggcatcaat cccacctcca gccgctcgga gcagaactca
    49621 catttctcca cgacccctcg ttcccgggtc ggatacgcca tgttgagctc gggaatgtag
    49681 ggacggggtt ctcgaaagtt gaagcttcgc gccccgaagg ggcaggcggc catgcagaac
    49741 ctgcatccga tgcatcggtg catgtccatc atggtgatcc cgtcggcacg cttgaaggtg
    49801 gcctgggtgg ggcacactcg gacgcaggga ggattggcgc agtggttgca caatgcaagg
    49861 aaaggctttc cccgcaggtt ttcgatgttg tacgggttgc cctggccggg aaagacgttt
    49921 tcgtagttgt ccgtccagag ccacttgatt tcctctttga cattccccat ctcgggcaca
    49981 ttgtggacct tgtggcacgc atcgatgcag tccttgcagc cggccttgcc cttctcccaa
    50041 cactttctca tatcgacgac catggcccac tgtttgccgt ggaaggcttc ggggttcgag
    50101 gccacccggg gcaggtcggt tttcgcaagg agttcatgaa tcggcagggt tgccagcccc
    50161 agggtacaaa gtccgccgat tttcaagaag cttcttcggt tggtttccat tacttggtct
    50221 ccttcggggc aatatgacaa gtccagcaat agggcgttac gttaagctcc aatcccgcgt
    50281 acacgtggca ttggtcgcaa aactgagact tgttggaatg gcagcggacg cattcgttct
    50341 gaaggctcat gttgtactgc ttgccgtccg ccgccacgta gattcggaac ccgtcccgca
    50401 cgaccgaggt tctccagtcg ttgagcagct gcatgtgtcc cgtacgcatc tcgatcttcg
    50461 gcaggacgca ctgtttgacc gccatctgtt ggatcacggg ggtatcgatc ttggggtccg
    50521 gtgccggagc agccttgccc atgttgtacc acagggggaa agtggctatg cccagaaaga
    50581 tgatgattcc cgggatgatc gtcttcgcgt tatagatcct catcgtcgct ctcctccatt
    50641 cctgcgagcg gctcgccgcg tagatccgtg gttcgctctg cttcgccctt catcaccagg
    50701 gcgtttccga ccagctcgtg aacgccggtg acccctactc cgggggccca atagttcaga
    50761 gaagtgacaa ggaccgcccg gtcgatggcg caaatgcagg ccatcatgtt cacatcatgc
    50821 ttttcctgga cgtatcgggc cgcattggcc cgggggagcc ccccgcgcat tctcatttcc
    50881 atgaattcgt cattgttgag gccggaaccg ctcccgcaac agaaggtttg ctcccgggtg
    50941 gtctccggcg gcatctcgaa gaagttgttg cagacgctct gaatcacgta gcgcggttcc
    51001 tcgaagaagc ccattccccg agacggattg caggaatcgt ggtaggtgac ccggagatgg
    51061 tcgttgcggc tggggtccag tttcaacttg ccgttccgga tgagatcggc ggtgaattcc
    51121 acgatgtgca ccatcttggt ggacttggcg ttgtcaaacc gggtgccggt gatgggggac
    51181 acgggttctt ccaggaaatc ggcagggccg ttcatggtgt ccatgtactg gttgatgacc
    51241 cgccacatgt gcccgcattc tcccccgagg atccacttga ctcccagccg tttggcctcg
    51301 gcgtacatct tggcgttgag cctcttcatc atctcgtggg aggtgaagaa accgaaattc
    51361 ccgccttccg atgcgtaggt ggaccaggtg tagtccagtc ccagctcgtg gaagagcatg
    51421 aggtagccca tgagggtgta ggtccccgga tcggcgaaca cgtcccccga cggggtgatg
    51481 aacaggatct cggcgccctt tcgattgaag gtcggctcga ccctcacccc ggacacggcc
    51541 tcgatctcgt ccacgaagaa ttcgatcatg tccttgaagg cgtgaggctg aatgcccaaa
    51601 tggttcccgt tcatgtagca gttggccacg ggacccacga cccagtcgat gttgagcccc
    51661 accaagttga ggagctcccg gccgatgatg gtgatctccg ccgtgtcgat gccgtaggga
    51721 cagaatagcg aacatcggcg gcattcggtg cactggaaga agtaatagaa ccactccttg
    51781 aggacatcca cggtgagctc ccgggcgccc gcgattcggc ccaacacctt ccccgccgtg
    51841 gtgaaatcct tgcggtagac cgagcggagg agttccgccc gcagcacggg catgttcttg
    51901 ggatcgcccg aaccgatata gaagtgacat ttgtcggcac aggcaccgca tcgaacgcag
    51961 atgtccatga aaacccgcaa ggaccggaac cgctccagcc gttccctcaa tccttccaga
    52021 atgatctctt tccagttatc gggaagcttc cagtcctcgt ccatggggga ccattcccga
    52081 gggttgggca atcccaccac ctcgaggttc ttcggttttc ccgggtaaga aaaggtgccg
    52141 gggcgaaaaa ccacgggggt gtccatccag ccggtctggg gcaacttatg atcaatgaca
    52201 agctcgttgg gttttgggac cttagccatg tttactcctt ctccactggt aatccggcct
    52261 caatcatctt ctctctgaaa tggtcttcat actcctcgta cgtgtgaacc ttgacggggt
    52321 aattccacgg attgacgtgc cgcttcatac ggctgtcatt ggggagattt cgcgtgggac
    52381 tcaggaaaac ccctcccaga tgcatcaatt tgctgaaggg aaagtaggca aacaacgtac
    52441 tgaagagaaa aagatgaata aagaagagaa cgccgatgcc gtcgggcacc ttgggttgca
    52501 gcgtcaccag gctcatggcg agctctttga cccgaaccac gtcgaccttg agaaaatagc
    52561 gcatgaggat gccggtaatt ccgatcccca ggaggacgaa gaggggaaaa tagtcggcgg
    52621 gaagagaaat atagcggacc tgggggatga tcactctgcg aatgaacagg taggtgaccg
    52681 cgagaatgaa aatcgcatcg gtgaggtaga ccccggggag cccgagctgg agcatcccgt
    52741 ccagccgatc ggtcatgagc acaaaaaagg gcacgggctc cgtgaagaac cgaaggtgcc
    52801 tcagaaggat cacgaaaaag caccaatgaa agagaaggcc cgccagccag agccatttct
    52861 cccactggta caccaccttg ggaccttggt gcagctcgaa cttcgtgttc ctgaaaaggg
    52921 aacgaaagag caggacttcg agaagcattc tgcccacgac ccccgccgtg gagctggggt
    52981 tgtcgagctt gttttgcttc acccacggga gcgacttctg ctgcccggcg gtggttggaa
    53041 tacggaaggg gacgggggat cgagcccatg taatcacgcg gtaaaccact cccagcagga
    53101 agatgaaaac cgccaggtac gggatgatca ccccgaaaag gtaagtcatg ccggccgctt
    53161 gcacccccag aaaggcggcc aaaacgagcg caatgactac ggtcaatgaa aacttgattc
    53221 ccattgaaca atccctcgtg tcaaaagagt tttgtggtta agtgttgccc ttcggcccct
    53281 ccgcccgaaa ctcggggatc tcggcagcga gacccgccaa ctgaaggagg cggaaggtcc
    53341 ggttcttcaa ttcattgact ttgagctcgt acaacttttc acgacactgc atgtacacgt
    53401 caaacgccag cagggaaatt tgatcgatca aaaggtcgaa agacgccagt tcttccgaag
    53461 aaacctgctg ggtttggatg tgctcctgga acatgtcccg aaccaacccc ttgggcaaaa
    53521 acatgaacgc cacggcctgg gaaggcgaaa aatcctggac cgctcgcacc cggatgatgc
    53581 ggtccagcca gggcctcagc ttttccgagt ccatgccccg gagcatctcg ccgtagaggc
    53641 cgtcgagccc atgataaatg gtatggccaa ccggattgac gaactgattg gattcttttc
    53701 cgagaaatcg tgccgtttca gggggatagg ttccgaccaa cgccccaaac cacttcttga
    53761 cgaatcgttc tctgtctctt gcgagcaaat cccctaggtt catctggaga gatccctttc
    53821 cctatcctgt gcgacgtgat cgaaagaccg gtttccccga tcgcaacctg accatccccc
    53881 ccttccggca aaaaatcatg cgggcgggaa accacttttc gcccgcatgt cagaggccgt
    53941 caatcgattg cccaagcaga aattccaata ttgtgaatgt cggcacgtta ccccaatccc
    54001 ctcccggtgt caagattgaa actggcggga aaaaggcccg ggacggaccg ccccccggcg
    54061 cccccaaaaa gccggaaccc tacggttttc ccccctgccc ggaggtcgct cttgtgcgta
    54121 accctttggc atccccccgg cgccgtcctc cggggattcg tgggccagtt ccccggatgg
    54181 ctcttgattc caaccccgaa gtgtggttct ttcccttgag aagctctttc tcgttgaaga
    54241 aaaggacccc cctgccggca ctcgtttccc tgcagcgcgt ccccttcggc tccgccccgt
    54301 acccccggca tgaagaaagg aaagaggtct atggcgaagt accgtggaat acatcacctg
    54361 gccatggcta ccaacaacat ggactcgacc atccgcttct ggcgggatct tctgggaatg
    54421 aggattgtgg ccgggttggg caaacccggg tatcggcatt actttttcga gatttcctcc
    54481 gaggacctca ttgccttttt cgaatggcct cacgtggaac ccatcccgga aaaagaccac
    54541 ggctatcccg tctccggccc gttggttttc gaccacgttt ccatcggtgt ggaaaccgaa
    54601 gaggatctgt gggagctcaa agacatgctc gaggctgcgg ggttctgggt gtccgaggtt
    54661 atcgaccacg ggttcatcca ttccgtctat tccttcgatc ccaacggcat ccccatcgag
    54721 ttcagctgca ccgtgccggg agtcgacgtc cgaagacgtc ccgtaatggt ggattcggcc
    54781 ccttcggagg taaccctcga gggtcccgag cctcggccgc aatggcggcc tcccgtgacg
    54841 gagcccacgg ccgaagtcga ccgacgggtg tatccggggg aaggcgtgga cctggtcgag
    54901 ggaaagaaaa agaactggtg ggaagaccgg gtgcatttac cgggtccaca gccccagccc
    54961 tgatcggtcg tcggcaccac gcgaaaaagt tttgggatcg ggaatcaggt ccctccgttg
    55021 cccccatccg tgtgctgaag gatttcgggg aaggggggag gcaaaagagg cccgtaccag
    55081 aatccctcgg cttcatacac tcgccatggc tcgtcggagc ccgtcagcca aaagaggagc
    55141 aacgagaggt cctcgtcgat gaagaactgc cgggtcacct ccacaatgag gggttgggac
    55201 gggtcgtcca ccataggcca atcttccggg tttcccgtat agaggtacca ataccagccc
    55261 tccttcttgg gcttcgtctt tgtccactcc atgccgtgat cctctctccg ttgcggtgca
    55321 tggctcgcct tgtcgcatac gctgggggtt ctcgtgaaaa aggcacaaga aggccaactt
    55381 tcctatcaga acaacgggaa gaaatccacc gcgaaggacg aaggtggatt tccacccgga
    55441 gtgtccgtgg gcctccgggt tttcctcaaa gaagggaggc atcggcagcc aataagcatt
    55501 ccaagacgca gcattctgtg cctgatcggc ttgatccttc taatcggctg tgtgccggaa
    55561 tccgacaacc ctctctcggg tccccggtcc gtccaatggg atcaaagtgt cacgggtacc
    55621 tgggccgggc gtgttcggga gtcaaacgcg acggtctggc ttcacatcgc accggatcgc
    55681 gatcccccgg cggactcgag catgattttg accgaagaag ggaagggcat ggagctgttc
    55741 ttttaccgaa tgcatccttc cgaggtaggt ccgtaccggt tcatgaatct gaaaccggca
    55801 tatcccgaga accctcgagt gtcgaaagga ggagaacgac tccgggacgc aaaaggcggc
    55861 aacctacccg ggggcaaaat atgaaatcag cgagggtacg agggtacttt gaaagtccgg
    55921 ctcatgaccc gggaaacggc ccgatacgtg gagagcggcc agatccgggg agaagtgaag
    55981 aggaacaaat acatcaccgc gacaaggatc accgacaccg gggaaaatct cgcgaagatc
    56041 ctcaaaacta cggatcacgg ctccattttc gagctctttc tggaaggcag gcgggtcccc
    56101 tggacgcccc ccgaggcctc caatccgcca aagccctgac gagaccgcac ccaaggggat
    56161 gcccgagatc cgatccttgg gcggagtctc gaggggtttc catccgcccc cccggacctt
    56221 cccgtcggtg ggaaaacaaa aaagggccgg cggttgttcc cgctggccct ttggcttttc
    56281 tggtggagct acggggagtc gaaccccgga cctcttgaat gccattcaag cgctctccca
    56341 actgagctac agccccacgc acacgtgtgt cgtccgcgcc cgcaagtgag gagcggtgca
    56401 gcgaaacgtc gtatgtgtaa cgaatttgcc ccgggctgtc aagctcaatt ccaaatcggg
    56461 caattagcct tcagtgttta accattcgcc tcgaatttcc tgtggttgag ctgctttggc
    56521 ttccattcaa caggtaacgt ccgaaacttc tcacatacca tgccaaagag ccaacagcga
    56581 ggcgctcaat ttcgcctgaa taggattgat cccggcttgg acgaccggac cgcagtggga
    56641 aatcggcggg gtggaacatc cgccgggcaa ggtccatggg ttccatttca gggaagttcc
    56701 atgcgaccgc aaggggcgat gcagggaggg atgcgccggc agcctgaacg gtggccggga
    56761 gcctcctccc aagggttcac acgggcaggg atgcggccac tgcacctagg tcatttccct
    56821 tgacacctcg gaaaccccct gtgtataaac cccctgttgt gccggagtgg tgaaaccggt
    56881 agacgcgctg gactcaaaat ccagtgggcc ttgcgcccgt gagagttcga gtctctcctc
    56941 cggcaccaga aagaccctgt aatttccaac acctccattg ggatgcgccg gaaggttcgc
    57001 cccccttttc ttgtcaagtt ccgcccttgt gaccgtttcc gtgaccgttg ttgcccgtcg
    57061 ccctgccagg taccgtttgt gtttcgaggc ggcctggcct gcatgttcct gccgtccacg
    57121 gcggaaggat tcatcgcctg aggttctgcc catgcgggaa ggggtttgtc tctttgatgt
    57181 ttcactccct gtccgttatg atggagctgt cgaatcaacg gcagactccg taactcctac
    57241 ccctttgata tggaagccct accgattcct ttcgctgccc ttggtggctt gatcctctct
    57301 caagagatgc actttgtccc gaaaaatgca gtgcctctca gcggcctctt ttgcgcggga
    57361 aaaggagtac agactatccg agaagcaggg ctcaaactat ccaagggcga ttgggtgcta
    57421 cacccccgga tgtccgggtg gggttacggg aaggtcttgg tagtgatggg cgagcgcaag
    57481 gggcgtgtgc tcttcttgct tgcaggggag aagactctgt cacgggaaca cgccggcctg
    57541 aatcttgtgg aaggggacag ggtatcccag gctgcattca acgaccagtt ggcggaattc
    57601 aagctgaacc tcgccaggga tctgctcagc tccgaagatc ttcgccgggg aatgacctgc
    57661 agtccgtgcg aatggctcac ttccaaacgt gagttgtgca agtccggtcc ggacaatctt
    57721 cgcatctaca gcctcacccg gaaggggaag atgctacgag ctcattatcg atcggcagga
    57781 cgggatcata tacgtgcagc cccgcaaacc ggcgaagatt ggaggaccat gtgctcaccc
    57841 gccgggagat ggcgcgcatc tgccgcgaga taggatgcaa aaaggttctc ttcgattccc
    57901 gggacctcga actggacggc ggctacacca cgcgaagcct gtatgaattg ggacccacgt
    57961 accggtgtga cggcatcggc ctccagatct ccatcgccca tgtcatgccc cgacacgccg
    58021 gggttcgaag ggaaatccgg tctctcacca ccgttgccaa gaaccgggga acgtgggtgg
    58081 aatccttcga ggacgtttcc gaagccgaga aatggctgag gggtccccga aacggaaagg
    58141 gcttgtgatt gagcgtgctc tatgattgta tcccagacga agcgatcggt tcatggaccc
    58201 cgggtgcggg cccgggccaa ccggttcggc cccccgggcc gtgggggatg gaagcgggtc
    58261 gattccaaac agcccgttcg atcctcgatc caattgccga ccgccgcctg tttttcgccc
    58321 cgggcaagag gggtgtggtg aaaatggtag cctgagctgg gatgaactgg gagaaggagg
    58381 atacaacgat gccgaagatc ggagtgcttc tgtcgggatg cggagtgtat gacggatcgg
    58441 aaattcacga ggcggtcttg acgctcctgg ccctggacag ggcaaatgcg cagattgtat
    58501 gcatggcgcc caacgtggac caggcccatg tgatcaacca tctcaccggt gaggtgaccg
    58561 gcgagaaaag aaacgtgctg gtcgagtccg cccgcattgc ccggggggag atccgggacc
    58621 tcaaggacgt gaaggcggcg gacctggacg ggttgatcat tcccgggggg tacggcgctg
    58681 caaagaacct cagcgacttt gcgtttaagg gacccgatgc cgtggtgaac ccggaagtgg
    58741 agaggctgct tcgggagatg gttgccgcga ataagcccgt gggggccatc tgtattgccc
    58801 cggccaccct gaccaaggcc ctggccgaca aaaagcccga agtcaccatt ggaacggaca
    58861 aggatacggc cgcggccatc gaagccatgg gaggacaaca caaggtgtgt tcggtggatc
    58921 agatccacct ggatgccggg aacaagatcg tctcgactcc cgcctacatg ttggggccag
    58981 gcatcaagga tgtggccgag gggatcggca aactggtgga aaaggtggtg gcgctggcaa
    59041 aatagggacc gggccgatcc ccgcgtttcc ccgcgtttca tgcgaacggt ccctcgaaga
    59101 aagaaggacc gttcgctccc gcctttcaat ccgccttcca gcggtggtac agaacattca
    59161 ataccttcac aaactccccg gtagcctttc ggtttcccac tcgattggga tggctgtcgt
    59221 ccgagcccga gggataagcg gcgacgttgc ggttgaccgt gtggatgtgc tgcagcgctc
    59281 ccgcccacca tcggtgatga ttcccccggg ccttgcccag gtcggtcgtg cggggatctc
    59341 ccccgttgct tgtcagaacg ttgtagaagt cgaacacccc gacattggag tggggatagt
    59401 ccttcaacca ctcgttgacc agccacaggt tgaaggcccg ggcgttggcc gcttgggcgg
    59461 cggaggtttc gttggggttc aggggagggg cggtgatcac cacgaagagc ttgtcccggc
    59521 gagtctcgaa gtactggaga atgtcgttgt agatgccttt ggcgtaaccc accttcatgt
    59581 atggggagta agcgtcctgg cctcgaaggg gattctcccc ggcgggtggc tttgccgtgg
    59641 gctttccgcc gaggttggag ttgggaaagc aggacttgaa aagaatgatc tcgttgggtc
    59701 cccccgggtc ttgggacagg cgcgagtagt cgcagtgctg cccgctttcc gcgtagagag
    59761 ccttggtata cctgctgcga ttgggaccgg caaaccagtt gtaccagtgt cccaggtccg
    59821 tgtggtcgcc gatgcgatcg aacccgacgt ccaggtcccg tggcccccac ccgtagttgg
    59881 tgtcgctgac gaaatagttg ctgtttcgaa gggcgatgcc cagccgtccg ttgcagtctg
    59941 ccaaccagtt ttccccgctg gaatgatgaa tgaagatgag ttttacggga gaggaaggcg
    60001 gcttggggtt gtcggcggcc gggacggctc ggggtccgca gagaatcaaa aagaacgcaa
    60061 tcgtcagctg cagaaccggc ccaagcggtc ggggaagaca agacctttgg gagaaaagcc
    60121 gtaggagtgt catggaatcc tcctctgttt tcgggccttt tcccctccgg gttgccggag
    60181 ggggcaatca ggccggtagg aagcctttgc cccggcaagc gctgcagtcc agcttaccgt
    60241 ctccctcaca cccggggcat tcccggggaa gaggcagatc ttgccagggt cccagaaaga
    60301 acgcaggcag gtgaaaagtg ccgatacctc gacagcggga gcagggaacc gtcccgcgac
    60361 cggaacaggc ggggcacgac cgcctcgagc cgggacggtt ggggacggac gtcataaggc
    60421 atttcctgga acaactgaga atctgaacag atggaagagt gcgggctcat ataggtcgag
    60481 gaaccgaaac catcctacca cacagaaaac cgcggtacaa tagaaactcc agaggaaggg
    60541 ttcagagaag ccgcgccgcc ttcgctcaag ggcttgcggg atcccctgcg ccgggcaggc
    60601 agtcgctggg acccggggca aagatgaaag tccccgggtg accacaaacc ccttccgatt
    60661 atctccggcc atcgctttcc atggcctctt cggcattctt gctccgattg agagggtcaa
    60721 ttccggcgag aatcagaaaa ccgcccaggc aaaccgcgct gattccgggc atggggttgt
    60781 ccgcaatttg aaaaaacccc gtagccccga cgatcaatcc caaaagaatc cggcgtgaga
    60841 tgctcatatc cgcctccttt gttcgttccc tcggaaagga gagcaacagg cgtgccggcc
    60901 cgcccggggc tcgatcctca gacgggttcg gcccccccgc cggcaggggc cttcggcacg
    60961 ccttgttcac cgttgggtcc tggggagact cgggcctcac cgcaggcagg gggcaaatcg
    61021 gctcgaaaac cgacctgggt tgcgtacgat atttccatga ggtgacgaga tttcggcaac
    61081 gggcccggga cccgggaatc cgaaggacct agcgggaaaa ctcctggggg tcccctcttt
    61141 ccttccttga caaagaaaag gaacccgaat agaaatagac cgatcggtcg gtattataag
    61201 agaggcccgt ccccggtggc aaccaaggga aaactcacgc gacaaaccat catcgagaaa
    61261 tccattcaac tcttctcggt caagggatat ttccacacct ccattgcaga catcctggaa
    61321 gcggtcggca tcacccgggg agggctctac gggcacttcc gaagcaaaga ggagatctgg
    61381 tacgccgcct accaggagtg cacccgtatc tggaaagaca tcgtgtttcg gggagtcaaa
    61441 gaagctccgg accccttgga gcgcattcgt cgggtcatcc gcaacagcat gcaggactac
    61501 ctggggggag gggtcttcga gggaggctgc tttttgttca attccctggt ggaattctcg
    61561 agacaatcct cggccatgaa ccagcacatc ctgcaaggat tcagggggtt ttcccgcctg
    61621 ctgcactcct ggctcgaaga agcccgtcaa atggcaatgc tcaaggaagg actgcccctg
    61681 gaggagatcg ccaacttcat cgtgatctcc ctaaacggag cgggtccgtt gtatacttcc
    61741 agcggagatc ccgccgtctg gcaacagacg atctcccagc tccaccagta cctggatcac
    61801 ttgaaaaagc cgacgtagtt ttcagggaaa tcgagcagcg aacccctcac ccgcaggctt
    61861 tgtttgtcgt cgctctccac aggtcccgga aattcccgtg ccggttcccc ggcgggggtc
    61921 tttcccatgg tttctttgtc cttcggccaa ttgcagaaag gagaaaggtc atgagctaca
    61981 ccaacgtact cgtggaaaag aaggatcgca tcggattcat caccctcaat cggccccaag
    62041 agatgaacac gttcaacatc cccttcgccc gagaactgaa cgatgccctc tgggagatgg
    62101 aaaacgaccc cgaagtcagg gtcgtagtga tcaacgcggc cgggaaacac ttttccaccg
    62161 ggatctctct ggctgagttc aagggcaaga gccacaagga ataccgggaa ttcattcggc
    62221 tgatggatga acacaaccac accatcgcca agatgaaaaa gccggtgatt gcctcggtga
    62281 agggtctttg cctggccaac ggtgcagggt tgtctttcgc ctgcgacctg acggtggcca
    62341 cggaggattc caagttcgga acgaccgcca tcaacgtagg cctcatctgc ttgggaccgg
    62401 cggttcccct ggcccgttgc gtggggcgaa agaagactct cgagatggtg ctcacgggca
    62461 aaatgatcac ggcggctgaa gcggaacgat tgggcctggt gaaccgggtg gtgcccaagg
    62521 agcagctcga ggaggcgacc ctggaactgg cgacgcagct ggcagccaag agtcccctgg
    62581 cgcttcaggt cggcaagacg ggcatctacg ccatgcagga cgttccctac aaccaggccc
    62641 tggaccacct gagccttctc tttgcctccc attgcagcac cgaagacgcg gaagaagggg
    62701 tccgagcgtt cctcgagaaa cgagagcccg tatggcggga gcgctaaccg acagggattg
    62761 ggtcgaaccg tcctatgccg agggggtacg atggaggggc ctgtccggaa aagggccggc
    62821 cgtcctccca agacccctcc cgtcgagggg aggaggaaag ctcctgctct cccggcggga
    62881 gaaagttggg gggaggggga gtccttatcc gcggacaagc cctaaggatg gaaaggtgcg
    62941 ggaaatttcc cttgggtaaa cccccggctt tgccggggga ctcccagagt ttgacagttc
    63001 cgggagtctg ttttgccaat aggcgctgta gcaccaacca agcctgaaca cagtattccg
    63061 gtcacgctgt tacggctgcc ttgaatgatg atgaaatgaa gtacagctga gctggtgagc
    63121 gcgtagcagc acgagtttgc atagtgcttc aagccgcttt gagcggcccg cggtctttac
    63181 aggcctccgg ctttgcccga ggtacctgac tcctcacgaa cccggcatgg tgatccttct
    63241 gcaaagcgag gtgaaacgaa atgaacgttg aagaggtgat ggaaaaggcg gggtttcggc
    63301 tgtcggcaag ttgcgcgggg caagcgtcct acacgaagtt tgtccaacac aagggaaaac
    63361 gggcttacgt cacggtcacg gacgggtcgg gggaaggctt tccccggacc ctggacgagc
    63421 cggtccaggt ggggactttc gacttgagat ccggagacga attgaccccc gtccagggtt
    63481 tttcgtccct gcgcgagtac ctggaatcca tccgggaatg atccgcaggg gcgccgccgc
    63541 cgagcgaagc ttcctccgat tccagatgag gccgggagaa ctttcgcgac ttttccctag
    63601 gaggcggggc ggctttccct tataaaaggg ggggg
//
LOCUS       DAOOVC010000007        62128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_269_length_62128_cov_6.032607, whole genome shotgun sequence.
ACCESSION   DAOOVC010000007 DAOOVC010000000
VERSION     DAOOVC010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 62128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62128
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_62128_cov_6.032607"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLM79_01765"
     CDS             <1..898
                     /locus_tag="PLM79_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HOV85056.1"
                     /translation="LARAGTDIHFVARGKHRQAILEEGLQVVSSQGSFRVMVHATSEP
                     DEIGPVDLLLFCVKSFDTVDAARMIAPMVAEDTVLLSLQNGIDNIDKLIGLYGQERVV
                     GATAFVEAAKASPGVVAQRGKVGRIVFGELTGQRTARIQKVLEVFRLAGIDAEISDNI
                     QEVLWSKFLFICGVHGVSTLSRSSLGLVLALAETRELMTGVMREVEVLGRKKGVSLPE
                     TVVQDAIALAESYDKRFKCSMLQDLEWRRPLEIEALNGMVVRMGKSLGITTPFNLAIY
                     GCLKLRNQKILDPFWADQLECF"
     gene            complement(1021..3909)
                     /locus_tag="PLM79_01770"
     CDS             complement(1021..3909)
                     /locus_tag="PLM79_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOV85057.1"
                     /translation="MAAVNSPKESPINASASTPSEHSLSPEPDPLSPGNRSLATCRVF
                     ASIAGLLALAAMAGWIFRVPPAHESLVPMAPGTALGLCLLSGVLLLRSWGSLRRGRLW
                     VCRVGAVLTCLLGGLVLAASLGSSDWRADFLILHCTVDVPPMPIGATALSGSCLFLLA
                     GGSLLVHLYRPDSKDTSRPIAAGLALGVLSVALMLLFGYADSKPVSFHIETMPGVLPT
                     AAAFALIGAALLSLSVTPLKPLHKNLPVVAVLVLGTALSHYSFKHTLCLETERIQKRF
                     ERISANLVTSLKRGIENDLEVLWSLAGLFDAFGGVDRRGFGIYMERFWGKHSFYAVVW
                     APVVEEAAGSRYVEEVRRSGIPSFLITELDGDGAIVPARSRAEHFPILYFEGTEGRER
                     ALGFDLASIPAQNDCLRKARTTGRMTAVHGFGLLDETGKFSSLSVALPVYASPSSGHP
                     FEDSSPPPTGFIVGFLRAQDLVQEAVRDLQRAGVEVSVFDITSGEKHLLHTTAENLSD
                     SSAPLSRTFEIPFADRTWMIRTTLSREAMVVPGLWHYWWVPATGVLATVLLATYFFAS
                     SLHRSQLREILGELRETQVRFDQLTRTLKGCFWLKDAKTGNPIYISPGYETVFSRSRV
                     LRYRNPRDTLEIVHPEDRSLVAEALDAQLRGEEVEVRYRILLDNGSIRWIWGRAFPVV
                     NEKGVFHRIAGIQEDITRQKEMEQQVINAKKLEATTALMGGIAHDFNNLLGIILGNIN
                     LAQLDLPPDCAASRLLNQAVRAARKAGELTRTFITVFRGGDPQSSSVSVPDLLEESMA
                     VVLRGGKVQCEASYPEDLWNIEADAGQIRQVLVNLILNAKEAMPHGGIVGVWAENLGK
                     HDIPDHSRAFLHRPRYVKISIQDHGIGIPRQDLEKIFDPYFSTKDRYSDKGLGLGLTI
                     VQSIVKRHGGHIDVESKEGIGTIFYLYLPAAEEPEKTGPPSGPEATGI"
     gene            4360..4908
                     /locus_tag="PLM79_01775"
     CDS             4360..4908
                     /locus_tag="PLM79_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOV85058.1"
                     /translation="MEFRFCPYCACSLTHREDGSVKRLHCSKCDHTFYRNPTAGVAVI
                     LLERDELLLVRRQGPYEGMWCIPCGHVEWDEELRAAARREMIEETGLDVSIGPVFAVH
                     SNFHDPLRQTVGIWFLGRRIGGELRAGSDAKEVRFFPVDGLPSEMAFPTDRVVCGKLE
                     RCLESGDLLLLQDSCLIGEFAV"
     gene            complement(4905..5669)
                     /locus_tag="PLM79_01780"
     CDS             complement(4905..5669)
                     /locus_tag="PLM79_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOV85059.1"
                     /translation="MGDIKIQDNSNGRSADFFRGLQVVAFDCDGVLFDSREANVRFYN
                     HIMETIGHGSVLPEQREYIHMHPVKESLRFLLGNGPLYDRAWAYSQEIDFKDFNAQLR
                     VEPCLVEVLRAAKAGYRTALATNRTISTHAVLAYFHLDQYFDLVVTASDVQHPKPHPE
                     AMERIQQAFGVTPDRILYVGDSPVDELFAASSGVYFAAYKNPRLKAHLHLTHFRELHR
                     RLLGSESPGPGELPPPCEIGGEMSPDGDPARRTFRF"
     gene            5835..7508
                     /gene="nadB"
                     /locus_tag="PLM79_01785"
     CDS             5835..7508
                     /gene="nadB"
                     /locus_tag="PLM79_01785"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOV85060.1"
                     /translation="MEYHYDFLVIGSGIAGLSFALKAADRGSVAVVTKKDKLETSTNY
                     AQGGIASVLGQDDSFQLHIQDTLNAGGGLCHPDVVEMVVKTGPDRVRELATRGVDFTS
                     TGSEITHFDLGREGGHSRNRIVHARDMTGRAVEKVLVAAAEEHPNIHIHENHLALELI
                     MDHQSVKAGSLVLYHRDICRGAYVLDSNTQEIHTFLAHAVMLCTGGAGKVYLYTSNPD
                     VATGDGVAIAYRAGAQIANMEFVQFHPTCLYHPHAKNFLISEAVRGEGARLVDKRGRA
                     FMENYHPLKDLAFRDVVARAIDTEMKKTGDDCVFLDITHRDSAFLKQRFPNIHEKCLS
                     LGIDICKDPIPVVPAAHYMCGGIVTDLWGQTSLENLYAIGECACTGLHGANRLASNSL
                     LEAMVYAHQAAIHSTSQLDEWRVRKFPKVPASLGRQGSQDNLQSEMVLIAHNWDIIRR
                     LMWNYVGIVRTDKRLALAHNHMAQIWMEIREHMPNIPVNSDLIELQNLAMVAELIIRC
                     AIRRKESRGLHYNLDHPQTDDVNWRKDTLVSRTLTDDGVCVVCDRKAHP"
     gene            complement(7626..8123)
                     /locus_tag="PLM79_01790"
     CDS             complement(7626..8123)
                     /locus_tag="PLM79_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85061.1"
                     /translation="MDIGRFFRGPRKKPPKEETREPSINQGIFYLYLIIFVQVVFVVG
                     LAVLIMVIGQVIITPLWVFLFGFLMIAGGLLYIYRKAKKQFRKLRETLQNVDLSGRNY
                     EISIMGGVLTMRVEQAVAAPLLEAPKRPALEPPSGEGTTGNSADPPASGPVPRTSEAR
                     DLGTG"
     gene            complement(8140..8940)
                     /locus_tag="PLM79_01795"
     CDS             complement(8140..8940)
                     /locus_tag="PLM79_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85062.1"
                     /translation="MKGKLRLLDIFEGKRQLKVALAVSIFSFLLLELILILAALNQTG
                     KQSRILITDSQGKVVADRAGSTLTAYERLEFEKTFGPLKDYQVHLQSETVPFPFRAWA
                     SAAVGVPIGLVLLIAFVIRAYLALVYGEEDSGGGDSRLSPSPKGRWGSLFDFSRRVTV
                     FHVGFLVAAAVILLWVVPNFLGDLAKIGAVAIEKYKGFFVACAVFLASLITWIVYLRY
                     RLSKQLLDNQLDLEKYRVEKQLLLQNQPPSPLLPEPVQKNATLEESHP"
     gene            complement(9245..10495)
                     /gene="nrfD"
                     /locus_tag="PLM79_01800"
     CDS             complement(9245..10495)
                     /gene="nrfD"
                     /locus_tag="PLM79_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOV85063.1"
                     /translation="MDKALIPEGVKRCPLPVFALWLLVLFGIIGWGVYAGGVVLLKAL
                     NVTGLNDYFGFGFYITIDLAIIALGAGAFFSGFLYYGMSRFFPALKELHKIINLAVVI
                     GFVCYTGALAVLLLEIGQPIRGWFGYWHANVHSMLTEVIFCISCYAIVLTIEYIPIIL
                     ENRKLAAVRPFHVFGHSLHEIMAVFALTGTFLSFFHQGSLGGVAGVLFGRPFAYRTGF
                     FIWPWTFFLFVLSAIASGPALTGLVCRTIEKVSGRQLVDRSVYELLAKIVGGFLALYI
                     ILKIADTLYWAMVLAPGFGLQLTDFYREPYGMWLLWTEILVCGVLPAIILLIPQARKN
                     NLLLFGAFILDCIGVTINRFVMTVQAIAVPVMPFDHWEVYVPTMYEWAPGIAMIAYCA
                     LVISLSYRYMPLFPRERELNPVSP"
     gene            complement(10594..11346)
                     /locus_tag="PLM79_01805"
     CDS             complement(10594..11346)
                     /locus_tag="PLM79_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV85064.1"
                     /translation="MSEHGHTQSGARYGMVIDLDKCTGCMACSVACQAENNISFRADE
                     SNKVRSITWMEVYSLQNGKDYPEHQVTYLPRPCMHCDAPHHSPCTFVCPVNATTRDEL
                     TGVVSHVYPRCIGCRYCMVACPYHARKFNWWDPTWPKSMEAMLSPEVSTRMRGVVEKC
                     TFCNHRLNKARSKAILDGADLKTVTYTPACAEACPTKAIAFGNLQDPESEVAKLAKDP
                     RAFRILAKLHTEPKVIYLSKHQWVHRLADNSL"
     gene            complement(11350..13752)
                     /locus_tag="PLM79_01810"
     CDS             complement(11350..13752)
                     /locus_tag="PLM79_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV85065.1"
                     /translation="MKFGRRAFLQFAAGAVGGSLLTPIPWKLMDDSAIWSQNWSWRPS
                     PERGPITRKSSVCVLCEGGCGIQARLVNGTRVIYVEGNPKHPVNQGGICPLGAAGAQF
                     LYAPYRVSQPMKQVSKRGNAKGFQPISWKDALAELGGRLSKLRSEGKAAAAACIASAR
                     CSSMSLLWERFFDSYGSPNLFRMPSHGNSLQLAVQLTTGQKRDVGFALERASYVLSFG
                     SNLIEGWGSPGRMQTAFALWQQDLPERGRTRVVQVESRCSMTASKADRWVAVAPGSEP
                     ALALAVAHVMVKENLYDKDFIAASTFGFEDWTDAAGKTRQGFKNLLLSAYAPEQVAEQ
                     VGLDAGKIRELAREFAGQKNAVAVWGSGAGDCAENIYSDLVFLALNVLKGNLRTGGLV
                     SFVPRVPFEPLPEISSGAPGPPPLRLDLAKSPKVPLPGNGLHAFLDAAATGGPYPIDT
                     LMVHEANPAYELQENGLFYAALEKIGFLVSFSSYLDETSVQADLILPNHSAFERYDDV
                     IGMVGAPYAYYAVAAPVLKPRLDTRHTGDVVIELAKDLAGGMEAALPWKSYEDFLQGR
                     VKGLAASGKGVVIDGKEVVPWKLEEGQPLTPNFKDGTDLWKKLVGGLCWVNAPVNSMQ
                     EIETDSGDYELACQSLLKKGFVGEDRKYLPHLALEPPRGSEKDFPLYLVAHPASNLAG
                     GFLPNPPFLNKTLFDTQLLGNDMFVQIHPQTARSLGFKQGDRAVLETPKGKASVRVHL
                     SAAARAGVVYIARGLGHEAYDEYIRDKGVNSNQVVEVQLDPVTNLGTVWANRAQLHHQ
                     RG"
     gene            complement(13834..14391)
                     /locus_tag="PLM79_01815"
     CDS             complement(13834..14391)
                     /locus_tag="PLM79_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOV85066.1"
                     /translation="MKEEQKHSSGSSVLGLVIFFSGFIVALAVGWIVFPSLLFSERIQ
                     PINFSHAAHQDSTCEDCHAFRPDGTYTGIPQLDKCKECHESQLGQSESERILVEEYIA
                     KDREIPWLVYAYQPDNVFFSHAAHKAKNVPCVRCHRDVAKEQKLPAYRENRLTGYSEG
                     TMKMIECEECHAAQSANNDCGICHK"
     gene            14976..15773
                     /locus_tag="PLM79_01820"
     CDS             14976..15773
                     /locus_tag="PLM79_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOV85067.1"
                     /translation="MSPRTLPACFRVMTFNLRFENDRDGDHAWSLRKDFVVEVVKACK
                     PAILGTQEGTPGQLDFLREHLPDYTMAAPDRPFDPTCQYPTLFYETGRFHRADSGEFW
                     LSHTPPVHRSKSWDSAFPRMMGYGLLEDREVGKTFYVSVTHLDHLGAEARVGQARMIA
                     EWIRAHPGPHILMGDFNQPPDSRVHEILTDPRIGLLDTWQVLGKGQGPEGWTHHDFHG
                     VPHKFRMDWILAGRSFDVVRAEIVRDHKDGVYPSDHFPYWADLCWAS"
     gene            15899..16708
                     /locus_tag="PLM79_01825"
     CDS             15899..16708
                     /locus_tag="PLM79_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV85068.1"
                     /translation="MKESIRVALVQPKPYPAFDDPRNLGHALLLLEKCRGESLDMVCF
                     PEYFPFQGERELAAAAKDLNAYLVAGLVESEGGKQYNTATLIDRSGHILGRQRKRNIG
                     LIEREGLGISPGDGIFRAFTTDFGKIGVPVCIDFWGQPEAGKQLADQGVEVVFNISIF
                     PILRGHWKVGASVRAFDNFFSVVGVNTADYNALIHGKRIHHHGGKSFVLQPPKLLDKD
                     DFRRWLRSLDSTENWISLELNELEQVAVASVHLSTTRRFRREFWNRFGFQR"
     gene            16888..18849
                     /locus_tag="PLM79_01830"
     CDS             16888..18849
                     /locus_tag="PLM79_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOV85069.1"
                     /translation="MKELKRNIYLCMKTLDEAERIWGDRVSCLRTRLERVPTAQALHR
                     VTGEPLTARRSVPHYHGAAMDGYAVKARATYGAGDVRPMILKVGENAFPVDTGDPLPG
                     DTDAVIMIEHVQPVKQDEIEIRAAAFPWQHVRKVGEDIVAGELILPLNHLLRPVDLGA
                     LLAAGVLEVPVYAKPKVWIQPTGTELVAAEEAAESAPGQIVEFNGVVLSGLVRECGGE
                     PLLQPIIDDRYPVLRERMEAALASEADVVLFNAGSSAGTEDYTAAILGEVGEVLVHGV
                     TMMPGKPTLLGLARGKPVVGIPGYPVSALLAFEQFVRPLLFSLQGVSAPAFPETEAIL
                     GRKLPSRLGLEEFVRVILGRVAGKLVAMPLQRGAGVITSLTRADGILRIPQELEGMEV
                     AAPTKVRLLRSREQLDRTLIMIGSHDNTLDVLANELKKRDSRVHLSSSNVGSLGGLLA
                     VRQGHCHLAGSHLLDTVTGEYNFSHIERYLKGTPVRLVRLAARTQGFLLKPGNPKGIR
                     GVRDLVRPDVTFLNRQAGSGTRILLDYLLQKEDLASQGIVGYDQEEFTHMAVAVNVLS
                     GRADTGMAIFAAARALDLDFVPVTEEQYDLVIPESEWEDPKVRMLLDIVVSGPFRRTV
                     AAMGGYDTARSGELVGRWDGREWLLRERT"
     gene            18924..19607
                     /locus_tag="PLM79_01835"
     CDS             18924..19607
                     /locus_tag="PLM79_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOV85070.1"
                     /translation="MFGRKWRRNVALGLVLPALLLVRSPVGSQSGETADRPSEVALLP
                     RANGIKNLIRVPLVRQSKNYTCGVAPLESVLGYSRQSIPEGKLAERLKTTEAGGTHYR
                     NMVRFARNHGYMVRKVLDMALEDLEQWIDRRRPVIVVIQAWADPPVDYPHDWNDGHYV
                     VAVGYDDENFYFMDPSTLGHYAFISRVEFLERWHDKDDTTGEVLNRFGLVLEKDGNLY
                     DPEIVTRLK"
     gene            19611..20609
                     /locus_tag="PLM79_01840"
     CDS             19611..20609
                     /locus_tag="PLM79_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV85071.1"
                     /translation="MARGSASYVRLYENGILQERARILRRMMDRCTLCPRRCRVNRNE
                     GRTGACGVANGPTVAAVHLHPWEEPPLSGTRGSGTIFFSGCTLRCVFCQNYPISQLGV
                     GRILSVEELGRKMLELQGKGAHNLNLVTATQQMPYVVEALLHAVPRGLSLPLVHNTGG
                     YERVEVLRLLDGIVDIYLPDIKYDDPRAAAFCSGREDYVRVNRHALLEMWRQVGGLHL
                     DSKGLAFRGMLVRHLVLPGDLSGTRNCLRFLAARLGPGVWVSLMNQYFPAHRALEMPP
                     LDRKVTREEYERAKACLDELGLVHGFVQGGAEKETGTFLPRNPAPRKRGRKREVPG"
     gene            20691..21125
                     /locus_tag="PLM79_01845"
     CDS             20691..21125
                     /locus_tag="PLM79_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85072.1"
                     /translation="MGPTVVLLVDDEAPFVETLTRRLARKGFVILKAFSGLEALDGLS
                     RRRDVDVVLLDVKMPGMDGIETLRAIKRHHPLVEVILLTGHAVLETAIEGMKLGAFDF
                     LLKPCEMEHLVGKILDAKDRRRDRAGKVLQARAVEAADRRGD"
     gene            21380..23038
                     /locus_tag="PLM79_01850"
     CDS             21380..23038
                     /locus_tag="PLM79_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85073.1"
                     /translation="MEEKGYRKLQWKIIATTLGFALVPLFALGLTIYYQFSVSYTAKI
                     MESLKTLAENRRSAIDLFLDERVSQLNSLAYTHHLDQLKDEEYLNRVFTLVQARSKSF
                     VDFGIIDREGNQVAYVGPYQLKGLNYRNEEWFDAAMLRGVYISDVFMGFRKFPHFIIA
                     VMRREGDRSWILRATIETEIFDTMVKAAQVGQKGDAFLINRDKVLQTTPRFGGEVLDR
                     LPYGNFTKFEGTRVEEMEVDGQAALFGMTWIRNKDWLLVVREDPREELSPLFRARHLA
                     IALVLGGVLVIVLGATFVARVITAQLIQADREKAMLDASLIQSSKMAALGKIAAGIAH
                     EVNNPLAVIKEKVGWLRDLLEEEEIATNENYKEFDDAVRKIDYHVDRAKKVTHRLLGF
                     ARRMEPVQEKVDVNRLLDTTIDFLRNEAHYRSIEIQTDFTETLPQITSDSAQLQQVFL
                     NILNNAIDAIGKNGTITVRSRLTNKDKEIAVCIADDGPGIPSDVLGKIFDPFFTTKEV
                     GSGTGLGLSISYSIVEKLGGRIVVASQVGKGTTFTICLPVNQSA"
     gene            23127..23528
                     /locus_tag="PLM79_01855"
     CDS             23127..23528
                     /locus_tag="PLM79_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85074.1"
                     /translation="MENFRVLIVDDEPDFLETIVKRLLKRKVDATGVDSGPKALKLLE
                     KEPFDVVILDVRMPGMDGIETLREMKRKNPLMEVIMLTGHASVEAGMQGMQLGAFDYV
                     MKPAEIDDLLEKVRQAYERKSLHEERLRAEG"
     gene            23594..24526
                     /locus_tag="PLM79_01860"
     CDS             23594..24526
                     /locus_tag="PLM79_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85075.1"
                     /translation="MSNLWRSVYPFLCSHALSFLALGLSVPVWAFGGWSAWVWILPMI
                     AASEIVIRDFQGRIRRANEQKCFLDEQLIQSQKLASIGELSSGIAHEINNPLAIIGQE
                     VEWMGHLLKKESIGDPREMEELKGSMKEISRQVDRCREITHKLLDFARKREPLLQGVD
                     INRLVEDMARLVEREATHHGIRIVREYRKDLPVVYTDAPLIRQVILNLLNNATYAVQK
                     DGTITVRTRRADPEAITVTIEDTGCGISKEHLNKIFDPFFTTKPPGKGTGLGLSLCHG
                     IVTKLGGQISVSSEVGKGSKFTLRLPAQSKKGEA"
     gene            24529..24963
                     /locus_tag="PLM79_01865"
     CDS             24529..24963
                     /locus_tag="PLM79_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85076.1"
                     /translation="MEQASRKPRVLLVDDEERFRTTLGKLLTVHGLSVSTVGSGTDAL
                     EELKKGGYDVILLDVKMPGMDGMTTLAEIKKIDRNLEVIMLTGHASVDVAVEIMRLGG
                     YEYLLKPCPVEEILEKVEAAFERKATRESRLKKGAASPAPTV"
     gene            complement(24941..26359)
                     /locus_tag="PLM79_01870"
     CDS             complement(24941..26359)
                     /locus_tag="PLM79_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85077.1"
                     /translation="MAGKILIVDDEKDMLALLKRIVSEKTSYEVVTTDDPLRVPDLLQ
                     ADAYNVVITDLKMPRMDGIQVLECVKAKDPLTAVVVMTAFGSIESAVEATRKGAFDYI
                     TKPFRKERILHLLDQAVKWQRLQRENSYLRERLSGESGFSSFIGNSPAIQRLHSRIDR
                     VAKTSATVLLTGESGTGKELVARILHTHSLRKDKEFIPIDCSTLPESIIESELFGHLR
                     GSFTGAIRDKKGLVEEANGGTLFLDEIGDLSPPMQVKLLRLLQEGEYKVVGSTSIRKV
                     DIRFIAATNRNLEEKIRKGEFREDLFYRLNVINLRLPALRERTEDIPLLAHHFLEKYG
                     ALHGKEARFSEGVLEYLMERPWPGNVRELENTVERGVIMAQDGIIRLGDLYLSDPGSP
                     APSPRTARPGEEELFSLPLKEAKERLIEQFQSRFIHRVLSKHGGNVSQAARELGVKRQ
                     YLHRLMKDTNIQSKAFKRSEPD"
     gene            26469..28742
                     /locus_tag="PLM79_01875"
     CDS             26469..28742
                     /locus_tag="PLM79_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85078.1"
                     /translation="MKEDSKPEGMATRERVRLRRVIKIVGMITVALVGLALYLGASTA
                     RYMKEIIRSQFNNQQLVFARTSALKIETSIQNAVTDLILLNSSPAIQYCDPEAYEALL
                     LSTLPVLNRDHIQEIRRVDREGNTMFVADEQGVGMKHLGPLRREAGVYLSWASDMSNR
                     GKTMATGLRTQELSKDRKRILIDFVVPTYEDSSDPQHPRPTHRFAGFLAATLNVSSLL
                     QEIMPPVRSGKTGYAWVLDSSGSFLYHPEPSFVGENAFQVRASRNPKISFERINEIQR
                     NEMLHGKEGTGTYFSGWHRELVEPMEKLIAYAPVRLQGPYMSYLWSVAVVAPADEIEG
                     VIHSVYQGQFILQGIIIFIIILGTVIIILYELRWSTILEHEVAVKTRDISRYASELER
                     SEAKYRSLVESAEDLIFMVDQDGILKTANRHMARMFGMEDEEIVGQSLYRLLPREQAN
                     SQLNLVRRVLREGRRVRTETLLCIGEEDFWFNIQYLPVKGEAGGPSFILGIARDITER
                     KGLEKQLINTEKLASLGTLAAGVAHEINNPLGIMLGFCDLLLERMDPGTMEYNDLKTI
                     ERHGLHCKSIVERLLSFARISEETEEFCDLNANVESMVTVVRHTLSLHKIRLETHLDE
                     SLPAVRGDSRGIQQVLLNLINNAVYAMDGQGLLTISTRPSRKRNHAELVVADTGSGIK
                     REFLQKIFDPFFTTKKVGDGTGLGLSVSYGIVSRYGGDIECESYTQEERPGRSGTTFT
                     VTLPFKEGLPPDLSEAS"
     gene            28948..29358
                     /locus_tag="PLM79_01880"
     CDS             28948..29358
                     /locus_tag="PLM79_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85079.1"
                     /translation="MVDPNRMNILIFDPDRDMSELFARALETCRGSKCYLASEEDEVL
                     ALLKEVAFRLLLADLGLIMVNDFSLLRKVRRLFPQVVVVVDTFLHQRDGVSRAMELGA
                     LGYILKPVQIEVFRKKIRDFLSIRHPGTDPDVHQ"
     gene            29574..29996
                     /locus_tag="PLM79_01885"
     CDS             29574..29996
                     /locus_tag="PLM79_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV85080.1"
                     /translation="MAKKVLVPTDGSEHSMKAVRAALELAEQGLADVTLMAVAYYIRE
                     DFEEMPPNVQDKLDAQAKKALRAAKALFEEKGISVETILETGFVPANNIIERAEKGKF
                     DQILMGSTGMTGFFKRLLIGSTASKVVSTAPCTVTVVR"
     gene            30041..30337
                     /locus_tag="PLM79_01890"
     CDS             30041..30337
                     /locus_tag="PLM79_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85081.1"
                     /translation="MIGIRTKWLGILAAVGVFFPAAAMAAAGPAAPLVIVADTRKLSG
                     IMAWWANLYNESHLYFTLLTVVLIPIIGVVFGVVADIVMTHIGIDLKSRDLAEH"
     gene            30389..31462
                     /locus_tag="PLM79_01895"
     CDS             30389..31462
                     /locus_tag="PLM79_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV85082.1"
                     /translation="MDWLYILMPIAGVKIFWPGLIILGIGVGIIGGFFGMGGAWMVTP
                     GLNILGFPMAFAIGTDIAHMAGKSLISTMRHGKFGNVDYRLGLIMLVGTVIGFEVGAQ
                     MVMWLERIGSVEITVRYLYLALLALITWMVFSDVAKRRKKEREARAAGIELEKTATGL
                     EWHKTFHKIKIPPMVHLKAAGIYCSAWLPIGVSFLTGWLAGILGIGGGLIRMPALIYI
                     IGCPTHVAVGTDLFEVMISGLYGAATYTYKGRTELVAAIIMLIGASIGAQIGTVATKY
                     IKGSGIRIAFGLAVVGCAISIVLKLLAMYFPAQKPFYDAAATFFVLGVVAALSIYIAV
                     RMFQGASAEVAARKAAMKKADQG"
     gene            31624..32220
                     /locus_tag="PLM79_01900"
     CDS             31624..32220
                     /locus_tag="PLM79_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4881 domain-containing protein"
                     /protein_id="HOV85083.1"
                     /translation="MGKNRHWVAAWLMFLSLAFMAGCGEYGKVDQGRVIKFDKDKQLV
                     TIIRDVKEDPNNPDYSHLPPITYELPKNPDDVGADPKAGLRMKLDAKNKEIVLFDPVS
                     GGFKTLHYTLLDQKENLSKDDPLIKDKKFPLVDREKKTITIFSGRQKLLVTFSVPDEY
                     FSLPDSAWEAGDEARFYYKEDGKALRYMNVSKTDIFKK"
     gene            32326..33165
                     /locus_tag="PLM79_01905"
     CDS             32326..33165
                     /locus_tag="PLM79_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85084.1"
                     /translation="MDEDQALRWRSSIGKLGALVCAVFLVAVLDGLVARFREPAYEYP
                     ILAGQTLPVNGPLKETPPDAAALAFESSAEGVRLEFEAIQAGFWFGNALWRGTLTVDS
                     GVPPGSYRVKVVAKAIPGTVLSPVIQVVVYADPHDFRFHSKSLFVRYLDISPWWTAIL
                     SLPLVCGSLCAILLLSRRIDGLLAREGKAEIYRVLGAPPEFEVAFGLGTVHGITEGAE
                     LALLDRQGIHVGTVKVREASPVDAVGVAHSMVEVKPGFLVCASSGIRKGSVHDSGLQG
                     LIR"
     gene            33134..34921
                     /locus_tag="PLM79_01910"
     CDS             33134..34921
                     /locus_tag="PLM79_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85085.1"
                     /translation="MIPDSKDSSVKLDSTGRVPKGVYSTLYLRFVALTLLCSIVPLIL
                     VGWGIYRYYSRFGYSRMVEDFQTQVESHRKIVELFLKERVSTLRILSQSHSVEHLSRK
                     ENLDAIFEISQREGSYFTDLGVLNDRGRHLSYVGPYDLMGNDYSGTFWFKEVMEKGVY
                     ISDMFLGFRKVPHFIIVVARTEGDRKWLLRATIYNEFLSALFGNVRIGQTGEVFLLNR
                     EGIYQTTPRLGGLLMGQASLPMDLFREESGVQVLGGSALGEEDASPRLVVAYSWMKEP
                     PWMLVVRQDYAEAFRDVNHANLASLLFLFLSALAILVVSLITTGHMIKVIRKRDLEAD
                     QLNKQLYQACKLASVGELASGVAHEINNPLAIILTEAQVVRDLEEDTPNLDPEFKSQL
                     DESLDQVETQVQRCNLITQNLLRFSRRTKSLIEMVDLNKFLQEVVDLMHKKAQTSGIR
                     FSRNFEEGLPPLQSDPSQLQQVFLNLINNAIDAHDGKPYGTVRISTRTDEKGKGVEIR
                     VADTGSGIARENLDKIFDPFFTTKPVGKGTGLGLSISYGIIQRLGGHISVESELGKGT
                     EFILFLPFRLPESVREGLKGAGTGGGANE"
     gene            34914..35348
                     /locus_tag="PLM79_01915"
     CDS             34914..35348
                     /locus_tag="PLM79_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85086.1"
                     /translation="MSKAKILLVDDEVTFANNMSRLLAKRGYEVSVVHGGEDALRKVE
                     NEEYDVVILDLKMPGMDGLDTLKVLKKRKPFIEVIILTGHGSVDSAVDGMHLGAFDYV
                     TKPVYVEDLNEKIRQAHQRKMMQESKGADGTDVRSHTRFVDR"
     gene            35314..35727
                     /locus_tag="PLM79_01920"
     CDS             35314..35727
                     /locus_tag="PLM79_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85087.1"
                     /translation="MSDPIRVLLIDDEEIFVQSLTKVLKRRGMVVESAGNGALGLECL
                     SQREYDVIVLDLRMPGMDGVETLKAIRDRDTLTPVLILSGHIELGQVSQALREGAEEI
                     LLKPCPVDTLTSAIENAYERKVCARDVERTCGEPD"
     gene            complement(35941..38664)
                     /locus_tag="PLM79_01925"
     CDS             complement(35941..38664)
                     /locus_tag="PLM79_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV85088.1"
                     /translation="MDPTSNPHPPETSASREDGPAAVGRGKRFWKSFLTFLEIIGLRS
                     EDHRIDHELQLARLRLYHTEFRKLLSANNSLLETMGDLERKLISREYIDRAFIKRKVV
                     RILADTHALVESINVISGNRYEALRGAQERISQRLTRAMEEETDSAPGALVMDLQDIN
                     GSQGDWVGGKMANFGELANVVGLPTPEGFVATTEAYRLLVEQGGIRSWIQDKHMEIES
                     QEDVTKVSGELRERLMGLEIPAVLQDSIHSAYDRLCAKTGRQVFLAVRSSAVGEDSDF
                     SFAGQFLSLLNIPKDELCQAYHRVVASLYSPEAVSYRLLHGIPGESAQMGVGFVEMVE
                     AVAGGVVFSRDPGRPELDQVLIQAVRGLGAMLVDGKTSPEVLRVPRGKHGTGILRTPS
                     NQDRQLLMVPGKGVREEDLSPSLAREPILTDEEAALLASWAVKLEDHFGCTQDVEWAM
                     GPQRRIFVLQSRPLLLAAQPVRGVEPLPGFPVLLRGGEVGCPGTAAGPAVHMTDDEDL
                     DAFPEGGILVARRSSPRFVRLMTRARAIVTDAGSTTGHMASLARECRIPTLLNTGKAF
                     QTIPRGCLITVDASSGIVYQGEVPDLLKTEADEAWEEEVSSHRQHTPGYRQLKKVVDL
                     VAPLNLTDPSSSTFTADHCQTPHDIARYVHEKSYQEMFQLGDNVGDLRGASFQLDVFL
                     PVDLYIIDLGGGLKSPAKGGKVKPSQVASAPFSAILKGMFHKKIPRFGPRTMDLGGLL
                     SVMMRHATTSPEQDSSFRDPCYALISDNYVNYTARVGYHFSVVDTYCGNTTNKNYITL
                     VFKGGAADYVRRVRRIRAIADILKEYGFSVRITHDMVNARLSKAPREEILQHLEKLGS
                     LLQFFRQMDAAMTSDDSVRVFVKAFLRGDYGLEYVGEEEPIPGQTNGGGNT"
     gene            complement(38675..39046)
                     /locus_tag="PLM79_01930"
     CDS             complement(38675..39046)
                     /locus_tag="PLM79_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85089.1"
                     /translation="MKVLLVDDEVDFVNSLCKVLTRRGFEVHSATTAYAALPLIAKES
                     FDVVVLDVKMPGMDGIQALAEIKRIEPGTQVILLTGHYSPMDEEDTMKSGAFCYLLKP
                     CTITKLVDAIKAAAEGRCPGP"
     gene            complement(39043..39738)
                     /locus_tag="PLM79_01935"
     CDS             complement(39043..39738)
                     /locus_tag="PLM79_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85090.1"
                     /translation="MLFSHKRVERVVMPPEPEDLRKEIQELYAKRKRGDLREKGFQKL
                     LTFFTIELYRAVIRKRMAKDEPIEREHHSIQAHFKWTQSLLKEPEQEACSVFATDRRL
                     FFVQSKIVPDQPPTADSRDHTRVILLPYDRILGMTVRREVRWGELGVGAVILLSSLIF
                     WEYLDVTSWVMMGIGILGILHAVLLPTRWIELDSSNPSPNPVPVQIHAVGKKSGRQLL
                     RFLREKIREKTKT"
     gene            complement(40191..40385)
                     /locus_tag="PLM79_01940"
     CDS             complement(40191..40385)
                     /locus_tag="PLM79_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85091.1"
                     /translation="MQILANIVLYGLLLWQVYESTIAEWDPWNAFVVVFVLLVAVTLT
                     LLPGYHASHVSPGSYPYEDD"
     gene            complement(40559..41626)
                     /locus_tag="PLM79_01945"
     CDS             complement(40559..41626)
                     /locus_tag="PLM79_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV85092.1"
                     /translation="MFDFAIAGIQSPVFLLVLTGLTVGIVGGFIGVGGGYMVTPALIV
                     FGFPGYMASGIDVTHIAGKSVVATIRHRQLGNIDWTLGLAMVGGTMMGVEWGVRLLNM
                     TKKAGVSGIALLSATVVIMLSLFIYTQIETRKAHKQIEEREKSGAHLGREHKATRLPE
                     YFQKIPLFPIVRCHVARIVISMWVIVVVGICTGLLAGFFGVGGGFIRVPALVYIIGAT
                     THIAVGTDLLEIVVSGGYGALRQSMSGNVDMMAVLFMISGAMIGAQFGALATSFVRGP
                     AIRYILSYSLILATVGAFMRLGYLMTGGQYEILSFAAVVFTLGEMIFLCLFIMSLVIF
                     AARHKAGKPVPNWIPSLVVKD"
     gene            complement(41640..42197)
                     /locus_tag="PLM79_01950"
     CDS             complement(41640..42197)
                     /locus_tag="PLM79_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85093.1"
                     /translation="MDDYRRRETIKFIVWTVLSLALVFYICYEYYSGEMISWYYYETS
                     TTGYAVDVNTFKDATKDKPMKLEIVQAAEIKGLQAVPVKKGDRLPKNANGVISKKDVE
                     EGKRVALEGNTLKVMVPSQIKEAKGFKYRDTYKHKGVQTNPWSGVWNVAVILALGFCL
                     GTMAEGFTGMLGMKVKKIKHYEGGH"
     gene            complement(43176..45785)
                     /locus_tag="PLM79_01955"
     CDS             complement(43176..45785)
                     /locus_tag="PLM79_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV85094.1"
                     /translation="MLEKLNPFRWVGRKHTRPREDLSDIFRLKYQHFKELLDANTQLS
                     KIITDIEQKLRGEQLFGMSYVRSQSARAVFCTLRMIQGLNGLSSNRYLLLHEKLDSLN
                     RLIKDQLEKRKENPVQELVLPYSKITREMVDWVGGKNSNLGEVLNRAHLPIPEGFAIT
                     TRAYELLLRANDLVDEINKIKMLTDPRNPESVNEASEEIQRLIISASVPEELKEAVLE
                     AYDRMMERIESRTTGGFCRDVSLRSSAIGEDSELSYAGQYLSVLNVPRSRLIQTYNYI
                     VASLFTPRAIAYRFNLGIRDEDIAMSVACLQMVDSVCSGVMYSHHPFNRFQDHVLISA
                     VWGLGPYAVDGVITPDRYTVAKDGHYTLLESDISHKPVQLVRNPEGGLMQVPVPDDRQ
                     QAPCLTPEQVKTLADYAVKLEAHYGIAQDIEWAVDPEGRLLILQTRPLRLEAPSQGGE
                     TVQVPEPAGYPLLLEGGQAACPGVGFGPAFHVVSEEDLGNFPDGAILVAANSSPKFVV
                     VMPKAQAILTDTGSITGHMASLAREFGVPTLLGTRSATRRIPHGTEITVDAFYGKVYE
                     GRVPELLQLRKDREPRMKRTPVYETLRSVAQWIVPLHLFDPKAPEFSPLHCESLHDIM
                     RYVHELSYKEMFQISDVVSERGQCGVLLQAPIPLDLCVIDLGGGLAPRKPHPRKVSVE
                     EVVSVPFKTLLRGMLHEGVRYQRPRPVELGGFLSVMSEQMLSTPHTADRFGDRSYAIV
                     SDRYLNFSSRVGYHYGILDAFCDEVISKNYITFSFKGGAADGVRRNRRARSIALILET
                     IGFAVEVMGDRVDARYQKYDLGLTQEKLDLLGRLLLFTRQLDMLMHSEKSVREIARAF
                     LEEDYQLERAFCA"
     gene            46267..46809
                     /locus_tag="PLM79_01960"
     CDS             46267..46809
                     /locus_tag="PLM79_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85095.1"
                     /translation="MVSDARSGVAVILVLLLLSSCAEFTFAPLAKHKAPPAGDFRLLG
                     VQVPESVQEDIPYDITGTFQATGEVKVLRACFRWFSDRAQMSSPPLHCFTYEVQTNQP
                     IGSVCSRWVAEGPHAIMSPLTCVRVEDVRYGTPGRFAVKLRSSNLKLVYSQIECYLEY
                     LQDGVTKESNKITARIRVGE"
     gene            complement(47098..47718)
                     /locus_tag="PLM79_01965"
     CDS             complement(47098..47718)
                     /locus_tag="PLM79_01965"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV85096.1"
                     /translation="MTQTKEKEKDKDISLAVRALKIGNKVRELRQKNRFTLQDLAART
                     GLSKPFLSQIENDHVVPPVATLLKLARALNAGMAYFFQDEVGNEKVSITRQGERIRVE
                     RRPHQSQGEVNYIYEALETKKTNKNIEPFLIEFPPQQTDEMIFMSHDGEEFMYIMEGT
                     VEFRTVDRVEILGAGDSIYFESDLSHSFRCISDKPAKALAAVWSRP"
     gene            complement(48282..48851)
                     /locus_tag="PLM79_01970"
     CDS             complement(48282..48851)
                     /locus_tag="PLM79_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV85097.1"
                     /translation="MRGIRERRSIRSFQDRDVPDDLLSQVFEAVRWSPSRANPRCREI
                     IAVRDPGLEKRLRETVGPGNAAAKSVVSAPVVLVVCGKLGRAGHYHGRVTTRFGDRLL
                     FDLGIATQNLCPAARDPGLGTVIVGLFDHEEAGRIFEIPPEHQPVALVPPGCPSRVPP
                     APRRREAAEFTHFKRFRNDLKPGKPPGLE"
     gene            complement(49356..50114)
                     /locus_tag="PLM79_01975"
     CDS             complement(49356..50114)
                     /locus_tag="PLM79_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOV85098.1"
                     /translation="MHDLYFHHPRWPLLYEDNHLLALYKPAGLLVQADQTGDVCLLDL
                     GKSWIKERYRKPGAVFLGMVHRLDRPVAGVMVFARTSKAASRLSAQFRARRVEKFYLA
                     VLEGTPPRDRETLVHRVERRGDRSSRVAPQAGEGQEARLSYRLLCSSGPVSLVEVRPE
                     TGRRHQIRVQFSHLGCPVLGDLRYGARDPLPGKQIALLAWRLLVEHPTRREPLLFESP
                     IPEGWPLPPQGSPPAGTPWTWKDLEKLLEPGPPE"
     gene            50303..51721
                     /locus_tag="PLM79_01980"
     CDS             50303..51721
                     /locus_tag="PLM79_01980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV85099.1"
                     /translation="MGALLRESGIRLPPRGLDQLWSYHQLLRQYNPDLNLSRISNFTN
                     MVQKLYVDSILPGQILELPSPLLDLGTGPGMPGIPLKIAFPRLEILLAESRQGRAAFL
                     ERVLQELKLSGIEVVPMGIGPRYERPVKGVITRAVESVEETLRRVRGCLDQHGLVIFM
                     KGPGCDAEVDTAVARFSREYRLVTDHAYGIAGTPHKRRLVVFQRLDEPERVRKVLAME
                     HRVVRTIESEGNREFKSLRKLLGSRGVKKEQRALVSGPRQVAEVIESHPQACDAWVSE
                     LEGPAPPSGVPRPVSWVRLSPELFRILDLFGTGSPLLVVKIPPIPPWEPSRGLPGGCS
                     LLLPFQDPENVGSAVRSAAALGVSQIILLEECAHPFHPKAVRASGGTVFAVKFLRGPS
                     LETLPDLPGVIPLDARGKNIEEIEFPPDFALLPGIEGPGLPGPWRSRSAAIPIRSGID
                     SLNAAAAVAIALYVWSRRKETN"
     gene            complement(51727..52323)
                     /locus_tag="PLM79_01985"
     CDS             complement(51727..52323)
                     /locus_tag="PLM79_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOV85100.1"
                     /translation="MAQKKVLLLSAIALWFLASCAGSLQPGKPDASGKSPGAKDSPQA
                     APPVPPSALVGYLDRQERDLRASFSQKDFATIERAENCLAVTFLSDVFFDVNSTLLKP
                     GTSVEIVQLAQILKRYPETQVKICGHTDSSGSERYNQGLSERRAVAVRQVLVESGIHA
                     ERISVEGLGESMPLVSNATETGRQMNRRVTVFLTPKSP"
     gene            complement(52343..53365)
                     /locus_tag="PLM79_01990"
     CDS             complement(52343..53365)
                     /locus_tag="PLM79_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HOV85101.1"
                     /translation="MNAMVLKSCAPIETAPLERREVPDPVAGPGEIRVRVHTCGTCRT
                     DLHVIEGELPPLDRPIIPGHQIVGTVDQPGPGTSRFRVGDRVGIAWLHHACGQCSFCR
                     SDRENLCESSVFTGYHAPGGYAELVVVPEDFAYPIPSVFSDLEAAPLLCAGIIGYRSL
                     RRSECRPGQTLALYGFGSSAHIVIQIALHWGCTVYVCTRNAGHRELALRLGAAWAGSR
                     AEQMPTPADSAILFAPAGDLVPPALKQLKKGGTLALAGIYMTDVPSLGYEEHLFYEKN
                     LRSVTANTRQDGLDLLEVAARIPIRPRTEVFPLAKANEALLRLKQDRLRGSGVLTILP
                     EARQNR"
     gene            53461..57267
                     /locus_tag="PLM79_01995"
     CDS             53461..57267
                     /locus_tag="PLM79_01995"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOV85102.1"
                     /translation="MLLGRKSTNKEPAAAGLFPLRFLVWGSAFCLFVPLVFLFAIHLP
                     ALQEQLIGKLIREVERATPLQVRLGSYRLRPFSRLELLNLNISASGKELLVCDQASLV
                     YRISTTWPYVHATELLLEKAVLNLERDTQGNWLLPRNRNADKHFPGRVHPDWVRIPLP
                     QVRIVSGTVTAHQEGKPVLTLRNVNGTLSFRVVEEKEGPRLIIEMGGLSQERSAREPQ
                     GGDSAGSKGLKPSNLSYLTIDGSSQVAGRGTVDLFPDGEVDVEVWLDPVSRSLFPALA
                     EQVGELKELTGYVRARRQGGAWTVDHDWNTNLGTARGRATVGWENAAEGTSGTKTVRW
                     SGRFENLAVPPVASPGPGGLFGDIEVEARGRDLSDMEIRVKGHLDPSNWGDQRIQTGI
                     LSGSYAGGVASLEAVEVRSSLGNFSARLWADVRGAWDPHHAGKAGVDLQVERGALDKV
                     LPGAKQRAGGSVKAEGRYSPGRFRDWKDWQARVEGTLNLPDFFSLKASGTYENALLRF
                     DYELEAPEIGKVSALVPSWEGKGRISSRGSFRGTWPDLVWEGSVSSPRLEYGPLVADQ
                     VAVNGKGKVLGRDGHRKAALKARGWSLHGNKMGAVQADLEQQDGSCRFEIKGEQLWQG
                     TSARLAGLLENLWLPTRTLVLKPSRVNWKDQAGSVEGRIEFSGDRIQIHSLTIQQNRQ
                     KIQLTGAFAPDARADLTLNLDSVQVAPWLRFLDKNPVEAGSLGGQIRLTGRVEQPEAT
                     LNLQITNVTLNPEVFRDKSSGEALNRGTGRDTRGQGSQTVEKILVTGNFSRDALQVKA
                     DLQAAAFSAPAVVTARIPLRFSLDPPRLDIAKTDEWRSTLRVTGFSADRILPYVEDLS
                     KVGGRIDGEVEGGGSWSQPFLKGSGTLQDGDFTVKAWPHPVEKLRGDWSLDEKFIHIR
                     SAEGQLLGGRVEVKGRVRFPSFRVAELEATGENLGVTELYGIRGRVSGKARLSELSEN
                     PELTADLRFSKAEMQLGDLETDIARNIQIVGGDEQETLVEVQSRSRKKPNYFHRMKME
                     LHLGLPDSGSWVRGKGLDAEINGSMTIEKGPHGPVRLLGSFYALRGTYNFQGNQLKVV
                     EGELIFRGSPEPDPVLKIICQKQVKDVTVQVQVTGPVSQPKLALSSVPSMNQVDVLSY
                     LLFGHPAGELNTKQQFQLQDRAASWIGSQTSHVLKDVFGNNPWTPDTLHYRSSDDRKE
                     GAVVEIGKYVTPDLYVTYGKGIRGETENQVQVEYRLNRHLSVQTQVGGAEQSGVDVFW
                     RYDFGK"
     gene            57282..57485
                     /locus_tag="PLM79_02000"
     CDS             57282..57485
                     /locus_tag="PLM79_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85103.1"
                     /translation="MKMQDVRVMAKSMGIKCFGKTKANLIREIQRTEGNFDCYGSARE
                     HCDQEACLFRSSCLDENKEKPKK"
     gene            57504..57851
                     /locus_tag="PLM79_02005"
     CDS             57504..57851
                     /locus_tag="PLM79_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85104.1"
                     /translation="MTQSSQTVQENVLLTLQNSRKPEQVVSVTMDPTRKRLQTRGLKD
                     LFGVDEIRFETRDLVENLQEIAQVISFLFETISRAEDLHLPYGYQDEFEFQGIRYTLY
                     KEEGVRVLRHKER"
     gene            58012..58473
                     /locus_tag="PLM79_02010"
     CDS             58012..58473
                     /locus_tag="PLM79_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85105.1"
                     /translation="MKRRFRVLFWLFALAAFAGTLSHWPAREDGGSLSGSVPGSLPDL
                     SPTEVMAQTATPGPSFAEVAAIFKKYHCTVCHGGASPSRGLSLDNREGVLKGGKKGPV
                     VIPGEPGKSELLKRIRGVSEPRMPYDGPPWLTEAEGAVIEGWIASGAPANG"
     gene            complement(58482..59771)
                     /locus_tag="PLM79_02015"
     CDS             complement(58482..59771)
                     /locus_tag="PLM79_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV85106.1"
                     /translation="MQIVATHSMTDFDALASMVAATFLYPEAIGALPTRLLPNVQEFL
                     AIHKDLFRLCSVREIDPSGVTSLVIVDTNHWSRLDPLKDLQDRTDLEISLWDHHMEGG
                     NVHASWSCREPTGAAVTLLLRRMREGECAFTPMHATLFLLGLYEDTGNLTYPSVTPED
                     AYTAGFLLENGADLNVAGAYLASSFDDNQTEVLTQLLNGATLYRESGYHIGTSLLRIH
                     GGGGLLAPVVAKFRDIKHLDVAFAVFATDQQRSIIIGRGGSSDVNVGAVMRRLGGGGH
                     FGAGSAVVHGKDPDVLYRELETLIRAEIRRPRLSVRDIMSRAEPFLSSNSGMEAARTL
                     LAQSRLRAIPVVENGEFRGIVSEVECCKPRTPAQMKAPVRAYMKTRIPTLHPDQNIRE
                     SLRLMTEVDSYLLPVIEDGKMIGMVRRADLILHMYRF"
     gene            59945..61120
                     /locus_tag="PLM79_02020"
     CDS             59945..61120
                     /locus_tag="PLM79_02020"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HOV85107.1"
                     /translation="MQKPGATFLLLLLICLLSSVLTAGAAHGSDVSPPSHRCSGEELD
                     SLLAPVALYPDSLLSQLLMAATYPLDVVAENQWLQQNPGLEGNALDGALEGKDWDMSV
                     KSLAFFPQVLTMMDDKLDWTTRVGDAFVNQEDDVLDAIQRLRSKARAAGNLETNPEQK
                     ITSQESKIRIEPVDSQVVYVPVYNPVVIYGPWWIPAHPPYVVVPNGYVAGPWGIAFTD
                     GYFAGSVLYGGFDWWGGEVVIQDNGFVRKHDKHDRHHEVNRVWRHDADHARAARENAA
                     RRASGKSAGLEAGRADRASGAGDLSRDFRGYDRSSQKSDLIRAGASTEHGSREPGESR
                     AFDAREAGPDARESSARGAQSRWSMGFSESRDFSRGMGGGAFGGGGFSHGGGGGRRR"
     gene            61218..61658
                     /locus_tag="PLM79_02025"
     CDS             61218..61658
                     /locus_tag="PLM79_02025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOV85108.1"
                     /translation="MIYHVEVEAGEAGNRRVECRVRNHVVRADQPREFGADDTAPTPP
                     ELLATALGSCVVSTVQFLAMQRGRDVRNIRVTVEGEIDFSKAMGLGHGGRAGFRDLKI
                     RIGFDSAMTREERADFMKAVFDCGACIDSLQDPTPISWEWRSGE"
     gene            61724..>62128
                     /locus_tag="PLM79_02030"
     CDS             61724..>62128
                     /locus_tag="PLM79_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOV85109.1"
                     /translation="MKLTVSLAQMNVLRAQPRENLAKGEALIAEAARRGSDVVCFPEM
                     WTTGFDWESNARTARDHERIVEQVGRLAEKYGIWVTGSVLALNEEGKVSNACILFGPG
                     GDRVGTYRKTHLFSLFHEDRHMAPGESSCLAET"
BASE COUNT        12524 a        18141 c        19391 g        12072 t
ORIGIN      
        1 cttggcacga gcagggacgg acattcattt cgtggccaga gggaaacatc gtcaagccat
       61 cctggaagag gggctgcaag tggtttccag ccagggcagc ttccgggtga tggttcacgc
      121 cacctcggaa cccgatgaaa ttggaccggt ggaccttctc ctcttttgcg tcaaatcctt
      181 cgacaccgtg gatgcggccc ggatgatcgc tcccatggtg gcggaagata ccgtacttct
      241 gagcctccag aacggcatcg acaatatcga caaactcatc ggactctacg gccaggaacg
      301 ggtggtgggc gcaacggctt ttgtggaggc cgccaaggcg tcgcccggag tggtggccca
      361 aaggggaaag gtcggccgga tcgtttttgg agaacttacg ggacaaagga cggctcgtat
      421 ccaaaaggtc ctcgaggtgt tccgcctggc ggggatcgat gcggaaatat ccgataacat
      481 tcaagaagtt ctctggtcca agttcctgtt catctgcgga gttcacgggg tgagcaccct
      541 ttctcggtcg tccttggggc tggtgttggc ccttgccgaa acccgcgagc tcatgacggg
      601 ggtcatgcgg gaggtggaag tgttgggtcg aaagaaaggc gtatccctgc ccgaaaccgt
      661 ggtgcaggac gccattgccc tggcggaatc ctacgacaag agattcaagt gctccatgct
      721 ccaggatctg gaatggcgaa ggccgctgga gatcgaggcc ctcaacggga tggtagtaag
      781 gatggggaaa agcctgggga tcacaacgcc cttcaatctt gccatctacg ggtgcctcaa
      841 gcttcgcaat cagaagatcc ttgacccttt ctgggcggat cagctcgagt gcttctgaaa
      901 gcaaattcgc ggccggggtg ctccctgtcc gggcggcgct ccctgtcccg gaggagagcg
      961 ggatcgccgg ttttcccggg tcggatgggg gccccgaagg gtcgtttgag gtctggggaa
     1021 tcaaattccc gttgcttcgg gccctgaagg gggaccggtc ttttccggct cctccgccgc
     1081 cgggagatac aagtaaaaga tcgtgccgat tccttccttg gactccacat cgatgtggcc
     1141 tccgtggcgt ttgacaatgg attggacgat ggtgaggccc aatcccaatc ctttatcgga
     1201 gtagcgatcc ttggtggaaa agtaggggtc gaagatcttc tccaggtcct gtctgggaat
     1261 gccgattccg tggtcctgga tggagatctt gacgtagcgg ggccggtgca ggaaggccct
     1321 ggaatggtcg ggtatgtcat gtttgccgag attttccgcc cagacgccga caattccgcc
     1381 gtggggcatg gcttccttgg cattgaggat gagattgacg agaacctggc ggatctgccc
     1441 ggcatccgcc tcgatattcc acaagtcctc cgggtaggaa gcctcgcatt ggaccttccc
     1501 gcccctcagg accacggcca tggattcttc cagcagatcc ggaacggaga cggaggaact
     1561 ctgtgggtct ccaccccgga aaacggtgat gaaggttcgg gtcagctcgc ctgccttccg
     1621 ggctgcccgt acggcttgat tcaaaagccg ggaggcggcg cagtcgggcg gcaaatccaa
     1681 ttgggccagg ttgatgttgc ccaagatgat ccccagcagg ttgttaaaat cgtgagcgat
     1741 ccctcccatg agagccgtgg tggcttcgag tttcttggca ttgatgacct gctgctccat
     1801 ttccttctgg cgagtgatgt cttcctggat cccggcgatc cggtgaaaga cgcccttttc
     1861 attgacaacg ggaaaggccc tgccccagat ccagcgaatg gaaccgttgt cgagaaggat
     1921 ccggtagcga acctccactt cctctccgcg cagttgggcg tcgagggctt cggccaccag
     1981 ggatcggtcc tccggatgga cgatttccag cgtgtccctg ggattccggt aacggagaac
     2041 cctgctcctg gagaaaacgg tctcataacc cgggctgatg tagatcgggt tccccgtctt
     2101 ggcgtccttg agccaaaaac agcctttcag ggtgcgggtg agctgatcga atcgcacctg
     2161 agtttccctc agttccccga gaatctcccg aagctgagag cggtgcagcg aagacgcgaa
     2221 gaagtaggtc gccagaagaa cggtcgccag gaccccggtg gccggaaccc accagtagtg
     2281 ccacaatccg ggaacgacca tcgcttccct ggaaagggtg gtccggatca tccacgtgcg
     2341 gtcggcaaac ggaatctcga aggtccggga caggggtgcc gacgaatcgg aaagattctc
     2401 cgcggtggtg tgaaggagat gtttttcccc ggaagtgatg tcgaagacgg agacctcgac
     2461 cccagcccgc tgaaggtctc ggacggcctc ttgaaccagg tcttgcgctc gaaggaagcc
     2521 cacgatgaat ccagtcgggg ggggcgaaga atcctcgaag ggatgtccgg acgacggcga
     2581 tgcatacacc ggcagcgcca cggaaaggga ggagaatttt ccggtctcgt cgagcaatcc
     2641 gaatccgtgc acggctgtca ttcggccggt cgttctcgct ttccggaggc agtcgttctg
     2701 ggcgggaatg gatgcaaggt cgaaccccag tgcccgttct cttccttccg tgccctcgaa
     2761 gtacagaatc gggaaatgct ccgcccttga ccgggccggc acgatcgcgc cgtccccgtc
     2821 caattcggta atcagaaagg atgggatccc gcttcggcga acttcttcca catatcgcga
     2881 tccggccgct tcctcaacca ccggggccca gacaacggcg tagaaactgt gctttcccca
     2941 aaaacgctcc atgtagatgc cgaatccccg tcgatccacc ccgccgaagg cgtcgaacaa
     3001 gcccgcaagg ctccaaagca cctccaggtc attttcgatt ccgcgtttca gggaagtgac
     3061 caggtttgcg gaaatgcgct cgaagcgttt ctgaatccgc tccgtttcca ggcacaaagt
     3121 gtgtttgaag gagtaatgcg acagggctgt ccccaggacc aagaccgcca cgacggggag
     3181 attcttgtgg aggggtttca gaggcgtgac ggacagggac agcagagctg ccccgatcag
     3241 tgcaaaggcc gccgccgtcg gcaggacccc gggcatcgtc tcgatgtgga aagagaccgg
     3301 ctttgagtcc gcgtatccga aaagcagcat cagagcgacg gagaggacgc ccagggcgag
     3361 ccctgcggcg attggtcggc tcgtgtcctt cgagtccgga cgatacaaat ggaccagcaa
     3421 cgagcccccg gccagcaaga agagacacga gccggaaagg gccgtggccc cgatgggcat
     3481 cggggggaca tccaccgtgc aatggaggat gaggaagtcc gctctccaat ccgaggaccc
     3541 caaggacgcg gcaaggacca ggccccccaa aaggcaggtc aagacggcac caacccggca
     3601 aacccagagt ctcccccgcc ggagactccc ccaggatcgc aagagaagaa ccccgctcag
     3661 gaggcacagc cccaaagcag tgccgggtgc cattgggacc agggattcgt gggccggggg
     3721 aacgcgaaaa atccagccgg ccatggcggc cagggcgaga aggcctgcaa tcgaggcgaa
     3781 gactcggcac gttgccaggg accggttccc cgggctgagg gggtccggtt ccggggagag
     3841 ggaatgctcg gacggagtcg aggccgaggc gtttatcggt gattctttgg gtgagttgac
     3901 ggctgccatg gtttcaggtc accggggcct ttccaaaaga acgcttcccc caaggttccc
     3961 gaggaaatga aaggtcccgg ccatcacgtt tcatctgtca tcttttctat ggatgccgtt
     4021 tcgcgagggg atcggccaca ccccggctgg aagccgattc gttgcttact acagtcaacg
     4081 gaacaattgt acaacattcg gtggccattt caaaagagac aatggtagac tccggattgc
     4141 cgaagggacg gcgtccccaa tacacgggtc gtctgtttcc gcccccaacc gaggatgctc
     4201 tccgcttggg tgttcccccg gaacccgggg gtccggaggg gagtgttttc cggagaaatc
     4261 aaaggggatg gggcgagatt tcccacccga tgccgtgagt tccgggaccc tcttccaagg
     4321 ggcctggaag ctcgaatctt gtcttaggag gaggttgtga tggagttccg tttctgcccg
     4381 tactgcgcct gctctctcac ccatcgcgag gatggttccg tgaaaagact tcattgcagc
     4441 aaatgcgacc acaccttcta caggaatccc acagcggggg tggcggtcat tctgttggaa
     4501 agagacgagc tgttgctggt tcgccgtcag ggtccctatg aaggaatgtg gtgcattccg
     4561 tgcggtcacg tggaatggga tgaggagctt cgagcggcgg cccgccggga aatgatcgag
     4621 gagacggggc tggatgtgag tatcgggccg gtgttcgcgg ttcactccaa cttccacgac
     4681 ccgcttcgac agacggtggg aatctggttc ctgggacgca ggatcggcgg ggaactgcgg
     4741 gcgggttcgg acgccaagga agtacgtttc tttcccgtgg acggactgcc ttcggaaatg
     4801 gcttttccca cggatcgagt agtttgcggc aaactggaac ggtgtctcga gagcggggac
     4861 ctgctgttgt tgcaggattc ctgtcttatc ggggagttcg ccgtctagaa acgaaaggtc
     4921 cggcgtgcgg gatcaccgtc cggcgacatc tcgcccccga tttcgcaggg gggagggagc
     4981 tctcccggtc ccggggactc cgaccccagg agtcgtcggt ggagttctcg gaagtgagtc
     5041 agatgaaggt gtgctttgag gcgagggttc ttgtaggcgg caaaatagac cccggaactc
     5101 gcggcaaaca gctcgtccac gggagaatcg cccacgtaaa ggatccggtc gggagtcact
     5161 ccgaaggctt gttgaatccg ttccatggcc tcggggtggg gtttgggatg ttgaacgtcc
     5221 gaggccgtca cgaccaggtc aaaatactgg tccaaatgga agtaggccag gacggcatgg
     5281 gtggaaatgg tccggttggt agccagggcg gttcgatacc cggcctttgc cgcccggaga
     5341 acctccacca ggcacggctc cacccggagt tgagcgttga aatccttgaa atcgatttcc
     5401 tgggaatagg cccaggcccg gtcgtacaag ggtccgtttc ccagcagaaa acggagggat
     5461 tccttcacgg gatgcatgtg aatgtactct cgttgttcag gcaggaccga gccgtggccg
     5521 atggtttcca taatatggtt gtagaaacga acattggctt ctctggagtc aaacaacaca
     5581 ccgtcgcaat cgaaggcgac gacctgaagt cctcgaaaaa aatcggcgct gcgtccgttt
     5641 gaattgtctt ggatcttgat gtcccccaac ccgctctctc ctttggacga cccccgatcc
     5701 ccagcatttc ccgggagtca tggaaacctt tacatccgtc tacttatcga atagagtgcc
     5761 gggtgtcaag gcggggggag gcgggcgtcc cccctttttc attttcacgg agccaacata
     5821 cggacggtgg cgtcatggaa taccattatg attttctcgt gattggaagc gggatcgcag
     5881 ggttgtcctt cgccctgaaa gcggcagatc gtggaagcgt tgccgtggtg accaagaaag
     5941 acaagctgga gaccagcacc aattacgccc agggcgggat cgcatccgtt ttgggacagg
     6001 acgactcctt ccagctccat atccaggaca ccctcaatgc cgggggcggc ctttgccatc
     6061 ccgacgtggt ggaaatggtg gtgaaaacgg gacccgatcg agtacgggaa ctcgccaccc
     6121 ggggggttga tttcacttcc acagggtcgg agatcaccca tttcgacctg ggaagggaag
     6181 ggggacacag ccggaaccgc atcgttcatg cccgggacat gacgggcagg gcggtggaaa
     6241 aagtcctggt ggcggcggcg gaagagcatc ccaacatcca catccatgag aaccacctgg
     6301 cgttggagct catcatggat caccagtcgg tgaaggcggg atccctggtg ctctaccacc
     6361 gggacatctg ccgcggagcc tacgtcctgg actccaacac ccaggagatc cataccttcc
     6421 ttgcccatgc cgtgatgttg tgcaccgggg gagcggggaa ggtctacctg tacaccagca
     6481 atcccgatgt ggccacgggg gacggagtgg ccatcgccta ccgcgccggg gcccagatcg
     6541 ccaacatgga attcgtgcaa tttcatccca cctgtctcta ccacccccat gccaagaact
     6601 tcctcatttc ggaggccgtg cggggggagg gagccaggtt ggtggacaaa cgcggtcgag
     6661 cgttcatgga gaactatcat cccctcaaag acctggcgtt ccgggacgtg gtggcccggg
     6721 ccatcgatac ggagatgaag aagaccggcg acgattgtgt ctttctggac attacccacc
     6781 gggattccgc attcctcaaa cagcggttcc ccaacatcca cgaaaaatgc ctctccttgg
     6841 gcatcgatat ctgcaaagac ccaatcccgg tggttcccgc ggctcattat atgtgcgggg
     6901 gcatcgtcac ggacctctgg ggtcagacca gcctcgagaa cctctacgcc attggagaat
     6961 gtgcctgtac gggtcttcac ggcgccaatc gactggccag caactccctg ctcgaggcca
     7021 tggtctacgc ccaccaggca gccatccact ccacgtccca gctggacgaa tggagagtgc
     7081 gcaagtttcc gaaggtcccc gcatccctgg gtcgacaggg cagccaggac aacctccaaa
     7141 gcgaaatggt tctcatcgcc cacaattggg acattatccg tcggctcatg tggaactacg
     7201 tgggaatcgt gcgcacggac aaacggctgg ccctggccca taaccatatg gcccagattt
     7261 ggatggaaat ccgggaacat atgccgaaca ttcccgtcaa cagcgatttg atcgaactgc
     7321 agaacctggc catggtggcg gaattgatca ttcgttgtgc catccggaga aaagagagcc
     7381 gggggcttca ctacaacctg gaccacccac aaaccgatga cgtgaactgg cgcaaggaca
     7441 ccttggtgag ccggaccttg acggatgatg gggtttgcgt ggtttgcgac cggaaagccc
     7501 atccatgacg ccggaatgag catcggggcc gctggatgtg aggccccggt cggaacttgc
     7561 caaccgtcga ggggggcggg ccggggatgg tgcggggatg gggttttcga ttcccggtac
     7621 cgcactcatc cggtgccgag gtctcgagct tcggaggtcc ggggaacggg gccggaggcg
     7681 gggggatcgg cggaattgcc ggttgtccct tcaccggatg ggggttccag ggccggtctc
     7741 ttgggggctt cgagcagcgg tgcggctacc gcctgctcca cacgcatggt caagactccc
     7801 cccatgatgc tgatttcata gtttcggccc gagagatcca cgttctgaag agtctcccga
     7861 agcttgcgga actgcttctt cgctttgcga tagatgtaga gcagtccccc ggcgatcatc
     7921 agaaaaccga agaggaaaac ccaaaggggg gtgatgatga cctggccgat gaccatgatc
     7981 aagacggcca gtcccaccac aaacaccacc tgcacaaaga tgatcaggta gagatagaag
     8041 attccctggt tgatggaggg ttcccgcgtc tcttctttcg ggggtttctt cctgggaccc
     8101 ctgaaaaagc ggccgatatc catggagtct tgtcccttcc taagggtgcg attcttcgag
     8161 ggtcgcgttc ttttgcacgg gctcggggag taagggagag gggggctgat tctgcagaag
     8221 cagttgcttt tccactcgat acttctccag gtccagttga ttgtccaata gctgcttgga
     8281 aagtctgtag cgcaaataga cgatccatgt gatcagggat gccaagaata cggcgcaggc
     8341 cacaaagaag cccttgtact tctcgatggc caccgcgccg atctttgcca ggtctcccag
     8401 aaaattaggg acgacccaca agaggatcac cgccgccgca acgagaaaac ccacgtggaa
     8461 aacggtcaca cgccgggaaa aatcgaaaag ggaaccccac cgacccttgg gcgaggggct
     8521 caggcgggaa tccccacccc cggaatcctc ctctccgtac acaagggcca ggtaggctcg
     8581 aatgacaaag gcaatcagca gaaccagtcc gatgggaacg cccaccgccg ccgacgccca
     8641 tgcacgaaag ggaaagggga ccgtctcgct ctgaaggtgt acctggtagt ccttcaaagg
     8701 accaaaagtc ttctcgaatt cgagccgctc gtaggccgtg agggtactcc cggccctgtc
     8761 ggccaccacc ttcccctggg aatcggtgat gagaatgcgg gattgcttcc ccgtctggtt
     8821 caaggccgcc aggatgagga tgagctcgag aagcaggaac gagaagatac tgacggccaa
     8881 ggcaaccttg agttgccttt tgccttcgaa tatatccaaa agacgcaact tgcctttcat
     8941 ggctcccaat tcgcaaacag agtccatatc ccgggaatca ttgcttcccc gaagctaaca
     9001 tccggggcac ggagtgtcaa atgaaaaggg gggcggggaa aaggcgggag ccactccgga
     9061 ccgtggaggg gcgggcatca accccaaacg gaaagggcag cccccgccgg ggaagccgcc
     9121 ctttttcttg ttcagccggt atgaagcgtg tgcgccgggc ggcccggccg cccagcttgc
     9181 cggggtgtgt cgggagtcct ttcccggtgc ggcacccggg gtgccgcccc ttctcccgcg
     9241 gacctcaagg ggagacgggg ttcagttccc tctcccgggg aaagaggggc atgtagcgat
     9301 acgagaggga tatgaccagg gcgcagtacg cgatcatggc gatgcccggc gcccactcat
     9361 acatggtcgg cacatagacc tcccagtgat cgaacggcat gaccggaacg gcgattgcct
     9421 gaaccgtcat gacgaaacga ttgatggtga ccccgatgca gtcgagaatg aaggcgccga
     9481 agagcaacag gttgttcttc ctggcctggg ggatgagcag aatgatggcc gggaggaccc
     9541 cgcagacgag gatctcggtc cagagaagcc acatgccgta cggttcgcgg taaaagtcgg
     9601 tgagctggag cccgaagccg ggagccagca ccatggccca gtagagagtg tcggcaatct
     9661 tcaggatgat gtagagggcc agaaatccgc ccacaatttt ggccagcaac tcgtagacac
     9721 tccggtccac cagttgcctg ccgctcacct tctcgatggt ccggcacacc aggcccgtca
     9781 gtgcgggccc cgaggcgatg gccgacagga caaacaggaa gaaggtccag ggccagatga
     9841 aaaaccccgt ccggtaagcg aagggacggc cgaaaagaac cccggcgact ccacccaggg
     9901 acccttgatg gaagaagctc agaaacgtac ccgtcaaggc gaacaccgcc atgatctcat
     9961 ggagagagtg gccgaagaca tggaaggggc ggacggcggc gagctttcga ttctcgagga
    10021 tgatggggat gtattcgatg gtcagcacga tggcgtaaca gctgatgcag aagatcacct
    10081 ccgtcagcat ggaatgaaca ttcgcgtgcc agtaaccgaa ccaaccccgt atgggctgcc
    10141 cgatttcaag gagcaggacc gccagggcgc ccgtgtaaca aacgaacccg atcaccaccg
    10201 ccagattgat gatcttgtgg agttccttga gggcgggaaa gaaacgggac atgccgtagt
    10261 agaggaaccc cgaaaagaac gctccggcac cgagggcgat gatggccaga tcgatggtga
    10321 tgtaaaagcc gaaaccgaaa taatcattga gaccggtcac gttcagggcc ttgaggagaa
    10381 cgacccctcc ggcatacacc ccccacccga tgatgccgaa aagcaccagc aaccagagcg
    10441 caaaaacggg aagtggacac cgcttcactc cttcgggaat caacgcttta tccattctgt
    10501 acccctcgtt catgactgat tgattggtga gttcccaaga tgagggacaa cgccccgccg
    10561 gttctcaggc aaggggcccc gggactcggg gatctacagg ctgttgtccg ccagcctgtg
    10621 gacccactga tgcttgctca gatagatgac cttcggttcc gtatggagct tggccaggat
    10681 tcgaaatgcc cgtgggtcct tagccagttt ggccacttcg ctctccgggt cctgaaggtt
    10741 tccaaaggcg atggccttgg tggggcacgc ctccgcacag gcaggagtgt aggtgaccgt
    10801 cttgagatcg gcaccatcca ggatggcttt ggatcgagcc ttgttcaagc gatgattgca
    10861 aaaggtgcac ttttccacca ccccgcgcat gcgggtgctc acttccgggc tgagcatcgc
    10921 ctccatggac ttgggccagg tggggtccca ccagttgaac ttacgagcgt gataggggca
    10981 cgccaccatg cagtaccggc agccgatgca ccggggatag acgtgggata ccaccccggt
    11041 gagttcgtcc cgggtcgtgg cgttcaccgg gcagacgaat gtgcacgggg aatgatgggg
    11101 cgcatcacag tgcatgcagg gacgaggcag gtaggtcacc tgatgctcgg gatagtcctt
    11161 gccgttctgg agcgagtaca cctccatcca cgtgatcgat cgcaccttgt tggactcatc
    11221 cgcccgaaaa gaaatgttgt tctccgcttg acaggcgaca ctgcaggcca tgcaaccggt
    11281 gcacttgtca aggtcgatca ccattccata gcgagcaccg ctttgtgtat gtccatgttc
    11341 actcacaggt tacccccttt ggtgatgcaa ttgcgcgcga ttggcccaaa cggttcccag
    11401 attggtcacc ggatccagct ggacctcgac gacctggttg gaattcactc ccttgtcccg
    11461 gatatactca tcgtaggcct catggccgag ccctcgggcg atgtagacca cccccgccct
    11521 ggctgcggcg gacaagtgga ctcgaaccga tgccttcccc ttgggtgttt caaggaccgc
    11581 gcgatccccc tgtttgaagc ccagggatcg ggcggtctgg gggtgaatct ggacaaacat
    11641 gtcgttgccc agcagctgag tatcaaacag agtcttgttc agaaagggag gattgggcag
    11701 gaacccgccg gccagattcg aggccgggtg ggccaccagg tacagaggga agtccttttc
    11761 acttccccgg ggcggctcca gggccaaatg gggcaggtac ttcctgtctt cccccacaaa
    11821 gcccttcttc aggagggatt gacatgccag ttcgtagtct ccggaatccg tctcgatctc
    11881 ctgcatggaa ttgacagggg cgttcaccca gcacaggccg cccacgagct tcttccagag
    11941 gtcggttccg tccttgaaat tgggggtgag gggttggcct tcctcgagct tccacgggac
    12001 gacctccttg ccgtcgatca ccacgccctt ccccgaagcc gccaatccct tgacccgccc
    12061 ttgcaggaaa tcctcgtagc tcttccacgg cagggctgct tccatgcccc ccgccaggtc
    12121 cttggccagc tcgatgacca cgtcccccgt gtgcctggta tccagtcggg gtttgaggac
    12181 cggggccgcc acggcgtaat aggcataggg agctccgacc attccgatga cgtcgtcgta
    12241 tcgttcaaag gcgctgtgat tgggaagaat caggtccgcc tggaccgaag tctcgtccag
    12301 ataggaagag aaagaaacca ggaagccgat cttctcgaga gcggcataaa aaagaccgtt
    12361 ctcctgaagt tcataggcgg gattggcctc gtgaaccatc aaggtgtcga tgggataggg
    12421 accgccggtt gccgccgcgt cgaggaaggc atggaggccg tttcccggca gcgggacctt
    12481 gggggatttg gccaggtcca ggcgaagggg agggggtccc ggggcgccgg acgagatttc
    12541 gggcaacggt tcaaagggta ccctggggac aaagctcacc aagccgccgg ttctcaggtt
    12601 tcccttgaga acgttcaggg ccagaaaaac caggtcgctg taaatgtttt cggcgcaatc
    12661 cccggcaccg gagccccaga cggcgacggc gttcttctgc ccggcaaact cccgggccag
    12721 ctcccggatc ttgcccgcgt ccagccccac ctgctcggcc acttgctccg gtgcataggc
    12781 ggaaagcagc aggttcttga atccctggcg ggtttttccc gcggcgtcgg tccagtcttc
    12841 gaaaccgaag gtcgaggcgg ctatgaaatc cttgtcgtaa aggttttcct tcaccatgac
    12901 gtgagcgacg gccagcgcca gggcgggctc gctccccggg gccaccgcca cccatcggtc
    12961 ggccttggat gccgtcatgg aacaacggga ttccacctgg accactcggg tgcggccccg
    13021 ttcgggcagg tcctgctgcc acagggcgaa ggccgtctgc attctgccgg gggagcccca
    13081 tccttcaatg aggttggaac cgaagctcag cacgtaggag gctctttcca atgcaaagcc
    13141 cacatccctc ttttgtcccg tcgtgagctg gaccgccagt tgtaggctgt tgccgtggga
    13201 aggcattcgg aagaggttgg gagagccgta ggagtcgaaa aaccgttccc acagaagact
    13261 catgctgctg cagcgggccg aggcgatgca ggccgctgcg gccgcttttc cctcggacct
    13321 gagcttactc aaacggccgc ccagctccgc cagggcatcc ttccaggaaa tgggctggaa
    13381 gcctttggcg tttccccgtt tgctcacctg tttcatgggc tgggagaccc ggtagggagc
    13441 gtaaaggaat tgggccccgg cggctcccag agggcaaatg cccccctggt tcaccggatg
    13501 tttggggttt ccctccacgt agataacgcg ggtcccgttg acgagccggg cctgaatgcc
    13561 gcatccgccc tcgcacaaca cgcacacgga agactttctc gtgatggggc ctctttccgg
    13621 ggacgggcgc catgaccagt tctggctcca gatggcactg tcatccatca atttccaggg
    13681 aatgggggtc aataaggaac cgcccaccgc accggcggca aactgtaaga aagctctcct
    13741 cccgaatttc atgccgttta ccctctcatc aggactatct ggttgggtcc caagggaccg
    13801 ggtttcccaa gttcccgggg cattccgtcc cggctacttg tggcatatcc cgcaatcgtt
    13861 gttggcggac tgagcggcgt ggcattcctc gcactcgatc atcttcatgg tgccctcgct
    13921 gtagccggtc agacgattct cccgatacgc ggggagcttc tgttccttgg ccacatctct
    13981 gtggcagcgt acgcagggga cgttcttggc cttgtgggca gcatgggaga aaaagacgtt
    14041 gtccggctgg tacgcgtaga cgagccaggg gatctcccgg tccttggcga tgtactcctc
    14101 taccaggatc cgttcggatt ccgactgccc cagttgggac tcatgacatt ccttgcactt
    14161 gtccagctgg ggaatcccgg tgtaggtgcc gtcgggcctg aaggcgtgac agtcttcgca
    14221 tgtgctgtcc tggtgggccg catgactgaa gttgatgggc tggatccttt cggaaaagag
    14281 gagggaggga aagacgatcc atcccaccgc cagggccacg ataaaccccg agaagaagat
    14341 gactaaccct aagacactac tgccggaaga atgcttttgc tcttctttca taggactgcc
    14401 tcggttgcgt cacggtatgg ttgattgggt cgggcgtccg gcgcccttgg ccggccgccc
    14461 cctcctaacg acggttttcg ccctcattgc gaggagttat gcaaaaacaa attttcacaa
    14521 gggagactcc tctattaatc gcatccctgg gaccttgtca agagaaaagc aaggaaagaa
    14581 aagcctgcgg gaaggcagga gcgccctgta tcgggaggaa acccagcagc tgtggagatg
    14641 gtggacggcc atgaaatgtg cattccggat tgcacaagag cattgagaac gggatcgaga
    14701 gagcctgcac cctcaattct gcacggcccc actcatagga cgggaatcaa ctgaagtcaa
    14761 catttttttg atattctcaa actgtggaaa ttccgggggc ttggccgtcg gcaagagact
    14821 gcgcccggcc ctcgggaccc ctgtgaaatc atttcgagcg tgcacctcgg gcgagttgaa
    14881 tgttatgata acgtccggcg cggacaagcg gggcttgtcc gcgacctttt cccggcaacc
    14941 gccgatgccc gaacctctat cgaggaattc ccgtgatgag ccctcggacc cttccggcct
    15001 gttttcgagt catgaccttc aatctgcgtt tcgagaatga tcgggacggg gaccatgcct
    15061 ggagcctccg caaggacttt gtggtggagg tcgtcaaggc ctgcaaaccc gcgattctgg
    15121 gcacccagga aggaacgccg ggccagctgg attttctccg ggagcacttg ccggactaca
    15181 cgatggcggc gcccgatcgg ccctttgacc ccacttgtca gtatccgacc ctgttttacg
    15241 agaccggaag gtttcaccgg gcggacagcg gcgaattctg gttgtcccat acccctccgg
    15301 tccatcgcag caagagctgg gacagcgcct ttccgcgcat gatgggttac ggtttgctgg
    15361 aagaccggga ggttgggaaa accttctacg tctcggttac ccacctggac cacctgggtg
    15421 ccgaggctcg agtcggccag gcccggatga tcgcggaatg gattcgtgcc caccccggtc
    15481 ctcacattct catgggagac ttcaaccagc caccggattc aagggtccac gagatcttga
    15541 ccgacccccg gatcggtctt ctcgacacgt ggcaggtttt gggaaaaggg cagggcccgg
    15601 aggggtggac tcaccatgat ttccacgggg tccctcacaa attccgaatg gactggatcc
    15661 ttgccgggcg gtcttttgac gtggtccgag ccgaaattgt gcgggaccac aaagacggcg
    15721 tctatccttc ggatcacttt ccctactggg cggacctgtg ctgggcaagc taagatcagg
    15781 agggaccggg ggggtggtcc cttgtaggag ctccggcacc gccggggcac ggcccttgag
    15841 gtcggtatcg gaccggaaac ggttttagaa acagaatcgg aaacctttgg gagtcgtcat
    15901 gaaagagagt atacgagtgg ccctggttca accgaaaccc tatcccgctt tcgatgatcc
    15961 caggaacctg gggcacgcgc tgctgctgtt ggaaaaatgc cggggggaat ccctggacat
    16021 ggtgtgtttt cccgaatact tccctttcca gggggaacgt gagctggcgg cggcggcaaa
    16081 agacctcaac gcctaccttg tggccggact ggtggagtcc gaggggggaa agcaatacaa
    16141 caccgcgacc ctcattgacc gctccgggca catcctgggc aggcagcgga agcgcaacat
    16201 cgggttgatc gagcgggaag ggctggggat ctcccccggg gatgggatct ttcgagcctt
    16261 caccacggat ttcggcaaga tcggggtccc ggtctgtatc gatttctggg gacagcccga
    16321 ggccggaaaa caactggcgg atcaaggggt ggaggtggtc ttcaacatca gtatcttccc
    16381 cattttgcga gggcactgga aagtgggcgc ttccgttcga gccttcgaca atttcttctc
    16441 cgtggtggga gtgaacaccg ccgactacaa cgctctcatc cacgggaagc ggattcacca
    16501 tcacggagga aagagcttcg tcctgcagcc tcccaagctc ctggacaaag acgatttccg
    16561 ccgctggctg cgcagtctcg actcgacgga gaactggatc agcctcgagc tcaacgaact
    16621 ggagcaggtg gcggtggcct ccgtccattt gagcaccacc cggcgctttc ggcgcgaatt
    16681 ctggaatcga ttcggtttcc aacgctgaag cggatgtcgt cccggggaat tcggccatgt
    16741 ctcccctggg ggttggccgg gacaaagaag ggacccgtgg cttcgaggat atggggcctt
    16801 cgacgccccg ccgggacctg aaccgggcat gccgtccgga tgccccccgg gaaaactgca
    16861 tcctttggtt ttcgtggagt acgaagtatg aaagagctga aacgcaacat ttacctgtgc
    16921 atgaagaccc tcgatgaggc cgagcgaatc tggggggacc gggtttcttg cctgcgaacg
    16981 aggctggaaa gggttccgac tgctcaggcc cttcatcggg tcaccgggga gcccctgacg
    17041 gcccggcggt cggttcccca ctatcacggg gccgccatgg acgggtatgc cgtcaaggcc
    17101 cgggccactt acggcgccgg ggacgttcgt cccatgatcc tgaaggtggg ggagaacgcc
    17161 tttccggtgg acacggggga ccctttgccc ggggataccg acgccgtcat catgatcgag
    17221 cacgtccagc ccgtaaagca ggacgagatc gagattcgtg ctgcggcgtt tccctggcag
    17281 catgtgagga aggtcgggga ggacatcgtg gcgggagagc tgatcctgcc cctcaatcac
    17341 cttctgcgcc ccgtggacct gggggctctg cttgccgccg gggtcctcga agtccccgtc
    17401 tacgcgaagc ccaaggtctg gatccaacct acgggcacgg agcttgtggc ggcggaggaa
    17461 gccgccgaat cggcccctgg gcaaattgtg gagttcaacg gggtggtgct ttcgggcctg
    17521 gtgagagagt gcggtgggga gccgctcctg cagccgatca tcgacgaccg gtacccggtc
    17581 ctgcgggagc gcatggaagc cgccctggcg tccgaagccg acgtcgtgct cttcaacgcc
    17641 gggtcttcgg cgggcacgga ggactacact gcagccatcc tgggggaggt gggggaggtc
    17701 cttgtccacg gggtcaccat gatgcccgga aagcccaccc tgctggggct ggctcgggga
    17761 aaaccggtgg tgggaatccc cggatatccc gtttccgcac tcttggcctt tgaacaattc
    17821 gtccgccccc tgttgttctc cctgcaaggg gtgtcggctc ccgcctttcc ggaaacggaa
    17881 gcgattctgg gacgcaaatt gccgtcccgt ttgggtctcg aggaattcgt ccgggtcatc
    17941 ctggggcggg tcgcggggaa gctcgttgcc atgcccctcc aacggggagc cggggtgatc
    18001 acctccctca cccgggccga cgggatcctg cgaattcccc aggagctcga aggaatggag
    18061 gtggccgccc ccacaaaggt tcgtctcctt cggtcccgag aacagctgga ccggaccctc
    18121 atcatgatcg ggagccacga caacaccctt gacgtgctgg ccaatgagtt gaagaagcga
    18181 gacagccggg tacacctgtc ctcgagcaac gtcgggagcc tgggcggact cctggccgtc
    18241 cgccaggggc attgccacct ggccgggtct catctcctgg ataccgtaac cggggagtac
    18301 aatttctccc acatcgagcg atatctcaaa gggacaccgg tgcgcctggt acgattggct
    18361 gcaaggaccc agggctttct actgaagccc ggaaacccta aggggattcg gggggtccgg
    18421 gacctggttc gcccggacgt caccttcctc aatcggcagg cgggatccgg gacccggatc
    18481 ctcctggact atctgctgca aaaagaggac ctggcttccc aggggattgt cggctacgat
    18541 caggaagaat tcacccacat ggccgtggcg gtcaacgtgc tcagcggccg tgcggacacc
    18601 gggatggcga ttttcgccgc agcccgagcc ctggacctgg acttcgtccc cgtgaccgag
    18661 gaacaatacg acctggtcat ccccgagtcc gaatgggagg accccaaggt ccggatgctc
    18721 ctggacattg tcgtttccgg tccctttcgt cggacggtgg ccgccatggg aggctacgac
    18781 acggctcgtt cgggcgaact ggtgggccgg tgggacggtc gggaatggtt gttgcgggag
    18841 cgaacctgat ccgacatccg cgccggaccg ggatcgggaa cacatcccgg aatcacaggg
    18901 acaacccccg gaaggtaccg gacatgttcg gacggaaatg gagaagaaac gttgctctcg
    18961 gccttgtcct cccggcactg ctgctggtcc ggagcccggt cgggtcacag agcggtgaaa
    19021 cggcggatcg cccgagcgag gtcgccctgc ttccccgggc gaacggcatc aagaacctca
    19081 tccgggtgcc cctggttcgc cagtcgaaga attatacctg cggagtcgcc cccctggagt
    19141 cggttctcgg gtattcccgt cagtccatcc cggagggtaa gttggccgag aggctaaaga
    19201 ctacggaggc cggggggact cattatcgga acatggtgcg ttttgcccgg aatcacgggt
    19261 acatggttcg caaggttttg gacatggccc tggaggacct cgagcaatgg atcgacagga
    19321 gaagaccggt gatcgtggtc atccaggcct gggccgatcc tcccgtggat tatccccacg
    19381 attggaacga cggccactac gtggtggcgg tcggttatga cgacgaaaac ttctacttta
    19441 tggacccttc caccctgggc cactatgcct tcatttcgcg ggtggaattt ctcgaacggt
    19501 ggcacgacaa ggacgacacc acgggggaag tcttgaatcg gtttgggctg gtgctggaga
    19561 aggacggcaa cctgtacgat ccggagatcg tgacgcggtt gaaatgatcc atggctcggg
    19621 gaagtgcgtc ctatgtccgc ttgtatgaaa acgggatcct gcaggaacga gcccgaatcc
    19681 tgcgccggat gatggatcgg tgcaccctgt gcccgcgccg ttgccgagtc aaccgaaacg
    19741 aaggtcggac gggggcgtgc ggagtggcga acgggcctac cgttgcggcc gtccatctgc
    19801 atccctggga agagccccct ctctcgggca ctcgtgggtc cggcaccatt ttcttctccg
    19861 ggtgcactct gcggtgcgtc ttttgccaga actatcccat cagtcaactg ggggtgggac
    19921 gcattctttc cgtggaggag ttgggacgca aaatgctgga gctccagggg aagggtgccc
    19981 acaatctgaa cctggtgacg gccacccagc agatgcccta tgtggtcgag gccctgctcc
    20041 atgccgttcc acgcggcctg tccctcccct tggtccataa taccggcggg tacgaaaggg
    20101 tggaggtcct ccgcctgctg gacggcattg tggacatcta cctgccggac atcaagtacg
    20161 acgatccccg ggcggcggcc ttctgctccg ggcgggaaga ctatgtgagg gtgaatcggc
    20221 acgcgctgct ggaaatgtgg agacaggtgg ggggattgca cctcgattcc aaaggccttg
    20281 cttttcgggg aatgctggtg cgccacctgg tgttgcccgg ggatttgtcc ggaacaagga
    20341 actgtttgcg ctttcttgcc gcccgattgg gtccgggggt gtgggtgagc ctcatgaacc
    20401 agtatttccc ggctcaccgt gccctggaaa tgccacccct ggaccggaaa gtcacccggg
    20461 aagaatatga aagggccaaa gcctgcctcg atgagctcgg gctggttcat gggtttgtcc
    20521 aggggggagc ggaaaaggag accgggactt ttcttccccg gaatcccgct ccccggaagc
    20581 gcggcaggaa aagggaagtg ccgggatagg gtggttcgcc gggaacggcc cggtgcagga
    20641 cgaaccttgc ccgaggcccc caaggtgggg gcagggcggg aggcgtgacc atgggaccta
    20701 ccgtcgtgct gttggtggac gacgaagctc cgttcgtgga aaccttgacc cgtcgattgg
    20761 cccgaaaagg cttcgtcatc ctgaaggcgt tcagcggcct cgaagccctg gatggtctct
    20821 ctcggcgcag ggacgtcgat gtggtcctcc tggatgtgaa gatgccgggg atggacggca
    20881 tagagacctt gagggccatc aagaggcatc atcccctggt cgaggtcatc cttttgaccg
    20941 gtcatgccgt cctggagacg gccatcgagg ggatgaagct gggagccttc gatttcctgt
    21001 tgaagccctg cgagatggag cacttggtgg gcaagatcct ggatgcaaag gacaggcgga
    21061 gggatcgtgc gggaaaggtg ttacaggctc gagccgtcga ggcggctgat cgccgggggg
    21121 attgagggag aggactcagg gggaactctc cctccggtct catgttccgg gcttcccggc
    21181 attgtggtta tcccacgggg ggcgggtcct acggggtttc gagctcgagc ggttctcttt
    21241 cctcttccac cctttccttt tgtccaaaca tggtctaatg tagcagctct cggttctttc
    21301 cagcatagac attgtcccga aatccataga gaaatcctgc gtaagggcct ctcctgcgcg
    21361 ccaggcaagg tggacaggca tggaagaaaa aggatacaga aagctgcagt ggaagatcat
    21421 cgccacaact ctcggcttcg ccctggtccc cctctttgcc ctgggcctga ccatctacta
    21481 tcagttcagc gtctcctaca cagccaagat catggagagc ctgaaaactc tggcggaaaa
    21541 ccggcgcagc gccatcgatc tttttctgga cgaacgggta tcccagctca attccttggc
    21601 gtatacccac cacctggatc agctcaagga cgaagaatat ctcaatcgcg tcttcaccct
    21661 ggttcaggcc aggtccaagt cctttgtgga tttcgggatc attgaccggg aagggaacca
    21721 ggtggcgtac gtggggccct accagttgaa ggggctcaat taccggaacg aagagtggtt
    21781 cgacgccgcc atgcttcgag gggtgtatat cagcgacgtg ttcatgggct tccgcaaatt
    21841 tcctcatttc atcatcgccg tcatgcgacg agagggggat cggtcctgga tccttcgggc
    21901 caccatcgag acggaaatct tcgacaccat ggtcaaggcg gcccaggtgg ggcaaaaagg
    21961 ggacgccttt ctcatcaatc gagacaaagt gctccagacg accccgcgct tcggcggaga
    22021 ggtgctggat cggctcccgt acgggaattt caccaagttc gagggaactc gggtggaaga
    22081 gatggaggtg gacgggcagg cggccctatt cgggatgacc tggataagga acaaggactg
    22141 gctcctggtc gtccgggagg atccccggga agagctgtcc cctttgtttc gagcccgcca
    22201 cctggccatc gccctggtcc tgggcggagt gctcgtcatc gttttggggg cgaccttcgt
    22261 ggcccgggtc atcaccgccc agttgatcca ggcggaccgg gaaaaggcaa tgctggacgc
    22321 ctccctcatc cagtcgagca agatggcggc cctgggcaag attgccgcgg gaatcgccca
    22381 cgaagtgaat aaccccctgg cggtgatcaa ggaaaaagtg gggtggctga gggacctcct
    22441 ggaggaggaa gaaatcgcga ccaatgaaaa ctacaaggag ttcgacgacg ccgtacggaa
    22501 aatcgactac cacgtggacc gggccaagaa ggtgacccat cgcctcctgg gtttcgcacg
    22561 gagaatggag ccggtacaag agaaagtgga cgtcaaccgg ctgttggaca cgaccatcga
    22621 tttccttcgc aacgaagccc actaccgcag catcgaaatc cagacggact tcacggaaac
    22681 cctgccccag atcaccagcg actccgccca gttgcagcaa gtcttcctca acatcctcaa
    22741 caacgccatc gatgccatcg gcaagaacgg aaccatcacg gtccgctcaa gactcaccaa
    22801 caaggacaag gaaatcgccg tctgcattgc cgatgacggt cccgggattc ccagcgacgt
    22861 gctgggcaag attttcgatc ccttcttcac caccaaggag gtcggaagcg gaacggggct
    22921 gggtctttcc atcagctaca gcatcgtgga gaaactgggt gggagaatcg tcgtagccag
    22981 ccaggtcgga aagggaacca ctttcaccat ctgccttccc gtcaatcaaa gcgcctagca
    23041 aggatttgcg gaacgggaag cccttgccgg cggccgtagt ccccctgccg tccggccctg
    23101 gaacgacaag acaagaaggt cgcagcatgg aaaattttag ggtcttgatt gtcgatgatg
    23161 aaccggattt tctcgaaacc atcgtgaagc gtctcctcaa aaggaaagtg gacgccacgg
    23221 gggtggacag cggtccgaaa gccctcaagc tcctggagaa ggaacccttc gatgtggtca
    23281 ttctggatgt gagaatgccc ggcatggacg ggatcgaaac cttgagggag atgaaaagaa
    23341 agaatcctct catggaggtg atcatgctca cgggccacgc ctccgtggaa gcggggatgc
    23401 agggcatgca gctgggcgcc ttcgactacg tgatgaagcc tgcagagatc gatgatttgt
    23461 tggaaaaggt tcgtcaggct tatgaacgga agtcgctcca cgaggagagg cttcgtgcgg
    23521 agggttgacg ggagcaacgg gtcccgcacg cgtcattgtc acggttgatc ccaacggcgg
    23581 ttgatggcag gtcatgtcga acctttggcg atccgtttat ccgttcctgt gttcccatgc
    23641 cttgagcttc ctggcgttgg gcctgtccgt gcccgtctgg gcttttggag gatggtccgc
    23701 ctgggtctgg attctcccca tgatcgcggc ctccgagatc gtgatccggg attttcaggg
    23761 gcggatccgc cgggccaacg aacagaaatg ctttctggac gaacagttga tccagtccca
    23821 gaagctggcc tccattggag agctttcctc ggggattgcc cacgaaatca acaatccgtt
    23881 ggccattatc gggcaggaag tggaatggat ggggcacctt ctgaagaagg agagcatcgg
    23941 agacccccgg gagatggagg aactgaaggg ctccatgaag gaaatctccc gccaggtgga
    24001 tcgctgccgg gaaatcactc ataagctcct ggattttgcc cgcaagaggg aaccgctcct
    24061 ccagggggtg gacatcaacc gactggtgga agacatggcc cggctggtcg agcgggaggc
    24121 aacccatcac gggattcgga tcgtgcgcga ataccgcaag gacctgcccg tggtctacac
    24181 ggatgcgccc ctgatccgtc aggtgatcct aaacctgctc aacaacgcca cctatgccgt
    24241 tcagaaggac ggcaccatca ccgttaggac ccggcgggcg gaccccgagg ccatcacggt
    24301 caccatcgag gatacgggtt gcggtatttc caaagaacat ttaaacaaaa tattcgatcc
    24361 cttcttcacg accaagcccc ccgggaaagg gacgggactg ggcctgtccc tgtgccacgg
    24421 catcgttacg aagctcggag gccaaatctc ggtgagcagc gaggtgggga agggatccaa
    24481 gttcaccctc cgtcttccgg cgcaatcgaa aaaaggagaa gcatgagcat ggagcaggcg
    24541 tcgaggaagc caagagtcct cctggtggat gacgaggaga gatttcgcac gactttggga
    24601 aagttgctga ccgttcacgg actgtcggtt tccaccgttg gaagcgggac ggatgccctg
    24661 gaggaattga aaaagggcgg ctacgatgtg atcctgttgg acgtgaagat gcccggtatg
    24721 gatggaatga cgaccctggc ggaaatcaag aagatcgacc ggaacctcga ggtcatcatg
    24781 ctgacgggac acgcttcggt ggacgtggcg gtcgagatca tgcgcctggg cgggtacgag
    24841 tatctgctga aaccctgtcc cgtggaggag atcctcgaga aagtggaagc ggccttcgag
    24901 cgaaaagcca cgcgggagtc ccggttgaag aaaggagccg ctagtccggc tccgaccgtt
    24961 tgaacgcctt ggactgaatg ttggtgtcct tcatgagtcg gtggagatac tggcgtttta
    25021 ctcccagctc ccgggcggcc tgggaaacgt tcccgccgtg cttcgagagc accctgtgaa
    25081 tgaatcgcga ttggaactgc tcgatgagcc gttccttcgc ctcttttagc ggaagggaga
    25141 acaactcttc ttccccgggc cgagccgtgc ggggggaggg agccggggat ccgggatcgg
    25201 acaaatagag gtctcccagg cggatgatcc cgtcctgggc catgatcacc cccctctcga
    25261 cggtgttctc cagttcccga acgtttcccg gccaaggcct ttccatgagg tactcgagga
    25321 cgccttcgga aaaccgggcc tcctttccgt gaagggcccc gtatttttcc aggaaatggt
    25381 gggccaggag cggaatgtcc tccgttcttt cccgaagggc gggcagacga aggttgatga
    25441 cgttcaaccg gtagaacagg tcctcccgga actctccctt tcgaatcttc tcttccaggt
    25501 tgcggttggt ggcggcgatg aaacgaatgt ccaccttgcg gatcgaggtg gatccgacca
    25561 ccttgtattc tccctcctgg agcagccgca gaagcttcac ctgcatgggc gggctcaaat
    25621 ccccgatctc gtccaaaaac agcgtcccgc cgttggcctc ctccaccagt cctttcttgt
    25681 cccggatcgc ccccgtgaat gagccccgca ggtggccgaa gagttcgctc tcgatgatgg
    25741 attcgggaag agtgctgcag tcgatgggaa tgaattcctt gtcctttcgc agactgtggg
    25801 tgtggaggat gcgagccacc agttccttgc ccgttccgct ctcgccggtg agcagaaccg
    25861 tggccgaagt cttggctacc cggtcgattc ggctgtggag cctctggatg gcggggctgt
    25921 ttccgatgaa gctggaaaag ccggattctc ccgaaagcct ctcccgcagg taggagttct
    25981 cgcgctgcag gcgctgccac ttcacggcct ggtccagaag gtggaggatt ctttcttttc
    26041 gaaagggctt ggtgatataa tcgaatgctc ccttccgcgt cgcttcgacg gcggattcaa
    26101 tggagccgaa tgccgtcatg accaccaccg ccgtgagggg gtccttcgct ttcacgcatt
    26161 ccaacacctg aatgccgtcc atccggggca ttttgaggtc ggtgatgact acgttgtagg
    26221 catcggcctg cagcaggtcc gggactcgaa gaggatcgtc ggtggtcacg acctcgtagg
    26281 aggtcttctc cgagacgatc cgcttgagaa gggccaacat gtccttttcg tcgtcgacga
    26341 tgagtatctt cccggccatg gtgatgttct ccgcgaggtt ctcgatcgtt tccaattctg
    26401 agccccaggg gagaacaagt caaccggatt gtcatcgcat agtttcactg taatgcaaga
    26461 gggacattgt gaaagaggat agcaaaccgg aggggatggc cacccgggag agggtcaggc
    26521 tgcgtcgggt gatcaagatc gtaggaatga tcaccgtggc cctggtgggt ctggctttgt
    26581 acttgggcgc ctccaccgcc cgctacatga aggagatcat ccgcagtcag ttcaacaacc
    26641 agcagctggt cttcgccagg accagcgccc tgaagatcga gaccagcatt caaaacgccg
    26701 tgacggacct gattcttctc aactcctccc ccgccatcca gtactgcgac cccgaagcct
    26761 acgaggccct tctcctctcg actcttcccg tgctcaaccg ggaccacatc caggagatcc
    26821 gccgggtgga ccgggaagga aacaccatgt tcgtggccga tgagcaggga gtcggcatga
    26881 aacacctggg gcccttgcgc cgggaagcgg gggtgtatct gtcttgggcc tccgacatga
    26941 gcaatcgcgg caagaccatg gccaccgggc tccgcaccca ggagctgtcc aaggacagaa
    27001 agcgaatcct catcgatttc gtggttccca cctacgagga ttcctcagat ccccaacacc
    27061 cgaggcccac tcatcgtttt gccggctttc tggcggccac cctgaacgtg agcagcctgc
    27121 tccaggagat catgcccccg gtccggtcgg ggaagacggg ctacgcctgg gtcctggact
    27181 cctcgggaag cttcctttac catcccgagc cctccttcgt cggggaaaac gccttccaag
    27241 tccgcgcttc ccgaaatccg aagatctctt ttgaaagaat caatgaaatc caacgaaacg
    27301 agatgctcca tggaaaagag ggcacaggga cctatttttc cggctggcac cgggaattgg
    27361 tggaacccat ggagaagctc atcgcctacg ctcccgtgcg actccagggg ccatacatgt
    27421 cctacctgtg gtccgtggcg gtggtggccc ctgcggatga aatcgagggc gtcattcatt
    27481 cggtctacca gggtcaattc attctccagg ggatcatcat tttcatcatc atcctgggaa
    27541 ccgtcatcat catcctgtac gagctgcgct ggtccacgat cctggaacac gaggtggccg
    27601 tcaagacacg ggatatcagc cgatacgcct cggagttgga acgctccgag gccaagtatc
    27661 gctccctggt ggaaagcgcc gaggacctga tcttcatggt ggaccaggac ggcatcctca
    27721 aaacggccaa ccgccacatg gcccgcatgt tcggcatgga agacgaagaa atcgttgggc
    27781 agagcctgta ccggctcttg ccccgggagc aggcaaattc ccaactaaac ctggtgcgcc
    27841 gggtcttgcg cgagggccgt cgagtacgca cggaaaccct tctttgcatc ggcgaggaag
    27901 acttctggtt caacatccag tacctgccgg tgaaggggga ggcggggggg ccttccttca
    27961 tcctgggaat cgcccgggac atcaccgagc gcaagggcct ggaaaagcag ctcatcaaca
    28021 cggagaagct cgcttccctg gggaccctgg ccgcgggcgt ggcccacgaa atcaacaatc
    28081 ccctggggat catgctgggt ttttgcgatc ttctcctgga gcggatggat ccgggcacca
    28141 tggaatacaa cgacctgaaa accatcgaac gccacggact ccactgcaaa agcatcgtcg
    28201 aacgcctgct gagctttgcc cggatcagcg aggaaacgga agaattctgc gatctcaacg
    28261 ccaacgtgga atccatggtg accgtggtgc ggcataccct gagcctgcac aaaatccgcc
    28321 tcgagaccca cctggacgag tccctgcccg cggttcgagg ggattccagg ggaatccagc
    28381 aggtgctcct gaatttgatc aacaatgcag tgtacgccat ggacggccag ggactcctga
    28441 ccatctcgac tcggccgagc aggaagagga accacgcgga gttggtggtc gcggataccg
    28501 gctcggggat caaaagagaa ttcctgcaaa agattttcga tcccttcttc accaccaaga
    28561 aggtgggaga cggcacggga ctggggcttt cggtgagcta cggaattgtc tccaggtacg
    28621 gaggagacat cgagtgtgaa agctacaccc aggaagaaag acccggccgc tcggggacga
    28681 ccttcaccgt gaccctgcct ttcaaggaag gcctgccgcc cgatctttcc gaggcttctt
    28741 agaccttttt cacgggcctt cgcctccatc cccggctcca agacgccccc agaggccggc
    28801 atcccccctc caccctggca cgttccttgt ttttacccat gccgagcgtt catctttccc
    28861 aacgagacct cgcgacgcac gccgcgatgc cccgttcccc ggcactgcat cggtccgggc
    28921 gattcggtgc gggggcccga ggacaccatg gtcgacccca atcgaatgaa catcctgatc
    28981 tttgatcccg accgagacat gagcgagctc tttgcccggg ccctggagac ctgccgcggc
    29041 agcaaatgct accttgcctc ggaggaagac gaggtcctgg cacttctaaa ggaggtcgca
    29101 ttccgcctgc tcctggcgga tctcggcctc atcatggtga atgatttcag cctattgcgg
    29161 aaggttcgcc gcctgttccc ccaggtggtc gtggtggtgg acacgttcct ccatcagagg
    29221 gacggggtct cccgggccat ggaattgggg gccctgggct acatcctaaa acccgtccag
    29281 atcgaggtct tccggaagaa aatccgagac tttttgtcga ttcggcatcc cgggacggac
    29341 ccggatgtcc accagtagtt gacggatctg tctcccgtgg gtttctccga actggatcgc
    29401 acaagatttc gagtgccgtg ggaacgcttg taatgtcttt cacgacattt cggggggatc
    29461 gcccccggca ttaggcatac aacttgctcg atgaggctgc ccggacggga ggcacgtccg
    29521 tccggggggt cggaagcgat ggagatgggg atacctgcga taggaggaag gaaatggcga
    29581 aaaaagtact ggtgccgacg gacggctcgg aacattccat gaaagcggtg cgtgcggccc
    29641 tggaattggc ggagcagggc ctggccgacg tgaccctgat ggcggtggcc tattacatcc
    29701 gggaggattt cgaggagatg ccccccaatg tgcaggacaa actggatgcc caggccaaga
    29761 aggccctgag ggccgccaag gccctgttcg aagagaaagg gatctcggtg gagaccatcc
    29821 tggaaacggg cttcgtcccc gcaaacaaca tcatagaaag ggccgaaaag gggaagttcg
    29881 accagatcct catggggagc accgggatga cgggcttttt caagcgcctg ctcatcggaa
    29941 gcaccgcatc caaggtggtg tccacggccc cgtgcacggt aacggttgtt cgatgataca
    30001 ggaagaaatc cagagggatg caaaagggag gatagggaaa atgattggaa tccggacaaa
    30061 gtggctggga attcttgccg cggtcggcgt cttctttccg gctgcggcca tggctgcggc
    30121 gggccccgcg gccccgctcg tcatcgtggc cgacacccga aagctcagcg gaatcatggc
    30181 atggtgggcc aatctctaca atgaaagtca cctgtatttc acccttctca ccgtggttct
    30241 cattcccatc atcggcgtag tcttcggggt ggttgccgac atcgtcatga cccacatcgg
    30301 catcgacctc aaatccagag accttgccga acattgatgt cgaacccggg agcggcatgc
    30361 ccgaaatctg agagacagga ggagagacat ggattggctt tacattttga tgcccatcgc
    30421 cggggtgaaa atcttctggc cgggtttgat catcctggga atcggcgtgg gaatcatcgg
    30481 cggcttcttc gggatgggag gagcctggat ggtgactccg ggcttgaaca ttctgggttt
    30541 tcccatggcc tttgccatcg gaacggacat cgcccacatg gcgggcaaat ccctcatttc
    30601 caccatgcgc cacggcaaat tcggcaacgt ggactaccgc ctgggcctga tcatgctcgt
    30661 ggggaccgtg atcgggttcg aggtcggggc ccagatggtc atgtggctcg agcggatcgg
    30721 gagcgtagag attacggtgc gctatctgta cctggctctg ctcgctctca tcacctggat
    30781 ggtgttcagc gacgtggcca agcgcaggaa gaaggagcgg gaagccaggg ccgcggggat
    30841 cgagctggag aagacggcca cgggactcga gtggcacaag accttccaca agatcaagat
    30901 cccccccatg gtccatctca aggccgcggg gatctactgt tccgcctggc tgcccatcgg
    30961 ggtgagcttt ctcacgggct ggctggcggg aatcctggga atcggagggg ggctcattcg
    31021 catgcccgcc ctcatctaca tcatcggctg cccgacccac gtggccgtgg ggaccgacct
    31081 cttcgaggtc atgatctccg ggctttacgg cgccgcaacc tacacctaca aaggacgcac
    31141 cgagttggtc gccgccatca tcatgctgat aggcgcttcc atcggcgccc agatcgggac
    31201 ggtggccacc aagtacatca agggttccgg gattcgcatc gcctttggtc tcgccgtggt
    31261 ggggtgcgcc atttccattg tcttgaagct ccttgccatg tatttccccg cccaaaaacc
    31321 cttctacgac gcagcggcca ccttctttgt cctgggggtg gtggcggccc tgtccatcta
    31381 catcgccgtg cggatgttcc agggagccag tgccgaggtt gcggccagga aagcggccat
    31441 gaagaaggcc gaccagggtt aagggtgcgt cgggaaaccc cggagtacgg gggctgggtt
    31501 tccgggccgt ccgcggcttg gacccccttc ggggggccgc cccctcgaga acggcgaagg
    31561 ggtcttccca acacggggaa acgtaacagg aagcagaaga gtctcaacaa acgggagatc
    31621 aaaatgggaa agaatcgaca ctgggttgct gcatggctca tgtttctctc cctggccttc
    31681 atggcgggct gcggcgaata tggaaaggtg gaccagggaa gggtgatcaa gttcgacaaa
    31741 gacaaacagt tggtgaccat cattcgggat gtcaaggaag acccgaataa tcccgactac
    31801 agccacctcc cgcccatcac ctacgagctt cccaaaaacc cggacgatgt gggagccgat
    31861 cccaaggccg ggctccggat gaagctggat gccaagaaca aggagatcgt cctatttgat
    31921 ccggtgagcg gcggcttcaa gaccctccac tacacgctcc tggaccagaa ggaaaacctg
    31981 agcaaagacg atcccctcat caaggacaag aaattccccc tggtggaccg cgagaagaag
    32041 accatcacca tcttctcggg aagacagaaa ctcctggtca ctttctcggt ccccgacgag
    32101 tacttctccc tgccggacag tgcctgggag gccggagacg aggcccgctt ttactataag
    32161 gaagacggga aggccctccg gtacatgaac gtcagcaaga cggacatttt caagaagtag
    32221 cctgtgaaat ccttggcatc agggggacgg atcgctcgag gctcttgcat gcccccggcg
    32281 atcgtccctc gaaccaggac tcgttggagg acctcccggc ttctcatgga cgaagaccaa
    32341 gctctcaggt ggagaagcag catcggaaag ctcggcgccc tggtttgcgc cgttttcctt
    32401 gtggcggtct tggacggtct cgtagcccgg tttcgagagc ctgcctatga gtacccaatc
    32461 cttgcgggcc aaaccctccc ggtgaacggt cccctcaagg aaaccccgcc cgacgcggct
    32521 gcattggcgt tcgagtcgag tgccgaaggc gtccggttgg agttcgaggc gatccaggcg
    32581 ggcttctggt tcggcaatgc cctttggcga gggacgttga ccgtggactc gggggtcccc
    32641 cccggaagct accgggtcaa ggtcgttgcc aaggcaattc ccggcacagt gctcagtccc
    32701 gtgatccaag tggtcgtgta cgcggatcct cacgactttc gattccattc caagtccctg
    32761 ttcgttcggt acctggacat ttccccgtgg tggacggcga ttctgtctct gcctcttgtg
    32821 tgcggatctc tttgtgctat cctgttgctg tcacgccgta tcgacggtct cctggctcga
    32881 gaagggaagg ctgaaatcta tcgggtattg ggtgcccccc ctgaattcga ggttgccttc
    32941 gggttgggca ctgtgcatgg gattaccgag ggtgccgagc ttgccttgct cgatcggcaa
    33001 gggatccatg tcggtaccgt gaaggtccgg gaggcttccc cggtcgatgc ggtgggtgtg
    33061 gcgcattcca tggtcgaggt gaaacccggt ttcctggtat gcgcatcctc ggggatcaga
    33121 aaggggtctg tccatgattc cggactccaa ggactcatcc gttaagctgg attctacggg
    33181 ccgtgttccc aagggagtct actccaccct ctacctgcgc tttgtcgcct tgaccctcct
    33241 gtgttcgatc gttcccctga tcctggtcgg gtggggaatc taccgctact actcccggtt
    33301 cggctattcc cggatggtgg aagatttcca gacccaggtg gaatcccatc gaaagatcgt
    33361 cgagcttttc ctcaaggagc gggtttccac ccttcgaatc ctttcccaga gtcactccgt
    33421 ggaacatctg agcagaaagg aaaacctcga cgccattttc gagatctccc agcgggaggg
    33481 ctcctacttt acggacctgg gggtcctcaa cgaccgggga aggcacctgt cctatgtggg
    33541 tccctacgac ctcatgggca atgattactc cgggaccttc tggttcaaag aggtgatgga
    33601 aaagggggtc tacatcagcg acatgttcct ggggtttcga aaggtccccc atttcatcat
    33661 cgtggtggcc cgcaccgaag gagaccggaa gtggcttctt cgagccacca tttacaacga
    33721 gtttctgagc gctctgtttg gcaacgtgag gatcggccag acgggggaag tcttcctcct
    33781 gaaccgggaa gggatctacc aaaccactcc ccgtttgggc ggtctgctca tgggccaggc
    33841 ttccctgcct atggacctgt ttcgggaaga aagcggcgtt caggtcctcg ggggaagtgc
    33901 cctgggggag gaggacgctt caccccggct ggtggtcgcc tactcctgga tgaaggaacc
    33961 tccctggatg ctggtggtcc gccaggacta cgccgaggcc ttccgggacg tcaatcacgc
    34021 caaccttgcc tctctcctct ttctgttttt gagcgccctg gccatcctgg tggtgtcgct
    34081 catcaccacc gggcacatga tcaaggtcat tcgaaagcgg gacctggagg ccgatcagtt
    34141 gaacaagcag ctctaccagg catgcaaact ggcttccgta ggcgaactcg cctcgggggt
    34201 ggcccacgaa atcaacaatc cccttgccat catcctgacg gaagcccagg tcgttcggga
    34261 cctcgaggaa gacacgccca acctcgaccc cgaattcaag tcccagctgg acgaatccct
    34321 ggaccaggtg gaaacccagg tgcaacggtg caatctcatc acccagaacc tgcttcggtt
    34381 ctcacgccgc accaagtccc tcatcgagat ggtcgatctc aacaagttcc tccaggaagt
    34441 ggtggacttg atgcacaaga aggcccaaac cagcggaatc cggttttcca ggaattttga
    34501 agaaggcctt ccacccctcc agtcggatcc ttctcagctc cagcaggtat tcctcaacct
    34561 catcaacaac gccatcgatg cccacgatgg aaaaccctac gggaccgtcc ggatctccac
    34621 gcgcacggac gagaaaggaa aaggggtgga aatccgggtg gcggataccg ggagcggaat
    34681 tgcccgggag aacctggaca agatcttcga cccgtttttc accacaaagc cggtggggaa
    34741 gggaacgggt ctggggctgt ccatctccta cggcatcatt caacggctcg gagggcacat
    34801 ctccgtggaa agtgaactgg gcaaagggac ggaatttatc ctgtttctcc ccttcagact
    34861 tccggagagc gtacgggagg gtctgaaggg agccggcaca ggagggggtg caaatgagta
    34921 aggcgaagat tctcctggtg gacgacgagg tgacttttgc caacaacatg tcccggcttc
    34981 tggccaagag aggctatgaa gtctcggtgg ttcacggggg agaggatgcc cttcgaaaag
    35041 tcgaaaacga agagtacgat gtggtgatcc tggacctgaa aatgccgggg atggacgggc
    35101 tcgataccct caaagtcctc aagaaacgaa agcccttcat cgaggtgatc attctgaccg
    35161 gccacggttc cgtggattcg gccgtggacg gcatgcacct cggggcattt gactatgtga
    35221 ccaaacccgt atatgtggaa gacctcaacg agaaaatccg ccaggcccac cagcgaaaga
    35281 tgatgcagga aagcaaagga gccgatggta ccgatgtccg atcccatacg cgttttgttg
    35341 atcgatgatg aagaaatctt cgtgcagtcg ctcaccaagg ttctcaagcg aagaggcatg
    35401 gtggtggaga gtgcgggaaa cggggcgctg gggctcgaat gtctctccca acgcgagtat
    35461 gacgtgatcg tcctggacct tcgcatgccc ggtatggacg gagtggaaac cctgaaggcc
    35521 atccgggacc gggacaccct gacccccgtc ttgatcctct cgggccacat cgagttgggg
    35581 caggtctccc aggcgctcag agaaggggcc gaggaaatcc ttctgaagcc gtgccccgtc
    35641 gacaccctca cctcggccat cgaaaacgcc tacgagcgca aggtttgcgc ccgcgatgtg
    35701 gaaaggacgt gcggggaacc cgactaggat gcggtaggtc gagaagcccc ccggaacgtg
    35761 gtaaaccggg atgcgggaaa gcctgcaggc tcgggaagcg ccctcccaag aggctccgcc
    35821 tcccggagag aagacccgga agcgagcggt ccgccgtgtt cggatcgcct tcgcaacctc
    35881 ttgcccggcg gatcccggcc cggctgcaag gatcacccac ccaaccgttt ggcgccgtcc
    35941 tcaggtgttc ccgcccccgt tggtttgccc ggggatcggt tcctcctctc ccacatattc
    36001 cagcccgtaa tcccctcgta gaaaggcctt cacaaagacc cgcacggaat cgtcgctggt
    36061 catggcggca tccatctggc gaaagaactg gaggaggctc cccagctttt ccaggtgttg
    36121 gaggatttcc tcccggggcg ccttgctcag gcgggcgttc accatgtcgt gggtgatcct
    36181 caccgaaaac ccgtactcct tgaggatatc ggcgatggcc cggatccttc ggacccggcg
    36241 tacgtagtcg gcggcccctc ccttgaacac gagggtgatg tagttcttgt tggtcgtatt
    36301 cccgcaatac gtgtccacca cgctgaagtg atatccgact cgggcggtgt agttgacgta
    36361 gttgtccgag atgagcgcgt agcaggggtc gcgaaagctc gaatcctgct cggggctcgt
    36421 cgtggcatgg cgcatcatga cggaaaggag tccgcccagg tccatggtcc tggggccgaa
    36481 ccgcggaatc tttttgtgaa acatgccctt gaggatggcg gaaaacggag cggaggccac
    36541 ctgggagggc ttgacttttc cgcctttggc ggggctcttc agtccgcccc ccaagtcgat
    36601 gatgtacaga tcgacgggga ggaacacgtc cagttgaaag ctggctcctc gcaagtcccc
    36661 cacgttgtcc cccaactgga acatctcctg ataggacttt tcatgaacgt accgggcgat
    36721 gtcgtgggga gtttggcagt ggtccgccgt gaaggtcgag gaagaggggt ccgttaaatt
    36781 gaggggggct accagatcga ccactttctt gagctgccgg tatccggggg tgtgttggcg
    36841 gtgggaagaa acctcctctt cccaggcctc gtcggcctcg gttttgagca gatccgggac
    36901 ttctccctgg tagacgattc cgctgctcgc gtccacggtg atgaggcaac cccgggggat
    36961 cgtttggaat gccttgcccg tattgagaag ggtggggatt cgacattccc gggcaagaga
    37021 tgccatgtgg ccggtggtgc tcccggcgtc ggtgacgatg gcccgggccc gggtcatgag
    37081 gcgcacgaac cggggggaag atctccgagc gacgagaatg ccgccttcgg gaaacgcatc
    37141 gaggtcttcg tcgtcggtca tgtgtacggc ggggcctgcc gccgttccgg gacatcccac
    37201 ctcgccccct cgaagaagca cggggaagcc ggggagcggc tcgacgcctc gaacgggttg
    37261 agccgccagg agcagggggc gggactgcag aacaaaaatc cgccgctggg gacccatggc
    37321 ccactccacg tcctgggtgc agccgaagtg gtcctcgagc ttcacggccc aggaggccag
    37381 gagggcggct tcttcgtccg tgaggatggg ttcccgggcc agggaaggcg acaggtcctc
    37441 ctcccgcacc cctttgccgg ggaccatgag cagctgccgg tcctggttcg aaggggttcg
    37501 aagaatgcct gtcccgtgct tgccccgggg aactcgaagg acttcggggg aggtcttccc
    37561 gtccaccagc atggctccca ggccccggac cgcctggatg agcacctggt cgagctcggg
    37621 acggccgggg tcccgagaaa acaccactcc gccggccacg gcctccacca tctccacgaa
    37681 ccccaccccc atctgggccg actcaccggg gatcccgtgg agcaggcgat aggacacggc
    37741 ttcgggggaa tagaggctgg cgacgacccg gtggtaggct tggcagagct cgtctttggg
    37801 aatgttgaga agggagagaa attgcccggc aaaggaaaag tcgctgtcct cgcccacggc
    37861 actcgatcgc accgccagga atacttgtcg gcccgtcttt gcgcacagcc ggtcgtaggc
    37921 ggaatgaatg gagtcttgta ggacggcggg gatctcgagg cccatgagcc gctcccggag
    37981 ttccccgctg actttcgtca cgtcctcctg ggactcgatt tccatgtgct tgtcctggat
    38041 ccaggaccgg atgcccccct gctccaccag cagccggtaa gcttcggtgg tcgccacaaa
    38101 cccttcgggg gtcggtagtc cgaccacgtt ggccaactcg ccgaagttgg ccattttccc
    38161 gcccacccag tctccctggg agccgttgat gtcctgaagg tccatcacca gggccccggg
    38221 tgcggaatcg gtttcctctt ccattgcccg ggtcagccgc tgggagattc gctcctgggc
    38281 accccgtagg gcctcatacc ggttgccgga aatgacgttg atgctctcca ccagggcatg
    38341 agtgtccgcc aggatccgca ccaccttccg cttgatgaag gcgcggtcga tgtattcccg
    38401 gctgatgagc ttccgttcca ggtcccccat ggtttcgaga agactgttgt tggcggagag
    38461 gagctttcgg aattccgtgt ggtacagcct cagccgggcc agctgaagct cgtggtcgat
    38521 tcggtggtct tcgcttcgaa gtccgatgat ctcgaggaag gtcaaaaagc ttttccagaa
    38581 acgtttcccc cgacccacag ccgcagggcc gtcttcccgg gaagcactgg tctcgggggg
    38641 atgggggttg cttgtcgggt ccatggattc tcccctaagg gccggggcat cggccttcgg
    38701 cggccgcctt gatggcgtcc accagcttcg tgatggtgca gggcttgagg aggtaacaga
    38761 aagcaccgga tttcatggtg tcctcttcgt ccatgggaga atagtgaccg gtgaggagga
    38821 tgacctgggt gcccggctcg atgcgcttga tttccgccag ggcctggatg ccgtccattc
    38881 cgggcatctt gacatccagc accaccacgt cgaaggactc cttggcaatc aagggaaggg
    38941 cggcataagc ggtggtggcc gagtgcacct cgaagccccg acgggtgagg accttgcaca
    39001 ggctgtttac gaaatccact tcatcgtcca ccagcaagac tttcatgtct ttgttttctc
    39061 ccggatcttt tcgcgcagga atcggagcag ttgccggccg cttttctttc ccaccgcgtg
    39121 aatttgaacg gggacggggt tcggggaagg gttgctcgag tccagctcga tccaccgggt
    39181 gggcaaaagc accgcgtgca ggatcccgag aatcccgatg cccatcatga cccaactggt
    39241 gacgtccagg tattcccaga aaatcaagga agacagcaaa ataaccgctc ccactcccag
    39301 ttcgccccag cgaacctcac gacggaccgt catccccaga attcggtcgt aagggaggag
    39361 aatcacccgg gtgtggtccc gggaatccgc cgtgggaggc tggtctggga cgatcttgga
    39421 ctggacgaag aacaaccggc ggtcggtggc gaaaacgctg caggcctcct gctccggttc
    39481 cttcagtaga gactgggtcc acttgaagtg agcctggatg gagtggtgtt ctctttcgat
    39541 gggttcatcc ttggccatcc tttttcggat gacggcccgg tagagctcga tggtgaaaaa
    39601 agtgagcagc ttctgaaaac ccttctcgcg aaggtcccct cgctttcgct tggcgtacag
    39661 ttcctggatt tccttgcgca aatcttccgg ttccggcggc atgacgactc gttcaaccct
    39721 cttatgacta aacagcatcc tcattcccgg gggaacggac gagctcgagg gcccgacctt
    39781 cctccgagtc ttcccgactc gggcgttcat ttcgaagggt ggaggcacga acacttctct
    39841 gctcttccac caagggagac gccgggttga ggggagaaag cgtctccggt cccggcgcca
    39901 aacaaaaggc ccgaccaaga tcttatcagc tttcgcaccg tctgtggacc cgaatgttcc
    39961 cgcaaatgga gagccgacgt cttgtggcgg gcctctttct cgtgacgaca aaccgtccgg
    40021 tatccatggt atccttctcc tccctctccc ggacggaaac gggaacctcc tgccgaatca
    40081 cggcggcagg aagaaaaggg acggagacgg acgcccggaa ataccggacg ggctttcaaa
    40141 aatgccccgg aggccccggc aaaagccggg gccgcagggt ccttccattg ctaatcgtct
    40201 tcgtacggat acgatcccgg tgacacgtgg gatgcgtggt acccgggcag cagggtcagg
    40261 gtcacggcta ccaggagcac aaaaaccacc acgaaggcgt tccaggggtc ccactccgcg
    40321 atggtggact cgtagacctg ccaaaggaga agcccgtaga gcacgatgtt tgccaagatc
    40381 tgcatcaacc cacctcctta tctagaacat gtgctcttta agccagaaca catagatcaa
    40441 tcccgcagca ctcagagaaa tggccaaaag caagacccag tagccgatca tgttgtcgat
    40501 gaaccatctc atgacatctg cctccttcca aacgagagat ctcggagaaa actctgcatc
    40561 aatccttcac caccagggac ggaatccagt tgggaaccgg ttttccggct ttgtgccgtg
    40621 ccgcaaagat caccaggctc atgatgaaaa gacacaggaa gatcatttcc cccagggtga
    40681 acaccacggc ggcaaaggac aggatctcgt actggcctcc cgtcatgaga tagcccagcc
    40741 gcatgaaggc gcccaccgtg gccaggatca gcgagtagct caggatgtac cggatggccg
    40801 ggccgcgcac gaaggacgtg gccagggcgc cgaactgagc cccgatcatg gccccggaga
    40861 tcatgaagag gaccgccatc atgtccacgt tgccgctcat ggattgccgc agggctccgt
    40921 agccccccga gaccacgatc tcgagaaggt ccgtccccac ggcgatgtgg gtggtggcgc
    40981 cgatgatgta gaccagggcg ggcacgcgga tgaaaccgcc gcccacgccg aagaacccgg
    41041 ccagtagccc cgtgcagatc cccaccacga cgatcaccca catggagatg acgatgcggg
    41101 ccacgtggca gcgaacgatg gggaacaagg ggatcttctg gaaatactcg ggcagccggg
    41161 tcgccttgtg ctcacgtccc aaatgggcgc cgctcttctc tcgttcctcg atctgcttgt
    41221 gggccttgcg ggtttcgatc tgggtgtaaa taaaaagtga cagcatgatg accaccgtag
    41281 ctgacagtag ggcgatgccc gagacccccg ccttcttggt catgttcagg agccgcaccc
    41341 cccattccac tcccatcatc gtgccgccca ccatggcgag gcccagggtc cagtcgatgt
    41401 tgcccaactg ccggtggcga atggtggcca ctaccgattt tccggcaatg tgggtgacat
    41461 cgattcccga agccatgtag ccgggaaatc cgaatacgat gagggcgggg gtcaccatgt
    41521 agccgccacc caccccgatg aagccgccga cgatgccgac ggtgagtccg gtcaaaacaa
    41581 gcaagaatac gggcgattga atgccagcaa ttgcaaaatc gaacatggtt gcctcctccc
    41641 tagtgcccac cctcgtagtg tttgatcttc ttcaccttca tgcccagcat gcccgtgaag
    41701 ccttcggcca tcgttcccag gcagaacccc agcgccagga tcaccgcgac gttccacacc
    41761 cccgaccagg gattggtctg gacgcccttg tgcttgtagg tgtcccggta cttgaagccc
    41821 ttggcctcct tgatctggct tggcaccatg accttgaggg tgtttccttc cagggcaacc
    41881 cgctttcctt cctcgacgtc cttcttggag atcaccccgt tggcgttctt gggcaaccgg
    41941 tctcccttct tcaccggtac ggcctgtagc cccttgatct ccgccgcctg gacgatttcc
    42001 agcttcatcg gcttgtcttt tgtggcatcc ttgaacgtat tcacatccac ggcatagccg
    42061 gtggtgctgg tctcgtaata gtaccagctg atcatctccc ctgaataata ctcgtaacaa
    42121 atatagaaga ctaatgccaa agacaggacc gtccagacaa tgaacttgat ggtctctctc
    42181 cttctataat catccatgca accaacctcc tttcgtcaac tcattcccac tcgatgaact
    42241 ttcactcgaa catactgaaa acggatgact cggtctgtac tgcgtcggca ttggagctgt
    42301 tccgctgcgt ccctcctgtt gtcggtgtcc gattcctgcc gtacttccgc cctctgcccg
    42361 gtgggcagcc acgatttcct gacgccgtca cctcctttcc gatggttgtg aattgtgtgc
    42421 ggtgcgctca ttattcccct tatgggcatg cccccgccgg ggcacattgc ctcactcgcg
    42481 aactacggga tggattccgg gtatcggacg ttgcggtgcg ccacgaagaa aggtaggcgg
    42541 tttcctggtg cggagggttc catgggcgcg tgtaccggag cttcaacaag tcgatcccca
    42601 tcaccattca aaattccctc aacgaacagg atcctcacgc cccattcttt attcaagact
    42661 gatgccaaag cgacccgttt agagggaagg ccccgggcac aaacgggggc cccaccgggg
    42721 tgaaacgggc ccggaacggc cccgggcaaa gattcggccc tcgaaggcct cggccgtggg
    42781 gaaagggatc gtccctccgc tcctccggtc cccgggtgcc cctcgagacc cgggaaaaca
    42841 agatgggaaa accatccgcc ttgggcatcc tctgcgcggt caactgtttg tgtgaaaaga
    42901 atgggtaatg gaacgaagac tccagttcat ttttacccta acagaggtcc cgaagaactg
    42961 tcaaagggaa tttgacgggc acaaacatcg tttgccccgg cgaaccgaat cgcacaaccc
    43021 ccggggtggg tcaggaaaga tagagggatc gtaattcgac ggtcaagaaa acatgagaaa
    43081 tatccgatag atttgacgat tcacaaaatg gcgtgggtca taagggtaca gtagcgggtc
    43141 aggatgaccc gaataaaaga aagggaatcg gggaatcatg cacaaaaggc ccgctccaat
    43201 tgataatcct cctccagaaa ggcccgggca atttcccgca cgctcttttc actgtgcatg
    43261 agcatgtcca gctgccgggt aaagagcagc aatctcccca gcaggtccag cttttcctgg
    43321 gtgaggccca ggtcgtattt ctggtaacgg gcgtccaccc gatctcccat gacttccacc
    43381 gcaaaaccga tggtttccag gatgagggca atggaacggg ccctgcggtt tcgcctcacg
    43441 ccgtccgccg ctccgccctt gaacgaaaag gtgatgtagt tcttggagat cacctcgtcg
    43501 cagaaggcgt ccaggatgcc gtaatgatac cccacccggg agctgaaatt gaggtagcgg
    43561 tccgatacga tggcgtagct gcgatctccg aatcggtccg ccgtgtgggg ggtcgagagc
    43621 atctgttcgc tcatgaccga gagaaagccg ccgagttcca cgggacgggg acgctggtag
    43681 cgaactcctt catggagcat tccccgcagg agggtcttga acggcacgga gaccacctcc
    43741 tccaccgaca cctttcttgg gtggggcttt cggggagcca ggccgcctcc caggtcgatg
    43801 acgcacaaat ccagggggat gggcgcctgc aaaagcaccc cgcattgtcc ccgttccgaa
    43861 accacgtcgc tgatctggaa catctccttg taggagagtt catggacgta gcgcatgatg
    43921 tcatggagcg attcacagtg caggggggag aactcgggcg ccttcgggtc gaagagatgg
    43981 aggggcacga tccattgggc cacggatcgg agggtctcgt acacgggagt ccgtttcatc
    44041 cttggctctc ggtcttttcg caattggagc agttccggca cgcgaccttc gtagaccttc
    44101 ccgtagaagg cgtccacggt gatttccgtt ccgtggggaa tgcgtcgtgt cgcactccgg
    44161 gttcccagga gggtcgggac cccgaactcc ctggccaggg aggccatgtg cccggtaatg
    44221 ctgcccgtgt ccgtaaggat ggcctgggcc ttgggcatga ccaccacgaa cttgggagag
    44281 gaattggcgg cgaccaagat cgccccgtcg ggaaaattgc ccaggtcctc ctcggagacc
    44341 acgtgaaagg cagggccgaa cccgaccccg ggacaggccg cctgtccgcc ttccaggagc
    44401 aggggatacc ctgccggttc ggggacctgc acggtctcgc ccccctggga gggggcctcg
    44461 aggcgcaggg gcctggtctg cagaatcaag aggcgtcctt cggggtccac ggcccactcg
    44521 atgtcctggg caatcccgta atgggcctcc agtttcacgg cgtaatccgc cagggtcttg
    44581 acttgctcgg gggtgaggca gggggcctgt tggcgatcgt cgggaacggg aacctgcatc
    44641 aacccgcctt ccgggttgcg aaccagctgc acgggcttat gggagatgtc ggattccagc
    44701 agggtatagt gcccgtcttt ggccacggta taacggtccg gggtgatcac tccgtccacg
    44761 gcatagggcc ccagtcccca gaccgcgctg atgaggacgt ggtcctggaa ccggttgaag
    44821 gggtgatggg agtacatgac cccgctgcac acggaatcca ccatctggag acaggccacg
    44881 ctcatggcga tgtcctcgtc ccggattccc aggttgaatc ggtaggcgat ggcccggggg
    44941 gtgaagaggc tcgccacgat gtagttgtac gtctggatca agcgggaccg ggggacattg
    45001 agcaccgaca ggtactgacc ggcataggac agctcgctgt cttccccgat ggcgctgctt
    45061 ctcagggaca cgtcccggca aaagccgccc gtcgtgcggc tctcgatccg ctccatcatc
    45121 cggtcgtagg cttccaggac ggcttccttc aattcctcgg ggaccgaggc cgaaatgatg
    45181 agcctctgga tttcttcgct ggcctcgttg acggactcgg gattgcgggg atccgtcagc
    45241 atcttgatct tattgatctc gtccacgaga tcgttggccc ggaggaggag ttcgtaggcc
    45301 cgggtggtga tggcaaatcc ttcggggatg ggcagatgag cccggttgag cacctccccc
    45361 aggttcgagt tcttgccccc cacccaatcc accatctccc gggtaatctt ggaatagggc
    45421 agaaccagct cctgaaccgg gttctctttc ctcttttcca gctgatcctt aatgaggcgg
    45481 ttgagggaat cgagcttttc gtggagaagc aggtaccggt tcgaggaaag accgttgaga
    45541 ccctggatca tgcgcagggt gcagaacacg gcccgggcgg actgggagcg gacgtacgac
    45601 attccgaaga gctgttcgcc ccggagcttc tgttcaatat ccgtgatgat tttcgaaagc
    45661 tgggtgttgg catccagcag ctctttaaag tgctggtatt tcagtcggaa gatgtccgac
    45721 aggtcttcgc ggggtcgggt gtgctttcga cccacccatc ggaagggatt gagtttctcc
    45781 aacatacggg aggtcccttt gttttgagtc catgagcatc cacgggggaa tcccggccgc
    45841 cctgcatgaa cttgggatgg ttttccccgc aggcatattc ctgagtggag cgattccgag
    45901 agcggcagcc cgccctgccc acgacggcgg gcatccggag ggtgcgaacg ctctcgtcgg
    45961 gtccccgctc cccgggaatg gacgaaacgg gattcgtccc ggcaaaatca ctcaatcgta
    46021 ccatattatc tatagcatcc tccgttccaa tggaacgaca tatttccacc gtggggtgcg
    46081 ggaagaggga ggtcctccgg cggggggccg gggccgacgg gggcgagggc ccgcagggtc
    46141 ccggaacggc cgggacaaaa gggttgcatc ttggtcgttt gtgcgttacc agtcaggaaa
    46201 agacccgaaa tggacgattc gtcccatcct tcataacaat gcaaccgcct cgaaaggagg
    46261 atgtccatgg tttccgatgc aagatccggc gttgcggtga tcctggttct gctgttgttg
    46321 tcttcctgcg ccgagttcac ctttgccccc ctggccaagc acaaggcccc acctgccggc
    46381 gactttcggc tgcttggcgt ccaggtcccg gaatccgtgc aggaagacat tccgtacgac
    46441 atcacgggga cctttcaggc gacgggggaa gtcaaggtcc tacgggcctg ttttcgatgg
    46501 ttttccgacc gagcgcagat gagctctccc cctctccatt gcttcaccta cgaagtccag
    46561 accaatcagc ccattgggtc cgtgtgctcc cgttgggtcg ccgaagggcc ccatgccatc
    46621 atgtctcccc tgacctgcgt tcgggtggaa gacgttcgtt acggcacacc gggcaggttt
    46681 gcggtgaagc tccggtcgag caatctgaag ctcgtctaca gccagatcga gtgttacctg
    46741 gaatatcttc aggatggagt gaccaaggag agcaacaaga tcaccgcccg tatccgggtg
    46801 ggggaatgag gcgggatggg aaaggtttgg aagaggtcgt gcggtggcct gcagctgtcg
    46861 gaaggaatgg tggtgccggg aaagatcggt tccggtgggg ggccgctccg agggacccgg
    46921 cgggccggcg ccgccccgag ggaggcacga ccccgccgag agggtctcgt cctcgaagcc
    46981 cgggggacac ggatcttctc cggatgactc cccgggatgc cgtttaagga atatcctcct
    47041 cgggacccga tcccaagacc gcctgcccaa tcatcgacgt gctctcgcac cgggaggcta
    47101 tgggcgactc cataccgcgg ccagggcctt ggcgggcttg tcgctgatgc agcgaaagct
    47161 gtggctcaaa tcggactcga aataaatgct gtccccggca cccaggattt ccacccggtc
    47221 caccgttcga aattccaccg tcccttccat gatgtacatg aattcttccc cgtcatgact
    47281 catgaaaatc atttcgtccg tctgctgggg cggaaattcg ataaggaacg gttcgatgtt
    47341 cttatttgtc ttcttggttt ccagggcttc gtaaatatag ttcacttccc cctgggactg
    47401 atgaggcctg cgttccaccc ggatcctctc cccctgccga gtaatggaaa ccttctcgtt
    47461 gccgacctcg tcctggaaaa aataggccat gccggcattc agggccctgg caagcttcaa
    47521 aagggtcgcc acggggggaa ccacatgatc gttttcgatc tgagaaagaa agggtttgga
    47581 cagaccggtc ctggcggcca gatcttggag tgtgaaccga ttcttttgcc gcaattcccg
    47641 gaccttgttg ccgatcttca aggcccgtac ggccaggctg atatccttgt ctttctcttt
    47701 ttcttttgtc tgtgtcatgt ctaaatcctc aaatgctgtg atcccgggcc agtctcgact
    47761 gaaaccctcc gcatggacac cgaggaagat ggaactcaaa tccattgagg ctcagagcag
    47821 taagcccccc cgtgatggtc tccttcaaca aagtcagacc gggaggagag ggagaaagcg
    47881 aaccggaaga gcagcacgcg aggtcctgac catgtccggc aaccgactgg gttagacaag
    47941 tataaactat gtttggtatc atgaaaaaca aaatccactg ctccggttcg aattttttcc
    48001 tcggtttcca cccttgcccg ggggaactcg aattccccgg gcgaaccgtc cctgggggca
    48061 cagggaccgg gtcggcgcaa acccttaccg gaaaaggaat tccacgaaat acgactccac
    48121 ccgaacagtc gtctcctggg gacggacccc ggggaccctc gatgcgctcc aggacggacg
    48181 ctttcctctc caatgggaag aacgggtcga tcaggtttgg tgacggcaaa acggagtcat
    48241 atccccgggc agtcaacatt cctgggagga gtccggtcgc ttcattccaa tcccggaggc
    48301 ttccccggtt tcaagtcgtt ccggaaacgt ttgaagtgcg taaattccgc cgcctcgcgc
    48361 ctccggggag ccggcgggac ccgggagggg cagcccgggg gaacaagcgc caccggctgg
    48421 tgttccgggg gaatctcgaa gattcgccct gcctcctcgt ggtcgaagag tcccacgatc
    48481 acggttccca ggcccgggtc gcgagcggcc gggcagaggt tttgggtggc gattcccagg
    48541 tcgaaaagca gccggtcccc aaaccgggtc gtgacccggc catggtagtg cccggcacgg
    48601 ccgagtttgc cgcaaaccac gagaacgacg ggggccgaca cgacggactt ggcggcggca
    48661 ttgccgggtc ctaccgtctc ccggaggcgc ttctcgagtc cggggtcccg gaccgcgatg
    48721 atttcccggc atcgaggatt cgcccgggaa ggagaccagc gcacggcctc gaagacctgg
    48781 gacaggaggt cgtcgggcac gtccctgtcc tggaaactgc ggatgctgcg cctctctcga
    48841 atcccgcgca tcaggtcgga cagggttcat cctccctggc cgattcccgt ctttccccct
    48901 ttgtcatcct catcatgttt ccatgttctc ggggaagaag ctgaagccgg atggtcttcg
    48961 cgccgtggcg gggaagagtt ctccccaaaa cgagcgaact cttgccgttg cacggcacca
    49021 aagcccgggc atcagggcgg cccggaaacc gcccgcccgg cccaaacggg tacaggcggc
    49081 cctcgggggc cggttcatcc ccgcgccggg agggtgccgt ccgatgggac atggccggca
    49141 agggccgccc ggttgatgtt ctcaactgca aacccgcccc ggacaggacc ccacggagac
    49201 gggggtccca tatacaccgg aagcgggtct ttactccaga aaaaaccggg ttccccggaa
    49261 atcgatccga ttccggcttt ctgaagcaag ggtcctggcg tccgggtcgt gggttgcggg
    49321 gatgccgggc tcccggcggc ctgtctgccg gagagctatt ccggcggccc cggttccaaa
    49381 agcttttcga ggtccttcca ggtccatggg gtccctgcag ggggggaccc ttggggaggc
    49441 aggggccagc cctcgggaat gggggattca aaaagcaggg gctcccggcg ggtcgggtgc
    49501 tccacgagga ggcgccaggc caggagtgcg atttgtttgc cgggaagggg gtcccgggct
    49561 ccgtaccgga ggtctcccag aaccgggcac cccaggtgag agaattggac ccggatctgg
    49621 tggcgtcttc cggtttccgg acgcacttcc accaggctga cgggaccgga tgagcacaga
    49681 aggcggtagg agagacgggc ttcttgtccc tccccggcct ggggggcgac ccggctggat
    49741 cgatctcccc ggcgctcgac tcgatggacc agggtctctc gatcccgggg cggggtccct
    49801 tccaggacgg ccaggtagaa tttctcgacc cggcgggcac ggaactgggc gctcagccgg
    49861 cttgcggcct tggacgtgcg ggcaaagacc atcaccccgg ccacgggacg gtccaggcga
    49921 tgaaccattc ccaggaacac tgcgcccggt ttgcgatacc gctctttgat ccacgatttc
    49981 cccaggtcga ggaggcaaac gtcgccggtt tgatctgctt gtaccaggag ccccgcgggc
    50041 ttataaagag cgagcagatg gttgtcctcg tatagaagag gccatcgagg atggtggaaa
    50101 tagaggtcgt gcatggggtt gactcccggg gatccgttgt cgggcctgca accccggaat
    50161 ccctgacgga ttggtccctg cctcgaggcc ggggaagacg gcaggccgtc cgattcccct
    50221 gtggctccag ggcagcgaga ggaaaggctt cgtggaacgt ccaaggcccc gcccaaattc
    50281 tggatctccc ggtccaaaga ccatgggagc gctcctgcgg gaaagcggaa tccgcctccc
    50341 cccccgcgga ctcgatcagc tgtggagcta tcatcagctc ctgcgccagt ataaccctga
    50401 tctaaatctc agtcgaatca gcaatttcac caacatggtc caaaaactgt acgtggactc
    50461 gatcctgccg ggccaaatcc tggagcttcc gtctcctctc ctggatttgg gtaccgggcc
    50521 gggcatgccg gggattccct tgaagatcgc ctttccccgc ctggagatcc tgctggccga
    50581 aagtcgccag ggtcgagcgg ccttcctcga acgggtcctg caggagttga agctttcggg
    50641 gatcgaggtc gttcccatgg ggatcggtcc ccggtacgag cggcccgtca aaggggtgat
    50701 cacacgggcg gtggagtccg tcgaggagac gcttcgtcga gttcgggggt gcctggatca
    50761 gcatgggctg gtcatcttca tgaagggccc cgggtgtgac gccgaggttg acaccgccgt
    50821 ggcccggttt tcccgggaat accgtctggt gacggaccac gcctacggca tcgccggcac
    50881 gccccacaag cgtcgactgg tggtgtttca gcgtttggat gagcccgaga gggtgaggaa
    50941 ggtgctggcc atggaacatc gtgtggtaag aacaatcgag agcgaaggga accgggaatt
    51001 caagagcttg agaaagcttc tggggagccg gggggtcaag aaggagcagc gggccttggt
    51061 gtcggggccc cgccaggtgg cggaagtgat cgagtcccat ccccaagcct gcgacgcctg
    51121 ggtcagcgaa ctggagggtc ctgcgccgcc ttcgggagtc ccccgtccag tctcctgggt
    51181 ccgactgtct ccggagcttt ttagaatcct ggatctcttc ggcaccggtt ctcccctcct
    51241 ggtggtgaag attccgccca ttcccccctg ggagccgtcc cgggggctcc ccgggggctg
    51301 cagcctgctg cttccgtttc aggaccccga gaatgtcggc agcgccgtcc ggtcggccgc
    51361 ggccctaggg gtgtctcaga taatcctcct ggaggagtgc gctcatccgt ttcacccaaa
    51421 ggccgtccga gcttcgggag gcaccgtgtt tgccgtcaaa ttcctccggg gtccttccct
    51481 ggaaaccctt ccggatcttc ccggcgtgat tcccctggat gctcgaggca agaacatcga
    51541 ggagatcgaa ttccccccgg actttgctct tttgcccggg atcgagggac cgggccttcc
    51601 gggcccctgg cgaagccggt ccgccgccat tcccatccgt tcgggaatcg attccctcaa
    51661 cgcggccgcg gccgtcgcca ttgctttgta cgtctggtct cgcaggaagg aaacgaattg
    51721 aagccctcag ggagacttgg gggtcaggaa gacggtaacc cgccggttca tttgccttcc
    51781 ggtttccgtg gcgttggaaa caaggggcat ggattcgccc aatccttcca cggagatcct
    51841 ttccgcgtgg attcccgatt ccaccaggac ctggcgcacc gcaacggcac gacgttcgga
    51901 aaggccctga ttgtatcgtt cggacccgga gctgtcggtg tggccgcaaa tcttgacctg
    51961 ggtttccggg tagcgtttca ggatctgcgc cagttggacg atttcaacgg aggtccccgg
    52021 tttgagcagg gtcgagttca cgtcgaagaa cacgtcggag aggaaggtta cggccaggca
    52081 attttctgct ctctcgatgg tggcaaagtc cttttgagag aagctcgcac ggaggtcgcg
    52141 ttcctggcga tccaggtacc cgaccagggc ggaaggcggg acggggggag cggcctgggg
    52201 ggaatccttg gccccgggac tcttcccgga ggcatccggt ttccccggct gcaaagaccc
    52261 ggcacaagag gccaggaacc acaatgcgat cgccgaaagc aaaaggacct ttttttgcgc
    52321 catttctctt ctcccggaat cactaccggt tctgccgggc ctcggggagg atggtaagga
    52381 cccccgaacc ccggagccgg tcctgtttga gcctgaggag cgcctcgttg gctttggcca
    52441 ggggaaaaac ctccgtccgg ggccggatgg gaattcgtgc cgcgacctcc aggagatcga
    52501 gcccgtcctg ccgggtgttg gcggtgacgc tgcggaggtt tttttcgtag aagagatgct
    52561 cctcgtatcc cagggaggga acgtccgtca tgtagatccc ggccagggcc agggtgcctc
    52621 ccttcttcaa ctgcttcaac gctggtggga cgaggtcccc ggcgggggca aacaggatgg
    52681 cgctgtcggc gggggtgggc atctgttcgg ctcgagaccc cgcccaggcg gctccgagac
    52741 ggagagccag ctcccggtgt cccgcattgc gcgtgcacac atacaccgta catccccagt
    52801 ggagggcgat ctggatcacg atatgagccg aggacccgaa accatacagg gccagggttt
    52861 gtccggggcg gcattcgctt cgccgaaggc tccggtaccc gatgatcccc gcacaaagca
    52921 gtggagcggc ctcgagatcg ctgaagaccg aagggatcgg gtaggcaaaa tcttccggca
    52981 ccacgaccag ttccgcgtac cctccgggcg catggtagcc ggtgaagacg gaagattcgc
    53041 aaaggttttc ccggtcgctg cgacaaaagg agcattggcc gcaggcatgg tgaagccacg
    53101 cgattccaac ccggtctcct acgcggaacc gggacgtgcc cggacccggt tggtcgacgg
    53161 tgcccacaat ctggtgcccc gggatgatcg ggcggtccag gggcggcagt tccccttcta
    53221 taacgtggag atccgttctg caggtcccgc aggtatggac gcgaacccgg atctcccccg
    53281 gaccggcaac ggggtccggg acttcccgtc gttcaagggg agcggtttca atgggggcgc
    53341 acgatttcag taccattgcg ttcatgggag aaccttccct cgatggtgat aggcagttga
    53401 tttgtattgt gttcttgccc ataatgcaat agtagatctt tccatggcga atggagactg
    53461 atgttgctgg gtcggaaatc aaccaataag gaacccgccg ctgccggctt gtttcccctt
    53521 cgttttctcg tttggggatc cgccttttgc ctttttgtcc ccctggtttt tctttttgcc
    53581 atccatctgc ccgccctcca ggagcagctt atcggcaagt tgattcggga agtcgaacgg
    53641 gccacccctc ttcaggtgcg actgggttct taccgactcc ggcccttttc ccggttggag
    53701 ctcttgaacc tgaacatctc cgcttcgggc aaggaactgc tggtttgtga ccaggcaagc
    53761 ctggtgtacc gaatttccac cacctggccc tatgtgcatg ccacggaact cctgctggag
    53821 aaggccgtat tgaatctcga acgggatacc cagggcaact ggctcctgcc tcgaaaccgg
    53881 aacgccgaca agcatttccc gggccgggtg cacccggact gggtccgaat tcctctgccc
    53941 caggtccgaa tcgtttccgg cacggtcacg gctcaccagg agggaaagcc cgttctcacc
    54001 ctgcgcaacg taaacgggac tctgtccttt cgggtggtgg aggagaagga aggaccccga
    54061 ctcatcatcg agatgggggg gctgtcccag gaaaggagtg cccgagagcc ccagggaggg
    54121 gattcggcgg gatcgaaggg actcaagcct tccaacctgt cgtacctgac tattgacgga
    54181 tcgagtcagg tggcgggccg ggggacggtg gacctgtttc ccgacggcga ggtggacgtg
    54241 gaagtctggc tggatccagt ctcccggtcc ctttttcccg ccctggccga gcaggtgggg
    54301 gagctcaagg agttgaccgg ttatgtgcgg gcccgccgcc aagggggggc ctggactgtt
    54361 gaccacgatt ggaataccaa cctcgggact gcccggggaa gggcaacggt tgggtgggaa
    54421 aatgcggcgg aaggaacatc gggaacaaaa acggtccgct ggtcgggccg attcgagaac
    54481 cttgccgttc ccccggttgc gtcccccggt cccggcggtc tttttggcga catcgaggtc
    54541 gaggcccggg gtcgcgatct ttcggatatg gaaattcggg tgaaggggca cctggatccc
    54601 tcgaactggg gggaccagcg gattcaaacc ggaatcctct ccggttccta tgccggggga
    54661 gtggcttccc tggaggccgt ggaagttcgg agttccctgg ggaacttttc cgcccggctt
    54721 tgggccgacg ttcgcggagc atgggatccg caccacgcag gaaaggccgg ggttgatctt
    54781 caggtcgaac ggggggccct ggacaaggta ctccccgggg ccaagcagcg ggccggaggg
    54841 agcgtgaagg ccgaaggccg gtactccccg ggcaggttcc gggactggaa ggattggcag
    54901 gcaagggtcg aggggactct gaacctgccc gatttctttt ccctcaaagc ttcgggaacc
    54961 tatgaaaacg ccctgctccg gttcgactat gaactggaag cccccgaaat cgggaaggtg
    55021 tccgcgttgg ttccgtcctg ggagggcaag gggcggattt cgtcccgggg gagctttcgg
    55081 ggaacctggc cggacctggt gtgggaaggt tccgtgagtt ccccgcgcct ggaatatggt
    55141 cccctggttg cggaccaggt ggccgtgaac gggaagggga aggtcctcgg cagggacgga
    55201 caccggaagg ccgccctcaa agcgaggggc tggtccctgc atggaaacaa gatgggggcc
    55261 gttcaagcgg acctcgaaca gcaggacgga tcctgccggt ttgaaatcaa gggggagcag
    55321 ctctggcaag gcaccagcgc aaggctggcg gggttgctcg aaaacctttg gctccccacc
    55381 cggacccttg tcctcaaacc gagccgggtg aactggaaag atcaagcggg gtccgtggaa
    55441 ggcaggatcg agttttccgg cgaccgcatc cagatccact ccctcaccat ccagcagaac
    55501 cgccagaaga tacaacttac cggggctttt gcgccggatg cccgggcgga cctgaccctc
    55561 aacctggatt ccgtacaggt ggcgccctgg ttgcggtttc tggacaagaa ccccgtcgag
    55621 gccgggagcc tcgggggaca aatccggctc acgggacggg tggaacagcc cgaggcgacg
    55681 ctgaatctgc agatcaccaa cgtgaccttg aaccctgagg tctttcgaga caagtcttcc
    55741 ggggaggcgt tgaaccgggg cacaggccgc gatacccgag gccagggctc ccaaaccgtt
    55801 gaaaagatcc tggtgacggg aaatttctca cgggacgcgc tccaggtgaa ggcggacctg
    55861 caggcggcgg cgttctcggc ccccgccgtg gtgaccgcaa ggattcccct tcgtttcagc
    55921 ctcgatcctc cccgcctgga tatcgccaaa acggacgagt ggagatcgac cctgagggtg
    55981 acgggattct ccgctgaccg gatcctaccg tacgtggagg acctcagcaa ggtggggggc
    56041 cgcatcgacg gggaggtcga ggggggtggg agctggagtc agccctttct caaagggtcc
    56101 ggcaccctcc aagacggcga ctttacggta aaggcatggc ctcatcccgt cgagaagctt
    56161 cggggagact ggagtctgga cgagaaattc atccacatcc ggagcgccga ggggcagctc
    56221 ctgggcggcc gggtcgaggt caagggtcga gtgcgctttc cgtcttttcg ggtcgcagag
    56281 ctcgaggcga ccggggaaaa cctcggggtg acggagctct acgggatccg cgggcgggtt
    56341 tcggggaagg cccgcctgtc cgagctgtcc gaaaacccgg aactgacggc ggacctgcgg
    56401 ttttccaagg ccgagatgca gctgggggac ctggaaacgg acattgcccg gaacatccag
    56461 attgtggggg gagacgaaca ggaaaccctg gtggaggtac agtcccggag ccggaagaaa
    56521 cccaactact tccaccgcat gaagatggag ctgcacctgg ggctccccga ctcgggaagc
    56581 tgggtccggg gcaaaggcct cgatgccgag atcaacgggt ccatgaccat cgagaagggc
    56641 ccccatggac ccgtaaggct cctgggcagc ttctacgccc tgaggggcac ctacaatttc
    56701 cagggaaacc agttgaaggt ggtggaggga gagctcattt tccgggggag tcccgagccc
    56761 gatcccgtcc tcaagatcat ctgccagaaa caggtgaagg acgtgaccgt ccaggtccag
    56821 gtgacgggac ccgtcagtca gccgaaactg gccctgagca gcgtgccctc catgaaccag
    56881 gtggacgtgc tatcctacct tctattcggg catcctgccg gggagctcaa taccaagcaa
    56941 caatttcagc tccaggaccg cgccgcgtct tggatcggtt ctcagacctc tcatgtgctc
    57001 aaggatgttt tcggaaacaa cccatggact cccgataccc tccactatcg cagctccgac
    57061 gaccgtaagg agggggccgt ggtggaaatc ggcaagtatg tgacccccga tctttatgtt
    57121 acctacggga agggaatccg gggagaaacg gaaaaccagg tccaggtcga gtatcgcctg
    57181 aatcgtcacc tgtccgttca aacccaggtc gggggagccg aacagagcgg agtggatgtg
    57241 ttctggcgct acgattttgg gaagtgaaag gagaaatcct catgaagatg caggatgtgc
    57301 gtgtcatggc aaaatccatg ggaatcaagt gctttggcaa gaccaaggcg aatctcatcc
    57361 gggagattca acgaacggag gggaattttg actgttacgg atcggctcgg gagcattgcg
    57421 accaggaagc ctgcctgttc cggagttcat gcctggacga gaacaaggaa aaacccaaga
    57481 agtagaccat caaaggaccc ggaatgactc aaagttccca gacagtacag gagaacgtcc
    57541 tgctaacctt acagaacagc aggaagcccg aacaggtggt gtccgtcacc atggacccga
    57601 cccgcaagcg cctgcagacc cgcggactga aagacttgtt cggcgtcgac gagatccgtt
    57661 tcgaaacccg cgacctggtg gagaacctcc aggagatcgc ccaggtcatt tcctttttgt
    57721 tcgagaccat ctcccgggcc gaagacctcc atttgcccta cggctaccag gacgagttcg
    57781 aatttcaggg aattcgctac acgttataca aggaagaggg agtgagggtc ctgcggcaca
    57841 aagaacggtg accggcccgg ggggaaagcc gcttcgcagg catccccttc acttcttcca
    57901 ggtctgtgga agattgtgcg tcgaagctcg gaatttcatg tcgtgttcca tggggccgcg
    57961 gtcggcgttg cggacaagag accctcacgg gtcgggagga gagacacccc gatgaaacgc
    58021 aggtttcgtg ttttgttttg gctcttcgcc ctggccgcct ttgccgggac cctttcccat
    58081 tggcccgccc gagaggacgg cgggagcctg tcgggcagcg tcccgggcag tctcccggat
    58141 ctttccccca ccgaggtcat ggctcagacg gcaacccccg gtccctcctt cgcggaagtg
    58201 gcggcgattt tcaagaaata tcactgcacc gtctgtcatg gaggcgcgag tccgtcgaga
    58261 ggattgtccc tggacaaccg ggaaggagtg ctgaagggtg gcaagaaagg gccggtggtg
    58321 attcccggcg agccgggaaa aagcgagctc ctgaaacgta tccggggagt cagcgaaccc
    58381 cgaatgccct atgacgggcc gccctggctc acggaagcgg aaggggccgt catcgagggg
    58441 tggattgcct ccggagcccc ggccaacggg tagaacccgc cctaaaatcg gtacatgtgg
    58501 agaatcaggt cggcccgacg caccatgccg atcattttcc cgtcctcgat gaccggcagc
    58561 aggtagctgt ccacttcggt catgaggcgc agggattccc ggatgttttg atccgggtgc
    58621 agcgtaggga tgcgggtctt catgtacgcc cgcaccggcg ccttcatctg ggcgggggtc
    58681 cggggtttgc aacattcgac ctcggagacg atcccccgga attccccatt ctcgaccacg
    58741 gggatcgccc gcaagcggct ttgagccagt agcgttcgcg ctgcctccat tccgctgttc
    58801 gaggagagaa agggctcggc cctggacatg atgtcccgta ccgacaatcg cggtcggcgg
    58861 atctcggccc ggatgagggt ttccagttcc cggtagagga cgtccgggtc cttcccgtgg
    58921 accacggcgc tgcccgcccc gaagtgacct ccgcccccca accgacgcat gacggcccct
    58981 acattgacgt cgctggagcc tccccggccg atgattatgg accgctgctg atccgtggcg
    59041 aataccgcga aggccacgtc caggtgcttg atgtcccgga acttggcgac cacgggagcc
    59101 agcaacccgc ctccgccgtg aattctcagg agggacgtcc ctatgtggta cccggattcc
    59161 cggtagagcg ttgcaccgtt gaggagctga gtgagcacct ccgtctggtt gtcgtcgaag
    59221 gacgaggcga gataggcccc cgcgacgttc aggtccgccc cgttttcgag gagaaaaccg
    59281 gccgtgtagg cgtcctcggg agtgaccgac ggataggtga gattgcccgt gtcctcgtac
    59341 aggcccaaca gaaaaagggt ggcgtgcatg ggggtgaagg cgcattcccc ctcgcgcatc
    59401 cggcggagca ggagggtcac cgccgccccc gtcggctccc ggcagctcca ggaagcatga
    59461 acgttgcccc cctccatgtg atggtcccaa agggagatct ccaggtccgt gcggtcttgg
    59521 aggtccttga gcgggtccaa acggctccaa tggttcgtgt ccacgatgac cagggaggtg
    59581 acgccggacg ggtcgatctc ccgcacggag cacagccgga aaaggtcttt gtgaatggcc
    59641 aggaactcct gaacgttggg cagcagccgg gtgggaagcg ccccgatggc ttcggggtac
    59701 agaaacgtgg cggcaaccat ggacgccagc gcatcaaagt ccgtcatcga gtgtgtggcc
    59761 acgatctgca taccggagtc ccttccttga tcccgtgtcg ccgttccgtc gaaagcgctt
    59821 tccgacccga gagatcctgt tgacatcccg cggggagagc gatagttgaa acctttgtga
    59881 tcgccattat gacggaatcc cgggtcaggt tcaacgatat cgcccacggt cgaggaggct
    59941 cacggtgcaa aagccgggcg caacatttct tctcctcttg ttgatttgcc tgctgtcgtc
    60001 ggtgctgacg gcgggggccg cacacggctc ggacgtttcg cctccgagcc accggtgttc
    60061 cggggaggag ttggactcgc tcctggctcc cgtcgccctc tacccggatt ctctcctctc
    60121 tcaattgctc atggccgcga cgtaccccct cgacgtggtg gccgagaacc agtggctgca
    60181 acaaaacccg ggtctcgagg gcaacgccct ggacggcgcc ctcgagggca aggactggga
    60241 catgagcgtg aagtccctgg ccttttttcc ccaggtgctg accatgatgg acgacaagct
    60301 ggactggacc acccgggttg gggacgcttt tgtgaaccag gaagacgacg tcctggatgc
    60361 gattcagcgg cttcgttcca aggcccgggc cgcaggcaac ctcgagacaa accccgagca
    60421 aaagatcacc tcgcaggaat ccaaaatacg gatcgagccg gtggattccc aggtcgtcta
    60481 cgtcccggtg tacaatcccg tggtcatcta cggtccctgg tggattcccg cccacccgcc
    60541 ctacgtggtg gttccgaacg ggtacgtggc ggggccgtgg ggcatcgcct ttaccgatgg
    60601 ctactttgcc gggtccgttc tctacggagg ctttgactgg tgggggggag aggtcgtcat
    60661 tcaggacaac gggttcgtga gaaagcacga caaacacgac cgtcaccacg aagtgaaccg
    60721 ggtatggcgt cacgatgcag accacgcccg tgccgcccgg gaaaacgccg ctcgccgggc
    60781 ctcgggcaaa tcggccggcc tcgaggcggg gcgggcggat cgtgcctccg gcgccgggga
    60841 tttgagccgg gattttcgcg ggtatgaccg gagttctcag aaaagcgacc tcatacgggc
    60901 gggtgcgtct accgagcacg ggagccggga acccggcgag tcgcgggcct tcgacgctcg
    60961 ggaagccggg ccggatgccc gggagtcaag cgcccgggga gcccagagcc gatggagcat
    61021 gggcttttcc gagagcaggg atttttccag gggcatgggc ggtggggcct ttggcggcgg
    61081 aggattctct cacggcggcg gaggcggccg acgccgataa ccgcccgggt cggcacggga
    61141 aaagcatcat cgcctttctc aaggccgatc atggctgctc accggaaaac cccgccggtg
    61201 ggccatgggg gaacagcatg atttatcacg tcgaggtcga ggccggggag gccggcaacc
    61261 ggagggtgga atgccgtgtg cgcaaccatg tggttcgcgc ggatcagccc cgggaatttg
    61321 gggcggacga cacggcgccg actcccccgg aactgctggc cacggccctg ggaagctgcg
    61381 tcgtttccac cgttcaattc ctggcgatgc aaaggggccg ggatgtgcgc aacattcgtg
    61441 tgacggtcga gggcgagatc gatttctcca aggccatggg cctggggcat ggagggaggg
    61501 ccgggtttcg agatctcaag atccgcatag gatttgactc ggcaatgacc cgggaggaaa
    61561 gagccgattt catgaaagcg gtgttcgatt gcggtgcatg catcgacagc cttcaggacc
    61621 ccaccccgat ctcctgggaa tggcgcagcg gggagtgagg aaaatgaggt cggtcgagga
    61681 tgcgccccca tgacgcaacg acagggatag gaccgagcat accatgaagc ttaccgtctc
    61741 cctggctcaa atgaatgtcc tccgcgccca accccgggaa aacctggcca aaggggaggc
    61801 tctcatcgcc gaggccgctc ggagggggag cgatgtcgtc tgctttccgg aaatgtggac
    61861 cacgggcttc gactgggaga gcaatgcacg cacggctcga gaccatgagc gaatcgtaga
    61921 acaggtagga aggcttgccg agaaatacgg gatctgggtc accgggtccg tgctcgcctt
    61981 gaatgaagag gggaaggttt ccaatgcctg catcctcttt ggtcccggcg gagatcgggt
    62041 gggaacctac cggaagaccc acctcttcag cctgtttcac gaggatcggc acatggcccc
    62101 gggagaatcc tcgtgcctgg cggagacc
//
LOCUS       DAOOVC010000008        59257 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_287_length_59257_cov_6.759907, whole genome shotgun sequence.
ACCESSION   DAOOVC010000008 DAOOVC010000000
VERSION     DAOOVC010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 59257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59257
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_287_length_59257_cov_6.759907"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..1844
                     /gene="hpnJ"
                     /locus_tag="PLM79_02035"
     CDS             375..1844
                     /gene="hpnJ"
                     /locus_tag="PLM79_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HOV85110.1"
                     /translation="MKKALFLSPPSFKGFDGAAGARYPARREITSYWYPTWLAQPVAM
                     IPGSRLIDAPSHGLSFESVLQSAKEFELVVISTSSPTLRSDILFVDALKARNPSVEVG
                     FVGAHVTVLPEETLKQAPNLDFVCRREYDYTCLELAQGAPYERVKGLSYRAGDGSVRH
                     NPDRDFVENLDELPSVLPVYHRDLVIENYFVGYLLHPFISFYTGRGCSARCTFCLWPQ
                     TLGGRRYRTRRPEAVVREVEEGKALFPGAREWFFDDDTFTADPARAVEISKGMKRLGL
                     TWSCNARANLDYETLRSLRNNGLRLLVVGFESGNQQILNRIRKGVTLDTSRRFMQHCR
                     ELGIKVHGTFIIGLPIETRETIEKTIRFAQELDPYTIQVSIAAPFEGTDLHAEAVKNG
                     WIDNGTLVAENGIQMSTLHYPDLDVDEIEEAVERMYRRFYFRWKPILRMFREMATERH
                     MLVRRLREGREFLDYLHTRKRIGGHLSAAPGTCADDPRR"
     gene            1848..2558
                     /locus_tag="PLM79_02040"
     CDS             1848..2558
                     /locus_tag="PLM79_02040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV85111.1"
                     /translation="MDRPPLYDEFKGCKGILLDFGGTLDSDGEHWLDRFYDLYEKEGV
                     DVSREEIKRVFYRADALCCGDPFVSGLGLRALMRHHVRLQLEELGRSVPAWEEPLVEG
                     FCSRTESFLRRNALLLARLRERFLLGVVSNFYGNVAVLCQEAGLSPWLDVILDSTRVG
                     VCKPDPGIFRMALDGLGLEPAEAVFVGDSYERDMMPARKMGMRTIWMKGPRPRILRDA
                     EPVDGCISNLTDLELLLT"
     gene            2555..3262
                     /locus_tag="PLM79_02045"
     CDS             2555..3262
                     /locus_tag="PLM79_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOV85112.1"
                     /translation="MRMGIIAAGAGERLARGGVAIPKPLVPIGGKPLIARIIEAAARI
                     GLSSVACIVNDLTPATAAYLRATPWPVPVRVLVRTTPSSMESLFALRPFLEDGPFLLS
                     TVDVVFGPDTLASFHREVIPLTEAAGVLALTRFIDDEKPLYAAVDEGGRVTALGERAS
                     GSEFITAGFYAFRPEIFSLIEEARAGRMTALRQFLGLLVQRGLPVYGIPVSKTVDVDY
                     PEDIAKAEAFLEEIGGR"
     gene            3259..4071
                     /locus_tag="PLM79_02050"
     CDS             3259..4071
                     /locus_tag="PLM79_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85113.1"
                     /translation="MKRESVLGIYRERVFSPGKVRDDAAILDETLEELSSMGFRVDAV
                     RGEALDGSVPRPSRVLTMAQSPPALALLESWVSLGTRVINSVDSIRTCYRKPLSRALD
                     GAGLPVPPSRIVPLDEALEEISLLPSTPVWLKRGDVHAVREGDVVSVSSREELSRALE
                     HFRRHKVGELLVQGHVEGPVVKFYGVGRGDYFVAFLASTGEDVTPRVAETLGPVAQRA
                     AGAVGLDVYGGDAVLTGNGSIALIDLNDWPSFSRCRRSAARNIALYLQGKAP"
     gene            4156..5496
                     /locus_tag="PLM79_02055"
     CDS             4156..5496
                     /locus_tag="PLM79_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOV85114.1"
                     /translation="MHFEGSYLRPNETLSSRLYEEEQEFVSPGLQEVSQLSRLVIKEG
                     RGCRLRDMEGKTYLDFMAGVAVCSLGHVHPRYVAALKDQLDKVTVGSFTTENRVELLR
                     LIASLTPPGLRRTQLYSGGAEAVEAAIRLAKSYTKKFEVLSFWGGFHGKTGGVLGLIG
                     DAFKKNWGTLYPGLHLAPYADCYRCPFRMTYPECGLFCLEFVRKILENNTAGSLAAII
                     VEPMQGTAGNVIPPPEFVPGLLQIARENDALLIADEMITGFGRTGRMFGSDHIDLIPD
                     IMTIGKGLGSGFPVSGLISTDEITASKPFSKPSSSSSSYGGNPLASTAALATVRTILD
                     ESLVENSRRVGEHLLNGLRGLQEKYEFIGDVRGVGLLIGVELVKDRKTREPLDRSVTR
                     QMFLETVKRGMISMNYKPNFRINPPLVLTREEADEGLAILDEVFRFVADHVPYK"
     gene            5639..6658
                     /locus_tag="PLM79_02060"
     CDS             5639..6658
                     /locus_tag="PLM79_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV85115.1"
                     /translation="MLRGAIIGFGNVAENAHLPVWRRSSQFRIEAVVEPDPQRARKAR
                     ELLPGVRVHPSAEELFRQDGLDFVDICTPPRFHDDLVVASCRAGLHVFCEKPLTTSTG
                     NFERIREAASRARKVVFTVNNWKHAPLWAKTLELIRANEIGEVRTVSMNVLRVSSSGG
                     GASNWRSDPELARGGIMIDHGWHNLYLIFGMVRESPVSVSARMEAPSGRDSGLEETVD
                     VVIRFPRAEARLHLTWCGPCRRNFGTVVGEHGTLSINDDHILVASNGSAPVRYGFPEA
                     LSAGSHHPEWMDRVVEDFAREISDVRRRGTNLMEARRCIQLIESAYRSSREGSCPIGL
                     RDPAG"
     gene            6773..7780
                     /locus_tag="PLM79_02065"
     CDS             6773..7780
                     /locus_tag="PLM79_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOV85116.1"
                     /translation="MIKFHTVCVLLGLGLLVFLIRQIGFQELWSQMSLLGWGLVPLVL
                     IEVVADALHTIGWKHCLPRTLRSLSFSRLFRIRLAGYAINYLTPTATLGGEITKGMLL
                     SLNHTATGAFQGVVVGKLAYTIAQLLFVSLGSILTLWGVDLPRGVWPALLSGSALLAA
                     GVLGFLVAQKFGKLGAVLRWLSGRKRLGKHLAAAAAKATEADEELRLFYRSHPLELPI
                     AVLWHMAGMACGVLQAWYFLYLLSGHPSMHLAAGIWCLGGWFDLMTFAVPLGIGIQEA
                     TRVFALKAVGFDAAMGLAYGVTLRLEQILRSGLGLLCYATLLSEKNGLEVDAAPAKGP
                     N"
     gene            complement(7836..9062)
                     /locus_tag="PLM79_02070"
     CDS             complement(7836..9062)
                     /locus_tag="PLM79_02070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOV85117.1"
                     /translation="MIATAIILAPDGAGVQKLFGVPALRRLVVILKQLGIGEIHLVGR
                     VAPLVKVVSDLLPSAFLHQLEDGESPEAAIERIELSRDERVLVLRADHVVDRHTLRFF
                     RQSTPGDAPACLTAAGTERLEEGIFLVDRSRLLPVVRLLWQGEELDDNAARGMRHIRA
                     RGPIPRTLDGAGNTPDASEDALAAALGLQTHADDGFIARHFDRHISRRISSRLARTPM
                     TPNGVTLVGMTIGLTAAFLLSLPGYLPHLLGSLLFVFCVIVDGVDGELARLKLQESRF
                     GHYLDITTDNLVHLAIFVGIAFGLYHDTGNARYLRILWVMLGGFGLCLLAVYQCILRL
                     SEDQLKASPNVIRFMALLSNRDFAYLIALLALVDRLNWFLIGSALGSYVFAAALWIIR
                     YREGRRAAPGAAPNIR"
     gene            complement(9263..10450)
                     /locus_tag="PLM79_02075"
     CDS             complement(9263..10450)
                     /locus_tag="PLM79_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOV85118.1"
                     /translation="MPDPLQTPLHSWLVALRRELHQWPEPAYQEVRTAAVVSRVLHEL
                     GIPHRTGVGGTGVVAELKARNKGPTLALRADMDALPLEERNEVPYRSRNPGIMHACGH
                     DGHMTTVLGVARWLLEQEEWVDQGSGKVLLLFQPAEEGGAGALSMIKDGALEGETVEA
                     IFAAHMHPDLPAGEVGLAMGVSNAASDSFHIRLEGKGGHGAHPHQCADPIVAGAYLVT
                     QLQSIVSRNIRPTDAAVITIGIFHAGSASNIIPSEAILHGTLRTLDPQVRETVLTRME
                     EVLEGTRSAHGVSGTLRVTPGYPILINDERLTRFTLETARGLLGPDRVHVEPPRMGAE
                     DFAYFLERIPGVLVRLGCSVPGATVCHGLHSPLFDMDERALDVGVRLFARLLVDYVRS
                     TSP"
     gene            complement(10613..12166)
                     /locus_tag="PLM79_02080"
     CDS             complement(10613..12166)
                     /locus_tag="PLM79_02080"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOV85119.1"
                     /translation="MKTVAERIQELREREAKIKEMGGPKAVAQQHERGKMTARERLDY
                     FFDPGSFREIDMFVKHRGTLFGIDKVEIPADGVVTGFGNVNGRKVFAFAQDFTARAGS
                     LGEMHAKKICKVMDMALKAGKPFVGFNDSGGARIQEGVDSLSGYGQIFYRNAISSGVI
                     PQVSAIMGPCAGGAVYSPAMTDYIFMVKGSSYMFITGPDVIKAVLGEIITQEELGGAM
                     AHASKSGVCHFACESDADAIDQMKKLLSYYPDNNMEDPPCVDMGDDPNRMCPELDSII
                     PDNPMGAYDMKDVIRSIVDAGEFFEIHEHYARNIIVGHARLNGRSIGIIANQPRVMAG
                     CLDCDSSDKATRFIRCCDAFNIPLLTIADVPGYLPGTQQEWGGIIRHGAKLLWCYSEA
                     TVPKVTLMTRKDYGGSYLAMCSRDLGADISLAWPSAEIAVMGSEGAANVIFRREIKEA
                     ADPVAKRKEVIANYQELLYNPYIAASRGYIDGVIVPSETRKRLIEIFEMLSNKREALP
                     PKKHGNIPQ"
     assembly_gap    12292..12391
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12844..13299)
                     /locus_tag="PLM79_02085"
     CDS             complement(12844..13299)
                     /locus_tag="PLM79_02085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HOV85120.1"
                     /translation="MHIYFVETRSEEQKRLLCRWVEHFYEEGRKVQVVTDGTLGAQQL
                     DQLLWTFSQASFVPHRIVSSPNQPPGREAVAVTAGEVPLEGYHVVVCDGPVSREFLGR
                     FPLALHFVLLDDAQRREHSRALWQWAREQGHTVRHIPQGAGVSPLSGPP"
     gene            complement(13315..13656)
                     /locus_tag="PLM79_02090"
     CDS             complement(13315..13656)
                     /locus_tag="PLM79_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HOV85121.1"
                     /translation="MISGYQFGRILIEGKAYRSDLKILGGKVLPDWWRKEGHEVAPED
                     VEDILSAQPEILVVGMGEPGRMQVLESLRSRLVQANIQLLEEPTSKAVESFNRLTREG
                     RNVAGAFHLTC"
     gene            complement(13803..17294)
                     /gene="dnaE"
                     /locus_tag="PLM79_02095"
     CDS             complement(13803..17294)
                     /gene="dnaE"
                     /locus_tag="PLM79_02095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOV85122.1"
                     /translation="MNPFVHLHVHTQYSLLDGAIRLKDLLETAVGYQMPAVAITDHGN
                     MFGALEFYDMARKAGIKPIIGCEVYLAPRSRLDRHTGGELTANGEEDRNYHLVLLAKD
                     LTGYRNLLKLVTRAYLEGFYYKPRIDKALLRQYHEGLIGLSSCLKGEIASLLLRNQDG
                     TARKTALEYAEIFGPENFYLELQANGIPEQDAVNETLIRMGKDLGIPLAATNDCHYLR
                     KADARAHEVLLCIQTGKTILDEKRMKFQTDHLYFKSPEEMARAFSHVPEALENTLVIA
                     ERCNLDLQLGEYHFPDFPLEPGETLEDRFEKTVMEGFEERIGQIRSTRPDFTDKDQQE
                     YRRRLRLEMDVIRRMGFVAYFLIVADFINYAKQKGIPVGPGRGSAAGSLVAFVMKITD
                     LDPIEHGLIFERFLNLERISMPDIDVDFCVHGREEVLRYVTEKYGQDRVAQITTFGTL
                     QARAAIRDVGRALAMPYNEVDRIAKMIPASPGMTLQKAFQAEPRLGEQQRNDPQIKEL
                     FEVALALEGLTRHASTHAAGVVIADKPIVEYMPLYRGQDGETVTQFPMKYVERAGLIK
                     FDFLGLRNLTVIHNAVQLIRKNHHLDIRPEALPLDDPETYALLSRADTTGVFQLESSG
                     MRDILVRLRPENFADIVALVALYRPGPLESGMVDQYIDGKHGLAPVTYDLEMLRPILE
                     PTHGVILYQEQVMKIASVLANYTLGEADILRRAMGKKIPDVMKAQRDRFISGAKENSI
                     DLAKANHIFDLMEKFAGYGFNKSHSAAYALIAYQTAYLKAHFPVEFMAALLNSFLSNS
                     DQVVKLINQCRENQIEILPPDVNASSKDFTVVEGKIRFGLGAVKNVGETAIESILTVR
                     DQNGHFRSIYDFCRRVDTQKVNRRVLEQLIKCGAFDTIHGNRAQVLAAADDALEKAQV
                     VQRDRQTGQMNMFELLRSQKGKTPALPTVEPWDNRTTLQFEKEALGFYISGHPLDFYA
                     ERIARLCTADTQSVSEKREGVEVALCGMVTITKELTTKRGDRMAFLSLEDKEGILEVV
                     AFSEAFLQARPLLDKDEPLVVLGKVQHDEKGTKLVANRILTLAEAQLESVDAVRIRLR
                     AEDLDKDALSRLRHLLMSHAGDCKTFLHLVVDSKAEAVIALNPKLSVNPARGFFEEIA
                     QQFGSDCAEVVYKTTVH"
     gene            17606..18094
                     /locus_tag="PLM79_02100"
     CDS             17606..18094
                     /locus_tag="PLM79_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOV85123.1"
                     /translation="MPKLPGRMLGLAVGFLLLWSVPGDSSPLPSAPKKNSGQNSDLVH
                     QVKAGALFDYAKQENKRLRWDECLARKALVRARKMVQDGYFSHQDPKTGKNPAWKYVT
                     ACHKVRYAGENLAMGYESAKTLHEALMDSPTHRKNIVSRRHTLLGVGCYDYICVQLFA
                     GF"
     gene            18250..18325
                     /locus_tag="PLM79_02105"
     tRNA            18250..18325
                     /locus_tag="PLM79_02105"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18283..18285,aa:His,seq:gtg)
     gene            18718..19134
                     /locus_tag="PLM79_02110"
     CDS             18718..19134
                     /locus_tag="PLM79_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85124.1"
                     /translation="MKWEAKGISAILLALILGMSMGALFQGDRADAAESKLLQGEEWA
                     KMSLESKRAFILGVGEMADIEEEMIGKYPELKRDSFSEKVAEALGDVPLDTVVNRIDA
                     FYKANPAKMNVPVMRVVWDEMIKPKVSTIAGKPVKK"
     gene            19224..19454
                     /locus_tag="PLM79_02115"
     CDS             19224..19454
                     /locus_tag="PLM79_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HOV85125.1"
                     /translation="MKRNRIMALMLVVACLFTFGCAGMSETQQRTLSGAAIGAAGGAA
                     LTAIAGGNPAVGAGVGGAVGGLTGYIIGEQHK"
     gene            complement(19869..20366)
                     /locus_tag="PLM79_02120"
     CDS             complement(19869..20366)
                     /locus_tag="PLM79_02120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOV85126.1"
                     /translation="MSEEKYRLLPHRESDNCFACSRTNPSGLKMNFYTDGQKIVSRVR
                     VAPNLSGWDQLVHGGILATMLDEAMGWGVLHLVGRITLTKSMTVDFLKPAYVNRELKV
                     ESHILERRGDREVWMEGFLWNDAGELCAKSLGSYALFTAEDMKRRKILNEELIRDVER
                     IIAGG"
     gene            complement(20456..20926)
                     /locus_tag="PLM79_02125"
     CDS             complement(20456..20926)
                     /locus_tag="PLM79_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85127.1"
                     /translation="MLACTDCSNFFESNLVKPDKDGDLVCPVKDCDGYLVDLDDLLAD
                     LLTRFWSLGHWTRYSCAGHLWRAEFEPHIAFSLDEENLKLKEIIEGMNDGTVRIETSE
                     EEGEESGEEPKLVVLAAAAASSDPIERLQIQHDFLEFMYEVLLRLEEEADNEEA"
     gene            complement(21018..21611)
                     /locus_tag="PLM79_02130"
     CDS             complement(21018..21611)
                     /locus_tag="PLM79_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85128.1"
                     /translation="MNLIMMYQLMAALLVLVTLLSLGYYLGYRKNVRKYQALSANDRE
                     EMLTIERALKRFWDHEKEQLEAEKAELERRIQFLEDRLDQYRRKAAGIGMMGLRKSKL
                     TDMLLSLLVENETLEERLFMQNLKLKQERDQYLENELRHISYKRILLSDLINQAGVRK
                     EMERVLNEKGKLKRLEIRGREMEPSPLPQKNEEDTAY"
     gene            22196..23152
                     /locus_tag="PLM79_02135"
     CDS             22196..23152
                     /locus_tag="PLM79_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85129.1"
                     /translation="MMLVQRIKLEDLVCMEDPEDVFEECKRLLFLMFPNYSQQPLEKA
                     FEHVRGLFLGRCPGYFRCNTRYHDLKHTTDCLMALIRLIHGAHRTGVQMEEGMVTLAL
                     ITALMHDTGYVQTADDVSGTGAKYTLNHIERSICFMKEYFDAQGYSAQEFAACRDILR
                     CTGLDVQMTEIRFQSGEHEMLGMMLGASDLLGQMADRTYLERLPFLFQEFKEGGVPGF
                     ESELDLLRKTPGFWEFTKKRFETQLGNVDRYMLHHFLTRWGIDSDLYREAIENNISYL
                     KYILEQHPDNYSDYLRRGGLIKLLRERQFRADTRGEACKGEE"
     gene            23532..25610
                     /gene="shc"
                     /locus_tag="PLM79_02140"
     CDS             23532..25610
                     /gene="shc"
                     /locus_tag="PLM79_02140"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HOV85130.1"
                     /translation="MRGIRETLAEVPEGDDERENFRIVLKGSEHPLDEKTFSHLRPRV
                     EKGREPVPHHSTRHSSLQSSWIRSIDKAIQSTTQFFLRTQYPEGFWWSELESNVTITA
                     EYVMLLYLLGRLDPSRGRRMAEYLLNKQSPNGSWPLYYGDEGNLSITVEAYFALKLLG
                     EKSDSEPLRRARSFILNNGGLEASRVFTKIWLALFSQYDWDKVPSMPVELVLLPSHFY
                     FSIYEFSSWARSTAVPLSIVLCIRPTHRLPREKSVSELHPTPGSATRKKRFASMTHKL
                     FFLFDRVAKWLEKHPLPDLRRRAIRTAETWILEHQEETGDWGGIQPPMVYSILALHYL
                     GYPLNHPVLVKGMRALDDFCIEDDLGYRMQSCISPVWDTALTALALMESGMPPTHPTL
                     QRVQQWLIQRQVKTGGDWQVKNCCPPGGWAFEFVNTQYPDVDDSAVVLSALHRLGSTP
                     CAGLEESKSRGMEWVLSMQSSCGGWAAFDRDNTMTILNRIPFADQEAMVDYPTADVTG
                     RVLEAMGYYGYQRSHPRAQKAIRFLKDLQEPDGCWWGRWGVNYIYGTWSVLRGLIGIG
                     EDPKLPYIQAALQWVKDHQNPDGGWGETCESYCRPELRGQGPSTPSQTAWAVMALLAG
                     GEHGSPEVRKGVQYLLRNQQPDGTWEEIHFTGTGFPNHFYIRYHNYRNCFPLMALGQY
                     LQTLRDRPSS"
     gene            complement(25626..26483)
                     /gene="panB"
                     /locus_tag="PLM79_02145"
     CDS             complement(25626..26483)
                     /gene="panB"
                     /locus_tag="PLM79_02145"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOV85131.1"
                     /translation="MSKRITAPDVLAAKGKRKLTMLTAYDYPSAVWVDESGMDMILVG
                     DSLAMVVLGQEDTLSVGMEEMLHHTRAVARGAKRALIIGDMPFLSYQCGVEQAVLNAG
                     RFLKEGRAQAVKLEGGKRVVPQVEAIVAAGIPVQGHIGLTPQSSAQLGGFKVQGKTAG
                     AAKALIEDAQALAEAGCFSIVLEAIPSPVAARITETVPVPTIGIGAGPDCDGQVLVIH
                     DVLGLYDRFLPRFVKQYARLGKPIREALTLYREEVEQGRFPGPEHTFAMAEEEKTKVA
                     KMKGKKKGP"
     gene            complement(26501..27445)
                     /locus_tag="PLM79_02150"
     CDS             complement(26501..27445)
                     /locus_tag="PLM79_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOV85132.1"
                     /translation="MHVLILGAGALGSLLGARLSRTDARVTLLSTNRQHIEAIRRKGL
                     LVDELDGTQQRFELVSHYDPAAITEPADLVIVLVKLYDTLQAVPTVASCCHSKTFFLT
                     LQNGIGNWERIASLVGIEGVLVGSTAQGSTLVEPGRVRHGGNGLTYVGEPQGPPSERV
                     YGVVDLFRKARLQTEASDRMEQLIWEKLHVNVGINAITALANIRNGLVADVPTARDLC
                     TRAVREAMEVAAAKGFVIQKDMVERVLNVARATAVNRSSMGQDVDRKKRTEIDGINGA
                     IVELGRRLGLTLPVNETLTQLVKTLEAGYLQPPPPQTE"
     gene            complement(27585..28022)
                     /locus_tag="PLM79_02155"
     CDS             complement(27585..28022)
                     /locus_tag="PLM79_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HOV85133.1"
                     /translation="MEETKTAPVTKEPLKPQDLFDFSDERVFFAWQRNHMANERTFLA
                     WCRTGISLIVFGFVIERFDILIREIQAFSSNVKRVSQTHYLGLVTFGLGAMIIILAGW
                     RFFYIRSHINRGETGFSPLPDVFLMVSVLVTVLVIFLLFIFLF"
     gene            complement(28157..29266)
                     /gene="mraY"
                     /locus_tag="PLM79_02160"
     CDS             complement(28157..29266)
                     /gene="mraY"
                     /locus_tag="PLM79_02160"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOV85134.1"
                     /translation="MIYEICNILIPLSDVFSFCRLVNYITFRAIMAALTSVIFIFLVS
                     RHFILFMHHRSLFDQVRDTGVHSAFDKSGTPTMGGVLIIGSVLSSMLLWGNWHNPFLL
                     CSVVGMLWFGGLGFMDDLSKVKRKSGDRGLSERTKLFLQGLFALLFAWVCVGPYAPLG
                     APLATKLYIPFYKHAVWDPGPYFYGAFIFFFVIFVSNAVNITDGLDGLAITPSLFVVG
                     VLGIFAYVEGNRIYSAYLYYPYLRGVSELTIFGAAFVGGGLGFLWYNAYPAQIFMGDT
                     GSLAIGGIMAVISVLLKQEMLFPLLGGLFLAEALTSQFQDKVGVRWLGRRVFFRAPLH
                     HNLQYKGNAETKVVIRLWIVAGILALISLATLKLR"
     gene            complement(29335..31443)
                     /locus_tag="PLM79_02165"
     CDS             complement(29335..31443)
                     /locus_tag="PLM79_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85135.1"
                     /translation="MDPSLMILLVLLTASAVVFLCARRWKTLARREVRVLLTGGGTGG
                     HVNPALAIAEDIKKRDPCAKFLYVGVRGKAESVIVRRAGYPLWFVFSEGFPGLKPSFR
                     CVRFLFKLGLGVVQSVAYLLIFAPRWVIATGGYVSAPVILAALILRTLRVSPAKVFLH
                     EQNSIPGQLNGLLGRYVDRVLLTFPQTLSFFPRNGVVTGYPIRKSIAVKPRQEALESL
                     PFRIPDGKRVVFAFGGSQGARTINRAVVDSLPHLLPHGREIFIIHGTGLARSADYDAA
                     ADTLDRIEKTLTPEQKNSLAQCYYRQDYFHNIADIYSVCDLIVCRSGAGSLNEISCVG
                     KPAVLIPKANLPGDHQVMNARTMKHCGAAVILFEDTVVEAGRILEKLEGRVLAECILQ
                     LLKDPSTLEQMARNSRSFLRQQANDRILNEIYGKNQAVASSELDLVKVPFRPLLSNQQ
                     LLQTLSAAYRRAPDQYHPLSVIGDENDLHYYQHRASGLLSHEAWQDRNVGVKLTGLTL
                     HREKIPTLLHMLTDRTPVHWVKRILGGDFEQVGFIRRNIIQAIQVMGHFDAEVEQALL
                     TAMEDPYYEVRSQACAALARFGSYGSGKEEWFQALVKRLNEPCFEVVVQAAEALGEIG
                     TNSRALEILLRLREHHYWQVRDAALKGVKRLLERGTITPSPHLSSELLGFILTATDFR
                     PHFSIKQTYKSVRDLLNSEP"
     gene            complement(31513..32169)
                     /locus_tag="PLM79_02170"
     CDS             complement(31513..32169)
                     /locus_tag="PLM79_02170"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOV85136.1"
                     /translation="MKYRDRIWRINGSGPPDPFIAEAAARLARDGVVIYPTETFYGLG
                     ANPFSADAVLRAYRIKRRSLAKAMPLIASDIAAVRNVAADWSPSTDLLVRAFWPGPLT
                     LILPTAVPFPSMLLTGPGKIAVRVSSHPVAGALATAVGGLVTATSANIAGSSPIADPA
                     EIPPDLLREVDGMLDAGTLPGNLPSTVLDVSRPVPRLVREGCLPAELIARALSRRTSW
                     "
     gene            complement(32177..32671)
                     /gene="purE"
                     /locus_tag="PLM79_02175"
     CDS             complement(32177..32671)
                     /gene="purE"
                     /locus_tag="PLM79_02175"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOV85137.1"
                     /translation="MGSDSDLPVMSGAIQVLEDFGLPYEVQILSAHRCPDETASYARE
                     SAAKGLKVLIAGAGWAAHLAGALAAQSTLPVIGIPIDSSPLQGLDALLSTAQMPPGVP
                     VATMSIGKGGAQNAALFAVQILALLDPNLARKLENHKQDMKRDVLLHKNNKLQEHLSE
                     RKTS"
     gene            complement(32750..34045)
                     /gene="purD"
                     /locus_tag="PLM79_02180"
     CDS             complement(32750..34045)
                     /gene="purD"
                     /locus_tag="PLM79_02180"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOV85138.1"
                     /translation="MKILVIGSGGRDHAIAWKFAQSPRVKHVYVAHGNAGIARTATCV
                     DARSIEDMAAFAEKERIDLTFVGPEKPLSAGIVDLFESKGLAIVGPDKRTSCLESSKC
                     DTKILLRDLGIPVPDFEVFSDPQKAKDYVGQVGYPVVVKADGLAAGKGSLVCDSVEDA
                     REAVRAVMEERIFGDAGARVDVERRLYGRELSFFCFTDGYSVVPMVAAQDYKRAREGD
                     RGKNTGGMGAYSPHPWLDDALVRTIMARVAEPLIHGIREKHGIVYRGILYLGLMLVNE
                     SSGIVPYVLEINIRLGDPEAQVILPRLETDLVDICEAILTGRLAQTRLVWDPGYRLCL
                     IAVSGPTKGKKGWYKGYPDRYKIGLPITGLDQIDPRVLVFHSGTETDSNGTWITTGGR
                     VLCLVSRGETLAEARAVALEEMKKISFEGLYFRSDIGLE"
     gene            complement(34168..34779)
                     /locus_tag="PLM79_02185"
     CDS             complement(34168..34779)
                     /locus_tag="PLM79_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOV85139.1"
                     /translation="MFPIRRALISVTDKTGLVDFARGLERWGVHLLSTGGTARLLRDN
                     GIAITEVSDYTGFPEMLDGRVKTLHPKIHGGILGIRDNGEHLETMRKHGIEPIDMVVV
                     NLYAFAKTVARPGCTLEEAVENIDIGGPTLIRASAKNHRHVAVVTDPEDYPLILEEMN
                     GHGGSLSRKTSMSLACKAFCLTHAYDGNICEYLRSVVNGSARD"
     gene            complement(35190..35948)
                     /locus_tag="PLM79_02190"
     CDS             complement(35190..35948)
                     /locus_tag="PLM79_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV85140.1"
                     /translation="MGFTVALGGKGGTGKTTIAGLLIRYFIKHGMKPVLAIDADSNAN
                     LNDVLGVCLDETLSDAREMMKTSVPTGMTKDVFMQMKMEQSLVEAEGFDLVAMGRPEG
                     PGCYCAANNLLSSLMDRLIKNYRYLVVDNEAGMEHFSRLTQKDIDFLLLVSDPSRRGL
                     AAACRIAELVRTLPMKVAEVRLLVNQMDSSPPSWPEEVIQTFGEDHIHVLPKDPLVAQ
                     YDFEGKPTLTLPEDALIVKAADSIFDALFSRNRE"
     gene            complement(36019..36444)
                     /locus_tag="PLM79_02195"
     CDS             complement(36019..36444)
                     /locus_tag="PLM79_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85141.1"
                     /translation="MSGSMRRISIVFAGGVFGAFVNSLLAWYLGRKGIPQQFGVAIAP
                     AWSAQYLYPRLVWGGLWGLVFLAPAWRSGFWVGVFARGVLFSLLPTLFQLLYVFPVLQ
                     GKAVLGLTLGKLTPVFVFFYNAVWGFCAALWTYGSKGDA"
     gene            36731..37354
                     /locus_tag="PLM79_02200"
     CDS             36731..37354
                     /locus_tag="PLM79_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV85142.1"
                     /translation="MDLTVRQAANRLKVSESKVRDYIASGTLPTRKKGRGTVIPEDAV
                     EGLLKQSPPGDAMPPGPDETLRDMLASLLQKVAELERQVDEKWSLWRENRRLLEELNQ
                     SRRQLAEKTSEVERLQRDLLYQKRLLEKELQDCRTAMEEKGAWIEKQCRERIAGEREL
                     LLDRLALQEKHLEEKLEWEKEKYGRKLLEIQQREGFWSKLARMMTWS"
     gene            37450..38022
                     /gene="pyrE"
                     /locus_tag="PLM79_02205"
     CDS             37450..38022
                     /gene="pyrE"
                     /locus_tag="PLM79_02205"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOV85143.1"
                     /translation="MKNRLIELILDYAFQYSEKPVFRLVHGGMSRFYFNCKQVTLDPE
                     GQYLIGHLVLEKIRDLQVRAIGGLTLGADPIANAVSYTSWLRKDPIQAFVVRKKTKDH
                     GTGSLIEGKVRPGDRVVVVDDVITTGGSTIQAVEACRSYGLEVVKVVVLVDRQEMGGR
                     ESILQTVPALDALVTREEIMQVYGEGVIHD"
     gene            38015..38485
                     /locus_tag="PLM79_02210"
     CDS             38015..38485
                     /locus_tag="PLM79_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85144.1"
                     /translation="MTDEPKGTQDACIEPWEGERAEAANRICCRACDATGVKVSSWEQ
                     FDAWKQYVDGKIEEAELTEQARRELDEFSTVFGKYLVIEQEQPKQTDKEAEKKKRARR
                     ANAIYREVCREAGLSVYFFHDFLSWSDFVAGNIGESEFYDRARREVMEIRGRPH"
     gene            38521..39003
                     /gene="moaC"
                     /locus_tag="PLM79_02215"
     CDS             38521..39003
                     /gene="moaC"
                     /locus_tag="PLM79_02215"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOV85145.1"
                     /translation="MRERELTHIDSSGRLRMVDVTEKNITEREARAEATLRLSRETLR
                     QILDRKVPKGNVLEAARLAGILAAKKTWELIPLCHPIHLSGIRVDFLVEEERSEIKVL
                     STVKALDRTGVEMEALVAATHASLTIYDMCKAIDREMSIHEVRLTYKSGGRSGTYQRA
                     "
     gene            39081..39443
                     /gene="dksA"
                     /locus_tag="PLM79_02220"
     CDS             39081..39443
                     /gene="dksA"
                     /locus_tag="PLM79_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOV85146.1"
                     /translation="MDREMQAVFKELLLKRLDELYQEAEKTVAGMTDTEETFPDPTDR
                     ATLESDRNFMLRIRDRERKLIMKIREALQRIEDGNFGICEMCGDDIGIDRLKARPVTT
                     QCIECKRKQEAAERARGG"
     gene            complement(39447..40481)
                     /locus_tag="PLM79_02225"
     CDS             complement(39447..40481)
                     /locus_tag="PLM79_02225"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOV85147.1"
                     /translation="MMVAQDPTHWQILQLLKDQKGGYLSGSFVATGLGLTRTAIWKHI
                     QNLRQMGYDIESHPKEGYRLLDVPDLLIPEEILPNLETQWLGRSYHHFSRVASTNDHA
                     LLLAARDAPHGTVVVAEEQTHGRGRLKREWLSASQKGIYFSMLLRTPLPIRIASQTTL
                     VAALALAKAIRTRYRLPSAIKWPNDILIRNRKVAGILTEIQSDLDFTRFQVIGVGINA
                     NYTAAELGGPFRYPASSIAVELGRSIRRQELLLEYLHEFEREYSRFIREGFETLLTEL
                     ERYSAVLGKTIRILRGEEELEGKVLGFTPEGALRLLTEDHVEKIIWVGDVTQVLGSVP
                     GESADGALES"
     gene            complement(40630..41382)
                     /gene="cobS"
                     /locus_tag="PLM79_02230"
     CDS             complement(40630..41382)
                     /gene="cobS"
                     /locus_tag="PLM79_02230"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOV85148.1"
                     /translation="MWCDFVTALSFLTLLHVPSMTGREPTPVSLARSFSFFPLVGFLL
                     GGGSWGLALLLARTTPPFLLACILTLYSVLVTRAFHMDGLADFWDGLGGGYNPDRRLE
                     IMKDSRTGAFGAVALSLAVALKIAALHALLTHRLGSPLLIVPAFSRLAMVLTAYKTPY
                     ARKEGGLGKPFLEHMSARELRIALGICLLLSAVVNPFFTLLCAATVLVFSRVMKRGCR
                     KWFGGVTGDVLGAVNEMTEILLYALGAMVLAS"
     gene            complement(41512..42093)
                     /gene="cobU"
                     /locus_tag="PLM79_02235"
     CDS             complement(41512..42093)
                     /gene="cobU"
                     /locus_tag="PLM79_02235"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOV85149.1"
                     /translation="MSAPDLPSTPHLILGGAKSGKSKWAEELITAFPPPYIYVATAQA
                     LDEEMKERIRIHRERRSSFWETLECPIRLTETLGNLQGRNLPVLVDCLTLWLSNLLLR
                     FSPGVVESQITELRGFIGQVDYPLILVSNEVGAGIVPDNPMARRFRDLAGTANREFAG
                     ACASVTYVVAGLAWVLKGPNLTPPGIAGHSEKA"
     gene            complement(42090..42524)
                     /locus_tag="PLM79_02240"
     CDS             complement(42090..42524)
                     /locus_tag="PLM79_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HOV85150.1"
                     /translation="MIRIAVIDGQGGGIGTTIIRKIKDVYGERTEVWALGTNAIATAQ
                     MMKARANRGATGENAICHCVNEVEVIIGPISILLANSMMGEVTPGMVRAVGAAKATKI
                     LLPITQDPIVIVGTVREPLPHLVDKLVLEHLRGFFPQDIESS"
     gene            complement(42603..42872)
                     /locus_tag="PLM79_02245"
     CDS             complement(42603..42872)
                     /locus_tag="PLM79_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85151.1"
                     /translation="MEYERVQVLTRDEYREAQEPISFQWRNNRYRIREIVDRWYSGYL
                     DARRVPLRYFKVKTQDGKEFILRYHELFRAWSLLVPPGEEEERDP"
     gene            43134..43670
                     /locus_tag="PLM79_02250"
     CDS             43134..43670
                     /locus_tag="PLM79_02250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOV85152.1"
                     /translation="MARKALLVIDVLNDFLDPKGALYCGDDSRKIIPPIRVLLDRFAR
                     DKHLIVFLRDAHSVDDKEFELFSPHAVKGSWGSQVIPELAPPEGSLVVDKTRFSGFFG
                     NDLDKILSSAGPEEVWVAGVVTSICVMDTAGDLRNRNYSVVVPMDAVADFDPKFHEFA
                     LTRMQRVYGARLVKTTED"
     gene            43711..45075
                     /locus_tag="PLM79_02255"
     CDS             43711..45075
                     /locus_tag="PLM79_02255"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOV85153.1"
                     /translation="MSASRRYAPELVTDLYELTMAASYLREGMIREATFSLFIRDYPP
                     DRAYFVCAGIEDLVETVTDLNFSHSSLEYLSSLEKFPREFLDYLRRFRFSGTIRAMPE
                     GRIFFAQEPILEVTAPLIEGQILETLILNTIQLETLIAGKAARCVHAARGRGLIDFSL
                     RRTQGIQAGLKAARGSYIAGFLGTSNLMAGEWYKIPVFGTMAHSYVTSFPSEMDAFVA
                     YARAFPDNTVLLIDTYDTLSGARKAVQVAKRMRDEGKQLVGVRLDSGDLAELSKQVRA
                     ILVGEGFPDVMIFASGNLDEYKVEELVRAGAEIDIFAVGTRMGVSADSPYLDIAYKLV
                     EYDGRPVLKLSAGKKTWVGRKQVYRSRDGEGKMKEDRLALAGEKVPGAEPLLETVVEG
                     GRLVHPLETPATIRERFRQEWEGLPPIYRELRPRHRYPVSIGAELGALQEKTIREKSL
                     QEID"
     gene            complement(45103..45777)
                     /gene="rsmG"
                     /locus_tag="PLM79_02260"
     CDS             complement(45103..45777)
                     /gene="rsmG"
                     /locus_tag="PLM79_02260"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOV85154.1"
                     /translation="MKEDPFVSLLLRKCREHGIAFSEEQAHRVFQHVELMLEWNRRVN
                     LTRITEPEQILNEHILDSLIPAQWLPREGSILDVGSGPGFPGVPLKIFSSHRTVVLLE
                     SNRKKVSFLRVLLARLGMKSIAAVQGRWEEWERLAVPGIPRKYELIALRSVRLDPRRF
                     GDYARDSLGPGGVLAYWGGELAFPPESLHESTPSPGSLRFRGKHPYTVPGLHKPRYLW
                     LWEKGL"
     gene            complement(45779..47179)
                     /gene="mnmE"
                     /locus_tag="PLM79_02265"
     CDS             complement(45779..47179)
                     /gene="mnmE"
                     /locus_tag="PLM79_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOV85155.1"
                     /translation="MGPEAPDDTICAVATPVGEGGIGILKISGAGAFQVLQRLFRPHK
                     SVGTLQSHRLHHGWIHDPESGQAVDEVLVSYMAAPHTYTREDVVEINCHSGYWVLDHI
                     LQLVLKSGVRLAEPGEFTRRAFLHGRIDLSQAEAVIEVIRSKSHHHLLMANRSLRGGC
                     RTRVQSWRESLLELQARLEASIDFSDDLEEGEASGWEAWAESLEQDLCRPLTRYLEEY
                     ESTRVLREGLTLVLVGKPNVGKSSLLNVLLGKDRAIVTPLPGTTRDVIEDTFLLSGIL
                     VRILDTAGIRREPDEIESLGIERTLRSVDEADIALWLIDRSSSLTPEDDAVFEALSGI
                     PFMILLNKSDLSPHLSPEDVRKRYGGSPLIMPLSVLNPRDIERLRETLADRYLSRPLD
                     TRRSEFIPNLRQKECLEGALRALRETQKLLASGKDTELACLELDSARRQLDAILGREP
                     DDELLDRIFSQFCIGK"
     gene            complement(47208..47876)
                     /gene="jag"
                     /locus_tag="PLM79_02270"
     CDS             complement(47208..47876)
                     /gene="jag"
                     /locus_tag="PLM79_02270"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOV85156.1"
                     /translation="MPTLEIEEKSVEDAIALACERLKLPREKLEIEVLSKGSSGIFGI
                     VGAKKAKIRVTSKVTAPEAIAERAKEVLVEVLKYVDLPTVVEPDAHEDHVCLNIISNG
                     SGILIGKRGKTLNALQYLVGKIVQKEMGENISVIVDTENYRSKREVSLTELAQQLSEK
                     VKKSRRPLTTGPMNAQDRRVIHLALKEDEEVRTKSKGEGNLRRVVIYPVKRGKKSPEV
                     AAQE"
     gene            complement(47945..49606)
                     /gene="yidC"
                     /locus_tag="PLM79_02275"
     CDS             complement(47945..49606)
                     /gene="yidC"
                     /locus_tag="PLM79_02275"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOV85157.1"
                     /translation="MEKRAIIAFVLSLLVLVFWDYYFGLFRAPDTKPPQESAPSGPVS
                     QAPRPTPKLPDTVVPKVLPRGQVKLDQHFPSWTVDAPLFSTQLLAPGARIHSVSLKKY
                     RREVNPDSPPMQIVTTQPTGYLPMAVDLMNHSDWSLATRPFSSPAPEQIRLTPENPSQ
                     VLALQTEIPGEVRVTKSYTFSADAYFVDVEIQLENLSEKTLSDQMGISFYFQPYSPLA
                     SESSYNPSQLSSHEKGKIANHSLSDLEKKELVLKGPIDWVGYENNYFIQAVLPVEKQG
                     YQVVARVLDPKEGLLQLVYLTEPFQIEGGQARKIPLRIYLGPKELTQLGKAEHQLSAS
                     VDYGWFTFLAKPLLLGMNWVFQYTHNYGIAIILLTIVIKVIFWPLTQKSFESMQTMKK
                     IQPKITQIREKYKNDKEKLNQELMAVYRTYKVNPLGGCLPMLLQIPVFFALYRMLNAA
                     IDLRHEPFMLWINDLTAPDRLHIGIPIPYLDGVPVLTILMGISMFVQQKMTPATGDPR
                     QDKLMLLMPVVFTVFFVNFPSGLVLYWLVNNILSIVQQYWINHKA"
     gene            complement(49588..49860)
                     /gene="yidD"
                     /locus_tag="PLM79_02280"
     CDS             complement(49588..49860)
                     /gene="yidD"
                     /locus_tag="PLM79_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOV85158.1"
                     /translation="MIRSFFLLAIRSYQTLLSPWMGPCCRFVPTCSQYTYEAVQLHGP
                     WKGCLLGLGRIARCHPFHPGGYDPVDPPSTTDVPTKWSFLRWKNGP"
     gene            complement(49879..50217)
                     /gene="rnpA"
                     /locus_tag="PLM79_02285"
     CDS             complement(49879..50217)
                     /gene="rnpA"
                     /locus_tag="PLM79_02285"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOV85159.1"
                     /translation="MGNYPYRPCERMRSSKDYRQAKRTGKRVRTAHFTINWRPNNLPH
                     HRLGMVVQKRFWGAVQRNRIKRRLREWFRLHKHEIPPPGKDIVFVAGPGVETMLPEGI
                     ARELAEVLTK"
     gene            complement(50276..50410)
                     /gene="rpmH"
                     /locus_tag="PLM79_02290"
     CDS             complement(50276..50410)
                     /gene="rpmH"
                     /locus_tag="PLM79_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOV85160.1"
                     /translation="MKRTFQPSNLKRKRTHGFLARMATQGGRKVIQRRRARGRKRLSP
                     "
     gene            51003..52034
                     /locus_tag="PLM79_02295"
     CDS             51003..52034
                     /locus_tag="PLM79_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV85161.1"
                     /translation="MFLDRIFGWISNDLAIDLGTANTLVYVRGKGIVLCEPSVVAVRK
                     DERGANRVVAVGKDAKLMLGKTPGNIVAIRPMKDGVIADFEVAEAMLRYFIQKVHNRR
                     NLVRPRIIVAIPSGVTQVERRAVRESAESAGAREVYLIEEPMAAAIGAGLPITEPVCN
                     MVVDIGGGTTEVAVISLAGIVYSRSIRVGGDKMDLAVLQHIKRKYNLLIGERTAEQVK
                     TAIGNAYPIGEAETMMIKGRDLVSGIPKTVEINSDEIRESIQEQIDSIVETIKIALEQ
                     TPPELAADIVDRGIVLTGGGALLRNLDHLLRLETGLPITLTEDPLSTVVLGSGKALEE
                     IDILKDVLA"
     gene            52108..52953
                     /gene="mreC"
                     /locus_tag="PLM79_02300"
     CDS             52108..52953
                     /gene="mreC"
                     /locus_tag="PLM79_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOV85162.1"
                     /translation="MMAWLRRLRGTILIVIFLGVFFYIFSLNFRPPERMDVFQRVVIE
                     TTAPLIKALRNVFISTEETIKEYVWLRQTRRENEQLRSRVASLEEEITHYREAYVENL
                     RLRRLLDFKSTTQAEMIAAQVVLHDLTGWFQTVIVDKGFRDNIEPDMAVVSDEGVVGR
                     TLDISDRYSRILLITDPGSSVDALIQRNRVRGILVGKDANGCVLKFVRSNLDVQVGDL
                     IISSGKDGIFPKGLRLGIVQGTFKDPVDLFQKVEVKPLVRLSALEEVLLLKRGAILPE
                     NLLGN"
     gene            52966..53493
                     /gene="mreD"
                     /locus_tag="PLM79_02305"
     CDS             52966..53493
                     /gene="mreD"
                     /locus_tag="PLM79_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOV85163.1"
                     /translation="MNTTNGLGEDRSFVQWQSFLKVTLYSLLLLVFQAVWASRTASAA
                     LKPDLLLPMMLPVAVWWPPVASLGWAILWGFMADTLSGKLWGYHVGSYVVSVCLVHIA
                     AEKFEFQNPLYQMFFVGLCSLGQSLVLGLFLFLEPAGSLLGASAGRDLVGRFVLTMIL
                     SPLIIFPVWKDRASN"
     gene            53516..55441
                     /gene="mrdA"
                     /locus_tag="PLM79_02310"
     CDS             53516..55441
                     /gene="mrdA"
                     /locus_tag="PLM79_02310"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOV85164.1"
                     /translation="MRSLLLRRNRENRAREKESKRAKGNHLLLVNWEMAETDLFQRQY
                     LLLIGLMLLMFVVYGLRLWHLQLVEGAKYRYQSENNRIRLEDISSPRGIIFDRNGTPL
                     VENRPAYHLQIIREDVRDLDQTIRTLAGLCNRSPEEFFGILEAHKSVPKFLPVRLLAD
                     LDRDCLAQIEARRIRLPGVSIQLEPKREYRWNGTAAHLIGYLSEITEAELKSGTYSGY
                     YPGEDIGKFGVEGAFEKYLHGKRGGRQVEVDAIGRRRRLLDEVLPIPGRNLWLTIDIE
                     LQKVAESCLEGKVGAVVALDPNDGAVLAMASSPVFDQEKFIRGLSKSEWQTLSKDPSH
                     PLLNRAIGAAYPPGSTYKAFMAIAALKEGVVTPDTHLHCPGFYQFADRKYRCWRDHGH
                     GSVDVDRALVQSCDVYFYQCGMRLGVDRIAHYANLFGLGEKTGLGLHGEHPGLIPTSW
                     WKKQATGIPWQKGETLSIAIGQGFDLTTPLQLTVGYAAIANRGKLWQPYVVRRIEGNS
                     REEINEVKGKLKRTIPIEQKYFEIVRKGLLGVVEDDRGTAHAIRLKGLPMAGKTGTAQ
                     VVRLGENVNRKAAARAAKEKEKDHAWFVGYGPADHPQIVVCAFVEHGGHGSSTAAPLV
                     QKVIAAYLSDRSAGAGS"
     gene            55533..56648
                     /gene="rodA"
                     /locus_tag="PLM79_02315"
     CDS             55533..56648
                     /gene="rodA"
                     /locus_tag="PLM79_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOV85165.1"
                     /translation="MMDRRLIENFDWSIVLILLGVITVGLLSLYSALYPQIQAHPTQN
                     LFIKQLVWISLGFAGLLCILFLDYQRLRSISLWIYLSAVVMLVAVLVVGKEVNGAKRW
                     LEIVGFQFQPSEFMKIAIILHLAAYFSSQEISPYPSFKKLLYPLLVTAVPVLLVLAEP
                     DLGTAILLVTISGTVIFFMGIRLRYLITCFLLVAPMIWPIWEKVLKPYQKKRILILIR
                     PDLDPLGAGYHIRQSKIAIGSGMSWGKGFLNGTQTKLHFLPEKHTDFIFSVWAEEWGF
                     AGCLTLLVLFAALIVLSFRVARRSKDRYGSLLVVGMTSMILWQALINMGMVIGVLPVV
                     GITLPFVSYGGSSLITLCIAVGFIENVSMRRYVFQTR"
     gene            56893..57318
                     /locus_tag="PLM79_02320"
     CDS             56893..57318
                     /locus_tag="PLM79_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HOV85166.1"
                     /translation="MISINETLLFQMINVLILVFLMNLVLYRPIRRIVAQRKQLVQEQ
                     EGAIERAESEATATVQEFDARIQEARKTGRQRIQELKNEAYEYEKGLLQQATESASKQ
                     LGEVRAKVAKDIGTARDQLKKQVKALSTDLAQKILGRSL"
     gene            57346..57927
                     /gene="atpF"
                     /locus_tag="PLM79_02325"
     CDS             57346..57927
                     /gene="atpF"
                     /locus_tag="PLM79_02325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOV85167.1"
                     /translation="MKGSIRTLLLAAGLLVALGGAAWASGGGGGEGHGLNWTDFALRF
                     LNFAILVAILAKLLKKPIAGFFSSRRESIKSLLAELELKRKEAEQKTAEYKARLAALE
                     GETKKIVDELIAEGQLERQKIVEGAHRQAEYIRQQAQLAIQQEIKAARESLQEEIAEL
                     SVAAAEEILRKKMKADDQERLVREFMTQVVEAK"
     gene            57924..58475
                     /gene="atpH"
                     /locus_tag="PLM79_02330"
     CDS             57924..58475
                     /gene="atpH"
                     /locus_tag="PLM79_02330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOV85168.1"
                     /translation="MKNLVIAKRYAKALMSLAQADQAVESYGLELDQLLHLIGEQPTL
                     GDVLQNPLYPMAARKSLFQSVAKAAGISPVMTSFVNLLIEKKRVQHLPEIAQYYRKLM
                     DEHANIARAKLAAATVLAEDTIGQIAQTLEKLTGKKVIVEFEHDPALIGGVTAQIGDM
                     VLDGSVRRQLLNFKETLKRGAVG"
     gene            58476..>59257
                     /locus_tag="PLM79_02335"
     CDS             58476..>59257
                     /locus_tag="PLM79_02335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699430.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOV85169.1"
                     /translation="MEIRAEEISQIIREQIKDYEKKVELSETGRVLSVGDGIARVYGV
                     EKCMSMELLEFPTPHGTIYGLALNLEEDNVGVAIMGEDIHIKEGMEVRRTGRIAEVPV
                     GDAVLGRVVDPVGNPMDGKGPVEAAEFSRIERIAPGVIARQSVNEPMYTGLKAIDAMT
                     PIGRGQRELIIGDRQIGKTAIAVDAIINQKDSGIICIYVAVGQKKSTVAQVVETLRKH
                     GAMDYTIVVAANASDPAALQYIAPYAGCAMGEYYRDRGRHAL"
BASE COUNT        12083 a        16825 c        18208 g        12041 t
ORIGIN      
        1 cccaagttgt cgaactcccc ggcaacctcg acaacttggg tatcgggaca tggactcggg
       61 ggtgaggggc cgtttcggag ccgttcgggg ggatgtcggt cgaatcgacc aatgccgggg
      121 ggcgttcaat gaagtcgtaa ttgacgttgg attccctata cgatagtagg attagttttt
      181 ttcagacatc gcgcaaagtt cgatcgggcc cgccgggtac gggatttcgg gtgagcggga
      241 ggcgggcggg tcaggggggc aaatccgcgg cgggggcgca gcaacgggaa aatccccaag
      301 acgccgtggg gggaacggaa ccggagcctg cctgaaacga agcttgcgtt gcgacggaaa
      361 ggaaactgac tgccttgaag aaagcccttt ttctcagccc tccatccttc aagggcttcg
      421 acggagcggc aggggcacgc taccctgccc ggcgagaaat cacctcctac tggtatccca
      481 cctggctggc gcagcccgtg gccatgatcc ccgggagcag gttgatcgat gctccttccc
      541 acggactttc cttcgagagc gtccttcagt cggcaaagga atttgagctc gtcgtcatca
      601 gcacgagttc tcccaccttg aggagcgaca ttctgttcgt ggatgccctg aaggcccgga
      661 acccttcggt ggaggtcggt ttcgtcggcg cccacgtcac cgttctgccg gaagagacct
      721 tgaaacaggc cccgaacctg gatttcgtat gccgccgcga atacgattac acctgccttg
      781 agctggcaca gggggcgcct tacgagcgcg tcaagggttt gagttaccgg gccggggacg
      841 gttccgtccg ccacaatccg gaccgcgact tcgttgaaaa cctcgacgag ttgcccagcg
      901 tgttgccggt gtaccaccgg gatcttgtga tcgagaatta tttcgtcggc tacctgttgc
      961 acccgttcat ttcgttctat acgggccgcg gctgctcggc acgctgcacc ttttgccttt
     1021 ggccccagac cctgggaggt cgccggtacc gcacgagaag accggaagcg gttgtccgtg
     1081 aggtggagga gggaaaagcc ctgtttcccg gggcacggga atggttcttc gacgacgata
     1141 cctttaccgc cgacccggct cgggcggtgg aaatcagtaa gggaatgaag aggctgggtc
     1201 tcacgtggtc gtgcaatgcc cgggcgaatc tcgattatga aaccttgcgc agcctcagga
     1261 acaacggttt gcggctcctg gtcgtcgggt tcgaatccgg aaatcagcag atcctgaatc
     1321 ggatccgcaa gggggtcacc ctcgatacct cgagacggtt catgcagcat tgccgggaac
     1381 tgggcatcaa ggtgcacggc acgttcatca tcggcctgcc catcgagacc cgggagacca
     1441 tcgaaaagac gattcgcttc gcccaggagc tggacccgta caccatccag gtgtccatcg
     1501 cggccccctt cgagggtacg gatctccatg ccgaagcggt gaagaacggg tggatcgaca
     1561 acggcaccct ggtggccgaa aacgggatcc agatgtccac tctccactat cccgacctgg
     1621 acgtcgatga gatcgaggaa gccgtcgagc gcatgtaccg acgcttctat tttcgctgga
     1681 agccgattct tcggatgttt cgggaaatgg ccacggagcg gcacatgctg gtgcgacggc
     1741 tgcgcgaagg cagggaattc ctcgactatc ttcatactcg aaagaggatc ggagggcatt
     1801 tgtctgcagc gccggggact tgtgccgacg atcctcgcag gtagtccatg gatcgcccgc
     1861 ctctctatga cgaattcaag ggatgcaaag gcattctgct cgatttcggc ggcactctcg
     1921 attccgacgg cgagcactgg ctcgaccggt tctacgacct ttatgagaag gaaggggtcg
     1981 atgtgtcccg ggaggagatc aagcgggtct tctaccgtgc cgacgctttg tgctgcgggg
     2041 accccttcgt ctcgggactg ggcctcagag ccctcatgcg gcatcacgtg cgcctccagt
     2101 tggaggagct gggacgatcg gtgcccgcat gggaggagcc gctggtcgag ggtttctgct
     2161 ctcggacgga gtcgtttctc cggcgcaatg ctctgctgct ggcccgcctc agggagcgat
     2221 ttctcctggg agtggtgtcg aacttttacg gcaacgtagc tgtcctgtgt caagaggccg
     2281 ggctttcgcc gtggctggat gtaatcctgg actcgacccg ggtcggggtc tgcaagccgg
     2341 acccggggat ctttcgaatg gccctggacg gcctcgggct cgaacccgcg gaagccgtct
     2401 tcgtgggaga ctcctacgag cgcgacatga tgcccgctcg aaagatggga atgaggacca
     2461 tctggatgaa gggaccccgg ccgcgcatcc ttcgagatgc ggaaccggtg gacgggtgca
     2521 tctccaacct caccgatctg gaactgctgc tgacatgaga atgggcatca ttgccgccgg
     2581 cgccggagag cgcctcgccc ggggaggggt tgcgattccc aagcccctgg tccccatcgg
     2641 gggaaagccc ctcattgccc ggattatcga ggccgccgcc cgcatcggcc tgagttccgt
     2701 cgcctgcatc gtcaacgacc tgaccccggc aacggccgcg tacctgcggg cgacgccgtg
     2761 gcccgtgccg gtgagggtcc tggtaaggac cacgcccagt tccatggaga gcctgtttgc
     2821 cctgcgccct tttctcgagg acgggccctt cctgctctcc accgtggacg tggtcttcgg
     2881 gccggacacc ttggcgtcct ttcaccggga ggtgatcccg ctgacggagg ccgccggggt
     2941 cctggcgctc acccggttta tcgatgatga aaagcccctg tacgcggccg tggacgaggg
     3001 cgggcgagtt acggccctgg gggagcgggc ctcggggagc gaattcatca cggcgggatt
     3061 ctatgcattc cgccccgaga tcttttcgct catcgaggaa gctcgggccg gaagaatgac
     3121 ggccttgcgc cagttcctgg gattgctggt ccaaaggggc ctccccgtct acggcattcc
     3181 cgtctcgaaa acggtggacg tggattatcc ggaagacatc gccaaggcgg aggccttcct
     3241 ggaggagatc ggcgggagat gaaacgggag agcgtcctgg gcatttatcg agagcgcgtc
     3301 ttttccccgg gaaaggtccg ggacgatgcg gccatcctcg acgagaccct cgaagagctc
     3361 tcgtccatgg ggtttcgagt ggacgccgtc cggggcgaag ccttggacgg gtccgtcccc
     3421 cggccttctc gggtgctcac catggcccag tcccctcccg cgctcgccct cctggaaagc
     3481 tgggtttcac tgggaacccg ggtgatcaac tcggtggatt cgatccgaac gtgttaccga
     3541 aaacccctta gccgggcgct cgatggggcc gggctgccgg tgcccccaag caggatcgta
     3601 cccttggacg aggccctgga ggaaatcagc ctgctcccgt ccacccccgt gtggctgaaa
     3661 cggggggacg ttcacgccgt ccgggagggc gatgtggtgt cggtgagctc gagggaagag
     3721 ctttcccgtg ccctggaaca ttttcgccgt cacaaggtgg gcgaactcct ggtccagggc
     3781 cacgtggagg gccccgtggt caagttctac ggcgtcggcc ggggcgatta tttcgttgct
     3841 tttctagcct ccaccggcga ggacgtcacc cccagggtgg cggagacgct ggggccggtg
     3901 gcccaacgag ccgccggggc ggtaggactc gatgtgtatg gaggggacgc cgtcctgacg
     3961 gggaacggaa gcatcgccct catcgatttg aacgattggc ccagcttttc ccgctgccga
     4021 cgatcggccg ccaggaacat tgcactctac ctgcaaggca aggccccatg attttcactc
     4081 gaactgcgtc gaaatacggg cgcccccacg tgccggaacg agccgtgggc gacagggcaa
     4141 aggagcgata tcctcgtgca ttttgaaggt tcctatctca gaccgaacga aaccctttcc
     4201 tcccggctgt acgaggaaga acaggaattc gtgtcccccg gacttcagga ggtgagccag
     4261 ctttcgcgac tggtgatcaa ggaaggcagg ggctgccgtc tccgggacat ggaagggaag
     4321 acctacctgg atttcatggc gggggtcgcc gtgtgcagcc tcggccatgt gcatccccga
     4381 tatgttgcgg ctctcaaaga ccaactggac aaggtcaccg tgggaagctt caccacggag
     4441 aaccgggtgg agctgctgcg gctcatcgca tcccttaccc ctcccgggct ccgtcggacc
     4501 cagctctaca gcgggggcgc cgaggcggtg gaagccgcca ttcgactggc caagtcctac
     4561 acgaaaaagt tcgaggtgct cagcttctgg ggggggttcc acgggaagac gggaggagtc
     4621 ctggggctca tcggcgatgc cttcaaaaag aattggggca ccctgtaccc ggggctgcac
     4681 ctggccccct acgccgactg ctaccgatgc ccgttccgga tgacctaccc ggaatgcgga
     4741 ttgttctgtc tcgagttcgt ccgcaagatc cttgaaaaca atactgcggg gagcctggcc
     4801 gccatcatcg tggaacccat gcaggggacg gccgggaacg tgattccccc tcccgagttt
     4861 gtgcccgggc tcctccagat cgcccgggaa aacgacgccc tcctcatcgc cgacgaaatg
     4921 atcaccgggt tcgggcgtac cgggaggatg ttcggatccg atcacatcga tctcatcccc
     4981 gacatcatga ccatcgggaa aggcctggga agcggatttc ccgtctcggg gctcatctcc
     5041 accgacgaga tcaccgccag caagccgttc tccaagccca gttcttcgtc cagcagctat
     5101 gggggaaacc ccctggcaag cacggccgcc cttgccacgg tgcggaccat cctcgatgag
     5161 tctctggtgg agaattcacg gcgggtggga gaacacctgc tcaacggcct ccgggggctt
     5221 caggaaaaat acgagtttat cggcgatgtg cgcggcgtgg gcctgctcat cggggtcgag
     5281 ctggtcaagg accgcaagac ccgggaaccc ctggatcggt ccgtgacccg gcaaatgttt
     5341 ctcgagaccg tcaaacgggg gatgatctcc atgaattaca agcccaactt ccgcatcaat
     5401 cctcctctgg tccttacccg ggaggaagcc gacgaggggt tggcgatcct cgatgaagtc
     5461 ttccggtttg tggccgatca cgtcccgtac aagtaagcgt cggaagagga ggagtcggac
     5521 atgggaaagg gaaatgcccg gatcgatcgg cctgcggcag actggggaag acggccctcg
     5581 atcgaacagg gctccggaac ggtttgaaat ggtcttggat atatcgaagg acgggaattt
     5641 gctgcgaggg gccatcatcg gttttggaaa cgtggcggag aatgcgcacc tgcccgtatg
     5701 gcgcaggagt tctcagttca ggatcgaggc cgtggtggaa cccgatcccc aaagggcccg
     5761 caaggcccgg gaattgctgc cgggggtgcg ggttcacccc agcgcggagg agctgttccg
     5821 ccaagacggg ctcgattttg tggacatatg cactcctccc cgtttccacg acgacctggt
     5881 ggtggcgtcc tgccgggccg gcctccatgt cttttgcgaa aagcccctga cgacctccac
     5941 gggcaatttc gaacgcatcc gggaagcagc ctcgagggcc cggaaggtgg tgtttacggt
     6001 aaataactgg aaacatgctc ctctctgggc aaagaccctg gaattgatcc gggcgaatga
     6061 aatcggcgag gtacgaaccg tttccatgaa cgtccttcgc gtctcgagtt ccggaggcgg
     6121 agcgtccaac tggcgcagcg acccggaact ggcccggggg gggatcatga tcgatcacgg
     6181 ctggcacaac ctgtacctga tctttggcat ggttcgggag tccccggttt ccgtctcggc
     6241 ccggatggaa gctccatcgg gccgcgattc cggactcgaa gagacggtgg acgtggtgat
     6301 ccgttttccg cgggccgagg cgcgccttca ccttacctgg tgcgggccct gtcggcgcaa
     6361 tttcgggacg gttgtggggg aacacggcac cctgtccatc aacgacgacc atatcctggt
     6421 ggcctcgaac gggtccgcgc cggtgcgcta cggcttcccc gaggcgctgt ccgccgggtc
     6481 tcatcatccg gaatggatgg atcgcgtggt cgaggatttc gcccgggaaa tctcggacgt
     6541 ccggcgacgg ggcaccaatc tcatggaggc ccgccgatgc attcagctca tcgaatcggc
     6601 ctaccggtcg agccgggagg gctcatgccc catcggactc cgcgacccgg cgggttgacg
     6661 gcggcagggt gcatgcagga aggtcgccgg tttcgtacgg ccgggccgcc cgccgacgga
     6721 gatggaaacg gcatgggggc gtcgggttca aaaagagagg ggatcgcttc ggatgataaa
     6781 gttccacacg gtgtgtgtcc ttctgggcct ggggctcttg gtctttctga tccgacagat
     6841 cggttttcag gagctgtgga gccagatgag cctgctgggc tggggcctgg ttccgctagt
     6901 gctgatcgag gtcgtggcgg atgccctgca caccatcggg tggaagcatt gcctcccgag
     6961 aacgctcagg tccctttcct tctcccgcct ctttcggatc cgcctggcgg gctatgccat
     7021 caactacctg acccctacgg cgaccttggg cggtgaaatc accaagggaa tgcttctttc
     7081 cttgaaccac accgccacgg gggccttcca gggcgtcgtg gtgggaaagc tcgcctacac
     7141 catcgcacaa ctgctgtttg tctccctggg gtccatcctc acgctctggg gggtggatct
     7201 cccccgcggt gtgtggcctg ctttgctctc gggcagcgca ctcctggctg ccggggtcct
     7261 gggttttctt gtcgcccaga aattcggcaa attgggtgcc gttcttcgct ggttgtccgg
     7321 gcgaaaaagg ttgggcaaac acctcgctgc ggccgccgcc aaggccaccg aagcggatga
     7381 ggagcttcgg ctcttctacc gaagccaccc cctggagctg cccattgccg tcctgtggca
     7441 catggcgggg atggcatgcg gcgttctgca ggcttggtac ttcttgtacc tactgagcgg
     7501 tcacccgtcg atgcacctgg ctgccgggat ctggtgcctg ggcgggtggt tcgatctgat
     7561 gacctttgcc gtgcctctgg ggatcgggat tcaggaggcc acacgggtct ttgccctgaa
     7621 ggccgtcggc ttcgacgccg ccatgggcct ggcatacggg gtgaccctga ggctcgagca
     7681 aatcctgcga tcgggcctgg gcctactgtg ttacgcgacg ttactgtcgg aaaaaaacgg
     7741 cctggaagtg gacgcagctc cggccaaggg gccgaattag cggttctccg gcaccggccg
     7801 cgggccttgg aagaccccgg aagaccccgg ttggctcaac ggatgttggg ggctgccccc
     7861 ggggccgctc tccgtccttc ccggtagcgg atgatccaaa gggccgcggc aaatacgtag
     7921 gaccccaacg cgctgccgat gagaaaccag ttgagtcggt ccaccagggc caggagggcg
     7981 atgagatagg cgaaatcccg gttgctcagc agggccatga accgaatgac gttgggagag
     8041 gccttgagct gatcctcgct caatcggaga atgcattggt acacggcaag aagacacaga
     8101 ccgaacccgc ccagcatgac ccacagaatt cgtaggtacc gggcattgcc ggtatcgtga
     8161 tagaggccga aggcgattcc cacgaagatg gccaaatgaa caaggttgtc cgtggtgatg
     8221 tccaggtaat ggccgaaccg gctctcctgt agtttgaggc gggcaagctc cccgtcgact
     8281 ccgtccacga tcacgcagaa cacgaagagc agcgagccca gcaggtgagg caggtaaccg
     8341 ggaagcgaca gcagaaaggc ggccgtcagc ccgatggtca tgccgacgag ggttacgccg
     8401 ttgggggtca tgggggttcg agcgagtcga ctactgattc ttcgggagat gtgccgatca
     8461 aagtggcggg cgatgaagcc gtcgtccgca tgagtttgca ggcccagggc ggccgccagg
     8521 gcatcctcgg aggcgtccgg ggtgttccct gcgccgtcca gagtgcgggg tatcggacca
     8581 cgagcccgga tgtgcctcat cccccgagct gcattgtcgt cgagctcttc tccctgccac
     8641 agtaaccgga cgacgggcaa gagccgggat cggtcgacca ggaatatccc ttcctcgagt
     8701 ctttcagtgc ccgccgccgt caggcatgca ggggcatccc ccggggtgct ctgccggaaa
     8761 aaccggaggg tatgccggtc cacgacgtga tccgctctca aaaccagcac cctttcatcc
     8821 cgggaaagct cgattcgttc gatggccgcc tcgggcgatt cgccgtcctc cagttggtgc
     8881 agaaaggccg aaggcaaaag gtccgagacg actttcacca ggggggcgac acgtccaacc
     8941 aggtggattt ctccaatgcc cagttgcttt aggatgacaa ccagccttcg gagggcggga
     9001 acgccgaaca gtttttggac gcccgcgccg tccggggcca ggatgatcgc agtcgcaatc
     9061 atgcacacgc tcctttcttc ccggtacggg taaggaggga acggtagcaa gcaggccggg
     9121 gagcgtcaag ggaaatcggc cggaggcaag gatccgcccg cagggtcctg ttcgcgtgcc
     9181 ccagtagcgc ggagtcccgc aggaaggggt cggggtaaat tagtgtctac gacgatcggg
     9241 gagtgtgagt cccgcagggc gtttacgggg aggtgctccg gacgtagtcg acgagcaggc
     9301 gggcaaacag gcggactccc acatccagag cgcgttcatc catgtcaaac aaaggggaat
     9361 gcagcccgtg gcaaaccgtt gctcccggga cggaacagcc caggcgaacc aggaccccgg
     9421 gtattctctc caggaaataa gcaaagtctt cggctcccat tcggggcggt tccacatgca
     9481 cacggtcggg tcccagtaat cctcgggcag tctcgagggt gaaccgggtc agcctctcgt
     9541 cgttgataag gatcggatat ccgggagtga ctcgaagggt gccggaaacg ccgtgggcgg
     9601 accgggtccc ctcgaggacc tcctccattc ttgtgaggac ggtttcgcgc acctggggat
     9661 cgagagtcct cagggtcccg tggagtatgg cctcgctggg gatgatgttg gaagccgaac
     9721 ccgcatgaaa gataccgatg gtgatcaccg ccgcgtccgt ggggcgaatg ttacggctga
     9781 caatgctctg cagctgcgtg accaggtagg ccccggccac gatggggtcc gcgcactggt
     9841 ggggatgggc gccgtgtcct ccctttcctt cgagtcggat atggaaactg tcggaggcgg
     9901 cgttgctgac tcccatggca agacccactt ctcccgcggg aagatcgggg tgcatgtgag
     9961 ccgcgaagat ggcctccacc gtttcaccct cgagggcccc atccttgatc atggacaggg
    10021 ctccggcccc gccttcttcc gcaggctgaa agagcagaag gacctttccg cttccctggt
    10081 cgacccattc ttcctgctcg aggagccacc gggcgacacc caggaccgtg gtcatgtgcc
    10141 cgtcgtgtcc gcaggcgtgc atgattcccg ggttccggga acgatagggg acctcgttcc
    10201 gctcctccag gggtagagcg tccatgtccg ccctcagggc cagggtgggt cccttgttgc
    10261 gggctttaag ttccgccacc accccggtgc ctcccacgcc ggttcgatga ggaatcccca
    10321 gttcatggag cacccgggag acgaccgcgg cggtgcggac ctcctgatag gccggctccg
    10381 gccattgatg gagctcgcgt cgaagggcga caagccatga atgcaatggg gtctggagcg
    10441 ggtccggcat ggaaatggcc ccctggaaag cgacaaaccc gcttttccag gatggcaagc
    10501 gggtttgcgg aaatccgtga tcgggtttcg aggttgtgcc gggcaagcat ccccttcgac
    10561 cgcttccacg ggtcgacgga ccgagaaagg aggcttgccc cgagttttct ttctactggg
    10621 gaatgttccc gtgcttcttg ggcggcaggg cttcccgctt gttggacagc atctcgaaga
    10681 tttcgatcaa acgcttccga gtctcggacg gaacaatcac cccgtcgata taacctcggg
    10741 acgcggcgat atacggattg tagaggagct cctggtagtt ggcgatgacc tctttgcgct
    10801 tggcaaccgg gtccgcagct tccttgattt cccttcggaa gatgacgttt gcggcgcctt
    10861 cggaccccat cacggcgatt tcagcggagg gccacgccag ggagatgtcg gcacccaggt
    10921 cccgggaaca catggccagg tacgatccgc cgtaatcctt gcgggtcatc agggtcacct
    10981 tgggcaccgt ggcttcggaa tagcaccaca gcagcttggc tccgtggcga atgatgccgc
    11041 cccattcctg ttgggtcccg ggcagataac ccgggacgtc ggcgatggtc aggaggggaa
    11101 tgttgaatgc atcgcaacag cggatgaacc gagtggcctt gtccgaagaa tcgcaatcca
    11161 ggcagcccgc catcaccctc ggctggttgg cgatgatgcc gatggaacgg ccgttcaacc
    11221 gtgcgtgtcc cacgataatg ttcctggcgt agtgctcatg aatctcgaag aattctcctg
    11281 cgtccacaat ggaccggatg acatccttca tgtcgtaggc gcccatgggg ttgtcgggaa
    11341 tgatggaatc cagctccgga cacatccggt tgggatcgtc tcccatgtcc acgcagggag
    11401 gatcctccat gttgttgtcc gggtagtagc tgagaagttt cttcatctga tcgatggcat
    11461 cggcgtcgct ctcgcaagcg aagtggcaca cgccgctctt gctggcatgg gccatggcgc
    11521 cgcccagctc ttcctgggtg atgatctcac cgagaaccgc cttgatgacg tcgggacccg
    11581 tgatgaacat gtaactgctt cccttgacca tgaaaatata gtcggtcatg gcaggagaat
    11641 acacagcgcc cccggcacag ggtcccatga tggcggagac ctgcgggatc acgcccgagg
    11701 agatggcatt tcgatagaag atctgaccgt aaccggagag tgaatcgacc ccttcctgga
    11761 tccgggcgcc gcccgagtcg ttgaatccta cgaaaggctt gcccgccttc aaagccatgt
    11821 ccatgacctt gcagatcttt ttggcgtgca tttcacccag gcttcccgcc cgagccgtaa
    11881 aatcctgcgc aaaagcaaag acctttcggc cattgacgtt gccgaacccg gtgacaacgc
    11941 cgtccgccgg aatttccact ttatcgatgc cgaacagagt tcctcggtgt ttcacaaaca
    12001 tgtcgatttc gcggaagcta ccagggtcga aaaagtaatc gaggcgttcc cgtgcggtca
    12061 ttttgcctcg ttcgtgctgt tgtgcaactg cttttggacc gcccatctcc ttgatctttg
    12121 cttcccgctc gcgcagctct tgaatcctct cagcaacagt cttcatcttc gatcctcctc
    12181 cggttctata atttgagaga ctcctggttt gcttccagca tacaatgttt gagctattct
    12241 aacggttcat gaagggttgt caagcgcttt cccaaccccg cggatatccc tnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttctaacgg ttcatgaagg gttgtcaagc
    12421 gctttcccaa ccccgcggat atccctgcat tccgggtttc cactctctat gcaagggtcc
    12481 tttcccttgg caggttcgcg gtcgtcaaag gcgcccgggt gcctgaaaac gtggggcgtt
    12541 gcggctcgcc acggttgatt ccacggacgg ggctgatgtg gaaggaagat aatcaaggac
    12601 acaataatgg aagcggtggg aaaaatcgac caatattttt ctcgggttcg ggaagaaggc
    12661 gtccgattcg ttccggcggg cgacttgacg cgccggcgcc gggctgccgg gaaacaagac
    12721 tccgttcctg tagggccctt cgagaagccg gggtctctcg ggctatttcc ggcaggttct
    12781 gggagggcgg atgggaaccc atgctccacg aaggttggtc ccgaccggcg ggacggtcga
    12841 cgttcaagga ggaccggaga gcggcgaaac gcctgcacct tggggaatat ggcgaacggt
    12901 gtggccttgt tccctggccc actgccacaa ggcacggcta tgctcccggc gttgggcgtc
    12961 gtccaggagg acaaaatgga gggccagggg aaagcggccc aggaactccc gggaaacggg
    13021 tccgtcgcag accaccacgt ggtagccttc cagggggacc tctcctgccg tgacggcaac
    13081 ggcttccctc ccgggaggtt ggttcgggga ggagacgatg cggtggggta caaagcttgc
    13141 ctgggaaaag gtccacagca actggtccag ttgttgggca ccgagggtcc cgtcggtgac
    13201 cacctgtacc tttcggcctt cctcgtagaa atgttccacc cagcggcata agagccgttt
    13261 ctgctcctcg ctccgggttt caacaaaata gatgtgcatc gtcggcatgt cctgtcagca
    13321 tgtcaggtga aaggccccgg ccacattcct gccctcgcgg gtcagccgat tgaaggattc
    13381 gaccgctttg gaggtgggct cttccagcag ttggatgttt gcctggacca acctggaacg
    13441 gagggactcg aggacttgca ttctgccggg ttctcccatg cccaccacca ggatttccgg
    13501 ttgggccgaa agaatgtcct cgacatcttc gggcgcaacc tcgtggcctt ccttgcgcca
    13561 ccaatcgggc aataccttgc ctcccaggat cttgaggtcg cttcggtacg ctttgccttc
    13621 aatgaggatt cttccgaatt ggtatcccga gatcacaaaa ggctcctatc gtttcgaatg
    13681 gaacttcgat ccgggtccgg ggcgacgggc cgaccggccg ccagcccggg aaacgccggt
    13741 cctctggacg agagtgcgcc cccccagggg gtgaaacgat tctcggatcg ggtcgtccgg
    13801 gctcaatgga cggttgtctt atagaccact tccgcgcaat cggacccaaa ctgctgggca
    13861 atctcctcga agaagccgcg tgcgggattg acgctcagct tgggattgag agcgatgacg
    13921 gcctcggcct tggaatccac caccaggtga agaaaggttt tgcagtcgcc cgcatggctc
    13981 atgagcaggt gcctcagccg ggaaagggca tccttgtcga ggtcttcggc acggaggcgg
    14041 attcggacgg catccacgga ttcgagctga gcttcggcca gggtaaggat ccggttggcc
    14101 accaatttcg tccctttttc atcgtgttga acctttccca gaacgacgag gggctcatct
    14161 ttgtcgagca gggggcgggc ctgcaggaac gcttccgaga aggccaccac ttcgaggatt
    14221 ccctccttgt cctccaggga gagaaacgcc atgcgatctc cccgcttggt ggtcaactcc
    14281 ttggtgatgg tgaccatacc gcaaagggcg acctcgaccc cttccctctt ctcactgacg
    14341 ctctgggtat cggcggtgca gagccgggcg atccgttccg cgtagaaatc caggggatga
    14401 ccggaaatgt agaagcccag ggcctccttt tcgaattgga gggtggtgcg gttatcccag
    14461 ggttccaccg tgggcagagc cggggtcttg cctttttgac ttcgaagcag ctcgaacatg
    14521 ttcatctgac ccgtctggcg gtctctctgc accacctggg ccttttccag ggcgtcgtcg
    14581 gcggccgcca gcacctgggc ccggttgccg tggatggtgt cgaaggcgcc gcacttgatg
    14641 agttgctcca ggaccctccg attcaccttc tgggtatcca cccgccggca gaaatcatag
    14701 atggaccgga agtgcccgtt ttgatcccgc acggtaagga tggactcgat ggcggtctct
    14761 cccacattct tcacagcccc caggccgaag cggatcttcc cctcgacgac ggtgaagtcc
    14821 ttgctgctgg cgttcacgtc gggcggaaga atctcgattt ggttttcgcg acactggttg
    14881 atgagcttca ccacctggtc ggaattgctg agaaagctat tgagaagggc cgccatgaat
    14941 tctacgggaa agtgggcctt gagataggcg gtttgatagg cgatgagggc ataggcggcg
    15001 ctgtgagatt tgttgaatcc gtatccggca aacttctcca tgaggtcgaa aatgtgattg
    15061 gctttggcca gatcgatgga gttctcctta gctcccgaaa tgaatcgatc ccgttgggcc
    15121 ttcatcacgt cggggatctt ctttcccatg gcccgccgca gaatgtccgc ttccccgagg
    15181 gtgtaattgg ccaggacgct ggcgattttc atgacctgtt cctggtagag gatgacgccg
    15241 tgggtcggtt cgagaatggg gcgcagcatc tcgagatcgt aggtgaccgg agccaggccg
    15301 tgtttgccgt cgatatactg gtccaccatg ccgctctcga gaggccccgg tcgataaagg
    15361 gctaccaggg cgacaatgtc ggcgaaattc tcggggcgaa ggcgaaccag gatgtccctc
    15421 attcccgaac tttccagctg gaataccccc gttgtgtcgg cgcggctcag gagcgcatag
    15481 gtttcgggat cgtccagggg aagggcctcg gggcggatgt ccaggtggtg gtttttgcgg
    15541 atcagttgaa cggcgttgtg gatgaccgta aggtttctca aacccaggaa atcgaacttg
    15601 atgagacctg ccctttccac gtatttcatg ggaaactggg tgacggtttc cccgtcttgc
    15661 ccccggtaga gcggcatgta ctcgacgatg ggcttgtcgg cgatgaccac acccgccgcg
    15721 tgggtggatg cgtgtcgagt gagtccctcc agggcaaggg caacttcgaa gagttccttg
    15781 atctggggat cgtttcgctg ctgctccccc agccggggct ccgcctgaaa ggccttctgc
    15841 agggtcatcc ccggggatgc cgggatcatc ttggcgatcc ggtccacctc gttgtagggc
    15901 atggccaagg cccgtcccac gtcccggatg gccgcccgcg cctggagggt gccgaaggtg
    15961 gtgatctggg ccacccggtc ctgcccgtat ttctccgtaa cgtaccggag cacttcctct
    16021 ctgccgtgga cgcagaagtc cacgtcaatg tcgggcatgc tgattcgctc gaggttcagg
    16081 aaccgttcga aaatgagccc gtgctcgatg ggatccagat ccgtgatctt catcacgaag
    16141 gccaccaggc tcccggcggc ggaacctctg cccggtccca cggggattcc cttctgcttg
    16201 gcgtagttga tgaaatcggc gacgatgaga aagtaggcca cgaatcccat ccggcggatg
    16261 acgtccattt ccaggcgcag ccgccggcgg tactcctgct gatccttgtc cgtgaaatcg
    16321 ggccgagtcg agcggatttg accgatcctc tcctcaaagc cttccatgac cgtcttctca
    16381 aaacggtcct cgagagtctc tcccggttcc agggggaagt ccgggaagtg gtactctccc
    16441 agctgcagat ccaggttgca ccgctcggcg atcacgaggg tgttttccaa ggcttcgggc
    16501 acgtgggaaa aagcgcgggc catttcctcg ggggacttga aatagaggtg gtccgtctgg
    16561 aacttcatgc gcttttcgtc caggatggtc ttccccgtct ggatgcacag aagcacttcg
    16621 tgggcacggg catccgcctt gcgcaggtag tgacagtcgt tggtcgccgc caggggaatc
    16681 cccaggtcct ttcccatgcg gatcaaggtt tcattgaccg catcctgttc cggtatgccg
    16741 tttgcctgaa gctccaggta gaaattctcc ggcccgaata tctccgcata ttccagggcc
    16801 gtctttcgtg cggtgccgtc ctggtttcgc agaagcaggg aggcaatctc tcccttgagg
    16861 caactcgaaa gaccgatcag gccttcgtgg tattgcctca gcagggcctt gtcgattcgg
    16921 ggcttgtagt aaaagccttc gagatacgcc cgggtgacca gcttgagcag gttccgatat
    16981 ccggtcaggt ccttggccag caacaccagg tggtagttgc ggtcctcttc cccgttggcg
    17041 gtgagctccc cgccggtgtg gcggtccaaa cgactccggg gagcgagata gacctcgcac
    17101 ccgatgatgg gcttgatccc ggctttccgg gccatgtcgt agaactcgag ggcgccgaac
    17161 atgttgccgt ggtcggtgat ggcgacggca ggcatctggt agccgaccgc cgtctccagg
    17221 agatctttca ggcgaattgc tccgtccagc agactgtact gggtgtggac gtgaagatga
    17281 acaaagggat tcatgggcag cctttcctcc cgaggagggt tttcgcggat ggaatccaaa
    17341 agtggaatgg acctcgaaag gcagtaaaac atttgccgat gaaatctgtc aattggtcct
    17401 ccctggattt ttcccgggcc tgattccgga tctcggccgc atggtgccaa aatccctgcc
    17461 gcccgtgtcg ttgcgacttg attcaaagac ggattctcct ataattcacg ggaacgtcct
    17521 tccggggggg cgggccaatc tgcgcagggt cgtctcctcc cgggccgggg tgcgaagata
    17581 cgatcctatt tcaggagagc gagggatgcc gaaattgccg gggagaatgc tggggttggc
    17641 tgtgggattt ctccttttgt ggagtgttcc gggtgattcc agccccctgc cttccgcccc
    17701 caagaaaaac tccggccaga attccgatct ggtgcatcaa gtcaaggccg gtgccctgtt
    17761 cgactatgcg aagcaggaaa acaagcggct ccgctgggac gaatgtctgg cccgaaaggc
    17821 gctggttcga gcccgtaaga tggtgcagga cggatatttt tcccaccagg accccaagac
    17881 cggcaagaac ccggcctgga aatacgtcac ggcctgccac aaggtccgtt atgccgggga
    17941 aaacctggcc atgggatatg aatcggccaa gacgcttcat gaggccctca tggatagtcc
    18001 cacccaccgg aagaacatcg tgagccgccg gcacacactc ttgggggtgg gctgttacga
    18061 ttacatttgc gtccagttgt ttgccgggtt ttgacggggc tgaacccggt cccgtttgga
    18121 tcgcagcacc ctgagacgct gtcggcccac ggcggggaag gccgccgatc cccggggaat
    18181 tctcttgatg tttctcggga cctgtgttag gcaaaatacc taccggcgcc ggattggcgc
    18241 ttcgacatgg tgggtgtagc tcaatggtta gagcactggg ttgtggcccc agaggctgtg
    18301 ggttcgagtc ccatcactca ccccatcccc ctaatcttcc tttataggtt ttcccaagca
    18361 gagaggacgg gtggagtttt gtggcttcgc cccgggattc ggcccatcga agcgggttcc
    18421 ggtggggagc cgcccggctt tgagggcccc atccggggcg aggaacggcg gtcgttgacc
    18481 gagaaggccc cgggctcgag cgcacagaat gcggatggaa aggttccggg cccggcactg
    18541 cggagacagg ccgccgccgg aatcgggaag accggatgac gcgggagtcg ccgcaccgca
    18601 agagagagcc gttctccacg aggcccggca agggttgcca tagggatcgg ggcaggcccg
    18661 ctgatccgtg acagacggct cgaaccaccg gaacgaaaca aacatgagga ggctgcgatg
    18721 aaatgggagg caaaaggaat ctccgcaatt ctgttggcct tgatcctggg catgtccatg
    18781 ggtgcgcttt tccagggtga ccgtgcagat gccgcggaat cgaagctctt gcagggcgag
    18841 gaatgggcca agatgtccct ggagagcaaa cgggcgttca ttctgggtgt gggggagatg
    18901 gccgacatcg aggaggagat gatcggcaag tatcccgagc tgaagcggga tagcttttcg
    18961 gaaaaggtgg cggaggcttt gggagatgtc ccgctggata ccgtcgtgaa ccggatcgat
    19021 gccttctaca aagcgaatcc ggccaaaatg aacgttccgg tcatgcgagt ggtctgggac
    19081 gagatgatca aacccaaggt gtcgaccatc gcaggaaagc cggtgaagaa ataggcggtg
    19141 ggatcccctc ggccggaaat cccgcagctt tgcgtctata gatgaatgcg gtgaaaatca
    19201 aaccgaaaac agggagtgcc cccatgaaaa gaaacagaat catggcattg atgcttgtgg
    19261 tggcgtgtct gttcaccttc gggtgtgccg gaatgagcga aactcaacag cggaccctca
    19321 gcggcgcggc catcggagcc gcgggggggg cggccctcac agccattgcc ggagggaatc
    19381 ccgccgttgg agccggtgtc ggcggggcgg tgggaggact cacgggatac attatcgggg
    19441 agcagcataa gtagcctgct tcatgggcaa cggcacgttc cgtcttcctg agccgatccc
    19501 ggagaacggg agggggtgag ggaaggatga aatccggcat cggtccctcc tctcccgctc
    19561 cgggggcgaa cccgcgggga aagagttcat cgcccggccc cccgggttct tttgcgaagc
    19621 aggccgaggg ccgagtggta tctcgggtcc gggtacggcc cccaagagct ggggtgcgcc
    19681 ccggtgagtc cgggggaaac cgccaatgcg gccgccccct ggtccggaac aggcccgcat
    19741 tccgaaagag agctccgacc atacggcatt tctcggcgat cctgaattgc gaggacctgt
    19801 agggcagcac tccgcagccg cgtctgttcc cggcaatggg acggcttggg cgagggcatg
    19861 attcggcatc atcctccggc gatgattcgc tccacgtccc gaatgagttc ctcgttcagg
    19921 atctttcgcc tcttcatatc ttcggcggtg aacagggcgt aggaacccaa ggacttggcg
    19981 cacaattccc cggcatcgtt ccagaggaac ccctccatcc acacctcccg gtccccccga
    20041 cgttccagaa tatgactttc caccttgagc tcccggttca cataggcggg cttcaggaag
    20101 tctacggtca tggatttcgt gagggtgatt ctgcccacaa ggtgcagcac gccccagccc
    20161 atggcttcgt ccaacatggt ggccaggatt cccccgtgaa cgagttggtc ccatccgctg
    20221 agattaggag cgactcgaac ccgggacacg atcttctgcc cgtcggtgta gaaattcatc
    20281 ttgagtcccg acgggttggt gcggctgcag gcaaaacaat tgtcgctttc cctgtgggga
    20341 aggagccgat atttttcttc agacattccg gagtttctcc tttccggttt ccggtcacgg
    20401 gcgacggact cggcccagag gacacgaaag acgaacccgg tctgcaactc ggggctcagg
    20461 cttcctcatt gtccgcttcc tcttccaggc gcaagagcac ctcgtacatg aattcgagga
    20521 aatcgtgctg gatttggagc cgttcgatgg gatcgctgga agcggctgcg gcagccagga
    20581 cgacgagctt cggctcttca ccggactcct ctccttcctc ctcggaggtc tcgatccgga
    20641 cggtaccgtc gttcattcct tcgatgatct ctttaagctt cagattctct tcgtccaggg
    20701 aaaaggcaat gtgtggttca aactcggctc tccaaaggtg cccggcacag gaataccggg
    20761 tccagtggcc gagggaccaa aagcgggtga gcaggtcggc gagcagatcg tccaggtcca
    20821 caaggtagcc gtcgcagtcc ttcacggggc agaccaggtc accgtcctta tccggtttga
    20881 ccagattcga ttcaaagaaa ttggaacagt cggtacatgc aagcatgggg tcgtccttga
    20941 tcctgtaatg ttcgtgccga ttgggattcg gaaaggtccg gcggtatccg gcggtgcagg
    21001 ccgaaccggc gatccgtcta atatgcggtg tcttcctcgt ttttctgggg caatggagag
    21061 ggctccatct cccttccgcg gatttccagg cgcttgagtt ttcccttttc gttcaatacg
    21121 cgctccatct ccttgcgcac ccccgcctgg ttgatgaggt cggagagaag gattcgctta
    21181 tagctgatgt gcctgagttc gttttccagg tactggtctc tttcctgttt cagcttcagg
    21241 ttctgcataa agagcctttc ctcgagggtc tcattctcca cgagcagcga caagagcata
    21301 tcggtgagct tgctcttccg gagacccatc atgccgatgc ctgcggcttt tctgcggtac
    21361 tgatccaagc ggtcctccag gaactggatg cggcgttcga gctcggcctt ctccgcttcg
    21421 agctgttcct tttcgtggtc ccaaaaacgt ttcaaggccc gctcgatggt caacatctcc
    21481 tcccggtcgt ttgcactcaa cgcctggtac ttgcgcacgt tttttcggta ccccaggtaa
    21541 taacccagag acaggagggt cacgagcact aacagggctg ccattagttg gtacatcatg
    21601 atcaggttca tgggatggtt gcgctcctct ctgctccgcg ggcggagcaa tttgcccctc
    21661 gagaaggctg gagaaatctc ccgttatccg tcggcttcgg gcaccgagaa gatgcgttca
    21721 ccccgagaaa gaacggggta gcttctccca tgcccggccg taaatttttg cccattatag
    21781 ttcatttgac gtgggtttca aaatggaagg atggaggtcg acctgggaaa cgttcggggg
    21841 ggcctgtgca cgagaacgag gttgacgctg cgcgccttct tttgctagtg tgatcaacgc
    21901 ctcgcgtaga tctgaacgcg gagggggacg cagggctttg tccatagtct agagaaagat
    21961 tcattcttct cattttggga tacggtctca ctttaccact ccgaaagttg tcaatcacca
    22021 gggagtccgt tgactgcttt ggagccctag attcatcatt ttcccatcgt aatatcgtaa
    22081 tagaggaatc cttaatcttt ttcggttcac ggcaaaacag cagccgcaat tttggggtgg
    22141 cggggagttc gctcgacgaa cggaaacggc ttggatctct tggaacgagg cggccatgat
    22201 gctggttcag aggatcaagt tggaagatct ggtgtgcatg gaggaccccg aggatgtctt
    22261 tgaggaatgc aagaggctcc ttttcctcat gttcccgaac tactcgcagc agccccttga
    22321 gaaggctttc gaacacgtcc ggggactttt cctgggcaga tgccctgggt acttccggtg
    22381 caacacccgc taccatgact tgaagcacac tacggattgc ctgatggctt tgattcgcct
    22441 gatccacggg gcgcatcgga cgggggtcca aatggaggag ggtatggtca ccctggccct
    22501 catcacggca ttgatgcacg atacggggta cgtccagacg gcggacgatg tctccggaac
    22561 cggcgccaag tataccctca accacatcga gcgcagtatt tgctttatga aggaatactt
    22621 cgatgcacag gggtattcgg cacaagaatt tgccgcttgc cgcgatatcc ttcgatgcac
    22681 cggactggat gtgcagatga ccgagattcg atttcagtcc ggggagcacg agatgttggg
    22741 catgatgttg ggggcctcgg acctgcttgg ccaaatggcg gacaggacct acctggagag
    22801 gttgcctttc ctgttccagg aatttaagga gggcggcgtc cccgggttcg aaagtgagtt
    22861 ggatctgctt cggaagaccc cgggcttttg ggagtttacc aagaagaggt ttgaaaccca
    22921 gctgggaaat gtggaccgat acatgcttca tcatttcctg actcgttggg gcatcgactc
    22981 ggacctctac cgggaagcca tagaaaacaa catatcctac ctcaaataca tcttggaaca
    23041 gcatcccgac aattacagcg actatcttcg ccgaggcggc ctgatcaagc tgctccggga
    23101 aaggcaattc cgcgcggaca cccgagggga ggcgtgtaaa ggcgaggagt agggggcgac
    23161 cgcgaggcag ggccgttagg acggacggat acagaggcgg ccttcgcaag cccaaccccg
    23221 gccgccgggg ggaaggagga gggtgactcg tgcggcgata cggcttcagg aagacttcta
    23281 catctccctg ttgacaggcc ctgttgggtc cgtgggaaac aaagttgccg tgcggggttc
    23341 actgtgcttg acaaaatcct aattttgata tacataactc ctaccttttt tcctgaggat
    23401 tggagcgaat ggggcgtggg gaggagcgtg ccgccgggag cgagattggc ggaaaggcca
    23461 cggccccttt gccgacacag ttgctgggat gggtgagtgg ggttgagtcg gtaatgaaac
    23521 taaggtggag aatgagaggc atacgagaga cccttgctga ggtccccgag ggagatgacg
    23581 agcgggagaa tttccggatt gttttgaagg gctcggagca tcccctggat gagaagactt
    23641 tttcccacct gaggccaagg gtcgagaaag gaagggaacc cgtcccccac cattcgacga
    23701 gacattcgag tctccagagc tcctggatcc gttccatcga taaggccatt cagagtacga
    23761 ctcagttttt cctcagaacc cagtaccctg agggcttctg gtggtccgaa ctcgagtcca
    23821 acgtcaccat caccgccgaa tacgtcatgc tcctgtattt gctgggccgc ctggacccgt
    23881 ccagaggcag gaggatggcc gaatacctgc tcaacaagca gagccccaat gggtcctggc
    23941 ccctttatta cggggacgag ggcaacctga gcatcactgt ggaagcctat tttgccctca
    24001 agctgttggg agagaaatcg gattcggaac ctctccgaag ggcccggtcg ttcatcctaa
    24061 acaatggcgg actggaagcc agccgggtgt ttaccaagat ttggctggct ctattctccc
    24121 aatacgattg ggacaaggtc ccttccatgc ccgtggaatt ggtcctgctc ccttcgcatt
    24181 tttacttcag catctacgaa ttttcgagtt gggcgaggtc cactgcggtt cccctctcca
    24241 ttgtcctgtg cattcgaccg acccaccgtc ttcccaggga gaaatcggtc tcggagctgc
    24301 atccgacacc ggggtccgcg acccgcaaaa agcgcttcgc atccatgacg cacaagcttt
    24361 tcttcctctt tgaccgggtg gccaagtggc tggaaaagca cccccttccg gaccttcgtc
    24421 ggcgcgccat ccggaccgcg gagacttgga tcctggagca tcaggaagaa acaggagact
    24481 ggggagggat ccagcccccc atggtttact ccatcctggc cctccattac ctggggtatc
    24541 ccctgaatca ccccgtgctg gtcaagggga tgagagccct cgatgacttt tgcatcgagg
    24601 atgacttggg ctatcggatg caatcctgca tttcccccgt gtgggacacg gccctcaccg
    24661 cacttgccct catggaatcc ggaatgccgc caactcaccc gaccctgcaa agggtacagc
    24721 agtggctcat tcagagacag gtgaagacgg ggggagactg gcaggtgaaa aactgctgcc
    24781 cgccgggagg atgggccttc gaattcgtga atacccagta tcccgacgtg gacgattccg
    24841 cggtggtgct cagcgctctc catcgcctgg gcagcactcc ttgtgcaggc ctggaagaaa
    24901 gcaagtctcg cggaatggaa tgggtcctca gcatgcagag ctcgtgcggc ggatgggcgg
    24961 cctttgaccg ggacaacacc atgacgatcc tcaatcggat tccttttgcc gaccaggaag
    25021 ccatggtcga ctatcccacg gcggacgtta ccggaagagt gctcgaggcc atgggatact
    25081 acgggtacca acggtctcac ccccgggctc agaaggccat ccgattcctc aaggacctgc
    25141 aggagcctga cggctgttgg tggggtcgat ggggagtgaa ttacatctac ggtacctggt
    25201 cggtgttgcg gggtttgatc gggatagggg aagatcccaa gttgccttat atccaggcgg
    25261 ccttgcagtg ggtgaaagac catcagaacc ccgacggagg ctggggagag acctgcgaat
    25321 cctactgcag gcccgagctt cgggggcagg ggcccagcac gccgtcacag acggcctggg
    25381 cggtgatggc gctgcttgca gggggggagc atggaagtcc cgaggtacgc aaaggggtgc
    25441 agtacttgct ccgaaaccag cagccggacg gcacgtggga ggaaatccac tttaccggca
    25501 ccggctttcc caaccacttc tacattcggt atcacaacta ccgcaattgc tttcctctaa
    25561 tggctctggg ccagtacctt caaacattga gggaccggcc gtcctcctga tggggccgcc
    25621 gtttctcagg ggcccttctt ttttcccttc atcttggcga ctttggtctt ttcctcttcg
    25681 gccatggcga aggtgtgttc tggaccgggg aaacgcccct gttccacttc ttcccggtag
    25741 agggtgaggg cttctcggat gggtttgccc agccgggcat actgcttgac gaagcgaggc
    25801 aggaagcggt catagagacc cagcacatcg tggatgacca gcacctggcc gtcgcagtcc
    25861 ggtccggcac cgatcccgat ggtgggcacg gggacggttt cggtgatccg agcggccacc
    25921 ggcgacggga tggcttccag gacgatgctg aaacacccgg cttccgcgag ggcttgggcg
    25981 tcctcgatga gagccttggc ggcgccggcg gtctttccct gcaccttgaa tcctcccagc
    26041 tgagcactgc tctggggggt gagcccgatg tgaccctgga ccggaatccc cgccgccaca
    26101 atggcttcca cttggggaac cacccgtttc cccccctcca gctttacggc ttgggctctt
    26161 ccctctttga ggaagcgccc cgcattcaag accgcctgct cgaccccgca ttggtaggat
    26221 agaaagggca tgtccccgat gatcagagct cgtttggccc cgcgggcaac ggcgcgcgtg
    26281 tggtgcagca tttcttccat gccgacggaa agggtgtcct cctggcccaa aaccaccatg
    26341 gccaaggagt cccccaccag gatcatgtcc attccgctct cgtccaccca aaccgccgaa
    26401 gggtaatcat aggcggtcag catggtgagt tttcgttttc ctttggctgc cagaacatcg
    26461 ggggcggtga tacgcttgct cacgggtctt tctcctttca tcattccgtc tgaggagggg
    26521 ggggttgaag gtatccggct tccagggtct tgacaagctg ggtcagagtt tcgttcacgg
    26581 gcaaggtaag acccaaccgt cgtccaagtt ccacgatggc cccgttgatt ccatcgattt
    26641 ccgttctctt cttgcgatcc acatcctgtc ccatggagga acgattgacg gcggtggcgc
    26701 gggcaacgtt caggacccgc tccaccatat ccttctgaat cacaaaccct ttggcggctg
    26761 caacctccat ggcctcacga acggctctcg tgcaaaggtc cctggccgtg ggcacgtccg
    26821 ccacaagacc attgcggata ttggcgaggg cggtgatggc gttgatccct acgtttacgt
    26881 ggagcttctc ccaaatgagc tgttccatcc ggtcggaggc ttccgtctgg agacgggcct
    26941 ttcgaaacag atccaccacg ccgtaaaccc gttccgaagg ggggccctgg ggttccccga
    27001 cataagtcaa accgttgccc ccgtggcgca ctcttcccgg ctccaccagg gtggaccctt
    27061 gggcggtgct tcccaccagg actccctcaa tccctacaag gctcgcgatg cgctcccagt
    27121 tgccgatccc gttctggagg gtcaggaaga aggtcttaga atggcaacag gaggcaacgg
    27181 tgggaacggc ctgaagggtg tcgtagagtt tcaccagcac gatgaccaaa tccgccggct
    27241 ccgtgatggc ggcgggatcg taatggctca ccaattcgaa ccgctgttgt gtcccgtcca
    27301 gttcgtcgac gaggagccct ttccttctaa tggcctcgat gtgctgccga ttcgtgctca
    27361 gcagcgttac ccgggcgtcg gtcctggata agcgggcccc caagagactg cccaaggccc
    27421 cggcccccaa aatcagaaca tgcatgactc cacctctcgg aatctttcat tctcccgcgg
    27481 cggcaaagac tcgagaaagc cgccggtacg tgtgcaaggg atgcagccac ccgttgtttt
    27541 tcggctggac ataaaagcac cgggcacttg cgaacactat aggttcaaaa aagaaagata
    27601 aaaagcaaaa aaatcaccag aaccgtgacc aaaacggaca ccatcaagaa aacgtccggt
    27661 aggggcgaaa aacccgtttc accccggttg atgtgagaac ggatgtaaaa aaatcgccaa
    27721 cccgccagta tgatgatcat cgctcccagg ccgaaggtca ccaggcccag gtaatgcgtc
    27781 tgggaaacgc gtttcacgtt gctggagaag gcctgaatct cccggatgag aatatcgaac
    27841 cgttcgatga cgaacccaaa gacgatgaga gaaattcccg tgcgacacca ggcaagaaac
    27901 gtcctctcat tggccatgtg gtttctttgc caggcaaaga acaccctttc gtcggagaaa
    27961 tcgaagaggt cctggggctt caggggctct ttcgtcacag gtgcggtctt ggtctcttcc
    28021 atagtcgcct cccccggcaa gggaagttcc cacctgggaa actcccggga ccggggtccc
    28081 ggtctcgagt ttgggccggg tcttttcccc gtgcaccggg agtttgggaa ccgttttgga
    28141 gggatttgcc gagatttcag cgcagtttta gcgtggccag ggaaatgagg gccaggatcc
    28201 cggcaacgat ccaaagacgg atcaccacct tggtttcggc attgcccttg tactgcaggt
    28261 tgtggtgcaa aggtgcccgg aaaaagaccc ggcgacccaa ccagcgaacc ccgaccttgt
    28321 cttgaaactg gcttgtgagg gcctcggcga gaaacaagcc ccccaacaga gggaaaagca
    28381 tttcctgctt gaggagcacg gagatcacgg ccatgattcc tcctatggcc agggaccccg
    28441 tatcccccat aaagatctgc gccgggtagg cattgtacca caggaaaccc aatccgcccc
    28501 ctacgaaagc cgctccgaaa atggtcaatt cactcacgcc ccgcagatag gggtaataga
    28561 gatacgcgga gtagatccga tttccttcca cgtacgcaaa gatgcccagg acgcccacca
    28621 cgaacaggga cggagtgatg gccagcccgt ccaacccgtc ggtgatgttc acggcattgg
    28681 agacaaagat gacaaagaag aagatgaacg ccccgtagaa atagggtccc gggtcccaga
    28741 cggcgtgctt gtaaaaaggg atgtagagct tggtggccag gggagcgccc agaggggcgt
    28801 aggggcccac gcacacccag gcaaaaagga gggcgaacaa gccctggaga aagagtttcg
    28861 ttcgttcgga cagtccccgg tctccgctct tccgcttcac tttggacagg tcgtccatga
    28921 atcccaggcc cccgaaccac aacatcccga ccaccgagca caggagaaag ggattgtgcc
    28981 agttccccca caggagcatg gagctcaata cggagccgat gatgagaact ccccccatgg
    29041 tgggggtgcc gctcttgtcg aatgccgagt gaacccccgt atcccgtacc tggtcgaaga
    29101 ggctgcggtg atgcatgaac agaatgaaat ggcgactgac caggaagata aagatcacgg
    29161 acgtcaaggc cgccatgatg gcgcgaaagg tgatgtagtt caccagcctg cagaatgaga
    29221 agacgtccga gaggggaatc aggatgttgc agatttcata aatcataccg gtggattcct
    29281 cccatcacga ttttcgttca aacccccgca tttcatgacg atggaccccg ggcatcaggg
    29341 ctcggaattc agtaggtccc gaacggactt gtaggtctgc ttgatggaga aatgagggcg
    29401 aaagtccgta gccgtcagaa tgaagcccag gagctcggag ctcagatgcg gggagggcgt
    29461 gatcgttcct cgttccaaca ggcgttttac ccctttgagg gccgcatccc gtacctgcca
    29521 gtagtggtgc tctcgaagtc tgagcaggat ctccagggct cgggagttgg tccctatttc
    29581 gcccaatgcc tcggccgcct gcaccaccac ctcgaaacac ggttcattca gccttttcac
    29641 aagggcctga aaccattctt cctttccgga tccgtaggag ccgaagcggg ccagcgcggc
    29701 gcaggcttgg gaccgaacct cgtaatacgg gtcttccatg gctgtgagaa gggcctgttc
    29761 cacttccgca tcgaagtggc ccatgacttg aatcgcctgg ataatattgc ggcgaatgaa
    29821 gccgacctgc tcaaaatctc cccccaggat ccgtttcacc cagtgtaccg gcgttcgatc
    29881 cgtgagcata tggagaaggg tggggatctt ctcccggtgc agggtgaggc ccgtcagctt
    29941 tacgcccacg ttccgatcct gccaggcttc atgactcagg agcccggaag cccgatgctg
    30001 gtagtagtgc aagtcgtttt catcccctat cacggagagg gggtgatatt ggtcgggggc
    30061 cctccgatat gcggcgctca aagtctgcag cagctgctgg ttgctgagaa ggggtcgaaa
    30121 gggcaccttg acgaggtcga gttccgagga tgcaacggct tggttctttc cgtagatctc
    30181 gttcaaaata cggtcgtttg cctgctgcct caaaaagctc cggctgtttc gagccatttg
    30241 ttccagggtg gacgggtcct tgagaagctg gaggatacat tccgccagca cgcgtccttc
    30301 cagtttctcg aggattcttc ccgcctccac cacggtgtct tcaaatagga tcacggctgc
    30361 cccgcaatgt ttcatggtgc gtgcgttcat cacctggtga tcgccgggca gattggcctt
    30421 ggggatgaga accgcaggct ttcccacaca ggatatctcg ttcaggcttc cggctccgct
    30481 tcggcacaca atcaagtcgc agacggagta gatatcggca atgttatgga aatagtcctg
    30541 ccggtaatag cattgggcaa gggaattctt ctgttcgggc gtgagggtct tttcgattcg
    30601 atcgagagtg tccgcggcgg catcgtaatc cgccgatctt gccaggccgg ttccgtgaat
    30661 gatgaagatc tcccggccat ggggcaggag gtggggcaag gagtccacaa cggcccggtt
    30721 gatggtgcgg gctccctggg accccccgaa ggcgaaaacc acccgtttgc catcgggaat
    30781 ccgaaacggc aggctctcca gtgcttcctg acggggcttc acggcaatgg atttgcggat
    30841 ggggtaaccg gtgacgaccc cgtttctcgg gaaaaaggaa agggtttgag gaaaggtgag
    30901 gagcacgcgg tccacgtatc tccctaaaag accgttcagc tgacccggga tgctgttttg
    30961 ctcgtgaaga aagaccttgg ccggggaaac gcgaagggtt cgaaggatca aggcggcaag
    31021 gatgacggga gcgctcacgt atcctcccgt agcgatcacc cagcgagggg cgaatattag
    31081 gaggtacgcc acagactgga cgactcccag tcccagcttg aacagaaaac gcacacaccg
    31141 gaaagaaggc ttgagaccgg gaaacccctc ggaaaaaacg aaccacaacg ggtatcccgc
    31201 tcgacgaaca atcacgctct ccgctttgcc tctcaccccc acatacagaa acttggcgca
    31261 ggggtcccgt ttcttgatgt cttcggcgat ggccaaggcg ggattcacgt gtcccccggt
    31321 gcctcccccc gtcagaagaa ctcgtacttc ccggcgcgca agggtcttcc agcgacgggc
    31381 gcaaagaaac accaccgccg aagccgtgag caaaaccaac aagatcatca gggaagggtc
    31441 cattctctca tatccggggt gccggcacgg gtcccggcca taatgccgta cggacccggt
    31501 cggatcgttg agttaccagg aagtccgacg actcaaggcc cgggcgatta gttccgcggg
    31561 aagacagccc tcacgcacca gccgaggtac cggacggctt acatccagga cggtggacgg
    31621 caagttcccc gggagcgtcc ctgcatccag cattccgtcc acttcccgga gcaggtccgg
    31681 agggatctct gccgggtccg caatggggga acttccggca atgttggcgc tcgttgcggt
    31741 cacaagtcca cctactgcgg tcgcaagcgc tccggctacg gggtgggagg aaacccgcac
    31801 ggcgatcttt cccggcccgg tcagcaacat tgaagggaac gggacggcgg tgggaaggat
    31861 caaggtcagg ggccccggcc aaaaggcgcg gacgagcagg tccgtggaag gactccagtc
    31921 ggcggcgacg ttgcgcaccg cagcgatatc ggaggcgatg agcggcatcg ctttggcgag
    31981 ggatcttctc ttgatccggt aggcccggag gaccgcatcg gccgagaagg gattggctcc
    32041 caggccgtaa aaggtttccg tgggatagat caccaccccg tcccttgcca gccgtgccgc
    32101 agcctcggcg atgaacggat ccggcggccc ggacccgttg attctccaaa tccggtcccg
    32161 atacttcatg tccgcctcac gaggtctttc gctccgagag gtgttcctgc aatttattgt
    32221 tcttgtgcag gagcacgtct cgtttcatat cctgcttatg attttccagc ttccgcgcca
    32281 ggttcgggtc caggagcgcc aggatctgga cggcaaaaag cgcggcgttc tgtgctcctc
    32341 ccttgccgat ggacattgtg gctacgggga ctcccggggg catctgggcc gtggaaagca
    32401 atgcatccaa accctggaga ggggacgagt cgatggggat cccgatcacg ggaagggtgc
    32461 tttgagccgc cagggcccct gccagatggg ccgcccaacc cgccccggca atgaggacct
    32521 tcaggccttt ggcagccgat tcccgggcgt agctcgccgt ctcgtcggga cagcggtgag
    32581 ccgaaaggat ttgcacctcg taggggagac cgaagtcctc gagcacctga atcgcccccg
    32641 acatgacggg aagatcggaa tcgcttccca ttacgatacc taccagcgga tgtggccttt
    32701 ctgtcatggt tgtctacccg attgaatccg gttcgttggg attgcagcct cattccaggc
    32761 cgatgtcgct ccgaaaatag agaccctcga aggagatctt tttcatttcc tcgagagcaa
    32821 cggcccgggc ctcggcaagg gtctcgcccc ggctcaccag gcatagaacc cgtcctcccg
    32881 tggtgatcca ggtcccgttg gaatccgttt ccgtgcccga atggaacacg aggacccggg
    32941 gatcgatttg gtcgagtccc gtgatgggga ggccgatctt gtagcgatcg ggataccctt
    33001 tgtaccagcc cttcttcccc ttggtgggac cgctgacggc aatgaggcag aggcgatagc
    33061 cggggtccca cacgagccgg gtttgcgcca aacggcccgt gagtatggct tcgcagatgt
    33121 ccaccaggtc cgtctccaat ctcgggagga tcacctgggc ctcggggtct cccagacgaa
    33181 tgttgatctc gagcacgtac gggacgatgc cggagctttc gttcaccagc atgagtccca
    33241 ggtaaaggat gccccggtat acaattccgt gcttttcacg gattccgtga atcagcggtt
    33301 ccgccacccg ggccatgatg gtccgaacga gggcgtcgtc caaccaagga tggggcgaat
    33361 aggctcccat cccccccgtg ttctttcccc ggtcgccttc ccgggcccgt ttatagtcct
    33421 gggcggccac catgggaacc acggagtacc cgtcggtaaa gcaaaagaaa gacaattccc
    33481 ggccgtagag gcgcctttcc acgtccacgc gggcccccgc gtccccgaag atgcgctctt
    33541 ccatcacggc gcggacggcc tcccgggcat cctcgacgga atcacagacc agggatccct
    33601 tcccggccgc cagtccgtcg gccttcacca ccacgggata gccgacctgg cccacatagt
    33661 ccttcgcctt ttgaggatcg gaaaagacct cgaagtcggg aacggggatt cccaggtctc
    33721 tcagcaagat cttggtgtcg cacttgctgg attccaggca gctcgttcgt ttgtcgggcc
    33781 caacgatagc cagtcccttg ctctcgaaca ggtccacaat gcctgcggaa aggggtttct
    33841 cgggccccac aaaggtcagg tcgatgcgct ccttttccgc aaaggccgcc atgtcttcga
    33901 tgcttcgagc gtccacgcag gtggccgtcc gagcaatgcc ggcgttgccg tgggccacgt
    33961 agacgtgctt aacccgggga ctctgggcga acttccaggc aatggcgtga tctcttccgc
    34021 cgcttccgat aaccagaatt ttcatgaagg tatcttccca gctctcgatt tgaaccccaa
    34081 tgccaaggcc ccggccacaa gaaacgcagt ttcccctccc ggtcctctcc cgcccggcag
    34141 gggcaggggc gggaactcgt tcccggatca atctcgggcc gaaccgttca ccacggaccg
    34201 aagatactcg cagatgtttc cgtcgtaggc gtgggtcagg caaaatgcct tgcaggcaag
    34261 gctcatgctc gtcttgcggg acaacgaccc cccgtgtccg ttcatttcct caaggatgag
    34321 ggggtagtcc tcgggatcgg tgaccacggc cacatggcga tggttcttgg ctgaggctcg
    34381 tatgagggtc ggacccccga tatcgatgtt ctcgacggct tcctccaggg tgcacccggg
    34441 gcgggccacg gttttggcaa aagcatacag gttcaccacc accatatcga tgggttcgat
    34501 cccgtgcttc ctcatggtct cgaggtgctc cccgttgtct cgaatcccca ggatcccccc
    34561 gtgtattttg ggatgcaggg ttttgaccct tccgtccagc atttccggga atcccgtgta
    34621 gtccgagact tcggtaatgg caatgccgtt gtcccgaagc aggcgagcgg tgcccccggt
    34681 ggacaacagg tgcacccccc atcgctccag gccccgagcg aagtcaacca accccgtctt
    34741 gtccgtcaca ctgatgagtg cgcgacgaat tggaaacata cttcctcctc caaagggttc
    34801 catgatgcaa acatgcgctg ctcgatgatc cctcccggat ccgggcattc ccggaccgat
    34861 cgaccggcag ggtgaaggtc ctccggcagg gagcctccga gaagggcgta ccgtcttccg
    34921 gcattcatac cgcggggacc cgaccggtcc gatgaggagt ccgggcgagg cggaactatt
    34981 gaagccggat tccggccaat ctccggaaaa gaactcacat aaaccaggct catggcgggt
    35041 gtccagactt atttggacat ccccgaaact atccgggacc gactccctcg gaggacaagg
    35101 gtgaaagggg gtcaaaggtc gtgcggaaaa ctcccccccc acccttccca tccccgggga
    35161 ggatcggttc cgaatctcgg gatgatcccc tattcacgat tccgggaaaa gagggcgtcg
    35221 aagatcgaat cggccgcttt cacgataagg gcatcctcgg ggagcgtcag ggtcggtttg
    35281 ccctcaaaat catactgggc cacgagcggg tccttgggca gtacgtggat gtgatcttcc
    35341 ccaaaggttt gaatcacctc ttccggccac gacggaggag aggagtccat ttggttgacc
    35401 aaaagcctca cctcggcgac cttcatgggg agggtgcgca ccagttcggc aatacggcag
    35461 gccgcggcaa ggcctcgacg actggggtcc gacaccagca ggagaaagtc gatgtccttc
    35521 tgagtgagac gactgaaatg ctccatgccc gcctcattgt ccaccaccag gtaacggtag
    35581 ttcttgatca gccgatccat gaggctgctc agtaaattgt ttgcggcaca ataacagccg
    35641 gggccctcgg gccgccccat ggccaccaga tcgaagccct ccgcctccac cagggactgt
    35701 tccatcttca tctgcatgaa cacatctttg gtcatgcctg tcggaacgga ggtcttcatc
    35761 atctcccggg catcggacag ggtctcgtcc aggcaaacgc ccaggacatc gttcaggttt
    35821 gcattggagt cggcatcaat ggccagcacc ggcttcatcc cgtgcttgat gaaatatcgg
    35881 atcagcaagc ccgctatggt ggtcttgccg gtaccgcctt ttcctcccaa agccactgta
    35941 aatcccacga acgatactcc ttgattccaa caaggatgca aacggcaggt cccggatcct
    36001 cccctcgggc aatcccggtc aagcgtctcc cttggaaccg taggtccaca atgccgcgca
    36061 aaagccccaa acggcattgt agaagaatac aaaaaccggc gtcagcttgc ccagagtcaa
    36121 ccccaagacg gctttgccct ggagcaccgg gaatacgtaa agcagctgga agagagtagg
    36181 caggaggctg aaaaggacgc ctcgggcaaa cacgccgacc caaaatccgg acctccaggc
    36241 gggagccaga aaaaccagtc cccacaatcc cccccataca agcctgggat acagatactg
    36301 ggcactccac gccggggcga tggccacgcc gaattgctga gggatgccct tcctccccag
    36361 gtaccatgcc agcaaggaat tcacgaaggc cccaaagacc cctcccgcaa acacgatact
    36421 gatccgacgc attgatccgg acataagttt atctggactt tcacccggtc ggaacgattc
    36481 ccgacccggg ccggccgttc gtctcgaacg ccgagtgaga agtctgtgca cgcggcattg
    36541 ccgccttcgg ctcggcaccc tgcccaattg aaaattgata acataggaca aaagatttat
    36601 aaagttctat gcttctccat tggtgggaga aagggaatgg agaagtaaat ctctcgggtg
    36661 gaaggacagg tctcgggctc ggagccaagg agcgtcgtcc gaaggccgaa caggagcggg
    36721 tcgcgtcctt atggatctta cggtaagaca ggctgcgaat cgattgaagg tgagcgagag
    36781 caaggtgaga gactacatcg cctcggggac gttgcccacc aggaagaagg ggcgagggac
    36841 cgtcattccc gaggatgccg ttgagggtct cctcaaacag tcgcctccag gggacgcaat
    36901 gcctccgggc cctgacgaaa cgctccggga catgttggcg tctctcctcc aaaaggtggc
    36961 ggaactggaa cgacaggtgg acgaaaaatg gagcctttgg agggagaatc ggcgactctt
    37021 ggaagaactg aaccagagtc ggcggcaact ggctgagaag acctccgagg tcgagagact
    37081 tcagcgggac cttctctacc agaaacgatt gcttgaaaag gaactgcagg attgccgaac
    37141 cgcgatggag gagaagggcg cgtggatcga gaaacaatgc cgggagagga tagccgggga
    37201 aagggagttg cttctcgatc gtttggccct tcaggaaaaa cacctggaag aaaagctcga
    37261 gtgggagaag gaaaagtacg gacggaaact cctggaaatc cagcaacggg agggcttctg
    37321 gtccaagttg gcccgaatga tgacctggag ctgacggcgg gttgaaccca acggccgaaa
    37381 cgggagggcc cggtaagggt gccgggggtt ttcggaggcg gcctcatcgg atggagaatg
    37441 attgaattca tgaaaaatcg actcatagag ctgatcctgg attacgcgtt ccagtattcg
    37501 gaaaaacccg tctttaggct ggttcacggc gggatgagcc ggttttactt caattgtaag
    37561 caagtgaccc tggaccccga gggccaatac ctcatcggcc acttggtact ggagaagatc
    37621 cgggacctgc aagtccgggc cattggggga ctgaccctgg gggcggatcc cattgccaat
    37681 gccgtatcct atacttcctg gctccggaag gaccccattc aagcctttgt ggttcgaaag
    37741 aagaccaaag atcatggaac ggggagcctc attgaaggaa aagtgcgccc gggggatcgc
    37801 gtggtcgtgg tggatgacgt gatcaccacg ggggggtcca cgatacaggc cgtggaagca
    37861 tgccgatctt acggactcga ggtcgtaaaa gtcgtggtgt tggtggaccg tcaagagatg
    37921 gggggcagag agagcatcct gcaaacggtt ccggctcttg atgctttggt aactcgagag
    37981 gaaatcatgc aagtctacgg ggagggcgtg atccatgact gatgagccca agggaactca
    38041 ggatgcctgc atcgaaccct gggaagggga gcgggcggag gcggccaatc gcatctgctg
    38101 cagggcttgt gatgcgaccg gggtcaaggt gtcttcgtgg gagcagttcg atgcctggaa
    38161 acaatacgtg gatgggaaga tcgaggaagc cgaactgacc gaacaagcca gacgcgagct
    38221 ggatgagttc tccacggttt ttggcaagta cctggtgatc gagcaggagc aaccgaagca
    38281 aaccgacaag gaagccgaga agaagaagcg ggcccgacgg gccaacgcca tctaccggga
    38341 agtgtgccgg gaggcgggac tgagcgtgta cttctttcat gatttcctca gctggagcga
    38401 cttcgtggcc ggcaatatcg gcgaatcgga gttctacgac agggcacggc gagaggtcat
    38461 ggaaatccgg ggccgtcccc actgacgttg tccccgagaa tctccagagg gagcaatgga
    38521 atgcgcgagc gcgagctgac tcatatcgac tccagcggcc gcctgcgaat ggtggacgtg
    38581 accgaaaaga acattacgga acgcgaggcc agggccgagg cgaccctccg attgtcacgg
    38641 gagacactcc gacagatcct ggatcggaaa gtgcccaagg gcaacgtgtt ggaggctgcc
    38701 agattggcag gaatccttgc ggccaagaag acttgggagc tcattccctt gtgtcatccc
    38761 atccacctga gcggcatccg ggtggatttc ctggtcgagg aggagcggag cgaaatcaag
    38821 gtcctgtcta cggtgaaggc ccttgatcgc acgggagtgg agatggaggc cctggttgcg
    38881 gcgacccatg cctctctgac catctatgac atgtgcaagg ccattgaccg agagatgtcc
    38941 attcacgagg taaggctgac ctacaaatcc ggggggcgaa gcgggaccta ccagcgggcc
    39001 tagaagctac atttcccctt tactcttttc cgggattatg ctagccagga aacctttaat
    39061 ctatctctgg gggtggaact atggatcggg agatgcaggc tgtctttaag gaactgctgc
    39121 tcaagcgact ggacgaattg taccaggagg cggaaaaaac ggttgccggg atgaccgata
    39181 cggaagaaac gttccctgac cccacggacc gggcaaccct cgagtccgac cgtaacttta
    39241 tgcttcgaat ccgggaccgc gaacgcaagc tcatcatgaa gatccgggaa gccctccaac
    39301 gaatcgagga cggcaacttc gggatctgtg aaatgtgtgg ggacgatatc ggcatcgacc
    39361 ggttgaaagc ccggcccgta accactcagt gtattgagtg caaaagaaag caggaggcgg
    39421 cggaaagggc caggggcggt tgacgctcaa ctctcgaggg cgccgtctgc agactccccc
    39481 gggacacttc ccaggacttg ggtcacatcc ccgacccaga tgatcttttc cacgtggtcc
    39541 tccgtcaaca gccggagggc tccttccggg gtaaagccca acacttttcc ttccagctct
    39601 tcttcccccc tcagaattcg gatggttttg cccaaaaccg cagaataccg ctccagttcg
    39661 gtcagaagag tctcaaatcc ttcccggatg aagcgggagt actcccgctc gaattcatgc
    39721 aggtactcca gcaaaagctc ctgtctccgg atggatcggc ccagctcaac ggcaatggag
    39781 gaagcggggt atcggaaagg ccccccgagc tccgctgccg tgtagttggc attgataccc
    39841 accccgatca cctggaaccg ggtaaagtcc aagtccgact ggatttccgt aagaatgccc
    39901 gccacctttc gattccggat gagaatgtcg ttgggccatt tgatggcgga cggcaggcgg
    39961 tagcgggttc gaatcgcctt tgccagggca agggcggcca ccaaggttgt ttgggaagcg
    40021 atccggatgg gcaagggggt tcgaaggagc atggaaaaat agatcccctt ttgggatgca
    40081 gaaagccatt cgcgcttcaa ccggcccctt ccatgggtct gttcttcggc aacgaccacc
    40141 gttccgtggg gggcgtcccg ggctgcaagc aacaacgcgt ggtcgttggt cgaggctacc
    40201 cgggagaaat gatggtaaga cctgcccagc cactgggtct cgagattggg caggatttcc
    40261 tccgggatca gcaggtccgg gacgtccagc aaccggtatc cctccttggg atggctttca
    40321 atgtcatagc ccatctgccg aagattctgt atgtgcttcc agatggccgt tctcgtcaga
    40381 ccgaggccgg tagccacgaa gcttcccgaa agatacccac ccttttgatc ttttagcagt
    40441 tgcaggatct gccaatgagt cgggtcttgc gcaaccatca ttcccaattc cttgaattct
    40501 caaaagagaa gcccttccaa tgccgggaag gatcaacgga ctccgtaatg cacagggagg
    40561 cggccttccc ggccttgccg gagggaccgc ggatctctcc caagaaggtt tgccgggtcc
    40621 gtccaatcat caggaggcca aaaccatggc ccccaaagca taaagaagga tttccgtcat
    40681 ctcgttcacg gctcccagaa catctcccgt aacgcctccg aaccactttc gacaaccccg
    40741 tttcatgacc cgggaaaaga cgagcaccgt cgccgcgcac aggagcgtaa agaaaggatt
    40801 cacgactgcg ctcaacaaga ggcatattcc cagggcgatt cgcagttccc tggcgctcat
    40861 gtgctcgaga aagggcttcc ccaggcctcc ctcctttcgt gcgtaaggcg tcttgtaggc
    40921 ggtgagcacc attgccaggc gactgaaggc ggggactatg agcagaggcg agcctagccg
    40981 atgggtcagg agggcgtgaa gggccgcaat cttcaaggcc acggccaggg agagcgcgac
    41041 cgcgccgaac gctcccgtgc ggctgtcctt catgatctcc agacgtcgat ccgggttgta
    41101 tccccctccg aggccgtccc aaaaatctgc aagaccgtcc atatgaaatg cccgggtgac
    41161 gaggaccgaa tacagggtca ggatgcaggc caaaaggaag gggggggtgg tccgggccag
    41221 cagcagcgcc agtccccagg aaccccctcc caacaggaag ccgaccaggg ggaagaatga
    41281 aaagctcctg gccagggaga cgggtgtcgg ttcccttccg gtcatggacg gaacgtgcaa
    41341 gagggtcagg aaggacaggg ccgttacgaa atcgcaccac atcaggtcac ccgagaattc
    41401 cggtcgccgg ggcgtggata ggaccggttc ccggcttgga gaaggggacg atcgccgccg
    41461 aaatcgcggc atggcaggga aatccggaac ggtttggccg caagagggga ctcatgcctt
    41521 ttccgaatgc cccgcaattc ccggcggggt aagattcggc cctttcaaaa cccacgccag
    41581 gccggccacc acgtaggtta ccgaggcgca tgccccggca aattcccggt ttgccgttcc
    41641 ggccaagtcc cgaaatcttc tggccatggg attgtcgggc acgatgcccg ctcccacctc
    41701 gttggagacc aggatcagcg gataatcgac ctgaccgatg aagcctcgaa gctccgtgat
    41761 ttgggactcg acgaccccgg gcgaaaatcg cagcagaaga ttggacaacc agagagtgag
    41821 gcaatccact agaacgggaa gattccgtcc ttggaggttg cccagggttt ccgtgagccg
    41881 gatgggacat tccaaggttt cccagaaaga agacctgcgt tcccggtgga ttcgaatacg
    41941 ctccttcatt tcctcgtcca gcgcctgggc cgttgccaca tagatgtagg gaggaggaaa
    42001 tgcggtgatg agctcttcgg cccacttgct cttgccgctc ttggctccgc cgagaatcag
    42061 gtgaggggtc gaaggaaggt ccggggcact catgaggact cgatatcctg ggggaaaaag
    42121 ccccgcagat gttccaggac cagcttgtcc accaggtgcg gcaggggttc ccgtacggtg
    42181 cccacgatga cgatgggatc ctgggtgatg ggcaaaagga tcttggtcgc cttggctgct
    42241 ccgactgctc ggaccatgcc cggagtgacc tcccccatca tggaattggc aagaagaatg
    42301 gagatggggc ctatgatgac ctccacctcg ttgacgcaat ggcagatggc gttctccccc
    42361 gtagcccccc ggttagcccg ggccttcatc atttgagcgg ttgcgatggc attggtgccc
    42421 agggcccaga cttccgttcg ctccccgtac acgtccttga tctttcggat gatggtcgtt
    42481 ccaatccctc caccttgtcc gtcgattaca gcgatgcgga tcaaaacgtc ctccctttgc
    42541 ccttcggcaa ccctccggcg ggggcagtga acatgaaacc ggagagcatt atgggggcga
    42601 aatcacgggt cccgttcttc ctcctccccc ggtgggacga ggagactcca tgctcgaaac
    42661 agctcatgat atcggaggat gaactccttt ccatcttgag tctttacctt gaagtagcgg
    42721 agcggcacac gcctcgcgtc caggtagccg gaataccatc ggtccacgat ttcccggatc
    42781 cggtaccggt tgtttcgcca ctggaaggag atcggttctt gcgcttcccg gtattcgtcc
    42841 cgggtaagga cttggacccg ctcatactcc atgggccatc cactggttgc agaacggcgt
    42901 gcgcacggct cgtgcctgca attccacggg ggataggggt tttccatacg gcctcgaacc
    42961 tataaaatta tatgggtttt ctgtgacaat tgaaacgact ttttgatacc ctgcaaggga
    43021 cctcggcacg gccacggacg tggactcgtg gacaccaagt caaggtcctt gctcgatatc
    43081 ggtcaggtcc catgcaaggg agcatgaatc cggagatccg gaggaattga atcatggcac
    43141 gcaaagccct attggtgatc gatgttctga atgattttct ggaccctaaa ggtgccctct
    43201 attgcgggga cgactccaga aaaatcattc ccccaatccg tgttctgctg gaccgcttcg
    43261 cccgggacaa acacctgatt gtgttcctcc gggatgccca ttcagtggac gacaaggaat
    43321 tcgagctgtt ttccccgcat gccgtgaagg gcagctgggg aagtcaagtc attccggagt
    43381 tggcgccccc cgaaggcagt ctggtggtcg acaagacccg tttcagcggg ttttttggaa
    43441 atgacctgga caagattctt tcgtccgcgg gacccgagga agtctgggtg gcgggagtgg
    43501 tgacctccat ttgcgtcatg gatacggcgg gagaccttcg aaaccggaac tattcggtgg
    43561 tggttcccat ggatgcagtg gcggatttcg acccgaagtt ccacgaattt gccctgaccc
    43621 gcatgcagcg cgtctatggc gcgagactgg tgaagacgac ggaagactga tccacggttt
    43681 ccaaccgccg gccatcgaga gggtcgccct atgagtgcaa gtcgacggta tgctcccgag
    43741 ttggtcacgg acttgtatga actgaccatg gcggcaagct atttgcggga agggatgatc
    43801 cgggaggcga ccttcagcct gttcatccgc gattatcctc cggaccgcgc ctactttgtc
    43861 tgcgccggga tcgaagacct ggtggaaacc gtcaccgatc tgaatttcag ccactcctcc
    43921 ctggagtacc tctcctccct ggaaaagttc ccccgggagt ttctcgacta tttgcggcgg
    43981 tttcgatttt cgggaaccat ccgggcaatg ccggaaggga ggatcttttt tgcccaggag
    44041 cccatcctgg aagtgacggc tcccctcatc gaaggacaaa tcctggaaac cctcatcctg
    44101 aatacgatcc agctggaaac cctcattgcc ggcaaggcag cccgctgcgt ccatgccgcc
    44161 cggggaagag gccttattga tttctccttg cgcaggactc aggggattca ggcgggactg
    44221 aaagcagcga ggggctccta tattgcgggc tttctgggaa ccagcaatct catggcgggc
    44281 gagtggtaca aaatcccggt tttcggcacc atggcccact cctacgtcac gagcttccct
    44341 tcggagatgg atgcgttcgt tgcctatgcc cgggcctttc ccgacaatac ggtgcttctc
    44401 atcgacacct acgacaccct gagcggcgcc cggaaggccg tccaagtggc aaagcggatg
    44461 agggacgagg gcaaacagct cgtcggcgtt cgcctggaca gcggggacct ggcggaattg
    44521 agcaagcagg ttcgggccat cctcgtaggg gaaggcttcc ccgacgtgat gatcttcgcc
    44581 agcgggaacc tggatgagta caaggtggag gaactggtcc gggcgggggc cgaaatcgat
    44641 atttttgccg tggggacccg catgggggtg tcggcggatt ccccctacct tgatattgcc
    44701 tacaagctgg tggaatacga cggaaggccc gttttgaagt tgagcgccgg caagaagacc
    44761 tgggtgggca gaaagcaggt ctaccgctct cgggatgggg aggggaagat gaaagaagac
    44821 cggttggccc ttgccgggga aaaggttccc ggagcggaac cgctgctgga gacggtggtg
    44881 gaaggcggca gactcgtgca tcccctggaa actccggcga ccatccggga gcgattcagg
    44941 caagagtggg aaggacttcc cccgatctat cgggagttgc gtccccggca ccgttatccc
    45001 gtatccatcg gtgcggaact gggtgcgctg caggagaaaa cgatacgcga gaaaagtctc
    45061 caggagattg attgagacga agtccccggg gaagagtccg cttcagaggc ctttttccca
    45121 aagccacagg tatcgaggtt tgtgcagacc cgggacggta taaggatgct ttcctcgaaa
    45181 ccgcagggaa cccggggagg gggtcgattc gtgcagggat tcgggcggaa aggccagttc
    45241 ccccccccaa taggccagga ctccccccgg ccccagggag tcccgggcat agtcgccgaa
    45301 ccttcgcgga tccaagcgca cggagcgcag agcgatcagc tcgtatttcc ggggaatgcc
    45361 cgggacggca agtcgctccc actcttccca ccggccttgc acggcggcaa tggatttcat
    45421 gcccaaacgg gcgagcagga cccggagaaa gctcaccttc ttgcggttgg actccaggag
    45481 caccaccgtt cgatgggaag aaaagatctt caggggaact ccggggaatc ccggcccgga
    45541 tcccacatcc aggatgctcc cttcccgggg cagccattgg gcggggatga gtgaatccag
    45601 gatatgctcg ttcaggatct gttcaggttc ggtgattctc gtgaggttga cccggcgatt
    45661 ccattccagc atgagctcca catgctgaaa aaccctgtgg gcctgctctt ccgagaaggc
    45721 gatgccgtgt tcccggcact tccggagaag cagggacaca aaagggtcct ctttcatttc
    45781 attttccgat gcaaaactga gagaagattc ggtctagaag ctcgtcatcc ggttctcggc
    45841 cgaggatggc atccagctgt cggcgcgcgg agtcgagctc gaggcacgcc agttccgtgt
    45901 cttttcccga tgcgaggagc ttctgtgttt cccgcagtgc ccgaagcgcc ccctccaaac
    45961 attctttctg gcgcaggttg ggaatgaatt ccgaacggcg ggtgtccagg ggacgggaga
    46021 ggtaacggtc cgccagggtt tcccggagcc tttcgatgtc tcgggggttc aacaccgaaa
    46081 ggggcatgat caagggtgag ccgccgtagc gtttccggac gtcctccggg gagaggtgcg
    46141 gggacaaatc cgacttgttc aataggatca tgaatgggat gccggatagg gcctcgaaga
    46201 ccgcgtcgtc ctccggggtc aaggagctgc tccggtcgat cagccaaagg gcgatgtccg
    46261 cctcatctac ggagcgcagg gtcctttcaa tccccaggga ctcgatttcg tcgggctccc
    46321 ggcgtattcc cgcggtgtcc aggattctta cgagtattcc cgaaagcagg aacgtgtcct
    46381 cgatgacgtc tcgtgtcgtc cccggcaaag gggtcacgat ggcccgatct ttacccagca
    46441 ggacgttcaa cagggaggac ttcccaacgt tgggctttcc cacgagaacc agggtcagtc
    46501 cctcccgaag cacccgcgtg ctttcgtatt cttccaggta acgggtcaaa gggcgacaca
    46561 ggtcttgctc caagctttcg gcccaggctt cccagcctga agcttcccct tcctccagat
    46621 cgtccgaaaa gtcgatggat gcctcgagac gggcttggag ctcgaggagg ctttcccgcc
    46681 aggattggac ccttgtgcga cagccgccgc gcaaggatcg gtttgccatg agcagatggt
    46741 ggtgggattt ggaacggatc acttcgatca cggcttccgc ctgggaaaga tcgatgcgtc
    46801 cgtggagaaa agcccgccgc gtgaactccc ccggttcggc cagccgcacc ccggatttca
    46861 acaccagttg aagaatgtga tcgaggaccc agtagccgct gtggcagttg atctccacaa
    46921 cgtcctcccg ggtgtaggta tgaggggccg ccatgtagct cacgaggact tcgtcaacgg
    46981 cctggccgga ctcggggtca tggatccacc catgatggag gcggtgggat tgcagggtcc
    47041 caacgctctt gtggggccgg aaaaggcgtt ggagaacttg gaaggccccg gccccgctga
    47101 tcttgaggat cccgatgccc ccttcgccca cgggtgtggc gacggcacaa atggtatcat
    47161 ccggggcttc gggtcccatg ggctccgaga acctgctgca tcccgtccta ctcctgggcg
    47221 gcaacttcgg gggatttctt gccgcgcttg accgggtaga tgaccactcg ccgcagattg
    47281 ccttccccct tgctcttggt tcgaacctct tcatcctctt tgagcgcaag gtgaatgacc
    47341 cgacgatcct gggcgttcat ggggcccgtg gtcaaagggc ggcgggattt cttgactttt
    47401 tcactcagct gctgggcaag ttccgtcaag ctgacttctc tcttggatcg ataattctct
    47461 gtgtcgacga tgaccgagat gttttcaccc atctcctttt gcacgatctt gccgaccagg
    47521 tactgaagag cgttcagggt cttgccgcgt ttgccgatga gaatgcccga tccgttgctg
    47581 atgatgttca ggcagacgtg gtcttcgtgg gcgtccggtt cgaccaccgt gggcaaatcc
    47641 acgtatttca agacttccac caggacttct ttggctcttt ccgcaatcgc ctcgggggcc
    47701 gtcaccttgg aggtgacccg aatttttgcc ttcttggcac cgacaatgcc gaagattccg
    47761 gaagatcctt tggaaaggac ctcgatttcg agcttctcac gaggaagctt gagtctttca
    47821 catgccaggg caatggcatc ctcgacggat ttctcttcta tttccagggt tggcatgctc
    47881 tcctccataa tttctcggat cgagtcggtc attatgcgac ggaatcgagg agatggggcg
    47941 tcgatcatgc tttgtggttg atccagtatt gttggacgat cgacagaatg ttgttcacaa
    48001 gccaatataa cacaagaccc gagggaaagt tgacaaagaa aacggtgaat accaccggca
    48061 tgagtagcat cagcttgtcc tggcgagggt cgccggtggc gggagtcatc ttctgttgaa
    48121 cgaacatgga gattcccatg aggatcgtga gaacggggac gccgtccagg taggggatgg
    48181 gaatgccgat gtggaggcgg tccggcgcgg tcaagtcgtt gatccagagc atgaagggct
    48241 cgtgacggag atcgatggct gcgttgagca tccgatacag ggcgaagaag accgggattt
    48301 gcagaagcat gggaagacac ccgcccagcg gattgacctt gtaggtgcga taaaccgcca
    48361 tgagctcctg gtttagcttt tccttgtcgt tcttgtactt ctcccggatc tgggtgatct
    48421 tgggttggat cttcttcatg gtttgcatgg attcgaagct cttctgggtc aagggccaga
    48481 agatgacctt gatgacgatg gtgagcagga tgatggcaat cccgtagttg tgggtgtatt
    48541 ggaagaccca gttcatgcca aggagaaggg gcttggccag gaaagtgaac cagccgtaat
    48601 ccacggacgc ggaaagctgg tgttccgctt tgcccagttg ggtcagttcc ttgggaccca
    48661 ggtagatccg caaggggatc tttcttgcct gccccccttc gatctgaaac ggctccgtca
    48721 ggtagaccag ctgcaagagg ccctccttgg ggtccaaaac cctcgcaacc acctggtagc
    48781 cctgcttctc gacagggagc accgcctgaa tgaaatagtt gttttcatac ccgacccagt
    48841 cgatgggtcc tttgaggacc agctcctttt tctccagatc gctcagactg tgattggcga
    48901 tctttccttt ctcgtgggag gacagctgtg acgggttgta ggaactttcc gaggccaggg
    48961 gggaatacgg ctgaaagtag aagctgatcc ccatttggtc cgagagggtc ttttccgaaa
    49021 ggttttccag ctgtatctcc acatcgacga aataggcatc cgccgaaaag gtgtagctct
    49081 ttgtcactct tacctcgccc gggatttccg tttggagggc caggacctgg gaagggtttt
    49141 ccggggtcag ccggatttgc tccggcgccg gggatgaaaa aggtcgggtg gccaacgacc
    49201 aatcggagtg gttcatcagg tccaccgcca tgggaaggta tcccgtgggc tgggtggtga
    49261 cgatttgcat ggggggcgaa tccgggttca cctcccgtcg gtactttttc aggcttaccg
    49321 aatggattcg tgcgccgggg gccagcaact gggtcgaaaa caggggagcg tcgacggtcc
    49381 aactggggaa atgttgatcc agctttacct ggccccgggg aaggaccttg gggaccaccg
    49441 tgtcggggag cttcggtgtg ggtcgggggg cttgggacac gggacccgag ggggccgact
    49501 cctggggcgg cttggtatcc ggagctcgga agagcccgaa atagtagtcc caaaaaacca
    49561 ggacgaggag ggatagtaca aacgcaatta tggcccgttt ttccatctaa gaaagctcca
    49621 ctttgtgggg acatccgttg tagaaggggg atcgaccggg tcgtatcctc ccggatgaaa
    49681 ggggtgacag cgcgcaattc gcccaaggcc caggaggcaa cccttccacg gcccgtgcaa
    49741 ctgtacggcc tcataggtgt attgggaaca ggtcggaaca aagcggcagc agggacccat
    49801 ccatggggaa agaagggttt ggtagctgcg tatggcaaga agaaagaagg aacgaatcat
    49861 caagcggttc tcgtcttgct acttggtcaa aacttcggcc aattctcggg cgataccttc
    49921 gggaagcatg gtttccacgc ccggtccggc aacgaacacg atgtccttgc cgggcggggg
    49981 gatctcatgt ttgtgcaatc gaaaccattc cctgagccgc ctcttgatgc gattcctctg
    50041 aacggcgccc cagaatcgct tctgcaccac catgcccagg cggtgatggg ggaggttgtt
    50101 ggggcgccaa ttgatggtaa agtgggccgt ccgaacccgt ttccccgtgc gttttgcctg
    50161 ccggtagtct ttggaagacc tcattcgctc acagggacga tatggataat tgcccatccc
    50221 caggaacctc atcaaatggg gaaatccggt gcctcaacga ccccggtgat gagggctacg
    50281 ggctcaaccg cttgcgccct ctggcccgtc gccgctggat cacctttctt cctccttggg
    50341 tggccatgcg cgccaggaag ccgtgggtcc tctttcgctt gagattgctg ggctggaagg
    50401 tccttttcat tgcacttacc ctcatacttg gaataataca aagttggctc ccgggaaaag
    50461 gggaccaacc cgggggttcg gacgaccggc accggactct tgggagttca ggtgagcctt
    50521 tgccactatt aaatacaaac gaacctctat aatcgaatcc gcccatgact gtcaagaagc
    50581 aaaaggcatc gcggcacaca cgcttgttcc ctgcttctcg gctccaaaag ggtcccccga
    50641 agtccgaccc ctctggaaag ggggttgggt caggccatca cctctgcgca gagcgggcag
    50701 accccgaccc cccctttgga aagcgggtcg ggcggagggg agggggatcg tccggcgagg
    50761 ttgtttgcgg ccgctctgcc gggacaggat ggaaatcttg ccgacggaac ccgttcggga
    50821 accggccggg gggaagggtt ggatccgccg agccttcccg caggtgccgg gtgcgttcgc
    50881 ccggctgaaa acgctgcttc cgggatcgct tcctttgttc gtctaatcta ttgaagatcg
    50941 ggacctggca tgctatgaaa tgaagccttt gcggcaatca agggaaagga gacgggacgc
    51001 ggatgttctt agaccgaatt tttggctgga tctccaatga cttggctatc gacctgggta
    51061 cggcgaatac cctggtatat gttcgaggca agggaatcgt gttgtgtgaa ccctcggtcg
    51121 ttgcggttcg caaggatgaa cggggggcca atcgggtggt ggccgtcgga aaagatgcga
    51181 aactcatgct gggaaagacc cccggcaata ttgtcgccat tcgacccatg aaagacgggg
    51241 tcatcgccga ttttgaggtg gccgaagcca tgcttcgcta cttcattcag aaggtccaca
    51301 accggagaaa cctggtgcgt cccaggatca tcgtggccat cccgtcgggg gtgacccagg
    51361 tggagcgcag ggccgtgcgt gaatcggcgg aatccgccgg cgcacgggaa gtctacctca
    51421 tcgaggaacc tatggcggcg gccattggcg ccggtctccc catcacggaa cccgtatgca
    51481 atatggtagt ggatatcggg ggaggcacca ccgaagtcgc cgtgatctcg ctggcgggaa
    51541 tcgtgtacag ccggtcgatc cgggtggggg gggacaagat ggatttggcc gttctccaac
    51601 atatcaagcg caagtataat ctgctgatcg gcgagcgcac ggcggagcag gtcaaaaccg
    51661 ccatcggcaa tgcctacccc atcggtgaag cggaaacgat gatgatcaaa ggtcgggacc
    51721 tggtcagcgg gattcccaag accgtggaga tcaactcgga cgagatccga gaatccattc
    51781 aggaacaaat cgactccatc gtcgaaacca taaagattgc cctggaacag actcccccgg
    51841 agcttgcggc agacatcgtg gatcgcggga tcgtgctcac ggggggagga gcgctgctca
    51901 ggaatctgga ccacctgttg aggctggaga caggcttgcc cattacgctc acggaagacc
    51961 cgctgtcgac cgtggtgctc ggttcaggca aggcgctgga agagatcgac attttgaagg
    52021 acgttctcgc ctgagcccgt actgggacct ggacgaacga cggcccggcc ggtctgcgga
    52081 caaagcggct gacggggttt ggggtttatg atggcttggc tgcgccgcct tcgcgggacg
    52141 attctgatcg taattttcct gggggtcttc ttttacattt tctcattgaa tttccgccct
    52201 cccgaacgga tggacgtttt ccagcgagtg gtgatcgaaa ccacggcccc cctgatcaag
    52261 gccctccgaa atgtgttcat ttcaacggaa gagaccatca aagaatacgt ctggctgcgg
    52321 cagacccgcc gggaaaacga gcagcttcga agtcgcgtgg cttccctgga agaggagatc
    52381 acccattatc gggaagccta tgtggaaaat ctccgcctaa ggcgcctcct ggatttcaag
    52441 agtacgaccc aggcggaaat gatcgcggct caagtggtcc ttcatgattt gaccggctgg
    52501 ttccagacgg tcattgtaga taagggcttc agggataata tcgagcctga tatggccgtg
    52561 gtgagcgacg agggagtcgt cgggcgtacc ctggacattt ccgaccgcta cagccggatt
    52621 cttttgatca cggatccggg gagttccgtg gatgccctga tccagagaaa tcgggtccga
    52681 ggcattctgg tcgggaagga tgcaaacggc tgtgtgctga aattcgtgcg cagcaacctg
    52741 gatgtccagg tcggggacct gatcatttcc tcggggaaag acggaatctt ccccaaggga
    52801 cttcggttgg gcatagtgca ggggaccttc aaagatcccg tggacctctt ccaaaaggtg
    52861 gaagtcaagc ctctcgtgcg gctgagcgct ctcgaagagg tgcttctctt aaagcgaggc
    52921 gcgattttgc ctgaaaacct tctgggcaac tagttgggag caccggtgaa tacgacgaac
    52981 ggcctgggtg aggatcgatc ctttgtgcag tggcaatcct ttctcaaagt gaccctctac
    53041 tcccttctct tgctggtttt ccaggcggtt tgggcgtctc gaacggcctc tgcggccttg
    53101 aaaccggacc ttctgcttcc catgatgctc cccgtggcgg tgtggtggcc gccggtggcg
    53161 agcctggggt gggccatcct ctgggggttc atggccgata ccctctcggg gaaactctgg
    53221 ggctatcatg tggggtccta cgtggtgtcg gtgtgcctgg tccacatcgc cgccgagaaa
    53281 ttcgaatttc agaacccctt ataccaaatg ttcttcgtgg ggctgtgcag tctcggccag
    53341 tccctggttc tggggttgtt cctgtttctg gaacctgcgg gttccctgct gggcgcctct
    53401 gccgggagag acctggtcgg gcgttttgtg ttgacgatga tcctgtcgcc cctgatcatc
    53461 tttcccgtct ggaaagatcg ggcctcgaat tgaagagagg caggagagga aagctttgag
    53521 gtcattgctc ctgcgtcgca accgggagaa tcgcgcccgg gaaaaagaga gcaagagagc
    53581 caaggggaat cacctgctcc tggtgaactg ggagatggcc gagacggacc tatttcagcg
    53641 acagtatttg ctcctcatcg ggctgatgct ccttatgttc gtcgtctacg gcctccggtt
    53701 gtggcatctg cagttggtgg aaggggccaa gtaccgctac caatccgaaa acaaccgcat
    53761 ccggctggag gacatttcgt ctccccgggg aatcatcttc gatcgtaacg gcactcccct
    53821 ggtggaaaac cggcctgcct accatctgca gatcattcgg gaggacgtta gggatctgga
    53881 ccaaaccatt cgaacgcttg ccggtctgtg caatcggagc ccggaagagt ttttcggcat
    53941 cctggaagcc cacaagtccg tgcccaagtt tctgcccgtt cgtctcctgg cggacctgga
    54001 tcgggactgc ctggctcaaa tcgaggcccg gcgaatccgg ttgccgggag tgtccatcca
    54061 gctggaaccc aagagagagt accgatggaa cgggaccgcc gcccatctga tcgggtacct
    54121 gagcgagatc accgaggcgg aactgaaaag cgggacctac tcgggatact atcccgggga
    54181 ggatatcggc aagttcgggg tggaaggcgc cttcgagaag taccttcacg ggaagagggg
    54241 cgggcgtcag gtggaggtgg atgccatcgg cagaagaagg cggctcctgg acgaagtcct
    54301 tcccattccc ggaaggaatc tgtggttgac catcgacatc gagcttcaga aggtggcgga
    54361 atcctgcctg gaaggaaaag tgggagccgt tgtcgcattg gatcccaacg acggggccgt
    54421 gctggctatg gccagcagtc ccgtgttcga tcaggagaag tttatccgcg gcctttccaa
    54481 gtcggaatgg cagactctaa gcaaagaccc gagccatccc ctgctcaatc gggccatcgg
    54541 ggcagcttac cctccgggct ccacctacaa ggcctttatg gccatagcag cactcaaaga
    54601 aggggttgtc acccccgaca cgcaccttca ctgtcccgga ttctatcagt tcgccgaccg
    54661 caagtatcga tgctggcgag accacgggca tggttccgtg gacgtggatc gggccctcgt
    54721 tcaatcgtgc gacgtatact tctaccaatg cgggatgaga ttgggggttg atcgcatcgc
    54781 ccactacgcc aatcttttcg gattgggaga gaagaccgga ctggggctgc acggggagca
    54841 tccgggcctg atcccgactt cctggtggaa aaaacaggca acgggaatcc cctggcagaa
    54901 aggagagact ttgtccatcg ccatcggcca gggatttgat ctgaccactc cgctgcagct
    54961 gacggtggga tatgccgcca tagccaacag ggggaaactc tggcagcctt acgtggttcg
    55021 tcggatcgaa gggaacagcc gggaagaaat caacgaggtc aaggggaaat taaaaagaac
    55081 gatccccatc gaacagaaat actttgaaat tgttcggaag ggactcctgg gggtcgtgga
    55141 agacgacagg ggcaccgccc atgccattcg attgaaaggg ctccccatgg cggggaaaac
    55201 cggtacggct caggtggttc gtctggggga aaacgtcaat cggaaggccg ccgccagggc
    55261 tgccaaggaa aaggaaaaag accacgcgtg gttcgtaggc tacggtccgg cggaccatcc
    55321 ccaaattgtg gtttgtgcct tcgtcgagca tggagggcac ggttccagta cggcggctcc
    55381 cctcgtgcaa aaggtcattg ccgcctacct gtcggaccgt tccgcggggg cgggttcatg
    55441 aaagaagggg gcgagagtgt gtcccgacgg attcagggcc tcattggcgg ggccccggat
    55501 cgcattctcg tgccggaaag agcatgaccg tcatgatgga tagacgtttg atcgaaaact
    55561 tcgattggtc catcgtgctg atcctgttgg gagtgatcac cgtcggtctc ttgagcctct
    55621 acagcgccct ctacccccaa attcaagctc atcccaccca aaacctcttc atcaagcagc
    55681 tggtgtggat ttctctgggg tttgcgggac ttctctgcat tctctttctg gattaccaga
    55741 ggctccgatc catcagcctg tggatctacc tgagtgcagt ggtcatgctg gtggcggtcc
    55801 tggttgtggg caaagaggta aacggggcca agaggtggct ggaaattgtc gggtttcagt
    55861 ttcagccttc ggaattcatg aagatcgcca tcatcctaca cctggccgcc tatttctctt
    55921 cccaggagat ttccccgtac cccagcttca agaagctcct ttatccgctg cttgtcaccg
    55981 ccgtccccgt cctcctggtc ctggcggaac ccgacctggg gaccgccatc ctcctggtga
    56041 ccatttccgg aaccgtgatc ttcttcatgg ggattcggct gaggtacttg attacctgct
    56101 tcctgctggt ggcgcccatg atctggccca tctgggaaaa ggttctgaag ccctaccaga
    56161 aaaagcgaat cctgatcctc atccggcccg acttggatcc tttgggtgcc ggctaccata
    56221 ttcgacaatc gaagattgcc atcggttccg gcatgtcgtg gggaaagggc ttcctgaacg
    56281 ggacccaaac caagctccat tttcttcccg aaaaacacac ggacttcatt ttctccgtgt
    56341 gggccgagga atggggtttt gccggctgcc tgaccctgct cgtcctcttt gccgccctca
    56401 tcgtcctttc cttccgggtt gcccgcaggt ccaaggaccg atacggttcc ctgctggtgg
    56461 tgggaatgac gtccatgatt ttgtggcagg ctctgatcaa tatggggatg gtcatcggag
    56521 tcttgcccgt tgtgggaatc accctgccct ttgtgagcta cggaggttcc tctctcatca
    56581 ccctctgcat tgccgtcggg ttcatcgaaa atgtgagcat gcgccggtac gtttttcaga
    56641 cccgctgagg gttggagagc cgcgtccgag gctcatggac ccattgtgat ttttattact
    56701 tttttgcttg cgcacattgt catcttgtgc taacaacctc ccccatgttg cactccaccg
    56761 ctgcacgtaa aagcatctat ggtggatggt cccgtccatg catggaaaag ctccttgtgc
    56821 agggtctcac acggcgtttc ataagagatc atcgagggta ggacgcttgg ctttaggcga
    56881 ggaaggggaa gaatgatcag catcaacgag actcttttgt tccaaatgat caatgtcctg
    56941 atcctggttt ttctaatgaa cctggttttg taccggccca tacggaggat cgtggcccag
    57001 aggaagcaac tcgtccaaga gcaggaaggc gctattgaac gggccgagtc cgaggcgacg
    57061 gccaccgttc aagagttcga tgcgaggatt caagaggccc gcaagaccgg aaggcagagg
    57121 atccaggagc tcaagaacga ggcctacgag tatgaaaaag gcctgcttca gcaggcaacg
    57181 gagagcgcat ccaagcagtt gggggaggtt cgcgccaagg ttgccaagga tatcggaacg
    57241 gctcgggacc agctcaagaa gcaagtcaaa gctttatcca ccgatctggc acaaaaaatc
    57301 ctgggaagaa gtctttaaat tctggaaaac atgaggggga ctaggatgaa ggggagcata
    57361 cgaacacttc tcctggccgc aggcttgctc gttgccttgg gcggtgcggc atgggcgtcc
    57421 ggaggaggcg gcggagaagg ccatgggttg aactggaccg atttcgcatt gcggttccta
    57481 aatttcgcca tcttggtggc cattctcgcc aagttgctaa agaaacccat tgccggtttt
    57541 ttctcatcgc gtcgggaaag cataaagagc cttttggccg aattggagct caagcgtaaa
    57601 gaggccgagc agaagacggc cgagtacaag gctcggctgg cggccctcga aggggagacc
    57661 aagaagattg tcgatgaatt gatcgccgag gggcagctgg aacgccagaa gattgtggag
    57721 ggtgcgcaca gacaggccga atacatacgg cagcaagccc aattggccat ccagcaggag
    57781 atcaaagctg ccagagagag tttgcaggag gagatcgccg agctgtccgt ggctgcggcg
    57841 gaggagatcc tgcgcaaaaa gatgaaggcc gacgatcagg aacgtttggt gcgtgaattc
    57901 atgacccagg tggtggaggc gaagtgaaaa acttagtcat tgcaaaacgc tatgcgaaag
    57961 ccttgatgag tctggctcag gcggaccagg cggtggaatc gtacgggctg gagctggatc
    58021 agctgctgca tctcatcggg gaacaaccga ctctggggga tgtcctccaa aaccctcttt
    58081 accctatggc cgcgagaaag tctttgttcc aaagcgttgc caaggcagcg gggatctcac
    58141 ccgtaatgac gagttttgtg aacctgctca tcgaaaagaa gcgggtgcag cacctgcccg
    58201 agatcgccca gtactaccgc aaattgatgg acgaacacgc caatatcgca cgagccaaac
    58261 tcgctgctgc aacggtcctg gcagaggaca ccatcggaca gattgcgcaa acgctggaaa
    58321 aattgacggg gaagaaagtg atcgtcgagt ttgagcacga tcccgccctc attggagggg
    58381 tgacggccca gatcggcgac atggttttgg atgggagcgt aaggcgacag cttctcaatt
    58441 ttaaggaaac tttgaagagg ggtgcggtgg gttaaatgga aatcagagcc gaagagatca
    58501 gccaaattat cagggagcag atcaaggact acgagaagaa ggttgagctc agcgagacgg
    58561 gacgcgttct ctccgtcggc gacgggatcg cccgggtata cggggtggag aaatgcatgt
    58621 ccatggaatt gctggagttc cctactcccc atgggactat ttatgggctg gccctcaacc
    58681 tcgaggagga caacgtgggt gtggccatca tgggggaaga catccacatc aaggagggaa
    58741 tggaggtccg gcgcacgggg cgcatcgcgg aagtgcccgt gggagatgcg gtgctggggc
    58801 gcgttgtgga cccggtgggg aatcccatgg atggaaaggg tcccgtggaa gccgccgagt
    58861 tttcccggat tgagcggatc gcaccggggg ttatcgcacg gcagtcggtg aacgagccca
    58921 tgtacacagg gctcaaggcc atcgacgcca tgacgcccat tgggcgcgga cagcgggagc
    58981 tcatcatcgg cgaccggcag atcggaaaga ccgccattgc cgtggatgcc atcatcaacc
    59041 agaaagacag cggcatcatc tgtatctatg tcgccgttgg gcaaaagaag tccacggtgg
    59101 cccaggtggt ggaaaccctg cggaaacacg gcgccatgga ctacaccatt gtcgtggcgg
    59161 ccaatgccag cgacccggcg gccctccagt acattgctcc ctacgcggga tgtgccatgg
    59221 gtgagtatta ccgggatcgc gggcgccacg cgctcat
//
LOCUS       DAOOVC010000009        58141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_314_length_58141_cov_6.450522, whole genome shotgun sequence.
ACCESSION   DAOOVC010000009 DAOOVC010000000
VERSION     DAOOVC010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 58141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58141
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_58141_cov_6.450522"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..1188
                     /locus_tag="PLM79_02340"
     CDS             415..1188
                     /locus_tag="PLM79_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HOV85170.1"
                     /translation="MKAQPDSSSGNLWFRVGWLILFVCIWAGVPKAGFGSDVARAEVE
                     VQEKEPFEDRRVVVPDPLESWNRAVFSFNDRFYFWFAKPVAQVYGAVLPEGLRICVRN
                     SFDNLAMPVRFVNNVLQGKLQPAGVEIGRFVINSTIGFGGFFEIAARDFDLQPYDEDS
                     GQTLGFYGMPGVFYINWPFLGPSTFRDSLGRAGDYFLNPLHYLSPDIYSSAGLRVGKE
                     FNEISLRIGQYEDFKKSAVDPYVSMRDAYIQHRAGEIRR"
     gene            complement(1197..2726)
                     /gene="cobA"
                     /locus_tag="PLM79_02345"
     CDS             complement(1197..2726)
                     /gene="cobA"
                     /locus_tag="PLM79_02345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOV85171.1"
                     /translation="MRGSQVSGGKVYLVGAGPGDPGLLTLKGREVLSRADVVVYDYLA
                     NEELLRWAPPTAQRIYVGKKGGDHTVSQEDLNRLLVEKGRENLVVRLKGGDPFVFGRG
                     GEEAQMLAGAGIPFEVVPGVTAAVAVPAYAGIPLSHRDYTASMAFVTGHERDDKEDSK
                     IEWAKLSTAVGTLVFFMGVKNLPEICRRLVENGRPSTTPVAAIRWGTLPEQQTVTGTL
                     DDIVEKVRQAQLKPPAILIVGEVVRLREELNWFERRPLFGRTVLVTRAREQASDFKTR
                     LEELGACCIEFPTIDIRPPASWEPLDQAIGTLAEYDWVMFTSVNGVHYFMERLWTAGL
                     DARALGKARLAAIGPRTADALRANGLRPDLVPREYRAESILEGLSSVGVKGKSFLIPR
                     ALVARDVLPETLRREGARVNVVPAYETVLPSDRRTQVLDAFRARRIHCVTFTSSSTVS
                     NFFQLCPREEVLPWMKEVDIACIGPITAETAREYGLRTNIMPGEYTIAALTEAILAHY
                     R"
     gene            complement(2947..3882)
                     /gene="hemC"
                     /locus_tag="PLM79_02350"
     CDS             complement(2947..3882)
                     /gene="hemC"
                     /locus_tag="PLM79_02350"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOV85172.1"
                     /translation="MARTRLRIGTRGSLLALRQSENVKRDLESLWPDLQVELRIIKTT
                     GDKILDVPLAKVGGKGLFVKEIEDALLAGEVDLAVHSIKDVPSVLPEGLEIGAVPRRE
                     DPRDVLVLGKGKTLEDLPPGAAVGTSSLRRGAQLRRERPDIEVLNLRGNLDTRLRKLR
                     EGRYDAVVLAAAGLHRMGWEDQITLYLDPSRFIPAVGQGAIGIEVRKDDPRVAARIRP
                     LHDEATSLAIRAERSLLKELEGGCQVPIGGHARIENGRIRLSGLVASLDGMEVHVISA
                     EASIEEAEALGRRLAGELLEAGARRILDEIYRNPG"
     gene            complement(3915..4148)
                     /locus_tag="PLM79_02355"
     CDS             complement(3915..4148)
                     /locus_tag="PLM79_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV85173.1"
                     /translation="MPIFEYECCRCCERFERLVFGSDEAVTCPKCESTEVQRLLSVFG
                     FKSGGDKGAASSRMGSGGSGCAGCTSGSCSSCH"
     gene            complement(4274..4648)
                     /locus_tag="PLM79_02360"
     CDS             complement(4274..4648)
                     /locus_tag="PLM79_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HOV85174.1"
                     /translation="MGKCIGIRAGSVRLTGELNDSPTACAIGAALPLKAVARTWGDEI
                     YFPIPVSCPLDQTAVETVEVGDLGYWPAGKAFCIFFGPTPISRGEEIRPASAVNLIGK
                     VLGDPAAFKEVQEGDPVEIDGE"
     gene            complement(4641..5624)
                     /locus_tag="PLM79_02365"
     CDS             complement(4641..5624)
                     /locus_tag="PLM79_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85175.1"
                     /translation="MTPEKKNASVEPLPLDGLRTTNLADRPSKVCMEDFGKPWNVGGS
                     LCDFLEGLPHILAAGDLRRAADAVATAVRNRRTVLLGMGAHPIKVGLNPVLIDAMEKG
                     LFRGLAMNGACVVHDVEVALAGKTSEDVSAHLDRGLFGAVRETSELIHAAVAAGHRRG
                     GIGLGAAVGEFLLQNRAPFARSSLLASASRLGIPVTVHVAVGTDIVHMHAAMDGAATG
                     ALSHYDFRVFCRLVSALKEGVYLNLGSAVILPEVFLKAVSVARNLGFDLDGLTTINMD
                     FQKHYRTQVNVVDRPTCGSGRGLVLIGHHEIMFPLLMAAVAERLSSRGDHG"
     gene            complement(5621..6199)
                     /locus_tag="PLM79_02370"
     CDS             complement(5621..6199)
                     /locus_tag="PLM79_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOV85176.1"
                     /translation="METTVAEAFRESIRLKEELLGKETGTILRMAEVLSLAFRRGRRL
                     FLFGNGGSAADAQHIAAEFVNRFQKERPALPAMALTVDTSVLTSISNDLHFEKVFVRQ
                     LQAFARAGDVALGISTSGTSPNVLEALEWAEDRDLCTLGLAGVAPCPMDRHCRHVLHV
                     PSTVAARIQECHITVGHILCELVEDELYGGRP"
     gene            6470..7165
                     /locus_tag="PLM79_02375"
     CDS             6470..7165
                     /locus_tag="PLM79_02375"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV85177.1"
                     /translation="MNSARPNNGNGKIRSIVFDFDGTLAELLLDFALMKSNLGTLVEK
                     YLPGPVAPPSLPALEWLEELATAIGHRDGTAAEDFRREAHRMISEMEMEAAHHGRLFS
                     FTRPLLTELRQLGLSAAIITRNCERAVRLVFPDLEDYCRAFLARDHVPLVKPHPDHLL
                     RALDAMGSDSATSMMVGDHPLDIRTGKNAGILTAGVATGKTTPGELSRSGAHWTANNC
                     MDLFRELKAAGWI"
     gene            7202..8059
                     /locus_tag="PLM79_02380"
     CDS             7202..8059
                     /locus_tag="PLM79_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HOV85178.1"
                     /translation="MDLEIFQGPIRTRRAILSFTGWPDAGETIQHTLKELERGFPCEL
                     VARLDMDGFWHTESSRPQVSIQHGRIQNLEWPSYYFFLSNFASSEPVLLGTGPEPGFR
                     WRAFTREFLQLLTDWGCRQIFLLGSLFDQIFHDEILLSSVVQDPAGFNLVRELGCQRV
                     EYKGPAAIQSSIMENAHEAGIQCISIWAHIPFYLSGPHQPAMAQCLQILGRLLGLEID
                     TRPALQGWEEKVQEIETLIQQDADLRQILDGMRKNPDSRKPRSVLVPSKVIQLDEFLR
                     RRQPTSEPE"
     gene            8091..8615
                     /locus_tag="PLM79_02385"
     CDS             8091..8615
                     /locus_tag="PLM79_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85179.1"
                     /translation="MVRSGLSPEMTAFLDYTVSHFEEIARRNRFPENNQVEHEEGRCV
                     VCRPHLVAMEPFAAYLEVVARSVIVRRPTLDQGLVDEINHDSILAGLPLRVSVESILA
                     GEGESLRGWSRWVREALDTGLGLLSIHSPTSREFSLEEAEQKGFGGLIEEMVREIMAL
                     QKRNRTGDSRSLAG"
     gene            complement(8711..8911)
                     /locus_tag="PLM79_02390"
     CDS             complement(8711..8911)
                     /locus_tag="PLM79_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOV85180.1"
                     /translation="MPEGRVKWFNDKKGYGFIETKDQGDVFVHHTAIVADGFRSLSEG
                     DRVNFEVEHGPKGPQAVKVIKL"
     gene            complement(9313..10221)
                     /locus_tag="PLM79_02395"
     CDS             complement(9313..10221)
                     /locus_tag="PLM79_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HOV85181.1"
                     /translation="MRVFITGGTGFVGTYLVKRLVQEGHEVSVLSRSPGKESGELGRV
                     HYVQGDSTQAGAWQEAVPANDLLINLAGASIFTYWTEKAKNAVLESRIRTTRNLVDAL
                     SGAEGKTTLLSASAVGYYGGRLDDTLLTEDSPPGKDFLARVAMAWEAEAGRAEHLGVR
                     VVLCRFGIVMGRGGGALKQMASMFQYFLGSRLGSGKQWFPWIHLEDLYRAMTFLAGRP
                     DISGPVNCVAPNPVRNAELTRTLARVLRKPILLPPAPAVALRLVLGEFGDLLLKGQRA
                     HPQRLLREGFRFEFPLFEEAVRDLLS"
     gene            complement(10279..11046)
                     /locus_tag="PLM79_02400"
     CDS             complement(10279..11046)
                     /locus_tag="PLM79_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85182.1"
                     /translation="MSSLVLPARPSRLGTLKAKIEACLRSENLERELEALDAPPKAVL
                     NALLAFLCSGDEFLKWKSVEAVGFQMRKLADRDMESARDMLRRFMWNLNDESGGIGWG
                     IPEAMGETMAVHAALAAEYLCILLSYVREDGNRLENPLLERGALWGLGRVAHAKPELL
                     RDCFSCFIPYLDSPDPFHRGLGAWILGALGAGEARRSLDPLTTDSSEIRTYLHGKLWS
                     GPVSDLAREALAALDSTAPGTGHPLREEGKEEGRPSS"
     gene            complement(11049..11243)
                     /locus_tag="PLM79_02405"
     CDS             complement(11049..11243)
                     /locus_tag="PLM79_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOV85183.1"
                     /translation="MSKSLTVDMGECVGCDACVELCPRVFRRSPLGYIEVKEEKPQED
                     DCIWEVINCCPSGCIRWEED"
     gene            11632..13023
                     /locus_tag="PLM79_02410"
     CDS             11632..13023
                     /locus_tag="PLM79_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOV85184.1"
                     /translation="MANVQTVEKDLTREDTAESFEEMQPGLIEWKSIWMPMLFVVGMF
                     LAAFYLPVESKRFTNAVLESLALVKWYAREHVLLCLVPAFFIAGAISCFVSQAAVMRY
                     LGPTANKAVAYGVSSVSGTILAVCSCTVLPIFAGIWKRGAGLGPAIAFLYSGPAVNIL
                     AIVLTARILGLPLGIARAVGAVLFSVLIGLLMHYFFRREEEQKVQAAIHMPPVEVERP
                     LWQNVVYFGTMVGLLICATWGKPSEPVGFWNAVYEIKWWLTGAFAVALGIELHVWFRV
                     KTAKVVAAAVVVAALAFSFPQEPLIAFAAGIVALTVLITTTRGEAQEWFLSTWGFTRQ
                     ITPLLIGGILAAGLLLGQPGTEGIIPSQWVSNLVGGNSLGANFFSAVVGAFMYFATLT
                     EVPILQGLLGSGMGNGPALALLLAGPALSLPNMLVIRSVMGTKKTVVYVSLVVVMSTL
                     CGLIFGHFFPNEP"
     gene            13080..13313
                     /locus_tag="PLM79_02415"
     CDS             13080..13313
                     /locus_tag="PLM79_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV85185.1"
                     /translation="MEIKVLGPGCPKCHQTEKVVKEAVAESGVDAKIDKVTNILEIGE
                     YGVFGTPAVVVDGEVKSVGKIPTKAEVLKWIQK"
     gene            13341..13724
                     /locus_tag="PLM79_02420"
     CDS             13341..13724
                     /locus_tag="PLM79_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOV85186.1"
                     /translation="MTQECCTPGGNLMILTCSGGSNVGQLSNQAAVELTREGFGKMFC
                     LAGIGGRLKGFVQSAKDVPRMVVVDGCSLACAKACLDQAQVPVKNYVVLTDLGIEKNK
                     DFDLKREEIDRVKQAVKAACATSDG"
     gene            13918..14439
                     /locus_tag="PLM79_02425"
     CDS             13918..14439
                     /locus_tag="PLM79_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85187.1"
                     /translation="MLLQGYRKEIVRPECRREAESLHCIAHLDQDIGEVLPYLNTVLG
                     GFQYTRDPPSVSFKAHGKLIAVHPRMICVNALKDETEGNRILEWLKGEINDAWERRAE
                     IRPSYEGAPKPRLLEILRLLPKTNCRECGQPTCTVFAAQVAEGGKGPESCPAISPENR
                     ERLEAYLGRFRFE"
     gene            14475..14927
                     /locus_tag="PLM79_02430"
     CDS             14475..14927
                     /locus_tag="PLM79_02430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOV85188.1"
                     /translation="MAGEHGKLKILFLCTGNSCRSQMAEGWARHLKNDRIEAYSGGVD
                     PHPVDPRAVRAMSEAGVDISSQVSKDVDTFESIEFDYVITLCEKANRSCPVFPAKTRL
                     VHVAFDDPPRLAAEAHSEEEAMAPYRRVRDEIREFVEKLPEILTEEDR"
     gene            14945..16114
                     /gene="arsB"
                     /locus_tag="PLM79_02435"
     CDS             14945..16114
                     /gene="arsB"
                     /locus_tag="PLM79_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOV85189.1"
                     /translation="MEDPGPKKLSFLDRFLTVWIVSAMLVGVGWGYFFPGVRGIINLF
                     QVGTTNIPIAVGLILMMYPPLARVRYEELHRVFRNRKVLGLSLVMNWIVGPILMFVLA
                     VIFLADYPAYAVGLILVGLARCIAMVIVWNELACGNREYCAGLVAFNAIFQMLLYAVY
                     AYFFIAVLPKWLGLGFATEIQVSIPDVVKTVMIYLGIPFFGGLATRLILIPAKGKEWY
                     ETQFIPTISPITLIALLFTIVVMFSLKGEYIVTLPLDVVRVAVPLAIYFVVMFFLTFY
                     LSHRLRADYPTSASLSFTSASNDFELAIAVAIAVFGLESGQAFATVIGPLLEVPILIS
                     LVNVALRFKQKYFAEDTCTVCGVAATNTVLVQCRQGEKEVRVCTDCARGSTPSGN"
     gene            complement(16146..16919)
                     /locus_tag="PLM79_02440"
     CDS             complement(16146..16919)
                     /locus_tag="PLM79_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OS_HP3 family (seleno)protein"
                     /protein_id="HOV85190.1"
                     /translation="MVTRFDRAIPPGGEGTVTLKVILKGYKGNIKKNATIESNDPRNP
                     RTALTMQGKVRTLIDVRPADTIAFRGPARSMGESTLDLAAALQPFRITRVESNLEDKI
                     AHQLVTVEDGKLYRLQVTNLARQGHYSGFLRLHTDFERKSEILIRVNGSIEGEISVKP
                     GALLVGKLSAQQPVRKGRVVVASNSGKPFKIINLVHDEKLIRVTQSPLPDQQGVSLEI
                     VPRMENVPPGAPQRTVLSIETDMASEGKQEVQVQVFHAP"
     gene            complement(16926..17186)
                     /locus_tag="PLM79_02445"
     CDS             complement(16926..17186)
                     /locus_tag="PLM79_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85191.1"
                     /translation="MARSLPFRGFIAVLSGLLWGLPSGTFPAFSQESSFSPGNPGEAP
                     RITVAETKFEFGEVDEGSEISHDFIVMNRGKGELRITKVSRD"
     gene            complement(17287..17997)
                     /locus_tag="PLM79_02450"
     CDS             complement(17287..17997)
                     /locus_tag="PLM79_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV85192.1"
                     /translation="MLDQFFLTINDWMMGGVLIAALGCLLWGMVSVALSPCHLASIPL
                     IVGYVAGQDKALKAKDAAWYAIVFSVGLFISIALVGIICSLLGRMLGEIGPYWTILVG
                     AVLIWVALDMWGISACSMSGSLLGRIKIKGITGAFVLGLAYGILSGSCTFGFIAPILA
                     IITIQQKILIGIAYIVLFGIGHCIPIAIAGSSTAKVRRLLESGSFHESSQWFRKLAGT
                     AVGILGLYFVVRPFSDVS"
     gene            complement(18169..18576)
                     /locus_tag="PLM79_02455"
     CDS             complement(18169..18576)
                     /locus_tag="PLM79_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV85193.1"
                     /translation="MKSNLRFQTTTGLMFLLILLGLFTLSCSAEKSAGEIPEIPAKGK
                     VTMVDIGANECIPCKMMAPIMEELESEYHHTGKAAIYFIDVWKNREQGRRFGIRSIPT
                     QIFYDKDGNEVQRHEGFMQKKDIVATLRKLRVE"
     gene            complement(18681..18980)
                     /locus_tag="PLM79_02460"
     CDS             complement(18681..18980)
                     /locus_tag="PLM79_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85194.1"
                     /translation="MVLYVAAHAQTQEAFFEARKIYMPQSTDGLGSTMVLGPDTKLGL
                     IRDTSIQPFGSREEVFEIPVPAEGGEVSVHVDLSYQPRPGDVIPVHSVVRKVALR"
     gene            complement(18996..19889)
                     /gene="extKL"
                     /locus_tag="PLM79_02465"
     CDS             complement(18996..19889)
                     /gene="extKL"
                     /locus_tag="PLM79_02465"
                     /inference="COORDINATES: protein motif:HMM:NF040886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome ExtKL"
                     /protein_id="HOV85195.1"
                     /translation="MADRGFTKAGRSGAVFFLVLAILPWIAHGGTIHPPEIHSLSGLI
                     ERFDSSGCRECHEAIYRQWESSHHARPLMGLNDWIFLSKYLKEGALAVRSAREATRAN
                     FPCAKCHLPQLSLATDEAVKELAAAILKDDKETVRKLGITCLVCHQEKANVHHRPETD
                     ALYGSRELRDHGGKYSTVRQSPVMKDPVFCGQCHGQGPNLDTFPPVQCATLYGSYLHA
                     YIPAGGTQTCQDCHMPHKDHSVRPNFNDRDETSARLAAALPMEVQVLAYTFHPAENVK
                     TPMIVVRTRITSKAGHRVPDG"
     gene            complement(19961..20527)
                     /locus_tag="PLM79_02470"
     CDS             complement(19961..20527)
                     /locus_tag="PLM79_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85196.1"
                     /translation="MFRAFVLLFLAVGTLGGCVTIPQKSPQSEARIIEANAALAVVAD
                     ETDQLFRESLDLRREVKSLYEHPGWPDMKKIIETMGTAEESENEASGEVVDAAARWSR
                     DWNEPWEILFARYLVLVKRCSILEAKRLALQSRLTGVQAKYIGAAALEYADGRVSRGK
                     SLEEVAETLNKSREELDVSNLNALGLCE"
     gene            complement(20900..21730)
                     /locus_tag="PLM79_02475"
     CDS             complement(20900..21730)
                     /locus_tag="PLM79_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HOV85197.1"
                     /translation="MKAEEIKNAVKSSYAKVALSNLPCCGTGKSCCGSPNLAEELSRK
                     VGYGDEELEAVPEGANLGLGCGNPVALASIEEGETVLDLGSGAGFDCFIAASKVGNTG
                     KVIGVDMTPEMVEKARRNAREGNFDNVEFRLGEIENLPVSENEVEVVISNCVINLSPD
                     KEKVFREAFRVLKPGGRLMVSDIVLLKELPAAIRKSVGAYVGCIAGAVMKDEYLAAVE
                     AAGFQEIRVLGQDTYPLELMAADPIAQPICADLNLSREEAKELSSTIASIKVSAVKGA
                     "
     gene            complement(21761..22042)
                     /locus_tag="PLM79_02480"
     CDS             complement(21761..22042)
                     /locus_tag="PLM79_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HOV85198.1"
                     /translation="MPRKSKKEPCCDPETTGKDCCRIESLISVDERGQMVLPKELRDR
                     AKIRAGDKLALISWQKDGEVCCLTLIKADALAGRVQEFLGPVLRSLSAD"
     gene            complement(22430..23374)
                     /locus_tag="PLM79_02485"
     CDS             complement(22430..23374)
                     /locus_tag="PLM79_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85199.1"
                     /translation="MTNEEKAEELLYDVIILGAGPAGLSAAIYTIRKAMKTLILSKDV
                     GGQLTWTSDVDNYLGFSQVNALELVAKFEEHVRRFNVERAIGVDVAEVFPAGPVKRVV
                     TRDGRSYRGKTIIIATGGRHKPLNIPGEREYVGKGVSYCSTCDAPLYAGADVAVIGGG
                     NSALGAAIDLMGIANKIHLIFLMPELTGDPVYQARVRQSEKVVILPQCEPTRIFGRDY
                     VEGIEYRGMQSGEIKTLAVEGVFIEIGTLPNSGLFSHILTTNEKGEILVDHECRTGLA
                     GVFACGDVTNVPFKQVVVAVGEGSKAALSAYNYLISRA"
     gene            complement(23391..23639)
                     /locus_tag="PLM79_02490"
     CDS             complement(23391..23639)
                     /locus_tag="PLM79_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HOV85200.1"
                     /translation="MAKKVKIYTTAACHYCRQAKEYLTEKGVEFEAVDVSSDPQALQE
                     MKKVSGGARSVPVISIGEKVIIGFEPELLDEALKGDQP"
     gene            complement(23774..23932)
                     /locus_tag="PLM79_02495"
     CDS             complement(23774..23932)
                     /locus_tag="PLM79_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOV85201.1"
                     /translation="MDRYVCQICGYVYDPAEGDPDSGIAPGTPFEKLPDDWTCPICGA
                     SKADFKKE"
     gene            complement(24167..26641)
                     /locus_tag="PLM79_02500"
     CDS             complement(24167..26641)
                     /locus_tag="PLM79_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOV85202.1"
                     /translation="MVKRSLHVRGMSCAACVARVEKGLGSMEGVRSADVNFATGKVSL
                     EYDPQLVDLETLKGRVQELGYEPVELPSDGEQGPRKVTLLVGGMTCAACVRRVEQALK
                     SAKGVADAVVNLATSRATVTYDPESVGLSDLRGIIQRAGYDFLGLAEEFRKDPIEAAQ
                     QEERRELKIKLGVGAFLNVWIMMGSMQEWFPFLSGIPLRVMSFFLLVLTTPVVFWVGR
                     RFLYGALKAARQKTADMNTLVALGSLSAYVYSTLGVFRPDLVDPLGHGPHLYFDSAAM
                     IVTLVLLGRFLEIGARGRTSEAIRRLMRLTPKTAAVLRDGIEVDLPLEEVRRGDVVLV
                     RPGGSVPTDGILLTGGSTVNESMLTGESLPVTKEVGDKVFAGTLNQSGSFSYRATRIG
                     AETVLAQIIRLVEDAQGSKAPIQRFADKVASIFVPAVMAVGVVTMLVWYFADPNPGFS
                     RALLNFISVLIIACPCAMGLATPTAVMVGTGIGAENGILIKGGESLERAHELTTIVFD
                     KTGTLTQGTPRVVEVRTLPGVDPSQLLSWAISVENLSEHPLARAIVDWGRARGIQPLP
                     VESFEGLSGLGARGIVEGRSVALGNQRLLDQWGISLDALEDPANRLAEEGKTCVFLAV
                     DQRIQGVIALADAPRETAGPAVSALKRMGLHVAMITGDRPETARAVARELGIDRVLAG
                     VLPGDKAQEIRRLQEQGEVVAMVGDGINDAPALAVADVGIAVGAGTDVALEASDITLI
                     QNDLRMVASAIRLSSLTMRVIKQNLFWAFFYNVLGIPVAAGALYPFFGVLLNPMFAAA
                     AMALSSVSVVTNALRLRRVWRKRNLP"
     gene            complement(26634..26831)
                     /locus_tag="PLM79_02505"
     CDS             complement(26634..26831)
                     /locus_tag="PLM79_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOV85203.1"
                     /translation="MKSVKIKGMSCQHCVMAVTKALNEVAGIKDLEVDLSRGEATFEE
                     SEPVDMEWVRNQIRKAGYEVG"
     gene            complement(27165..29234)
                     /locus_tag="PLM79_02510"
     CDS             complement(27165..29234)
                     /locus_tag="PLM79_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOV85204.1"
                     /translation="MPNRLIHEKSPYLLQHAHNPVDWYPWGEAALLKAGRENKPIFLS
                     IGYATCHWCHVMAHESFEDREVAALLNEHFVSIKVDREERPDLDNIYMSACQALNRSG
                     GWPLSVFMTPGQKPFFAGTYFPKHGRMGMPGFVDILSQVVHLWEAQPDRVRQAGEEVT
                     RAIQPREFSGSSAVPKEEVLARAQQQLKEIHDVQWGGFGGAPKFPTPHHLTFLLRRHG
                     QGPRSDALDIVERTLHSMRRGGIFDQIGLGFHRYSVDQRWLVPHFEKMLYDQAMLSMA
                     YAEAYQLTRNEFYARVFREIFEYVLGDMTDPRGGFHSAEDADSEGQEGKFYVWTPDEV
                     LAALGKEDGDLFCRFYGITPGGNFEEGSSIPHIPKPASVFSEMIGMRVHELEGILESG
                     RQKLLAAREQRIHPLKDDKILTSWNGLMIAALAKGFQASGNSAYLDAAARAADFVLTV
                     LRDDSGRLYRRYRLGEVAQRAYLDDHAFLVWGLIELYEASFDVRYLEEAVNLTRQTLD
                     LFGDEREGGFFFTGKDAESLIVRDKPIYDGAIPSGNAAALLNLLRLGRMTGNTDFDQA
                     AEGCLRRFGPLVEEYPSGYTQFLIGLDFALGPTREMVVVGDSREAQTGEMIRTLHRAF
                     RPRSVLMFRATDNDDGRLTKIAPFVEPLRSLAGKTAVFACENYACRTPVTDVRDLEAS
                     LGSRPPT"
     gene            complement(29656..29844)
                     /locus_tag="PLM79_02515"
     CDS             complement(29656..29844)
                     /locus_tag="PLM79_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85205.1"
                     /translation="MAKKQRHLVKGQCPQCACGDVSFIPPETMKDKYIGPEQEIEILC
                     PSCGTKHKGTLVLEEEKD"
     gene            complement(29924..30529)
                     /locus_tag="PLM79_02520"
     CDS             complement(29924..30529)
                     /locus_tag="PLM79_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOV85206.1"
                     /translation="MKIDSKPARFLVALVAVFCWNTALSHAVSEKFEGKIYSGGPLKP
                     LDSRTQLKVGDPAPDFTLPSLSGEKVTLSDFLGKKNVVLSFVPAAWTPVCSEQWPGYN
                     LVKDYFDQYQAVILGITVDNLATLHAWTQQMGGVWFPVLSDFWPHGAVAAKYGVLRSE
                     GTSERAVFVIDKQGIIRYMDVHDINRRPNLEAMIDELRKLP"
     gene            complement(30541..31134)
                     /locus_tag="PLM79_02525"
     CDS             complement(30541..31134)
                     /locus_tag="PLM79_02525"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOV85207.1"
                     /translation="MGGFKRIGWWVLVGAVAVGMLIPAPGASSEAIGPPEEGGVLPDV
                     KLTLPDPAAHQQYLGVGAEGSFKIPQVRAELVILEIFSMYCPYCQKEAPVVNELYRLI
                     SGNPDLKDRVKIVGIGAGNTSFEVATFQKQFSVPFPLVPDNDLNLHRTFGDVRTPYFI
                     VIRMMQDGSHQVIYSKAGAFGDPQEFLNLLLSKGKRN"
     gene            complement(31620..32165)
                     /locus_tag="PLM79_02530"
     CDS             complement(31620..32165)
                     /locus_tag="PLM79_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV85208.1"
                     /translation="MDTNQDPMVTIFCTAIEMKEKKKALYEGAMKTCPDQVGIETFRM
                     LKDAEEDHARSLQAAYEELKKGSVPADACRFVPFDGEDKKSLLRRIAREHGRMPRACL
                     DDVAAIETGLSLENASISFLDKQARSAEDKGVRELLERLIGEERQHCIVLADLKFYYT
                     DPEGWFIEKGKAGLDGAGELA"
     gene            32439..32912
                     /locus_tag="PLM79_02535"
     CDS             32439..32912
                     /locus_tag="PLM79_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOV85209.1"
                     /translation="MFELTPFRKQRSVTNAPLAWDWADRLLNEFMPFRLFREMMPSLW
                     REQGALMPAFDITEAEDHLVVRADLPGIDARNLDISLAGNVLTIKGEKKDERSEQNEF
                     YHFCERQFGSFTRSFTLPSDVREEGIEATYKDGVLQVRIPKTERTEKKRIEVKTH"
     gene            complement(33109..36123)
                     /locus_tag="PLM79_02540"
     CDS             complement(33109..36123)
                     /locus_tag="PLM79_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(35932..35934),aa:Sec)
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV85210.1"
                     /translation="MNHTERKEKVGAVMVVGAGIAGMQAALDLANSGYFVYLVDRASC
                     IGGLMAQLDKTFPTNDCAMUIISPKLVEVGRHLNIRIITNAEVEAIEGEAGHFKALLK
                     SYPRFIDLEKCTGCGVCAQVCPVPAVNEFNAGLDTRAATSIRYPQAVPLVYSIDPDSC
                     VGCGLCETVCLAKAVRFDDTLRRVEVEVGAVILAGGNEVFDPSEYDTYDYARLPNVVT
                     SLEFERILSASGPFRGHLMRPYDREEPKKIAWLQCVGSRDINVAGNPYCSAVCCMYAV
                     KEAVIAKEHAHGPMDAAIFFMDMRTYGKDFDQYYERAKEEHGVRFIRSRVHSISPVGD
                     GDLEIQYVGEDGRQQREVFNLVVLSVGFRVGQELLDLANRLGIELNQYRFAKTDSFAP
                     VAASRPGIFVCGTFQGPKDIPQAVVEGSAAAASSGVLLNDVRWTVTREPSTPSQTDIR
                     GEVPRVGVFVCHCGINIGGVVNVPEVREYAKRLPFVVYAEENLYSCSQDTQTKMAEVI
                     REKGINRVVVAACTPRTHEPLFQETLVSAGLNRYLFDMANIRNQVSWVHKNDPVTATE
                     KAKDLVRMAVAKATLLEPLQDTEVPLNPVSLVIGGGIAGMMAARTLAEQGYEVHIVER
                     TAELGGAARSLFRTWRGENVQSYLYDLEQQVRANPLIHIHRSAEVVNVEGFVGNFVTT
                     IRPEKGDPFSLGHGITILATGAREFKPDDYLYGKDPRVLTHLELDARFIQGDESLKEI
                     RSAVFIQCVGSREANRPYCSRVCCTHSMESALELKRRNQDMDIYVLYRDIRTYGERED
                     LYSQARKEGVVFVRYDPENRPQVRTEGSALQVTVTDPILGRPLAIEPDLLVLATAIVP
                     SENELLSQFFKVPLNSDGFFVEAHAKLRPVDFATDGVFVCGLAHYPKAVDESIAQALA
                     ASARAMVLLTRGKVLVSGAVAETNQFLCSGCGTCVTVCPYSAASLNVHGKSEINPALC
                     KGCGLCTASCRSGAIRLHGFDDAQVFAMIESV"
     gene            complement(36622..37017)
                     /locus_tag="PLM79_02545"
                     /pseudo
     CDS             complement(36622..37017)
                     /locus_tag="PLM79_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
     gene            complement(37039..40098)
                     /locus_tag="PLM79_02550"
     CDS             complement(37039..40098)
                     /locus_tag="PLM79_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV85211.1"
                     /translation="MEDRSNTSPMTAAVLVVGGGIAGAQAALDLADSGYFVYLVEKDT
                     SLGGIMARLDKTFPTNDCSMCILSPKLVECGRHLNIQLITNARLESLEGKEGDFLATV
                     REEPRFVDTEKCIACGVCAEKCPRRVEDEFNEGLNKRKAAYIRYPQAVPLKYALDREH
                     CIYFEKGKCRACEKFCPAGAVDFHQEGRVHQLRVGSVILAPGLSALSPTAYDTYGYGV
                     HPNVITSMEFERILSASGPYQGHLLRPSDRKAPRRIAWLQCVGSRDLHHGGHGYCSGV
                     CCMYAVKEAVIAKEHAGADLDTAIFYMDMRTYGKDFERYYDRAREEVGVRFVRSRVHS
                     VRPSSPGSHELSLEYVDDAGRTHSEEFDLVVLSVGMELSADTIELAGKLGIRVDESGF
                     GSTCSFSPVTTSRPGVYVCGGYLGPKDIPHSVMEASAASAASSASLADARGTLTHEKT
                     YPPEDPIILEKPRIGVFVCHCGINIGGVVDVPAVRDYAATLPNVAYVADNLFTCSQDT
                     QGLIRQAIKDHRLNRIVVAACTPRTHEPLFQETIREAGLNRYLFDLANIRDQNSWVHM
                     ADPRKATQKAKDLVRMAVARVALLEPIEELEAETWRSALVIGGGIAGMNAALAVADQG
                     LRVYLVERQPVLGGHGRHIRSTWKGEEVGGYLNDLNHRVFAHPNIEVLTEARVESAQG
                     FVGQYTTRISVNGKSRDLNHGVVILATGAETLEPQEYLFGESERVTRWHELQDLFDRK
                     PEKLEEAEAVAFLLCVGSREPDRPYCSKVCCTSSIRQAIDLKTRKPDLDVYVLYRDIR
                     TYGLREELYTQARREGVLFIRYSRDEKPRVEASGNKLQITVRDHVLDRPVRLEVDYLN
                     LATAVIPRGLKELARIYKVPVNQDGFFLEAHMKLRPVEFSTEGIFVCGLAHCPKPIEE
                     SIAQAQAAASKATKILVQPHLKLEPTVSRIDPELCVGCGLCEKSCPFGAIRRYEVVGR
                     GYVAESIPASCKGCGVCAAACPQKAIDMLHFRHSQIVATIRAGGKKA"
     gene            complement(40198..40452)
                     /locus_tag="PLM79_02555"
     CDS             complement(40198..40452)
                     /locus_tag="PLM79_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOV85212.1"
                     /translation="MKKKVDHIFDLRGSFAPIALLQLTQELEEMKVNEIMEIIGCGPE
                     TLADLLRIVPASSFEFIHLKPAKEDSFYCICLRKRGKPQS"
     gene            40889..42283
                     /locus_tag="PLM79_02560"
     CDS             40889..42283
                     /locus_tag="PLM79_02560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV85213.1"
                     /translation="MAERALDRYWKAVVSTIRDGIIIVNPQGTIISVNKALENMTGYS
                     REELIGKKCSIMECSLFEKAREQRGEHWCVLFNSGDLTSRKCTLLRKDRTILHALKSA
                     SLLHDDHGKVIGAVETITDITELIEKDIQIAAFRRELQSEDGFQGLIGTSAPMQRVFD
                     LIANAAQSDAPVIIFGESGTGKELVSKAIHNLSARSQKPFIKVNCAALTESLLESELF
                     GHVKGAYTGAYRSRAGRFEAAQGGDIFLDEIGDLPLSTQVKLLRVLEEKVVERVGDNR
                     PIPIEVRIITATNKNLLQLVEKGLFREDFYFRINVIPIHLPPLRERVEDIPLIAESFF
                     RKIRLKSDKRAEGISADTMEVLMNYPWPGNIRELKSAFEFAFVTCQERLIQPWHLPQN
                     ISKYRGQAGRSGPKVLDSRMDMEKRRLLAALSQAGGNQSEAARILGVSRVTVWNRMKR
                     LGIAKGKIFNTGST"
     gene            42390..44030
                     /locus_tag="PLM79_02565"
     CDS             42390..44030
                     /locus_tag="PLM79_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85214.1"
                     /translation="MTKEHQSSSRSEGFVRSDLENELKAVFERLPHPVSVYLVAGRAG
                     EDVLSRAARELIRGFQGLSDRIRVTEVEPRDDLARKWKVNSIPTFLFDPEHYSIRYLG
                     VPFGEEARTLLGILILLGYRSGNLTEQSARVLQRIDTPRRIKVFVSATCPYCPEQAMN
                     AARAAMEKPDLISVEIIDIQSNQEMANRYSAFSVPQVFANEVLIAQGAQSEELFMLSL
                     EKMEQQTLFIPESDSEEVETDLLIVGGGPAGLTAGIYAVRSGLKTAIVEKGPLGGQIA
                     TTPVVENYPGFTRVPGKALVDILASHALEYVQIFPGEEVVEVRPGNPIRIRTTRRRFK
                     AGAVILATGATYRRLGVPGEMELAGRGVSYCATCDGTLFKGKKVLVVGGGNSALTEAL
                     YLSNIGVSVTLVHRRDAFRAQEHLVKNVAANRIPVLWDTEVKEIRGRERVTQAKLVNR
                     KTGETTWTDTDGVFVAIGYVPTVDLARKLGVEVTPDGFIRHDAHHRTNIPGIYSAGDV
                     EGGYKQIVTAMAQGTEAALSVYEDLTHPYWKQEGAPPG"
     gene            44173..44367
                     /locus_tag="PLM79_02570"
     CDS             44173..44367
                     /locus_tag="PLM79_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85215.1"
                     /translation="MFCVDLSAEETGVLQRVPDSYLSELKEEIAHTDRRESRQMLRGE
                     EEVVKRVIRELGSEASSEIH"
     gene            complement(44570..46708)
                     /locus_tag="PLM79_02575"
     CDS             complement(44570..46708)
                     /locus_tag="PLM79_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOV85216.1"
                     /translation="MTTLDAIFSPRSVAVVGASTKPGKVGHDIFVNILKGGYQGVLFP
                     VNPTARAIQCVKAYPTLSDVPDPVDLAILILPPRESLKTVEEAAKKGVQGIVIVSAGF
                     REVGPEGRAVEDRIAAVCKDAGMRLVGPNCLGVINPHPSVKLNASFSGRMPAFGNISF
                     ISQSGALCTAVLDFAADREFGFSKFISIGNKADVDELDLLRYLHEDKDTEVIMIYLEE
                     LRRGAEFTQAVKRITSGDRPTPVLVVKSGRTAAGAAAAASHTGSLAGSEGTYDAVFQQ
                     AGIIRADSIEELFNFAGAFASKKTPSGNRVAIVTNAGGPGIVATDMTISSGLTLARFH
                     EETVEALASHLPATANLHNPVDVIGDASIDRYQNALGAVIRDEGVDGALVILTPQSMT
                     DALGTAEAIAKIALHTAKPILCCFMGIVDVSSGVKYLQERGIPVFRFPEHAAQAYGAL
                     YRYSRWLNRQHLAQFSLTHDRDRAAQAIKRCLDQGKTRLGEADTHEILNCYGFDVLPT
                     YLAVTREEAGDFAEKIGSSVALKIVSPQILHKSDVRGVELHIQGREAAEEAFDRIMEN
                     VRNLAPAARVTGVLVQKMADPGEELILGVSRYAGFGPLLMFGLGGIFVEVFKDVIFRL
                     APIGRNEARRMVRGIKGYAVLDGYRGKPPVDGELLERLLVSLSDMALNHPEIKELDIN
                     PLVAYEKGKGAVVADSRMILEPPREDPASS"
     gene            complement(46750..47148)
                     /locus_tag="PLM79_02580"
     CDS             complement(46750..47148)
                     /locus_tag="PLM79_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOV85217.1"
                     /translation="MIDTIQLGTPDRTALLDITDLVAGKVREREVADGLCIVYVPHTT
                     AGVMINEGADPAVMQDVANTLEKLVPWKAAYLHGEGNSAAHIKSILVGGSVQVLVEGG
                     RLLLGTWERIFLCEFDGPRTRKIHVKILRD"
     gene            complement(47161..48384)
                     /locus_tag="PLM79_02585"
     CDS             complement(47161..48384)
                     /locus_tag="PLM79_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85218.1"
                     /translation="MKSTKKSELELAPEENRELEMIWDRLSVQNPRGATLHSCLQSMR
                     EMLTGREKLVAALIDRLSKRPTEVSFRAFRTLEDLVEDKKYRRLIKQAGYRFGQKGYP
                     SAQPAPEEKTVVLIPKEERKAVAHLVSGEEVFLLVSALVPEAGSSLWPLAISAYAEEN
                     FAGLSVHVTESSNRAYREYIQMLTEASDFKPAEIPIWHAARLFMEMAEYCGERGRTPE
                     ADTAKRLFQPFFEPERKPYAYSLMADVENPERFLNGIDVMGLLEKVPYSWLFFPQKDL
                     QPYWQRILDLERPVLVVPNEIQYQRTLEMVGKAAEEICTGALRRFYQRFFEEQALWFK
                     LSDREEPARSCWVVARHLAGTGSAGDNPVVRQMVILSMGHYWPEEFEKADQAGEKGEK
                     AEPFYLSESGLILPR"
     gene            complement(48540..49763)
                     /locus_tag="PLM79_02590"
     CDS             complement(48540..49763)
                     /locus_tag="PLM79_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083764016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(49080..49082),aa:Sec)
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85219.1"
                     /translation="MEPLLLTKRERRKLASQYAELCLTCGTCAGGCPVTGVDGLDVRK
                     AVRLAVLGMDQELIDSRFPWVCTLCGRCEHACPMKIDLLTLLRSARGMRARDKVPGVL
                     HKGVEMCLKTGNNVGIPKDDFVFLLQDLGAELAEELPGFEVPIDKKGAKYLFTINSKE
                     PFAEPEDMMFWWRILYAANESWTTTSENWEGVNWGLFTGDDNAMKEIVGRIVKNYKEL
                     GCEYLVLPEUGHAYYATRLGLQKWYADEGVKFVSVHDLLKMYIEEGRIKIDKSVHHEL
                     TTYHDPCNYARKSERAFGHGYYEEPRWVTQQCCENFVDMYPNRANNFCCGAGGGAWAS
                     PYVEERIFYGRTKAKQIKDTGAKLLVAPCHNCRDQIMKSLRKEYDFMDVEVKYLWELV
                     ADSLIIEPREEDGEE"
     gene            complement(50026..50868)
                     /locus_tag="PLM79_02595"
     CDS             complement(50026..50868)
                     /locus_tag="PLM79_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOV85220.1"
                     /translation="MNNPYLPYPVRIRSVVTETEDRNLKTFELVFLRPEDEARFQYMP
                     GQFAELSVAGLGEIPIGIATSPTEKGFIKFTVNKVGKVTHHLHNMSPGEVMGVRGPLG
                     NWYPWEILEGKNVVIVGGGFAFTTLRSSIVYMLHPENRARFGDISVVYGARSPGLLLY
                     KEELAEWERRKDINMHITVDSTNDPTWKYNVGFVPAVTKQKAPSSENAYAIVCGPPIM
                     IKFTLPVLTDLGFPPDRIITSLEMRMKCGIGICGRCNIGTEYVCKDGPVFTLEKLSGL
                     PNEY"
     gene            complement(50897..51946)
                     /locus_tag="PLM79_02600"
     CDS             complement(50897..51946)
                     /locus_tag="PLM79_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV85221.1"
                     /translation="MAERYISKEKFTRILDRLMSRYRVYGPVFGGMYHDFEVLKEAGD
                     ADLSFRNTRLSPKGLLHPHSERMFEYSLAKDDPEAGIVKEAAKEYPATLILGIRPCDA
                     RAFQLVELNYVTEAFQDPWWRNRRKAAVLVGLACNEPCESCFCTTAGTGPFDETGLDI
                     LLVDVGTGYVARAVSKEGGTLLGSMEGDAVPSDLTEESARLQRKAEGQLGKQFNVDDL
                     KSKTVSELFNAPFWDEVQFSCINCGTCTFLCPTCWCFDIQDEVYKERGDRIRIWDSCM
                     FPLFTHHGSGHNPRAQKLQRVRQRFMHKLKYYPEKYKDGIACVGCGRCVKHCPVNIDI
                     RHVARLMSTSCTCPA"
     gene            complement(52018..52968)
                     /locus_tag="PLM79_02605"
     CDS             complement(52018..52968)
                     /locus_tag="PLM79_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOV85222.1"
                     /translation="MRAYTERIREAAKRLLTEKRVDVVIGFRKGTIPLVNEPFLVKDP
                     KDADQLYWDGNCGVNLANYLAKRTDRVAVVAKGCDTRNIVVHLLEFQIKREQLYILGA
                     PCHGMLDRRKIAGELKGKEPTFAEESDGTVKISGREFEVTLDRKEFLQENCSICIHRN
                     PVLYDELVGDLVEEQQNVDRYVDIREIEALLPADRWQYFSKLVEPCIRCYACRNACPA
                     CYCPTCFVDESRPQWVGKSLDKTDTLTFHFLRAYHLGGRCTDCGSCERACPVGIKVRK
                     FTKKLEKDIRELYGYETGLSVDERPPLDTYRPGDPEAFIK"
     gene            complement(52969..53403)
                     /locus_tag="PLM79_02610"
     CDS             complement(52969..53403)
                     /locus_tag="PLM79_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85223.1"
                     /translation="MSEWEPKIVAFLCNWCSYGGADLAGISRLQYPANIRIIRLPCTG
                     RVNPKFILATLRHGADGVWVSGCHPGDCHYIKGNLYARRKFALLKNFLEYTGIIEPNR
                     LHFSWISSAEATKFAEVARGVVDAVRAAGPAQHFIKKQAEVV"
     gene            complement(53507..57934)
                     /locus_tag="PLM79_02615"
     CDS             complement(53507..57934)
                     /locus_tag="PLM79_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(57740..57742),aa:Sec)
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85224.1"
                     /translation="MKGNGTSKPMTGAVMVVGGGIAGMQSSLDLANSGYYVYLVEKES
                     SIGGAMAQLDKTFPTNDCAMUIISPKLVEVGRHLNIELLTLSEIREVSGEPGNFRVKV
                     LKKPRYIQPEKCTGCGDCAKVCPVNLENGFEQGMARRKATFRRYAQAIPGAFAIDKYD
                     RSPCTQACPNHVNAHAYVALTAEGRYPEAMEVILRTLPLPGVLGRICPHPCESSCRRG
                     QVDEPVSICAIKRFVADQVDVTELPVPQISRRDEKVAVIGAGPAGLTAAHFLARDGYG
                     VTVFEALPVAGGMLRVGIPDYRLPPSVLEKEVRAITRLGVEIRYNTVLGRDITLDGLF
                     ADGYKAVYLAIGAHASLKLGVPGEDAPGVIHGVDFLRQVNLKELTRVQGRVAVVGGGD
                     VAIDAARCALRLGADEVTILYRRSRREMPARDNEIEDALAEGVQIQYLTAPQEVITRD
                     GRVAGIRCIRMELGEPDQSGRRRPVPVPGSEFVVDAQYVVPAIGQAPDSLFLSSNGIS
                     LGRKGTIETDPVTFATNIPGIFAGGDAQSGPWIAIGAVAQGREAAISISRYLRGEDMR
                     EGRDGPEVSQENFLPIPKGIETRPREHMPVLDMARRKVGFDEVERGLTEEQAKSEASK
                     CLSCMACCECLQCVSACKAEAVDHGMTEELVTLDVGAIIAAPGFKPFDPRTLDTYAYS
                     KLPNVVTSMEFERLLSASGPTMGHLVRPSDHREPRKVAWLQCVGSRDIHHCDHPYCSA
                     VCCMYAIKEAVIAKEHSGGNLDTTIFFMDMRTHGKDFERYFNRAKEEHGVRFQRSRVH
                     SVEPVPGTDDLEITYATEQGSMKRETFDMVVLSVGLETTQPIRELADQLGIALNTNRF
                     SETTSFDPVVSSRPGVFVCGAFQSPKDIPQSVVEASAAASAAGAFLASARGTLVREKE
                     TPPQIDVVGERPRVGVFVCHCGINISGVVDVPAVRDYAATLPYVEYVADNLYTCSQDT
                     QVVLKKVIREHNLNRIVVAACTPRTHEPLFQETMAEAGLNKYLFEMANIRNQDSWVHA
                     SNPEMATEKAKDLVRMAVAKVALLEPLQEPELSVTQSALVIGGGVAGMAAAKAIADQG
                     YPVHLVEKTDRLGGQALSLYKTWKGELIQPYVKDLAAAMTEHPRITVHLSADITQVEG
                     FVGNFRTTVESASGSEVIEHGIAVVAKGGKGWTPNEYLYGQDPRVLVHQDLDRRFIAR
                     DPGLKEVQSAVFIQCVGSREPGRPHCSRVCCSHSVESALELKKINPDCDIYILYRDLR
                     TYGERELLYQEARRSGIVFVRYSLENKPRVEVADGKLRVTVIDHVLQRPVVFTPDLVT
                     LANAIVSDDNEVLGQYFKVPVNDEGFFVEAHAKLRPVDFATDGVFVCGLAHYPKPIDE
                     SIAQAQAAASRACTILAKKTIQFSGTVAGTNQFFCSSCGTCVSICPYSAPQFNAKGKA
                     EINAALCKGCGLCVASCRSGAIRLAGFDDAQIFRVIDSI"
BASE COUNT        11492 a        18095 c        16652 g        11902 t
ORIGIN      
        1 acggggatct ccttccccgg aggttccttt cagatgtttc atgaatctat ccatgggatt
       61 tgactcttca tgccggattt tcttcgccat ggcaggttct ccccgccgtc ggggcatcca
      121 cgaggactcc cttccgcaat cacccggaca ggcgtcgttt tgagtcccgg agggtcgggc
      181 ggagtccgct tcctatttcg aagtatagta cacccgtcaa agcttgcaaa gatgcggata
      241 aatttcccca acgggtggat gcgggggtat tgggatgttt atgtttaagg agaactcggc
      301 cgatatttga tgtcgggcgg gtgtcaatag cagaaaatgc cattgccaaa tgggataagg
      361 atcggtaatt aaagacacga actcatcgaa aaataaccgc caaggagtca caatatgaag
      421 gctcaaccgg attcatcctc agggaacctg tggtttcgtg tcggctggct gattcttttt
      481 gtctgcattt gggcaggtgt cccgaaagcc gggttcggaa gcgacgtcgc ccgggcagag
      541 gtcgaagtcc aagagaagga accctttgaa gatcggcggg tggtggtgcc cgatccactg
      601 gaatcctgga accgtgccgt tttttccttc aacgacagat tctacttttg gtttgccaag
      661 ccggtagccc aggtatacgg cgccgttctg cccgaaggtc ttcggatctg tgttcggaac
      721 agctttgaca atctcgccat gccggtgcgc tttgtgaaca acgtgttgca gggaaaactc
      781 cagccggcag gggttgagat cggcaggttt gtcatcaatt ccaccatcgg gttcgggggt
      841 ttctttgaaa tcgccgcccg cgacttcgat cttcagccct acgatgaaga cagcgggcag
      901 accctgggct tttacgggat gcccggagtg ttctacatca actggccgtt tctcgggcct
      961 tccaccttcc gcgacagcct gggtcgggcc ggggactatt tcctgaatcc ccttcattac
     1021 ctttcgcccg atatctactc gagtgccggg ttgagggtgg gcaaagagtt caacgaaatc
     1081 tctctgcgga tcggccaata cgaagacttc aagaagtcgg ccgtggatcc ttatgtttcc
     1141 atgcgggatg cctacatcca gcatcgagcc ggagaaattc gaagataaac cgccgcttac
     1201 cggtaatggg ccagaatggc ttccgtcagg gccgcaatgg tgtactcccc gggcatgatg
     1261 ttggtccgga gaccatactc ccgggcggtt tctgccgtga tgggaccgat gcaggcaatg
     1321 tccacctctt tcatccaggg gaggacctcc tcccttgggc atagctgaaa gaagttggaa
     1381 accgtcgagg aagaagtgaa ggtcacacaa tggatgcgcc gggcacgaaa cgcatccaac
     1441 acctgggtcc tgcggtccga ggggagcacg gtttcatagg caggcactac gttcacccgg
     1501 gcaccttccc gtcggagggt ttccgggagc acatcccggg ccaccaaggc ccgtggaatc
     1561 aagaagcttt tccccttcac gcccacggaa gacaagcctt ccaggatcga ttccgcccga
     1621 tattcccggg gcaccaggtc gggcctcagc ccgttcgccc gtaaggcatc cgccgttcgg
     1681 ggaccgatgg ccgccagccg tgcctttccc agggcacggg catccaggcc tgccgtccaa
     1741 agcctctcca tgaaatagtg aaccccgttg acactggtga acatcaccca gtcatattcc
     1801 gccagtgtcc cgatggcctg atccaggggt tcccaggagg ccgggggccg gatgtcgatg
     1861 gtgggaaact cgatgcagca ggcccccaac tcctcgagtc tcgtcttgaa gtcgctggcc
     1921 tgctcccggg ccctcgtcac cagaacggtt cgaccaaaga ggggacgccg ctcgaaccag
     1981 ttcaattcct ccctcagtcg caccacctcc cccacaatga ggatggcggg cggtttcagt
     2041 tgggcttgac gaaccttctc gacgatgtcg tccagggttc ccgtaacggt ttgctgctcg
     2101 gggagagttc cccagcgaat ggcggcaacc ggcgtcgtag acggtctccc gttttcaacc
     2161 aggcgtcggc agatctccgg caggttcttc acccccatga aaaacacgag ggtccccacc
     2221 gccgtggaga gcttggccca ttcaatcttg gaatcttcct tatcatctct ttcatggccg
     2281 gtaacaaaag ccatgctcgc cgtgtagtct cgatgggaga gcggaatgcc cgcgtaggcg
     2341 ggaacggcca cggccgccgt gacccccggc accacctcga agggaatgcc cgcacccgcc
     2401 agcatttgag cttcctcccc tccccgtccg aagacgaaag ggtctccccc cttgagtcgc
     2461 accaccaggt tctcccggcc cttttccacc aggaggcggt tgagatcctc ctggctgacg
     2521 gtgtggtctc cgcccttttt gcccacataa atcctttgag cggtgggagg agcccaccga
     2581 agcagttcct cgttggccag gtaatcgtag accaccacgt ccgcccgaga caggacctcg
     2641 cggcccttca acgtcaacag accgggatcc cccgggcctg cacccaccag atataccttt
     2701 cccccggaca cttggctccc tctcaaggac cggcactccc cggtccgctc cccggacctc
     2761 cgtctacggc aaggcgtgga tccggcggtt gatgttttcc ctcatgaatc gatttcaccc
     2821 atggaatgaa caaaggcggg attctttctc ggcgaagcga ccttccccca gctgcccaca
     2881 cgccgcggcc ggatgcaggg atgatccttt tgcccgcatg agccgacggg gtctcctccc
     2941 cgccgattag cccgggttcc ggtagatctc atcgagaata cgccgagccc ccgcctccag
     3001 cagttccccg gccaggcgcc gacccagggc ttccgcttct tcaatcgaag cctcggcgga
     3061 gatcacgtgg acttccattc cgtccaggga agccaccaac cccgagagcc gtattcttcc
     3121 gttctcgatc cgggcatgcc ctccaatggg cacctggcat cccccttcca gttccttgag
     3181 aagactgcgc tccgcccgga tcgccaggga cgtggcctcg tcgtgaaggg gccgaatcct
     3241 tgccgccact cgaggatcgt cctttcggac ctcgatgccg atggctccct gccccactgc
     3301 gggaatgaac cgcgagggat ccaggtacag ggtaatttga tcttcccagc ccatgcggtg
     3361 gagtcccgcc gcagcgagga ccacggcatc gtatcgaccc tcccggagct ttcgaagccg
     3421 tgtatcgagg tttcctcgaa gattgaggac ctcgatatcc ggccgttccc gacgcagttg
     3481 agccccccgg cgaaggctgc tggtgcccac ggccgccccg ggggggaggt cctccagggt
     3541 cttccctttg cccaggacca atacgtcccg aggatcttcc cgtcgcggga ccgccccgat
     3601 ctcgagcccc tcgggcaaaa ccgacggcac atccttgatg ctgtgtacgg ccaggtccac
     3661 ctccccggcc agaagagcat cctcgatctc cttcacaaac aggcccttgc cgccgacctt
     3721 ggccagggga acatccagga tcttgtcccc cgtggtcttg atgatccgca attcaacctg
     3781 cagatccggc cataagctct ccaggtccct tttgacgttt tcgctctgtc tcaaggccag
     3841 gaggcttccc cgagtgccaa ttctcagtct cgtgcgggcc aatcaaacct ctctttcgct
     3901 tcaaccccct cgggctaatg acaggagctg cagctgccgg aggtgcatcc cgcacaaccg
     3961 gagccgcccg atcccatgcg gctgctcgca gcccccttgt ctcctccgct cttgaacccg
     4021 aataccgaca acaggcgctg aacctcggtc gattcgcact tgggacaggt cacggcttca
     4081 tcgctgccaa agacaagccg ttcaaacctc tcgcagcaac gacagcactc atactcaaaa
     4141 ataggcatac tcttttcctc caacgaaaat cctacgggac ccccggtcat cggtgcgccc
     4201 cattgtaacc catcccgtcg gggatggcga aggtcccacg cgatggttcc gggaactttc
     4261 cttcgtcttg ggtttactct ccgtcgattt ccaccgggtc tccctcctgc acctctttga
     4321 aggccgcagg atctcccagg actttcccta tcagattgac agcgctggcc ggtcggattt
     4381 cctctccgcg actgatgggg gtcggtccga agaagatgca gaaggccttc cctgcgggcc
     4441 aatagcccag gtccccgact tccaccgttt ccacggcggt ttggtccaac gggcacgaga
     4501 ccggaatggg aaagtagatc tcatcgcccc acgtccgggc cacggccttc aggggcaaag
     4561 cggccccaat ggcgcaagcc gtaggcgagt cgttgagctc ccccgtcaac cggacggagc
     4621 ctgcccggat tccaatgcat ttacccatga tccccccttg agctcaaccg ctccgccacg
     4681 gctgccatga ggagcggaaa catgatttca tggtgcccga tgagaaccag ccccctgccg
     4741 ctcccgcagg tgggacgatc gaccacgttc acctgggttc ggtagtgttt ctggaaatcc
     4801 atgttgatgg tggtgagccc gtcaaggtcg aagcccaggt tccgggcaac gctgaccgcc
     4861 ttgagaaaca cttcggggag gatcaccgcc gagcccaggt tcaggtagac cccttctttg
     4921 agagcactca ccagcctgca gaacacccgg aagtcgtagt ggctgagggc tccggtggcg
     4981 gcaccgtcca tggcggcatg catgtgaacg atgtccgttc ccacggccac gtgcaccgtc
     5041 acgggaattc caagcctgga cgccgaggca aggagactcg aacgggcgaa aggcgcccga
     5101 ttttggagga gaaattcccc cacggcggcc cccaggccga tcccgccgcg tcgatgaccg
     5161 gccgcgacgg cagcgtggat cagctcgctc gtctcgcgaa ccgcaccgaa gagcccccgg
     5221 tccaagtggg ccgaaacatc ctcggaggtt tttcccgcga gggccacctc cacgtcatga
     5281 accacgcagg cgccgttcat ggccagtccc cggaagagtc ccttctccat ggcatcgatc
     5341 agcacgggat tgagacccac cttgatgggg tgcgctccca tccccagcaa gacggtcctc
     5401 cggtttcgaa cggccgtggc cacggcatcc gcggcccggc gcaaatcccc ggcagcaaga
     5461 atgtggggaa ggccttccag aaaatcgcaa agggacccgc ccacattcca gggtttgccg
     5521 aaatcttcca tgcacacctt gctgggtcgg tcggccaggt tggtggtgcg aaggccgtcc
     5581 agaggaagcg gttccacgga ggcgttcttc ttttcgggag tcacggccgc cctccgtaaa
     5641 gctcatcctc gaccagttcg cacaggatgt ggcccacggt gatgtgacat tcctggatcc
     5701 gtgcggccac cgtggaaggc acgtgaagga cgtggcgaca atgacgatcc atggggcagg
     5761 gagcgactcc cgccagcccc aaagtacaca ggtcccggtc ttcggcccat tcgagagcct
     5821 cgaggacatt gggggaagtt ccgctggtgc tgattcccag agctacgtcc ccggcccggg
     5881 cgaaggcctg cagttgccgc acaaatacct tttcgaagtg tagatcgttg ctgatgctcg
     5941 tcagcaccga ggtatccacg gtgagagcca tggccggcaa ggcgggccgt tctttctgaa
     6001 aacggttcac aaactcggcg gcgatatgct gggcgtcggc cgcgctcccc ccgttgccga
     6061 aaagaaacag tctccgcccc cttctgaagg ccagggatag gacttccgcc atcctcaaaa
     6121 tggtccccgt ttctttcccc agcagttcct ctttgagtcg gatactctcc cgaaaggcct
     6181 ctgcgacggt tgtttccatt cggcttgtgc aaatctcatc cattgggaac tctctctcgc
     6241 ccccccgaac gccggatcgg gacggatcac cctattcctc cggaacctat ggcgcccaag
     6301 ccaccccgag cgctttaagt ggttgctttt gccggaataa cggaagaaga ccttgtccac
     6361 gaggtgcact gcgactaata ttagggcggc tatgggtttg gaaaaagcgc agacacaata
     6421 gtcaagagcc catggatctg caagggaaaa agggacccat ttctccgtga tgaactcagc
     6481 tcggcccaac aacggtaacg ggaaaattcg gagcatcgtg ttcgactttg acggcacgct
     6541 cgcggagttg ctgctggatt tcgccctcat gaagtccaac ctgggaaccc tagtggaaaa
     6601 gtatcttccc ggccccgtcg cgcccccttc ccttccggcc ctggaatggc tcgaagagct
     6661 tgccacggcc attgggcacc gagacggaac cgcggccgaa gacttccgcc gcgaggccca
     6721 tcgcatgatc tcggaaatgg agatggaagc ggcacatcac ggaaggcttt tttccttcac
     6781 ccgtcctctg ctgacggaac tgagacagct gggactttcg gcggccatca tcactcgtaa
     6841 ctgcgaacga gcggttcgcc tggtcttccc cgacctcgag gattactgcc gggcgttttt
     6901 ggccagagac catgttcctc tcgtaaagcc ccatccggac catcttctga gggctttgga
     6961 tgccatggga agcgactcgg caacatccat gatggtaggg gaccatcccc tggacatccg
     7021 gacggggaag aacgccggaa tcctgacggc aggcgtggca acggggaaaa caacccccgg
     7081 ggaactgtca cgaagcggcg cccactggac tgcgaacaat tgcatggatc ttttccggga
     7141 actgaaggcc gccggttgga tttgagggtt cccgcaaccc tgcgtgaaag cgaattccct
     7201 tatggaccta gaaatattcc aaggccccat ccggacccgg cgagccatac tcagcttcac
     7261 cggctggccg gatgccggag aaaccatcca gcacaccctc aaggaactcg agcggggctt
     7321 cccctgcgaa ctggtcgccc gcctggacat ggacgggttc tggcataccg aatcctcccg
     7381 cccccaggtc tctatccagc acggcaggat ccagaacctc gagtggccct cctactactt
     7441 ttttctcagc aattttgcct cttcggaacc ggtcctcctg gggaccgggc cggaaccggg
     7501 gtttcgatgg cgggccttta cccgtgagtt cctgcagctc ctcaccgact ggggatgccg
     7561 acagatcttc ctgttgggga gccttttcga ccagatcttc cacgacgaga tcctcctttc
     7621 gagtgtcgtc caagacccgg ccggtttcaa cctggtgcgg gaactgggat gtcagcgcgt
     7681 ggaatacaag ggacccgccg ccatccagtc ctccatcatg gagaacgccc atgaggccgg
     7741 tatccagtgc atttccatct gggctcacat ccccttttac ctgagcggcc ctcaccaacc
     7801 cgccatggcc cagtgcctgc aaattctggg aaggctcctg ggactcgaga tcgacacccg
     7861 gccggccctc caagggtggg aggagaaggt gcaggaaatc gagaccctca tccaacagga
     7921 tgcggacctg cgccaaatcc tcgacggcat gcgaaagaac ccggattccc gaaaaccaag
     7981 gtccgtactg gtaccgtcca aggtcatcca gttggacgag ttcctcagac gccgtcagcc
     8041 gacctcggag cccgagtgaa ggttttcacc cttacccaag aggaggattc atggtccgtt
     8101 ccggactatc ccctgaaatg accgctttcc tggactacac cgtctcccac ttcgaggaaa
     8161 tcgcccgtcg gaatcgtttc cccgaaaaca accaggtgga acatgaggaa gggcgctgcg
     8221 tggtgtgccg cccgcacctg gtcgccatgg aacccttcgc tgcgtacctg gaggtggtag
     8281 cccggtccgt gatcgtgagg cgtccgaccc tggaccaagg cctggtggac gaaatcaacc
     8341 acgactcgat ccttgcgggg ctgcccctgc gggtttccgt ggaatccatc cttgcggggg
     8401 agggggaatc tctccggggc tggagtcgct gggttcgaga agcattggat acgggattgg
     8461 ggcttctgtc catccacagc cccaccagcc gggaattctc tctagaggaa gccgaacaaa
     8521 agggatttgg ggggttgatc gaggagatgg tgcgggaaat catggctttg caaaagagga
     8581 accggacggg agattcccgg tccctcgcgg gatgatcgcc ccgcacttcc ggggtgggcc
     8641 cgcaaaaaga gccgggtgaa gcccggctct tgaagtttcc gtagtctcga tctgcgcggt
     8701 gtcttaaggg ttacagtttt atcactttta cggcctgggg ccccttggga ccgtgctcca
     8761 cctcaaagtt gactcggtcg ccttcactca aagaacgaaa tccatcggca acgattgcag
     8821 tgtgatgtac gaaaacgtcc ccctgatcct tggtttcaat gaaaccgtac cccttcttgt
     8881 cgttgaacca tttgactcgc ccttccggca ttgtaattcc tttccttctt tctgtcccct
     8941 actcgttgcc ggtgcgtacc tttgagagca tcctcgcatt cgcctggtat acagggaaac
     9001 ctgccgacta aagctccaaa acaccgaaca cccaaccctc acatgttact tgtgtagcgc
     9061 atgggagagt cggtgtcaag aaaagttccc aagagaagta ttagtttggc aaaatttgtt
     9121 aatgttcatc aaaatcgaaa agtttcgtct ctttcagatc ttctcgtgtc attgagcgga
     9181 gttagagaaa aaccgtctgg cattctctcc ccctaaaacg ggcaggatga ccgcaagaaa
     9241 agggttcccg agggggcatt ccccccgtcg gcatcttcac gggacgggac gatctcgcgg
     9301 ccccgtgctc tttcaagaaa gcagatcccg gacagcctct tcgaacaggg ggaactcgaa
     9361 ccgaaatcct tcccggagca accgttgggg gtgggctctt tgtcccttga gaagcaggtc
     9421 tccgaactcc ccgagcacga gccgcagggc aacggcaggg gcgggcggca ggaggatcgg
     9481 tttcctcaag acccgggcca gggtccgcgt cagttccgca ttgcgcaccg ggttgggagc
     9541 cacgcaattc accggaccgg agatgtccgg cctgccggca agaaacgtca tggctcgata
     9601 gaggtcttcc aggtggatcc aggggaacca ctgttttccg ctgcccaggc ggcttcccag
     9661 gaagtactga aacatggatg ccatttgttt tagggcaccg cccccccgcc ccatcacaat
     9721 cccaaagcga cagaggacca cccggacccc caggtgctcg gctctcccgg cttcagcctc
     9781 ccaggccatt gccacccgtg ccaaaaagtc cttgccggga gggctgtctt ccgtaaggag
     9841 ggtatcgtcc aggcgccctc cgtaataccc caccgcggag gcactcagaa gggtggtctt
     9901 cccttcggcc ccggacaggg cgtccaccag attccgagtg gtccggatcc ggctttccag
     9961 gacggcattc ttggcctttt ccgtccaata ggtgaagatg gaagcccccg ccaggttgat
    10021 gagaagatcg ttggccggga ccgcttcctg ccaggcaccc gcctgggtgg aatctccctg
    10081 cacgtaatgg acccgcccga gttcgccgga ttccttcccg ggggaacggg acagcaccga
    10141 cacctcgtgg ccctcttgga ccagcctctt gacaagatag gtacccacaa acccggtacc
    10201 gccggtaatg aacactcgca tgacatcgcc tccctgtccg cagcccgcag aaacgatccg
    10261 agctcgtggt ggtcccgttt agctcgaggg ccttccctct tctttgccct cctcccggag
    10321 gggatgcccc gtcccggggg ccgtcgagtc caaggctgca agggcctccc gggctaaatc
    10381 cgagactggc ccggaccaaa gtttgccgtg gagatacgtt cggatctccg aagaatccgt
    10441 cgtcagggga tcgaggctcc ggcgggcttc cccggcgccc agggccccca gaatccacgc
    10501 cccgagaccc cggtggaacg ggtccgggga atcgaggtag ggaataaagc aggaaaaaca
    10561 atcccggagg agttccggct tggcgtgggc caccctcccg agaccccaga gggctcccct
    10621 ctcgagaaga gggttctcca ggcgattccc gtcctcccgc acgtaggaca gcagaatgca
    10681 caggtactcg gccgccaggg cggcgtgtac ggccatcgtt tcacccatgg cttcggggat
    10741 gccccagccg atccctcccg attcgtcgtt gaggttccac atgaatcgcc gcaacatgtc
    10801 ccgggcggat tccatgtccc ggtccgcgag cttcctcatc tggaacccga cggcctccac
    10861 gctcttccat ttcaagaact cgtcgccgct gcacaggaag gcaagaaggg cgttgaggac
    10921 ggccttgggg ggagcatcga gggcttcgag ctccctttcg aggttttcag atcggaggca
    10981 ggcctcgatt ttggccttga gggttcccag gcgcgatggc ctcgcgggaa gtaccaaaga
    11041 ggacatgatc aatcctcctc ccaccggatg caccccgaag gacagcaatt gatgacttcc
    11101 caaatacaat cgtcctcttg gggtttttct tccttcacct cgatgtagcc caaggggctc
    11161 cgtcggaaga cccgggggca cagttccacg cacgcatcac agccgacgca ttctcccata
    11221 tccaccgtca gcgatttgct catgaggaat tccgcttcct tcttcgctgt gcaattcgtt
    11281 ccgtcccccg gaggaaaacc cgatctcttt caaccgaaat cccgtctcct ccgccggtcg
    11341 gcgatttcgc ctttcatggg ctccgcgttt gccggacttg cttgcgcctc agcgttcctg
    11401 ttgagggccg acgtagaacc ccttttcttt cgccgagaga aaccccggtt cctggcccaa
    11461 gataggaacg gggcgggacc gggtaaaggg agagtcggat ccgaattctt gacaaaatgg
    11521 gattgacccc ggaaacacaa ctttgttata ttcctattca tcaatgtact gacggggtcg
    11581 gagcccaagg tttgaagacg ttccattcgc ggtttgaggg aggaatccat cgtggctaac
    11641 gttcaaacgg tcgaaaagga tttgacaaga gaggacacgg cggaaagctt tgaggaaatg
    11701 cagcccggcc tcatcgagtg gaaatccatt tggatgccca tgcttttcgt ggtggggatg
    11761 ttcctggcgg cattctatct gcccgtggag agcaagcgct ttaccaacgc cgttctggag
    11821 tccctggccc tggtcaagtg gtacgcccgg gaacatgtgc tcctttgcct ggtgcccgcg
    11881 ttcttcatcg ccggggccat ctcctgtttc gtatcccagg ctgccgtgat gcgctacctg
    11941 ggacccacgg ccaacaaggc ggttgcctac ggggtttctt cggtttcggg gacgatcctg
    12001 gccgtctgtt cctgcacggt gctgcccatc tttgccggca tctggaagcg gggagccgga
    12061 ctggggcctg ccattgcctt tctatactcg ggaccggccg tcaacatcct ggcgatcgtc
    12121 ctcacggcga ggattctcgg gctgcccctg ggcatcgccc gtgcagtcgg ggcggttctt
    12181 ttcagcgttc tcatcggatt gctgatgcat tatttcttcc gccgggagga agagcaaaag
    12241 gtgcaggcgg cgatccacat gcctcccgtg gaagtcgagc ggcccttgtg gcagaacgtg
    12301 gtgtacttcg gcaccatggt gggtctgctg atttgcgcca cctggggcaa gccttcggag
    12361 ccggtaggtt tctggaacgc cgtctacgaa atcaagtggt ggctgacggg cgcttttgcc
    12421 gttgccctgg gaatcgagct ccacgtctgg ttccgggtga aaaccgccaa ggttgtggct
    12481 gcagccgttg tcgtggccgc tttggccttt tcttttcccc aagagcccct catcgctttc
    12541 gcggcgggca tcgtcgccct caccgtgctc atcaccacaa cccgggggga ggcccaagaa
    12601 tggtttctgt ccacctgggg ctttacccgg cagatcaccc ccctgttgat cggcggcatc
    12661 ctggcggccg gactcctgct ggggcaaccc ggcacggagg gaatcatccc ctcgcaatgg
    12721 gtgagcaacc tggtgggggg caattccctg ggggccaatt tcttttcggc ggtggtgggc
    12781 gccttcatgt atttcgccac cctgacggaa gtccccattc tgcagggcct cctcggctcg
    12841 ggaatgggca atggaccggc cctggcactg ctgttggccg gcccggcact ttccctcccc
    12901 aacatgctgg tcatccggag cgtcatggga accaagaaga cagtggtcta cgtgagcctc
    12961 gtggtcgtta tgtccaccct ttgcggcttg atcttcggcc actttttccc aaacgagccg
    13021 tagttcgttc cacggtttcg ggcttcgcgc ccaacacccc atttggaaag gagataggta
    13081 tggaaatcaa agtcctgggt cccggctgtc ccaaatgcca ccagaccgaa aaggtcgtga
    13141 aggaagccgt tgcggagagc ggtgtggacg ccaagatcga taaggtcacg aacatccttg
    13201 agatcggcga gtacggtgtg ttcggaactc cggccgtggt ggtggacggc gaggtcaaat
    13261 cggtgggaaa gatccccacc aaggccgaag tgctcaaatg gatccagaag taatccatct
    13321 cgaaagggga ggaacgagcg atgactcagg aatgctgcac accgggcgga aacctcatga
    13381 tcctcacgtg ttcggggggc tccaatgtcg ggcagctttc caaccaggcc gcagtggaac
    13441 tgacccggga aggcttcggg aagatgttct gcctggccgg gattggaggg cgattaaaag
    13501 gtttcgtcca gtcggccaag gacgttcccc gcatggtggt cgtggacggg tgttccctgg
    13561 cgtgcgccaa ggcctgcctc gaccaggcac aggtacccgt gaagaactac gtggtcctga
    13621 cggacctggg cattgaaaag aacaaggact tcgacctcaa gcgggaagag atcgaccggg
    13681 tgaagcaggc cgtcaaagcc gcctgcgcaa cttcggacgg gtagcggaca acctctcggt
    13741 ccgataaggc gacggggaat gccggtacgg ttgttccccg aaaccttcga cggcattccg
    13801 gcgaaaaacg aagtccggat ttcataaggt tcaccgtacc ccggccttcg ccgtggagac
    13861 gtccggccaa ggacccgggg ggggtccggg gatcgggtgt gcaagagggg gtatcccatg
    13921 ttgcttcaag ggtatcgcaa ggaaattgtc cggccggaat gcaggcggga ggccgaatcc
    13981 ctccactgca tcgcccacct cgaccaggac atcggggagg tgctgcccta tctcaacacg
    14041 gtcttgggcg gttttcaata cacccgggac cccccttcgg tatcgttcaa ggcccacgga
    14101 aagctcatcg ccgtgcatcc tcgaatgatc tgcgtcaatg cgctcaagga cgaaaccgaa
    14161 gggaacagga tcctggagtg gctcaaaggg gaaatcaacg acgcgtggga gcggcgggcg
    14221 gagatccggc ccagctatga aggggctccc aaacccaggc tgctcgaaat tctcaggctt
    14281 cttcccaaga ccaactgccg ggagtgcggc cagcccacgt gcacggtctt tgccgcacag
    14341 gtggccgaag gaggcaaggg ccccgagagc tgtccggcca ttagcccgga aaacagagaa
    14401 agactcgaag cgtacctggg tcggttccga ttcgaatgac cgcaaaccct ctagaccggg
    14461 gagatttgcc tgtcatggcc ggagagcacg gaaaactcaa aattctcttt ttgtgtacgg
    14521 gcaactcctg ccgcagccaa atggctgaag gctgggcccg gcacctgaaa aacgaccgca
    14581 tcgaagccta ctcgggcggg gtcgaccctc acccggtgga cccccgggcc gttcgggcca
    14641 tgagcgaggc cggggtcgac atttcatccc aggtctccaa ggatgtggat accttcgagt
    14701 ccatcgaatt cgactacgtg atcacccttt gcgaaaaggc caatcggtcc tgccccgtct
    14761 tccccgccaa gacccgcttg gtgcacgttg ccttcgacga ccctccaagg cttgccgcgg
    14821 aagctcattc ggaagaggaa gccatggccc cctatcggcg ggtgcgggac gagatccggg
    14881 aatttgtgga aaaactcccc gagatactga cggaagagga ccggtaggga gaaggagcag
    14941 tgccatggag gatccgggac ccaagaagct ttcctttctg gaccgttttc tgaccgtgtg
    15001 gattgtatcg gcaatgctgg tgggggtggg ctggggatac ttcttcccgg gcgtgcgggg
    15061 aatcatcaat ctctttcagg tgggaacaac caacattccc atcgccgtcg gcctcatcct
    15121 gatgatgtat cctcccctgg cacgggtgag atacgaggaa ttgcaccggg tcttccgcaa
    15181 tcgcaaggtc ctgggccttt ccctcgtcat gaactggatc gtcggtccca tcctcatgtt
    15241 cgtcctggcc gtgatctttc ttgccgatta tcccgcctac gccgtgggcc tgatcctggt
    15301 gggcctcgct cggtgcatcg ccatggtcat cgtctggaat gagctggcct gcggcaaccg
    15361 agaatactgt gccgggctgg tcgccttcaa cgccatcttc cagatgctcc tctacgccgt
    15421 ctatgcctat tttttcatcg cagtgcttcc caagtggctg ggcctggggt ttgccacgga
    15481 aatccaggtt tccattccgg acgtggtgaa aacggtcatg atttacctag ggattccctt
    15541 cttcggcgga ctggctaccc ggctcatcct cataccggcc aagggaaagg agtggtacga
    15601 aacccaattc atccccacaa tcagcccgat caccctcatt gccctccttt ttaccatcgt
    15661 ggtgatgttc tccctcaagg gcgaatacat cgtcaccctg cccctggacg tggtccgggt
    15721 ggccgttccc ctggccattt atttcgtcgt catgttcttc ctgaccttct atctctctca
    15781 caggctccgg gcggactatc ccacctccgc gagtctctcc ttcacctcgg cctccaacga
    15841 ctttgaactg gccatcgccg tggccatcgc ggtgttcggg ctcgaatcgg gccaagcgtt
    15901 cgccacggtc atcgggccgc ttctcgaagt gcccattctc atcagcctgg tgaacgttgc
    15961 cctgcggttc aagcagaagt attttgccga agatacctgc acggtttgcg gagtggcggc
    16021 cacgaacacg gtcctcgttc agtgccgcca aggggagaag gaggtccggg tgtgtacgga
    16081 ctgtgcccgt ggttccaccc cctcagggaa ttgagaagga agggcttctt gtttcgaacc
    16141 cgctcctagg gagcatgaaa cacctggacc tgcacctcct gctttccttc ggaggccatg
    16201 tccgtttcga tggaaagaac cgtccgctgg ggtgcccccg gagggacgtt ctccatccgg
    16261 ggaacgatct cgaggctaac gccctgctga tccggaaggg gactctgggt gacccggatc
    16321 agcttctcgt cgtggaccag gttgatgatt ttgaaaggct ttccgctgtt ggacgccacc
    16381 acaaccctcc ccttgcgcac cggctgctga gccgacagct ttccaacgag cagggccccg
    16441 ggcttgacgg aaatctcccc ttcgatggaa ccgttgacgc ggatgaggat ttcgcttttt
    16501 cgttcaaaat ccgtgtgcag ccggaggaag ccgctgtaat ggccctgccg ggcaagattc
    16561 gtcacctgca accggtagag cttcccgtcc tccaccgtca ccagttgatg agcgattttg
    16621 tcctcgaggt tggactcgac ccgggtgatg cgaaagggtt gcagggccgc tgccagatcg
    16681 agggtgcttt ctcccatgga acgggccgga ccccggaagg cgatcgtatc cgccgggcga
    16741 acgtcgatga gggttcgcac cttcccctgc atggtcaagg ccgtccgggg gtttcgggga
    16801 tcgttggatt cgatggtggc gtttttttta atatttcctt tatagccctt gaggatcacc
    16861 ttcagcgtta ccgttccctc gcctccggga gggatggccc gatcaaagcg ggtgaccacg
    16921 cagcctcaat cccggctgac cttggtgatc cgcagttccc cctttccccg gttcatgacg
    16981 atgaagtcgt gggagatctc actgccttca tccacctcgc cgaactcaaa cttcgtctcg
    17041 gccacggtga ttcggggggc ctcaccggga tttccggggg aaaaggagct ttcttgagaa
    17101 aaggcgggga atgtgccgga aggaagcccc cagagcaatc ccgacagtac cgctatgaaa
    17161 ccgcgaaacg ggagtgatct tgccacgact tggttattcc tttccgaggc ccggcgaagg
    17221 agccggcctt cattcaacac gcgaggtaca cggcctcatc gatccgcact cgtaacacag
    17281 gaagctctac gaaacatccg aaaaaggcct caccacgaaa tagaggccca ggattcctac
    17341 ggccgtgccg gcgagtttcc tgaaccattg actgctttca tgaaaggaac cgctctcgag
    17401 gagcctcctc accttggccg tcgaactccc ggcgatggcg atgggaatgc aatgcccaat
    17461 accgaaaagg acgatgtagg caattccgat gaggatcttt tgctgaatgg taataatggc
    17521 taggatggga gcaataaagc cgaacgtgca ggagccggaa aggatcccat aggccagacc
    17581 caggacaaag gcgcccgtaa tccccttgat cttgatgcga cccagaaggc ttcccgacat
    17641 cgagcatgcg gaaatccccc acatatccag agccacccag atgaggactg ctcccaccag
    17701 gatggtccag taaggaccga tctcccccag catgcggccc agtagcgagc atatgattcc
    17761 caccagggca atcgagataa aaaggccgac actgaatacg atagcatacc aagcagcatc
    17821 tttcgcctta agggctttat cctgaccggc cacataccct acaatgagtg ggatggacgc
    17881 aaggtggcag ggactcaacg ccacgctgac cattccccaa aggagacagc caagagcggc
    17941 gatcagcaca ccccccatca tccagtcgtt gattgttagg aagaactgat ccagcatttt
    18001 gcccctcctc acagaaccat tgtttgcagg cggaccaact ctttcatttt gaccccgtct
    18061 caacccgaat atgcctcaac acactctccc atgcgtcgcc aaagaatcgg agaaaccatg
    18121 aagagtccat ttcccaaaac ggtgcgagcc ggttctcgga atcccggttc actctactct
    18181 cagctttcgc aaggtcgcca cgatgtcctt cttttgcatg aagccttcgt gacgttgaac
    18241 ctcgttgccg tccttgtcat agaagatctg cgtgggaatc gaccggatcc cgaatctgcg
    18301 accttgttct cggttcttcc agacatcaat gaagtagatg gctgctttcc ccgtgtggtg
    18361 gtattcgctt tcgagctctt ccatgatggg ggccatcatc ttgcatggaa tgcattcgtt
    18421 ggccccaata tccaccatgg tgaccttacc tttggccggg atctcaggga tctccccggc
    18481 cgatttctcg gcactgcacg atagggtgaa cagccccagc agaatgagga gaaacatgag
    18541 acccgttgtt gtctgaaatc gcagatttga tttcatatag actccttcca aatgcttcgt
    18601 gttgatcgga cccataactc cgacattgaa agcagcgtct ccgcatcttt tcggacgcga
    18661 cgccctttcc cacatcctgc tcaccgaaga gctaccttcc tcaccaccga atgcacgggg
    18721 atcacgtccc cgggtcgggg ttgataactc aagtccacgt ggacactcac ttctcccccc
    18781 tccgccggaa ccgggatttc gaaaacctct tcccgggaac cgaagggctg gatggacgta
    18841 tcccgaatga ggcccagctt tgtgtccggg ccgaggacca tggtcgaacc cagaccgtcc
    18901 gtggattggg gcatgtagat cttccgggcc tcaaagaatg cttcctgggt ttgggcgtgg
    18961 gcggccacat acaggaccac tcgattcttg gacggtcacc cgtcgggaac ccggtgcccg
    19021 gccttgctcg tgatcctggt cctgactacg atcatggggg ttttcacgtt ctccgccggg
    19081 tgaaacgtat aggccaggac ctggacctcc atggggaggg ccgctgccag gcgtgcagaa
    19141 gtttcatccc tgtcattgaa attcggtcgg accgaatgat ccttgtgggg catgtgacaa
    19201 tcctggcagg tttgagtccc gcctgcaggg atataggcgt ggaggtaact tccgtaaaga
    19261 gtggcgcact gcaccggagg aaaggtgtcc aggttcggtc cctggccatg gcactgaccg
    19321 cagaagacag gatctttcat taccggactt tgcctgaccg ttgagtattt gcccccgtga
    19381 tcccgcagct ctctcgagcc gtaaagggca tccgtctcgg gacgatgatg aacgttggcc
    19441 ttctcctgat gacagaccag acaggtgatg cccaattttc gcaccgtctc cttgtcgtct
    19501 ttcaagatgg ccgcggcaag ctcttttacc gcctcgtccg ttgcgaggct caactgggga
    19561 aggtgacatt tggcgcacgg gaaatttgcc cgagttgcct cccgggcgga tcgaacggcc
    19621 agtgcaccct ccttgagata cttggaaagg aagatccagt cgttcaaacc catgaggggt
    19681 cttgcatggt gtgagctctc ccactggcgg tagattgcct cgtgacattc tctgcaaccg
    19741 gacgaatcga acctctcgat caggccggaa agggaatgga tttcgggagg atggatcgtg
    19801 cctccatgag cgatccacgg cagaatcgcc aggacgagga agaaaaccgc tccggacctc
    19861 ccggccttcg tgaagcccct atccgccacc ttgatcctcc acccgctcaa accgccccac
    19921 cgagcgatcg gccgtgtaaa tcctccccgg ctcgtgaata tcattcacaa agccccaggg
    19981 cattcaggtt ggatacatcg agttcttccc gagatttgtt gagcgtttcg gcgacctcct
    20041 cgagggattt tccccgcgag acgcggccat cggcatattc cagagcggct gcaccgatat
    20101 actttgcctg aactcccgtc agccgagact ggagggcaag gcgtttggct tccagtatgc
    20161 tgcagcgctt aaccagcacg agatatcggg caaaaaggat ctcccagggc tcattccagt
    20221 cacgactcca ccgtgcggcg gcgtccacaa cctccccgga cgcctcgttt tcactctcct
    20281 cagctgtacc catggtctcg atgatcttct tcatatccgg ccagccgggg tgttcgtaga
    20341 gcgactttac ctcgcgacgg aggtccagcg attctcggaa cagctgatcc gtttcatccg
    20401 caaccacagc cagggccgca ttggcctcga tgattcttgc ctccgactgc gggcttttct
    20461 ggggaatcgt cacgcagccg ccgagagtcc cgacggcaag aaaaagcaac acaaaagctc
    20521 gaaacatgga atggatctcc ccctgggacg aaggttagga acccgaaagc cgaacgatcc
    20581 gaggatacgg catccccgaa aacaaaaaat cccgtgcaca attcatagac tccccatccg
    20641 acgaatcttg gggaggtctc ggtgcaactc gtccaacggt cctggaggga gtggacgctc
    20701 cctcgattga gcgacttggc cggtcggaat cccgtttcgt cattcccgcg ccagcgggaa
    20761 tccggaagaa gagtgcatac ccgccggacg cccgtcttcg cgggcatgac ccccccgatg
    20821 ctctcggcat cgctcaactc agggtcaaga gacctctacc ccacaatccc gggggcactc
    20881 gaaatgccct ccgcacggtt caggcaccct tcaccgcaga taccttgatg cttgcaatcg
    20941 tggaggagag ctccttcgct tcctcccggg aaagattcaa atccgcacag attggctgtg
    21001 caatcggatc ggcagccatg agctccaggg gataggtgtc ctggccgagg acccggatct
    21061 cctgaaatcc agcggcttca acggcggcga ggtattcgtc cttcatgacg gctccggcta
    21121 tgcatccgac gtacgctccc acggacttgc gaatggctgc ggggagctct ttcaacagaa
    21181 ctatgtcgga aaccatcagg cggcccccgg gcttgagcac ccggaacgcc tctcggaaga
    21241 ccttttcctt gtcgggagaa agattgatca cgcagttgga gatcaccacc tccacctcgt
    21301 tctcggaaac gggcaggttt tcgatctccc ccaggcggaa ctcgacgttg tcaaaattcc
    21361 cctcccgggc atttcgcctc gccttttcga ccatctccgg cgtcatatcc accccgatga
    21421 ccttccccgt gtttcctacc ttcgaagcgg caataaagca gtcgaagccc gccccggatc
    21481 cgaggtcaag aacggtttct ccttcctcga tggatgcgag ggccacggga ttcccacagc
    21541 cgagccccag gtttgctcct tcgggcacgg cttcgagttc ctcgtctccg tagccgactt
    21601 ttctgctgag ttcctccgcc aaattgggac ttccgcaaca ggacttgccg gttccgcagc
    21661 agggcagatt tgaaagggcc actttggcat aactcgattt cacggcattc ttgatctctt
    21721 cggctttcat ttcacgttgg ctcccctgaa agatttggac ctagtccgca ctcaggctcc
    21781 tcagcaccgg accgaggaac tcctggacac gccctgccag agcgtccgcc ttgatgagag
    21841 tgagacagca cacctctccg tccttttgcc agctgatgag tgcaagcttg tctcccgctc
    21901 ggatttttgc cctgtcccga agttccttgg gcagcaccat ctgtcccctc tcatccacac
    21961 tgatgagcga ttcgatcctg cagcaatcct tccccgtggt ttcgggatcg cagcagggtt
    22021 ccttcttgct ctttctgggc attccgacgg cctcccattg cgggtcctgt tgaatcagtt
    22081 gattccgatt gatctgattc atccggagat agtccaaacg ggaccttttg tcaatccgtg
    22141 aagccggccc cttctcgtgt ctgccgggaa ccgaccggta cgaaaccttt tggggcaacg
    22201 caggcgctga gggaccgggg tccccaattt cttgaagtgc cggcatgcgg ttcaaggata
    22261 tagtccgaat tgttcgggga ggaaaggccg tgaggacgga aaagggttcg tcgaatcttg
    22321 cgtggaagcc tcccttgaaa agggggggcc atagaacgtg acgatttctc tcttcacaga
    22381 ccaaggaagc cggggatacc cggcgatcgc atcatggatc ggaatcggat caagcccggc
    22441 tgatgagata gttgtaggcc gaaagggcgg ccttggagcc ttctcccacg gccaccacca
    22501 cctgtttgaa gggaacgttg gtgacgtccc cgcaggcaaa gacgccggcc aggcccgtgc
    22561 ggcactcgtg atccaccaga atctcccctt tttcgttggt cgtcaggata tgggaaaaga
    22621 ggccggaatt aggcagggtc cctatttcga tgaacacgcc ttccaccgcc agggtcttga
    22681 tttctccgct ctgcataccc cggtactcga tcccctccac gtagtcccgg ccgaaaattc
    22741 tcgtgggttc gcactgggga aggatcacca ccttctcgga ttggcgcacc ctcgcctggt
    22801 aaacggggtc ccccgtcagc tcgggcatca ggaagatcag gtgaattttg ttggcgatgc
    22861 ccataaggtc gatggcagct cccagggccg agttgcctcc tccgatgacc gcgacatccg
    22921 ctccggcata caggggtgcg tcacaggtgg aacaatacga gactcccttc cccacatatt
    22981 cccgttcccc ggggatgttc aacggcttgt ggcgacctcc cgtggcgatg atgatggttt
    23041 tcccccggta gctccttccg tccctcgtca ccacccgctt gacgggaccc gccggaaaga
    23101 cctccgccac atccaccccg atggctcttt ccacgttgaa cctccgaaca tgttcctcga
    23161 atttggccac caactcgagg gcgttcacct ggctgaaccc caggtagttg tccacgtcgc
    23221 tggtccaggt gagctgtcct cccacgtcct tgctgaggat gagggtcttc atggccttcc
    23281 ggatggtgta gatggccgcg ctcagccccg ccggtcccgc tcccagaatg atcacgtcgt
    23341 acagaagctc ttcggccttc tcttcgttcg tcatgggatc cggtctcctc tcagggctgg
    23401 tcgcctttga gggcttcgtc caggagctcg ggttcgaacc cgatgatgac cttctccccg
    23461 atcgagatga cggggaccga ccgtgctccc ccggaaacct ttttcatttc ctgaagggct
    23521 tgggggtcgc tgctcacgtc caccgcttcg aactcgacgc ccttttccgt caggtactcc
    23581 tttgcctgcc gacaatagtg gcaggcagcc gtcgtataga ttttcacctt ttttgccatg
    23641 aaagccccct cttctttttc cctcactgca cggtcggatt tccactcgaa cggcggggac
    23701 gcccatcccc ggtgaaacaa aggaaccggg cacggagcat cccccgggga ccagggacga
    23761 gaaaccgtcc tgatcactct ttcttgaagt cggccttgga tgcgccgcaa atggggcagg
    23821 tccagtcgtc gggaagcttt tcaaaggggg tgccgggagc gatcccactg tccggatcgc
    23881 cttctgccgg gtcatagacg tagccgcaga tttgacacac gtaacggtcc atccttcggt
    23941 ctccttttct tgtggctggt atcggggtaa gttcggagca acccgggggc tcatccccac
    24001 gatgaactag aaagtcgccc catatatatc atcctgcctg tagttggata agcattttcg
    24061 gatgaatgtc cacgaccaag gtcgtccctg ggactcagac tcgtgaaatc gtcgaggcag
    24121 gacctcccct ttggcactgg tcgggaccgg aagtcctttg ccggcatcac gggaggtttc
    24181 gtttcctcca gacccggcgc agccgaaggg cgttggtgac caccgaaacg gagctcaggg
    24241 ccatggcggc ggccgcaaac atcgggttga ggaggacccc gaagaacgga tacagggctc
    24301 cggccgcaac ggggattccc aggacattgt agaagaaagc ccagaaaaga ttctgcttga
    24361 tcaccctcat ggtgagggag gacaggcgga tggccgatgc caccatccga agatcgtttt
    24421 gaatcagggt gatgtcgctt gcctcgaggg ccacatcggt tcccgcaccc acggcgatgc
    24481 ccacgtccgc cacggcaagg gccggggcgt cgttgattcc gtcccccacc atggccacca
    24541 cttccccctg ttcctggagc ctccggatct cctgggcctt atccccgggg agaacccccg
    24601 ccagaacgcg atcgatcccc agttcgcggg cgaccgctcg ggccgtttca ggtcggtctc
    24661 cggtaatcat ggccacatga agccccattc ttttcaaggc cgagacggcc ggccccgccg
    24721 tttctcgggg agcgtccgcc agggcgatga ccccttgaat cctttgatcc acggccagaa
    24781 aaacacaggt tttgccttcc tcggccagtc tgtttgccgg gtcttccagg gcatcgaggg
    24841 aaatccccca ctgatcgagg agacgctggt tccccagggc cacggatctc ccctccacga
    24901 tcccccgagc gcccaaaccc gagagcccct cgaaggattc cacgggcagg ggctggatgc
    24961 cccgggccct tccccaatct acaatggcac gggccagggg gtgttccgac aggttctcca
    25021 cggaaatcgc ccatgagagc agttgggacg gatccactcc cggcaaggtc cgaacctcca
    25081 ccactcgagg ggtcccctgg gtcaaggtgc ccgtcttatc gaaaacgatg gtcgtcagct
    25141 cgtgggccct ctccaggctt tcgccgccct tgatcagaat gccgttttcg gccccgatgc
    25201 cggtgcccac cataacggcc gtaggggtag ccaggcccat ggcacacggg catgcgatga
    25261 tcaggaccga gatgaaattc aagagagccc ggctgaatcc ggggttggga tcggcgaaat
    25321 accacacgag catggtaacc actcccacgg ccatgaccgc aggaacgaaa atggaggcaa
    25381 ccttgtcggc aaaccgttga atgggggcct tggaaccctg ggcatcctcc accagccgga
    25441 tgatttgagc caggaccgtt tccgccccga tcctggtggc tcggtagctg aaactgcccg
    25501 actggttgag ggtccccgca aagaccttgt cccccacctc cttggtcacg gggaggctct
    25561 cccccgtgag catggattcg ttcacggtgg agccccccgt cagtaggatt ccatccgtcg
    25621 gcacgcttcc tccgggtcgg accaggacca cgtctcctcg ccgaacctct tccaggggaa
    25681 gatccacctc gattccgtcc cgaagaacgg cggcggtctt gggtgtaagc ctcatcaacc
    25741 tgcggatggc ctcggaggtt cggcctcggg ccccgatttc caagaaccgt cccaggagca
    25801 ccaaggtgac gatcatggcc gcactatcga agtacaggtg cggtccatgg cccaggggat
    25861 cgacaaggtc cggtcgaaaa acgcccagag tcgagtacac gtaggccgaa aggctcccga
    25921 gggccaccag ggtgttcatg tccgcggttt tttgcctcgc agccttgagg gccccgtaca
    25981 ggaagcgtcg gcccacccaa aacaccaccg gcgtcgtgag gacaagcagg aaaaaggaca
    26041 tgacccggag aggaatgccg ctcaggaagg gaaaccattc ctgcatgctt cccatcatga
    26101 tccagacgtt tagaaaggct cccaccccga gcttgatttt gagctctctc ctttcctcct
    26161 gttgcgccgc ctcgatggga tctttccgga actcctccgc aagccccagg aaatcgtatc
    26221 cggccctttg gatgatcccc cgcaggtccg acaggccaac cgactccggg tcgtaggtca
    26281 ccgtagcccg ggaagtggca aggttcacaa ccgcgtccgc aacccccttc gccgatttca
    26341 gggcctgttc cacccttcga acgcaagcgg cacaggtcat tccccccacc aggagggtaa
    26401 ccttgcgcgg cccctgctcc ccgtcggaag gcagctccac gggttcgtag cccagttcct
    26461 ggaccctgcc cttgagggtt tccaaatcca caagctgggg atcgtactcg agggaaacct
    26521 tgcccgtggc aaaattgacg tcggcggacc gaaccccctc catgcttccc aggccttttt
    26581 ccacccgagc aacacaggcg gcgcagctca tcccccgaac atgaagactt ctcttaacca
    26641 acctcgtacc ccgccttcct gatctggttg cggacccact ccatgtccac cggctcggat
    26701 tcttcgaagg tcgcctcccc ccgagagaga tccacttcga ggtccttgat gcccgcaact
    26761 tcgttgaggg ccttcgttac cgccatcacg caatgctgac aactcatccc cttaatcttc
    26821 acggatttca ttggaacaac tcctttcctg cctggaccgg actcgggccg gtcataaatc
    26881 gtttccgggc cgccccgaac ggaacacggg attgcgcgag aacgcacggg agaaaacttt
    26941 cccggggaaa accccgggcg gctccccgga gcccccaaaa tccccctaac cggaagcaga
    27001 tcgggcccta ccctcttgcc ttgttggcgt tttttgtgcc caaccgcagt tcatctctcc
    27061 ttccggggag atcccgccga gcctcgggca agaccctcga gagaaagccc atagtcggtc
    27121 gagggcacgg cacgcatgcg gatcctcgtt gcccggcatt ttgcttaggt cggtggcctc
    27181 gagcccaggc tcgcctcgag gtccctcaca tcggtcacgg gggttcggca agcgtagttc
    27241 tcacaggcga ataccgccgt cttgcccgcc agggagcgga gaggttccac aaagggcgca
    27301 atcttcgtca gacgtccgtc gtcattgtcc gtggcccgaa acatcaggac ggagcggggt
    27361 cggaaggccc tgtgcaaagt tcgaatcatc tccccggtct gcgcctcccg ggaatccccg
    27421 accaccacca tttcccgggt cgggcccagg gcaaaatcca ggccgatcaa gaactgagtg
    27481 taccccgaag ggtactcctc caccagcggg ccgaagcggc gaagacaacc ctctgccgct
    27541 tggtcgaagt ccgtattccc cgtcatgcgg cccaatcgca gcaggttcaa cagagccgcg
    27601 gcattgcccg acgggatcgc cccgtcgtaa atcggtttgt cccggacaat gaggctctcg
    27661 gcatcttttc cggtaaagaa aaaccctcct tccctctcat ccccgaaaag atccagggtc
    27721 tgccgggtga ggttcacggc ctcctccagg taccgcacat cgaaggaggc ctcgtacaat
    27781 tcaatcaatc cccaaaccag gaaggcatga tcgtccaggt aggccctctg ggccacttct
    27841 cccagtcgat agcggcggta aagcctcccg gaatcgtccc ggagcacggt caaaacgaaa
    27901 tccgcagccc gggctgccgc atccaggtag gcggaatttc ccgaagcctg aaatcctttg
    27961 gccaaagccg ctatcatgag cccgttccag gatgtgagga tcttgtcgtc cttgagagga
    28021 tggatccgtt gttcccgggc agccaggagc ttttgcctcc ccgattccag gattccttcg
    28081 agctcatgca ccctcatccc aatcatttcc gagaaaaccg aggccggttt cgggatgtgg
    28141 ggaatgctcg acccctcctc gaagtttcct ccgggggtga tgccgtagaa acggcagaag
    28201 aggtccccgt cttctttccc caaagcagcc aagacctcgt cgggggtcca gacgtagaac
    28261 tttccttcct ggccctcgct gtccgcatcc tccgccgaat ggaaacctcc ccggggatcg
    28321 gtcatgtccc ccagaacata ctcgaaaatc tcccggaaca cccgggcata gaactcgttt
    28381 cgagtcagct gataggcttc cgcgtaggcc atggaaagca tggcctggtc gtagagcatc
    28441 ttttcgaagt gagggacgag ccagcgctgg tccaccgagt agcggtgaaa gccgagcccg
    28501 atctggtcga agatacctcc ccgacgcatg gaatgaaggg ttcgctcgac gatatccaga
    28561 gcgtcgcttc ggggtccctg cccgtgtcgg cgaaggagga aggtgagatg gtgcggtgtg
    28621 ggaaacttgg gagcccctcc aaagccgccc cattgaacat cgtggatttc cttcagctgc
    28681 tgttgagctc gtgcaagaac ctcttccttc gggaccgccg aagacccgga aaattcccgg
    28741 ggctgaatgg cccgggtgac ttcttctccc gcctgccgga cccgatcggg ttgagcctcc
    28801 caaagatgga ccacctggct gaggatgtcc acaaaccccg gcatccccat ccggccgtgc
    28861 ttggggaaat aggtcccggc gaaaaacggc ttttggccgg gcgtcatgaa cacggacagg
    28921 ggccaccctc cgctccgatt caacgcctga caggcgctca tgtaaatgtt gtccaggtcc
    28981 gggcgttcct cccggtccac cttgatggaa acgaaatgct cgttgagcag ggcggcaact
    29041 tcccggtctt caaaggactc gtgggccatc acatggcacc agtgacaggt ggcgtaaccg
    29101 atggaaagga agatgggctt gttttcccgc cccgccttga gaagggccgc ctctccccag
    29161 ggataccagt cgacgggatt gtgggcatgc tgaagcaagt aaggactctt ttcgtggatc
    29221 aggcgattgg gcatggcggc aactccggaa gggcgggaac ggatgggacg atgaaggaaa
    29281 ggcgtgaaga atcctttcct tcatccagga agtcagccca acaggagacc acaaggcggg
    29341 ctgaatgcaa accggttaaa cggcggacgg gggagccgaa ggccgggcgg aattcctccc
    29401 cgtcatgcgg atacttcggt cttgcacttg gaaaagtcca tttccttctt acactgcgga
    29461 catatgtgcg gtttgctgaa ctcatccgag aaaatttcct tttgcgcccc gcattcggag
    29521 cacgtgcaaa taaacgaact caagtgtttg aatctctcaa aacccggaca atgtgcaggt
    29581 gtcgacatag ccttcccctt tctttgacag caggttctcc atcgcgtccc caaagacgct
    29641 tccctccaca aagactcagt ctttctcctc ctccaacacc agggtccctt tgtgcttcgt
    29701 tccgcaggaa ggacagagaa tctctatttc ttgttccgga ccgatgtact tatctttcat
    29761 cgtctcaggg ggaatgaaac tcacatcgcc gcaagcacat tgcggacact ggccctttac
    29821 caaatgtctc tgcttctttg ccatgatact cctccgtgaa tctttgaaat ccgtcgtcct
    29881 tttccccttt ccatgctccg atgatccgtc gaagaggggc cgattatggg agcttccgga
    29941 gctcgtcgat catcgcttca aggtttggcc tccggttgat gtcgtgcaca tccatgtatc
    30001 gaatgattcc ctgcttgtcg atgacgaaaa cggccctctc ggaagtgcct tccgatcgaa
    30061 ggaccccgta cttggccgcg acggccccat ggggccaaaa atccgagagc accgggaacc
    30121 agactcctcc catctgttgg gtccacgcat ggagggtggc caggttgtcc acggtgatac
    30181 ccaagatcac cgcctggtat tggtcgaaat aatccttcac gagattgtac ccgggccatt
    30241 gttccgaaca caccggggtc caggctgcgg gcacaaagga aagaaccacg ttcttcttgc
    30301 ccagaaaatc cgagagggtc accttttccc cggacaaaga aggaagagtg aaatcggggg
    30361 cggggtcgcc cactttgagc tgggtccggc tgtctagggg tttgagtggt cctcccgagt
    30421 agatcttccc ctcgaatttt tcggacacgg catgggaaag tgcggtattc cagcagaaca
    30481 cggccaccag ggccaccagg aatcgtgcgg gcttagaatc gatcttcatg gatgcccctt
    30541 tcagttccgt ttgcctttgg aaaggaggag attcaggaat tcttggggat cgccgaaagc
    30601 tcccgccttg gagtagatga cttggtgaga accgtcctgc atcatacgaa tcacaatgaa
    30661 atacggggtc cgcacgtccc cgaaagtgcg gtgcaggttc aaatcgttgt ccgggaccag
    30721 gggaaagggc accgaaaact gcttctggaa ggtcgctact tcgaaagaag tatttcctgc
    30781 cccgatcccg acgatcttta cccggtcctt caaatccggg ttgccggaaa tgagccggta
    30841 gagttcattg accacgggag cttctttctg gcaatagggg cagtacatgc tgaatatctc
    30901 gaggatcacc agttctgcgc gaacctgggg aatcttgaac gacccttccg cccccactcc
    30961 caggtactgt tgatgagccg ccgggtccgg aagggtgagt ttgacgtccg gaagcacccc
    31021 tccttcctcg gggggaccga tcgcctcgga ggacgccccc ggagccggaa tcagcattcc
    31081 caccgcaaca gcacccacca gcacccacca tccgatccgt ttgaatccgc ccatggaatg
    31141 tcctcctcca aagttccgct caccgaacga tccgtccaat ttcccgggaa tgcttcctgt
    31201 cgcttcatga atacacgacg acccatctcc gtacccgatc ctcccctccc ccggactgcg
    31261 gcgggaaccc aagcaaaccc ccgacggccg ggccccgtcg ccagaccacg aggaacacga
    31321 tgagtcgacg accggaacgc tcccgccttt cccaaaaccg gatgaatcgg cttctcggca
    31381 ggtgggttcg aaaccccggt tgttgacaca gccgcatccg ccccaagccg gacccggttc
    31441 ccggcgggcg ccggggcccg ataggcgtca tgaagtctcg aatccgacca ttgcgggaat
    31501 aacgccgggg gtctacctca aatccatcgg ggtcgaagcc cccgaaaccg ggagatcgct
    31561 cggcccgggg gaaatccctt gtcgtgcagc gttccgtccg tccatcccgc gggcaagtct
    31621 caggcaagtt ccccagcccc gtccagaccg gcctttcctt tttcaataaa ccacccttcg
    31681 gggtccgtat agtagaattt taggtcggcc aacacaatgc agtgctgacg ctcttcgccg
    31741 atcagtctct cgagaagttc tcgcacccct ttgtcttcgg ccgaccgagc ctgcttgtca
    31801 agaaaggaaa tgctggcatt ctcgagactc agccccgttt caatggccgc cacgtcgtcc
    31861 aggcaggccc ggggcatcct gccgtgttcc cgggcgatcc ttctcaagag tgattttttg
    31921 tcctcgccgt cgaaagggac aaaccggcat gcgtccgcgg gaacagagcc cttcttgagt
    31981 tcctcgtagg ccgcctggag gcttcgggcg tggtcctcct cggcatcctt gagcatgcga
    32041 aaggtctcga ttcccacctg gtccgggcag gtcttcatgg ccccctcgta gagggccttc
    32101 ttcttctctt tcatctcgat ggcggtacag aatatcgtca ccatggggtc ttgattcgta
    32161 tccatcctcg taccctcctt ctaaagtaaa acccagtcaa aatcaaatct tccgcttatg
    32221 ctaataccgc ctctccaccg tgtccattcg ccgtccgact tttgatggga tcggacgcct
    32281 ctcaagactt gcccctggtt ttcgatccgc attttcagcc cctcggtcct cccctcgaag
    32341 gtggagacgc ggaccttgac agccccgagg aatcgattaa tttttggatg taaaacgcaa
    32401 taagtatcgg aaatgttgag tgaaaggagg ttcgagccat gtttgagttg accccgtttc
    32461 gaaaacaacg atccgtaacc aatgctccgt tggcctggga ctgggctgat cgtctattga
    32521 acgagttcat gcctttccga ctctttcgtg agatgatgcc ctccctgtgg agggaacagg
    32581 gggcactcat gcccgccttc gacattacag aggcggagga ccacctggtc gtgagggcgg
    32641 atctgcccgg catcgacgcc cgcaacctcg acatcagcct ggcgggaaac gttctcacca
    32701 tcaagggaga gaagaaggac gagagatcgg agcagaacga gttctaccat ttttgcgaga
    32761 ggcagttcgg ctcattcacc cggtccttca ccctcccctc ggatgtgagg gaggagggaa
    32821 tagaggccac ctacaaggat ggtgtgctcc aggttcggat ccccaagacc gaacgaaccg
    32881 aaaagaagag gatcgaggtg aaaacccatt agagacgtac cggcaaatgc aagatttccc
    32941 tcacccggac ccactcccac cgacgggggg agtggggacc ctcttgcctc gatagaggag
    33001 agtatccgga tgaggaagag aaaaccttcc ggaaatcggt tcaaaagaaa ggacggggag
    33061 agactccttc cccgtccttg ccgttatgcg ctgtaaccgg aatcccccct agacgctttc
    33121 gatcatggca aacacctggg cgtcgtcaaa accgtggagg cggatggccc ccgagcggca
    33181 ggatgccgtg cagaggccgc agcctttgca cagggccggg ttgatttccg actttccgtg
    33241 gacgttcaga ctggctgcgg aatagggaca cacggttacg caggttccgc acccgctgca
    33301 cagaaactga ttcgtctcgg ccaccgcccc gctgaccagg acctttcctc gggtcagaag
    33361 caccatggca cgggcactgg cagccagggc ctgggcgatg ctctcgtcca ccgctttggg
    33421 gtaatgagcc agtccgcaga cgaagacgcc gtccgtggcg aaatcaacgg gtcgcagctt
    33481 ggcatgggct tccacgaaaa acccgtcgga attcaggggc actttgaaga actgggacag
    33541 cagttcgttt tccgagggaa caatggcggt ggccaggacc agcagatccg gctcgatggc
    33601 cagggggcgt cccaggatgg ggtccgttac cgtcacctgc agggccgatc cttcagtacg
    33661 aacctgcggg cgattctcgg ggtcgtagcg cacgaagacg acgccctcct tgcgagcctg
    33721 actgtacagg tcttcccttt cgccgtaggt gcggatatcc cggtagagta cgtagatatc
    33781 catgtcctgg ttgcgccgtt tgagctcgag ggcgctctcc atggaatgag tgcagcagac
    33841 ccggctgcag tacgggcgat tcgcttcccg ggaaccgacg cactggatga acactgcaga
    33901 tcgaatctcc ttgagggatt cgtccccttg gatgaaccga gcgtcgagtt ccaggtgagt
    33961 caggactcga gggtcttttc cgtacaggta gtcgtcgggt ttgaactcac gggctcccgt
    34021 tgccaggatt gtgatcccgt ggcccaggga gaagggatct cccttctcgg gccgaatcgt
    34081 ggtcacaaaa ttgcccacga atccttcgac gttcacgact tctgcagaac gatgaatgtg
    34141 aatcagcgga tttgcccgca cctgctgttc gagatcgtac aggtaggatt ggacgttctc
    34201 tccccgccat gttctgaaca aggatctcgc cgcacctccc aattcggcgg tccgttccac
    34261 gatgtggact tcgtaaccct gctcggcaag cgtcctggcg gccatcatgc cggcaatgcc
    34321 tcccccgatg acgagggaca cggggttgag gggcacttcg gtatcctgga ggggctcgag
    34381 gagggtggct ttggccacgg ccatgcgaac caggtccttg gccttttccg tggcggtgac
    34441 cggatcgttc ttgtgcaccc aggagacctg gttgcggatg ttggccatgt caaagagata
    34501 gcgattcaac cctgcgctga cgagggtttc ctggaacagg ggctcatggg ttcgaggggt
    34561 gcatgcggcc accaccaccc ggttgatccc cttctcccga atgacttcgg ccatcttggt
    34621 ctgggtatcc tgggaacaag agtagaggtt ctcctcggca tagacgacga agggaagtct
    34681 cttggcgtac tcccgtacct ccggcacgtt gaccactcct ccgatgttga tcccgcagtg
    34741 gcagacaaag acgcccactc gagggacttc tccccgaatg tccgtctggg agggcgtact
    34801 gggttcccgt gtcacggtcc agcgaacgtc gttgaggagg acgccggagg acgccgccgc
    34861 cgccgaacct tccaccacgg cctgtgggat atccttcggc ccctggaacg tgccgcagac
    34921 gaagatgccg ggccgggagg cggcaacggg agcgaagctg tcggtcttgg caaatcgata
    34981 ctggttgagc tcgatcccca atcgattggc caggtccaac agttcctgcc ccactcgaaa
    35041 tcccacggaa agcacgacga ggttgaagac ctcccgttgc tgccggccgt cttctcccac
    35101 gtattggatt tccaggtctc cgtctcccac cggggaaatg gaatgaactc gggaacggat
    35161 gaaacgaacg ccgtgctctt ccttggcccg ctcgtagtac tgatcgaaat ccttcccgta
    35221 ggtacgcata tccataaaga aaatggcggc atccatgggt ccgtgggcat gttccttggc
    35281 gatcaccgct tccttgaccg cgtacatgca gcagacagcc gaacagtagg gatttcccgc
    35341 cacgttgatg tcccgggacc ccacgcattg gagccaggcg atcttcttgg gttcttcccg
    35401 gtcataggga cgcatcaggt gtcctcggaa gggaccggac gcactcagga tgcgctcgaa
    35461 ttcgagactg gtgaccacgt tgggaaggcg agcgtagtca taggtatcgt attcggacgg
    35521 atcgaacacc tcgttgcccc cggccaggat caccgctccc acctcgactt ccacccttcg
    35581 cagggtatcg tcgaaccgga cggccttggc caggcagacg gtttcacaaa gtccgcaccc
    35641 cacgcaggaa tccgggtcga tggagtagac caggggcacg gcctggggat agcgaatgga
    35701 ggtggccgcc cgggtatcga gccccgcgtt gaactcgttg acggcgggga ccggacatac
    35761 ctgggcacag actccgcatc ccgtgcattt ttccagatcg atgaaccgcg ggtagctctt
    35821 gagaagggcc ttgaagtgcc ccgcctcccc ctcgattgcc tcgacctcgg cgttggtgat
    35881 gattcgaatg tttagatgcc ggccgacctc gaccagcttg ggagagatga ttcacatggc
    35941 acagtcgttg gtcggaaacg tcttatccaa ctgggccatg agacccccga tgcaggaagc
    36001 tcggtcgacc aggtagacga agtatcccga gttcgccaga tccagggcgg cctgcatgcc
    36061 ggcgatgccg gcgccgacca ccatcacggc tcccactttc tcttttcgtt ccgtatgatt
    36121 catggccatt cctccatgtt cggtgtccct tgccgatgct cttcccgggt gaaacgcccg
    36181 cggcttctgc ccgttgcatg ggaagattcc gcagggatcg acggtcccgg accccgccgg
    36241 gggccgaggc cggacgactt atgcagccag gcttatgaac cgcggggtcg tgccttcgta
    36301 gcccttcagt tccacctgcc cggccttttc gaggtccgtc agtcgcaggg aaacttcata
    36361 gacgctcaac ttggttttcc gcgagatctc ccgcacggat tgagcgccct ccttcagtac
    36421 ctccaggatc aacgccttct ggtactcgag cccggcaacg gtcttgagga cctgtttgaa
    36481 ttgatcttcc tccagcttcc tgtggtacac gttttcccgt tccgtgagct ggcgctccat
    36541 tcccgtgagc cagcgaaacc gtacggaatt gagcactccc gccaccgccg ccaaggggag
    36601 actgaaggtc tccggatcaa agggccccag cttccgggtc cgctcggtca gctccgtcac
    36661 gtagtcggcg aagagctgtc cctcggcagc gctgacccat cgcacttccg ctcgatccgc
    36721 accgactccc gacaggtcca gcagccattt gaccacgtcc attctcttgg ccgtatacag
    36781 gtttccgtcc acgtagtggc agtccccaaa gtggcaaccg aagaccaaca cgcagtcgta
    36841 gccgctcatg agcccctcca ctacgtggac cgggtccacc cgacccgagc acatggtccg
    36901 gatgatgcga atggtgggag gatattgaaa tcgggatacc ccggcaaggt ccgctccggc
    36961 ataggaacac cagttgcaca ggaacgccag tattctcggt tcgaatgctt cttccatctt
    37021 gtggctcctt tctccggtct acgccttttt cccacccgct cggatggtcg cgacgatctg
    37081 gctgtgacga aaatgcagca tgtcgatggc cttttgggga caggcggcgg cacagactcc
    37141 gcaccctttg cacgaggcgg gaatggattc ggccacgtat cctctcccca ccacctcgta
    37201 gcgccggatg gctccaaagg ggcaggactt ctcacacaat ccgcatccca cgcagagctc
    37261 cgggtcgatt cgagagaccg tgggttcgag cttgaggtgg ggctgtacga ggatcttggt
    37321 cgccttgctc gccgccgcct gggcctgggc gatggattcc tcgatgggtt tgggacaatg
    37381 ggccagaccg cacacaaaga ttccctcggt ggaaaactcc acgggccgca gcttcatgtg
    37441 ggcttccaga aagaacccgt cctggttcac ggggaccttg tagatccggg caagctcttt
    37501 tagaccccgg ggaatgacgg cggtggccag gttgaggtag tccacctcga gtcgcacggg
    37561 ccgatccagc acatggtccc tcacagtgat ttgcagcttg ttcccggagg cttccacccg
    37621 cggcttttca tcccgggagt agcgaatgaa caggactccc tcccgccgag cctgggtgta
    37681 gagctcctcc cggaggccgt aggtgcggat gtcccggtag aggacgtaga catccaggtc
    37741 gggcttgcga gttttcaaat cgatggcctg tcggatggag ctcgtgcagc ataccttcga
    37801 gcagtaagga cgatcgggtt cacgggaccc gacgcacaag aggaaagcca cggcttcggc
    37861 ttcttcaagt ttctccggct tgcggtcgaa gaggtcctgg agctcgtgcc accgggtgac
    37921 ccgctcgctt tccccgaaca ggtattcttg gggctccagg gtctcggcgc ccgtggcgag
    37981 aatgaccacg ccgtggttga ggtctcggga tttcccattc acggagatcc gggtggtgta
    38041 ctgacccacg aagccctgag cgctttccac ccgtgcctcg gtcaagacct cgatgttggg
    38101 atgagcgaac acccggtggt tcaggtcgtt gaggtaccct ccgacctctt cccccttcca
    38161 ggtggatcgg atgtgtcgac cgtgtccgcc cagcaccggc tgccgctcca ccaggtagac
    38221 ccgcaatccc tggtccgcga cggccagagc ggcattcatc cctgcaatgc ctccgccgat
    38281 caccagggcc gagcgccagg tttccgcctc gagctcttcg atgggctcga ggagggccac
    38341 ccgggccaca gccatgcgga ccaggtcctt ggccttctgg gtggctttcc ggggatccgc
    38401 catgtgaacc caggaattct gatcgcggat gttggcaaga tcgaacaggt accggttcag
    38461 tcccgcctcc cggatggtct cctggaacag gggctcgtgg gttcggggag tacaggccgc
    38521 caccacgatt cgattcaacc ggtggtcttt gatggcctgt cggatcaacc cctgggtgtc
    38581 ttggctgcag gtaaagaggt tgtcggcaac ataggccacg ttggggaggg tcgcggcata
    38641 atcccgcacc gccgggacgt ccaccacccc cccgatgttg attccgcaat ggcacacaaa
    38701 gacgccgatg cggggttttt ccaggataat gggatcttcg gggggatagg tcttctcatg
    38761 ggtgagagta ccccgagcat cggccaggga agccgacgag gctgccgagg ccgccgaggc
    38821 ttccatcacc gagtggggga tgtccttggg tccaaggtac cctccgcaaa cgtagacgcc
    38881 gggccgagag gtggtcacgg gagaaaagga gcacgtagag ccgaagccgc tctcatccac
    38941 ccggattccc agcttcccgg ccagttctat ggtgtcggcg gacaattcca tgcccacgga
    39001 aagcaccacc aggtcgaact cctcactgtg ggtccttccc gcgtcgtcca catactccag
    39061 gcttagctcg tgggaccctg gagacgatgg acgaacggag tgaacccggg aacggacaaa
    39121 acgaactccc acctcttccc gggcccggtc gtaataacgc tcgaagtcct ttccataggt
    39181 ccgcatgtcc atatagaaga tcgcggtatc caggtccgcc cccgcgtgct ctttggcgat
    39241 caccgcttcc ttgacggcgt acatgcagca aacgcccgag cagtaaccgt ggccgccgtg
    39301 atggaggtcg cgggacccca cgcattggag ccaggcgatt cggcgggggg ccttccggtc
    39361 cgaagggcgc agcaggtgcc cttgataggg acccgaggcg ctcaggattc gttcgaattc
    39421 catgctcgtg atcacgttgg gatgaacccc gtagccgtag gtgtcgtatg cggtgggact
    39481 cagggcactc aacccgggcg ccaggatgac ggaacccacc cggagttggt gcacccggcc
    39541 ctcctgatgg aaatccacgg caccggccgg acagaatttc tcacaggccc ggcatttgcc
    39601 cttttcaaag tagatgcaat gctcccggtc gagggcatac ttgaggggaa ccgcctgcgg
    39661 atagcgaatg taggccgctt tgcgtttgtt gagtccctcg ttgaattcgt cctccacccg
    39721 gcgggggcac ttttcggcgc agactccgca ggcgatacac ttttccgtat ccacaaaccg
    39781 gggctcttcc ctcaccgtgg cgaggaagtc cccctccttg ccttccaggc tttccagccg
    39841 ggcattggtg atcaattgaa tgttcagatg tcggccgcac tccaccagct tgggagacaa
    39901 gatgcacatg gagcagtcgt tggtgggaaa cgtcttgtcc aggcgggcca tgattcctcc
    39961 cagggaggta tccttttcca ccaggtagac gaagtagccg gaatccgcca ggtccagagc
    40021 ggcctgggct cccgcgatcc ccccaccgac aaccaatacc gcggccgtca tgggactcgt
    40081 attcgatcga tcttccatgg atatccctct cctctgttcg ggggattgca ccccgttcgt
    40141 caccggggac ccgggaactc gacgcgttct cttgcattcc ccggtccccg cttcccttta
    40201 ggattgcggc ttgcctcgtt tcctgagaca aatgcagtag aaagaatcct ccttggcggg
    40261 ttttagatgg atgaactcga aggaggaagc gggaacgatt cttaagagat ccgccaaggt
    40321 ttcgggccca cagccgatga tttccatgat ctcgttgacc ttcatctcct ccagttcttg
    40381 ggtgagttga agaagagcaa tgggagcaaa gctgcctcga agatcaaaaa tgtggtcgac
    40441 cttctttttc aaggctttga ttctccttgc aaaatggccc cgatcgggtt ataagcaatt
    40501 acagtgccag gaaaaccggg ggccgtatcg cacccagtag cgtcgagaac ggcggcggaa
    40561 ttgtccctcc ggagacggcc ggcgtctttg cagtccggtc ctgcgaactg gcgacggttg
    40621 ccgtacaacg ccggtctatc ccgggatcca ccccctgttt gccacaatcc ggcacgattc
    40681 ttgatcctgt taaagaacac tgaataggga tctgaaacat ctgaacggtt gatcactcga
    40741 gagttgaaca gtctccagtc cgcgcgccat tccagagaac agcctcagat tccgtttctt
    40801 ccgcgtacag gccctttcaa ccgggttttc ttgcaggtgt taagtgttaa gttttaacca
    40861 cctgttaagc tttaacaggg gaccaacgat ggccgaacgt gcactggacc gatactggaa
    40921 agccgtcgtg agcaccattc gggacggcat catcatcgtg aatccccagg gcaccatcat
    40981 ctcggtgaac aaggccctgg agaacatgac gggctacagc agggaggagc tcatcgggaa
    41041 gaagtgttcc atcatggagt gcagcctttt cgagaaggcc cgggaacaaa gaggggagca
    41101 ctggtgcgtg ctgttcaact ccggagacct gaccagccgc aagtgcaccc tcttgcgaaa
    41161 agaccggacc atcctccatg ccctcaagag cgcctctctt cttcacgacg accacgggaa
    41221 ggtgatcggg gccgtcgaaa ccattaccga catcaccgaa ctcatcgaaa aggacattca
    41281 gatcgcggcc ttccggcgag agctccaatc cgaagacggc ttccagggcc tgatcgggac
    41341 ctcggcaccc atgcagaggg tctttgatct catcgccaac gccgcccagt cggacgctcc
    41401 ggtgatcatc tttggggaaa gcggcaccgg gaaggaattg gtgagcaaag cgatccacaa
    41461 cttgagcgcg cggagtcaga agcccttcat caaggtcaac tgtgccgccc ttaccgaatc
    41521 tctcctggaa agcgagcttt tcggccacgt gaaaggcgcc tacaccgggg cctatcgaag
    41581 cagggccggt cggttcgaag ccgcccaggg aggcgacatt ttcctggatg aaatcggcga
    41641 tctgcccctt tccacccagg tgaagctgtt gcgggtcctc gaggagaagg tggtcgaacg
    41701 agtgggggac aatcggccca ttcccatcga ggtgcgcatc atcaccgcca ccaacaagaa
    41761 cctgctgcaa ctggtggaaa aggggctttt tcgggaggat ttctacttcc gcatcaatgt
    41821 gatccccatt cacctgccgc ccctgcggga aagggtcgaa gacattcccc tcatcgccga
    41881 gtccttcttt cggaaaattc ggctaaaaag cgataagagg gcggagggga tcagcgccga
    41941 caccatggaa gtgctcatga actacccctg gcccggcaac attcgggagc tcaagagcgc
    42001 cttcgagttt gccttcgtca cctgccagga aaggctcatt caaccctggc acctgcctca
    42061 gaacatctcc aagtaccggg gtcaggccgg tcgcagcgga cccaaggtcc tcgacagtcg
    42121 catggacatg gagaaacgtc gactcctcgc cgccttgtcc caggccgggg gcaaccagag
    42181 cgaggccgcc cgcattctgg gcgtgtcccg ggtgaccgtc tggaaccgga tgaagcggct
    42241 gggaattgcc aagggaaaaa tcttcaacac cggttcgacc taaattccta ccgaatgcat
    42301 ttgccaagtt ctttgccgac cgcggttgtt ttccgcgggt tgggggtctt atcttcatcc
    42361 tggacctcga cacgtcacgg aaggagatca tgactaaaga acaccaatct tcaagccgga
    42421 gcgaaggctt cgtccgcagc gacctggaaa acgagctcaa ggccgtcttc gagcgactcc
    42481 cccatcccgt ctccgtctat ctcgtggcgg gaagggccgg cgaggacgtg ctcagccggg
    42541 ccgccaggga gctcattcgc ggctttcagg gcctgtcgga caggatccgg gtgacggaag
    42601 tggaaccccg cgacgacctc gcccgcaagt ggaaggtgaa cagcattccc accttcctgt
    42661 tcgatcccga gcattattcc atccgatacc tcggcgtgcc cttcggagaa gaagcgagga
    42721 ccctgctggg catcctcatt ctcctgggct accgatccgg caacttgacg gaacagtccg
    42781 ccagggtcct tcaacggatc gataccccca gaaggatcaa ggtctttgtg agcgccacct
    42841 gcccatactg ccccgaacag gcgatgaacg ccgctcgggc ggccatggag aagcccgatc
    42901 tcatttccgt ggagatcatc gatatccagt cgaaccagga gatggccaac cgatactcgg
    42961 cattcagcgt cccccaggta tttgccaacg aggtcctcat cgcccagggg gcccagtccg
    43021 aagagttgtt catgctgtcc ctggaaaaga tggagcagca gaccctgttc attccggaaa
    43081 gcgactccga ggaagtcgaa acggacctgc tcattgtggg gggaggtccc gcggggctca
    43141 cggcggggat ttacgccgtt cgcagcggtc taaagaccgc cattgtcgaa aaggggcctc
    43201 tgggagggca gatcgccacc acgcccgttg tcgaaaacta tccagggttc acccgggtcc
    43261 cgggcaaggc cctggtggac atcctcgcca gtcacgccct ggaatacgtc caaatctttc
    43321 ccggggagga agtcgtcgag gtccggccgg gaaaccccat ccggattcgc accacccgga
    43381 ggaggttcaa ggccggggcc gtgattctgg ccacgggagc cacctaccgg cgcctgggcg
    43441 ttcccgggga aatggaactg gcgggccgag gcgtcagcta ctgtgccaca tgcgacggca
    43501 ctctgttcaa gggaaagaag gtcctggtgg tggggggagg aaacagcgcc ctcacggaag
    43561 ccctctatct atccaacatc ggggtctccg tcaccctcgt gcaccgtcgc gacgccttcc
    43621 gtgcccagga gcacctggtg aaaaacgtgg ccgccaaccg gatccccgtc ctctgggaca
    43681 cggaggtgaa ggagatccga ggccgagaac gggttaccca ggcgaaactt gtgaaccgta
    43741 agaccgggga gacgacttgg acggataccg acggcgtgtt tgtggccatc gggtacgtcc
    43801 ccaccgtgga cctggctcgg aaattgggcg tcgaggtcac ccccgacggg ttcatccgcc
    43861 acgatgccca tcaccggacc aacattcccg gcatctactc cgcgggagac gtggagggcg
    43921 gctacaagca gatcgtgacg gccatggccc agggtaccga agcagccctg tcggtctacg
    43981 aggacctgac ccatccttac tggaaacaag agggggctcc gcccggatga gattcctcac
    44041 cttactgcgg gccgttcccc aggacctccg ggggcatgat cttgagggaa gatcgtgcat
    44101 tgcagtgacc ggacaggcga cccaagggtc ccgtgttcgg aaagaccaag agcaaccaca
    44161 gggagcgaaa ccatgttctg cgtcgatctc agcgccgagg aaaccggagt gctgcaacgg
    44221 gttccggatt cgtacctttc cgaattgaag gaagagatcg ctcacacgga tcgcagggaa
    44281 tcccgtcaaa tgctccgggg tgaagaagaa gtggtgaaac gcgtgatccg cgaactcggg
    44341 tccgaagcct cgtccgagat tcactgagtt gcttgaacga gcacgcgggt atcggggggc
    44401 gaaatcaacc ggggtgattg gttcgccccc tgtcgtttcc ggggttggcc ctgctcggcc
    44461 caaacgcggg cgggggaggg aggccgggac ggcttgtcgg ttcaaggcgg cggcagccga
    44521 ccgaattcgg gaccgggaga ctcttccggg cccggcggaa aaacacccgt taggaagaag
    44581 ccgggtcctc ccggggcggt tccaggatca tgcggctgtc cgccacaacg gcccctttgc
    44641 ccttctcgta ggccaccagg ggattgatgt ccagctcttt gatctcggga tggttgaggg
    44701 ccatgtccga gaggcttacc agcagccttt cgaggagctc gccgtccacc ggcggttttc
    44761 cccggtaccc gtccaggacg gcatagccct tgatcccccg aaccatacgc cgggcttcgt
    44821 ttcgaccgat gggtgccagg cgaaagatca cgtctttgaa gacctccacg aaaatgcctc
    44881 ccaaaccgaa catcagcaac ggtccgaaac cggcatagcg actcacgccg agaatcagct
    44941 cttctccggg gtccgccatc ttttgcacca ggacacccgt gactcgggcc gcaggcgcca
    45001 ggttcctcac gttctccatg atgcggtcaa acgcctcctc ggcagcctcg cgtccctgga
    45061 tatgaagctc cactccgcgg acatcggact tgtgcaggat ctggggcgat acgattttca
    45121 aggccacgga ggatccgatc ttttccgcaa aatccccggc ttcctcccgg gttacggcca
    45181 ggtaggtggg aaggacgtcg aagccgtagc agttcaggat ttcgtgggtg tccgcttccc
    45241 ccagccgggt ctttccctgg tcgagacatc gtttaatggc ctgggcggct cgatcccggt
    45301 catgagtcag gctgaactgg gccaggtgtt gacgattgag ccaccgggag tatcggtaga
    45361 gggctccgta ggcctgggcc gcatgctccg gaaaacggaa gaccgggatt ccccgctcct
    45421 gaagatactt caccccggag gaaacatcca cgatccccat gaagcagcaa agaatcggct
    45481 tggcggtgtg gagggcgatc ttggcgatgg cttcggcagt ccccagggcg tcggtcatgg
    45541 attggggggt gaggatgacc agggctccgt ccaccccttc gtcacggatc accgccccca
    45601 gggcgttctg gtatcgatcg atggacgcgt ccccgatgac gtccacgggg ttgtggaggt
    45661 tggccgttgc gggaaggtga ctggccaggg cttccaccgt ttcttcatgg aaccgggcaa
    45721 gggtgagccc cgatgaaatg gtcatgtccg ttgccacgat cccgggacct ccggcgttgg
    45781 tcacgatggc cacccggttt cccgaggggg tcttcttgct cgcaaaggct ccggcaaaat
    45841 tgaagagttc ctcgatggaa tcggcccgaa tgatcccggc ttgctggaac acggcgtcgt
    45901 aggtcccttc ggaccccgcg agggaacccg tgtgcgaggc ggcagccgcc gctccggccg
    45961 cggtccttcc ggacttgacc accagcacgg gggtcgggcg gtctcccgag gtgattcgct
    46021 tcaccgcttg agtgaactcc gctccccgac gcagctcctc gaggtagatc atgatgacct
    46081 cggtgtcctt gtcttcatga aggtaccgga gaaggtccag ctcatccaca tccgccttgt
    46141 tgccgatgga aatgaacttc gaaaagccga attcccgatc ggcggcaaaa tccaagaccg
    46201 ccgtacacag ggccccgctc tgggagatga aggagatgtt gccgaaggcc ggcatgcgcc
    46261 cggaaaaact ggcgttgagc ttcaccgacg ggtggggatt gatcactccg aggcagttcg
    46321 ggcccaccag ccgcatcccc gcatccttgc agaccgctgc gatccggtct tccacggctc
    46381 gcccctcggg accaacctct cgaaaccctg ccgacacgat gacgatcccc tggaccccct
    46441 tcttggccgc ttcctcgacg gtcttcaggg attcccgggg agggagaatg aggatggcca
    46501 ggtccaccgg gtcggggaca tcggacaagg tgggataggc cttgacgcac tggatggccc
    46561 tggctgtggg attgaccgga aagagcactc cctggtatcc gcccttgagg atgttgacga
    46621 aaatgtcgtg gcccaccttg ccgggcttgg tggaagcccc aaccaccgca acggaacgag
    46681 gagaaaagat cgcatccaga gtcgtcatgt ttcacccttc gtccagagag acttctcgaa
    46741 atcccgcggc taatccctta ggattttaac atgaatcttg cgggtccgag gcccgtcaaa
    46801 ttcgcagaga aagattcttt cccacgtgcc cagcaggagc cttccccctt ccacaaggac
    46861 ctggacgctg cctcccacca gaatggattt gatgtgggcg gccgagtttc cctccccatg
    46921 gagataggcg gccttccagg gaaccagctt ttccaacgtg ttcgccacgt cctgcatgac
    46981 cgcggggtcc gccccttcat tgatcatcac tcccgcagtg gtatggggaa catacacgat
    47041 gcacaggccg tcggccactt ctctttccct caccttcccg gcgacaagat ccgtgatatc
    47101 caacagcgcc gtacggtccg gggtgcccag ttgaatggtg tcgatcatgg cttcctttcc
    47161 tcaccggggg agaatcaaac cggattccga caggtagaag ggttccgcct tttcgccctt
    47221 ttcaccggcc tggtcggcct tttcgaattc ctcgggccaa taatgaccca tggacaggat
    47281 caccatttga cgaaccacgg gattgtcccc ggcggagccg gtccccgcca ggtgccgggc
    47341 cacgacccag caggatcggg ccggttcttc ccggtccgag agcttgaacc atagagcctg
    47401 ttcttcaaaa aaccgctggt agaagcgccg caaggcaccg gtgcagatct cttcggccgc
    47461 tttccccacc atttccaggg tcctttggta ctggatctca ttgggaacca ccaggaccgg
    47521 cctctccaaa tccaggatcc tctgccagta gggctgcaaa tccttctggg ggaaaaaaag
    47581 ccagctatag ggcaccttct ccaggaggcc catcacgtcg atgccgttta agaagcgttc
    47641 ggggttctcc acgtcggcca tgaggctgta ggcatacggc ttccgctccg gttcgaaaaa
    47701 gggctggaaa agccgctttg ccgtatcggc ctccggcgtc cttccccttt ccccgcaata
    47761 ctccgccatt tccatgaaca aacgggccgc atgccagatg gggatttccg ccggcttgaa
    47821 atcggaggcc tcggtcagca tctgaatata ctcccggtag gcccgattgg agctctcggt
    47881 cacatgaacg ctcaatcccg caaagttctc ttccgcgtag gccgatatgg ccaggggcca
    47941 cagggaagag ccggcctcgg gcaccagggc cgagaccagc agaaagacct cttccccgga
    48001 aaccaggtgg gccactgctt tcctttcctc cttgggaatg aggaccaccg ttttctcctc
    48061 cggtgccggt tgtgcggaag ggtagccttt ttggccgaac cggtatcccg cctgcttgat
    48121 gaggcggcgg tactttttgt cttccaccag gtcctcgagg gtgcggaagg cccgaaagct
    48181 gacctccgtc ggccgtttgc tcagtcggtc gatcaacgcc gcaaccagct tttccctccc
    48241 ggtcagcatt tccctcatgg attggagaca gctatggaga gtggcccccc gggggttctg
    48301 gacggagagc ctgtcccaga tcatttccag ttcccggttt tcctcgggag ccaattccaa
    48361 ttcgctcttc tttgtgcttt tcatgtccgg tttcttggcc atcgctttgt tgtggacctc
    48421 ccccctcttc gcccccccat ttccccccga tggattccgg cagagctcca ataagaaaag
    48481 ccccttcacc acaccaaggc gtgattccgg ggcttgggtt cttcaaggat cgcgcggacc
    48541 tattcttcgc catcttcctc gcgaggctcg atgatcagag aatccgcaac cagctcccag
    48601 agatacttca cctccacatc catgaagtcg tattccttgc gaaggctctt catgatctgg
    48661 tcacggcagt tgtggcaggg cgccaccagc agtttggcac ccgtgtcttt aatctgtttt
    48721 gctttggtgc ggccgtagaa gatgcgctcc tccacgtacg ggctggccca cgccccgccc
    48781 cctgctccgc agcagaagtt gttggcacgg ttgggataca tgtccacgaa gttctcgcag
    48841 cactgctgag tgacccatcg gggttcctca tagtacccgt gaccgaatgc ccgctcgctc
    48901 ttgcgggcgt agttgcaggg gtcgtgatag gtggtcagtt cgtgatggac cgatttatcg
    48961 atcttgattc gtccttcctc gatatacatc ttgagcaagt catgcacgct cacgaacttc
    49021 acgccctcgt cggcatacca tttttgcaga ccaagcctgg tcgcatagta ggcgtggcct
    49081 cactcgggta agacaaggta ctcgcaaccg agctccttgt aattcttgac gatgcgaccc
    49141 acgatttcct tcatggcgtt gtcgtcgccc gtgaaaagcc cccagttgac cccttcccag
    49201 ttttccgaag tggtcgtcca ggactcgttc gccgcgtaca ggatccgcca ccagaacatc
    49261 atgtcctcgg gctcggcgaa aggttccttg gaattgatgg tgaagaggta cttggctccc
    49321 ttcttgtcga tgggaacctc gaacccgggg agctcttcgg ccagttcggc gcccaagtcc
    49381 tgcaacaaga acacgaaatc atccttgggg attcccacgt tgtttccggt cttgaggcac
    49441 atctcgacgc ccttgtgcaa cactcccgga accttgtccc ttgccctcat gcccctggcg
    49501 gaccgcagca gcgtcagaag gtcgatcttc atgggacaag catgttcgca tcgcccacaa
    49561 agggtgcaca cccacggaaa cctcgaatcg atcagttcct gatccattcc caatacggcc
    49621 agccgtaccg ctttacgcac gtccaggcca tccacgccgg tgaccgggca gcctccggca
    49681 caagtgccgc aggtgaggca cagctccgca tactggctcg ccagcttgcg cctctcgcgt
    49741 ttcgtcaaca acaatggctc catgtttctc tcccctagaa ttgttcaaaa ccgaagacgg
    49801 acacctgacc ctataacata gagaggggtc gtttcaaagg gttttctcaa agtcccggca
    49861 acggggcttc attttgcaca ttccccgatg cccgagcccc cggaggccct ccgccccggg
    49921 attccctgtc tctcccccgg ggggacatcc tcccatcgag gacgccggct acaggtcccg
    49981 cagtcccggc gaagaggcgg cacagcagcc tcgtgcggcc gctccctagt actcgttggg
    50041 cagtcccgag agtttttcca gggtgaacac cggtccgtcc ttgcacacat actccgttcc
    50101 gatgttgcat cggccgcaga ttccgattcc gcacttcatg cgcatctcga ggctcgtgat
    50161 gattcgatcc ggcggaaatc caaggtccgt cagcaccggc agggtgaatt tgatcatgat
    50221 gggcggcccg cacacgatgg cgtaagcatt ctcggaactg ggggctttct gcttggtcac
    50281 ggccgggacg aagcccacgt tgtatttcca ggtaggatcg ttggtggaat ccaccgtaat
    50341 gtgcatattg atgtccttgc gccgttccca ttcggccagt tcttccttgt agagcagcag
    50401 cccgggagac cgggcgccgt acaccaccga aatgtctccg aaccgggcgc ggttctcggg
    50461 atggagcatg tagacgatgg aagatcgcag agtcgtgaag gcaaatccgc cgccgacgat
    50521 gacgacgttt ttcccctcca gaatttccca gggataccag ttccccaagg gtccgcgcac
    50581 ccccatgact tcacccggac tcatgttgtg cagatggtgg gtcaccttcc ccaccttgtt
    50641 caccgtaaat ttgatgaacc ccttttccgt gggagaggtg gcgatcccga tggggatttc
    50701 ccccagcccc gcaacggaga gttccgcaaa ctggccgggc atgtactgga accgggcctc
    50761 gtcctcgggc ctcaggaaga ccaactcgaa ggttttcaga ttgcgatctt ccgtttccgt
    50821 caccacggag cggatacgta cgggataggg cagatatgga ttgttcacct tgattcctcc
    50881 gtcaaaaccg gcgcctttaa gcggggcagg tgcaactggt ggacatgagt cgagccacgt
    50941 ggcggatgtc tatgttcacg ggacagtgtt tgacgcatcg gccgcatccc acacaggcaa
    51001 tcccgtcctt gtatttttcc ggatagtatt tgagcttgtg catgaagcgc tggcggaccc
    51061 gctggagctt ttgggcgcgc gggttgtgcc cggacccgtg atgggtaaag aggggaaaca
    51121 tgcaggaatc ccagattcgg attcggtctc cccgttcctt gtagacctca tcctgaatgt
    51181 caaagcacca gcaagtggga cagagaaaag tgcaggtgcc gcaattgatg caggagaact
    51241 ggacctcgtc ccaaaagggg gcgttgaaca gctcgcttac ggtcttggac ttgagatcgt
    51301 ccacattgaa ctgtttgccc agttgaccct cggcctttcg ttgcagccgg gccgactcct
    51361 cggtcaggtc ggaaggcacc gcatcccctt ccatggaacc caaaagggtg ccgccttcct
    51421 tgctcacggc ccgggccacg tagccggtgc ccacatccac cagcaggatg tccagcccgg
    51481 tctcgtcgaa gggacccgtt ccggcggtgg tgcaaaagca ggattcacaa ggctcgttgc
    51541 aggccaggcc cactagcacg gcggctttcc ttctattccg ccaccatggg tcctggaagg
    51601 cttcggtgac atagttcagt tccaccagct gaaaggcccg agcgtcgcag ggccggattc
    51661 ccaggatcag cgtcgccgga tattccttgg ccgcctcctt gacgattccc gcctccggat
    51721 cgtccttggc cagggaatac tcgaacatcc gttcggaatg cggatgaagc aaccctttgg
    51781 gagacagtcg cgtgttccgg aagctcaaat cggcgtcccc cgcctccttc aatacctcga
    51841 aatcatgata catgccgccg aataccggac cgtatacccg gtaccggctc atgagccgat
    51901 ccaggatcct ggtgaatttt tccttgctga tatagcgttc cgccatggat gtattccttc
    51961 ttcctcaagt gtcgggaaac cgcaaaggat gggtggcgca ggtccttctt cacaagatca
    52021 tttgatgaaa gcctcggggt cgccgggtcg ataggtatcc agcgggggcc gctcgtcgac
    52081 actcaatccg gtctcgtagc cgtacaattc cctgatgtcc ttctcgagct tcttggtgaa
    52141 cttacgaacc ttgatcccca ccgggcatgc ccgttcacag gagccgcaat cggtgcagcg
    52201 gcctcccaag tggtaggccc tcagaaaatg aaaggtcagc gtgtcggtct tatcgaggct
    52261 tttccccacc cattggggcc gggattcgtc cacgaagcag gtcgggcagt agcaggccgg
    52321 gcaggcgttg cggcacgcat aacagcgaat gcagggctcg accagcttgc tgaagtactg
    52381 ccaccggtcc gccggaagga gggcctcgat ctcccggatg tccacatagc gatccacatt
    52441 ttgctgctct tccaccaggt cccccacgag ttcatcatag agaaccgggt ttcggtggat
    52501 gcagatggaa cagttttcct ggagaaattc cttccgatcc agggtcacct cgaattcgcg
    52561 cccggaaatc ttcaccgtgc cgtcggactc ctcggcgaac gtgggctcct tgcccttgag
    52621 ttccccggca atttttcgcc ggtccagcat tccgtgacag ggtgctccca ggatgtagag
    52681 ctgttcccgt ttgatctgga attccagtaa atgaaccacg atgtttcggg tatcacaccc
    52741 tttggccacc acggccaccc ggtccgtcct cttggcgagg tagttggcca ggtttacccc
    52801 gcaattgccg tcccagtaga gctgatccgc atccttgggg tccttgacca ggaagggctc
    52861 gttgaccagg ggaatggttc ccttgcgaaa cccgatgacc acgtccaccc gcttttccgt
    52921 cagcagtcgc ttggccgctt ctcgaatcct ctcggtatat gctcgcatct agaccacctc
    52981 agcttgcttt ttgatgaaat gttgggccgg ccccgccgcc ctcaccgcat cgacgactcc
    53041 cctggccact tctgcaaatt tggtggcctc ggcggaagag atccaggaga aatgcagtcg
    53101 attgggttcg atgattcccg tgtattcgag gaagttcttg agcagggcaa attttcgccg
    53161 tgcataaagg tttcccttga tgtagtgaca atcccccggg tgacatcccg aaacccacac
    53221 tccgtccgct ccgtgacgca gggtggccag aatgaatttc ggattgaccc gtcccgtaca
    53281 gggcaatcga atgattcgaa tgttggccgg atactgaaga cgactgatcc ccgctagatc
    53341 cgctccaccg tacgaacacc agttgcacaa gaaagcgaca attttcggtt cccactcgga
    53401 catgatcaca ttctcctcta tgcaagctcg agaccggaag aggcgggctt ccggggcgca
    53461 tgccccgaaa acccgggaat cctccgcctt ttccggcacg attccatcag atgctgtcga
    53521 tgaccctgaa gatttgtgca tcgtcgaacc cagccagccg gatggccccc gagcggcagg
    53581 aagccacgca cagaccgcat cccttgcaca gggccgcatt gatctcggct ttgcccttgg
    53641 cgttgaactg gggggcggag tacgggcaga tgctcacaca ggtgccgcaa ctgctgcaga
    53701 agaactggtt ggttccggcc accgtgccgc tgaactggat ggttttcttg gcaagaatgg
    53761 tgcacgcccg ggaggcggct gcctgtgcct gggcgatgct ctcgtcgatg ggcttgggat
    53821 aatgggccaa gccgcacaca aagacgccgt ccgtggcgaa atccacgggg cgcagcttgg
    53881 cgtgggcctc cacaaaaaac ccttcatcgt tcacagggac cttgaagtac tgacccagca
    53941 cttcgttgtc gtcggacacg atggcgttgg ccagagtgac caggtccgga gtgaagacca
    54001 ccgggcgttg cagtacgtgg tcgatcaccg tcacccggag ctttccgtcc gccacttcca
    54061 cccggggttt gttctccagg gaataccgga cgaacacaat ccccgagcgt cgtgcttcct
    54121 ggtagagaag ctcccgctct ccgtaggtcc tcaggtcacg atacaggatg tagatgtcgc
    54181 agtcgggatt gatcttcttg agttcgaggg cgctttccac gctgtgggag caacacaccc
    54241 ggctgcagtg gggacggccg ggttcccggg accccacgca ctggatgaag acggcgcttt
    54301 gcacctcttt gagcccggga tcccgggcaa tgaaccgccg gtccaggtcc tggtgcacca
    54361 ggacccgggg gtcctgccca tagaggtact cgttgggggt ccatcccttg cctcccttgg
    54421 caaccacggc gatgccgtgt tcgatgacct ccgagccgct cgcactttcc acggtggtcc
    54481 tgaaattgcc cacgaaccct tccacctggg tgatgtcggc ggaaagatgc acggtaatgc
    54541 gggggtgttc cgtcatggcg gccgccaggt ccttcacgta gggctggatc agctccccct
    54601 tccaggtctt gtacaggctc agggcctggc ctcccaggcg atccgtcttt tccaccaggt
    54661 gcaccgggta cccctggtcc gcgatggcct tggccgcggc cattcccgcc actccgcccc
    54721 cgatgaccag ggcactctgg gtcacgctca gctcgggctc ctggaggggc tccaggaggg
    54781 ctactttggc cacggccatg cgcacgaggt ctttggcctt ttccgttgcc atctcggggt
    54841 tcgaggcatg cacccaggaa tcctggtttc gaatgttggc catctcgaac aagtacttgt
    54901 tcagcccggc ctcggccatg gtctcctgga aaagcggctc gtgggttcgc ggcgtacagg
    54961 cggccaccac gatccggttg agattgtgtt cccgaatgac cttcttcagg accacctggg
    55021 tgtcctggga acaggtgtag aggttgtccg cgacgtattc cacgtaggga agggtcgcgg
    55081 catagtcgcg cacggcgggc acgtccacca cgccgctgat attgatcccg caatggcaga
    55141 caaaaacccc gacccggggc cgctccccca ccacgtcaat ctggggcggc gtctcctttt
    55201 cccgaaccag ggtcccccgg gccgatgcca ggaaggcccc ggccgccgag gcggcggcac
    55261 tggcttccac caccgactga ggaatgtcct tgggggactg gaaggctcca cagacgaata
    55321 cgcccggacg actgctcacc acggggtcaa aggaggtggt ctcggaaaac cgattcgtgt
    55381 tgagggcgat gcccagctgg tcggcgagtt cccggatagg ctgggtggtt tcgagcccca
    55441 ccgaaagcac caccatgtcg aaggtttcgc gcttcatgct cccctgttcc gtggcatagg
    55501 tgatctcgag gtcgtcggtt cccggcacgg gctccacgct gtggacccgg gacctctgga
    55561 aacggactcc gtgttcctcc ttggcacggt tgaagtaccg ctcgaaatcc ttcccgtggg
    55621 ttcgcatatc catgaaaaag atggtcgtat cgaggtttcc gcccgagtgt tccttcgcga
    55681 tcaccgcctc cttgatggca tacatgcagc agacggccga acaataggga tgatcgcagt
    55741 ggtggatgtc acgggaaccc acgcactgga gccaggcgac cttgcgcggc tcccggtgat
    55801 cggagggccg caccaggtgt cccatggtgg ggccggaagc cgagagcagc cgctcgaact
    55861 ccatggaggt caccacgttg ggcagcttcg agtacgcgta ggtatccagg gtccgggggt
    55921 caaagggctt gaagcccgga gccgcgatga tggcccccac atccagggtg acgagttcct
    55981 ccgtcatgcc gtgatccacg gcttccgcct tgcatgcgga tacgcactgg agacactcgc
    56041 agcaggccat gcaactgaga cacttggaag cctcggattt ggcctgttct tcggtaagtc
    56101 cccgttcgac ttcgtcaaac ccaaccttcc tccgggccat gtccaagacg ggcatgtgtt
    56161 cccggggccg ggtctcgatc cctttgggaa tgggcaggaa attctcctgg gagacttcgg
    56221 ggccgtcccg cccctccctc atgtcctctc cccgcaggta acgggagatg gagatggcgg
    56281 cctcccgccc ctgggctacg gcgccgatgg cgatccacgg accggattgg gcgtctcctc
    56341 cggcaaagat gcccggaatg ttggtggcaa acgtgacggg gtccgtctcg atggttccct
    56401 ttcggcccag ggaaatccca ttggagctca gaaagaggga atccggggcc tggccgatgg
    56461 ccggaaccac gtactgggca tccacgacga attcgctccc gggaaccgga acgggtctgc
    56521 gtctgcccga ctggtccggt tctcccagtt ccatgcggat gcaccgaatt cccgcgaccc
    56581 ggccgtcccg ggtgatcact tcctggggag ccgtaagata ttggatttgg accccttcgg
    56641 ccagggcgtc ttcgatctcg ttgtcccggg cgggcatttc ccggcgacta cgccggtaca
    56701 ggatggtgac ctcgtcggcc cccagcctca gggcacaccg ggcggcatcg atggccacat
    56761 cgccgcctcc cacgacggca acccgcccct ggacccgggt caactccttg aggttcactt
    56821 ggcgcaggaa atccaccccg tggatcaccc ccggggcatc ctctccggga actcccagct
    56881 tgaggctcgc gtgagctccg atggccagat agacggcctt gtacccgtcg gcaaacaggc
    56941 cgtcgagggt gatgtctctc cccaggaccg tattgtaccg gatttccact cccagccggg
    57001 tgatggcccg gacttccttt tccagcaccg aggggggcag acgataatcg gggattccca
    57061 cccggagcat tccccccgcc acggggaggg cttcgaacac ggtcacgccg tagccgtccc
    57121 gggccagaaa atgggccgcg gtcaatcccg ccggaccggc cccgatgacg gccacctttt
    57181 catcccgcct ggaaatctgc gggacgggaa gctccgtcac gtcgacctgg tcggccacaa
    57241 aacgcttgat ggcgcagatg gaaacgggct catccacctg accgcgacga cacgaggatt
    57301 cgcaaggatg agggcagatg cgtcccagga cccccggcag gggaagagtg cgcagaatga
    57361 cttccatggc ctcgggatat cggccctccg ccgtcaaggc gacgtaggcg tgggcgttca
    57421 cgtggttggg gcaagcctgg gtgcagggag accggtcgta cttgtcgatg gcgaaggccc
    57481 cgggaatggc ctgggcgtat cggcgaaacg tggcctttcg ccgggccatt ccctgttcga
    57541 acccgttctc caggtttacc gggcaaacct tggcgcaatc cccgcagccg gtgcattttt
    57601 ccggctggat gtagcggggt ttcttgagga ccttgacccg gaaattcccg ggttctccgg
    57661 agacttcccg gatctccgac aaggtcagaa gctcgatatt taggtgccgg ccgacctcga
    57721 ccagcttcgg ggagataatt cacatggcac agtcgttggt ggggaaagtc ttgtccaact
    57781 gggccatggc tcccccgatg gaggattcct tttccaccag gtagacatag tagccggaat
    57841 ttgccaggtc gagggaggat tgcatcccgg caatcccgcc tccaaccacc atcactgcgc
    57901 ccgtcattgg cttggaagtc ccgtttccct tcatgaacct ttactccgta gacgatccgt
    57961 ccataagatt tgcgaaaacc ctgcacgcgt atcccgggcc gattccgcaa ccgattccac
    58021 cccgaacaag atgcacaagt gcacaaaaga aattttgcac atggactata ccatagaatg
    58081 gacatcctgg tcagtacctt ttttacgaat tttttttgat gtgatcgaac ttccaatccc
    58141 c
//
LOCUS       DAOOVC010000010        50878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_416_length_50878_cov_5.808276, whole genome shotgun sequence.
ACCESSION   DAOOVC010000010 DAOOVC010000000
VERSION     DAOOVC010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 50878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50878
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_50878_cov_5.808276"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PLM79_02620"
     CDS             complement(<1..748)
                     /locus_tag="PLM79_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85225.1"
                     /translation="MIPKCRFRWFSSAVAAALVSLACLPVVDALMTDRVPPDQLYPSL
                     PKTAVTRRFAWEDVNTWRLLRVEGDRQIFDARFPALRFTLEVVIDPLRLQKAVESYGI
                     PESWMRYSFRTEEERRRKEKEQTLRCAARGIARDPSPNSLEPDYYWIVERSREDVREA
                     AARLRDVGLQAGYDDLRRFVELSASFVQSLEYRLPPDTRRGRDGVLIRTFGLTMPLET
                     LYNGHGDCDTKSVLFASLLVNLADTRLILLR"
     gene            complement(745..1287)
                     /locus_tag="PLM79_02625"
     CDS             complement(745..1287)
                     /locus_tag="PLM79_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOV85226.1"
                     /translation="MDWNAILVGILELLLAFGLGFLTVWLAFRSFARFTRHLDEMEEL
                     KRNNASMGILLASIVLSTAFVVRAALYPSVSTWQTLVHRGFSGGDLLFCVGISLFSFV
                     FSSGIALAGIWSALRIFLRLTREVDELFEIGRNNLAVALVLGVLIVVMGLFLSHGVQS
                     LLSAMVPMPSFERIQVMGMP"
     gene            2222..5416
                     /locus_tag="PLM79_02630"
     CDS             2222..5416
                     /locus_tag="PLM79_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85227.1"
                     /translation="MTLIASIHGSRTGISASWLLVAFILAGLVGNHFPFSILNAHFVF
                     GSIFSMLALQVLGWGRGVLAGALIAAYTYVTWNHPWAFVTMTAEAAAVGWLFSRRRIT
                     LVAADAIYWLFLGIPLGYLCFRLFSDLPASNALFLVTKQGINGIANTLAARLILTGLS
                     LRLNTVRLSFREAVSNLLIFFVLCPSLILPALGGKADLDETDRQIRSSLVRDSRQAAG
                     NLDMWVEDRRRAVVHLAELAGTRSWKELQPRLEQTLASDANFLHIALIDREATVVAHS
                     PISDELGRSNIGKSCADCPYLARLKQDLKPMLAEVAISRFGRPDPIAIMAAPVVLGGS
                     YEGAVGGILNLDRIRIILETSSQGSDTLYTLLDRNGAVIMTNRPDQKVTTPFSRGRGT
                     LHRLEEGMFRWVPELPPGTSTIELWGQSAYAMESTVGDLAEWRLILEQPVAPFQRRLY
                     RSYTGGFFLVFVILVASLVPAQFLSRMVVETTEKLSFLTRDLPAKLASGDTVTWPRSA
                     ILETNRLVANFKEMAESLAARFAESRRMNEWLEGRVEERTEELRNSEEFLSNIVENIP
                     AMVFIKDAADLRFLMFNRAGEELLGYSREELLGKSDYDFFPREEADFFTAKDREVLST
                     GRLLEIPKETIQTRHRGERILRTKKIPVLDARGKARYLLGISDDITDQVRLQDQLFQA
                     QKMESVGRLAGGVAHDFNNMLGLILGHAEMALDEVDPAQTIHHNLQEILTAAHRSADL
                     TRQLLAFARKQAVSPRKLDLNETVGGMLKMLRRLMGEDIDLVWKPGLDLWPVRVDPSQ
                     IDQVLANLCVNARDAVVGAGKVTIETGNATFDEAYCREHAGFFPGRYVRLAVSDNGCG
                     MEKEVLEHLFEPFFTTKELGKGTGLGLATVYGIVRQNSGFINVYSEPGQGTTFKIYLP
                     RCDIEAPEEPPSPGEKRDLRGTETVLLVEDEEAILLLAKTILERYGYQVFTAPNPAAA
                     IHVAETLSDPIHLLITDVIMPGMNGKDLFERLNLLRPGLKCIFMSGYTANVIAHHGVL
                     DEGIHFLQKPFSVEALAEKVREVLDTSGSKVSPS"
     gene            5987..8479
                     /locus_tag="PLM79_02635"
     CDS             5987..8479
                     /locus_tag="PLM79_02635"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOV85228.1"
                     /translation="MKIQPKWRHWKIRTRAGVATLVGFLVVGSLSIFLVHKQILRQAV
                     REAEIKSQLMLDRNLAIHSYFTHQLKPRLLPITDASQPREYFDPVWMSSTYAVREIQK
                     YFSQLGNDTYYYRECAVNARSPENEADEIERDFIRRTGLDPALRYESLMRRIDGKPYL
                     QVLRRGEVMEEACLRCHTTPDRAPEGLVKEYGAERSFHRSLGEVVSAISIRIPLAAAF
                     AQARDAAVRLSLLLVGLLSVLSVIQFWSVERLIATPLARLRDQARRISSGKDEPGGRI
                     PRPAGRELIELTDAFNHMSSRLRQHHDELEGIIRERTARLLAGEEFQRAVVECSPVAL
                     ISTDPGGNVLTWNASAEQIFGWSAQEVAGRPLPIVPEGKEEAFGELCKRLLAGRTFSR
                     MEVKARKKPGELLDAAFSASPIRDAGGSVIGMMYAVEDITDRKRAEKDKTALETQLRQ
                     TAKMEAVGRLAGGIAHNFNNILGIVLGFADLAVLSLENDKTEARAHIAKIKDAAARAS
                     LLVRQLVTFSKKQAPQPQVVDVNLAVSEMEKLLRGIIGEDIELISILDPQLFRVSTNP
                     GSIDQILMDLALNARDAMPVGGRLTIETRNVFLNGTDSHGPLLARPGPYVMIRVTDTG
                     TGVKESIRDRVFEPFFTTKEVGDGVGLGLSCVYGIVEQSGGYVRMDSEEGRGTTFEVF
                     LPAVAQEPEGKTRESLPVLPSVQGSETILVVEDDERLRGTVCQVLEKAGYRVLQAENG
                     TAAIEIVTEYPGPIHLLLTDVVMPGIGGPELASRLRGLKSETKVLYMSGYTDHPSGNR
                     EASGSPAAFLPKPFNSSQLTQRVREELEKDSS"
     gene            8514..11801
                     /locus_tag="PLM79_02640"
     CDS             8514..11801
                     /locus_tag="PLM79_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85229.1"
                     /translation="MKTPRKRAQRFPKDDAQPEGRGSAPRSPGPEKDSLDCLISFSRE
                     LSSARSLAELMVAVRNAVRQLTGADGATFVLREGDLCHYADEDAVGPLWMGSRFPMDA
                     CISGWVMKEKKPAVIEDIYADERIPQDLYPPTFVKSLVMMPIRSADPIGAIGVYWAER
                     HRPGDGEVKIIQALAESTAVALGNLQLLEELARSAGEVRDFLETLVKTSESLILVCDP
                     GGRITLFNRACEELSGYAREEVLGKTVLELFQGASRPSPGGRGFVHPFDPCLRLPQLH
                     SWKTRSGEERLIEWRCSFLPHPEDDEPRSHSFEPLPSGSERFFQLLFEEAPLPYQSLD
                     ETGCFLHVNRAWLERLGYALEEVLGRNFAEFLAPQSLDRFKENFPRFKALGEVHGVEF
                     DMVRKDGSVMQVSFNGKVARDIEGNFIRTHCIFEDITERKRAQEEKRRQQDVLSRVFR
                     ASPYIMMLVDREGRVLKINRAGLEFSGRSEGELLGLSGGQVLNCIHAHTAPVCNRSPA
                     CGDCLIRNRMLHSFQTGESTFDTECSLRIRKGATEGVMDMLLSTTLVKNGGTDTVLLT
                     IADITERKRNERRLREYEKVVESLDEMIAVVDRDYRYLIANRAFMKNRAPGTGEVLGR
                     PIPEILGEEMFEEIVRERLDECFRGKVVRYEMQCPHPGVGVRDLSITYHPVEGPGGVD
                     RAACIMRDVTEQRRGEEEKKKLRDQLYQTQKLEAIGTLAGGIAHDFNNILGIILGFAE
                     IALGEHAERGLASGALDQIVKATHRARELVKQILTFSRRTQQDRRLVQVSSLVKEALK
                     MLRASLPTTIEIRSDLQGQGMVNADPVQIHQIIMNLCTNSYHAMRPHGGVLTVGLADV
                     EFGERTPHPDLPNGAYMRLTVRDTGSGIDPAILDRIFDPFFTTKDLAEGTGMGLSVVH
                     GIVKSYGGGVTVESEPGKGSTFTVYLPRVQAQPGKHLEKLRPALPGGKERILFVDDET
                     LLAELSAKMLSALGYQVVTEASSPAALELFQREPHRFDLVITDLTMPQMTGDELARRM
                     MEIRRDVPVILCTGYGEMITREQAQTMGIRELLLKPLSRDDLALTVRNVLDGIAAGSQ
                     RRGDLSD"
     gene            12000..12977
                     /locus_tag="PLM79_02645"
     CDS             12000..12977
                     /locus_tag="PLM79_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85230.1"
                     /translation="MENALSRNVLIVDDEEEYRELIRSYLSKRGFSCEVASNGEDALA
                     LLQKKPFDVLITDIRMKGMDGIRLMTEAHKGQPDLDVIIITGYAPAYSYTEIVHAGAA
                     DFIAKPFEIEELHAKLDRIRRERRAFRQLQLVLEQSIRALSLAMEFKDPYTAGHQRRV
                     AALAEAIALELGLSRGVVDAVRMGGLVHDIGKISVPSEILAKPARLQPVEFQLIRQHT
                     EAGFEILKNVDFEWPIAEIAFQHHERMDGSGYPRGLTGDKTLVEARIIAVSDVVEAMS
                     SHRPYRAALGIDAALEEISRGRGTWFDPEVVDVCVRLFREKHFEFNAGG"
     gene            13068..15242
                     /locus_tag="PLM79_02650"
     CDS             13068..15242
                     /locus_tag="PLM79_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85231.1"
                     /translation="MKEPAETRRILVVDDEEEYRVLATTFLERSGYTCASASDADEAL
                     RKLDERPFHLVVSDIRMKGKDGLRLMAEARGTHPHLDFIIMTGYTEDYTYSDIIKAGA
                     TDFIHKPFDMHQLLAKLQRIEREKSTLARLQEAYETLSWEAEVNRSFAELSRALISAR
                     PFEDISSLVLEHAKALTGSPLGYVSHVDRETGHLTVSTMAAGTWELCRAEDKDAAFRD
                     FHGLGARVLENGKSLRINDFQRELPSGRLPAGHIPIHRFLSAVATVEDRMVGQVSLAN
                     SSRDYSEKDLVVIERLADVFAVAVQRRQMEDSLRKSHGETEQLLASIPSLLIELSEED
                     RVVRWNPAARRVLGKSLEEVFGLPLEEVWMPVDWKRVRAGIAAARSTGKSQRLDDVRF
                     LRSDGKEGFLSLSISCVQDHSAGKTGLILLGSDVTERKILEGQLAQAQKLESIGQLAA
                     GIAHEINTPTQYVGDNVRFLQDAFKDLERLLDKYEELRASVRDGPPTPAVISAVEEAV
                     EQTDLDYLKTEIPKAIEQSLEGIRRVSKIVRAMREFSHPGLNEKTALDINRAIESTLT
                     VTRNEWKYVAEVVTDFDPNLPLVPVLPGEFNQVILNMIINAAHAIGAVVGDGSGAKGT
                     ITLRTRKRDDSVEISIGDTGTGIPEEIRSKIFDPFFTTKEVGKGTGQGLAISHSVIVE
                     KHGGKISFESQVGKGTTFFICLPTQQDLQSERRHEKEHSFCG"
     gene            15214..16428
                     /locus_tag="PLM79_02655"
     CDS             15214..16428
                     /locus_tag="PLM79_02655"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85232.1"
                     /translation="MKKSILFVDDEPKVLEALQRSLRPMRREWDMTFADGGAKALEVL
                     AAKPVDVIVTDMRMPGMDGCRLLAEVRTRHPRVVRIILSGHSDRETILKSVGPAHQYL
                     PKPCDTETLKTTIARACALRALLTDESLVQVVSRVESLPSLPSLYSEVVRELESPYGS
                     IQKVEQIISRDLGMTAKILHMANSAFFGLPQHVSSPARAVRLLGLETVKALVLTVQIF
                     SQFPEARLPGFSIEDLWHHSMTTGVFAKKIAKEEKQDPHALDDSFMAGLLHDIGKLVV
                     LANFPDRYGEILHRVRETGASMWESEQAVLGSTHAQVGAYLMGLWGLPDSIVEAIAYH
                     HCPQDCLGRSFSSLTAVHAANAFAAHAPRREEMEGIPHLRMDYLEALQLAHRVPRWRE
                     ICGELTREEDAP"
     gene            16425..17579
                     /locus_tag="PLM79_02660"
     CDS             16425..17579
                     /locus_tag="PLM79_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85233.1"
                     /translation="MTEKILLVDDDPNILAALRRQLRKVFLIDTAPGPREGLEAVRES
                     GPYAVVVSDLRMPGMDGIQFLAKVRTLAPDTVRMMLTGNADLHAAMEAVNEGNVFRFL
                     TKPCPPEVLVKGLWAGIEQYRLILAERELLENTLRGAVKALMEILSLVNPEAFGRASR
                     VTRYAVEVGRKMKVAELWQLETAAALSQIGCIILPEQALRKVYRGESLTQQEERLFQK
                     HPTVAAELLAKIPRMQKIAEIIAYQAKEYNGGGLPRDPRRGDEIPLGSRILKAVLDLD
                     AREARGEPPERALMEMKARRGMYDPAVLGAIEAWIGAETRLVEQTLESPEQLSEGMIL
                     AEDVCLSDGRLLVARGYPVNRTMMERLRNFARKPGLKAPLKVLVRNPGGI"
     gene            17582..18172
                     /locus_tag="PLM79_02665"
     CDS             17582..18172
                     /locus_tag="PLM79_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85234.1"
                     /translation="MSRTILLVDDEPLVTEALKRALRKEPYLVLNARSAAEALRILEK
                     HPVDVVVSDERMPGMTGSEFLAVVCRKYPETMRIILTGHADLESALRAINEGHVYRFL
                     RKPCNEAELIHTLRQAVQQKDLASGSRRLLHAFRKQQSELNALEKEHPGIRTVKRDER
                     GAIVLEEEEEDYDLDTLIEEIEREVRQDETRPLPPR"
     gene            18294..19406
                     /locus_tag="PLM79_02670"
     CDS             18294..19406
                     /locus_tag="PLM79_02670"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV85235.1"
                     /translation="MIPQLFVAFLTGLTAGGIGCLAVQSGLLAGSLARRFEGCAEVEG
                     VVGPAETGAAAEPRFAVTIALFLLAKLAAYTGTGFLLGAFGSLVRITPLSRAALTILI
                     GTFLVGNGLRMLGVHPVFRWFVLEPPSSLRRFIRRASGRTVSPVAPVFLGTLTVLLPC
                     GVSQAMMAAAVGTGDPFLGAALLFAFTLGTIPIFFSVAYFATRLSAAVQAHFNRFVAL
                     ILLGFGGVSVYYGLNLAGIPFGLSYSDHGPAEQSGFSNPPGPSETGTNGKGEYVISVS
                     NGGYEPKVLHLPANRPVTLIWVTRDGTSCTRSVVLPGLKYHAILPSSGRSPLTIPPQK
                     LGTRLPYCCSTGRFTGLLVFDGKEEGTDPSPEGTRP"
     gene            19460..24301
                     /locus_tag="PLM79_02675"
     CDS             19460..24301
                     /locus_tag="PLM79_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOV85236.1"
                     /translation="MKLRFRVPDMVCPDCTMHLESLEDTLPGVKRIRASYRRLSMEVE
                     FDERRLDAREIIAAANAIGYHPEHPLVGKPEASTPTSGLLLPGEATKSTRRIVLPVKG
                     MACSTCAGGIERQLLRLPGVVEARVDFAGERVAVTFDPAGIDLDGILTRIRRMGYRVP
                     TGRAVLSLDGLREQDDVGKLEGLLAVQDGVLAVRVNHAIHRAGVEFIPGVTDAGELAR
                     AMRKGGFDRVRTRDFRELEDDEGGSHATEEKRGKRMMILGIAFTLPLVVYSMAQDFGL
                     VGFDHDLAVMLLAATVVQFVPGLQFYVGAYRSLEAGTANMDVLIALGSSVAYFSSLGV
                     VLGLVPGPNVYFETGASVIALVRLGRYLEARARSTASQALKLLADLGARTARIRRGEA
                     EIEIDVEDVDVGDLVVVRPGEKVPVDGIVESGRSSVDESMITGESMPVGKAPGDEVIG
                     ATLNQEGTFTFQATQVGKNTTLARIVRLVQDAQAARAPIQKLTDQIGAYFVPIVVAMA
                     MGTFLGWIYVANIEWSGAMINAVAVLVIACPCAVGLATPTAILVGTWKGAEHGILFKT
                     GEALERAGKVNVVVLDKTGTLTRGMPEVTDIVPAAGEDPDVLLRLAAGAERGSEHPLG
                     RAVVRAATDRGLDPAQPEDFLAVAGQGVRAVVDGRTVLVGSPRMMPREGIDLEELAGE
                     VTRLESAGKTVMAVAVLEDSSPRPMGLLAVADAVKPESRTAVAELQQMGIDVVMITGD
                     NRRAAQAVASQVGIERVLAEVLPGGKAEEIRQLQAAAPAPGLPRPVVAMVGDGINDAP
                     ALAQADVGIAVGTGTDVAVAAAGITLISGDLTGVGRAISLSRGTLQTLYENLIWAFFY
                     NIALVPIAAYGLLSPMIAAGAMAFSSILVVTNSLRLRGYKVRTFEAPKDLWRQCLDLT
                     PRILAPTATLGILIVLPMLTMAQGAEIRGVLPSTMSPVLMMVMAIANGLIAVSYWSIP
                     VFLLVFIHRRKDLPFSWVIVLFGTFILACGTTHFVHILGLWWEVDWWQALVDSLCAVV
                     SLATAIVIWPLLPKILAIPSPEQLRIVNRELRLERDALERTQAELRRAYADMERRVEE
                     RTAALARSNEALRLEIAERKRAAEDRERLMAAIQQAGEMVVITDLDGTIQYVNPAFET
                     VTGYSRREAVGRNPRMLKSGEHDAAFYRELWDTVSRGYVWRGRVVNRRKDGSLFTEDA
                     TISPVCDASGRIVNYVAVKRDITAEIRLQAQLLQAQKMESVGRLAGGVAHDFNNMLGI
                     IIGHAEMALDGVSPSQPIYRELREILSAAHRSADLTRQLLAFARKQAANPRVLDLNET
                     VEGMLKMLRRLIGEDIDLLWKPGSRLWPVRVDPSQIDQVLANLCVNSRDAITGVGKVT
                     IETGNVVFGEEYCREHGGYVPGEYVMLAVSDNGCGMDKKVMGQLFEPFFTTKEVGRGT
                     GLGLATVYGIVKQNSGFINVYSEPGQGTTFKIYLPRVESLPSLKQGPSSASRDLRGSE
                     TVLVVEDEESMLELAKAILEKHGYRVFAAGDPAKALELAQKHPGRIDLLITDVVMPGM
                     NGKELNERVAALSPGLKCIFMSGYTANVIAHHGVLDEGIHFLQKPFSMQAMAAKVREV
                     LDS"
     gene            24622..26292
                     /locus_tag="PLM79_02680"
     CDS             24622..26292
                     /locus_tag="PLM79_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV85237.1"
                     /translation="MSPSAFDYDQREAYSRSCSLMFHPARGAVVKETFGTRSIPFCID
                     LEESTAISFGGRLATISRFGFAGCQGVTGKIFKRGGTSRETVQKEIGSQLRAMDLDCV
                     FLLGDNFYDDGIGDVRGARFTTCFDDIYPTGERPVFMVLGNHDYNLHSHGDNPLLSSG
                     AKSPSERMTWALTQVQRTYYLLQARHTSKWTMPYRYYALFSRNLHFIILDSNTYPFDA
                     FQQQWLEDVCGYSLKRGKGNVLRALIMHHPVIARGKRAASLDDKNRYAATFGAPLEDS
                     RARRGLRTNSMSDYMYNSLVSLNTRNLLVFDFILCAHDHLTSSEIVRIGNREVLQVIS
                     GGGGGNLEDEKKVEKSDAIYDGQVDKPELEHLIGRLEHNEYCIIQHGYHVATVYPDKR
                     IRFQCMGKSDTRTAVYSFKPGERVGDHLRQTDGRALPPEIMTPRKERLDRYVATLKNA
                     IEQHTWEFSVFGGGETVEVGPGRSIKVPKNVKSMVDAIKQYERNPHNVEIDVEIQRIA
                     SLALSRGSAFRAADTTRFYREWSTKTVDDCIREASRAGTRRRGLIIAE"
     gene            26443..27159
                     /locus_tag="PLM79_02685"
     CDS             26443..27159
                     /locus_tag="PLM79_02685"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HOV85238.1"
                     /translation="MKALSVQQPFAFEILSGQKSIEVRTWDTLHRGDLLICSSGRPAY
                     SRGEMEELEDEYGCIFRYGTALCVVRVADVRPMSRGDEERALMDEIDPDSYSWVLEEV
                     RPVVPFPVKGRQGLFEVEDSLIVVSPFRYDQSVVVKTGTVAQDFGIDFSGWRGRVSDV
                     FPGEDGEPRVFVQWDSLSLEALPIGVIETCEREKVDWTGVLLRFSEIEPAKPRDAWED
                     VWDAVERIYEENAHLFPDQD"
     gene            complement(27390..28031)
                     /locus_tag="PLM79_02690"
     CDS             complement(27390..28031)
                     /locus_tag="PLM79_02690"
                     /inference="COORDINATES: protein motif:HMM:NF025595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HOV85239.1"
                     /translation="MSDAMPAEQDLEREKKRFEIVCALTLAVFAALLAITDLGASKYG
                     DDEIMGTNEKANIYAWFQSKSVKQSLVEGQRDLVKTLMEAGSIDPAHQSTLEQLLGRL
                     EQDIARYKKEKRELLLGSAKVGKENWVQDIDGELGKIVGAKEWEAKLSVLGQAGDKFD
                     LALLFLQVSLVIGAVSLVLQENRLRWLFYGTMSTLGILGLVFSVWAFFLASSA"
     gene            28274..28507
                     /locus_tag="PLM79_02695"
     CDS             28274..28507
                     /locus_tag="PLM79_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HOV85240.1"
                     /translation="MRVILKTLLLAILMSSLAGCANMSAREQRMLSGGAIGAGTGAAI
                     GAVTGGSPAVGAAVGGAAGVVGGAIVDELENRR"
     gene            28738..28935
                     /locus_tag="PLM79_02700"
     CDS             28738..28935
                     /locus_tag="PLM79_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85241.1"
                     /translation="MKIGGKIVYCLASLAFVVISGAAMGARAGTGMDRFDTYDLSSPQ
                     IERLPDGNLESPYPPYYRITP"
     gene            29996..30907
                     /locus_tag="PLM79_02705"
     CDS             29996..30907
                     /locus_tag="PLM79_02705"
                     /inference="COORDINATES: protein motif:HMM:NF039734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HOV85242.1"
                     /translation="MRRGILCFIVALTTLGFANPGVALAGGGTYDAHLYAITQWTTTC
                     SAGARDLWDDMGKAWYNEVTNSGFSFFGWCLWGHCDDYYNAARQWVDGAMSAAPFTDR
                     TLFPAWGQDTSYVDIADAAMICTHGGDDGGYWRGKMRNTDGNGDCYINANTELRVGDW
                     DLEFLHLSSCHSLDDNMIPNAYRVFSRAGSSRILHQLDGFHGCMWIGSSFIADYEDFA
                     DDAFDIPIGLSWMLNMYRTNVNGQWTQCPIAYAVGSSADDCINRLTGERYNNVYSDPS
                     AVNYYCYYYYPNCAPDCETPFGQDWSN"
     gene            30943..31893
                     /locus_tag="PLM79_02710"
     CDS             30943..31893
                     /locus_tag="PLM79_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85243.1"
                     /translation="MKILFMVAFAVVLVMPGAALAQTGGCEAYFALIEDNLPSMVGDD
                     AALTSLITVTANVWTDNVDPTKIMKSLGEREFSQKGLEKYRVGLMKSSSSKMKYNLER
                     GELRYVNQDRRFDSAKPGKAIDPSKGLEMARKLLSGNIGVPRSEILGKNTLSQVLQGG
                     IARPGTQDLVKRYDIESHFFFQRAINGIPVMNSGAFVAVSNKGQISQFQVRWPVMRVD
                     QSLLKAGVLSQKEVINNIYNRLVQDQPCDQTTSIKKLNMFIAYVPSVVTNPDKDKIPE
                     PARRVRYTPKLIVYFLPDADGEAGEVLEFDLFNKDGRPES"
     gene            32088..32285
                     /locus_tag="PLM79_02715"
     CDS             32088..32285
                     /locus_tag="PLM79_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HOV85244.1"
                     /translation="MPYVNIKITKEGATREQKALLIREVTRLLQEILGKDPQTTFVIL
                     EEIETDHWGIAGETVTVRRKR"
     gene            32580..33665
                     /locus_tag="PLM79_02720"
     CDS             32580..33665
                     /locus_tag="PLM79_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HOV85245.1"
                     /translation="MPGRCALSWAIILVLSLSGISGCGFPRLPEKKHEVQLQEFARSD
                     RRWTGVAVSQSGRVFVAYPRWSEDTPISVGELLLSGEVVPFPDPEWNRWNPGLSPANH
                     LVCAQSVVVDSEDFLWVLDPANPQFQGVVYGGAKLLRVDLSTHEVVRRILFDESVALP
                     ASYLNDVRIDNRHGYAYLTDSGTGALVVVELASGKAWRRLANHRSTQSENLSLTIGDR
                     PWLRGGKPPRVHADGIALDRAGDYVYYQALTGRTLYRIPASLLRDPNVSEEQTAKGVE
                     RVGVICAADGIEFGRDEKLYLTDIEGSGIRVYEPGKGKRILIRDKVLSWPDSIAVAPD
                     GSLVVSTSQIHLGDHPSGPYRLFRIKP"
     gene            33745..34137
                     /locus_tag="PLM79_02725"
     CDS             33745..34137
                     /locus_tag="PLM79_02725"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HOV85246.1"
                     /translation="MKDFSSLGVTREQCKDTGMAMVLLCLLGEYFTHRPDLLSVAVGL
                     LVLDMIFPVFFKPVAVIWIRLSHVLGGVSSKLLLTLVFFLLLTPMGLIRRALGKDPMR
                     RKQWKRGSSSVFHLRNHTFTAKDLETPY"
     gene            34164..34313
                     /locus_tag="PLM79_02730"
     CDS             34164..34313
                     /locus_tag="PLM79_02730"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HOV85247.1"
                     /translation="MDFLKDFWGYLRVRKKYWLLPVLVTLLLFGALIVFTGGSAIAPF
                     IYTLF"
     gene            34326..36185
                     /locus_tag="PLM79_02735"
     CDS             34326..36185
                     /locus_tag="PLM79_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HOV85248.1"
                     /translation="MRKAILGISAYYHDSAAALLVDGEIVAAAQEERFSRKKHDASFP
                     ANAVEYVLKEGGMGLGDLTAVSFYDKPYLKFERLLETYHGFAPQGLRSFLAAIPVWIK
                     EKLFLRDLLNKELSRFGKSRPRLLFPEHHLSHAASAFFPSPFQEAAILTIDGVGEWAT
                     TTIAHGKGNTVTIHFQLDFPHSLGLLYSAFTHYCGFKVNSGEYKLMGLAPYGNPDSLR
                     TRRWRQLIFEELVDLREDGSFLLNMDYFDYATGLSMCRDARWEELFEIPRRRMEGELV
                     REYADLALAIQMVTEEIVGRLARTARQVTGSPYLTMAGGVALNCVANGKLLRSGRFED
                     LWIQPAAGDAGGALGAAYAAWYIREGAERSVSRDGEPMKGAYLGPEYGLKDFRRTARK
                     HSAPYVEFPTLEEMVEAVADLLARGCVVGWFQGRMEYGPRALGNRSILGDARDLQMQK
                     NLNLKIKFREGFRPFAPSVMEEDLQEYFELDRPSPYMLLVAPVARRRRKPVPNGEAEL
                     EILERLYHPRSDIPAVTHVDYSARIQSVSRQTNETYWRLLRAFKEKTGCSVLVNTSFN
                     VRGEPIVCTPEDAYRCFMRTQMDYLAMGNLLFDKKHQAPIPEDGSWRLQYSLD"
     gene            36196..37443
                     /locus_tag="PLM79_02740"
     CDS             36196..37443
                     /locus_tag="PLM79_02740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOV85249.1"
                     /translation="MTRAKSLIKWVLNLGILLYMVFLILLFLSRGAGYDFQIGFLRVQ
                     LRRPGNPLMILAVLTLLRILLEMEKKNAVLFVFSLLFSLSLVEMGLRVLDLPIANQPT
                     LNKWKRPSPSLGWELIPFSKGKGHEGAYVEINSHGLRDVERSWEKPEGQYRILCVGDS
                     FTYGVGVDLKDTYPKQIENLLNSTGHSADVINSGVPGYDLYQVLTYLQSVGTRYDPDA
                     VLYFFYCNDLAGLKSADEVKTYYDALVNSEEDRESVYDFSGSYLINFTRNAYTFLWAK
                     FRFLYDADWIRSLHKRKKVFSREWIKVLVGDFDVEFLRKSLARMKDFTDEHGVRFMVV
                     FVPDAVQLHNPSWQEINRIVGQLCRSQGTPFVDVTPTFEEQPDAAALYLFPMDAHTSP
                     RGNALLAGRVRDAIRDLGWLSNP"
     gene            37497..37817
                     /locus_tag="PLM79_02745"
     CDS             37497..37817
                     /locus_tag="PLM79_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85250.1"
                     /translation="MGTIPILSRNQSCGDTNFRECGLFQATLRELPDFYMTDYSVWGL
                     LVDNPREVLQLLDEHGFRVIRKPGAMEVEIDGFTRVHEIVRLFSAAGIAFEMADLVDG
                     VYQG"
     gene            complement(37860..38033)
                     /locus_tag="PLM79_02750"
     CDS             complement(37860..38033)
                     /locus_tag="PLM79_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85251.1"
                     /translation="MLQNTAGLRIPEDPVAGRTAAPGNAVRLQEEEPERETDGLEDYY
                     EEEDPEDKPDSEI"
     gene            complement(38258..38965)
                     /locus_tag="PLM79_02755"
     CDS             complement(38258..38965)
                     /locus_tag="PLM79_02755"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV85252.1"
                     /translation="MKKALLFDIYGTMIDIRTDEHDPSVYEALSRHLAYLRVFVQPEE
                     LKNAFLQQVREALQRSKEAHPEVDVFRVFEDLLRHFGRKKPSEKTVLHVAVLFRSLTI
                     RTFGPFPGILEALPVLRKTYRMAIVSDAQWVFTEPEIRMLGLDRFFKVILLSSRFGFK
                     KPDPRLFHLALDRLGVSPKDSVYIGDNPQKDLVGAKTAGMSFLLFRGEGKEVNGFRPD
                     GVLRNYSELPDLLRKMQ"
     gene            complement(38943..39965)
                     /locus_tag="PLM79_02760"
     CDS             complement(38943..39965)
                     /locus_tag="PLM79_02760"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOV85253.1"
                     /translation="MTKALFLGILDNHDPLYEVLATRVCPQVRRPVFHVWRMSSSLVY
                     RYREEESRVTIIGKFFRLEDALGDRALRIKGEYENLLRVRNLGFDRFPHSVARPITRE
                     EGIGLALVEEFADGCDLDHYLKKAIYRGEHPPLKEALGQLARFLSLLHAKTAQESRVD
                     LGFVGAYFQKILHRLLAKGVVPESEAPSLWTLMDRWLNSDCLRRTENVVVHGDATPTN
                     FIFTEDGGIVAIDLERMKDTDRAFDLGMVCGELKHAFFWRTGNPFGAEPFIHHFLRSY
                     CDRLHHGQEGFVEITARIPFYMALTELRIARNLYLDRDYRRRLSREAFQCLKWGWKLH
                     EKGAPV"
     gene            complement(40221..40463)
                     /locus_tag="PLM79_02765"
     CDS             complement(40221..40463)
                     /locus_tag="PLM79_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85254.1"
                     /translation="MTEIERAQVQVLMERGKLRFVPVQGGHSYTWDGMEIFVPGDLSR
                     AEEHIAAFLRREKIRVHHRVLRSHLGFLHRRLHKDE"
     gene            complement(40549..41073)
                     /locus_tag="PLM79_02770"
     CDS             complement(40549..41073)
                     /locus_tag="PLM79_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85255.1"
                     /translation="MGFRKLFTVMLSFTLSLGMGAAVSRAQNVLEQYDETLRQLREDY
                     RQILKQDSGPARRHSKASPSSRSKHKGKSSSKSRKLSKSGSKGKKFGKSSGKSKSVDR
                     GKSKTVGKAKGPTASKSSKAVTGKTSRTQPSQVTRTGSGRSAGKSAGKSQKTETSPKK
                     TSAQKPETRKKAKR"
     gene            41641..43827
                     /locus_tag="PLM79_02775"
     CDS             41641..43827
                     /locus_tag="PLM79_02775"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOV85256.1"
                     /translation="MFGKRSLRFKLVFLGVLLSLAPLVLITANTFFQNKKIVTDAGAI
                     ISAQAYADLEHLVKNVHAIVETQNIVLQDSVKNCINVARKVLSDAGGIRLSGAEKVPW
                     KAVNQFTKQEVTVELPKMYVGDSWVGQNKDMKISTPMVDDVKSLVGGTCTLFQRMNDA
                     GDILRVATNVEGADKTRAIGTYIPAVNPDGKPNPVVAALTKGQTYYGRAIVVNEWYIT
                     AYEPLYDGNKQWIGALYFGIRHDSVQDLRRQLMEIKVGKTGYVYVLDSKGHYVISKNG
                     ERDGENILEVKDARGNFFIKEMIAKAQAAKVGSVFEHEYPWRNPGEQADKQKVAKLIY
                     FQPWDWVIGAGTYVDEFFEARDMIVTSGNRNLLTGMILALAAALAAALIWFFISRNLS
                     NRLSLFTNQIQHCAQEVSAVSHQVSTSSQALAEGSSQQASSIEETSASLEEMSTMTRQ
                     NADNAGQANRLMDETNEVVSVAATGMQDLTVSMNEISRASDETQKIVKTIDDIAFQTN
                     LLALNAAVEAARAGEAGAGFAVVAEEVRNLAMRAANAAKETSALIEGTGRQVRAGSEV
                     VGRTAEAFKKVAASASKVRELVADIASASSEQAQGVDQINAAVSSMDKVVQESAARAE
                     ESASASIQMSAQAQELTQMADELAHLIAGSKVFQREVRNQAPDARSETNGKPPKEFRR
                     TQALQQAERLRISQVLPLDPEEEAAPPQPRRKVTNSGPKQARIGSF"
     gene            44229..45890
                     /locus_tag="PLM79_02780"
     CDS             44229..45890
                     /locus_tag="PLM79_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HOV85257.1"
                     /translation="MTAVLLQTLASDYFRRLSRRFPVMCAGDEFHAFPRALAANEFLD
                     RMEQLSPEEIEESIAVLKEFQRQFDAPLYPEEGFEESLDRQLLSAGIAGILLELESSE
                     SWRHDPSLYLKIAFVGLDHALTKPCDDSSQVAWRTLARLDKIPSLLRRGAENLRLVPR
                     THHRAALLMVRDCREYLSELAGIAWFESPSRYSGALQEVRTSLEDFEKALNDLAPCED
                     DRICAPKLETVLAHRFLWDRSIEEVREIGELEWEDSLNELERLRREINPGQSWQEIYH
                     SYLPPEVRSVEVFTLYEREMGRLFSFFTRHGLGESLSESLPLIRETPRYLRSLRGSGS
                     FAAALGADPREKDFFYLTTRPLARQSAESRESLVARLHREYAFLAAHETVPGHFLLDS
                     VRRKIPNPIRRQMESPLFYEGWATYAERLLGDYGYVQGPMDCLVHARRRLWRAARCRI
                     DVGLATGSLGKGEAVALLVRTGISAEDAARQVRRFQLSPGYQLCYTLGCHEILRLRSA
                     HGNRLGLNRFHREILEGGQLPFGIIDQRLEKIGTFQEEPGRRNSR"
     gene            45887..47278
                     /locus_tag="PLM79_02785"
     CDS             45887..47278
                     /locus_tag="PLM79_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85258.1"
                     /translation="MRSFLGYHSEWNLGSPGGWDYQRVTQIIGKAVWQRIQQIRPVSV
                     DLDFDHPFLFPVYGFVEMLVEAHRSRSGNAPGLIAVVAEEETLEDVTENINLARHLDR
                     VDGIEGALLAPQALELKNGVVCCRGKAVSLAFVDFNSDVLLSLHRKHDLTPLLQLVRE
                     GRVINPRGTEPINGKSMFEVLTGDDSGRFHPEITRRTPWTRRFYPRSTRGPRGQRIDD
                     LIDWTREHWIDLVLKPERGYSGKGVRVGETHPDAEEAIRLALADGSYIVQEKISLPLW
                     AEEIPTLRREDETIRLERYQTDFRCLIGHQGLLGFLGRYGGVPTNVGSGGGVQPLAVL
                     RSPMSVRDAVERANEAVLSMDYGDLEEVLRLQERMAVEHRFTYLLGPIKIALRPRIIR
                     PEQIEALAGYCEAMWLDCLELEKMWLSGELDEIIRIEPEELEIARMQPWRGQPAIIAS
                     DGLFSFGAHPEMP"
     gene            47275..48093
                     /locus_tag="PLM79_02790"
     CDS             47275..48093
                     /locus_tag="PLM79_02790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV85259.1"
                     /translation="MTVTVDPDWWKTLFDEIYLLTDARSVCDGEITRREIDLICGILP
                     MRPEHRVLDLCGGHGRHSLELCSRGYRGCVLLDYSPSLLSVAQAEALARDLPVRILRA
                     DARNTALVSESFDHVLILGNSLGYAGDDRADRQVLGEAFRVLRPGGWVLLDLADGDSL
                     RDSFNPEGWFEIGGDWVVCRRRHMEGDRVSVREVVLSKEKGLVRDRSYSIRFHDARSA
                     AALLVDMGFADVQVVRDFVLREERGDYGFMRQRMLAVGQKTGKPYRAGGGLVGG"
     gene            48166..48915
                     /gene="gpmA"
                     /locus_tag="PLM79_02795"
     CDS             48166..48915
                     /gene="gpmA"
                     /locus_tag="PLM79_02795"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOV85260.1"
                     /translation="MKKLVLLRHGESLWNRENRFTGWTDIGLSPKGIEEAVEAGRVMK
                     KEGLVFDVAFTSVLARAIKTLWLALEEMDLMWIPIRNHWRLNERHYGALQGLNKVEMV
                     ELHGMEQVQIWRRSYDVPPPALTPEDPRNPANDPRYASLAPGEVPLSECLKDTVARFL
                     PYWHDTIAPVVAQGKRVLIVAHGNSLRALVKYLDNVSDREIVGLNIPTGIPLVYELDD
                     DCKAVRHYYLGDEEAVKKATQAVADQLRKNA"
     gene            49162..50814
                     /gene="pgi"
                     /locus_tag="PLM79_02800"
     CDS             49162..50814
                     /gene="pgi"
                     /locus_tag="PLM79_02800"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOV85261.1"
                     /translation="MVLDRVDPTRSRAWQRLKDHYQVMRTRHMNDLFAEDPDRFRRFS
                     FRFGDMIVDTSKNLITEETMNLLTDLARECRVKESAEKMFSGEKINETEDRAVLHVAL
                     RNRSGLPICVDGKDVMPEVEEVLEQMEGFSRRVLTGGWTGFTGKPIRDIVNIGIGGSD
                     LGPLMVTEALRPYWQPGITPHFVSNIDGTHIVENLKNLSPETTLFLIASKTFTTQETM
                     TNAHTARKWFLERGGEESAVARHFVAISTNEKEVVRFGIDPSNMFRFWDWVGGRYSLW
                     SAIGLSIACTIGFDHFSQLLQGAHVMDLHFREAPLEKNIPVVLALLGIWYGNFFGAET
                     EAILPYDQYLHRFPAYFQQGNMESNGKSVDRRGRTVTYQTGPVLWGEPGTNGQHAFYQ
                     LIHQGTRLVPCDFIAPAVSHNPVGDHHRILLSNFFAQTQALMRGKSREEVQEEMRKAG
                     MSEKEVQRLAPFKTFPGNRPTNSILIRKLTPFTLGSLIAMYEHKIFTQGIVWNLFSFD
                     QWGVELGKQLAGRILADLMEDSPTEAHDSSTNGLINLYKEWR"
BASE COUNT        10257 a        15135 c        15571 g         9915 t
ORIGIN      
        1 cacgcagcag gatgagtcgc gtgtcggcca ggttcaccaa aagggaagca aacaggacgg
       61 acttggtatc gcagtccccg tgcccgttgt agagggtctc caagggcatg gtcagaccga
      121 aggtccggat caggacaccg tctcgtcccc gccgggtatc cggcggcagc cgatattcca
      181 gggactggac gaaagaggcc gaaagttcga caaaccgccg gagatcgtcg tagcctgcct
      241 gcagtcccac gtcccgcaac cgggcggccg cctcccgcac atcttcccgg ctcctttcaa
      301 cgatccaata gtagtcgggc tcgaggctgt tgggcgacgg atcccgggcg atcccgcggg
      361 ccgcgcaccg cagggtctgt tccttctcct tccgccgtct ttcctcctcg gtccgaaagc
      421 tgtaccgcat ccacgattcc ggaatcccat aggattcgac ggccttttgc aggcgcaggg
      481 gatcgatcac gacctcgagg gtgaaacgga gggcgggaaa gcgggcgtca aagatctgcc
      541 ggtctccttc caccctcagg agcctccagg tgttcacgtc ttcccaggca aaccgccgag
      601 tcacggccgt cttgggcaaa gaggggtaca gttggtcggg ggggacccga tccgtcatga
      661 gggcatcgac gaccggcagg catgccaacg atacgagggc cgcggctacg gcgctcgaaa
      721 accatcggaa gcggcacttc gggatcatgg cattcccatc acctgaatcc gttcgaagga
      781 cggcatgggg accatcgccg acagcagact ttgcacgccg tgggaaagaa acagccccat
      841 gaccacgatg agcacgccca gaacgagtgc aacggccagg ttgttcctcc cgatttcaaa
      901 cagttcatcc acttcccgag tgagcctcag gaagatccgc aaagcgctcc agatgccggc
      961 caaagcaatc ccggaggaaa agacgaagga aaaaaggctg atccccacgc aaaacaacag
     1021 gtccccgccg ctgaagcccc gatggaccag ggtctgccac gtggagaccg atggataaag
     1081 agctgcgcgc accacgaagg ccgtggaaag cacaatggaa gcgagcagaa tgcccatgga
     1141 ggcattgttg cgcttgagct cctccatctc gtccaggtgc cgggtgaacc gtgcaaagga
     1201 gcgaaaggcc aaccagacgg tgagaaaccc caaaccaaag gcgagcagca gctcgagtat
     1261 ccccaccagg attgcattcc aatccatcgt tcctccctcc gaatctcacc tcgtttgcga
     1321 tcctgacccc aagcgagcgc agatgaccgc aaaggaccgc agaagaccgt ccataagcca
     1381 aaaggaaagc ggtcccatcc tccctcaagg aaagcctttc gtccacccct aagtttgcaa
     1441 gaaatgtaat gccgcggaac agaggagcag gctcgaaaga aagcctggac tttgtgctgg
     1501 ttcgagcgat ccggcgggca ggaccctgct ttcagggttc ctgggagagg gaggctttca
     1561 ttcaaaggga cccaggctcc ttgaatgatt gatttcattt ggatagactt caaattttgc
     1621 cgggggactc ccagagtctc acagttccgg gagtctgttt tgccggtagg cgctgcagca
     1681 ccaaccaagc ctgaaaaccg tattccggtc acactgttac gtccgccttg aatcatgatg
     1741 atgaaatgaa atacaactga gtcggtgggc gcgtagcagc acgagtttac atagtgcttc
     1801 aagccgcttt gagcggccca cggtctttac gggcctccgg ctttgccgga ggcacctgac
     1861 tccagtgcag gggggaagcc gggaaaggcg accgtagttg ctggaggtcg gcccttgcgg
     1921 gcggggacgg gggggaataa gccatacgga gacctgaccc ctcaacgatc tgcgaatgca
     1981 agacccgtat atggaaattg tcgccgcccc tgcccggcgg cgggggctcc caggtttctc
     2041 tggaatgcct cgagattccg ggggggcggc cccaggccgg gtttacccgg tcccgggaga
     2101 gcctcgatga aatccttcgg tctccagtat cgagaacgaa ccggcaccat cggtccattc
     2161 ccgacagtgc gcgccccccg gcgaggtgtt tccgttcggg agcttgttta ggggaaagat
     2221 catgaccctc atagcctcga tccacggcag ccgcaccggc atatccgcct cttggctcct
     2281 ggttgccttt atccttgcag gtcttgtggg aaaccacttc cctttctcca ttctcaacgc
     2341 acacttcgtc ttcggcagca tcttttccat gctggccttg caggtcctgg gctggggccg
     2401 aggggtcctc gccggtgccc tgatcgcggc ttacacgtac gtaacctgga accatccctg
     2461 ggcctttgtc accatgacgg ccgaggcagc ggccgtgggt tggctctttt cccgccgcag
     2521 gatcaccctg gtggccgccg acgccattta ctggctcttc ctgggcatcc ccttgggcta
     2581 tctttgcttc cgcctgttct cggatcttcc cgcaagcaac gctctcttcc tcgtaaccaa
     2641 gcaggggatc aacggcatcg ccaacaccct ggcggccagg ctgatcctta ccgggctctc
     2701 cctgcgattg aacaccgtcc gcctctcgtt tcgggaagcg gtgtcaaacc tgctgatttt
     2761 cttcgtgctg tgtccgtccc tgatcctgcc ggccttgggc ggcaaagccg acctggacga
     2821 gacggaccgg cagatccgca gctccctcgt ccgcgacagc cgacaggccg ccgggaacct
     2881 cgatatgtgg gtggaggatc gaaggcgggc cgtcgtgcac ctggcggagc tcgccggaac
     2941 ccgttcttgg aaagagctcc aaccgcgcct cgagcaaacc cttgcctcgg acgccaattt
     3001 tcttcacatt gccctcatcg atcgggaagc gacggtcgtc gcccactctc ccatttcgga
     3061 tgaactgggt cggagcaaca tcggaaagag ctgcgcggat tgtccgtacc tggcacggct
     3121 caagcaagac ctgaaaccga tgctcgccga agtggcgatc tcaaggtttg gccgtcccga
     3181 ccccatcgcc atcatggcgg ctcccgtggt ccttggcggt tcctatgaag gagccgtggg
     3241 cggcattttg aatcttgacc ggatccggat catcctcgag accagttccc aaggctccga
     3301 caccttgtac accctgctgg acaggaacgg cgccgtcatc atgaccaacc gccccgatca
     3361 aaaggtcacg acgccctttt cccgcggcag aggcaccctc catcgcctgg aagaaggaat
     3421 gtttcggtgg gttcccgaac tgccccccgg cacttccacc atcgaactgt ggggacaatc
     3481 cgcttatgcc atggaatcca ccgttgggga tttggcggaa tggaggttga tcctggagca
     3541 gcccgtagct ccctttcaga ggaggcttta ccggagctac accggcggct tcttcctggt
     3601 gttcgtcatt ctcgttgcct ccctggtccc ggcccaattc ttgagccgca tggtggtcga
     3661 gactacggag aaactcagct tcctcacccg ggaccttccc gcaaaacttg cctcgggcga
     3721 tacggtcacc tggccccgaa gcgccatcct ggaaaccaat cgcctcgttg ccaatttcaa
     3781 agagatggcg gaatctttgg ccgcccggtt cgccgagagc cgccggatga acgagtggtt
     3841 ggaggggcgg gtggaggagc gcaccgaaga gctgcgcaac agcgaggaat tcttgagcaa
     3901 tatcgtggaa aacatcccgg ccatggtgtt cataaaggat gccgcggatc ttcgcttctt
     3961 aatgttcaac cgggcaggcg aggagctgtt gggctattcg agggaagagc tgttgggcaa
     4021 gtcggactat gatttcttcc cccgggagga ggccgatttt ttcacggcca aggaccggga
     4081 agttctttcc accggacgac tcctcgaaat tcccaaggaa accattcaaa cccggcatcg
     4141 aggggagcgc atcctgcgta ccaagaagat cccggtcttg gatgcccggg ggaaagcccg
     4201 gtacctgttg gggatatccg atgacatcac ggatcaggtc cgactgcagg accaactgtt
     4261 ccaggcccaa aagatggagt ccgtgggacg gctggcaggc ggtgtggccc acgatttcaa
     4321 caatatgctg ggacttatcc tcggccatgc ggaaatggcc ctggatgagg tggatccggc
     4381 ccaaacgatt caccacaatc tccaggaaat tctcacggcg gcccatcgtt cggcggacct
     4441 gacccgtcag ctcctggcct ttgccagaaa acaggccgtc agtccccgga aactggatct
     4501 gaatgagacc gttggaggaa tgctcaaaat gcttcgacgg ctcatgggcg aggacatcga
     4561 cctggtctgg aaacccggct tggacctgtg gcccgtcagg gtggacccct cccagatcga
     4621 ccaggtcctg gccaaccttt gcgtcaatgc ccgggatgcc gttgtcgggg ccggaaaagt
     4681 gaccatagaa acgggaaacg ccacgtttga cgaagcttac tgtcgggagc atgcaggttt
     4741 tttccccggt cggtatgtcc ggttggcggt gagcgacaac ggctgcggaa tggagaaaga
     4801 ggtcttggaa catctattcg agccgttctt caccaccaag gaactgggga aagggaccgg
     4861 cttgggcttg gcgaccgtat acggcatcgt caggcagaac agcgggttca tcaacgttta
     4921 cagcgaaccg gggcagggaa cgacgttcaa gatttacctg ccgcgctgcg acatcgaggc
     4981 ccccgaggaa cctccctcac caggggaaaa gagggacctg cgaggaaccg aaacggtcct
     5041 cctggtggaa gacgaagaag ccattttgtt gttggccaag accatcctcg agcgctacgg
     5101 gtaccaagtg ttcacggccc ctaaccccgc cgccgccatc catgtggcgg aaaccctttc
     5161 ggatccgatc cacctgctga tcaccgacgt gatcatgccc ggaatgaacg gaaaagacct
     5221 cttcgaacga ctcaacctct tgaggccggg cctaaagtgc atcttcatgt ccggctatac
     5281 cgcaaacgtc atcgctcacc acggggtcct ggacgaggga atccattttc tccagaagcc
     5341 gttttcggtg gaagccctgg ccgagaaagt gcgcgaagtg ctggatacat ccggctcgaa
     5401 agtttccccg tcgtgatggg aggcgccgac cgacggtttc atgtcggcga cggcggacga
     5461 tccgggaaag aatccctcgg aaaaaccccg atgttgccct accggcgcgg gactcccggt
     5521 acttccgtcg gaagactctt tcccacgggc gggaaatctc ctccgtttgg gatgagtaca
     5581 tccaacgcgc ctgcacgatc gggtgccgaa tcgtcttgtt tccgggattc cgtacaccgg
     5641 gtaccggtcg acctgcccgg gagagcggcg tccggaagcg ggggaggagg ccgtatgaga
     5701 tgaatgggat ttcccggtca gcgggtcgcg gcaggtttcc tcgaagagga acctccttgg
     5761 ggtgaaaggc gttcccggtc ctggggtttc gttcggcacc ttcgaattcc gcagtcgggc
     5821 gatggcctcc cgcttgggtt ttcgaggcgc ggcaagagaa ttgcttaggg aggaggatct
     5881 tttcggagtt tcggaccgtt tggacaagat gccccggcgg ctccggtgaa gaccgtatcg
     5941 cggcggcaac agcggcaacg gcgccaacaa gagcaggagg gagggcttga agatacaacc
     6001 caaatggaga cactggaaga tccgcacccg ggcaggcgtt gcgactctgg tcggctttct
     6061 cgttgtgggt tccttgtcga tcttcctggt ccacaagcaa atccttcggc aggccgttcg
     6121 agaggccgaa atcaaatccc agttgatgct ggatcgaaac ctggccatcc acagctattt
     6181 cacccaccaa ctcaaacccc gcttgcttcc cattaccgac gcctcccagc ccagggaata
     6241 cttcgacccg gtatggatgt cgtccaccta tgccgtgcgc gagattcaga agtacttctc
     6301 tcaactggga aacgacacgt attactaccg ggaatgcgcc gtcaacgccc gcagtcccga
     6361 aaacgaggcc gacgagatcg agagggattt cattcggagg accggcctcg accccgccct
     6421 ccggtatgag agcctcatgc gaagaatcga tgggaagccc tacctgcagg tgctgcgccg
     6481 gggggaagtg atggaggagg cctgccttcg ttgccacacg actcccgacc gggcccccga
     6541 aggtctggtg aaggagtacg gggccgaacg aagctttcat cgcagcctgg gtgaggtggt
     6601 ttcggccatc tccattcgca tcccgttggc ggcagccttc gcccaggccc gggatgcggc
     6661 cgtccggcta tcccttctct tggtcgggct cctttcggtt ctttccgtca tccagttctg
     6721 gagtgtcgaa cggttgatcg ccacccccct ggcccgactg cgggatcaag cccggagaat
     6781 ttcgtccgga aaggacgaac ccggcggccg aatccctcgg cctgccggcc gtgagctcat
     6841 cgagttgacc gacgcgttca atcacatgtc ttcgaggctt cggcagcacc acgacgagtt
     6901 ggaaggaata atccgggaac gaacggcccg gctgctggcc ggcgaggagt tccaacgggc
     6961 cgtcgtcgag tgctcccccg tggcgctcat cagcaccgat cccggcggga acgtgctgac
     7021 ctggaacgcc tcggccgagc agatcttcgg ctggtcggcc caagaagttg cgggccggcc
     7081 cctgcccatc gtgcccgaag gcaaagagga agcattcggg gaactctgca aacgcctcct
     7141 ggcgggtcgg accttttccc gcatggaagt gaaggcaagg aagaagccgg gcgagctcct
     7201 ggatgccgcc ttttccgctt cgcccatccg cgatgccggg ggcagcgtca tcggcatgat
     7261 gtacgccgtc gaggacatca ccgaccgcaa gcgggccgaa aaggacaaga cggccctgga
     7321 aacccagttg cgccagaccg ccaaaatgga agccgtgggc aggttggcgg ggggaattgc
     7381 ccacaatttc aacaacattc tgggcatcgt tttgggcttt gccgacctgg ccgttctgag
     7441 cctggagaac gacaaaaccg aggcccgagc ccacattgcc aagatcaagg acgccgccgc
     7501 cagggcttcc ctcctggtcc gtcaactggt gacgtttagc aaaaaacagg caccgcagcc
     7561 ccaggtggtg gatgtcaacc tggcggtgtc cgaaatggag aagctcctcc ggggaatcat
     7621 cggcgaagat atcgagctga tatccattct cgacccccaa ctctttcggg tttccaccaa
     7681 tccgggcagc atcgatcaaa tcctcatgga ccttgccctc aacgctcgag acgccatgcc
     7741 cgtcggcgga aggctcacca tcgagacccg gaatgtcttc ctcaacggaa cggattccca
     7801 cgggcccttg ctcgctcggc ccggccctta cgtgatgatt cgagtcacgg ataccggcac
     7861 gggagtcaaa gagagcatcc gggaccgggt gttcgagccg ttttttacca ccaaggaggt
     7921 gggggacggg gtgggattgg gcctttcttg cgtgtacggg atcgtcgagc aaagcggcgg
     7981 atatgtccgg atggacagtg aggagggaag aggcacgacc ttcgaggtgt tcctgccggc
     8041 ggttgcccaa gaacccgagg ggaagacccg ggagtcgctt cctgtcctac cgtcggttca
     8101 gggttcggaa accatcctgg tcgtggaaga cgatgagagg cttcgcggga ccgtctgcca
     8161 ggtactcgag aaagcgggct accgtgtctt gcaggccgaa aacggaacgg cggccatcga
     8221 gatcgtgacg gaataccccg gtcccattca cctgctgctc acggacgtgg tgatgccggg
     8281 aatcggcggc cccgagctgg catcccgcct ccggggtctc aagtccgaaa cgaaggtcct
     8341 atacatgtcc ggctacacgg accatccctc gggcaatcgg gaggcctccg gctctccggc
     8401 cgcctttctc ccaaaaccgt tcaactcctc ccaactgacc caacgagttc gggaggaact
     8461 ggaaaaggac tcttcctaac tcggaatccc ttggtgcaac cggaaggtct tgtatgaaaa
     8521 cgccccgtaa acgagcgcaa cgatttccga aagacgatgc gcaacctgag ggccggggga
     8581 gcgctccccg gtccccgggt cccgagaagg attccctcga ttgcctgatc tctttttcga
     8641 gggagctctc ctcggctcgc agcctcgctg aactcatggt tgccgtgcgg aacgcggtcc
     8701 gacagctcac cggcgcggac ggcgcgacct tcgttctgcg ggaaggagat ctttgccact
     8761 acgccgacga agatgccgtc ggtcccctct ggatggggag ccgctttccc atggatgcat
     8821 gcatcagcgg ctgggtcatg aaggagaaaa aacctgcggt catcgaggac atttacgccg
     8881 atgagcgcat cccccaggac ctgtatccac ccaccttcgt caaaagcctg gtcatgatgc
     8941 ccattcgctc tgcggatccc atcggagcca tcggcgtgta ttgggccgag cgccaccgtc
     9001 cgggagacgg ggaggtgaag attatccagg ccctggcgga gagtacggcc gttgccctgg
     9061 ggaaccttca actcctcgag gaacttgccc gaagcgccgg ggaggtccga gacttcctcg
     9121 aaaccctggt gaagacttcg gagagcctca tcctcgtctg tgacccgggc gggagaatca
     9181 ccctgttcaa ccgagcctgc gaggaactca gcggatacgc ccgagaggag gtcctgggga
     9241 aaaccgtttt ggagcttttc cagggggcgt cgaggccttc ccctggcggg cgggggtttg
     9301 tgcatccgtt cgatccctgc ctgcgactcc ctcagctcca ttcgtggaag acaagatcgg
     9361 gggaagagag gctgatcgag tggaggtgct cgttcctccc ccacccggag gacgacgagc
     9421 cccggtccca ctcctttgaa ccactgcctt ccgggagcga gcgattcttc cagctgctct
     9481 tcgaggaggc cccgcttccc taccagtccc tggacgaaac cggctgtttc ctccatgtga
     9541 accgggcctg gctcgaacgt ctgggctacg ccctggagga agtcctgggc aggaacttcg
     9601 ccgaattcct cgcgccgcag agtctggacc gattcaagga aaatttcccg cgcttcaagg
     9661 ccctcgggga ggtgcacggc gtcgagtttg acatggtaag aaaagacggg tccgtcatgc
     9721 aggtatcctt caacggcaag gtcgcccggg acatcgaggg gaacttcatc cggacccact
     9781 gcatcttcga ggacattacc gaacgcaagc gcgcgcagga ggagaagaga cggcaacagg
     9841 acgtgctgtc gagggttttt cgagcctcgc cctatatcat gatgctggtg gaccgggagg
     9901 ggcgtgtcct caaaatcaac cgcgccgggc tggagttctc gggaaggtcg gagggagagc
     9961 ttctgggact ctcgggagga caggtcttaa actgcattca tgcccatacc gcgccggttt
    10021 gcaaccggag tcccgcctgt ggggattgcc tgatccgaaa tcggatgctg cacagctttc
    10081 aaacgggaga gagcaccttc gacaccgaat gcagcctgcg aattcgtaag ggggctaccg
    10141 aaggagtcat ggacatgctc ctatccacca ccctggtcaa aaacggcggg acggataccg
    10201 tgttgctcac cattgccgac atcaccgagc gcaagcgcaa cgagagaaga ctgagggaat
    10261 acgaaaaggt ggtggagagc cttgatgaaa tgattgcggt ggtggaccgg gactaccgct
    10321 acctgatcgc aaaccgggcc tttatgaaga atcgggcgcc ggggaccggc gaagttctcg
    10381 ggcgaccgat cccggagatt ttgggtgagg aaatgttcga ggagatcgtc cgggaaaggt
    10441 tggacgagtg ttttcggggg aaggtggtcc gctacgagat gcaatgcccg caccccgggg
    10501 tcggggtccg cgacctgtcg atcacctacc accccgttga aggtccggga ggggtggatc
    10561 gagcggcctg tatcatgcgg gacgtcaccg agcagcggcg aggggaggaa gagaaaaaga
    10621 agctccggga ccagctgtat cagacccaga aactcgaggc catcggcact cttgccggcg
    10681 gcatcgccca tgatttcaac aacatcttgg ggatcatcct ggggtttgcg gaaattgccc
    10741 tgggagagca tgcggagcgg ggcctggcgt cgggggccct ggaccagatc gtcaaggcga
    10801 ctcatcgtgc cagggaactc gtgaagcaga tcctcacctt cagccgccgg acccaacaag
    10861 atcgaaggtt ggtgcaggtc tcttccctgg tgaaagaggc actcaaaatg ctgcgggcct
    10921 ctctccccac caccatcgag atccggtcag acctccaggg ccaaggcatg gtcaatgccg
    10981 acccggtgca aatccaccag atcatcatga acctttgcac caattcctac catgccatgc
    11041 gtccccacgg cggggtgctg acggtgggcc tcgccgacgt ggaattcggg gagaggactc
    11101 cccacccgga cctccccaac ggtgcctaca tgcggctgac cgtgagggat acgggctccg
    11161 ggatcgatcc cgccatcctg gacaggattt tcgatccgtt cttcactacc aaggacctgg
    11221 ccgaagggac gggaatgggg ctctcggtcg ttcacggcat tgtcaagagt tacggggggg
    11281 gggtgaccgt ggagagcgaa ccgggaaagg ggagcacgtt tacggtctac ctgccccggg
    11341 tccaggccca gcccggcaag catttggaaa agctacgccc tgcattgccc gggggaaaag
    11401 aacggatcct tttcgtggac gacgaaaccc tcctggcgga gttgagcgcc aagatgctca
    11461 gcgcgctggg ctaccaggtg gtgacggaag cgagcagccc ggcggccctc gagctcttcc
    11521 aacgggagcc tcaccggttc gatctcgtca tcacggacct gaccatgccc caaatgaccg
    11581 gcgacgaact ggcccggcga atgatggaaa tccgcaggga cgtgcccgtc atcctgtgca
    11641 cgggttacgg ggaaatgatc acccgggaac aggcgcagac aatggggatc agagaactgc
    11701 tcctcaagcc cctttcccgg gatgaccttg ccctgacggt tcgaaatgtg ctagacggga
    11761 ttgcggcggg ttcgcagcgt cgcggcgacc tttccgattg actctccgga ggctttcggc
    11821 cgttccagcg atctcatctc ctcgacccgg gcctgccagg tatcttcacc ctcacatacc
    11881 cggcccgtgt gacgacctcg ggtgcctccg tccaagccgg ttgcaccaac cctataacca
    11941 gaggattttg aacccttttg gaccgggaaa tagaggtccc tgcaaccccg gagacgacca
    12001 tggaaaacgc cctctcacga aatgtcctga ttgtggatga cgaagaggag tatcgggagt
    12061 tgatccgcag ctatctgagc aaacgcgggt tcagctgtga agtcgcttcc aacggagagg
    12121 atgccctggc cctcttgcag aagaagccgt ttgacgtcct gatcaccgac attcgcatga
    12181 aagggatgga cgggattcgg ctgatgaccg aagcccacaa ggggcaaccc gacctggacg
    12241 tgatcatcat tacggggtat gctccggcat actcgtacac ggagatcgtt cacgccgggg
    12301 cggcggactt tatcgccaaa ccgttcgaaa tcgaggaact acatgcaaag ctggaccgaa
    12361 ttcggaggga acgccgggct ttccgacagc tccagttggt cttggagcag agtattcgcg
    12421 cgctttcgct ggccatggaa ttcaaagacc cctatacggc cgggcaccag cgccgggtgg
    12481 cggcccttgc cgaggccatc gccctcgagc tgggactctc gagaggggtg gtggatgccg
    12541 tgaggatggg cgggctggtc cacgacatcg gcaagatttc ggtcccctcg gagatcctgg
    12601 ccaagcccgc aaggctgcaa ccggtggaat ttcagctcat ccggcagcac accgaggcgg
    12661 ggttcgagat tctcaagaac gtcgatttcg agtggcccat cgccgagatc gcctttcagc
    12721 atcacgagcg gatggacggc tccggatacc cccgaggact gacgggggac aagaccctgg
    12781 tggaggcaag aatcatcgcc gtttccgacg tggtcgaggc catgagttcc caccgtccct
    12841 atcgggcggc gttgggcatc gatgcggctc tcgaggagat ctcccgtggt cgagggactt
    12901 ggtttgaccc ggaggtggtg gatgtttgtg tccggctctt cagggaaaag cacttcgaat
    12961 tcaacgctgg gggctgaacg gcgcttttgc cgcgattcgg tcgcttcggg ccgcgttgcc
    13021 cgatcctcgg tggaacccct cagccgagag gggatcaagg gggggaaatg aaagaacctg
    13081 ccgagacacg gcggatcctg gtcgtggatg atgaggaaga gtatcgcgtg ctcgcgacca
    13141 cctttctgga acgatccggt tacacttgcg cgtcagcctc ggatgcggac gaggccttga
    13201 ggaagctcga cgagcggccc ttccacctgg tggtctcgga catccggatg aaaggaaagg
    13261 acggactgag gctcatggcc gaggcccggg gtactcatcc ccacctggat ttcatcatca
    13321 tgaccgggta caccgaggac tacacctaca gcgacatcat caaggccggg gccacggact
    13381 tcatccacaa acccttcgac atgcatcaac tcctggccaa gcttcaacgc atcgagaggg
    13441 aaaaatccac cctcgcccgc ctacaggaag cctacgaaac cctctcgtgg gaggccgagg
    13501 tcaaccggtc ttttgcggag ctctcccggg cactgatctc cgcccggccg ttcgaggaca
    13561 tctcctcgct ggtgctggaa cacgcaaaag ccctgaccgg cagcccgttg ggctatgtga
    13621 gccacgtgga ccgggagacg ggccatctga cggtttccac catggcagcg ggcacgtggg
    13681 agctctgccg ggccgaagac aaggatgcgg ccttcagaga ctttcacgga ttgggggccc
    13741 gggtcctgga gaatggaaaa tccctgcgga tcaatgattt tcaacgggaa ttgccctcgg
    13801 ggagactgcc ggcgggtcac attccaatcc accggttcct ctcggcggtc gcgaccgtcg
    13861 aggaccggat ggtgggccaa gtctccctgg caaactcttc ccgggattat tccgaaaagg
    13921 acctggtagt gatcgagcgc ttggccgacg tgttcgccgt ggccgtacag cgcagacaga
    13981 tggaagacag ccttcgaaag tcccacgggg aaacggaaca gctcttggcc tcgatcccct
    14041 ctcttctcat cgaactctcg gaggaagacc gggtggtgcg ctggaatccc gcagcccgga
    14101 gggtcttggg aaaatccctc gaggaagtgt tcggcctgcc cctcgaggaa gtctggatgc
    14161 ccgtggattg gaagcgggtc cgggcaggca tcgccgcggc tcggtccacg gggaaatccc
    14221 aaaggctgga cgacgttcgg ttcctgcgat ccgacgggaa ggaaggcttt cttagcctct
    14281 ccattagttg cgttcaagac cacagcgccg ggaaaacggg gctcattctg ctggggtccg
    14341 acgtgaccga gcgaaaaatt ttggaaggcc agctggccca ggcccagaag ctcgagtcca
    14401 tcgggcagct ggcggcggga atcgcccatg aaatcaacac acccacccag tatgtgggag
    14461 acaacgtgcg ctttctccag gacgccttca aagacctgga acggctgttg gacaagtacg
    14521 aagaactgcg ggcctcggtg cgcgacggtc cgcccactcc ggccgtgatt tcggcagtgg
    14581 aagaagccgt tgagcagacg gacctggact acctgaaaac cgagatcccg aaggccattg
    14641 aacagtcgtt ggaaggaatc cggagggtct cgaagatcgt tcgggccatg agagagtttt
    14701 cccacccggg tctcaatgaa aagacggccc tggacatcaa ccgcgccatc gagagcaccc
    14761 ttacggtgac ccgcaacgaa tggaagtatg tggccgaggt cgtgaccgac ttcgacccga
    14821 acctgcccct ggttcccgtc ttgccggggg aattcaacca ggtcatcctc aacatgatca
    14881 tcaacgcggc ccacgccatc ggcgcagtgg tcggggacgg ctccggggcc aagggaacga
    14941 tcacccttcg gacccggaaa cgggacgata gcgtcgagat ctccattggg gatacgggaa
    15001 cgggaatccc ggaagaaatc cgctccaaga tcttcgaccc gtttttcacc accaaggaag
    15061 tgggcaaagg cacggggcag ggactggcca tttcccattc ggtcatcgtc gagaagcacg
    15121 gaggcaagat ttccttcgag tcccaggtgg ggaagggcac caccttcttc atttgtctgc
    15181 cgacccagca ggaccttcaa tcggagcgac gtcatgaaaa agagcattct ttttgtggat
    15241 gacgagccga aagtcttgga ggccctccag aggtccctgc gtcccatgcg tcgggagtgg
    15301 gacatgacct ttgccgacgg gggagcgaag gccctcgagg tccttgccgc caaacccgtg
    15361 gatgtcatcg tgacggacat gcgcatgccg ggaatggacg gatgcaggct tctggccgag
    15421 gtccggacgc gtcatccccg ggtggttcgg atcattctct ccggccattc ggaccgggag
    15481 acgatcctga agtccgtggg gcctgcccac cagtacctac cgaagccctg cgacaccgaa
    15541 accctcaaga ccaccatcgc ccgggcgtgc gccctcaggg cactccttac ggatgagtcc
    15601 ctggtgcagg tggtctcccg ggtggaatcc ctccccagtc tcccctccct ctattccgag
    15661 gtggtccggg aactcgaatc cccctacgga tcgatccaga aggtggaaca gatcatctcg
    15721 agggacctgg gtatgacggc caagatcctt cacatggcca attcggcctt cttcgggctt
    15781 cctcagcacg tctcgagccc ggcccgggcg gtgaggctcc tggggctcga aaccgtcaag
    15841 gccctggtgc ttacggtcca gatcttttcc caatttcccg aggcccggct gccgggattt
    15901 tctatcgagg acctatggca ccacagcatg accacggggg ttttcgccaa gaagattgcc
    15961 aaggaggaga agcaggaccc ccatgcgctg gacgattcgt ttatggccgg cctgctccac
    16021 gacatcggga agctcgtggt cctggccaat ttccccgacc ggtacgggga aatcctccat
    16081 cgggtccgtg aaaccggggc ttccatgtgg gaatcggaac aggcggtcct gggctccacc
    16141 catgcccagg tgggggccta tctcatgggc ttgtggggtc tgccggactc gattgtggaa
    16201 gccatcgcct atcaccattg tccccaggac tgcctgggcc gcagcttcag ttccctgacg
    16261 gcggtgcatg cggcaaacgc ttttgccgcc catgccccca ggcgggagga gatggaaggg
    16321 attccgcatc tgcgcatgga ctacctcgaa gcgctccaac ttgcccatcg agtgccccga
    16381 tggcgggaga tctgcgggga attgacccgg gaagaggatg ccccatgacg gaaaagattc
    16441 tcttggtgga tgacgatccc aatatcctgg cggccctccg gcggcagctg cggaaggtct
    16501 ttctcatcga cacggctccg ggaccccggg aaggactgga ggccgtccgc gaaagcgggc
    16561 cttatgccgt ggtggtgtcc gacctgcgca tgccgggcat ggacggcatt cagttcctgg
    16621 caaaggtgag gaccctggct cccgataccg tccggatgat gcttacgggg aatgccgacc
    16681 tgcatgcggc catggaagcg gtcaacgaag gcaacgtctt tcgttttttg accaagccct
    16741 gtccccccga agtcctggtg aaggggcttt gggcgggcat cgaacagtac cggctgatcc
    16801 ttgccgaaag ggagcttctc gagaacacgc tccggggcgc cgtcaaggcc ctcatggaaa
    16861 tcctttccct ggtcaatccc gaggcgttcg gaagggcctc gagggtgact cggtatgcgg
    16921 tcgaggtggg gcggaagatg aaggtggcgg aactttggca gctggaaacc gccgccgcat
    16981 tgtcccagat cgggtgcatc atccttccgg agcaagcctt gcggaaggtt taccggggcg
    17041 aaagcctcac ccagcaggaa gagcggcttt tccaaaagca tccgaccgtt gcggcggaac
    17101 tgctggcgaa aattccccgg atgcagaaga tcgcggaaat catcgcctac caggccaagg
    17161 aatacaatgg gggaggtctt ccccgggacc ctcgtcgagg cgacgagatc cccctgggct
    17221 cgaggatcct taaggccgtc ctggacctgg acgcccggga agccaggggg gaacccccgg
    17281 agagggcact catggagatg aaggcccgcc gcggcatgta cgatccggcg gtcctgggcg
    17341 ccatcgaggc ctggatcggc gccgaaacgc ggttggtgga acaaaccctg gaaagcccgg
    17401 aacagttgag cgaaggaatg atcctggccg aagacgtgtg tctttccgac ggtcgcctgc
    17461 tggtcgcccg aggatacccg gtgaatcgga ccatgatgga acgtctgcgc aactttgcaa
    17521 gaaaaccggg cctcaaggca cccctgaagg tgttggtccg gaacccggga ggtatctgaa
    17581 catgtcccgc accattcttc ttgtggatga cgaaccgctc gtgacggaag ccctgaagcg
    17641 agcccttcgc aaggagccgt acctcgtcct gaatgcccgc tcggcggccg aagccctgcg
    17701 gatcctcgag aagcaccccg tggatgtggt ggtctccgac gagaggatgc cgggaatgac
    17761 ggggtcggag ttcttggccg tcgtctgtag gaagtacccg gaaaccatga ggatcatcct
    17821 caccgggcac gccgacctcg agtcggccct gagagccatc aacgaaggcc atgtgtaccg
    17881 ctttctacgg aaaccttgta acgaagcgga actgatccac actctgcgcc aggccgtcca
    17941 gcagaaagat ctggcatcgg gaagccgaag gttgcttcac gcttttcgaa agcaacagtc
    18001 ggaactgaac gccctcgaaa aggaacatcc cggcattcgc accgtaaagc gggatgagcg
    18061 gggggccatc gtcctggagg aggaggagga ggactacgat ctggacaccc tcatcgaaga
    18121 gatcgaacgc gaggtgaggc aggacgagac ccgtccgttg cccccccggt agcggggacc
    18181 gatccgggag agctttcgtc cccccgggat ttccggcggt ccttcgccga agcccgggga
    18241 acctgtttct tttggccgcc caccttcctt catgggtccg gagggaagtt tttgtgattc
    18301 ctcagctctt cgttgccttc ctcaccggct tgactgcggg agggattggg tgcctggcgg
    18361 ttcagagcgg actgctggcc gggtccctgg cgcgccgctt cgaggggtgt gccgaagtcg
    18421 agggcgtcgt gggcccggcg gaaaccgggg ctgcggccga gccccgtttt gcggtgacca
    18481 tcgccctgtt tcttttggcc aaactggcgg cctataccgg cacgggcttt ctcctggggg
    18541 ccttcggctc cctggtccgg atcacccccc tgtcccgggc cgctttgacg atcctcatcg
    18601 gaaccttcct tgtgggaaac ggcctgcgga tgctgggcgt ccaccccgtt ttccggtggt
    18661 tcgtcctcga gccgccctcg agcctcaggc gctttatccg gcgtgcatcg ggaaggaccg
    18721 tttccccggt cgctccggtc tttctgggga ccctgaccgt cctcctcccg tgcggcgttt
    18781 cccaggcgat gatggcagcg gccgtgggaa ccggggaccc gttcctggga gcggctttgc
    18841 tgttcgcatt caccctgggc acgatcccca ttttcttttc cgtcgcctat ttcgccactc
    18901 gattgagcgc cgccgtccag gcgcacttca accgctttgt cgccctcatc cttcttgggt
    18961 tcggcggcgt ctccgtctat tacggcctga acctggcagg gattcctttt gggctttcct
    19021 actcggatca cggcccggcg gagcaatccg gtttctcgaa tccgccgggg ccctcggaaa
    19081 ccggcacaaa cgggaaaggt gaatacgtga tctcggtttc caacgggggc tatgaaccca
    19141 aggtcctgca cctccccgcc aaccggccgg tcaccctcat ctgggtcacc cgggacggca
    19201 cctcctgcac ccggtcggtt gtcctgcccg ggctgaaata ccatgcgatt cttccgtcct
    19261 ccgggcgttc gcccctgacc atcccgccgc agaagttggg cacccggttg ccctattgct
    19321 gctcaacggg taggttcacc ggtcttttgg tcttcgacgg caaagaggag gggacggacc
    19381 catccccgga gggaacccgg ccataggtcc ggggaaatcg acagcgcgca tttgtgagac
    19441 ctgaaacgag gaataggctg tgaaactgcg attccgcgtt ccggacatgg tgtgtccgga
    19501 ttgcaccatg cacctcgaaa gcctcgagga cacattgccg ggggtgaagc gcattcgggc
    19561 cagctatcgg cggctgtcca tggaggtcga attcgacgag aggcggctcg acgcccggga
    19621 aatcattgcg gcggccaacg ccatcggcta ccacccggaa catcctttgg ttggaaaacc
    19681 ggaggcttcc accccgacgt ccgggctgct gctccccggg gaggcgacaa aaagcactcg
    19741 aaggatcgtc ttgccggtga aaggcatggc ctgctcgacc tgcgcagggg ggatcgagcg
    19801 gcaactcctg cggttacccg gcgtcgtgga ggctcgggtg gattttgccg gcgagagggt
    19861 tgccgtaacc tttgatccgg ccgggatcga cctcgacggc atccttaccc gaatccggcg
    19921 catgggctac cgggtcccca ccggcagggc ggtactgtct ctcgatggat tgcgggaaca
    19981 ggacgatgtc gggaaactcg agggcctttt ggccgtccag gacggagttc tcgccgttcg
    20041 ggtaaaccac gcgatccacc gggcgggggt ggaattcatc cccggcgtga ccgatgcggg
    20101 ggaactcgcc agggccatgc gaaagggcgg gttcgaccgg gttcgcacca gggatttccg
    20161 ggaattggag gacgatgagg gcggttctca cgcgaccgag gagaagcgcg ggaaacgcat
    20221 gatgatcctc gggattgctt tcacgctgcc gctggtagtc tatagcatgg ctcaggattt
    20281 cggtctcgtg gggtttgacc acgatttggc ggtgatgctc ctggcggcga cggtggtgca
    20341 gtttgtgccc ggcctgcagt tttacgtggg ggcctatcgc agcctggagg cgggcaccgc
    20401 caacatggac gttctcatcg ccctgggttc gtccgtggcc tatttctcga gcctcggagt
    20461 ggtcctgggc ttggttcccg gtcccaatgt gtatttcgaa accggtgcgt ccgtcattgc
    20521 cctggtgagg ctgggccggt atctcgaagc ccgggcacgg agcacggctt cccaggcgtt
    20581 gaaactcctg gcggacctgg gtgccagaac ggcccgaatc cgccgtgggg aagccgagat
    20641 cgagatcgac gtcgaggacg tggacgtcgg ggacctggtc gtggttcgcc cgggggaaaa
    20701 ggtccccgtg gacgggattg tcgagagcgg acgatcttcc gtggacgaat ccatgatcac
    20761 cggggagtcg atgcccgtgg ggaaagcccc gggagacgag gtgatcggcg cgactctcaa
    20821 ccaggaaggc accttcacct tccaggcgac ccaggtggga aagaacacga ccctggcccg
    20881 aatcgtgcgc ctggtccagg atgcccaggc cgccagggcg cccattcaaa agctcaccga
    20941 ccagatcggc gcctactttg tgcccatagt ggtcgccatg gccatgggta ctttcctggg
    21001 ttggatctat gtggccaaca tcgagtggtc gggggccatg atcaacgccg tggcggttct
    21061 cgtcatcgcc tgcccctgtg ccgtcggcct ggcgaccccc acggcaatcc tcgtggggac
    21121 atggaaaggc gccgagcacg ggatcctctt caagaccggc gaagccctgg aacgggccgg
    21181 caaggtgaac gtggtggtgt tggacaagac cggcaccctc acccggggca tgccggaagt
    21241 gacggacatc gtcccggcgg ctggggagga tcccgatgtc cttctgcgcc tggcggccgg
    21301 ggcggagcga gggtccgagc atcccctggg acgggccgtc gtccgggccg caacggaccg
    21361 ggggctcgat ccggcgcaac cggaggattt cttggcggtg gccggtcaag gggttcgcgc
    21421 cgtggtggac gggcggacgg tgctcgtagg gagtccccga atgatgccaa gggaaggcat
    21481 cgatctcgag gaattggccg gggaggtcac ccgcctcgag tctgccggca aaaccgtcat
    21541 ggccgtggcc gtcctcgagg attcttctcc ccggcctatg ggcctgctgg cggtggccga
    21601 cgccgtgaaa ccggaatctc gaaccgccgt tgccgaactg cagcaaatgg gaatcgacgt
    21661 ggtcatgatc accggcgaca accggcgtgc cgcccaggct gtcgcctccc aggtcggcat
    21721 cgagcgggtt cttgccgaag tgcttcccgg cggcaaggcc gaagaaatcc ggcaactgca
    21781 ggcggcggct cctgctccgg gtctgccccg accggtggtg gccatggtgg gcgacggcat
    21841 caatgacgcc ccggctctgg cccaggccga cgtcggcatt gccgtcggca ccgggaccga
    21901 cgtggccgtg gccgccgcgg gcatcaccct gatcagcggc gaccttaccg gggtgggacg
    21961 cgcaatctcc ctgtcccggg gaactctgca gaccctctac gaaaacctca tctgggcctt
    22021 tttctacaac atcgccctgg tccccattgc ggcctacggg ctgctcagcc ccatgattgc
    22081 cgcaggcgcc atggccttca gttcgatcct cgtcgtgacc aacagcctgc gcctgcgggg
    22141 atacaaagtc cggacctttg aggcgcccaa ggacctgtgg cgccaatgcc tcgatctgac
    22201 ccccaggatc ctggccccca ccgcgaccct ggggatcctc atcgtcctcc ccatgctgac
    22261 catggcccaa ggggcggaaa tccgcggagt ccttcccagc accatgtcgc ccgtcctgat
    22321 gatggtgatg gccattgcca acggcctcat cgccgtctcc tactggtcca tccccgtatt
    22381 cctgctggtc ttcatccacc ggaggaaaga cctgcccttc tcctgggtga tcgtcctgtt
    22441 cggcaccttc atcctggcct gcggcaccac ccatttcgtg catatcctgg gcctgtggtg
    22501 ggaggtggat tggtggcagg ccctggtgga cagcctctgc gccgtcgtct ccctggcgac
    22561 ggccatcgtc atctggccct tgttgccgaa aatcctggcc atcccgagcc cggaacagct
    22621 gaggatcgtg aaccgcgaac tgaggctgga gagagatgcc ctcgaaagga ctcaagcaga
    22681 gttgcgaagg gcctacgccg atatggaacg gcgggtcgag gagcgaacgg cggctctggc
    22741 ccgctccaac gaagccctac ggctcgaaat cgccgagcgc aaacgcgccg cggaagaccg
    22801 cgagcgcctc atggcggcca tccagcaggc gggcgagatg gtggtcatca cggacctcga
    22861 tgggaccatt cagtatgtga atccggcctt cgagaccgta accggctata gccgccggga
    22921 ggccgtgggt cggaatccgc gcatgttaaa gagcggagag cacgacgcag ccttctatcg
    22981 cgagttgtgg gacaccgtct cccgcggcta cgtctggcga ggacgcgtgg taaacaggcg
    23041 caaggacggg tccctcttca ccgaggacgc gaccatatcg cccgtttgcg atgcttcggg
    23101 acggatcgtc aactacgtgg ccgtcaagcg ggacatcacg gcggagatcc ggctgcaggc
    23161 ccaattgctc caggcccaga agatggagtc cgtgggacgg ctggcaggcg gtgtggcgca
    23221 cgatttcaac aacatgctgg ggatcattat cggtcatgcg gagatggccc tggatggggt
    23281 cagcccttcc caacccatct atcgcgagct ccgggagatc ctgagcgcgg cccatcgttc
    23341 ggcggacctg acccgccagc tgttggcctt cgcccggaag caggccgcca acccccgggt
    23401 gctcgatctc aacgagaccg tggagggcat gttgaagatg cttcgacggc tcatcggaga
    23461 agacattgac ctgctctgga agcccggctc gcgcctgtgg cccgtcaggg tggacccctc
    23521 ccagatcgac caggtcctgg cgaacctgtg cgtcaattca cgggacgcta tcaccggcgt
    23581 gggaaaggtc accatcgaga cggggaatgt ggtgttcggc gaggagtatt gccgggagca
    23641 tgggggctat gtccccggag agtatgtaat gcttgccgtg agcgacaacg gctgcggcat
    23701 ggacaagaag gtcatggggc agcttttcga accctttttc accaccaagg aggtcggcag
    23761 ggggacgggc ctgggtcttg ccaccgtcta cggcatcgtc aaacaaaaca gcgggttcat
    23821 taacgtatac agcgaacccg ggcaagggac cacgttcaaa atctatctgc ccagggttga
    23881 atccctcccg tccctgaagc agggtccatc gagcgcgagc cgcgacttga gaggttccga
    23941 gacggttctc gtggtggagg acgaagaatc catgctggag ctggccaagg ccatcctcga
    24001 aaaacacggg taccgagtgt ttgcggccgg cgatccggcc aaggctttgg aactggccca
    24061 aaagcacccc ggacgaatcg acctgctgat caccgacgtg gtcatgccgg gaatgaacgg
    24121 gaaggagctc aatgaaaggg tcgccgcgtt gagccccggt ttgaaatgta ttttcatgtc
    24181 cggctatacc gccaacgtca tcgcccacca cggtgttctg gacgagggaa tccattttct
    24241 gcagaagccg ttctccatgc aggccatggc cgcaaaggtt cgtgaagtgc tggattcttg
    24301 aacgggattg ctccggctga tcttttccgc tggcggctct agatttttgt tgaccttcct
    24361 tcctccggat acagaatagg acccgaacgg aagaagactg gagacgggga atgaaggact
    24421 cggagaaatt caaaacggtt cgattatggg tgttgctcat cctcggttgc gctgccatgt
    24481 tgtgggggta cgccatgatg tagcggctcg gcggagaaac cgggacggtt cgaagaaggc
    24541 cgggtcaaac cggggacgga attccgcgga agcctttcca tatcgatcga tcccaatgct
    24601 cccgaggaag aggacgatcc catgtcgccg tcggcgtttg attacgatca acgagaggcc
    24661 tacagccgga gttgcagcct gatgttccat ccggcacggg gagccgtggt caaggaaacg
    24721 ttcggaacca ggtccattcc tttctgtatc gacctcgagg aaagcacggc gatttcgttc
    24781 ggaggacggc tggcgaccat ctcgagattc gggtttgccg ggtgccaggg agtgacgggc
    24841 aagatcttca agagaggagg caccagccgg gaaaccgttc agaaggagat cgggtcccag
    24901 ctgcgagcca tggacctcga ctgcgtgttt ctcctgggag acaactttta cgacgatgga
    24961 atcggggacg ttcgcggagc gaggttcacc acctgctttg acgacatcta ccctacggga
    25021 gaaagaccgg ttttcatggt cctgggaaac cacgactaca accttcacag tcacggggac
    25081 aacccgcttc tgagctcggg ggccaagagc ccctccgaac ggatgacctg ggccctcacc
    25141 caggtgcagc ggacctatta cctgctccag gcgcgccaca ccagcaaatg gaccatgccc
    25201 taccgctatt atgccctctt ctccagaaac cttcatttca tcatcctgga ttcgaatacc
    25261 tatccctttg atgcctttca acagcaatgg ctggaggacg tctgcgggta ttccctcaag
    25321 agaggcaagg ggaatgtcct tagagccctg atcatgcacc acccggtcat cgcccgggga
    25381 aagcgagcgg cgtccctgga cgacaagaat cgctatgcgg ccactttcgg ggccccgctc
    25441 gaggacagcc gcgcccggcg ggggcttcgc accaacagca tgtcggatta catgtataac
    25501 tccctggtat ccctcaatac ccgtaacctg ctggtctttg acttcattct ttgcgcccac
    25561 gaccacctga cctcgagtga gatcgtcagg atcggaaatc gagaggtcct tcaggtcatc
    25621 tccggcggtg gaggcggcaa cctcgaggac gaaaagaagg tggaaaagtc cgatgccatc
    25681 tacgacggac aggtggacaa gcccgagctc gagcacctca tcggccgcct ggaacacaat
    25741 gaatactgca tcattcaaca cggatatcac gtggccacgg tgtacccgga caagagaatt
    25801 cgattccagt gcatgggaaa atccgatacc cgcacggccg tctacagctt caaacccggg
    25861 gagagggtgg gcgatcatct caggcaaacg gacggcagag cgctgcctcc ggaaatcatg
    25921 actccccgaa aggaaaggct cgaccgctac gttgcgacgc tcaagaacgc catcgagcag
    25981 catacgtggg agttttccgt gttcggagga ggggaaacgg tggaggtcgg tcccggcagg
    26041 agcatcaagg tccccaagaa cgtcaaatcc atggtggacg ccatcaagca atacgaaaga
    26101 aacccgcaca acgtggaaat cgacgtggaa atccagagga ttgcatccct ggccctaagc
    26161 cggggaagcg cattccgggc ggccgacacc acccggtttt atcgggaatg gtccacaaag
    26221 acggtggatg actgcattcg agaggcctcc cgggcgggaa cgagaagacg cggtttgatc
    26281 atcgccgagt agccggaccc ttcccggtcc catggattcc ggcgcctgct cccattggat
    26341 tcgggcaacg ccgggcggac aatctgcttc gggggcatgg gtgaccgccc agttccccgg
    26401 tgtcgcaatc aagaacggtt ccccgctttg ggaagggtac ccatgaaagc attgagcgtg
    26461 cagcagccgt tcgccttcga gatcctgagc ggacagaaat ccatcgaagt ccggacctgg
    26521 gacacccttc acaggggaga ccttctcatc tgctcttcgg gcagacccgc gtattcccgg
    26581 ggggagatgg aggagctcga ggacgagtac ggctgcatct tccgctacgg cacagccttg
    26641 tgcgtggtcc gagtcgcgga cgtgcgtccc atgagcagag gggatgagga gcgggcgctg
    26701 atggatgaaa tcgacccgga ctcgtattcc tgggtactgg aagaggttcg tcccgtggtt
    26761 cccttcccgg tgaagggaag acaggggctc ttcgaggtcg aggattccct catcgtggtt
    26821 tcaccgttcc ggtacgatca atccgttgtc gtaaagaccg gaacggtggc ccaagacttc
    26881 gggatcgact tttccgggtg gcgggggagg gtttcggatg tcttccccgg cgaagacggg
    26941 gaaccccggg tttttgtcca gtgggacagc ctctctctcg aggcattgcc catcggcgtc
    27001 atcgagacct gcgagcggga aaaggtcgac tggaccgggg ttctcctgcg cttcagcgag
    27061 atcgagcccg cgaaacctcg agatgcctgg gaggatgttt gggatgcagt cgaaaggatc
    27121 tacgaagaga acgctcacct ttttccggat caggattagg attcgaaggt cggatccgag
    27181 gtggctgggt ggtcctttcg gggccccact cgactgaatt cctcaatccc accgccgagg
    27241 cataagaccg gttccgcaaa cccctcggag atcgcttttc ccgcagaccc ccttctccgg
    27301 tctcatgaaa attccggaat gtgtgaacgc cctctcccgg gacgttccct cctttcacgg
    27361 cacccagctt cccgataccc tcggcggact caggccgaag aagccaggaa gaacgcccaa
    27421 acggagaaca ccaggccaag gattcccagg gtgctcatgg tgccgtagaa caaccaccgg
    27481 agccggttct cctggagaac cagggatacc gccccaatca ccaggctgac ctgcaaaaac
    27541 agcagggcca ggtcaaactt gtctcccgcc tgccccaata ccgaaagctt ggcttcccac
    27601 tccttggccc cgacgatctt acccagttcg ccgtcaatgt cctggaccca attctccttg
    27661 ccgaccttgg ccgagcccag tagaagctcc cgcttttcct tcttgtacct tgcaatatcc
    27721 tgctcgagcc ttccgagaag ctgttcgagg gtcgactggt gtgcagggtc gatggacccg
    27781 gcctccatga gggtcttgac cagatctctc tgcccttcca ccaggctctg cttgacgctc
    27841 ttggactgaa accaggcata aatgttcgcc ttctcgtttg tgcccatgat ttcatcgtcc
    27901 ccgtacttgc tcgccccgag gtccgtgatg gccagcaagg cggcaaaaac cgccagcgtc
    27961 aaagcacaga cgatttcaaa gcgcttcttt tctctttcca ggtcctgttc cgcgggcatc
    28021 gcgtccgaca tggttcttcc tccaaggttg agccctacat ggtgtctgaa tgcctttccg
    28081 aacccataga cctgcgggat tccccccgcg caagggtcgg cccttaacta ccatgaggct
    28141 ttgtcgatgt caaaatccat gcgtccctgg tattccggcc ccaaaggctt ctcgttcgtc
    28201 gtgccttgac gtgggttttc ggcgacctaa cctttaagca tgaaacctaa ccgctatgcc
    28261 atggaggtga atcatgagag tcattctgaa gaccctgttg ttggcgatat tgatgtcgag
    28321 tcttgccggg tgtgccaaca tgagcgcccg ggaacagagg atgctgagcg gcggggccat
    28381 tggggccggg acgggtgcgg ccatcggcgc cgtgaccggg ggatctccgg cagtgggagc
    28441 cgcagtgggc ggagcggccg gagttgtggg cggagccatc gtcgacgagc tcgagaatag
    28501 acgataaccg cagactggga tcgaaccctg ggaccgaggg ggaccgtgcg gtaaggaagg
    28561 ccccccgtag aggagagacc cccggaccaa cccggctccc ggtcggtacg gccgggtgga
    28621 ttccgggttc gttgcccatg gaaagcgggt cgtcattccg gcgggctcgg gacttcggtc
    28681 tcggggtcgc cttcatgacg ctgatgatgg accctgtact gaatgggaga agatccgatg
    28741 aagatcggcg gaaagatagt ttattgtctg gcctcattgg cgtttgtggt gatttcggga
    28801 gcggcaatgg gcgctcgggc aggtaccggg atggatcgat tcgacactta cgatttgagt
    28861 tctccgcaaa tcgaacggct cccggacggc aatctcgagt ccccgtatcc tccctactac
    28921 cgcatcaccc cgtaatgtct ggaaccgatg atctcggtct cccatcgaga ctgaggggag
    28981 accgtaccgg acattactcc accggtcaaa accagaggaa atttcccggc tctgccgggg
    29041 agacacccac cgtacccgac tttccgggac gctgcgggga ggagtccctc acagccccgt
    29101 tgttttgccg aaatatcgtg aaggcctgcc ggagaatgcc cctccatgcg ccgttcgcct
    29161 ggggcgaacc actgagtttt gcgaggcccc cggggccgtt gaggatcgca tttaaatttg
    29221 agcgattcgc tgttggtgtt tggctgtgag ttctccggca tgtatattag gatttgcgga
    29281 catctcccgt tgggtggaag ttggaacact tcaaccacaa aaattccaaa actaacggct
    29341 gaacgctcgg tctcgatctc atttgccttc ggtcccagca gtcgggtctc aggcggtcgg
    29401 gtccctcggg gaatttcttc ctcgagggcc cttgcctgca gtaagagcca aacccttgta
    29461 tccaaaaccg aacggtcccg gattggcgga ccgatgggct cccgttactt ccggaaattc
    29521 cccgaacgaa gggaaggaat agggccgccg gctgcacggg agcccctctt tgccctctca
    29581 tttctcttcg aaatcggttg tccatgacgg gaagtcctgg aaatccgtcg ggacggtccc
    29641 ggaaacccgc attgaacctt ttccccggac ttggcattgg atttgctctg ctgagaggca
    29701 aatccggaca gtgagcacgg cctccagcgg cttttgtcat cctgaacctc gggctctccc
    29761 ggagggtcgc acgattcctc tcttgtgctc actgcacagc ccgctgcact gtctcaaacc
    29821 ctgttatgag gctatcggtt ccgtgagaga gggggtgaga attccctgga atccctggga
    29881 aggagtcgac cgctgcctgc catcctcagc catccgtgaa gcaggggggc aggcgccgcg
    29941 tagcctggag accaacgatg gaacgaccac tcatgaaaca acagaagggg gatttatgag
    30001 aaggggaatt ctatgtttta tcgtcgcgct cacgaccctg gggtttgcca accccggagt
    30061 cgctcttgcc ggggggggca cctacgacgc ccacctctat gccatcaccc agtggaccac
    30121 cacgtgctcg gcaggggccc gggacttgtg ggacgacatg ggaaaagcct ggtacaacga
    30181 ggtcacgaat tcgggatttt cctttttcgg gtggtgcctt tggggacatt gcgatgacta
    30241 ctacaacgca gcccggcaat gggtggacgg cgcaatgagc gccgctcctt ttacggaccg
    30301 aaccctcttt ccggcctggg gccaggatac ctcttacgtg gatattgcgg atgcggccat
    30361 gatctgcacc cacggagggg atgacggcgg ttattggcga gggaagatga gaaacaccga
    30421 cgggaacggg gattgttaca tcaatgcaaa cacggaattg agagtggggg attgggacct
    30481 cgagttcctt cacctttcct cctgccacag cctggacgac aacatgatcc ccaacgccta
    30541 ccgggttttc agcagggcag ggtcttcgag gatccttcat cagctggacg gatttcacgg
    30601 gtgcatgtgg atcgggtcga gcttcatcgc cgactacgaa gatttcgccg atgacgcctt
    30661 cgacatcccc atcggcctgt cgtggatgct caacatgtac cggaccaacg tgaacggcca
    30721 gtggactcag tgtcccatcg cctatgccgt agggagcagt gccgacgact gcattaaccg
    30781 gttgaccggt gagcgataca acaacgttta ctcggatccg tcggccgtca attactattg
    30841 ctactactac taccccaact gtgctcccga ctgcgaaacg cccttcgggc aagactggtc
    30901 caattaagct gccgaacaaa ggaggaagca atgtgtctgc gtgtgaagat actctttatg
    30961 gttgcttttg cagtggtcct ggtcatgccg ggggcggcac tcgcccagac cggcggctgt
    31021 gaggcctact tcgctctcat cgaggacaac cttccttcca tggttggaga cgatgcggcc
    31081 ctgaccagtc tcatcacggt gacggccaac gtgtggacgg acaacgtaga cccgacgaag
    31141 atcatgaagt ccctgggaga gcgggaattc tctcagaaag gcctcgagaa gtaccgggtg
    31201 gggctcatga aatcgtcgtc ttccaagatg aagtataacc tcgaaagagg ggagctacgg
    31261 tacgtgaacc aggatcggcg attcgactcc gcgaagccgg gcaaggccat cgatccgtcc
    31321 aagggcctgg aaatggctcg aaagctgttg tcggggaaca tcggggtgcc caggtcggag
    31381 atcctgggga aaaacacctt gtcccaggtg ctccagggag ggatcgccag gccgggcact
    31441 caggacctgg tcaagcgcta cgacattgag agccactttt tcttccaacg ggccatcaac
    31501 ggcattccgg tcatgaactc gggagccttc gtggccgttt ccaacaaagg gcagatctcc
    31561 cagtttcagg tgcgctggcc ggtcatgagg gtggatcagt ctctgctcaa agcgggagta
    31621 ctttctcaaa aggaagtcat caacaacatc tacaaccgcc tggtgcaaga ccagccatgc
    31681 gaccaaacca cgagcatcaa gaagctcaac atgtttatcg cctatgttcc ctcggtggta
    31741 acaaacccgg acaaggacaa gattcccgag cccgcacgcc gggttcgcta cacgccgaag
    31801 ctcatcgtct acttcctccc tgatgcggac ggggaggccg gcgaagttct cgaattcgac
    31861 ctgttcaaca aggacgggag gccggaatca taaagaccgc ctcgcgcgtg ggctccccgg
    31921 aacccacgcg ccgtttagga aacctgaaaa accggcttct tttgcatgcc ggggcgccca
    31981 tcgtccccct gcatcaccag gggcggaaga attgccggag taagcaagga ccctcgcttg
    32041 gtagggttgg acaggtgatt gaccgaacga aggggagcac cgctttcatg ccgtatgtga
    32101 acatcaagat aaccaaggag ggagcgaccc gggagcagaa ggccctgttg attcgggagg
    32161 tgaccaggtt gctccaggag attttgggga aagacccgca aaccaccttt gtgatcctgg
    32221 aagaaattga aacggatcat tggggaattg cgggggagac cgttaccgtt cgcaggaaac
    32281 gctgacgccc caagggggaa gtgtcgccga aaaccccggg ggggcccggc attcttgcgt
    32341 tctccatggg gccgtgatat gaggagtgcg gctcgaggcg gctcacgccc gaatggagga
    32401 gccatggacg ttccttgatt ctcaaagaat tggaggatcg gtcttgcgga gaacctgctc
    32461 ctcgaccgga cagggacggg tcgtctgttt gttcgagccg attgaagatc ttccggatat
    32521 ccctcggaat ggacgcgggg gtcgtccact tggtttgggg ttggaggggg aggtggcgga
    32581 tgccgggacg gtgtgcgctt tcatgggcaa tcatcctcgt cctttctcta tcgggcattt
    32641 cgggatgcgg gtttcccagg cttccggaaa agaagcacga agttcaactc caggaattcg
    32701 cccgatcgga tcggcgatgg acgggggttg cggtttcaca atccgggagg gttttcgtcg
    32761 cctatccccg ctggtccgaa gatactccca tctccgtggg agagcttctt ctctcgggag
    32821 aagtggtgcc ctttccggac ccggagtgga atcgatggaa tcccggtttg tccccggcca
    32881 accatctggt ttgtgcccaa agcgtggtgg tggattcgga ggatttcctg tgggtcctgg
    32941 acccggccaa tccccagttc cagggagtcg tctacggagg cgccaagctg ctcagggtcg
    33001 acctttccac tcacgaggtg gtccggagga tcctctttga cgagtccgtc gctctgcccg
    33061 ccagttacct gaacgatgtt cggatcgaca accggcatgg gtacgcttac ttgacggact
    33121 cgggaaccgg ggccctggtc gtggtcgaac tggcgagcgg caaggcctgg agacgcctgg
    33181 caaaccaccg gtccacccag tccgagaacc tttcgttgac catcggggac aggccctggc
    33241 ttcggggagg caaacctccc cgggttcatg cggacgggat cgcattggac cgtgcggggg
    33301 actacgtcta ctaccaggcc ttgaccggcc gaaccctcta tcgcattccg gcctctttgc
    33361 tccgtgatcc gaacgtgtcc gaggaacaaa cggccaaagg agtggaacgg gtcggggtga
    33421 tttgtgccgc cgacggcatc gagttcggtc gggacgaaaa actctatctc acggacatcg
    33481 aggggtccgg catccgggtt tacgaaccgg gaaaaggaaa gcgcatcctg attcgcgaca
    33541 aggtcctgtc ctggcccgac agcattgccg tcgcaccgga cggttccctg gtggtgtcca
    33601 cctcgcagat tcatctcggc gatcatccgt ccggtcccta caggctgttt cgcataaagc
    33661 cgtgaccaca ctgggcggat gcggcccaac aaggcggctg cgtccacaga cagcgacgat
    33721 tggctcggaa acgaggcccc ggccatgaaa gacttttctt ccctcggcgt cacccgggaa
    33781 caatgcaaag acaccggaat ggcaatggtc cttctgtgtc tgctggggga gtatttcact
    33841 catcgtcccg atctcctctc ggttgccgtg gggctcctgg tcctggacat gatctttccc
    33901 gtcttcttca aacccgtggc cgtaatctgg attcgcctgt cccacgtgtt gggcggggtc
    33961 tcgtcgaaat tactgttgac cctcgtgttt tttcttctgc ttacgcccat ggggttgatc
    34021 cgcagggccc tgggaaagga cccgatgcgg cggaaacaat ggaaacgggg atcttcttcc
    34081 gtattccacc ttcgcaacca tactttcacc gcaaaagacc tcgagactcc ctactgaggc
    34141 cggggaggaa gcaaaggagg accgtggatt tcttgaagga tttctggggg tacttgaggg
    34201 ttcgcaagaa gtactggctg ttgcccgtcc tggtcactct tcttctattt ggagccctaa
    34261 tcgtcttcac cggcggatcc gccatcgccc cgttcattta caccctcttc tgaaaaacga
    34321 cggcgatgag gaaagccatt ttagggatat ccgcctacta tcacgacagc gccgcagcgc
    34381 ttctcgtgga cggagaaatc gtcgcagccg cccaggagga gcgcttttcc cgaaagaagc
    34441 atgacgcgtc ctttccggcc aatgcggtgg agtatgtcct gaaagaaggg ggaatggggc
    34501 tgggggacct cacggcggtt tcgttctacg acaaaccgta tctcaagttc gaacggctgc
    34561 tggagaccta ccacggcttc gctccccaag gcctgaggag ttttcttgcc gccattcccg
    34621 tatggatcaa ggaaaagctc ttcctgcgcg acctgctcaa caaagagctt tcccgcttcg
    34681 gaaaatcacg gccccggctc ctgtttcccg aacatcacct ttcgcatgcc gccagcgcct
    34741 ttttcccttc cccgtttcaa gaagcggcca tcctcaccat cgacggggtg ggggagtggg
    34801 cgaccaccac cattgcccac ggcaaaggca acaccgtcac gatccacttt cagctggatt
    34861 ttcctcattc cctgggactg ctgtactcgg cattcaccca ttactgcggg ttcaaggtga
    34921 acagcggcga atacaagctg atggggctgg ccccctacgg caatcccgat tcgctgcgca
    34981 cccggcggtg gcggcaactc atcttcgagg aactggtcga tcttcgtgag gacggatctt
    35041 tcctcctgaa catggactat ttcgactacg ccacggggct cagcatgtgc cgagatgccc
    35101 gatgggagga gctgttcgaa atccctcgac gccggatgga aggcgagctt gtccgggaat
    35161 atgccgacct ggccctggcc atccagatgg ttacggagga aatcgtggga cgtctggccc
    35221 ggacggcccg acaggtcacc gggtccccct atctcaccat ggcgggaggg gtggctctca
    35281 actgtgtggc caacggcaag ctcctccgct cgggccggtt cgaggatctc tggattcagc
    35341 ccgctgcggg agacgccggg ggcgccctgg gtgccgcgta tgcggcctgg tacatccggg
    35401 agggcgcaga acggagcgtg tcccgagacg gggaaccgat gaagggcgcc tacctgggcc
    35461 ccgaatacgg cctcaaggac ttcaggcgaa cggcgcgaaa acactccgct ccttacgtgg
    35521 aattccccac gctggaagaa atggtggaag cggtggccga ccttctcgcc cggggatgcg
    35581 tggtcggctg gtttcaagga aggatggaat acggacccag ggccttggga aaccgaagta
    35641 tcctgggaga tgcccgggac ctgcagatgc agaagaacct caacctcaag atcaagttcc
    35701 gagaaggatt ccggcccttt gccccgtcgg tgatggaaga ggaccttcag gagtatttcg
    35761 aactggacag gccttccccg tacatgctgc tggtggctcc cgtggcccgg aggcgaagga
    35821 aaccggtccc gaacggagag gccgagctgg aaattctcga gaggctctac catccacgat
    35881 cggacattcc cgcagtcacc cacgtggatt attccgcccg catccaatcc gtatcccggc
    35941 aaaccaacga gacctactgg cggctccttc gtgccttcaa ggagaagacc ggctgcagtg
    36001 tgctcgtcaa caccagcttc aacgtccgcg gggaacccat cgtttgcacg ccggaggatg
    36061 cctaccgatg tttcatgcgt acccagatgg actacctggc catgggaaac ctcctttttg
    36121 ataagaaaca ccaggcgccg atccccgaag acgggagttg gcgccttcaa tactccctcg
    36181 attagaccgg acgacatgac acgagccaaa tccctcatca agtgggtcct caacctggga
    36241 attctcctct acatggtctt tctcattctg cttttcctct cccggggtgc gggctatgat
    36301 tttcagatcg ggttccttcg agtccagctg cgccggccgg gcaatccctt gatgatcctg
    36361 gccgtactga ccctgctccg aattctcctc gagatggaaa agaagaatgc ggttctattc
    36421 gtgttttccc tcctgttttc cttgtccctg gtggaaatgg gattgagggt cctggatctg
    36481 cccatagcca atcagcctac cttgaacaaa tggaaacggc cgtctccttc cctgggatgg
    36541 gagctcatcc ccttcagcaa ggggaaagga cacgaagggg cgtatgtcga aatcaattcc
    36601 cacgggttga gagacgtaga gcgttcatgg gaaaagcccg aaggccaata tcggatcctg
    36661 tgcgtggggg attcgtttac ctatggagtg ggggtcgatt tgaaggatac gtatccgaag
    36721 caaatcgaga acctattgaa ctcaacagga cattccgcgg acgtaatcaa ctccggagtt
    36781 cccggctatg acctctacca ggtgttgacg tatcttcagt ccgtagggac ccgttatgac
    36841 cccgatgcgg ttctgtattt cttctactgc aacgacctgg ctggattgaa gtctgccgat
    36901 gaagtcaaga cctattacga tgcgttggtg aacagcgagg aagaccggga gtccgtctat
    36961 gatttctccg gatcttacct catcaacttc acccgcaatg cctacacgtt cttgtgggca
    37021 aaattccgat tcctttacga tgccgactgg attcggagcc tgcacaaacg aaagaaggtt
    37081 ttctcgaggg agtggatcaa ggtgctggtg ggagactttg acgtggagtt tctcaggaaa
    37141 tccctggctc gcatgaagga tttcaccgat gaacatggag tgaggttcat ggtcgtcttc
    37201 gttccggacg ccgttcagct ccacaatcct tcctggcagg agatcaaccg gatcgtgggg
    37261 caactgtgcc gttcccaggg aaccccgttt gtggatgtga ctccgacttt cgaagagcaa
    37321 ccggatgcgg cggctctcta tctctttccc atggatgccc acaccagccc tcgaggaaac
    37381 gccctccttg cagggcgggt tcgggacgcc atccgggacc tgggctggct ttccaatccc
    37441 tgagccgaaa caagcccggc tctttcacgg caacttttcc caaggaaaca agaccaatgg
    37501 gaaccatccc gattctttcc aggaaccaga gctgcggcga tacgaacttc cgggagtgcg
    37561 gtcttttcca ggctaccttg cgggagcttc cggactttta catgaccgac tactccgtct
    37621 gggggctcct ggtggacaac ccccgggagg tcttgcagct cctggacgaa cacgggttcc
    37681 gggtgattcg caagccgggg gcgatggaag tcgagattga cgggttcacc cgggtgcacg
    37741 agatcgtgag gcttttctcg gcggcgggca ttgccttcga gatggcggac ttggtagacg
    37801 gcgtgtacca gggctgatag gggtcctctc ttctgccggg ttcggcagag aaaagggggt
    37861 cagatctccg aatcgggctt atcctcggga tcctcttcct cgtagtagtc ctccagtccg
    37921 tccgtttccc tctcgggttc ttcctcctga agtcgaacgg cgtttcccgg agccgccgtc
    37981 ctccctgcga ccgggtcttc cggtatccta aggcccgcag tgttttgcag cataggtcac
    38041 ctcccgtgtt gggcaagtcg ggtcgacccg gcgcaatccg cctcgctcgt caaccctccc
    38101 gcggttttca tcggaatttt cctgccggtc cgccctcagg ttgtctcatt ggggcacaga
    38161 ccggaactcg gagagaaagg acatctgcgg cactccggcc tggggtcggc acggtgcggg
    38221 aaggccgtgg gacctcaagg gagcgccccc ctcggcatca ctgcatcttt cgaagcaggt
    38281 cgggaagttc ggagtagttc ctcaagaccc cgtcggggcg aaatccgttc acttctttcc
    38341 cttcaccccg aaaaagaagg aaggacatcc ccgccgtctt ggcgcccacc aagtcctttt
    38401 ggggattgtc tccgatgtag acggaatcct tcggcgagac ccccagccgg tccaatgcca
    38461 ggtgaaaaag ccgtgggtcc ggcttcttga aaccgaatcg ggacgagagt aggatgacct
    38521 tgaagaacct gtccaaaccc agcatccgga tctccggctc cgtaaaaacc cattgggcgt
    38581 cggacacgat ggccattcga taggtcttcc ggagcactgg gagagcttcc aggattccgg
    38641 ggaaaggacc gaaggttcga atggtcaacg atcgaaagag cacggccaca tgaagaaccg
    38701 ttttctcgga cggtttcttc ctgccgaagt gccggagaag gtcctcgaaa acccggaaga
    38761 catccacctc cggatgggct tccttgcttc gttgcaacgc ctcccggacc tgctgcagga
    38821 aggcgttttt gagctcctcg ggctgcacaa acacccggag ataggccagg tgccgggaca
    38881 gagcctcata caccgatgga tcgtgctcat cggtcctaat gtcgatcatg gtgccgtaaa
    38941 tgtcaaacag gagcgccttt ttcatgcagt ttccatcccc acttcaagca ctgaaacgcc
    39001 tctctgctca gtctcctgcg atagtcgcga tccaaataaa ggttcctggc gatcctcaat
    39061 tccgtcaagg ccatgtaaaa cggaatccgg gcggtgattt cgacaaaccc ctcctgcccg
    39121 tgatgtaaac ggtcgcagta actccgcagg aaatgatgga tgaagggctc cgcgccgaac
    39181 gggttccccg tcctccagaa gaaggcgtgt ttcaattctc cgcacaccat ccccaggtcg
    39241 aaggcccggt cggtatcttt cattctttcg aggtcgatgg ccacgatccc gccatcctcg
    39301 gtgaagatga aattggtagg ggtggcatcc ccgtgaacca cgacgttctc ggtcctccga
    39361 aggcagtcac tgttcagcca tcgatccatc agggtccata gggacggcgc ttccgactct
    39421 gggacaacgc cctttgccag gagccggtga aggatttttt ggaaataggc acccacaaac
    39481 ccgaggtcca ctcgactctc ttgcgccgtt ttcgcgtgca gcagggaaag aaagcgggcc
    39541 aattggccaa gagcctcttt cagcggagga tgctctccac ggtagatggc ctttttcaag
    39601 tagtggtcca ggtcgcaccc atccgcaaat tcctccacca gggccagacc gatgccctct
    39661 tcccgggtga tggggcgtgc aaccgagtga ggaaaacggt cgaaccccag atttcgaacc
    39721 ctcaggagat tctcgtactc gcccttgatc ctgagagctc tgtccccgag cgcatcctcc
    39781 aggcgaaaga acttcccgat gatggtaacc cggctctctt cttcccggta ccggtacacc
    39841 agagaggagg acatgcgcca cacatggaac acgggccgcc tgacctgggg acagacccgg
    39901 gtggcaagga cctcgtaaag ggggtcatgg ttgtccaata tccccaagaa cagggctttg
    39961 gtcatgtccg gtccgttccc tcccaccggc tgcaagcaat cgtgcccgca tcctcccgcc
    40021 ggacccgttg cgaggcccga acctccattc tgcggggatt cccggcttgg cgggggtctc
    40081 ggccaaaacc ggaagggatc atccaagggg cccatttcgt gcgtcggaac gatgtgggaa
    40141 gcgcttcggg tcgagagttc cgttcccggt cccgcagtgg aaggggcctg agattgcctc
    40201 ggagaagaag gccccgggtc ctactcatcc ttatgcagtc gacggtgaag aaaacccagg
    40261 tgactccgca ggactcgatg gtgcactcga attttctccc ggcgcaggaa agccgcaatg
    40321 tgctcctcgg cccgggaaag gtctccggga acgaagatct ccattccgtc ccacgtgtag
    40381 gagtgccccc cctgaaccgg aacgaaccga agcttcccac gttccatgag cacctgaact
    40441 tgggctcgct cgatctccgt cattgattca tggtcccttc cagacgaaag gacggcaccc
    40501 cggcgaaaga ccgggccaag gaaagaagtt cctcgcctgc ccaacgcatc agcgtttggc
    40561 cttctttctc gtctcaggct tttgggcaga ggttttcttg ggggaggttt cggttttttg
    40621 ggacttgccc gcagacttcc cggcgctcct cccggaacct gtccgggtta cctgggaggg
    40681 ttgggtcctc gatgtcttac cggttacggc cttggagctc ttggaggcgg tcgggccctt
    40741 ggccttgccg accgtcttgc tcttgcccct atcgacgctc ttgctcttcc cgctgctctt
    40801 gccgaacttc ttgcccttgc tgccgctctt gctcagtttc ctgctcttgg agctgctctt
    40861 gcccttgtgc ttggacctgg aggaagggct cgccttggag tggcggcgag ccggtccgga
    40921 atcctgcttc aagatctgtc gataatcctc gcgaagttgg cgaagggtct catcgtactg
    40981 ctcgagaacg ttttgagccc tggagaccgc ggcccccatc cccagggaaa gggtgaagga
    41041 gagcatgacg gtaaaaagct ttctgaatcc catatctcca aatctacctt ctgtaaggag
    41101 ttgcctgaga cgctcctgac cccgggaaac gtctccccca ccgtccgtgg ataaaccgga
    41161 gacgtgactg taaaccgaac gatgctatct ccctggagat cgatccgatg atacaggcgc
    41221 atccggctcg ggtgctcccc ccccccaagt ccgaattccc agggaatgta ccatatggcc
    41281 gactcttttc aaaaactttt ctggaagcga gggcgtggaa ccggcaagcc ggtgaacccc
    41341 gggtccttgt ccgccctatg ggacttgccc ggctcccccg ttccccctgg ttcaaccctt
    41401 cccccggggc aggctcccgg ctctcgacga ccggggggcc gggaaaccgg tttcggcaag
    41461 agccctcaaa ggggcttcca accctgcatc cggtgaaatt cggtcctttg ttcgtgcttg
    41521 ggaagaaatt ctttaaggga ttctgcgatt cctccgatga tgagactaga cgtccaactc
    41581 gaccaagttg cacaacctgc accggtgggg tgcacaaaca ccacttccag gggggaagac
    41641 atgtttggaa aacgctcgct gagattcaaa ctggtatttc taggagtgct cttatccctt
    41701 gcgccgttgg tgctcatcac cgccaacacc ttcttccaga acaaaaagat tgtaaccgat
    41761 gccggtgcga tcatctccgc tcaggcctat gcggacctgg aacacctggt gaagaacgtc
    41821 catgccatcg tggaaaccca aaacattgtc ctccaggatt cggtgaagaa ctgcatcaac
    41881 gtggcacgca aagtcctttc ggacgcgggg gggatcaggc tttccggtgc ggagaaggtc
    41941 ccgtggaaag ccgtaaacca gttcacgaaa caggaagtca ccgtggaatt gcccaagatg
    42001 tatgtgggcg attcctgggt agggcagaac aaggacatga aaatatcgac cccgatggtg
    42061 gacgacgtca agtccctggt ggggggaacc tgcaccctgt ttcagcggat gaacgatgca
    42121 ggggatattc ttcgggtggc caccaatgtg gagggggcgg acaagacccg ggccatcggg
    42181 acctatatcc ccgccgtgaa tcccgacggg aagccaaacc cggtggtggc cgccttgacc
    42241 aaggggcaga cctattacgg tcgggccatc gtggtcaacg aatggtacat cacggcttac
    42301 gaacctctct acgatgggaa caagcagtgg atcggcgccc tgtatttcgg gatccgacac
    42361 gacagtgttc aggacctccg cagacaactc atggagatca aggtcggaaa gaccggctac
    42421 gtgtatgttc tcgactccaa ggggcactat gtcatctcga agaatgggga aagagacggg
    42481 gaaaacatcc ttgaagtcaa ggatgcccgg ggcaacttct ttatcaagga gatgatcgcc
    42541 aaggcccagg ccgccaaagt cggatcggtt ttcgagcacg aatacccgtg gaggaacccc
    42601 ggggagcagg cggacaagca aaaggtggca aagctgatct attttcaacc atgggactgg
    42661 gtcatagggg cgggtaccta tgtggatgag ttttttgaag cccgggacat gatcgtgacc
    42721 tcgggcaatc ggaaccttct caccgggatg atcctggccc ttgcggccgc ccttgccgcg
    42781 gcgttgatct ggtttttcat ctcgcgcaat ctttccaacc gtctttcact tttcacgaat
    42841 cagattcagc actgtgccca ggaggtgagt gccgtttccc accaggtttc cacatccagc
    42901 caggcccttg ccgaaggatc ttcgcaacag gcatcctcca tcgaagagac ctccgcctcc
    42961 ctggaggaga tgtccaccat gacccgtcag aatgcggaca acgcgggcca ggcgaatcgt
    43021 ctcatggacg aaaccaacga ggtggtttcc gttgcggcaa cggggatgca ggatctcacg
    43081 gtttccatga acgagatttc cagggctagc gacgagactc agaagatcgt gaaaaccatc
    43141 gacgacattg cctttcaaac gaacctgctc gccctcaatg ccgcggtcga ggcggctcgt
    43201 gccggggagg cgggagcggg gttcgccgtg gtggccgagg aggtccggaa cctggccatg
    43261 cgggcggcca acgccgccaa ggagacctcg gccctcatcg aaggcacagg ccgtcaggtc
    43321 cgggcgggct ccgaggtggt gggacgaacg gcggaggcct tcaagaaggt ggctgcgtcg
    43381 gcctccaaag tgagagagct cgtggccgac attgcgtccg cctcttcgga acaggcccag
    43441 ggcgtcgatc aaatcaacgc ggcggtatcc agcatggaca aggtcgttca agagagtgcc
    43501 gccagggctg aagaatccgc ttcggcctcc atccagatga gtgcccaggc ccaggagctg
    43561 acgcaaatgg ccgacgagct cgcccatctc attgccggaa gcaaagtctt tcagagagag
    43621 gtcaggaacc aggctcccga cgcgagatcg gaaaccaacg gcaaaccgcc caaggaattc
    43681 cgtaggacgc aggctctgca acaggcggaa cgcctcagga tttcccaggt tcttcccctg
    43741 gaccctgagg aggaggcggc tccgccccag cccaggagaa aagtcacgaa ctcgggaccc
    43801 aaacaggcga ggatcgggtc cttctaagcc gggaaggcct tgtctgagcg gcggaggcac
    43861 cggcaaagcc gttaccgctt cacatcccat tcgaggaaag aaagcccacg ggcggttcca
    43921 cccgctggaa ccgcccgtgg tttgttttcg aggggggcaa ccatccggca aacacaggca
    43981 cggtcttccc gatttgtttt ggacagggag gggagatttg aggtaatcaa caagaggtcg
    44041 gggtagtgct cccgtatgcc gaagatgcgg ggacatatcg ggaggcggtt gctccgtgcc
    44101 tcgtccctct ggagacacga aactcgatag ctttgaagaa gcggcatacc gaaccatgct
    44161 cggaggattc aacaaacggg tgcccactct ttccacccgc cctggaatgt cgggcttgtg
    44221 agaacgccat gacggcagtt ttgttacaga ccctggcttc cgattacttt cgccggctgt
    44281 cgcgccgctt cccggtcatg tgtgccgggg atgaattcca tgcgtttccc cgagccctcg
    44341 ccgccaatga attcttggac cgaatggagc agctgagtcc cgaggaaatc gaggagagca
    44401 ttgccgtact gaaggaattt cagcggcagt tcgacgcgcc tttgtatccc gaggaggggt
    44461 tcgaggagag cctggatcga cagctgctga gtgccggcat cgcgggcatc ctgctggaac
    44521 tggagtcttc cgaatcctgg cgtcacgacc cgtccctgta tctcaagatt gcctttgtcg
    44581 gcctggatca cgccctgacc aagccctgcg atgattcgtc ccaggtagcc tggagaacct
    44641 tggcccggct ggataagatc ccctcgctgc ttcgccgggg agccgaaaat ctccgtcttg
    44701 tccctcgaac gcaccatcgg gcggctctgc tcatggtccg agactgccgg gaatacctgt
    44761 ccgagcttgc cggaattgcg tggttcgagt ctccgagcag gtattccggg gccctgcagg
    44821 aggtgcgcac ctccctcgag gatttcgaga aagccctcaa cgacctggcc ccgtgcgagg
    44881 acgaccggat ttgcgccccg aagctggaaa cggtcctggc tcatcgattc ctttgggacc
    44941 ggtccatcga ggaagtccgg gaaatcgggg aactagagtg ggaggacagc ctcaacgagt
    45001 tggaacgatt gcgccgggag atcaacccgg ggcaatcctg gcaggagatt tatcattcct
    45061 atctgccccc ggaagtccga tccgtggagg tcttcaccct ttacgaacgg gagatgggcc
    45121 gtctcttttc cttctttacc cgtcacggat tgggggaatc tctttcggaa tcgttgcctc
    45181 tcatccggga aaccccacga tacctccgtt cccttcgcgg ttccgggtct tttgctgccg
    45241 cactgggcgc ggacccccga gaaaaggact tcttctacct caccacccgg cctttggcac
    45301 ggcaaagtgc cgagtctcgg gagtcccttg tcgcaaggct ccatcgcgaa tacgcttttc
    45361 tcgccgccca cgagaccgtg cccgggcatt ttctcctgga ttccgtgcgc agaaagatcc
    45421 cgaaccccat ccgccgccag atggaatccc ctctctttta cgagggctgg gcgacctatg
    45481 cggaaaggct tctcggggac tacggatatg tacaaggtcc catggattgt ctggtgcatg
    45541 cccggcgccg cctttggagg gcggcccgat gccgtatcga tgtggggctc gcaacaggtt
    45601 ccctgggcaa gggggaggcc gttgccttgc tggtgcgcac gggaatttcc gccgaggacg
    45661 ccgcccgcca ggtccgtcga tttcagctga gccccggtta tcaactgtgc tacactctgg
    45721 gttgtcatga aatcttgcgc ctgaggagcg ctcatggaaa tcgactgggt ctgaaccgtt
    45781 tccaccgcga aatcctggaa gggggacaac tgccctttgg gattatcgac caacggctgg
    45841 aaaagatcgg gacctttcaa gaagaacccg gcaggaggaa ttctcgttga gatcgtttct
    45901 gggataccat tcggagtgga acctgggaag tcccggcggg tgggactatc agcgggtaac
    45961 gcagatcatc ggaaaggccg tgtggcaaag gattcaacaa attcggcccg tttccgtcga
    46021 tctggacttt gaccatccgt tccttttccc cgtgtatggg tttgtggaaa tgctcgtcga
    46081 ggcccaccgg tcgcgctcgg gaaatgcgcc cggcctgatt gcggtcgtcg ccgaagaaga
    46141 aaccctcgag gacgtgacgg aaaacatcaa ccttgcccgg cacctcgacc gcgtcgacgg
    46201 catcgagggg gctctcctgg cgccccaggc ccttgaactc aagaacgggg tcgtgtgctg
    46261 tcgaggcaag gcggtctccc tggctttcgt ggatttcaat tccgacgttc tcctctcgtt
    46321 gcaccgcaag cacgatctca ccccgctgtt gcaactggtg cgcgagggca gggtgatcaa
    46381 cccccggggc acggaaccca tcaatgggaa aagcatgttc gaagtcctca cgggggacga
    46441 ctccgggagg tttcacccgg aaatcactcg gcgaactccg tggactcgcc ggttctaccc
    46501 ccggagcacc cggggacccc ggggccagag gatcgacgac ctgatcgact ggacccggga
    46561 gcattggatc gacctggttt tgaaacccga gcgggggtat tcgggcaagg gggtgagggt
    46621 aggggagaca catcccgacg cggaggaggc catccgcttg gccctggcgg acggcagcta
    46681 cattgtccag gaaaagatct cccttcctct gtgggccgag gagattccga cactgcgccg
    46741 ggaagacgaa accatccggc tggagcgcta ccagactgac tttcgatgcc tcatagggca
    46801 tcaaggactc ctgggattcc tgggccgata cgggggagtg cccaccaacg tgggcagcgg
    46861 cggcggggtg cagcctctcg cggtcctccg ttcgcccatg agtgtacggg atgcggtgga
    46921 gcgtgcgaac gaagcggttc tatccatgga ttacggggat ctcgaggaag tgctccgcct
    46981 gcaggaacgg atggccgttg aacaccgttt tacatacctt ttgggtccca tcaagatcgc
    47041 tcttcgaccc cggatcattc ggccggagca gatcgaggcc ctggccgggt attgcgaggc
    47101 catgtggctg gattgtctcg aactcgagaa gatgtggctt tcgggcgaac tggacgaaat
    47161 catccgcatc gagcccgagg agttggaaat cgcccggatg cagccgtgga gaggtcaacc
    47221 ggctatcatc gcctcggacg ggcttttcag cttcggggct cacccggaga tgccatgacc
    47281 gtgaccgtgg atcccgactg gtggaaaacc ctgtttgacg agatctacct gctcactgac
    47341 gctcgttccg tgtgtgacgg ggagatcacc cgcagggaaa tcgacctcat ttgcgggatt
    47401 cttcccatgc ggccggaaca cagggtcctc gatctatgcg gcgggcacgg tcgacacagc
    47461 ctggagctgt gttcccgggg ataccggggt tgcgtcttgc tggactattc cccgtctctc
    47521 ctttccgtgg cccaggcgga ggcccttgcc cgggacctgc cggttcggat ccttcgggcg
    47581 gatgcccgca acacggccct ggtttccgaa agcttcgacc atgtgctcat cctggggaac
    47641 tccttggggt acgccgggga cgaccgggcg gaccgtcagg tcctcgggga ggccttccgg
    47701 gtgctgcggc ccggcggatg ggtcctgctg gaccttgcgg acggagactc ccttagggac
    47761 tcgttcaatc ccgagggatg gtttgagatc ggcggggact gggtggtgtg ccgccgccgt
    47821 cacatggagg gggaccgggt gagtgtgcgt gaagtggtgt tgagtaaaga aaaagggctg
    47881 gttcgggacc ggagctattc catacgtttc cacgacgccc ggtccgcagc cgcactcctg
    47941 gtggatatgg ggtttgccga cgtgcaggtc gttcgggact tcgtgctccg ggaggagcgc
    48001 ggcgactacg gtttcatgag gcaaaggatg ctggcggtgg ggcaaaaaac gggaaagcct
    48061 taccgggccg ggggcgggct cgtcggcgga tgacaagccc gagtcctgag ccctgagagc
    48121 tccccgtcgg ggtgacatga accgggaaga aaggagtggg ttttcatgaa aaaactggtg
    48181 ttgttgcgcc atggagagag cctttggaac agggaaaacc gtttcaccgg ctggacggac
    48241 ataggacttt ccccgaaggg gatcgaggag gccgtcgagg ccgggagggt catgaagaag
    48301 gaaggccttg tcttcgatgt ggcttttacc tccgttctcg cgcgagccat caagaccctg
    48361 tggcttgccc tcgaggaaat ggacctcatg tggattccca tccggaacca ctggcgcctc
    48421 aacgaaagac actacggagc actccagggg ttgaacaagg tggaaatggt ggaacttcac
    48481 ggaatggaac aggtgcagat ctggaggcgc agctacgacg ttcctcctcc ggccctgacc
    48541 ccggaggacc cgcgcaatcc cgccaacgat ccccggtacg cctccctggc ccccggagag
    48601 gttcccctgt cggaatgcct gaaggacacc gtggcccgtt tcctgcccta ctggcatgac
    48661 accatcgccc cggtcgtcgc ccaggggaag cgggtgctca tcgtcgccca cggcaacagc
    48721 ctccgagccc tggtgaaata cctggacaac gtttccgacc gcgagatcgt gggtttgaac
    48781 atacccacgg ggatccccct ggtctacgag ctggacgacg actgcaaggc cgttcggcat
    48841 tactacttgg gggatgagga ggccgttaag aaggccaccc aggccgtggc cgatcagctg
    48901 agaaaaaacg cctgagagac ttcttcacac ggtagcggac cacccgaggg cgatttctcg
    48961 gccggttcct tccgtcctcc cgacccgcgg cgggtcccgg acctgcgccg ggggccgggg
    49021 gtccggtttc tgcacaaccg aggaaactcc ggtcgtccaa agcgtttccc gggagggacc
    49081 cttcgggcgg gtcgcggatc ctggaagttc ttctgctgcc cgcaaaggtc cgagacccgg
    49141 ggagcaggaa gcgagggaag catggttttg gatcgcgtgg atcccacccg gagcagggcc
    49201 tggcaaaggc ttaaggatca ttaccaggtc atgcggacca ggcacatgaa cgatcttttc
    49261 gccgaggacc cggatcggtt ccgacggttc agtttccgat tcggcgacat gatcgtcgat
    49321 acgtccaaga acctcatcac cgaagagacc atgaatcttc tcacggactt ggctcgggaa
    49381 tgccgggtca aggagtcggc cgagaagatg ttttccggag aaaagatcaa cgaaacggaa
    49441 gaccgcgcgg tccttcatgt ggctctgcga aatcgttccg gtctccctat ctgcgtggac
    49501 ggaaaggacg taatgccgga ggtcgaggaa gtcctcgaac agatggaggg gttttcccgg
    49561 agggttttga ccggcgggtg gacgggattt acgggaaagc ccatccggga tattgtgaat
    49621 atcgggatcg gcgggtccga tctggggccc ctcatggtta ccgaggccct gagaccgtac
    49681 tggcagcccg gcatcacgcc tcatttcgtc tcgaacatcg atgggaccca catcgtcgag
    49741 aacctcaaga acctctctcc ggagaccact cttttcctga tcgcctccaa aacgttcacg
    49801 acccaggaaa ccatgaccaa cgcccacacc gccaggaagt ggttcctgga gcggggggga
    49861 gaggaatccg ccgttgcccg gcactttgtt gccatctcca ccaatgaaaa ggaagtcgtc
    49921 cggttcggca tcgatccttc gaacatgttt cgcttttggg actgggtcgg gggacggtac
    49981 tctctttggt ccgccatcgg gctgtccatt gcctgcacca tcggcttcga ccatttctcc
    50041 cagttgttgc agggggccca cgtcatggac ctccacttcc gggaagcccc cctggaaaaa
    50101 aacattcccg tcgtgctggc cctcctgggg atctggtacg gcaatttctt cggggccgag
    50161 acggaagcga tcctccccta cgatcagtat ctccaccgtt tccccgctta cttccaacag
    50221 gggaacatgg aaagcaacgg gaaatccgtc gaccgacggg ggcggacggt gacctatcag
    50281 acgggccccg tcctgtgggg agaaccgggc accaacggac agcatgcttt ctaccagttg
    50341 atccatcaag ggactcggct ggtgccctgt gacttcatcg ccccggccgt cagtcacaat
    50401 cccgtgggag atcatcatcg gatcctcctg tccaatttct ttgcacaaac ccaggccctc
    50461 atgcggggga agtcccggga agaagtgcag gaggagatga ggaaggccgg gatgtccgaa
    50521 aaggaagtgc agagactggc ccccttcaag accttccccg gcaatcgacc caccaacagc
    50581 attttgatcc gaaagttgac ccccttcacc ttggggagcc tcattgccat gtacgagcac
    50641 aagatcttta cccaggggat cgtctggaac ctgttcagtt tcgaccaatg gggagtggaa
    50701 ttgggcaagc agcttgccgg acggatcttg gcggacctaa tggaagattc ccccacggag
    50761 gctcacgaca gctccaccaa cgggctgatc aacctctaca aggaatggag atgagcctcc
    50821 cgggaggtgg aacccaaccc ggacaggaac ggagaacgcc ctatggaagc acatgaac
//
LOCUS       DAOOVC010000011        50824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_420_length_50824_cov_6.620280, whole genome shotgun sequence.
ACCESSION   DAOOVC010000011 DAOOVC010000000
VERSION     DAOOVC010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 50824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50824
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_50824_cov_6.620280"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLM79_02805"
     CDS             <1..202
                     /locus_tag="PLM79_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HOV85262.1"
                     /translation="SLVGKPLIHSGIREETQCSVIAVKTGEAMEINPDPRQPIPPDAH
                     LILIGTTEGERRFADRYPEDHG"
     gene            complement(244..1653)
                     /locus_tag="PLM79_02810"
     CDS             complement(244..1653)
                     /locus_tag="PLM79_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 17 protein"
                     /protein_id="HOV85263.1"
                     /translation="MKVAELCVSIAVLVSVFTWGLPVGRVAAAGLPNFVAVNYGPFHK
                     DGQRSGTPIPDSQFLTDLGIITKKFKYIKTYGLDSASRLDRLVPLAGSNFPQLKVFLG
                     VFEDGPHHAAVTRPQLDMAIHQANTYPNTVQCVVVGNECLPGDSTDVPVSVNQLIADL
                     QYVRKAIANKNIPVTTCLAYGAARKYGKQLMPYCDIMMVNIYPFYGGVDISGAWNNLA
                     GAYRDFSGQFPGKQMVVGETGWPSIGPKNGAAVPSIPNEQTCITQILSRAPQLGPVFL
                     FEAFDEPWKSENQWAPHWGIWDKTGAPKINLGSFLTRDAVSIQDRSGNGIEEMALLRH
                     DLDKREIEVHIVHDDAPPPAAATTIRFFGSGWTPVGMVALEDLNANGSQDLAVLALHE
                     KTGVVRVVVRDSATGRLISKIDFDPRFEPKELMVRGERHLAVLGTNSNTRQSQVEVRE
                     ALSGELIKRFRVSDGRFEN"
     gene            2026..3462
                     /locus_tag="PLM79_02815"
     CDS             2026..3462
                     /locus_tag="PLM79_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOV85264.1"
                     /translation="MERNDEAKGAEEGSPRENASMTRRALLKTGLGLGALASMGGVAP
                     DCLPGNPGAAGNAGRLGPAGASHWFEGFLFKDESLVFELIRVLSKMTSRGADLGESLS
                     AAMRIREKEGDTRALLQAWYEEWRKLAERVEKIGDRCLAGGHRVSARDAYFRASEYYR
                     SADFYLHDDPRNPAILELWNQMESCFEKARKLSRPSFETVGIPYEGTRLPAYFRPAGV
                     KGRRAPTLIVHQGFDGTIQETFLAYGNDALERGYNCLVFEGPGQGSVIRKQGLPFRHD
                     WEKVVTPVVDYTLKRKEVDPKRIALMGLSMGGYLAPRAAAFEPRLAACIANPGVLDFW
                     AKTYEKSGLKRNEFKEFLESAPEEYDKISRDLASRSITFYWAVTHGMWVFGAKTPAEL
                     ALKQAAWTLEDCASSISCPTLVIDSEGEQFDPGQAKKLYEALRCPKTYLLFKAGEGAE
                     LHCQAGGALLGTQQIFDWLDETFKRVKH"
     gene            3677..4174
                     /locus_tag="PLM79_02820"
                     /pseudo
     CDS             3677..4174
                     /locus_tag="PLM79_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            complement(4224..5108)
                     /locus_tag="PLM79_02825"
     CDS             complement(4224..5108)
                     /locus_tag="PLM79_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HOV85265.1"
                     /translation="MREVHLPRSLEELWPLLDRRPRAALYAGGTDLLVRMGRGMENPA
                     ALVCLERIEELKGVNEHGDHIHIGSCTTHSRLLAHPLVQRHLPVLAQAVKTLGSPLIR
                     NMGTIGGNLCTASPAGDTLPPLYVLEAEVELRSGGRVRRMPVREFIRGPGVTCIEEGE
                     ILTGIRVKKPARDLLHHFEKVGRRNALACAVVSLAALIGFSPDGVVRRAALAWGSVGP
                     TVVRCPEAEEALLGQRLSADTLQRAAALVREAVSPISDLRADEHYRRRVSGNLLLRLA
                     ERPRPGSSGIPPGCCSSK"
     gene            complement(5105..5581)
                     /locus_tag="PLM79_02830"
     CDS             complement(5105..5581)
                     /locus_tag="PLM79_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOV85266.1"
                     /translation="MEIRFVLNGRNTLVETSPDRKVTDLLREDLGLTGVKEGCGAGEC
                     GACTILVDGESRLSCLMLAAQIEGRHVTTIEGLAQGEDLHPVQEAFVEHGAVQCGFCT
                     PGMVLSAVHLIENLSGPTREEIREGLSGNLCRCTGYTMIVDAVEAAAAKMRGEGSS"
     gene            complement(5583..6059)
                     /locus_tag="PLM79_02835"
     CDS             complement(5583..6059)
                     /locus_tag="PLM79_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV85267.1"
                     /translation="MAYVEVDELTGRIEVKEYLAVTDGGRRINPQGFDQQVQGAVAQG
                     IGYGLMEEVLLEKGRILNPSFTDYLIPTSLDVPDIRSLAVETVESTGPFGMKGIGEVG
                     TNAPLPAVAGAVEDALGSCPGVAPLDPGRVLRAVENREGAAGTAAAARRSRTHKEE"
     gene            complement(6116..7909)
                     /locus_tag="PLM79_02840"
     CDS             complement(6116..7909)
                     /locus_tag="PLM79_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOV85268.1"
                     /translation="MKDRVFGVGERSPRHDAAAKVTGREKYALDHYEDNLLWAGVKRA
                     RIAHGRIRGIDSAEARGLPGVHAVLTARDIRGSNRQGIVHKDQPVLAEDKVRHWAEPV
                     AMVLTEDRETLRAALDLIRVEFEPLPGVFDPEEALHLEAPRVHEKGNLLLEAVIRVGN
                     ATTSLDECHVVVEGDFRVPAQEHAFLETENGIARQQPDGKIVLIVSTQAPFRDRFEIA
                     HALGIEADAVRIIAPYLGGGFGGKDGTTVQCLLVLAAMHAGGRPVKMWWEREESFAAG
                     YKRHPVQMHYRLGALADGTLHALHCRLVYDTGAYAHLGGEVMALGMEHAGGPYRIPNT
                     LIEGRCVYTNHPVSGAMRGFGVCQVTFAIERMMDRLAEKLGMDRLEIRLKNALRRGDT
                     NTSGVTLVHSTGIAQCLETLGRHPLWTRRRKWKDSPGPFKRRGVGIAAAWNAMGYGRG
                     LPDSAIARVELTREGRIRVYSGVSDMGQGNASAFVQIAGEILCQEASHMELVQPDTDR
                     THPSGSSSAGRTTYTYGNALIEACRELKAKLLHRAALMLLLDEPRGLALLPGKVRHLP
                     SGREVPLDALARVFPESDRVVVSQFIMPGPG"
     gene            complement(7926..8402)
                     /locus_tag="PLM79_02845"
     CDS             complement(7926..8402)
                     /locus_tag="PLM79_02845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85269.1"
                     /translation="MASFFTVSALLMIAVRLLAGRFMDRMDKTRALRIFILLLIPCYL
                     LFGRMDSPGTLGIMAACYGLCIGFLMPLLNAALFLASPPTLRGLNTNLALFAMDAGFF
                     LSPYAGGAFLAAGGSFDLLFALCAAVLSMAFLLLIPLKPYGTKTSGKAAAGADPPT"
     gene            complement(8435..9148)
                     /locus_tag="PLM79_02850"
     CDS             complement(8435..9148)
                     /locus_tag="PLM79_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85270.1"
                     /translation="MTDTGKLFSFEFVSLNLVAFFAFCNMSVFYSFFTYLERIGIAPE
                     WRGFLIGLEPMAAFALRLAVVPVLHAGNAATGMLIALTMIVAALFSYGWVVTIPGLIV
                     LRIFHGAAFVLLVSACMSLAVCFIPRVRSAQGFGVLSVSILLPYAVVPPVTEALLRHW
                     QSEQDIYRSVTVLAIPAFAVLRVLRGRLGKVLCSLEGALTRRPTPEEIRLDLKERPVV
                     LLLAVNLLVYFSTPPSSSL"
     gene            complement(9145..10278)
                     /gene="hisC"
                     /locus_tag="PLM79_02855"
     CDS             complement(9145..10278)
                     /gene="hisC"
                     /locus_tag="PLM79_02855"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV85271.1"
                     /translation="MDGFSPDILVPEHIKAFSAYIPSKPDDVLKQLYGCTRIHRLNNN
                     ENALGPPGTACAAIMGFPAVQGSVYPSGDAYHLRGKLAQRFGMHPDQFLAGNGANEVI
                     GFVIKAFCREGDNIITADKTFAVYEWVAEFSGIEARLTPLVDHGFDVSGMLERIDPRT
                     KILFVCNPNNPTGTWWNRETLCGFLDRVGGSALVVVDEAYREFVEDPDFPDGLALVPR
                     YPNLVVFRTFSKMYGLAGLRIGYLAGSREVVDIIRRTCIVYSVNSLAQVAALAALDDE
                     EHVERTLEMVRRGRSFLHRELNGLGLPVISGEGNFLMVRLPMSDSLAYRKLMTRGVMV
                     RAMTGFRFPNWIRVTISQMEAMEAFVEALSSILAEPAQEQGRP"
     gene            complement(10278..11600)
                     /locus_tag="PLM79_02860"
     CDS             complement(10278..11600)
                     /locus_tag="PLM79_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85272.1"
                     /translation="MRRDAAEDVFLTVGARKGFLPLVTGFVEQASLCLGLGREEALGL
                     TLGAEEVFSHLCSVATSTSGPVDIRCSSGGYCVRTEFLLRTDAFDLRSFNLTASVSVE
                     NEADLDRLGLILASRTVDRLDLGRENPRELRLTLIKEKSYPCFREPVSAAAHPLTTYV
                     LRPPTPEEVQFVSLLAGSYYDRHFLSDFLLSPGRLVDMIAVGEYQAVAAVGPAGEIGG
                     AIFWHRTGARTVECFGPYVFNQPPESRMPQGLVEGCIATLARTRAVGLVSMHPTPQFP
                     VGEFEPLGVLTSYAQDGSSLPLRAYFRLLEEDTGSAVWVHPELMEFVETECRRLVLPR
                     EIRPFHNVGETLPRHSVLSAAFDRTQSRAILRPLWPGADADENIAQHARLLREEGIRN
                     IRFVLDLGVGWQAEFTPGLLRHGFRPCLLLPYAGEGDVVLFQLPGECP"
     gene            12001..12678
                     /locus_tag="PLM79_02865"
     CDS             12001..12678
                     /locus_tag="PLM79_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85273.1"
                     /translation="MVPVPVFPCVVQGPKAPCPTEDVGTMVAFCFNDRARRTEYREVT
                     VNVSKEGGVTVSYELPGRIAAFHLNEGGRRKLAELLRKGITLCRTVRESELEMHRVLG
                     VTGEGVKVSFYSSKEGKDTSVEVAWVFFTPDETEKRSVPAPVPAPVERQDGSKGKRVP
                     PSKQENPQKSPEAEPAVPGQTPVMRLGALVLAKEDAEGLLNLLDRVPAAAKAVNAPRY
                     RDDPTAD"
     gene            12928..13644
                     /locus_tag="PLM79_02870"
     CDS             12928..13644
                     /locus_tag="PLM79_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HOV85274.1"
                     /translation="MKRALSVFGVLGLFVLLEIVGGGWSAALSREGGGTPPPPKKAPA
                     VLYVADFEIDSEDVKSDPGILGNRSGRRVGVLNREGPLQRSKDPEIAAPKLRNLLAES
                     LVQDLAAHSLTAVRLPAGRPLPREGWLIRGEFLEVDQGNRVKRAVIGFGTGATDMHVE
                     STVTDLGGDVRQPFLVIGVGAGSGKSPGAVVTMNPYAAAAKFVLSKHASEKDVRSVAS
                     GITWEILQYMKNNGLISARP"
     gene            13917..14507
                     /locus_tag="PLM79_02875"
     CDS             13917..14507
                     /locus_tag="PLM79_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV85275.1"
                     /translation="MDGSMQQEKDADLIRAFLGGDEAAFDALVLAYQDRVYTLCRRLL
                     GDREEARDSAQETFVKVYRALGGFRFDAAFSTWLYTIAVNTCKNKLKSHEYRWWKRRL
                     RLDWDSGSGGESPHREPRDPAPSALARLIQRGDEMRVQKAMDRLPAESRAVLVLRHAE
                     GLSYEDIVRITGLNLGTVKSRLARARQQLQEKLKES"
     gene            14603..15721
                     /locus_tag="PLM79_02880"
     CDS             14603..15721
                     /locus_tag="PLM79_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOV85276.1"
                     /translation="MDCTDVRRRMYEQGDDPMDRRLREEMETHLSRCAPCAREWEDLR
                     KNFETLGSLPTIPAPPGFLRAVREQIGKPSRAGRVKEWWTKPWKIGMPRELAGLVAAS
                     LLVVLLYHGYQRIPAVPGKPEVGAGSLAPSPVPLQAPTAPPPSSVLSLPEKKELPGDV
                     QGMKGAPETEVLRLNVVLRVAGPSKKPDSPALAPAEAVGRADLAGEAPASGSGKTFAF
                     REKAARKAPAPGASRQRPGAPTPVPEAALEAKSAPGKGPSKDRIEDAVAGPPAEAQSG
                     PSEPGPNRAGLPSRDGEDPAARIEEWVLDAGGRVIEAGSRESGKNGRIIRAEVPVENY
                     LFLVNNLSRLGDLDPPPVHPARGKDRETVLVVIQLLFP"
     gene            15856..18303
                     /locus_tag="PLM79_02885"
     CDS             15856..18303
                     /locus_tag="PLM79_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HOV85277.1"
                     /translation="MDRAGRFKGKKAWRLLPVLMVSVGFLWGKGIPADARRTPIQAES
                     AGTGTAATEEDKTLSPYFFVKSEDPAVDPLPLKATRTAVQIAGVIAEVTVVQVYRNEG
                     TRPIEAVYVFPASTRAAVYGMKMTVGERVIEAEIQKREEARKAYEEARQQGKTASLLE
                     QHRPNVFQMNVANILPGDEIRTELRYTELLTRTDGVYEFVYPTVVGPRYSNEPESGAP
                     PSEKWSQNPYLHQGEAPPYEFDIQVRLDAGMPIREMTCPSHGTQVRYEGPNRAQVSLG
                     PAEKHGGNRDFILKYRLSGKEVEPGLLLYEGKEENFFLLLVEPPQAPPETMIPPRETV
                     FIVDVSGSMHGFPLETAKGLLKDLLGGLRPRDVFNVLLFSGGSQLMAERSLPATPENL
                     RNALELIGRQRGGGGTELLPALERAMKLPAHEGMARTLVVVTDGFVRVEPEVFDLIRN
                     SLGQANVFPFGIGSSVNRHLIEGMARIGMGEPFIVTKPEEAPRQAERFRRLISTPLLT
                     RLDFKTDGFSADEVEPPRLPDVFAQRPVIVFGKWKGSPRGTISLHGTLGTQTYDRRLD
                     VAAYKPSASNLALKYLWARSRITRLSDYNRLLASDRRTGEITDLGLRYSLLTSYTSFV
                     AVDSEVRRKEGESATVRQPLPLPEGVSDYAVGGGAPAGIAPMSMAVAPLPSRGLRQGV
                     EGMSEDARGVREAKEVSGVKMKKGDIEARVRVQSLTVGPGLNESQVRSVVEASEKGLL
                     DCLRTLLSREVPEWKVKLTWVVDKKGRVRDLKITTPAGMTREIEACLQGQILAWRFVN
                     RGGTRASAVTLELSVRP"
     gene            complement(18553..20586)
                     /locus_tag="PLM79_02890"
     CDS             complement(18553..20586)
                     /locus_tag="PLM79_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV85278.1"
                     /translation="MFRRFMSAGLRLKVMGGLLLSLIPMVAIVGINYHFARKAALANS
                     RGLMELVCEGGAKEIDGMLSARAASFNSWIGGSSGGWSASDRFGMALEFHTYREVQGQ
                     FETFLKAQDGFSLLVLTDLQGKVEVAAAPSHLDAKVAESLKGTVLKEASALAAGDPRS
                     VALVQNDLLEQLGGKSSRALLFAHRVSGMSGTPNGYFLAYADWAPVQDLLRVSVERLG
                     KEGLRNATAAVLNTADFIFASHSAENQTGKTLDADSDLQSWFKGESGGTVRNFSLNRE
                     DTHLIYLPVRDFSQMAGNAGADEKRSQLCLAVFVPNSAIMKEVREILGLSSGIGILGA
                     VLVLLVGLWISRIISRPLNRVIEGLTESARQVAAGSRQIASVSRQLSQGSSQQAASLE
                     ETSSSLEQMSSMTRQNADHAQQASRLMKEASRIVERAKGSMERLTDSIHDISRASLET
                     QKIIKTIDEIAFQTNLLALNAAVEAARAGEAGAGFAVVADEVRNLAIRSAEAAKSTAT
                     LIEGTVKKVKDGSRLVEETDAEFRAVASTVAQSNEIVGSIAAASHEQATGITQVTNAV
                     AEMDKVTQQNAATAEESASASQELNGQVERTKEFIDELVSLVGGRGGGQDPPVPLEES
                     GSSPRLPGSRGRRSLQAPNPRQGEMVPASSPRTEAREIRPDQLIPLDDDLSNF"
     gene            complement(20752..22524)
                     /locus_tag="PLM79_02895"
     CDS             complement(20752..22524)
                     /locus_tag="PLM79_02895"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV85279.1"
                     /translation="MFSGKTEKTTSIGRTMLWVMCAIFVATTCVLSVFFYYQTQSYAI
                     GEAEKKIKNLLLEHQALHAYIAKHQKPAIGKLKEEGKLPKEYFSPEILSSSYITRNLH
                     EYYNDLRKKSQLPEFYYKLAANNPRNPLNKSDEFERDLIRRFNTGDMTEYKQVIEKEG
                     NRYLYYALPFTKNTQDCMGCHSTPDVAPKELLDRYGDKSGFGEKIGETRAIISIRAPL
                     DTEIQQANKTFLAIAGVLFGITLVLFSGGGLFLRRAITRPIHQVTVGLMDGADQLSMA
                     SRQAAAMSRQLAEGSSLQAATLQETSSSLEEISSMTRQNADNATCANQLMSDAGRIIH
                     QANDSMATLTCSMGEISLAGEETQKIIKTIDEIAFQTNLLALNAAVEAARAGEAGAGF
                     AVVADEVRNLAMRAADAARNTAFLIEGTVRKVREGSLLVEKTNREFREVTSTVIKSGE
                     LVREIAAASREQAQGIQQINQALSEMDKIAQQNSANAEESASASEQMSGQAGQMKSFV
                     GQLARIIGRAVTRSIQESESRRTPVSRVEVKIREEAKPLLASSRHPGNGSAKGNGSAP
                     RLETGRPTHPGRLIPLDEDDLKDF"
     gene            complement(22584..22958)
                     /locus_tag="PLM79_02900"
     CDS             complement(22584..22958)
                     /locus_tag="PLM79_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85280.1"
                     /translation="MRLLLYFSDSETAGRKLLEAVYKRIPPEETEVHRDMKSLSDRFR
                     RPRGELRVAVILCSSREELSQIVSLGPLISDLRILLLLPDRERETIARGHTLYPRFLS
                     FVDESFEEVGAVLGKMLARSGS"
     gene            complement(23431..24849)
                     /locus_tag="PLM79_02905"
     CDS             complement(23431..24849)
                     /locus_tag="PLM79_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85281.1"
                     /translation="MARVLIVDDDPDTSELLRELVRRVGHEAACAHTLARGMEELLAA
                     PYDVVFLDVRLPDGNGLDLLPRIRETASSPEVIIITGYGDADGAELAVKSGAWDYIQK
                     PFLAQTILLPLKRVFQYRENLRECRKQTVTLKFDGLVGNSAPMRACLDQVAQAANSDA
                     HVLITGETGTGKELFSRAIHDNSSRRNRSLVVVDCAALPETLVESFLFGHEKGAFTGA
                     DRAKEGLVKQANEGTLFLDEVGELPLSVQKTFLRVLQERRFRAVGGKEETRSDFRLIA
                     ATNRNLDRMVEEGLFRKDLLFRIRSISIELPPLRNRPEDIQELAFHYLSKLCKNYDIE
                     GKGFSPDFLEAITQYHWPGNVRELINALESAISAARYEPILFLKHLPTQIRVQVMRSA
                     MAKGEASGGRESRESSMDSKDFPSYRDLRNSVLEEAERKYFENLLEYTHGNLKEACRV
                     SRLGRTQLYMLLKKHGLSRSDR"
     gene            25622..27010
                     /locus_tag="PLM79_02910"
     CDS             25622..27010
                     /locus_tag="PLM79_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOV85282.1"
                     /translation="MDVVLLSRWQFALTTAFHILFPTLTIGLALYLVVVELLWLWKKE
                     EIYYRLYRFWVKIFAIHFAVGVVSGITLEFEFGTNFARFSQAVANVIAPLMAYEGMTA
                     FFLEAGFLGIMLFGWNRVPRGIHFLSTCLVALGAGLSAFWIMAANAWMQTPAGYRFAE
                     GRFYVTDFLSAIFNPSLPTHLSHMLLASYETAAFAVAGISAYYLLRGRNPAFFRKSLS
                     LALVMAAVAAPLQVLVGDFKGVVVAKHQPPKLAAMEAHWNTNTRGGAPFVLFALPDMQ
                     EERNRWEVTIPHVLSLLITRTWDGKVTGLKEFPPGDRPNALITFWSFRLMVAVGFLYL
                     ALMGWAAFLWRRKRLYDSRPFLVALVAVQPLGFAATVSGWVTAEVGRQPWLVYGILRT
                     ADGVSPIAAGNVVWSLLLFLGFFSLIGTSYFYYVLKTLHRGPDFSSPIPPIQRPAGFR
                     PIQGVAQGTEES"
     gene            27010..28005
                     /gene="cydB"
                     /locus_tag="PLM79_02915"
     CDS             27010..28005
                     /gene="cydB"
                     /locus_tag="PLM79_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOV85283.1"
                     /translation="MDLVNLWLCLVGLVILLYVVLDGFSLGVGILFPTARNEAERDVL
                     MNSIAPVWDANQTWIVFGGGALFATFPMVYSVLFSALYIPLLTFLFGLIFRGTAFEFR
                     ANSTRKSPWNRAFFGGSLTAVFALGVTLGSYLSGTRVVNGQFAGGAFDWLNPFSVMVG
                     LALIGGYGLLGATYLVLKTTGPVQERAYRQAFFLAWVVAGFMLLVSVWTPLHDPSIPA
                     RWFSVPRVYFIWSFPLLGVGAFLTLIRSLRKRRELLPFWSSVVLFVSAYLGLLAAVYP
                     YAVLPGVTVYEAAAQRETMLFTLWGVCLVLPVVLAYTVYSYWVFRGKVVAEEGYH"
     gene            complement(29476..30603)
                     /locus_tag="PLM79_02920"
     CDS             complement(29476..30603)
                     /locus_tag="PLM79_02920"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOV85284.1"
                     /translation="MNNREYRLVDLFSGAGGLTLGFTKSFGHSFLPIWANDSNESAVQ
                     TYNKNFGKHCIHGDILSLLSRADGRIPFADVVIGGPPCQGFSLLNKQRTQDPRKQLWR
                     PFLEVVQKTGSRVFLMENVPQLLGSPEHEEIVREAEALGFVCTWAKLCAADYGVPQTR
                     WRAFILGSRIGDPTRFFPPRKTHRNPSNGTSSESEYVPNPLPWRTVRDAIADLPPPVG
                     VHIREDSPPLDLHFGRNPTAISLRRYKTVSKEGMNRIDLQRLAPELTPDCWIRKESGG
                     TDLFGRLWWDRPAFTIRTEFFKPEKGRYLHPSQHRPITHREAARLQTFPDDFRFCGSK
                     IEIAKQIGNAVPPLLAARLADCVTELIRSSEERDDVRQAHA"
     gene            complement(31248..32477)
                     /locus_tag="PLM79_02925"
     CDS             complement(31248..32477)
                     /locus_tag="PLM79_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85285.1"
                     /translation="MINPSPIDDEPGKPGTDPLSFRRHLGPILFLTAIFFVNFIARII
                     PSPLMPTIEKDLNLDHGQAGSLFLLIASGYFLSLTTSGFVSHRLRHRKTILLSCAALG
                     ITFLGTSLSGSLSSLRAGLFLMGMAAGLYLPSGIASITSLVAPKHWGKAISIHELAPN
                     LAFVCAPLFSELVLSRASWRAMVGLVGGASLLLGLAFSRFSRAGDFPGQKPSFGALRP
                     LLGQSSLWIMILLFSIGISGSQGIYSMLPLYLVSEQGMGESWANSLLALSRIPTPALA
                     LAGGWASDRFGLRRTMTVSLLLTGLSTLLLGLAPSSWIVWVIFVQAGLSVCFFPAALA
                     AMSLIGPPQSRNVVISVIISMAFLVGAGGAPTLIGILGKTDSFAHGISVVGTLLVLAG
                     VLASFIRLPRRGETGPS"
     gene            32576..33295
                     /gene="sfsA"
                     /locus_tag="PLM79_02930"
     CDS             32576..33295
                     /gene="sfsA"
                     /locus_tag="PLM79_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HOV85286.1"
                     /translation="MQRDPKDPLLVFPRGSFSARFVRREKRFRVEVETQEGRTWVHCN
                     NSGSMLGLLRPGSPVLVSPAPGRGRILAHTLELIRVGGIWVGVNTLVPNRVLRVAWEM
                     GLLPELEGCDSFQNEARSGDSRLDARIRGAAGTVWVEAKNVTLVENETAYFPDAATTR
                     GQKHLRELAALTRCGDRCACFYLIQRQDARCFSPADFIDPAFAEIFWKALDAGVEVWP
                     YQARVSPEGIGLGPRLPLSKR"
     gene            33465..34835
                     /locus_tag="PLM79_02935"
     CDS             33465..34835
                     /locus_tag="PLM79_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV85287.1"
                     /translation="MKPSNTKSWAVFRAAILCLMLAVPAVGWCRESSLPREEALTLDQ
                     ALAMAMEKNRLVRSARLEADKAENKVAEARTNLLPILSFDLFEMYALTSQTYTFERGL
                     LGTIPNLGPIPRKSTRVDISPDLLSIVSLTATQPISQLYKIALGIRQAVVLRDMAREE
                     LRARRQSATYEVKKAYYGILQSQSALGAAEDTIKLDRELDRLTDDYLAQGKVLKADSL
                     GVKSRLARSEHEAFSLRNALATRKEQLNDLLGRDVKTRFRVSPVGDLTLFEADLDAAE
                     ARAVEQRPEVREARLKVKQAEYEKKIKKAEYIPDLSLMANYFSPVGSRFIPDNVAVVG
                     LYLRWEFFDWGRKQKELVQRADAVEQAHLALREAESKVVVDLNAQYRTVREARSLLGV
                     CELAREAAREQLRVSLERYSRDAALVKDVLQAQAALSEANHRYEESLLAYWTGRADFE
                     RALGEE"
     gene            34836..35960
                     /locus_tag="PLM79_02940"
     CDS             34836..35960
                     /locus_tag="PLM79_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV85288.1"
                     /translation="MKTRACWIWISLALAWLGGGCAREEPYKKPLTPVRVEEARIATG
                     DGGVRYSADVQPYTQVNLAFKVAGYVESILQKRGADDRMRNLQEGDFVSAGTILSTVR
                     KEDYLQKVKEAKAQLSAARALLEKARRDFDRAVHLLATRSLTRVDYDAAQAQFEEARS
                     RVSGAGAQVDEALIHLGHCELKAPMDGVVLKREVEVGSLVNPGTVAFVLADLSSVKVV
                     FGVPDQMLPYLKLGDSLAITTESMPDKEFRGLTTALSPLADPKSRIFNVEVTVPNPDN
                     LLKAGMIAALQVARPDRPRQVPVVSLKAVVRSRTDPGGYAVFLVEGREGVATARLRNV
                     VLGDVVGNKIEVLEGIQPGDLVIVTGSTLVADGESVRVIP"
     gene            35979..39623
                     /locus_tag="PLM79_02945"
     CDS             35979..39623
                     /locus_tag="PLM79_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV85289.1"
                     /translation="MTHKSDAEIIRETRNTARYFAENRHIAWVLLVVVILWGIYGYVN
                     MPKRKDPDIPVRLAVALCPWPGVHAEKIEQLVTRKMEAKIAENSRIHPPSDYDFGIMS
                     ISLNGLSIVYVQLGEDVEDTKKEFNDIHLKLDGLKDLPEGAGPIRFISDFGDTAALML
                     TVASPKEGPDEIALRAQSIRRAIRGVRSGAAADGRGSRATLVVALPFATNPQVLSRGL
                     HLFGQYMKENSLGWDLRPIQGSGFLALDFATCAEDEAVFDLVGRFLRERIHEEEVHPD
                     AWMPVIVRDPEDTEAKLAAVAAEKYSYRELDDFTDLIQRTLQPVPQVSKVDRTGVLGQ
                     EILLEYSQERLASYGIQPYKIGDILRARNINLPGGVLQIEGKEIGIDPSGEFKNEKEI
                     GNVLIPASPSSMPLYLRDLVDIHRGYESPPRFLNYYSWRDARGEWHRSRAVTLAVLMR
                     SGEQIGAFGAQIDKALAEVRQQLPEDLMIVRTSDQPRQVSEEVSLFMKALYEALILVV
                     LVAWLGFWEWRSALLMALSIPLTLAMTFGMMDLLGVDVQQVSIASLIIALGLLVDDPV
                     VAGDAVKRDLALGHPSLTASWLGPTKLAKAILYATITNIVAYLPFRLLSGDTGLFLAT
                     LPIVMACSLISSRLVSMTFIPLLGYYLLRPPRKPEPSLEERRTRGFTGFYYRMGSAAI
                     RHRWGVFTGSLVFLGVGALFFSQLKTSFFPDDLQYLSYVDVWLPNDASLLTTNEAAVQ
                     AERVVEEVADEYGRTHPGKDGKPRQVLKSVTTFVGGGGPRFWISVSPELQQQNYAQLI
                     LEMENKEDTPLLARPLQQALSARIPGARVDVHQLQINPVDFPVEVRISGRADLGLRKD
                     DADVRVLRRLSHEVKEIFRSIPQADRVRDDWDEESFVVSLQVDPDRANLAGISNLDVA
                     GSCASAMNGIPLTTLREGDKEIPVLARLRMEERARLSDIKSLYIYSIENANKVPLLQV
                     STVHSQMRTERIRRRDHFRTFAVHCFPVPGALASEVLEQAYPRLKALEDRLPPGYRMD
                     IGGEQAKQDQGFAELAMVLAISAAGIFLALVFQFNNAVKPFLVFAAVPYGMVGALGAL
                     YVMGAPFGFMAFLGIASLVGVIVSHVIVLFDFIEEMHEKGEPLRESLLDAGIVRLRPV
                     MITVGATVLGLFPLAVHGGPLWEPLCYAQIGGLSVATFIELILVPVLYAIFVEDLKLV
                     RWKGTGEPSS"
     gene            39719..39925
                     /locus_tag="PLM79_02950"
     CDS             39719..39925
                     /locus_tag="PLM79_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85290.1"
                     /translation="MEDRTLRIGRFFTDTRILRCFASNCRFHLKDCCNLKQVSVDPEA
                     RCTGYEPRKAALPGESKLEMENSD"
     gene            40511..40960
                     /locus_tag="PLM79_02955"
     CDS             40511..40960
                     /locus_tag="PLM79_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85291.1"
                     /translation="MRRIEGVRGTVVCGLVIFLAGCGMFQRRSDGPSFQVLDVNGDGQ
                     VNREEYVNGVLMDSFRKVDADRDQKITREEWAKVNKDPDAAAQFDRLDTNRDGVVTLQ
                     EWIREVEKHTNVTETFDEKDARRDNFLTVDEYSRGRNSGFTIFNMKF"
     gene            complement(41003..41317)
                     /locus_tag="PLM79_02960"
     CDS             complement(41003..41317)
                     /locus_tag="PLM79_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV85292.1"
                     /translation="MPVYEYEHCTEPCEWGKAFEIRQEIREEPLSRCPNCGRDVRKIL
                     SRVNINTPKTNTELRDLGFTKLVRRDDGVYENVTARPGESRTMTRDNPQSVPRLDRIL
                     KD"
     gene            complement(41419..42048)
                     /locus_tag="PLM79_02965"
     CDS             complement(41419..42048)
                     /locus_tag="PLM79_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOV85293.1"
                     /translation="MDKTSERPNGSTGPSAASVEHLSQHQLRRLSEAFELWHRRASSS
                     YIRRVRGRYWLSYLFLRFTGARIGEVLRLNDLADIDFDRNEVRILRSEYRNLRNVLRT
                     VPVPPLVTRQVLDYRDEFPGMRGRVFALDQGNFRREFYRRAQEADLPRDLSHPHILRH
                     TRAVEMLRAGVPLTTVQELMGHVLSGTTALYLQRTEITVRKVLEDKGLL"
     gene            42289..42744
                     /locus_tag="PLM79_02970"
     CDS             42289..42744
                     /locus_tag="PLM79_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOV85294.1"
                     /translation="MAIIRYPSLWSGMNPFRELERMRKEMDRLFSEVMDRSTHLQSSG
                     VYPSLNVSQDGEQFFVQAELPGIHPEDLEISVEGTTLTLRGERKSENVEGVSYHRRER
                     PSGRFHKALTLPHEVDSDKVEATFKDGVLKLVLPKAEHVKPKKITVRAD"
     gene            42803..43201
                     /locus_tag="PLM79_02975"
     CDS             42803..43201
                     /locus_tag="PLM79_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOV85295.1"
                     /translation="MEDKDLQVQEKQELKTKSESTRNVPIYIPPVDIFESPSELVLMA
                     DMPGVAFDNVDIDLDNDQLTIRGSVRVEDEKGTVLLKEYSVGDYYRQFTLSNVIDRNK
                     IEASMKDGVLKVVLPKAEAAKPRKITVKST"
     gene            43383..44237
                     /locus_tag="PLM79_02980"
     CDS             43383..44237
                     /locus_tag="PLM79_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOV85296.1"
                     /translation="MKLRKTVSLTAFLSFMLVILTSVILYIVPQGRVAYWADWRLWGL
                     SKTQWTEMHLNLGLLFLIALGLHVYYNWSAIVSYLKDKTRTLVLFPREFSAALVLVLV
                     FGVGTYLEVPPFLWVRELSTHFKEEAARKYGEPPYGHAELSSLESYTGRMGLDLDQSL
                     AGLKKAGMAYDHPGQTLLEIARRNGVPPKVVDQALRSAHPPVHGSEVRKLPDSPPAGF
                     GRRKIEEIAGEYGLDVSHLVRDLTRAGIRATSQESIKEIASRNGKAPVEIYELIRTHQ
                     NKGAPVSP"
     gene            complement(44382..45542)
                     /locus_tag="PLM79_02985"
     CDS             complement(44382..45542)
                     /locus_tag="PLM79_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85297.1"
                     /translation="MLPIILVGSAVTLFLVVLLVFLFVASRKRRRVLDLLAQKVGAVG
                     SGRKFTAVHEGVPYRYEYFPGSGNSPSRFQVATDSPLEGSFKIGREKAFDRFSKNLGI
                     AREIQTGDPDFDREFYISSSDEEFARGFFSDAQRRQAVRDVFRLGFTEIKLDKKGMEA
                     VLTPFPLREDIDPSFLPAAAQLLIRLAQDPSVSYVPPAYIEDPSWKTRRIAAFAIPSL
                     LLVAGTASWIWGGAEFTPLDPEDVFLDSLKYSLPGLGIFLWWAVRALVGRSTSHKELL
                     VVLVLSLAGFLVAGGGAETLANGILDTSPHSGHEARVLQKYVTRSKNSTTYHVQVESW
                     RSPTQPETISIRSSDYGRIQPGRTTVLAVTKPGRLGFEWLVAYEIRNPLIRK"
     gene            45731..46672
                     /locus_tag="PLM79_02990"
     CDS             45731..46672
                     /locus_tag="PLM79_02990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOV85298.1"
                     /translation="MERIRNYRELLDALFDGVYFLDPERKIVYWNSAAERITGFSAAE
                     VVGKSCADGILRHVDENGTDLCKGLCPAGKTLRDGLMREADVFLHHKEGHRVPVSVRV
                     TPFRNRKGEIAGAIELFSDGSSKNAMIEKIRELEGLALLDPLTLSGNRRFLEMSLQGR
                     LQEKARTGICFGVLFADVDHFKRVNDTYGHQVGDQVLRMVCSALRSSLRPFDDLGRWG
                     GEEFVVLLRNVDQRGLERVAERLRIMVKKSFLSLDGLEIGVTVSMGGTLARDEDTPTS
                     LLQRADEHMYRSKIAGRDCTHTDEGRIPPLSDPKGPA"
     gene            46733..47062
                     /locus_tag="PLM79_02995"
     CDS             46733..47062
                     /locus_tag="PLM79_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85299.1"
                     /translation="MNDPEKKTWRERLSEGSARGRKKFQSLRRAVGEIFRGMTVHELE
                     MELRKEKATFDQLFLLIVFGDLIGLPLFPPYYTMRLLPYILPGIESWKRSVLREKDLS
                     DLVSLDM"
     gene            complement(47089..47745)
                     /gene="thiE"
                     /locus_tag="PLM79_03000"
     CDS             complement(47089..47745)
                     /gene="thiE"
                     /locus_tag="PLM79_03000"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOV85300.1"
                     /translation="MNRDERKALFQEVDVYPVTCQRLSAGRTNREVLEEVLRGGAKIV
                     QLREKELSTLEFYRMALEFRERTAAAGVLLLINDRLDIALAVEADGVHLGLEDLPLAV
                     ARRLAPEMLLGASTHSIEEALEAQENGADYINVGPIFPTGTKEGIKKFLGPRAVTEIG
                     ARVRVPFTVMGGINESNLDQVLSAGARRVAMVTAITQAPDIAERVRSLRRRIAAHATG
                     "
     gene            complement(47746..47946)
                     /gene="thiS"
                     /locus_tag="PLM79_03005"
     CDS             complement(47746..47946)
                     /gene="thiS"
                     /locus_tag="PLM79_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOV85301.1"
                     /translation="MEITVNGKPRHLESPTTIIGLLELLGINPKTVVVERNLNIVPRD
                     AMESEPVAEGDSIEIVRLVGGG"
     gene            complement(47988..49100)
                     /gene="thiH"
                     /locus_tag="PLM79_03010"
     CDS             complement(47988..49100)
                     /gene="thiH"
                     /locus_tag="PLM79_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HOV85302.1"
                     /translation="MSFHDQMSQHPWKEIAAQVRGRSASDVEAALAGRSVNLEGMLSL
                     LSPAATPYLEEMARRAHRLTLQRFGRTIQMFAPLYLSNECVNACVYCGFNRHNPIERR
                     TLSVDEAEAEGRAIHDLGFRHLLLVSGENPHRVTLDYLRQVLDRLRPRFSSISIELHP
                     MGVDAYEALIDAGVDGLVVFQETYNRERYGDFHPQGKKRDFLWRLETPERGARAGFRR
                     VGLGALLGLCDWRVEGFYLALHAQYLFRNFWRSQVNISFPRLRPAAGRFEPPHPVSDL
                     HFVQLLLALRLFLPDAGLVLSTREPPSFRDHLIPLGVTTMSAGSHTEPGGYSRPTDAE
                     AQFSVCDDRPPRAVAEVIRNRGYEPVWKDWDAAFLQ"
     gene            complement(49106..49888)
                     /locus_tag="PLM79_03015"
     CDS             complement(49106..49888)
                     /locus_tag="PLM79_03015"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOV85303.1"
                     /translation="MNDPLIIAGRVFHSRLLMGTGKFSSPQIMKEALEASGAEIVTVA
                     LRRVDLEDPGDSILNAIDTGRTLLLPNTSGARDADEAVRLARLARAAGCEPWVKLEVT
                     PDPQYLLPDPVETLKAAHILVKEGFVVLPYIPADPILAKRLEEVGTATVMPLGSPIGS
                     NRGLKTRDSIEIIIEKARIPVVVDAGLGAPSHAAEAMELGADAVLVNTAIAVAGDPVA
                     MARAFRKGTEAGRQAFLAGPAKPRKTAEASSPLTGFLRLEQG"
     gene            complement(49905..50675)
                     /locus_tag="PLM79_03020"
     CDS             complement(49905..50675)
                     /locus_tag="PLM79_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOV85304.1"
                     /translation="MPGEPTRLTTRHRERFRCHVLLDRFGEQGQIKLLGSRVLVVGAG
                     GLGSSALLYLGACGVGEIGIADGDRVDLSNLQRQVVHGEEDLGKEKTRSAGETLRRVR
                     PEVRLNLHSSRLTPDNAEEILAPYDFVLEATDNFESKFLINDACMRLNKPFSHAGAVG
                     WYGQTMTVVPGKGPCFRCLFQEPPPEGAVETTSKAGVLGTVPGVLGLIQATEAVKYLL
                     GAGDLLVGRLLTWDALAGIFREVRLPQEKRCRVCGNYS"
     regulatory      complement(50730..50824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         9555 a        15402 c        16153 g         9714 t
ORIGIN      
        1 gtcgctggtg ggcaaaccgt tgatccacag cggcatccgg gaggaaaccc agtgcagcgt
       61 gatcgccgtg aagaccggtg aggccatgga aatcaacccg gacccccggc agcccattcc
      121 ccccgacgcc cacctgattc tcatcgggac caccgagggg gaaagacgtt ttgccgacag
      181 gtatccggaa gatcacgggt agtgagtctc ccggtccggg aagccctctg ccgcgaacct
      241 gcctcagttt tcgaaacggc cgtcggaaac ccggaaccgc ttgatcagct cgccgctcaa
      301 ggcttcccgc acctccacct gggattgtcg ggtgttcgaa ttggtcccca ggaccgcaag
      361 gtgccgctcc cctcgcacca tgagttcctt gggttcgaag cgagggtcaa agtcgatctt
      421 actgatcaac cgtccggtag ccgagtccct gaccaccacc cgcaccacac cggttttttc
      481 atgaagagcc aggaccgcca ggtcctggga cccgttggcg ttgaggtcct cgagggcgac
      541 cattcccacg ggggtccacc cggacccgaa gaaacggatt gtcgtggcgg ctgcaggggg
      601 gggcgcatcg tcgtggacga tgtggacctc gatttcacgc ttgtccaggt catggcgcag
      661 cagggccatc tcttcgatcc cgttgccgct cctatcctga atggagaccg cgtcccgggt
      721 cagaaaggag cccaggttga tcttgggagc cccggtcttg tcccagattc cccaatgggg
      781 ggcccactgg ttttccgact tccagggctc gtcgaaggcc tcgaagagga ataccggccc
      841 cagctgggga gctctcgaga ggatttgagt gatgcaggtc tgctcgttgg gaatgctggg
      901 caccgcggca ccgttcttgg ggccgatgct gggccagccg gtctccccga ccaccatctg
      961 cttgccgggg aattgcccgg aaaagtctcg gtaggcaccg gccaggttgt tccatgcccc
     1021 gctgatgtcc actccgccgt aaaagggata gatgttcacc atcatgatgt cgcagtaggg
     1081 catcagttgc tttccgtatt tccgggcagc cccgtaggcc aggcaggtgg tcaccggaat
     1141 attcttgttg gcaatggctt tccgtacgta ctggagatcg gcgatgagct gattgacgct
     1201 cacgggaaca tccgtggaat ctcccgggag acactcgttt cccaccacca cgcactggac
     1261 ggtattgggg taggtgttgg cctggtggat ggccatgtcc agctggggcc gagtgaccgc
     1321 agcatggtgg ggaccgtcct cgaagactcc caggaagact ttgagctgcg ggaaattcga
     1381 gccggccagg ggaaccaggc ggtcgagccg ggaggcggaa tccagtccgt aggttttgat
     1441 atacttaaat ttcttggtga taatccccag gtcggtcaag aactggctgt ccggaatggg
     1501 ggtccccgag cgctgtccat ctttgtggaa gggcccgtaa ttgacggcca cgaaattggg
     1561 caggccggcg gcagcaaccc ggcccaccgg cagtccccag gtgaataccg atacgagaac
     1621 ggcgatggaa acgcacaact cggcaacctt catgacggag gctcctccct tttaggtggg
     1681 ggtggcaggg tttctcggct gcttggtgca aacccatact cctcaaaaag tccggatttg
     1741 tatagagctc ttttggtccg gtcgattctt tgcgggcgag ggcgggccaa aaggtccggc
     1801 ctcgacggcc gggagacctc aagaggggga ctgaccggac aattccttga catcttttgg
     1861 gagaaagact gttatatcac ttatcccaac agtccggttc ggtcttgggc gtgggccgcc
     1921 aatggaaaaa ctcgagtccg gacctcgtac tttttgccga cgtttgtttg atgtttcgcg
     1981 gggatgcttc gggtagcgcc cgtccccctc gtcagaggag cctctatgga aaggaacgac
     2041 gaagccaaag gagcagaaga gggctccccc cgggagaatg cctcaatgac ccggcgggct
     2101 cttctcaaaa ccggcctcgg cctgggggcc ctggcttcca tggggggggt tgccccggat
     2161 tgtctcccgg gaaatcccgg tgcggcgggc aacgccggaa ggctgggtcc ggcaggggcc
     2221 tcccattggt tcgaaggttt cctattcaag gacgaaagcc tggtttttga actcatccgg
     2281 gtcctttcca agatgactag tcgcggggcc gacctggggg agtctctctc cgcggcgatg
     2341 cgcatccggg aaaaggaagg cgatacccgg gcccttttgc aggcctggta cgaagaatgg
     2401 cggaagctgg cggagcgggt cgaaaagatc ggagaccgct gccttgccgg agggcatcga
     2461 gtgagcgcgc gggacgctta tttccgggct tccgagtact accgcagcgc ggacttttac
     2521 cttcacgacg acccccgcaa tccggcgatt ctcgagttgt ggaatcagat ggagtcgtgc
     2581 ttcgagaagg cccggaagct ttcccgtcct tccttcgaga cggttggcat tccctacgag
     2641 gggacccggc ttcccgccta tttccgcccc gccggagtca agggcaggcg tgcccccacc
     2701 ctcatcgttc accaggggtt cgacggcacg atccaggaaa ccttcctggc ttacgggaac
     2761 gatgccctgg agcgggggta caactgcctg gttttcgagg gcccgggaca gggcagcgtc
     2821 atccgaaaac agggcctccc cttccgacac gactgggaga aggtggtgac gcccgtggtc
     2881 gactataccc tgaagagaaa ggaagtggac ccgaaacgga ttgccctcat ggggttgagc
     2941 atgggcgggt acctggcgcc gcgggctgcg gccttcgaac cccggcttgc cgcctgcatc
     3001 gccaacccgg gagtcctgga tttctgggcc aagacctacg aaaagtcggg gttgaagcga
     3061 aacgaattca aagaatttct cgaaagcgct cccgaggaat acgacaagat atccagggac
     3121 ctggcgagca ggagcatcac cttctattgg gccgtgaccc acggcatgtg ggtgtttggg
     3181 gcgaaaaccc cggcggaact ggccctcaag caagcggcct ggaccctcga ggattgtgct
     3241 tcctcgatct cttgtcccac cctggtgatc gattccgagg gcgagcagtt cgacccgggg
     3301 caggccaaga agctctacga ggccttgagg tgtcccaaaa cctacctcct cttcaaggcc
     3361 ggggaaggag cggagctcca ctgccaagcg gggggggccc tcctgggcac ccagcagatc
     3421 ttcgattggc tggatgaaac cttcaaaagg gtgaaacact gacgcccaaa ggaagaaatc
     3481 tcagccccga ggcggcagga agcaggattg attgccgtaa taccaagttg cgcccaaggt
     3541 cgccctgaca tgctgagtga agatttcgag caaagggatg aaccctacgg gtgggcagtt
     3601 tcggcttgaa tgcaacttcg tatcaggggc aggccgtccc cggtgccggg gactcgatag
     3661 gtcggaaagg acgtccatgg gcggatttga ggatcgagtg ggggcggtga ttccggctgc
     3721 cgggtattcg tcccggatgg gagccttgaa acctttgctg ccgtttgggt cttccacggc
     3781 cgtcgagacg gcggtcgatc tgtttctccg gtctggaatc cgggacgtgg tcgtggtgct
     3841 gggataccgg gccggggaga tcgttcccgt tctcgatgcc cgtcgaatcc gttgggtact
     3901 gaatcaccgg tacggagcgg ggatgttctc ctcggtcctg gccggaatgc gctgcctggg
     3961 ccccgacatc tgcgccttct ttgtgctccc ggtggacctc ccgggtgtgc ggtccgaaac
     4021 cgtcgggatc ctcctcgatg tctaccaaca aaacccgcat tccgtgatcc gtccgcgctt
     4081 tgggaatcgc agggggcacc cgcccctcgt ccccgtccgg tgcgttcccc gggacattcc
     4141 cgccgacctt ccgggggggc tgaggaccct gctgcgacgg taccgcgacg ccaccatcga
     4201 tgtggaggtc cccgatgagg gcgtcatttg gaactgcaac accccggagg aataccggag
     4261 gatccgggcc tcggccgctc ggcaagacgc aggagaaggt ttcccgagac ccttcgacga
     4321 tagtgctcat ccgcccgcag atcgctgatg ggggagacgg cttcccgcac aagggctgcg
     4381 gcccgctgca acgtgtccgc agagagcctt tgacccaaga gcgcctcttc ggcctcgggg
     4441 catcggacca cggtggggcc cacgcttccc caggcaaggg ccgcccggcg caccaccccg
     4501 tcgggggaga acccgatgag cgctgccagg ctcaccacgg cgcaggccag ggcgttgcgg
     4561 cgcccgacct tctcgaaatg atgaagaagg tcccgcgccg gtttcttcac ccggattccc
     4621 gtcaggattt ctccttcctc gatgcaggtg acgccgggcc ccctgatgaa ttcccgcact
     4681 ggcatccgcc gaacgcggcc cccggaccgc agctcgacct cggcttccag cacgtagagg
     4741 ggaggaaggg tgtctcccgc gggggatgcc gtacagagat tgcccccgat ggtccccatg
     4801 ttccggatga ggggcgagcc cagggtcttc acggcctggg ccagcacggg caggtgccgt
     4861 tgcaccaggg gatgtgccag gagacgggaa tgggtggtgc acgagccgat atggatgtgg
     4921 tccccgtgtt cgttcactcc tttgagctcc tcgattcgtt ccaggcacac gagggcggcg
     4981 gggttttcca tgccccttcc cattcgcacc agcaggtccg tgccccctgc atacagggcg
     5041 gcccgggggc ggcgatccag gagcggccag agctcctcga gggaccgggg aagatgtacc
     5101 tccctcacga cgaaccctcc ccccgcatct tggcggctgc cgcctcgacg gcgtccacga
     5161 tcatggtata gccggtacac cggcacaagt tgccgctcag cccctcccgg atctcttccc
     5221 tcgtgggacc ggaaagattc tcgatcaggt gcaccgccga aagcaccatg ccgggggtgc
     5281 agaatccgca ctgaacggcc ccgtgttcca caaaggcctc ctggacggga tgcaggtctt
     5341 ccccctgcgc caggccctcg atggtggtga cgtggcgccc ctcgatctgg gcggccaaca
     5401 tgagacagga taggcggctt tccccgtcca ccaggatggt gcacgcgccg cattcccccg
     5461 ccccgcaccc ttccttgacc ccggtgagac ccaggtcctc ccgcaggagg tccgtcactt
     5521 tgcggtcggg ggaagtctcc acgagggtgt ttctcccatt cagtacaaac cggatttcca
     5581 cctcattcct ccttgtgcgt tcgggaacgg cgggccgccg cagcggtccc ggcggcgcct
     5641 tcccggttct cgacggctcg cagcacccgg ccggggtcca ggggagccac ccccggacag
     5701 gatcccaacg catcctcgac ggcacccgcc acggcgggaa ggggcgcatt ggtcccgact
     5761 tcgccgatgc ctttcattcc gaaaggaccg gtggactcga cggtctcgac ggccagggac
     5821 cggatgtccg gaacgtccag ggacgtggga atgaggtagt cggtgaagct gggattgagg
     5881 attcttcctt tctcgaggag cacttcctcc atgagcccat acccgattcc ctgggccacg
     5941 gcgccctgga cctgctggtc gaagccctgg gggttgatgc ggcgcccgcc gtcggtcacc
     6001 gcgaggtact ctttcacctc gattcgtccc gttagttcat ccacctcgac gtacgccagg
     6061 tgggcggcgt aggagaagag aacgtggggg aacccgatgg cgaactcctt gcccgttacc
     6121 caggaccggg catgatgaac tggctcacga cgacacggtc ggattcggga aaaacgcggg
     6181 ccagggcgtc caggggcacc tctcttcccg aggggaggtg acgcactttg ccgggcagaa
     6241 gggcgagtcc ccggggctca tccaggagaa gcatcagggc ggcccggtgc aggagtttgg
     6301 ccttcagttc acggcaggcc tcgatgaggg cgtttccata ggtataggtg gtgcggcccg
     6361 ccgaggaaga acccgaaggg tgggtgcggt ccgtgtcggg ctgcaccagt tccatatggg
     6421 aggcctcctg gcacaggatc tccccggcaa tctggacaaa cgcgctggcg tttccctgtc
     6481 ccatgtccga gaccccgctg taaacgcgga ttctcccttc ccgggtcagc tcgacccggg
     6541 cgatggcgga atcgggcaat ccgcgaccgt accccatggc gttccaggcg gcggcaatgc
     6601 cgacgccccg gcgtttgaag ggccccgggg aatctttcca cttcctcctc cgggtccaca
     6661 ggggatgtcg gcccagggtt tcgaggcatt gggcgatgcc cgtcgaatgg acgagggtca
     6721 cccccgaggt gttggtgtcg ccgcggcgca gcgcgttctt caggcggatt tcgaggcggt
     6781 ccatgcccag tttttccgcc aggcggtcca tcatcctttc gatggcaaag gtaacctggc
     6841 agaccccgaa gccgcgcatg gccccgctta cgggatggtt ggtgtacacg cagcggcctt
     6901 cgatgagggt attgggaatg cggtagggtc cgccggcatg ttccatgccc agggccatca
     6961 cttctccgcc caggtgcgcg taggctcccg tgtcgtagac gagacggcag tgaagggcat
     7021 ggagggtccc gtcggccagg gcccccagtc ggtagtgcat ctgaacggga tggcgcttgt
     7081 agcccgcggc gaagctctct tcccgctccc accacatctt gacgggcctt ccccccgcat
     7141 gcatcgcggc caggaccagc aggcactgaa cggtggtgcc gtcctttccc ccgaagccgc
     7201 ctcccaggta cggggcgatg atccgcacgg catccgcctc gatccctagg gcatgggcga
     7261 tctcgaaccg gtctcgaaac ggagcctggg tcgacacgat caggacgatt tttccgtcgg
     7321 gctgctgtcg ggcaatcccg ttttcggttt cgagaaaagc atgctcctga gcggggactc
     7381 ggaaatcgcc ttccacgacc acgtggcatt cgtccaggga tgtcgtcgcg tttccgaccc
     7441 ggatgacggc ctcgaggaga aggtttccct tctcatgaac ccggggcgcc tcgaggtgta
     7501 aggcttcctc ggggtcgaag acgccgggca ggggttcgaa ctccacgcgg atgagatcca
     7561 gggccgctcg gagggtttcc cggtcctcgg tcagcaccat cgccacgggc tcggcccagt
     7621 ggcggacctt gtcttcggcc agcaccggct ggtccttgtg aacgatcccc tgccggttgg
     7681 atccccggat gtcccgggcc gtcaggaccg catgcacgcc cggcagcccc cgggcctccg
     7741 ccgagtcgat tccccggatt cttccgtgag cgatccgggc cctcttgacc ccggcccaca
     7801 gaaggttgtc ttcatagtga tccagggcgt acttctcgcg tcccgtgacc ttggccgcgg
     7861 cgtcatggcg cggggaacgc tctcctactc cgaacacccg gtctttcacg aattcctccc
     7921 tgttctcacg tgggcggatc ggccccggcg gccgcctttc ccgaagtctt cgtcccgtaa
     7981 ggtttcagtg ggatcagcag aagaaacgcc atcgagagga cggccgcgca gagggcgaac
     8041 aagaggtcga aggagccccc cgcggccagg aacgctccgc cggcatatgg gctgagaaag
     8101 aagccggcgt ccatggcaaa gagggccagg ttggtgttca atccccgcaa cgtgggcggg
     8161 gaggccagga agagggccgc gttgaggagg ggcatgagga agccgatgca gagcccgtag
     8221 caggccgcca tgatcccgag ggtgcccggg gaatccatgc ggccgaaaag aagatagcag
     8281 gggatgagca agaggatgaa gatccgcagg gcacgtgtct tgtccatcct gtccatgaaa
     8341 cgcccggcca ggagtcgaac cgcgatcatg agcagggccg agacggtgaa gaagctcgcc
     8401 acatttggga tccccttcgt ttccgcatgt cccttcataa agaagaagac ggtggcgtag
     8461 aaaaatacac gaggaggttc actgccagca ggaggactac cggtctttct ttgaggtcca
     8521 ggcggatttc ctcgggggtg ggtcgccggg tgagcgcgcc ttccaggctg cacaggacct
     8581 tccccaaccg tcctcgaagg acccggagca cggcgaaggc cgggatcgcc aggaccgtga
     8641 ccgaccggta aatgtcctgc tcgctttgcc aatggcgcaa caaggcctcc gtaacgggcg
     8701 gcaccacggc gtagggaaga aggatcgaga cgctgagaac gccgaaaccc tgggcgctcc
     8761 gaacccgggg aatgaagcag accgccaggg acatgcaggc ggaaaccaaa agaacgaacg
     8821 cggccccgtg aaaaatgcga aggacgatga gccccgggat ggtgaccacc cacccgtagg
     8881 aaaacagggc cgccacgatc atggtgaggg caatcagcat gcccgtggcc gcgtttccgg
     8941 cgtgcagcac ggggaccacg gccaggcgca gggcgaaggc ggccatgggt tccagcccga
     9001 tcaaaaatcc cctccattcc ggggctattc caattcgttc caggtaagtg aaaaagctgt
     9061 aaaagacgga catgttgcag aaagcgaaaa aggccaccag gttcagggag acgaattcga
     9121 aggagaagag ctttcccgtg tccgtcatgg tcggccttgt tcctgcgccg gttcggccag
     9181 tatggaggag agcgcctcga cgaaggcttc catggcttcc atctgggaga tggtgacccg
     9241 aatccagttg ggaaaacgaa atccggtcat ggcccgcacc atcacccccc gggtcatcag
     9301 tttccgatag gccaggctgt cgctcatggg caggcgcacc atgagaaagt tgccctcgcc
     9361 gctgatcacg ggaagcccca gcccgttcaa ttcccgatga aggaacgatc gcccccgtcg
     9421 gaccatctcg agggtgcgct ccacgtgctc ttcatcgtcc agggcggcca gtgcggccac
     9481 ctgggccagg ctgtttaccg agtacacgat gcaggtcctg cgaatgatgt ccaccacctc
     9541 gcgactgccc gccagataac cgatccgcag tccggcaagg ccgtacatct tggagaaggt
     9601 cctgaaaacc accaggttcg ggtagcgggg aaccagggcc aggccgtcgg gaaagtcggg
     9661 atcttccacg aattcccggt aagcctcgtc caccaccacg agggcgcttc cgcccacgcg
     9721 gtcgagaaaa ccgcacaggg tttcccggtt ccaccacgtc cccgtgggat tgttggggtt
     9781 gcagacgaag aggatcttgg tgcgcgggtc gattctttcc agcatgcccg aaacatcgaa
     9841 tccatgatcc accaggggag tcaggcgcgc ctcgatcccc gagaattccg ccacccactc
     9901 gtagacggca aaggtcttgt ccgccgtgat gatgttgtcg ccttcccggc aaaaggcctt
     9961 gatcacaaag ccgatcacct cgttggcccc gtttcccgca aggaactggt ccgggtgcat
    10021 cccaaaccgt tgggcgagct tgcctctcag gtgataggca tccccgctgg gatagaccga
    10081 cccctggacg gcgggaaatc ccatgatggc ggcgcaggcg gtcccggggg ggccgagagc
    10141 gttttcgttg ttgttcaggc gatggattcg ggtgcagccg tagagttgtt tcaacacgtc
    10201 gtccggcttg ctggggatgt aggcgctgaa ggcctttata tgctcgggga ccaggatgtc
    10261 cggagaaaag ccgtccatca cgggcactcc cccggcaact ggaacagcac cacatcgcct
    10321 tctcccgcat agggcaggag aagacacggt cgaaacccgt gtcgaaggag ccccggggtg
    10381 aactctgcct gccagcctac gcccaggtcc aagacaaacc ggatgttgcg gattccttcc
    10441 tcccggagaa gacgggcatg ctgcgcgatg ttttcgtccg catccgcccc gggccacagg
    10501 gggcgaagga tcgcccgcga ttgggtccgg tcgaatgcgg cggaaaggac cgagtgccgg
    10561 ggcagggttt ccccgacgtt gtggaagggt cggatttccc gggggagcac cagcctgcgg
    10621 cattccgtct ccacaaattc catgagctcg ggatgaaccc acacggcact gccggtgtcc
    10681 tcttccagga gccggaagta ggcccgcagg ggcagggagg atccgtcttg ggcatagctg
    10741 gtaagaaccc caaggggctc gaattccccc acgggaaact gcggcgtcgg gtgcatgctg
    10801 accagcccca ccgcccgggt ccttgcaagg gttgcgatgc acccttcgac cagcccttgg
    10861 ggcatgcggg attcaggagg ttggttgaaa acgtacggtc cgaaacattc gaccgtccgg
    10921 gccccggtcc ggtgccagaa gatcgcgccg ccgatctctc cggcgggtcc caccgccgcg
    10981 acggcctggt attcccccac ggcgatcatg tccacgaggc gtccggggga gagaagaaaa
    11041 tccgagagaa aatgccgatc gtaataggat ccggccagga gggaaacgaa ttgcacttcc
    11101 tcgggcgtgg gcggccgcag gacgtatgtc gtgagaggat gggcggcggc cgagaccggt
    11161 tcccggaagc aggggtagga tttctccttg atgagagtca gccgcagttc ccggggattc
    11221 tcgcggccca ggtccaaccg gtccaccgtt ctcgaggcaa ggatcagccc caaccgatca
    11281 aggtccgctt cgttctccac cgacacggag gcagtgaggt tgaaggatcg caggtcgaag
    11341 gcgtcggttc gaaggagaaa ctcggttcgc acgcagtagc cgccgctcga gcagcgaatg
    11401 tccacggggc ccgaggtcga tgtcgccacg ctgcataaat gggaaaacac ctcttcggcc
    11461 ccgagggtga gcccgagggc ttcttcccgg cccaacccca ggcacaggga tgcctgctcc
    11521 acgaagcccg ttaccagggg cagaaacccc ttccgggcgc ctacggtcaa gaacacatcc
    11581 tctgctgcat ctcgtctcac cggcgctcct tatcttccga ttgtccgggc acggagacct
    11641 cgcggacacg atcggaacgg aaagcaaaac ctgggcgagc cgaccttata atcgatcgac
    11701 accaagcatt atccttattc gacgcggttt gcgcaagagt gagttggcct ccaagttgca
    11761 taacggaaaa ggcatcagga cggcacaggc tcctggatcc aacaatagat tgccgcagtt
    11821 gttccgtttt gttcgatggt tgtccaatgg gcccaaagtg tcggaaattt ggcatggaag
    11881 agttgccgac atgggccgtc ccgggatttt tttccgagag gttttggaag catgaaggtt
    11941 ctgttcgtcg cggtcgcgct gtggctctgc ctttgcccac aagcattatc ggggacccga
    12001 atggttccgg tcccggtttt cccttgcgtc gtccagggcc ccaaggcccc ctgccccacc
    12061 gaagatgtgg gaaccatggt ggccttctgt ttcaacgacc gagcccgcag gaccgagtac
    12121 cgggaagtga cggtgaacgt gtcaaaggaa gggggcgtta ccgtctctta tgaactgccc
    12181 ggaagaattg cggccttcca tctaaacgaa ggcggcagga gaaagctggc cgagttgctc
    12241 cgaaagggca ttacattgtg ccggaccgtt cgtgaaagcg agttggaaat gcatagggtc
    12301 ctgggcgtga ccggcgaggg agtcaaagtg tccttctatt cgagcaagga gggcaaagac
    12361 acctccgtcg aggtcgcctg ggtcttcttc accccggatg agacggagaa acggagcgtg
    12421 ccggcacccg tccccgcccc cgtcgaaagg caagacggga gcaagggcaa gagggtccca
    12481 ccgagcaaac aggagaaccc ccaaaagagt cccgaagccg aaccggcggt gccgggccaa
    12541 acccctgtca tgaggctcgg ggcgctggtt ttggccaaag aagacgcgga agggctcctc
    12601 aatctcctcg accgggttcc cgcggcggca aaagccgtca atgccccccg gtacagggac
    12661 gacccgacag ccgattgaac gatttcctcc cccgggacgg cccggcccaa ccgctcagcc
    12721 ggggggaagg ggatttccgt caaaatcctc gtgggccgcc gtgaaatgcc cccgtcaccc
    12781 ttcggtcttt caagcgaatc ctttcctcaa accttcgaac ccatcgtgaa atgctcactg
    12841 ctcagccttc cttttgggct atactggtcc cgcaatcgtt cgggaggaag gacatccatc
    12901 gcgaaatcgt acgccaggga ggaatctatg aagcgggctc tttcggtctt cggtgtcttg
    12961 ggcctttttg tgttgttgga aatcgtcggg ggaggttgga gtgcggccct ttcccgggag
    13021 ggcggaggca cgcccccgcc cccgaaaaag gcccctgcgg tcctgtacgt ggccgatttc
    13081 gagatcgact cggaggacgt caagagcgat ccgggaatcc tggggaatcg gtccggtcgt
    13141 cgggtgggcg tcttgaatcg cgagggaccc ctgcagcgtt ccaaggaccc cgagatcgca
    13201 gcgccaaagc tcagaaatct gctcgccgaa tccttggtcc aagatctggc tgcccattct
    13261 cttaccgccg tgcggctccc tgcgggccga cctctccccc gcgagggctg gctcatccgg
    13321 ggagagttcc tggaagtgga ccagggcaac cgggtcaaac gagcggtgat cgggtttgga
    13381 acgggggcca cggacatgca tgtggaatct accgtgacgg acttgggcgg ggacgtgaga
    13441 caaccgttcc tggtcattgg agtgggagcg ggaagcggaa agtcgccggg ggccgtcgtc
    13501 accatgaacc cctatgcggc cgcggccaag ttcgtcctgt ccaagcacgc atcggaaaag
    13561 gacgttcgaa gcgtggcatc gggaatcact tgggaaatcc tccagtacat gaagaacaac
    13621 gggttgatct ctgcccggcc gtaaagggac gtcgtggcgg atcttgtgag gtgggcggcc
    13681 cacgtccttg tccacaggac ggaaaaaacg gtgaactccg agaccccgga aatcgtcccc
    13741 tgggctcccg gagaggagcg cgacatcgtt tttggggaac ctttccgtcc cgaaacggtc
    13801 aaaaggggtg aagagacctg tccttccttc ttttggcgca accacgagac ttttcccggg
    13861 aggaccgggg ggaccggagc tcccgaggaa atccggaccg ggaatcgcgg gtgctgatgg
    13921 acggttccat gcagcaggaa aaggacgcgg acctcatccg agcctttctc gggggggatg
    13981 aagccgcctt tgacgcgctg gtgctggctt accaggacag ggtctacacc ctctgccggc
    14041 ggcttctggg agaccgggaa gaggcccggg actcggccca ggagacgttt gtgaaggtgt
    14101 accgggcact cgggggtttc cgcttcgatg cggcgttttc cacctggctt tacaccatcg
    14161 ccgtgaacac ctgcaagaac aagctgaaat cccatgagta ccgctggtgg aaacggaggt
    14221 tgcggctgga ttgggactcg ggttccgggg gggagtcccc gcaccgggaa ccccgggacc
    14281 cggccccgtc cgcgttggcc cggttgatcc aaaggggaga cgagatgcgg gttcaaaaag
    14341 ccatggaccg gcttcccgca gaaagtcgag ccgtgttggt cctcaggcac gcggaaggac
    14401 tctcctacga ggacattgtc cggattaccg ggttgaatct ggggacggtg aaatcccggc
    14461 tggcccgggc ccggcaacag ctgcaggaga agttgaagga atcctgagtt tttgcgtcgg
    14521 cagtcgggcg aaagagccaa ggggcgtcaa ccgtcgccgg aacggatctt tcgccggtct
    14581 tgccgggtca tggaggttga cggtggattg cacggatgtt cgacggcgga tgtacgaaca
    14641 gggcgacgac cccatggatc gtcgcctccg ggaggagatg gaaacccatc tttcccggtg
    14701 cgctccgtgt gcccgggaat gggaggactt gcgcaaaaac ttcgagaccc tgggatctct
    14761 tccgacgatt cccgctcccc cgggcttcct gcgggcggtg cgcgagcaga tcggaaaacc
    14821 gtcccgggca ggacgggtta aggaatggtg gacgaagccg tggaaaatcg ggatgccccg
    14881 ggagctggcg ggcctggtgg cggcctcgct gctggtggtg cttctctatc acgggtacca
    14941 gcggattccc gccgttcccg gaaagcccga ggtcggtgcc gggtccctcg ccccgtcccc
    15001 ggttcccctc caggccccga cagccccgcc tccctcgtcc gttctttcct tgcccgagaa
    15061 aaaggagctt cccggggacg tgcagggaat gaaaggggcg cccgagacgg aggtgctgcg
    15121 gctgaacgtg gttcttcgcg ttgccggacc ttcaaagaag cctgattccc cggcgctcgc
    15181 cccggcggaa gctgtcggca gggcggattt ggcgggggag gcccctgcat cgggatccgg
    15241 gaagaccttc gccttccggg agaaggcggc acgaaaggct cccgcccccg gtgcctcgag
    15301 gcaaaggccc ggggcgccta cgccggtacc ggaagcggcc ctcgaggcga aatcggctcc
    15361 agggaaggga ccctcgaagg atcgcatcga ggacgccgtg gcaggaccgc ccgcagaagc
    15421 ccagtccggt ccgtccgaac cggggccgaa ccgggcaggt cttccttccc gggacgggga
    15481 ggaccccgcg gcccggatcg aagaatgggt cctggatgcg gggggcaggg tgatcgaggc
    15541 gggttcccgg gagtccggaa agaacgggcg aatcatccgg gccgaggtcc ccgtcgagaa
    15601 ctatctcttc ctggtgaata acctgtcccg cctgggtgac ctcgatcctc ccccggttca
    15661 ccccgcccgc ggcaaggatc gggaaacggt gctggtcgtc attcagctcc tgttcccata
    15721 gaccggggcc gtccgcaccg tcccgctctc ccgtgttttt cgcccccccg ggaatttgga
    15781 ccggaacttt ttcccctcgt cgttgtccaa tgggttgaaa ggcaatttca aaccccaaca
    15841 accaggaggt tttccgtgga cagagcaggg cgtttcaaag gcaaaaaggc gtggcggctt
    15901 cttccggtct tgatggtgtc ggtgggattc ctgtggggga aaggaatccc cgccgatgcc
    15961 cggaggacgc cgattcaggc ggaatccgcc ggaaccggga cagccgcaac cgaggaggac
    16021 aagactctct ccccgtactt cttcgtcaaa agtgaagatc ccgccgtgga tccactccct
    16081 ctcaaggcca caaggaccgc ggtccaaatt gccggggtca tagcggaagt gaccgtggtc
    16141 caggtctaca ggaacgaagg cactcgaccc atcgaggccg tctacgtgtt tcccgcctcg
    16201 acccgggctg cggtttatgg gatgaagatg accgtcgggg aacgcgtgat cgaggcggag
    16261 atccaaaaga gggaagaagc ccgcaaggcc tacgaggaag cgcggcaaca gggaaagacg
    16321 gcctcgctcc tggaacagca tcggcccaac gtttttcaga tgaacgtggc caacattctt
    16381 cccggggacg agatccgcac ggaacttcgg tacacggaac tgctgacccg gacggacggc
    16441 gtctacgaat tcgtctaccc gacggtggtg ggaccccgtt attccaacga gccggagtcg
    16501 ggcgcccctc cttcggagaa gtggtctcag aatccttatc tccaccaagg ggaggccccg
    16561 ccctatgaat tcgacatcca ggtccgcctg gatgccggaa tgcccatccg agaaatgacg
    16621 tgtcccagcc acgggaccca ggtccggtac gaagggccga accgggcaca ggtgtccctg
    16681 ggtcctgcgg agaaacatgg aggcaaccgg gatttcatcc tcaagtaccg actgtcgggc
    16741 aaggaggtcg aacccgggct tctgctctat gaaggcaaag aagagaattt cttcctgctc
    16801 ctggtggaac ctccccaagc ccccccggaa acgatgattc cgccccggga aaccgttttc
    16861 atcgtggacg tttcgggatc catgcacggg ttcccattgg agaccgccaa gggactgctc
    16921 aaggatctgc tgggcggact ccggccccgg gacgtgttca acgtcctgct gttctcggga
    16981 gggtctcagc tcatggccga acggtccctg cccgcaacgc cggagaactt gcgcaacgcc
    17041 ttggagctca tcggacggca gcggggcggg ggcggaacgg aactcttgcc cgccctcgaa
    17101 cgcgccatga agctcccggc ccacgagggg atggcacgga ccctggtggt cgtgacggac
    17161 gggtttgtcc gggtcgagcc cgaagtcttc gacctgattc gaaactcgct gggccaggcc
    17221 aacgtctttc ctttcggcat cggatcgagc gtgaatcggc atctcatcga ggggatggcc
    17281 cgaatcggga tgggagagcc tttcatcgtg accaagcccg aggaagctcc ccgccaggcg
    17341 gaaaggtttc gacgcctcat cagcaccccc ttactgaccc ggctcgattt caagacggac
    17401 ggtttttcgg ccgacgaggt cgagccgccc cggttgcccg acgtctttgc ccaacgcccc
    17461 gtcatcgttt tcgggaaatg gaagggcagc ccccggggaa cgatttccct gcacgggacc
    17521 ctggggaccc aaacctacga ccggaggctg gacgtggccg cctacaagcc ttccgcctcg
    17581 aacctggccc tgaaatacct gtgggcccgc tcccgaatca cccgcctgtc cgactacaac
    17641 cggctcctcg cctcggaccg gcgcacgggg gaaatcacgg acctggggct gcgctacagt
    17701 cttctcacct cgtacacctc ctttgtggcc gtggacagcg aggtccggcg caaagagggg
    17761 gaatccgcca ccgtacggca gcccctcccc ctgcccgaag gggtctcgga ttatgccgta
    17821 gggggcggcg ctcctgccgg aatagcgccc atgtccatgg cggttgcgcc tcttccgtcc
    17881 cggggccttc ggcagggggt tgaagggatg agcgaggacg ctcggggagt gagggaggca
    17941 aaggaggtct cgggcgtcaa gatgaagaag ggggatatcg aggcccgggt tcgcgtccaa
    18001 agcctgaccg tcgggccggg actcaacgaa tcccaggtcc gctccgtagt cgaagcctcc
    18061 gagaagggcc tgctcgattg tctccgaacg cttctcagcc gggaagtccc agaatggaag
    18121 gtgaaattga cctgggtcgt ggacaaaaaa ggacgggtcc gggatttgaa aatcacaact
    18181 cctgccggga tgacccgtga aatcgaggca tgccttcaag ggcagatctt ggcgtggcgc
    18241 tttgtcaatc gcggggggac ccgggcatcg gcggtgaccc tcgagctttc cgtgcgaccc
    18301 tagacccggc ggggctcatc gggggaccgc cgcgaccctt cccaccccgc ttccctgccc
    18361 tcacttccgg gggggcgtgg gtgggcgtca aactcccgag gcggaccgaa ggccgccttt
    18421 ccttcacccc ggtcctctcc atcgaagcga gggagaggag ccgatgtggg accccaattc
    18481 aacaaccttg ggggccgagg gagggggaat tcatttgcgg aaggaggacc cgcagacagt
    18541 tttccgtccc ctctaaaagt tggacaggtc gtcgtccaac ggtatcagct ggtcggggcg
    18601 tatttcccgg gcctcggttc ggggagagga tgccgggacc atttcccctt ggcggggatt
    18661 cggggcctgc agactgcgcc tcccccgaga tcccggcagc cggggtgaac tcccggactc
    18721 ctccaagggg accggcgggt cctggccccc accccgaccg cccaccaggg aaaccaactc
    18781 gtcgatgaat tccttggttc gttccacctg cccgttgagc tcctgggaag ccgaggcgga
    18841 ctcctccgcc gtggcggcgt tttgctgggt cactttgtcc atttccgcca ccgcgttggt
    18901 cacctgggtg atgcccgtgg cctgttcatg ggatgcggcg gcgatactgc ccacgatctc
    18961 gttggactga gccacggtgg aagcgaccgc ccggaattcg gcatccgtct cttccaccag
    19021 ccgggacccg tccttgacct tcttcaccgt tccttcgatg agggtcgccg tgcttttggc
    19081 cgcctcggcg gagcgtatgg ccaggttgcg aacctcgtcg gccactacgg cgaacccggc
    19141 ccccgcttct ccggctcgag ccgcctccac ggcggcgttg agggcgagaa ggttggtctg
    19201 gaaggcgatt tcgtcaatgg tcttgatgat cttctgggtc tcgaggctgg cccgggaaat
    19261 gtcgtgaatg gagtcggtca gccgctccat ggaccccttg gcgcgctcga cgatgcggct
    19321 ggcctctttc atgaggcggc tggcctgttg ggcatgatcc gcattctgcc gggtcatgga
    19381 ggacatctgc tcgagggacg aggaggtttc ttcgagggat gcggcctgct gggaggaccc
    19441 ttgggaaagc tgccgactta cggaagcaat ctgcctggag cctgccgcca cctgccgggc
    19501 gctctcggtc aacccctcaa tgacccggtt caggggccgg gagatgattc gagagatcca
    19561 gagccccacc aggaggacga ggacggcgcc caagatgccg atccccgacg acaggcccaa
    19621 aatctcccgc acctccttca tgatggccga attgggaaca aagacggcca ggcacagctg
    19681 ggatcgtttc tcgtcggctc cggcgtttcc cgccatctgg gagaaatccc gaacggggag
    19741 atagatgagg tgggtgtcct cccggttcag ggaaaaattg cgaaccgtcc cgccggattc
    19801 ccctttgaac caggactgga gatcggagtc cgcatcgagg gtcttgcccg tttggttttc
    19861 cgcggaatgg gatgcgaaga tgaagtccgc tgtgttcaag acggctgcgg tcgcgtttcg
    19921 caagccctct ttcccgagcc tctccaccga gacccggagg agatcttgaa cgggggccca
    19981 gtcggcgtag gccagaaaat agccgttggg ggttccggac atgccgctga cgcggtgagc
    20041 aaaaaggagg gcccgggaag atttgccgcc cagttgttcg agcaggtcgt tttgtaccag
    20101 ggcaacggag cggggatccc cggcggccag ggcggatgcc tccttgagga cggtgccctt
    20161 gagggattcg gccactttgg cgtccaggtg cgagggggcg gcggcgacct cgaccttgcc
    20221 ctggaggtcc gtgagcacca ggagcgaaaa accgtcctgt gccttgagga aggtctcgaa
    20281 ctgtccctgc acctcgcgat aggtgtgaaa ctcgagggcc atgccgaagc ggtccgaggc
    20341 ggaccaaccg cccgagcttc ctccgatcca gctgttgaag gaggccgccc gggcactcag
    20401 catcccgtcg atttccttgg ctcccccttc acacacaagc tccatcaacc cccggctgtt
    20461 ggccagggcg gccttgcggg cgaaatggta gttgattccc acgatggcga ccatgggaat
    20521 caacgacaac aggagcccac ccatgacttt gagacgcaaa cctgcggaca tgaaccttcg
    20581 aaacatcgct attccccctg gtggtggtgt gttcctgccc gggggacgcc cgggtgcggc
    20641 ctcccctcgg gtctccgttt cggccctcga tcacccccgg ctccccctct ccgccacaga
    20701 cggggtcttc ggcgggggag agaaaggtcc gggggcctgc ggcagccgat cctagaaatc
    20761 cttgaggtcg tcctcgtcca acgggatcaa tctcccgggg tgcgttggcc tgcctgtttc
    20821 cagacgggga gcggatccgt tgcctttggc ggatccgttg ccgggatgtc gggaggaggc
    20881 aagaagcggc ttggcttcct cccggatctt gacctctacc cggctcaccg gggtccttcg
    20941 ggactctgat tcctggatgc tccgggtcac ggcccgaccg atgatccggg ccagttgacc
    21001 cacgaagctc ttcatctgcc ccgcctggcc gctcatctgc tcggaggccg aggcggattc
    21061 ctcggcattg gccgaattct gctgggcgat cttgtccatt tccgacaagg cctggttgat
    21121 ctgctgaatt ccctgggcct gctcccggga cgcggcggcg atttccctca ccagttcccc
    21181 cgacttgatg acggtggaag tcacctcccg gaattcccgg ttggtctttt ccaccagcag
    21241 ggaaccttcc cgcaccttcc gcaccgtccc ttcaatgagg aaagccgtgt tccgggccgc
    21301 atcggcagcc ctcatggcca ggttccgaac ctcatcggcc accacggcga acccggcccc
    21361 ggcttctccg gcccgggccg cttccaccgc agcgttcagt gccaacaggt tcgtctggaa
    21421 ggcgatctcg tcgatggtct tgatgatctt ctgggtctct tcgccggcca gggaaatctc
    21481 ccccatggag caggtcaagg tggccatgga atcgtttgcc tggtggatga tgcgtccggc
    21541 gtcggacatc aactggttgg cgcaggtggc gttatccgca ttctgcctgg tcatggagga
    21601 gatctcttcc agggaggaag acgtttcttg cagggttgcg gcctggaggg aagaaccttc
    21661 ggccaactgc cggctcatgg cggccgcctg ccgggaagcc atggagagct gatcggcccc
    21721 gtccatgaga cccaccgtca cctggtggat gggcctggtg atggccctgc gcaggaagag
    21781 cccgcccccg gagaagagaa ccagggtgat cccgaagagg actcccgcaa tggcgagaaa
    21841 ggtcttgttc gcttgttgga tttccgtgtc caggggggct cgaatggaaa tgatggcccg
    21901 ggtctctccg atcttttctc cgaagccgct cttgtccccg tagcgatcca gcaattcctt
    21961 cggggctacg tcgggagtgc tgtggcaacc catgcagtcc tgggtgttct tggtgaacgg
    22021 caaggcgtag taaaggtacc gattgccctc tttctcgatg acctgcttat actcggtcat
    22081 gtccccggta ttgaacctcc ggatgaggtc ccgttcgaat tcgtcgctct tgttgagtgg
    22141 gtttcggggg ttgtttgccg ccagtttgta atagaattcg ggaagttggc tcttttttct
    22201 caggtcgttg taatactcgt gcaggttcct ggtgatgtac gaagaggaca aaatttccgg
    22261 ggaaaagtat tccttaggaa gctttccttc ctccttgagc tttccaatgg ccggtttctg
    22321 gtgcttggcg atgtaggcgt gaagggcctg gtgttccagg agcaggttct tgatcttctt
    22381 ttcggcctcg ccgatggcgt aggattgagt ctggtagtaa aagaaaacgc tgagaacgca
    22441 ggtggtcgcc acaaatatcg cacacatgac ccacaacatg gtacgtccga tactggtggt
    22501 cttctcggtc tttccggaaa acatcagtag cctcctcatg aagaacgtcc acacatcttt
    22561 cgccaatcga tccggaatct cggtcaagag ccgctgcgcg ccagcatctt tcccagaacc
    22621 gctccgacct cttcgaagct ctcgtccaca aagctgagga aacggggata cagggtgtgt
    22681 ccccgggcaa tggtctcccg ctccctgtcg ggaaggagca ggaggatgcg caaatcggaa
    22741 atgaggggac ccagggagac gatttgcgag agctcttccc ggctcgagca caggattacc
    22801 gccactcgaa gctctccgcg gggacggcgg aatcggtcgg agaggctctt catgtcccga
    22861 tggacttccg tctcctcggg aggaatccgc ttgtagacgg cttccaggag ctttcttccg
    22921 gcagtctcgg aatcggagaa atacagcagc agtctcaagg cagcgaactc cgggtctatt
    22981 gcctccggac ggggccgaac ggcattgggc cccgttgcct tccaggcgtg gacggggtcc
    23041 tggaagagtg ttgcccctgg ccgtcgagtc cccgttttgg ggatcggatt tccgggtgtc
    23101 accggcatcc gcggcttcgg gacccgggtc cccggtgaaa gaccctcccc ggtcgaacca
    23161 ggggtagagg caaaggtcgt gccgaggggg tttccaatcc gggccatcaa tcgggtccta
    23221 tctccaaggt gtgccgaagg cgggagcgag gttggttcgg cttcttcaac gagagggacc
    23281 cggccctcgg gaactgcgga gaagaaatgt tcggaaaaag tgaacacgga gggagggcaa
    23341 gagagagaaa acttgcgggc agcgggcaac ggagggcatt tcaaaaaaac cggaccttcg
    23401 tccggaaagt ccggacactg agggaggccc ttatcggtcg gaccgggaaa ggccgtgttt
    23461 tttcaagagc atgtacaact gggtcctccc cagcctggaa acccgacacg cttccttgag
    23521 gttgccgtgg gtgtattcca gcaggttctc gaaatacttc ctttcggcct cctcgaggac
    23581 cgaattgcgc aggtcccggt agctggggaa gtccttggaa tccatgctcg attcccggga
    23641 ttcccggccg ccggaggcct cgcctttggc catggccgac ctcatcacct ggacgcggat
    23701 ctgggtcgga aggtgtttca ggaaaaggat gggttcgtag cgtgccgccg aaatggcgct
    23761 ttcgagggca ttgatgagct cccggacgtt gccgggccaa tggtactggg tgatcgcctc
    23821 gaggaagtcg ggagaaaagc ccttcccctc gatatcgtaa ttcttgcaga gcttggacag
    23881 gtaatggaac gccagttcct ggatgtcttc ggggcgattt ctcaaggggg ggagttcgat
    23941 ggagatggac cggattcgaa agagaaggtc ctttcggaac aggccctcct ccaccattcg
    24001 gtccaggttt cggttggttg cggcaatgag cctgaaatcg ctccgggtct cctccttgcc
    24061 ccccacggct cgaaaccggc gctcttgaag gacccgcagg aaggtcttct gaacggagag
    24121 gggcagttcc cccacttcgt ccaggaacag ggtcccttcg ttggcctgtt tgacaaggcc
    24181 ttccttggcc cggtccgcgc cggtgaaggc ccctttctcg tgcccgaaca gaaagctttc
    24241 cactagggtt tcggggagtg cggcgcaatc gaccaccacc aggcttcggt tcctgcggga
    24301 gctgttgtcg tggatggccc tcgagaagag ctccttgccc gttccggttt cgccggtgat
    24361 gagcacgtgg gcgtcactgt tggccgcctg ggcaacctgg tccaggcagg ctctcatggg
    24421 agcgctgttt cccaccaatc cgtcaaactt gagggtgacc gtctgtttcc ggcattcccg
    24481 aaggttttcc cggtactgga agacccgttt gaggggaaga aggatggtct gggccaggaa
    24541 gggtttctgg atgtagtccc aggccccgct tttcacggcg agctccgccc cgtctgcgtc
    24601 tccgtagccc gtgatgatga tcacttccgg tgaggaggcg gtctcccgga tccgggggag
    24661 caaatccagc ccgttcccgt cgggcaggcg cacgtccagg aacacgacat cgtacggtgc
    24721 ggccagcagt tcctccatgc cccgggcaag ggtgtgagcg catgcggcct cgtggcccac
    24781 gcgcctcacc agttcgcgaa gcagctcgga cgtatcggga tcgtcgtcca caatgaggac
    24841 tcttgccatg atcgaggtcc tggtttccct gattcttccc ggggagctcc cgaacggggt
    24901 gggagagctc ctctccacgc atggaaccgg tcatcgtttc cgaacgccgt cgcgggggca
    24961 agaccccgac cccatcgtgg tgcgtgaccg ctgagggaat aataggaaag tcgaaacgat
    25021 tgctcaacct ttttcgggcc cggatcaccc ccttctttcc cggctctcct ccggcgggag
    25081 actcggttcg gacgcgctca ccggcgggcg ctgccgtgga acgccgcttc ccggagaaga
    25141 gtgcccgcgg tgccgcttgc cggaaaggtt cgtcacggtg ccagggcttt tcctcggcgt
    25201 cgaacccggt gcgggagggg ggccgttccg gaccggtttt ggacctcggc gaacccgggg
    25261 aagggtcccg ggcaaagaaa accccctccc tcccgggagg agggagaggg cagggggggg
    25321 gaaaacaaca cgatcctggt taagcaagat cgatgccgga atcggaggac tcgaccgatt
    25381 tctctcttcc gccggaaaat tagggagaag gatcgagaaa ggaggaccgc cggacccccg
    25441 ggccgctttc gaacggcttg atcccgaaaa cgggagcggt taggatagcc gcgttcatcg
    25501 tatgtctttc cggtcgggag cgaagatacg gcagccctgc ttcccaccgt cgtccgcgat
    25561 tttgccgtcc ccggaagaag cgggccgttt ctccggggcg cggaaatcca agaggacttt
    25621 tatggatgtc gtcctgttgt cgagatggca gtttgcactg accaccgcct ttcatatcct
    25681 ttttccgacc ttgaccatcg ggttggcctt gtacctggtg gtcgtcgagc ttctctggct
    25741 gtggaagaag gaagagatct actaccgact gtaccggttc tgggtgaaga tctttgccat
    25801 tcattttgcg gtaggggtgg tgtcggggat cactctcgag ttcgagttcg gcaccaattt
    25861 cgcccggttt tcccaggcgg tggccaacgt cattgcgccc ctcatggcct atgagggcat
    25921 gacggccttc ttcctcgagg ccgggttcct gggaatcatg ctgttcggat ggaaccgggt
    25981 tccccggggg atccatttcc tttcgacctg cctggtggcc ttgggcgccg ggctttcggc
    26041 cttctggatc atggccgcca acgcctggat gcaaacccct gcgggctatc gctttgccga
    26101 gggtcgattc tacgtgaccg attttctttc cgccatcttc aatccctccc tgcccactca
    26161 tctcagccac atgctgctgg cttcctacga gacggcggcg ttcgccgtgg cgggcatcag
    26221 cgcctattat ctcctccggg gccgaaaccc cgcgtttttt cgaaaatccc tttccctggc
    26281 cctggtgatg gcggccgtgg cggctcccct ccaggtcctg gtgggggact tcaagggcgt
    26341 ggtcgtggcg aaacaccagc cgcccaagtt ggcggccatg gaggcccact ggaataccaa
    26401 cacccggggc ggggcccctt tcgtcctttt tgccctgccg gacatgcagg aagagcgcaa
    26461 tcgctgggag gtgaccattc cccacgtcct gagccttctc atcacccgta cctgggacgg
    26521 aaaggtcacg ggcctgaagg aatttcctcc cggggaccga cccaacgccc tcatcacctt
    26581 ctggtccttc cgactgatgg tggccgtggg tttcctgtac ctggccctca tggggtgggc
    26641 cgcctttctg tggcgccgca aaaggctcta tgattcccgc ccgttcctgg ttgccctagt
    26701 ggcggttcag cccctggggt ttgcggcgac ggtatccggc tgggtgaccg cggaagtggg
    26761 caggcaaccc tggctggtgt acggaatcct gcgcacggcg gacggagtct cgcccatcgc
    26821 ggccggaaac gtggtctggt ccctgttgct cttcctgggc tttttcagtt tgatcgggac
    26881 cagttatttc tattacgtgc taaaaaccct gcatcgggga ccggacttct cgagcccgat
    26941 tcccccgatc caaagaccgg cgggatttcg cccgatccaa ggggtcgccc aagggacgga
    27001 ggaaagctga tggacctggt gaacctttgg ctttgccttg tcgggcttgt gatccttctg
    27061 tacgtggtgc tggacggatt cagcctggga gtggggatcc tctttcccac cgcccgcaac
    27121 gaggccgagc gggacgttct catgaacagc atcgcccccg tctgggatgc caatcaaacg
    27181 tggatcgtct tcgggggcgg ggccctcttc gccaccttcc ccatggtcta ttccgtgctt
    27241 ttcagcgccc tctacatccc gctcttgacc tttctcttcg ggctcatctt ccggggaacg
    27301 gccttcgagt tccgggcgaa ctccacgcga aagtctccgt ggaaccgagc cttcttcgga
    27361 ggcagcctga cggcggtttt tgccctgggg gtcaccctgg gatcctacct ctcggggacc
    27421 cgggtggtga acggccagtt tgcgggaggg gctttcgact ggctcaatcc tttttccgtg
    27481 atggtgggcc ttgccctgat cgggggctac gggcttttgg gagccactta cctggtgctc
    27541 aagaccacgg gtccggtcca ggagcgggcc taccgccagg ccttcttcct ggcgtgggtg
    27601 gtggccggtt tcatgctcct ggtgagcgtc tggacgcctc ttcacgaccc ctccatcccg
    27661 gcccgatggt tcagcgttcc gcgggtgtat ttcatctggt cctttcccct gctgggggtt
    27721 ggggctttcc tgacgctgat tcgcagcctc cggaaacggc gggagttgct tcccttctgg
    27781 agcagcgtgg tcctgttcgt ttccgcctac ctgggactcc tggcggccgt ctacccttac
    27841 gccgttcttc ccggcgttac cgtctacgag gcggcggccc agagggaaac catgctgttc
    27901 accctgtggg gagtgtgcct ggtgctgccc gtggtcctgg cctacaccgt gtacagctac
    27961 tgggtgtttc gggggaaggt tgtggcggag gaagggtacc actaagcttc cgcggcaccc
    28021 atcggacaac cggggctttc tgccttcctg ccgatgaacc ggggatccgt gcctcctttt
    28081 cgggttgggg tttggggatt ggactccccc tgccgagggc aattgcgacg ttcctccctt
    28141 ccggaatagg gctccggccg caggatcccg gaggaatccc ggcatcggca cgagatccat
    28201 gtggaaggga gggcgcccca ttgtattgta cgcaatcttc gcccttgacc ttgccgacct
    28261 ccaccgggag gactgcacta ggttcccgcc cgatttgagc gattcggaga gcatcagccc
    28321 gtcatccccg cgaaggcggg catccagaag gcatacaggc cctttatgga cgctcgcttg
    28381 cgcgggaatc tttcatgcac gagggcgccc ccttggatga aacggtgctt tgccgagcag
    28441 gtagggtggg cacgggtttt gtcctgccca ccgtctgggt ccgcaaacgg ggagcgatcc
    28501 cggcagggaa tcgctgcagt ttcttcataa acaagctgcc cgcttcggca aaaaactccc
    28561 gggggagaaa agcagagcct tcccgatatg aggcgattga accacgaagt gaggcgggcc
    28621 ggggtagtgt caacaatttt gtgtaaattc atcacgccgc ctcctacttc aaaagaggca
    28681 ggtttttacg cactgttcgg gccgaagtac gcagacgaat acccgtaaac atctccacgc
    28741 tgataggccc tcgggagaga gcctaacccc cgaaagaccg tttcagggcc tcgtagatct
    28801 ctctttgaac cagctttggt gctcgtcgga ttcttgcgag gatgtttcgc gcaccctcgc
    28861 tgacgggctg aagattggcc ctgctctggc tgcagatcga acagaggacc cgccggttgg
    28921 ccttctcgtt ggttcccccc ctggctcagg ggtgtcacgt ggtctatgtg aagggacacc
    28981 tttctgctgg ggttaaaggg atcggggtct ccaggccctg ctccgcagtt ttggcatgtg
    29041 taaccatttc tctccaggat ctcgttgcgc aattggatcg ataccgccgc accgaacgta
    29101 gttctgcgct ccgacgtttc cagtatatat tcaccgggct tgaggtcatg acggtctttg
    29161 tgggagctga tttgcatccc ctccaggtct ctcaactccc gaattcgccc ggcatactcg
    29221 ctaattccgg caacctcccg gatttcctgt gtcgtgacga ccctgccgac gttggcttcc
    29281 aagaaagccc tgatcctatc cccggcgctt gcccctcgta ccgccaatta tttagaagct
    29341 cccgcagtag gaatgtctcc gttgtttctt tccggtcatg caatcccaaa tcaccagacc
    29401 gtgtggaatg cttccgaccc ttctcgccgg ctttggtatt gccgcctctt accttggtta
    29461 tggatgtttc ccccttcagg catgggcctg tcggacatcg tcccgttctt ctgagctccg
    29521 gatcagttcg gttacacagt cggcgagtct tgccgccagg aggggaggga ccgcatttcc
    29581 gatttgtttg gcgatttcta ttttagatcc gcagaaacgg aagtcgtcgg gaaatgtctg
    29641 gagtctggca gcctctcgat gggttatcgg tctgtgttga ctcggatgaa ggtagcgacc
    29701 cttttcgggc ttgaagaatt ccgtcctgat ggtgaatgcc ggcctatccc accacaatct
    29761 gccgaacaaa tcggtccctc cggactcttt ccggatccaa cagtcgggag tcagttcggg
    29821 tgccagacgc tggaggtcga ttcgattcat gccttctttg gataccgtct tgtatcgcct
    29881 aaggctgata gcggtggggt tcctgccgaa atgcagatcc aaaggaggag aatcctccct
    29941 gatgtgaacc ccgacgggag gaggaagatc ggctatggcg tcccttacgg tccgccaggg
    30001 aaggggattg gggacgtact ccgactccga agacgtaccg ttggagggat tacggtgagt
    30061 tttcctcggc gggaagaacc gtgtaggatc cccgatgcgg ctccccagaa tgaaggcccg
    30121 ccatctggtt tgaggaacac catagtcggc ggcgcacaat ttggcccaag tgcagacgaa
    30181 gcccagcgct tccgcctccc gaacgatctc ctcatgttcc ggcgaaccca ggagctgcgg
    30241 gacgttttcc ataagaaaga ctcgggaccc cgtcttctgc acgacctcca ggaacggtcg
    30301 ccatagttgc ttgcggggat cttgggtgcg ttgtttgttg agaagactga atccctggca
    30361 cggcgggcct ccgatcacca cgtccgcgaa aggaatcctt ccatcggcgc gggatagaag
    30421 ggaaaggatg tccccgtgaa tgcagtgctt cccgaagttc ttgttgtagg tttgtactgc
    30481 ggactcgtta gaatcgttgg cccatatggg caggaaggaa tgaccgaatg atttggtaaa
    30541 ccccagagtg agacccccgg cgccggaaaa gagatcgacc agtcgatatt ccctattgtt
    30601 catgcgtcct tccgcaagac ccgttgacca tgctctttct catacccata cgacccggcc
    30661 tatttaaacc acattcgggt agggacatca actctcacga tgctgcagct tcgccgctcc
    30721 gttgtcggct tctacgccgt catttgccta ttgtggagtc ggtcgaacgt tggggctgag
    30781 ccgaggcgag ccccagcact gcacacttcc acggcacccc gtgtggcccc ctttggctcc
    30841 ggtccttggt tgggcaattc gcctcagcca agtagccggg taaggtgggc acgggtcttg
    30901 tcctgcccgc cctacggctg aaggcgggag cgggtccaat gacatcggcc gccgccgggt
    30961 cctcgaactt gcctgcaacc gcaagcaaga cgaagaattt ctcttcgctt ttcgggagac
    31021 gaaagggcgg ttcttctgcc cgtcgcggcg gtgccgggtg cggaggcggg cttcggaggt
    31081 ctctctttga cgggcatggc aggatcgtcc tcgccaaatc gaggaagtcg tttccaaatc
    31141 catcccgtca cgggctcaaa cagtggaatc caggacaccc gccaaagaga agaaagtcgt
    31201 gccgggggca ggtcttttga agacccggcg agccgggcgc ccgcgcatca agagggcccg
    31261 gtctcgcccc tgcggggaag gcggatgaaa gaggcaagaa cccccgccag caccaggagg
    31321 gtgcccacca cggagatccc gtgggcaaag gagtccgtct ttcccaggat cccgatgagg
    31381 gtgggggccc cccccgctcc caccaggaaa gccatggaga tgatcacgga aatcaccacg
    31441 ttccgggact ggggaggacc gatgagggac atggctgcca gggcggcggg aaagaagcac
    31501 acggaaaggc ccgcctgaac aaagatcacc catacgatcc acgaagaggg ggccaggccc
    31561 aggagcaggg tgctcagccc cgtgagaagc aggctcacgg tcatggttcg tcggaggccg
    31621 aaacgatccg aggcccaccc tcccgccagg gccagggcgg gcgtgggaat tcgggacaag
    31681 gccaggaggc tgttggccca gctctctccc atgccctgtt cggacaccag gtagaggggc
    31741 agcatcgagt aaatcccctg ggacccgctg attccgatgc tgaacagaag aatcatgatc
    31801 cagagagagg attggcccag caaaggcctc aaggcgccga aggacggctt ctgcccgggg
    31861 aaatccccgg cccgggagaa ccgcgagaag gccaggccca gaagaaggga ggcgcccccg
    31921 accagtccca ccatggcgcg ccaggaagcc cgggagagga ccagttccga aaacagcggg
    31981 gcacagacaa aggccagatt gggggcaagt tcgtgaatgg agatggcttt tccccagtgc
    32041 ttgggggcta ccagagaggt gatggaggcg attcccgagg gaagatacag gcccgcggcc
    32101 atgcccatga gaaagagccc cgcgcgaagg ctcgaaagac tcccgctgag gctcgtgccc
    32161 agaaacgtga tccccaaggc cgcacaggaa agaaggatgg tcttgcgatg gcgcagccgg
    32221 tgggacacga acccggacgt cgtcagggag aggaaatacc ccgaggcgat caggagaaag
    32281 agggatccgg cctgcccgtg gtccaggttc aagtcctttt cgatggtggg catcagggga
    32341 gaagggatga tgcgggcaat gaagttcacg aaaaagatcg ccgtaagaaa caggatgggg
    32401 cccaggtgcc ggcggaagga aagaggatct gttcccggtt ttcccggctc gtcgtcgatc
    32461 ggagatgggt tgatcaagga cttcctttcc tggtaaagat tgctctcgtt aggacgggga
    32521 agtgtagggg aacggcgacc ctgcgtcaaa agaaacttca aaggaaggac ccctcatgca
    32581 gcgagatccg aaggatccgc ttttggtgtt tccccggggc agtttctcgg cgcggttcgt
    32641 ccggcgggaa aagcgctttc gggtggaagt ggagacgcag gaagggcgca cttgggtcca
    32701 ctgcaacaac tcggggagca tgctgggcct cttgagaccg ggctctccgg tgctggtttc
    32761 ccccgccccg ggccggggcc ggatcttggc ccataccctc gagttgatcc gggtgggagg
    32821 aatctgggtc ggagtcaaca ccctggttcc caatcgagtc ctgagggtgg cctgggaaat
    32881 gggtctgctc cccgagctcg aaggctgcga ctccttccaa aacgaggccc ggtccggaga
    32941 cagtcgcctg gacgcccgca tccggggagc ggcgggaacc gtctgggtcg aggccaagaa
    33001 cgtcaccctc gtggagaacg agaccgccta tttccccgat gcagcgacga caaggggtca
    33061 aaaacacctg cgggaactgg cggcattgac tcggtgcggc gaccgatgcg cctgctttta
    33121 tctcatccag cggcaggacg cccgctgctt ttccccggcc gatttcatcg atcccgcctt
    33181 cgccgagatc ttctggaaag ccctcgatgc cggagtggag gtctggccct accaggcccg
    33241 ggtttccccc gaggggatcg gactgggtcc tcgattgccc ctttcgaagc gttgaagggg
    33301 tcccgccgcc ccggggaccc cttcgaggtt tctcttgttg gtccggggga aatgtgcgaa
    33361 gaacaacctg tgtccctggt caacgatcga gggcgattgc cggggcaacc gagtactcgg
    33421 cctcgagtgc ttccgcccgg gactcgtgca gcaggatatc ccgcgtgaaa ccttcgaata
    33481 cgaaaagctg ggccgtgttt cgtgcagcga tcctctgcct gatgctcgcg gttcccgccg
    33541 tcggttggtg cagggagagt tccttgcccc gggaagaggc cctgaccctc gaccaggccc
    33601 tcgccatggc catggaaaag aaccgcctgg tccggagcgc ccgactggaa gccgataagg
    33661 ccgaaaacaa ggtggcggaa gcccggacga acctcctgcc gattctcagc ttcgacctgt
    33721 tcgagatgta tgccctgacg tcccagacct acaccttcga gcggggcctc ctgggcacca
    33781 ttcccaacct gggtcccatc ccccggaaga gcacccgcgt ggacatcagc ccggaccttc
    33841 tcagcatcgt cagcctcacc gccacccaac ccatctccca gctctacaag atcgccctgg
    33901 gaatccgcca ggccgtggtc ctgcgcgata tggcccggga ggaactccgg gcccggagac
    33961 agtccgcgac ctacgaggtc aagaaggcgt actacgggat cctccagtcc caaagcgccc
    34021 tcggggctgc ggaagacacc atcaagctcg accgggagct ggatcggttg acggacgact
    34081 acctcgccca gggaaaggtc ctcaaggcgg acagcctggg cgtcaaaagc cgcctggctc
    34141 ggtcggaaca cgaggcgttc agcctgcgca atgccctggc gacccggaaa gaacagctca
    34201 acgatctcct gggacgagac gtcaagaccc ggttcagggt ctctcccgtg ggagacctca
    34261 ccctcttcga ggcggacctg gatgccgcgg aagctcgagc cgtcgagcag cgtcccgagg
    34321 tgcgggaggc acggctgaag gtcaagcagg cggaatacga aaagaagatt aaaaaggcgg
    34381 aatatattcc ggaccttagc ctcatggcga actatttttc ccccgtcggt tcccggttca
    34441 ttcccgataa tgtggccgtg gtggggttgt atctccgctg ggaattcttc gactggggtc
    34501 gcaagcagaa ggagctggtc cagagggcgg atgccgtcga gcaggcccac cttgccctgc
    34561 gcgaggcgga aagcaaggtc gtcgtggatt tgaacgccca gtaccgaacg gtccgggagg
    34621 cccgctccct gctcggggtg tgcgaactcg cccgggaggc tgcccgggaa cagctgcggg
    34681 tttccctcga gcggtattcc cgggacgcgg ccctcgtcaa ggacgtcctg caggcccagg
    34741 ccgccttatc cgaggcaaac caccggtatg aggagtccct gttggcttac tggaccggga
    34801 gggcggactt cgagagggcc ctgggggagg agtgaatgaa gacgagagcc tgctggattt
    34861 ggatctctct cgcgcttgcc tggttgggcg ggggatgcgc ccgggaggag ccctacaaga
    34921 agcccctgac gccggtgcgg gtggaagaag cgagaatcgc tacgggagac ggcggcgttc
    34981 gttactccgc cgacgtgcag ccttacaccc aggtgaacct ggccttcaaa gtcgcggggt
    35041 acgtggagtc catcctgcag aagcggggag cggacgaccg gatgcgaaac ctccaggaag
    35101 gggatttcgt ttcggccgga accatcctct cgacggttcg caaagaggat tacctgcaga
    35161 aggtgaagga ggcgaaggcc caattgtccg ccgcccgggc cttgctcgag aaagcccgcc
    35221 gggacttcga ccgggccgtt catctgctgg caacccggag cctcacccgg gtggattacg
    35281 acgcggccca ggcccagttc gaggaagccc gctcgagggt ctcgggggcc ggggctcaag
    35341 tggacgaggc cctgattcac ctgggacact gcgagctcaa ggcacccatg gacggggtcg
    35401 ttctcaagag agaggtcgag gtgggctccc tggtcaatcc ggggacggtc gccttcgtcc
    35461 tggcggacct atcgtcggtg aaagtggtct tcggggttcc cgaccagatg ctgccgtacc
    35521 tgaagctggg ggattccctg gcaatcacca cggagtccat gccggataag gaattcaggg
    35581 gtttgactac ggccctgtcg cctttggcgg accccaagag ccgaatcttc aacgtcgagg
    35641 tgacggtccc caatcccgac aatctcctga aagcgggaat gatcgcggcc ctccaggtgg
    35701 cgcgacccga ccggccccga caagttccgg tggtttccct caaagccgtg gtgcgttccc
    35761 ggacggaccc cgggggatac gccgtcttcc tggtggaggg gcgggaaggg gtggcgacgg
    35821 cccgcctgcg caacgtggtc ctgggagacg tggtcggaaa caagatcgag gtcctggaag
    35881 gaatccagcc gggagacctt gtcatcgtga cggggtccac cctggttgcc gacggcgaat
    35941 cggtgcgggt gattccttag ggttgcgagg tccgggggat gactcataag agcgacgcgg
    36001 aaatcatccg ggaaacccgc aataccgccc ggtattttgc ggagaaccgg catatcgcct
    36061 gggtgcttct cgtggtggtg atcctctggg ggatctacgg gtacgtgaat atgcccaagc
    36121 gcaaggaccc cgacatcccc gttcgcctgg cggtggccct gtgtccctgg ccgggagtcc
    36181 acgccgaaaa gatcgagcag ctcgtcaccc gaaagatgga agccaagatc gccgaaaact
    36241 cccgaatcca tcctcccagc gattacgact tcggcattat gtccatctcc ctgaacggtc
    36301 tgtccattgt ctacgtccag ttgggcgaag acgtggaaga caccaagaaa gagttcaacg
    36361 acatccacct caaactcgac ggcctcaagg atttgcccga gggggcgggc cccatccggt
    36421 ttatcagcga ttttggggac acggcggctt tgatgctcac cgtggccagc cccaaggaag
    36481 gccccgacga gatcgccctg cgggcccaaa gcatccggag agccatccgg ggggttcggt
    36541 ccggggccgc cgcggatggg cgagggtccc gggccacgct ggtggtggct cttccctttg
    36601 caacgaaccc ccaggtcctc agccgcgggt tgcatctttt cggccagtac atgaaggaga
    36661 actccctggg ctgggacctg cgccccatcc agggatcggg gttcctggcc ctggatttcg
    36721 ccacctgtgc cgaagacgag gccgttttcg atctcgtggg caggttcctg cgggagagga
    36781 ttcacgaaga agaggttcat cccgacgcat ggatgcccgt catcgtccgg gatcccgagg
    36841 atacggaagc gaaactggcg gcggtggccg ccgaaaaata cagctaccgg gaactggacg
    36901 atttcacgga cctcatccag cgaaccctcc aaccggtccc ccaggtctcg aaggtggacc
    36961 ggaccggggt cctgggtcag gagatcctgc tggaatattc ccaggaaagg ctggcctcct
    37021 acgggattca gccctacaag atcggggaca tcctccgggc ccgaaacatc aacctgcccg
    37081 ggggagtcct ccagatcgag ggcaaggaga tcggcatcga cccctcgggg gagttcaaga
    37141 acgaaaagga aatcggcaat gtgctcatcc ccgcttcccc ctcgagcatg cccctctatc
    37201 tccgggacct ggtggatatc caccgaggct acgagagccc tccccgcttc ctgaactact
    37261 acagctggcg ggatgcccgg ggggaatggc atcggagccg ggccgtcacc ctggcggtgc
    37321 tcatgcgctc gggcgagcag atcggggcct tcggcgctca aatcgacaag gccctggcgg
    37381 aggtcaggca gcaactgccc gaagacctca tgatcgtccg cacctccgac cagccccggc
    37441 aggtgagcga ggaagtgtcc ctgttcatga aggcccttta cgaggcgctc attctggtgg
    37501 tcctggtggc ctggctgggg ttctgggaat ggcgttcggc gctgctcatg gccctgtcca
    37561 tccccctcac cctggccatg accttcggga tgatggatct gctgggggtg gacgtgcagc
    37621 aggtatccat cgcttccctc atcattgcgc tggggctcct ggtggacgat ccggtggtgg
    37681 cgggggatgc cgtcaagcgg gacctggccc tgggacaccc gtcccttacg gcttcgtggc
    37741 tgggtcccac caagctggcc aaggccatcc tgtacgccac catcaccaac attgtggcct
    37801 atcttccctt ccgacttctc agcggcgata ccgggttgtt cctggccacc cttcccatcg
    37861 tcatggcctg ctcgctgatc tcctcgcgcc tggtgtccat gaccttcatt cccctgctgg
    37921 gctattacct gctccggccc ccccgcaaac ccgagccttc cctggaagag cggcgtaccc
    37981 gggggttcac cggcttctac taccggatgg ggagcgccgc catccgtcac cggtgggggg
    38041 tctttacggg ttccctggtc tttctcgggg tgggcgccct gtttttctcc cagctcaaga
    38101 cttctttctt tcccgatgac ctccagtacc tgtcctacgt ggacgtgtgg cttcccaacg
    38161 acgcctcgct cctcaccacc aacgaagccg ccgtccaggc cgaacgggtg gtggaggagg
    38221 tggccgacga gtacggccga acccacccgg gaaaagacgg caagccccgg caggtcctca
    38281 agtccgtcac caccttcgtg gggggcggcg ggccccggtt ctggatctcc gtttcccccg
    38341 agctccaaca gcagaactat gcccagctca tcctcgagat ggaaaacaag gaggataccc
    38401 ccctgctggc gaggcccctc caacaggccc tttccgcgcg gattcccggg gcccgggtgg
    38461 acgtgcacca gctccagatc aacccggtgg attttcccgt cgaggttcgc atttcgggaa
    38521 gagccgacct gggccttcgg aaggacgacg cggacgttcg ggttcttcga aggctgtccc
    38581 acgaggtgaa ggagatcttt cggagcatcc cccaggcgga ccgggtccgg gacgactggg
    38641 acgaggagtc cttcgtggtg agcctccagg tggacccgga ccgggccaac ctggccggga
    38701 tttcgaacct ggacgtggcc gggtcctgcg cttcggccat gaacggaatc cccctcacca
    38761 ccctccggga aggagacaag gaaattcccg tcctcgcccg gcttcgcatg gaagagcggg
    38821 cccgactctc ggacatcaag agcctctaca tctactcgat cgagaacgcc aacaaggtgc
    38881 cgctcctcca ggtttccacc gttcactccc agatgagaac ggaaagaatc cggcgtcggg
    38941 accatttcag gacctttgcg gttcattgct ttcccgtccc cggagccctg gcctccgagg
    39001 tgctcgaaca ggcctatcct cgcctgaagg ccctcgagga tcgacttcct ccgggataca
    39061 gaatggacat cggcggggaa caggccaagc aggaccaggg atttgccgag ctggcgatgg
    39121 ttcttgccat ctccgcggcg ggcatctttc tcgccctggt gttccagttc aacaacgccg
    39181 tcaagccctt cctggttttt gccgcggtgc cctacgggat ggtgggcgcc ctgggggccc
    39241 tctacgtcat gggcgccccc ttcgggttca tggccttcct ggggattgcg agcctggtgg
    39301 gagtgattgt gagccacgtg atcgtgctgt tcgacttcat cgaggaaatg cacgagaagg
    39361 gcgagcccct gagggaatcc ctgctggatg cgggcatcgt gcgcctgcgc ccggtcatga
    39421 tcaccgtggg agccaccgtg cttgggctct tccccctggc cgtccacggc ggccccctct
    39481 gggaacccct ctgctacgcc cagatcgggg gactcagcgt cgccaccttc atcgagctca
    39541 tcctggtgcc ggtgctgtac gccatcttcg tcgaggacct gaagctggtc cgatggaagg
    39601 ggaccgggga gccttcctcc tgaccgtaga ctgcagggaa gcggggccgt ccccgggacg
    39661 gacacacatc ttgggaaacg atcacaaacg agaactcgac cccaggggaa ggagcttcat
    39721 ggaggacagg accctcagaa tcggtcggtt ctttaccgac actcgaatct tgcgatgctt
    39781 tgcctccaat tgtcggttcc acttaaaaga ctgctgcaac ctgaaacagg tctccgtgga
    39841 tcccgaggcc cgttgcaccg ggtatgaacc ccgaaaggca gcgcttccag gggaatccaa
    39901 gctcgagatg gaaaattcgg actgacgggt ggaacggcgg aaaacgaggc tcggggcgga
    39961 acttcccggt ccggtgggtt tttcgaggcg gagggcggga ccgtggccgc cgtccgggtc
    40021 ccaatccgtg caagatccga gagacctttt gcccgagccc cggccgtcga gagtcggcat
    40081 gattcgagga aggaagtggc cggttcgaga cggggacgaa cccgccgcgg gagcctgccc
    40141 ctgaaaaagt tttgcctctt ccacaagccc ctgctctcgt ggaagaggca ctcaggaggt
    40201 aattatgcag tgaacgtacc ttagaacaga agtttttgtc tgtcaaacgt tttttgtaca
    40261 ttgcgcacaa tttgcacaga atctcccgcg ccttccttcc tgccgcagcg gatctccccg
    40321 ggaaaccctt cggattctcc cttccccggc cccgaggatt cgattcttga atcggggagg
    40381 aaacccctcc agaatgagga ccttgaggcg acgagggccc ttgaaatcgc atccgggccc
    40441 gggtttccgg cctcgagagg ggggatgggt ggggatgtca acggagtgaa ccatggggca
    40501 ggaggcaacg gtgaggagaa tcgaaggcgt tcgggggacc gtggtctgtg gattggtcat
    40561 tttcctggca gggtgcggga tgttccagcg ccggtcggac ggtccgtcct tccaggtgct
    40621 cgatgtgaac ggggacgggc aagtgaaccg ggaggaatat gtcaacgggg tcctgatgga
    40681 ttctttcagg aaggtggatg cggaccgcga ccaaaagatc acccgggagg agtgggccaa
    40741 ggtcaacaag gatcccgatg ccgcggcaca atttgaccga ctggacacca acagggacgg
    40801 ggtggtcact ctccaggaat ggattcgaga ggtggagaag cacaccaatg tgaccgaaac
    40861 ctttgatgaa aaggacgctc gccgggacaa tttcctcacc gtggacgagt actctcgggg
    40921 aagaaactcc gggttcacga tcttcaatat gaaattctga gctccccggt gggctgagct
    40981 ccgaccgagg ctccctcttc cctcaatcct tgaggatccg gtccaatctg ggtacggatt
    41041 ggggattgtc ccgggtcatg gttcggcttt cgcccgggcg agcggtgacg ttctcgtaga
    41101 ctccgtcatc ccttcgcacc agtttcgtga agcccaggtc ccggagttcg gtattggtct
    41161 ttggggtgtt gatgtttacg cgagagagga tcttccggac gtccctgccg cagttgggac
    41221 agcggctcag gggttcttcc cggatctcct gtcggatttc aaaggctttg ccccattcgc
    41281 agggctccgt gcagtgctcg tattcataga cgggcatggg agggatcctt ttttcgaggg
    41341 gctccgaagg aaattcctgg gggccccggc cgtctccggc gggaatttcc gccgacgggc
    41401 ttggtttcca gggggccgtc agagcaggcc cttgtcctcg agaacttttc tcacggtgat
    41461 ctccgtcctc tggaggtaca gcgccgttgt gccgctcagc acgtgaccca taagctcctg
    41521 aaccgtcgtg agggggaccc cggcccgaag catctcgacg gctcgcgtgt gccggagaat
    41581 gtgggggtga gacaggtccc ggggaaggtc ggcttcctgg gccctgcgat aaaattcccg
    41641 tcggaagttc ccctggtcca gggcaaagac ccttccccgc ataccgggga actcatcccg
    41701 gtaatccagg acctgccggg tgacaagggg cggaacggga acggtcctca agacgtttcg
    41761 gaggttccta tactccgacc gcaggatcct gacttcgttg cgatcgaagt caatgtccgc
    41821 aaggtcgttg aggcgaagca cctctccgat gcgggctccc gtaaagcgca aaaaaaggta
    41881 agacagccag tatcggccgc gcactctgcg gatgtagctg ctggacgccc ggcgatgcca
    41941 tagctcgaag gcttccgaga gcctccggag ctggtgttgg gaaaggtgtt cgaccgatgc
    42001 ggccgaggga ccggttgaac cgttgggcct ctccgacgtc ttgtccatgg cgtcactccc
    42061 tggtgccgtg accacgatcg tttgttcccg ttgctcgacg ggttctctgc tcgacgggtt
    42121 ctcttcatgt tttgattgta tcacaaagat ccagtttaca cgaaatacaa ttttctcgtg
    42181 tattgacagg gctggggacc aatcttactt tcaccctgaa aaaagcaagc cccacagcga
    42241 atggaagcaa atacccgtcg gagtgaatct cgaataagga ggttagctat ggccatcatt
    42301 cgatacccga gtctttggag tggcatgaat ccctttcgtg agctcgaacg tatgcgaaag
    42361 gaaatggacc gtctcttttc cgaagtcatg gaccggagca ctcatctcca atcttccggg
    42421 gtctacccgt cccttaatgt atcccaggac ggggaacagt tctttgtcca ggctgaactg
    42481 ccgggaatcc atccggaaga cttggagatt tccgtagaag gaaccactct gaccctgcgc
    42541 ggagaacgca agtccgaaaa cgtggaggga gtcagctatc accgtcgaga acgcccctcg
    42601 ggcaggtttc acaaggccct cactcttccc cacgaggtcg attcggacaa ggtcgaggcc
    42661 acctttaagg acggagtgct caagttggtg ctgcccaaag cggaacacgt caagccgaaa
    42721 aagatcacgg tgagagccga ctaagtcaaa cgagcaatca ctgcgtgacg aactcaaccc
    42781 aacaatagct ggaggagata tcatggagga caaggatttg caggtgcagg aaaaacaaga
    42841 gctcaagacc aagtccgaaa gcacccggaa tgttcccatc tacattccgc ccgtggacat
    42901 ctttgagtcc ccgtcggaac tggttttgat ggccgatatg ccgggggtgg ccttcgacaa
    42961 cgtggacatc gacctggaca acgatcaact caccatccgg ggttccgtca gggtcgagga
    43021 cgaaaaggga acggttttgt tgaaagagta ttccgtgggg gactactacc gccagttcac
    43081 tctctccaac gtcatcgacc gcaacaagat cgaggcgtcc atgaaagacg gagtgctcaa
    43141 ggtggtgttg cccaaggccg aggccgccaa gcctcgaaag attaccgtga agtccacgta
    43201 acgtaggtct ttcagtccac tcctatccgg accctcacga aaaagcagcg atttttcccg
    43261 tcaggagcgc cacgggcaag ctcggcttgc ccgtgctctc ttttttgaga tccggcagtg
    43321 aggaacgtct tttgcccgaa accctttggc caggcagccc ttgggctggg gaggcttctt
    43381 ccttgaaatt gcgaaaaacg gtctcgctta cggccttttt gtcctttatg ctcgtcatcc
    43441 tcacgagcgt catcctctac atcgttcccc agggaagggt ggcctattgg gcggattggc
    43501 gcctgtgggg cctcagcaaa acccagtgga ccgagatgca tctcaacctg ggcctgctgt
    43561 tcctgatcgc ccttggcctg catgtctact acaattggag cgccatcgtc tcctacctca
    43621 aagacaagac caggactctc gttctttttc cccgtgaatt ctcggcggcc ttggtcctgg
    43681 tcctggtctt cggggtgggg acctatcttg aggttccccc gtttctctgg gtccgcgaac
    43741 tgagcaccca tttcaaggag gaagcggccc gaaaatacgg cgaacccccc tatggccatg
    43801 cggagctgtc ttccctggag tcttataccg ggaggatggg actggacctg gatcagagtt
    43861 tggccgggct taagaaggca gggatggcct acgaccaccc gggtcagacg ctcttggaga
    43921 tcgccaggcg aaacggggtg ccccctaagg tcgtcgatca ggctttgcgg tccgcacatc
    43981 ccccggtgca tggaagcgaa gtccggaagc tccccgactc cccccccgca ggattcggcc
    44041 ggcgcaagat cgaggagatc gccggcgaat acggcctcga cgtctcccac ctcgtccgag
    44101 atttgactcg tgcgggaatc cgggccacct cccaggaaag catcaaggag attgcctccc
    44161 ggaacgggaa agcccccgtt gagatctacg aactcatccg tactcatcaa aacaaagggg
    44221 cccctgtatc accgtgaggg tattcctcgg aaacccggtt ccgccctcgc ccttatgtgg
    44281 ataaacccaa aggggagcat ctcgcggggt ttccattccc ttctgccggc cctttccgcc
    44341 cgggcaggga gattcgggga tcgtgatcct gtacggtctc tctacttgcg gatgaggggg
    44401 ttgcggatct cgtaggccac cagccattcg aagcccagtc ggccgggctt ggtgacggcg
    44461 agaaccgtgg ttcgaccggg ctgaatccgc ccgtaatccg aggaacggat gctgatggtt
    44521 tccggttggg taggggaacg ccacgattcc acctgaacat gataggtggt ggaattcttg
    44581 gacctggtca cgtatttttg gagcactctt gcctcgtggc cggaatgcgg agaggtgtcc
    44641 aggatgccgt tggccagggt ttcggcaccc cctccggcga cgagaaagcc cgccagagac
    44701 aaaaccagca ccaccaggag ttccttgtga gaggtggacc tgccgaccag ggcccggacg
    44761 gcccaccaga gaaagatccc cagtccaggc aggctgtatt tcagggaatc caggaacacg
    44821 tcctcgggat ccagcggggt gaactcggcc ccgccccaga tccaggacgc ggttccggcg
    44881 accaggagca gggaggggat ggcgaaggcg gcaattcttc gggtcttcca ggaggggtcc
    44941 tcgatatacg ccggggggac gtaggacacc gaggggtcct gggccagccg gatgagcagc
    45001 tgcgccgccg cgggcaaaaa gctcgggtct atgtcctccc ggaggggaaa gggggtcagg
    45061 acggcttcca tgcccttctt gtccagcttg atttcggtga aaccgagcct gaagacgtcc
    45121 cggaccgcct gcctgcgctg ggcgtcggag aagaatcctc gggcgaattc ctcgtcgctg
    45181 ctcgagatat agaattcccg gtcaaaatcc gggtcacccg tctggatttc ccgggcgatt
    45241 cccaggttct tggaaaagcg atcgaaagcc ttctccctgc cgatcttgaa gcttccttcc
    45301 aagggactgt cggtagccac ctggaagcgg gacggcgagt ttccgctgcc gggaaaatac
    45361 tcgtaccgat aagggacccc ctcgtggacg gcggtgaact tccttccgga ccccacggct
    45421 cccacttttt gcgccaggag gtccagcact cttcgccgct tccgggaggc aacgaaaagg
    45481 aagaccagaa gaacgaccag gaagagggta acggcggacc cgacaaggat gatcggcagc
    45541 atggaaaggt cctttaggca ggggggaaaa tcctcgattt cacccggttg cggatccaat
    45601 gtgtcccggg ctcaacggtc cggcattcca taagatcttt gactgaagag gccacctatc
    45661 gtagaacatg gaggaaagac acgcaacccc catttttgga tccggggagc ctccggggag
    45721 gacgacgaaa atggagagaa tccggaacta cagggaactc ctcgatgctc tcttcgacgg
    45781 ggtttatttc ctcgaccccg agaggaagat cgtctactgg aacagtgcag cggaaaggat
    45841 cacgggattt tcggcggcgg aagtcgtggg gaaatcctgt gccgatggaa ttctccggca
    45901 tgtggacgag aatgggacgg acctgtgcaa gggcttgtgc ccggcgggca aaacccttcg
    45961 ggacggcctc atgcgggaag cggacgtctt tcttcaccac aaagaggggc accgggtgcc
    46021 cgtgagtgtt cgggtgactc ccttccggaa caggaaagga gaaatcgccg gagccatcga
    46081 gcttttcagc gacgggagct ccaagaacgc catgatcgag aagatccggg aactggaagg
    46141 cctggcccta ctcgatcccc tcaccctgtc cgggaaccgg cggtttctcg agatgagcct
    46201 ccaggggcgc ctccaggaga aggcccgaac cggcatttgt ttcggagtgc tgtttgcgga
    46261 cgtggatcac ttcaagcggg tcaacgacac atacggccac caagtgggag accaggtcct
    46321 gagaatggtg tgcagcgccc ttcgatccag cttgagaccc tttgacgact tggggcggtg
    46381 gggaggggag gagtttgtgg tcctcttgcg aaacgtggat cagcgggggc tggaaagggt
    46441 ggccgagcgg cttcggatca tggtgaagaa atcgtttcta tccctggacg ggcttgaaat
    46501 cggcgtcacc gtgtccatgg gagggaccct ggcccgggac gaagacacgc ccaccagtct
    46561 tctccagagg gcggacgagc acatgtaccg gagcaagatc gcgggcaggg attgcacgca
    46621 cacggacgaa ggccgcatcc ctcccctttc ggaccctaaa ggcccggcat agaaacctcg
    46681 gcgggacacc gatgaaccgg acggccaccc gcctttgccc tcgaggcgtc ccatgaacga
    46741 tcccgaaaag aagacctgga gggaacgcct ttcggaaggc tctgcccgcg gaaggaagaa
    46801 atttcagagc ctccggcggg ccgtgggaga gatttttcga ggaatgaccg tccatgaact
    46861 cgaaatggag ttgaggaagg aaaaggcgac cttcgatcag ctgttcctgc tcatcgtttt
    46921 tggagacctc atcgggctcc ccctctttcc tccctactac accatgcggc tcctccccta
    46981 catcctgccc gggatcgaga gctggaaaag gagcgtcctc cgggaaaaag acctctcgga
    47041 cctcgtatcc ctggacatgt gacaccgggc ggcccccggt cgggcggctc acccggtagc
    47101 gtgagcggca attcttcgcc gcagggaccg gactctttcc gcaatgtccg gggcctgggt
    47161 gatggcggtc accatggcca cccgccgggc cccggcggag agcacctgat cgaggttgga
    47221 ttcgttgatg cctcccatga ccgtaaaggg cacccggacc cgggcaccga tctccgtgac
    47281 ggcccggggt ccgaggaatt tcttgattcc ctccttggtc ccggtgggga aaatgggacc
    47341 gacgttgatg tagtccgccc cgttttcctg ggcctcgagg gcttcctcga tggagtgggt
    47401 cgaagccccc aggagcatct ccggtgccag gcgccgggca acggccaggg gcaggtcctc
    47461 gagacccagg tgcacgccgt cggcctccac ggccagggcg atatcgaggc gatcgttgat
    47521 caacaggagc accccggcgg cggccgtcct ttcccggaac tcgagggcca tgcggtaaaa
    47581 ctccagggta gaaagctcct tttccctcag ctgaacgatc ttcgccccgc cccggaggac
    47641 ctcttcgagt acttcccggt tggtacgccc cgccgagagc cgctggcagg tcacggggta
    47701 tacatccacc tcctgaaaga gggcctttct ttcgtcccga ttcatctatc cgcctcccac
    47761 caaccgtacg atctcgatgg aatctccctc ggcaacgggc tccgattcca tggcgtcccg
    47821 gggaacaatg ttcaggttcc gttcgaccac caccgttttg ggattgattc ccaggagttc
    47881 gaggagcccg attatggtgg tgggtgactc caggtgccgg ggctttccgt tgaccgtaat
    47941 ttccatggcg ttctccgtaa atgggtcctc cccgggggac ttgccggtta ctggaggaag
    48001 gcggcgtccc aatccttcca gacgggttcg tacccgcggt tgcggatgac ctccgccacc
    48061 gcccgggggg ggcggtcgtc gcagacgctg aattgggctt ccgcgtccgt gggacggctg
    48121 taacccccgg gttccgtgtg agagcccgcg ctcatggtgg tgacgcccag ggggatcagg
    48181 tgatctcgaa aggaaggagg ttcccgggtg gaaaggacca ggcccgcatc cggcaggaag
    48241 agccgaaggg ccaggaggag ctggacaaaa tggaggtcgg agaccgggtg gggaggttca
    48301 aatcgaccgg ccgccggtct cagtcgagga aaggagatgt tgacctggga tcgccagaag
    48361 tttcggaaca ggtattgggc gtgaagggcc aggtagaacc cctcgacccg ccaatcgcaa
    48421 aggcccagga gtgcccccag tcccactcgg cgaaacccgg ctcgagcccc ccgttcgggg
    48481 gtttccaggc gccacagaaa gtcccgtttc tttccctggg gatgaaaatc cccgtagcgc
    48541 tctcggttgt aagtttcctg gaacaccacg aggccgtcca cccctgcgtc gatgagggcc
    48601 tcgtaggcgt cgacccccat gggatgcagt tcgatggaga tggaggaaaa ccggggtcgg
    48661 agccgatcca gcacctgcct caaatagtcg agggtcaccc ggtgggggtt ttccccggag
    48721 acgagaagga ggtgacgaaa ccccaggtcg tgaatggccc ggccttcggc ttccgcctcg
    48781 tcgacggaaa gagtccttcg ttcgatggga ttgtggcggt tgaagccgca gtaaacgcag
    48841 gcattgacgc actcgttgga gaggtagagg ggggcgaaca tctggatggt gcggccgaac
    48901 cgctggagag taagccggtg ggccctgcgg gccatctctt cgaggtaggg cgtcgccgcc
    48961 ggggaaagga ggctgagcat tccctcgagg ttcacggacc gcccggccag ggcggcctcc
    49021 acgtccgagg cgctgcggcc ccggacctgt gcggcgatct ccttccacgg atgttgactc
    49081 atttggtcat ggaaactcat ggcgctcatc cttgctcgag tctcaggaac cctgtgagcg
    49141 ggctcgatgc ttcggcggtc tttctcggtt tggccgggcc tgccaggaag gcctggcgac
    49201 ccgcttcggt tccttttcga aaggcccggg ccatggccac cgggtctccg gccacggcga
    49261 tggccgtgtt taccaggacg gcgtcggctc ccagttccat ggcttcggcg gcgtgggacg
    49321 gagctcccag gcccgcgtcc accaccacgg ggattcgagc cttttcgatg atgatctcga
    49381 tggagtcccg ggttttcagc cctcgattgg aaccgatggg ggaccccaga ggcatcaccg
    49441 tggccgtgcc gacctcctcg agtcgtttgg ccaggatggg atcggcgggg atgtagggaa
    49501 gcaccacgaa cccttccttg accaggatgt gcgcggcctt gagggtctcg acggggtcgg
    49561 gaagcaggta ctggggatcc ggggtcactt cgagtttcac ccacggctcg cacccggccg
    49621 ccctggccag ccgggccagc ctcaccgctt cgtcggcgtc cctggccccg gaagtgttgg
    49681 gaagcagcag agtacgaccg gtgtcgatgg cgttcaggat gctgtccccg gggtcctcga
    49741 gatccactct tcgaagggcc acggtgacaa tctcggctcc ggaagcctcc agtgcctcct
    49801 tcatgatctg cggggaggag aacttccccg ttcccatgag gaggcgggag tggaacacgc
    49861 gacctgcaat gatcaaagga tcgttcattt ccgattttcc tctgctaaga atagtttcca
    49921 caaacacggc atcgcttctc ctgcgggagc ctcacttccc ggaagatccc ggccagggcg
    49981 tcccaggtga ggagtcgtcc caccagcagg tctccggccc ccaaaagata ctttacggcc
    50041 tcggtggcct ggatcaggcc caggaccccg ggaacggttc ccagcactcc ggccttggag
    50101 gtggtttcca cggcgccttc ggggggaggc tcctggaaca ggcatcgaaa gcacgggccc
    50161 tttcccggca ccacggtcat ggtctgcccg taccatccca ccgctcccgc atgggaaaag
    50221 ggcttgttca ggcgcataca ggcatcgttg atgagaaact tggactcgaa attgtccgtg
    50281 gcctcgagga caaaatcgta gggagcaagg atctcctcgg cattgtcggg ggtcagccgg
    50341 gacgaatgga ggttgagccg gacttcgggc ctcacccggc gcagggtttc cccggcggac
    50401 cgggtctttt ccttgcccag gtcttcctcc ccgtggacga cctggcgctg caggttcgac
    50461 agatccacgc gatccccgtc cgcgatcccg atctccccca caccgcaggc acccagatag
    50521 aggagggccg aactgcccaa cccccccgct cccaccacca gcacccggct gcccaggagc
    50581 ttgatctgac cctgctcccc gaagcggtcc aggagcacgt gacatcggaa gcgttcccgg
    50641 tgacgggtag tgaggcgagt gggttctccc ggcataaaaa agaccgtccc ccaactggga
    50701 aacggtcttg gaaggaatcg gaaaggatgc ttcgctttca accgtttccc tacgctggca
    50761 ttgtccagat caggtacaaa gggtgttttc tcagcccaga tatctgagca cccccaacgg
    50821 ctgt
//
LOCUS       DAOOVC010000012        49292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_450_length_49292_cov_6.145643, whole genome shotgun sequence.
ACCESSION   DAOOVC010000012 DAOOVC010000000
VERSION     DAOOVC010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 49292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49292
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_49292_cov_6.145643"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLM79_03025"
     CDS             <1..320
                     /locus_tag="PLM79_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85305.1"
                     /translation="HGHIARLDYRQRKDVQVDEIIPQLYVNVLPNIFVTTYHDYSMDK
                     GELFSQGYGVLYQKGCWGLGLMYEKEAKDQRIAFTINLLGLGSIGGSRTYRGGGNLPS
                     GFP"
     gene            859..3561
                     /locus_tag="PLM79_03030"
     CDS             859..3561
                     /locus_tag="PLM79_03030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOV85306.1"
                     /translation="MTRTDMAEIRETLFQRVFDSLGDPVVVYDRDLRILDVNVAMTER
                     FPAAREEILGKPCGELLYGRGNLCEPCGVRQAFHTGRPFVGEKTVSLPDGNTRVWELR
                     IFPVKGSRGKIREVAEVVRDITEQRGLEKELRDSEERYRTMVEIAREGIYIVDAETRV
                     TFANQCLARMLGYPMEQVLGRSLLEFMDEESRGIARQQFERRRRGLSDVYELRLTKAG
                     GAPMNCLISVAPLVDNGSFRGAVGLITDITCLKRTEAKLRAAKAFSERIINSITDSLV
                     VIDPRSHRIVQANESFLARVALGSLSELSDPTCFRVLLGREEPCDPEKTGCLVRDTFE
                     SKRPARGDRSYVDARGTPRVFEVTTYPVMNSQGEVDLVIRLDHDVTERRKMEEALANR
                     SLELQKAQQQLEALIDISRRVSTKGSISETLQYLHGVGRKIFPQSDSLFFILNPAGDQ
                     FLRLEECPAALSGPLHQMMEQISHGGLLADFCDFLAKNKQQQIVHHESDNEKHPVFRI
                     FSQGYPSWFGVFLCPKNQCLGLYVLGSRSSREYPQEDLRFFQALFEQVAGHIRHLVQH
                     ETEVHQLKEQAAERASYGEIIGRSKKMQEVYELIDLVSSSDATVLITGENGTGKELVA
                     RAIHKQGHRSKGPFVVANCSAYSPTLLESELFGHERGAFTGAIHQKKGRIERAHGGTL
                     FLDEIGDIAPATQILLLRFLQDHCFERVGGEKTIEADVRVLAATNRDLLQEARGGRFR
                     DDLYYRLNVISIHLPPLRERKEDVPILAEHFLRKSTLKEGKKIHRFSSDAMQALMDHE
                     WPGNVRQLENAVSHSVIVAREDVIKRKHLPRFLREGPEGPSSTSLAENERQLVLRVLR
                     ESDWNKHEAARRLAVSRSTLYSMIRRYRLEPAPGGSREISGVDS"
     gene            4365..5852
                     /locus_tag="PLM79_03035"
     CDS             4365..5852
                     /locus_tag="PLM79_03035"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOV85307.1"
                     /translation="MAEKKEVEINSRADTLPLMSPEVRPWPVTPAPSPEEVEAIYARR
                     KATEFGKWLEDNLRLEKSWGKPEALQGMKVLCMGMWRIGNKFAASQLAESGAEVINIE
                     PPGGDPLRKLTPFGREEYMLADRDTGEKCGLDFISEMRNMQSITLNIETEEGREVYRK
                     LATHADVLIEEYPPGYMDGLGVGYRQLSKINPRLVYCQMAVRGTWGPYKDKLSKHGQW
                     TLEPFGQCANSFVHNTGFPQDQLPRGRGGDPTRSGVWFADYVAGEQAAVNILAALYYR
                     EEVSGKGQLIEVTGAEAQMDILDFDIGWYGFNQSIKARTGGWDPNLNQYEWNPCKDGY
                     MMIGGQTDRLWYRIGMCIERDFPQFGRLIHEDPTLKEMGARNALQALIKTYTLTTRWL
                     RDTNRIEAEQKLMEYEIAAGPVLFIDEVCEFPHFVYRPWVYNLDSEQYGTVLYAVPPN
                     AYQQRTPARVKWLGRPLGKDTYEVCMKYLGMGQTKINEYKDKGVM"
     gene            5852..7267
                     /locus_tag="PLM79_03040"
     CDS             5852..7267
                     /locus_tag="PLM79_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOV85308.1"
                     /translation="MAKDLREVRLEDLPGPSSKEELEEMKMPYEDYCRKMFDVGGEFG
                     KPQALKGIRWLSTTMYIFTPHSVANLAELGAEVIKIEMPRMGDPMRHTSPFNEAYLYP
                     LHDSRPMTGTGFGFLGANDSEYHITMDYHLDEMKEAFYRLVKMSDGLTECYRHGTFDR
                     WKQSYRYLQELNPRFIYVWGGGFGYGPKIFGGSYDILGQAHAGLASVTGMHDFMGGHV
                     AKHSNWCIDWYSGTLITYGILAAMHWRRKTGLGTMIEFSQVQAATRALGYTVPLYGRF
                     GIVRQRWGNWDTQLCVHGIILCGKSDFPDSENPQDKYDARYVMVSAFQDADFKELCDI
                     TGLKELYAKYQAHKDRVEAEPQVEIYAALEKWASDKSRSQVVKILQDAGILAEPVMND
                     REVYECEHYRQRGTIRWHEDPIFGDVLLQSTYSTGLMSETPRRQSWIWRPVGADNVKI
                     YHELMGYPIKKVEDWYAKSWI"
     gene            7282..7767
                     /locus_tag="PLM79_03045"
     CDS             7282..7767
                     /locus_tag="PLM79_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85309.1"
                     /translation="MCDIIWCKKEIDGKVCNTVNYLDPYCFWNWEGKVNCAECGTVYY
                     IHMIQGFMYKGPEEKPGEKPDTSPLVADQPLNGYKCHIPGIEGRTRPYQCLPRGIYLG
                     RADMVKFSKRGRLVRGWRPQPPSSGIAGSFGFNWDAKKLSPDVWEEYQEKLRKGEAKD
                     W"
     gene            7786..8013
                     /locus_tag="PLM79_03050"
     CDS             7786..8013
                     /locus_tag="PLM79_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85310.1"
                     /translation="MAQLTAQEFLDTVLVPEKQSDHICWQCRFLRPVLKNSKFPPADI
                     IGWCTKLHWPHYWCVADFNVVKKCYAFQSKR"
     gene            8019..8276
                     /locus_tag="PLM79_03055"
     CDS             8019..8276
                     /locus_tag="PLM79_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85311.1"
                     /translation="MEEQISRELEAIKKMVQTWKGSYLREAPENGKAEYLVEDLSQEI
                     RTFVHPYVRRFLELGYLSESEAREFLDGCDGEVEDLRRIVG"
     gene            8385..8807
                     /locus_tag="PLM79_03060"
     CDS             8385..8807
                     /locus_tag="PLM79_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85312.1"
                     /translation="MPAKCRVVVCGFDPMLVRGYVKTGYRALWWHMPDEIWEEHRPLR
                     GKTVSGKVLAVYNGDGAKTASPNEHYEWNTAKESGLAVVIPPEAIVKYQLTEFHFLEL
                     TIEHVGGRDVYPGEEKMSSKWWPEEKMKLDYKLAYVAP"
     gene            8992..10215
                     /locus_tag="PLM79_03065"
     CDS             8992..10215
                     /locus_tag="PLM79_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85313.1"
                     /translation="MITEQGRDAGKESQDFQTDPQHPGWLPLDSILKTGEPIFQRFLF
                     MLGFDISSNIYLSTGESLAVVDPGNDYTAFMELFGKGYAPEAVKKVVLTHGHRDHSMG
                     VFELLRAYPGIRKSGGFELVLHEAAPPELKQVATEFGCRVTEVRGGETVRLGDAEWEV
                     VHTPGHTIDGICLVHAASKTAFTGDMVQPHLMAEPDKNAGGDLNHYLYGVKELLRREI
                     ENILPGHGAPIASLGRRIVEETYVGLLMRIIGAEAPIPWMDGAMALLKKGLPEETVFC
                     LEKELAQRPMNPRALHVKALCLNDLGRFEEALHTLDELQGLEAEEPEPFSILARGYAL
                     MGLGRYDAGIAFFDRVLQRDPRMQDALVYKGMALYLSGRREEAMEIAPFRNEFVGRFK
                     EAISRTAGVHGPGPG"
     gene            10336..10767
                     /locus_tag="PLM79_03070"
     CDS             10336..10767
                     /locus_tag="PLM79_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85314.1"
                     /translation="MNSESDHPLVLLLVCNKETREYLSDLLRRNSYSPQAFSVPADLL
                     NHLRAGTDAIVFVDGQAVSNCGAGIFSKIRESCSGCKVIFLCDRAHRELIKEAMGLGA
                     YGCVLEPYEEWEVLTMVRHVLSDLKPKRRRSSKKTRRPAEG"
     gene            11080..11802
                     /locus_tag="PLM79_03075"
     CDS             11080..11802
                     /locus_tag="PLM79_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85315.1"
                     /translation="MSCFFAITTRDRIYLVADNRRRIFGRAGERAFDGAVLLDDARKI
                     CRVCDSFWVAGTGLHEFLVRMVEEIRSAWIPREGREGPDLKDLLTLRTWEETCRKIHG
                     EAVTECRRAWERTKAGVWDPGESRGEILLAFLPQDRHPVLLHGKSGEDFVLSRHYGPG
                     RVIVSSLSSSGDGFPDRELSARLEWVARELLKSDPSDEIRRAYDLLPPLLGFVSEIMP
                     REVSACGDLVVIHPGGYEWWLF"
     gene            11913..12134
                     /locus_tag="PLM79_03080"
     CDS             11913..12134
                     /locus_tag="PLM79_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85316.1"
                     /translation="MTWTAVVFWDTQDPADVGWRWKIQDLEGGTCLSGAAASAEEGIR
                     RVKALLKEKGIDPGSVWMDILDDGVWDKC"
     gene            12245..12679
                     /gene="rplM"
                     /locus_tag="PLM79_03085"
     CDS             12245..12679
                     /gene="rplM"
                     /locus_tag="PLM79_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOV85317.1"
                     /translation="MKTMSAKFEATGRKWILVDAEAQALGRLASQVAVRLRGKHLPHF
                     TPHVDVGDFVVVINAEKIRLTGNKWDQKVYYRHSGHMGGLKATTAKKLNRENPERMIR
                     LAVWGMLPKNRLGRQLLKKLKVYRGSEHPHEAQQPTQVVLPT"
     gene            12705..13097
                     /gene="rpsI"
                     /locus_tag="PLM79_03090"
     CDS             12705..13097
                     /gene="rpsI"
                     /locus_tag="PLM79_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOV85318.1"
                     /translation="MPEQRFYATGKRKTAVARVWLQAGTGRVMINKKPVDDYLDRETS
                     KMIISQPLILTGTYGKMDVLVSVNGGGGSGQAGAIRHGISRALLEFNPEFRDILKKAG
                     FLTRDSRVKERKKYGRRSARARFQYSKR"
     gene            13371..14408
                     /gene="argC"
                     /locus_tag="PLM79_03095"
     CDS             13371..14408
                     /gene="argC"
                     /locus_tag="PLM79_03095"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOV85319.1"
                     /translation="MLRVAVAGASGYTGFELVRLLCTHPRAKLTMITSRAQKGQRIDE
                     FYPALRAHCDLVFQDPDPVELAARAELVYTALPHQAAMDLVPQLLDRGARVIDLSADY
                     RFIDPAVYESWYQPHKTPELLSEAVYGLPELHREAIRNARLVGNPGCYPTSVILAMAP
                     LLKESLVEPGTIIADSKSGVSGAGRGVSLGVHFCEVQEGFKAYKVAEHRHRPEMEQEL
                     SRIAGKPVRITFTPHLVPMTRGILSTVYASLKDGVDLSRVEQAFRSFYQGAPFVRLCG
                     SKDLPTTLQVRGSNYCDLGWRVDEPTRRIIVVSVIDNLTRGASGQAVCNMNLMFGFPE
                     NLGLDFPPWQP"
     gene            14479..15228
                     /gene="truA"
                     /locus_tag="PLM79_03100"
     CDS             14479..15228
                     /gene="truA"
                     /locus_tag="PLM79_03100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOV85320.1"
                     /translation="MPRRNLRCVLEYDGSQYHGWQRQRGVLTIQEVLETRLEIMLNRP
                     MSVRASGRTDAGVHARGQVVNFYTETRMGPEEVQKGLNSLLPGDVVVLRVEEVSDSFH
                     ARFSAKSKTYEYRILNRPTPSALERRYAWHIRSPLYLDPVLESLELLTGTHDFAAFMA
                     SGSSARSTERDLMRASVSRPDPEHVVFRFEANGFLRHMVRNLVGTLVEVGLGKRSSQE
                     IPTILTGKDRREAGITAPAHGLYLVSVQYDE"
     gene            15259..16458
                     /locus_tag="PLM79_03105"
     CDS             15259..16458
                     /locus_tag="PLM79_03105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOV85321.1"
                     /translation="MKLSQRVARIKPSATLSINAKAKALAKQGIRIINFGVGEPDFDT
                     PEYIREAAVDAIRRGHTRYTAVGGIDELKDAVLEMIRNEYGLTYGRENVLVSCGGKHA
                     LYNLFQALLDPGDEVIIPSPFWVSYPDMVELADGKPVFVECPESLGFKLRAQDLEKAI
                     TPRTRLLIWNSPSNPTGVHYRRSDMEPIAQVLLKHEHVAVVSDDIYCRILFGDHEWCN
                     LPMVEGRLKDRTFIVNGVSKTYCMTGWRIGYLVGDRDAVRAATDIQSQSTSNPTSIAQ
                     CASAAALRDHESEIRSMVREFESRSRYVLKRLLEIPGVSCPQPDGAFYVFPNFGHYLG
                     RKYGGKVIRDSGELAQYLLEQAHLAVVPGGAFGEDRCLRLSYALSMKDLEEGFNRLEK
                     ALAALEK"
     gene            complement(16694..17350)
                     /locus_tag="PLM79_03110"
     CDS             complement(16694..17350)
                     /locus_tag="PLM79_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV85322.1"
                     /translation="MGDTKKIVIAEDHTILREGLRLLLTSCPEYGVVGEAKDGLEAVR
                     LCSSLKPDLVLMDLSMPRMNGMGAIAEIKKACPDTKILVLTVHKTEEYILATLKAGAD
                     GYLLKDATHSELMLAVDCVLSGKSYLSPGVSEKVIEGYLEGKKRVKTSTSWDTLTQRE
                     KEILKMVAEGYKNKDIADYLCISAKTVEKHRANLMKKLDLHSASALTSFAMDKGLIGN
                     "
     gene            17723..18109
                     /locus_tag="PLM79_03115"
     CDS             17723..18109
                     /locus_tag="PLM79_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV85323.1"
                     /translation="MHSTLIVEDNPIFRSSLRDILLSHFPRMQVLEAENGSEALEKLR
                     KDIPDVVFMDVRLPGQNGLELTRKIKAAYGDVAVIILTSYDLPEYREAAFQCGADHFC
                     VKGSTTGEEIVGIVGSLLVERGEKLP"
     gene            18446..18700
                     /locus_tag="PLM79_03120"
     CDS             18446..18700
                     /locus_tag="PLM79_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV85324.1"
                     /translation="MSNGIVLDYLSADEVKMKMSMTGGILQAQKWLVVYNALVDPRPV
                     REIAMHTGLSEASVVRILSEYNQHGPTALSPTGSSGKRPS"
     gene            18774..20705
                     /locus_tag="PLM79_03125"
     CDS             18774..20705
                     /locus_tag="PLM79_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOV85325.1"
                     /translation="MSRWGFLLALITLGLLFASDSGAYPAGIHPDLESILAGKGDRDE
                     LSVIVTLAQTVDLDPSGAVPRKALRGAMIRALKRFAASSQAPLKAFLSREGVVAVESL
                     WLINGLAVKARPDVIRKLAQLPGVSQVLPDRIIPLFDTSPVIGPMAQEWNLSAVEAPR
                     VWRLGFRGQGVVVANMDTGADGNHPDLKSRWRGGANSWWDPFRKTSAPYDVQGHGTQT
                     MGILVGGKAGGTAIGVAPKARWIAAKIFNDSGSSTSSVIHQAFQWLLDPDGDPDTDDA
                     PDVVNCSWGVVNSPGRCIEDFHRDIRVLKRAGIAVVFSAGNSGEVGPDSSISPANNPG
                     VFSVGAVDSGNNVAYFSSLGPSACTGKFFPTVVAPGTGIKSADPVSAGVQGSYAAVDG
                     TSFSAPHVAGTMALLLSAFPGLSVPTLETALKASALDLGNAGADNLYGRGLINAFEAL
                     RYLQSRVTQVTLNSVNAQDGWIRESSKVPGTGAAARSAGNVLVGDDAGNRQLVGFLSF
                     DTSILPADAVITSAKIFLTRKAVRGTNPFALLKKCFVDLADGTFNTAALEIQDFQAAT
                     PFPQVARMSNPAQNGDVSVGELGETALRAIPKGSVAQVRVRFAKTTNGNSVADQVVFW
                     GDDGVGSQRPRLEITYMLP"
     gene            complement(20702..21640)
                     /locus_tag="PLM79_03130"
     CDS             complement(20702..21640)
                     /locus_tag="PLM79_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV85326.1"
                     /translation="MGERSPVPGADSFSREADYAKRCVFLLLALILWFPCPAAAEKWS
                     FVALGDNRHGFAAYLDLLEGVAQLHSPENPRQPPLDFALICGDFTPVSESYESFRRLL
                     GPPPPFLLPVRGNHENREDVEVILGSILPSLGPRVRSPHEKSVTYFADWKNVRLVVLD
                     PLSDFGSVFGGDRVIKWLPAVLEPPSSIQHVFLAMHKPLFLNPRSHWKLWQVLLQHRE
                     RVRGVFTAHTHAYDHKRFPHEDYGIDVINVGNTGSVSHGDRRQTIVRVLVEGPDVAFE
                     IYQSTAVRKKFQWWEGWSSRATTTKEAARSGKEPRP"
     gene            complement(21640..24570)
                     /locus_tag="PLM79_03135"
     CDS             complement(21640..24570)
                     /locus_tag="PLM79_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOV85327.1"
                     /translation="MKSIHGFRLEREAEIPEINTTASFYRHEKTGAELLSLSNEDENK
                     VFGITFRTPPGDSTGVAHILEHAVLCGSRKYPVKEPFVELLKGSLKTFLNAFTYPDKT
                     CYPVASQNVQDFYNLIDVYLDAVFYPRISLPIFQQEGWHYELEGPNAPLSFKGVVFNE
                     MKGAYSSPDNLLAERTQQSLFPSTPYGLDSGGDPAQIPSLTYEQFRRFHRKHYHPSNA
                     RIFFYGDDDPDRRLRILDEYLRDFEQAEVDSRVDLQPRFSSPRRVEHLYAAGSWGDGE
                     ESGTMKGMVTVNWLLAETADPELNLTFQVLHYALLGMPGSPLRKSLIESGLGDDLAGV
                     GLETELRQMYFSTGLKGVPTADLDRVEPLVLETLERLCRETIHSDTIEAGINTIEFRL
                     RENNTGAFPRGLSWMLRSLTTWLYGEDPLKLLAFEAPLTSLKSRIASTPRFFEEMIRL
                     HFLENPHRTTVVLRPDPDLLRRMEAEEQERLAAFRRSAAPEQILEVIRATAELRRLQE
                     TPDPPEALSTIPSLRLSDLDKKNKRIPCESAEKNGLPLLLHDLFTSGIVYLDLGFNLK
                     LLPRESVPLVPLFARALLEMGTDREDYVSLTQRISRKTGGIRPELFSSIRRREGKSAT
                     WLFLRGKAMSGQLRDLFEILGDVLSRPKLDDRERFRQMVLEEKARQEHRMVPNGHQMV
                     NLRLRSRFNEADWASEQMNGVSYLLFLRDLARRMDTDWPGILAQLESLRQVLVRRDSM
                     VLNVTTDQAGWSVTDPLVGEFASRLPTGSTPETVWQPDPPEGHEGMIVPSQVNYVGKG
                     LNVYADGIPFHGSFSVVTGYLRTSWLWERVRVQGGAYGAFCMFDRHTGSLTLVSYRDP
                     NLSKTLDSYDQAADFLKKLDLSESELTKAIIGAIGTLDAHMLPDAKGFTSLLRTLIQD
                     DEQERQVMRDQILATRASDFRAFAEVIEGVKARGTVGIMGPQAAMAEAARSLGDSLRV
                     WKLL"
     gene            24939..25721
                     /locus_tag="PLM79_03140"
     CDS             24939..25721
                     /locus_tag="PLM79_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOV85328.1"
                     /translation="MKITFLGVGEAFDEELPNTSVLVRTTARGEPARILLDCGFTVPS
                     PFWRKAPGPQDLDLVWISHFHGDHFFGLPALLLRFWEIGRTRPLVLLGQTGLGDVVRK
                     TMELAYPRFLQKLAYPLQFVEVEPGRIFAWKDLEFSFAENDHGLRDLAVRLDLGGRSV
                     FYSGDGRATEQTLSLAHRCDLVIHESFRWKDPTPGHGTVAESIEFARRAQATRLALVH
                     LQRDERREKAHRIREVLQGLEGLEGILPQAGDEIVLGESPPR"
     gene            complement(25767..26546)
                     /locus_tag="PLM79_03145"
     CDS             complement(25767..26546)
                     /locus_tag="PLM79_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOV85329.1"
                     /translation="MPDQPEQSPEVLPLPEEQKTKNGTRNAVWLRLGILLGILGIIYL
                     LHESGIFKFFMHKDRLLAFLDSLGPLSFLGFIALQSIQVVIAPIPGEVTGIIGGYLYG
                     PTLGVILSTIGLVIGSFVAFSLSRAFGRPFVDKFVSKPVLERFDYLLHRKGLVVIFLL
                     FLIPGFPKDYLCFILGLGHLTSMEFLLVSSVGRLFGTILLTLSGGFIRNHQYGRLYIL
                     IAIAGILLGAAVLFRKKAEHLLKVWHLGDYKKSVKRTNSNG"
     gene            complement(26923..28296)
                     /locus_tag="PLM79_03150"
     CDS             complement(26923..28296)
                     /locus_tag="PLM79_03150"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOV85330.1"
                     /translation="MMIPQEILEKIDKNRQRYVEELLGFLRIPSVSTYTRHAQDVRKA
                     AEWVLHHLKRLGLPAQLHETGGHPVVTARSPFQPDLPTLLIYGHYDVQPPEPMEEWIT
                     PPFSPSIRDGCVYARGATDDKGQFFTYLKAIEAVLAVTSRLPINVKVLVEGEEEIGSP
                     NLEGFLKRHRTDLKADAVAVSDGSQFSRGVPAITYGLRGLSYFQIDLQGPRIDVHSGS
                     FGGVTANPAQALAQVLAQMKNPDGTVAIPGFYESVVPLEAWERQELAALSFDEGGLKA
                     YLGVDELPGEPGYTVLERKSARPTLDVNGIWGGFQGEGAKTIIPAKAGAKVSMRLVPN
                     QSPSVISALFKQYVESLTPPGVRVTVTELHRAEPVLVSRDCTGIRAAARAIEIGFGKT
                     PVFIREGGSIPIVNVLKEVLEIDSILLMGWGSPDDGPHSPNERLFLEDFHRGVRSAAA
                     LLYALAP"
     gene            28758..29879
                     /locus_tag="PLM79_03155"
     CDS             28758..29879
                     /locus_tag="PLM79_03155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV85331.1"
                     /translation="MVFESFSRAEQVDKELWNRLAQHAAPTMEWEYFRALEESGAVSR
                     DKGYRPFHLVGYEGGEPVLLAPFYERDRAWVEFGDGGLIEFLTELTGMPFNVGLVGTI
                     PFTPVPGYRFLHRSGEEPVALYRALLHYLDHLCETKGFATCRIYFTAADAVRLREVLK
                     GHGYVGLKNEHCRWFNPGFTSFEEYLGTFRSARRTKIKRELRTVRDRGVAVRMLAGED
                     VPASYFGRMYQLYRRTWEKHMGTLIAPFLNEDFFRLLDENFRHRCAFSTAFREEEVVA
                     MAIFYAKERKLYGRYWGTFEEIPFLHFATCYYHPLDYAIRQGMEMMDPGFGGEHKLFR
                     GYEIVPVYHFIKFYGEEPRRVAGAVLKQMSDRGRPVGFR"
     gene            complement(30200..30532)
                     /locus_tag="PLM79_03160"
     CDS             complement(30200..30532)
                     /locus_tag="PLM79_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85332.1"
                     /translation="MKSAFRLALAATLLLSFCALARADELEVRSKGGGAWDICYQGRD
                     CVGTLQPYQDNGFNLYKSSGDFVGTIFKDGRMQPPGRWVMLTAEDATLYLEALKAIAL
                     IKDKKKGP"
     gene            30789..30995
                     /locus_tag="PLM79_03165"
     CDS             30789..30995
                     /locus_tag="PLM79_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85333.1"
                     /translation="MTTQLTDGQKYLLPNGEVVIARHLADGILLEFKRKYRVPLEVGE
                     TGALILRGNETAWRVEQLTPVKDD"
     gene            complement(31118..33025)
                     /locus_tag="PLM79_03170"
     CDS             complement(31118..33025)
                     /locus_tag="PLM79_03170"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOV85334.1"
                     /translation="MVDLTRNRYFDLLQGKMSAESFHKLAVLNNEKLFNFVGEYVELC
                     EPESVYMCDDSDEDAEYLRKRSLELGEEKKLGKEGHTVHFDGYHDQGRAPGATKNLVR
                     RELLAPMKALAAKDRDEGMAEIREILRGIMKGKEAVVKLSCEGPTLSPFSIGCAQISD
                     SFYVCHSEEILYRRGYEHFMGMEDKDRFFRFIHSAGELDDRGNSVNLDKRRIYQDLED
                     MLVLSANNQYAGNSVGLKKHAMRLAIRLSGQEGWLCEHMFIMAVQNPPDGRSTYFCGA
                     FPSACGKTSTAMLPGEKIVGDDIAYFRNIGGEFRAVNVERGIFGIIKDVNPEDDPIIF
                     KTLQTPGNEIIYSNVLVGPDNAPYWLGMGTEVPEKGTNFSGDWYRGKKDELGNEIPFA
                     HSNARYTLRLMDLENIDHNYKAKNGVWVRGIIYGGRDSDTSVPIEESPDWESGIILKA
                     CTLESETTSATIGKEGVLAPQPMANLDFISYPIGQYVLNNIHFVRGMENVPRIYTVNY
                     FLKNSRGQFLTSKMAKKVWIHWAERRVHGEFGAHRTPLGWIPIYEDLAGMFREFLEED
                     FSRELYDELFKFRIDPWIAKLNRAIAFFEKSAPDCPEKVYSIWKAAIAKLQGAKSRYG
                     ACIPPGTYRES"
     gene            complement(33459..34301)
                     /locus_tag="PLM79_03175"
     CDS             complement(33459..34301)
                     /locus_tag="PLM79_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85335.1"
                     /translation="MIVRCYGARGSIPVSGKEYFRYGGDTCCLEVRSRNDEIIIVDTG
                     TGVRRLGKKLILEGRSEFNILFTHSHWDHILGFPFFKPIYSDKTILHLRGCPTAQGNI
                     QRLLSKAMSTPLFPVPFDKLNARILYDGSCPSILTIDSIQIHPIHLSHPNVGLGFKFV
                     EKDTQFVFLTDNELGYRHKNGRSFEEYVEFARGSDLLIHDAEFTPQEYAFTRTWGHST
                     YLDALDLAMAAGVKRLGLFHHNQDRKDEDMDRLVRHCREIADKKGTAPECFALTQDTE
                     LVLE"
     gene            complement(34633..34794)
                     /locus_tag="PLM79_03180"
     CDS             complement(34633..34794)
                     /locus_tag="PLM79_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85336.1"
                     /translation="MAQHSTEEKMFTCAACGEEYTRDAAVSECRMCRRTFCEECIDEW
                     GNCTPCGQE"
     gene            complement(34982..35344)
                     /locus_tag="PLM79_03185"
     CDS             complement(34982..35344)
                     /locus_tag="PLM79_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85337.1"
                     /translation="MWNLEELQRHREIIDQIDWDMTPEKAVETYLEWGTGWARKDDFV
                     RYPGQASYYFLVYGWERPLCVTLVRRDVSQMVEIARVEAPEELILEAVAEGGRKPGVE
                     VYAVSEPLKEWLKRSLNC"
     gene            35864..37816
                     /locus_tag="PLM79_03190"
     CDS             35864..37816
                     /locus_tag="PLM79_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV85338.1"
                     /translation="MRIRLGLQAKLILGGIGFMLTPMLIVSFFSTIKASSAMESISRN
                     GAANQAQSTADMIQRILLEEIKLATDLSVGNTTIDVGTKMAREGHDGSSQEVEKLSRK
                     LTEAMKRIGKDYETILVADQNGLVYADGCGGASVGTTVQSTDYFGGVRSGKVTLGSVQ
                     PSIVTKEPVLPIGAPVFSKEGDFVGCLVVVLKSDFLNGQIADTRVGQTGYVFLVDKGG
                     RIIAHPKKDLILKVNISSVKGMETIAEKMRQGHSGVEEYSVEGIRSLAGFAPVKLTGW
                     SVAATQPLSEGMTLVQSVRNSILVMAGIFLLAAFLVMVFGARETAKPIRFAAEGLGEG
                     AEQVARASMEVSQASQQVARGTLQQAASLEETSSSLKQMSSMTARNAENAARANAYME
                     EAMTTVDEASRSMAGLTASMQEISGASEEIGNIIKTIDEISFQTNLLALNAAVEAARA
                     GEAGAGFAVVADEVRRLAVRAADAAGNTSILIEKTVRKVREGFGLVNQTNTEFRRVAD
                     SVAKSGSLITEIAAACREQSQGILQINQAVSDMDQVVRSNAAGAEQSAAAAEQMNAQA
                     RQMEEFVGGLVALVKGERGNGDRGERTALYACEGSFPGLDPGSPGSRISAGRLSSADG
                     NSRGTLPGGDPRSLADRSGCLPGNRD"
     gene            complement(38447..39037)
                     /locus_tag="PLM79_03195"
     CDS             complement(38447..39037)
                     /locus_tag="PLM79_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV85339.1"
                     /translation="MTGNKTVREIMSPIEDYDMVGADDRLCDALKIIRHNYDKIQAGE
                     PGRYHKTLFVKNAAGEIVGKLSMYDLIRGLVPESAKKADISKTLQRTISVKAAEAAEE
                     IAEVQERMQWLETDFFELVHKQSRKKVRDVMSPLHPLLKEDDTINRAIYMMFKENIRQ
                     PMVVREGRIVGVVALMDIFPELLEIAGDVCFWQPPE"
     gene            39456..39686
                     /locus_tag="PLM79_03200"
     CDS             39456..39686
                     /locus_tag="PLM79_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85340.1"
                     /translation="MVLGRSLRMMLLFSSVLVPPCPAKQGKAGNPRDSAAIRGVVEGL
                     HKSFNAQDLKGVMMAFSSDPHIVLMGTGPGEV"
     gene            39687..40016
                     /locus_tag="PLM79_03205"
     CDS             39687..40016
                     /locus_tag="PLM79_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOV85341.1"
                     /translation="MGDEAVRGAFNQFFNRFEANALNFRYDWVVFASRGGSGWFAGTT
                     TMEAVVKGEKKERSFNMSGNLRKEKGKWRIVAVHISRLRAEEQPAAGMGSASGTGAGG
                     TRLLGAA"
     gene            40035..40811
                     /locus_tag="PLM79_03210"
     CDS             40035..40811
                     /locus_tag="PLM79_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85342.1"
                     /translation="MNDTLVQNVPVERIEAYRGRRIIVRWQDSERIDPGLTDNPESIA
                     YLQVLGTGGNLDELFRWEQPVPVDLVLEDLEKELPLLYRYSPLSARHPVRVFIPVTSG
                     FSRAVKLAASLDFAVKLEPSQPDPQRMGELFRVVDFYLHQWTVSQPVEFIHSMFPGFS
                     HGSPFTLWAVQEEDPSRFRFVTREGEETVSRKFAGVEPGHELPSFIAGFSRELLEDAG
                     ECCDCRSFEVCLGYFKWPEKDYRYDGVKRPFDRLEKTAED"
     gene            40856..41335
                     /locus_tag="PLM79_03215"
     CDS             40856..41335
                     /locus_tag="PLM79_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85343.1"
                     /translation="MMTRGPLSLILLPTLLCDADCEFCIQKKTDGQLTHDELSVVVRK
                     VVDHMVREHIETLSVYRQGGEVMTPAPEWFEEAHGIIGEAVNSGRRRVEHFVQSNLIA
                     YSDRRDWVLAQMFGNSVGTSMDYPGLHRKLKRGVRRNTTEYGRGMFERQEMPASKSG"
     gene            41332..41979
                     /locus_tag="PLM79_03220"
     CDS             41332..41979
                     /locus_tag="PLM79_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOV85344.1"
                     /translation="MIPIPNERTLELGAERSHSHFTDELGIQDFQVNTPFPGGASNAV
                     KPGYPLETGRLSEFLVALARVWLERGFERGVRVGPFDGLVRYFVHGGKDFLCIWRDNC
                     ANEFVCIDPRGHVAQCDCWATSYPESRSGNVFDSGSLSDLLRNSPARLRIQSRPGVLI
                     RTEDCLDCNSLSIRHGGCPVRAYSGYGDLFRKDPYCALHGRIFPAVEELAARYVS"
     gene            42055..46080
                     /locus_tag="PLM79_03225"
     CDS             42055..46080
                     /locus_tag="PLM79_03225"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85345.1"
                     /translation="MTAERPSGNTGGNEEPLERIRGMLKSKSAEGVGRGGATEGRVPP
                     YGDLTLLNTSRLILDSVGSSFLEDIFRDFTELLGTTCAVYEKNGDYALRMFPPGWCRF
                     MDQASRDGCLTADNREALDSGQWHCHESCWKEAALLSIRDKGPVDVACRGGIRLFAAP
                     ILAGGEAVGAVSMGYGDPPRDEARLNELANAYGVRKEELRRHADAYPTRPPFILDWAG
                     KHLVSVARWIGEIVERRRAEQALHRSEEEFRALLDHAKDAVLIHDAQGRIREANLAAC
                     ERLGYRREEFLKMTLLDLVDPEQTAQVPQRFNTLRKQGHHFFESVHRRRDGTAVPVEV
                     SVRRLERAREELFLAVCSDLTERKRAEAALAEVEKRFSVFMEHLPAGVFIKDPSGRLL
                     FANRYLKELFDWGDCVGKTTWELVPRELAERMVADDRKALNEGPLVIQETVTDPRGTE
                     HFFETYKFPVEVVGSPPLLGGITVDISKRKRAEDALEKRILALTQPLDDAGNISFEDL
                     FNLADLQHLQDLFAEVWGVAALITRPDGTPITRPSNFTDFCSKFIRRSEKGVHNCQVS
                     DATLGRHNPSGPIIQKCLSAGLWGAGASITVGGRHIASWLIGQVRNEAQSEAQILEYA
                     RLIGVDEGAFREAFLRVPIMSERTFEQIAHSLFALANQLSNTAFQNIQQARFIAQLKR
                     AEEILRESEAKFRGICEESPIGIELYDRQGTLLHANRAAYDIFGVSDPSTVKKFNLFG
                     DPNLSEELKARLRRGENARIEIPFDFERVRALGLYETAKSGVVHLDVLVTPLKEVAQG
                     PVIGYLVHIRDITERKRAEEALRDSEARYAVVVEQAKDGVVIIQDDVLQFVNRSVGDI
                     LGYAQGEMIHTPFSDYVAPEYRPMIAQRVRDRLEGKEVPSVYEAALVRKDGTRIEVEL
                     SANIIQYGGKTADLGLIRDITERKRMEEERRRFEAQMREVQKLESLGVLAGGIAHDFN
                     NLLMAILGNADLALASISPTSRAHHHVEEIARASLRAADLCRQMLAYSGKGRFMVGFY
                     DLSEIVREMAQMLDVSISKKARTRFSLTPDLPAVEADATQLRQVVMNLITNASDALGE
                     EGGVISIATGVKECHEAYLSEGYLDDGLPGGTYVCLEVSDTGCGMDAETLSKVFDPFF
                     TTKFVGRGLGLAAVLGIVRGHKGAVKVRSEVGEGTTFEILLPAVAWESGGKAGVAGPG
                     MSLQGGMQGGGTILLVDDDPRVRSVASEMLVQLGFQVLGAENGREALDVFRTRRAEIA
                     CVILDLSMPVMGGEEAFRELRNVSGDVPVLVSSGYTEQDVAQRFAGRKPTGFIQKPYT
                     IANLRGALEEALRPPEDPRPA"
     gene            complement(46230..47150)
                     /locus_tag="PLM79_03230"
     CDS             complement(46230..47150)
                     /locus_tag="PLM79_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85346.1"
                     /translation="MTKPTRLRLWGKLRGAGDRGSVRALTAVMTRGLCLGLLAVGLIP
                     HPVAAVETAAQPSNPSLAVSVSEVPTAVSAYEAHHEVASHPKRASFGQEPKSRDTQQV
                     ADWVVDSGDNLGLTFAIIDKVNAKVFVFDADGRLLGAAPVLLGLARGDESIPGIGQRK
                     LSEVRPEERTTPAGRFVAAMGYDPKGKEMLWVDYESSVGLHRVVTTKPAERRLERLAT
                     PTATDNRISYGCINVPANFFDSIVLPAFTGTSGIVYVLPDTRSIREVFKSYYDVELRR
                     GAGTAANRDETLGPGAQQQDLRIGRHEARP"
     gene            complement(47914..48603)
                     /locus_tag="PLM79_03235"
     CDS             complement(47914..48603)
                     /locus_tag="PLM79_03235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV85347.1"
                     /translation="MKIFLPKGQSMNYFCRFVEYFDHNYQFFRQQIALINRLEPMAVV
                     GGTDSLSTDVEFIYVPLNFSIRPKILPERIESISKLPQFILIDIGQQCLGLYEYGKLV
                     HLFPISSGKNGGTPARKFVILSKEKDHYSTKYDNAWMPYSLRLFGNYFLHGGILPTYA
                     ASHGCVRLIYQNAVFVYNWAKVGTPGEIVNETELDVDDPAPVQVVSRATSRQEEGVPE
                     EEEEADIKSLY"
BASE COUNT        10136 a        14028 c        15320 g         9808 t
ORIGIN      
        1 gacacggcca cattgcccgg ttggactacc ggcaacgcaa ggatgtccag gtggacgaga
       61 tcatccccca gctatacgtg aatgtcctgc ccaacatctt cgttaccacc tatcacgact
      121 attctatgga caagggggaa ctcttctccc agggctacgg ggtgctctac cagaaagggt
      181 gttggggact gggcctgatg tacgagaagg aagccaagga tcagcgaatc gccttcacca
      241 tcaacctgct ggggctgggg agcataggcg gaagccgaac ctaccgggga ggcggcaacc
      301 ttcccagcgg ctttccctga tgccgtaggc tccctgcagg gtcgtttcga aacgcttcat
      361 tccttccaag tcgccggatg aggaacccgc gatcgacagc agggaaaacg agagcccccg
      421 acggtttccg ttgccccgtt catgccctgg gacttctcct gccttcgaag cggaggtttc
      481 cattccctcc cattcccgtc gattgtcccc atcggatctt gacacttgtt gtcccggctg
      541 caatgataac ggcggatcgg gtatcagtag aaaccgcctc tttctgtatc tccgcccacg
      601 aatcgtgctt cccggcaacc cgtcgatcga tggcgctgcc cggagtctcc ggtcgtcccc
      661 atgctgcaag ctgaatttcc cggtggtgag tctccaggga gcgtcccgcc caagggtgct
      721 tggatcatcc cggggcctct cgtcggacac cgggaagagg acggaaggaa ctccttgcgg
      781 gggaaggaaa gcacgttggg cggtcgtttc cccaaaccca accctcggtg aatccatggc
      841 agagggactc aacccaggat gacaagaacg gacatggccg agattcggga aactctcttc
      901 cagcgggttt ttgacagcct cggggaccca gtcgtggtct acgaccggga tctgagaatt
      961 ctggacgtga acgtggcgat gacggaaagg ttcccggccg cccgggagga aatcctggga
     1021 aagccttgcg gggagctctt atacgggcga ggaaaccttt gtgaaccgtg cggcgttcga
     1081 caggccttcc acacgggaag gcccttcgtc ggggaaaaga ccgtttccct tcccgacgga
     1141 aacacccggg tctgggagtt gaggattttt cccgtcaagg gttcccgcgg caagattcga
     1201 gaagtggccg aggtggtccg ggacattacg gaacagcgag ggctggaaaa ggagctccgg
     1261 gattccgaag aacgatatcg gaccatggtg gagattgccc gagaggggat ctacattgtg
     1321 gacgccgaaa cccgggtgac ttttgccaat caatgccttg cccgcatgtt gggctatccc
     1381 atggagcagg tcctggggcg ttctctcctg gaattcatgg acgaagaatc ccgtgggatt
     1441 gcccggcaac agttcgagcg ccgccgccgg gggctttcgg acgtgtacga attgcgtctc
     1501 accaaggccg ggggggcgcc catgaactgc ctgatttccg tggcgcccct ggtggacaac
     1561 ggttctttcc ggggggccgt agggctcatc acggacatca cctgcctcaa aaggaccgaa
     1621 gccaagcttc gggccgccaa ggccttcagt gaaaggatca tcaacagcat tacggacagc
     1681 ctggtggtca tcgatccccg aagtcaccga atcgtccagg ccaacgagtc cttcctggcg
     1741 agggttgccc tggggagcct ctccgagctt tcggacccca cctgttttcg ggtcctcctg
     1801 ggaagggaag agccctgcga tccggaaaag accgggtgcc tggtgaggga taccttcgag
     1861 agcaaacgac ccgcccgggg ggaccgttcc tatgtggatg cacggggaac tccccgggtg
     1921 ttcgaggtga ccacctaccc cgtcatgaat tcccaggggg aggtggacct ggtcattcgc
     1981 ctcgatcacg atgtcactga aaggcgcaag atggaggaag ccctggcgaa ccgttccctg
     2041 gagctgcaaa aagcccagca gcagttggag gccctcatcg acatttcccg gcgggtgagc
     2101 accaagggct ccatctccga gaccctccaa tacctccacg gggtcggtcg aaagatcttt
     2161 cctcaatcgg attccctctt tttcatcctg aatcccgccg gggatcaatt tctcaggctc
     2221 gaagaatgtc cggccgcctt gtcggggccc cttcatcaga tgatggaaca aatctcccac
     2281 ggggggctat tggcggactt ttgcgatttc ctggccaaga acaagcaaca gcaaatcgtc
     2341 catcatgagt cggacaatga aaagcacccg gttttccgga tcttctccca agggtatccg
     2401 agctggttcg gagtgtttct ttgccccaag aaccagtgtc tgggtctcta cgtcctgggt
     2461 tcccgatcct cccgggaata tccccaggaa gacctccggt tcttccaggc acttttcgaa
     2521 caggtggcgg gacacattcg tcacctggtc cagcatgaaa ccgaagtcca tcaactgaag
     2581 gaacaggcgg cggagcgggc gtcctacggg gagatcatcg gccggagcaa gaagatgcag
     2641 gaggtctatg agctcatcga cctggtatcc tcgtccgacg ccaccgtgtt gatcacgggg
     2701 gaaaacggga cgggaaagga actggtcgct cgggccattc acaagcaggg gcatcgttcc
     2761 aagggcccct ttgtggtggc caactgttcc gcctattcgc cgaccctgct ggagagcgag
     2821 cttttcggcc acgagagggg agcgtttacg ggtgcgattc accagaagaa gggtcgcatc
     2881 gaacgggccc atgggggcac cctctttctg gatgaaatcg gggacatcgc tccggccacc
     2941 cagatcctcc tccttcggtt cctccaggac cactgttttg aacgggtggg aggggagaag
     3001 accatcgagg ccgacgtgag ggttttggcc gccaccaacc gggacctgct gcaggaggcc
     3061 cgggggggtc gatttcgaga cgatctctac taccgcctca acgtcatctc cattcacctg
     3121 ccccctctgc gggagcgcaa ggaggacgtg ccgattcttg ccgagcattt tctcaggaaa
     3181 agcaccctca aggagggcaa gaagattcac cggttttcct cggacgccat gcaggctctc
     3241 atggatcacg agtggccggg aaacgtccgt caactggaaa acgccgtgag tcattcggtc
     3301 atcgtggccc gggaagacgt catcaagcgc aagcacctgc cccggtttct gagggagggt
     3361 cccgagggtc cttcttccac ctccctggcc gagaatgagc gccagttggt gttgagggtc
     3421 ctgagggaat ccgactggaa caagcacgag gcggcccgga gactggccgt gagccggagc
     3481 accctctaca gcatgattcg gcgctaccgc ctcgaacccg ccccgggcgg cagccgggaa
     3541 atctctgggg tcgattcctg aacactccaa gtttcccttt gggatgggct ttaagagact
     3601 tgcctccttt catcgggtcg aaaaccatac acctcagttc ccgaccgatt ccttgagcga
     3661 cttttcacag cgggggaagc ctcgaggagg aaatgctttt cccgtggggt tttgcaggcc
     3721 tcgagcccgg taggacggtg tcttctcgag ggcccagggg accggttctg gcaaagagct
     3781 tgcatcaaac ccggtttcgt atgcagtcca cttgaacctg accagcaatg ttcgtcgcga
     3841 actcatggcg ccgggatgcc gggcttcggg caaaccgtgg cccctccaag gaagttgcgc
     3901 tgccggatcg tcctccgtcc tgttcgactc cgttcacttt tcccgcattt cccatttgtg
     3961 agggctgagt cgtgtcgcct ttggcgcaaa ggcggcgtgg ctgcttatca cgggtcttgg
     4021 ccccattcca aaaccttggg ccgggccgac gggccttggg gcggacggtg agctcgtctc
     4081 acaagactgc cgggcaggca agggtctggg ccgcgcccca tcgtcgagag cgattccacg
     4141 atcacggaag ggaggtgaga gagtcattcc agcggtaggt tgaccccacc gggaacgccg
     4201 ctccccgggg gagcggcggg catggcttgg ataccgggct tgacctatgg gacatgccgg
     4261 gtgttcggag catccgggtg ccgggcggga cgcccccgga cgtttccgcc cgcatcgaaa
     4321 acggggaact gattggcaac tttgagacca cgggaggtct aagaatggca gaaaagaaag
     4381 aggtcgaaat caacagccgg gccgacaccc ttcctctcat gagcccagag gtgaggcctt
     4441 ggcccgtgac gccggctcca tccccggaag aggtggaagc catatacgcc aggcgcaagg
     4501 ccaccgaatt cggcaaatgg ctggaagaca atctccggct ggaaaagagc tggggaaagc
     4561 ccgaagctct ccaggggatg aaggtcctct gtatggggat gtggaggatc ggcaataagt
     4621 tcgccgcctc tcagttggcg gaatcgggag ccgaggtgat caacatcgaa cccccgggag
     4681 gggatccgct tcgaaagctg acccccttcg gtcgggaaga gtacatgttg gcggaccggg
     4741 acacggggga gaaatgcggt ttggatttca tctccgaaat gcgaaacatg cagtccatca
     4801 ccctcaacat cgagacggaa gagggccggg aggtgtacag gaagctcgcc acccacgccg
     4861 atgtcctcat cgaagagtat cccccggggt acatggacgg gttgggggtc ggctaccgtc
     4921 agctctccaa gatcaacccg agactggttt actgccagat ggcggtgcgc ggcacctggg
     4981 gtccgtacaa ggacaagttg tccaagcacg ggcagtggac cctcgagccc ttcggccaat
     5041 gcgccaactc cttcgtccac aacaccggtt tccctcagga ccagttgccc aggggcaggg
     5101 gaggggaccc cacccgctcg ggagtctggt ttgccgatta cgtggcgggg gaacaggcgg
     5161 ccgtgaacat tcttgcggct ctttattacc gagaagaggt cagcgggaaa ggccagttga
     5221 tcgaagtcac cggggcggag gcccagatgg acatcctgga cttcgacatc ggctggtacg
     5281 gtttcaacca gagcatcaag gccaggactg gaggctggga cccgaacctg aaccagtatg
     5341 aatggaatcc ctgcaaagac gggtacatga tgatcggcgg ccagacggac cgtctgtggt
     5401 accgcatcgg catgtgcatc gagcgggatt ttccccagtt cggccgactc attcatgagg
     5461 accccacact caaagaaatg ggcgccagga atgcgctcca ggccctcatc aagacctata
     5521 cgctcaccac ccggtggctg agggacacca accgcatcga ggcggagcaa aagctcatgg
     5581 aatacgagat cgcggcgggt cccgttctgt tcatcgacga ggtgtgcgaa ttcccgcact
     5641 ttgtgtaccg tccctgggtc tataatctgg acagcgagca atacgggacc gtgctttatg
     5701 ccgtaccccc caatgcgtac cagcagagga ccccggcgcg ggtcaagtgg cttggaaggc
     5761 ccctgggcaa agatacctac gaggtctgca tgaagtacct cggcatggga caaaccaaga
     5821 tcaacgaata taaggacaag ggggtgatgt aatggccaag gacctgagag aggtgaggct
     5881 ggaggacctg ccgggaccct ccagcaagga agaactggaa gagatgaaaa tgccctatga
     5941 ggactactgc cgaaagatgt tcgacgtggg cggcgaattc ggcaagcctc aagccctcaa
     6001 gggcattcgg tggctgagca ccaccatgta catctttacc ccccactccg tggccaacct
     6061 ggccgagttg ggggccgagg tcatcaagat cgagatgccc aggatgggag accccatgag
     6121 gcatacgtct cccttcaacg aggcctacct gtatcctctc catgactcgc gccccatgac
     6181 gggaacggga tttggcttcc tgggagccaa cgacagcgag taccacatca ccatggacta
     6241 tcacctggac gaaatgaagg aagccttcta tcgcctggtg aagatgtccg acggcctgac
     6301 ggaatgctac cggcacggca ccttcgaccg gtggaagcaa agctatcgat acctccaaga
     6361 gttgaacccg cgattcatct acgtgtgggg aggcgggttc gggtacggcc ccaagatctt
     6421 cggcggttcc tacgacatcc tggggcaggc tcatgcgggc ttggcctcgg tcacggggat
     6481 gcacgatttc atgggaggtc acgtggccaa acattccaac tggtgcatcg actggtactc
     6541 ggggaccctc atcacctacg ggattcttgc ggccatgcat tggcgccgga aaaccgggct
     6601 ggggaccatg atcgagttct cccaggtcca ggcggccact cgagccctgg gctacaccgt
     6661 gcccctgtac ggaaggtttg ggatcgtacg tcagcgatgg gggaactggg atactcaact
     6721 gtgcgtgcac ggcatcatcc tgtgcggcaa gtcggacttc ccggattccg aaaaccccca
     6781 ggacaagtac gacgcccggt acgtgatggt cagcgccttt caggacgccg acttcaagga
     6841 actgtgcgac atcacgggac tcaaggagct ctacgccaag tatcaggccc acaaggatcg
     6901 ggtggaagcc gagccccagg tggagatcta cgcggccctg gagaaatggg cctcggacaa
     6961 gagccgctcc caggtggtga aaatcctgca agatgccggc attctggccg aacccgtcat
     7021 gaacgaccgg gaggtctatg agtgtgaaca ctatcgacag cgaggaacca tccggtggca
     7081 tgaagacccg atcttcggcg acgtgctttt gcaaagcaca tacagcacgg gcctcatgtc
     7141 cgaaactccc aggaggcaga gctggatctg gcggccggtg ggtgcggaca acgtgaagat
     7201 ctaccacgag cttatgggct accccatcaa gaaggtggaa gactggtacg ccaagagctg
     7261 gatatagaag gaggatgcgc tgtgtgtgat atcatctggt gcaagaagga gatcgacgga
     7321 aaggtctgca atacggtcaa ttacctggat ccctactgct tctggaactg ggaaggaaaa
     7381 gtgaactgtg ccgagtgcgg gacggtttac tacattcaca tgatccaggg cttcatgtac
     7441 aagggtcccg aggaaaaacc cggggaaaag cccgacacca gccccctggt ggcggaccag
     7501 cccctcaacg gctacaagtg tcacatccca ggaatcgaag gaaggacccg gccctaccag
     7561 tgccttcccc gggggattta cctggggcgg gcggatatgg tcaagttcag caagagaggc
     7621 agactggtga ggggctggag accgcagccg ccctcctcgg gcattgccgg gagcttcggc
     7681 ttcaactggg atgccaagaa gttgtccccc gatgtctggg aggaatacca ggagaaactc
     7741 cggaagggcg aggccaagga ctggtagcga agaaaggagt gttgcatggc acagttaacg
     7801 gcgcaagaat ttctggatac cgtattggtt ccggagaagc aaagtgacca tatctgctgg
     7861 cagtgcaggt ttttgaggcc cgtgttgaag aattcgaagt tccctcccgc agacatcatt
     7921 ggctggtgca ccaagctcca ttggcctcat tactggtgtg tcgccgactt caacgtggtc
     7981 aagaagtgtt acgcattcca gtcgaaacga taagcggtat ggaagaacaa atttccagag
     8041 agctggaagc aatcaagaaa atggtgcaga cctggaaggg gagctatctg cgggaagctc
     8101 ccgaaaacgg gaaggccgaa tatctggtgg aagacctctc ccaggagatt cggacctttg
     8161 tccaccccta tgtgcgcagg tttctcgaac tgggctatct cagcgagtcc gaggcaaggg
     8221 agttccttga cggttgcgac ggcgaagtcg aggaccttcg cagaatcgtg gggtagggtc
     8281 cggttcgagc aaggagaggg tgtgggggat tgcgggatcc gggaggcttg gtcctcggga
     8341 tcccctggat ctctcagtct ttggaatcgt ttaggaggat gagaatgcct gcaaaatgcc
     8401 gtgtggtcgt ctgcgggttt gaccccatgc tcgtcagggg atatgtgaag acggggtaca
     8461 gagccctttg gtggcatatg cccgatgaaa tctgggagga acacagaccc cttcgtggaa
     8521 agacggtttc gggcaaggtc ctggccgtct ataacgggga tggtgccaaa acggcgtctc
     8581 ccaacgagca ctacgaatgg aacacagcca aagaatccgg cctggcggtg gtcatcccgc
     8641 ccgaggccat cgtcaagtat cagctcaccg agttccactt tcttgagctc accatcgagc
     8701 acgtaggcgg aagagacgtc tatcccggcg aggagaagat gagctccaag tggtggcccg
     8761 aagagaaaat gaagctcgac tacaagctgg cgtatgtagc cccctagagt cgagggaata
     8821 tcccgccccc cctctcctca cccccacccg ggaggaagag gggggttcca ttcttttgcg
     8881 tctccgcgaa ggattccccc ctgtgcggga aggggcgacg ccgggaacct cgatgtggga
     8941 tcgagaagag ccttttccac cttaattcca gccgaaggat gcagagcgat tatgattacc
     9001 gagcagggcc gcgacgccgg aaaagagtcc caagatttcc aaacggatcc gcagcacccg
     9061 gggtggcttc ccctggattc catcctgaaa acgggcgaac cgatttttca gcgattcctg
     9121 ttcatgctgg gattcgatat ttcgtccaat atctaccttt ctacgggaga gagcctggct
     9181 gttgtggatc ccggcaacga ctacaccgcc tttatggagc ttttcgggaa gggatacgcg
     9241 ccggaagcgg tgaagaaagt ggtgctcacc cacggtcacc gggaccattc catgggagtg
     9301 ttcgagttgt tgagggccta tccgggcatc cggaagagcg gaggcttcga gttggtgctc
     9361 cacgaagccg cccccccgga gttgaagcaa gtcgctacgg agttcggatg ccgggtgacg
     9421 gaagtccggg gcggggaaac ggtacggctg ggggacgccg agtgggaggt ggttcatacc
     9481 cccggacata ccatcgacgg gatatgcctg gttcatgccg catccaagac ggcgtttacg
     9541 ggagacatgg tgcagcccca ccttatggcc gagccggaca agaacgccgg tggggatctg
     9601 aaccattacc tgtacggggt caaggaactg ctcagaaggg agatcgaaaa cattttgccg
     9661 gggcacggag cccccatcgc ttccctgggc cgacgcatcg tggaggagac ctatgtcggt
     9721 cttctcatga ggatcatcgg ggcggaagcc cccatcccct ggatggacgg ggccatggcc
     9781 ctattgaaga aggggcttcc ggaagagacg gttttttgcc tggagaagga attggcccag
     9841 agacccatga atccccgggc tttgcatgtg aaagccctgt gtctgaacga cctggggcgc
     9901 ttcgaggaag cgctccatac cctcgatgaa ctccaaggcc tggaggcgga ggaacccgaa
     9961 ccgttctcca tcctggcccg agggtacgct ctcatggggc tgggccgcta tgatgccggc
    10021 atcgcttttt ttgaccgggt gctgcaaagg gaccctcgga tgcaggacgc cctcgtgtac
    10081 aaaggcatgg ccttgtatct ttccgggcgc cgggaagaag ccatggagat cgctcccttc
    10141 cgaaatgagt tcgtgggcag gttcaaggaa gcgatttccc gcaccgccgg cgtacacgga
    10201 cccgggcccg gctaggtccc catggcgggg ataggttgtc caacggtggg aaccctgcgt
    10261 cccccgggac ggctcctctc cgttggcttt cggagctcga taccacggac ttgaggcggc
    10321 aggtatttgc agacgatgaa ctcagaaagc gatcatccct tggtgcttct gcttgtttgc
    10381 aacaaagaaa ctcgcgagta cctgtcggat ttgctgcgga ggaattccta ttcgcctcaa
    10441 gccttttcgg ttcccgcaga cctcctcaat catttgcgag ccgggacgga cgccattgtg
    10501 ttcgtggacg gccaggcggt gagcaattgc ggagcgggga ttttctccaa gatccgggaa
    10561 tcctgcagcg gatgcaaggt catcttcctt tgcgatcgtg cccaccgtga gctcatcaag
    10621 gaagcgatgg gcctgggagc ctacggctgc gttctggaac cttatgagga gtgggaagtg
    10681 ctgaccatgg tccggcatgt gctgtcggac ctcaagccaa aacgtcgcag gagttccaag
    10741 aagacccgga gacccgccga aggatgaaac ggaccccatc ccggcctagg cccagcaatt
    10801 tcctgaaaac ggggccccgg ctcagtcgat gcctccagcc gcaaaattcc cctcgtgata
    10861 tcctggcggg cgcactcgca ggaatgagaa atgccccttg cctgcggcag gagcgtacac
    10921 acctcgtctt gttccgatcg acccgcgttg agaatggaag ccccggtgga cccggtttcc
    10981 ttgcggtaac ggatgaggtt tcccctgccc cttcaaacac tccaatcctc ccggccggga
    11041 acatccttcc gcgacagggg gagaagaagg ggagcttcct tgagctgctt ttttgcgatc
    11101 actaccaggg accggatcta cctggttgcg gacaaccgtc gcagaatctt cggccgtgcg
    11161 ggagaaaggg cgttcgacgg ggccgttctc ctggatgatg cccgcaagat ttgccgcgtc
    11221 tgcgattcct tctgggttgc ggggacgggt ctccacgaat tcctagtgag gatggtggag
    11281 gaaatccgaa gcgcatggat tccaagggag gggcgggagg ggccggattt aaaggatctg
    11341 ttgacgcttc gaacctggga agaaacatgt cggaaaatcc atggggaagc cgtgaccgaa
    11401 tgccgtcgcg catgggagcg gacgaaggcg ggggtctggg accccggcga atcccggggg
    11461 gagatcctcc tggcattcct gccccaggat cgtcacccgg tcctccttca tgggaagtcc
    11521 ggggaggatt tcgtcctttc ccggcactac ggaccgggta gggtgatcgt ctcttctctc
    11581 tcttcctcgg gggacggatt tccggaccga gagctttcgg ccaggctgga gtgggtggcc
    11641 cgggagctgt tgaaatcgga cccttcggac gaaatccgcc gagcctacga cctgttgcct
    11701 ccattgttgg gctttgtgtc cgaaatcatg ccccgggagg tgagtgcctg cggagacctg
    11761 gtggtgattc acccgggcgg gtacgagtgg tggttgttct gaaacgagct caaggaagaa
    11821 ggggaccccc ggcttgttgg atcggcggcg aaggggcggc accggacctc gagagagcct
    11881 ccgggggacg acccaatggg agggagcctt ccgtgacctg gacagcggtt gttttttggg
    11941 atactcaaga ccccgccgat gtgggttggc gctggaaaat tcaggacctg gaaggaggaa
    12001 cctgcctttc gggtgccgcc gcctccgcgg aagaagggat tcgccgggta aaagccctcc
    12061 tgaaggagaa agggatcgat ccggggtcgg tgtggatgga tatcctggat gacggcgtgt
    12121 gggacaagtg ttgaggaaca cgaatcctgc ccatccccaa agaagcggct tgacaagggt
    12181 cggccgattt tttatacatt actgcttcaa ttttgaaatg ttcctcatgt ctggagaccg
    12241 agcaatgaag acgatgagtg ctaagttcga agccacgggc cgaaaatgga ttctggtgga
    12301 cgccgaggcc caggccttgg gcaggcttgc gagccaggtg gccgtgaggc tgcggggaaa
    12361 acatcttccc cacttcacgc cccacgtgga cgtgggagat tttgtcgtgg tgatcaacgc
    12421 cgagaagatc cggctgaccg gcaacaagtg ggatcagaag gtctactacc gccacagcgg
    12481 gcacatgggt gggctcaaag ctacgacggc caaaaagctc aaccgggaga atcccgaaag
    12541 aatgatccgc ctggcggtgt ggggaatgtt gccgaagaat cgtctgggaa gacagctgct
    12601 gaagaagctg aaggtgtacc ggggttcgga acacccccac gaggcccagc agccgacaca
    12661 ggtcgtttta ccgacgtaac cgggagagcc aggagacgca aggaatgcca gagcaacgtt
    12721 tttatgctac gggaaagcga aagaccgcag tcgctcgagt gtggttgcag gctggaacgg
    12781 gtcgtgtgat gatcaacaag aagcccgtgg atgactacct cgaccgggaa accagcaaaa
    12841 tgatcatatc ccaaccgctc attctgacgg gcacgtacgg caagatggac gttctggtca
    12901 gcgtgaacgg gggcgggggt tccggacagg ccggggccat ccgtcacgga atttcccggg
    12961 cccttctcga attcaacccc gagttccggg acattctgaa aaaggcggga ttcctgaccc
    13021 gggattctcg ggtgaaggaa cgaaagaagt acggtcgccg aagcgctcgg gctcgcttcc
    13081 agtattccaa gcgttaatcg gttcgccggt cggggaatgt cggggtttct ttttgctgcg
    13141 accccggggg aacctgccgt cccgtgggcg atcgatccgg gaaattcttc ctccttccgg
    13201 ggcccgcccg gatgaggaca cgggctggaa gacaagggcc caaacggact cagggccggg
    13261 atcgttgaaa gaataatgtt cccggagcgt ttccgggaag gatggggaga cacgagaggg
    13321 cgttacacgt ggggtgtagc gccctacgtt tatggactag ggaagatgtt atgctgcgag
    13381 tagccgttgc aggggcctcc gggtatacgg gattcgaatt ggtgcggctt ttgtgcacgc
    13441 atccccgggc aaagctgacc atgatcacct cgcgggctca gaagggacag cggattgacg
    13501 agttctaccc ggcgctcagg gctcattgcg acctggtgtt tcaagacccc gacccggtgg
    13561 aactcgccgc gcgggcagag ttggtttaca cggcattgcc gcatcaggcg gctatggatc
    13621 tcgtgcccca gctgctcgat cggggagctc gggtgattga tttgagcgcc gattaccgtt
    13681 ttatcgatcc tgccgtctac gaatcatggt accaacccca caagaccccc gaactccttt
    13741 cggaggcggt ttacggattg ccggaactgc accgggaagc cattcgcaac gcccgcctgg
    13801 tgggcaatcc cggctgctac cctaccagcg tgatcctggc catggcccct ctcctcaagg
    13861 agtctctcgt ggagccgggc accatcattg cggattccaa gtcgggagtg tcgggtgccg
    13921 ggcggggagt ctccctgggg gttcatttct gtgaggtcca ggagggattc aaggcttaca
    13981 aggtggccga acatcgccat cggcccgaga tggaacagga gctgagccgg attgcaggca
    14041 agcccgttcg aatcaccttc acccctcacc tggttcccat gacccggggg attctcagca
    14101 cggtgtacgc ctctttgaaa gacggggtcg acctctcccg ggtcgaacag gcctttcgtt
    14161 ccttctatca aggggctccc ttcgtccgcc tttgcggttc caaggacctg cccaccaccc
    14221 tccaggtccg gggcagcaat tactgcgatt tgggatggcg ggtggatgaa cccacacggc
    14281 gcatcatcgt ggtttccgtc atcgacaacc tgacccgcgg cgcatcgggc caggccgtct
    14341 gcaacatgaa cctgatgttt ggtttccccg aaaatctggg attggatttc ccgccctggc
    14401 aaccctgagg gttgcacgga gagggaagag tggacctcgt ttgcgctctg cctgacgggt
    14461 gaaggaagtc aaagggtcat gcccaggcgc aatctgcgat gtgtgctcga atatgacgga
    14521 tctcaatacc acggctggca gcgccagcga ggcgtgctca ccatccagga ggtcctggag
    14581 actcgcctgg aaatcatgct caatcggccc atgagcgtca gggcctcggg acggaccgac
    14641 gcgggagtcc atgccagagg ccaggtggtc aatttctaca cggagacgcg catggggccg
    14701 gaagaggtcc aaaagggtct caacagcctg ctgcccgggg acgtggtggt gctgagggtc
    14761 gaggaggttt cggattcttt ccacgcccgg ttttccgcca agagcaagac ctacgagtac
    14821 cgaatcctca atcgcccgac accgtcggcc ctggaaagaa ggtacgcctg gcatatccgg
    14881 agccctctct acctggatcc cgtcctcgaa tccctggaat tgctcaccgg tacgcacgat
    14941 tttgccgcat tcatggcatc gggcagctcc gctcgatcca cggaacggga tctcatgcgg
    15001 gcttccgtgt ctcgacccga tccggaacac gtggtctttc gatttgaagc caacgggttc
    15061 ctgagacata tggtgaggaa cctggtgggg accctggtgg aagtgggatt gggcaagcga
    15121 tcgtcccaag agattcccac catcctgacc ggaaaggatc gccgtgaggc ggggattacg
    15181 gcccctgccc atggattgta tctggtgtcc gtacaatacg atgaatgaac acttcctctc
    15241 atgaagaagg gtaccggaat gaaactctct cagcgggtgg cgcgaatcaa accttcggct
    15301 acgctctcaa tcaacgcaaa ggccaaggca ctggcaaaac aagggatccg gatcatcaac
    15361 tttggggtcg gagagcccga tttcgacact cccgaataca ttcgggaagc ggccgtcgac
    15421 gccattcgcc gtggacatac ccggtatacg gccgtgggcg gcattgacga gctcaaggac
    15481 gccgtcctgg aaatgatccg caacgaatac gggctgacct acgggcggga gaacgtcctc
    15541 gtctcctgcg ggggaaagca cgccctgtac aacctcttcc aggcactcct ggaccccggg
    15601 gacgaggtga tcatcccgtc tcctttctgg gtttcctacc cggatatggt ggaactggcg
    15661 gacgggaagc cggtctttgt ggaatgcccc gagtccctgg gctttaagct gcgggcccaa
    15721 gacctcgaaa aggccatcac cccccggacc cgtctcctca tctggaacag tccctccaat
    15781 cccacgggag tccattatcg cagatcggac atggaaccca ttgcacaggt gctcctgaag
    15841 cacgagcatg tggccgtggt gtccgacgat atctactgcc gcatcctttt tggagaccat
    15901 gagtggtgca accttcccat ggtggagggc cgcctcaagg atcgaacttt catcgtcaac
    15961 ggggtgagca agacctactg catgacggga tggaggatcg ggtatcttgt gggggatcgg
    16021 gacgcggtgc gtgcggcgac ggatatccag agccagtcga ccagcaatcc cacctccatt
    16081 gcccagtgtg cgagcgcggc ggctctcagg gaccacgaat cggaaatccg gagcatggtt
    16141 cgggagttcg aatcgcgcag ccggtacgtg ctgaagaggc tcctcgagat tccgggggtt
    16201 tcttgtcccc agcccgatgg ggctttctat gtctttccca acttcgggca ctacttgggc
    16261 agaaaatacg gcgggaaggt catccgggat tcgggggaac tggcccagta cctcctggaa
    16321 caggcccact tggcggtggt tcccggcggg gcattcgggg aagatcgatg cttgcgtctt
    16381 tcctacgccc tttccatgaa ggaccttgaa gaaggcttca atcggctgga aaaagccctg
    16441 gccgccctcg agaagtgatg ttggaaaaag cgtcgggaac ctttcccgtg acggggaagg
    16501 cccccggtgg tgatttcgaa atctatccgc tccctccccc atggcccgtg ggccatggga
    16561 cggcttgggg ttgcggatgg agctcccagc cttagcacgt acggaaagag gggtgtcgtc
    16621 gcctcctgcc gcggagaaaa gaaccccggc ataatgaccg tgctctcgga ccgcccgtac
    16681 attcctgctg ccttcaattg ccgatcaacc ccttgtccat ggcaaaggac gtcaaggcgg
    16741 atgcgctgtg gagatcgagt ttcttcatga ggttcgcccg gtgcttttcc accgtcttgg
    16801 cgctgatgca caggtaatcg gcgatgtcct tgttcttgta accctcggcc accatcttga
    16861 ggatctcttt ttccctttgg gtcagcgtgt cccaagaggt gctggtcttg accctttttt
    16921 tcccctcgag gtatccctcg atcacctttt ccgaaacgcc gggactcaaa taggactttc
    16981 cggaaaggac gcagtccacg gccaacatga gttcgctgtg ggttgcgtct tttaggaggt
    17041 agccgtcggc ccccgctttc agggtggcca agatgtattc ctcggtcttg tgcacggtaa
    17101 ggaccaggat cttcgtgtcg ggacaggcct tcttgatctc cgcgatggcc cccattccgt
    17161 tcatccgggg catggacaag tccatcagga ccaagtcggg cttgaggctc gaacacaagc
    17221 ggaccgcctc gaggccgtct tttgcctctc ccaccacccc atactcgggg cacgaggtga
    17281 ggagcagccg aagcccttcc ctcagaattg tgtgatcctc tgcgatgacg atcttcttcg
    17341 tgtctcccat ccctaaaacc cctttgtgga aaaagccgat gaagttccat cccggcgtca
    17401 tccgggcaaa tgtcgcctcg atatcgtcac ccgggcagaa cgagggcgcg aatcaagcgg
    17461 acgactggac tcggaatatc gccaagagac ggaagcactc gaatgagaac gggcggatgc
    17521 cgggcgcttc ctgacggaat cctcgaagct cgggggaaaa tactgtcttt taaccccgga
    17581 catacacttt atcctgtatt taccgtttgg tcaatttggc aagaataagg gaaagggaag
    17641 agaggagttt gcgaattccg tatggctttt gattccggag agaaggaatt ccgggcggga
    17701 tattgctgtg aggaaaccgg acatgcattc aaccttgatc gtagaagaca acccaatctt
    17761 tcgttcatcg cttcgagata tcctgctttc ccatttccct cggatgcagg tcttggaagc
    17821 ggagaatgga agcgaggccc tggagaagct caggaaagat atccccgacg tggttttcat
    17881 ggatgtgcgg ctcccgggtc aaaacggcct cgagttgacc cggaagatca aggccgccta
    17941 cggagacgtg gcggtgatca ttctcaccag ctacgacctg ccggaatacc gggaagccgc
    18001 ttttcaatgc ggggccgatc atttctgcgt caaagggtcc accactggag aagaaatcgt
    18061 cgggatcgtg gggtccctgc tggtggaaag gggagagaag ttgccctaat cgcgcggtcc
    18121 ggggaggcat tcgatcatac atttcccctt gtccgccgtc caagggggcc ctcgcccggt
    18181 tgccacagtc cgaagccctt ctccagcgga tggtctcacg atgaccggaa tcccgtagga
    18241 ggagtaagag tatcccatcc cggggaaggg ttcgagagga caaggtatct cagactgaga
    18301 gggggaggat cgcaacaccc ccttgaaatc acgccgcacg gaagcggctc ccgtgcggcg
    18361 aaccgggccg ccggggatcg ttctcccttt ggggaaggag ggaaccccct attgaggtca
    18421 gcacatggaa aggaggggta gggcaatgtc aaacgggatt gtactggatt atctgtcggc
    18481 ggatgaggtc aagatgaaaa tgtccatgac cgggggaatc cttcaggcgc agaaatggct
    18541 ggtggtgtat aatgccttgg tcgatcccag accggttcgt gaaatcgcca tgcacacggg
    18601 gctgtccgaa gccagcgtgg ttcggattct ttcggaatac aaccaacacg ggccgacggc
    18661 cttgagtccc accggaagtt cgggaaagcg gccttcctaa gatcgggacc tcgtgggggg
    18721 attgcctccc gtaagtggtc cgacggtttg tgggtctgca gaaggagtaa cggatgtctc
    18781 gttgggggtt cttgctcgcc ttgatcacgc tgggtcttct ttttgcctcc gattccgggg
    18841 cgtatcccgc cggaatccac ccggacctgg agtcgatcct cgcagggaaa ggggaccggg
    18901 acgagctttc cgtgattgta accctggctc aaacggtgga cctcgatccg tcgggagcgg
    18961 tgccccgaaa agccctcaga ggggccatga tccgggccct gaaacggttt gccgcttcct
    19021 cccaggctcc gttgaaggcc ttcctgagca gggaaggggt ggtggcggtc gaatcccttt
    19081 ggctcatcaa cgggcttgcg gtgaaggccc gaccggacgt tattcgaaaa ctggcgcaac
    19141 ttcccggggt gtcccaagtc ctgccggacc ggatcattcc ccttttcgac acgtctcccg
    19201 tcatcggtcc catggcccaa gagtggaacc tgagtgccgt cgaggccccc cgggtgtggc
    19261 gactcgggtt tcgagggcaa ggggtggtgg tggccaatat ggatacgggg gcggatggga
    19321 atcacccgga cttgaagtcc cgttggcgtg ggggtgccaa cagctggtgg gacccttttc
    19381 gaaagacttc ggctccctat gacgttcagg gacatggaac ccagaccatg gggatcctgg
    19441 tggggggcaa ggccggcggg acggccatcg gagtggcccc caaggcccgg tggattgccg
    19501 ccaagatttt caacgattcg ggaagcagca cctccagcgt cattcaccag gccttccagt
    19561 ggctcttaga tccggatggc gatccggaca ccgacgacgc acccgatgtg gtgaattgct
    19621 cctggggcgt ggtgaactcc cccggccggt gcatcgagga ttttcatcgg gatatccggg
    19681 tgctgaaaag ggccgggatc gccgtggtgt tctcggcggg aaacagcggg gaggtcggcc
    19741 ccgacagcag catcagcccc gccaacaatc cgggggtctt ctccgtgggg gccgtggaca
    19801 gcggcaacaa cgttgcgtac ttcagcagtc tggggccatc ggcctgtacg gggaaattct
    19861 ttcccacggt ggtggccccc ggaaccggga tcaaatccgc ggatcccgtt tccgccggcg
    19921 tccaggggtc ttacgccgcc gtggacggca cctccttttc cgctccccac gtcgccggaa
    19981 ccatggccct tctgctgagc gcctttcccg gcctgagcgt cccgacgctc gagacagctc
    20041 tcaaggcatc ggccctcgac ctgggaaatg cgggagcgga caacctctat gggcggggcc
    20101 tgatcaatgc cttcgaagcg ttgaggtacc ttcagtctcg ggtgacgcag gtgaccctga
    20161 attcggtgaa cgcccaggac gggtggatcc gcgagtcctc gaaggtcccg ggaaccggcg
    20221 ccgcggcgcg ctcggcaggg aacgtcctcg tgggcgacga cgccgggaac cgtcaactgg
    20281 tcggattcct ctccttcgat acctcgatcc ttcccgccga cgccgtgatc acctcggcca
    20341 agatcttcct cacgagaaaa gccgttcgag gaaccaatcc gtttgccctc cttaagaagt
    20401 gttttgtgga cctggcggac gggactttca atactgcggc cctggagatt caggacttcc
    20461 aggctgcaac ccctttccct caagtagcaa gaatgagcaa ccccgctcaa aacggggacg
    20521 tgtccgtagg ggaattgggg gaaacggctc tcagggccat ccctaagggc agtgtcgccc
    20581 aggttcgtgt tcgttttgcc aagacgacca acgggaattc cgtcgccgat caggtcgttt
    20641 tctggggaga tgacggggtg ggttcgcaaa gaccccgcct ggaaatcacc tacatgcttc
    20701 cttagggtcg gggctccttc ccggaacggg cggcttcctt cgtagttgtc gctcgagaac
    20761 tccaaccttc ccaccactgg aactttttcc gaaccgccgt ggattgatag atctcgaagg
    20821 caacatccgg tccctccacc aggaccctca caatggtttg ccgacggtcg ccgtggctga
    20881 cggacccggt gttgcccacg ttgatcacgt cgattccata gtcttcgtgg ggaaatcttt
    20941 tgtgatcgta ggcatgggtg tgggcggtga aaacccctcg aaccctttcc ctgtgctgca
    21001 aaagaacttg ccagagcttc cagtgggaac gcggattcaa gaagaggggt ttgtgcatgg
    21061 ccagaaagac gtgttgaatc gaggatggag gctccagaac agccgggagc cacttgatga
    21121 cccgatcccc gccgaagacg gacccgaaat ccgaaagcgg atccaggacc accaggcgca
    21181 cgtttttcca atcggcaaaa tacgtgacgc ttttctcgtg gggagaccgg acccgggggc
    21241 ccagagaagg cagaatgctc cccaaaatca cttcgacgtc ttcccggttc tcgtggttgc
    21301 ctctcacggg aagaaggaaa ggaggcgggg gacccaacag ccgacggaaa ctttcgtagc
    21361 tttccgacac gggtgtgaag tccccgcaga tcagggcgaa gtccaggggc ggctgccggg
    21421 ggttttcggg agaatgcagt tgggctaccc cttcgagcag gtcgaggtaa gcggcaaatc
    21481 cgtgccggtt gtcccccaaa gcaacgaagg accacttttc cgcggctgcg gggcagggaa
    21541 accacaggat cagggccagg agaaggaaaa cgcaccgttt ggcgtagtct gcttcccggg
    21601 agaaggaatc ggccccgggg accggactcc tctcccccat cacagcagct tccagacgcg
    21661 gaggctgtcc cccagggacc gggcggcttc agccatggcc gcctgaggac ccatgatccc
    21721 cacagttccc cgggctttga ccccctcgat cacctccgca aaggctcgaa agtccgaagc
    21781 ccgggtggcc aggatctggt ctctcatgac ctggcgctct tgctcatcgt cctgaatcaa
    21841 ggtccggaga agagaggtga aacccttggc gtcgggaagc atgtgggcgt ccagggtccc
    21901 aatggcaccg atgatcgcct tggtcagctc ggattcgctc aagtccagct tcttcaaaaa
    21961 gtccgccgcc tggtcgtagg aatccagggt ttttgagagg ttcgggtctc ggtaggagac
    22021 cagggtcagg gaccccgtgt ggcgatcgaa catgcagaag gccccgtagg cccctccctg
    22081 gacccgcacc cgctcccaga gccaggaagt cctgaggtag cccgtcacga cggaaaagga
    22141 gccgtggaag ggaatcccat ccgcatagac gttgaggcct tttcccacgt agttgacctg
    22201 ggagggaacg atcattcctt cgtgtccttc cgggggatcc ggctgccaca cggtctcggg
    22261 ggtggaaccc gtgggcagcc gggaagcgaa ttcgccgacc aggggatcgg tgacggacca
    22321 gccggcttgg tccgtggtga cgttgagaac catggaatcc cgccggacga gaacctgccg
    22381 caaagattcc agctgagcca gaatgccggg ccaatccgtg tccatcctcc gggcaagatc
    22441 gcgcaggaac aacaggtagc ttaccccgtt catctgttcc gaagcccagt ccgcttcgtt
    22501 gaaccgactc cggagtcgaa ggttcaccat ctgatgtccg ttgggaacca tccggtgctc
    22561 ctgcctggcc ttttcctcga gaaccatttg ccgaaaccgt tcccggtcat ccagttttgg
    22621 ccgcgacagg acgtctccca ggatctcgaa taggtccctc agttgacccg acatggcttt
    22681 tccccgcaga aagagccatg tggcggattt accctccctc cgtcttatgg aggagaaaag
    22741 ctcggggcga atcccaccgg tcttgcggct gattcgctgg gtgagggaga cgtagtcctc
    22801 ccggtcggta cccatctcga ggagggcccg ggcaaacaga ggaaccaggg gaacactttc
    22861 ccggggcagg agcttgaggt taaaacccag gtccaggtag acgatcccgc tggtgaaaag
    22921 gtcgtgcagc agcaggggca agccgttttt ctccgccgat tcacagggaa tccttttatt
    22981 ctttttgtcg aggtccgaca ggcggaggct ggggatggtg ctcagagctt cggggggatc
    23041 gggggtctcc tgcagccggc ggagctcagc ggtggcccga atcacctcca ggatctgctc
    23101 gggagcggcc gaccttcgaa aagccgccaa ccgttcctgc tcttcggcct ccattcttcg
    23161 tagcagatcc ggatcgggtc gaaggaccac cgtggttcga tggggatttt ccaggaagtg
    23221 aaggcggatc atctcctcga aaaaccgtgg ggtcgaggca atccgcgact tgagggatgt
    23281 gaggggggct tcgaaggcaa gaagcttcaa cgggtcctcc ccgtagagcc aggtggtcag
    23341 ggatctcagc atccacgaaa gcccgcgggg aaacgccccc gtgttgttct cccggaggcg
    23401 aaactcgatg gtgttgattc cggcttcgat ggtgtccgaa tgaatggttt cccggcaaag
    23461 cctttccagg gtttccagca ccaggggctc cacccggtcc agatcagccg tcgggacacc
    23521 cttgagaccg gtggagaaat acatctggcg aagctcggtc tcgaggccca ccccggccag
    23581 gtcgtccccg agccccgatt cgatgaggct ctttcgaagg ggggacccgg gcatccccag
    23641 cagagcgtaa tgaaggacct ggaacgtcaa gttgagctcg gggtcggcgg tctccgcaag
    23701 cagccaattg accgtcacca tccctttcat ggtgccggat tcctccccat ccccccaaga
    23761 ccccgccgca tacaagtgtt ccacccttcg cgggctcgaa aaccgaggtt gcagatccac
    23821 ccgggaatcc acttcggcct gctcaaagtc acgcaggtac tcgtccagga tccgaaggcg
    23881 ccggtcggga tcgtcgtcgc cgtaaaagaa gattcgggca ttggacgggt gatagtgctt
    23941 ccggtggaac cgacggaact gttcgtaggt cagggagggg atttgagccg ggtctcctcc
    24001 cgagtccagc ccgtaggggg ttgagggaaa cagggactgc tgggtccttt ctgcgagtag
    24061 gttgtccgga gaagaatagg cgcctttcat ctcgttgaag accacccctt tgaaggacaa
    24121 aggagcattg ggaccttcca gctcgtaatg ccagccttcc tgctggaaaa tcggaaggct
    24181 gattcgagga taaaacaccg catcgaggta cacatcgatc aggttgtaga aatcctgcac
    24241 attctggctg gccacgggat agcaggtctt gtccgggtag gtgaaggcat tgaggaaggt
    24301 cttgagcgat cctttgagga gttcgacgaa aggttccttc acgggatact ttcgggaacc
    24361 gcaaaggact gcgtgttcca ggatgtgagc caccccggtg gaatcccccg gcggggttcg
    24421 aaaggtgatt ccaaaaacct tgttctcatc ctcgttggaa agagagagga gctcggcacc
    24481 cgttttctcg tgccggtaaa agcttgccgt cgtgttgatc tccgggattt ccgcttcccg
    24541 ctccagtctg aatccatgaa tcgatttcat cgaatcctcc aatgattctt ccctgggatt
    24601 tcatccgtag taagatgccg gcgacgctca acccctgggt gacgccgggt cgaacgaaac
    24661 cgttgatgaa aagggccgtt catgttaacc ggagttgcct ggtcggacag gccctctttg
    24721 cccgtcgccg ggtgaacggc gggccggatc ccggcacaca ggccctgctg gttccaggtt
    24781 tttatcgggg catgcgcgga tggtcaaccc aaaacaggag cggcgcccgg cgctcccgcc
    24841 cccatgacgg gcgtggcggg ggcggaaaat agctcgagac cgggttagcc cctatggtgc
    24901 cgcttacctc atgttggcgt gctgacgggg aggattttgt gaaaatcact tttttaggtg
    24961 tcggagaagc cttcgacgag gagttgccca atacctcggt cctggtccgg acaacggcga
    25021 gaggggaacc tgcaaggatc ctgttggact gcggcttcac cgttccctcc cctttctgga
    25081 ggaaggcgcc gggaccccag gacctggacc tggtatggat atcccatttt catggggacc
    25141 atttcttcgg acttccggca cttctcctgc ggttttggga aatcgggagg acccggcccc
    25201 tggtcctgtt gggccagacg gggctggggg acgtggttcg aaagaccatg gaactggcct
    25261 atccgcgatt cctgcagaaa ctggcctatc cccttcaatt cgtggaagtc gagccgggac
    25321 gaattttcgc atggaaggat ttggagtttt cgttcgcgga gaacgatcat gggctcaggg
    25381 acctagccgt tcggctggac ctgggaggcc ggtcggtgtt ctacagcggg gacgggcgtg
    25441 ctacggagca aaccctctcc ctggcccacc ggtgcgacct ggtgattcac gaaagctttc
    25501 gatggaaaga cccgacgccg ggacacggga ccgtggcgga gtccatcgag ttcgcgaggc
    25561 gggcccaggc gacccgattg gccctggtgc acctccaacg ggacgagaga agggagaagg
    25621 cccaccgcat ccgggaggtt ctgcagggac tcgagggatt ggaaggaatc cttccccagg
    25681 cgggggacga gatcgtgctc ggggagtctc cccctcggta gagaggtccc ggccgtgagg
    25741 ggatggcggc ggccggcccc gttggattac ccgttggaat tggttcgctt gacggacttc
    25801 ttgtagtctc ccaggtgcca cactttcagc aggtgctcgg ccttctttcg gaacaagacg
    25861 gctgcaccga ggaggatgcc cgcaatggcg atgagaatgt agagccgtcc gtactggtgg
    25921 tttcggatga accctccgct cagagtgagc agaatggtcc cgaaaaggcg ccccacgctg
    25981 ctcaccagga ggaattccat ggaggtgagg tgtcccagtc ccaggatgaa gcacaaatag
    26041 tccttgggaa agcccgggat gaggaagagc agaaagatca ccaccaaccc cttgcggtgc
    26101 agcagataat cgaatcgttc caggaccggc ttgctgacga acttgtccac aaagggccga
    26161 ccgaaagctc gggagaggga aaaagccaca aaggaaccga tcacgagccc gatggtcgat
    26221 aaaatgacgc ccagcgtcgg accgtagagg tagcccccga tgattccggt gacttctccg
    26281 ggaatggggg caatgacgac ctggatggac tggagggcaa tgaagccgag gaaagacaag
    26341 ggacccaagg aatcaaggaa tgccaggagt cgatccttgt gcatgaagaa cttgaagatc
    26401 cccgattcgt gcaggaggta gatgattccc agaatgccga gaaggatgcc cagtcggagc
    26461 cagacggcgt ttcttgttcc gttctttgtc ttctgttcct cggggagggg cagtacttcc
    26521 ggggattgct cgggttggtc cggcacgttt cgtcctctac gatcgttctc tcggcaaacg
    26581 aatcatcctt gcatggtcct ctcgtagcgc cccgggagag agcggcttgt cccgaacgta
    26641 caggaagggt gaagatgtgc agccttcagg gagccaggta caaacagcag aggactataa
    26701 cacgggacac gggagggagc caagggcgga attgcccatc ccgcgcgtcc ttcgcccatt
    26761 ttcgccgggc aacttccgtg ggcccgggag gaaagcagat ccctcgaagg cttccccccc
    26821 gaggggatcg tgttcgatcc tgtcgttggg cgtcctgaac cgcgatttgt cgtcccgcac
    26881 ccgcaatctg caacggggtt gttccgcctg ccgtctcttc cctcaagggg ccagggcata
    26941 cagcaaggct gcggccgatc gaaccccccg gtggaagtcc tcaagaaaaa gccgctcgtt
    27001 gggggaatgg ggcccatcgt cggggcttcc ccatcccatc aagagaatgc tgtcgatttc
    27061 caggacctct ttgagcacat tgacgatggg aatggaccct ccttcccgga tgaatacagg
    27121 ggtttttccg aacccgatct cgatggcccg ggccgccgcc cggattcccg tgcagtcgcg
    27181 ggaaaccagg accggttccg cacgatgaag ctccgttacc gtcactcgga ccccgggagg
    27241 cgtcagggat tccacgtact gtttgaacaa cgcgctgatt accgacgggg actggttggg
    27301 caccagtctc atggacacct tggcccccgc cttcgcggga atgatggtct tggctccttc
    27361 gccctggaag ccgccccaaa tcccgttcac gtccagggtc ggtcgggcgc ttttgcgctc
    27421 caacacggtg tatcccggct cccccggaag ctcgtcgact cccaggtagg ctttaagacc
    27481 gccttcgtcg aaggagagag cggccagttc ctgacgttcc cacgcctcca gggggaccac
    27541 cgattcgtag aacccgggga tggccacggt cccgtccggg ttcttcatct gggcgaggac
    27601 ctgggccagg gcttgggccg ggttggccgt cactcccccg aagctccccg aatgaacgtc
    27661 gatccgagga ccttgaaggt cgatctggaa ataagaaagg cctcgaagtc cgtaggtgat
    27721 ggcagggact ccccgggaaa actgggaccc gtcggaaacc gctacggcgt cagccttcag
    27781 gtccgtgcga tgacgtttga gaaaaccctc cagattggga ctgccgatct cttcctctcc
    27841 ctccaccagg accttcacgt tgatgggaag ccgggacgtg acggccagca cggcttcgat
    27901 ggccttgagg taggtgaaaa actgaccctt gtcgtccgtg gcaccccggg catacacgca
    27961 accgtcccga atgctgggcg aaaagggggg ggtgatccat tcttccatgg gttcgggcgg
    28021 ttgaacgtca tagtgaccgt agataagcag ggtgggcaaa tcgggttgga agggggaccg
    28081 ggccgtcacc accgggtggc ctccggtctc gtgcagttgg gccggcaggc ccaaacgttt
    28141 gagatggtga aggacccact cggcagcctt gcgaacgtcc tgggcatgac gggtgtaagt
    28201 gctcacactg ggaattcgca gaaagcccag caactcttcc acatatcgtt gacgattctt
    28261 atcgatcttc tcgaggattt cctgggggat catcatgggg ttctctcctt ccaaaccaat
    28321 gaaataaggt ccctgagcat tcgcctgggg ccagaataca atttttctgc gggtcggcca
    28381 atacagaaca ccgtctttgg tccccggtct gcggaatccc gaccctcacg gaaggcgggc
    28441 ggaccctccg gcaagccttc ccggcttcgc cggccctcgg ccatgggcga atccgggatg
    28501 gaagcacggt caaagatcaa cacggaggag ccgtgggctc cgtttgggtt ataatgagtg
    28561 cgtggtccaa gggacccgca tggggcaaga gtagcgaacc ttctcggaac ataaggggtg
    28621 gaggggtccc aagaggcggt catccggcaa cgaacggccg tctaccgacc gatcgtcgaa
    28681 agccgtcaac tttaacttac ttccgggtcc cggtttgtgc cgaaagggcg cagaccggtt
    28741 gcggacgggt gggagacatg gtctttgaga gcttttcccg ggcggaacag gtggacaaag
    28801 agctttggaa ccggttggcg cagcacgccg ctccgaccat ggaatgggaa tacttccggg
    28861 ccctcgagga atcgggggcc gtttcccggg acaaagggta ccgacccttt catcttgtgg
    28921 gatatgaagg cggcgaaccc gtactgctgg ctccgtttta cgaaagggac cgggcctggg
    28981 tggaattcgg ggacgggggc ctgatcgaat tcctcaccga attgacggga atgcctttca
    29041 acgtggggct ggtcggcacc attcccttca cccccgttcc cggctaccga tttctgcatc
    29101 gttccggcga ggaacccgta gccttgtacc gggccctgct ccactacctg gatcacctgt
    29161 gcgagaccaa gggttttgcc acctgccgta tctactttac ggcagccgat gcggtgcgcc
    29221 ttcgagaagt cctgaaaggt cacggctacg tagggctgaa aaacgaacac tgccgatggt
    29281 tcaatccggg tttcaccagc ttcgaggaat acctgggaac gttccggtcg gccaggagga
    29341 ccaagatcaa gcgcgagctc cggaccgtcc gggaccgagg ggttgcggtg cgcatgctgg
    29401 caggagagga cgtccccgcg tcttatttcg gccgcatgta ccagctgtat cgtcgaacct
    29461 gggagaagca tatggggacc ctcattgcgc ctttcctcaa cgaggatttc ttccggttgc
    29521 tcgatgagaa tttccggcat cgatgcgcct tttccacggc atttcgagag gaagaggtgg
    29581 tggccatggc catcttctat gccaaggaga gaaagttgta cgggcggtac tgggggacct
    29641 tcgaggaaat ccccttcctg catttcgcga cgtgctatta ccatcccctc gactacgcca
    29701 ttcggcaggg aatggagatg atggacccgg ggtttggggg cgaacacaag ctcttccgcg
    29761 gatatgaaat cgtgcccgtg tatcacttca ttaagttcta cggggaggaa ccacgcaggg
    29821 ttgccggggc ggttctcaag cagatgagcg atcgtggccg cccggtgggg ttccgataaa
    29881 cagtggagag gtatccgaat acaagagagg aaaagaaaaa aagagagagc cgggtaggag
    29941 gggggaaccc ggctctctca gggaggagga aagatgttct tcccgtcttt atcgtaacac
    30001 ccccgtcaag aggggtaatc ccccggggag aagcacccgg gaaaaacacg acgggcggac
    30061 ccggcctgcc cgcggtgcgc ccaggggcgg atccggggaa agattgcagc cgatggtgtc
    30121 cggaaaggat gcgttgcccg ttcaaaaggg gggaagatca gttcccttct cggctgcaaa
    30181 cgggccggga cggcgcactc taggggccct tcttcttgtc tttgatcaga gcgatggcct
    30241 tgagggcttc caggtacagg gtggcatcct ccgccgttag catcacccat cggccgggag
    30301 gctgcattct gccgtccttg aaaatggttc ccacaaaatc accggagctc ttgtagaggt
    30361 tgaacccatt gtcctggtac ggttgaagtg ttcccacgca atccctgcct tggtaacaga
    30421 tgtcccatgc ccccccgcct ttggaccgga cctccagttc gtccgcccgg gcaagggcgc
    30481 agaaagacag caagagagtc gccgcaaggg caagtctgaa agccgatttc atcctgacct
    30541 cctatggatg gggaagggtg tgtgacgcac tagcgcttga atgaggacaa tcctcgagac
    30601 tatagcataa cacgaaggat ccgggggctc tatcccggga ttcgaactgg acttgatgca
    30661 agatcatcga taaggtacac aagcccggtg ccgtgaacgc gccggaccgg cttcacccgt
    30721 tcgtgccgtt ttcacggcgg gttcgaattg aaagcctatg gacaggcagt tccggaaagg
    30781 aggaggacgt gacgacgcag ttgaccgatg gccaaaaata cctgctcccc aacggggaag
    30841 tcgtgattgc gagacatctt gcggatggga tcctgctgga gtttaagagg aaatatcgcg
    30901 tgcccctcga ggtgggggag acgggagcgc tcatcctccg gggaaacgag acggcctgga
    30961 gggtggagca attgacccct gtgaaggacg attgaaacgg accgcgggac cttgcgaagg
    31021 cggtgagcgc caagaggcgc cctctcccga cctcctcgcc gggagagggc gaatcatgct
    31081 gcggaatttg gggagaaatc gccgatcttc cccgccccta cgattcccgg taggttccgg
    31141 gcggaatgca ggcgccgtac cgggatttgg ctccctggag cttggcgatg gcggctttcc
    31201 aaatggagta gaccttctcg gggcaatccg gggccgattt ctcaaagaag gcaatggccc
    31261 ggttgagctt ggcaatccag ggatcgattc gaaacttgaa gagctcgtcg tagagctccc
    31321 gggagaaatc ctcttccaaa aattcccgaa acatcccggc caggtcctca tagatgggaa
    31381 tccagcccag gggcgtccgg tgagccccaa actctccgtg gactctgcgc tccgcccaat
    31441 ggatccacac cttttttgcc atcttgctgg tgaggaactg ccctcgggaa ttcttcagaa
    31501 agtagttcac cgtgtagatc ctggggacgt tctccattcc ccggacgaag tggatgttgt
    31561 tgaggacgta ttggccgatg ggatagctga tgaagtccag gtttgccatg ggctgcgggg
    31621 ccaggactcc ttcttttccg atggtggccg acgtggtttc cgattccaga gtgcacgcct
    31681 tcagaatgat tccgctttcc cagtcggggg attcttcgat ggggacggag gtgtcgctgt
    31741 cgcggcctcc gtaaatgatc ccccgcaccc acaccccgtt cttggccttg tagttgtggt
    31801 cgatattctc caggtccatc aggcggagtg tatagcgggc gttgctgtga gcgaagggga
    31861 tttcgtttcc caactcgtcc ttcttgccac ggtaccagtc tcccgagaag ttcgtcccct
    31921 tttcgggcac ctccgttccc attcccagcc agtaaggagc gttgtcgggg cccaccagga
    31981 cgttggagta gatgatttcg ttgccggggg tctggagggt cttgaaaatg atggggtcgt
    32041 cttcgggatt cacgtcctta atgatcccga atatgccgcg ctcgacgttc acggctcgga
    32101 attccccccc gatatttcgg aaataggcga tgtcgtctcc cacgatcttc tcccccggga
    32161 gcatggcggt ggaggtcttt ccgcaggcgg aaggaaaggc cccgcaaaaa taggtggacc
    32221 ggccgtcggg gggattttga accgccataa tgaacatgtg ttcgcaaagc cagccttcct
    32281 ggccggatag ccggatggcg aggcgcatgg cgtgtttctt gagccctacg ctgtttcccg
    32341 catactggtt gttggccgaa aggaccagca tgtcttccag gtcctggtag atccggcgct
    32401 tgtcgagatt gacggagttt ccccgatcat ccagttcccc ggcgctgtgg atgaaccgga
    32461 agaacctgtc cttgtcttcc attcccatga agtgctcgta gccccggcga tacaaaatct
    32521 cttcggaatg gcacacatag aaggaatcgc tgatctgggc gcacccgatg gagaaaggac
    32581 tcaacgtcgg cccttcgcag ctgagcttca cgacggcctc cttgcccttc atgattcccc
    32641 ggaggatttc ccgtatctcg gccatcccct cgtcccggtc cttggcggcc agggccttca
    32701 tgggggcaag gagttccctg cggaccaggt tcttggtggc gccgggtgcc ctgccctggt
    32761 cgtgatatcc gtcaaagtgg acggtgtggc cctcttttcc cagcttcttt tcctccccca
    32821 gctccaggga cctcttgcgc aggtattccg cgtcctcgtc gctgtcgtcg cacatataga
    32881 ccgactcggg ctcacacaac tccacgtatt cgcctacgaa gttgaataac ttctcgttgt
    32941 tcaaaaccgc cagcttgtga aaactttccg cggacatctt gccctgcaac agatcgaaat
    33001 accggttcct cgtgagatca accatcgctt ctgctccctt ctgactctcc atctacccgg
    33061 caaagccggc gaatcgtgcg gcgttcgccc gagaccgatc cggacggacg atgcggcgtc
    33121 attgtgtgtg ccgatcaaga gaaatctgta acaattccaa ggcacaactg tcaaggactg
    33181 tgtcccccgg caagcccctt tgcggggacc cccccctgtg aagacgatgt cgcccgtttc
    33241 caggcctgcc cggagagcac acggtcctgt gggagggtcc tcatccctgt cggccttttc
    33301 caaggaccat ccccaaagga gtgtacccac cccatggctt cccttcaaac aacacgccgc
    33361 aggggacgtc ccttccttgt gcaaactccg ttccaggata ggggagttca aaaagagact
    33421 ttcccggtga gttgggttgg agcggctggg ggacctttct attcaaggac cagttcggtg
    33481 tcttgggtca aggcaaaaca ttccggggcc gtacccttct tgtcggcgat ctcccggcaa
    33541 tgcctcacca ggcgatccat gtcctcatcc ttccggtcct ggttgtggtg gaagagaccg
    33601 aggcgcttca caccggccgc catggcgaga tccagggcgt ccaggtacgt ggaatggccc
    33661 caggttctcg tgaaggcgta ttcttgggga gtgaattccg cgtcgtggat gagaagatcg
    33721 gagcctcggg caaattccac atattcctcg aagctccttc cgtttttgtg ccggtatccc
    33781 agttcgttgt cggtgaggaa cacgaactgc gtgtccttct ccacaaactt gaagcccagt
    33841 cccacgttgg ggtggctcag gtggatgggg tggatctgaa tcgagtcgat ggtgaggatg
    33901 gaggggcagc ttccatcgta gaggatccgg gcgttgagct tgtcgaaagg cacgggaaaa
    33961 agcggcgtgc tcatggcctt ggacaggagc cgctggatgt ttccctgggc cgtgggacat
    34021 ccacggaggt gaaggatggt cttgtcgctg tagatgggct tgaagaaagg gaaccccagg
    34081 atgtgatccc agtgagaatg ggtgaagagg atgttgaact cggatcttcc ttcgaggatc
    34141 agcttcttgc ccagccgtcg cactccggtg cccgtgtcca cgataatgat ttcatcgttc
    34201 ctgctgcgga cttccaggca gcacgtgtca cccccatacc gaaagtattc cttgccggac
    34261 acgggaatcg agcccctggc gccgtaacat cgaacaatca tggagtcctc cacccacaag
    34321 agcactgccc ttgggactca cagaggggaa cgaaagggtc cggggccgtc ggtggtggcg
    34381 tcattggtgg cccaccattc ctttctacca taattgaggc tacagattca tccattctct
    34441 cacccgtcgc aggccgcaat ccgatttcct cgaaggctct ccatctcgtt ggatcaaagc
    34501 agcgtgcgtg cgtcccattc taggcaaaaa gccgccgagt cacttgatcg ggattcgaac
    34561 gaaacccccg aacccttttg ggtccgaggg ttgtgcgaga tcgttttgct ttcctaacag
    34621 gggcctttgc gcctattctt gtccgcaagg ggtgcagttg ccccattcgt cgatgcactc
    34681 ttcacaaaat gtccgacggc acatgcggca ttcggatacg gcggcatccc gggtgtattc
    34741 ctccccgcat gcggcacagg tgaacatctt ttcttcggtc gaatgctgtg ccatggtgtg
    34801 ctcctttcct gtggttcggt tcttcgctta ttggaaacta ttggcggaaa cggccgtgtc
    34861 aagggttttc ggaaaggctg tcgagtgtct acgatccatg gcgccggatg ccggagttga
    34921 gggcttcgtt cccggtcccc gcggggatcc tgtgggattc gtccgtgggg ggctcaggtt
    34981 cctagcagtt caaggaccgt ttgagccact ccttgagagg ttcactgacg gcgtagacct
    35041 cgacgccggg cttgcggccc ccttcggcga cagcctcgag aatgagctcc tcgggagctt
    35101 ccacccgggc gatttccacc atttgggaga cgtcccgcct caccagggtc acgcacaagg
    35161 gcctctccca tccgtaaaca agaaaatagt agcttgcctg gcccgggtat cgtacgaaat
    35221 cgtctttgcg agcccaaccc gtgccccatt ccaggtaagt ttccaccgcc ttttccggag
    35281 tcatatccca atcgatttga tcaatgattt cccggtgtcg ttgcagttcc tccaggttcc
    35341 acacggcagt ccctccgtta tttgggggtc cgcgggaccg ggtttgaggc cctcagcccg
    35401 taccggtccc acgagtggtt tcatccggca gaccgaaatc atccatcggc acagacaagg
    35461 gtaagtctcc caagacttcc ccgcaatgca aaagcatcca acctttcgaa aactccggaa
    35521 gccccgcctg tttcgaacct cggtccaggg aatgctctcc ggaattgttt caagttcttg
    35581 cttccatgtg acgatgactg gctcaaaggt aatatgcaca gacgttggga gacataagga
    35641 acacgctcac aaggatgcgg cgatgtgccg ggaccggcga ccggatgcac aagaaggtgc
    35701 tttccgcgag gggatcggcc ggtgccggga agaggggttc ccgggcggcc gggcacctgg
    35761 gcttgaggac gaatgtcccg aaaggcggtc ttcccctcgt gaatgatttc gcggcagctg
    35821 caatgccttt ggaaatcggg ggctgtttcg aaaggaggta ttcatgagaa tcaggctggg
    35881 gcttcaggca aaattaatcc tgggcggtat cgggtttatg ctgactccaa tgctcattgt
    35941 cagctttttc tcaacgatca aggcttcgag tgccatggag tccatttcca gaaacggcgc
    36001 ggccaaccag gcccaaagta ccgcggacat gatccagcga atcctcctcg aggaaatcaa
    36061 gcttgcgacg gacctttccg tgggaaacac caccatcgac gtcgggacca aaatggccag
    36121 agaaggacat gatggatctt cccaagaggt cgaaaagctg agccggaagc tcaccgaggc
    36181 gatgaaacgg attggaaagg attatgaaac gatccttgtg gccgaccaga acggcctcgt
    36241 ctatgcggac ggctgcggcg gggcttccgt ggggactacc gtccagagca cggattactt
    36301 tggaggagtc agatcgggaa aggtaaccct cggttccgtc cagccgtcca tagtgaccaa
    36361 ggaacccgtt cttcccatcg gcgctccggt cttctccaag gagggagatt tcgtcggctg
    36421 cctggtggtg gtcctgaaat ccgacttcct gaacggtcaa attgcggaca cgcgggtcgg
    36481 gcagacgggc tacgtgttcc tggtcgacaa gggcggccgg atcattgccc accccaaaaa
    36541 ggacctcatc ctgaaggtca acatttcgtc ggtcaagggc atggaaacaa tcgccgaaaa
    36601 aatgaggcag ggacattcgg gggtggagga gtactccgtt gaggggatcc ggagcctcgc
    36661 aggctttgct cccgtgaaac tgacggggtg gagcgttgcc gcgacccagc ccctgagcga
    36721 agggatgact ttggtgcaat ccgttcgcaa cagcatcctg gtcatggccg gcatcttcct
    36781 cctcgcagcc ttcctggtca tggtgttcgg ggcgagggaa acggccaaac cgatccggtt
    36841 cgccgccgag ggtttggggg aaggcgccga gcaagtggct cgcgcctcga tggaggtttc
    36901 ccaggccagc cagcaggtgg cccggggaac actgcaacag gcggcgtccc tcgaggaaac
    36961 ctcctcctcc ctcaagcaga tgtcttccat gaccgctcga aacgcggaaa acgccgcccg
    37021 cgccaatgct tacatggaag aggccatgac cacggtggat gaggccagtc ggtccatggc
    37081 ggggttgacg gcctccatgc aggagatatc cggggctagt gaggagattg ggaatatcat
    37141 caagaccatc gacgagattt cctttcagac caacctgctt gccctcaatg cagcggtgga
    37201 ggcggcgagg gcaggggaag ccggggcagg gttcgccgtg gtggcggacg aagtgagaag
    37261 attggccgtt cgagcggcgg atgccgcagg gaatacctcg atcctcatcg aaaaaacagt
    37321 gaggaaggtc cgggaaggat tcggactggt caaccagacc aataccgagt ttcgtcgggt
    37381 tgcggactcc gtggcaaaat ccggaagcct gatcacggag atcgccgcgg cctgtcggga
    37441 gcaatctcag gggattttgc agatcaacca agccgtctcc gacatggacc aggtcgtccg
    37501 gtcgaacgcg gccggtgccg agcagtccgc ggcagcggcg gaacagatga atgcccaggc
    37561 ccgacagatg gaggaattcg tcggaggatt ggttgcgttg gtcaagggcg aaagaggcaa
    37621 tggggacagg ggagaaagga cagccttgta cgcctgcgag gggagtttcc ccgggctgga
    37681 tccgggaagc ccggggtctc ggatttcggc agggaggctg tcgagtgccg acggcaactc
    37741 gagagggacc ctgcccggcg gtgatcctcg atccctggcg gatcggtcgg gctgtcttcc
    37801 cggaaaccgg gactaggaca accccaggct cgggcgacag tggtcttttg cgacccctgc
    37861 cgggtttcgt cctctttctt cccgagacgg ttcgttcccg cggagcaggg gaccggggtt
    37921 tcctttcagc ctttttccca tccttcttcc aaggcggaag aatcggtgga cggccttctt
    37981 ggccgggaac ggctctgacg gtcccggtcg ccgatttttc cagttgtctg catcaagttc
    38041 tgcagattgg atcggagcat acggaacctg tttttgccga tgtgcatcgg ccatcgtctt
    38101 gacccgcgga agggccgccg agaatgactc tccccatatc ccgacggcca tggaaagtat
    38161 gcccagatcg accgttgcac ggagatcccc gcttgtgaaa ggaacaagtg cagctatcga
    38221 gacgacgggt actagagatc ggtcgccgtc tgccggggcc cgacgagggg ccccggtgat
    38281 ttcccctgcg ccgggtccgg gcaggggtcg aatggaagga aagtggaaga cggaaaggac
    38341 ctgaaggagg agcaaggacc tcgcgcgttg agacccgaca gtatcgatcg cgcaagacga
    38401 gggcggaaga cggggagaat ttccgggagc ccggtggaac gcgagatcat tcggggggct
    38461 gccagaagca aacatccccg gcaatttcca ggagctcggg gaagatgtcc atgagggcca
    38521 ctaccccgac gattctgccc tcgcggacca ccatgggttg acggatgttt tccttgaaca
    38581 tcatgtaaat ggcccggttg atggtatcgt cctccttgag gaggggatgg aggggagaca
    38641 tgacgtctct caccttcttc cgggattgct tgtgaaccag ctcaaaaaaa tcggtttcca
    38701 gccattgcat tcgttcctgg acttcggcga tctcttccgc cgcctcggct gctttgacgg
    38761 agatcgtccg ctgaagggtt tttgaaatgt cggccttctt ggccgattcc ggcacaagtc
    38821 cccgaatcag gtcgtacatg gagagctttc ccacaatctc ccccgcagca ttcttcacga
    38881 aaagcgtctt atggtacctg cccggctcgc ctgcctggat cttgtcatag ttgtggcgaa
    38941 tgatcttgag agcatcgcag agtctgtcgt ccgccccgac catgtcgtag tcttcgatgg
    39001 gggacatgat ctctcttacc gtcttgtttc ccgtcatgtt gagacctcct gtctgttgat
    39061 aatgggcgaa ataggggcac ggattttcgc cgagtcttgc ggatactcgt cactagtagc
    39121 atagcccccg cccgtcttca actgccggca tttccttcac gggcatccgc cttcggacgg
    39181 tttcgaggtt tccggctgaa gacctccggg aatgggggat tgtgctcatg cggatctttt
    39241 tgcaaacgat aggcctttca ttgtatgctg cccgtgtacc ttcatggtct cccgcctcgg
    39301 cagcggccgg acaaccaaag actcgacggg ccgtcccggg ggttaggagc gtcaaccttg
    39361 tggaatgacc cctctcatgc gccgtgacgg tggttgcgca ggaaatcggt cctcgtcttc
    39421 ccaaaacgcc aaccaatcga ggaaaagggg atgccatggt tctggggaga agtctgcgga
    39481 tgatgttgtt gttctcttcg gtgctggttc ctccgtgtcc ggccaaacaa gggaaggctg
    39541 ggaacccccg ggactcggcc gcgatccggg gagtggtgga agggcttcac aagtcattca
    39601 acgcccagga cctcaagggg gtgatgatgg ctttttcgtc cgacccgcat atcgtcctca
    39661 tgggcaccgg ccccggagaa gtgtaggtgg gagacgaagc cgtgagggga gccttcaacc
    39721 aattcttcaa ccggttcgag gccaatgctt tgaacttccg atacgactgg gttgttttcg
    39781 cttcccgggg cggttccggc tggtttgcag ggaccaccac gatggaagcc gtggtgaagg
    39841 gggagaaaaa ggaacgaagt ttcaacatgt ccgggaacct ccggaaggaa aagggcaaat
    39901 ggcggattgt cgccgtgcac atttcccggc tgcgcgccga agagcaaccg gctgcaggga
    39961 tgggttccgc ctcgggcacc ggggccgggg ggacccggtt gctcggggcc gcttgaagtc
    40021 aagcgattga gatcatgaat gataccctgg tccaaaacgt tccggtcgag agaatcgaag
    40081 cctaccgagg acgcagaatc atcgtccgat ggcaggattc ggagaggatc gatccgggcc
    40141 tgaccgacaa cccggaaagc atcgcctacc tccaggtgct ggggacgggg ggaaacctcg
    40201 atgagctgtt tcgctgggag cagccggtac cggtggacct ggtcctcgag gacctcgaga
    40261 aggaacttcc gcttctctac cggtattccc ccctatcggc ccgtcacccg gtccgggttt
    40321 tcattccggt gacgtcgggg ttttctagag cagtcaagct ggctgcgtcc ctggattttg
    40381 cggtcaagct cgagccttcc cagccggacc cccaacggat gggagagctt ttccgggtcg
    40441 tcgacttcta cctgcaccag tggacggtct ctcagccggt ggagttcatc cactccatgt
    40501 ttccggggtt ctctcacgga tcgcccttta ccctttgggc ggtgcaggaa gaagacccgt
    40561 cgcggtttcg attcgtaacc cgggaagggg aagaaaccgt ctcccggaaa tttgccgggg
    40621 tcgaaccggg acacgagctt ccttctttca tagccgggtt cagccgggaa ctcctcgagg
    40681 atgccgggga atgttgtgac tgccggtcct tcgaagtctg cctcgggtat ttcaaatggc
    40741 cggaaaagga ctatcgatac gacggggtga aaagaccctt cgatcggctg gagaaaacgg
    40801 ccgaggactg aagggggatc tttcctggtt tgttccgctc cggggagagg ggggaatgat
    40861 gacgcggggg cccttgtccc tcatcctgct cccgaccctc ctgtgcgacg ccgattgtga
    40921 attctgcatc cagaagaaga ccgatgggca gctcacccac gacgaactct cggtcgtggt
    40981 ccgcaaagtg gtggaccaca tggtgcggga gcacatcgaa actctttccg tctaccggca
    41041 agggggcgaa gtcatgaccc cggctccgga atggttcgaa gaggctcacg ggatcatcgg
    41101 ggaggccgtg aattccggcc gtcgtcgggt cgagcacttc gtccaatcca acctgatcgc
    41161 ctacagcgac cgccgggatt gggtccttgc ccaaatgttc ggcaacagcg tgggaacctc
    41221 catggactat cccggtctcc atcggaagct caagaggggg gtccgaagga atacgaccga
    41281 atatggtcgc ggaatgttcg agcggcaaga gatgccggca tcgaagtcgg ggtgatcccc
    41341 attcccaatg aacggaccct cgagttgggt gccgaacggt cccattctca cttcacggac
    41401 gagttgggaa tccaggattt tcaggtgaat accccgtttc ccggaggggc ctccaatgcg
    41461 gtcaaaccgg gttatcccct ggagaccggg cggttgagcg aattcctggt ggctttggct
    41521 cgggtttggc tcgaacgggg tttcgaacgc ggggtgcgtg tcggtccctt tgacgggctg
    41581 gtgcgctatt tcgtccatgg cggcaaggac ttcctgtgca tatggcggga caattgtgcc
    41641 aacgagttcg tctgcattga cccccggggc catgtggctc aatgcgactg ctgggcgacc
    41701 agctacccgg agtcacggtc cggcaacgtc tttgattccg gaagcctgtc ggacttgctt
    41761 cgaaacagtc cggcccgcct gaggattcaa tcgagacccg gagtcctgat ccgaacggaa
    41821 gactgcctgg actgcaattc cctgagcatt cgccatggag gatgtccggt aagggcctac
    41881 agcggatacg gggacctttt tcggaaagat ccctactgcg cgcttcacgg gcggatcttc
    41941 cccgccgtgg aggaactggc ggcgcgatac gtgagctgag ggtggaaggg gagtgcacga
    42001 ggatgccgag tcgcaaacgg ggtagggctc tggactgcct cgatggaggg tgcaatgact
    42061 gcggaacggc ccagtgggaa caccggtggc aacgaggaac cccttgagcg catccggggg
    42121 atgctgaaaa gcaagtcggc cgaaggggta ggtcgagggg gtgcaacgga ggggagggtc
    42181 ccgccgtacg gcgatctgac cttgttgaat acctctcggc tgatcctgga ttcggttggg
    42241 agctcctttc tcgaggatat tttccgggac tttacggagc tcttgggtac cacctgcgcg
    42301 gtctatgaga aaaacggcga ttacgccctg aggatgttcc cgcccggttg gtgtcggttc
    42361 atggatcagg catccaggga tggttgcctc acggcggata accgggaagc cctcgattcc
    42421 ggccaatggc actgtcatga gtcctgctgg aaggaagcgg ctctcctttc gatccgagac
    42481 aaagggcccg tagacgtcgc ctgtcgcggg ggaatccggt tgtttgccgc cccgattctc
    42541 gcgggtgggg aagccgtcgg cgccgtcagc atgggctacg gagatccacc ccgcgacgag
    42601 gcaaggctga acgaattggc caatgcctac ggtgtccgga aagaagagct ccgccgacac
    42661 gccgacgctt accctacgcg ccccccgttc atcctggatt gggccgggaa gcaccttgtc
    42721 tccgtggcgc gatggattgg tgagatcgtg gagcgaaggc gggcggagca agccctgcac
    42781 aggagcgagg aggaattccg agccttgctg gaccatgcca aggacgcggt tttgatccat
    42841 gacgcccaag gccgcatccg ggaggcaaac ctggcagcct gcgagcgcct ggggtaccgt
    42901 cgcgaggaat ttttgaaaat gacccttctt gacctggtgg atcccgagca aaccgcccag
    42961 gtcccccagc ggttcaatac gctgcgaaaa cagggtcatc acttcttcga gagcgttcac
    43021 cgaaggcgcg acggcacggc ggtgccggtg gaagtgtccg tgcggcgtct tgaacgggcg
    43081 cgggaagagc tgttcctggc cgtctgttcc gacctcacgg agagaaagcg ggcggaagcc
    43141 gccctggccg aggtcgagaa gcgtttctcg gtgttcatgg aacacctgcc tgcgggagtc
    43201 ttcatcaagg acccgtcggg gcgtctcctg tttgccaacc ggtacctcaa ggagcttttc
    43261 gactggggcg attgcgttgg gaaaaccacc tgggagcttg tgccccggga actggccgag
    43321 aggatggttg ccgatgaccg aaaagccctg aacgagggtc ccctggtcat ccaggagacg
    43381 gtcaccgacc cccgcggcac cgaacacttc ttcgaaacct acaaattccc ggtcgaggtt
    43441 gtcggctccc cccctctgct gggaggcatc acggtggaca tcagcaagcg caagcgggcc
    43501 gaagatgcgc tcgagaagcg catactggcg ttgacccaac ccctggatga tgccggaaac
    43561 atttcgttcg aagacctgtt caacctggcc gatctccagc acctccagga tctgtttgcg
    43621 gaagtctggg gggttgccgc cctcatcacg cgaccggacg gcaccccgat cacgcgaccg
    43681 agcaacttca ccgatttctg cagcaagttc atccgcagga gtgagaaagg cgtccataac
    43741 tgtcaggtat ccgatgcgac cctgggacgg cataatccct cgggaccgat catccagaag
    43801 tgcttgagtg ccggcttgtg gggcgccgga gccagtatca ccgttggcgg gcgccacatc
    43861 gccagctggc tcatcggcca ggtccgcaac gaggctcaaa gcgaggcgca gattttggaa
    43921 tacgcccgcc tcatcggggt cgacgaagga gcgtttcggg aggccttcct cagggtcccc
    43981 atcatgtccg agaggacctt cgaacaaatc gcccactcgc ttttcgccct ggccaatcag
    44041 ctctccaata ccgccttcca gaacatccaa caggcccggt tcatcgccca actcaagcgg
    44101 gccgaggaaa tcctgcggga gagcgaggcg aaattccggg gcatttgtga agaatcgccc
    44161 atcggcatcg agctctacga ccggcaaggc acgctgctcc acgccaaccg tgccgcttac
    44221 gacatctttg gcgtttccga tccctcgacg gtgaaaaagt tcaacctttt cggagatccc
    44281 aacctgagcg aagaactcaa ggccagactg cggcgagggg aaaatgcacg catcgagatt
    44341 ccgttcgact tcgagcgagt gagggccctc gggttgtacg agaccgccaa gagtggggtc
    44401 gtccatctgg acgtcttggt cacccccttg aaagaggtcg cccagggacc cgtcatcggg
    44461 tacctggtgc atatccgaga catcaccgag cgcaaacggg ccgaagaagc cctccgggat
    44521 tcggaagcaa ggtacgcggt ggtagtggaa caagcgaagg acggagtggt gattatccag
    44581 gacgacgttc tccaattcgt caatcgttcc gtgggagaca tcctgggata tgcccaggga
    44641 gaaatgatcc acacgccttt ctcggattat gtggccccgg agtatcgccc aatgatcgcc
    44701 cagcgggtca gggatcgact ggaaggcaag gaggtcccct cggtctacga agccgcactg
    44761 gttcgcaaag acggcacaag gatcgaggtg gagctctcgg caaacatcat tcaatacggc
    44821 ggaaagaccg cggacctggg cctcattcgc gatatcaccg agcgaaagcg aatggaggag
    44881 gagcgccgca ggttcgaggc acagatgcgt gaagtccaga agctcgagag cctgggcgtg
    44941 cttgcgggag ggatcgccca cgatttcaac aatctcctca tggccatcct gggaaacgcc
    45001 gacctggccc ttgcctcgat ttctcccacg tcccgcgccc atcaccacgt ggaggagatc
    45061 gcccgggctt cccttcgggc cgcggacctc tgtcggcaga tgctggccta ttccgggaaa
    45121 ggacgcttca tggtcggctt ctacgacctc tcggaaatcg tccgggagat ggcacaaatg
    45181 ctcgatgttt ccatctccaa gaaagcgagg acccgtttct cgctcacccc ggatcttccg
    45241 gcggtggaag cggacgcaac ccagttacgc caggtggtca tgaacctcat caccaacgct
    45301 tcggatgcac tgggagaaga aggaggggtc atttccatcg cgacgggtgt caaggagtgc
    45361 catgaggcgt acctttccga gggttacctg gacgacggcc tacccggggg aacctacgtc
    45421 tgccttgaag tatcggatac gggatgcggg atggacgccg aaaccctaag caaggtgttc
    45481 gatccattct ttaccacgaa gtttgtgggt cggggactgg gcttggctgc ggtgctgggc
    45541 attgtccgcg gccacaaagg agccgtcaag gtccgcagtg aggtcgggga aggaactacc
    45601 ttcgagatcc tcttgcctgc cgtggcatgg gagtcgggcg ggaaggcggg agtcgcggga
    45661 ccaggcatgt cgctgcaggg gggaatgcag ggcgggggca ccatcctcct ggtggacgac
    45721 gatccccggg tacgaagcgt ggcgtcggag atgttggtcc aactgggctt ccaagtcctg
    45781 ggcgcggaaa acggtcgaga ggccctggat gtcttccgaa cccgcagggc cgagatcgcc
    45841 tgtgtgatcc tggacctttc catgccggtg atgggcggcg aggaggcttt ccgggaactg
    45901 cgcaacgtga gcggcgacgt gcccgtcctc gtttcgagcg gctacacgga acaggacgtg
    45961 gcgcaacgtt ttgcgggcag aaaacccacg gggtttatcc agaaaccata caccatagcc
    46021 aatctgcgcg gggccctgga ggaggcgctc cgcccacccg aggatccccg gcctgcctga
    46081 cgctgacggg catagtgaac agcctttgga gttccgggga ctcgtgaaaa ccgtgctccc
    46141 aaaccgcaac gcgctgcggc aaacggttgg gatcacacgc cgggccaaac cctgtggtgg
    46201 tgtcgggcgg tcgctctctt aaggcctggt taaggtcgag cctcgtggcg gccgatcctt
    46261 agatcctgct gctgagctcc gggtcccagg gtctcgtctc ggtttgcagc cgtgcccgca
    46321 ccccggcgca actcgacgtc gtagtaggat ttgaagacct cccggatcga ccgggtgtcc
    46381 ggcagcacat agacaatgcc gcttgtcccg gtaaaggccg gaagcacgat gctgtcaaag
    46441 aagttggccg gaacattgat acagccgtaa gagatccggt tgtcggtggc cgttggggtg
    46501 gccagtcgct ccaggcggcg ctccgcgggc tttgtcgtga ccacccgatg caggcccacg
    46561 gaagactcgt aatccaccca aagcatttcc ttgcccttgg gatcgtaacc catcgcggcc
    46621 acgaaacggc ctgcaggggt cgttcgttcc tcgggacgaa cctcggacaa cttccgttgc
    46681 ccgattccgg ggatggattc atccccccgc gccaggccca gcaaaaccgg ggctgcgccg
    46741 agaagtcgcc cgtccgcatc aaaaacgaag accttggcgt ttaccttgtc gatgatcgcg
    46801 aacgtcaatc ctagattgtc tccggagtca acgacccagt ccgccacctg ctgagtgtct
    46861 cgagacttcg gctcctgacc gaaactggcc cgtttgggat gggatgcgac ctcatggtgg
    46921 gcctcataag cggagacggc tgtcggcact tcggaaacgg atacggcaag gctcgggttg
    46981 gacggttgcg ccgcggtctc caccgccgca accgggtggg ggatcagacc gacggccagc
    47041 aggcccaggc aaagtccccg ggtcatgacg gccgtgaggg cgcgcacgct cccccggtcc
    47101 cccgcgccgc gcaactttcc ccaaaggcgc agccgtgtcg gctttgtcac cgcaacgtaa
    47161 aataatcctt gccgtgtcgg catgttcttc ttttccgagg ataaccgaaa ggcgccctgt
    47221 ctcgtgatca tcgttccacg agacagggca aggtgggagg cctagttacg agcaggcttc
    47281 ggcggaggtg gtggtggagg cgccggagcc ggaggtgcgg gtttgactac cgcaggttgt
    47341 ggaggcggct cctccaccgg ttgcttccgg acacacgagc aaccagccaa agagaaactg
    47401 atgcagatga tgaataatgc aaaccaaaat gatcttttca taacccaccc tttctcagaa
    47461 tccgttgttg ttagtcttca ctcttttccc attgaacctt cctaacgacc ggttcaacac
    47521 cccctgccga aatgaaaccc gtccaccctc aaaaccttca ctgaagaccg tacatgtatc
    47581 ctgaatatag tcaagttcat tttaacggtc ggatggcccg gggccttcat tccacaacta
    47641 gcccctcgac cgtgagggtc ccgctcgggg cctccgtccc ccgggccgga cccgcctgaa
    47701 tccttgcccg aaggcaaagt acggcaaatc cgaatgcaca accgcccaaa gacttcaaga
    47761 agccggggcc gcgatggcgt cgtgatcgaa cggaccgact gcacgccttg atttcggggt
    47821 tcttgccggg tttcggcaag aggagcctcc ccaaccgaaa tataggccga agatcgagtt
    47881 tccgggtaca agaagggatc cccattccgc gatctaatac aaggatttga tgtcggcctc
    47941 ctcttcctcc tcgggaaccc cctcttcttg cctcgatgtt gctcggctga ctacttggac
    48001 cggagcgggg tcgtccacat ccaactcggt ttcgttgacg atttctcccg gcgttccaac
    48061 ctttgcccaa ttgtacacaa aaacggcgtt ctgatagatc agccggacgc atccgtgaga
    48121 agcggcatac gttggcagaa tgccgccatg aagaaagtaa ttcccgaaca gtctcaacga
    48181 atacggcatc catgcattgt cgtacttggt cgagtaatgg tctttctcct tggacaatat
    48241 cacgaacttc ctggccgggg ttcctccgtt ctttccggat gagatgggaa agagatgaac
    48301 cagcttcccg tattcataaa gcccaaggca ctgctggcct atgtcaatca aaatgaactg
    48361 cggcagcttg gaaatggatt cgatgcgttc gggaaggatc ttgggtcgta tggaaaagtt
    48421 cagcgggaca tagatgaact cgacatcggt gctcaggctg tcggtgcctc ctaccacggc
    48481 catcggttcg aggcgattga tcagggcgat ctgctgtcgg aaaaactggt agttgtggtc
    48541 gaagtactcc acgaaacgac aaaagtagtt catggattgg ccctttggga gaaatatctt
    48601 catgcagggg accccatcca cttcaacgat tttacggtcg gtgggaaact tgagaagttc
    48661 ggcatatagc cttccgtcaa tggctgccgg agcggcagcg gctgttgggg ataggcagca
    48721 cggagtcccc cagaggagca agaacaaaag ccatgttcgc gaataatgtc cccccacgat
    48781 tctccaatcc aatgcggtgc caaacggcca acaatgccat tctaccgcgt caggagccac
    48841 taacgcaaga gagggttgag aggttcgcat gatttctcta ctaaaacaag cacatggaca
    48901 gcatggacgg gttaggtgga cggaccaaat ggacggactg cgtggaaaaa gaggagggaa
    48961 aatgcccagt tgccgacaga gccgcagttt cacgatcccg ggcatcactt cgaccccccc
    49021 gcttgtatcc gatggcgtcg acctgggaat gaggcgagcc ccgcaggggc gggatgagga
    49081 accccggggc agtaccccgg ggtatggggt caacatccgt caacacgagc cccgaaaggg
    49141 cgggcccatt gttgtcagtt ccatatgaaa cgttcgtcgt agggtacccg gtattttcga
    49201 gaagcgaccc cgagtttcgg gtcggtagac acacgaggcc agcccggttg tcaagctact
    49261 gaaaactgaa ctttggcaga atgcatgtaa ct
//
LOCUS       DAOOVC010000013        48188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_466_length_48188_cov_7.005152, whole genome shotgun sequence.
ACCESSION   DAOOVC010000013 DAOOVC010000000
VERSION     DAOOVC010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 48188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48188
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_466_length_48188_cov_7.005152"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLM79_03240"
     CDS             <1..894
                     /locus_tag="PLM79_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HOV85348.1"
                     /translation="RLGLEAGRNRICRAVGISRRRSSLLMTGADMDNLVVRKARFKDL
                     AVYALVTAGVRGNALRMSCDEGLYYEPGTINIILMANRRLTSRARARAVISATEAKTA
                     ALQDMDIRSGGNPLRWQATGTGTDEMIVVEGTGARLDNAGGHCKLGELFARAVYEGVK
                     DAVFRQNGITTPRNVLRRLQERNLSPSDLVRQCPCLESMSESKGSLTVGMVEEILLEP
                     RYAAFMESALALSDARERGQIGELDLFEMWARSVAEEIAGGPLPEWKELVTKEDIPVI
                     VRVSFNAVLNGLSQRVKEVKH"
     gene            891..1745
                     /gene="tolQ"
                     /locus_tag="PLM79_03245"
     CDS             891..1745
                     /gene="tolQ"
                     /locus_tag="PLM79_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOV85349.1"
                     /translation="MIPTTLTAMAHVTAEPAGALSGSGGFGLVDMVLHAGPMVRTVMA
                     GLLCLSLISWGIIMAKSSRLRKVNRESENFADLFMKIKSIDLLYEECERFKDGHLAHI
                     FRVGCAELHRVSALLAGRGIEDPGKHADLLLENVDRAIQGGIISKRKRLQRLLPFLAT
                     TGSTAPFIGLFGTVWGIMTSFRDIGMKGSANLAVVAPGISEALVATAMGLAAAIPAVV
                     AYNHFSNRIQVMEEDMLQFTSDFVNMLKTDLLFRPGRGEEASEPVEIAGERRGICGRM
                     EVDPAQIR"
     gene            1758..2207
                     /locus_tag="PLM79_03250"
     CDS             1758..2207
                     /locus_tag="PLM79_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOV85350.1"
                     /translation="MAMRTDGARSRGRMLSEINVTPFVDVMLVLLIIFMVAAPMMIQG
                     IDVNLPVASSRPIPTETERLVVSITSDGKIRVNQDEIELETLGAELSALRKGCGEKQG
                     VVLRADRKVEYGLVAQVMGSIREAGIEQISMVMEPLGGWTKHPASHR"
     gene            2285..3205
                     /locus_tag="PLM79_03255"
     CDS             2285..3205
                     /locus_tag="PLM79_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOV85351.1"
                     /translation="MEARDYRRKGLFEDGIGKGMALSVLVHGVVFLFTLGILRLMPSR
                     KIDPTLCTVVLLSREGLGGGAMEGEEGAMRPGPREMKGVAGPGTAMRPEPEATHAVES
                     TEAVKETVSSVTLPQKVQKPLEKPKPRPRAVSGLPKKPQTVPHAAHAGPTAPRTNSEG
                     PGAPDGTPSTHGGRHGTGDAEGTGPGHSGEGTGSGTMAGTGGDRGPYTVSFGSQDGPR
                     FLNKVLPKYPRLARELGKEALVLLLVTIDEHGRLVDVRVSKRAGSGFDEEALRAVQCS
                     TFTPARRNGRPVACRAELPVHFVLKGPDRN"
     gene            3304..4179
                     /locus_tag="PLM79_03260"
     CDS             3304..4179
                     /locus_tag="PLM79_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HOV85352.1"
                     /translation="MIRLSVLVLGLMVAASCEAGPVEGSSGRKVLAPADPCRVVSLAP
                     SITEMVFALGKGDRLKGCTQHCDFPAEAGPLPRVGSYRHPDMERIAALKPDLCIATRD
                     GNPPDVLERLEALGIPVYVVNPRDLDAVVATITEIGRLLNAGPRGEELAGDLRTRIER
                     VKSRVACAKRRPKVFFQIGVVPIVSVGTNTFIHELITTAGGKNLAEGPTPYPRFSREQ
                     VLALQPEVIIITSMTRGQAFEQVRDEWNRWDSLPAVQNQRIFIVESNLLDRPTPRLIE
                     GLELLARLIHPELFQ"
     gene            4176..5192
                     /locus_tag="PLM79_03265"
     CDS             4176..5192
                     /locus_tag="PLM79_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV85353.1"
                     /translation="MNTTPLPISFLKRMLAVCLLLALLLMISAFLGLSIGSAGQNMKQ
                     VLAVLFGHGERDPTTANILWRIRLPRVFLAALVGSTLSLGGLVFQALLRNSLAEPYIL
                     GISGGSAVGAITGILLGLSRFPGVASLAFAGSMLTLFPVLAVGSGRAAMRKDALVLAG
                     VMVNAFCSSAIMFLLSLTQDSRLHNILFWLMGDLSLSDPNQVLVLALTLFPCFALLFL
                     LARPMNLLLMGEDTAANLGVNVRLISIVLLVVTSFMVSATVCHSGLLGFVGLVMPHLL
                     RLSFGPDHRLLVPACILGGGAYLVLCDLLSRSLPAQGEMPVGVITAMIGAPLFIFLLQ
                     RSKP"
     gene            5189..6034
                     /locus_tag="PLM79_03270"
     CDS             5189..6034
                     /locus_tag="PLM79_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85354.1"
                     /translation="MKPALVVQDLDHSYGQRHILDRVSFSVDQGEFFIVLGPNGSGKT
                     TLIKAASGAVRAQKGLVEILGHPLRTYSGRALARTIAVVPQSAPPDIPFTVAEVVLMG
                     RSPHLGLLGLERRRDRDIARQAMSFTHVEHLAERKLDELSAGERQRALLARAVCQQPR
                     IIVLDEPTASLDPAHQVHIMDLMEGLRREEGITVVMVSHDLNLAALYADRLLLLKEGR
                     VVSLGTPAEVLVFETLERAYGCVLLVDENPLKKVPRVTVVPENLLTGHTKSSRAMGKG
                     PGGSP"
     gene            complement(6169..7629)
                     /locus_tag="PLM79_03275"
     CDS             complement(6169..7629)
                     /locus_tag="PLM79_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOV85355.1"
                     /translation="MASTLSEKLADFIDSVRYEDLPGETVRSARLAFLDWLGSAARGG
                     LEAPAKMAAAVLRNQGGAPQSTLLPYPEKTSCLNAALANGIASHIVELDDVHRGSILH
                     AGAAILPAAFAAAEMVHADGRRLIEAVVAGYEVAVRLGEAVSPSHYFFWHNTGTCGTF
                     GSCAAAGKLLGLDRRQKVWALGNAGTQAAGLWEFLRDGAMSKHLHPGKAAYNGLLAAL
                     LAKEGFTGAEAVIEGEKGFCRAMAPAFDLSKITEGLGAPPYKIQENSFKIHASCRHTH
                     PAVDLVLDLSAKHGIDSREVLSVTVRTYRTALNIIRNPAPRTVFDARFSLPFCVALAL
                     KRGRCGLSDFTRENLLDSEIRTLMSRVHLEPDEELDALHPARWGAVVEIKTRSVGPFS
                     ARTDFPRGDPENPVDEETLVSKFRLLALPAWGQDKVNALSRTVLDLEDLEDTALLFDP
                     ASDRGPTRGFPAQSDRARRGSTETDARPEPKSSTPN"
     gene            complement(7642..8307)
                     /locus_tag="PLM79_03280"
     CDS             complement(7642..8307)
                     /locus_tag="PLM79_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOV85356.1"
                     /translation="MRVTLEICRFTPERDPGPRFEAFTVSVNGTRTVLDALLSAWQQD
                     PGLSFRRSCRSAICGSCAMLINGRPALACQTLVRNAVSGGNSVTLEPLPHFRPLKDLV
                     VDLEPLFESLKAVVPWLVLRGDYDGRMDPETAAKLEESAACILCGICRGDLGETAKGK
                     PEGVVKALRLAQDPRDALGEHRLRLLELSPEVLKLFVDRLPRHCPKGIKISEDIVQAA
                     RKL"
     gene            complement(8309..9994)
                     /locus_tag="PLM79_03285"
     CDS             complement(8309..9994)
                     /locus_tag="PLM79_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85357.1"
                     /translation="MITHEVLVVGGGLAGLMAALAVEDGVDVAVLSKIYPTRSHSGAA
                     QGGFNAVLSQEDSIDAHIFDTVKGSDYLGDQDAIEVLCSDGPSVIRELEKMGVPWTRR
                     ADGRIAQRSLGGAGFPRACFVADFSGHVVLHALYEHILKRGVKIYPEWHLLELLVEEG
                     QVAGALAYDLANARLEIFRSKQVVLATGGYGRAFSKTTNAHANTGDGMAVAYRAGAVL
                     SDMEFVQFHPTTLYGTNVLISEAARGEGGHLRNNTGERFMARYAPEKMELAPRDVVAR
                     AIFNEIREGRGFEDHSVQLDLTHLGEALVAKRLPQVRDLAIRYAGVDPATVPIPIEPA
                     QHYSMGGVRTDLWGLTTLPGLLAAGETANVSVHGANRLGGNSLLETVVFGRRAGKKAA
                     ESARNAPWPALSRETVSRSLEPWTSLLSTPPPASSKQDDLFAVRREMATLMTNQVGVF
                     RTGVELEDAVEKLRQLKERYESISPRGGIQPFNYALMDYLEVGHLLELSTLIARGALA
                     RTESRGAHFRVDYPRRDDENWLHHTFVRRKDAEPEFFRGPVAVTRHEPQKRGY"
     gene            10425..11783
                     /locus_tag="PLM79_03290"
     CDS             10425..11783
                     /locus_tag="PLM79_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HOV85358.1"
                     /translation="MAQTEFRVLSPTAILGYGFPRESFERGMEKRPDLIGVDAGSTDP
                     GPYYLGAGKSFTSRSAVKRDLEFILAAAVERSVPCIIGTAGGCGAREHVEWTESIIRE
                     IAGQKGYTFPMAVIHADVSKETVLEAMERQRVRPLPPAPEITVEDVRHCVRIVAQMGA
                     EPVLRALDTDCRVILCGRCYDPIPFAAPAVRAGFDPGLAVHMGKVLECAAIAATPGSG
                     CDCVMGTLYRDGFELESLNPQRRFTRVSTAAHTLYEKSDPYHLPGPGGTLDLEATRFE
                     ALDGGRVRVSGTRFVPADPYTVKLEGVKRTGYRSICIAGIRDPILVGRIDTVLEAVKE
                     EVRKDLDFTGPLLFHQYGKNGVMGAMEPEKDRGSHELGLVMEVIAPDQEQANAVCSYL
                     RSTLLHFGYPGRISTAGNLALLYSPSDIACGEVYEFSLYHLMEVDDPESLFPICVREV
                     KP"
     gene            11780..12112
                     /locus_tag="PLM79_03295"
     CDS             11780..12112
                     /locus_tag="PLM79_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HOV85359.1"
                     /translation="MKTVPLTELAAVIRSKNAGPYELTIDILFKNARHYRYLKEIHFF
                     TEELFARLYGVGVEQVLNLVYFDPASAVKCTLVRPMVSGTPGDADVYGAQQHAPVLGL
                     RVPLEEWP"
     gene            complement(12285..12701)
                     /locus_tag="PLM79_03300"
     CDS             complement(12285..12701)
                     /locus_tag="PLM79_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOV85360.1"
                     /translation="MVYQRTLTVSTSGHGDMHDLTDRVAQVVAASGIRTGIAHVFNVG
                     STGALGVIEFEPGLQRDLPELLDRLIPPSRNYGHEQAWHDGNGHSHLQATLLGPGLTV
                     PLSDGKLVLGTWQQIFHLECDIKGRQRKVVVTLVGE"
     gene            complement(12762..13775)
                     /locus_tag="PLM79_03305"
     CDS             complement(12762..13775)
                     /locus_tag="PLM79_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85361.1"
                     /translation="MSRIRLNGLKVLEGCTHIFDSHRDDKTTPELLCSRLAAARINVA
                     FLTHDAGRGARTAQVSLCMEPARGAAVSLDLTAARGERGLTRMPSEVALVTVFPYGQR
                     PDIPGALMRFLAREGIEPAGFAGSPSSLCILVPAGQSERFLRGLFDLFEFTAYPSLDD
                     WRAAFPDKNPAAKEIACSYEEQIISTYNAVRYRDLDLWRLEVPSSRLAGLGNALADMQ
                     VHGLSMPFFTGQPGPQEMLHFALCLDGNRRDQVGRVLSRDLSRVQAVCHEDVSAFVLH
                     GPHFGDRYGVVGALLTSLHRSGIQPLALSCAVSSMTVVVAGRDLDRALIALGSHFLVP
                     PSP"
     gene            complement(14509..15744)
                     /gene="fabF"
                     /locus_tag="PLM79_03310"
     CDS             complement(14509..15744)
                     /gene="fabF"
                     /locus_tag="PLM79_03310"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOV85362.1"
                     /translation="MIQVVVTGIGVFSSIGCDRETFWNSLVSGTSGIKGIRAFDASRH
                     KSRIASEVVPFEPEKFLNFKQVRRMARVSQLAGCAAFAAVRDAGLDLEREDPHRMGCV
                     VGSAAGDYYNLEQQYDRFKEKGPGFVNPLAIPKVIPNMPASNVGIALGIRGPNLGVST
                     ACATGAHAIGIALGILRQGLADVVLAGGAESAITPYVVDGYACMGVLSQRNDDPGTAS
                     RPFDADRDGFVIGEGAGVLVLETLSHARDRRADILAVLKGFGMSCDAYNIAIPEPEGT
                     AAAAAMANAVRDAGLNMEEIGHINAHGTSTKVNDKVEAKAIEKAFGERAKTVPVTSVK
                     SMIGHTLGAAGAIEAAATVLTLHRGVIPPTINYHTRDPECDLHIVANEAREAQVMAAI
                     SNSFGFGGQNGVVVFTRWS"
     gene            16100..16942
                     /locus_tag="PLM79_03315"
     CDS             16100..16942
                     /locus_tag="PLM79_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85363.1"
                     /translation="MECRITILCENTVQGMGFWGEHGFAALIETPGQAVLFDTGQGFG
                     LVPNALRLKKDLGGVSKVVLSHGHLDHVGGLLAFLGVKGPCPVVAHPDLFTERFWLMP
                     APGGGEEPVSIGMPWPRSYLTTRGAQFQWCRDFAQVAPNVFVTGEVPRKTLFELGSPK
                     FVVNHNGGFIPDPFLDDFSMVLKTSMGLVIVLGCAHAGMINVLQHAVEKTGEERIYAV
                     LGGTHLGLSPQPQLDPTIEALKKFDIGMLAVSHCTGQAPVARLASEFADRFAFGRVGF
                     VLEV"
     gene            17091..17600
                     /locus_tag="PLM79_03320"
     CDS             17091..17600
                     /locus_tag="PLM79_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HOV85364.1"
                     /translation="MKRKTKSLLVLAGLFPVFWGWTPEGRGFPSAGAARIAGCPDSPN
                     CVSSRDPPGRRWIAPLGLRGSPEEAFRCLKEILLQGPRTTLVVEEGGYLHAESRTALG
                     FVDDVEFEFDPESRSILLRSASRTGYWDLGVNRRRLEALRAAFESKCRQGGNSRSARD
                     RTSLPGNGR"
     gene            complement(17638..18027)
                     /locus_tag="PLM79_03325"
     CDS             complement(17638..18027)
                     /locus_tag="PLM79_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOV85365.1"
                     /translation="MDLKEYFENRKGFGVLSTADGTGRVNSAVYSRPHAMDDGSLAFL
                     MPDRLTHFNLQSNPRAAYLFREEGPGYKGKRLHLTKIREETDPDLVHTLRRRHSTPES
                     QEKEGDRFVVFFRVDKVLPLIGPGEEG"
     gene            complement(18173..19147)
                     /locus_tag="PLM79_03330"
     CDS             complement(18173..19147)
                     /locus_tag="PLM79_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOV85366.1"
                     /translation="MNDGSSEVFLTGASGFIGGRLLRALASRGVPTRCLVRSAEHFPT
                     RGFCGTQPRVIRGDLLQPETFRGAMEGTHTAYYLVHSMGGRSAFRYKDYIRKDLDAAR
                     NFARAAEESGVRRIVYLGGLGEVADNLSEHLRSRQEVARLLQSSRVPVTVLRAANIIG
                     AGGAPFEMLRHLVERLPVMICPRWIDTRCQPIAVENVIEYLEGCLDEPKTAGRTFDIG
                     GAEILTYRQLMQTYARVRHLRRWIFTVPVLTPHLSSYWITLVSSVPAGVVMPLVEGLK
                     NEVVCRDHAIRELIPIRLLSMEEAVRNALGELARGPGGLPSCQSCLRN"
     gene            19346..19969
                     /locus_tag="PLM79_03335"
     CDS             19346..19969
                     /locus_tag="PLM79_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV85367.1"
                     /translation="MEERKLSRRERERLAQRREMLAAALDLFSDKGYHNVSMHQIAEK
                     AEFAIGTLYRFFKNKEDLYRALIREQSDKFHEALTRAIEEPGDEVEKLRRYVRAKSEV
                     FRANVPTIRLYFAETHGARFNIMAGVDSEIRERYRQFQQTLAGVFESGMERERFQRIA
                     TPYSLAVALNSITNAFLSLWLEDPEHHPYPEDPDTILNILFKGLMNE"
     gene            20037..21251
                     /locus_tag="PLM79_03340"
     CDS             20037..21251
                     /locus_tag="PLM79_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV85368.1"
                     /translation="MQRNETVNGPRTILVVWMVLLVGLLAGGCDRRQQAPPPPAVPEV
                     VVETVRTHRIVLTTELPGRTAPYLVAEIRPQVNGLIQKRLFKEGSDVKVGQVLYQIEP
                     APFQAALDNAKAGLARAEANLPAARLRAERYKELLADKAVSRQDYDDAAAALKQAEAD
                     VEYWKASVETARINLGYTRVTAPISGRIGRSNVTDGALVTAHQPLALATIQQLDPIYA
                     DVPQSTTDLLRLRRRLEDGRLNENGTDQRKVRLILEDGSSYPREGTLQFREVTVDPST
                     GSVILRIVFPNPEGVLLPGMFVRAVVQEGINQQAILIPQQTVVRDPKGNPSVLIVNSE
                     DKVEQRMITLDRDIGDQWLVSSGLAPGDRIILEGMQKVRPGVPVKVVATGAGEPEPGK
                     PGQPSQPAAKSN"
     gene            21264..24446
                     /locus_tag="PLM79_03345"
     CDS             21264..24446
                     /locus_tag="PLM79_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV85369.1"
                     /translation="MLSRFFLDRPVFAWVIAIIMMVAGGLAIYNLPISQYPPIAPPSI
                     AIDSFYAGASAETVENSVTQIIEQKMTGFDKMLYLKATSDSSGSSRIELTFVPGTDPD
                     LAWAQVQNKLQLAMANLPEVVQKSGVKVSKSTRNWLILVGLISEDGSMDGNDLRDYAK
                     SNLEKVLSRVPGVGEVEAFGTEYAMRIWVDPDKLINYQMTVEDVMAALEAYNVEISAG
                     QFGGAPAVDGQRLNASIIVQSLLRTPDEFASIPIRLNPDGSTVRIRDVARVELGTETY
                     DVVAYHNGKPAAALAIRQAAGANAIDTADAIKAKMEEMSRYFPPGMKVVYPYDTTPFV
                     KVAIKEVVKTLFEAIVLVFLVMYVFMGSIRATFIPTIAVPVVILGTFGVLGWLGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERIMAEEGLSPREATRKSMDEITSALIGIGLVL
                     SAVFGPMAFFGGSTGVIYRQFSVTIISSMLLSVVVALILTPVLCASLLKPVPKGHQPS
                     DGAVFFLRPFFRWFDRFFLGSRRLYLRVVGHALAKKFRYLLVFLLIVAALGYLFRKMP
                     TAYLPDEDQGTMYVQAMLPANSTLEQTQQVMDGVREYFLEKEKDAVETVLTVAGIAFA
                     GRGQNMAIAFVRLKDWELRQRPDLKVGAVAGRAMKAFSQIRNAIVVAFPPPAVVELGT
                     AKGFDFQLLDRGGLGHEALMQARNQLLGMAAADPRLQAVRPNGMEDVPQYRIDVDWEK
                     AGALGVPITSIHRTISTAFGSSYINDFIQSGRVKRVYVQADAPFRMLPKHLGELYVRN
                     SSGKMVPFSSFASGHWFSGSPTLERYNGFPSINIWGEAAAGRSSGTAMKAMEEIASQL
                     PQGFGYDWTGLSFQERLAGAQAPVLYAFSILVIFLCLAALYESWPIPISILMALPLGA
                     IGGVIGSTLNRLPNDVYFQIGLLTTLGLTTKNAILIVQFAEAKVEEGMDLIHATLEGA
                     RLRLRPIVMTSLAFGFGVLPLALAGGAGAGAQRAIGIGVLGGVVSATFLVTLFAPLFY
                     VIITKMSRKHRRRVAAKTVEATPSGDQGHE"
     gene            24439..25893
                     /locus_tag="PLM79_03350"
     CDS             24439..25893
                     /locus_tag="PLM79_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOV85370.1"
                     /translation="MNRNRLLLLGVIVTFLGGCTPAPKYTRPGAPIPGDWPDGAAYQD
                     SRAVPDAPAPTALSWREFFGDPRLQKIMETALRNNRDLRLAVLNVERARAMYGVQRAE
                     LFPVINAVGAGSKYRLPADLSSSGKSTTSERYDVNVGIAAWEIDLFGRIRSLKDRALE
                     EYFATEQARRAAQILLLSEIARAYLALAADRESLQLAQFTLESQQEGYDLIRRRYEKG
                     MATELDLRQEQTRVEAARVDVALFTRLIAQGENALDLLAGSPVPKDLFPANLEDVGPH
                     PEVAAGISSEVLLNRPDILEAEHRLKAVNAAIGAARAALFPRISLTTAVGTASSDLTG
                     LFQAGSAAWTYAPQIVLPIFDARAWAALEAVKAERKIVLTQYEKAIQTAFREVADTLA
                     VKGTIDDQLTSQEALVFASAETYRLSNARYMKGIDSFLGVLVAQRSLYAAQLRLIAIR
                     YERLANQVRLYAVLGGGADPAVPRAEAPGRNAVD"
     gene            25989..27998
                     /locus_tag="PLM79_03355"
     CDS             25989..27998
                     /locus_tag="PLM79_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOV85371.1"
                     /translation="MKGQPAEVRFMGIASDLVIIVVAALVGGIVAHKLKQPPILGYIM
                     AGMAVGPYSAGATLSDIHDIELLAEIGVALLLFALGLEFSLKELKPVRRIALVGTPIQ
                     MVLTMGYGFLVGQWLGWEPVPSLWLGALISLSSTMVLLKTLMNQGWLGTLSSRVMIGM
                     LIVQDLAVVPLMILLPQLSNPKAGIPLLGLAALKAAAFLFFMVLLGSRLLPRLMAYIA
                     GWNSRELFLLAITAIGLGVGYATYLSGLSFAFGAFVAGMVLSESDYSHQALADIIPLR
                     DIFGLLFFTSVGMLLDPSFLVSHWNTILLLVVLTSVGKGFLFAALGHAFGYRNVIPLA
                     LGLGLFQIGEFSFVLARLGVSTGSISNEAYSLVLSTAVTTMVLTPFVSGLTAPLYSLR
                     KRRFKHEPVQTINLPQSGLYDHIVIAGGGRVGLHVARVLRTLGHPFALIDLNYRQVEE
                     AKRDGFPVIYGDASQEVVLEAAHLDKARLLLITIPAVVVSQAIVDLARQIKPTLSIVA
                     RTEGIEQMKALHAKGVAELVLPEFEAGLELTRQALRHLHVPASQILRYTDTVRRDLYG
                     PLYENDHDHDKLSFPRSAGTLLELNWFALEPDSFLIGRTIGELEIRTRTGVSVVGVMR
                     DRDLHPNPGPDFRFGSGDFLAVIGKPEQFDAFERFAGAGSGPDPG"
     gene            28040..28531
                     /locus_tag="PLM79_03360"
     CDS             28040..28531
                     /locus_tag="PLM79_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOV85372.1"
                     /translation="MRVSRIPRRLTTSAAFILLMTGLVSPGLASVKLVKGQTVYVPAY
                     SHIYYGDRELPFYLTVTLSIRNTDPTHPITLVSVDYFDSDGKRLQSHLAKELKLNPMS
                     STRFVVKESDKSGGSGASFLVTWKSDKKVCEPVIETVMISTSTQQGISFSSRGKPVKE
                     LED"
     gene            28647..29813
                     /locus_tag="PLM79_03365"
     CDS             28647..29813
                     /locus_tag="PLM79_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85373.1"
                     /translation="MNRNLKILVAGYAIGFPLGGQLWMMLQYVLGLKRLGHTVLFLED
                     TSDWSYPYDPVRRSYDLGSSYGRRVLDDFFARCGLKGQWVYNAPIENAVYGMSREDLD
                     RFCSQADLFLNISGITPLREEYLRCRVKAIIDTDPVFNQIKISNDEKSREYFQSHDFC
                     FTFGWNLPDGVAGVPHSGIDWKSTLPPILPDLWVPLETPGGAFTTVGSWDSRGRDVLL
                     NGRVLSWRKRPKYHQLLDLPRHLPGVDLELAFLGIEDDAKLFAEHGWVIRDAVEVSAD
                     PWRYRDYLRNSRGEFTVAKDQNVITRSGWFSDRSASYLAAGRPVITEDTGFGSYLPVG
                     EGLFTYKTLEEAVQCIRAVEADPVRHFRAARRIAQEHFDAPKILSRILADAGLA"
     gene            30075..31310
                     /locus_tag="PLM79_03370"
     CDS             30075..31310
                     /locus_tag="PLM79_03370"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOV85374.1"
                     /translation="MVSYPKALIDCYSRLIARAEKTFVPGKPRARSTKGDAVESLPSQ
                     RLLSLDVFRGAAIVGMILVNNPGRWRVVYSQLQHAPWNGWTFTDMIFPFFLFSVGISI
                     TFSFTRRIEQGHARSTLVFQAMKRTVLLFLLGIFLSNAPHFHMDNLRIPGVLQRIALC
                     YFISSVLFLGSGWKGQASWMAALLLAYWLMTALIPVPGIGAGVYEPGRNFAAYVDSIF
                     LENHMWSAYRSWDPEGIVSTLPAVSSTLFGVLAGHCLRTAHSIPRKTLGMLAAGVLFL
                     LLGLALDPLLPINKSLWTSTYCVFMTGWALLCFGVFYWLIDGKGYRKWAFPFVVFGTN
                     AIFAYGLSKLLDDLLKLVTIAPQGGGPRIRLKHCIFDFLNALAGTSKLASLLYAVLNV
                     LAVFLVVYGLYRKRWFLKL"
     gene            complement(31895..32965)
                     /locus_tag="PLM79_03375"
     CDS             complement(31895..32965)
                     /locus_tag="PLM79_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOV85375.1"
                     /translation="MKPASRKAWRFICVVMPVLSLLLFPFLARADLPVVTLIATGGTI
                     AMKIDPVKKAPVPAISGEDLLSTVPEIGEFARIEVQNLSNVPSDYMDPERWIGLQKAV
                     VAAMARPEVVGVIVSHGTDTLEETAYFLDLTVPGEKPIVLIGAQRNASEKDFDGPRNL
                     LNAARVCVSPDAKGKGSMIVLNNQINAAREVVKTHTSDVETFKSGDFGFLGTADNDRV
                     IFYRSPLRRQHIPLTKATLPYVEIVTMYGGADGKLVKAAVDAGAKGIVIQALGWGNVN
                     VPMFEAIKEAMGKGVPVVISTRVPNGRVLPVYGFQGGGKTLKEAGAVFADNLPPQKAR
                     ILLMLALQETPKADRLQELFDR"
     gene            complement(33304..35037)
                     /locus_tag="PLM79_03380"
     CDS             complement(33304..35037)
                     /locus_tag="PLM79_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOV85376.1"
                     /translation="MEETSKNGGILYAVDESPPLWMVILLAFQHVVIMYGENTLFPGI
                     LGRMANAPEEHIAFATFGAVVISAVSSLLQLIRFGKFGSGFVIFMGSSTAYLACSLDA
                     LHAGGFALLATLSVLSAPAELLVSYFLRHLRTIITPPVGGIVILLIVISLMPVCLKEW
                     MGHPGEPHYASRESLAVGMITLFTMLSINLYAGKKLKVWGPVLALGVGYVAAWTFGIL
                     DFEHFKGSEWMGLPRGNWPGLTLDLRWEHLPLLAAFVVVTVVNSIQAIGNCMLAQSYS
                     VRDFKKVDYDRVQGCLYADGTANILAGLAGTLPNETYGENVPVLGMTGVASRSVGFLA
                     VGFLFFLAFCPKISALILDMPGPVFGGFLVGLLALMFHSGLHLIHQSGMSPQMGLLIG
                     VSMCVGMMAESREFFSGTMPAALQPFTTRAVAAGGLMALVLNTIYHFQPKPRVSFKVE
                     GVPEKLPDLVRQLADGVESLQLTPSQASILQLSCEEVFQHIQSESESEGVPKRRVKFR
                     IVRLEEGVFVEIRAATRMSDVDGPARPETFAWASEEDCKALGLYLIRNMVRQIRHVHI
                     SGYTYVSFIIP"
     gene            35320..35925
                     /locus_tag="PLM79_03385"
     CDS             35320..35925
                     /locus_tag="PLM79_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85377.1"
                     /translation="MKKARSKRFLVFVTFLLAVTFAAMAALCSADDHKRKRDHSRKHD
                     PTSWSEGFGRFSGKHHDEGNETTGQVAAWSFAAANLGVSLSLLIKGVNRFSPLSPQWK
                     NALKKFNGTRKKYSMKPHYLLNPIILALALLHYSLSRCPSTSLPEWGLLGIALMMTLG
                     IVMKLRLCPNSLLKSVYKVHTQPVAVLFVISLLVIGHMIAD"
     gene            complement(36100..37077)
                     /locus_tag="PLM79_03390"
     CDS             complement(36100..37077)
                     /locus_tag="PLM79_03390"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOV85378.1"
                     /translation="MQQLGMGQAVHQALREEMIRDERVFVAGEGVGVSIHDSPMLPTA
                     GLLKEFGPGRVKDTPVSEAAIAGLAVGAAVAGLRPVVEIMFNPFFTLASDMIVNHAAK
                     LRYLSGGKSTFPLVVRMKSGAGFGAGCQHAHNLEAWVAHSPGLKVVMPSTPADAKGLL
                     KSAVRDENPVIFIEDMLLYFVPGPVPEEEYLTPIGKADVKRPGRDVTVVTWSKMLGVA
                     LKASAQLAREGIEVEVVDVRTLVPLDKETLLASVRKTGRLVVLHEATRTGGFAGEICA
                     LVAEEAFSSLKAPLRRVTAPDIPVPVSPPLERFFIPDEAKLIAAIREVL"
     gene            complement(37122..38087)
                     /locus_tag="PLM79_03395"
     CDS             complement(37122..38087)
                     /locus_tag="PLM79_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOV85379.1"
                     /translation="MEITKEKKLEMLRSMLLTRRFEETLTELCKIEGKIPGMMILSTG
                     QEAVGSGVCAALLPEDILISNHRSHSHLLAKGADPKALMAEIYGKRTGCNKGKSGTLH
                     LAVPEVNALCTTTVVGGGLPIAVGTAFSQQYRKEKTVTACIFGDGAADEGSFHEALNL
                     ASLWSLPVIFVCENNLYAGAQRYEEHTRVNNIAERASAYAMPGETADGNDAPAVYEAT
                     RRARERAVSGEGPTLIECKTYRCRGHGESDHQHYQPKDEIAAWQAKCPIPRLRDEVLD
                     RGWMTRQELEDLESRVREIVAEAVRFAEESPYPDPLDALEDVLVS"
     gene            complement(38335..40050)
                     /locus_tag="PLM79_03400"
     CDS             complement(38335..40050)
                     /locus_tag="PLM79_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOV85380.1"
                     /translation="MGLLKSWRGVKEIKRLGTITRVLLKHGFRDVAERLWGAKAGRPE
                     KDPEEGGLLVRGFPSPRRIRLVLEELGPSFVKLGQLMSTRADIFPPEYIAEFEKLQDR
                     VPPIPFKDVKAVIEKELRRPLDQIYARFDPDSIAAASVGQVHTALLFSGEKVAVKIIR
                     PGIESKIRDDIRLMYFIAHRIEKSFEIGHMIGVVNLVQEFERVIFRELDMLIEAGSME
                     KFAYNFREVDEIHIPRVYWDYTTRSVLVMEHVDGIKMDQVDAIRAHGIDPKEIALIGL
                     RTFSRQLMEFGFFHADPHPGNTIVLYDGRVSLVDFGITGYLDEETMRQIAHLFLGFAQ
                     HDYDLVMEALLDAGILNEDLMDLRAFRIDLKDVSEAFYGRSLQNISVRDVYDQLMHLV
                     LKYNIRLPRNILLLLKTFIQTEALGKILGSDASILEVTRPYAKKLLKQGVEARSMRKD
                     LAKDARTAGLYMKAFPKHVHDILKAASRGKQRVELRHDGFDRLDAQLEKGVNRLTVGM
                     IIAASILAAALVLNSDQKVMEFALNFFGPQTLSLTSLFGLAGYIIATVLGLWLILSIL
                     RSGKL"
     gene            complement(40243..40977)
                     /locus_tag="PLM79_03405"
     CDS             complement(40243..40977)
                     /locus_tag="PLM79_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV85381.1"
                     /translation="MKGCLAVYHREVLILRKKLVKQVASMAVAPLLYVLAFGLGMGRH
                     VEIQGRSYLEFLIPGLVAMSSMTQAFAIASEINIARFYWRIFEEFQAAPIGSMAYVTG
                     EVLAGITRALLSVLVILAIALSFGVVLSYSPAFWLGVVLNSFVFASLAVGMAMLVKSH
                     ADQALLTNFIITPMAFLGGTFFPVDQMPSWVQKPLGLLPLTHAAHAVRTAAFEGAPEA
                     FPYVLLASMGVVLFVFALFCVNKAKE"
     gene            complement(40974..41894)
                     /locus_tag="PLM79_03410"
     CDS             complement(40974..41894)
                     /locus_tag="PLM79_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85382.1"
                     /translation="MKRKCEDLHPAQWGRNPRNGTGWIAPEMIRIENLTKNYKSVQAL
                     KGISLHVAEGELFAYLGPNGSGKTTTIRVLTGLAAPTSGNATLHGFHILRDTLKAKRQ
                     CGLVPQAINLDQELTVRENLEIHGRLFQMPRSERKSRIEELLDYIGMKDRRDSPVKHL
                     SGGMRRRVMIARALMHSPRILFLDEPTVGLDPTIRRKIWSLIKRIQQSGATIFLTTHY
                     IEEAEFLAERVAFLDEGRIVAVDSPQALMARLGTWALDEMVDDNMETRYFQSRGEANA
                     YIARQQGSFSLRRVNLEDVFLSLTGKKVLP"
     gene            42352..45435
                     /locus_tag="PLM79_03415"
     CDS             42352..45435
                     /locus_tag="PLM79_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOV85383.1"
                     /translation="MAYDLGVDVGSVSINSVLVDEDQRVVYEAPYRRHFGLVFEETLA
                     FLRRILDPSFFAAEGIPSQPIRSVSFTGVHGQLLADVLSAPYEVETIAQALGVMHLVP
                     GVRSIISMGGQDAALLQLTYVGDRWHLEAFNMNGPCASGTGSFIDQQAERLAQSLYGP
                     EFHMDQEKLQKTLDDFIELGLKSTYPAPVATRCTVFTKSDMIHLQNKGEPLPNIIAGL
                     HYGNAANYLSTIVANRELEDPVVFVGGMASNRLQVMAFQHYFPGLRVPAHHTSLGALG
                     LALQTQRQGTRVQIDLSRLKEYQSALQEEFPVAPRLELEWTRFDPDNSLSARHKRRKK
                     NPRVYLGVDIGSTTTKYALIDGKGEILHKCYVPTQGKPIEVTQRLLQNLVRETGKQVQ
                     LAAIATTGSGRNVVGDFLKADLIIDEITAHARGAVEVDPLIDTIFEIGGQDSKYIRIE
                     NTHPLDFDMNKVCAAGTGSFLHELANKMKINIVGEFQEIALSARTPIHLAERCTVFME
                     SDLGSYAQKGARREDLIAGLCYAIVHNYLNRVVEKRPIGQRVMFLGGPSLNRGIVAAF
                     ERVLQRPILVPKHREVLGAFGAALAVREMFEKQELSRQSRDLEALARMEVSYTESICR
                     ADKKCHNECKLKVYDFGGQKSVWGGDCGRYEVTRSTGWVRTNLFLEYQQFFQEMMDEE
                     GISAHEVSPGDRATKPRIGIPMALHAVEWGVFWAVLFSQLGHSVVFSPRTNNRLALSG
                     VECMTAETCFPVKVFHGHVRHLMEQADYLFLPNVINAPTPQEDEIGFFCPLVESSQYM
                     SRAALKVPDPRIIRPTLHLKDGPEHLSHAFHKGFPPELRPSLRELETAVQRAWDAQHA
                     FRARLLRRGREVLEQLPPGEPLWIVTGRPYNLYDERLNLQLGRNLAKRNITAVPMDFL
                     DLNAEDLSDFPQMYWGLGARVLRAAKVIARTPHWYGVHLTNFSCGADSFIEHFYRHIL
                     GEKPALVLELDEHSAVAGLLTRIEAYQNVVKNLEEQSSICDPNPQSRQAGLEATG"
     gene            45465..46835
                     /locus_tag="PLM79_03420"
     CDS             45465..46835
                     /locus_tag="PLM79_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA activase"
                     /protein_id="HOV85384.1"
                     /translation="MANPQTVASFERLKTSVAQFDVSGRTLLIPNMAPTGVRLLAACF
                     RAVGVNAVVMETYKGLNLGKEFTSGKECFPCQVTLGDILYHLKKEEERLGEDFSPGNY
                     VYFLPEADGPCRFGMYNKMQRLVLDRFPTYKDLPIVYLSTGNSYATSDILSPRYAPIF
                     RRLSYVALIIADVFDRTVWRVRPYERRPGMTDEFMDGAIEEMAALIERIGTTLDFPQL
                     YRCLETTVATAAGFIDPQKPRRPRIGIVGEIYLRTHPDSNQEIIRQIEKFGGEVVDAS
                     LGEWVNYVTYEQLQKTARKWRLAWRERDHGVLRKLTLQWISLQIERWYQAWRQGQVYR
                     IAMRHLDIQQDHDIAAVEKRLDRDRLYSFAIGTEAALSIGGALEYVHEGFDGVVNVFP
                     FTCMPSTICASILKPLLLDKRIPYLDAPYDGTYQPNREIVLRTFLYQARQHMESRRAK
                     RENGKN"
     gene            46837..>48188
                     /locus_tag="PLM79_03425"
     CDS             46837..>48188
                     /locus_tag="PLM79_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOV85385.1"
                     /translation="MSNQTMIINAAFPEECRVAILEGQSLEGLFLDTASRTKIVGNLY
                     KGVVTHIQTSLQAAFVNYGMERNGFLPLSEIHPEYYQQEPSEPLRIQDVLRVGQEVLI
                     QVVKEEVGTKGALLTTYVSLAGRDVVLMPGQPQRGVSRKIEDETHRERLKQLAHDLRI
                     PEEFGIIIRTAAEDRTKREVAKDLNNLMKMWEEIKKRVQETPSPGLIHQEQDLALRVI
                     RDYFNPNIKTVLVDDRETHQRVKDFLRIIDPRLQRIVKYHREDAPISLKYNLEDQIEQ
                     VFQKRVPLKSGGYLLIDSTEALVAVDVNSGRANGDGAVEEMVFKVNMEAATEIPRQLR
                     LRDLGGLVVIDFIDMHDRRHTREVERRLKEEMKKDKAKITIGRISRFGLLELSRQHLG
                     INTLRSSSRECPTCKGAGWVRTPLASALFHLRKIWIGLAQRKDLAQVRGTFSIEVANY
                     L"
BASE COUNT         9272 a        15007 c        14624 g         9285 t
ORIGIN      
        1 aggcttggcc tcgaggccgg gaggaaccgc atctgcaggg ccgtcgggat atcccgccgg
       61 cgcagcagcc tcctcatgac gggtgccgac atggacaacc tcgtggtccg caaggcccgg
      121 ttcaaagacc tggccgtcta tgccctggta acggccggag tccggggcaa cgcccttcgc
      181 atgtcctgcg atgaaggtct ttattatgag ccgggcacca tcaacatcat cctcatggcc
      241 aacaggaggc tcacatcacg ggccagggcc agggccgtca tcagcgccac ggaggccaaa
      301 accgcagccc tgcaggacat ggacatccgc agcggcggga acccgctcag gtggcaggca
      361 accggtacgg gcaccgacga aatgattgtg gtggaaggga cgggtgcacg cctggataac
      421 gcgggaggcc attgcaagct gggggagctc ttcgcccgag cggtctacga aggggtcaag
      481 gatgcggtct ttcgtcagaa cgggatcact acgccgcgca atgtgcttcg acgattgcag
      541 gaaaggaacc tcagcccctc cgacctcgtg cgccaatgcc cgtgtctcga gagcatgagt
      601 gagtcaaagg ggagccttac cgtgggaatg gtcgaggaga ttttgcttga gccgcgttac
      661 gccgctttca tggaatcggc cctcgcgctg agcgatgccc gggaaagggg gcaaatcggg
      721 gagctggacc tcttcgagat gtgggcgcga tccgttgcgg aagaaattgc cggagggccc
      781 cttccggaat ggaaagagct cgtaacgaag gaggacatcc ccgtgatcgt cagggtgtct
      841 ttcaacgccg tgctgaacgg cctttcccaa agggtgaagg aggtgaagca ttgattccga
      901 ctacgttgac ggcaatggcc cacgtgaccg cggaaccggc aggtgccctt tccggttcgg
      961 gcggattcgg cctggtcgac atggtgctcc acgcggggcc catggtccgg accgtcatgg
     1021 cgggcctgct ctgtttgtct ctgatcagct ggggcatcat catggccaag agcagccggc
     1081 ttcgaaaggt aaaccgcgaa tcggagaact ttgcggacct tttcatgaag atcaagagca
     1141 tcgacctgct ctacgaggag tgtgagcggt tcaaagacgg ccacctcgcc catatcttcc
     1201 gggtcggctg cgcggaactg caccgcgtga gcgcattgct tgcgggcaga ggcatcgagg
     1261 accccggaaa gcatgcagac ctgcttctcg agaatgtgga tcgggccatt cagggcggca
     1321 tcatctcgaa acggaagcgg ttgcagcgcc tgctgccttt tctggctacc acgggcagca
     1381 cggccccctt catcggtctt ttcggcaccg tgtggggcat catgaccagc ttccgggaca
     1441 tcggcatgaa gggctcggcc aacctggcgg tggtggcccc gggcatctcc gaagccctgg
     1501 tggccacggc catggggctt gcggctgcca ttccggcagt ggtggcctat aaccacttct
     1561 cgaacaggat ccaggtgatg gaagaggaca tgctccagtt cacctccgat ttcgtcaaca
     1621 tgctcaagac cgacttgctg tttcgccccg gccgggggga ggaggcgtcc gaacccgtgg
     1681 agatcgccgg ggagcgacga ggcatctgcg gccgcatgga agtggacccc gcgcagatac
     1741 gatgaggagt gcttccgatg gctatgcgga cggacggggc ccgctcccgc gggcgaatgc
     1801 tctctgaaat caatgtgact ccctttgtgg acgtcatgct cgttttgctc atcatcttca
     1861 tggtcgcggc acccatgatg atccagggca tcgatgtgaa tcttcccgtt gcttcttccc
     1921 gtcccattcc caccgagacg gaaaggctcg tggtgagcat cacctcagac gggaagatcc
     1981 gcgtcaacca agacgaaatc gagcttgaaa cgctgggcgc cgagctctcc gccctccgga
     2041 aaggctgcgg cgaaaagcag ggagtcgtct tgcgagcgga ccggaaggtc gagtacggac
     2101 tggtggccca ggtcatggga agcatccggg aagcgggaat cgaacagatc tccatggtga
     2161 tggaacccct ggggggatgg accaagcacc cagcgtcaca caggtgagcc atcaggccgc
     2221 tcaatcgagc gggtcatgaa agcgtgaacg gagcacacac ggaaaggcat cgaagggcaa
     2281 cggaatggag gctcgggatt acaggaggaa aggcctgttc gaggacggca ttgggaaagg
     2341 gatggctctg tccgttcttg tccacggggt ggtcttcctg ttcacattgg gcattttgcg
     2401 gctgatgcct tctcgaaaga tcgatccaac gctttgcacg gtggttctcc tcagccggga
     2461 gggcctcgga ggtggggcga tggaaggcga agagggcgcc atgagaccag gaccccggga
     2521 gatgaagggt gtggcgggcc cgggaaccgc aatgagaccc gaaccggaag cgacccacgc
     2581 ggtggaatcc accgaagccg tgaaggaaac cgtgtcttcc gttacgctcc ctcaaaaggt
     2641 ccagaagcct ttggagaagc ccaaaccaag gccgagggcc gtgtccgggc tcccaaagaa
     2701 accgcagacc gttccccatg cggcccatgc cgggcccact gctccgagaa cgaacagtga
     2761 gggtccgggc gcccccgacg gcactccttc cacccatggg ggacgtcacg gaacgggaga
     2821 tgccgagggc acaggaccgg gccactccgg agaaggaaca ggctcgggca ccatggccgg
     2881 caccggtggc gaccggggac cgtacacggt atccttcggt tcccaagacg gtccccggtt
     2941 cctcaacaag gtcctgccga agtacccccg gctcgccaga gaactcggaa aggaagcgct
     3001 tgtgctgctc ctggtcacca tagacgagca cggacgtctc gtcgatgttc gagtgtccaa
     3061 acgtgccggg tcgggattcg acgaggaagc cctgcgggcc gtccaatgct cgaccttcac
     3121 cccggcgagg aggaacggaa gaccggtggc ctgcagggct gagctgcccg ttcacttcgt
     3181 actgaagggg cccgaccgca attgatttca ccctcggggg cggcacacac ccgtcggctt
     3241 ggccgtcctg tgccgttccc tcaggagctc gctccctgct ttggaaagga accctttgac
     3301 cccatgatcc gattgtctgt tttagtgctt ggcctgatgg ttgccgcgag ttgtgaagcg
     3361 ggaccggtgg agggttcatc ggggcggaaa gttttggctc ccgccgatcc ctgtcgcgtg
     3421 gtgagcctgg cgcccagcat tacggagatg gtctttgccc tgggaaaagg cgatcgcctg
     3481 aaaggatgta ctcagcactg cgattttccc gcagaggcgg gacccttgcc aagggtgggc
     3541 tcttacaggc atccggatat ggaaaggatc gcggcattga agccggacct gtgcattgcc
     3601 acccgggacg gcaacccgcc cgacgttctg gaaagacttg aggccctggg aatcccggtc
     3661 tatgtggtga acccgagaga cctcgatgcg gtcgtagcca ccattacgga aatcggccgt
     3721 ctcttgaatg ccggtccccg aggagaggaa ctggcaggcg atctgcgcac caggatcgaa
     3781 cgggtgaaat cgcgtgtggc ctgcgccaaa cggcgcccga aggtcttctt tcagatcggg
     3841 gtcgttccca tcgtttccgt gggaaccaac acctttatcc acgaactcat caccacggcg
     3901 ggaggaaaaa acctggccga aggccccaca ccgtatcccc gcttcagccg ggagcaggtc
     3961 ctggcgctcc aacccgaggt gatcatcatc acctccatga cgagaggcca ggctttcgaa
     4021 caggtcagag acgagtggaa ccggtgggac agtctgccgg cggtccaaaa tcagcgcatc
     4081 ttcatcgtgg aatcgaacct gttggaccgt cccaccccga ggctcatcga aggcctcgag
     4141 ctcctggccc ggctcattca cccggaactg ttccaatgaa tacgacgccc ctgccgatct
     4201 cctttctcaa acggatgttg gctgtttgcc tgttgcttgc gctgcttctc atgatcagcg
     4261 cctttctcgg gctttccatc ggatctgcgg gacagaatat gaagcaggtg cttgccgtgc
     4321 tcttcggcca tggagaacgg gaccccacca cggcgaacat cctttggcgc attcgcctgc
     4381 cgcgggtctt cctggccgcc ctggtggggt ccaccctctc cttggggggg cttgtcttcc
     4441 aggccctttt gcgcaactca ctggccgaac cttacatcct gggcatctcg ggcggatctg
     4501 ccgtgggggc cattacgggc atcctcctgg gattgtcgcg gtttccgggt gtcgcctccc
     4561 tcgccttcgc aggcagcatg ctgaccctct tcccggtgct cgccgtcggc tcgggcaggg
     4621 cggccatgag gaaggatgca ctggtactgg ccggagtcat ggtcaatgcc ttctgctctt
     4681 cagccatcat gtttctcctc tcactgaccc aggactcccg gctccacaac atcctcttct
     4741 ggttgatggg cgatctctcc ctgtccgacc caaaccaggt cctcgtgctt gctctgacgc
     4801 tctttccttg cttcgctctg ctcttcctgc ttgcccgccc catgaatctt ctcctcatgg
     4861 gcgaggacac ggccgcgaac ttgggagtga acgtgcgcct catatccatt gtgctcctgg
     4921 tcgtcacttc tttcatggtg agcgctacgg tatgccattc ggggcttctg ggctttgtgg
     4981 ggttggtcat gccccatctg ctgaggcttt ctttcgggcc ggaccaccgg ctgctggtcc
     5041 ctgcttgtat tctgggcggc ggggcctacc tggttctctg cgacctgctc tcgagatcgc
     5101 ttcccgccca gggagaaatg ccggtggggg tgatcacggc catgatcgga gcgcctcttt
     5161 tcatctttct gctgcagagg tccaagccat gaaaccggcc ctggttgtgc aagacctgga
     5221 tcattcttac ggacaaaggc acatcctgga ccgggtctcc ttttcggtcg atcaggggga
     5281 gtttttcatc gtcctcgggc cgaacggatc gggcaaaacg acgctgatca aggccgcctc
     5341 gggagccgtc cgggcgcaaa aagggcttgt ggaaatcctg ggacatccgc tccgaaccta
     5401 ctcgggaagg gccctggccc gaaccattgc ggtggtgccc cagtcggcgc ccccggacat
     5461 tcctttcacg gtggccgaag tggtgctcat ggggcgttcg cctcacctgg gcctgctggg
     5521 gctggagaga cgcagggatc gagacattgc ccgccaggcc atgtcgttca cccacgtcga
     5581 gcacctggcc gaacggaagc tggacgaact cagcgccggg gaacgacagc gggccctcct
     5641 tgcccgtgcc gtctgccagc agccccgaat catcgttctc gacgagccca ccgcatccct
     5701 cgatccggcc caccaggtcc atatcatgga tctcatggaa gggctccgga gggaagaggg
     5761 gatcaccgtc gtcatggttt cccacgatct gaaccttgcc gccttgtacg cggatcgcct
     5821 tctgcttttg aaggagggcc gggtcgtgag cctggggacc cctgccgaag tgcttgtttt
     5881 tgaaaccctg gagagagcct atggctgtgt tctcctagtg gacgagaatc ccctcaagaa
     5941 ggtgccgcgg gtaaccgtgg tgccggagaa cttgctcacc gggcacacga aatcctcgag
     6001 ggcaatggga aaaggcccgg ggggttctcc ataaaccgtt cgggggcgac gatccgagag
     6061 cgacacgccc gaaccccggc ggcctccggg ggggcaggtc ttggggcatc gtacggtgtc
     6121 cgaagacggt cgtcgtcccc accgccctcg atgccgcggg gagctcgttc aattcggagt
     6181 cgaggacttc ggctccggcc gggcgtctgt ttccgtactt cctcgccggg cacgatccga
     6241 ttgcgccgga aaccctcgag ttggacctcg gtccgatgcc gggtcaaaca ggagggccgt
     6301 gtcctcgagg tcttcgagat ccaggacggt ccggctcagc gcgttcacct tgtcctgccc
     6361 ccaagccggc agggccagca gccggaactt ggacacgagc gtttcctcgt ccacggggtt
     6421 ttcgggatcc ccccggggga aatcggtcct tgccgaaaag ggcccgaccg accgggtttt
     6481 gatctcgacg acggcgcccc accgggcggg atgaagagcg tcgagttcct catccggttc
     6541 cagatgcacc cggctcatga gggttcgaat ttcggaatcc aggaggttct cccgggtaaa
     6601 atcactcagg ccgcagcgcc cgcgcttcaa ggcaagagcc acacagaagg gcagactgaa
     6661 ccgggcgtcg aagaccgtcc ggggggcggg attccggatg atgttcaggg ccgtgcggta
     6721 ggttcggacg gtaacggaga ggacctcccg ggagtcgatt ccgtgctttg cggaaagatc
     6781 caggaccagg tccacggccg ggtgagtgtg gcgacacgaa gcatggatct tgaaggaatt
     6841 ctcctggatc ttgtacgggg gcgctcccag gccttcggtg atcttcgaca gatcgaaagc
     6901 cggggccatg gcccggcaaa atcccttttc cccctcgatg acggcctcgg cccccgtgaa
     6961 cccctctttg gccaacaggg ccgcaagcaa cccgttgtag gccgcttttc ccggatgcag
     7021 gtgcttggac atggccccgt cccttaggaa ttcccagagc ccggcagcct gggtgcctgc
     7081 gttgcccagg gcccacacct tttgccggcg gtccaatccc agcagttttc cggctgcggc
     7141 gcaggaaccg aaggtcccgc aggtgcccgt gttgtgccag aagaagtagt gggaagggct
     7201 cacggcctct cccaaccgga cggcaacctc atagcccgcc accacggcct cgatgagcct
     7261 ccgtccgtcc gcatgcacca tttccgccgc cgcaaaggcc gccgggagga tcgccgcccc
     7321 agcgtggagg atggagcccc ggtggacgtc gtccagttcg acgatgtgag acgcaatgcc
     7381 gtttgccagg gctgcgttca ggcacgaggt tttctccgga taaggaagaa gcgtggactg
     7441 gggggcacct ccctggtttc gcaggacggc cgcagccatc ttggcgggag cctcgagacc
     7501 gccgcgggcg gcggatccca gccagtccag gaaggccagc cgcgccgacc tcaccgtttc
     7561 cccgggaaga tcctcgtagc ggaccgaatc aatgaaatca gccagttttt cgctcaacgt
     7621 ggaagccatc ttctcccgtc ctcaaagctt gcgtgcggcc tgcacgatgt cttcggatat
     7681 cttgatcccc ttgggacaat gtcggggcaa ccgatcgaca aacagcttca acacctcggg
     7741 ggaaagctcg aggagccgaa gacgatgctc ccccagggcg tcccggggat cctgggccag
     7801 gcgcaaggct ttcaccaccc cctcgggctt ccccttcgcg gtctccccca agtccccgcg
     7861 acagatcccg caaaggatgc aggcggcgga ttcctcgagc tttgcggcgg tttcggggtc
     7921 cattcttccg tcgtagtccc ccctcaaaac cagccacggg acaacggcct tcaaggactc
     7981 gaagaggggc tccaggtcca ccaccaggtc ttttagcggg cggaagtggg gaaggggctc
     8041 gagggttacg gaatttcccc cggagacggc atttcgaacc agggtttgac atgccagggc
     8101 gggccgaccg tttatgagca ttgcgcacga gccgcagatg gcgctccggc aggaacggcg
     8161 aaaggaaagg ccggggtcct gctgccatgc cgacagcaga gcatccagca ccgtccgggt
     8221 tccgttcaca ctcaccgtga aggcctcgaa acgaggaccc gggtcccttt cgggagtgaa
     8281 ccgacagatt tcgagcgtca ctctcatttc agtagcccct tttctgaggt tcgtggcggg
     8341 tgacggcaac cgggccccga aagaactcgg gctccgcgtc ttttcgcctc acgaaggtgt
     8401 gatgcagcca gttctcgtca tccctccggg gatagtccac gcgaaaatgc gctccccggc
     8461 tttcggttcg cgccagcgcc ccgcgggcga tgagggttga gagctcgagg aggtggccca
     8521 cctcgaggta atccatcagg gcgtaattga agggctggat tccgccccgg ggagagatgc
     8581 tttcatagcg ctctttgagt tggcggagct tctcgaccgc gtcctcgagt tcgaccccgg
     8641 tacggaagac ccccacctgg ttggtcatga gggtcgccat ttcccggcga acggcgaaga
     8701 gatcgtcctg tttcgaagat gccggagggg gcgtggaaag cagggaggtc cagggttcca
     8761 gggatcggga aaccgtctcc cgggaaagag cgggccaggg ggcgttgcgg gccgactccg
     8821 cggctttctt ccccgcccgc cttccgaaca cgacggtttc gagaagggag ttgcccccca
     8881 gccgattggc gccgtgtacg ctgacattgg ccgtctctcc ggccgcgaga agccccggca
     8941 gggtggtcag gccccacagg tccgttctca cgcctcccat gctgtagtgc tgggccggct
     9001 cgatgggtat gggcaccgtc gccgggtcga ccccggcata tcgaatggca aggtccctca
     9061 cctggggcag ccgttttgcc accagggcct cccccaggtg ggtgagatcc agctgaacgg
     9121 aatggtcctc gaatccccgg ccttctcgaa tctcgttgaa aatggcccgg gccaccacgt
     9181 cccggggagc cagttccatc ttctcggggg cgtaacgggc catgaacctc tcgcccgtgt
     9241 tgttgcgaag atggcctcct tcaccccggg ccgcctcgga aatgagaacg ttggttccat
     9301 agagggttgt gggatgaaac tggacaaatt ccatgtccga gagcacggcc ccggcccggt
     9361 aggcgacggc catgccgtcc cccgtgttgg cgtgggcgtt ggtcgtcttg gaaaaggcac
     9421 ggccgtaccc gcccgttgcc aggacgacct gtttcgagcg aaagatctcg aggcgggcat
     9481 tggcaagatc gtaggcaagg gcccccgcaa cctgcccttc ttccaccagg agctccagga
     9541 ggtgccattc ggggtagatc tttacgcccc gcttcaagat gtgttcatac agggcgtgca
     9601 ggaccacgtg ccctgaaaaa tccgccacga aacacgcccg gggaaagccc gctcccccca
     9661 gggaacgctg ggcaatccgg ccgtcggccc tgcgagtcca aggcacgccc atcttctcga
     9721 gttctcggat caccgagggt ccgtcggaac aaaggacctc gatggcgtcc tggtctccca
     9781 ggtaatcgct tccctttacc gtgtcgaaaa tgtgggcatc gatgctgtcc tcttggctga
     9841 gaaccgcgtt gaatcccccc tgtgccgccc cgctatggga gcgggtcggg tagatcttgc
     9901 tcaggacggc cacatcgacc ccgtcctcga cggcaagggc ggccatgagg cccgccaagc
     9961 cccctcccac caccagcact tcgtgggtga tcatgaattt cactccttga aatagatttg
    10021 ccgctcgaag tagtgaattg tccccccggg ccaacccgat taatgcaccc aacttcttgt
    10081 gccgtcaatg gcggagactt ctgccgggga aatccgcccc gcgacttttt cgtcctttct
    10141 gcgatgactg cttgcttaga ccgagcgatt cggctggtac gaatcccgtt tcgtcattcc
    10201 cgggtatgcg ggaatccggg aagagcattc ctaccctccg gatgtccgcc ttcgcgggca
    10261 tgacgggctg atgctttccg aatcactcaa attacggctt gatttccttc cggcgatcct
    10321 caatgtagtg gtttgatctc tcggctcacg gatcgcggtt cacggatcga aaccgcggca
    10381 ggaaatgcgg attttccagg atcggaagaa ttggaggcgt tctcatggcg cagaccgaat
    10441 tcagggtgct ttctcccacg gcaattctcg gctacgggtt cccccgggaa tccttcgaga
    10501 ggggcatgga gaaaaggccg gacctcattg gagtcgatgc gggaagcacc gacccggggc
    10561 cctactacct gggcgcaggc aaatccttca ccagccgttc ggcggtcaag cgggacctcg
    10621 agttcatcct ggccgccgcc gtggaacgtt cggtgccgtg catcatcgga accgccgggg
    10681 gctgcggcgc ccgggaacac gtcgagtgga cggaatccat catccgggag atcgccgggc
    10741 agaaaggcta cacctttccc atggccgtca tccatgcgga tgtgagcaag gagacggtct
    10801 tggaggccat ggagcggcag cgggttcgcc cccttcctcc cgcacccgaa atcaccgtcg
    10861 aggacgtgcg acactgcgtg cggatcgtgg ctcaaatggg agcggaaccg gtcttgcggg
    10921 ccctggacac ggactgccgg gtgattctct gcggtcgctg ctacgacccc atcccgtttg
    10981 ccgccccggc cgtccgggcg ggattcgatc ccggccttgc cgttcacatg ggaaaggtcc
    11041 tggaatgcgc agccattgcg gccactcccg gcagcggctg cgactgcgtg atgggcaccc
    11101 tgtaccggga cggtttcgaa ctggaatccc tcaatcccca acggcggttc acccgtgttt
    11161 ccacggcggc gcacaccctc tacgagaaat ccgaccccta tcatctgccc ggccccggcg
    11221 gaaccctcga tctcgaggca acccgtttcg aggccctcga tggaggccgg gtgcgtgttt
    11281 cgggaacccg gttcgtcccc gcggaccctt acacggtcaa gctggaaggg gtgaagcgca
    11341 cgggatatcg atccatctgc atcgcgggaa tcagggaccc cattctcgtg ggtcggatcg
    11401 ataccgtcct cgaggccgtc aaggaggaag tccgaaagga cctggacttt acgggccccc
    11461 tgctcttcca ccaatacggg aagaacgggg tcatgggggc catggaacct gaaaaggatc
    11521 gagggtccca cgagctggga ttggtcatgg aagtcatcgc ccccgatcag gagcaggcga
    11581 acgccgtatg ttcctacctt cgcagcaccc tcctgcattt cggctacccg ggccgcatct
    11641 cgacggcggg aaacctggcc ctgctctaca gcccttcgga cattgcctgc ggcgaggtct
    11701 acgagttcag cctgtatcac ctcatggaag tggacgaccc ggaatccctc tttcccatct
    11761 gcgtgcgcga ggtgaaacca tgaagacggt gccccttacg gaactcgctg cggtcatccg
    11821 cagcaagaac gccgggccct acgaactgac catcgacatc cttttcaaga acgcccggca
    11881 ttaccggtat ttgaaggaaa tccacttttt caccgaggag ctttttgccc gactctacgg
    11941 ggtgggtgtg gagcaggtac tcaacctggt gtacttcgac ccggcctcgg cggtgaagtg
    12001 taccctggtt cgcccgatgg tttccggaac cccgggagac gccgatgtct acggcgccca
    12061 gcagcacgct cccgtcctgg gtctgcgggt ccccctcgag gaatggccgt agagtcggga
    12121 agctcctgcc cggcggggga aggaacagtg ccgggacctg ttcccaaagg agagttcccc
    12181 ttctccccca ccgggggatg gagctttctc ccctgggcgg acggggggcc gggggcaatg
    12241 ttgctgcatc aacgatccca cctcccgtcc gggacacccg ggcatcattc cccgaccagg
    12301 gtcacgacca ccttcctttg cctgcccttg atgtcgcatt cgaggtggaa gatctgctgc
    12361 caggtcccca ggacgagctt cccgtcgctc aagggcacgg tcaacccggg tcccaaaagg
    12421 gtcgcttgaa gatgagagtg cccgtttccg tcgtgccagg cctgttcatg gccgtagttg
    12481 cggctgggcg ggatcaagcg atccaggagc tcgggtaggt ccctttgaag acccggctca
    12541 aactcgatca ctcccagggc gcccgtactt ccgacgttga agacatgggc gattcccgtt
    12601 cgaatccccg aggcggcaac cacttgggcc accctgtccg tgaggtcgtg catgtcgccg
    12661 tgtccgctgg tggaaaccgt caaggttcgc tgatagacca ttttctttct ccacatcgag
    12721 gtgctcaaac actgtcgcga tcaacccctc gcccgaggaa ctcagggaga gggtggaacc
    12781 aagaagtgac tccccagagc gatcaaggcc cggtcgaggt cccgacccgc aaccacaacc
    12841 gtcatggaag acacggcaca actcagggcc aagggctgga tgccggacct gtgcaggctg
    12901 gtgaggagag cccccaccac cccgtagcgg tcgccgaaat gaggaccgtg gaggacaaag
    12961 gccgacacat cctcgtggca gacggcctgg acccgtgaaa gatcccggga gagaacgcga
    13021 cccacctgat ctcgccggtt gccgtcaagg cagagggcaa agtggagcat ctcctgcggg
    13081 ccgggctgac ccgtgaagaa aggcatgctc aggccatgga cctgcatatc ggccagggcg
    13141 tttcccaacc cggccaggcg agaggagggg acctcaagcc tccagaggtc gaggtcccgg
    13201 taccgaacgg cattgtaggt actgatgatc tgttcctcgt aagaacaggc aatttctttg
    13261 gccgcagggt tcttgtccgg gaatgccgca cgccagtcgt caagagaggg gtaagcagtg
    13321 aattcgaaga gatcaaacag gcctcggagg aatcgttcac tttgcccggc gggcaccaag
    13381 atgcagaggg aggagggaga cccggcaaaa cccgcgggct cgatcccctc ccgggcaagg
    13441 aatctcatca acgcccccgg aatatccggc ctctgaccgt agggaaacac cgtgaccagg
    13501 gcgacctccg acggcatccg agtgagaccc cgttctcccc gggcggcggt gagatcgagg
    13561 gaaacggcgg cgccgcgggc cggttccatg cacagggaca cctgggcggt ccgagcccca
    13621 cgacctgcat cgtgagtcag gaaggccaca ttgatccttg cggccgccag gcgggagcaa
    13681 aggagctccg gcgtggtctt atcgtcccgg tgggaatcga aaatgtgcgt acacccttcc
    13741 aggaccttca ggccgttgag tcggatcctg ctcatggcgg tgaactcccg gcacaggggt
    13801 cggattcttc ttgaatccgt gagaacaaaa gcatcgcacg tctattggtt ttactgttac
    13861 catgctttga tggttttcgt aagaacccaa gcgcctgccg catccccgaa gatgaccggt
    13921 gaacgcgttt gcggcccaag ggcaggggta tggtatgaaa accggtctct ccagccgggc
    13981 aagaggcatg agattttgct gaaaaatgga agcagagggt ctctttctca atcgatagcc
    14041 cccagaaggg gtttttacga attcctcaaa tatcgtacat atctcgggtt tactggtaac
    14101 atgttgtaat gtaaggccaa tcatgttacc tccccacctc atcgcaaccc cctcgggagg
    14161 ggacgcgatc cgtgaagcaa tccatcatcg cacagtcgga caaaaagaaa agctcgaagt
    14221 catggcgccc aaccacagca ttccccgggg cgtcgggtct gcaaatcaaa gggacccatc
    14281 ggggctcgga accggaccct tgcccccgaa tcggccccag gcctcctact tggtggtcga
    14341 acaggggccg ggacagccac ggcggacggg gagcggaccc aaggctggtt ttttgcgccg
    14401 ccccggggtc cgcaccggtc gggatcctac ggcgcccttg tttcggggtt cgatgaaacg
    14461 ggaccctgtt ttggacgcag atcgggctct cgtgtccccg gcgcagccct aagaccatcg
    14521 ggtgaagacc accactccgt tctgaccgcc aaacccgaag gagttggaaa ttgccgccat
    14581 gacttgcgct tcgcgggctt cgttggccac gatgtggaga tcacattcgg gatcccttgt
    14641 atggtagttg atggtcgggg ggatcacccc gcggtgcagg gtgagcaccg ttgccgcggc
    14701 ttcgatggcc ccggccgccc ccaaagtgtg gccgatcatg gacttgacgg acgtaaccgg
    14761 caccgtcttt gcccgctccc cgaaggcctt ctctatggcc ttggcttcga ccttgtcgtt
    14821 gacttttgtc gaggtcccgt gggcgttgat gtgcccgatt tcttccatgt tgagccccgc
    14881 gtcccggacg gcgttcgcca tggccgcagc ggcggccgtt ccttccggtt ccggaatggc
    14941 gatgttgtag gcatcgcaac tcatcccaaa gcctttgagc acggccagga tatccgccct
    15001 gcggtccctg gcatgggata gggtctccag caccaggacc ccggcaccct ccccgatgac
    15061 gaacccgtcc cggtcggcat cgaagggcct gctggcggtt ccgggatcgt cgtttcgctg
    15121 ggacaaaacg cccatgcacg catacccgtc cacgacatac ggggtgatgg cggactccgc
    15181 tccccccgcc aggaccacat ccgccaggcc ctgccggaga atccccaggg cgatcccgat
    15241 ggcgtgggct cctgtggcgc atgccgtgga aacccccagg ttgggacccc ggattcccaa
    15301 ggcaatcccg acgttggagg cgggcatgtt cgggatcacc ttggggatgg ccagcggatt
    15361 gacgaatccc ggacctttct ccttgaaccg gtcgtattgc tgttccaggt tgtagtaatc
    15421 cccggccgca gaacccacca cgcaccccat gcgatgggga tcttccctct ccaggtccag
    15481 cccggcatcc cgaacggcgg cgaaagcggc acagcccgcg agctgcgaga cccgggccat
    15541 tcgccggacc tgcttgaagt tgaggaactt ttcgggttcg aacggaacga cttccgatgc
    15601 gatcctgctc ttgtgccggg acgcatcgaa ggcccgaatc cccttgattc ccgaagttcc
    15661 ggacaccagg gagttccaga aggtttcccg gtcgcatccg atggaagaaa aaacgccgat
    15721 tcccgtcaca accacttgga tcataaggac ctgtcctcga aaaccgaaat ttgaaaggat
    15781 tgcatcgccc aaagcccttg tccgcatgca ggtccccatc ctaacaccac tcccgcagtg
    15841 aaaccaaccc cctccccgga tcggaatttg cccggcggaa gggaagatcg gattccgacg
    15901 ggttttccaa cggtttttcg ccccggggat gacttgttca tgacaggagg gacgaagttg
    15961 ggtatgaatc tcctgaaaaa ccttgggaac cggtccgctc tcgtgcaggg ccaggaacac
    16021 tcgaggtgcg gacccgggaa accgcgggca ccggcggggt cggccaaacc ggggaacctt
    16081 gaaaacaggg ggtgaagcca tggaatgcag gattacgatt ctttgcgaga acacggtcca
    16141 gggaatgggg ttttggggcg agcacgggtt tgccgccctg atcgaaacac cgggacaagc
    16201 cgtcctattt gacaccggcc agggtttcgg cctggttcct aacgcgctgc gactcaagaa
    16261 agacctgggc ggggtgtcga aggtggtctt gagccacggc cacctggatc acgtgggagg
    16321 gcttcttgcc ttcctgggag tcaaaggccc ttgccccgtg gtggctcacc cggatctttt
    16381 caccgaaaga ttctggctca tgcccgcccc cggcggcggg gaggaaccgg tatccatcgg
    16441 catgccctgg ccccggtcgt acctcacgac ccggggcgcc cagtttcaat ggtgccggga
    16501 tttcgcccaa gtcgccccca acgtcttcgt cacgggcgag gtaccccgaa agaccctctt
    16561 cgagctgggg tctcccaagt ttgtggtgaa ccacaacggg ggattcatcc cggatccttt
    16621 tctcgacgat ttttccatgg tcctcaagac ctcgatgggc ctggtgattg tcctgggctg
    16681 cgcccacgcc ggaatgatca acgtgctcca acacgccgtg gaaaagacgg gagaagaacg
    16741 aatttacgcc gttctcggag ggactcacct gggcctctcg ccgcagcccc agttggatcc
    16801 gaccatcgag gccctcaaga agttcgacat cgggatgctt gccgtcagcc actgcaccgg
    16861 gcaggctccc gtggcccgcc tcgcctcgga attcgccgac aggttcgcct tcgggcgcgt
    16921 aggcttcgta ctcgaggtct aggaaacccg gcaaacctgc ccgtatcctg gctcgcccgg
    16981 ccgggggcgg ccgaaagacc cttcccggga ttgtatcgag ttgcccacgg gtttgcggcc
    17041 ggtcccccgg gagatccgtc gaggggaacc gtagagacaa ggctgtaccc atgaagcgga
    17101 agacaaagtc cctactggtc ctggcaggcc ttttcccggt gttctgggga tggacgcccg
    17161 aggggcgcgg tttcccgtcg gccggggctg cgagaatcgc cgggtgcccg gattctccca
    17221 actgcgtctc gagccgggat cctccggggc gccgttggat tgcccccctg ggcctccggg
    17281 gctccccgga agaagccttc cgatgtctga aagaaatcct ccttcaagga ccgcgcacaa
    17341 ccctcgtggt cgaggaaggg ggctaccttc acgcagaatc ccggaccgcc ctgggatttg
    17401 tggacgacgt cgagttcgag ttcgacccgg aaagccgcag cattctcttg cgttctgcgt
    17461 cccgaacggg ctactgggac ctgggggtga accgaaggcg actcgaggcg ctgagggccg
    17521 ccttcgagtc caaatgcagg caggggggaa actcccggtc ggcaagggac cgcacctctt
    17581 tgcccgggaa cggtcggtag gaaaaagacc cggccgtaat gggccgggcc cggaggttca
    17641 tccttcctcc cccggcccga tgaggggaag gaccttgtcc acccggaaga acacaacgaa
    17701 gcgatcccct tctttctcct gcgactcggg ggtggaatgc cttcggcgca gagtatgaac
    17761 gagatcgggg tccgtttctt cccggatctt ggtcaggtga agcctttttc ccttgtaacc
    17821 cggtccctct tcccggaaca gataggccgc acggggattg gactgcaggt tgaagtgggt
    17881 gaggcgatcc ggcatgagga aggccaggct cccgtcgtcc atggcatggg gccgtgaata
    17941 cacggccgag ttgacccgcc ccgtaccgtc cgccgtcgac aacaccccaa aacctttccg
    18001 gttctcgaaa tattccttca ggtccattgc cgtcccctcc ggttttgcga atcctaccat
    18061 ccgcctctct tggggttctt ttcccatggg ttgattccaa aaacctgctc ccgggcgcct
    18121 gcggagcccg attccctctc caattccgtc cgtggtcttc cggtctccag actcaattcc
    18181 tgagacacga ttggcatgag gggagacctc cggggccccg ggccagttcc ccaagagcgt
    18241 tgcgcacggc ctcttccatg gaaaggagcc ggatcggaat gagttctcgg attgcatggt
    18301 cccggcagac gacctcgttt ttgagtccct cgaccagggg catgaccact cccgccggaa
    18361 ccgaagaaac cagggtgatc cagtaggagg aaaggtgggg ggtcaggacc gggacggtga
    18421 agatccagcg gcggaggtgc cgaacccgcg cataggtctg catcagttgg cgatacgtga
    18481 ggatttcggc cccaccgatg tcaaaggtcc ttcctgccgt tttgggttca tccaggcatc
    18541 cttccaggta ctcgatgacg ttttccacgg caatgggctg gcagcgggta tcgatccagc
    18601 ggggacaaat catgacgggg agacgttcca ccaggtggcg cagcatttcg aagggggccc
    18661 ccccggcccc gatgatgttg gcagccctca gcacggtgac cggaactcgg ctcgattgga
    18721 gaagtcgggc cacctcctgt cggctccgca agtgctcgga gagattgtcc gccacttccc
    18781 ccagtccgcc caggtaaacg atgcgccgga ctcccgattc ctcggcggcc cgggcgaagt
    18841 tacgggcggc atcgaggtcc ttccgaatgt agtctttgta tcgaaaggcg ctccggcccc
    18901 ccatggaatg gaccaggtag taggccgtat gggtcccttc catggccccc cggaaggttt
    18961 cgggttgcag caggtccccc cggatcaccc ggggttgggt gccgcaaaag ccccgggtgg
    19021 gaaaatgctc cgccgagcga accaggcagc gggtgggaac gccccgggac gccagggctc
    19081 tcaagagtcg gccgccaatg aatcccgagg cgcccgtcaa aaagacttcg gatgaaccgt
    19141 cgttcatatt gcaaccctcc cgaccggaaa atcatcccct catgggatga gcggtcagta
    19201 taacagagga acccaagagg gcaatcgacg ttccttggac ccgatggaaa tttagcaaaa
    19261 cagtccttga ctcctcgacg cattcttcta caattcgacg ccacgttcat tttgtgaatc
    19321 atcatacagt ccgcgaggtc caaggatgga agaaagaaag ctctccaggc gagaacgaga
    19381 gagactggcc cagcgtcggg agatgcttgc cgccgccctc gatcttttct ccgacaaggg
    19441 gtaccacaat gtctccatgc accagattgc cgagaaggcc gagtttgcca tcggaaccct
    19501 ttaccggttt ttcaaaaaca aggaagacct gtaccgagct ctcatccgag agcagtccga
    19561 caagttccat gaggccctga cccgggccat cgaggaaccg ggcgacgagg tcgagaagct
    19621 gcgaaggtac gtcagggcca agagcgaggt atttcgtgcc aatgttccca ccattcgcct
    19681 ctattttgcg gaaacccacg gagcgagatt caacatcatg gccggggtcg attccgagat
    19741 ccgggagcgt taccggcaat tccagcaaac tcttgcagga gttttcgaga gcggaatgga
    19801 gagggaacgg ttccaaagaa ttgcgacccc gtattccctg gccgtagccc tcaacagcat
    19861 caccaatgcg tttctctctc tctggcttga agacccggaa caccatccct atccggaaga
    19921 cccggacacc attttaaaca ttcttttcaa aggtctgatg aatgaatgat cgtgccgtgc
    19981 tccgtgttat gaacgtggaa tactttcctt tcaatcggag attttggagg tctcggatgc
    20041 aacgaaacga aaccgtgaat ggaccgcgta cgatccttgt ggtttggatg gttctgttgg
    20101 tcggcctcct tgcgggcggc tgcgaccgcc gacagcaggc cccgccgccc cctgccgtgc
    20161 cggaagtggt tgtggagacg gtccggaccc atcgcatcgt gctcaccacg gaattgcccg
    20221 gtcgcaccgc gccttatctc gtggcggaga tccgacccca ggtaaacggc ctcatccaga
    20281 agcgcctgtt caaggaaggc tccgacgtca aggtcgggca ggtgctctac cagatcgagc
    20341 ccgccccctt ccaggcggcc ctggacaatg ccaaggcggg ccttgcccgg gccgaagcca
    20401 atctgcccgc ggctcggctg agggccgagc ggtacaagga gcttcttgcc gacaaggcgg
    20461 taagccgcca ggactacgac gacgcggcgg cggctctgaa acaggcggag gcggatgtgg
    20521 aatactggaa ggcttcggtg gaaaccgccc ggatcaacct gggttatacc cgggtcaccg
    20581 cccccatttc cgggcgcatc ggacggtcca acgtgaccga cggggccttg gtcacggccc
    20641 atcagcccct ggccctggcg accatccagc agttggatcc catttacgcc gacgtgcccc
    20701 agtccaccac cgacctgttg cggctgaggc gccgcctcga ggacggccgc ctcaacgaga
    20761 acgggaccga ccagaggaag gttcggctca tcctggaaga cggatcgagc tacccccggg
    20821 aagggaccct ccaatttcga gaggtcaccg tggatcccag cacgggctcc gtcatcctgc
    20881 gcatcgtctt tcccaatccc gaaggagtgc tcctgccggg catgttcgtt cgggccgtgg
    20941 tgcaggaagg gatcaatcag caggccatcc tcattcccca gcagacggtg gtgcgcgacc
    21001 ccaaggggaa tccgtcggtg ctgatcgtga attccgagga caaggtggaa cagcggatga
    21061 tcaccctgga tcgcgacatc ggagatcaat ggctcgtgtc ctcgggtctt gcccccggcg
    21121 atcgaataat cctcgagggc atgcagaagg ttcggcccgg cgtccccgta aaggtcgtcg
    21181 ccaccggagc cggggaaccg gaacccggga aaccggggca gccttctcaa cccgctgcca
    21241 aatccaattg acggaggcac acgatgctat cgagattctt cctggatcgc ccggtattcg
    21301 cgtgggtcat cgccatcatc atgatggtgg cgggcggtct ggccatctac aacctgccca
    21361 tctcccagta cccgcccatc gctcctccgt ccattgccat cgattcgttt tacgccgggg
    21421 cttcggcgga aacggtggaa aacagcgtca cccagatcat cgaacagaaa atgaccgggt
    21481 tcgacaagat gctgtacctg aaggccacca gcgattcctc gggttcatcc cgaatcgagc
    21541 tgacgttcgt gccggggacc gatccggacc tggcctgggc tcaagtccag aacaaactcc
    21601 agctggccat ggcgaacctg cccgaagtgg tccagaaatc gggggtgaag gtgagcaagt
    21661 ccacccggaa ctggctgatt ctcgtgggtc tgatttccga ggacggcagc atggacggaa
    21721 acgacctgcg ggactatgcc aagtccaacc tcgagaaggt gctctcgagg gtgcccgggg
    21781 tgggggaagt cgaggccttc gggacggaat acgccatgag aatttgggta gacccggaca
    21841 agctcatcaa ttaccagatg acggtggaag acgtcatggc ggccctcgag gcttacaacg
    21901 tggagatctc cgcaggccag ttcggggggg cccccgcggt ggacggccag cgactgaacg
    21961 cctccatcat cgttcagagc ctgctcagga cccccgacga attcgcctcg atccccatac
    22021 ggctgaaccc cgacggctcc accgtgcgga ttcgggacgt agcccgggtc gagctgggaa
    22081 ccgagaccta tgatgtcgtt gcctaccaca acggcaaacc cgcggcggcc ttggccatcc
    22141 ggcaggctgc cggggccaac gccatcgaca cggccgatgc catcaaggca aaaatggagg
    22201 aaatgagccg gtattttccc ccgggcatga aggtcgtgta cccgtacgac accactcctt
    22261 ttgtcaaggt cgccatcaag gaggtggtga aaaccctctt cgaggccatc gtgctggttt
    22321 tcctggtcat gtacgtgttc atgggaagca tccgggccac cttcattccc accatcgcgg
    22381 tgcccgtggt gatcctgggg accttcggag tccttggatg gctcgggttc tccatcaaca
    22441 tgctgaccat gttcgccatg gtgctcgcca tcggtttgct ggtggacgac gccatcgtgg
    22501 tggtggaaaa cgtggaacgg atcatggccg aggaagggct ctcgccccgg gaagccaccc
    22561 gcaaatccat ggacgagatc accagcgccc tcatcggcat cggcctggtg ctctcggccg
    22621 tattcggccc catggccttt ttcggcggtt ccaccggcgt catctaccgc cagttttccg
    22681 tgaccatcat ctcgtccatg ctcctgtcgg tggtggtggc cctgatcctc accccggtcc
    22741 tatgcgcctc gctcctcaaa cccgttccca aggggcacca gccctccgac ggtgcggtct
    22801 ttttcctgcg acctttcttt cgatggttcg accgcttctt cctgggctcc cggaggttgt
    22861 acctgagggt ggtcggccac gctttggcca agaaattccg ctacctcctg gttttcctct
    22921 tgattgtggc cgccttggga tacctctttc gaaagatgcc caccgcctac ctgcccgacg
    22981 aagaccaggg aaccatgtat gtccaggcca tgcttccggc aaactcgacc ctcgaacaga
    23041 cccagcaggt catggacggg gtgagggagt attttctcga gaaggaaaag gacgcggtgg
    23101 agaccgtcct gacggttgcc ggtattgcct ttgccgggcg gggacagaac atggccatcg
    23161 ccttcgtgcg gctcaaagac tgggagcttc gccaacgccc ggacttgaaa gtgggcgccg
    23221 tggcggggag ggccatgaag gccttctccc agatccgaaa cgccatcgtg gtggcgttcc
    23281 cgcccccggc ggtcgtggaa ctgggcacgg ccaaggggtt tgattttcag ttgctggacc
    23341 ggggggggct gggtcacgaa gccctgatgc aggcccgcaa ccagctcctg ggcatggcgg
    23401 cggccgatcc ccgactccag gccgtacggc ccaacggcat ggaagacgtt ccccagtacc
    23461 gcatcgacgt ggactgggaa aaggccggag ccctgggggt ccccatcact tccattcacc
    23521 ggaccatttc caccgccttc ggcagctcct acatcaacga cttcatccag tcggggcggg
    23581 tcaaacgggt ctacgtccag gcggacgccc ccttccgcat gctgcccaag cacctggggg
    23641 agctctacgt gcgaaactca tcggggaaaa tggtcccctt cagctccttc gcctcggggc
    23701 attggttttc gggctctccc acgctcgaac gctacaacgg ctttccgtcc atcaacatct
    23761 ggggggaggc ggccgctggg agaagttccg gtacggccat gaaggccatg gaagagatcg
    23821 cctcccaact gccccaaggg ttcggctacg actggaccgg tctttccttc caggagcggc
    23881 tggcgggcgc ccaggcaccg gtgctctacg ctttctcgat cctggtcatc tttttgtgcc
    23941 tggcggcttt gtacgagagc tggcccatcc ccatttccat cctcatggcc ctgcctctgg
    24001 gggccatcgg cggcgtcatc ggctcgaccc tcaatcggct gcccaacgac gtctatttcc
    24061 agatcgggtt gctcaccacc ctgggtctga ccaccaagaa cgccatcctc atcgtccagt
    24121 tcgccgaggc caaggtggaa gaaggcatgg acctcattca cgccaccctg gaaggagccc
    24181 ggttgaggct ccggcccatc gtcatgacct ccctggcctt cgggttcggg gtcctgcccc
    24241 tggcccttgc cgggggggcc ggagcgggag cccagagggc catcgggatc ggcgtccttg
    24301 ggggggtggt ttccgccacc ttcctggtga ctcttttcgc cccgctcttc tacgtgatca
    24361 tcaccaagat gtcccggaaa cacagaaggc gtgtggccgc caaaacagta gaggcaactc
    24421 catcggggga tcaaggacat gaatagaaac cggctccttc tactgggtgt catcgtcacc
    24481 tttctgggcg gctgcacgcc ggccccgaaa tacacaaggc cgggagcccc cattcccggc
    24541 gactggcccg acggggctgc ttatcaggac agccgggccg tccccgacgc cccggcgccc
    24601 acggcgctgt cgtggcgcga gtttttcggc gacccccggc tccaaaagat catggaaacg
    24661 gccctgagaa acaaccggga cctgcgcctg gccgtgttga acgtggagag ggcccgggcc
    24721 atgtacggcg ttcagcgcgc cgagctcttc ccggtgatca atgccgtggg ggccgggagc
    24781 aaatatcgcc tgcctgcgga tctttcgagc agcggaaaat ccacgacctc cgagcggtat
    24841 gacgtcaatg tggggattgc cgcctgggag atcgatcttt tcgggcggat tcgcagtctc
    24901 aaggaccggg ccctcgagga gtattttgcc accgaacagg cccgccgtgc cgcacagatc
    24961 ctgctgctct ccgaaatcgc ccgtgcctac ctggccttgg ccgccgaccg tgagagcctt
    25021 cagttggccc aattcaccct ggaatcccag caggaaggct acgacttgat tcgacgccga
    25081 tatgaaaagg ggatggcgac ggaactggac cttcgccagg aacagacccg ggtggaagcg
    25141 gcccgggtgg acgtggccct cttcacgcgg ttgatcgccc agggcgaaaa cgccctggat
    25201 ctgctggccg ggtctcccgt gcccaaggac ctgtttccgg ccaacctgga agacgtcggt
    25261 ccccaccccg aggtagctgc cgggatttca tccgaggtac tcctaaaccg cccggacatc
    25321 ctggaggcgg aacaccggct caaagccgtc aatgccgcca tcggggccgc tcgagccgcc
    25381 ctcttccccc gcatttccct gaccaccgcc gtcgggaccg caagcagcga tctcacgggc
    25441 cttttccagg ccggatcggc cgcgtggacc tacgcgcccc agatcgtcct gccgattttc
    25501 gacgcccggg cctgggcggc cctggaggcc gtgaaggcgg aaagaaagat cgtgctgacc
    25561 cagtacgaaa aggccatcca aacggctttc cgcgaggtgg ccgataccct ggcggtaaag
    25621 ggaaccatcg acgaccagct gacctcccag gaagccctgg tttttgccag cgccgagacc
    25681 taccgcctct ccaatgcccg gtacatgaag ggaatcgaca gcttcctggg cgtcctggtg
    25741 gcccagcgct ccctctatgc ggcccagttg cgcctcatcg ccatccggta tgaaaggctt
    25801 gccaatcagg tgaggctgta cgcagtgctg ggaggcggcg ccgacccggc cgtgccccgc
    25861 gctgaggcgc cggggcgcaa cgcggttgac taaaggaccg accaaggctc ccttattccc
    25921 gcttcggggg agagggtcgg ggtatctcca gggccgatct ccccttcacc ctctcccgca
    25981 gggagagggt gaagggtcaa cccgctgagg tccggttcat gggcattgcc tcggatctgg
    26041 taatcatcgt cgtcgcggct cttgtgggcg gcatcgtcgc tcacaagctc aaacagcccc
    26101 ccatcctggg ctacatcatg gcggggatgg ccgtaggacc ctactcggcg ggagccaccc
    26161 tgtccgacat tcacgacatc gaactcctgg cagagatcgg ggtcgccctc ctgctgttcg
    26221 ccctgggcct ggaattctcc ctcaaggaat tgaagccggt gcgcagaatc gccctggtgg
    26281 gaactcccat ccagatggtt ctcaccatgg gctacggctt cctcgtgggt caatggctgg
    26341 ggtgggagcc ggtcccttcc ctctggctgg gggccctcat ttccctgtcc agtaccatgg
    26401 ttcttctgaa aaccctcatg aaccaggggt ggctgggcac cctctcgagc cgggtcatga
    26461 tcggcatgct catcgtccag gacctggccg tcgtgcccct catgatcctg ctgccgcagt
    26521 tgagcaaccc caaggcggga atccccctgc tgggccttgc ggccttgaag gccgccgcct
    26581 ttcttttctt catggttctt ttgggctcga ggctgcttcc ccgcctcatg gcctacatcg
    26641 ccgggtggaa ttcacgggag ctgtttctcc ttgccatcac cgccatcggc ctgggagtgg
    26701 ggtacgccac ctacctttcg gggctttctt tcgccttcgg ggctttcgtc gccgggatgg
    26761 tcctgagcga atccgattac agtcaccagg cattggccga catcatcccc ttgagggaca
    26821 ttttcggcct gctgtttttc acatcggttg ggatgttgct ggatccctcc ttcctggtat
    26881 ctcattggaa caccattctg ctgctggtgg tgctcacctc cgttggaaaa gggttcctct
    26941 ttgccgccct gggtcacgcg ttcggatacc gcaacgtcat ccccctggcc ctgggactgg
    27001 gactcttcca gatcggcgag ttttcttttg tcctggcccg gctgggggtt tccaccggat
    27061 ccatctcgaa cgaagcgtat tccctggtcc tcagcacggc ggtcacaacc atggtcctga
    27121 ccccgttcgt gtcgggactc accgctcccc tgtactccct tcgcaagaga cgattcaagc
    27181 acgaacccgt tcagaccatc aaccttcccc agtcgggact ctacgaccac atcgtcatcg
    27241 caggcggcgg gcgggtgggt ctgcacgtgg cgcgggtttt gcgcaccctg ggccacccgt
    27301 ttgccctcat cgatctcaac taccggcagg tggaggaggc caagagggac gggttccccg
    27361 tcatctacgg cgacgcaagc caggaagtgg tcctggaagc tgcgcacctt gacaaagcgc
    27421 gactgctcct catcaccatc cccgccgtgg tcgtctctca ggccattgtg gacctcgccc
    27481 gacaaatcaa gccgaccctg agcattgtgg ctcgaaccga gggcatcgag cagatgaagg
    27541 ccctccatgc caagggggtc gccgaactgg tgctgcccga gtttgaagcg ggcctcgagc
    27601 tcacccggca ggccctccgc cacctgcacg tcccggcgtc ccagatcctg cgatacaccg
    27661 acaccgtacg tcgagacctc tacggccccc tctatgagaa cgaccatgac catgataaac
    27721 ttagttttcc ccggagtgcc ggaactttgc tcgaactgaa ctggtttgcc ctggagccgg
    27781 acagcttcct gatcggccga accatcggcg aactggagat ccgcactcgc acgggggtct
    27841 cggtggtcgg cgtcatgcgg gaccgggatc ttcaccccaa tcccggtccc gattttcgtt
    27901 tcggctcggg ggattttctg gccgtcatcg gcaagccgga gcagttcgac gcgttcgaac
    27961 gatttgccgg ggcgggctcc ggccctgatc cggggtaacc gggcgtgcgg gcattccaca
    28021 ggcaggaaag gatccaacca tgagagtatc gagaatccca agacgactga cgacctctgc
    28081 tgctttcatc cttctcatga cggggcttgt ttccccggga ctggcttcgg tcaagcttgt
    28141 gaagggacag acggtctatg tgccggccta ttcccacatc tattacgggg accgggaact
    28201 gcctttctac ctgacggtta ccctgagcat tcgcaatacg gacccgaccc atcccatcac
    28261 cctggtttcc gtggactatt tcgactcgga cggaaagagg ctccagagtc acctggcaaa
    28321 ggaactgaag ctgaatccca tgtcgtccac ccgcttcgtg gtgaaggaat cggacaagag
    28381 cggagggtcg ggagccagct tcctggtgac gtggaaatcg gacaagaagg tctgtgaacc
    28441 cgtgatcgag acggtcatga tcagcacgtc cacccagcag ggaatttcct tttcttcccg
    28501 aggaaagccc gtcaaggaac tggaggattg agggtcgaag aaccggaatg cggggcgccc
    28561 gggattgcgc cctttgccct ccacccggtt ccgggcaacc ccgtttcctg cgagtgacgg
    28621 gtttgcattc cttggcggga ggaagaatga accgtaacct gaagattctc gtggcgggat
    28681 acgccatcgg gttcccactg ggcgggcaat tgtggatgat gctccagtat gtcctgggtc
    28741 tcaagcgact gggtcatacg gtattgttcc tggaagacac ctccgactgg tcctatccct
    28801 atgacccggt gcggcgttcc tacgatttgg gatcctccta cggccgcagg gtcctggacg
    28861 attttttcgc ccgctgcggt ctgaagggcc agtgggtcta caacgcgccc atcgagaacg
    28921 ccgtctacgg catgtcccgg gaggacctgg accgcttctg ctcccaagcg gatctctttt
    28981 tgaacatctc ggggatcact ccccttcggg aagaatacct gcggtgcagg gtcaaagcca
    29041 tcatcgacac ggaccccgtt ttcaaccaga taaaaatcag caacgacgag aaaagccggg
    29101 agtatttcca gtcccatgat ttttgtttca ccttcggctg gaaccttccc gacggagtgg
    29161 cgggggtccc ccattcgggg atcgactgga aaagcacgct tcctcccatc ctgccggatc
    29221 tgtgggttcc tctcgagact ccgggcggcg ccttcaccac cgtaggttcc tgggactccc
    29281 ggggacggga tgtcctgttg aacggtcgag tcctttcctg gcggaagcgc cccaagtacc
    29341 atcaactcct ggacctgccc cggcacctgc ccggcgtcga cctggaactg gccttcctgg
    29401 gaattgaaga cgacgcaaag ctctttgcgg agcatggatg ggttatccgg gatgccgtgg
    29461 aggtttcggc ggacccgtgg cgttaccggg attacctgcg gaattcccgg ggagaattca
    29521 ccgtggccaa ggatcagaac gtgatcactc ggagcgggtg gttctcggac cgcagcgcat
    29581 cctaccttgc agcgggccgt cccgtgatta ccgaggacac cggcttcggg agctacctcc
    29641 ccgtaggcga gggcctgttc acgtacaaga ccctggagga ggccgtccag tgcatccggg
    29701 ccgtggaggc ggacccggtt cggcactttc gggcagcacg gcgcatcgcc caggaacact
    29761 tcgatgcacc caagatcctc tcccggatcc tcgcggatgc gggacttgct tgagacccac
    29821 ccgcaagaga gaagggcccc accggcatcc aaagttcccg ctcctccggg cggaggttgc
    29881 ctccggcccg gtcggcctcc gggaatccat ggctcaggtt cggggaaaga ctttgcaaag
    29941 agccgagggg tacccattta ttcagaacaa ccgtctcata cgaaattgca ctcaagtcga
    30001 aactgcccac ccgcaggatt cagccctttg ctcgtaatct acactcagca tgttagggcg
    30061 accttgagcg caacttggta tcatatccca aagccctgat tgactgctat tcccggctga
    30121 tcgctcgagc cgagaaaacc ttcgtcccgg ggaagccccg ggcaaggtca accaaagggg
    30181 acgccgtgga atcccttccc agccaaagac tgctctccct cgacgtgttc cgaggcgccg
    30241 ccatcgtggg catgatcctg gtcaacaacc cgggcaggtg gcgggtcgtc tattcccaat
    30301 tgcagcatgc cccctggaac ggctggacct tcaccgacat gatcttcccc ttctttctct
    30361 tcagcgtggg gatttccatc accttttcct tcaccaggag gatcgagcag ggtcatgccc
    30421 gttccaccct ggttttccag gccatgaaac ggacggtcct gctctttcta ctggggatct
    30481 tcctcagcaa cgctccccac tttcacatgg acaatcttcg gataccgggg gtcctgcagc
    30541 gcattgccct ttgctatttc atctcatccg tcctctttct aggatcggga tggaaaggcc
    30601 aagcctcgtg gatggcggcc ttactcctgg cctactggct catgacggct ctcatccccg
    30661 ttcccggcat tggagccggg gtctacgaac cgggcagaaa ctttgccgcc tacgtggatt
    30721 ccatcttcct ggagaaccac atgtggtccg cctaccgaag ttgggatccg gagggcatcg
    30781 tgagtaccct tcctgccgtt tcatccacgc ttttcggggt cctggcgggt cactgcctga
    30841 gaacggctca ctccatcccc cgcaagaccc tgggaatgct cgccgccggt gtcctttttc
    30901 ttctcctggg cttggccctc gatcccctgc ttcccatcaa caagagtctc tggaccagca
    30961 cctactgcgt cttcatgacg ggttgggcct tgctgtgttt cggcgtattt tattggctca
    31021 tcgacggcaa agggtaccgc aagtgggcgt ttcccttcgt ggtattcgga acgaacgcca
    31081 ttttcgcgta cggcttatcc aagctcctgg acgatctctt gaaactcgtc accattgccc
    31141 cccagggagg aggtccccgg attcggctca aacactgtat ctttgacttt ctcaacgccc
    31201 tggcgggcac ttccaagctt gcttccctcc tgtatgccgt cctgaacgtt ttggcggtct
    31261 tcctggtggt ctacggtttg tatagaaaac gttggtttct caagctgtaa cccgggacga
    31321 tccttggacc tcgggggagg ttgcttttgg cccaaggtcc tctgccgcag gcagagaatt
    31381 cggaaaagga ctccacggga aagccttccc cgggagaacc ggctgtcgtt tggatccgag
    31441 ttgagcgatt ccgagagcat cagcccgtca ggcccgccac ggccggcatc caacgggtac
    31501 gggcgctgtc cggggcccgc ctgcgctgga atcatccatg ccgcagggtg cactccgttt
    31561 gcacgaaagt cctgccttcg taggaatgag gaaatgggat tcgtgcaggc caaatcgccc
    31621 cacgtgggtt cgagatcatt gcctacggcg aagggccatg gccgtgtcgg gcgaaagaac
    31681 ggttgccgac cgggtgcggg aatggaggat gaaatccggg cgaggccgcc ccgaaggcgg
    31741 cctcgcccgg gtatggttcg agtgttgaag gacgacgcgg aatgatagtc ccccccggtc
    31801 cccattcccc tcccgccggg gaagagaaag ttttcccctt ccctcgatgg gcggaggccg
    31861 gggaagggtg aaggacctgc tcaggggaaa accctcagcg atcgaacagt tcttggagcc
    31921 ggtcggcctt gggcgtttcc tggagggcga gcatcaggag gatcctcgcc ttctggggag
    31981 gcaggttgtc ggcaaaaacc gccccggcct ctttcagggt cttgccgcct ccctggaagc
    32041 cgtaaacggg caggaccctc ccgttgggga ctcgggtcga aatgaccacg ggcacgcctt
    32101 tgcccatcgc ttctttgatg gcctcgaaca tgggcacgtt gacgttgccc caccccaggg
    32161 cctggatgac gatccccttg gcacctgcgt ccaccgccgc tttgaccagc ttcccatcgg
    32221 caccgccgta catggtgacg atctccacgt atgggagggt cgccttggtc agggggatat
    32281 gctgcctgcg caggggcgat cggtagaaga tcacccggtc gttgtccgcc gttcccagaa
    32341 acccgaagtc gccggacttg aaggtttcca cgtccgaggt atgggtcttg acgacttcgc
    32401 gggcggcatt gatctggttg ttgagaacaa tcatggatcc cttgcccttg gcgtcgggcg
    32461 acacgcagac cctggcggcg ttcaaaaggt ttctcggacc gtcgaagtct ttttccgagg
    32521 cattccgctg ggccccgatg aggacgatgg gcttttcgcc gggaaccgtc aggtcgagaa
    32581 agtaggcggt ttcctcgagg gtgtcggtgc cgtgggagac aatcactccg accacttcag
    32641 gccttgccat ggcggcgacc acggctttct gcagcccgat ccaccgctcg gggtccatgt
    32701 aatccgaagg gacgttggag agattttgaa cctcgatgcg ggcaaactcc ccgatctcgg
    32761 ggaccgtgga caaaaggtcc tccccggaga tggcgggtac cggggccttt tttaccgggt
    32821 cgatcttcat ggcgatggta cctcccgtgg cgatgagggt caccaccggg aggtccgcgc
    32881 gggcgagaaa gggaaacagg agcaggctca agaccggcat gaccacacag atgaatctcc
    32941 aggctttccg tgacgcaggt ttcatgacgt ctcctttctt gctggggttt tcgatcaacc
    33001 gcttctcccg ctgccaaaag ctcgttcggg acccggagcc tcggctactc gagactcggg
    33061 gttcggggtt ttcggcagcg gatgcgggct cgtccctgca gccgatagaa ccgggctggc
    33121 ggttgcaggg gtcaatggcg cggcggagcc gatcgattcg agcgggaggt tcaacgggag
    33181 ggtcaaccga gatcaccggg gaacgaaggc ctatagacta cacccgatgc gtgtccggga
    33241 aaaggacttc ctgggatgga cggcaaaagg ctttgaaaca tggccgacgc tgcacgccgc
    33301 cggtcaagga atgatgaaag agacgtaggt atatcccgag atatgaacat ggcggatctg
    33361 ccgaaccatg ttgcggatca ggtagagccc cagtgccttg caatcttctt cgctcgccca
    33421 ggcaaaggtt tccggccgcg cggggccgtc cacgtcgctc atgcgggtgg cagcgcggat
    33481 ttccacgaaa accccctcct cgagtcgcac gatcctgaac ttcacgcgtc ttttcggaac
    33541 cccctcgctt tccgattcgc tctggatgtg ctggaatact tcttcgcagg acagttgcag
    33601 gatggacgcc tgggaggggg tgagctgcag ggattccacc ccgtcggcca actgccggac
    33661 aaggtccggc agcttttccg ggaccccttc caccttgaag gaaacgcggg gtttgggctg
    33721 gaaatggtaa atggtgttga ggacaagggc catgagcccc ccggccgcca cggcccgggt
    33781 ggtgaaaggc tggagggcgg cgggcatggt tccggagaag aattcccggc tctcggccat
    33841 catcccgacg cacatgctga cgccgatgag cagccccatc tgggggctca tgcccgactg
    33901 gtggatcagg tgcaatcccg agtgaaacat cagggccaag agccccacca ggaacccccc
    33961 gaaaaccggc cccggcatgt ccaggatgag ggcgctgatc ttggggcaaa acgccagaaa
    34021 gaaaaggaat cccaccgcga ggaagcccac ggaacgggaa gccaccccgg tcattcccag
    34081 aacggggacg ttttctccgt aggtttcgtt gggcaaggtc ccggcaaggc cagcaagaat
    34141 gttggcggtc ccgtccgcat acaggcatcc ttggacccgg tcgtagtcca ccttcttgaa
    34201 atcgcggacg gaatagctct gggcgagcat gcaattgccg atggcctgga tggaattgac
    34261 cacggtgacc accacaaagg ccgccagcaa gggaaggtgt tcccatcgaa gatccagggt
    34321 cagccccggc cagttccccc gaggcaaccc catccactcc gagcctttga agtgttcgaa
    34381 gtccaggatc ccgaaggtcc acgccgccac ataccccact cccagggcca ggacgggacc
    34441 ccataccttg agtttcttcc ccgcataaag gttgatgctg agcatggtga aaagggtgat
    34501 catccccacg gccaggcttt cccgggaagc atagtggggt tcccccggat gccccatcca
    34561 ttccttgagg cagacgggca tgaggctgat gacgatgagc agaatcacga tcccccccac
    34621 agggggagtg atgatggttc gcaggtgcct gagaaagtag gataccagga gttccgccgg
    34681 ggcgctgagg acgctcaggg tggccaggag ggcaaaaccg cccgcatgga gcgcatccag
    34741 gctgcaggca aggtaggcgg tcgagctccc catgaaaatg acgaagcccg acccgaattt
    34801 gccaaagcga atcagctgca gaagggaact cacggcgctg atcaccaccg ccccgaaggt
    34861 ggcaaaggca atgtgctcct ccggagcatt ggccattctt cccaggatcc ccgggaaaag
    34921 ggtgttttct ccgtacatga tcaccacgtg ctggaaagcc agaaggatca ccatccacaa
    34981 agggggggat tcgtctaccg catacaggat cccgccgttt ttggacgtct cttccatgga
    35041 gtcaactctc gattcggcgc ctacgggcgc atggagtaca agctcaccgg ccgcacaccg
    35101 gggcggccca acggtggcac gagaaaggaa cacgaccccg gaaggaccgg aagatcctcc
    35161 caaaggtccc acaggcctcg tgcacggcac ttctaccaca gcacccggtg atctgccagg
    35221 aattatcccc caaagagctt tcacgggaga tttcaattca actagactgt gcaggagggt
    35281 ttgaaggttt tcgaggcatt tcggcaagga ggtggaagag tgaagaaggc acgaagcaaa
    35341 cgtttcctgg tttttgtgac ttttttactg gccgtcacat ttgccgccat ggccgcgctt
    35401 tgctcggccg acgaccacaa gagaaaaagg gaccattctc gaaaacacga tcccacgagc
    35461 tggagtgagg gctttggaag gttttccgga aaacatcacg acgagggcaa cgagaccacg
    35521 ggccaggtgg ctgcctggag ctttgccgcg gccaacctgg gcgtgtccct gagcctgctc
    35581 atcaaaggag tgaaccgatt ttcccccttg agcccccaat ggaagaacgc tctcaagaaa
    35641 ttcaacggca cccggaagaa atactcgatg aaaccgcact acctcctgaa cccgatcatc
    35701 ctggccttgg ctctgctcca ctattccctg tcccggtgtc cttcgacgtc gctgcccgag
    35761 tggggtctcc tggggatcgc cctcatgatg accctgggaa tcgtcatgaa gctgcggctc
    35821 tgtccaaact cccttctcaa gagcgtctac aaagtgcaca cccagccggt tgccgttctc
    35881 ttcgtgatct cgttgctggt aatcggccac atgatcgcgg actgagccgc ttgattcccg
    35941 cccgcctttg ccggcgggga taggctctcg cacgggacgt tcccctcacg gcgattccgt
    36001 gcgcccaacc ccccggggtc ggcgacgcgg cggccccgaa gacggggttt atccgccgtg
    36061 gtccgtctcc ggggccgtgg aagtcgagaa aacggcctcc taaaggacct ctcggatagc
    36121 cgctatgagc ttcgcttcgt cggggatgaa gaagcgctcg aggggcgggc tgacggggac
    36181 gggaatgtcc ggggccgtca ctcttcgcaa gggggccttg aggctcgaaa aggcctcttc
    36241 ggcgaccagg gcgcaaatct ccccggcaaa gcccccggtg cgggtggctt cgtggagcac
    36301 caccaggcgg ccggtcttgc gaacggaagc gaggagggtc tccttgtcca gggggaccag
    36361 ggtccgcaca tccaccactt ccacctcgat gccttcccgg gccagttgcg ccgaggcctt
    36421 gagggccacc cccagcatct tggaccaggt caccacggtc acgtcccggc cgggccgctt
    36481 cacatccgcc ttgccgatgg gagtgaggta ctcctcttcg gggacgggtc ccggaacgaa
    36541 atagagcaac atgtcctcga tgaatatgac ggggttttcg tcccgaaccg ccgacttgag
    36601 aagacctttg gcgtcggccg gggtcgatgg catgaccact ttcagaccgg gactgtgggc
    36661 cacccaggcc tcgaggttgt gtgcatgctg acatccggcc ccgaagccgg ctccgctctt
    36721 catgcgcacc accaggggaa acgtcgattt tcccccggac aggtagcgta gcttggccgc
    36781 atgattgacg atcatgtccg aggcgagggt gaaaaacgga ttgaacatga tctccaccac
    36841 gggcctgagc cccgccacgg cggcccccac cgcaaggccc gcaatggcgg cctcggaaac
    36901 cggcgtatcc ttcactcgac cggggccgaa ttccttgagc aatcccgccg taggcagcat
    36961 cggactgtcg tggatgctca ccccgactcc ttccccggcg acaaacaccc gctcgtcccg
    37021 gatcatctct tctcgaaggg cttgatggac ggcctgtccc attcccaatt gctgcatggt
    37081 atcttttcct ttccagggca tcgtgaaccg ttggaacatc ctcaagagac caggacatcc
    37141 tcgagggcgt ccaggggatc cgggtaggga ctctcctcgg cgaaacgcac ggcttccgcc
    37201 acgatttccc ggacccggga ttccaggtct tccagttcct gccgagtcat ccaaccccgg
    37261 tcgaggacct cgtcccgaag tcggggaatg ggacattttg cctgccatgc cgcaatctcg
    37321 tccttgggct gataatgctg atggtcgctt tctccgtggc cgcggcagcg gtaggtcttg
    37381 cactcgatga gagtcggtcc ctcccccgaa acagcccgct cccgggccct ccgggtagcc
    37441 tcgtagacgg ccggggcatc gtttccgtcc gccgtctctc cgggcatggc ataggcggaa
    37501 gccctttccg caatgttgtt tacccgggtg tgttcctcat agcgttgggc cccggcgtag
    37561 aggttgtttt cgcacacgaa gatcacggga aggctccaaa ggctcgccag gttcaaggcc
    37621 tcgtgaaagg acccctcgtc ggcggcgccg tccccgaaga tgcacgccgt cacggtcttc
    37681 tccttgcgat actgctgaga gaaggccgtt ccgacggcga tgggcagccc tcctcccacc
    37741 acggtggtgg tgcacagggc gttgacttcg ggcaccgcca ggtgcagggt tccgctcttt
    37801 cccttgttgc agcccgttct cttcccgtaa atttccgcca tgagggcctt ggggtccgct
    37861 cccttggcca ggagatggct gtgactccgg tggttcgaga tgagaatgtc ctcgggaagg
    37921 agtgcggcgc acactcccga tcccaccgct tcctgccccg tgctcaggat catcatgcct
    37981 gggattttgc cctcgatctt acacaactcc gtcagcgttt cctcgaaacg ccgggtgagc
    38041 agcatggacc ggagcatttc aagcttcttc tccttggtga tctccatcca ttctccttgt
    38101 ttcagtccgt ccttgattga gcgctgcgcg atccgctgtc ggcatgcggc agtgtccggc
    38161 cgggaagggt cgggccgccc tcggaggttt aggggacccg caacctcgga acgaatcccg
    38221 gcgcacttta ccagaaaacg ggccccgacg aaacggcaac gggaagcacg catttccgcc
    38281 gggcaggtcc tgggggacgg gaacgacgga atagggaggg acccgctcga gggatcagag
    38341 ctttcccgag cgcaggatcg agaggatgag ccatagaccc aggacggtgg caatgatgta
    38401 gcccgccagt ccgaataggg aggtcaggga aagggtctgg ggaccgaaaa agttcagggc
    38461 aaattccatc accttctggt cggaattcaa caccagggct gcagccagga tggaggccgc
    38521 aatgatcatc cccacggtca gccggttcac ccctttttcc agctgggcgt ccagtcgatc
    38581 gaacccgtcg tgcctcagtt ccacccgctg tttgccccgg gaggcggcct tgagaatgtc
    38641 gtgcacgtgc ttgggaaagg ctttcatgta cagccctgcg gtgcgggcgt ctttggccag
    38701 gtccttccgc atgctccggg cttcgacccc ctgcttcagg agctttttgg cataaggtcg
    38761 ggtcacctcg agaatgctgg cgtcgcttcc caggatcttt cccaaagcct cggtctgaat
    38821 gaaggtcttc aacaggagca ggatatttcg gggaagccga atgttgtact tgagcaccag
    38881 gtgcatgagc tgatcgtaca cgtcccttac ggagatgttt tgcagggagc gcccgtagaa
    38941 ggcttcgctc acgtccttga gatcgatgcg aaacgcgcgc aagtccatga ggtcttcgtt
    39001 gaggattccg gcatcgagaa gggcttccat caccaggtcg tagtcgtgtt gggcgaagcc
    39061 caggaagaga tgggcaatct ggcgcatggt ttcctcgtcc aggtacccgg tgatgccgaa
    39121 atccaccaaa ctcacccgac cgtcgtacaa gacgatggta ttgcccggat gagggtcggc
    39181 atgaaagaac ccgaactcca tcagctggcg ggaaaaggtc cgcaagccga tgagggcgat
    39241 ctccttgggg tcgattccgt gggcgcggat ggcatccacc tggtccatct tgattccgtc
    39301 cacatgctcc atgaccagga cggaccgggt ggtgtagtcc caatagactc tgggaatgtg
    39361 gatttcatcg acttcccgga aattatacgc gaacttctcc atgctccccg cttcgatgag
    39421 catgtcgagc tctcgaaaga tgactcgttc gaactcctgc accaggttca cgactccgat
    39481 catgtgcccg atctcgaagg acttttcgat gcgatgggcg atgaaataca tgaggcggat
    39541 gtcgtcccgg attttcgatt cgatccccgg tcgaatgatc ttcacggcaa ccttttcccc
    39601 ggaaaacagc agggccgtgt gaacctgtcc caccgaggcc gcggcgatgg aatccgggtc
    39661 aaatcgggca tagatttgat cgaggggtcg ccggagttcc ttttcgatga cggccttcac
    39721 atccttgaag gggatagggg gaacccggtc ctggagtttt tcgaattcgg cgatgtattc
    39781 cggggggaag atatcggccc gagtgctcat gagttgaccc agcttcacga aactgggccc
    39841 caattcctcg aggaccaggc ggattctccg gggggacgga aacccgcgga cgagcaggcc
    39901 tccctcctcc gggtcttttt cgggtctccc ggccttggct ccccacagcc gctcagccac
    39961 gtcccgaaaa ccgtgtttca ggaggacccg ggtgatggtc cccagtctct tgatttcctt
    40021 cactccccgc cagcttttca agagtcccat tcctcctccc ctcctgtcca cggcggcacg
    40081 gggaggaacc cgtctccgaa ggcgattccc gtccgtgggg cccctgttgc cgtcgccatg
    40141 gatttcattc cttcttccgt ctcggcatcc gtccgcggga tttgcgaccg acacgggttg
    40201 tttttcggga agaaccaggg ttcttccacc aatgggttga aactactcct tggccttgtt
    40261 gacgcaaaag agggcgaaga cgaagagtac gacgcccatg gaggcgagaa gaacataggg
    40321 aaaggcttcc ggtgctcctt cgaatgcggc ggtgcgtacg gcatgggcgg catgagtgag
    40381 aggaaggagg cccaggggct tctgaaccca ggacggcatt tggtccacgg ggaagaaggt
    40441 cccccccagg aaagccattg gcgtgatgat gaaattggtg agcagagcct ggtccgcatg
    40501 ggacttgacg agcatggcca tgcccaccgc cagggaggcg aaaacaaagc tgttgagaac
    40561 gacccccagc cagaacgcgg gactgtaaga aagcaccacc ccaaaactta gggcgatggc
    40621 caggatcacc agtaccgaaa ggagggcccg ggtaatcccc gccaggacct ctcccgtaac
    40681 ataggccatg cttccgatgg gtgccgcctg gaattcttcg aagatccgcc agtaaaagcg
    40741 ggcgatgttg atctcgctgg ctatggcgaa ggcctgggtc atgctgctca tggccaccag
    40801 gccggggatg agaaattcca ggtaacttcg cccctggatc tccacgtgcc gtcccatgcc
    40861 caggccgaag gccaggacgt aaagaagggg agccacggcc atggaagcga cttgcttgac
    40921 caacttcttt cgcaggatca agacctcgcg atggtagacg gccaggcatc ctttcatggc
    40981 aggaccttct tgcccgtcag ggaaaggaac acatcctcga ggttcaccct ccggagggag
    41041 aagcttccct gctgtcgggc gatatacgcg ttggcctccc ctcgactctg aaaataccgg
    41101 gtttccatgt tgtcatcgac catttcgtcc agggcccagg tcccaagccg ggccatcaag
    41161 gcctgggggg aatccacggc aacgatccgt ccttcgtcca gaaaggccac ccgttcggcc
    41221 aggaactcgg cctcctcgat gtaatgagtg gtgagaaaga tggtggctcc gctctgttga
    41281 atcctcttga tcaaggacca gatctttctg cggatggtcg ggtccagccc caccgtgggt
    41341 tcgtccagga acaggatgcg cggggagtgc atgagggccc gggcgatcat gacccgccgt
    41401 ctcattccgc ccgaaaggtg ctttaccggg ctgtccctgc gatccttcat gccgatgtag
    41461 tccagaagtt cctcgatcct cgacttgcgc tcgctgcgcg gcatctggaa aagcctgccg
    41521 tggatctcga ggttttcccg gacggtaagc tcctggtcca ggttgatggc ttggggcacc
    41581 agtccgcact gccgttttgc cttgagggtg tctcgaagaa tgtgaaaccc gtgaagggtt
    41641 gcgttgccgg aagtcggggc ggcaagcccc gtgaggaccc ggatggtggt ggttttcccg
    41701 gacccgttgg gccccaggta cgcaaaaagt tccccttccg ccacgtgcag gctgattccc
    41761 ttgagcgcct gaacgctctt atagttcttg gtgaggtttt caatgcgtat catttccggg
    41821 gcaatccaac cggttccgtt cctggggttc ctgccccact gcgcggggtg gaggtcttcg
    41881 cactttctct tcaacttccg cttcctgtca agttcataat cgccgccctt cgatcaacga
    41941 ttgaacaatg aattcaaaaa acttatatta cgaaaacttt gggaccggcc gtcccaggaa
    42001 cgcctctatc ccgagcggga cggcaacctg aagggttcga gacccccccg gcatccgcgt
    42061 aagcatcgag ggccgccggg gccggacggg ccggacggag ttccgccggt ctctgcagaa
    42121 cggaccgggt acatatctgc atgagtcctg aatcgcacgt ttctattttg tggaattcgc
    42181 ccgtcggatg tcggtgcgtt tcccggggcc gagccgggta cgtgggctgg acgaggggca
    42241 gcccacggca ttgcgccgat gaggaagctg cgtttttctg cccggggccc ggaactccgg
    42301 ccttgggcgc gtgcttttgc agtgggattc gcatggtaag ggaaataccg tatggcttat
    42361 gatttagggg tcgacgtcgg atcggtaagc atcaacagcg tcctggtgga cgaggatcaa
    42421 agggtggtct acgaggcgcc ctaccggcgg catttcgggt tggtctttga agaaaccctc
    42481 gcattcctcc gcagaattct ggacccttcc tttttcgccg ccgaaggaat cccttctcag
    42541 cccattcgct cggtttcctt cacgggggtc cacgggcaac tcctggcgga cgtcctttcg
    42601 gctccctatg aagtggaaac catcgcccag gccctggggg tgatgcacct ggtccccggg
    42661 gtacgctcca tcatcagcat gggcggccaa gacgccgcgt tgcttcagct cacctacgtg
    42721 ggagaccgct ggcacctgga agccttcaac atgaacggtc cctgtgcctc gggaacgggc
    42781 tccttcatcg accagcaggc ggagcggctg gcccaatccc tgtacggccc ggaatttcac
    42841 atggaccagg agaaacttca aaagaccctg gacgatttca tcgagctggg actcaagagc
    42901 acctatccgg cgcccgtggc cacccggtgc accgttttca ccaagtccga catgatccat
    42961 ctccaaaaca agggagaacc cctgcccaac atcatcgcgg ggctccacta cggaaacgcc
    43021 gccaactacc tgagtaccat cgtggccaac cgggagctcg aagaccccgt ggtgttcgtc
    43081 gggggcatgg cgtccaaccg cctccaggtc atggcctttc aacactattt ccccggactc
    43141 cgggttccgg cgcaccatac ttccctggga gccctggggc ttgcccttca aacccagcga
    43201 caggggacga gggtgcagat cgatctttcc cggttgaagg agtaccaatc cgcgttgcag
    43261 gaggaatttc ccgtggcgcc gaggctggag ctcgagtgga ctcgattcga ccccgacaac
    43321 agcctctctg cccggcacaa acgccggaag aaaaaccccc gggtctacct gggcgtcgat
    43381 atcggctcca ccaccaccaa gtatgccctc atcgacggca agggggaaat cctccacaag
    43441 tgttacgtcc cgacccaggg caaacccatc gaagtgaccc agcggctgtt gcagaacctg
    43501 gtccgggaga cgggaaaaca ggtgcagctc gccgccatcg ccaccaccgg ttcgggtcgc
    43561 aacgtggtgg gagactttct caaggcggac ctcatcatcg atgagatcac cgcccatgcc
    43621 cggggggccg tggaagtgga cccgctcatc gacaccatct tcgagatcgg gggtcaggat
    43681 tccaagtaca tccgcatcga gaacacccat cccctggact tcgacatgaa caaggtctgc
    43741 gccgcaggca cgggaagctt ccttcacgaa ctggccaaca agatgaagat caatatcgtg
    43801 ggggaattcc aggaaatcgc cctatcggcc cggaccccca ttcacctggc cgagcggtgc
    43861 acggtgttca tggagtccga cctgggaagc tacgcacaaa agggcgcccg gcgcgaagac
    43921 ctcattgcgg gactctgcta cgccatcgtc cacaactacc tcaatcgagt ggtggaaaag
    43981 cgccccatcg gtcagcgcgt catgttcctg ggaggccctt ccttgaaccg ggggatcgtg
    44041 gccgccttcg agcgggtttt gcagcgcccc atcctggttc ccaagcaccg ggaagtgctg
    44101 ggcgcttttg gggcggccct ggccgtgagg gagatgttcg agaaacagga actgtcccgg
    44161 cagagtcgag acctggaggc cctggcaagg atggaggtga gctacaccga gtccatttgc
    44221 cgggcggaca agaaatgcca caatgagtgc aagttgaagg tgtacgattt cggcggacaa
    44281 aagagtgttt gggggggaga ctgcggccgg tacgaggtga cccgctccac gggctgggtg
    44341 cggacgaatc ttttcctcga gtaccagcaa ttctttcagg aaatgatgga cgaggagggg
    44401 atctccgccc acgaggtttc cccgggggac cgcgccacca aaccccggat cgggatcccc
    44461 atggctctcc atgccgtcga atggggggtc ttctgggcgg tgctcttctc ccaactgggt
    44521 cattcggtgg tgttcagccc tcgaaccaac aaccgattgg ccctgagcgg cgtcgagtgc
    44581 atgaccgcgg aaacctgttt tccagtcaag gtgttccacg gccacgtgcg ccatctcatg
    44641 gaacaggccg actacctgtt tctgcccaac gtcatcaatg cgcccactcc ccaggaggat
    44701 gaaatcgggt tcttctgccc cctggtggag agttctcagt acatgtcccg cgcggccctc
    44761 aaggtccccg accccaggat catccggccg actctgcacc tcaaggacgg gccggaacac
    44821 ctgtcccacg ccttccacaa gggcttcccc ccggaactgc gcccaagtct gcgggagttg
    44881 gaaaccgccg tccaacgcgc ctgggatgcc cagcacgcct ttcgcgcacg gctcctgcgc
    44941 cggggacggg aagtcctgga acaactgccg cccggggagc cgctgtggat cgtgacgggg
    45001 cgaccttaca atctctacga cgaacggttg aacctccaac tgggcaggaa cctggcaaaa
    45061 agaaacatta ccgccgtccc catggatttt ctcgatctga atgccgaaga cctcagcgac
    45121 tttccgcaga tgtactgggg gctgggggcc agggtccttc gggccgcaaa agtcatcgcg
    45181 cggacccccc attggtacgg agtgcacctc accaatttca gctgcggggc cgattcattc
    45241 atcgaacact tttatcggca tatcctgggt gaaaagcccg ccctggtttt ggaactggat
    45301 gaacacagcg ccgtggcggg gctcctcacc cggatcgaag cctaccagaa cgtggtcaag
    45361 aacctcgagg aacaatcgtc gatctgcgac ccgaatccgc aatcccggca ggcgggtttg
    45421 gaggcgacag gctgatgacc cagaaatcac cggagtccca ttgcatggct aaccctcaga
    45481 cagtcgcatc ctttgaacga ctgaagacca gcgtggccca gttcgacgtt tccggcagga
    45541 ccctgctgat ccccaacatg gcacccacgg gagttcgact cctggcggcg tgctttcgag
    45601 ccgtgggggt gaacgcggtg gtgatggaaa cctacaaggg gctgaacctg ggcaaggaat
    45661 tcacctcggg caaggaatgc tttccctgcc aggtcaccct gggagacatc ctgtatcact
    45721 tgaaaaagga agaagagaga ctcggggagg acttttcacc cgggaactac gtttattttc
    45781 ttcccgaagc ggacggtccg tgtcgttttg gaatgtacaa caagatgcag cggctggtcc
    45841 tggatcgatt tccgacctac aaggacctgc ccatcgtcta cctgtccacc ggaaattcct
    45901 acgccacgtc ggacatcctc tctccccggt acgctcccat tttccgaagg ctcagctacg
    45961 tagccctcat cattgccgat gtgttcgacc gcaccgtctg gcgggtgaga ccctacgaga
    46021 ggcgcccggg gatgaccgac gagttcatgg acggagccat cgaagaaatg gcggccctca
    46081 tcgagcgcat cgggacaacc ctggacttcc cccaactgta ccggtgcctt gaaacgaccg
    46141 tggcgacggc tgccgggttc atcgaccctc agaaaccccg acgtccccgc atcggcatcg
    46201 tgggagagat ctacctgcgg acccatccgg actccaacca ggaaatcatc cggcaaatcg
    46261 agaaattcgg cggagaggtg gtggacgcat ccctgggaga gtgggtgaac tacgtgacct
    46321 acgagcagct tcagaagacg gcgagaaagt ggcgactggc ctggagggaa agggaccacg
    46381 gagtgttgcg caagctgacc ctgcagtgga tttccctgca gatcgagcgg tggtaccagg
    46441 cctggaggca gggccaggtg tatcggatcg ccatgcggca cttggacatc cagcaggatc
    46501 acgacatcgc cgccgtcgag aaacgcctgg accgggatcg cctctacagc ttcgccatcg
    46561 gcacggaagc ggccctgagc atcgggggcg cactggaata cgtccatgaa ggcttcgacg
    46621 gggtggtgaa cgtttttccc ttcacctgca tgcccagcac catctgcgcc tccatcctga
    46681 agcctctcct cctggacaaa cgcatccctt acctggacgc cccctacgac ggaacctacc
    46741 agcccaatcg ggaaatcgtg cttcggacct tcctctacca ggccaggcag cacatggaat
    46801 cccgccgggc caaacgagag aacggtaaga actgacatgt ccaatcaaac catgatcatc
    46861 aacgcagcct tccccgaaga atgccgcgtc gccattcttg aaggtcaatc cctggagggt
    46921 ctttttctcg acacggccag tcgcaccaag atcgtgggaa acctctacaa aggcgtggta
    46981 acccatatcc agaccagcct gcaggcggcc ttcgtcaact acggcatgga gcgcaacggg
    47041 tttttgcccc tgtcggagat ccaccccgag tactaccagc aggagccctc ggaacccctg
    47101 cggatccagg atgtcctccg ggtgggacag gaggtgctca tccaggtggt gaaggaggaa
    47161 gtgggcacca aaggcgccct gctcaccacc tacgtttccc tggcgggccg ggacgtggtg
    47221 ctcatgcccg ggcagcccca gcggggagtc tccaggaaga tcgaggacga gacccaccgg
    47281 gaacgtctca agcaactggc ccacgacttg cggattcccg aggaattcgg catcatcatc
    47341 cgcaccgccg ccgaggaccg caccaagagg gaggtcgcca aggacctgaa caacctcatg
    47401 aaaatgtggg aggagatcaa gaagcgggtc caggaaaccc cctctccggg cctcatccac
    47461 caggaacagg acctggccct gcgcgtcatt cgagactact tcaatcccaa catcaagacc
    47521 gttctagtgg acgatcggga aacccaccag cgggtcaagg acttcctgcg gatcatcgat
    47581 ccccggctcc agcgcatcgt caagtaccac cgagaggacg ctcccatcag cctcaagtac
    47641 aacctcgaag accagatcga acaggtcttc cagaagcggg tccccctgaa atccggcggc
    47701 tacctgctca tcgactccac cgaagccctg gtggccgtgg acgtcaattc cgggcgggcc
    47761 aacggggacg gagccgtgga ggaaatggtt ttcaaggtca acatggaggc cgccacggaa
    47821 atcccccggc agttgcgcct gcgggacctg ggcgggctcg tggtcattga cttcatcgac
    47881 atgcacgatc gccgtcacac ccgggaagtg gaacgtcgac tcaaagagga aatgaaaaag
    47941 gacaaggcca aaatcaccat cgggcggatt tcccgcttcg gccttctcga gctttcgagg
    48001 cagcacctgg gaatcaacac cctgcggagc tcctcgaggg agtgccccac ctgcaagggt
    48061 gcgggctggg tccgcactcc cctggccagc gccctcttcc atcttcgaaa aatctggatt
    48121 gggctggccc agcgaaaaga cctggcccag gtgagaggga ccttctccat cgaggtggcc
    48181 aactatct
//
LOCUS       DAOOVC010000014        47151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_486_length_47151_cov_6.622282, whole genome shotgun sequence.
ACCESSION   DAOOVC010000014 DAOOVC010000000
VERSION     DAOOVC010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 47151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47151
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_486_length_47151_cov_6.622282"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLM79_03430"
     CDS             <1..662
                     /locus_tag="PLM79_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV85386.1"
                     /translation="RRLAQLPPPIRMCDALSGNLPGTVKTILANCLTHGRRNFVDTIP
                     AFPEQAEYVIDILARVYHIDDPAKANRLSADQRLAFHQQHSAPLMQDLKEWLENQPAE
                     NSVEPYSSMGKAILYMLNHWPALTLFLRVPNAPPDNNFCERVLKQCILHRKNSLFFKS
                     LRRAWVGDLFMSLIATCRFSGVNPFAYLTALLQNTKKILQSPGEWMPWNYLHTLAGLP
                     P"
     gene            877..1428
                     /locus_tag="PLM79_03435"
     CDS             877..1428
                     /locus_tag="PLM79_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85387.1"
                     /translation="MRRRGVFLFNIASASDADIGKAVFVKDDQTVGLVAEVSNPIYVG
                     NIFEVESATKVWVEIYPALLQTDVATHISDASGAHAASAISVADSGSKITATDVEAAL
                     AEVMTGIKTAQYTLFPQAITLEDGTAVGKFTSEDQPSVGWAQLSNKELALRGSVPPKS
                     LNPGSRRGRFFMDHESQRNAEPV"
     gene            1823..2479
                     /locus_tag="PLM79_03440"
     CDS             1823..2479
                     /locus_tag="PLM79_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOV85388.1"
                     /translation="MVSITVDGKTIEAEEGSSLLQACLDHGIYIPNLCFLKEMDKPTG
                     SCRLCIVELEGEEKPVASCGVKVKEGMVVRTDTPHLRQLQRVDFQLIMSAHHMECKNC
                     LSRKKCELQKIAKTLEVRLKPTKFEELGRDLAVLKHPCFDLLPARCILCRKCLFVCGQ
                     NNEKPLLRLVRNGYESVIEATCEGEDPDKLPCRYCLACVEACPVSAIVPVARELDRGV
                     "
     gene            complement(2573..3637)
                     /locus_tag="PLM79_03445"
     CDS             complement(2573..3637)
                     /locus_tag="PLM79_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOV85389.1"
                     /translation="MRDEDKTREQLLEELAGLRRRVLDLESLENEHQRLLQALRESEE
                     RFRFMAETTGDVLYRLKYHCMKFDYISPSVVKLIGYSPEEINALGFSSLIMSLEGLEG
                     NTLSTEELTRNRKEGKTGEYRADYLIKTKSGDLKWVGDHSFPWRDETGKSIGSVGILS
                     DITERKRVEAALREMNQELHRLVTLDGLTQVANRRRFDEFLGQEWRRMRREQGPLSLI
                     LCDIDHFKLYNDSYGHQVGDDCLRVVAQAIRKSVNRPTDLVARYGGEEFAVILSNTDS
                     KGAVRVAENIRREVQTLQIAHSSSSERRYVTISCGVSSMIPTETRPAETLIALSDEGL
                     YEAKKRGRNRVVLKTLEEPA"
     gene            complement(3747..4874)
                     /locus_tag="PLM79_03450"
     CDS             complement(3747..4874)
                     /locus_tag="PLM79_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOV85390.1"
                     /translation="MEVEQTPGPGFSGTVEGIALTELVQLLCLARLDQCVAVRVGEVS
                     GQIAVAAGQVVHAVLGEVQGEEAVYELLGCGHGSFETKPLEEQPVQSVSKGWEYLLVE
                     SVHRRRKAGAGTGPEHAPGRGGFEPEGFRGLLRGIQLVDLIQLICSSGVDCRVEVEAG
                     SKKGTLLIRSAQVCQALTEDLTGEEAFYRILSWRDGRFQMSAGPQEVEKVVRKPWEHL
                     LVEAMRLRDESPPGDASEGPQTPESLVQRVGKMKVNDKVRLAMMGDKECRSLLMRDPN
                     RLVQAAIIGNPRITDGEIAAFANSRNVDDEVLRKIASNREWVKSYAIRMALVTNPKTP
                     LPIAIKLVPTLTQQDLKQLAKSKTVPIGVAQAARRITFQKE"
     gene            complement(5096..7165)
                     /gene="fusA"
                     /locus_tag="PLM79_03455"
     CDS             complement(5096..7165)
                     /gene="fusA"
                     /locus_tag="PLM79_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOV85391.1"
                     /translation="MKEDVARVRTFATISHGGAGKTSLTEAMLFNAGVTTRLGKVDDG
                     TSTMDYEPEELKRKITISTAFNTLTWKKHQINIIDTPGDFNFVAETKTSMQGADGVLV
                     VVDAIDGVRVQTEKVWEFADEFGQPRMIFISKMDRERADFAKTVEDIRNNFGKQCVPL
                     GIPIGAAETFKGVVDVISNKAYIYGQGDSGKFDLQDVPADLAGEVAEYMEMLTEQVAE
                     SDDGLLEKYLEEGELSTEEIREGLRASVVSGKLVPVLCGSGPGNIGIQPLLDLLVETM
                     PSPLDRGPREGKSPSGAPVTREPDPGAPFSALVIKTISDPYAGRLSVLRIFSGTLTED
                     STVYNSTKQTKERFGQLLRLKGKSQDPIESAGPGEIVAVAKLKDTTTQDTLCTEKDAI
                     LFAPVELPPAVYSLSVEPKSKGDEEKIFSSLSRLMEEDLTLKLERNDETKEMILSGMG
                     EIHIEATVEKLKRKFGVEVNLKLPKVIYKETIKGKARVQGKYKKQSGGRGQYGDCWIE
                     MEPMPRGEGFVFEDKIVGGVIPRQYIPAVEKGIVEASGEGVLAKYPVVDFKVGLVDGS
                     FHPVDSSEMAFKIAGSMAFKKAMGDAKPTLLEPIMVMEITVPDDCMGDVIGDLNSRRG
                     RVLGMESKGKKQIIRANVPHAEVLKYAPDLRSMTAGRGMFTMKLSHYEEVPAALQEKI
                     IEAAKEE"
     gene            complement(7503..8498)
                     /locus_tag="PLM79_03460"
     CDS             complement(7503..8498)
                     /locus_tag="PLM79_03460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOV85392.1"
                     /translation="MIKLILIAACAFFTVVLVMPLVKRTALRWGFVDEPGPRKLHLRP
                     LPRLGGVAVYLGCMAALAVFDLFYLSQLVSILLGATFVSFLGIWDDYRVLGPVAKLSG
                     QVCAAVILLLSGVQVTFLPHPVLNVAVTIFWVVSITNALNLLDNMDGLSAGTATVACA
                     FFLLLAVMNKQFLVGSLAAALLGACVGFLLYNFNPAGIFMGDSGSLFIGFVLAAIGIK
                     LRFPDNVAVVTWMIPVVVLGVPIFDTTLVVVSRLRRGLNPIVSPGKDHLSHRLLQLGC
                     TQREAVLVLYLLCGALGVLSVVLMHATRTEGFVIGALLALAGLWALWRLEKVPVS"
     gene            complement(8495..11881)
                     /locus_tag="PLM79_03465"
     CDS             complement(8495..11881)
                     /locus_tag="PLM79_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV85393.1"
                     /translation="MPSRFVVYCDSAIEAGWLLSVMVVPLFFNPHSSRIFEPDKLLVF
                     RSIALVSAALWLAKGIEVLPGSVRSGRSTAGANASATPWEALKQAVRVPLVLPVVLFL
                     ATTLLSTSLSVLPAHSLWGSYERAQGFFTTLAYGTVFFSLLAALRQQEQLERLLQIVL
                     ITSIPVTLYGTAQHFGWDPLVWEKEIEGRVSGNMGNPIFLGAYLIMVVPVTLAEMLRR
                     YMKFRSSPGSRVWNVAALLLLLILLGLQVSCILFTQSRGPWLGLLCGISFFILLGLIA
                     LRPAEPGEGAARIKEVFKAFVFALASIPVGVLPAYVFFALTHRGRRWLWISFVFHGLL
                     AALFLLLLNMPASPLHSLRGYPYLGRLAQLSDVETGTGKVRMLVWQGTARLIAGNPGR
                     MWFGHGPETMKFVWDPYAPPELAHYEYRNAAPDRAHNETLDAVVTTGLAGLAAQLLVV
                     VLVFRYGIQWLGLMPGASHGRVFYGLVFLGMLGGTWVSLRLHGSFALLGLGIPAGMLL
                     GVVVSVLVAGFGVRPREETPLPRERQVVLIALLSGLITHFVEIQTGVSVAATRLYFWV
                     YCAVVVVLGTGRVVPEDSSPGVGGETKEPGARPPSAGKRGKRKKGKVPVRIEGESGAF
                     SRLVGRRTVMAGFAALGFALFTLAFDCTLNPFLREETLESVWRSVTTVRLGSGVRTVY
                     GMPAVFLGTWLLAGTLLWAIHRSESAQGREPRSRLSGVAAWGLSALFGVGGTGLFLLV
                     MRPGSEAQSIVFYSWAVLGAGTVLGGILAAGKPWEQWISNSWKWVLYVAVGAVACWGG
                     VVFNLRPVQADIHFRMARDLERQKKWEPAARHFGEALRLAPEQEHYHLMLGRFYFHRA
                     GASRDREKDALYSRVEKAMSRAHELNPLNTDHLANLALLYWKWAESVSSDAQRQGKLE
                     QAHHFYDLAVKNSPRRVVLYNNWARVYLYQGAIEKAHEKIEASLAVDDRFADTYLLQG
                     EVFHREQKGREASAAYRKAVSLDPENAELWATLGQIYIRQGDERASLEANLKAVEINP
                     GLVGPHSLLGLIYYRWGKFHEALEENYKVLELQPDNVATYRNLALIYERMGRPEDAAA
                     QLEKIRKLSPESEHGKIRDSIDKMKARTGARGLEEDRGSP"
     gene            complement(11952..13079)
                     /locus_tag="PLM79_03470"
     CDS             complement(11952..13079)
                     /locus_tag="PLM79_03470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85394.1"
                     /translation="MAHRGPGICVLHIYKDYPPVLGGIELHVRLLAEAQARMGYDVTV
                     LTASPGLLSEEETRNGVRLIRAGRLGTAASTPISLPMPRILRRLRPHLAHLHFPYPWG
                     EVSQLLFGQAPCMVVTYHSDIIRQKTLLRFYRPMMNRVLSSARGIIVTSGAYLESSPY
                     LGEFRDKCAVIPLGIDLKPFLRSYPSRVRLLREFFAAPTVLFVGKLRYYKGLETLIEA
                     VADLDARLVVLGEGPLDRKLRDKVLSMKIGDKVAFLGEVTQEMLPAYYQAADVLVLPS
                     SHRSEAFGLVLVEAMASGLPVISTELGTGTSFVNVHGKTGVVVPPGDPVSLKAALSVL
                     LEDGELRRQMGSRGRVRALTEFSATKMVHSVCRFYDMQLRA"
     gene            complement(13142..14143)
                     /locus_tag="PLM79_03475"
     CDS             complement(13142..14143)
                     /locus_tag="PLM79_03475"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV85395.1"
                     /translation="MKFLITGGAGFIGSHLAEYLLGRGHEVVVLDDLSTGSWENVSHL
                     EDHPGFQIVVGSILHEFLVDRWVERCHAVFHLAAAVGVELIVKNPWESLITNIKGSEL
                     VLNVAHRYRKKVLVTSSSEIYGKNSNAPLREGDDRILGSPLKSRWAYSTSKAVDEILS
                     YLYWKEKRLPTVIVRLFNTVGPRQTGAYGMVIPRFVCQALRGEAITIYGDGNQTRCFL
                     HVRDAVGALAGLMDHREAVGDVFNIGSQEEVTIDELARQVLQLTGSPSPVVRIPYDQA
                     YGEEFEDMPRRVPDITKARDLIGFHPSYDLRGILTDVIRHYEEGRLPEAGSRRRLGP"
     gene            complement(14140..15252)
                     /locus_tag="PLM79_03480"
     CDS             complement(14140..15252)
                     /locus_tag="PLM79_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOV85396.1"
                     /translation="MRVALDARTATEHFPGIGRYVAGLSSGIREADPRLPVRLLADPR
                     NTLSWRPPQWARIPAPWSPFSLSQHWKLKPALRRNGITLYHSTYYPAPSFRDIPTVWT
                     CCDLIPLVFPRFFPRWQRVVFRIAHRVLVERCRHILAISDSTRSDLLRFFPARPEKIS
                     VIPLGVDSRFVPRGRDEIQGMRRRVGLPEKYVLYVGINKPHKNLVTLLKAWRDFRREA
                     RSSGDDVRLVLAGPWDERYPQAKAYAAREGLQGEVLFVGPVEEDLLPSVYSGARAFVF
                     PSLYEGFGLPVLEAMACGTPVLCAHTSGLPEVCGDAALYFDPRQYRTLAEGLRRVLED
                     AALREDLRNRGLDRASAFSWARTARETIRIYRKHSI"
     gene            complement(15218..16366)
                     /locus_tag="PLM79_03485"
     CDS             complement(15218..16366)
                     /locus_tag="PLM79_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOV85397.1"
                     /translation="MRVGIDVTAVLRGPGGMARYARELTTALGDVDRETRIIPLSVGS
                     GCPTVVPRRPGSRPPNAAGAERAFRLQTLLAHMVGASQERRFPLLDLFHGTDNLLPAF
                     RRVGTVLTLHDFVFRLFPRTTAPLNRLYLKLAMPHFLRRADALIAVSNSTRMDAERLY
                     GVTPQRVHVVPLGVSERFAPRDPGAVRRSLGDRYRLPVDFLLHVGALEPRKNLQVLLE
                     ALRWMGDERPTLVLAGPPGRGVREIRDDPAAVYGDGRVISLGPVPEEDLPGLYSLARA
                     FVFPSLYEGFGLPVLEAMACGTPVLCSKAAALEEVVGDGALRVDAEDVAGWAEAVRAL
                     WSRSGLRQELRRRGFRRARGFTWAATAQKTLEVYKEVHARRSGCSNRH"
     gene            complement(16445..17374)
                     /locus_tag="PLM79_03490"
     CDS             complement(16445..17374)
                     /locus_tag="PLM79_03490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV85398.1"
                     /translation="MSQAGETRHRLIHADARDLSFLPDESVHLVVTSPPYWNLKRYNE
                     HPDQLGHIEDYEAFLSELEKVWRHLFRILVPGGRLVCVVGDVCVSRRRFGRHLVFPLH
                     SDICVSCRKIGFDNLNPIIWHKISNATFEVANGCKFLGKPYEPNAIIKNDIEFILMQR
                     KPGGYRRPTEKQRELSKIKKKDFDGWFRQIWHITGASTKDHPAPFPLELASRLVRMFS
                     FVGDTVLDPFCGTGTTLIAALRSGRNSVGIEIDDHYCRATARYLKAETANLNMFYKAH
                     LSFEKLDMDESGSLHVCENQALYNVRSAKRTMV"
     gene            complement(17371..18054)
                     /locus_tag="PLM79_03495"
     CDS             complement(17371..18054)
                     /locus_tag="PLM79_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HOV85399.1"
                     /translation="MAHYWATRETQRKKQELRGQSDAGLRSAVTGGAQMDGFIDLFTA
                     LITETGLPMKFIFRKTALELPGYFRPTKEWDLIVVRDGTLVAAIEAKSQVGPSFGNNF
                     NNRTEEAVGSALDLWTAFGEGAFNAGAQPFLGYFFMLEECPASHRPVKVKEPHFNVFP
                     EFVGASYAKRYELLCRKLVLERHYTSACFLTSRSDAGLRGEYHEPAKDLSFERFAKSI
                     LSHVGAFAQ"
     gene            complement(18409..19482)
                     /locus_tag="PLM79_03500"
     CDS             complement(18409..19482)
                     /locus_tag="PLM79_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOV85400.1"
                     /translation="MKRAKVGIDVQSTRGRKTGIGVYTENLLAGLRRVARQYECLPIS
                     RGRSGDFRTHRRLLWQQVLLPLQARIAGVRLLHIPGFDAPRWAPCPTVLTVHDLIGAL
                     FPGNFPPVSRWYWSSWLPKSIRWADRVIASSHSTRRDILRLTGVPGEKIHVVYPGVGE
                     EFRPVEDASRLERTAAKYRLPRKFLLFVGTLEPRKGVDVLLEAFRLLAGETDHHLVLV
                     GRAGWGVEDLPGQLRRADFCARVHWKGFIDPGDLANLYNLAGLLVFPSRYEGFGLPPL
                     EAMACGVPVVCTETSSLPEVVGDAAIRVPVGEAHSLAEGILRALTDRELRERLRTLGL
                     HRARTFSRDDAAGQVAAVYDSLL"
     gene            complement(19479..20600)
                     /locus_tag="PLM79_03505"
     CDS             complement(19479..20600)
                     /locus_tag="PLM79_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV85401.1"
                     /translation="MGVHPGRTGTLAVVSMDIRRDLIAPMHRFEQLRIRHFFRRAPYN
                     DLRSSDWDESLIPFKGPLDLLRLLRREKPDILQTVEILSLGQLPYTAAIFFYAVLRRI
                     PLVAGVHVARPLEEKYGRVPARLLRALLQPVVRRTRLFFHVNEGASKNLEWMGVPASK
                     TVRHMYGTWGVDPKEFSPERDGREPAWGEGTVVLFVGRVHRQKGIFDLLEAFQQVYRK
                     NSRAILAILGDGPHRAEARRRALSRLPERAVRFLGTIPHGDLPPYFRSAAVLASPSRT
                     TRGWEEYVGMTNLQAMACAIPVVSTRSGAIPEFVPHGEAGILVEEGDVPGLASALLDL
                     LEDEDLRRRMGAAGRARVLDRYDAGENVRRAERIILERC"
     gene            complement(20603..21589)
                     /locus_tag="PLM79_03510"
     CDS             complement(20603..21589)
                     /locus_tag="PLM79_03510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85402.1"
                     /translation="MKTPPVTVMLPTYNRAHFLPTAVESVLGQTRGDFELVVVDDGST
                     DETPAVMEAFRDERIRLIRQENRGISGALNRALAHARGEIIARIDSDDRWLPNLLEKE
                     MGILEKRPEVGLVYSRAQAMDETGRPLAQVLGVPERYPGETLKSLLYGDFVCTITTLV
                     RKRCLDEAGPFDESLSGNEDWDMWIRLAPVCKFHFLDEVLAHFRMHSHRTTGKGSPLF
                     DRVARGRFRTLDKAFGRKDLGAELLQVKGLAYRNAHMDVGLRWLSAGEISRMGHHFRC
                     AFREGGNPVATLARICFLVLHYRYLSKRTWGVRLVERLAAAGRRRRGRDRED"
     assembly_gap    21688..21697
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21698..23087)
                     /locus_tag="PLM79_03515"
     CDS             complement(<21698..23087)
                     /locus_tag="PLM79_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOV85403.1"
                     /translation="MPVRTEEESLGTRVARGGIWVALSSWFNVVFGFLANIVLTRLLS
                     PEDFGIFALGMFFFSLLNLRPRIGIGQAFAQRRETDGELIGTHLALDLTAGLCTLLIV
                     SLSLPVLGWFEYPVEVGWATLALGIIGISDSVLGTAWVMLDKELHFSKTSLVSSLAFP
                     LSYVPGLWLAWKGGGYWSIVVQNGAYALLLLGGMWWTARRKLPELWNLPWRWNGRTAR
                     ELVRFGGVVGLASIAGLLVNQLDNYLVGTFLGLATLGFYDRAWRIAMWPSLLVGNVIH
                     RAGFFTYARFQGDPERLRRVVMLTVWLVATLALPLALAVFVSARDLVELLYGPRWVPS
                     ALLLRFLVALSVLRPLQDNAGSFFMAVGRPGLPARMTAIQAGVLALSAVPLTLAYGAV
                     GTCLGVGISYAVSLPLLYRPLFRTLELSPADTVVLPALAACLALGVSWVFSDRVGLET
                     LSLAGKVLVKGAV"
     gene            23388..24305
                     /locus_tag="PLM79_03520"
     CDS             23388..24305
                     /locus_tag="PLM79_03520"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOV85404.1"
                     /translation="MELFRTYLRLERGVSPHTLRAYQSDLEQFHEHLLTKTGSPSVSV
                     DEITSDLVRSFLAVLHSKVEKTSQARKISALRSFFGFLHERGCTDKNPASGITHPKIK
                     SRIPSFLTVDDVFHFLDSLRQWARTPGASWRRSRNWALYECLYSTGMRVSEVVGLNEE
                     DLFEARGMVLVLGKGKKERIIPVGKKALEAVDQYLRTLEEQSPGLRTKSPALFVNARG
                     DRISTRSVHRILLAELKRCGLWKPLSPHGLRHSFATHLLNSGADLRAIQEMLGHSSLS
                     TTQRYTHVHLEQLMKTYDAAHPRSRRKSP"
     gene            24384..24923
                     /gene="hslV"
                     /locus_tag="PLM79_03525"
     CDS             24384..24923
                     /gene="hslV"
                     /locus_tag="PLM79_03525"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOV85405.1"
                     /translation="MADTLFRGTTVLAIRHNGQVVVAGDGQVTLGDTVVKHQAKKIRR
                     LYHNKILTGFAGSTADAFTLFERLEAKLEQFNGNLKRAAVELAKDWRMDRALRRLEAL
                     LIAADADQCYILSGTGDVIEPDDGLAAVGSGAPYALAAARALTANTQMSAREIAEESM
                     KIAASICIYTNREFTVEEL"
     gene            24928..26304
                     /gene="hslU"
                     /locus_tag="PLM79_03530"
     CDS             24928..26304
                     /gene="hslU"
                     /locus_tag="PLM79_03530"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOV85406.1"
                     /translation="MQQPLTPAEIVRELDKYIIGQKDAKRMVAIALRNRWRRQQVPEH
                     LRDEIAPKNIIMIGPTGVGKTEIARRLARLAQSPFLKIEASKFTEVGYVGRDVESMIR
                     DLMELAVSMVRTEEMEAVKLKAEEIAEEKLLDILLPPKRQPENREQLLIEPDARQAAQ
                     EESDQADSTREKLRKLLRAGSLDDRYVEIDVPDRNFPMVEIFAGSGMEDMDYNLREML
                     GSMLPRRTKRRKVKIPEAREILVQEESQRLIDMDKVIKSAVERVEHSGIIFLDEIDKI
                     AGRESGRGPDVSREGVQRDLLPIVEGSTVTTKYGMVRTDHILFIASGAFHISKPSDLI
                     PELQGRFPIRVELTSLTRDDFVRILKEPENALIVQYKALLTTEEVELIFEDDAIEEIA
                     EIAYQVNSRTENIGARRLHTIMEKLLSEISFNAPDLKGQTIHITRENVRQTLMEIIKD
                     EDLSRYIL"
     gene            26321..27229
                     /gene="argB"
                     /locus_tag="PLM79_03535"
     CDS             26321..27229
                     /gene="argB"
                     /locus_tag="PLM79_03535"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOV85407.1"
                     /translation="MEAFLKDQCTVIEKARILIEALPYIRRFSQKTLVIKYGGHAMVA
                     DDLKESFAQDIVLMKYIGMHPVVVHGGGPQIGQMLERMGKKSDFLAGMRVTDQDTMDI
                     VEMVLVGKINKEIVSLLHRHGGQAVGLSGKDGHLIEARKLHVYRYQGDDEPPEILDIG
                     LVGEVSRVNTQILRLFEHSRIIPVIAPVGVGENGETFNINADLVAGSVAGALQAKKLI
                     LMTDVPGVLDARGELISSMTVGEAADALQDETLRGGMIPKVRCAIDAVQSGAEKVHII
                     DGRVPHALLLEIFTDAGIGTEISKKR"
     gene            27271..28461
                     /locus_tag="PLM79_03540"
     CDS             27271..28461
                     /locus_tag="PLM79_03540"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HOV85408.1"
                     /translation="MNDTQTASRQHIFNTYARLPVTLVRGEGCRVWDDTGKEYLDFLA
                     GIAVCNLGHCHPAVTRALCEQAQRLVHVSNLFYTRPQVELAAELTRLSFADKVFFSNS
                     GAEANEAAIKLARKAAKERHGEGRFHIIAMKDSFHGRTMATLSATGQEKVHKGFEPLV
                     EGFTFVEFNSVPAVEAAVTEKTCAVLVEPVQGEGGVRFPRPGYLAELKELCLKHDLLL
                     IFDEVQVGMGRTGTLFAHEQEGVTPHIMTLAKALGNGLPIGAMLAVEEVAAAFVPGSH
                     ASTFGGTPLVTAAALEVVKVLSNPAFLGGVRETGAYFREKIEALRPRHPVIREVRGRG
                     LMVGVELTVPGQKVVELCLENGLIINCTHENVLRFVPPLIVERQDIDRCLSVLEEALG
                     KGTS"
     gene            28458..29378
                     /gene="argF"
                     /locus_tag="PLM79_03545"
     CDS             28458..29378
                     /gene="argF"
                     /locus_tag="PLM79_03545"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOV85409.1"
                     /translation="MKRDLLTLRDLSPEEILYLVSRARELKAALAQGVLAPTCEGKIL
                     GLIFVKPSTRTRVSFETAMYRLGGRCLFMTAQDTQISRQEPAADTARVLSRYLDGMVV
                     RTFAQEELETLAKFSTIPVINGLTDLFHPCQILSDLVTIQERRGSLEGLRVAWIGDGN
                     NVAHSWINAAALLGFTLFLACPPGYAPDPAVLEWARAQNRGEIHRLEDPKEAVKSADV
                     LYTDVWASMGREEEAAVRREAFRPYQINEELLGAAPSHAMVLHCLPAHRGEEITDAVM
                     EGPQSVVFDQAENRLHLQMALLEWLLNQVR"
     gene            29401..30612
                     /locus_tag="PLM79_03550"
     CDS             29401..30612
                     /locus_tag="PLM79_03550"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOV85410.1"
                     /translation="MKVQKVVLAYSGGLDTSIILKWLIETYQCEVVAFAADLGQGEEL
                     DGLEAKALATGAVKARIEDLREEFVRDFVFPAFRANAIYEGQYLLGTSIARPIIAKHQ
                     IRIAREEGADAVSHGATGKGNDQVRFELAYLALEPGIKVIAPWREWDLRSRTALIEFA
                     GKRGIPIPVTDEKPYSSDRNLLHISYEGGILEDPWLEPDPKMFTLTTDPQEAPDQPEI
                     IELQFERGDAVALNGTRLSPARMLHELNVLGGKHGIGRVDLVENRFVGMKSRGVYETP
                     GGTLLREGHRAVESITMDREVMQIRDSLVPQYSRLIYNGFWFSPEMLLLQDMMDRAQE
                     NVWGTARLKLYKGTCRVVGRKSDRSLYQPSFATFEEDDVYQQGDATGFIRLQALRLRI
                     ESLVRSGLPRH"
     gene            30649..32055
                     /gene="argH"
                     /locus_tag="PLM79_03555"
     CDS             30649..32055
                     /gene="argH"
                     /locus_tag="PLM79_03555"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOV85411.1"
                     /translation="MAEKLWQGRFDQPTNKRVEDYTASIHFDNRLYRYDIEGSVAHCR
                     MLGECKIISHDDASLIVQGLGEILREIERGELSLDAAMEDIHMAIEQRLMQKIGEAGG
                     KLHTARSRNDQICLDMRLYMRDVLLQCRSAILDVRKTLVSLAQQNLHVIMPGFTHLQH
                     AQPVLLSHHLMAYYEMLGRDDERFSECFRRTNVLPLGSAAMAGTSLPIDMTWTAKYLN
                     FPRVTANSIDAVSDRDYLIEFCGASSILMMHISRLAEELILWSTVEFGFIEISDAFCT
                     GSSIMPQKKNPDVPELMRGKTARVFGNLMALLSLTKSLPLAYNRDLQEDKEPVFDTVD
                     TVLSTLRLLAKLLPEIRFMDARMAEAAAAGYTLATDLADYLVRKGVPFRRAHHVVGQI
                     VQYCVQEKKELQDCSLETLKNFHKAFDADVFQSLDLLGAIDQRISRGGTATSRVQEAI
                     EQAKKDLEVEETGLKNPA"
     gene            32144..33232
                     /locus_tag="PLM79_03560"
     CDS             32144..33232
                     /locus_tag="PLM79_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85412.1"
                     /translation="MILPNPCDTRLHNVLWGIPVLLLGLLLAGCGTKSFPRSLCDDAP
                     SVVQDLQAQVRLKRVELQWSVPGVEPGNVKKSGHRFSVLKSEIRWENRNCLECPVPMQ
                     HEIQRIEPPYAGFLTPDSTKISFADGSVEINRAYRYQVALLDKRGAQVTLSNPVIVKV
                     MPAPGIPRELLAAPENQGILLQWKPPVKDEHGRPIQGEVQFAIERHAPDNPWERVTSI
                     PIRGNLFLDPAVTSSETYDYRVIPLVVFEGTSILGEPASILHVKAPGALPPPPPNTVW
                     VIPGKGSLEVHWTEHKTKVGGYHVYRREGKEIIRLTAGPIQHPPYVDKSVKKNNVYHY
                     AVSAVGLQADQREGLLSKWAEIRALMFE"
     gene            33264..34520
                     /gene="lysA"
                     /locus_tag="PLM79_03565"
     CDS             33264..34520
                     /gene="lysA"
                     /locus_tag="PLM79_03565"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOV85413.1"
                     /translation="MHHFQFRNGELYCEEVPLREIVQRVGTPCYVYSHATLHHHFSVF
                     DGAFSEVAHLTCFAVKANSNLAILRLFGTLGGGADIVSGGELFRALKAGIPARRIVYS
                     GVGKTAEEIDYALSQGILMFNIESMEELEAVNARAAARKCRAGIALRVNPDVDPQTHP
                     YISTGLSKNKFGVDIENAVKAYQKARTLEHVEICGLGCHIGSQLVRVEPFVDTMKRLR
                     ILLERLDGLGISIRYLDLGGGLGIPYDRESPPHPSEYAEAILEELRGLPCTLIFEPGR
                     VIVGNAGILLTRVLYTKETPTRGFLVVDAAMNDLIRPSLYGSYHQIQPLRKEDVQTRV
                     VDVVGPICESGDFLARDRELPVVSSGECLAVMSAGAYGFTMSSNYNSRPRAAEVLVKD
                     RDWFVIRRRETWEDLVRSESIPDFLQ"
     gene            34546..35415
                     /gene="dapF"
                     /locus_tag="PLM79_03570"
     CDS             34546..35415
                     /gene="dapF"
                     /locus_tag="PLM79_03570"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOV85414.1"
                     /translation="MPEESRKIRFYKMTGSGNDFILIDNRTGRFDPDRSRSLVRAACR
                     RKLSVGADGLIFIENDGEADFKWRFFNADGSEAEMCGNGARCAARFAHLTGITTRLNL
                     SFRTLAGIIQAEIKGDRVKVRMTSPHGLQTDLHLSANERPFLLHFINTGVPHVVHLLE
                     KESDLEETDVERWGRLFRFHPHFHPAGTNVNFARVEDAGHMAIRTYERGVEGETLACG
                     TGSIASALVAASKDLVQSPVEVRTRSGEVLTVYFRGPTGPGGPEGASFTDVYLEGSAK
                     VVYEGELWDETLQ"
     gene            35434..36309
                     /gene="dapA"
                     /locus_tag="PLM79_03575"
     CDS             35434..36309
                     /gene="dapA"
                     /locus_tag="PLM79_03575"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOV85415.1"
                     /translation="MFQGAMVAIVTPFKNGRVDEESLRQLIDFQLANGTHGIVPCGTT
                     GESATLSHEEHERVVEITVEQVAGKVPVIAGTGSNSTEEAVRLTKHAKKAGADAALMI
                     SPYYNKPTQEGLFLHFQKVAAAVDIPIVLYNIPGRTAVNMEADTIARLAKIDNIVGIK
                     EASGSLKQVTDIIARCPGDFTVVSGEDYLTYPMLCVGGRGVISVVSNVAPKDMADLCN
                     LHFSGKQAEARELYYRLLPLCHALFYETNPAPAKAALAMMGKIPSEEVRLPLAPMSEG
                     NRNRLRKDLEAYGLL"
     gene            36426..37229
                     /gene="dapB"
                     /locus_tag="PLM79_03580"
     CDS             36426..37229
                     /gene="dapB"
                     /locus_tag="PLM79_03580"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOV85416.1"
                     /translation="MVRAAVSGIAGRMGTRIAQLIGETDGIALTAGFEHESHPAVGED
                     LSRVLGGKPTGRIIAGSIEDVLEEVDVVIDFTMPAASLRHLEMAASRGKAMVIGTTGF
                     SREQVAQIRQPASKIPCVLAPNMSMGVNVLFKLVGDAAKLLGSGFDVEIIEAHHRFKK
                     DAPSGTAVKLAQVAADALGRDLEASGVYARHGIIGERTETEIGIQTIRAGDIVGEHTV
                     IFGGMGERIEITHKAHSRDNFARGAIRAALWVVNQPPGLYDMQHVLGIR"
     gene            37367..38533
                     /locus_tag="PLM79_03585"
     CDS             37367..38533
                     /locus_tag="PLM79_03585"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOV85417.1"
                     /translation="MKIIKAERLRRLPPYLFQEIDRLKAELVAKGVDVINLGVGDPDL
                     PTPDHIIRRLQTAAEDPETHQYPSYSGMNDFRVSVAGWYRRRFGVELDPLREVLTLIG
                     SKEGLAHLPLACINQGDLALVPSPAYPVYHVATLFAGGESFFMPLQRENRFLPDLKAI
                     PPDVARRARLMFLNYPNNPTGATAERDFYERVVEFAREYEILVCHDAAYTEMAFDGYR
                     PMSFLEIPGAREVGIEFHSLSKTYNMTGWRLGFAVGNAQILEGLGQVKSNIDSGAFNA
                     IQHAGIAALEGSQECVGRMRQIYQERRDVLIAGLKKVGLDPDPPKATFYVWCPTPKGY
                     NSKDFTSLLLREAGIVTTPGSGFGEPGEGYVRMALTVTKERIEEAVGRIRKLSF"
     gene            38591..39097
                     /gene="folK"
                     /locus_tag="PLM79_03590"
     CDS             38591..39097
                     /gene="folK"
                     /locus_tag="PLM79_03590"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOV85418.1"
                     /translation="MDPMEQVFIGIGSNRGDPAGICRDAVGALDRTGSVRVLRMSSLY
                     RSKPVGVLDQDWFVNGVASCETDLEPLELLDRVLEIEKAFGRRRLVRWGPRTLDLDLL
                     CYGRRRVDHWRLTIPHPRLHRRLFVLVPLAEIAPDWVHPVRQLTARQLIDQLMKQDHD
                     QVVVPLAG"
     gene            39100..39372
                     /locus_tag="PLM79_03595"
     CDS             39100..39372
                     /locus_tag="PLM79_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85419.1"
                     /translation="MARILLILLLLFIGYRIFKRFSSPGKQVPFGWGDRREGTGDAEL
                     VQDPQCGTYFLPGSGVSARIDGRKLTFCSEQCRDDYLKNRSPSKGP"
     gene            39391..40053
                     /gene="fsa"
                     /locus_tag="PLM79_03600"
     CDS             39391..40053
                     /gene="fsa"
                     /locus_tag="PLM79_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOV85420.1"
                     /translation="MKFFIDTANLDEIREAHSIGILDGVTTNPSLIAKEGIAGREAFE
                     EHIKTICELVQAPVSAEALSMVSGDMIPEARSLAKIHDHVVVKIPMTTEGLKAVKQLS
                     EEGIKTNVTLVFSPLQALMAAKAGATYVSPFVGRLDDIAHDGMELVEQIVEIFSNYLF
                     ETEIIVASVRNPLHVLTAAKLGADIATIPFKVIEQLARHPLTDIGISKFLEDWQKVQK
                     RV"
     gene            40115..40759
                     /locus_tag="PLM79_03605"
     CDS             40115..40759
                     /locus_tag="PLM79_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOV85421.1"
                     /translation="MRSRVGRVWALPGAALLFCGIMMGTPSSRAEIYRYVDRDGVMHF
                     TNVPTMPQYRRVPNLPQIATLRPNYYRLLPNRTSASSCESLNQYAYDPHIRLVCGRHG
                     LDDRLVKAVIKAESAFDPNAVSPKGAMGLMQLMPDTSRDLGVVNPFDPYENIDGGVRY
                     LKALLRRFNNDVRLALAAYNAGPESVQKHGGIPPYDETQIYVQRVLEYYGRYGK"
     gene            40840..41433
                     /gene="pgsA"
                     /locus_tag="PLM79_03610"
     CDS             40840..41433
                     /gene="pgsA"
                     /locus_tag="PLM79_03610"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOV85422.1"
                     /translation="MAAMTRFDFSRETLTNWPNVLTYLRMASIPLIILVLMPPPTGSA
                     HNTAFFLFAVASLTDYLDGILARRHNQVTSVGKLLDPLADKLLTSAVLIMLIPLGKVP
                     AWLVFLIIGREISITGLRSIAASQGLILNASRMGKNKMISQTIGLLFLLLYVPGIQDV
                     LDGTGLFFLWISLILGYWSAMDYFSGFYREATRHWRS"
     gene            41455..42558
                     /locus_tag="PLM79_03615"
     CDS             41455..42558
                     /locus_tag="PLM79_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85423.1"
                     /translation="MAFTLPPYVPPDFRQAALEEAPTVRFAPAPRDGVAPEGYHATSI
                     YPEYLKLQPGGWHLLRESRMDCVVVLGQDATPSVREFRHLKQGDPVACGREENGEEGI
                     LVHTTGFSAPGDLPEKFAFRTQVTRETSFSFDYDALYDLLEYERDHGFILWVLGPAVA
                     FDRDSRRALVQIIAGGHVHGLLAGNALAVHDIEAALFGTALGQEIYSKRQVPKGHYKH
                     LDAINTIRSLGSMEEAVTRNRIPDGIMRTLIDRKIPTVLAGSIRDDGPLPEVIADVYR
                     AQDQMRTLARRATTVVAMATQLHAIAVGNMLPSYHVTPEGRVRPVYFYSVDMSEFAVN
                     KLANRGSLTAHSILTNVQDFIVMLERGLRKRNS"
     gene            complement(42555..43517)
                     /gene="arcC"
                     /locus_tag="PLM79_03620"
     CDS             complement(42555..43517)
                     /gene="arcC"
                     /locus_tag="PLM79_03620"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOV85424.1"
                     /translation="MPKSKPVPGKPETLLVALGGNALIRKGQEGTIREQFENLVVPIR
                     QIARLSRTHRIVITHGNGPQVGNLLLQQECCSQVPRLPLEILVAQTQGQIGYMIESTL
                     DNELMKLGLDGKRFFVTLLSYVVVDEKDPAFQNPSKPIGPVFSREEALGLPYATVRTP
                     AGYRRVVASPKPVTIVEKREIRMLVDMGFIVISCGGGGIPVVKESRTFHGVDAVIDKD
                     LASAKLCEEIGVDVFVIATDVEGAALWYGTPRQRFLSRLNPEEARKYLQAGEFPPGSM
                     GPKVEAAIQFLDSGGKRAVITSIRHIEDAVAGKAGTQLTRGDSA"
     gene            43667..44944
                     /locus_tag="PLM79_03625"
     CDS             43667..44944
                     /locus_tag="PLM79_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85425.1"
                     /translation="MPQDRQWKKVLSWALYDWANSAFATTVMAGFFPVFFKEYWSAGA
                     GVTESTFQLGIANSGASVVVACMAPLLGAVADCGRAKKRFLFFFALLGILMTGSLYLV
                     AKGEWKIAVVCYSLGTIGFSGANIFYDSLIVGVARGRRLDFVSSLGFALGYLGGGLLF
                     ALNVFMTLYPRSFGLEDAAQAVRISFLSVSLWWAVFSVPIFLWVEEPLGTGNKTAGGW
                     AAVQGGMRQIASTFREIRQLRTVFLFLIGYWLYIDGVDTVIRMAVDYGLSLGFSSKSL
                     LTALLITQFVGFPAAILFGWLGERLGAKTGIFLGIAVYLGATLWAYFMDREIEFYALA
                     VVIGLVQGGVQSLSRSLYARIIPPGKSAEFFGFFNMLGKFAAVIGPALMGWVSLLTGN
                     PRFSIVAIALLFLSGAAILSFVHEGPQGTALSS"
     gene            complement(45085..45732)
                     /locus_tag="PLM79_03630"
     CDS             complement(45085..45732)
                     /locus_tag="PLM79_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV85426.1"
                     /translation="MGCRILLADDHKIVRDGLRSLLEKQPGMDVVAEADNGRTTVRLA
                     RELLPDLVIVDIGMPGLNGIEATRQIVAENPRIRVVALSMHSDRRFVAQMFKAGASAY
                     LLKDCAFEELAQAVQVVLAGRTYLSPQVAGTVVEDYIRHLAATDGSGFSVLTAREREV
                     LQLLAEGNSTKQIANSLHVSMKTVETHRQQIMNKLNVQSVADLVKYAIREGLTSL"
     gene            complement(45950..46267)
                     /locus_tag="PLM79_03635"
     CDS             complement(45950..46267)
                     /locus_tag="PLM79_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85427.1"
                     /translation="MERRRWIAVVLAMLFMSACLAAALDAGDAHARGSKSKTATLLGT
                     IYKSDTGYVLITGKTKYNLGGQDFSKFLGKKVKVRGIYTKGEKGRVLEVTKIEEAKPS
                     KKK"
     gene            complement(46453..>47151)
                     /locus_tag="PLM79_03640"
     CDS             complement(46453..>47151)
                     /locus_tag="PLM79_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOV85428.1"
                     /translation="RAFDEMAESLEQRALEMRTLASQLSLAEERERRRIATELHDRVG
                     QALAMSKIHLGALRETSKSPEQKAAVEQVRNLIEQAIQDTRSLLFRISSPILYELGFE
                     AALEWLTEQAQKQHGLDARFEDDKQPKPLDDDIRGLLFQAANELVVNVAKHAHAKNLR
                     IVARREEGFILVSVIDDGVGFEVPAGGKGWGRADGFGLFSIRERLGFVNGTLRIESRK
                     GEGTCVTLKAPLKP"
BASE COUNT         9580 a        14842 c        13915 g         8804 t
ORIGIN      
        1 cccgcaggct tgctcagctg ccgccgccca tccggatgtg tgatgcgctg agcgggaatt
       61 tgccgggaac cgttaaaacc atcctcgcaa actgcctgac tcatggccga cgcaactttg
      121 tcgacacgat ccccgccttt cccgaacaag ccgaatatgt catcgacatc ctcgcccgcg
      181 tttatcatat cgatgatccg gccaaggcca acaggctctc agccgatcag cgactcgcct
      241 tccaccagca acacagcgcc cccctcatgc aagacctcaa ggaatggttg gaaaaccaac
      301 cggcggaaaa ctccgttgaa ccctactcca gtatgggcaa ggccatactg tacatgctca
      361 accactggcc cgctctgacg ctctttctgc gggtccccaa cgccccaccg gataacaact
      421 tttgcgaaag ggtgctcaaa cagtgcatcc tgcaccgaaa aaactcactc ttcttcaagt
      481 cccttcggag agcatgggtg ggggacctct tcatgagcct cattgccacc tgcaggttca
      541 gcggcgttaa tcccttcgcc tatctcacgg ccctcctgca gaacacgaaa aagatcctgc
      601 aatccccggg ggagtggatg ccgtggaact atctgcacac cctcgccggg ttgccgccat
      661 aagagacgaa ccccgtgcct cagactcgcg atcaccgcac gctaaccagc ccccatttgt
      721 cctaaccgca ctgacctaac actgcgggga tccctgtggt gccgggagca cctttgcccc
      781 gattcagaaa aatttgcccg ctactgaaaa aattcggccc cgtgcaccct tgccggaaac
      841 acacgttgta ttcgggcggc ggcagtcgac tgtcttgtgc gccgtcgcgg agttttcctt
      901 ttcaacatcg cctccgcttc cgacgccgac ataggaaaag ccgtctttgt aaaggacgac
      961 cagacggtcg gcctggtggc ggaagtctcc aaccccattt acgtcggcaa tatcttcgag
     1021 gtcgagtcgg cgacgaaggt ctgggtggaa atttatcctg ccctgctgca aacggacgta
     1081 gccacgcaca tctccgatgc ctccggcgcg cacgcggcat ccgccatctc ggtagccgat
     1141 tccggaagca agatcaccgc aacggacgtg gaggccgccc tggccgaggt catgaccggc
     1201 atcaagacgg cgcagtacac tcttttcccg caagcaatca ctctcgagga cgggacggcc
     1261 gtcggcaaat ttacgagcga ggatcagcct tcggtaggat gggcgcagct ctccaataaa
     1321 gagctcgccc taaggggatc cgtacccccc aaatcgctca atccgggaag ccggcgtggc
     1381 cggtttttca tggatcatga gtctcagcgg aacgcggaac cggtttgatt cccctttcag
     1441 cgcactgggt gctcggggaa gctgcccgga gcaccccgtc tgcccggcac gccacccacc
     1501 ccaagaccac cgggcaggtc gacgtctcaa gaatcctttg ggagagctat tttcttcccc
     1561 gggtttctca gcaagaattc ttcctgttgg acggcgggat ccatcaggcc gatctcttga
     1621 cggattcaag tccagggatt atttagtccc gcaaattgtc gggaaccggc ctctttgtgg
     1681 ggaatggatc ccgaatataa cgcaacgtac tggattcaca atggattctt gcgctttctc
     1741 cgacggctcc tccccgagga cattggcggc acgggatgct gcagatccac aggacttcac
     1801 cgctcccaac gaaggatggt ctatggtctc aatcacggtc gacggaaaga caatcgaagc
     1861 ggaggaggga agcagccttc tccaggcatg cctcgatcac ggcatctaca ttccgaacct
     1921 ttgctttctc aaagaaatgg acaagcccac cgggtcttgc cgactctgca ttgtggaact
     1981 ggaaggcgaa gaaaaacctg tcgcatcttg cggcgtgaag gtcaaggaag gcatggtggt
     2041 tcgaacggac actccccatc tccggcagct ccagcgggtg gactttcagc tgatcatgtc
     2101 cgcccatcac atggaatgca agaattgcct ttcccggaaa aaatgcgaac tgcaaaagat
     2161 tgccaagacc ctggaagttc gtctcaaacc gacgaaattt gaggaactgg gcagggacct
     2221 ggccgttctc aagcaccctt gcttcgatct gctcccggcc cgctgcattt tgtgccgcaa
     2281 gtgtctcttc gtctgcgggc aaaacaacga gaagccgctc ttgaggctgg tgcgaaacgg
     2341 gtacgaaagc gtcatcgaag ccacatgcga gggggaggac ccggacaagc ttccctgcag
     2401 gtactgcctg gcatgcgtgg aggcctgccc cgtaagcgcc attgtgcccg ttgcccgtga
     2461 gttggacagg ggagtgtagg ggggattgga ccttcccctt ggacctcttg cgcccacggg
     2521 ggcggggggg ggggcgaatc ccatgccggg atccaatcgc caatggacgg gctcaagcgg
     2581 gttcctcaag ggttttcagg accacccggt tgcgccctct cttttttgct tcgtagagtc
     2641 cctcatcact cagggcgatg agggtttccg ccgggcgcgt ttcggtggga atcatgctcg
     2701 agaccccgca gctgatcgtc acgtagcggc gttccgaaga tgaggaatgg gcaatttgca
     2761 gggtctgaac ctcccggcgt atgttttccg ccacccgcac cgcccccttg gaatcggtgt
     2821 tcgaaagaat cacggcaaac tcttcccctc cgtagcgggc gaccaggtcc gtggggcggt
     2881 tgacgctttt tcggatggcc tgggccacca cacgaagaca atcgtccccc acttggtggc
     2941 cgtaggaatc gttgtagagt ttgaagtggt cgatatcgca gaggatcagg gacaggggac
     3001 cctgctcgcg cctcatcctt ctccactcct ggccgaggaa ctcatcgaat cttcgacgat
     3061 tcgccacctg ggtcaggccg tccagggtca ccaggcgatg caattcctgg ttcatctccc
     3121 gaagggccgc ctccacccgc ttgcgctcgg taatgtcgct caggattccc acggacccga
     3181 tggacttccc ggtctcgtcc cgccagggaa aggagtggtc tcccacccac ttcaggtccc
     3241 cgcttttggt cttgatgaga taatccgccc ggtattcgcc tgttttgccc tccttgcggt
     3301 ttcgggtaag ttcctccgtg gagagggtgt tgccctcgag accttcgagg ctcatgatca
     3361 gactggaaaa ccccagggcg ttgatctctt cgggggagta gccgataagc ttcaccacgg
     3421 aaggactgat gtagtcgaat ttcatgcaat gatacttgag gcggtacagg acgtccccgg
     3481 tggtctccgc cataaagcgg aaacgctcct cgctttcacg cagggcctgc aacaggcgct
     3541 ggtgttcgtt ttccagggat tccaggtcga ggactctccg acgcaacccg gccaattcct
     3601 cgaggagctg ctctcttgtc ttgtcctcat ctctcatttc gagcactgcg cgcacactcc
     3661 cgggaaccgg cgagggggat cgaacccttg cccgtttcct gcccggacat gcccccgtcg
     3721 aaccgagcgg ggacccgccg tccgggtcac tctttctgga aagtgatcct gcgggccgcc
     3781 tgagcgaccc cgatgggaac ggttttgctc ttggcaagct gtttgaggtc ctgctgcgtc
     3841 aaggtcggca ccagtttgat cgcgatgggc aggggcgttt tgggattggt caccagcgcc
     3901 atccggatgg cgtaggattt cacccattca cgattggatg cgattttccg aaggacttcg
     3961 tcatccacgt ttcgggaatt ggcgaaggcg gcgatttccc cgtcggtgat tcgcgggttg
     4021 ccgatgatgg ccgcctggac caggcgattc ggatcccgca tgagaaggga tcggcattcc
     4081 ttgtccccca tcatggcaag gcggaccttg tcgttcacct tcattttccc gactcgctgg
     4141 acgagggact cgggagtttg cgggccttcg gatgcgtcgc cgggggggct ttcgtcccgg
     4201 agtcgcatgg cctccaccag gagatgttcc cacggcttcc ggactacctt ttccacctct
     4261 tggggaccgg cactcatttg aaagcggccg tcccgccagg aaaggatgcg gtagaaggcc
     4321 tcctcccctg tcaggtcttc ggtgagtgcc tggcatacct gggccgagcg aatcagcaac
     4381 gtgcccttct tcgaccccgc ctccacctcg acccggcagt cgactcccga ggagcagatc
     4441 aactggatga ggtccaccaa ttgaatcccc cggagaagcc cgcggaatcc ctcgggctcg
     4501 aatccgcccc gtcccggagc atgctccggt ccggtccctg ctcccgcctt cctccggcga
     4561 tgcacggatt cgacgagaag gtattcccat cccttggaaa cggactggac cggctgttct
     4621 tccaacggtt tggtttcaaa ggaaccgtgc ccgcatccca ggagttcgta gacggcctct
     4681 tccccttgga cctcccccag gaccgcgtgg accacctgcc cggccgccac cgcaatctgc
     4741 ccggaaacct cccccacccg cacggcgaca cactggtcca accgggcgag acagagcagc
     4801 tggaccagtt cggtcagggc aatgccttcg acggtacccg aaaagcccgg cccgggggtc
     4861 tgttccactt ccatcctatg tgccatctcc tgatcatggg acggtcccgg ccgcagaacc
     4921 gatcaccccg gcaagcggca atcccgggaa gacatcttcc ccagggaaag cagggtcctc
     4981 cctgacaacg actcgaggtg gatttgacga tcttgaaagg cttgttcgaa tcgggatcca
     5041 gcggatccgg gggaacgggg gtccgttccc ccgaaggaag tcttccgcga ggtcttcact
     5101 cttccttggc cgcttcgatg atcttctcct gcaaagctgc cgggacctcc tcgtaatggg
     5161 acagcttcat ggtgaacatg ccccgcccgg ccgtcatgga ccgaaggtcc ggggcgtact
     5221 tgagcacctc ggcgtggggc acgttggctc ggatgatctg tttctttccc ttgctttcca
     5281 ttcccaggac cctgccgcgc cgggaattca gatccccgat gacatctccc atgcagtcat
     5341 ccgggacggt gatttccatc accatgatgg gttccagcag ggtgggcttg gcatctccca
     5401 tggccttctt gaaagccatg gagcccgcga tcttgaaagc catttccgag gaatccaccg
     5461 ggtggaagga cccgtccacc aggcccacct tgaaatcgac cacgggatat ttcgccagga
     5521 ccccttcgcc ggatgcttcc acaatcccct tttccaccgc aggaatgtac tgtcgaggaa
     5581 tcacgcctcc cacaatcttg tcctcgaaga caaacccctc tccccggggc atgggttcca
     5641 tctcgatcca gcagtcccca tattgccccc ggccgccgga ctgcttcttg tatttgccct
     5701 gaactcgggc cttccccttg atggtttcct tgtagatcac cttgggaagc ttgaggttca
     5761 cttcaacgcc gaattttctt ttgagtttct ccaccgtggc ttcgatatgg atttccccca
     5821 tgcccgagag gatcatttcc ttggtttcat cgtttcgctc gagcttgagg gtgagatcct
     5881 cctccatgag gcgggaaagg gacgagaaga tcttctcttc gtctcccttg ctcttgggtt
     5941 ccacggacag ggaatagacc gccgggggca actccaccgg ggcaaacaga atggcatcct
     6001 tttccgtgca cagggtgtcc tgggtcgtgg tgtccttgag tttggccacg gcgacgatct
     6061 cccccggccc ggcgctctcg atggggtcct gggactttcc cttgagtcgg agcagttggc
     6121 cgaaccgctc cttggtctgc ttggtggagt tgtataccgt ggaatcctcc gtcagggttc
     6181 ccgagaagat cctcaggacc gagagacgac cggcataggg atcgctgatg gtcttgatga
     6241 ccagggcact gaagggagca ccggggtcgg gctcccgggt caccggcgcg ccgctgggag
     6301 atttcccttc ccgagggcct cgatccagag gggaaggcat ggtttcgacc aggaggtcca
     6361 ggaggggttg aattccgatg ttgccgggtc ccgaaccgca aagaacggga accagtttcc
     6421 cggaaaccac cgaagccctc agtccttctc ggatctcttc cgtcgagagt tccccttcct
     6481 ccaggtattt ttccaggagc ccatcgtcgg attccgcgac ctgctccgtc agcatttcca
     6541 tatattccgc aacctcaccc gccaagtccg ccggcacgtc ctgcaggtcg aacttcccgc
     6601 tgtccccctg gccgtagatg taggccttgt tgctgatcac atcgaccact cccttgaagg
     6661 tctccgccgc accgatgggg attcccaggg gaacgcactg cttgccgaaa ttgttccgga
     6721 tgtcttccac ggtcttggca aaatccgccc gctcccgatc catcttactg ataaaaatca
     6781 tgcggggctg accgaattca tcggcaaatt cccacacctt ctccgtctga acccgcactc
     6841 cgtcgatggc atccaccacc accaggaccc cgtccgcccc ctgcatggac gttttggttt
     6901 ccgcaacgaa attgaaatcc cccggcgtgt cgatgatgtt gatctggtgt ttcttccagg
     6961 tgagggtatt gaaggccgta ctgatggtga tcttcctctt gagctcctcc ggttcgtagt
     7021 ccatggtgga agtgccatca tccacctttc cgagtcgcgt ggtcactccc gcgttgaaca
     7081 gcatggcctc cgtcagggag gtttttccgg caccgccgtg agaaatcgtg gcgaaagttc
     7141 gaaccctggc aacgtcctct ttcatattta ttcctttcta agatcattac cgacccaaaa
     7201 aaggtcgggc tttctggcgc tcgtggatcg ctgacctctc gggaaaggat acccggtact
     7261 cggagaccgg agaagggtga aaggtgcgtt caatcgtttg atattccgga aaaaatacaa
     7321 cgatggaaaa tagggcaagt caaaagagaa agtcaagaga attcccagga cgacggcccc
     7381 cgggaacagg ggccgggaag gccgtgccct gccgaaatct cggggccggg gctgcctctg
     7441 ttcccgagac cgcccaggga aacatccccc ccgcggcttg tccctccccc ggggggggaa
     7501 tcctaggaca cggggacctt ctccaggcgc cacaaggccc acagtcccgc cagtgccagg
     7561 agggccccga tcacgaaccc ttccgtcctg gtggcatgca tgaggaccac ggacagcacg
     7621 cccagggcgc cgcagagcag atagagcacc aggaccgctt ccctctgggt gcaacccaat
     7681 tggagcagac gatgggacag gtggtccttt cccggggaca cgatgggatt gagcccccga
     7741 cgcagcctgg atacgacgac cagcgtcgta tcgaaaatcg gtactcccag aaccaccacc
     7801 gggatcatcc aggtcaccac ggcgacattg tcgggaaatc gcagcttgat gccgatggcc
     7861 gcgaggacaa acccgatgaa caagctgccc gagtccccca tgaagatgcc ggccggattg
     7921 aagttgtaga gaaggaaccc cacgcacgcg cccaggaggg ccgccgccag gctgcccacc
     7981 aggaactgtt tgttcatgac ggccagcaaa aggaagaaag cgcacgcgac ggtggccgtc
     8041 cccgcggaaa gcccgtccat gttgtccaaa agattcagtg cgttggtgat gctcacgacc
     8101 cagaagatcg taacggcgac gttcagcacc gggtgcggca ggaaggtcac ctggactccc
     8161 gacaggagaa ggatgaccgc cgcacagacc tgcccgctca attttgccac gggtcccagt
     8221 acgcggtaat catcccagat ccccaggaag gatacgaagg tggcgcccag gagaatgctc
     8281 accagctggg agaggtaaaa gaggtcgaaa accgccaggg ccgccatgca acccaggtag
     8341 acggcgaccc cccccaaacg gggcagaggg cgaagatgca gtttgcgggg gccgggttca
     8401 tccacgaagc cccatcgaag agccgtcctc ttcaccaagg gcatcaccag gaccacggtg
     8461 aaaaaggcac aggcagcgat caggatgagc ttgatcatgg agagccccga tcctcctcga
     8521 gtccccgggc accggtacgg gccttcatct tgtcgatgga atcccggatc tttccgtgtt
     8581 cgctttcggg agaaagcttc cggatctttt ccagctgagc ggcggcgtcc tcgggacgtc
     8641 ccatcctttc gtaaatcaag gcaaggtttc ggtacgtggc gacattgtcc ggctgaagct
     8701 ccaacacctt gtaattctcc tccagggctt cgtggaactt gccccatcga tagtagatga
     8761 gccccaacag gctgtgggga cccaccaggc cggggttgat ctcgacggcc ttgaggttgg
     8821 cctcgagaga cgcccgctca tccccctgcc ggatgtagat ctggccgagg gtcgcccaca
     8881 gctcggcatt ctccgggtcg agggatacgg ccttgcggta cgccgccgag gcctccctcc
     8941 ccttctgttc cctgtggaac acctccccct ggagcaggta ggtgtccgcg aaacgatcgt
     9001 ccacggccag ggaagcttcg atcttctcat gggccttttc aatagccccc tggtacaggt
     9061 acaccctggc ccagttgttg tacagcacga ctcttcgggg gctgtttttc acggccaggt
     9121 cgtagaaatg atgcgcctgt tccagtttcc cttgcctttg ggcatccgag gaaaccgact
     9181 ccgcccattt ccaatacagc agggccaggt tcgccaggtg gtccgtattg agggggttca
     9241 gctcatgggc ccgggacatg gccttttcca cccgggaata aagagcgtcc ttctctcgat
     9301 ccctcgatgc accggctcgg tggaagtaaa agcgacccag catgaggtgg taatgttcct
     9361 gctcgggagc caggcggagg gcctccccaa aatgacgggc cgccggttcc cacttcttct
     9421 gtctctccag gtcccgggcc atcctaaaat gaatatccgc ctggaccgga cgaagattga
     9481 aaaccacccc cccccagcag gcgaccgccc ctacggcgac gtaaagcacc catttccagg
     9541 agttcgaaat ccattgctcc catggctttc ccgcggccag aatccctccc aggaccgttc
     9601 ccgcacccag caccgcccac gaatagaaga cgatgctctg cgcctcgctc cccgggcgca
     9661 taacaaggag gaacaaaccg gtcccgccca cgccgaacaa agccgaaagc ccccaggcgg
     9721 ccacccccga tagccgcgac cgaggttccc gaccctgggc ggattcggac cggtggatcg
     9781 cccaaagcag ggtccctgcc aggagccatg tgcccagaaa gaccgcaggc attccataga
     9841 ccgtgcgcac cccgcttccc aggcgcacgg tggtcacgga ccgccagacg gactcgaggg
     9901 tttcctccct taagaaaggg ttcagggtac aatcaaaggc cagggtgaac aaagcgaacc
     9961 ccagggccgc aaagcccgcc atcacggtgc gacggccaac caacctcgag aaagcacccg
    10021 actctccctc gatccttacg ggaaccttcc ccttctttcg cttcccacgt ttcccggcgg
    10081 acggaggccg ggccccgggc tccttggtct cgccccccac tccgggcgac gagtcctccg
    10141 gcaccacccg gcctgttccc aggaccacga ctacggcgca atagacccag aaataaaggc
    10201 gcgtggcggc cacggacacc ccggtttgaa tctccacgaa gtgggtgatg aggcccgaaa
    10261 ggagggcgat cagcaccacc tgccgctctc gcggcagggg agtttcctcc cggggccgaa
    10321 ctccgaaccc cgcaaccagg acggagacca cgactcccag gagcatcccg gcggggattc
    10381 ccagcccgag aagcgcgaag gaaccgtgga ggcgaaggga cacccaggtt ccccccaaca
    10441 ttcccaggaa cacgaggcca taaaacaccc tcccatggga agcaccgggc atcagcccca
    10501 gccactggat gccgtagcga aagacaagga ccaccaccag gagctgggcg gccaatcccg
    10561 cgagccccgt ggtcaccacg gcatccaggg tttcattgtg ggcacggtcc ggggcggcgt
    10621 ttcggtactc atagtgagcc agctccgggg gagcgtacgg gtcccacacg aatttcatgg
    10681 tctcgggtcc atgaccgaac cacatcctgc ccggattccc cgcgatcaac cgggccgtgc
    10741 cctgccaaac cagcatccga accttgccgg tcccggtttc cacatcggac aactgagcga
    10801 gacggccgag gtacggatag ccccggagag aatggagggg agaagcgggc atgttgagaa
    10861 ggaggaggaa caaggccgcc aggagcccgt gaaagacaaa ggagatccaa agccatcttc
    10921 gacctcgatg ggtaagcgca aagaaaacat aggcgggcag aacccccacg gggatactcg
    10981 ccagggcaaa aacgaaggcc ttgaagacct cttttattcg agcggccccc tccccgggtt
    11041 cggccggtcg caaggcaatc aagcccagca gaatgaagaa cgaaatcccg caaagcaatc
    11101 ccagccaggg gcctcgactt tgggtaaaca gaatgcacga gacctggagt cccaggagaa
    11161 tcaggaggag aaggagcgcc gcaacattcc agacccgaga gcccggggag cttcggaatt
    11221 tcatgtacct tcgaagcatt tcggccaagg tgacgggcac caccatgatc aggtaggctc
    11281 ccaagaagat gggatttccc atgttgccgc tgactctgcc ctcgatttcc ttttcccaca
    11341 ccagggggtc ccacccgaaa tgctgtgccg tcccatagag ggtgacggga atgctggtga
    11401 tgagcacgat ttgcagcagc cgctccaact gctcctgttg ccgaagagcc gccaacaggc
    11461 tgaaaaatac ggtcccgtag gccagggtcg tgaaaaaccc ctgggcgcgt tcgtaggacc
    11521 cccacaggct gtgggcgggc agcacggaga gggaggtgct gagaagagtt gtcgcgagga
    11581 agaggaccac gggcaggacc aggggtaccc gaacggcttg cttcaaggct tcccagggcg
    11641 ttgccgatgc attcgccccg gcagtcgagc gccccgatcg gacggaaccg ggcaggactt
    11701 cgattccctt cgccagccac agggccgccg acaccaaggc gatggagcga aagaccagca
    11761 gtttgtcggg ctcgaagatg cggctcgagt gggggttgaa aaacaaggga accaccatca
    11821 ccgacaacag ccagcccgct tcgatggcgg aatcacagta gacgacgaat cgactgggca
    11881 cgatgtggac ccctttatcc gcggccgatc cgggccgaag gcgcacgttc cctgggccga
    11941 agctctccgc ttcaagccct caattgcata tcatagaatc ggcaaacgga gtggaccatt
    12001 tttgtcgcgg agaattccgt cagtgctcga acccggcccc gggagcccat ctgccgccgc
    12061 agttccccat cctcgagaag gacggacagc gccgccttca gggacacggg atccccggga
    12121 ggcaccacga cccccgtctt cccgtgcacg ttcacgaagc tggttcccgt tcccagctcg
    12181 gtactgatga cgggaaggcc gcttgccatg gcctccacca gcaccagccc aaaggcctcg
    12241 ctccggtggc tcgaaggaag caccaggaca tccgccgcct ggtagtaagc gggaagcatc
    12301 tcctgggtta cctctcccag gaaggcgact ttgtccccga tcttcatgga aagaaccttg
    12361 tcccgcagtt tccgatcgag ggggccctcc cccaggacga ccagccgtgc gtccaggtct
    12421 gcaacggcct cgatgagggt ttccagtccc ttgtaatagc gaagcttccc tacgaacaaa
    12481 accgtggggg ccgcaaagaa ttcccggagg agacggacgc gggaagggta ggacctcaga
    12541 aacggtttca ggtcgatccc caggggaatc accgcgcact tgtcccgaaa ctcccccagg
    12601 tagggggacg actcgaggta ggcgccactg gtgacgatga tccctcgggc ggagctcaat
    12661 acccggttca tcatcgggcg gtagaaccgc aagagggtct tctgacggat gatgtcgctg
    12721 tggtaggtaa cgaccatgca gggcgcctgc ccgaaaagca gttgcgaaac ctctccccac
    12781 gggtaaggga aatgaaggtg agccaggtgg gggcgcaggc gtctcaagat gcggggcatg
    12841 ggaaggctga tgggagtcga agccgccgtt cccaggcgtc cggcccggat gagccgcacg
    12901 ccgtttcgag tctcctcttc gctcagtagg cccggactcg ccgtcagtac cgtaacgtcg
    12961 tagcccatcc gggcctgggc ctccgccagg agccgcacgt ggagctcgat ccccccgagc
    13021 acgggcggat aatccttgta gatgtggagg acgcaaatcc ccggacctcg atgcgccacc
    13081 gattcctttc cgaatccagc caaccgcatt ccgggggttg gccatagttc cgaaggtgtt
    13141 ttcaagggcc caatcgccgt ctcgatcccg cctccgggag acgcccctcc tcgtagtgcc
    13201 ggatgacgtc ggtcaaaatc ccgcgaaggt cgtaggacgg atggaagccg atgaggtctc
    13261 tcgccttggt gatgtccgga accctccggg gcatgtcttc gaactcctcc ccataggctt
    13321 gatcataggg aatacggacc accggcgacg ggctccccgt cagctgaaga acctggcggg
    13381 ccaactcatc gatggtgacc tcctcctggc ttccgatatt gaacacgtcc cccacagcct
    13441 cccggtggtc catgaggccg gccagggccc cgacggcgtc ccggacgtga agaaaacacc
    13501 gggtctgatt cccgtcgccg tatatggtga tggcctcccc acggagcgcc tggcaaacaa
    13561 accgcgggat caccattccg taggcccccg tctggcgcgg acctaccgtg ttgaagagtc
    13621 gaacgatgac ggtgggaagc cttttttcct tccagtagag gtaggaaaga atttcgtcca
    13681 cggccttgga ggtggaatag gcccaccggg atttgagggg cgagcccagg atccggtcgt
    13741 ctccttccct caagggagcg ttcgagttct tcccgtagat ctcggagctc gaggtcacca
    13801 ggactttctt gcggtaccgg tgggccacgt tcaagaccag ctcgcttccc ttgatgttgg
    13861 tgatgaggga ttcccacggg ttcttgacga tcaactcgac gcccaccgcc gccgccaggt
    13921 ggaacaccgc atggcagcgc tccacccaac gatccaccag gaattcgtgg aggatgcttc
    13981 ccaccacgat ctgaaacccg gggtggtctt cgaggtgaga cacgttttcc cacgaacccg
    14041 tggacaaatc gtccagaacg accacctcat ggcccctgcc cagcaggtac tccgccaggt
    14101 gacttccgat gaatcccgct cctcccgtaa tgagaaattt catatggagt gtttcctgta
    14161 gatccgaatg gtttcccggg ccgttcgtgc ccaggagaag gccgatgccc gatcgagtcc
    14221 cctgttccgc aagtcttccc gcaaagccgc atcctcgagg acccgccgca gtccctccgc
    14281 gagggtccgg tattgccggg ggtcaaagta cagcgcggcg tctccgcaca cctcggggag
    14341 gccggacgtg tgggcgcaca acaccggggt cccgcaggcc atggcttcga gcacgggaag
    14401 gccgaaacct tcgtacaggc tgggaaagac aaaagcccgg gcgcccgaat agaccgaagg
    14461 cagcaagtcc tcctccaccg gccccacgaa cagcacctcc ccctggaggc cctcacgggc
    14521 cgcatacgcc tttgcctggg gataccgctc gtcccagggc ccggcaagga ccagccgcac
    14581 gtcgtccccc gaggaccgag cttcccgccg gaaatccctc catgccttca gaagggtcac
    14641 caggttctta tggggcttat tgatccctac atacaagaca tacttttccg gaagaccgac
    14701 ccgcctgcgc atcccttgga tctcgtcccg cccccgaggc acaaaccggg aatccacccc
    14761 caggggaatg acggagatct tctcgggccg ggcagggaag aaacgcagca ggtccgagcg
    14821 ggtcgaatcg gatatggcga ggatgtgtcg gcatctttcc accagcaccc ggtgggcgat
    14881 gcggaacacc actcgctgcc atcggggaaa gaaccggggg aaaaccaggg ggatcaggtc
    14941 gcagcaggtc cacacggtgg ggatgtctcg aaaagaaggc gccgggtagt aagtgctgtg
    15001 gtagagggtg atgccgtttc gcctcaaggc gggcttcagt ttccagtgtt gggagaggga
    15061 aaagggggac cagggagccg gaatgcgggc ccattgaggc ggccgccacg acagggtatt
    15121 ccgtggatcc gccaggagcc gcaccggcag tcgggggtcc gcctcccgta tccccgagga
    15181 caggcccgcc acgtagcgac cgattccggg aaaatgttca gtggcggttc gagcatccag
    15241 agcgacgcgc atggacctcc ttgtagacct cgagagtctt ttgtgccgta gctgcccagg
    15301 tgaagccccg agcccggcga aatcccctcc gtcgcagttc ctgccgcagc cccgaacggc
    15361 tccaaagggc tcggacggcc tcggcccacc cggcaacatc ctcggcatcc acccgaaggg
    15421 ccccgtcccc gaccacctcc tcgagggcgg ccgctttgct gcacaagacg ggagtcccgc
    15481 aggccatggc ctcgaggacc ggcaggccga acccttcgta caggctggga aaaacaaagg
    15541 cccttgccag ggaatacaac cccggcagat cctcttccgg gaccggcccc agggaaatga
    15601 cccttccgtc cccgtacact gcggccggat cgtcccggat ctcccggacc ccccgtcccg
    15661 gggggcccgc caggaccaag gtcggacgct catcccccat ccatcgaaga gcctccagga
    15721 gcacctgcag attcttgcgg ggctcgaggg cccccacgtg gaggaggaaa tccacgggca
    15781 accggtaccg atcccccaga gatcggcgca cagccccagg gtcccgtgga gcaaatcgtt
    15841 cgctcacccc caggggaacc acgtgcactc tttggggagt cactccgtag agccgctccg
    15901 catccattct tgtggaattc gagacggcaa tgagggcatc ggcccggcga aggaaatgcg
    15961 gcatggcgag cttcaggtac aggcgattga ggggagccgt cgtacgggga aagagccgga
    16021 agacaaaatc gtgaagagtg agaacggttc cgacccggcg gaaggcgggc agcaggttgt
    16081 ccgttccatg gaaaagatcc agaagaggaa accggcgctc ctgggaagcg cccaccatgt
    16141 gagccaggag tgtttgcagc cggaaagccc tttccgcgcc cgccgcgttg gggggacgac
    16201 tcccggggcg ccgtgggacc accgtagggc acccgctccc cacggacagg ggtatgatcc
    16261 gggtttcccg gtccacgtcc ccgagggcgg tggtcaactc ccgggcgtac cgagccattc
    16321 cgccgggccc ccgcaggacg gccgtgacgt caatcccgac tctcattgcg gttctcgcat
    16381 tcgaatccat gaatagcgcc gccgaatccg ttgcgcgggt tgcgtcgccg ttgggtccct
    16441 tcggctagac catggtcctc ttggcagacc ggacgttgta cagggcctga ttttcgcaaa
    16501 cgtgaagact gcccgattcg tccatatcga gtttctcaaa agacaaatgg gccttgtaga
    16561 acatgttcag gtttgcggtt tccgccttga gatagcgagc ggtcgcccgg caataatgat
    16621 cgtctatttc tataccgacg ctgtttcgac cagaccgcag agccgcaatg agggtggtcc
    16681 cggtgccgca aaaaggatcc aaaaccgtat cgccgacgaa agaaaacatc cgaaccaatc
    16741 gtgaagcaag ttccaggggg aaaggtgcag ggtggtcttt tgtcgaagcg ccggtgatat
    16801 gccaaatctg tctaaaccaa ccgtcaaagt ctttcttctt gatcttgctg agttcccttt
    16861 gtttctcggt tgggcgcctg tatcccccgg gcttcctttg catgaggatg aattctatat
    16921 cgttcttgat gattgcattg ggttcgtagg gcttcccgag gaacttgcat ccgtttgcga
    16981 cctcgaaggt agcattcgag atcttgtgcc agatgatggg attcaagttg tcaaaaccaa
    17041 tcttcctgca ggatacgcaa atatccgaat ggagtgggaa cacaagatgt cggccgaatc
    17101 tccggcggga gacacacacg tccccgacca cacacaccag acgccctccc ggcaccaaaa
    17161 tacggaagag gtgacgccat actttctcga gctcggacag aaacgcttcg tagtcttcaa
    17221 tatgtcccaa ctgatccgga tgttcgttgt agcgcttgag gttccaataa ggaggagagg
    17281 ttaccaccag gtgaacggat tcgtcgggaa gaaaggatag atccctcgca tccgcatgga
    17341 tcagtcgatg ccgtgtttcg cccgcttggc tcattgtgcg aatgctccta cgtgggacag
    17401 gatggatttt gcgaacctct caaaagaaag gtccttggcg ggttcatgat actcgcctct
    17461 caaacccgcg tcactgcggg aagtcaagaa gcaggccgaa gtataatgac gttccagaac
    17521 cagctttcga caaagcaatt catacctctt tgcgtaggat gcaccgacaa actccggaaa
    17581 gacattgaag tgcggctcct tgacttttac aggccgatgg gaagccgggc actcttcaag
    17641 catgaaaaaa taacccaaga acggctgcgc ccctgcattg aaagcccctt ctccgaaggc
    17701 cgtccaaaga tcgagggcac ttccaacggc ctcctcggtt ctgttgttga agttgttgcc
    17761 gaatgagggt ccgacttgtg atttggcttc gattgctgca accagggtgc cgtcccggac
    17821 aacgataaga tcccattcct tggtcggtcg aaaataaccg ggcaactcca gggctgtctt
    17881 tcgaaagatg aacttcatcg ggaggcctgt ttccgtgatg agtgcagtaa acaaatcaat
    17941 aaaaccgtcc atctgcgccc cgcccgtcac ggcacttctc agccccgcat ccgattggcc
    18001 gcgcaactct tgttttttcc tctgtgtttc cctggtagcc caatagtgag ccactgcttt
    18061 tcccacccgg cccgacagtt tctcgagtat tttctgcacc gcagcccttc tcacgttctc
    18121 gggaattgtg cccaaatacg acgaccgaaa ccagtggttt cactcggcag attcgcccct
    18181 aatattatcc ggaaatggtg ggactttggg aagatttcca tcctctgctc gtaagaccgg
    18241 cccaaatacc gcatgggaaa acgcgatccg gcatttcgct ttctcctgtg gcgatatcgt
    18301 aaagattcga cgcggaaggg acgaccctct ccgccgtgca accgcgcggc atccccaccg
    18361 aggcactctg caaacccaca agggctgatt tttttccgca aagttgcgtc aaagaagcga
    18421 atcatagacg gcggcgacct gtccggcggc gtcatcccgg gaaaaggtgc gggcgcgatg
    18481 cagacccagg gtgcgcaagc gttcccgcag ttcgcgatcg gtgagggccc gcaggattcc
    18541 ttcggccagg gagtgggctt ccccgacggg cacccggatc gccgcatccc ccaccacttc
    18601 cggaaggctc gaggtttccg tacaaaccac cggaacgccg caggccatgg cttccagggg
    18661 cggcagcccg aacccttcgt accgggaggg gaagaccagg aggccggcaa ggttgtacag
    18721 gttcgcaagg tcccccgggt caatgaagcc cttccaatgg accctggcgc aaaagtccgc
    18781 ccgacggagc tgtcccggga gatcctcgac cccccaaccg gcccggccca caaggaccag
    18841 gtggtggtcc gtttcccctg caagcaggcg gaaggcctcg agcaggacgt ccaccccctt
    18901 acggggttcc agggtgccca caaaaagcag gaacttcctg ggaagccggt atttggccgc
    18961 ggtcctctcg aggcgggaag catcctcgac cggccgaaac tcctccccca ccccgggata
    19021 caccacgtgg atcttctccc cgggaacgcc cgtcaggcga aggatgtccc ggcgggtgga
    19081 atgagagctt gcaatgaccc ggtcggccca ccggatgctc ttgggaagcc atgagctcca
    19141 gtaccatcga gaaaccggcg gaaagttccc gggaaaaagt gctccgatga gatcgtggac
    19201 cgtaaggacc gtgggacagg gggcccaccg gggcgcgtcg aagcccggaa tgtgaagaag
    19261 cctcactccc gcaattcggg cttgcaaagg caacaggacc tgctgccaca ggagtcgccg
    19321 gtgggtgcga aagtccccgc tcctcccccg ggagatcggg aggcattcgt attggcgggc
    19381 gacccggcgc aacccggcca ggaggttttc cgtatacact ccgatccccg ttttccgccc
    19441 ccgggtcgac tgaacgtcta tgccgacttt ggcccttttc aacaccgctc caggatgatt
    19501 cgttcggccc gccggacgtt ttctcccgca tcgtagcgat ccaggacccg ggccctgcct
    19561 gcggctccca tgcgccgacg cagatcctcg tcttccaaaa ggtccagaag cgccgaagcc
    19621 aatccgggca cgtccccttc ctccaccagg attcccgcct ccccgtgggg aacaaattcc
    19681 gggattgccc cgcttcgggt ggagaccacg gggattgcgc aggccatggc ctgcaggttc
    19741 gtcatgccca cgtactcttc ccatccccgg gtagtccggg acggcgacgc caggacagcg
    19801 gcactgcgaa aatagggagg cagatccccg tgggggatgg tccccaagaa tcgcacggcc
    19861 ctttccggaa gccgcgatag cgcccgtcgc cgcgcctcgg cccggtgagg tccgtccccc
    19921 aggatcgcca ggatcgcccg ggagttcttc cggtacactt gttggaaggc ctcgagaaga
    19981 tcgaaaatgc ccttctgccg gtggactcgt cccacaaaca acaccaccgt cccttccccc
    20041 caggcgggtt cccggccgtc ccgttcgggg ctgaactcct tggggtccac cccccaggtc
    20101 ccgtacatgt gccggaccgt tttggaagcc ggaaccccca tccactcgag gtttttcgag
    20161 gccccctcgt tcacatggaa aaacagccgg gtccttcgca ccacaggctg aagaagagct
    20221 cgcaggagcc gagccgggac ccgcccgtac ttttcttcca ggggccgggc cacgtggact
    20281 cccgccacca ggggaatcct tcgaagaacg gcgtaaaaga agatcgcagc cgtgtaagga
    20341 agctgcccca gggataggat ttccacggtt tgaagaatgt ccggtttctc ccgccgcagg
    20401 aggcgcaaaa gatccagggg gcccttgaag gggatgaggc tttcgtccca atcggacgaa
    20461 cgcagatcgt tgtagggggc tcgccggaag aaatgccgga tcctgagctg ttcgaaacgg
    20521 tgcatggggg caatgaggtc ccgccggatg tccatgctga ccactgccaa agtcccggtt
    20581 ctgccggggt gaacacccat gcctagtcct cccggtcccg cccccgcctt cgccttcctg
    20641 cggcggccag ccgttccacc aaccggactc cccaggtccg tttgctcaaa taccggtagt
    20701 gcagcaccag gaagcagatg cgggccaggg ttgcgacggg gttgccccct tccctgaagg
    20761 cgcaccggaa atgatgcccc atcctcgaga tctcgcccgc ggacagccac cgcaacccca
    20821 catccatgtg ggcgtttcgg taggcaaggc ccttgacctg caggagttcg gctcccaggt
    20881 ccttccggcc gaaagcctta tccagggtcc ggaacctccc ccgggcaacg cgatcaaaaa
    20941 ggggagatcc ttttcccgtg gtgcgatggg agtgcatcct gaagtgggcc aggacctcgt
    21001 ccaggaaatg aaacttgcag acgggcgcca ggcggatcca catgtcccag tcctcgttgc
    21061 ccgaaaggga ctcgtcgaaa ggtcccgctt cgtccaggca tcgcttccgg accagggtcg
    21121 tgatggtaca gacgaaatct ccgtagagaa ggctcttgag agtctccccg gggtatcgtt
    21181 cggggactcc caggacttgg gccaggggtc gtcccgtttc gtccatggcc tgggcccggg
    21241 aatacaccaa cccgacttcc ggacgctttt cgaggattcc catttccttt tccaggagat
    21301 tcggcagcca tcggtcgtcg gaatcgatgc gggcgatgat ctctccccgg gcgtgggcca
    21361 aagcccggtt caacgccccg ctgattcccc ggttttcctg gcggatcagg cgaatccttt
    21421 catcccgaaa ggcctccatc acggcgggag tctcgtccgt ggagccgtca tccaccacca
    21481 ccagctcgaa atccccccgg gtctgtccca ggacgctttc cacggccgtg ggcaggaaat
    21541 gagcgcgatt gtaggtgggg agcatcaccg tcaccggggg cgttttcacc gtgagcctcc
    21601 ctccccccga accaggcgcc gaagggtggc gagccgctcc agcatccgcc ggggtgccag
    21661 ggctagggaa aaacccaggt aagccccnnn nnnnnnncga cggctccctt gaccaacact
    21721 ttcccggcaa gagacagggt ttcgagcccc acccggtccg aaaagaccca ggagaccccc
    21781 agggccaggc atgccgccag tgcgggcagg accacggtat cggccgggga cagctcgagg
    21841 gtccgaaaca gggggcgata cagcagcggg agactcaccg cgtacgagat ccccaccccc
    21901 aggcacgtcc cgacggctcc gtaggcaaga gtcaagggaa cggcgctcag ggccaacacc
    21961 cccgcctgga tggccgtcat tcgggccggg agtccgggac ggcccacggc cataaagaag
    22021 gaccccgcat tgtcctgaag aggccggagc accgaaaggg ccaccaggaa ccggagaagg
    22081 agggcgctgg gaacccatcg ggggccgtag agcagttcga ccaggtcccg cgcggagacg
    22141 aacaccgcca gggccaacgg cagggccagg gtcgccacga gccagacggt caacatgacg
    22201 acccttcgca gacgctcggg atctccctgg aagcgggcgt aggtgaagaa tcccgctcga
    22261 tggatcacgt tgcccaccag gaggctgggc cacatggcga tgcgccatgc ccggtcgtag
    22321 aatcccaggg tcgcaagtcc caggaaggtc cccaccaggt agttgtccag ctggttcacc
    22381 aacagcccgg ctatggaagc caggcccacc acgcctccga accgcaccag ctcccgggcc
    22441 gtcctcccgt tccatcgcca tggcaggttc cagagctccg gcagttttcg ccgcgccgtc
    22501 caccacattc cccccaggag aagcagggcg taggcgccgt tctggaccac gatgctccag
    22561 tagccgcccc ctttccacgc aagccacagg cccgggacat agctcaaggg gaatgccagg
    22621 ctcgagacca ggctcgtttt ggagaaatgg agctccttgt ccagcatgac ccaggcggtg
    22681 cccaggaccg agtcggagat gccgatgatc cccagggcca acgtcgccca ccccacctcg
    22741 accgggtact cgaaccaccc caggacgggc agggacaggc tgacgattag aagggtgcac
    22801 agccccgccg taaggtcgag ggccaggtga gttccgatca gttccccgtc ggtttccctg
    22861 cgctgggcaa aggcctgccc gatcccgatc ctcgggcgca ggttcaacag gctgaagaaa
    22921 aacatcccca gggcgaagat cccgaaatcc tcggggctaa gaagacgtgt gaggacaatg
    22981 tttgccagaa acccgaagac cacattgaac caggaactca aagcaaccca gattccccct
    23041 cgtgccaccc gggttcccaa agactcttcc tcggtccgta cgggcatgcc ggatgccgtc
    23101 ctttcccacg cagaaggccg taactcccga aacaaagggt ttccccggac cggtttgccg
    23161 acccgaaagt cccgccatgg taccacgaac cctcgtcttt cagaagccaa acacccccta
    23221 gcgaaccaag catgaggcca gtttgcaatt gattttttcc ccccgtgtgc tagcttaacg
    23281 tccttgcctc tggggatcat ttcggaatca ctccaaaaac gggacttgtg gtgcggggaa
    23341 gcatggatcc gggagccgcg acgaaaccac tcacatggac ggaatcggtg gagcttttcc
    23401 gaacgtacct gcgcctggaa cggggcgtga gcccgcacac cttgagggcc tatcagtcgg
    23461 acctggaaca gttccacgaa cacctactca cgaaaacagg gtccccttcc gtctccgtgg
    23521 acgagatcac ctcggacctg gtgcgaagct ttctggccgt gctccactcg aaggtggaaa
    23581 agaccagcca ggcacgcaag atctcggctc tccgatcctt cttcggcttc ctgcacgaga
    23641 gaggctgcac ggacaagaac ccggcctccg gcatcactca tcccaagatc aaatcccgga
    23701 tcccctcttt tcttacggtg gatgatgtgt tccatttttt ggattccctg aggcaatggg
    23761 ctcggactcc cggcgcttcg tggaggagaa gccggaactg ggccctctat gagtgcctct
    23821 actcgacggg catgcgcgtg agcgaggtcg tggggctgaa cgaagaggac cttttcgagg
    23881 cgcggggaat ggtcctggtc ctgggcaagg ggaagaagga acggatcatc cccgttggga
    23941 agaaggccct ggaggccgtc gaccaatatc ttcggaccct ggaggagcag tctcccgggc
    24001 ttcgaaccaa atccccggcg ctcttcgtca atgcccgggg ggatcggatt tccacccggt
    24061 cggtccatcg cattctcctg gcggaattga agcggtgcgg attgtggaag ccgttgagcc
    24121 ctcacggcct tcgtcattcc ttcgcgaccc accttctcaa ttctggggcg gatctccggg
    24181 cgatccagga aatgctgggg cattcgagcc tctccacgac tcagcgctac acccacgttc
    24241 acctggagca actgatgaag acgtacgacg cggcgcaccc gagaagcagg agaaaatccc
    24301 cgtgacgggc ctcgagggga ccgaaacgaa gcgcaggaat tttcccacaa cccctaagga
    24361 tgaacggagg gagtcgcttt cacatggcag acacgctctt tcgaggaacc accgtactgg
    24421 cgattcggca caacggtcag gtggtcgtgg cgggggacgg ccaggtgacc ctgggagaca
    24481 ccgtggtcaa acaccaggcc aagaagattc gcaggcttta ccacaacaag atcctcacgg
    24541 gctttgccgg ctcgacggcc gatgccttca ccctcttcga gcgcctcgaa gccaagctcg
    24601 aacagttcaa cggaaacctc aaacgggccg ccgtggaact ggccaaggac tggcgcatgg
    24661 atcgggccct tcggcgcctc gaagccctgc tcatcgccgc cgatgccgac cagtgctaca
    24721 tcttgagcgg cacgggcgac gtcatcgaac ccgacgacgg actggcggcc gtgggttccg
    24781 gggcccctta cgccctggcg gcggcccggg cccttacggc caacacccag atgtccgccc
    24841 gggagattgc cgaggaatcc atgaaaatcg ccgcgtccat ctgcatctac accaaccggg
    24901 aattcaccgt agaggagctg taacctcatg caacagccgt tgacccctgc agaaatcgtc
    24961 cgggaactgg acaagtacat catcggccag aaagacgcca aacgcatggt ggccattgcc
    25021 ctgcgaaatc gctggcgccg acagcaggtg cccgagcacc tgagggacga gatcgccccc
    25081 aaaaacatca tcatgatcgg acccacgggg gtggggaaga cggaaatcgc ccgccggctg
    25141 gcccggctgg cccagtcccc gtttctgaag atcgaagcca gcaagttcac cgaggtgggc
    25201 tacgtgggcc gtgacgtgga atccatgatc cgggacctca tggagctggc cgtgagcatg
    25261 gtccgcaccg aggagatgga agcggtcaag ctgaaggccg aagaaatcgc cgaggagaag
    25321 ctcctggata ttctgctgcc tcccaagaga cagcccgaga accgggaaca gcttctcatc
    25381 gagcccgacg cccggcaggc ggcccaggaa gaatccgacc aggcggactc gacccgggag
    25441 aaactccgaa agctcctgcg ggctgggtcc ctggacgacc ggtacgtgga aatcgatgtg
    25501 cccgaccgga attttcccat ggtcgagatc ttcgcgggct cgggaatgga ggacatggat
    25561 tacaacctgc gggagatgct gggatccatg cttccccgca ggaccaagcg ccgcaaggtc
    25621 aaaatccccg aagcccggga gatcctggtc caggaagagt cccagcggct catcgacatg
    25681 gacaaggtga tcaagtcggc cgtcgagcgg gtcgaacact cgggcatcat cttcctggat
    25741 gaaatcgaca agatcgcggg gagggaatcc gggcgaggac cggacgtttc ccgggaaggg
    25801 gtccagaggg accttttgcc catcgtggaa ggttccaccg tgaccaccaa gtacggaatg
    25861 gtgcggaccg accacatcct gttcatcgcc tcgggagcct ttcacatctc gaaaccctcg
    25921 gacctgattc ccgaactcca ggggcgtttt cccattcggg tggagctcac ttccctcacc
    25981 cgggacgatt tcgttcggat cctcaaggag cccgaaaacg ctctcatcgt tcaatacaag
    26041 gcgctcctga ccaccgaaga ggtcgagctg atcttcgagg acgacgccat cgaggagatc
    26101 gccgaaattg cctaccaggt gaactcgcgg accgaaaaca tcggggcgcg gcgcctgcac
    26161 accatcatgg agaaactgct ctcggagatc tctttcaacg ccccggacct caagggccaa
    26221 accatccaca tcacccggga gaatgtccgc cagaccctga tggaaatcat caaggacgag
    26281 gacctgagcc gatatattct ctaggtaagg acgagcgttc atggaggcat ttttgaagga
    26341 ccaatgcacg gtcattgaaa aggcgcggat tctcatcgag gccctcccct acatccggcg
    26401 cttttcccaa aagaccctcg tgatcaagta cggaggccac gccatggtgg ccgacgatct
    26461 caaggagagc ttcgcccagg acattgttct catgaagtac atcgggatgc acccggtggt
    26521 ggtccacggg ggcggccccc agatcgggca gatgctcgag cgcatgggca aaaagagcga
    26581 tttcctggcg ggaatgcgcg tcaccgacca ggacaccatg gacatcgtcg agatggtgct
    26641 ggtgggcaag atcaacaagg aaatcgtgtc cctcctgcac cgccacgggg gtcaggccgt
    26701 gggcttgagc ggcaaggacg ggcatctcat cgaagctcga aagctccatg tctatcgcta
    26761 ccaaggggac gacgagccgc cggagatcct ggacatcggc ctggtgggcg aggtttcccg
    26821 ggtgaacacc cagatcctgc gactcttcga acacagccgc atcatcccgg tgatcgcccc
    26881 cgtcggggtc ggagaaaacg gcgagacctt caacatcaac gcggacctgg tggccggcag
    26941 cgtggccgga gcgctccagg ccaagaaact gatcctcatg acggacgtac ccggggtcct
    27001 ggacgcccgg ggggagctca tctcgagcat gaccgtggga gaagccgcag acgccctcca
    27061 ggacgaaacc ctccggggcg ggatgatccc caaggtccgg tgtgccatcg acgccgtcca
    27121 gtcgggagcc gagaaggtgc acatcattga cgggcgggtt ccccacgccc tccttctcga
    27181 gatcttcacc gacgcaggca taggaacgga aatcagcaag aaaaggtagg aaagccgtgg
    27241 ttgccccggc catcctcgaa ggagcctggc atgaacgata ctcaaaccgc aagccgccaa
    27301 cacatcttca acacctacgc gcgactgccc gtcaccctgg ttcgagggga aggatgccgg
    27361 gtttgggacg atacgggaaa ggaatacctg gatttcctgg ccgggattgc ggtgtgcaac
    27421 ctgggtcatt gtcacccggc cgtgactcgg gccctgtgcg aacaggccca gcggctggtt
    27481 catgtctcga acctttttta tacgcgcccc caggtggaat tggccgcgga attgacacga
    27541 ctgtcttttg cagataaggt atttttttcc aacagcggcg cagaagccaa tgaagcggcc
    27601 atcaagctcg cccgaaaagc cgccaaagaa cgacacggcg aagggcggtt tcacatcatc
    27661 gccatgaaag actccttcca tggccgcacc atggccactc tttccgctac gggtcaggaa
    27721 aaggtgcaca agggtttcga acccctcgtg gagggattca cctttgtgga attcaattcc
    27781 gtcccggcgg tggaagcggc cgtgacggaa aagacctgtg ccgtcctggt ggaacccgtc
    27841 cagggagagg gaggagtgcg ttttccccgg cccggatacc tggcggagtt gaaagagctt
    27901 tgtctcaagc acgacctgct cctcatcttc gatgaagtcc aggtgggaat gggaagaaca
    27961 gggaccctat ttgcccacga gcaggaagga gtgacacccc atatcatgac cctggccaag
    28021 gccttgggaa acggtctgcc catcggggcg atgctggccg tcgaggaggt ggcggcggcc
    28081 ttcgtgccgg gaagccatgc ttccaccttc ggcgggaccc cgttggtcac ggcggccgcc
    28141 ctcgaggtgg tgaaggtcct ctcgaacccc gcgttcctcg gaggggtacg agaaacgggg
    28201 gcctattttc gagaaaagat cgaggcgctt cggccccgac acccggtcat ccgggaagtc
    28261 cggggaagag gactgatggt gggagtggaa ttgaccgtgc cgggtcagaa ggtggtggag
    28321 ctctgcctcg agaacggcct catcatcaac tgcacccatg aaaacgtttt gcgcttcgtt
    28381 ccaccgctga tcgtggaacg acaggacatc gaccggtgcc tgtccgtgct ggaggaagcg
    28441 ctgggaaagg ggacgtcatg aagcgggacc tgctgaccct ccgggatctt tcccccgaag
    28501 agatcctcta tttggtctcc cgggccaggg agctcaaagc cgccctggcc cagggggtgc
    28561 ttgctcccac gtgtgagggc aagatcctgg ggctcatctt tgtgaagccg tccacccgga
    28621 cccgggtatc cttcgagacg gccatgtatc gcctgggggg ccggtgtctt ttcatgacgg
    28681 cccaggacac ccagatttcg aggcaggagc ccgcggcgga cacggcccgg gtcctctccc
    28741 ggtacctgga cggcatggtc gtgcgaacct tcgcccagga agagctggaa accctggcaa
    28801 agttttcgac catccccgtc atcaacggtc tgacggacct gtttcatccc tgccagattc
    28861 taagcgacct ggtaaccatc caggaaagac ggggaagtct cgagggcctt cgagtggcct
    28921 ggatcgggga cggcaacaac gtcgcccatt cctggatcaa cgccgcagcc ctgctgggct
    28981 tcaccctctt tcttgcctgt ccccccgggt acgcacccga cccggcggtc ctcgaatggg
    29041 cgcgggcgca gaaccgggga gaaattcacc ggttggaaga tcccaaagag gcggtaaaat
    29101 ccgcagacgt tctgtacacg gacgtgtggg ccagcatggg ccgggaagag gaagcggcgg
    29161 tgcgccggga ggcgttccga ccctaccaga tcaacgaaga actgctggga gcggcgccct
    29221 cccatgccat ggttctccac tgtctcccgg ctcaccgggg agaagaaatc accgacgccg
    29281 taatggaagg tcctcaatcc gtggtcttcg accaggcgga gaatcggctc cacctgcaga
    29341 tggcgctcct cgaatggctt ctgaatcaag ttcgatgatg gaaaggagaa aatccccggc
    29401 atgaaggttc aaaaagtcgt tctggcttac tccggtgggc tggacacgtc catcattctc
    29461 aagtggctga tcgagaccta ccagtgcgag gtagtggcct ttgccgccga cctgggacag
    29521 ggggaggaac tggacggcct cgaagccaag gccctggcca cgggggccgt taaggcccgc
    29581 atcgaggacc tgagggagga attcgtccgg gattttgtct ttcccgcctt tcgggccaac
    29641 gcgatctatg aaggtcaata cctgttgggg acctccatcg cccgccccat cattgccaag
    29701 caccagattc gcatcgcccg ggaagaaggg gccgacgccg tgagtcacgg ggccacgggc
    29761 aagggaaacg accaggtgcg cttcgagttg gcctacctgg ccctcgagcc ggggatcaag
    29821 gtgatcgccc cctggcggga atgggacctg cgatcccgca cggccttgat cgagttcgcc
    29881 gggaaacggg gcatccccat tcccgtaacg gacgagaagc cctacagcag cgaccgcaat
    29941 ctcctgcata tcagctatga gggaggaatc ctcgaggatc cctggctcga gccggacccc
    30001 aagatgttca ccctcaccac ggacccccaa gaagccccgg accagccgga aatcatcgag
    30061 cttcagttcg aacggggaga cgccgtggcc ctcaacggga cccgcctgag ccccgcccgc
    30121 atgcttcatg aactgaacgt cctgggagga aagcacggaa tcggccgggt ggacctggtg
    30181 gaaaaccggt ttgtcgggat gaagtcccgg ggagtctatg aaacgccggg gggcaccctt
    30241 ctgcgggaag gacacagggc cgtggaatcc atcaccatgg accgggaagt catgcaaatc
    30301 cgagactcct tggtgcccca atattccagg ctgatctaca acggcttttg gttttccccc
    30361 gaaatgctgc tgctccagga catgatggat cgggcccagg agaacgtctg gggtaccgcc
    30421 cgcctgaaac tctacaaggg aacgtgccgg gtcgtcggtc gcaaatcaga tcgttccctc
    30481 taccagccca gctttgcgac gttcgaggaa gacgacgtgt accagcaagg ggatgccacg
    30541 ggcttcattc ggctccaggc cttgagactg cgaatcgaat ccctcgtgcg cagcggcctc
    30601 ccccggcact aatccctcaa gggtagagag accaaggagg acccgtttat ggccgaaaag
    30661 ctgtggcaag gccggttcga ccaaccgacc aacaagaggg tcgaagacta cacggcctcc
    30721 atccacttcg acaacaggct ctatcggtac gacatcgagg gcagcgtggc ccactgtcga
    30781 atgctgggcg agtgcaagat catctcccac gacgacgcat ccctcattgt ccaggggctg
    30841 ggggaaatcc tccgggagat cgaacggggg gaactgagct tggatgccgc catggaagac
    30901 attcacatgg ccatcgagca acgcctgatg caaaaaatcg gggaggcggg gggcaagctg
    30961 catacggctc gaagccgaaa cgaccagatt tgcctggaca tgcgcctgta catgagagac
    31021 gtgctccttc aatgccgcag cgccatcctg gacgtgcgga aaaccctcgt gtccctggct
    31081 cagcagaacc tgcacgtcat catgccggga ttcactcacc tccagcacgc ccagcccgtt
    31141 ctcctgtccc accacctcat ggcctactat gaaatgctgg gccgggacga tgagcgtttt
    31201 tccgagtgct tccgacgcac caacgtgctt cccctgggca gtgcggccat ggcgggcaca
    31261 tcccttccca tcgacatgac gtggacagcc aagtacctga actttccccg ggtgaccgcc
    31321 aacagcatcg acgccgtgag cgaccgggac tacctcatcg agttctgcgg cgcttcgagc
    31381 atcctcatga tgcacatcag tcgattggcg gaggaactga tcctctggtc cacggtcgag
    31441 ttcggattca tcgaaatcag tgatgccttc tgcacgggat cgagcatcat gccccagaag
    31501 aagaacccgg acgttcccga actgatgcgg ggaaaaacgg cccgggtctt cgggaacctc
    31561 atggccctgc tttccctcac caagtccctt cccctggcct acaaccggga cctgcaggag
    31621 gacaaggagc cggtcttcga cacggtggat accgttctga gtaccttgcg gctgttggcc
    31681 aagctgcttc ccgaaatccg attcatggac gcgagaatgg ccgaagccgc cgcagcaggc
    31741 tacaccctgg ccacggacct ggcggactac ctggtgcgca agggggtgcc cttccgcagg
    31801 gcccatcacg tggtggggca gattgtgcag tattgcgtgc aggaaaagaa agagctccag
    31861 gattgcagtc tcgaaaccct gaagaatttt cacaaggcct tcgacgccga cgtcttccag
    31921 tccctggatc tcttgggcgc catcgatcag aggatttccc ggggaggtac cgccacgtcc
    31981 cgggtgcagg aagccatcga acaagcgaag aaagacctgg aggtcgagga gacggggttg
    32041 aaaaacccgg cgtgaagcgg acttccccgg gaatctcccg gggagcgcat ccaaaatatt
    32101 gtgaaagatc catgacctgc aactcttagc gggaactttg cccatgattc tgccgaaccc
    32161 ctgcgacacc cgattgcaca acgtcttgtg gggcatccct gtcctgctct tgggattgct
    32221 tctggccggc tgcgggacga aatcctttcc caggtccctg tgtgacgacg ctccgtccgt
    32281 ggtccaggac cttcaggcgc aggtacgatt gaagagggtc gaactccaat ggtccgtgcc
    32341 cggggtggaa cccgggaacg tcaagaagtc cggacatcgg ttctccgtgc tcaagtcgga
    32401 gatcaggtgg gagaaccgaa actgcctgga atgccccgtg cccatgcagc acgagatcca
    32461 gcggatcgag ccgccctatg cgggcttcct gacccccgat tccacgaaga tctcctttgc
    32521 cgacggttcg gtggagatca atcgagccta ccggtaccag gtggcgctgc tggacaagcg
    32581 aggcgcccag gtgactcttt cgaacccggt gatcgtgaag gtgatgcccg cccccggcat
    32641 tccccgggaa ttgctggcgg cgccggaaaa ccagggcatt ctcctgcaat ggaagccccc
    32701 cgtgaaggat gagcacggac gccccatcca gggagaggtc cagttcgcca tcgaacgtca
    32761 tgcacccgac aatccgtggg aaagagtgac ctcgatcccc attcgcggca atcttttcct
    32821 ggatcctgcg gtgacctcca gcgagaccta cgactaccgg gtcattcccc tggtggtgtt
    32881 cgaagggact tcgatcctgg gggaacccgc cagcattctg cacgtgaaag cccccggcgc
    32941 ccttccccct cccccgccca acaccgtttg ggtcattccg ggaaagggtt ccctcgaggt
    33001 ccattggacg gagcacaaga ccaaggtggg gggataccac gtgtaccgtc gggaggggaa
    33061 agaaatcatc cgcttgacgg ccggccccat ccagcacccg ccttacgtgg acaagtccgt
    33121 gaaaaagaac aacgtgtacc actatgcagt ctccgccgtg ggccttcagg cggaccaacg
    33181 ggaggggctg ctttccaagt gggcggagat ccgggcactg atgtttgaat aagcgccctt
    33241 tcatccagag agaaagaacc ggcatgcacc attttcaatt ccggaacggg gaactctact
    33301 gcgaagaagt tcccttaaga gagatcgtcc aacgggtcgg cactccctgc tacgtttaca
    33361 gtcatgccac cctgcaccac cacttctcgg ttttcgacgg cgcgttctcc gaggtggcgc
    33421 acctgacctg cttcgccgtc aaggccaact ccaacctggc cattctgagg ctgtttggaa
    33481 ccctgggggg aggggcggac atcgtgtccg gcggcgaact gttccgggcg ctcaaggccg
    33541 ggattcccgc ccggcgaatc gtgtactcgg gggtgggcaa gaccgccgag gaaatcgact
    33601 acgccctctc ccaggggatt ctgatgttca acatcgagtc catggaagag ctggaagccg
    33661 tcaatgcccg ggcggcggca agaaagtgcc gggccggcat cgccctgcgg gtcaacccgg
    33721 acgtggaccc ccagacccac ccctacatct ccacggggct cagcaagaac aagttcggag
    33781 ttgatatcga aaacgcggtg aaggcctatc agaaggcccg gaccctggag cacgtggaaa
    33841 tttgcgggct gggctgccac atcggaagtc aactcgtccg ggtggagccc tttgtggaca
    33901 ccatgaaacg actgcggatc ctcttggaac ggctggacgg gctgggcatc tccattcgct
    33961 acctggacct gggaggaggg ctgggcatcc cctacgaccg ggaatcgcct cctcatccca
    34021 gcgagtacgc cgaggccatt ctcgaggagc ttcgggggtt gccgtgcacc ctcatcttcg
    34081 agcccggccg ggtgatcgtg ggaaatgcgg ggattcttct cacccgggtg ctctacacca
    34141 aggagacccc cacccggggt ttccttgtcg tggatgcggc catgaacgac ctgatacgcc
    34201 cgagcctgta cggctcgtat caccagatcc agcctttgcg caaagaagac gttcagacgc
    34261 gggtggtgga cgtggtgggc cccatttgcg aatccgggga ttttctggct cgagaccggg
    34321 aactgccggt tgtttcgtcc ggagaatgcc tcgccgtcat gagtgccggg gcctatggat
    34381 tcactatgtc ctccaattac aactcgaggc cccgggccgc cgaagtcctg gtgaaggacc
    34441 gggattggtt cgtcatccgt cggagggaaa cctgggagga tctcgtccgg tcggaatcca
    34501 tccccgactt tctgcaatga cctgccaatt ggagagtgac caggaatgcc ggaagaatcc
    34561 cgaaaaatcc gtttctacaa gatgaccggc agcgggaacg atttcatcct catcgacaac
    34621 cgcacgggcc gcttcgaccc cgaccggtcc cgtagtctcg tgcgggccgc gtgccggagg
    34681 aaattgtccg tcggtgcgga cggtctgatc ttcatcgaaa acgatgggga ggcggatttc
    34741 aagtggcgct tcttcaacgc cgacgggagc gaagccgaaa tgtgcggcaa cggggcccgc
    34801 tgcgccgccc gcttcgccca cttgacgggg attacgaccc ggttgaatct ttccttccga
    34861 accctggcgg gaatcatcca ggcggaaatc aagggagacc gggtcaaggt ccgcatgacc
    34921 tctcctcacg gccttcaaac ggacctgcat ctttcggcaa acgaacgccc attccttctc
    34981 catttcatca acacgggagt cccccacgtg gtgcacctgc tggaaaagga gtcggacctg
    35041 gaggagacgg acgtggaacg gtgggggcgt ctgtttcgat ttcaccccca tttccacccg
    35101 gcgggaacca acgtgaactt cgcccgggtc gaggacgccg gacacatggc cattcgcacc
    35161 tacgagcggg gagtggaagg ggagaccttg gcttgcggaa caggttccat cgcttcggct
    35221 ctcgttgctg cttcgaagga cttggtgcaa tcccctgtgg aagttcgaac acgaagcggc
    35281 gaggtgctga ccgtttactt ccgggggccg acgggacccg gcggcccgga aggcgcatcc
    35341 ttcacggatg tctacctgga aggaagcgcg aaggtggtat acgaggggga gctttgggac
    35401 gaaacgttac agtaacggat tagggaggta gtcatgtttc agggcgcgat ggtggcaatc
    35461 gtcacccctt tcaagaacgg ccgggtggat gaggagtcgc tcaggcagct gatcgacttc
    35521 cagctggcca acgggaccca cggcattgtg ccgtgcggca ccaccgggga atcggcaacc
    35581 ctttcccacg aagaacatga gcgggtcgtg gagatcaccg tcgagcaggt ggccgggaag
    35641 gttcccgtca tcgccggaac gggctccaac agcaccgagg aagccgttcg gctgaccaaa
    35701 cacgccaaga aggcaggcgc cgacgcggcc ttgatgatca gcccctatta caacaagccc
    35761 acccaagaag gcctgtttct ccatttccag aaggtggcgg ccgccgtgga catccccatc
    35821 gttctctaca acattcccgg caggacggcg gtcaacatgg aggcggacac catcgcccgg
    35881 ttggcgaaga tcgacaacat tgtcggcatc aaggaagctt ccgggtcgtt gaaacaggtc
    35941 acggacatca tcgctcgatg tcccggggat ttcaccgtgg tatccggtga ggactacctg
    36001 acctacccca tgctttgcgt gggaggaagg ggcgtcattt ccgtggtgtc caacgtggcg
    36061 cccaaggaca tggcggacct gtgcaacctt catttctcag gcaaacaggc cgaggcccgg
    36121 gagctctact atcgcctctt gcccttatgc cacgccctct tttacgaaac gaacccggcg
    36181 cccgccaagg ccgcactggc catgatggga aaaatcccca gcgaggaagt ccgcttgccg
    36241 ctggcgccca tgtccgaagg aaaccgcaac cggctgcgca aggatctcga agcgtacggg
    36301 ctcctgtaga ttcggcgggg catccttcat ccgtgttttg gccgttccgc ctgcaccccc
    36361 ttcgggggca cgcaacggct tcgagatggt cgagacggca ataagatcgt agcgaggagg
    36421 aaatcatggt ccgggcagcg gtttccggca ttgccgggag gatgggcaca cgcatcgccc
    36481 agcttatcgg agaaaccgac gggattgcct tgacggccgg gttcgagcac gagagccacc
    36541 cggcggtggg agaggacctt tcccgggtcc tgggtgggaa acccacggga cgaatcattg
    36601 cgggcagcat cgaggacgtt ctcgaggaag tggacgtggt catcgatttc accatgcccg
    36661 ccgcttctct ccggcacctg gaaatggcgg cttcccgggg caaggccatg gtcatcggca
    36721 ccaccggctt ctcccgggaa caggtcgccc agatccggca accggcctcg aaaatcccct
    36781 gcgttctggc ccccaatatg agcatgggag tgaacgtgct cttcaagctc gtgggcgatg
    36841 cagcgaaact gctgggttcc gggttcgatg tggaaatcat cgaggcccac catcggttca
    36901 agaaagacgc tcccagcgga accgccgtga aactggccca ggtggcggcg gatgccttgg
    36961 gccgggacct cgaagcctcg ggcgtctacg caaggcacgg gatcatcgga gagaggaccg
    37021 aaacggagat cggaattcag accattcgag ccggggacat tgtgggagag cacaccgtga
    37081 tttttggagg aatgggagaa cgaatcgaga tcacccacaa ggctcacagc cgggacaact
    37141 tcgccagggg ggccatccgg gccgctctct gggtggtgaa ccaacccccg ggactctacg
    37201 atatgcaaca cgttctcggc atccgataac gccaagtgcc gggagagtgc cccgaacggg
    37261 gttcttcttt cccgcatcgt ccacggcccc ggcccatata cgggatcggc gaaggggaaa
    37321 cccgcaactc acgctcctat ccgatttcgc tccaccggag gttttcatga agatcatcaa
    37381 ggcagaacga ctgagacgac ttccacctta cctgttccag gaaatcgacc gactcaaggc
    37441 cgaattggtt gccaagggag tggacgtgat caacctgggc gtcggggacc cggatcttcc
    37501 gacccccgat cacatcatcc ggcgactgca aacagccgcc gaagatcccg aaacccacca
    37561 gtatccctcg tactcgggca tgaacgattt tcgtgtcagt gtggccggct ggtaccggcg
    37621 gcggttcggg gtcgaactgg accccctccg cgaggttctg actctcatcg gatccaaaga
    37681 aggtcttgcc catctgccat tggcgtgcat caaccaggga gacctggccc tggtcccttc
    37741 ccctgcctac ccggtctacc acgtcgccac cctgtttgcc gggggagaat ctttttttat
    37801 gcccctgcag cgtgagaacc ggtttctacc cgatctgaag gcgattccgc cggacgtggc
    37861 ccgccgggcc cggctgatgt tcctcaacta tcccaacaac cccacgggag ctacggcgga
    37921 gcgcgatttc tacgaacggg tggtggaatt cgcccgggaa tacgagatcc tcgtctgcca
    37981 cgacgcggcc tatacggaaa tggccttcga cggctaccgg cccatgagct ttctcgagat
    38041 tcccggcgcc cgggaagtgg ggatcgagtt tcattccttg tccaagacct acaacatgac
    38101 cggatggcgc ctggggtttg ccgtaggcaa cgcgcagatc ctggagggtc tggggcaggt
    38161 aaagagcaac atcgattcgg gggccttcaa cgcgattcag cacgccggga ttgccgccct
    38221 cgagggaagc caggaatgcg tgggccggat gcggcaaatc taccaggagc ggcgggacgt
    38281 cctcattgcc ggactgaaga aggtcggcct cgaccccgac ccccccaagg ccactttcta
    38341 tgtctggtgt cccaccccaa aagggtacaa ctccaaggat ttcacctccc tgctgctcag
    38401 agaagccggg attgtcacca cgccgggcag cgggtttgga gaacccgggg aagggtacgt
    38461 gcgcatggcc ctgaccgtca ccaaggagcg gatcgaagag gcggtcgggc gcattcggaa
    38521 gctgagcttt tgactccggg ctgtccggct ctcattttgt gggagacacg ggggcggaaa
    38581 tccgctaaac atggacccca tggaacaagt attcattggg attggcagca accggggaga
    38641 tcccgcaggg atttgtcgag acgccgttgg ggccctggac cgcacgggat ccgttcgagt
    38701 cctgaggatg tcttccctgt atcgatccaa gccggtgggg gtccttgacc aggactggtt
    38761 cgtaaacggg gtggcctcct gcgagacgga tctcgaaccc ctggaattgc tggatcgggt
    38821 cctcgagatc gaaaaggcgt ttgggaggcg aaggctggta cgttggggac cgcgaacgtt
    38881 ggaccttgac ctcttatgtt acggccggag gcgggtcgat cattggcgcc tcaccatccc
    38941 tcacccgaga cttcaccgga ggctgtttgt gctcgtgccc ctggcggaga tcgccccgga
    39001 ctgggtgcac cccgtccggc agctcacggc acgacagttg atcgatcaat tgatgaaaca
    39061 agatcacgac caggtggttg tgccgcttgc gggatagaca tggcacggat ccttctcatt
    39121 ctcttgctcc ttttcatcgg gtaccgaatc ttcaagaggt tttcctcgcc cggaaagcag
    39181 gtcccgttcg gttgggggga ccggcgggag ggaaccggag acgcggaact cgtccaggat
    39241 ccccagtgcg gcacctactt ccttcccggc agcggcgtgt ccgcaagaat cgacgggcgg
    39301 aagctcacct tttgcagcga acaatgtcgg gacgattatc tcaagaaccg ttcgccatcg
    39361 aagggtccct aaccattgaa ggaggctgtc gtgaaatttt ttatcgacac cgcgaacttg
    39421 gacgaaatcc gggaagctca cagcatcggg atcctggacg gagtcacgac caatccttca
    39481 ctcatcgcca aggagggcat cgccgggcgg gaggctttcg aggagcacat caagaccatc
    39541 tgcgagctgg ttcaagcccc ggtgagcgcc gaggccttga gcatggtctc gggggacatg
    39601 atcccggagg ctcgctccct ggcgaagatc cacgaccacg tggtggtgaa gattcccatg
    39661 accaccgagg ggctcaaggc cgtgaaacag ctctcggaag agggaatcaa aaccaacgtc
    39721 accctggtgt tttccccctt gcaggccctc atggcggcaa aggccggggc aacctacgtg
    39781 agcccctttg tgggccgcct ggacgacatc gcccacgacg ggatggagct cgtcgaacag
    39841 atcgtggaaa tcttttccaa ctacctgttc gaaacggaaa tcatcgtggc cagcgtgcga
    39901 aacccgctcc acgtgctcac cgcggcgaag ctgggggcgg atatcgccac cattcccttc
    39961 aaggtcatcg aacaactggc ccgccatccc ctgacggaca tcgggatttc caagtttttg
    40021 gaggattggc aaaaagtgca gaagagagta taggataggc tgtcttcgac ccagagatgg
    40081 gaacgcagtg gacaaaacct tgaaaggatg gaccatgaga agcagagtag gtcgggtctg
    40141 ggcgttgccg ggtgctgccc tgcttttctg cggcatcatg atggggacgc cttcttcccg
    40201 ggcggaaatt taccgctacg tggaccggga cggcgtgatg cacttcacca acgtacccac
    40261 catgccgcaa tatcgaagag tccccaacct gccccagatt gccaccctgc gcccgaatta
    40321 ctatcggctc ctgcccaaca ggacgtccgc atcctcctgc gaaagtctaa accagtatgc
    40381 ttacgatccc cacatccgcc tggtctgcgg acggcacggt ttggacgatc ggctggtgaa
    40441 ggcggtcatc aaggccgagt ccgcctttga ccccaatgcc gtatccccca aaggagccat
    40501 ggggcttatg cagctgatgc cggacacctc ccgggacctg ggagtcgtca atccttttga
    40561 tccctacgag aacatcgacg ggggcgtgcg gtacctcaag gccctgctcc ggcgattcaa
    40621 caacgatgtt cggctggcac ttgcagccta taacgccggc cccgaaagcg ttcaaaaaca
    40681 tgggggaatt cctccctatg acgaaactca gatctatgtc cagcgggtcc tggaatacta
    40741 tggtcgctac ggcaaatagg cgttgccgca aaggcggggt ccatctcccg ccaagaccga
    40801 tacgacgccg gttggggccg ccctgaaccc gaaagctgca tggctgcaat gacacgattt
    40861 gacttttcga gggaaaccct caccaactgg cccaatgtcc tgacctacct caggatggcc
    40921 tccattcccc tcatcatcct ggtcctcatg cctccgccca cgggaagcgc tcacaatacg
    40981 gccttttttc tctttgccgt ggcttccctg acggactacc tggacggaat cctggcccgg
    41041 cggcacaacc aggtcacatc cgtgggaaag ctcctggacc ccctcgccga caagcttctc
    41101 acctcggcgg tcctcatcat gctcatcccc ctgggaaaag tccccgcctg gttggtcttt
    41161 ctgatcatcg gtcgggagat cagcatcacc ggcctgcgct ccatcgccgc aagccagggc
    41221 ctgatcctca acgccagccg catgggcaag aacaagatga tctcccagac catcgggctt
    41281 ctctttttgc tcctctacgt gcccggcatc caagacgtac tggacggcac gggcctcttc
    41341 ttcctctgga tttctctcat cctgggttat tggtccgcca tggactactt ctcgggcttc
    41401 tatcgggaag ccacgagaca ctggcggagt tgacccgaca cagggaggat ttccgtggcc
    41461 ttcaccctcc ccccgtacgt tccgcccgat ttccgtcagg ccgccctgga ggaagcaccc
    41521 accgtacgtt tcgccccggc cccccgggat ggggtcgctc ccgaagggta ccacgccacc
    41581 tccatctatc ccgaatacct gaagctgcag ccgggaggat ggcatctgct ccgagaatcc
    41641 cgcatggact gcgtggtggt gctcggacag gacgcaaccc cctccgtgcg tgaatttcga
    41701 cacctgaagc aaggcgaccc ggtggcctgc ggacgggaag aaaacggaga ggaagggatc
    41761 ctggttcata ccacgggctt ttccgccccc ggggatcttc cggaaaagtt tgcctttcgg
    41821 acccaggtga cccgagagac ttccttttcc ttcgactacg acgccctcta cgatctgctc
    41881 gaatatgaac gggaccacgg tttcatcttg tgggtgctgg ggcctgccgt ggccttcgac
    41941 cgggactccc gccgtgcctt ggttcaaatc atcgcaggcg gccacgttca tggcctgctg
    42001 gcaggaaacg ccctggccgt ccacgacatc gaagcggccc ttttcggcac cgctctgggc
    42061 caagaaatct attccaagcg ccaggttccc aaggggcact acaagcacct ggatgccatc
    42121 aataccatcc gctccctggg ctccatggaa gaggccgtga cacgcaaccg gattcccgac
    42181 ggaatcatgc ggactctcat cgacaggaag attcccaccg tcctggcggg atccattcgg
    42241 gacgacggtc ctcttcccga ggtcatcgcc gatgtgtacc gggcccagga tcaaatgagg
    42301 accctggcgc gacgggccac caccgtcgtg gccatggcca cccagctcca cgccatcgcc
    42361 gtcggcaaca tgcttccttc ctatcatgta accccggaag gacgggtccg gcccgtctat
    42421 ttctacagcg tggacatgtc cgaatttgcc gtcaacaagc tggccaaccg agggtccctg
    42481 acagctcact ccatcctgac caacgtccag gacttcatcg tgatgctcga gcgaggattg
    42541 aggaaacgaa actcctaggc cgagtccccc cgggtcagtt gggtcccggc cttccccgcc
    42601 acggcatcct cgatgtgtcg aatggacgtg atcacggccc ttttccctcc cgagtccaga
    42661 aattgtatcg ccgcttccac cttgggtccc atggagcccg ggggaaactc ccccgcctgc
    42721 aggtatttgc gggcttcttc cgggttcagc cgggacagga accgctggcg cggggtcccg
    42781 taccacaggg ccgctccctc cacgtcggtg gcgatgacga agacatccac cccgatttct
    42841 tcacagagct tggcgctggc caggtccttg tcgatgaccg catccacccc gtggaaggtc
    42901 ctgctttcct tgaccaccgg gatgcctccg ccgccgcagg aaatcacgat gaatcccatg
    42961 tccaccagca tccgaatctc ccgtttctcg acgatggtca cgggttttgg ggaagcgacc
    43021 actctccggt aacccgccgg ggtccgtacc gttgcatacg gcagcccaag ggcctcctcc
    43081 cgggaaaaaa ccggcccgat gggtttcgaa gggttttgaa atgcggggtc cttttcgtcc
    43141 accaccacgt agctgagaag agtcacgaag aaccgcttgc cgtccagtcc cagcttcatg
    43201 agctcattat ccagggtgga ttcgatcatg tagccgatct gcccctgggt ttgagccacc
    43261 aggatctcga ggggaagccg agggacctgg gaacagcatt cctgctggag cagcaggttc
    43321 cccacctggg gtccgttgcc gtgggtgatg acgatgcggt gagtccggga aagccgggca
    43381 atttgccgaa tggggaccac taggttttcg aactgttccc gaatggttcc ttcctggccc
    43441 tttcggatca gggcgtttcc ccccagggcc accagcaggg tctcgggttt gccgggaacc
    43501 ggcttgctct tgggcatgtt tcctcacact cctccggatt gcgccccatg ggacgtggaa
    43561 cgcggatggc ggttgaaata ccctctattc tatactaccg tgactccccc cggtagagcg
    43621 ttgcaacaac cccgggaccc ctcgacgatt ggaccggtgc ccaagcatgc ctcaagatcg
    43681 ccaatggaag aaagtccttt cctgggccct ttacgactgg gccaattccg cctttgccac
    43741 cacggtcatg gcggggttct tcccggtttt cttcaaagag tactggagcg ccggggccgg
    43801 cgttaccgag agcacctttc agctgggaat cgccaattcc ggcgccagtg tcgtcgtggc
    43861 ctgcatggcc cctctcctgg gagccgtggc cgactgcggt cgcgccaaga aacggttcct
    43921 cttcttcttt gccctgctgg gaatcctcat gacgggttcc ctttacttgg tggccaaagg
    43981 agaatggaag atcgcggtcg tctgctactc cttgggcacc atcggctttt ccggggcaaa
    44041 catcttctat gattccctca tcgtgggggt cgcccggggc aggaggctcg atttcgtctc
    44101 ctccctggga ttcgccctgg gatacctggg cgggggcctg ctgttcgccc tcaacgtctt
    44161 catgaccctt tacccccgat ccttcggcct cgaagacgcg gcccaggccg ttcgaatctc
    44221 cttcctaagc gtttccctgt ggtgggcggt cttctccgtt cccatttttc tttgggtgga
    44281 agaacccctg ggcacaggca acaaaacggc ggggggatgg gccgccgtgc aggggggaat
    44341 gcggcaaatc gcctccacct ttcgcgaaat ccggcagctg cgaaccgtct ttctcttcct
    44401 gatcggctat tggctttaca tcgacggcgt ggacaccgtc attcgcatgg cggtggacta
    44461 cggattgtcc ctggggtttt cctccaagag ccttctcacc gccctgctga tcacccagtt
    44521 cgtgggcttt cccgccgcca tcctgttcgg gtggctgggt gagagactgg gggccaagac
    44581 cggaatattc ctggggatcg ccgtgtacct gggggcaacc ctttgggctt atttcatgga
    44641 ccgggagatc gagttttacg ccctggccgt ggtcatcggc ctggtccagg gaggagtgca
    44701 gtccttgagc cgctccctgt acgcccggat catccccccg gggaaatcgg ctgaattctt
    44761 tgggtttttc aacatgttgg gaaagtttgc cgccgtcatc ggacccgccc tcatgggctg
    44821 ggtgagtctt ctcacgggca atccacggtt ctccatcgtt gccatcgccc tgttgtttct
    44881 ttcgggcgcc gccattctgt ccttcgtaca cgaagggccc caagggacag cgctttcgtc
    44941 ctgacgactt cccggacccg gcgccggggc acggcccccc ttccggggag gggagacgac
    45001 ggagcatgcc ggcggtgatg gggcgaaaac gggggcggga atctttgcgg aaggcccggg
    45061 gacccgggag cccgatcccg gcctctacag ggaggtcagc ccttctcgaa tggcgtactt
    45121 gaccaggtct gcgacggatt gaacgtttag cttgttcatg atctgctggc ggtgggtctc
    45181 cacggtcttc atgctgacgt ggagcgaatt ggcgatctgc ttggtgctgt ttccttcggc
    45241 caataactgc agcacttccc tctcccgggc ggtcagcacg gaaaagccgg acccgtcggt
    45301 ggcggccagg tgtcgaatgt agtcctcgac cacggtcccg gccacctggg ggctcaggta
    45361 ggtccgcccg gccaggacta cttgaaccgc ctgcgccagt tcttcgaatg cacaatcctt
    45421 gagcaggtag gcggaggctc ccgccttgaa catctgggcc acgaaacgcc tgtcggagtg
    45481 catggaaagg gccaccacgc gaatccgggg attttccgcc acaatctgcc gcgtggcttc
    45541 aatcccgttc aagcccggca tgccgatatc gacgatcacc aggtcgggaa ggagctcccg
    45601 tgcaagtcgg accgttgtcc gaccattgtc ggcctcggca acgacatcca tccccggctg
    45661 cttttccagc aaggaacgca aaccgtcccg gacaatcttg tgatcgtcgg ccagcaggat
    45721 acgacagccc atcgcttgca cctccctcct catgcccccg caacacccga accgcgagga
    45781 gcctcgggaa ggccttcggc ccgggtaggt ctgccggggg ccgtcggcat gaagaaacaa
    45841 gcgggtcctg cggcctccac cggaacccct gctttcccgc tccgggttgg cccgaaaagg
    45901 ccctgcgggg gtcccccggc aagaagatcc catggagaag agggagctcc tatttcttct
    45961 tggagggttt tgcttcctcg attttggtca cttccaggac tcttcctttc tcccccttgg
    46021 tgtagatgcc ccgcaccttg accttctttc ccaggaactt ggaaaagtcc tgccccccga
    46081 ggttgtactt ggtcttcccg gtgatgagaa catatcccgt gtccgacttg tagatggtcc
    46141 cgagcagggt ggccgttttg gacttggatc ctcgagcatg cgcgtctccg gcatccaggg
    46201 ccgccgccag gcaggcggac atgaacagca tcgccagtac caccgcaatc catcgacgtc
    46261 tttccatacc gactccttca gatttcagac cacgatccca ccacgcatcc cgcactcggt
    46321 cccaacatgg accggaaagt tgcaaacacc cactttccta taccgggcaa ggaaaccggg
    46381 acggtatcgg gaaaatcctg atttttgagt tccatatggc tgattttccc aagcagcgca
    46441 tcgtcgaacc cttcagggtt tgaggggagc cttgagggtc acgcacgttc cctccccttt
    46501 ccgggattcg attcggaggg tgccgttcac aaaaccgagt ctctcacgaa tgctgaacaa
    46561 tccgaacccg tccgcccgtc cccatccctt tcctcccgcg gggacctcga agcccacccc
    46621 gtcgtcgatg acgctcacca ggatgaaacc ctcttctctt cgagcaacga tcctgaggtt
    46681 cttggcatga gcgtgtttgg ccacattgac caccagttcg ttggcggcct gaaacagcag
    46741 gccccgaatg tcgtcgtcca ggggtttcgg ttgtttgtcg tcttcgaacc gggcgtccag
    46801 tccgtgctgt ttttgggcct gttccgtcag ccattccagg gctgcttcga agcccagctc
    46861 gtagaggatg ggggaactga tcctgaagag gaggcttcga gtgtcctgaa tcgcctgttc
    46921 gatgaggttg cggacctgct cgacggcggc tttctgctcc ggggatttcg aggtttcccg
    46981 gagggcgccc aggtggatct tcgacatggc cagggcctgc cccacccggt cgtggagttc
    47041 cgtggcgatc ctgcgccgct cccgttcctc cgccaacgaa agctgggaag ccagggtcct
    47101 catttccagg gctctctgct cgagggattc ggccatttcg tcaaaggcac g
//
LOCUS       DAOOVC010000015        46987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_492_length_46987_cov_6.059235, whole genome shotgun sequence.
ACCESSION   DAOOVC010000015 DAOOVC010000000
VERSION     DAOOVC010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 46987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46987
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_492_length_46987_cov_6.059235"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(344..3142)
                     /locus_tag="PLM79_03645"
     CDS             complement(344..3142)
                     /locus_tag="PLM79_03645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85429.1"
                     /translation="MAKQALKSIPDVSEDPMTDILPGSAPWDGDSQTRSADSGGPAVP
                     SNGTPRLEDPLPHFSANSQALPAAFLKSSHDERNVLRPVFGRNQQTTGVARFYLHPPK
                     SIPVVEDVRPRVQGKFLYVGEDKFYICGVTYGPFRPEEDGSEYHTPEQVERDFTQMAS
                     MGLNSVRTYTVPPRWLLDAAQRHGLRVMVGLPWEEHVAFLDDKKLANDIERRVREGVR
                     QCAGHSALLCFTVGNEIPASIVRWYGRRKIEAFLERLYRAVKEEDPGALVTYVNFPTT
                     EYLDLPFVDFVSFNVYLETREKLEGYLARLQNLAGNRPLVMAEVGLDSIRNGEDKQGA
                     TLSWQIRSTFAAGCAGIFVFSWTDEWFRGGHEILDWGFGITDRERNPKTSLSAVRKAF
                     SEVPFPPDLPWPGISVVVCTYNGKRTIRDTLEGLKKVEYPNFEVIVVIDGSTDGTEEV
                     VREYGFRMIKTENRGLSSARNTGMRAAKGEIVAYIDDDARPDPHWLTYLAATFLKSDF
                     VGVGGPNLAPPTDGPIADCVANAPGGPIHVLLTDREAEHIPGCNMSFRKWALEAIDGF
                     DTRFRIAGDDVDVCWRLQQQGWKLGFSPAAVVWHHRRNSVKAYWKQQLNYGKAEADLE
                     KKWPEKYNAAGHVPWAGRLYLRGVEQLLGWCRGRIYQGTWGTALFQSIYQPAPDLLWS
                     LPMMPEWYVVVAALASLSLLGLEWAPLLAAFPVFVVAAAVPFVHACWSALRVSFPEGS
                     RAGKSRLKLRALTAFLHMIQPLARLMGRLRAGLTPWRRCAVPGVALPLPWQRSFTVWS
                     EQWRSPLEWLESLEGSLKKLRTVVLRGGDYDHWDLELRGGLLGRVRILMAVEEHGAGK
                     QLIRFRAWPRCAPAGIVIAFGFALLAGTAALSGAWVACAVLNFAAFSIMMLILKECSA
                     AMASVIRILSPLRPRKKG"
     gene            3832..4209
                     /locus_tag="PLM79_03650"
     CDS             3832..4209
                     /locus_tag="PLM79_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85430.1"
                     /translation="MPPGPFPERGSLSPKIGLPVVPRPEPSGQGVGAVYEYLRIDGRV
                     DAEDLILDEIDGRLTLDGGLRGRVQHQVLLQVLTRVFGYASVVDRLEVGERDAEEEQR
                     PISGILGERSIEGSALEGRSGFD"
     gene            complement(4214..5560)
                     /locus_tag="PLM79_03655"
     CDS             complement(4214..5560)
                     /locus_tag="PLM79_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOV85431.1"
                     /translation="MFSCVLRGWMFGVVLLVGTALVCPAGVCEGASEDGVPDPASERP
                     REALSSPTTRVSKQPAEKEKQEEAEGSREVRSTLEEVKEELKEKEYGLDIHGAVVSFY
                     QGAVGERIGKRKITEDAEPGVVGDLKLSYRPRSTFFKDGQFFLRFHAGTGKGSDERLG
                     SKLFANLNTIADDSGPYHHDDGFDRILLEGYYAHEFCNEKLILAVGKTEPLVFIDTNA
                     FANDQYSQFVGKPFVNNPILSNEDEYGPIFAASFTPLEGVTLTALVQSSTHPYALPSG
                     QKDMYHNLLEDPFVAAQVAYSPKIFGQEGNYRLYYWNATYDHPRIGSRGSVQGWGLGL
                     SLDQKITDAVGLFGRFGYHNEDAYEVPWFWSAGVNWKGILPLRAEDQLGIGLAGLKAN
                     EDLRHTGTEVHAELYYRIHLTKYFAVTPDFQWVVDPRGNSGNDPIFAGMVRGEFLF"
     gene            complement(5843..6367)
                     /locus_tag="PLM79_03660"
     CDS             complement(5843..6367)
                     /locus_tag="PLM79_03660"
                     /inference="COORDINATES: protein motif:HMM:NF039354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOV85432.1"
                     /translation="MELEVLRTMKRDQLLDYLEFLLWHYRVMDAFWFLYVEESHGLAQ
                     AEHLNQRVWGKVAGMAAKDLRKRFRIEEKGLAGFLKALRLFPWTLIVGYQIRENPGEV
                     ILTVPHCPPQEARIKQGLGEYACKDMHRDEFTRFAHEIDPAIRVECVYAPPDPRPPEF
                     FCKWRFTMDSSGKK"
     gene            6586..7326
                     /locus_tag="PLM79_03665"
     CDS             6586..7326
                     /locus_tag="PLM79_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85433.1"
                     /translation="MKQWNELTTAGGTGVHPAGPIIAARGLTRVYPLEACEVIGINEV
                     DLEVRAGELVVLKGDSGSGKSTLLALLGGMDRPTRGCLMVAGQDLSTAGVWDLTRFRR
                     EVVGMVFQSFNLLPTLSALENVCLPALLAGVSYGKVRSKAMELLAWLGLESRVHHLPG
                     QLSGGEMQRTAIARALINDPPLILADEPTGDLDSANGHRVMEHLGELNRRQGRTVLIA
                     THSTLADPYATRTVRLKDGGIAEMTWAD"
     gene            7314..9920
                     /locus_tag="PLM79_03670"
     CDS             7314..9920
                     /locus_tag="PLM79_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV85434.1"
                     /translation="MGRLTLFLRLWLWFSFRHLRAHYRRTLAVVAGIALGAAVFTSVR
                     LAVHASIDSFTRTVDTLTGKADRVVIHPGGRVPESVVSQLAKHPWVESASPVMTTYVR
                     AVERWTGPFLLVGIDPILDRPLRTWETRDRDVASGLRASAWIDLLREPFSVLLGDGLA
                     RRLDVREGGRVEIQGPRGNTPFRVVGILESRGLGAAEGGETALVDIATFQEFTGQPGF
                     VDRVDLLFKPGIPSQEVESLRALLPAALVLEMPGESRESGRLLIRSYELNLSVLSFVS
                     LFVGMFLIYSLVALNAASRRHELAVLRCLGASSRLLFVLFLFEGVFFGVVGWLTAVPL
                     SGFLVGRMLHGVSETISLLFVRVRVDQVGLDSREVALSLLVTVLISILAAWQPARQAM
                     KVPPREALLIRDSLAERRGSARKIAFLGTVLILLVWPLSQLPGIAGIPVGGYLGTFLL
                     FAGFSLVSPWVLSLLGDKMPEVLRKVGGEPGFLGARTIRDAGARIAISVGALITATAL
                     FVALVIMVHSFRQTVTAWVNQTITGDLFLRPRMAEFNHYRDPLPRAAVKTLKELETPV
                     QVLPYRRIFLHQGNIRYQFEAIDFPVFLRYAKFLLLEGKPEEMIPKLTEGEGVLISEV
                     FANRSRLRLGDTYRVRIRGVEIHRPVVGIFRDYRTQGGVVHYSLSHFQELTGDDSWGG
                     ARVHLVDPGPDPQASAARVREEIHRRCVECVPGVEITLGNDLRRGVLRIFDETFAITT
                     VLLFIALFIAALGIATTLMVLVLERAREFHILLAVGAERSQVRRMILWEAVLMVLTGE
                     IIGLGCGFCLSWILVYVINRQSFGWTFVYAVDWSSLLASLPLVLAAALLATLPASRWV
                     FRRSPAQILRQG"
     gene            9944..11092
                     /locus_tag="PLM79_03675"
     CDS             9944..11092
                     /locus_tag="PLM79_03675"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOV85435.1"
                     /translation="MRMRRFCGYSTTVLLAVCVVLGGLAPAGGQEGEYLPVTGPCGLQ
                     FPRDHGSHSGYRTEWWYYTGNLETPSGRSFGFQLTFFRRQISPPGFEKHWPEPPSAWR
                     TQNIFLAHAALSDLERGKFYQEEKVARGAVGLSGVTRDSGETRVFVEDWSAGIRESGH
                     ILRAATGDFSLELALVPQKAPVLHGDAGYSRKGGGKERASCYYSFTRLKAEGTLKLGE
                     GTILPVHGEAWMDHEFASSPLEPGIVGWDWFSLQFTNQTELMVYLLRKADGGFLPQSG
                     GTFVTRSGEVVPIAWEDLRVRVVKEWKSPRSGARYPAGWVLHIGSQDLEVVVTPTLED
                     QEMHTPLSTRVTYWEGSVGVAGTMAGAPVKGKGYVELTGYDRPLDALH"
     gene            complement(11266..12444)
                     /locus_tag="PLM79_03680"
     CDS             complement(11266..12444)
                     /locus_tag="PLM79_03680"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV85436.1"
                     /translation="MSNKVDPNLMKDLTAFGLKDAAKCYHCGNCTATCPLSTAENTFP
                     RRFMRYVQLGQKDKILHAPEPWLCYYCGDCSAKCPRGADPGETMMVMRRYLTSLYDWT
                     GFARKFYTSEKFEIVAITIVAILVGVGLLLFNTGSPNWEHAHINSVWPSDKIEIADLV
                     MAAILSFFLITNILRCAKFVMGDLLTKVPLGIYAREAKELLIHVLTQKKFGQCTDRMQ
                     WFVHLMIMTGYATVFLMVVVLVNGLTVETLKFQRDLPEYPLWHPIRLLGYYATFAIMY
                     GTTYAIIGRLKKSKPVYKNSHPTDWMFLILLQLTTLTGIFIHFTRLLDWPATTYFLYI
                     VHMMVAIPMLVLEVPFAKWAHLAYRPIVLYLLKVKEAYFKEQAPAAEPATAAVAVNPE
                     "
     gene            complement(12697..14910)
                     /locus_tag="PLM79_03685"
     CDS             complement(12697..14910)
                     /locus_tag="PLM79_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85437.1"
                     /translation="MEQKIAVYICTGCGIGDALDIEALGKVATKECKAPICRNHPNLC
                     SREGVDLIKGDAAQEGANTLIIAACSPRVMYDVFNFEGCIVERVNLREQVVWCQKPQD
                     EDTQMMAEDYLRMAGAKVKKTELPQPYKPEQEMSRDILVVGGGLAGLTAALEASRAGY
                     SVVLVEKEAQLGGFQKNVSLKVTTPYKEVRSNDLEELIRTVSHEKSITVYTGAKVEKI
                     VGGPCVYEVTIQQNGTSVQHSIGAIVLAAGWKPYDPEKLDKKLGFGASPNVVTNVMFE
                     DLAKGGTLVRPSDGAPLKNVAFLQCAGQRDPEHLSYCSSICCLTALKQAVQIKSRDPD
                     ANVYVVYKEMRTPGQAEDFYRKAQEEGVIFIRSQDPEVRAGVGGLSVEAHDELLGEKV
                     LLENLDMVVLATGMVPVTAFGEDFKAAAKKEGEEEIQVPADAIRVSDVLNLEYRQGPE
                     LPGLKYGFPDSHFICFPYESRRTGIYAAGCVRRPMGTASTKEDAAGAAMKAIQCIELT
                     MQGAAVHPRAGDMTYPEFFMQRCTQCKRCTEECPFGAINEDEKANPLPQPTRCRRCGV
                     CMGACPERIISFKNYSVGMIGDMIKSIEVPEEDEEKPRILVLACENDAYPALDMAGLR
                     RMTYNAWVRVIPMRCLGSMNLVFIADSLSKGIDGILLLGCRHGENYQCHFVKGSELAE
                     IRLSKVKETLDRLVLESDRVRFETVSITDYDRLPALLDSFAERLVEVGPNPYKGY"
     gene            complement(14916..16178)
                     /locus_tag="PLM79_03690"
     CDS             complement(14916..16178)
                     /locus_tag="PLM79_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV85438.1"
                     /translation="MSNTVTQSILVVGGGMSGLTAAVEAAEAGREVYLVEKNPYLGGR
                     VAQLHQYFPKLCPPYCGLEINFRRIKNNPRVRVITLAEVEKISGSEGNFDVTVRMNPR
                     YVNEKCTACDLCAQACSLEIDNPFNYGMDKIKAAYLPHDMAFPLRYVLDPSLVKSPEA
                     QKVKEACPYGAIELDMQSKVLDLKVGSIVWATGWKPYDMTKLDAYGSGTYANVISNVM
                     MERLAAYNGPTQGKIVRPSDGKAPETIAFVQCAGSRDENHLPFCSTICCLASMKQTTY
                     VREQYPEAKMYIFFIDLRATDRLEDFVRSVKEDPNVRFFKGKVAKIEEDPATKNLTLR
                     VEETTSGSLQEITVDMVVLATGMQPNTAESPVPGAVSYDDYGFVASIEAGRGIYGAGC
                     TRTPAGVSESVQDGTAASLKAIQSIVRR"
     gene            16549..16749
                     /locus_tag="PLM79_03695"
     CDS             16549..16749
                     /locus_tag="PLM79_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85439.1"
                     /translation="MEEKQDLRSQWFSYGIKLALSFAFLFPVYFLLYVTFPGSLVALY
                     FISAVLFAVFAPWDTLKQKFLS"
     gene            complement(16862..18067)
                     /locus_tag="PLM79_03700"
     CDS             complement(16862..18067)
                     /locus_tag="PLM79_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV85440.1"
                     /translation="MNASLQALVVEDHVAMRSVVRKMLQQMRYFQLIDDASDGEEAWA
                     KLANGNFDLAVSDVNMPRMDGIKLLKRCRTHIELKGLPFLMISGESLPEWVASAGEWG
                     AYDYIIKPFSYMDLKSRVDGILEKLKDPEELLFREMERRKARGHARSVLERIDQLEHS
                     VASLKLKWLNLKGECLMELGDMESAAVCFEKVMRVSDVFLAAYKNYATVHQNLGNIDK
                     AVEALEKVNCISPMDPDRKVTLGRLLLQAGRTDRGRSVLEGVLRTAAPEEKQGYRLSV
                     AEVYLESGLYAEAEKLYLRALRSNPDSVEAYNRLGIALRRQGKFQEAERYYRLALTTR
                     PNNPAIHYNLGVLHLNRQDVAEAVRSLKKALELDPKFEKAEELLRATLRKQKETAGQD
                     LLCSPGRPT"
     gene            complement(18207..19796)
                     /gene="rmuC"
                     /locus_tag="PLM79_03705"
     CDS             complement(18207..19796)
                     /gene="rmuC"
                     /locus_tag="PLM79_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOV85441.1"
                     /translation="MNGWAFLPEPALFFSAGIVAGGVLAWIFLRWRWMSVLSGQETRI
                     EIERSALTERMAAREEALRAAKEALDQQAIRERRLREELQSALQRQAAAEERGFRVEP
                     LESLLKEKEEFLGRVRSENSELRSRISELETRLHLERTSSQEKLGLIREAQTHLSDAF
                     RALSAEALKSNNQSFLELAKSTFERFHQGARNDLETRQKAIQELIKPIESSLDKVEGQ
                     IKEVERSRTEAYVSLHEQVRALAQVQSRLQKETAQLVQALRTPTVRGQWGEIQLRRVV
                     EMAGMVEYCDFVRQESVAGEQGRLRPDLIVRLPNRRNIVVDSKAPLKAYLEALQAPDE
                     QTRNQKLREHARHIRTHLHQLGAKAYWEQFAPAPEFAVLFLPGETFFSAALEQDPSLI
                     EAGVERNVILATPTTLIALLRAVAYGWRQEQMARNAQAISELGKTLYDRIRSWMAHFA
                     DLRKGLDRAVEAYNRAVGAAESRVLVTARKFKELGAFPGEDPEFPETIDRQARPSSAA
                     EDPGAPTLPPAAPDPRHPPEK"
     gene            20022..20456
                     /locus_tag="PLM79_03710"
     CDS             20022..20456
                     /locus_tag="PLM79_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOV85442.1"
                     /translation="MTECRIPDQPVTDQAVADILKRSRTIAVVGISHKEERDSHRVAK
                     YLKEHGYRVIPVNPKYKEVLGEKCYPDLPSVPEHIDVVDIFRNVEAIPGIVDEAIRIK
                     AGTVWMQLGLAHHEAAQKARCAGVGVVMNKCMKIEHSRLLTS"
     gene            20551..21132
                     /locus_tag="PLM79_03715"
     CDS             20551..21132
                     /locus_tag="PLM79_03715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV85443.1"
                     /translation="MSPRTPFPPAVETRPPLGSIGSVIRYYRTLHGTWDDEAGGPYRL
                     TSLGTWAASRVPHVYHFFKKIRLDRFPLFLDLGSGDGLVTCVAGLFTRAVGIEVDPVL
                     CRTGLQAARALGLTGRVDFACADYRSLQIRRADVLYIYPDKPILPFRELLEDWNGTLL
                     VYGPHFPPEGFEVVEDLRCARERLRVYRNSRTR"
     gene            complement(21260..24196)
                     /locus_tag="PLM79_03720"
     CDS             complement(21260..24196)
                     /locus_tag="PLM79_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOV85444.1"
                     /translation="MTDEKSKKSPQNHEKEGEVSLEEFLRKKAEIDQAIREKFQRTLC
                     IMFTDVVASTEFFELHGDIEGRFMVQRYNDLLRPVIQRHHGRIVKTLGDGLMVAFVKP
                     ADSARAAIEMQKALAVENEGKRPANQIWIKISIHYGPGIVETHDIYGDVVNTSARICS
                     AAQSGDILVSKDVIDALGGEPGIAHDYAGAKSFKGKSTPLDVYRILWKPQQETTLPRA
                     PWREELEVRPEGTVLRLVYTLQGEKTRIHAALTRGTLQAAAAVHEMPYREKEIRDLAD
                     RIDSVLSSPDRRGRISKEKLALLKDVARSLYETLVPRALDEFLSIHPVQTLVVEIDDT
                     FAFIPWELLHGRDGFWCLKYSMGRSLAVPDEAALPSRPSGKEPLKMMVVADPRGNLPT
                     IRSEGIGLQKELGKTAGSRVTVSLKGRDGTCGFLRSNLYEYDLFHFAGHTQYDVADPS
                     RNGLLLADGRFEAGWLSSMAQKKPLPSLVFVNACPSGRSAQGSPGLRFTGLAPGFVRA
                     GTRHYVGAAADLAEQSTAAFVETFYSRLIRSQSVGEAMREARLQAISRYGEENLTWAS
                     YVLYGDPDFIYFPRGVAAEARTTTPSTSVFIKRKPVLAAGLLILLGAAAYSLQWSRQP
                     RVIPPPRESVQPPSPVPEVPAPRETAAPLRGPSEKMIPETVVPPEPEISARETLTSAK
                     MQEADTTLEAIHSKMGILQFCFKTSGAFQNSPICRSVCFDIHEKRRQVNVTLEEIRAF
                     ETARESEGGPAGEIRKTMEEVRKRAAAVPNLPPCPDVTPIPPTPVYGLPAAPPGSVYV
                     DDDQGNRTYFDGTGNVLSSIRVRPAPSQVPAAGSAPPSSSRGPSPSTPPPEPGTSASP
                     SGLREASPRGSVGSTIPEPAVQKGPASKRPGADEEKGRVSSFRDTAPKSITSPRDKAR
                     KLPISAEKKGREQQTPALVSPRKQKDKKGPQAKGARRDASESQAAKDEPPSDGRRPPS
                     PRKQRD"
     gene            complement(24350..25516)
                     /locus_tag="PLM79_03725"
     CDS             complement(24350..25516)
                     /locus_tag="PLM79_03725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV85445.1"
                     /translation="MQFIDLGMQQKRIRSNLEDRIRQVLDHGQYIMGPEVAMLEKALS
                     QYTGTAHAVGCASGTDALLMALMAYDVGPGDAIFTTPFTFMATAEVISLLRATPVFVD
                     IDPITFNLDAEHVERALTALVRDRPGDYPLPRGLGDRKLTPRGVIGVDLYGLPADYES
                     LNSLAEKHGLFVLQDAAQSFGAEKNGKKACAMAGIGCTSFFPAKPLGGYGDGGMCFTN
                     NADLAEKLRSVRVHGQGSHKYENVRIGVNGRLDTLQAAILLEKFEVFPEELSLRQRVA
                     RRYGDLLGAEDSPVIPPRIPRDCVSAWAQYTVTARDQKTRSQLQGRLKEAGIPTAVYY
                     PTPLHLQPAYAHLGYERGDFPRSEDCACRVFSLPMHPYLSEEEQEAVAEAILGR"
     gene            26043..28592
                     /locus_tag="PLM79_03730"
     CDS             26043..28592
                     /locus_tag="PLM79_03730"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOV85446.1"
                     /translation="MTATAHSPETKYVCIHGHFYQPPRENPWLEAVEREETAAPSHDW
                     NERIHMECYRANTAARLVDEKNRVLRLRNNYQSLSFNFGPTLLRWMEGHDPESYRRII
                     QADRESHGRSGHGNAMAQAYNHMILPLACLRDQVTQVRWGIRDFEHRFGRKPEGMWLP
                     ETAVSRETLGVLAQEGIRFTILSPYQASQWRLLHETAWRDARDGTIPTGRAYRFDCGG
                     GRVIHLFFYDAELARGIAFERLLEHSSRLLDRIRHGFDHRVCDAGEPWLVHSATDGES
                     YGHHFKFGDMALAAAFQELDRDPSYQVINYGEFLARFPTTAEADIVENTAWSCAHGLG
                     RWERDCGCRIGGDPGLHQKWRAPLRKALNRLRDALALHYEKEMGRLCLDPWEARNDYI
                     EVVLDPEAGREDFLSRHARAGPANSDLLRFFQLLEMQRCSMLMFTSCGWFFDDISGLE
                     SVILLKYAARAMQLARMTGSAPLEDSFLQLLEKAPGNRKEFANGAEVYRKKVLPEIVE
                     LDRVVANHAVRSQARTRGPGYRLYCWQILPQKEEDLGSYPARSLYGHVRVRDTRTWEE
                     ADFLYAAFHFGGLDFRCSVKPYGDDAEYRAILASLQRSMEEQNMVNTVRFLDQTFGTA
                     FFGLQDAFRDLRSSVAVEISREKLRLYRDLQHHFYQTHRPLMISLRQWGLPLPSDVRG
                     AVRSVLSDEVNHIVREILEHQQGCEAEGECWEDTDFHYRVHLGRLNAILEDARWWGLV
                     LRTGDLHDVLGKAMVECLSALGKGFRVEDAGRLARLLAVCERLGVKPETWKLQTLFFE
                     MIAQSLKQPEEYWNKLKFRGFVERLDDSLGCNFARLAPPSPGPSPRSPLAT"
     gene            28753..28938
                     /locus_tag="PLM79_03735"
     CDS             28753..28938
                     /locus_tag="PLM79_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HOV85447.1"
                     /translation="MCEANAYLIKNGREELVMEAVDVVESEGEVLRVANIFGEQKILK
                     ARIRSMSLVNHKIVLEE"
     gene            complement(28974..29735)
                     /locus_tag="PLM79_03740"
     CDS             complement(28974..29735)
                     /locus_tag="PLM79_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOV85448.1"
                     /translation="MKVKLENIGIILHRPRYPENIGAAARAAKNMGIGRLAVVRPEDC
                     DLTRILKMATHNAQEVIEAMEVHDELEDALAGYQYVVGTTARKGSHRQAVKSPRRLAE
                     ELVTLSRSNRTALLFGPEDRGLTNRDLRYCDALVTIPTAEFASLNLAQAVMVLAYEIF
                     LASLDEPKSFVPRLAEKIEMEALYAQLQDILARIHFINPENPEYWMTGIRRFFSRIEL
                     RAREVRMLRGICRQMDWYCEQRLPRLPSGPSEREE"
     gene            30131..31549
                     /gene="amrB"
                     /locus_tag="PLM79_03745"
     CDS             30131..31549
                     /gene="amrB"
                     /locus_tag="PLM79_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOV85449.1"
                     /translation="MRQQKIRESVIAGSWYPGNPETLRQEITRYVNEVPDPRVEGTLL
                     GLICPHAGYMYSGGVAAHAYKLLTGHPFDRVLIVAPSHRAHFPGASLSRVGGYRTPLG
                     VVPLDDELTELLGAHSSLVGYFPNADSQEHSLEIQLPFLQVMLKDFRLTPVLMGEQSP
                     DFCRKLADVLADACRDKNVLLVASTDLSHYHSQGTARRLDQVVLDRVGALDPEGLSED
                     LEQGRCEACGGGPMVTVMLAAKKLGAVNARVLQYATSGDVSGDTRQVVGYLSAAIFGP
                     PPRAAETSREGRGRVGVDLGLSPEEKDALRRIAYEAIRSRCLGTQMPEIPVDLPKLEE
                     KRGAFVCLHKDGSLRGCIGMIEGKEPLHRTVQNMAVQAAFADPRFCALAPEELEKIDL
                     EISVLTPLERIQDPSEVKIGKHGLFIRKGYHSGLLLPQVATEHGWGAVEFLEWTCRKA
                     GLPPHAWKAEDAEIYVFSADIF"
     gene            31759..32298
                     /locus_tag="PLM79_03750"
     CDS             31759..32298
                     /locus_tag="PLM79_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85450.1"
                     /translation="MGIVLDFQKGRRHLLAKRAFRNWGRRFTDQFDEDTSLSALSDGT
                     LGLLIRGGEENLLPIYDLILSVLGMGSGARFYDLEGSEKMYVMDISIFLLDQLRFESM
                     KRLGWVEDFPTRHISLLDLVEQFGEHFSSRRHETPFLSPSHALYGEYEKTFEGDRSLF
                     VRRLIPEALQAYIRESEKS"
     gene            32337..33176
                     /locus_tag="PLM79_03755"
     CDS             32337..33176
                     /locus_tag="PLM79_03755"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOV85451.1"
                     /translation="MPKERFHLLLAEETLRHLERSGRVRRFSVQQRHGFLLGAVLPDV
                     LFYDLPFFSLAGSGRELHRLQKPHGLEFFRQWSEKAKGRYSPAALACVLGVACHFLAD
                     GYWHGFIGKLCADPPRVGNGFLGHTEKCRHLWLESELESYWMAKLGPPDGYFPVLSGF
                     RRGTGANRTCLTLFKDLLDEAGTESVPTESRIARCLVFQTMLLRQFAQPRWERWRSRL
                     LSARSTRRLGVLLVPPRPTLREPESWEPQREGDPGALCTWDFMGRSVSFLSNRLSMLL
                     PPS"
     gene            complement(33115..35091)
                     /locus_tag="PLM79_03760"
     CDS             complement(33115..35091)
                     /locus_tag="PLM79_03760"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOV85452.1"
                     /translation="MFTLSLRQAALTEAQRQTLQEMWLRCMRRIIVSTTLAGSGHPGG
                     SMSSLHLLLMLFSTLQHRPEEPAWPDRDRLLVSMGHISPGVYSVLCEFGYVREEELFL
                     EFRRAGSCFAGHVECCVPGIEWNTGNLGQGLSAGVGMALALKLRKKKSRVAVLMGDGE
                     QQKGQISEARRFAVKYELNNLFGIVDRNHLQIGGNTNLVMPQAIRADYVAAQWNQIYL
                     EDGHDFDAVFQALKRIHAQEVDDARYPTVLVARTIMGKGVSFMENKAKYHGSALDDTL
                     AGEALAELGLENPIPEFRSRRKTFFDFRPHFCFPRVVPEVRGGEPRTYAPGTVTDNRS
                     AYGAAMEDLARWNNDGDIPRVLAVSCDLEGSVKLDAFHRLSPNAFFEAGIQEHHAATL
                     AGALSKEGFASFFSTFGVFGVCETYNQQRLNDINSTQIKLVCTHVGLDVGEDGQTHQC
                     IDYIGLLENLYGFSIFMPGDPNQTDRIIRYIAAHPGNFFVGMGRSKLPVILDQAGAPA
                     FAGNYTFVPGKADWLREGSQGAILSYGTVLHHALRAGDELKSRHGIHLAVLNMASLRP
                     MDREAVLRAAATGFLLTVEDHHIDTGLGARVALILADEAASCRLVRLGVRNYGLSGKP
                     EDLYRQEDLDAPGIVKTVVKAYSDGWIRKTPSGP"
     gene            complement(35117..36001)
                     /locus_tag="PLM79_03765"
     CDS             complement(35117..36001)
                     /locus_tag="PLM79_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOV85453.1"
                     /translation="MSSCDPKSCDTCPSKDKMSKQDLARCKLARIQHKLLVMSGKGGV
                     GKSSVAAYLALALSNKGMRVGLLDVDLHGPSIPKMLGLHGILNITQDQQILPHEYRPN
                     LKVVSIESMLEDTDSAVIWRGPLKHGVIQQFLADCRWEDLDALVIDSPPGTGDEPLSV
                     ARIIPDARAVIVTTPQEVALADVRKSINFCRKVNLEILGLVENMSGLFCPHCRGFVPI
                     FQTGGGLKTSQEMKVPFLGTLPFDPAVVAGGDRGRPVLEDPGETPFKKAVLDFTERVL
                     QRLSEMKPAAAADPKAVH"
     gene            complement(36892..38217)
                     /locus_tag="PLM79_03770"
     CDS             complement(36892..38217)
                     /locus_tag="PLM79_03770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOV85454.1"
                     /translation="MFRKRNLIAFLTFFLSLTLALPAGTGAVERARSKRSPGTAHPGK
                     PAGDPAAGQINTRSAVLVEVSTGATLLEQEPDLIIEPASFTKVLTLYLVFEAIHQGLV
                     KMSDEIYVSETAWRTGGSKMFVGVGSRIPLEELIKGITVVSGNDACVAVAEHLYGSVE
                     GFVHAMNRKARELGMTRSRFLNPHGLPAEGQVTTARDMAGLCLSYLQRFPESLHYHSM
                     REFTYNNIVQYNRNHLLLKDPSVDGLKTGYVAAAGYHLAATAKRDGTRLLAVVMGATT
                     PGIREREALRLLNYGFRQFVLIQPFPEGKPVAALKVWKGLKDQLDLYPQGPAGFLIAQ
                     AQKNSLRWEVQAPKEVTAPVRANQQLGELVFYVGDQPSRTVPLASREDIPLAGWFKRA
                     WQSILLFPSLDWKVVTGVLAGLSLFGLALLFVASRRSSGPKRPRSPASR"
     gene            complement(38269..39915)
                     /locus_tag="PLM79_03775"
     CDS             complement(38269..39915)
                     /locus_tag="PLM79_03775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOV85455.1"
                     /translation="MAVSQTKQLEFFERLSEHILETLKKLSAERQPLTIQSLHKALTE
                     RAELVDMIGSLPSSEDFGLPGGPPQADPPEGKDLHARIGLLQEQRDQAVRQVDDLERR
                     AGRLADFLRRTVVTLAGLVRPGENASMKKSLEELKRLSLAGAEIEELEAGLGTLKQSV
                     IQEPHLSERPRSGDGGLWGRWMKRRDPSAKPVEISREVHFKDLQDYYREVLRQLGEEL
                     GDSSLERLAEIRQKVNEAESFDHLVGLLADLTSLIQTYTGLIREERKQAAAFIRDIGS
                     SLLQMEARFLSSCSHTSEAHQANRRFDLVLETQMNEIHNSAQLSKSLSEFREVVVNKL
                     TTIRTAMEHKQRDDDIRMEKAKREMEELNKTLRAMKAEIDQIQEKTKSLEQETLTDPL
                     TGAHNQRAYKQRLKEELNRYHRYRQVFSMLLFDIDHFKRVNDEYGHRAGDRCLKEVIK
                     RIRPILRDTDFLARYGGEEFVILLPGIDREAACAVAERLCKMVQNTRFVYQGMEIRIT
                     ISVGVTQATPSDHTEEAVFNRADRAVYEAKNAGRNRVVAL"
     gene            40025..40417
                     /locus_tag="PLM79_03780"
     CDS             40025..40417
                     /locus_tag="PLM79_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85456.1"
                     /translation="MDHHAGVLAATDSPRFAFVGSFLDPRDGEESTLETTNHLVKSWI
                     FQGYESQKSNGESLSPWKRDHKRNLPRGPAGFEWRTFPGSEIFSISSKMEPEPEKTKK
                     VEILARMNEKPAAGDFKLTGEQKNSSSA"
     gene            40469..40771
                     /locus_tag="PLM79_03785"
     CDS             40469..40771
                     /locus_tag="PLM79_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOV85457.1"
                     /translation="MFILANLLFALAKILGLALNIYMWIIIARAILSWVNPDPYNPIV
                     RFLYNATEPVLYAIRKRVPLFFGGIDFTPMVVILILIFLDNFLVASLGGLAVRLSM"
     gene            40792..41286
                     /locus_tag="PLM79_03790"
     CDS             40792..41286
                     /locus_tag="PLM79_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOV85458.1"
                     /translation="MEWTSADIQHKQFRTRWKGLDPQEVQRFLDEIAQEIQRLLGENS
                     GLKKDVQRLEKELQEYKDREKTIRNVLMNAHKTAEQMKANAEKEAKVLIAEAELKAER
                     ILQDAHQRLGQLHEDIAELRRQRVLLETKLRSTVETYARLLDMEKEEEKDDELGSKVK
                     VLNR"
     gene            41313..41486
                     /locus_tag="PLM79_03795"
     CDS             41313..41486
                     /locus_tag="PLM79_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOV85459.1"
                     /translation="MTIQKELLDILACPKCKGSITLNESRDGLICRQCELLYEIRDGI
                     PIMLIEEAKPLES"
     gene            41676..42233
                     /locus_tag="PLM79_03800"
     CDS             41676..42233
                     /locus_tag="PLM79_03800"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HOV85460.1"
                     /translation="MSIPREPPPAKLIVGLLFRDFEIRREALAQLTALYGPLDFATRA
                     EPFSYTSYYDREMGEGILRQVVSFQDLAAADALPRIKHQTNLIETRLSSDAKRRINID
                     PGFLSEERVVLATGKNYTHRVYLRDGIYADLTLLYQKGGFRALPWTYPDYREANLLHY
                     LGALRRKLIFQRKEKVPGDSQGGDA"
     gene            42230..43114
                     /locus_tag="PLM79_03805"
     CDS             42230..43114
                     /locus_tag="PLM79_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOV85461.1"
                     /translation="MISSMTAFGRMQREEAGYSAAIEIRSLNSRSLDIVLRLPKAYLE
                     FEEELRKQVTESFRRGRVEVVVQIEPTAPERKAPRLSKELALYYWRQLHDLQSVLPGA
                     DRPTLRDLLQIPHLYETMETVYDPEAVSRLLRDVMAQSLDQVRLMRTREGEALGRDCL
                     ARLDALEEQIGTIEVRKEPVLREFQERLLRRMTDLLGSTPLSDESRVLQEVACLAERS
                     DINEEIVRLKSHIHQMRVMLRGQDPVDGRRLDFLTQELHREANTIGCKTGDLATVQAV
                     VRMKSEIGKLREQVQNIE"
     gene            43193..43465
                     /locus_tag="PLM79_03810"
     CDS             43193..43465
                     /locus_tag="PLM79_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOV85462.1"
                     /translation="MEIKLLNIGFGNTVIANRVVAIVSPASAPMKRLKEDAKQANKLI
                     DATMGRRTRAIIITDSDHIILSGVQAETIAQRLTSESHPKDANLIK"
     gene            43472..44101
                     /gene="gmk"
                     /locus_tag="PLM79_03815"
     CDS             43472..44101
                     /gene="gmk"
                     /locus_tag="PLM79_03815"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOV85463.1"
                     /translation="MRPYQNGSVRGLLFIVSAPSGVGKTTLIKRILPQWPGLQYSVSC
                     TTRKPRPGEVSGIDYHFLTRDDFLRGIQEGRFLEWAEVHGELYGSHGALVETWLASGK
                     DVLLDIDVQGAGKVLSSYPDAHTLFILPPSMEALRERLEKRGTESPERLLKRLAAASR
                     EIREAPWYEFVIVNDDLEEAVRDFTAVIRACRCRRRPMAPRVREFLQGQ"
     gene            44235..44963
                     /gene="rlmB"
                     /locus_tag="PLM79_03820"
     CDS             44235..44963
                     /gene="rlmB"
                     /locus_tag="PLM79_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOV85464.1"
                     /translation="MWISGIHPVREVLESRGAFVYELMVAREDPRVREVAALAESRGI
                     PLHRVDKHNLDEIVGHSHHQGIALQTEEYPYLSLETLLKPAQETPGFLLILDSIQDPQ
                     NLGALLRSAAFLGVGGVILPKNRSVRVTGAVIKVAAGAAACVPIARVTNLVRALVLLK
                     EAGYWIVGLDAAGEETLYDIDLTVPVGLVVGNEEKGIRPLVRHHCDLCARIPGSGRLD
                     SLNAAAAGAVAMAEVQRQRRSRRS"
     gene            complement(44950..45651)
                     /locus_tag="PLM79_03825"
     CDS             complement(44950..45651)
                     /locus_tag="PLM79_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOV85465.1"
                     /translation="MDPLFRNVSAIQERVRAACARVRRSPDSVRIIGVTKTVSAERIR
                     EGVKAGILSLGENYVQELRRKAEALEGLDITWHFIGHLQSNKAKTAVQWCRWIHTVDR
                     ESLARELDRQAQKTSRKILVLLQVKIGDEESKSGIPAERLPELFSAVEALDGLQVRGL
                     MALPPYEEDPERTRPYFRTLHRLLGHLRRQARAPEDLTELSMGMSHDFEVAVEEGATM
                     IRIGTALFGSRSTSA"
     gene            complement(45801..46496)
                     /locus_tag="PLM79_03830"
     CDS             complement(45801..46496)
                     /locus_tag="PLM79_03830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOV85466.1"
                     /translation="MKPPPMPGESSPFNSRTLPSADVEPPGRDAQGVGVYRTLEPLVL
                     ASSSPRRRQLLESVGLRFEVKPSHCDEPWVPGQEPESQVRTWASRKAAEVSRLNPASW
                     VLGADTIVVLEGVVFGKPGDSREAADTLRKLSNRGHRVISAVCLLHEGYGQAHIRSVE
                     TEVWFKRLEEEEIRAYVRTGEPMDKAGAYGIQGVGAFLVRAVRGSYTNVVGLPLCETL
                     ELLAQQKVIVPAA"
     gene            complement(46453..>46987)
                     /locus_tag="PLM79_03835"
     CDS             complement(46453..>46987)
                     /locus_tag="PLM79_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV85467.1"
                     /translation="KALAAGPRVVALGEIGLDFYRDRQPRSSQTRCLRQQLELAVELK
                     SPVIFHIRDAYEEFLRVIGDYPSLRGNGVMHCFSGTWDIAGKCLDLGFYLSVPGTVTF
                     SKAETQQQVARNIPLDRLLVETDAPYLTPVPYRGKVNEPAYVRYTAQKIAELRNSTLE
                     EIAHETTANARRVFSIQ"
BASE COUNT         8883 a        13888 c        14687 g         9529 t
ORIGIN      
        1 ggagggcctg aacccatcgg cctgcccggg tcgggaaaca tcccccgggc aggccgagtc
       61 tttttttggg gacttttcgc tcttcgtgga gcccctcagc actcgcttcg tggagaggca
      121 cgacgggagg agggcgagct gggaggttcc ccctggggcc gacgagccac aaaatgtctt
      181 ttcaaaaggg aattgaggag gatctggagg aaatgcacga ggctgagaaa tgaaatcccc
      241 ccgaccccgg gccggggtga ggggggaccc gaactgcgtt ggcgtttggg cccttgacgg
      301 gagacgatcg gcaagaggaa gactcgcctc accggaggga accttatccc ttctttcgcg
      361 ggcgcagagg gctgaggatc cggatgaccg aagccatggc cgcggagcac tccttgagaa
      421 tgagcatcat gatggagaag gccgcgaaat tgaggacggc gcatgcgacc caggcaccgc
      481 tgagggctgc cgtgcccgcc aggagagcga acccgaaagc aatcacaatc ccggcgggag
      541 cgcatcgggg ccaggcacgg aatcgaatca attgcttgcc cgctccatgt tcctccacgg
      601 ccatgaggat ccgcacccgg cccaggagac ctccgcggag ctcgagatcc cagtggtcgt
      661 agtctcctcc gcgcaaaacg acggtccgaa gcttcttgag gctcccttcc agggattcca
      721 gccactcgag gggggacctc cactgttcgc tccagacggt gaaggagcgc tgccagggga
      781 gcggcagagc gactcccggc accgcacagc gacgccacgg ggtgagaccg gcccgaagtc
      841 tgcccatgag ccgggccagg ggttgaatca tatggaggaa ggcggtgagg gcgcgcagct
      901 tgagacggct cttccctgcc cgggatccct cggggaacga aacccgcaaa gcgctccaac
      961 aggcgtgaac gaaaggaacg gcggccgcca ccacgaagac cgggaatgcc gccagcaggg
     1021 gagcccattc gagtcccagg agggacaggg atgccagggc ggccaccacg acataccatt
     1081 cgggcatcat gggcagggac cacaacaggt cgggagcggg ctgatagatg gactggaaaa
     1141 gagcggttcc ccaagttccc tggtaaatcc gacctcgaca ccatcccaac agctgctcga
     1201 ctcctctcag gtacagcctt cccgcccatg ggacatgccc tgcggcgttg tatttctccg
     1261 gccatttctt ctcgagatcc gcttcggcct ttccgtaatt caactgctgc ttccagtaag
     1321 ccttcacgga gtttcgacgg tgatgccaga ccacggcagc ggggctgaac cccagtttcc
     1381 atccctgttg ctgcagccgc cagcagacat ccacgtcgtc tccggcgatg cggaaccggg
     1441 tatcgaatcc gtcgatggct tccagggccc atttgcgaaa ggacatgttg cagccgggaa
     1501 tgtgctccgc ctcgcggtcg gtcagcagca catggatggg acctcccggg gcgttggcca
     1561 cacaatcggc aatgggaccg tcggtgggcg gcgccaggtt cggccccccc actcccacga
     1621 agtccgactt gagaaaggtg gcggccaggt aggtcagcca atggggatcc gggcgggcat
     1681 cgtcgtcgat ataggcgacg atttccccct tggcggcgcg catgcccgtg ttcctggcgc
     1741 tgctcagccc gcggttttcc gtcttgatca tccggaaccc gtactcccgc acaacctctt
     1801 cggtgccgtc ggtggagccg tcgatgacca cgatgacttc aaagttcggg tattcgacct
     1861 tcttcagtcc ttcgagggtg tcgcggatgg tccgctttcc gttgtaggtg caaaccacca
     1921 ccgagatgcc gggccaggga agatcgggcg gaaacgggac ctcggagaaa gccttccgaa
     1981 cggcgctcag ggacgtcttg gggttgcgtt cccgatccgt gatgccgaat ccccagtcga
     2041 ggatctcatg gccgccccgg aaccactcgt cggtccacga gaatacgaaa atcccggcgc
     2101 atcctgccgc gaacgtcgat cggatttgcc aggaaagggt cgcgccctgt ttgtcttccc
     2161 cgtttcgaat gctgtccagc cccacctccg ccatcaccag ggggcgattt ccggccaggt
     2221 tttgaagacg ggccaggtag ccttccagct tctcccgggt ctccaggtac acgttgaagg
     2281 acacaaagtc cacaaagggc aggtccaggt actcggtggt ggggaagttc acgtaggtga
     2341 ccagtgcccc cgggtcttct tccttgaccg cacggtacag ccgctccagg aaagcctcga
     2401 tcttgcggcg accgtaccag cgcacgatgg aagccgggat ttcgtttccc acggtgaagc
     2461 aaaggagggc cgaatggccc gcacactgcc ggaccccctc ccgcacccgt cgctcgatgt
     2521 cgttggccag tttcttgtcg tccaggaagg ccacatgctc ttcccagggg aggcccacca
     2581 tgacccgcag gccgtgacgc tgggccgcgt ccagcagcca gcgggggggc accgtatagg
     2641 ttcgaaccga attgaggccc atggaagcca tttgggtgaa atcccgttcc acctgttcgg
     2701 gggtgtgata ctcggacccg tcctcttcgg gacggaaggg gccgtaggtc accccgcaga
     2761 tgtaaaactt gtcttcgccg acatacagaa acttgccctg cacccggggc cgcacatctt
     2821 ccaccacggg gatggatttg ggcgggtgca gatagaaccg ggcaacaccg gtggtctgct
     2881 gattcctgcc gaagaccggt cgcaaaacgt ttcgctcatc atgagaactc ttgagaaaag
     2941 cggccggaag ggcctgcgag ttcgccgaaa aatgcggaag agggtcctcg agtcgagggg
     3001 taccgttgga aggaacggcc ggaccgccgc tgtccgccga tctcgtctgg gagtccccat
     3061 cccaaggagc gctcccgggg agaatgtccg tcatcggatc ctcagagaca tccggaatgc
     3121 ttttcaaggc ttgttttgcc ataggtgcct catctgcgtg agagttgatg gtttcaatcc
     3181 gaaaagatcc atggtcgagc cgcgccgtcc atgggagacc tgcgacatcg gcgaaacccc
     3241 ataaggcaat tcccgtgcca tgaggcagaa gctcgtcggg taaggcaata ggagcacgga
     3301 cacggaaagt cccccaggga ggagtacaat tcttgcgctc ccaattacga atcttgcaca
     3361 gaggttgttc ccgccgtccc tgtccacctg cttccatatc tgcttgaaat gacgtgaatt
     3421 cctcgctcct cggccctacc tttggctgta acgatcatct ggatgggcac cctgcatgaa
     3481 agggagggcg gaacctggac gaaatacaaa tttcgatgct gagaaaccgg cttctcgaga
     3541 gacgcaatga gatcctgggt aggatgcgcc ggctggaggc cgggttgcac gaactgggcg
     3601 aacgggaaac ggagatcgaa gaggaggccc ggaaatcgag cattcccgga ctgtacgaac
     3661 gattggacga agcgggcaag aacggaatcc ggaacatcga ccgggcgttg gccgggattt
     3721 cctcggggga ttgcgggatt tgtgaatcct gcggggacga caatcccttc aaacgaccgg
     3781 aggccatccc gtgggccctg ctgtgcgtgg actgtgcgcg tgaacgggag aatgccgccg
     3841 ggtccatttc cggaaagggg gagcctttct ccgaagatcg gactcccggt tgttccccgg
     3901 ccggaaccga gcgggcaggg agtcggtgcg gtctacgaat acctgcgcat agacgggcgc
     3961 gtggatgccg aggacctcat tctcgatgag atcgacggga gactgaccct tgacgggggg
     4021 ctaagaggca gagtccagca tcaggtcctc ctgcaggttc tcacccgtgt tttcggatac
     4081 gcctccgttg tggaccgcct ggaagtcggt gaaagggatg ccgaagagga gcaaaggccc
     4141 atctccggca tcttgggaga gaggagcatc gagggttccg ccctggaggg gcggagcggg
     4201 tttgattaga accctagaag aggaattccc cgcgcaccat gcctgcgaaa atgggatcgt
     4261 tgcccgagtt gccccggggg tccaccaccc actgaaaatc cggggttacg gcaaagtatt
     4321 tggtcaggtg aatccggtag taaagttccg cgtgaacctc ggtgccggtg tggcgaaggt
     4381 cctcgttggc tttcaatccc gccaggccga ttcccagctg gtcttcggcc ctcaggggaa
     4441 ggattccctt ccagttcact cccgcagacc aaaaccaggg gacttcgtag gcatcctcgt
     4501 tgtgatatcc gaaccggccg aacaggccca cggcgtcggt aatcttctgg tcgaggctca
     4561 aacccaggcc ccatccctga accgaacccc tgctcccgat ccggggatga tcgtacgtgg
     4621 cgttccagta atagaggcga tagttgcctt cctgtccgaa aatcttcgga gagtaggcga
     4681 cttgggcggc cacgaaaggg tcctcgagca ggttgtggta catgtccttc tggccggacg
     4741 gcagagcgta cgggtgggtg gacgactgga cgagggcggt gagggtcact ccctccaggg
     4801 gggtgaagga tgcggcgaag atgggtccgt attcgtcttc gttgctgaga atgggattgt
     4861 tgacgaaggg ctttccgacg aactgggaat attggtcgtt ggcaaaggcg ttggtgtcga
     4921 tgaaaaccag gggttccgtc ttgccgacgg caaggatgag cttctcgttg cagaactcgt
     4981 gggcatagta gccttccaag agaatccggt cgaacccgtc gtcatggtga taagggccgg
     5041 aatcgtccgc aattgtgttg aggttggcaa agagcttgct ccccaggcgt tcatcggacc
     5101 ccttgccggt tcccgcgtgg aacctcagga agaactggcc gtccttgaag aaggtggatc
     5161 gtggtcggta agagagtttg aggtccccga cgacgccggg ttcggcatcc tcggtgatct
     5221 ttcgcttccc gatccgttct cccacggcgc cctggtagaa cgaaaccacc gccccgtgaa
     5281 tgtccagtcc gtactctttc tctttcagtt cttccttgac ttcttccagg gtggatctca
     5341 cttcccggga cccctccgcc tcctcttgct tttccttttc ggcgggctgc tttgagaccc
     5401 gggtcgtcgg cgaggacaaa gcctcccggg ggcgctccga ggcagggtcc ggcaccccgt
     5461 cctccgaggc tccctcacaa accccggccg ggcaaaccag cgcggtcccc accagaagga
     5521 ctaccccaaa catccatcct ctcaacacac atgaaaacat ggttccctcc gcaaccgttg
     5581 gtcatcgaga acgaacgagg aatgttcccc gcttccgttg gagtatccgc atggcccgaa
     5641 agaaacaaaa gccacgagca ctccaaacct catgggtcat gaagccttcg tggctttcgg
     5701 tcattgtgcc gaagatgcgg ccggagaccc ggattctttc ttcaaggcaa cctgaacctt
     5761 catggtaaag gtcatccggc aaacccgctt caaatgcctc cgtccagccg ttcctatact
     5821 acgagccgtg aagaaaaaaa agtcatttct tgccggaaga atccatcgtg aagcgccatt
     5881 tgcagaaaaa ttcgggcggg cgagggtccg gaggagcgta gacgcattcc acccggatgg
     5941 cgggatcgat ttcatgggcg aagcgggtga attcgtcccg gtgcatgtcc ttgcacgcgt
     6001 attctcccag gccttgcttg attcgcgcct cctggggagg gcaatggggg acggtgagaa
     6061 tgacctcccc gggattctcc cggatctggt agcccacgat cagggtccac gggaagagtc
     6121 gaagggcctt caggaaaccg gccagcccct tttcctcgat ccggaacctc ttgcgaaggt
     6181 cctttgccgc catccccgcc accttgcccc agacccgttg gttgaggtgc tcggcctggg
     6241 caagtccgtg gctctcttcc acgtacaaaa accagaacgc atccatgacc cgataatgcc
     6301 agagaaggaa ttcgagataa tcgagaagct ggtcccgttt catggttcta agaacttcca
     6361 gttccatcgg atctccctgt ctgttgggcg gaaatgaaag gcgtgccgtc ccggaaaccg
     6421 gcccccgagg ttggaatcat agcggaaaag gtcctcgggg ttcaaaccca aacagcggtg
     6481 cgtcgaactc ttccccgtcc cgaacaaggc ccgtgccgag tctttcatcc ccggagccgc
     6541 cgtgatatcc tgatccggaa cgcagatcga gggaggaagc gggcgatgaa gcaatggaac
     6601 gagctcacca cggcaggggg cacgggggtc caccccgcag gacccatcat tgcggccaga
     6661 ggtctgaccc gggtctaccc cctggaggcc tgcgaggtca tcggcatcaa cgaggtggat
     6721 ctcgaggtgc gcgccggaga actcgtcgta ctcaagggag acagcggttc ggggaaaagc
     6781 actctcttgg cccttctggg cgggatggac cgccctaccc gcgggtgcct gatggtggcc
     6841 ggacaggatc tttccacggc cggggtctgg gacctcaccc ggtttcgacg agaggtggtg
     6901 ggaatggtgt ttcaatcttt caaccttttg ccgaccttga gcgccttgga aaacgtctgt
     6961 ctgcccgccc tgctggccgg ggtttcctat gggaaagtcc gatccaaagc catggagctc
     7021 ctggcctggc tgggattgga atcccgggtg catcaccttc cggggcaact ctcgggaggc
     7081 gaaatgcagc gcacggccat cgcccgggcc ttgatcaacg atccgcccct gattctggcg
     7141 gatgagccca cgggggacct ggacagcgcc aacgggcacc gggtcatgga acacctgggc
     7201 gaactgaacc gcaggcaggg acgaaccgtc ctcatcgcca cccacagtac cctcgccgac
     7261 ccctatgcga cccgaacggt gcgtctcaag gacggcggca ttgccgagat gacatgggcc
     7321 gactgactct tttcctgcga ctctggctct ggttcagttt tcgacacctg cgggcccatt
     7381 accggcgcac cttggccgtg gtggcgggga ttgcgctggg tgcggccgtc ttcaccagtg
     7441 ttcgactggc cgttcacgcc tccattgatt ccttcactcg aaccgtggat accctgacgg
     7501 gcaaagccga ccgggtggtg atccatccgg gagggcgggt ccccgagtcg gtggtctccc
     7561 aactggcgaa gcacccgtgg gtggagagcg catcgccggt gatgaccacc tacgttcgtg
     7621 ccgtggaaag atggacggga cccttcctgc tggtggggat cgatcccatc ctggatcgtc
     7681 ccctgcgaac ctgggaaacc cgggaccggg acgtcgcctc cggcttgcgg gcttccgcct
     7741 ggatcgatct tctcagggaa cccttttccg tgctgctggg ggatggactg gcccgtcgcc
     7801 tcgacgtgcg ggagggaggc cgggtggaaa tccaggggcc ccgggggaat acgccgtttc
     7861 gggtcgtcgg gatcctcgag tcccggggat tgggggcggc ggaaggggga gagacggccc
     7921 tggtggacat cgccaccttc caggagttca cggggcaacc gggattcgtg gaccgggtgg
     7981 atctcttgtt caagcccggc attccttccc aagaggtgga gagcctccgg gccctcctgc
     8041 ccgcagccct tgtcctggaa atgcccggag aatcccggga gagcggcagg ctcctcattc
     8101 gttcctacga gctcaacctc tcggtcctga gcttcgtttc cctcttcgta gggatgttcc
     8161 tgatctacag cctggtggcc ttgaatgcgg cgtcccgcag gcatgagctg gcggtcctgc
     8221 gttgcctcgg agcttcctca cgactgctct tcgtgctctt cctgttcgag ggggtgtttt
     8281 ttggagtggt cggctggctc acggccgttc ccctgagcgg cttcctggtc ggaaggatgc
     8341 tccacggcgt cagcgaaacc atcagtcttt tgttcgtacg cgttcgggtg gaccaggtgg
     8401 ggctcgattc ccgggaagtg gccctttctc tcctggtgac cgttctcatt tccatcctcg
     8461 ctgcatggca gcctgcccgg caggccatga aggtccctcc ccgggaagcc ctcctgatcc
     8521 gggattccct ggccgaaaga aggggttccg cccggaaaat cgcctttctg ggaacggtat
     8581 tgatcctgct ggtctggccc ctgtcccaac ttccgggcat cgccgggatt cccgtggggg
     8641 gatacctggg aacctttctg ctctttgccg gattttccct cgtttctccc tgggttttga
     8701 gcctcctggg ggacaaaatg cccgaggtgt tgcgcaaggt cggaggagaa ccgggattcc
     8761 tgggagcccg gaccattcgc gatgcggggg cgcggatcgc catttccgtc ggggcgctca
     8821 tcaccgctac cgccctgttc gtggccctgg tcatcatggt gcacagcttt cgacaaaccg
     8881 tcacggcctg ggtgaaccag accatcaccg gggacctgtt cctgcgcccc aggatggcgg
     8941 agttcaacca ttatcgggac cccttgcccc gggcggcggt caaaaccctc aaggaactgg
     9001 aaaccccggt gcaggtcctg ccctatcgac ggatctttct tcaccagggc aacatccggt
     9061 accagttcga agccatcgat tttcccgtct ttctacgcta cgccaagttc ctccttctcg
     9121 aggggaaacc ggaggaaatg attccgaagc tgacggaggg cgagggggtg ctcatttccg
     9181 aagtattcgc caatcggtct cgactcaggc tgggggacac ctaccgggtc cggatccgcg
     9241 gggtggagat ccaccggccc gtggtgggga tctttcggga ctaccgaacc caggggggag
     9301 tggtccacta ttccttgtcc cattttcagg aactgacggg agacgattcc tggggcgggg
     9361 ccagggttca cctcgtggac ccgggaccgg acccccaagc ttcggcggcc cgggtccggg
     9421 aggagatcca tcgtcggtgc gtcgaatgtg tcccgggggt cgaaatcacc ttgggcaacg
     9481 acttgcggcg aggggtcctg cgaatctttg acgagacctt tgccattacc accgtgctgc
     9541 tgttcattgc ccttttcatc gccgccctgg gaattgccac gaccctgatg gtgctggttt
     9601 tggaaagggc ccgcgagttc catatcctcc ttgccgtggg tgccgaacgt tcccaggttc
     9661 ggcggatgat cctgtgggaa gcggtcctca tggtccttac gggggaaatc attggcctgg
     9721 ggtgcggttt ctgtctttcc tggattctcg tgtacgtcat caaccgccag tccttcggat
     9781 ggactttcgt gtatgccgtg gactggagct ccctgctggc ctcccttccc ctcgtgctcg
     9841 ccgccgctct gctggccacc ctccccgcaa gccggtgggt attccgtcgt tccccggcgc
     9901 agatcttgag gcagggctga gaaaggaact gtgcgaaggt cccatgagaa tgcgacggtt
     9961 ctgcggatat tcgacgacgg tgctcctggc cgtgtgcgtg gtgctgggcg gcctcgcccc
    10021 ggccggagga caggaggggg aatacctccc ggtaacgggg ccctgcggac tgcagtttcc
    10081 ccgggaccac ggttcgcatt cgggataccg caccgaatgg tggtactaca cggggaacct
    10141 cgagaccccc tcgggaaggt ccttcggctt ccaactgacc tttttcagga ggcaaatcag
    10201 tccgccgggg ttcgagaaac actggcccga gcccccttcc gcctggcgga cacaaaatat
    10261 tttcctggcc cacgcggccc tttcagacct ggagaggggg aagttttacc aggaggagaa
    10321 ggtggcccgg ggggccgtcg ggctgtcggg ggtgactcgt gactcagggg agacgcgggt
    10381 ttttgtggaa gactggtccg cagggatccg ggaatcgggc cacatcttga gggccgcaac
    10441 gggggatttc tccctggagc tggctctggt ccctcaaaag gccccggttc tccacgggga
    10501 tgcggggtat tcccggaaag ggggcgggaa ggaacgggcc tcgtgttact actccttcac
    10561 ccgtttgaag gccgaaggca ccctgaagct cggggaagga acaatcctgc ccgtgcacgg
    10621 cgaggcctgg atggatcacg agtttgcctc ttcccccctc gaaccgggta tcgtggggtg
    10681 ggactggttc agcctgcaat tcaccaacca aaccgaactg atggtctacc tcctccggaa
    10741 ggccgacgga gggttcctac cccagtccgg gggaacgttt gtgacccggt ccggggaggt
    10801 ggtccccatc gcttgggaag acctgcgcgt ccgggtggtc aaagaatgga agagtcctcg
    10861 atcgggggcg cgatatcccg ccggctgggt tcttcacatc gggtcccagg acctggaagt
    10921 ggtggtgacc cccaccctgg aggaccagga gatgcatacc cctctcagta cccgggtgac
    10981 ttactgggaa ggaagcgtgg gagtcgccgg gaccatggcc ggagctcccg tcaaaggcaa
    11041 agggtacgtg gagctcaccg gctacgaccg ccccttggac gcccttcatt gacggccgga
    11101 gttgcccatc ggggttttgg aacgcgtggg aaagactcgt ctccgggacc ccttcgtttc
    11161 tccaagggat cgaaccgtct cccccgcagc ctcactaacc gacgaaaaag cccctccccg
    11221 cgacggggaa gggctgagag agtccggtgt gctgagaggc gatccctatt cggggttcac
    11281 cgccacggcg gccgtcgccg gttctgcggc cggggcctgc tccttgaaat aggcttcctt
    11341 caccttcaac aggtacagga cgatgggtcg gtaagccaga tgggcccact tggcaaaggg
    11401 cacctcgagg accagcatgg gaatcgccac catcatgtgg acgatgtaca ggaagtaagt
    11461 ggtggccggc cagtccagga gccgggtgaa gtggatgaag atccccgtga gggtcgtcag
    11521 ctgcaggaga atcaggaaca tccaatccgt gggatgggaa ttcttgtaca cgggcttgct
    11581 cttcttgagt cgcccgatga tggcataggt ggttccgtac atgatggcga aggtggcata
    11641 gtagcccagg aggcggattg ggtgccacag cgggtattcg ggaaggtccc gctggaactt
    11701 cagggtctcg acggtaagac cgttgaccag gaccacaacc atcaggaata cggtggcata
    11761 gcccgtcatg atcatcaggt gcacgaacca ctgcatccgg tcggtgcatt ggccgaactt
    11821 tttctgggtc agcacgtgaa tgagaagctc cttggcttcc cgagcgtaaa tccccagcgg
    11881 caccttggtt aggagatccc ccatgacgaa tttcgcacat cggagaatgt tggtgatgag
    11941 gaaaaaggag aggatcgccg ccatgacgag gtctgcgatc tcgatcttgt ccgaaggcca
    12001 caccgaattg atgtgggcgt gttcccaatt ggggctgccg gtattgaaaa gaagcaggcc
    12061 cacgcccacc aggattgcca cgatggtgat cgcaacgatt tcgaattttt ccgacgtgta
    12121 gaacttccgg gcaaaccccg tccagtcata gagggaagtg aggtagcgcc tcatgaccat
    12181 catggtctcg ccggggtccg cgcctcgggg gcacttggcg gagcagtcgc cgcagtagta
    12241 gcaaagccac ggctcgggcg catggaggat cttgtccttc tgccccaact gcacgtagcg
    12301 catgaagcgc cgcggaaacg tgttttccgc agtggagagg gggcaggttg cggtacagtt
    12361 cccgcagtga taacacttgg cggcatcctt gagaccgaat gccgttaagt ctttcatgag
    12421 attcggatca accttgttgc tcatccctgt gcctcctgat ttgatcgtat ggaaaatgtg
    12481 tgaaaacctc gtccggcaat agcggttcgt gaaatcagga gtcgaagagc cgaggggtcc
    12541 ccggggttca ttcaactcct tgttgacgga caatgtgact gttcccgggt cccgtgcggc
    12601 agccggtccg gcgggcccgg tggaaatctc gtgtaggccc tctccccctg cgccggaggg
    12661 agagggccct ttcgccaacc gtttgcggga ttcgaactag taccccttgt aggggttggg
    12721 tccaacctcg acgaggcgtt cggcaaagga atccaggagg gcagggagcc tgtcgtaatc
    12781 ggtgatggag acggtctcaa accgaactcg atcggattcc agcaccagcc gatccaaagt
    12841 ctccttgacc ttgctcagcc ggatttcggc cagctcgctt cccttgacga agtggcactg
    12901 gtagttttcc ccgtggcggc agcccaggag aagaatgccg tcgatgccct tggagagact
    12961 gtcggcgatg aagacgagat tcatggaacc caggcagcgc atggggatga cccgcaccca
    13021 tgcgttgtac gtcatgcggc gaagcccggc catgtccagg gcgggatagg cgtcgttttc
    13081 acaggccaga acgagaatgc ggggcttttc ctcgtcttcc tcgggaacct cgatggactt
    13141 gatcatgtcg ccgatcattc ccacggagta gttcttgaag gagatgattc gctcgggaca
    13201 ggcgcccatg cacacgccgc accgacggca ccgggtgggt tgaggcaggg gattggcctt
    13261 ctcgtcttcg ttgatggctc caaaggggca ttcctcggtg cagcgcttgc actgggtaca
    13321 tcgctgcatg aagaattccg ggtaagtcat gtccccggcc cgggggtgaa cggccgctcc
    13381 ctgcatggtg agctcgatgc actggatggc cttcatggcg gcccccgccg catcttcctt
    13441 ggtacttgcc gtacccatag gacggcgcac gcacccggcg gcgtagatcc ccgtgcgcct
    13501 gctttcgtac gggaaacaga tgaagtggga atcgggaaaa ccgtacttga gacccggaag
    13561 ctcgggaccc tggcggtact cgaggttgag cacgtccgat acccggatgg cgtctgcggg
    13621 gacctggatt tcttcctcgc cctccttctt ggccgccgct ttgaaatctt ctccgaaggc
    13681 ggtgacgggg accattccgg tggcaaggac caccatgtcg aggttctcga gaaggacctt
    13741 ctcccccagg agttcatcgt gggcttccac cgaaagcccg ccgacgccgg cacgcacctc
    13801 gggatcctgg ctgcggatga agatcacgcc ttcctcctgg gccttgcgat agaagtcttc
    13861 ggcctgtccc ggggtccgca tttccttgta caccacgtac acgttggcgt cgggatcccg
    13921 gctcttgatt tgaacggcct gcttcaaagc cgtgaggcag cagatgctcg aacaatagga
    13981 caggtgttcg gggtcccgct gacccgcgca ctggagaaag gccacgttct tgaggggcgc
    14041 cccgtcgctg ggtctgacca gggttccccc cttggccagg tcttcgaaca tgacgttggt
    14101 gacgacgttg ggggaggcgc cgaatcccag ctttttatcg agcttttcgg gatcgtaggg
    14161 tttccatccc gccgccaaaa cgatggcccc gatggagtgt tgcacactgg tgccgttctg
    14221 ctgaatggtg acctcgtaga cgcagggacc gcccacgatc ttttccacct tggccccggt
    14281 gtagaccgtg atgctctttt catgggagac ggtccggatg agttcctcga ggtcgttgct
    14341 ccggacctcc ttgtagggag tggtgacctt gagggaaacg ttcttctgaa acccgcccaa
    14401 ctgggcttcc ttttccacca ggaccaccga atatccggcc cgggaagctt cgagggccgc
    14461 cgtcaatccg gccaggccgc cgcccaccac caggatgtcc cgggacattt cctgttcggg
    14521 tttgtacggc tggggaagct ccgtcttctt caccttggcc ccggccatgc gcagatagtc
    14581 ttcggccatc atctgggtgt cttcgtcctg gggcttttga caccagacca cctgctcgcg
    14641 caggttcacc cgctccacaa tgcaaccttc aaaattgaac acgtcgtaca tgacccgggg
    14701 agaacacgcg gcgatgatga gggtgttggc tccctcttgg gccgcgtcgc ccttgatgag
    14761 gtccaccccc tcccggctgc acaggttggg gtgattcctg caaatggggg ccttgcattc
    14821 cttggtggcc accttgccga gggcctcgat gtccagggcg tccccgatgc cgcatccggt
    14881 acaaatatat actgcgatct tctgctccat agagtttacc tcctaacgat ggattgaatg
    14941 gctttgagag acgcggccgt gccgtcctgg accgattcgg ataccccggc gggggtcctg
    15001 gtgcagcccg caccgtagat gccgcgaccc gcttcgatgg aggccacaaa cccataatcg
    15061 tcgtaggaga cggctccggg gaccggcgat tcggcggtat tgggctgcat tcccgtggca
    15121 aggaccacca tgtccacggt gatttcctga agggacccgc tggtggtttc ttccacccgg
    15181 agggtgaggt tcttagtagc ggggtcctcc tcgatcttgg ccactttgcc cttgaaaaac
    15241 cggacgttgg gatcctcctt gaccgagcgg acgaaatctt ccaggcggtc cgtggcccgg
    15301 aggtcgatga agaaaatgta catcttggct tcgggatact gttcccgcac gtacgtggtt
    15361 tgtttcatgg aagccaggca acagatggtg gaacagaagg gcaggtggtt ttcatcccgg
    15421 gacccggcac actgaacgaa ggcaatggtt tccggagcct tgccgtcgga tggacggacg
    15481 atcttgcctt gggtagggcc gttgtaggcg gccaaccgtt ccatcatgac gttgctgatc
    15541 acgttggcgt aggtgccgga accgtaggcg tccagcttgg tcatgtcgta aggcttccag
    15601 cccgtggccc agacgatgga gcccactttg aggtccagga ccttggactg catgtccagt
    15661 tcgatggcgc cgtaggggca ggcttctttg accttctgcg cttcggggct tttcaccaga
    15721 gagggatcga gaacgtatcg gaggggaaag gccatgtcgt gggggagata agccgccttg
    15781 atcttgtcca tgccgtagtt gaacgggttg tcgatttcca gggagcaggc ctgggcgcac
    15841 aggtcgcagg cggtgcactt ctcgttgacg tagcgcgggt tcattcgaac ggtgacgtcg
    15901 aaattgccct cggaccctga aatcttttcg acttccgcca gggtgatcac ccggaccctt
    15961 gggttgttct tgatccggcg aaaattgatc tccagcccgc aatagggggg acaaagcttg
    16021 gggaagtact gatgaagctg agccacgcga cctcccaggt acggattctt ttccacgaga
    16081 tagacttcgc ggcccgcttc tgctgcctcg acggcggcgg tgagcccgct catgccgcct
    16141 ccgaccacca gaatactctg tgttactgtg ttcgacatgc tgatcctcct aagctgaaaa
    16201 gaaatatcga aaacccctcc cgcgttcggg tgcaatctcc gcggccggac cttgtgttat
    16261 ttcgcacgag cgcagggctg tctattcggg cttaaatatg tgcatgtata cacaaagtcc
    16321 ccccgccttg caacaaaaag atcaaacccc cgggattcct cccatttgtg tgggctttta
    16381 gtggatttcc tgggcatcgt caaggtccgg cccaagaaat tgtgaaaaaa aggtcacagc
    16441 ccctcgctct tctccttgaa aaatgacggc aagtccttta ttttgagttc tcgatggtgc
    16501 ggattctcac aggattcaat tcaacagacc caaggatgga gtagccggat ggaagaaaaa
    16561 caagatctca gaagccagtg gttctcttac gggataaagc tggcgttgtc ctttgccttc
    16621 ctctttcccg tgtattttct cctctatgtg acctttccgg gttccctcgt ggcgctgtat
    16681 ttcatctcgg cggtgctctt cgccgtattt gccccctggg ataccctgaa gcagaagttt
    16741 ctctcctgat cgccaacccc tcagcgtgca attcccggcc cgtcccctca gggcccttcg
    16801 aaccaaggaa aaagtgccgg cttatccagg ccggcttatc tttctgccaa tcttcccttt
    16861 ttcaggtggg tctgccgggt gaacaaagga ggtcctgccc ggccgtttcc ttctgcttgc
    16921 gaagcgtcgc ccggagcagt tcctcggcct tttcgaactt tggatcgagt tccagggcct
    16981 tcttgaggga ccgaaccgcc tccgcgacgt cctgccggtt gaggtgaagg actcccaggt
    17041 tgtagtggat ggcgggattg ttgggtcgag tggtcaaagc gagccggtag tagcgttccg
    17101 cttcctggaa cttcccctgc cggcgcaggg cgatccctaa ccgattgtag gcttcgacgg
    17161 aatccggatt gctccgcagg gctcgtaggt agagcttctc cgcttcggca taaaggccgc
    17221 tttccaggta aacctccgcc acggaaagac ggtaaccctg cttttcttcc ggcgcggccg
    17281 tccgcaggac gccctcgaga acactacggc cccggtccgt ccgtcccgcc tgaaggagaa
    17341 gcctgcccag agtcactttc cgatccggat ccatgggact gatgcagttt accttctcca
    17401 gcgcctcgac ggctttgtca atgttgccca agttttggtg gacggtcgcg taattcttgt
    17461 acgcagccag gaaaacgtcg gaaaccctca tcaccttctc gaagcagacg gcggcggatt
    17521 ccatgtcccc caattccatg aggcattcgc ctttgagatt cagccatttg agcttgaggg
    17581 aggcaacgga atgttccagt tgatcaatgc gctccagaac cgaccgggcg tgtccccttg
    17641 ccttcctccg ttccatttca cgaaagagaa gctcttcggg gtccttgagc ttctccagga
    17701 ttccatcgac ccgggacttc aggtccatgt aggagaaggg cttgatgatg taatcgtagg
    17761 caccccattc ccccgcactg gcgacccatt ccggaagcga ttcgccggaa atcatcagaa
    17821 aggggagccc tttcaattca atgtgggtcc ggcatcgctt gaggagcttg atgccgtcca
    17881 ttcgaggcat attgacgtcc gaaacggcga gatcaaagtt gccgttggcc agcttcgccc
    17941 aggcttcctc tccgtccgat gcgtcgtcga taagctggaa gtacctcatc tgttgcagca
    18001 tcttcctgac tacgctgcgc atggccacgt gatcttccac caccagagct tgcagcgaag
    18061 cgttcaccgc tttcctctct tctttctccg ggtgagcgaa tgggttcccc tgccgtgtgt
    18121 ggaaactccc tctcaatata cggtccattt caggaaaacc tgaagaagaa gtgttggaag
    18181 gccctggaag gaggcaaagc cccgggtcat ttctcagggg gatgccttgg gtccggggcc
    18241 gccgggggta gggtaggcgc cccgggatcc tccgcggcac tcgacggcct ggcctgccgg
    18301 tcgatggttt ccgggaactc cgggtcttcc ccgggaaaag cccccaattc cttaaacttc
    18361 ctggcagtca ccaggacccg gctctcggcg gcccccaccg cccggttgta ggcttccacg
    18421 gcgcggtcca ggccttttcg caaatcggca aaatgggcca tccaggagcg gatccgatca
    18481 taaagggtct ttcccagttc gctgatggcc tgggcgttcc gggccatttg ttcctggcgc
    18541 cacccgtacg ctacggctcg aagcagggcg atgagggtgg ttggggtggc caggatcacg
    18601 tttcgttcca ctcccgcctc gatgagggag gggtcctgtt cgagggcggc actgaaaaag
    18661 gtttcccccg gtaagaagag cacagcgaac tccggagcgg gcgcgaactg ttcccagtac
    18721 gccttggccc ccagctgatg caggtgagtc cggatatggc gggcgtgctc ccgcagcttt
    18781 tgattccggg tctgctcgtc cggggcctgg agggcttcca ggtaggcctt gaggggagcc
    18841 ttggaatcca ctacgatgtt tctccggttg ggaagcctga cgatcaagtc gggtcgaagt
    18901 cggccctgct cccccgcaac ggattcctgc cgaacaaaat cgcagtattc gaccatcccg
    18961 gccatctcga ccacgcgtcg cagttggatt tccccccact gcccccgcac cgtcggggtt
    19021 ctcagggcct ggacgagctg cgccgtctcc ttctggaggc ggctctggac ctgcgccagg
    19081 gctctgacct gttcgtgaag gcttacgtag gcttccgttc gacttcgttc cacctctttg
    19141 atttgccctt caaccttgtc cagggaactc tcgatgggtt tgatcagctc ctgaatggcc
    19201 ttttgccgtg tctccaggtc gttcctggca ccctggtgaa accgctcgaa ggtggatttc
    19261 gccagctcca ggaaagactg gttgttgctc ttgagggctt ccgccgaaag cgccctgaat
    19321 gcgtcggaga gatgcgtctg cgcctcccgg atcaggccca gcttttcctg ggagctggtg
    19381 cgctcgagat gaaggcgggt ttcgagctcc gagatccggg aacggagctc gctgttctcc
    19441 gaccggaccc tcccgaggaa ttcttccttt tcctttagga gggattccaa cggttcgacc
    19501 ctgaaacctc tctcctccgc tgcggcctgt ctttgaaggg ccgattggag ttcttcccgg
    19561 agccgccttt cccggatcgc ttgttggtcg agggcttcct tggccgcccg aagggcctct
    19621 tcccgggccg ccattcgttc ggtaagagcg gaccgctcga tctcgatccg ggtttcctgt
    19681 ccggacagca cggacatcca tcgccatcgc aggaaaatcc atgccagaac ccctcccgcc
    19741 acgatcccgg cggaaaagaa aagggcgggt tccggaagaa aggcccatcc attcatgccc
    19801 acgatcctcg gtgagggaaa tccagtcaag gtagctgccg cggccgaaac atgccttccc
    19861 ggcactagta ctgccacagc ctcgcccttg tcaatggccg gaacggattg ggcggacgcg
    19921 gaagcgtgcc gccgggcaga cggccgaggc ccgttccttg accggggtgc gggtttccac
    19981 tatcttgttt tggacattct atggcgcaaa ggagaaaacc catgacggaa tgccgaattc
    20041 ccgatcaacc ggttacggat caggccgtgg cggacatcct gaagcggtcc cgcaccattg
    20101 cggtggtggg gatttcccac aaagaagaac gtgacagcca tcgggtggcc aagtacttga
    20161 aagaacacgg ctaccgggtg atcccggtga accccaagta caaggaagtg ctgggagaga
    20221 aatgctatcc cgaccttccg tccgtgcccg agcacatcga cgtggtggac atcttccgaa
    20281 acgtggaggc catccccggg atcgtggacg aagcgatccg gatcaaggcc ggcaccgtgt
    20341 ggatgcaatt ggggctggct caccatgaag cggcgcaaaa ggcccgctgt gccggggtgg
    20401 gcgtggtgat gaacaagtgc atgaagatcg agcactcccg gctcctgacc tcgtagcgtc
    20461 cggcgggtcg gtctgcggcg cagagagggg cgcctctatc gaagccgtcg cccaaggatt
    20521 gtctcccgca ccctctcccg gaaggggacg atgtctccca gaacgccatt cccccctgcg
    20581 gtggaaaccc gcccgcccct agggtccatc gggtcggtga tccggtatta tcgcaccctg
    20641 cacgggacct gggacgacga ggccgggggc ccgtatcgat tgacctccct gggaacatgg
    20701 gcggcatccc gggtccccca tgtgtatcat ttcttcaaaa aaatccggtt ggatcgcttc
    20761 cccctgttcc tggacctggg aagcggggac ggcctcgtga cttgcgttgc cggacttttc
    20821 acccgcgccg tcgggatcga ggtggatccg gttctttgcc gaacgggcct gcaggcagct
    20881 cgggcactgg ggttgaccgg acgggtcgac tttgcctgcg ccgactaccg aagtctgcaa
    20941 atccgccggg cggatgtttt gtacatctat cccgacaaac cgatcctgcc gttccgtgaa
    21001 ctgctcgagg attggaacgg aaccctgctg gtgtacggtc cccattttcc ccccgaaggg
    21061 ttcgaagtcg tggaagactt gcgatgcgcc agggagcggc tccgcgtcta ccgcaactcc
    21121 cggacccgtt gaccccgcac aaagggcaag ggcccccggg cggcctcccg ggagccgacg
    21181 gccctcagcg aacgggcaag ctgcctgggg cggcgtgtgg tggacgggca gggtcccggg
    21241 tgtgcggagg ggagagcttt cagtctctct gtttcctggg ggaagggggt cttcgtccgt
    21301 cggaaggggg ttcgtccttt gcggcttggg attccgacgc gtcgcgcctg gctcccttgg
    21361 cttgggggcc cttcttgtcc ttctgtttgc gaggcgatac cagggccggc gtctgctgtt
    21421 cgcggccctt cttttcagcg gaaatcggca acttccgggc cttgtcccgg ggcgaggtga
    21481 tggacttcgg ggccgtgtcc ctgaaggagg acacccttcc cttctcttca tccgcaccgg
    21541 gtcgcttgga tgccggacct ttctgcactg cgggctccgg gatcgtgctc ccgacggatc
    21601 cccgggggct cgcttcccgc aatcccgagg gtgaagcgga ggttcccggc tccgggggag
    21661 gagttgacgg ggagggtccc cggctcgaag aaggcggagc ggaacccgcg gcggggacct
    21721 gggaaggggc gggcctgacc cggatggaag acagcacgtt cccggtcccg tcgaaatagg
    21781 tgcggtttcc ctggtcgtca tccacgtaga cgctgccggg aggggctgcg gggagcccgt
    21841 aaacgggggt gggaggaatc ggcgtaacgt ccgggcaggg ggggaggttg ggaacggctg
    21901 ccgccctctt ccggacctcc tccatggttt ttcggatctc cccggcggga cccccctccg
    21961 actcgcgtgc ggtctcaaac gcacggattt cttccagggt gacgttcacc tgcctgcgct
    22021 tttcatggat atcgaagcaa accgaccggc aaattggaga attctggaaa gccccggatg
    22081 tcttgaagca aaactggagg atccccatct tgctgtggat tgcctcgagg gtggtgtccg
    22141 cctcctgcat cttcgccgag gtcagggtct cccgggcgga gatttcgggt tcggggggca
    22201 cgacggtttc ggggatcatc ttctccgacg gtccccgaag tggtgcggcg gtttcccggg
    22261 gtgccggaac ctccggaacg ggggaaggag gctggacgct ctcccggggg ggagggatca
    22321 cccggggctg ccgggaccac tggagggagt aggcggcggc ccccagcaag atgaggagcc
    22381 ccgccgccag gaccggcttt cttttgatga aaacggaagt cgagggcgtc gttgtgcggg
    22441 cttccgcggc aacaccccgt ggaaaataga tgaaatccgg gtccccgtac agcacgtagg
    22501 aagcccacgt gaggttctct tctccataac ggctgattgc ctggagacga gcctccctca
    22561 tggcttcccc cacggattgg gaccggatga gcctgctgta aaaggtttcc acaaaagcgg
    22621 cggtggactg ctccgccagg tccgcggcgg cccccacata atgccttgtc ccggcccgca
    22681 caaagccggg agccagcccg gtgaaacgaa ggccggggga gccctgggcg ctgcgccccg
    22741 atggacaagc attgacgaag accagggaag gcaggggctt cttctgtgcc atggacgaaa
    22801 gccaacccgc ctcgaatctt ccgtcggcca ggagaagacc gttccgggac gggtcggcga
    22861 cgtcgtactg ggtgtggcct gcaaagtgga acaggtcgta ctcgtagagg ttggatcgaa
    22921 gaaacccgca agttccatcc cggcccttca ggctcaccgt caccctggat cctgcggttt
    22981 tgcccagttc tttctgcagg ccgattcctt ccgatcgaat cgtcggtagg ttgccccggg
    23041 gatctgccac gaccatcatc ttgagaggct ctttgccgga gggcctcgaa ggcagggcgg
    23101 cctcatccgg aacggccaga gatcttccca tggagtactt gaggcaccag aagccgtccc
    23161 ggccgtgaag cagttcccaa ggaatgaagg caaaggtgtc gtcgatctca accaccaggg
    23221 tttgaacggg gtggatggag aggaactcat ccagtgcccg ggggaccagg gtctcgtaga
    23281 gacttcgggc aacatccttc agcagggcga gcttctcttt ggagatccgg cctcgccgat
    23341 ccggcgagga caggaccgaa tcgattcggt ccgcaaggtc gcggatttcc ttttcccggt
    23401 agggcatttc atggaccgcg gcggccgcct ggagcgtccc gcgggtgagg gcggcatgga
    23461 ttcgggtctt ttctccctga agggtgtaga ccagccgcag gacggttccc tcgggccgga
    23521 cttccagctc ttccctccag ggggctcttg gcaaggttgt ttcctgttgc ggcttccaga
    23581 gaattcgata gacgtccaag ggggtcgatt tgcccttgaa gctcttggct cccgcataat
    23641 cgtgggcaat gccgggttct ccgccgaggg cgtcgatcac gtccttggaa accaggatgt
    23701 ctccactctg ggcggcggaa cagattctgg ctgaggtgtt gaccacgtcc ccgtagatgt
    23761 cgtgggtctc cacgattccg ggaccatagt ggatgctgat cttgatccag atttggttgg
    23821 cgggtctttt cccctcgttt tccacggcca gggccttctg catttcgatg gcggcgcggg
    23881 cgctgtccgc gggcttcacg aaggcgacca tgagaccgtc gcccagggtc ttgacgattc
    23941 ttccatggtg tctctggatg acgggtctga gaaggtcgtt gtacctctgg accatgaaac
    24001 gaccctcgat gtccccatgg agttcaaaaa actcggtaga ggcgaccacg tcggtgaaca
    24061 tgatgcagag ggtgcgctga aacttctcgc ggatggcctg gtcaatctcg gccttcttcc
    24121 ggaggaattc ctcgagggag acttcccctt ccttctcgtg gttctgggga gatttctttg
    24181 atttctcgtc ggtcattggg agtccccgaa tccacggccc gcctcgcaaa acctggatat
    24241 ctgccgggat gcaggatacc aggagtgccc ggcgaggtca accctccggg gtcgagtctt
    24301 cagggtcggc gggaggcgcc gacgacgccc acacagtagg agtgccgtcc taccttccca
    24361 ggatcgcctc ggcaacggcc tcctgctcct cctcggaaag gtagggatgc atgggcaggc
    24421 tgaagaccct gcaggcacag tcctcgctgc gcggaaaatc gccccgctca tagcccaggt
    24481 gagcgtacgc cggctgaagg tgaagggggg tgggataata cacggctgta gggatgcccg
    24541 cttccttcag ccgtccctga agctgggatc gtgtcttttg gtcccgggcc gtgaccgtat
    24601 actgagccca ggcggacaca caatcccggg gaattctcgg cgggattacc ggggaatcct
    24661 ccgcccccag aaggtcgccg tatctgcggg ccaccctctg ccggagggag agttcctcgg
    24721 gaaacacttc gaacttttcc aacaggatgg cggcctggag ggtatccagg cgaccgttca
    24781 ccccgattct cacgttctcg tacttgtggg aaccctggcc gtgtacccga acggaccgaa
    24841 gcttctcggc caggtccgcg ttgttggtga agcacatccc tccgtccccg taccccccta
    24901 gaggcttggc ggggaaaaag gaggtgcagc cgatcccagc catggcgcag gctttcttgc
    24961 cgttcttttc ggctccaaag gactgggcgg cgtcctggag gacgaaaagc ccgtgttttt
    25021 ccgccaggga gttcaggctt tcatagtcgg ccgggagacc gtagaggtcg actccaatga
    25081 ctccacgggg agtcagtttc cggtccccga ggccccgggg gagggggtag tccccgggac
    25141 ggtcccggac aagggccgtt agtgcccgtt ccacgtgctc ggcatccagg ttgaaggtga
    25201 tggggtcgat atccacaaac accggggtgg ctcggagaag agaaatgacc tccgcggtgg
    25261 ccatgaaggt gaagggggtg gtaaagatcg catcgccggg tcccacgtcg tacgccatca
    25321 gggccatgag cagggcgtcg gttcccgatg cgcaacccac ggcgtgggcc gtgcccgtgt
    25381 actgggaaag cgccttctcc aacatggcga cttccggtcc catgatgtat tggccgtgat
    25441 ccaagacttg ccggattcga tcttcaaggt tgcttcggat tctcttctgc tgcatcccca
    25501 ggtcgatgaa ttgcatggcg gtcttcctcg tgaagtgggt gaaatgcccc ggcgttccgc
    25561 tgccctgggg agtccggatg gactcgaggc cggtggcgga agtatgggca acttgcggtt
    25621 tccgaaatcc ttccttgaac gaaacaacag tcccgctttc cgggtggaac gtcccggggt
    25681 ccgggggcat cgtggggtcg ccgcggtccc ccccggggac catggttgtg aactcaaaag
    25741 acttgtgata atgagggctt tggaaggcat ccgctccatc ccgtcagggt tcgggcccac
    25801 ggtgcgagaa cagaaatcaa ccaagatcct gcggtgctcc ccgtgggtcg aggccgtgcg
    25861 tttccgtctc cccgggtgaa tccatcccgg gggttccccc gtcaggacct taaccgggct
    25921 tgatccttgc gggtgtgagg tccggacccc tgtggcaatg gcacagtttg gggtggatgt
    25981 caactgccga cgcggttttc aacggccccc ccggggggtc cgggtctcga aggaatcgaa
    26041 aaatgaccgc taccgcccat tctcctgaaa ccaagtacgt gtgcattcat ggccacttct
    26101 accagccgcc ccgggagaat ccctggctcg aggcggtgga gagggaagag acggcggcgc
    26161 cctctcacga ttggaatgaa cgaatccaca tggagtgcta tcgcgccaac acggcggccc
    26221 gcctggtgga cgagaagaat cgagtgctgc ggcttcggaa caactaccag tccctgagct
    26281 tcaattttgg tccgactctc ctacgctgga tggaagggca cgatccggag tcgtaccgga
    26341 ggatcattca ggcggaccgg gaaagtcacg gtcgctcggg ccacgggaac gctatggcac
    26401 aggcctataa ccacatgatt cttcccctgg cctgccttcg cgaccaggtg acccaggtcc
    26461 ggtgggggat ccgggatttc gagcaccggt ttggcaggaa gccggaggga atgtggctgc
    26521 ccgagacggc ggtcagccgg gaaaccctgg gggtgctggc ccaggaggga atccggttca
    26581 ccatcctgtc cccctaccag gcctcccaat ggcggttgct tcacgagacc gcttggcggg
    26641 acgcacggga cggtaccatc cccaccggcc gggcttatcg gttcgattgc ggtggggggc
    26701 gggtgatcca tctcttcttc tatgatgcgg aactggcccg gggaatcgcc ttcgagcggc
    26761 tattggaaca cagcagtcgg ctcctggacc ggattcggca cggtttcgat catcgcgtct
    26821 gcgatgccgg ggagccctgg cttgtccact cggccaccga cggagaatcc tacgggcacc
    26881 atttcaaatt cggagacatg gccctggcgg cggccttcca ggaactcgac cgtgacccct
    26941 catatcaggt aatcaactac ggggaattcc tggcccggtt tcccacgacg gcggaggcgg
    27001 acatcgtgga aaatacggca tggagttgcg ctcacggcct gggcaggtgg gagagagact
    27061 gcggatgccg aatcgggggc gaccccggcc tgcaccagaa atggcgggcc cccctccgga
    27121 aagccctgaa ccggcttcgc gacgccctgg cactccatta tgagaaggag atgggtcggt
    27181 tgtgtctcga cccgtgggag gccaggaacg actacatcga ggtggttctg gatcccgagg
    27241 cggggaggga agatttcctg agtcgacatg cccgggcagg cccggcaaac tcggatcttc
    27301 tccgtttctt ccaactcctc gagatgcagc gctgttccat gttgatgttc acgtcctgcg
    27361 ggtggttctt cgatgacatc agcgggctgg aaagcgtgat actcctcaag tatgcagccc
    27421 gcgccatgca actggcccga atgaccggct ctgctcccct ggaggactct ttcctccaat
    27481 tgctggaaaa ggctcccggc aatcgaaagg aattcgcgaa cggggccgag gtttaccgga
    27541 agaaggtatt gcccgaaatc gtggaattgg accgggtggt ggccaaccat gccgtacgtt
    27601 cccaggctag gacccgcggc cccggctatc gcctgtattg ctggcaaatt cttccccaga
    27661 aagaagagga cctgggttcc tatcccgccc ggagcctgta cgggcatgtg cgggttcggg
    27721 acacacgcac ctgggaggaa gccgactttc tgtatgcagc ctttcatttc gggggcctgg
    27781 atttccgatg ctccgtaaaa ccctacggag atgacgccga ataccgggca atcctggctt
    27841 ctctccagcg atccatggaa gaacagaaca tggtcaacac cgttcgattc ctggatcaga
    27901 ctttcgggac ggcattcttc ggtctgcagg acgccttccg ggacctgcgc tcttccgtgg
    27961 ccgtcgaaat cagccgggaa aagctcaggt tgtaccggga cctccagcac catttctacc
    28021 agactcatcg gcccctgatg atttccttgc gccagtgggg actgcccctg cccagtgatg
    28081 ttcgaggggc cgtccgcagc gttctgagcg acgaagtgaa ccacatcgtg cgcgagatcc
    28141 tggaacatca acagggctgc gaagcggaag gcgaatgctg ggaggacacg gatttccact
    28201 accgcgttca cctgggccgt ctgaatgcga ttcttgagga tgcccggtgg tgggggttgg
    28261 tgctgcgcac cggcgacctc catgatgtcc tgggaaaggc catggtggag tgcttgtccg
    28321 cattgggcaa gggcttccgg gtcgaggatg cggggaggct cgcacggctg ctggcggtat
    28381 gtgaacggtt gggcgtcaag cccgaaacct ggaagctgca aaccctgttt ttcgagatga
    28441 tcgctcaatc cttgaaacag cccgaggaat actggaataa gttgaagttc cgagggtttg
    28501 tggaacgcct ggacgattcc ctgggctgca actttgcccg gttggctccc ccttcgccgg
    28561 ggccttcccc gcgaagcccg ttggcaacct gagtgcggag tggtaaatag agaacgttgg
    28621 gtgcggccgg tcggccggtg ggttcgttaa gatcgtgagt cgggccggga ggccgggttt
    28681 tcccggcgag gggggacgga ttccccgggc cttggggaga tcgacgacaa cagtcaggaa
    28741 gggacgggag acatgtgtga agccaatgcc tatttgatca agaacggtcg ggaagagctc
    28801 gtcatggaag cggtggacgt ggtggagagt gagggggagg tccttcgcgt ggccaacata
    28861 ttcggggagc agaagatttt gaaggccagg atccggagca tgtccttggt gaaccacaag
    28921 atcgttctgg aggaataggg caaggttcct ccaatacggg cggccccacc cggtcattct
    28981 tctctttccg agggtcccga agggagccga ggaagccgct gctcacagta ccaatccatt
    29041 tgcctgcaaa tcccccgaag catcctcacc tcccgcgcgc gcagctcgat cctgctaaag
    29101 aatcttcgga ttcccgtcat ccagtattcg gggttttccg ggttgatgaa atggatcctt
    29161 gcgagaatgt cctggagctg ggcgtagagg gcttccattt cgatcttctc ggccaggcgg
    29221 ggaacgaagc ttttgggttc gtcgaggctt gcgagaaaga tctcgtaggc cagtaccatg
    29281 acggcctggg ccaggttgag ggaggcgaac tccgccgtgg gaatggtgac caatgcgtcg
    29341 caataccgca ggtcccggtt ggtcaggcct cgatcttcgg ggccgaagag cagggccgtc
    29401 cggttcgacc ggctgagggt caccagctcc tcggccaaac gccgggggct tttgaccgcc
    29461 tggcggtggg acccttttcg agccgtggtc cccaccacgt actggtagcc cgccagggcg
    29521 tcttccagtt cgtcgtgcac ctccatggcc tcgatcactt cttgagcgtt gtgggtggcc
    29581 attttcagaa tccgtgtaag atcgcaatcc tcgggccgaa cgacggcaag tcggccaatc
    29641 cccatgttct tggcggcccg ggcggccgct ccgatgtttt ccgggtaacg gggcctatga
    29701 aggatgattc caatattctc gagtttgact ttcatgcaca atggatcccg cggattggcg
    29761 accggccgcc ccgggataag gaccggggca cggccgcaag cattataggg tttcaaagga
    29821 acaaatcaag aacggcggaa cagaacgggg gaatccgaga tgccgattgt ggggatctgt
    29881 ccgcggatag ggacacttct ctttcctgcc cgggcaaatg gagcgatttt cctccgctga
    29941 tgaaagaggc cgggagaggg tgaacggccc gtttgtggag gggccctcta tgccgagcgg
    30001 cccgcccacc gggaggaatt cgcagttgag gattgactcg gtggctccat gggtataaat
    30061 acccaagaat acagatagaa tcaatggttg ggaccccgat cgatccgact tcctccccaa
    30121 ggagggatcc atgaggcagc agaagatccg agaatcggtc atcgccggaa gctggtatcc
    30181 cgggaacccg gagaccctgc ggcaggagat cacccggtat gtaaacgagg tcccggatcc
    30241 cagggtcgaa ggaacgctgt tgggcttgat ttgcccccac gccggataca tgtactcggg
    30301 gggcgtggcg gcccatgcct ataaactgct gaccgggcac ccgttcgatc gggttctcat
    30361 tgtggcgccc agccatcgag cccatttccc gggggccagc ctgtcccggg tgggcgggta
    30421 tcggacgccc ctgggagtcg tccccctgga cgacgaactg acggagctcc tcggcgctca
    30481 ttcctccctg gtgggctatt tccccaatgc cgattcccag gagcattccc tggagatcca
    30541 gcttccgttc ctccaggtga tgctcaagga ttttcgtctg acccccgtcc tcatggggga
    30601 gcaatccccg gatttttgcc gaaagctcgc cgacgtcctt gccgacgcgt gccgggacaa
    30661 gaacgtgctg ctggtggcca gcacggacct ttcccattac cattcccagg gtacggccag
    30721 gcgcctggac caggtagtcc tggaccgggt gggggccttg gaccccgagg ggctctccga
    30781 ggacctggag caagggcgat gcgaggcctg cggcggcggc cccatggtga cggtcatgct
    30841 ggccgccaag aaactgggcg ccgtgaatgc cagggtcctc caatatgcca cctcgggaga
    30901 cgtctcgggg gatacccgcc aggtggtggg ctacttgtcc gcagcgattt tcggccctcc
    30961 gccccgtgcc gccgaaacct cgagggaggg aagagggaga gtcggagtgg acttggggtt
    31021 gagccccgag gaaaaggacg ctctgcgcag aatcgcctat gaagccattc ggagccgctg
    31081 tctgggaacg cagatgcctg agatcccggt ggacttgccc aaactcgaag aaaagagagg
    31141 ggcctttgtg tgcctccata aggacgggag cctcaggggc tgcatcggga tgatcgaagg
    31201 aaaggagccc ctgcatcgaa cggtccaaaa catggccgtt caggcggctt ttgccgaccc
    31261 gaggttctgc gccctggccc cggaggaact ggagaagatc gacctggaaa tttcggttct
    31321 aacccccctc gagcgaatcc aagatccgtc ggaagtgaaa atcggaaaac acgggttgtt
    31381 catacgaaaa ggatatcatt ccggtttact gctgccccag gtggcaacgg aacacgggtg
    31441 gggagccgtt gagttcctgg aatggacctg ccggaaggcc gggctccccc cccatgcctg
    31501 gaaggccgag gatgcggaga tctatgtctt ctccgccgat attttttaga aatcgtctcg
    31561 atcccggggg agcttcttcc acgtcgttcg aggggacccg ggatcgaatc cggcccgagg
    31621 gccggattcc gggagatgcg ctcaggtccg ggttttgacc ggcgggatgg acgtgacgga
    31681 ctcccgaggg gcgaaccggc atttgcggta gaggggagtg gaccccgttc ttgctttcat
    31741 gacttgcgag ggtgatttgt gggtattgtg ctggattttc aaaaagggcg acgtcatctg
    31801 ctggccaaga gggccttccg gaactggggc cgccgcttca cggatcaatt cgacgaggac
    31861 accagccttt cggctctcag cgacgggacc ctggggcttc tgattcgcgg cggagaggaa
    31921 aacctgcttc cgatctacga cctcatcctg agtgtgctgg ggatgggaag cggagcccgt
    31981 ttctatgacc tggaaggatc ggaaaagatg tacgtcatgg acatctccat cttcctgctg
    32041 gaccagttgc ggttcgaatc catgaaaagg ctgggctggg tggaagactt tcccacccgt
    32101 cacataagcc tcctggacct ggtggaacag ttcggagaac acttctcctc ccggcgccac
    32161 gaaaccccct ttctctctcc cagccacgcc ctgtacggcg aatacgagaa gaccttcgag
    32221 ggggatcgaa gcctgtttgt gcgaagattg atcccggagg ctcttcaggc ctacatccgg
    32281 gagagtgaga aatcttaggg atcggcggct ttttgcgggg gatttcgggg tagggcatgc
    32341 ccaaagagcg ttttcacctt ctcctggccg aggaaaccct caggcacctg gagcgatccg
    32401 gcagagttcg ccgcttctcc gtgcaacaga ggcacgggtt tcttttggga gcagttcttc
    32461 ccgacgttct cttttacgac cttccctttt tttcccttgc gggatcgggc agggagcttc
    32521 atcgacttca gaagccccac ggcctcgagt ttttccggca atggtcggaa aaggcgaagg
    32581 ggcgatactc cccggccgcc cttgcttgtg tgctcggagt ggcctgtcat tttctcgccg
    32641 acgggtactg gcacggattc atcggaaagc tgtgcgcgga tcccccgagg gtgggcaacg
    32701 gctttttggg gcatacggag aaatgccgtc acctctggtt ggagagcgag ctcgaaagct
    32761 actggatggc gaagctggga cccccggacg ggtatttccc cgtgttgagc gggtttcgaa
    32821 gggggaccgg cgcgaatcga acctgcctca ccctgttcaa ggatttgctg gacgaggcgg
    32881 gaacggagtc cgtgcccacg gagtcccgga ttgcccggtg cctggtgttt cagaccatgc
    32941 ttttgcgcca gtttgcccag ccccggtggg agcgatggag atcccgcctg ctgtccgcac
    33001 ggtcgactcg gcgattgggg gttctcctgg tgccgccccg gcccaccctc agggagccgg
    33061 aatcttggga accgcaaagg gaaggggacc ccggggccct gtgtacttgg gacttcatgg
    33121 gccgctcggt gtctttctta tccaaccgtc tgagtatgct tttaccaccg tcttgacgat
    33181 ccccggggca tccagatctt cctgccggta caagtcttcc ggctttccgg aaagtccgta
    33241 gttcctgacc cccagtcgca ccaatcgaca ggaagccgcc tcgtctgcca ggatgagggc
    33301 cactcgcgca cccagcccgg tgtcgatgtg gtgatcctcg acggtcagga ggaaccccgt
    33361 ggcggcggcc cgaagaacgg cttccctgtc catggggcgc agggaagcca tgttcagaac
    33421 ggccaggtga atcccgtgcc gggatttgag ctcgtcgccc gcccggaggg cgtggtggag
    33481 gaccgttccg tagctcagga tggcgccctg gcttccctct ctgagccaat cggcttttcc
    33541 ggggacaaag gtgtagttgc ccgcaaatgc cggggctccc gcttggtcca ggatgacggg
    33601 aagcttggat cgccccatgc ccacgaagaa gttccccggg tgggcggcga tataacggat
    33661 gatgcggtca gtctggttgg ggtcccccgg catgaagatg gaaaaaccat agaggttttc
    33721 caggagcccg atataatcaa tgcactggtg ggtttggccg tcttctccca catcgagtcc
    33781 cacatgggtg cagacgagct tgatctgggt gctgttgatg tcgtttagcc gctgctgatt
    33841 ataggtctcg cagactccaa agactccaaa ggtgctgaag aaggaggcaa agccctcctt
    33901 gctgagcgct ccggccaggg tagccgcgtg atgctcctgg attcccgctt cgaagaaggc
    33961 gttgggggac agccgatgga aggcgtccag tttcacggag ccctccaggt cgcagctcac
    34021 ggccaggacc cttgggatgt ctccgtcgtt gttccatcgg gccaaatctt ccatggcggc
    34081 gccgtaggcg gaccggttgt cggtaacggt ccccggagca taggttctgg gctcgccccc
    34141 ccggacctcg gggacaaccc ggggaaagca gaaatggggg cgaaaatcga agaaggtctt
    34201 cctcctgctt cggaactcgg ggatgggatt ctcgaggccc agttccgcca gggcctcccc
    34261 ggccagggtg tcgtcgaggg ccgagccgtg gtacttggcc ttgttttcca tgaacgagac
    34321 ccctttgccc atgatcgtgc gggccacgag aacggtggga tatcgtgcat cgtccacttc
    34381 ctgggcatgg attctcttga gggcctgaaa gacggcgtcg aaatcgtgcc cgtcttcgag
    34441 gtagatctgg ttccactggg cggccacgta gtcggctcgt atggcctgag gcatgaccag
    34501 gttggtgttt cctccgattt ggaggtgatt tctgtccacg atcccgaaca gattgttgag
    34561 ctcatatttt accgcgaacc ggcgggcttc ggagatctgg cccttttgct gctccccgtc
    34621 gcccatgagg acggccactc ggcttttctt tttgcgaagc ttcaaggcca gggccattcc
    34681 cacccccgcc gagagtccct gtcccaggtt ccccgtgttc cattcgattc cgggaacaca
    34741 gcattccacg tgcccggcaa aacaggaccc cgcgcgccga aattctagga agagctcctc
    34801 ctcccggaca tacccgaact cgcacaggac actgtaaacc cccggactga tgtggcccat
    34861 gctcaccagc aaccggtccc ggtccggcca ggcgggctct tcgggccgat gctgcagggt
    34921 cgagaacagc atcagcaaca agtggagaga cgacatggat ccgccgggat ggccgctccc
    34981 ggccagggtc gtgctcacaa tgatgcgccg catgcatcgc agccacatct cctgaagggt
    35041 ttgcctttgt gcttcggtca gagcggcctg tcgcaaagat agggtgaaca tggggactct
    35101 ctgtgccttt ctagactcaa tggacggctt tgggatcagc tgccgcagcg ggcttcatct
    35161 cgctcaagcg ttggaggacc cgctccgtaa agtccaacac cgcttttttg aagggggtct
    35221 ccccggggtc ctccaacaca gggcggcctc gatctccccc ggccaccacc gcaggatcga
    35281 atgggagggt ccccaggaag gggaccttca tctcttgaga ggttttcagc ccgccgcccg
    35341 tctgaaagat gggaacaaat ccccggcaat ggggacagaa caggccggac atgttttcca
    35401 ccagccccag gatctcgagg tttaccttcc ggcagaaatt gatggatttc ctcacgtccg
    35461 ccagggcgac ctcttggggc gtggtgacga tgacggccct cgcatcggga atgatgcggg
    35521 ccacgctcag gggctcatcc cccgtccccg gggggctgtc gatgaccagg gcgtccaggt
    35581 cctcccagcg gcagtcggcc aggaattgct gaatcacccc gtgtttcagg ggacctcgcc
    35641 agatcacggc ggaatccgta tcttcgagca tggattcgat ggagaccacc ttcaggttgg
    35701 gtcgatattc atgggggagg atctgctggt cctgggtgat gttgagaatc ccatggagtc
    35761 ccagcatctt cgggatgctg ggaccgtgca ggtccacatc cagaaggccc acccgcattc
    35821 ctttgttgga gagagccagg gcgagatagg cggcaacgct gctcttcccg actccccctt
    35881 ttccgctcat gacgagcagc ttgtgttgaa tccgtgccag tttgcaacga gccaagtcct
    35941 gtttggacat cttgtccttg ctggggcacg tatcgcagga tttcggatca caagaactca
    36001 tgcattctac tccctgtctg tccaatgaat tccgtcgaag gggaaaaagt gggggctaca
    36061 ttaaaagcat tccgccctct tgtcaacttg gtgccggcga ccggcttttg cgccctcgag
    36121 gatccggggc accgggaccg agaagagggc atgctcctgt aagctaagca cggatggcgg
    36181 aaacggcttt cactttgggg tcctcccggc agaaatcttc caagattccc cgggaagggg
    36241 cggcgaggga atccgtgccg gggctggggc cgaggacctc cgcggaggtt cccgcgttcc
    36301 gtcttgaaac ctgcggggaa aaaagtcatg atagacggga cccaagttcg ttcgagtcct
    36361 gcggacgagc ccccttcaag gatggagacg aaacgccatg cccatttacg aataccgttg
    36421 taagaggtgc aagaacggat ttgaagccct cgtccggtcc gcccgggagg aatccctggt
    36481 cgagtgtccg cattgtgaac aaggagagat cgaacgggtg ttgtcctcct tttcggtgac
    36541 ctcttccggg ggtacttcct cgagtggggc aagctgccgg cccaaaccgg ggggattccg
    36601 ttgagtgtga gtgtcaatcc ggcaggttgc cggatccagg gttcaacggg cggggggagg
    36661 ttcccgaacc caactttcgt gcgtgaggtg caacgatgac cttgtatgtg gtgctgtttg
    36721 tgagcgtgat tgtgggctgg ttcgtctgtc agcgatggat tctgcccaaa ctgggaattc
    36781 ccacctgact ctcccctcga tgtgagatcg gagatccaca agagaagaag aatccccgtg
    36841 cgcaatccaa gaaacccaaa ggccgataag gctttccttg aagccccgtc ctcagcggga
    36901 agcgggagat cggggccgtt tgggaccgga ggagcgtcgg cttgcgacga ataggagcgc
    36961 aaggccgaac aaggagagcc ccgccaggac ccccgtgacg accttccaat ccaggctggg
    37021 gaacagcagg atgctctgcc acgccctctt gaaccacccg gccaggggga tatcctcccg
    37081 gcttgccagg gggaccgtcc tgctgggttg atcccccacg tagaagacca gctcgcccag
    37141 ttgctgattg gcccgcacgg gggcggtcac ctccttgggt gcctgcactt cccagcggag
    37201 gctgttcttt tgtgcctggg caatgaggaa gcccgccggg ccctggggat agaggtccaa
    37261 ctggtccttc aaccctttcc acaccttgag ggcggcgacg ggctttcctt ccgggaaagg
    37321 ctggatgagc acaaattggc gaaatccgta attgagtagc cttagggctt cccgttcccg
    37381 aattcccggg gtggtcgctc ccatgaccac ggccagaagt ctcgtaccgt cccttttggc
    37441 cgtcgcggcc agatgatagc ctgctgcggc cacgtacccg gtcttgaggc cgtcgacgga
    37501 cgggtccttg agcaggaggt ggttccggtt gtattggacg atattgttgt aggtgaactc
    37561 acgcatggaa tggtagtgca gggattcggg aaaccgctgc aggtaggaca gacacaggcc
    37621 ggccatgtct cgggcggtgg ttacctggcc ttcggcgggc aggccgtgag ggttcagaaa
    37681 ccggcttcgg gtcatcccca gttcccgtgc cttccgattc atggcgtgta cgaagccctc
    37741 cacgctcccg taaagatgct cggccacggc cacacatgca tcgtttcccg acacgacggt
    37801 gattcccttg atgagttcct cgaggggaat tctggacccc acacccacga acattttgga
    37861 cccccccgta cgccaggccg tttcactgac gtaaatctcg tcggacatct tcaccagccc
    37921 ctggtgaatg gcttcgaaca ccaggtagag ggtcaaaacc ttggtaaagg atgcgggttc
    37981 gatgatgagg tcgggttcct gttccaagag ggttgccccg gtggagacct ccacgagaac
    38041 ggcggaccgg gtattgatct gtcctgcggc ggggtctccg gcgggcttgc ccgggtgagc
    38101 cgttcccggg gacctcttgg agcgggctcg ctccacggcc ccggttccgg ccggcagcgc
    38161 cagggtcaag gagagaaaaa acgtcaggaa ggcaatcaga tttctttttc ggaacatgac
    38221 caacctttca aaactcggtg aggggaatct cgaggaaaaa actcccgtct aaagagccac
    38281 cacgcggttt ctgccggcgt tctttgcctc atacacggcc cgatccgccc gattgaaaac
    38341 ggcttcctcg gtgtggtccg agggggtcgc ctgggttacc ccgacactga tggtgattcg
    38401 gatttccatc ccctgataca cgaaccgggt gttttgcacc atcttgcaga gcctttcggc
    38461 gaccgcgcag gcggcttccc gatcgattcc cggcaacagg atcacgaatt cctccccccc
    38521 gtatcgggcc agaaaatccg tgtcccggag gatcggccgg attctcttga tcacttcctt
    38581 gaggcaacgg tcgcccgcac gatgcccgta ttcgtcgttc actcgcttga agtgatcgat
    38641 atcgaagaga agcatggaaa acacttgccg gtaccgatgg taacgattca gctcttcctt
    38701 cagacgctgt ttgtaggccc gctgattgtg ggctcccgtc agcgggtccg tgagggtctc
    38761 ctgctccagg gacttggttt tttcctggat ttggtcgatc tccgccttca tcgcccggag
    38821 cgtcttgttc aactcctcca tttccctctt ggccttttcc atgcggatgt cgtcatccct
    38881 ctgcttgtgt tccatggcgg ttcggattgt ggtcagcttg ttgaccacca cctccctgaa
    38941 ctccgacagg gacttactga gctgggccga gttgtggatt tcgttcatct gggtttcgag
    39001 gacgagatcg aacctgcggt tggcctggtg agcctcggac gtatgggaac aggaggacag
    39061 gaaccgtgcc tccatctgga gaagagagct tccaatgtcc cggatgaagg cggcggcctg
    39121 tttccgctct tcccgtatca gccccgtgta ggtctgaatc agggacgtga gatcggcgag
    39181 gagacccacc agatggtcga aactttcggc ttcgttcacc ttctgtcgga tttccgccag
    39241 ccgctcgagg gatgaatccc ccagctcctc gcccagttga cgcagcactt cccggtagta
    39301 gtcctgcagg tccttgaaat gaacctcccg ggaaatctcc actggttttg ccgaggggtc
    39361 ccggcgcttc atccatcgac cccagagacc gccatccccc gaccggggcc gttccgagag
    39421 gtggggttcc tgaatcaccg actgtttgag ggtcccgagg ccggcttcca attcttcgat
    39481 ctcggccccc gcaagcgaaa gtcttttgag ctcctccagg gatttcttca tggaggcgtt
    39541 ttccccgggt ctgaccagtc cggcgagggt cacgacggtg cgcctgagga aatcggccaa
    39601 tctcccggcc cggcgttcca ggtcgtcgac ctgccggacg gcctggtccc gttgctcttg
    39661 cagcaggccg atcctggcgt gaagatcttt gccttcggga ggatccgcct ggggggggcc
    39721 gcccggtagg ccgaaatcct cggatgaggg aagggagccg atcatatcca ccagttccgc
    39781 acgttccgtt aaggccttgt ggagggattg gatcgtcaag ggctgacgct cggcggagag
    39841 cttcttcagc gtctccagga tatgctccga aagccgctcg aaaaactcca gctgtttggt
    39901 ttgggataca gccacaaggt tcgctttcct gctgttggtt ttatctccgt gggggattcc
    39961 ttggccttct gcctgctccc ggttctttgg gttcaaacag ccggcactcc aggccggagg
    40021 acgaatggac caccacgcag gggtgttggc ggcaacggat tccccacgct ttgcattcgt
    40081 agggagtttc cttgacccac gtgacggcga agaatcgaca ctggaaacaa cgaatcatct
    40141 tgtaaaatca tggatctttc aggggtatga gtcccaaaaa agcaacggtg aaagtctatc
    40201 accatggaag agggaccaca agagaaacct cccccgggga cccgccgggt ttgagtggcg
    40261 aacgttccct gggagtgaga tcttctcgat ttcatcgaag atggagccgg aaccggaaaa
    40321 aaccaagaaa gtcgaaattc ttgcgcggat gaacgaaaag cctgcggcgg gggacttcaa
    40381 gttgacaggt gagcagaaaa acagtagttc tgcctgaaag cagtggagat ggttgttgtg
    40441 ggatagggga ttctctgaaa ggtgaggtat gttcattttg gcgaatttac tgtttgcgct
    40501 ggcaaaaatc ttgggcctgg cactgaacat ttacatgtgg atcatcatcg ctcgggcgat
    40561 tctctcctgg gtgaatccgg acccgtacaa tcccatcgtc cgttttctct ataacgccac
    40621 ggaaccggta ttgtacgcca ttcggaagcg agtccccctt ttcttcggag ggatcgactt
    40681 taccccgatg gtggtgattc tcatcctgat tttcctcgat aatttcctgg tcgcgtcgct
    40741 gggagggctc gccgtccgcc tgagcatgta atccattgag ggtggaaaga gatggaatgg
    40801 acttctgcgg atatacaaca caagcagttc cgtacacgct ggaaggggct ggacccccag
    40861 gaagtgcagc gattcctcga tgagatcgct caagagatcc agcgtcttct gggggaaaac
    40921 tccggcctga agaaggatgt gcagaggctg gaaaaggagc tccaggaata caaggaccgg
    40981 gagaaaacca tccgcaacgt cctgatgaac gcccacaaga cggcggaaca gatgaaggcc
    41041 aacgcggaga aggaggccaa ggtcctcata gccgaggcgg agctcaaggc ggaaaggatc
    41101 ctccaggacg cgcaccaacg cctgggtcaa ctccacgaag acatagccga actgcgacgg
    41161 cagcgggtcc tcctggaaac gaagctgcgc tccaccgtcg agacctacgc acggctcttg
    41221 gacatggaaa aagaggaaga aaaagacgac gagctgggga gcaaggtcaa ggtgctgaac
    41281 cgttgaatcc aacgcttgtg acggggtaat gaatgacgat tcaaaaagaa ctcctggaca
    41341 tcctcgcctg tcccaaatgc aaggggtcca tcactctcaa tgaaagccgg gacgggttga
    41401 tctgccgcca atgtgagctg ctctatgaga tacgagacgg gatcccgatc atgctcattg
    41461 aggaagccaa acccctggaa tcatgaaatc gcgccgccgg atcaagggac cgtgggcccc
    41521 atatcgagac ggagacagat ccgagggggc aatcccctga caacccctgc gcggagaggt
    41581 tcctctcccg ggtgcgctgt cttggggtca accgtttccg ggcttgtgcc cggttggttg
    41641 ccgacctttc gtgaccctca ggggcggggt gtgccgtgag tattcccaga gagcctccac
    41701 cggccaagct cattgtggga ctcctcttcc gggacttcga aatccgacgg gaggcattgg
    41761 cgcagctcac ggccctttac ggccccctcg atttcgcgac ccgtgcggaa cccttttcgt
    41821 atacctccta ctacgaccgg gaaatggggg aaggcattct tcgccaggtc gtcagctttc
    41881 aggatcttgc ggcggcggac gccctgccgc ggatcaagca tcagaccaac ctgatcgaga
    41941 ctcgattgtc ctcggacgcc aaacgccgga tcaacatcga tccggggttt ctcagcgaag
    42001 aacgagtggt tttggccacg ggcaagaact acacccatcg ggtctacttg cgggacggaa
    42061 tctacgcgga cctcaccctc ctttaccaaa aaggagggtt ccgggctctg ccgtggacct
    42121 atcccgatta tcgggaagcg aacctgcttc actatctggg agcccttcgc aggaaactca
    42181 ttttccagcg aaaggagaag gtgccgggag attctcaggg aggagatgct tgatttccag
    42241 catgacggcg ttcggacgaa tgcagagaga ggaagcgggc tattcggcgg ccatcgagat
    42301 ccgaagcttg aacagccgct ccctggacat tgtgttgcgt ctgcccaagg cgtacctgga
    42361 attcgaggaa gaacttcgaa agcaggtgac cgagtctttt cgccgggggc gggtggaggt
    42421 ggtggtgcag atcgaaccca cggccccgga aaggaaagcc ccccgactga gcaaggaact
    42481 cgccctgtac tactggcggc aactgcatga tctccagtcg gtattgcccg gggccgacag
    42541 gcccactctg cgggacctcc tccagatccc tcatctctac gaaaccatgg aaaccgtcta
    42601 tgacccggag gcggtgagcc gccttcttcg ggacgtgatg gcgcaatccc tggaccaggt
    42661 ccggctcatg cggacccggg aaggagaagc gctggggcgc gactgcctgg cgcgccttga
    42721 cgccctcgaa gagcagattg ggaccatcga ggtcagaaag gagccggtgc tccgggaatt
    42781 ccaggaaagg ctccttcgca gaatgacgga tcttttgggg tcgacccccc tgtccgatga
    42841 gtcccgcgtc ctccaggaag tcgcctgcct ggccgaacgg tccgacatca acgaagaaat
    42901 cgtgcgtcta aagagccaca tccaccagat gcgggtcatg ctaagggggc aggaccccgt
    42961 cgatgggagg cggctcgatt ttcttaccca ggagctgcac cgggaggcca acaccatcgg
    43021 atgcaagacc ggtgacctgg cgacggtcca ggcggtggtg cggatgaaga gcgaaatcgg
    43081 caaactgcgg gagcaggtcc aaaacatcga atgacccaac agcgttcgca gactctgtgg
    43141 cctcagcgtc ctgagtccac tccccgaaga cgttcaatca gtgagggatt ccatggaaat
    43201 aaagctgctc aacatcggat tcggaaacac cgtcatcgcg aaccgcgtgg ttgccatcgt
    43261 ctctccggct tcggctccca tgaaacgact gaaggaagat gccaagcagg ccaataagct
    43321 gatcgatgcc accatgggca ggcgaacccg ggccatcatc atcaccgaca gcgatcacat
    43381 cattctgtcg ggagtacagg ccgagaccat tgcccagaga ctcacgtcgg agtctcaccc
    43441 caaagacgcc aatctaatca agtgaggcgt gatgcgaccc taccaaaacg gatcggttcg
    43501 aggattgttg ttcatcgttt ccgccccttc gggggtggga aaaaccaccc tcatcaaacg
    43561 aattcttccc caatggcccg gcctccagta ctcggtgtcc tgcacgactc gaaagccccg
    43621 cccgggagaa gtctcgggaa tcgactatca cttcttgacc cgggatgatt tcctcagggg
    43681 gatccaggag ggtcggtttc ttgagtgggc cgaggtccac ggggagctct acggcagcca
    43741 cggggccctc gtggagacgt ggctggcctc gggcaaggac gtcctcctgg acattgacgt
    43801 ccagggagcc ggaaaggtcc tgagcagtta tccggatgcc cacacgctgt tcatcctgcc
    43861 cccgtccatg gaggcgctca gggagcggct ggaaaagcgc ggaaccgagt cccccgagcg
    43921 tcttctcaag cgccttgccg ccgcgagccg tgagatccgg gaagctccct ggtacgagtt
    43981 cgtcatcgtg aacgacgact tggaggaagc cgtgcgcgat ttcacggcgg tcatccgggc
    44041 atgccggtgc aggcgtcgcc ccatggcgcc ccgggtccgg gaattccttc agggccaatg
    44101 agggctcgaa gtgcccccgg tgccgaagct gcgcccggcg gttcgccttc ccgaggaaac
    44161 gtcccccgat gacttcccct ttcaaacgga agaaacagag tagaaaaacg caggggtccc
    44221 cccagggaga agggatgtgg atctcaggga tccatcccgt ccgtgaggtc ctcgaatccc
    44281 ggggtgcctt cgtctatgaa ctgatggttg ccagggagga cccccgggtc cgggaagtcg
    44341 ccgccctggc ggaatcccga gggattcccc ttcacagggt cgacaaacac aacctggacg
    44401 aaatcgtcgg ccattcccac caccagggaa tcgccctgca gacggaagag tacccgtatt
    44461 tgtccctgga aacgctgctg aaaccggccc aggagactcc gggatttctc ctcattttgg
    44521 acagcatcca ggatccccag aacttgggcg ctttgctccg aagtgccgcg ttcctgggag
    44581 tcgggggggt gattcttccc aagaaccggt ctgtccgggt caccggtgcg gtgattaagg
    44641 tggcggcggg agccgccgcg tgcgttccca ttgcccgggt gaccaatctt gtccgggccc
    44701 tggttctgct gaaggaagcc ggctattgga tcgtgggact ggatgccgcg ggagaagaga
    44761 cgttgtacga catcgacctg accgtgcccg tggggttggt ggtgggcaac gaggaaaaag
    44821 ggattcgccc cctggtcagg caccactgcg acctgtgtgc ccgaatcccc ggttccggaa
    44881 gactcgactc cctcaacgcc gctgctgccg gtgccgtcgc catggccgaa gtacagcgcc
    44941 agaggcggtc taggcgctcg tagacctcga tccgaacagg gcggtcccga ttcgaatcat
    45001 ggtggcgcct tcctcgacgg ccacttcgaa atcatggctc atgcccatgg acagctcggt
    45061 gagatcttcc ggggcccggg cctgccgtcg aaggtgccca agcagtctgt gcaaggtccg
    45121 gaaatagggg cgggtccgtt cggggtcctc ttcgtagggg ggcagagcca tgaggcccct
    45181 gacctgcaat ccgtccaggg cttcgacggc actgaagagc tccggcaatc tttccgccgg
    45241 aatcccgctc ttgctttcct catccccgat ctttacctgc agaagaacga ggatctttct
    45301 cgatgtcttc tgcgcctgtc ggtccagttc ccgagccagg gactcccgat ccaccgtgtg
    45361 aatccaccga caccattgga cagcggtttt tgccttgttg gactggaggt gcccgatgaa
    45421 gtgccacgta atgtccaggc cttccagggc ttccgctttt ctgcgcagct cctggacgta
    45481 attctctccc agggacagaa tcccggcctt gacgccctct cggatccgct ccgccgatac
    45541 cgtcttggtg accccgatga tccgcacgga atccggggag cgacggaccc tggcacaggc
    45601 ggctcgaacc cgttcttgga tggctgaaac attccggaac aaggggtcca tgaaacaact
    45661 cctccacaga tccgaatccg tgttcagggg accggtgctt ccgcgggaaa ccgggtggga
    45721 cgttccctcg gcacattccg gtccgatcgg cgggtcaccg cccgcctgtg gtgcgggaag
    45781 agaacaagat gggagatcgg tcaggcggcc gggacgatga ccttttgttg tgccagcagt
    45841 tccagagttt cgcacagggg cagccccaca acgttggtgt aggaaccccg aacggcccgc
    45901 accaggaagg cccccacccc ctgaatacca taggccccgg ccttatccat gggttccccg
    45961 gtccttacgt acgcccgtat ttcctcttcc tccaggcgct taaaccaaac ctcggtctca
    46021 acggaccgga tatgggcctg cccgtagccc tcatggagga ggcaaacggc gctgatcacg
    46081 cgatgccctc gattgctgag ctttctcagc gtgtcggcgg cttcccggga gtccccgggt
    46141 tttccaaaga caacgccttc caggacgaca atagtgtccg cccccaacac ccaggacgcg
    46201 ggattcagcc gggagacctc tgcggccttc cgggaagccc aggtccggac ctggctctcc
    46261 ggctcctgcc cgggcaccca cggctcgtcg caatgactgg gcttcacttc gaaccgaagt
    46321 cccacggact cgagcagttg ccttcttcgg ggggaggagg aagccaggac caaaggttcg
    46381 agggtcctgt agaccccgac cccctgcgca tctctgcccg gcggttcgac atccgccgac
    46441 ggaagggtcc ggctattgaa tggagaagac tctccgggca ttggcggtgg tttcatgagc
    46501 gatctcttcg agggtcgagt ttcgaagctc cgcgatcttc tgggcggtgt agcgaacata
    46561 ggcgggctcg ttcacctttc cgcggtaggg aaccggcgtc aagtagggtg cgtccgtctc
    46621 gaccaggagg cggtccaagg ggatgtttcg ggcgacttgc tgctgggttt cggccttgga
    46681 aaaggtgacg gttcccggga cggacaggta gaagcccagg tcgagacact tcccggcgat
    46741 gtcccaagtg ccggaaaagc agtgcattac gccgtttccc cgaagggacg ggtaatcgcc
    46801 gatcactcga aggaattcct cgtaggcgtc ccggatgtga aaaatcacgg gggacttcag
    46861 ctcgacggcc agttccaatt gttggcgcag gcatcgggtt tgactcgatc gaggttgtcg
    46921 atcccggtaa aaatccaggc cgatttcccc cagggcgacc actcgggggc cggctgccag
    46981 ggccttg
//
LOCUS       DAOOVC010000016        45476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_514_length_45476_cov_7.095793, whole genome shotgun sequence.
ACCESSION   DAOOVC010000016 DAOOVC010000000
VERSION     DAOOVC010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 45476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45476
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_514_length_45476_cov_7.095793"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..473
                     /locus_tag="PLM79_03840"
     CDS             66..473
                     /locus_tag="PLM79_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85468.1"
                     /translation="MGAYDYVQKPFKIERITIMTRNAVEKVKILREKNQLLKDLEIVY
                     RKLHLLEEECHSTTAGTPAPQNDHPAANASVLLFPRHTLPLNLFEIHRESPAQVLSKL
                     ERLKELRRDHVISDAEFTLLKRAIINNFGSKET"
     gene            470..1978
                     /locus_tag="PLM79_03845"
     CDS             470..1978
                     /locus_tag="PLM79_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85469.1"
                     /translation="MKILIVEDDVVLSELLREYLQQLDHERVRVCFNGQEAEAAVQEE
                     SFDCAFVDLRLPDMDGLRLLDSIKREDPSLPIVMMSGYPTMGSTIEAMRKGASDFLTK
                     PFTLQDMALALERVTKERRLLLENLSLQLEIQARNQLELVNKELQDKIHEQGQLFGIS
                     RELDEVRSSEDLYPRIVEVASRVTSSKQIGFFIMPPEQNSLMLVSEHGLDRLHDGRRL
                     VQVRGEQLKEALNRESPVRINLSDLFGGTDSETWLRNSSRLSCWPLHIRGELFGFLAA
                     AANGEEKVFSQTEIKLLDFLVKKAALTIENMALYESMIGNFYGILKSLVNALEAKDLY
                     TGKHSERVTRYALRIAQRLGCSPAQIEALQTVGYLHDIGKIGIADSILNKPTVLTSQE
                     YELVKKHPVIGDSIVSELGLSPEERGIIRHHHERWDGTGYPDGLAREEIPHLARIVMV
                     ADAFDAMTSKRAYRDAMSPKDALQELQRNKGKQFDADVVDAFREAGSACDAE"
     gene            2024..2857
                     /locus_tag="PLM79_03850"
     CDS             2024..2857
                     /locus_tag="PLM79_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOV85470.1"
                     /translation="MKSEPTTDGDAPLQKLFEQSLEALFEKDRLSHLGRLVRGLIHNV
                     NGPLQNVSMLAEMLLKGQDQLDRMAPTKPLEALGEWEKALVKQRQRMERLSQQIANLA
                     GILRDFMVLLEIERNESEVDLRLLLCKLVRVLQSDLFLKHEVEVELNLAEELPLVRIL
                     GRDLVPSLMHLFHNCLCALKHGTRKQLTVDCFLEGDLIKILFRDTGCGVDPRKVQGPY
                     FDLFFSDWPPPSAANENDDKHLGFGLFCVRRLLGPYGVSIRMEPQDPGTLVEVLIPVQ
                     A"
     gene            3288..3761
                     /locus_tag="PLM79_03855"
     CDS             3288..3761
                     /locus_tag="PLM79_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85471.1"
                     /translation="MKILETQRNESMEKTREASRPSRPGPSGAFQELLNTEVGDGPRT
                     TDPSPPGVSLTGLDSLNAMSLLTPISGTCPKDPAEIALSVGRNLDRLARELEGPGSSE
                     PKRTEALIESLRSEAERLREETRSLPEDHPLRKISDELSVTAYVESIKWNRGDYL"
     gene            4750..5571
                     /locus_tag="PLM79_03860"
     CDS             4750..5571
                     /locus_tag="PLM79_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase"
                     /protein_id="HOV85472.1"
                     /translation="MNLTDELFQRYSDLVYQECGINLHEGKKALLQARLNKRLRATGF
                     ASYEQYYEHITSPGNSGEFIHFLDSISTNLTYFFRESQHFDFLNKRLPELLETKIKEK
                     NPKLRIWSAGCSTGEEPYSIAMCVIPHLEGLPRIDFRILATDISTHALNTAMRGIYSE
                     EKAGKVPEAFRSQHFRTLKNHNGRREFEVASALRDRITFGRLNLKDPFPFKGLFDFIF
                     CRNVMIYFDKKTQEMIVHKLARYLSPGGYLLVGHSESLMGLSHSLRYVCPAVYRK"
     gene            5606..6085
                     /locus_tag="PLM79_03865"
     CDS             5606..6085
                     /locus_tag="PLM79_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOV85473.1"
                     /translation="MRRVVGVADMAVSNNPAENLVTYSLGSCIAVVLYDATVTVGAML
                     HYMLPDSNLDTEKAGRVPAMFADTGIPLLFRQSYQYGATKKNLVVKVVGGAQILDENG
                     LFNIGKRNYLALRKIFWKNNVLIKAEHVGGNVNRTVRLDMKTGQVFLKVSGIGEFEL"
     gene            6117..6539
                     /locus_tag="PLM79_03870"
     CDS             6117..6539
                     /locus_tag="PLM79_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85474.1"
                     /translation="MPYNVLIVDDSRSMREVIKKVLRLSGFQIGDCLEAGNGEEALSV
                     LANHWVDVILSDIHMPVMDGFGLLQALTAQEDLRDVPVVLITTESSHSRLKEALALGA
                     RGYLRKPFRPEEVRALLAQIMGETDGSQAGNVDQGCDF"
     gene            6652..6957
                     /locus_tag="PLM79_03875"
     CDS             6652..6957
                     /locus_tag="PLM79_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85475.1"
                     /translation="MEIGIAVTESFGRMIAANFLGIREEGVKSEELEDVLKELVNMAG
                     GNYLDRADSENWQLEIPRFRRFAQSSSATLWGMPMDVLGEPAGLVFIESGSFCSQSS"
     gene            6977..7492
                     /locus_tag="PLM79_03880"
     CDS             6977..7492
                     /locus_tag="PLM79_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOV85476.1"
                     /translation="MENTGPLSHQEYFLRPGYIFVTQRPTLISTVLGTSVAVALWDRE
                     KKWGGMNHFLYPLVPDPHKATAQFGNVAVRALYNLLVREGSHRSDLDAQIFGGAHLAE
                     ASPEEIEVSRRNITVGREVLRKIGIPIISEDTGGTRGRKLVFNTATNEVAVFRAERLR
                     DSDWYPYRDKR"
     gene            7502..8575
                     /locus_tag="PLM79_03885"
     CDS             7502..8575
                     /locus_tag="PLM79_03885"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOV85477.1"
                     /translation="MQKASNPIKVLIVDDSAIVRKIFTQELSKHEDIEVVGTAPDPYV
                     ARDKIVKLRPDVVTLDIEMPRMDGISFLRKLMKYFPIPTIIVSSLTPKNSEMALEAME
                     SGAVEVLAKPGGSYSVGDMSIQLAEKIRAAAQVRVRSDLGTGPGARSPEPVEENASLS
                     RALKRTTHKVIAIGASTGGTEALKVVLTQLPSNTPGIAVVQHMPPRFTTAFANRLNDL
                     CQIEVREAKDGDSVLPGLALIAPGNYHMVLRRSGARYYVEVKTGPLVCHQRPSVDVLF
                     HSVAQYAGANAVGVILTGMGRDGAGGMLEMKKAGAPTIAQDEASCVVFGMPKEAIRMG
                     GVQQVVSLQQIPHTVLKAVASDG"
     gene            8758..9141
                     /locus_tag="PLM79_03890"
     CDS             8758..9141
                     /locus_tag="PLM79_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85478.1"
                     /translation="MAYKDMTALVVDDFATMRRIVRNILRDLEFKNILEAEDGTAALE
                     VLKTNKVDLIVSDWNMPKMNGLDFLKQVRAAEQTKDIPFLMVTAEAQKENIIEAVKAK
                     VSNYIVKPFTAATLGEKLAKILPND"
     gene            9185..11401
                     /locus_tag="PLM79_03895"
     CDS             9185..11401
                     /locus_tag="PLM79_03895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOV85479.1"
                     /translation="MQDVVEKLTNGVIDGPEDLMGMGECLNLLEQLEAASYFSGEAAD
                     LLPRIRQLLNRMILEESPDLQKDWQTVQGLIRELCPTGNGGPESSEDSVSHSDLEAIK
                     DLIREPPAGRTDPVGPLPATSETAGEWEGGSEGGSRAPEGSTAEMELQDPELVRDFIE
                     EAKEHLSSIELNILALETDPKDQDAINAIFRPFHSIKGVAGFLNLKDIHHLSHEVENL
                     LDCARAGKILITDDAIDLILKSVDLLKTLIGVLEDSVAQGEPLPDNAEVVEDFLQRLS
                     AFQDTPVAEEAPVRKLGEILVDRGVVEDETIHRALEDSRASGKKLGELLIGKGMVSSR
                     EVSSALREQRRSKESAASVRIDTHKLDNLVDMVGELVIAQSMVLQNSEVEAIKEQKFQ
                     KDTVQLRRITNELQRISMAMRMVPIKSTFQKMIRLVRDLSRKSGKEVTLEMKGEETEI
                     DRNMVEEIYEPLVHMIRNSIDHGIESPEERKAAGKVLPGVIVISAEQKGGNIVIEIED
                     DGRGLNAEKIRSTAVQRGVISPDEPLDERGIYDLIFHPGFSTKDKITEVSGRGVGMDV
                     VKKSVERLRGKIEISSHRGRGTRFQLKLPLTMAIIDGMIIQVGGQRYVVPTISMKESL
                     RPGREAYQTVHRKGEAIKVRDTLMPLIRLHQLFGVEPKYRDPWEGLLLVVNEDNRSYC
                     LQADEILGRQEVVIKSLGSSLRHVTGISGGAILGDGRVALILDVKGIVSHYEGNSR"
     gene            11398..12789
                     /locus_tag="PLM79_03900"
     CDS             11398..12789
                     /locus_tag="PLM79_03900"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOV85480.1"
                     /translation="MTMELHEEDWAELVQILETVEEHLGFLDGRPDDQERLDTCRRAL
                     KSFHTTAAMLGLGTLEKAGMGLEKALADQRTPLRQRPDDLYTVGSALNGIIEELKKAA
                     DGGEISAVRVEEILTALDGAQEPGETPPGQVREEEDRHPEGGSSARKPVGTIPGKPAD
                     APAVDFSGLEQVVAHLGGHILYKSNGSNSPAFQLHFEAYPAVVEQLTALLSPIHPEGS
                     LAPELSREDRRMEKILLIIKDFMKAFSEGNLKRAQEALLSLTDQDKHVALYNEIGTMA
                     RQLHSSLTGFTSTLDPALKEMVEDKLPDSGNRLEHILEITEKAANTTLDHVEAIQKRN
                     ERDLELVAQMQDMVRGLQAIGDRAERRLEEGSAMTEKLSASILQTRDDLTTILTAQDY
                     HDLTGQVVMKILHLLKDLEMKLVNVIQAFGVPMEGRRKGPSDELYGPAHKKRTEALHS
                     QDDVDALLADFGF"
     gene            complement(12913..13425)
                     /locus_tag="PLM79_03905"
     CDS             complement(12913..13425)
                     /locus_tag="PLM79_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOV85481.1"
                     /translation="MEQLARTLDKAKRTSAEKGGKYLTFTLAEEEYGIGILKVKEIIG
                     MMPITLIPQTPHYVKGVINLRGKVIPVVDLRSKFSMDATDYTERTCIIVVEIASDSGS
                     IMMGIVVDSVSEVMNIKNEDIEETPTFGTKLNTDYILGMAKMNGGVKILLDIDRVLTA
                     GELDLLDKAV"
     gene            13668..13895
                     /locus_tag="PLM79_03910"
     CDS             13668..13895
                     /locus_tag="PLM79_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85482.1"
                     /translation="MVITDYRIQSVLRTYSKQLQRSKITKRLESEDPKRPQEKVTISE
                     EARRRAIVERVTSQAMEQIYQQSEDFDLLKG"
     gene            13973..14266
                     /gene="flgM"
                     /locus_tag="PLM79_03915"
     CDS             13973..14266
                     /gene="flgM"
                     /locus_tag="PLM79_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOV85483.1"
                     /translation="MDVKKVQAYITQAPLKTAEPGSRTPGEEKAQASSVEADSVKLSK
                     GYLEVAQAKKLVMDRAEEIRIDKVEAIRSSVDNKTYAIDAEKIAQKMTEELWW"
     gene            complement(14453..15364)
                     /locus_tag="PLM79_03920"
     CDS             complement(14453..15364)
                     /locus_tag="PLM79_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOV85484.1"
                     /translation="MRISFATAWDRMLLNINRDSVDLDRLTTEIGSGKRLLEPSDDPA
                     AWSQKVEMKQGLRELQTFKDNVDFGIGWNQAVEDALNRASDLLMRAKEVGMGAVKLVD
                     AEERSAKVDELQQIFEEALGVMNEQYGELYLFSGKQTSTAPFQLGDPTDPGYPDFTYH
                     GDTQDYQVRVGKDFRQTINLNGADVFFADASDVDTNLLKRIMDLRDAVREGDTDKITS
                     LLGSLDEDYERITMKQSTVGVRLEGLDRQKSALESIAVNRTDRLSEIEDADVAEVITR
                     LQQKQVAYEAALRATTLIKDLNLAKFL"
     gene            complement(15378..16799)
                     /gene="flgK"
                     /locus_tag="PLM79_03925"
     CDS             complement(15378..16799)
                     /gene="flgK"
                     /locus_tag="PLM79_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOV85485.1"
                     /translation="MSGLMVTLETAKRTLLTCQVCVQTASHNIANAESKDYARQRVAQ
                     ETSTPYQVRDTWLGAGARIRSVVQVRNEFIERRLTAAVSKEADYRTRSTQLSLIEATW
                     ADDGEQGLSQALGAFWDAWEALNRNPDGISQKALVEEAVQSLVDRVRDAYRGMTEIAV
                     GIESEAKSGVTKVNGLLEAVAAYNEQIMKAESTGQPANDLRDLRYEKVQDLAELIPVR
                     WDEEDNGTLTLRVTDYGSDITLVSAGDFGSLVYDDANHRVTYRDYQGTAVPDDPTLQN
                     ELSGGKLAGLLSVYNLAGNQHDLSFVQANPDAAELTYLDRLNAFAAALITEVNAIHRQ
                     GGGSVVFDDSVLGEDFKASDLAVDANFVVNSAQALEIADLQSHRFSGLPGESNLGNAT
                     FLEYLGRIQEQVGLDRQDSLSWADFQATLLNRLQEEQQSVSGVSIDEEMVDLIKFQQI
                     YQAAAKVISHAAEMLDTAIEMVG"
     gene            complement(16801..17265)
                     /locus_tag="PLM79_03930"
     CDS             complement(16801..17265)
                     /locus_tag="PLM79_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85486.1"
                     /translation="MRPREADKRVPAEGECSPLQGELLGVCSLKAEQLLQVLEEESSV
                     LRAFQTEQLLPLLSRKEALTLELGESVGELARLKALNGAAADSPAYKRLAERLKEIKQ
                     KNRSNQVFVEGSLLYLQDLLKCLRPSTYLPGRGGGPQAAAIAVKGLALRKEA"
     gene            complement(17364..17678)
                     /locus_tag="PLM79_03935"
     CDS             complement(17364..17678)
                     /locus_tag="PLM79_03935"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HOV85487.1"
                     /translation="MLPGVSPELGVAPMDPELKKKRLKEACREFEAIFANYVLKSMRE
                     GIQRAEEPDQARQVYESLRDESLARELSRGPGCGLAELMYRQLLPLVEAGEKPQNPVR
                     NP"
     gene            complement(17693..18802)
                     /locus_tag="PLM79_03940"
     CDS             complement(17693..18802)
                     /locus_tag="PLM79_03940"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOV85488.1"
                     /translation="MGRTGTRTLPAALLLTVLFLASVGHSARIKDLAYFRGTTSNPLI
                     GYGLVVGLNGTGDKDKTMFTVNTLANLLDNVGIRVDPTQIKVKNVAAVMVTAKLPPFA
                     RVGTRIDIQVSSIGDAKSLEGGTLLLTPLQGPDAKTYALAQGPISVGGFSVSGQSGST
                     VQKNHPTVGFVSGGATVEQEVQVHFTNVQQLDLILKKPDFTTATRAMSLVNSLVPGAN
                     AQAVDAATIKLTVPASFQGRVPEMITQVENLEIQQEITAKIVVNERTGTIVMGESVKI
                     HPVAVAHGSLTVQITEQQAVSQPLPFSRGETTVTPQTRIEVQEGKGALSMVGGVTIGQ
                     VVKGLNAIGASPRDLINILQTIKAAGALQAELEII"
     gene            complement(18813..19610)
                     /locus_tag="PLM79_03945"
     CDS             complement(18813..19610)
                     /locus_tag="PLM79_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOV85489.1"
                     /translation="MHPQANDSSHPEERKRGRFSPGRLAVFPLVISGLFLVSCATTQP
                     SPPQSGPVVRSPVPMTPIPPPTPGDAPEAPTGSLWAAGGSGSLFQDLKARKVGDIVTI
                     TVSEEAKGSKEATTKALRSKDLSGTFTFGGAGLTSGGGTGAAGGGGVPGATFGPYSGQ
                     FGTGFTAAGATTRSDSMTAYMTATVVDVLPGGNLLIRGSRWTKVNDEMQQIVLEGIIR
                     PNDITRHNTVLSQNIAEAKIFFVGKGPVTQHQKPGWAFQLLDLISPF"
     gene            complement(19733..20761)
                     /gene="flgA"
                     /locus_tag="PLM79_03950"
     CDS             complement(19733..20761)
                     /gene="flgA"
                     /locus_tag="PLM79_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOV85490.1"
                     /translation="MRAKTFFWAVLLLGAGFSGFFPFPPGDCAGPESVCWAGASAVKI
                     EFSSSVLVHRKRVTLWDLCNPEGIPGDWMALLSSIDLGEAPSPGTEKYIEAPQLKSFV
                     HRVLSSRGKDPAQWELSIPDRILIRRESMQVSREQIEGIYRDYVLSRGPWSPQDVVIR
                     GIQYSGPVDLPAGELSYEVTSHPQERFMGNVAVTIQFSVNHEKDRSLRVSGKVDVFQD
                     VVVTSQPLKRNDVVSAKDVELQKMNISESPDRFATKIDQVVGKRVLRDVGLRQAVPLA
                     DLDNPPALKRGAAVTMLYDLPGLRLTAKGQVREDAGVGDTVRVINVMTNRTVHCRVLD
                     PTTVRVIP"
     gene            complement(20778..21560)
                     /gene="flgG"
                     /locus_tag="PLM79_03955"
     CDS             complement(20778..21560)
                     /gene="flgG"
                     /locus_tag="PLM79_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOV85491.1"
                     /translation="MIRSLWTAATGMAAQQMNMDVIAHNLANANTTGYKKSRANFEDL
                     MYQNLTPPGAETSNNTRLLNGVQMGMGAKTVSVQKIHTQGSFTQTGNQLDLAVEGRGF
                     FKVLRGTDEVYTRAGAFKMDQDGYICDAEGNRLQPEISIPKGTVAINIDAGGNLTAVD
                     QEGKALVTQRLNLFDFPNPAGMISLGRNYCMPTEASGDSETGQPGDDGFGTLLQGFLE
                     NSNINVVEEMVNMIVGQRAYEANSKVIKASDDMLQMANNVKS"
     gene            complement(21573..22271)
                     /locus_tag="PLM79_03960"
     CDS             complement(21573..22271)
                     /locus_tag="PLM79_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HOV85492.1"
                     /translation="MRIGIYGASIAAMHQEKTMAVVANNIANAATPGFKRESVHFKDA
                     LHQNTHTQMAQGRIQPTGNPLDIALVGEGFLRVQAPEGILYTRAGNLTLNSEKMIVTQ
                     QGWPVLGQNGPIRVEHSNVRIEKNGQIFDDRGKTEEQELVGTLDVVKFSPQARLEKVR
                     GGYVKPVEDEAPLPAEQFTVEQGALEESNFNLVEEMARMIESSRVFEAYQKTIQVFEQ
                     QEAQLINKLGNPNG"
     gene            complement(22513..22986)
                     /locus_tag="PLM79_03965"
     CDS             complement(22513..22986)
                     /locus_tag="PLM79_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85493.1"
                     /translation="MDWFFHQPHALTLLQILLDVLLILFLACLFFRRFRRDRNVEELT
                     RSFGKIVEETQRIAAEFETNLQERRQLIQQFLAKLDQQLERAQLMSLKLEALQNQGPL
                     PPPHGSPPQPRASQNDQILQLARKGMDATSIARHLQKPVGEVELVLKLQTITGKR"
     gene            complement(23015..23779)
                     /locus_tag="PLM79_03970"
     CDS             complement(23015..23779)
                     /locus_tag="PLM79_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85494.1"
                     /translation="MGREIARKNIDEELVPSGAQFDEKVLEKIEEEFDQISPMGGQSL
                     FEGPSGGNGNSKAASDKDFSLDDLEGFSHESLSRSFKEETPAPAALETEGAAVDQEED
                     PGEQRRRVPKRKILWLGAAAFLLLGMVGFACALWWPTSNGSAPVRLVRKPVPIPRFPG
                     KVQLLLPLKVGKKADILSLELELEFLGERSFEQFEQSPTMLRDRIYRYLLHEHPELLS
                     YTHWQETVEKKLFSHLKESLPATGIGAVRLLQMDVL"
     gene            complement(23797..24603)
                     /locus_tag="PLM79_03975"
     CDS             complement(23797..24603)
                     /locus_tag="PLM79_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HOV85495.1"
                     /translation="MTQRSYPARNAYGTARFDSSPAVPDKERMILQHSDLVKYMALRL
                     VSRLPNNIQVDDLYNAGIIGLIDAIDKFDPGQGIQFETYAKIRIRGAMLDEIRSMDWV
                     PRSLRQKSSELERTCLALEQRLGRDASDEEIADQLGISMESYFKLLDEIKGISILPED
                     IHDAFQENRGSAFLATESDELFRLIHQKELRAHLAEAIRSLSEKEQQVLALYYYEELT
                     MKEVGAVLGYTESRISQIHTKAVLRLRTRLARKLRREDLPEYLEPREAVV"
     gene            complement(24629..25516)
                     /locus_tag="PLM79_03980"
     CDS             complement(24629..25516)
                     /locus_tag="PLM79_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOV85496.1"
                     /translation="MDQAVSLRERKAGPIAWSKKSLRIADPQGPLVRVLSVSSGKGGV
                     GKSSIVVNLAVAFAQLGKKVLIMDADLGLANIDVLLGLTPKENIAHVLEGKRQLREVI
                     INGPGGVRIMPASSGVQQLTRLTDEQKLLMLEMLDELESEIDIFLVDTGAGISDTVLY
                     FNLAAQEKIVVITPEPTSLTDAYALIKVLYTRYGERHFRVLANFVEDEKAGKGIFAKI
                     STVADHFLDGISLDYIGSIPRDPNVPRAILQQRPLLHAFPRSAAARAFLSLAERIRKS
                     PPHQNNGNVQFFWKRLLQV"
     gene            complement(25518..26597)
                     /locus_tag="PLM79_03985"
     CDS             complement(25518..26597)
                     /locus_tag="PLM79_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV85497.1"
                     /translation="MQVKTFRAPTIGQALEQVKRELGQDAIILGNRKVALSAEESVVE
                     VMVALEREAEVSQASNESMRNGIEEIKGLLSLLISSKDTFARLQLEQPVTELYHSLLT
                     RGLDEKQTFLLLKKALRQVQGNPHEKERIADAFRAQLAEKIRTCRPFAGSFLHADEPQ
                     LYAFVGPTGVGKTTTLAKLAAHLKIKRRLDPAVISLDTYRIGAVDQLRTYADILAIPL
                     VVAQSREEFHQAVERLRGHPILLVDTIGRNFLRKQHVADLAGTLGGSRQVHSFLVLNA
                     TAKDEDLKQTIQHFRPLDPHSLIFTKLDETMSHGCIVNQLLRFPYGVSYLGIGQRVPE
                     DIEAATHKRLLSLLIPSGNGSSRKE"
     gene            complement(26657..28756)
                     /gene="flhA"
                     /locus_tag="PLM79_03990"
     CDS             complement(26657..28756)
                     /gene="flhA"
                     /locus_tag="PLM79_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOV85498.1"
                     /translation="MAVATEASKPILQRLGSISDSDAVMGIGIVIILSVMLLPMPAPM
                     LDVFLAANLGVALTIILTTVYVVRAVEFTIFPSLLLVTTLFRLSLAVASTRLVLLHGH
                     EGTTAAGRIIHAFGNFVVGGNYVVGLVMFVLLVIVNFMVITKGTERISEVAARFTLDA
                     MPGKQMSIDADLNTGIITETEARSRREMIAREAEFYGAMDGASKFVRGDAIAGILITV
                     INILGGLAIGVLQNGMSLVGAMMNYTLLTVGDGLVTQIPALVISTSAGILVSKAASDS
                     GMGTTFSKVFRMQPRPLGITGGMLIFLGILPGFPALPLFGVGGVLVAASLLLQKGSQP
                     LDGGGDEEGAERDRPEEVAKLPPLPDVLELEVGYGLIPLVDESQAGDLLARIKAIRQQ
                     LALEYGIIVPALHIRDNLQLRPAEYRLLLRGNVVARGELVLGHSLALSSGEVSETVDG
                     IPTKDPAFGLPALWVPENKNEKAIRAGYTVIDISTVLATHLTELFKKHADELLDRQTV
                     QQLLDHLAESQPKLVEELIPNPLTVGTIQKVLQNLIHEQVPIRDLQTICEALADHAGI
                     SRDPDILTEYVRQALARTITRPFMDDNGRIHILTLHQPLEDQLVRSVQKSDQGSFLAL
                     DPGFLHQLIQGVGAETRRLAGKGHHPVILCSPMVRRHLKKLLDRFMPEVAVISHSELG
                     HTLEIQSAGMIRMDQAA"
     gene            complement(28765..29817)
                     /gene="flhB"
                     /locus_tag="PLM79_03995"
     CDS             complement(28765..29817)
                     /gene="flhB"
                     /locus_tag="PLM79_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOV85499.1"
                     /translation="MASSQEKTEKPSSKKLGQARSKGRVAKSTELNHLVVMGLGGFVL
                     LLSSPAILDHYRQMLHGLWASGFRTPWSQALFSQMVSTLFLMIAPVVVTCLFAGVVIN
                     LFQLKGVILAFSILKPDLSKINPLGGFGRFFSLRSLLELAKSCIKVFIIGYVIYGALR
                     SETEQIAALTHLDVKDILAVFGSLAWKVFVRVCGVMLILSVLDFGYQKWQYLRDLRMT
                     KQETKEENKQSEGNPQIKGRIRSIQRNLARRRMMSAIPQASVVVTNPTHFAVALLYNS
                     KMEAPRVVAKGLDHLALQIIKIARYHGVPVVQNPPLARALYQQVKLEETIPVNLYKAV
                     AKVLAYLYQRRKQKAG"
     gene            complement(29886..30656)
                     /gene="fliR"
                     /locus_tag="PLM79_04000"
     CDS             complement(29886..30656)
                     /gene="fliR"
                     /locus_tag="PLM79_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOV85500.1"
                     /translation="MLNLSIPSVEIRVLLAIVLRLSIVFVMLPFFGGVQVPRTIKIFA
                     ILALSAMIYFLPGTALKPLSLDPIELVRVVMGEFVFGVVLGLSLLIVFGAFQLAAEMI
                     SFQMGFGFAQAVDPQSGTQVPLVSGWFQVVAMLILLSSNGHHVILKALVESFTRVPVG
                     GFVLGADTVQQVLGFSVQLFVLGMKIAAPVTISILLAHMGLGLINKFAPQINILVASF
                     PVTILVGFLLLGASVSVWGIAMRDSLLRFSQVLRVFFQ"
     gene            complement(30700..30969)
                     /gene="fliQ"
                     /locus_tag="PLM79_04005"
     CDS             complement(30700..30969)
                     /gene="fliQ"
                     /locus_tag="PLM79_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOV85501.1"
                     /translation="MSTEAVLGIARQALETMLTVSLPVLALSLVVGLMISIFQAVTQI
                     QEMTITFVPKIVVTFLSLLLFGAWMIGRITDFTREVFQLIPQWIH"
     gene            complement(30982..31803)
                     /gene="fliP"
                     /locus_tag="PLM79_04010"
     CDS             complement(30982..31803)
                     /gene="fliP"
                     /locus_tag="PLM79_04010"
                     /inference="COORDINATES: protein motif:HMM:NF009438.0"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOV85502.1"
                     /translation="MSPPESGPGARRAGRNRVGNRRSPWGKAVLIVLAVLTVQSMAWC
                     WAADLRLPGLRVDLDNAGGPRELSGILTVVLLLTVVSLAPAILIMTTSFTRLAVVFSF
                     LRHALGTQGSPPNQVIIGLSLFLTFFIMQPVWQKIHQEAVVPYQRQEISGDEFLKRGL
                     KPLQDFMLKQTRKQDLGLFVSLSQQEKPANAEALPITTVIPAFVISELKTAFQIGFLL
                     YLPFLILDMVIASILLSMGMMMLPPIMISLPFKLMLFVLVDGWNLMIGSLVKSFH"
     gene            complement(31800..32117)
                     /locus_tag="PLM79_04015"
     CDS             complement(31800..32117)
                     /locus_tag="PLM79_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOV85503.1"
                     /translation="METMWDLGAQAVRVAVSLGIVLSVLGAVFYGIRAFGKKVRGTGA
                     DPWIKIQAQHPLGLKHNLLLVRVQEQLFLLGLSPQGIHFLTSVRETAGNPGGSAADGA
                     ENP"
     gene            complement(32209..32583)
                     /gene="fliN"
                     /locus_tag="PLM79_04020"
     CDS             complement(32209..32583)
                     /gene="fliN"
                     /locus_tag="PLM79_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOV85504.1"
                     /translation="MAAKAHDEMNPLNPPPVSPEKGAAQFPGLDQDATRRAAVRDMEF
                     LLDIPLEISVELGRTTMIINELLQLGQGSVVELNKLAGEPLEILVNNRLIARGEAVVI
                     NEKFGVRITDIVSPIERVQQLA"
     gene            complement(32774..33757)
                     /gene="fliM"
                     /locus_tag="PLM79_04025"
     CDS             complement(32774..33757)
                     /gene="fliM"
                     /locus_tag="PLM79_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOV85505.1"
                     /translation="MEKILTQEEVDSLLRGLSNGEVETKQVQEEESGGIRRYDLTSQD
                     RIIRGRMPTLEIINDRFARVHRVALSSALRRLVDITVTQTEMIKFGEFVRTLPVPTSL
                     HVLKMEPMRGHILLVVESRLIFNLVDCFFGGTGRSNFKIEGRDYTSIEHRVIHKVVQG
                     VLKDLDHAWKPVINISFQFVRSEVNPQFATIVPPSDVVIVVHFELELDRMIGKMILCL
                     PYSTIEPIRAKLCASYQSDQLEVDVGWIERFKKRLCEVGVGVNAELGKTWIKGRDLLH
                     LEVGDVLMLDQDVDEPLVVRVGGIPKFKAFPGTSKGNMAFRVTETINPPGF"
     gene            complement(33776..34195)
                     /locus_tag="PLM79_04030"
     CDS             complement(33776..34195)
                     /locus_tag="PLM79_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOV85506.1"
                     /translation="MKIILLAVILGLLGGGGYFGYVKFVKGPSDAEAAKPQKQEKIIC
                     ELEPFLVNLADVGGKRYLKVTLKLEVNNPLVPAEVTTRGFEVRDSILMLLSGKEYNDI
                     ASSGGKITLKREIISQLNRILKQGQIREVYFTDFIVQ"
     gene            complement(34656..35453)
                     /locus_tag="PLM79_04035"
     CDS             complement(34656..35453)
                     /locus_tag="PLM79_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HOV85507.1"
                     /translation="MGINQAMFNGLSGLNGYSSAISVVSDNIANANTTAFKSNSIQFG
                     DMVNSYYSIFASGTQGAGVGVQVMGFATDYAQGTLLDTSKWSDLAMSGEGFFTVVDPV
                     SSKTFYTRDGSFHLDRDGYLINNHGYRVQGYDPAGTLGDIQIANPTDYISIRVEGNGD
                     IVAVDDTGTDNTLYTLGLKSFSNKDGLVRNGGNLYITGPEIGQTFDNATNPEIFGLVN
                     DFSLEGSNVDLARQMVDMIIYQASYNANSKTITTSSQMIDTSINMVR"
     gene            complement(35485..36210)
                     /locus_tag="PLM79_04040"
     CDS             complement(35485..36210)
                     /locus_tag="PLM79_04040"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOV85508.1"
                     /translation="MSIVTNQVTGGFAGGNVTQSAKSTLSDMNSFLLMFTTQLKYQDP
                     SNPLESYELASQLAQFSTVEKLTQLNENVKLQQTQLASINSAQMVDMIGKEVVGSDNK
                     IHLADGQCSKASYQLGSAAQQVKVVVRDAAGQAVRTMDLGSLQAGRYDLQWDGRNDAG
                     EMMRNGSYQFSVEAVDANGNVVDASTSVSGKVYAFRMVEGVPYLVLDSQDGVTLAIGS
                     VTEVHQEAKESILSLENPIISLS"
     gene            complement(36255..39047)
                     /locus_tag="PLM79_04045"
     CDS             complement(36255..39047)
                     /locus_tag="PLM79_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOV85509.1"
                     /translation="MQTNHLNAGRLLMGVLSNEHGKLGSKILDAQFSFSRELDAQNQE
                     KMFVLGSNPSSMRGNDAALEMFAAGTGDSQREPVQPAGTAKPVAGGSCNGGVVVLSHA
                     GPETPVEPPEKAAPETEALERALPRKPTRISRERVKEALYFTNPTASEKIMAHLQLPA
                     EARKACKDAADSQGMLSYKDLLSILQAQKTPRERLDGKEPEVSARNVRLLLDSLQYKS
                     PTGTPVSCKIQPLMKESYNLQEFVELLDRVVRQVHRERSKTEALSLQSVPPATGLTEG
                     SSAIGSVSDLVRADHVERMSDTRLPSFCLNQEPLRGLRAKTVDGPAVPASDLKDGDRG
                     SESAVSVETPERKEADQVSRVPKDSDQFPGLPISGEEESFRGWLGDRRGSEDRGSHPQ
                     GSLRGLSGQEAVSRRSVLPGVPETFASLAAMDLSGKDAESTPGHQGPPPSSPSVQTVE
                     TAGDRPFSSHKSPSTTEGSDSRVQPAPEPGQGFSHQFDGAGGDAAGKFGRSIKDSPSP
                     GITDKRTRDIDLFEVERTVPESQRPREGTGAQSGGSRLSDLLVGSEREGEELRVTERM
                     VSMAEAIGESSRRVQGRPGPIGPLSEAVPAGSERSRSEMRGTRMPLSEAREIRSESER
                     PRYKLTMEKVPVPEATDAGANEQVLEGEPNRLPVLEKAVPGEEPSNGDQSEGFQWKSI
                     WRRFTARSPRPLEPAASAFNSDSADTQETTGREGSSPETNGRFPSEDTSARFSAHETK
                     PSPSVSPTQSSEPWEGPGISRDGFVQQLRGGTPEAAAPASSDETGHLSLRDASWPRGL
                     AHEFKELTTRKGNHLTLELEPKDLGRLTLTVETDRNHVTAWVSTETEQARLLLSQHTS
                     ELRQCMQEQGLLLGEFFVGVKDQKGHGTLYRQGRSAKKVAELGGKTAKGYGKEIASKT
                     HVYGVRSGDQLLSVVT"
     gene            complement(39468..41807)
                     /locus_tag="PLM79_04050"
     CDS             complement(39468..41807)
                     /locus_tag="PLM79_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOV85510.1"
                     /translation="MPELPDPLSPDRRSFLMGAAWAVGAAFAPRLALEAMGQTTKPPA
                     PCSEPSAEGACCNDSKCFPTRDKVPDPENLSFWVRYPGKDPVRLEGHYWYNADAAKEG
                     RKFPALVELNPYRRRDGMMLGDSAFYPYFAYHEYLCFRVDLQGSGDSGGILTDEYTEE
                     EVLYCIQVIEQVARHPLCNGKVGMMGKSWSAINSLMVAAHDRCPSELKAVLVICGSDD
                     RYNDDVHYMGGAMMQDNVGWPSSMWGWIAQPPDPEIVGNRWKQMWRERIRNADFWFKH
                     WGSHQTRDSYWSSSSVRDRYDRVKVPVYIVSGYLDGYKNPVVRAVEGLHSAGMAVQGL
                     LGPWGHAKPHAGDPGPRINWLPYLLHHWWDRWLKDADPNPREELPELTVWLGESREPS
                     RSCCTPDRGKWASEDAQWPTRVREKVFYLWPNQSLSPKEPGHPGYLICPGPLVLGTKM
                     LETSSWGQCGNDDLPGDQTEADNQSLHFDSGPLAEDMDCFGYPTVSLNLVCNKPLASV
                     AVRLCEISPYTGASHLVTYSFFNLCYRDGDMAAPRPVSPGVPFHVSFPLNVFGHTFKR
                     GWRVRLSLSPSLFPTLWQSTENPILMVVTGRSPDSPASALSLPGRRPRAQDRRLKKLL
                     PSDPQILCVDSSEYVPTVESRKSRYLCKVKPITRGKKKGVLVRKVTDSGRYCYGGPLR
                     DLWVDQVSQENFRIFHDDPLSMTGLTASRTSMKRAGEGGEWKIKLKTATRVWTEKDSS
                     GKYFFRYTATIKTFIANARGEFEPFEHKTVKGSIPRLWV"
     gene            complement(42181..42822)
                     /locus_tag="PLM79_04055"
     CDS             complement(42181..42822)
                     /locus_tag="PLM79_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85511.1"
                     /translation="MSYKKSKYPKSLIGAVFLGFFLLSLKLAVTVGSLLLQKDGAEAP
                     PFPAEVLAEDSKAKPVPSTPPVKADPAKPAPTKVASSGPGAQPVTDLASYLERKEAEL
                     KQREEKLRQKEEYLSQMEQEVERKFKELIPIQKEIQAYRTEKEETQNNKIRSLAKIYG
                     SMKAKEAAKLLENLEENLVVHIISTMNSDEAANILSHMDVKKAAKISESLSHR"
     gene            complement(42803..43282)
                     /gene="fliJ"
                     /locus_tag="PLM79_04060"
     CDS             complement(42803..43282)
                     /gene="fliJ"
                     /locus_tag="PLM79_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOV85512.1"
                     /translation="MPFQFRFAIVLRHRQHLLKQAQVAMAAAQARYDALESRIRAVSA
                     EIAGQVQALEARQSEGMTVSDYLSHKEFLQSLERHLLLLRHDRQNALRAVENRKKELL
                     EKRKDVRMLELLEEKDREEYRYLLGKEDRKRMDEIASYQESPDKITTRSEEDELQEE"
     gene            complement(43419..44783)
                     /locus_tag="PLM79_04065"
     CDS             complement(43419..44783)
                     /locus_tag="PLM79_04065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HOV85513.1"
                     /translation="MKLRPNGQIKMILPNPNYLGFKDPTSLKDCPRYEKYGKVVHMVG
                     NMIEADGLDCSLGDICRITPSRGANPIFAEVVGFHQKRLKLSPLTRLRGIYPGCLVQP
                     YQLAGSVNVSPSLLGRVLDGLGQPMDGLGPLPPGIPYPLSGNVANPLDRPIITEQLDL
                     GVRCINGLLSVGKGQRIGIFAGSGVGKSTLLGMMARYTSASVNVIALIGERGREVNEF
                     LQHELGPEGVKRSVVIVATSDQPATLRLRGAFMACAVAEYFRDQGMDVLLMMDSATRF
                     AMAAREIGLAAGEPPTTKGYTPSVFSRLPRLLERAGNFKEASITGIYTVLVEGDDMED
                     PIADTLRSILDGHIVLDRRLAQTRHYPAIDVLKSVSRLTNRLQDEEHKVLSSRFIQTL
                     SHFKQSEDMIRIGAYVKGSHPDTDYAIEMIDRLNGYLTQPVEEYCSIEQAREQLRTLG
                     LGRE"
     gene            complement(44734..>45476)
                     /locus_tag="PLM79_04070"
     CDS             complement(44734..>45476)
                     /locus_tag="PLM79_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOV85514.1"
                     /translation="CGRNGGDPRDGRHIPFDFRSIEGDVAAVSGVSSVPAASPSSKAP
                     APPEDVESLIQKRLMDAERRALEIEKEGYDKGYEQGVKDGMEFGRKSMLIAQEHLQRV
                     VSRLEALPSRIIEDYREWFVQTVLAAVRHMVRKELDAHPEILLQTLESLLHELGEDSP
                     VTIFLHPKDVGLVEKALEDGEAGFSPARSRICKPDPGLERGDCRIETPLQLLDSTLDT
                     RLALLEKALTGADEAQTQRADQDDPSES"
BASE COUNT         9206 a        13696 c        13350 g         9224 t
ORIGIN      
        1 cacatatttg acacaaatac catgaaaata ttgacagttt tgcaacaggc tccgaggcca
       61 ttcgcttggg ggcgtacgac tatgtccaaa agccgttcaa gatcgagcgg atcacgatca
      121 tgacgcgcaa cgccgtagag aaggtcaaga tcctcaggga gaagaaccag ctcttgaagg
      181 acctcgaaat tgtgtaccgg aagctccacc ttctcgagga ggagtgccac tccaccacgg
      241 ctggaacccc ggcgccgcaa aacgaccatc cggcggcgaa tgcctccgtc ttgctgtttc
      301 ctcgccacac cctccccctg aatctcttcg agatccatcg agagagcccc gcacaggtgc
      361 tctccaagct cgaacggctc aaagagctgc gaagggatca cgtcatcagc gacgccgaat
      421 tcactctgtt gaagcgagcc atcatcaaca acttcggatc caaagaaaca tgaaaatcct
      481 catcgtggaa gacgacgtag tacttagcga gctcttgcgt gagtatctcc agcagctcga
      541 ccacgagcgg gtcagggtct gcttcaacgg gcaagaggcc gaagccgcgg tgcaggaaga
      601 atccttcgac tgcgcctttg tggaccttcg cctgcccgac atggacggac tgcggctcct
      661 ggattccatc aagcgggaag accccagcct tcccattgtg atgatgagcg gctacccgac
      721 catgggttcc accatagaag ccatgagaaa aggggcgtcg gactttctca ccaaaccttt
      781 caccctccag gacatggccc tggcactgga gagggtcacc aaggaacgca ggctcctgtt
      841 ggaaaacctc agccttcagc tcgagatcca ggcccgcaac cagctcgaac tggtcaacaa
      901 ggagctccag gacaagatcc atgagcaggg acagctcttc ggcatctccc gggaattgga
      961 tgaagtccgc tctagtgaag acctttaccc gcgcatcgtg gaggtcgctt cccgtgtgac
     1021 ttcgtccaaa cagatcgggt tcttcatcat gcccccggag cagaacagcc tgatgctcgt
     1081 ttcggagcac ggcctggata ggctgcatga cggaagaagg ctcgtccagg tgagagggga
     1141 gcagctcaaa gaagcgctga accgggagag cccggtcagg atcaaccttt cggacctttt
     1201 tggcgggacg gactccgaaa cgtggcttcg aaattcgagc aggctctctt gctggcccct
     1261 gcacattcga ggggagcttt tcgggttcct ggcggccgcc gccaacggcg aggaaaaggt
     1321 gttcagccaa accgaaatca agctcctgga tttcctcgta aagaaggccg ccctgaccat
     1381 cgagaacatg gccctctatg agagcatgat cggcaacttc tacggcatcc tcaagtcctt
     1441 ggtcaacgcc ctggaagcca aggacctata cacgggaaaa cattccgaaa gagtcacccg
     1501 gtacgccctt cgcatcgccc aaaggctggg atgttccccc gcccagatcg aggccctgca
     1561 gacagtgggc tacctccacg acatcggcaa gatcggcatt gccgacagca ttctcaacaa
     1621 acccaccgtc ctcacgtccc aggaatacga gctcgtgaag aagcacccgg tcatcggaga
     1681 ctccatcgtc tcggaactgg gcctgagccc cgaggaaagg gggatcatcc gtcatcatca
     1741 cgagcgatgg gacgggaccg gctacccgga cggcctggcc cgagaggaga ttcctcatct
     1801 ggcgcggatc gtcatggtgg ccgacgcctt cgatgccatg acctccaagc gagcctaccg
     1861 ggacgccatg tccccaaagg acgccctgca ggagctgcaa agaaacaagg gaaagcaatt
     1921 cgatgcagac gtggtggatg ccttccgtga agccggttcc gcctgcgatg cggaatgatc
     1981 ggcccccatc cgccaactcc ctcccaccct ctccggagat cccatgaaat ccgaaccgac
     2041 cacggatggc gatgctcctc tccaaaagct ctttgaacag tccctggagg ccttgttcga
     2101 aaaagatcgt ttgagccatc tgggaagact ggtacgaggg cttatccaca atgtgaacgg
     2161 cccgctgcag aacgtgtcca tgctcgcgga aatgctcctc aaggggcagg accagctgga
     2221 ccggatggcc cccacgaaac ctctcgaagc cctcggcgaa tgggagaaag ccctggtcaa
     2281 gcagaggcag cgcatggaac gattgtccca gcagatcgcc aacctcgccg ggatcctcag
     2341 ggactttatg gtcctgctcg aaatcgaacg aaacgaatcc gaggtcgacc tgagactcct
     2401 tctatgcaaa ctcgtgcggg ttcttcagtc ggacctcttt ctgaaacacg aagtggaggt
     2461 ggagctgaac ctcgccgaag agctccccct ggtccggatc ctggggcgcg acctggtccc
     2521 ctccctcatg cacctgttcc acaactgcct gtgcgccttg aaacacggta cccggaaaca
     2581 actgacggtg gactgcttcc ttgaaggaga cctcatcaag attctcttcc gggacaccgg
     2641 gtgcggggta gatcctcgga aggtccaagg tccttatttc gatctgttct tctccgactg
     2701 gcccccccct tccgccgcca acgaaaacga cgacaagcat ttgggattcg gccttttttg
     2761 tgttcgcagg ctcctgggtc cctacggcgt ctccatccga atggaaccgc aagatccggg
     2821 gaccctggtc gaggtgttga ttcccgttca agcctgaccc ttcgctccgg agattcgccg
     2881 ggaactaggc ttcgaatagc agcttttgcc caattccgga aaaattttcc tcctccctct
     2941 cttctcgaac cctcgaaccc gacggactcc gtacccatct cccagcaaga gccatgcatg
     3001 acggaaaaga accatttcga tccggttttg aattccaatg gtatatcgca tatttagatc
     3061 catgctctct ctttgttcgg ggccgactcc ttcccatccg ccccccaaaa gagggacggg
     3121 agaaaacctg gcagagttct tgctccgttc cgaatcagcg gttgctccga atcgcttctc
     3181 ccggggcggg cgtgaagccc gccccatggg atggggatga ccacccccga gggacacctc
     3241 cagggggaga agacgcaaat gagaccgagg tggttggagg tcctttcatg aaaatccttg
     3301 agacccagcg aaacgagagc atggagaaaa cccgggaagc ttcccggccc agccggcccg
     3361 gtccatcggg ggcctttcag gagcttctca acacggaggt gggggatggt cctcgaacca
     3421 ccgatccttc cccgcccggt gtatcgttga ccggcctgga ctccctgaac gccatgtctc
     3481 ttctgactcc catttccggg acctgcccca aggatccggc cgagatcgca ctgtccgtgg
     3541 gccggaatct cgatcggttg gcccgggaac tcgagggacc cggatcgagc gagcccaaga
     3601 ggacggaagc cttgatcgag tctttgcgca gcgaagcgga gcgcctccgg gaggagaccc
     3661 ggtcgctgcc tgaagaccat cccttgcgca aaatcagcga cgagctcagc gtgacggcct
     3721 acgtggaatc catcaagtgg aaccggggag attacctcta accgggaaaa gaggaagaac
     3781 cttcggatgg ggccccccag caggcccccc ccgcggggga gtgcccgcag cgaccgcttc
     3841 agggtcgaga atcggcaggg gggcggcgaa ctttcatggg ctgcaatcca tccgggaacc
     3901 catattgaac ccgggaaacg agccccgatg aaccgaggac gacagggggg cggtctgaag
     3961 cacaaaggga ccgaggttcg gagcccccac agtgcccggg aactccctta tgggcagaac
     4021 cgggtatccg ttttcgcagt ttctttgccg ggtgtggagt cgtgaatgac ggggtgtgat
     4081 cgtgaaacct gtcggtcctt gagccgttgg cctggaagct ttccagggct cttgggatgc
     4141 tttgccctag tcttcttttc cctggcgatc ttttgggcac cccccatcga ggcttcagtg
     4201 caggaaagct cagacggact ccgggctcgt ccgggccctt gggcgcactt tcccccgagg
     4261 acttttctcc cacagcgtgc ggaatttacg gaggacccga acgcgcgaac gtatcgaacc
     4321 gcacctgcgg accccagcgt ccaggacttg aacgaagagg agagacagaa agaggaaaag
     4381 gcctggatca tgctccagaa catggacctg tatccccggg aacgccatcc cagaccccat
     4441 ccccccgtca acccaccatc gcacggacct tcggtcaatc gaccctagcc tcccgacagc
     4501 cgcctgcctc gataggtgcc catgggcgct gggatcgaag ctcctccggt ttcgggcgtt
     4561 tcttctgatc ccgccttcca gggccccttt ttggtgttga acttgcatca ttcctcaagt
     4621 gaagccccac gaatgccgat taatgggtcg gatccggccg catgcttcat gggccggggc
     4681 gcgcggtatc tgcgtgccga ccctacccga ggcgtcaacc tggaaccgga gcagaacgaa
     4741 gagagcaaca tgaatttgac ggatgagctc tttcagcgat acagcgatct ggtgtatcaa
     4801 gaatgcggca tcaatctcca tgaagggaaa aaagcccttc tccaggcaag attgaacaag
     4861 agactccgcg caaccggttt tgcatcctat gagcagtact acgaacacat cacctccccg
     4921 gggaattccg gggagtttat ccatttcctg gattccatct ccacgaacct gacctatttt
     4981 tttcgcgaat cccaacactt cgactttctg aacaagagac ttcccgaact gcttgaaacc
     5041 aagataaaag agaagaaccc gaagctgcga atctggagcg cgggctgctc cacgggcgaa
     5101 gagccttaca gcatcgccat gtgcgtgatt ccccacctgg agggattgcc gcgcatcgat
     5161 tttcgaatcc tggccacgga catttccacc catgcgctga atacggccat gagggggatt
     5221 tattccgaag aaaaggccgg aaaagtcccc gaggcttttc gctctcagca cttccggacc
     5281 ctaaagaacc acaacggaag gcgggaattc gaggtcgcct cggccctccg cgatcggatt
     5341 accttcgggc gtttgaatct caaggatcct ttccctttca agggtctttt cgatttcatt
     5401 ttttgccgaa acgtcatgat ctacttcgac aagaagaccc aggagatgat tgttcataag
     5461 ctggcccggt acttgagccc cgggggatac ctcctcgtgg ggcactccga gagtttgatg
     5521 ggcttgtcgc attccctccg atacgtgtgc ccggcagtgt acagaaagta ggacttcgac
     5581 ctgagaaaag gcagagacgg taaagatgag aagggtcgtt ggcgtcgcgg acatggcggt
     5641 cagtaacaat cctgccgaga acctggtaac ctactccctc ggctcttgca ttgcggtggt
     5701 cctgtacgac gctacggtca ccgtgggggc catgctgcat tacatgctcc cggactcgaa
     5761 tctggacacc gagaaggccg ggagggtccc ggccatgttt gcggatacgg gcattccgtt
     5821 acttttccga caatcctacc aatacggggc caccaagaag aacctggtgg tgaaagtggt
     5881 gggaggggct caaatcctgg atgagaacgg ccttttcaac atcggcaaac gcaattacct
     5941 ggctctgcgg aaaatctttt ggaaaaacaa cgtcttaatc aaagctgagc atgtgggcgg
     6001 caatgtgaat cgcaccgtcc gactggatat gaagaccggc caagtgttcc taaaggtttc
     6061 cgggattggg gaattcgaac tctaagaacg ggttagagca cacgggacaa acgaccatgc
     6121 cgtacaatgt tctgatcgtg gatgattcga gatcgatgag agaagtcatc aagaaggtct
     6181 tgcgactttc cggatttcaa attggggact gcctggaagc cggaaacggg gaagaggcct
     6241 tgagcgtcct cgcaaaccat tgggtcgacg tgattctttc ggacatccac atgccggtga
     6301 tggatggttt tggattgctt caagccctaa cggcccagga agatctccgg gacgtaccgg
     6361 tggtgctcat caccacggaa tccagccact cgcgcctgaa ggaggctctg gccctcggcg
     6421 cgaggggata cctgcgaaag ccttttcgac cggaggaggt ccgagccttg ctggcgcaaa
     6481 tcatggggga aacggatgga tcccaagcgg gaaatgttga tcagggatgc gatttctgag
     6541 gtgttggaag cgatgttctt tgtttccgtg ggcttctcgg aacctgcgca cgtatccgct
     6601 tcctctctct tctgctgttc gtccgtccaa atgtgccggg gatccgaaac catggagatc
     6661 ggcattgcgg tgacggaatc cttcggcagg atgatcgccg ccaatttcct ggggatccgg
     6721 gaagaggggg tgaaatccga agaactggag gacgttctca aggaactggt caacatggcg
     6781 gggggcaatt acctcgatcg cgccgacagt gaaaactggc aactggaaat cccgaggttc
     6841 cggaggtttg cgcaatcctc gagcgcgacc ctatggggca tgcccatgga tgtcctggga
     6901 gaacccgcag ggttggtgtt catcgaatcg ggatctttct gttctcaatc gtcttgatcc
     6961 ggccggggag catcaggtgg aaaatacggg acctctttcc catcaagaat actttcttcg
     7021 tccgggctac atctttgtta cccagcgccc cactctcatt tccacggtcc tgggaacctc
     7081 cgttgcggtt gccctgtggg atcgagagaa gaagtggggt ggaatgaacc attttctcta
     7141 tccgctggtg ccggaccccc acaaggccac ggcccagttc ggcaacgtcg ccgtgagggc
     7201 cctctacaac ctgcttgtga gggaaggcag ccacaggagc gatcttgacg cccagatttt
     7261 cggcggagca cacttggcgg aagcttcccc cgaagagatc gaggtgagcc gtcgcaacat
     7321 caccgtcgga cgcgaggtcc ttcggaaaat cggcatcccc atcatttccg aagatacggg
     7381 cggcacaagg ggaaggaaac tggtcttcaa caccgccacc aatgaggttg ccgtatttcg
     7441 agcggagcgg ctgcgcgaca gtgattggta tccataccgg gacaaacgct gagagatttc
     7501 aatgcagaag gcttccaatc caatcaaggt cctgatcgtg gacgactcgg ccatcgtgag
     7561 gaagatcttc acccaggagt tgtccaagca cgaagacatc gaggttgtgg gaacggcacc
     7621 ggatccctac gtggcaaggg acaagatcgt gaagttgagg cccgatgtgg tcaccttgga
     7681 cattgaaatg ccccgtatgg atgggatcag tttcctccgc aagctcatga agtactttcc
     7741 cattccgacc attatcgtca gctccttgac gcccaagaac agcgaaatgg cgctggaagc
     7801 catggaatcc ggggcggtgg aagtcctggc caagccggga ggctcctatt ccgtgggcga
     7861 catgagcatt cagctggcgg aaaagattcg ggcggcggca caggtaaggg tccgatccga
     7921 tttgggaacc ggcccgggag cgagatcccc ggaacccgtc gaagagaacg cctccctttc
     7981 ccgggccctc aagcgcacga ctcacaaggt cattgccatt ggggcgtcta cgggaggcac
     8041 cgaagccctc aaggtggtgc tcacgcaact gccctccaat acccccggga tcgccgtggt
     8101 gcagcatatg cctccccggt ttaccactgc ctttgccaac cgtctgaacg atctttgcca
     8161 gatcgaggtg cgtgaagcca aggacgggga ttcggtgctt cccggcctgg cccttatcgc
     8221 cccgggcaac taccacatgg tgcttcgcag aagcggagcc cggtactacg tggaggtgaa
     8281 aaccggtccc ctggtgtgtc accagagacc gtcggtggat gtgcttttcc attccgtggc
     8341 ccagtacgcg ggagccaacg ccgtgggggt gatcctgacc ggaatgggaa gagacggagc
     8401 cggcgggatg ttggagatga agaaggccgg ggccccgacc atcgcccagg acgaggcgag
     8461 ttgcgtcgta ttcgggatgc ccaaggaagc catccgaatg gggggggtgc agcaggtggt
     8521 ctctctgcag cagatccctc atacggtcct gaaggccgtg gcctcggacg gataaaaccc
     8581 ccgggaacga gtcctccgga gccggcccct gcggtggtcg gcagggctca atcctccgcc
     8641 gtggggcgga caagaacccc cgggagagca acccgccgta tcacacggtg tcaccggtga
     8701 tcgccacggt gtgagtcaat taccaatgga atgttatggg taaagaggag tttagcgatg
     8761 gcctacaagg acatgaccgc cctggtggtg gatgacttcg caaccatgcg ccgcattgtg
     8821 cggaacatct tgcgggactt ggagttcaaa aacatcctgg aagccgagga cggaacggcg
     8881 gccctggaag tgctcaaaac caacaaagtc gacctgatcg tctcggactg gaacatgccc
     8941 aaaatgaacg ggttggactt cctcaagcaa gtgcgggccg ccgaacagac caaagacatc
     9001 ccgttcttga tggtgactgc ggaagctcag aaggaaaaca tcatcgaagc ggtgaaggcg
     9061 aaggtgagca actatatcgt caaacccttt acggccgcga ctttggggga aaagctggca
     9121 aagattctcc ccaacgatta gcccgaaccc ggtctcgagg gctcgaagag aggataggac
     9181 tccgatgcaa gacgtggtgg agaaactgac aaacggagtg atcgatggtc ctgaggatct
     9241 catgggcatg ggggagtgcc tcaatctctt ggaacagctc gaggcagcct cttacttttc
     9301 cggagaggcc gccgacctct tgccgcgaat ccggcagctg ttgaaccgga tgatcctgga
     9361 agaatccccc gacctgcaga aggactggca gacggttcag ggtctgatcc gggagctctg
     9421 ccccacggga aacggcggac cggaatcctc cgaggattcc gtgtcccact cggacctgga
     9481 ggcgatcaag gacctcatcc gggagccccc tgccggccga acggaccccg tgggtcccct
     9541 ccccgcgacc tccgagaccg ccggggaatg ggaaggcgga tcggagggtg ggagccgagc
     9601 acccgagggg tcgacggcgg aaatggagct gcaggacccg gagctggtta gggattttat
     9661 cgaggaagcc aaggagcatc tctcctccat cgaattgaac atcctggccc tggaaacgga
     9721 ccccaaggac caggacgcca tcaacgccat tttcaggccc tttcacagca tcaagggagt
     9781 tgccgggttt ctgaacctca aagacatcca tcatttgagc cacgaggtgg aaaacctcct
     9841 ggactgcgct cgggcgggca agatcctcat caccgacgac gccatcgacc tgattctcaa
     9901 gtcggtggat ctcttgaaga ccctcatcgg ggtccttgag gattccgtgg cccaggggga
     9961 accccttccc gacaacgcgg aagtcgtcga ggattttctc caacgattga gcgcgttcca
    10021 agataccccg gtggcggagg aagccccggt gcgcaagctg ggcgagatac tcgtggaccg
    10081 gggagtcgtt gaagacgaaa ccatccatcg ggccctggag gacagcagag cctccgggaa
    10141 gaagctggga gagctgctca tcggcaaagg gatggtcagc agccgggagg tgtcctcggc
    10201 cctgcgggag cagcgtcggt ccaaggaaag cgccgcttcc gtccgaatcg acacccacaa
    10261 gctggacaac ctggtggaca tggtgggaga gctggtcatc gcccagtcca tggtccttca
    10321 gaacagcgag gtggaagcca tcaaggagca gaagttccag aaggacaccg tccagcttcg
    10381 ccggatcacc aacgagctcc agcggatttc catggccatg cggatggtgc ccatcaaaag
    10441 cacctttcag aagatgatcc ggctggtgcg ggatttgtcc cgaaaatccg ggaaggaagt
    10501 caccctggaa atgaaggggg aagagacgga aattgatcgc aacatggtgg aagagatcta
    10561 cgagcccctg gtgcacatga tccgcaactc catcgaccat gggatcgaat ctccggagga
    10621 gcgaaaagcc gcaggaaagg tccttccggg ggtgatcgtc atctccgccg agcagaaggg
    10681 cggtaacatc gtcatcgaaa tcgaggacga cggacgcggc ctgaatgccg aaaagatccg
    10741 ttccacggcc gttcaaaggg gggtgatttc ccccgacgaa cccctggacg aacgggggat
    10801 ctacgatctc atttttcatc cgggcttctc gaccaaggac aagatcaccg aggtgtccgg
    10861 ccggggcgtg ggcatggacg tggtgaaaaa gagcgtggaa cgtctccgag gcaagatcga
    10921 aatctccagc catcgaggtc ggggaacccg gtttcagctg aaactcccac tgaccatggc
    10981 catcatagac ggcatgatca tccaggtggg gggccagcgc tacgtggtgc cgaccatctc
    11041 catgaaggaa tccctgcgac cgggccggga ggcttatcag acggttcacc gaaaggggga
    11101 agccattaag gtccgggaca ccctgatgcc tctcatacgc ctgcaccagc ttttcggcgt
    11161 cgagcccaag taccgggacc cctgggaagg cctgctcctg gtcgtcaacg aggacaaccg
    11221 ctcctattgc ctccaggcgg acgaaatcct gggccgacag gaagtcgtga tcaagagcct
    11281 gggatcgtcc ctgaggcacg tgacggggat ctcgggcggg gccattctcg gggacgggag
    11341 ggtcgcactg atccttgatg tcaaagggat cgtgagccat tacgagggaa attcgagatg
    11401 acgatggaac tgcacgaaga agattgggct gagttggtac agatcctcga gacggtggag
    11461 gaacacctgg gtttcctaga cggccgcccc gacgatcaag agaggctgga cacctgccgc
    11521 cgggctctca aatcctttca caccaccgcg gccatgctgg ggctgggaac tctcgagaag
    11581 gcgggaatgg gcctcgaaaa agccctcgcg gaccagcgga ccccgctccg ccaacggccg
    11641 gacgacctgt ataccgtcgg ttccgccttg aacggcatta tcgaggaact gaagaaggcg
    11701 gccgacggcg gcgagatctc ggcagttcga gtggaggaga tcttgacggc cctggatggg
    11761 gcccaagaac cgggtgaaac gcccccgggt caagtccgag aagaggaaga ccggcacccc
    11821 gaggggggat cctcggcccg gaagcccgtg ggaaccatcc cgggaaagcc cgccgacgct
    11881 ccggcagtgg atttttccgg gctcgagcag gtcgtggctc acctgggagg gcacattctt
    11941 tacaagagca acgggagcaa cagccccgca ttccagctcc atttcgaagc ataccccgcc
    12001 gttgtggaac agttgacggc cctgctctct cccattcacc cggagggatc cttggctccc
    12061 gagctcagcc gggaagaccg gaggatggaa aagatcctcc tcatcatcaa ggatttcatg
    12121 aaagcctttt ccgagggcaa cctcaagcgg gcccaggaag cacttctttc cctgaccgac
    12181 caggacaagc acgtcgccct ctacaacgag atcggcacca tggcccgcca actccacagc
    12241 tccctcacag gcttcacatc caccctggac cccgccttga aggaaatggt ggaagacaag
    12301 ctccccgatt cgggcaaccg cctcgaacac atcctcgaga tcaccgagaa ggcggccaat
    12361 accacccttg accacgtgga agccattcag aagaggaacg aaagagacct ggaactggtg
    12421 gcccaaatgc aggacatggt gaggggactg caagccatcg gagaccgggc ggaaaggagg
    12481 ctcgaggagg gctcggccat gaccgagaag ctatccgcct cgattctcca aacccgggac
    12541 gacctgacca ccatcctcac ggcccaggat taccatgacc tgacgggaca ggtggtcatg
    12601 aagatcctcc acctgctcaa ggacctggaa atgaagctgg taaacgtgat tcaagctttc
    12661 ggagtgccaa tggaaggaag aaggaagggt ccgtccgacg aactctacgg cccggcccac
    12721 aagaagagaa ccgaagccct ccactcccag gacgacgtgg acgccctgct ggcggacttc
    12781 ggattctaaa cctcaaagcc ctgctgcctc aaaccgcaag ggcagcaggg ccggtgctgg
    12841 aagtccgggc cgcggaatcc gccgttaccg cccggggggg aggaagacgc aaggcacggc
    12901 gggaagaaac ccctataccg ccttgtccag caggtccaac tccccggcag tcaatactcg
    12961 atcgatatcc aacaaaatct tgacgccccc gttcatcttg gccatgccca ggatgtaatc
    13021 ggtattcaac ttggtgccga aggtgggcgt ttcctcgatg tcctcgtttt tgatgttcat
    13081 cacttccgaa accgaatcca caacgatccc catcatgatc gatccggaat cactggcaat
    13141 ttcgaccaca atgatgcagg tgcgctccgt gtagtcggtc gcatccatgc tgaacttgct
    13201 ccggaggtcc accaccggga tgaccttccc ccgtaggttg atgactccct taacgtagtg
    13261 gggagtttgc ggaatgaggg tgatgggcat catgccgatg atctccttga ccttcaggat
    13321 cccgatgccg tattcctctt cggcaagggt gaacgtcagg tacttgccgc ctttctccgc
    13381 cgaggtccgc ttcgccttat caagcgttct agccagctgt tccatggaaa tgcgtctcct
    13441 ttctaccggg ctggttggtt cttgtcgtgt tcacaatcat atgaatcggg aagtcggtat
    13501 aaaaacttga gatctttcca acttttttgc gttcgagccc ccatcgagga cctatgcaag
    13561 ttctatggca agtcctctcg aggatcggag aacgttcttc cttcctcttt tttttctaaa
    13621 gttctctccc ggaccatccg acaagtaggt tgaaggagaa cccagccatg gtaatcacgg
    13681 actatcgcat ccaaagcgtg cttcgcactt acagcaagca gcttcagcga tccaagatta
    13741 cgaagaggct cgagtccgaa gaccccaaac ggccccagga aaaggtgacc atttcggagg
    13801 aagctcggcg ccgtgccatc gtggaacgag tcacatccca ggccatggag cagatctatc
    13861 agcagagtga agatttcgat ctgctcaaag gctaggggtc accggcgtcc cggcgcgatg
    13921 atgcccctcc ccccactgcc gcaagcggca tgcaatgcag aaggaaaccg ctatggatgt
    13981 caagaaggta caagcataca tcacccaggc tccactgaag accgccgagc cgggttcccg
    14041 aaccccggga gaggaaaagg cgcaggcgag ctccgtggaa gccgacagcg tgaagctctc
    14101 caagggatat cttgaagtgg ctcaggcaaa gaaactggtc atggaccggg ccgaagaaat
    14161 tcggatcgac aaggtcgagg ccattcgaag ctccgtcgat aacaagacct acgcgatcga
    14221 tgcggagaag atcgctcaga agatgacgga ggaattatgg tggtagagag agccgccctt
    14281 tcgcccccgg gttccctgca gaggcccggg gtccgtcacg gtcgcgggat tcccggctcc
    14341 gccccacgtc ccgtggaaaa ccgccccctt gcccggccca gccgggatta gccggttacc
    14401 cccgccttcg cccgtcccct ttctcctttg atttccgccc gtcttttcgg aactacagga
    14461 atttggccag gttgaggtcc ttgatcaggg tcgtcgcacg cagggcggct tcataggcca
    14521 cctgtttttg ctggagtcgc gtgatcactt cggccacatc cgcgtcctcg atctccgaga
    14581 gtcgatccgt gcggttcacg gcaatgcttt ccagggccga tttttgtcgg tccagtcctt
    14641 ccagccggac tcccaccgtg gactgcttca tggtaatgcg ctcgtagtcc tcgtcgagac
    14701 tacccaacag ggaggtgatt ttgtccgtgt ccccttcccg cacggcatcc cgcagatcca
    14761 tgatcctctt gagcaggttg gtgtccacat cggacgcatc cgcaaagaag acgtctgctc
    14821 cgttcaagtt gatggtctgt cggaagtcct tccccacgcg cacctggtag tcctgggtgt
    14881 ctccgtgata ggtgaaatcg ggatagccgg gatccgtggg gtctcccagt tggaaagggg
    14941 cggtggacgt ttgttttcca ctgaagaggt agagctcccc gtactgctcg ttcatgaccc
    15001 ccagcgcctc ctcgaagatc tgctgcagct cgtccacctt cgcggaccgc tcctcggcat
    15061 ctaccagctt gaccgcgccc atccccactt ccttggcacg catcagcagg tcggaagccc
    15121 ggttcaaagc atcctccacg gcctggttcc aaccgatccc gaagtccaca ttgtccttga
    15181 aggtctgcaa ctcccgcagg ccctgcttca tctcgacctt ttgagaccag gcggcgggat
    15241 cgtccgaggg ttcgagcagt cgtttgccgg acccgatttc cgtggtgagt cggtccaagt
    15301 cgacgctgtc ccggttgatg ttgagcaaca tcctgtccca agcggttgca aagctgattc
    15361 gcatgacccc ggacccccta tcctaccatt tcgatggcgg tgtcgagcat ttccgcagcg
    15421 tggctgatca ccttcgccgc agcctggtag atttgctgaa acttgatcag gtccaccatt
    15481 tcctcgtcga tgctgactcc cgacacggac tgctgctctt cctgcaaccg gttcaacaaa
    15541 gtcgcctgga aatccgccca ggacaaggag tcctgccggt ctaagcccac ctgctcctgg
    15601 atgcggccca ggtactcgag aaacgtggca tttcccaggt tggattcccc cggaagcccg
    15661 gagaaccggt gggactgcaa atccgcaatc tccagcgcct gggcggaatt caccacgaag
    15721 ttcgcatcca cggccaggtc gcttgctttg aagtcctcac ccagcaccga atcgtcgaat
    15781 acgacgctgc ctcccccctg ccggtgaatc gcgttcactt ccgtaatgag ggcggcggca
    15841 aaggcgttca ggcgatccag gtaggtgagt tcggcagcat cgggattggc ctgcacaaag
    15901 gagagatcgt gctggttgcc cgcaaggttg tagacgctga gcaaccccgc cagtttgccg
    15961 ccggaaagct cgttttgtag ggtcggatcg tcgggcaccg cggttccctg gtagtcccgg
    16021 taggtgaccc gatggtttgc gtcgtcgtag accaagctcc cgaagtcgcc ggcggaaacc
    16081 agggtgatgt cggacccgta atccgtcaca cgaagggtga gggtcccgtt gtcttcttcg
    16141 tcccaccgga cgggaatcag ctccgccagg tcctgcacct tctcatagcg aaggtccctc
    16201 agatcatttg cgggctgccc ggtggactcg gccttcatga tttgctcgtt gtaggcggca
    16261 accgcttcca ggagtccgtt gaccttggtc accccgctct tcgcctcgct ctcgatcccg
    16321 accgcgatct ccgtcatgcc ccggtatgcg tcccgaaccc ggtcaaccag gctttggacg
    16381 gcttcttcca ccaaggcttt ctgggaaatc ccgtcgggat tccggttcag agcctcccag
    16441 gcatcccaaa aggctcccag ggcctggctt aacccttgct ctccgtcatc ggcccaagtc
    16501 gcctcgatga gggacagttg ggtggaccgg gtgcggtaat ccgcttcttt ggagacggcc
    16561 gccgtcaggc gacgctcgat aaactcattg cggacctgga ccacggaacg gattcgagcc
    16621 ccggccccca gccaggtatc ccggacctgg taaggcgtgc tcgtctcctg ggccaccctt
    16681 tgccttgcgt aatccttgct ctcggcgttg gcaatgttgt gggaagccgt ctgcacgcac
    16741 acctggcagg tgagaagggt gcgcttggcg gtttccaggg tcaccatgag tccggacatc
    16801 ctaagcctcc ttcctcaacg ccaatccctt gacggcaatt gcggcggcct gggggcctcc
    16861 cccccggccg ggcaaatagg ttgaaggacg aaggcacttg agcaggtcct gcaggtagag
    16921 gagagaaccc tctacgaaga cttggttcga gcgattcttc tgcttgatct ccttcaaccg
    16981 ctccgccagc cgcttatagg ccgggctgtc ggccgccgct ccgtttagag ccttcaagcg
    17041 tgccaactcc cccacgctct ctcccagttc cagggtaagg gcttcctttc gggacaggag
    17101 ggggaggagt tgctccgttt gaaaggctct cagcacggaa gactcttcct ccagcacttg
    17161 cagcagttgc tccgccttga gagagcaaac tcccaggagc tctccctgga ggggggagca
    17221 ctctccctct gctgggaccc gtttgtcggc ttcccgaggt ctcatgagcg gatccttcct
    17281 tccgagttcg tttgcataag gattctatcg gtcggatcga ggcattgctt taacgaaccg
    17341 gcccaaacgt tgcggtccgc ctctcaagga tttcggaccg gattctgcgg tttctcacct
    17401 gcctcgacca ggggcaggag ttgccgatac atgagctcgg ccaatccgca cccgggaccc
    17461 cggctcagtt cccgggcaag gctttcgtcc ctcaggctct cgtatacctg tcttgcctgg
    17521 tccggttcct ccgcccgctg aatgccctcg cgcatgctct tgaggacgta gttggcaaag
    17581 atggcctcga actcccgaca ggcttccttc aatcgcttct tcttgagctc cgggtccatg
    17641 ggagcaactc ccaactcggg gctcaccccc ggcaacatgg cgcgtttccc tcctaaatga
    17701 tctccagctc cgcctggagg gcccctgcag ccttgatggt ctggagaatg ttgatgaggt
    17761 cccggggact cgcaccgatg gcgttcagac ccttcaccac ctggccgatg gtgactcccc
    17821 ccaccatgga aagggcgccc ttgccttcct gcacttcgat acgggtctgg ggcgtgacgg
    17881 tggtctcccc ccggctaaag ggcaggggct gggagaccgc ctgctgttcg gtaatctgga
    17941 ccgtcaggct gccgtgggcc accgccaccg gatgaatctt gacgctctct cccatcacga
    18001 tggtgccggt ccgttcgttc accacaatct tggccgtaat ctcctgttga atctcgaggt
    18061 tctcgacctg ggtgatcatt tccggaaccc ggccctggaa ggaggcgggg accgtgagct
    18121 tgatggtggc ggcgtccacc gcctgggcgt tggcgcccgg cacgagagag ttgaccaggg
    18181 acatggcgcg cgtggccgtg gtaaagtccg gtttcttcaa gatcaagtcc agctgctgga
    18241 cgttggtgaa atggacctgg acctcctgtt ccacggtggc ccccccgctg acgaaaccca
    18301 cggtgggatg gttcttttgc accgtgcttc cgctctgccc cgagacgctg aaacccccga
    18361 cggagatggg gccctgggcc agagcgtagg ttttggcgtc gggtccctgg aggggggtca
    18421 aaaggagggt tcctccctcg aggctcttgg catccccaat ggaggacacc tggatgtcga
    18481 tgcgggtgcc cactcgagcg aacgggggaa gcttggccgt caccatgacg gcggccacgt
    18541 ttttcacctt gatctgggtc gggtccactc gaatgcccac gttgtccagc aggttggcaa
    18601 gggtgttcac ggtgaacatg gtcttgtcct tgtcgcccgt gccgttgagc cccaccacca
    18661 atccgtaccc gatgaggggg ttggaggtgg tcccgcgaaa ataggcgagg tccttgatgc
    18721 gcgccgagtg gccgaccgat gccagaaaca ggacggtcag caggagcgcg gccggaagtg
    18781 ttcgggtccc ggttcgtccc atgagcacct cgttagaaag gggagataag gtccagcaat
    18841 tggaaggccc aacccggttt ctgatgctgt gtgacggggc ctttccccac gaagaagatc
    18901 tttgcttcgg cgatgttttg ggaaaggacc gtgttgtgcc gagtaatgtc gttgggccga
    18961 atgatgcctt ccaagacgat ctgctgcatt tcatcgttta ccttcgtcca ccgggaccct
    19021 cgaatcagga ggtttcctcc cgggaggacg tccacgaccg tcgccgtcat gtaggcggtc
    19081 atggagtccg atctcgtggt ggcgccggcg gccgtaaatc ccgtcccgaa ctgcccggag
    19141 tacgggccga aagtggcgcc gggaaccccg ccccccccgg cggcaccggt accgcccccg
    19201 gaggtcagcc cggctccccc gaaggtaaac gttcccgaaa ggtccttgct gcgcagagcc
    19261 ttagtggttg cttccttgga ccccttggct tcttcactca cggtaatggt cacgatatcg
    19321 cccactttcc tcgccttgag gtcctgaaaa agggaaccgc tgccccccgc ggcccacaag
    19381 gatccggtgg gagcctccgg ggcgtcgccg ggggttggtg gaggaatggg cgtcatgggc
    19441 acggggcttc ggaccactgg gccggactgg gggggagaag gttgggtggt ggcgcaggaa
    19501 accagaaaaa gcccggagat caccaggggg aacacggcca gccgccccgg ggagaacctc
    19561 ccccttttcc tttcctcggg atgagaagaa tcgtttgctt gcgggtgcat ggctctactc
    19621 tcgctgtttt gtaagatgcc cgaccggaat ctcgacacgg cacgggatgt gggccccccg
    19681 ccgacgggcg ggacggggaa ggcccccttc gaccctgcgg cccccggccg ccctagggaa
    19741 tgacgcgtac cgtcgtgggg tccaggaccc ggcagtggac cgtgcggttg gtcatgacgt
    19801 tgatcactct cacggtatcc cccaccccgg catcttcccg cacctgaccc ttggcggtga
    19861 gtctcaagcc gggcaagtca tagagcatgg tgacggcagc cccccgcttg agggccggag
    19921 gattgtccag gtcggccaaa ggaacggcct ggcgcaaccc cacgtctcga agcacccgtt
    19981 tccccaccac ctggtcgatc ttggtggcga aacggtccgg ggattcggag atgttcatct
    20041 tctgcagttc cacgtccttg gccgacacga cgtcgttccg tttgaggggc tgggaggtga
    20101 ccaccacgtc ctggaacaca tccaccttcc cgctcacccg cagcgatcga tccttctcgt
    20161 ggttgacgga aaactggatg gtgaccgcca cgttccccat gaaccgctcc tggggatggg
    20221 atgtcacttc gtaggaaagc tcccctgcag gcaggtccac cggccccgaa tactggattc
    20281 cccgtatgac cacgtcctgg gggctccacg gacccctcga gagcacatag tcccggtaga
    20341 ttccctcgat ctgttcccgg gatacctgca tggattcccg ccggatgaga atcctgtcgg
    20401 gaatggaaag ctcccattga gccgggtcct ttccccggga cgacagaaca cggtggacga
    20461 agctcttgag ctgaggggcc tcgatgtact tctcggtgcc tggagacggg gcttctccga
    20521 ggtcgatgga agacagcagg gccatccaat ctcccgggat tccttccggg ttgcacaggt
    20581 cccacagagt cacccgtttg cggtgaacca ggaccgagga ggaaaactcg atcttcacgg
    20641 cagaggcccc ggcccaacac accgattcgg gaccggcgca gtccccgggc gggaacggga
    20701 agaagcccga aaatcccgca cccaggagca ggacagccca aaagaaggtc ttggccctca
    20761 tggtccctcc gttcttctta ggattttaca ttgttggcca tctgcagcat gtcgtcggag
    20821 gccttgatga ccttggaatt ggcttcgtag gccctctgcc ccacgatcat gttcaccatc
    20881 tcttccacta cgttgatgtt ggaattctcg aggaagccct ggagcagggt cccaaagccg
    20941 tcgtcccccg gctggcccgt ctcggagtcc cccgaggcct cggtaggcat gcagtagttt
    21001 cggcccaggc tgatcatgcc tgccggattg ggaaaatcga agaggttcag ccgctgggtg
    21061 accagggctt ttccttcctg gtcgacggcg gtgaggtttc ccccggcatc gatgttgatg
    21121 gccacggtgc ccttgggaat gctgatctcc ggctgcagcc ggtttccttc ggcgtcgcaa
    21181 atgtagccgt cctggtccat tttgaaggca ccggcccgcg tgtacacttc atcggtgccg
    21241 cgcaagacct tgaagaaccc ccgaccctcc acggccaggt ccaactggtt ccccgtctgg
    21301 gtgaagctcc cctgggtgtg gattttctga acggagacgg ttttggcccc catccccatt
    21361 tgcaccccgt tgagaagacg ggtgttgttg gacgtttccg ctccgggagg ggtcaaattc
    21421 tggtacatga ggtcctcgaa attcgcgcga ctcttcttgt agcccgtcgt attggcgttg
    21481 gccaggttgt gggcgatgac gtccatgttc atctgctgag cggccatgcc cgttgcggcc
    21541 gtccataggc tgcgaatcat ctcggtctcc ttctacccgt tggggtttcc caacttgttg
    21601 atgagctgtg cttcctgttg ctcgaacacc tgaatcgtct tctgataggc ttcaaagacc
    21661 ctgctcgatt caatcatccg ggccatttct tccacgagat tgaaattgga ttcctccagg
    21721 gccccctgct ccaccgtgaa ttgctccgca ggcaaagggg cctcgtcctc gacgggtttg
    21781 acatatcccc cccgaacttt ctccagccgt gcctgggggg aaaacttcac cacatccagg
    21841 gtacccacca actcctgctc ctcggtcttg ccccggtcgt caaagatttg accgtttttt
    21901 tcgatgcgga cgttggaatg ttccactcgg atgggaccgt tctggcccag tacgggccag
    21961 ccctgctggg tgacgatcat cttttcggaa ttgagagtca aattacccgc ccgcgtgtag
    22021 agaatcccct cgggcgcttg aactcgaagg aatccctctc ccaccagggc gatgtccagg
    22081 ggatttcccg tgggttgaat ccttccctgg gccatctggg tatgggtgtt ctggtggagg
    22141 gcgtccttga aatgaaccga ttccctttta aagcccgggg tggcggcatt ggcgatgttg
    22201 ttagcgacga cggccatggt cttttcctgg tgcatcgcgg ctatggaggc cccataaatg
    22261 ccgattctca tggctccttc tccttcccac ccccgagagg atgcaatgag ggtgccagat
    22321 gggtctgccg gaaatccgga tgcagggccg atttccggtg cagccttcgc cccttcggtc
    22381 ccccggacct tccccttttt ccccggagtc ggaaaaatct gccgcaaagg ataggagaga
    22441 acggccgggg cggtccgatc gcaggagcgc tcctcggccg gggcgggagc ggggttcgga
    22501 ggcttggacg gttcagcgtt ttcccgtgat ggtctggagc ttcaacacga gctccacttc
    22561 tcccacgggc ttttgcagat gccgggcgat ggaagttgcg tccatcccct tcctggccag
    22621 ctgcaggatt tggtcgttct gggaagcccg gggctggggg ggggacccat ggggaggagg
    22681 caggggccct tggttctgca gggcttcgag cttgaggctc atgagctgag ccctttccag
    22741 ctgctggtcc agcttggcca ggaattgctg gatcaactgc cgacgttcct ggaggttggt
    22801 ttcaaactct gcggcaatcc gttgagtctc ttcgacaatc tttccaaaag accgggtgag
    22861 ttcttcgaca ttccggtctc ttcggaatcg cctgaagaaa aggcaagcca ggaaaaggat
    22921 caggagaacg tccagcagga tctgcaggag ggtgagcgcg tgcggctgat gaaaaaacca
    22981 gtccatgaat cgttccacct cgtgttgcgg cccctcagag aacatccatc tggaggagcc
    23041 ggacggcgcc gatgccggtg gcgggcagac tttccttcag gtgggaaaaa agcttctttt
    23101 ccaccgtctc ctgccagtgg gtgtaggaca acagctccgg gtgctcatgg aggaggtatc
    23161 ggtagatccg gtcccggagc attgtgggag actgttcgaa ctgctcgaag gacctttccc
    23221 ctaggaactc cagttccagt tccagggaaa ggatatccgc cttcttgccg accttgaggg
    23281 gaagcaggag ctgtaccttt ccgggaaagc gggggatggg aacgggcttt cggaccagcc
    23341 tgaccggagc ggaaccgttc gaggtcggcc accacagtgc gcaggcaaat ccaaccatcc
    23401 ccagaagcag gaaagccgcg gctcccagcc acaagatctt tcttttcgga acccttcttc
    23461 tttgctctcc cggatcctct tcttggtcca ccgcggcccc ctccgtttcg agagctgccg
    23521 gggcgggggt ttcttctttg aaggaccggg aaagactctc gtgggaaaaa ccctccaggt
    23581 cgtccaggga gaagtctttg tcggaggcgg ccttgctgtt cccgttcccc cccgaagggc
    23641 cttcgaacaa gctctgcccc cccatgggag agatctggtc gaactcttct tcgatctttt
    23701 cgaggacttt ctcgtcgaat tgggcccctg aaggcacgag ctcctcatca atgttttttc
    23761 tggcgatctc gcggcccatt cttcaatcct cccttgtcac accaccgcct cccggggttc
    23821 caggtattcg ggcagatcct cccggcgcag ctttcgagcc agccgggtgc gcaaacgaag
    23881 aacggccttg gtgtggattt gggaaatgcg cgattcggtg taccccagaa ccgctcccac
    23941 ctctttcatg gtcagctctt cgtagtagta aagggccagg acctgctgct ccttttccga
    24001 gagggaacgg atggcttcgg cgagatgtgc ccgcagttcc ttctggtgaa tcagccggaa
    24061 caattcgtcg gattccgtgg caaggaaggc gctcccccgg ttttcctgga aggcatcatg
    24121 gatgtcctcg ggaaggatgg aaatgccctt gatctcatcc aggagcttga aataggactc
    24181 catggaaatc ccgagctgat cggcaatttc ctcatccgag gcgtctctgc cgagacgttg
    24241 ttcgagggcc aggcaggtcc tttccagttc gctgcttttc tgacgcagag accgagggac
    24301 ccagtccata gaacgaatct catcgagcat ggctccacga atgcggatct tggcataggt
    24361 ttcgaactgg atcccctgac cgggatcgaa cttgtcaatg gcgtcgatga gcccgatgat
    24421 cccggcattg taaaggtcgt ccacctggat gttgttggga agacgggaga ccaatcgcag
    24481 ggccatgtat ttcaccaggt ccgaatgctg gaggatcatg cgttccttgt ccggcacggc
    24541 gggggaactg tcgaacctcg cagtaccgta agcgttccgg gcgggatagg atctctgtgt
    24601 catagggcct tcaacatttc tccggctgtt agacttgcag caggcgtttc caaaagaact
    24661 gcacattccc gttgttctgg tgcgggggac tcttccgaat cctctccgca aggctcaaaa
    24721 aggcccgggc tgcagcactg cggggaaagg catgcaacag gggtctctgc tgcaggatcg
    24781 cccggggaac attgggatcg cggggaatcg acccgatata gtccagggag atcccatcca
    24841 ggaaatgatc cgcaactgtg ctgatcttgg cgaagattcc cttgcccgcc ttttcatcct
    24901 ccacgaagtt ggccagcacc cggaaatggc gctctccgta tcgggtgtag agcaccttga
    24961 tgagggcgta ggcgtccgtg agggaagtgg gctccggggt gatcacaacg attttttcct
    25021 gggcggccag gttgaagtag agcaccgtat cggaaatccc ggcaccggta tccaccagaa
    25081 agatgtcgat ctcggactcg agctcgtcga gcatttccag catgaggagc ttttgttcgt
    25141 ccgtaagccg ggtcaactgt tgcacaccgg agcttgcggg catgatccgg acgcctccgg
    25201 gtccgttgat gatgacttcc ctgagttgtc gtttgccttc gaggacatgg gcgatgtttt
    25261 ccttgggtgt gaggcccagg aggacgtcga tgttggccaa ccccaggtcg gcatccatga
    25321 tgagcacttt cttgcccaac tgagcgaagg ccacggccag gttcaccacg atgctgctct
    25381 tgccgacccc tcctttcccg ctgctcacgc tcaacacccg cacgaggggc ccctgcggat
    25441 cggcaatccg caagctcttt ttgctccagg cgatgggacc cgctttcctc tcgcgcaaac
    25501 ttactgcttg atccatttca ttcctttctt gaagagccat tcccggaggg aatgagaagg
    25561 gaaaggagcc gtttgtgggt ggccgcctcg atgtcctcgg gaacccgctg cccgatcccc
    25621 aggtaggaca caccgtaggg gaaacgaagg agctgattca caatgcatcc atggctcatg
    25681 gtttcatcca gtttggtgaa aatcaaactg tggggatcca aaggccgaaa atgttggatg
    25741 gtctgcttca agtcttcatc cttggccgtg gcattcaata ccaggaagga atgcacctgc
    25801 cgactgccac ccagggttcc ggcaagatcg gcaacatgct gttttctcag aaaattcctg
    25861 ccgatggtgt ccaccagcaa aatgggatga ccgcgaaggc gctccaccgc ctggtgaaac
    25921 tcctccctcg attgggccac cacgaggggg atcgccagga tgtcggcata ggtcctcagc
    25981 tggtccacgg ccccgattcg gtacgtgtcc agggaaatga cggcgggatc cagcctgcgc
    26041 ttgattttta gatgcgcggc caacttggcc agggtggtcg tcttccccac ccccgtaggc
    26101 cccacaaagg catagagctg gggttcgtcg gcgtggagga agctccccgc aaagggccga
    26161 caggtccgga tcttttccgc cagctgagcc cgaaaagcat cggcgatccg ctctttctcg
    26221 tggggatttc cctggacctg ccggagcgct ttcttcaaga gaagaaaggt ctgtttttca
    26281 tccagtccgc gggtgagcag gctatggtag agctcggtga ccggttgctc gagctgaagc
    26341 cgggcaaagg tatccttgga agagatcagc agggacagga gccccttgat ctcttcaatg
    26401 ccgttgcgca tggattcgtt ggaggcttgg gacacttcgg cctcccgctc cagagcgacc
    26461 atgacctcca ccacggactc ctcggccgac agcgccacct tccggttgcc caggatgatg
    26521 gcgtcctgtc ccagctccct tttcacctgt tccagggcct gaccgatggt tggagcgcga
    26581 aacgtcttaa cctgcatgct tgatccctac ccttcccaaa gacatggaac ccggaagcct
    26641 ctcatttctt cccccctcat gccgcctggt ccatgcggat catccccgcc gactggatct
    26701 ccagggtgtg ccccagctca ctgtgactga tcacggccac ttccggcatg aatcgatcca
    26761 ggagcttttt caaatggcga cggaccatgg gggaacaaag gatcacggga tgatgcccct
    26821 ttcccgccaa cctccgggtt tccgccccca ccccctggat gagctgatgg aggaacccgg
    26881 gatccagggc cagaaacgac ccctggtccg atttctgaac gctgcggacc agctggtcct
    26941 cgaggggttg atgcagggtg aggatgtgaa tcctgccgtt gtcgtccatg aagggcctcg
    27001 tgatggtgcg ggccagggcc tgtcgcacat attccgtcag gatgtcgggg tcccgggaaa
    27061 tcccggcatg gtcggccaaa gcttcacaaa tcgtctggag gtcccggatg ggaacctgct
    27121 cgtggatgag gttctggagg accttctgga tcgtgcccac cgtcagcggg ttgggaatca
    27181 gttcctccac gagtttgggc tgggattcgg ccaggtgatc caggagctgc tgaacggtct
    27241 ggcgatccag gagctcgtcc gcgtgcttct tgaaaagctc ggtgagatgg gtggccagca
    27301 ccgtcgagat gtcgatcacc gtgtatccgg ctcgaatggc cttttcgttc ttgttttccg
    27361 ggacccacag ggcaggcagt ccgaaagcgg gatccttggt ggggattccg tccaccgttt
    27421 cgctcacctc tcccgaactg agggcgaggg agtggcccag gaccagttca cctcgggcaa
    27481 ccacgttgcc gcgcaagaga agccgatatt cggcgggcct cagctgcagg ttgtcccgta
    27541 tgtgcagggc gggcacgatg atgccgtact cgagggccag ctgctgtcga atggccttga
    27601 tgcgggccag caggtctccg gcctgggatt cgtccaccag gggaatcagt ccgtatccca
    27661 cttccagctc gaggacgtcg ggcaaggggg gcagctttgc gacttcctcc gggcgatctc
    27721 tctcggcacc ttcctcgtcc ccgcctccgt cgaggggctg agaccccttt tgcagcagca
    27781 gcgacgcggc caccaggacc cctcccaccc cgaacagcgg caaggcggga aaccccggca
    27841 gaatccccaa aaagatcaac atgcctccag taatgcccag gggccggggc tgcatgcgaa
    27901 aaaccttgct gaaggtcgtg cccatgcccg aatccgaagc cgccttgctg acgagaatgc
    27961 cggccgaggt ggaaatgacc agggcgggaa tctgggtcac cagtccgtcc cccacggtga
    28021 ggagagtgta gttcatcatg gcccccacca ggctcatgcc gttctggaga accccgatgg
    28081 ccaacccccc caggatgttg atgacggtga tgagaatgcc ggcgatggcg tcccctcgta
    28141 cgaacttgct ggccccgtcc atggccccgt agaactcggc ctcccgggca atcatttccc
    28201 tgcggcttcg agcctccgtc tcggtgatga tgccggtgtt caggtccgca tcgatgctca
    28261 tctgctttcc gggcatcgcg tccagggtga accgtgccgc cacttccgaa atccgctccg
    28321 tgcccttggt gatgaccatg aagttcacga tgaccaggag cacaaacatc accaatccca
    28381 ccacgtagtt cccgcccacc acgaagtttc caaaggcatg aatgatcctg ccggccgcgg
    28441 tggtcccttc gtggccgtgc aggagcacca gccgggtgct ggccacggcc agggagaggc
    28501 gaaaaagggt ggtgacgaga agcagggacg ggaaaatggt gaattcgacc gcccggacca
    28561 cgtataccgt ggtcaggatg atggtgaggg ccactcccag gttggccgcc agaaagacgt
    28621 ccagcatcgg agcaggcatg ggcaacaaca tcacggacaa gatgatcacg atcccgatgc
    28681 ccatgacggc atcgctgtcg gaaatggacc ctaacctctg cagtatgggt tttgacgctt
    28741 ccgttgccac tgccatggag actcctaacc ggctttctgt ttccttctct ggtagagata
    28801 ggcaagcact ttggccacgg ccttgtagag gtttaccggg atggtttcct cgagcttaac
    28861 ctgctgatac aaagcacgtg ccaaaggcgg attctgaacc acgggcaccc cgtggtaccg
    28921 ggcgatcttg atgatctgca gggccaggtg atccaacccc ttggccacca cccggggcgc
    28981 ctccattttg gagttgtaca gcagggccac ggcaaaatgg gtgggattgg tgacgaccac
    29041 gctggcctgg ggaatggcgg acatcattcg ccgacgggcc aggttccgct ggatggaccg
    29101 aatccgaccc ttgatctgcg gatttccttc ggactgcttg ttttcctcct tggtttcctg
    29161 cttggtcatt cgcaagtccc gcaggtattg ccatttctgg tagccgaaat ccaggacgct
    29221 caggatcagc atcaccccgc acacccggac gaagaccttc caggcaaggc tgccgaaaac
    29281 cgccaggatg tctttcacgt ccaggtgggt gagggccgcg atctgctcgg tttcggacct
    29341 gagggcccca tagatgacat agccgatgat gaacaccttg atgcacgact tggccagctc
    29401 caggagggaa cgaagggaaa agaatctccc gaatccgccc agggggttga tcttggacag
    29461 gtccggcttc agaatgctga aggccaggat cacgcccttc agttgaaaca ggttgatcac
    29521 cacgcccgca aacagacagg tgacgaccac cggggctatc atcaggaaaa gggtggagac
    29581 catctgggaa aagagcgctt ggctccaggg agtccggaat cccgacgccc agaggccgtg
    29641 aagcatctgc cggtaatggt cgagaatggc cggggagctc aggaggagga cgaagccccc
    29701 caggcccatc accaccaggt gattgagctc ggtgctcttg gccaccctgc ctttgcttcg
    29761 agcctggccc agcttcttgg aactgggctt ttcggttttc tcctggctgc ttgccaccgg
    29821 ccgttcctac atcgtcaaaa atcgcgaaac cgagtcaatc ttttgtccga atccccccgg
    29881 gagcttcatt ggaagaacac ccggagcacc tgggagaacc tcagcaagga gtcccgcatg
    29941 gcgatccccc agacggaaac cgaggccccc aacaggagaa agcccaccag gatcgtgacg
    30001 ggaaaactgg ccaccaggat attgatctgg ggagcaaatt tgttgatcag cccgagtccc
    30061 atgtgggcca aaaggatgga aatggtcacc ggggcggcga tcttcatgcc caagacgaac
    30121 aattgcacgg agaagcccaa cacctgttgg acggtgtccg cccccaggac gaatcccccg
    30181 acggggaccc gggtgaagct ctccacaaga gccttgagga tcacgtgatg gccgttcgaa
    30241 ctgagaagga tcagcatggc caccacctgg aaccatccgc tcaccaacgg aacctgggtc
    30301 ccgctttgag ggtccacggc ctgggcgaat ccgaagccca tctggaagct gatcatctcc
    30361 gccgccaact ggaaggcccc gaacacgatc agcaagctca accccagaac gaccccgaag
    30421 acgaattcgc ccatcaccac ccggaccagc tcgatgggat cgaggctcaa aggcttcaat
    30481 gccgttcccg gcagaaaata gatcatggcc gagagggcca ggatcgcaaa gatcttgatg
    30541 gttctcggga cctggactcc gccgaaaaag ggaagcatca caaagacaat gctcaggcga
    30601 agaacgatgg ccagcaggac ccgaatctcg accgagggaa ttgaaaggtt cagcatggct
    30661 tcatcccgtg aagatgcgga cccgcgaggc ggagaaaatc taatgaatcc attgcggaat
    30721 cagttgaaat acctcccggg tgaagtccgt gattctcccg atcatccagg cgccgaaaag
    30781 caggagggaa aggaacgtga ccacgatttt gggaacaaag gtgatggtca tctcctggat
    30841 ctgagtcacc gcctggaaga tgctgatcat caggcccacc acaaggctca gggcaagcac
    30901 cggcaaactc acggtgagca tggtctcgag ggcttgtctg gcaattccga gcaccgcttc
    30961 ggtcgacata ggcattgcac cctaatggaa actttttacg agagacccga tcatcaggtt
    31021 ccagccgtcc acgaggacaa aaagcatgag cttgaagggc agtgagatca tgatgggggg
    31081 cagcatcatc atgcccatgg aaaggaggat gctggcaatc accatgtcca ggatgaggaa
    31141 ggggaggtaa aggagaaacc cgatctggaa cgccgtcttg agctcactga tgacgaaggc
    31201 gggaatgacc gtggtgatgg gaagggcttc ggcattcgcg ggcttttcct gctgggagag
    31261 ggagacgaag aggcccaggt cctgttttcg cgtctgcttg agcatgaagt cctggagagg
    31321 cttcagtccc cttttgagaa actcgtctcc ggagatttcc tgtcgctggt aaggaacgac
    31381 ggcttcctgg tggatcttct gccacacggg ctgcatgatg aaaaaggtga gaaacaggga
    31441 cagcccgatg atcacctggt tggggggaga cccctgggtt cccagggcgt ggcgaagaaa
    31501 ggagaaaaca acggccaacc gggtgaagga ggtcgtcatg atcaagatgg ccggggccag
    31561 ggacaccacc gtgaggagca agaccacggt gagaatcccc gacagctcgc ggggaccgcc
    31621 ggcgttgtcc aggtccaccc ggagtcccgg aaggcgaaga tcggcggccc agcaccaagc
    31681 catggactgg accgtcagaa cggccaggac tatcaggacc gctttccccc agggggagcg
    31741 acggttcccg actctgttcc tcccggcccg ccgtgccccc ggaccgctct cgggcgggct
    31801 catggatttt ccgctccgtc cgcagcggaa ccgcccgggt ttccggcagt ctcccgaacg
    31861 gatgtcagga agtggattcc ctggggagaa agtcccagga gaaagagttg ctcctgaacg
    31921 cgcaccagga gcagattgtg cttgagtccc agcgggtgct gggcctggat tttgatccaa
    31981 ggatcggcgc ccgttccccg caccttcttt ccaaaggccc gaatcccgta aaacacggcc
    32041 cccagcaccg acagcacgat ccccaagctg acggccaccc ggaccgcttg cgctcccaga
    32101 tcccacatag tctccatggc cactgcacct gcctccgttc gaccccacgg gcggacgacc
    32161 ttcctcctcc ggagggaaac cttgcctttt cccccttgat ttcgagccct acgccagttg
    32221 ctgaacccgc tcgatgggac tgacgatgtc ggtaatgcga actccgaact tctcattgat
    32281 gaccaccgcc tccccccggg cgatgagccg gttgtttacc aagatctcca agggctcccc
    32341 cgcaagcttg ttcagctcga ccacggatcc ctggcccagt tggagaagct cgttgatgat
    32401 catggtcgtt cggcccagct ccaccgaaat ctccagggga atatccagaa gaaactccat
    32461 gtctctcacc gcagccctcc tcgtggcgtc ctgatcgaga ccgggaaact gagcggcccc
    32521 cttctccggg gacaccgggg gaggattgag agggttcatt tcatcgtgtg ctttcgcggc
    32581 catggatcac tcctaccttt ttcctgggta aattccctgc tcgaacccac acccggcggc
    32641 cgttcccgag tacgcccgcc tgcggtcggc ctcaagccga cggcaacctg tggaatgtgg
    32701 ggccgggact tgatctcccg tccgtacccc gagtgcttgc ggcgggtcgt cgtccgtcgg
    32761 gccgcaacgc cgatcagaag cccgggggat tgatggtctc cgtcacccgg aacgccatat
    32821 tgcctttgga tgtaccggga aaagccttaa acttcgggat gccgcccacc cgcaccacca
    32881 ggggttcatc cacgtcctgg tccaacatca aaacgtctcc cacttccagg tgcaacaggt
    32941 cccgaccttt gatccaggtc ttgcccaact ccgcgttgac accgaccccc acttcacaca
    33001 gtcgtttctt gaagcgttcg atccagccca catccacttc cagctgatcg ctttgatagc
    33061 tggcacaaag cttggcacgg atgggttcga tggtggagta gggaagacaa aggatcatct
    33121 tgccgatcat gcgatccagc tccaactcga agtgcaccac gatcaccacg tccgagggag
    33181 gcacaatggt ggcaaactgc gggttcactt cggatcgaac gaactggaaa ctgatgttga
    33241 tgaccggctt ccacgcgtga tcgaggtcct tgaggacccc ctgtaccacc ttgtggatga
    33301 ctcgatgttc aatggaggtg tagtcgcggc cctcgatctt gaagttgctt cgacccgtac
    33361 ccccgaagaa gcagtccacg aggttgaaga tgagacggct ttccaccacc aggaggatat
    33421 gaccgcgcat gggttccatt ttgagcacgt gaaggctggt gggaaccgga agggtgcgca
    33481 caaattcccc aaacttgatc atttccgtct gagtgaccgt aatgtccacc agcctcctca
    33541 aagcgctgga caaggccacc cggtgcaccc gggcaaagcg atcgttgatg atctccaggg
    33601 tgggcattcg gccgcgtatg atgcgatcct ggcttgtcag atcgtagcgc cgtatcccgc
    33661 cggattcctc ctcctggacc tgcttggtct cgacctcccc gttggaaagg ccccgaagta
    33721 gggaatccac ttcctcctgg gtaaggatct tctccatggg ctccccgttc ctcgcttact
    33781 gaacaatgaa atccgtgaag tacacttccc gaatctgccc ttgtttcagg atccggttca
    33841 actgagagat gatctcccgc ttcagggtga tctttcctcc cgaactggct atgtcgttgt
    33901 attccttgcc ggaaagaagc atcaggatcg agtcccgcac ctcgaagccg cgggtggtga
    33961 cctccgcggg caccagcgga ttgttcactt ccagcttgag ggtcaccttc aggtagcgct
    34021 tgcctcccac atcggccaga ttcaccagga acggctcgag ttcacagata atcttttcct
    34081 gcttctgtgg tttcgccgct tccgcatcgg acggaccctt cacaaacttc acatatccga
    34141 agtagccgcc tccgcccaga agacccagaa ttacggcaag aaggatgatc ttcatgaggg
    34201 aagacccctt cttccctcct tcacccccct tttcctcccc agcgcccttt ttcgcagcca
    34261 ttgtctcgag agctcctttc cccgccttgc cacggcaatg atccgcttgt gccccattcg
    34321 acgagcccaa cgacgggacc cggttcgggc ttagccgtcc atgctcccgg accgtccagc
    34381 ccacccatcg ggctcccctc cgccaggaag caaaccatgt gccacggggg atccgggcat
    34441 ttgaagcctc cggagcgcgc ggatgcaagg tgggaaagag ggccgccctc gggggggcga
    34501 gggaagggag aggagagtca aggagttgac ccatcccgag ctgtagcgga agaaaaaagc
    34561 agggaggcgc ccggcatgga cccctccccg atattcccgg aacggcggga gcgatcccgg
    34621 gtgccccccg ggaaccgatc ccgccttctg aaaaactacc gaaccatgtt gatgctggtg
    34681 tcaatcatct gactgctggt ggtgatggtc ttggaattgg cgttatagct ggcctggtag
    34741 atgatcatgt ccaccatctg ccttgccagg tccacgttgg agccttccag ggaaaagtcg
    34801 ttgaccaggc cgaaaatctc ggggttggtg gcattgtcga aggtctggcc gatttcgggg
    34861 ccggtgatgt aaagattccc cccgtttcga accaggccgt ccttgttgga aaagctcttg
    34921 agccccaagg tgtacagggt gttgtccgtc cccgtgtcat ccacggcaac gatgtccccg
    34981 ttcccttcca cccggatact gatgtagtcc gtgggattgg cgatctggat gtctcccagg
    35041 gtcccggcag ggtcatagcc ctggacgcga tagccgtgat tgttgatcag gtagccgtcc
    35101 ctgtccagat ggaaactgcc gtcccgggtg tagaacgtct tgctgctgac gggatccaca
    35161 acggtgaaga atccttcccc gctcatggcc aggtccgacc acttggaggt atccagaagg
    35221 gttccctggg cgtagtccgt ggcaaagccc atgacctgta cgccgacccc ggcaccctga
    35281 gtgccgctgg cgaaaatgga ataatagctg ttcaccatgt ccccgaattg aatgctgttg
    35341 gatttgaatg ccgtggtgtt ggcattggcg atgttgtcgc tcaccacgga aatggccgaa
    35401 ctgtagccgt tcaacccact gagtccgttg aacattgcct gattgatccc catcttcgtc
    35461 tccttctctt ctcaaggtgt gaacttaact caaagatatg attggattct ccaaggaaag
    35521 aatcgattct ttcgcctcct ggtgaacctc cgtaacgctg ccgatggcca gcgtcacgcc
    35581 gtcctggctg tccagaacca ggtacggaac cccttccacc attcgaaagg catagacctt
    35641 tccggaaacg ctcgtggagg catccacgac attgccgttg gcgtccacgg cttccacgct
    35701 gaactggtag cttccgttcc gcatcatctc gcccgcatcg ttccggccgt cccactgtag
    35761 atcgtagcgc cccgcctgga gacttcccag gtccatggtg cgcaccgcct gtcctgcggc
    35821 atcccgaacc acgaccttca cctgctgtgc ggcagagccc agttgataag aggctttgga
    35881 gcactggccg tccgccaggt ggatcttgtt gtcggatccc acgacttcct tgccgatcat
    35941 gtccaccatc tgagcgctat tgatggaggc aagctgggtt tgctggagtt ttacgttctc
    36001 gttgagctgc gtcagtttct ccaccgtgct gaactgggcc agctgcgatg ccagttcata
    36061 ggattccagg ggattggaag gatcctggta cttgagctgc gtggtgaaca tgagcaggaa
    36121 ggaattcatg tcagagagag tgctttttgc cgactgcgtc acattccccc cggcaaaccc
    36181 tcccgtcacc tgattcgtta cgatcgacat tggaacgctc ccgtctcttt cttcgaaatt
    36241 ccgagctcca tggactaagt gacaacgctc aggagttgat cgccgcttcg aaccccgtac
    36301 acatgggtct ttgaagcgat ttcctttccg tatcctttgg cggttttccc ccccaattcc
    36361 gcaaccttct tagcggaccg gccttgacgg taaagggtcc cgtggccctt ttgatccttc
    36421 actcccacaa agaattcccc cagcaacagc ccttgttcct gcatgcattg ccgaagctcc
    36481 gaggtgtgct gggagagaag gagccgggcc tgttccgttt ccgtggacac ccaggcggtc
    36541 acgtgattcc ggtccgtctc gacggtcaag gtgaggcggc ccaggtcttt gggctcgagc
    36601 tcgagggtca ggtggtttcc tttccgagtg gtcaattctt tgaactcatg ggccaagcct
    36661 cgaggccagg aagcgtcgcg caaggacagg tgcccggttt catccgagga tgcgggagcc
    36721 gcggcctccg gagtcccccc ccgcagctgc tgaacgaacc cgtctcgaga aatgcccggc
    36781 ccctcccacg gctcggagct ttgagtggga gagaccgatg gagagggctt ggtttcatga
    36841 gccgaaaaac gagccgacgt gtcctcggaa gggaatctcc cgttggtttc gggacttgag
    36901 ccctcacgcc ccgttgtttc ctgggtatcg gccgagtccg agttgaaagc cgaggcagcc
    36961 ggctcgagag gtcgggggga cctggccgtg aacctccgcc agatcgactt ccactggaac
    37021 ccttcggatt ggtccccatt cgacggttct tccccgggaa cggccttctc gaggaccgga
    37081 agccggttcg gttcgccctc gaggacctgt tcgttcgccc ctgcatccgt tgcttccgga
    37141 accggcactt tttccatggt caacttgtat cgaggccgct ccgactcgga acgaatttcc
    37201 cgggcctccg aaagcggcat tctcgtcccg cgcatctcgg accgggatcg ctccgaaccc
    37261 gccgggaccg cttccgaaag aggaccgatc ggcccgggtc ttccctgaac ccgccgggag
    37321 ctctcgccaa ttgcctcggc catggagacc atcctttccg tcacacgaag ctcctcgccc
    37381 tccctctcac ttcccaccag caagtcggac agcctcgagc ctccgctctg cgcccctgtc
    37441 ccttcccgag gccgttggga ttcgggaacc gtcctctcca cctcgaaaag atcgatgtcc
    37501 ctcgtccttt tatccgtgat gccgggggaa ggagagtcct tgatcgaacg accgaatttc
    37561 cccgcggcat ccccacccgc cccgtcaaac tgatgggaaa acccttgccc gggttctggg
    37621 gcgggctgga cccgggagtc gcttccctcg gttgtggaag gagacttatg ggaagaaaac
    37681 ggccggtccc ccgctgtttc caccgtttgc accgaaggcg acgagggagg gggtccctga
    37741 tgccccggcg tgctttccgc gtcctttccc gaaaggtcca tggcggcaag cgaagcaaag
    37801 gtttccggca cgccgggaag aaccgacctt cgggagactg cttcctgacc cgacaacccg
    37861 cgcaatgatc cctggggatg agaaccccgg tcctcactcc ctctccggtc ccccagccat
    37921 ccccggaagg actcctcttc ccccgaaatc ggaagtccgg gaaattggtc cgaatccttc
    37981 gggactcggg aaacctggtc ggcctccttc ctctcagggg tttcgaccga cacggccgac
    38041 tcggaccccc ggtcgccgtc cttcaagtca gaggctggga ctgccggtcc gtcgaccgtc
    38101 tttgcccgaa gccccctcaa aggctcttga ttcaggcaaa aggacggcag tcgggtgtcg
    38161 gacattcttt ccacgtgatc ggcccggacc aggtcggaca ccgatccgat tgcggaggag
    38221 ccttccgtca atccggttgc cgggggcaca ctttgcaggg aaagggcctc cgttttggac
    38281 ctctcccggt gcacctgccg aaccacccgg tccaggagct ccacgaactc ctgaaggttg
    38341 taggattcct tcatcaaggg ctggatctta cacgacaccg gggtgcccgt cggggatttg
    38401 tactggaggg agtccaacag cagtcgcaca ttccgagccg acacttccgg ctctttccca
    38461 tcgaggcgct cccggggagt cttctgcgcc tggaggatcg acaggaggtc cttataggaa
    38521 agcatcccct gggaatcggc tgcatccttg caggcctttc ttgcctccgc aggcaactgg
    38581 agatgcgcca tgatcttttc cgaagccgtg ggattcgtga aatagagtgc ctctttcacc
    38641 cgctcccggg aaattcttgt gggcttgcgg ggaagggccc gctccagggc ctctgtttcg
    38701 ggggcggcct tttcaggcgg ctcgaccggg gtttccggac ccgcatggga aaggacgaca
    38761 acccctccgt tgcagctccc gcctgcaacg ggtttcgcgg tccctgcggg ctgaaccggt
    38821 tccctttggg aatcacccgt accggccgcg aacatctcca aggccgcatc gtttccccgc
    38881 atggacgaag gattcgagcc caggacgaac atcttctcct ggttttgggc atccaactcc
    38941 ctggaaaagg aaaactgggc gtccaggatc ttgctcccca attttccgtg ctcgttggac
    39001 aagaccccca tgagcagtcg gcccgcattg agatgattgg tttgcatacc gcatcctcgt
    39061 ctgaggggtt tccgaatcga cctgtgggtc gacccggtac aaagcaaccc ggatgccggc
    39121 ccttgcgtgc aattgtttat gttgtgttat cggctatttg taaagagtgt gaatgaggag
    39181 gtcgccacga atcggggagg caaggcggaa atttctgcag gatcgccgag cattttctgc
    39241 cgccgggcgg ggagttcttc cgggcagagg ttctcatgac gggtgaaaag tccggccccg
    39301 gaaggaatgc aacctcccgg aggccggtgg tctccggatt gcgaggattt gcaggaaacg
    39361 tcccccttgt gtggaagcgg tccgcgcgaa agatcgaggg cgcctgcctg gatccgccca
    39421 cctgaagaat cggaggtcgg gggggaaacc tgtctcgaga aattccctca tacccacaaa
    39481 cggggaatgg atccttttac ggtcttgtgc tcgaagggtt cgaattcacc ccgggcattg
    39541 gcgatgaacg tcttgatggt ggccgtgtag cggaaaaagt actttcccga ggaatctttt
    39601 tccgtccata cgcgggtggc ggtcttgagc ttgatcttcc actcccctcc ctctccggcc
    39661 cttttcatgc tggtccggga cgcggtgagc cccgtcatgg acaggggatc gtcgtggaag
    39721 atgcggaaat tctcctggga cacctggtcc acccacaggt cccggagggg ccccccgtag
    39781 caataccggc cgctgtcggt caccttcctc accaggacgc ccttcttctt ccccctggtg
    39841 atgggtttga ccttgcaaag gtacctgctt ttccggctct cgacggtagg aacatattcc
    39901 gaggaatcca cgcacaggat ctgaggatcg gaaggaagga gcttcttgag acgccggtcc
    39961 tgggctcggg gtctgcgccc gggcaaagag agggcgcttg ccggggaatc cggactcctc
    40021 cccgtcacca ccatgaggat cgggttttcc gtgctttgcc acagggttgg aaaaagggaa
    40081 ggggaaagag acaaccgaac ccgccaaccc cgcttgaagg tgtgcccaaa gacgttcagg
    40141 ggaaaactca cgtgaaaagg gaccccggga gagaccggtc ggggggctgc catgtctccg
    40201 tcccggtagc acaagttgaa gaaagagtaa gtcaccaagt gggaagcccc cgtataggga
    40261 ctgatttcac agaggcgaac ggcaacggaa gccaatggtt tgttgcagac aaggttcagg
    40321 ctcaccgtcg ggtatccgaa acagtccatg tcttcggcaa ggggtcccga gtcaaaatgc
    40381 agagactggt tgtcggcttc ggtctgatct ccaggcagat cgtcgttgcc gcattgcccc
    40441 caggacgaag tctccagcat tttcgtgccc aggaccaggg gaccagggca gattaggtac
    40501 cccgggtgcc ccggctcctt gggggacagg gactggtttg gccacaggta gaagaccttt
    40561 tcccggaccc gggtgggcca ttgggcatct tcggaggccc atttcccccg gtcgggggtg
    40621 cagcatgacc tgctgggctc ccgggattca cccaaccata cggtcagctc cggcaactcc
    40681 tcccggggat ttgggtccgc gtccttgagc caccggtccc accagtggtg gagaaggtaa
    40741 gggagccagt tgatccgggg cccgggatcc cccgcgtggg gcttcgcgtg tccccaggga
    40801 cccaacagac cctggacggc catcccggcg gaatgcagcc cctcgaccgc acggaccacg
    40861 gggttcttgt agccgtccag gtatcccgag acaatataga cgggaacctt cacgcggtca
    40921 tagcgatccc gcacactcga agagctccag tatgaatccc gggtttggtg ggagccccag
    40981 tgcttgaacc agaaatccgc attccgaatg cgctcccgcc acatctgttt ccaacggttt
    41041 cccacgattt ccgggtcggg gggctgtgcg atccagcccc acatggagga aggccatccc
    41101 acattgtcct gcatcatggc tccccccatg tagtgaacgt cgtcgttata tcgatcgtcg
    41161 cttccgcaga tgaccagcac cgccttgagc tcggaaggac accggtcgtg ggcggccacc
    41221 atgagggagt tgatggcgct ccaggacttg cccatcatcc cgaccttgcc gttacagagg
    41281 gggtgccgcg caacctgctc gatgacctga atgcagtaga ggacttcctc ctccgtgtac
    41341 tcgtcggtga ggattcctcc cgaatctccc gatccctgga gatccactcg aaagcacagg
    41401 tactcgtgat aggcgaagta cgggtagaag gcgctgtccc ccagcatcat tccgtcccgg
    41461 cgccgataag gattcaattc gaccagggcg ggaaacttcc tgccttcttt ggcggcatcc
    41521 gcgttgtacc aataatggcc ctccaggcga acggggtctt tgcccggata ccggacccaa
    41581 aaggaaaggt tttcgggatc ggggaccttg tcccgggttg gaaagcactt ggaatcgtta
    41641 cagcacgccc cttcggcaga aggctccgaa cagggggccg ggggctttgt ggtttgcccc
    41701 atggcttcga gggccaaacg gggagcaaaa gcagccccaa cggcccaagc ggcgcccatg
    41761 agaaaacttc ggcggtccgg agaaagcggg tcgggcaatt ccggcacggg ggcctctctt
    41821 tctgcgccat cacgagacga gtccttcaaa gaccgagaca taaccccctc ctctccagaa
    41881 cgatgaacgg ttcggttcac gcggacccaa cttcaaggtc cttccgggac gttgcccctt
    41941 gccggtatcg aggtcgcgtc ggcatttttg cacaagagat ccgggatggc aagaaacgag
    42001 aaggagacgg tttcaagagg gcttcgaccc ggggccacgc agcattccaa gtccctgggg
    42061 cgatcgcctc cgctccctgg ggcccctgac ggcaggaatc cccgaacggt tgtccacggg
    42121 gtttcggcgg ggtgccgatt gcccgggata cccggttcgt cccggagagg agacgacccc
    42181 ttaacggtga gacagggatt cggagatctt ggccgccttt ttcacgtcca tgtgggagag
    42241 aatgttggcc gcctcatccg aattcatggt ggagatgatg tggactacaa ggttttcttc
    42301 gaggttttcg agcaacttgg cagcttcctt ggccttcatg ctgccgtaga ttttggctag
    42361 ggaccggatc ttgttgtttt gagtttcctc tttttcggtc cggtaggcct ggatttcttt
    42421 ctggatggga atgagctcct tgaacttccg ttcgacctcc tgttccattt gagacaggta
    42481 ttcttccttc tgtcggagct tttcctctct ctgtttcagc tccgcttcct tacgttccag
    42541 gtaggaagca aggtccgtta cgggttgggc gccggggccg gaggaagcga ctttggtcgg
    42601 tgccggcttg gcgggatctg ccttgacggg gggggtggaa ggcacgggtt ttgccttgct
    42661 gtcctccgcc aggacctcgg cgggaaaggg aggcgcctcc gctccgtcct tctgaagaag
    42721 aagggaaccc accgttacgg ccagtttcag cgagagcagg aaaaacccca ggaacaccgc
    42781 accgatgaga gatttaggat acttactctt cttgtaactc atcttcttcc gatctcgtcg
    42841 tgatcttgtc tggagattcc tgataggacg cgatttcgtc catgcgcttc cggtcttcct
    42901 ttcccagcag atatcgatac tcttcccggt ctttctcctc caacaattcc agcattcgga
    42961 cgtcttttcg cttttccagc agttccttct ttcggttttc caccgcgcgc agagcgttct
    43021 gccggtcatg cctgagaaga agcaaatgtc gttccaacga ctggaggaat tccttatgac
    43081 tgaggtaatc ggaaacggtc atgccttcgg attgtcttgc ctcgagggcc tggacctgcc
    43141 cggcgatttc ggcgctgacg gcccgaatcc gggattcgag ggcgtcgtat cgggcttggg
    43201 ccgcagccat ggccacctgg gcctgcttga gaagatgttg ccggtgtcgc agaacaatgg
    43261 caaaacggaa ctgaaaaggc atggcactct cggcttccct gacccggccg ggtctcgacc
    43321 cggggtgaac cgggaaagtc ggaattcatc ccgtggaaac gaggatgcga acggttttcg
    43381 tttcatcctc ccgggggact ctcctgcccc gtcctctcct attcacgccc taacccaagg
    43441 gtccggagct gctcccgggc ctgttcgatg gaacagtatt cttccacagg ctgggtgagg
    43501 tacccgttga gccggtcaat catctcgatg gcgtagtccg tgtccgggtg gctccccttc
    43561 acataggccc cgatccggat catgtcttcg ctttgcttga aatgggacag ggtctgaatg
    43621 aaacgggaac tgaggacctt gtgttcctcg tcctgcaacc ggttcgtcaa acggctgaca
    43681 ctcttcaaca cgtcgatggc aggataatgg cgggtctggg caagcctacg gtccaggacg
    43741 atatggccgt ccaaaatgga acgtagggta tcggctatgg ggtcctccat gtcgtcccct
    43801 tcgaccagga ccgtgtaaat gccggtaatg gatgcttcct tgaagttgcc cgccctttcg
    43861 agaagacggg gcaagcggct gaaaacactg ggcgtgtagc ccttggtggt agggggttcc
    43921 cccgcagcca gcccgatctc ccgggcggcc atggcaaatc gcgtggccga atccatcatg
    43981 aggagcacat ccattccctg gtcacgaaag tattcggcca cggcacacgc catgaacgcg
    44041 cccctcaacc ggagggtagc gggctgatcc gatgtggcca cgatcaccac ggaacgtttg
    44101 accccctcgg gccccagttc atgctgcaga aattcattca cttctcttcc ccgctcgcca
    44161 atgagggcga tcacgttgac ggaagccgaa gtataccggg ccatcatgcc cagcagggtg
    44221 ctcttgccca ctcccgatcc cgcgaaaatg ccgatgcgtt gcccttttcc tacggagagg
    44281 agaccgttga tgcaccggac tcccaggtcc agctgttcgg tgatgatggg gcgatccaga
    44341 ggattggcga cattgcccga gagcgggtaa gggatgccgg gaggcaaggg gcccagcccg
    44401 tccatgggct gccccaatcc gtccaggaca cgccccagga gagaagggct cacattgacg
    44461 gaacccgcca gctggtacgg ctggaccaga caacccgggt agatcccccg caagcgcgtg
    44521 agggggctga gcttcaatct cttctgatga aacccgacca cctcggcaaa gatgggattc
    44581 gccccgcggg agggggtgat tcgacagata tcgcccaaag aacagtccag cccgtcggct
    44641 tcgatcatgt tccccaccat gtggaccacc ttcccgtatt tttcgtagcg ggggcaatcc
    44701 ttgagggaag tcgggtcttt gaatcccaga tagttaggat tcggaaggat catcttgatc
    44761 tgcccgttgg gtctgagctt catcggcccc cgtgagggcc ttctccagga gggccagtcg
    44821 ggtatccagg gtgctgtcca acaactgcag gggagtctcg atcctgcagt ccccccgttc
    44881 caggccggga tccggcttac atatccggga acgagcgggg gagaatccgg cttccccgtc
    44941 ttccagggcc ttttccacca atcccacgtc cttgggatgc aaaaagatcg tcacgggaga
    45001 atcttccccc agctcgtgga gcaagctttc cagagtttgc agcaggattt ccgggtgggc
    45061 gtccagttcc tttcgaacca tgtgacgcac ggcggcaagg accgtttgca cgaaccattc
    45121 ccgatagtcc tcgatgatgc gggagggcaa ggcttccaag cgggagacga ccctttggag
    45181 gtgctcttga gcgatgagca tgctctttcg cccaaactcc atcccgtcct tcaccccctg
    45241 ttcgtacccc ttgtcgtagc cttccttttc gatctccagg gcccttcgct ccgcgtccat
    45301 gaggcgcttt tgaatcaggg attccacatc ctccggggga gccggcgcct tggaggaagg
    45361 gcttgcggca ggaacggagg agacccccga gacggccgcc acatcccctt cgatggaccg
    45421 aaaatcaaag gggatgtggc ggccgtctcg ggggtctcct ccgttcctgc cgcaag
//
LOCUS       DAOOVC010000017        42578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_576_length_42578_cov_6.285963, whole genome shotgun sequence.
ACCESSION   DAOOVC010000017 DAOOVC010000000
VERSION     DAOOVC010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 42578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42578
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_576_length_42578_cov_6.285963"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..5962
                     /gene="uvrA"
                     /locus_tag="PLM79_04075"
     CDS             305..5962
                     /gene="uvrA"
                     /locus_tag="PLM79_04075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOV85515.1"
                     /translation="MSREKFVRLRGVRQHNLKNLDLDIPLNRLIAVTGVSGSGKSSLA
                     LHTLYAEGQRRYVETFSPYARQFLERMDPPDADLIEGIPPGIAIESGTAVRSSRSTVG
                     TITEINDFLKMLFARLAVPHCPNCGRPIRQDTPQTIYRSLEALPEGSRLLLCFPYVAE
                     KSAVWPRHLISKGFLRLYTSGRTVDLESMDKKAVGRGSGKEHLVVVDRIPWGKVPEER
                     IMDSITTAFTEGSGRMAAVVLPEELLWFSSRLSCADCKPEVSVPPPSPNLFSFNSPLG
                     ACPECRGFGRTIGVDWDLVVPDSTLSLDQGAVKPWGTDREEYRELLEFCRKEGIPTDV
                     PFGALSEADRGKILHGTPDYYGVRGFFEWLETKTYKMHVRVFLSRYRSYERCKTCMGT
                     RYQRDTLLYRLKGATIGEFCSWSIERCRQFLKESWPEPTADAAADLLVQEIRSRLDFL
                     HAVGLGYLSLDRQSRTLSGGEVQRVHLTRALGSALVNVLYVLDEPSVGLHARDQGRLM
                     TQLRRLVDMGNTVVVVEHDPEMVRFCDEVIDLGPAGGEKGGRVLFQGPPEDLPRCSES
                     LTGRYLGGPGAARMPRARRTPDGRPALTIRGAREHNLRDVTCTIPLGLLVGVSGVSGS
                     GKSTLVEKTLYHNWLRRTGRSTETPGICDGLEGAESVREMILVDQQPIGRTPRANLLT
                     YTGALDPLRKLLAQTPEAVARGLSTRYFSFNVPGGRCESCRGEGFERIEMQFLADVTV
                     RCPQCNGRRFKDDILEIRVRGLSIGDILECTAQEVLDRFADVEPLVRALQPVVAIGLE
                     YLRLGQPLSSLSGGEAQRLKLIRYLVSNGPSSLSKNAHKLFLLDEPTTGLHPHDLQKL
                     LRVLDRLVNDGNTVVVVEHNLDLLDACDWILELGPEGGEEGGRIVAVGSPEDIAQNPD
                     SPTGQFLKRRHEGAIPAPAGEGGPFIPSGSAQRSPPAVPSAEDAIMVRGAREHNLKLS
                     RLDLPREKMTVLTGLSGSGKSTLAFDVLFAEGRRRYLECLSTYVRQYFKVLEKPDVDQ
                     VLGLPPTVAIEQRTSRLNRRSTVATITEVYHFLRLLYSKLGRQRCPDCGRELTALSHD
                     RILALVEREMVRGEAKVLAPLVRGRKGIYRDLFLRLRKMGFQTVRVDGQWKDLALVPS
                     LERHREHDIEVLIPHRTSASSPAAELSSAVRKALALGGGTLHLAGAETRVLSDRLYCP
                     TCNRGLAPLDPRLFSFNSRLGACPSCSGLGIVEKINEERLLGPPHLGLREGLWDFLQT
                     SVFRGRYRSWGARLHQYWTQELQLNLDRPVGELEPDLRHAILYGRDKGSPGLLAVLEK
                     GPSDEKIQKALAPFREEAICPECGGARINRQARSVDFKGRTITDLVDLSIRDFQDLWK
                     SVDLGETERPVGLPILREILTRISFLREVGLDYLTLNRSGDTLSGGEAQRIRLAAQLG
                     SNLLGVCYILDEPTIGLHFTDNDRLLNSLEKLKDKGNTIIVVEHDPETMRRADVLIEL
                     GPAAGNEGGRLTARGNFQSLCRDEETLTGRWYGTSVDHLYGLTRRRPFAPTGWIEVLG
                     AAERNLKGIDVRIPRGALTCVTGVSGAGKSTLVHEVIYRVLSRLLGRDNGQGANIRGE
                     LMGIEGIQRVLEVDHNPIGRTSRSIPATYVGVWDEIRRLYAMLPDSRVRGFTPGHFSF
                     NVKGGRCENCKGQGQLKVEMHFLPDVFVPCESCGGRRFNEETLAVRLKGKNIAEVLAM
                     TIEEASRFFLAFPGISRSLRILNELGLGYLTLGRPSPTLSGGEAQRIKLAGELGNHRG
                     PTLYVLDEPTTGLHRADVKKLLEVMWALTDRGHTVVVIEHNLDFVWASDYVIDLGPGS
                     GDRGGRLVVAGTPGEILNHGAVSLTARALEKHLEAEARGCRAG"
     gene            complement(6099..7610)
                     /locus_tag="PLM79_04080"
     CDS             complement(6099..7610)
                     /locus_tag="PLM79_04080"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOV85516.1"
                     /translation="MSKRILIFDTTLRDGEQVPGAKLNKRQKLEIAQQLAKLGVDIIE
                     AGFPCSSPEDLQAVKAVSEQVKGPVIAALARAVQQDIDIAWEGVKDAERPRIHVFLGS
                     SDIHLQKKLRRDRESAIEMAVNAVRYAKRYCEDIEYSTEDASRTDFDYLCRVVESAIG
                     AGATTINIPDTVGYAVPEQFGDLIRRLRERVPALDNVVLSVHCHNDLGLAVANSITAV
                     RCGAGQVECTVNGIGERAGNASLEEIVMILRTRPRTFDAVTGIRTQEIYPTSRLVSRL
                     MNIPVQPNKAIVGSNAFAHSSGVHQDGILKDRATYEIIRPEDVGIRQHKIVLTARSGR
                     AALRHRLNELGFSVSPEQFERVYQRFLSVADRKKEIRSADLRSIVEIELTRVAETFSF
                     HSLQIMSGNTMVPLASVTLVKDGEKITDAATGNGPVDAAFNCIERIVGRQGVLRDYDL
                     KAITMGKDALGEAMVRVEIAGSVYSGIGTSSDIIEASARAYLNAFNRFFVNSH"
     gene            complement(7949..8080)
                     /locus_tag="PLM79_04085"
     CDS             complement(7949..8080)
                     /locus_tag="PLM79_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85517.1"
                     /translation="MEALQVMEEANRIIEDFPARKYAFYITVFAVEIALCFAIGLMI"
     gene            8304..9752
                     /locus_tag="PLM79_04090"
     CDS             8304..9752
                     /locus_tag="PLM79_04090"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85518.1"
                     /translation="MNHGKSTSRSAKSPQGEKNTGGGKRAGLFETFDVRTQAEPSSLG
                     PVPADVLDLMERLRAKGHGVWVVGGAVRDLLTGVAPRDWDVATDAETREILDLFPKVI
                     PLGLRYGTLQVHTSQRQVEMTSLATAADGNIFKDLARRDFTINAMALVFPTGELLDPH
                     KGKADLGKKILRAVGNPTARLMEDPLRMLRAARFVSTLGLRPAPSIFAACRDQVYGLD
                     RTARERIREEFFRILVGTRVVQAFEFLRRTGILEKLLPEFRLDGSFRGGKRLFLRNYR
                     HAVGTVSLCPLDGRVRLAAFFHCLGNPCPAVNGDGLGEWIEERDVTLSAASRVMTRWK
                     TAHKDMREVLRLLEHWIPPGGEDLSPPALRRLVLGVGEELLEDLVHFGYADRRDAGNE
                     RCIRAFDQLVLRIKEQMDGGWIVGRSQLDLSGEDIMETLGIGPGPRVGEFLKRLHDFV
                     IEDPRANHREILLEKLRQISPKTGPPAEGCPG"
     gene            10221..10913
                     /locus_tag="PLM79_04095"
     CDS             10221..10913
                     /locus_tag="PLM79_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOV85519.1"
                     /translation="MLVKNWMSKNVITVGEEDSMQQAMSLLKEHKIRMLPVVREGKIV
                     GIVSETDLKRASASDATLLDVHEMLYLISKIKVKDIMTRQVVTVHEDLTVEETAEVLR
                     EKRISGAPVVDHGGQLVGMITKDDLFNVLIALSGLGKKGIQLAFRVEDRPGSIKDLTD
                     IIRQHGSRLASIMTSYEGVPKGFRNVYVRIHDVDREELAVLERELREKATLIYVVDHR
                     ENKRRIYHPELE"
     gene            complement(11024..11728)
                     /locus_tag="PLM79_04100"
     CDS             complement(11024..11728)
                     /locus_tag="PLM79_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85520.1"
                     /translation="MLRVENVDAGYGDIQVLWDVSFEVKGNEFVVLVGANGAGKSTIM
                     KTISSLIRPTRGQILFEDRRLDVLPPYKIIEAGIAHVPEARQLFPEMSVKENLELGSL
                     TPAAKARRSETLDWVFTLFPRLRERHKQMAGTLSGGEQQMCAIARGLMSRPKMILFDE
                     PSLGLSPLLTQEIFQLARRIHSEGLTILMVEQNVKQTLAICDRAYVLENGRIVLEGKG
                     KDLLEDEKVKQAFLGI"
     gene            complement(11733..12446)
                     /locus_tag="PLM79_04105"
     CDS             complement(11733..12446)
                     /locus_tag="PLM79_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85521.1"
                     /translation="MAYFQTEHLTKNFGGLIAVNDLSFQVEQGQIFGLIGPNGSGKTT
                     VFNLITGYYPQTSGTMRFKGSTIDGLPTWKVCKLGIGRTFQVVKPLRRMTVLENVMTS
                     AFNRRQSMQAAREKALEVLEFCELSKKLDYPAKGLTIGDRKRLEIARALATEPELILL
                     DETMAGLTPQEQREGVELIRRVRDTGKTIIIVEHIMHVIMNLCDRILCINYGQEIALG
                     TPAEVANNQAVIEAYLGKE"
     gene            complement(12467..13429)
                     /locus_tag="PLM79_04110"
     CDS             complement(12467..13429)
                     /locus_tag="PLM79_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85522.1"
                     /translation="MRINRWVLLGIVLFFLAFPVLVPSDYYRHIFIIALMWVLIGSSW
                     NLLAGFTGQVSFGHAIFFGTGAYTAGLCVTKLGISAWWGILLGGPVSTLVGLFIGWVC
                     FRLRGPYFALATIAVGEIFRLVAIDWRSFTEGMEGILIIQTFRSKLPYYYLGVALAAL
                     CMFAIHRVVYSKWGYYFVAIREDQDAAESMGISAFRYKSLSLMISSFFTGLAGAFYMN
                     YMAFIDPHVVFNLHDISIMAILVGIIGGVGTIWGPPVGAFIMVFLQETFRSSFFGLFP
                     RWVSESHVLAFGLLVIFVIRYLANGVIGDWPKIRRLWTREKAYS"
     gene            complement(13443..14318)
                     /locus_tag="PLM79_04115"
     CDS             complement(13443..14318)
                     /locus_tag="PLM79_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85523.1"
                     /translation="MEILVQTLVSGVLIGGIYALIGIGMTLIMGVMKIINLAHGQLMM
                     VAMYITFVLFEYFGLDPYFSLLVSMPALFLLGIFLQKYLLNPLLKVDSILPENQVLMT
                     VGIGMVLTEIIRFIFRSDYRSVKTAYSATALYIGPISLNFPMVVAFGIALLLTAALFF
                     FLLKTDLGKSVRATAQNADAAMIMGINAHWITVLTYGLGSGLVAAAGTLLLPIYYLFP
                     DIGGPFTLKAFVITMLGGMGSTIGAILGGVVLGVAESLGATYISMALKDAVGLVIFLL
                     VLIFLPGGLKTLTKV"
     gene            complement(14390..15583)
                     /locus_tag="PLM79_04120"
     CDS             complement(14390..15583)
                     /locus_tag="PLM79_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV85524.1"
                     /translation="MKRIATVCLALCMAAALSAGPTLAQDSIKVGIILPLTGGQAAFG
                     EIEKNSFELALEEINAGGGVNGKRLDFIFEDDTGKPEVARSAAEKLITKDKVVMLGGG
                     YGSSETFAIAGVAQQNEMPFLVNTGSADKITEQNWNYVFRLNPAVSEYPSALESFLTE
                     VVKPKTAAILFEDSNFGSGGSKTFQETCNRLGIKVLLNEGYSKGGVDFKPLLVKVKQA
                     NPDLVYMISYIMDASLLMSQSRELRLSPKVFVGGAAGFTLPEFPKNAGKASEMVYSAT
                     LWYQTLPYPGAMAYYDKYVKKFNKDTEYHGAEAYAAAFVIADALKRTKSLTPKDVRDS
                     LAATDMMTVFGPVKFISYDKKTNQNKLPTYLVQWIDGKLELVWPRNVATKPFVFPIDW
                     EKTWK"
     gene            15787..16779
                     /locus_tag="PLM79_04125"
     CDS             15787..16779
                     /locus_tag="PLM79_04125"
                     /inference="COORDINATES: protein motif:HMM:NF021832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HOV85525.1"
                     /translation="MRYAMGTTPDHEIVRSIEPVVLQSEHVLFHPEAVKDAADRLEEQ
                     EDFPGLSWKHPCHFFDGGEETARWILVLDVLNHCFWPSPGQKTWTVIHGGRRWSGYWG
                     LAASLNRAMERRTPVTRASWLASLEEEDLRRVFAGEGDIPLFPERLRNLREAGRILEE
                     RWGGDFVNLIEEASKSALKLVRLVVESFPGFRDQALYRGYKVNFLKRAQLLVWDLYCA
                     FEGKSFGEFHDLEALTAFADYKLPQVLRELGVLSYDSPLAARVDRREELQPGSHEEVE
                     IRALTLWAVEAIKEELLQRGRNLMSAQIDRGLWNLGQRDAYRRRPYHRCRTTYY"
     gene            16993..19653
                     /locus_tag="PLM79_04130"
     CDS             16993..19653
                     /locus_tag="PLM79_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HOV85526.1"
                     /translation="MEYRYRRESFGELPVRPEHLDIYLDFRPDFVEAVNCMRMTPRGP
                     MEVLELDANGLDILAVEWRETSPDSPGSWEALAYEDRRDRNKLVLFFPRPVSRDRPLW
                     VRTRTRCYPSDHILEGIYKDVTPPGAPQQYMSQCQQWGFQRIMPILDDCRAKCTMTTT
                     LEGDARYTHLISNGNVNLESNPQGVPVPKPGDPRRRTITFENPVPMAPYLFIACAGTW
                     DTLTDRVTYPDGRTVRLEYLVPPGMSPYARVPMEILKKAVLWIRETQDYTYTGDTYRT
                     ICMTKSNFGGMENVGNTTIVTDAALVHEHSLDASLLYAHAVIVHEFEHNQCGSETTMD
                     TPFDVWLNEAYTVDVERRFMADRFDPTLVRLLQVDGIRNPLLGPLAIEDGGHVGRVVR
                     EGFNDPDELIDGVTYVKAAEIIGMLRRVLGEENFLAGKTLYFQRYRHGNADTDQFFAC
                     FEEVSGTSLEPFKKGWLYRIGYPRVTAETSFDPAKKEFHIRWRQEVPPGEAPFHIPIE
                     LALVGEDGKDLPGTGQVFQLNREEASLTLSGIEAEPAFASMNRDSSFYGTFSHRNSTP
                     DLLRKQVLLDPNGHNRVDAMRKLTDLQRIRLLEDPRGEIDPRWLDLFEDLLADSTVAP
                     GLQSYLLRIEEQPVDRDYATWYPELAAARETMMRALNGRYRAKLLDRFEGLRAAPPPP
                     ESEPWTGLEERMLKNVLLDLIVVDDSPQSHELILDYFRNAANPTDRVAALGALNRSGS
                     PQRRPVLEEVYEAWHGHLSGYANYLRVVAGGTRPDVFDLIDREKRRSTFDITQPTWSR
                     ALFVSMAANNKMLWTEKGIRWMTAAIIELAPINQTTTSRMLNAFQHVRRLRPPLRERV
                     LEALEEIVSRVGREASPTVYGQASSYLAGR"
     gene            21039..23273
                     /locus_tag="PLM79_04135"
     CDS             21039..23273
                     /locus_tag="PLM79_04135"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOV85527.1"
                     /translation="MEEVSDFEKYVAGKSPEKLSFDIKQFGYDLFLAPPTLTGPVQRT
                     VPVGPEYVMGPGDELRVNVWGSIEGVWNVVVDRDGAITLPKVGTLGIAGLSFQETKAV
                     IQKELAKQYTDFEMNVSMGQLRGMGVYVVGKARKPGAFVVSSLSTLLNALQESGGPSK
                     VGTMRDIQLKRNGETIAHLDLYDFLLTGNKENDVRLMPGDVIFIPAIGPMVGIAGDVK
                     VPAIYELKGAARVSDVIKLAGGTTAKAFTQRVQVERFSKRTNKAILDLNLGQLKGKND
                     ILLEDGDVVKVFAVTENVTNRVVLKGNVRRPGEYEWRKGMRVRDLLKSTEELLPETLM
                     ERAQIDRLVPPDYHLEYETFDLGKVLLEGSESENLLLAPYDTVIVFNKWEVKERDKVR
                     SSGALNKPGEFDYRKNMRLSDLIGLSGGLKYYASAKEAELTRVKPTPEGPVTEKITVY
                     PSSALQGNPEADILLQENDYLFVRAIPEWDLYKLVTVGGQVMYPGAYSVDKREKLSSL
                     LTRAGGFTDVAYLQGAVLVRESVKRFQQEQINRMVDKLQRELLSSGSVNIGTASTPEE
                     AKILQEQEKQKALFLESLRHLQAQGRLFITLDRLDKLRGSQNDIELEEGDSLYIPTKP
                     ATIQVIGAVYNNPSAMLFEQGRDVSYYIRMSGGFSENADSKNTYVLKVNGRALKPKGK
                     FLAWDRDSNSWRSSGYADLQPGDTIVVPERLEKVAWMRNIKDIAQILFQVGTAAGVVV
                     AAGL"
     gene            23789..25051
                     /locus_tag="PLM79_04140"
     CDS             23789..25051
                     /locus_tag="PLM79_04140"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HOV85528.1"
                     /translation="MKEVLQDQVDEIEEVHLLDYVLVLLKRKKLIAAITCAISLLSII
                     YVLVIPPIYEGTARILPPSKGGASMGLLSQLSSIAGMASALPGMPGTPSDLYVGMLTD
                     SRTIADAMIDRFKLMEVYDKDNRVDTRNALSKRLRAMSDRKSGIITITVEDEDPKRAA
                     DMANAFVEELAKINNTLAVADARQKRLFFEGQLKETITALRVAEEDLKSFQEKTGAIQ
                     ISSQAGAVVEGIASLRAQIAAKEIQIKVMRTYFTENSRDVRRAEEELAGMKEQLKKLE
                     AGGDKSYGSTIIPTGEVPALSVEYLRKLREFKFQETLYEMLSKMYESARLDEASDVSS
                     IQVVDSAVVPEKRSKPKRTLTVVLATVLGFFFAVLASFFAEYWEKMSQSPEDCQRLEK
                     IREHLDSIRNNRALTWICRVFKRGSGGS"
     gene            25181..25633
                     /locus_tag="PLM79_04145"
     CDS             25181..25633
                     /locus_tag="PLM79_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV85529.1"
                     /translation="MKTEEYKKIIQMAIGNEVAAYEFYKSVCDTTKDANLKSIFGKMA
                     EEEKKHKAFLEECLTGAKALQFDEVADYKVSQTVDKPQLSMSLKPADAIALAMKEEEE
                     AMVMYQALADSSKDPDQKKMFQSLSAMEKGHKANLENIYTGMAFPEAW"
     gene            complement(25808..26035)
                     /locus_tag="PLM79_04150"
     CDS             complement(25808..26035)
                     /locus_tag="PLM79_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85530.1"
                     /translation="MKETIRVFVDDTPVSLFRGMTVKHALMSRDPSLYRAAMNGEILV
                     TDELGHRMGLEGSLLEGARLHTRRAGPVSSD"
     gene            complement(26032..27609)
                     /locus_tag="PLM79_04155"
     CDS             complement(26032..27609)
                     /locus_tag="PLM79_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOV85531.1"
                     /translation="MFAPLLGCCIAFPGPAREKTRPVRAVGLVLFSFLLLSLFPGVCG
                     AEPVDLTILHVNDLHGHILPFTEKSVHEQEPVSGAAALAAMIQRERERNPEGTLLLSA
                     GDMFQGAPISTVFHGKPVLEIMNHLRFDAMTLGNHEFDWGRDVLGELTTAARFPFLSA
                     NLKDREGRYLPGVMPYALFTRKGICTGVVGVTTRELAFRTKPSNVEDLTIEDPAAVLP
                     SVLQEMKNRGAQLLVVLSHCGLQADMDLAVEVPGIHVIVGGDSHTVLPDPMKVGEALI
                     VQAGYYGMYLGVLDLSFDPSTSRVARYTQKGELKPVLSGPDRPADPQVASMIREYETR
                     FDARFNQAIGQTAVDLVRQRHAESNFGDLVTDVMREATGAQIAFQNGGGIRTDVYKGP
                     ITLGQLYSALPFDNYLVLMDLTGKQVLELLEQSAALAVQILQVSGLTVVYDLSRPPGN
                     RVVEVMVGTRPLEEQAVYRVVTNDFLAAGGDRFTAFKEGKNVTARESLRDVVIDYVAK
                     RSPIAPVTEGRITFKTP"
     gene            complement(28193..28717)
                     /locus_tag="PLM79_04160"
     CDS             complement(28193..28717)
                     /locus_tag="PLM79_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85532.1"
                     /translation="MKRFLPLVCAILLVLTVSWVPSHSFAGETMAYKLQEQASMYSGS
                     GSWKRCAGVVITPPKKGYVVVTASGMAAFDHRDSSLTLTLSTNNGKGPWIFSLSPGTM
                     GLQTYTVRMVFPVTGGTAQRFNLNGASYDGPGGTIKVQTGSITAEFYPSANVTKLVGS
                     QSGESLETSGPVNP"
     gene            29017..30195
                     /locus_tag="PLM79_04165"
     CDS             29017..30195
                     /locus_tag="PLM79_04165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85533.1"
                     /translation="MSPRNRKHLVLVGGGHAHMTTLRDLDQITGVGHRVTLISPSAYQ
                     YYSGMGPGLLSGFYRPGETRVNIRKMAEGRGGDFVPDRVVRVSPRERALYLEGGASVG
                     YDVVSFNTGSRIRDEVAVRNCPRVYPVKPIENLVRARQTLLAEPGPSPWRLVVLGGGP
                     AGTEIAANLWRLCAEAGIPRDITLVAGERLLAGAPPRVRALALESLRSRNIHVLEATR
                     ARRIEPGEIVLETGRILDYDLAFSAVGVRPSPLFEESGLPTGPDGGLLVDSTLRCTAH
                     REIFGGGDCISLRGTTLARVGVMAVRQNPVLYHNLAAALDGRPLKPFRPGGGYMLILN
                     MGDGRGILWRNGLVLAGSWVFRMKDFIDRRFMERFQVSGELQEPFEPEDPLEKIRERR
                     "
     gene            30279..30415
                     /locus_tag="PLM79_04170"
                     /pseudo
     CDS             30279..30415
                     /locus_tag="PLM79_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_258383117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     assembly_gap    30416..30515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30516..30872
                     /locus_tag="PLM79_04175"
     CDS             <30516..30872
                     /locus_tag="PLM79_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV85534.1"
                     /translation="ESLIAAEDRHKSSLLEAYEELRGKKADPGFPASAVRDLGTERWV
                     EGGVRLEEVLEWSKGRPLSEVIEFSMVLEANSYDRYLLLQSNFEESSARNLFSKLARE
                     EKLHLERLTEAFEKLL"
     gene            30954..31481
                     /locus_tag="PLM79_04180"
     CDS             30954..31481
                     /locus_tag="PLM79_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOV85535.1"
                     /translation="MKWVLAVVGLGTAVIAGLAVVLWTGAYNVAASDPHWDVTVTVLD
                     MARRRSVAVRSAVVRVPPLNDPAWIEEGQQTYEEMCRACHVGVGTARSSFAMGLYPAP
                     PVLSAPSFQASLTDGELYWIVRNGFKMTGMPAFEVVLSDEEVWQLVAFVRTLPGLTRG
                     GSLGGPGRTRAEGSP"
     gene            complement(31637..31765)
                     /locus_tag="PLM79_04185"
     CDS             complement(31637..31765)
                     /locus_tag="PLM79_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85536.1"
                     /translation="MPGKVFLEMIRNTGFVRVEQVAETGFDSSPATRGVLIRAEKA"
     gene            complement(31778..32386)
                     /locus_tag="PLM79_04190"
     CDS             complement(31778..32386)
                     /locus_tag="PLM79_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV85537.1"
                     /translation="MEDCLSSRQRDQIHASIRRKYRGVAENPGGSFRYPTGLAGLQAL
                     GYPSQLLDGLPAKVPDFYCGVGNPFSLGNLSTGAFVLDIGCGAGVDSILAARWVGPQG
                     RVLGVDLTFEMLFRARQNRRRSTGENVLFAQASAEALPLPAAAFDVVISNGTFNLVPD
                     KRAALGEVYRVIKPGGRFWVADQVLLGNPPEDLAARIARWAR"
     gene            complement(32611..32889)
                     /locus_tag="PLM79_04195"
     CDS             complement(32611..32889)
                     /locus_tag="PLM79_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85538.1"
                     /translation="MRKQNLLGKLNVSMVCEEPRMADEFFTDSASKYIHKMIGTPLPP
                     DVRASIRSGRASQWRIGQTNVDIRFVCNGSPKLPSYEMKLVLTSSAAN"
     gene            33678..34382
                     /locus_tag="PLM79_04200"
     CDS             33678..34382
                     /locus_tag="PLM79_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HOV85539.1"
                     /translation="MAFRFFAQNKTASVKALLLVLFLASALYVFRHTEVGSYLSPERL
                     THLIHTAGIWAPLAYVLLYGALVCLFVPATLMTALGAAIFGAYWGFLYVWIGAMIGAS
                     GAFFIGRYLGRDFAASLVNDRLRKYDEAIERNGFATVLYLRLIYFPFTPMSFGMGLTR
                     VSFRDYFSGTALGILVGTFIFTFFIGTAKDAWLSGQWDRLADPKLFLSLGLFVFSFFI
                     PRLVQRLKVWRKLHLE"
     gene            complement(34391..36154)
                     /locus_tag="PLM79_04205"
     CDS             complement(34391..36154)
                     /locus_tag="PLM79_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOV85540.1"
                     /translation="MEAERPAKKFKNRLLWVFLLIFFGAVFPPGAAAEYFVIDEFQSH
                     VAVRRDSSLLVQETIQVTFSAQRHGIFREIPFRYSDRYGKTLETPIRVISVTNAQGQP
                     WDYRVSRRGNVVHIRIGDPQAYVAGVQVYKIDYEVENALLFLDDHDELYWNVTGNFWK
                     TSIARVSATVSLPGDLPAQHLWSACFTGVMGSATTDCRAEKQGNGAAFQSAGGLKIGE
                     GLTVRLGWEKGLVAEPSAWKRFWWRFNWRENWVFLLPPAVFLAMFRRWYAVGRDPRVK
                     EAVTVQYNPPQLGGRTLCPGEVGCLVDERLDPRDITSTIVGLAVKGYMKIQEVVREGL
                     IFDTTDYKLLKLKEPDDALGRFEKELMSDLFPFSSTTISVSGLKNNFYKHLKALRQVL
                     YEDLVRMGCFQKSPDQVRTSYVIWAFVMAGVGGAVTYFFTSDFFWKSIVAWILAGGVV
                     LGFSRVMPAKTRSGAAAAMEILGFREFLNRAEKDRIERMGQQDLYSRFLPYAIALDVA
                     DNWSEAFEGVYQEPARWYESSRGIDFSHRRDFHRSINSMTGSLGSAVFSAPRGEGVGG
                     GGLGGGGSSGGGFGGGGGGSW"
     gene            complement(36415..36972)
                     /locus_tag="PLM79_04210"
     CDS             complement(36415..36972)
                     /locus_tag="PLM79_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOV85541.1"
                     /translation="MTTAAILIILVLILLGAIFLYNKLIRLRNTVQSSWSDIDVQLKK
                     RHDLVGNLVETVKGYAAHERSVFENVTKARTQAMQAASPQERAQAEGSFQSALKGLFA
                     IAEAYPDLKANTNFLQLQTHLKDLEDGIESARRYYNAVVRDFNTLIESFPSNLIAAWF
                     RFEKAQFFELEAAAEERAPVKISFS"
     gene            37130..37486
                     /locus_tag="PLM79_04215"
     CDS             37130..37486
                     /locus_tag="PLM79_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOV85542.1"
                     /translation="MQGLLIRWLILTLAIVLSSYLIGGIRVSGFLPAFFAAALLGLLN
                     TFFRPILILLTLPINLITFGLFTLVINALMLKMASGVVPGFQVQGFWAAFWGALVISI
                     VNWLLISLFRDPVIFD"
     gene            37753..38283
                     /locus_tag="PLM79_04220"
     CDS             37753..38283
                     /locus_tag="PLM79_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85543.1"
                     /translation="MSRRRAVVVLLVVVLTGSLALEALAAERMAFRQDSIRKLTSGSR
                     VWKNVASVPFTPPEDGYVVVTASGMVGFDAEVSSLTLSLDTKPAVRGPWVFTLTPGYQ
                     LFQTYSVRYVFPVKTGVKNTFYLNGLSWDGFGRSISIENGSITVEFYASGNVRAFGVE
                     AMKSVQAAPGDERGNE"
     gene            39020..39727
                     /locus_tag="PLM79_04225"
     CDS             39020..39727
                     /locus_tag="PLM79_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV85544.1"
                     /translation="MIGGTVMSVLWNGTNDLPGLQQIAGAFAELLGNGVLFKYSIASL
                     FRVTAGFYLAVLLAVPLGLFLGRRPSIQRLGNPLIQFLRPVSPLAWVPFALLWFGIGD
                     QPAVFIIFVACFFPILLSTIEASAGIRPIYFHIAANLQLSRWETLSCLILPAAAPSIV
                     LALRVTLGIAWMVVVAAEMIAVKSGLGYLIVDARNALRMDYVVVAILTIGAIGLLLDG
                     VMRRLAQIESVRWRVGQ"
     gene            39753..40535
                     /locus_tag="PLM79_04230"
     CDS             39753..40535
                     /locus_tag="PLM79_04230"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85545.1"
                     /translation="MEYGTGKGCRRSWFGRGKSAGGTVAPSTRLVLDDISLEIERGEF
                     VSILGHSGCGKSTLLKVVAGFTQPVRGRVLIDDQKVKGPRSDHVFVFQEGALFPWLTI
                     LENVSLALRWVKDSVEKKNKAREYLDLVGLEGTADYYPHMLSGGMRQRAEIARALAAR
                     PDILLMDEPFSGLDYLTRLHLREEMLYLQVMLPETTILFVTHDIDEALQLSKRLIILS
                     EPPARIKCCRTIEAPHPRSLSSPMLSTLRTQIYHHLGVHAAL"
     gene            40532..41563
                     /locus_tag="PLM79_04235"
     CDS             40532..41563
                     /locus_tag="PLM79_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV85546.1"
                     /translation="MKVLSFRGSSASFKAFILALLWLGVISLLHASVNREKTASQKVR
                     MGYMPVISNLAAPLVDAATAGSPVHFEALKFGSFAEIAEAFRSGHIQAAFIIAPLAVA
                     LSSQGVPLKIVYIGNRHESTLVVRKDESAASLAEMSGKTLAVPIRYSGHLLAVKRYLR
                     HHGMDPRAVRILEIPPPDMPSALVTGDIHGYFVGEPFASRSIQSGVGRRLLDAEEIWP
                     GFVCNVLIVRDELIRSHPERVGDLVKGCVRSGIRVRENFEETLGILSRYWGQDPQFVR
                     YVFENPPNRFRYDLCVPVRSEIEELVREMETEGLISASSEVGDFLEDRYARAAVEGLR
                     EPLLGLTKR"
     gene            41637..>42578
                     /locus_tag="PLM79_04240"
     CDS             41637..>42578
                     /locus_tag="PLM79_04240"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HOV85547.1"
                     /translation="MNILIVGLDAQIPVIEKLSAVMEDLSVLDGLETDCRPGGSSEVD
                     ALIGSVSEKAGPGNLDLLVSWRTHPHTYLVPCWIAGPAPTRETGCLWPKLTIDRFEET
                     VDLPGLQHWLEEISHWQQNRMLLAPFNTFDKCSPLEIASSLALRKATGRLLTFDDENH
                     EGTLFLREGRVTGAGVKHLRGEEAFHEFLSWSEGSYSWDPERIGEDNMEPRLLEELIR
                     EGLALFREANLFYPLVRDWGRCLRPTGSQSALDDAAVPIYPAQRELYKLIDGRTTAGG
                     IIEASPLSRPRTLSCLAKWLSMGDIEEEPRDAHPSICR"
BASE COUNT         8596 a        12329 c        13243 g         8310 t
ORIGIN      
        1 cggggtccgg gtgttcattt cgctccgtgt tctggaccgg gtggagtgtg gatggtccct
       61 cgcggtcgat gagaccttgg aatcctaatt ggaatcccaa gaagtatagt acagacgcca
      121 gggtcaagga aacgacgaaa tgaaacgaga cgggttgcac tcgggtccgg gatgcggcga
      181 gccgacgggg atctcgattc cgccctctca agaaactcga tttggcaaag gaggtccgtt
      241 agaatgacgt gttgatgtcg agtatgccga cggaaaagat tcctgtgaaa cgggaggagt
      301 tggattgtcc cgggagaagt tcgtccgcct gcgcggggtt cgccagcaca acttgaaaaa
      361 cctggacctg gatatccccc tcaatcgatt gattgcggtg accggggtga gcggttcggg
      421 aaaatcgagc cttgccctcc acaccctcta tgcggaaggg caaaggcgct acgtcgagac
      481 gttttctccc tacgcccggc agttcctgga acgtatggac cctccggacg cggaccttat
      541 cgagggaatt cctcccggca tagccatcga atcgggcacc gccgtccgga gctcgcggtc
      601 cacggtgggc accatcaccg agatcaacga tttcctcaag atgcttttcg cgcggttggc
      661 ggttccccat tgccccaact gcggccggcc catccggcag gacacccccc aaaccatcta
      721 ccggagcctc gaggctcttc ccgaaggcag tcgcctgctc ctttgcttcc cctacgtggc
      781 ggaaaagtcc gcggtgtggc cccggcatct catttccaag gggtttctgc gtctctacac
      841 ctcggggcga accgtggacc tggaatccat ggacaagaaa gccgtcgggc gggggtcggg
      901 aaaagagcac ctggtcgtgg tggaccgtat tccgtggggg aaggtccccg aggaaaggat
      961 catggactcc atcaccaccg ctttcaccga ggggagcggt cgcatggcgg cggtggtcct
     1021 tcccgaggag ctcctctggt tcagctcgcg cctcagctgt gcggactgca agccggaggt
     1081 gagcgtcccg cctccttcgc ccaacctttt ctccttcaac agtcccctgg gagcctgtcc
     1141 cgaatgccgg ggcttcgggc gaaccatcgg cgtggactgg gacctggttg ttcccgactc
     1201 gaccctgtcc ctcgatcagg gagccgtaaa accgtggggg acggatcggg aggaataccg
     1261 agaactgctc gaattttgcc gaaaggaagg cattcccacg gatgtcccgt tcggggcgct
     1321 tagcgaggcc gaccggggaa agattcttca cggcaccccg gattactacg gagtccgggg
     1381 tttcttcgag tggctcgaga ccaagaccta caagatgcac gtgcgggtgt ttctttcccg
     1441 gtaccgatcc tatgaacgct gcaagacgtg catggggacc cggtatcagc gggataccct
     1501 cttgtaccgg ttgaaggggg ccaccatcgg cgaattctgt tcctggagca tcgagcggtg
     1561 caggcaattt ctgaaggagt cgtggcccga gccgaccgcg gatgccgccg ccgatctgct
     1621 ggtgcaggag attcgcagtc gcctggattt tctgcacgcc gtagggctgg ggtatctttc
     1681 cctggatcgc caatcgcgga ccttgtccgg gggggaggtc cagagggttc atctcactcg
     1741 agccctggga tccgccctgg tgaacgtcct ctacgttttg gacgaaccca gcgtgggtct
     1801 tcatgcccgg gaccagggac ggctcatgac ccagttgcgc cgcctggtgg acatgggaaa
     1861 cacggtggtg gtggtcgagc acgatccgga gatggtccgg ttctgtgatg aagtgatcga
     1921 cctgggacct gcgggggggg aaaagggagg cagggtcctt ttccaggggc ctccggaaga
     1981 tctgcctcga tgttccgaga gcctgacggg acgatatctg ggaggtcccg gggccgcgcg
     2041 gatgccccgg gcaaggagga cccccgacgg tcgtccggcc ctcaccattc gcggagcccg
     2101 ggagcacaac cttcgggacg tgacctgcac cattcccctg ggcctgctgg tgggcgtcag
     2161 cggggtttcg gggtccggca agtccaccct ggtggaaaag accctctatc acaactggct
     2221 gcgccggacg ggccgttcca ccgagacccc tgggatctgc gacggcctcg agggggccga
     2281 gtccgtccgg gagatgatcc tggtggacca gcaacccatc gggcgaacgc cccgagccaa
     2341 tctccttacc tacacggggg ccctggaccc cctccggaaa ctgctggccc aaaccccgga
     2401 agccgtggcc cgggggttgt ccacccggta tttctccttc aacgttcccg ggggacgatg
     2461 cgaatcgtgc cggggggagg gtttcgagcg aattgagatg cagttcctgg cggacgtgac
     2521 cgttcgctgt ccccagtgca acggccggag gttcaaggac gacatcctgg agattcgcgt
     2581 tcgaggcctg tccatcgggg acatcctcga gtgcaccgcc caggaggtcc tggatcggtt
     2641 cgcggatgtg gaaccgctgg tgcgtgccct gcagccggtg gtggccatcg gcctcgagta
     2701 ccttcgactg ggacagcccc tgagcagcct gtccgggggg gaagctcaac gcctgaagct
     2761 cattcgctac ctggtctcca acgggccctc gagcctttct aagaatgcgc acaagctatt
     2821 cctcctggat gagcccacca ccggactcca ccctcacgac ctccaaaagc tcctgcgagt
     2881 gctcgatcgt ttggtgaacg acgggaatac cgtggtggtg gtggaacaca acctggatct
     2941 cctcgacgcc tgcgattgga tcctcgagct gggtcccgaa gggggagagg agggcggccg
     3001 aatcgtggcc gtgggctccc ccgaagacat cgcccaaaac cccgacagcc ccacggggca
     3061 gttcctgaaa cgaaggcacg aaggggcaat ccccgccccg gccggggaag gcggtccttt
     3121 catcccctcg gggtccgccc aaagatcgcc ccccgccgtc ccgtcggccg aggatgccat
     3181 catggtgcga ggcgcccggg aacacaacct caagctgagc cgcctcgatc ttccccggga
     3241 aaagatgacc gttcttacgg ggctcagcgg gtcgggcaag tccaccctgg ccttcgacgt
     3301 gctttttgcc gagggccggc gccgctacct cgaatgcctg tccacctacg tgaggcagta
     3361 tttcaaggtc ctcgagaagc cggacgtgga ccaggtcctg ggcctgcccc ccaccgtggc
     3421 catcgaacag cgcaccagca ggctgaatcg acgttccacc gtggccacca ttacggaagt
     3481 ctatcatttt cttcggctgc tctattccaa gctgggaaga caacggtgcc ccgactgcgg
     3541 ccgggaactg acggccctgt cccatgatcg aatccttgcc ctggtggagc gcgagatggt
     3601 gcggggagag gccaaggtcc tggctcccct ggtgcggggc agaaaaggga tctatcgaga
     3661 cctctttttg cggcttcgca aaatggggtt tcagaccgtg cgcgtggacg ggcagtggaa
     3721 ggacctggcc ctcgtgccct ccctcgaacg acaccgggaa cacgacatcg aggtcctcat
     3781 cccccaccga acaagtgcct cgagtcctgc ggcggagttg agctcggccg tgcgaaaggc
     3841 gttggcgctg gggggcggca ccctccactt ggcgggggcg gaaacccggg tcttgagcga
     3901 tcggctctac tgccccacct gcaaccgggg gctcgctccc ctggaccctc gcctgttttc
     3961 cttcaacagc aggctggggg cctgcccctc ctgtagcggc ctggggattg tggaaaagat
     4021 caacgaggaa cgactgttgg gccctcctca cctgggcctt cgggaagggc tgtgggattt
     4081 cctccagacc tcggtttttc gcggaaggta ccgttcctgg ggggcacggc tgcaccaata
     4141 ctggacccaa gagcttcaat tgaacctgga tcgtcccgtg ggcgagctcg aacccgacct
     4201 gaggcacgcc atcctctacg gccgtgacaa aggctctccc gggctcctgg ccgttctcga
     4261 gaaggggcct tcggacgaga agatccaaaa ggccctggcc cccttccgag aagaggcgat
     4321 ctgccccgaa tgcgggggag cccgcatcaa ccgccaggcg cgatccgtcg atttcaaggg
     4381 gcggaccatc accgacctgg tggatcttag catccgggat ttccaggacc tgtggaaatc
     4441 cgtcgacctt ggggaaacgg agcgccccgt gggcctgccc atcctgaggg aaatcctgac
     4501 aaggatctcc tttttgagag aggtcggact ggattacctg accttgaatc gatccgggga
     4561 taccctctcg gggggcgaag cccagaggat tcgactggcg gctcagctgg gatcaaacct
     4621 gctgggggtc tgctacatcc tggacgagcc gaccatcggg cttcatttca cggacaacga
     4681 tcggttgctg aacagcctcg agaaactcaa ggacaagggg aataccatca tcgtggtgga
     4741 acacgacccg gaaaccatgc gccgggcgga tgtgctcatc gagttgggtc ccgcggcggg
     4801 aaatgaaggg ggccgattga cggcccgggg aaacttccaa agcctgtgcc gggatgaaga
     4861 gaccctcacg ggccggtggt acgggacatc ggtggatcac ttgtacgggc tgacccggcg
     4921 aaggcccttt gccccgacgg ggtggatcga ggtgctgggt gcggcggagc ggaaccttaa
     4981 ggggatcgac gtgaggattc cccggggggc gctcacctgc gttacgggcg tgtccggggc
     5041 gggaaagagc accctggtcc acgaggtgat ctaccgcgtg ctgtcccggc ttctgggaag
     5101 agacaacggt caaggcgcca acatccgggg tgagttgatg gggatcgagg ggattcagcg
     5161 cgtgctcgaa gtggaccaca atcccatcgg gaggaccagc cgatccattc cggccaccta
     5221 tgtgggggtg tgggacgaaa tccgccgcct ctatgccatg ctgcccgatt cccgggtgag
     5281 agggttcacc ccgggtcact tttccttcaa cgtcaaggga gggcgctgcg agaactgcaa
     5341 agggcagggg cagctcaagg tggaaatgca ttttctgccc gacgtgttcg tgccctgcga
     5401 atcctgcggc gggagacgct tcaacgagga aaccctggcc gtgcgcctca agggcaaaaa
     5461 cattgccgag gtcctggcca tgaccatcga ggaggcctcc cggttcttcc tggcctttcc
     5521 cggcatttcc cgctctttgc ggatcctgaa tgaactgggg ctcgggtacc tgaccctggg
     5581 ccggcccagt cccaccctgt ccggcggaga agctcaacgg atcaaactgg ccggggaact
     5641 gggcaaccac cgcggtccca ccctgtacgt cctggacgag ccgaccacgg gactgcatcg
     5701 ggcggacgtg aagaagctgc tcgaggtcat gtgggccctc acggaccgcg gccacacggt
     5761 ggtggtcatc gaacacaacc tcgattttgt ctgggcctcg gactacgtga tcgacctggg
     5821 acctggaagc ggggaccggg gaggacggct ggtcgtggcg ggaacccccg gggaaatcct
     5881 caatcacggc gccgtatccc tcacggcccg ggccctggaa aagcacctcg aggcggaagc
     5941 tcgagggtgc cgggccggat gaaatccgca gcaggccgga ttggaccgaa aggacggagg
     6001 atcgagtgcc cgaacggggc tccggacggg gattggatgc ggacggcggg gcggaagcag
     6061 tggaaacggg ggcgggaaaa cccgccccct gtcggtcttt agtgggagtt tacgaagaaa
     6121 cgattgaagg cgttgagata ggcccgggcg ctggcctcga tgatgtccga actcgtaccg
     6181 atgccggaat agacggatcc ggcgatctcc acccggacca tggcctcccc cagggcgtcc
     6241 ttccccatgg tgatggcctt gaggtcatag tcgcgcaaca cgccctgccg gccgacgatt
     6301 cgctcgatgc agttgaacgc ggcgtccacc gggccgttgc ccgtggcggc gtccgtgatc
     6361 ttctcgccgt ccttgacgag ggtcaccgat gccaggggaa ccatggtgtt gccgctcatg
     6421 atctgcaggc tgtgaaagga aaaggtctcg gccaccctgg tgagttcgat ctcgacgatg
     6481 ctcctgagat cggcgctgcg gatctccttc ttccggtccg ccacggagag gaagcgctgg
     6541 tagacccgct cgaactgctc ggggcttacg ctgaaaccca gctcgttgag ccgatggcgc
     6601 agggcggccc gcccggaccg ggccgtgagg acgatcttgt gctgacgaat ccccacatcc
     6661 tcggggcgaa tgatctcgta ggtggcccgg tccttgagga ttccgtcctg gtggacgccg
     6721 gaagaatggg cgaaggcgtt ggaccccaca atggccttgt tgggttggac ggggatgttc
     6781 atcaagcggc tgaccaggcg gctcgtcggg taaatctcct gggttcgtat cccggtgacg
     6841 gcgtcaaagg tgcggggccg cgttctcaag atcatgacga tttcctcgag ggaagcgttt
     6901 cccgcccgtt ctccgatccc gtttaccgtg cattccacct gtcccgcccc gcagcggacg
     6961 gcggtgatgc tgttggccac ggccagtccc aggtcgttgt gacagtgcac gctgagaacg
     7021 acgttgtcca gggcggggac ccgttcccgg agccttcgaa tgaggtcgcc gaactgctcg
     7081 gggacggcat atcccacggt atcggggatg ttgatggtgg tggcccccgc cccgatggcg
     7141 gattccacca cccggcacag gtagtcgaaa tccgtccggg acgcgtcttc cgtcgagtac
     7201 tcgatgtcct cgcaatagcg tttggcgtag cgcaccgcgt ttacggccat ttcgatggcg
     7261 ctttcccggt cccggcgcag cttcttctgg agatggatgt ccgaggaccc caggaagacg
     7321 tgaatccggg gccgttccgc gtctttcacc ccttcccacg cgatgtcgat atcctgctgg
     7381 acggcacggg ccagggccgc gatcaccggt cccttgacct gctcggatac ggccttcacg
     7441 gcctgcaggt cttcgggaga agaacaggga aacccggcct cgatgatgtc cacacccagc
     7501 ttggccagct gctgggcgat ttcgagtttc tgccgcttgt tcagtttggc ccccggcacc
     7561 tgttccccgt ctcgcagggt cgtgtcgaaa atcaagatcc ttttactcat ggtggaatcc
     7621 tcccttggtg gatggatggt tttgggccct cggggccttc ccgggggaag gccgtcgggc
     7681 gaatcagacg aatccggcag gccatttaac cccactcttc tcacctcctt tcgacgctcg
     7741 cactttcatg accgtcgaga ctgtccggtc aggacggttc aaaaacaaaa aggccatggg
     7801 cgggaaccca tggcctttgt tgctgctcta gcgggactgt cacggcattg gcaaaggggc
     7861 gtcacgaggc tcccacaccc gggacccaag gctaaggagg agtaacaagg gtaatagatt
     7921 catgcccgtt gccgccacgg ccgcatcctc aaatcatcag cccgatcgca aaacacagag
     7981 cgatttccac cgcaaacacg gtgatataaa aggcatactt ccttgcggga aaatcctcga
     8041 ttatgcggtt ggcttcttcc atgacctgca aagcttccat gttctgcacc ctgcctttgg
     8101 agtcacggtt tttgttttat gccgcaagac ttcccttcgt ggggtattat tatagtgacc
     8161 ggggtccggc agatcaacca aaaacatgga acgatccgcg gtcgggtgtt cgtgacccat
     8221 acggcgactc ggcctagacc ttcccagggt gcagacaaac cggcacggac gagcgaatcc
     8281 ggccgcaggg caggggaata ttcttgaatc acgggaaatc caccagtagg agcgcaaaga
     8341 gtcctcaggg tgagaagaac accgggggcg gaaagagagc cggactcttc gagactttcg
     8401 acgttcggac ccaggcagag ccctcatccc tcggtcctgt ccccgccgac gtcctggatc
     8461 tcatggaaag gctccgggca aagggccatg gggtgtgggt cgtggggggg gccgtgcggg
     8521 accttctcac gggagtcgct ccccgagact gggacgtcgc caccgacgcc gaaacccggg
     8581 agatcctcga tctgtttccc aaggtgatcc ccctgggcct tcgctatggg actctgcagg
     8641 tgcatacgtc ccaaaggcag gtggagatga cctccctggc aaccgcggcg gatggaaaca
     8701 tcttcaagga cctggcccgc cgggatttca ccatcaacgc catggccctg gttttcccca
     8761 cgggggaact cttggatccg cataaaggaa aggccgacct gggaaagaaa atcctgcggg
     8821 cggtgggaaa tcccacggcc cggctgatgg aagacccgct gcgcatgctc agagcggcac
     8881 gcttcgtgag caccctcgga ttgcgccccg caccttccat ctttgcggcc tgcagggacc
     8941 aggtctatgg gttggacagg acggcccggg aaaggattcg ggaggagttc tttcggatcc
     9001 tcgtggggac ccgggtcgtc caagccttcg aatttctgag acgcacggga atcctggaga
     9061 aactgcttcc ggaattccgg ctggacgggt cgttccgggg gggaaagagg ctctttcttc
     9121 gcaactaccg gcatgccgtg gggaccgtgt ccttatgccc cctcgatggc cgggttcgcc
     9181 tggcggcctt ctttcattgc ctggggaatc cctgccccgc ggtgaacggc gacggcttgg
     9241 gggaatggat cgaggagcgc gacgtcaccc tgtccgccgc ctcccgggtc atgacccgct
     9301 ggaagacggc ccacaaggac atgcgggagg tcttgcgcct tcttgaacat tggattcccc
     9361 cgggcggcga ggatttgagc ccccccgccc tgcgcaggct ggtgctgggc gtgggagagg
     9421 aactgctcga agaccttgtg cattttggat atgcggatcg gagagacgcg gggaatgagc
     9481 gatgcatccg cgctttcgat cagctggttt tgcgcatcaa ggagcagatg gacgggggtt
     9541 ggattgtggg aagatcccag ttggatctct cgggcgagga catcatggaa acgctgggaa
     9601 tcggtcccgg gccccgggtc ggcgagtttc tcaaacggct gcacgacttc gtgatcgagg
     9661 atcctcgggc gaatcaccgg gaaatcctgc tggagaagct gcggcaaatc tccccgaaga
     9721 ccggacctcc cgccgagggt tgtccagggt gaggaggcga gccggcccga cgccctacgg
     9781 tgtatttcag agccgaatgg gactctcacc ccgggttctg cggtcttccc gcagaggctg
     9841 ggatgggcgc cccaaggcct gaaaatcggg cagggggaag atctcccggg aggtcccgca
     9901 gggtacggtt tttgtgcgga tttgggagcg gcggcggtgg tgaagaactg gcaaaaggca
     9961 ctggaagctg atattatgcc ttgagcgtac aaggcaaatg ggttccatgt gcatccggaa
    10021 ttgtgtgcgg cgaaagcgcc gggccgatcc ctcttcccga gggggaaagt ccgtgggccg
    10081 ggcggctttc aaccgccccg gtcccctttg ccccggagaa gagacccttg ctccatagga
    10141 tctgggaaac tgcgacattg cacggcagcc ggtgagaacg gctcgattgg tcgggcggat
    10201 atcattcagg agggcgagac atgctggtga agaattggat gagcaagaat gtgatcacgg
    10261 tcggggaaga ggattccatg cagcaagcca tgagcctgct gaaagagcac aagatccgca
    10321 tgcttcccgt cgtaagggaa gggaagatcg tgggaatcgt ctcggagacg gatctcaagc
    10381 gcgcctcggc ctcggacgcc accctgctcg atgtccacga aatgctgtac ctgatctcca
    10441 agatcaaggt caaagacatc atgaccagac aggtggtcac ggtgcacgag gacctgacgg
    10501 tggaggaaac cgcggaggtc ctccgtgaga aaaggatatc cggagcgccg gtggtggacc
    10561 acggcgggca actggtgggg atgatcacca aggacgacct gttcaacgtg ctcatcgcgt
    10621 tgagcggatt gggcaagaag ggaatccagc tggcctttcg ggtggaggat cgccccggtt
    10681 ccatcaaaga cctgacggac atcatccgac agcacggatc gaggctggcg agcatcatga
    10741 cctcgtatga gggggtgccc aagggattcc gcaacgtcta tgtgcggatt cacgacgtgg
    10801 accgggagga gttggcggtc ctcgaaagag aactgcgtga aaaagccacc ctgatttacg
    10861 tggtggatca ccgggaaaac aagcgcagga tttaccaccc cgagctggag taacgtcggc
    10921 cgggtagttc cgaacaggag tttcgcaaaa gaagaacccc cggttccctt ttcacagggg
    10981 cccgggggtt tcttgccatg ggggtctcaa gggccgacgg gcttcaaatc cccaggaacg
    11041 cctgcttgac cttttcgtct tccaggaggt ctttgccctt tccttcgagg acaatgcgcc
    11101 cgttttccag cacgtaggcc cgatcgcaga tggccagcgt ctgcttgacg ttctgctcca
    11161 ccatgaggat ggtgagtccc tccgagtgaa tgcggcgggc cagctgaaag atctcctggg
    11221 taagaagggg agacaggccc agggaaggtt cgtcaaacaa aatcatcttg gggcgggaca
    11281 tgagaccccg ggcaatggcg cacatctgct gctccccccc cgaaagggtc ccggccattt
    11341 gcttgtgacg ctcccgcagc cggggaaaga gggtgaaaac ccaatccagg gtctcggacc
    11401 ggcgggcttt ggctgccggg gtaagggacc cgagctcgag gttttccttc acgctcattt
    11461 cgggaaaaag ctgccgtgct tcgggaacat gggcaatccc cgcctcgatg atcttgtagg
    11521 gcggcaaaac gtccaaccgc cggtcttcga acaggatctg tccccgggtg gggcggatca
    11581 ggctcgagat ggtcttcatg atggtgctct tccccgcccc gttggcaccc accaggacca
    11641 cgaattcgtt gcccttgacc tcgaaggaaa catcccacag gacctggatg tccccgtaac
    11701 ccgcatcaac gttttcaacc cgcagcatgc tctcattcct ttccgaggta ggcttcaatg
    11761 acggcctggt tgttggcgac ttccgccggg gttcccaaag cgatttcctg gccgtaattg
    11821 atgcagagaa tccggtcgca caggttcatg atcacgtgca tgatgtgctc gacgatgatg
    11881 atcgtcttac cggtatcccg cactcggcgg atgagctcca ccccctcgcg ctgctcctgg
    11941 ggagtcagtc ccgccatggt ttcgtcgagg aggatgagtt cgggttccgt tgccagtgcc
    12001 cgagcgatct cgagtctctt gcggtccccg atggtcagcc ccttggctgg atagtcgagc
    12061 ttcttgctga gctcgcagaa ctcgaggacc tcgagggcct tctcccgggc cgcctgcatg
    12121 gattgacggc gattgaaggc ggaggtcatg acgttttcga gcaccgtcat ccggcgcaaa
    12181 ggcttcacca cctggaaggt cctcccgatt cccagcttgc aaaccttcca cgtgggaagg
    12241 ccgtcgatgg tggaaccctt gaaccgcatg gtgccggacg tctggggata gtagcccgta
    12301 atcaggttga aaaccgtggt cttgccggac ccgttgggac cgatcaggcc gaaaatctgc
    12361 ccctgctcga cctggaagct gaggtcgttg accgcaatca ggccgccgaa attcttcgtc
    12421 aaatgttctg tctggaagta agccatcgtt cgttctcacc catggattag gaatatgcct
    12481 tttctcgtgt ccacaatctg cggatcttcg gccaatctcc gatgaccccg ttggccaggt
    12541 atcggatgac gaagatcacc aggagaccga aagccagtac gtgggactcg ctcacccatc
    12601 tcgggaaaag gccgaaaaag gagctccgga aggtctcctg cagaaagacc atgatgaagg
    12661 ctcccaccgg gggtccccag atggtgccta ctcccccgat gattcccacc aggatggcca
    12721 tgatggaaat gtcatgcagg ttgaagacca cgtgcgggtc gatgaaggcc atgtagttca
    12781 tatagaatgc tccggccagc cccgtaaaaa aagagctgat catcaaagag aggcttttat
    12841 atcggaaggc gctgatcccc atggactccg ccgcgtcctg gtcctcgcga atggcgacga
    12901 aataatagcc ccacttggaa taaaccaccc ggtgaatggc gaacatgcac agggcggcca
    12961 gagcgacccc caggtaataa tagggaagtt tgcttcgaaa ggtctgaatg atgagaatcc
    13021 cctccatccc ttcggtaaaa ctcctccagt caatagccac caatcgaaaa atctctccca
    13081 ccgcaatggt ggccagggcg aagtaagggc cccggaggcg gaagcagacc cagccgatga
    13141 aaaggcccac cagggtggaa acgggtcccc ccagaagaat cccccaccac gcggaaatgc
    13201 ccagcttggt cacgcacaaa cccgcggtgt aggctcccgt gccgaaaaag atggcgtgcc
    13261 cgaaagaaac ctgcccggtg aatccggcaa gaaggttcca cgaagacccg atgaggaccc
    13321 acatgagggc aatgatgaaa atatgccggt agtagtccga cggaaccagc acgggaaatg
    13381 ccagaaagaa aagaacgatg cccaacaaca cccaacgatt tatcctcata acgtaccctc
    13441 tactaaacct tcgtcagcgt cttcaagccg ccgggcagaa agatgagaac gagtagaaaa
    13501 atcaccagac ccactgcgtc cttgagggcc atggaaatat acgtggcacc caacgattcg
    13561 gcgactccca gaacgactcc gcccagaatg gccccaatgg tgctgcccat acctcccaac
    13621 atggtgatga cgaaagcctt gagagtgaac gggcccccga tgtccgggaa taggtagtag
    13681 atgggaagga gcagggtccc ggccgccgca acgaggccgg acccgaggcc gtaggtgagg
    13741 accgtgatcc agtgggcgtt gatccccatg atcatggcgg cgtcggcatt ctgggccgtg
    13801 gcccgaacgg acttgcccag gtccgtcttg agcaggaaga agaatagggc cgccgtgagc
    13861 agcagggcga tcccgaaggc gaccaccatg ggaaagttga gggaaatcgg cccaatgtac
    13921 aaggccgtgg cactgtaggc ggtcttcacc gaccggtagt cggaacgaaa gatgaaccga
    13981 atgatctccg tcaacaccat cccgatgccc accgtcatga gcacctggtt ttcgggaagg
    14041 atggaatcca ccttgagcag agggttgagc aagtatttct gcaggaagat tcccagaaga
    14101 aacagggcgg gcatcgagac cagcagggag aaatagggat cgaggccgaa atactcgaaa
    14161 agcacgaatg taatatacat ggcaaccatc atcaactggc cgtgggccaa gttgatgatc
    14221 ttcatcactc ccatgatcag ggtcatgccg atgccgatga gagcgtagat gccgccgatc
    14281 aaaaccccgg atacgagggt ctgtacgagg atttccatgg gatccagtcc ttttcaaaac
    14341 aaaagtgggg ttgggagcac gcctccacaa ccccacgccg attccacgat tacttccagg
    14401 ttttctccca gtcgatggga aatacaaacg gcttggtggc aacgttcctg ggccatacga
    14461 gctccagttt gccgtcgatc cactggacca ggtaggtggg gagcttgttc tggttggtct
    14521 tcttgtcgta ggagatgaat ttcaccgggc cgaagaccgt catcatgtcc gtggcggcta
    14581 gggaatcccg gacgtctttg ggagtaaggg acttggtcct cttgagggcg tcggcaatga
    14641 cgaaggcggc ggcataagcc tcggcaccgt gatattccgt gtccttgttg aacttcttga
    14701 cgtatttatc atagtaggcc atggcccccg ggtaagggag ggtctggtac cacagtgtcg
    14761 ccgagtagac catttccgag gccttgccgg cattcttcgg gaattcgggc agggtgaatc
    14821 ctgcggcccc tcccacgaaa accttggggg acaagcgcag ttcccgggac tggctcatga
    14881 gcagggatgc gtccatgatg taggagatca tgtacaccaa atcggggttg gcctgtttga
    14941 ccttgaccag gaggggtttg aagtccacac cccccttgga atagccctcg ttcaggagga
    15001 ccttgattcc caggcgattg caggtttcct ggaaggtctt ggaacccccg gacccgaaat
    15061 tgctgtcctc gaaaaggatg gctgcggtct tgggcttgac cacttcggtg agaaaggact
    15121 cgagggcgga cggatattca ctgacggcag ggttgagccg gaaaacatag ttccagtttt
    15181 gttcggtgat tttgtccgcg gacccggtgt ttaccaggaa gggcatttcg ttctgctggg
    15241 ccactccggc aatggcgaag gtctcggagc tcccgtaccc gccccccaac atgaccacct
    15301 tgtccttggt gataagcttt tccgcggcgg aacgagccac ctcgggcttt ccagtgtcgt
    15361 cctcgaagat gaaatcgagt ctcttgccgt tgacgccgcc cccggcattg atttcctcca
    15421 gggccagctc gaaggagttc ttttcgattt ccccgaaggc agcttggcct cccgtcaacg
    15481 gaaggatgat gcccaccttg atggaatcct gcgcgagggt cggcccggcc gacaaggccg
    15541 cggccatgca tagagcgaga cacacagttg caattctctt catcttgccc tccttcaacg
    15601 gtccgtgaaa agtgtacaac ccattcatcc aggagcaacg tcgacacgct gcaaataagt
    15661 gcgttgaaaa ggcaccgggg atgtggcaga agaggatcct tcggctagga aacctattat
    15721 aaagagggct tgaagtcaag ccaaggattg cggccacacg cagaatcccg gggaggaagg
    15781 aactgaatga gatacgcaat gggcaccaca ccggatcatg agattgttcg ttctattgag
    15841 ccggtggtgt tgcaatcgga acatgtgctc tttcaccccg aagccgtgaa agacgccgcc
    15901 gaccgtctcg aagaacaaga ggacttcccc ggcctttcct ggaaacaccc ctgccatttc
    15961 tttgacggcg gggaggaaac cgctcgttgg atcctggtac tggatgttct gaaccattgt
    16021 ttctggcctt cccccggcca aaagacctgg acggtgatcc acggcggccg ccgttggtcc
    16081 gggtactggg gcctggccgc aagcctcaac cgggccatgg aacgaagaac ccccgtcacg
    16141 cgggcctcct ggctggcctc cctcgaagaa gaggacctgc ggcgggtatt tgcgggggaa
    16201 ggggacatcc ccctttttcc cgagcgcctc cgaaacctgc gagaggcggg ccgcattctc
    16261 gaggagcgtt gggggggaga tttcgtcaat ctcatcgagg aagcttcgaa aagtgccctg
    16321 aaattggttc gcctggtggt ggagagcttc cccggttttc gggaccaggc cctatacaga
    16381 ggatataagg taaatttcct gaaacgggcc cagctcctgg tttgggacct ttactgcgcc
    16441 ttcgagggca aaagcttcgg cgagtttcat gacctggagg ccctgacggc ctttgcggac
    16501 tacaagctcc cccaggtgct cagggaactg ggagtccttt cctatgattc gcccctggca
    16561 gcccgagtgg atcgccggga ggagctccaa cccgggagcc acgaagaggt cgagatccgg
    16621 gccctgaccc tttgggccgt ggaggccatc aaggaagaac tgctccaacg aggccggaat
    16681 ctcatgagtg cgcaaatcga ccgggggctc tggaacctgg gtcaacggga cgcctaccga
    16741 cgccgaccct accatcgctg ccggacgacc tattattagg gcgaggggca aggggctgga
    16801 ggcgaggggc aaaatagccg ggaagcgtgg agggccgcag ggtgggcgcg gcgaagcgaa
    16861 gcccaccggg ggaattcgag gctcgatgat ggaagcgtgg gcctgggggc gaggaggccg
    16921 ggaggctcgg gattgcgtcc ctttcccggc tggaaccgct cggtttgacg gaacgacaag
    16981 aaggagacat tcgtggaata ccgttatcgt cgtgaaagtt tcggggaact tccggttcgc
    17041 ccggagcacc tggatatcta tctggatttc cggccggatt tcgtggaagc ggtcaactgc
    17101 atgcggatga cgccccgcgg ccccatggaa gtcctggaac tggacgccaa cggcctggac
    17161 atcctggcgg tggaatggag ggagacctcc ccggattccc cgggcagctg ggaagccctg
    17221 gcctacgaag accgaaggga tcggaacaag ctggtcctct tctttccccg gccggtttcc
    17281 cgagaccgcc ccttgtgggt tcggacccgc acccgctgtt acccgtcgga ccatatcctc
    17341 gagggaatct acaaggacgt gacccctccg ggggcccccc agcaatacat gtcccaatgc
    17401 cagcaatggg gtttccagcg gatcatgccc atcctggacg actgccgtgc caagtgcacc
    17461 atgaccacca ccctcgaggg ggatgcccgg tacacgcacc tcatcagcaa cggcaacgtg
    17521 aacctcgagt ccaatcccca aggggttccg gtccccaagc ccggggaccc ccggcgccgc
    17581 accatcacct tcgaaaaccc cgttcccatg gctccttacc tgttcatcgc ctgtgcgggc
    17641 acctgggaca ccttgacgga ccgggtgacc taccctgacg gccgaacggt gcgactcgag
    17701 tacctggttc cccccgggat gagcccgtat gcccgggttc ccatggaaat cctcaagaag
    17761 gccgttctgt ggatccggga aacccaggat tacacctaca ccggggacac ctaccgcacc
    17821 atctgcatga ccaagtccaa tttcggcggc atggaaaacg tggggaacac caccatcgtc
    17881 acggatgccg ccctggttca cgagcacagc ctggacgcat cgctcctcta tgcccatgcc
    17941 gtcatcgtcc acgaattcga acacaaccag tgcgggagcg aaaccactat ggacactccc
    18001 ttcgacgtgt ggctgaacga agcctacacc gtggatgtgg aacgccgctt tatggccgac
    18061 cggttcgatc cgaccctggt tcgcctgctg caagtggacg gcatccgaaa tcctctcctc
    18121 gggcccctgg ccatcgagga cggagggcat gtgggccggg tggtccgaga aggcttcaac
    18181 gacccggacg agctcatcga cggggtgacc tatgtgaagg cggccgaaat catcggcatg
    18241 cttcggcggg tgctgggaga agaaaacttc cttgccggaa agaccctcta ctttcagcga
    18301 taccggcacg ggaacgccga tacggaccag tttttcgcct gtttcgaaga ggtctccggg
    18361 acttccctgg aaccattcaa gaagggatgg ctctatcgca tcgggtaccc ccgggtgacg
    18421 gcggaaacct ccttcgaccc ggccaagaag gaatttcaca tccggtggcg ccaagaggtg
    18481 ccgccgggag aagccccgtt tcacatcccc atcgagctcg ccttggtggg ggaagacggg
    18541 aaggaccttc ccggcaccgg ccaggtcttc cagctcaacc gggaggaggc atccttgacc
    18601 ctttccggga tcgaggcgga acccgccttc gcttccatga accgggattc ttccttttac
    18661 ggtaccttct cccaccggaa ttccaccccc gatcttttga gaaaacaagt cctgctggac
    18721 cccaacggcc acaaccgggt ggacgccatg cggaagctga cggatcttca gcggattcgc
    18781 ctgctggaag acccccgggg agaaatcgat ccccggtggc tggacctctt cgaagacctc
    18841 ctggcggatt cgaccgtggc tcccggcctg cagtcctacc tgctgcgcat cgaggaacag
    18901 cccgtggacc gcgactacgc cacctggtat cccgaactgg cggcggcccg tgaaaccatg
    18961 atgcgggccc tgaacggccg gtatcgggca aagctcctcg atcggttcga ggggctccgg
    19021 gcggcgccgc ccccgccgga atccgaaccc tggacgggcc tcgaggaacg gatgctaaag
    19081 aatgtcctct tggacctcat cgtcgtggac gattcccccc aaagccacga actgatcctt
    19141 gactattttc gaaacgccgc caaccccacg gaccgggtgg cggccctggg ggcactaaac
    19201 cgctccgggt cgcctcaacg acggccggtg ttggaagaag tctacgaagc ctggcacggg
    19261 catctgagcg gctatgccaa ctaccttcga gtggtggcgg gggggacccg cccggatgtc
    19321 ttcgacctga tcgaccggga aaagcgtcga agtaccttcg acatcaccca gcccacctgg
    19381 tcccgggcgc tgtttgtttc catggccgcc aacaacaaga tgctgtggac ggagaaagga
    19441 atccggtgga tgacggccgc catcatcgaa ctggccccca tcaatcagac caccaccagc
    19501 cggatgctca acgccttcca gcatgtgagg cgcctgcggc cgccactgcg ggagcgggtg
    19561 ttggaagccc tcgaggaaat cgtgtctagg gtcggccggg aggcgagccc gaccgtctac
    19621 gggcaggcga gttcctacct ggcgggaagg tgacgtcggc cccgggccgg gggaaccttc
    19681 ccggcttgcg ggtagacgag cttctccggt ggtgtaggtt cgagtttgtg taaatccttc
    19741 cgggttgcaa aaggaggcgc tcttcctggt gcgggaaatc ccgatcatcg cggcgcatcg
    19801 aattccatgt gatgtccggg tccttgaaac cctgaaggaa gccctgaagg ttcctttacc
    19861 caggggggtt gaaggcggaa gggaagggtg ccggtttgcg ggaacgcgac atctttagcg
    19921 tacctcatag tccatggact ttacaggtat tctgcttcgg cgtatcccga tgggcacgaa
    19981 actcgggtcc cgcccagccg gtccaccgct catatttcct tgacagccgt cttttaagac
    20041 ggtaaaagtc ctggcaagaa aataagctgt ggggccttct gtaaatattg aagatattgc
    20101 caaagtattt gtaccaagaa catatacata agtacaatga cattatgggc cgatacaaat
    20161 actatggagt caggatgagc agaggtctca tagttgcaaa tggagatcaa ccattacttc
    20221 tatcccatgt accggacgga tgaattccga cagatcacag ggtgcggttc atgttgggtt
    20281 ccgtctgtta gatgaacatg tttgccttct atcagtcata ttggcggggt cgtgtgctat
    20341 tcttgaagag atggcggatg aatgggcgac cgagagagaa aggtggacgt ttccgaacga
    20401 aggggcctcc tgcgagacct ctgcagtcat tctgccctca ggagaggtga acgtctcagg
    20461 cgcggcagtg tcagtgggcg caagaggatg aataggctcg ggaacatcca tttcgttgag
    20521 tcctgcgttt ccaagagagg gatccagccg attcaagttc aaagcggata tgcaagcgcg
    20581 agaggtaggc cgcgatttcg caatattcga aggcagacat actctgcggt gtcgcttccg
    20641 ggagagctga ttcttgagta cctgacaagt aaatggagcg gaatgcaatg acccccaaag
    20701 tagtccggtg tctgttggtc gcccttttga tctgttgctg tgggatagga tgggcacaaa
    20761 acatcacgtt tcaaccaatt gtgacccctg ggctgcagca ggagaatcgc tcacaacaga
    20821 ttgtccccca gcagggtacc agcagccagg tcgaccaaaa ccagcaggca ccaccaggca
    20881 cggagggaac tcctacaccc gtgccttctc acgcgccggc tctacaggtc ccttcccaga
    20941 ttcctcaagc gccgtcttcc ttacccgtgt cccttccccc aggggctaca gaacagcggg
    21001 cccccgatcg gagtgttccc tctgcgccag gattgacagt ggaggaagta tcggatttcg
    21061 aaaagtatgt ggcggggaaa tcgccggaga agctctcatt tgatatcaaa cagtttggct
    21121 acgatctttt cctggcgcct ccaaccctta ccgggcccgt tcagcggacc gttcctgttg
    21181 gcccggaata tgtcatgggc cctggggacg aactgcgcgt gaatgtctgg ggaagcatcg
    21241 agggggtttg gaacgtcgta gtggatcgcg acggggccat cactctgccc aaagtaggca
    21301 cactgggcat tgccggtctg agctttcagg aaaccaaggc agtgattcag aaggaactgg
    21361 ccaagcagta caccgacttt gagatgaacg tgagcatggg tcagctacgg ggcatggggg
    21421 tatatgtcgt cggaaaggcc aggaagcccg gcgccttcgt tgtttcttct ctttccactc
    21481 tgctgaacgc gctccaagag tcgggggggc cgagcaaagt cggtacgatg cgcgacatcc
    21541 agttgaaacg caacggagaa accattgccc atctcgacct gtatgatttc ctgctcacgg
    21601 gaaacaaaga aaacgatgtt cgcctcatgc ccggagacgt catcttcatt cccgccattg
    21661 ggcctatggt gggcattgcc ggagacgtca aagtcccggc aatctacgaa ctcaaggggg
    21721 cagccagggt ttccgacgtg atcaagttgg ccgggggcac tactgcaaaa gcgttcaccc
    21781 agagagtcca ggtggaaagg ttctccaaaa ggaccaacaa ggccatactg gatttgaacc
    21841 tcggccaact caagggcaaa aacgacattc ttctcgaaga cggcgacgtg gtgaaggtct
    21901 ttgccgtcac ggagaatgtg accaacagag tcgtactcaa gggaaatgta cggcgacccg
    21961 gagaatacga atggcggaag gggatgagag tccgcgatct tctgaagagt accgaggagt
    22021 tgcttccgga aaccctgatg gagcgcgctc agatcgatcg cctcgtaccc cctgattacc
    22081 acctggaata tgaaacgttc gatttgggga aggtgcttct ggaaggctcc gaaagcgaga
    22141 atctgcttct tgctccctac gacaccgtga tcgttttcaa taagtgggaa gtgaaggaga
    22201 gggataaagt gagatcgtcc ggggcattga acaaaccggg tgagtttgac taccgcaaga
    22261 acatgaggct ctccgacctg atcggacttt cgggcggact caagtattac gcatccgcaa
    22321 aagaggctga gctcacccgg gtgaaaccaa ccccggaggg gcccgtgacc gagaagatca
    22381 ccgtgtaccc atccagtgca ctgcagggaa atccggaggc cgatatcctt ctccaggaga
    22441 atgactatct ctttgtccgg gccatcccgg aatgggacct ttacaagctg gtgacggttg
    22501 gaggacaggt catgtatccg ggggcgtact ccgtggacaa aagggaaaag ctttcctctc
    22561 ttttgactcg cgcgggaggg ttcacggatg tggcctatct ccagggggcc gttttagtgc
    22621 gggagagtgt caaaaggttt cagcaggaac agatcaatcg gatggttgac aagttgcagc
    22681 gagagctcct gtcgagcggc agtgtgaata ttgggactgc atcgacgccc gaggaagcga
    22741 agattttgca ggaacaggag aaacagaaag cacttttcct tgaaagcctg cggcatctcc
    22801 aggcacaagg acgattgttc atcaccctgg acaggctcga taagctcaga ggcagtcaga
    22861 acgatatcga actggaggag ggggactccc tctacattcc caccaagcca gcgaccatcc
    22921 aggttatcgg tgccgtttac aacaaccctt cggcaatgct tttcgagcag gggcgggacg
    22981 tctcgtacta tattcgcatg agcggcgggt tcagtgaaaa cgctgatagc aagaatacct
    23041 atgttttgaa agtcaacggc cgtgccctca aacccaaggg aaaatttctg gcatgggaca
    23101 gggactccaa cagctggagg tcttccgggt acgccgacct gcagccgggc gataccatcg
    23161 tggtgcccga gagactggaa aaagtggctt ggatgcgaaa tataaaagac attgcgcaga
    23221 ttctattcca ggttgggact gcagcgggtg tcgttgtggc ggccggactc tagcgatgca
    23281 agcgagtagg ccccgaagga acatgtggat acagctgtcc ggtccggcag gggccgtgtt
    23341 gggtggatca cagagcggat ttgctgcttt gtcggcaagt ggcgccgcag atcagtagtt
    23401 gaagctctgc agaccttgga ggcgttttgc tggttgcggc gcaaaggggg ggtgcggaac
    23461 cgggcggttc accccgactt ggcgatcccc cttcggtgga tggctcgctt acggttcgtt
    23521 tcctcacagc atcgagatga gggcccttga ggcttaatcc catagtagag agaggaaacg
    23581 ctcatgccgc ccggattctt ccggaggctg tacggccgtt aaggaatatc cgactgggtg
    23641 aaccgcatcc atatcttgtc gaggaaacgg agagactcat ggaaacgtct tgcggttgag
    23701 tgaggtcatt cgtttcgctg gccgggatgg gggatgaatc ttcgggatcc cggagcggct
    23761 tttggcgttt attggggagc tgcatcgcat gaaagaagtg ttgcaggatc aagtggatga
    23821 aattgaagaa gtacatttac tagattacgt gcttgtgttg ctcaagagaa agaaactcat
    23881 tgctgccatt acttgcgcca taagccttct gagcatcatt tatgtcctgg tcattcctcc
    23941 catttacgaa ggaacagcca ggatcctccc cccgtccaag gggggagcct ccatggggct
    24001 gttgagccaa ctttcctcca ttgccggaat ggcgtcagca cttcccggta tgcctggcac
    24061 tccttccgac ttgtatgttg ggatgctgac cgacagccgg accatagcgg atgcgatgat
    24121 tgaccgattc aagctgatgg aggtctatga taaggacaat cgggttgaca ccaggaatgc
    24181 gttatccaaa cgtttgaggg cgatgagtga taggaaaagc ggtatcatta ccataacggt
    24241 tgaagatgaa gacccgaaaa gggccgccga tatggcgaac gcgtttgtcg aggaattggc
    24301 taagatcaac aacacgcttg cggtcgccga tgccaggcaa aaaaggctct ttttcgaggg
    24361 acagttgaaa gaaaccatta cggcactgag ggtcgccgaa gaggacctga agagcttcca
    24421 ggaaaaaacg ggcgccatcc agatcagcag tcaggctgga gcggttgtgg aaggcatcgc
    24481 ctcactcagg gctcagatcg ccgccaaaga gatccagatc aaggtcatga ggacctattt
    24541 cactgagaac agccgggacg tgagacgcgc agaggaagaa ctggccggga tgaaggaaca
    24601 gttgaagaaa ctggaagccg gcggcgacaa gtcctatggc agcacgatta tccccacggg
    24661 ggaagtgccg gctctgagtg tcgagtatct ccggaaactc cgagaattca agttccagga
    24721 gaccctttac gagatgctct ccaaaatgta tgagtccgct aggctggatg aagcaagtga
    24781 tgtatcttcg atccaggtgg ttgattctgc ggtcgtgccg gagaaaaggt ccaagccaaa
    24841 gaggacattg acggtcgtac ttgcaaccgt gctcggcttt ttctttgcgg ttcttgcgtc
    24901 gttcttcgct gagtattggg aaaaaatgtc tcaaagccct gaagactgcc agaggttgga
    24961 gaagatcagg gaacatttgg actcaattag gaacaatcgc gcactgacct ggatctgcag
    25021 agtgttcaaa aggggatccg gagggtccta gcccggccct ttacccataa ataaggactc
    25081 acggagcgca tcgcttgacc tgaggttcat gttcctctat tctgcccgga catgtttcag
    25141 gttcgaatcg ttcgcacact catgccaagg gagacacacg atgaaaacgg aggagtacaa
    25201 gaagatcatt cagatggcga tcgggaacga agttgcagcc tacgagttct acaagagcgt
    25261 ttgcgacacg accaaggatg cgaatttgaa aagcatcttc ggcaagatgg ccgaggagga
    25321 aaagaagcat aaagctttcc tggaggaatg cctcaccggg gccaaggccc tccagtttga
    25381 cgaggttgcc gactacaagg tctcccagac ggtcgacaag cctcagcttt ccatgtcgct
    25441 caagcccgcc gatgccatcg ccctggccat gaaggaggag gaggaagcca tggtcatgta
    25501 ccaggctctg gccgactcga gcaaggaccc ggaccagaag aagatgtttc agtccctatc
    25561 ggccatggaa aagggacaca aggccaacct cgaaaacatc tataccggca tggcgtttcc
    25621 ggaagcctgg tagccgggtc ccaaagcctg cagcgttcga cggcggggtc cacaacgacc
    25681 ctgccgcttt ttctcggggg cttggccacc gctttcgccc acccgtgaac cctcgggatg
    25741 ccgcccggag aacagagccc cgcgccatag ggatccactc ctctttcgtt gagatcgaag
    25801 accgatgtca atcggaggaa accggccctg cccggcgggt gtggagccgg gcgccctcga
    25861 ggaggctccc ctcgagcccc atccggtggc cgagttcgtc cgtcacgagg atctccccgt
    25921 tcatggccgc ccggtaaagg gaagggtccc gggacatgag ggcgtgtttc accgtcattc
    25981 cccgaaagag gctcaccggg gtgtcgtcga cgaaaacgcg aatggtttcc ttcatggcgt
    26041 cttgaaggtg attcgaccct ccgtgacggg agcaatcggg gaccttttcg ccacatagtc
    26101 gattaccacg tctctaaggg attcacgagc ggtcacgttc ttgccttcct tgaaggcggt
    26161 aaagcggtcg cccccggctg ccaggaaatc gttggtcacc acccggtaca ccgcctgctc
    26221 ctcgaggggc cgggttccca ccatcacctc gactacccgg ttgcccggcg gccgggacag
    26281 atcgtagacg accgtcagcc cggatacctg gaggatttgc acggccaggg cggcgctctg
    26341 ttccaggagc tccaggacct gtttccccgt gaggtccatt aggaccaggt agttgtcgaa
    26401 gggaagggcc gaatacagct gtcccagggt gatggggccc ttgtacacgt ctgtgcggat
    26461 tcccccgccg ttctggaaag cgatttgagc tcccgtcgcc tcgcgcatga cgtcggtcac
    26521 caggtccccg aagttggact cggcgtgacg ctggcggacg aggtccacgg cggtctgccc
    26581 gattgcctgg ttgaacctgg cgtcgaaccg ggtttcatac tcccggatca tggaggccac
    26641 ttgagggtcc gcgggacgat cggggccgga caggaccggc ttcagctccc ctttttgcgt
    26701 atatcgcgca acccgggacg tcgagggatc gaaggacaga tccaaaaccc cgaggtacat
    26761 tccgtagtat cccgcctgaa cgatgagggc ctcgccgact ttcatgggat cgggcaggac
    26821 ggtgtgactg tctcccccga cgatcacgtg gattcccgga acctccaccg ccaggtccat
    26881 gtccgcctgc aagccgcagt gggagagaac caccaggagt tgagcgcccc ggttcttcat
    26941 ctcttgcagg acggaaggaa gaacggccgc cgggtcctcg atggtgaggt cctcgacgtt
    27001 ggacggcttg gtgcgaaagg cgagttcccg ggtggtgact cccaccactc cggtgcaaat
    27061 ccctttcctg gtgaaaaggg catagggcat gacccccggc aggtagcggc cctctcgatc
    27121 cttgaggttg gccgacaaga aggggaaccg tgccgccgtc gtgagctcgc ccagcacgtc
    27181 cctcccccag tcgaactcgt gattgcccag ggtcatggcg tcgaagcgaa gatggttcat
    27241 aatctccagg accggctttc cgtggaacac cgtcgaaatg ggggccccct ggaacatgtc
    27301 cccggcggac aagagcaggg tgccttcggg attgcgctcc cgctcccgtt gaatcatggc
    27361 ggccagggcg gcggcgccgc tcacgggttc ctgttcgtga acgctctttt ccgtgaacgg
    27421 cagaatgtga ccgtgaaggt cgttgacgtg caggatggtc aggtctacgg gctcggcccc
    27481 gcaaaccccg gggaaaagcg acaggagcag aaaggaaaaa aggaccaggc ccacggcacg
    27541 gaccggacgg gtcttctccc gggccgggcc ggggaaggca atgcaacacc cgagtagggg
    27601 tgcgaacact ccaaagaaag gactcaacga atgaaacgag ggagagactt cgcgcggagg
    27661 ggccttcccc ggtttttgcc gggggtggaa acaacgagag ggtccgaccc aagtgccgcc
    27721 cctatccgtc tcgccctttc gggtccgcag gggtgttgcg gcggggtttc caaaaagctg
    27781 cctcatggag ggtccttcaa tgtcatcggg gagttccgaa tcgggttcgc ttcaggtttt
    27841 gatcccggac ggaattcgtc gaaagcccgg cgctgggggg gcctaccggg caacatccgg
    27901 cctcacgtct ttctccgggt tccggtgtat gggccggcgc aaggacgaag ggcgtccctc
    27961 caccctgagt tgagcgatcc cgagagcctc agcccgtcaa gcccgcgagg cgggcacccg
    28021 tgggtatgca ccctcttcgt tgatgtccgc ctgggcgaaa atgagcaaac gaaattcgta
    28081 tcggccgggt cgctggatcc acgaccgggc gccggttgga aaaaacccgg ggccgacgcc
    28141 tggggacgcc cgccccccgg gccgggtggg tgatcccttt gcccttcgcc tgttaaggat
    28201 tgaccggacc cgacgtctcg agggattccc cggattggga tccgaccagc ttggtaacgt
    28261 tggcgctggg atagaactcg gcggtgatgg aacccgtctg aactttgatg gttcctccgg
    28321 ggccgtcgta ggatgcaccg ttcagattga agcgctgggc ggtacccccg gtgacgggga
    28381 ataccatgcg aacggtgtag gtttgaagcc ccatagtgcc cggggacagg gaaaagatcc
    28441 agggaccttt gccgttgttg gtgctgaggg tcaaggtaag actcgagtcc cgatgatcga
    28501 atgcagccat cccggatgcg gtaacgacca catagccctt cttgggcgga gtgatgacca
    28561 cgccggcaca gcgtttccaa ctgccgctgc ccgaatacat ggacgcctgc tcctggagtt
    28621 tgtaggccat ggtctccccg gcaaaagaat gcgagggaac ccaggaaacc gtcaggacca
    28681 aaagaattgc acagaccaag ggcaaaaagc gcttcatgtt ttcctcctcc tcttacgtgt
    28741 ctaacccact aggaaagtcc cggtacgacc atcgagacca gcccgtttcc acttcccgcc
    28801 gggagccggt gggaggctca tttcgcggtc cttcccggcc tccgggatga aacccgtttt
    28861 cgatacacct cttggaacca aggaaagtcc cgaacaggac cggggttgac aaatccggtc
    28921 cggaggactt ctttgacgta taccacaggg agaaccgatg cagaaacgga ttcacgatcc
    28981 cgcttcgaga ggcaggaaaa tcccggagaa ttcccgatga gcccccgcaa cagaaagcac
    29041 ctggtcctgg tgggcggcgg ccatgctcac atgacgacgc tgcgggacct ggaccaaatc
    29101 accggagtcg gacaccgggt gactctcatc agtccgtctg cctaccagta ctattcggga
    29161 atgggcccag gactcctgtc agggttttac cggcccgggg aaacccgggt gaacatccgg
    29221 aagatggccg agggccgggg cggtgatttt gtccccgatc gggtggtccg ggtgagtcct
    29281 cgggagaggg ccctgtacct cgagggagga gcctccgtcg gatacgatgt ggtttccttc
    29341 aacacgggca gccggattcg cgatgaggtc gcagtgcgga actgtccccg ggtatacccg
    29401 gtaaaaccga tcgagaacct ggtccgggcc cgccaaaccc tcctcgccga gcctggtccg
    29461 agcccctggc gtctcgtggt gttgggaggg ggcccagcgg ggacggaaat agccgcaaat
    29521 ctctggaggc tctgtgccga ggcgggaatt cctcgagaca ttaccctggt tgccggggag
    29581 cggctgctgg caggggcgcc cccccgggta cgagccctgg ccctcgagtc gctgcggtcc
    29641 cgcaacattc acgttctcga ggccacgcga gcccggagaa tcgagccggg cgagattgtc
    29701 ctcgaaaccg ggcgcatcct cgattacgat ctggccttta gtgcggttgg ggtaagaccg
    29761 tctcccctgt tcgaggaatc gggactgcct acgggacccg acggtggtct gctggtggat
    29821 tccactctcc gatgcacggc tcaccgggag attttcggag gaggggactg tatcagcctt
    29881 cggggcacga cccttgcccg ggtcggggtc atggccgtgc ggcagaaccc cgtcctgtac
    29941 cataacctgg cggcggctct cgatgggcgt ccgttgaagc ctttccgccc cgggggcggc
    30001 tatatgctga tcctgaacat gggggacggc cgggggatcc tttggagaaa cggcctggtc
    30061 ctggcgggtt cgtgggtgtt tcgaatgaag gatttcatcg atcggcgctt catggaaagg
    30121 ttccaggtgt cgggggagct gcaagagcct ttcgagccgg aagatccctt ggagaagatc
    30181 agggagcgga gatgaacaag gaagactatt tcaagccggg ttcttcgtgg tttccggacg
    30241 aagtgcggga cttcttgcgg gagcgggagg ccgggaccta caacctgttg gacgtgcgcc
    30301 agcccgtgga ataccaagag ggacacattc ccggggcaaa gctcattccg ttggggcgcc
    30361 tgacggggca gctcaaggaa ttgactcccg gggtgccgac cattgtctat tgttcnnnnn
    30421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngaaag tctcatcgct gccgaagatc
    30541 ggcacaagag ttcccttctc gaggcttacg aggaactccg ggggaagaag gccgatccgg
    30601 gcttcccggc ttcggccgtc cgggacctgg ggaccgagcg atgggtggaa gggggcgttc
    30661 gactcgagga agtcctggaa tggtccaagg gaaggccctt gagcgaagtg atcgaatttt
    30721 ccatggtcct ggaagccaat tcctatgacc gatacctcct tcttcaatcg aacttcgaag
    30781 aatcttccgc ccggaacctt ttctcgaaac tggcccggga ggagaagctg cacctggaac
    30841 gattgacgga ggccttcgaa aaactgttgt aagaacgggc aactccccgt tgtgtacggg
    30901 tcctcgaagg agtggggaaa tctcttttca agggtcgccc cggaggtgga cggatgaaat
    30961 gggttcttgc cgtggtggga ttggggaccg cggtgatcgc gggacttgcc gtcgttttgt
    31021 ggacgggggc gtacaacgtg gccgccagtg atccccattg ggacgtgacc gtgaccgttc
    31081 tggatatggc ccgcaggcgc tcggtcgcgg tccgcagcgc cgtggtccgg gtcccgcccc
    31141 tgaacgaccc ggcctggatt gaagagggtc aacagaccta cgaggagatg tgccgggctt
    31201 gtcacgtggg agtgggaacg gcgcgaagtt ctttcgccat gggcctctac cctgctcccc
    31261 ccgttctgag cgcgccttcg ttccaggcat ccttgaccga cggcgagctc tactggatcg
    31321 ttcgaaacgg cttcaagatg accggaatgc ccgccttcga ggtggtgttg tcggacgagg
    31381 aggtgtggca actggtggcc ttcgtgagaa cattgcccgg cctgacccgg ggagggtccc
    31441 tcggagggcc cgggcggaca cgggcggaag gctcaccgta accgcggcgg ccaaagggat
    31501 tcctgcgggg aatcgtcctc ccccgaaccg ttcgctccgg ttgggcatcg gatttccccc
    31561 cgggcccccg gtaaatccca tggtgtgcac ggggacccgg gtagtggctg aatttcccgc
    31621 cacgattccg ggacaatcag gccttttccg cccgaatcaa aacgcctcgg gttgcgggcg
    31681 agctgtcgaa cccggtctcc gccacctgtt ccaccctcac gaaccctgtg ttccgaatca
    31741 tttccaggaa gaccttcccg ggcacggctc cgccttctca gcgagcccac cgggcaatgc
    31801 gggccgcgag atcttcgggg ggattcccca gaaggacctg gtcggcgacc cagaatcggc
    31861 cccccggctt gatcacccgg tagacttctc ccagggctgc ccgtttgtcc ggcaccaggt
    31921 tgaacgtgcc gttggagatc accacgtcga aggcagcggc aggaagaggc aaggcttcgg
    31981 cgcttgcctg ggcgaaaagg acgttttccc ctgtcgacct ccgccggttt tgccgtgccc
    32041 ggaagagcat ctcgaaggtc aggtccaccc ccaggactct tccctgggga cccacccatc
    32101 tggctgcaag aatgctgtcg accccggcgc cgcaaccgat gtccaggacg aaagcccccg
    32161 tggagaggtt acccagggaa aagggattgc ccaccccgca gtagaagtcc gggacttttg
    32221 caggcaggcc gtccaggagt tgtgatggat atcccagggc ctgaaggcct gcaagcccgg
    32281 tgggataccg gaaagatcct ccggggtttt cggcaacccc tcggtatttc ctccggatac
    32341 tcgcgtgaat ctggtcccgc tgtcgggaag aaagacaatc ctccatctgt gtattccgct
    32401 tcccgtgagt tcatgaccgt ccgcagtagg tcggggattc cttatagcgc aatctcgtga
    32461 tccccgggaa gtccaaccgg aaagtgacgg ctggaagagc tcgaggtgga aaggcgagtg
    32521 aatcgggatg aggtcgggac cgaaacccgc gtcgggcatt ctccaacgca acgttgcggg
    32581 gcagcaggga gcgaaaacga aaggcccgtc tcaattggcg gccgaggagg tgagcaccag
    32641 tttcatttca taactgggga gctttggaga cccgttgcag acaaagcgga tgtcgacgtt
    32701 ggtttgcccg attcgccatt ggctggctct tcctgaacga atggaagctc gaacatccgg
    32761 gggcagaggg gtgccgatca tcttgtgaat gtacttgctg gcggagtcgg tgaagaattc
    32821 atcggccatc cggggttctt cgcaaaccat ggatacgttt agttttccta gcaggttttg
    32881 ttttctcatg ccctcttcct ttcgctgatg ggcgttccac tagtccaacg gacttcgaag
    32941 gactctcttg gccaccggtc gatgtcgtga agaggctatg gataactgga ttgtcacctg
    33001 ggagagatgg gatggggtcc ggtgagcagt caacggattt cagtgctaag agtagtatca
    33061 accggcaagg attgtcaata ggcgagttga agagaaaccg ccggaaggca acttcagcca
    33121 agacaaacgg cccggatcgg cttatatttt gccgtggggc cgaacccagg tctgaaactc
    33181 gaaggagggt cgcgctcctt tcccttgagg cgaaaaggca ccaagaccgg cacggacccg
    33241 ccccgtgatc ccggcggccc cttatctgtg ggtccgcttt gggaaaaatc ggaatcggca
    33301 gcagtgggaa cctggggccc tttcgggggg gttccacccg accctaatgg aagaagacga
    33361 agtcgtgggt tgcacaggtt cgacttgctg cgtaacttcc cgggccatgg agaaggacac
    33421 ctgcggtgcc ccgtccactg caaaagaagc ggagggcttc gccatgatca aggtagaccc
    33481 aacccgccgg gatgatccgg ctcatgggaa gctggcggag ggtttcggac tgaagggagt
    33541 ccccacggtg gggttcccgg accggaaaag ggaggagttc catggactga gggtcaagga
    33601 atccatggat ttccgggcct ttcctgcaca aacgaccagg gcagataggt tggacccgaa
    33661 aggagtgctt caatcccatg gcgttcaggt tttttgcaca aaacaagacc gcatccgtca
    33721 aggctttgct cttggtatta ttcctggctt ccgccctgta tgtctttcgg cacacggaag
    33781 tcgggagcta cctttctccc gagcgcctca ctcacctgat tcacacagcc gggatctggg
    33841 ctcccctggc ctacgttctc ctctacgggg cgttggtctg cctctttgtc ccggccaccc
    33901 tgatgacggc tttgggcgct gcgatcttcg gggcctactg gggattcctc tacgtatgga
    33961 tcggcgccat gatcggggcg tcgggggcct ttttcatcgg gcggtacctg gggcgggatt
    34021 ttgccgcatc cctggtcaac gaccgcctga ggaagtacga cgaagccatc gagcgaaacg
    34081 gtttcgccac ggttctctat cttcgtctga tctattttcc ttttacgccc atgagcttcg
    34141 ggatggggct gacccgggtg agcttccgag attacttctc gggaacggcc ctgggaatcc
    34201 tggtgggaac cttcatcttc acctttttca ttggtacggc caaagatgcc tggttatccg
    34261 gacagtggga tcggctggcg gacccgaagc tgttcctgtc cctggggctt ttcgtctttt
    34321 ccttctttat cccccggctg gtccaaaggc tcaaagtctg gagaaagctg cacctcgagt
    34381 gaggcccctc ctaccagctc cccccgcctc cgcctccgaa tccgcctccc gaagagcctc
    34441 ctccgccgag accgcccccg ccgactcctt ctccccgggg ggcggaaaac acggcggaac
    34501 ccaggctgcc ggtcatggaa ttgatggatc gatggaagtc tcgacggtgg gagaagtcga
    34561 tgccccggga ggattcatac catcgggcgg gttcctggta gaccccctcg aaggcttcgg
    34621 accagttgtc cgccacatcc agggcgatgg cgtagggaag gaacctcgag tacaaatcct
    34681 gctgccccat acgttcgatg cggtccttct cggctcgatt caggaactcg cggaacccca
    34741 ggatttccat ggcggcggcc gcccccgatc gggttttggc gggcatgacc cgcgaaaacc
    34801 ctagaaccac cccccctgcc aggatccacg ccacaatact cttccaaaag aaatcactgg
    34861 tgaaaaagta ggtgacggcc ccgccgacac cggccatgac aaaggcccaa atgacgtagg
    34921 atgtgcgcac ctgatccgga ctcttctgaa aacaccccat gcgtaccagg tcctcataga
    34981 gaacctgtct taatgccttg agatgcttat agaaattgtt cttgaggccg gacaccgaga
    35041 ttgtggtgga agaaaaagga aagaggtccg acatgagctc tttctcgaat cggcccaggg
    35101 cgtcgtcggg ttccttcagt ttcagcagct tgtagtccgt ggtatcgaag atcagcccct
    35161 cccggacgac ctcctggatt ttcatatacc cctttaccgc cagcccgacg atggtcgagg
    35221 tgatgtcccg gggatccagc cgctcgtcca ccaggcagcc cacctccccc gggcagaggg
    35281 tcctccctcc cagctggggt ggattgtatt gcacggtgac ggcctccttg acccgggggt
    35341 cccttcccac cgcgtaccag cgacgaaaca tcgccagaaa gaccgccggg ggaagcagga
    35401 acacccaatt ctccctccag ttgaatctcc accaaaaccg cttccaggcc gaaggttccg
    35461 ccaccagccc cttctcccag cccagtcgga cggtgagtcc ctctccgatc ttgaggcccc
    35521 cggcagattg aaaggccgcg ccgtttccct gtttttccgc ccgacaatcg gttgtggcgg
    35581 accccatgac gcccgtaaag caggccgacc acaggtgttg ggccggaagg tccccaggca
    35641 gggagaccgt tgccgaaaca cgggcgatgg aagttttcca gaaatttccc gtaacgttcc
    35701 agtacagctc gtcgtgatcg tccaagaaca agagtgcgtt ctccacctcg tagtcgattt
    35761 tgtacacctg gacccctgcg acataggcct gaggatcgcc gattcgaata tggacgacgt
    35821 ttccccttcg actcacccgg tagtcccaag gctgcccctg tgcattggtg accgagatca
    35881 cgcggatggg ggtctcgagg gtcttgccgt accggtccga gtatcggaaa gggatttcgc
    35941 ggaagattcc gtgcctctgg gcactgaagg tcacctggat ggtttcctga acgagcaggg
    36001 aggaatcccg gcgaacggcc acatgggact ggaactcgtc gatgacgaag tattcggcgg
    36061 cggcaccggg aggaaaaacg gcgccgaaga agataaggag gaagacccaa aggagacggt
    36121 tcttgaattt ctttgcaggg cgttcggctt ccatgacgat tctccccagt gcgggtgaac
    36181 acgggtccct tccggcttcc gttcctttcc cttgtggaaa acgcccggcg gggactgttt
    36241 tcccgggtgg ccggagagag gggcttccag acagcaccca attgaagggc ccgggcccct
    36301 cgacggaccg gaaggcggga ggaagggacc cgacacccga cggggttgca ggcagcgccc
    36361 gcgggatgcc tcgaagcccc ttgccctgcg gccccggcgc ccgtcccaaa ggtatcagga
    36421 gaagctgatc ttcacggggg ccctttcttc agcggcagcc tcgagctcga agaactgggc
    36481 cttttcgaac cgaaaccagg cggcgatcag gttcgacggg aaggattcga tcagggtatt
    36541 gaagtcccga accacggcgt tgtagtagcg acgggccgac tcgataccgt cctcgaggtc
    36601 cttgagatga gtctggagtt gtagaaaatt ggtgttggcc ttgagatccg ggtacgcctc
    36661 ggcgatggcg aataggccct tgagggcgct ctggaacgat ccttccgcct gggcccgttc
    36721 ttgaggagaa gcggcctgca tggcttgagt gcgggccttg gtcacgttct cgaacaccga
    36781 ccgttcgtgg gccgcgtatc ccttcacggt ttccaccagg ttgccgacca ggtcgtggcg
    36841 tttctttagc tgaacgtcga tgtcggacca ggaggattgc acggtgttcc tgagccggat
    36901 gagcttgttg tagagaaaga tggctcccag caaaatgagg accaggataa tgaggatggc
    36961 cgccgtcgtc atcgttgcgc ctccggtgtt ctgggcggag atcccctctc actgctgtcg
    37021 gggatggtcc gtgaagaaag ctgtagggtc tttcctccat caatggtaat gtccaactat
    37081 cggcgaaagg gcggaaaaac gcaaaggctt ttcccggctg gagggatgga tgcagggact
    37141 gttgatccgt tggttgatcc tgacactggc catcgtgctg tcgtcctatc tcatcggggg
    37201 cattcgggta agcggtttcc tcccggcctt ctttgcggcg gcgctcctgg ggctcctgaa
    37261 cacattcttc cgcccgatcc tgatcttgct tacccttccc atcaacctta tcactttcgg
    37321 cctttttacc ctggtgatca atgccctgat gctcaagatg gcctctgggg tcgtccccgg
    37381 atttcaggtc cagggattct gggcggcgtt ctggggcgcc ctggtgatca gtatcgtaaa
    37441 ctggctcctg atttccctgt ttcgagaccc ggtgatcttc gactgagaac acgccccgcc
    37501 cgtattacgg ggtttcgtcc catctacgga ggcgagcgcc ggcagacctt cctcggcctg
    37561 ggtccccttc ccccctcacg gccccttgta gacaaaagcc ctgcggcact cgtcataggg
    37621 gctgagcttc ccatccgact cccgttgggg gggagttcga aggctgggtt cgagaggccg
    37681 ggcgctgcgg tctccgcaaa gaaggaaata aggcacggaa cggtcagcgg tccaaaggaa
    37741 aggggatgtc gtatgagtcg tagaagagcc gtggtagtcc ttttggtggt tgtcttgacg
    37801 ggttctctgg cgttggaagc cctggcggcg gaaaggatgg ctttccgcca ggattccatt
    37861 cgaaaactga cctcggggag cagggtttgg aagaacgtgg catccgtccc cttcacccct
    37921 cccgaggacg ggtatgtggt ggtgacggcg agcggcatgg ttgggttcga cgcggaagtg
    37981 tccagcctca ccctgtccct ggacaccaaa ccggccgtca ggggaccatg ggttttcacc
    38041 ctgactcccg ggtaccagct ttttcagacc tacagtgttc ggtacgtctt tcccgtgaag
    38101 accggcgtta agaatacatt ctatctgaac ggcctgtcct gggacggttt cgggaggtcc
    38161 atcagcattg agaacggctc cataaccgtt gagttttacg cctccggcaa cgtgagggcc
    38221 ttcggtgtgg aggcgatgaa gtcggtgcag gccgcacccg gcgacgagcg cggcaacgaa
    38281 taagagcccg gcgtggtgca aatcctcaag ggccggtccg taaacaagaa gtcccttagc
    38341 ttcacaacct tctcccaccc gggggagggg gcgtttcccc ctccctcgat gctttccccc
    38401 ttccccgagg gagaggggtc ggagcaatgc ggatgacctg agttccctct cccttccacg
    38461 gggagaaggc aatcccccta ccggttattc ctcctttcaa accccgaaaa ttctcttcaa
    38521 gttccgatcc caagctgccg accctaggaa caagatgcgg ttcggcccct tgcagaaggg
    38581 gcaagaagtt ttgtgagcaa tccaggcaaa ccaaaggacc gtggacggtc caactgactt
    38641 ccgtttggaa gaatcagggt acccagctct cgatgagccg tccgggcaac cctcccgtgg
    38701 ggtcaacccc aggggagggc cccccggttt tggatgggtg agctcaacgg ttttctctct
    38761 cgtgcgtcga tccgcgggcc gaagggtgaa atcgggtgct tcaccaaagg gaaatcctcg
    38821 tcgaaggcct gcaccccgtg ccgagaggtt ccgggccgaa acgcacgggg acggcaaacg
    38881 gggcccatgc cctcgggatg aaaggaaacg gggtcctttc tccgtctgaa tcggaggatc
    38941 ccgcccgggg aagttccatc gtgtggaccc ggatgtcccg gggtgggccc ggggatcgaa
    39001 cagccaaagg atactcgcgg tgattggggg aactgtgatg tccgtccttt ggaacgggac
    39061 caacgatctg ccgggactgc agcagatcgc cggtgccttc gccgagttgc tgggcaacgg
    39121 ggtcctgttc aaatacagca ttgccagcct ctttcgggtg acggcgggat tctacctggc
    39181 cgtcctcctg gcggtccctt tggggttgtt cctggggcgc cgtcccagca ttcagcgtct
    39241 ggggaacccc ctgatccaat ttctgcgtcc cgtgtccccc ctcgcatggg tccctttcgc
    39301 cctgctctgg ttcgggatcg gcgaccagcc ggcggtgttc atcatattcg tcgcctgttt
    39361 cttccccatc ctgctgtcca ccatcgaggc atcggccgga atccggccca tctattttca
    39421 catagcggca aatctccagc tcagccggtg ggaaaccttg agctgcctaa ttttgcctgc
    39481 ggccgctccc agtatcgtcc tggctttgcg ggtgactctg gggatcgcct ggatggtggt
    39541 ggtggctgcg gagatgatcg cggtgaagag cggcttggga tacctcatcg tggatgcccg
    39601 gaacgccctt cgcatggatt atgtggtggt ggcaatcctg accattggag ccatcgggct
    39661 gcttttggac ggggtcatga gacgattggc gcagatcgaa tccgtgaggt ggagagttgg
    39721 gcaataacaa aatccgcctt gagggggtga gtgtggaata cggcacggga aaaggctgca
    39781 ggcgcagttg gttcggccgg ggaaaatccg ccgggggaac cgttgctcct tcgacccggc
    39841 tggtcctgga cgacatcagc cttgaaatcg aacgggggga gtttgtctcg atcctcggcc
    39901 actcgggatg cggcaagtcc accctgctca aggtcgttgc gggttttacc cagcctgttc
    39961 gcggccgggt cttgatcgac gatcagaagg tcaaaggtcc ccggtcggac catgtgttcg
    40021 tgttccagga aggggccttg ttcccatggt tgacgatcct ggaaaacgtt tccctggccc
    40081 tccggtgggt caaggacagt gtcgagaaaa agaacaaagc ccgagaatac ctcgacctgg
    40141 tgggcctgga agggacggcc gactattatc cccacatgct gtcggggggg atgaggcagc
    40201 gggcggaaat cgctcgagcc ctggctgccc gacccgacat cctgctcatg gatgagccct
    40261 tttccggtct ggattacctc acccgcctcc acctccggga agagatgctc tacctgcagg
    40321 tcatgctgcc ggagacgacc attctgttcg tcacccacga cattgacgag gcattgcagc
    40381 tgagcaaacg gctcatcatc ctcagcgaac ccccggcccg aatcaagtgc tgtcgaacca
    40441 tcgaggcgcc tcatccccga agcctgagca gtccgatgtt gagcaccctt cgaacccaga
    40501 tctatcacca cctgggagtg catgcagccc tatgaaggta ctgtcctttc gaggttcctc
    40561 cgcgtccttc aaagccttca tcctggccct gttgtggctg ggggtgatct ccctgctgca
    40621 cgcctcggtc aaccgggaga aaacggcttc ccagaaggtg cgcatggggt acatgcccgt
    40681 catcagcaac ctggcggctc ccctggtgga tgccgccact gccggttccc cggtgcattt
    40741 cgaggccctc aaattcggct cgtttgcgga aattgcggaa gcgttccggt cgggacatat
    40801 ccaggccgcc ttcatcatcg cacccctggc ggtggcactg tcttcccagg gggtgcccct
    40861 caagattgtc tacatcggca accgccatga aagcaccttg gtggttcgaa aagacgaatc
    40921 cgccgcctcc ctggcggaaa tgagcgggaa gactctggcc gtgcccatcc gctattcggg
    40981 acacctgctg gccgtgaaac gctacctgcg tcaccacggc atggaccctc gggccgttcg
    41041 aatcctcgag atcccgccgc ccgacatgcc ttcggcccta gtgacggggg acatccacgg
    41101 atacttcgtc ggggagcctt tcgcgagcag gtccatccag tcgggtgtag gccgcaggct
    41161 cctcgatgcc gaggagatct ggccgggctt cgtctgcaac gtgctgatcg tgcgggacga
    41221 gctgatccgg tcccatccgg agcgggtcgg cgacctggtg aagggctgcg ttcggtcagg
    41281 aatccgggtt cgtgaaaatt tcgaggagac cctggggatc ctgtctcgct actggggaca
    41341 ggacccacag ttcgttcggt acgtgttcga gaaccctccg aaccggtttc gctacgatct
    41401 ctgcgtgccg gtgcgcagtg agatcgagga actggttcga gagatggaaa ccgaaggcct
    41461 gatctctgcc tcctcggagg tcggcgactt cctggaagac cgctatgccc gggcggccgt
    41521 cgaaggtctt cgagagccgc tgctgggatt gacgaaaagg tagattggat cgccccggga
    41581 aggctcggaa cgttcgacct cccgtgcggt cgcgcttggc aggagaagaa gaggggatga
    41641 acatcctgat cgtgggtctc gacgcacaga taccggtcat agaaaagctt tcggcagtca
    41701 tggaagacct ttccgtcttg gacggcctgg agacggactg ccggcccggc gggtcttcgg
    41761 aggtggatgc tctcatcggg tccgtgtccg agaaggcggg tcccggcaac ttggacctgc
    41821 tggtgagctg gcgaacccat ccccacacgt acctggtgcc ctgctggatc gcgggcccgg
    41881 ccccgactcg ggagacgggc tgtctctggc ccaagctgac catcgatcga ttcgaggaaa
    41941 ccgtcgacct ccccggattg cagcattggc tcgaggagat ctcgcactgg cagcagaacc
    42001 gcatgctgct ggctccgttc aacacctttg acaaatgcag ccccctcgag atcgcaagtt
    42061 ccctggctct tcgaaaagcc acggggcggc ttttgacctt cgacgacgaa aaccacgaag
    42121 gcaccctgtt cctgcgggag ggccgggtaa cgggggccgg ggtgaaacat ctgcggggag
    42181 aggaggcttt ccacgagttt ctgtcgtggt ccgaaggaag ttacagctgg gacccggaaa
    42241 ggatagggga ggacaacatg gagccgcgac tcctggagga gctcatccgg gaggggctgg
    42301 ccctgtttcg ggaggcaaac ctgttttatc ccctcgttcg ggactggggg cgttgcctgc
    42361 ggcccacggg gtcccaatcg gccctggacg acgccgccgt tcccatctac ccggcccagc
    42421 gggagctcta taagctcatc gacggtcgca ccaccgcggg gggaatcatc gaagcgtcgc
    42481 ccctctcgcg gcccaggact ctcagttgcc tggccaaatg gctgtccatg ggggatatcg
    42541 aagaagagcc ccgggatgcg catccctcga tctgccgg
//
LOCUS       DAOOVC010000018        40471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_629_length_40471_cov_5.995249, whole genome shotgun sequence.
ACCESSION   DAOOVC010000018 DAOOVC010000000
VERSION     DAOOVC010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 40471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40471
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_40471_cov_5.995249"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="PLM79_04245"
     CDS             complement(<1..365)
                     /locus_tag="PLM79_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV85548.1"
                     /translation="MLRVNDLQVYYGGIHALRGISLRAPEGRIVTLIGANGAGKTTTL
                     RAVTGLVKPRSGSVRLGGEELTRLDPFRIIRKGIGVSPEGRRVFPNLTVLENLELGAY
                     YRTSREISAELERVYSLFPR"
     gene            complement(349..1131)
                     /locus_tag="PLM79_04250"
     CDS             complement(349..1131)
                     /locus_tag="PLM79_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85549.1"
                     /translation="MNVFLSVEHLFVQFGGLTAVNDFSMHLVPGELVGLIGPNGAGKT
                     TVFNVIAGMVKPTSGRVVWRDEVLDGRPSHEIASLGIARTFQNIRLYRDMTVLENVMV
                     SFHHHMRSPLWQVMLGLGTGVRQERLIREQSREILQEFGLAHWALEKAGVLAYGQQRR
                     LEIARAVAARPQLLLLDEPAAGMNPRETRELGDLVQEVRRKYSLTVLLIEHDMRFVMN
                     LCERVKVLDHGFTIAEGSPSEIRRNRKVIRAYLGNSADAPGE"
     gene            complement(1142..2182)
                     /locus_tag="PLM79_04255"
     CDS             complement(1142..2182)
                     /locus_tag="PLM79_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85550.1"
                     /translation="MKPTGRRTNLLLNLASLLLLAGTLFLFHRYGSDYQVRILNNMAI
                     FIILAVSYNLVNGVCGMLHLGPNAFITIGAYTSALLTMSPAEKELSFILEPLVWPLSA
                     IQAPFSVSLLAAGLLAMGFAFLISFPVFRVRGDYLAIVTLGFGEVVRVLCNAMQSVTN
                     GPLGLKGLVPHTHLGWSWGVAVVTVIFVTRLTNSSYGRAMKAIREDEIAARAMGVDPF
                     KHLLLAFMVSAFFSGVAGGLLAHLITTISPNLFTFLLTFNLLIMIVVGGLGSTTGAVL
                     GAALFTWAGEAMRVVERPMNFGSWSIPGIPGMRMVIFSLLLMGVILFRRHGIMGRREF
                     SWDWLRGRRGPS"
     gene            complement(2192..3085)
                     /locus_tag="PLM79_04260"
     CDS             complement(2192..3085)
                     /locus_tag="PLM79_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85551.1"
                     /translation="MSATVFFQQMINGISLGSLYALVAIGYTMVYGILRLINFAHGDL
                     LMFAAYSALYGTTLFALPWYWSFPLALLGTGLLGILVERSAYRPLRGAPRISLLISAI
                     GVSFLLENLALVVIGGVPKPFPRPEVFDRVVEFMGIRIPVLTLYTPIITLVLLSGLLA
                     VVFRTRVGKAMRAASTDFETTQLMGINLDRIISVTFLLGSVLAAAGGIMWSMKYPQVN
                     PFMGVLPGLKAFIAAVLGGIGNIFGAVLGGFVLGVGEIFIVALVPQLSQYRDALSFLA
                     LILMLLFRPTGILGEPVEEKV"
     gene            complement(3115..4236)
                     /locus_tag="PLM79_04265"
     CDS             complement(3115..4236)
                     /locus_tag="PLM79_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV85552.1"
                     /translation="MHAMVVFAVCFCLTAGSVRAEDCRDSLRIGVYLPMTGSVAAYGD
                     MEWAGIRIARDMEPEALGRKVELLLVDTKSDKIEAANAVTLLIERDKVVGILGEAISG
                     NTMAGNPISELAGIPSVSPSATSPLVNQGKKFAFRACFIDPFQGQVAARFARSRLQAA
                     TAALIIDNAQDYSVGLGSFFAKQFTKDGGKIVSTAYIQTGDQDFSAQLSTLQAANPDI
                     IYAPNYYTESALLAKQARDLGIRAPILTGDGAHSDTLIRIGGNAVEGMFLTGHFHRDA
                     AGSPRAKEFIRRFTEQYKRDPDAFAALGGDAYFLLIDAVRRAGSTDGSRIRDALASTR
                     GLDAVSGSISMGEGGTPVKGMVINKVENGGFVYVTTIEP"
     gene            4872..5129
                     /gene="ffs"
                     /locus_tag="PLM79_04270"
     ncRNA           4872..5129
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLM79_04270"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            5339..6025
                     /locus_tag="PLM79_04275"
     CDS             5339..6025
                     /locus_tag="PLM79_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85553.1"
                     /translation="MPGTTVLVVEDDPDILNLVSYNLRIAGFSVLSAPEGHEALGLAK
                     QHLPDLIVLDLMIPGLDGFEVCKELKKTSATRNIPIIMLTARGEEIDRIVGLELGADD
                     YMVKPFSPRELILRIRAVLRRTGSDRRDETLLQKEGIQVSLESHKVWVEGNEVILTAT
                     EFKLLVELLRAPGKVHTRDQLLDKVWGYHFEGYARTVDTHVRRLRQKLGVYSEAVETV
                     RGVGYRFRDA"
     gene            6030..7865
                     /locus_tag="PLM79_04280"
     CDS             6030..7865
                     /locus_tag="PLM79_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85554.1"
                     /translation="MSGRKSLKNSILYYCWALVLAGVLPCGYLYHLLSRTAVEDAQGM
                     ALRELDLVERLLTDAAPFPKVEDLQDRVTRAAKQRGVRISYVAEDGRVLADSMTDPDR
                     ISDLENFAGRAEILDARSGAPGVSFRYSQAVKKEMAFAAKKTALLEGHPPGFLRLAMD
                     PPSIQGQLDRFIQAVPLYLFLLCAVVALSVPLVVRRFDGILSEMTRAVDSLGSGRPAR
                     RVHLEPGHELSPLALSLQQCAERLEDRLASTNEERRRLEAVFEGMREGVIVLDSRGRI
                     RSVNRALANLVPRTPSPVGRKPIEVIMNLELQEACHAALSPDRPSNESFRRLELVLWR
                     ERSYTVTISRLPPTRDEGEDEIPGAIVVFHDITEIKRLEKVRQDFVANVSHELRTPLT
                     SVKGYTETLLSEEMPDRQTLASFLQVILKNTNHMVKIVDDLLELARLEALQFPVRPVP
                     VHAGTALHAAWKACLPLASAREISLQNDLPPHGPWVLAVPDQLVQVFRNLIENAVRYN
                     PASKPIVVSCEEQEETVTFRVRDFGPGIPKEHQDRIFERFYRVEKHRAGEAGSTGLGL
                     AICRHILRNLGGRVWVCDPSPGRQEGAAFCFTLLRAPAEANRREG"
     gene            8220..8981
                     /gene="pstB"
                     /locus_tag="PLM79_04285"
     CDS             8220..8981
                     /gene="pstB"
                     /locus_tag="PLM79_04285"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOV85555.1"
                     /translation="MEKSPKMASESLNFYYGAFQALENISLEFYPQQVTALIGPSGCG
                     KSTFLRCLNRMNDLIATSRVEGRVLLDQEDIYDPKLDVVTLRRRVGMVFQKPNPFPKT
                     IFENVAYGLRVNGVRDRSFLAERVEFSLRRAALWDEVKDRLHHSALGLSGGQQQRLCI
                     ARALAVEPEVLLMDEPASALDPIATQKIEELIHELKGSYTIVIVTHNMQQAARVSDCT
                     AFFYMGKLVEVNETEVLFTRPQYRQTEDYITGRFG"
     gene            8993..9652
                     /gene="phoU"
                     /locus_tag="PLM79_04290"
     CDS             8993..9652
                     /gene="phoU"
                     /locus_tag="PLM79_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOV85556.1"
                     /translation="MENRFHQEMEELKMTILHMAVLTERALENAVRSLVDRDGDLAQA
                     VIDGDREINLREMEVDRRCLRLLALDQPMARDLRFIVGCLRISIELERIADQAVNIAQ
                     RAQFLGSRPPLPRNPAIEQLADTALDMYRSVMSAFVNENTNLARDVCQTDDLADELNV
                     TVLKNLLDYMVHEVPAVERSVQTIIVARCLERVADQTTNIAESVIFIVEGVNIKHRCQ
                     Q"
     gene            complement(9672..10565)
                     /gene="pstA"
                     /locus_tag="PLM79_04295"
     CDS             complement(9672..10565)
                     /gene="pstA"
                     /locus_tag="PLM79_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOV85557.1"
                     /translation="MGRGSGPGPARPVSGTGFRRRLQVVVFTLFRGAAVVNGLVLLVI
                     LGFLAWNGWRSVSWTFLTQAPTNSMTAGGIFPCIAGTFLLSFGAMVVAFPWGVASAVY
                     LHEYARPGMPVRMIRLGVTNLAGVPSVVFGLFGLALFVTWMGLGVSVLAGVLTLGALV
                     LPVIIGTAEEALKAVPATYREASLALGATKWQTIYRVVLPAAFPGMLTGAILAVSRAA
                     GETAAIMFTAAVFFTPHLPSSLLDPVMALPYHIYVLATAGTNIEATRPLQYGTALTLI
                     LMVFGMNLVAIYYRARLQRRF"
     gene            complement(10661..11554)
                     /gene="pstC"
                     /locus_tag="PLM79_04300"
     CDS             complement(10661..11554)
                     /gene="pstC"
                     /locus_tag="PLM79_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOV85558.1"
                     /translation="MMTHRQIKETFIRWFFFATAFVSILVLFLILVFLFMEGVPIFSK
                     VSVWKFLFGTNWYPTASPPDFGIFPLLVASVAVTTFSALISIPLGVMTAIYLAEIASA
                     RMREIAKPVVELLAALPSVVIGFFGMVVMAPFLQDYLEIPTGLNLFNASLMLAFMSVP
                     TICSISEDAIYSVPRELKEASLALGATHWETIYRVIFPASLSGISTGVILGMSRAVGE
                     TMVVLMVAGGAALVPTSIFKPVRPMPASIAAEMAEAPFRSDHYYALFATGIVLFLFTV
                     LFNLLADMVAQKHKQVGAATL"
     gene            complement(11736..12560)
                     /locus_tag="PLM79_04305"
     CDS             complement(11736..12560)
                     /locus_tag="PLM79_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOV85559.1"
                     /translation="MRSKGKGRVFLIALLCGIFAATLSHAGSVRIDGSTTVLPITQKV
                     MEAYMKENPGVSISLSGGGSGNGIKALIDGTTDIANSSRFIKDEEVKAAVAKGAYPVP
                     FALAYDCIVPVVHPENPVKNLTLDQLKAVYMGEIKNWKDVGGQDKEIVVISRDTSSGT
                     YEVWEEKVMKKARVYAGAMLQASSGAIVQAVAKNKFAIGYLGIGYLNPSVKALTVNGI
                     EGNEETTLTGKYPVSRPLFMFTRGWPSGDVLHFVNYVLDPHKGQKLVKEAGFVPLY"
     gene            13445..19987
                     /locus_tag="PLM79_04310"
     CDS             13445..19987
                     /locus_tag="PLM79_04310"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85560.1"
                     /translation="MAGTNRPRLSRRTRGAEKGLPSTAHSPGDWALVENSSPAFLAVR
                     IGGDLCRVIDRESLGKDLCLLLIGARYAQTCRERAWPPPLDGLLRQEAHRSRFHILCL
                     REDAQPGKLLPVGTLMEDSTGDRHVIEVSPHATGLLGPILLAAAAELSCRAQDPSRYD
                     SFGDFLYPGGPNLVERHSTERLQKHLGRKVVYDALLCNHCLRCATVCGEMKAVTARGN
                     LQLLGPGEHFCTACGLCQMRCSYLATIPKEGSSRSADPRLRLHEGGAALHLYGDTAFR
                     FRDLLRRTVSSGDSIDFPYRVSRLWEIPPDGEIPPEIPLQFKIEARDPEGGLPGQALL
                     ATFPGDGSGGETAVVRSCRTAGLVLQTEEGDVERDIAGQALALGLEVKLILDCERTGI
                     SVPRNPLTEWFHRLGQPALVGKSLLQAWESGEVDFFLAPYADEVSPELQRRILEQTGR
                     TAHILAPNLLQALPFSKSPLLWQLNRSLWKVYLRHPELLAKEARYASELLENLPANSA
                     VLHARYRPLAFASGHSACPSCAEAMVLSIPVYMAIAMSLARGEIPRVSFTCETGCMSE
                     TLNKVGEVSQKVPGGRTVFGGGFAFGEAIAMVQDHAVRRGVLPKGRRYVISQGGDGGA
                     VIGLPAWLNALRQQARLIRQRHPNVLHFINITDTQVYSNTGGESSASSLLGMGTLTTP
                     IGRFLLGNQRTQWSLINLAAEFPGILVGAGHSANKLGLQQFWHLADQLGESAIHWDIS
                     PCPETGKFYGEDPDDLAELMSHAGMIPEILFVGRFRKRVAPYHPEDRNKPYREWRLQP
                     KPVLYWLRRDPRYRMLFKTNPETGEMEVRNLTCHYLVTQIESYRDQVNRQIDLESRIV
                     RRAEERVSEFFQELRESWEHYRFRLGEFPYAMLFNDRGERKPEYGIGLEYEMVRRILG
                     WEEATGVLDRRDRLLNHQRSRLEELVSELESLDGGSVAQIVESDPLARRHGAVKDLLI
                     QIQKTEEILQEQARGLRAAVNGTLARDPVEDEIFGPPPDSVEALARDRMRDLYGLLDR
                     IIEERAVARQVELQQHRLARTLTRKFEEEGGVFGRPRAPAHAENGRELLDHIRRFGPF
                     SVGVASLAGDRGIAVNRVFAGFFSAKGAWAGMAWQFGSSKRGTPVLSATFVDARPFQR
                     KDAMQSVPVAVLAVTNFEELKRSPDLLFGGMRPGGYLIINHRKSARALWHELVAFFPE
                     EVRTWVAGAVEASSGKPISSPTFPGGNREEHGPPGLPGSGTTWTDLSEPLFGKPFSLL
                     TPGQKEWTRKVAAISTVRILSLDMDGIIQEVTGSRKIVSNLVAVAPLFHALQEQGFPF
                     RWEEDRDLLVRGFPEAVLKNPQLLAQYERGMELARERWNSWTPVPASPAPPSPAERRD
                     PSSLVSAKAEEVPREESPSDCLMIMGGTLAGMVLSQVALEEHPLFYIGFPITPAGNPF
                     YAMAEAYADGHPYIVVDEVNPSEKVAAEKLLGIARTGGFLPVTFTASQGWRLFTEILP
                     QFVGARLEGLFIITRRALAAPNLNIEESHTDFLSFRDDGGIMLSPKGMEEYVPTLYLA
                     RILTHFAKLPVILSIGGITDTHKIGLFKVPPDEEVRTWLRKTLADFDFLEDKLLNPQE
                     HLIVHGPSGTSSVYQETQSEIEKAHRAVQRIFPYALRAVSRLTGTALSEIEVAGTDPS
                     PQRTETLLILQGSLFPNAEAALLDLEREGWRGLACISVRCFNPFPEEELLPWLSGARR
                     IAVMDRSNSFGSLPPLASRVFSAVARHLSRPWADHPRQLRSLVGGLGGRMITVAQMRE
                     ILLSTHLLFHPPREWETGLVREWIASDETLRALLEEAAALDLRNTDRHSRVPESLRPA
                     SARRREYEERLAALSRQLLEKDYRSLLANYHQVEFIEPREVLLETTLLQQIVQYVETR
                     LARHALDNGLASPRHAQLLNAYSTSEEDLERARVYLEGAAEAREGFPGAVSVSGDSEI
                     HSSPPAEELKETEPGDPIPDPRSDRSGPLPAGEPDEPADFAPDPREAELIEVHLTSLA
                     ALQGELPLYYNPEDFEHEVLRLLQKDPGSCLFELSRKVPADRLPRLLRRYRSCYADVI
                     DRVLQREVLSMHHAPELRGLFEGEGKRRLEALALRILPYGEGMDPERLERWLTGELER
                     YLAEVLLPGCPKAPIFYLEYFRQWIAPDLVKTALDPLHAARTKEDFQA"
     gene            20245..23154
                     /locus_tag="PLM79_04315"
     CDS             20245..23154
                     /locus_tag="PLM79_04315"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV85561.1"
                     /translation="MTNEPLIVSFEKADPTQVDRLGAKGAKLVEDFRHVPEMFPGMED
                     TISVPDGFIISTDAWRLFHKGGDRLNDELFARILLDLSSLERRVGKQYGACTGRMPLA
                     VAVRGGAPISLPGALGTVLNVGLNDELVAHLIEAGMDESFVLTTYLTAIRMYGEVVLD
                     IPYEPFYAALRRYHVGDEGTIPVPVLHSLLIEFKGILDRHSHPRFPSGFEQRPDYQLR
                     YCIEGVLASWMSPTAVEARRSRRGQTGGISDDMGTAVIIQEMVFGNRNDHSCLSGVLF
                     TRDPRTGSDNFLIEWAPKVQCDKIVSGKLRKQLLHADDLQKRFPDVHRLLSLARDRFE
                     TWARRPLDIEFTVEDNHLFLLQRRPLRMTSNATVRAMWDLVDEGKTTIQLASLIINKA
                     LEQPEKVLKSGFHDYVVLAVGEPITDSADSGILTVGTEAALELAQQGREVILLRRNPY
                     GESDVAVNHPNVRGVIRCDGNATGHEAVSAVAYSKPYLINMADASGCPLICGDGEERM
                     LNPDSLLARYVGKRVFVDGEHGILGYTEHTDFLEDRKSRKRLYVDWEYLQERFDDGRY
                     DQLSYEELLDLHYHWELELDQYLQLEKSLRDGDPSIPTEALLQAFTLYLACIPEKKRD
                     RVLHLKGVKVEDFVFEPSVRYLGNHLGQEVRKILRALMLCTTWRTHWLHEIMVRLARE
                     RGETENDVIRDIFLKNQTMSLVRDFEEEGFHLMKVSRYYHLILASNFEYGQDLDKVLV
                     GPGAMNFAEKEFLARHFLDFLNEVNPELRSRVRMVSGEPPLGQGHARIISIGLAIVHE
                     DFGLVCRYLRSFLDQRKQGMLARLPTRIPVSEFVELYHLDPFFAPFPDLRISREASNQ
                     LPTGDGLLTFGSCSFGEYEGSFYGKEDYDRLGAEVRRFEQYLRKSGRTLEVRPWQFEV
                     DPYRRHSVIAAVGIRFDLEELDVLMESLKGFLVPSTEKPAPGTLEVQGLRPENRV"
     gene            23157..28154
                     /locus_tag="PLM79_04320"
     CDS             23157..28154
                     /locus_tag="PLM79_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85562.1"
                     /translation="MLTEEMRRTIYSELEAANPAAGKPSSPKHGKVPALPGPGADLRA
                     QWAQEVHARTEIVLDRLLFQLHSLFDFREDESRIQAVVRRALSEVYDIENSCRGDGSY
                     DAFSYALCLFRKRWHEGSRGVGDSGLSGLAHVDRAQFFSKVPQEIHTWARRFLLVERY
                     HRLHPREGENRLDRFRLLRSPGPYSEITSLDDFLVRLDFRFMEESGREDPDVGSMARR
                     LAAEIQGRLSPDLFPGEPLQPLVYLFLRCHRECLSEVVEPEEKRRLDHLHSTRQMDSL
                     LDLMRVVIHRTTREHRLPEAAGILDSLKEEGFNISHAAFRDPYEADLVPPADTERFEQ
                     SGLPLFLPLDSLTGDFSADLEWSRKPIEEWNQRIVREVSLEVEEYCRASSQPLPRARL
                     VLKLIRFAVSRALARTPWNEIEWKEDLPTKVLEWIPGPVLRSLIRPRKGYEIKSKQEL
                     VQRISMECRLQCEALERIARTRRIEGYHDYRYFGRYVLFQGIQTSSSAPLGPEVRELR
                     HRLPHLPDPRWLECAGNSYGLDISSVHPHTLDCVPRYLEGRVPLSMLVSELEKNFETS
                     ENELYRVARHTLRSLTPSFHRLLVAFARRRARLPRPECSPRDIPGLIQLLAPPLLSLE
                     DDARLHILGHLRQHGGGLYRDQHPMCLFTEAEWKAALAWEVKKRIGDSPRHFLENGNP
                     DQLLHSIEESWAGMQVPLGPLTHQTVQGQLIELFRPADSALSVPDAALGELTRLLPRK
                     DCGACGAPGCREFVQHLVQGLGEARRCPHLSPKQLSEVVRRAAELDKSSSQDGRQNPN
                     LLELLYHRSLWRGSEEKRTFQQVLSPKVQQSRRRFLERLEDLWNNLSPKPRIFRDLDE
                     ERIYRDLCLYLGYEAVERLGEEERDLLVQGGEEKRRAEWRQLKERRNWLALADEKRLS
                     RPLLAARDVHRTSRETYDSVLFLHQLHPDDRRLVLRHRVNTFEDGFSRWWNEDLLTMN
                     LPGYSIRDWEDFSKIIHNAYWHQESSVLPGEALAELEKEVLTRPDLSLLVEKLVGRWI
                     DEEASRIARAADDLRSFRKGAERRPIGNVRDLRRIFEGLLNEAEALRTLATPSESSGQ
                     PSRPGGEDQRKRLAMTLWDRFQEEGFLFSPSFSFHWDELQPDERRAMENPAWGAGNGG
                     SASPAEGVPPEKGPDGARERVSVVHAVLSTAVNRRCREQREAAWVEERTASTPVPLGS
                     LLVYVGRRLRQGQGSRELHREIVDLLGSNSSLRERVLKDALFRFIAGHLRKSLENPAS
                     VLPVSSLREVGGDRLPDPWEDLEPSLSRILDGNPPMDRERLFRYLFVLAKMEGNLDTL
                     TALLREIRETSDIIEAAWLKFTEERMVEGPSPKAMPGTAPGIPLLASRLKDKGPLNRG
                     LREGVSRKEKKAVASAVRELENLLRFHILLATDHGGKLPDPEAVIGEMESAGYDLTGI
                     EEESLRTAVRKEIDRRERWAAHKIWIYTTAVARRLAAQHSGLQEVERELHKHRMDLLK
                     HEPGRGGEFHRMASLRGVALGRIKEELYRQLSDLLEAERIASFQKRIRQIVEQLDQKR
                     REIREGWVRGEINRRTVFYLLRQYQKGEEEPTWEDFLVFVRDHWLLPLERLRSSDRPD
                     REKRIRDLDARFQALLGVSLLALEAETSAQADRDLESWIQDRSASLQGTLSMGKG"
     gene            28158..31904
                     /locus_tag="PLM79_04325"
     CDS             28158..31904
                     /locus_tag="PLM79_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV85563.1"
                     /translation="MSLSSQPAAPFGFLTSNSPPGDWIEQHRQAHQHYRGPRDTRIPP
                     FLGGLAQAVSTLRFSLMQLRLALERTPSQPTDFRRRLLDSGMGLHGEDPEGHLVKLVD
                     NYLSRRDWREQDILEMILEYLERSSPREGELAGELARLRSLRERDRERYQRVVPGMMS
                     EARGNLMGLGYASRFLQDLKPRFPKLNRGALEVLTGALRMSLGLPRGAIEEIATRETP
                     ADLWSRFYKEAGAHWTAQGTFLTLKPFEKASLYLMESLPIEKRGHSGFVRYLLENEAA
                     YRRILAACYASPEEQQNRLLEETDRYNRLPEHALQPAAYDEEERALFLYAFFLRPDVV
                     EAIAERERHKDLYQAVPGLPSRALLPRVLQEVQNVFGYITPESFEKIVEILELPPVEV
                     IRVIASYKQYSADPGGDILIYVCKGTACFLRGQPNLSRSLSSHIRAEQGEVGEHGIQF
                     IEMDCFGVCHLAPVIKSRDTFLGNRTARDIPRMLDQLLKGPEYDNRLSFIRRIQQELA
                     PGRTSDLPDTMRILRVEGNDGAKSPSPGTVGEGPIPSEKDLGGAGLRIDSDGRVHAVA
                     AEGSRELGRVIPSCLPFHYTGRDGSVQVGCVVLDEFGQATEFIHVDADFLGDDLRRTM
                     KPAVRVTEGKVWIERNGPFLCLGDYIDQAVAVRSETGAARLVVLSGTPGRTPPAREEG
                     VARGSADGEDAGFAARQDRLVLGFAALADPQDIDSYLAVGGYGAVRRVLGLEGEPAWA
                     PRDIVREVSAARLRGRGGAGFPTGTKWEAVRLARCVVEEGDHNRDPIKFIVANGDEGD
                     PGAFMDRTLIQERPHQVLEGMILAALAVGARYGVLYVRKEYEDAVRRLEHAVFQARRK
                     GFLGRNIFGTPGLCFDIDIRLGAGAFVAGEKRAIMRAIEGEPAEPTLNAVSNTFRGLW
                     GKPTLLNNVETLANVPLILRKGSEWYASRGTENSGGSKIFSVAGIVNRTGLVEVRFGR
                     TLDDIIRICGGVQDGKRLAGVQIGGPSGAILSLTGVRSYLLYTPLDFDTFDRVGAMLG
                     SGGLVFIGDDDDVVRLARHFTDWLAEESCGQCPSCLQGTASLGSTLDSILAGEGTADH
                     IHALWAKSDAIKAGSQCGLGMTAANPVTSALRFFPHSFLYHALANPRLNRQECFLLLE
                     ALRLLTREDVQRITGRRRETLGYSFTLKKHLARHLMTELDRLDQVHPGEPRRVRQLLD
                     LLQVPPHEMGTRDISHECSLEELEDHRRYLEDWAGDPEPPSASR"
     gene            31954..32523
                     /locus_tag="PLM79_04330"
     CDS             31954..32523
                     /locus_tag="PLM79_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOV85564.1"
                     /translation="MNSSQKRFFDSFREVVKAVSSTLDLNQVMDLLVKNVTEAMELKA
                     CAVRLLNPKSRTLDLLASCGLSQDYIRKGPVDADRSIASAAEGNIVVVRDAGKDPRAQ
                     YQQEAVAEGISTIVSVPLSVKGRTIGVLRIYTHQPRDFSQEELDFAQALAEIGAIAIE
                     NARMYERIKKDYESVMSDLYTFVGYRRSI"
     gene            32546..32947
                     /locus_tag="PLM79_04335"
     CDS             32546..32947
                     /locus_tag="PLM79_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOV85565.1"
                     /translation="MSNRRVRILMGRFGEGYDSAMLHLARVFSEAGFEVIYTELQDPR
                     AIVTSAIQESVDHIGITTLPGADIELFSQIVALLKEMGAERVRVTAGGILPEEDIPRV
                     KAMGVVEFFPRGTTYGELIDWCRKNIRPTDR"
     gene            33918..34136
                     /locus_tag="PLM79_04340"
     CDS             33918..34136
                     /locus_tag="PLM79_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85566.1"
                     /translation="MSAYRIGMDGNLAHHVGHAPMGPHFHGPQWFPHAQTDQVPEVCP
                     CCRGSRLVLDSYNQPQPCPHCFEKTLRR"
     gene            34349..34702
                     /locus_tag="PLM79_04345"
     CDS             34349..34702
                     /locus_tag="PLM79_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85567.1"
                     /translation="MPLEKKEKLMERQWFYKNLQIREGLKPQSELFKYYFIVSDGGRK
                     KCRYCVWIPDDALTQYQETGDFDAIIGAHRDRWSLWVTSKIDAGDFRNLVLKLEKSGE
                     KEYDLDSMDEKLTME"
     gene            35048..35743
                     /locus_tag="PLM79_04350"
     CDS             35048..35743
                     /locus_tag="PLM79_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85568.1"
                     /translation="MSIRILIADDEELVRESIAQFLEHFGYATDVADSCESAWDRLRS
                     ENYDVVVTDKNMPGSDGGREGGMELLRLVKRHQPGAEVIMVTGYGSIDTAIEAMKNGA
                     FDYILKPISPVCLKEKIERIRDYKSYLNPENTVLFYKRIHNQILGLLEGHSALADLDS
                     HEVLKEIETTLDRLFTIQKQYESILLLQKEALGRIAALSEELREAVETLPEVDDATAL
                     LVQRICDEASRRI"
     gene            complement(35811..36086)
                     /locus_tag="PLM79_04355"
     CDS             complement(35811..36086)
                     /locus_tag="PLM79_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85569.1"
                     /translation="MDVKMPVKFHGSYQVVIRYGDVEKKEICQKLNFIRLAAGDEESG
                     APTHQITYYAFGCKRMVEGRIKENREEKVTFDAQGREYEFTPSSRVC"
     gene            complement(36231..37583)
                     /locus_tag="PLM79_04360"
     CDS             complement(36231..37583)
                     /locus_tag="PLM79_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85570.1"
                     /translation="MQTSRINPIQLLVVDDEESIRRLVQRELGGPRRTVRTADTGQKA
                     FDLIRRHQVDVILLDLKLPDADGLDLLDRLREASPDAEVILITGHGNIDNAVEAMKRG
                     AYDYITKPFDLDRLEVVVEKAFQRVCLQRENRLLRHSQRNVPPSHLIGESQVVQQIHY
                     LIDKVAPTNVPVLITGESGTGKDVVAHAIHNRSARADQPFIIKNCGNLQKELVRSELF
                     GYCKGAFTGATASQEGLLALAHLGTFFLDEIGELPLEVQASMLRVLEKQTFRRVGDQQ
                     ERQVDVRFIFATNRNLAEEVKMGRFHEALFHRINVFHIDLLPLRERKEDIPILVDFYL
                     ELLCTGRSPCRVSKEVMQYLMAYHWPGNVRELRNVVERGIILSENGWITPKALPKELT
                     EQKVEEREGPEIVSLDDVERQHILKVLEQVNHNRSQAAEILGIGRKTLYRKLKEYNLA
                     "
     gene            complement(37573..38778)
                     /locus_tag="PLM79_04365"
     CDS             complement(37573..38778)
                     /locus_tag="PLM79_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85571.1"
                     /translation="MEKRKPTLEDVIGIEYVKLGFFREARETIDELRASNQELAQKKQ
                     QIQAILNGITDVMVVLSKDLRIQSVNHVYYETYPDPKPEGKFCHQVFHQLDRPCAVCP
                     AVTAAKTNEVCRKNHIYSVEGRNRHFEVTASPLRNSLGEPAFILLLIRDVTLEKEYQA
                     KYYQAEKMATIGVLASGVAHEINNPLTAISGFAEGLKRRLSRLCQPGQEALIEDFKEY
                     VGIILKECKRCGEIVRSLLTFGHQTSHDFVPVNLNAIVTETLKLLQFHLKRFPQKVVC
                     LDLQELLPPVYGDEPQLKQVILNLMFNALDAIAVQGTITLRTFLENENWVGLAVEDTG
                     CGIPPEHRDKLFEPFFTTKPVGKGIGIGLSTCYNIVTKHGGEIVVTSKEGQGSTFLVR
                     LPRQMKDAD"
     gene            complement(38780..39922)
                     /locus_tag="PLM79_04370"
     CDS             complement(38780..39922)
                     /locus_tag="PLM79_04370"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV85572.1"
                     /translation="MHVLKFSIPEIIFGRDSVQYVGVCARQLGAEKVFLVSDPGVEKA
                     GWVERVVELLKKEKLRWVYYSNVSTNPRDSQVQEGVSLYREQEADVVIGLGGGSPMDT
                     AKGIALVASNGGQIRDYEGANRISYPLPPMLLIPTTAGSGSDISQFAVIRDVERRVKM
                     SIISRTLLPNISIVDPTLLLTQSEDLIAAAALDALSHAVESYVSRIASPFTESQSLQA
                     IQLIMKFARRAVESRELEYLEQLSIASNAAAMAFSNAGVGAEHALAHALGGMFDVLHG
                     WVHPVLLPAVLRFNSSKCLNKMAVIGDTILGKRYGSHQATCDAAIDKLDEFCASLGGA
                     LNLRQIVSDPSLLDDLCRIAAGDVCLLTNPREAGWEDLLGICKEAW"
BASE COUNT         7959 a        12774 c        12247 g         7491 t
ORIGIN      
        1 cggggaaaga gggaatagac ccgctcgagc tcggcggaaa tctcccggga ggttcgataa
       61 taagccccca gctcgaggtt ttccagcacg gtcaggttgg ggaagacccg ccgtccctcg
      121 gggctcaccc caattccttt ccgaatgatc cggaaagggt ccaggcgagt cagttcctcc
      181 cctcccaggc gcacggaacc cgaccggggc ttcaccaggc ccgtcacggc ccgcagggtc
      241 gtggtctttc ccgccccgtt cgccccgatg agggtgacga tccgaccttc cggggcccgg
      301 agactgatgc cccgcagtgc gtggatgccc ccgtaatata cctgcaggtc attcacccgg
      361 agcatcggcg ctgttcccca ggtaggcacg gatgaccttt cggttccgac gaatctccga
      421 cggacttcct tcggcaatgg tgaagccgtg gtccaggacc ttcacccgtt cgcacaggtt
      481 catcacgaac ctcatgtcgt gctcgatgag cagaacggtc agggagtatt tccttctcac
      541 ctcctggacg agatctccca gttcccgggt ctcccggggg ttcatgcccg cagcaggctc
      601 atccagcagg agcagctgcg gccttgccgc cacggcccgg gcgatctcga gccttcgttg
      661 ctgcccatag gccaaaaccc cggccttctc gagggcccag tgggccaggc cgaattcctg
      721 caagatctcc cgggattgtt cccgaatgag cctctcctgt cgtactcccg tccccaggcc
      781 cagcatcacc tgccagaggg gggatctcat gtggtggtga aaggagacca tcacgttttc
      841 caggaccgtc atgtcccggt agaggcgaat gttctgaaag gttcgagcga ttccgaggga
      901 ggcgatttcg tgactgggcc gcccgtcgag gacctcgtcc cgccacacga ccctccccga
      961 ggtgggcttc accatcccgg cgatgacgtt gaagacggtg gttttccccg ctccgttggg
     1021 accgatcagg cccaccagtt ccccggggac caggtgcatg gaaaaatcat tcaccgcggt
     1081 cagccctccg aattgcacga acaggtgttc cacggaaagg aaaacgttca caggaaaagc
     1141 ctcatgatgg tccccggcgg cctcgcagcc aatcccagga aaactcccgc cgccccatga
     1201 ttccgtgcct ccggaacagg atgaccccca tgagcagaag actaaaaatc accatgcgca
     1261 tgccgggaat gcccggaatc gaccaggacc cgaagttcat gggtcgctcc accacccgca
     1321 tggcctcccc ggcccaggtg aacagtgccg cgcccagcac ggccccggta gtgctcccca
     1381 accctcccac cacgatcatg atgagcaggt tgaaggtgag caggaaggtg aacaggttgg
     1441 gagaaatggt ggtgatcagg tgggccagca atcccccggc cactccgctg aaaaaggcgc
     1501 tgaccatgaa ggcgagcaac aggtgtttga aggggtccac ccccatggcc cgggccgcga
     1561 tctcgtcttc ccggatggcc ttcatggccc gcccgtagct gctgtttgtc agtcgggtca
     1621 cgaagatcac cgtgaccacc gcgacgcccc aggaccaacc caggtgagtg tgcggaacca
     1681 ggcccttgag cccaaggggt ccgttggtga cgctttgcat ggcgttgcac agcacccgca
     1741 ccacctcccc gaaccccagg gtcacgatgg ccaggtagtc ccctcgaacc cgaaatacgg
     1801 gaaaactaat gagaaaggcg aaccccatgg ccagcagtcc ggcggccagc agggacacgg
     1861 aaaaaggcgc ctggatcgcg ctcaggggcc acacgagggg ctcgagaatg aaactgagct
     1921 ccttttccgc cgggctcatg gtgagcaggg ccgaggtgta cgccccaatg gtgatgaaag
     1981 cgttgggtcc caggtgaagc attccgcaga ctccgttcac cagattatag ctcacggcaa
     2041 gaatgatgaa tatggccatg ttgttgagga ttcggacctg gtagtccgac ccgtatcgat
     2101 ggaagagaaa cagggtcccg gccaaaagca ggaggcttgc caggttcaac agcaggttgg
     2161 tccgccttcc cgtcggtttc atccgcccgt cctaaacctt ttcctccacg ggttccccga
     2221 ggattcccgt gggccggaac agcaacatca ggatcaaggc cagaaacgaa agggcgtccc
     2281 gatactggga caactgggga accagagcga cgatgaaaat ctccccgaca cccaggacga
     2341 agccgcccag cacggccccg aaaatgtttc cgatccctcc cagcaccgcc gcaatgaagg
     2401 ctttcagccc gggaaggacc cccatgaagg gattcacctg ggggtatttc atggaccaca
     2461 tgattcctcc ggcggcggcc aggacggaac ccagcaggaa ggtgaccgag atgattcgat
     2521 ccagattgat ccccatgagc tgggtcgttt cgaagtccgt agacgccgcc ctcatggcct
     2581 tccccacccg ggtgcgaaag accaccgcga gaagaccgga caacagtacg agggtgatga
     2641 tgggggtgta gagggtgagc accgggatgc gaatgcccat gaattccacc acccggtcga
     2701 atacctccgg tcggggaaag ggcttgggca cccccccgat gacgaccagg gcaaggttct
     2761 cgagcaaaaa ggagaccccg atggccgaga tgagcaggga aatccgcggc gcgccccgca
     2821 aaggccggta ggcggaccgc tccaccagga ttcccaggag ccccgtcccg aggagggcca
     2881 ggggaaagct ccagtaccac ggaagggcaa aaagggtggt cccgtagagg gcggagtagg
     2941 cggcaaacat caggagatcc ccgtgggcga aattgatcag gcgcaagatc ccgtacacca
     3001 tggtgtaccc gatggccacc agggcataga gactccccag ggaaatgccg ttgatcatct
     3061 gctggaaaaa gaccgttgcg ctcaaaacgg ggccccttcc accctcccga ttcgtcacgg
     3121 ctcgatggtc gtcacgtaga cgaacccacc gttctccacc ttgttgatca ccattccctt
     3181 gacgggggtc cctccctccc ccatggagat ggaaccgctc acggcatcga gaccccgggt
     3241 cgaggccagg gcgtcccgaa tccggctgcc gtccgtcgag cccgccctcc ggacggcgtc
     3301 aatgagcaga aaatacgcgt cccctcccaa ggccgcaaac gcgtccgggt cccgcttgta
     3361 ctgttccgta aaccggcgga tgaactcttt tgcccgggga gaaccggccg catcccggtg
     3421 gaagtgcccc gtaagaaaca tgccttccac ggcatttcca ccaatccgga tcagggtatc
     3481 cgaatgggcc ccgtcccccg taaggatggg cgcccggatt cccaggtccc tggcctgttt
     3541 ggccagcaag gcgctctcgg tgtagtagtt gggggcgtaa atgatgtcgg gattggccgc
     3601 ctggagggtg gaaagctggg ccgaaaaatc ctggtccccc gtctggatgt aggccgtgga
     3661 aacgatcttc ccgccgtcct tggtgaactg cttggcaaaa aagctcccca atcccacgct
     3721 gtagtcctgg gcgttgtcga tgatgagggc cgcggtggcc gcttggaggc gggatcgggc
     3781 aaaccgggcc gccacctgtc cttggaacgg atcaatgaag cacgcccgaa aggcgaattt
     3841 cttgccctgg ttcaccaggg gactggtggc ggatgggctc accgaaggaa ttcccgccaa
     3901 ctcggaaatg gggtttcccg ccatggtgtt tccgctgatg gcttccccca ggatccccac
     3961 gaccttgtcc cgctcgatga gcagggtgac ggcgttggcc gcctcgatct tgtcgctctt
     4021 ggtatccacc agcagcagtt caaccttcct gcccagggct tccggttcca tgtcccgggc
     4081 gatccggatc cccgcccatt ccatgtcccc gtaggcggca acggaccccg tcatgggcag
     4141 atacaccccg attctcaggg aatcccggca gtcctcggcc cggacagacc ccgccgtcag
     4201 gcagaaacaa acggcgaaga caaccattgc atgcatgcag gggttcttcc cccatgatcc
     4261 tcgaatccgg cgatgaccca tgctcccttt tcctccatcc ctcgatgtcg caaaaagccg
     4321 ccaagaacgg ccttccaaac tcagccgcaa acgagcgctc ccgcaacccc cctcttagtc
     4381 aatccacctt ttcctgtcaa ccaaacccgg tccttcccgg gtcatcggca gatactaccc
     4441 caattgaccg atcgagggtt gaggaattcc cgggtcgagg gatcggaccg gcacgaatcc
     4501 caattcgtcc cccccatgca agccggattt cgggaagagc tttgatccct tttggatgcc
     4561 gccatcgggg gcgggacggg cggcgggtcc cggatatcgc tcaaccaggg ctttcccggc
     4621 ccccaccctt ccccttccgc ccggggcctg ccgcccggag gacgcgggac ggtccggggc
     4681 actccgttcc cccctcacaa ctcgttgcat cccgtctctc aaagtgatat gtagggatcc
     4741 tcccggcagg gatgcggcgg cgcaggggca ggaaacggac ttgaagccgc ccgcagatat
     4801 tcccgtccgg gtaggcggca cgcctccggt cccacggccg gaacgccgta ttttcgttct
     4861 tactcccttg gtcgtgctag gcggggagct agcggtgccc tgtagcccga aatccgctca
     4921 tgcggggctg aaccccctcc gaagggctga agcggggcct gcaggttcta ataccggttc
     4981 cgattggaca ctgcgcaaca gacgaatacg aaccccgtca ggtccggaag gaagcagcgg
     5041 taagtatcac cggctgtgtc gtggttcaat ctagtcgggt atcggcaggt gagccccagg
     5101 ctgcgacagg ttcgacagag ggtgcacggc caaggttcca ttcctttccc attccccgtg
     5161 cttctctcgt ttcccctctt tcccccggca tcgccgaagg ctttgaggag ccgtgatttc
     5221 ctgttatggt acggtctcgg gagaagattg taacaggatt gccacaggag cttctccgag
     5281 ttgtcacccg gggatggcag acattcccct cgaagacggc ccaggggagg aggaaagaat
     5341 gcccggcacg acggtccttg tggtggaaga cgatcccgat atcctgaatc tcgtttccta
     5401 caatcttcgt atcgccgggt tttcggttct gagcgccccg gaaggccacg aggccctggg
     5461 cttggccaag cagcacctcc cggatctcat cgtcctggac ctcatgatcc ccggcctgga
     5521 cggtttcgag gtttgcaagg agctcaagaa gacctcggcc accaggaaca tccccatcat
     5581 tatgctcacg gcccggggag aggaaatcga tcgcatcgtg ggcctcgagt tgggagccga
     5641 cgactacatg gtcaagccct tcagcccccg agagctgatc ctgcggattc gggccgtcct
     5701 gcgcaggacc gggtccgacc ggcgcgacga gaccctgctt cagaaggaag gcatccaggt
     5761 aagcctcgaa tcccacaagg tctgggtgga aggaaacgag gtgattctca cggccacgga
     5821 attcaagctc ctggtcgagc tccttcgcgc tccgggaaag gtacataccc gggaccagct
     5881 gctggacaag gtgtgggggt atcatttcga gggctatgcc aggaccgtgg acacccacgt
     5941 ccggcgcctg aggcaaaagc tgggcgttta ctcggaggcg gtggagaccg ttcgaggggt
     6001 gggataccgc tttagggacg cgtgaagcca tgtccggaag gaagtctctc aaaaattcga
     6061 ttctctacta ttgctgggca ctggtcctgg cgggggtcct cccctgcggc tacctgtatc
     6121 acctgcttag ccgtaccgcc gtcgaggacg ctcaaggcat ggcccttcga gaactggacc
     6181 tggtggagcg gctcttgacg gacgccgccc ccttcccgaa ggtcgaggac ctgcaagacc
     6241 gagtgacccg tgccgcaaaa cagcgagggg ttcgaatttc ctacgtggcc gaggacggac
     6301 gggtccttgc cgactcgatg accgacccgg accggatttc cgatctcgag aacttcgcgg
     6361 gcagggccga gatcctcgat gcccgcagcg gcgcccccgg cgtctccttt cgctacagcc
     6421 aggccgtgaa aaaggaaatg gccttcgccg caaagaaaac cgctctcctc gagggtcatc
     6481 ctcccgggtt cctgaggctt gccatggatc cgccctcgat ccaagggcaa ctggatcggt
     6541 tcattcaagc cgttcccctc tacctttttc ttctctgcgc cgtggttgcc ctgtccgtgc
     6601 ccctggtggt gcgccggttc gacggaatcc tgtcggaaat gacccgggcc gtcgattccc
     6661 tggggtccgg ccggcccgcc cggcgagttc acctggaacc gggtcatgag ctgtctcccc
     6721 tggccctgtc cctccaacag tgcgccgaga gactggaaga tcgcctggcc tccacgaacg
     6781 aagaaagacg gcgcctcgag gcggtcttcg agggaatgcg ggaaggagtt atcgtcctcg
     6841 actcccgggg aagaatccgg agcgtgaacc gggcgctggc gaacctggtt ccccgcacgc
     6901 ccagtcccgt cggtcgcaag cccatcgagg tgatcatgaa cctcgagctc caggaggcct
     6961 gccacgcggc gttgagcccc gatcgtccgt caaacgaaag ctttcgcaga ctcgagctcg
     7021 tcctctggag ggagcgatcc tacaccgtga ccatctcccg gttgcccccc acccgggacg
     7081 agggggaaga cgaaatcccc ggcgccatcg tggtcttcca cgacatcacg gagatcaagc
     7141 ggctggagaa ggtccgacag gatttcgtgg ccaacgtttc ccatgagctg aggacccctc
     7201 ttacctccgt caagggatac actgaaaccc tgctgtccga agagatgccc gaccggcaaa
     7261 ccctcgcctc ctttctacag gtcatcctca agaacaccaa ccacatggtg aaaatcgtgg
     7321 acgacctcct cgagctggcc cgcctggaag ccctgcagtt ccccgtccgc ccggttcccg
     7381 tccatgcggg aaccgccctt catgcggcat ggaaggcctg tcttcccctg gcctcggccc
     7441 gggagatctc cctgcagaac gatctgcccc cccacggtcc gtgggtgctg gcagtcccgg
     7501 accaactggt ccaggtcttt cgaaacctga tcgagaatgc cgtccggtac aatccggcct
     7561 caaaacccat cgtggtgtcc tgcgaagaac aagaggaaac cgtgactttt cgggtccgcg
     7621 actttggacc ggggattccc aaagaacacc aggaccgcat cttcgaacgg ttctaccgag
     7681 tcgaaaagca ccgagcgggg gaggcgggca gcaccggcct gggtttggcc atctgcaggc
     7741 atatcctgcg aaacctcggg ggccgcgtgt gggtctgcga cccttccccc ggcaggcagg
     7801 agggagccgc cttctgcttt acccttctgc gggccccggc ggaggcgaac aggcgggagg
     7861 gctaggaccg tggaatctgg gaggccggga agccgggagg ggtgtagggt gggtggagcg
     7921 aagcggagcc caccgagggg gcgggaagct gagaggccgg gaagcgttgg ccgagttctt
     7981 ttcccctttg ggtccccgaa agggtcggga acccaaaggg gaccgtcgcc cggcaggaaa
     8041 actccccctg ccccggctcg attcaaacgg gcggaagacg aacccatcga ttttgtgtac
     8101 agttcttatg gagaagcccc agcggcttcg gcttgggatc ttgatcctta ccccgggttc
     8161 gacggcggcg ttcccatcgg cggacacggc gcctcccgga gggtaggaag ggttgtggaa
     8221 tggaaaaatc ccccaagatg gcttcggaaa gcctgaattt ttactacggc gctttccagg
     8281 ccctcgaaaa catcagcctc gagttttacc cgcaacaggt cacggccctc atcggcccgt
     8341 cgggatgtgg aaagagcacg tttctacggt gcctgaaccg aatgaacgac ctcatcgcca
     8401 ccagccgggt cgaaggccgg gtgctcctgg accaggagga catctacgat cccaagctgg
     8461 atgtggtgac cttgaggcgc cgggtgggca tggtgttcca aaagcccaat cccttcccca
     8521 agaccatctt cgaaaacgtg gcctacgggc tccgggtcaa cggggtgcgc gaccgttcct
     8581 tcctggccga aagggtggaa ttcagcctcc ggagggccgc cctctgggac gaggtgaaag
     8641 accggctgca ccattcggcc ctgggcctct cgggcggaca gcagcagcgc ctgtgcattg
     8701 cccgggccct ggccgtggaa cccgaggtcc tgctcatgga cgagcccgct tccgccctgg
     8761 atcccatcgc cactcaaaag atcgaggaat tgatccacga gctgaagggt tcgtacacca
     8821 tcgtcatcgt gacccacaac atgcagcagg cggcgagggt gtccgactgc accgccttct
     8881 tctacatggg taagctcgtg gaggtgaacg agaccgaggt cctgttcacc cggcctcagt
     8941 atcgccagac ggaagactac attacgggcc gattcggata ggagcgccga aaatggaaaa
     9001 ccggttccac caggaaatgg aagagctcaa gatgaccatc cttcacatgg cggtcctgac
     9061 ggaaagagcc ctggaaaacg ccgtgcggtc cctcgtggat cgggacggcg acctggccca
     9121 ggcggtcatt gacggggacc gggagatcaa cctccgggaa atggaggtgg atcgccgatg
     9181 ccttcgcctg ctggccctgg accagcccat ggcccgggac ctgcgcttca ttgtgggctg
     9241 ccttcggatt tccatcgaac tcgaacgcat tgcggaccaa gccgtcaaca tcgcccagag
     9301 agcccagttc ctgggaagcc gcccccccct gccccggaac ccggccatcg aacaattggc
     9361 cgacacggcc ctggacatgt accgatccgt catgtccgcg ttcgtcaacg aaaacacgaa
     9421 cctggcccgg gacgtctgcc agacggacga cctggccgac gagctcaacg tcacggtgct
     9481 caagaacctc ctggactaca tggtccacga ggtccccgcc gtggaaagat ccgtgcagac
     9541 catcatcgtg gctcgatgcc tcgaacgggt cgccgaccag accaccaata tcgccgaatc
     9601 cgtcatcttc atcgtcgagg gggtcaacat caagcaccgg tgccagcagt aggcttgcac
     9661 gcccggactg gtcagaaacg gcgctggagc cgggcccggt agtagatggc cacgaggttc
     9721 atgccgaaga ccatcaggat gagggtgagg gccgtgccgt actggagggg tcgggtggct
     9781 tcgatgttgg tccccgcggt ggcaagcaca taaatgtggt acggcagcgc catgaccggg
     9841 tccaggaggg acgagggcaa gtggggcgtg aagaacaccg cggcggtgaa catgatggcg
     9901 gccgtctccc ccgcggccct gctcacggcc aggatggccc cggtgagcat tcccgggaag
     9961 gctgccggga ggaccacccg gtagatggtt tgccacttgg tggctcccag ggccagggac
    10021 gcttcccggt aggtggccgg gaccgccttc aaggcctctt ccgccgttcc gatgatgacg
    10081 ggcagcacca gggcccccag ggtcagcacc cccgcaagca cactcactcc caggcccatc
    10141 cacgtgacga aaagagccag tccgaagagg ccgaacacca ccgaaggcac ccccgccagg
    10201 ttggtgaccc ccaggcggat catgcgaacc ggcattcccg gccgggcgta ttcgtgcagg
    10261 tagaccgccg acgccactcc ccacgggaag gccaccacca tggctccaaa gctcagcaga
    10321 aaggttcccg caatgcaggg aaagatgccc cccgccgtca tggaattggt gggggcctgg
    10381 gtcaggaagg tccaactgac ggatctccat ccgttccagg ccaggaaacc caggatgacc
    10441 aggaggacca gtccgttgac gacggccgcc ccgcgaaaga gggtgaagac gacaacctgg
    10501 agccgacgcc gaaacccggt tcccgaaacg ggccgtgcag gaccgggtcc cgacccgcga
    10561 cccacggcct ctctccccga gtctcgggaa ggaccaaaga ccgacgacct ggacgaaaac
    10621 acccgggacg gaaccatgca ttcccctttc ccgccgccca tcacagggtg gccgcaccca
    10681 cctgtttgtg tttttgagcc accatgtccg ccagcaggtt aaaaaggacc gtgaagagaa
    10741 acagcacgat gcccgtggcg aacagggcgt agtaatgatc gctgcggaag ggagcctcgg
    10801 ccatttccgc ggcgatactg gccggcatgg ggcgtacggg cttgaagatg gaagtgggga
    10861 ccagcgccgc ccccccggcg accatgagaa ccaccatggt ctccccgacg gctcgagaca
    10921 tccccaggat cacccccgtg ctgattcccg agagggacgc cgggaagatc acccggtaga
    10981 tggtttccca gtgagtcgcc cccagggcca gggacgcttc cttgagctcc cggggaacgc
    11041 tgtagatggc gtcttcggaa atgctgcaga tggtgggcac cgacatgaag gccagcatga
    11101 gggacgcatt gaacaggttg agacccgtcg ggatttccag gtaatcctgg agaaacgggg
    11161 ccatcaccac catgccgaaa aaaccgatga ccacggacgg cagcgcggcg agaagctcga
    11221 ccacgggctt ggcgatctcc cgcatgcggg ccgaagcgat ctcggcgagg tatatggccg
    11281 tcatgacccc caagggaatg gaaatcaagg cggaaaacgt cgtcacggca acggaagcga
    11341 ccagcagggg gaagatcccg aagtcggggg gggaagccgt gggataccag tttgtgccga
    11401 agagaaattt ccacacggaa accttcgaga aaatggggac cccctccatg aacaggaaca
    11461 cgaggatgag gaagagaacc aggatggaaa caaaagccgt ggcaaagaag aaccaacgaa
    11521 tgaacgtttc tttgatctgc cggtgagtca tcatccttct atgatcccgc cctgggttga
    11581 tccacggatg ggttttccat cctccgcctc cctctcccct cgaggccgtg gaaagggagt
    11641 ttcccgctcc aaaaaggccg gcaggagcct ccgtctcccc cttgggagag aggggccggg
    11701 gggggcaatc cacctcctat gcatcgcccc ctgccctagt acaagggcac aaatccggct
    11761 tccttcacaa gcttctgacc cttatgcggg tcgaggacat agttgacgaa atggagcaca
    11821 tctcccgaag gccaaccccg ggtgaacatg aaaaggggcc tcgagaccgg gtatttcccg
    11881 gtcagagtgg tttcctcgtt cccctcgatc ccgtttaccg tgagggcctt gacggagggg
    11941 ttcaggtacc cgattccgag atagccgatg gcgaacttgt tcttggccac cgcctggacg
    12001 atggcgccgc tcgaagcctg gagcatggcc ccggcgtaaa cccgggcctt cttcatgacc
    12061 ttctcttccc agacctcgta ggtccccgac gaggtgtccc gggaaatgac cacgatctcc
    12121 ttgtcctgtc cccccacgtc cttccagttc ttgatctccc ccatgtagac cgctttgagc
    12181 tgatccaggg tgaggttctt cacggggttt tccgggtgca ccaccgggac gatgcagtcg
    12241 taggccaggg cgaaggggac ggggtaggcc cccttggcta cggcggcctt gacttcctcg
    12301 tccttgatga accgggaact gttggcaatg tccgtggtcc cgtcgatgag ggccttgatt
    12361 ccgttgcccg accctccccc cgacagggag atggagaccc cggggttctc cttcatgtag
    12421 gcttccatga ccttttgggt gatgggcaaa accgtggtgg atccatcgat cctgacggaa
    12481 cccgcatggc tcaaagttgc cgcaaaaatc ccgcacagca gtgcgatcaa aaagacccgt
    12541 cctttgcctt tggatctcat gccgtcttcc tccttgcttt acggtgattg gtggaattcc
    12601 tcgatctcat ttcccaatcc atggcctgca ttgaatggtg actcgaaaat aggcaggccg
    12661 tgttacgatc ccgtgacaaa acccgggaga ttccatgacg gagccgagac aagtccggat
    12721 cttcccctcc cggggacacc ggcagcgtct tcactcccga aggaagcggc gtacgggaca
    12781 cgcatttccg tggagccgta ccgtgccgac tcgggggttc ccccggcgca acggtgcctg
    12841 cctcgatcct cgtcccgggt ttgtgaaatt tattttcttg acgatctgaa atatttggat
    12901 ctttttcgta agtgtgcgag gaagtaggca actgcaaccg attcacgccg gtcggcaccg
    12961 atccgctttt tcggctccct gcggttctcg aaatcacact ctcggaccct cccttccggg
    13021 ggaggaatct tcgcccgtca accatgatcg gttgacgact ccagtccctt gctccgctga
    13081 atcacggcct ctcttctccc gtttccttgg tgagctccat ccgcctcaag cctgaagccc
    13141 ccgactccac cgggaaagaa cccgttttcg cacaaaaaga aggttgccgg gtcccaaggc
    13201 ggcatccctc ccaagggttt tgcggggtct tccctggatt tcccgccgaa tgccgggatt
    13261 ccttcaagga ccgggtaccg gctttaggga caagtgtccg cgcatcttcc cgtttggagt
    13321 gcttctgcgg tttcttcggt tgcccgcagc catgacgctt catggatccc tcggttcccc
    13381 gaaagacccg gggcgggtcc cctcgacttt ccgggcgggc tcgaggatga aagagagagg
    13441 aagcatggca ggaaccaatc gaccacggct aagccgacgg acccgggggg ctgaaaaagg
    13501 tcttccctcc accgcccatt ctcctgggga ttgggccctg gttgaaaact cgtccccggc
    13561 gttcctggcg gttcgaatcg gcggtgacct gtgccgggtg attgaccggg aatccctggg
    13621 gaaagacctt tgtttgcttt tgatcggggc ccgttacgca caaacctgtc gggagcgtgc
    13681 ctggcctccg cccctggacg gccttcttcg gcaggaggcc caccggagcc gctttcacat
    13741 cctgtgcctc cgagaagatg cccaaccggg aaagcttctc cccgtgggga ccctcatgga
    13801 ggattccacc ggcgaccggc atgtgatcga ggtatccccc cacgcaaccg gtctcctggg
    13861 gcccatcctc ctcgccgccg cggcggaact gtcgtgccgg gcgcaagatc cctcccgcta
    13921 cgactctttc ggggacttct tgtacccggg aggccccaat ctcgtggaaa gacacagcac
    13981 ggaacgactc caaaagcacc tgggacgaaa agtggtctac gacgctcttt tgtgcaacca
    14041 ttgccttcgt tgcgccaccg tctgcggtga aatgaaggcc gtcaccgccc ggggcaacct
    14101 gcagttgctg ggacccggcg agcacttttg cactgcgtgc gggttgtgtc aaatgcgatg
    14161 ttcctacctc gccaccatcc ctaaggaagg ttcctctcgg tccgccgacc cgcgacttcg
    14221 cctgcatgaa gggggagcgg ccctgcatct ctacggggac acggctttcc ggttccggga
    14281 ccttctgcgc cgtacggtgt cttccgggga ttccatcgac ttcccgtacc gggtttcccg
    14341 cttgtgggag atccccccgg acggggagat tcccccggaa atccctcttc agttcaagat
    14401 cgaggcccgt gaccccgagg ggggacttcc cggccaagcc cttctggcga ccttcccggg
    14461 agacggctcc gggggagaaa ccgccgtggt gcgcagctgc cgtacggcag gtttggtgct
    14521 gcaaaccgaa gaaggagacg tggaacggga catcgccggg caggccctcg ccctgggtct
    14581 cgaggtgaag ctgattctcg attgcgagcg caccggcatt tccgttcccc ggaacccgct
    14641 cacggaatgg tttcaccggc tggggcaacc cgccctcgtg ggaaagtctc tcctccaagc
    14701 ctgggaatcc ggggaagtgg atttcttttt ggccccctat gccgacgagg tctcccccga
    14761 attgcagcgt cgaatcctgg aacaaacggg gaggacggcc cacatcctgg cccccaatct
    14821 cctccaggcc cttcccttct cgaagagccc tcttctttgg caactgaacc gaagcctttg
    14881 gaaagtgtac ctccggcacc cggaactgct cgcaaaggag gcccggtacg cctcggagct
    14941 tctcgagaac cttcccgcaa actcggcggt actccacgcc cgctatcgtc ccctggcctt
    15001 tgcgagcggg cattcggcct gcccctcctg tgcggaagcc atggtgttgt cgatccccgt
    15061 ttacatggcc attgccatga gtctggcccg cggagagatc ccccgggttt ccttcacctg
    15121 cgaaacgggc tgcatgagcg agaccctgaa caaggtcggc gaggtctctc aaaaggtccc
    15181 cggaggacga acggtattcg gaggaggctt cgccttcgga gaagccatcg ccatggtcca
    15241 ggaccatgcc gtgcgccggg gggttcttcc caagggccgt cgatacgtca tcagtcaggg
    15301 aggagacggg ggcgccgtca tcggtcttcc ggcgtggctc aacgcccttc gccaacaagc
    15361 ccgtctgatc cggcagagac accccaatgt gctgcatttc atcaatatta cggataccca
    15421 agtctactcc aacaccgggg gggagagttc cgccagctcc ctgctgggaa tgggcactct
    15481 cacgaccccc atcggccgct ttctgctggg aaaccagagg acccagtgga gcctgatcaa
    15541 cctggccgcc gagtttcccg ggatcctggt gggggccgga cacagcgcca acaagctcgg
    15601 tttgcagcag ttctggcacc tggccgatca gctgggcgag tcggccattc actgggacat
    15661 cagcccgtgt cccgagaccg ggaaattcta tggggaggac ccggacgacc tggcggaact
    15721 catgagccat gcggggatga tcccggaaat ccttttcgtc ggccggttcc gaaaacgggt
    15781 ggccccttac catcccgagg accggaacaa gccctaccgg gaatggcgcc tgcaaccgaa
    15841 gcccgttctt tactggctcc gaagggaccc ccggtatcgc atgctcttca agaccaatcc
    15901 cgagaccggg gaaatggagg ttcgaaacct cacctgccac tacctggtaa cccagatcga
    15961 gagctaccgg gaccaggtca accggcagat cgacctcgag agccggatcg ttcgacgggc
    16021 ggaggagcgg gtttcggaat tctttcaaga gctgcgggag tcgtgggagc actatcgatt
    16081 ccgcctggga gagttcccct atgccatgct cttcaacgat cggggcgaac gaaaaccgga
    16141 atacggcatc ggtctcgagt acgaaatggt gcgaaggatt ttggggtggg aggaagcaac
    16201 cggggtcctc gaccggcgcg accggcttct aaaccatcaa aggagccggt tggaagaact
    16261 cgtttcggaa ctggaaagcc tcgatggcgg gtccgtcgca cagatcgtcg agtcggaccc
    16321 cctggcccgg cgtcatggag ccgtcaagga ccttctaatc caaattcaaa aaacggagga
    16381 gatccttcag gaacaggccc gagggctgcg cgcagccgtg aacgggaccc tcgctcgcga
    16441 tcccgtggag gacgaaatct tcggcccgcc tcccgattcg gtcgaggccc ttgcccgaga
    16501 ccgcatgcgg gacctctacg gcctcctgga cagaatcatc gaggaacgcg ccgttgcccg
    16561 tcaggttgag ctgcagcaac atcgcctggc ccggaccctg acccgcaaat tcgaggagga
    16621 aggaggggtc ttcggccgcc ccagagcccc cgcccatgcc gagaacggcc gcgagctcct
    16681 cgatcatatc cgtcgcttcg ggcccttttc cgtgggagtg gccagcctgg ccggagatcg
    16741 cggcatcgcc gtcaaccgcg tctttgcggg gtttttcagc gccaaggggg cctgggcggg
    16801 catggcctgg cagttcggct cttccaagag ggggaccccg gtgctctcgg cgaccttcgt
    16861 ggacgcccgt ccctttcaga ggaaggacgc catgcagagc gttcccgtgg ccgtgctcgc
    16921 cgtgaccaat ttcgaggagt tgaagcgaag ccccgatctg ctctttggag gaatgcgccc
    16981 ggggggctac ctcatcatca accaccgcaa gagcgcccgg gctctctggc acgagctcgt
    17041 cgcgtttttc cccgaagagg tgcggacctg ggtcgccggg gccgtcgagg cgtcttccgg
    17101 aaagccgatc tcctccccga ctttccctgg ggggaatagg gaggagcacg gtcctccggg
    17161 cctgccggga agcggcacga cctggacgga cctttcggag ccgcttttcg gaaagccctt
    17221 ctccctcctg actcccggtc aaaaagagtg gaccaggaag gttgcggcca tctccacggt
    17281 tcgaatcctc agcctggaca tggacgggat catccaggaa gtcacgggat ctcggaagat
    17341 cgtctcgaac ctcgtggccg tggccccgct cttccatgct ctccaggagc agggatttcc
    17401 tttccgctgg gaagaggatc gggacctgct cgtccggggt tttcccgaag cggtcctgaa
    17461 aaatcctcaa ctgctggcac agtacgagcg gggcatggag ctggcccggg aacggtggaa
    17521 ctcatggacc cccgtcccgg catcccccgc accgccctcc cccgccgaaa gacgggaccc
    17581 gtcgtcattg gtctcggcca aggcggagga ggtcccccgg gaagaatccc cctcggattg
    17641 cctcatgatc atggggggca ccctggccgg gatggtcctc agccaggtgg ccctcgagga
    17701 gcatcccctt ttctacatcg gatttcccat caccccggcg ggaaacccct tctatgccat
    17761 ggctgaagcc tatgccgacg gccaccccta catcgtggtg gacgaggtca atccctcgga
    17821 aaaggtggcg gcggagaagc tgttgggaat cgcccgtacg ggaggctttc tgcccgtgac
    17881 cttcaccgcg tcccagggat ggaggctgtt tacggaaatc ctgccccaat tcgtgggcgc
    17941 acgcctggaa gggcttttca tcatcacccg gagggccctg gccgctccca acctcaatat
    18001 cgaggaaagc cacacggatt tcctcagttt ccgggacgac gggggcatca tgttgtctcc
    18061 caagggcatg gaagaatacg tgcccacgtt gtacctggcc cggattctga cccattttgc
    18121 caagcttccc gtgatcctat ccatcggagg aattacggac acccacaaaa tcgggctgtt
    18181 caaagtgcct cccgacgaag aggtgcgcac ctggctgcga aagacccttg ccgatttcga
    18241 tttcctcgag gacaagctcc tcaacccgca ggagcacctc atcgtgcacg gacccagcgg
    18301 cacatcgagc gtctaccagg aaacccagtc cgaaatcgag aaggcgcacc gggcggtgca
    18361 gcgaatcttt ccttacgccc tgcgggccgt ctcccggctg accggaaccg cgctgagcga
    18421 gatcgaggtg gccggaaccg acccatcgcc ccagaggacg gaaaccttac tgattctcca
    18481 gggatcgctc tttcccaacg ccgaggcagc cctgctggac ctcgagcggg aagggtggag
    18541 gggccttgcc tgcatttccg tccgatgttt caatcccttt cccgaagagg agctcctgcc
    18601 ctggctgtcc ggagcccggc ggattgcggt catggaccgg tccaacagct tcgggtccct
    18661 tcctcccttg gcttcccgcg tattttccgc cgtcgcccgg catttgtccc gtccatgggc
    18721 ggatcatccc cggcaacttc gttctctggt gggaggcctg gggggaagaa tgattaccgt
    18781 ggcgcaaatg cgggaaatcc tcctttcgac ccacctcctt ttccacccgc cccgggagtg
    18841 ggaaaccggg ctggtgcggg aatggatcgc ctccgatgag accttgaggg ccctcctcga
    18901 ggaggcggcc gccctggacc tgagaaacac cgatcgtcat tcccgggtgc ccgagtctct
    18961 aagacctgcg tcggcgcgac ggcgggaata cgaggagcgg ctcgccgccc tttcgcgaca
    19021 actcctggaa aaggattacc gatcccttct ggccaactac caccaggtgg aattcatcga
    19081 accccgggaa gtcctgctgg aaacgaccct cctccaacaa atcgtgcagt atgtggagac
    19141 ccgcctggcg cggcatgccc tggacaacgg cctcgcatcc ccgcgccacg ctcagctctt
    19201 gaatgcctac tcgacgagcg aagaggacct tgaacgggcc agggtatacc tcgagggagc
    19261 cgccgaggcc cgggagggtt tccccggggc ggtttccgtt tcgggggact cggagataca
    19321 ctcctcacct cctgcggaag aactgaagga aaccgagccg ggagacccca ttcccgaccc
    19381 tcgatccgac cggtccggcc ctctcccggc cggggagccg gatgaaccgg cggacttcgc
    19441 gccggacccc cgggaggccg aactcatcga ggttcatctc acctcgctgg cggccctcca
    19501 gggggagctt cccctctact acaacccgga agacttcgag cacgaggttc tgcgccttct
    19561 ccagaaagat cccgggtcgt gcttgttcga attgagtcga aaggttcccg ccgaccggtt
    19621 gccccggctt ctccgtcgtt accgctcctg ctacgcagat gtgatcgaca gggttctcca
    19681 gcgggaggtc ttgtccatgc accacgcccc cgagcttcga ggcctgtttg aaggagaagg
    19741 gaaaaggaga ctggaagcgt tggcgttacg gattctgccc tatggggagg ggatggaccc
    19801 ggaaaggctc gagcggtggc tcaccgggga actcgagcgg tacctggcgg aggtcctcct
    19861 gccgggctgc cccaaggcgc ccatattcta cctggagtac tttcgccaat ggattgcccc
    19921 ggacctggtc aaaaccgccc tggaccccct tcacgccgca cgaaccaagg aggactttca
    19981 ggcctaggac gtcccatctt tccacccctc ttccgccggg ggcactgtgg tttgcttgag
    20041 gacccgcgaa ggctccctcg ccccccttcg ggggagaggg tcgcggagag gcagtctttc
    20101 ctcctccctc gatggaagga actggcggaa ggcagaaccc ttcggggaag catttgttcc
    20161 aggtcatcga gtgaatatcg gcgggttttt ccaagaaccg tatttgctcg aatcggtcac
    20221 tttcctcgag aaggagtccg aaccatgaca aacgagccct tgatcgtttc tttcgaaaag
    20281 gcggacccca cccaggtgga caggctggga gccaaggggg ccaagctggt ggaggatttc
    20341 aggcacgttc cggagatgtt tcccggcatg gaggatacca tctcggtgcc cgacgggttc
    20401 atcatttcga ccgatgcctg gaggcttttc cacaagggag gagaccgcct caacgacgag
    20461 cttttcgccc gaattctcct ggatctatcc tccctcgagc gtcgcgtggg aaagcaatac
    20521 ggagcctgta ccggccggat gccccttgcc gtggcggtga gaggcggggc gcccatttcc
    20581 ctgccgggag ccttgggcac cgtgctcaat gtcgggttga acgacgagct ggtcgctcac
    20641 ctgattgaag cgggcatgga cgaaagcttc gtattgacca cctatcttac ggccatccgg
    20701 atgtacggcg aagttgtcct ggatattccg tacgagcctt tctacgcggc gcttcgtcga
    20761 taccatgtgg gggacgaagg caccattccg gtgccggtct tgcactcttt gctcatcgag
    20821 ttcaagggaa tcctcgatcg gcacagccac ccgcggtttc cctcgggatt cgagcagcgg
    20881 ccggactatc agctgcgcta ctgcatcgaa ggagtgctgg catcctggat gtctcctacg
    20941 gccgtcgaag cccgtcgaag ccgccggggc caaaccggag ggatctccga cgacatggga
    21001 acggcggtca tcatccagga gatggtgttc gggaaccgaa acgatcacag ctgcctcagt
    21061 ggggtgttgt ttacccggga cccgcgaacg gggtcggaca atttcctcat cgagtgggcc
    21121 ccaaaggtcc agtgtgacaa gatcgtatcg gggaaacttc gcaagcagct ccttcatgcc
    21181 gacgatctgc agaaacgctt tccggacgtc caccggctgt tgtccctggc cagagaccgc
    21241 ttcgagacgt gggcccggcg tcccctggac attgagttca ccgtggaaga caaccacctc
    21301 ttcctccttc aacgccgtcc cctgcgcatg acttccaacg ccacggtccg ggccatgtgg
    21361 gacctggtgg acgagggaaa gaccaccatc cagctggcgt ccctcatcat caacaaggcc
    21421 ctcgaacaac ccgagaaagt cctcaagagc gggttccatg actacgtggt ccttgccgtg
    21481 ggggaaccca tcaccgacag cgccgacagc ggcattctca cggtggggac cgaagcggcc
    21541 ctcgaactcg cccagcaggg ccgggaggtc attcttcttc gacggaatcc ttacggggag
    21601 agcgatgtgg cagtgaatca tcccaatgtc cggggggtca tccgttgcga cggcaacgcc
    21661 acgggacatg aagccgtttc cgccgtggcc tacagcaaac cgtacctcat caacatggcc
    21721 gatgcttccg gatgtcctct catttgcgga gacggagagg agcggatgct caacccggac
    21781 agcctcctgg ctcgctatgt cggaaagcgg gtgtttgtgg acggggaaca cggcatcctg
    21841 gggtatacgg aacacacgga tttcctcgag gaccgaaaga gccgcaagag gttgtacgtg
    21901 gattgggaat atctgcagga gcggttcgac gacggccgat acgatcagtt gtcctacgag
    21961 gagctcctgg acctccacta ccattgggag ctggagctgg atcaatacct gcagctcgag
    22021 aagtcgcttc gggacgggga cccctcgatc cctacggagg ccctgttgca ggccttcacc
    22081 ctttacctgg cctgcatccc ggaaaagaag cgggatcggg tcctgcatct aaaaggcgtc
    22141 aaggtcgaag acttcgtttt cgaaccctcc gtgcgatacc tgggcaacca tctggggcag
    22201 gaggtccgaa aaattctccg tgccctcatg ctctgcacca cctggcggac tcactggctc
    22261 cacgaaatca tggttcgcct tgcccgggaa cgaggggaaa cggaaaacga cgtcattcga
    22321 gacattttcc tcaagaatca aaccatgagc ctggtgaggg atttcgagga agaggggttc
    22381 cacctcatga aggtttctcg atactatcac ctgatcctcg ccagcaattt tgagtacggc
    22441 caggacctgg acaaggttct ggtgggtccc ggcgccatga attttgccga gaaggaattc
    22501 ctggcccggc acttcctcga cttcctgaac gaggtgaatc cggagctccg cagtcgagtg
    22561 cggatggttt cgggggaacc tcccttgggc caggggcatg cacggatcat cagcatcgga
    22621 ctggccattg tccacgagga tttcggcctc gtctgccgat acctgcgatc cttcctcgat
    22681 caacggaaac agggcatgct ggcaagactc ccgacccgga ttcccgtctc cgagttcgtg
    22741 gaactctacc atctggatcc ttttttcgcg ccctttcccg acctgagaat ttcccgggaa
    22801 gcttccaacc aactccccac gggggacggg ctcctgacct ttgggagttg ttcctttggc
    22861 gagtacgagg gttcgttcta cgggaaggaa gactatgatc ggctgggggc cgaagtcaga
    22921 cgattcgagc agtacctgag aaagtccggc cgtaccctcg aggttcgccc atggcagttc
    22981 gaggtggatc cctaccgacg ccactcggtc attgcggccg tgggaattcg cttcgatctc
    23041 gaggagctgg acgtcctcat ggaatccctc aagggattcc tcgtcccgtc cactgaaaag
    23101 cctgcacccg ggaccctcga ggtccaaggg cttcgcccgg agaatcgagt gtgaccatgc
    23161 ttaccgagga aatgcgacgc actatctatt ccgaactgga ggccgccaac cccgccgccg
    23221 ggaagccttc ttccccgaag cacggaaagg tcccggcact ccccggtccg ggcgcggatc
    23281 tgcgcgccca atgggcccaa gaagtccacg cacgaacgga gatcgtcctg gacaggctgc
    23341 tgtttcaact gcactccctc ttcgattttc gggaagacga atcgcggatc caggccgtgg
    23401 tgcgccgggc cctctcggaa gtctacgaca tcgagaactc ctgtcgtgga gacggatcct
    23461 acgatgcctt ctcgtatgcc ctgtgcctct ttcggaagag gtggcacgaa gggagccggg
    23521 gcgtgggaga ttccggcctg agcgggcttg ctcacgtgga cagggcgcaa ttcttttcca
    23581 aggtccccca ggagatccac acttgggccc ggcgctttct cctggtggaa cggtaccatc
    23641 gcctgcatcc ccgggaaggc gagaaccgac tggaccgttt ccgcctcttg cgctcgccgg
    23701 gaccctacag cgagatcacc tccttggacg acttcctggt ccggctcgat ttccgcttca
    23761 tggaagaatc cggccgagag gaccccgatg tgggttccat ggctcgccgt cttgcggcgg
    23821 agatccaagg acgattgtcc ccggacctgt ttcccggtga gccgctccaa cccctggtgt
    23881 atctcttcct gaggtgtcac cgggagtgtc tgagcgaagt cgtggaaccg gaggagaaac
    23941 gccgcctcga ccaccttcat tccactcgac agatggacag ccttctggac cttatgcgcg
    24001 tggtcatcca ccgaacgacc cgggaacacc gcctgcccga agccgccggg atcctggatt
    24061 ccctgaagga ggaaggcttc aacatttccc atgccgcttt tcgggacccg tacgaagcgg
    24121 acctcgtacc tccggccgat accgagcggt tcgagcaaag cgggctgcct ctttttctcc
    24181 ctctcgattc actcacgggg gacttttccg cggacctcga gtggagcagg aagcccatcg
    24241 aggaatggaa ccaacggatc gtccgagaag tttccctcga ggtggaagag tattgccggg
    24301 caagcagcca accgctgccc cgggcgcgac tcgttctcaa gctcatacgg tttgccgtca
    24361 gccgagccct ggctcgaacg ccgtggaacg agatcgagtg gaaggaagac ctgccgacca
    24421 aggtcctcga gtggattccc gggccggtgt tgcgaagcct gattcgccct cgaaagggct
    24481 atgaaatcaa gtccaagcag gaactggttc agcggatttc catggaatgc aggcttcagt
    24541 gcgaggccct cgagcgcatt gcccggaccc gtcggatcga gggataccac gactatcgtt
    24601 atttcggccg gtatgtcctt ttccagggta tccaaacctc ctcgagcgcc ccgttgggcc
    24661 ccgaggttcg ggaactgcgg catcggctgc cgcatctgcc ggatccccgg tggctcgagt
    24721 gtgccggaaa ttcttacggc ctcgatatct ccagcgtcca tcctcatacg ctggactgcg
    24781 tcccccgata cttggagggc cgggtgcctt tgtcgatgct ggtgtccgaa ctcgagaaga
    24841 acttcgagac ctccgaaaac gagctttacc gggttgcccg ccacaccctt cggagcctca
    24901 cgccttcctt ccatcggctg ctggtggcct ttgcccgccg ccgtgcccgg ctcccccgac
    24961 cggagtgcag cccccgagac atcccaggct tgatccaact cctcgcccca cccctgcttt
    25021 ccctcgagga cgacgctcgt ctccacatcc tcggccacct tcgtcaacac gggggaggtc
    25081 tttaccggga tcaacacccg atgtgccttt tcaccgaagc ggagtggaaa gccgctcttg
    25141 cctgggaagt caagaaacga atcggagaca gtccccggca cttcctggag aacggaaacc
    25201 cggatcagct cctgcattcc atcgaagagt cgtgggctgg gatgcaggtc ccattgggcc
    25261 cgctcaccca tcaaaccgtg caggggcaac tcatcgagct tttcaggccc gcggattccg
    25321 cgctctccgt ccccgatgcg gccctggggg aactcacccg gctgctaccc cgaaaagact
    25381 gcggagcctg cggggccccc ggatgtcgtg agttcgtcca acacctcgtt caaggcctcg
    25441 gtgaggctcg ccgatgcccc catctctccc cgaaacaatt gtccgaggtc gttcgccgtg
    25501 ccgccgagct ggacaaaagc tcttcccagg acggaagaca aaacccgaac ctgctggaat
    25561 tgctctatca ccgctctctc tggcgggggt ccgaggagaa acgcacgttt cagcaggtct
    25621 tgtctcccaa ggtccagcag tctcgccgac ggttcctgga acggctcgag gacctatgga
    25681 acaacctttc ccccaagccc cggatcttcc gggatctcga tgaggaaagg atttaccggg
    25741 acctttgcct ctacctgggg tacgaggccg tggaacggtt gggggaagag gagagagatc
    25801 tcctagtgca agggggagaa gaaaaacgcc gggccgagtg gcggcaactc aaggagcgcc
    25861 gcaactggct tgccctggcg gacgaaaaac ggctcagcag acccctcctc gcggctcggg
    25921 acgtgcaccg cacctcccgg gagacttacg attccgtttt gttcttgcac caattgcacc
    25981 ccgacgaccg ccggttggtc ttgcggcacc gggtgaacac cttcgaggac ggcttcagcc
    26041 gctggtggaa cgaggacctc ctcaccatga accttcccgg ctattccatt cgggactggg
    26101 aagacttttc caaaatcatt cataacgcct actggcacca ggagtcctcc gtccttcccg
    26161 gagaggcgct ggccgaactc gagaaggagg tcctgacacg cccggatctt tccttgcttg
    26221 tggagaaact ggtagggcga tggatcgacg aagaggcaag ccgcatcgct cgggcggcgg
    26281 acgacctgcg ctccttccgc aagggagccg aacggcgccc tatcggcaac gtccgggacc
    26341 tgaggcggat attcgaaggg ctgctgaacg aggccgaagc cctccggacc ctcgcaacgc
    26401 cttccgaatc ctcggggcaa ccgtcccggc ccggggggga agaccagaga aaacgcctgg
    26461 caatgaccct gtgggatcgg ttccaggaag aaggcttcct cttctccccg agcttttcct
    26521 tccactggga cgaactgcag ccggacgagc ggagagcgat ggagaacccg gcatggggcg
    26581 cggggaacgg cggaagcgct tcccctgcgg agggcgtgcc ccccgagaag ggaccggatg
    26641 gcgcccgaga gcgtgtatcc gtcgtgcatg ccgtcctcag taccgccgtg aaccggcgtt
    26701 gccgggaaca acgggaagcc gcctgggtgg aggaacggac cgcatcgacc ccggttcccc
    26761 tgggctccct cctcgtgtat gtcggccgtc gattgaggca gggacaaggt tcccgggagc
    26821 tgcaccggga gatcgtcgat ctgctcggca gcaattcatc ccttcgggaa agagtcttga
    26881 aggatgcctt gtttcgtttc atcgcgggac acctccggaa gtccttggag aatccggcgt
    26941 cggtcctccc cgtgtcttcg ttgcgggagg tcggaggaga ccgcttgccg gatccctggg
    27001 aagatctcga acccagcctt tcgcggatcc tcgacggaaa cccccccatg gaccgggaac
    27061 gactcttccg ttacctgttc gtcctcgcga agatggaggg caacctggat actcttacgg
    27121 ccttgcttcg cgagatccgg gagacctcgg acatcatcga ggccgcctgg ctcaaattca
    27181 ccgaagaacg catggtggaa ggaccaagcc ccaaggccat gccggggaca gccccgggaa
    27241 ttcccctgct ggcctcccga ctcaaagaca agggacccct caaccggggg ctccgggaag
    27301 gggtctcccg caaggaaaag aaggctgtgg cctccgctgt ccgcgaactg gagaacctgc
    27361 tccgatttca catcctcctc gcaacggacc acggaggaaa actcccggac ccggaagcgg
    27421 taatcgggga gatggagagt gcgggttacg acctcaccgg catcgaggag gaatccctcc
    27481 gcacggcggt ccgcaaggaa atcgaccgtc gggagcgctg ggcggctcac aagatctgga
    27541 tctacaccac cgcggtggcc cgtcgcctgg cagctcagca ttcgggtctc caggaggtgg
    27601 aacgggaact gcacaaacat cgcatggacc tgctcaagca cgaaccggga aggggcggcg
    27661 aattccaccg gatggccagt cttcgagggg tggccctggg acggatcaag gaggagttgt
    27721 accggcaact ctcggacctg ctcgaggcgg agcgcatcgc ctcttttcag aaacgcatcc
    27781 ggcagatcgt ggaacaactg gatcaaaagc gccgggagat ccgggaaggc tgggtgcggg
    27841 gagaaatcaa ccgcagaacg gtattctacc tgctgaggca gtaccagaaa ggtgaggaag
    27901 aacccacgtg ggaggacttt ctggtctttg ttcgagacca ctggctgctg ccgttggaga
    27961 gacttcgttc ctcggatcgc ccggatcggg aaaagcgtat ccgggacctg gatgcccggt
    28021 ttcaggcact cctgggggtc tccctccttg ccctcgaggc agagacgtcg gcccaggccg
    28081 atcgagacct ggaatcctgg atacaggacc ggtcagcgag ccttcaagga acgctctcca
    28141 tggggaaggg ttagaaaatg agcctatcat cgcaacctgc cgcaccgttc ggattcttga
    28201 cttcgaacag tccccccgga gactggatcg agcaacaccg tcaagcgcat cagcactacc
    28261 ggggtccccg ggacacccgc attcccccct ttctgggggg gctcgcccaa gccgtatcca
    28321 ctctgcgctt cagcctgatg caactccggc tggccctcga gagaacgccg tctcaaccga
    28381 cggatttcag acggcggctg ctcgattccg gcatgggcct ccatggagag gacccggagg
    28441 gacacctggt aaagctggtg gacaattacc tgtcccgcag ggactggagg gaacaggaca
    28501 tcctggagat gatcctggaa taccttgaaa ggtcctcacc tcgcgaggga gaactcgccg
    28561 gagaattggc ccggctgcgc tccctgcggg aacgagatcg cgaacggtat caacgagtgg
    28621 tcccgggaat gatgtccgag gcccggggaa acctcatggg attggggtat gcaagccgct
    28681 ttctgcaaga tttgaagccc cggtttccca agctcaatcg aggcgccctt gaagtcctca
    28741 ccggggcttt gcgcatgagc ctcgggcttc cccggggcgc catcgaggaa atcgcgaccc
    28801 gggaaacgcc tgccgatctc tggtcccggt tctacaagga ggccggggct cattggaccg
    28861 cacaagggac ctttcttacc ctcaagccgt tcgagaaggc ttccctctac ctcatggaat
    28921 ctttgcccat cgaaaaacgg gggcactcgg gcttcgtgcg atacctcctg gaaaacgagg
    28981 cggcctaccg acgcatcctg gcggcctgct acgcgtcccc ggaagaacag cagaatcgac
    29041 tcctcgagga aaccgaccgg tacaaccggt tgcccgaaca tgccctgcaa cccgcagcct
    29101 acgacgagga agagagggcc ctctttctgt acgccttctt tcttcggccc gacgtggtgg
    29161 aagccattgc ggagcgggag agacacaagg acctctacca ggccgttccg ggtctcccct
    29221 cccgagccct ccttccccgg gtgctccagg aagtgcagaa cgtcttcgga tacatcaccc
    29281 cggaatcctt tgaaaagatc gtggaaattc tcgagctccc tcccgtggag gtcatccgcg
    29341 tcatcgcttc ctacaagcaa tacagcgcgg accccggggg cgatattctg atctatgtct
    29401 gcaagggcac cgcgtgtttt cttcggggcc agccgaacct ctcccgcagc ctctcttcgc
    29461 acatccgggc ggagcaggga gaagtgggcg agcacggaat tcagttcatc gaaatggact
    29521 gtttcggggt atgccacctg gcgcccgtga tcaagtcccg ggacacgttt ctgggaaatc
    29581 gaaccgcccg ggacattccc cgcatgctcg atcaacttct caagggtccc gagtatgaca
    29641 accgtctcag ctttatccgg cgtattcagc aggagctggc tccgggccgg accagcgacc
    29701 ttcccgacac gatgcggatt ttgcgggtcg agggaaacga cggtgcaaaa tccccctctc
    29761 cgggcacggt cggagagggc cccattccct cggagaaaga cctgggcggc gccgggctcc
    29821 gaatcgactc cgacgggcgg gtccatgccg ttgccgcaga ggggagccgg gagctgggac
    29881 gggtgattcc gtcgtgtctc ccgttccact acaccggccg ggacgggtcc gtccaggtcg
    29941 gctgcgtggt gctggacgag ttcgggcagg caacggagtt catccatgtg gacgccgatt
    30001 tcctgggaga cgatctgcgt cgaaccatga agcccgcggt ccgggtaacc gagggaaagg
    30061 tttggatcga gcgcaacggt cctttcttgt gcctgggaga ctacatcgac caggccgttg
    30121 ccgtgaggtc cgaaaccggg gcggcgcggc tcgtggtgct gtccggtacc ccgggccgca
    30181 cccccccggc acgggaagaa ggagttgcca ggggatccgc cgatggggaa gacgcggggt
    30241 tcgctgcaag gcaagaccgg ctggtgttgg gatttgcggc cctcgcggac ccgcaggata
    30301 tcgattccta cctggccgtg ggcggatacg gggccgttcg aagggtcttg ggactcgagg
    30361 gcgaaccggc ctgggccccc cgggacattg tccgggaagt gagcgccgcc cggctccggg
    30421 gccggggtgg cgccgggttc ccgaccggga ccaagtggga ggccgtgcga ttggctcggt
    30481 gcgtcgtgga agagggcgac cacaaccggg atcccatcaa gttcattgtg gccaacgggg
    30541 acgagggaga tcccggggcc ttcatggatc ggaccctcat tcaggagcga ccccaccagg
    30601 tcctcgaggg aatgatcctg gccgcccttg ctgtgggtgc gcgctacggc gtcctctacg
    30661 ttcgcaaaga atatgaagac gccgttcgcc gactcgaaca cgccgtcttt caggctcgaa
    30721 ggaaaggctt cctgggtcga aacatcttcg ggacccccgg gctgtgtttc gacatcgaca
    30781 ttcgactggg tgcgggcgcc tttgtggccg gggagaagag agccattatg cgggccatcg
    30841 aaggggaacc cgccgagccc accctgaacg ccgtcagcaa caccttccga ggcctctggg
    30901 gaaagccgac cctgctaaac aacgtggaga ccctggccaa tgtgccgctg atcctccgga
    30961 agggcagcga atggtatgca agccggggga ccgaaaacag cggcggcagc aagatcttct
    31021 cggtcgccgg aatcgtcaac cggaccggcc tggtcgaggt tcgattcgga cgaactctcg
    31081 acgacattat ccgcatctgc ggaggggttc aggatggaaa gcgactggcc ggggtccaga
    31141 tcggcgggcc gtcgggggcg atcctgtccc tcaccggcgt gcgatcctac ttgctctaca
    31201 ctcccctcga cttcgacacc ttcgatcggg tgggagccat gctgggctcg ggaggacttg
    31261 ttttcatcgg ggatgacgac gacgtcgtcc ggttggcccg ccatttcacg gattggttgg
    31321 ccgaggaatc ctgcggacag tgtccgtcgt gcctgcaagg aaccgcgtcc ctgggctcca
    31381 ccctcgactc catcctggcg ggagaaggga ccgccgacca catccatgct ttgtgggcca
    31441 aaagcgacgc catcaaggca ggcagtcaat gcggcctggg gatgacggcg gccaacccgg
    31501 taaccagcgc ccttcgcttt ttcccgcaca gcttcctcta ccacgccctc gccaatcccc
    31561 ggttgaaccg gcaggagtgt ttcctcctcc tcgaggcgct ccgtctcctc acccgcgaag
    31621 acgtgcagcg cattaccggg cgccgcaggg aaaccctcgg ctactccttt accctcaaaa
    31681 agcacctggc tcgccacctg atgaccgagc tggaccggct ggaccaagta catcccggag
    31741 agccccggcg ggtgagacaa ctgttggacc tcctccaggt tccgccccac gagatgggaa
    31801 cccgggacat ttcccacgaa tgctccctcg aggagctcga agaccaccgg cgctatctcg
    31861 aggactgggc aggcgacccc gagccgccga gcgcctcccg ctgagcccca ctgggaggcc
    31921 gttgcgccaa ccgcacaaat atggagggca gccatgaatt catcccaaaa gcgatttttc
    31981 gattcattcc gagaagtggt gaaggccgtc agctccaccc tggacctcaa ccaggtaatg
    32041 gacctgctcg tgaagaacgt gaccgaggcc atggaactca aggcctgcgc cgttcgactt
    32101 ctcaatccca agagccggac cctcgatctg ctggcatcct gcggcctgag ccaagactac
    32161 attcggaagg ggcccgtgga tgcggaccga agcatcgcca gcgccgcgga aggcaacatc
    32221 gtcgtcgttc gcgatgccgg aaaagacccg agggcacagt accaacaaga ggccgtcgcc
    32281 gaggggattt ccaccatcgt gtccgttcct ctctcggtca agggaaggac catcggcgtg
    32341 ctcagaatct atactcacca accccgggac ttttctcagg aggaattgga cttcgcccag
    32401 gccctggccg aaatcggggc cattgccatc gagaacgcgc ggatgtatga gaggatcaag
    32461 aaggattacg agagcgtgat gagcgatctc tacacctttg tgggctaccg gcgaagcatt
    32521 tgatggccgg gaaaggacgg actccatgag caaccgacga gttcgtatcc tgatgggcag
    32581 gttcggggaa ggctatgatt cggccatgtt gcacctggcc cgggtgttca gtgaggcggg
    32641 cttcgaggtg atttacacgg aactgcagga cccccgggcc atcgtgactt ccgccatcca
    32701 ggaatccgtg gaccacatcg gcatcactac cctgcccggg gccgacatcg agctcttctc
    32761 ccagatcgtg gcccttttga aggaaatggg cgccgagcgc gtccgggtca cggcaggagg
    32821 gatactcccc gaggaggaca tccccagggt aaaggccatg ggggtggtcg agttctttcc
    32881 ccggggaacc acctatgggg agctcatcga ttggtgccgc aaaaacattc ggcccacgga
    32941 cagatgaccc cgggggcggt cctcgaatcg cccccgccca agatccctgc aatcgccgtc
    33001 gagctccgaa gctgctgccg agcattcgcc gtaaccgacc ggcgccgtgg taaggacgat
    33061 ttccgtagta cgaaggtatg gcgctggccc ttcgattgcc gggggaccgg agttaaggca
    33121 ccaagtttgt gctccgggtt ccatgcgtac atcagtcgtc ccccgggctc aggaacgggg
    33181 cgaccgatcc catggcccgg gccccggaag acccgcaaac gagcgtgcgg ctcgagctgc
    33241 tcataaaggg taaccttccc gtcagagatg ccgaaccgaa agcacgaccg gtgcagcaac
    33301 cccgcaaccg gccgagagtc gcggggcgaa gccgtgcctc atccctctcc cgcgggaaga
    33361 aacgcgggtc gggacgtcca cggcgaaaca accaagctcg tcgcagtcca cctgtggttc
    33421 tcatccgaag ttgcactcaa gccgaaactg cccacccgca ggattcggcc ctttgctcaa
    33481 tatttccact cagcatttcg gagcgacctt gggcgcaacc tggtatcagg acgctcccgc
    33541 tgcaaggcga tgggtcattt ggaagcaacc gccctttggt ttgacccact tcgacctcac
    33601 catcccggga ggatccgggg cggccgtcac ccggcgttgg gaaaaattcc ccggccccgg
    33661 cgtgttccca aacgctccca ccggggaggc ttcctgtcgc cgtatctttg gaaatccggg
    33721 gagttgcggg atattgatcc ccgacgggcc gggggcttcc gggccttttc cgccccgccc
    33781 tctcccgggc acccttcttt cctccaggcc ccggtatgcc tcttgctcct atatcggcgc
    33841 gacagattcc ggcgatccaa acgtttggat gcgaaatcgg aaacgccggc aacgatccgc
    33901 tcgaaaggag aacgaccatg agtgcttaca ggatcggaat ggacggaaat cttgcccatc
    33961 acgtgggaca cgctcccatg ggacctcact tccatggccc ccaatggttc ccccatgcac
    34021 agaccgatca ggtcccggag gtatgtccgt gctgtcgagg aagcagactg gtactggatt
    34081 cctacaacca gccccaacct tgccctcact gttttgagaa gaccctgcgc aggtaacccc
    34141 ttccccacgg cctccggccc ctcgggggcc gtggggtccg tcccgtggga aaatgcccga
    34201 atttcctctt ccgcatcctt tccataagat tccttttctc aacaaaggcc gcccttgttc
    34261 cggggaagct cacaaagtat ttgcattttg aaccccgtag tgttttttgt taggacaatc
    34321 ccacggagga accacagaaa tgggattcat gcccttggag aaaaaggaga agctgatgga
    34381 aaggcagtgg ttctacaaga atctccaaat ccgtgagggc ctcaagcccc aaagcgagct
    34441 cttcaaatac tacttcatcg tttccgacgg cggtcgaaag aagtgccgct actgcgtgtg
    34501 gattccggac gacgcattga cccagtacca ggaaaccggg gatttcgatg ccatcatcgg
    34561 tgctcacagg gaccgatgga gcctctgggt aacctccaag atcgacgcgg gggattttcg
    34621 aaacctggtg ctcaagctcg agaaatccgg cgaaaaggaa tacgacctgg attccatgga
    34681 cgaaaagctg accatggaat gaagcacgct tggcgcaact ggggcacggg caacggaatc
    34741 cgttcatact tccgacctca ttcctgccgg agcggttttg cgagggcctc cggacctcgt
    34801 tcccggtcgg ggaccggcgg ggaaatctgc ccgggcgacc tcgatcccgc gacccttcga
    34861 acacaggaag agaaagagac ggcgggcgag aacggggaac ggagcggcag gagcgttcct
    34921 ttcccgcagg agaacgtccg acctcacccg gagctctacg gagcgggcgg gatgattcca
    34981 ttgcggcacc gaccggcccc atggggagat tgccgaagag ggcagcgttg aggagggatc
    35041 gagcaaggtg agcattcgga ttttgattgc cgatgacgaa gagctggttc gcgagtcaat
    35101 cgcccaattc ctggagcatt tcggctacgc gacggatgtg gcggacagct gtgaatcggc
    35161 ctgggaccga ttgaggtccg aaaactacga cgtggttgtg acggacaaga acatgcccgg
    35221 atcggacggg ggccgtgaag gggggatgga actgctgagg ctggtcaaga ggcatcaacc
    35281 gggggccgaa gtgatcatgg tcaccgggta cggctccatc gataccgcca tcgaggccat
    35341 gaaaaacggt gccttcgatt acatcctcaa acccatttca cccgtttgtc tcaaggaaaa
    35401 aatcgagcgc atccgcgact ataagagcta cctgaacccg gaaaacacgg tgctcttcta
    35461 taaaaggatc cacaaccaga tcctggggct cctcgagggg cactcagccc tggcggacct
    35521 ggacagccac gaagtgctca aggaaatcga aacgaccctg gaccgcctct tcaccattca
    35581 gaaacagtac gagtccatcc tgcttctgca aaaggaggcc ctcgggagaa tcgccgcact
    35641 ctcggaagag ctccgggagg ccgtcgagac cctccccgag gtcgacgacg ccacggccct
    35701 gctggtccag cggatttgcg acgaagcatc cagaaggatc tgatgggacc cggccccgcg
    35761 acccgcggtg aggcgcccgg cagcaggcgg gtccccggta gggggcggag tcaacagacc
    35821 cgggacgacg gggtgaattc gtattctctc ccctgggcgt cgaaggtcac cttttcttcg
    35881 cggttctcct tgatcctgcc ctccaccatt cgcttgcatc cgaaggcgta ataggtgatc
    35941 tggtgggtgg gagcgccgga ttcctcgtcc ccggcggcaa gccggatgaa attgagcttc
    36001 tgacagatct cctttttctc cacgtctccg taacggatca ctacctgata gctcccatga
    36061 aacttgacgg gcattttcac gtccatggac ggctcctcgc aaggatggga taccggcttg
    36121 cgcccaaggt ccctcccaca tgttgagtga agatttcgag ccaagggctg aatcccgtgg
    36181 ggcgcggttt cggcctgagc gcaacctcgt atgtgagccg ttttcgaggg ttacgccagg
    36241 ttgtattcct tgagctttcg atagagggtc ttcctgccga tgcccaggat ttccgccgcc
    36301 tgggagcggt tgtgattcac ctgttcgagc accttgagga tgtgctgtcg ttccacgtcg
    36361 tcgaggctca cgatctcggg tccttctcgt tcttccacct tttgttccgt gagttctttg
    36421 ggaagggcct tgggggtaat ccatccgttt tccgaaagaa tgatcccgcg ctcgaccacg
    36481 ttgcgaagct cgcgaacatt gccgggccag tggtaggcca tgaggtactg catcacttcc
    36541 ttggaaaccc ggcagggaga ccttcccgtg cagagcagtt ccaggtagaa gtccaccagg
    36601 atgggaatgt cctccttgcg ttcccggagc ggcagcaggt cgatgtggaa cacgttgatc
    36661 cggtggaaga gggcttcgtg gaagcgtccc atcttcacct cctcggccag gtttcggttg
    36721 gtggcgaaga tgaaccggac atcgacctgg cgttcctgct gatcgcccac ccggcgaaag
    36781 gtctgcttct cgaggacccg gagcatggat gcctgcacct cgagagggag ctccccgatc
    36841 tcgtccagga agaacgtgcc cagatgcgcc agggccagca gaccctcctg ggaagccgtg
    36901 gcgccggtga aagcgccctt gcagtagccg aacagctcgc ttcgaaccag ctccttctgg
    36961 agattgccgc agttcttgat gatgaagggc tggtcggccc gggcgctgcg gttgtggatg
    37021 gcatgggcca cgacgtcctt tcccgtgccg ctttccccgg taatcagcac ggggacgttg
    37081 gtgggggcca ccttatcgat gaggtaatgg atctgctgga ccacctggga ctcgccgatg
    37141 agatgggaag gcgggacatt tcgctgggaa tggcgaagga gacgattttc ccgctggagg
    37201 cagaccctct gaaaggcctt ttccaccacg acctcgaggc ggtccaggtc aaagggcttg
    37261 gtgatgtagt cgtaggcccc ccgtttcatg gcctcgacgg cgttgtcaat atttccatgc
    37321 cccgtgatga ggatcacctc cgcgtccgga gaggcctctc gcagccgatc cagcaggtcc
    37381 agcccgtcgg cgtcggggag cttgaggtcc agcaggatca cgtccacctg gtgccttcgg
    37441 atcaggtcga aagccttctg tccggtgtcg gcggtccgga ccgtccggcg gggcccgccc
    37501 aactcccgct gcacaagtcg gcggatggac tcttcgtcgt ccaccaccaa gagctgtatt
    37561 ggattgattc ggctagtctg catctttcat ctggcgcggt aagcgaacca agaacgtgga
    37621 gccctgccct tccttgctgg taacgacgat ttctcccccg tgcttggtca caatgttgta
    37681 acaagtggaa agcccgatcc cgatcccctt ccccacgggc ttggtggtga agaagggctc
    37741 gaacagcttg tcccgatgtt ccggaggaat gccgcacccc gtatcttcca cggcaagccc
    37801 cacccagttt tcgttttcga ggaaagtgcg aagggtgatg gtcccttgga ccgcgatggc
    37861 gtccagggcg ttgaacatca ggttcaggat cacctgcttc agttggggct cgtctccgta
    37921 gacgggcggc agcagctcct ggaggtccag gcagaccacc ttttggggaa agcgtttcag
    37981 atggaactgg agcagcttga gcgtctccgt cacgatggcg ttgaggttca cgggaacaaa
    38041 gtcgtgggaa gtctggtgcc cgaaggtgag gaggctcctc acgatttccc cgcaccgttt
    38101 gcattccttc aaaatgatcc ccacgtactc cttgaagtcc tcgattagcg cctcttgccc
    38161 cggttggcag agcctgctca atcggcgttt gagcccctcg gcaaagccgc tgatggccgt
    38221 gagggggttg ttgatctcgt gggcgacccc cgaggccagg accccgatgg tggccatctt
    38281 ctccgcctgg tagtacttgg cctggtattc cttctccaac gtcacatccc ggatgagaag
    38341 caggatgaaa gcgggttctc ccagggaatt gcgcaagggc gatgcggtca cctcgaaatg
    38401 gcggttcctg ccttccacgg agtagatgtg gtttttccga caaacctcgt tggtcttcgc
    38461 ggcggtgacg gcgggacaaa ccgcgcacgg ccgatccagc tggtggaaga cctggtggca
    38521 gaatttcccc tcgggcttgg gatcgggata ggtttcgtaa tacacgtgat tgaccgattg
    38581 gatcctcagg tccttggaaa gcaccaccat gacgtcggtg atcccgttca ggatcgcctg
    38641 gatctgctgt ttcttctggg ccagctcctg gttggacgcc ctcaactcgt cgatggtttc
    38701 ccgagcctcc cggaagaacc ccaacttcac gtattcgatc ccgatcacgt cctccagggt
    38761 gggcttcctt ttctccatgt caccaggcct ccttgcagat ccccaggaga tcctcccatc
    38821 cggcctcccg agggtttgtc agaaggcata catccccggc ggcaatccgg cacaaatcgt
    38881 ccaacaggga tggatcggac acgatttgcc tgaggttcaa cgcccctccc agggatgcgc
    38941 aaaactcgtc caacttgtcg atggcggcgt cacaggtagc ctggtgggat ccgtagcgtt
    39001 ttcccaagat cgtgtcgccg ataacggcca ttttgtttag gcatttcgaa gaattgaagc
    39061 gaaggacggc gggaagcagc acggggtgca cccacccatg gagcacatca aacattccgc
    39121 ccaaggcatg ggccaatgcg tgctcggctc ccacccccgc attgctgaac gccatggccg
    39181 cggcattgga cgcaatgctc agctgttcga ggtactcgag ctccctggat tccacggccc
    39241 tgcgggcaaa tttcatgatc agctggatgg cctggaggga ctgagattcg gtgaagggag
    39301 aggcaatccg ggaaacgtag gattccaccg cgtggctcaa ggcgtccagg gccgctgcgg
    39361 cgatgagatc ctcgctctgg gtgaggagca acgtgggatc cacgatggag atgttgggca
    39421 gaagcgtccg gctgatgatg gacatcttca cccgtcgttc cacgtcccga atgacggcaa
    39481 actgggatat gtcggacccg cttcccgccg tggtggggat cagcagcatg ggaggcaggg
    39541 gataggaaat acgattggcc ccttcatagt cccggatctg gcccccgttg ctggccacca
    39601 gggcgatgcc cttggcggtg tccatggggc ttcccccccc caacccgatc accacgtccg
    39661 cctcctgctc ccggtagagg ctcaccccct cctggacctg ggaatcccgg ggattggtgc
    39721 tcacgttgga gtaataaacc cagcggagct tttccttttt gaggagttca acgacccttt
    39781 cgacccaacc cgccttttcc acccccgggt cgctcaccag gaataccttc tcggccccca
    39841 gctggcgcgc gcacacgccc acatactgga cactgtcacg cccgaagatg atctcgggga
    39901 tcgagaactt caagacatgc atgaaggccg cctttcaggg aaagggaact ccggacgagt
    39961 cacgggagca ctatgacaac gggttgagct tcgatcacgg accctccgag ggaaacggaa
    40021 tcagcttacc cgaaccggca caaataagca agttttggcc aacaacgacc ggggaacttc
    40081 caagggggat actcccggag cgtccgtgtg cctgcagccc tcaccccggc cccagtttac
    40141 cgagtgaccc gccgaggatc ccaccgtcat ccctcccccg acctccggcc tcacaccctt
    40201 ccatgcctcc ggtgaaccaa ccagccgtgg cgggcattgt aaaacggcgc ccaccctacc
    40261 atagccccgg gtgggcacgg gtctcgtcgt gcccatcccc ttggccttgg agagagggaa
    40321 acatccccgc cgagacaccc cggccgaacc cggcggcggg tcccccgggc agggcggtcc
    40381 ttcgacaccc cgcctgcggg gcgtctccct cacgggcgac gggacgacgg attcttagat
    40441 ggttggatga gaggagaccc cgcgtctccc t
//
LOCUS       DAOOVC010000019        39804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_653_length_39804_cov_6.403859, whole genome shotgun sequence.
ACCESSION   DAOOVC010000019 DAOOVC010000000
VERSION     DAOOVC010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 39804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39804
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_39804_cov_6.403859"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PLM79_04375"
     CDS             complement(<1..848)
                     /locus_tag="PLM79_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HOV85573.1"
                     /translation="MGAVQKTYQEINEKIRKGKAVVVTAEEMIGIVKKKGPARAAREV
                     DVVTTGTFAPMCSSGAFINFGHTKPPIKAARVWLNDVPTYAGLAAVDLYIGATEPAFD
                     DPLNKIAPGHFEYGGGHVLHDLVDGKPVRLRAEAYGTDCYPNRKVEMTVTLKDLPYAV
                     LCNPRNGYQNYNVAVNLSQKTIYTYMGTLKPRMGNANYSTSGQLSPLLNDPFYRTVGL
                     GTRIFLGGAQGFVVWPGSQHNPHVPRSENGVPRAPAGTLMVMGDLKQMSARWIKGVSL
                     LGYGCSL"
     gene            complement(1091..1549)
                     /locus_tag="PLM79_04380"
     CDS             complement(1091..1549)
                     /locus_tag="PLM79_04380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOV85574.1"
                     /translation="MTDARTVSVPRSLEISIRVRFGDTDPYGVVYFVSYFRYFHRGIE
                     EFLRSRGLAPEKTFRNPEEGYGLPIAGASCDFYRPVWYGAELRLLVFVEKIKPKSLTF
                     GFQFYPADGRDLIAQGRATMVAIDRTWKSRVVPEGLLKVLEADPATPSNS"
     gene            1760..2233
                     /locus_tag="PLM79_04385"
     CDS             1760..2233
                     /locus_tag="PLM79_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV85575.1"
                     /translation="MPAEPERALSLYEDGIHRALSLDLEGASRRFEEVIALDPRHVGA
                     RCNLGAILRLQGRTDEALGNFQQALRIDAGHARTLSNLGAVFHDQGRIQDAELAFCRA
                     IRLDPTLGEAHYNLSLLYCRRQQFDRAWNHAREAAGLGMREGAVLLEEIERLMER"
     gene            3410..4435
                     /locus_tag="PLM79_04390"
     CDS             3410..4435
                     /locus_tag="PLM79_04390"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOV85576.1"
                     /translation="MDIDKRMTLKDAVAKFIHDGDTVYYGGFQIAVPMALTHEIIRQK
                     KRHLTTIESSTDVGGLDLLVGAGCVSEMHTAWVMNWYVKAPYCIRRAFNKGHLKRYDI
                     SNFGATSAMMAGFLGIPFIPIRGNTGTDMVKHNLMDLRTIQDPFTGQNTTVVRAWRAD
                     VAIIHAQKADRLGNVVSYGTRGVTDEFGAMGAKRGVVVTAEEIVEPEVIRSDPDRVIV
                     PYYKTLAVVHCPWGAHPSASRGYYGLDIRFCQYQGRYERHAELLPYFLDEWIYGCEDH
                     AAYMKKYVDTFGQEALDRLKPILGFKPKLDVDYGYHDPAVWKGVPMYTDDRLKAYEEA
                     LAKKGGK"
     gene            4438..5205
                     /locus_tag="PLM79_04395"
     CDS             4438..5205
                     /locus_tag="PLM79_04395"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOV85577.1"
                     /translation="MADYKLTELMTIRCAKEIKDGEVVFVGVGLPIVVAAFAFATHAP
                     NMIMFTEGGAIRATPPRGLAITVDDPALFTEADATFGMLATMAALQRGEVDVAFIGGA
                     QIDKFGNINSTGAGDWSKKESFTYFAGSGGANDMATSGKRVLAIMPQDRKKFVERCEY
                     ITTPGYLDGPGARERLGMIGGGPDCVVSTMGVYRFDEDTKEMYLAEYFPGQSVEKIKA
                     NCAWDLKVSPSVIETTPPTEEEVKILRAIDPTGFYLG"
     gene            5512..6078
                     /locus_tag="PLM79_04400"
     CDS             5512..6078
                     /locus_tag="PLM79_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85578.1"
                     /translation="MEPKILAAGHKSGGRNKLEERYLKYRHYYATIDICSSGEYGKAV
                     YAPLLPAFDQWYKRKSLSDVPPNIPFLEDRLYRTDHPDFREERGGTISFHIKVLALSM
                     IGLIMKYNRKVITDQILNYILFEVSQQTKSYNYSECLCFMITVQMFIEMLKEDFSHFG
                     SQVFDLTLSDFANEETLNPKPQPVISIL"
     gene            6106..7005
                     /locus_tag="PLM79_04405"
     CDS             6106..7005
                     /locus_tag="PLM79_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOV85579.1"
                     /translation="MEETLKGDNARDPDPRESLAERHLEELACLVDELGDSQLVIQDH
                     DLLEWAKNHGISPRDAVVEVLRSRIVPLRYAGNVRALSLAEQRRVCESRVLVCGCGGV
                     GGVLINLLARAGVGTLRLVDGAVFVPSHLNRQWHCDTRELSRPKAEAAGERARAANPL
                     IKVEVFPVAMDEKNAESLVEGTDLVMDALDDLPARFVLAETAKSKKVPLIHTAAVGWW
                     GQVSTFLPESIFDLRNVYGRGEEQSQSDEWMGALGPAPTVIGGLAAFEALRLLAGRNP
                     AYADQLCYLDGESGRLDVIYLEP"
     gene            complement(7017..7646)
                     /locus_tag="PLM79_04410"
     CDS             complement(7017..7646)
                     /locus_tag="PLM79_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85580.1"
                     /translation="MFVKQMQVGPMAVFAYLVGDPETGEGLVIDPAARVDDILRQARE
                     NHLEIQYIVNTHGHVDHMTGNAEMKQKTGARLVVHEQDAELVMFTPLPLYRMHGAEKS
                     PYPDLLVKDGDDIVVGSISLRVLHTPGHTLGSICLYTPGYVFTGDTLFVGSAGRTDFP
                     GGSRAMLRRSLLEKLFNLPDDTVVMPGHNYGSTLSSTIGHEKLHNPYLK"
     gene            7955..9322
                     /gene="bioA"
                     /locus_tag="PLM79_04415"
     CDS             7955..9322
                     /gene="bioA"
                     /locus_tag="PLM79_04415"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HOV85581.1"
                     /translation="MDPISPRTRSLEEDDKKYLWHPFTQMRGWMQEETLIIEEGDGCW
                     LKDVDGRRYLDGVSSLWTNVHGHRKEPLDRALKDQVDKIAHSTLLGLANIPAIQLAKE
                     LIEIAPPGLTRVFYSDSGSTAVEIALKMAFQYHQQCPRGNPSKTRYVCLSNSYHGDTI
                     GSVSVGGIDLFHSMYRKLLFPAYRAPSPFCYRCSLGKSPGSCSMDCLLEMEKILQQHA
                     PEIAAVIVEPLVQGAAGILVHPRGYLREVRGLCTRYGVFMIADEVAVGFGKTGTMFAC
                     EQEGVTPDLMALAKGLSGGYLPLAATLATEEIYEGFLGEYEEFKTFFHGHTFTGNPLG
                     CAVALAGLEVFRRENVLDSLQGKISHLARNLDAFRSLSHVGDVRQCGIMVGIELVADK
                     ATKEPYPFGQRMGHRVILEARKRGVIIRPLGDVVVLMPPLAVSLEEIDTLCAVVYDSV
                     RAVTE"
     gene            9340..9633
                     /locus_tag="PLM79_04420"
     CDS             9340..9633
                     /locus_tag="PLM79_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85582.1"
                     /translation="MKEEIRTGSLRFDPTDPIYAEHFPDRPVVPGSLVVGAFLEIAEG
                     MGFLGSEVAVENFRFREFVVPGLYDYRVEARGDTLRCFLSSGDKKLVTGSLKR"
     gene            9636..10376
                     /locus_tag="PLM79_04425"
     CDS             9636..10376
                     /locus_tag="PLM79_04425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV85583.1"
                     /translation="MEYPGTLALILGGSCELALHLARAMIGDALRPILTYRNEAGRDR
                     IVRGLEGLSGRYESRRLDLADRTTLEALLDSWGEDPLDFTVDFAHGDYEELVAAADDD
                     VLERYFRENVSSRACFLRRAARIMLRRRKGRMVFVSSGAAENPNPGQGFYAASKLASE
                     ALYRSVGLELGRRGVTTVILRPGYVRAGRGRAYLEREGKQVSRKIPTGKALEGEEVAR
                     TILFLLSTHARNLNATVVTMDGGLSSGK"
     gene            10391..10837
                     /locus_tag="PLM79_04430"
     CDS             10391..10837
                     /locus_tag="PLM79_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOV85584.1"
                     /translation="MELFESVQGILTEILDLEDAELTPETYLIRDLGAESIDLLEIAV
                     AMSYRFKIRVEDDEAFLKNFRLHLVEAQQEDLQPADYLAERYPFLSRDRIEEILSDLE
                     GGPTVKIKDLVCYAAHRLDGAPADESDPLLPLLSAQVGVRAVAGPR"
     gene            10845..12077
                     /locus_tag="PLM79_04435"
     CDS             10845..12077
                     /locus_tag="PLM79_04435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOV85585.1"
                     /translation="MSGKRRVVISSSGVISSLGDTPGRILESLKEGRVGFRPSSADPQ
                     VVVCPVEGFRPKSFLGPCKDSRYLHRGALFAAAAAMIAVRECGIAGEERSGAGLFVGV
                     GPNLDIGGEFPSVAEGRLDRPDLPALWILRFLPNTAASVISKLAQIHGENLTVTTACA
                     ASLQAIGEGFRRVREGHLDLAFAGGGDSRLSPGGILAYRKAQALCIGAFPPDLAMRPF
                     DADRAGFVPGEGGAFFLLEELEHAKRRNAPILAEVCGYGSSLDGYSMTAPDREGKGAR
                     RAVETALREARVRPSEIDVVFAHGTSTPLNDEVEARLIEELYPSGSPPVTALKSWIGH
                     IASACGALELGIALCCMREGYLPGIRNLRAPCNPRVRFLDSDVNLSCRTAILENFGFG
                     GQNAALVIRRYESHARGE"
     gene            12083..12535
                     /locus_tag="PLM79_04440"
     CDS             12083..12535
                     /locus_tag="PLM79_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85586.1"
                     /translation="MENPLVIRSMQTGFLLLDRILEVGAGKIVGEKTFHGGPAYLGIE
                     ALAQLGAYHVRFLNDFRKHAFLLKIQSCTIPTGSTLRGPYRLFGELRAGSAHSYAHHL
                     EARREDGIVWGGEFLFSTVEYDDRFKKDLLQTHYGKIFSCLRSAMKTG"
     gene            12505..13659
                     /locus_tag="PLM79_04445"
     CDS             12505..13659
                     /locus_tag="PLM79_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOV85587.1"
                     /translation="MFAKRYENRLNLQKNAGLYRNPPEFDRREDRFLLLGGKKVLNFA
                     SNDYLGLSTTEIVGTQVARNFRKFGSSSSSSRLVSGNYSVINRAEEAYASYFGYEAAV
                     FFPSGYQANLGILSAFFEKGDTIIFDKHIHASSVKGMTLSGAAFYGYNHNSMDHLAKR
                     LEGSGHPQVAVLTESLFSMDGDLLNVAGLRRLKEKYGFLTVVDEAHSFGALGPRGKGI
                     AREVADVALGTFGKAFGLFGAFVLLPRLFKDYLFNFCSPLIYTTTLPEAHAASALDLL
                     EIVEKSEDRRSHLADMSRLMRRRLREQGFKVSGDAHILALEIGDEDKAVRVSRKLLEN
                     GIYALPARYPTVPLKQAILRIGITALHREDDIRLFVDTLRRICDELESRP"
     gene            13637..14341
                     /gene="bioD"
                     /locus_tag="PLM79_04450"
     CDS             13637..14341
                     /gene="bioD"
                     /locus_tag="PLM79_04450"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HOV85588.1"
                     /translation="MSSNQDPEILSVVGTDTGVGKTVLSLLLMQFFFAKGLKPFYLKP
                     VQTGCRDPYDTDSDARFVYEHVEALRGRDPAESVIYCFREPKAPYFAARNEGSTVDPR
                     VIRKVVEEKRLHHSPLVLEAAGGILVPMTSTQHGIDLAAETGARPVLAARSALGTINH
                     TLLTLEALRARNLDPLGIVFIDPFDPSTPRDLVEENMEAVRDFGGISVAGVIPRIPDF
                     SNPPGECFQVLGRLLA"
     gene            14612..15022
                     /locus_tag="PLM79_04455"
     CDS             14612..15022
                     /locus_tag="PLM79_04455"
                     /inference="COORDINATES: protein motif:HMM:NF039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5663 domain-containing protein"
                     /protein_id="HOV85589.1"
                     /translation="MPLKPASDEPPRQSPSAPVRNPYILNFCKVLVEKKGEQHPPDAL
                     KKLLHDMYRLFEAMLGQNMVRALPEDLRRKYLTLCEDLSRLNYETIGDIFDKNVPQYE
                     QVMKSTMKQFAEIFMRNRVFNPKDYPVPLKDDSE"
     gene            15043..15327
                     /locus_tag="PLM79_04460"
     CDS             15043..15327
                     /locus_tag="PLM79_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HOV85590.1"
                     /translation="MPEPEDKTICPHCGVKMLKWKTPMLSTWSAEFFYVCFNDDCPYF
                     VRGWNHMEKTMQNTCSYRHRYDPTTGACGPLPVWSVDATRSRIVDDSEEG"
     gene            complement(15656..16447)
                     /locus_tag="PLM79_04465"
     CDS             complement(15656..16447)
                     /locus_tag="PLM79_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV85591.1"
                     /translation="MNMSLEKTAPMTIQDRLEPLSEGRFRFACHPKVPCFTECCRDLR
                     LQLTPYDIIRMKNQLGMTSGDFLEEYAEIRPEEQGTLPMAYLRMANNERKTCPFVSPP
                     GCRIYEGRPSACRIYPVARASRVHPTHDAVQEHYYLLREEHCRGFSEPREWNVRDWIS
                     DQGLDSYHEMNNPWMEIVTHPAIRRAQGFTLGQQQMFFLAAYDLDRFRSFVLGGRFLQ
                     LFDLPEEEIEAIRVGDESLLRLAFRWLSFTFLKKPVLNVRRTGSP"
     gene            complement(16484..17206)
                     /locus_tag="PLM79_04470"
     CDS             complement(16484..17206)
                     /locus_tag="PLM79_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV85592.1"
                     /translation="MNYSLDEKSTGTLQSQSPFPFQCRKDLPCFTQCCRDVNIFLTPY
                     DVLRLRRSLGLSARELLERHTHHYLAGPSHIPVVQLLMDPRTLSCTLVTDQGCRVYQD
                     RPWACRMYPLDLTPRQGEYRLIVGKDRCLGLRERAVRSAGEWLEGQGVKEYLEMEEAF
                     QRVIPPGTEPGRTMEPGLGRLLFLAYDLDRFAGLLGDEKLQRFYELDPSTLEAVLRDD
                     EALLRLAFRYIRSQMEELSALL"
     gene            complement(17222..17482)
                     /locus_tag="PLM79_04475"
     CDS             complement(17222..17482)
                     /locus_tag="PLM79_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85593.1"
                     /translation="MTSKQDAENVVGRDFFLAKWEEDQLTMEPFCSCGQALDEDYFCP
                     KCQRQCTIHYFVCEDSTTLSVVRRFIQGNPSFRNFEADLLKQ"
     gene            complement(17551..18711)
                     /locus_tag="PLM79_04480"
     CDS             complement(17551..18711)
                     /locus_tag="PLM79_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85594.1"
                     /translation="MEPRIISLEMRDYIAEMGAETVNWCMQCGLCTNLCPWRLVPGET
                     SAKFNIRHMQRLGQLGMEGFEEEEILFACSTCGMCQSNCPRGVKIVDNVRAMRAGIVG
                     AGMAPAHLRPILGSTHANGNPWAGPRQKRTEWQGKMEVPNFTPDMEYFLFVCCHSCYD
                     PRSTRIARSIVRLLQQAGVSFGVIGIEESCCGETIHKMGDEELFQKLAHSNIRLFNER
                     GVRKIVTTSPHCLWTFKNEYPQWGGEWEVSHYTQLLSRLVKEGRLSFPGEFARKIAFH
                     DPCYLGRQSGIFEDPRDLLGSVAGVESLELGRSGPLSLCCAGGGGRIWGEVPMGERFG
                     ELRITDAVELGAQVLTTACPYCVNMLTDACTSLGKQESLEILELSEVLAKAL"
     gene            complement(18815..21034)
                     /locus_tag="PLM79_04485"
     CDS             complement(18815..21034)
                     /locus_tag="PLM79_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85595.1"
                     /translation="MEKKPGVYICAGCGIGSAMDVQALTKVAVGEFKVPVCQTHECLC
                     GKEGLALLHAHRSESGINTFVLAACSQRVMTDVFDFGPDCIVERVNLREQVAWCHRPG
                     EEDTLMMAEDQLRMGIIRARETELPAPFCIEGPSRKVLVVGGGLAGITAAREAAKAGY
                     ETVLVEKTDRLGGYLKGVHKLPPLGPPYEEPEEFNLEELVRELEEDSRVTVYTGARVG
                     RISGAPCMFDVQILQNGSEIRERVGAVVLATGSVPYDPQRLEGLGYGTYPDVITASEL
                     ESMFQNGGPVRPSNGQPVKSVAFVLCAGSRDPRHLPYCSAACCVESLKQAKYFKAADP
                     AVPVYVFYRDLRAHGTYELFYKELQKDGVLFVRGSVTSVSDDGSGGLVVQSDDVLTGS
                     PVLSESIDLVVLATGMVPTTAFGEPFKAQTPKEGEEESSVPEDAVLISNLLNLEYRQG
                     PELPALKYGFPDSHFICFPYESRRTGIYAAGTVRAPMDYARAEVDGTGAALKAIQCVE
                     MVAEGKAVHPRAGDLTYPEFFMQRCTQCKRCTEECPFGAINEDEKANPLPNPTRCRRC
                     GVCMGACPERIVSFKNYSVAMVGNMIKSMEVPEEDEEKPRILVLVCENDAYPALDMAG
                     IKRLSYNPWVRFIPVRCLGSINLVWIADALSKGIDGILLLGCKHGENYQCHFIKGSEL
                     AEIRMSKIQETLNRLVLESDRVRLEQISVADYPRLPGILDEFAARVQELGANPYKGF"
     gene            complement(21039..22307)
                     /locus_tag="PLM79_04490"
     CDS             complement(21039..22307)
                     /locus_tag="PLM79_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV85596.1"
                     /translation="MAEAANGSRSILIAGAGMTGLSAAVEAAEAGFQVILVEREAYLG
                     GRVARMNRYFPKLCPPGCGLEINFRRIKNNPRIRFLTMAEVEKIEGEAGNFEVTVRVR
                     PRFVNENCTACGACEAAARSTFPNPFNYGLDRVKAIHLPHEFAYPMRYVLSPEVVGTR
                     EAEEIRDACPYGAVDLDMQVREVPLKVGAVLWATGWNPYDAGKIPYYGYGKHKNVVTN
                     VIMERLASPGGPTGGRIVRPSDGGEVRKIAFIQCAGSRDENHLPYCSGVCCLASLKQA
                     TYLLQQNPQAEVVIFYIDIRAPGKYEDFFAAVQAHERVRLIKGKAGELTEDPPGSRVV
                     VRCEDQSTGEILKEAFDLVVLATGMVPSTATEKIPATLTYDDHGFVLSRQGTPGIIGA
                     GCVQHPVDVASCVQDATAAALKAIQALGRR"
     gene            complement(22779..23078)
                     /locus_tag="PLM79_04495"
     CDS             complement(22779..23078)
                     /locus_tag="PLM79_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85597.1"
                     /translation="MADGKYPINLWVNEDRFAKLQAAGLASMTKDAMAGLKVIYVYAN
                     DAQKDKILKVFPTAKFDSATTKSIELLPRKVKDKIFDLVIEKKSIDVLDDFLKDY"
     gene            complement(23092..24351)
                     /gene="sat"
                     /locus_tag="PLM79_04500"
     CDS             complement(23092..24351)
                     /gene="sat"
                     /locus_tag="PLM79_04500"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HOV85598.1"
                     /translation="MAVIGHGKKEIVEKIMAPDEAVKKIQGLTQVPVRSQIANEIIGI
                     SYGFFSPLEGFMGKADVDGVVQNMHLANGILWSIPIVFDMSDADIAKYGIKEGQSVVL
                     TYDGNPMALFEINEIFTYDKKVMAEKVYGTTEEKHPGCARTYKYEEKFIGGKITLVNK
                     PKINEPYGPYFIPPIQMREEFKKRGWKRVVAHQTRNVPHTGHEWLMKGAWSQTYGELP
                     IDKPLVGVLVNAIIGEKRKGDYIDEAIILTQDELRKSGYFGDHNHLTSLTFWDMRYAG
                     PREAIFHAILRTNLGLTHHMFGRDHAGVGTYYGAYDAHNLLAKYQDELDIVPVYSMNW
                     LYCPHCGEVTSEGLCNHKSEWQKFSGTLIRSIVDDGVKPPRLIFRPEVFDLVMECAEK
                     YGFGSPFVTDEYLAKRTPVFSIEPLKA"
     gene            complement(24520..26433)
                     /gene="aprA"
                     /locus_tag="PLM79_04505"
     CDS             complement(24520..26433)
                     /gene="aprA"
                     /locus_tag="PLM79_04505"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HOV85599.1"
                     /translation="MANFETVVVDTDLLIIGGGMSACGATFEAAYWAKKNNLKVVVVD
                     KAAMDRSGAVAMGLSAINQYINYAKGVNTLEDYVRYVRQDLMGISREDLVYDIARHVD
                     STVHLFEKWGLPIWKDEQGNYVNEGRWQIMINGESYKVIVAEAAKNAMNQAGFTDWLY
                     ERVFIVEPLMDFDNNKCLGAVGFSVRENKFYVFKAKATIAMMGGAVHVFRPRSVGEGL
                     GRAWYPPWNAGSTAYFTIRAGAEMTCQEVRFIPVRFKDGYGPVGAWFLLFKSRATNSL
                     GEEYMVTRKDELPKWAPYGEGKPMPANLRNWLGMEDVMAGKGPIFMRTEEAIANLAAK
                     YKDDPKAFKKKMKELEAEAWEDFLDMTISQSLLWAGMNIQPEEKSSEIAAAEPYFIGS
                     HSGASGAWVCGPEDVMPAEYRAQWEPIGVYNHMTTVKGLFTAGDGAGASSHKFSSGSH
                     AEGRIAAKGAIAYILDNNTAPQVSDAVIESMKKTILAPLDRFEEFAKLSSDPDINPNF
                     IKPKMFMFRLQKIMDEYCGGVSAQFTTNKALLERGLELLQMLKEDAEKLAASDLHELM
                     RAWENTHRMWIAESHLRHVLFRQESRWPGYYFRADLPDMDEANWRVFVNSVWNPKTGE
                     WTMKKVPVVNLPV"
     gene            complement(26451..26894)
                     /gene="aprB"
                     /locus_tag="PLM79_04510"
     CDS             complement(26451..26894)
                     /gene="aprB"
                     /locus_tag="PLM79_04510"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009973 - adenylyl-sulfate reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA];
                     GO_process: GO:0019424 - sulfide oxidation, using siroheme
                     sulfite reductase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit beta"
                     /protein_id="HOV85600.1"
                     /translation="MPSYVILEKCDGCKGQDKTACMYICPNDLMVLDKEKMKAWNRAP
                     EMCWECYNCVKICPQQAIDVRAYADFVPMGASVVPLRGSEDIMWTVKFRNGQVKRFKF
                     PIRTTPEGQANPLGGYETGTDDIKSPILCTEPASTGQSTLPTLKK"
     gene            complement(27333..27554)
                     /locus_tag="PLM79_04515"
     CDS             complement(27333..27554)
                     /locus_tag="PLM79_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85601.1"
                     /translation="MEKAVFGSFGAVVKVTGRFLFLEEGKLGGLGAQKRKKKGFGGRG
                     SLPGALLGASMGIRTDFASEIVKRTSFFP"
     gene            27837..28886
                     /locus_tag="PLM79_04520"
     CDS             27837..28886
                     /locus_tag="PLM79_04520"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOV85602.1"
                     /translation="MESPWLSTTGRHILALILLSGLLIGFGTAGYILIEEFSLLDGLY
                     MTIITLTTVGFGEVKPLQPMGRVFTILLILMGAGLVAYNLAYFSQLVLDGNLLVLYRR
                     RRLRKQLEHVADHYIICGYGQMGKIIAQELSKNHVPVVVVENDPSAVVSIREKEIHYF
                     AGDATEEETLLSAGILRARGLISVVSRDTDSVFIIITAKDLNKDLTIFARATTPGAER
                     KLLKAGATKVVSPYASGAIRIAHNILRPTVTDFLELALSAEGMELSMEEIRLPSEADL
                     VGMELMYSGIRSRYNLIVVCIKRSSGAMIYNPSPQEVLKEDDILVVIGPQENLNRFSA
                     DLFGRPRPSQRPGRS"
     gene            29198..29989
                     /locus_tag="PLM79_04525"
     CDS             29198..29989
                     /locus_tag="PLM79_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOV85603.1"
                     /translation="MEYANVLFEIRDGVGILTFNRPKALNALNVETLEELNDVLDRVR
                     EDDAVRVLILTGSGEKAFVAGADISEFPRMNALQAHRFAERGQEIFSKLEELPKPVIA
                     CVNGFALGGGCELAMSCDFIYASSLARFGQPEINLGLIPGFGGTQRLPRLIGRAKAKE
                     LCMTGEMIDAQQAMELGLAARVFPPEQLMEETLKVARLLASRSPVALRALKHAIDRGW
                     DLDLRNGCSLEAESFGLCFSSQDMREGVAAFLEKRKPNFQGSLTR"
     gene            complement(30792..30986)
                     /gene="rpsU"
                     /locus_tag="PLM79_04530"
     CDS             complement(30792..30986)
                     /gene="rpsU"
                     /locus_tag="PLM79_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOV85604.1"
                     /translation="MTEAETKESIEKKIRKLRKDFQKNVQPVLRRHNYYLSKGERRRI
                     KKKKAIKRIQRRERRLMRSA"
     gene            complement(31206..33248)
                     /gene="fusA"
                     /locus_tag="PLM79_04535"
     CDS             complement(31206..33248)
                     /gene="fusA"
                     /locus_tag="PLM79_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOV85605.1"
                     /translation="MSDVRRLKTYRNIGIIAHIDAGKTTVSERILFYTGRSHKMGEVH
                     DGTAVLDWMEQEQERGITITSAVTTCHWRDHEIHLIDTPGHVDFTIEVERSLRVLDGA
                     VAVFCGVGGVEPQSETVWHQADRYHVPKIAFVNKMDRVGANFQRVIEQIEQKFRTVAL
                     PLQFPVGREADFRGVVDLVQMKQIVWRDETLGSEYEFVDIDPEILGEVTDARDRLVAR
                     VAEWDDQLMERYLQEEEISAADLIPLLRRLTLQLKIVPVLCGTALRNKGIQPLLDAVV
                     HFLPSPLDVPPVEGMNPVTRQNEHRLSSDQDPLAALAFKIFMDQGRKLTYLRVYSGKL
                     QVGMDVLNASKGSREKISRIFQMHANKRERIEKTGAGDIVAVMGLKSCSTGDSICDPA
                     RPILLEPIGTYEPVISIAVEAKTRGDQEKLLDSLAKLSEEDPTFKHQENPDTGQTIIS
                     GMGELHLEIVLSRLERDYHVGVNAGKPQVVYRETLAEESWAEGVFDREIAGVRHFAGV
                     QVHLRPRERGAGNRVAVRVRDGSIPEMFYPALEEGIWDAVGSGPLLGYPLVDVDAVVF
                     GGTFLEGVSSELAFRVATAMAFKNACEGGKPLLLEPYMSVEVLAPEEFLGEVIGDINA
                     RRGRVESITSRTAIQVVRAVVPLSRMFGYSTALRSATQGRATFTMHFSRYDEASGS"
     gene            34007..35062
                     /locus_tag="PLM79_04540"
     CDS             34007..35062
                     /locus_tag="PLM79_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOV85606.1"
                     /translation="MAPKAKFWRHQLGKSTICRRHPLLYGTLPLFLLLIACFTTQIEA
                     RTEPDTWSELSISFLTPEKISHAGSQMLTSLSPPFAVYNISPVGRSRNRVPIHIPNEP
                     SVRKYIQYYRKEGRATLAGTLKRSSPYVPVMAEILESNGVPAEMIAIVMVESSFKARA
                     AYRGAAGYWQLLPGTARSLGLRVDRWVDERADPIKSTQAAAKYLRSFYDQYQCWTLAI
                     AAYNTGDAPVTRAVKKCNATDFWELYRKSAAPSRSRTYVPKVLAAMHILRNLEAHGFE
                     YPRNFPVYDFESIWVNSPLKLEQVAQWINVPIGQIRDLNSSLVHDRVPPENGFALRLP
                     SGTRDKFDLAYEDYLKR"
     gene            35142..35315
                     /locus_tag="PLM79_04545"
     CDS             35142..35315
                     /locus_tag="PLM79_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85607.1"
                     /translation="MLATELYRAMRTVEEIKKKLEETGLHPSIRGELEDQLRVARAER
                     DRIKALMEGAKAS"
     gene            complement(35995..36516)
                     /gene="cobO"
                     /locus_tag="PLM79_04550"
     CDS             complement(35995..36516)
                     /gene="cobO"
                     /locus_tag="PLM79_04550"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOV85608.1"
                     /translation="MEEGLLVVFTGNGKGKTTAALGMALRAAGHGMRVLFLQFIKGSR
                     SYGELHAVQKLDCIEILPLGTGFTWQKESLEEDRQLARSGWEKAKEAIAGGAYGLIVL
                     DELNYVLSYGLLPWEEVLEVLEHRPPGLHVVVTGRNAPKALISLADLATEMVPLKHPY
                     HDRGLKAQRGIEY"
     gene            complement(36704..36967)
                     /locus_tag="PLM79_04555"
     CDS             complement(36704..36967)
                     /locus_tag="PLM79_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV85609.1"
                     /translation="MPIYEYECLKCGKTLEAMQKFSDPPLTQCSHCKGELHKLISMST
                     FHLRGSGWYVTDYSGKNQSCSASKGEATKKETKASTGEKTAAD"
     gene            complement(37210..39141)
                     /locus_tag="PLM79_04560"
     CDS             complement(37210..39141)
                     /locus_tag="PLM79_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HOV85610.1"
                     /translation="MMRQSPWFSIFTVVLFLSSCAPVSQSPPTSAPVRTLSPAPPGAE
                     ALAGKAEQEYKAGKTAAAISMWERIIQTYPDTALAAKGFHRVGQIYLQMGQPDRALQY
                     LDYLVYAFPGWEGINAARLDRFRAMAAAGHKKQMMKEVTSLWDASSGQPEVRRGLSEL
                     MVKILAEEGDVETGFEWAAAGFAAAKTPAEQEGLVKPTLDLLKDANESKVTRLMKKES
                     SSFMRVFLEYRLVQLDSRKGDAEAGKERLRELLKQNPTHPLASEIRESLRGGPGTGAP
                     AEVEIGVRAERVGCILPLNGQYEKYSRLVLRGLSMAMEDWKAGHPDKPVTLVVKDAPA
                     ESGRSGKAFEDLVEGDQVLAVIGPLGIQSAKEVSPIANRWGVPLLALTQRDEESADHS
                     FVLHVFLDNHDLVQTLVKYCREKLGYTRFAVLYPDDRYGLRLSKVFSEVVQEQGGNLL
                     ASVPYKDRSTDFKEPIQKLMNLAKQNVPPTGVDVTPFEALFIPDQVQTVTLIAPQLPY
                     NNVVGATLLGTNLWGEAPLVQAGGAYVEQALFATPFFAEAASPQVRAFREKYQRFYGA
                     APSYLEAQAYDAMMLFLQARYAGGASNMDRAGLLQNLLQIRGYEGIAGSYFFTPGGNV
                     ERSYLILQVSGGQLVQVAP"
     gene            complement(39278..>39804)
                     /locus_tag="PLM79_04565"
     CDS             complement(39278..>39804)
                     /locus_tag="PLM79_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOV85611.1"
                     /translation="IEVTFDIDANGIVHVSAKDLATQKEQSIQITASSGLSQEEIQRL
                     VKEAETFAEEDRKKRELIEARNQADTLIYSTEKTMREMGDKVDPATRQSIESQIETLK
                     KAMEGTDRDTIQRETDQLMQISHKVAEEAYKRASAQQQETAQSGGGPEGAASSGRKPE
                     DDVVDADFEEVKDK"
BASE COUNT         8068 a        11941 c        11517 g         8278 t
ORIGIN      
        1 agggagcagc cgtagcccag gagactcacg cctttgatcc accgggcact catctgcttg
       61 aggtccccca tgaccatcag ggtccctgca ggagcacggg ggaccccgtt ttcggatcgc
      121 ggaacgtggg gattgtgctg agaaccgggc catacgacga aaccctgggc tcctcccaga
      181 aaaatccggg tccccaggcc caccgttcga tagaacgggt cgttcaaaag tgggctcagc
      241 tggcccgagg tcgagtagtt ggcgttgccc atcctgggct tgagggtccc catgtaggtg
      301 tagatggtct tctgagaaag gtttacggca acgttgtagt tctggtaccc gttacggggg
      361 ttgcagagga ccgcataggg aaggtccttc aacgtgacgg tcatctcgac cttgcgattg
      421 ggatagcagt cggttccgta agcctcggcc ctcaggcgga ccggcttgcc gtccaccagg
      481 tcgtgaagaa catggccgcc gccgtactcg aaatggccgg gggcaatctt gttcaaggga
      541 tcgtcgaaag cgggctccgt ggccccgatg tacagatcca ccgccgccag gcccgcatag
      601 gtcggcacat cgttgagcca aacccgggcg gctttgatgg gaggcttggt gtgcccaaaa
      661 ttgatgaacg cgcccgaaga acacatagga gcgaaggttc cggtggtgac cacgtccact
      721 tcccgggcgg cccgggccgg tcccttcttc ttcacgatgc cgatcatttc ctcggccgtg
      781 accacaaccg ctttcccctt gcgaatcttc tcgttgattt cctggtaggt cttctgtact
      841 gctcccatgg ctgtcctttg tgcgggaatg atactccgcg cagttcgaat caacggttac
      901 cgagcctcct tctcgattct tgggaccctt tggttcccac aggcatcgag ggctctctcc
      961 cgcgccgaca aagccggacc cgccggccgc cctacggctg ggaatgtata acatgagacc
     1021 cattgccgat caatccattc agattccgcc aacgaaccaa ggggatgaga aatcctccgg
     1081 gacgaggaaa tcaggaattc gagggggtcg ccggatcggc ctccaggacc tttaggagcc
     1141 cttccggcac aacccgggac ttccaggttc ggtcgatggc aaccatggtg gcacggccct
     1201 gggcgatgag atcccttccg tcggccgggt agaattggaa cccaaaggtg agggatttgg
     1261 gtttgatctt ttcgacgaac acaaggagcc gaagctcggc cccgtaccac acgggccgat
     1321 agaagtcgca ggacgccccg gcgatgggaa gaccgtatcc ttcttcggga ttgcggaagg
     1381 tcttctcggg ggccaaaccc cgggatcgga ggaattcctc gatgccgcgg tggaaatagc
     1441 ggaagtagga aacaaaatac accactccgt aggggtcggt gtcgccaaaa cgcacccgga
     1501 tggaaatctc cagggatcgg gggacggaca cggtacgagc gtcggtcata gcggcctcct
     1561 cgggtttgga gttgggggta cgaggccggg gggaacgacg gccccgcggt tggttcttgt
     1621 gttcgcacag ggtgcggcct ataattcgat gtatacctca ggaggacgac gtttgggcgc
     1681 aaaaagtcgg ggcggcgctt catctgaatt ttgaacgaga gaaacgagga ttgccttgag
     1741 cgacaacgat cttgaatcta tgcccgcaga accggagagg gcactcagcc tctacgagga
     1801 cggaatccac cgggccctgt ccctggacct ggagggagcg tcccggcgct ttgaagaggt
     1861 gattgccctg gaccctcgtc acgtgggggc ccggtgcaat ttgggagcca ttcttcggct
     1921 ccagggaaga accgacgaag ctcttggaaa ctttcagcag gcccttcgca tcgatgccgg
     1981 gcatgcccgc accctgtcca acctgggcgc cgtattccac gatcagggca ggatccagga
     2041 tgctgagctt gcgttttgcc gggccatccg gttggacccg accctggggg aagcccacta
     2101 taatctctcc cttctctact gccgtcggca acagttcgat cgagcctgga atcacgctcg
     2161 ggaggcggcg gggttgggaa tgagggaggg agcggtgctg ctggaagaga tcgaacggct
     2221 catggagcgg tgagcccgcc cggttcctcc tgcctcccgg cccggaaccc atgtgccgtg
     2281 ccgctttgcg ggccctccac cgcagaaaac gttgccagtg ctcaatttct ttcccttggg
     2341 tccgtgaaac catttgacaa ggcgaaccgt tttttgtagg atcttgccac tcgatatgga
     2401 gtgctggcaa cctccggttg catctcgaat ccctcctctt atgcggcttc tgtgcggacg
     2461 ttgaacggtt gtttttggcc gggcatactc gtcgagaatt cacagctttt tatggagtca
     2521 agggggcggt tttggggccc ccgaagagcg ggtcggaata tttccggcat gtttcagttc
     2581 ctcgtaacac gaattagcag cctgcatcaa tttctttatc ttcatcaaca gtatcggaag
     2641 tctccgccgg ttttggtccg gggagaggga atcattcccg ggcggttcgt cttgctgtga
     2701 gcacaaagag ggaagatcga tcggcagaaa tgagggatgc cgcagaaggg ttgctatctc
     2761 aggcaagaat cttgatccca ccaggccatt tcaacgcttc tttcgggtgc gccggcaagc
     2821 cgggggaagt gggtggggct tggtccgttc ggtggatata acatccgccc tgcagcatcc
     2881 tcaggtggag gttgccggac gacctccacg ggtcctccct ataaagtcac tacattctac
     2941 tgaacgactt tcgaaaatta ttgagagcaa gagtcatgcc catctttcct cgcgaatgcg
     3001 atgaatgcgg caagcctgag gagagcttca ggttttgtga ggaccgctgt atgttgcaga
     3061 gctgtcccga atgcacgttt ttgttgcagc tctacggcac tggatgcgga aatgtccgcg
     3121 tgtattattt gtgtcgtccc tgttatgaga aacgcattgc tcggggctat gtggagccct
     3181 aggaaggagg tgtaggctgg acagtgcaag atctctgtaa acagcggatt ttgcaggctt
     3241 gcgcttggat gcgccggcaa gggggacaac gcctgcatgc ccccaaaatc gagaacatat
     3301 gcaccgtgta ggccccagac atcggaaaac ccaacactgt ccagagtcga aagctactat
     3361 gcataacctg tcggcgttac gttaacttaa cgggaaaaag gaggattaca tggatatcga
     3421 caaaaggatg acgctcaagg atgcggtggc aaaattcatc catgatggcg atacggtcta
     3481 ctatgggggc ttccagattg ccgtccctat ggctctgact cacgaaatca tcaggcagaa
     3541 gaaacgacac cttactacga tagaatcctc cacggacgtc ggcggcctgg accttttggt
     3601 gggcgccggc tgtgtttccg aaatgcatac agcctgggtc atgaactggt acgtgaaagc
     3661 cccctactgc attcgccggg ctttcaacaa aggccacctc aagaggtacg acatctccaa
     3721 cttcggcgcc acctccgcca tgatggcagg ctttctcggc attcccttca tccccatccg
     3781 ggggaacacc ggcacggata tggtcaaaca caatctcatg gatctcagga cgatccaaga
     3841 tcccttcacc ggccagaaca ccaccgtggt gcgtgcgtgg cgcgccgacg tggccattat
     3901 ccacgcccag aaggccgacc gtctgggcaa cgtggtctcc tacggcacac ggggtgtcac
     3961 ggacgagttt ggcgccatgg gagccaaacg gggtgtggtc gtcaccgccg aggagatcgt
     4021 ggagccggaa gtcatccgca gcgacccgga ccgggtcatc gttccctact acaagaccct
     4081 ggccgttgtt cattgccctt ggggcgccca tccatcggcc agccgcggct actacggttt
     4141 ggatattcgg ttctgccagt atcagggcag atacgagcgg cacgcggagc tcctgcctta
     4201 ctttctcgat gaatggatct acggctgcga ggaccacgct gcgtacatga agaaatacgt
     4261 agacaccttc ggccaggaag ccctggaccg tctaaagccc attctcgggt tcaagccgaa
     4321 gctggatgtg gactacgggt accacgatcc ggcagtctgg aagggtgtgc ccatgtacac
     4381 ggatgaccgg ctcaaggcct acgaagaggc actcgcgaag aaagggggaa aataatcatg
     4441 gccgactata aactgaccga gctcatgaca atccgctgtg ctaaggaaat caaggacgga
     4501 gaggttgttt tcgtgggagt gggacttccg atcgttgttg cggccttcgc ctttgccacc
     4561 cacgctccca acatgatcat gttcaccgag ggtggtgcca ttcgggccac ccctccccgg
     4621 ggtctcgcca tcactgtgga tgacccggct cttttcaccg aggctgacgc caccttcggc
     4681 atgctggcca ccatggcagc cctccagcgg ggcgaggtcg acgtggcctt catcggcggc
     4741 gcccagatcg acaagttcgg caacatcaac tccacgggcg ccggcgactg gtccaaaaag
     4801 gagagcttca cctactttgc cggaagcggc ggggccaacg acatggccac ctccggcaaa
     4861 cgagtcctcg ccatcatgcc ccaggacagg aagaagttcg tggagcgatg cgaatacatc
     4921 accacccccg gttacctcga cggaccgggg gcccgcgaaa gactcggcat gatcggagga
     4981 ggaccggact gcgtggtcag caccatgggc gtctaccggt tcgacgaaga caccaaagag
     5041 atgtacttgg cggagtactt ccccggccaa tccgtggaaa agattaaggc caactgtgcc
     5101 tgggacctca aggtgtcgcc ttcggtcatc gagaccactc cgcccacgga agaggaggtc
     5161 aagatcctca gagccattga cccgacaggc ttttacctgg gctagcccgg agacaagcca
     5221 aatcgcatta tgaggggaga gcggtgtttt ctcgacaccg ctctcttcaa agaaacgccg
     5281 cggatacgcg tccggctgag gtcctcacaa agaggccatt tcaacatcca caagaattcc
     5341 cttatcaaaa cagtccggca atgacgattg tgtctatggg gaacgaaccg gggtaggaac
     5401 gaaaagcgca cggcggaatg atccatcccg gcctgggggt aggcacggag gggatgccct
     5461 cctgcagggt agaccaccgc gcggaatcga ccaccgtcga caggggacgt catggaaccc
     5521 aagatcctcg ctgcgggcca caaaagcggc ggccgaaaca agttggaaga aaggtacctg
     5581 aagtaccggc attactacgc aaccatcgac atctgcagct ccggagagta cggaaaagca
     5641 gtgtatgcgc ctcttcttcc tgctttcgat cagtggtaca aaaggaaatc cctcagcgac
     5701 gttcctccca atataccttt cctggaggat cgactctacc ggacggatca ccctgatttc
     5761 agagaggagc ggggaggaac gatcagtttt cacataaagg tcctggccct cagcatgatc
     5821 gggctcatca tgaagtacaa ccgaaaggtc attaccgatc aaattctcaa ctacatcctc
     5881 ttcgaagtct cccagcagac caagtcgtac aattactccg aatgtctatg cttcatgatc
     5941 accgtccaga tgttcatcga gatgctcaag gaagactttt cccacttcgg aagccaggta
     6001 tttgacttga ccctttccga ttttgccaat gaagaaacgt tgaacccaaa gccccagccc
     6061 gtcatcagca ttctgtagga aaagaggaga gttaccgagg aacccatgga agagacgcta
     6121 aagggagata atgcccggga tcccgaccca cgagaatccc tcgccgaacg ccacctcgag
     6181 gaactggcct gcctcgtcga cgagttgggg gactctcaac tggtcatcca ggatcacgat
     6241 cttctcgagt gggctaagaa ccacggcatc tcgccccgag atgccgtggt cgaggtgctc
     6301 cgaagccgaa tcgttcccct gcgctatgcc ggaaacgtgc gtgccctgag ccttgcggaa
     6361 cagagacgcg tgtgcgaaag cagggtcctc gtctgcggtt gcggaggagt cggcggggtc
     6421 ctcatcaacc tgctggcccg ggcgggagtg ggcaccctgc gcttggtgga cggagcagtc
     6481 tttgtcccct ctcacctcaa tcgccaatgg cattgcgaca cccgggagct ctcacgcccc
     6541 aaggctgagg cagccgggga acgggcgcgg gccgcgaacc ccctgatcaa ggtcgaggtg
     6601 tttcccgtcg ccatggacga aaagaatgcc gaatccctgg tggaggggac ggacctggtg
     6661 atggatgccc tggacgacct cccggcgcgt tttgtcctgg cagagacggc caagagcaag
     6721 aaagtgcctc tcattcacac cgccgcggta gggtggtggg ggcaagtgag cacctttctt
     6781 cccgaatcca ttttcgatct gcgcaacgtc tatggtcgag gggaagaaca gagtcagtcc
     6841 gatgagtgga tgggcgccct gggaccggcc cccaccgtga tcgggggact cgccgccttt
     6901 gaagccctcc ggctccttgc cggccgcaat cccgcgtacg cggaccagct ctgctacctg
     6961 gacggcgaaa gcgggcgatt ggatgtcatt tatctcgaac cgtgagccgt cgtcctctat
     7021 ttcaggtatg ggttgtgaag cttttcatgc ccgatggtgg atgagagagt ggacccgtag
     7081 ttgtgtcccg gcatgacgac ggtatcgtcg ggcaggttga agagcttttc caggagggaa
     7141 cggcgcagca tggcccggga gcctcccgga aagtcggtcc tccccgcgga ccccacaaag
     7201 agagtgtccc ccgtgaagac ataccccggc gtatagagac agatgctgcc gagggtgtgc
     7261 cccggggtat gaagaacccg caaggaaatg cttcccacca caatgtcgtc tccgtctttc
     7321 accaggagat ccggataggg agatttctcg gccccgtgca tccggtagag aggaagcggc
     7381 gtaaacatca ccagttccgc gtcctgttcg tggacgacca accgagcccc cgtcttttgc
     7441 ttcatttccg cattgccggt catatgatcc acgtgcccgt gcgtattcac gatgtactgg
     7501 atctccaggt ggttttctcg agcttgccga agaatgtcgt ccactcttgc cgccgggtcg
     7561 atgaccaggc cttctccggt ttccgggtcc ccgacgaggt aggcaaatac ggccatggga
     7621 cctacctgca tctgttttac gaacatgtcc gtccctcttc catctgaagg cagcgcggcc
     7681 ctgaggctcc ccggaccgcc cgataccact cccactgggt ttttttgtat caaaccgccg
     7741 aggaggaatc aaccgggccg ccgcggacca ccatccgggt tttctccatg gccgtagccg
     7801 catttcaccg ggagggtgca cgggttcgtc aacccaaaca tttattcggg ttgacaaaac
     7861 atgcggctcc tgctacgagc atttgtgtgg aggccgagaa tgcaacccgg cttccggcaa
     7921 tcgtctcaat cggaaaatca gccctggagc tcccatggat cccatctccc cgcgaacgcg
     7981 atcactggaa gaagatgaca agaaatacct gtggcaccct ttcacccaaa tgcgcggctg
     8041 gatgcaggaa gaaaccctca tcatcgagga gggagacggc tgctggctca aggacgtgga
     8101 tggcaggcgg tacctggacg gggtctcgtc cctgtggacc aacgtccatg gacatcgaaa
     8161 ggaacccctg gatcgggccc taaaggacca ggtggacaag atcgctcact ccaccctctt
     8221 gggattggcc aacatccccg ccatccaact ggccaaagaa ctcatcgaga tcgctccccc
     8281 gggcctgacc cgggtctttt attcggacag cggttccacg gccgtcgaga tcgccctgaa
     8341 aatggccttc cagtaccatc agcagtgccc ccgaggaaat ccttcgaaga cccggtacgt
     8401 ctgtttgtcc aattcctacc acggcgacac gatcggttcg gtgagtgtgg gtggaatcga
     8461 cctgttccat tccatgtacc ggaagttgct ttttcctgcc taccgggccc cctccccttt
     8521 ctgctaccgg tgttccctgg gcaagtcccc cggttcctgc tccatggact gcctcctgga
     8581 aatggagaag atccttcaac aacatgcccc ggagatcgcc gcggtaatcg tcgagcccct
     8641 ggtccagggg gccgcgggga ttctggtgca tcctcgaggc tacctgcggg aggtacgggg
     8701 actttgcacc cgctacgggg tcttcatgat tgccgatgaa gtcgccgtgg gctttggaaa
     8761 gacgggaacg atgttcgcct gcgaacagga aggggtgacg ccggacctca tggccctggc
     8821 caagggctta tccggaggct acctgcccct ggcggccacc ctggccacag aagagatcta
     8881 cgaaggattc ctgggagaat acgaagaatt caagaccttc tttcacggcc acaccttcac
     8941 gggaaacccg ctgggatgcg cggtggccct ggccggcctc gaagttttcc gccgggagaa
     9001 cgtgctggat tccctccagg ggaaaatctc ccacctggcg cgaaacctcg atgccttccg
     9061 ttccctctcc cacgtgggag acgtgcgcca gtgcggaatc atggtgggga tcgagctcgt
     9121 ggccgacaag gcaaccaagg aaccttaccc gtttgggcaa aggatggggc accgggtgat
     9181 cctcgaggcg aggaaacggg gagtcatcat tcgccccttg ggggacgtcg ttgtgctcat
     9241 gccccctctt gccgtctccc tggaagagat cgacaccctc tgcgccgttg tatacgactc
     9301 ggttcgagcg gtcacggaat aggggggcgg gaaaggattg tgaaggaaga gattcggacc
     9361 ggcagtcttc gttttgaccc caccgacccc atttatgccg agcatttccc ggatcgaccc
     9421 gtggtacccg ggagcctggt ggtgggggct tttctggaaa tcgccgaggg tatggggttc
     9481 ctgggctcgg aagttgccgt cgagaatttt cgatttcggg agtttgtcgt cccgggtctt
     9541 tacgactacc gggtggaggc ccggggggat accttgcggt gtttcctgtc ctccggggac
     9601 aagaagctgg tgacgggatc gttgaagaga tgaccttgga ataccccggg accctggctc
     9661 tgatcctggg cggttcctgt gagctggccc tgcacctggc aagggccatg atcggggacg
     9721 ctcttcgacc gatcctcacc tatcgaaacg aggccggtcg ggatcgcatc gtccgcggac
     9781 tcgagggact ctccggccga tatgaatcga ggcgcttgga cctggccgac aggacaaccc
     9841 tcgaggcact cttggattca tggggagagg accccctgga cttcaccgtg gatttcgccc
     9901 acggcgacta tgaggagctg gtggcggcgg cggacgacga tgtcctggaa cggtatttca
     9961 gggagaacgt gtcttcaagg gcttgtttcc tcagaagggc ggccaggatc atgttgcgcc
    10021 gcaggaaggg aagaatggtt ttcgtgtcct ccggggccgc cgagaatccc aacccgggac
    10081 agggattcta cgcggcgtcc aagctggcgt ccgaagcctt gtatcgcagc gtgggactcg
    10141 aattgggaag acgcggcgtc accacggtga tcctgaggcc ggggtacgtc cgcgcaggca
    10201 gaggcagggc ctacctggaa agggaaggaa agcaggtttc ccggaaaatc cccacgggaa
    10261 aggcattgga aggagaagaa gttgcacgga ccattctctt tctcctttca acccatgctc
    10321 gaaacctgaa tgccaccgtt gtaaccatgg atggcggact ctcgtccgga aagtgaaaaa
    10381 ggaagcgcac atggaactct tcgaatccgt tcaagggata ctcaccgaga ttctggactt
    10441 ggaagatgcg gaactgaccc ccgaaaccta cctcatacgg gacctgggtg ccgaatccat
    10501 cgatcttctc gagatcgccg tggccatgag ctaccgcttc aaaatccggg tggaagacga
    10561 cgaggcgttt ctcaagaact tccgcctcca cctcgtggaa gcccaacagg aggaccttca
    10621 gccggcggat tacctggctg aaaggtaccc ctttctgtcc cgggatcgaa tcgaagagat
    10681 cctctcggac ctcgaggggg gccccacggt caaaatcaaa gacctggtct gttatgcggc
    10741 ccatcgctta gacggcgccc cggcggacga aagcgacccg ctcctgcctc tcctttcggc
    10801 gcaggtcgga gtccgggccg tagcgggacc ccgatagatc gtccatgtcc gggaaacgac
    10861 gcgtcgttat cagttcttcc ggggtgattt cctccctggg agacaccccc ggccggattc
    10921 tcgagagcct caaggaaggc agggtcggtt ttcggccatc gagtgcggac ccgcaggtcg
    10981 tggtctgccc cgtggagggg tttcgtccca agtcttttct gggaccctgc aaagacagtc
    11041 ggtacctcca ccggggagct ctttttgccg cagccgcggc catgattgcc gttcgcgagt
    11101 gcggaattgc cggggaggag cgctcgggag cggggctctt cgtgggggtg ggtccgaacc
    11161 tggatatcgg cggcgaattc ccgtcggttg cggaaggcag gctggaccgg cccgacctcc
    11221 cggccctttg gattttgcgg ttcctgccca atacggccgc ctcggtcatt tcgaagctgg
    11281 ctcaaattca cggcgagaat cttaccgtca ccaccgcctg cgcagcgtct ctccaggcca
    11341 tcggcgaagg atttcgccgc gttcgggagg gacacctgga tcttgccttc gccgggggcg
    11401 gcgactctcg attgagcccc ggcggaattc tggcctacag gaaggcccag gccttgtgca
    11461 tcggggcctt tcccccggac ctggccatgc gccccttcga tgcggatcga gccgggttcg
    11521 tcccgggaga gggcggagcc tttttcctgc tcgaggaact ggagcacgcc aagaggagga
    11581 atgcgccgat cctggcggag gtttgcggct acgggagctc cctggacggt tacagcatga
    11641 cggcgccgga cagggagggg aaaggggcaa gaagagccgt ggagacggcg ctgagagaag
    11701 cccgggtgag gccgtcggaa atcgacgtgg tctttgccca cgggacctcg acgcccctca
    11761 acgacgaagt ggaagcacgg ctcatcgagg aactgtaccc ctcgggctcg ccgcccgtca
    11821 cggccttgaa gtcctggatc ggccacatcg catcggcctg cggcgccctc gagctcggga
    11881 tcgccctctg ctgcatgcgg gaggggtacc ttcccgggat ccgcaacctc cgggcaccct
    11941 gcaacccccg ggtccggttt cttgactccg acgtgaactt gtcctgccga acggcaatcc
    12001 tcgagaactt cggattcggc ggacagaacg cggcccttgt gattcgacgc tacgagtccc
    12061 atgcccgagg agaataaaga ccgtggaaaa ccccctggtg attcggtcca tgcagacggg
    12121 ctttcttctc ttggaccgga tcctcgaggt cggcgccggg aagatcgtcg gggagaagac
    12181 tttccacggc ggccccgcct acctgggaat cgaagccctg gcccagctgg gggcctatca
    12241 cgtccggttt cttaacgatt tcaggaaaca cgctttcctg ctgaagatcc agtcctgcac
    12301 aatcccgacg ggatcaaccc tccggggccc ctatcgtctt ttcggcgaac tgcgagccgg
    12361 cagcgctcat tcgtatgctc atcatctcga ggcccggcgg gaggacggga tcgtctgggg
    12421 aggagagttt ctcttttcca ccgtcgaata cgacgatcgt ttcaagaaag acctccttca
    12481 aactcattac ggaaagatct tttcatgttt gcgaagcgct atgaaaaccg gctgaacctg
    12541 cagaagaatg cagggctcta tcgaaaccct cccgaattcg accgcagaga ggaccgattc
    12601 cttcttctgg gtggaaagaa agtcctgaac ttcgcctcca acgactactt gggactgagc
    12661 accacggaaa tcgtcgggac ccaggtcgct cggaattttc ggaaattcgg ttcctcgtcc
    12721 tcttcctccc gcctggtttc aggcaattac tccgtcatca accgggccga ggaagcctat
    12781 gcctcctatt tcgggtacga agcggcggtg tttttcccca gcggctacca ggccaacttg
    12841 gggatcctat ccgctttctt cgaaaaaggg gacaccatca tcttcgacaa gcacatccat
    12901 gccagcagcg tgaaggggat gactctcagc ggggccgcct tttacggcta caaccacaat
    12961 tccatggatc acctagccaa aagactcgag ggaagcggcc atccgcaggt cgcggtcctc
    13021 accgagtctc ttttcagcat ggacggggat ctcttgaacg tcgcgggcct gcgccgactc
    13081 aaggaaaaat acgggttcct caccgtggtg gacgaggccc attccttcgg tgccttgggc
    13141 ccccggggca agggcattgc ccgggaggtg gccgacgttg ccctgggaac cttcggcaag
    13201 gctttcgggt tgttcggggc gttcgtgctc cttccccggc tgttcaagga ctatctcttc
    13261 aacttctgct ccccgttgat ctacaccacg acccttcccg aagcccacgc cgcctcggcc
    13321 ctggacctcc tcgagatcgt cgagaagagc gaggaccgaa ggtctcacct tgccgacatg
    13381 agtcgactca tgcgacgaag actccgtgag cagggtttta aggtttccgg ggacgcccat
    13441 atcctggcct tggagatcgg agacgaagac aaggcagtcc gggtgtcccg aaagcttctc
    13501 gagaacggga tctatgccct cccggcacga tatccgacgg tgcctttgaa acaggccatt
    13561 ctccggatcg ggattacggc cctgcaccgg gaagacgaca tccggctgtt tgtggacacg
    13621 ctgaggagaa tctgcgatga gctcgaatca agaccctgaa atcctttctg tggtggggac
    13681 ggatacggga gtcggcaaga ccgtcctatc cctgcttctc atgcagttct tctttgccaa
    13741 agggctcaaa cccttctacc tgaaacccgt ccagacgggc tgccgggacc cttacgatac
    13801 cgacagcgac gcacggttcg tctacgaaca cgtggaggct ctccggggtc gggacccggc
    13861 cgaatccgtg atctattgct tccgggaacc caaggcccct tactttgccg cccgcaacga
    13921 aggaagcacc gtcgatcccc gggtgattcg aaaggtggtg gaggaaaaac ggcttcatca
    13981 ctcccccctg gtcctcgagg cggcgggtgg gatcctggtt cccatgacct ccacccaaca
    14041 tgggatcgac ctggcggccg aaacgggcgc ccggcccgtc ctcgccgccc gatccgccct
    14101 gggaaccatc aatcacacgc tccttaccct cgaggccctt cgagccagga acctcgatcc
    14161 cttgggcatt gtgttcatcg accccttcga cccctccact cccagggacc tggtcgagga
    14221 aaacatggaa gccgtccggg attttggcgg gatctccgtg gcgggagtca ttccccggat
    14281 tccggatttc tcgaaccccc cgggggagtg cttccaagtc ctgggccggc tgcttgccta
    14341 gaaacggctc tttccttact cgccccgacc cgtgaatcct attccgatgg ctcttgtctc
    14401 ccccgcgcgc atctgctaaa cccatacttc ccggcaacga agatttccgc ccccggcagg
    14461 aagcagccca tacaccaagt tgcgcccaag gtcgctttga tatgctgagt gaagattctg
    14521 ggtagatggc tgaatcctgt gggcgggaag tttgggtgtg tatgcaactt cgtagtaaag
    14581 ccttgaaatc gtccaacgaa aggaatccat catgcccctc aaaccggcct ccgatgagcc
    14641 cccccgccag agtccgtccg ccccggtgcg caacccgtat atcctgaact tctgcaaggt
    14701 cctggtagaa aaaaagggcg aacagcaccc cccggatgct ctgaaaaagc ttcttcacga
    14761 catgtatcgt ctcttcgagg ccatgctggg acagaacatg gtccgggcac ttcccgaaga
    14821 cttgcgccgg aagtacctga ccctgtgcga agacctcagc cggctcaatt acgagaccat
    14881 cggggacatc ttcgacaaga acgttcccca gtacgaacag gtcatgaaat ccaccatgaa
    14941 acagttcgcg gaaatcttca tgaggaaccg ggtattcaat cccaaagact acccggtccc
    15001 tctcaaagat gattcggaat agcggtaaaa ggagtcttgg ccatgccgga acccgaagac
    15061 aagaccattt gcccccactg cggggttaag atgctcaagt ggaagacccc catgctttcc
    15121 acctggagcg ccgagttttt ctatgtgtgc ttcaacgacg attgccctta ttttgtccgg
    15181 ggctggaacc acatggaaaa gaccatgcaa aacacctgtt cctaccgcca tcgatacgac
    15241 ccgaccacgg gagcctgcgg gcctcttccc gtctggtccg tcgacgccac ccgctcacgg
    15301 atcgtggacg actccgaaga gggttaggtc cgccttttgc ccacatctgc gatcaggcgg
    15361 ggatagatcg ctgcctccga gcgaacccgg agggttcaca accattcttc gggggtcgag
    15421 gccgcaaggt cgatattcct ggaacaggtc ccaagaggaa gtcgatccat ggggtcgcag
    15481 gaccgtcccc tgggcaagag tgggattgcg acaagtccgg taggggcggc cggttgcccc
    15541 tacgccctct gcacgcacgg aaaaagccat gcggggagat ttgccccccg gatccagcag
    15601 cgccgctcgg cgaaaggcat cgaattgtgt tgagggcgag cttcctgata agatctcacg
    15661 gactgcctgt gcgcctcacg ttgaggacgg gctttttcag gaaggtaaaa gacagccatc
    15721 ggaacgcgag ccgcagcagg ctttcatccc ccacccggat ggcctcgatt tcttcttcgg
    15781 gcaggtcaaa gagctgcagg aagcggcctc ccagtacgaa ggaccgaaac cggtccaggt
    15841 cgtaggcagc gaggaagaac atctgctgct gtcccagggt gaagccttgg gcacggcgga
    15901 tggccgggtg ggtgacgatt tccatccaag gattgttcat ttcatggtag gaatccagtc
    15961 cctggtctga gatccaatcc ctcacgttcc actcacgggg ctcggaaaag ccccggcaat
    16021 gctcttctct cagcaagtag tagtgttcct gcacggcgtc gtgcgtgggg tgcacccggg
    16081 acgcccgagc gacgggataa atcctgcacg ccgagggcct cccctcatag atcctgcagc
    16141 ccgggggaga aacgaacggg caggtcttgc gctcgttgtt tgccatccga agataagcca
    16201 tgggcaaggt tccctgctct tcggggcgga tctccgcgta ctcttccagg aaatccccgg
    16261 aagtcatccc cagctggttt ttcatccgga tgatgtcgta gggagtgagc tgcaatcgca
    16321 ggtcccggca gcactcggta aaacagggca ccttcggatg gcacgcaaag cggaatcgcc
    16381 cctccgagag gggctccagt cggtcttgaa tggtcatcgg ggcggtcttt tccagcgaca
    16441 tgttcatcct tgctcttggg tccgggggtc tgggggaccg atctcacagc agcgcagaca
    16501 gctcttccat ctggcttcgg atgtagcgaa aggccaggcg cagcagggcc tcgtcatccc
    16561 ggagaaccgc ctcgagcgtg gatgggtcca attcgtagaa gcgctgcagc ttctcgtccc
    16621 cgaggagccc ggcgaaccgg tccaggtcat aggccagaaa caagagcctt cccagccccg
    16681 gttccatggt tcgcccgggt tctgtcccgg gaggaatcac ccgttggaag gcttcctcca
    16741 tttccaggta ttccttcact ccctggcctt cgagccactc ccccgcggac cgaacggctc
    16801 gttctcgaag ccccaggcaa cggtccttcc ccacgataag ccgatactcc ccctgccggg
    16861 gcgtgagatc caggggatac atgcggcaag cccacggcct gtcttggtac acccggcatc
    16921 cctggtcggt cacgagagtg cagctgaggg tccgtggatc catcaggagc tgaaccacgg
    16981 gaatgtgaga cggcccggcc aggtaatggt gggtgtgcct ttcgagcagt tcccgggcgc
    17041 tgagtcccag ggacctccga agccgcaaca catcgtaggg cgtcagaaag atgttcacgt
    17101 ccctgcagca ctgggtgaaa cagggaagat ccttgcggca ctggaaagga aacgggcttt
    17161 gggattggag agtgccggtc gatttctcat cgagcgaata gttcatgaaa accctcggaa
    17221 tctattgctt caagaggtcg gcttcaaagt ttcgaaaaga aggattcccc tggatgaatc
    17281 gccgaaccac ggaaagagtc gtggaatcct cgcagacaaa gtaatggatt gtacactgcc
    17341 gttggcattt agggcagaaa tagtcctcgt ccagggcttg tccgcaggaa caaaagggct
    17401 ccatggtcaa ttgatcctct tcccatttgg ccaggaaaaa gtcccggccc accacattct
    17461 ccgcgtcctg ctttgatgtc atcgaaagca tcttcctttc ttggctgtct cggcccccag
    17521 ggccgtcccg tcgaagtgtt cccttttaga tcacaaagcc ttggcgagga cttccgagag
    17581 ctcgaggatt tccagggatt cctgttttcc aaggctggtg caggcatcgg tgagcatgtt
    17641 gacgcagtag ggacaggcgg tggtcaggac ttgggccccg agctccaccg catcggtgat
    17701 tcgaagctct ccgaaccgtt cccccatggg aacctctccc caaatacgcc ctcccccccc
    17761 cgcacagcaa agactcaggg gaccggacct tcccagctcg agggattcca ccccggcgac
    17821 ggatccaaga agatctcgag gatcctcaaa gatgccgctc tgccgtccca ggtaacacgg
    17881 atcgtggaag gcgatcttgc gggcgaactc cccgggaaag gacagcctgc cctccttcac
    17941 cagccgactc agcagttggg tgtagtggga gacttcccac tccccgcccc attgggggta
    18001 ttcgttcttg aaggtccaga ggcagtgggg ggatgtggtc acgatctttc ggaccccccg
    18061 ttcgttgaaa agacgaatgt tggaatgggc caacttttgg aaaagctcct cgtctcccat
    18121 cttgtggatg gtctccccgc agcagctctc ctcgattccg atcaccccga agctcacccc
    18181 ggcctgttga agaagccgga caatgctccg ggcgatcctg gtgctgcggg ggtcatagca
    18241 ggagtggcag cagacaaaaa gaaaatactc catgtccgga gtgaagttgg ggacctccat
    18301 ctttccctgc cactccgtgc gcttctgccg aggtcccgcc caaggattgc cgttggcatg
    18361 ggtgctgccc aggatgggac ggagatgagc gggtgccatg cccgctccca caatgccggc
    18421 ccgcatggcc cggacattgt ccacgatctt cactccccgg gggcagttgg attggcacat
    18481 gccgcacgtg gaacaggcga aaaggatctc ttcctcttcg aaaccctcca ttcccagctg
    18541 tcccaagcgc tgcatgtggc ggatgttgaa cttggcgctc gtttcccccg gaaccaggcg
    18601 ccacgggcac aggttggtgc acaggccgca ttgcatgcac cagttcacgg tttccgcgcc
    18661 catctcagca atatagtctc tcatctccag cgaaataatc ctgggttcca ccttcgatcg
    18721 ctccttttca tcggcccggg gcctgcggtt gaaatgcagg ggattatagc ccgtacacgg
    18781 cacaaagtca ttccggccga cggtccccga tgccctagaa tcccttgtaa ggattggccc
    18841 ccagttcctg gaccctggcc gcgaattcat ccaggattcc gggcagtcga ggatagtctg
    18901 caacggagat ctgttcgagg cggacacgat ccgattccag cacgagtcga ttgagggtct
    18961 cctggatctt gctcatacgg atctcggcca gctcgctccc cttgataaag tgacactggt
    19021 agttctctcc gtgtttgcac ccaaggagca ggatcccgtc gattcccttg gacagggcgt
    19081 cggcgatcca gaccaggttt atggaaccca ggcaccgaac cggaatgaag cgaacccagg
    19141 ggttgtagct cagtcgtttg attccggcca tgtccaatgc cggatacgca tcgttttcgc
    19201 acaccagcac caggattcgg ggcttttcct cgtcctcctc gggcacttcc atgctcttga
    19261 tcatgttgcc caccatggcg accgagtagt tcttgaagga gacgatgcgt tccggacagg
    19321 cccccatgca gaccccgcat cgcctgcagc gggtgggatt tggaagcgga ttggcctttt
    19381 catcctcatt gatggctcca aaaggacact cctccgtgca ccgcttgcac tgggtgcagc
    19441 gctgcatgaa gaattccggg taggtgaggt ccccggcccg gggatgaacg gcttttccct
    19501 cggccaccat ttccacgcac tgaatggcct tgagggcggc gccggttcca tccacttccg
    19561 cccgagcgta atccatggga gcccgaacgg taccggcggc ataaatgccc gtgcggcggg
    19621 attcgtaggg aaagcaaata aagtgggaat cgggaaaacc gtatttcagg gccggaagct
    19681 cgggcccctg ccgatattcc aggttcagca ggttcgaaat gagaacggcg tcttccggca
    19741 ccgacgactc ttcctcccct tccttggggg tttgagcttt gaacggttcc ccgaaagcgg
    19801 ttgtgggaac cattcccgtg gccaaaacca ccaggtcgat gctttcggac aggaccggag
    19861 aacccgtgag cacgtcatcg gattggacca ccagtccgcc ggaaccgtcg tcggagacgc
    19921 tggtgaccga tccgcggaca aaaagcaccc cgtccttctg cagttctttg tagaaaagct
    19981 cgtaggttcc gtgggcccgc aggtcccgat agaaaacgta gacggggacc gcaggatccg
    20041 cagccttgaa gtacttggcc tgcttgaggg attccacgca acaggcagcc gagcaatagg
    20101 gcaaatgccg ggggtccctg gatccggcac acagcacgaa agccacgctt tttaccggtt
    20161 gcccgttgga agggcgcacg ggcccgccgt tttgaaacat ggactcgagt tcggatgccg
    20221 tgatcacatc gggataggtt ccatacccca ggccttccag ccgttgagga tcgtacggga
    20281 cggatcccgt ggccaggacc acggctccca cgcgttcccg gatctccgac ccgttctgca
    20341 ggatctgcac gtcgaacatg catggggctc cgctgatccg gcccacccgg gcaccggtat
    20401 agaccgtcac ccgggaatcc tcttccagtt cccgcaccag ttcttccagg ttgaattcct
    20461 ccggctcttc atagggcgga cccagaggag gaagcttatg aactcccttc aagtagccgc
    20521 ccaggcgatc ggtcttttcc accaggaccg tttcgtagcc ggcctttgcg gcttccctcg
    20581 ccgcagtgat tcccgcaagt cctcctccga ccaccagaac cttcctgctg ggcccctcga
    20641 tgcaaaaagg agcgggcagc tccgtctccc tggccctgat gattcccatc cggagttgat
    20701 cttctgccat cataagagtg tcttcctccc cgggccgatg gcaccaggcc acctgctcac
    20761 ggaggttcac ccgctccacg atgcagtcgg ggccgaagtc aaacacatcg gtcatgactc
    20821 gctgggaaca ggcggcaagg acgaaggtgt tgatcccgga ctccgaacgg tgggcatgaa
    20881 ggagggccag cccctccttg ccgcacaggc actcgtgggt ttgacagacg gggaccttga
    20941 attccccgac ggcgacctta gtcagggcct gaacatccat ggcactccca atgccgcacc
    21001 ccgcacagat ataaacacct ggtttcttct ccatggaatt atctcctgcc tagtgcctga
    21061 atggccttga gagccgccgc cgtggcgtcc tggacgcacg atgccacatc cacggggtgc
    21121 tggacgcaac cggccccgat gatcccgggc gtgccctgcc gggagaggac aaagccatgg
    21181 tcgtcgtagg tcaaggtcgc ggggattttt tccgtggccg tggaaggaac cattccggtc
    21241 gcgagtacca ccaagtcgaa ggcttccttc aggatttccc ccgtcgactg gtcctcgcac
    21301 cggaccacta cccgggagcc gggggggtct tcggtgagtt ccccggcctt ccccttgatg
    21361 agtcgcaccc gctcgtgggc ctgaaccgct gcgaagaagt cttcgtattt tcccggggct
    21421 cggatgtcga tatagaagat gacgacttcc gcctggggat tctgctggag gaggtaggtg
    21481 gcctgtttga gggaggccag gcagcagacc ccggaacaat atggcaggtg gttctcgtct
    21541 cgggacccgg cgcattggat gaaggcgatc ttacggactt cgcccccgtc ggaggggcgg
    21601 acgatcctcc cgccggtagg gcctcccggc gaagccagcc gttccatgat tacgttggtg
    21661 acgacattct tgtgttttcc gtagccgtag taggggatct tcccggcgtc gtagggattc
    21721 caccccgtgg cccacaggac ggcccccacc ttgaggggaa cctcccggac ctgcatatcg
    21781 aggtccaccg ctccgtaggg acaggcgtcc cggatctctt cggcttcccg ggtgccgacc
    21841 acttccgggg acaatacata ccgcatggga taggcgaatt cgtggggcag gtggatggcc
    21901 ttgacccggt cgagcccgta gttgaagggg ttggggaacg tgcttcgagc ggccgcctcg
    21961 caggctccgc acgccgtgca gttctcgttg acgaaccggg gccgaacccg gacggtaacc
    22021 tcaaaattgc ccgcctcccc ctcgatcttc tcgacctccg ccatggtgag aaagcgaatg
    22081 cgcgggttgt tcttgatgcg gcggaaattg atctcgagcc cgcagccggg ggggcacagc
    22141 ttgggaaagt accggttcat ccgggctacg cggcccccca gataggcctc ccgctctacc
    22201 aggatgacct gaaatcccgc ctcagccgct tccactgcgg cgctcaaccc cgtcattccc
    22261 gctccggcga tgaggatact gcgactaccg tttgctgctt cagccatggg acttcctccg
    22321 tggtcggcct tgagatcctt ccgtgacggc aattgcgttt ccggtttcag aacagaaccg
    22381 cgccccgtgg gctcttcgcc cggcaccact gtcccggaac ggcgaagagg cggcagaaaa
    22441 agcggtgaaa ggtggggaat gcaggcgaat ctcccacaag accgtacaga acggtttcgg
    22501 ttcagaggaa acggtcttgc aggataccct atccacgaga ggcttttcaa cggcaaagac
    22561 gcgggaatat tcccgcacga ccggggggaa agccgaggat ttcgcaaccc tctccacgat
    22621 cccctatcgt ctgcatgcac cgggaacggc cgaggatgga ggggacggga ttcccccgcc
    22681 aaatgccgag gatcggaccc cgagcctggg caaaaaaggg gagacggctt tgttgccgtc
    22741 tccccgatgg aggtttcctt cccaaacgtt cccgaggact agtagtcttt caggaagtca
    22801 tccagaacgt cgatgctctt cttctcgatc accagatcga agatcttatc cttcaccttg
    22861 cggggaagaa gctcgatgct tttggtggtg gccgagtcaa acttggccgt ggggaaaacc
    22921 ttgaggatct tatccttttg ggcgtcgttg gcgtaaacgt agatcacctt caggccggcc
    22981 atggcatctt tggtcatgct cgccaatccg gcagcctgga gcttcgcgaa acggtcttcg
    23041 ttgacccaaa gattgattgg atattttccg tcagccatca gaacactcct cttatgcttt
    23101 taagggttcg attgagaaga ccggggtcct cttggcaagg tactcgtccg tcacgaaggg
    23161 ggaaccgaag ccgtactttt ccgcacattc catcaccaag tcgaacactt ccgggcgaaa
    23221 gatgaggcgg ggaggtttga cgccgtcatc gacaatgctg cgaatgaggg taccgctgaa
    23281 cttttgccac tcggacttgt ggttgcaaag accttccgaa gtcacctcac cgcagtgggg
    23341 gcagtagagc cagttcatgg aatacacggg gacgatgtcc agctcatcct ggtacttggc
    23401 caggagattg tgggcatcat aagcaccgta gtaggtcccc acccccgcgt gatctcgccc
    23461 gaacatgtgg tgggtcaatc ccaggttggt gcgcaggatg gcatggaaaa tggcttcccg
    23521 gggtcccgca tagcgcatat cccagaaagt gagggaagtc agatggttgt gatctccaaa
    23581 gtaaccggac ttgcgcagtt cgtcctgggt caggatgatg gcttcgtcga tgtagtcgcc
    23641 tttcctcttc tcaccgatga tggcgtttac caggacgccg accaggggtt tgtcgatggg
    23701 caactcgccg tacgtctggg accaggcgcc cttcatcagc cactcgtggc ccgtgtgggg
    23761 aacgttccgg gtctggtggg ccaccacccg cttccagcct cttttcttga actcctctct
    23821 catctggatg ggcgggatga aataaggtcc gtagggctca ttgatcttgg gcttgttcac
    23881 gagggtgatc ttgcccccga tgaacttttc ctcatatttg tatgtcctgg cgcagcccgg
    23941 gtgtttctct tcggtcgtac cgtagacttt ctccgccatg accttcttgt cataggtgaa
    24001 gatttcgttg atctcgaaga gggccatggg attaccgtcg taggtcagga ccacggactg
    24061 tccctctttg atgccgtact tggcaatatc cgcatccgac atatcgaaaa cgatggggat
    24121 gctccagagg atgccgtttg ccagatgcat gttttggacg actccgtcca cgtcggcttt
    24181 ccccatgaaa ccttccagtg gggagaaaaa gccgtaggag atgccgatga tttcattggc
    24241 gatctggctg cgcacgggaa cctgggtcag tccctgaatc tttttcacgg cctcgtcggg
    24301 ggccataatt ttctcaacaa tttccttctt gccatgtccg ataacggcca ttttgatctc
    24361 tccttttctg tgattttcgc gcacttcgaa ctggccgatt ttctaaaaaa tcgcgctcct
    24421 ggaagcaatc caggaatggg ccgtcgcatt ccggcccaac atagaaaaac ctccgggcac
    24481 agggagcgca cccggaggtt aggactacac gaacttgggt tagaccggca ggttgaccac
    24541 gggaaccttc ttcatggtcc actcacccgt tttggggttc cacaccgaat tgacgaacac
    24601 cctccaattg gcctcatcca tgtcggggag gtccgcacgg aagtagtatc cgggccaacg
    24661 gctttcctgg cggaaaagca catggcgcag gtggctctcg gcgatccaca tgcggtgggt
    24721 gttttcccaa gctctcatga gctcgtggag atcggaggcc gccagctttt cggcgtcttc
    24781 cttgagcatc tgcaacagtt cgaggcctct ttccagaagg gctttgttgg tggtgaactg
    24841 tgcgctcacg cccccgcagt attcatccat gatcttctgg aggcggaaca tgaacatctt
    24901 gggctttatg aagttggggt tgatgtccgg atcactggac agcttggcaa actcctcgaa
    24961 acgatcaagg ggagccagaa tggtcttctt catgctctcg atgacggcat cgctcacctg
    25021 cggggccgtg ttgttgtcca gaatgtaggc aatggcaccc ttggctgcga ttcgcccttc
    25081 ggcgtgggag ccggaggaga acttgtggct ggatgcccct gcgccgtcac cggcggtgaa
    25141 gaggcccttc acggtggtca tgtggttgta cacaccgatg ggttcccact gggctcggta
    25201 ctcagccggc atcacgtcct cgggaccaca tacccaggca ccggaagcgc cggagtgcga
    25261 accgatgaag tagggctcgg cggccgcgat ttcggaggat ttctcttccg gctggatgtt
    25321 catgccggcc cacaggaggg actggctgat ggtcatatcc aggaagtcct cccaggcttc
    25381 ggcttccagt tccttcatct tctttttgaa ggccttggga tcgtctttgt acttggcggc
    25441 caggttggca atggcctctt ccgttctcat gaagatggga cccttgccgg ccatgacgtc
    25501 ttccatgccc agccagttgc gcaggttggc gggcatgggc ttgccttcgc cgtagggagc
    25561 ccacttgggc agctcatcct tccgggtgac catgtattcc tcacccaagg aattggtggc
    25621 ccgggacttg aacagcagga accatgcacc cacggggccg tatccgtctt tgaaccgcac
    25681 ggggatgaag cgcacttcct ggcaggtcat ttcggcaccg gcccggatgg tgaaataggc
    25741 ggtggaaccg gcattccacg ggggatacca tgctcgaccc agaccttcgc ccacggaccg
    25801 gggacggaac acgtgaacag ccccacccat catggcgatg gtagccttgg ctttgaagac
    25861 ataaaatttg ttctcgcgaa cgctgaagcc gacggcaccc aggcacttgt tgttgtcgaa
    25921 gtccatgagg ggctcgacga tgaacacgcg ctcatagagc caatcggtga aaccggcctg
    25981 gttcatggcg ttcttggcgg cctcggccac gatgaccttg taagactcgc cgttgatcat
    26041 gatctgccag cgaccttcat tgacgtagtt gccctgctcg tccttccaga tggggaggcc
    26101 ccatttttcg aacaggtgca cggtggaatc aacatggcgg gcaatatcat acaccaaatc
    26161 ttcacgggag atgcccatga ggtcttggcg gacatatctt acgtagtctt ccagggtgtt
    26221 cacgcctttg gcgtagttga tgtactggtt gatggccgaa agccccatag ccacggcgcc
    26281 ggagcgatcc atggccgcct tgtccaccac gaccaccttg aggttgttct tcttggccca
    26341 gtaggcggct tcaaaggtgg caccacaggc actcattcca ccgccgatga tcaggagatc
    26401 agtgtcgacg actaccgttt caaaattagc catatcttta cctccttggg ttatttcttc
    26461 agggtgggaa gcgtgctctg gccggtggaa gcaggctcgg tgcacaggat tgggctcttg
    26521 atgtcatcgg ttcccgtctc gtagccgccc agaggattgg cttgaccctc gggagtggtc
    26581 cggatgggga acttgaagcg ttttacctgg ccgttacgga acttaacggt ccacatgatg
    26641 tcctcagagc cgcgcagggg caccactgaa gctcccatgg gtacgaaatc ggcatatgca
    26701 cgcacatcaa tggcctgctg ggggcagatc ttcacacagt tgtagcattc ccagcacatt
    26761 tcaggcgccc ggttccaggc tttcatcttt tccttgtcca agaccatcag gtcgttgggg
    26821 cagatgtaca tgcatgcagt cttatcctgc cctttgcacc cgtcgcattt ctccaggatg
    26881 acataactcg gcatagtttt ttcctcctaa aggtttccac ttatcacttt agcctatgat
    26941 gctctgacct tggagtcgac acctaagtcg ggctcacacc tcctttctgg ttccaagccg
    27001 gcctctcagt ggctcaacac ggtaagcagt ctcggaaacc ggcctcaatt ggttccgaaa
    27061 tagccgggtt ctctcgccct aaagcactga aagatgatgg ggctgaatca caatcacagt
    27121 ttcagaggat ctctcgggga acgttgaggg gctgtcgagc ttacaggttt gggcaatatt
    27181 gagcgttctg cccgttcgga ggttcactcc atcatcgtac atatagcccc gatatcccaa
    27241 tcccaagcct agatgctttt ctttgttcaa aacctgtgat ttgcttgtgc aataaattgc
    27301 aaggtgaaat tatagagatc acgcctttct tgtcaaggga aaaatgatgt tcttttcaca
    27361 atctctgatg caaagtctgt tcggatcccc atcgaggccc cgagtaaggc tccgggcaag
    27421 cttccgcggc ccccgaaccc ctttttcttt cgtttttggg cccccaatcc cccgagtttt
    27481 ccttcttcga ggaacaaaaa ccggcccgtg acctttacca cggccccgaa cgatccaaaa
    27541 acggcctttt ccacccacct cttcccccag caaggacccg ggcactttgt gcacaatatc
    27601 cataatgccc aggcccagga agagatattg tgccagcgag cacgaagcag cactccccct
    27661 ccgcttttgt ctccaaaacc ctccctttgc ccaccccgag gtccccacgc cccggctttc
    27721 ccctcccccc gagtccccgg gagaaacccc gtaaaatccc attgaattta cagccgtctt
    27781 tccctagagt gaagagcacc tcaaaaatga acgccggaca atgtagggaa atcattatgg
    27841 aaagtccttg gctgagcact acgggccgac acatcctggc tctcatcctc ctatcgggcc
    27901 tgcttatcgg cttcggcacc gccggataca tcctcatcga ggaattctcc cttctggacg
    27961 gcctttacat gaccatcatc accctcacta ccgtgggttt cggagaggtg aaacccctcc
    28021 agcccatggg cagagtcttc accatcctgc tcatcctcat gggagcgggc ctggtggctt
    28081 acaacctggc ttatttcagc cagctggtcc tggatgggaa tctgctggta ctttaccgga
    28141 ggcgtcgatt gcgaaaacaa ctggaacacg tagcggacca ctacatcatc tgcggatacg
    28201 gacaaatggg caagatcatc gcccaggagc tcagcaagaa ccatgtgccg gtggtggttg
    28261 tggaaaatga cccgtcggcc gtggtgagca tccgggaaaa ggaaatccat tattttgcgg
    28321 gagacgccac cgaggaggag acccttcttt ccgcaggaat tcttcgagcc cgcggattga
    28381 tttccgtggt gagcagggat acggacagcg ttttcatcat catcaccgcc aaggatctca
    28441 acaaggacct caccattttc gcccgggcca caaccccggg ggccgaaaga aagctgctga
    28501 aggcgggagc caccaaggtg gtttcgccct atgccagcgg ggcgatacgc atcgcccaca
    28561 acatcctgag gcccacggtg acggattttc tggaattggc gctgtcggcc gaaggcatgg
    28621 agctcagcat ggaagagatt cgccttcctt ccgaggcgga cctcgtgggc atggagctca
    28681 tgtactccgg aatccggagc cggtacaacc tcatcgtagt ctgcatcaag aggagcagcg
    28741 gggccatgat ctacaacccc tccccccagg aggtattgaa ggaggacgac atcttggtgg
    28801 tcataggacc acaggaaaac ctgaatcgtt tttccgccga tcttttcgga cgtccccgtc
    28861 cttcccaacg gcccggcaga tcttgatccg ggccgcggga cgtggccctc atagtggccg
    28921 acgaggcctt tggtacccag tctttggcaa tggaccggga gatcgggggg accttttcga
    28981 ggcgaacgtc aacgggggcg ccgtcgccct ggggcacctc gttgaagcct tgggagcccg
    29041 gattccggcc cctctgctcc gcgagacggc caaacggggc gccgggaaag ggttggcaac
    29101 accgtatatt ggaggaggcc aggggtcggc gatccgtgga gaacggccct gattcccgcc
    29161 taatcctaaa atttcttttc aaggggaggg ttgtgccatg gagtatgcaa acgtcttgtt
    29221 tgagatccgg gacggcgtgg ggatcctcac cttcaaccga cccaaggccc tcaacgccct
    29281 aaatgttgaa accctggagg aactgaacga cgtccttgat cgagtccggg aagacgatgc
    29341 cgttcgggtg ctcatcctga cgggatccgg cgaaaaggct ttcgttgcgg gtgctgacat
    29401 atccgagttt cccaggatga acgccctcca agcgcaccgg tttgcggagc ggggacaaga
    29461 gattttttcc aagctcgagg agcttcccaa accggtgatc gcctgcgtga acggctttgc
    29521 cctgggaggc ggctgcgagc tggccatgag ctgcgacttc atttacgcct cttccctggc
    29581 caggttcgga caacccgaga tcaacttggg gctcattccc gggttcggcg ggacccagag
    29641 actcccccgg ctgatcggtc gggccaaggc caaggaacta tgcatgaccg gggaaatgat
    29701 cgatgcccag caggcaatgg aactgggatt ggcggccagg gtcttcccgc ccgaacagct
    29761 catggaggaa accctgaaag tggcccggct tttggcctcc cgcagcccgg tggccctccg
    29821 ggccctcaag catgccattg accgcggatg ggacctggat ctgcgaaacg gctgctccct
    29881 cgaggcggaa agcttcggtc tctgcttttc cagccaggat atgagggaag gagtggccgc
    29941 ttttctggaa aagcgcaaac cgaattttca gggaagcctt acccgctgag gcaaaggggg
    30001 acgctccgac gagctggttt cccgcgcagc acaaacccgg ggcccaccgt tctcgatcaa
    30061 aggcaggggg caacctgtgg acggaggacc taagaatctc gatcttaccc agtagaagtt
    30121 gatccaagag acggcctgca ggtttgccga gcgcgagatc gggcctgccc cggccgaaac
    30181 ccggactcac cgtcatcccg gagaaactgc ggcccggatg ggagaagtgg ggtttatggg
    30241 gatcaccatc tccgaggaat acggcggagc cggaatggat tgcatcggct acgtcccggc
    30301 caggattgaa atctcggagg ccgagccccc acgggcgtca tgatgagtgt gccagcctct
    30361 gcggctttcc cgtctgcact tgcggcaaca agggaccgag gaggcggttt ttaaagcccg
    30421 cggcctcgct gccgactctt cgcgccgcct ggctggaacg ggggacggga ctacgagcgg
    30481 gaggccgcca tggacgctgc cgccgaagaa gttccggtcc ttagcggtta cgagtcttgg
    30541 aaggactatc ccagggagcg gtacctgggg gacgaaaaaa gcgctcggac ctgggagggg
    30601 accaacgaga tcatgcgctt ggcgattgcc cgggacctgt taaggaatcg atgggaccgg
    30661 acaggcttgg ggatgggccg gaaagacaaa accctggggg aacggtgttt cccaccaggg
    30721 ttctttttcg gaaggcctgg gtaatctctg gaccaaactt gcagccgtct tggccctctg
    30781 ggacgaacga ctcacgccga acgcatcaat cggcgctccc gccgctgaat cctcttgatg
    30841 gctttcttct tcttgattcg gcgccgttcc cccttggaca agtagtagtt gtgcctgcgc
    30901 aagacgggtt ggacattttt ttgaaagtct ttacggagct tgcgtatctt tttttctatg
    30961 ctttctttgg tttcggcctc cgtcatccag tactcaccta cctttcagaa aagcccctgt
    31021 tggggcggac ataccggagc cgtcccacca cagagcctct ccccggacct ggagtccgcg
    31081 ctccgaattt cgatgcggtg tggacctgtg tcggcagcct gcccgtctct cccataatcc
    31141 ttaaatgcta cttttttccg ggaatcattg caagaaaata gcgctatttt cggaaaaaaa
    31201 cgatcctaag accccgaagc ttcgtcgtag cgggaaaagt gcatggtgaa ggtcgctctt
    31261 ccctgggtgg cggacctcag ggccgtggag tagccgaaca tcctggacag gggcaccacg
    31321 gcccgcacca cctggatcgc cgttcgggag gtgatgctct ccaccctccc cctccgggcg
    31381 ttgatgtctc cgatgacctc ccccaggaac tcttcgggtg ccaggacctc cacgctcatg
    31441 tacggctcga gcagcagggg ctttccccct tcgcaggcgt tcttgaacgc catcgccgtc
    31501 gccacccgaa aggccagttc gctggaaacc ccttccagaa aggtgccgcc gaataccacc
    31561 gcgtccacgt ccaccagggg gtaccccaga aggggtccgc ttcccacggc atcccaaatc
    31621 ccttcctcca gggccggata gaacatctcc gggatcgacc cgtctcggac ccgcaccgcc
    31681 acccggtttc ccgccccccg ttcccggggg cgcaaatgga cttggacccc cgcaaagtgg
    31741 cggaccccgg caatttctcg atcaaacact ccctcggccc aggattcctc ggcgagggtc
    31801 tcccggtaaa ccacctgcgg ctttcctgcg ttgaccccca catgatagtc ccgttccaac
    31861 cgggacaaca cgatctccag gtgaagctct cccatgccgc tgatgatggt ctgccccgtg
    31921 tccggattct cctggtgctt gaaggtgggg tcctcctcgg atagcttggc caggctgtcc
    31981 aggagctttt cctggtcccc ccgggtcttg gcctccacgg cgatggagat caccggctca
    32041 tacgtgccga tgggctcgag gagaatcggg cgggcaggat cgcagatgga atctcccgtg
    32101 gaacaggact ttagccccat gaccgccacg atgtccccgg cccccgtctt ttcgatccgt
    32161 tcccgcttgt tggcatgcat ttgaaaaatg cgggagatct tttcccggct tcctttggag
    32221 gcattcaaca cgtccatgcc cacctgcagc tttcccgagt aaacccgcag gtacgtcagt
    32281 ttgcgcccct ggtccatgaa gatcttaaaa gcaagggccg ccagcggatc ctggtcgctg
    32341 ctcagtcgat gctcgttttg tcgagtgacc gggttcatcc cttccacggg aggcacgtcc
    32401 aggggagagg gcagaaagtg caccacagcg tccaggaggg gctggattcc tttgttcctc
    32461 agggccgtcc cgcagagtac cggcacgatc ttcaactgca aggtgaggcg gcgaagaaga
    32521 gggatgaggt cggcggcgga gatctcttcc tcctggaggt agcgttccat aagctgatcg
    32581 tcccactcgg ccacccgagc caccagacgg tcccgggcat ccgtcacctc cccaagaatc
    32641 tccgggtcga tgtccacaaa ctcatattcc gaccccagcg tctcgtcccg ccacacgatc
    32701 tgtttcatct gcaccaggtc cactaccccc cggaagtccg cttcccgacc gacgggaaac
    32761 tgcagaggaa gcgcaaccgt tcggaacttc tgctcgatct gctcgatcac ccgctggaaa
    32821 ttggccccca cccgatccat cttgttcacg aaagcgatct tgggaacatg gtacctgtcg
    32881 gcctggtgcc acaccgtttc cgactggggt tccaccccgc ccaccccgca gaacaccgcg
    32941 acggccccgt ccaagacccg cagggatcgc tccacctcga tggtgaaatc aacatggccc
    33001 ggagtgtcga tgagatggat ttcatgatcg cgccagtgac aggtggtgac cgccgaagtg
    33061 atggtgatcc cgcgctcttg ttcctgttcc atccaatcca gaacggcggt cccgtcgtgc
    33121 acctccccca tcttatggga ccttcccgtg taaaacagga tcctctcgct cacggtggtt
    33181 ttccccgcat cgatgtgggc aatgatgccg atgtttcggt aggtcttgag ccgacgaacg
    33241 tcactcatac cggtgatcct ctcagccgta aaccctgtct gttcccatcc agcccctcct
    33301 cggcgacacc ccgggacggc agtcctcttt cgttcccctc tgcttcttat cattgtttgg
    33361 agaggatagg taatgtaggg aattgaacgg aatgtgtcaa gtttgggtct tttcccgacc
    33421 gggaaacccc gggtgttgat ctgagttgag cgattccgag agcatccggg cgtcatgccc
    33481 gtcaaggcgg gcatccggca ggtatgcacg ctcgtttcgg attcccgcat gcgcgggaac
    33541 gacgaaaagg ggttcgtacc cgccaaatct ctccatttgg gggtgacttc acggcatcaa
    33601 accgttgcga aaatccttct gccggacccc ctgcgggggg gaagattcgg atcctctaac
    33661 ctattgaatt tacatgtcag ttcagaataa tttagatcta actcaaaaaa gcgccaaaaa
    33721 gcgagattag ccaaaaagct atgcactaga atgatttatg ccgctaatag ccgattcaga
    33781 ccagcccatt ttgtctgaat atgtttgcgc gtctttggat agctccctca ggctcaccaa
    33841 tgtcggaaga atttgcccgt aatccgggct aattcctcca aatcccgatt tatggagttt
    33901 gcctcggaca atcccggcct gctaaaaggg cggcatccga aaactcttac ggtgagaatc
    33961 cgcgagggcc atgattctct ttgaaatgag aagaaggagg accagtatgg cgccaaaggc
    34021 caagttctgg aggcaccaac tggggaaatc aaccatctgt cgtaggcacc cgctgctgta
    34081 cggcacgctt ccattatttc ttctgctcat cgcttgtttc accacccaaa tcgaggcaag
    34141 gacggaaccc gacacctgga gcgagctctc catttccttt ctcactcctg agaaaatctc
    34201 ccacgcaggc tcccagatgt tgacgtccct gtccccacct tttgcagtgt acaacatttc
    34261 cccagtagga aggtcgagga atcgggttcc catccacatt cccaacgaac cttccgttcg
    34321 caagtacatc cagtattacc ggaaagaggg ccgagcaacc ctcgccggca ccctgaaacg
    34381 ttcgtctcct tacgtgcccg tcatggccga gattttggag tcgaacgggg ttcccgcaga
    34441 gatgatcgcc atcgtgatgg tggagagttc ctttaaagcc cgtgccgcat atcgaggagc
    34501 ggcgggctat tggcaactcc tgccgggaac cgctcgatcc ctgggactca gggtggatcg
    34561 ctgggtggac gaaagagccg atcccatcaa atcaacccaa gccgctgcca agtacctgcg
    34621 ctccttctac gatcagtacc agtgctggac gttggcaatc gctgcctaca acacggggga
    34681 tgcccctgtg acccgggcag tgaagaaatg caacgccacc gacttctggg aactgtacag
    34741 gaagtccgcc gctcccagca ggagccggac gtatgttccc aaggtgctgg cagccatgca
    34801 cattctgagg aacctggaag cccacggctt cgagtatcct cgaaactttc ccgtatatga
    34861 ctttgaatcc atttgggtca actctccttt gaaactggaa caggtcgccc aatggatcaa
    34921 cgtgccgatc ggtcagatac gggatttgaa ctcgtctctg gtgcatgacc gtgtgccccc
    34981 cgaaaacggg tttgcactcc gattgccctc gggaactcga gacaagttcg acctggcgta
    35041 tgaggattac ctgaaaaggt aaacactctg aggaaggaac cggccccgcg gatcgtgccg
    35101 gacggcatga aaccaatggg ttcacgcgga tgagtattcg gatgttggcc actgagctct
    35161 accgtgcgat gagaacagtg gaggagatca aaaagaaact cgaggaaaca ggtcttcatc
    35221 cctcgattcg gggtgaactg gaagaccagc tcagagttgc acgggctgaa agagatcgga
    35281 tcaaggctct catggagggg gcaaaggcat cgtgaacatt cgctcacggg cctttgccct
    35341 cttctttttc cagaatcccc tccgaagtca tcgaaaagcc gccgcacgcc tcttcggaca
    35401 aggacccacg ccggaccaac cggttcgccc tcgggggctg catgccgtgc caaggccttt
    35461 caattcgccg cacgcggaac agggaccaca ggtgcacctg accacaaagc gacggtccgc
    35521 cgatctcaaa gcaaccggtt catcgtgcgg aagaattccc cgttgcccac cgccatcggg
    35581 tcgggtttgg ggtagggact cgaagcgcga ggcattgccc ccgttgaccg atgtcgaaag
    35641 aaggccggag cagtgaggaa tccaggaata cggcgaaggg ccccggagtt cccggatgag
    35701 gcggaaagaa cgttcgagga tctctctcct gcggggattc atgaaattga agggcgaagg
    35761 atggaaggtt gtgaggatcc cccgtatcct gggccatcac acagcttgcc gcaaacgtct
    35821 ttcatccggg caggcgggtg aatccgggag aacccgggtg acggccgtcc ccaggcacac
    35881 cacgccttgg gcccgagaat gtccagttct ctcctgaggg ggttcgatac cgagcatctt
    35941 cccggatctc cccgtagagg tcttcgagca gtgtgcatgg gttcggatcc gccttcaata
    36001 ctcgatcccc ctctgggcct tgaggccccg atcatgatac ggatgcttca ggggcaccat
    36061 ttccgtcgcc aggtcggcca gcgagatcag ggctttgggg gcgtttctcc ccgtcaccac
    36121 tacgtggagc cccgggggcc gatgctccag gacttccagg acctcctccc agggcaggag
    36181 cccgtaggag aggacgtagt tcaactcgtc cagaacgatc aagccgtagg cgccgcccgc
    36241 gatggcctct ttggccttct cccacccgga tcgagcaagc tgccggtctt cctcgaggct
    36301 ttccttctgc caggtgaaac ccgtccccaa gggcaggatc tcgatgcagt ccagtttctg
    36361 cacggcatga agctccccat aggaccggga ccccttgatg aattgaagaa agagaacccg
    36421 catcccatgt cctgctgccc gcagggccat gcccagagct gcggtggtct ttcccttgcc
    36481 gttcccggtg aacaccacca ggagtccctc ttccatcctc tcctccccca gaggcattcc
    36541 gcgaaatgcg ggtgatctta cccccgaacc ccgcctatgg aaaatcgaaa aaaaagcggg
    36601 cactgcggcc cgcaattgaa tcttcctgtt gccggttccc gtggccccac catcgaactg
    36661 gctcggttaa ggggcctatt ccccgggaag acttcgcctg ttcctagtcc gcggcagtct
    36721 tctcgccggt cgatgccttg gtttccttct tggttgcttc tcccttggac gccgaacaac
    36781 tctggttctt tccggaatag tccgttacgt accagcccga acctctgagg tggaaagtac
    36841 tcatggagat cagcttgtgg agctcgccct tacagtggct gcattgtgta agaggcgggt
    36901 ccgaaaactt ttgcatggcc tcgagggtct tcccacactt caagcattcg tactcgtaga
    36961 ttggcatacc agaaacctcc ttcatctaga ctttcatcac gaggcatgat cgctcgggga
    37021 aaaactcagg ttccattata aaaccccggc atcctttgtc aagatcccat tgcccaacgg
    37081 aggaagcggc ggcggggcct cggggaagga ctcccccccg cccttgcgtc ccaagggttg
    37141 caggccccgg aaatacccgg ggttgctctc ggggcccccc cgctcgctcg gtcctttgcg
    37201 aggccggact tacggggcta cctggaccag ctgtccgccg gatacctgga ggatcaggta
    37261 gcttcgttcc acgttccccc cgggggtgaa gaaatagctt ccggcgattc cttcgtaacc
    37321 ccgaatctga agcaggttct gcagcaaacc ggcccgatcc atgttcgaag ccccccctgc
    37381 gtatcgcgcc tgcaggaaca gcatcatggc gtcgtaggcc tgggcctcca gataagaagg
    37441 ggcagccccg taaaatcgtt ggtatttctc ccgaaaggct cgaacttgcg gagacgcagc
    37501 ttcggcaaag aagggggtcg caaagagggc ctgttccacg taagcccctc ccgcttgaac
    37561 caggggggcc tctccccaca gattggttcc caacagggtt gccccaacga cgttgttgta
    37621 gggaagctgc ggagcaatga gggtcaccgt ctggacctga tccgggatga acagtgcctc
    37681 gaagggggtc acatccactc ccgtgggagg gacgttctgc ttggccaggt tcatgagctt
    37741 ctgaatgggc tctttgaaat ccgtactccg atccttgtag ggcacgctgg ccaaaagatt
    37801 gcctccctgc tcctgaacca cctccgaaaa aaccttggag agtcgcaggc cgtatcgatc
    37861 gtccgggtag aggaccgcga atcgggtgta tcccagtttt tccctgcagt acttgacaag
    37921 ggtttggacc aagtcgtgat tgtccagaaa aacgtggagc acgaaggagt ggtccgcgga
    37981 ttcctcgtct ctttgggtca aggccaggag cggaactccc caccggttgg cgatggggga
    38041 aacctctttg gcggactgga tgcccaaggg gccgatgacc gcaaggacct ggtccccttc
    38101 gaccagatct tcgaaggcct tgccggaccg gcccgattcg gcgggggcgt cttttaccac
    38161 cagggtaacc ggcttgtcgg ggtggccggc cttccagtct tccatggcca tggagaggcc
    38221 ccgcaacacc agacggctgt atttttcata ctgtccgttg aggggaagga tacacccgac
    38281 ccgttcggca cggaccccga tctccacctc agcaggggcg ccggtcccag ggcccccccg
    38341 gagggattcc cggatctcgg aagccagggg gtgcgtaggg ttctgcttca ggagctcccg
    38401 caaccgctct ttgccggctt cggcatctcc tttccgggag tccagctgga cgagccgata
    38461 ttccaggaat acccgcatga agctcgagga ctccttcttc atcaagcggg ttactttcga
    38521 ttcattggcg tctttgagca gatccagggt cggtttgacc aggccctctt gttccgcagg
    38581 ggtcttggct gcggcaaacc ccgctgcggc ccattcgaaa cccgtctcga catccccctc
    38641 ctccgccagg attttcacca tgagctcgct gagtcccctc cggacttcgg gctgtcccga
    38701 cgaagcatcc cacagggaag tcacttcctt catcatctgc ttcttatggc ccgcggcagc
    38761 catggccctg aaccgatcca accgggcagc attgattccc tcccagccgg gaaaagcata
    38821 cacgaggtag tccaggtact gaagggcccg gtcgggttgt cccatttgga gatagatctg
    38881 gcccacccgg tgaaatcctt tggcggccaa agccgtatcg gggtaagtct gaatgatcct
    38941 ctcccacatg gaaatggccg ccgccgtttt ccctgctttg tactcctgtt cggccttccc
    39001 ggccaaagcc tccgcaccgg gaggggccgg ggacagagtc ctcaccgggg cactcgtggg
    39061 aggcgattga gacacagggg cacagctcga aagaaacaga acgaccgtaa agatcgagaa
    39121 ccaggggctt tgtcgcatca taggactcat attctttgaa ccggcagctg ggagtcgata
    39181 cgtccgaagc agttttcctt taaaaacaag ggcacacgaa aaatcgtgtg cccttttatg
    39241 ctggaagtag cggagtccgt caatggaaaa gcaccaatta cttgtccttg acttcttcga
    39301 aatccgcgtc cacaacatca tcttcgggct tcctgccgga agacgcagcc ccctcgggac
    39361 ccccaccgga ctgggccgtc tcttgttgct gtgccgacgc ccgcttgtag gcctcttcgg
    39421 ccaccttgtg ggagatttgc atgagctggt ccgtttccct ctggattgta tctcgatccg
    39481 tcccctccat ggctttcttg agggtctcga tctgggactc gatgctctgc cgagtggccg
    39541 gatccacctt gtcgcccatt tcccgcatgg tcttttcggt gctgtaaatg agggtgtccg
    39601 cctggttcct tgcctcgatg agctcccgct tcttgcggtc ctcttcggca aaggtctcgg
    39661 cttccttcac cagcctctgg atttcttcct gggaaaggcc gctggacgcc gtgatctgaa
    39721 tggactgttc tttctgggtc gccaggtctt tggcggacac gtgcacgatg ccgttggcgt
    39781 cgatgtcgaa ggtcacctcg atct
//
LOCUS       DAOOVC010000020        39608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_657_length_39608_cov_5.996283, whole genome shotgun sequence.
ACCESSION   DAOOVC010000020 DAOOVC010000000
VERSION     DAOOVC010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 39608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39608
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_657_length_39608_cov_5.996283"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PLM79_04570"
     CDS             complement(<1..1060)
                     /locus_tag="PLM79_04570"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOV85612.1"
                     /translation="MRKSLPCLLLWFMMCGLLASSSAWAAEPVGNVESIVGTVQVERE
                     GRTLPAKAGDPVYLLDKWQTRAQSSAEIVFVDNSRVKLAAATVLEITEYVYNPREQKR
                     ESLFSMLVGKARFLVQDLQGYKEKRFRVQTQTAIVGTRDTDFVVGVVPEPPVGGVCRE
                     PFVEAYCVENAIIMASRNRPDKPVILTSNMISQACGSELPTPPRFATAAERARMIRGV
                     EKIGGKTEIGPARPKAAAAAKGEDKSGAAVGTGDTDGLGPRGGDGSGAGGVGPLGGST
                     TTTTLDTTTTTTTTTTTTTTTTTRPTTTTTRPTTTTSTAPTTTTTTTAPTTTTTTRPT
                     TTTTTAPTTTTTLPPPPPP"
     gene            complement(1072..2622)
                     /locus_tag="PLM79_04575"
     CDS             complement(1072..2622)
                     /locus_tag="PLM79_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV85613.1"
                     /translation="MMIGSHCLRYWLAFMSVLVGLAPCLAQVLPPPSPEVSISHDLAV
                     QALRARNYEEALSQVQTAILQDPINLDYQYTLASVYIHLNRPDEAEGILLALLREDER
                     SFQKAYFDLAEIYLQRNEDTRAAEMLRKARPLDPGRADYQLGLIEMRRKQYAGAIPLF
                     RSAAATKPELAPYALTQQGIAEYHLKNFKEAKTTIQKVLTMKLTPEETAQLRQLLQAI
                     EGAARAAKPWYLSASLGVQYDDNVLLNPSGQVRLAPGTRNDKDEDDVGFLTSIYGRYN
                     VVEKDPWKLGVAYNNYSLTYVDHTDLSVLGFRPSIYAQWDKAPVSAYMEYVYSHYLVD
                     NESKVDVHSIFPRVAMMHGKRWRTEAFGGLEWRLYEDQNPDSRHYFLGLTEMFFLMDG
                     RAHVRGGYLFRYEDFVPREVGNVLSNEAMLGFQAPVWKNKWFLDVAGRYIWREFDHDP
                     AISRKKDRCDDEQNLSVVLYGQLTPCLQLSLIYQHTWNDSNIWSYQSFDPYDFQRAVF
                     TCMLTFSY"
     gene            complement(3371..3700)
                     /locus_tag="PLM79_04580"
     CDS             complement(3371..3700)
                     /locus_tag="PLM79_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOV85614.1"
                     /translation="MEAKDEVPREKLVVMETGEGIAFQVLVQPRAARNELAGLQQGAL
                     KIRLTAPPVEGEANDACIRYVAGLLGVPRSSVDILRGRTSRKKTLRVRGLPPQAVQKL
                     LTDSPPS"
     gene            3923..5659
                     /locus_tag="PLM79_04585"
     CDS             3923..5659
                     /locus_tag="PLM79_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV85615.1"
                     /translation="MEKVLRIDMGADGGPAFREEPLGDYAGLGGRGLTSAIVCKEVDP
                     LCHPLSSQNKLVIAPGLLGGTTAANSGRISVGAKSPLTGGIKEANAGGQAAQALGRLG
                     YAAVVIEGKPQTDAIHKIFIGKDGVRISEEPQLKMLPNYDLVEVLRREHGDGISCISI
                     GPAGEMKLCAASIACTDMELRPTRHAGRGGLGAVMGSKGVKVLVLDDSGTKPRAPRDP
                     EGFKAANKAFVEGLRAHPVTGQALPAYGTNVLTNILNEAGGYPTCNFSRGRFESAHRI
                     SGETMAETETARGGMTAHGCHRGCVIRCSGVYHDKDGRFLSKQPEYETVWAHGGHCGI
                     CDLDAIAMLDFLDDNFGLDTIEMGVTIGVAMQAGLIPFGDAQGAVRLVEEVGRGTPLG
                     RILGSGAAVTGKVFGVEKVPVVKGQALPAYDPRAVKGVGVTYATSPMGADHTAGYAVT
                     ANILKVGGDVNPLRSQGQVELSRDLQIATAAIDSTGMCLFIAFAVLDRPETFQALLDM
                     IRTLHGIPLTAEDVVEMGKKILKMERDFNLRAGFSSAHDRLPRFFRDEPIEPHGVRFD
                     VPEEELDQVFNW"
     gene            5910..6821
                     /locus_tag="PLM79_04590"
     CDS             5910..6821
                     /locus_tag="PLM79_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV85616.1"
                     /translation="MQPIIEARDLRKTFADFVAVDGIHFRMMPGECLGLLGPNGAGKT
                     TTIRMIYGFSPITRGSLRVFGLDIRKEWRTIKGRMGVCQQENNPDPDLSLMENLEVFA
                     LYFDIPRKEARRRARDLLRFMGLEHRMDAKVTELSGGMMRRLILARSLLNNPDLLILD
                     EPTTGLDPQSRHQVWERLEELRSRGLSVLLTTHYMDEAARLCDRLILMDHGKILIEGK
                     PSRLIADHVGRDVIEMVNPDPEVRRWVQSSGFRHDDLERRLIVYGKEGENLFDRVGKR
                     YCSEECVFRMATLEDVFLRLTGRELRE"
     gene            6818..7609
                     /locus_tag="PLM79_04595"
     CDS             6818..7609
                     /locus_tag="PLM79_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV85617.1"
                     /translation="MRGIGPGTVSRRFFRVWQRNFLVYRRIWKINFLPPLLEPFFYLL
                     AFGVGLSGLVGNVSYGGKELSYAAFITPALLAINMMQNAFFENTYASFVRMYYQKTFD
                     AMMATPLSLQEVILAEIIWGATKSFFATGVMLGVVSLFGLVHYPHGLLILPLSFLAGI
                     AFGSMGMLFTGIVPSIDLFNLPVFLFITPMFLFSGTFFPLDALPSWAQYVAMVLPLTH
                     LVEAIRRLCVGELPWNLLWGLGYLVLLCALFLPLSTAVMHRRLIH"
     gene            complement(7819..8229)
                     /locus_tag="PLM79_04600"
     CDS             complement(7819..8229)
                     /locus_tag="PLM79_04600"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOV85618.1"
                     /translation="MNEAVKLRLAEDAQDLFSSEERKAEPNSRSEDVLKDLTRRVKIQ
                     PLLKATSAVKETESIDSKGDRQRVVALIGRLADNPRPPGCEKLSAQDEYRVRQGSDRI
                     VYSIEDQDLVVYVVKVGHRKDVYRETAVFKGPLT"
     gene            complement(9400..10767)
                     /locus_tag="PLM79_04605"
     CDS             complement(9400..10767)
                     /locus_tag="PLM79_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOV85619.1"
                     /translation="MNRSGLFLDKKMRKTVIPGGWPGSRVVGWTLAWLVMSASAVFAE
                     EAPPAVNAGDTAWLLVSTALVMIMTPGLALFYGGMVRRKNVLATIMHSFIAFGVISIQ
                     WVLFGYSLSFGPDVGHVIGNLSWVGLRGVGLEPNADYAATVPHQAFMIFQMMFAVITP
                     ALISGAFAERMKFKSYLAFIVLWAIFVYDPLAHWVWGTGGWIRELGALDFAGGLVVHI
                     SSGVSALACAVVMGKRKGYGVDLMAPHNLTLTILGAALLWFGWFGFNGGSALTSGALA
                     TSAFVVTHIATAAAALSWMFVEWSHRGKPTALGVVSGAVAGLVAITPGSGFVGPMSSI
                     FIGLVAGGLCYIAVNMKARLGYDDSLDVVGVHGVGGTWGALATGLFASKLINPAGSDG
                     LFYGNAIQPAIQAASILTAWIYSFAVTWIILKVLDAVMGLRASEEDEAQGLDLSQHGE
                     TGYIL"
     gene            complement(10834..11172)
                     /locus_tag="PLM79_04610"
     CDS             complement(10834..11172)
                     /locus_tag="PLM79_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOV85620.1"
                     /translation="MIKIEAIVKPFRLNEIQDALLALGIQGMTVTEVKGFGRQKGHVE
                     IYRGSEYQVNFVPKILLMVVVPDELAEQVVDAIQKTAYTGKIGDGKIFLSRISEVMRI
                     RTKETGPEAL"
     gene            complement(11779..12910)
                     /gene="prfB"
                     /locus_tag="PLM79_04615"
     CDS             complement(join(11779..12837,12839..12910))
                     /gene="prfB"
                     /locus_tag="PLM79_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150109422.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOV85621.1"
                     /translation="MNIDIAEIKAGMKELSQRIQNLGGIFDIDGRKKRSAELEKQLLK
                     PDFWERPEQGKEVLKERSSLLEILEEWQKLLQEFEECDLLLSMALEENDEGTMEEVQQ
                     KLRGIRKSLRALELRQMLGDENDEHNAIVSINAGAGGTEAQDWAEMLLRMYLRWCERK
                     NYKTEMVDLLEGEEAGIKNATFTVTGRFAYGYLKAEIGIHRLVRISPYDASGRRHTSF
                     AAVFVYPELDDQIVIDIKSSDLRIDTYRASGAGGQHVNKTSSAVRITHLPTGIVVQCQ
                     NEKSQHRNRDIAMKILRSRLHERERQAQQDKMQEIHDNLGDIAWGNQIRSYVLQPYRL
                     VKDHRSNTERGDVESVLDGDLDEFIEAYLLSQVSSRERNVAA"
     gene            complement(13059..14585)
                     /gene="lnt"
                     /locus_tag="PLM79_04620"
     CDS             complement(13059..14585)
                     /gene="lnt"
                     /locus_tag="PLM79_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOV85622.1"
                     /translation="MWLAVLLSILSGILLTLGLPKATLPYLSWVALVPLLTALGGRSS
                     RESLGLGFLCGLVHFLTMLYWIRYVVDHYGGLPFPVAASVLLLLCLYLALYPALFALC
                     SHLWEDSPRLWVFGLPGVWVTLEWVRAHALSGFPWGNLGYSQTPFLTLIQAADITGVY
                     GLSWLLVLCSTTVVGCFRLPPVRKWAIPVFCLVLGCVLVYGSWRIRDVNGLQARVRPW
                     NAAVIQGNIDQSKKWDPAFQQETLARYARLSRGAAKNHPPPDLIVWPETATPFFYGVE
                     EQLTGQVNEIIAETDKPLLFGSPAVTRTGGEPGFQNRAYLVSREGRIEGAYAKRHLVP
                     FGEYVPFKRLLFFVHRLVEAAGDFSAGRNPEPIAFGEQQLGVLICYEGIFPYLARSAV
                     EHGATVLVNVTNDAWYGRTSAPYQHLEIARWRAVEFRRPLIRAANTGISTVFDAVGAA
                     CGQIPLDEEGWLVCPVRPLNIRTVYASWGDFFAWTCVASCAGCLLYTLPGKRRNRASS
                     "
     gene            complement(14653..15525)
                     /locus_tag="PLM79_04625"
     CDS             complement(14653..15525)
                     /locus_tag="PLM79_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOV85623.1"
                     /translation="MERISARGFQNWLRMVFRRLARVEDAQNIEKEIQQLIDEGEQQG
                     LISEDEGEMIQGIFSFRDTIAREIMVPRTDAVCAPLQTGISDIIQLILRTGHSRIPIY
                     KENIDNIIGTLHAKDLLSNWGEVDLDISGIVRPPYFIPETKKISEVLKDLRGNKSHMA
                     IVIDEYGGTAGILTLEDIIEEIIGEIMDEYDADQMLIAEHEDGSITVNARLDVEELED
                     YFDMELPEGKFESVGGFVISLMGKVPAVNEIVVYKDLEMRIEAASSRKIEKIRIRKLE
                     GEATATRSSGEVFS"
     gene            complement(15631..16548)
                     /gene="speB"
                     /locus_tag="PLM79_04630"
     CDS             complement(15631..16548)
                     /gene="speB"
                     /locus_tag="PLM79_04630"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOV85624.1"
                     /translation="MSEPMVGSRPPECQGPHSSFLGLNAEESRFDSANAVVVPVPYDG
                     TTTFRAGTREGPRAILNASRELEFFDDETLTEVHREGIATLEELPVVVSSPRDMVNQV
                     QRVGDFLLSAGKLPVLLGGEHLISLGMIEAAVGHFPDLTVLHLDAHTDLREEYQGSAF
                     SNACVMRPVSRLCSLVQVGIRAVTGEELQWVRDRKIPCFFARDLWRDPSLWEEIPSKL
                     GPHVYISIDLDVFDPSIMPAVGTPEPGGVGWYEVLSCLRRVVQQHRVVGFDVMELLPI
                     PSMTAPDFLAARLVHKVLSYIFLDNPPRA"
     gene            complement(16559..17005)
                     /gene="speD"
                     /locus_tag="PLM79_04635"
     CDS             complement(16559..17005)
                     /gene="speD"
                     /locus_tag="PLM79_04635"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOV85625.1"
                     /translation="MGNKPEFGFGVHLMVDGYGCDRAGLEDINGIYGFLDEYPARMDM
                     TKIMPPYVFRYTGSTPEDWGISGFVLIAESHISIHTFPEKQYLSLDMFSCKPFDTDLA
                     VETVKRYFGMQKCEIKVLDRGQEFPNSIRASAQVVRMDRMLMQRSK"
     gene            17065..17496
                     /locus_tag="PLM79_04640"
     CDS             17065..17496
                     /locus_tag="PLM79_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85626.1"
                     /translation="MFTALRLEMPPQKKALQGLKRLQLLTPRWTLHETLRAVLPETKY
                     QRWDQDQKTVAPSETWIWFSAKSRCDGMGSPPPLFLVFKELSKAYPVSAARPPRCALY
                     KGPPMECQEKMIPGKDSKKLEKMKKAGYFPEDPHQENYSDD"
     gene            complement(17493..18416)
                     /locus_tag="PLM79_04645"
     CDS             complement(17493..18416)
                     /locus_tag="PLM79_04645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOV85627.1"
                     /translation="MEPNSENYKVPGQSILVVDDEEQVCELLAEALTYMGHQVVTAAD
                     GQDALEKLEHADFQIVITDLDMPRMDGMELIRYLVQNRNGTDIIAITGHAMKYRYTDV
                     VAAGAADFISKPFSLDELEAKVKRVIRERELRTELERLAIHDPLTGLYNRHFFARIVR
                     REAVRSIRYEHALFLFYLDVDRFKEYNDKHGHQAGDQLLIQLSKALKASVRQDVDTAF
                     RFGGDEFTVLLPHLDRDQAGMVAERIRQNYNSLGLEPTHLSIGMARFRAKSGDVDLDV
                     EDMIHRADGALYRAKHDLGGNTACLDKDSLF"
     gene            18565..19437
                     /locus_tag="PLM79_04650"
     CDS             18565..19437
                     /locus_tag="PLM79_04650"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOV85628.1"
                     /translation="MQCVAIVYKRSRPEAAKLARIIKDWLTSRHVEVLCQENVDQQGR
                     SCASSPKLEIPPQAQTVIVLGGDGTLLSVARFLAGRTTPVMGINLGGLGFLTEINMED
                     CFRELKEILGNNYEIEERMRLEVTIQRDGQEVIRQSVLNDAVINKGALARIIDIKTSI
                     DGKYLTNYRADGLIVATPTGSTAYNLSAGGPIVYPTARVVVLTPICPFTLTHRPIILP
                     SEVVIQVEIVGPARDLTLTCDGQVGCELSPLDRIRISTQASPLRLIKTPSINYFEILR
                     TKLKWGQAELRPLP"
     gene            complement(19461..20339)
                     /locus_tag="PLM79_04655"
     CDS             complement(19461..20339)
                     /locus_tag="PLM79_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV85629.1"
                     /translation="MSGMHYEGMIIRPPSEADSILLQVTVGCSHNQCSFCGAYKEKRF
                     RIKDPEIIDQDIDYAAANYSFLHRVFLLDGDALIVPQPRLVGILERIREKMPWIRRVG
                     VYGNAKSILRKTPEELNALRELGLGIVYLGLESGDAQVLEDIRKGVSPERMVEAGVRV
                     RRAGIKLSVTVILGIAGLERSRIHAKATGEALSAMDPNYVGALTLMVLPNTETAQRVS
                     RGIFRVPEPRELLAELREMLLHTHMTRGLFLANHASNYLPLRVRMPSEKAKALAMVSE
                     AVEGGRALRPEWLRAL"
     gene            20509..21486
                     /locus_tag="PLM79_04660"
     CDS             20509..21486
                     /locus_tag="PLM79_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85630.1"
                     /translation="MHSLSSPLPPEAKCTRCRSRATIRLSSHHALFCPECFLVFFHRA
                     VSRAMKEFGVSAATPIMIAVSGGKDSLALWDVLHQLGYPVEGLHINLGIHDFSRASSG
                     AVDRFARSRGLSWREVFLEEIFGYAIPEIRRRTRRKICSVCGLLKRQLLNRLVVREGY
                     AVLATGHNLDDEAGRLLGNLVRHRRQYLEKLSPFLPSPHPRLPIKMKPLYRLEAGEIR
                     LYCQIRGIEPVDSLCPLSRGATSHAFKEALQFLEDKMGGTKRDFLFGYLDRPAREASM
                     PGFGTCARCGEPTYSDLCSVCGLKSRLDGKKGKETDAVPNIPAPQIKGS"
     gene            complement(21473..21976)
                     /locus_tag="PLM79_04665"
     CDS             complement(21473..21976)
                     /locus_tag="PLM79_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV85631.1"
                     /translation="MNFREKVRAGDREWVREVVESSGFFTDRETEVAVELVDEALQRG
                     EKSGYYFLFAEETEGILGYTCFGPIACTRSSFDLYWIAVRPEVRGRGLGRELLKRTEA
                     LIEAKGGTRVYVETSSQPLYEPTRAFYEHNGYGKEALIRDFYAPGDHKILYGKVLKGS
                     GLSPNSL"
     gene            complement(22360..23364)
                     /locus_tag="PLM79_04670"
     CDS             complement(22360..23364)
                     /locus_tag="PLM79_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOV85632.1"
                     /translation="MRIAVLYNELSQPATPDEEDVLVQVEAVSSALSELGHEAVAISF
                     SLDLRKSLASLGDLRPEMLFNLVEGLDGSGRLIHLAPSLLDHLGIPYTGSSAEAIFLT
                     SNKPIAKTWMQASKIPTPPWYSGRPGEARLPFSPGQYIVKSVWEHASVGIEEDSVVEV
                     GDWPELCRQMSRRRGILGGDCFAEAYIEGREFNLSMLAGPSGMKVLPAAEIRFDDYPR
                     HRTRLVGYRAKWDAESFEYAHTPRSFQFAEADAPLICRLKRIASRCWEAFGLNGYARV
                     DFRVDPVGNPWVLEVNANPCLSPDAGFFAAAAESGLSYPQMIRRILEACPAPDGVPGE
                     "
     gene            complement(23377..24423)
                     /locus_tag="PLM79_04675"
     CDS             complement(23377..24423)
                     /locus_tag="PLM79_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOV85633.1"
                     /translation="MRVIGLTYDLRRDYLAMGYGEEETAEFDHPDTIDAIERVLRDLG
                     FGTDRIGHGKDLTARLARGDRWDLVFNLAEGLHGYGREALVPAILDAYEIPYTFSDPL
                     VLSLTLHKGLAKHVVRDMGIPTPDFAVLESHEEARSIALPYPLFVKPVAEGTGKGVTP
                     ASRVRSPEELGVVSRRLIETYRQPVLVETFLPGREFTVGILGTGKAAEAVGVLEVHLN
                     DRAEQEVYSYVNKERCEELVEYRVATDPEALRALALALEVWRGLGCRDGGRVDLRSDS
                     RGKPHFLEVNPLAGLHPEHSDLPILCSRVGISYPGLIEKIVRSALDRASNSPCPGTRK
                     ECRLESRDPGRIPA"
     gene            complement(24420..25625)
                     /locus_tag="PLM79_04680"
     CDS             complement(24420..25625)
                     /locus_tag="PLM79_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HOV85634.1"
                     /translation="MSARSDAFRKQYFPKATLADWNDWHWQLRHRIQDPEFLGRVLDL
                     SADEIEALGRSSTSLPLSVTPYYASLLDREDPAHSLRRAVVPDRREHLRHPSEAEDPL
                     DEDRDSPVPGIVHRYPDRVLFLVTGFCSTYCRYCTRSRMVGDRQRNLHMGSIRQWERA
                     LDYIRATPAVRDVLLSGGDPLTLSNERLEWLLSSLRSIDHVEFVRIGTKVPVVLPQRI
                     NGLLTRMLRHYHPLWMSIHFTHPDELTPEVSCACNRLADAGIPLGSQTVLLAGINDRV
                     DTMKRLMQGLLRMRVRPYYLYQCDPIPGSLHFRTPVSRGLEIVQGLRGHTTGYAVPSY
                     VIDAPGGGGKIPLLPEYAVGRENGSLLLRNYRGDLYRYPEEAEAGSTSPDKGPDEFPA
                     CLCKEGIGP"
     gene            complement(25951..26241)
                     /locus_tag="PLM79_04685"
     CDS             complement(25951..26241)
                     /locus_tag="PLM79_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85635.1"
                     /translation="MKRDRRWMGLILCAVVIALVSFVWVNRAMAADEETVVGMVIKTE
                     RGIIIEADDGDYLVKGKDLSKMVGKMVEATGVITEGGKGETIEVKSVEEIKE"
     gene            26779..27483
                     /locus_tag="PLM79_04690"
     CDS             26779..27483
                     /locus_tag="PLM79_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HOV85636.1"
                     /translation="MRKLIVRVVKKLRRVLAPLVVPAFHILWYHDRATWKKNTFLGYR
                     IRQCPLDLHLYQELIHRTRPRFVLQTGVDHGGSILYFATLLDLIGAPPESLVVGIDIA
                     LSKDALKLDHPRIRLLEGSSADPDIVSRAKGLLPRDGGFVVLDSDHRKEHVLSEMELY
                     ADLVSVGSYLVVEDTNLNAHPVYPDFGPGPYEAVRDFLRRRRDFVRDDDVWKRNRFSF
                     HQGGWLKRVRPHRAGS"
     gene            28046..28894
                     /locus_tag="PLM79_04695"
     CDS             28046..28894
                     /locus_tag="PLM79_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85637.1"
                     /translation="MISAILYLFRAPRLTAIARSAEAMNVFAVMTAWLFPLIHHGRVW
                     VVYWILPYPNQGNLWPNFQSPLVFDVPAVSTYLTVSLLFRYTGMIPDLAVVRDRSTGV
                     RRKSYGFFIPNWSNTSQQWRHHGAAYLFFAALATPLVVSVHSVVSWDFAFPVVPGRHS
                     TIFAPYSVAGAIHSGLAMVITLFIPLRRIYRVEHFITVHILEQLAKTLLPTGLIVGYA
                     YVVEFFMAWYSGSATEYDLFLWRAFGNYRVTFWIMVLCNTVLPSVFFFKKARTGLPWL
                     FRSPWR"
     gene            29261..30931
                     /locus_tag="PLM79_04700"
     CDS             29261..30931
                     /locus_tag="PLM79_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOV85638.1"
                     /translation="MDIHPIQQLLRSKGRFREILGVFFRYGLADWLTTLPGGLLLEYF
                     PGMDPNKIRELSTAERIRMALTELGTTYIKLGQILSTRPDLVGPEIAGELAKLQSTTP
                     ADPPQVVREILEAELGASPEALYVRFDPVATASASIGQIHRAQLHDGTSVVVKVQHRG
                     IEDRIRQDVEMALLLAETAERYSPPLRGYHPVATTREFTRTLLAELDFGRELQNLLRF
                     HQRFRHDRGIRIPTPYPALSSRRILTMDRLEGVDLAHVEDLRGAGYDLDEFARRGARL
                     FLDMIFRDGFYHADPHPGNLLILPDGRLGLLDCGMVGRIDPRLREHIEDMLIAIAEED
                     SAQVCDLVVRLGALPAGFDAEALRNDIDLFIGEYGYQSLEQFDLTGALNDLISIIRRH
                     KILLPSRLALLFKVLIMLEGTARKLSPRFSLAELVQPYQADIVQRRLAPQRMFRYVRH
                     LSQDWLRLAEQSPKDLSGILEKLRTGRFDVRIDHRRLDSIMNRLVKAILAAALFLGST
                     ELLSSRIPPLAFGVSVPGMVGCGLALFLGIRVLWAILRSGKTTSDDGL"
     gene            complement(30972..31301)
                     /locus_tag="PLM79_04705"
     CDS             complement(30972..31301)
                     /locus_tag="PLM79_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85639.1"
                     /translation="METGNEHISIDITRLRMERRPLWMTPLGISSFAEILGDGGDLYT
                     DRLTPDFPRFNGQELMGDEEEEAKRCEGKLSEMEGFPPGSELTNTVEEAFRSVCFPEE
                     TEKILVL"
     gene            complement(31456..31881)
                     /locus_tag="PLM79_04710"
     CDS             complement(31456..31881)
                     /locus_tag="PLM79_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85640.1"
                     /translation="MKYYKQSHRWPHGYLLALALGGICLGAAAFAREDLSIRFELLVC
                     GVFALIAAAVLWKGRRHYLEIDGEWIIHCGFQVWKLKRADLLRVEHGRKGWADDNEPF
                     LTVHARGREYPVDGGFLVSEERIEELIRSMHGLGESPRT"
     gene            complement(32104..34089)
                     /gene="acs"
                     /locus_tag="PLM79_04715"
     CDS             complement(32104..34089)
                     /gene="acs"
                     /locus_tag="PLM79_04715"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOV85641.1"
                     /translation="MENPTGSILREGGGKEEEIYYPPSDVVENANLSRYMKQKGFSTW
                     EELYRWSCENFEEYWEEMARRLEWYRPWDQVLDDSGKPFYRWFTGARCNIVHNAIDRH
                     LKTWRRNKQALIWEGEDGSHRTFSYLAVNREVSKLANVLKGMGVARGDIVSIYMPRVP
                     ELIFSMLACAKIGAVHSVIYGGFSVEAVRERVGDAQSKVLLTADGGFMRGKIVELKRI
                     ADEAMDHSPSIQTCIVLRRTGHAVNMQAGRDYWLHDLLTLPIASTKCETVPLDSETPA
                     FILYTSGSTGKPKGVVHVHGGYMVGISATFHMTFDLKEEDRYWCAADPGWITGHSYIV
                     YAPFIEGATSFIYEGAPNYPYPNRWWQCVERYGINILYTAPTAIRGLMRFGNAWPSRY
                     DLSTLRLLGSVGEPINPEAWRWFHKVIGGGRCPLMDTWWQTETGMFMIAPNPTTPLKP
                     GSGTYPVLGVVADVVDDRHAAKPVDEDGFLVIRKPWPAMMRTLLNDPERYVQQYWRGE
                     QSEVYFTGDSARKDAQGYFWVIGRVDDVIKVSGYRLGTAEIESALVSHRAVAEAAAIG
                     LPHELKGSAIHCYVILSQGIEPSEGLEDELKAHVASEMGPIAKPERIQFVSSLPKTRS
                     GKILRRVLKAKALGQELGDLSTLEEQLSLQQLSMLNR"
     gene            complement(34849..35898)
                     /locus_tag="PLM79_04720"
     CDS             complement(34849..35898)
                     /locus_tag="PLM79_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85642.1"
                     /translation="MEQGLGRQLLDQFLREHWISGLFLTVTLTTVGVLLCARWWLRRR
                     WKKILEESREDQELDLLTPLGPKDREALEIIRSLRREVWEWPEGRLQLSVEALTALAT
                     RIIQSIAAVYHGTAGTPEYEASLVELLQLMRRVGLRLSRLTAIAPFRFFGSRRISDYQ
                     RYYQVYRRINDHPILRVVRRNPVLYRLARWAVHIKNLGNPFYWAGREVTREGYFFTLR
                     WFHLAFISQVGREAMRVYSGRSFQKEEDRDAALVCYRLFALTRRWGGPTAEEWAALVD
                     FVATQVDMENEVRLQVLSRMARGRLPAGLESQKLQTSSGQKWYRQGLKGLLEKDSGFN
                     PIKKQLLVLELSDVQ"
     gene            complement(36222..37385)
                     /gene="rdgC"
                     /locus_tag="PLM79_04725"
     CDS             complement(36222..37385)
                     /gene="rdgC"
                     /locus_tag="PLM79_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOV85643.1"
                     /translation="MPIQSSSASFVRYLLPDPLPGDLWSYVEEKLNAGRFRECEEGLE
                     WSAGFTSWDDFFEDSFEYSTHHKGEYVAFHFRVDQKKVPAMVLKQHMREAIRKHRDEN
                     GGKWPSRHQRQEMLETTQAMLLSRAFPKPGGFDVVWSPSARWMLLGTTSSKMLEIFME
                     HFEKHFRLYPLPLFHVNWASHLIELDERRKDSLHSMTSGGIRALEEGRFLGYEFLTWL
                     WFFIEQPHELLGLPQDRKAEIHLGERMILSLPQGGKERIVCTTQANALHEARTALRQG
                     KRVEQIQLFLRAGDNEYLFTLDSTLWSIKGLKTPKQPPAAREEDADGRFLERMYFLEE
                     VFSALNLLYERFLRERLDSSWESGTLKLMQHWIETGKEPEDGATVSTGQDAPF"
     gene            complement(37646..38101)
                     /locus_tag="PLM79_04730"
     CDS             complement(37646..38101)
                     /locus_tag="PLM79_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOV85644.1"
                     /translation="MEAREFIAVNEVFIKTDRIQGNMVILKESEDSAEYFMMFVGDAE
                     ITAIAKEKGLVEPKRPLTHDLYLGILRKAGVVFDHIEIFSMQENTYYAKVYVRLQGEE
                     LIFDSRPSDAVALALHEKIPILVNRKLLRRELSQEEIQEYEMIVKTVKF"
     gene            38336..38716
                     /locus_tag="PLM79_04735"
     CDS             38336..38716
                     /locus_tag="PLM79_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOV85645.1"
                     /translation="MKVCFPTETLQGLQSTVYGHFGSAPGFVIVETDSMTFEQIANAD
                     LHHAHGMCQPLRALQGRKVDGVVVGGIGMGALMKLNAQGVAVYRAMQGTVQENLDLIL
                     AGKLPSFTTDHTCAGHGGGGGCAH"
     gene            38759..39004
                     /locus_tag="PLM79_04740"
     CDS             38759..39004
                     /locus_tag="PLM79_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV85646.1"
                     /translation="MPIYEYLCLDCGTPCEQLVLGLDEDVSCPSCKSTRLRKLLSASS
                     PASGPKGEGRLPSQTDTACCGSRPGASGCIPGSCCGK"
     gene            complement(39034..>39608)
                     /gene="cysS"
                     /locus_tag="PLM79_04745"
     CDS             complement(39034..>39608)
                     /gene="cysS"
                     /locus_tag="PLM79_04745"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698491.1"
                     /note="catalyzes a two-step reaction; charges a cysteine
                     by linking its carboxyl group to the alpha-phosphate of
                     ATP then transfers the aminoacyl-adenylate to its tRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOV85647.1"
                     /translation="FSDESVGEAERGLDRLYGTLGAVLERSDPPGETVADEVLRKQDA
                     ELLEATATLPELFREAMDNDFNTAQAIGHLFGVQRHLQRFLDRFGRKKLKGPAAGLAR
                     RAGEALREHAQIFGILQRDPEVFLREQRSLKIRSTGMTEEEVEGLIRRRQQARQEKNF
                     EEGDRIRAQLEQKKIQLEDTPGGTRWRVLL"
BASE COUNT         7730 a        12272 c        11419 g         8187 t
ORIGIN      
        1 gtggtggcgg cggcggcggc aacgtggtgg tggtggtggg tgccgtggtc gtggtggtgg
       61 taggccgggt ggtggtcgtg gtcgtcggcg ccgtcgtagt cgtggtggtc gtcggcgccg
      121 tactggtcgt agtggtgggc cgggtggtgg tggtcgtggg ccgggtggtg gtggtcgtgg
      181 tggtggtggt cgtggtggtg gtggtcgtcg tggtgtccag agtggtcgtc gtggtggacc
      241 cacccaaagg tcccacgcct cccgcaccgc tcccgtcacc tccacgcggt cccaagccat
      301 ccgtatctcc ggttcccacc gcggcacccg acttatcctc cccctttgcc gctgcggcgg
      361 ccttgggtcg agccgggccg atttccgtct tgcctccgat cttttccact ccccggatca
      421 tcctagcccg ttccgccgcg gtggcgaagc gggggggcgt gggcagctcg gaaccgcatg
      481 cctggctgat catgttggac gtcagaatga cgggcttgtc cggtcgattc cggctggcca
      541 ttatgatggc gttctcgacg cagtaagcct ccacgaaagg ctcccgacag actcctccca
      601 cggggggttc cggcaccacc cccaccacaa aatccgtgtc ccgggtccct acgatggctg
      661 tctgcgtctg cacgcggaag cgcttttcct tatacccctg aaggtcctgg accaggaatc
      721 gcgcctttcc caccagcatg gagaagagac tttccctctt ctgctcccgg ggattgtaga
      781 cgtattccgt gatctcgagc acagtggccg cggccagctt tacccggctg ttgtccacaa
      841 aaacgatctc cgcggagctt tgagccctgg tctgccattt gtccaggaga tagaccgggt
      901 ctccggcctt cgccgggagc gtccgtccct ctctttccac ttgaaccgta cccacaatgg
      961 attcgacatt tcccaccggc tcggcggccc acgcactcga agacgccagg agcccgcaca
     1021 tcatgaacca gagcagcaag caaggtaagc tcttcctcat ggctgtcatc ctcaatagct
     1081 gaacgtaagc atgcatgtaa acactgccct ttggaaatca tacgggtcga aactttggta
     1141 actccatatg ttggaatcgt tccaggtgtg ctggtaaatc agggacagtt ggaggcaggg
     1201 ggtcaactgc ccgtagagaa ccacgctcag gttctgttca tcgtcgcacc ggtccttctt
     1261 gcggctgatg gccggatcgt gatcgaattc ccgccagatg tatcgcccgg caacgtccag
     1321 gaaccacttg ttcttccaga ccggcgcctg aaaccccagc atggcttcgt tgctgagaac
     1381 attgcccacc tcccggggga cgaaatcctc gtaccggaac aggtagcctc ccctcacgtg
     1441 ggccctcccg tccatcagga agaacatctc cgtcaacccc aaaaagtagt ggcggctgtc
     1501 ggggttctga tcctcgtaga gacgccattc gagtcctccg aaggcttccg tgcgccaccg
     1561 ctttccgtgc atcatggcga cccggggaaa gatggaatgc acatccactt tggactcgtt
     1621 gtccaccagg taatggctgt acacgtattc catatacgcg gacaccgggg ctttatccca
     1681 ttgggcgtaa atcgaaggac ggaaccccag aacactcagg tcggtgtggt ctacgtaggt
     1741 cagactgtag ttgttgtagg ccactcccag cttccacggg tccttctcca cgacgttgta
     1801 ccggccgtag atggaagtga gaaagcccac atcgtcttcg tctttatcgt tgcgggtccc
     1861 cggggccaac cttacctgcc ctgagggatt gagaagaaca ttgtcgtcat actgaacccc
     1921 cagagacgcg gacaggtacc agggtttggc cgcccgggcc gctccctcga tggcttgaag
     1981 cagttgcctg agctgtgccg tctcttccgg cgtcagcttc atggtcagca ccttttggat
     2041 ggtggtcttt gcctccttga agttcttgag gtgatattcc gcgatgcctt gttgggtcag
     2101 tgcataagga gccagttcgg gtttggtcgc cgctgcggac cgaaacaggg gaatggcccc
     2161 ggcatactgc ttgcggcgca tctcgatcag tcccaactgg tagtcggcgc gccccgggtc
     2221 caggggacgg gccttccgga gcatttccgc ggcacgagta tcctcattgc gttgcaggta
     2281 gatctccgcc aggtcgaaat aggctttttg aaaggagcgt tcgtcctctc gaagaagggc
     2341 cagaagaatg ccttcggctt cgtccgggcg attcaaatga atgtacacgg aggccagggt
     2401 gtattggtaa tcgagattga tgggatcctg gaggatggcc gtctggacct ggctgagggc
     2461 ttcttcatag ttccgtgccc gcagggcctg aaccgccaag tcgtgagaga ttgaaacctc
     2521 gggactcggg ggcggcaaga cctgggcaag acaaggcgca agccccacaa gcacgctcat
     2581 gaacgccagc cagtagcgaa gacagtgact gcctatcatc attggtgatc tcctgaaagg
     2641 gggcatcaac cgcccaaggg cactccacaa caccgatctc cctcaaccct tttttttacg
     2701 ggaaaatgct caaggacaaa cacaaaaaga ttcgatgaaa gcctgcgcga cgaagcttga
     2761 cggaatcgcg acggaccgcg atctcatgtt ccccgaggca tattccttca cgagaaaatc
     2821 aaagtcaacg aaaaagcttg gtaactcctg tttaattcca tatttactcg atacttgttc
     2881 agtactcgca cctccaaccc aatcccgcgg agcaaccgat ttctcccatg gtaaccttac
     2941 gaattaccgg caaaaatgaa gaacctattc ccgtgaggcc tttctcacgc cctcggggtc
     3001 gtcgggacgg atcttttgcg gggtttgccc cagaaaaccc tcctgtgccg ggaaagtgct
     3061 acggaatccg ggaagggctt tttcgaaggg atcaacgagg gagtgcggga catcccgccg
     3121 ccgccccccc ccggccgcgg attcaccccg gccctcccgg aacgggagtg gtcggcaagg
     3181 gaatttcaag ggattcatcc gcatgggggg tgaaccggca gacgagcact cagaggacgg
     3241 caatatcagc gatttcgaca gcatccgccc gtaatgcccg cgaaggcggg catccggagg
     3301 atttgcacgc tctttatgga ttcccgcctg cacgggaatg acgaaacggg attcgtaccg
     3361 gccaaatcgc tcaactcggg ggggagtctg tcaacagctt ttggaccgcc tggggaggga
     3421 gtccccggac ccgaagggtc ttcttgcggg acgtgcggcc tcgaaggatg tccacggaag
     3481 acctgggcac tcccaataat ccggccacat aacggataca ggcgtcgttg gcttcccctt
     3541 ccaccggggg tgccgtcaac cgaatcttca atgccccctg ttgcagaccg gccaactcgt
     3601 ttcgggccgc cctgggttgc accaggacct ggaaggcaat cccttccccg gtttccatca
     3661 ccaccagctt ttcccttggg acctcgtcct ttgcttccat gtccttgtga tttccttcca
     3721 tgcggaatcg agacccccgt tctgtcggaa ttgatagatt tacaggagcc ccgagtatgg
     3781 tttagagact tcggagggac cgtaacgagg atcgattttc ccgacacttt ccccgcgtaa
     3841 cgaggattca cgaccgaccc acacgaccgg ttcgaaatac gagggtaacc gattcttctc
     3901 actcaacaca aggagaacgt ccatggaaaa agtcctgcga atcgacatgg gagcggacgg
     3961 cggtccggca ttccgggagg agcccttggg agactacgcc gggttgggag ggcggggcct
     4021 cacttccgcc atcgtatgca aggaagtgga tcccctctgt catcctttga gctcccagaa
     4081 caagctggtc attgccccgg gactgctcgg cggcacgacg gcggccaatt cgggccggat
     4141 ttccgtgggg gccaagagcc ccctgacggg cgggatcaag gaagccaacg ccgggggcca
     4201 ggccgcccag gccctggggc gcctggggta tgccgccgtg gtgattgagg gcaagcccca
     4261 aacggacgca atccacaaga tcttcatcgg caaggacggc gtgaggatct ccgaagaacc
     4321 ccaactgaaa atgctcccca actacgacct ggtggaagtt cttcgccggg aacacgggga
     4381 cgggatctcc tgcatttcca tcggcccggc cggagaaatg aagctctgcg cggcctccat
     4441 cgcctgtacc gacatggaac tgcgacccac gcgtcatgcg ggacggggag gcttgggcgc
     4501 ggttatgggt tccaagggcg tgaaggtcct cgttttggac gattccggca ctaagccccg
     4561 ggcgccccgg gaccccgaag ggttcaaggc ggccaacaag gccttcgtgg aagggcttcg
     4621 ggcgcacccc gtgacgggac aggcccttcc cgcgtacggc accaatgttt tgaccaacat
     4681 tctgaacgaa gccgggggct atccgacctg taattttagc cgaggccgat ttgagtcggc
     4741 tcaccggatc agcggggaaa ccatggccga gaccgagacc gcccggggcg gaatgaccgc
     4801 ccacggctgt catcgaggct gcgtcattcg gtgttccggg gtctaccacg acaaagacgg
     4861 tcgttttctg tccaagcagc ccgaatacga aacggtatgg gcccacggcg gccactgcgg
     4921 gatctgcgac ctggacgcta ttgccatgct ggacttcttg gacgacaatt ttggattgga
     4981 taccatcgag atgggcgtga ccatcggagt tgccatgcag gcggggctga tcccgttcgg
     5041 ggatgcccaa ggggccgttc gcctggttga ggaagtcggc cgggggactc ccttggggcg
     5101 aatcctgggg agcggcgccg cggtcacggg taaggtgttc ggggtggaaa aggtcccggt
     5161 agttaagggg caggccctcc cggcctacga tccccgggcc gtaaagggag tcggggtcac
     5221 ctacgccacc agtcccatgg gagccgatca cacggcaggc tacgcggtga ccgccaacat
     5281 cctcaaggtg ggaggggacg tgaaccccct ccgttcccag gggcaggtgg agctctcccg
     5341 ggacctccag atcgccaccg cagccataga ctccacggga atgtgtcttt tcatcgcctt
     5401 tgccgtgctg gaccggccgg aaaccttcca ggctctcctg gacatgattc ggaccctcca
     5461 tggaatcccc ttgaccgccg aggatgtcgt cgagatggga aagaagatct tgaagatgga
     5521 gagagatttc aacctgcggg cgggtttttc ttcggcacac gaccgcctgc cccggttctt
     5581 ccgggatgag cccatcgaac cccacggggt gaggttcgat gtcccggagg aggaactcga
     5641 ccaggtattc aattggtgaa ccgagggccg gagctcttcc gggagcggcg gcgtccgtcg
     5701 agaatccgcc gctcccttct ctccatcgcc ttctttttct caaaatcgag gggtcggggg
     5761 ggaaacgtca cgggttggac gctcccatca accggttgtc cggaattcat ccgcacctgt
     5821 ccggccttcc tcagcattct gcccgccacc cgggtcatgg aagctggagc aggcaggccg
     5881 cagcggatcg ccgaactcgg gaggaacggt tgcagccgat catcgaggcc agggacctgc
     5941 gcaaaacctt cgcagacttc gtcgccgtgg acggcatcca cttccggatg atgccgggag
     6001 aatgcctggg gctcctcggc cccaacggag cgggtaagac cacgaccatt cgaatgatct
     6061 acgggttttc tcccattacc cggggcagtc tccgggtgtt cgggctggac atccgcaaag
     6121 aatggaggac catcaagggg cggatgggcg tatgccagca ggagaacaac cccgatcccg
     6181 acctgtccct catggagaac ctcgaggtct tcgccctcta cttcgacatc cctcgaaagg
     6241 aagcccggag gagggcccgg gacctgctcc gcttcatggg gctcgaacac cgcatggacg
     6301 ccaaggtgac cgagctctcg ggagggatga tgcgacgtct catcctggct cgatcgctct
     6361 tgaacaaccc ggacctgctg atcctggacg agcccaccac gggcctggac ccccagtccc
     6421 gccaccaggt gtgggaacga ctcgaggagc ttcgatcccg aggcctgtcc gtcctgctca
     6481 ccacccatta catggatgag gcagcccgcc tgtgcgaccg gctgatcctc atggatcacg
     6541 ggaagatcct catcgagggc aaaccgtccc ggctcatcgc ggatcatgtg gggcgagacg
     6601 tcatcgagat ggtgaatccc gacccggagg ttcgccggtg ggtgcaatcc agcggcttcc
     6661 ggcacgatga cctggaacgg cgcctcatcg tgtatggaaa ggaaggggag aacctcttcg
     6721 accgggttgg aaagagatac tgcagcgagg agtgcgtctt tcggatggcc accctcgagg
     6781 atgtgttcct gcggttgacg ggaagggagc ttcgagaatg agggggattg gcccggggac
     6841 cgtgtccagg aggtttttcc gagtctggca gcgaaacttc ctggtctatc gtaggatctg
     6901 gaaaatcaac ttcctgcctc cactcctgga acccttcttc tacctgctcg ccttcggagt
     6961 gggattgagc ggcctggtgg gaaacgtctc ctacggggga aaagagctct cctacgccgc
     7021 cttcatcacc cccgcccttc tggccatcaa catgatgcaa aacgctttct tcgaaaacac
     7081 ctacgcatcc tttgtccgga tgtactacca gaagaccttc gatgccatga tggccactcc
     7141 tttgtccctc caggaggtca tcctggcgga aatcatctgg ggggccacca agtccttctt
     7201 cgccacgggg gtcatgctcg gggtggtgag cctgttcggg ctggtgcact accctcacgg
     7261 gttgctgatc cttcccctgt ccttcctggc tgggatcgcc ttcgggtcca tgggaatgct
     7321 gttcacggga atcgtgcctt ccatcgatct cttcaacctg cccgtgtttc ttttcatcac
     7381 ccccatgttt cttttcagcg gcaccttctt ccccctggat gccctgccct cttgggccca
     7441 atacgtcgcc atggtgctgc ccctcaccca cctggtggaa gcgatccgga ggctgtgcgt
     7501 gggggagctc ccgtggaacc tcctgtgggg attgggttac ctggtcctgc tttgcgccct
     7561 cttcctgccc ctgagcaccg ccgttatgca ccgccgactg attcactaag atccaagatg
     7621 agcgatcttt ttccctgctt ggcttcaagg ggccggcatc aaggccgccc ccgtgcggta
     7681 acacgcgaac caatcctgat acccggtttt tgcgtgagta ggcacattcg atgagctgcc
     7741 ttccgatggc acgcaccctg gagaggaaca gtgcagcatc gatgcggctg aacccggcaa
     7801 ggtccgcctc aaatccattc acgtcaaagg ccctttgaaa acggccgtct cacgatatac
     7861 gtccttgcgg tgccctactt tcacaacata cacaacgaga tcttgatctt cgatggagta
     7921 aacgattctg tcggatcctt gtcggacgcg atactcatcc tgagcggaga gtttttcaca
     7981 gcccggcgga cgcgggttgt ccgcgagtct gccgatgaga gcaacaactc gttggcggtc
     8041 ccccttcgag tcgatggact ccgtttcctt cacggccgat gtggctttaa gaagaggctg
     8101 tatcttgaca cgccgtgtca gatctttgag gacgtcttcg gatctcgagt tcggttcggc
     8161 tttccgttcc tcagaggaga acaagtcctg agcatcttcc gcaagtctta gcttgacggc
     8221 ttcgttcacg agatccgaaa tggtttgatc cgtctctgca gccttgagcc gaagagcccg
     8281 atggaaatcg gaatccggat agaccgtggc tctcttccgg gatgccttcg tgtctgcacc
     8341 ttaacgtcta agcgtctgaa tgtgaacgct gatccgtcga catgctgatc cctcagccaa
     8401 ccaagcccca ccccttcttc ccggtccact tcccgatgtt ccgcggcaag cctctcccca
     8461 atcccgacct gccgttccat aagcaatgta tcaatgcgta gacgggcaat ctgaggcccg
     8521 atcaacatag cagtgcttca cggggttgta gtacatctag ttcccatgct gctcgtaatc
     8581 gagcccatct cgaacctggt gttcccaata gctgccttgc cggattccat gctcgcctat
     8641 tgactctcgg ctcatggtcc ttctccgggt ggaatacctc tggagaatcc ggcccttatg
     8701 agagcccagt gcatgggata gctgcaatcc tcctgcggca acgtcaaaat tgcacggagg
     8761 tggtcgggaa ggaccacgac aggatcgaaa tagaaaggac cccgctccat cacttcccgc
     8821 atcacctctc gaagcaaatt cacgggatcg atcagaaccg tgcggtcccg ctctgcgagg
     8881 tctaccgcga aaaatgggtt cctccaggca ggccggcacg gcggtatcgc atagattgtc
     8941 agccccgtcg caggttgggg ttcaattcag gtcggtgaaa tccacacccc gaaaaaccga
     9001 ttgcttctcc gtgggggagc gaattccatt cccataaagg gtagattttc gctttatctt
     9061 gacagggtag cgggtggttt tggcccggat gggtccgtcc aggcagcacg tgtccgaaag
     9121 cccgtgccca cccgtttgaa attggaaaat gaaaggggcg gtgatcgacc gcccttttca
     9181 accatggggg atggttgcag tgggcaatgc ggttgcatcc aagtccccat tccctcatgg
     9241 ggaagattgg ggtgaggccc catcggcctc gaaagccccc gtctctctcc cccgaagcgg
     9301 gggcgatgga ggtaggtttc gtcggtctct acaccgacaa catggagggc gcccggaggc
     9361 cgggtctggg cttttccggc ttccgggagc tctccgatct tagaggatgt accccgtttc
     9421 tccgtgctgg ctgagatcca gcccctgggc ttcgtcttct tcgctggccc tcaggcccat
     9481 gacggcatcc aggactttga ggatgatcca cgtcacggca aaggaataga tccaggccgt
     9541 caggatgctc gccgcctgga tcgccggctg gatggcgttc ccatagaaca ggccgtcgct
     9601 ccccgccgga ttgatgagct tgctggcgaa aagtcccgtc gccagggccc cccaagttcc
     9661 ccccactccg tgcactccca ccacgtccag ggaatcgtcg tagcccagcc tggctttcat
     9721 gttgacggcg atgtagcaca gccctcccgc caccagcccg ataaagatgg aagacatggg
     9781 ccccacgaat cccgagccgg gagtgatggc caccagcccg gccacggcgc cggagaccac
     9841 ccctagggcc gtgggttttc cccggtggct ccattccacg aacatccacg agagggcggc
     9901 ggcggccgtg gcgatgtgag tcaccacgaa ggcggaggtg gccagtgccc ccgaggtgag
     9961 agcactcccc ccgttgaacc cgaaccagcc gaaccagaga agggccgccc ccaggatggt
    10021 caaggtcagg ttgtgggggg ccatgagatc gaccccgtag ccttttcttt tgcccatgac
    10081 cacggcacag gcgagggccg aaactcccga gctgatgtgc accacgagac caccggcgaa
    10141 atccagggcg cccagctcgc ggatccagcc ccccgtcccc cagacccagt gggccagggg
    10201 gtcgtacacg aagattgccc agaggacgat gaacgccagg tagctcttga atttcatcct
    10261 ttcggcaaaa gccccggata tgagagccgg agtgatgacg gcgaacatca tctgaaagat
    10321 catgaatgcc tggtggggta cggtggcggc atagtcggcg ttgggctcga ggcccacacc
    10381 ccgcagcccg acccagctca ggtttccgat gacatggccc acgtcgggtc cgaaggacag
    10441 actgtaccca aaaagcaccc actggatgga aatgactcca aaggcgatga agctgtgcat
    10501 gatggttgcc agcacgtttt tccggcgaac catccccccg tagaacaagg ccaaacccgg
    10561 ggtcatgatc atgacgaggg ccgtggaaac gagcagccat gccgtgtctc cggcattcac
    10621 cgcaggaggc gcttcctcgg caaagaccgc acttgcactc atcaccagcc aggccaacgt
    10681 ccagcccacg acccggctgc ccggccaacc ccccgggatg acggttttcc gcattttctt
    10741 atcaagaaaa agacctgatc gattcattgg aacctcccgc gcgagcacat ggaatttgga
    10801 ggatgacgga gcatcccgta ttttggtaaa gactcagagg gcttcgggac ccgtttcctt
    10861 ggtgcggatc cgcatgacct cgctgatgcg ggaaaggaag atcttcccgt ctccgatctt
    10921 ccccgtgtag gccgtcttct ggatggcatc caccacctgc tcggccaact catccggcac
    10981 caccaccatg aggaggatct tgggcacgaa attcacctga tattccgacc cgcgatagat
    11041 ttcgacatgg cctttctgtc gcccgaagcc tttgacttcg gtcaccgtca ttccctggat
    11101 gcccaaggcc aggagggcgt cctggatttc gttgagccgg aagggcttca cgattgcttc
    11161 gatcttgatc atggttccac cttctctttc tagggggtgt acgtacccgg aatttttccc
    11221 gttccccggc aaaggcccgc ccaaccggtg cgggaatgat ctcgaggaac ccttctacca
    11281 agaggcgtgc caggggcgga ggaaaacggg aggtgtccca tgtaaccaat cgaattaata
    11341 cggataatta tggaagccgg aggatgggaa gcgcgatgaa aactcaaaag actcaaggtt
    11401 ttcgaaacga ttttgtacct acccgagagc cgggagtcct gtcgttacaa tttggtgccg
    11461 aaacggtgaa aaacttgaga gagacatggg cgggaacggg gactctcgcc tcggcgagtc
    11521 cctctaatga ggcggcccgg cagttccgag acccggtcaa tcctggagcg ttggccaaac
    11581 ggcaccttgg gattccccct tgaagagagg aaggggtgga accacaatcc ccccgctacc
    11641 ctccccctca aggccggaat tgagttgctc aaccatcaaa ccgcatccaa gacagcgccc
    11701 cctcgcggga ccaccgggaa gtctttccaa ggggggaagg gaaccctttc cctcccggaa
    11761 ccccgcagcc ctcggggttt acgcggccac gtttcgttcc ctggagctta cttgcgaaag
    11821 caggtaggcc tcgatgaatt cgtccaggtc cccgtccaga acactctcca cgtccccccg
    11881 ctcggtattg cttcgatggt ccttgaccag ccggtagggt tggagcacgt aggaacggat
    11941 ctggtttccc caggcgatat cccccaggtt gtcgtggatt tcctgcattt tgtcctgctg
    12001 tgcctgccgt tcccgttcat ggagccggga ccgaaggatc ttcatggcga tatcccggtt
    12061 tcggtgctgg gatttttcgt tctggcactg gaccacgatc cccgtgggca aatgggtgat
    12121 gcgcacggcc gagctggtct tattgacgtg ttgccccccc gccccgctgg cccggtaggt
    12181 atcgatccgc aaatcggagc tcttgatgtc gatgacgatc tgatcgtcca gttccgggta
    12241 gacgaacacc gccgcaaagg atgtatggcg ccgtccgctg gcgtcgtagg gggagatgcg
    12301 caccaggcgg tggatgccga tttccgcctt gaggtacccg taggcaaagc ggccggtgac
    12361 cgtaaaggtc gcgttcttga tccccgcctc ttccccctcg agcagatcca ccatttcggt
    12421 cttgtagttc tttcgttcgc accaccgaag atacatccgc agcagcatct cggcccaatc
    12481 ctgggcttcg gtgcccccgg caccggcgtt gatgctgacg atggcgttgt gttcatcgtt
    12541 ttcgtccccg agcatctggc gaagttcgag agcccggaga cttttgcgga ttccccgcag
    12601 tttctgctgc acttcctcca tggtcccttc gtcgttctct tccagggcca tggaaaggag
    12661 aaggtcgcat tcctcgaact cctggagcag tttctgccat tcctcgagga tttccagcag
    12721 ggaagaccgt tccttcaaca cctccttgcc ctgctcgggc cgctcccaga agtccggctt
    12781 caggagctgc ttttccagtt ccgcggaacg tttcttgcgg ccatcgatgt caaagatagc
    12841 ctcccaggtt ttggatgcgt tgggaaagct ccttcatacc ggccttgatt tctgcaatgt
    12901 cgatgttcat gggaccaacc tcctgcaatc taatcagtga ccccgtcgga gggttccgag
    12961 ggggatgggg atccgggaca aggcactctt gatcgtcccc gcccgggctc gatgagctgc
    13021 ggccagggca gaaaccccgg ttgcggagcc ccggcattct aggaggaagc gcggttccgg
    13081 cgcttccccg ggagcgtata cagtaagcac cctgcgcatg aagccacgca ggtccacgca
    13141 aaaaaatctc cccaggacgc gtacacggtg cgaatgttta ggggccttac ggggcaaacc
    13201 agccagcctt cttcgtccag gggaatctgt ccgcacgccg ctcccacggc gtcgaagacg
    13261 gtgctgattc ccgtattggc ggcacgaatc aggggccttc gaaactctac ggcccgccac
    13321 cgggcgattt ccaggtgttg atagggggcg ctggtgcgcc cgtaccaggc atcgttggtg
    13381 acgttcacca ggacggtggc tccgtgttcc acggcggacc gggcgagata cggaaagatc
    13441 ccttcatagc agatgaggac ccccagttgc tgctccccga aggcgatggg ctccggattc
    13501 cgcccggccg aaaagtctcc cgcagcctcc accaggcggt ggacgaagaa aaggagcctc
    13561 ttgaagggaa cgtactcccc aaaagggacc aggtgccgct tggcgtaggc tccctcgatt
    13621 cgaccctccc gactcaccag gtaggcccgg ttctgaaaac cgggttcccc tcccgtcctc
    13681 gtgacggcag ggcttccgaa gagcagcggt ttatcagttt ccgcgatgat ctcgttcacc
    13741 tggcccgtga gctgttcttc cactccgtag aaaaacggcg tggccgtctc gggccacacg
    13801 atgaggtcgg ggggcggatg gttctttgcc gccccccggg acaaccgggc ataccgggca
    13861 agggtctcct gttgaaacgc cgggtcccat ttcttgctct ggtcgatgtt cccttggatg
    13921 accgccgcat tccacgggcg aacccgagcc tgaagtccgt tgacatcccg gatgcgccag
    13981 gacccgtaga ccaggacaca acccaggaca aggcagaaga cggggatcgc ccacttgcgc
    14041 accgggggca ggcggaagca cccgaccacc gtggtgctgc atagaaccag cagccaactc
    14101 agcccataga ccccggttat gtccgccgcc tggatcaggg ttagaaacgg ggtttgggag
    14161 taccccaggt ttccccaggg aaatcccgaa agggcgtggg cccgaaccca ttccagagtg
    14221 acccagaccc ccggcagccc gaaaacccac agccggggag aatcttccca gaggtgggaa
    14281 cagagggcga agagggccgg gtacagggcc aggtagaggc acaggaggag cagaaccgat
    14341 gcggccacgg gaaaggggag cccgccgtaa tggtccacca cgtagcggat ccaatagagc
    14401 atcgtcagga aatgcaccag cccgcagagg aatcccagcc cgagggattc cctcgagctg
    14461 cggccgccca aggccgtcag taaagggacc agggcaaccc aggacaggta gggaagcgtc
    14521 gccttgggca accccagagt gaggagaatt ccgctcagga tactgagtaa aacggcaagc
    14581 cacatgatct tcgactcatg tttccggtga tttcagtgga aggacggtcg cggtggaaag
    14641 acggaggctg cttcaggaga agacctcgcc ggaggaccgg gttgccgtgg cttcaccctc
    14701 cagcttgcgg atgcggatct tttcgatttt cctgctgctg gccgcttcga tgcgcatctc
    14761 caggtccttg tagacaacga tctcgttcac ggccgggacc tttcccatga ggctgatgac
    14821 gaagcccccg acggattcaa acttcccttc gggcagctcc atatcgaagt aatcctccag
    14881 ctcttccaca tccaggcggg cattaacggt gatggaaccg tcttcatgct cggcaatgag
    14941 catctggtcc gcatcatatt cgtccatgat ttcgccgatg atctcttcga tgatgtcctc
    15001 gagggtcagg attcccgcgg tgcccccgta ttcgtcgatg acgatggcca tatgggactt
    15061 gtttcctcga aggtctttga gcacctcgct gatcttcttg gtttcgggga tgaagtaggg
    15121 gggccgtacg atcccggaga tgtccaggtc cacctctccc cagttgctga ggaggtcctt
    15181 ggcgtggagg gtcccgatga tgttgtcgat gttttccttg tagatgggga tcctggaatg
    15241 accggtgcgg aggatgagct ggatgatgtc ggaaatcccg gtttggaggg gggcgcacac
    15301 cgcgtcggtg cgggggacca taatctcgcg ggcgatggta tcccggaagg agaagattcc
    15361 ctggatcatt tctccttcgt cctcggatat caggccctgc tgttcccctt cgtcaatgag
    15421 ctgttggatc tccttttcga tattctgtgc atcctccacg cgagcgagtc ggcggaacac
    15481 cattctgagc caattttgaa accccctggc cgatattctt tccaatgctc aacccctctt
    15541 ttagagatct accgagcggt aacgccaacg gagggctcgg ggccgttgtg ttcaactgtt
    15601 acccagtatt atcgtagcct tgagatgtta ctaggcccgg ggcgggttgt ctagaaaaat
    15661 ataggagagg accttgtgga ccagtcgggc cgccaggaaa tcgggagcgg tcatcgaggg
    15721 aatgggaagg agctccatga cgtcaaatcc caccactctg tgctgttgta ccacccggcg
    15781 aaggcatgag agaacctcgt accagccgac tcctccgggc tcgggggtgc cgacggcggg
    15841 catgatggaa ggatcgaaca catccaggtc gatgctgata tagacatgcg gacccagctt
    15901 cgaggggatt tcttcccaga gggaggggtc ccgccagagg tcccgggcaa agaagcaggg
    15961 gattttccga tcccggaccc actgaagctc ttcccccgtc acggcccgga ttcccacctg
    16021 caccagggag caaagtctcg agaccgggcg catgacacag gcgttgctga aggcactgcc
    16081 ctggtattct tcccggaggt ccgtatgggc gtccagatgc agcaccgtga ggtccgggaa
    16141 atggcccacc gccgcctcga tcatccccaa ggaaatcagg tgttcccctc ccagcagaac
    16201 cggcagcttc cctgcactca ggagaaaatc acccacccgt tggacctggt tcaccatgtc
    16261 tcggggagag gaaaccacca cggggagctc ctccagggtg gcgattcctt cccggtggac
    16321 ttccgtcagg gtctcatcgt cgaaaaactc gagctctctc gaagcattca ggatggcccg
    16381 gggcccctcc cgggtgccgg cccggaaggt ggtggttccg tcgtaaggaa cgggcacgac
    16441 gacggcgttg gcggaatcaa accgagattc ctcggcattc aaccccaaaa acgaagaatg
    16501 gggtccctgg cactccggcg gccgggaccc caccattggc tcactcataa agaccttttc
    16561 attttgaacg ttgcatcaac atccgatcca tgcggaccac ctgcgcggat gcccggatgg
    16621 aattcggaaa ctcctgtcct cgatccaaca ccttgatttc acacttctgc attccgaaat
    16681 acctcttcac cgtctccacc gccagatccg tatcgaaggg cttgcaggaa aacatgtcga
    16741 ggctcaggta ctgtttttcc ggaaacgtgt gaatgctgat atgactctct gcaatgagca
    16801 caaatccgga gattccccag tcttccggcg tggatccggt gtatcggaac acgtaagggg
    16861 gcatgatctt ggtcatgtcc atccgggccg ggtattcatc cagaaagccg tagatcccgt
    16921 tgatgtcttc caacccggca cgatcacacc cataaccatc gaccatcaga tgaaccccaa
    16981 atccgaattc gggtttgttg cccatggcag tcacctccct tctttcagtg gagaaacctc
    17041 aggttctcct cacgagtcac tccattgttc accgccctcc gcctggagat gccaccgcag
    17101 aaaaaagcgc tgcagggcct caaacgcctt caactgctca cgcctcgatg gactttgcat
    17161 gaaacactac gggcggtact tcctgaaaca aagtaccaga gatgggatca ggatcagaaa
    17221 accgtcgccc ctagcgaaac atggatctgg ttttcagcca aatcacgctg cgacggcatg
    17281 gggtcgccgc cgccgctttt cctggttttc aaagaattgt ccaaagctta ccctgtgtcg
    17341 gccgcacgcc caccaaggtg tgccttatat aaaggaccgc ctatggagtg tcaagagaaa
    17401 atgatacccg gaaaagattc aaaaaaactc gaaaaaatga aaaaggcggg atatttccct
    17461 gaggatcccc accaggaaaa ttattccgac gactaaaaaa gcgaatcctt atccaaacaa
    17521 gccgtatttc ctcccaaatc gtgcttggcc cgatagaggg ccccatcggc ccgatgaatc
    17581 atgtcctcca catccagatc cacgtcccct gacttggccc gaaaccgggc catcccgatg
    17641 gacaaatggg tcggttccag ccccagggag ttatagttct gtcgaatgcg ttccgccacc
    17701 atcccggcct gatcccggtc cagatgggga aggagtaccg tgaactcatc gccgccaaat
    17761 cgaaaggccg tatccacgtc ctggcgaacc gaagccttga gcgccttcga aagctggatc
    17821 aagagctgat cccccgcttg atgcccgtgc ttgtcgttat actccttgaa gcgatccaca
    17881 tcgagataga acagaaacag ggcatgctcg tatcgaatgg atcgaaccgc ttccctccgg
    17941 acaattcttg cgaaaaaatg gcgattatac aggccggtga ggggatcatg aatggccaat
    18001 ctctccagtt cggtgcgcag ctcccgttcc cggatcaccc gctttacctt ggcttccagc
    18061 tcgtccaggc tgaagggttt gctgataaaa tccgcagccc ccgccgccac cacgtccgtg
    18121 taccggtatt tcatggcatg accggtgatg gcgattatgt ccgtaccgtt gcggttttga
    18181 accaggtagc gaatgagttc catcccgtcc atgcgaggca tatcgaggtc ggtgatgacg
    18241 atctggaagt cggcgtgctc cagcttttcc agggcgtcct gcccgtccgc ggccgtgacg
    18301 acctggtggc ccatataggt cagggcctcc gcaagcagtt cacaaacctg ttcttcatcg
    18361 tccacaacca ggattgactg gccgggcacc ttgtagtttt ccgaattcgg ttccataccc
    18421 actcttaacc cgctcttttc cttgagatgt cctcccccct ctccaacctt aatgcagtca
    18481 tggggttttt acaaatattt tcattgcata ccggagatct aaaggcctat catctttcga
    18541 ttattttggg agggaggaac cattatgcag tgtgttgcca ttgtctacaa gcggtcgcga
    18601 cccgaagccg caaagttggc ccggatcatc aaggattggc tgacaagccg ccatgtggag
    18661 gtcctttgcc aggaaaacgt cgaccagcag gggaggagtt gcgcatcgag cccgaagctc
    18721 gaaatccctc cccaggcgca gacggtcatt gtcctgggag gagacggcac gttgctcagc
    18781 gtggcgcggt tcctggccgg ccgcacaacc cccgtgatgg gtatcaatct gggtggcctc
    18841 gggttcctga cggaaatcaa catggaagac tgcttccggg aactcaaaga gatcctggga
    18901 aacaattacg aaatcgaaga gcggatgcgg ctcgaggtga ccatccagcg ggacggccag
    18961 gaagtcatcc gtcaatcggt tctaaacgat gccgtcatca acaagggtgc cctggcccgg
    19021 atcatcgaca tcaaaacgtc cattgacgga aagtacctca ccaactatcg ggccgacggg
    19081 ctcattgtag ccacgcccac cggctccacc gcctacaacc tgtccgcagg cggtcccatc
    19141 gtgtacccca cggcccgtgt ggtcgttttg acccccatct gtccctttac cctgacccat
    19201 cgtcccatca ttttgccctc ggaagtcgtc atccaggtgg aaatcgtcgg gccggcccgg
    19261 gacctgaccc tgacctgcga cggccaggtg ggctgtgaac tgtcccccct ggaccggatt
    19321 cgcatctcca cccaggccag ccccctgcga ctcatcaaaa ctccgtccat caactacttc
    19381 gagattctca ggaccaagct caaatggggt caggcggaac tgcgccccct cccctgaccc
    19441 gccatgtttc cgagctccct ctacagggcc ctcagccact ccggcctcag ggcacggccg
    19501 ccctcgacgg cctccgacac cattgccagg gcctttgcct tctccgaggg cattctcacc
    19561 ctcaggggaa ggtagttgga cgcatggttc gccaggaaaa gaccccgggt catgtgagtg
    19621 tgcaaaagca tttcccgcag ctccgccagc aactccctcg gttccggcac ccggaaaatt
    19681 ccccgggata cccgttgggc cgtttcggtg ttgggcagaa ccatgagggt cagggccccc
    19741 acgtagttcg gatccatggc gctcaatgcc tcccccgtcg ctttcgcatg gatccgggac
    19801 ctttccaggc ccgcaatccc cagaatgacc gtcaccgaaa gcttgatccc cgcccggcgc
    19861 acacgaacgc ccgcctccac catccgttcg gggctcactc ccttgcggat gtcctcgagg
    19921 acctgggcgt ccccgctctc gagccccagg tagacgatcc ccagacccag ttcccgcaag
    19981 gcattcagtt cttccggtgt cttccgaaga atgctcttgg cgttgccgta gaccccgacc
    20041 cggcgaatcc acggcatctt ctcccggatt cgctcgagga tgcccacaag ccgcggttgc
    20101 ggcacgatga gggcatcccc gtcgaggaga aacacccggt ggagaaacga gtagtttgca
    20161 gcggcatagt cgatgtcttg gtcaatgatt tcagggtctt tgattcgaaa acgcttttcc
    20221 ttgtaagccc cgcagaagct gcactggttg tgggaacagc cgaccgtgac ctggagcagg
    20281 atgctgtcgg cctcgctggg ggggcggatg atcattccct cgtaatgcat tcccgacata
    20341 ggctctgtgg cacctcctcg aacccggggc tccggcggcc ccggtcttct ctggtctttt
    20401 ccttcatgaa aggctacact ccgtttcggc tgccgggcga cggccccgcc ttaccgtcct
    20461 attggatcat aaacccggca tgaacggaat aggaggatga tgggagccat gcattccttg
    20521 tccagccccc taccccccga ggccaagtgc acccggtgcc ggtcccgggc caccattcgg
    20581 ctatccagtc accatgccct tttctgcccc gagtgtttcc tggttttctt tcatcgggcc
    20641 gtttcccggg ccatgaagga attcggggtc tccgccgcaa ctcccatcat gatcgccgtg
    20701 tccgggggaa aggactccct ggccctgtgg gacgtccttc atcagttggg atatccggtg
    20761 gagggtcttc acatcaacct gggcattcac gacttttccc gggcgtcttc cggggcggtg
    20821 gaccggtttg cccgctcccg gggcctctcg tggagggagg tcttcctcga agagatcttc
    20881 ggctacgcca ttcccgaaat tcgaaggaga acccgcagaa agatctgttc ggtctgcggg
    20941 ctcttgaagc gccagctctt gaatcgcctg gtcgtccgtg agggatatgc ggtgctggcc
    21001 acgggccaca acctggacga tgaagccggg cggctgttgg gaaacctggt tcgtcaccgc
    21061 cgccaatacc tggaaaaact ttccccgttt ctgccctcac cgcacccgag actccccatc
    21121 aagatgaagc ccctgtatcg cctggaagcc ggggagattc gtttgtactg tcaaattcgg
    21181 ggcatcgaac cggtggattc cctatgcccc ctttcgaggg gcgccaccag ccacgccttc
    21241 aaggaagccc ttcaattcct ggaggacaag atgggaggaa ccaagaggga tttcctgttc
    21301 ggctacctgg acaggcccgc aagggaagcc tcgatgcccg gttttgggac ctgcgctcga
    21361 tgcggagagc cgacgtacag cgatctatgc agcgtgtgcg gcctgaagag ccggttggac
    21421 ggcaagaaag gcaaagaaac ggacgccgtc ccgaacatcc cggcacccca gatcaaaggg
    21481 agttagggga aaggcccgac cctttcaaaa cctttccata gaggatcttg tggtctccgg
    21541 gggcgtaaaa atcccggatc agggcttcct ttccataccc gttgtgttcg tagaacgcgc
    21601 gggtgggctc gtagagcggc tgggacgagg tttccacgta aacccgggtg ccccctttgg
    21661 cctcaatcag ggcctcggtc cgtttcagaa gctctcttcc caggcccctt ccccgcactt
    21721 cgggccggac ggcaatccaa taaagatcga agctggatcg agtgcacgcg atgggcccaa
    21781 agcaggtgta gcccaagatc ccttccgttt cttcagcaaa aaggaaatag tacccgctct
    21841 tctcgccccg ctgcagggcc tcatccacca actcgacggc aacctccgtt tcccggtcgg
    21901 tgaaaaagcc cgaggattca acaacctccc ggacccactc ccggtcgccg gcccggactt
    21961 tctcccgaaa gttcacggaa gctcccctct ccaagcttac cgaatgttgc cggggttttt
    22021 cgtacgccga agacaccttc gcctcctcag tgcagttcca tgccggagga ttcttgccga
    22081 caaagagtcc ggcaggcttc cccggggccc gcacccattc gagcgtcttg tcccatcccg
    22141 tgctggtcga attcgaaccg acttacctgg tgggcgtttg gaccgagtcc cgtgagccgc
    22201 cgcaacacag ggcgttccgt ttgcagcgat tggtcgcaac cgccgttcga accggcgcgt
    22261 cttccggcct gacgcccggg agcccctttc ttgaatcctc catcgccggt gaagcccgca
    22321 ggcccgcctt tgaggcaacg gcaaaacaac gtgccccact cattcccccg gcactccgtc
    22381 cggcgccggg caagcctcca gaatcctccg gatcatttgg gggtaagaaa gacccgactc
    22441 ggccgcggcg gcgaaaaatc cagcatcggg cgacaggcag ggatttgcgt tgacctccag
    22501 gacccacgga ttgccgacgg ggtccactcg aaaatccacc cgagcatagc cgttcaggcc
    22561 gaacgcctcc cagcaccggg aagcaatgcg cttgagacgg caaatcaggg gagcgtcggc
    22621 ctccgcaaac tgaaaactcc tgggagtgtg ggcatactcg aaggactccg catcccactt
    22681 ggctcggtat cccaccagcc ttgtcctgtg gcgaggatag tcgtcgaagc ggatttccgc
    22741 ggcggggagg accttcattc ccgagggacc ggccagcatc gagaggttga actctctccc
    22801 ctcgatgtag gcttccgcaa agcagtcccc tcccaggatt cctcggcgtc gggacatctg
    22861 tcggcacaac tccggccaat cccccacctc caccaccgaa tcctcctcga tccccacgga
    22921 ggcatgttcc cagacggatt tgacgatgta ttgccccgga ctgaacggca accgtgcctc
    22981 ccccgggcga cccgaatacc atggaggggt tggaattttg gaggcctgca tccaggtctt
    23041 ggctatcggc ttgttggatg tcaggaagat cgcctcggcg gaggatcccg tgtacggaat
    23101 tcccaggtgg tccaacaggg aaggtgccag gtggatgagc cgccccgacc cgtccaggcc
    23161 ctccaccagg ttgaacagca tctcgggacg aagatcgccg agagaagcca gggatttccg
    23221 caagtccagg gagaaggaaa tggcgacggc ctcatgtccc agttcgctca gggccgacga
    23281 aaccgcctcc acctgaacca ggacgtcttc ctcatcgggg gtggccggtt gcgacaattc
    23341 attgtataga acggcgatgc gcatacggat ggttctttat gcagggattc ttccgggatc
    23401 tcgggattcc aggcggcatt ccttgcgggt cccggggcag ggggaattgg aggcccggtc
    23461 cagggcggat cggacgatct tctcgatcag tccagggtag gagatcccca cccggctgca
    23521 caggatggga agatccgaat gttccgggtg cagtccggca agagggttca cttcgaggaa
    23581 atgaggcttc ccacgggagt cggacctcaa gtccacccgg cctccgtccc gacagcccag
    23641 gcccctccac acctccaaag ccagggcgag ggctcgaaga gcctccggat ccgttgcaac
    23701 ccgatattct accagttctt cgcatctttc cttgttcacg taggagtaga cctcctgctc
    23761 cgcccggtcg ttgagatgca cctcgaggac ccccaccgcc tcggccgcct ttcccgtccc
    23821 cagaatcccc acggtgaact cgcggccggg gaggaacgtt tccaccagca ccggctgccg
    23881 gtaggtctcg atcagtcttc ggctgacgac acccagctct tcgggggacc ggacccggga
    23941 tgccggagtc acccccttcc ccgttccttc cgccaccggc ttcacaaaca agggataggg
    24001 cagcgcgatg gagcgggctt cctcgtggga ttcgagcacg gcgaaatcgg gggtgggaat
    24061 tcccatgtcc cggaccacgt gcttggcgag tcccttgtga agggtcaggg agaggaccag
    24121 gggatcggaa aaggtgtaag ggatctcgta ggcgtccaag attgcgggca cgagggcctc
    24181 acgaccgtac ccgtgcaggc cctcggcaag gttgaagacc aagtcccacc gatcccccct
    24241 tgccagccgt gccgtgaggt ccttcccatg accaatgcga tcggtgccga agccaaggtc
    24301 ccggagcacc ctttcgatgg catcgatggt gtccggatga tcgaactccg ccgtctcttc
    24361 ttccccgtag cccatggcca ggtaatcccg gcgcaggtcg taggtaagac cgatgaccct
    24421 cacggcccga ttccttcttt gcaaaggcag gcggggaact catccgggcc tttgtccggc
    24481 gaggtacttc cggcttcggc ctcttcggga taacgataga ggtccccccg gtaattgcgc
    24541 aggagcaggg acccgttttc ccgtcccacc gcgtattcgg gaagcagggg gatttttccg
    24601 cctcctcccg gggcgtcgat gacataggag ggcaccgcgt aaccggtggt atgccctcga
    24661 agaccttgaa cgatttccaa ccccctggac accggggtgc ggaaatgcag ggaccccggg
    24721 atgggatcgc attgatacag gtaatagggt cttacccgca ttctcaggag tccctgcatc
    24781 agccgtttca tggtatcgac ccggtcgttg atcccggcca gaagcaccgt ttgactcccc
    24841 aggggaatcc cggcgtccgc aagccggttg catgcgcagc tcacctcggg ggtgagttcg
    24901 tcggggtggg taaaatgaat gctcatccac agggggtgat agtgtcggag catccgcgta
    24961 agcaggccgt tgatgcgctg gggaagaacc acgggaacct tggtgccgat gcgcacaaat
    25021 tccacgtggt caatgctgcg caacgaggaa agcagccatt cgagccgttc gttggacagg
    25081 gtcagcggat cgcccccgga caaaagcaca tcccgaacgg cgggagtcgc ccggatatag
    25141 tccaacgccc tttcccactg ccggatggag cccatgtgga ggttcctttg gcgatctccc
    25201 accatcctcg aacgggtaca gtaccggcaa taagtggaac agaaccccgt aaccaggaac
    25261 aatacccggt cggggtaccg atgcacgatc ccgggcaccg ggctgtcccg gtcttcatcc
    25321 aggggatcct cggcctccga agggtgtctg agatgttccc tccggtccgg cacaacggcc
    25381 cgtcgaaggg aatgcgccgg gtcctcccgg tccagcaggg aggcataata aggagtgacg
    25441 gacagaggca gggaggtcga cgaccgaccg agggcctcga tttcgtccgc agagaggtcc
    25501 agcacccgtc ccagaaactc ggggtcctgg atccggtgtc tcaactgcca gtgccaatcg
    25561 ttccaatcgg ccagagtggc cttggggaaa tactgcttcc gaaaggcgtc ggatcgagca
    25621 ctcaccggaa atcgagatcc attgtgcaag accttgacct tgggagggtg cgattggttg
    25681 ggaggggggg ctacatctgg aatggactca agagggtcct ggatcggttg aacgggggga
    25741 acccgaaggg cctgacaggc cctactggga ggttcggaat cctcctccgg gggttccgtt
    25801 tctttgttga cgcagacatt ttcttccatc attgctctcc tgagttttac agcgaggtcc
    25861 acagatccaa caacaaacag gggtacccgc ggcttcgggg gaagagggcg gccgcacccc
    25921 gggacgcggc gacccctcct ccggacccga tcattccttg atttcctcca ccgatttcac
    25981 ctcgatggtt tctcccttac cgccttccgt aataacgccc gtggcttcca ccatctttcc
    26041 gaccattttg gacagatctt tgccctttac caggtagtcc ccgtcgtccg cttcgatgat
    26101 aattccccgt tccgtcttga tgaccattcc caccacggtt tcctcatcgg cggccatcgc
    26161 tcgattcacc caaacgaagg acacgagggc aatgaccacg gcacaaagga tcaatcccat
    26221 ccatcttctg tcccttttca tccgtccaaa ctcctgtgaa gtagtgtgat tccggtccac
    26281 ctctccgatt tcggcacttg atatcaaatt cgctcggcaa agtacaacaa taacttcaga
    26341 gcggcttcac ccaagactgc atcgcatgtt ggagcccgtc tcgggtgctt ctcaggattg
    26401 tgcccacccc acgggcttcc ttctttggaa cttgcccggg acccctcagg cgggagcgat
    26461 ttcggtcctg atcgccggtg gtttcgggtc tttgaaccgg ccgtctgcga tttttcgcta
    26521 aactccggaa tcatttcgct gtacagatag tcgggatcaa gacttccgcg cagcccacgg
    26581 gccacgctgc accgctgcga tccctcgcaa ggttgatggt ttcgtaaaaa gtcccgaaga
    26641 gtgcgtttct tcgaccctaa ccgattgaat tcattaggtt ccgttgcgag aagaaggact
    26701 tcttacggga tcatcaaggt cgggaaacgc accgccgggg aattcggttt cgggcagctc
    26761 agggggagac ggacgcacat gagaaaactg atcgtaagag tcgtcaagaa acttcgccgg
    26821 gtccttgcgc ctttggtcgt cccggcgttt catatcctct ggtatcacga ccgggccact
    26881 tggaagaaga acaccttcct gggataccgg attcgtcaat gcccgctgga tcttcatcta
    26941 taccaggagc tgatccatcg gacgagaccc cgctttgttc tccagacggg ggtcgatcac
    27001 ggcgggtcca tcctctactt cgccaccctt ttggacctga tcggggctcc gcccgaatcc
    27061 ctcgtggtgg gaatcgacat cgctttgtcc aaagacgccc taaagctcga ccatcccagg
    27121 attcgactgc tggaaggaag ttccgcggac ccggatatcg tgagccgggc aaagggcctt
    27181 ctgccccggg acggaggatt cgtggtactc gattccgatc accgaaagga acatgtgctc
    27241 tcggaaatgg agctctatgc ggacctcgtt tccgtgggtt cctacctggt ggtggaagac
    27301 acgaacctca acgctcatcc cgtctatccc gatttcggac ccgggcccta tgaagccgtt
    27361 cgggattttc tgcgccgccg ccgcgacttc gttcgggatg acgacgtttg gaagcgaaac
    27421 cggttttctt ttcaccaggg aggctggctg aaacgagtgc gtccccaccg ggccggttct
    27481 tagttcggtt ccccggtacc gggtatgcgc aacaggcttc gaatcggctg aggtcccgcc
    27541 gccgtccttg gtccccttgc ccctcccccc ccgcatgcag gaggacctcg agtgaacccg
    27601 tgccccgaca ccttccatcg agaaccccgg aaatccatga ctcaccccat ggcaaagccg
    27661 gttttcttgt cgtaggatgg cgtgaacggt cctctttcgt gaagaagaac acgctttccc
    27721 cctctcggga aagcctcgtt ccctcatctc ccaggccccc cgtccttttt tcggggacag
    27781 ggatccaagg aggcaggttt ggcgatggaa tgcgctcccg gttatggccg gatcaacgac
    27841 gacattcttc gtaccctgtt ccggcccggg aggttgtacc ttctctttct tgctctggct
    27901 tttttggggc gtcgtagtgg gggcttcctg ccgggcccac caaatttacg cgggcctggg
    27961 ggttgccgcg gtcaactacc cggtggtctg gggtacgcat ctcgtcaact tcgtatttta
    28021 ggtggggatt gctcattttg ggaccttgat ttccgccatt ttgtacctct ttcgagctcc
    28081 ccggctcacg gccatcgccc gaagcgccga agccatgaac gttttcgccg tcatgaccgc
    28141 ctggctcttt cccctgattc atcacggcag agtctgggtg gtctactgga tcctgccgta
    28201 ccccaatcag ggcaacctct ggccgaactt ccagtcgccc ctggtcttcg acgtccccgc
    28261 cgtctcgacc tatctcaccg tgagccttct tttccggtac acgggcatga tcccggacct
    28321 ggccgtggtg cgggaccgat ccacgggcgt ccggcgcaag agctacggct tctttatccc
    28381 gaactggagc aacacgtctc aacagtggag acaccacggg gccgcgtatc tttttttcgc
    28441 cgctctggcc actcccctgg tggtttccgt ccacagcgtc gtatcctggg actttgcgtt
    28501 ccccgtcgtt cccggacggc acagcaccat cttcgctccc tactccgttg ccggagccat
    28561 ccactcgggg cttgccatgg tgatcaccct tttcattccc ctcaggcgga tttatcgcgt
    28621 ggaacacttc atcaccgtgc acattctgga gcagcttgcc aagaccctcc ttccgaccgg
    28681 cctgatcgtg ggatatgcct acgtggtcga gttttttatg gcgtggtaca gcgggagcgc
    28741 cacggaatac gatctgttcc tctggcgcgc gttcggcaat taccgcgtca ccttttggat
    28801 catggtgctc tgcaacaccg tccttccctc ggtttttttc tttaagaagg ctcgtaccgg
    28861 cctcccgtgg ctttttcggt ctccctggcg gtgaacgtgg ggatgtggtt cgaacgttac
    28921 gtcgtcattt gttcctcccc cggacgggag cttgatccat actcatggga cgtcctccgg
    28981 gccgtctacg cccccacctg ggtggaatac ggcaatcccg gcgggctctt cttccatgtt
    29041 ctttttcctc tttctgctct tcgtcaagtt ccttccgtcc gtctccatga cggagatgga
    29101 ggagttgctg ccgccccccc cggcgggcag gggcgcagga atcgccgtga aaacgggtac
    29161 gtcgcccgga agaaatcggc tttttctcca atcttcccca ttgcaggcgc cgatcagggg
    29221 atggtatggt ctcctcgccc gcagcttcga aggacttcga gtggacattc acccaattca
    29281 gcaacttttg cgcagcaagg ggcgttttcg cgaaatcctc ggggtatttt tccggtacgg
    29341 tctggccgac tggctgacca cccttccggg aggactcctc ctggagtact tcccgggaat
    29401 ggacccgaac aagatccgcg aactctcgac ggcggaacga atccggatgg ccttgaccga
    29461 gctgggaacc acctatatca agctggggca gatcctgagc acccgccccg acttggtggg
    29521 acctgaaatc gcgggggaat tggcgaaact gcagtctacg actcccgcag accctcctca
    29581 agtcgtccgc gagatcctgg aagccgaact gggggcctcc ccggaggccc tctatgtccg
    29641 gttcgacccg gtggcgacgg cttcggcctc catcggccag atccaccggg cgcaactcca
    29701 cgacggcacc tccgtggtgg tgaaggtcca gcaccggggc atcgaggacc gaatccgcca
    29761 ggacgtcgag atggcccttc tgctggcgga aacggcggag cgctactcgc ctccgttgcg
    29821 cgggtaccat cccgttgcca ctacccggga attcacgcgc accctgctgg cggagctgga
    29881 cttcggccgg gaactccaga acctgctccg gtttcatcaa cggttccgcc atgaccgggg
    29941 aatccggatc cccacgccct atcccgccct ttcttcccgc agaatcctga ccatggaccg
    30001 cctggagggg gtggaccttg cccacgtgga agaccttcga ggagccgggt acgatctgga
    30061 cgaattcgcc cgccggggag cccgactgtt tttggacatg atttttcggg acggttttta
    30121 ccacgccgac ccgcacccgg gaaacctgct gatcttgccc gacggtcgcc tggggcttct
    30181 agactgcgga atggtggggc ggatcgatcc ccgtttgcgg gaacacatcg aggacatgct
    30241 catcgccatt gccgaagagg attccgccca agtgtgcgac ctggtagtgc gcctgggggc
    30301 cctcccggcc ggttttgacg ccgaagccct ccgaaacgac atcgaccttt tcatcgggga
    30361 atacggatac cagtctctgg aacagtttga tctcaccgga gccctgaacg acctcatttc
    30421 catcattcgc cgccacaaaa tcctgctgcc ctcgaggctg gcccttctct tcaaggtgtt
    30481 gatcatgctc gagggaaccg ctcgcaagct cagtcccagg ttcagcttgg ccgaactggt
    30541 gcagccgtac caggcggaca ttgtgcagcg tcgcctggcc cctcaacgca tgtttcgata
    30601 cgttcgacac ctttcccagg actggctccg cctggccgaa cagtccccca aggacctgtc
    30661 cggcatcctg gaaaagcttc gaaccggtcg ttttgacgtc agaatcgacc accggcggct
    30721 cgattccatc atgaatcggc tagtgaaagc aattcttgca gcggctctgt tcctgggttc
    30781 cacagagctc ctcagctccc ggattccgcc cctggccttc ggggtgtcgg tgccgggaat
    30841 ggtgggttgc ggattggccc tgtttctggg gatcagggta ctctgggcga tactcaggtc
    30901 ggggaaaacc acgtcggacg acgggttgta gggtactgaa gaaagccgag gccaataccc
    30961 cggcttccgt ctcacaggac caagatcttt tccgtttctt cagggaaaca aaccgagcgg
    31021 aacgcttcct ccaccgtgtt cgtcagctcg cttccgggcg gaaaaccctc catctcggag
    31081 agctttccct cgcatctttt cgcctcttct tcttcgtctc ccatcaactc ctgcccgttg
    31141 aacctcggga agtcgggagt cagccggtcc gtatagagat cccccccgtc gccgaggatt
    31201 tcggcgaagg atgaaatccc cagcggggtc atccaaaggg gtcttctttc catccgcagt
    31261 ctcgtaatgt cgatactgat gtgctcgttg ccggtttcca tgacgaaatc ctaccttttc
    31321 gggttttcaa gggcctggtt ttctgcggcc catccatact ctaaaaggta aaattgagca
    31381 cccgcggaat caatcgaaga gaggtcccga cccccggccg gacccgggag agtgcccgag
    31441 gaccggggtg aagaatcacg tgcgggggga ttcccccagc ccgtgcatgg agcggatcaa
    31501 ttcttcgatc cgttcctcgc tgaccaggaa tccgccatcc acgggatact cccggccccg
    31561 ggcgtggact gtcaggaacg gctcgttatc atccgcccag cctttgcggc cgtgttccac
    31621 gcgcaggagg tccgctctct tgagcttcca tacttgaaac ccacagtgga tgatccactc
    31681 cccgtcgatt tccaggtaat ggcgacggcc cttccacagt acggccgccg caatcaaggc
    31741 aaacaccccg cagaccagca gttcgaaccg gatggaaaga tcttcccggg caaaggccgc
    31801 cgcacccagg cagatccctc ccaaggcaag ggcaagcagg tacccgtggg gccaacgatg
    31861 agactgcttg taatacttca tgccgccttt cttccatcac gacgtgagag agtcaaacga
    31921 ggtcggcaca ggtccgggaa cggcaggcat gagtccgtaa ggcggcgggg gggaaggtcc
    31981 ccgaccgtcc tcggctgctc atgccggtcg gccgggcagg acgactaccg ggtaccgcta
    32041 ccctttccga cagtccgtgg gaccgcttga gaagacccga atctctcctc gccgagctgc
    32101 gcctcagcgg ttcagcatgg agagctgctg gagagagagc tgctcctcga gagtggaaag
    32161 atcgcccagt tcctgtccca gggctttggc ctttaggacc cgcctgagga tcttgccgga
    32221 ccgggtcttg ggcaaggagc tcacaaactg gattcgctcc ggcttggcga tgggccccat
    32281 ttcagacgca acatgggcct tcagctcatc ttcgaggcct tcggaaggct cgattccctg
    32341 ggaaagaatg acataacagt ggatagcgct gccctttagc tcgtggggca aaccgatggc
    32401 cgcggcttcg gcaacggccc ggtgagaaac cagggcgctc tcgatctccg ccgttcccag
    32461 ccggtagccg gaaaccttga tgacgtcgtc cacccggccg atgacccaga agtagccctg
    32521 ggcatccttc ctggccgaat cgcccgtaaa atagacctcg gactgttccc cgcgccaata
    32581 ctgctgaacg taccgctccg ggtcgttgag gagggtgcgc atcatggcgg gccatggctt
    32641 ccgaatgact agaaacccgt cctcgtccac gggtttggcg gcgtgacgat catccaccac
    32701 gtccgccacg actcccagta cggggtaggt tccggatccc ggcttgaggg gagttgtggg
    32761 gttcggagca atcatgaaca tcccggtttc cgtctgccac cacgtgtcca tgagggggca
    32821 ccgccctccc ccgatgacct tgtggaacca gcgccacgcc tcaggattga tgggctcccc
    32881 tacggaaccc agcaggcgca gggtcgaaag atcatagcga ctgggccagg cattgccaaa
    32941 gcgcatgagt ccccggatag ccgttggcgc ggtgtacagg atgttgattc catagcgttc
    33001 gacgcattgc caccatcggt tggggtaggg gtagttgggt gccccttcgt agatgaacga
    33061 ggtcgcccct tcgatgaatg gggcgtagac gatgtaagaa tgcccggtga tccagcccgg
    33121 gtccgccgca caccagtacc ggtcttcctc cttgaggtcg aaggtcatgt ggaaagtggc
    33181 cgagatgccc accatgtatc cgccatggac atggaccacg cctttaggtt tcccggtact
    33241 ccccgaggtg taaaggatga aagcgggagt ctcggagtcc aagggaacag tctcacactt
    33301 ggtggaagcg atggggagag tcaggaggtc gtggagccag taatcccgtc ccgcttgcat
    33361 gttcaccgcg tgacccgttc gcctcaaaac gatgcaggtc tggatggagg gactgtgatc
    33421 catggcctca tcggcaatgc gcttcaactc gacgatcttg ccgcgcatga agccgccgtc
    33481 cgccgtaagg agcaccttgg actgggcgtc tccgaccctc tcgcggacgg cctccacgga
    33541 aaagcccccg taaatcacgc tgtgaacggc gccgattttg gcgcaggcaa gcatggaaaa
    33601 gatcagctcg ggaactcggg gcatgtaaat ggacacaatg tcgccccggg ccacgcccat
    33661 gcccttgagc acattggcca gcttggagac ttcccggttg acggcaaggt aggaaaaggt
    33721 ccgatggctg ccgtcttctc cttcccagat cagggcctgt ttgttgcggc gccaggtctt
    33781 cagatgccgg tcaatagcat tgtgaacgat gttgcacctg gccccggtga accatcgata
    33841 gaacggtttg cccgaatcgt ccaacacctg gtcccagggc ctgtaccatt cgagacgtcg
    33901 agccatctcc tcccagtatt cctcaaagtt ctcgcaactc caccgataaa gctcttccca
    33961 ggtgctgaat cctttctgct tcatgtaccg actgaggttc gcgttctcta ccacgtccga
    34021 aggcgggtag tagatttcct cctccttgcc ccctccctct ctcaggatgc ttccggtcgg
    34081 attttccatg acttttctcc tccatgcgca cgcgatgcag ttccgatgct ccgatggtat
    34141 tggaacggtg aaccgtgatc aagcagcccc gtagttgcac gagcacctcg agggcgggag
    34201 ggcctttagg gtgcaggttt cagcctccgc ccccaagcat ttcgcttccg aaattcagcc
    34261 acctttcccg gcggaaagtc ggcattcccc atgagctctt tcgagacacg ggatcccaag
    34321 attggagtgt cccgggaagc gtcccgcccg tcaaagcgcg ggtcccggga ctccttgtgg
    34381 gattcagaca tgcctttccg cttccggtgc ctgcccaagc caggggggtc cggggcccgg
    34441 accggtcaag agtgccggac gcaccacgga cacgtctttt cgtgcagcga atctacgaac
    34501 gcaatgcgac gcatctccaa caccccctcg cccccccggg gatcgggtgg ggagaggaaa
    34561 ttgtcgcaca ggcttttccg gagggttcac gtcgatgaaa cagcttccgg ccaaaccccg
    34621 atctcggcgg tgtttcccgg aatacggcgg ggccgttccc cgcatttcgg tctgccgtct
    34681 atttcgtatc cttgaaggat gggtccgcat ccgtcatatc ttggtaaggg caggaaacta
    34741 ttagaaaagt cttgcgttgt caagagctgt gttctcctcc ccgacgtttt tcttcgtctc
    34801 ccggatcgcc caaccccggc agaaaaagcc gcacggggct ttcccggctc attgcacgtc
    34861 ggacagctcg agcacgagga gctgcttctt gatggggttg aagccggaat ctttttcgag
    34921 cagccccttg agtccctggc gataccactt ctgacccgag ctggtctgca gcttttgaga
    34981 ctcgaggccc gcaggaagac ggcctcgggc cattcgggac aatacctgga gccggacttc
    35041 attttccatg tccacctggg tcgccacgaa atccaccagg gcggcccatt cttccgcagt
    35101 gggtcctccc caccgccggg tgagggcgaa caaccggtag caaaccaggg ctgcgtcccg
    35161 atcttcctcc ttctgaaaac tgcggccgct gtaaacccgc atggcctctc ggcccacctg
    35221 gctgatgaaa gccaggtgga accagcgcaa ggtaaagaaa tatccctccc gagtcacttc
    35281 ccgtcccgcc cagtagaaag ggtttcccag gttcttgatg tggacggccc aacgggccag
    35341 tcgatagaga acgggattgc gtctcaccac tcgaaggatg gggtggtcgt tgatccgccg
    35401 atacacttgg tagtagcgct ggtagtcgct aatcctgcgg cttccgaaaa agcggaaagg
    35461 agcgatggcg gtcaggcggg agagacgaag gcccaccctg cgcatcaact ggagcaactc
    35521 caccagggaa gcctcgtatt cgggagtccc ggccgttcca tggtagactg ctgcaatgga
    35581 ttgaatgatg cgggtagcca aagcggtcag ggcttcgacg ctgagctgga gcctcccctc
    35641 aggccattcc cagacctctc tgcgcagact ccgaatgatc tccagcgctt cccggtcctt
    35701 ggggcccagg ggggtgagca gatccagctc ttggtcctcc cgggactcct ccaggatctt
    35761 tttccagcgc ctcctcaacc accacctggc gcacagcagc accccgacgg tcgtcagggt
    35821 caccgtcagg aacaggccgc tgatccagtg ttcccggagg aactgatcaa gaagctgtct
    35881 tcccaggcct tgttccatgt cagggacccg tgttttgacg gaaacggttc ttcctaccct
    35941 cgtctcgaag gaggaatctg cgaatgaatc ccgctccggg gacaagcagg ctcccgagcc
    36001 cgcgagccgg accccctccc ccgtacggtg aaccgaatcc ctcgtccggg acggccgtca
    36061 tcccggaaaa ggggcccgag tagagcttcc caaccgggca tgagaacgcg cgggcgtttt
    36121 cctgcaaaga cggcattcca cgcttcccat ggtttcgggc cctcccccga ataccgctcc
    36181 cctaccgacg cccggagcgg tccgtctctg tggagtcgtg cctaaaaggg cgcgtcctgc
    36241 cccgtcgaaa ccgtcgcccc atcttcgggt tctttcccgg tttcgatcca gtgctgcatg
    36301 agcttcagag ttccgctctc ccaggaagaa tcgagcctct ctcggaggaa tctctcgtag
    36361 agcaggttca gggccgaaaa tacctcttcg aggaaataca tcctttccag gaaccgcccg
    36421 tccgcgtctt cttcccgggc ggcggggggt tgtttgggag tcttcagccc cttgatgctc
    36481 cagagagtgc tgtccagggt gaagaggtac tcgttgtccc cggctcggag gaagagctga
    36541 atctgttcta cccgtttgcc ctggcgcagg gccgtgcggg cttcgtgcag agcattggcc
    36601 tgggtcgtgc agacaattcg ttcctttccg ccctggggca gcgacaggat cattctttct
    36661 cccaggtgaa tttccgcctt ccggtcctgg ggcaggccga gcaactcgtg gggctgctcg
    36721 atgaaaaacc acagccaggt caggaattca tagcccagaa acctcccttc ctccagggcc
    36781 cggatcccgc cggaagtcat ggaatgtaag gaatctttgc ggcgctcgtc cagctcaatg
    36841 aggtgggaag cccagttcac atgaaagagg ggaagcggat acaggcgaaa gtgtttttca
    36901 aagtgttcca tgaagatttc gagcatcttc gagctggtgg tgcccaggag catccaccgg
    36961 gccgaagggc tccacaccac atcgaagcct cccggcttgg gaaacgccct gctcaggagc
    37021 atggcctggg tggtctccag catttcctgc cgctggtgac gggaaggcca tttccccccg
    37081 ttttcgtccc gatgttttcg aatcgcttcc cgcatgtgtt gtttaagaac catggcgggg
    37141 acctttttct ggtccacgcg gaaatgaaag gcgacgtact cccccttatg gtgggtcgag
    37201 tactcgaagg aatcctcgaa aaaatcgtcc caggaggtga accccgcgga ccactcgagt
    37261 ccttcctcgc actcgcgaaa ccgaccggcg tttagcttct cctcgacata actccagagg
    37321 tcccccggaa gcgggtccgg cagaagataa cgaacaaaac ttgcggatga agattggatg
    37381 ggcatcgatg accgtcctgt atgtgaatgc tgcaatcagc gaatccatgg ggccaaaaca
    37441 acagagccgg cacaaaccgg ctccgaacct ggtgcacgtc ccgttgggtc cggccctgaa
    37501 acccggtccg ggcagggtcc cacccctcga agtgtccccg caggaacgac ggggcggaca
    37561 ccccttttct gtggggccgt accgaacttc ggctgtcttc ttcggtgatc ggcggacccg
    37621 gacgaaccct cgggcaacaa gctgctcaga actttaccgt cttgacaatc atttcgtatt
    37681 cctggatctc ctcctggctc agttcccggc gcagaagctt tcggtttacc aggatgggaa
    37741 tcttctcatg gagggccagg gccaccgcat cgctgggtcg gctgtcgaag atcaattctt
    37801 cgccctgcag ccggacgtac accttggcat aataggtatt ctcctgcatg gagaagatct
    37861 cgatatgatc gaaaaccacg cccgctttcc gaagaatgcc caggtaaaga tcgtgggtca
    37921 ggggcctctt gggttccacc aggccctttt ctttggcgat ggccgtgatc tcggcgtccc
    37981 ccacgaacat catgaagtat tcggcggaat cctcggactc tttgagaatg accatgttcc
    38041 cctggatgcg atcggttttg atgaacacct cgttgaccgc aatgaactct cttgcctcca
    38101 tggcttcctc ctggaccgtc agggttgatg tgtcttttta tatagtaatt taaagggggt
    38161 ttgcaagaag tcttcggacc ggggcccgga gacggcgggt ttcggcgccc ggggcccgga
    38221 aggaccgggt tgacaaaccg gggaggaatc tttatacacc ctatcaattt acaaggtgga
    38281 tccaggtgaa tttttgcggc tcaaccaact gacctatcaa ccagaggtgc aactcatgaa
    38341 agtctgcttc ccaacggaaa cgctgcaggg tctccaaagc acggtgtacg ggcatttcgg
    38401 gtcggccccg ggcttcgtca tcgtcgaaac cgattccatg actttcgagc agatcgccaa
    38461 cgccgacctg catcatgctc acggcatgtg ccaacccctg cgggcgctcc agggacgaaa
    38521 ggtggacggc gtcgtagtgg gcggaatcgg aatgggagcc ctcatgaagc tgaacgccca
    38581 gggggtcgcc gtctaccggg ccatgcaggg aaccgttcag gaaaacctgg acttgatcct
    38641 ggcgggaaag cttccttctt tcacgaccga ccatacctgt gcaggtcatg ggggcggcgg
    38701 aggatgcgcc cactagaatc catgggccgg aaacccccac cattggaaag gatcgtcaat
    38761 gcccatttac gaatatctgt gtctggactg cgggaccccg tgcgagcaac tggttctcgg
    38821 gttggatgag gacgtctcgt gcccttcgtg caagagcact cgacttcgga agctcctgtc
    38881 cgcttcctct ccggcatccg ggccgaaggg ggaaggacgg cttccctccc agacggatac
    38941 cgcgtgctgc ggttcccgcc ccggggcttc ggggtgcatt ccgggatcgt gctgcggcaa
    39001 atagaaggga ccattgggtc gtaggaaagg cccttagagg agcacccgcc agcgggtccc
    39061 tccgggggtg tcttccagct gaatcttttt ttgttcgagc tgggcccgga tccgatcccc
    39121 ttcctcgaag ttcttttcct gcctcgcctg ttgacgcctc cggatcagcc cttccacttc
    39181 ttcttcggtc attcccgtag accggatttt cagggagcgc tgctcccgga ggaagacttc
    39241 cgggtcccgt tggaggatgc cgaagatctg cgcatgctcc cgcagggcct ctcccgctct
    39301 ccgggcaagt cccgccgcgg gacctttgag cttcttgcgg ccgaaccggt ccagaaatcg
    39361 ctggagatgg cgctgaacgc cgaagaggtg cccgatggcc tgggccgtat tgaaatcgtt
    39421 gtccatggcc tcccggaaaa gctccggcag ggtcgccgtt gcttccagca gttcggcatc
    39481 ctgttttctc aggacttcat cggcaaccgt ttccccgggg ggatcgcttc tttccaggac
    39541 ggctcccagg gtaccgtaga gccgatccag accccgttca gcctccccca ccgattcatc
    39601 ggaaaaat
//
LOCUS       DAOOVC010000021        39079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_667_length_39079_cov_6.231985, whole genome shotgun sequence.
ACCESSION   DAOOVC010000021 DAOOVC010000000
VERSION     DAOOVC010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 39079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39079
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_39079_cov_6.231985"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="PLM79_04750"
     CDS             complement(<1..1105)
                     /locus_tag="PLM79_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV85648.1"
                     /translation="MTQSFIFEQGPIRPPSEAGSLLLRFTRNCPWNRCTFCPVYKRAE
                     FSRRSLEEIRRDIDAVAGILEDLHRASVSMGAGGRITREVLSLSLRRHAQNPCYHHVA
                     LWHASGRGTVFIQDANSLVLPTDLLVQALEYLKKRVPQTRRITSYARSSTLARKSVAE
                     LREIREAGLDRIHVGLESGSDRVLRAVKKGCTAEQHILAGRNVIEAGMTLSEYVMPGL
                     GGRELSREHALETARVLNAVDPHFIRLRSLRVPSRAPLYREMKAGRFLPPGDDDTVRE
                     IRWFLESLDGIHSVLTSDHIMNLLEEVAGKLPEDKEAMLAVIDRYLALPPEERLLFRL
                     GRRGGALRSLDELQDPATRQRLQSAKRELESETG"
     gene            1464..1910
                     /locus_tag="PLM79_04755"
     CDS             1464..1910
                     /locus_tag="PLM79_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOV85649.1"
                     /translation="MRLTRAGEYAVRCVLSLAGRKPGEVVSRKQIAAEMDIPDQFLGK
                     IAQQLARAGILEIVQGARGGFRLRVPPDRLSLLEVVEAVIGEIFLNDCLMRPESCLRS
                     RSCAVNQIWEKARDQLRATLGEFTFSRILETQCCLVPPGEPGENPP"
     gene            1924..3237
                     /locus_tag="PLM79_04760"
     CDS             1924..3237
                     /locus_tag="PLM79_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOV85650.1"
                     /translation="MDPLILSRLQFAAATMFHFLFVPLTLGLSILVAVMETRYARTGE
                     EIYLRMTKFWGKLFLINFALGIVTGITLEFQFGTNWSRYSAFVGDVFGSLLAIEATAA
                     FFLESTLIGVWIFGWKKLSAKAHAVVMWLIAGASTLSAVWILIANSWMQHPVGYVIRN
                     GRAELESFSAVVFQEWALLEFFHTVAGAYILSAFFVMGISAYHLLRNRHVDFFSRSFR
                     IALVFGLVFSIFEVIEGHGHGADLYEKQPTKLAAMESHWETSTRAPIHLIAFPDEKNE
                     GNAVALLPIPGALSMLANHSFDAEVKGLKDFPPAERPPVLITFVAFRVMVTLGVFFVL
                     VTLYAWFRRDRLPESPRLLKLLVLSIPLPYIANQAGWVLAEVGRQPWIVYGLMKTSDA
                     VSPIHGSQVLVSFIAFLVVYGILGFAGFYLMVKHAKQGPVEVPQG"
     gene            3299..4327
                     /gene="cydB"
                     /locus_tag="PLM79_04765"
     CDS             3299..4327
                     /gene="cydB"
                     /locus_tag="PLM79_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOV85651.1"
                     /translation="MWLQDVWFFLWGLLWAMYFITDGFDLGIGSLVPFLGKEEADRKV
                     MLHAMGPVWNGNEVWLLTAGGVTFAAFPKVYAVMFSSLYSALMLVLFALILRGVYFEF
                     RGKLDDPRWRKVWDGCLFLGSVLPAVLLGVAFGNIFKGIPFDENGVYHGSLFTLLNPY
                     GLLGGALFLLLFLVHGAVYLAVKSEGDLHRRAVATANRLWPVLLVAAVFFFAATQVHT
                     RLYDNYFAKPILFLVPLVAVAGLLGIKMFLRKQAVGKAWISSAVTIAGAVFFGVIGLY
                     PALFPSSMAAGAGLTAHNASSSPLTLSIMLVVALVFVPIVLAYQGWAHHLFRGKITSG
                     DPAYEEVH"
     gene            4799..6667
                     /locus_tag="PLM79_04770"
     CDS             4799..6667
                     /locus_tag="PLM79_04770"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOV85652.1"
                     /translation="MIQLSEVQKQAVEYMGKPTLVTAGAGSGKTRVLTAKFAYLIRIG
                     YDPARILAITFTNKAANEMKDRLERLTGLKSFQFPWVRTFHSACLRILKEHCLRAGYR
                     HPLYVCSTYHQEKHIKALLVENFNLDPKKYLHPVAAAISRAKNSGRPRQYLEGIRVDR
                     RIQKSLPDIYAEYNRRLKGANSVDFDDILLRTRDLLDQHEDLRRRYADHFQYVMVDEY
                     QDVNDLQEEITRLVLKDGNLFVVGDDYQAIYGFRGANVGNFLDFRQRHADAVIFKLEE
                     NHRSTREVVETAELLIRKNRQIPKKCFSRKTGGSVEVAALENEFAEADRVVDLIRHHH
                     RSGIPYQAMSILYRSNAISLPFEQELRANGVPYMLKGDKGFFERKEILDINCYLAAAA
                     NPRDNVSLDRILNVPKRGIGSSAYRKILDLKHTDMSLRDACREAVRRMVLPTKVLLGL
                     ERLLDFLESIGKEKPEKAIGRVLSEAGYETYLAEYCKGENTREFQNRMENIQFLLYAA
                     SQKQDLVEYLEEITLDGSDEKEEKDESKSGVTLSTVHGAKGLEWEVVFVVGLEEDLFP
                     HWRALAQDSADGIQEERRLAYVAFTRAIRHLHLSWVGVRKGQSRNPSVFLKEAGLL"
     gene            6988..8268
                     /locus_tag="PLM79_04775"
     CDS             6988..8268
                     /locus_tag="PLM79_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85653.1"
                     /translation="MLQGRCGSGWFRGLLLLSFLPAALLPSPGFCAAPAPRFEVPINQ
                     KASDILPPQFLSGPHFRVRERVVSYGYMHHFTVDSDFGVFETTGDFALRKLVREIGAI
                     AALHQVKRGEAYAQGLKKAASQPLEFGANLVTDPVDTISGIPKGINRLFENVKTGLTS
                     KSQPGEDGKVEQALAVSSNKRELARRLGVDVYSSNSVLQKELNSVAWATTLGSLTLTA
                     ALAPVGGPAVAAVSATRTAQQVSDIVNEYPPQRLRQMNEEKLRTMGVSPGLIAQFLDH
                     PVYTPTHDTVIVGCLDALSGAKGREAFLQLALAADGEETANFFQNMAETMRYYHLKVS
                     PLREISVYGPLAFSEAANGTTLISFPLDHGVWTERAAQRVPGVISAYKSAHPRSKNLE
                     VWVTGTLSPMAKKELNKLGVRVVEKVSGQIEFVY"
     gene            complement(8402..>8733)
                     /locus_tag="PLM79_04780"
     CDS             complement(8402..>8733)
                     /locus_tag="PLM79_04780"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOV85654.1"
                     /translation="GKNLGANPVEFVLTALSGCLTTSLIAQAAVRGIRLRKVESRLEG
                     DLDVRGFLGMSEEVRNGYRNIRVTFKIKGDASQEELRELLELARKRSPVFDIVSHPVP
                     VSVELEA"
     assembly_gap    8734..8833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8834..9197
                     /locus_tag="PLM79_04785"
     CDS             <8834..9197
                     /locus_tag="PLM79_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85655.1"
                     /translation="FHSRTSEHKSMDEKVTAIYVMARKDELKPEHGGSYDTLLKSQEE
                     EIREFLRLKTGGNLQGRVEVYTRRAQLLMDIERSRVRRLAVESVERLGSSREEIEGIL
                     FELKMEGVELLSVRDNPA"
     gene            9222..12740
                     /locus_tag="PLM79_04790"
     CDS             9222..12740
                     /locus_tag="PLM79_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOV85656.1"
                     /translation="MEELAKDQPSTVIQGGSHGLVADFEERRRVLDPGTSFHLEAPAG
                     SGKTTLLTARFLGLLATVGHPREILALTFTNKAAAEMRERISQVLYRAGEGKEGDNPF
                     EVALLDSARRALERHAQFRELLFRGQFLQIQTFHSFCFHLVTQAPLEAGISPGSVLMD
                     EEEQGFFLREVIDEVLRGLLRASPGDPLRDALEDRLLYWNNSWSCLARELAELMERRE
                     SLGELLGFLDLEKAADYVDRWIGQLVEGKLAVLRSGFARSSLGQGWSDFVSHLESRGA
                     PAAELLPVALPEPRRESLESWQKIADVLLTQRGGVRKSVGPASGFYSGFAKTSWFEAM
                     HSLDSDTVARLSEIRDLPAEESVRLDLEPLWDLLLLTGEVIKEYEDRCRARRVLDFSS
                     LETAALRLFRQIRPSDLELYLDQRIHHVLVDEFQDTSRQQWELLQGLCAEWTPDGGKT
                     LFLVGDPKQSIYGFRKAEVRLFSDARAGLPLAGTGQRLPVEPVILRTNFRSHPSLIHW
                     SNRLFSKTVMEGSDGQAGGVEFMSAVPAPEVRRTLSSYNRLALFVPYPDSVSARQREA
                     RWMADRIFGELSSGDSRPEIGILLFTRTHLPVYLEALQARDIAVRVAEGMLLKERPEA
                     LYLWQLCRALVLPQDHLAWASQLHSPWLTLGYREILEISKEEPTPWVEKIRVHGEKNP
                     RVGEFWERLLYARQHLGHEPLADVLERTWLDLGGPEAVGERWGSRGLASSFRLLELVR
                     RAEVHEPVQTLDRLEELLERAYEPIDPDAAQSTVSMMTVHRAKGLEFDSVYLPFCDWD
                     PLARERGDRPAYLLERAEGTDGRMLLAVHPDRRTGEVDPTYRLVHRLRLDRRWQEAKR
                     LFYVAVTRAGSRLSLSGVLSGGRREGPPSFPARTPLGWLNDHYALEENLDLASPDFSA
                     VGEGNGPAGSPAAAAGKREWESREGDFRVWVEPAPESAESGLPSGPPLPEIRPAEFFR
                     ERPVFRVRSPSSLVGAKGEGIEVAEASSDGLPVDPALWGTLIHRILETWGREATLPDA
                     ARVRSYLRLEGMDEKDADRWARESLEEVEACLEDPFLHRLYSLPSGQRWVEWALESPR
                     SERVLDVGVIDLVCRDGDAWVLVDFKTSRPSDSQDPNAFCRDGLMRYRPQVEAYRGLW
                     ARWKKIRPEDIQAGIYWTALRRWDPC"
     gene            12751..14163
                     /locus_tag="PLM79_04795"
     CDS             12751..14163
                     /locus_tag="PLM79_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOV85657.1"
                     /translation="MVNAKHAFLLGGTHSGCGKTTLALALMAAFEARDLRVQPFKVGP
                     DFIDTGLHGRVAARESRNLDGWMLSHSYNCELFQERMAGADLAVVEGVMGLYDGYDGL
                     TEAGSSAQMAKRLGLPVILVVDASSMARSAAALVYGYSKFDPDLNLAGVVFNRVGGKG
                     HLEYLKEAMASSLPRVTVFGGIPREEAVRIPERHLGLLTADEAQLTPQWRSKLVELVE
                     RHLDLDEILRKSVYSGPVTAGEKEKPPRSPRPVPIAVAKDAAFCFYYADNFDLLTRWG
                     ADLRFFSPLAGETLPPGSAGLILGGGYPELFASRLAGQGRFLAEVRRAAAAGMPVYAE
                     CGGLMTLSRSIETLEGEVFPMAGILPFRTRMLSRRKALGYTEVELRSSCLLGEPGQVI
                     RGHEFHYSEIADMDEGESLERVYEIRGRKGSEPRPEGFATGSVLASYIHLHWGSSPGA
                     PRHFTQRCRQFESSRRERRF"
     gene            14160..15734
                     /locus_tag="PLM79_04800"
     CDS             14160..15734
                     /locus_tag="PLM79_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOV85658.1"
                     /translation="MSSKAKSLMFLGTGSDVGKSIVTAAFCRILKQDGYRVAPFKAQN
                     MALNSFITPEGGEMGRAQVVQAEAAGIEPHVDMNPVLLKPTSQMGSQVIVRGKAVGNF
                     SAQEYYRYKKTLVPVVRECFQRLAREYDVVVLEGAGSAVELNLKEHDLVNLAMAEMAD
                     APCILVGDIDRGGIFASLLGSLALMTEAEQQRILGFLVNKFRGDPQLFRSGIELLEEK
                     SGKPVFGVIPYFDHISIPEEDSVALDRRMKGRGLPAGEGRLRIGVVRLPYISNYTDFD
                     CFEQDPDVQLEYLDRPGRVFRFDAVILPGSKNTLDDLAFLKRSGLADALVAFYKAGGT
                     VVGICGGYQMLGLRVRDPLGVESGLVEVAGLGLLEVETEMFAEKITSQVEAEMLFGFG
                     REAPSGVRAPVLYGYEIHMGRTSPVRGSGRPLFRITRCGGRPVSVLDGFVQPEGRVWG
                     TYLHGVFDNDEFRKRFLEDLGTRSGREDVMSSTSFSFMRWKEEQYDLLADHLRKHVDV
                     EKIYGLIGLEKSGRNH"
     gene            15739..16755
                     /gene="cbiB"
                     /locus_tag="PLM79_04805"
     CDS             15739..16755
                     /gene="cbiB"
                     /locus_tag="PLM79_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOV85659.1"
                     /translation="MIPLLFFPWHLMAGYGLDLILGDPRGWPHPVRWIGFLITRLERV
                     FYQNSPDAVLQRLAGFFFWTAVVLVTASATVVLLGTAYFIHKILGHLVMIWLVYSTLA
                     TRSLHKESSKVVEALGRGDLDLARARLAYIVSRDTSALGEKDILRAVLETVSENISDG
                     IVAPLFYLGLGGPVGGMAYKAVNTMDSMVGYLNDRYRHFGWFPARADDVLNWVPSRLS
                     GLLILGAAALLKLDWRSSWRILRRDARKMKSPNAGYPESAAAGALGIQLGGTNLYFGK
                     PVEKPTLGDPLRPLSLEVYRSMIRLLYVSSALAFLAALAAQLGLYGIPAALSPMSLLK
                     EWFL"
     gene            16752..17858
                     /gene="cobD"
                     /locus_tag="PLM79_04810"
     CDS             16752..17858
                     /gene="cobD"
                     /locus_tag="PLM79_04810"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HOV85660.1"
                     /translation="MTLAHGGNVYEVASRLGCSPEAILDYSASINPLGPPPGLWDHIR
                     PSFRRVEHYPDIHNRGLVQSLADFHTQDPRRVVVGNGSTELIYWLPRAFGLRKALMVL
                     PAFGEYQRALEIQDVELYKIFTRPEDNFQPTVEDLEGAMERFSPDALLLTNPGSPAGT
                     VLPEEVRQWALERSRKDSFLCVVDEVFVDFCEEESLKGHVEKLDRLVVIRSMTKFYGV
                     PGLRIGYLFASRKLAERLRGFVPPWSVNTVAQVAGEYCLNRPEYRLETLSLIEVERTR
                     MARALGELPGCRVFPGAANYLLLRLPETFAPARVLRDDLLASHRVLVRDCSSFEGMDD
                     RYVRLAVRLPEENRKVLQGIAQWWRGQGKVGGRG"
     gene            18194..19675
                     /locus_tag="PLM79_04815"
     CDS             18194..19675
                     /locus_tag="PLM79_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85661.1"
                     /translation="MKTLLKCAIVVVALTTALSTVYAQTGSLMMPDLCNFEEWAQQVK
                     NPTPWLTWGADFRVRDEYLPNTVTLTDKDPLAEQNVARFRARVWGTAKMPSDVSFNAR
                     LSAEPRLFTEPAGFGPRKTASGMEWRYGILDNVNLKWNNAFQQPLTITVGRQDIMLGD
                     FYDWWLVADGTPGDGSWTFFLDSVRLTYDAKNIDTKFDVITILQHARADEWIPTIGNP
                     TYSDWPRFNDTEHPLTEQNETGVIAYLSNKSLKNTAIDAYFIYKRDDQETMERGGFPW
                     IPGDNADIFTFGGKISGNITPNWQYSLEGAYQFGNKEDRILGVFADRDISAYGAKAKL
                     TYLFKDKLNNQLFVSGEFLSGDDPSTPGTDEMFDVLWGRWPRWSELYIYSYAGETSGK
                     IAQMNNLMRFGPCWTFNPTKGMTFSVAYNALFAQEEVPTRAVAPRFSNNGDFRGHFLQ
                     TVLKHQFNKHLSAHIWVEFVWQGDYYARRDVMGFVRPEILFTF"
     gene            19932..20486
                     /locus_tag="PLM79_04820"
     CDS             19932..20486
                     /locus_tag="PLM79_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOV85662.1"
                     /translation="MRHRLVPRREDSLLLVIDIQEAMLKVIDPWEEPVRRTNQLIEAA
                     GILGIPVLATEQYPKGLGPTVPEVAKNLGPSAIFPKEHFSACLEEGFLDRVREFSRSQ
                     IVVTGMETHVCVLQTGMDLLANGFQVQVVKNAVASRFREDYQTALDLFRQAGAVVTSA
                     EIVIFQWARRSNTDEFRKILPVVK"
     gene            complement(20645..21565)
                     /locus_tag="PLM79_04825"
     CDS             complement(20645..21565)
                     /locus_tag="PLM79_04825"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HOV85663.1"
                     /translation="MNKTRAFVLAFLLFSLGTSFAFAATVRYTYDELNRLTRVEYEDG
                     TIIEYGYDAAGNRTSYKTIPSSGSLTMALSPARAVDDGARWNVDAGVWQPSGATVSGL
                     SVGSHVVSFKTLAGWTTPAAQNLAVAANQTTSGTGNYVMQKGSLKVTLKPNGAVAAGA
                     RWKVDDGTWKESGATVSGLQAGRHAVYFKNIKGWTKPAKKTVSIQQDRTKAVTGTYVL
                     RTGSLLVTITPKGAVAAGAKWKVDTLPWKDSGDTVPNLPVGKHSVQFRKIGGWKAPAG
                     FTVTIKRDVTIKASRSYVKSPGSSEGIAEE"
     gene            22570..23007
                     /locus_tag="PLM79_04830"
     CDS             22570..23007
                     /locus_tag="PLM79_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOV85664.1"
                     /translation="MVSAWHLWVIVGILLFIAEIFTPGFVLASFGIGCLFSALAAGIG
                     FSLKMQILGFIVGTLVAYFTVRPIFTRYCYKSSPDVKTNVDALIGKIGRVTETISLEV
                     GTGRVLVGGDDWKAVSVENTIIPERSPVEVVRVQGTTVYVRPV"
     gene            23032..24042
                     /locus_tag="PLM79_04835"
     CDS             23032..24042
                     /locus_tag="PLM79_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOV85665.1"
                     /translation="MGILIILCVFALFVIFFAVKGFIIIQQSETMVIERLGRYHRTLP
                     SGINIMWPLFDKPRQIEWRYVQTDPAGKTIVRRVNLGRIDLRETVYDFPKQSVITKDN
                     VVTELNALLYFQITDPVRAVYEIANLPDAIEKLTQTTLRNVIGELDLDETLSSRDTIN
                     SRLRAILDDATEKWGVKVNRVELQDISPPPEIRVAMEKQMRAERDRRAAILEAEGMKQ
                     ASILEAEGAKSAEINKAEGAKQARILVAEGEAQARIRVAQAEAAAIKLISEAVAGTRG
                     DPVNYLIAVRYIEALKEMVSGQNNKVVYLPYEATAILGSVGGIKDLFQAIVPEGPGPS
                     RG"
     gene            24566..25159
                     /locus_tag="PLM79_04840"
     CDS             24566..25159
                     /locus_tag="PLM79_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV85666.1"
                     /translation="MDAWYWDLIEDKCYGDDQAFRLIRIESTRSAMGVERFFEAVHPD
                     NHDKIRKARNSSVEQGLPHQVECSFLQPDGSFRSVSSRAGPVRDDHGRPLRLNGILRD
                     ISEGKWAEEKREESEDRLHWARKAESLGRMAGAIAHSFNNLFSVAIGRLEPAMTDLPP
                     ASGAAEDVAEAMTALERSANLCHLTLGSLADGLRAAR"
     gene            complement(25236..26399)
                     /gene="chrA"
                     /locus_tag="PLM79_04845"
     CDS             complement(25236..26399)
                     /gene="chrA"
                     /locus_tag="PLM79_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOV85667.1"
                     /translation="MSGSNSTPTLPQLFGSFLRLGATAFGGPAMIAYIRKMAVGRKGW
                     MDGELFQAGVALCQTIPGATAMQMAAYVGLSVRGVRGAMTSFLAFGLPAFLLMLALSV
                     AYSHTHELPAVISVFSGLRTVIVAVTANATLSFGRTSLKNWRNGAVALAAAALFGIGA
                     NPFLIILLAALLGMLFDGRQPVASGNSPSGNPAHTTRPLLLLVLLSAAALAVLYALRR
                     DLFDLALLMGKIDLMAFGGGFVSVPLMFHEFVEARGWMNSSTFLDGIVLGQFTPGPIV
                     ITATFVGYLVHGLSGAILASAAIFLPSFLMLVGMAPWFDRLRSIPRFTRAIGGILCSF
                     VGLLLTVTVRFSLNVHWDWPHVVLAAAALAALVLKVDILWVVVGGTAVSAMIF"
     gene            27946..31155
                     /locus_tag="PLM79_04850"
     CDS             27946..31155
                     /locus_tag="PLM79_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOV85668.1"
                     /translation="MNRKESRFSGALGGTCLLAIVVAFLTGGAQLAAVGKVDAQSKNQ
                     PAIRELRAAAKGKIAPDLLESFQRAPGQPRRFLVYLKDQADLKGAQGLNDWEAKGNFV
                     YDALKQKARTSQQNLLGYLETQAALGRVVAFKSFFIVNAIRVEAGEEVLMELAEREDV
                     DHIEAIKAIPVPKPVVQPERRTEGIEWGVDKIRAPELWNEGVNGSGVVVANIDTGVQY
                     SHGALVQQYRGTATGSHDYNWFDPTGTFPNFPGDNNGHGTHTMGTMVGTDGGSNQVGV
                     APSATWISCKGCESNSCYDDHLLACAEWVLAPTNLSGTGADPTKRPQVVNNSWGGGAS
                     CDEWYLASVRAWRAAGIFPAFSAGNSGPDSGTVGAPGNYKESFATGATDSGDVIAGFS
                     SRGPSACPNSGIKPNVSAPGVNIRSTTPGGYESWDGTSMASPHTAGCVALLLSALPST
                     SIEGLESMLSTNAVDLGSPGPDYSYGNGRIDCYAALHGTKKGRLIAEPTSFEVTITKG
                     AVTTRQLSLQNIGEAAASFEIAEVDLGTENALGKRRADGASKKQGLPTGRLGKRAGAP
                     ELSRQDLPSAQSLGRRVPVPAAALGGGTVIRSWDPGISAPWGIAYDSKNDTVWVSSPV
                     SSWEGDGNIYEYGTDGEPTGRSWPYSWNPPYGPADAVFNLNTGTLWVMNVDSSGGKNC
                     VHEIDPGLGYTGTRICPGGTGFSTSQRGLAYDPDTDSYYAGSWNDGMIHHFASNGTML
                     DEEYVGLDIAGLAYNPKTKHLFAVVSADPGPVYVLDAANQYSVLGSFEIPGFSGYSGA
                     GLEIDCDGNLWAVDQNTRMVYQVESGEEGAGCKSSDVPWLSEAPTSGSVSIDSSVNVE
                     LTFDATTLEPATYKARLVISTNTGQDPIVVPVTLTVQEEVDIFTITATVKDGKGGSIS
                     PSGEVQVPYGSDSTFTIKPDKANGYKTVDVIVDGVSVGARATYTFSEVSANHTITAVF
                     GKIVVTAPNGGEKWKSGSNHAITWDADGDIGEEAVITLLTRQGVKHSVIAKSARIALK
                     KYKWKIPTKIPAGAYKIRISSKAHPKVKDTSNKVFKIIR"
     gene            complement(31374..33191)
                     /gene="ftsH"
                     /locus_tag="PLM79_04855"
     CDS             complement(31374..33191)
                     /gene="ftsH"
                     /locus_tag="PLM79_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOV85669.1"
                     /translation="MERHTKFSLWYVIAAVWGVLIIQDFIAAQYRPRALPYSEFLKSL
                     QAGDIIEVVITQERISGKMRVTENQQPKEVAFITIRVDPDLSTELSKHNVIFRGQPES
                     TFLRDLLSWTLPALVFFGIWYLLMKRLNPGAGMMSFGRNKAKLYAEKEVETTFGDVAG
                     ADEAKEELREIVDYLKEPGRYQQLGGRMPKGVLLVGPPGTGKTLLARAVAGEAGVPFF
                     SISGSEFVEMFVGVGAARVRELFQQARDKAPCIIFIDELDAIGKARGGFVVGGHDERE
                     QTLNQLLVEMDGFDPRVGVVILAATNRPETLDPALLRAGRFDRQVLVDRPDVNGREAI
                     LRIHAAKVRLSPGVDLKVIAQKTPGFSGADLANVINEAALLAARKNRDSVEMEDLDEA
                     VDRIIAGLEKKNRVINPREKEIVAYHETGHALVAAFTPGADKVHKISIIPRGIGALGY
                     TQQLPTEDRYLMTRQELLGKIDVLLGGRTAETLVFGDASTGAHNDLQRATDVARAMVA
                     EYGMGETLGLSTYPRQKSPMFLSPEQAPLMGKEYSEATAAKLDEEVKTLLEERALHVR
                     ELLTRNRALLEKVGSRLLQVETMDAEEFYSLVEEAGVPA"
     gene            complement(33368..34090)
                     /locus_tag="PLM79_04860"
     CDS             complement(33368..34090)
                     /locus_tag="PLM79_04860"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOV85670.1"
                     /translation="MTETKTQPSFDRRSFLRTMAGGFVVLGSGLVGLSGCSSSHSTAS
                     LTLPPLPYKLDALEPYISKRTVDFHYDKHHRAYVDKTVELVKDTGLAGLSFKEIMKKT
                     YGSKDKAAVFNNAAQAWNHEFFWNSLKPGGSKPEGEILKQIEASFGSLDNFKQEFLQV
                     AVGRFGSGWAWLTADGKKLKVSSTSNADNPVVQGQRALLTIDVWEHAYYLDYQNRRAD
                     YVKTLIDQVLNWEFAAANLQKA"
     gene            complement(34960..35205)
                     /locus_tag="PLM79_04865"
     CDS             complement(34960..35205)
                     /locus_tag="PLM79_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HOV85671.1"
                     /translation="MSKHRHTFVETYDGLIAFGMSRDVDEKSLTCYLQKFSDDELLKV
                     LVPRLSDEEINGIFEMLSQTLRNHLADREYHALFLKD"
     gene            complement(35211..36542)
                     /locus_tag="PLM79_04870"
     CDS             complement(35211..36542)
                     /locus_tag="PLM79_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOV85672.1"
                     /translation="MKRKDQIPPASGKLGVLIPGLGGAVSTTFVAGVELVRKGLAAPV
                     GSLTQLGTIRLGKRFEHRTPQIKDFIPLAGLDDLVFGGWDIYEANGYEAAMYARVLNR
                     DHLEPIKGFLSGITPMRGVFDRRFVRNLDGTFVKKGSNLRDHVEALKEDIREFKRVHG
                     ADRCVMIWCGSTEAFLEPADFHRSPESFLEALDRNESRISPSMLYALAALESDIPFIN
                     GAPNLTVDIPAMVLMAREKNIPIAGKDFKTGQTLMKTILAPGMKARMLGLEGWYSTNI
                     LGNRDGLVLDDKDSFRTKEESKLSVLEHILQPSLYPNLYKNYYHKVTINYYPPRGDNK
                     EGWDCIDIFGWLGYPMQIKVDFQCRDSILAAPLILDLVLFTELAQRIGMGGIQEWLSF
                     YFKSPMHKEGLYPEHDLFIQLMKLKNTLRYLKGEEQITHFGLDYYMDLEED"
     gene            complement(36554..37129)
                     /locus_tag="PLM79_04875"
     CDS             complement(36554..37129)
                     /locus_tag="PLM79_04875"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOV85673.1"
                     /translation="MIREEEQGPISYTTFPSPCGRILLAANERGICRLHFFGAVGPRK
                     SEIQKILHLPSIGGEDRGSSAVESLLCRARESVCGYLQEGNPLEEFPLDLSSGTDFQR
                     RVWQCLGRIPRGQTRTYLEVAAQMGSPRASRAVGQACGRNPVALFVPCHRVVGAGGKL
                     GGFSGGIEVKRNLLELESGSGNCHSRMEDKR"
     gene            complement(37122..37769)
                     /locus_tag="PLM79_04880"
     CDS             complement(37122..37769)
                     /locus_tag="PLM79_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85674.1"
                     /translation="MKIIQTIVGVMEVFCYLVYDEASGDGILIDPAGDTDRLMKLLEE
                     RGVKLRYIVNTHGHADHTCGNEELKKRTGAPIVMHATDDTFFQRPESQAFAGMMGFVP
                     SPPADVRVGDGTELSFGDITMKFLHTPGHTPGGCCILIDGHLFTGDTLFVGAVGRTDL
                     PGASFDQLIQSLKNKVLALPPQTVVWPGHDYGDRPYSSVREERETNPYITDFIDD"
     gene            37887..38945
                     /locus_tag="PLM79_04885"
     CDS             37887..38945
                     /locus_tag="PLM79_04885"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOV85675.1"
                     /translation="MSDSRELFVVEYRGGRCPSSFPGVEGLLGVWPEPPYFYCFFGRE
                     VSREEIAGFGREYGWTFRDTYRLAYDQWQDVAPATLRVGPFLLHTPGTPPPHPERAEG
                     MVLHLNPGIVFGSGVHPSTRGCLLVIGELFASFPIETVTDLGTGTGILALACARLGCR
                     RVTAMDCNPLAAREALANVRLNRLTSVIHVVTADDPATLNRGGDLLLMNIEWAALRKV
                     LEGTAWLRYGKVVLSGFLETQWGQVRDMLPAHYSIFCRRSVDGWGTVAVANPASGNTN
                     PGGSGAREPPWESSRQRFGRGAGGRPEKTGDPSNKNPGQVEPAPGFVPTANGFVKSPR
                     RDRLSGPLGSVLRRIPRP"
     gene            complement(38875..>39079)
                     /locus_tag="PLM79_04890"
     CDS             complement(38875..>39079)
                     /locus_tag="PLM79_04890"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOV85676.1"
                     /translation="ETSHAIRAAIDEARQAAPGTVIVFLYSGHGLLDLAAYDNYLRGE
                     LKDEVFSEEQIQAALKACPDVGF"
BASE COUNT         7476 a        11683 c        12136 g         7684 t
ORIGIN      
        1 cgccggtttc ggactcgagc tcccttttgg ccgactgcag ccgttgccgg gtggcgggat
       61 cctgcagctc gtcgagggac ctcagggccc ctccccggcg ccccagccga aacaggagcc
      121 tctcctccgg gggcagggcc aggtagcggt cgatcaccgc caacatggcc tctttgtctt
      181 cgggcagttt tccggccacc tcctccaaaa ggttcatgat gtgatccgag gtcaacacgc
      241 tgtgaatccc atccagggat tcgaggaacc accggatttc ccggacggtg tcgtcgtctc
      301 ccgggggaag gaatctcccc gccttcattt cccggtacaa gggggcccgg gaaggaacgc
      361 gcagacttct caggcggatg aaatgggggt cgacggcgtt gagtacccgg gcggtttcga
      421 gggcgtgttc cctgctgagt tcccgcccgc ccagtcccgg catgacgtac tccgagaggg
      481 tcatccccgc ctcgatcacg ttccgaccgg cgaggatgtg ctgttccgcg gtgcagccct
      541 tcttcaccgc ccggagcacc cggtcggagc cgctctcgag acccacgtgg atacggtcca
      601 aacccgcctc ccggatctcc cgaagctcgg cgacgctctt tcttgccagg gtgctgctcc
      661 gggcgtaaga ggtgatgcgt cgggtttggg gcactctctt cttcaagtac tcgagggcct
      721 gcaccaggag atccgtgggc agaaccaggc tgttggcgtc ctggataaag acggtgcccc
      781 tccccgaagc atgccacagg gcgacgtgat ggtagcaggg attttgagca tgccgacgaa
      841 gcgacaggct caagacctcc cgggtgatcc ggccgcctgc tcccatggaa acggaagccc
      901 ggtgaagatc ttcgagaatg ccggccaccg catcgatgtc gcggcggatt tcctcgaggc
      961 tccttcggct gaattccgct ctcttgtaga ccgggcagaa ggtgcaccgg ttccacggac
     1021 aattgcgggt gaagcgcaga aggaggctcc cggcttcact gggaggccgg atggggccct
     1081 gttcgaagat gaaggactgg gtcatggtgc cgtctccgtg ggatcgtgtc atcatagcac
     1141 gggacggtcc cggaagtcag cggggatgtt ccgccaaagc ccgcgcccat ggctttggtg
     1201 ctgcggcccg gcaggggagg accccggaag tcgggtatgc gttcgcggaa tcccaccttc
     1261 gtgaatactc cagggacctt gcgtcccctc cgccggaatc gggcggacca aagatttccc
     1321 cgaggacaga cttttggtgt caaaaccctg cgtcccgaat tgtttccggg gtttggcggg
     1381 gaaaaaacat ttgacccgtg aggtttcatg atataggata aatgccatcc tatttatgct
     1441 gctatgtggg aaaggagtcc cccatgcgtc tcactcgagc cggagaatat gccgtgcgtt
     1501 gcgttctctc cctggccggt cgaaaacccg gcgaggtggt ctcccgaaag cagatcgccg
     1561 cggaaatgga catccccgac cagttcctgg gcaaaatcgc ccagcagctc gcccgagccg
     1621 gaatcctcga aatcgtccag ggggccaggg gaggctttcg actgcgggtc ccccccgatc
     1681 gattgagcct cctggaggtg gtggaggccg tcatcgggga aatcttcctg aacgattgcc
     1741 tcatgaggcc cgaatcctgc ctgcgaagtc ggtcctgcgc cgtgaaccag atttgggaaa
     1801 aggccaggga ccagttgagg gccaccctgg gggagttcac cttctccagg atcctggaaa
     1861 cccagtgctg cctggtgccc cccggggaac cgggcgaaaa tcccccctaa aggaggacac
     1921 cccatggatc ccttgattct ctcccggctg cagttcgccg cagccaccat gtttcacttc
     1981 ctcttcgtgc ccctcaccct ggggctttcc atcctcgttg ccgtcatgga aacccgctat
     2041 gcacgaacgg gcgaggagat ctacctcaga atgaccaagt tctgggggaa gctctttctc
     2101 atcaatttcg ccctggggat cgtcacgggc atcaccctcg aattccagtt cggcaccaac
     2161 tggtcccggt actcggcttt cgtgggggat gtcttcgggt ccctgctggc catcgaggcg
     2221 acggcggcct tcttcctcga gtccaccctc atcggcgtgt ggatcttcgg atggaagaag
     2281 ctctcggcca aggcccacgc cgtggtgatg tggctcatcg cgggcgcctc gaccctctcc
     2341 gccgtgtgga ttctcatcgc caactcctgg atgcagcacc ccgtagggta cgtgatccga
     2401 aacgggaggg ccgaactcga gagcttcagc gccgtggtgt tccaggaatg ggcgctcctc
     2461 gagttcttcc acaccgtggc gggggcctat atcctgagcg ccttcttcgt catgggaatc
     2521 agcgcctacc acctcctgcg aaaccgccac gtggacttct tttcccggtc gttccgcatc
     2581 gcgctggtct tcggcctggt tttttccatc ttcgaggtga tcgagggtca cgggcatgga
     2641 gcggacctgt acgagaaaca gcccaccaag cttgcggcca tggagtccca ctgggagacc
     2701 tcgacccggg cgcccatcca cctcatcgcc ttccccgatg aaaagaacga agggaatgcc
     2761 gtggcgctcc tgcccatccc gggagccctg agcatgctgg ccaaccattc cttcgacgcg
     2821 gaggtaaagg gtctcaagga ctttcctccc gccgaacgcc cccccgttct catcaccttc
     2881 gtggcgtttc gagtcatggt caccctgggg gtctttttcg tcctggtgac cctttacgca
     2941 tggttccgac gagaccgcct cccggagagt ccccgcctcc tgaagctgct ggttctctcc
     3001 attccccttc cctacattgc caaccaggcg ggatgggtgt tggcggaagt gggacgccag
     3061 ccctggatcg tttacggcct gatgaagacc tcggacgccg tttcccccat ccacggctct
     3121 caagtgctcg tgagcttcat cgcctttctc gtggtttacg gaatcctggg atttgccgga
     3181 ttctatctca tggtgaaaca cgccaagcag gggcccgtcg aggtgccgca gggctagggg
     3241 tttcatcttt cgcacgcttt tcctgcgccc ttgggcgcag ttaggaaagg gggtcgtcat
     3301 gtggttgcag gatgtatggt tttttctgtg gggacttttg tgggccatgt acttcatcac
     3361 cgacgggttc gacctgggaa tcgggtccct ggttcccttt ctggggaagg aggaagcgga
     3421 tcgaaaggtc atgctccatg ccatggggcc agtctggaac ggaaacgaag tgtggctcct
     3481 cacggcggga ggagtcacct ttgccgcctt tcccaaggtc tatgccgtca tgttctcctc
     3541 cctgtattcg gcgctcatgc tcgtcctgtt tgccctgatt cttcgagggg tgtactttga
     3601 attccgagga aaactcgatg atccccgttg gcgaaaggtg tgggacgggt gtctgttcct
     3661 gggcagcgtc ctgcccgccg tgctcctggg agtggccttc ggcaatatct tcaaagggat
     3721 tcccttcgat gaaaacgggg tgtaccacgg ctccctcttc accctgctca atccctacgg
     3781 actcctgggg ggcgctctgt ttcttcttct cttcctcgtc cacggggccg tctacctggc
     3841 cgtaaagagc gaaggagacc tccaccggag ggccgtggcg acggcgaacc ggctgtggcc
     3901 ggtgctcctg gtggctgccg tgttcttttt tgcggccacc caggtgcaca cgcgactcta
     3961 cgacaactat ttcgccaaac cgatcctgtt cctggttcct ttagtcgccg tggcagggct
     4021 tttggggatc aagatgtttc tccgcaaaca ggccgtgggg aaggcctgga tttcttcggc
     4081 cgtcaccatc gccggggccg tcttcttcgg agtcatcggg ctctatcccg ccctgtttcc
     4141 ttcgagcatg gccgcagggg ccggcctgac ggcccacaac gcctcatcga gtccccttac
     4201 cctcagcatc atgctggtgg tggccctggt cttcgttccc atcgtcctgg cctatcaggg
     4261 ctgggcccat catctcttcc gaggaaagat cacttccggg gacccggcct acgaggaggt
     4321 gcactgattg ggggaaaggg cctgaaccca tacgcccggg tccgtaaagg ggctcttccc
     4381 caagatgagt ggttgttccc gttgtatggg tgtgcataga gcccgctccc gggcctgcag
     4441 gggagccgtc ctggaggagc tgcgccccgt ccccgcccgc cgggagcgcc gccggatggg
     4501 aaatgaccat ccaagatttc tcggtcgggt cttcatggcc ccccccggcc cgtcgcgtcc
     4561 cgggtgggaa gaaggtaatt cctccgccga gtgtggaaaa gcggggtccg cccgtcgagg
     4621 gagaagagac ggcttcctct ccccgaaggg gagacggctt gggggagcgg gcactcttcg
     4681 ttgcggcccc ccggcccgga gggaccttcc ccaaacctct tgccccgttg ccatttttga
     4741 ggcaaaagag gagaccgccc ttggggcggt cggaaaaatg cagcggaggt ttggagggat
     4801 gatccagctg tcggaggtcc aaaagcaggc cgtcgagtac atgggcaagc cgaccctggt
     4861 cacggcgggg gcgggttcgg gcaagacccg ggtgctcacg gccaagttcg cgtatctcat
     4921 ccggataggc tacgatcctg cgcggatcct cgccattacg ttcaccaaca aggcggccaa
     4981 cgagatgaag gaccgcctgg agaggctgac gggactcaaa agcttccagt ttccctgggt
     5041 gaggaccttt cattcggcgt gcctgcgcat tctcaaggag cactgccttc gggccggata
     5101 ccggcatccc ctctatgttt gctcgaccta ccaccaggag aagcatatca aggccctgct
     5161 ggtggaaaac ttcaacctgg accccaagaa gtaccttcac ccggttgccg ccgccatttc
     5221 ccgggccaag aactcgggac ggccccggca gtacctggag ggcatccggg tggaccgtcg
     5281 catccaaaag agcctccccg acatctacgc cgagtacaac cggcgactca aaggggccaa
     5341 ctccgtggat tttgacgaca tcctcctgag gacccgggac ctgctggatc aacacgagga
     5401 ccttcggcgc cgttatgccg accatttcca gtacgtcatg gtggacgaat accaggatgt
     5461 gaacgacctg caggaagaaa tcacccgcct ggtcctgaaa gacggcaatc ttttcgtggt
     5521 gggggatgat taccaggcca tctacggctt tcggggggcc aatgtgggga atttcctgga
     5581 ctttcgccaa aggcacgccg acgccgtcat tttcaagctc gaagaaaatc accggtccac
     5641 ccgggaagtg gtcgagaccg ccgagcttct catccgcaag aatcggcaga tccccaagaa
     5701 gtgtttcagc cggaagaccg gaggaagcgt ggaggtggcc gccctcgaga acgaatttgc
     5761 cgaagcggac cgggtagtcg acctcattcg gcatcaccat cgctcgggga ttccgtacca
     5821 ggccatgtcg atcctgtatc gcagcaacgc aatttccctt cctttcgagc aggaattgag
     5881 ggccaacggg gtgccgtaca tgctcaaggg cgacaaggga tttttcgagc gcaaggaaat
     5941 cctggatatc aactgctatc ttgcggctgc ggcgaatccg cgggacaacg tgagcctcga
     6001 ccgcatcctg aacgttccca aacgcggcat tggttcctcg gcctaccgga aaatcctgga
     6061 cctcaagcac accgacatgt ccctccggga cgcctgccgg gaagccgtcc gcagaatggt
     6121 tctcccgacc aaggtccttt tagggttgga acgacttctc gattttcttg aatccatcgg
     6181 caaagaaaag cccgaaaagg ccatcggacg agtcctctcc gaagccggtt atgaaaccta
     6241 ccttgccgaa tactgcaaag gtgagaacac ccgcgaattt cagaaccgca tggaaaacat
     6301 ccagtttctg ctctacgcgg cttcacaaaa acaggacctg gtcgagtacc tcgaggaaat
     6361 caccctggac gggagtgacg aaaaggagga aaaggatgaa tccaagagcg gggtgaccct
     6421 gtccacggtg catggagcca agggtctcga gtgggaagtg gtctttgtgg tggggctcga
     6481 ggaggatctc ttcccccact ggagggcgct tgcccaagac tcggccgacg gcatccagga
     6541 ggagcggagg ctggcctacg tggctttcac ccgggccatc cgccatctgc acctgtcctg
     6601 ggtgggcgtg cgaaagggcc agagtcgaaa tcccagcgtc tttcttaagg aggcgggact
     6661 cctgtgagca gggggcgcgg gcggccaatg ccgggggtga gcgcgcccgg ggggatgcag
     6721 gagcgaagaa gtgcacaccg gaaaaacggg gagcttccgc aacggcggga tgagtcccgg
     6781 caagtccgga tgcccgcctt tagggtcccg ggtggacgcc gatggggggt gtgccccgga
     6841 atctcgaagg aagcccggtc agcggggttc gagtccgcca cggccgtttg ggacggggta
     6901 cggtccgctg ccgtacccag tttgggggga acaacaaccg aggcggggcg ccggaccggt
     6961 gcccgcacgc cggcaaagga ggggtttatg ttacagggtc gttgtggctc cgggtggttc
     7021 cggggtcttt tgctgttgtc ctttctcccg gcggctctcc tgccgtctcc ggggttttgt
     7081 gccgcacctg cgccccggtt tgaagttccg atcaatcaga aggcttcgga cattctgccg
     7141 ccccagttcc tttcaggtcc tcactttcga gtccgggagc gggtggtttc ctacggatac
     7201 atgcaccatt tcaccgtgga ttcggatttt ggagtgttcg agaccacggg agatttcgcc
     7261 ctgcgaaagc tggtccggga aattggggcc atcgcagccc tgcaccaggt gaaacgaggg
     7321 gaggcctatg cccagggatt gaagaaagcg gcttcccagc ctctcgaatt cggggccaac
     7381 ctggtcaccg atcccgtgga caccatcagc ggaatcccaa agggaatcaa ccggttgttc
     7441 gaaaacgtga agacggggct cacctccaag tcccaaccgg gcgaggacgg caaagtggag
     7501 caggccctgg cggtgtcttc caacaagaga gagttggccc ggcgattggg agtcgacgtc
     7561 tattcgagca acagcgttct gcaaaaggag ctcaacagcg ttgcctgggc cacgaccctg
     7621 ggatccctca ccctcaccgc cgccctggca ccggtggggg gaccggccgt ggccgccgtc
     7681 tcggccacca ggacggccca gcaggtgagc gacatcgtca atgaatatcc tcctcaaagg
     7741 ctccggcaaa tgaacgagga gaagctccga accatggggg tgtctcccgg cctcatcgca
     7801 caattcctgg atcaccccgt ctataccccg acccacgaca cggtcattgt gggatgcctc
     7861 gatgctctgt cgggcgccaa gggacgcgaa gccttcctgc agctggccct tgccgccgac
     7921 ggggaagaaa cggccaactt ctttcagaac atggcggaaa ccatgaggta ctaccacctg
     7981 aaggtctccc ccctgcggga gatttccgtc tacggccctc tggctttttc cgaggccgcc
     8041 aacggcacga ccctcatctc ttttcccctg gatcacgggg tgtggacgga acgggccgcc
     8101 cagcgggttc ccggggtcat atccgcttac aagtccgccc atccgagatc caagaacctc
     8161 gaggtctggg tgacgggaac cctgagtccc atggcgaaaa aggagttgaa caagctggga
     8221 gttcgggtgg tggaaaaggt gtccgggcag atcgaatttg tctactaggc ggggtcctgc
     8281 agccgtttac ggacgggggg agggaacggc tcctccggga tattgagtcg aatccctccc
     8341 cgaccgttcg ggtccccatg gcaggtcctg cccgggaaaa cccgtgaggg ggaacttgcc
     8401 ctcacgcctc cagttccacg gacaccggta ccgggtgact gacgatgtcg aatacggggg
     8461 atcgtttcct ggccaattcc aagagctccc gcaattcctc ttgggaggcg tctcccttga
     8521 tcttgaaggt gacccggatg ttccggtatc cgttcctcac ctcctccgac atcccgagaa
     8581 agccccgtac gtccaggtct ccctccaacc gggattccac cttccggagc cggattcccc
     8641 gcaccgccgc ctgggcgatg agactcgtgg tcaggcagcc cgaaagagcc gtaaggacaa
     8701 actccaccgg gttggctccc aggttctttc ccannnnnnn nnnnnnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnctttcat tcccgaacgt cggagcacaa atccatggat gaaaaagtta
     8881 ccgccattta cgtcatggcc cgaaaagacg aactgaagcc ggaacacggg gggtcttacg
     8941 atacgctctt gaaaagccag gaggaagaga tccgggagtt tctccggctg aaaacgggcg
     9001 ggaatcttca ggggcgggtg gaggtctaca ctcgaagagc gcagctcctg atggatatcg
     9061 agcgcagccg ggttcgacgc ctggccgtcg agagcgtgga acggctgggg agcagcaggg
     9121 aagagatcga agggattctt ttcgagctca aaatggaagg ggtcgagctc ttgagcgtcc
     9181 gagacaatcc tgcgtgacga cccgggagat agggaccttc catggaagaa ctcgccaagg
     9241 accaaccgtc cacggttatt caagggggtt cccacggcct cgtggccgac ttcgaggaac
     9301 gacggcgagt cctggatccc ggcacgtcgt ttcacctgga ggccccggcc ggatcgggga
     9361 aaacgaccct gctcaccgcc cgtttcctgg ggctcctggc caccgtggga catccccggg
     9421 aaatcctcgc cctcaccttc accaacaagg cggcggcgga aatgcgggaa aggatcagcc
     9481 aggtgcttta ccgcgccggt gaagggaagg aaggggacaa tccctttgag gtcgctctcc
     9541 tggattcggc ccggagggcc ctcgaacgcc acgcccagtt ccgggagctt ctattccggg
     9601 gccagtttct ccagatccag acctttcatt ccttttgctt tcacctggtc acccaggcac
     9661 ccctcgaggc cgggatcagc ccggggtccg ttctcatgga cgaagaggaa caggggttct
     9721 tcctgcggga agtcatcgac gaggtcctcc gtggtcttct ccgtgcttcc ccgggcgatc
     9781 ctttgcggga cgccctcgaa gaccggctcc tgtattggaa caactcgtgg tcgtgcctgg
     9841 cccgggaatt ggcggagctc atggagcggc gcgagtccct aggcgaactc ctgggattcc
     9901 tggacctgga aaaggccgcc gattatgtgg accggtggat cggacagctg gtggaaggaa
     9961 aactcgccgt tctgagaagc gggttcgccc gatcctccct gggacagggg tggtccgatt
    10021 ttgtgagtca cctggaatcc cgaggtgccc ccgcggccga actgttgccg gttgcgctcc
    10081 ccgagccccg ccgggagtcc ctggaatcct ggcagaagat cgccgacgtc cttctcaccc
    10141 aaaggggtgg ggtgcgaaag tccgtgggcc ccgcctcggg gttttactcc ggctttgcca
    10201 agacctcctg gtttgaagcc atgcattccc tggattccga caccgtcgcc cggctctccg
    10261 aaatccggga tctccccgcg gaggaatccg ttcgcctgga cctggaaccc ctgtgggacc
    10321 ttcttctcct taccggtgag gtgatcaagg aatacgaaga ccggtgccga gcccggcgag
    10381 tcctggattt ttcctccctc gagaccgccg ccctgcgcct tttccgtcag atccggccct
    10441 cggacctcga actgtatctg gaccaacgga tacaccacgt actcgtggac gaattccagg
    10501 acaccagtcg acagcagtgg gagctcctcc aggggctgtg tgccgaatgg acccccgacg
    10561 gcgggaaaac ccttttcctg gtgggggacc ccaagcagtc catctacggg tttcgcaagg
    10621 cggaagtccg gctcttctcc gacgcccgtg cgggtttgcc ccttgcggga acgggccaaa
    10681 ggcttccggt ggaacccgtg attctgcgga ccaatttccg ttcccatccg agtctcatcc
    10741 actggagcaa ccgcctgttc tcgaagaccg tcatggaagg ctccgacggg caggccgggg
    10801 gagtcgaatt catgtccgcc gtgcccgccc ccgaggtccg ccgcaccctg tcttcataca
    10861 accggctggc gctctttgtg ccgtaccccg attccgtttc cgcccggcaa cgagaggccc
    10921 gctggatggc ggaccgcatc tttggagagc tctcttcggg ggattctcgg ccggagatcg
    10981 gcatcctgct ctttacccga acccacctgc ccgtctatct cgaggccctg caggcccggg
    11041 acatcgccgt ccgggttgcc gaagggatgc ttttgaagga acgcccggaa gccctgtacc
    11101 tgtggcaact gtgccgcgcc ctggtcctgc cccaggacca cctggcatgg gcctctcagc
    11161 tgcacagccc ctggctgacc ctcggatacc gggagatcct ggagatctcg aaggaagagc
    11221 cgaccccctg ggtcgaaaag atccgggtcc acggggaaaa gaatccccgg gtgggagagt
    11281 tttgggagcg gctgctgtac gcccggcagc acctggggca tgagcccctg gccgacgttc
    11341 tggaaaggac ctggctggat ttgggaggac ccgaggccgt gggagagcga tggggcagcc
    11401 ggggccttgc ttcctccttt cgactcctgg agctcgtccg ccgggcggag gtccacgaac
    11461 cggtccagac cctcgatcgc ctcgaagagc tcctggagag ggcctacgaa cccatcgacc
    11521 cggatgcggc ccagtccacg gtctccatga tgaccgttca ccgggccaag gggctcgagt
    11581 ttgactccgt gtaccttccc ttctgcgact gggatcccct ggcccgggag cggggagacc
    11641 gaccggccta tctcctcgaa cgcgccgaag gaacggacgg gcggatgctg cttgcggtcc
    11701 atccggaccg ccgcacgggg gaagtggatc caacctaccg gctggtccac cggctgcgcc
    11761 tggaccggcg ttggcaggaa gccaagcggc ttttctacgt ggcggtgaca cgagccggga
    11821 gccgactgtc tttgagcgga gtcttgagcg gcggtcgacg ggaggggcct ccgagttttc
    11881 cggcccggac tcccctgggt tggctcaacg accactatgc cctcgaggag aacctggact
    11941 tggcctcccc ggatttctcc gccgtcgggg aagggaacgg acccgccggg tctcccgcgg
    12001 ctgccgccgg gaaaagggag tgggagagcc gggagggaga ctttcgagtg tgggtcgagc
    12061 ctgctcccga gtctgcggaa tccgggctgc cgtcgggacc tccgttgccc gagatccggc
    12121 cggcggaatt cttccgggag cggcctgtct ttcgggttcg cagtccttct tccctggtgg
    12181 gtgccaaggg ggaaggaatc gaagtcgcgg aggcgagctc ggacggtctc ccggtcgatc
    12241 ccgccctgtg ggggaccctc atccatcgaa ttctcgaaac ctggggccga gaggccacgc
    12301 tgcccgatgc ggcccgggtc cgcagctacc tgcgactcga gggcatggac gagaaggatg
    12361 ccgaccgatg ggcccgggag agcctcgaag aggttgaggc ctgcctcgag gacccctttc
    12421 ttcatcggct gtattccctt ccttccggcc agaggtgggt ggaatgggcc ctcgagtctc
    12481 cccgttcgga gagggtcctc gacgtgggag tcatcgatct cgtgtgccgg gacggcgatg
    12541 cctgggtcct ggtggatttc aagacctcga ggccttccga ctcccaggat ccgaatgcct
    12601 tctgccggga cgggttgatg cgataccggc cccaggtcga ggcctatcga ggcctgtggg
    12661 cccggtggaa gaaaattcgc cccgaagaca tccaggcagg catttactgg acggccttgc
    12721 ggcgatggga cccttgttga cggacggtac atggtgaatg cgaaacatgc ctttttgctg
    12781 ggcgggactc acagcgggtg cggaaaaacc acccttgccc tggccttgat ggccgccttc
    12841 gaggcaagag accttcgagt tcagcccttc aaagtggggc cggacttcat cgatacgggg
    12901 ctgcacggtc gcgtggccgc ccgagagtcc cggaacctcg acggctggat gctttcccac
    12961 agctataact gcgagctttt ccaagagagg atggccgggg cggacctggc cgtggtggaa
    13021 ggggtcatgg ggctctacga cgggtacgac ggcctcaccg aggcgggaag cagcgcccag
    13081 atggccaaac ggctgggcct gccggtgatc ctggtggtgg acgcctcgag catggcccgc
    13141 agcgcggcgg ccctggtgta cggatactcg aagttcgacc ccgatttgaa cctggcgggg
    13201 gtcgtcttca accgtgtggg ggggaaggga cacctcgagt acctcaagga agccatggca
    13261 tcgagccttc cccgggtgac cgtgttcggg ggaattcccc gggaggaggc cgtccgcatt
    13321 cccgaacgcc acctgggcct cctgacggcg gacgaagccc agctgacccc tcaatggcgc
    13381 tcgaagctgg tggaattggt ggaacggcac ttggacctgg atgaaatcct gcgaaagagt
    13441 gtttattccg gccccgtgac ggcgggagaa aaggagaaac ctccccgttc cccgaggccc
    13501 gtccccatcg ccgtggcaaa ggacgccgcc ttctgttttt actatgcgga caatttcgac
    13561 ctgctgaccc ggtggggagc cgacttgcgc ttcttttccc cccttgccgg ggaaaccctt
    13621 cccccgggct cggcgggact gatcctcggg ggtgggtacc cggagctctt tgcgtcccgg
    13681 cttgccggcc aggggaggtt tctcgccgag gttcgccgag ctgcggcggc aggcatgccc
    13741 gtctacgccg aatgcggggg gctcatgact ctcagtcgat ccatcgagac gctggaaggg
    13801 gaggttttcc ccatggcggg aattctgccc tttcgaacga gaatgctttc ccggcgcaag
    13861 gcgttgggct atacggaagt ggaactccgc agcagttgcc tcctggggga accgggacag
    13921 gtcatccggg gccatgaatt ccactattcc gaaattgcgg acatggacga gggtgaatcc
    13981 ctcgagaggg tgtacgagat tcggggccga aagggatccg agccccggcc cgaagggttc
    14041 gcgacgggct ccgttctggc cagctacatc catcttcatt gggggagctc ccccggggcc
    14101 ccccggcatt tcacccaacg gtgcaggcaa tttgaatcct caagaaggga acgaaggttt
    14161 tgagttcgaa agccaagagc ctcatgtttc tcggaaccgg ttccgatgtg ggaaaaagca
    14221 tcgtgaccgc cgcattctgt cgcatcctga aacaggacgg ctatcgagtg gccccgttca
    14281 aggcccagaa catggccctc aattccttca tcacccccga agggggagag atgggccggg
    14341 cccaggtggt ccaggcggaa gcggcgggca tcgagcctca cgtggacatg aaccccgtcc
    14401 ttctcaagcc cacctcccag atggggtccc aggtcatcgt tcggggcaag gccgtgggga
    14461 atttttctgc ccaggaatac taccggtaca agaagaccct ggtgcccgtg gttcgggaat
    14521 gcttccaacg gctggcgagg gaatacgacg tggtggtgct cgagggggcc gggagcgccg
    14581 tcgagctgaa cctcaaggag cacgacctgg tcaacctggc catggcggaa atggccgacg
    14641 ccccctgcat cctggtgggg gacatcgatc gggggggcat cttcgcttcc ctgctgggga
    14701 gcctggccct catgaccgag gccgaacagc aacgaatcct ggggttcctc gtaaacaaat
    14761 tccgtggaga cccccagctg tttcgatcgg gaatcgagct tctcgaggag aaatccggga
    14821 aacccgtctt cggagtgatc ccctattttg atcacatctc catccccgaa gaggacagtg
    14881 tggctctcga tagaaggatg aaggggagag gcctcccggc gggagaaggg cgccttcgta
    14941 ttggagtggt gcgccttccc tacatctcca actacacgga cttcgactgc ttcgaacagg
    15001 atcccgacgt gcaactggaa tacctggacc gcccgggaag agtgttccgg ttcgatgcgg
    15061 tgatcctgcc gggcagcaag aacaccctgg acgacctggc ctttctcaag cgttcgggac
    15121 tggccgatgc cctggtagcc ttttataagg ccggggggac ggtggtggga atttgcgggg
    15181 gataccagat gctgggcctc cgagtccggg atcccctggg agtcgagagc ggactcgtcg
    15241 aggtcgccgg actgggactc ctcgaggtcg agacggagat gtttgctgaa aagatcacct
    15301 cccaggtcga ggcggagatg ctgttcggct ttgggagaga ggcgccttcc ggtgtccggg
    15361 ccccggtcct ttacggctac gagatccaca tgggaagaac gagccccgtt cggggaagcg
    15421 gccggcccct cttccgaatc acccggtgcg ggggccgccc ggtttccgtc ctggacgggt
    15481 ttgttcagcc cgaagggcgg gtctggggaa cctaccttca cggggttttt gacaacgacg
    15541 agttccgaaa gaggtttctc gaggacctgg ggacacgctc ggggcgggaa gatgtgatgt
    15601 cgtccacgtc gttttctttc atgcggtgga aggaggagca gtacgacctg ctggccgacc
    15661 acctccgcaa gcacgtggac gtcgagaaga tctatgggct gatcggactg gagaaatcgg
    15721 gtcgaaatca ctgaagggat gattccattg ctgttctttc catggcactt gatggctggg
    15781 tacgggttgg acctgatcct gggagacccc aggggatggc ctcatcccgt tcggtggatc
    15841 gggtttctca tcacgaggct cgagagagtc ttctaccaga actcacccga cgcggttttg
    15901 cagcggcttg cgggtttctt tttctggacg gccgtggtcc tggtgacggc ttcggcgacg
    15961 gtggtactcc tgggaacggc ctatttcatc cataaaatcc tgggacacct ggtcatgatc
    16021 tggctggtct acagtaccct ggcaacccgg agtctccata aggaatcttc caaggtcgtg
    16081 gaagccttgg ggcgggggga cctggacctg gcccgggctc gcctggcata catcgtgagt
    16141 cgggacacct cggccctggg cgaaaaggac attcttcggg ccgtgttgga aacggtttcc
    16201 gaaaacatct ccgacgggat cgtggctccg ctcttttacc tgggactggg agggccggtg
    16261 ggcggcatgg cctacaaggc cgtgaacacc atggattcca tggtgggata cctcaatgat
    16321 cgctatcgcc atttcgggtg gtttcccgcc cgggccgacg atgttctcaa ctgggtcccg
    16381 tcccggctga gcgggctcct catcctgggt gcggcggccc tgctcaagct ggattggcga
    16441 agctcttggc gcatcctgcg aagggacgcc cgcaagatga aaagtcccaa cgccgggtac
    16501 ccggaatcgg ctgcggcggg agccctcggg attcaactgg ggggaaccaa cctgtatttc
    16561 ggaaagccgg tggaaaagcc taccttggga gatccccttc gacctctttc cctcgaggtc
    16621 taccggtcca tgattcggct cctgtacgtc tcgtcggccc tggcctttct cgcggccctg
    16681 gccgcgcaac tgggcctcta tggaatcccg gccgccctat cccccatgtc tctcctcaag
    16741 gaatggttcc tatgactctc gctcacggcg gaaatgtgta cgaagtggct tctcggctgg
    16801 gctgctcccc cgaggccatc ctggattaca gtgccagcat caaccccctg gggccccctc
    16861 ccggcctgtg ggaccatatc cgtccctcgt tccggcgggt ggaacactac ccggacattc
    16921 acaatcgggg cttggtgcag tccctggcgg atttccacac ccaggatccc cggcgggtcg
    16981 tggtgggcaa cgggtccacg gagctcattt actggcttcc tcgggccttt gggctgcgaa
    17041 aagccctcat ggtgctgccg gcattcggcg aataccagcg ggccttggaa atccaagacg
    17101 tcgagctcta caagatcttc acccggccgg aagacaattt ccagccgacc gtggaagacc
    17161 tcgagggggc catggaaaga ttctcccccg acgcccttct tctcaccaat cccggaagcc
    17221 cggcgggtac ggtgctgccc gaggaggttc ggcagtgggc cctggaaaga tcccggaagg
    17281 attcgttcct gtgcgtggtg gacgaggtgt tcgtggactt ctgcgaggag gaatccctca
    17341 aagggcacgt cgagaagctg gatcggctgg tcgtgattcg ctccatgacc aaattctacg
    17401 gggttccggg gttgagaatc ggctacctgt ttgcttcccg aaaactcgcg gaaaggcttc
    17461 ggggatttgt tccgccctgg tcggtgaaca ccgtggccca ggttgccggg gaatactgcc
    17521 taaaccgacc cgaataccgc ctcgaaaccc tttccctcat tgaggtcgag cgaacgcgca
    17581 tggcccgggc cctgggcgaa ctccccggct gccgggtttt tcccggcgcc gccaattacc
    17641 tcctcctgag gcttcccgag acctttgcgc cggctcgggt cttgcgggac gatctgctgg
    17701 cctcccaccg ggtgctggtc cgggattgca gctcctttga aggaatggac gaccggtatg
    17761 ttcgcctggc cgtccgtcta cccgaagaaa accggaaagt tcttcagggg attgcccaat
    17821 ggtggcgggg gcaagggaag gtcgggggca ggggctgaag cttccgacca ggggggattc
    17881 caaggggaca cgcgcttttt cgaccggccc cgaaggctct tttccggaaa atctcccgcc
    17941 gtcccgccca atcgggctct cagttcctgt ccacccgtcg accgaccggg cttccaaaag
    18001 actcccgaac tatggaaaag atttcacatg aaccctccga gcgacgcccc ggaggaagtg
    18061 ataaaataca caaatgtgct tgacaggatt ggcggcacgt gctagcagaa aaacgtcaaa
    18121 aaacgaattt ttgacgtcca tccttaattg cggcctcaaa acaccagcaa acaactagaa
    18181 agagccaaaa acgatgaaaa cactactgaa atgtgccatc gttgtggtgg cgttgacgac
    18241 ggccctgagc actgtctatg cacaaaccgg cagcctgatg atgcccgacc tctgcaattt
    18301 cgaggagtgg gcccagcaag tcaagaaccc cacgccttgg ttgacgtggg gtgccgactt
    18361 tagggtccgc gacgaatatt tgcccaacac ggtcaccctc accgacaagg atccgctggc
    18421 ggaacagaac gtggcacggt ttcgggcccg ggtatggggc accgccaaga tgcccagcga
    18481 cgtgagcttc aatgcccgcc tttcggcgga accccggctg ttcaccgagc cggccggttt
    18541 cggcccccga aaaaccgcca gcggcatgga gtggcgttac gggatcctgg acaatgtgaa
    18601 tctcaagtgg aacaacgcct tccagcagcc tttgaccatc accgtggggc gccaggacat
    18661 catgctgggg gatttctatg actggtggct cgtggccgac ggcaccccgg gcgacggttc
    18721 gtggaccttc ttcctggaca gtgtccgcct cacctacgat gccaagaaca tcgacaccaa
    18781 gtttgacgtg atcaccatcc ttcagcatgc ccgggccgac gagtggatcc cgaccatcgg
    18841 aaacccgacc tacagcgact ggccgcgctt caacgacacg gaacatcccc tgaccgaaca
    18901 gaacgaaacg ggagtgatcg cctacctgtc caacaagtcc ctcaagaaca cggccatcga
    18961 cgcctatttc atctacaagc gggacgacca ggaaaccatg gagcgcgggg gcttcccgtg
    19021 gattcccggg gacaatgcgg acatcttcac cttcggcggc aagatctccg gcaacataac
    19081 gcccaactgg cagtactcct tggaaggcgc ctaccagttc ggaaacaagg aagacaggat
    19141 cctgggagtt ttcgccgatc gcgacatctc ggcctatggc gccaaggcca agctcaccta
    19201 cctgttcaag gacaagctga acaaccagtt gttcgtgtcg ggggaattcc tgtcggggga
    19261 tgatcccagc accccgggaa ccgacgaaat gttcgacgtg ctctggggcc gctggccgcg
    19321 ttggagcgag ctctacatct attcgtacgc aggggaaacc agcggcaaga tcgcccagat
    19381 gaacaacctc atgcgcttcg gaccctgctg gaccttcaat cctaccaagg ggatgacctt
    19441 cagtgtggcc tataatgcct tgtttgccca ggaagaagtg cccacgcgcg ccgttgcccc
    19501 gaggttcagc aataatggag acttccgagg ccacttcctg cagacggtgc tcaagcacca
    19561 gttcaacaag cacctcagcg cgcatatttg ggttgaattc gtctggcagg gggactacta
    19621 cgctcggcgc gacgtgatgg ggtttgtccg tccggaaatc ctgttcacgt tctagaacga
    19681 tcccattcgg cacgacccgg ccgatccgcc cgcgggatcg ggcctcgaag ggtgcaaaga
    19741 aaccgcccca aaatccgctc aacccgccgt ttcggcgaac ctcgggccgg atccggggtt
    19801 cgcccggctg cacaattccc cgcgaaccgg attccggagt atccccgggc gacccggcct
    19861 ttcgagatcc gggcgaaacg atcgcccacg cgcccggctc ccggcccggg tcgggaatcc
    19921 cgggagggct catgcgccac cgccttgtcc cacgccgcga agacagcctg ctcctggtca
    19981 tcgatatcca ggaagccatg ctcaaggtta tcgacccttg ggaggaaccg gtccgaagga
    20041 ccaatcagct catcgaggcg gcaggaatcc tggggattcc cgtcctggca acggagcagt
    20101 atcccaaggg actgggtccg acggtcccgg aagtggcgaa aaacctggga ccctcggcca
    20161 tcttccccaa ggagcatttc agcgcctgcc tggaggaggg ttttctcgat cgggttcggg
    20221 aattcagcag gtcccagatt gtggtgaccg gcatggagac gcatgtgtgc gtgctgcaaa
    20281 cggggatgga tctcctggcg aacgggttcc aggtgcaagt ggttaagaac gccgtggcat
    20341 cccggttccg ggaagactac cagacggccc tcgatctttt ccgacaagcc ggtgccgtgg
    20401 tcacttcggc ggaaatcgtg atttttcagt gggctcgccg gtccaacacc gacgagtttc
    20461 ggaaaatcct gccggtggtg aaatgagacg cttttcgtcg ggctctcgtt cgagaacgac
    20521 ggaggcgttt tccaaccatc cggggagcgg ttggcgaagg gtattttcaa ccccggctgg
    20581 gctcacggtc cccgcacgag tacccgcacc ttccggtcgg attccgtata ccgcctctct
    20641 ctttctactc ttccgcaatt ccctcggatg atcccggcga cttcacatag ctcctggatg
    20701 ccttgatggt gacgtccctt ttgatcgtca ccgtaaagcc ggcaggagct ttccagccgc
    20761 cgatctttcg gaactgcacg gagtgctttc ccaccgggag gttggggacc gtatccccgg
    20821 agtccttcca tgggagggta tccaccttcc acttggctcc cgcggccacg gcccccttcg
    20881 gggtgatggt caccaggagg gagcccgttc gcaaaacata ggtgcctgtt accgccttgg
    20941 tccggtcctg ctggatggaa accgtctttt tggccggttt ggtccaccct ttgatattct
    21001 tgaagtagac cgcgtgcctc cctgcttgga gccccgaaac ggtggcgccg ctctccttcc
    21061 aggtcccgtc gtccaccttc caccgggcgc ctgccgcaac ggccccgttc ggtttcaggg
    21121 tcaccttcag ggaacccttc tgcatcacgt aattgcctgt gcccgaggtg gtctggtttg
    21181 cggcaacggc caggttctga gcggccgggg tggtccagcc cgcaagggtc ttgaaggaaa
    21241 cgacgtggct gccgacactg agaccggaga ccgtggcgcc gctcggttgc cacacccccg
    21301 catccacgtt ccagcgggcg ccgtcgtcga cggctcgcgc cggggagagg gccatggtga
    21361 gggaacccga cgacgggatt gttttgtacg aggtcctgtt cccggccgcg tcgtagccgt
    21421 actcgatgat cgtgccgtcc tcgtattcca cccgggtgag ccggttgagt tcgtcatagg
    21481 tgtaacggac ggtggcggcg aaagcaaagg acgtccccaa cgagaacagc aggaacgcca
    21541 gtacgaaagc tcgggtcttg ttcatggcgg ctctcctccg ggtcgagatt gaagctcggg
    21601 tcttgccgat cccggcctac catccttttt ccgggcgtgg ggtcgaagtt ccttcctttc
    21661 tgccgaacgg ggccgaagaa ggccttgttg ttctggaacg attccggaac cttgcctgcg
    21721 gtgtcggtca cggcaaggag gcaggacccc ccggcgctct tggcgagggc ctccgtgaac
    21781 acgatctgct cgggcccctt ggcaaggagg ctcttgaccg aaggcttcga aggatcgttg
    21841 aggggatgac caccgtcgtc tgccgggtcc ccctccagtt caagttctcc ggcggccctg
    21901 gatgcggtgc gggatgaacc ggaagcaaag gagctctctg cctgccggga agattttgag
    21961 acatcggcga ttcggccggt tcccgggaag ggaccccgca tccggtgccc ggtgctgacg
    22021 cacaggtgtc cggggcaagt cgggcagggt cgtaaagaag tcggtgaagg ggtgggaggc
    22081 ccggaaagga atggggctgc ttgccgtatt cgagaattcc gaccgccgag cctgtcggtc
    22141 cgccttgttt caaagagggg tatgctccgg tccatggacg gtaatctcca cgtatccctc
    22201 ccccctgagg agagtccact atgaattggc gatccatggg tgtcaaatga tttctatgct
    22261 cgcgcggcat caacaggaag gcgacagaaa ctccttgcgg gtccgtgcgg catgaaaatg
    22321 ccggacctgt ttggcggaca aaacgagaaa agccgagtgc aaaaggaatt caaactctct
    22381 tgatgttcgg aggcgaatcc gggagaatgc ccgaacggta tccgaaactt cctcagggaa
    22441 cggcgatctg gggaatcgat gacgcccgtc gggaagatcc cttgccctcg ggtgtcccac
    22501 ggggcgaacg tcccgtagtg gaagaatatg tcgatgtttc catgacttct cagcccagga
    22561 ggaccgacaa tggtatccgc gtggcacctg tgggtgatcg tgggaattct gctcttcatt
    22621 gccgaaatct ttacacccgg gtttgttttg gcatccttcg gcatcgggtg cctcttttcg
    22681 gccctggcgg cagggattgg gttttcgctc aagatgcaga tcctgggctt catcgtcggg
    22741 accctcgtcg cctactttac cgtgcggccc atcttcaccc ggtactgcta caagtcttcc
    22801 cccgacgtca agaccaacgt ggacgccctc atcgggaaga tcggccgggt gaccgagacc
    22861 atcagcctcg aggtgggcac cggtcgtgtg ctggtgggag gggatgactg gaaggccgtt
    22921 tccgtcgaga acacgatcat cccggaacga tccccggtcg aggtcgtgcg ggtccaggga
    22981 accacggtct acgtaaggcc cgtgtaacca aaaacataga gggggaaaac catgggaatc
    23041 ctcatcattc tgtgcgtgtt cgcactgttt gtcattttct ttgccgtcaa ggggttcatc
    23101 atcatccagc agagcgagac gatggtgatc gagcggttgg gccggtatca ccgcactctt
    23161 ccttcgggga tcaacatcat gtggcccctt ttcgacaagc cgcgccagat cgaatggcgt
    23221 tacgtccaga cggaccccgc cggaaagacc atcgttcgca gggtgaatct cggcaggatc
    23281 gatctccggg agacggtcta cgatttcccg aagcagagcg tcatcaccaa ggacaacgtg
    23341 gtcaccgagc tcaacgccct cctctacttt cagatcaccg acccggtgcg ggccgtctac
    23401 gaaatcgcca atcttcccga tgccatcgag aagctcaccc aaacgaccct tcgaaacgtc
    23461 atcggggaac tcgacctgga cgagaccctt tcctcgaggg ataccatcaa cagcaggctt
    23521 cgagccattc tcgatgatgc tacggagaaa tggggagtga aagtcaatcg ggtcgagctc
    23581 caggacatca gcccgcctcc cgagatccgg gtggccatgg aaaagcagat gagggccgag
    23641 cgggatcggc gggccgccat tctcgaagcc gaagggatga aacaggcgag cattctcgag
    23701 gccgaggggg caaagtccgc ggaaatcaac aaagcggaag gagccaaaca ggcgaggatc
    23761 ctcgtggccg aaggggaggc ccaggcacga atccgggtcg cccaggcgga agctgccgcc
    23821 atcaagctga tctccgaggc cgttgcgggg acccggggcg accccgtcaa ctacctcatc
    23881 gccgtccgct acatcgaggc cctcaaggaa atggtttccg ggcaaaacaa caaggtggtg
    23941 tacctgccct atgaagccac ggccatcctg ggctccgttg ggggcatcaa ggacctgttt
    24001 caggcaatcg tcccggaggg tcccggacct tcccgggggt aagccgcagg gggaccccct
    24061 ttgaccacac accggctttc agggggagtc cccggcccaa ccctcctccg acgacgcccc
    24121 gttacgttaa tggaaatctt cgtcggattg cagactccgg ttacatgaaa caggaacaag
    24181 gtcctccgtc ctccgaatag gaggttcagt cagccgccat acactcggta tttccgattt
    24241 atgagacccg gtcgcaaccc tgccgaaaat ccccgcacgc tcccggtgtt ctcctcgtgt
    24301 ttgcggaagt tctttggggc tttccaacgc ctgagctgcg gcacccttct ccgggtaatt
    24361 tcccccccgg attgagttgc attgtcccgg atcatcggaa gaaggcgaat tgccgtcctt
    24421 tcccgaaggg gattgtcctt cgacgaatcg aagagggtgg gaggtctggt cttgtccccg
    24481 gtggagacca ccgagcgcaa aggcacggaa gaggcgctga aggggagctg gggcccggcc
    24541 ccgtcttgcc ctgcaatctg catccatgga cgcctggtat tgggacctga tcgaggacaa
    24601 atgctatggt gacgatcagg cgtttcgcct gataaggatc gagtcgacgc gatccgctat
    24661 gggtgtcgag cggtttttcg aagccgttca ccccgacaat catgacaaga tcagaaaggc
    24721 tcggaactca agtgtcgaac aggggttgcc ccaccaggtg gagtgcagtt tcctccagcc
    24781 tgacggcagc ttccgttccg tgtcttcccg cgccggcccg gtgcgggacg atcacggtcg
    24841 gccgttgaga ctgaacggca tcctccggga tatatccgag ggaaaatggg ccgaggaaaa
    24901 acgagaggaa tcggaagatc gcctgcactg ggcccggaag gccgaaagct tgggccggat
    24961 ggcgggggcg attgcccaca gtttcaacaa cctgttcagt gtggccatag gaaggttgga
    25021 accggccatg acggacctac ccccggcatc gggggcggcg gaggatgttg ccgaggccat
    25081 gacggctttg gaacgatccg ccaacctatg tcacctgacg ctcggttccc ttgccgatgg
    25141 actgcgggcg gcccgctgaa gtgggcaccg gagtcgaacc cgccgatggc cgttccccac
    25201 ggtgtaccct ccttctgcag acagggagtc gtccctcaga atatcatggc cgaaacggcc
    25261 gttcccccca ccaccaccca caggatgtcc accttgagga ccagggccgc caaggctgcg
    25321 gcggccagga cgacatgggg ccaatcccag tggacgttca ggctgaaccg gacggtgacg
    25381 gtaagaagaa gccccacaaa ggaacagaga atgccgccga tggcccgggt aaaccgcggg
    25441 atggagcgca gacggtcgaa ccacggtgcc atgcccacca gcatgaggaa ggaaggaagg
    25501 aaaatggccg cggaggcgag gattgccccg gaaaggccat ggaccaggta gcccacgaag
    25561 gttgccgtga tgacaatggg gccgggggtg aactgtccga gaacgattcc gtcgagaaag
    25621 gtcgaactgt tcatccagcc gcgcgcttcg acgaattcgt gaaacatgag gggtacggac
    25681 acaaatcctc ccccgaaggc catcaggtcg atcttgccca tgaggagcgc aaggtcgaag
    25741 aggtcccttc gtagagcata caatacggcg agggccgcgg cactcaaaag aaccagcagc
    25801 agcaaaggcc gggtggtgtg ggccgggttg ccggaagggg aattgccgga ggcaacgggc
    25861 tgcctgccgt cgaaaagcat tcccaaaaga gccgccagca ggataatgag aaagggattt
    25921 gccccgatgc cgaaaagggc tgccgccgcc agggccacgg ccccgttcct ccagttcttg
    25981 agggaggttc gtccgaaaga cagggtggcg ttggcagtca cggccacgat gactgtccga
    26041 aggccgctga aaaccgagat cacggcgggg agttcatggg tgtgagaata ggcaacagag
    26101 agggccagca tgagaagaaa agccggaaga ccaaaggcaa gaaagctcgt catggctccc
    26161 cgcacgcctc gcacgctgag gcccacgtag gcggccatct gcatggccgt ggccccggga
    26221 attgtctggc aaagggcaac cccggcctgg aagagctctc cgtccatcca ccctttccgg
    26281 ccgactgcca tcttgcgaat gtaggcgatc atggcgggac ctccgaaggc cgtagccccg
    26341 agccgaagga aagagccgaa caattgaggc agggtagggg tggagttcga gccggacatg
    26401 ggttcctttt gccgggtttc ccggttcctc tccttccatt tctggaatcg caatggaaat
    26461 acgtcatttc cgcagaaagc gcaaccgtca aacgtctccg gccgctccgc tccccgtgcc
    26521 ccggacaaag gacgacccgc cttcagtatt cgggcgtctt tgggaaaagc cgggctttga
    26581 caatgccctg atgggcctgc tgaaagaccc ctttgaaacg agccgtttgc ctcaagaggt
    26641 tcgccgtccg caccccgtgc cgggaacctg cggcacaatg ttccggacgg cagcggtact
    26701 tgggccaatc ccgcaccccc gcgggccctg tggtcgggag atccaaccgg aattgcttga
    26761 aacttcaacc ggaggccaat ccggtttgga ggcatgattt cttgcatggg agaaatgacg
    26821 gatgctggtt gtcatgaccg acaccgggtc aatgcaaggc atcaagccga tgaaatcttg
    26881 cagatccagg ccgtcgatcc ttggcagcac gaaggacccg acgtttgccg ttgggcattt
    26941 tttgttgtca aatgtttttg gacggctgta tattcgactg cggcgggctg tgctttgggg
    27001 ggaggggcga agcagaccgc aattgccgca ggaacaaggc gcatgcgagt ttttcttacc
    27061 agccccagca agaggggacg atctctgctc cgggaagggg cggcatcatg aatgtccgtt
    27121 cagtatgcgg atttttcata cgttcttgtc gaggcgggtc gttttctatt cccttccgag
    27181 aagaaacgtc gccatgcccc atgagttgtg aaatcgagtt gccccggggc gtcgatgagg
    27241 ttccaccgaa cgaccacaga gccactgttt gtcgggcacg gcaggacaaa ccacggagtt
    27301 cggaacagtc aggcacaatg ccttgctcgc tgtgaaccgt tgaagcggct cttcagccga
    27361 tactgcccgg tccctgaagc ctttgattct gtctatggga ggttggctgc cggatgatga
    27421 atgcgaacat ccggcataga cccggtctga ttctgagaag ccacggccat ggggcatcca
    27481 atcccgaaat ggacttgatg atcctatctg tttgccggat tgagccattc ggctgccacg
    27541 aatccgtctc ctcattcctg cgaacgtagg cccttcattg gacggggcgc aagccgttgc
    27601 agggatgatt cccgcgcagg cgcaaatcca cgaggagcgc gcataccctc cggatggccg
    27661 ccgtcacggg catgacgggc tgacgccctt gcattcttca acccaggtcc ttgtcaaaga
    27721 atttatcgag gccggccgaa taatgcctgt gcttgcagtt cggtgggccc cgcacccgct
    27781 tcccggttca agatggactc acaatgcccg aggaggccgc tgggacaaat cgggtccacg
    27841 gattcgggat acctgccgga agccgctttc cggctgtgaa acaggagctg acgggtcaga
    27901 gggcagaccg gcaccattca ttgattccat attaggaggg actagatgaa ccgaaaggag
    27961 tccagatttt caggcgcatt gggtgggacc tgtttgcttg ccatcgtggt ggctttcttg
    28021 acggggggcg ctcagttggc tgctgttggc aaagtcgatg cccagtcaaa gaatcaaccg
    28081 gcaatccggg aactgcgggc agccgcgaaa ggaaagatcg caccggatct actggagagt
    28141 ttccagcgtg cccccggtca acccaggcgc tttctggttt atttgaagga ccaggcggac
    28201 ctcaagggcg ctcagggctt gaacgattgg gaggcgaaag gaaatttcgt ctatgacgcc
    28261 ctcaaacaga aggcccggac cagccagcag aacctcttgg gctaccttga gactcaggct
    28321 gccttggggc gtgtagtcgc attcaagtcc tttttcatcg tcaatgctat tcgggtggaa
    28381 gcgggggaag aggtcctgat ggagttggcg gagcgtgagg atgttgatca catcgaagcg
    28441 atcaaggcca ttccggtacc gaagcccgtg gttcaacctg agaggcgtac cgagggaatt
    28501 gaatggggtg tcgacaagat tcgtgctcct gaattgtgga atgagggagt caacggcagc
    28561 ggggtcgtgg tggccaatat cgacacgggt gttcagtaca gtcacggtgc tctggtgcag
    28621 caataccgag gcactgcgac cggcagccac gactacaact ggtttgaccc caccgggacc
    28681 ttcccgaatt tcccggggga caacaacggt cacgggaccc ataccatggg aacgatggtc
    28741 ggcaccgacg ggggttctaa ccaggtcggc gtcgcacctt cggccacgtg gatttcgtgc
    28801 aaaggatgcg agagcaactc ctgttacgat gaccaccttt tggcctgtgc tgagtgggtc
    28861 ctcgccccta cgaacctgtc cggaaccggt gctgatccca cgaagcgacc ccaagtggtg
    28921 aacaactcct ggggtggcgg ggcgtcctgc gacgagtggt atttggcttc ggtccgggcg
    28981 tggcgcgcgg cgggaatctt tccggccttt tcggccggca acagtggccc ggatagcgga
    29041 acggtcggtg cgcccggcaa ctacaaggag agttttgcga ccggggcaac ggattccggc
    29101 gacgttatcg ccggttttag cagccgcggt ccgtccgcct gtcccaactc gggaataaag
    29161 cccaacgttt cggcccccgg ggtcaatatc agatcgacaa ctcccggggg ctacgagtcc
    29221 tgggacggca catccatggc aagcccccat accgcgggct gcgtcgcctt gcttctttcc
    29281 gctcttcctt cgacgagcat cgaagggctg gagagcatgc tcagcaccaa tgccgtggac
    29341 cttggctccc cagggcccga ctactcttac ggcaacggcc gcatcgactg ctatgcggcc
    29401 cttcacggga caaagaaggg ccggctgatt gcagaaccca cttcctttga agtgacgatc
    29461 accaagggtg ccgtgaccac tcggcagctg tcgcttcaaa acatcggcga ggctgctgcg
    29521 agcttcgaaa tcgctgaagt tgatctgggt accgaaaatg cactcggcaa gaggcgagcg
    29581 gacggcgcat cgaagaagca aggccttcca acgggccggt taggaaagcg cgccggtgca
    29641 ccggagcttt ccagacagga tttgccgagc gcacagtcac tgggacggag ggtcccggta
    29701 ccggctgccg cattgggagg tgggaccgtc attcgatcct gggacccggg gatatctgcg
    29761 ccctggggaa ttgcctacga cagcaagaac gacaccgtct gggtatcgag cccggtttcc
    29821 tcctgggaag gggacggcaa tatttatgag tacggcacgg acggggaacc aaccgggcgt
    29881 tcctggccct attcatggaa tccgccatac ggtcccgccg atgcagtctt caatctcaac
    29941 accggcacgc tctgggtcat gaatgtcgac agcagcggtg ggaaaaactg cgtccatgaa
    30001 atcgatcctg gattgggcta cacgggcacc aggatctgcc ccgggggcac gggattcagt
    30061 acgagtcagc gcggcttggc ctacgatccc gataccgact cctactacgc gggatcatgg
    30121 aacgatggaa tgatccacca ttttgcctcc aacggcacaa tgctcgacga ggaatacgtg
    30181 gggcttgaca ttgcaggtct cgcttacaac cccaagacca aacatctgtt cgccgtggtg
    30241 agcgctgatc ccggtccggt gtatgtcctc gacgcggcca accaatactc ggttctgggt
    30301 tcgtttgaga ttcccggttt ttccggatat agcggagcgg gactggagat cgactgcgat
    30361 gggaatctct gggcagtgga tcagaacacc cggatggtct accaggtgga atccggagaa
    30421 gagggggccg gatgcaagtc gtccgatgtt ccatggctca gtgaagcacc cacctcgggg
    30481 tcggtctcga tcgactcctc cgtcaacgtg gagctgacct tcgatgcgac gactctggag
    30541 cctgcaacat acaaggcacg gcttgtgatc tcaacgaaca ccgggcagga cccgattgtg
    30601 gtcccggtga ccctcaccgt gcaggaagag gtggatatat tcaccatcac ggcaacagtc
    30661 aaggacggca agggaggcag tatcagtccg tccggtgagg ttcaggtccc ctacggttcc
    30721 gattctacct tcaccattaa gcccgataag gccaacggat ataaaacggt ggatgtgatt
    30781 gtggacggcg tctctgttgg ggcgagggca acctacacct tctcggaggt ttcggccaac
    30841 cacacgatta cggctgtttt cggcaagatc gtagtgactg ccccgaatgg gggagaaaaa
    30901 tggaaaagcg gcagtaacca cgccatcacc tgggatgctg acggagacat cggcgaggag
    30961 gccgtgatta cccttctgac cagacagggc gtcaagcact ccgtcatcgc caaaagcgca
    31021 agaatagcgc tcaaaaaata caagtggaag atcccgacaa agatcccggc aggtgcctat
    31081 aagattcgca tctccagcaa ggcacacccc aaggtgaagg acacgagcaa caaagtattc
    31141 aaaatcattc ggtagcgggg atggagattg cccggggcac tcccgcatgg atccttacgt
    31201 acggcctcct cccgccgtgg aggtctctct cattcgaccg actcaccctc ttctctgcgc
    31261 gccttgcccc gggcgaaatc ccccggcttt cgcccctccc ttttggggag ggggaaggga
    31321 ttgcgccggg aaagcctggg gaaaaggagc tcctggcgtg ggggggtcgt tcctcaggcg
    31381 gggactccgg cttcttctac cagggagtag aattcctcgg cgtccatggt ttccacttgg
    31441 aggaggcggc tgccgacctt ctcgagaagg gcccggttgc gggtgaggag ttccctcacg
    31501 tgcagggccc gctcttccag gagggttttc acttcctcgt cgagcttggc cgccgtggcc
    31561 tcgctgtatt ccttccccat gaggggcgcc tgctccgggc tcaggaacat ggggcttttc
    31621 tgccggggat aggtgctcag gcccagggtt tctcccatgc cgtactcggc caccatggcc
    31681 cgggccacgt cggttgccct ctgcaaatcg ttgtgggctc ccgtggaagc gtccccgaac
    31741 accagggttt ccgccgttct tcctcccagg agcacgtcga ttttccccaa cagttcctgt
    31801 cgcgtcatga ggtagcggtc ttcggtggga agctgttggg tatagcccag ggcgccgata
    31861 ccccggggga tgatggagat cttgtggacc ttgtccgcac cgggagtgaa ggccgccacg
    31921 agggcgtgcc ccgtttcgtg gtaggccacg atttcctttt cccgggggtt gatcacccga
    31981 tttttcttct cgagcccggc gatgatccga tccacggctt cgtccagatc ttccatttcc
    32041 accgaatccc ggttctttcg ggcggccagc agagcggctt cgttgatgac gttggccagg
    32101 tcggcccctg aaaaaccggg agttttttgg gcaatgacct tgaggtccac ccccggggag
    32161 agcctgacct tggcggcatg gattcggaga atggcttccc ggccgttgac gtcgggccgg
    32221 tccaccagga cctggcgatc aaagcgcccg gcccgcagaa gggccgggtc aagggtttcc
    32281 gggcggttgg ttgcggccag gatgaccact cccacccggg ggtcgaaccc gtccatctcc
    32341 accagcaact ggttgagggt ttgttctcgt tcgtcgtggc cccccaccac gaatcccccc
    32401 cgggctttgc cgatggcgtc cagttcatcg atgaagatga tgcagggggc cttgtcccgg
    32461 gcctgctgaa agagctcccg aacccgagcc gccccgactc ccacgaacat ttccacgaac
    32521 tcggacccgc tgatgctaaa gaagggcacc ccggcttccc cggccaccgc tcgggccagg
    32581 agggtcttgc ccgtccccgg gggtcccacc aggagaacgc ctttgggcat gcggccgccc
    32641 aactgctggt atctgccggg ttccttgagg tagtccacga tctcccgaag ctcctccttg
    32701 gcctcgtcgg ctccggccac gtctccgaag gtggtttcca cttccttttc ggcatatagc
    32761 ttggccttgt ttctcccgaa ggacatcatc ccggcccccg gattcagtcg tttcatgaga
    32821 aggtaccaga tcccgaagaa gacgagagcg ggcagggtcc aggagagcag gtcccggaga
    32881 aaggtggact cgggctgtcc ccgaaaaatc acgttgtgct tggatagctc ggtggagaga
    32941 tcggggtcca cgcgaatggt gatgaaagcg acctctttgg gctgctggtt ctcggtgacc
    33001 ctcatttttc cggagatgcg ctcctgggtg atgaccacct cgatgatgtc cccggcctga
    33061 agagacttga ggaactcact gtagggcagg gcacggggcc gatattgagc ggcgatgaaa
    33121 tcctggatga tgagcacgcc ccagacggcg gcaatgacat accacaggga gaattttgta
    33181 tgcctttcca tgattgcgtc ttcccgtgct caggaggacc gggaggcgag gaccgaagtc
    33241 ccgtgatcgt ccttccggcg aatcccggac cgggtccggg gcatcgccgg aatccgtggg
    33301 ggcaagagac ctgggctacc ggtgtttctc ggggcggtcc gggtggaggc cgcctctgct
    33361 cagtccccta agctttctgg aggttcgctg ctgcaaattc ccaattgagg acctggtcga
    33421 tgagggtttt cacatagtcc gcacgccggt tctggtagtc caggtagtag gcgtgttccc
    33481 agacatcgat ggtcaacagg gccctttgcc cctggacaac agggttgtcg gcgttggagg
    33541 tgctcgaaac cttgagcttc ttcccatcgg ccgtcagcca ggcccatccg cttccgaacc
    33601 ggcctacggc gacctggagg aattcctgtt tgaaattgtc gaggctgccg aaagaggctt
    33661 cgatttgctt gaggatttcc ccttcgggct tgctccctcc gggcttgagg ctgttccaga
    33721 agaactcatg gttccaggcc tgggcggcat tgttgaaaac ggcagccttg tccttggagc
    33781 cgtaggtctt tttcatgatc tccttgaacg agagtcccgc caggccggta tccttgacca
    33841 gttcgacggt cttgtccaca taggcccgat ggtgcttgtc gtaatggaaa tccacggttc
    33901 gcttggaaat atagggttcc agggcgtcca atttgtaggg aaggggaggc agggtcaggg
    33961 acgccgtgga atgggaagag gagcagccgg ataggccgac cagtccggac ccgagaacca
    34021 cgaatcctcc tgccatcgtc cggagaaaac tcctgcgatc aaaactgggc tgagtttttg
    34081 tttccgtcat agctgcctcc tgtgaaataa gcgttcggtt tctggaattt cgtcgggatc
    34141 gtccccaaaa ctctgctttc ctccagggtt tctgcaagct ggattttgaa tgctcttggt
    34201 cgcccggttt cgggcgaaga aacggtcgcg tcccttcccc ggtgaacgct tgccgagggg
    34261 tcgtagtagg gccgatttcg ggcctgtcgg ccgcttcaaa gtaaatcgtt gcccgaaaaa
    34321 acgcaagaat agtaagagag cacttcgtgc cgtttggaac gaggacgtgg cccggcacct
    34381 cgcgatgccc gaaaggcatt tcaaggaaag gccggtgctg tccgtttcgg gtgtcggcga
    34441 ggcggtgccc ccgtgatagg cgtcaaggat cctgtcctgt catggttccc gggcaaggac
    34501 gtcacttccc caaaggaaca agagagaaag ggttcccatg agaaagaaca cggagagaga
    34561 ctcaccgcaa ccgtggctct ggatgacgat tccctccttt gtgagggcga gggctcccgt
    34621 ccaccgactc cccttggcgg gggcggagtg gaaaagtccc cctcgatttc atgcaccggc
    34681 cgagaaagaa cacgtttctc cgccgcagga gggaaacccc ctccatggct gaccggttca
    34741 ccggaccttc gttccaacgg ttttctccga aacgggcggg ccttgcgccc gcccgaaccg
    34801 gtgccgagag ccttggaacc gctcaaaccg ggtataggaa aacggccgtg tggagtgacg
    34861 gaaaccggaa acgagatccc ccgcaccccg accctcctca ccgaagccgg ggagcctcct
    34921 gcccatggga aatgggttgg ggcccctcgc ccccaaccgt cagtctttca ggaacaaggc
    34981 gtggtactcc cggtccgcca ggtggttccg cagggtttgg ctcagcatct cgaagatccc
    35041 gttgatttct tcgtcgctca atcggggcac cagcaccttc aggagttcat cgtccgaaaa
    35101 cttctgcagg tagcaggtca aggacttttc atccacgtcc cgagacatgc cgaaggcgat
    35161 caggccgtca taggtttcca cgaaggtgtg tcgatgcttc gacatgggaa ctagtcctct
    35221 tcaaggtcca tgtagtaatc cagcccgaaa tgggtgatct gttcttctcc cttgagatac
    35281 cgcagggtgt tcttgagctt catgagctga atgaaaaggt cgtgctcggg atacaggccc
    35341 tctttgtgca tggggctctt gaagtagaaa gagagccatt cctggatgcc tcccatcccg
    35401 atgcgctggg cgagttccgt gaagagcacc aggtccagga taaggggagc cgccaggatg
    35461 ctgtccctgc actggaaatc caccttgatc tgcatcgggt accccagcca gccgaagatg
    35521 tcgatgcagt cccagccctc cttgttgtcg ccccggggag gataatagtt gatggtgacc
    35581 ttatggtagt agttcttgta caggttggga taaagggaag gctggagaat gtgctcgagc
    35641 accgacagtt tgctttcttc cttggtcctg aaggaatcct tgtcatcgag caccaggccg
    35701 tcccgattgc cgagaatatt cgtggagtac cagccctcga ggcccagcat tcgggctttc
    35761 attcccggag cgaggatggt cttcatcagg gtctggcccg ttttgaagtc ttttcccgcg
    35821 atggggatgt ttttctcccg ggccatgagg accatggcgg gaatatccac ggtgagattg
    35881 ggggccccgt tgatgaaggg aatatcgctc tcgagagcgg ccaaggcata gagcatgctc
    35941 gggctgattc gggactcgtt gcggtccagg gcctcgagga atgactcggg ggaccggtga
    36001 aagtcggcgg gttcgaggaa ggcctcggtg ctgccgcacc agatcatcac gcaacgatcc
    36061 gccccgtgaa ccctcttgaa ctcccgaatg tcctctttca gggcttccac gtgatcccga
    36121 aggttggacc ctttcttcac gaaggtcccg tccaggtttc gcacgaatcg ccggtcgaag
    36181 acccctcgca tgggagtgat cccggagaga aatcccttga tgggttccag gtgatcgcga
    36241 ttcagcacgc gggcatacat ggcggcttcg tagccgttgg cttcataaat gtcccagccg
    36301 ccgaagacca gatcgtccag gcccgccagg gggataaaat ccttaatctg aggagtccgg
    36361 tgctcgaatc gcttgcccaa ccggatggtg cccaattgtg tgagggaccc gacgggtgcc
    36421 gcgagtccct ttcgaaccag ttccaccccg gcgacaaagg ttgtgctcac tgcgccaccc
    36481 aagccgggaa tcagcacccc caatttccca ctcgcaggag ggatctggtc cttccgcttc
    36541 attttcctgt ccttcaacgc ttgtcctcca ttctagaatg acaattcccg gagccgctct
    36601 ccagctccag cagattcctt ttcacctcga tgccaccgga aaatccgccc agctttcccc
    36661 ccgccccgac cacccgatgg catggaacga acagggccac ggggttccgt ccgcaggcct
    36721 gtcccactgc ccgggaagcc cggggagacc ccatctgcgc ggcgacctcg aggtaggtac
    36781 gggtttgacc ccggggaatc cttcccaggc actgccagac cctccgttgg aagtccgtcc
    36841 ccgacgagag atccagggga aactcctcga gggggttgcc ctcctgcagg tagccgcaaa
    36901 cgctttcccg ggctcgacaa agcaaagact cgacggcgga agaaccccga tcctcccccc
    36961 caatggaggg cagatggaga atcttttgaa tttcgctctt ccggggccca accgctccga
    37021 agaaatgcag gcggcaaatg ccccgttcgt tggcggccag gaggatccgg ccgcaggggg
    37081 aagggaaggt cgtataggaa atcggtcctt gctcttcctc tctaatcatc gatgaaatcc
    37141 gtgatgtaag ggttggtttc ccgttcttcc cggacggaag agtagggccg atccccgtag
    37201 tcatgtcccg gccagacgac ggtctggggg ggcagggcca ggaccttgtt cttcaaagat
    37261 tggatgagct gatcgaaaga cgctccggga aggtcggtgc ggccgacggc ccccacgaaa
    37321 agggtgtctc cggtaaacag atggccgtcg atgagaatgc agcagccgcc cggcgtgtgc
    37381 ccgggcgtgt ggagaaactt catggtgatg tccccgaagg acagctccgt tccgtccccg
    37441 acccgcacat cggccggggg agaggggaca aaccccatca ttcctgcaaa ggcctgggat
    37501 tcgggtctct gaaaaaacgt gtcgtcggtg gcgtgcatga cgatgggcgc ccccgtccgt
    37561 ttcttgagct cctcgttgcc gcaggtgtgg tccgcatgcc cgtgggtatt gacgatgtac
    37621 cggagcttca ccccccgctc ttcgaggagc ttcatcaggc gatccgtgtc tcctgcggga
    37681 tcgatgagaa tcccgtcccc ggaggcctca tcgtatacga gatagcagaa aacttccatg
    37741 acacccacga tggtttgtat aatcttcatg gaattctccc agagtggatt ttctttccga
    37801 cttcaggcgc gcaggttttc aacatggacc aactccggga aaaagtcaaa gggaagggac
    37861 gccgggggat ggaggtggat gggcccatgt cggactcgag ggagctgttc gtggttgaat
    37921 atcggggagg ccgttgcccc tcgagttttc ccggcgtcga gggcctgttg ggcgtgtggc
    37981 ccgagcctcc ctacttttac tgcttttttg gtcgggaggt gtcacgggaa gagatcgctg
    38041 gctttggccg ggaatacggc tggactttcc gggacacgta tcgcctggcc tacgatcagt
    38101 ggcaggatgt ggcgcccgca accctgcggg tggggccctt tctgctgcat actccgggaa
    38161 cgccgcctcc tcacccggag agggccgaag ggatggtcct gcacctgaac cccggcattg
    38221 ttttcggctc cggggtccat ccctcgaccc ggggctgtct gctggtcata ggggagctct
    38281 tcgcatcctt tcccatcgag acggtcacgg atttggggac cgggacgggc attttggccc
    38341 tggcctgcgc ccggctgggg tgccgccggg tcacggcgat ggactgcaac cccctggcgg
    38401 cccgagaggc cctggccaat gtgcgtctca accggctcac ctccgtcatc cacgtggtca
    38461 ccgccgacga tccggcaact ctcaaccggg gaggcgacct cttgctcatg aatatcgagt
    38521 gggcggctct gcgcaaggtc ctcgagggta ctgcctggct ccgttacggg aaggtggttc
    38581 tcagcggttt tctggagacc cagtgggggc aggtccgcga catgcttccc gcccactatt
    38641 ccatcttctg ccggaggagc gtggacggtt gggggaccgt ggcggtggca aaccctgcgt
    38701 cggggaatac aaatcccggt ggttcggggg cgagggaacc cccgtgggag tcttcccggc
    38761 agcgctttgg acggggggcc ggggggagac cggagaaaac cggggatccg agcaataaaa
    38821 acccggggca ggtcgaaccg gccccgggtt tcgttcccac cgcaaacggc ttcgtcaaaa
    38881 gcccacgtcg ggacaggctt tcagggccgc ttggatctgt tcttcggaga atacctcgtc
    38941 cttgagctct ccccgcaggt aattgtcgta agcggcgagg tccaggagcc cgtggccgga
    39001 atacaggaag acgatgacgg ttccgggagc cgcttgccgg gcttcgtcaa tggccgctcg
    39061 aatggcgtgg gacgtctcg
//
LOCUS       DAOOVC010000022        38917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_670_length_38917_cov_6.074983, whole genome shotgun sequence.
ACCESSION   DAOOVC010000022 DAOOVC010000000
VERSION     DAOOVC010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 38917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38917
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_38917_cov_6.074983"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..2113
                     /gene="kaiC"
                     /locus_tag="PLM79_04895"
     CDS             461..2113
                     /gene="kaiC"
                     /locus_tag="PLM79_04895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HOV85677.1"
                     /translation="MPHRNRSKETFSASPLAKAPTHIKGLDTVLEGGLPRGRTTVVNG
                     GPGSGKTLLALEFLYRGALAGEPGVFIGFEEPLDQLRVNAGTLGWDLAALERDRRLCL
                     VEGRLKPDTLVSGEFSLKGLLAGASGVGREIGAGRVVIDALEVVLRLYDSLRQVRNDM
                     HLLNDWLQAEGLSAILTVRPPVFGSVPSFHDFFDSMADCVIYLDARVDNQLSRRRIRV
                     VKYRGSGFGSNEYPYVITDSGLHIEPISTVGLRHKPLGERVSTGLARLDDFLGGGYRR
                     GACILVAGSPGTGKTLLAGTFSLAACGRGEKVLYVGYEESQEAVIGNMGHAGVFLKPC
                     VDTGLLLFHTVMPEAMGPEEHLIRVMNLIEAHNPRHLVVDAISACGRMGGKQAAFDFL
                     VRLLNRCKERNITVLLVNQLSGTTTHMEVSGNDISSIIDTVLFMSYVEGAGEINRVFQ
                     VLKSRGSGHSNQKQEYIITDEGIRILEPYTGEGEVLTGSARRLQEARDAAEAQRLAVA
                     MKAKEIELEQLRIEQRQITERKRYQAQVRGAAPGSADGEDRS"
     gene            2110..2436
                     /locus_tag="PLM79_04900"
     CDS             2110..2436
                     /locus_tag="PLM79_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HOV85678.1"
                     /translation="MTKDGSPSGTASQRHGLYRFRLFVAGNEVNSQRAKETLFHLAGT
                     YLADRHEIVVVDVMQDYQAALDSRIVVVPTLLVESPFERVIVGSLSDEKAVLDALGLA
                     PERRPQ"
     gene            2433..4949
                     /locus_tag="PLM79_04905"
     CDS             2433..4949
                     /locus_tag="PLM79_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85679.1"
                     /translation="MSGRKPSYEELETRLERAEAVLDSLRRGEFDLIIGLREPIVVQF
                     KSLAEEKERLAREWQTTFDAVGDAIWLLDGDQRILRTNRAAEKFFQRPADALIGRHCW
                     EISHGTPRPIPECPLLRLRRSLRREQTEMPVGDRLLYVTVDPILDAGGKLTGSVHVVS
                     DITERKRAEERIRRSEETYRNLFCNAQVGLFRTRISDGKILESNEQLARMFGYDTREE
                     FIAEYATLQNYVDAGTREKMLERIRRAGSIEDFEARFYRKDRSIFWARYSARIDPEQG
                     WIEGVAEDITEQKLTEETLKIQLRILSSFHTIPDEEIFDEVLKVILDVMHSPSGLFGY
                     LDGALVVPTMTYQMWDSCRIADKKTVFPPAQWGDSSWGRAIREKKANYSNDPSTKTPA
                     GHIPVARHINMPVLFRDEVIGLLTLANSETEYTEADVRRLEAIADQVAPLLSARLQRV
                     RAEKEKDRLRTELAQAQKLESVGRLAGGVAHDFNNMLGIILGYGDILMQKLHPGDPLR
                     DDVKKIVEAGKRSASLTRRLLAFSRKQTLQPEVLNLNSVLRNLEKMLRRLIGEDIDLK
                     LTLADDLAHVLIDPGQFDQVIMNLAVNARDAMPTGGKLTIETANVAIEEQIQTAVPPG
                     QYVMISVTDSGCGMDETVLPHIFEPFFTTKEKEKGTGLGLSTVYGIVKQSGGHIRAYS
                     GPGQGSTFKIFLPPTHVKPQVEKISLQERRQIGGGKRVLVVEDEESMRELLRTILSRL
                     GFEVFVAANGGEALLRVEEQGLEPDLVLTDVVMPGMSGAVLAERLRRSQPDLKVLFMS
                     GYTDDAIVHHRMSDHGTPFIQKPFSIDDIAEKIRRVLQNG"
     gene            complement(5459..6805)
                     /gene="rsmB"
                     /locus_tag="PLM79_04910"
     CDS             complement(5459..6805)
                     /gene="rsmB"
                     /locus_tag="PLM79_04910"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOV85680.1"
                     /translation="MRVRILAVQILLHLDQKVSHPDRLLRTTLARHPGMDERDRALLS
                     EIVYGVLRWQGRLDWHIDRLSTLKPRKISPAVRVLLRCGLYQVLMLDRIPPHAAVHQT
                     VEIAKSSQPPYVVRFLNGVLREASRRGDHWEWPSEETDPVEHLSVTTSHPRWFVEQCV
                     RSWGLEETREICRANNRVAPLSLRINPLKADRPRVMEWLSTNGIAAEESPYLPGAVRI
                     AGIRQDPARLPVCENGWVQVQDEASQLVSHLVAPRPGERILDLCSGFGGKSTHLGILM
                     EDRGEILAVDQSAWKLEDLRENASRQGLHILRPVRGDVLDLRPEDLGVFDRVLLDAPC
                     SGWGAVRRKPDIKWRRHPKDSYRFSRQQKALLDHAAKFVRPGGSLVYATCTLFPEENE
                     EVALAFGETHGEYRVLPAEEVLPESCRSMSGGPFFRTWPHRHGVDGFFAARWTRRD"
     gene            complement(6805..7557)
                     /locus_tag="PLM79_04915"
     CDS             complement(6805..7557)
                     /locus_tag="PLM79_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOV85681.1"
                     /translation="MTLVGLFGLTAVLILALLGLSYFIPYYGFGRIHQSLPILMALLI
                     GGVGLLVLVAFVLLIVVTLLGRDVPFSQKLRSLVIKLLLPVLIKVGRLVGLRKEEVQH
                     AFVALNNELVMAQCRNGHPPQKILLLMPHCLQNHDCPVKITYRVENCKRCGKCPIKDL
                     LELASRYGVDLAVATGGTFARRIVIETRPDLIIAVACERDLTSGIQDTTPLPVYGIFN
                     ERPFGPCFDTRVGLEHVESILREVRSLKPEGS"
     gene            complement(7721..8668)
                     /gene="fmt"
                     /locus_tag="PLM79_04920"
     CDS             complement(7721..8668)
                     /gene="fmt"
                     /locus_tag="PLM79_04920"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOV85682.1"
                     /translation="MNRELPKLVFFGTPDFAVPTLRRLGAEKGTVLLVVSQPDRPKGR
                     GRKTSPSPVKAFAEGAGIPVFQPARVRDPEAVATIASTGAECAVVVAYGQILPRELLD
                     LFPLGVLNIHGSVLPAYRGAAPIQRAILAGDRTTGVSIMLLDAGMDTGPVLSRETISI
                     EESDTFGSLHDRLAEVGADLLVKTLPDWKRGRIVARPQDEGLATYAPPIGKEELKVDW
                     TRPGLEVVNRIRAFDPWPGAHCRWEDRRVKCFKASLAALSSPGTPGEVLGETGAGLLV
                     VAGDGRTVCIRELQLEGQRRLAAVEFLRGHFIPGGTILR"
     gene            complement(8665..9168)
                     /gene="def"
                     /locus_tag="PLM79_04925"
     CDS             complement(8665..9168)
                     /gene="def"
                     /locus_tag="PLM79_04925"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOV85683.1"
                     /translation="MQIRKYPDPILRKKAVEVGRMDREVRRLIDDMTETMYGAPGIGL
                     AANQVGRPLRVLVIDLQKPENNDGLIVLVNPQLTESQGEIVYEEGCLSVPEYFAAVKR
                     YGEITVRGFDRDMKPVEIRASGLLAVVLQHEMDHLDGKLFIDRLGPITRDIFKRKWKK
                     KQKEGQA"
     gene            complement(9342..11063)
                     /locus_tag="PLM79_04930"
     CDS             complement(9342..11063)
                     /locus_tag="PLM79_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOV85684.1"
                     /translation="MNQSSCGMESPSAPKNAEAPAVAVIGAGYWGRNLVRNYAQLGAL
                     RLICDKNEDLLAHLQESYPRVELCLAFNEALSRNDIDAVVVATPAETHFALVREALLA
                     GKHAFVEKPLVLSEPEAEELIGLARRQKRTLMVGHLLQYHPAFVRLRELADSGELGRI
                     NYIYSHRLNLGKIRREENILWSFAPHDISMILSLAKEQPETVLATGGNYLHRKIADVT
                     TTHLEFPSGLKAHIFVSWLHPFKEQKLVVVGDRKMAVFDDTKPWSDKLLLYAHEIRWE
                     KSVPVPAKADPERVELPESEPLKLECAHFLQCVRTGARPTTDGEEGLSVLRVLNASQR
                     SLNRYGECVPIAAVKSMAGGEPSSYAPLEAAETRVPAGLPLEDPFIPEGKPLPEAPAV
                     EPDFFAHPSAVLDPGVRIGTGTKIWHFSHILSGSIVGKNCSIGQNVVVGPEVSIGDGC
                     KIQNNVSVYKGVTLEDEVFCGPSMVFTNVYNPRAHIRRMDELRPTRVKKGATLGANCT
                     IVCGTTIGRYAFIGAGAVVVRDVPDHALMVGNPARQVGWMCRCGTRLDEDHACPACRQ
                     KIDGLGA"
     gene            11317..12279
                     /locus_tag="PLM79_04935"
     CDS             11317..12279
                     /locus_tag="PLM79_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV85685.1"
                     /translation="MREGKRIGVVVPAYNEEALIGRVIETMPAFVDGIYVVDDCSRDS
                     TCEKVLSSKAGISENQRLHLIRHEANQGVGAAITTGYRRAVEDGMEVIAVMAGDAQMD
                     PDELDKIVDPVIRGEADYVKGNRLFTGQAWHLIPKYRYMGNAFLSLLTKVASGYWHVA
                     DSQTGYTAISAQTLRSIPLDLLYPRYGYPNHLLVMLNIYEQRVKDVPVRPIYNIGERS
                     GIRLRKVIPRLSWLLLKCFLWRMKEKYIIRDFHPLVFFYLMGFSLLPVGMVFGTYLFI
                     HRLLDGPVAATSALFSVFLAVTGLQALFFGMWFDMEYNKELKVR"
     gene            12390..13406
                     /locus_tag="PLM79_04940"
     CDS             12390..13406
                     /locus_tag="PLM79_04940"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOV85686.1"
                     /translation="MDLKNKRFLCIGGAGFIGSHTVDALLKEDVAEVRIFDNFTRGSR
                     ENLAEALEDPRVSVFPLGGDILHTDILDAAMEGMDGVFHFAALWLLHCYDFPESAFEV
                     NIRGTFNVLQSCIRKGVKRLVFSSSASVYGDAVREPMNEDHVYNNKTFYGATKIAGEH
                     LCRALYHRYQGTDRHFDYVGLRYMNVYGPRQDYHGTYITVIMKILDRLDQGLPPILYG
                     DGSQSYDFIYVGDCARANVCAMKADSTDSFYNVGTGRKTSLREIAELLLELTGSRQEI
                     QYEEAGLTFVKNRVGCPKKAQSEIFFTADTDLRRGLSQLIQWRAGHKEHVKRLRAGKG
                     SVRP"
     gene            13403..14542
                     /locus_tag="PLM79_04945"
     CDS             13403..14542
                     /locus_tag="PLM79_04945"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOV85687.1"
                     /translation="MKPKNIPIAVPHLEEEEWLALREPLETGWLTQGPKVAAFEKAFA
                     ARHSAKHAVATTSCTTALHLILAALEIGPGDEVIVPAFTWVATANVVLYCGAVPVFAD
                     VDRATYNLDPAEIPGKITPRTRAVIVVHLFGLCADVDAVAKAAPGIPILEDAACAAGS
                     TYRGRSAGCLGSAGAFSFHPRKVITTGEGGMITTNSEELAQKLTVLRNHGAGISEEER
                     HRGPRPYLLPEFSHLGFNYRMTDLQGALGLVQLGRLDGLIAQRRQWAEYYNRALGGIP
                     WLHLPVPPPGYGTNWQSYVCRIDAGAPCSRNEIMEKLQSRGISTRPGTHCVPTLDYYR
                     RRFDLRPEDVPVAWDSEHQTIALPIHSRMSPQDYDRVVEALRELG"
     gene            14548..16485
                     /gene="asnB"
                     /locus_tag="PLM79_04950"
     CDS             14548..16485
                     /gene="asnB"
                     /locus_tag="PLM79_04950"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOV85688.1"
                     /translation="MCGFVGVCNRNGEPIPHVLLKEMTRVIAHRGPDGEGHYLDDSVA
                     LGHRRLAIIDLSPAGHQPMTNEDGNVVLVYNGELYNYQKLRAQLVALGHRFHSQTDSE
                     VVVHAYEQWGPGCLEYFNGMFAFAVWDRTRRRLFLARDRYGIKPLYWYAQNGLFLFAS
                     EVKAILVHPRVSPEVCYPALNEYFTFQNIFSDLTLFEGIRLLPPGSSLTLDVREGTGP
                     VLHKYWDYPFDGGKLNLSPRESADELFRLFIQAVTRQLVSDVPVGAYLSGGMDSGAIT
                     ATAVRHLPRLTTFTGGFDLTSASGLELAFDERPVAEMMANLFKTEHYEVVMHAGDMEW
                     VLPELIWHLEDLRVGQSYPNYYVARLAGKFVKVSLAGTGGDELFGGYPWRYYRCLDNA
                     CREDYFKRYYDFWQRLVLDNEKPRLYNSDTRRGIGDHSTFEVFRRVYDQWTLPLRENG
                     DYVDASLYFELKTFLHGLLVVEDKLSMAHSLETRVPFLDNDLVEFAVRIPAHHKIRDL
                     QCAAPMDENLPGKRKLYDLQTANGKVVLREAMSRIVPREVIQRHKQGFSAPDASWFRG
                     ESIDYINRLLRNPKALIYEFLNPAFVGEVLDQHCSGKVNRRLLIWSLLSFEWWCRCFL
                     HEPYSKWRPHPAGETRRGVRM"
     gene            16482..17054
                     /locus_tag="PLM79_04955"
     CDS             16482..17054
                     /locus_tag="PLM79_04955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOV85689.1"
                     /translation="MTPAARLFESLRHLYEKHRLRMKKRWDRDLPFDELLFDRFQRAR
                     NLGFGEGSSVYHNSYVYGDVKVGRSTWIGPFTLLDGTGGLEIGDWCSISAGVQIYTHD
                     SVKWALSGGKLPYERSPVRVGNRCYIGSKTVIAKGVTIGDQCVVGACSFVNKSLPPNS
                     IAAGIPCKVIGTVRTDDEISFHYHQVPESS"
     gene            17080..18309
                     /locus_tag="PLM79_04960"
     CDS             17080..18309
                     /locus_tag="PLM79_04960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85690.1"
                     /translation="MRICWVTTSFPRFVGDHSATFLLTLAKHLVRKGVDLTIVAPHEG
                     GLPEEETLEGVRVCRFVYWLPRRSQALAYGHGIPDNIRRNPSVGLQVPPFLASAALKV
                     LSMARGADVLHGFWTPSAFLAVPAKKRYGIPLVTTLLGSDIRNGPKTVNRIALHFSDA
                     VVCATEEMAAYLRTYPVKVPVFDVKQTNLVDPDRLRSGDSLEPDLLEWIGKGSALITL
                     VARLVDFKDPVGFVHAMPYILQEHPGTRFLIIGDGPLFEPLETLIRSMNLTSCARLTG
                     HRTDIGSFLERSTVFVANSPVSNCYSCAVLEAMSAGVPCVLTDAGDPRGTFRGYVELA
                     RPRDPRSLAQAVNTLLSDEEQRRHRAATSRQFLLDLGFGSEVVLRQTMEIYEALRRDP
                     KLPDRLSIPRVDLSPGI"
     gene            18329..19327
                     /locus_tag="PLM79_04965"
     CDS             18329..19327
                     /locus_tag="PLM79_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85691.1"
                     /translation="MGTHFKSTWVKGLKRSLLALTLVFVGFALYRSVLNADWSLVEVR
                     PGRLFLGFLLIAVSTVVGSWILGYIYRNLGLQLTPCQALELLTVPALGKYVPGKFLSI
                     AGHAAMALSFGIPLPAGTVAVGVATSIGLAASTLLGLLVFLLSPDSFQGLVRIGWPEW
                     GILLALLFLVLCPGLYRQVLQFFLRIFKHPPLPFHLSFGTLALLLTTNMLQAGLHVTG
                     FAFVVTGMLHLPVHALPTIGGALCLANVVGFLALFAPGGIGVREGILLLLLAPSLGDA
                     PAGFVAVATRLIQVAADLLAASMGATVAGVERRRNERSDPAQAPMQAGRTGKPPRA"
     gene            19959..20399
                     /locus_tag="PLM79_04970"
     CDS             19959..20399
                     /locus_tag="PLM79_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV85692.1"
                     /translation="MFIKMKESKGFTLVELMIVVAIIGILAAVAVPFYQRYVQKSRLT
                     SLAFPAMHSIETNLAGYFAIRNTFAGVSMTAMIQDAEMTCIVKNPTWDQNSRLLTFVI
                     NTQASCPNLAQFAGRTITARASVTGNKIQTWVLGGAIANELGLK"
     gene            20493..22961
                     /locus_tag="PLM79_04975"
     CDS             20493..22961
                     /locus_tag="PLM79_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV85693.1"
                     /translation="MEERNPIFTQNKKLYLAALGVVCLMVFAAYCNTFYAPPALDDFH
                     SFIDEPAVRINSWSIEDLRRLSTTMFGWSRWLPMISFSLDIWVGEGSLFQLHLTNLLI
                     HISTFLAVLFLVFALSRHAKECPSTSESLPDPSTLALWVAGLWALNPVHTSAVTYLVQ
                     RMAAMMALFFVLSVAFYAAGRLLMLRRGGFSAKAGLCFLLSFLTMIMSFFSKQNSVML
                     PFTLVCVETWFFRPQLPGEVWNFLKRRRILSLIALGIILIVLYYKIPPLLVGYSHRHF
                     TLTQRLLTELRVVVWYISVILWPNPGRLSLEHDIPLSTSLIHPPSTLACLLVLVSMIL
                     WAVRKRQRFPLASFGIVWFFLNLAVESSFLPLELVFEHRVYLPSVGLMLSLVVLVYTG
                     VRKAVPEAPRETFLKLGWCLAAILASALTLATFSRNTVWENSVDLARHDAQVNPNCPR
                     AHVNLAVALNRAHRFEEALESAYKALEVTRPNYEAYSVAANALVIAHSQLGNYETGIR
                     EAERILRETPPKADAGAAPLALINIGRAHRKMGNLEQSYQAARRALTTHQRLTPQKIP
                     EFELLVMSLLSDVYVDAREGNIPVDFDEDGEPDSLEMPRIVWMAEQFHKWNDRETART
                     LLTQAAADHPDDLRSAELLSQYRREDEMNRIQEEKKDFTSKYLRWPSDRFRACMAIAF
                     LTEKKKLPSFFAGIGEAALDYALRLRPDSADAHLLKAWFCYNQDKSEEAIGRAQKALA
                     LDRDYARAWLGLGFFLAKAGRTEEALDAFQKCLDLYPGNPDRVAITTIAAELKSKFEG
                     RQAVNDPKRESSARRPSPEGFHPG"
     gene            23187..23834
                     /locus_tag="PLM79_04980"
     CDS             23187..23834
                     /locus_tag="PLM79_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOV85694.1"
                     /translation="MKTTPSLWERGAAIAALCCFSGILFSFAGTAAGTTPPEAAVQTP
                     SEAAGPPEEASPPVPKETKGPKGTEEALQSRVKEALLRESFSYPTGDLLDPFIPFISA
                     VETPPPQIASKEETDTELPPEQQRPLTPLQKMTLAEIEKGLRAITWGDLGRKAIIEDA
                     AGKGYIVGIGTPVGDKNGVVTQIFNDRLVIQQEVWDRQSKKMVAVNSVVRIKKAK"
     gene            complement(23859..24320)
                     /gene="lspA"
                     /locus_tag="PLM79_04985"
     CDS             complement(23859..24320)
                     /gene="lspA"
                     /locus_tag="PLM79_04985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOV85695.1"
                     /translation="MKLLVPVMGGIVLLDQLTKAMVREWMPLHTMREIVPGLFNLVHV
                     RNTGAAFSLLAGAHSSLRLVFLVGLTLLVMGILFYAYRKLSPRDRWVRAAYSLILGGA
                     AGNLVDRVWLGEVVDFLDFYVGTYHWPAFNVADTAISTGAVMVLLSFFRWK"
     gene            complement(24368..27148)
                     /gene="ileS"
                     /locus_tag="PLM79_04990"
     CDS             complement(24368..27148)
                     /gene="ileS"
                     /locus_tag="PLM79_04990"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOV85696.1"
                     /translation="MDYKNTLNLPQTAFPMKANLPKLEPEILRQWETMGLYEKLREQG
                     QGRPPYILHDGPPYANGHIHLGTALNKILKDMIVKARQMSGYDAVYVPGWDCHGLPIE
                     HQVDKELGPRKKSMTQVQIRRECRRYAEKFIDIQREEFKRLGVLGEWNHPYLTMSYDY
                     EAVIARELGRFFVNGSVIRSKKPIYWCALDRTALAEAEVEYHDHVSPSIYVKFPLRAE
                     GRDAFPELAGRAASVLIWTTTPWTIPANLAVTLHPDFEYVAVEVGDEAWILAEGLLES
                     CMKILNVGEYRVLRTFRADELRGLRCRHPFLDRDSVLILGTHVTLDAGTGCVHTAPGH
                     GREDYDMALEYGLEVYSPVDDGGAFTPEVPLFAGQFVFDANPSVVAKLKETGNLLLET
                     RITHSYPCCWRCKKPVIFRATEQWFISMEKNDLRQKALEWIDRVQWVPSWGRERIHNM
                     IANRPDWCISRQRSWGVPIIVFTCEDCGGVLAAPEVFDHVSALFEKEGADCWFERSAE
                     ELLPENAKCTGCGGRRLKKEMDILDVWFDSGVSYAGVLEARPYLRSPADLYLEGSDQH
                     RGWFHSSLLAAVGTRNAAPYKTVLTHGFVVDGQGYKMSKSLGNVIAPEEIIRQYGAEV
                     LRLWVSAEDYRDDIRISPDILKRLSEAYRRIRNTCRFILGNLYDFDRASDALPYEEME
                     ELDQFALHQLQDLVQRVRNAYDRFEFHRVYHSIHNYCVVDLSAFYLDILKDRLYTSAA
                     GSKARRSAQTVLYEILSALLRLMAPILSFTAEEAWRHLPGHSGESVHMEPLPDEKAEY
                     RNEVLNERWQKILALRADVSRALEAARQAKKIGHALDAQVSLRLPASYEEMLRGGEGL
                     MRSVLIVSRVVFAGEDLEDAVEGQEVEGLKIAVKPAPGAKCERCWVHSERVGEFFDHP
                     SLCERCYGVLTG"
     gene            complement(27710..29344)
                     /gene="groL"
                     /locus_tag="PLM79_04995"
     CDS             complement(27710..29344)
                     /gene="groL"
                     /locus_tag="PLM79_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696997.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV85697.1"
                     /translation="MAKQLIYDVKARESLLKGVNTLADAVKVTLGPKGRNVVIDKSFG
                     GPTVTKDGVTVAKEIELEDKFENMGAQMVKEVASKTSDVAGDGTTTATILAQSIYYEG
                     SKLVAAGANPMALKRGIEKTVQVVVDELKKISKPTKDQKEIAQVGTISANNDSTIGNI
                     IAEAMNKVGKEGVITVEEAKSMETTLEVVEGMQFDRGYISPYFVTDPEKMEVLLNEPL
                     ILINEKKISGMKDLLPVLEQIAKMGRPLLIIAEDVEGEALATLVVNKLRGTLQVCAVK
                     APGFGDRRKAMLDDIAILTGGQVISEEKGIKLENVGLQDLGKAKTVRVDKDNTTIVDG
                     AGDRKSLEGRVRQIRTQIEETTSDYDREKLQERLAKLVGGVAVISVGAATETEMKEKK
                     ARVEDALNATRAAVEEGIVPGGGVAYLRCLPAVQALSLEGDEKLGLNIVRRALEEPAR
                     QIAQNAGEEGSVVVEKLKGQTGSFGFNAETNQFEDLIEAGVIDPTKVTRSALQNAASV
                     SALMLTTECMVSEIPKEKEPAVPGGMGGMPHGGGMY"
     gene            complement(29405..29692)
                     /gene="groES"
                     /locus_tag="PLM79_05000"
     CDS             complement(29405..29692)
                     /gene="groES"
                     /locus_tag="PLM79_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOV85698.1"
                     /translation="MKLKPLNDRVVVRRTEEEQKTAGGIIIPDTAKEKPVQGEVLAVG
                     NGKLMEDGSRRPLDVKAGDRVLFSKYAGNEIKVEGEELLIMREDDILAVIE"
     gene            complement(29840..31546)
                     /locus_tag="PLM79_05005"
     CDS             complement(29840..31546)
                     /locus_tag="PLM79_05005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85699.1"
                     /translation="MKRKIQGLLLFRLLLGVFFLVLTLVVQSRREGDLLAARIQPLYF
                     FAMVIFLFTIVAAVSLKHIRHLERFGYIQLFFDVEAVTVLIFLSGGIESLFAFLYMPV
                     IISAALLLQRRGSLLTASMCSLSYGFLLDLQYFGWISPLQIVADTTHLRDSGTYFITI
                     LMSIAGFYLVAYLSGYLAEELRSSSRQVEKQKSDLRQLEALHRNIVESISSGLLTIDE
                     KGTVLFWNRAALETLGLKAEELRGRPLQEVFSSVNPVDWSREVPSSLSSAVRSTRRKE
                     IHYRRPDGEDLTLGYTVSVLQEGDGGVSGWIVIFHDLTHLKAMEDHVRRMERLAFAGK
                     IAAEIAHEIKNPLAAMSGSFQMLRGEIQADSIQSRLMDIVDREILRIKDLVNDFLWLA
                     KGSRKPGRIEDLSVCSAIREVVALLRSRERVSVLHRIRTVFDDGPTLSLDPHHFQQIL
                     WNLLVNALEAMPDGGDLSIRVSRSSRKDSPVPEARIDIRDRGQGISAEIRDRVFEPFF
                     TTKQSGTGLGLSIVYQLVENAGGRIEVTHHDEGGTTFSLFFPIPTSSPLQSNPRMIIS
                     TH"
     gene            complement(31604..32818)
                     /locus_tag="PLM79_05010"
     CDS             complement(31604..32818)
                     /locus_tag="PLM79_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV85700.1"
                     /translation="MPIYLWKGTDRAGKKQKGEMEADNPGIAQQLLTRKGINITSLKQ
                     KPKDLSEYLPFLQAKIKERDLVIFVRQFATMIDAGLPLVQALEILREQQENPTFRRIV
                     KQVKVDVEEGATLSDAIRKHPKAFDDLFVNLVAAGEAGGILDTILGRLAAYIEKVANL
                     KKKVKGAMTYPIIVVAIAIIVVAVILIYVIPVFAGLFKDAGAKLPALTLFVMNVSDFT
                     KAYFHWMVLGLIVLIFLLKRVRKTEKGRDITDRLFLKAPVFGILIRKVAVARFTRTLG
                     TMLSSGVPILEGLDIVAATSGNTVIERAIRKARAAIAEGRTVAEPLEETAVFPPMVTQ
                     MIAIGEATGALDQMLSKIADFYDEEVDIAVEALTSMLEPLMIVFLGVTIGFLLIAMYL
                     PIFQIADVVGRG"
     gene            complement(32873..34567)
                     /gene="pilB"
                     /locus_tag="PLM79_05015"
     CDS             complement(32873..34567)
                     /gene="pilB"
                     /locus_tag="PLM79_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOV85701.1"
                     /translation="MSKKIGELLVSEGLLDNDQLARALEEHNQTGERIGAVLIRLGMI
                     GEDTLVGFLARQFHVPEVNLAKLSVPKAVISLIPMDVAQKYQAIPFGIMGNTLNVAMA
                     DPGNLFVIDDIRFLTRKNIHVHVASETSIKKIIGQHYGADENIEDMLGMLKEEVDVDV
                     VESQEDVDLSFLEDAAEQAPVVKLVNLILMDAIRKQASDIHVEPYEKMMRVRFRIDGM
                     LHEIMRPPLALRNAIISRIKIMSRLDIAERRLPQDGRIKLKAKGKEMDFRVSVLPTLF
                     GEKVVLRLLDKSNLQVDMTKLGFEESQYKAFRSAIHAPYGMVLVTGPTGSGKTTTLYS
                     ALAELNKTSHNISTAEDPVEFNLPGINQVQILDAIGLNFASALRSFLRQDPDIIMVGE
                     IRDFETAEIGIKAALTGHLVLSTLHTNDAPSTISRLLNMGVESFLVSSAVNLVVAQRL
                     ARRVCTECRIEENVPPEALLDLEVREDELGSFACYLGKGCPVCNHTGYRGRIALYEVM
                     PMHDPIRELVLLGASSAEIKKESVRLGMVTLRRSGINKLKAGITSIEEVIRCSVKD"
     gene            34799..35086
                     /locus_tag="PLM79_05020"
     CDS             34799..35086
                     /locus_tag="PLM79_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HOV85702.1"
                     /translation="MICATVKAGSECAFMTKSGCGFNGGSCHPIVDQCEGCQRVVDLA
                     SGRYCNSYPNPAAKWRTSVCNFASHAKASTQAQQQAKINPLKASKRSTKRK"
     gene            35390..36622
                     /locus_tag="PLM79_05025"
     CDS             35390..36622
                     /locus_tag="PLM79_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOV85703.1"
                     /translation="MPRKDSNTVERTLILTGTQAAALAARLSRVQVITAYPITPQSKI
                     PEILSEYVEKGELQAEFVRVESEHSAMGVCISASLVGARVFTATAANGLAYMHEQLHW
                     AAGARLPIVMPVTNRGLGAPWTILNDMQDTLSQRDTGWIQFHCMNNQEVLDKIILAYR
                     VAEQIMVPIMVCFDGFRLSHTMMPVSVPPQDRVDAFLPPYRTPYELSPHNPVNINPVV
                     MSDPLPDSRGRLCPSYMGFRFRLQEELESALAVIADAGRRFGEFTGRFYDDVLYRYRA
                     QDADVLFVTMGSLSSEATDAADLLREEGVKAGVVGLGVYRPFPARELAEAVQNAKGIA
                     VLEKGISYGYEGALATELKAALFSHNGRRPTLHNYILGLGGKDVKPSDLTGPAREVME
                     SAHKGIRQEKPRWLGYGI"
     gene            36657..37559
                     /locus_tag="PLM79_05030"
     CDS             36657..37559
                     /locus_tag="PLM79_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV85704.1"
                     /translation="MSTTIMEFPDQEYILPGTRTCAGCGLPIAYRYILKALGPKTIVT
                     HPACCLTILHGIYPRTPVAVNAVNNTFASTAASASGLAAGLKATGREDYTVLAMAGDG
                     GTFDMGIQALSGAAERETDFIYVCYDNEAYMNTGVQRSSATPSGALTTTTAVVPKEQP
                     KKDFIQIMEAHHPAYLATTCSSFPGDLYNKFAKAKDIRGTRFVHLMVPCPSGWGFPTR
                     DTVKIGRLAVETGVVVLYEVERGGPIRLTGKSLSMARSGRKRPVEEYLRPQGRFKKMT
                     PERIALFQQEVDQRWAGLLQRAGF"
     gene            complement(37700..38713)
                     /locus_tag="PLM79_05035"
     CDS             complement(37700..38713)
                     /locus_tag="PLM79_05035"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOV85705.1"
                     /translation="MANRRTPRRNAYVRSVGRFLPERRMTNREFEDMVDTTDEWIVTR
                     TGIRERRILEPGLGSSYMAVRAARECLERAGASPEEVDVIIVGTVTPDMLFPATACLV
                     QKEIGASRAWGFDLSAGCSAFVFALTTGAQMIEAGRYDKVLVIGSDVMSTIIDYEDRN
                     TCVLFGDAAGAALLEPCPEEGYGVLDFVQYVDGAGGDFLHMKAGGSKRPASCETVKNK
                     EHFVYQEGRQVFRHAVIEMANVSAEILERNRLTGDDVALFIPHQANLRIIEASANRMK
                     IDRSKVIVNIDRYANTTAGTIPLCLYQAVVEENRVRKGDYLVMSAFGAGFTWGSALLR
                     WWE"
     gene            complement(38713..>38917)
                     /locus_tag="PLM79_05040"
     CDS             complement(38713..>38917)
                     /locus_tag="PLM79_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV85706.1"
                     /translation="SLARFVAERDGTTPEAVIRGWESQVPMGRLGTPEEFGALVAFLA
                     SEQAGYITGASIQIDGGYYKGIM"
BASE COUNT         7631 a        11859 c        11485 g         7942 t
ORIGIN      
        1 agcgccgtag tcgaaggcat caacaatcaa atcaagaccc tcaagagaca ggcttccggc
       61 ttcagggacc ataaatcttt taagctcaga ttgtaccctc ttcataccca aaggcactcg
      121 ttagccggat ggaccgaaaa tgacccggat cgagttcaag gtctaaccgg gttcgattga
      181 attttagatg tcaaccgaac gcaatttggt ataaaaaaac tcggttcggg gcggccgttg
      241 tgggcctttg gctgaagcgg tcttgcccgc ttccatccga ggtcaagggc gccttcccct
      301 tggacccggg atggagcggc atcggcccgg acgacatggt gcgcagctcc gtttggcttc
      361 caaaagcttc cgtttctctt gagacagcct agccgttgca acgcccgacc atcgacggcc
      421 gccgttgcca atccgttcag gcatttgaca ggaggaggcc atgcctcaca ggaaccggtc
      481 aaaagaaacg ttttcggcat cccccctggc caaggccccg acccacatca aggggttgga
      541 taccgttctg gagggcggtt tgcccagggg gcgcaccacc gtggtcaacg gcgggcccgg
      601 atcgggcaag accctgctgg ccctggaatt cctctatcgg ggcgccctgg ccggggagcc
      661 gggcgtcttc atcgggttcg aggaaccgct cgatcaactg agggtcaacg ccggcaccct
      721 cggctgggac ctggcggctc tggaacggga caggcggctg tgcctggtgg agggcaggct
      781 gaagccggac accctggtca gtggggaatt cagcctcaag ggcctgctcg cgggtgcttc
      841 gggggtgggc cgggagatcg gcgccggacg ggtcgtcatt gacgccctgg aggtggtcct
      901 gcgtctatac gactctctcc gtcaggtgcg caacgacatg cacctgctga acgattggct
      961 ccaggccgag gggttgtccg ccatcctgac cgtccggccg ccggttttcg ggagcgtccc
     1021 gtctttccat gatttttttg actccatggc tgactgcgtc atctacctgg acgcccgggt
     1081 ggacaaccag ttgagcaggc gcaggatcag ggttgtcaag tatcgcggct ccggtttcgg
     1141 cagcaacgag tatccctatg tgatcaccga cagcggtttg cacattgagc cgatttccac
     1201 cgtcggcctg cgtcacaaac ctcttgggga aagggtatcc accggtcttg cccgactgga
     1261 cgactttctg ggcggaggct accggagggg ggcctgcatc ctggtcgccg ggagccccgg
     1321 gaccggcaag acgctgctgg ccggcacgtt ttccctcgcg gcctgcggcc ggggggagaa
     1381 ggtgctgtac gtgggctacg aggaatccca ggaggccgtc atcggcaaca tgggccatgc
     1441 cggcgttttt ctaaagcctt gtgtggatac cggtctcttg ctgttccata ccgtcatgcc
     1501 ggaggccatg ggacccgagg agcatctcat ccgagtgatg aacctgattg aagcgcacaa
     1561 cccccggcat ctggtggttg acgccatctc ggcgtgcggg cgcatgggag ggaaacaagc
     1621 cgcttttgat ttcctggtgc ggttgctcaa ccggtgcaaa gagcggaaca tcaccgttct
     1681 tctggtcaat caactctcag ggaccacaac ccatatggag gtatcgggca acgacatctc
     1741 gtcgatcatc gatacggtgc ttttcatgtc ctacgtggag ggggcgggcg agatcaaccg
     1801 ggtctttcag gtgctcaagt ccagggggtc cggccactcc aatcagaagc aggaatacat
     1861 cattacggat gagggaatcc gtatcctcga accgtatacg ggcgaaggcg aggtcctgac
     1921 cggttccgcc aggaggcttc aggaagccag ggacgccgcc gaggcccaac gcctggccgt
     1981 ggcgatgaaa gccaaggaga tcgaactgga acagctcagg atcgagcagc ggcagatcac
     2041 ggaaagaaag cgttaccagg cgcaagtacg cggggccgcc cctgggagcg cggatgggga
     2101 ggatcggtca tgaccaagga cggatccccg tccggaacgg cttcccaaag acacggcctg
     2161 taccgatttc ggctctttgt cgccggaaac gaggtgaatt cccagagggc caaggaaacc
     2221 cttttccatc ttgccggaac ttatcttgcg gaccggcatg agattgttgt ggtggatgtc
     2281 atgcaggatt accaggctgc gctggactcc cggatcgtcg tggtgccgac cttgctggtc
     2341 gagagtccct tcgagcgggt cattgtgggt tctctcagcg atgaaaaggc ggtgttggat
     2401 gcccttggcc tggcgcccga gaggaggccc caatgagcgg ccgcaaaccg tcctatgaag
     2461 aactcgagac acgactggaa cgggcggaag cggtgctgga ttccctgcgc cggggagaat
     2521 ttgacctgat catcggcctt cgcgagccga tcgtcgtgca gttcaaatcg ctggcggaag
     2581 agaaagagcg gctggcgcgg gaatggcaga ccacctttga cgccgtaggc gacgccatct
     2641 ggcttctcga cggcgaccag cggattctgc gtaccaaccg ggcggcggaa aagttctttc
     2701 agcgcccggc ggacgcactc atcggccgac attgctggga gatctcccac ggcacgccga
     2761 gaccgatccc cgaatgcccg ctcttgcggt tgcgccggag ccttcgtcgc gagcaaacgg
     2821 aaatgccggt cggcgatcgc cttctttatg tgacggtgga cccgatcctg gacgcgggcg
     2881 gcaagttgac cggctcggtg catgtcgtca gcgacatcac cgagcgcaag cgggccgaag
     2941 agagaatccg tcggagcgaa gaaacttacc gcaacctctt ttgcaacgct caggtaggct
     3001 tgttcagaac caggatatcg gacggcaaga tcctggagag caacgaacaa ctggcccgca
     3061 tgttcggcta cgacactcgg gaagaattca tcgccgagta cgcgacgtta cagaattacg
     3121 tggacgccgg cacccgcgag aaaatgctcg aacgcattcg tcgggccgga agtatcgagg
     3181 actttgaagc ccgtttctat cgcaaggatc gatcgatctt ctgggcgcgc tattccgccc
     3241 gcatcgatcc tgaacagggc tggatcgagg gggtggccga ggatatcacc gaacagaagt
     3301 tgaccgaaga aacactgaag attcaactcc gcatcctctc cagctttcac acaatacccg
     3361 acgaagagat attcgacgaa gtcttgaagg tcatcctgga cgtcatgcac agcccctctg
     3421 ggcttttcgg ataccttgac ggcgcactgg tggtgccgac catgacctat cagatgtggg
     3481 acagctgccg gatagccgac aaaaagaccg tttttcctcc cgcccaatgg ggggacagca
     3541 gttggggccg ggccattcgg gagaagaaag ccaactacag caacgacccc tcgaccaaga
     3601 cgcccgcggg ccacatcccg gtggcgcgac atatcaacat gcccgtcctg ttccgggacg
     3661 aggtcatcgg cctcttgacg ctggcaaaca gcgaaacgga atacaccgaa gcggatgttc
     3721 ggaggttgga ggccatcgcc gatcaagtcg ctcctctttt gagcgcgcga ttgcagcgcg
     3781 tccgggcgga gaaagagaaa gacagactcc ggaccgaact cgcccaggcc cagaagctgg
     3841 aatccgtggg ccggctggcc ggaggcgtgg cccatgactt caacaacatg ctgggcatca
     3901 tcctgggcta cggcgacatt cttatgcaga agctccaccc cggggacccg ttgcgggatg
     3961 atgtgaaaaa gatcgtggag gcgggaaaac gttcggcatc gctgacccgc cggcttctgg
     4021 ccttcagtcg gaaacaaacc cttcagcctg aggtgctgaa tctcaattcc gttttgcgta
     4081 accttgaaaa gatgctccgg cggctgatcg gcgaggacat cgacctcaaa cttacgctgg
     4141 cggacgatct ggcgcatgtt cttatcgatc cggggcagtt cgatcaggtc atcatgaact
     4201 tggcggtcaa tgcccgcgac gccatgccca cgggcggcaa actcaccatt gaaaccgcca
     4261 atgtggccat cgaagagcaa atccaaacgg ctgtccctcc aggacaatac gtcatgattt
     4321 ccgtcaccga ttccggctgc ggcatggatg aaaccgtgtt gcctcacatc tttgaaccgt
     4381 tctttacgac caaggaaaaa gagaagggga ccggactggg actttccacg gtctacggca
     4441 ttgtcaaaca atccggcggg catattcggg cgtactcggg acccggacag ggctcgacgt
     4501 tcaagatctt tctgcccccg acccacgtga aaccgcaggt ggagaagatc tccctccaag
     4561 agcgaaggca aatcggcggc ggcaagcgcg tcctggtggt agaggatgag gagagcatgc
     4621 gcgagctgct ccgaacgatt ctttcaaggc tgggtttcga ggtgttcgtt gcggccaacg
     4681 gcggggaggc ccttttgcgg gtggaggagc agggcctgga acccgatctg gtgctcacgg
     4741 acgtggtcat gccgggcatg agcggggccg tgctggcgga acgccttcgc agaagccaac
     4801 ctgatctcaa agtgcttttc atgtccgggt acaccgacga cgccatcgtt caccacagaa
     4861 tgtccgacca tgggacccct tttatccaaa agcccttttc cattgacgac attgctgaaa
     4921 agatccgccg ggtgttgcag aacggataga ctgttgcccg cccagccatt cagacggccg
     4981 cggccgacgc cgtgccgctt gatccgagaa cggtgggcaa gttccgcgtt tttccttctt
     5041 ctacgagaca ggcaaccacg gcgatttttc gtccgaatcc gttgaacgat gccgagaaga
     5101 cccccgatgc caaaaaccag gcactgacgc agacatccgt gtcgaaggga tgctgtgcgg
     5161 cgatccggca ccttgaccca ggcaggttgc atcctcgtga cccgtgtctt caatgaacgt
     5221 tcgcgcaccg ggatcgcccc ggccgtcacc ggccggtctc agcgtgtcgg atttcccctc
     5281 gatccggaac ttgccacgaa cgatttcccc aaacctcccc gagggcagat cactcaaacc
     5341 cgtacactct ctgcagagcg aaccggcagg aagatcggcc ccgtattccc ggaagaaccg
     5401 gacccaaatt cttgaaggcg taaacgagat cctgggaagc cagcccaagg caagatcgtc
     5461 aatcccgtct cgtccacctg gcggcaaaaa atccgtccac cccgtgccgg tgaggccagg
     5521 tgcgaaagaa cggcccgccg ctcatggaac gacagctctc gggaaggacc tcctcggcgg
     5581 gcagcacccg atactccccg tgggtttccc cgaaggccag ggccacttcc tcgttttctt
     5641 cgggaaagag ggtgcaggtg gcatacacca gggaccctcc gggacggacg aacttcgccg
     5701 catgatcgag caaggccttc tgttgccggg agaatcgata ggaatccttg ggatgcctac
     5761 gccacttgat gtcgggcttt ctgcgaaccg ctccccaccc cgagcaggga gcatccagca
     5821 gcacccggtc gaagacgccc aggtcttcgg gacgcagatc caggacgtcg ccccggacgg
     5881 ggcgaaggat gtgaagccct tgtctcgaag cattttcccg gaggtcctcg agcttccatg
     5941 ccgattggtc cacagccagg atctcgcccc gatcttccat gaggattccc aggtgggttg
     6001 attttccgcc gaacccggaa cacaggtcga gaattcgctc tccgggccgg ggagccacga
     6061 gatgggacac cagctgggag gcttcatcct ggacctggac ccagccgttt tcgcagacgg
     6121 gcagccgcgc gggatcctga cgaattcccg cgatccgaac cgccccggga aggtagggag
     6181 actcctcggc cgcaattccg tttgtactca gccattccat cacccggggc ctgtcggcct
     6241 tcaaaggatt gatcctcagg gacaagggag ccacccggtt gttggcccgg cagatctccc
     6301 gggtttcctc gagtccccag gaccgaacac attgttccac gaaccatcgg gggtgggagg
     6361 ttgtgacgct gaggtgttcg acggggtcgg tctcctcgga gggccactcc cagtgatccc
     6421 cccgcctcga agcttcccgg aggaccccgt tgaggaacct caccacgtag ggaggctgag
     6481 agctcttggc gatctccacc gtctggtgga ccgccgcgtg gggcgggatg cgatccagca
     6541 tcaggacctg gtaaagtccg catcgaagaa ggacccgcac ggcggggctg atcttgcggg
     6601 gcttcagggt gctcaaccgg tcgatgtgcc aatccagccg gccctgccat cgcagcacgc
     6661 cgtacacgat ttcggagagg agcgcccggt cccgttcgtc catgccggga tggcgggcaa
     6721 gggtcgtccg taggaggcgg tccgggtggg ataccttttg atccagatgg agcaggattt
     6781 gaaccgccaa aatcctgact ctcatcacga tccttccggc ttcagggacc ggacttcccg
     6841 gaggatggat tcgacgtgtt ccagcccgac ccgggtatcg aagcaggggc cgaagggcct
     6901 ttcgttgaag atgccgtaca cgggcagggg cgtggtgtct tgaatgccgc tggtcagatc
     6961 ccgctcacag gcaacggcga tgatgaggtc cggcctcgtt tcgatgacga tcctgcgggc
     7021 aaaggtgccc cccgtggcca cggcaagatc caccccgtac cgggacgcca gctccagcag
     7081 gtccttgatg ggacacttgc cgcaccgttt gcagttctcg acccggtagg tgatcttcac
     7141 gggacaatcg tggttctgga ggcagtgggg catgagcagc aggatctttt ggggaggatg
     7201 accgttgcgg cactgggcca tgaccagttc gttgttcaat gccacgaagg cgtgctgcac
     7261 ttcctctttg cgaagcccga ccagtcggcc caccttgatg aggaccggca ggagcagctt
     7321 gatgacaagg cttcgcagct tttgggaaaa gggcacgtcc ctgccgagga gggtcaccac
     7381 gatgaggagc acgaaggcga ccaggaccag gaggcccact cccccgatga gaagggccat
     7441 gaggatggga aggctctggt gaatccggcc gaagccgtag taaggaatga agtaggacag
     7501 cccgagaagg gcaaggatga ggaccgcggt caggccgaac aggcctacca gggtcatctt
     7561 ttccgactgc ctggcggaag ggagattttc agatggttga ggtttcgctg ggctcatgaa
     7621 gcaggaaatc cgtcgctttt gcggaagaac cgccgaccgg ggccgttgcg gtccgccggg
     7681 acgcggaacc gacctgccgg ggagacttaa accgggatgc ttatcgaagg atcgttcccc
     7741 cggggatgaa gtggcctcga agaaactcga ccgccgccag gcggcgctgc ccttccagtt
     7801 ggagctcccg gatgcacacc gtccttccat cccccgccac aacgagcagg cccgcgccgg
     7861 tctcccccag cacttccccc ggggttcccg gactgctcag ggcggcaagg gaagccttga
     7921 aacacttgac gcgccggtct tcccaacggc aatgggcccc cggccacgga tcgaaggccc
     7981 gaatcctgtt gaccacctcg agcccgggcc gggtccagtc gaccttgagc tcctctttcc
     8041 cgatgggcgg tgcgtaggtg gcgagaccct cgtcttgggg tcgggccacg attcgtcccc
     8101 gtttccaatc cgggagagtc ttcaccagca ggtccgcccc cacctccgcc agcctgtcgt
     8161 gaagcgaccc gaaggtgtcg gactcctcga tggagatcgt ttcccgggaa aggacgggtc
     8221 ccgtgtccat cccggcatcg aggagcatga tgctgacccc cgtcgtccga tcccccgcca
     8281 ggatggctct ctggatgggg gcagccccgc ggtaggcggg caaaaccgat ccgtgaatgt
     8341 tgagcacccc caatgggaag agatcgagca actcccgggg aaggatctga ccgtacgcca
     8401 ccaccacggc acattcggcc ccggtggagg caatggttgc caccgcttcc gggtcccgaa
     8461 ccctcgcggg ttggaaaacc ggaattcccg ccccctcggc gaaagccttc accggagaag
     8521 gactggtctt gcgtccccga cccttgggtc gatccggctg ggacaccacc agcagcaccg
     8581 ttcccttctc ggccccaagc cttcggagag tcggaacggc aaaatcgggc gtgccgaaaa
     8641 aaaccagctt cggcaactcc cggttcatgc ctgcccttcc ttctgcttct tcttccactt
     8701 tctcttgaaa atatctcggg tgatcggtcc cagccggtca ataaagagtt tcccgtccag
     8761 gtgatccatc tcatgctgca acacgacggc aagcaatccc gaggcccgga tttccaccgg
     8821 cttcatatcc cggtcgaagc cccggaccgt gatctctcca taccgcttta cggcggcaaa
     8881 atactcgggc acgctcagac acccttcttc gtagacgatt tctccctggg actccgtcag
     8941 ctgcggattg accaggacaa tgaggccgtc gttgttttcg ggcttctgaa gatcgatgac
     9001 caggactcgc aggggcctgc ccacctggtt ggccgccagg ccgatccccg gagccccgta
     9061 catggtctcc gtcatatcgt cgatgagcct tcgcacctcg cggtccattc gacccacctc
     9121 caccgccttt ttgcgcaaga tgggatcggg atacttacgg atctgcaaac aacccattcc
     9181 ttgtcctcga acggatcgat ggtcaaaaaa cgtcaccggt aagcaaaaat cggcatttct
     9241 tcccggagtt gctcggccga gcatacgctc gacaggttat tctaaatttt tggaggcggc
     9301 ttttcaatgc gtatgtcgct ccatttgcgg acctgcgggg ctcaggcgcc caggccgtca
     9361 atcttttgac gacaggcagg acaagcgtgg tcctcgtcga gccgggttcc gcaacggcac
     9421 atccagccga cctgccgtgc ggggttcccc accatgaggg cgtggtcggg aacgtcccga
     9481 accaccacgg ccccggcccc gatgaaggca taccgtccga tggtggtccc gcagacaatg
     9541 gtgcagttgg cccccagggt ggcgcccttc ttcacccggg tcggccgcaa ctcgtccatg
     9601 cgccggatgt gagcccgcgg gttgtagacg ttggtgaaaa ccatggacgg gccgcagaac
     9661 acctcgtctt ccagggtcac tcccttgtag acggacacgt tgttctggat cttgcacccg
     9721 tccccgatgg agacctcggg tcccacgacc acgttctgac cgatgctgca gtttttaccc
     9781 acgatggacc cggaaagaat gtggctgaag tgccagattt ttgtccccgt gccgattctc
     9841 acccccggat ccaggacggc cgaagggtga gcgaagaaat cgggctcgac ggcgggggct
     9901 tcggggaggg gcttcccctc ggggatgaag gggtcctcga ggggcaaacc cgcgggcacc
     9961 ctcgtttctg ccgcttccag cggagcatag gaagacggtt cccctcccgc catggatttc
    10021 acggcggcga tgggcacaca ttctccgtat cgattgaggg acctctggga agcgttgagg
    10081 acccgaagaa ccgagaggcc ttcctccccg tcggtggtgg gtcgtgcgcc cgtgcggacg
    10141 cattggagaa agtgcgcgca ttccagcttg aggggttccg attccgggag ctcgacccgc
    10201 tccggatcgg ccttggccgg aaccgggacg ctcttttccc agcggatctc gtgagcatag
    10261 aggaggagct tgtccgacca cggcttggtg tcgtcgaaga ccgccatttt gcggtccccc
    10321 accaccacca gcttctgttc cttgaaagga tggagccacg aaacgaaaat gtgcgccttg
    10381 aggcccgagg gaaactcgag gtgagtggtg gtcacatcgg cgatcttgcg gtgaaggtag
    10441 tttccccccg tggccagaac ggtctcgggc tgttccttgg ccagggagag aatcatggag
    10501 atgtcgtggg gagcaaaaga ccagaggatg ttttcctccc ggcggatttt tcccaggttc
    10561 aaacggtggg aatagatata gttgattcgt cccagttcgc ccgaatcggc cagttctcgg
    10621 aggcgaacaa aggccgggtg gtactggaga aggtggccga ccatgagggt gcgcttttgg
    10681 cggcgggcca gtccgatgag ttcctcggcc tcgggttcgc tgagcaccag gggcttttcc
    10741 acaaaggcgt gctttcccgc gagaagggcc tcgcggacca gggcaaaatg ggtctcggcg
    10801 ggagtggcca cgaccaccgc gtcgatatcg tttcgggaaa gggcttcatt gaatgccaaa
    10861 cacagctcga cccgagggta gctctcctgc agatgggcca ggaggtcctc gttcttgtcg
    10921 caaatcagcc tcaaggcccc gagctgggca tagttcctca caagatttcg accccagtag
    10981 cccgccccga tgacggcaac ggccggtgct tccgcattct tcggggcaga tggggattcc
    11041 attccacacg atgactggtt cataggcgct ccatgcatgg gttctctgag atggggaaag
    11101 gagcgggtgc ccagaatctc tcgccgcaga atgcatatca cagatgcggc ttatattgga
    11161 acgaattctg catagttcta cggagcactc acgaacaccg atcttccctg cgagaagaaa
    11221 ctgagggcca acttccagga gaaagtgatt ttgcaccggg tcctccccac cgaatcgggt
    11281 ggggaatcct gcgagacact atcgaggcaa agaaccatga gagagggcaa gcgaatcggg
    11341 gttgtcgttc ctgcctacaa tgaagaggcg ctcatcggcc gcgtcatcga gacgatgccc
    11401 gcctttgtgg acggaatcta cgttgtggac gactgcagca gggattccac ctgtgagaaa
    11461 gtgctctcct ccaaggccgg catctcggaa aatcaaaggc ttcatctgat ccgacacgaa
    11521 gccaaccaag gggttggggc tgccatcacc accgggtaca ggagggccgt cgaggacggc
    11581 atggaggtca tcgccgtgat ggccggagat gcccagatgg accccgacga actggataag
    11641 atcgtggatc ccgtgatccg cggggaagcg gattacgtaa agggaaaccg gctctttacc
    11701 gggcaggcat ggcacttgat tcccaagtac cggtacatgg gcaacgcgtt tctttccctc
    11761 ctgaccaaag tggcctcggg ctactggcac gtggccgatt ctcaaaccgg ctacacggcc
    11821 atctcggccc aaacccttcg gtccattccc ctggatcttc tctaccctcg ttacggttac
    11881 cccaatcacc tgctggtcat gttgaacatt tacgaacagc gggtcaagga tgttcccgtg
    11941 cgccccatct acaacattgg agagcgttcg gggattcggc tgcgcaaggt gattccccgc
    12001 ctgtcctggc tgcttttgaa gtgtttcctg tggcggatga aggagaaata tatcatccgg
    12061 gatttccacc ccctggtatt cttctacctt atgggattct ctcttctgcc cgtgggcatg
    12121 gtgttcggga cctatctgtt tatccatcgc ctgctcgacg gccccgtggc cgcaaccagt
    12181 gccttgtttt cggtgtttct tgcggttacg ggcctccaag cattgttttt cggaatgtgg
    12241 ttcgacatgg agtacaataa agagctcaaa gtccggtaag gaagcaacga ggcgattctt
    12301 tgcgatcgaa actgccgtgg agaggctgat gagggggagc gggacgcatg gatcccgacc
    12361 tcgggcagca tgaggggatg agaggacgta tggatctgaa gaacaagaga ttcctgtgta
    12421 tcggcggtgc cgggttcatc ggttcccata ccgtggacgc ccttctgaaa gaagatgtgg
    12481 ccgaggtgcg catcttcgac aatttcaccc ggggaagtcg ggaaaacctg gccgaagccc
    12541 tcgaggaccc ccgggtgagc gtgtttcctc tgggaggaga catcctccat acggacatcc
    12601 tggacgccgc catggaaggc atggacggcg ttttccattt cgccgctctt tggctgctgc
    12661 actgctacga tttcccggaa tccgctttcg aggtgaacat tcggggaacg ttcaacgttt
    12721 tgcagtcctg catccggaag ggggtcaaga gactggtctt ctcttcttcc gcttccgtgt
    12781 acggagacgc ggtccgggaa cccatgaatg aagatcacgt ttacaacaac aagaccttct
    12841 acggcgcgac aaagattgcc ggcgagcacc tgtgccgggc cctgtatcac cggtatcaag
    12901 gcacggatcg gcatttcgat tacgtcggtc tgcgatacat gaacgtctac ggcccccggc
    12961 aggactacca cgggacttac atcacggtca tcatgaagat cctggaccgc ttggaccagg
    13021 gtctgcctcc cattctgtat ggtgacggct cccagtccta cgatttcatc tacgtgggcg
    13081 attgcgcccg tgccaatgtc tgcgccatga aggcggattc cacggacagc ttctataacg
    13141 tggggaccgg caggaagact tccctcaggg aaatcgcgga gctcctcctg gaactgaccg
    13201 gatcccggca ggaaatccaa tacgaagaag ccgggctcac tttcgtaaag aaccgggtcg
    13261 gctgccccaa gaaggcccaa tcggaaatct ttttcacggc cgataccgac ctgcgccggg
    13321 gactctccca gttgatccaa tggcgggccg gccacaagga gcacgtgaaa cgtctccgtg
    13381 caggcaaggg gtcggtgcgc ccatgaagcc gaaaaacatt cccatcgccg tccctcacct
    13441 cgaagaggag gaatggctcg ccctccggga acccctcgaa acggggtggc tcacccaggg
    13501 accgaaagtg gccgctttcg aaaaggcgtt tgccgcccgc cattcggcaa agcatgccgt
    13561 cgcgactacc agttgcacca cggccctcca cctaattctt gccgccctgg agatcggtcc
    13621 gggcgacgaa gtgattgtcc ccgccttcac ctgggtcgcc acggccaatg tggttctcta
    13681 ctgcggggcc gttcccgtct tcgccgatgt ggaccgcgcc acgtacaacc tcgaccccgc
    13741 ggaaattccc gggaagatca ctccacggac tcgggccgtc atcgtagtac accttttcgg
    13801 gttgtgtgcc gacgtggatg ccgtcgccaa ggccgccccg gggattccca tcctcgagga
    13861 cgccgcctgt gcggccggca gcacctatcg ggggcgttcc gccgggtgcc tgggaagcgc
    13921 gggagccttt tcctttcatc cccgcaaggt gatcaccacc ggtgagggag gcatgatcac
    13981 gacgaattcc gaggaactcg cccaaaagct gaccgttttg agaaatcacg gggccgggat
    14041 ttccgaggaa gaacgacacc gcggcccacg cccttacctg ctgcctgaat ttagccattt
    14101 gggtttcaat taccggatga cggatcttca aggggccctg ggcctggtcc aactcgggag
    14161 actggacggg ctcatcgccc agcgtcgcca atgggccgaa tactacaacc gggcactcgg
    14221 gggcatcccg tggcttcatc ttcccgtgcc gcccccgggc tacggcacca attggcagtc
    14281 ctatgtctgc aggatcgatg caggggcccc ctgctcccgc aacgagatca tggaaaagct
    14341 gcagtctcgg ggcatcagta cccgcccggg gacccattgc gtccccaccc tggattacta
    14401 ccggcgccgg ttcgacctgc gccctgaaga cgtgcccgtc gcctgggata gcgaacacca
    14461 gaccatcgct ttgccgattc acagccggat gagcccgcag gattacgacc gcgtggtcga
    14521 ggcccttcgg gagctgggtt gacgcctatg tgcggatttg tcggggtgtg caatcgcaat
    14581 ggggagccga ttccccatgt gttgctcaaa gagatgaccc gggtgattgc ccaccgcgga
    14641 ccggacgggg aaggtcatta cctggacgac tccgtggcct tgggacaccg tcgactggcc
    14701 atcatcgatc tgtccccggc gggtcaccag ccgatgacca atgaagacgg caatgtcgtg
    14761 ctggtctaca atggggagct ttacaactat cagaagcttc gggctcagct tgtcgcattg
    14821 ggacatcgtt tccattccca gaccgactcg gaggtggtgg tccacgccta cgagcaatgg
    14881 ggacccggat gtctggagta tttcaacggg atgtttgcgt tcgccgtctg ggaccgcacc
    14941 cggaggcgcc tcttcctggc ccgggaccgg tacggcatca aacccctgta ttggtatgcc
    15001 caaaacgggc tttttctttt cgcctccgag gtcaaggcca tcctggtaca ccctcgagtc
    15061 tccccggaag tatgctaccc ggccctcaat gaatacttca cttttcaaaa catcttctcg
    15121 gatcttaccc tattcgaagg catccgcttg ctgccgcccg ggagcagcct caccctggac
    15181 gtgcgcgagg ggaccgggcc ggtcctgcac aagtattggg actacccgtt cgatggggga
    15241 aagctgaacc tttctccccg tgaaagtgcc gacgagcttt tccggttgtt tatccaggcg
    15301 gtcactcggc aactggtcag tgatgttccc gttggagcct atctgagcgg gggaatggac
    15361 tcgggggcca ttaccgccac cgccgttcga catcttcccc ggttgacgac cttcaccggc
    15421 ggattcgatc tcacgtccgc atccggattg gagctggctt tcgacgagag acccgtggct
    15481 gaaatgatgg ccaacctctt caagacggaa cactacgaag tggtcatgca cgccggggac
    15541 atggaatggg tgctgcccga gctcatctgg cacctggaag acctgcgtgt gggccaatcc
    15601 taccccaatt attatgtcgc ccgacttgcg ggcaaattcg tcaaggtctc cctggccggg
    15661 accggcggag acgagctctt cgggggctat ccctggcgct attaccgatg cctggataat
    15721 gcctgtcgcg aggactactt caagcgctac tatgacttct ggcaacgtct tgtcctggac
    15781 aatgaaaaac cccgtctata caattccgat actcgaaggg gaatcgggga ccattccacc
    15841 ttcgaggtct tccgacgcgt atacgaccaa tggaccctgc cgcttaggga aaacggcgat
    15901 tatgtggatg cgtccctgta cttcgagctg aagacgttcc tgcacggact gctggtggtc
    15961 gaggacaagc tcagcatggc ccattccctc gaaacacggg tcccctttct tgacaacgac
    16021 ctggtggaat ttgccgtccg gatccccgcg caccacaaaa ttcgggacct ccagtgcgcg
    16081 gcacccatgg atgaaaacct gccgggaaag cgcaagctct acgacctgca gacagccaac
    16141 gggaaagtcg tgttgaggga ggccatgagc cggatcgtcc cccgggaggt cattcaacgt
    16201 cacaagcagg ggttcagcgc tccggatgcc agttggtttc ggggggagag catcgattac
    16261 atcaatcggc ttctcaggaa ccccaaggcc ctcatttatg aatttctgaa ccctgccttc
    16321 gtgggggaag tcttggacca gcattgttcc ggcaaggtca accggcgtct tctcatatgg
    16381 tcgctgttga gcttcgaatg gtggtgccgc tgtttcctgc acgagcctta ctccaaatgg
    16441 aggccccatc cggcaggcga gacccgacga ggagtgagga tgtgacgccc gcagcccgcc
    16501 tgtttgagtc gttgcggcac ctctatgaaa agcaccggct tcgcatgaag aagaggtggg
    16561 atcgggacct tccgttcgac gaactgcttt tcgaccgatt ccagcgtgca cgaaaccttg
    16621 gtttcggcga gggatccagc gtctaccaca acagctatgt ttatggggac gtgaaagtgg
    16681 gaagatccac ctggatcggt ccgtttaccc tgctcgatgg gaccggagga ctggaaatag
    16741 gggactggtg cagcatcagc gccggggtgc agatttatac ccacgattcc gtaaaatggg
    16801 ccttgtccgg agggaaactc ccttacgagc gcagccccgt cagggtcgga aaccgttgct
    16861 atatcggcag caagacggtc attgccaaag gagtgaccat tggggaccaa tgcgtcgtcg
    16921 gcgcatgttc ctttgtcaac aagagtctgc ctcccaattc catcgctgcg gggatcccct
    16981 gcaaggtgat cggaacggtg cggaccgacg acgagatctc cttccattac caccaagtac
    17041 ccgaatcttc ttgatccccc ggggtcacag gatgctccca tgcgaatctg ttgggtcacc
    17101 acctcttttc cccggtttgt cggggatcat tccgcaacgt ttcttctcac tctcgccaag
    17161 cacctcgtga ggaagggggt cgatctgacg attgtggccc cccacgaagg gggactcccg
    17221 gaagaggaga ctctcgaggg agtccgtgtc tgcaggtttg tctactggct cccgcgccgg
    17281 tcgcaagcgt tggcttacgg acacggaatt cccgacaaca tccggcgaaa cccgtcggta
    17341 ggcctccagg ttcctccatt tctggcgtcg gctgcgctga aggtcctctc catggcccga
    17401 ggggcggacg tcctccacgg attctggacc ccgagcgcct tcctggccgt cccggcgaag
    17461 aagaggtacg gaatccccct ggtcacgacc cttctgggca gcgacattcg caacgggccg
    17521 aagacggtca acaggattgc cctccatttc tccgacgccg ttgtctgtgc gacagaagag
    17581 atggccgcct acctgagaac ctacccggta aaggtcccgg tttttgacgt gaagcagaca
    17641 aacctcgtgg accccgaccg tctgcgctca ggggattccc tcgaacccga cttgctggaa
    17701 tggatcggga agggttccgc gttgattacc ctggtggccc gcctagtgga tttcaaggac
    17761 cccgtgggat tcgtgcacgc catgccgtat atcctccaag agcatcccgg cacccgtttc
    17821 ctgatcatcg gggacggtcc tctgttcgag cccttggaga cactgatccg gtcgatgaat
    17881 ctcacgagtt gcgcacgact gaccggacac agaaccgaca tcggctcttt cctagaacgc
    17941 tccaccgtct ttgtagccaa cagccccgtg agcaattgtt acagctgtgc cgttttggaa
    18001 gcgatgtccg ccggagtccc ttgcgttttg acggatgcgg gcgaccctcg aggtaccttt
    18061 cgaggctacg tggaattggc tcggcccaga gatccccggt cccttgcgca agccgtgaac
    18121 acgctcttgt ccgacgagga acaacgtcgg catcgggctg cgacttcccg gcaattcctc
    18181 ctggatctgg ggtttggttc cgaggtcgtg cttcggcaga cgatggagat ttacgaggct
    18241 ctccgtcgag accccaaact cccggatcgg ctctcgattc ccagagtcga cctatccccc
    18301 ggcatctaga ccgtgcgacg cactgaacat gggcacgcac ttcaaatcaa cctgggtcaa
    18361 agggcttaag agatcgcttc tggccctgac cctcgtgttt gtgggatttg ccctctaccg
    18421 atccgttctc aacgccgact ggtcactggt ggaggtccgc ccgggccggc tgtttctcgg
    18481 gtttctcctg atcgcggtgt ccaccgtcgt cggatcatgg atcctcggct atatctatcg
    18541 gaacctgggt ttgcagttga ctccctgcca ggccctcgag ctactcaccg tccccgctct
    18601 gggcaagtat gtgcccggca aattcctgtc catagccgga cacgcagcca tggccctttc
    18661 cttcggcatt cccttgcccg cagggaccgt cgccgtcggc gtagcgacca gcatagggct
    18721 ggctgcatcg accctgctgg ggctcctggt tttcctcctg tcgccggatt ctttccaagg
    18781 cctggtccga atcggttggc cggaatgggg aatcctcctc gccctcttgt tcctggtttt
    18841 gtgccccgga ttgtatcggc aagtcctcca attctttctt cgaatcttca agcatccccc
    18901 ccttcccttc catctctcct tcgggaccct cgcgcttctg ttgacgacca acatgctcca
    18961 ggcaggtctg cacgttacgg ggtttgcctt tgtggtcacg ggaatgcttc acctgccggt
    19021 ccacgcattg ccgaccatcg ggggggccct gtgccttgcc aacgtcgtcg gtttcctggc
    19081 ccttttcgcc ccgggcggaa tcggagtacg ggaaggaatc ctgttgttgc ttttggctcc
    19141 ttctttgggg gatgccccgg caggatttgt cgccgtggcc acacgcctga tccaggtcgc
    19201 cgcagacctg ctggccgctt ccatgggagc aacggttgcc ggggtggagc ggcgccgaaa
    19261 cgagaggtcc gacccagccc aagcccccat gcaggcagga cggaccggca aaccgcccag
    19321 ggcctgattc gaaacgccct cgggattcga ggtgtcgatt cttgtcatcc gaccgacgta
    19381 ttttgtcagt cttcgggtct ccaggcgtcc cacccacagg tggctgcagg gatatatcga
    19441 ccctgtgggc cgccagggcc accgcggcaa gcggaggttt tccccgtgag ttgagcgatt
    19501 ccgagagcac cagggcctta tgcccgcgaa ggcggtcatc tgttgggcat gcacgctctt
    19561 tccggcttcc agcctgcgcg gaaatgacga aacgggcttc gtgcccatcc aatcgctcaa
    19621 tcctggtttc ccgtctttgg gaacccgccg gacgcggccg tcgccgtgaa tagctcggga
    19681 caatggggga atagaccatg ggcttcccat ctgtgggtcc ggttatttcc cacttttttc
    19741 ttcaagggag ggtgggaaag agccgatcct tctcgaagga atcaggctca attcttgctt
    19801 cataaccggg gatcaacaag gtcacccagc catgcgtaac ccgtcggcgg atatggaaca
    19861 acttcacggg aaaggaggga ggaagcgcac cagagaccgg atttacccga tcacggggag
    19921 tcatttcagg aaaccttcaa acgaaaggag gaaccaagat gttcattaag atgaaagaga
    19981 gcaaaggttt taccctggtg gaactgatga tcgtcgtggc catcatcggg atcctggcgg
    20041 ccgtggccgt gcctttttac cagaggtacg tgcagaagtc ccggctgacc agcttggcct
    20101 tccccgccat gcactccatt gagaccaacc tcgcgggcta cttcgccatt cgaaacacct
    20161 ttgccggtgt gtccatgacc gcaatgatcc aggatgcgga gatgacctgc atcgtgaaga
    20221 acccgacatg ggaccaaaac tcaagacttc tcacttttgt catcaatact caagccagct
    20281 gtcccaacct ggcccagttt gccggaagaa ccatcaccgc gagagcaagt gtgacgggaa
    20341 acaagatcca aacctgggtc ctgggagggg ccatcgccaa cgagcttggc ttgaaatagc
    20401 ccctcttcct cccgtgaaac caaaggaagg ttgtggcgag atgcgcacaa ccttcctttt
    20461 ctttgtggtt ccacctcatt tcaggaacgg tcatggaaga aagaaatccc atcttcacgc
    20521 agaataagaa gctctacttg gcggctttgg gggtcgtctg cctgatggtc tttgcggcgt
    20581 actgcaacac cttttatgct ccccccgccc tggacgattt ccattctttc atcgacgaac
    20641 ctgcagtgag gatcaacagc tggtccatcg aggacctgcg gcggctttcc accacgatgt
    20701 tcggttggag tcgatggctt cccatgatca gtttcagttt ggacatctgg gtgggggagg
    20761 gaagcctgtt ccaattgcac ttaacgaatt tgctcattca tatttctacc ttcctggcgg
    20821 tcctttttct cgttttcgcc ctgtcccgcc acgccaagga gtgcccgtct acttcggaat
    20881 ccctacccga tccgtcgacc ctcgcgttgt gggtcgccgg gttgtgggcc ctgaaccccg
    20941 tgcataccag cgccgtcacc tacctggttc agcgcatggc agcaatgatg gcgcttttct
    21001 ttgttttgag cgtcgccttt tacgcggccg ggcgcttgtt gatgctgcgc agaggagggt
    21061 tcagcgccaa ggcgggcctg tgctttctgc tgagtttctt gaccatgatc atgtcgtttt
    21121 tttccaagca gaactccgtc atgctgccgt tcacgctggt gtgcgtcgag acctggttct
    21181 tcagacctca actccccggg gaagtatgga atttcctgaa gcgccgtcga atcctgtccc
    21241 tcatcgccct gggaattata ttgatcgttt tgtactacaa gattccgccg ttgctggtgg
    21301 gctattccca tcgtcacttc accctgaccc agcgtctcct caccgagctt cgagtcgtgg
    21361 tctggtatat ttcggtgatc ctctggccga acccgggaag gctctcgttg gaacacgaca
    21421 tccccttgtc cacatccctg attcaccctc cctccaccct ggcttgcctt ctggttctcg
    21481 tgtccatgat cctgtgggcg gtgcgcaagc gtcaacggtt ccccctggct tccttcggca
    21541 tcgtgtggtt tttcctgaac ctggcggttg agtcttcgtt cctgcccctc gagcttgttt
    21601 tcgaacatcg tgtgtatctg ccttcggtag gcctcatgct gagtctggtc gtgttggtgt
    21661 acacgggggt acggaaagct gtgcccgagg caccgcggga aacttttctc aagctgggat
    21721 ggtgccttgc ggccatcctt gcatccgccc tcaccctggc gaccttctca cgaaacaccg
    21781 tctgggagaa ctccgtggac ctggcccggc acgacgccca ggtgaacccc aactgtcccc
    21841 gggctcacgt gaacctggcc gtggcgctca atcgggccca ccggttcgag gaagctcttg
    21901 agtcggccta caaggccctc gaggtcacca ggcccaacta cgaagcgtat tccgtcgccg
    21961 ccaatgccct cgtcatcgcc cattcccagt tggggaatta tgaaaccggc atccgtgagg
    22021 cggagcgaat cctccgagaa accccgccaa aggccgacgc cggagcggca ccccttgcac
    22081 tgatcaatat cggccgggcc caccggaaga tggggaactt ggagcagtcc taccaggccg
    22141 ccaggcgcgc cctgaccacc catcaaagac tcactcccca gaagattccg gaattcgaat
    22201 tgctggtcat gtccctgctc tccgatgtgt atgtcgacgc cagggaggga aacattcccg
    22261 tggattttga cgaggacggg gaaccggatt cgctcgagat gcccaggatc gtctggatgg
    22321 ccgagcaatt tcacaagtgg aatgaccggg aaactgcgcg aacccttctg acacaggcgg
    22381 ctgcggacca tccggacgac ctacgctcgg ccgaactcct gagccaatac cggagggagg
    22441 acgagatgaa ccgtatccaa gaggaaaaga aggacttcac cagcaagtat ctacgctggc
    22501 cgtcggatcg cttccgggcc tgcatggcca tcgcttttct aaccgaaaag aagaaactcc
    22561 cctccttttt tgccggcatc ggcgaagcgg ccctggacta tgcccttcgg ctccggcccg
    22621 actccgcgga cgctcatctg ctcaaggctt ggttctgcta caaccaggac aaatccgagg
    22681 aagcgatcgg ccgggcacaa aaggccctcg cgctggatcg cgactatgcc cgggcctggc
    22741 tgggactggg cttcttcctg gccaaggccg gccggaccga agaagccttg gatgcgtttc
    22801 agaaatgcct ggacctctac ccggggaacc cggaccgggt ggccatcacc accatcgcgg
    22861 cggagctcaa atccaaattt gagggaaggc aggcggtcaa cgatcccaag cgggaatcat
    22921 cggcccgaag accctcgccg gaaggctttc accccggtta gcccgtcccg ggatcgcttt
    22981 tttcctcggc tgcgcgatta ggacgttccc atggccggag cgggttgcgg cgggtcgtcg
    23041 cagcccctct tttttttgtc tgcggtccgg agccgggcgc ttttgatcca ttcttttctt
    23101 gcggttttcc tcgggaaatg cgaatttatc gagcgcatgc gttttcccat ggccatcgag
    23161 aggattcaca cgaaaggagc cgctccatga agacgacgcc ttccctttgg gaacgtggtg
    23221 ccgcaattgc ggctttgtgt tgcttctccg ggatcctttt ctcgtttgcc ggcacggccg
    23281 ccgggaccac ccccccggag gcggccgttc agactccctc ggaggcagcc ggtcccccgg
    23341 aggaagcctc tcctcccgta cccaaagaga ccaagggacc caaggggacc gaagaagccc
    23401 tgcagagtag ggtcaaggaa gccctcctgc gggaatcctt ctcctatccc accggcgatc
    23461 tgttggatcc tttcattcct ttcatttccg ccgtggaaac cccgcccccc cagatcgcga
    23521 gcaaagagga aaccgacacc gagctgcctc ccgagcaaca gaggcccctc actccgttgc
    23581 agaagatgac cctggccgaa atcgagaagg ggctccgggc gatcacctgg ggggacctgg
    23641 gccgcaaagc cattatcgaa gacgcggccg gaaagggcta tatcgtcggc atcgggacgc
    23701 ccgtggggga caagaacggc gtggtgaccc agatcttcaa cgatcgcctg gtcattcagc
    23761 aggaagtttg ggatcggcag tccaagaaga tggtggccgt gaattccgtg gtgagaatca
    23821 agaaggccaa atgagccggg accggcggga atcgcccgtt atttccaacg gaaaaacgat
    23881 aggaggacca tcaccgcacc ggtgctgatg gcggtgtccg caacgttgaa cgcgggccaa
    23941 tggtaggtgc ccacgtagaa atccaggaaa tcgaccactt cccccaacca aacccgatcc
    24001 acgagatttc ccgccgctcc tcccaggatc aacgaatagg ccgcccgcac ccaccggtcc
    24061 cggggggaaa gcttccgata cgcatagaac aagatcccca tgacgaggag agtcagtccc
    24121 accaggaaga ccaggcgcag ggaggagtgg gccccggcca ggagactgaa agccgctccc
    24181 gtatttcgaa cgtggaccag gttgaaaagg cccggcacga tttcccgcat ggtgtgaagg
    24241 ggcatccact cccggaccat ggctttggtg agctgatcca ggagaacgat tccacccatc
    24301 accggcacca gtaacttcat gagccgttgt ccttccgctt tcccgcattg cctgtcccac
    24361 ggccgctcta tcccgtcaga accccgtaac accgctcgca caggctcggg tgatcgaaaa
    24421 actcccctac cctttccgaa tggacccagc atcgctcgca cttggcgccg ggggcaggtt
    24481 tcacggcaat cttcagtccc tcgacctcct gtccctctac tgcgtcctcg aggtcttccc
    24541 ctgcgaaaac cacccgggat acgatgagaa cggagcgcat caggccctcc ccgccgcgca
    24601 acatctcttc ataggaggca ggaagcctca aggagacctg ggcatcgagg gcgtggccga
    24661 tcttcttggc ctggcgagcc gcttccaacg cccgggacac atccgcccgc aaagccagga
    24721 tcttttgcca ccgctcgttg agcacctcgt ttcggtactc ggctttctcg tcgggaagag
    24781 gctccatgtg gacggattcc cccgaatgac ccggaaggtg ccgccaggcc tcttcggctg
    24841 tgaaggaaag aatgggcgcc atgagccgca ggagggcgga tagaatctcg tagagcaccg
    24901 tttgagccga ccttcgagcc ttgctgccgg cagcggacgt atacaggcgg tccttgagta
    24961 tatccaggta aaaggcgctc aggtccacca cgcagtagtt gtggatcgaa tggtacaccc
    25021 ggtgaaattc gaagcggtcg taggcgtttc gaacccgctg caccaggtcc tggagctggt
    25081 ggagggcaaa ctgatccaac tcctccatct cctcgtacgg gagggcatcg gaagctcgat
    25141 cgaaatcgta gaggtttccc aggatgaacc gacaggtatt tcggattcgt cggtaggcct
    25201 cgctcagtcg cttcagaata tcgggcgaaa tccggatgtc gtcccggtaa tcctccgccg
    25261 aaacccacag cctcaggacc tccgctccgt actggcggat gatttcctcg ggggcgatga
    25321 cgttgcccag ggacttggac atcttgtacc cctgaccgtc cactacgaat ccgtgggtga
    25381 gcacggtctt gtagggagcc gcgttccggg tccccaccgc agccagcagg gaggaatgaa
    25441 accatccccg gtgctgatcg ctcccctcga gatagaggtc cgccggcgac cgcagataag
    25501 gccgagcctc gaggaccccg gcataggaca ctcccgagtc aaaccagaca tccaggatgt
    25561 ccatctcctt tttgagccgc ctgcctccgc acccggtgca cttggcgttt tcgggcagga
    25621 gctcttccgc ggatcgctcg aaccagcagt ccgctccttc tttctcgaag agcgccgaca
    25681 cgtggtcgaa gacttctggg gcggccagca ccccgccgca atcttcgcag gtgaaaacga
    25741 tgatgggcac cccccaggat cgctggcggg agatgcacca atcgggtcga ttggcaatca
    25801 tgttgtggat gcgctccctg ccccacgacg ggacccactg cactcgatcg atccattcca
    25861 gggccttttg gcgcaggtcg ttcttctcca tggaaatgaa ccactgctcc gtggctcgaa
    25921 aaatgacggg tttcttgcag cgccagcaac acgggtagct gtgggtgatc cgagtctcca
    25981 ggagcaggtt ccccgtttcc ttgagcttgg caaccaccga gggattggcg tcgaagacga
    26041 actgcccggc aaagagcggc acctcgggag tgaaggcccc gccgtcgtcc acgggggagt
    26101 agacctcgag cccgtattcc agggccatat cgtagtcttc ccgaccgtga ccgggggccg
    26161 tgtgcacgca cccggtaccg gcatccaggg tgacgtgggt ccccaggatg aggaccgaat
    26221 cgcgatccag gaacgggtga cgacatcgga gcccgcgaag ctcgtcggcc cggaaggtcc
    26281 tcaaaacccg gtattccccg acgttgagga tcttcatgca ggattcgagg aggccttcgg
    26341 ccaggatcca cgcttcatcg cccacttcca ccgccacgta ctcgaaatcc gggtgaaggg
    26401 tgacggccag gtttgcggga atggtccagg gggtggtggt ccagatgaga accgatgcgg
    26461 ccctgcccgc cagttcgggg aatgcgtccc ggccttcggc tcgcaggggg aacttcacgt
    26521 agatcgaggg cgaaacgtgg tcgtggtatt ccacttccgc ttcggccagg gccgttcggt
    26581 ccaaggcaca ccagtagatg ggcttcttgc tgcggatcac gctgccgttg acgaaaaacc
    26641 gccccagttc ccgggcgatg acggcttcat aatcatagga catggtcaaa tacggatggt
    26701 tccattcccc caggaccccc agccgcttga actcctcccg ctgaatgtcg atgaacttct
    26761 cggcgtatcg gcgacattcc cggcgaatct gcacctgagt catggatttc ttccggggcc
    26821 ccagttcctt gtccacctgg tgttcgatgg gaaggccgtg acagtcccat ccgggaacgt
    26881 agacggcatc gtagccgctc atctggcggg ctttgacgat catgtccttg aggatcttgt
    26941 tgagagcggt gcccaggtga atatggccgt tggcgtaggg aggaccatca tggaggatgt
    27001 aaggaggacg cccttgaccc tgctccctca gtttttcata aagccccatg gtttcccatt
    27061 ggcgaaggat ttccggctcg agcttgggaa ggttcgcctt catgggaaag gccgtttgag
    27121 gcaggttcag cgtgttctta tagtccatgg tcccttctcc ctggcaaaaa tggtgagctc
    27181 ctgctgctcc ggcgggtccc cccggagtcc tcggtcggag cggaccccgt gagaaccgcc
    27241 ggaacgggct gagagcacgg ggcgcatccc ccgaacccag ctcgagggaa tcctttccgg
    27301 acaagctcct cgaggggccc acggggcgat gggcgaaacc gatcttttgg gccccgcctc
    27361 ccgggttccc ggagaggttt ttccttaaca caccctccag acaagtcaag tgcttacgcg
    27421 gcagggcccc ccccggggac ctcgtcccgc ctcccgttgc ccgcagttcc cggtgccggg
    27481 tacggcttcc ccggtgagcc tttgcaccca tggcgggtcg gttacggaga actttccgca
    27541 gggagcgcaa aatggaaaaa ccctctcccc ccgagggtat cgggaagaga gggtccgttg
    27601 cccgttgacg acggaagatg cgctcgagga ggcgccgacg gctccgtggt catggagaaa
    27661 atgcgaaacc ccggaaatcc gcctttttct tccgacgaaa gaaaggtctc tagtacatgc
    27721 ctccgccgtg gggcattccg cccatgccgc cgggcacggc aggttccttc tccttgggga
    27781 tctcggaaac catgcattcg gtggtgagca tcagggcgga cacgctggcg gcattctgca
    27841 gcgccgaccg ggtcactttg gtgggatcga tgaccccggc ttcgatgagg tcctcgaatt
    27901 ggttggtttc ggcattgaaa ccgaaggaac cggtttgacc cttgagcttc tcgacgacca
    27961 cggagccttc ttcgccggcg ttctgggcga tctggcgagc gggctcttcc agggcgcgcc
    28021 ggacgatgtt gaggcccagc ttctcgtccc cttccaggga aagggcctgc accgccggga
    28081 gacagcgcag gtaggccact ccgccgccgg gaacgatgcc ttcttccacg gccgcccggg
    28141 ttgcattgag ggcgtcttcc acccgggcct tcttttcctt catttccgtc tcggtggcgg
    28201 ccccaacact gatcaccgcg acgccgccca ccagttttgc cagccgttcc tggagctttt
    28261 cacgatcata gtcgctggtg gtttcctcga tctgggtgcg gatctggcga actcttccct
    28321 cgagggactt gcggtctccg gcaccgtcca cgatggtggt gttgtccttg tcgacacgga
    28381 cggtcttggc cttgcccagg tcctggagtc ccacgttctc gagcttgatg cccttttcct
    28441 cgctgatgac ctggccgccc gtgaggatgg cgatgtcatc gagcatggcc ttgcgtcgat
    28501 ccccaaagcc cggggccttg acggcacaaa cctggagggt cccgcggagc ttgttcacca
    28561 ccagggttgc cagggcttct ccttccacat cctcggcgat gatcagcagg ggccttccca
    28621 tcttggcgat ttgttcgagg acggggagca agtctttcat gccgctgatt ttcttctcgt
    28681 tgatgaggat gaggggctcg ttgagaagga cttccatttt ctcggggtcg gtgacgaaat
    28741 agggggaaat gtagcctcgg tcgaactgca tgccttccac cacttccagg gtggtctcca
    28801 tggacttggc ttcttccacg gtgatgaccc cttctttgcc caccttgttc atggcttcgg
    28861 cgatgatgtt gccgatggtg gaatcgttgt tggcggaaat ggtgccgacc tgggcgatct
    28921 ctttctgatc cttggtgggc ttgctgatct tcttcaactc gtcgaccacc acctggacgg
    28981 tcttttcgat cccccgcttg agggccatgg ggttagcccc ggcggccacg agcttcgagc
    29041 cttcgtagta gatggactgg gcgagaatgg tggccgtggt ggtgccgtcc ccggcgacgt
    29101 cgctggtctt gctggccacc tctttgacca tctgggcacc catgttctcg aacttgtctt
    29161 ccagttcgat ttccttggct acggtcacgc cgtccttggt cacggtggga ccgccgaagc
    29221 tcttgtcgat gaccacgttg cgacccttgg gccccaaggt gacctttacg gcatcggcaa
    29281 gagtattcac gcccttgagc agggattctc gcgctttgac atcatagatc agttgttttg
    29341 ccatgaaccg ttcctccgta gagttttgtt gggatcagaa gacgggatga aagaatcgcc
    29401 gggtctattc aatgaccgcc aggatgtcat cttcccgcat gatgaggagc tcttcgcctt
    29461 ccaccttgat ctcgttgccg gcgtatttgc tgaagagcac acgatcgccc gctttgacgt
    29521 ccagtggtct tcggctgccg tcctccatca gctttccgtt gccgacggcg aggacttctc
    29581 cctgcacggg tttttccttg gcggtgtcgg ggatgatgat tcccccggcc gtcttctgtt
    29641 cttcctcggt tcgcctgacc acaactcgat cattgagcgg tttcagtttc atggattcgg
    29701 ctcctttcca attcgtcagt acaccagttg ggatttgaac gaaggcgggg acccggcttc
    29761 ttccgggagg gccccttttg ttcggctctt caagccgatt gcgagggcac acaccgccct
    29821 cttctattag cactcagact tagtgagtgc ttataatcat tcgtggattg ctttgcaagg
    29881 gggaagaagt ggggatgggg aaaaaaagtg aaaaggtggt gcccccctca tcgtggtgag
    29941 tcacctcgat acgcccgccg gcattttcca ccagttgata gacgatgctg agtcccaggc
    30001 cggttccgct ctgcttggtg gtgaaaaacg gctcgaagac ccggtcccga atctccgccg
    30061 aaatcccttg cccccggtct cgaatatcaa tgcgggcctc gggtaccggg gaatctttcc
    30121 tcgagctccg gctcacccgg atggacaggt ctccgccgtc cggcatcgct tccagggcat
    30181 tcaccagtag attccagaga atctgttgaa aatgatgagg gtccagggac aaggtaggac
    30241 cgtcgtcaaa aacggtccgg attcgatgga ggacagaaac cctctctcgc gatctcaaca
    30301 aggccacgac ctcccggatc gcggaacaca ccgacaggtc ctcgatcctt ccgggctttc
    30361 tggacccctt tgccaaccac aagaaatcgt tgaccaggtc tttgatccgg aggatctctc
    30421 gatccacgat gtccatgagc ctcgattgga tcgaatccgc ctggatttcc ccccgcagca
    30481 tttggaagga cccgctcatg gccgccaacg ggttcttgat ttcatgggcg atttccgccg
    30541 cgatcttccc ggcaaaggcc agccgttcca tccgccgcac gtgatcttcc atggccttaa
    30601 gatgggttag gtcatgaaag atgacgatcc agccggagac ccccccgtcc ccttcctgga
    30661 gaaccgaaac cgtgtacccc agggtcaggt cctccccatc gggtcgacgg tagtggattt
    30721 cctttcttct cgtgctgcgt acggccgaag aaagggacga cgggacctcc cgggaccagt
    30781 cgacgggatt caccgaagaa aatacctctt ggaggggccg cccccgaagt tcttccgcct
    30841 tcaaacccag cgtctccagg gccgcccgat tccagaaaag caccgtgccc ttctcgtcga
    30901 tggtgaggag tccgctgctt atgctttcga caatgttgcg atgcagggcc tccagttgtc
    30961 gcaggtccga tttctgcttt tccacctgac gactcgaact cctcagctct tcggcgagat
    31021 atccgctgag ataggccacc aggtaaaacc ccgcaatgct catgagtatg gtgatgaaat
    31081 aggtgccgga gtccctcagg tgggtggtgt cggccacgat ctgcagggga gagatccagc
    31141 cgaagtattg aagatcgaga aggaatccgt aggaaaggct gcacatggag gcggtgagga
    31201 ggctcccccg acgttgcagc aggagggcgg cactgatgat gaccggcatg tagagaaacg
    31261 caaagaggct ctcgatccct cccgaaagaa agatcaggac cgtcaccgct tccacgtcga
    31321 aaaagagctg aatgtagccg aaccgttcca ggtgccggat gtgtttcaag ctgaccgcgg
    31381 ccacgatggt gaagaggaag atcaccatgg caaagaagta caggggctga atacgagcgg
    31441 ccaggaggtc cccctcccgg cgactctgca ccaccagggt gagcaccaga aagaagaccc
    31501 cgagcaggag gcggaagagg aggagcccct ggatcttgcg cttcatggag agctccgtgc
    31561 gggcggatgg gggctgtgca tcccattcgg gagacacggg gccctatcct cgacccacca
    31621 cgtccgcgat ctggaaaatg ggcaggtaca tggcaatgag cagaaatccg atggtgaccc
    31681 ccaaaaacac gatcatgagg ggctcgagca tggaggtgag ggcttccacg gcgatgtcca
    31741 cttcctcgtc gtagaagtcc gcgattttgc tcagcatctg gtccagggcg ccggtggcct
    31801 caccgatggc aatcatctgg gtgaccatgg gaggaaacac ggcggtttct tccagaggct
    31861 cggccacggt gcggccttcg gcgatggcgg cccgtgcctt gcgaatggcc cgttcgatga
    31921 ccgtatttcc cgaggtggcc gcaacgatgt ccaggccttc caggatgggc acgccgctcg
    31981 agagcatggt tcccagggtc cgggtgaatc gggccaccgc caccttgcga atgagaatgc
    32041 cgaagaccgg ggccttgagg aaaaggcggt ccgtgatgtc ccggcccttc tccgtcttgc
    32101 gcacccgctt gagcagaaaa atgagaacga tcaggcccag gaccatccaa tggaaatagg
    32161 ccttggtgaa atcgctgacg ttcatgacaa acagggtgag ggccggcagc tttgccccgg
    32221 catccttgaa aaggcctgcg aaaacgggga tcacgtaaat gaggatgacg gccacgacga
    32281 tgatggcgat ggccacgacg atgatggggt aagtcatcgc ccctttgacc ttcttcttga
    32341 ggttggccac tttttcgatg taagccgcca ggcggcccag gatggtgtcc aaaatgccgc
    32401 ccgcctcccc cgccgccacc aggttgacga aaagatcgtc aaaggccttg ggatgctttc
    32461 gaatggcatc ggacagggtc gccccttcct ccacgtccac cttcacctgt ttgacgatcc
    32521 ttcgaaaggt gggattctcc tgctgttccc ggaggatttc cagggcctgc accaggggta
    32581 gaccggcatc gatcatggtg gcaaactgcc gcacgaagat caccaggtcc cgttccttga
    32641 tctttgcctg caggaaagga aggtactcgc tgaggtcctt gggcttctgt tttaggcttg
    32701 tgatgttgat gcctttgcga gtcagaagtt gctgggcgat gccgggattg tccgcttcca
    32761 tttccccttt ttgcttcttc ccggcccggt ctgttccttt ccaaagataa ataggcatga
    32821 gtctctcctg ttggggcgga ggggtcggcc gccgtgacgc cttccccggc cactaatcct
    32881 tgacggaaca acggatcact tcctcgatgg aagtaattcc ggccttgagc ttgttgatgc
    32941 cgctgcgccg gagagtcacc attcccagcc gaaccgattc cttcttgatc tccgccgagg
    33001 aggcccccaa gagcaccagt tcccggatgg gatcgtgcat cggcatgact tcatacaggg
    33061 cgattctgcc ccggtatccc gtatgattgc atacgggaca gccctttccc aggtaacagg
    33121 caaaagaccc cagctcgtct tctcgaacct ccaggtccag caacgcttcc ggcggaacgt
    33181 tttcctcgat gcggcattcc gtgcaaaccc ggcgggccag ccgctgggcc accaccaggt
    33241 tgaccgcgga ggacaccagg aaggattcca cccccatgtt caggagccga ctgatggtgc
    33301 tgggggcatc attggtgtga agggtgctca ggaccaggtg accggtcaat gcggccttga
    33361 tcccgatttc cgcggtctcg aaatcccgga tttcccccac catgatgatg tccgggtcct
    33421 gcctcaaaaa cgagcgcaaa gccgaagcga aattgagccc gatggcatcc aggatctgca
    33481 cctggttgat cccgggaagg ttgaattcca cggggtcttc cgccgtggat atgttgtgac
    33541 tggtcttgtt cagctccgcc aaggcactgt acagagtggt cgtcttcccg ctccccgtgg
    33601 gaccggtgac cagtaccatg ccgtaggggg catgaatggc ggaacggaag gccttgtact
    33661 gggattcttc gaaacccaac ttggtcatgt ccacctggag gttggacttg tccaggagcc
    33721 gaaggaccac cttttctcca aacagggtcg ggagaaccga cacccggaaa tccatctcct
    33781 tgcccttggc cttgagcttg atgcgcccgt cctggggaag cctccgttcg gcaatatcga
    33841 gccggctcat gatcttgatt cgcgaaatga tggcattgcg cagggccagg ggaggacgca
    33901 tgatctcgtg gagcattccg tcgatgcgaa aacgcacccg catcatcttt tcgtagggct
    33961 ccacgtggat gtcgcttgcc tgctttcgga tggcgtccat gaggatgagg ttcaccagtt
    34021 ttaccaccgg cgcctgttcc gccgcatcct cgaggaagga caggtccacg tcctcctggg
    34081 attccaccac atcgacgtcc acctcttcct tgagcatgcc cagcatgtct tcgatgtttt
    34141 catcggcccc gtaatgctga ccgatgatct tcttgatgga cgtctccgag gccacgtgga
    34201 cgtggatgtt ctttcgggtg aggaagcgga tgtcgtcgat gacgaacagg tttcccgggt
    34261 ccgccatggc cacgttcagg gtgttgccca tgatgccgaa gggaattgcc tggtattttt
    34321 gagcgacatc catgggaatc aggctgatga cggccttggg gaccgaaagt ttcgccaggt
    34381 ttacctcggg gacatggaac tggcgcgcca agaaccccac cagggtgtct tcgccgatca
    34441 tccccagcct gatgagcacg gccccgatgc gctccccggt ctggttgtgc tcttccaggg
    34501 cacgagccag ctggtcgttg tcgagcaacc cttcggaaac caacaattcg ccgatcttct
    34561 tggacacgaa cgcttaaccc ttctctgaac gtcgccgcct gttcggattc tcaggtttct
    34621 ccggcatcga cccttccccg aaacgctctc ccattatcgc atcgtgggtt ttgatgcgcc
    34681 acgagatttt gggccgatac ggacgatcaa aaggcttgac agggccgaat tcctggttat
    34741 tattcgctgc aattttcctg gaggatggac caaaatatca gattcggagg tatttgtcat
    34801 gatttgtgcc actgtgaagg ccggttcgga atgcgccttc atgaccaaaa gcggttgcgg
    34861 gttcaacggg ggctcctgtc atcccattgt ggatcagtgt gaaggttgtc agcgagtggt
    34921 ggatttggcc tcgggaagat actgcaacag ttaccccaat cctgcggcca agtggaggac
    34981 cagcgtgtgc aacttcgcca gccacgccaa ggcgtccacc caggcgcaac agcaagcgaa
    35041 aatcaacccc ctcaaggcct cgaagcgaag caccaagcga aagtaacgga tcattgggag
    35101 aggtgggagt gatccgtccg gccggccgga acgagtcccc ctcgatgtca ttgctctcac
    35161 ttcccaatga caccctttca ggggcacctc ttttctatcg gaatcccccg ggaaaatctt
    35221 catcgaatcc tgcctgcgga aatcggcgca aaccctcgcc ccgagatgcc ttcggctttg
    35281 tatgtgcttt taccgccggg acgggttatg gtattctcac tcaaggcagg agatttcaac
    35341 ccatcggcct gtgtttttgg ccgggaagtg tcgcaaggat acggggacga tgccaagaaa
    35401 ggattccaac acagtggagc gcacacttat tttgacgggc actcaggcag ccgctctggc
    35461 tgccaggctt tctcgcgttc aggtgatcac cgcctatccg atcacgccgc aatccaagat
    35521 ccccgagatc ttatccgagt acgtggagaa gggagagctc caggccgagt tcgtgcgggt
    35581 ggagagcgaa cattccgcca tgggggtctg tatttccgcc tccctggtgg gggccagggt
    35641 gtttacggcc accgccgcca acgggttggc ctatatgcac gagcagctgc actgggccgc
    35701 cggggcgcgc cttcccatcg tgatgccggt caccaaccgc ggcctggggg ccccgtggac
    35761 catcctgaac gacatgcagg ataccctctc ccagcgggat acgggttgga ttcaattcca
    35821 ctgcatgaac aaccaggagg tgttggacaa gatcatcctg gcctaccggg ttgccgaaca
    35881 gatcatggtt cccatcatgg tctgttttga cggcttccgg ctttcccaca ccatgatgcc
    35941 cgtctccgtt cccccccagg accgggtgga tgccttcctg ccgccctacc gcaccccgta
    36001 cgaactcagt cctcacaatc ccgtaaacat caaccccgtg gtcatgtcgg accccttgcc
    36061 cgacagccgg ggaaggcttt gtcccagcta catgggcttt cgttttcggc tgcaggagga
    36121 gctcgagtcg gcacttgccg tgatcgcgga tgcagggcgc cggttcgggg agttcaccgg
    36181 ccgtttttac gacgacgtgc tgtatcgcta ccgggcgcag gatgcggacg ttcttttcgt
    36241 caccatgggc tctctttcca gtgaagcgac cgacgccgcg gacctgctcc gggaagaggg
    36301 cgtgaaggcg ggagtggtcg gcctgggggt ctatcgaccc tttcccgccc gggaattggc
    36361 cgaggccgtg caaaacgcaa agggaatcgc cgtcctggaa aaggggatca gttacggcta
    36421 cgaaggagcc ctggccacgg agctcaaggc ggcccttttt agccacaacg gccgtcgccc
    36481 cacccttcac aactacatcc tgggtctggg gggaaaagac gttaagccct cggacctgac
    36541 gggaccggcc agggaagtca tggaatccgc tcacaaaggg attcggcagg aaaaaccccg
    36601 ctggctgggg tacggaatct gatatttcgc caagagaaag gggccaggca ggaactatgt
    36661 cgacgacgat catggaattc cccgaccagg aatatattct ccccggcacc cgcacctgtg
    36721 ccggttgcgg cttgcccatt gcgtatcgct atatcctcaa ggccttaggc ccaaaaacca
    36781 tcgtaactca ccccgcatgc tgcctcacca ttttgcacgg gatctacccc aggacccccg
    36841 tcgccgtcaa tgcggtgaac aacacctttg ccagcacggc ggcttccgct tcgggattgg
    36901 ctgcagggct caaggcgacg ggccgggagg attacaccgt ccttgccatg gccggtgacg
    36961 gcgggacctt cgatatgggc atccaggccc tcagcggggc cgccgagagg gaaacggatt
    37021 tcatctacgt atgctacgac aacgaagcct acatgaacac cggcgtccag cgatccagcg
    37081 ccaccccttc gggggctctc accaccacca ccgccgtggt gcccaaggaa caacccaaga
    37141 aggatttcat ccagatcatg gaggcgcatc atcccgccta tctcgccacc acctgttcct
    37201 cctttcctgg agacctctac aacaagttcg ccaaggccaa ggacatccgc gggacccgct
    37261 tcgtccatct catggtcccc tgcccgtccg gctggggatt tcccaccagg gacacggtga
    37321 aaatcggtag actggcggtg gaaaccgggg tggtcgtgct ctacgaagtg gagcgcggcg
    37381 ggcccattcg cctcacgggc aagagcctca gcatggcgcg aagcggcagg aagcgaccgg
    37441 tggaagaata tcttcggcct caaggtcggt tcaagaaaat gactcccgag cggatcgctc
    37501 ttttccagca ggaggtggac caacggtggg cggggctcct gcaacgggcg ggattctagt
    37561 tccagcaacg cgaaaaagaa cgctcttaac gggtcgacga gggtctcgtc ctcggggcgg
    37621 aggcccgaac gggaccccaa ggtgtgctac gagccccggc tttcctcggg aggacggagg
    37681 gggccccgtt ttcggttttc tactcccacc accgcaacaa ggcgcttccc caggtaaaac
    37741 ccgccccgaa agcagacatc accaagtagt cccccttgcg aacccggttt tcctccacca
    37801 ccgcctgata caggcacaaa gggatggttc ccgcggtggt gttggcatag cggtcgatgt
    37861 tgacgatcac cttgctcctg tcgatcttca tgcggttggc actggcttcg atgatgcgca
    37921 ggttggcctg gtgagggatg aaaagggcca catcgtctcc cgtcagccgg ttcctctcga
    37981 ggatctccgc agaaacattg gccatctcga tgaccgcgtg cctgaagacc tgcctgccct
    38041 cctggtacac gaaatgttcc ttgttcttga ccgtctcgca actggcgggc cgcttgctcc
    38101 cccccgcctt catgtgcagg aagtctcccc ccgccccgtc cacgtactgt acgaaatcca
    38161 ggaccccgta cccttcctcg gggcagggct cgagcagcgc agctcctgcg gcgtcgccga
    38221 acagcacaca ggtgttccga tcctcgtaat cgatgatggt gctcatgaca tcggatccga
    38281 tcaccaggac cttgtcgtac ctgcccgcct cgatcatctg ggcccccgtg gtgagggcaa
    38341 aaacaaaggc cgaacacccc gcggacaggt cgaagcccca ggcccgggaa gcgccgatct
    38401 ccttttgcac cagacaggca gtggccggaa agagcatgtc gggagtgacc gtccccacga
    38461 tgatgacatc gacctcttcg ggagaagccc ccgccctctc gagacattcc cgagcggcgc
    38521 gaacagccat ataggagctg ccgaggcccg gctccaggat ccttctttcg cggattcccg
    38581 tacgggtcac gatccactcg tcggtggtgt ccaccatgtc ctcaaactcc cggttcgtca
    38641 tgcgccgttc cggaagaaaa cggcccacgg aacgaacgta tgcgtttctc cgcggcgttc
    38701 ttcgatttgc cattacatga ttcctttata atatcccccg tcgatctgga tggacgcacc
    38761 cgtgatgtac ccggcctgct ccgaggcaag gaaagccacc aaggcaccga actcctccgg
    38821 ggtccccagt ctgcccatgg gcacctggga ctcccatccc cggatcacgg cctcgggggt
    38881 ggtgccgtcc ctttccgcca cgaaccgagc caggctc
//
LOCUS       DAOOVC010000023        37590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_710_length_37590_cov_6.801279, whole genome shotgun sequence.
ACCESSION   DAOOVC010000023 DAOOVC010000000
VERSION     DAOOVC010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 37590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37590
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_710_length_37590_cov_6.801279"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            484..1149
                     /gene="rhuM"
                     /locus_tag="PLM79_05045"
     CDS             484..1149
                     /gene="rhuM"
                     /locus_tag="PLM79_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOV85707.1"
                     /translation="MSPESVVRNFRITAADGKTYDTQHYNLDVVISVGYRVKSHRGTQ
                     LRIWAAQRLREYIVKRLFKSGSDRFRERWRRDYAVAGTQGLSLRHLYRAMAFLGEETR
                     DQARAPRCTKEVIEEALFLHNRHLFGSLDLVFFDTISITFEGAGGKTIGRRGFGAVGR
                     PQSMCRGLRQGLSGRRRCHATHHQGDLTAQMTSGSATRYSCARILLLSQKKFIDSVED
                     ESE"
     gene            complement(1325..2800)
                     /locus_tag="PLM79_05050"
     CDS             complement(1325..2800)
                     /locus_tag="PLM79_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOV85708.1"
                     /translation="MQRGKEPSSPPVPSYPSKLLVVGLLLPALVAGSLAPQPGQATAA
                     TSPGTESRSSSPSTPPSFTELAEKLAPSVVNIKITRVERAGFHGMPFQGSPFDDFFGP
                     FSGGGPRPPGPRRVEGAGSGVLIRSDGYILTNNHVVEKAREVTVTLSDQKEYKARVVG
                     RDPKTDLAVLKIEGKEPFPSAVMGDSDALKVGEWVLAVGNPFGLGRTVTSGIVSAKGR
                     VIGAGPYDDFIQTDASINPGNSGGPLFNLQGELVGINTAIIADGQGIGFAIPVNMAKT
                     LVPQLISHGEATRGHLGVTIQPVTEDLAAALNLPDPKGALVGDVTRGSPADKAGIKRG
                     DVILEYDGKPVKDSRQLPAMVAATPVGEKATVGVLRNGKELTLEAKIAGSPSQTARET
                     DAEEPAQGKWGLMLRELTPDLAQRHGIVGDRGVLVVQVKPGSPAEEASIRPRDILLEV
                     DRHPVSSPEDVKERLNGGKSPDRHLVLIQRGENTLFAVLKG"
     gene            complement(3055..4455)
                     /locus_tag="PLM79_05055"
     CDS             complement(3055..4455)
                     /locus_tag="PLM79_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85709.1"
                     /translation="MPVRSIRARLTFWYALTFTAALLVLGAAAYGLLSYSLSRQIDAS
                     LNGVAQALTEKSRGGAAGLVPSEVDEVFRRFFGFSPWDPYLQFLDPSGRRDPREPPAR
                     RPRLPLSSRALENASMGLPTYETIEGLESGPIRLLTLPRTEGGRVAGLIQVGTSLQGL
                     RETRFRFMLIMAGLLPLGLLLAAGGGRLLARRALSPVDRMTEAARHISAEHLTEQLEQ
                     TGTGDELDRLAATLNDMLKRLDASFRQIRQFSADASHELQTPLTILKGEVEVALRAPR
                     SAEEYVATLESALEEIDRIARLVDGLLTVSRAQAGVLRMDTRPVDLARLLEGELQRLG
                     VLAHGRSVELVSGFIEPLTIPGDAARLQQLFANLLENGLKYTPAGGTVTLSLARNGNW
                     AVTEISDTGIGIDPREREKIFQPFYRTTQARNLGEKGVGLGLSIVQSIAQAHGGKVEV
                     LGHPGRGSTFKVFLPV"
     gene            complement(4459..5130)
                     /locus_tag="PLM79_05060"
     CDS             complement(4459..5130)
                     /locus_tag="PLM79_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV85710.1"
                     /translation="MRLLVVEDEKKVASFIQKGLTEEGYAVDVAHDGQTGLEMAGDGI
                     HDLIVLDIQLPKMDGLEVLRELRRRKIRIPVLLLTVRAAIEDKVLGLDSGADDYLTKP
                     FAFQELVARVRAILRRGTEARCPVLLAGNLTLNPATRTVHRGPRKIDLTPKEFALLDY
                     FMRNSGRVLTRTMIAEHVWDYDFDTTTNVIDVYVNYLRKKIDLPGEPKLLHTVRGVGY
                     MLKPE"
     gene            complement(5160..5669)
                     /locus_tag="PLM79_05065"
     CDS             complement(5160..5669)
                     /locus_tag="PLM79_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV85711.1"
                     /translation="MSRPGPPFPSSSAVLPTCALSRLNAREAFEASRTLVEMDPWKTL
                     GYRPEWLLEYLLREDSCLFRYGMFHEERVCGVLCVRHPWLLGPYIEILAVFPDRQGQG
                     WGREVVRWIEDRARPTSRNLWVTVSSFNQKARDFYLRTGFTEIGVLSNLVRKGFDEVL
                     LRKELRPAT"
     gene            complement(5666..6421)
                     /locus_tag="PLM79_05070"
     CDS             complement(5666..6421)
                     /locus_tag="PLM79_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOV85712.1"
                     /translation="MNSASSRSHILKVLVFLLVFPIPAAGEVITRLPTREPRIALTFD
                     ACEMGQPHYLDAAVLDTLLSEQIPFTLFVSGKFARRNREELRRIAARDFVEVENHSLS
                     HHHHMERLAPGRVREEVLVAGQLIEEITGKKPQFFRFPAGNYDAKTLRTVEDLGYRVV
                     HWTFASGDPDIHLTPERLTRRVLSRSTNGSILIFHINGRGYATGRALPQILDGLRSRG
                     YRFVTLKEALDPHGNRSTPPGAGAFSPTAGEVP"
     gene            7024..8643
                     /locus_tag="PLM79_05075"
     CDS             7024..8643
                     /locus_tag="PLM79_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOV85713.1"
                     /translation="MTAFLTRGSLHLERIAGILLLAVFAVSCAKVGPDFVRPGAQVSD
                     QWIESNDERVRTEIEDHRQWWTSFQDPVLDRLIEIAYRENLTLRTAGVRVLQARAELG
                     IVVGRLYPQSQSLSGSWDRSRSSERSAGAPQDPHQLTSSAYFTTSQAQVGGAAAWELD
                     FWGKFRRAVESADASLLAAVADYDNALVTLTAEVATSYIQLRTYEERLKIAHENAVIQ
                     REGLRLAEAKFRGGATTQRDVEQAKTLLAGTEATIPALQILVGQAEHSLSLLLGKVQG
                     QLDEILGESRGIPSPPLEVAVGIPAELLRRRPDIRSAELQAAAQCAQIGVAKADLYPH
                     FSLQGSFGFLSTDVGRFKLEDMFLWKSRTGSLGPTFQWDVLNYGRLTNNVRVQDALFQ
                     QAILTYQQAVLRAQKEVEDALIGFLRAQEQAKLLKESTEAATRSLKLAMAQYREGMTD
                     FTTVLTAQQALLEQQNNWVSTLGDIGLNLVSLYRALGGGWQLREGQDFVPAPTKAAMS
                     ERTDWGNLLDPKALPSSTQDRKEPAIRRPDW"
     gene            8709..9863
                     /locus_tag="PLM79_05080"
     CDS             8709..9863
                     /locus_tag="PLM79_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV85714.1"
                     /translation="MIAIWGLAAAILGLSLGCGEKQTFVPPPPPKVTVRQPIRQAVTI
                     FRDFTGNTQAINTVQLRARVEGYLEKVLFQDGAKVKQGQLLFQIQADTYEAKLKQAEA
                     EVLSQKASLEHAEREYARFSQLFKENAAAATDVSKWLFERDSARAKLLSAQARMELAK
                     LDLSYTKVTAPFDGRIDRRLKDPGNLVGSGEKTVLAEINQIHPIYVYFTISERDLLEF
                     LEIRRAKSQQKQKPDWSLYMGLANQQGFPHIGELDFGSITLDAKTGTLLLRGVFPNPE
                     GEIWAGMYARIRAPVATEPDSLQVPETAVASDQQGRYVLVVNDKNVVERREVQVGPTW
                     NQMMVVKKGLTGNEWVVVNGLLRAIPGHEVTPSREEGPAKTSSPSESQGS"
     gene            9982..13140
                     /locus_tag="PLM79_05085"
     CDS             9982..13140
                     /locus_tag="PLM79_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV85715.1"
                     /translation="MISDFFIERPIFANVIAIVTIILGGVCLFTLPVAQYPDIVPPTI
                     QVSTSYPGASANVVAQTVGVPIEQAVNGVERSIYMSSTSASDGSYTLTISFSVGTDLN
                     TALTLVQNYVQGALAQLPTSTQSQGINVKKVSTNILCGVSLYSEDDRYDETFLSNYAI
                     INLENPLARLPGVGQARVTGAGKYSMRIWLDPTKLQYYNLTVLDVQNAISQQNVQVIA
                     GKLGAPPVPDEQAFQYTINALGRLSEVEEFENIIVKTIRGEETAQIVRVRDLARVELS
                     QQIYANFAEVTGHKAGNIFIYALPDANAIKVSEAVKEAMGEMSKEFPEGLKYQILFDT
                     TDFVKEAISKVYETLIEAGILVLIVIMVFLQNFRAMLVPATTVPVTIIGAFAAMAMLG
                     FTINLMTLFALILAIGIVVDDAIVIVENAAHYVEKGIPPKQAASKAMKELTGPVIGIT
                     LVLTAVFLPAAFLPGITGQLFRQFALVIAATAVISAVNALTLKPAQCALYLRKQTKEL
                     NVFYRGFNRVYSVIEGGYTAVVRWMVHRYQLMVVVFFVVIGIAGWMFAQQPTGFLPTE
                     DQGYGMVFAKLPDGASQPRVREVSKKVDGILKKTPGIKGWVTIGGYSVLDGANAPNVF
                     TKFLIYDDWGKRGTELSQEKIVANLRRSLYSVEEAFLGVLIPPPISGLGQSGGFQMMV
                     EDRGSLGLEELEKVVMELLRAANAQPGLRNVATTFTASTPQLFLDIDRTKAESLNVPL
                     NDVFSTLQAYLGSAYVNLFNKYNQVFQVYIQADAPYRLKTEDIMGLYVRNQDGNMVPL
                     GTLMDARMIESSEIVNMYQLYPAAAVFGGAAPGFSSGQALDLMEQVARNTLPRGVNFE
                     WTATAFQEKQVGSMAYFIYALSLTLVFLVLAAQYESWTDPAAVILVVPMALVGVLLAL
                     ILRGFDNNLYTQVGLVLMIALASKNAILVVEYARELRAEGMPLMDAAVEATRRRFRPI
                     VMTSFAFILGVVPLLGARGAGAASQQAIGTVVFGGMLSSTILAIPFIPVFYVLMQKLS
                     GRFGKRKAEEHTEAVEPAPEAS"
     gene            13262..13465
                     /locus_tag="PLM79_05090"
     CDS             13262..13465
                     /locus_tag="PLM79_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85716.1"
                     /translation="MNEKLRQFYEDSKALITYLLEEHVFDKMADCGCGGVDAYRSERF
                     DELIRKAAKSLGEFEREMSPPGP"
     gene            complement(13804..15672)
                     /locus_tag="PLM79_05095"
     CDS             complement(13804..15672)
                     /locus_tag="PLM79_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85717.1"
                     /translation="MNPPLFRWILAACLVSLLFGLPVSVAFGADPVTVITVESTEDNY
                     NDGNSKTCQNATPCTLRRAVNQAYSLGDGDRPVLINFDIPTTDSGYNDLLQAWKIELT
                     GTTLNPLRDLNGKVTVDGSTQPGGRSGAPKIILDGKKAKNYGFVMRYDANAVIGLAMQ
                     NFKTAHISVAGHNNTVSDNWFGLSDDGTLLSAGDEETYEGGSAVLVSSGCDSNVISRN
                     MFAGFWGVACSINGSHNQFTGNWVGTRANGTIPLPSGFTKHPCSSGGWVGGSGISVAG
                     SNNQVGGPNAGDGNVFAGLFLDLSETSTQGPAIKVYPGTNHLIQNNVVGLDSAGKAVG
                     VCGRGLDLGSGPDELQVLNNTFVETGLSAVLMNSSVESNALRSNVIKRKTQWPGAQGD
                     NGFAEDAIAYGPMVPAALKGFKPAAVTKIDGTTVKGTSGAGSVCAECIIELFLDDLDS
                     VKECLTPLQFVTADASGNWTANLAAALQPTQGLRTMSTVPDSFTITGLKANTTSNLSQ
                     IYSKLPKVSVTAPDNVATEAGATTGTFKFARDLAATSVTIYYKVAGSAVAGVDYTALP
                     GKVTMAAGVKTALVKVKPKNDTKKEVNETVKVTLVDKPAYDLGTPTQATVTIKDND"
     gene            16239..17351
                     /locus_tag="PLM79_05100"
     CDS             16239..17351
                     /locus_tag="PLM79_05100"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOV85718.1"
                     /translation="MKLPSLTIGELVTKIPIVQGGMGVGISLSGLSAAVANEGGIGVI
                     ATAMIGLNESDLYSNYLEANIRALRKEIRKAREKTKGILGVNIMVALTNFADMVRTSI
                     EEGIDLIFSGAGLPMDLPKYLKEKSKTRLVPIVSSAKAAQILCKRWFSRFKRLPDAFV
                     VEGPMAGGHLGFRAEQLDHPNYALEKLIPEVVQAVEPFEKEHRTKIPIIAAGGIYTGE
                     DILKFLELGASGVQMGTRFVATHECDADIRFKEAYVKAGVKDLVIIKSPVGLPGRAIR
                     NEFVEEMERGEKKPFKCPYHCITTCRCKDSPYCIALALANAKKGNLANGFAFAGGNAY
                     RVDRIMSVKELISSLIEEYDQASCPLSSQRSSGSMA"
     gene            17617..19788
                     /locus_tag="PLM79_05105"
     CDS             17617..19788
                     /locus_tag="PLM79_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85719.1"
                     /translation="MSTEPSSNDISPANKWRFSRVGGFDQVSLQSGNDLLNLDQLDPK
                     LWAALSCPTHGLEIDARTLELIDYDGDGRIRVPELLEAVKWATSLLKNPEDLLKGEDS
                     LPLSSLDDSTPAGKELLSSARQILTNLGKPESTTISLEDTGDTVKIFAKTRFNGDGII
                     TRDSSEEEAVKAVIGNVLECIEPETDRSGSPGVSREKVDLFFAETQKFADWWKKGEED
                     PHSDALRLFYSDGGAVAAFQAVRAKIDDFFLRCRLAQFDPSAAEALNPSVEDYRLLTS
                     KELSRSSESLGSLPLAHIRTGASLPLTEGLNPAWTARIESFRSRVAAPLLGEKTSLTD
                     TDWEVLSEKFRAYESWVGSEEGTTVEKLGIDRIREILSGGYREAILSLIEQDEALEPE
                     ANAIQSVDKLLRYYRDLFRLLNNFVCFHDFYTPGRKAIFQAGSLYMDGRSCDLCVRVT
                     DVAKHSTLATLSHTYLAYCECTRNGEKMQIAAALTQGDGENLRPGRNGVFFDRQGRDW
                     DATIVKIVEHPISIQQAFWSPYRRLARMVAEQAEKFASAREKQVQASAAGAIEAGKKP
                     EAPKPGAEQPFDVAKFAGIFAAIGLAVGAIGTAIASVITSFLGLKWWQMPLAVVGIIL
                     MISGPSMILAYLNLRKRNLAPILDANGWAVNTKAMINIPFGSTLTNLAKLPEGAQRTL
                     LDPFAEKERPWKLYLWIAALVAGILVLLQSGYLGQWLRLLF"
     gene            19825..22677
                     /locus_tag="PLM79_05110"
     CDS             19825..22677
                     /locus_tag="PLM79_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85720.1"
                     /translation="MTTAPTLPDVSLARRELRELLRGSCILRPGPGQPLRTPDGEPAL
                     WLFYSWDATLTRRGMTLAAACLLEALSSFEASQLATVGYTGLPLLLGCILYGKDRYTG
                     LCVRDERKGHGSLRRIEGPGDRSRPVVLIDDSLVSGSSLCGAVEALEGEGFRVEGAVA
                     LVNFPWGGGIERLNALGYRVECIFDSVRDLQIDEPLPSTPHLDLLPDSWDHPPIPDGL
                     HPATAARMTMEHYLRTGKVPRPPGRMDSNYDGRGGVFVSLRERETSFRLARDGFYHFD
                     ARDSDPCRDVVLAAVATVRGAPELTRETLRTLKIAVSFLGPLRETIPGGLDFPRYGIV
                     VRSKGGERIGGALPNTEYFIGETEQYRHARSVNAQVGDFEPHDLFRHTVTKAVEPGAY
                     WLPFGSSANSQEDWTEKEEIGRRLTLRAREILAALPNGPQPGANPVPDDLIPAPLFSV
                     GVTLFHRGLLGCGLSWGDTLDRCLQQAVEIAYGDERFSGSDRGLQPNNWAVAVSILHD
                     REWLGEASLEEACTRMRRGLDSLSIEYRGGGDMLLPFVCVHENLSKEQMGEALLEKAG
                     NPGPPHHWAVYPTATWMEKDGKVSRVRFGFPDRLGDPYGPDRWREDAALLASYLLGHL
                     GSDGLPEYSYDPITGHRVKEAASPVRPLHALWALVEAGRVLECSQWCRRARRGLELCL
                     EHVREEKGGRWTLGLADYGKDLLADAVLLLALTAAGEDLRTDPKTEALASNLRSLVQP
                     DGRVTEMPEARGMDSDHDIFPAVVLLALGRYAENTGKPFPAQGIEAALDRYARRFRLV
                     HPWAMVGWHPQAWNALHAVSGNPAMAAFAGEVADWALAWQHEENGSFLCDLEPEGYSF
                     HTAFVAEGVADAGRLAARRGDLSGCDRYARSCSRALRFMNQLIIREEDTFCMANPAAS
                     LGGVRGAVFLSEVRIDYVSHTLLALVKALEAFPDFP"
     gene            22723..23355
                     /locus_tag="PLM79_05115"
     CDS             22723..23355
                     /locus_tag="PLM79_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85721.1"
                     /translation="MVKSPWKELVAAGAVSLLGVTAPAAEDAPARAAGASSVMTAQAP
                     AGQDSMTEPRGQVQAPERRVEARSKPPRYKPPDKRVEARPKTPQYKPPDTRVEARPKG
                     PVQPPPGRIVEARPTPPKDSKRVEMRYKTPDSGKKEPRHRREGPQSDAWRKSPGPGSS
                     KPQPRVEMRKKTPGDPGNRKIVEMRPKPPPTQGNQKRVEMRPRPPQNPEE"
     gene            complement(23549..25399)
                     /locus_tag="PLM79_05120"
     CDS             complement(23549..25399)
                     /locus_tag="PLM79_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="HOV85722.1"
                     /translation="MKVRPFPPVALVSMPTSSARYPCLQLGLLKPTLERAGFAVEVFS
                     LYAYFGSLIGWRLHEAVAEVPTCLAGEWIWAAHAFEENRDEDLYLETYGDQLDGLCRS
                     ARCTLEDLLRVRRELTGTFLDRCLDWTDWSRFGLVGFTVLFQQQMASLALARRIKERH
                     PDLPVILGGGVMEEDLAREILGNCPQVDFVHCGEADEVLPEMVRRLYGGESMEGLPGL
                     FGREGKGIFEGGRASNLEDMDRTPTPDYDEYFRAREEGGYAHYEGAAEPFVPIETSRG
                     CWWGERSHCTFCGQNRVGIRYRSKSAPRVLEMFEELASRYNILNLDAVDNVLDPDYVE
                     GVFGHLAEARADFKIVYAVRASLDRRQIARMRAGGLCCVQSGIESFSTHVLKLMRKGT
                     TARENLELLKWTTYHGIDNLYNILVGFPGETPEDYREQCDLFGLIGHLQPPYAMVKAR
                     PDRGSPMFVDPAAFGITRLNPEEVYRFLWPPDRFARDRVAYFFTPEIQGALPDEAFDE
                     IYRTVREWQERWASERRPSLTYAKTRSSIRIRDGRGGEVLEYRLAGREAELYESCGDS
                     RTLGEVERQFRGPAGWIVQFLEESLHRGLMVFLDGRYLSLALPENPYHGL"
     gene            complement(25552..28536)
                     /locus_tag="PLM79_05125"
     CDS             complement(25552..28536)
                     /locus_tag="PLM79_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85723.1"
                     /translation="MRCSGIEIIGDVPWGTHFCQFYETGLDLIETLVPYFREGLAGNE
                     FCMWITSAPLDVEEAKAAIRTGLPDADRCLREGRIEILDYSQWYIRSGKFSAEEVLRG
                     WVEKLEAAREKGFEGLRLSGNTFWLEADDWDDFTRYEETINNVIGRYPMMALCTYSLK
                     QCGASEIMDVVANHQFALIKRKGRWEIIESFRHKQMEDALRASEERYRGLVEMSPDAV
                     FVNRDNRIVFVNPEAVRLLGASDPEEVLGKSPYELFHPEYHPTLDERIRRLREGKAVP
                     LIEARIVQLDGALRNVEVAASPFEDREGSAIQVILRDITGRKEAEEELRESEARWRLL
                     AASMPNLVWTCTPDGECDYLSPQWVEYTGVPEAEQLGFDWLERVHPEDRDSLMAAWNG
                     AVASEQAFDVQFRIRRRDGVYRWFQTRAVPVRDRKGRIVKWYGSNTDIEDLKQAEEAL
                     RKSEHRYRNLFETMNEGFALHEVVCDEDGRPCDYRFLEINPGFEAQTGLRSSDLVGRT
                     VREVLPDTEPFWIERYGRVALTGEFDHFEHYSGSLGRHYEVRAFQTEPGHFAVLFYDI
                     TDRKRAEEALRASEARYRLLSDTANRLLGSDDPQGIVQELCREVMEHLDCQAFFNFLV
                     DPEVGRLHLNACAGIPEEEARKIEWLDYGVAVCGCVAQGLKPIVVEDIACTPDPRTEL
                     VQSYGIQAYACHPLIAQGRLLGTLSFGTRTRSRFSDEDLALMRTVTDQVAIAMERIQL
                     IDQLRKSRDVLEERVKERTVELERANEALRCLSSRLLFAHEAERKRVAGEIHDTIGAC
                     LSAVKFVTEGALQRMEKSGDGRVGPLKTVIPVIQESIEECRRIQMDLRPTILDDLGLL
                     ATFSWFCRRFQTIYSGIRIEQRFDIAEHEIPDSLKIVVYRVTQEAMNNIVKHSGAEQV
                     RLSFRKKDGRINLLIRDNGRGFDLEKALSTNSTDRGWGLLNMRERTELSGGTFDITAA
                     EGKGTTIRASWPLPGANPEEQP"
     gene            complement(29021..29620)
                     /locus_tag="PLM79_05130"
     CDS             complement(29021..29620)
                     /locus_tag="PLM79_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOV85724.1"
                     /translation="MNRRIGTVLGLIVALGFLGNVCLATEGEATEGKNPVVALKTSEG
                     TIRIELWPDKAPVTVRNFLRYVEEGFYSGTIFHRVIDDFMIQGGGMTPDMKPKSTHEP
                     IKNEAAPDLKNDRGTIAMARTGMVDSATCQFFINVKNNDSLNQRDKTPQGFGYAVFGK
                     VIEGMDVVDRIKKVPTTTVGPHRNVPAKPVLIQRVELVP"
     gene            complement(29867..29989)
                     /locus_tag="PLM79_05135"
     CDS             complement(29867..29989)
                     /locus_tag="PLM79_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85725.1"
                     /translation="MITMELTPEEAAVRRRVFLCRLSELRDGIGHTDSRNVGNC"
     gene            30391..32019
                     /locus_tag="PLM79_05140"
     CDS             30391..32019
                     /locus_tag="PLM79_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOV85726.1"
                     /translation="MGKSKRVPNHGRAFSTARFSPGASGKPYHGTLPGGKCFREKGRI
                     PGIARVLWLLAFLVVAQWEIPSCRAASRTPQSSLYPLSKIPLTKGRLASLPASRGPVH
                     MGIGEAEIQPVRLILDSPPEEEVQATLEPAGWEHALEVSLWKAEWVPGSEGNRGRVPD
                     ILVPVEFASPMVFSETDNLLFLKIKSLPNSAGFYELMLTLSGETTSLKSKVLVHVHNV
                     PISLPNLLIQGCLKEDKSFDLIEFLRDYGFTGICGVSTEWHVRRRPEGFKKLCDRILS
                     LGYKAIRIPNKTMYGHRRAFPYPDSADGYKQWLRDEVDYLNNLDDLLQDKKYRDVFSF
                     RCWDEPQADQYNQVYNTSRLIKENQPNLRLELTEPPDSGLSPYVDTWSLNIRYLDRPT
                     VLEAKTGGKKCILYANSLHGVNRKSYTYDMRAIGYLLWKFRLDGYYFWGLNDWEGDVQ
                     EMSTTGAPREYKRGILLYRLKDSGKIVPSLRLELFRDGLEDLSLLKYIAGKGEFKGLR
                     KEIHRKLKTFQMVDKQAVVPDMTKYHHRILSLIP"
     gene            complement(32151..32984)
                     /locus_tag="PLM79_05145"
     CDS             complement(32151..32984)
                     /locus_tag="PLM79_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOV85727.1"
                     /translation="MLFITFSREFASGGTEIAKRVAERLNCHYFDTEAINQAAEELGF
                     LQEVKKVDEKPPSFFQQLFSDKVSSGFFRLHSVIYELAKRGDAVFLGRGSSILLKDLN
                     CALRVRIVASREKRVENLLREGYRKEEADQILQKTDRERDAFIKFVYGVNWDDARYYD
                     LVLNMDKLSTDMAVETVVNLARSSDIRTAGIECQKAIEVLALTSRLQAAIVDAGPAFD
                     HGHSVSVSVTVPEPGKVVLSGWVANEAGKKKAEDIVRSIKGVETVENTIRIVRATRVK
                     D"
     gene            33809..34165
                     /locus_tag="PLM79_05150"
     CDS             33809..34165
                     /locus_tag="PLM79_05150"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HOV85728.1"
                     /translation="MKKSAFLLVVPALVFLFAKDALCVGAIAVDDEIGESEPGYGFVT
                     GAFSREEAKRRALDECGRSGNEDCRVVLWFESCGAYAASLTHYGVGWGSTKEQAKKRA
                     LDECGPGCVVKVAECE"
     gene            complement(34214..34522)
                     /locus_tag="PLM79_05155"
     CDS             complement(34214..34522)
                     /locus_tag="PLM79_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOV85729.1"
                     /translation="MNRNKKLTRFLVLVSVLVVLPWVAQAWGQAAAPQAPSAAVSPEA
                     PAAGAQSQPALTYHGNVKSRVFHKPGCQQYNCKNCTAVLKSREEAVKAGYKPCGMCKP
                     "
     gene            complement(34635..35432)
                     /locus_tag="PLM79_05160"
     CDS             complement(34635..35432)
                     /locus_tag="PLM79_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOV85730.1"
                     /translation="MDAKLKYRNRIVTDEDIALIQSLITEHPADSRRQLSKRLCQAWN
                     WVQANGELRDMVCRSLLLQLERAGQIQLPPKRCSPNNPLVRRKKPDRPSLDQTPIEVD
                     LCRLPELVIRQVRRLPQERLFNALVEHHHYLGYRQPVGEHLKHLILMGERPIAGENLE
                     DLLSRRLAQLPPPIRICDALSRNLPGTVKTILANCLIHGRRNFIDTIPAFPQTSRNAL
                     GRPDDGQRVVELREPPALLGGDRASALCKFGRGSRAVKAFGSGGNSH"
     gene            complement(35481..35819)
                     /gene="tnpB"
                     /locus_tag="PLM79_05165"
     CDS             complement(35481..35819)
                     /gene="tnpB"
                     /locus_tag="PLM79_05165"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HOV85731.1"
                     /translation="MIRIIPQMRIFLCVEPVDFRKGIDGLCRICRQVFGQDPFDGALF
                     VFCSRQRSALRILTYDGQGFWIAHKRLSIGRFHHWPERSHLDAHELQALIWNFKVPAR
                     KVRFFKKLPG"
     gene            complement(35816..36208)
                     /locus_tag="PLM79_05170"
     CDS             complement(35816..36208)
                     /locus_tag="PLM79_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85732.1"
                     /translation="MAIKYPLETQSRLQEVSAIFAHWRRNGKPRSRIPEELWQAAVDL
                     APCCSTHRIAKALRLNYTELKHRIGKRLSEEHPAQFVEIDMRQLWSTAPCVLELRSPT
                     GFELKIRNVERLQPQLRSLVSCFLGQRR"
     gene            complement(36242..>37590)
                     /locus_tag="PLM79_05175"
     CDS             complement(36242..>37590)
                     /locus_tag="PLM79_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85733.1"
                     /translation="LDAAYKEMSLTSGADLTQDLPPDLGSLMDRFLGEALHNEEEGWA
                     AGLKTGEIGPALGLYRERIRKHFAGRGPDALLKEVLGKRRIVERNDPCLLGTPPYKVA
                     AVGARYPALPDSLRHRAGLDLKDPDTGETLVSFSASLAELSGKRLGATFEPSTAADRQ
                     VLETARASSLPLYLVKVFPRVHLDGETVARGPDAPMGSSQVWAVTLWDPAGRTTATEY
                     FPASAGDELVFGINGAGISPRRVQHRRETVPEATPAENLYGVFLHYFMQCDLLDELWA
                     RSLGVRKERLPSVGLFSAPLSVGYFLGTPFSGNYPGRRMDVGRGLYAVGADRPEDRFI
                     FLLLTGIQDSYLEGAAWDAFFLRPPGTAVSAVQVLLDANEQQVPVHRITASNLEALSL
                     LTVDEELRETIRRGVLSGKTVLAPRGEISHGGWRGAAAVFRVFPARVRETAGGLGV"
BASE COUNT         7288 a        11630 c        11432 g         7240 t
ORIGIN      
        1 tttgtatgcc atatcaatat attacacatt cctatttatg gaacttcggt ttaaccggca
       61 aggtccgggt aaatatagct tctgaggaaa cagtacatga aaatagccac cattcttgat
      121 cagatagacc tgggcaccat tgccctgccc catttccagc gcggctttgt ctggaaccgc
      181 gatcaggtga agggacttat caattccctt ctaggccacc tcagcgcaga ccgagctggg
      241 catgctcaat gtggccaggg ccagcctgga tctgttgggg gataatccat gaccaacctt
      301 cccgccaacc cgcccgcccc gttccgagct cctcatctac cagaccgagg atggccgaac
      361 ccgtattcag gtcccgcttg aggatgaaac ggtatggctg acgcagaagc tcatggcaga
      421 attgttccaa aaggatgtgc ggaccatcaa tgaagctatc cgcaacatct ttgccaaggg
      481 gagttgtcgc cggaatcagt tgtccggaat ttccggataa ctgccgcgga cggcaaaaca
      541 tatgacaccc aacactacaa cctggatgtt gtcatttcgg tgggttaccg ggtcaaatcc
      601 catcgcggca ctcagctccg catctgggcc gcccagcggc tgcgggaata tattgtcaaa
      661 cggttgttca agagtggttc cgaccgcttc cgtgaacgat ggcgaagaga ctatgccgtt
      721 gccggcacac agggactgtc tctccgtcat ctctacaggg ccatggcttt cctgggagaa
      781 gagacccgag accaagcgcg tgccccccgc tgcaccaagg aagtcatcga ggaagccctg
      841 ttcctgcaca accgtcatct gttcggcagc ctcgatttgg tgttcttcga tacgatctcg
      901 atcaccttcg agggagctgg aggaaaaacg atcggccgga gggggttcgg cgcggttggc
      961 cgtccgcagt caatgtgccg gggtctgcgg caaggtcttt caggccgtcg ccgttgccat
     1021 gccacccacc atcagggaga tctaacggct caaatgacat ccggtagtgc cacacgctat
     1081 tcgtgtgctc gcatcttgct gttatcccag aagaagttta ttgatagcgt tgaagatgag
     1141 tccgagtgat acggcggtaa tcattgtttc cacgcacgga gggggtgaac cgatgagcga
     1201 tccggaaacg ctcagtgcat ggcgattgac cccgcgcacg tgggggtgaa ccggccgggg
     1261 tggagttttc cgtagggtgg aagaggcccc ggcaggtggg ggctctcttc cacccgggca
     1321 tgggctaccc tttgagcacg gcaaagaggg tgttttctcc ccgttgaatc aggaccaggt
     1381 gccggtcggg ggacttgcct ccgttcagtc gttccttcac gtcctcgggc gaggaaaccg
     1441 ggtggcgatc cacctcgagg aggatatccc ggggtcgaat ggatgcctcc tccgccgggc
     1501 ttcccggttt cacctgcacc accagcaccc cccggtcgcc gacgattccg tgcctttggg
     1561 ccaggtcggg ggtgagttcc cggagcatga gtccccattt gccttgggcc ggttcctcgg
     1621 cgtcggtttc ccgggcggtc tgggagggag acccggcgat tttggcctcg agggtcaatt
     1681 ccttcccgtt ccgaagaacc ccgacggtcg ccttttcccc cacgggagtg gccgcgacca
     1741 tggccgggag ctgcctgctg tccttgaccg gtttaccgtc gtactcgagg atgacgtctc
     1801 cccgtttgat cccggccttg tccgccgggc ttccccgggt gacgtctccc accagggccc
     1861 ccttaggatc gggcagattc aacgccgccg ccaggtcttc cgtgacgggt tggatggtca
     1921 cccccaagtg cccccgggtg gcctccccgt ggctgatgag ctggggaacg agggtttttg
     1981 ccatattgac ggggatggca aacccgatac cctgcccgtc ggcgatgatg gccgtgttga
     2041 ttccaaccag ctctccttgg aggttaaaaa gaggcccccc cgagttgccc gggttgatgg
     2101 acgcgtccgt ctggatgaag tcgtcgtagg ggcccgcccc gatgactcgt cccttggcgc
     2161 tcacgatccc agaggtgacc gtccggccca gcccgaaggg atttcccacg gccaggaccc
     2221 actctccgac cttgagggcg tccgaatcgc ccatgaccgc cgaaggaaag ggttctttgc
     2281 cttcaatctt cagcacggcg agatccgtct tggggtctcg gcccaccacc cgggccttgt
     2341 attctttctg atccgagagg gttacggtca cttcccgggc tttctccacc acgtggttgt
     2401 tggtgaggat gtacccgtcg gaccggatca ggacccccga gcccgcgccc tcgacccgcc
     2461 ggggtccggg gggacgaggc ccgcccccgg aaaatggacc gaagaaatca tcaaaagggc
     2521 tcccctgaaa cggcattcca tgaaaaccgg ccctttccac ccgggtgatc ttgatgttga
     2581 ccaccgaagg ggccagcttc tccgccagtt ccgtgaagga gggtggggtg gaaggcgaag
     2641 aagaccgcga ctccgtcccc ggggaggttg ccgccgttgc ttggccgggc tgcggagcga
     2701 ggcttcccgc aaccaaggcc gggagcagca agccgacgac caagagtttt gaggggtacg
     2761 agggtacggg tggggacgat ggttcctttc cacgttgcat ccaagagctc catcggctcc
     2821 cggttcctgg attccggttc attgtgccac ctccatggtt gatcttttct tcgtgtggtg
     2881 ctccgtcccg ggaagctctc ctcaccggga cgaaattccg actcccgatg ctctttgcct
     2941 gaaaacataa gggaggaaaa ttaaggggag attaacgccg gattagaatt ttctcatgga
     3001 gggacgagag gggagccgtc ggcggtcgat ggtcgcccgc ttcccattcg tctttcaaac
     3061 cgggaggaaa accttgaagg tgcttccgcg gccggggtga ccgaggactt ccaccttgcc
     3121 gccgtgggcc tgggcaatgg actgaacgat gctcagcccc aatcccaccc ccttttcccc
     3181 caggttgcgc gcctgggtgg tccggtagaa aggctggaag atcttttccc gttcccgggg
     3241 atcgatgccg attcccgtgt ccgaaatctc cgtcaccgcc cagttgccgt tgcgggcaag
     3301 ggacagggta acggttcccc cggcgggggt gtacttgagg ccgttttcca ggaggttggc
     3361 aaaaagctgt tgcagccggg cggcatcccc ggggatcgtc agcggctcga tgaacccgga
     3421 aaccagctcg acggatcggc catgggccag aacacccagg cgctgcaact ccccctcgag
     3481 gagtcgggcc aggtccaccg gccgggtgtc catgcgcagg accccggcct gggctcggga
     3541 aacagtgagg agtccgtcca ccaggcgcgc gatgcgatcg atctcctcga gggcgctctc
     3601 gagggttgcc acgtattctt ccgccgatcg gggcgctctc aaggccacct cgacttctcc
     3661 cttgagtatg gtgaggggcg tctggagctc gtgggaggcg tcggcgctga attggcgaat
     3721 ctgtcggaac gaagcgtcga gccgcttgag catgtcgttc agggtggcgg ccagccggtc
     3781 gagctcgtct cccgtccccg tttgctccag ttgctcggtg aggtgttcgg cggaaatgtg
     3841 acgggccgcc tcggtcatcc ggtccacggg gctgagggcc cggcgggcca gcagccggcc
     3901 gcccccggcg gccagcagca gccccaaagg caggagcccc gccatgatca gcatgaaacg
     3961 gaaccgggtt tcccggagac cctggaggga agttcccacc tggatgagcc cggccacgcg
     4021 gcccccttcc gtgcggggca gggtgagaag gcggatggga ccgctttcca ggccttcgat
     4081 ggtctcgtag gtcgggagac ccatggaggc gttttccagg gcccgggagc tgaggggcag
     4141 acggggacgg cgggcggggg gctctctcgg gtcccttcga cccgagggat ccaggaactg
     4201 aagatacgga tcccacggag aaaagccgaa aaagcggcga aagacctcgt ccacttccga
     4261 gggaaccagc ccggcggctc cgccccggga tttctccgtc agggcctggg ccaccccgtt
     4321 cagggaggcg tcgatctgcc gggacaggct gtaagacagg agcccatagg cggccgcgcc
     4381 caggacgaga agggcggccg tgaaggtcag agcgtaccag aaagtcaggc gggctcgaat
     4441 ggaacgaacg ggcatccgtc actccggctt cagcatgtac ccgacgcccc gcacggtatg
     4501 gagcagcttg ggctctcccg gcaggtcgat cttcttccga aggtagttca cgtagacatc
     4561 gatgacgttg gtggtggtgt cgaaatcata gtcccaaaca tgttcggcga tcatggtccg
     4621 ggtgaggacc cggccggaat tgcgcatgaa ataatcgagc agggcgaact ccttaggggt
     4681 gaggtcgatc ttgcgagggc ctcggtggac ggtccgtgtg gcgggattca gggtcaggtt
     4741 ccccgccagg aggaccggac aacgcgcctc ggttccccgc cgcaggatcg cgcggacccg
     4801 agccaccagt tcctggaagg caaagggctt ggtcaggtaa tcgtcggccc cggaatccag
     4861 gcccaggacc ttgtcttcaa tggcggctcg aacggtgaga agcagcacgg ggatccggat
     4921 ctttcgcctg cgcagttccc ggagaacctc cagaccgtcc attttcggga gttgaatgtc
     4981 caggacgatg aggtcgtgga ttccgtcccc agccatttcc aggccggttt gcccgtcgtg
     5041 ggccacgtcc acggcgtacc cttcctcggt cagtcctttt tggatgaagc tcgcgacctt
     5101 cttttcatcc tccaccacca ggagtcgcat ggaacctcct caaggcatcc ccgaacccgt
     5161 catgttgccg gacgaagctc ctttcggagc aagacttcat caaacccttt tcgaaccaga
     5221 ttggaaagaa ccccgatttc cgtaaatccc gtgcgcaggt agaaatcccg ggccttttgg
     5281 ttgaaagagg aaacggtgac ccacaggttc cggctggtcg gccttgcacg atcctcgatc
     5341 catcggacga cctcccttcc ccacccctgc ccctgccggt ccgggaacac ggcgaggatt
     5401 tcgatatagg gccccaggag ccagggatgc cgaacgcaga ggaccccgca cacccgttcc
     5461 tcgtggaaca tcccgtagcg aaacaggcag gaatcctccc gcagaagata ttcgagcagc
     5521 cattccggac ggtaccccag ggtcttccag gggtccattt caaccagggt gcggcttgcc
     5581 tcgaaggctt cccgggcatt caggcgggat agggcgcagg tgggcagaac ggcggaagac
     5641 gaagggaacg gagggcccgg gcggctcacg gaacctcccc tgccgttggt gagaatgccc
     5701 ccgcgcccgg cggcgtggaa cgattcccgt gggggtccag ggcttccttc agcgtcacaa
     5761 accggtagcc tcgagaccgg agtccgtcca ggatttgggg aagagcccgg ccggtggcat
     5821 atccccgacc gttgatgtga aagatgagga tggacccgtt ggtcgaccgg gataggaccc
     5881 ttcgcgtcag cctttccggg gtgagatgaa tgtcgggatc gccgctggca aaagtccagt
     5941 gcacgacccg gtaccccagg tcctcgaccg ttcggagggt cttggcgtcg tagtttcccg
     6001 cgggaaaccg gaagaattga ggctttttcc ccgtgatttc ctcgatgagc tgcccggcaa
     6061 ccaggacttc ctcccgaacc cggccgggtg ccagccgttc catgtggtga tggtggctga
     6121 gggaatggtt ttccacctcg acgaaatccc gggcggcgat ccggcgcagt tcctcccggt
     6181 tccttcgggc aaacttgccg ctcacgaaga gtgtgaacgg gatttgctcc gagaggaggg
     6241 tatccagaac ggcggcgtcc aggtaatggg gctgtcccat ttcacaggcg tcaaaggtca
     6301 gggcgattcg aggttcccgg gtgggaagcc gggtgatgac ctccccggcg gctggaatgg
     6361 gaaaaaccag gagaaaaacc aagaccttca ggatgtggga tctcgaggag gcagaattca
     6421 ttggaccctt cccgtccccc ggatccacgg tctttcggct tcccaaaacc ttccttccgt
     6481 caatccgaat ctcctgcgga ctgctttcac gtccatgatg gtttcgaaaa aagtcccgaa
     6541 gagtgcgttt cttcgaccct acccgattga attcattagg ttccgttccg agaagaacga
     6601 cttcaaacgg gatcatcatc catcgtttgc ccctcgtcgc catctcccaa gccggcacta
     6661 tatgcgaatc agccgggaca ggcaattttt tccgcccggg gtatccgcgc cggggagggt
     6721 ctggatccga gacgggtccc tttgcccgtt gcctctgctt gccccagaag cattcttgac
     6781 gcccatgcac ttgtgtgcta gggaacacct atgttccgtc ctcattcttt actagaagcc
     6841 aaaaggtgcg agggtttctc ccgcagcctt gtaccgcacg aaaaaggttc ccggccgacg
     6901 gaaggggtag ggtgacctcg tggggaaaat ggagtcggac ctcccgatgc tccgggcccg
     6961 gctcgtggcc ggtttgccgc aataggcttg tgaacacccg agtctccgga ggggcttttg
     7021 ggaatgactg ctttcctgac cagaggatct ttgcatctcg agagaattgc gggaatcctg
     7081 ctgcttgccg tctttgcggt gagctgcgcc aaagtcgggc cggactttgt tcggcccggc
     7141 gcccaggttt ccgatcaatg gatcgagtcc aacgacgagc gggtaagaac cgagatcgag
     7201 gatcaccggc agtggtggac ctctttccag gacccggtcc tggaccgtct catcgagatc
     7261 gcttaccggg aaaacctcac cctgcgcacg gcgggcgttc gggttttgca ggcccgagcc
     7321 gagctgggga tcgtggtggg ccggctctac ccccagtctc agagcctgtc gggttcctgg
     7381 gatcggagcc ggtcgagcga gcggtcggcg ggggcgcccc aggaccccca ccagttgacg
     7441 agttccgcct atttcacgac ctctcaggcc caggtggggg gcgcggctgc ctgggaactg
     7501 gatttctggg gaaaattccg ccgggccgtc gaatccgccg atgcaagcct gctggcggcg
     7561 gtggcggact atgacaacgc gctggtaacc ctcacggccg aggtggccac ctcctacatt
     7621 cagctccgaa cctacgagga gcgtctaaag atcgctcacg agaacgcggt catccaacgg
     7681 gagggcctgc ggctggcgga agcgaagttc cggggaggcg ccaccactca aagggacgtg
     7741 gaacaggcga agaccctcct ggccggcacg gaggcaacca tcccggccct ccagatcctg
     7801 gtcgggcagg ccgagcactc cctgagcctt ctgctgggca aggtccaggg tcaactcgac
     7861 gagatcctgg gcgaatcccg ggggattcct tcacctccac tcgaagtggc cgtgggcatc
     7921 cccgcggagc ttctgcggcg aagaccggac attcgcagcg ccgaactcca ggcggcggcc
     7981 caatgcgccc agatcggagt ggccaaggcg gacctttatc cccacttttc gctccagggg
     8041 agtttcgggt ttctgtccac ggacgtggga cgattcaagc tcgaggacat gttcctgtgg
     8101 aaaagccgga cggggtctct gggtccgact ttccaatggg acgtgctcaa ttacgggaga
     8161 ctcaccaaca acgttcgagt ccaggacgcc ttgtttcagc aggcgatcct gacctatcag
     8221 caggcggtcc tccgggccca gaaggaagtg gaggacgctc tcatcggatt tctccgggca
     8281 caggaacagg cgaagctgct caaggaaagc accgaggccg ccacccggtc gttgaagctt
     8341 gccatggccc agtaccggga aggaatgacg gatttcacca ccgtcctcac ggcccagcag
     8401 gcactcctcg agcagcagaa caactgggtg tccaccctgg gagacattgg gctgaacctg
     8461 gtgtcgctgt accgggccct gggaggcggt tggcagttga gggaaggtca ggatttcgtg
     8521 ccggctccga ccaaggcggc catgtccgaa cggaccgact gggggaacct gctcgatccc
     8581 aaagcccttc cttcttccac ccaggatcga aaggaacccg ccatacggcg gcccgactgg
     8641 taaacagaac aaagaggggg tccaggagca tgacgggaag gagaccggaa tccattccac
     8701 gggggaaaat gatcgcaatc tggggccttg ctgcggcgat cctgggtctt tctttggggt
     8761 gcggggaaaa gcagactttc gtgcctccac cgccccccaa ggtgacggtg agacagccga
     8821 tcaggcaggc ggtcaccatt ttccgggact tcaccggaaa tacccaggcc atcaacacgg
     8881 tgcaactgcg ggcccgggtg gaaggctatc tcgagaaggt tttgtttcag gacggagcca
     8941 aggtcaagca gggacagctt ctgttccaga tccaggccga cacgtacgaa gccaagctga
     9001 agcaggcgga agccgaggtg ttgtcccaga aggccagcct cgaacatgcc gagcgagaat
     9061 atgcccggtt ctcccaactc ttcaaagaaa acgcggcagc ggccaccgac gtcagcaagt
     9121 ggctcttcga gcgggattcc gcgagggcca agctcctgtc cgcccaggcc cgaatggaac
     9181 tggccaagct ggatctgagc tacaccaagg tgacggcccc ctttgacggg cgcatcgacc
     9241 gacggctcaa ggacccgggc aacctggtgg gctcggggga aaagaccgtt ttggccgaaa
     9301 tcaaccagat tcatcccatc tacgtctact tcaccatcag cgagcgggac ctcctggaat
     9361 ttctcgagat ccgccgggcc aaatcccagc aaaagcaaaa accggactgg agcctgtaca
     9421 tgggtctggc caatcaacaa gggtttcccc atattggaga gctggacttt gggtccatca
     9481 ccctggatgc caagacgggt accctgcttt tgcgcggggt ctttcccaat cccgaagggg
     9541 agatctgggc gggaatgtac gcgcgaatcc gagctcccgt ggccacggaa ccggattccc
     9601 tgcaggtgcc cgaaaccgcc gtggcttccg accagcaggg ccgatacgtg ctggtggtca
     9661 acgacaaaaa cgtcgtcgag cgccgggagg tccaggtcgg tcccacatgg aatcaaatga
     9721 tggtcgttaa aaagggtctg acgggaaacg agtgggtcgt tgtgaacggg cttcttcggg
     9781 ccatccccgg tcatgaggtc actccctcca gggaggaagg ccctgcaaaa acgtcgagcc
     9841 cgtcggaatc ccaaggctca taacccgttc ccgtccggaa atccagcctt tcccctccgt
     9901 cccgggggag gtcctcccgt cgcccccagg cggaggcggg accggggtcc ggacgacggc
     9961 caaccaggag gaaccggttc cgtgatctcc gatttcttca tcgaacgccc gattttcgcc
    10021 aatgtcatcg ccatcgtgac catcatcctg ggtggagttt gcctcttcac tctcccggtg
    10081 gcccagtacc cggacattgt cccgcccacc atccaggtat ccacgagcta tcccggggcc
    10141 agtgccaacg tggtggccca gaccgtcggg gtccccatcg aacaagccgt caacggggtg
    10201 gaacggtcca tctacatgtc ttccaccagt gccagcgacg gctcgtacac cctcaccatc
    10261 tccttttccg tgggaacgga tctcaacacg gccctcaccc tcgtccagaa ctacgtccag
    10321 ggggccctgg cccagcttcc cacctcgacc cagtcccagg gcatcaacgt caagaaggtc
    10381 agcaccaaca tcctttgcgg ggtcagtctc tattccgagg acgaccgcta cgacgagact
    10441 tttctcagca actacgccat catcaatctc gaaaaccccc tggcgcgcct gcccggggtc
    10501 ggccaagccc gggttaccgg cgcgggcaag tacagcatga gaatctggct ggatcccacc
    10561 aagctccagt actacaacct gacggtgctg gacgttcaga acgccatcag ccaacagaac
    10621 gtccaggtca tcgccggaaa gctgggcgcc cctcccgttc cggacgaaca ggccttccag
    10681 tacaccatca acgccctggg acggctctcg gaagtcgagg aattcgaaaa catcatcgtg
    10741 aaaaccatcc ggggggaaga aacggcccag atcgtgcggg tccgggacct ggcccgggtc
    10801 gagctgagcc agcagatcta tgccaatttc gcggaggtga ccgggcacaa ggccgggaac
    10861 atcttcatct atgctcttcc cgacgccaac gccatcaagg tttccgaagc cgtcaaagaa
    10921 gccatggggg aaatgagcaa ggagtttccc gaagggctca agtaccaaat cctgtttgat
    10981 accaccgact tcgtcaagga agccatcagc aaggtatatg agaccctcat cgaggccggc
    11041 atcctggtgc tcatcgtgat catggtgttc ctgcagaact tccgggccat gctggttccc
    11101 gccaccacgg ttcccgtgac catcatcggc gccttcgccg ccatggccat gctcgggttc
    11161 accatcaacc tcatgaccct ctttgccttg attttggcca tcggcatcgt tgtggacgac
    11221 gccatcgtca ttgtggaaaa cgccgcccac tacgtggaga aggggattcc ccccaaacag
    11281 gcggcttcca aggccatgaa ggaactgacc ggtcccgtca tcgggatcac cctggtgctg
    11341 acggccgtct ttttgcccgc ggcctttctg ccgggaatca cgggacagct gttccgacag
    11401 tttgccctgg tcattgccgc cacggccgtc atcagcgccg tcaacgccct gacgttgaaa
    11461 ccggcccagt gcgctttgta ccttcgcaaa cagaccaagg aactcaatgt cttttaccga
    11521 ggctttaacc gggtctacag cgtcatcgag gggggctaca cggccgtcgt ccgttggatg
    11581 gtccatcgat atcaactgat ggtcgtggtc ttcttcgtcg tcatcggcat tgccggctgg
    11641 atgttcgccc agcaacccac gggcttcctt cccaccgaag accaggggta cggcatggtg
    11701 ttcgccaaac tgcccgacgg agcatcccag ccccgggtcc gggaggtctc gaaaaaggtg
    11761 gacggcatct tgaagaagac tccggggatc aaaggctggg ttaccatcgg tggatattcc
    11821 gtcctcgacg gggccaacgc tcccaacgta ttcaccaagt tcctcattta cgacgactgg
    11881 ggcaaacgcg ggacggaact cagccaggaa aagattgtgg ccaacctgcg acgctcccta
    11941 tattcggtgg aagaagcctt cctaggggtc ctcatccctc cccccattag cggtttgggg
    12001 cagtcggggg gattccagat gatggtggaa gaccggggat ccttgggact cgaagagctg
    12061 gaaaaggtgg tcatggagct cctgagagcg gccaatgcgc agcccggcct tcgaaacgtc
    12121 gccaccacct tcacggccag caccccccag cttttcctgg acatcgatcg aaccaaagcg
    12181 gaatccctga acgtgcccct caacgacgtc ttcagcaccc tccaggccta cctgggctcc
    12241 gcctacgtca acctcttcaa caagtacaac caggtctttc aggtctacat ccaggccgac
    12301 gctccctacc ggctcaagac cgaagacatc atgggcctgt atgtgcgtaa ccaggacggc
    12361 aacatggtgc ccctgggcac cttgatggac gcacgcatga tcgaaagctc cgagatcgtg
    12421 aacatgtacc agctctaccc cgcggccgcc gtattcggag gcgcggcccc ggggttcagc
    12481 tcgggacagg ccctggatct catggagcag gtggccagga acacgcttcc ccggggagtg
    12541 aacttcgagt ggacggccac ggccttccag gaaaagcagg tgggaagtat ggcctatttc
    12601 atctacgccc tctccctgac cctggtcttc ctcgtcctgg ccgcccagta tgaaagctgg
    12661 accgacccgg ccgccgtaat cctggtggtt cccatggccc tggtgggcgt ccttctcgct
    12721 ctcattcttc gaggcttcga caacaacctg tacacccagg tgggcctggt gctgatgatc
    12781 gccctggcaa gcaagaacgc catcctggtg gtggagtacg cccgggagct tcgggccgaa
    12841 gggatgcccc tcatggacgc cgccgtcgag gccactcgac gcaggttccg ccctatcgtc
    12901 atgacttcct ttgccttcat cctgggggtc gtgcccctct tgggggcccg gggagcaggg
    12961 gccgccagcc agcaggccat cggcaccgtg gtgttcggcg gaatgctttc ttccaccatc
    13021 ctcgccatcc ccttcattcc ggtcttctac gtgttgatgc agaagctgag cggacgtttc
    13081 gggaaaagga aggccgagga gcataccgag gccgtcgagc ccgcgccgga agcatcctga
    13141 gcagggcccc gcaggaccgg gtcttcccgt ccgcgccgga atcggccgtc cgttgcggcg
    13201 gaagccggaa tcccgggcaa gtgcccggat tgcgagtctt gaaaaccatg gaaatgcgtt
    13261 tatgaacgaa aaactcaggc agttctatga agattccaag gcgttgatca cctaccttct
    13321 cgaggaacac gtcttcgaca agatggccga ctgcgggtgc ggcggggtgg atgcctaccg
    13381 gtccgaaagg ttcgacgagc tcattcggaa ggccgcaaaa tccctcggag agttcgaacg
    13441 ggagatgagt ccccccggcc cctgaatccc cccttccttc tgctttgagc cggatggaac
    13501 ggacaatccc ttctcccggt ttgccgggac catgccggaa ttgtgtgacg gcgatcttct
    13561 ccgactgcag tatctgaaca acctctcctt ggatcccgaa aaggtgatca ttgccatgga
    13621 tttcggcagg gagttgttcg actactcgct ggggtgcatg ggcgcccccc gatcccctcg
    13681 aggcaccgga cggcgcgaat tccgatccgt taaggcgatt cgccctcctc ccggcgcgag
    13741 gcggaggccg gatgggaggg gttaagcccc gaggaggatg aaaagcccac ttacggacac
    13801 gttctagtcg ttgtccttga tggtgaccgt ggcctgggtg ggggttccca aatcgtaggc
    13861 aggcttgtcc accagggtca ccttgaccgt ttcattcact tccttcttgg tatcgttctt
    13921 gggtttcacc ttgaccaggg ccgtcttgac tcccgcggcc atggtgacct tgccgggaag
    13981 agccgtgtag tcgactccgg caaccgcgct ccccgcaact ttgtagtaga tggtcaccga
    14041 ggtggcggcc aggtcccggg cgaacttgaa ggtcccggtg gtggccccgg cctcggtggc
    14101 tacattgtcc ggagcggtga cgctgacctt gggaagcttg gaatagatct gggagaggtt
    14161 cgaggtggtg ttcgccttga ggcccgtgat ggtgaaagaa tcgggaacgg tgctcatggt
    14221 gcgcaggccc tgggtgggct gcagggcggc ggccaggttt gccgtccagt tgccggaggc
    14281 gtcggccgtg acgaactgca ggggagtgag gcattccttg acggaatcca ggtcgtccag
    14341 gaaaagctcg atgatgcatt ccgcacacac gctcccggcc ccgctggtgc ctttcaccgt
    14401 cgtgccgtcg atcttggtga cggcggcggg tttgaaaccc ttgagggcgg cagggaccat
    14461 gggaccgtag gcgatcgcgt cttcggcaaa gccgttgtcc ccctgtgccc ccggccactg
    14521 ggtttttctc ttgatgacgt tggaccggag ggcgttggat tccacggaag agttcatcaa
    14581 gacggcacta agcccggttt ccacaaaggt gttgttcaaa acctgcagct cgtcggggcc
    14641 ggaccccagg tccagtcccc ggccgcagac gcccaccgcc ttcccggccg aatccaggcc
    14701 gacgacgttg ttctggatga ggtgattcgt gccgggatag accttgatgg cgggcccttg
    14761 agtggaagtc tcactgagat ccaggaaaag cccggcaaag acattgccgt ccccggcgtt
    14821 gggccctccc acctggttgt tgctcccggc aacgctgatg ccgctgcccc ccacccagcc
    14881 cccgctgctg caaggatgct ttgtgaagcc ggacggcagg ggaatggtgc cgttggcccg
    14941 ggtgcccacc caattgccgg taaactgatt gtggctgccg ttgatgctgc aggcaacgcc
    15001 ccagaacccg gcaaacatgt tgcggctgat cacgttggaa tcacaaccgg aactcaccag
    15061 cacggcgctg ccgccctcgt aggtctcttc atccccggcg ctgagaagag ttccgtcgtc
    15121 cgaaagaccg aaccaattgt cggacacggt gttgttgtgg ccggcaacgg agatgtgagc
    15181 ggtcttgaag ttctgcatgg ccagaccgat cacggcattg gcgtcgtagc gcatgacgaa
    15241 tccgtagttc ttggccttct ttccatcgag gatgatcttg ggagcgccgc tgcgcccgcc
    15301 cggctgggtg gaaccgtcca cggtgacctt cccgttgagg tcccggaggg ggttcagtgt
    15361 ggtcccggtc agttcgatct tccacgcctg gaggaggtcg ttgtacccgg aatccgtggt
    15421 gggaatgtcg aagttgatga gtacgggccg gtcgccgtcg cccagggaat acgcctggtt
    15481 gacggcccga cgcagggtgc agggagtggc gttctggcac gtcttgctgt tgccgtcgtt
    15541 gtagttgtct tcggtgctct ccacggtgat gacggtcacc ggatccgcgc caaaggccac
    15601 ggaaacgggc aggccgaaca gcagggaaac caggcacgcg gcaaggatcc atcgaaacaa
    15661 gggagggttc atgatctcgc tccttttgtc gaaaactgcg gaagaggtcc acgacaaaac
    15721 tcatgacgca acggaaaacc ggaaatctca ccggcactgg aagcctcgga tccgaggagc
    15781 ggaagacatg agaatacggc atgtgcaagg tagactcagc cctttcccgt ggcagacgct
    15841 cggcccgcgg ctcctccccc gttcgcccgg gagagtccgg cggcagatcc ggcccctcga
    15901 cctgcggccg agtgttccca tgccccagaa actttcgaag gagggattgc ctaccctatg
    15961 ggcggactat acacttgccc ggatccccat ggcaacggaa aaacccgccc ttcgacgaat
    16021 tgatccgatt ctgtcttgat cttgtggtgt tatcaggtat ttcttgcttt tggactcata
    16081 gtcggtatac tacgggcggt cagtgatccc accccgccgc ttgacctcca cgagagccaa
    16141 gaactgcgaa tggtgccgga cacgccggga accagcgtgc agggagtacc atgagtaccc
    16201 gaaattgaga ccacataacg caaaatggga gccttccgtt gaaacttccg tccctaacaa
    16261 tcggtgagct agtcacaaag atccccatcg tccagggtgg aatgggcgtg ggaatttccc
    16321 tctcgggcct gtccgccgcc gtggccaacg agggtgggat cggggtgatc gcaaccgcta
    16381 tgatcgggtt gaacgaatcc gacctttact ccaactacct cgaagccaat atccgggcac
    16441 ttcgcaagga aatccgaaag gcacgggaaa agaccaaggg gatcctcggt gtgaacatca
    16501 tggtggcgtt gaccaatttc gccgacatgg tcaggacttc catcgaggag gggatcgacc
    16561 tcatcttttc gggtgccgga ttgcccatgg acctgccgaa atatctgaag gaaaagtcta
    16621 aaaccaggct ggtgcccatc gtttcctccg ctaaggccgc tcagatcctc tgcaagaggt
    16681 ggttctcgag attcaagcgg ctccccgacg ctttcgtggt ggagggtccc atggcgggag
    16741 gacacctggg cttccgcgcc gaacagctgg atcatcccaa ctatgccctc gaaaagctca
    16801 ttcccgaggt cgtgcaggcg gtcgagcctt tcgagaagga acaccggaca aagattccca
    16861 tcattgccgc gggaggaatt tataccgggg aggacatcct caagtttctg gagttgggtg
    16921 cgtccggggt ccagatgggg acccgtttcg tggccactca cgaatgcgat gccgacattc
    16981 ggttcaagga agcctacgtg aaagccgggg tgaaagacct ggtgatcatc aagagccccg
    17041 tcgggcttcc cggccgggcc attcgcaacg agttcgtgga agaaatggaa cggggcgaga
    17101 aaaagccctt caagtgtccc taccactgca tcaccacctg ccgatgcaag gacagtccgt
    17161 actgcatcgc cctggccctg gccaatgcca agaaaggaaa cctggccaac ggattcgctt
    17221 ttgccggggg caacgcctac cgggtggacc gcatcatgtc cgtgaaagag ctcatttctt
    17281 ccctgatcga ggaatacgac caggcttcct gccccctttc atcccaaagg tcgtccggtt
    17341 ccatggcctg agggcgattc acgctgtcct ggagtcgtca tggccccggg ggacctcacg
    17401 gggaggggca aatccggctt ccgcgccgct ttgctctcct tctgtcgatt cctccgggac
    17461 cggccggact ccccgccaat ccacccattc ggcatttgca gttcccctcg agggaggata
    17521 taagggaagc gttctcgatc ttcccccatt ccggaccggg aagttttggc tcggcccgaa
    17581 tccacaccga agcatcaccc gcacaggagg cggcccatgt ccacggaacc ttcgtccaac
    17641 gatatctccc ctgcaaacaa gtggcgcttc tcccgggttg ggggcttcga ccaggtttcc
    17701 ctgcaaagcg ggaacgacct tctcaacctg gatcagctgg acccaaaact gtgggccgcg
    17761 ttgagctgtc ccactcacgg tctggagatc gatgcccgga ccctggagct catcgactac
    17821 gacggggacg gcaggattcg ggtcccggaa ctgctcgagg cggtcaagtg ggctacctcg
    17881 ttgttgaaga accccgaaga cctcctcaag ggggaagact cccttccctt gtcctccctg
    17941 gacgacagca ctcccgcggg caaagaactc ctctcctccg cccggcagat cctcaccaac
    18001 ctggggaagc ccgagagcac gaccatctcc ctcgaagaca cgggggatac ggtcaaaatc
    18061 ttcgccaaga cccgcttcaa cggggacggc atcatcaccc gggattcatc ggaggaagag
    18121 gccgtaaagg ccgtcattgg aaacgtcctc gagtgcatcg agcccgaaac ggaccgaagc
    18181 ggttccccgg gtgtttcccg ggaaaaggtg gacctcttct ttgccgaaac ccaaaagttt
    18241 gccgactggt ggaagaaagg agaggaagat ccccacagcg acgccctccg gcttttctac
    18301 tccgacggcg gggcggtcgc cgcctttcaa gccgttcggg ccaagatcga tgattttttt
    18361 ctccgctgcc gcctggcaca attcgatcct tcggccgccg aggccctcaa tccttcggtg
    18421 gaagattacc ggctgctcac ctcgaaagaa ctctcccgat cgtccgaatc cctgggatcg
    18481 ctcccattgg ctcacatccg aaccggggcc tctctgcccc tgacggaagg actgaacccc
    18541 gcatggacgg cccggatcga atccttccgt tcccgggtgg cggcgcccct tttgggagaa
    18601 aagacctcgc tcaccgatac cgactgggag gtcctgtcgg aaaaattccg ggcctatgag
    18661 tcctgggtcg ggagcgagga aggaaccacc gtcgagaaat tggggatcga ccggatccgg
    18721 gagatcctct cgggaggcta ccgggaagcc atcctttccc tcatcgagca ggacgaggcc
    18781 ctcgagcccg aagccaatgc catccagtcc gtggacaaac tccttcgcta ttaccgggac
    18841 cttttccgcc ttctcaacaa cttcgtctgc tttcacgact tttatactcc gggtcggaaa
    18901 gcgatttttc aggcggggtc cctttatatg gacggacgga gctgcgactt gtgcgtgcgg
    18961 gtcaccgacg tcgccaagca cagcacgctt gccaccctca gccacaccta tctcgcctat
    19021 tgcgaatgca ctcggaacgg agaaaagatg cagatcgccg cggccctcac ccaaggggac
    19081 ggagaaaacc tccggccggg cagaaacggc gttttcttcg atcgacaggg acgggactgg
    19141 gacgccacca ttgtgaagat cgtcgagcat cccatcagca tccagcaggc attctggagc
    19201 ccctaccggc gcttggcccg aatggttgcc gagcaggcgg aaaagttcgc ttcggctcgg
    19261 gaaaaacaag tccaggcttc cgccgccggg gcgatcgagg cgggcaagaa gcccgaagcc
    19321 cccaagcccg gtgcggaaca gcccttcgac gtggccaagt ttgcgggcat ttttgccgcc
    19381 atcggtcttg ccgtcggggc catcggaacg gccatcgcct cggtcatcac cagctttctg
    19441 gggctcaagt ggtggcagat gcccctggcc gtggtgggaa tcatcctgat gatctccggc
    19501 ccctcgatga tcctggccta cctgaatctt cgcaagcgaa acctggcccc cattctggat
    19561 gccaacggat gggccgtcaa caccaaggca atgatcaaca ttccgttcgg ttcgaccctc
    19621 accaatctgg ccaagcttcc cgaaggggcc cagcgcactc tcctggatcc ttttgcggaa
    19681 aaagaacgac cctggaagct ctatctttgg atcgccgccc tggtcgcggg gatcctggtg
    19741 cttctccagt cgggctacct gggccaatgg ctgagacttt tgttctaagc accaacccac
    19801 acaacaagcg gtcggtgggc ctccatgacg acagctccga ccctgcccga cgtctccctt
    19861 gcccgccggg agctccggga gctcctgcgg gggtcctgca tcctgagacc cggaccgggg
    19921 caacccttgc ggactcccga cggagaaccc gccctgtggc tgttctacag ctgggatgcc
    19981 accctcaccc ggcgggggat gaccctcgcc gccgcgtgcc tcctggaagc cctctccagt
    20041 tttgaagcct ctcagctggc caccgtaggc tacacgggac tccccctcct cctggggtgc
    20101 atcctttacg gcaaggatcg ctacaccggg ttgtgcgtcc gggatgagcg caaggggcac
    20161 gggtccctgc gccggatcga aggtcccggg gaccgctccc gccccgtggt tctcatcgac
    20221 gattccctgg tttccggatc gtccctgtgc ggcgccgtcg aagccctcga aggggaaggg
    20281 ttccgggtgg aaggagccgt ggccctggtc aatttcccgt ggggaggcgg catcgagcgc
    20341 ctcaacgccc tgggctaccg ggtcgagtgc attttcgatt ccgtccggga cctgcaaatc
    20401 gatgaacccc tgccctccac ccctcacctg gacctactcc ccgactcctg ggatcatccg
    20461 cccatcccgg acgggctgca cccggccacg gcggcccgca tgaccatgga acactacctg
    20521 cgaaccggaa aggtcccccg gcctcccggc cgcatggata gcaactacga cggtcgaggg
    20581 ggcgtgtttg tgagcctgag ggagagggaa accagttttc ggctcgcccg ggacggtttc
    20641 taccatttcg atgcccggga ttccgacccc tgccgggacg tggtgttggc tgccgtcgcc
    20701 acggtccgag gggccccgga actcacccgg gagaccctgc gaaccttgaa gatcgccgtg
    20761 agctttctgg gtccgctccg ggagacgatt ccgggaggcc tggattttcc ccggtacggc
    20821 atcgtggtgc gcagcaaggg aggcgagcgc atcggaggcg ctctgcccaa caccgagtac
    20881 ttcatcgggg agacggaaca gtaccggcat gcccgcagcg tcaacgccca ggtgggggat
    20941 ttcgagcccc acgacctttt ccggcacacg gtgaccaagg ccgtggaacc cggtgcctac
    21001 tggctgccct tcggatcctc cgcgaattcc caggaagatt ggacggaaaa agaagagatc
    21061 ggcaggcggc tcacccttcg agcccgggag atcctggcgg ccctgccaaa cggcccacag
    21121 ccgggagcga accccgtgcc cgacgacctg atcccggctc cccttttctc ggtgggcgtg
    21181 acgctttttc accggggttt gctgggctgt ggactgagct ggggggatac cctggatcga
    21241 tgcctgcagc aggcggtgga gatcgcctac ggggatgagc ggttttccgg aagcgaccgg
    21301 ggattgcagc ccaacaattg ggccgttgcc gtctccatcc ttcacgaccg ggaatggctc
    21361 ggggaggcat ccctcgagga agcctgcacc cggatgcgcc gtggtctcga ttccctctcc
    21421 atcgagtacc ggggaggcgg cgatatgctc cttcccttcg tttgcgtgca cgagaacctg
    21481 tccaaggagc aaatgggcga agcgctcctc gagaaggcag gcaatccagg acctcctcac
    21541 cactgggccg tttatcctac ggccacctgg atggaaaagg acgggaaggt gtcccgggtg
    21601 aggttcgggt ttccggaccg gttgggggat ccttacggac cggatcgatg gagggaggat
    21661 gctgctcttc tggcgtccta cctccttggg cacctgggtt ccgacggcct gcccgagtat
    21721 tcctacgacc ccatcaccgg gcaccgggta aaggaagccg cctccccggt ccgccccctg
    21781 cacgccttgt gggccctggt cgaggcggga cgggtgctgg aatgctccca atggtgccgc
    21841 agggctcgcc ggggcctcga gctctgcctc gaacatgtcc gggaggagaa aggcgggcgt
    21901 tggaccctgg gattggcgga ctacgggaag gacctcctgg ccgacgcggt gctgctcctg
    21961 gccctgaccg ccgccgggga agatcttcgc acggacccca agacggaggc cctggcatcg
    22021 aacctccgct ctcttgtgca gcccgacggc cgggtgacgg aaatgcccga ggccaggggg
    22081 atggattcgg accacgacat tttccccgca gtggtcttgc tggctttggg ccgatacgcg
    22141 gaaaacacgg gaaagccttt cccggcacaa gggatcgagg ccgccctcga ccggtacgcc
    22201 cgacgctttc gcctggttca tccgtgggcc atggtgggct ggcatcccca ggcgtggaac
    22261 gccctgcatg cggtctcggg gaaccccgcc atggcggcct tcgccgggga agtcgccgac
    22321 tgggccctgg catggcagca tgaagagaac ggctcttttt tgtgcgacct cgaacccgaa
    22381 ggctacagct tccacacggc ctttgtcgcc gaaggagtgg cggacgcggg aaggttggcg
    22441 gcccgccggg gggacctttc cggctgcgac cgctacgccc gatcctgttc ccgggcctta
    22501 cgcttcatga accagctcat catccgggaa gaggacacct tttgcatggc gaatcccgcc
    22561 gcgtccctgg gaggggtgcg gggggccgtg tttctctcgg aagtgcgaat cgactacgtg
    22621 agtcataccc tgcttgcttt ggtcaaggca ctggaggcgt ttccggattt cccatgagtt
    22681 gcctggggcc ggtccgtcga atgcctcaga ggagggagcc aaatggtcaa gagtccatgg
    22741 aaggaactgg tggccgctgg agcggtctcc ttgttgggcg tcacggcccc ggcagccgag
    22801 gatgcccctg cccgggccgc cggggcgtcg tcggtcatga cggcccaggc accagcggga
    22861 caggattcga tgacggagcc ccgagggcag gtccaggccc cggagaggcg ggtcgaggca
    22921 cggtccaagc ccccccgata caagccgccg gacaaacggg tggaagcccg ccccaagact
    22981 ccccagtaca agccgcccga tacgcgcgtc gaggccaggc ccaagggtcc cgtccaaccc
    23041 cctcccgggc gtattgtcga agcacggccc acccccccga aagactcgaa gcgggtggag
    23101 atgcgataca agactcccga ttccgggaag aaggagccgc gccaccggag ggaaggtccc
    23161 caatccgacg cctggagaaa gagccccggc cccggaagct cgaaacctca gccgcgggtc
    23221 gagatgagaa agaagacccc cggagacccg ggcaaccgga agatcgtcga gatgagaccc
    23281 aagccgccgc cgacccaggg aaaccagaag cgggtggaga tgagaccccg gccgccgcag
    23341 aatcccgagg agtgaaagcc ttagggcggc ttcttgaaaa tgcgggtccc ttatggggct
    23401 gaaccgtccg ccaattcgtc tttcacccca gggaatcgac tttccggtct tttgcggtga
    23461 gcgggcaggt gcggcatgcc ggaagccgcc gaacgtcgtg ccgaccttct tctggtccgc
    23521 gttcggcgtc accggggttt tctcggcctc aaaggccgtg atacgggttt tccggaaggg
    23581 ccaggctcag gtagcgcccg tccaggaaca ccatgagccc ccggtggagg gattcttcga
    23641 gaaattggac gatccatccg gccggcccgc ggaactgccg ctccacttcc cccagggtgc
    23701 gagagtctcc gcaggattcg tagagctccg cttcccgccc tgccagccga tattccaaga
    23761 cctccccgcc ccgaccgtcc cgtatccgga tggatgaccg ggtcttggcg tacgtcagag
    23821 acggtcgtcg ttccgaagcc caacgttcct gccactcccg gaccgttcga tagatttcgt
    23881 cgaaagcttc gtcgggaagg gcgccttgga tctcgggcgt aaaaaagtag gctactcgat
    23941 cccgggcaaa gcgatccgga ggccacagaa atcggtagac ttcttcggga ttcaaccgcg
    24001 tgatgccaaa agccgcagga tcgacgaaca tgggggaacc gcggtcgggc cgagccttca
    24061 ccatggcgta tgggggctgg aggtgcccga taaggccgaa gagatcacac tgttcccggt
    24121 agtcttccgg ggtctccccg ggaaaaccca ccaggatgtt gtagaggttg tcgattccgt
    24181 ggtaggtggt ccacttgagc agttcgaggt tttcccgggc cgtggttcct ttcctcatga
    24241 gcttcagaac atgggtcgag aagctctcga tccccgactg gacgcagcag aggcctcccg
    24301 cgcgcattcg agcgatctgc ctgcggtcca ggcttgccct gacggcataa acgatcttga
    24361 agtccgcacg agcttccgcc aggtgcccga agaccccttc cacatagtcg ggatcaagga
    24421 cgttgtccac ggcatccagg ttcaggatgt tgtagcggct cgccagctcc tcgaacatct
    24481 cgaggacccg gggcgcgctc ttggatcggt atcgaatgcc cacccggttt tgcccgcaaa
    24541 aggtgcaatg gcttctttcc ccccaccagc atccccggga cgtctcgatg ggaacaaacg
    24601 gctcggcggc cccttcataa tgggcgtacc ctccttcttc cctggcccga aagtactcgt
    24661 cgtagtcggg ggtcggagtc cgatccatgt cctcgaggtt ggaagcgcgg cccccctcga
    24721 aaataccctt cccttcccgc ccgaaaaggc cgggaagtcc ctccatggac tcacccccgt
    24781 agaggcgtcg aaccatttcc ggaagcactt catcggcttc cccgcagtgg acgaaatcca
    24841 cctgggggca gttgcccagg atttcccggg ccaggtcctc ctccatgacc ccgcctccca
    24901 agataacggg aaggtcggga tgccgttctt tgatgcgccg ggccagagcc aggcttgcca
    24961 tttgctgctg gaaaaggacg gtaaacccca ccagcccgaa ccggctccaa tccgtccaat
    25021 ccaggcaccg atccaggaag gtcccggtga gctcccggcg aacgcgcagg aggtcctcga
    25081 gggtgcagcg ggcagaccga cacagcccat cgagctggtc tccataggtc tcgaggtaga
    25141 gatcttcgtc cctgttttcc tcgaaggcgt gagcggccca gatccattcg cccgccaggc
    25201 acgtgggcac ttccgccacg gcttcgtgga gtctccagcc gatcagcgac ccgaagtagg
    25261 cgtagaggga aaaaacctcg acggcaaacc cggctctctc gagggtaggt ttcagaaggc
    25321 ccaactggag gcatggatag cgggccgaag aggtcggcat actgacgagg gcgacggggg
    25381 gaaacgggcg gaccttcacc gtaaggggct cctgggggaa aggcaaatcc ggtgacaagg
    25441 catgggttca ccccccggga aacgggggac ccggaggaat cgggggcggg ttgtccgagc
    25501 gggttgtttg cggcccgaat ccggtatcca tccccgaatg tcggcggtcg actacggttg
    25561 ttcttccgga tttgcgccgg gcaacggcca ggatgcgcgg atggtggttc cctttccctc
    25621 ggccgccgtg atgtcgaagg tacccccgga aagctccgtt cgctccctca tgttcaagag
    25681 tccccacccc cgatccgtgc tgttcgtgga aagggccttt tccaggtcga agcctcggcc
    25741 gttgtcccgg atgagcagat tgattctccc gtccttcttt cgaaaagaca ggcggacctg
    25801 ctccgccccg ctgtgtttga cgatgttgtt catggcctcc tgggtaacgc ggtagaccac
    25861 gatcttcaag gaatccggga tttcgtgctc ggctatatcg aaccgctgct cgatgcgaat
    25921 ccccgagtag atggtttgaa accgcctgca aaaccaggaa aacgtcgcca gcagccccaa
    25981 gtcgtccagg atggtcggcc tgaggtccat ctggatacgt cgacattcct cgatgctttc
    26041 ctggatgacg gggatgacgg ttttcaaggg tcccaccctc ccgtcgcccg atttctccat
    26101 ccgttgcaaa gccccttccg tcacgaactt caccgcgctg aggcaggctc cgatggtgtc
    26161 gtgaatctcg ccggcgactc gctttcgctc ggcctcgtgg gcgaacagga gcctcgaaga
    26221 caggcatcgc agggcttcgt tggcccgctc gagctcgact gtgcgttcct tgacccgctc
    26281 ttcgaggacg tcccgcgatt tccggagctg atcgatgagc tggatccgct ccatggcgat
    26341 ggccacctgg tcggtgacgg tcctcatgag ggccaggtcc tcgtccgaaa accggctccg
    26401 ggtccgggtg ccgaaggaga gggtcccgag gagtcggccc tgggcgatga ggggatggca
    26461 ggcgtaggcc tggattccgt aggactggac cagttccgtt cgaggatcgg gagtgcaggc
    26521 aatatcctcc accacgatgg gtttcagccc ttgggccacg cagccgcaga cggccacccc
    26581 gtaatcgagc cattcgatct tgcgggcttc ttcttcgggg atcccggcac aggcgttgag
    26641 gtggagcctg ccgacctccg ggtccacaag aaaattgaaa aaggcctggc agtccaaatg
    26701 ctccatgact tcgcggcaca actcttggac gatcccctga gggtcgtccg agcccaagag
    26761 ccggttggcg gtatccgaaa gaagccggta ccgggcctca ctcgcccgca gggcttcttc
    26821 cgcccgcttt cggtcggtga tgtcgtagaa caacacggcg aaatgcccgg gttccgtctg
    26881 gaaagcccgc acctcgtaat gccggcccaa ggacccgctg tagtgctcga agtgatcgaa
    26941 ttcccccgtg agagcaacgc ggccgtatct ctcgatccaa aagggttccg tatcgggaag
    27001 aacctcgcga acggtgcggc ccacgaggtc cgaggatcgg agccccgtct gagcttcaaa
    27061 gccgggattg atctccagga accggtaatc gcaaggacgg ccgtcctcgt cgcagacgac
    27121 ctcgtggagg gcaaacccct cgttcatggt ctcgaaaagg tttcggtagc ggtgttcgct
    27181 ttttcgcagg gcttcctcgg cctgcttgag gtcttcgatg tcggtgttgg acccgtacca
    27241 cttgacaatg cgccccttcc ggtcccggac cggcacggcc cgcgtctgga accagcggta
    27301 gaccccgtcc ctccgacgaa tccgaaattg gacgtcaaac gcctgctccg acgcgacggc
    27361 cccgttccag gccgccatga gggagtcgcg gtcctcgggg tgaacccgtt cgagccagtc
    27421 gaaacccagt tgttcggctt cgggaacccc cgtgtattcg acccattgag gactcaggta
    27481 atcacattcc ccgtcgggtg tgcaggtcca gaccaggttg ggcatggatg cggccagcag
    27541 ccgccaccgc gcctcgctct cccgcaactc ctcttccgct tctttgcgcc cggtgatgtc
    27601 cctcaagatc acctggatgg ccgagccttc ccgatcctcg aagggactgg ccgccacctc
    27661 gacgttccgg agggccccgt ccaattggac gatgcgtgcc tcgatgagcg gcacggcctt
    27721 gccctcgcgc aaacgccgaa tgcgctcgtc cagggtgggg tgatattcgg gatggaagag
    27781 ttcgtaaggt gacttgccca ggacctcctc gggatcactg gccccgagga gccgaaccgc
    27841 ctcgggattc acgaagacga tccggttgtc gcggttgacg aagacggcgt ccggggacat
    27901 ttccaccagg ccgcgatacc gttcctcgct tgcccggagc gcgtcctcca tctgtttgtg
    27961 acggaaactt tcaatgatct cccatctgcc cttgcgcttg atgagggcga actggtggtt
    28021 ggcgaccacg tccatgattt ccgaggcacc gcattgcttc aacgagtagg tgcacaacgc
    28081 catcatggga tagcggccga tgacgttgtt gatcgtttcc tcgtaacggg tgaaatcgtc
    28141 ccaatcgtcc gcctcgagcc agaaagtgtt cccggaaagc cttaggccct cgaagccttt
    28201 ttcccgggcg gcctcgagct tttcgaccca gccccggagc acctcctcgg cggaaaactt
    28261 tcccgaccgg atataccatt ggctgtagtc gagaatttca atgcggccct cccggaggca
    28321 gcggtccgca tcgggaagcc cggtcctgat cgcggccttg gcctcctcca catccagggg
    28381 cgccgaggtg atccacatgc agaactcgtt gcccgccagg ccctcccgaa aatagggtac
    28441 gagggtctcg atcaggtcca ggccggtctc atagaattgg cagaaatgag tcccccaggg
    28501 aacatcgccg atgatctcga ttccggagca tctcaaggca ttttcctctt tcgataaatc
    28561 cctttccagg acttcactga ccgtttccct ctcgcgggcg ggggccccaa accggactct
    28621 ttgggatgga ttggacttgc gaaatgagtc tcacatttgg ggatgatact tttccgggcc
    28681 gtgtccgtca atacaagtcg ttttattccc ggccgggtgc gccaaacagg ctccacggcc
    28741 gttctccaat ggagaagttc tcccggggtc ccgtcccggg acctcacatt cccgatgaga
    28801 acgcagaaaa ggagaatccg cctaaggcgg ccctgatcga cgcggccacg ggcagatggt
    28861 ccatgtgact cccgaccccg tggaagacac cgcccgccgg ttcgaaatcg agcaggggga
    28921 aagccgacca ggaccgggtg ggaccccccg ggaagaaagg ctgcgccgcc ctggccaaag
    28981 aaaactcgag atgcttcacc ctttgattgg tccgagggga tcagggaacg agttcgaccc
    29041 tctggatgag gacgggtttg gcaggcacat tccggtgggg acccaccgtg gtggtcggga
    29101 ctttcttgat ccggtccacc acgtccattc cctcgatcac cttcccgaac accgcatagc
    29161 cgaacccttg gggggtcttg tcccgctggt tcagggaatc gttgttcttc acattgatga
    29221 aaaactggca ggtggcgctg tccaccatgc ccgttcgggc catggcgatg gttccccggt
    29281 cgtttttcaa gtccggtgcc gcctcgttct tgatgggttc atgagtcgac ttgggcttca
    29341 tgtccggcgt cattcctccc ccctggatca tgaaatcgtc gatgacccgg tggaagatcg
    29401 tcccgctgta aaagccttcc tcgacgtatc tcaggaaatt tctgaccgtg acgggggctt
    29461 tgtccggcca gagctcgata cgaatcgttc cttccgaggt cttgagagcc accacggggt
    29521 ttttcccctc ggttgcctct ccttccgtcg ccagacaaac gttccccaga aagccgaggg
    29581 cgacgatgag tcccaatacc gttccgattc tgcgattcat ggcaagtccc ttttcaaaaa
    29641 tcggcaaacg aagctgcatg atgggccgga gcccaacgcc gatcgggtcc gttttcctct
    29701 acccccgaac cgggcacaaa cttctccccc gggacccgcc cgagccggag ctcgggctcc
    29761 cggcccccgg gttaaaggtt tccacgcttt tgccgttggg tggtttttcc tgcgccttcc
    29821 ttctcaagat gcccgacaac ctttcttaga accgcttctt accctttcag cagttcccga
    29881 cattcctgct gtccgtgtgt ccgattccat ctcggagctc ggagagacgg cagaggaaga
    29941 ctcgccgccg aaccgcggcc tcttcaggag tcagttccat cgtaatcatc ggctgcctcc
    30001 tttctttggt gcccggcccc tgactgcaac ctcgctgtgg gcgacgggac cgtcggatat
    30061 ggacagggat gttcatcata tcaaacccac ccgggcacgg aaagtgctcg gctatttcca
    30121 gctccattcc attgacttgg aaccttgatc ttcccgtcga ggttcccgtt actttcattt
    30181 ctgcagccga cgaaaggggt ccaccaaccc cctaatcgga tttggagaag tttgaacccc
    30241 atcttcccgg gaatcccccc tgggcggaat acaagaaacg ggaccgggaa attacggggt
    30301 tcaccccatt gagagggcga tcacgggcac ctaccattcc cgggaacggg ttttttgagg
    30361 gtttctcggc gccaaatccc gggaggatcg atgggaaaaa gcaaaagagt tccgaaccac
    30421 ggcagggctt tctccacggc ccgtttttcg ccgggtgctt ccgggaagcc gtaccacggg
    30481 actctgccgg gaggaaagtg ctttcgggag aaggggagga ttcccggaat tgcccgggta
    30541 ctttggctgc tggctttcct tgtcgtcgct cagtgggaaa ttccttcctg ccgtgccgcg
    30601 tcccggaccc cgcagagctc gctctacccc ctgtccaaaa tccctttgac caaaggaaga
    30661 ttggcatccc ttcccgcaag tcggggaccg gtccacatgg gcattggaga ggcggaaatc
    30721 cagcccgtcc ggttgatctt ggattctccc cccgaagagg aagttcaggc gaccctcgag
    30781 cccgcgggtt gggagcacgc cctggaagta agcctgtgga aagccgaatg ggtccccggt
    30841 tccgaaggga accggggcag ggttcccgac atcctggtgc ccgtcgagtt cgcaagccca
    30901 atggttttca gcgaaacgga caatcttctc tttctcaaga tcaagtccct cccgaacagt
    30961 gctggtttct atgagctcat gctcaccctt tcgggagaga ccacgtccct caagtcgaag
    31021 gtgcttgtgc acgtccacaa tgtcccaata tctttgccga atttgctcat tcagggttgt
    31081 ctgaaagagg acaagagctt cgatctcatc gagttcctga gagactacgg gtttaccggg
    31141 atttgcggcg tatccacgga atggcacgtt cgaagacgtc cggaggggtt caagaagctg
    31201 tgcgatcgaa tcctttccct gggatacaag gcgattcgca tccccaacaa gaccatgtac
    31261 ggccacagac gtgccttccc ctacccggac agcgcggacg gatacaaaca atggctcagg
    31321 gatgaagtag attatttgaa caacctcgat gacttgcttc aggataagaa atatcgggat
    31381 gttttctctt tccgctgctg ggatgaaccc caagccgatc aatacaacca agtctacaat
    31441 acctctcgac tcatcaaaga aaaccaaccg aatttgaggc tggaactcac cgaaccgccg
    31501 gactccggcc tatcgcccta tgtggatacg tggagtctca atatccgata cctggaccgg
    31561 ccgacggtac tcgaggcaaa gacgggtgga aagaagtgta ttctgtacgc gaattcactg
    31621 catggagtca accgcaagtc ctacacctat gacatgaggg ccattgggta tttgctctgg
    31681 aaattcaggt tggacggtta ctacttttgg ggcttgaacg attgggaggg ggatgttcaa
    31741 gaaatgtcca cgacgggagc tcccagggaa tacaagagag ggattcttct ctatagactg
    31801 aaggattccg gtaagatcgt cccttctctt cgcctggaac tttttcggga cggactggaa
    31861 gacctgagcc ttctcaagta cattgcgggc aagggggagt tcaagggact gagaaaggaa
    31921 atccaccgga agctcaagac cttccagatg gtcgacaagc aggccgtcgt cccggacatg
    31981 acaaaatacc atcacaggat cttgtcgctc atcccgtgag cttcccgggg cgggatggaa
    32041 agacgggagg ccggaggaat ccgggaaggg ccggggtcac accgggcatc cagcccacag
    32101 gtcccggccc gtcccccttt ccatggaatg cgaccgctct tggggcggat ctagtctttc
    32161 acccgggtgg cacgcactat acggatcgtg ttttccaccg tctccacccc cttgatggac
    32221 cgcacgatgt cttccgcctt cttcttgccc gcttcattgg ccacccagcc ggagagcacc
    32281 acctttcccg gctccggcac cgtcaccgat acggaaacgc tgtggccgtg gtcaaaagcg
    32341 ggacctgcgt ccacgatcgc agcctgcagg cggcttgtca atgccaggac ctcgatggcc
    32401 ttttgacact cgatgccggc ggtccggatg tcgctggatc gagccaagtt taccacggtt
    32461 tccaccgcca tgtcggtgct cagcttatcc atgttgagca ccaggtcgta gtacctggcg
    32521 tcgtcccaat tcaccccata cacaaacttg atgaacgcgt cccgttcccg gtcggtcttc
    32581 tgcagaatct ggtccgcctc ctctttcctg tagccttccc tcaggaggtt ttccacgcgc
    32641 ttttcccggg aggccacgat ccgcacacgc agggcacagt tgaggtcttt caagaggatg
    32701 gagcttccgc ggcccaggaa aaccgcatcc cccctcttgg ccagttcata gatgacggaa
    32761 tgcaaccgga aaaacccaga gctcaccttg tcggaaaaga gctgctgaaa aaaggagggc
    32821 ggtttctcgt ccaccttctt cacttcctgc aggaaaccca gctcctccgc cgcctggttg
    32881 atggcctccg tgtcgaagta gtgacagttg agtctctccg ccaccctctt ggcgatttcc
    32941 gtgccccccg atgcgaattc gcgagaaaac gtgatgaata gcatggcttg cctcccttgt
    33001 gatcgcctgt gccgcagtcc cgcaaagaac cttccgggat gaccaagaaa cgccccctcg
    33061 ctgaaagagg tctgctcccg ggatcgttgg tcgccgcgcc cgggacgcct cctcctccat
    33121 gaaatgggtt acgctggagc cggggtcttg tcaacgagaa ttacggaagc aatccctgga
    33181 gatgggtagg ggagactccg ccgttggttc gagtgagtcc ggaaagggca atgcagccct
    33241 tccattccgg agagggccgg tgtcgacgat ccaaagtccc ccctccccgc tggaatgagc
    33301 cgggaagccg gggggcgggt gcccttgaaa acccggattt ccccctgcgc cgggatgacg
    33361 aacgcggctt gagtccggcg gcattgcgaa agaaaggcag cgatccgcat aaacggcgtc
    33421 gttagcagga atggttgcac atgcgaccag gcgatggcaa taggcgaaag accgaacttc
    33481 cccaacgcag agcccgccgt caaaatggtc aaggtcaagg acggccggaa ccgcttgcct
    33541 ctcgggggaa gagcggattt gccctccccc tcgccccaag gcaaacccgg gagacttttg
    33601 gatcgaggcc gccctgcccc tgcgaggcgg catctcgggg tttcgacggc agcaggaact
    33661 tggccgacac atccgcttta cccggcaaag gctcgaaatc cgcgggcgtg cagtctcacc
    33721 tattccgccg cccacaggaa cggccacgcg gtgtccaagt ccgcgggcgt acagtctccc
    33781 caacgaacgt cccaacaagg aggtcctcat gaagaagtcg gcttttctcc tggtagtccc
    33841 ggctcttgtc ttcctgttcg caaaagacgc tctttgtgtg ggagccatcg ccgtcgacga
    33901 tgagatcggg gaaagcgaac ccggctacgg gtttgtgacg ggcgcattct ccagggagga
    33961 agccaagcgc cgggccctgg acgaatgcgg aaggagcggg aatgaggatt gccgggtggt
    34021 cctctggttt gaaagttgcg gagcctacgc ggcatccttg acccattacg gcgtcggatg
    34081 gggctccacc aaagagcagg ccaagaagag ggccctggac gaatgcggac ccggatgtgt
    34141 cgtcaaggtt gccgagtgcg aatagacggg tcgggtcgtg aaacggaaga gcttggcggc
    34201 ttgcccgagg ttcctacggt ttgcacatcc cgcacggctt gtagcccgcc ttcaccgctt
    34261 cctcccggct cttgagaacc gccgtgcagt tcttgcagtt gtactgctga caaccgggct
    34321 tgtggaagac acggcttttc acgttgccgt ggtaggttag ggcaggctgg gattgagccc
    34381 ctgcagcggg ggcctcgggg gagacggcgg cggacggagc ctggggcgcg gcggcttgtc
    34441 cccatgcctg tgcaacccag gggagcacaa ccaggacgga aaccaggacc aggaaacggg
    34501 tgagcttctt gttgcggttc ataaccggag cctccttggt gatacaattc ttgtgcaatt
    34561 cggatcggag cggcttgtgc aacgaccgac ggcagccgac agcggataca gcatgccaaa
    34621 ggaaaggggg attttcaatg ggaatttccc ccggacccaa aggctttcac cgctcttgag
    34681 cccctcccga atttgcatag tgccgaagcc cggtcccccc ccaacagggc gggcggttcc
    34741 cgcaactcga cgacccgttg cccatcatcc ggccgtccaa gtgcgtttcg gcttgtttgg
    34801 ggaaaggcgg ggattgtgtc gataaagttg cgccggccat gaatcaggca gtttgcgagg
    34861 atggttttaa cggtccccgg caaattcctg ctcagcgcat cacagatccg gatgggcggc
    34921 ggcagctgag caagcctgcg ggagaggaga tcttcaaggt tttccccggc aatgggccgc
    34981 tcacccatga ggatcaggtg cttgagatgt tctcccaccg gttggcggta accgaggtaa
    35041 tgatggtgtt cgaccagggc attgaagagt ctctcttggg gcagtcttcg cacctgccgg
    35101 atgacaagct ccggcaagcg gcaaagatcg acctcgatgg gggtctgatc gaggctcggg
    35161 cgatcaggtt tctttcgccg gaccaaagga ttgttcggag aacagcgctt ggggggaagc
    35221 tggatttggc cggcccgctc cagttgcagc aagagcgacc ggcagaccat atcgcgcaac
    35281 tcaccgttgg cctggaccca gttccatgcc tgacagagcc tcttggagag ctgtcggcgg
    35341 ctgtcggccg gatgctccgt gatgaggctt tggatgaggg cgatatcctc atccgtaacg
    35401 attcggtttc ggtacttcag ctttgcgtcc acaccccggc caataggacg ggagggactg
    35461 gaacgtaagc tcgaagtgat ctatccgggc aattttttga agaaccgcac cttccttgcc
    35521 ggcaccttga agttccagat gagggcttgc aactcatgag cgtccaaatg ggaacgctcg
    35581 ggccagtggt gaaagcggcc gatggagagg cgcttatgag cgatccagaa gccctgcccg
    35641 tcgtaggtca ggattctcag ggcggacctt tgccggctgc aaaagacaaa aagcgcacca
    35701 tcaaaggggt cttgcccgaa gacctgacgg cagatccggc agagaccgtc gattcctttg
    35761 cggaaatcaa ccggctccac gcagaggaag atgcgcatct ggggaatgat ccggatcatc
    35821 gtcgttgccc gagaaaacag ctgaccaagg acctcagttg cggctggagt cgctcgacgt
    35881 tgcggatctt cagctcaaag cccgtagggg agcgaagctc caggacacat ggggctgttg
    35941 accaaagctg ccgcatgtcg atctcgacaa actgtgccgg atgctcttca gagagacgct
    36001 ttccgatacg gtgcttcagt tcagtgtagt tgagtctcag agccttcgca atccggtggg
    36061 tggaacagca gggcgcaaga tcgacggcgg cctgccacaa ctcttcggga atgcggctgc
    36121 gtggcttgcc gttcctgcgc cagtgggcga aaatcgctga gacttcctgc aagcgggatt
    36181 gcgtttctaa cgggtacttg attgccatcg gccgtcctcc tctcccggaa tagtcagccg
    36241 atcatacccc caagcccccg gccgtttcac gcacccttgc cggaaacaca cgaaaaaccg
    36301 ccgccgcccc ccgccatccc ccgtgggaga tctccccgcg gggcgcaagg accgtctttc
    36361 ccgaaaggac cccgcggcgg atcgtttctc gaagctcctc gtctacggta agaagggaga
    36421 gggcctcgag gttcgaggcc gtgatccggt ggacggggac ctgctgctcg tttgcgtcca
    36481 aaaggacctg cacggcggag acggccgttc ccgggggccg aagaaagaag gcgtcccagg
    36541 cggccccttc caggtaagag tcctggattc ccgttagaag aagaaagata aaccggtcct
    36601 ccggacggtc ggcccccacg gcatagagcc cccggcccac gtccattcgt cgtcccgggt
    36661 agtttcccga aaaaggcgtt cccaggaaat acccgaccga aagaggggcg gaaaaaagcc
    36721 ccaccgaagg aagcctttcc tttctcaccc ccagggaccg ggcccaaagc tcatccaaaa
    36781 gatcgcactg catgaagtag tgtaaaaaga ccccgtaaag gttttccgcg ggggtcgcct
    36841 cgggaacggt ttcccggcga tgctgcaccc tgcggggaga aatcccggcc ccgtttatcc
    36901 cgaagaccag ctcgtctccc gccgaagcgg ggaaatactc cgttgccgtc gtgcggcccg
    36961 cggggtccca aagggtcacg gcccacacct gggaggaccc catgggggcg tcgggtcccc
    37021 gggcgacggt ttccccgtcc aggtgcaccc ggggaaagac tttcaccagg tacaggggaa
    37081 gggaggaggc ccgggcggtt tccaggacct gccgatccgc ggccgtggaa ggttcgaagg
    37141 tcgctcccag cctttttccc gaaagctcgg caaggctcgc ggaaaaggat acaagggttt
    37201 ctcccgtatc cgggtccttg agatcgaggc ccgccctgtg gcgcagggaa tcgggaaggg
    37261 ccgggtaccg ggcccccacg gccgcgacct tgtagggcgg ggtccccagc aggcagggat
    37321 cgtttctttc cacaatcctt ctttttccca ggacttcctt taggagggcg tccggccccc
    37381 gcccggcaaa gtgttttcga atcctttccc ggtagaggcc aagggccggg ccgatctccc
    37441 cggttttgag gcccgcggcc cagccttcct cctcgttgtg cagggcttcc ccgagaaacc
    37501 ggtccatgag agaacccaag tccgggggaa ggtcttgggt gagatcggcg cccgaggtga
    37561 gactcatttc cttgtaggcc gcatccaggg
//
LOCUS       DAOOVC010000024        37106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_728_length_37106_cov_6.525816, whole genome shotgun sequence.
ACCESSION   DAOOVC010000024 DAOOVC010000000
VERSION     DAOOVC010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 37106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37106
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_728_length_37106_cov_6.525816"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2669
                     /locus_tag="PLM79_05180"
     CDS             <1..2669
                     /locus_tag="PLM79_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85734.1"
                     /translation="FQTFDPTPMDTYLYADHPNVVTAMEFERLLSAGGPTTGHLKRPS
                     DLALEGELAELEKELARGVRQLKQLEEKRGKSTGEGLEGALKGGAIDPDTDRRAALAK
                     QVQEGEERLAVLRAKKAAARDPKKIAWLQCVGSRDIHHCGNGYCSGVCCMYSIKEAVI
                     AREHAKGDLDAAIFFMDMRTYGKEFEQYCNRAKESGVRFLRSRIHSVEPVPGTDDLRL
                     SFATEAGQMVEEDFDMVVLAVGLEASPDALELAGKLGIDLDHYRFARTSSFSPVATSR
                     PGVYVCGVLQGPKDIPLSVMEASAAAGAAASRLASSRNTLVKEKTFPAERDVTSESPR
                     VGVFVCNCGVNISSVVRVPEVVEYAKSLPNVVYVQENLFSCSQDAQDRLVQVIREQDL
                     NRVVVAACSPRTHEPLFQETLRNSGLNKYLFEQANIRDQCSWVHSSDPDAATEKAKDL
                     VRMAAARASLIEPLPMPSVQVTPAALVVGGGVAGMVCALTLAEQGFTAHIVEEKDRLG
                     GHARKVKKTWKGEDVGVFLDRLIDTVQGHPNIRLHLNSKVKEVSGFLGNFTTTLSSGG
                     SESTVEHGATILASGAHSSKPDEYLLGKSPRVFRWHELDEAWESDTVKNARSAVFIQC
                     VGSREPQRPHCSKICCTFSVQKALELKERNPETDVYVLYRDIRTYGEREDLYREARGK
                     GVVFIRFDLSRKPQVTQTADGKLQVEVVDHVLGRPVILEPDFITLATGIETRGAEDLA
                     QLFKVPLSQDRFFLEAHMKLRPVDFATDGIFVCGLAHYPKPIEESIAQAQAAAARAAT
                     VLARRSIEVEGVVSTVDSSLCRGCGKCVEVCPYGAPQLLQVKEDVSVSQIQEALCKGC
                     GACAVACPTGAAMIRHFNDREVLTMVEAALCK"
     gene            2790..3209
                     /locus_tag="PLM79_05185"
     CDS             2790..3209
                     /locus_tag="PLM79_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2991..2993,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85735.1"
                     /translation="MSVEFEPRIIAFCCNWCAYAGADLAGVSRLQYPPNIRVIRVMCS
                     GRISPDFILRAFQLGADGVLVSGUHIGDCHYQDGNEKARRMVEMTRELLALMGVDHER
                     LALEWVSSAEAARFAEMVTSFTEKVRKLGKSLLGVAA"
     gene            3206..4333
                     /locus_tag="PLM79_05190"
     CDS             3206..4333
                     /locus_tag="PLM79_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV85736.1"
                     /translation="MNIDEAVKKYQTYYCLDCGVCTGSCPVSRVMSTFSPRIIVEKAL
                     LELGEELLRDRELWSCLTCSRCYERCPTKINFPEFMRVLREEATKQGTTPVLSHHGML
                     QTIMEMQCQGIRQNKTAWAREAGRIAQQGDTFFFVGCMPYFEVIFRDIGVDSLSISRK
                     VIRVLNRAGIVPVVSNEEACCGHDMLWNGRFDMFQKLAARNIELIRQSGAKRVVFSCP
                     EGYYTFLHHYPQYFGDLGFRVMHFYDLVEEMLADGALKIENGDGVITYHDPCRLGRMA
                     GIYESPRKILRKLPDSRFVEMERNRENAVCCGTSGWANCSTCSKQIQVERLREAEATG
                     AKTLVTACPKCQIHLNCALGNMNLDLKIKDLITLIADGMSG"
     gene            4410..4577
                     /locus_tag="PLM79_05195"
     CDS             4410..4577
                     /locus_tag="PLM79_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85737.1"
                     /translation="MSNDVLVVGGGIAGIQAALDLAEMGIRVHMVERLPSIGGKMAQL
                     DKTFPTNDCAI"
     gene            4596..5957
                     /locus_tag="PLM79_05200"
     CDS             4596..5957
                     /locus_tag="PLM79_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85738.1"
                     /translation="MDVGRHPRINLLAYSELEEVSGEMGAFRVKVRRKARYVNENLCT
                     GCGSCAEKCPTLVVDLYNAGLGKRKAIYKYFPQGIPSVFTIDAGKCRQLGEGKKCGVC
                     AKVCQAKAVDFEQKDRMVELEVGAVILATGYEVFDPSGIPEYGYDRIANVVTALEFER
                     LLSASGPTAGHLDRPSELALEADIEELEKDVKKLGRQVKALEDKHGKSSTQILDRVKS
                     GTLTDQESLAWAATAEKLAEAEADLEKKKQKKAAAGTAKKLAFIQCVGSRDFRYNRFC
                     SSYCCMHSVKEAMIAHEHDNEVSSSIFCMDLRAVGRGFEEYKLRGGKQAGIRYVRGRV
                     AEITEDSAGNPIVWYESTTSRQVLHETFDMVILATACVPSASTAELARLFGVEVDENG
                     FFRTDPLLPLNTTRPGIFVCGCAQGPMDIPESVAQASSAAASAAEVVASPPGEAAQAY
                     RYA"
     gene            6031..7704
                     /locus_tag="PLM79_05205"
     CDS             6031..7704
                     /locus_tag="PLM79_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV85739.1"
                     /translation="MAEEIRIGVFVCDCGSNIAGYLDVKALALYAETLPNVVYVKENL
                     YTCSESGINEIKAAIGEQNLNRVVVASCTPRTHEPLFRATCAEAGLNPYLFEMVNIRD
                     QCSWVHMQEREDGTAKAKDLIRMGVAKAALLEPQEPIQSEMDAKAMVLGGGISGMTAA
                     LALANRGYQVFLLEKTPRLGGLLLRLSRLGPSNADASELAAKYAERVQKHPLIQVMTS
                     TVAEEVKGYIGHFQVTAVRNGNREEFGVGVIIVAIGAEVLQPHGLFQYDGKSVINQLE
                     LEERLKSEDFSAGSVVMIQCAGARNEDRKYCSRICCMVAIKNAIALKERNPDVGVRVL
                     YRDIQAYGTENEAMFQRAKELGVLFLKYDPAMPPRVEPGKVVVFHETLGRDLTLPAEL
                     VVLSTPLIAGPDNETVSKLLRVSLDENKFFLEGHVKLKPLDFATDGVYLCGNAHYPAT
                     VREAVSQALGAASRASIPLSKKVMTVEPIVSTLVDEDACRGCGLCVALCPYGALEVVR
                     TEKGRKVRVIPVACKGCGVCAATCYRHALSVNSYSDQQIGEQIRAFLGE"
     gene            7843..8568
                     /locus_tag="PLM79_05210"
                     /pseudo
     CDS             7843..8568
                     /locus_tag="PLM79_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
     gene            8642..10312
                     /locus_tag="PLM79_05215"
     CDS             8642..10312
                     /locus_tag="PLM79_05215"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85740.1"
                     /translation="MSEKRVIVIGGDPAGVAAALAEAEAGSRVTLVESSPSLGGGRIP
                     QTRILDAGDAFVDPNLEALMRHPNVRVISNARVERVAAENGGYRFRIRRKTPRVDWEK
                     CNDCKACIRVCPIHMYDDFNEGVGFRTAIDFFNPETGQYNIFKEDMPVCQATCPANLD
                     IRSYVGLIADGKYDRSLAKIRERLPFALSIGRVCPHPCETACNRGYMDEPISICTLKR
                     FVADYEMKSGVPAPVEVPDRFHPEKVAIIGAGPAGLTCAYFLAKAGYHSVVFEALPEP
                     GGMFRYGIPEYRLPTSTLMYEINWILSHGAELRCNTRIGKDIDFEEIRKQYDAVFVGV
                     GAHAGMKLQIPGEDLEGVVDGVDFLRDVNGGKKVNAKGKVIIIGGGNVAMDAARVSWR
                     EGFDEVHILYRRTKKEMPASPWEIEAAEHEGIKIQYLVAPIEVIGENGRMKALKCLRM
                     QLGEPDASGRRRPVPIPGSEFEIEAETLIPAIGQRPDLTFVPEQGGPTITRWNTFSVH
                     RDTYMTNVPGVFAAGDAETGPDIAIRACAGGRKAAAGIVRYLESKRQK"
     gene            10326..11213
                     /locus_tag="PLM79_05220"
     CDS             10326..11213
                     /locus_tag="PLM79_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85741.1"
                     /translation="MMYEKKGRYIVEFPDIPKARQHHVEIDVDARRGNYREVELGFPE
                     DRAVLEAKRCLSCRRCLGCALCWAECKPEAIRFEMEDEVFDLDADAVVVSAGVDRPVE
                     RLDRRFGFGTFLNVITDLQLERMLSRSGPSAGLVLRPYDGEIPRSVAFVQSYGSAAPA
                     MHRRALCFGVNEAVLVRQKLADAEIRVYAADLSSFREEFAKDLEKLDGIEIHENHVAK
                     IEATEDQCLRVFFEEDGQTREKTFELVVLLTQPQLSGELKDLSKSLGLSYAYADFLAG
                     NGPGVIRTEKESIGLAAQA"
     assembly_gap    11381..11480
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11549..12751
                     /locus_tag="PLM79_05225"
     CDS             11549..12751
                     /locus_tag="PLM79_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOV85742.1"
                     /translation="MSGSDFFKALGIEEGDRGAVKRFAEVSRVPVDRLRHYHAANILP
                     SGPDRDRVCAAAGISPAELMLRMGVLDGRILAALRSKAREVFELLRDDLEDSSGPLEP
                     PPVVYKTDLGKLHHGDCLALMKHLEDESVDLVFADPPFNLKKLYPSGVNDDLKEAKYL
                     EWCESWAAECVRLLKPGGSLFFWNIPRWNTYLAGYLNARLTFRHWIAVDIKYSLPIRG
                     RLYPSHYSLLYFCKGEKPKTFRPDRLPMEVCPACKADLRDYGGYKDKMNPRGVNLTDV
                     WTDIAPVRHSKYKMHRGSNELPVKLMDRILEMASEEGDLVFDPFGGSGTTYVVAEIKR
                     RRWIGIELGPMDDIVRRLEQIRTDANLVGKIRRGYNRLFTEETLHARKQLGLWTCDSV
                     GKTKGETP"
     gene            12800..13450
                     /locus_tag="PLM79_05230"
     CDS             12800..13450
                     /locus_tag="PLM79_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOV85743.1"
                     /translation="MKLYIDSARVSVIESFMKTGVFHGVTTNPHLLKEAGVPPERLSE
                     LARQVFDLGAGEVFFQSWGEDAAALHLRGRQLADLDDRVVVKLPATLEGFEAASMLAA
                     AGTRTCITAVFAAFQGILAAGAGAAYVAPYLGRMNDAGRDGHAVIALMSGALRRVESP
                     TAILAASVRTVDDVALLAQNGVRCITLSPAVARNLFREPLTLEAVRSFETAANAIE"
     gene            complement(13579..14010)
                     /locus_tag="PLM79_05235"
     CDS             complement(13579..14010)
                     /locus_tag="PLM79_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85744.1"
                     /translation="MKKMNWIALALALTILVSGAPSLYAQQQEPAPQAQQDEWRCPWM
                     GKGPMMGRGPGMGRGFGRGWCMRGGCPMAGINRGEPVTREQAILMLENYVRYTNNPNL
                     KLGDVVDRQDFFEGSIVTKKEGALVEKIQVDKKGGWFRNAS"
     gene            complement(14121..14375)
                     /locus_tag="PLM79_05240"
     CDS             complement(14121..14375)
                     /locus_tag="PLM79_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85745.1"
                     /translation="MKKTITIAALAAQLFFTGLGTTYAQEAKPGDGGTSPEWTCPAYG
                     QGKHWARGAGACRQEAVPACGGPLGGWRTRAHYGPHHFAR"
     gene            14857..16593
                     /locus_tag="PLM79_05245"
     CDS             14857..16593
                     /locus_tag="PLM79_05245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85746.1"
                     /translation="MGFRISNPTRWAWFSPWILLGSVCILAGILLILGVKNIHREKEF
                     VERALLSEANVLIRSIEAGSRTGMMGMGWGRRQHQMLLEETAQQSDVLYVALVNPRGQ
                     VVAHSDPRQVGSVLPLEFPRQGETSYRFLHKEPKSFEVARAYQPWFRQRGRRGGESCE
                     VGSSSGKDMDLYILVGLDPGPLEEAQRQDLQHTLVLFSVMFLVGAAGFVSLVWAHHYR
                     TARCSLKDIQAFTSTLVNQMPVGLVATDLEGRIERANEAARNLLRPGGGSVEGNIREY
                     PSFLPVVQRLGNEQEMVEQEILCGSSGREPVPLLVNAAHIRDGEGRKAGFVFLLADMR
                     NVKQLEEQLRRNERLAGLGRLAAGVAHEIRNPLSSIKGFATILAGRSGEDERSHKIAH
                     AMVQEVERLNRVVTELLDFARPTELHKRPTACRELIENSLRLVEGDAGKQGVRVESRV
                     EPGDLEVELDPDRFSQVLLNLYLNAIEAMETGGTLRVEVLREAQAAVFRITDTGSGIF
                     PESLPHVFDPYFTTKPRGVGLGLANVHKFVEAHGGEVEVESTPGRGTRFAVRLPLFPG
                     RPSGEKRAGETG"
     gene            16643..18037
                     /locus_tag="PLM79_05250"
     CDS             16643..18037
                     /locus_tag="PLM79_05250"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85747.1"
                     /translation="MTKEAGTLVLVVDDDPAHRLMITTLLEEWGYRTRDAEDGKKALD
                     FIRANPVDLILMDMRMPEMDGIEATRQIHGHNPAIRIVIMTAYSSIPTAVEALKAGAF
                     DYVTKPIDFEALRLSMERALEHTRLLQENETLRRQLAQFQIPEILGGSPAMRRLMDMI
                     GLVAPSEATVLITGESGTGKGLVARAIHANSQRRGKSLVEVNCAAIPETLIESELFGH
                     EKGAFTGADRQRRGRFSMADQGTLFLDEVGELPLSMQAKLLSVLQQGHIQRVGSDVSI
                     PVDVRVLAATNRNLPQMIAGGAFREDLYYRLNVVALEVPPLRERIEDIPVLVGHFQAH
                     FAEKNKKIVKGFTPQAMDHLLRYSWPGNVRELENAVERAVILLRGEYITERELPLSLR
                     QPAPGEGTRKEEREGADLPEGESTLAELERQQVLRVLEESGGNKSEAARRLGITRRTL
                     KLKLKKYEEEKGTT"
     gene            18518..19030
                     /locus_tag="PLM79_05255"
     CDS             18518..19030
                     /locus_tag="PLM79_05255"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOV85748.1"
                     /translation="MIERDFDTAFIADLALREKQIQQNYRPIIAVHKWFARRPGTLFR
                     GLLLSEFVNAPLRDSFYRAHDLSGIRIGDPFNGGGTPLIEANRVGCDVVGRDLNPMSY
                     RIVKREIEHLDLDAYRAVGDALVRTLDEQIGRFYRTRCSHCGSRRCPYFCVGLRVSSR
                     NVQFPPSGRP"
     gene            complement(19303..20271)
                     /locus_tag="PLM79_05260"
     CDS             complement(19303..20271)
                     /locus_tag="PLM79_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOV85749.1"
                     /translation="MDIITIAMVASGLFFGWTIGSHYTGATMGMAYGSGIVKRPLTAT
                     ALVALFALLGATFESRHVVETIGTGIIKGTDMTPLGAMTMMFTAAVVTAANTWMRWPV
                     STSQLACFSVVGTAIAMGAPVFWGSTIVFLCVTWVATPLVAAVFGWVFTRATDWTIKG
                     RSDTVQKILGYALFIASCYSSYTLGANNTGNAVGVFFGLKLTTEMQAGFIGGIVIAIG
                     ALTWGVPLLQKVGKDLVQVDLSVGVGAKFAQSVTAHIAAVLGFPTSMNQAQIGGVAGA
                     AYVRGLQTIDMAAMKEIVLSWFLTPVVAGVVSFFLYHLFSFILKIH"
     gene            22364..24574
                     /locus_tag="PLM79_05265"
     CDS             22364..24574
                     /locus_tag="PLM79_05265"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOV85750.1"
                     /translation="MIERDFDTAFIADLALREKQIQQNYRPIIAVHKWFARRPGTLFR
                     GLLLSEFVNAPLRDSFYRAHDLSGIRIGDPFMGGGTPLIEANRVGCHVVGRDINPMSY
                     WIVKREIEHLDLDAYRAVGDTLVRTLDEQIGRFYRTRCSHCGSQDAHVKYFLWVKVMN
                     CSQCREEISLFPGYLVSGDKRHPMNVLVCGECGQLTEVEDGKNPGVCKQCGRSLSRTG
                     PAKRNHCPCPRCGRIHGYPDAGAGPPRHRLFAMEYHCPRCRTRQKGRFFKIPDPEDLA
                     RLDEVESMGKRLQPTFVPDDPIPGGDETNRLHRWGYRYYREMFNSRQLVGLELSCRFI
                     AAQPDERIRNALATNLSDLLRYQNMLCRYDSMALKSLDIFSVHGFPVGLIQCESNLLG
                     IPGRANGENVGSGGWSNIITKFVKAKSYCLQPFEIQHRSGRKVVIEISGEWIGETSRL
                     FPAVKSRGVDLACSDAAVADLEPGSLDAVLTDPPYFGNVQYAELTDFCYVWLRKLAGR
                     GVPEFSTGSTRNPAELTGNENMGRGLEHFTRGLSSVFRKMAIALKPGAPMAFTYHHNR
                     LEAYYPVAVAVLDSGLTCSASLPCPAEMGASIHISGTKSSVVDTVFVCRSTGKIPAKW
                     IVGSPEGLAGLVREDLEKLRGGNFSPTAGDLRCIIYGHLVRLAVWDLRKGWNRDLETA
                     HRLGRVEEWIRAFGGLESVERHLRDVISTIPHVQQAIAQEAQSSYREPPSEVSF"
     gene            24558..25586
                     /locus_tag="PLM79_05270"
     CDS             24558..25586
                     /locus_tag="PLM79_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85751.1"
                     /translation="MKFPFEASLQEIQENPDPFVSAVFSCLESEFLLMPKGSGFIEYP
                     VFECGYEALKKATSGFREFAGEPVYSTVISVPISLIVLRSMLGFTPSEWAYIATQRTG
                     VEVTQGHARTLDRKIRMMPSAPLKVQGEAAARLKALVDSACQMLVEGAPDVEPDKLHR
                     LDKADTRNGIQAVQNMAAMGVPYAMLLYERLLGRPFSSHRDSVSELVGDRLESAIEDL
                     LARAGISYRKTKRAKRIEGFDQAPDFMIPSEFHPQVVIEAKFTEDDGTARDKVTRIQH
                     LAELSSMGVPSGQARFEVIACIGGRGFGVRREDRKKMLLATGGKVFTLKVLDRLVEHS
                     RLKDFRTG"
     gene            25899..27515
                     /gene="glgP"
                     /locus_tag="PLM79_05275"
     CDS             25899..27515
                     /gene="glgP"
                     /locus_tag="PLM79_05275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOV85752.1"
                     /translation="MKVAYFSMEIGLNENIPTYSGGLGILAGDHIKSAADLNVPLVAV
                     TLLYKRGYFVQSINPLGQQEETYPYFDPRAFMEQLPFKVTLKLEGRDLHISVWKYTQV
                     GLTGKVPVYFLDTDLPINAPEDRLITQYLYGGDQHTRICQEKVLGIGGYLALKRLEPG
                     VTTYHMNEGHAAFLTLALLKDMDGNEEKTRDKCIFTTHTPVPAGHDKFHYDMAEEVLG
                     DYLPSNIRSLAGEDLLNTTLLALNLSRAANGVSELHGEISRRMFPGFDIGHITNGVHH
                     LSWTGREFKELFDRHVPTWRQQPQALKGALNIPTDEIREAKLSAKKRLISYVNSATGA
                     GFSHELLTICFARRATAYKRTTLTFKDMEYLTNLSFDRVQFIFAGKAHPQDGAGKELI
                     KEIVNTGRQYEKQLRLVFIPNYTIWLASLMTRGTDVWLNTPRRPREASGTSGMKVCFN
                     GGVNMSVLDGWWREVCRSRINGWAIGDDEDQTDEEAANDLYQDLDDLVTTYYANPKQW
                     LAIMKNSIADVTPVFNTHRMVTEYLRTYYL"
     gene            complement(27594..28805)
                     /gene="argJ"
                     /locus_tag="PLM79_05280"
     CDS             complement(27594..28805)
                     /gene="argJ"
                     /locus_tag="PLM79_05280"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOV85753.1"
                     /translation="MVSRGPFEVPGFRVAAEASGMRYQGRPDLAWICTESVEGAVAAG
                     VFTRNRFTAAPVQLCREHLKSDRICSILVNAGIANACTGDEGLEKAREMARIAGESLG
                     VDPKTVLVASTGIIGQQVRLGPVRQSISTLRNTLRPDAWEEVARAIMTTDTQPKMAST
                     RILLGGREVTVGGVAKGSGMIAPDMATLLAFVCTDAAVGKGVLEHWVRAGADRSFNRI
                     TVDGDTSTNDTLLVLAGGAAGNPVLSALDSPDSRLFGEALEAVLLDLARRIVLDGEGA
                     TKFIELHVTGAPDAESARRVAFTVAHSPLVKTAFFGEDANWGRIVAAAGRADVPLDPQ
                     QVTLHFGDVCVFKGGKPIEGVDVEEKAAGVFRQKEIPVHLDLGMGGFDFVAYTCDLSY
                     DYVKINASYRS"
     gene            complement(28795..31320)
                     /gene="secA"
                     /locus_tag="PLM79_05285"
     CDS             complement(28795..31320)
                     /gene="secA"
                     /locus_tag="PLM79_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOV85754.1"
                     /translation="MIGALLKKIFGSHNERTLKKIAPLVDEVNRFEPQVRKLSDAQLR
                     AKTPEFRQRLGNGEPLDDLLPEAFAVAREASVRTLGMRPFDVQLIGGIVLHQGMIAEM
                     KTGEGKTLVAALPIYLNALTGRGVHLITVNDYLARRDSEWMGKIYRFLGLSVGCIVHG
                     LDDRERQEAYGADVTYGTNNEFGFDYLRDNMKFRLEELVQKDLHYAIVDEVDSILIDE
                     ARTPLIISGPAEKSTELYYRINRIIPQLHKEAHYTIEEKSRTVALNEEGVARVENLLG
                     VENLYDPRHITILHHVHQALRAHALFKRDVDYIVKGGKVIIVDEFTGRLMPGRRYSEG
                     LHQALEAKENVRIENENQTLASITFQNYFRMYEKLAGMTGTAETEAAEFAKIYKLDVV
                     VIPTHRKMIRTDHPDCIYRTEKEKFRAVTREIKELHRMGRPVLVGTINIEKSEKLSEM
                     LKREGVPHQVLNAKHHEKEAEIVALAGQPGAVTISTNMAGRGTDIVLGEGVAKLGGLH
                     ITGTERHEARRIDNQLRGRAGRQGDPGSSRFYLSLEDDLMRIFAADRISGLMQRIGME
                     EDEPIEHRLISRAIENAQSRVEAQNFSIRKHLLEFDDVMNQQREVIYRQRREALRGES
                     LKPAIMDMVEDLVEGLITDCTSEKTYAEDWDLETLNKELSRLFGMQATFTVESLEDVS
                     QEQLRESLLEQVVQQYEAREKEFGEAVMRDLENYLLLQTVDTYWKDHLLNMDHLKEGI
                     GLRGYGQQDPLIAYKREGHQLFQEMIQRIKEETVRLLYHIQIQHEDQVQDLKREQEEQ
                     PMFYGPGDGGSSRPAAARKDKKVGRNDPCPCGSGRKYKKCCGK"
     gene            complement(31798..32259)
                     /locus_tag="PLM79_05290"
     CDS             complement(31798..32259)
                     /locus_tag="PLM79_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOV85755.1"
                     /translation="MIRKAKTQDVVVVQKILAHFAQQERLLARSLSELYTNLRDIFLA
                     EDDETGQVVGCCSLHIVWEDLAEIRSLAVLDSHQKLGLGRKLVNACVEDARSVGIRRV
                     FTLTYETGFFERLGFRLVDKNIFPHKIWADCLHCPKFPECDEVAMVLDLAG"
     gene            complement(32296..33438)
                     /locus_tag="PLM79_05295"
     CDS             complement(32296..33438)
                     /locus_tag="PLM79_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOV85756.1"
                     /translation="MSIYLDNAATTFPKPPAVYAAVQHAMAEVGANPGRASHRRAREA
                     LAMVSDTRRRTAEILGIPDPDRVAFTKSATESINVVLKGWLRPGDKVLTSGLEHNSVV
                     RPLARLETLGVRTEIIPCSRRGELDLEGFARALEGGLRLVVLTHASNVNGALLPIPRI
                     ARMCRESGVPLFLDAAQTAGVQPISAGEWDLGMLACSGHKGLLGPAGVGVLYVREDLD
                     VLPLVEGGTGSRSEDPRQPGFCPDRYESGTLNFPGIAGLGAGIRYLLEEGLETVLNHE
                     LKLAAEIEDGLSELNGVEVLRPSVRGTGVVSFTVEGMSPDEVAFLLDKAFDIAVRSGL
                     HCAPQAHRTLGTFPSGTVRVSPGHMNTPEQIDSFLRSVHTILQHRR"
     gene            complement(33543..34412)
                     /gene="fdhD"
                     /locus_tag="PLM79_05300"
     CDS             complement(33543..34412)
                     /gene="fdhD"
                     /locus_tag="PLM79_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOV85757.1"
                     /translation="MVPKEFLRDQELPDKDVSRDEDEETAPVSTWVFDKNQEGVLRRQ
                     WIVKEQPVTLYLNGEEMVTLLCAGHHLDELAIGFLHAEGFLKEPQDLERIEIDGELGK
                     VEAFVPGASPLLRNLWEKRTITSGCGKGSVFYYALDSLLAQKIQSPLRVSPRQVLDRI
                     DDLNRLSETYRRTHGVHNTALASPEEILLFRNDIGRHNAVDMIVGHVFQKRISLEDKF
                     ILTTGRLTSEILIKAAKVGLPMLVSRNTATTLAVELAGSLNITLIGYARGGKFTVYSG
                     EERVVGGELGLLG"
     gene            complement(34416..34922)
                     /locus_tag="PLM79_05305"
     CDS             complement(34416..34922)
                     /locus_tag="PLM79_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV85758.1"
                     /translation="MPVLHGIQCPRCGTQVTTYRNPLLTVDILIRTATANDLVLIRRK
                     NPPPGWALPGGFVDYGETLEEAARREAKEETGLDVLDLRQFRAYSDPARDPRHHTVSV
                     VFTAVGRGTPAAADDASQLGIFSADNLPHPLAFDHARIVDDYFRWISRGRLEGPPLPE
                     ADSDIPQE"
     gene            complement(34930..36231)
                     /locus_tag="PLM79_05310"
     CDS             complement(34930..36231)
                     /locus_tag="PLM79_05310"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOV85759.1"
                     /translation="MDKPQYVYGIDVPPPLRYVLLYGLQWAIVIFPALIIVATLGSRA
                     LRLPVDQEVHFFQLLLLLSGLFTVIQTLWGHRYPILDGPSTALLLTFIVLAPYGAEII
                     QGGTIAGGILLMALVFSGKLHRLLAYATPNVVGVILMLIAFSLLPHLMASVTGKDALQ
                     PNGSAGILALSFLLVLAMAAFSHWIEGFWKTLSLLLGMLLGTLLFFLLEPLDSSPLGA
                     APWVSLPSMRFLTPPRFYWPAVAAMAASYLAVVVNTLGSLHGVAAITDTERLPTAVTR
                     GVFWNGAAGICCGTLGIVGLVSYSMSPGVILANRVASRYAVACGGILLVVAAFVPKLA
                     AVLAMIPAPVVGAALCVAMGAQVGAAMGIVAEGDLSGRDYFVVGVPVLMGTLVSFLPS
                     SLLESIPIHFRVFLGNGLVVGIFLVLFLEHFLLRKKRPREE"
     gene            complement(36258..>37106)
                     /locus_tag="PLM79_05315"
     CDS             complement(36258..>37106)
                     /locus_tag="PLM79_05315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOV85760.1"
                     /translation="TFSDGEIRIEIQENVRGADIFVVQSGAHPVNDHLMELLVMIDAM
                     KRASARRITAVIPYYSYARQDRKNKPRVPITARLVADLITRAGAHRILTMDLHAGQIQ
                     GFFDIPVDNLYASPILLPYIREHFNSNLVIVSPDAGGVPRARAYARRIQAGLAMIDKR
                     RSDVNEAEALNIIGEVDGKTAVVLDDMVDTAGTLVEATRTLLEKGAREVHACVTHAVL
                     SGPAVERIEKSALKSLVVTDTLPLRPQAAHCQKIVVVPSFKLFSAAIRNIHNEDSIST
                     LFEIQH"
BASE COUNT         7314 a        10975 c        11541 g         7176 t
ORIGIN      
        1 gcttccagac cttcgatccc actcccatgg atacctacct ctacgcggat catcccaacg
       61 tcgtcacggc catggaattc gaacggctgc tcagcgcggg aggtcccacc acgggccacc
      121 tgaaacggcc ttcggacctc gccctcgaag gggaactcgc ggagctggaa aaggagctgg
      181 ccagaggggt ccgtcagctc aagcaattgg aggaaaagcg ggggaagagc accggcgagg
      241 ggctggaagg ggccttgaaa ggaggggcga tcgatcccga cacggatcgc cgggcggctt
      301 tggccaagca ggtacaggaa ggggaggaac ggcttgccgt cctccgggcc aagaaggccg
      361 ccgctcggga ccccaaaaag atcgcctggc tccaatgcgt gggatcccgg gacatccacc
      421 attgcggtaa cgggtattgc tccggcgtct gctgcatgta ttccatcaag gaagccgtca
      481 ttgcccggga gcatgccaag ggggacctgg atgccgccat cttcttcatg gacatgcgca
      541 cgtacggcaa ggaattcgag cagtactgca atcgagccaa ggaaagcggc gttcgtttcc
      601 ttcgatcccg gattcacagc gtcgagccgg tgcccggcac cgacgatctg cgcctgagct
      661 ttgccacgga ggccgggcaa atggtcgagg aagatttcga catggtggtc ctggccgtgg
      721 gtctcgaggc gtcccccgat gccctggaac ttgccgggaa actgggcatc gacctggatc
      781 attaccggtt tgcccggacc agcagtttca gtcccgtggc cacttcccgg ccgggcgttt
      841 acgtttgcgg cgtgctccag ggtcccaagg acattcccct ctccgtgatg gaggcgtccg
      901 cagcggccgg agccgcggcc agcaggctgg cttcctcccg aaacaccctg gtcaaggaaa
      961 agacctttcc cgccgagcgg gatgtgacct cggaatcccc gcgggtcggc gtctttgttt
     1021 gcaactgcgg cgtgaatatt tcgagcgtgg ttcgagtgcc cgaggtggtg gaatacgcca
     1081 agagccttcc caacgtggtg tacgtccagg agaatctgtt ctcctgttcc caggacgccc
     1141 aggaccgcct ggtgcaggtc atccgggagc aggatctcaa ccgggtggtg gtggcggcct
     1201 gttctccgcg cacccacgag cctctcttcc aggaaactct ccgcaacagc ggtctgaaca
     1261 agtacctttt cgaacaggcc aacattcggg atcagtgttc ctgggtccat tcgtcggacc
     1321 cggacgccgc cacggaaaaa gccaaggacc tggttcgcat ggccgcggcc cgggcgtccc
     1381 tcatcgagcc gcttcccatg ccgtccgtgc aggtgactcc cgccgccctg gtggtgggag
     1441 ggggagtggc ggggatggtg tgcgccctga ccctggccga acagggattt acggcccaca
     1501 tcgtcgagga gaaagaccgg ttgggcgggc atgcccggaa agtcaagaag acctggaagg
     1561 gggaggacgt cggggtcttt ctcgaccgat tgatcgacac cgtccagggc catcccaaca
     1621 tccggttgca cctcaattcc aaagtgaagg aagtttccgg tttcctgggg aattttacca
     1681 ccaccctgtc ctcgggcggg tcggagtcca ccgtcgagca cggggcaacg atcctcgcct
     1741 ccggggctca ttcgagcaag cccgacgagt acctgttggg gaaaagcccg agggtgtttc
     1801 ggtggcatga actggacgag gcgtgggaga gcgatacggt caagaatgcc cggagcgccg
     1861 tgttcatcca gtgcgtgggt tcccgggagc cccagcggcc gcattgcagc aagatctgct
     1921 gcaccttctc cgtgcagaag gctctcgagt tgaaggaacg caatcccgag acggacgtct
     1981 acgtcctcta ccgggatatt cggacctacg gggaacggga agacctgtat cgggaggccc
     2041 gtgggaaggg agtggttttc attcgtttcg acctttcccg gaagccgcag gtgacccaga
     2101 ccgccgacgg aaagttgcag gtcgaggtgg tcgatcacgt tctgggaagg ccggtgattc
     2161 tcgaacccga cttcatcacc ctggccaccg gcatcgaaac ccggggcgcc gaagacctgg
     2221 cgcagctctt caaggtcccc ctcagccagg accggttctt cctggaagca catatgaaac
     2281 tgagacccgt ggattttgcc acggacggca tttttgtttg cggtcttgcc cattatccca
     2341 agcccatcga ggaatccatc gcccaggctc aggcggctgc cgcccgggcg gccaccgtcc
     2401 tggcccgcag gagcatcgag gtggaagggg tggtttccac ggtggattcg agtctgtgcc
     2461 gcggttgcgg caagtgtgtc gaggtttgtc cctacggagc gccccagttg ctgcaggtaa
     2521 aagaggacgt ttccgtgtcc cagatccagg aagccctctg caagggctgc ggtgcctgtg
     2581 ccgtggcctg tcccacgggg gccgccatga tccgccactt caacgaccgg gaggtcctga
     2641 ccatggtgga agcggccttg tgcaagtaag gtccgcccgc gaacgggacg agggggaatc
     2701 ttccttacgg acaagctctt agcccgggcc ggagtgtcgg cgttcctcct gccgggatga
     2761 tcgttcatct gcgtgttaag gaaaaggaca tgagcgtgga gttcgagccg agaatcatcg
     2821 ctttttgctg caactggtgt gcttatgcgg gggcggacct ggcgggggtg agccgcctgc
     2881 agtatccccc caacatccgg gtaatccggg tgatgtgttc cggaaggatt tccccggact
     2941 tcatcctcag ggccttccaa ctgggggccg acggcgtcct ggtgtccggg tgacacatcg
     3001 gagactgcca ttaccaggat ggtaatgaaa aggcccgacg catggtggag atgactcggg
     3061 aactcctcgc cctcatggga gtcgaccacg aaaggctggc cctcgagtgg gtgtcttcgg
     3121 ccgaggcggc ccgctttgcc gaaatggtca cctcgtttac ggaaaaggtc aggaaactgg
     3181 ggaaatctct ccttggagtc gcggcatgaa tatcgacgaa gcggtaaaga agtatcaaac
     3241 ctattactgt ctggactgcg gggtgtgcac gggcagctgc cccgtgtccc gggtcatgtc
     3301 caccttttcc ccccgcatca tcgtggaaaa ggcccttctt gaactggggg aagaactgct
     3361 ccgggatcga gagctctggt cctgcctgac ctgcagtcgc tgctacgagc ggtgcccgac
     3421 caagatcaac ttcccggaat tcatgcgggt gctccgggaa gaagccacca agcagggcac
     3481 cacccccgtt ctctcccacc acggaatgct ccagaccatc atggaaatgc agtgccaggg
     3541 aattcggcag aacaagacgg cctgggcccg ggaggccgga cggattgccc agcagggcga
     3601 cacgttcttc ttcgtgggct gcatgcccta tttcgaggtg atcttccggg atatcggcgt
     3661 ggacagcctc tccatcagcc gaaaagtgat ccgggtgctc aaccgggcgg ggatcgttcc
     3721 cgtggtgagc aatgaagaag cctgctgcgg acacgacatg ctctggaacg gccggtttga
     3781 catgtttcag aagctggccg cccgcaacat cgagctgatc cggcaatccg gggccaagcg
     3841 ggtcgtcttc agttgccccg aaggttatta tacgttcctg caccactatc cccagtactt
     3901 cggggacctg ggcttccggg tgatgcactt ctacgacctg gtggaagaaa tgctggccga
     3961 cggagccctc aagatcgaga acggggacgg ggtcatcacc taccacgacc cgtgccgcct
     4021 gggacgcatg gcgggaatct acgagagccc tcgaaaaatc ctccggaagc ttcccgacag
     4081 tcggttcgtg gagatggagc gcaatcgtga gaacgccgtc tgctgcggaa cgagcgggtg
     4141 ggccaactgt tcgacctgtt ccaagcagat ccaggtggaa cggctgcggg aggccgaggc
     4201 cacgggggcc aagaccctgg tgaccgcgtg ccccaagtgc cagatccacc tcaattgcgc
     4261 cttgggcaac atgaacctgg atttgaaaat caaggacctc atcactctca ttgccgacgg
     4321 aatgtccggt taggggacgg aggcgtgagg ccttcctcca tcccgggcgt gatgggcgac
     4381 tcgaaaacga aacacggacg aggtgactag tgagcaacga tgtattggtg gtcggaggcg
     4441 gcatagccgg gatccaggct gccctggatc tggccgagat gggcatccgg gtgcacatgg
     4501 tggaacggct ccccagtatc ggcgggaaaa tggcccagtt ggacaagacg tttcccacca
     4561 acgactgcgc catctgaatt ctctcaccga agttgttgga tgtcggtcga catccacgaa
     4621 tcaacctcct tgcctacagc gaactggaag aagtcagcgg cgagatgggg gcctttcggg
     4681 tgaaggtgcg gcgcaaggcc cggtacgtga acgagaacct ctgcacgggc tgcggttcct
     4741 gcgccgagaa atgcccgacc ctggtggtgg acctctacaa cgccggcctg ggcaagcgta
     4801 aggccatcta caagtacttc ccccagggga ttccctcggt tttcaccatc gacgccggga
     4861 aatgccgcca gttgggggaa ggaaagaaat gcggggtgtg cgccaaggtt tgccaggcca
     4921 aggcggtgga tttcgagcaa aaagaccgga tggtcgagct cgaggtcggg gccgtcatcc
     4981 tggccacggg ctatgaagtg ttcgatcctt cgggaatccc cgaatacggc tacgaccgga
     5041 tcgccaacgt ggtcacggcc ctcgagttcg aacggctgct cagcgccagc ggacccacgg
     5101 ccggacacct ggatcgaccc tcggaactgg ccctcgaggc ggacatcgaa gagctcgaaa
     5161 aggacgtgaa aaaactgggc cgccaggtga aagccctgga ggacaaacac gggaagagtt
     5221 cgacccagat ccttgaccgg gtgaagtccg gaaccctcac cgaccaggaa tccctggcgt
     5281 gggccgcgac ggcggaaaag ctggccgagg ccgaagcgga cctcgaaaag aaaaagcaga
     5341 agaaggcggc ggccgggacg gccaaaaagc tggcattcat ccagtgcgtg ggatcccggg
     5401 attttcgcta caaccgcttc tgctcgtcct actgctgcat gcactcggtc aaggaagcca
     5461 tgattgccca cgagcacgac aacgaggtga gttcctccat cttttgcatg gaccttcggg
     5521 cggtgggccg gggcttcgag gaatacaagc tccggggagg aaaacaggca ggaatccgct
     5581 acgtccgggg gcgagtggcc gagatcaccg aagactcggc cgggaatccc atcgtctggt
     5641 acgaatccac caccagtcgg caggtgcttc acgaaacctt cgacatggtg atcctggcca
     5701 ccgcctgtgt tccgtcggcg agcaccgccg aactcgcccg gctcttcggg gtggaagtgg
     5761 acgagaacgg gttcttccgg acggaccccc tccttcccct gaacaccacc cggccgggga
     5821 tcttcgtgtg cggatgcgcc cagggtccca tggatatccc cgaatcggtg gcccaggcga
     5881 gcagtgccgc cgccagcgcc gccgaagtgg tcgcatctcc cccgggagag gccgcccagg
     5941 cgtatcgata tgcatgaggc gggaggcgga ttcgggactt ttgccgaccc tttcctcccg
     6001 tgaaccgggc ctgaatcaag aggtgctaaa ttggctgagg aaatccgtat tggagtgttc
     6061 gtttgcgatt gcggcagcaa catcgcgggg tacctggatg tgaaagccct ggcgctctac
     6121 gccgagaccc tgcccaacgt cgtgtacgtg aaagaaaacc tgtacacctg ttccgaaagc
     6181 ggcatcaacg agatcaaggc cgccatcggg gagcagaacc tgaaccgggt ggtggtggcg
     6241 tcctgcactc ccaggaccca cgagcccctg ttccgggcca cctgcgccga ggcgggcctg
     6301 aatccctacc tcttcgagat ggtgaacatt cgggaccagt gttcctgggt gcacatgcag
     6361 gaacgggagg acgggaccgc caaggccaag gacctcatcc gcatgggtgt ggccaaggcg
     6421 gctctcctcg agccccagga gcccatccag tcggagatgg acgccaaggc catggtgctc
     6481 gggggaggaa tcagcggcat gaccgccgcc ttggccctgg ccaatcgggg ctaccaggtc
     6541 tttctcctgg aaaagactcc ccggctgggc ggtttgctcc ttcggttgag ccgcctggga
     6601 ccctccaacg cggacgcctc ggaactggcc gccaaatacg ccgaaagggt tcaaaagcac
     6661 ccgctaatcc aggtcatgac ctcgaccgtc gccgaagaag tcaagggcta catcggccat
     6721 ttccaggtca ctgccgtccg gaacggaaac cgtgaggagt tcggagtcgg ggtgatcatt
     6781 gtggccatcg gggccgaggt cctgcagccc cacggcttgt ttcaatacga cggcaagtcg
     6841 gtcatcaacc agctcgaact cgaagaacgg ctcaagtccg aagacttttc cgccggaagc
     6901 gtggtgatga tccagtgcgc cggggcccgc aacgaagatc ggaaatactg ctcgcgcatc
     6961 tgttgcatgg tggccatcaa gaacgccatt gccctcaagg aacgaaaccc cgacgtcggc
     7021 gttcgggtcc tgtaccggga catccaggcc tacgggacgg aaaacgaggc catgttccag
     7081 cgtgctaagg aactgggggt gctgttcctc aagtacgatc cggcgatgcc tcctcgagtg
     7141 gagccgggca aggtggtggt gttccacgaa accctgggcc gggacctgac cctgcctgcg
     7201 gagttggtgg tcctgtccac gccgctcatc gcaggcccgg acaacgagac cgtttcgaaa
     7261 ctcctccggg tttccctgga cgagaacaag ttcttcctcg aggggcacgt gaagctcaag
     7321 cccctggatt ttgccacgga cggcgtctac ctgtgcggca acgcccacta cccggcgacg
     7381 gtgagggaag ccgtctccca ggcgctgggg gccgcttccc gggcttccat tcccctttcc
     7441 aagaaggtca tgaccgtcga gcccatcgtg tccaccctgg tggatgaaga cgcatgccgg
     7501 ggatgcgggc tgtgcgtggc cctgtgcccg tacggggccc tcgaggtggt tcgaacggaa
     7561 aaggggcgca aggtgagggt cattcccgtg gcctgcaaag ggtgcggggt ttgcgccgct
     7621 acctgctacc ggcacgcatt gagcgtcaat tcgtacagcg atcagcagat cggggaacag
     7681 attcgcgcct ttctgggaga gtgatcgagt cttttgcgag ggctgcccgg gcagtttcct
     7741 gcgcccgggg gacgaaacct aggttaggag gttaggaaca tggcggattt cacgcccaag
     7801 gtcctggttc tttgctgcac ctggtgaggg tacgctgcgg ctgacttcgc tggagtttcc
     7861 aggatgcaat atacgacgga tgtgcgaatc atccgtttga tgtgtaccgg tcgagtggac
     7921 cccacgatga ttgccgaggc cttcatcaat ggtgccgacg gggtcatggt ggtgggctgc
     7981 cactttgggg actgccatta catcacgggc aatttccagg gaaaagtgaa ggtgggactg
     8041 gccgcccggg tcttggacta tgtgggaatg aaccctgcga gggtggagtt caaccagtgc
     8101 tcgtcggccg agggggagcg tttcgtgagg ctggtgagcg ccttcgatga gaccattcgg
     8161 gccctgggtc ccctggggag ctccgaccgg gtgcccatgc cccggctccg ggacaagctc
     8221 accatcgccc tcacggccct ggcccgggaa aagcttcgct gggtggtggg taaattcacc
     8281 gagttcgaga ccaacggaaa caagtacggg gaacgcttca ccgaacacga aatgtggcgg
     8341 accctggaca ccatcgtcat ggacgaggtt gccacctacg agatcctgca tgaattggga
     8401 cagggtccgg cttcggtcaa ggacctggcg gcccggctcg agttgccgcc gccccatgtg
     8461 ttcaaatata ttctggccct tcagaggagg gggttcgccc gcctggcggg ggtgtccgat
     8521 cactcgccgc gctacgagtc ggcggacccg tcggcgtcgg cggcttgagt cggcggcatt
     8581 ccccggaccc gaagagaccg agcggactcg atccgctccc ctatacgtgg aggatggaac
     8641 ggtgtcggaa aagcgagtga ttgtcattgg gggtgacccg gccggtgtgg ccgcggcgtt
     8701 ggcggaggcc gaggccggaa gccgcgtgac tttggtggag tcttccccca gcctgggcgg
     8761 ggggcgaatc ccgcaaaccc ggattctcga cgcaggggat gccttcgtgg atcccaacct
     8821 cgaggccctc atgcgtcatc ccaacgttcg agtgatctcc aatgcccggg tggaacgggt
     8881 ggctgccgaa aacggcggat accggttcag aatacgacgg aagacccccc gggtggactg
     8941 ggaaaagtgc aacgactgca aggcctgcat ccgggtttgt cccattcaca tgtacgatga
     9001 cttcaacgaa ggggtggggt ttcgaacggc catcgacttt ttcaatcctg aaacgggcca
     9061 gtataacatc ttcaaggaag atatgcccgt ttgccaggcg acctgccccg ccaacttgga
     9121 catccgctcc tacgttgggc tcatcgccga cggaaaatac gaccgctccc tggccaagat
     9181 ccgggagcgg cttcccttcg ccctttccat cggccgggtc tgcccgcacc cgtgcgaaac
     9241 cgcctgcaat cgcggctaca tggacgaacc catctccatc tgcaccttga agcggttcgt
     9301 ggccgactac gagatgaaga gcggggttcc ggctcccgtc gaggtccccg accggtttca
     9361 cccggagaag gtggccatca tcggggccgg gcctgcggga ttgacctgtg cctacttcct
     9421 ggccaaggcc ggataccact ccgtggtatt cgaagcccta cccgaacccg gggggatgtt
     9481 ccggtacggg attcccgaat accgccttcc cacctcgacc ctcatgtacg aaatcaattg
     9541 gattctctcc cacggggccg aactccggtg caatacccgc atcggcaagg acatcgactt
     9601 cgaagagatc cggaagcagt atgacgccgt ctttgtgggg gtgggagccc acgcgggcat
     9661 gaagcttcag atccccgggg aagacctcga gggggtggtg gacggagtgg atttcctccg
     9721 ggacgtcaac gggggaaaga aggtcaacgc caagggcaag gtcatcatca tcgggggcgg
     9781 caacgtggcc atggacgcgg cccgggtgag ctggcgggaa ggctttgacg aggtccacat
     9841 cctctaccgg cgcaccaaga aggaaatgcc cgccagccct tgggaaatcg aggcggccga
     9901 gcacgaagga atcaagatcc aatacctggt ggcccccatc gaggtcatcg gggaaaacgg
     9961 acggatgaag gccttgaaat gcctgcgcat gcagctggga gaacccgacg ccagcggcag
    10021 gaggcgcccg gttcccattc ccggctcgga attcgagatc gaggccgaaa ccctgattcc
    10081 cgccatcggc cagcgcccgg acctgacctt tgtcccggaa caggggggcc ccaccatcac
    10141 ccggtggaac accttttccg tccatcgaga cacctacatg accaatgttc cgggggtttt
    10201 tgccgcgggc gatgcggaaa ccggaccgga tatcgccatt cgggcctgcg cgggaggccg
    10261 caaggccgcc gccgggatcg tccgttacct ggaatccaag aggcaaaaat aggggttggg
    10321 tcgccatgat gtacgaaaag aaaggacgct acattgtcga attcccggat attcccaagg
    10381 cacgccagca ccatgtcgag atcgacgtgg acgcgcggcg gggaaactac cgggaagtgg
    10441 agctgggctt tcccgaggac cgggcggtcc tcgaagccaa gcgctgcctt agttgccgcc
    10501 gttgcctggg ctgtgccctg tgctgggccg agtgcaagcc cgaagccatc cgctttgaaa
    10561 tggaagacga ggtcttcgat cttgatgcgg acgccgtggt ggtttcggcc ggagtggacc
    10621 gccccgtgga acgactggac cgtcggttcg ggtttgggac tttcctgaat gtcattacgg
    10681 acctgcagct cgagaggatg ctcagccgat ccggtcccag cgcaggcctg gtccttcgcc
    10741 cctacgacgg cgagatcccg cgaagcgtcg ccttcgtcca gtcttatggt tcggcggccc
    10801 ccgccatgca ccgcagggcc ctgtgtttcg gagtgaacga agccgtcctg gttcgccaga
    10861 agctcgcgga cgcggaaatc cgggtttatg ccgccgacct ttcgtctttc cgggaagaat
    10921 tcgcaaagga cctcgaaaag ctcgacggaa tcgagatcca cgagaaccac gtcgccaaga
    10981 tcgaggccac ggaagaccaa tgcctgaggg tcttttttga agaagacggg cagacccggg
    11041 aaaagacctt cgagctggtg gtgcttctta cccagccgca gctgtccggg gaactcaagg
    11101 acctcagcaa atccctgggc ctcagttacg cctacgcgga tttcctggcc ggaaacggcc
    11161 ccggggtcat ccgcaccgaa aaggaatcca ttgggctggc cgcccaggcc tgatttcgtg
    11221 ccggggatcc cttccgcaac agacgcggag ctgctgcccg cacccggtcc gtctcccatc
    11281 ctgtcctcct tccgcttgcg ggaggaggac ctttcattgg tggggactcg aatctcgctt
    11341 gccgtctcga taccgccttt cccccacggc ggggtgggcc nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn actcgaatct cgcttgccgt ctcgataccg cctttccccc
    11521 acggcggggt gggccggagg atgcccggat gagtggttcc gatttcttca aagccctggg
    11581 aatcgaggaa ggggacaggg gggcggtgaa gcggtttgcc gaggtttccc gggtgcccgt
    11641 ggaccggctc cggcattacc atgcggccaa catcctgccg tcggggcccg accgggatcg
    11701 ggtctgcgcc gcggcgggaa tttccccggc ggagctgatg ctgcggatgg gggtgttgga
    11761 cgggcggatc ctcgcggctc tccggtccaa ggcccgggaa gtcttcgagc tcctccgcga
    11821 cgacctcgaa gattcgagcg gcccgttgga acctcctccc gtcgtctaca agacggacct
    11881 cgggaaactc caccacggag actgcctggc cctcatgaaa cacctcgagg atgaaagcgt
    11941 cgacctggtt tttgccgatc ctcccttcaa tctcaaaaag ctgtatcctt cgggggtcaa
    12001 cgacgatctc aaagaggcga aatatttgga gtggtgcgaa agctgggctg ccgagtgcgt
    12061 tcgcctgctc aagccgggag gcagcctctt cttctggaac atcccccggt ggaacacgta
    12121 ccttgcggga tacctcaacg cccgtctcac cttccgccac tggatcgccg tggacatcaa
    12181 gtacagtctc cccatccgag gaaggctcta cccgtcgcat tattctctcc tgtacttctg
    12241 caaaggggaa aagccgaaga ccttccgccc ggatcgcctg cccatggagg tttgtcccgc
    12301 gtgcaaggcg gatctgcgcg actacggcgg gtacaaggac aagatgaatc cccggggagt
    12361 caacctgacg gacgtctgga cggatattgc tcccgtgcgg cattccaagt acaagatgca
    12421 cagaggttcc aacgagctgc ccgtcaagct catggatcga attctggaga tggcctcgga
    12481 agagggcgac ctcgtcttcg atcccttcgg cggttcgggg accacttacg tggtggcgga
    12541 aatcaagcgg cgtcgatgga tcggaatcga actggggccc atggacgaca tcgtccgtcg
    12601 cctggaacaa atccgcaccg acgcgaacct ggtcggaaaa atccgtcgag gctacaatcg
    12661 gttgtttacc gaagaaacgc tccatgcccg caaacaactg ggattgtgga cctgcgattc
    12721 ggtggggaag acaaagggcg aaaccccata ggacaagggc gatcggccgt gtccccggtc
    12781 ctggccggaa aggttggaaa tgaagctcta catcgattcc gcccgggttt ccgtgatcga
    12841 atctttcatg aaaaccgggg tatttcacgg ggtgaccacg aatccgcacc ttttgaaaga
    12901 agccggggtt cccccggaga ggctttcgga acttgctcga caagtgttcg acctgggggc
    12961 cggggaggtg ttttttcaaa gctgggggga ggacgcggcg gccctccatc tccggggacg
    13021 gcaactggcg gatctggacg accgggtggt ggtgaagctc ccggccaccc tcgaggggtt
    13081 cgaggcggcc tcgatgctgg cggccgcggg gaccaggacc tgcatcacgg cggtgttcgc
    13141 cgcctttcag ggaattttgg cggcgggggc gggtgcggcc tacgtggcac cctacctcgg
    13201 gcgcatgaac gatgccgggc gcgacgggca cgccgtcatc gccctcatgt ccggagcctt
    13261 gcgccgggtg gaaagcccca cggcgatcct ggcggccagt gtgcggaccg tggacgatgt
    13321 ggccctgctg gctcaaaacg gggtgcgatg catcaccctt tctcccgccg tggcccggaa
    13381 tctcttccgg gaacccctca ccctcgaagc cgtccgttct ttcgaaaccg cggcaaacgc
    13441 catagaataa aaattccaaa acaactaccg ttgtacgggc caaagccgga atccaggggg
    13501 aacggctgga aaccggcttc ggacgggatg agggttgtta ccaggacttg gttatgcggg
    13561 accgggccgg gctgctgctc aggaggcgtt tcgaaaccat ccgcccttct tgtccacctg
    13621 gatcttctcc accaaggccc cttccttctt ggtcacaatg gagccttcga agaaatcctg
    13681 ccggtcgacg acgtccccca gcttgagatt gggattgttc gtataccgca catagttttc
    13741 gagcatgagg atcgcctgct cgcgggtcac gggctcaccc cggttgattc ccgccatggg
    13801 acaacctccc ctcatgcacc atccccttcc gaatccgcgc cccatgcccg ggcctcgtcc
    13861 catcatgggc ccttttccca tccagggaca tcgccattcg tcctgttgag cctggggagc
    13921 cggttcctgc tgctgggcgt agaggctggg agctccgctc accaggatag tcaaggccag
    13981 tgccaatgcg atccaattca ttttcttcat gatcgttctc ctttcgtcga tgcaacgcaa
    14041 tccgattcat ggactccgcg aggttgccgg ccgttccatt ggccgccgac cgtgcccggc
    14101 aacggggccg gccggtcccc ctacctcgcg aaatgatgcg ggccgtaatg ggcccgggtt
    14161 ctccagccgc ctagagggcc gccacatgcg ggaacggctt cctgacgaca ggccccggcg
    14221 cctcgtgccc agtgctttcc ttgaccgtag gcggggcagg tccattcggg agaggtgccg
    14281 ccgtctccgg gctttgcctc ctgggcgtag gtcgtcccca gccccgtgaa gaagagctgg
    14341 gccgccaatg ctgcaatggt gatggtcttt ttcatgactg actcctttgt ttgggtctcg
    14401 tttatgaatt gtcgaggcat cgttcgctca cgggtcgtgc cacgcaggag gggttaggca
    14461 acaggcgtgc cggaaagctt tcgatatctg taagtagccg atagtagaga agtttcccgg
    14521 caggccgttt ccctttcata acacagcgga tcttggcttt ctccgtttgg ccccgtggat
    14581 cctcgccttc ggcttccaaa ctcgagggaa gaccggatag gaagtatcca ggcccctgga
    14641 tccggtggat aagttttacc cggggccgaa caagcaccac ccatccgttg gcggaagaaa
    14701 gggaattgga tatgctgggg ggcgagcccc ccaagtgccc gggccggatc tcgagaccgt
    14761 ccgtttcttg cgggcggttc gtcccggagg tggtttcctt tttgcacttc cccggggacg
    14821 ggcggctgcg gtgccggtca agacgaggag acacgagtgg gttttcgcat atccaatccg
    14881 acccggtggg cgtggttttc gccctggatc ctcctgggtt ccgtgtgtat cttggcgggg
    14941 attcttctga tcttgggcgt aaagaacatc caccgtgaaa aggagttcgt ggaacgggcc
    15001 ctcctttccg aggccaacgt gcttatccgt tccatcgagg cggggagccg cacggggatg
    15061 atgggcatgg gatggggccg ccgacagcac cagatgctcc tggaggagac ggcccagcag
    15121 tcggacgtcc tgtacgtggc cttggtgaat ccccgggggc aagtggtggc ccacagcgat
    15181 ccccggcaag tgggcagtgt cttgcctttg gagtttccgc ggcagggcga aacgtcttac
    15241 cggtttttgc acaaggaacc caagtccttc gaggtggcgc gggcttacca gccctggttt
    15301 cgccaaaggg ggcgcagagg aggggaatcg tgcgaggtgg ggtcttcttc gggaaaggat
    15361 atggatctct atatcctggt gggcctcgat cccggtcccc tggaggaggc tcagcgtcag
    15421 gacctgcaac acaccctggt tctttttagc gtcatgttcc tggtgggggc ggcgggcttt
    15481 gtttccctcg tctgggccca ccattaccgc accgcccggt gctccctgaa agacatccag
    15541 gccttcacgt cgaccctcgt gaaccagatg cccgtgggcc tggtggccac ggacctcgag
    15601 ggccgaatcg agcgggccaa cgaggcggcc cggaaccttc tccggccagg gggcgggtcc
    15661 gtcgagggaa acatccggga atatccaagc tttctccccg tggttcaacg gctcgggaac
    15721 gagcaggaaa tggtcgaaca ggagatcctg tgcggaagct cgggaaggga gcccgtgccc
    15781 ctgctggtga atgccgctca catccgcgac ggtgaaggcc gcaaagcagg gtttgtcttc
    15841 ctcctggccg acatgagaaa cgtcaagcag ttggaggagc agcttcgaag gaacgaaaga
    15901 ctggcgggcc tggggcgact ggcggccggg gtggcccatg aaatccgcaa tcctttgagc
    15961 tcgatcaagg gttttgcgac catattggcg ggaaggagcg gggaggacga gcggagccac
    16021 aagattgccc acgccatggt ccaggaggtc gagcggctga accgggtggt gaccgagctt
    16081 ctcgatttcg cccggcccac ggagcttcac aagcggccca cggcctgcag agagcttatc
    16141 gagaactcgc tgcggttggt ggagggagac gccgggaagc agggggtgag agtcgagtcc
    16201 cgagtggaac cgggggacct cgaggtggag ctggacccgg accgtttctc ccaggtgctg
    16261 ttgaatctct atctcaatgc cattgaagcc atggagacgg ggggaaccct gcgagtggag
    16321 gtgctgaggg aggcgcaggc ggcggttttc cggattacgg acacggggtc cggcattttt
    16381 ccggaaagcc ttccccacgt ttttgacccc tacttcacca ccaagcctcg gggagtaggc
    16441 ctgggcctgg ccaacgtgca caagttcgta gaagcccacg gaggagaagt ggaagtggaa
    16501 agcacccccg ggcgcggcac ccgctttgcc gtccggttgc ccctcttccc gggccgacca
    16561 tcgggggaga agcgggccgg ggagacggga tgatgtcggt cgggaaacgg catggaccat
    16621 tcaaggcggc aagggaggaa ccatgaccaa agaagccggg accctggtgc ttgtggtgga
    16681 cgacgatccg gcccatcgcc tcatgatcac gacccttctc gaggaatggg gataccgcac
    16741 ccgggatgcg gaagacggga agaaggccct ggatttcatc cgggcaaacc ccgtggacct
    16801 gattctcatg gatatgcgga tgcccgaaat ggacggcatc gaggccactc ggcagatcca
    16861 cgggcacaat ccggccattc ggattgtcat catgaccgcc tattcttcca ttcccacggc
    16921 ggtcgaggcc ctcaaagcgg gcgccttcga ctacgtgacc aagcccatcg acttcgaagc
    16981 cctccggctg tccatggaac gggccctcga acatacccgg ctgctgcagg aaaacgaaac
    17041 cctgcggcgg cagttggccc agttccaaat cccggaaatc ctgggcggca gtcccgccat
    17101 gcgaagactc atggacatga tcggcctggt ggcgccgtcg gaagccaccg tgctcatcac
    17161 cggggaaagc ggcaccggca aggggctggt ggcacgagcc atccatgcca acagccaacg
    17221 gcgcggcaaa tccctggtgg aggtgaactg tgcggccatt cccgagaccc ttatcgaatc
    17281 ggagctcttc ggccacgaaa aaggggcttt caccggagcc gaccgccagc ggcgggggcg
    17341 cttctccatg gcggaccagg ggaccctctt cctggacgaa gtcggggaac tgcctctttc
    17401 catgcaggcg aaactgctca gcgtcctgca gcaggggcac atccagcggg tgggaagcga
    17461 cgtctcgatt cccgtggatg tgcgggttct tgcggccacc aatcgaaacc ttccgcagat
    17521 gattgccggg ggcgctttcc gggaagacct ctattaccgc ttaaacgtcg tagccctgga
    17581 ggtcccccct ctgcgggagc gcatcgagga catcccggtg ctggtggggc atttccaggc
    17641 tcacttcgcg gagaagaaca aaaagatcgt caagggattc accccccagg ccatggatca
    17701 cctcctgaga tattcctggc ccgggaacgt ccgggagttg gagaacgcgg tggagcgggc
    17761 ggtgatcctc ctgcgggggg aatacattac ggaaagagag ctgcccttgt ctctccggca
    17821 acccgctccc ggggaaggca cccggaagga ggagcgggaa ggggcggacc tgccggaggg
    17881 cgaatccacc ctggcggagt tggaaaggca gcaggtcctt cgagtgctgg aagaaagcgg
    17941 aggcaacaag agcgaagccg cccgccgcct gggaattacc cgccgcaccc taaaactcaa
    18001 gctcaagaag tacgaggaag aaaagggcac gacctgacct ttttctccaa tccgcccggt
    18061 tccgtccctg taaaggaccc tccttttccg gcccctgcaa ctcccgtttt tcgtttcgcc
    18121 cccccgcccg gggggcacgg gaacccttcc tttcgcagtg ccgaatcacg ttgacgctca
    18181 ggggcggggt tgctatcaac acatgagtcc aaggggatcc ttggggaagg ggatccttgg
    18241 ggacggggct gtcctcgtcc tttccgttcc ttccagcctc gatgggtgga aaaccttctc
    18301 ccttgccttg tgagaaggac ccgaaaccag gccgtggttt caacgtcgag ctcgatcgag
    18361 gcgggattcg gagctcctgg cgtctgccgg gtttcggttc tctccagcgt gatgccggga
    18421 tcgaacacgg caccgaagac gggaacggat tgaaactccc catcttacga taagcttcag
    18481 tcgcagatgg attcatgggg gcaaaaggct cgattcgatg attgaacggg atttcgacac
    18541 agccttcatt gccgacctgg cattgaggga gaagcaaata cagcagaact atcgacccat
    18601 catcgccgtt cacaagtggt ttgcccgcag gccgggaacg ctcttccggg gtttgctgct
    18661 ctccgagttc gtgaatgctc ccctgagaga ttccttctac cgcgcccacg accttagtgg
    18721 gattcgcatc ggggacccat tcaatggagg cggtaccccg ctgatcgagg ccaatcgtgt
    18781 gggttgcgat gttgtgggcc gggacctcaa ccccatgtct taccggatcg tgaagcggga
    18841 aatcgagcac cttgacctgg atgcctaccg ggctgtcggt gatgcattgg taagaaccct
    18901 ggatgaacag atcggacggt tctatcggac ccggtgttcc cattgcggct cccggagatg
    18961 cccatacttc tgcgtggggc tacgggtatc atcaagaaat gttcaattcc cgccgtccgg
    19021 ccggccttga agtgagttgc cgtttcccgg ccgctcagtc tgacgaacga atacgggatg
    19081 ccttgcgaac ctgtccgatc tcctcagtgt acggatttcc catagggctg atccaaaagg
    19141 tgatgtccgc ccacctccct gatagccctg cggttcatgc cggggtccac ttcctcatcg
    19201 gaccgggcct acatggcggc tcaccatgcc ggggttgaaa tcacacaagg acatgggcgg
    19261 acctcgcatc gcaaaattcc ggcttcaccc cgagcgctcc gttcagtgaa tcttcaggat
    19321 gaaggaaaag agatggtaca ggaaaaagga aacgacccct gccaccacgg gggtaaggaa
    19381 ccaggaaagc acaatctcct tcatggctgc catatcgatg gtttggagcc ctctaacata
    19441 agccgccccc gccacgccgc cgatttgggc ctgattcatc gaggtgggaa agcccaaaac
    19501 tgccgctata tgagcggtaa cgctctgagc gaatttggcc ccgactccta cgctcaaatc
    19561 cacttgaacg aggtctttgc cgactttctg aagaagcgga acgccccagg tcagcgcccc
    19621 gatggctatg acaatgcccc caatgaatcc ggcctgcatt tccgtggtga gtttgagtcc
    19681 aaaaaagacc cccaccgcgt tcccggtatt gttggctccc aacgtatagg aggaatagca
    19741 ggaggcgatg aacagggcat atcccaggat tttctgcaca gtgtccgatc gacctttgat
    19801 cgtccaatcc gttgcccggg taaaaaccca gccaaaaacg gccgccacga gcggggtcgc
    19861 gacccacgtc acacaaagga acactatggt gcttccccaa aagaccggag cccccatggc
    19921 gatggctgtg cccacaacgg agaaacaggc caattgagag gttgaaaccg gccatctcat
    19981 ccatgtgttg gccgcggtga ccactgcggc cgtgaacatc atggtcatgg cccccagcgg
    20041 cgtcatgtcc gtacctttga taatgccggt tcctatggtc tcgacgacgt gccggctttc
    20101 aaacgtggcg ccgagcaagg caaagagtgc aaccaaagca gtggccgtca acggcctttt
    20161 gacgatcccc gatccatagg ccatgcccat ggttgcccct gtgtagtgcg acccgattgt
    20221 ccagccgaag aagagaccgg aagcgaccat tgcgattgtg atgatgtcca tgaggaacct
    20281 cctgtgtgta tcagaacgat gaacggctat cacgccttga tggatttccc tcaaggcaca
    20341 tcctgcaagc ccgattccct ttcttagatg gaactctgca cacccttttt cgtcgcttac
    20401 cggtgttggg catcgggtct ttccagcatc gccgtgatcg gggtccttgc ggtgtcctgt
    20461 ccgtgagccc gtcccagcgt tgcttccggg gtaatgcgag acgacggttc gaccgacaag
    20521 agagccggag aacccctatt ccaacgatta ggcattccct gtcgactttt ccgtccctgc
    20581 tccagcgatt cgtctcaatc gaaataagag atacacgatg acatgtttga atgtcaacag
    20641 aacaatgtaa caacggttat aaaatagtcc agctcatgcc tgccttgata atgtatcaga
    20701 tatcagctag acagctcaaa gagaacggga ggcaccgttt tggagaaggc ctccgactcg
    20761 gaagttgtgt gtgtcaaggt ctgcaaattc gctcacggca tttgggtatt ttccgcacgc
    20821 aaattccgat tggcaagggc cgtcgagaat catgcaggga ttcagactgc gggggcgaaa
    20881 caaaaggggt aatgtttcag aatggattga cgagatggca gcgattgtcg gattaactgg
    20941 cctcttacga ggccgttttc attggaaaat gggcggagag tcttcgccga actcatccgc
    21001 ccgaaaccgt gagcgcaatg cgccccaaag gagggaatca tgataatcgc tgttgtgggg
    21061 gcactcatgg atgtttcact caaaggcaaa ggctatctcc atggctgaag ccctctgaag
    21121 gctcgaagtg cgcaggcatg cggctgtgag gacatggatt cgtgctcgcc tgatttgagg
    21181 aatgcaccga tctcatccac ctgcggcgat accgatgccc ggaagccgct gtgtgattcg
    21241 gctcaagccg cgacgctgct tcgatagatt tcactgccac atagagacta tccaaacgaa
    21301 gatctcccac agaacaaaga cggacccatg gtttgcaggg ttttcccgat ctcgtcgggg
    21361 gcattggctc agggcgctca tgcggaatgc gaaagcctat ctgtccgata cgttcgaggg
    21421 cgacccggtt gaagcctttg atcagctgcc gggggcgggc aaggttccgg taagccggtc
    21481 aatctaatcc ggcggggaat ccactttgtt gccaccctac cgatcagtgc cgtcgtgtcg
    21541 gagccagaag tattataggg atgcctcaac attgacccaa aaggaggcat tccatgacgg
    21601 aagagcaaaa gaagcaggta ggggcatttc gattcggagt catccaatgg ccttgtgaac
    21661 cgattggaca tggaccctgg ggaacaggag gggcttctac gggagaagtg ttttcggaaa
    21721 taaggcatcc cctatagccg aaagtgtcgc atcgcacaca cgactatcct gaatcggatg
    21781 aaatgccacg ggggttcggg gggcagggtc gagtcgctct atcccgaaga gcgctttgac
    21841 ttgggcagga gcagggcctc ggacgaagag accgttgatt gcggccaccc aatccggggg
    21901 aatcgggcca ccgattccgg ctgaatccgg ccaggcactt cggctgaatc cgtcccccgc
    21961 aactcccgtt tttcgtttcg cccccccggc ccgggggcac ggggaccctt cctttcgcag
    22021 tgccgaatcg cgttgacgct caggggcggg gctgctatca acacatgagt ccaaggggat
    22081 ccttgtggac ggggctgtcc tcgtcctttc cgttccttcc agcctcgatg ggtggaaaac
    22141 cttctccctt gccttgtgag aaggacccga aaccaggccg tggtttcaac gtcgagctcg
    22201 atcgaggcgg gattcggagc tcctggcgtc tgccgggttt cggttctctc cagcgtgatg
    22261 ccgggatcga acacggcacc gaagacggga acggattgaa actccccatc ttacgataag
    22321 cttcagtcgc agatggattc atgggggcaa aaggctcgat tcgatgattg aacgggattt
    22381 cgacacagcc ttcattgccg acctggcatt gagggagaag caaatacagc agaactatcg
    22441 acccatcatc gccgttcaca agtggtttgc ccgcaggccg ggaacgctct tccggggttt
    22501 gctgctctcc gagttcgtga atgctcccct gagagattcc ttctaccgcg cccacgacct
    22561 tagtgggatt cgcatcgggg acccattcat gggaggcggt accccgctga tcgaggccaa
    22621 tcgtgtgggt tgccatgttg tgggccggga catcaatccc atgtcttact ggatcgtgaa
    22681 gcgggaaatc gagcaccttg acctggatgc ctaccgagcg gtcggtgata cgttggtaag
    22741 aaccctggat gaacagatcg gccggttcta tcggacccgg tgctcccatt gcggctccca
    22801 ggatgcccac gtgaagtact tcctctgggt caaggtcatg aactgcagcc aatgccggga
    22861 ggaaatctcc ctatttcccg gctacctggt ttcgggagac aagcggcatc ccatgaatgt
    22921 tctagtgtgc ggggaatgcg ggcagctgac cgaggtggag gacggaaaaa acccgggggt
    22981 gtgcaagcag tgcggccgga gtctctcacg cacgggacct gcaaaacgca accattgtcc
    23041 gtgtccccgt tgcggaagga tccatgggta tccggatgcc ggggcggggc cgccccgaca
    23101 taggcttttc gccatggaat accattgtcc tcgttgcagg acacgacaaa agggccgctt
    23161 tttcaaaatt ccggacccgg aagacctggc tcgactggat gaagtcgaat cgatggggaa
    23221 acgattgcag ccgactttcg tgcccgacga tcccattccg ggcggagatg agaccaaccg
    23281 gctccatcgc tggggctacc ggtattatcg agaaatgttc aactcccgcc agctggttgg
    23341 tctcgagttg agttgccgtt tcatcgccgc acaacccgac gaacgaatac ggaatgccct
    23401 ggcgacgaac ctctccgatc ttctcaggta tcagaacatg ctctgtcgct atgacagcat
    23461 ggccctgaag tcgctggata tcttctccgt gcacggattt cccgtggggc tgatccaatg
    23521 cgagtcaaac ctcctgggca tcccgggacg agcgaacgga gaaaatgtgg gcagcggcgg
    23581 ctggtccaac atcatcacca agttcgtcaa agcgaagtcc tactgcttgc aaccgtttga
    23641 aatccagcac cgatcggggc gaaaggtcgt catagaaata tcgggagagt ggattggcga
    23701 aaccagccgc cttttcccgg ccgtgaaatc caggggagtg gaccttgcct gctcggacgc
    23761 ggccgtggcg gacctggagc cgggaagcct ggatgccgtg ctgacggacc ctccctattt
    23821 cgggaatgtg cagtacgccg aactgacgga tttctgctat gtttggttgc gcaaactggc
    23881 ggggagaggc gtgcctgagt tctcgacagg atcgacccgg aaccctgcgg agctcacagg
    23941 caacgagaac atgggcaggg gcttggaaca tttcacccgg ggtttatcgt ccgtcttccg
    24001 gaaaatggcg attgccctca aaccgggtgc tcccatggct ttcacctacc accacaaccg
    24061 gctcgaggcc tattatcccg ttgccgtcgc cgtcctcgac tcgggcctta cctgctccgc
    24121 ctctcttccg tgccctgcgg agatgggagc ctccattcac attagcggga ccaagtcctc
    24181 cgttgtggat accgtgtttg tttgccgttc gacggggaag atccctgcaa agtggatcgt
    24241 cgggtccccc gaagggctgg cgggccttgt gagggaagac ctggaaaagc ttagaggagg
    24301 gaatttctct ccgaccgccg gggatctgcg gtgcatcatt tatggccacc tggtccgtct
    24361 tgccgtatgg gatcttcgaa agggttggaa tcgggacctg gaaacggctc atcgtttggg
    24421 ccgggtggag gagtggatcc gtgcgtttgg ggggcttgaa tccgtggaac ggcacctgcg
    24481 cgatgtcatc tccacaattc cccacgtcca acaagcgatc gcccaagaag cacagtcatc
    24541 ctacagagaa cctcccagtg aagtttcctt ttgaagcctc gttgcaggaa atccaagaaa
    24601 accccgatcc gttcgtatcg gccgtgttct cttgcctgga atccgaattt ctccttatgc
    24661 cgaaagggag cgggtttatc gaatatcccg ttttcgaatg cggttacgaa gcactcaaga
    24721 aggccacctc ggggttccga gagtttgcgg gcgaaccggt ttattcgacg gtgatatccg
    24781 tgcccatctc cctgatagtc ctgcggtcga tgttgggatt cacgccgtcg gagtgggcct
    24841 acattgccac tcagcgtacc ggggttgaag tcacacaagg acatgcgcgg actttggatc
    24901 gcaaaatacg aatgatgccc tccgccccac tcaaggtgca gggagaggct gcagcgaggc
    24961 tcaaagctct cgtggattcg gcctgccaga tgctcgtgga gggggccccc gatgtggagc
    25021 ccgacaaact tcatcgactg gacaaggcgg acacaaggaa cggtatccag gcggttcaga
    25081 acatggccgc catgggagtg ccctatgcca tgctgctcta cgagcgactc ctcggaagac
    25141 ccttctccag tcatcgggat tccgtgagcg agcttgtggg ggatcgtctc gagtctgcaa
    25201 tcgaggacct gctcgcacgc gcagggatca gctaccgcaa gacgaaacgt gccaagcgga
    25261 tcgaaggatt cgaccaggcc ccggacttca tgattcccag cgagttccat ccccaggtgg
    25321 tcatcgaggc gaaatttacc gaagatgatg ggaccgccag ggacaaggtg acgcgcattc
    25381 agcatctcgc ggagctgagt tctatgggcg tgccttccgg acaagcacga ttcgaagtca
    25441 ttgcctgcat cggggggagg ggtttcgggg tgcgccgaga agacaggaag aagatgctct
    25501 tggcaaccgg cggaaaagtc ttcaccctga aggtgctgga ccgcctggtg gagcattcac
    25561 gtctgaagga tttccggacc ggataagaac ccaagatgct ccggttctgc acgattgtac
    25621 ccaatcaatc cctcgagata tgattcccgt cccttttcac ttcatcttct ttctctcgtt
    25681 tcaacatcca tttcggggac gggggaggag cctatgggga gagcgtgcgg ctcattcttc
    25741 gagggatgca ccggcacccg agcttcccca tgagcagttc ccgtgcccga ggcacggcgt
    25801 caaggctttg gatgtgaggg gcgagtgtgg tataagacgg ggcagccggg cgtagacaca
    25861 atacgaggaa atcaccattc gggtggaagg gatggcccat gaaagttgcg tatttcagca
    25921 tggagatcgg actcaacgag aacattccca cctacagcgg ggggctggga atcctggcgg
    25981 gggaccacat caagtcggcg gcggacctga atgtacccct ggtggcggtc accctcctct
    26041 acaagcgcgg ctacttcgtg cagagcatca atcctctggg gcagcaggag gagacgtatc
    26101 cctattttga tccgcgggcc ttcatggagc aattgccctt caaggtcacc ctcaagctgg
    26161 agggacggga cctgcacatc agtgtctgga aatacaccca ggtcggcctg acgggaaagg
    26221 ttcccgtgta tttcctcgat acggacctgc ccatcaacgc gcccgaagac cggttgatca
    26281 cccagtacct ttacggaggc gatcagcaca ctcgaatctg ccaggaaaag gtcctgggga
    26341 tcgggggcta cctggcctta aagcgcctcg agcccggggt gaccacctac cacatgaatg
    26401 aaggccacgc agcctttctg accctggccc tcttgaaaga catggacggc aacgaggaga
    26461 agacccggga caagtgcatt ttcaccaccc atactcccgt cccggcgggc cacgacaagt
    26521 tccattacga catggcggag gaggtgctgg gagactacct gccgtcgaac atccggtctc
    26581 tggccggaga agaccttctc aacaccaccc tgctggccct caacctctcc cgggccgcca
    26641 acggggtcag cgagctccac ggggaaatct cgcgccggat gttccccggc ttcgatatcg
    26701 ggcacatcac caacggagtg caccatctca gctggacggg gcgggaattc aaggaactct
    26761 tcgaccggca cgtcccgacc tggcgccagc agccccaggc cctcaagggt gccctgaaca
    26821 ttcccaccga cgagatccga gaagccaagc tgagcgccaa gaaacggctc atcagctacg
    26881 tcaattccgc cactggagcc gggtttagcc atgaattgct gaccatctgc ttcgcccgta
    26941 gggcaaccgc ctacaagcgg accaccctca ccttcaagga catggaatac ctcaccaatc
    27001 tctcttttga ccgggtccag ttcatctttg cgggcaaggc ccacccccag gacggcgcgg
    27061 gcaaggagct catcaaggaa atcgtcaaca cggggaggca atacgagaag caactgcgac
    27121 tggttttcat ccccaattac accatctggc ttgcctcgct catgacccgg ggtacggacg
    27181 tctggctcaa caccccccgg cgtccccggg aagccagcgg caccagcggg atgaaggtct
    27241 gtttcaacgg gggagtcaac atgagcgtcc tggatgggtg gtggagggag gtctgcaggt
    27301 cccggatcaa cggatgggcc atcggggacg acgaggacca gaccgacgaa gaggcggcca
    27361 acgatctcta ccaggacctc gacgacttgg tgaccaccta ttacgccaat cccaagcagt
    27421 ggctcgccat catgaagaac tccattgccg acgtgacgcc ggtgttcaat acccaccgca
    27481 tggtgacgga atacctgcgc acgtactatc tatgacgggc ctgggccggg gcgtcctcgg
    27541 cccaggtcgc gccggaactc cccggggccg ggggaggggg acggcggcgg ctctcagctt
    27601 cggtaggagg cgttgatctt cacgtaatcg taggacagat cgcaggtata ggcgacaaag
    27661 tcgaagcctc ccatgcccag gtccaggtgc acggggatct ccttttgccg gaacaccccg
    27721 gcggcctttt cttccacgtc gacgccttcg atcggctttc ctcccttgaa cacgcaaacg
    27781 tccccaaaat gcagggtcac ctgctgcgga tccaggggca cgtcggcccg ccccgcggcg
    27841 gccacgattc tgccccagtt ggcgtcctcc ccgaagaagg ccgttttgac caagggggag
    27901 tgagccacgg tgaaggcgac ccttcgggcg ctttccgcgt ccggggcccc cgtcacgtgg
    27961 agctcgatga acttggtggc cccttctccg tccaggacga tccgccgggc aaggtccagc
    28021 aggaccgcct ccagggcttc cccaaaaagt cggctgtcgg ggctgtccag ggcggaaaga
    28081 acggggttcc cggcggcacc gcccgccaga accagcaggg tgtcgttggt gctggtgtcc
    28141 ccgtctacgg tgatgcggtt gaaggaccgg tcggcacccg cccggaccca gtgttccagg
    28201 acgccttttc ccacggcggc gtcggtgcac acgaaagcga gaagggtcgc catgtcgggg
    28261 gcgatcatcc cggacccctt ggctacgccc ccgaccgtga cctcccggcc ccccagcagg
    28321 atccgggtgg aggccatctt cggctgggtg tccgtggtca tgatggcccg ggcaacctct
    28381 tcccaggcgt ccggccgcag ggtgttgcga agagtcgaga tggactgccg gacgggtccc
    28441 agtcgaacct gttggccgat gattcccgtg gaagccacca aaacggtctt ggggtcgact
    28501 cccagggatt ccccggcaat tcgagccatc tcccgggcct tctcgaggcc ctcgtccccc
    28561 gtgcatgcgt tggcgatccc ggcattgacg aggatcgaac agatgcggtc cgacttgagg
    28621 tgctcccggc atagctgcac gggagccgcc gtgaaacggt tccgagtaaa aacccccgcg
    28681 gcgacagccc cctcgacgga ctcggtacag atccaggcca aatccggccg accttggtaa
    28741 cgcatgcccg aggcctcggc cgcgacccga aagccgggaa cctcgaaagg ccctctactt
    28801 accacaacat ttcttgtatt ttctaccgct tccgcagggg catgggtcgt ttcggccaac
    28861 tttcttgtcc tttctagcgg ctgccggcct ggacgatcct ccgtcgccgg gaccgtaaaa
    28921 catgggttgt tcttcctgct ccctctttag atcctggacc tggtcctcat gctggatctg
    28981 aatgtgatag agcaggcgga ccgtttcttc cttgatccgt tggatcattt cctggaagag
    29041 ctgatggcct tcccgtttgt aggcgatgag cgggtcctgc tgcccgtacc cgcgaaggcc
    29101 gatcccctcc ttgaggtggt ccatgttgag gagatggtcc ttccagtagg tatcgacggt
    29161 ctgcaggagc agatagtttt ccaggtccct catcaccgcc tcgccgaact ctttctcccg
    29221 ggcttcgtat tgctgaacga cctgttcaag gaggctttcc ctcaattgtt cctgggagac
    29281 gtcttcgagg gattccaccg tgaaggtcgc ctgcatccca aagaggcgcg agagctcttt
    29341 gttgagggtc tcgaggtccc agtcttcggc gtaagtcttt tcgctggtgc agtcggtgat
    29401 caggccttcc accaggtcct ccaccatgtc cataatggca ggtttgaggc tctctccccg
    29461 caaggcttcc cgccgctggc ggtagatgac ctcacgctgc tggttcatga cgtcgtcaaa
    29521 ctcgagaagg tgcttccgga tgctgaaatt ctgggcttcc acgcggcttt gagcgttctc
    29581 gatggcgcgg ctgatcaacc ggtgctcgat gggttcgtcc tcctccatgc cgatgcgctg
    29641 catgagaccc gagatgcggt ccgcggcaaa gatgcgcatc aggtcgtctt cgagggagag
    29701 gtaaaaccgc gaggaccccg ggtctccctg gcggcccgcc cggccccgaa gctggttgtc
    29761 gatccgccgg gcctcgtgcc gctcggtgcc ggtaatgtgc aggcctccca gcttggcaac
    29821 gccctccccc aacacgatgt ccgttccgcg gcccgccatg ttggtggaaa tggtgacggc
    29881 cccgggttgg ccggccaggg ccacgatctc ggcctctttc tcgtggtgct tggcgttcag
    29941 cacttggtgg ggaactcctt cccgcttgag catctcgctc agtttctcgg atttctcgat
    30001 attgatggtt cccaccagca ccgggcggcc catccggtgg agttccttga tttcccgggt
    30061 caccgcccgg aacttttcct tttccgttcg gtagatgcaa tcggggtggt cggtgcggat
    30121 catcttcctg tgggtgggga tcaccaccac gtccagcttg tagatcttgg caaattccgc
    30181 cgcttccgtt tccgctgtac ccgtcatgcc cgccagtttt tcgtacatgc ggaagtagtt
    30241 ctgaaaagtg atggaggcca gggtttggtt ttcgttttcg atccggacgt tttccttggc
    30301 ttccagggcc tggtgcaggc cttcgctgta ccttctcccc ggcatcaggc gccccgtgaa
    30361 ctcgtccacg atgatgactt ttccgccctt gacgatgtag tccacatccc ttttgaaaag
    30421 agcgtgagcc cgcagggcct ggtgcacatg gtggagaatg gtgatatgcc ggggatcgta
    30481 gagattttcg actcccagga ggttctccac ccgggcgacc ccttcctcgt tcagggccac
    30541 cgttcggctt ttttcctcga tggtgtagtg ggcttccttg tggagctggg ggatgatccg
    30601 gttgatgcgg tagtagagtt ccgtggattt ctccgcgggt cccgagatga tgaggggggt
    30661 tcgggcttcg tcgatgagga tggagtccac ttcgtccacg atggcgtaat ggagatcctt
    30721 ctggaccagt tcttccaacc ggaacttcat gttgtcgcgc aggtaatcga agccgaattc
    30781 gttgttggtg ccgtaggtga cgtcggcccc ataggcttcc tggcgttccc ggtcgtccag
    30841 tccgtggacg atgcagccga cgctgaggcc cagaaagcgg tagatctttc ccatccattc
    30901 gctgtcgcgc cgtgccaggt agtcgttcac cgtaatgagg tggactcctc tgcccgtgag
    30961 ggcgttgagg taaatgggca gggcggccac cagggttttt ccttcgccgg ttttcatctc
    31021 ggcaatcatg ccctggtgca ggacgatgcc gccgatgagc tgcacgtcaa aggggcgcat
    31081 gcccagggtg cgaaccgaag cttccctggc tacggcaaag gcttcgggca gcaggtcgtc
    31141 caagggttcc ccgttgccca agcgttgccg gaattccggg gtcttggccc ggagttgggc
    31201 gtccgacagt ttacgaacct gtggttcgaa acggttgacc tcgtccacca ggggtgcgat
    31261 tttcttgaga gttcgctcgt tgtggcttcc aaatattttc ttgagtaatg cgccgatcat
    31321 aggacctcga acactccttc ctcatgaggc acgacatttt caagaacaaa ggcacgggac
    31381 ccgtgccttg ggatatccaa tccaaactca acaatccagt atatcccacc tcggaccaca
    31441 atgcaaaccg gttgaaaaag ccccccgcgg gtggattgac ggccgcccat ccccgccgga
    31501 tgcaaagaaa gacgaccatg cgtctcgaca gggacgaggc cgatgcgtcg aagccggaca
    31561 ggggagtgat gggttcctga attgaaccat tccctgtgat ccttccgcag tcagctcgtc
    31621 ggcattgtat ctcaggactt tgggacatcc cctgttgagg ggagccaagg ctgctcaacc
    31681 acaggaaatt ccatagccaa gggctagaag ctgatcgctc gatttagagg gttatgtcga
    31741 tgcaattcga gccggaagcg gttgagcggc actcaggtcc ggcctcccga cggattccta
    31801 tcccgccagg tccaggacca tggccacctc gtcgcactcg gggaacttgg gacaatggag
    31861 gcagtcggcc caaatcttgt ggggaaagat gtttttgtcc accaagcgaa aacccaggcg
    31921 ttcgaaaaac ccggtttcat aggtgagggt gaacacccgt cggattccca cgctgcgggc
    31981 gtcttccacg caggcattca ccagcttgcg acccagcccg agcttctggt ggctgtccag
    32041 aacggccagg gatcgaattt cggccaggtc ctcccagacg atgtggaggc tgcagcatcc
    32101 caccacctgt ccggtctcat cgtcttcggc caggaagatg tctctgaggt tggtgtacag
    32161 ctcactcaaa gaccgggcca ggagccgttc ctgctgagcg aaatgggcca ggatcttttg
    32221 gacgaccacc acgtcttggg tcttggcttt gcgaatcatg ggcctgcctc tgcggaattc
    32281 ctgccccggg gccttttagc gccggtgttg aaggatggta tggacggatc ggagaaagga
    32341 gtcgatttgc tccggggtgt tcatgtgccc cggagagacg cgaacggtgc ccgagggaaa
    32401 cgttcccagg gttcggtgcg cttggggagc gcagtgcagt cccgagcgaa cggcaatgtc
    32461 gaaggccttg tccaggagaa acgccacttc gtccgggctc atcccttcca cggtgaagga
    32521 gaccacgccg gtaccccgca ccgacggtct caggacctcg acgccgttca attccgaaag
    32581 gccgtcctca atctcggcag ccagcttcag ttcgtgattc aggacggttt cgagtccttc
    32641 ttcgagaagg taccggattc cggctcccag gccggcgatt cccggaaagt tgagggtgcc
    32701 gctctcgtag cggtccggac agaatcccgg ttggcgggga tcttccgaac gactgcccgt
    32761 tcctccttcc acgaggggaa gtacgtccag gtcctcccgg acatagagaa ccccgacacc
    32821 ggctgggccc aggagccctt tgtggccgga acaggccagc atgcccaaat cccattcccc
    32881 ggcggaaatg ggttgaaccc ccgccgtctg ggccgcgtcc aggaagaggg gcacgccgga
    32941 ttccctgcac atgcgcgcaa tccgggggat cggcagcagt gctccgttca cattggacgc
    33001 gtgggtgaga accaccaggc ggagtccgcc ctcgagggcc cgggcgaagc cttccagatc
    33061 caactctccc cgacggctgc aagggatgat ctcggtacgg acgcccaggg tctcgaggcg
    33121 cgccaacggt cgcacgacgg aattgtgttc gaggccggag gtgaggacct tatctccggg
    33181 cctcaaccac cctttgagga ccacattgat gctttcggtg gcgctcttgg tgaacgcgac
    33241 ccggtccggg tccgggatcc ccaggatttc ggccgttctt ctgcgggtgt ccgaaaccat
    33301 ggccaaagct tcccgggccc gccgatggga cgcccggccg ggattggctc ccacctccgc
    33361 catggcatgc tgcaccgcgg cgtacaccgc tggtggtttg ggaaacgtgg ttgctgcgtt
    33421 gtccagatag atgctcatga attccgtccc aggccgggtt cccttccgct tgcccccgtc
    33481 tccatctcct tcccaccgaa agcgtttccc ctattcgctt cacctccacc ccaaccaacc
    33541 aactaaccca acaaccccaa ctcccccccc acgaccctct cttccccgct gtagacggtg
    33601 aatttgcccc ctcgagcgta cccgatgagg gtgatattca gggacccggc cagttccacg
    33661 gcaagggtgg tggccgtgtt gcggctcacc agcatgggca gtcccacctt ggccgccttg
    33721 atgagaatct ccgaggtcag gcggccggtg gtgagaatga acttgtcttc gagggaaatc
    33781 ctcttttgga acacgtggcc cacaatcatg tctacggcat tgtggcggcc gatatcgttc
    33841 cgaaacagga ggatttcctc cggggatgcc agggcggtgt tgtggacccc atgagttcgc
    33901 cggtaggttt cactcaaacg gttgagatcg tcgatgcgat cgaggacctg ccgggggctc
    33961 actctcaggg ggctttggat cttttgggcg agcagggaat ccagggcgta atagaagacg
    34021 gagccttttc cgcatccgga cgtgatggtt ctcttttccc acaggttgcg aagaaggggc
    34081 gaggctccgg gtacaaaagc ctccaccttc cccagttccc catcgatttc gattcgctcc
    34141 aggtcttggg gttccttgag aaaaccctcc gcatgaagaa aaccgatcgc cagttcgtcc
    34201 agatggtggc cggcgcaaag aagtgtgacc atttcctccc cgttgaggta gagggtcacc
    34261 ggctgctctt tcacgatcca ttgcctgcgc agaacccctt cctggttctt gtcgaaaacc
    34321 caggtggaaa cgggggctgt ttcttcgtcc tcatcccttg agacgtcttt gtccggaagt
    34381 tcttggtcgc ggagaaactc tttggggacc atggttcact cctgggggat gtccgagtcc
    34441 gcctccggga gaggaggacc ctcgaggcgg cctcgagaaa tccagcgaaa ataatcgtcc
    34501 acgattcggg catgatcgaa ggcaaggggg tgaggcaggt tgtccgccga aaagattccc
    34561 agttgagaag cgtcgtctgc ggccgcgggg gttcctcggc ccacggccgt gaaaacgacg
    34621 ctcaccgtat ggtgacgggg atcacgggcg gggtccgaat aggcgcgaaa ctgccgaagg
    34681 tccaggacat ccagcccggt ttcttctttg gcttccctgc gtgccgcttc ctccagggtt
    34741 tcgccgtagt ccacaaaccc gccgggcagg gcccatccgg gaggagggtt ctttctgcgg
    34801 atgagaacaa ggtcgttggc ggtcgccgtc cggatgagga tgtcgacggt gaggagaggg
    34861 ttcctgtaag tggtcacctg ggttccgcag cgggggcatt ggattccgtg gaggacgggc
    34921 atgatgcttt cactcctctc tcgggcgttt ctttcgcaga aggaaatgtt cgaggaacag
    34981 gaccaggaaa atccccacca cgaggccgtt tcctaggaaa acccggaaat ggatgggtat
    35041 ggattccaac agggacgaag gcagaaagct gaccagggtt cccatgagga cggggacgcc
    35101 caccacgaag taatcccgtc cgctgaggtc tccttcggcc acgattccca ttgcggctcc
    35161 cacctgggcc cccatggcca cacaaagggc cgcccccacc accggggcgg ggatcatggc
    35221 cagcactgcg gcgagcttgg ggacaaaggc cgccacgacg agcaggattc cgccgcaggc
    35281 aacggcgtag cgactggcta cccggttggc caggatcacc cccggactca tactgtagct
    35341 caccagcccc acgattccca gggtcccgca gcagataccg gccgctccgt tccagaagac
    35401 cccgcgggtg accgccgtcg ggagcctctc ggtgtccgta atggctgcga ccccatgaag
    35461 acttcccagg gtattcacca ccaccgccag gtaggatgcg gccatggcgg cgacggcggg
    35521 ccaatagaac cggggtgggg tgaggaaccg catggaagga agagacaccc agggagccgc
    35581 ccccaggggg ctcgaatcca ggggttcgag gaggaagaac aggagggtgc ctaggagcat
    35641 gcccaggaga agggagaggg tcttccagaa gccctcgatc caatgggaga aggcggccat
    35701 ggccaacaca agcaggaagc tcagggcgag aatgccggcg ctcccgttgg gctgcagggc
    35761 gtctttgccc gtgaccgagg ccatgaggtg aggcagcagg gagaaggcga tgagcatcag
    35821 gatgacgccc accacattgg gcgtggcata ggccaggaga cggtgcaatt ttccggagaa
    35881 taccagcgcc atcaggagaa tgcccccggc aatggtgcct ccctggatga tttccgcccc
    35941 gtaaggggcc agaacgatga aggtcaggag gagggcggtg gagggaccgt ccaggatggg
    36001 gtaccgatgt ccccaaaggg tctggatgac cgtaaagagg cccgagagga gcaggaggag
    36061 ctggaagaaa tgcacctcct ggtccacggg gagccggagc gcccgggacc ccaaggtggc
    36121 cacgatgatc agggcgggga agatgacgat ggcccactga agaccgtaga gaagaacgta
    36181 ccttaggggg ggaggtacat cgatgccgta gacatactgg ggtttatcca tgtcggcggg
    36241 ccgggaccca agagcgttca atgctgaatt tcgaaaagag tgctgatgga atcctcattg
    36301 tgaatgttcc ggatggctgc tgaaaacagc ttgaacgaag ggaccaccac aatcttttgg
    36361 cagtgggccg cctgaggtcg caaggggagg gtgtcggtca ccaccaggct cttgagggcg
    36421 ctcttctcga tccgttctac ggcaggaccc gagaggaccg catgggtcac gcaggcgtgg
    36481 acctcccgcg ctcctttctc gagcaaggtc cgggtggctt ccaccagggt cccagccgta
    36541 tccaccatgt cgtccaacac gaccgccgtc ttgccgtcca cttccccgat gatgttgagg
    36601 gcttccgcct cgttgacgtc cgagcggcgt ttatcgatca tggccaagcc cgcctggatg
    36661 cgccgggcat aggcccgcgc ccggggaacg ccgccggcgt ccggcgagac gatgaccagg
    36721 ttgctgttga aatgttcgcg gatgtaagga agcagaatcg gggaggcgta caggttgtcc
    36781 acggggatgt cgaaaaaacc ctggatctgt ccggcgtgga ggtccatggt gaggattcgg
    36841 tgggcgcccg cccgtgtgat caaatccgcc accaaccggg cggtgatggg aacgcggggc
    36901 ttgttcttgc gatcctgacg agcgtaactg tagtacggaa ttacggcggt gatcctgcgg
    36961 gccgaggctc gcttcatggc atcgatcatc accaggagtt ccatgaggtg atcattcacg
    37021 ggatgggccc ccgattgaac cacgaagatg tcggcacccc gcacgttctc ctggatttcg
    37081 atgcggatct ccccgtcgct gaaggt
//
LOCUS       DAOOVC010000025        35568 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_785_length_35568_cov_7.455777, whole genome shotgun sequence.
ACCESSION   DAOOVC010000025 DAOOVC010000000
VERSION     DAOOVC010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 35568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35568
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_35568_cov_7.455777"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..955)
                     /locus_tag="PLM79_05320"
     CDS             complement(158..955)
                     /locus_tag="PLM79_05320"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOV85761.1"
                     /translation="MSSHTQRLVTAILVLVPVLWVIAAGSYWTWTVMVLLAALLGLRE
                     FQHLLVSDVRSSRQWRVLYYSGGLFLPAGAALGGPTGLHCGLILGLFGALLGMLARSP
                     LDSNGIRRLAQCTLGWLYIPYLLSYVLLIGQAPPGRRWLLFTLVVVVCSDAGAYYSGR
                     TWGRHKLYEAVSPKKTVEGSAGGLLLSVLAGVGIGQLLLPERTIPQTLVLSVALALIS
                     QTGDLIESMMKRISGKKDSSRLLPGHGGILDRLDSLLFVFPTSWLFL"
     gene            complement(985..1728)
                     /gene="uppS"
                     /locus_tag="PLM79_05325"
     CDS             complement(985..1728)
                     /gene="uppS"
                     /locus_tag="PLM79_05325"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOV85762.1"
                     /translation="MDRLDLKKLPRHVAIIMDGNGRWAKLKLLNRITGHQEGAGSVRE
                     IVRCCRKLEIPYLTLYAFSKENWQRPPHEVEALWLLLKRFLKSELPELLENGIRLRHL
                     GDPQGIPPDVLEDLYSVQAQTASFDKLILSLAINYGGRQEIVQAAWTYAREMAGGAIP
                     AQPPSPADFARYLYTSGMPDPDLIIRTGGEHRVSNFLLWQAAYSEFYITNTLWPDFRE
                     REFIEALLDFQGRERRFGKTGDQVQEKAS"
     gene            complement(1729..2286)
                     /gene="frr"
                     /locus_tag="PLM79_05330"
     CDS             complement(1729..2286)
                     /gene="frr"
                     /locus_tag="PLM79_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOV85763.1"
                     /translation="MLNDVYADARDRMNKALETLEREYKRLRTGRASISLVDEIRAEY
                     YGVPTPLNQLSTITVPDPRTIMIQPWDSTVIGEVEKAIHKSELGLNPTNDGKVIRIHI
                     PPLTAERRRELVKVIKKMAEEAKVAARNIRRDVNEMIKELKKEKEISEDEQFRAQEEI
                     QKITDDFIKRIDGVYGIKEKEILEI"
     gene            complement(2276..3016)
                     /gene="pyrH"
                     /locus_tag="PLM79_05335"
     CDS             complement(2276..3016)
                     /gene="pyrH"
                     /locus_tag="PLM79_05335"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOV85764.1"
                     /translation="MSHASKPRYTRILLKLSGEALMGSGSYGIDPQVLTVIAEEVQEV
                     RELGVEVALVVGGGNIFRGMSGAAQGMDRSTADYIGMLATVMNALALQQALEKHGMPT
                     RVLTAIDMKEVAEPYIRRRAIRHLEKGRVVIFAAGTGLPFFTTDTTAALRAVEIGAQV
                     LMKATKVDGVYNDDPEKDPMAVKYNQVSYMEVLRKNLRVMDATAISLAKDQNMRIVVF
                     SLKQRGNIKKVILGEPIGTVVEGVGHVE"
     gene            complement(3140..3736)
                     /gene="tsf"
                     /locus_tag="PLM79_05340"
     CDS             complement(3140..3736)
                     /gene="tsf"
                     /locus_tag="PLM79_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041440215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOV85765.1"
                     /translation="MEITASMVRELREKTNAGMMDCKKALQDTGGDMEKAVDLLRRKG
                     LAKALKRSGKEAQEGMVHAYIHGGGRIGVLVEVNCETDFAAKSEDFVEFVRNIALQIA
                     ATNPLGVRKEDVPEEIIERERGIYLAQAGETGKPAHILEKMVDGKMRKFYEESVLMEQ
                     AYVKEPEKTIQDYLNELVAKIGEKIFVRRFVRYQLGEE"
     gene            complement(3775..4605)
                     /gene="rpsB"
                     /locus_tag="PLM79_05345"
     CDS             complement(3775..4605)
                     /gene="rpsB"
                     /locus_tag="PLM79_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOV85766.1"
                     /translation="MNVVTMKQLLEAGVHFGHQTRRWNPKMKPYIFGARNGIYIIDLQ
                     RTVKLFNTAFRFVADTAEAGESVLFVGTKQQARDTILEEARRAGMFWVNQRWLGGMLT
                     NFKTIKLRIDRLKELDAMIEDGSINRFPKKEILQLQKERMKLEKNLGGIKDMTRLPGA
                     LFVVDTHREDIAVAEANRLGIPVVAIVDTNCDPDLINYPIPGNDDAIRAVRLITSRIA
                     DACIEGKERHLEKQQALGDKEEPAFEEVPAGQILKEEASGPEVEIVNPVALSRQEEQE
                     "
     gene            5151..6170
                     /gene="aroF"
                     /locus_tag="PLM79_05350"
     CDS             5151..6170
                     /gene="aroF"
                     /locus_tag="PLM79_05350"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOV85767.1"
                     /translation="MLVVMEQEATPEEIERAVQAVESRGFAARPVPGGHRVAICVLHN
                     HGSVEPSLFLGLPGVKEVIPVTRPYKLVSRESHPTDTVVRVGDACIGNGTLTLIAGPC
                     AVESETQALTIAGLVKKAGAHMFRGGAFKPRTSPYAFQGLGEDGLKILARVRRETGLP
                     VVTEAIDHEVFDLVEAYADVIQIGARNMQNFSLLRRAGRSSKPVLLKRGLSATIEEWL
                     MAAEYIMEGGNTRVILCERGVRTFAHHSRNTLDLSAVPVVQKETHLPVLVDPSHAAGR
                     RSQVIPLSRAAVAVGAHGLLVEVHHQPEAALSDGAQSLYPEQFVELCGQIRPIFKIFR
                     PDGIH"
     gene            6213..7685
                     /locus_tag="PLM79_05355"
     CDS             6213..7685
                     /locus_tag="PLM79_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOV85768.1"
                     /translation="MRLRQFPDKNEFLRMAGSSNLIPVCLEVLADTETPLSALAKVYR
                     GKGPVFLLESVEGGERWGRYSFLGSSARSVVRVYPDRVVIESGASTEEVPHHGRPLDV
                     LRAFMARFKPASPAGLPRFWGGLVGYIAYEAVSFFEAIPHDMPVDAPLAHFAIPGELL
                     IFDNLRHTLTLVAYVFLEDTSPEAAYHRAIEKLDGMRAVLAGPMPHRPTGCRDFCELK
                     PHVDPDSFRGQVARIKEYIEAGDVIQTVLSQPFSCQYPPDPWWLYRAQRYVNPSPYLY
                     FLHLDDIVLVGSSPEVMVRLENGVATLRPIAGTRPRGKTEQEDRKLADELLQDPKERA
                     EHLMLVDLGRNDLGRVAEVGTVQVTDLLVVERYSHVMHLVSNICCDLKTGCDAWDLLQ
                     ATFPAGTLSGAPKVRAMEIIAELEKEPRGPYGGAIGYVSFTGNMDLAITIRTACIQKD
                     RLVVRAGAGIVADSDPEKERLETISKAMAIQRALQLIYRG"
     gene            7745..8317
                     /locus_tag="PLM79_05360"
     CDS             7745..8317
                     /locus_tag="PLM79_05360"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOV85769.1"
                     /translation="MLVMIDNYDSFTYNLVQYLRVLGAEVRVVRNNVVSVEEIREWKP
                     RGIVISPGPCRPENAGISVSLIRETTGKLPILGVCLGHQAIAVAFGGTVVRARQLMHG
                     KTSAIQSDGKTIYRGIRSPFQAMRYHSLAVRREDLPSCLEVTAESEDGEIMGIRHREH
                     PTEGIQFHPESIMTPVGKRLLRNFLQRVEG"
     gene            8345..9364
                     /gene="trpD"
                     /locus_tag="PLM79_05365"
     CDS             8345..9364
                     /gene="trpD"
                     /locus_tag="PLM79_05365"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOV85770.1"
                     /translation="MLHESLKKVVQRQDLDETEMAEVMNDIMTGNATNAQVGALMAAL
                     ATKGETYSELAGAARTMRRKATRIQTPADTVVDTCGTGGDRKGTFNISTTAAFVVAGC
                     GVTVAKHGNRSVSSRCGSADLLEALGVRLDLEPELVEEAVQSLGIGFLFAPLFHGAMR
                     YAAQARKEVGIRSIFNMLGPLTNPAGANCQLLGVYAPQLTEMFAQALLSLGTRSAYVV
                     HGLDGLDEISVCAPTRISELKDGLIRTYEVSPEQLLGRRAEPEELLGGDARENSRITR
                     EVLGGAKGAKRDVVVLNAAAALMAAGKAPDFSRGIRLAEDSIDSGAAMGKLEALVRFT
                     SENAQ"
     gene            9387..10229
                     /gene="trpC"
                     /locus_tag="PLM79_05370"
     CDS             9387..10229
                     /gene="trpC"
                     /locus_tag="PLM79_05370"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOV85771.1"
                     /translation="MKPDILHKILERKRQEVETARRALPEAVVRRLAEERPERRPFFQ
                     RLQSPLPHGVNIIAEIKRGSPSRGILRGDLDPARQAKLYQKGGAAAVSVLTDASFFHG
                     SSRDLKEARSAVNLPILRKDFILSTYQVYESAAMGADAVLLIVRALSREFLRDCLSLC
                     GELLLDALVEVHSQEDLETATWAGASLIGINNRDLGTFRTDVEVSVRMAARLDPRQVG
                     VAESGIENRGQVERVLGAGIWNFLVGESLVRAENPVELLGRLLGRPNGKGFPGRAQKE
                     GFLK"
     gene            10226..10930
                     /locus_tag="PLM79_05375"
     CDS             10226..10930
                     /locus_tag="PLM79_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOV85772.1"
                     /translation="MNSQQMSGTVRKRGAPPFPQVKVCGLKSPGEAAECARLGADAVG
                     LVFYPPSPRFVTDEQAREICGDLPGSVTRVGVFVQEGYDSILRRIESCGINAVQLHGG
                     EDPELVRHLTREGILVIKTLFANGTPGIEDSASYAASAFLIECRGGPLPGGNARTWDW
                     SRAAPLAGRYPLVLAGGLAPENVSDAMSRALPDALDVSSGVEISPGRKDLQKVREFLE
                     AVRSTPSSRTLRRIFS"
     gene            10927..12147
                     /gene="trpB"
                     /locus_tag="PLM79_05380"
     CDS             10927..12147
                     /gene="trpB"
                     /locus_tag="PLM79_05380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOV85773.1"
                     /translation="MKPNPQSRVPTFPDPHGHFGPYGGRYVAETLMPAILELEEAYER
                     IAGRRDFHEELGKLLKDYAGRPTPLFFAGRLTAKLGGVRIYLKREDLAHTGAHKINNT
                     LGQALLARWMGKRKVIAETGAGQHGVATATAAALFGMECRVFMGTEDIERQAANVQRM
                     RLLGAEVVPVRSGTGTLKDAMNEAMRYWVGAVRDTFYVIGSVAGPHPYPRMVRDFQRV
                     IGDETREQILAAEGRLPDVLVACVGGGSNAMGLFYPFLEDAVELVGVEAAGRGIPTGK
                     HAATLGAGSVGVLHGSKSYVLQDKDGQILEAHSISAGLDYPGVGPEHAHLKDMGRARY
                     ESVTDFEALEAFRELCLCEGIIPALESSHAVAWALREAPRRPPSEVIVVNLSGRGDKD
                     LGIVAGRLDPHQPQ"
     gene            12170..12970
                     /gene="trpA"
                     /locus_tag="PLM79_05385"
     CDS             12170..12970
                     /gene="trpA"
                     /locus_tag="PLM79_05385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOV85774.1"
                     /translation="MNRIRKTFQDLEGRREKALVGFVTAGDPDIARSRELILSMCRAG
                     LDILELGVPFSDPTADGPVIQRSSARALRAGVNLAAVLELTRDLRREVSVPIILFSYY
                     NPILAYGADRLYGDALAAGADGLLVVDLPPEESQEMTSRWPGRDLCLIRLCAPTTPPR
                     RMESIARGASGFLYLVSMTGVTGSGGLDLEEISRVATLLRTCADGTPICVGFGISTPE
                     QVRAVASVADGVVVGSAFERFMESGLEDPDLGEKLGAMVRGLKAATRF"
     gene            13163..13477
                     /locus_tag="PLM79_05390"
     CDS             13163..13477
                     /locus_tag="PLM79_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV85775.1"
                     /translation="MFFFIGGVQPKTVTLEDTARLCPACGLAQARLKRIDHYVSLFFI
                     PLFPVKRGDPVVICDRCGAVSAPHPGVGEVPVVLKGGNCLRCGYPLDPSFRYCPQCGV
                     RV"
     gene            13523..14251
                     /locus_tag="PLM79_05395"
     CDS             13523..14251
                     /locus_tag="PLM79_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85776.1"
                     /translation="MPNENKILFFPMVARPCILEVLTEFLDEQAKRLKPRTYRRYEEV
                     VDLFRHCMNNYGVDELTNSADIVFYERLYYQKNLEFTAIFGPDKILPCLPYFLNFFML
                     RKVMASEGLLEAAGTVSRRLVRWLLENGYVKAEEAEEAAKVVMEAARELPAAERVARL
                     LFHYAQSHTPRYWTLELDDYFTIDKVNPGTLVLAGSNTSALVELKVPKEITDQCKEGW
                     QISLLLGKTQGGWSILETGNVYPS"
     gene            complement(14356..14949)
                     /locus_tag="PLM79_05400"
     CDS             complement(14356..14949)
                     /locus_tag="PLM79_05400"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOV85777.1"
                     /translation="MIVTLPDLPFAKEALEPHISAKTLDFHHGKHHKAYVDNANKLLE
                     GSELAKESVETIIEKTAGDPSKAGVFNNVAQAWNHSFYWKCMKPGGGGAPTGPIAQKI
                     DADLGGYQKFVEELKNAGMTQFGSGWAWLVLHNGKLQITKTANADTPLAHGMIPLLTV
                     DVWEHAYYLDYQNRRPDYLATFIEHLINWDFVNSCLK"
     gene            complement(15247..16590)
                     /locus_tag="PLM79_05405"
     CDS             complement(15247..16590)
                     /locus_tag="PLM79_05405"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOV85778.1"
                     /translation="MAANGELTELIEILKKAEQELATLEGGLQPPDKVLRKVRKAIME
                     LRAASARHGIKGLSGAAAKMAEYTDHPSLGPEQFMALNFALEALRRGLEERTLEAVRS
                     AIVEAMELLGFEPLELSARDRKETPGGSPNAPPSRRDPEAPSVPPLPESPAISGLNRV
                     ASSLGGKIETSLGSGGDSFKLEFPAACLEKIKFLLSPFDPEEDFSQVLPTQDERSNNI
                     LKSIKEFMASFAEGNLEHAQAVLEDLAGFQGEDELYREIGTLARVIHDSIRGLSQTLT
                     PDLRELVEDSIPDSENRLEHLLKLTEEAANTTLDHAEAIQNRIRQDLERLSKIEQHLA
                     RLKPIGEVAHERMDESSRILRELKESISANQEHLTTILTSQSYQDLTGQVINKVVEFQ
                     KDLESKLINLVRTFGVKSGGTRKQEKELYGPAHEKVAGAVHSQDEIDSLLAEFGF"
     gene            complement(16696..17988)
                     /locus_tag="PLM79_05410"
     CDS             complement(16696..17988)
                     /locus_tag="PLM79_05410"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOV85779.1"
                     /translation="MANVVVIGTQWGDEGKGKVVDLLTEKADCVVRFQGGNNAGHTLV
                     VEGTQYIFHLIPSGILHPGKVCMIGNGVVLDPGVLLQEMDRLNGLGRSVTPDNLVISR
                     YTHIIMPYHRALDLARENAKGHGKIGTTGRGIGPCYEDKVCRSGIRLHDLFDEQALRS
                     KLERNLKEKNFLLEHFFGASPLEVDAVAEEYLAYGRRLAPFADNVSERLQQSVRENRH
                     ILFEGAQGTHLDIDHGTYPYVTSSNTVAGNACCGSGIGPTRIHAVLGVVKAYTTRVGG
                     GPFPTELEDETGERIRAVGGEFGSTTGRPRRCGWLDMVVVNSAVRLNGLAGLIITKLD
                     VLTGIPKLKICVSYRCGTRSLDMVPPELEALEKCEPVCREFDGWEEDLRGIRRYQDLP
                     LNTRRYLEAVEELSGVPIGIVSVGPAREETILLKDPFQ"
     gene            18534..21221
                     /locus_tag="PLM79_05415"
     CDS             18534..21221
                     /locus_tag="PLM79_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85780.1"
                     /translation="MTRFLFSLRFRILLIVFLAVLPALLLVLRTGFELRRMAVAKARD
                     HALSLVHVASMDYKLLVQETHHFLIALAERSAVRNGDRARCSPLFAGLLRENTHYTNI
                     GLIDLDGRFVCSAIPAEHPVDVSDRSYFRRVLETRELVIGDFQVGRISGKASLTFAYP
                     VLDAEERVEAVVVADLDLAWLNDLGTNARLPDGATLTLLDSHGIVMAHYPNPQERIGK
                     SFYENGVKDLFPAEGERVAEVRGSEGRHRFYACTDLAAPHQGTVCLGIPKETILSEAD
                     YILRENLVAVGGAGLLALIAAWGLGQFFMMRKIDVLVDTSRRLAEGDLGHRTGIAPGR
                     SEIRQLAQAFDEMADRLQLRREERDRMEDALRQSEAKFRTLVEQIPAITYVASLDSQS
                     TSLYVSPQSQTILGFDEEDYRADPDLWRKRLHPEDRDRVMEELARCHADNELFVSEYR
                     MIAKDDRVVWFRDEARVVRDESGLPLFLQGVMVDITEQKRTSEALRESEALAGIILEN
                     ISDTVFITDDAGGVTYVSPGTGGIFGLSPQEVYALGNISRLLGSDLFDPDALKESGEL
                     QDIEREVVDGRGKKHALLVNVKRVNIKGGTVFYTCRDISVRKRMENALRRARDELELR
                     VEQRTAELAALNEELRGEILTRRRAEEAVREAMEKLKFFAYSVMHDLKSPSIAVHGLT
                     NLLKKRCSNAMDEKSMLFCDQIIKASEHVAALVDKINVFIATKESPLVLEAVSTREIF
                     GTLMEEFSAKLTRRRVSWVQPEEVVEVRADRMLLLRALRNYVDNALKYGGERLSEIRI
                     GYEESESVHIFSVSDNGEGVTGKDSEKIFEMFHRNHTSRGIEGAGLGLAIVREIAEQH
                     GGKVWMRPRSDQGSIFYLSIAKDLPDKDTPAARAWAGNG"
     gene            complement(21343..22200)
                     /gene="htpX"
                     /locus_tag="PLM79_05420"
     CDS             complement(21343..22200)
                     /gene="htpX"
                     /locus_tag="PLM79_05420"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HOV85781.1"
                     /translation="MDSRFRTTVLLAVLTLLIVWIGHFFGGSNGMIIALMLAALMNLG
                     SYWFSDRLVLAMYRAQPLDQREAPDIYRMVRELAAEANIPMPRIYWIPEETPNAFATG
                     RNPENAVVAVTEGLWRLLTPEEIRGVLAHEIGHIKNRDILVSSIAATLAGAVMVLADM
                     ARWGAIFGGFRGSDDEDHGGGILGFLVMAVVAPIAAMLIQMAISRSREYLADETGARL
                     SHRPESLARALEKLSLASHQIPMEDAKPATAHLFIVNPLSGKSLVNLFSTHPPIEDRI
                     RRLRSMRRV"
     gene            22654..24399
                     /locus_tag="PLM79_05425"
     CDS             22654..24399
                     /locus_tag="PLM79_05425"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOV85782.1"
                     /translation="MRYSNYFIHTLFEVPKEAETPSHILLLRGSYIHPVAAGIYSLLP
                     LGHRTAEKVKAIIRDELDRIGGMEITMPVLNPAELWKKTKRYFDIGQELFRFKDRRDR
                     DFVLAMTHEEVVTDIARQFLKSYRDLPIMLYQIQTKIRDEARPRAGLLRVREFFMKDA
                     YSFHPDFSDLDGYYPRIFNAYLRIFARCGVKAVPIEADTGIMGGTGSHEFMLESPNGE
                     DRFVLCTRCDYRANTEKAVGLKPPPGDPPGEVPPMEKVPTPGIKTIAELMDFFGTGED
                     RFLKTVAYEADGELVLVAVRGDFAVSATKLANHLKAVHLEMASEDLLRSLGLYGGFLS
                     PVALSGVRLVLDTSVDDRSSYVAGGNEPDVHWKNVVYGRDFPRVDRVDVTEVREGDGC
                     AQCKTGILEIRRGIELGHTFKLGTKYTAENSMDVTYLNSAGEPQRVVMGCYGIGVERL
                     VAAVIEQHHDGGGMILPPTIAPFQVMLTSLGGKPDVVSTAEEIYRSIQSRFEVLYDDR
                     DESPGVKLKDADLLGIPVRVVVSGKWLRSGEVEIKVRKTGEVFPCARKDLEETLHRVI
                     RDLQPSLDGLPYLPE"
     gene            complement(24452..25816)
                     /gene="tilS"
                     /locus_tag="PLM79_05430"
                     /pseudo
     CDS             complement(24452..25816)
                     /gene="tilS"
                     /locus_tag="PLM79_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
     gene            complement(25813..26622)
                     /locus_tag="PLM79_05435"
     CDS             complement(25813..26622)
                     /locus_tag="PLM79_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85783.1"
                     /translation="MKTSPDSTPKRLRGVAALFDDFERKELFHKYLFFLGWIEILIFA
                     TCWFYQLSDRGSDPSGPVSAPFPWKVYFLVAFLAPVAITFLLGMIVVGFNKYFGETET
                     LGEAVFPDLPAEGNGEQAGRVYKLRQLVHWLQGLPFLGLLLLLGVAVGFFYKLDVLLA
                     FLSNVGEKSVKIVLISASVLVAILSVFALVLIFLNFQLRKKSMEYRYRSEMAERFGLI
                     ILEDNTVLNNEGKLISHGKKLLPLLPADTPPGGRQETSGPPLRPQSDPKPS"
     gene            26777..27679
                     /locus_tag="PLM79_05440"
     CDS             26777..27679
                     /locus_tag="PLM79_05440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOV85784.1"
                     /translation="MTDQVVFQTDLKGLKLLSRGKVRDIYDLGDTLLIVATDRISAFD
                     VVMPTPIPDKGRILTQLSAFWLEFLGRIVPQHLISTRVREFPSVCDPHAAVLAGRSMW
                     VRKADVLPVECIVRGYLVGSGWKDYEKTGTVCGLSLPPGLLEAQKLPETLFTPSTKAA
                     HGAHDENISFEEMGRMVGRDVARQVKDVSIALYETAASYAAEKGILLADTKFEFGLKD
                     GALMLVDEVLTPDSSRFWPADRYRVGTSPESFDKQYVRDYLVQSGWKPSDPAPQLPPE
                     VVANTRSRYLEALKRLTGRSLETL"
     gene            27676..28659
                     /locus_tag="PLM79_05445"
     CDS             27676..28659
                     /locus_tag="PLM79_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOV85785.1"
                     /translation="MTDRTLSPVRLEDMAWEDTTFAVPCYSSRDLLLESLGRNGILHP
                     PWVWDEPGGKYVIVDGFTRLRWARESGAESLPCTVFGRRTPRERLVCLRIEEKLLGSP
                     LNPAEKARLVALLGEISPVEKDFPRLLSALGISGRREIVEAWRRLDSAGAELLEAAAS
                     GGICERAALELARWDPGSAREVLGWFRVLRCSASIQVEVLDRIGEIAAREKREKLDLL
                     KDPAWGSILGSSHWNHREKTRELRNLLARRRFPRLHARMDRFRRDLAGLSLPESLQLV
                     PPRDFEGDRWELRLGFSTESDLARGAKAAQGLAESGKLDEVLHPDRFAGSP"
     gene            28668..29780
                     /locus_tag="PLM79_05450"
     CDS             28668..29780
                     /locus_tag="PLM79_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HOV85786.1"
                     /translation="MDGLVKPPRKVLVEAGLEDGELVRCLRSTLPRADFRVLEKISDR
                     SSFDPEVLEVVDFRGRFLKSCPGTRHYHCCGYQILHFGIQCTLGCSYCILQAYLNQPN
                     LQIFGNWEALFTEIEEKLRAQPSRLHRIGTGEFTDSLLLDPWTGFSRRLVSFFARQPN
                     AVLELKTKTNHVGNLRGLDHRRRTIAAWSLNAPYIRGREETAAASLRARLEAARACAR
                     WGYPLAFHFDPLVEYPRWKDGYARILDRLLDSADPGQIVWISLGCFRFMPSLRSAVLA
                     RHPRSGVLLGEFVRGLDGKMRYFRDIRVEMYSFMVERIRKADPGLCVYLCMEGEDIWR
                     EVFGFQPGEKGGLPAMLDRAARERTGIEDGASQKNP"
     gene            29900..31084
                     /locus_tag="PLM79_05455"
     CDS             29900..31084
                     /locus_tag="PLM79_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOV85787.1"
                     /translation="MDFQFTDEQRMMKETVYKWALKELGPLQEKIDEEDWFPPDFFKK
                     CAELGILGITISEEYGGLDGDILMQTLAVEEMSRICPALAMTYGTHSNLCANNIHRNA
                     SPALKKRYLPPLVAGEKIGALALTEPNAGSDAMGIRTRAVKKGDRFILNGSKMFITNG
                     PVADILLVYAKTVPEKGAKGISAFIVEKDFPGFFVSRSLKQCGMRGSPTGELVFDNCE
                     VPEENLVGELNMGVHVMTSGLDIERIVLAGGSIGMAQQALDYSIRYAVEREQFGQPIA
                     NFQMIQQKLADMFARTEAARLLLYRAATMAQESHRGGKGTELTKLAAAAILLASETAT
                     WVCGQGVQIHGGYGYCLEFPVQKLWRDAKLYEIGAGTSEIRRMIIARELTREEFARRR
                     QR"
     gene            31131..32084
                     /locus_tag="PLM79_05460"
     CDS             31131..32084
                     /locus_tag="PLM79_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85788.1"
                     /translation="MEYGLRRYEAAQRLFWILFFVLCLGVAYLFAEAAANSPNGSAAA
                     LPTFQPDSLKSLSFWLQLRWIRGEPFGKILVYLLYVALIFVGARFCWLLVQYAGKFYV
                     KGLLASHVRKPAASPKPSLEQLSAKPEKLFPVDILLKKSNPLFLRLLFHPMTRLRLLL
                     AKNQGTLSSEDLMERERRMVETDWQVLYGSWTPFRWLLWLLPLFALLQSSWLLYHQVQ
                     PAVTGQKEIHDVLGPVFASFIPLVQIILINVLLSLGSGLLKRMEGFYLSGVDTLFYDR
                     FLSRLPFQSSDTLILLEALNRHFQELHFVLRRLEKSLGDGK"
     gene            32101..32601
                     /locus_tag="PLM79_05465"
     CDS             32101..32601
                     /locus_tag="PLM79_05465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOV85789.1"
                     /translation="MEGKKSRDDPGIGQWIRKVLEKHPAFLANPVGDWNEIVGEQVAR
                     YTQPVSLKDKVLTVAAYDSVWKYHLEVLKEPILAKINRKRPIPLVEKILVRVGEVPEP
                     QPVLNRNHRLLSQIKGKRSRAGTAKKSPLRSLTPEEKEFLKRLPDNDLRKIGARLLRK
                     TPLEDE"
     gene            complement(32782..34728)
                     /gene="uvrC"
                     /locus_tag="PLM79_05470"
     CDS             complement(32782..34728)
                     /gene="uvrC"
                     /locus_tag="PLM79_05470"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOV85790.1"
                     /translation="MTEPDFLLTTPREQPLAEAVQALPHVPGVYLFKDCAGTILYAGK
                     ARDLKKRVSSYFRSPPSENIKTRVLLSKTASLEFAVTATEKEALLLEAGYIKKYRPRY
                     NVVLRDDKNYPALRLDLRDPFPRLEIVRRIQKDGALYFGPYPSAHAVRETVRFLHQLF
                     PLRLCKSKRLSRRERACLNHSMGRCPAPCVGNIDPEEYGRMVREVVLFLQGKTDALQR
                     QLQRSMEEASQSLEFERAAVYRDRLHAVRTTLEKQVIVSDRFLDQDVVGIHRDPDGIE
                     MAVLFVRQGVAAGQRHFDLKDAHGETEELLASFIQQFYGEDRFIPDEILVPGPFEGIE
                     VLEEWLRDLKGKRVHIRPARRGRGKQLLDLAEKNARERLQSRRKSEKRDRSLLEGLQR
                     ILRLPKPPLRIGCVDISNLQGRHAVGALAVFSDGRPHKESYRRYRIASKEEPDDPAMM
                     AEVIERLVTRERELADTLDLLMLDGGKGQLNRIVRVLSETGMADRLPVISIAKERGPD
                     RGEKGRGLYEKIYVPGRRNPLFLARFPDILHLLQRLRDEAHRFAVTHYQALHRRELLL
                     SALDAVPGIGVKRRRILLDHFGGMDSLREASLEELEQVPGIPPAVAKAIFDFLSAQSP
                     EASSRWDSPDGALDAAPPESPPTE"
     gene            34925..35001
                     /locus_tag="PLM79_05475"
     tRNA            34925..35001
                     /locus_tag="PLM79_05475"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34959..34961,aa:Pro,seq:cgg)
BASE COUNT         6692 a        10740 c        11089 g         7047 t
ORIGIN      
        1 tcgggaaact gccgaaccac ctccagggta ttcactccga tggagccggt actgcccaga
       61 atgctcagaa tctttgccac aacgtcctcg aacccgtcaa agttccggtg agatcggaag
      121 tgcccgggtg cttccctcgc cggcgagagt ccctgtgcta caagaacagc cagctggtgg
      181 gaaatacgaa aagaagactg tccaatcggt ccagaatgcc gccgtgaccg ggcaggagcc
      241 ggctggagtc tttctttccg ctgatcctct tcatcatgga ttcgatcaag tctcccgtct
      301 ggctgatgag tgccagggcg acgctgagca ccagcgtctg ggggatggtc ctttcgggca
      361 agagcagttg cccgattccc acccctgcca ggacgctcag aaggagtccc cccgcggaac
      421 cttcgacggt cttcttgggg ctgaccgctt catagagctt gtgccgtccc caggtccttc
      481 cggagtagta tgccccggcg tcgctacaga ccaccaccac cagggtgaaa aggagccacc
      541 gccttcccgg gggagcttga ccgatcaaca aaacatagga caaaagataa ggaatgtaaa
      601 gccagcccag ggtgcactgg gccagacgcc gaatcccgtt ggaatccaag ggagagcgag
      661 ccagcatgcc gagcagtgcc ccgaacaatc caaggatcag gccgcaatga agccccgtgg
      721 gtccgcccag ggcggctccc gcgggaagga acaggccgcc ggaatagtaa agcacccgcc
      781 attgccggga agaacgcacg tcggagacca gcaggtgctg aaattcccgc agacccagga
      841 gggccgcaag gagcaccatg accgtccagg tccagtagga cccggccgca atgacccaca
      901 aaacgggaac caggaccagg atggccgtaa cgagcctttg cgtgtgagaa ctcatggtct
      961 gccgcttcca catctttgga ctgttcagct cgccttttcc tggacctggt ccccggtctt
     1021 tccaaaacgc cgctctcgtc cttgaaagtc cagaagcgcc tcgatgaatt ccctttcccg
     1081 aaagtcgggc cagagggtgt tggtgatgta gaactccgag taagccgcct gccagaggag
     1141 gaagttgctg acccggtgtt ctccccccgt gcggatgatc aggtccgggt ccggcatccc
     1201 ggaggtgtac aggtatcgag caaaatccgc agggctgggg ggctgggcgg gaattgcacc
     1261 cccggccatt tcccgggcat aggtccacgc ggcctggacg atctcctggc gtcctccgta
     1321 attgatggcc aggctaagga tcaatttatc gaacgaggcg gtttgtgctt ggacggaata
     1381 gaggtcctcc aaaacatccg ggggaatccc ctggggatct cccaggtgcc ggagacggat
     1441 cccgttctcc agcagttccg gcagctcgga tttgagaaac cgcttgagaa gcagccagag
     1501 ggcttccact tcgtgggggg gcctttgcca gttctctttg gaaaaggcgt acagggtaag
     1561 gtaagggatc tcgagcttgc ggcaacagcg aacgatttcc cggacggacc ccgcgccttc
     1621 ttggtgcccg gtaatccggt tgagcagctt cagcttcgcc cagcgtccat tgccgtccat
     1681 gatgatggcc acatggcgcg gaagcttctt gagatccaga cgatccattc agatttccag
     1741 gatttctttt tccttgatgc cgtacactcc gtcgatcctc ttgatgaagt cgtcggtgat
     1801 cttctgaatc tcttcttgtg cgcggaattg ctcgtcttcg gaaatctctt tttctttctt
     1861 caactctttg atcatctcgt tcacgtcgcg gcgaatattg cgagcggcca ccttggcttc
     1921 ctccgccatc ttcttgatga ccttcaccag ctcccgacgg cgctcggcgg tgaggggcgg
     1981 gatatggatc cgaatgacct tcccgtcatt ggtggggttc agtcccagct ccgacttgtg
     2041 gatggccttc tcgacctcgc cgatgacggt gctgtcccaa ggttgaatca tgatggtgcg
     2101 gggatcggga acggtgatgg tggacagctg gtttaggggt gtcgggactc cgtagtattc
     2161 cgcccggatt tcgtccacca gggaaatcga ggccctgccc gttcgcagcc tcttgtattc
     2221 ccgctcgagg gtttccagtg ccttgttcat gcgatcccga gcatccgcgt agacatcatt
     2281 caacatgacc gactccctcc accacggttc ctatgggttc tcccaggatg acttttttga
     2341 tgttgcctcg ttgtttcagg ctgaaaacca cgatacgcat attctggtcc tttgccagag
     2401 aaatggcggt ggcatccatg acgcggaggt tctttcgaag gacttccatg tagctcacct
     2461 ggttgtattt caccgccatc gggtcctttt cggggtcgtc gttataaacg ccgtcgacct
     2521 tggtggcctt catgaggacc tgggcgccga tttccaccgc ccgcaaggcg gcggtggtgt
     2581 ccgtggtgaa gaaggggaga ccggttcctg cggcaaaaat caccacgcgc cccttttcca
     2641 ggtgccggat ggcccggcgc cgaatgtagg gctcggccac ctctttcatg tcaatggcgg
     2701 tcaatacccg ggtgggcatc ccgtgtttct cgagggcctg ttgcagggcc agggcgttca
     2761 tgaccgtagc cagcatgccg atgtagtcgg ccgtagaccg atccattccc tgggccgctc
     2821 ccgacattcc ccgaaagatg ttgcccccac ccaccaccag ggccacctcc acacccaatt
     2881 cccggacctc ttgaacctcc tcggcgatca cggtcaacac ttgggggtcg atcccatagg
     2941 agccggatcc catgagggct tcgccgctca gcttcagcag gatccttgtg taccgaggtt
     3001 ttgacgcgtg gctcatgcac cctcgcaaca ctcgaaatcc cggggctcgc tcggctccgg
     3061 ggatgaaggg gcgtctgccg tgagggccgc ggccgaaggt cggccggccg ccgtggcttg
     3121 ccggcagaaa gttcgggctc tactcctcgc ccaactggta tcgaacgaat cgacgcacga
     3181 agatcttttc gccgatcttg gctacgagtt cgttaagata gtcctgaatg gtcttttccg
     3241 gttccttgac gtaggcctgc tccatgagga cagattcctc gtagaacttt ctcatcttcc
     3301 cgtccaccat cttttccagg atgtgggcgg gctttccggt ctccccggcc tgggccaggt
     3361 agattccccg ttcccgttcg atgatttcct cgggaacgtc ttccttacgg actcccaggg
     3421 gattggtggc ggcgatttgc agggcgatgt tcctgacaaa ctcgacaaag tcctcgcttt
     3481 tggcggcaaa gtccgtttcg cagttcacct ccaccaggac cccgatgcgg ccccctccgt
     3541 gaatataggc atggaccatc ccttcctggg cttccttgcc ggatcgcttg agcgccttgg
     3601 ccaatccctt ccggcgaagg agatccacgg ccttttccat gtctcccccg gtgtcctgga
     3661 gagccttctt gcagtccatc atgcctgcat tggttttttc cctcagttct cgaaccatgg
     3721 aagctgtaat ttccaaaggg tttcctcctc tgtgttgtcg gtcttggcga aatcctattc
     3781 ctgctcttcc tgccgtgaca aggcgacggg attgacgatc tccacctcgg ggccgctggc
     3841 ttcttccttg aggatctgtc ccgcgggcac ttcttcgaag gccggttcct ctttgtcgcc
     3901 cagcgcttgc tgcttctcca gatgtcgctc tttgccctcg atgcaggcgt cggcaatgcg
     3961 ggaggtaatg agtctcacgg ctcggatggc gtcgtcgttt ccggggatgg ggtagttgat
     4021 gaggtccggg tcacagttgg tatccacgat ggccacgacc ggaattccca atcgattggc
     4081 ctcggccacc gcaatgtctt cccggtgggt gtccaccacg aaaagagcgc cgggcagccg
     4141 ggtcatgtcc ttgattcccc ccaggttctt ctccagtttc atccgttctt tctgcagttg
     4201 gagaatctct ttcttgggaa accggttgat gctgccgtcc tcgatcatgg cgtccagttc
     4261 tttgaggcgg tcgattcgca gcttaatggt cttgaagttg gtgagcatgc cgcccagcca
     4321 gcgctggttg acccaaaaca tgccggcccg ccgggcttct tccagaatgg tgtcgcgggc
     4381 ttgctgcttg gtgcccacaa acaggacgct ttctcccgct tcggcggtat cggcgacgaa
     4441 gcggaaggcg gtattgaaga gcttgacggt gcgctgcaag tcgatgatgt agatcccgtt
     4501 gcgggcgccg aagatgtaag gtttcatctt ggggttccat cggcgggtct gatggccgaa
     4561 gtggaccccg gcttccagca gttgtttcat ggtaacgacg ttcatagggt tctccttctg
     4621 gttggcctcc acccctcttc accgccaaca agcatcgggc accaaaaggg ttcgagaggt
     4681 gtgcggattt ggtaaatcgg gtgaatctaa catgaactct ccggtcgagc aataaaaaat
     4741 tcatggagag gatcctgcgg gacccgcagg ccgcccttct tccccatgct ctccaggacc
     4801 cgcaatttcc cccttgacat cccatcgagg cttttgctaa gagggagtaa ccctcaaaaa
     4861 ggaaaacgca tcatgagacg gagtttcgga caatccatat ttgcttggcg ctggtggtgg
     4921 aatgccagca gggaggggtg tgccgactcg gtcgcataag ctgaagcctc agcgttgagc
     4981 gccaaaaccc aaggaccgtg ggccactcca ggcccacggt cctttttgcg ttccggggat
     5041 cgtgggaaag ttcctgcgat ccccgttctt ttcttgggga ttccattttc cgcacgaggg
     5101 gtcgagggga tcttgccccg tcccccgtcg tccaaacgag gagtccagtc atgctggtcg
     5161 tcatggaaca ggaagctacg ccggaagaaa tcgaaagggc cgtccaggcc gtcgaatccc
     5221 gcggctttgc ggcccggccc gtgcccgggg ggcatcgggt ggccatctgc gtccttcata
     5281 accacgggtc cgtggaaccc tccctattcc tgggtttgcc gggggtcaag gaagtcattc
     5341 ccgttacccg tccctacaag ctggtgagcc gggagtccca cccgacggat accgtcgtcc
     5401 gggtggggga cgcctgcatc ggaaacggca ccctcaccct cattgccggt ccctgcgccg
     5461 tggagagcga gacccaggct ttgaccatcg ccgggctggt taagaaagcc ggggcccaca
     5521 tgtttcgggg cggggctttc aagccccgaa cctccccgta cgccttccag gggctgggag
     5581 aggacggcct gaagatcctt gcccgggtgc gccgggagac cgggttgccg gtagtcaccg
     5641 aggccatcga tcacgaagtc ttcgacctgg tggaagccta cgcggacgtg atccagatcg
     5701 gcgcccgcaa catgcagaat ttcagcctcc ttcggcgggc cggccgatcc tccaaacccg
     5761 tgcttctcaa acgcggtctt tccgccacca tcgaagagtg gctcatggct gccgagtaca
     5821 tcatggaagg cggaaacacc cgggtcatcc tgtgcgagcg gggggtgcgc acctttgccc
     5881 accacagccg caacaccctg gatctttccg cggttcccgt ggttcaaaaa gaaacccatc
     5941 tgccggttct cgtggatccc agccatgccg ccgggcgtcg aagccaggtg atacccttga
     6001 gccgggccgc cgtggccgtg ggggcccacg ggctcctggt cgaggtccat caccagccgg
     6061 aggcggccct cagcgacgga gcccagtccc tctatcccga gcagttcgtg gaactgtgcg
     6121 ggcagatccg ccccatcttc aaaatctttc gaccggatgg aatccattga tatggagcca
     6181 atttccacca aaccggaatc ggggttcaca ccatgagact gagacaattt cccgacaaga
     6241 acgaatttct ccgcatggcg gggagcagca acctcatccc cgtttgcctg gaggtcctgg
     6301 cggatacgga gacgcccctt tccgccctgg ccaaagtgta ccggggcaag ggaccggtct
     6361 ttcttctcga gagcgtcgaa gggggcgagc ggtgggggcg atacagcttc ctgggttctt
     6421 cggcccggtc ggtggttcga gtctacccgg accgggtggt gatcgagagc ggggcgtcca
     6481 cggaggaagt ccctcaccac gggcgcccct tggacgtgct gcgggccttt atggcgcgtt
     6541 tcaagcctgc atcccctgcg gggctgcccc ggttctgggg aggactggtg ggctacatcg
     6601 cctacgaagc cgtttccttc ttcgaggcca tcccccacga catgcccgtg gacgctcccc
     6661 tggctcattt cgccattccc ggcgagctcc tgatcttcga caacctccgg cacaccctga
     6721 ccctggtggc ttatgtgttc ctggaggaca cctctcccga ggccgcgtac catcgggcca
     6781 tcgagaagct cgatggaatg cgggccgtcc tggcgggccc catgccccac cgccccacgg
     6841 gatgccggga cttctgcgag ttgaaacccc acgtggaccc ggattccttc cgcggccagg
     6901 tggctcgaat caaggagtac atcgaggccg gagacgtcat tcagacggtc ctttcccagc
     6961 cgttttcctg ccaatatccc cccgatccgt ggtggcttta tcgcgcccag cgttacgtga
     7021 atccttcccc gtacctctac ttcctgcacc tggacgatat cgtcctggtg ggatcgtccc
     7081 ccgaggtcat ggtgcgcctc gagaacgggg tagccaccct ccggcccatt gcgggtacgc
     7141 gcccccgggg gaaaacggaa caggaagatc gaaaactggc cgacgagctc ctgcaggacc
     7201 ccaaggaaag ggccgagcac ctcatgctgg tggatctggg ccgcaatgac ctgggccgcg
     7261 tggccgaggt ggggaccgta caggtgacgg acctgctggt ggtcgaacgc tattcccatg
     7321 tgatgcacct ggtatccaac atctgctgcg acctcaagac ggggtgcgac gcgtgggacc
     7381 tgcttcaggc caccttcccc gcggggaccc tgtcgggagc gcccaaggtg cgtgccatgg
     7441 aaatcattgc cgagctcgag aaggagcccc ggggtccgta cggcggcgcc atcgggtacg
     7501 tgtcttttac gggaaacatg gatttggcca tcaccattcg cacggcctgc attcaaaagg
     7561 accgactggt ggttcgagcc ggggccggga tcgtggcgga ttccgatccg gaaaaggaac
     7621 ggctggaaac catcagcaag gccatggcca ttcagcgggc attgcagctc atttaccgag
     7681 gctgacaatc caggaccgcc cggcgaacgg gcccgaaaac agcatacaag agagggagaa
     7741 cgccatgttg gtcatgatcg acaattacga ttccttcacc tacaacctgg tgcagtatct
     7801 ccgggtgttg ggcgccgaag tccgagtggt gcgcaacaat gtggtttccg tggaggaaat
     7861 ccgggaatgg aagccccggg gaatcgtcat ctccccgggt ccctgccgcc cggaaaacgc
     7921 cgggatttcc gtttccctca ttcgcgagac cacgggaaag ctccctatcc tcggggtttg
     7981 cctggggcat caggccatcg ccgtggcctt cggcggaacg gtggttcgtg cccggcagct
     8041 catgcacggg aagacctccg ccatccagtc cgacggcaag accatctacc ggggaattcg
     8101 cagtcccttc caggccatgc ggtaccattc cctggcggtt cgcagggagg atctgccgtc
     8161 ctgtctcgag gtcacggccg agtccgagga cggggaaatc atgggaattc ggcaccggga
     8221 gcatcccacc gagggaatcc agtttcaccc cgagtccatc atgactcccg tggggaagcg
     8281 gctcctcagg aatttcctcc aacgagtgga ggggtagaac ctccgtaccg gggaaaggaa
     8341 gatcatgctt cacgaaagtc tcaagaaagt ggttcagcga caggatttgg acgagacgga
     8401 gatggccgag gtcatgaacg acatcatgac gggaaatgcc accaacgccc aggtgggtgc
     8461 cttgatggcc gccctggcca ccaagggcga aacctattcc gagctggccg gagcggcccg
     8521 gaccatgcgc cgcaaggcaa cccgcatcca gactccggcg gataccgtgg tggacacctg
     8581 cggaaccggc ggcgaccgga aggggacctt caacatctcc accacggcgg cctttgtggt
     8641 ggccgggtgc ggggttaccg tggccaagca cggcaatcgt tcggtctcga gccgatgcgg
     8701 cagcgcggat cttttggaag ccttgggggt gaggctggac ctcgaacccg agctggtcga
     8761 ggaagcagtg caaagcctgg gcatcgggtt tctcttcgcc cccctcttcc acggggccat
     8821 gcgttatgcc gcccaggccc gaaaggaagt ggggattcgc tccatcttca acatgctggg
     8881 ccccctcacc aatcctgcgg gggccaactg ccagttgttg ggcgtctacg ccccccagtt
     8941 gacggagatg ttcgcccagg cccttctttc cctgggaact cggagcgcct acgtagtcca
     9001 cgggctggac ggcttggacg agatttccgt gtgcgccccc acccggatct ctgagctcaa
     9061 agacggactc attcgaacct acgaggtgtc gcccgagcag ctcttggggc gtcgtgccga
     9121 acccgaagaa ctcctgggag gggatgcccg ggagaactcc cggatcaccc gggaagtcct
     9181 ggggggggcc aagggggcca agagggacgt ggtggtgctc aacgccgccg ccgccctcat
     9241 ggctgcaggc aaggctccgg acttctcccg ggggattcgc ttggccgagg attccatcga
     9301 cagcggggcc gccatgggga aactggaggc cctggtgcga ttcaccagcg agaacgctca
     9361 atgagacaag cagcgaatgg aatgacatga aaccggacat tcttcacaag atcctggaga
     9421 gaaagcgcca agaggtggaa acggcgcgaa gggctttgcc cgaagccgtt gtccgtcggc
     9481 tggccgagga acgcccagaa aggcgtccct tttttcagcg gctccaatcc cccctccccc
     9541 acggcgtgaa catcatcgcc gaaatcaaac gggggtcccc ctctcggggg atccttcgag
     9601 gagacctgga tcccgcgcga caggcgaagc tttaccaaaa gggcggggcc gcggccgttt
     9661 ccgtcctcac ggacgcttcc tttttccacg gaagctcccg tgacctgaag gaagcccggt
     9721 ccgccgtgaa ccttcccatc ctgcgcaagg atttcatcct ctccacctac caggtctatg
     9781 agtcggcggc catgggggcc gatgccgtac tgctcatcgt ccgcgccctt tcccgggaat
     9841 ttctccggga ctgcctctcc ctgtgcgggg agcttctcct ggatgccctg gtggaggtcc
     9901 attcccagga ggatctcgag acggcaacct gggccggagc ttctctcatc gggatcaaca
     9961 accgggacct ggggaccttt cgcacggacg tggaggtgtc cgtgcgcatg gcggcaagac
    10021 tcgatccccg gcaggtgggg gtggcggaaa gcggaatcga aaatcgcggc caggtggagc
    10081 gggtcctggg agcgggaatc tggaactttc tggtcggaga aagcctggtt cgagcggaaa
    10141 accccgtgga gctcctgggg cggctcctgg gacgcccgaa cgggaaagga tttccgggtc
    10201 gtgcccaaaa ggaaggtttt ctgaaatgaa ttcacagcag atgtcgggaa cggttcgaaa
    10261 aaggggcgcc cccccgtttc cccaggtgaa ggtgtgcggg ttgaaaagcc ccggggaagc
    10321 cgccgagtgc gcccggctgg gggcggatgc cgtcgggctc gtgttttacc ctcccagccc
    10381 ccgctttgtg acggatgaac aggcgcggga gatctgtggg gatctgccgg gttccgtcac
    10441 ccgggtcgga gtgtttgtcc aggaaggcta cgactccatc ctgcggcgga tcgagtcctg
    10501 cggaatcaac gcggtccagc tgcacggcgg cgaagacccg gaactcgtcc gccacttgac
    10561 tcgggaagga atcctggtga tcaagaccct ctttgcaaac gggacccccg gaatcgagga
    10621 ttcggcttcc tatgcggcgt cggcgttcct catcgagtgc cggggcggtc cccttcccgg
    10681 gggaaatgcc cggacctggg actggtctcg ggcggcgcct ctggcagggc gatatcccct
    10741 cgtcctggcg ggcgggcttg cccctgaaaa tgtttccgac gccatgtccc gggccctgcc
    10801 cgatgccctc gatgtgagtt ccggggtcga aatctcgccg gggagaaaag accttcaaaa
    10861 agtccgtgaa tttctggagg cggtcaggag cacgccatct tccagaaccc ttaggaggat
    10921 tttttcatga agccgaatcc acaatcccgc gttcccacct ttcccgaccc tcacggccac
    10981 ttcggccctt acggcggccg ctacgtggcc gagaccctca tgcccgccat cctcgaattg
    11041 gaggaggcct acgaacgaat tgcaggccgc cgggattttc acgaggaact ggggaaactg
    11101 ctcaaagact acgccgggcg ccccacccct ctgttctttg ccggaaggct cacggccaaa
    11161 ctgggggggg tccggatcta cctcaagagg gaggacctgg cccatacggg agcccacaag
    11221 atcaacaaca ccctggggca ggctcttctt gcccggtgga tgggaaaacg caaggtgatc
    11281 gccgagacgg gggcgggaca gcacggagtg gccacggcca cggcggcggc cctgttcggc
    11341 atggaatgcc gggtcttcat gggaaccgaa gacatcgagc ggcaggcggc caacgttcaa
    11401 aggatgaggc tcctgggggc cgaagtggtc cccgtacgct cgggcacggg caccctcaag
    11461 gacgccatga atgaagccat gcgctactgg gtgggagcgg tgcgggacac cttctatgtc
    11521 attgggtccg ttgccggacc ccatccctat ccccggatgg tccgggattt ccagagggtg
    11581 atcggggacg aaacccggga gcagatcctg gccgccgaag gccgcctgcc cgacgtgctg
    11641 gtggcctgtg tggggggcgg ctccaacgcc atggggctct tctacccttt tctcgaggac
    11701 gccgtggagt tggtgggagt cgaggcggcc ggtcgcggga tccccacggg caaacacgcc
    11761 gccaccctgg gagccgggtc cgtaggcgtc cttcacgggt ccaagtccta cgtccttcag
    11821 gacaaggacg gccagattct cgaagcccat tccatttccg caggactgga ttacccgggg
    11881 gtcggtccgg aacatgccca cctcaaagac atgggcaggg cccggtacga atccgtgacg
    11941 gacttcgaag ccttggaagc tttccgggaa ctctgtctgt gtgaaggcat catcccggcc
    12001 ctcgagagct cccatgccgt cgcctgggcc ctgcgggaag cccccaggcg cccgccctcg
    12061 gaggtgattg tcgtcaatct ttcgggcagg ggcgacaaag acctgggaat cgtggcaggc
    12121 aggctcgacc cccatcagcc ccaatgaatc ggaacggaaa ggagccacca tgaatcgaat
    12181 ccgaaagacg tttcaggacc tcgaggggcg aagggaaaag gccctggtgg gttttgtcac
    12241 cgccggagat ccggatattg cccggtcccg ggagctgatc ctgtccatgt gccgggcagg
    12301 cctcgacatc ctcgagctgg gagtgccgtt ttcggacccc acggcggacg gtcccgtcat
    12361 tcagcgatcc tcggcccggg ccctcagagc cggagtgaat ctcgcagcgg tccttgagct
    12421 cacccgggat cttcgtcgag aggtctcggt acccatcata cttttcagct attacaaccc
    12481 tatcctggcc tacggggcgg atcgtctgta cggggacgcg ctggccgccg gggcggacgg
    12541 cttgctggtg gtggatctgc ctcccgaaga atcccaagag atgacctccc ggtggccggg
    12601 ccgggatttg tgtctcattc gtctttgcgc tcccaccact cctccccggc gcatggaatc
    12661 cattgcccgg ggggcatcgg gctttctgta cctggtttcc atgacggggg tgaccggaag
    12721 cggcgggctg gatctcgagg agatttcccg agtggcgacc ttgcttcgga cctgtgccga
    12781 tgggactccc atctgtgtgg ggtttggaat ttccactccc gagcaggtga gagcggtggc
    12841 ctccgtggcc gacggggtcg tggtgggcag cgccttcgag cggttcatgg aatcgggcct
    12901 cgaggacccc gacctggggg agaagctggg agcgatggtc cgggggctca aggcggcaac
    12961 ccggttctag aggaaagcct cgggaaacgg ggatgcagcc ggacccagcc gtcctgtgcg
    13021 ctcgggcgag ccgacgccga cccctcgagg cgggcatttg gcccccccaa agcccatgtt
    13081 cacgaccggg gatctgtggt aaagaatgct tgtgacggat gccgagaccc gggggaacga
    13141 atcacacgga gagaaggttg cgatgttctt cttcataggc ggggtccaac caaagacggt
    13201 gaccctggag gacactgccc ggctttgccc cgcttgcggg ctggcccagg cgcggttgaa
    13261 acggatcgac cactatgtga gcctcttttt tattcccctt tttccggtaa agagggggga
    13321 ccccgtggtg atctgcgatc gatgcggagc cgtgtcggct ccccatccgg gggtggggga
    13381 ggtcccggtc gttctgaaag gaggcaactg ccttcgatgc gggtacccgc tggatccaag
    13441 ttttcggtat tgtccccagt gcggtgtccg ggtataaagg gggcggaaat ccattgaacc
    13501 gcggatcgga tgggtgagcg aaatgccgaa cgagaacaaa atactctttt tccccatggt
    13561 tgcccggccc tgtatcctgg aggtgctcac ggagtttctc gacgagcagg ccaaacgcct
    13621 gaaaccgagg acctaccggc gatacgagga ggtcgtcgac ctgtttcgac actgcatgaa
    13681 caactatggg gtggacgaac tgaccaattc cgccgacatc gtcttttacg agcggcttta
    13741 ctaccaaaag aatctcgaat tcaccgccat ttttgggcct gacaaaatct tgccgtgcct
    13801 tccctacttt ctcaacttct tcatgttgcg aaaggtgatg gcttcggagg gactgctcga
    13861 ggctgcgggg accgtgtccc ggcgcctggt gcggtggctg ctggaaaacg gctacgtcaa
    13921 agcggaggag gcggaagagg ccgccaaggt ggtgatggag gccgcccggg agcttcctgc
    13981 cgcggaacgg gtggcgaggc tgctgttcca ttatgcccag agtcatactc cccggtactg
    14041 gaccttggaa ctcgacgatt atttcaccat cgacaaggtg aaccccggga ccctggttct
    14101 cgccgggtcc aacacctccg ccctcgtgga gctgaaagtg cccaaagaga tcacggacca
    14161 gtgcaaggaa ggctggcaaa tcagcctgct tctgggaaag acccagggcg gctggtccat
    14221 tctcgagacg ggaaacgttt atccttcctg atctttgcgc caccctgatc tttgcaccat
    14281 atgaaagggg cggacccgtg cggcccgccc ctcttcgatg aaagacccct ctgccgatcc
    14341 ttcttcggca aaaacctact tcaagcagga attgacaaaa tcccagttga tgaggtgctc
    14401 gatgaaggtt gccaggtagt ccggccttcg attctggtaa tccaggtaat aggcatgctc
    14461 ccacacatcc acggtcagga gggggatcat gccgtgggcc aggggggtat cggcgttggc
    14521 cgtcttggtg atttgcagct tcccgttgtg gagaacgagc caggcccagc cgctgccgaa
    14581 ttgggtcatg ccggcgttct tgagctcctc gacgaatttc tgatagcctc ccaggtccgc
    14641 gtcgatcttc tgggcgatgg gtcccgtagg agctcctcct ccgccgggct tcatgcattt
    14701 ccagtaaaag gaatggttcc atgcctgggc cacgttgttg aatacgcctg ctttggaggg
    14761 atctccggcc gtcttctcga tgatggtttc cacgctttcc ttggccagct ccgagccctc
    14821 caatagcttg ttggcgttgt ccacgtaggc cttgtgatgc tttccgtggt ggaaatccag
    14881 ggtcttggcg ctgatgtgcg gctccagggc ctcttttgca aaaggcaggt caggcaacgt
    14941 cacgatcata actcctcctt gttcgctaga gtctttgggg ggtttaggga atgactatcc
    15001 gactacaccg gaaatgctga gatccgaacc gggtggctgg ggaagctgcc gtcgtccgcc
    15061 gatggtccgc gcagacggcc gaagggccgg aaaccctcgc caacgaaacg tttgataagc
    15121 atcggtcgga atgaattcaa gcgcgagcgc cgattatttt ggctcccccg gcctgccggc
    15181 gacggttcga ccttcccgtc cggtccggag gggaaggcct cgatagggat gaagaaggga
    15241 tgaccctcag aagccgaatt cagccaggag agagtcgatc tcgtcctggg aatggaccgc
    15301 ccccgccacc ttctcgtggg cgggaccgta gagttccttt tcctgcttcc tggttccccc
    15361 ggatttcacc ccgaaggttc gaaccaggtt gatcagcttg gattcgaggt ccttttgaaa
    15421 ctccacgacc ttgttgatta cctggccggt gaggtcctgg tagctctggg atgtgaggat
    15481 ggtcgtcagg tgctcctggt tggcggaaat ggattccttg agctcccgga gaatccgcga
    15541 gctttcgtcc atgcgttcgt gggcgacttc tcctatgggt ttgaggcggg cgagatgttg
    15601 ctcgatcttc gagagccgct ccagatcctg tcggattcga ttctgtatgg cttcggcatg
    15661 gtccagggtg gtgttggccg cctcttcggt gagcttgaga aggtgctcga ggcggttttc
    15721 cgaatcggga atgctgtcct cgacgagctc cctgagatcg ggggtcaacg tctgggacaa
    15781 accccggatg gaatcatgga tcacccgggc cagggtgccg atctcccggt acagttcatc
    15841 ctctccctgg aacccggcca ggtcctccag gaccgcctga gcgtgttcga ggtttccctc
    15901 ggcaaaagac gccatgaact ccttgatgga ttttaggatg ttgttggagc gctcatcctg
    15961 ggtgggaagg acctgggaga agtcctcttc cgggtcaaaa ggagacagga gaaacttgat
    16021 cttttccagg caggcggccg gaaactcgag tttgaaggaa tccccgccgg aaccgaggga
    16081 ggtttcgatc tttccgccca ggctgcttgc cactcgattg aggccggaga tggccgggga
    16141 ttcggggagc ggcggaaccg acggcgcctc cgggtcccgg cgggacgggg gagcgttggg
    16201 agacccgccg ggcgtttcct tccggtccct ggccgaaagc tcgaggggtt caaaccccag
    16261 cagttccatg gcttccacaa tggcggatcg gacggcctcg agagtgcgct cctccaatcc
    16321 gcggcggagg gcttcgaggg cgaagttcag agccatgaac tgttcgggac ccagggacgg
    16381 gtgatccgtg tactcggcca ttttggccgc ggcccccgag agtcccttga tgccgtggcg
    16441 agcggaagca gcccgcagct ccataatggc ctttcgaacc tttcgcaaca ctttgtcggg
    16501 aggctggagc cccccttcca aggtggccag ttcctgttcg gccttcttga gaatctcgat
    16561 gagttcggtc aactcaccat ttgccgccat gtcgcatcct tcatcgtagt tgcggggacg
    16621 ccgccctttc cgaacggata acccggccgg gcgggcagtg tttacgaacc ggaggccggg
    16681 tcccggtcgg gtctcttact gaaagggatc tttcaataga atggtttctt cccgggcggg
    16741 tcccactgag acgatcccga tgggaacgcc ggagagctcc tcgacggctt ccaggtagcg
    16801 gcgggtgttg aggggaaggt cctggtagcg gcgaatcccc cgaaggtcct cctcccagcc
    16861 gtcgaattcc cggcagacgg gctcgcactt ttccagggct tccagttccg ggggaaccat
    16921 gtccaggctt cgggtgccgc aacggtagct cacgcaaatt ttgagcttgg ggattcccgt
    16981 caacacgtcc agcttggtga tgatcaagcc cgccagaccg ttgagccgga cggcggaatt
    17041 caccaccacc atgtcgagcc agccgcaccg tcggggtcga cccgtggtgg agccgaattc
    17101 ccctcccacc gctcgaatcc tctccccggt ttcgtcctcg agctccgtgg ggaaaggacc
    17161 gccccccacc cgcgtggtgt aggcctttac gacccccagg acggcgtgga tccgggtggg
    17221 gccgatgccg gatccgcagc aggcgtttcc ggcaaccgta ttggatgagg tgacgtaagg
    17281 ataggtgccg tggtctatgt caaggtgagt gccctgggcc ccctcaaaaa gaatgtgtcg
    17341 gttctctcta accgattgct gaaggcgctc ggagacgtta tcggcaaagg gggccagtcg
    17401 acgcccgtag gccaggtatt cctcggcgac ggcgtcgacc tcgagggggc ttgccccaaa
    17461 gaaatgctcg agcaggaagt tcttttcctt caggttgcgt tcgagcttgc ttcgcagggc
    17521 ttgctcgtcg aaaagatcgt gaagacggat tcccgatcgg cagactttgt cttcgtagca
    17581 gggtccgatt ccccgaccgg tggtgccgat ctttccgtgc cccttggcgt tctcccgggc
    17641 caggtccagg gccctgtggt acggcatgat gatgtgggta tagcggctga tgaccaagtt
    17701 gtccggagtt acggaccgcc cgaggccgtt caaccggtcc atttcctgga ggagcactcc
    17761 ggggtccagc accaccccgt ttccaatcat gcacactttg ccggggtgga ggattcccga
    17821 cggaatcagg tggaaaatgt actgggtgcc ttccaccacg agggtatggc cggcgttgtt
    17881 gcccccctgg aaacgaacca cgcaatccgc tttttccgtg agcagatcga cgacttttcc
    17941 tttcccctca tccccccatt gagtcccgat gaccaccaca ttcgccataa tgctctcttt
    18001 caaaaagaaa cgaatcgaac gggctttcca cacggtcgtt ccataggttg aacccctttt
    18061 cccgggcacg attttccggc gttggccgtt gcgcccaacg tcccgcaaat cccgggattg
    18121 tccccgtccc gacggggtgc gcaatggcga tcgggctgtg ccggggtcga tcctcccgga
    18181 aatcccggta tggcacccgg ggacccgcag agggcctctg cccgaaacac cagcgttacc
    18241 tgtaatgaaa aaaacgactt gtgtcaaagg gtaatccaaa acccgtccgg gcgcgccggg
    18301 tctcccccgg ggccgcctgc ccccgagctt tccgtccttg acatcctgcg gtttcttgcg
    18361 tagtgtgcag gcatcgcttg gcaagatcct gaggtaggga ccacaacgtt tcatccattg
    18421 ccgattttag ccgcacaaga atccaacgct cgacgggtct gaacgatcaa gcatccccgt
    18481 ggccgggccg taggtcttct caggcgtacc ggggaggaaa gggaaagttc cagatgactc
    18541 gtttcctgtt cagtctccga tttcgcatcc ttctcatcgt gtttctggcc gtactgcctg
    18601 ctctgctgtt ggtgttgcga accggcttcg aactgcgacg tatggccgtt gccaaagccc
    18661 gggaccatgc cctgagcctg gttcatgtgg cctccatgga ctacaagctc ttggtccagg
    18721 agacgcacca tttcttgatt gctcttgcgg agcgttccgc agtgcgcaac ggagaccggg
    18781 cccggtgttc tcccttgttt gcaggactgc tccgggaaaa cacccattac accaatatcg
    18841 gtctcatcga cctcgatggg agattcgtct gcagtgccat cccagccgaa catcccgtgg
    18901 acgtatcgga ccgctcttac ttccggaggg tcctcgaaac ccgggagttg gtcatagggg
    18961 atttccaggt gggccgcatc agcggcaagg catccctcac cttcgcgtac ccggtcctgg
    19021 acgccgagga acgggtggag gcggtggtcg tggccgacct ggatctggcc tggctcaacg
    19081 acctgggaac caacgcccga ttgcccgacg gggcgaccct gaccctgctg gattcccacg
    19141 ggatcgtcat ggctcattac ccgaatcccc aagaacggat cggcaagtcc ttttacgaaa
    19201 atggggtcaa agacttgttc ccggccgaag gggaacgggt ggcggaggtc cggggttccg
    19261 aaggaaggca tcgtttctat gcctgtacgg acctggcggc tccccaccaa ggaacggtct
    19321 gcttgggaat ccctaaagaa acgatcctgt ccgaagcgga ctacatcctg cgcgaaaacc
    19381 tggttgccgt cgggggcgcg ggactcctgg cgctgattgc cgcctggggg ttggggcagt
    19441 tcttcatgat gcgaaagatc gacgtcctgg tggacacttc ccggaggctg gccgagggag
    19501 acctgggtca ccgaacggga attgcgccgg gaaggagcga aattcgccag ctggcccagg
    19561 ccttcgacga gatggccgac aggctgcaat tgcgccgaga ggagcgggat cgaatggaag
    19621 atgccttgag gcagtccgag gccaagttcc gcaccctggt ggaacagatt cccgccatta
    19681 cctacgtggc ttccctggat tctcagagca ccagcctcta tgtgagccct caatcccaaa
    19741 cgatcctggg cttcgacgag gaggactacc gtgccgatcc cgatctctgg agaaaaaggc
    19801 ttcaccccga ggaccgggac cgggtcatgg aagaattggc tcgctgccat gccgacaacg
    19861 agctctttgt ttccgaatac cgcatgatcg caaaggatga ccgggttgtg tggttccggg
    19921 acgaggcccg ggtggtgcgg gatgaatcgg gactgcccct gttcttgcag ggagtgatgg
    19981 tggacattac cgagcagaag cgaacttccg aggccctccg ggagtcggaa gccctggccg
    20041 ggatcatcct ggaaaacatc tccgacaccg ttttcatcac cgacgatgcg gggggggtca
    20101 cctacgtttc cccgggcacc gggggaattt tcgggttgtc tccccaggaa gtctatgccc
    20161 tgggcaacat cagccgcctc ctgggctcgg acctctttga tcccgacgcc ttgaaggaat
    20221 cgggagaact ccaggacatc gaaagggagg tcgtggacgg gagaggaaag aaacacgccc
    20281 tcctggtgaa cgtgaagcgc gtgaacatca agggtggaac ggttttctac acttgtcgcg
    20341 acatctccgt gcgcaagagg atggagaatg ccctgcggag agcccgcgat gaactggaac
    20401 tgagggtgga gcagagaacc gcggagttgg cggcgctcaa tgaggaactg cggggggaga
    20461 tcctcacgcg ccggagagcc gaagaagccg tgcgggaggc catggaaaag ctcaaattct
    20521 tcgcctattc cgtcatgcac gacctcaaga gcccgtccat cgccgttcac ggcctcacga
    20581 accttcttaa aaaacgctgc agcaatgcca tggatgaaaa gagcatgctc ttttgcgacc
    20641 agatcatcaa ggcatccgag cacgtcgccg ctctcgtgga caagatcaac gtcttcattg
    20701 ccaccaagga aagccccctg gtcctcgagg ccgtcagcac ccgggagatc ttcgggaccc
    20761 tcatggaaga gttctcggcc aagctcaccc gtcgccgggt ttcatgggtc caaccggagg
    20821 aggtcgtcga ggtccgggcg gatcgaatgt tgttgcttcg ggccctgagg aactacgtgg
    20881 acaatgctct gaaatacggc ggggagcggt tgagcgaaat ccgcatcggg tacgaggagt
    20941 ccgaatcggt ccacatcttt tccgtcagcg ataacgggga aggggtgacc gggaaggatt
    21001 cggagaagat cttcgagatg ttccaccgga accacacctc ccgggggatc gagggagcgg
    21061 ggctgggtct ggccattgtt cgagagattg cggagcagca cggcgggaag gtctggatgc
    21121 gcccccggtc cgaccagggc tccatattct acctatccat cgccaaggat ttgccggaca
    21181 aagacacgcc tgccgcccgg gcgtgggcgg ggaacgggtg aggatcgccc cgggagggga
    21241 agccatcctc ccttcccggg cgaaacggta tggcaccggg tgatcgatct gcccgggaac
    21301 cgatttgaaa gacctttata ggccttggcg cgccgggctt tctcagaccc ttctcatgct
    21361 gcgcagcctg cggatccggt cctcgatggg cggatgagtg ctgaaaaggt tcaccaaact
    21421 ctttcccgaa agcgggttca cgatgaaaag atgcgccgtg gcaggcttgg catcctccat
    21481 gggaatctgg tgggaagcca gggacagctt ctcgagggcc cgagccaggg attcgggtcg
    21541 atgggacagt cgggcccccg tttcgtcggc caggtactcc cgggaccggg agatggccat
    21601 ttgaatgagc atggccgcga tgggagccac gacggccatg acgagaaatc ccaggattcc
    21661 tcccccgtgg tcctcgtcgt cactgccccg aaatcctcca aagatcgctc cccatctcgc
    21721 catgtcggcc agtaccatga cggctcccgc cagagtggcg gcaatggaac tcaccaaaat
    21781 gtcacgattc ttgatgtgcc caatctcgtg ggccaggaca ccccggattt cctcgggggt
    21841 cagcagccgc caaagccctt cggtaacggc cacgacggcg ttttcgggat tccggccggt
    21901 agcgaaggcg ttgggggttt cctcgggaat ccagtagatc cggggcatgg gaatgtttgc
    21961 ctcggcggcc agttccctca ccatacgata gatgtccggg gcctcgcgct gatccaaagg
    22021 ttgagcccgg tacatggcca gcaccaggcg gtccgaaaac cagtagcttc ccaggttcat
    22081 aagggccgcc agcatgaggg cgatgatcat cccgttcgag cctccgaaga agtgcccgat
    22141 ccacacgatg agcaaggtca gcacggccaa cagaacggtc gtacggaaac ggctgtccat
    22201 cttattttcc ctcctgacaa aaaaaccgat cgattcaagg gtcttggagt cacaaccggg
    22261 taatatctcg tgctttttca aatttcatcc ccaacttaac caagggcctc ccgagtgtca
    22321 aggcaaagcc cccggggcat ccgtctcgat ccctccggcc atccgtccgg atcccggtga
    22381 tgtttttcct tccctgcttc gaaccccggg cccctcgatt cggcggcgaa tccgtcatcg
    22441 gtccgggcac cctcggtctc cggggcattt ccggacattt gggattgtgg acttcggaaa
    22501 agacgtgcta ttagaaggcc ttgccgggtt cacggaaccc cgcggaattc ttcgggggcc
    22561 gggcaaatcc aacggtccgg gaacgggcgc aggaagggga actccacccc ccctcgatcc
    22621 acaggtcgtt tcaccaacgg gagtacagcg gccatgcgtt attccaatta tttcatccac
    22681 accttgttcg aggtgcccaa ggaagcggaa accccttccc atatcctgct tctccggggt
    22741 tcctacatcc acccggtggc tgcgggaatc tactccctgc tgcccttggg acatcgcacc
    22801 gccgagaagg tcaaggccat cattcgagat gaactggacc ggatcggagg catggaaatc
    22861 accatgccgg tcctgaatcc cgccgaactt tggaagaaga ccaagcggta tttcgacatc
    22921 ggccaggagc tctttcgctt caaagaccgc agggaccggg attttgtcct ggccatgacc
    22981 catgaagaag tggtgaccga tatcgcccgg cagttcctga aatcctaccg ggatctgccc
    23041 atcatgctgt accagatcca gaccaagatc cgggacgagg ctcgacctcg agccgggctg
    23101 ctccgggttc gcgagttctt catgaaggat gcctacagtt tccacccgga cttctcggac
    23161 ctggacggct attacccccg cattttcaac gcctacctgc ggattttcgc ccggtgcggc
    23221 gtgaaagccg ttcccatcga ggcggacacg ggcatcatgg ggggaaccgg ctcccacgag
    23281 ttcatgctcg aatcccccaa cggcgaggac cgtttcgtcc tgtgtacccg gtgcgactac
    23341 cgcgccaata cggaaaaggc cgtggggctc aagcctcctc ccggggaccc gcccggcgag
    23401 gttcctccca tggaaaaggt ccccaccccg ggaatcaaga ccatcgccga gctcatggat
    23461 ttcttcggga ccggggaaga ccgatttctc aagacggtgg cctacgaggc cgacggggag
    23521 ctggtcctgg tcgcggtgcg cggggatttt gccgtttccg ccaccaagct cgccaaccac
    23581 ctgaaagcgg tgcacctcga gatggcgtcg gaggatctcc tgcgatccct gggcctctac
    23641 ggcggtttcc tgtcccccgt ggccctgtcg ggggttcgcc tggtcctgga tacctccgtg
    23701 gacgaccggt cctcctacgt ggccgggggc aacgaaccgg acgttcattg gaaaaacgtg
    23761 gtgtacggga gggatttccc ccgggtcgac cgggtggacg tgaccgaggt ccgggaaggg
    23821 gacggttgcg cccagtgcaa aacgggaatc ctcgaaattc gccggggcat cgaactgggc
    23881 cataccttca agctgggcac caagtatacc gcggaaaact ccatggacgt gacctacctg
    23941 aactcggcgg gagaaccaca gagggtggtc atggggtgct acgggattgg agtcgagcgg
    24001 ttggtggccg ccgtcatcga gcagcaccac gatggaggcg gcatgattct gcccccgacc
    24061 atcgctcctt tccaggtcat gctgacttcc ctgggtggga agcccgatgt ggtgtccacg
    24121 gccgaggaaa tctaccggag cattcaatcc cgcttcgaag tcctctacga cgaccgggac
    24181 gaatctcccg gggtgaagct caaggatgcg gaccttctgg gaattcccgt ccgggtggtg
    24241 gtcagcggga aatggctccg cagcggggaa gtggaaatca aggtccgcaa gacgggcgaa
    24301 gtcttcccct gtgcccggaa ggatctcgag gagaccctcc atcgggtgat ccgggacctt
    24361 cagccttccc tggacggcct gccgtacctt cccgagtagg cacctccgcg gatcgaaact
    24421 cgaccacgaa aacctcccgg gtggaggccg tcaccttcac ccggtcgtcc aaccgatagc
    24481 ccatcaccca acagatcttt tcctcgtcgc aaagcagggg gatgcgatcc cgctctgccc
    24541 ggggaatctt ccggtccgta aagaagtcct ggagtttccg ggacccgccg aggcccacgg
    24601 gccggaagcg atcgcccttt ctccggaagc gaaccgagag actcccgcag acggcgctgg
    24661 cgtccaggca cgctcgatgg gaagacttct ccccttgccg ggcctcttcc ggtccatgcc
    24721 gtgacaggga aatctcgaat cccgagaagg ggtaggtccc cggcccggat agggtgatcc
    24781 gggcgtcctc gttctccgga gcgattggag cctcccgggt ccccaggacg aggaattccc
    24841 gggagcgggt cagccgaagc tttcccggca ggtgcatgga ctttccctcg gacgactcgt
    24901 ccacccaccg ggacatggca tcccagtgac gggaatacag tccgagggtg tttccccgga
    24961 gcttttccac ggcccacctc aggacccggg tccgcaggac cgggtgcagc cctcgaaggg
    25021 cttcgacgtt caggtggacc cgggaggggg tctcctcccc gaagaccgta ggccggcact
    25081 cgaggacccg ttccttccaa taggcctctt ccctgcgcac cagttccgca tgacggcaga
    25141 gggtctcgac gacccgggaa tgaaagctcc ggtgcaggac gggaaagacc tcgaggcgca
    25201 ggacgtttct ctggcaaaag ggttgcaggt tggacgggtc ctcgatgtag gaaatttgcc
    25261 ggtggtgcag gtagtccagg acctgccccc gcggggcatc caggaggggg cgaatgatcc
    25321 cgtgggcgtc cttgggaggc atcccgccca ggcccgaagg gccgctgccc cggatgagcc
    25381 tcaggaggac ttcctcggcc tggtcgtcgg cgttgtgacc cagggcgatc ttggtacccc
    25441 ccaggtcctt tcgtgattcc tcgaagaagc ggtgacggca tacccgggcc gccatctcga
    25501 gggacatgcg gtgttctcgg gcgtattgcg gcacgtcccg ggtcccggcc cggaagggca
    25561 gtcccagttc cccggcggcg gatcgaaccc gctcctgctc ctcgatggag gcgtttcccc
    25621 ggatttggtg gttgaaatgg agcacggtga gtcgagggtt tcccagctcc ccccggatgg
    25681 atgccaggac gtgaagcatc gccgtggaat cggggccgcc cgacactgcc gccagcacgt
    25741 ggtcccgggc tgagatgagc tcgtggcggg aaacggcttc ccctacaagg atctccaggg
    25801 ggtggggagg actcatgagg gtttgggatc ggactggggc cggagcggcg gcccggaagt
    25861 ctcctgcctg cctcccgggg gagtgtcggc cggaagaagg ggaagcagct tcttcccgtg
    25921 gctgatcagt tttccctcgt tgttgaggac cgtgttgtcc tcgaggatga tcaggccgaa
    25981 ccgctccgcc atctcgctcc ggtagcggta ttccatggat ttcttgcgga gctgaaaatt
    26041 caggaagatg aggacgaggg cgaaaaccga aaggatcgcc acgagaacgg aggcggaaat
    26101 caagacgatc ttgacggatt tttctcccac gttgctgagg aaggccaaca gaacgtccag
    26161 cttgtagaag aagcccacgg ccactcccaa gagtaggagc aaccccagaa acgggagccc
    26221 ctgcagccag tgcaccagct gtcggagctt gtacacccgc ccggcctgtt ccccgttgcc
    26281 ttcggcggga aggtccggga aaaccgcctc cccgagggtc tcggtctccc cgaagtactt
    26341 gttgaaaccg accacaatca tgcccagcag aaaggtgatg gccacgggtg ctaagaaggc
    26401 caccaggaag tagaccttcc agggaaaggg ggcggagacg gggccggagg ggtcggatcc
    26461 ccgatcgctc agttggtaaa accagcaagt ggcgaagatg aggatctcga tccatccgag
    26521 aaagaagagg tacttgtgaa acagctcttt gcgttcgaaa tcgtcgaaga gggcggctac
    26581 accgcgcagt cgcttggggg tggagtcggg cgaggtcttc atggctgtat ccccggggtt
    26641 cgcctgaatt tgttttgaca tctatccaga aatacttgat atccagtccc cggcaggata
    26701 acaaatggac ccaggagggt caactcggat ttggcgggcc gtaggcgaca attcctatct
    26761 ttccaggagg atccatatga ccgaccaggt ggtattccaa acggatttga agggactcaa
    26821 acttctttct cggggaaaag tgagggacat ttacgacctg ggagacacct tgctcatcgt
    26881 ggccacggac cggatttcgg ccttcgatgt ggtgatgccc actcccattc cggacaaggg
    26941 ccgaattctc acccagcttt cggcgttctg gctcgagttc ttgggaagaa tcgtccccca
    27001 gcacctcatc agcacccggg tccgcgagtt cccttccgtt tgcgatcctc atgccgccgt
    27061 tcttgcgggc cggtccatgt gggtgcgcaa ggccgatgtc ctgcccgtgg aatgcatcgt
    27121 tcgaggctac ctggtggggt cgggatggaa agactatgag aaaacgggga cggtctgcgg
    27181 cctcagcctg cccccggggc tacttgaggc ccaaaagctt cccgaaaccc ttttcacccc
    27241 ttccaccaaa gcggcccacg gcgcccacga cgaaaacatc tccttcgagg aaatgggccg
    27301 aatggtgggc agggacgttg cccgacaggt caaggacgtg agcatcgccc tctacgaaac
    27361 cgcggcctcc tatgccgcgg aaaaggggat cctcctggcg gacaccaagt tcgaattcgg
    27421 cctcaaggac ggggccctga tgctggtgga cgaggtgctc accccggatt cgagccgatt
    27481 ctggcctgcg gaccgctacc gggtggggac cagcccggaa agttttgaca agcagtacgt
    27541 gagggattac ctggtgcagt cgggatggaa accctcggat ccggcccccc agttgccccc
    27601 cgaggtggtg gccaacaccc gctcccggta cctggaagcc ctgaaacggc tgacaggacg
    27661 aagcctggaa accctgtgac ggatcgaacc ctttctcccg tgcgcctcga ggatatggcg
    27721 tgggaagaca ccacctttgc cgtgccctgc tattcgtccc gggacctctt gctggaatcc
    27781 ctggggcgaa acgggattct ccatcccccc tgggtgtggg atgagcccgg gggaaagtat
    27841 gtcattgtgg acgggttcac gcgcctccgg tgggcacggg agtccggggc ggaaagcctg
    27901 ccctgtaccg tcttcggccg ccggactccc cgggagcggc ttgtttgcct ccgaatcgag
    27961 gaaaagctcc tgggttctcc gctcaacccg gccgaaaagg cgcggctggt agccctcctg
    28021 ggtgaaatct ccccggtaga aaaggatttt ccccggctgc tttccgccct ggggatttcc
    28081 ggtcgccggg agatcgtgga ggcatggcgt cggctggatt ccgccggggc ggaactgctc
    28141 gaggcggcgg catcgggggg aatttgcgaa cgggcggccc tcgagcttgc ccggtgggac
    28201 cccggttcgg cccgggaagt gctgggatgg tttcgtgttt tgcgatgcag cgccagtatc
    28261 caggtggagg tcctcgatcg gatcggggag atcgccgccc gggagaagcg ggagaagctc
    28321 gatctgctga aggacccggc ctggggctcc atccttggga gctcccactg gaaccaccgg
    28381 gaaaagaccc gggaacttcg caacttgctg gcccgccgcc gctttccccg gctccatgcc
    28441 cgcatggatc gcttccggcg ggacttggcc gggctttccc ttcccgagag tcttcagctg
    28501 gtcccgcccc gggattttga aggagaccgt tgggagctgc gcctgggctt ttccacggag
    28561 tcggacctgg cccggggggc gaaggcggcc caagggctcg ccgagtccgg aaagctcgat
    28621 gaggtccttc atcccgatcg gtttgccggt tccccgtgaa gcatcccatg gacggtttgg
    28681 tgaagccccc gagaaaggtc ctcgtggaag ccggcctgga agacggcgaa ctggtgcgtt
    28741 gcctgagaag caccctgccc cgtgccgact tccgcgtgct ggaaaagatt tccgatcgtt
    28801 cgtccttcga ccccgaagtc ctcgaggtgg tggacttccg ggggcggttc ctcaagtcct
    28861 gccccgggac ccggcactat cactgctgcg ggtaccaaat cctccatttt ggaatccagt
    28921 gcactctggg ctgttcctat tgcatcctcc aggcctacct caaccagccc aacctccaaa
    28981 tctttggaaa ctgggaggct ctgttcacgg aaatcgagga aaagctccgg gctcagcctt
    29041 cccggctcca taggatcggc acgggggaat tcaccgattc ccttctgctg gacccctgga
    29101 cgggcttctc ccgcaggttg gtgtcctttt tcgcccggca gcccaacgcg gtcctcgagc
    29161 tcaagaccaa gaccaaccac gtggggaacc tgcgggggct ggatcaccgg cgccgcacca
    29221 tcgcggcctg gtctctgaat gccccgtata tccgaggccg agaggaaacc gcggcggcgt
    29281 ccctgcgggc aaggctcgag gccgcccgag cctgcgcccg ctgggggtat cccctggcct
    29341 ttcacttcga tcccctggtg gagtaccctc ggtggaagga tgggtacgcc cggatcctgg
    29401 accggctttt ggattccgcg gaccccggcc aaatcgtgtg gatcagcctg ggatgttttc
    29461 gtttcatgcc ttcgctgcga tccgccgtcc tggcccgcca cccccggtcc ggggttctgc
    29521 tgggggagtt cgtccggggc ctggacggca agatgcgcta cttccgggat atccgggtgg
    29581 aaatgtactc gttcatggtg gagcgcatcc gaaaggcgga cccgggcctt tgcgtgtacc
    29641 tgtgcatgga gggcgaggac atctggcgcg aggtcttcgg gttccagccg ggggagaagg
    29701 gaggcctgcc cgccatgctg gaccgggcgg cccgggaacg aacggggatc gaggacggag
    29761 cgtcccaaaa gaatccctag gttttctctc gaaattcaga tattaggttt ggacgagctt
    29821 ggaatcggcg gatcgaattc ccggtgctcg cgaacacgca tcctggacgg aaagatgagc
    29881 tcgtagaacg gaggaatcca tggattttca gtttaccgac gaacagcgaa tgatgaagga
    29941 aaccgtgtac aagtgggcct tgaaggagct ggggcccctc caggaaaaga ttgacgaaga
    30001 ggactggttt ccccccgatt ttttcaaaaa gtgtgcggag ctggggatct tggggatcac
    30061 catctccgaa gagtacggcg gtctcgacgg ggacatcctc atgcaaaccc tggcggtgga
    30121 agagatgagc cggatttgcc ccgccctggc catgacctac ggaacccact cgaacctttg
    30181 tgccaacaac attcatcgca atgcaagccc cgccctgaag aagcgttacc tgccgcccct
    30241 ggtggcgggg gaaaagatcg gggccctggc cctcacggaa cccaacgccg ggtccgatgc
    30301 catggggatt cgaacccggg cggtgaaaaa gggggaccgt ttcattctca acgggagcaa
    30361 gatgttcatc accaacggac cggtggcgga catcctccta gtgtacgcca agaccgtgcc
    30421 ggaaaagggc gccaagggca tcagcgcctt catcgtggaa aaggacttcc ccggcttctt
    30481 cgtttctcgg agcttgaagc aatgcggcat gcggggatcc cccacggggg agttggtgtt
    30541 cgacaactgc gaggtcccgg aggaaaacct cgtgggggag ctcaacatgg gagttcatgt
    30601 gatgaccagc ggcctggaca tcgagaggat cgtcctggcc gggggatcca tcggcatggc
    30661 ccagcaggcg ttggattact ccatccgata cgccgtggaa cgggagcagt tcggccagcc
    30721 catcgccaat ttccagatga ttcagcagaa actggcggac atgtttgccc gtaccgaagc
    30781 cgcccggctt ctgctctatc gggcggcgac gatggcccag gagtctcacc gggggggcaa
    30841 ggggaccgag ctgaccaagc ttgcggcagc ggcgattctg ctggcttcgg agacggccac
    30901 gtgggtgtgc ggacagggag tgcagatcca cggaggctac ggctactgcc tcgagtttcc
    30961 cgtccagaag ctctggcgcg atgccaagct ctacgaaatc ggggcgggaa ccagcgaaat
    31021 ccggcgtatg atcatcgctc gggagctcac ccgggaggaa tttgcccggc gaaggcagcg
    31081 atagcgctta cccggccgtt cttccggcca ctacacgaga gagtccaaag atggaatacg
    31141 gattgaggcg gtacgaggcg gcccaaaggc tcttctggat tctgtttttt gtcctgtgtc
    31201 tcggcgtggc gtacctgttt gcggaggcgg ccgcgaattc gcccaacggt tccgccgccg
    31261 ctttgcccac gtttcagccg gactctctca agtccctgag cttctggctc cagctgcggt
    31321 ggatacgagg agagcccttc gggaaaatcc tggtctacct tttgtacgtg gccttaatct
    31381 ttgtgggagc ccggttctgc tggctgctcg tccagtatgc cggaaagttc tacgtcaagg
    31441 gcctgctggc atcccacgtt cgcaaaccgg cggcctcccc caagccgtcc ttggaacagc
    31501 tttccgccaa gcccgagaag ctctttcccg tggatatcct gctcaaaaag tccaatcccc
    31561 tcttcctgcg cctgcttttt catcccatga cccgcctgag gctgctgctg gcaaagaacc
    31621 aggggaccct ttcctcggaa gatctcatgg aaagggaaag gcgcatggtg gaaacggact
    31681 ggcaggtcct ctacgggtcc tggactccct ttcggtggct tctctggctg cttcccctct
    31741 ttgccctgct gcaatcttcc tggctgctct accaccaggt gcagcccgcc gttacgggac
    31801 aaaaggagat ccacgacgtc ctgggcccgg ttttcgccag tttcatcccc ctggtgcaaa
    31861 tcatcctgat caacgtgctc ctgagcctgg gatcggggct tctcaagcgg atggaaggct
    31921 tctatctctc gggggtggac acccttttct acgaccggtt tctgtcccgc ctgcccttcc
    31981 agagcagcga caccttgatc ttgctggagg cgttgaaccg gcatttccaa gaactgcatt
    32041 tcgtgctgcg tcggctggaa aagagcctgg gtgacggaaa gtaaccatgg aggaaggagg
    32101 gtggagggaa aaaaatcccg ggacgacccc ggcatcggac agtggatccg caaggtcctg
    32161 gaaaagcacc cggcgttcct ggccaacccc gtgggggatt ggaacgagat cgtgggagag
    32221 caggtcgccc ggtacaccca gcccgtgtcc ctcaaggaca aggtcctcac cgtagccgcc
    32281 tacgattccg tctggaagta ccacctggaa gtcctcaaag aacccatcct ggcgaaaatc
    32341 aaccggaaac gccccattcc cctggtggaa aagatcctgg tgcgggtggg ggaggttccc
    32401 gaaccccagc cggtcttgaa ccgaaaccac cgcctgcttt cccagatcaa gggaaaacgc
    32461 tcccgggccg ggacggcgaa aaaatccccc ttgcggtccc tcacccccga ggagaaggaa
    32521 tttctaaaac ggcttcccga caacgatctg cgaaagatcg gggcccggct cctgcgcaag
    32581 acccccctcg aggacgaatg aattcttccc ggcacaaacc ctcgggcctc gtgcagatcc
    32641 ttccccttcc cgtgggcctt cttggatccg gccttcttcg acgaaccttg cttcgccttt
    32701 cgccccaacc ggttgaaaag agggagtccc ggccgggaag cccttggccc gggtccggca
    32761 cgccctttac cgggtcgtcc ttcattccgt cggtggggat tcggggggtg cggcgtccaa
    32821 agccccgtcc ggggaatccc atcgcgagga tgcctccggg gattgggccg acaggaaatc
    32881 gaaaatcgcc tttgccacgg cggggggaat tcccgggacc tgttccagtt cttcgaggga
    32941 agcctcccgc agggaatcca tgcccccgaa atgatccagg aggattcggc gccgtttgac
    33001 cccgatgccg ggaacggcgt ccagggccga caggagaagc tcccggcgat ggagtgcctg
    33061 gtagtgggtg acggcaaagc ggtgcgcttc gtccctcagc cgctggagca gatgcagaat
    33121 gtcgggaaag cgggcgagga acaggggatt gcgacggccg ggaacataaa tcttttcgta
    33181 aagcccccgg cccttttctc ctcggtcggg cccccgctcc ttggcgatgg aaatgacggg
    33241 aagccgatcg gccatgcccg tctccgaaag cacccggacg atgcggttca actggccctt
    33301 gccgccgtcg agcatgagca ggtccagggt gtccgccagt tcccgctcac gggtcacgag
    33361 ccgctcgatc acctcggcca tcatggcggg atcgtcgggt tcctccttgg atgcgattcg
    33421 atagcggcgg taggattcct tgtgggggcg gccgtcggag aagaccgcca gggctcctac
    33481 ggcatggcgg ccctggaggt tggagatgtc cacgcagccg atgcgaaggg ggggcttggg
    33541 gagccgcagg atcctctgca atccctcgag gaggctgcga tcccgttttt ccgattttcg
    33601 gcgactttgg agcctttccc gggcgttctt ttcggccaga tcgaggagct gcttcccgcg
    33661 gccccgccgg gccggccgga tgtgaacccg ttttcctttc aagtcccgga gccactcttc
    33721 caggacctcg atgccctcga aaggtccggg aaccaggatt tcatcgggga tgaagcggtc
    33781 ttccccgtag aactgctgga tgaaggaggc caggagctct tcggtttccc cgtgggcgtc
    33841 cttgaggtcg aaatgcctct ggcccgccgc aaccccctgg cgcacaaaca ggacggccat
    33901 ctcgatcccg tccgggtccc ggtggatccc caccacgtcc tggtccagga agcggtcgga
    33961 tacgattacc tgcttttcga gggtggtccg gacggcatga agtcgatccc ggtagacggc
    34021 ggcccgctcg aactccaggg actgggacgc ctcctccatg ctcctttgga gctgtctctg
    34081 gagggcgtcc gtcttcccct ggagaaagag aaccacctcc cggaccatcc tcccgtattc
    34141 ctccgggtcg atgttgccca cgcagggggc cgggcatcgg cccatggaat gattcaggca
    34201 ggcccgttcg cgccgggaaa gacgcttgga cttgcagagg cgaagaggga aaagctggtg
    34261 gagaaaccgc accgtctccc gcacggcatg ggccgaggga taggggccga aatagagggc
    34321 accgtccttt tggatccttc gaacgatttc caggcgggga aaagggtccc ggagatccag
    34381 gcgcagggcg gggtagttct tgtcgtcccg gaggaccacg ttgtaccggg gccgatactt
    34441 cttgatgtag ccggcttcga ggagcagggc ttccttttcg gtggcggtga cggcaaattc
    34501 cagggaggcg gtcttggaga gcaggacccg ggtcttgatg ttttccgagg gaggactccg
    34561 aaaatagctg ctcaccctct tcttgaggtc ccgggctttc ccggcgtaga ggatcgttcc
    34621 ggcgcagtct ttgaaaagat agacccccgg cacgtgaggc agggcctgga cggcctccgc
    34681 caggggttgt tcccggggtg tggtgagaag aaagtctggt tccgtcatgc cgtgtcgtgc
    34741 gaccgcgccg gatttcgtgc ccgggcatgg tttcccatgc cgcttccgcg gtatccatat
    34801 cacattcccg gggaattcca aagggtgccg gttcgggagt tcggccgttt tgtcaagcat
    34861 tgcacccggt tgtgctttca aaagggtgga cagacccggc aagatctgtt acaagcagtc
    34921 ccgtcgggac gtggctcagc ctggtagagc gctgcgttcg ggacgcagag gccgctggtt
    34981 cgaatccagt cgtcccgacc aaaaaaccaa accttcttgc acggcaagtg cattcccaaa
    35041 gcaagatgtc cgtgtcacga attgtgatgc acggcccttg agattccgtc ggcacagagt
    35101 gggtccttgt gaggcacgga cgttgccaac cgtccacccg ggcactccct gtgccgacgt
    35161 gttttgtggg ggggacgatg agggccggga atctcgaact cggatttgtc atgaatgagc
    35221 acggcttcga gggctcctct ccgtcggctc gatttctcct tgaaggcatc gagtgacgag
    35281 tgactggccg agcgccccgc cggacaggcg gccggggggg ccgcactttg ccaccggctc
    35341 aatagtcccg gccgaaaagc ccaccgggcc tgccatagca gggtgttgaa aaagcaacaa
    35401 atatgcgtgc attattttca ggtttgatgt agaatcatct cattgaaatc gtttagaaaa
    35461 gatggagatg attcatgagg acccccgatc accggcagca aaaaatgttc ttttacgttt
    35521 ccgtggagga gcgtgtcccg aagaaccatt ctctgcgacc tgtcaagg
//
LOCUS       DAOOVC010000026        34504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_827_length_34504_cov_6.935209, whole genome shotgun sequence.
ACCESSION   DAOOVC010000026 DAOOVC010000000
VERSION     DAOOVC010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 34504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34504
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_827_length_34504_cov_6.935209"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLM79_05480"
     CDS             complement(<1..757)
                     /locus_tag="PLM79_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HOV85791.1"
                     /translation="MPKEFKLPDLGEGIHEGEIVEVLVAVGDRVREGQTVLVVETDKA
                     TTEIPSPVTGTVHEIRVKPGDTVQVGSVLITFLEEGDTKGPTFGVQVGEKTGQTVVLP
                     GEGGTEAAAVGSPAKDLSAAPPTSEGPVPASPSTRRLARELGVDLRRVTPTGPAGQVT
                     SEDVRAYAESVKRAPEAPPKPAPPTGAPPQVPPEPEMAAPSPEAPFPATVERMPLRSV
                     RRTTSRQMARSWSQIPHVSHEETADITELDALRR"
     gene            complement(805..1785)
                     /locus_tag="PLM79_05485"
     CDS             complement(805..1785)
                     /locus_tag="PLM79_05485"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOV85792.1"
                     /translation="MAKLNMVQAINLALQQEMEKDDRVIVLGEDVGVDGGVFRVTEGL
                     IEKFGPERVVDTPLAESAIAGMSIGMAVYGLRPVCEMQFSGFSYFAMHQMESHAARMR
                     WRSMGRFHVPMVLRCPYGGGVRALEHHSESREAYWAHTPGLKMVVPSGPRNARALLVS
                     AIRDPDPVVFYEPKAVYRAFREEVPEEEETLPIGKSRLVREGGHVTLISYGASLRPTL
                     EAASDLHDKEGIEAEVIDLLTLSPLDDELFAASARKTGRVVVVHEAPRSFGPGAEVVA
                     RLVEKSFLYLEAPVRRVTGYDLVIPLFQREQQYLPNAPRIVRAVHQVLDS"
     gene            complement(2006..3109)
                     /gene="pdhA"
                     /locus_tag="PLM79_05490"
     CDS             complement(2006..3109)
                     /gene="pdhA"
                     /locus_tag="PLM79_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HOV85793.1"
                     /translation="MPRKEIDLPHRVETLSILDENGVLDKSLEPDLPDDLLLRLYRAM
                     ILGRRFDERMLILQRQGRIGTFAPIKGQEASQLGAVAALKPEDWMVPSFREGAAQVWR
                     GQDMESLLLLFGGYNEGGVIPPGVNNLPISIPVGSHTLHAVGLAYAARYRKQEQVVMT
                     FFGDGATSEGDFHEAMNFAAVFRAPVVFVCQNNHWAISIPRSRQSHSSTLAQKALAYD
                     MPGIMVDGNDVLAVYTAAREAVDRARAGGGPTFIECVTYRMSVHTTADDPKRYRKDEE
                     VEEWAKRDPILRFQKYLIEKGLLTEETVQSIEEQVQNDIKGVEARWEKRIQGPLDPLK
                     MFDHAYAELPPSVLEQKAELAAELREQQGKAGS"
     gene            complement(3367..4005)
                     /locus_tag="PLM79_05495"
     CDS             complement(3367..4005)
                     /locus_tag="PLM79_05495"
                     /inference="COORDINATES: protein motif:HMM:NF008320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HOV85794.1"
                     /translation="MFVWTEYIKIFTALLVIVNPIGVLPIFVSLTGHQSAKERRRTAL
                     VSSVTMGVVLMAACLLGDSLLRFFGISVASFRVGGGILILLIAVAMFHAQPSASKQTP
                     EEAAEAGEKADVAVVPLAVPLLSGPGAISTVIIYAHQLPDWKHAGLLLAVCLLVALTV
                     WLTLRLAIPIGNALGRTGINIVTRLMGLILAAIAVEVLVGGVTVLLPGLVQR"
     gene            4460..4822
                     /locus_tag="PLM79_05500"
     CDS             4460..4822
                     /locus_tag="PLM79_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOV85795.1"
                     /translation="MRRRGHSHGNGFCGIQGTPFPYEEHRKAAQQAVEIAREKLNSER
                     VDLLIPDEINNTLHLRLVDLEQVLDLIGAKPPRMHLVLTGRDAHPRAIEPADTVSEVV
                     EIKHAYGKGIEPQPGIDS"
     gene            complement(5008..6192)
                     /locus_tag="PLM79_05505"
     CDS             complement(5008..6192)
                     /locus_tag="PLM79_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV85796.1"
                     /translation="MEDRKKSTRAVHLGGVKSSVYGEVVPAVFQTSTFAFPSAEEGAA
                     RFAGERPGFIYTRLANPTVRVLEEAVASLEGGSGGVAASTGMGALTTTFLALLEQGAH
                     VVSTDSIYGATHLFLESDLSRFGVRTSFVDTSNPEILRDALGPETRLVFIETPTNPTM
                     KITDLEAAARMAHEHGALLVVDNTFASPYLQRPLRWGADIVVHSLTKFLNGHSDVLGG
                     MVTAKDPQLLSRLRKASSTYGSTMDPHQAWLILRGLRTLALRMERAQENALRLAQYLA
                     GHPKVTWVRYPGLPDHPQHAVARKQMEGFGSMICFGVKNGYEGGVTLMDRVRLITLAV
                     SLGSVESLIEHPASMTHAAVPWEERQQAGILDELVRLSVGCEDFEDLRRDLDQALSQV
                     GK"
     gene            6776..7675
                     /locus_tag="PLM79_05510"
     CDS             6776..7675
                     /locus_tag="PLM79_05510"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOV85797.1"
                     /translation="MPIHSRSFAVIMGTVVLLFVAGCAPVKQLRVATVALTLRDVADA
                     AGKQSSPRVIREGTPAYLMLLDGLLEAEPRSRELLLAACRAYVSYASSLVGQGNPEEV
                     SVLFFRGRDFGFRALSESLQTNFKEASSGTLDGFAAVLAKCGKKDVPALFWTTNAWAN
                     WIALNTSKVEALGDLPMLEAAMKRTLELDETYFYGGPHVIRGIYLASKPAVLGGNPAQ
                     AKVHFDRARELSGDKNLVLKVYLAEYYARSVGDRELYRRSLEEVLAAPLEEGSDMTLS
                     NVMAKEKARWLLDKTEEYFEPER"
     gene            7659..8738
                     /gene="dctP"
                     /locus_tag="PLM79_05515"
     CDS             7659..8738
                     /gene="dctP"
                     /locus_tag="PLM79_05515"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOV85798.1"
                     /translation="MSRNGRSIVRWFKGKALLPCLFSVLTGCFAVMGSVWPSPALGAK
                     PEHEIKLATMAPENSSLMKIFHEMNEELLKETGGKVGFKMYAGFVLGDEEDVLRKLRV
                     GMVHAAAFTAAALTDLSPDLRVFQVPFLFADYKEVDFVLDRMESDLKKGFEKKGFYVL
                     GWPELGFLYFMSTTPISSLEDLKGKRVWAKANAPMLEGLIRKTGVSAVAINAPDVLVA
                     LQTNLLDVVYNSPYYAVVTQWYTQFKYVTDLPLSYIGGALILDKKVLDKIPAPLRETL
                     RKVCDKHLRRLTERTRKDNAESLQVILKRGVTQITPKAAEVEEFKRVGDEAVMALDPK
                     YFPKDTFQKVKVWLSQYRTASPNPK"
     gene            8696..9241
                     /locus_tag="PLM79_05520"
     CDS             8696..9241
                     /locus_tag="PLM79_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOV85799.1"
                     /translation="MAFPVPDGFPQSQMTVPPLNPCRRALHHLHDFEKWVFVGLTLFL
                     AGFALLQIILRNFFSTGFIWGDTLQRHLVLWISLLGAARATLEDKHIQVLLLPVLLPP
                     RAARFLPYLSDGFSLAVSAILLYAAWIFVGNERAAGDLAFSGIPYWWLEFIFPVSFAM
                     MTARFGFRLSGRLFGAKGGNP"
     gene            9238..10503
                     /locus_tag="PLM79_05525"
     CDS             9238..10503
                     /locus_tag="PLM79_05525"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOV85800.1"
                     /translation="MTLLAVLGLLFLTLSGVPLFVVISAAALLCFRYLGTDLSVVIIE
                     MYRLAANPMIVALFLFALAGYILAESKASRRLVRLSTALLGGAPGGLAVVALLSCALF
                     TALTGASGVTIVALGGILFPALTADHYRENFSLGLLTTCGSLGLLFPPSLPLIVYGMV
                     AEANISHLFLAGLLPGLFMMALLGAYSMYQGTRFQAPGSRFRFSLKELVGALNESKWE
                     ALLPVVVLGGIFSGYLVMSEAAAVTVFYVFLVEVVIHRELRIRDLSSVIVKTVMMVGG
                     IIVILGASLAFNSFLIDQQVPDRIFEFIQSRIESKIGFLLLLNGFLLIVGSLLDVFSA
                     LIIVVPLIVPVAANYGVDLIHLGIIFLTNLQIGYSTPPIGMSLFIASLRFEKSILQLY
                     VASLPFLCILLAVLAVITFVPSLSLWLVP"
     gene            10532..11227
                     /locus_tag="PLM79_05530"
     CDS             10532..11227
                     /locus_tag="PLM79_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HOV85801.1"
                     /translation="MRAHYLQHVPFEGLGSIESWLDSNGYELTHTRFFESVDFPDPEA
                     IDLLVVMGGPMSVNDEDQFPWLVLEKRFVREVIESGKSVLGICLGAQMIAHAMGAKVF
                     PNPVKEIGWFPVRGVASVDASVFRFPPSEIVFHWHGETFDLPPGAVRLAKSEGCENQA
                     FQLGKRVIGLQFHLETTPKSAREIVSHCRHELVPSRYVQTEEEILSASPSRYQSINDL
                     MGGVLSFLQRGDG"
     gene            11850..12242
                     /locus_tag="PLM79_05535"
     CDS             11850..12242
                     /locus_tag="PLM79_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85802.1"
                     /translation="MVKKGIDDDHLMEEFDEDGGLEFDQTDELDFTGEDDLLMEEGDE
                     EDLIPSVLYKGVSRPKTDEDWRQLLLDASRDGVPEYKMSDTYKEGDLIIHPSFGLGVV
                     SKLISPRKAEVIFENSKRLMAMGVTPPQ"
     gene            complement(12270..13115)
                     /locus_tag="PLM79_05540"
     CDS             complement(12270..13115)
                     /locus_tag="PLM79_05540"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOV85803.1"
                     /translation="MSVEPGLIREMEEVARKATLKAGKLIAGRLGKVPLSSVQNKGPS
                     DYVTEVDKEAEALILDSVSRSFPGHRLVSEEANLARIGNGIQWIIDPLDGTTNFIHGF
                     PFVAVSVAVYEGLQPLLGFVLDPLREELFHAVRGGGAFLNGSPLRVRERFTVEQALIA
                     TGFPFRMKRILDPYLAAFSSILRQVSGIRRAGSAALDLAYVAAGRVDGFWEAGLAPWD
                     VAAGSLLVLEAGGRVDDFWGEGAYLLNGHIVAGTPLVHPFLRERIGEHLAPALKTGEQ
                     GGKTV"
     gene            13291..14175
                     /locus_tag="PLM79_05545"
     CDS             13291..14175
                     /locus_tag="PLM79_05545"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV85804.1"
                     /translation="MKIDVHTHIFPPEICRDRGRFFSSQPAFRLLYDSPKAPLATAER
                     LLEVLDEDEVDRAVVFGFPWQGRERVLRHNDYVLEAAARYPRRLIPFACVHPLDPEAP
                     AEADRCCAQGAAGLGELGLYEPCDPAAALAAYDGIFRVARSYGKPLLVHANEPVGHAY
                     PGKAPQGLDFYYRLAQRAEGIPLILAHWGGGLCFYEMMKKEVAETLSHVFYDTAASPF
                     LYRASVYRTVLDIVGKERVLLGSDGPLLRPRRYFHEMQGVLSPEEMRAVCGENAGRLL
                     SGARDDGFEGPSLTDASP"
     gene            14442..14981
                     /locus_tag="PLM79_05550"
     CDS             14442..14981
                     /locus_tag="PLM79_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85805.1"
                     /translation="MERVPFGEEEIAVLRESVEISEELISDFFKISTSEWKRYRYDIQ
                     SLKDLADGEITDHAFAQIRRYQRSPHLRLRGSEPGDYFKICLQDHVIRQAIVRDAHVR
                     LFPLTAYIATHELIHVVRFARFLQSFDSTPAEQLAEEKRVHVLTEQLLAHCKIPGLAE
                     VLGAFRECYTMETFHKAAD"
     gene            15119..15409
                     /gene="gatC"
                     /locus_tag="PLM79_05555"
     CDS             15119..15409
                     /gene="gatC"
                     /locus_tag="PLM79_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOV85806.1"
                     /translation="MKEKITREEVRHVADLARLELSEQEESRMTEQMNQILGYMDQLN
                     ELNTEGVSPTTHAIELRNVFREDEVRESLPREQALANAPQSDGVNFLVPKVI"
     gene            15434..16897
                     /gene="gatA"
                     /locus_tag="PLM79_05560"
     CDS             15434..16897
                     /gene="gatA"
                     /locus_tag="PLM79_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOV85807.1"
                     /translation="MDLTALTIHELRRRLVRREVSVTEVVRRYLERIEALDPRVNSYV
                     KVMADEALEEAARYDRGEKDLEGPPLAGVPLSVKDVLCTRGIRTSCGSKILENYLPPY
                     DATVIEKLKASGAVLLGKTNMDEFAMGSSTESSAFGPTRNPWDLDRVPGGSSGGSAAA
                     VAADLCAGSLGTDTGGSIRQPGSFCGVVGLKPTYGRVSRFGLVAFASSLDQIGPITKD
                     VRDAALLLHVVAGYDRRDSTSVDLPVPDYPECIREPVDGLRLGIPREYFVEGMHPDVL
                     SRVRQAIETCRELGGEVVEVSLPHTAYGVAAYYIIAPAEASSNLARYDGVKYGTRASD
                     ARELAAMYRETRSRGFGPEVKRRIMLGTYVLSAGYYDAYYRKASQVRTLIRQDFLDAF
                     QTCDALLTPVVPTPAFRVGEKTGDPLQMYLSDVFTLPASLAGIPGISVPCGFTSEGLP
                     VGLQILGPHFREDLVLRIARHFEEAAGFSRRKPDLCA"
     gene            complement(16917..18197)
                     /gene="glgC"
                     /locus_tag="PLM79_05565"
     CDS             complement(16917..18197)
                     /gene="glgC"
                     /locus_tag="PLM79_05565"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOV85808.1"
                     /translation="MKDVITVIMAGGRGERLMPLTRDRSKPAVPFGGVYRLIDLPLSN
                     CINSQLYKIVIFPQYKSQSMVDHIEEGWNIFSMDLGHYIRIAAPQQRLGAEWYRGTAD
                     CVRQNLFLIERERPRHLLILSGDHVYKMDYTIFREYHEQQGADVTVALLEVERSTASG
                     YGIAEVDNNFRIVAWEEKPKDPKPIPDDPERSLASMGIYLFKTEVLLELLKKTDYDDF
                     GKDIIPNYLQTLKIIAYPYRRLNRIKDYIRLMDPEGSRKMQLTEQTRDSQYWRDVGTL
                     DAYWNANMDLTGVDPFFNLYGSLWPIRTFQRQYPPAKFVFNQREGKNPRVGTAVDSLV
                     APGCIVSGGTVRSSVLSYNVMVRSWAEVDESVIMDNVEVGRHCRIKKAIIDKHNVIPP
                     HTQIGIDPAEDRKRFVVTPRGIVVVPKGYFQPDS"
     gene            complement(18282..18821)
                     /locus_tag="PLM79_05570"
     CDS             complement(18282..18821)
                     /locus_tag="PLM79_05570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOV85809.1"
                     /translation="MKKNRGRMTSWWILIACAAALPLLWGAHDVKASPDDIRLASWLA
                     QDKKAAPDFELPDLNGSQVRLSAYRGNRPVLLYFWATWCPSCLAAKPEIAKLRDKIGR
                     DSMEILGINVGNGDTLDRLKRYQQGHPVPWPVLFDAAGKVTRSYRVQGIPHFVLVDTE
                     GNMVYWGNSLPADPKKYLK"
     gene            complement(18818..19525)
                     /locus_tag="PLM79_05575"
     CDS             complement(18818..19525)
                     /locus_tag="PLM79_05575"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV85810.1"
                     /translation="MEWLGQFETIAQQYPMVGLLLAFVGGVLASLTPCSYPMLPITVA
                     FVGGKACGSRWRGFTLSVFYVLGLAVVYSALGAFAALSGQLFGALTNSRWTYLLVGNL
                     CLFFGLAMLEAVPLTPPAFLNRLKVRDLPGHDILTSVLLGGASALVVSTCTTPILAVL
                     LTLVAARQSVAWGIGLLFAFSYGLGTLVILVGTFTGLLSSLPRSGVWTRRVQKFFGLI
                     MVLAAEYYFIKTGELWL"
     gene            complement(19549..20310)
                     /gene="recO"
                     /locus_tag="PLM79_05580"
     CDS             complement(19549..20310)
                     /gene="recO"
                     /locus_tag="PLM79_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOV85811.1"
                     /translation="MTTRTTEAMILQTRDYGESDRLVSFLARTGGRLQGIAKGARRSK
                     KRFVHAFEPGSLVEVTYRARKSLAWIEGCKLIEPHLGLRTDVERWGYAALVSEILLEM
                     VPEAEPQDELFELVRQTFGRLTGDKDPVNVVVLFLLRFLDLAGYLPSLEACGVCGKPL
                     SRARRWNWDLHRGTLSCPQHSPFPSEALALDLGTLMLIGRSRTVPLDRLWRLRVAQGR
                     KMSVLRALTGWVQGYIRKDLKSLHLLDQVRSTQDP"
     gene            complement(20584..21537)
                     /locus_tag="PLM79_05585"
     CDS             complement(20584..21537)
                     /locus_tag="PLM79_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOV85812.1"
                     /translation="MKELSEYLKSERLKRGRSLKEMSGEVRVSVGMLQALEEGDFDRI
                     GTTLLIRSFIRAYCSSLGIDPAPLLERYEAEIRAQDKQEEGIRRYGIWMRSLRGKRRV
                     GILVLLILALLLAAAIYGGVWYSERLARLAVLQQTGPDVNPQQELPPDLIQGTLAPAQ
                     AGLHKEVKPSESRTLDPSAGPGVSTGKSGGPAVVSLQSPEAPDGTSAPSPPPTDRAPS
                     DKYPAPATGEAALKRFEVEALELTWVQVRVDDKAEENLHLMPGEKRAWEARKDMKVVI
                     GNPRGVRMMWEGKPLEIKGKPGSVLKFRLPGYAAAKTSKPR"
     gene            complement(21561..22514)
                     /locus_tag="PLM79_05590"
     CDS             complement(21561..22514)
                     /locus_tag="PLM79_05590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOV85813.1"
                     /translation="MKIKEIIFLCLALFLCSGVAFGETVDRIVANVNGEIILYSDIQN
                     RIRSMEKLKKANPGIAPEAISEREVLQQLIREKLAEAEIKRLKITVSDGEVDSAIQNL
                     KAEHHVTDAQFEEALRQEGETLKSFRNTVKKQIEQSRLMDRVLKSKIVITDEQVNAYV
                     RTAPPPTVPTRESLRISVIFLPSGDDGKQAQAAQKQAKEIHNRLKGGSDFGRMAREYS
                     KGPASEEGGDVGFVELGDLAPPIAAAVRKLKKNDFSEPVVTPAGVYIVKLLDVRTERP
                     DPGEMDNKERIRRELYQKEVNRQYENWMKELESRSFIQITL"
     gene            complement(22504..26199)
                     /gene="mfd"
                     /locus_tag="PLM79_05595"
     CDS             complement(22504..26199)
                     /gene="mfd"
                     /locus_tag="PLM79_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOV85814.1"
                     /translation="MATLVIVETSFWSEELFETEDAKTRIMDSPTVVQGLPESSTPIE
                     SFLSQSDSRGILQEIEASGESLSLRGVRPGALAYLLALQLRSGRKPVLLVAATDAEAQ
                     KLADSVTLFLGKNRFSPESPLDRQVWVLPSRSGHKAQWLGKMEATARRIEALYALRAG
                     SSPILVAASARSLLERLPPPEILTQNAEYLVKGDRLDPEALCRRLDEKGYYRTPLVEE
                     YGDYGRRGGILDVYAPLYPYPLRLEFFDDEIESVRLFNPANQRSMGTIEEALLLPASE
                     VVLNAEALERAQKAVYHDVQAERLTPAAGNVWLEKFQEGHQLGAYEAILPVFHEKMVT
                     LLDYLEKRCLVVWSDAAGVRERMDKLYWEVLSEGETRETPHEWKIPPSRLFGDPEDLL
                     TRIESFQQLRVNSLSDGPDTKVFDLGTRGHEELKISIKSHPTRERLLEPLARKFQEWQ
                     SQGIRPSLFCRHAEQARRLTDLLRSYGVDALFSSLPFGEESFDAPAVKVLVGALEKGF
                     YWPRERLAVVSEEEIFEKRGCRRASKPPLGVFLHSFQDLHANDFVVHVDHGVGIYREL
                     VHLSVRGLESDFLLLEYQDGDRLYVPVDKLQRVQKYMGIEGHEPKVDKLGGKSWETAK
                     KKALESAQRIAAELLQLYAVRRVREGFAFSGPDKYLREFEATFAFEETPDQMRAIGDV
                     LEDMSSRRPMDRLVCGDAGYGKTEVALRAAFRAVMDNKQVAMLVPTTVLAEQHYQTFT
                     ERFQGFPIEVAGLSRFKTAAQQKRVLEGLAGGTVDIVIGTHRLLQKDVAFRDLGLVII
                     DEEHRFGVKHKERLKELRVSVDVLALTATPIPRTLHMSLSGIRDLSTIETPPQDRRAI
                     ETYVCAYDEFTIREAIHRELQRRGQVFFVHNHVGSIYQVAARLSQWIPDARIGVAHGQ
                     MKERELEKVMMDFIHRKVDVLVCTTIIESGLDIPAANTILINRADKFGLAQIYQLRGR
                     VGRSSEQAFAYLLIPGEHLISRDAQKRLRALLDFSELGAGFKIALNDLQIRGGGTILG
                     SAQSGHISAVGYELYLELLEKTIREFKGEPVEPETVDPEISLPVSAFLPDSYIADTDQ
                     RLLAYKRLASLTREAQVDDLATEWRDRYGPFPDSTRNLLLLAKCRLLFKRLGVARIEA
                     DEEGFRVQLAPSADSQRFLQVLEQKKCAVSPGTNGRFRVEIWGKHPPHRLARFKRILQ
                     EVEENASATKSIQQLRPHLSLGESHED"
     gene            26283..27725
                     /gene="selA"
                     /locus_tag="PLM79_05600"
     CDS             26283..27725
                     /gene="selA"
                     /locus_tag="PLM79_05600"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOV85815.1"
                     /translation="MTEPTLQSLLRKLPSVDALLHMDSIREALSNHPRKLVIESLRYA
                     IETQRQRILRDPESPDRVDASPRFVARLALEHLQSSSDYTLRRVVNATGVIVHTNLGR
                     SLLPQEAVDRLVTLCRSYNNLEYDLERGQRGSRYVHAEAVLCELTGAEAALVVNNNAG
                     AVFLVLNTLARDREVVVSRGQLVEIGGSFRIPDVMRSSGAALKDVGCTNRTHLRDYEG
                     AINERTAALMKVHTSNYRIIGFTAEVTLEELVTLGRQYRLPVIEDLGSGSFVDLSRFG
                     LLGEPTVQEAVKAGADVVTFSGDKLLGGPQAGIIVGRKDLLDQCRKNPLTRALRVDKM
                     TLAALEATLRLYRDEARAMEAVPTLGMIALSPTVLEERAQGLAALLREADSRGVLEIK
                     VRPCFSQVGGGSLPGQNLAGHGVAVSSTVLSPNRMEMLLRANKPPIIGRIESEQYLMD
                     VRTIQPDEWPIIREAFVRMLERAEGREETS"
     gene            complement(27873..28772)
                     /locus_tag="PLM79_05605"
     CDS             complement(27873..28772)
                     /locus_tag="PLM79_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOV85816.1"
                     /translation="MSHDDYYQILGVSPDASIEDIKKAYRKQALETHPDRNPNDARAE
                     ERFKKISEAYGVLMDARKRAQYDEYRRFGTHRPSGGTSRTGFGYSQEEIFRDFFSSRQ
                     GQDAFWDLQKEFERMGFRFDERFVNRIFFGDKTIFFQSIFWGGPGQTRVFRYTSRGTQ
                     PPGQAARRPSPPPATPAESPGIVSRGISLLAQAGKGLAKSIWKKASEVVSGAVGSGAK
                     PGRRRGSDVTYSLKISPDEAFMGTTVEIEVPHLGRGKKVSVVVPPGVKPGTRLRLKEM
                     GLLHSTRTSGRRGDLYLQLRVVP"
     gene            complement(28964..29773)
                     /locus_tag="PLM79_05610"
     CDS             complement(28964..29773)
                     /locus_tag="PLM79_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85817.1"
                     /translation="MLPADAPEKIPATVVRVSDLWCGYGPDPVLKGISFGIHRGEFVG
                     ILGPNGSGKTTLLLALTGILSPSSGSVEIFGESLATLGPKSRARKMAAVSQDADVRFP
                     FSCEEVVHMGRYPHQKRWRMDSPEDAAVVDRALEITETTPLAPRLITAVSGGEKQRVT
                     MAKALAQDTPLLFLDEATSAMDIHRKLQIFGILERLNRETQLTVLAVLHDVNLAALYC
                     DRLIFLKEGNLAADGPTASVLTPEILEELYQTRVRVYPVEGTDKRQVIFLP"
     gene            complement(29731..30783)
                     /locus_tag="PLM79_05615"
     CDS             complement(29731..30783)
                     /locus_tag="PLM79_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV85818.1"
                     /translation="MTSPLSHSLPRWLGWIGLCLLIALAAFVTSGMGPFPIPYETIWR
                     IWISPLDPSAAQGIDDTTRYIAVHVRLARVCLALVVGSALALAGAVYQGVLLNPLADP
                     FTLGISAGAAFGASLCIILGWGGTLLGNVNPLPAAAFAGALGALYLVYMLGRMDGRLH
                     ATTLVLAGIVVSTFLSAWISLLKSLNEDSVSTIVFWILGSFSGKSWTQVAVVLPYLIL
                     GGTAILYYARELDILSLGDIQAQQLGVDVARVRFHLLLAASLITAAAVAVSGIIGFVG
                     LVTPHLVRLAIGPRHRKLLPAVLLGGGLLVLLSDTLARSALPNGEEIPVGVVTAILGG
                     PFFCFLLMHRKKYLQL"
     gene            complement(30813..31550)
                     /gene="cobI"
                     /locus_tag="PLM79_05620"
     CDS             complement(30813..31550)
                     /gene="cobI"
                     /locus_tag="PLM79_05620"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HOV85819.1"
                     /translation="MNEEQAMLGTLYGIGVGPGDPELLTLKAVRVLKAVSHVFAAASS
                     KNDYSLALEIVREHLPPETPVDHLLFPMTFHAESLENAWTANCERVVQILGEGKHVAF
                     LTIGDPLTFSTFIYLMRKIRNRLPGVKIVTVPGITSFQAAAACATVPLAEGEEAITII
                     SGAKGGDRLKEAIRVSENVVLMKTYKQFPQIVSQIEEAGLTDSCCFVSRCGLDGQVVE
                     RDFKRVCEIQQPHYLSLMIIKKRGLEP"
     gene            complement(31543..32229)
                     /locus_tag="PLM79_05625"
     CDS             complement(31543..32229)
                     /locus_tag="PLM79_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HOV85820.1"
                     /translation="MSHSSESLKTDRPGLVEAGRRIEVESFRIIDREMPDHSFSPPQW
                     EVVRRVIHATGDFDFARWFRFHPRAVDAGIQALRAGASVFVDTRMIHAGLSPWRLGWF
                     GNEVVIPAVHPDSHEWGQAMGTTRSVAAFRYVSRRMDGNIVTVGNAPTALLEVVRLIR
                     EEGLRPGLVIGVPVGFVRADESKEALWDLPDQPNITVLGRKGGSTVAVAVLHALLELA
                     RHAREEEAGE"
     regulatory      complement(32517..32713)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            33057..>34504
                     /locus_tag="PLM79_05630"
     CDS             33057..>34504
                     /locus_tag="PLM79_05630"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOV85821.1"
                     /translation="MSSWKRWLWPLAGGFIGLGTLGFAVSFTSASYLVDLWWFRSLGY
                     EFYYWQRLLYRYVIFGVVTLLFFLIFFLNFRVASRHLGSTVPPTSGARPSSIRAYSDL
                     LRLFRTGSMWVYTPLSLILAVPIALPLFQRWEDFLLFVFAPSAGLQDPVFGKDVGFYL
                     FSLPIYSLLQKRLFLAFLLLLHGLLLLYWLERRLLVRRDERMPRGAKWHINSLVLLTT
                     LLGVGVFALQRYELLYAETHEPLFFGPGYEEMKVILPMIWMVMVLLLATAALLVHFIY
                     TRKDWRPLAGAAACLCLVLWGRYSDFLPSVVNRYIVKPNALSLEKPYIANSVSATLAA
                     YDLDRVEVRDFSPERMPRGVPEPKVQDILRNVPVWDGELLTNVYEQLQELRTYYDFSG
                     VDVSLYEVRGNLQQVFLAARELNADKLPSGARNWVNEHLTYTHGYGVVMTPAAQGGDE
                     PITWFIRGIPVESEFGFNIRQPGIYYGELDHYR"
BASE COUNT         6426 a        10560 c        10714 g         6804 t
ORIGIN      
        1 tccggcgaag ggcgtccagt tcggtgatgt ccgccgtttc ctcgtggctg acgtggggaa
       61 tttgcgacca tgagcgggcc atttggcggg aagtcgttcg ccgaacggat cgaaggggca
      121 tgcgctcgac ggttgccgga aacggggctt ccggcgatgg ggcggccatc tccggttccg
      181 gcgggacttg tgggggcgca cccgtgggag gtgccggttt cgggggcgcc tcgggagccc
      241 tcttcacaga ttccgcatag gctcgaacgt cctcggacgt cacctgcccg gcaggacccg
      301 tcggggtcac ccggcgaaga tccaccccca gctcccgggc cagccgccgg gtggacgggg
      361 aggccggaac gggcccctcg gaggtgggag gcgccgccga aagatccttt gccggggatc
      421 cgaccgccgc cgcttccgtc ccgccttcgc cggggagcac tactgtctgc ccggtctttt
      481 ccccgacctg gaccccgaaa gtcggccctt tcgtgtcccc ctcctccagg aaggtgatga
      541 gcacactgcc gacctggacg gtatctccgg gtttgacccg gatctcgtga acggtccccg
      601 tgaccgggga agggatttcc gtggtggcct tgtccgtttc caccaccagg acggtttgac
      661 cctcccggac cctgtcgcct acggccacca gcacctctac gatttcccct tcgtgaatgc
      721 cttcccccaa atcgggaagc ttgaattcct tcggcatacc tcctccttaa cggttcaaaa
      781 cggcctttca acctcgacca tgacctagga gtccaggact tggtgaacgg cccggacaat
      841 ccgcggtgcg ttgggcagat actgctgttc ccgttgaaag aggggaatca ccaggtcgta
      901 gcccgtcact cgccgaacgg gagcctccag gtacagaaag gatttttcca ccaggcgggc
      961 caccacctcg gcgccgggac caaaggagcg gggcgcctcg tggaccacaa ccacccgacc
     1021 ggtcttccgg gcggaggcgg caaagagctc gtcgtccagg ggcgagaggg tcagaaggtc
     1081 gatgacttcc gcctcgattc cctccttgtc gtgaaggtcc gaagccgctt cgagggtggg
     1141 ccgcagggaa gccccgtagg agatgagggt gacgtgcccg ccctcccgca ccaaccggga
     1201 tttcccgatg ggcagggttt cttcctcttc ggggacctct tcccggaaag cccggtaaac
     1261 ggctttgggt tcgtaaaaga ccacggggtc cgggtcgcgg atggcgctga cgagcagggc
     1321 ccgggcgttg cgcggtcccg acgggacgac cattttcagc cccggggtat gagcccagta
     1381 ggcctcccgg ctttccgaat ggtgctcgag ggcccgcacc ccgcccccgt agggacaacg
     1441 aagcaccatg ggcacgtgga agcgtcccat ggacctccat cgcatgcggg cggcgtggga
     1501 ttccatctgg tgcatggcaa agtagctgaa tcccgaaaac tgcatttcac agacgggacg
     1561 aagcccgtag acggccatgc cgatggacat cccggcaatg gcggattcgg ccaaaggagt
     1621 gtccaccact ctctcgggtc cgaatttctc gatgagccct tccgtcacgc gaaagacccc
     1681 cccgtccacc ccgacatctt ctcccaggac gatcactcgg tcgtcctttt ccatttcctg
     1741 ttgcagagcc aggttgatgg cctgcaccat gttgagcttt gccatggccg tcctctcctt
     1801 ttatcccgga tggaagccga cgggaagcgc ccgactccca tggttggtcg atgttttggg
     1861 gctgatcctc acgatcccgc aatgccggga gcctcgacag ccccccgagt ccggccggcc
     1921 gccggaccct ccatgccgtt tccgtccttt gtgtctttcg tgctcttccc gttattcggg
     1981 ggaaagaccg acgatggagg cgccctcagg agcctgcctt tccctgctgc tcccgcagct
     2041 cggccgccag ttcggccttc tgctcgagca cgctcggagg cagctccgcg taggcatggt
     2101 cgaacatctt caacgggtcc aacggcccct ggatcctctt ctcccatcga gcctcgactc
     2161 ccttgatgtc gttttgtacc tgttcctcga tggactggac ggtctcttcg gtgaggagtc
     2221 ccttctcgat caggtatttt tgaaatcgca ggatgggatc ccgcttggcc cactcttcga
     2281 cttcttcatc cttgcggtac ctcttggggt cgtccgccgt ggtgtgtacc gacatgcggt
     2341 aggtgacgca ctcgatgaag gtggggcctc ccccggcccg ggcgcgatcc acggcctccc
     2401 gggccgccgt gtagaccgcc aggacgtcgt ttccgtccac catgatgccg ggcatatcgt
     2461 aggcgagggc cttttgggcc agggtggacg agtggctctg gcggctgcgg ggtatggaga
     2521 tggcccagtg gttgttctgg cagacaaaga ccaccggtgc ccgaaacacg gcggcaaaat
     2581 tcatggcctc gtgaaagtcc ccctcggagg tggctccatc cccaaaaaag gtcatgacca
     2641 cctgctcctg cttgcgatac cgggccgcgt aggcgaggcc gacggcgtgc agcgtatggc
     2701 tgcccacggg aatggagatg ggcagattgt tgactcccgg gggaatgact cccccttcat
     2761 tgtagccgcc gaaaaggagg agaagactct ccatatcctg cccccgccag acctgggcgg
     2821 ctccctcgcg aaaggaaggg accatccagt cctccggttt cagagcggcc acggctccca
     2881 gttgggaagc ctcctgcccc ttgatgggag caaaggttcc gatacgaccc tggcgctgaa
     2941 gtatcagcat tcgttcgtcg aatctacggc ccaggatcat ggcccgatag agcctcagaa
     3001 gaagatcatc gggaaggtcg ggttccaggc tcttgtccag gactccgttc tcatccagga
     3061 tcgagagggt ttccacccgg tgagggagat cgatctcttt tctcggcatg accagtcctt
     3121 tcctcgtgga aatagggaaa tcaagcggtt tgggaaacgg gagtccttgc cgaagattcg
     3181 gaagacgaaa gaggaaccgg agtgccgaac tcccgggatc ccgccggacc cctgtgtgtc
     3241 gggatgaaga cgacccgcgc ccatggggcg gcggccgggc caaaaacact actaatataa
     3301 aagtgacgat gggtattggc tagaagaagc cgttcttttg ttgggaagag gcatcgggtc
     3361 cgatgcctac cgttggacaa gaccgggcaa taggaccgtc accccgccca cgagaacctc
     3421 gacggcaatg gcggccaata tcaaccccat cagtcgcgtc acgatgttga ttcccgtcct
     3481 tcccagggcg tttccaatgg ggatggcgag ccgaagggtc aaccacacgg tgagggccac
     3541 caggaggcac acggccagga gcagccccgc atgcttccag tccggcaact ggtgagcgta
     3601 gatgatgacg gtgctgatgg ctccgggacc tgaaagcagg ggaacggcca gggggaccac
     3661 ggcgacgtcg gccttttccc ccgcttcggc ggcttcttcg ggagtctgct tgctggcgct
     3721 gggctgcgcg tggaacatgg caacggcgat gagcagaatc aggatgcctc ctcccacccg
     3781 aaaggaagcc acgctgattc caaaaaaccg caggagggaa tctcccagga ggcacgccgc
     3841 catgaggacc acccccatgg tgacggaact gaccagggcc gtgcgccgcc gttccttggc
     3901 ggactggtga cccgtcaggc tgacaaagat gggcagaacc ccgatgggat tcacgatcac
     3961 caggagagcg gtaaagattt tgatatattc ggtccagacg aacattttct cacggccgtt
     4021 tccaaatggg ttcgacaacg gcacggcccc ccccggcgat ctccggcggt gcggggccga
     4081 ccatcctccc tcccgtgggc tctcccccgg aagggtcatg cttaacatac tggtattggt
     4141 cgatcgagaa aagggctttc ggatcggacg gcagggagga gcgttcctgg gtcccggggg
     4201 tacacgggaa caggaattgg aagggaaaag ggatgaagag actgcagcgt ctccatccgg
     4261 acctgcccat ccagcatttg aagccggaaa agagggcggg gctcgtggtg gtgatcacgg
     4321 gaaacggaaa ggggaaagac ctcttcggcc gtgggcatgg cggttcgggc ctgcggccac
     4381 ggcatgaaga tttccatggt ccagttcatg aaagaggatc tctatacacg ggggagtggg
     4441 acggcctggg aaagctcgag tgcgacgtcg aggtcattcc cacgggaatg ggttttgcgg
     4501 gatccagggg accccctttc cctacgagga acaccgaaag gccgctcagc aggccgtaga
     4561 aatcgcccgg gaaaagctga attccgagag ggtcgatctg ctgattccgg acgaaatcaa
     4621 caacaccctg catcttcggc tggtagacct ggagcaggtc ctggacctga tcggcgcaaa
     4681 gcctccccgc atgcacctgg tgctcacggg gcgggacgca cacccaagag caatcgagcc
     4741 ggcggacacg gtgagcgagg tggtcgagat caagcacgcc tatggaaaag ggatcgaacc
     4801 ccagccgggg atcgattcct gaggccgggg aaggtgcagg gcctttgggc ggacaagccg
     4861 ttgcctgagt tgagcgattc cgagagcatc cgggcgtcat gcccgccaag gcgggcatcc
     4921 attgggtatg cacgctcttt ccggattccc gcctgggcgg gaacgccgaa acaagattcg
     4981 tcccggtcaa atcgctcgat ttaggcctta tttgccgacc tgggacaggg cttgatccag
     5041 gtcccggcgc aaatcttcga aatcttcaca tcccacggag agacgcacca gctcatccag
     5101 gattcccgcc tgctgccgtt cctcccaggg cacggccgcg tgggtcatgg acgccgggtg
     5161 ctcgatgagg ctctccacgc tccccaggga gaccgccagg gtgatcagcc gcacgcgatc
     5221 catcagggtg acgccgccct catagccgtt tttcaccccg aaacagatca tggagccgaa
     5281 gccttccatc tgctttcggg cgacggcatg ctgcgggtga tccgggaggc ccgggtagcg
     5341 cacccaggtc accttgggat gcccggcaag gtattgggcc aggcgcaggg cgttttcctg
     5401 ggctcgctcc atgcgcaacg ccagggtccg cagcccccgg aggatgagcc aggcctggtg
     5461 agggtccatg gtggagccgt acgtgctgct tgccttgcga agccgggaaa ggagctgggg
     5521 gtccttggcc gtgaccatgc cccccaggac gtcgctgtgg ccgttgagga acttggtgag
     5581 gctgtggacc acgatatcgg ccccccaccg aaggggccgc tgcaggtaag ggctggcgaa
     5641 ggtattgtcc accaccagga gggctccgtg ttcgtgggcc atacgggcgg cggcttccag
     5701 gtccgtgatc ttcatggtgg ggttggtggg agtctcgatg aacaccagcc gggtttcggg
     5761 acccagggca tctcgaagga tctccgggtt ggaggtgtct acgaaggagg tgcggacccc
     5821 gaaacgggaa aggtccgatt ccaggaagag gtgagtggcc ccgtaaatgg aatccgtgga
     5881 caccacgtgg gccccttgtt cgagaagggc caggaacgtg gtggtgagcg cccccattcc
     5941 cgtggacgcc gcaacgcccc cgctgccgcc ttccagggag gcaacggcct cctcgagaac
     6001 ccgcaccgtg gggttggcca ggcgggtgta gatgaacccc ggcctctcgc ctgcaaaccg
     6061 ggccgcccct tcttcggcgc tgggaaaggc aaaggtcgag gtttgaaaga cggccgggac
     6121 cacctccccg tagaccgagc tcttcactcc ccccaggtga acggcccggg tggatttctt
     6181 tctgtcctcc atcgaaggat ccttttccat catgactcct gtgcgcgggt caaagcaaac
     6241 ggtgcctatc taacgaagac gatgataccc atggtggagc gactttcccg gttgcaaggg
     6301 tgtgcaaaaa gcccgcccct gcaggcggac cgtcctgcag gggccggacg ccgtccccgc
     6361 ccgccgggat cgccggtgga cggggaaatg gccgtcaaga gtccctcggt ttgaattcca
     6421 tgtgcgccgg tgggcctccg ggatgcaagg tgctttcggg gtcttctccc cccgggaaga
     6481 ggtctgggga atcccgatat ccccccggcg gggatggaac cgttccggcc ttccccctct
     6541 cccaccggat gtaactacca caatttgggg gtcaagggag agggggtttt ccgggagttc
     6601 ctctcttcct tgacaaagcc cgatgggcat tctaataatc atcgcaccgt cgtttcaacc
     6661 ggatggagcg gtttggggac ctttggccca ccccctgtcc acggaaatcg cccgatccgg
     6721 agtcctcggc cggtgctgcg aagacttgca agcagaccct caaccaggtg aagccatgcc
     6781 gatccactcg aggtcttttg ccgtcatcat ggggacggtg gtcttgcttt tcgtggcggg
     6841 atgcgcaccc gtgaagcagt tgcgggtcgc gaccgtggcc ctcacgcttc gggatgtggc
     6901 cgatgccgcg ggaaagcaat ccagtccccg ggtgattcgg gagggaaccc ccgcctacct
     6961 gatgctcctc gatggactgc tggaagccga gccccggagc cgggagttgc tccttgccgc
     7021 ctgccgggcc tatgtttcat atgcatcctc cctggtcggg cagggcaacc ccgaagaagt
     7081 gagcgtcctg tttttccggg gcagggattt cgggttccgg gccctgtccg agtctttgca
     7141 gacgaacttc aaggaagcct cctcgggcac cctggacggg tttgcggcag tgcttgcaaa
     7201 atgcgggaag aaggacgtgc ccgccctttt ctggaccacc aacgcgtggg ccaactggat
     7261 cgctctgaac acctcgaagg tcgaagccct gggggatctt cccatgctgg aagccgccat
     7321 gaaaaggacc ctcgagttgg atgaaaccta tttttacggg ggaccccacg tcatccgggg
     7381 gatctacctg gcttccaagc ccgccgtgtt ggggggcaat cccgcccagg ccaaggtcca
     7441 tttcgaccgg gcccgggagc tgtccggaga caagaacctt gtgcttaaag tctacctggc
     7501 cgagtactac gcccggtccg tgggagatcg agagctctat cgcaggtccc tcgaggaggt
     7561 gttggcggcc cccctggaag aagggagcga catgaccctc tccaatgtca tggccaaaga
     7621 aaaggcccgg tggttgctgg acaaaacgga ggagtatttt gagccggaac ggtagatcca
     7681 tcgtccgatg gtttaagggg aaagcgcttc ttccctgctt gttctccgtg cttacggggt
     7741 gttttgcggt catggggtcg gtttggccct cgcctgccct gggagccaag cccgaacatg
     7801 aaatcaaact ggccaccatg gcccccgaga acagctctct catgaagatc tttcacgaga
     7861 tgaacgaaga gctcctcaag gaaaccgggg gaaaggtggg gttcaagatg tatgcgggtt
     7921 tcgtgctggg ggacgaggag gacgttctgc gcaagctccg ggtgggcatg gtccatgccg
     7981 cggcctttac cgccgccgcc ctgacggacc tgagccccga tttgcgggtc tttcaagtgc
     8041 cgttcctctt tgccgactac aaagaggtgg acttcgttct ggacaggatg gagtcggacc
     8101 tcaagaaggg gttcgaaaag aaggggttct acgtgttggg gtggccggag cttggcttcc
     8161 tgtatttcat gtccacgact cccatctcga gcctcgagga cctcaagggc aagcgggttt
     8221 gggccaaagc caacgctccc atgctcgagg ggctcatccg gaaaaccggg gtctccgccg
     8281 tggccatcaa tgcaccggac gttctggtgg cgctccagac caacctcctg gacgtggtgt
     8341 acaattcccc ctattacgcc gtggtcaccc agtggtacac ccagttcaag tacgtgaccg
     8401 atctccccct ctcctacatc ggtggggcgc tgatcctgga caagaaggtc ctggacaaga
     8461 tcccagcccc tctccgggaa accttgcgaa aggtttgcga caagcacctg cgccgcctga
     8521 cggaacggac tcgaaaggac aatgcggaat ccctgcaggt gattttgaag cggggcgtca
     8581 cccagattac ccccaaggcg gccgaagtcg aggaattcaa aagagtgggg gatgaggctg
     8641 tgatggccct ggatcccaag tactttccca aggacacttt ccagaaggtg aaggtttggc
     8701 tttcccagta ccggacggct tcccccaatc ccaaatgacg gtcccgccgt tgaacccctg
     8761 ccggagagct ctccaccatc tccatgactt cgagaagtgg gtctttgtgg gcctgactct
     8821 ttttctggcg ggctttgccc ttcttcaaat catcctgcgc aatttcttct cgacgggctt
     8881 tatctggggg gataccctgc aaaggcacct ggtcctgtgg atcagtcttc tgggggcggc
     8941 tcgggccacc ctcgaggaca agcacatcca ggtgcttctg ctcccggtgc tgctgccccc
     9001 ccgggcggcc cgcttcctgc cctacctcag cgacggcttc agcctcgccg tttccgccat
     9061 cctcctgtat gccgcctgga tttttgtggg gaacgaacgg gcggcaggcg acctcgcctt
     9121 ctccgggatt ccctactggt ggttggaatt catcttcccc gtaagcttcg ccatgatgac
     9181 cgcccggttc gggttccgcc tctcggggag gctcttcggg gcgaaagggg ggaacccatg
     9241 accctcttgg cggttctggg cctgcttttc ttgaccctgt ccggggtccc tctctttgtg
     9301 gtcatctccg cggcggccct cctttgcttc cggtacctgg ggacggacct gtcggtggtc
     9361 atcatcgaaa tgtaccgatt ggccgccaat cccatgatcg tcgccctgtt cctgtttgcc
     9421 ctggccgggt acatcttggc ggaaagcaag gccagccgga ggttggtccg gctgtccacg
     9481 gcgctcctgg gtggcgcccc gggaggcctc gccgtcgtgg cgctgctgtc ctgcgctctc
     9541 tttaccgctc ttaccggggc gtcgggagtg accatcgtgg ccctgggagg gattctcttc
     9601 ccggccctca ccgccgatca ttaccgggag aacttttccc tgggcctgtt gaccacctgc
     9661 gggagcctgg ggctcctgtt tcctcccagt ctgcccctca tcgtgtacgg aatggtggcc
     9721 gaagccaaca tctcccacct gttcctggcg ggccttctgc cgggcctgtt catgatggcg
     9781 ctgttgggcg cctacagcat gtaccagggg acacggtttc aggccccggg gagccgcttc
     9841 cggttcagcc tcaaggaact ggtcggggca ctcaacgaga gcaagtggga ggccctgttg
     9901 ccggtggtgg tgctgggggg catcttcagc gggtatctcg tgatgagcga agccgcggcg
     9961 gttacggtct tctacgtctt cctggtggaa gtggtcatcc accgggagct gcgcatccgg
    10021 gacctgtcct cggtcattgt gaagacggtc atgatggtgg gggggatcat cgtgatcctg
    10081 ggggcgtccc tggcgttcaa cagctttctc attgatcagc aggtgcccga ccggatcttc
    10141 gaattcattc agtcccgcat cgaaagtaag atcggcttcc tgcttttgct caacgggttt
    10201 ctcctcattg tagggagcct cctggatgtc ttttccgccc tcatcattgt ggttcccctg
    10261 attgttcccg ttgccgcgaa ttacggggtg gacctcatcc acctggggat catctttctc
    10321 accaatctcc agatcgggta ctcgacccct cccatcggga tgagtctttt cattgccagt
    10381 ctgcggttcg aaaaatccat cctgcagctc tacgtggcct ctcttccctt cctgtgcatc
    10441 ctcctggcgg tcctggccgt gatcaccttc gtgccgtcgt tgagcctctg gctggtgcct
    10501 tgaaaggaaa agcgccggta caggagaaga aatgcgcgcg cactatcttc agcatgttcc
    10561 gtttgaaggt ctgggaagta ttgaatcctg gctggattcg aatgggtacg agctgaccca
    10621 cacccgtttc ttcgagtcag tcgatttccc ggaccctgaa gccatcgatt tgcttgtggt
    10681 catgggcggt ccgatgagcg tgaatgacga ggatcaattc ccctggcttg tcctggagaa
    10741 gcggtttgtc cgtgaagtga tcgagtccgg caaatccgtc ttgggcatat gtcttggcgc
    10801 tcagatgatt gcccatgcaa tgggggcgaa agtctttccc aaccccgtga aggaaatcgg
    10861 gtggtttcct gtccgtgggg ttgcctccgt tgacgcctcg gtcttccgtt ttccgccttc
    10921 ggaaatcgtg ttccattggc atggagaaac ctttgatctg ccgccgggtg ccgtacggct
    10981 cgcaaagagc gaagggtgcg aaaaccaggc gtttcaactg ggaaagcggg taatcggcct
    11041 gcagtttcac ctggaaacaa ccccgaagtc ggcaagagaa attgtctccc attgtcgcca
    11101 cgagctggtt ccctcaagat atgttcagac ggaggaagaa attctatcgg caagcccgag
    11161 tcgctatcaa tcgatcaatg acctgatggg cggcgtccta tcctttcttc aacgaggcga
    11221 cggctgatcg atcggccggg ggttttccac cccgctccca caccacccgg ggtgcgggtc
    11281 cgcaccgggc ggttcatcgg gtttcccggt cctggaccgg ggaaggaagg ttcgccccca
    11341 ccgctcctta gtgaccggat cacgcccgat gacgagagtc cgctttttcc cccgcagatc
    11401 gatcgctgaa gccgcggggc tgcctctttg cgccgtggat gccgtcgagt cccgatttcc
    11461 accccacgat ccggatcccc gacccgaacc ttccaggcct ttcgttgatc ccttggcatc
    11521 gcgaccatag gacaatccgg tgtcccgccg tcggggccga gaaaccgtgc gtgcggctgc
    11581 ccgtggtcat ttttgaaaaa gatggacgat cccggggtca agaagacgga cgggtgggca
    11641 gcggttcttg atcgcgccgg attgcggccc gggaacgccc gcgggcggtg tatgattgcg
    11701 ggaccgcgca ccgagggggg cgaagtccgg gaccctccgg gagcgatggc ggaatcgcgg
    11761 ggttgggcat gaaattgctt gaccttcgag aggagttcta atacacggaa cgggtgtcga
    11821 gaaaagcgtc cgaaaattgg agacagcaca tggtaaagaa gggcattgac gacgatcacc
    11881 tgatggaaga gttcgacgag gacggcggcc tggagttcga tcaaacggac gagttggact
    11941 ttaccggaga ggatgatctg ctcatggagg aaggggacga agaggacctc atcccttccg
    12001 ttctctacaa aggggtgtca cgacccaaga cggatgaaga ctggcgccag ctgctcctcg
    12061 atgcaagcag agacggggtc ccggaataca agatgtcgga cacctacaag gaaggggacc
    12121 tgatcattca tccgtctttc gggctgggtg tcgtcagcaa actgatttct ccaagaaagg
    12181 ccgaagtcat cttcgaaaac agcaaacggc tcatggccat gggcgtgacg cccccccagt
    12241 gaggccccgc cgggcagagc ccgccccggt tagacggttt tcccgccctg ctcgcctgtt
    12301 ttcagggccg gggccaggtg ttcgccgatg cgctcccgca ggaacgggtg aaccaggggg
    12361 gttcccgcca cgatgtgacc gttgagcagg taggctccct caccccagaa atcgtccacc
    12421 cttccgcccg cctccaggac cagaaggctc cccgccgcca catcccaggg cgcgagtccc
    12481 gcttcccaga acccgtccac ccgtccggcc gccacgtagg caaggtccag ggccgccgaa
    12541 cccgccctcc ggattccgct cacctgccgg agtatggagc tgaaggcggc caggtacggg
    12601 tccaggattc gcttcatgcg aaagggaaat cccgtggcaa tgagggcctg ctccacggtg
    12661 aatcgttccc tcacccggag gggcgagccg ttcagaaagg ctccgccccc tcgcacggca
    12721 tgaaaaagct cttcccgtaa aggatccagg acgaatccca ggaggggttg caggccttcg
    12781 tagaccgcca cggacacggc cacgaaggga aacccgtgga tgaagttggt cgtcccgtcc
    12841 aggggatcga tgatccactg gatcccattg ccgatccggg ccaggttcgc ctcttcggaa
    12901 accaaacgat ggccgggaaa gcttctcgaa acggagtcca ggatgagggc ttcggcctcc
    12961 ttgtccacct cggtcacgta atccgaaggg cccttgtttt ggacggaaga cagggggacc
    13021 ttccccagcc gaccggcaat gagctttccc gccttgagag tcgccttcct ggcgacttct
    13081 tccatttctc ggatcaatcc cggctcaacc gacatgtgca ttcaccccta ttccccgttg
    13141 atcttgctcc ctcggatggg atacttagaa ggcagggtcc ggtgcgagtg gttttccgga
    13201 ccgggatcaa cataacggga gccatggggg aaagcaaccc ccgaggctgg gaatcgccgc
    13261 ccgttctttc ctcggaccca ggatggttcc atgaagatcg atgtgcacac ccatattttc
    13321 cccccggaga tctgccggga tcgtgggcgt tttttctcct cccagcccgc ttttcgcctt
    13381 ttgtacgact cgcccaaggc cccattggcc acggcggaaa ggctcctcga ggtcctggac
    13441 gaggacgagg tggaccgggc cgtggtgttc ggttttccct ggcagggtcg tgaacgagtc
    13501 ctgagacaca acgactatgt cctcgaggcg gcggcccggt atccccgacg actcattcca
    13561 tttgcctgcg ttcatcccct cgacccggaa gcccccgccg aagccgaccg atgttgtgca
    13621 cagggagccg ccgggctggg ggagctgggc ctctacgagc cgtgcgaccc ggccgccgcc
    13681 cttgccgcct acgacggcat tttccgggtg gcaaggtcct acgggaagcc tctcctggtt
    13741 catgccaacg aacccgtggg gcacgcctac cccggcaagg ctccccaggg cctggatttc
    13801 tactatcgac tggcgcaacg ggccgaggga attcccctca tcctggctca ctggggcgga
    13861 ggattgtgct tctatgaaat gatgaaaaag gaagtggccg aaaccctgtc tcatgtcttc
    13921 tacgacacgg cggcctcccc gttcctgtac cgtgcatccg tgtaccgcac ggtcctggac
    13981 atcgtgggca aggaaagggt cctgctgggg agcgatggtc ccctcctgag gccccggcgc
    14041 tatttccacg agatgcaggg ggttctgtcc ccggaagaga tgagggccgt gtgcggggag
    14101 aacgcaggga ggcttttgtc cggtgcccgg gacgacggct tcgagggacc ttcgctaacg
    14161 gatgcatccc cctgagacgg ggtctgcagg cgggccgttc gaacccgcta ccatgtggaa
    14221 ggagcctgca tccggagttg tgtctccttt tgaccgtcgg tcccctgtgg ggcaaggttc
    14281 aagaagggtt tgatcaaagg gatgattttt tgtacaagga accgttttct tatcccggga
    14341 cccccgacgg atgtcggttc cggaggggtc acggagcggg agcccggggg aagggtacgg
    14401 ggttggcaaa cgtgggtcaa gggcaagaga gtacttcaat cgtggaaagg gtccctttcg
    14461 gagaggagga aatcgcggtt ctgagggagt ccgtagagat ttccgaggag ctcatcagcg
    14521 atttcttcaa gatctcgacc tccgaatgga agcggtatcg ctacgacatc cagtccctca
    14581 aagacctggc cgacggggaa atcaccgacc atgcctttgc ccagatccgc cgctatcaga
    14641 ggtctcctca cctgcgactc cggggaagcg aaccgggaga ctacttcaag atctgcctgc
    14701 aggaccatgt cattcggcag gccatagtcc gggatgctca cgtccgtctc tttcccctca
    14761 cggcctacat tgccacccac gagctcatcc acgtggtgcg gtttgcacgg tttctgcaaa
    14821 gcttcgacag cactcccgca gagcaactgg cggaggaaaa gcgggtccac gtcctcacgg
    14881 aacagctcct ggcccactgc aagatcccgg gccttgccga ggtcctgggc gcctttcggg
    14941 aatgttacac catggaaacg ttccacaagg cggcggattg aagtggaagg atgccgttgc
    15001 cgccacgcgc cgtgagatcc ggctttcgcc ggaattcggg aaaggaacgc gggttcgcat
    15061 gaagatcgtt gcctcatccg gattgaactc ctgacaccgt tggaaagcgg ggattcccat
    15121 gaaggaaaag atcacgaggg aagaggtgcg ccacgtggcc gatctggcgc gcctcgagtt
    15181 gtccgaacag gaagagtcga gaatgacgga acagatgaac cagatcctcg gctacatgga
    15241 ccagttgaac gagctgaaca cggagggggt ttcgccgacc acccatgcca tcgagctgcg
    15301 aaacgtgttc cgggaggacg aggttcggga atctctgccc cgggaacagg cgttggccaa
    15361 tgcgccccaa agcgacgggg tgaatttcct ggtccccaag gtcatctgat catcccaccg
    15421 aaaggtttct tccatggatt tgaccgctct gaccattcat gagttgcgcc gacgactggt
    15481 tcgcagggaa gtgagcgtga ccgaggtggt gcgccgttat cttgaaagaa ttgaggcctt
    15541 ggaccccagg gtgaattcct acgtgaaagt gatggccgac gaggctttgg aggaggccgc
    15601 gcggtacgat cggggagaaa aggacctcga aggccctccc ctggccgggg ttcccctgtc
    15661 cgtcaaggac gtcctctgca cccgcggaat ccgcacctcc tgcgggtcca aaatcctgga
    15721 aaactacttg cccccttacg acgccacggt catcgaaaaa ttgaaagcct cgggtgccgt
    15781 cctcctgggg aaaaccaaca tggacgagtt tgccatggga tcgtccacgg agagctccgc
    15841 cttcgggccg acccgcaacc cgtgggacct ggatcgagtg ccgggagggt ccagcggcgg
    15901 ttcggcggcg gccgtggctg cggacctgtg cgccgggtcc ctgggaacgg acacgggggg
    15961 atccattcgt cagccgggtt ccttctgcgg ggtcgtgggt ctcaaaccca cctacgggcg
    16021 cgtatcccgg ttcggactcg tggcctttgc ctcctccctg gatcaaatcg gacccatcac
    16081 caaggacgtt cgggacgcgg ccctgctgct ccatgtcgtg gcgggttacg accgtcggga
    16141 ttccacttcc gtggatctcc ccgtccccga ttatccggag tgcatccggg agcccgtgga
    16201 cgggcttcgg ttggggattc cccgggagta tttcgtggag gggatgcacc cggacgtcct
    16261 gtcccgggtg cgacaggcca ttgaaacctg cagggaactg gggggggagg tggtggaggt
    16321 ctcgttgccc cacacggcct atggagtggc ggcctattac atcattgcac ccgccgaggc
    16381 gagctcgaac ctggcccgct acgacggggt caagtacggg actcgggctt ccgatgcccg
    16441 ggaactggcc gccatgtacc gggaaacccg gtcccggggc ttcggacccg aggtcaagcg
    16501 gcgcatcatg ctgggtacct atgtgctttc ggcgggatat tacgatgcct actaccggaa
    16561 ggcttcccag gtccggaccc tgattcggca ggatttcctg gacgccttcc aaacctgcga
    16621 cgccctgctg acccctgtgg ttccaacgcc tgcttttcgc gtgggggaga aaaccggcga
    16681 tcctctccag atgtacctga gcgacgtgtt caccctcccg gcaagtcttg ctggaatccc
    16741 gggaatctcg gtgccgtgcg gttttacctc ggagggactc cccgtcgggc tccaaatcct
    16801 ggggcctcat ttccgggaag acctcgtctt gcgaatcgcc cgccatttcg aggaggctgc
    16861 gggcttttcc cggcgaaaac cggacctttg cgcctagtcc gcagcggaag aagggcctaa
    16921 gagtcgggct gaaaatagcc cttgggaacc accacgattc cccggggggt caccacgaat
    16981 cgctttctgt cttcggcggg atcgatgccg atctgggtgt ggggcgggat cacattgtgc
    17041 ttgtcgatga tggccttctt gatcctgcag tggcgtccca cctccacgtt gtccatgatg
    17101 acggattcat ccacttccgc ccagctgcgg accatgacgt tgtaggatag cacggagctg
    17161 cggacggtcc ccccgctgac gatacacccc ggcgccacca gcgaatccac ggcggtcccc
    17221 acccggggat ttttcccttc ccgctggttg aaaacgaact tggcgggagg atactggcgc
    17281 tggaaggtcc gaatgggcca cagggaaccg tacaggttga agaagggatc gacccccgtc
    17341 aggtccatgt tggcgttcca ataggcgtcc agggtgccca catcccgcca gtactgggaa
    17401 tcccgggtct gttccgtcag ctgcattttt cgggagcctt cgggatccat caggcgaatg
    17461 tagtccttga tacggttcag tcttcggtag ggataggcga taattttcaa ggtttgcagg
    17521 tagttgggga tgatgtcctt cccaaagtcg tcgtagtccg tcttcttgag caactccagg
    17581 aggacctcgg tcttgaacag gtagatgccc atggaggcca gggaccgctc gggatcgtcg
    17641 ggaatgggct tggggtcctt gggcttctcc tcccacgcca cgatgcgaaa gttgttgtcc
    17701 acctcggcaa tgccgtaacc ggacgcggtg cttcgctcga cttcgaggag agccacggtc
    17761 acgtcggcgc cctgctgctc atggtattcc cgaaagatgg tgtagtccat cttgtagacg
    17821 tgatcgcccg aaagaatcag caggtggcgg ggcctctccc gctcgatgag aaagaggttc
    17881 tgacggacgc aatcggcggt gccccgatac cactccgccc ccaagcgttg ctggggggcc
    17941 gcaatccgga tgtagtggcc caggtccata gaaaagatgt tccagccttc ctcgatatga
    18001 tccaccatgg actggctctt gtattggggg aaaatcacga tcttgtaaag ctgggaattg
    18061 atgcagttgc tcaggggaag gtcaatgagg cggtagaccc ccccgaaggg cactgcgggt
    18121 ttggagcgat cccgggtgag gggcatgagc cgctcaccgc gtccacccgc catgatgact
    18181 gtgatcacat ccttcatagg gacctcccat cgaagattga gtcggggtgc ggcaccgagg
    18241 ccgacggctt cagggcccga gggatccaag ggttcaaagg gttacttgag gtatttcttg
    18301 gggtccgcag ggaggctgtt gccccagtac accatgttcc cttcggtatc cacaaggacg
    18361 aagtgaggga ttccctgcac acgataggac cgggtgacct ttccggctgc gtcgaagagg
    18421 acgggccagg gaacggggtg tccctgctgg tagcgtttca gccgatcgag ggtgtcgccg
    18481 ttgcccacgt tgattcccag gatctccatg ctgtcccggc cgatcttgtc gcgaagtttg
    18541 gcaatttcgg gttttgccgc caggcaggaa ggacaccagg tggcccagaa atagagcagc
    18601 accgggcggt tgccccggta cgcgctcaat cgcacctggg acccgttcag gtccggaagc
    18661 tcgaaatccg gagcggcctt cttgtcttgg gccagccaag acgccaagcg gatatcgtcc
    18721 ggggaagcct tgacgtcgtg ggcaccccaa aggagcggca acgccgccgc gcacgcgatc
    18781 aagatccacc aggacgtcat ccgaccccga ttcttcttca tagccacaac tcccctgttt
    18841 tgatgaaata gtactcggcg gcaaggacca tgatcagccc gaagaacttc tggacccgcc
    18901 gggtccagac ccccgaccgc ggcaaggaac tcaaaagtcc cgtgaaggta cccaccagga
    18961 tcaccaaagt gcccaggccg taggagaagg caaagagcag cccgatgccc cacgcgacgc
    19021 tctggcgtgc ggccaccagg gtcaacagga ccgccaggat gggtgtggtg caggtactga
    19081 ccaccagggc cgaagcgccg cccagcaata cgctggtaag tatatcgtgc ccgggaaggt
    19141 cccgcacctt caaccggttc aagaacgcgg ggggggtgag cggaaccgcc tccaacatgg
    19201 ccagaccgaa gaacaggcac aggtttccaa cgagcagata ggtccatcgg gagttggtga
    19261 gtgccccgaa gagctggccc gacaaagcgg caaaggcccc cagggccgaa tagacgacgg
    19321 ccagccccag gacataaaag acggaaaggg taaagccccg ccaccggctc ccgcacgcct
    19381 tgcctcccac aaaagccacc gtaatgggaa gcatgggata ggaacaggga gtcaggctgg
    19441 ccaggacgcc gcccacaaag gccagcaaaa gccccaccat ggggtattgt tgggcgattg
    19501 tctcaaactg tcccagccac tccaaggatt tccctcattg ttcgtgattc atgggtcctg
    19561 cgtcgatcgt acctggtcca ggaggtgcag gctcttgaga tccttccgga tgtacccctg
    19621 aacccatccg gtcaaagccc tcaggaccga cattttccta ccctgagcca cccggagtct
    19681 ccagagtcgg tccaggggaa ccgtcctgct ccggccgatg agcatcaggg tccccaggtc
    19741 cagggcaagg gcttccgaag ggaagggcga atgctgcgga cagctcaggg tccctcggtg
    19801 cagatcccaa ttccatcgcc ttgcccggct caacggtttc ccacacaccc cgcaggcctc
    19861 cagggacggc aggtagcctg ccaggtcgag aaaacgcagg agaaacaaca ctaccacatt
    19921 tacggggtct ttgtccccgg tcaatcgccc gaaggtttgc cggaccagtt cgaaaagctc
    19981 gtcctggggt tccgcttcgg gaaccatctc gaggaggatt tccgatacca gggctgcgta
    20041 tccccatcgt tccacatcgg tcctgaggcc caggtgcggc tcgataagct tgcagccttc
    20101 gatccacgcc agcgacttgc gcgcccgata ggttacctcg acgaggctcc ccggctcgaa
    20161 agcatggacg aagcgttttt tgctgcgcct tgctcctttg gcgatcccct gcaatctccc
    20221 ccccgtgcgg gccaggaagg agacgagacg gtcggattcc ccgtagtctc tggtctgcag
    20281 aatcatggcc tcggtggtgc gtgtggtcat tgggggagtg gaagctttcc gccctccctc
    20341 gaggggaaag cctgcttgct cccctccctt gacgagagga gccggaggag ggtgaagaag
    20401 cgggtcatgg atccgtccta gaaactttgt cggccggcgc atgcttgtct ctgcgccctt
    20461 ggattcacga atcgaaacgg cttttcggtc catcccgcac cgggtgcgcg ggaacccgga
    20521 cttctcggtt ccggcggctc ccggcaaaag cccccggggc cgaccgtttt cccatgggcg
    20581 cttctatcgc ggttttgaag tcttggcggc ggcgtaaccg ggcaggcgga acttgagcac
    20641 gcttccgggt ttacccttga tttcgagggg tttgccttcc cacatcatcc ggactccccg
    20701 gggatttccg atcacgacct tcatgtcttt tcgggcttcc catgcgcgct tttctccggg
    20761 catcagatgg aggttttctt ccgccttatc gtccacccga acctggaccc aagtcagctc
    20821 cagcgcttcg acctcgaaac gcttgagggc ggcctccccc gtcgccggtg ccgggtactt
    20881 gtccgaaggg gctcggtctg tgggaggcgg tgaaggggcg gatgtcccgt cgggggcttc
    20941 gggactctgc agggagacga cggcgggtcc gccgctctta ccggtcgaaa cgccgggtcc
    21001 cgccgatgga tcgagggtcc tcgactccga cggcttcacc tccttgtgaa gtcccgcctg
    21061 ggcaggggca agggtcccct ggatgaggtc ggggggcagt tcctgctgag gattcacgtc
    21121 gggacccgtc tgctgcagga cggccaggcg ggcaagcctt tccgagtacc acacgccccc
    21181 gtaaatggct gcggccaaga ggagggccag gatcagcaga accaggatcc ccacccgtct
    21241 ctttcctctt agggaccgca tccatatgcc gtagcgccga attccctcct cctgcttgtc
    21301 ctgggctcgg atctccgctt cgtatcgttc cagcaagggc gcgggatcga tccccaacga
    21361 ggaacaatag gctcggatga agctgcggat caggagggtc gttccgatcc ggtcgaaatc
    21421 tccttcctcc aacgcctgca gcatgccgac gctgactcgg acctctcccg acatctcctt
    21481 gagggaccgg cctcgcttca atctctccga cttgagatat tccgaaagct ctttcatgat
    21541 cgcctgccct gcgctggagt ttagagggta atctgaatga aggagcggct ttccagctcc
    21601 ttcatccaat tctcatactg ccggttgacc tctttctggt agagctccct gcggattctc
    21661 tccttgttgt ccatctcgcc cgggtccggt ctttccgttc gaacatccag cagcttcacg
    21721 atgtagaccc ccgccggggt cactacgggt tcactgaaat cgttcttctt gagctttctg
    21781 acggcggcgg cgatgggagg agccaggtcc ccgagttcca cgaaacccac gtcaccgcct
    21841 tcctcggagg cgggaccctt ggaatattcc ctggccatgc ggccaaaatc cgagccgcct
    21901 ttcaaccgat tgtggatttc ctttgcctgt ttttgggccg cctgggcctg tttcccgtcg
    21961 tcgcccgaag gcagaaagat gacactgatc cgcaggcttt ccctggtggg taccgtgggg
    22021 ggcggggccg tcctcacgta agcattcacc tgttcatcgg tgatgacgat cttggacttg
    22081 aggacccggt ccatgaggcg actctgttcg atctgcttct tgacggtgtt gcggaagctc
    22141 ttcaaggttt cgccttcctg gcggagggcc tcctcgaact gggcatcggt gacgtggtgt
    22201 tcggctttga ggttctggat ggccgaatcc acctcgccgt ccgacacggt gatcttcaac
    22261 cgcttgatct ccgcctcggc aagcttctcc cgaatgagtt gctgcagcac ctctcgttcc
    22321 gaaattgcct cgggggcgat cccggggttg gccttcttca gcttttccat ggaccggatt
    22381 cggttttgaa tatcgctgta caggatgatc tctccgttga cgttggcgac aatcctgtct
    22441 acggtttcgc cgaacgcaac cccggaacac aggaacaaag caaggcacaa gaagattatt
    22501 tccttaatct tcatgggatt cccccaagga caaatgtggt ctaagttgct gaattgactt
    22561 ggtagcacta gcattttcct ccacctcttg caagatcctt ttgaatcggg caaggcggtg
    22621 agggggatgt ttgccccaaa tctccacccg gaatcgcccg tttgtccccg gagaaaccgc
    22681 gcatttcttc tgctccagga cctggagaaa acgctgcgaa tccgccgagg gggccaactg
    22741 aacccggaag ccttcctcgt ctgcctcgat gcgggccacc ccgaggcgtt tgaaaagaag
    22801 acggcatttt gccaggagca gcaggttgcg ggtactgtcc ggaaaaggcc cgtaacggtc
    22861 ccgccattcc gtggccaggt cgtccacttg ggcctcccgg gtcagggacg ccagcctctt
    22921 gtaggccagg agcctctggt ccgtatcggc gatgtaggag tcgggcagga aggccgaaac
    22981 gggcaggctg atttcgggat ccacggtctc gggttccaca ggttctccct tgaattcccg
    23041 aatggtcttc tccaggagct cgagatacag ctcgtagccc accgccgata tgtgaccgga
    23101 ttgggccgaa cccaagatgg tgccgcctcc ccggatctgc aggtcgttga gagcgatctt
    23161 gaacccggcc cccagttcgc tgaaatccag cagggccctc agcctcttct gggcgtcccg
    23221 actgatcagg tgttctccgg gaatgagaag gtaggcaaag gcctgctcgc tggaccgccc
    23281 cactcggccc cgaagctggt aaatctgggc cagcccgaac ttgtccgccc ggttgatgag
    23341 gatggtgttg gccgcgggga tgtccaatcc cgattcgatg atcgtggtgc agaccagcac
    23401 atccaccttt cgatgaatga agtccatcat caccttttcc agctcccgct ccttcatctg
    23461 cccgtgggcc actccgatcc gggcatcggg gatccattgg gagagccggg cggccacctg
    23521 gtagatggat cccacgtggt tgtgcacgaa gaagacctgt cccctgcgct ggagctcccg
    23581 gtgaatggct tcccggatgg taaattcgtc gtaggcgcac acgtaggtct cgatggcccg
    23641 tcggtcctgg ggaggggtct cgatggtgct gaggtcccgt atgcccgaaa gggacatatg
    23701 gagagtccgg gggatgggag tggccgtgag ggccagcacg tccacggaaa cccgaagctc
    23761 cttgagcctc tccttgtgct tgactccgaa ccggtgttcc tcgtcgatga tgaccaggcc
    23821 caggtcccgg aatgccacgt ccttctggag cagtcggtgg gtcccgatga cgatgtccac
    23881 cgttcccccg gccagtccct cgaggacccg cttctgctgc gctgcggttt tgaagcggct
    23941 gagtccggcc acctcgatgg gaaaaccctg aaagcgctcg gtaaacgtct ggtaatgctg
    24001 ctcggccagg accgtggtgg ggaccagcat ggccacctgc ttgttgtcca tgaccgcccg
    24061 gaaggccgcc ctgagggcca cctccgtctt tccgtacccc gcgtcgccgc agaccagccg
    24121 gtccatgggc cgccgggaac tcatgtcctc gagaacgtct ccaatggccc tcatctggtc
    24181 cggggtttcc tcgaaggcga aagtggcctc gaattcccgg aggtacttgt ccggcccgga
    24241 aaaggcgaac ccttcccgga cccggcggac ggcatacagt tgcaggagtt ccgccgcaat
    24301 gcgttgtgcg gactcgaggg ccttcttctt ggccgtctcc cagctcttgc cccccagttt
    24361 gtccaccttg ggttcgtggc cctcgatgcc catatacttc tggacccgct ggagcttgtc
    24421 cacgggaacg tagagacggt cgccgtcctg gtattcgagg agcaggaagt cgctctccag
    24481 gccccgaacg gaaaggtgaa ccagttcccg gtagatgccc accccgtgat ccacgtgaac
    24541 cacgaaatcg ttggcatgaa ggtcctggaa ggaatggaga aacaccccca aaggcggttt
    24601 ggatgcccgg cggcagcctc gtttctcgaa gatctcctct tcgctcacaa cggccagccg
    24661 ctcccggggc cagtagaaac ccttctcgag ggcgcccacc aggaccttga cggcaggggc
    24721 gtcgaaggat tcctccccga agggcaggga gctgaaaagg gcgtccaccc cgtagcttct
    24781 caaaaggtcg gtgagcctgc gggcctgttc cgcgtgacgg caaaaaaggg aaggacggat
    24841 tccttgggat tgccattcct ggaactttcg ggccaggggt tccaggagcc tttcccgggt
    24901 tgggtgagat ttgatggaaa tcttgagttc ttcatggccc cgggtgccca ggtcgaaaac
    24961 cttggtgtcc gggccgtcgg aaagggaatt cacccgaagt tgctggaagg actcgatcct
    25021 tgtgagcaag tcttcggggt ctccaaagag ccgggaggga gggatcttcc attcgtgggg
    25081 agtctcccgg gtctcaccct cggagaggac ctcccagtag agtttgtcca tgcgttcccg
    25141 gacccccgcg gcatcggacc acaccacgag gcatcgcttt tcgagatagt cgaggagcgt
    25201 caccatcttt tcgtgaaaga cgggtagaat ggcctcgtag gctcccagct ggtgaccttc
    25261 ctggaatttc tcgagccaga cgttgccggc ggccggggtc agcctttccg cctggacgtc
    25321 gtggtagacg gccttctgag cccgttccag ggcttcggcg ttgaggacga cttcgcttgc
    25381 gggcaggagc agggcctcct cgatggtccc catagagcgt tgattggctg ggttaaacag
    25441 gcgaacggac tcgatttcgt cgtcgaagaa ctccagcctc agggggtacg ggtagagggg
    25501 tgcatacaca tccaggattc cccctcgtcg accgtagtct ccgtattctt ccaccagggg
    25561 ggttcgataa taacccttct cgtccaggcg gcggcacagg gcctcggggt ccagccgatc
    25621 ccccttgacc aggtactcgg cgttctgggt gaggatctcc ggagggggca gcctctcgag
    25681 gaggcttcgg gccgaagcgg ccaccaggat gggggaagaa cccgcacgaa gggcatacaa
    25741 ggcctcgatc ctccgggccg tggcctccat ctttcccagc cactgggctt tgtgcccgct
    25801 tcgggacgga agcacccata cctgtcggtc caggggagat tcggggctga aacggttctt
    25861 tcccagaaac aaggtgacgg aatcggcgag cttctgggcc tcggcgtcgg tggcggcgac
    25921 caggagcacg ggctttcgac ccgagcgcaa ctggagggcc aggaggtagg ccagggcccc
    25981 gggccggact ccccgaaggg agagggattc cccggaggcc tcgatctcct gcaggattcc
    26041 ccgggaatcg ctctgggaaa gaaaggactc gatgggggtc gacgattcgg gcaggccctg
    26101 cacaacggtt ggggagtcca tgattcgagt cttggcatct tccgtttcaa aaagttcttc
    26161 gctccaaaac gaagtttcca ctataaccaa cgtagccacg gaaaggaagt tcccgtataa
    26221 taaaaggccc cgcggaccat cggcggcaat cactcctaac ccaaggcaac atgaggcccg
    26281 gcatgacgga accgaccctg cagtccttgc tgcgaaagct gcccagcgta gatgccctcc
    26341 tccacatgga ttccatccga gaggccctct cgaatcaccc gcgaaaactg gtgatcgaaa
    26401 gcctccggta tgccattgaa acccagagac agcggatcct ccgggacccg gagtccccgg
    26461 accgggtgga tgccagcccc cggttcgtgg cccggctggc cctggaacac ctgcagtctt
    26521 ccagcgacta taccctccga agggtggtca acgccacggg cgtcatcgtt cacaccaacc
    26581 tggggcgatc tctcctgccc caagaggcgg tggatcgcct ggtcaccttg tgtcgcagct
    26641 acaacaatct cgaatacgac ctggagcggg gacagcgggg ctcccgctac gttcatgccg
    26701 aagccgtttt gtgtgaactt accggcgcag aggcggcgct ggtggtcaac aacaacgccg
    26761 gggccgtctt cctggtcctg aacaccctgg cccgggaccg ggaggtcgtc gtgtcccggg
    26821 ggcaactggt ggaaatcggg ggctctttcc ggattcccga cgtcatgcga agcagcgggg
    26881 ccgcgttgaa agatgtaggg tgcaccaacc gcacccatct gcgggactac gagggcgcca
    26941 tcaacgagcg gacggcggcc ctcatgaagg tccacaccag caattaccgc atcatcgggt
    27001 tcacggcgga ggtgaccctg gaagaactgg tgaccttggg acggcaatac cggttgcccg
    27061 tgatcgagga cctggggagc gggtccttcg tcgatctttc ccggtttgga ctcctggggg
    27121 aacccaccgt gcaggaggcg gtcaaggcgg gggccgacgt ggtgacgttc agcggggaca
    27181 aactcctggg aggaccccag gcggggatca tcgtgggacg caaggacctt ctcgatcagt
    27241 gccgcaaaaa ccccctcacc cgcgccttgc gggtggacaa gatgaccctg gcggccctcg
    27301 aggccaccct tcgcctgtac cgggacgaag cccgcgccat ggaggccgtt cccaccctgg
    27361 ggatgatcgc cctatccccg acggtcctcg aggaacgggc ccagggcctg gcggcgctcc
    27421 ttcgggaggc ggactcccgg ggggttctcg aaatcaaggt caggccctgc ttttcccagg
    27481 tgggaggcgg gtcccttccc ggacagaacc tggccggtca cggggtggcg gtgagttcga
    27541 cggtcctgag tcccaaccga atggaaatgc tgttgcgggc caacaaaccc cccatcatcg
    27601 ggcgcatcga atccgagcaa tacctcatgg acgtgcgcac cattcagccc gacgagtggc
    27661 ccatcatccg ggaagcgttc gtcaggatgc tcgaaagagc cgaaggccgg gaagaaacct
    27721 cctgagagca ggggggaggg ggcgcggagc gtctttacgc gcccggcccg aatccatttc
    27781 ctcggtgact tcccggacct gagttctctg cggggaagac gacgcgggag accggggacc
    27841 gggctctttc atcctcgagg gaaaatgcgg actcagggga ccacccgcaa ttgcaggtag
    27901 agatcgcctc ttcgtccgga ggtccgggtg gagtgaagga gccccatttc cttgaggcga
    27961 aggcgggtgc cgggcttcac cccgggggga acgacgacgg aaaccttttt tccgcggccc
    28021 aggtgcggca cttcgatctc gacggtggtc cccatgaagg cttcgtcggg ggagattttc
    28081 aggctgtagg tgacgtcgga tcctcgcctc cgcccgggtt ttgccccact tcccaccgcc
    28141 cccgaaacca cttccgacgc cttcttccag atggactttg ccagcccctt tccggcctgg
    28201 gccaaaagag aaatcccccg ggagacgatc cccggggatt ccgcaggcgt ggctggcgga
    28261 ggagaaggtc tgcgggcggc ctgccccggt ggttgggttc cccgggacgt atagcggaag
    28321 acgcgggtct ggccgggtcc gccccagaag atgctctgaa agaagatggt cttgtctccg
    28381 aagaagatcc ggttgacgaa ccgttcatcg aagcggaatc ccatccgttc gaattccttc
    28441 tgaaggtccc agaacgcatc ctgtccctgg cggctgctga aaaagtcccg gaagatttcc
    28501 tcctgggagt agccgaaccc ggtacgggag gtgcccccgg agggccggtg cgtgccgaac
    28561 cggcggtatt cgtcgtactg ggcgcgtttc cgggcatcca tgaggacccc gtaggcctcg
    28621 ctgatcttct tgaaccgctc ttcggcccga gcgtcgttgg ggttacggtc cgggtgggtt
    28681 tccagggcct gtttgcggta ggccttcttg atatcctcga tggaggcgtc cggggaaacc
    28741 cccaggatct ggtagtaatc gtcgtgggac attgggtccg actctcctca tctggccccg
    28801 aaagcgggtt aaaagacgtc cgggaatacc ctcgttgtga ccctccaagt ataaagtccc
    28861 gaattgccaa gatcaagaag agcctgagtt cgaaggcccc cgggccggga accctccgaa
    28921 gcctgcaggg ccggggcgga ccctcccggg gaatcggttt gcgttaagga aggaagatca
    28981 cctggcgctt gtcggttcct tccaccgggt aaacccggac ccgggtttgg tagagctcct
    29041 cgaggatttc gggggtgagc accgaggcgg tgggaccgtc ggccgccagg tttccttcct
    29101 tgagaaagat caagcggtcg cagtagaggg cggccaggtt cacatcgtgc aacacggcca
    29161 ggacggtgag ttgtgtttcg cggttgagcc tctcgagaat tccaaagatc tggagcttcc
    29221 gatggatgtc catggccgag gtggcttcgt ccaggaaaag gaggggggtg tcctgggcca
    29281 gggccttggc catggtgacc cgctgttttt cccccccgct caccgccgtg atgagccgcg
    29341 gggccagtgg cgtggtttcg gtgatttcca aggcccggtc caccacggct gcatcctcgg
    29401 gactgtccat gcgccaccgt ttctggtgcg gatagcgccc catgtgcacc acttcctcgc
    29461 acgagaaggg aaagcgcacg tcggcatcct gggaaaccgc cgccatcttc ctggcccggc
    29521 tcttgggtcc cagggttgcc agggattctc cgaagatctc caccgacccc gaggaagggg
    29581 aaaggatgcc ggtgagcgcc aagaggagag tggtcttgcc ggaaccgttg gggcccagga
    29641 ttcccacgaa ttccccccgg tgaatcccaa aggagattcc cttgaggacg ggatcgggtc
    29701 cgtagccgca ccagaggtcc gaaacacgga ctacagttgc aggtattttt tccggtgcat
    29761 cagcaggaag caaaagaagg gtcctcccag aatggccgtc accactccca ccgggatctc
    29821 ttccccattg ggaagggcgc ttcgggccag ggtgtccgaa agaagcacca ggaggcctcc
    29881 tcccagcagg acggcgggca gcagcttccg gtgccggggc ccgatggcca gtcggaccag
    29941 gtgaggggtc acaaggccca caaaaccgat gatgccgctt accgccacgg cggctgcggt
    30001 gatcaacgag gcggccagga gcaggtgaaa ccgaacccgg gcgacgtcta ctcccagttg
    30061 ctgtgcctgg atatccccca ggctgaggat atcgagttcc cgggcataat agagaatggc
    30121 ggtccctcct aggatcaggt atggcaaaac cacggccacc tgggtccagc tctttcccga
    30181 aaagcttccc aggatccaaa aaacgatggt ggaaacggaa tcttcgttca ggctcttgag
    30241 caggctgatc catgccgaaa gaaaggtcga gacgacaatg cccgccagga ccagggtggt
    30301 ggcatgaagc cttccgtcca ttctccccag catgtagacc aggtacaggg cccccagtgc
    30361 ccctgcgaag gccgctgcgg gtaaagggtt gacgttcccg agaagagtcc ctccccagcc
    30421 caaaatgatg cagagggaag cgccgaaggc ggcacccgcc gagatcccca gggtgaacgg
    30481 gtccgccagg ggattcaaga gcactccctg gtacaccgcc ccggccaggg ccagggcgct
    30541 gcccacgacc agggccaggc aaacccgggc cagccgtaca tggacggcga tatagcgggt
    30601 cgtatcgtcg atgccttgag cggcggaggg atccaggggg gaaatccaga ttcgccagat
    30661 ggtttcgtag gggatgggaa agggccccat gccggaggtt acgaaggcgg caagggcaat
    30721 gaggaggcag agcccgatcc agccgagcca acggggcaga gaatgggaca acggtgacgt
    30781 caaggaaacc gcctcgggag cacccggatc cgtcagggtt ccaggcctcg tttcttgatg
    30841 atcatgagag agaggtagtg aggttgttga atctcgcaaa cccgcttgaa gtcccgctcg
    30901 accacctgcc cgtccaatcc gcagcggctc acgaaacagc agctgtcggt cagcccggct
    30961 tcctcgatct gggaaacaat ctgcgggaat tgcttgtagg tcttcatgag aaccacattc
    31021 tccgataccc gaatggcctc tttgagtcga tccccgccct tggctcccga aatgatggtg
    31081 atggcttcct cgccttcagc cagaggaacc gtggcacagg cagctgcggc ctgaaaggag
    31141 gtgattccgg gaacggtgac gatcttcacc ccgggaagcc ggttgcggat ctttcgcatc
    31201 aggtaaatga aggtgctgaa ggtgaggggg tccccgatgg tcaggaaggc cacatgtttg
    31261 ccttccccga ggatttgaac cacacgttcg cagttggccg tccatgcgtt ttcgaggctc
    31321 tccgcgtgga aggtcatggg aaacagcaga tggtccacgg gggtctcggg gggaaggtgc
    31381 tcccgcacga tttccagggc caggctgtaa tcgttcttgc tcgatgcggc ggcaaagacg
    31441 tgggagaccg ccttgagaac ccgtaccgcc ttgagggtca ggagctccgg atctccgggt
    31501 ccgacgccga tcccgtacaa tgtacccagc atggcttgtt cctcattcac cggcttcctc
    31561 ctcgcgagca tgccgtgcca gctccaggag agcgtggagg acggctacgg ccaccgtgct
    31621 cccgcctttt ctgcccaaaa cggtgatgtt cggctggtcg ggaagatccc agagggcctc
    31681 cttggattcg tccgcccgga caaaccccac ggggaccccg atgaccaacc ccggcctcaa
    31741 cccctcttcc cggatgagcc ggacgacctc caacaaggcc gtgggagcgt tgcccacggt
    31801 gacaatgttc ccgtccatgc ggcgggaaac ataccggaac gccgccacgg accgcgtggt
    31861 gcccatggcc tgcccccact cgtgactatc gggatgcacc gcgggaatga cgacctcgtt
    31921 gccgaaccat cccaggcgcc agggggaaag tccggcgtga atcatgcggg tgtccacaaa
    31981 gacggaggcc cccgcccgga gcgcttggat ccccgcatcc acggcccggg gatggaatcg
    32041 aaaccaacgg gcgaaatcga aatccccggt ggcgtgtatg acccggcgca ccacctccca
    32101 ctggggaggc gaaaaggaat ggtccggcat ctcccggtcg atgatgcgaa agctttcgac
    32161 ctcgatgcgc cgtccggctt ccaccaggcc cgggcggtcc gttttcaatg attccgatga
    32221 atgactcacg ttcttgtgtc ccaaaaatgt gaaaagcccc cgcagacggg ggtcccactg
    32281 ccccaaggtt gtcgtcgaat gccccggcct cactccaagg ggctgccccg gctgggggcc
    32341 aaaaacaaaa aatccctgaa ggtcctgcat cggaccttca aggattgcac ccagcttcac
    32401 ttgatccttt cacttctcgg ggagatccct cgttccccgg ggccgttcga ggttgaggcc
    32461 cccgaccgtt ccacggggag agatcctccc ccaaaaccat ggtcgggcgc cctcgaagcc
    32521 gcccttcccg ttcaggcagg ttttctgact cacggatcgt tctactcacc atcccttccc
    32581 ggcgccctcc gtgggcagcc agtggtcatg atggatttcg tccccgttca cagcggcggg
    32641 accgcgacgg actttcaccg tcttccctct tgaatctcac ccatgcgaga atacctgaac
    32701 gcgcatcttg tcaaagaacc cgcccccccg gggaagcgag accggccagg accccgcctg
    32761 caacaagaag gaatccctcc ccggaaggta tcccgattct taccccattt gatccgcccc
    32821 gtcaacggga atgcagaacg ctccgccggg gcgcccccgg cggtgggcga cggccccggg
    32881 actgtggaag ggaggcgggt ccaacaaagt ctttgacccg gccgggggca ttgtgattca
    32941 atctcaaccc tggctccgga cgatgacttc aataaggaaa ttccgaaagt tatcatggat
    33001 tctaggaggg tggtgcaacc gtgcttcggg tgcctccggc aagagaggag tgatgcatgt
    33061 cttcttggaa acgatggtta tggccgctgg cgggcggttt catcggattg gggaccctgg
    33121 gatttgccgt gagttttacc tcggcctcct acctggtgga cctctggtgg tttcgatccc
    33181 tgggctatga attctactac tggcagaggc tcttgtatcg ctacgtgatc ttcggggtgg
    33241 tgaccctgct gtttttcctg atcttttttc tcaacttccg ggtggcttcc cggcacctgg
    33301 gctcgacggt tcctccgacc tccggggcgc ggccgtcctc gatccgggcc tactcggatc
    33361 ttctgagact gttccgcacg ggctccatgt gggtctatac ccccctgtcc ctcatacttg
    33421 ccgttcccat cgctctgccc ctctttcagc gatgggagga cttcctcctt ttcgtattcg
    33481 ctccctccgc cggtctgcag gaccccgtat tcggaaaaga cgtgggattt tacctgtttt
    33541 cccttcccat ctactcgctt ctccagaagc ggttgttcct ggccttcctt ctcctgctcc
    33601 acggtttgct cctcctctac tggctcgaga gacgactgct ggtgcggcgg gacgagcgga
    33661 tgcctcgagg agccaagtgg cacatcaaca gcctggttct tctgaccacg ctcctggggg
    33721 tgggcgtctt tgcccttcaa cgctacgagc tcctgtatgc cgaaactcac gagcctctct
    33781 ttttcgggcc gggctacgaa gagatgaagg tgattttgcc catgatctgg atggtcatgg
    33841 tcctgctcct ggccacggcc gccctgctgg tgcatttcat ctacacccgg aaagactgga
    33901 ggcccctggc cggggctgcc gcctgcctgt gcctggtcct gtggggccgg tactcggatt
    33961 ttcttcccag cgtggtcaac cgctacattg tgaagcccaa cgccctgtct ctggaaaagc
    34021 cctacatcgc caacagcgtt tcggccactc ttgccgccta cgatctggat cgagtcgaag
    34081 tccgggactt ctccccggaa cggatgcctc ggggcgtgcc cgagcccaag gtccaggaca
    34141 tcctgcgaaa cgttcccgtc tgggacggtg agctgctcac caacgtgtat gaacagctcc
    34201 aggagctgag gacctactac gatttctcgg gagtggatgt gagcctctac gaagtccggg
    34261 ggaacctgca gcaggtgttt ttggcggcga gggagctcaa tgccgacaag ttgccctcgg
    34321 gggcccgcaa ctgggtcaac gagcacctca cctacaccca cgggtacggt gtggtcatga
    34381 cccctgcggc ccaggggggg gacgagccca tcacctggtt cattcgggga attccggtgg
    34441 aatcggagtt cggcttcaac attcggcagc cgggcatcta ttacggggaa ctggatcact
    34501 accg
//
LOCUS       DAOOVC010000027        34317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_836_length_34317_cov_7.248322, whole genome shotgun sequence.
ACCESSION   DAOOVC010000027 DAOOVC010000000
VERSION     DAOOVC010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 34317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34317
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_34317_cov_7.248322"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..696
                     /locus_tag="PLM79_05635"
     CDS             424..696
                     /locus_tag="PLM79_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85822.1"
                     /translation="MLFSIQAILGATFLYAGWLTLDRHLTGGVVLMALGLACSVGPIL
                     QGLAFARRVLPVSPPRSPQKRKSPKIIELQRHRSHGEQEKNRPTIH"
     gene            782..1474
                     /locus_tag="PLM79_05640"
     CDS             782..1474
                     /locus_tag="PLM79_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HOV85823.1"
                     /translation="MLPKTLILFLAVWSAGTAVPCGTIAGTEEISRVQSAAAVLSEIM
                     EIPEDCVPPSLLNNSRAVAIIPGMLKAGFVIGGRYGEGVLMVRNSRGRWGNPLFVRIY
                     GGSVGWQVGAQATDVILVFKTMRGVDAVTSGKFTLGLDASVAAGPVGRHAEAGTDVQL
                     RAEILSYSRSRGLFAGVALEGAAIQIDYGANAAFYALAGLLPAQIIESEDLEAPSVAW
                     DLRQVLTRYADS"
     gene            1767..2600
                     /locus_tag="PLM79_05645"
     CDS             1767..2600
                     /locus_tag="PLM79_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOV85824.1"
                     /translation="MRVSCRTKGFLRQYFFGFAALAAAVILWVGVPQSYGQSADSSKK
                     QLELNSNLFAWAKKGDVQKVNAWISQGADVNGRDSDGMTPLMHAALRGHKEVVLSLLH
                     GGAEVDLSDGNGVTALMQASWAGNVEILRILLSNGADLNLRNTDKWPSLKKAGATPLM
                     AAAMNGNLDTAAILIANGASLDEQDDDGQTALVMATRGGHVQMVKLLIRNKANTELRD
                     KYGRTALTIAVQQGDSRVAMALLKGGADREASDINHWNAQQYAWCLGREEILRAMNRQ
                     P"
     gene            3175..3945
                     /locus_tag="PLM79_05650"
     CDS             3175..3945
                     /locus_tag="PLM79_05650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOV85825.1"
                     /translation="MPNDGGIVKRSRASSPQPHEAPASEMNAHGCSKPDLPPTCPLGV
                     QDCPVRSTVEELQEECRRLTELSHTDPLTGLFNLRYLREAMDQEMERTRRTGLATGLI
                     MIDLDHFKLVNDIHGHQCGNEALRWTARTLRRNIRRLDIPCRYGGEEFAIVLPATRLP
                     HAIRLAERLRLAVMDSPLHYEGIWVPLRASFGVDIYRPREPGSVTVEDFIRRTDEFLL
                     QAKTLGRNRVCCRQGDGSRPATETTAQERAALFITRWP"
     gene            4096..4869
                     /locus_tag="PLM79_05655"
     CDS             4096..4869
                     /locus_tag="PLM79_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOV85826.1"
                     /translation="MDRRNSPVTICVSSGKGGVGKTSFTVNMALALARKQQRVLVIDA
                     DLGLANVDVLFGLHVERTIRDLLEDGSDPMESVLYLEPNLGVLPASSGVPEMVNLDPD
                     DQLRMEGILQDISRHFDYVFLDTAAGIGPSVLWFNHFADHNFIVLSPDPTSITDAYAL
                     IKVLSRDYQREDFFLILNLVTNEGEGRRTYDNVARVVKQFLKLSPQYFGAIPVDEAVV
                     KANRERIPFLKLSPQSKAAQAIVRMADQVLKLQKACSPT"
     gene            complement(4933..5421)
                     /locus_tag="PLM79_05660"
     CDS             complement(4933..5421)
                     /locus_tag="PLM79_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85827.1"
                     /translation="MVWVALLGGFAGVLLAFSATRRKGDFRKKLLEDLDHHRSQGTMG
                     AFVNAILRDRNSWLKPLCWIRKPFSREVNLAVETAALLLGIMALVTSMTSFDKAFKTP
                     VEGMAVIFVLGAVLAYIPGEWFFSRRIDAEIEAVWKEMEEARDGKRLDGFLEQATKRW
                     GP"
     gene            5807..>7243
                     /locus_tag="PLM79_05665"
     CDS             5807..>7243
                     /locus_tag="PLM79_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOV85828.1"
                     /translation="MESLSVKNLCIYHTLDALTEGLSHFSGSSRTALLYAEKREDPMR
                     VYDPQDLLAGHEPKLKELYLDSDLWRSRIPFFENNQLFGQILPERKLPLTGLITYGGK
                     SRSIFYQMWFAEQHPDMCSTGPTERWLEHAACLLAHDFAHEEAFYTGSARYVLREYST
                     HAVRDFIWDELNRRFGWDIWMEVYTILDSILAISKTPEEGAWPRGTLVFVEPSALKKL
                     DFMVRFPVVERLGLRNYKHVRKLLLAVEDSDRKLISDGQQVVGIMTGELPMCRITADF
                     RGGHGFLRLMGEPVCSFFDGRYHSTNRKPKLVQLEELLLESPADQSTSHILFKTVAAI
                     VEKAGERKHGCTVVIDFNDPPLSIAGQKLEQPIDLQQEASLELAKSLAKVDGALHLGG
                     DLRLHSFACLLDGRAVPGEDRSRGARYNSALRFTAERKNLIVVVVSSDRPVSVIQGGV
                     EITASSEWAPFAQLMTRPPTLEEWLARSC"
     assembly_gap    7244..7343
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7344..8375)
                     /locus_tag="PLM79_05670"
     CDS             complement(7344..8375)
                     /locus_tag="PLM79_05670"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HOV85829.1"
                     /translation="MLHVSSLGRIQEPPGWEAAPSDKKPEDSFSRTLSSEVPGGTDLA
                     GVLDRILKDPAGQSSKPPMKFGILQSTLREYDPRGTVAASVKELNEEKAARIYTKIWE
                     RAGCDLLSRPLNVLHFETFLQDPRKAMDLLTRSRGDVRAYESLRAEAGGWESGAPAAE
                     GPSEKLACPTGAAYPPPSCIACDSVSNAVAARRIASYGNASKKDFETAVAFVIDQEGR
                     QVVPNDNGKGPSRFGILQTTLRRMDPRHKIARSVGQLDERRARQIYRKIWEDSGCDKL
                     PYPINILHFDTTVHRPQTAVKALRASGGDPAAYLKARQDSLRTLKSYPRFARNWEERM
                     RSLSELIKG"
     gene            complement(8722..9591)
                     /locus_tag="PLM79_05675"
     CDS             complement(8722..9591)
                     /locus_tag="PLM79_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase"
                     /protein_id="HOV85830.1"
                     /translation="MKKTTLFRKLIQDPEILLLPCCHDGLSARVLEQSGFKAIAAAGY
                     GLSGSLLGMPDIGVLTASEMITQYGHICSAVNVPVLVDLDTGFGDVNNVIRAVRDCEK
                     AGVAGLFLEDQTYPKRCGHMAGKSVVSVEEYLPKLKAALWAREDPDLVIMARTDSCAV
                     YGIEEAVRRARIYAEAGADMIFVEAVHTTEDMRKVNSALAESNTPSMANMIEGGRTPF
                     LSAGELQELGYSVVAYPCGSVFAAVKALQRWAGYLKEHGTTRGYRDQMLSFDEYFEFI
                     GVPAVREREKRFR"
     gene            9926..10855
                     /locus_tag="PLM79_05680"
     CDS             9926..10855
                     /locus_tag="PLM79_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV85831.1"
                     /translation="MRSGTLMKMLLGILFLCPGFLLFSYASVLRVHGAEVLRVTDDSG
                     QTVELPQPARRVIPLYGAFAEMLYAIGAGDSVAARTQADRFPPDLLRLPSVGTHMKPN
                     VEMILGLKPDLVIQSSSRREAIPEIRRIQAAGIPLVVFDPKSFPQIFSTMIKMGVLTG
                     KEGSAREAVEQLEGRLKKAGIRLEGSTRELRVFYEVRGEPLTAAGQGSIIQEILVAAG
                     AKNVVSSPKALVQYSVEALLVDDPDVYIVQRGPMNRNPAPPDRRQHFSRLRCVREGRV
                     LFVDEFIFSRPGPRCVDAVEQLAEKLRPYGKSS"
     gene            10839..12155
                     /gene="cbiD"
                     /locus_tag="PLM79_05685"
     CDS             10839..12155
                     /gene="cbiD"
                     /locus_tag="PLM79_05685"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: protein motif:HMM:NF014004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HOV85832.1"
                     /translation="MESHPENDMKSTDRPVSKRRKPKKHRLRMGFSTGTAVTAAAQAA
                     MRHLLTGESPRAISVRLPAGYYLPIPVEETQRNGTSAGARVIKDGGDDPDVTHKAEVR
                     ARVTCLRSTGTDPTDAAVVSEQSSPGVWLLGGEGVGLVTKAGLPVPIGEPAVNPGPRQ
                     MLLAGLTRELLRVDADLITRVVSDERIPNSSHLSDGRVFLPFLEEDSRLAGLSVCLEI
                     SVPRGKELARHTLNPRLGIVGGISILGTTGLVKPFSHQAYEETIRVSLAVAHSSRCRT
                     VVLSTGGKSERYAQDALPFLPPEAFVQIADFFAFAVKEAAAAGFEEIVHSVFFGKAVK
                     MAQGHAYTHAHKVSMDLLPLARLALDRGYGAGFSRQIEEANTARHALEILRQEQAWDL
                     VQMVAVQARDRSRKACRPGTRVRLLLFDYDGSLLADIRDEGDKGWA"
     gene            12146..13426
                     /gene="cbiE"
                     /locus_tag="PLM79_05690"
     CDS             12146..13426
                     /gene="cbiE"
                     /locus_tag="PLM79_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HOV85833.1"
                     /translation="MGITPEGWEPPLVVLIGMGTDREDLSLRALRWIEQAEVLAGGRR
                     HLAGFPKHPGEKIPLESPLGGALEKIQRVSRERKTAVLASGDPFFFGIGPLLARQVGE
                     ERLLTLPNVTSPQVLFSRAARPWDRLQTVSFHGRGSDPLDRDWLRLVGSRMPVACFTD
                     EDHSPAWIARQLVEAGQGHCTLLVGEDLGSIRERILRLSPEEALGREYSPLNLVAVLP
                     GEEGGPADPHGVEKEEGEAAVFGLAEEAFRHRAGMITKSEIRAVVLAQLRLEPGLTLW
                     DLGAGSGSVAIEASRAVALGEVVAVEKDAARFADLLHNVRRLGRGNIRAVHGDVGALV
                     DGLPDPDRVFIGGGGKSMESILSKILGKLRPRGRVVQNVVTLESLSRASSFWQSASRE
                     FSVTQVQVNRSVPLGNTFRFEPLNPVFVITAWAK"
     gene            13434..14216
                     /gene="cobM"
                     /locus_tag="PLM79_05695"
     CDS             13434..14216
                     /gene="cobM"
                     /locus_tag="PLM79_05695"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HOV85834.1"
                     /translation="MKHPVAFVGAGPGDPELITVKGRRLLREADCIVYAGSLVPESLL
                     GWARPEARRHNSASLTLEETHALLKEACLAGDRVVRLHTGDPSLYGAIREQMALLEKD
                     GIPYEVVPGVSAVFAAAASLRKELTLPGISQTVILTRLSGRTPVPEPEALRSLATHRA
                     TMAVYLSVQEMETVVSELAFSYPPETPVVVAYRVGWPDELLLRGTLEDIASRVKASGI
                     RRQALILIGGVLDPPDDPEAPLRSRLYDETFSHGYREAKDRV"
     gene            14185..15291
                     /locus_tag="PLM79_05700"
     CDS             14185..15291
                     /locus_tag="PLM79_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HOV85835.1"
                     /translation="MDIEKPRTGSESPTAIVALTRHAVELALRLQPELPSSVCYVPVR
                     HRFALAMGARGFQRLGGLLGEIWPRYSAFVFIMAAGIAVRRIAPLIKSKLSDPAVVVL
                     DERGQFVVSLLSGHLGGANQLALKVARLLGAQPVITTASDVWKKPAMDLVAQEAGLEV
                     ENPEMLARLTRAFLEDEEVWVFDPEGRIEDSLGDYPHVRRLSPGEPETVRERPGDPGA
                     PPPGLWVHETVPSPEGIRFLLLRPQNLVVGIGCNRGTPMEEMRDFLESVFREHRLSLR
                     SVRNLASVDLKSREEGMIKLAETLRRPIRFYSREELRNVSVPNPSLRVANHIGVHSVC
                     EAAAILSAQSPTLLVPKQKTPNVTLAVARADCPS"
     gene            15288..16139
                     /gene="cobJ"
                     /locus_tag="PLM79_05705"
     CDS             15288..16139
                     /gene="cobJ"
                     /locus_tag="PLM79_05705"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: protein motif:HMM:TIGR01466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HOV85836.1"
                     /translation="MSLGPGDRSLLVAEALEALREAEVIVGYTTYLELIPDLTPGKKV
                     LGSGMRKEIPRCRAAIEHARNGHRVALVSSGDAGIYGMAGLVLDLCRDSGLRVCSVRE
                     KPPETCDLQFEVIPGVAAFNAAASLVGAPLMHDFAAVSLSDHLTPWELIEKRLQAVAA
                     ADFVTAVYNPRSKARPDLLEKACEILLEHRSPETPVAIVRRAKRQGEWVHLTTLDRIP
                     FDEVDMQSILLIGNSRTYLWDRWMVTPRGYLDKYGEGAEGEGREAGSERLRARRLDHC
                     GPDAESG"
     gene            complement(16411..17277)
                     /locus_tag="PLM79_05710"
     CDS             complement(16411..17277)
                     /locus_tag="PLM79_05710"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV85837.1"
                     /translation="MEPEKTPAESTLLDRIVRTVGDLPTMPAVANMVLDKLADPNATA
                     KQLHQLISQDQALAARVLRIANSPFYGCAKNITRLTDAIVLMGFNSIRSLVMTSVLQD
                     FFKNFGLAEKLLWEHSIGCAAASKRVAALLRYPKTEEAFLAGLLHDIGKVVLNLKLPD
                     QMLEIVQEVYNSPGKTGFRAVELRTLGFDHAQIGEQIARKWNFAKEIEETIGNHHHPQ
                     RAKVAPVVCCIVHLANALCHKLEIGPTRNPDLDMKTVVSARALKLTPEAVEKLLEEVS
                     QVMASQQDTFFS"
     gene            complement(17606..17902)
                     /locus_tag="PLM79_05715"
     CDS             complement(17606..17902)
                     /locus_tag="PLM79_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85838.1"
                     /translation="MKLFKLFTTGLLLAIVALFFWQNVSAFQTPVPFQLNLYLRQPLN
                     WTLSLCTLLFSSLVLGFIVGFGLMLKPYFRARRLAVEGKKTKEVEAGSEADRAQ"
     gene            complement(17889..18389)
                     /locus_tag="PLM79_05720"
     CDS             complement(17889..18389)
                     /locus_tag="PLM79_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOV85839.1"
                     /translation="MENLWAPWRIEYILGKREPYCIFCPEGHGVSDEERLILHRGRLC
                     MVMMNKYPYNNGHLLIAPWRHVAALDEITEEETLSIMKWLQKSIFILKSVMRPNGFNV
                     GLNLGSAAGAGVEDHLHFHLVPRWQGDTNFLTVFAELRSIPEHLKQTYAKLLPKFKKE
                     SADEAL"
     gene            complement(18395..18895)
                     /locus_tag="PLM79_05725"
     CDS             complement(18395..18895)
                     /locus_tag="PLM79_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV85840.1"
                     /translation="MRARAAMKIVVMSDTHLSRPTDHFRALCDCYGKDADLVIHLGDL
                     VSTGILDYLEQYPLEAVAGNMDPAAIQERLPPTKVLRLGKFRVGLAHGWGSHHAIRAR
                     LAHHFPTVDAILFGHTHQPMLLEEDGIFWFNPGSVSLGRGALSHSLGILHVNEKLTGE
                     IIALQE"
     gene            complement(19195..19938)
                     /locus_tag="PLM79_05730"
     CDS             complement(19195..19938)
                     /locus_tag="PLM79_05730"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HOV85841.1"
                     /translation="MKKPYTNPRRFYRVQHSKRQGWVTFQVRHKETDLWIRAQRSLKQ
                     EALTCVLQCRHQLDEYIAARPEFLKSLVPIPDDLTAPPPARRMIEAARAAGVGPMAGV
                     AGAVAEATALSLKPLSPGIVVENGGDCYMDLAEETTVGIFAGPHSPFTGRLGLRFTAD
                     RFPLGICTSSATVGHSLSLGRADAVTVVSRDVVLADCAATALGNRVKTSADLEKTLEE
                     AAEIASIEGVLILIKDRMAVWGDLELLPL"
     gene            complement(19990..21546)
                     /gene="guaA"
                     /locus_tag="PLM79_05735"
     CDS             complement(19990..21546)
                     /gene="guaA"
                     /locus_tag="PLM79_05735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOV85842.1"
                     /translation="MTIDPLHEDRVLILDFGSQYTQLIARRVRESHVYCEIHPFNLSL
                     EKIRAFGPKGILLSGGPAGVYDLNAPFSDPGLFGLGIPVLGICYGMQLMARQLGGAVE
                     RADRREYGHALVEVQDPEKLFRDIEPEGVRVWMSHGDRILELPPGFRVLASSSNSPIA
                     AMADPESRFYGVQFHPEVVHTPCGKTLLENFLYGICRCRPTWTMKSFVESVTRSLVEK
                     IGSDHVICALSGGVDSSVVAVLLHNAIGSQLRCVFVDNGVLRKGEAESVRRVFRDHFE
                     MNLTYLDAGHLFLDRLRGVTDPEAKRKIIGNLFIELFEKEARRLPPAKYLAQGTLYPD
                     VIESVSFKGPSATIKTHHNVGGLPDRMDLELIEPLRELFKDEVRLVGRELGLPERIIL
                     RHPFPGPGLAIRILGEVTSEKLEILREADHIILEEMELAQWNDRVWQAFGVLLPVRSV
                     GVMGDERTYEQALVLRAVESVDAMTADWAKLPYDLLQRISNRIINEVKGINRVVYDIS
                     SKPPSTIEWE"
     gene            complement(21588..23066)
                     /gene="guaB"
                     /locus_tag="PLM79_05740"
     CDS             complement(21588..23066)
                     /gene="guaB"
                     /locus_tag="PLM79_05740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOV85843.1"
                     /translation="MNEKYADHVPEALTFDDISLLPQYSEVLPNDTSVETRITRSIPM
                     RIPIASAAMDTVTEADLAISIAREGGIGIVHRNMPIERQAQEVSKVKKSESGMVVDPV
                     TVEPDQRISDVLDLMSRYHISGVPVVKAGQLVGIITNRDLRFETNLDLRVSEVMTKDR
                     LITAKVGIDLEESKRLLHKNRIEKLLVVDEEGRLKGLITIKDIMKIKKYPNSCKDGLG
                     RLRVGAAVGVGPDMMQRVVALTNMRVDLIVVDSAHGHSKNVIEAVKRIKSEFPDLPIA
                     AGNVATAAGAESLIRAGAEAIKVGVGPGSICTTRIVAGVGVPQISAIMECSRVAREHG
                     VPVIADGGIKFSGDVVKALAAGADVVMIGSLFAGTDESPGETILYQGRSYKAYRGMGS
                     LGAMREGSRDRYFQDEVVEATKLVPEGIEGMVPYRGPVAATVHQLLGGLRAGMGYLGC
                     RSLEDLRKKARMVRVTSAGLRESHVHDVIITKEAPNYQVDLK"
     gene            complement(23126..24157)
                     /locus_tag="PLM79_05745"
     CDS             complement(23126..24157)
                     /locus_tag="PLM79_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85844.1"
                     /translation="MDSGCNPATYASIDIGSHTIRLLVGRRLGDTLLVPLLSERRVTR
                     LAKGFQEEQTLRPSQLDLSTRVLEEYRDCMARFGVKAVACGATGVVRKSKNGRQWLRS
                     IEEVTGIQGRILSEETEAFLSAKGILGVLPAPPERVLCFDLGGSTTEFLLTDARQGHV
                     LWSSSVFIGAATITEKYLPADSASSRCLSLAGAAIREALSKKLPHPSSMGSSCPDSPE
                     GLLLVGTAGTVTTLGAMFLGMEHYEPYRVNGLELDGDWICRTLDDLAGLPVRKRKGIP
                     GLEEGREDIILGGTLIVREILRWFEKKTLTVSDAGLLEGLLFQLIEEDLGWSGGGLRT
                     PLTWGLQEG"
     gene            24265..24876
                     /locus_tag="PLM79_05750"
     CDS             24265..24876
                     /locus_tag="PLM79_05750"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOV85845.1"
                     /translation="MILEINPKNPEPRKIKKVVEVLTEGGIIAYPTDTYYGIGCDLFN
                     KGAIEKIYQLKQRSPSQPFSFICSDLKHISEYAQVTNYAYKTMKRLLPGPYTFILEGS
                     RLVPKIMLTKRQTVGIRVPDNAICLAIVHEFGHPIINTSATDPESRILQSPQELREKL
                     GHALDLIIDGGSVAGKPSSIISLIDDAPEILREGSGDIRSFQK"
     gene            complement(24880..25752)
                     /gene="sppA"
                     /locus_tag="PLM79_05755"
     CDS             complement(24880..25752)
                     /gene="sppA"
                     /locus_tag="PLM79_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOV85846.1"
                     /translation="MKRRTRWLILLLVFLVAGGILYALGSDCRIFASPNRIGVIEVRG
                     LIEDVQETVRAIGDFEKDPFIKALVVRIESPGGGIGPSQELYRALLRARDKKPVVASL
                     GSVAASGGYYIASATSRIISNPGTITGSIGVISYFPNLRELFDKIGYTTVVVKSGKFK
                     DVGYPGREMTPEEKELLQGTINEAHRQFVRDIVQGRSLPEEKVLEIADGRILMGEAAH
                     RLGLVDELGNFQDALDSAAQLGRIEGEPTLIHHKKKKRSVLDYLLGTDVGDRLASLTD
                     MTGSFLRYQMPLFP"
     gene            complement(25840..27630)
                     /locus_tag="PLM79_05760"
     CDS             complement(25840..27630)
                     /locus_tag="PLM79_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOV85847.1"
                     /translation="MVEKDETTQDTIHQAAPDEPTSPEEAGLENEMEDMDSLHDLYEQ
                     SFRNIQEGEVIRGKIVQISDDFVMVDIGYKSEGQISLHEFKDEKGIIQAAIGDEVDVL
                     LEYHDDEDGTIHLSKEKAAKIKVWDEISRIYNNDGTIEGKVVAKVKGGLAVDIGVQAF
                     LPGSQVDLRPVRNLESLIGQTLPFKVLKYNKKRRNVVLSRRALLEREREQMKSHTLAT
                     LEDGKVVQGTVKNITDYGVFVDLGGIDGLLHITDMSWGRVGHPSEMFQVGDRIKVMVL
                     SFDRDHERVSLGLKQLVADPWTQAEGKYPVGTRVEGRVVSLTDYGAFVEIEQGIEGLI
                     HVSEMSWTKKIRHPSKILSVGDVVEAVVLSINPENKRISLGMKQLEPNPWDVIAEKYP
                     VGTTIAGKIKNITDFGIFIGIDEGIDGLVHISDISWTKRVKHPSEVFKKNQEVQAIVL
                     NIDKGNERFSLGIKQLESDPWESIPERYPVGSIIVGPITNVTDFGLFVEIEEGIEGLV
                     HVSEISKEKIKSPIGQYKAGDRITAKVINISPKDRKIGLSVKKVEEQEERTNIDEYVN
                     SAKAATSNLGELLKEEMEARANSASLVDKD"
     gene            complement(27715..28407)
                     /gene="cmk"
                     /locus_tag="PLM79_05765"
     CDS             complement(27715..28407)
                     /gene="cmk"
                     /locus_tag="PLM79_05765"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOV85848.1"
                     /translation="MVIAVDGPAGAGKSTVCRLLAQRLGFAYLDTGAMYRAVAYVMTR
                     RGVGISEPSEICARLNQLPLRFALEGDQLTITFQDKRLGEELRRPEVSHLASAFSQLE
                     GVRTFLVEWQRRLGAQGNVVAEGRDTATVVFPDAAVKVFVTADLAARTRRRLAEYEAK
                     GVAVDFRTLEKEIEDRDRADQERELAPLRPAEGACILDTSHLGIEEVVSRLEEIVSRK
                     LGGDNPRCSEKK"
     gene            complement(28415..29527)
                     /gene="hisC"
                     /locus_tag="PLM79_05770"
     CDS             complement(28415..29527)
                     /gene="hisC"
                     /locus_tag="PLM79_05770"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV85849.1"
                     /translation="MKPFVPEHIAGLIPYPPGKPLEELEREYGIRDSIKLASNENPLG
                     PSPRALEAIRSTLGRLHRYPDGSGYYLRKRLSEKLDLPFEGIVLGNGSNEIIELAIRA
                     FLVPGDEVIMPAPSFLVYKLAVQTMGGKGVAVPLKDFTIDLEATLRAVTPRTKIIFIN
                     NPNNPTGTVVRKEPWDRFLRALPPEMLVVLDEAYIEFARDPDIPRGFDYIKPRGGAHV
                     LVLRTFSKAYGLAGLRIGYGVMDPGIADYLHRVRQPFNTGTLAQAAALAALDDEAFLL
                     RTRETVWDGLRYLYEEVGRLGLETLPTQANFFLIRVPVDAKRLYEALLGRGVIIRAMN
                     AYGLDRYIRVNAGIPEENRRFVEALAETLKDLQPAT"
     gene            29832..30800
                     /locus_tag="PLM79_05775"
     CDS             29832..30800
                     /locus_tag="PLM79_05775"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HOV85850.1"
                     /translation="MRPVHEPKDLDEASTRDGDTDSRAPKISVHERPSSVAFDPFRLY
                     LDEIKRYSLLSREEERELAIRYREKNDLEAAYKLITANLRLVVKIAMDFQRYWMQNLM
                     DLIQEGNVGLMQAVKKFDPYRGYKFSYYASFWIKAYIIKFIMDNWKLVKIGTTQAQRK
                     LFFNLRKEKEQLESQGIEASPKLLSHRLDVKESEIIEMDQRLNSWEISLDSPLKEDSE
                     DTHKSFLPSDDLPVDDQIADREAKAILHDKLVLFRQQLKGKEAVIFDHRLLSEEPMTL
                     QEIGDRFGISRERVRQIESRLKKKLKAYLEEEIEDLDLLQESMIEV"
     gene            complement(31066..32250)
                     /gene="nifS"
                     /locus_tag="PLM79_05780"
     CDS             complement(31066..32250)
                     /gene="nifS"
                     /locus_tag="PLM79_05780"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOV85851.1"
                     /translation="MKTLYFDNNATTRVAPEVLEAMLPYLKDFYGNPSSMHAFGGQVA
                     VRVREAREQVAALLGAQPDEIVFTSCGTESDNAAIRSALLQAPEKRHIVTSRVEHPAV
                     KALCADLALRGYRVTELPVDSEGRLDLDQYRKSLTPDTAIVSLMWANNETGVVFPVVE
                     AAQIARERGVLFHTDAVQAVGKIPIDLSKTAIDMLSLSGHKLHAPKGIGALYLRRGTK
                     FSPFLIGGHQEKGRRGGTENTPGIVALGKACELAAENLEIENTLVRRLRDRLENRILS
                     LVPNTRVNGDRFNRLPNTTNISFEFVEGEAILLMMNEYGICASSGSACTSGSLQPSHV
                     LRSMGVPYTMAHGSIRFSLSIYNTEEEVDFVLEKVPPIIETLRGLSPFWKENAKVCQK
                     TG"
     gene            complement(32247..33077)
                     /gene="nifU"
                     /locus_tag="PLM79_05785"
     CDS             complement(32247..33077)
                     /gene="nifU"
                     /locus_tag="PLM79_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HOV85852.1"
                     /translation="MWEYTEKVKDHFLHPRNVGEVEEADGVAEVGSIACGDALRFSFR
                     LDENKRIVEAKFKTFGCASAIAAASALTEMVKGMSVEEASRITNQDIADYLGGLPLEK
                     MHCSVMGREALEKAIEDYRGTAEEKPEGKVVCECFGVTDKEIERAVRENALRTVEEIT
                     HYTKAGGGCGSCHGAIEEILATLGREGIPQAPSRPGLSNIRKIRMIQETIEREIRPSL
                     KKDGGDIELVDVVGNRVLVATRGACAGCVASAQTLKHFVESKLRELVWPELIVEEVSP
                     "
     gene            33858..33992
                     /locus_tag="PLM79_05790"
     CDS             33858..33992
                     /locus_tag="PLM79_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85853.1"
                     /translation="MEWYAMQFCVTPAQDVSFFGAMQLIILAAIWVFGFRFPKPTKED
                     "
BASE COUNT         6666 a        10483 c        10079 g         6989 t
ORIGIN      
        1 gttttttgtg cactctatgc aaattttatt tgacaagatc aaacccagcc tctaggatca
       61 ctcccatgta ttttccactt ctcttctttt atgaatgcct ctcccgcggg agcggggcct
      121 gtcgggagag gcaatacttt tttcgggact tccgtctctt ccccttcgct tttgttgcag
      181 gagaaccccg ggttgaaatc ccggcggcgg gctgaagcac tcggcggcag acggtgcgcc
      241 ggggtctcgg gttcgttcca tgccggcgtc cgcacaaccc ctaaatctcc gctccccggc
      301 ccgggaaaat gcaggtcggc acatttttcc atggattcat gaaggcgagt cgggtaagtt
      361 tttgggacac gggagcctct ttgggcaaat aattcgttct caggaatagg atgggagaaa
      421 ccaatgctct tttccattca agccatcctg ggggctactt tcctttacgc cggatggctg
      481 actctggatc gccatttgac cgggggggtg gttctgatgg ccctggggct ggcctgcagc
      541 gtcgggccga tccttcaagg gctggccttt gcccggcgag ttctgcccgt ctcacccccc
      601 cgatctcccc aaaagcgaaa gtccccaaag atcatcgagc ttcagcgaca tcgttcccat
      661 ggggagcagg aaaagaacag acccacgatt cactgacccg ggcgcggaca atccccaaga
      721 aagcttcgtt cgatccgtcg gccgatcttt cagaatgcgc ttgaaggaga taccggggaa
      781 catgcttccg aagacgttga tcctgttttt ggctgtgtgg tctgcaggga cggccgtccc
      841 ctgcgggaca attgcgggga ccgaggagat atcgagagtt cagtcggccg ctgcggtttt
      901 gagtgagatc atggaaattc ccgaggattg cgttcctcct tccttgttga acaactctcg
      961 ggccgtcgcc atcattcccg gcatgctcaa ggccggtttt gtcatcgggg gacgctacgg
     1021 agagggagtc ctcatggtcc gaaattccag ggggcgatgg ggcaacccgc tcttcgtgag
     1081 gatctacgga gggagcgtcg ggtggcaggt gggagcccag gctacggacg tcatcctggt
     1141 gttcaagacc atgcgcggcg tggatgccgt gaccagcggg aaatttactc tgggcctgga
     1201 cgcctccgtg gcggcggggc cggtggggcg gcatgccgag gccggcaccg acgtccaact
     1261 gcgggcggaa atcctttcct attcgaggag ccgaggtctt tttgccggag tggccctcga
     1321 gggcgcggcc attcagatcg actacggggc caatgcagct ttttacgccc tggccggttt
     1381 gctgcccgcc cagatcatcg agagcgaaga cctcgaggca ccctccgttg cctgggattt
     1441 gagacaggtc ctgacccgct atgcggattc ctgaggatgt tgctccgtct gattgttttc
     1501 acggaatcct cgggaggatg tgtgagggac aagcctgaaa ttcccggcaa gagggattgg
     1561 ccccgggtag aacttccgga aacgcaacag tatccgtcaa tcttaatcgg cgggggagac
     1621 ggaggcgggc ggattcggat ctgcggaggg catccggatc tttctcgagg tctcccgggg
     1681 tcggggaagg ggttctgaaa gagggtccga tattctatct tggggatgcg gtaacctgga
     1741 cgggaaatcc cggagaggag ggtaagatga gggtctcatg ccggacaaag gggtttttga
     1801 ggcaatactt tttcggattc gcggcgttgg cggctgccgt gatcctttgg gtcggagtac
     1861 cccaatccta cggacagagt gcagacagtt ccaagaagca gttggagctc aattccaacc
     1921 tgttcgcctg ggccaagaag ggggacgttc aaaaggtgaa tgcctggatt tcccagggag
     1981 ccgacgtcaa cggaagggat tccgacggga tgaccccttt gatgcatgcg gcccttcgag
     2041 gacataagga agtggtgctg tcccttctcc acgggggagc cgaagtcgac ctgagcgacg
     2101 ggaacggagt caccgcactc atgcaggctt cttgggccgg aaacgtcgaa attcttcgaa
     2161 tccttcttag caacggggcg gatctgaacc tgcgcaacac cgacaaatgg cccagcttga
     2221 aaaaggcggg ggcaacgcct ttgatggcgg cggccatgaa cggaaatctg gataccgcgg
     2281 ccatcctcat cgccaacggt gcatccctgg atgaacagga cgacgacggt caaaccgctc
     2341 tcgtgatggc gacccgaggg ggccacgtac aaatggtgaa gctgttgatt cggaacaaag
     2401 ccaacacgga attgcgggac aagtacggtc gaaccgcttt gaccatcgcc gtgcagcagg
     2461 gagattcgag ggtggccatg gccctgttga aagggggcgc cgatagggag gcatccgaca
     2521 tcaaccattg gaacgcccag cagtacgcct ggtgcctggg aagagaagag atccttcggg
     2581 ccatgaatcg acaaccctaa agcccgaatt gcggcaagtc ccgccgttcc ccgggtgagc
     2641 cgggagatcc gcctgccgga tcctccccat ttgccccgag gcagactccg ggcgtcccaa
     2701 gagcgggagc tccgaggccg gatggacccg gaaaggcggg gtccctcgtt cccgtcgttg
     2761 cggcaaaggg gattgggggg gatcggccac ccccggaaaa aggtcggccc ggacaggccg
     2821 tgtccgggct tccagcagag ttatgggagt atggtgaccc cgaccctatc ggtcttgggg
     2881 tccgttgtct atcaggagtt tcctgaattt ttcatcggga aattcctgat aggacggctc
     2941 accctcgaat cgagcgtcct cgcctccccc tggaattccg accgggtttc cgaattttcg
     3001 tttgacctga gcaaagaacg tggtttacta gctccatcca accacccccc ccacaaaaaa
     3061 cataggtgaa gcccggcgaa agcggacccg acaatcgttc cgcctctttc gccacaggaa
     3121 ggatggcggt ttggccgcag gcttgagggc ctggatttat ggaaattcgc caatatgccg
     3181 aatgacggag ggatcgtgaa acgctcgagg gcttcttccc cccagcccca tgaggcaccg
     3241 gcaagcgaga tgaacgcaca cggctgttcc aagcccgacc tgcccccgac ctgccccctg
     3301 ggagttcaag actgcccggt gcggtctacc gtggaagaat tgcaggaaga atgccgcagg
     3361 ctgacggagc tctcccatac ggacccgctc acggggctct tcaaccttcg ctatctccga
     3421 gaagccatgg accaggagat ggagcgcact cgccgaaccg gcctcgccac gggcctgatc
     3481 atgatcgacc tggaccattt caagctggtg aacgatattc acggacacca gtgcggcaat
     3541 gaagccctcc ggtggacggc aaggaccctt cgccggaaca tacggcgcct ggacatcccc
     3601 tgtcggtacg gcggagagga attcgccatt gtgctgcccg caacccgcct gccccatgcc
     3661 atccgcctgg cggaaaggct gcgtcttgcc gttatggatt ctcccttgca ctacgagggg
     3721 atttgggttc ccttgcgggc cagcttcggc gtggatattt atcgcccccg ggagccgggt
     3781 tccgtgacgg tggaggattt catccgaaga accgatgaat tcctcttgca ggcaaaaacc
     3841 ctggggcgga accgggtatg ctgccggcaa ggagatggtt ccagacctgc cacggaaacc
     3901 accgctcaag agagggccgc tttgtttatc acccgatggc cgtgattccg gttcatccgg
     3961 tgaccgattc gaacacgatc ataccgcgga cagatgaggc tcgttcgcat cactcatggt
     4021 tccgggggag agaatcgtct ccggggtcgg tggacccttc cccccgctca tgaccgttag
     4081 gcggaaggca aagacatgga taggcggaat tctccggtta cgatctgtgt gagcagcgga
     4141 aagggcggcg tcggcaagac cagtttcacc gtcaacatgg ccctggcctt ggcacggaag
     4201 caacagcgag tgttggtgat cgacgccgac ctgggcctag ccaatgtgga tgtgctcttc
     4261 gggctgcatg tggagcgcac cattcgcgac ctgctggaag acggcagcga cccgatggag
     4321 tcggtgctct atctggaacc gaacctgggg gtcctgcctg ccagttccgg agtccccgag
     4381 atggtgaacc tcgatcccga cgatcaactt cggatggagg gaattcttca ggacatttca
     4441 aggcacttcg attatgtctt cctggatacg gcagcgggaa tcggaccttc ggtcctttgg
     4501 ttcaatcatt tcgccgatca caatttcatc gtgctcagcc cggaccccac ctccatcacc
     4561 gatgcctacg ccctcatcaa ggtcctgtcg cgggactatc aacgagagga tttcttcctg
     4621 attctcaacc tggtaaccaa cgaaggggag ggacgacgca cctacgacaa tgtggcaagg
     4681 gtcgtgaagc agtttttgaa gctgtccccg cagtatttcg gagcgatccc cgtggacgaa
     4741 gccgtggtga aggcaaaccg ggaacgcatt cctttcctca agctctcccc gcaatccaaa
     4801 gccgcccagg ccattgtccg gatggcggac caggtgctga aactgcagaa ggcctgcagt
     4861 cccacctagg gaattgaacc cctcggagga caagaagccc tccctcatgg ggccgtggaa
     4921 cccgcgggcc tttcatggac cccaccgttt tgtcgcctgc tccaggaaac catcgagccg
     4981 tttcccgtcc cgggcttctt ccatttcttt ccagaccgcc tcgatctcgg cgtcgatccg
     5041 ccgactgaag aaccattccc cggggatgta ggcgagcacc gcccccagga cgaagatcac
     5101 cgccatgccc tcgacggggg tcttgaacgc cttgtcgaaa ctggtcatgg aagtgaccag
     5161 ggccatgatt cccaaaagaa gggcggccgt ctcgacggct aggttgactt ccctggaaaa
     5221 gggcttccga atccagcaca agggtttcag ccaggaattc cggtcccgaa ggatggcgtt
     5281 cacaaacgct cccatagtcc cttgactcct gtggtgatcg aggtcctcga gaagcttctt
     5341 tcggaagtct ccttttcgcc ttgtggccga gaatgccagg agaactcccg caaaccctcc
     5401 caaaagggca acccagacca tttctcctct cctttctcca cccccccggt aagcgcagga
     5461 aaaatccgaa tccggagatc ggctcccggg ttcgaccacg gaccccggac catgggcaac
     5521 cctcgaccgg gtcccgccgg gagcaaaatg catccgggtc cccgagggac gtgcgatccg
     5581 gatcgcgaaa gggtgcccat ggcccctcac ccccgggggc tcctccgaac acgaaccgtt
     5641 catcctcccc tactaaacca ggtattgagc cctcgcaaga aggaagttgg ctggaaattg
     5701 ggtagcaggt ccttttgttt ttttgatatg atgccgccgt tcccgctttt cgggcgccgg
     5761 gcaatccacc ggggtcagcc gggcgagtcc atctttcgag gtcggaatgg agagcctttc
     5821 cgtcaagaat ctgtgcatct accatacctt ggatgccctc accgaaggat tgtcccactt
     5881 ttccggatcc agtcgaacgg ccctgctcta tgctgaaaaa agggaagatc ccatgcgggt
     5941 ctatgatccg caggaccttc tggccggtca cgaaccgaag ctgaaggagc tatacctgga
     6001 ttcggacctg tggcgttcca ggatcccctt cttcgaaaat aaccagctct tcggccagat
     6061 cctgccggaa agaaagctcc ccctcaccgg gttgatcacc tacggaggaa agtcccgatc
     6121 catcttctac cagatgtggt ttgcggaaca acatccggac atgtgttcca ccggtcccac
     6181 cgaacgatgg ttggaacatg ccgcttgcct cctggcccat gatttcgccc acgaggaagc
     6241 cttctacacc gggtccgccc gctacgtcct gcgggagtat tcgactcacg ccgtccggga
     6301 tttcatatgg gatgagctca accgaaggtt tggttgggac atctggatgg aggtgtatac
     6361 gatcctggat tcgatcctgg cgatttccaa gacccccgaa gagggagcct ggcctcgggg
     6421 caccctggtc tttgtggaac cctcggcttt gaagaagctg gacttcatgg tgcgctttcc
     6481 cgtcgtcgaa cgcctgggtc taagaaacta caagcacgtt cgaaagctcc tcctggcggt
     6541 ggaggactcc gaccgaaagc ttatctccga cggtcaacag gtcgtcggga tcatgaccgg
     6601 ggagcttccc atgtgcagaa tcacggcgga tttccgcggc gggcacggtt ttctgaggct
     6661 gatgggagaa cctgtttgca gcttcttcga cgggcgctac cattccacca accgcaagcc
     6721 gaagctcgtt caactggaag agttgctcct ggagtccccg gccgaccagt ccaccagcca
     6781 catcctgttc aagacggttg ccgctatcgt cgagaaggcc ggggaacgca aacacggctg
     6841 taccgtggtc atcgacttca acgatcctcc cctttccatc gcaggccaga agctggaaca
     6901 acccatcgac cttcaacaag aagcatccct ggaactggcc aagtccctgg ccaaggtgga
     6961 cggagccctg cacctgggag gggacctgcg ccttcactcc tttgcctgct tgctggacgg
     7021 acgggccgta cccggagaag accgttctcg gggtgcccgc tataactcgg ccttgaggtt
     7081 taccgcggaa agaaagaacc tgatcgtggt ggtggtttct tcggaccggc ccgtgtcggt
     7141 tattcagggc ggggtagaga tcaccgcaag cagcgaatgg gcgccctttg cccaattgat
     7201 gacccggcct cccaccctcg aggagtggct ggcccgctcc tgcnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnntcagccc ttgatcagct cggaaagaga cctcatcctc
     7381 tcttcccagt tccttgcgaa ccggggatag cttttcaggg tcctgaggga atcctgtcgg
     7441 gccttgagat aggccgccgg gtctccgccg ctggcccgaa gggccttcac agccgtttgg
     7501 ggacgatgca ccgtggtgtc gaagtgaagg atgttgatgg gatagggaag cttgtcacat
     7561 ccggaatcct cccaaatctt tcgataaatc tgccgggctc tcctttcgtc cagctgcccg
     7621 acggaacggg caatcttgtg ccggggatcc atccggcgca gggtggtttg gaggatgccg
     7681 aatcgagagg ggccttttcc gttgtcgttg gggaccacct gccttccttc ctggtcgatc
     7741 acaaaggcca ccgccgtttc gaaatccttt ttggaggcgt tcccataact tgcgatcctc
     7801 ctggcggcaa cggcattgga gacggaatcg caagcaatgc acgaaggggg aggatatgcg
     7861 gccccagtgg ggcaagcgag cttttccgag gggccttccg ccgcgggggc cccggattcc
     7921 catcccccgg cctcggccct cagactctcg taggcccgga catccccccg ggaccgggtc
     7981 aagaggtcca tggccttccg gggatcctgc agaaaggtct cgaagtgcag aacgttgagg
     8041 ggccgggaaa gcaaatcgca cccggcccgc tcccaaatct tcgtgtagat ccttgcggcc
     8101 ttttcttcgt tcaattcctt gacgcttgcg gcaacggttc cccggggatc gtattcccga
     8161 agagtggatt gaagaatgcc gaatttcatg ggcggcttgg acgattgccc cgccgggtcc
     8221 ttgaggatcc ggtccaatac cccggccagg tccgtccccc ccggaacttc cgaagaaagg
     8281 gtccgtgaga aggagtcctc cggcttctta tccgatggag cggcctccca cccgggaggt
     8341 tcctgaattc ttcccaaact cgatacatga agcatggaag cgatcccttc tttggtgaaa
     8401 ggttagcccg gcacaatccc ccgcaggaga tcgtagtcca aatcttgtgc caaccgtcct
     8461 cgaacgggag gggctttcga tgcgaccttc gggacatccg gcccccggcc gcggatcccg
     8521 caccctcgaa gactctcccc ggcctgccgc catcgagaag ctttcccgga tcccgggtcc
     8581 tccccacaca gtcgaattcg tggttttccg tcggccccgg agggcaacgg gaaaatctgc
     8641 gacaagtttt tgacggcacc gccccccgtt ccccggaatc ccggtttttc ccgcaacccc
     8701 gcgggggggc ggctcggccc gtcatcggaa tcgcttttcc cgctcccgaa cggccgggac
     8761 cccgatgaac tcgaagtact cgtcaaagct caacatctgg tcccggtaac cgcgggtggt
     8821 cccgtgctcc ttgaggtagc ccgcccacct ctgcagggcc ttcacggcgg caaagacgga
     8881 gccgcaggga taggcgacca cgctgtagcc caactcctgc agctccccgg cactcagaaa
     8941 gggggtccgt cctccctcga tcatgtttgc catgctcggg gtattggatt ccgccagggc
     9001 cgagttcacc tttcgcatgt cttcagtggt gtgtaccgct tctacaaaga tcatgtcggc
     9061 cccggcttcc gcataaatcc gggctcgccg cacggcctcc tcgatgccgt agacagcaca
     9121 ggaatccgtc cgggccatga tcaccaggtc cggatcttcc cgggcccaaa gggcggcttt
     9181 caacttcggc agatattcct ccaccgacac gacgctcttt cccgccatgt gaccgcaccg
     9241 tttgggatac gtctggtctt caaggaaaag ccccgccacc ccggccttct cacaatccct
     9301 cacggctcga atgacgttgt tcacgtctcc aaaaccggta tccaggtcca ccagaacggg
     9361 gacgttcacc gcactgcaga tgtgcccgta ttgggtgatc atctccgagg cggtcaacac
     9421 cccgatgtcg ggcatgccga gaaggctgcc ggaaagaccg taaccggccg ccgcaatggc
     9481 cttgaagccg gactgttcga gcacccgggc cgaaagaccg tcgtggcagc agggcaagag
     9541 aaggatctcg ggatcctgaa tgagcttcct gaagagggtc gtctttttca tcgcatctcc
     9601 cattttgtga ttcgaaccag aacggtcccg ccgggcatga cccggtcgag aagaaagacg
     9661 caggtcgttc caactcccgc gcgactccgg gttcacccgc ccccgtagac ttcgtcggcc
     9721 tctctactct actcctcttc gcccttccct gaaacggttt tgggagctgg acgacgaggc
     9781 cgcttttact gccgggggag ggcacgaaga cgattttgcg gggggagaaa gcccgtcaaa
     9841 atgcccagag agattgaaat gccgactccg caaggctaga atgatcgcgc gaaatcttga
     9901 ttcacggccg ggagatgagc cacccatgcg atccggaacc ctcatgaaaa tgcttttggg
     9961 aatcctgttt ctttgcccgg gtttcctcct tttttcatat gcctccgttc tccgcgtcca
    10021 cggcgccgag gtcctccggg tcacggacga ttcggggcaa accgtcgagc ttccgcaacc
    10081 cgctcgacgg gtcattcccc tttacggggc cttcgcggaa atgctatatg ccatcggtgc
    10141 cggagactcg gtggcggccc gcacccaggc ggatcgtttt cccccggacc ttctccggct
    10201 cccgtcggtg ggaactcaca tgaagcccaa cgtcgagatg atcctggggc tgaaacccga
    10261 cctggtgatc cagagctcca gccggaggga agcgatcccg gaaatccgga ggatccaggc
    10321 ggcgggaatc cccctggtcg tgttcgatcc caagagtttc ccgcagatct tttccaccat
    10381 gatcaagatg ggagtcctca cggggaagga aggctcggcc cgggaggcgg tcgagcagct
    10441 cgaaggccgc ttgaaaaagg cgggaatccg gcttgaaggg agcacaagag agctccgagt
    10501 gttctacgag gtccgtgggg aacccctcac ggcggcggga cagggatcca tcatccagga
    10561 gatcctcgtc gctgcggggg cgaaaaatgt cgtctcctcc cccaaggcct tggtgcagta
    10621 cagcgtggaa gccctgctgg tggacgaccc cgacgtgtac attgtccagc gaggccccat
    10681 gaaccggaac cctgctcccc ccgaccggcg ccagcatttc tcccgtctgc gctgcgtgcg
    10741 cgaaggacgg gtcctctttg tggacgaatt catcttttcc cgcccgggtc cccgttgcgt
    10801 ggatgccgtc gagcagttgg cagagaagct gcgaccctat ggaaagtcat cctgagaacg
    10861 acatgaaatc caccgaccga cccgtttcca aacgccggaa gcccaagaag catcggctgc
    10921 gcatgggctt cagcaccggc accgccgtga cggccgcagc ccaggcggca atgaggcacc
    10981 ttctgaccgg cgaatccccc agagcgattt ccgtgaggct ccccgcgggc tactacctcc
    11041 ccatccccgt tgaggaaacc caaagaaacg ggacctcagc cggggcccgg gtcatcaagg
    11101 acggggggga cgaccccgac gtcacccaca aggcggaagt gagggcccgg gttacctgcc
    11161 ttcgatccac gggaaccgac ccaacggacg cggccgtggt ttccgagcaa tcttccccgg
    11221 gggtgtggct gctgggagga gagggggttg ggctcgtcac caaagcgggc ctgcccgttc
    11281 ccatcgggga gcctgccgtg aatccaggcc cccggcaaat gctcctggca ggactgaccc
    11341 gagagctttt gcgggtggat gccgacctca tcacccgggt cgtttccgac gaaagaatcc
    11401 ccaactcttc ccatctgtcc gacggccggg tgtttttgcc tttcttggag gaggattctc
    11461 gattggccgg gctgtccgtt tgtctcgaga tttccgtccc ccggggaaaa gagctggccc
    11521 gccacacttt gaatccccga ctggggatcg tcggaggcat ttccatcctg ggaaccacgg
    11581 gtttggtgaa gcccttttcg caccaggcct acgaagaaac catccgggtg agcctcgcgg
    11641 tggcgcattc cagccgttgc cgaacggtgg tgttgagtac cgggggaaag agcgagcgtt
    11701 acgcccagga tgccttgccg tttttgcccc ccgaggcttt tgtccagatc gcggattttt
    11761 ttgcctttgc cgttaaggaa gccgcagccg cgggtttcga ggaaatcgtt cacagcgtct
    11821 ttttcggcaa ggcggtaaag atggcccagg gacacgctta cacccacgcc cacaaggttt
    11881 ccatggacct gctgcccctg gctcgcctgg ccctggaccg gggctatggt gccggttttt
    11941 ccaggcaaat cgaggaagcc aatacggccc gccacgccct cgagatcctg cggcaggaac
    12001 aggcgtggga cctggttcaa atggtggccg tccaggcccg ggaccggtcc cgcaaggcat
    12061 gtcgtcccgg cacccgggtg aggcttctgc tcttcgacta tgacggttcc ttattggcgg
    12121 acatccggga tgagggagac aagggatggg cataaccccc gagggttggg agccgccgct
    12181 ggtcgtactc atcgggatgg gaacggatcg ggaggatctc agcctgcgtg ccctgcggtg
    12241 gattgagcag gcggaggtgc tggccggggg aaggcggcac ctggcgggat ttccgaaaca
    12301 ccccggcgaa aaaatccccc tcgagtcccc cctgggaggg gcactcgaga agatccaacg
    12361 ggtttcccgg gaacgaaaga ccgcggtcct ggcctcggga gatcctttct tcttcggcat
    12421 cggtcccctc ctcgcccgac aggtggggga agaaaggctc ctgaccctgc ccaacgtaac
    12481 gagtccccag gtgctcttct cccgcgcggc ccggccttgg gaccgcctgc agaccgtgag
    12541 tttccacggg agggggtccg atcctctcga ccgggattgg cttcgcctgg ttggaagtcg
    12601 gatgcccgtg gcctgtttta ccgacgaaga ccattctccc gcgtggatcg cccggcaact
    12661 cgtcgaggcc gggcaagggc attgcaccct cctggtgggg gaggacctgg gctcgatccg
    12721 ggaaagaatt cttcgcctgt ctccggagga agccctgggc cgggaatact cccctttgaa
    12781 cctggtggcc gttctccccg gggaggaagg ggggccggcg gacccgcacg gggtggaaaa
    12841 ggaagaaggc gaggcagccg tattcggact cgccgaagaa gcctttcgac accgggcggg
    12901 gatgatcacc aagagcgaaa tccgggccgt agtgctggcc caactgcggc tcgagccggg
    12961 attgaccttg tgggacctgg gggcgggcag cggttccgtc gccatcgagg catcgagggc
    13021 cgtcgccctg ggggaggtcg ttgccgttga aaaggatgcc gcgaggttcg cggacttgct
    13081 gcacaatgtc cggcggctgg gcagaggaaa catccgtgcc gttcacggag atgtaggggc
    13141 cttggtggat ggactgccgg accccgaccg ggtgttcatt gggggaggcg ggaagtccat
    13201 ggaatcgatc ctctcgaaga tcctgggaaa acttcggccc cggggacgag tggtgcaaaa
    13261 cgtggtgacc ctcgagagcc tctcccgggc aagttccttc tggcagagtg cgtcccggga
    13321 attttccgtc acccaggtgc aggtcaaccg ttccgttccc ctgggaaata cttttcggtt
    13381 cgaaccgctc aacccggtct ttgtgatcac ggcttgggcc aagtgaggat gggatgaagc
    13441 atcctgtggc atttgtggga gcgggccccg gggacccgga gctcataacg gtcaaggggc
    13501 ggcggctcct gagggaggcc gattgcatcg tgtacgcggg atctctcgtc cccgaatccc
    13561 tgctgggctg ggccagaccg gaggcccgca ggcacaacag tgcgtccttg accctcgagg
    13621 aaacccatgc cctgctcaag gaagcttgcc tggcgggcga ccgcgtggtc cgactccaca
    13681 cgggagaccc cagcctctac ggggccatcc gggaacagat ggcgctcctc gagaaggacg
    13741 ggattcccta tgaagtggtt cccggcgttt ccgccgtttt cgccgcggcg gcgtccctcc
    13801 gaaaggagct cacccttccc gggatatccc aaaccgtgat tcttacccgc ctgagcggca
    13861 ggacccccgt tcccgagccc gaggcacttc ggtccctggc gacccaccgg gccaccatgg
    13921 ccgtttacct gagcgtgcag gagatggaga cggtggtttc ggagctggcg ttttcctatc
    13981 ctccggagac tcccgtggtg gtggcatacc gggtgggctg gcccgatgag ctcctccttc
    14041 gagggaccct cgaggacatc gcttcccggg tgaaggcttc gggaatccgc cgccaggctt
    14101 tgatcctcat cggaggggtc ctcgatcctc ccgacgatcc cgaggcccct cttcggtcca
    14161 gactctacga cgaaaccttt tcccatggat atcgagaagc caaggaccgg gtctgagtcg
    14221 ccgacggcca tcgtcgcact cacccgccac gccgttgaac tggctctgcg gctgcagccc
    14281 gagcttccct cgagtgtgtg ttacgttccc gtgcgccatc gctttgccct ggccatgggg
    14341 gcccgggggt tccaacgatt gggcggcctt cttggggaga tctggccccg gtattcggcc
    14401 tttgtcttca tcatggccgc cgggatcgcc gtgcgccgga tcgcgccgct catcaagagc
    14461 aaattgtcgg atcccgccgt ggtggtcctg gacgaacggg gccaattcgt cgtcagcctt
    14521 ctttcgggtc acctgggggg agccaaccag ctggctttga aagtggcccg gctcctcggg
    14581 gctcagccgg tcatcacgac ggcctcggac gtttggaaga agcccgccat ggacctggtg
    14641 gcccaggagg cgggcctcga ggtggaaaac cccgaaatgc tggcccggtt gacccgggca
    14701 ttcctcgagg acgaagaggt atgggttttc gaccccgagg ggaggatcga ggattctttg
    14761 ggcgactatc cccatgtgcg tcgtctgagc ccgggagaac ccgaaaccgt gcgggagcgt
    14821 ccgggagacc cgggtgcccc gccgccgggc ctctgggtcc acgaaaccgt tccttccccg
    14881 gaaggcatcc ggtttctgct tctccgacct caaaacctgg tcgtcggcat cgggtgcaac
    14941 cggggaaccc ccatggagga gatgcgggac tttctcgagt ccgtcttccg ggagcaccgg
    15001 ctttccctga ggtccgttcg gaacctggcc agcgtggact tgaagtcccg ggaagaggga
    15061 atgatcaagc tggcggaaac ccttcggcgg cctatccgtt tttactcccg ggaggagttg
    15121 agaaacgtct ccgtccccaa tccgtccctt cgggtggcaa atcatatagg agttcacagc
    15181 gtatgcgaag cagcggccat tctcagtgct caaagcccca cccttctcgt gcccaagcaa
    15241 aagaccccca acgtgaccct ggccgtggcc cgggcagact gtccgtcgtg agcctgggac
    15301 cgggagaccg gtctctgctg gtggccgaag ccctggaagc cctccgcgag gccgaggtca
    15361 tcgtgggcta caccacctat ctcgagctca tcccggacct cacccccggg aagaaagtcc
    15421 tgggaagcgg catgcgaaag gagattcccc ggtgccgggc cgccatcgaa cacgcccgaa
    15481 acgggcatcg tgtggccctg gtgtccagcg gggatgcggg aatctacggc atggccggcc
    15541 tggtgctgga cctgtgccgg gactcgggcc tccgggtttg ctccgtccgt gaaaaaccgc
    15601 cggaaacctg tgatctgcag ttcgaggtga ttcccggggt cgccgctttc aacgccgccg
    15661 cctccctggt gggggccccc ctcatgcacg attttgccgc cgtgagcctg agcgatcacc
    15721 tgactccctg ggaactcatc gaaaaaaggc tgcaggccgt ggccgcggcg gatttcgtca
    15781 cggccgtcta caaccctcga agcaaggccc gccccgacct tctcgagaag gcctgcgaga
    15841 tcctcctcga gcaccggtct cccgaaaccc ccgtggccat tgtgcgtcga gccaaacggc
    15901 agggagaatg ggttcacctc acaaccctcg accggattcc cttcgacgag gtggacatgc
    15961 agtccattct tctcattgga aacagccgaa cctacctgtg ggaccggtgg atggtcaccc
    16021 cccggggcta cctggacaaa tacggagaag gggcggaagg cgaagggcgg gaagccggga
    16081 gtgaaaggct gagggcgcgg agactcgacc attgcggccc cgatgcggaa tccggctgat
    16141 accgggttgc gcccagggtc gccctggggt gctcagtgaa aatttcgagc aaagggctga
    16201 gtcctgcgga tgggcagttt cggcttgagt gcaacttcgt aggagaaccc cggatgtggg
    16261 tgcctgccta cgaccgaatc ccgggttcgg atgggtgggg acgggaaagt actccgtgag
    16321 attccggcca tccgccgccg gaaaagagga tcggcctccg tcgccctcga tttggcgggg
    16381 ggaagccggt tgtcatcatg accgggaggg tcaggaaaaa aacgtgtcct gctgggaggc
    16441 catcacctgg gagacttcct ccagcagctt ctccacagcc tcaggggtta gtttcagtgc
    16501 ccgggcgcta accaccgtct tcatgtccag gtccgggttc cgggtgggtc caatttccag
    16561 cttgtggcac aaggcatttg ccaggtgaac gatgcagcag accaccggcg ccaccttggc
    16621 ccgttgggga tggtggtgat ttccgatggt ttcctcgatc tctttggcga agttccattt
    16681 ccgggcgatc tgttcgccga tctgggcatg atcaaacccc agggtcctca attccaccgc
    16741 cctgaagccg gtttttccgg ggctgttgta aacctcctgg acaatctcca gcatttggtc
    16801 cggcagcttg agattcagca ccacttttcc gatatcgtgg agcaatcccg ccaggaaggc
    16861 ctcctccgtt ttgggatagc ggaggagggc cgccacccgt ttggaggcgg cggcgcaacc
    16921 gatggagtgc tcccacaaga gtttttccgc cagcccgaaa ttcttgaaaa aatcctgtaa
    16981 taccgaggtc atcaccaggg atcggatgct gttgaatccc atgaggacaa tggcgtccgt
    17041 caatcgggtg atgttcttgg cacaaccgta gaacggggaa ttggcgatcc tcaacaccct
    17101 ggcggccaag gcctggtcct gggagatgag ctggtggagc tgcttggccg tggcgttggg
    17161 atccgcaagc ttgtccagca ccatgttggc cacggcgggc atggtgggca gatcgcccac
    17221 cgttcgaacg attcggtcca gtagagtgga ttcagcaggg gtcttttccg gttccatagg
    17281 ctccttcata cccacggttc agcttttgag gtgaggcgag ggtcccgatt cctgccggga
    17341 aacgccgctc tcctgacgtc agagaggttt ttgaaccccg aatttgcggc aacggactcc
    17401 cttggcccat catggcaggg gacacgcgtg cggatgggat ggatgacgtt ggctcgcggg
    17461 agaaagcgcg ccacacacgg ggaaggaaca gcggctcgcc cggcccggca gcgttccagc
    17521 ccctttcggc ttcttcgcgg caatggcggg agactcctcc aaggacccag gatcaaaacc
    17581 ggtctcgcag caaattcccg gtgccctatt gggcacgatc ggcctcggaa ccggcttcga
    17641 cctccttggt cttctttccc tcgactgcaa gacgccttgc acggaaatac ggcttgagca
    17701 tcagcccgaa tcccacgatg aagcccagca ccaaggaaga aaacagaagg gtacaaaggg
    17761 aaagcgtcca gttcaacggt tgtcggagat acaggttcag ttgaaacggc acgggagtct
    17821 gaaaagcaga aacattttgc cagaagaaca gcgcaacaat cgccaggagc agaccagtcg
    17881 tgaacagctt aaagagcttc atcagcactc tccttcttga atttgggcaa tagcttggca
    17941 taggtctgtt tcaagtgttc gggaatactc ctcagttccg caaagaccgt taagaagttg
    18001 gtgtctccct gccatcgagg caccaggtga aaatgcaggt ggtcctcgac cccggctccc
    18061 gccgccgaac caaggttcaa tcccacgttg aagccgttgg ggcgcatgac cgatttcagg
    18121 atgaaaatgg acttctgcaa ccatttcatg atggaaaggg tctcttcctc ggtaatttcg
    18181 tccagggcgg ccacatgacg ccagggggca atgagcaaat gtccgttgtt gtagggatac
    18241 ttgttcatca tgaccatgca gagtcttccg cggtgcagaa tgagccgttc ttcgtcgctg
    18301 accccgtgcc cttcgggaca aaaaatgcag tagggctcac gtttgcccaa aatatattcg
    18361 attcgccacg gcgcccacag gttttccaaa aaaatcactc ctgcagagcg atgatctcgc
    18421 ccgtcagttt ctcgttcacg tgaaggatcc ccaggctgtg ggagagtgcc cccctgccca
    18481 aggaaacgga tcccggatta aaccagaaaa tgccgtcctc ttcaaggagc atgggctgat
    18541 gggtatgtcc gaagaggatc gcgtccactg ttgggaaatg atgtgccaat cttgcccgga
    18601 tggcatggtg agatccccag ccgtgggcga ggccgactcg aaacttcccc agtcgcagga
    18661 ccttggtcgg gggaagtcgc tcctggattg ctgcggggtc catgttcccg gcaacggctt
    18721 ccaggggata ttgctcgagg tagtccaaga tcccggtgct tacgaggtcc cccaggtgga
    18781 tcaccaggtc cgcatccttg ccgtagcaat cacacagggc acggaagtgg tccgtcggcc
    18841 gggaaaggtg ggtgtcggac atgaccacga tcttcatggc agctcgagct ctcatggatt
    18901 ttgcgcgaac tcggcacggg cgggcgatgt tgcttgcgga cgccctgcct gcgggaattc
    18961 gggttttcgt ccccgggatc aagccggtat gcccgctcca gccggcggaa aatcttcccc
    19021 tccgggcccc ggtatcgaaa tccgaatcct cccgaacact tactatagca agcaccggcg
    19081 caaataggaa ctaatttcta cgaagtcccg ggaggggccg aagccttccc caggccgggg
    19141 aggacggagg aaatccgggt ctggataaag gcttgaggcg ggatcgaggg agactcacag
    19201 gggaaggagc tccaggtctc cccacacggc catccggtcc ttgatgagaa tcagcacccc
    19261 ctcgatcgag gcaatctcgg ccgcttcctc gagggtcttc tcgaggtcgg ccgaggtctt
    19321 cacccggttc cccagggccg tggcggcaca atcggcgagg accacgtccc gggagaccac
    19381 ggtgacggca tcggcccttc ccaggctgag agaatgcccc accgtggccg aagaggtaca
    19441 gatccccaga ggaaagcgat ccgctgtgaa ccgcaatccc aggcggccgg tgaagggaga
    19501 atggggaccg gcaaagatcc cgacggtcgt ctcttccgcc aaatccatgt agcagtcccc
    19561 tccgttctcc accacgatgc cgggactcag gggcttcaaa gaaagagccg tcgcctcggc
    19621 gacggccccc gccaccccgg ccatgggtcc gacccccgcc gcccgggcgg cctcgatcat
    19681 ccggcgcgcc gggggcggcg ccgtaaggtc atcggggata gggaccaggg atttcaggaa
    19741 ttccgggcgt gccgcgatat attcgtccaa ctggtggcgg cattggagaa cacaggtgag
    19801 ggcttcttgc ttcaaggaac gctgggctcg aatccagagg tcggtctcct tgtggcgaac
    19861 ctggaaggtg acccatcctt gcctcttcga gtgctgaacc cggtaaaagc ggcgggggtt
    19921 tgtatagggc ttcttcaagg attccctcac cctgtttggc ccgcggggtc cggggacccg
    19981 ggtcttgccc tattcccatt cgatggtgct ggggggtttg gaagaaatgt cgtacaccac
    20041 gcggttgatg cccttgactt cgttgatgat ccggttggat atcctctgga gcaggtcgta
    20101 cgggagcttg gcccagtcgg ccgtcatggc atccacgctc tccaccgccc gcaacaccag
    20161 ggcctgctcg taggtcctct cgtctcccat gacccccacg gaacgcacgg gaaggagaac
    20221 tccaaaggcc tgccaaaccc ggtcattcca ttgggcaagt tccatttcct cgagaatgat
    20281 gtggtctgct tccctgagga tttccagttt ttccgaagtc acctccccca ggatgcgaat
    20341 ggcaagcccg gggccgggaa aaggatggcg caggatgatt cgttccggca gccccagctc
    20401 cctgcccacc aggcgcacct cgtccttgaa cagctccctc aggggttcga tgagctcgag
    20461 gtccatccgg tcgggcaatc cccccacgtt gtggtgagtc ttgatggttg cggacggccc
    20521 cttgaaggag acactctcga tgacgtccgg gtagagggtc ccctgggcga ggtacttggc
    20581 cgggggaagt ctccgggctt ccttttcgaa cagctcgatg aaaaggttgc cgatgatctt
    20641 tcgcttggct tccgggtccg taacccctcg gagtcgatcg agaaagagat gcccggcatc
    20701 caggtaggtc aggttcatct cgaaatggtc ccggaacacc cggcgcacgc tctccgcctc
    20761 ccccttccgg agaactccgt tgtcgacgaa cacgcaacgc aactgggaac cgatggcgtt
    20821 gtggaggagg accgccacca ccgaagaatc gacccctccg ctcagggcgc aaatcacgtg
    20881 atccgaaccg atcttctcca ccaggctgcg ggtgaccgac tccacaaaag acttcatggt
    20941 ccaggtggga cggcagcggc atatgccgta gagaaaattc tccaggaggg tcttgccgca
    21001 gggcgtatgg accacttccg ggtgaaactg gacgccgtaa aagcggctct cggggtcggc
    21061 catggcggca atgggggaat tggaactgct tgccaggacc cggaaccccg gaggaagctc
    21121 gaggattcgg tccccgtggc tcatccagac ccgcacgcct tcgggctcga tatcccgaaa
    21181 gagcttctcg gggtcttgga cctcgaccag ggcatgaccg tattcccgcc ggtcggccct
    21241 ttccacggcc cctcccaact gccgggccat gagctgcatt ccgtagcaga tacccaggac
    21301 ggggatgccc aggccaaaga ggccgggatc ggaaaacggg gcgttcagat cgtagacccc
    21361 ggcgggtcct cccgaaagga gaatcccctt gggcccaaaa gcgcggatct tttccagaga
    21421 caggttgaag ggatgaatct cgcaatagac gtggctctcc cgcacccgac gggcaatgag
    21481 ctgggtgtac tgggaaccga agtcgaggat gaggacacgg tcctcatgaa gagggtcgat
    21541 cgtcatgaca ttgttccttg gcaaggcacc ccgcccaagt gggaatgtta tttcagatcc
    21601 acttgatagt tgggcgcctc tttggtgatg atgacgtcgt gaacgtggct ttcacggagt
    21661 ccggcgctgg tgactcgaac catgcgcgcc ttctttcgca ggtcctcgag actgcggcaa
    21721 cccaggtagc ccatcccggc ccgcagaccg cccaggagct gatgaaccgt ggccgcaacg
    21781 ggtccccggt agggaaccat cccctcgatt ccttcgggaa ccagcttggt agcctccacc
    21841 acctcgtcct ggaaataacg gtcccggctt ccttctctca tggctcccag ggagcccatt
    21901 ccccggtagg ccttgtagct gcggccctgg tagaggatcg tttcccccgg actctcgtcc
    21961 gtccccgcaa aaagactgcc gatcatgacc acatcggccc cggccgcaag ggccttgacc
    22021 acgtccccgc taaacttgat gccgccgtcg gcaataacgg gaactccatg ctcccgggcc
    22081 acccgggagc actccatgat ggcggaaatc tgggggaccc cgacccccgc cacgatccgc
    22141 gtggtgcaga tggagccggg tcccactccc accttgatgg cttcggcccc ggcccggatg
    22201 aggctctcgg cccccgccgc cgtggccacg ttgcctgcgg cgatcggtag gtcgggaaac
    22261 tcgctcttga tgcgcttgac ggcttcgata acgtttttcg aatgtccgtg agcgctgtcc
    22321 accacgatga ggtccacccg catgttggtg agggccacca cacgctgcat catgtccgga
    22381 ccgactccca ccgcggctcc cactcgaagc cgtcccagcc cgtccttgca ggagttggga
    22441 tacttcttga tcttcatgat gtctttgatg gtgatgagac ccttgagcct tccttcctcg
    22501 tccaccacca ggagtttttc gatgcgattc ttgtggagca gccgcttgga ttcttccaaa
    22561 tcgatgccca ctttcgccgt gatgagtcga tccttggtca tgacttcgga caccctgaga
    22621 tccaggttgg tctcgaagcg caggtcgcgg ttggtaatga ttcccaccag ctggcccgct
    22681 ttcaccaccg ggaccccgga gatgtgataa cgggacatga gatcgagaac atcggaaatc
    22741 ctctggtcgg gctccaccgt caccgggtcc accaccatcc cgctctcgga ttttttcacc
    22801 ttgctgactt cctgggcctg gcgctcgatg ggcatgtttc gatgaacgat tccgatgcct
    22861 ccctcccggg cgatgctgat ggccaggtcg gcctcggtga ccgtgtccat ggcggcgctc
    22921 gcaatgggga tccgcatggg gatactccgg gtgattcgcg tttccacgct ggtgtcgttg
    22981 ggcaggactt ccgagtattg gggaagcagg gaaatatcat cgaaggtgag tgcctcggga
    23041 acatggtcgg catacttttc gttcattgaa cctctccatc gttgtcttcg gttgttggcc
    23101 cacgtcggcg tctcgtgaac ggcctttacc cctcttggag accccatgtc aagggggtcc
    23161 tcaacccgcc gcccgaccac cccagatcct cctcgatgag ctggaagaga agcccttcga
    23221 gaagccccgc atcgctgacg gtcaacgttt tcttctcgaa ccagcggagg atttccctga
    23281 cgatcaaggt ccctcccagg atgatgtctt cccggccttc ttcgagtccg gggattcctt
    23341 tgcgttttcg gacgggaagg ccggcaaggt cgtcgagggt tcggcagatc cagtccccgt
    23401 ccagctccag cccgtttacc cggtagggct cgtagtgttc catccccaga aacatggccc
    23461 ccagagtggt gacggtcccg gcggtaccca ccaggagcag tccctcgggg gaatccgggc
    23521 aggaggaccc catggaggac gggtgcggca gtttcttgga cagggcttcc ctgatggcgg
    23581 cccctgccag ggagaggcag cgggaggagg ccgagtcggc cggaaggtat ttttccgtga
    23641 tggtggcggc gccgatgaaa acactggagc tccaaaggac atgcccctgc cttgcgtccg
    23701 tcaggaggaa ttccgtggtg cttcctccca agtcaaagca gagcactctt tccgggggag
    23761 cagggagcac cccgaggatc cccttggccg aaagaaaggc ttctgtttcc tccgaaagaa
    23821 tccgaccctg gatccccgtg acctcctcta tcgaccggag ccactggcgg ccgtttttcg
    23881 atttccggac cacgcccgtg gcgccgcacg ccacggcctt caccccaaag cgggccatgc
    23941 agtcccggta ttcctcgagg acccgggtac tgagatccag ctgggacggc cggagggttt
    24001 gctcctcctg gaatcccttg gccaggcggg ttaccctccg ttccgaaagg aggggaacca
    24061 ggagggtgtc ccccagacgc cggcccacga gcaaacggat ggtgtgggac ccgatgtcaa
    24121 tggaggcgta ggtggcggga ttgcagcctg aatccaagct cctgttcctc ttcttggctt
    24181 gactttgctt tgacgcaacg gttattcata aatcggatcg tcccaccgat ttcctatgag
    24241 ggaacccacg gaggaggctt gcctatgatc ctggaaatca atccgaagaa tcccgagccc
    24301 agaaaaatca aaaaggtggt agaggtcctg accgaggggg gaatcatcgc ctaccccacg
    24361 gatacctact acggaattgg ctgcgatctg ttcaacaaag gagccatcga aaaaatctac
    24421 caactcaagc agcgttctcc ttcccaacct ttcagcttca tctgcagcga cctgaagcac
    24481 atcagcgagt atgcccaggt aaccaactat gcttacaaaa ccatgaaacg tcttcttccg
    24541 ggcccctaca cattcatcct cgaagggtcc cgcctggtcc ccaagatcat gctgaccaag
    24601 cggcagaccg tcgggatccg ggtgccggac aacgccatct gccttgccat tgtccacgaa
    24661 ttcggacacc ccatcatcaa taccagcgcc acggacccgg agtccaggat cctgcagagt
    24721 ccccaggaac ttagagaaaa actgggccat gcgttggact tgatcatcga cggaggaagc
    24781 gtagcaggaa agccctcgag catcatctcc ctcatcgatg atgctcccga gattttgcgc
    24841 gaaggaagcg gagacatccg ttcgttccaa aagtagaggc taggggaaca ggggcatttg
    24901 ataccggaga aagcttccgg tcatgtccgt cagggaggcc agccgatctc ccacgtcggt
    24961 ccccaacagg tagtccaaca ccgagcgctt tttcttcttg tgatgaatga gggtgggttc
    25021 cccctcgatt cggcccaact gagcggcgga atccagggca tcctggaagt tccccagttc
    25081 atccaccagc cccagacgat gggcggcttc ccccatgaga atccgcccat cggcaatttc
    25141 caagaccttt tcttcgggca ggctccttcc ttggacgatg tcccgaacga attgtcgatg
    25201 ggcttcgttg atggtcccct ggaggagctc tttttcttcg ggggtcattt cccggccggg
    25261 atatcccacg tccttgaatt tcccgctctt gaccaccacg gtggtatatc ctatcttatc
    25321 gaacaattcc cgaaggttgg ggaaatagct gatcaccccg atgctccccg tgatggtccc
    25381 gggattggaa atgatccggc tggtcgcgga tgcgatgtag tacccccccg aggccgccac
    25441 ggatcccagg gacgcaacca cgggtttctt gtcccgggcc cgcaggagtg cccggtagag
    25501 ttcctgggaa gggccgattc cgccgccggg cgattcgatc cttacaacca gtgctttgat
    25561 gaagggatct ttttcaaaat cgccgatggc cctgacggtt tcctgcacgt cctcgatgag
    25621 gccccgcacc tcgatcaccc cgattcggtt gggcgatgca aagattctgc aatcggaacc
    25681 caaggcatag agaatgcccc cggcaaccag gaacacgagg agtagaatca accagcgggt
    25741 tcgtcgcttc acgatgttcc ctccaaaaac aaacacccgg tttcaggcct gaggcatgaa
    25801 ccgggtgtgc ggtttgatca gtcgagaagc agcttcagac tagtccttgt ccaccaagga
    25861 tgcactgttt gcccgggctt ccatttcttc cttgaggagt tctcccaggt tggacgtcgc
    25921 agccttggca ctgttgacat attcgtcgat attggtgcgt tcttcctgtt cttccacctt
    25981 cttgacggac aggccgatct tccggtcttt gggagagatg ttgatcacct tggcggtaat
    26041 ccggtcccca gccttgtact gcccgatggg cgacttgatc ttttccttac tgatctcgga
    26101 gacatggacc agcccctcga tgccttcctc gatctccacg aaaagcccga agtccgtgac
    26161 attggtgatg gggccgacga taatgcttcc cacggggtag cgctcgggaa tgctttccca
    26221 gggatcggac tcgagctgct tgatccccag ggagaaccgc tcgttgccct tgtcgatgtt
    26281 caggacgatg gcctggactt cctggttctt tttgaagacc tccgaggggt gtttcactct
    26341 cttggtccag gagatgtccg aaatgtgaac cagcccgtcg atgccctcgt cgatcccgat
    26401 aaagatccca aaatccgtaa tgtttttgat cttcccggca atggtggttc ccaccgggta
    26461 tttttcggca atgacatccc agggattggg ctcgagctgc ttcattccca gggagatccg
    26521 cttgttctcg ggattgatgc tcaacaccac cgcttccacc acgtcgccta cgctcaggat
    26581 cttggacgga tggcggatct tcttggtcca cgacatttcg gacacgtgga tgaggccctc
    26641 gattccctgt tcgatttcca cgaaggcgcc gtagtcggtc aggctcacca cccggccctc
    26701 cacgcgggtc ccgacggggt attttccttc ggcctgggtc cacggatcgg ccaccagttg
    26761 tttgagcccc aaggacactc gttcatgatc ccggtcgaag ctgaggacca tcaccttgat
    26821 gcggtctccc acctggaaca tctccgaagg atggcccacc cgaccccagc tcatgtccgt
    26881 aatgtggagg agcccgtcga tgcctcccag atccacgaag acgccgtaat ccgtaatatt
    26941 cttgacggtg ccctgcacca cttttccgtc ttcgagggtg gccagggtgt gggacttcat
    27001 ctgctccctc tcccgttcca gtaaggctct ccgggacagg acgacgttac ggcgtttctt
    27061 gttgtacttg aggaccttga acggaagggt ctggccgatc aaagattcca ggttgcgaac
    27121 cggccgcagg tccacctggg atcccggcag gaaggcctgt acgccgatat ccacggccag
    27181 acctcccttg accttggcca ccaccttgcc ttcgatggtg ccgtcgttgt tgtagatgcg
    27241 gctgatttcg tcccatacct tgatcttggc ggccttctcc ttggaaagat ggatcgtgcc
    27301 gtcttcgtcg tcatggtact ccaggagcac atcgacctca tcgccgatgg cggcctggat
    27361 gatccccttt tcgtccttga actcgtgaag ggatatctgg ccctcggact tgtatccgat
    27421 gtccaccatg acaaaatcat cgctgatctg gacaattttg ccgcgaatta cctccccttc
    27481 ctgaatattg cggaaactct gttcatacaa atcatgaaga gagtccatgt cttccatttc
    27541 attttcgagt cccgcttcct ccggcgacgt gggctcgtcc ggagcagcct gatggatggt
    27601 gtcttgtgtg gtttcgtctt tttcaaccat tgaatccgtt acccccttgc gcatatttgg
    27661 cacatgccgc tcttgaatcc ggaacagtac caaatcgaat cgtccttgac aacctcattt
    27721 tttctcgctg catcgcgggt tgtccccccc cagcttccgt gaaacgattt cttcaaggcg
    27781 actcaccacc tcttcaatcc ccaggtggga cgtgtccagg atgcaggccc cctcggccgg
    27841 ccgcaacggg gccagctccc gctcttggtc cgccctgtct cgatcttcga tttccttttc
    27901 caacgtccgg aaatcgacgg caactccttt tgcttcgtat tcggcgagcc gcctccgggt
    27961 ccttgcggcg agatcggcgg tgacaaacac cttcactgcg gcatcgggaa agaccacggt
    28021 ggccgtgtca cggccttcgg caaccacgtt tccttgggcc cccaaccgcc tctgccactc
    28081 caccaggaag gttctcactc cttccagctg cgaaaaagcc gacgcaaggt gagacacctc
    28141 cgggcgcctc aattcttccc cgagcctctt gtcctggaag gtgatggtca attggtcccc
    28201 ctcgagggcg aacctcaggg gcaattgatt caaccgggcg caaatctccg agggctcgga
    28261 aatcccgacg ccccgtcgcg tcatcacgta ggcaaccgcc cgatacatcg cccccgtatc
    28321 caggtaggca aaacccagcc tttgggccaa caggcgacac accgtgcttt ttcccgctcc
    28381 ggcggggccg tccactgcaa tgaccattcg ttcactacgt cgctggttga aggtctttca
    28441 acgtctcggc caaggcctcg acgaatcgcc gattctcttc ggggatccct gcattgacgc
    28501 gtatgtaccg gtccagaccg taagcgttca tggcgcgaat gatgacgccg cggcccaaca
    28561 aggcctcgta aagcctcttg gcatccacag gcacccggat gaggaagaaa ttggcctggg
    28621 tcggcagggt ttcgagtccc aggcgtccca cctcttcata aagatagcga agtccgtccc
    28681 aaacggtttc gcgggtccgg aggaggaaag cctcgtcatc cagcgccgcc agggccgccg
    28741 cctgggccag tgttcccgtg ttgaacggtt ggcgcactcg atggaggtaa tctgcaatcc
    28801 ccgggtccat gacgccgtag ccgatgcgca gccccgcaag accgtaggcc ttggagaaag
    28861 ttctcaagac aaggacatga gctccccccc gcggtttgat gtagtcaaac ccccggggga
    28921 tatccgggtc ccgggcaaat tcaatatagg cctcgtccag gaccaccaac atttccggag
    28981 ggagggccct caggaagcga tcccatggct ccttcctcac gacggttccc gtagggttgt
    29041 tgggattgtt gataaagatg atcttcgtac ggggggtgac cgcgcggagg gttgcctcca
    29101 ggtcgatggt gaagtccttg aggggcacgg caaccccctt tccccccatg gtctggaccg
    29161 ccagtttgta gaccaaaaaa gagggggccg gcatgatgac ctcgtcgccg ggaaccagga
    29221 aagcacgaat ggcaagctcg atgatttcgt tggagccgtt gcccagcaca attccttcga
    29281 agggcaggtc cagtttctcg ctcaggcgct tcctgagata atagccgctt ccgtccggat
    29341 atcggtggag tcgtcccaag gtggaacgga tggcttccag cgcccggggc gaaggcccca
    29401 agggattctc gttgctcgcc agcttgatgg agtctcgtat gccgtattcc cgctcgagct
    29461 cctccagggg tttgcccggg ggatagggaa tcaggccggc aatgtgttcg ggcacgaaag
    29521 gtttcatggg gttagatttg tcggcgtgca gaagatctgc accgtaagtc cgagagcctt
    29581 tctacgtggt ttggtattgc tgaattccag agatgtatga tctctataat gaaacccttg
    29641 ggaaaaagca acggatttct gttttccgag gccttccctc ccccttgacc tttccacctt
    29701 gttttccccc gggagtggat tataatggtc cttcatggac cgagacatgg aagacctgat
    29761 tcccggaaga acgcgacccc cgtcgctctt cttttcgctt ccacaaccgc agtatgagga
    29821 ctatgcacgt catgaggcct gtgcacgagc ccaaagattt ggatgaagcc tcgaccaggg
    29881 acggagacac cgactctcga gccccaaaga tatccgtcca tgaaaggcct tcatccgttg
    29941 ccttcgatcc ctttcgcctt tacctcgacg aaatcaagcg gtactccctc ttgagccggg
    30001 aggaagaacg tgaactggcc atacggtacc gtgagaaaaa cgatctggaa gccgcctaca
    30061 agctcatcac cgccaacctg cgcctggtgg tgaaaatcgc catggacttt caacgctact
    30121 ggatgcagaa cttgatggac cttatccagg agggaaacgt cggcctcatg caggccgtga
    30181 agaaattcga tccctaccga ggctacaagt tctcgtacta cgcgtctttc tggatcaagg
    30241 cctacatcat caagttcatc atggacaact ggaagctggt caagatcggc accacccagg
    30301 cccagcgaaa actcttcttc aacctgcgca aggagaagga gcagctggaa tcccagggga
    30361 tcgaggcatc tcccaaactc ttgagccatc gattggatgt gaaagaatcc gaaatcatcg
    30421 aaatggacca gcgcctgaac agctgggaga tttccctgga cagtcccctc aaagaagatt
    30481 ccgaagacac acacaaatcg ttcctgcctt cggacgatct gcccgtagac gaccagatcg
    30541 ccgaccggga agccaaggcc attctgcacg acaagctggt cctctttcgc caacaattga
    30601 agggcaagga agcagtcatt ttcgaccacc gtctgctttc cgaggaaccc atgactctgc
    30661 aggaaatcgg ggatcggttc ggaatcagcc gtgagcgggt gcgccagatc gagagccgct
    30721 tgaagaaaaa gctcaaggct tatttggagg aagaaatcga agacctcgac ctgctccagg
    30781 agagcatgat cgaggtatag gcttcagcac cgctatcttc ccggctcctg tggttctcgc
    30841 ccatttctca tcaggatatt tcctatagaa aaatcaggaa tctcccgatt gactcgcggg
    30901 accgatccgt ggtttctagg agccagactt tttccttctt catcacaaca aggccggggc
    30961 tgtcctccca ctcccccggc ttcttattgt ctgcccggga agtggcgccg ggcccccaac
    31021 acgaatttcc cgcgtgcgga acgcttgccc ccgtcgggcc tcccgtcagc ctgtcttttg
    31081 gcacaccttg gcgttttcct tccagaacgg cgaaaggccc cgaagggtct cgatgatcgg
    31141 agggactttc tccaggacga aatcgacttc ttcctccgta ttgtaaatgc tcaggctgaa
    31201 tcgaatggaa ccatgggcca tggtgtaggg gacccccatg gatcgaagca cgtgggaagg
    31261 ctgcagagac ccggaagtgc atgcggaccc cgaagaagca caaatcccgt attcgttcat
    31321 catgagcagg atcgcctctc cctcgacaaa ttcgaagctg atgttggtcg tgttgggaag
    31381 ccggttgaac cgatctccat tgacccgagt gttgggtaca agggaaagaa tgcggttttc
    31441 cagccggtcc cgcaaccgtc tgacgagggt gttttctatt tcgaggtttt ccgccgccag
    31501 ttcacacgct tttcccaggg cgacgatccc cggcgtgttt tccgtccctc ctctccgccc
    31561 cttctcctgg tgccctccga tcaggaaggg agaaaatttg gtccctctgc gaaggtagag
    31621 ggccccaatt cccttggggg catgaagctt gtggccggaa agggagagca tgtcgatggc
    31681 cgttttgctt aagtcaatgg gaatcttccc caccgcctgc acggcatccg tgtggaagag
    31741 cactcccctt tcccgagcga tttgggccgc ctcgaccacc ggaaagacga cccccgtctc
    31801 gttgttggcc cacatgaggc tcacgatggc cgtgtccggc gtcaggctct tccggtattg
    31861 atccaggtcc aggcgcccct cggaatccac ggggagctcc gtcacccggt aaccccgcaa
    31921 agccaggtcc gcgcacaagg ccttcaccgc agggtgctcc acccgactcg tgacgatgtg
    31981 tcgtttttcg ggagcctgaa gcagagcgga ccgaatcgcc gcattatcgc tctccgtgcc
    32041 gcaactggtg aacacaatct cgtcgggctg agcacccagg agggccgcca cctgctcccg
    32101 ggcttcgcgg acccggacgg ccacctgtcc tccaaaggca tgcatgctgg acgggttccc
    32161 atagaaatcc ttcaggtaag gcagcatcgc ctcgagcact tcgggggcca cccgggtggt
    32221 ggcgttattg tcaaagtata gggttttcac ggggacacct cctcgacgat caactcaggc
    32281 cagacgagtt ctcgaagctt ggattccaca aagtgcttga gagtttgggc ggaggccacg
    32341 caaccggcgc aggctccccg agtggccacc aggacccggt tccccaccac gtccaccaac
    32401 tcgatgtccc cgccgtcctt cttcaacgac ggccggattt ctcgttcgat ggtctcctga
    32461 atcatccgga tcttccgaat gttggaaagg cccggacggg aaggcgcttg gggaatccct
    32521 tcccgaccca gcgttgccag gatttcctcg atggccccgt ggcaactgcc gcaacccccg
    32581 ccggccttgg tatagtgggt aatttcctcc acggttctca gggcgttttc ccgaacggct
    32641 ctctcgatct ccttgtccgt gaccccaaag cattcgcaaa ccacctttcc ttcgggcttt
    32701 tcttcagcgg ttccccgata gtcttcaatg gccttctcca aagcctcacg gcccatgacg
    32761 gaacagtgca tcttctccag gggaagtccc cccagataat cggcgatgtc ctggttggta
    32821 atccgggaag cctcctcgac gctcatccct ttcaccattt ccgtgagggc ggaggcggcg
    32881 gcaatggcac tggcgcagcc gaaagttttg aactttgctt ccacgatccg cttgttctcg
    32941 tccagccgaa agctgaaacg aagagcatcc ccgcacgcga tggaaccgac ttccgccact
    33001 ccgtcggcct cttccacctc gcccacgttt cgggggtgca ggaagtggtc tttgactttc
    33061 tccgtgtatt cccacatgag cttcactcct tgccgtacag tcctgcctcg ggaggggcgg
    33121 actttccccc cttccgtgag tccctttgtc tagatgagcc ttgcggtggc cggcctacga
    33181 ccgaaacctc tgctctccaa gatcccggat ccgttccccg ggggggccga ccctcatcct
    33241 tcggtccttc aggagcaaag ctccgatcgg cccccagatc cgggtgtttc ctcggagaca
    33301 agccgaatcg cgatttgtct tcccaccaat ctaacaccgg cggtgtgccg tggctagtcc
    33361 gcgcccggat ctgcggggag gcggccggac gggggcattg cttgtgccgg gcacgagccc
    33421 ttcggggaaa accgcctgcc ctcctggtat ttcgtccaaa ccgggcaatc cctatcgggg
    33481 ggggccatac taccggtcat atccgtcatg gcacgggact tgccaatcat agcgggggcc
    33541 gtacgggttg cttgcccccg gaccgagcga tccttgatgg tcattccccg tccaacggca
    33601 ctctcggagg gcggggacag ggcttcgccc cttggatgaa ccgttctgcg ggctcggggg
    33661 tgggctttat gcacacccat acaaggggaa aaatcgccca tcccgagtgc ccgtcggaac
    33721 cgccgcggac cggcaccggt cgaacggaac gcgatttgat tttacacccg atttgggata
    33781 gaatttaaaa tcacgtaccc acggaacggc tctcggaggc gactggattt cacgcgaatg
    33841 cgggaaaggt gcggaatatg gaatggtacg ccatgcagtt ttgtgtgacc cctgctcagg
    33901 atgtcagttt ctttggagcg atgcaactca tcattctggc cgccatctgg gtgtttggct
    33961 tccgcttccc caagcccacg aaggaagatt gaacccgaag agtccgaaac ggattgtcga
    34021 gcagccggtc aatttttcgt ctgatccgcc tgcagcgacc cgtttcatgg accgaaccgc
    34081 tggacgttca tgactgagcc ctgcccatcc gccaagtcgg agaccgagaa cggggagacg
    34141 cccccgacca ccggtgcgtt ctcatcggag cgaactgccg atcacaagcc ctcggggacg
    34201 ggtgcctttt cgagatctcg ggccatggtt cagtgccttc atctttacaa gtcccatccg
    34261 ggaacgggac ccatccttca aaacgcctgc ctggaaattc gaaaaggcga atgggcc
//
LOCUS       DAOOVC010000028        32888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_898_length_32888_cov_6.688850, whole genome shotgun sequence.
ACCESSION   DAOOVC010000028 DAOOVC010000000
VERSION     DAOOVC010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 32888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32888
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_32888_cov_6.688850"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLM79_05795"
     CDS             complement(<1..590)
                     /locus_tag="PLM79_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85854.1"
                     /translation="MKTCKSTLAVDSLPSAPGNSFLFHRAFMAMALGNFFVGSSFNSF
                     AIFPLFVGEHGGNRVDVGILMGAVMFSSVACRPWISSMIDRMGRKRGFAIGALIMSVL
                     PLVYLLFRGDLSSFFWLLLPVRLLHGIGLALCITAAFTYAGDIIPEGRLNEGIGIFGI
                     SGLIGMALGPVIGELVSVHLGFSAFFLVAAGLGTVG"
     gene            complement(968..1390)
                     /locus_tag="PLM79_05800"
     CDS             complement(968..1390)
                     /locus_tag="PLM79_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85855.1"
                     /translation="MRNKPSLHQKIQALCDCFAAADPLKEMSVVANDSDQEEAALKWV
                     APAILHGIENNASKISISVSEKGGAEVSAQYREARLPSPGKEIGGRVLGSVRELFRSE
                     AEKFKMPLAVGVRDSSLRIDVKVKKENGVETLTMKFPE"
     gene            1685..3112
                     /locus_tag="PLM79_05805"
     CDS             1685..3112
                     /locus_tag="PLM79_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HOV85856.1"
                     /translation="MKPHRKAVLVFLRHPEAGQVKKRLAATIGAEKALHVYLKTARRT
                     LGLLCDLKEEQPGIDLFVFFTPCEKEAEVRNLFSGPWEFVGQKGTHLGLRMENAFGCA
                     FARGYEQVLLVGSDIIDLEIQDFLDGFSLLEDHPSVLGPACDGGFYLIGLRTFCRAPF
                     SPSQWGGEKVFSRTEDLLARSGFAPGLLPRRTDADRSRDLPVFYRDPMNSCMLSVIIP
                     TLRPLRHLSPLLSRLERLLWPGDEIVVALGSSSPPGSADIWNERTRAVSCPTGRGIQL
                     NHGAAAARGDLLLFLHEDSVVPSHLAYLVRKLCREPGYSLGCFRLGFHPSSSFLDWIA
                     RWANFRTRFFHLPYGDQGFFCRRETFDRVGGFRKPFLMEDVDFVRRCRRIGPILVLRE
                     KIRTSPRRYLAGGVLATALQNHLILLLHQLGVSDEKLYSLYYGRKPTPYDLPREGALG
                     HGRDALPPPGHRVPVRPGKESGGEG"
     gene            complement(3129..3833)
                     /locus_tag="PLM79_05810"
     CDS             complement(3129..3833)
                     /locus_tag="PLM79_05810"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOV85857.1"
                     /translation="MIPLRDVNPSRGTPVVTYVLIALCLGAFLYELSRGRDLHEFLLR
                     YGLVPAHYFQAEIRHHLTLWEQALPFLTSMFLHGGWLHLIGNMWVLRIFGDNVEGELG
                     HGKYLLFYLCSGGCAAFIQLVTDPGSSIPTIGASGAVAGVMGAYFVLYPRAKILTFVP
                     ILVFFTLAELPAYVFLGFWFLLQFFSGTLSLMADGRGFSNVAWWAHIGGFLGGIVLLK
                     LFHRRPLPPEHFDEFD"
     gene            complement(3867..4286)
                     /locus_tag="PLM79_05815"
     CDS             complement(3867..4286)
                     /locus_tag="PLM79_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV85858.1"
                     /translation="MFKHILIPTDLTDKSLRALEIAAKIAVHDQARLTLLHVIETIED
                     AFEGELDDFYRKLGRRAHKKMDQFLKLHETKEIRIDKEIIYGKRVKEIIRFAGDRDID
                     LIILSSHKVEIDNPGLGWGTISYKVGILAHCPVMLVK"
     gene            complement(4618..5157)
                     /locus_tag="PLM79_05820"
     CDS             complement(4618..5157)
                     /locus_tag="PLM79_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV85859.1"
                     /translation="MEAMETILSRRSVRSYLPRPVPREAIRKLLEGAMSAPSAGNQQP
                     WHFLVIDDRKILDAMPSVHPYSQMLREAPLAILICGDLQLEKHKGFWVQDCSAAAQNL
                     LLAAHAQNLGAVWLGVYPREDRVSGFQKMFGLPRNLVPFCIVSIGYPAEKIPRAHRYN
                     ASRVYHNEFARGWEGDHSG"
     gene            5505..6947
                     /gene="gatB"
                     /locus_tag="PLM79_05825"
     CDS             5505..6947
                     /gene="gatB"
                     /locus_tag="PLM79_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOV85860.1"
                     /translation="MEFEPVIGLEIHAQLLSKSKIFCGCSTTFGAGPNTHTCPVCLGM
                     PGVLPVLNREVVEYAMRMALATHCRIAPVSRFARKNYFYPDLPKGYQISQYELPLARD
                     GWVRIETENGPRKVRIHRIHLEEDAGKLVHDEHQPFSYVDFNRTGVPLIEIVSEPDLR
                     SPDEAASYLKTLRDILRYLEICDGNMEEGSLRCDANVSLRPSGTTPLGVKTELKNMNS
                     FRNVQRALEFEIRRQRALLERGQSIVQETLLWDANRNVTVSMRGKEEAHDYRYFPDPD
                     LVPVEIDSGWIEEVRAQLPELPDAKRERFMKQYALPEYDAQVLTSQKALARYFEQVIE
                     TFPQPKAVSNWIMSELLRELKKEEREIETFPVSPDRLGELLGLIASGVVSGKIAKTVF
                     EQMHTTGKSARQVVDEQGLVQVTDQDAIEALVDEVLSENPREVEQYRGGKEKLMAFFV
                     GQVMRKSRGKANPQAVNEILRKKLARKESP"
     gene            7059..8108
                     /gene="mtnA"
                     /locus_tag="PLM79_05830"
     CDS             7059..8108
                     /gene="mtnA"
                     /locus_tag="PLM79_05830"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOV85861.1"
                     /translation="MPEKWMPPIYWEGDVVVILDQRLLPHREVTLRCTTARQVIQAIR
                     NMAIRGAPAVGIAGAMALALGAKSIREEDPRAFVRKFFHLCRQVKAARPTGNNLGWAV
                     EKIYSIVVENPQAGVEDMRRLIRKKSQEILEEDVSSNLAMGAWGKQVMPQGARILTYC
                     NAGALATGDYGTALGVIRAAFAADPDIQVFSCETRPFLQGSRLTAYELKQAGIPVTLI
                     TDSAVGTLMQQNRIDVVVVGADRIAANGDTANKIGTYTVAVLAHAHNVPFYVAAPRST
                     IDPTLSHGSQIPIEQRDPKEVTHFLGRRVAPDGVPALNPAFDVTPHRYISGIITEVGI
                     LRKPFGKAIRKALET"
     gene            complement(8142..9512)
                     /gene="radA"
                     /locus_tag="PLM79_05835"
     CDS             complement(8142..9512)
                     /gene="radA"
                     /locus_tag="PLM79_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOV85862.1"
                     /translation="MAEKRIVYRCQQCGYLSPKWLGRCPECQTWDGMVEERSAKGSSR
                     APATPSVKSRPVPFHEISSQDRARIVTGIHEWDRTLGSGLMPDSMVLLAGDPGIGKST
                     LLLQALARMARDRQVLYVSGEESPHQLKNRAERLGVRSERLLAYSECSLESVIEVIET
                     LAPEILAVDSIQTLYSEALDVAPGSISQVRECAVRLMRVAKEKGISVILVGHVTKEGA
                     IAGPKALEHLVDTVLYLEGDRTHAFRLLRSVKNRYGSTNEIGVFEMKEEGLEEVPNPS
                     RLLLAERPVGVSGSVVVCSVEGTRPLLVELQALVSATGWVQPRRTTMGVDVNRLSLLV
                     AVLEKKVGLSFAQQDVFMNVAGGIRIIEPAADLAVVTALISSYLDRPVPPDLVVWGEV
                     GLAGEVRGVGHSVPRLAEAHALGFRKHLVPAGNVERLSRELGANCLGVRSLQEVMDAL
                     FNSPGR"
     gene            9846..10175
                     /gene="trxA"
                     /locus_tag="PLM79_05840"
     CDS             9846..10175
                     /gene="trxA"
                     /locus_tag="PLM79_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOV85863.1"
                     /translation="MAGNIIEISDSSFEKEVLQSDVPVLVDFWAPWCGPCRAIAPVIE
                     ELSGEYSGKLKVAKCNVDDNPKTPSRFGIRAIPTLILFKGGSVSEQITGAVAKSQIAS
                     AIDKVVS"
     gene            10251..11180
                     /gene="trxB"
                     /locus_tag="PLM79_05845"
     CDS             10251..11180
                     /gene="trxB"
                     /locus_tag="PLM79_05845"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOV85864.1"
                     /translation="MNDLFDIAIIGGGPAGLTAGLYAARARLKVILLERMGFGGQLLT
                     YEKVDNYPGFPEGISAFGLSELISAQALRFGLLTRNAEVTGLEFEAPIKRVVLADGFI
                     EAKTLILASGASPNKLGVKGETEFTGRGVSYCAVCDAPFYRNQEVAVVGGGDTAIEEA
                     VYLTKFAGRVHVIHRRDRLRATQIIQEKAFQNDKIDFILSTVVKEIRGGSGGVEGLIL
                     EEVPGGRTSELAVSGVFVFVGIHPSTDYVPASLERDPLGFIRTNQEMATSVPGVFAAG
                     DVRSKELRQIVTAVGDGATAAFNAGRYLEAHSL"
     gene            11208..11975
                     /locus_tag="PLM79_05850"
     CDS             11208..11975
                     /locus_tag="PLM79_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOV85865.1"
                     /translation="MSPSFPMIQLQRFACALILAGVVLVTGGCGTNLVQFYFGDLFGK
                     TSSTVNRNAAQLAQEGMQKMRQKKYGDALKDFKQLKEHYPYSKYAILAELKIGDAHFQ
                     RKEYSEAAIAYEEFARLHPRNEVVPYVLYQIGMCHFLSFSTVDRDQEETRLAVESLQR
                     VVQAYPDSDYAQKAKKQLNECKKRIVAHDYQVGRFYYRQGEYRAAKERLQKAVMTHPD
                     AVKELGYLDSVNKMLAVCEKEISKGEKKPSIWTRLGF"
     gene            12435..13682
                     /gene="rho"
                     /locus_tag="PLM79_05855"
     CDS             12435..13682
                     /gene="rho"
                     /locus_tag="PLM79_05855"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOV85866.1"
                     /translation="MNLVELKGRNIRELLGTAKALNIEGASSMRKQELIFAILQAQAE
                     MSGLIYGEGVLEILPDGFGFLRSQNYNYLPGPDDIYVSPSQIRRFSLRTGDSISGQIR
                     PPKDNERYFALLKVEAVNYEDPDVARDKILFDNLTPLYPDRRIRLETVADNYSTRVMD
                     LLTPIGLGQRGLIVAQPRTGKTMLLQNIANAISINHKDGYLIVLLIDERPEEVTDMQR
                     NVRAEVVSSTFDEPPQRHVQVAEMVIEKAKRLVEHKRDVIILLDSITRLARAYNSVVP
                     PSGKILSGGLDSNALHRPKRFFGSARNVEQGGSLTIIATALIDTGSRMDEVIFEEFKG
                     TGNLEIVLDRKLADRRVFPAIDINKSGTRKEELLISPDDLNRIWILRKLLSPLNPIDA
                     MEFLLDKMQGTKHNADFLASMNR"
     gene            13913..14143
                     /gene="rpmE"
                     /locus_tag="PLM79_05860"
     CDS             13913..14143
                     /gene="rpmE"
                     /locus_tag="PLM79_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOV85867.1"
                     /translation="MKDNIHPQYHETVIRCACGNEIPTGSTKKEIRVEICSKCHPFYT
                     GKQKLVDSAGRIERFLKKYEKYQTDSSSGSKG"
     gene            14355..15425
                     /gene="prfA"
                     /locus_tag="PLM79_05865"
     CDS             14355..15425
                     /gene="prfA"
                     /locus_tag="PLM79_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOV85868.1"
                     /translation="MFDRLQSVEEKYRKLEEDLSNPEMLANQKEYQRVAKERSDVAPL
                     VEVYRQYKALKQELQDNHELLNEEPDPEMRELIRQEISDLRQKLQAVEDELKFMLAPK
                     DPNDEKNVILEIRAGTGGEEAALFAADLFRMYTRYAEIRRWKVEVLDSHPTGIGGFKE
                     IIAAINGKGAYSRLKYESGVHRVQRVPLTESQGRVHTSAVTVAVLPEAEEVDVYIDPN
                     DLRVDVFRSSGPGGQSVNTTDSAVRITHLPSGLVVICQDEKSQHKNKAKALKVLRARL
                     LDQMQREQEAKIAQERKSQVGTGDRSERIRTYNFPQNRVSDHRVNLTLYRLDAVLNGE
                     LDEIIDPLAAHSHAEALQRMDS"
     gene            15523..16395
                     /gene="prmC"
                     /locus_tag="PLM79_05870"
     CDS             15523..16395
                     /gene="prmC"
                     /locus_tag="PLM79_05870"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOV85869.1"
                     /translation="MEESWTILKVLQWTTGYFGRKGIEQPRANAEVLLAFVLGMERID
                     LYLRHDQPLTASELSRYREVVRRRALREPTQYITRRQEFWSLEFEVDPAVLIPRPETE
                     LLVEKALDLVGSDPKKVLEIGTGSGAISVALAHECGSVQILATDKSPEALRVARRNAR
                     RHGVLERISFVAADLFEGLHPGNARFHLVVGNPPYVSDEEYADLPEEISGHEPAMALL
                     GKGPRGLDVVRRILQTAGAFLTAPGDLLLEIGRGQAEILEQEPKLFPGLRLEGFLRDH
                     SGILRIMHLRKVIG"
     gene            16399..17652
                     /gene="murA"
                     /locus_tag="PLM79_05875"
     CDS             16399..17652
                     /gene="murA"
                     /locus_tag="PLM79_05875"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOV85870.1"
                     /translation="MDKLVIEGGLPLEGEVPVSGAKNAALALMAATLIAGGKHRLDHV
                     PRLRDIRTMELLLEHMGAVCHHNDHLVIDTGNIHCLEAPYDLVKTMRASVMVLGPMVA
                     RFRRARVSLPGGCAIGARPINLHLKGLEQLGVGIELDHGYVVAKADRLRGAVIPFDQV
                     TVTGTENLMMAATLAEGVTVLRNAAREPEVVALAEYLRRMGAQIRGEGTSEIVIEGVK
                     ELVPASFRVIPDRIEAGTYLVAAGITGGNLKVTGCRPEHLDAVMGKLELAGMTFQVSG
                     DSIQATGTGQIRSINLKTWPFPGFPTDMQAQFMALMCLGNGVSLITEQIFENRFMHVL
                     ELKRLGADIELDGRSAVVKGVRGLSGAPVMATDLRASASLVLAGLAAEGITEVNRIYH
                     LDRGYEAIEKKLSAVGARIWRERGV"
     gene            17684..20236
                     /locus_tag="PLM79_05880"
     CDS             17684..20236
                     /locus_tag="PLM79_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOV85871.1"
                     /translation="MPEPPAFGPAGEGAPGPRPWPRPLLLLAAALAAGIALESQVPEM
                     FPEPWVWAAPALLFLLSLLGFFPACRRLRSPPTAVILFFALGAALSRQADPPLPSPPS
                     LQSFLKQGHTLFLAEIDRPPEFPPNRTRLRLRLHKAIPDGGSVPVEGKVLLTIGQARQ
                     TPGRYLLGDILLVRLNLRPLHNFNNPGGFDYVRAMAEQGVFARAYLADDRLVLPVTGE
                     PLSIRRLPALMGRSLDRFRQNALFWLRAHLDRDEADFYAALLLGYRIPDEWGDHLSRT
                     GLTHLLSISGLHLGMVFLASFWLVRRLVRRTLPNVLRKRDDQSIALWFSLGAAAVYAL
                     ISGMALPTWRSLFMLILFFLAASRYRRADSLTGLALAALLILALSPKALGQISFQLSF
                     AALFGLFCIYPHLDRLRQFLGRRAAFPEMPGRTLIKLICDAFWVSLAANVMVLPIVAY
                     HFHGISPAGFLANALLVPWTGFVVLPLGLLSLAVFALWPPLGLPFLLLGGWCLGITLP
                     AIRWFSSLSWAFFWVGAVPLWGLAGYYAAVAVLLCPWSGKTRVAALAALLLWVVGIQQ
                     AFRLSAHPNSSSLLRVTFLDVGQGSSTLLQFPDGRVMIVDGGGFWDNSFDVGRFVVAP
                     ALWHLGIRKIDWMVLSHDHPDHRNGLRFLLSHFSVGEFWESGIHDKGAELEPLSTVAF
                     RRAIRVRSFTELAGAHHFGPCRVRLLHPSPHYLREEWDRKDLNNLSAVLRVDFGGTHL
                     VLPGDIDRSIEERILGGEDLPGQVLLAAPHHGSERSGSPLLFDRLRPRAVVFSCGADN
                     WFGFPSSAVLQECTRRNIRIFRTDLQGAVRARSDGVRWTLTPHLEGKQPGGS"
     gene            20280..21137
                     /locus_tag="PLM79_05885"
     CDS             20280..21137
                     /locus_tag="PLM79_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV85872.1"
                     /translation="MRTYFFGDLHGNAEALEACLRHMDRVRPDAALCLGDLVGWLPFG
                     DRTLTRMRELDLPTVAGNHDLLVAGALTDFPEQIDRMQASAYNAGLLSTVPGAVEYLL
                     SLPLSLEEKDFAVVHHSPFHLPSPGRVPTIHSFHYLDSRALGESLDLWRGYSRRLIFS
                     GHDHDPAVFELSDGAEPRLENVKVYSPPPSGNLTISLKSGARYWVKGGSVGGPYRDGI
                     PAANSVLYDSEAETVTLFRVPYPTRRLMEELTSHPFHRNLPTMKKYIELLSSTATDLS
                     GPEPPREPS"
     gene            complement(21094..22038)
                     /gene="galE"
                     /locus_tag="PLM79_05890"
     CDS             complement(21094..22038)
                     /gene="galE"
                     /locus_tag="PLM79_05890"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOV85873.1"
                     /translation="MKILVTGGAGYIGSHTAKRLKQVGHTPIAFDNLSNGWPEWVKFG
                     PFAFGDIRSEEALFRALSGFQADAVIHFAAKAYVEESTRIPEEYFDNNVGGTVALLKA
                     MKRAGTKYLVFSSSCATYGNAVTPTIQEDHPQEPTNPYGTSKLLCEQVIRAVAPVVGI
                     RFAALRYFNVIGNDPDGEIYEKHEPETHVLPNLMKAARTGAPFQLFGTNHPTPDGTAI
                     RDYVYVMDLAEAHIKALDVIRSRERLISNVGSGSGTSVRELVSTVENALGTRVNVDEK
                     PIRPGDPPRLVADDAFLRTWFRHTFKTVPEVVRALTNP"
     gene            23055..24044
                     /locus_tag="PLM79_05895"
     CDS             23055..24044
                     /locus_tag="PLM79_05895"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOV85874.1"
                     /translation="MSGTTHRWCKELLTVCRVLPLLVLAASCSPSASPDKVQGYVEGE
                     FVYVSSPLSGTLKELQVQRGDRVGAGDPLFSLDDTPEKAALEEALGRLAQARANLEDA
                     RKGKRPSEIESLEAQIGQARAALALSEKELARQEKLMRIPGATTEQDLDRARAARDQD
                     ARRTAQLEADLKTARLGTRTDLVLAAQAAQQAQEAVLARAQWDLSQKAQSAPDSGLVF
                     DTLYRVGEWVAAGRPIVSLLPPQNIKVRAFVPETKIGAVRLGDPVRVGVDGIKEPLIG
                     RVSFISPRAEFTPPVIYSLESRGKLVFMVEAVFDPQTAVKLHPGQPVDVRFGS"
     gene            24048..24974
                     /locus_tag="PLM79_05900"
     CDS             24048..24974
                     /locus_tag="PLM79_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85875.1"
                     /translation="MTDELSIDVHGMTKRFGSLTAVNRIDLEVRKGEICGFLGPNGSG
                     KTTFIRMLCGLLRPDEGSGTCLGLDVIRQSEAIKRQVGYMTQRFSFYEDLSIEENLDF
                     VARMYSMNDRRRAVNESLERLGLSTRRNQLAGKLSGGWKQRLALAACMIHHPKLLLLD
                     EPTAGVDPKARREFWDEIHRLAGEGLTFLIATHYMDEAERCHRLAFILYGNLLVHGTA
                     SEVIAKSRLTSWTVTGPDLARLSERLRQLPGVEQVAAFGNTLHVSGGDADRLEEAVAP
                     FRIPPYQWQRTTSGLEDVFIHLMDGSNGGRSA"
     gene            24971..26113
                     /locus_tag="PLM79_05905"
     CDS             24971..26113
                     /locus_tag="PLM79_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV85876.1"
                     /translation="MRKRRGFSLGRFWGIVVKEFIQMRRDRLTFGMMIGIPLLQLILF
                     SFAINSDPKNLPTALLQADHGPQARTLLHAIRNSAYFNFVMELQSEEEARDVLAQGRV
                     QFVVHIPENFSRDFLRGDRPAVLVEADATDPAATGNAVNSLANALNSGLKNDLKGPLA
                     YLAPCDSPVDLRIHPRYNPEAITQYNIVPGLLGVILTMTMVMITGLAITRERERGTME
                     NLLSMPARPFEVLLGKILPYILVGYIQVALILLVAHYVFRIPMVGSVPLLLLVSIVFI
                     AANLAMGITFSTVAENQLQAVQMAFFFFLPSLLLSGFMFPFRGMPVWAQHIGEVFPLT
                     HFLRIVRGILLKGNDFNDIAVDLWQIVLFAVVALVIGVKRYRQTLD"
     gene            26223..27656
                     /locus_tag="PLM79_05910"
     CDS             26223..27656
                     /locus_tag="PLM79_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOV85877.1"
                     /translation="MPRLKPIQALGHITRLAVALLILTTCAVGPDYRKPPVEDITPAD
                     WRWKIAEPRDTISRGEWWTVFGDPVLAALEASAVAANQDLRAAVARVDEARSAARIDR
                     SEFFPEISLDPSVKRERTSGHMPTPIPIPIPASHVNTYSVPFDLSYEVDVWGRVRRSF
                     EAAQAQAEASVSDYWSVLLTLTADVAANYFHVRALDSEIAVLQQTIQSREEMVRVLEG
                     RFLAGAVPEIDVARARTELAGARADLADARRQRAETLHALALLCGKPAGDFELAERPL
                     AGSPPQVPPGLPSSLLERRPDIARAERTLAAKNAQIGVAKAAYFPVISLTGQAGYLSA
                     EVNELFADSTRVWSISPGLSLPLFTGGRTQARVRQAEAIFRESVAAYRQTILTAFKEV
                     EDSLAQLVLRGEESVAQMEAVTAAQRSVELARARYEAGNGSYLDLLDADRTRLQQERK
                     LAQATGLRFEAAVHLIKALGGGWEGGT"
     gene            27881..29032
                     /locus_tag="PLM79_05915"
     CDS             27881..29032
                     /locus_tag="PLM79_05915"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HOV85878.1"
                     /translation="MFQKAVFPGKYIQGVGAMSELPALIALLGKQGLVLASPTSKDKV
                     LPRCGIDPDGSRLAIEPFRGECCEEEVRRVSAVLDRSHADVLVGMGGGKTIDTAKIVA
                     DRAGIPVIVIPTIASTDAPCSGCAVLYSPDGAFHSVCYQKANPAVVLVDVSIIAAAPA
                     RFLVAGMGDALATWFEARSCHRTQSPNECGGLSTSVGLHIARLCYETLLSYGPAARLA
                     AEKHVVTPALEHVVEANILLSGIGFESSGLAAAHSIHNGLTALAETHSFYHGEKVAFG
                     VLAGLQLTDASKEESDTVYSFCEEVGLPTTLRDIGLENGDREKLRTAAEKACAPREAV
                     HHEAGTITPEKVLDAMLAADALGMRRGGRGNARRRGRFEGRMNRRSENV"
     gene            29261..29746
                     /locus_tag="PLM79_05920"
     CDS             29261..29746
                     /locus_tag="PLM79_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOV85879.1"
                     /translation="MTIGGWFLFLWLVYFLLPFDILHDRIPYLGRADDLLLLLYLMWL
                     YWKKNRTASKERGSTSSDDGTFSGRERAEQQGAGHQRAQGHAPGQESRARPRDPYRIL
                     GIDPSASTEEIKTAYRQQAARYHPDKVSHLGEEFQKLAKEKFQDIQWAYEQLMNRRRK
                     A"
     gene            29764..30645
                     /locus_tag="PLM79_05925"
     CDS             29764..30645
                     /locus_tag="PLM79_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOV85880.1"
                     /translation="MTDDQSFVSIHTLTNRFEGDLLMQALAGEEIPTILRSFEETPYD
                     GLFIPQRGWGQLLVPEPLASRARRIIQPLLDHLESRGPYSNPADIDPQLWESLRRADP
                     DLVCRNAEVGYDAERGVYRVPFLSRELHCFPGEERIEPREPLPCNLQDFQSYLVVLHY
                     LLEARPASLVGKWISEREIPGGELFFRGPHRFPTGSLVRCFGSRKDLFREVSQRLQGV
                     PVSMGDVAFRYSVLPRIPLVLVLWEGDDEFEPAFHFRFDAGVSRQVKSLDTLWAMVNV
                     VSSCLESLARELTESTE"
     gene            30650..31417
                     /locus_tag="PLM79_05930"
     CDS             30650..31417
                     /locus_tag="PLM79_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOV85881.1"
                     /translation="MRRPFRVISLAPTQTEIIAAVGGLELLEGVTENCDFPEDVRWVP
                     VFGSWHAPDLRRVLDAQPDLVCTFGKHQEELSMALEESGLRVYHSDPGDVPSSLKTIG
                     EIGSILGCDQAARRLIDALETRLDRVERALGRRSPEERPRVLRIMNWDPLITVGPGSF
                     QHDVIERAGGRNLMEDGLQPYFACDPERVLDRDPEVIFFCEPHIREVLEADPAWRRCS
                     AVRDSKIFIFDCGLTCRSGPRIVDMVEQLARALHPGP"
     gene            31538..>32888
                     /locus_tag="PLM79_05935"
     CDS             31538..>32888
                     /locus_tag="PLM79_05935"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOV85882.1"
                     /translation="MNHPRFRSIGRRPRPFAFCTAIVTILLVSSGWAVPARAEVKPIP
                     EDLTTLTLEDLLGIEVTSASKKLQRVSDAPAAIFVITQDDIRTSGATSIPELLRMVPG
                     IQVARLDSNKWAVTSRGFEGRFANKLLVLFDGRTVYTPLFSGVFWEDQDYLLEDIDRI
                     EVIRGPGASLWGANAVNGVINIITKHSKDTQGALISSGAGTYEQGFVGVRYGGRLSPN
                     LTYRLYGKYFNRGTFDDEWGMQGADDWWAYRSGFRLDYDKPGKDALTLQGDLYHLDSG
                     SRYLVPAFTAPYADLFDHTSNHPGGNVLFRWSHTFSPSSQVSLITYYDRAQVKDVILS
                     NVTDTFDIDFQHTLKLAPWNELIWGLGYRYYRDNIDNSEVVTFDPSHTRNQLLSAFIQ
                     DEFKFLDDHLRLTLGSRFEHNDYTGFEAQPTARLLWAPNPDHSVWTAFSRAVRTPSQV
                     "
BASE COUNT         6369 a        10345 c         9933 g         6241 t
ORIGIN      
        1 aaccctacgg tccccagccc cgccgccacc agaaagaatg ccgaaaaccc caagtggacg
       61 gagacaagct ccccgataac gggtcccagg gccattccga tcaggccgga gatgccgaaa
      121 atcccgatcc cttcgttcag tcggccctcc ggaatgatgt ccccggcgta ggtgaaggct
      181 gcggtgatgc acagggccag gccgatcccg tgcagcaacc gcaccggcaa cagcagccaa
      241 aagaaagagg acaggtcgcc ccggaacaga agatagacca ggggaagcac gctcatgatg
      301 agcgccccga tcgcaaaccc tcttttcctc cccatgcggt cgatcatgga ggagatccac
      361 gggcggcagg ccaccgacga gaacatgacc gctcccatga gaatgcccac atccacacga
      421 tttcccccgt gttctcccac gaacagggga aagatggcaa aagagttgaa gctcgagccc
      481 acaaagaaat tgcccagggc catggccatg aaggcacgat ggaagagaaa ggaattcccc
      541 ggagcggatg gaagggagtc tacggccaag gtgctcttac aggtcttcat gaagggttct
      601 gcgcaagggg tgcatgaaat ccacgggatt gcctgaacga tgatctcctc tacgtcgggt
      661 gttgcctcca tcggtctgca gacaccgggg cgttcggaga cggaaatagg gttcctcgga
      721 tccccccgcc cacagatcga aacccgagtc gccgcgacgg gagggtgacg ggcaggtccg
      781 cccccggttt ccaagatccc atgctttggc cattgattcc ccccgcattt tcccgaagga
      841 gcccggcgga aacccggaag gcgacactct gccgcaatac agtaccatct tccgtgccga
      901 cccgccagag caaccctcgc tttttgacgc gtcggcacag gtcggatggg aatgccccca
      961 acaggcgtca ttcgggaaat ttcatggtca atgtctcgac cccgttttct ttcttcacct
     1021 tcacgtcgat ccggaggctg ctgtctcgga cccccacggc caggggcatc ttgaacttct
     1081 cagcctccga acggaacagc tcccgaacgg acccgaggac ccttcccccg atctctttgc
     1141 ccggtgaagg caggcgtgct tctcgatact gggcggagac ttccgcgccc cctttctcgg
     1201 agacggagat ggaaatcttc gaggcgttgt tctcgatccc atgcaggatt gccggggcaa
     1261 cccacttgag tgccgcctct tcctggtcgg agtcgttggc gaccacggac atttccttga
     1321 gcggatccgc cgcggcaaaa cagtcgcaca atgcctgaat cttctggtga aggcttggtt
     1381 tgtttctcat gagatgtccc tcccgtctga aacttcacga gtcgtccctc gggacgttcg
     1441 gggatttcct cgcgaccctg aggcctccac ccgtcctgca ggccccacga tcttgcggta
     1501 tcggatgttc tgttacctta cagaataaga tctccccccc ttgaagcaag caagcgggga
     1561 agatcccgta aaagccgaca aattccttgt ccccggtctc caaagggggc aatatttctt
     1621 cccgggcgga atggttcggg gaggtttcgc tcccggagag tttcttctcg gttttggacg
     1681 gttcatgaaa ccccatcgga aagcggtcct ggtctttttg cgtcacccgg aagccggaca
     1741 ggtcaagaag cggctggcgg caaccatcgg cgccgagaag gccttgcacg tttacctgaa
     1801 gaccgctcgc aggaccttgg ggctcctgtg cgacctcaaa gaggaacagc cgggcatcga
     1861 cctttttgtc tttttcaccc cttgtgagaa ggaggcggag gtccggaacc tgttttcggg
     1921 tccctgggaa ttcgtgggcc agaagggcac ccacctgggc ctgcgaatgg agaatgcctt
     1981 cggctgcgcc ttcgcccggg gctatgaaca ggttctcctg gtgggctcgg acatcatcga
     2041 cctcgaaatc caggattttc tcgacgggtt ttcgctcctc gaggaccatc cctcggtgct
     2101 gggaccggcc tgcgacggag gcttttacct catcggcctc cggacattct gccgggcgcc
     2161 cttttccccg tctcaatggg gcggcgaaaa ggtcttttcc cgaacggagg acctcctggc
     2221 acgatccggg tttgctccgg gcctgctgcc ccggaggacg gacgcggacc gatcccggga
     2281 cctcccggtt ttctaccggg accccatgaa ctcctgcatg ctttccgtaa tcatccccac
     2341 gcttcgccct ctccggcacc tttcgcccct cctgtcccgg ttggaacgtc tcctttggcc
     2401 gggagacgaa atcgtggtgg cactggggtc ctcgtcccct ccggggtccg cggatatatg
     2461 gaacgaaagg acccgagcgg tatcctgccc cacgggaagg ggaattcagc tcaaccacgg
     2521 cgccgccgcc gctcgagggg atcttctcct ctttctccac gaagacagcg tcgtcccttc
     2581 ccacctggcc tacctcgtcc gtaagctgtg ccgagaaccc gggtacagcc tgggatgttt
     2641 tcggctgggg tttcatcctt cctcgtcttt cctggactgg attgcccggt gggcgaactt
     2701 tcgaactcgg tttttccatc taccctacgg ggaccaggga tttttctgcc gcagggagac
     2761 cttcgatcgg gtggggggat ttcgcaagcc cttcttgatg gaggacgtgg atttcgtccg
     2821 caggtgcagg cggatcggtc ccatcctggt gcttcgagaa aagatccgga cgtccccccg
     2881 aaggtacctg gccggggggg tcctcgccac ggcccttcaa aaccacctca tcctgctcct
     2941 gcaccagttg ggcgtaagcg acgagaaact ctattccctt tactacggca ggaaaccgac
     3001 accttacgac ctgccccggg agggggcctt gggacatgga agggacgctc ttccgcctcc
     3061 cgggcatcga gtgcccgtcc gtcccgggaa ggaatccggg ggcgagggat gatcctcgag
     3121 gccggactct agtcgaattc gtcgaagtgt tcgggcggca aaggacgccg gtggaagagc
     3181 ttgagcagga cgatgcctcc caagaatccc ccgatatgag cccaccacgc cacattgctg
     3241 aagccgcggc cgtcggccat gagggaaagg gttccactga aaaactgcaa caggaaccag
     3301 aagcccagga agacgtaggc gggaagctct gccagggtga agaaaaccag gatgggaacg
     3361 aaggtcagga tcttggctcg cggatagagt acgaaataag cgcccatgac ccccgcgacg
     3421 gccccgctgg caccaatggt gggaatggaa gagccgggat ccgtcaccag ctgaatgaat
     3481 gctgcgcagc cccccgaaca gaggtaaaag agcaggtatt tcccgtgccc caattctcct
     3541 tccacgttgt ccccaaagat tcgcagaacc cacatgttcc cgatgaggtg cagccagcct
     3601 ccgtggagaa acatggaagt cagaaaggga agggcctgtt cccagagggt caggtgatgg
     3661 cggatctccg cctggaaata gtgggcgggc accagaccgt agcggagcag gaactcgtgg
     3721 agatcccttc cccggctcag ttcgtacaga aacgccccca ggcacaaggc tatgagaacg
     3781 tacgtaacga cgggggttcc ccgggagggg ttgacgtcgc gcagcgggat cataccatgg
     3841 gtactcccgg tacagggttg aaagggttac tttaccagca tcaccgggca gtgggcgagg
     3901 atccccacct tgtagctgat ggtgccccag cccaggcccg gattgtctat ctccacctta
     3961 tgggagctga ggatgatcag gtcgatgtcc cggtccccgg caaaccggat gatctctttc
     4021 acccgctttc catagatgat ttccttgtcg atccggattt ccttagtctc atgaagcttg
     4081 aggaactggt ccattttctt gtgggctctt ctcccgagct tcctgtaaaa atcgtccaat
     4141 tccccctcaa acgcgtcttc aatggtctcg atcacatgga gcaaggtgag tctggcctga
     4201 tcgtggacgg cgatcttggc ggcaatttcc aaggcacgca agcttttatc cgtgagatcc
     4261 gtgggaatca ggatatgttt gaacatgcgg tgaacctccc cttccagcac ctcaaaaaaa
     4321 cgcaatcctc tcttcttcct gacccggccc ttgtcccccc ccgagttggg cgatctcagg
     4381 gggccggaat ccgacccggg ccgcgacccc ggtcccgctc ccccgagaag cggatgaaac
     4441 cgatgcgggg gacccgggct tattcccctc gggaggtgtt cgcctccttt ccccccgggg
     4501 aatgagaacg ctcccccccg gatcccgcaa cccatgagaa ccgcggtcgg ggctttcgag
     4561 gccgggacct cgccccggaa ccgtatgtcc cggagggggt atcgggcccc ggccgactca
     4621 accggaatgg tcgccctccc aaccccgggc gaactcgtta tggtagaccc gggaagcatt
     4681 gtagcgatgg gcccggggaa tcttctcggc cgggtatccg atggagacga tacaaaaagg
     4741 cactaggttc cggggcagtc cgaacatttt ctgaaatccg ctcacccgat cttcccgggg
     4801 gtagacaccc aaccagaccg ccccgaggtt ctgggcgtga gccgccagga gcaggttctg
     4861 ggccgccgcc gaacagtcct ggacccagaa ccccttgtgc ttttccagct gcaggtcccc
     4921 gcaaatgagg atggccaggg gcgcctcccg cagcatctgg gaataaggat gtaccgacgg
     4981 catggcatcc aggatcttgc gatcatcgat gaccaaaaaa tgccatggct gctggtttcc
     5041 ggcagacggg gcgctcatgg ccccctccag caatttgcgg atcgcctcac ggggaaccgg
     5101 gcggggcagg taactgcgca cacttcttcg gctgaggatg gtttccatcg cttccatgca
     5161 gggtctcctt ggtcgttcga aatcgttgaa tcggtctcgt tggaacggat tcagcttttc
     5221 acattaatca tatccgacaa gaaaataaat cctaacctca ccgatcccgg ggtcggaaac
     5281 ctcacccagg gcgcgagggg gggagggccg gatcccgtcc ctccgccttg cacgacccca
     5341 tggatggtgc tataaatcaa tccctgcccg acccgctccc gggatgcaga acccggcaaa
     5401 ccactccccg tccccccgcg atgagaagcc gtgttcgcgc agggcggggt ctcgaagggt
     5461 gggcgccgcg gtgcggttgt cgggccgtag aaaggaccca ggacatggaa ttcgagccgg
     5521 ttatcgggtt ggaaatccac gctcagctgc tctctaaaag caagatcttc tgcgggtgct
     5581 cgacgacctt cggagcgggc cccaacaccc acacctgccc cgtgtgtctg ggaatgccgg
     5641 gagtcctgcc ggttttgaac cgggaggtcg tggaatatgc gatgagaatg gccctggcga
     5701 cccattgccg catcgctccc gtcagtcggt ttgcacggaa gaattacttc tacccggacc
     5761 ttcccaaggg ataccagatc tcccagtatg agctgcccct ggcccgggac ggctgggtcc
     5821 ggatcgaaac cgagaacggc cctcgcaagg ttcgaattca tcgaatccat ctcgaggaag
     5881 atgcgggaaa gctggtgcac gacgaacacc agcccttcag ttacgtggat ttcaatcgga
     5941 cgggagtccc cttgatcgag atcgtgagcg aacccgatct gcgttctccc gacgaggcgg
     6001 cctcctatct caagaccctg cgcgacatcc ttcgttatct cgagatctgc gacggcaaca
     6061 tggaagaagg gagcctccgc tgcgatgcca acgtttccct gcggccgtcg ggcaccacgc
     6121 ctctgggggt caagaccgag ctcaagaaca tgaattcctt tcgaaacgtc cagagggccc
     6181 tcgagtttga aatccgcagg caaagggccc ttttggaacg agggcaatcc atcgtccagg
     6241 aaaccctcct ctgggacgca aaccggaacg taacggtaag catgcgcgga aaggaagagg
     6301 cccacgatta tcgttacttt cccgatcccg acctggtgcc cgtggagatc gactcgggct
     6361 ggatcgaaga agtgagggct caacttccgg agcttcccga tgcaaaaaga gagcgcttca
     6421 tgaagcagta tgctcttccc gaatacgacg cgcaggtgct cacctctcaa aaggccttgg
     6481 cccggtactt cgaacaggtg atcgagactt ttccccagcc gaaggcggtg agcaactgga
     6541 tcatgtcgga gctcctgcgg gagctgaaaa aggaagagcg ggaaatcgaa acattcccgg
     6601 tttcccccga tcgcctgggc gaactgctcg ggctgatcgc ctcgggcgtg gtgagcggca
     6661 agatcgccaa gacggttttc gaacagatgc acacgacggg caagagtgcc cggcaggtcg
     6721 tggatgagca gggccttgtc caggtgacgg accaggacgc catcgaggca ctggtggacg
     6781 aggtcctctc ggagaacccc cgggaagtgg aacagtaccg gggcgggaaa gagaagctta
     6841 tggccttctt tgtcggccag gtgatgagaa agagccgggg caaggccaat ccccaggcag
     6901 taaacgaaat tttgcgaaag aaactggccc gtaaggaatc cccctgatcc ccccacggac
     6961 ccccgggaac cgtcggcggc actccctttt gcccggagca cccgcggggg aaccaaggcc
     7021 tgtccgtttc tccccttgat gacggttagg agccgagtat gccggaaaaa tggatgcctc
     7081 ccatctactg ggaaggagac gtggtcgtaa ttctggatca gcgccttttg cctcaccgcg
     7141 aggtaaccct tcggtgcacc acggctcggc aagtgatcca ggccattcgg aacatggcca
     7201 tccggggggc ccctgccgtc gggattgccg gtgccatggc cctggcccta ggagccaaat
     7261 ccattcggga agaagatccc cgggcctttg tgcgcaaatt ctttcacctg tgccggcagg
     7321 tgaaggcggc ccgtcccacg ggcaacaacc tggggtgggc cgtggaaaag atctactcca
     7381 tcgtggtgga aaacccccag gccggggtgg aggacatgcg gcggctcatt cgaaagaaat
     7441 ctcaggagat cctcgaagag gacgtgtcct ccaatctcgc catgggggcc tggggaaagc
     7501 aggtcatgcc ccagggagcc cgcatactca cctactgcaa cgccggggct ttggccacag
     7561 gggactacgg aacggccctg ggagtcattc gcgccgcctt cgccgcagac cccgacattc
     7621 aggtgttttc gtgcgaaacc cggcccttcc tccagggcag tcgcctcacc gcctacgaac
     7681 tcaaacaggc ggggatcccg gtaaccctca tcacggactc cgcggtgggc accctgatgc
     7741 aacagaatcg catcgacgtg gtggtggtcg gcgccgaccg catcgccgcc aacggggaca
     7801 cggccaacaa gatcgggacc tacaccgtcg ccgtcctggc ccacgcccac aacgtgccct
     7861 tctacgtggc ggcgccccgt tccaccatcg acccgaccct gagccacggg tctcagattc
     7921 ccatcgaaca gcgggaccca aaggaagtga cccacttctt gggacgccgg gtggccccgg
     7981 acggggtccc ggcactcaat cccgcctttg atgtgacccc gcatcgatac atctccggga
     8041 tcatcacgga ggtggggatc ctgcgcaaac cgttcgggaa agccattcga aaggccctgg
     8101 aaacctgagc cccccgcggc ggggaattcc gtttggggag atcatctccc gggggagttg
     8161 aaaagggcgt ccatgacttc ctggagggat cgaaccccga ggcagttcgc cccgagttcc
     8221 cgggagaggc gctccacgtt ccctgccggc accaggtgct tccggaagcc caaggcatgg
     8281 gcctcggcca ggcgcgggac gctgtggccc accccccgca cttcccccgc cagccccacc
     8341 tctccccaca ccacaaggtc cggaggcacg ggacggtcca ggtaggagct gatcagggcg
     8401 gtcacgacgg caaggtccgc cgcgggctcg atgatcctga tgcctcccgc cacgttcatg
     8461 aacacgtcct gctgggcgaa gctcagcccc accttctttt ccaggaccgc caccaagagg
     8521 gaaagacggt tcacgtcgac tcccatggtc gttctgcggg gctgaaccca gcccgtggcg
     8581 ctcaccaacg cctggagttc caccaacaag gggcgggtcc cctcgacgga acagacgacc
     8641 acggaaccgg aaacccccac gggccgctcc gccagcagaa gccgggacgg gttggggacc
     8701 tcctccaatc cctcttcctt catctcgaac accccgattt cgttggtgga cccgtagcgg
     8761 ttcttcacgg accggagcag tcgaaaggcg tgggttcgat ccccttcgag gtagagcacc
     8821 gtgtccacca ggtgctcgag ggccttgggt ccggcgatgg cgccctcctt ggtcacatgg
     8881 ccgaccagga tcacgctgat ccccttctcc ttggcaaccc gcatgagtct cactgcgcac
     8941 tctctcacct ggctgatgga acccggggcc acatcgaggg cttccgaata cagggtctgg
     9001 atggaatcca cggccaggat ttccggggca agggtctcga tcacctcgat gaccgactcg
     9061 agggaacact cggaatacgc caggagccgc tccgaacgta cccccagccg ttccgcccgg
     9121 ttcttgagct gatgggggct ctcctccccc gaaacataaa ggacctgccg gtcccgggcc
     9181 atgcgggcca gggcttgaag caacagggtg gacttgccga tacccgggtc tcccgccaac
     9241 agcaccatgg agtcgggcat gagtcccgac cccagggtgc ggtcccactc gtgaatgccc
     9301 gtgacgattc gggcccggtc ctgggaggaa atctcgtgga agggcaccgg acgggacttt
     9361 acagaggggg ttgccggggc ccgggaggac cctttggcgc ttcgttcctc caccatgccg
     9421 tcccaggttt gacattcggg gcatcgaccc agccacttgg gactcaggta cccgcactgc
     9481 tgacaacggt agacgattct tttctccgcc atgaagaccc cgtcccggtt cctgaggatt
     9541 ctcgtgcgtt ctgtcgatca ggttggcaca taccacgtct tgcatctttc gagggctcga
     9601 ccccgtatgg ttctacggcg tgtccgacag tatagaccat ttcccccctg tttcccacgt
     9661 cgggtttttg tcggtttcgt ggtcgatccc gggtaccttt ggagggcgca acccgccgga
     9721 ttgttcgatt gacaaaggga agaagatcgg tttatatacg ccacatgtac atttctgggg
     9781 gaaagcctgg agaacgtttg gcgaagttcg aggcgaagac gcttttatca cgaggagttt
     9841 tccaaatggc aggaaacatc attgaaattt ccgacagttc cttcgaaaag gaggtattgc
     9901 agtcggatgt ccctgtgctg gtggatttct gggcgccctg gtgcggtccg tgtcgtgcca
     9961 ttgccccggt catagaagag ctgtccgggg aatacagcgg aaaactgaag gtggcgaaat
    10021 gcaacgtgga cgacaacccg aagacccctt cccgcttcgg cattcgggcc attcccactt
    10081 tgatcctttt caagggaggg agtgtgagcg agcagatcac cggggccgtt gccaagtccc
    10141 agattgcatc cgccattgat aaggttgtca gctaatctcc ttaccattct tcccactcct
    10201 gaacggaaga acgcacggcc cttctgacct tgagctttcc gagcattccc atgaacgatc
    10261 tgtttgacat agcgattatc ggaggcggcc ctgcaggact gacggcgggc ctgtatgcgg
    10321 caagggcccg tctcaaggtg attctactcg agaggatggg gttcgggggg cagcttctca
    10381 cctatgaaaa agtggacaac tacccggggt tccccgaggg catcagcgcc ttcggcctga
    10441 gcgagctcat cagcgctcag gccctccgct tcgggctgct gacccgaaac gccgaggtga
    10501 cgggcctgga gttcgaagca cccatcaagc gggtggtgct tgccgacggt ttcattgagg
    10561 ccaagacctt gattctggct tcgggagctt ctcccaacaa actgggggtg aaaggggaaa
    10621 cggaattcac gggccggggg gtctcttatt gcgccgtctg cgatgcccct ttctatcgca
    10681 atcaggaggt cgccgtggtg ggaggcggcg acacggccat cgaagaagcc gtgtacctga
    10741 cgaagttcgc cgggcgtgtg catgtgatcc accggcggga ccgcctccga gccacgcaga
    10801 ttatccagga gaaggccttc caaaacgaca agatcgattt tatcctgagc accgtcgtca
    10861 aagagattcg aggcggcagc gggggagtcg agggtttgat cctggaagag gttcccgggg
    10921 gtcggacgtc ggagctggcc gtcagcggag tctttgtctt tgtggggatc caccccagca
    10981 ccgactatgt ccctgcaagc ctggaacgag accccttggg ctttatccgg actaaccagg
    11041 aaatggccac ttcggtcccc ggcgtgtttg ccgccggaga cgtccggtcc aaggagctgc
    11101 gccagatcgt gaccgccgtg ggagacgggg ctacggccgc attcaatgcc ggtcgatacc
    11161 tcgaggcgca ttccctttaa cccatatccc ttatccaagg aatcagcatg tctccttcct
    11221 tccccatgat tcaactgcag cgcttcgcct gcgccctcat cctggcgggg gtcgtcctcg
    11281 tgaccggcgg atgcgggacc aacctggtac aattctattt cggggacctg ttcggcaaga
    11341 cctcttcgac ggtgaacagg aacgctgccc aactggccca ggaaggcatg cagaagatgc
    11401 gtcaaaagaa atacggagat gctctcaagg acttcaaaca gctcaaggag cactatcctt
    11461 acagcaagta tgccatcctg gccgaactca agatcgggga cgcccatttt caaagaaagg
    11521 aatacagcga agccgccatc gcctatgaag agttcgcccg gctccatccc cgcaacgaag
    11581 tggtgcccta cgtgctgtat cagatcggca tgtgtcattt tctgagcttt tcgacggtgg
    11641 accgcgatca agaggagacc cggcttgccg tcgaatccct ccagagggtc gtccaggcct
    11701 atcccgactc cgactacgcc cagaaggcca agaagcagct caacgaatgc aaaaagcgca
    11761 ttgtggccca cgactaccag gtcgggcggt tctactaccg gcagggcgaa taccgcgccg
    11821 ccaaggaacg ccttcagaaa gcggtcatga cccaccccga tgcggtgaaa gaactgggat
    11881 acctggattc cgtcaacaag atgctggccg tctgtgaaaa ggagatcagt aagggagaaa
    11941 agaagccgag tatttggacg aggttgggat tctagccgtt gccttcccca ccttcattcc
    12001 gttccccttt cgcggacaga aggggaacgg accacaccgg gtacatgtgg agctcgaagg
    12061 agacaggaaa aatctgagct cgaatgcctt tttggtctag aaagatttgc cgaaatgctg
    12121 taatgctcac cacgtctttt ccagtgccgt gggggagcgg attgatcggc acgtcctaaa
    12181 aaaagccttg tccatagatt ccggtgatgt ccttacattc cctcggagga gcgctgttcc
    12241 cctaaaaccg cgttgaatcc ctgcgatacc cccggccccg acagagcagg ccgcaagtcc
    12301 aaaccgagaa aggatatccg gtaactatcc aagcatggag acaaaagaac atgcccgaag
    12361 caacatcctc agcaagtgat ccctccaaga gggaagccaa agagcaggaa tccagggagt
    12421 ctcaaagaaa cgagatgaac ctggtggagc tcaaaggcag gaacattcgc gaactactgg
    12481 gcacggccaa ggccttgaac atcgagggag ccagctccat gcgcaagcag gagctgatct
    12541 ttgcgatcct gcaggcccag gccgaaatga gcggtctcat ttacggcgag ggagtcctgg
    12601 agattctgcc cgacgggttc ggtttcctgc gaagccagaa ctacaactac ttgccgggac
    12661 ccgacgacat ttacgtgtcg ccctcccaga tccgtcgttt cagccttcgc acgggagaca
    12721 gcatttccgg ccagatccgt cctcccaagg acaacgaacg ctacttcgcc ctgctgaagg
    12781 tggaggcggt aaactacgag gatcccgacg tcgcccgcga caagatcctc ttcgacaacc
    12841 tgacccccct gtatccggac cgtcggattc gtctcgaaac ggtggcggat aactactcca
    12901 cccgggtcat ggacctgctc acgcccatcg ggctgggaca acgcgggctc atcgtcgccc
    12961 agccccgtac cggcaagacc atgcttctcc agaacatcgc caacgccatc agcatcaatc
    13021 acaaggacgg ctacctcatc gtgcttctca tcgatgaacg ccccgaagaa gtcacggaca
    13081 tgcagcgcaa cgttcgggcg gaagtggtga gctcgacgtt tgacgaaccg cctcagcgcc
    13141 acgtccaggt ggccgagatg gtgatcgaga aggccaagcg gctggtggag cacaaacggg
    13201 acgtgatcat cctcctggac tccatcacgc gactggcccg ggcctacaac tcggtggtgc
    13261 cccccagcgg caagatcctg tccggcggac tcgattccaa cgcccttcac cggcccaaga
    13321 ggtttttcgg gtcggcccgc aatgtggaac agggaggcag tctgaccatc atcgccacgg
    13381 cgctgatcga cacgggcagc cgcatggacg aggtgatttt cgaggagttc aaaggaacgg
    13441 gaaacctgga gatcgtcctg gatcgaaagc ttgcggaccg aagagtgttt ccggccatcg
    13501 acatcaacaa gtcgggcact cggaaggagg aactgctcat ttctcccgac gatctcaatc
    13561 gcatctggat ccttcgcaag cttttgtcgc ccctcaaccc catcgacgcc atggaatttc
    13621 tcctggacaa gatgcagggc accaagcaca atgcggattt tctggcatcc atgaaccggt
    13681 agggggaagg ggctcatgtg tccccgcgcc ctccggaacc cggaacggct tccgggtctg
    13741 cataggcctc ccctccgggg ggccttgggg tccggaaagc gcaggggggc ggacggaaga
    13801 aagcgtttgc cctcgactcg agaaggaaat tgcatttgcc tgggaagcac tcagggttta
    13861 gaatatcttg tttgactgag tccgggggat acgaaggagg aaatgaaagg ccatgaaaga
    13921 caacatacac ccgcaatatc atgaaaccgt catccgttgt gcctgtggaa acgaaatccc
    13981 taccgggtcc accaagaagg agatccgggt ggaaatctgt tccaagtgcc atccgttcta
    14041 cacgggaaag cagaaactgg tggattcggc cggtcgcatc gaaaggttcc tgaagaagta
    14101 cgaaaagtac caaaccgact catcctccgg gtccaagggc tagggtttcc tcgtccggca
    14161 agattttcgc cggccgcgga ccttccgacc cggtccattc cgggttgccg gggaacaaga
    14221 acgggtagtc gagcgccgac tatccgattg ttttttgggc tcgccgattc ccccggcgat
    14281 ggtttcccaa gggcaaacgg cacagggtcc ggaacaagac cggccaaggc ccccagcata
    14341 taggcccaat cgccatgttt gatcgactcc aaagcgtgga agaaaagtac cgcaagctcg
    14401 aggaagacct ctccaacccc gagatgctgg ccaatcagaa agaatatcaa cgggtcgcca
    14461 aggaacgctc ggacgtggcc cccctggtcg aggtgtatcg acagtacaag gccctcaaac
    14521 aggaattgca ggacaaccat gagctcctca atgaggaacc ggaccccgag atgcgggagt
    14581 tgattcgcca ggaaatcagc gacctccggc agaagctcca ggctgtcgag gacgagctca
    14641 agttcatgct ggcccccaag gatcccaacg acgaaaagaa cgtcatcctg gaaatccggg
    14701 ccgggacggg gggcgaggaa gcggcgctct ttgccgccga tctgttccga atgtacaccc
    14761 gctacgccga aatccgcagg tggaaggtcg aggtcctgga cagccatccc acggggatcg
    14821 gcgggttcaa ggaaatcatc gcggccatta acggcaaagg ggcgtacagc cgattgaagt
    14881 acgagagcgg ggtccatcgg gttcagcggg ttcccctcac cgaaagccag ggacgagtcc
    14941 acacgtcggc ggtcaccgtc gcggtgctgc ctgaggccga agaagtggat gtctacattg
    15001 atcccaacga cctgcgcgtc gacgtcttcc gttcctcggg tcccgggggc caaagcgtca
    15061 acaccaccga ttcggccgtg cgcatcaccc acttgccctc gggtcttgtc gtgatctgcc
    15121 aggacgagaa atcccagcac aagaacaagg ccaaggccct caaggttctg cgggcccggc
    15181 tcctggacca gatgcaaagg gaacaggaag ccaagatcgc ccaggaacga aagagccagg
    15241 tgggaaccgg ggaccggagc gagagaatcc gcacctacaa cttcccccag aaccgtgttt
    15301 ccgaccatcg cgtcaacctg accctctacc gattggacgc tgtgctgaac ggcgaactcg
    15361 atgaaatcat cgatcccctg gccgctcata gccacgccga agcgcttcaa cgaatggatt
    15421 cctgaggaga aagccgggag ctccgcaaga ttcttccggg cgcggtgctt cccgggcagg
    15481 tctctccccg gaacccccac caatccccgc aggattgaag ggatggaaga gagctggacc
    15541 atcctgaaag tgctccagtg gaccaccggc tatttcggcc ggaaagggat cgagcagccc
    15601 cgggccaatg ccgaggttct gctggccttc gttctgggca tggaacgtat cgatctttac
    15661 ctgcgccacg accaacccct caccgcttcc gagctctccc gctaccgaga ggtggtgcgc
    15721 cgccgggcct tacgggaacc cacccagtac atcacccggc gacaggaatt ctggtccctc
    15781 gagttcgagg tcgacccggc ggttctcatc ccccggccgg aaacggagct gctggtggaa
    15841 aaagccctcg atctcgtggg ctccgatccc aagaaagtcc tcgagatcgg cacgggatcg
    15901 ggagccatct ccgtggccct ggctcatgaa tgcggatccg tccaaatcct cgccacggac
    15961 aagtcgccgg aagccctccg ggtggcacga cgcaacgctc gaaggcacgg cgtcctggag
    16021 cggatttcct ttgttgcagc ggaccttttc gaaggcctgc atcccggcaa cgcgcggttt
    16081 catctcgtag tcggcaatcc gccctacgtc agcgacgagg aatacgcgga tcttcccgaa
    16141 gaaatctccg ggcacgaacc cgccatggcc ctcctgggaa aaggcccccg ggggctggac
    16201 gtcgtgaggc gcatccttca aacggccggg gcgtttctca cggctcccgg ggacctcctg
    16261 ctggagatcg gccggggcca ggcggaaatc ctcgaacaag agccgaagct cttcccgggt
    16321 cttcgactcg agggatttct gcgggatcat tcgggcatcc tgagaatcat gcatcttcga
    16381 aaggtcatcg ggtgacacat ggacaaactg gtcattgaag gcggactgcc gctcgaggga
    16441 gaagttcccg tcagcggagc caagaatgcg gcgctggcat tgatggccgc caccctgatc
    16501 gccgggggca aacaccggct cgatcacgtc ccccgcctga gggacatccg taccatggaa
    16561 ctgctcctgg agcacatggg ggccgtttgc caccacaacg accacctagt gatcgacacc
    16621 ggcaacattc actgcctgga agccccctac gacctggtca agaccatgcg tgcctcggtg
    16681 atggtcctgg ggcccatggt ggcgcggttt cgccgagccc gggtgtctct gcccggcgga
    16741 tgtgccatcg gagcccgccc catcaatcta cacctcaaag gcctggaaca actgggggtc
    16801 gggatcgagc tcgatcacgg ctacgtggtt gcgaaagccg accggcttcg aggcgccgtc
    16861 atccccttcg accaggtgac ggtgacgggt acggagaacc tgatgatggc ggcgaccctg
    16921 gccgaggggg tgacggttct caggaacgcc gcccgggaac ccgaagtggt ggccctcgcc
    16981 gaatacctgc gcagaatggg ggcccagatc cggggagagg ggacctccga gatcgtcatc
    17041 gaaggagtga aagagctggt ccccgcttct tttcgggtca ttcccgatcg aatcgaggcc
    17101 gggacctacc tggtggcggc agggattacc ggcggcaatc tcaaagtgac cggctgccgc
    17161 ccggaacatc tcgatgccgt catgggcaaa ctcgagctgg ccgggatgac cttccaggtt
    17221 tcgggagact cgatccaggc aaccggcacc ggacaaatcc ggagcatcaa cctcaagacc
    17281 tggccctttc cggggttccc cacggacatg caggcccaat tcatggcgct gatgtgcctg
    17341 ggaaacgggg tgagcctcat taccgagcag atttttgaaa accgcttcat gcacgttctc
    17401 gagctgaagc gcctgggagc cgacatcgaa ctcgatggcc gcagcgccgt ggtgaaaggg
    17461 gtccgtgggt tgtccggggc ccccgtcatg gccacggatc tgcgggcttc cgcttccctg
    17521 gtgctggccg ggctggctgc cgaaggcatc acggaagtga accgcatcta ccatctcgat
    17581 cggggctatg aagccattga aaagaagctc tccgccgtcg gggcccggat ctggcgggaa
    17641 agaggcgttt gagacccgac tttctttcaa tcgggaaggg gtcgtgccgg agccaccggc
    17701 cttcggtccc gcgggcgagg gcgctcccgg gcccagaccc tggcctcgac ccctgttgct
    17761 cctggctgcg gctttggctg cgggaatcgc cctggagtcc caagtcccgg agatgtttcc
    17821 ggaaccctgg gtctgggcgg ccccggccct acttttcctc ctttccctcc tgggtttttt
    17881 tcccgcttgc cgaaggttgc gcagccctcc taccgcagtc atcctctttt tcgccctcgg
    17941 tgcggctctc agccgacagg ccgacccccc gctcccttct cccccctctc tccaatcctt
    18001 cctgaaacag ggacacaccc ttttcctggc cgaaatcgat cgtcctcccg aatttcctcc
    18061 caaccggacc cggcttcgtc tccggttgca caaggccatc cctgacgggg gatcggtccc
    18121 cgtggaaggg aaagttctcc tgaccatcgg gcaggcccgg caaacccccg ggcggtacct
    18181 tttgggagac atcctgctgg ttcgcctgaa cctcaggccc cttcataatt tcaacaatcc
    18241 cggcgggttc gactacgttc gagccatggc ggagcagggc gtttttgccc gggcctatct
    18301 cgccgacgac cgcctggtcc tcccggtgac cggggagccg ctttccattc gacgccttcc
    18361 cgccctgatg ggtcggtccc tggatcggtt tcgacaaaac gccctgtttt ggctgcgagc
    18421 ccacctggac cgggatgagg cggatttcta tgcagccctt ctcctgggtt accgaatccc
    18481 ggacgaatgg ggcgatcatc tcagtcgaac cgggctcact caccttctct ccatatccgg
    18541 tctccacctc gggatggtct ttttggcgag cttctggctg gttcgcagac tcgttcgccg
    18601 gaccctcccc aatgtgctga gaaagagaga cgaccagtcc atcgccctgt ggttttccct
    18661 gggggcggcc gccgtgtatg ccctcatcag cggcatggcc ctccccacgt ggcgctccct
    18721 gttcatgctg attctctttt tccttgccgc ctcgaggtat cgccgtgcgg attccctcac
    18781 cggcctggcc ctggccgccc tcctcatcct ggcactttcc cccaaggccc tcgggcagat
    18841 ctcctttcaa ctctcctttg cggccctgtt cgggttgttt tgcatctacc cccacctcga
    18901 ccgcctgcgt caattcctgg gccgccgggc cgcctttccc gaaatgcccg ggaggaccct
    18961 catcaaactc atctgcgacg ctttctgggt ttccctggcg gccaacgtga tggtccttcc
    19021 catagtggcg tatcatttcc acgggatctc cccggcagga ttcctagcca acgctttgct
    19081 ggtcccctgg acgggattcg tcgtccttcc cctggggctc ttgagtctgg ccgttttcgc
    19141 tctctggccg cccctgggac ttccgttcct cttgctcggc ggctggtgcc tgggaatcac
    19201 cctgcctgca atccgctggt tcagttccct gtcatgggcc ttcttctggg tgggggccgt
    19261 ccccctctgg ggattggcgg gctactacgc cgccgtggcc gttctccttt gcccgtggag
    19321 cggcaagacc cgggtggcgg cgctggccgc tttgctcctg tgggtcgtgg gaatccaaca
    19381 ggcatttcgg ctttccgcac accccaattc ctcctccctg cttcgggtca ctttcctgga
    19441 cgtgggccag gggagctcca ccctcctcca gtttcccgac ggccgcgtga tgatcgtgga
    19501 cggcggaggt ttctgggaca attccttcga tgtgggacga ttcgtcgtgg cacccgccct
    19561 ctggcacctg ggcatccgga aaatcgactg gatggttttg tcccacgacc accccgatca
    19621 ccgcaacggc cttcggtttc tgctgtctca tttttccgtg ggggaattct gggagagcgg
    19681 gattcacgac aagggggcgg agctcgagcc cctgagcacc gtcgcctttc ggcgcgcaat
    19741 tcgagtccgc tccttcacgg aactggcggg agcccaccac ttcggtccgt gccgggtccg
    19801 gctgcttcac ccttccccgc actacctccg ggaagagtgg gaccgaaaag atttgaacaa
    19861 cctgtcggcg gtccttcgag tggatttcgg cgggactcac ctggtactgc cgggggacat
    19921 cgatcgttcc atcgaggagc ggatcctggg tggggaggac ctcccggggc aggtcctcct
    19981 tgcagccccc caccacggga gcgagaggtc cggcagcccc ctgttgttcg atcgactccg
    20041 cccccgggcc gtggtgttct cctgcggagc cgacaactgg tttggatttc cctcttccgc
    20101 cgtgctacaa gaatgcaccc gccgcaacat ccggatcttt cgaacggacc tccagggggc
    20161 cgtccgggcc cgttccgacg gggttcgatg gaccctgacc ccccacttgg aggggaaaca
    20221 gcccggggga tcatgaccct tcccttctac cgtgacgaaa ccaatggaga gagagaaaaa
    20281 tgcgaacgta ttttttcggg gatttgcacg gcaacgcgga ggccctcgag gcctgcctgc
    20341 gccacatgga ccgggtccgg cccgatgccg ccctctgcct gggagacctg gtggggtggc
    20401 tgcccttcgg ggatcgtacg ttgactcgaa tgcgggaact cgacctcccc acggtggcgg
    20461 gcaatcacga cctgctcgtg gcgggcgccc tgacggactt tccggaacag atcgaccgga
    20521 tgcaggcatc cgcctacaac gccgggctcc tctccacggt gcccggagcc gtcgagtacc
    20581 tgctttctct gcccttatcc ctggaggaga aggattttgc ggtggtgcac cacagcccgt
    20641 ttcacctgcc ttctccgggc agggtcccca ccattcacag ctttcactac ctggattccc
    20701 gggccttggg cgagtccctg gatttgtggc gggggtattc cagacgcctc atcttcagcg
    20761 ggcacgacca cgatcccgcc gtgttcgagc tttccgacgg ggccgaacca cggttggaaa
    20821 acgtgaaggt ctattctccg ccgccttcgg ggaacctcac gatctccttg aaaagcggcg
    20881 caaggtactg ggtgaaggga ggcagcgtcg gaggacccta tcgggacggg attcccgcag
    20941 cgaattccgt cctctacgac tcggaggcgg aaaccgtcac cctgtttcgg gtcccctatc
    21001 ccacccggcg gctcatggag gagctgacct cccatccctt ccaccggaac ctgccgacga
    21061 tgaagaagta catcgagctt ctgtcctcga cggctacgga tttgtcaggg cccgaaccac
    21121 ctcgggaacc gtcttgaagg tatggcggaa ccaggtcctg agaaaggcgt catcggcgac
    21181 cagccgggga gggtctcccg gtcgaatggg cttttcgtcc acattgaccc gggtgcctaa
    21241 cgcgttttcc accgtggaaa cgagttccct cacggaggtg ccggacccgc tcccgacgtt
    21301 ggaaatgagc cgttcccggg agcgaatgac gtccagggcc ttgatatgcg cctcggccag
    21361 gtccatgacg tagacgtagt cccggatggc cgtgccgtcg ggggtggggt ggttcgttcc
    21421 gaagagctgg aagggggcgc cggttcgagc ggccttcatg aggttgggga gcacatgggt
    21481 ttcgggttcg tgcttctcgt agatttctcc gtcgggatcg ttgccgatga cgttgaaata
    21541 ccgcaaagcg gcaaaccgga ttcccaccac cggcgccacg gcccggatca cctgttcgca
    21601 caggagcttg gaggtaccgt aagggttggt gggttcctgg ggatgatctt cctggatggt
    21661 gggtgtgacg gcgttgccgt aggtggcgca ggaactggag aagaccaggt acttggttcc
    21721 ggctcgtttc atggccttga gcagggctac ggtgccgccc acattgttgt cgaagtactc
    21781 ctcgggaatc cgggtggatt cttccacgta agctttggcg gcgaaatgga ttaccgcgtc
    21841 cgcttgaaat cccgagagcg cccgaaagag agcctcttcg ctgcgaatgt ccccgaaagc
    21901 gaaggggccg aatttcaccc attcgggcca tccgttggac agattgtcaa aagcgatggg
    21961 ggtatggccc acctgcttca atcgcttggc tgtatgggac ccgatatatc cggccccacc
    22021 ggtgactaga atcttcacgg atgcctcctg atcctggggg tgcgacggcc tgggggtccg
    22081 cccgacgggg accttttttt ccttcccctc tttcacggcg gattgggagg ccatatacca
    22141 cttttcccgg ggtttggaca ggatttgtcc gccccgagga cacccgggag ccgcacggcc
    22201 tcgcttcccg ggtccctgtg ccgggagacc gtccccgccg acgaaggttt ccggcagctc
    22261 atctccccgg gctgagcacg cccggtttgg ttcttgtggg ggcgggcggg acgtcccgtg
    22321 cgaaaaaggg cggggaaacc gtccgttttt gtttgcaccc ggaccggaaa cccgatagcc
    22381 tgaaaccgtc tgcatcatca gctacgaaag acaggacgcg tgttgacggc cgaagcccgg
    22441 tgtgcacctc gtccccatcg gaaaagaagg ggctttcccc ggctcggaga gcttccggaa
    22501 aacggagcgt cgcccgagcc ggacggcggt tttcatccac ggcctgtgcg gggttaccac
    22561 ggacgagccg gcaaagcgct gggaatgagc cggatgacgt tccatgcccg gtttccgagc
    22621 aagaccaccc tcgttgaagc cgcactcctc gcccagccca atgaaatcga agagcagctc
    22681 gaagagacct gtctcgagaa aggtcccggg ggcctgcagc cggtctccgg caaagcctcg
    22741 ggggccgtga ttgcccatgg gcactcattc cgagacgcct cggtggaacg cacgcgaaag
    22801 gctcgaggcg gcggggttgt ccggacaagc ctttgtacgg tcgccttccc ggcggtttct
    22861 gcgggaaggg tgcagggtcg agatgctccg agggggagcc gtcggcccaa gcaaggttcg
    22921 gaactctctt ggagggtgca ggccttgacg accccccaag gagtcggcga ggtctcacgc
    22981 ccgagttcgg ctgcacggcc gtcttcagcg gattttataa aggtgctgac cacgaacagg
    23041 aggggatcga gaggatgagc ggcacgaccc atcgttggtg caaagagctt cttaccgtct
    23101 gccgggtgtt gccgctactg gtcctggccg cgagttgcag tccttcggca tccccggaca
    23161 aggtccaagg gtacgtcgaa ggggagttcg tctacgtgtc ttctcctctt tcgggaacac
    23221 tcaaggagct gcaggtgcaa cggggggatc gggtcggagc gggggatccc ctcttcagcc
    23281 tggacgacac cccggagaag gcggccctgg aggaggccct ggggcgcctg gcccaggccc
    23341 gggcgaatct cgaggatgcc cggaagggga agcgtccttc ggaaatcgag tccctggagg
    23401 cgcagatcgg acaggctcgg gcggccctgg ccctctcgga aaaagaattg gcgcggcagg
    23461 agaagctcat gcgcatcccg ggggcaacca ccgaacagga cctggaccgg gcacgggccg
    23521 cccgggacca ggatgcccgg cgcacggcgc agctcgaggc cgacctgaaa acggcccggc
    23581 tgggtacgcg gacggacctg gttctggccg cgcaggcggc ccaacaggcc caggaagcgg
    23641 tgctggccag ggcgcaatgg gacctctccc agaaggctca gagcgccccc gattcgggat
    23701 tggttttcga caccctttac cgggtggggg aatgggtggc tgcgggccgc cccatcgtat
    23761 ccctgcttcc ccctcaaaac atcaaagtgc gcgcttttgt tccggagacg aagatcggcg
    23821 ccgttcgtct cggggacccc gtccgggtcg gggtggacgg gatcaaggag cccctcatcg
    23881 gcagggtgag cttcatttca cccagggcgg aattcacgcc cccggtgatc tacagcctcg
    23941 agtcccgcgg caagcttgtt ttcatggtgg aggcggtctt tgacccccag accgcggtga
    24001 agctgcatcc cggtcaaccc gtggatgtcc ggttcggatc ttgagccatg acggatgaac
    24061 tctccattga tgtacatgga atgaccaagc gtttcggcag ccttacggcc gtgaaccgca
    24121 tcgacctcga ggttcggaag ggcgaaatct gcggctttct gggacccaac ggcagcggca
    24181 agaccacgtt catccgaatg ctttgcgggc tgctccgccc ggatgaaggg agcggcacct
    24241 gcctgggcct tgatgttatc cggcaaagcg aggccatcaa gcgccaggtc ggctatatga
    24301 cccagcgctt cagtttctac gaggatttga gcatcgagga gaacctcgat ttcgtggccc
    24361 ggatgtattc catgaacgac cggcgccggg ccgtgaacga aagcctcgag cggctggggc
    24421 tgagcacacg ccgcaatcag ctggcgggaa agctctccgg gggctggaag cagcgtcttg
    24481 cccttgccgc ctgcatgatt caccatccga agctcctctt gctcgacgaa cccactgccg
    24541 gggtcgatcc caaggctcgc cgggaattct gggacgaaat tcaccgtctc gccggagagg
    24601 ggctcacctt tctcatcgcc acccactaca tggacgaagc cgagcgatgt catcgcctgg
    24661 ccttcattct ctacgggaac ctgctggtcc acggcacggc ttccgaggtg atcgccaagt
    24721 cccgcctgac ctcctggaca gtgaccggcc ccgacctggc ccggctctcg gaacggctcc
    24781 ggcaactgcc gggagtggaa caggtggcgg ccttcggcaa cacccttcac gtgagcggag
    24841 gggacgccga ccggctcgaa gaggccgttg cccctttcag aatcccgccc tatcaatggc
    24901 agaggactac gtccgggctc gaggacgtgt tcattcacct catggatgga tccaacgggg
    24961 gccgttcagc atgagaaaac gccgcgggtt ttccttggga cggttttggg gaattgtcgt
    25021 caaagagttc atccagatgc gccgggaccg gttaaccttt gggatgatga tcggaattcc
    25081 gctcctacag ctgatccttt tcagctttgc catcaattcg gaccccaaga acctgccgac
    25141 ggcccttctt caggccgacc acggccccca ggctcgaacc ctccttcatg ccatcaggaa
    25201 cagcgcctat ttcaatttcg tcatggagct gcaatcggaa gaggaggcga gggatgtcct
    25261 ggcccaaggc cgggtgcagt tcgtggtcca cattccggag aacttcagcc gagacttttt
    25321 gcggggagac cgccccgccg tcctggtgga agcggacgca acggatccgg cggctacggg
    25381 caacgccgtc aattccctcg caaacgcctt gaactccggc ctcaagaacg atctcaaggg
    25441 gccgctggcc tatctggccc catgcgacag cccggtggac ctgcggattc acccccgcta
    25501 caacccggaa gccatcaccc agtacaatat cgtgcccggc ctcctggggg taatcctcac
    25561 catgaccatg gtgatgatca ccgggctcgc catcacgcga gagcgggaac ggggcaccat
    25621 ggagaacctg ctctccatgc cggcccgtcc gttcgaggtg ctgctgggga agatccttcc
    25681 gtacattctg gtgggctaca tccaggtcgc cttgattctc ctcgtcgccc actatgtttt
    25741 ccggatcccc atggtgggca gcgtcccgtt gctgctcctg gtatccatcg tgttcatcgc
    25801 cgccaacctg gccatgggga tcaccttttc caccgtggcg gaaaaccagc ttcaggcggt
    25861 gcaaatggct ttctttttct ttttgccttc cctgctgctc tccggattca tgtttccatt
    25921 ccggggcatg ccggtatggg cgcagcacat cggcgaagtg tttcccctga cgcacttcct
    25981 gcggatcgtc cggggcatct tgctcaaggg caacgacttc aacgacatcg ccgtcgatct
    26041 ctggcaaatc gtgctgttcg ccgtcgtcgc cctggtgatc ggggtaaaac gatatcggca
    26101 gacgctggat tgagcggcgg agtgcccccg gggccttcat acgggaagga aggctctttt
    26161 gaatcgcagc ggggtctcga gggatcacgg cagggggacc ccgagggagg aaaaccacaa
    26221 aaatgccgag gctcaagccc atccaggcac tgggacacat cacccggttg gccgttgccc
    26281 tcctgatcct gaccacctgc gcggtgggcc cggattaccg gaagcccccg gtggaagaca
    26341 tcaccccggc ggactggcga tggaagattg ccgagccgcg ggacaccatc tcccggggag
    26401 aatggtggac ggtgtttggc gaccccgttt tggccgccct cgaggcttcc gccgtggccg
    26461 ccaaccagga ccttcgggct gccgtcgccc gggttgacga ggcccggtcc gcggcaagga
    26521 tcgaccgaag cgagttcttt cccgaaatct cgctggatcc gtcggtcaag agagaacgaa
    26581 cttccggcca catgccgacc ccgattccca ttcccattcc cgcatctcac gtcaatacct
    26641 acagcgtccc cttcgacctc agctacgaag tggacgtctg gggacgggtc cggcgttcct
    26701 tcgaagccgc tcaggcccag gccgaggcga gcgtttccga ctactggagc gtgctgctca
    26761 ccctcacggc cgacgtggcg gcaaactact ttcacgttcg agccctggac tcggaaattg
    26821 ccgtgctgca acagaccatc caatcccggg aggaaatggt ccgtgtcctg gaagggcggt
    26881 tcctggcggg cgccgtcccc gagatcgatg tcgcccgggc ccgaacggaa ctggccggtg
    26941 caagagcgga cctcgccgac gcgcgacgac agagagccga aaccctccat gccctggcgc
    27001 tcctttgcgg caaaccggcc ggggacttcg agcttgccga aagacccctt gccgggtccc
    27061 ctccgcaggt tcccccgggc ctgccttcct cgctcctgga gcggcggccg gatattgccc
    27121 gggcggagcg aaccctcgcg gccaagaacg ctcagatcgg cgtggccaaa gccgcctact
    27181 ttcccgtgat cagcctgacg ggccaggccg ggtacctgag cgccgaagtg aacgagcttt
    27241 tcgccgattc cacccgggtg tggtccatca gccccggtct cagccttccc ctctttacgg
    27301 gcgggcgcac ccaggcccgg gtccggcagg ccgaggcgat ttttcgggag tccgtggccg
    27361 cgtatcgtca gaccatcctc acagccttca aggaagtgga ggattccctc gcccaactcg
    27421 tcctccgggg cgaggaatcc gtcgcccaga tggaagctgt taccgccgcc caacgatccg
    27481 tcgaactggc ccgggctcgg tacgaagccg gaaacgggag ttacctggac ctcctcgatg
    27541 ccgaccggac ccgcctccag caggagcgaa agctggcgca agcgacaggc ctccgtttcg
    27601 aagccgccgt ccacctgatc aaggccctgg gagggggttg ggagggcgga acctagcccg
    27661 gcgaccagga ggggccggtg ggcctcggct gcgggagccg gccccacgct tttcggcctt
    27721 tggcagacat gtccgtggcc ggcctttccc ccgccggatg cctgcccttg gccgtccggg
    27781 gagtgtgtga gccccccgga tttccctccg cgcggaacct cccgcgagga ttcctgaaac
    27841 cgcccttttt ccgttcgcat ggaaaaccaa ggagggtacg gtgttccaga aagcggtgtt
    27901 tcccgggaaa tacatccagg gggtcggcgc catgagcgaa ctgcccgcgc tgattgcgtt
    27961 gctgggcaaa cagggactgg tcctggcctc tcccaccagc aaggataagg tccttccccg
    28021 gtgcgggatc gacccggacg ggagcaggtt ggccatcgag ccgttccggg gcgagtgttg
    28081 tgaagaggag gtaagacggg tgtccgccgt cctcgatcga agccatgcgg atgtcttggt
    28141 gggaatgggg ggaggaaaga ccatcgacac ggcgaaaatc gtcgccgacc gggcgggcat
    28201 ccccgtcatc gtcatcccaa ccatcgcttc cacggacgcc ccctgcagcg ggtgcgcggt
    28261 gctctattcc cccgacggcg ctttccattc cgtgtgctac caaaaagcga acccggcggt
    28321 ggtgctcgtg gacgtatcga tcattgcagc ggcgcctgcc cggttcctgg tggccgggat
    28381 gggggatgcc ctggccacct ggtttgaggc tcgctcctgc catcgaaccc agtcccccaa
    28441 cgagtgcggc ggcctgagca catcggtcgg gctccacatc gcccggctct gttacgaaac
    28501 cctgctgagc tacggcccgg ccgccaggct ggcggcggag aagcacgtcg tcactccggc
    28561 attggaacat gtggtcgagg cgaatatact gttgagtgga atcgggttcg aaagctccgg
    28621 attggcggcg gcccactcca ttcacaacgg tctcaccgcc ctggcggaaa cccattcgtt
    28681 ctaccacggc gaaaaagtgg cctttggcgt gctggccggc ctccaactca cagacgcatc
    28741 caaggaggaa tcggataccg tttactcctt ttgcgaggag gtcggcctgc ccaccacgct
    28801 aagggacatc ggcttggaaa acggcgaccg ggagaaattg aggacggcgg cggaaaaagc
    28861 ctgcgctccc cgggaagccg tccatcacga agcgggcacg atcactcccg aaaaggtcct
    28921 cgatgccatg ctcgcggcgg acgccctcgg gatgcgaaga ggtggccgcg gaaacgcgag
    28981 gcgacgtggc cgttttgagg ggcgcatgaa tagacgaagt gagaatgtct gaaccggtac
    29041 ggccggggga tgtcctgacc tcaaaggccc ggtgatcgaa tttgaaagga tctcgaagca
    29101 aatcggatcg cggatttgcc gccgtccgcc gcaaggcggc aaaccaaagg ggaggacccg
    29161 tcatcgagta cgggtgccgg gacatgatct cctgccgaag cttcgggccg caaggggccg
    29221 gcgggacgat cggttctccc tcccccaagg agaacgatac atgacgattg gcgggtggtt
    29281 cttgttcctc tggcttgtct actttctgct cccctttgat atcctccatg accggattcc
    29341 atacctgggg cgggcggacg atctgctcct ccttttgtac cttatgtggc tgtactggaa
    29401 gaaaaaccga accgcctcga aagaacgcgg cagcacctct tccgacgacg ggaccttctc
    29461 gggccgggaa cgagccgagc aacagggggc cgggcaccag cgagcccaag ggcacgcgcc
    29521 ggggcaggag tcccgtgcac ggccgaggga cccgtaccgc atcctgggca tcgacccttc
    29581 ggcttcgacg gaggagatca agaccgctta tcggcagcag gccgcccggt accatcccga
    29641 caaagtctcc cacctgggag aggaatttca gaagctcgcc aaggaaaagt tccaggacat
    29701 ccagtgggct tacgagcaac tcatgaaccg ccggagaaaa gcctgacgga ggagtcaagc
    29761 gacatgacgg acgatcagag tttcgtttcc atccataccc tcaccaaccg cttcgaaggg
    29821 gaccttctga tgcaggccct ggcgggagag gaaatcccca ccatccttcg atcctttgaa
    29881 gagaccccct acgacgggct cttcattccc cagcgaggat ggggacaact cctcgtgccc
    29941 gaacccctgg cttccagggc ccgtcggatc atccaacctc tcctggacca cctggaatcc
    30001 cggggaccgt actcgaaccc cgcggacatc gatccacaac tctgggagtc tcttcgccgg
    30061 gcagacccgg acctcgtctg ccgcaacgcc gaggtgggct acgatgcgga gcgcggcgtt
    30121 taccgggttc ccttcctgag ccgggagctt cactgttttc ccggggaaga aaggatcgag
    30181 ccccgggagc ctctcccgtg caacctccag gattttcagt cgtatctggt cgtccttcat
    30241 tacctcttgg aggcccggcc cgcgagtctt gtgggcaaat ggatcagtga acgggagatc
    30301 cccggcgggg agctgttctt ccggggcccc catcggtttc ccaccgggtc cctggtccgc
    30361 tgcttcgggt ctcgaaagga tcttttccgg gaggtctccc aaaggttaca gggggttccc
    30421 gtctccatgg gggatgtcgc cttccgttac tcggtcctgc cgcgcattcc cctggtgctc
    30481 gtcctgtggg aaggggacga tgaattcgag cccgccttcc acttccgttt cgatgccggg
    30541 gtgtctcggc aggtgaaatc cctggacacc ttgtgggcca tggtaaacgt ggtgagcagc
    30601 tgcctggaga gtttggcccg ggagctgacc gaatcaacgg agtgactcca tgcgcagacc
    30661 gtttcgagtc atctccctgg ctcccaccca gaccgagatc atcgccgccg tcggcgggtt
    30721 ggagcttctc gagggagtca cggaaaattg cgactttccc gaggatgtcc gctgggtccc
    30781 cgtcttcggc tcctggcatg ctccggacct caggcgggtt ctggacgctc aacccgacct
    30841 ggtctgcacc ttcggcaaac accaggagga attgagcatg gccctggaag aatccggcct
    30901 gagggtctat catagcgacc cgggggatgt gccttcgagt ttgaagacca tcggggagat
    30961 cggttcgatc ctggggtgcg accaggcggc ccgtcgcttg atcgacgccc tggaaacaag
    31021 gctggaccga gtggaacgag ccctggggcg acgttccccg gaagaacggc ctcgggtgct
    31081 tcgaatcatg aactgggacc ccctcatcac ggtgggaccc ggctctttcc agcatgacgt
    31141 catcgaacgt gcggggggtc gaaacctcat ggaagacggg cttcagccgt actttgcctg
    31201 cgacccggag cgggtcctgg accgagaccc cgaggtcatc ttcttctgcg aaccccatat
    31261 ccgggaggtc ctcgaggcag acccggcctg gaggcggtgt tcggccgtgc gcgactccaa
    31321 gatctttatt ttcgactgcg ggttgacctg ccgctccggc ccccgtatcg tggacatggt
    31381 ggaacaactc gcccgggcac tccatcccgg gccctgaccc tcgaggcccg gacaaggaac
    31441 cggcccgggc cgattccggg atccgcgggg gtgacttccg gtcaacaaca gccttggtcg
    31501 acccgatgtc cccgttcatg actgcaagga gaattgcgtg aaccatccac gattccggag
    31561 cattggacgg aggccgcggc ccttcgcgtt ctgcacggcc attgtcacca tcctgctggt
    31621 ttcctcgggg tgggcggttc ctgcccgtgc cgaggtcaag ccgatccccg aagacctgac
    31681 gaccttgacc ctcgaggact tgctgggaat cgaggtgact tcggcatcca aaaagctcca
    31741 gagggtttcg gacgcccccg ccgccatttt cgtcattacc caggacgata tccggacctc
    31801 cggggccacg agcattcccg agctgctgcg catggtcccg gggatccagg tggcccggct
    31861 ggactcgaac aaatgggccg tcacgtcccg cggattcgaa ggtcgtttcg ccaacaagct
    31921 cctggtgctt tttgacggcc gcaccgtcta cacccctcta ttctccggag ttttctggga
    31981 agaccaggat tacctgctgg aggatattga tcgaatcgag gtcatccggg gaccgggggc
    32041 aagcctttgg ggtgccaacg cggtcaatgg agtgatcaac atcatcacga agcattccaa
    32101 ggacacccag ggtgccttga tcagctcggg agccgggact tacgaacagg ggttcgtggg
    32161 ggttcgatac ggaggaaggc tcagcccaaa cctcacctac cggttgtatg ggaagtattt
    32221 caatcggggc actttcgacg acgaatgggg aatgcagggg gccgacgact ggtgggcata
    32281 ccgctcgggg ttccgcctgg actacgacaa gcccggcaaa gacgctctca ccctccaggg
    32341 ggatctctac cacctggatt cgggttcccg ctacctcgtc cccgctttca cggcgcccta
    32401 cgcagacctg ttcgaccata cctccaacca tcccgggggc aacgttcttt ttcgatggtc
    32461 ccataccttc tctccttctt cccaggtcag cctgatcacc tattatgacc gagcccaagt
    32521 caaggacgtg attctcagca acgtgacgga taccttcgac atcgatttcc agcacacctt
    32581 gaaactcgcc ccgtggaacg aactgatctg gggactgggg tatcggtatt accgggacaa
    32641 catcgacaac tcggaggtgg ttaccttcga tccgtctcac acccggaacc agctgctcag
    32701 tgctttcatc caggatgagt tcaagttctt ggacgaccac ctgcggctca ccctgggatc
    32761 gagatttgaa cacaacgatt acacgggttt cgaggctcaa ccgacggccc ggctgttgtg
    32821 ggcgcccaac ccggaccatt ccgtgtggac ggccttttcc cgggcggtcc gaaccccctc
    32881 ccaggtcg
//
LOCUS       DAOOVC010000029        32655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_909_length_32655_cov_6.496012, whole genome shotgun sequence.
ACCESSION   DAOOVC010000029 DAOOVC010000000
VERSION     DAOOVC010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 32655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32655
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_909_length_32655_cov_6.496012"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLM79_05940"
     CDS             <1..710
                     /locus_tag="PLM79_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85883.1"
                     /translation="LESMDPPPDLQDVEPLAGLVQELGRNRDRWARCSTLCRGLEPLR
                     DPPELEAVRDLHAVVSSLEELTRSQSRIHVRSSRLRSLEEPPEAWDPTPLKDLIRVLE
                     DLEIGCRRTRKRCETTERLQPVPTPGDTRDLEETVLALEKTRDHLKRTGDRLSLLQAT
                     EPCPQLHPLKDLDEWIAEESRLQNEQSRLERRHAECLKAIQRKKKEIEGVLREIGACP
                     LCGQAFDLTHFLEKDHG"
     gene            703..1665
                     /locus_tag="PLM79_05945"
     CDS             703..1665
                     /locus_tag="PLM79_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV85884.1"
                     /translation="MVETDRTPSKPYQGVLFIGDPHLAAFPPGHRLDDYPQTVLGKLA
                     YCLEAAGRHRCLPVILGDLFHVPRNNPNSLLVDLIELFRGSRPWVLVGNHDKHEARLT
                     RDVSLSVLEAAGVIRLLKESGPADSVLLGGARVLIGASPDWTPLPKEVEPRDHDRVLW
                     LTHHDLALPGYDGGRFALKEIPGVDLVVNGHIHTPKPPQQQGRTTWLNPGSIVRITRS
                     LHTLRIRPAATLWFPARDELQTLEIPHRPFDEVFPPWGEEQEANAPCPDESLFIKGLE
                     NLTLRKTTEGVGLQTFLEANLRKDDPIDTIIWELYEEVIPHEAD"
     gene            1652..1984
                     /locus_tag="PLM79_05950"
     CDS             1652..1984
                     /locus_tag="PLM79_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85885.1"
                     /translation="MKQTKKVGVPDAMGEDAKVQERLERLRAEYARLNEQRIATDRDR
                     KNLEEQLRGLRQKAEQEYGTSDVGELRDLLEKRRKENEQMVSQYEEHIREIKEKLQEI
                     ENPESQEG"
     gene            1990..2667
                     /locus_tag="PLM79_05955"
     CDS             1990..2667
                     /locus_tag="PLM79_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85886.1"
                     /translation="MNNVPVPTLSQGVEEVRRQFSRLLWQKDNLKKRLEETRKAIEET
                     DRFLELQPRVAERLEALCRELFGEILDEVERNLSYALQEVLGQDLRVVTSRDVQRGKV
                     TITFGMERGGRPEDILTGQGGSVCNILSVGLRLIALSQLDEKQHRRFLILDEQDCWLR
                     PDLVPQLMGIINAIARRLRFQILVISHHDVSRFREYADLIYRILPERDPGEGASLELL
                     EKAPGSP"
     gene            2726..2911
                     /locus_tag="PLM79_05960"
     CDS             2726..2911
                     /locus_tag="PLM79_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85887.1"
                     /translation="MKDESTLMYCPYCGRPFREHPLWVGGACIFCLADDSRQAAPQAF
                     CARCGALLEEGHSCPRE"
     gene            3465..4100
                     /locus_tag="PLM79_05965"
     CDS             3465..4100
                     /locus_tag="PLM79_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOV85888.1"
                     /translation="MGELQSVAGTIALTMGVGWASGINLYASIFILGLLGATGNIVLP
                     PGLLVLAHPAVIVASGFMYLLEFVVDKVPGVDSGWDALHTFIRIPAGAILAAGAVGKT
                     DPSIALAAAIFGGGLAAGTHAVKSGTRVLINTSPEPFSNWVASVGEDFIVIAGLWTAL
                     HHPYLFLLFLAGFIAFMIWVLPKLWRGIKKLFGFLANLLGRRRMDGRNPPQ"
     gene            complement(4173..4898)
                     /gene="pyrF"
                     /locus_tag="PLM79_05970"
     CDS             complement(4173..4898)
                     /gene="pyrF"
                     /locus_tag="PLM79_05970"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOV85889.1"
                     /translation="MTKKQVPLEERIIFALDVDSPEAAQKWVERLESHVRFYKVGLQL
                     FLAGWFHIIDWILGRGHRVMVDLKFYDIPETVSLAVRQLQGRGVSFATVHGNDPIIQA
                     AVKEKRDLKILAVTVLTCFGEEDMIEMFGQPANIEELVFSRARRALNIGADGIVSSGL
                     EAQRLRENLGDKFLIVTPGIRPGTNRQIPMDDQKRIVTAWEAMANGADHVVVGRPIST
                     AADPIAVVEQMQKEILRELERRK"
     gene            complement(4924..5799)
                     /gene="rsmI"
                     /locus_tag="PLM79_05975"
     CDS             complement(4924..5799)
                     /gene="rsmI"
                     /locus_tag="PLM79_05975"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOV85890.1"
                     /translation="MPSKPANEPGDKKTPGTLFLVGTPIGNLSDLSLRALDTLRQVDG
                     IAAEDTRRTRKLLSHYDIRKPLISCHAHNEVQRSAELLQRLIEGEKLALVTDAGTPGI
                     SDPGAYLVGRAIEAGIPVSVIPGPAALIAALAASGLSTHPFAFLGFAPARGPNRRKFF
                     ASHASLPMTLVLYESPKRLCRTLEDILDWWGDRKVCVARELTKIHEEFFRGRIREAVE
                     RYRVEPKGEITLVVSGFEGKEGPAREETQWRARLREILLESGMGVGEASAIIAREYGL
                     PKRVVYREALKQRES"
     gene            complement(5777..6142)
                     /locus_tag="PLM79_05980"
     CDS             complement(5777..6142)
                     /locus_tag="PLM79_05980"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOV85891.1"
                     /translation="MDSRREKGRLGEHLARELLRENGLKILESNVRCPLGEIDVVARD
                     GSTLVFVEVRSRSGRRFGLPQESVNRRKQRRMTQLAQWYLKGHPSPGLCVRFDVVAVS
                     WEQEPPRLTWIPNAFEACE"
     gene            complement(6199..6822)
                     /locus_tag="PLM79_05985"
     CDS             complement(6199..6822)
                     /locus_tag="PLM79_05985"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOV85892.1"
                     /translation="MSRRTSETPTVDMQCFERQAHSRGFRLVAGIDEAGRGPLAGPVV
                     AAAVILPPRADFPGVTDSKLLPGKKREELYRTILDQADVGIGWVEAPEIDRTNILRAS
                     FQAMILAVENLPRTPDFLLIDGPYRLPLSIVQEGIPHGDRRSLSIAAASIVAKVSRDR
                     KMNEYHQAYPVYGFDRHKGYGTREHLEAIRLHGPCPLHRRSFRGVLS"
     gene            complement(6822..7166)
                     /gene="rplS"
                     /locus_tag="PLM79_05990"
     CDS             complement(6822..7166)
                     /gene="rplS"
                     /locus_tag="PLM79_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOV85893.1"
                     /translation="MGVVLENLDRENMRADIPAFRSGDTVKVHVKIREGDKERIQVFE
                     GVVIRHHRGRMGATFTVRKVSYGVGVERIFPLHSPQIDKIEILRRGRVRRSRLYYLRQ
                     RVGKAARIREKR"
     gene            complement(7215..7799)
                     /locus_tag="PLM79_05995"
     CDS             complement(7215..7799)
                     /locus_tag="PLM79_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOV85894.1"
                     /translation="MGLQRAGFYMALVHYPVLNRKGEVIASALTNLDLHDLARAARTY
                     DVPVCYIVTPLNDQQALAGRLIRHWCEGIGKTLLPNRAEALERLRVVEHVGDAVLEIE
                     REWGKSPTVWATSAREQEAALSHEAARRILERSETPHLLLLGTGWGLAPGALGRADAL
                     LDPIRGVDGYNHLSVRCAAAILLDRLLGNRGGDR"
     gene            complement(7819..8559)
                     /gene="trmD"
                     /locus_tag="PLM79_06000"
     CDS             complement(7819..8559)
                     /gene="trmD"
                     /locus_tag="PLM79_06000"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOV85895.1"
                     /translation="MIFDILTLFPEMLESPLDHSILGKARERGLIRVRTFNIRDHAVD
                     KHQMTDDRPFGGGEGMVMKPEPLVAALESLRREEPPPWVVLLSPQGRLFRQEVAWKLA
                     ELPRLVLVCGRYEGVDERVARHFVDDILSIGDYVLTGGELPAMVVVDAVARLIPGVLG
                     NQASTAGESFAQELLEHPQYTRPRDFRGLRVPDVLLSGNHAAVRKWRRGQALLRTKRF
                     RPDLFARIRLTAEDRKLLEEAEGRPDEA"
     gene            complement(8549..9076)
                     /gene="rimM"
                     /locus_tag="PLM79_06005"
     CDS             complement(8549..9076)
                     /gene="rimM"
                     /locus_tag="PLM79_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOV85896.1"
                     /translation="MVHDRRMVAVGRITRAHGVRGAVRIHPYGETLGRQGPGDKLFLP
                     PAPGAALTELTILHLRSHGNVCIVRTKELVCGDAAQALAGTEVFLPEDALPEPEESEY
                     YYFQLMGLAVETAEGKPVGVLRDILPTAGHDVYVVESDKGEVLIPAVQEIIQDIDLAA
                     GKMVIDPPDGLIHDL"
     gene            complement(9076..9309)
                     /locus_tag="PLM79_06010"
     CDS             complement(9076..9309)
                     /locus_tag="PLM79_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOV85897.1"
                     /translation="MLKELIEYMARALVDNPEKVKVSEVEGEQTSVIELRVAKEDLGK
                     VIGKQGRTARAMRTILSAASTKIRKRAVLEIIE"
     gene            complement(9354..9605)
                     /gene="rpsP"
                     /locus_tag="PLM79_06015"
     CDS             complement(9354..9605)
                     /gene="rpsP"
                     /locus_tag="PLM79_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOV85898.1"
                     /translation="MAIRIRLARRGRTKRPFYKIVVASSEAPRDGRFIESIGSYNPLP
                     EQAEVNIKTERLEYWLQQGAKPTDTVRSLIRKFGVHAAS"
     gene            complement(10045..11400)
                     /gene="ffh"
                     /locus_tag="PLM79_06020"
     CDS             complement(10045..11400)
                     /gene="ffh"
                     /locus_tag="PLM79_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOV85899.1"
                     /translation="MFENLSDKFQKIFKHLRGQGKLTEQNIRDSLREVRLALLEADVH
                     YKVAKDFVAGIAERAVGQEVMQSLTPGQQVIKIVHESLKDLMGGSAEPLHFIGKPPIG
                     VMLVGLQGSGKTTTAAKLARRLVQEKRRPCLVPADIYRPAAIDQLITLAGQLQLPVYP
                     STVKQTPEEIVREAILHARQHNCDTLLVDTAGRLHIDRELMAELQRIKKILEPTEILL
                     VADSMTGQDAVQVAEAFHEALRITGVVLSKMDGDARGGAALSIRSVTGCPIKFIGSGE
                     KLEALEVFHPDRMSSRILGMGDVLTVIEKAQEAFDEKQAQELARKFREDTFTLEDFRN
                     QLRQVKKLGSFDQILGMLPGMGMLKELKKAKIDDREFVRMEAIINSMTREERKNAQIL
                     NASRRRRIADGSGTSVPEVNRLLKSYEEARRMMRQVMGGSTGTKKKKGKGRRGRAFFP
                     F"
     gene            11760..14117
                     /locus_tag="PLM79_06025"
     CDS             11760..14117
                     /locus_tag="PLM79_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85900.1"
                     /translation="MAQPCDEGPRAENRSSGDFRGRPDEEVLQTLFENLPNPVMITDF
                     EDRIVYLNPAAVVHTGCSDYRTTPRFGADFLRLEKDPHGSRPSSESLDGLPPADAPGY
                     LKGKDGSWHPATAGGFLLGGAHGQSPGRLTVLKIRHPEPTSDPDTGPGLHAPFWNPES
                     SFPVRDPHAMLKSILDYFPMPFFTVDRHLVVTSMNQHLEELSGFRREEVLYRMTCAQL
                     LATPECNTESCVLRQTMENRSPLVGVERWMKDREGRTIPIRVFCAMITDREGRVIGGF
                     AALRDITFMVEAEQRIRLLIEMSQEGILMADESRRILYVNSKMAEMAGEESDRLVGTR
                     LDKLLSDEFMELMDRQLAQTEDGGRQHLCFCSTFQPLKTPSLDYLALETCIAVSRVGR
                     TRVTCLYFRDLTKHIEFERQLRSANDFLNSIIRSSVDGIVVLDNKGKVVIFNEGAERI
                     LGYKAEEVMGSPELFHRIYSSEQARENMRRMRSNEYGPPGKLSATRVSLVRKDGEIVP
                     ANFSAAIIKVGDREVGTVGIFSDLREVLRMRRELEEARTQLIQAEKILSLGRLAAGVA
                     HEINNPLAGILIYADMLLEDFPPDSPWRPDLEEIVRQTLRCKEIVMRLLEFSRQPLGK
                     RLPYDMNEVIGRCVELLRNQSMFLNIDFCLDLEKDIPPVHGDPGQIQQVFTNLILNAA
                     DAMGGRGRCTITSGYSPSTRTVLLKFMDTGPGIPKENLDKIFEPFFTTKPPGQGTGLG
                     LSVAYGIVQQHGGNIEVGNLPDGGAVFSIHLPLEPPEYQSEILEG"
     gene            14124..14504
                     /locus_tag="PLM79_06030"
     CDS             14124..14504
                     /locus_tag="PLM79_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85901.1"
                     /translation="MSTVLVVDDEKVIREGCSRALSRAGFHVLLACNGKEALDVLAST
                     PVDVILCDLKMPVMSAVELLLQVSADHPSLPFIIITGHGTISDAVECMRKGAYDFVTK
                     PFRVEVLLRTVDCAARGLPPPPKV"
     gene            14614..16086
                     /locus_tag="PLM79_06035"
     CDS             14614..16086
                     /locus_tag="PLM79_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85902.1"
                     /translation="MGTILVVDDEKVIRDGCSRLLSSEGHRVLTASDGKEALEILESE
                     AVHVLLCDLKMPVMDGIEVQKRVRVEYPELPFVVITGHGTIDTAVECMKNGAYDFITK
                     PFRTDHLQFIVRRALEKRELEEKTRVLREAHERNLYDLAAEQSRVRTIVHCMAEGVLV
                     TNRDLEVVLQNPALARLLDLPKVPEQPTLLSRYLEEPFLVEAIEGLMERARDRWEMVS
                     REFRKGSLDLRALSAPIFGGAGEVLGSVTVFQDITSFKELDRMKSHFVQMVSHELRSP
                     LATVLQQMGVILEGLAGELNEKQRSLLDRSRDKIQSLLDLINDLLDVAKIESGHAVQE
                     QVPLNLGEVLSEVAAFFRERAQRQAIAVELEIHPHLPLIHADRRCMEEVFTNLVSNAI
                     QYSPDGGRVRLCAMPCGENVEVRVTDTGIGIPSEEIPKIFDKFYRVKHPRTRQIIGTG
                     LGLAIVKGIVESHRGSVEVESELGAGTTFRLLFPVMETRG"
     gene            16096..17457
                     /locus_tag="PLM79_06040"
     CDS             16096..17457
                     /locus_tag="PLM79_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85903.1"
                     /translation="MAELKNRHAVLIVDDESAVRNGIARVIEKKGYDVRTADSGESAL
                     ELAARDPFDVVFLDIRLPGMDGMEVLRQLRAKNPETAVIMITGYPTIDTAIESLRLGA
                     LDYLVKPFRVDDLEVMLEKALESAVSDGKEPDPELDVGRDIPSLIGKSPAMRDVFLKI
                     KRAAPSDSTVLITGESGTGKELVARAIHNLSLRADKEFVAVDCSALVETLLESELFGH
                     VKGSFTGAFQTKHGLFELANRGTFFFDEISNLSPNIQAKLLRVIQEREFMKVGSQKRI
                     KLDIRIIASSNRDLREATAEGTFREDLFYRLRVVPIRLPPLRERVEDIPLLAEHFIEK
                     YSRKGNRAVQGISREAMDLLCSYSWPGNVRELEHCIERILILEDGEIIRPENLPAFIT
                     QRQGEFRIFSEEVFSLKELEKRYIQFILRRTKGRKQETAQILGIDRKTLSVKIKKYGI
                     KTG"
     gene            17482..18228
                     /locus_tag="PLM79_06045"
     CDS             17482..18228
                     /locus_tag="PLM79_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV85904.1"
                     /translation="MNSHPILSLSVLGVLFLLVPCFSPVQAMGKDTIATGSGPVEVGF
                     IGHGSLVFFFQGKTVYIDPVAAYGDYSALPKADLILLTHEHKDHLDPQTLSLIRKKDS
                     VLIVTRACADRLKEGTVLENGDVKEVLEISIRAVPAYNLVHMRSEGQPYHPRGRGNGY
                     VLTFGDKKIYVAGDTENVPEMKALRDIDVAFLPMNLPYTMTPEMVADAAAAFRPKILY
                     PYHYGDTDPQRLRDLLKDEKDIEVRVRTLR"
     gene            18290..18634
                     /locus_tag="PLM79_06050"
     CDS             18290..18634
                     /locus_tag="PLM79_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV85905.1"
                     /translation="MTLEDLYELIPDFPCLPGCTECCRVSAPPSRTPGEQVRLIEYVQ
                     SHAVRLDPREEGCPYRSDDGCLVHPVRPFICRLYGTSPGYLCRMGIRPVRLLHEDEEA
                     DLFHFYYTHFAP"
     gene            18680..19501
                     /locus_tag="PLM79_06055"
     CDS             18680..19501
                     /locus_tag="PLM79_06055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV85906.1"
                     /translation="MKTHSIAEIFPPSPGKVRLQTLTRNLTAGLLSCLVLWSCTAASS
                     QPSRPSPSPPPTSFSHPPQERTPVQSPVTDRGSTYRVVTEEELIQWEQTDPGLSFNRC
                     REILSRLNIKAGYHISDDLRRGRALLAPNDFRAFKDWTPLPCHAPKLAEFPKAILVVK
                     EIPFLGWYENGFLTGDAPACIGPPGEDTRAGRFVVIEKDADHKSRSYRNDLGEPAWMP
                     WALRVYDTVWIHAGDVSGKHCSHGCIILPVKTAERLFHWADLETPVVIVESLSDL"
     gene            20285..21910
                     /locus_tag="PLM79_06060"
     CDS             20285..21910
                     /locus_tag="PLM79_06060"
                     /inference="COORDINATES: protein motif:HMM:NF033874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidJ-related pseudokinase"
                     /protein_id="HOV85907.1"
                     /translation="MTGTSRAGCAERATEGSLLGKRIRERIADFSAVYMHVTELRQWL
                     RDGRRRVGSEMVEILVSVLDEPTHRSQKQVFFLHREAAGVLAELIGDPGLPVEVRALA
                     LSRLKRLAASSVQSVHRAVCEALGSLPLDIRGPLISPNGDGEVSRLGGWELSRALASQ
                     GCGPPYRVGRSFVSSGTDSSFVVSIKVWQGPDAHTAAVREWTWMELLNRVKPSIPKRF
                     DIPEPLLAATTPVFQLTSFPDACGNETPLAENRCILFRAPRDYYRYPNGSAVSRLLDE
                     SEFLEVILRNAWLFGKTASMGIVHTAPIPLFHNRTQVDRRNDGGIYQWPRGGRLDRWL
                     ESCRHPNFGISGLRDFEHLRSLRTAGDGRGFYQDIGTQILSLVLVAGSYFRGKAPQRV
                     GLDSRGKPVDARDLFHRPLLEDLLHGIYREYHRGLVGAPPREPVPLDVSHLAERMIEE
                     MGVDRHMEEIFRVADQERMSQAEFEGFLAERGCSRQEIGRRLKGAEDIALQTGPHLGR
                     FNGPISLPEMLRFVAVTAAGCIADRFMRSNLCP"
     gene            22040..22879
                     /locus_tag="PLM79_06065"
     CDS             22040..22879
                     /locus_tag="PLM79_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85908.1"
                     /translation="MTSHKGVRFRSHYLFRSVLVAGVLLCFAAFSAEVQGDRNGLYQL
                     YLKAVDDAKTVNPHKISRNLVAITKGNPNLPLRWEGVPEKSRVLVVTWTDKDYYDDYV
                     GRDYVLPPSANVWVTAVPELKEFVRKHVVPVPPLRVEQLLGLPPHTGKTKFVEIWADR
                     KSLFRPSPDPEITDHEAELRFPTKVNPFLKYDPGASMIEWNSDLQKEVKYTYPEWFNH
                     RKRTIYTGDSPYPWTRLGYTYDWGNVQTRVGLSEFVILGGSTVGIHAVRSNAEYLNPA
                     LLN"
     gene            23320..23697
                     /locus_tag="PLM79_06070"
     CDS             23320..23697
                     /locus_tag="PLM79_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85909.1"
                     /translation="MAATLGTLLIAEPGCRLVSALKPWLLHQGYKVKAVEDMKGLLRT
                     LQCENVRVLVLDVCLAESMDYEAISIIKGLCRNLPIIVTTESNNPEQESSIRQKGIFY
                     YHVRSFGVEELVLAITNAMTRHP"
     gene            23791..24666
                     /locus_tag="PLM79_06075"
     CDS             23791..24666
                     /locus_tag="PLM79_06075"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV85910.1"
                     /translation="MSLEDKLTSGKFLVLAEMDPPKGVDVTGLVANAFRVKGKVDAYV
                     VPEMSNAVMRMSSLGGCMVLQSKGLETVLQVCCRDRNRLALQADLLAAYSLGISTLMA
                     VAGVDPGYGDHHRTLAVNDINLMELLQGIRTLQEGKDMAGIELAGAPKFCVGSTTNAG
                     TIEGPLEAEIEEMEKKAAVGVRFFVTPPVFDIQPLQRLLRATADRGMKIVPTVLLLKS
                     AGMAGYINRHIEHVSVPSELIRRIQKAPDRPRECVRIAAELVSAIRRTRAAGVLLSTI
                     GWEDKLAQILEGVET"
     gene            24663..27725
                     /locus_tag="PLM79_06080"
     CDS             24663..27725
                     /locus_tag="PLM79_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV85911.1"
                     /translation="MKREEEAPVGSVLVVGGGVAGIQASLDLANSGFFVTLVEKSPAI
                     GGVMAQLDKTFPTNDCSMCILSPKLVECGRHPNIRLLTLADLVDLDGGVGRFTATVRR
                     RPRYVNLDKCIACGVCAEKCPRKVPDEFNERLGNRKAAFVKYPQAVPLKYAVDREHCI
                     YFEKGKCRACEKFCPAGAVELQQEEIREKIEVGSVILAPGFQAFSPTRYEYGYGRHPD
                     VVTSIEFERILSASGPFQGHLRRPSDGKVPRKIAWLQCVGSRDLQHGAHGYCSGVCCM
                     YAVKEAVVAKEHASDPLDAAVFFMDMRTYGKDFEKYFLRARDEYGVRFVRSRVHTVDP
                     SPTGDCLLIGYVDEGGAVFEEEFDMVVTSVGLEIGKDTEDLARRIGVELDETRFVFPR
                     EFAPVATSRKGVYACGAFQGPKDIPASVMEASAAACAAAMDLAASRGSRVEVPPAVPE
                     EDLAGEEPRIGVFVCNCGINIGSVVDVNEVTAYAAGLPHVAATCQNLFTCSQDTQERI
                     KEVIRDKKLNRMVVAACSPRTHEPLFQETLRACGVNKYLFEMANIRDQDSWVHPADPR
                     KATDKAKDLVRMAVARASLLQPLEERPLSIKRRGLVIGGGVAGLNAALCLARQGFEVV
                     LIEREKTLGGMARQIAQTIEGWDVQEYLDHLVAEVQHHEKIQVLTETLVVGFGGYKGN
                     FTTEVLVGPGPYERKIEHGVTIVATGALEYRPREFLYGDHPRVMTQLELGRQLRNNPL
                     EIGRWENLVMIQCVGSRNEEHPNCSRVCCQSAVKHALHLADLNPDMQITILYRDVRTY
                     GLLEEYYKSARGKGILFVRFDADRPPRVTGDGEGLCVTFRDHVLDRPIRMKTDAVVLS
                     AGMIAPDTEELASFLKVPRNADGYFIEAHAKLRPVDFASEGIYLCGTAHGPKLIQEAI
                     AQAMAAAARAASFLASSHMTYGGVVARVQPELCAACLVCVRRCPFGIPRINEHGVSEI
                     NEALCQGCGVCVSECPAKAIHLSHYEDDQIMVKVDALFETPERDYESV"
     gene            27712..28149
                     /locus_tag="PLM79_06085"
     CDS             27712..28149
                     /locus_tag="PLM79_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:27757..27759,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV85912.1"
                     /translation="MKAFEPVIVAFCCNYUAYSAADLAGSMRLHYPTNLRIVKVPCTG
                     RVAAIHLMKSLEKGADGVLVAGCLEGDCHFQSGNLRARKRVDYVRRILETIGIEPERV
                     AMYNLSAGMGPRFAEIALEMTEKVRQLGPNPVKAAKGGKESAA"
     gene            28146..28826
                     /locus_tag="PLM79_06090"
     CDS             28146..28826
                     /locus_tag="PLM79_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV85913.1"
                     /translation="MIMAKPKPLQEIIGYVRTLHKLLVVGCNGCVAVCEAGGLKEVEV
                     LSSALRMHFMKEGKVLEILEMSLTRQCDKEYLEELKERIDGYDAVLSLACGAGVQLLA
                     EMYENKPVFPAVDTCFIGVTEEQGMWSERCQACGQCVLSETAGICPITRCSKRMLNGP
                     CGGSENGKCEISILVGRDVACGWHLIFERLKKSGRLDSFRRPAPDKDWTLSRDGGPRR
                     IIREDVRP"
     gene            28823..29794
                     /locus_tag="PLM79_06095"
     CDS             28823..29794
                     /locus_tag="PLM79_06095"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV85914.1"
                     /translation="MTSTTPSKLEKILAAGHLAVTSECGPPRNGRGDVVEKKGELLRG
                     FVDAINVTDNQTAVVRQCSLASCIRLKLMGLEPVLQMVTRDRNRIAIQSDLLGAASFG
                     IHNVLCLTGDHQRFGDHPNSVNVFDMDSTRLIQTVKTMRDDGKLLSGHPMEEGRPQMF
                     IGAAENPFADPFEVRVLRLAKKVAAGAQFIQTQCIYNMEKFKLWMKMVRDEGLHEQVA
                     ILAGLTPMKNVGMARYMKNRVPGIDVPDPIVKRLAGVPKENQAEEGIRICLEQIEELR
                     GEEGIRGFHIMAIEWEEKVPEIVKRAGLYPRPAADEPGGLPGGTTGS"
     gene            30012..30395
                     /locus_tag="PLM79_06100"
     CDS             30012..30395
                     /locus_tag="PLM79_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV85915.1"
                     /translation="MSKKYVLVVDDDPDFVETVGMMLESKGYEVGKAYDGIEGEQSIL
                     ARKPDLLVLDVMMPRKNGYTLCAELKKSRDTRDIPIVMLTAVGEAVPTTSYSHADGMA
                     MEADDYIPKPVDTQALINAIERLFS"
     gene            30850..31863
                     /locus_tag="PLM79_06105"
     CDS             30850..31863
                     /locus_tag="PLM79_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85916.1"
                     /translation="MTKVRLGGIKVLAGRSHLTNLCPADESALHDVCRRMAADGINLS
                     FLTHLCSDHEGNCYTAACTEIADAYSGYFHWKAGGGSCHVGNPEPDVNILSIFPHNQN
                     PLIAGTLIQLIARQRTQPRGFASSPSAISVLIRGADTSKAIDGLFDLFEFPTHRSPLD
                     WHAAYEGQEHVLKEIICSFQEDVIKVYDFARQAGFELWNFTVSGDGLEQLGKFLTALG
                     KNQARSPFLVGHPDTRNRTHFSICLPEPSRPFIEESLSAQPFAEAASCAGSVAVVSLH
                     GPHFGDRFGIVHALLRSLEQGRILPLALCCVVSTISLVVAADELPDTLRAIETSFQIP
                     HSG"
     gene            complement(32370..32603)
                     /locus_tag="PLM79_06110"
     CDS             complement(32370..32603)
                     /locus_tag="PLM79_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85917.1"
                     /translation="MASTATMATMMKMLESLPEQIQERVLEHMREYIEDIRDEAKWDE
                     SFTRTQTKLVAAARQARKEIAEGKASPMDRKRL"
BASE COUNT         6484 a        10131 c         9704 g         6336 t
ORIGIN      
        1 tcctggagtc catggatcct ccccccgatc ttcaggacgt cgaacccttg gccgggctcg
       61 tccaggagct gggccgaaac cgagatcgct gggcccggtg ctcaaccttg tgccggggat
      121 tggaacctct cagggaccct ccggagctcg aagccgtgcg cgaccttcat gccgtcgtgt
      181 cctccctcga ggaattgacc cggagccaat ccaggatcca cgttcgcagc tcccggcttc
      241 gatccctgga agagcccccc gaggcatggg acccgactcc cctgaaggac ttgatccggg
      301 tattggagga cctggaaatc ggctgccgcc ggacccgaaa acgctgcgag acgacggaaa
      361 gactgcagcc ggttcctaca ccgggcgata cccgggacct ggaggaaacg gttcttgccc
      421 tcgaaaaaac ccgggaccac ctgaaacgca ccggggatcg cttgtccctc cttcaggcaa
      481 cggagccgtg cccccagctg caccctctca aagacctcga tgagtggatc gccgaagaaa
      541 gccggcttca aaacgagcaa tcccggctgg aacggcggca cgccgaatgc ctgaaggcga
      601 tccaaagaaa gaaaaaggag atcgaggggg tcctcaggga gatcggggcc tgtcccctat
      661 gcggccaagc cttcgacctg acccacttcc tggagaaaga ccatggttga gaccgaccgc
      721 accccctcga agccctacca gggggttctc ttcattggag accctcacct ggccgctttt
      781 cctccgggtc atcggctgga cgattacccg caaacggtcc tgggcaagct ggcatattgc
      841 ctggaggcgg cggggaggca tcgttgcctg ccggtaatcc tgggcgacct cttccatgtg
      901 ccccgcaaca accccaattc cctcctggtg gacctcatcg agcttttccg gggttcccgg
      961 ccctgggtgc tggtgggcaa ccacgacaaa cacgaggccc gcctgacccg ggatgtctcc
     1021 ctgtcggtcc tcgaagccgc gggagtcatt cgcctcctca aggaaagcgg cccggcggat
     1081 tccgtccttc tgggaggcgc cagggtcctc atcggcgcat cccccgactg gactcccctt
     1141 cccaaagagg tcgagccccg ggaccatgat cgcgtcctct ggctgaccca tcacgacctg
     1201 gccctgcccg gctacgacgg gggcaggttc gccctgaagg aaatcccggg ggtggacctg
     1261 gtggtcaacg gccacatcca cacccccaag cctccccagc agcaggggcg caccacctgg
     1321 ctcaatcccg gaagcatcgt tcggatcact cgaagcctcc ataccctgcg gattcggccc
     1381 gcggcaactc tgtggtttcc ggcgcgggac gaactgcaaa ccctggaaat cccccatcgc
     1441 cccttcgacg aggtcttccc tccctgggga gaagaacagg aggcaaacgc cccgtgcccg
     1501 gatgaatccc tcttcatcaa ggggctcgag aaccttaccc tgcggaaaac caccgaaggg
     1561 gttggtcttc aaacgtttct cgaagcaaat ctccggaagg atgaccctat cgacaccatc
     1621 atctgggaac tgtacgagga ggtaattccc catgaagcag actaagaagg ttggggtgcc
     1681 ggacgcgatg ggtgaagacg caaaagtgca agagcgtctg gagagactcc gagccgagta
     1741 cgcccggctc aacgagcaga ggattgccac ggaccgggac cgaaagaacc tggaagaaca
     1801 gctccggggc ttgaggcaaa aggcggaaca ggagtacggc accagcgacg tcggggagct
     1861 tcgcgacctg ctcgagaaac gccgcaagga gaacgaacag atggtttccc aatacgaaga
     1921 gcacatccgg gagatcaagg aaaagctgca ggaaatcgag aaccccgagt cccaggaggg
     1981 atgaagcgga tgaacaacgt tcccgtcccg acactctccc agggggtcga ggaggttcgg
     2041 cggcagtttt cacggctgct ctggcagaaa gacaacctga agaaaaggtt ggaggagacc
     2101 cgcaaggcca tcgaggaaac ggaccggttt ctcgagctgc agccccgggt ggccgaacgc
     2161 ctcgaggccc tctgccggga gcttttcgga gagatcctgg acgaggtcga acggaacctg
     2221 tcttacgccc tgcaagaggt cttgggccag gacctgcggg tggtgacctc ccgcgacgtt
     2281 cagcggggaa aggtgaccat caccttcggg atggagcggg gcggacgtcc cgaagacatc
     2341 ctgaccggcc agggcggatc cgtgtgcaac attctctctg tgggacttcg actcatcgcc
     2401 ctctcccaac tggatgaaaa acagcaccgg cgctttctca tcctggatga gcaggactgc
     2461 tggctgagac ccgacctcgt cccgcagctc atgggcatca tcaacgccat cgcccgcagg
     2521 ctccgtttcc agattctcgt catcagtcac catgacgtga gccgttttcg ggaatatgcc
     2581 gacctcatct accggatcct gccggagcga gaccccgggg aaggagcaag cctggagctg
     2641 ctggaaaagg ccccgggcag cccataaagg ggggtctttc ggcaaccggt cggcggatgt
     2701 ttcgggaaag gaaggaaaag gccggatgaa ggacgaaagc acactcatgt actgtccgta
     2761 ttgcggaaga ccgtttcgag agcacccgct gtgggtgggc ggcgcctgca tcttctgcct
     2821 ggcagacgat tcccgacaag ctgccccgca ggctttttgc gccaggtgcg gggcactcct
     2881 cgaggagggc cactcgtgtc cccgggagtg aatcccgcca aggcggcggg tgatggcggg
     2941 atctcttccc gtcatggcgg gatttcccct tgcgtggcgg gattcacgac ggaacccgaa
     3001 ttcgcgtctt ccccgggtga attcgtaact cattgtaatt gcatgtaaat aaatctctat
     3061 cgccctttgg cacggcgttt gctcttgcat aagaccgaac gtacaaatct cttttcctcc
     3121 taaaagccga acggtccgcc ccaccgagcg gcttttttgt ttgtaaaccc tccgagcacg
     3181 tctccgagga ttttcgaggc cgttcccagg gatcaggccg gtcgcttttg gcaaggaagt
     3241 gtggcgggtc ccccatgcag atctcacatc tccttggggt tccgggagtt gcgggcttcg
     3301 cccggcggtc tgtctgggag cgaaggggaa aggaagggcc tccggggagt tccggacccg
     3361 ggaactcgtg cccatctcgt catcccatgc gacttttcgg ttgattcggc ccccggcaag
     3421 gcctatgttc gggggaccac cattctggtt gaaagggcgg acctatgggg gagcttcaga
     3481 gcgtggccgg gaccatcgcc ctgacgatgg gcgtcggatg ggcgagcggg atcaacctct
     3541 acgcctcgat cttcatactg ggtcttttgg gggcgaccgg aaacatcgtt ctccccccgg
     3601 gtctgctggt tttggcccat cccgccgtca ttgttgcgtc ggggttcatg tacctgctcg
     3661 agttcgtggt ggacaaggtc cccggcgtgg attcggggtg ggatgccctg cacactttca
     3721 tccgcattcc cgccggtgcg attcttgccg ccggagccgt ggggaagacg gacccgtcca
     3781 ttgcccttgc ggcagccatt tttgggggcg ggcttgccgc cggaacccat gccgtgaagt
     3841 ccggaactcg ggtcctcatc aacacctctc ccgaaccctt cagcaactgg gttgcctcgg
     3901 taggagagga cttcatcgtg attgcgggac tttggaccgc ccttcaccac ccttatctct
     3961 tcctgctgtt cctggcggga ttcatcgctt tcatgatttg ggtccttccg aagctgtggc
     4021 gagggatcaa gaagcttttc ggctttcttg caaatctttt ggggcgtcgc aggatggacg
     4081 gccgaaaccc gccacaatag catgtgcctc ccggccgaag gggttgggtc tgcaggcctg
     4141 tcggccggga aacctttgtc aaggtccgcc tgttacttcc ggcgctccag ctcccggaga
     4201 atctcctttt gcatctgttc caccacggcg atggggtccg cggccgtgct gatgggtcgc
     4261 ccgaccacca cgtgatcggc cccgttggcc atggcttccc aggccgtaac gatccgtttc
     4321 tggtcgtcca tggggatttg gcggtttgtt cccgggcgta tgcccggggt cacgatgagg
     4381 aacttgtctc ccaggttttc gcgcagtctt tgcgcctcca gccccgagga aacaatgccg
     4441 tcggccccga tgtttagtgc ccgccgggcc ctcgagaaaa ccagttcctc gatattcgcg
     4501 ggttggccga acatctcgat catgtcctct tccccgaaac aggtcaacac ggtcaccgcc
     4561 aggatcttga gatctctctt ctccttgacc gccgcctgga tgatgggatc gttgccgtga
     4621 accgtggcga aggaaactcc ccggccctgg agttgtcgaa cggccaggga gaccgtctcg
     4681 ggaatgtcgt agaatttcag gtccaccatg acccggtgtc cccgcccaag gatccaatcg
     4741 atgatgtgaa accacccggc caggaacaat tggagtccca ccttgtagaa ccggacgtgg
     4801 gattccagcc gttccaccca cttttgggcc gcctcggggg aatccacgtc cagggcgaag
     4861 atgattcttt cctcgagagg tacctgcttc ttcgtcacga tttcctcctt ccgggcgagc
     4921 ctgtcatgac tccctctgct tcaaggcttc ccggtacacg acccgcttgg ggagtccata
     4981 ctcccgggca atgatggcgg aggcttctcc gactcccatg cccgattcga ggaggatctc
     5041 ccggagtctc gccctccatt gcgtttcttc ccgagccggg ccttccttcc cctcgaatcc
     5101 cgagaccacc agagtgatct cccctttggg ctccacgcgg taacgttcca cggcttcccg
     5161 gatccggcct cgaaaaaact cctcgtgaat cttggtcaat tcccgggcca cgcacacctt
     5221 gcgatctccc caccaatcca ggatatcctc gagggttcga cacaaccgct tgggagactc
     5281 gtagaggacc agggtcatgg gcagcgaggc gtgggaagcg aagaatttcc gtcgattggg
     5341 tccccgggcg ggagcaaacc ccaggaaagc gaatggatgg gtggacaggc ccgaggccgc
     5401 cagggccgca atcagggctg caggccccgg gatgacggaa acgggaatcc cggcctcgat
     5461 ggcccggccg accaggtacg ctcccgggtc cgagattccg ggggtccctg catcggtaac
     5521 cagggccagc ttttccccct cgatcagcct ttgcagcagt tcggcgctcc tctggacctc
     5581 gttgtgggca tgacagctga tgaggggctt acggatgtcg tagtggctga gaagctttcg
     5641 ggtacggcgc gtgtcctcgg ccgcaatgcc gtcgacctgc cggagggtat ccagggcccg
     5701 cagggagaga tccgacaggt tcccgatggg ggttcccacc aggaagaggg tgccgggagt
     5761 tttcttgtcc ccgggttcat tcgcaggctt cgaaggcatt ggggatccat gtgagcctgg
     5821 gtggttcctg ttcccaggaa acggccacca cgtcgaaccg cacacacaac ccgggagacg
     5881 ggtggccttt caggtaccac tgggccagct gggtcatgcg tcgttgcttc cttcgattga
     5941 cggattcctg ggggagcccg aaccggcggc cgctgcggga acggacttcc acaaaaacca
     6001 gggtggagcc gtcccgggct accacatcga tctctcccag gggacagcgc acgttggatt
     6061 cgaggatctt cagtccgttt tcgcggagga gctcccttgc gagatgttcc ccgagccttc
     6121 ccttttcccg gcggctatcc atggcgacac tccaaagatt ggctcatcgg ttcgcggtgt
     6181 cgggcggctg ggcgatcctc atgagaggac ccctcgaaag ctgcgtcggt ggagcgggca
     6241 gggaccgtgg agacggatgg cttcgaggtg ttcgcgcgtc ccgtatccct tgtggcgatc
     6301 aaacccgtaa acggggtagg cttggtgata ttcgttcatt tttcggtccc gggacacttt
     6361 ggccacgatg gaagcggcgg cgatggagag gctccggcgg tctccgtggg ggatcccttc
     6421 ctggacgatg gagagaggca gccggtaggg gccgtcgatg agcagaaaat ccggcgtacg
     6481 cggcaggttt tcaaccgcaa gaatcatggc ttgaaaggag gcccggagga tgttcgttcg
     6541 atcgatttcg ggggcttcca cccagcctat gcctacgtcc gcttggtcga gaatggtccg
     6601 gtagagctct tcccgctttt tcccgggcaa aagcttggaa tccgtcaccc cgggaaaatc
     6661 cgcccgggga ggaaggatta cggcggcagc cacaacggga cccgccaggg gacctcgtcc
     6721 cgcctcgtca atccccgcca ccaaacgaaa accccggctg tgggcttgcc gttcaaagca
     6781 ttgcatgtcc acagtcgggg tctccgaagt tcttcgagac actagcgctt ctcgcgaatc
     6841 cgggcggcct tgcccacgcg ctggcgcagg tagtagagcc tcgaccgtcg aacgcggccg
     6901 cgcctcagga tctcgatctt gtcgatctgg ggagaatgga gggggaaaat cctctcgaca
     6961 cccaccccgt aagacacttt tcgcacagta aaggttgcgc ccatgcgacc ccgatggtgg
     7021 cgaatgacca ctccctcgaa aacctggatg cgttccttgt ccccttcccg aatcttcacg
     7081 tgcaccttga ccgtgtctcc gctgcgaaac gcgggaatgt ccgcgcgcat gttttccctg
     7141 tcaaggtttt ccaaaacgac tcccataaac aacctccatc atgctaatga ccggggtccg
     7201 atcccccgtc gatcctatct atccccgcca cggttcccga gcaatcgatc caaaagaatt
     7261 gccgcagcgc accgtaccga caaatgattg tacccgtcga ctccccgaat gggatccagg
     7321 agagcgtccg cccggcccaa tgccccgggg gccaggcccc aaccggtgcc taagagcagc
     7381 aggtgggggg tttccgacct ctcgaggatc cttcgagccg cttcatggct cagggccgcc
     7441 tcctgttccc gggcggaagt ggcccatacg gtcggggact tcccccattc cctctcgatt
     7501 tccagaaccg catcccccac gtgttccacg actctgagcc gttccagggc ttccgccctg
     7561 ttgggaagca gggttttgcc gatcccctcg caccagtggc ggatcaatct tcccgccagg
     7621 gcctgctggt cgttcaaggg ggtgacgatg tagcagacgg gaacgtcgta ggtcctggcg
     7681 gcacgggcca agtcgtgcag gtcgaggttc gtcagggccg aggcgatgac ctcccccttt
     7741 cgattcaaga ccgggtaatg gaccagggcc atgtagaaac cggctctctg aaggcccaat
     7801 tccgactcct cccctccttc aggcttcgtc cggccgcccc tccgcctctt ccagcagctt
     7861 tcggtcttcg gccgtgagcc ggatgcgggc gaaaaggtcc gggcgaaagc gcttggtcct
     7921 gagcagggct tggccccttc tccacttgcg gacggccgcg tgattgccgg aaagcaggac
     7981 atcgggcacc cgaagtcccc tgaaatcccg ggggcgcgtg tattgaggat gctcgaggag
     8041 ctcctgggca aaggattccc cggccgtgga cgcctgattt cccaagaccc cggggatgag
     8101 ccgggccacg gcgtccacca ccaccatggc cgggagctct cctcccgtca atacatagtc
     8161 gccgatggag aggatgtcgt ccacaaagtg tcgggccacc cgttcatcca ccccttcata
     8221 ccggccgcag accaggacca atcgaggaag ctcggccagc ttccatgcga cctcctgtcg
     8281 aaacaggcga ccctggggac tgaggaggac cacccatggg ggcggttctt cccgcctcag
     8341 ggattcgagg gccgccacca ggggttccgg tttcatgacc atgccttcgc cgcctccgaa
     8401 cgggcggtcg tcggtcatct ggtgtttgtc gacggcatgg tcccgaatgt tgaaagtgcg
     8461 aacccgaatc agcccccgtt ctcgagcctt gcccaggata ctgtggtcca ggggagattc
     8521 gagcatctcg ggaaataaag tcaggatatc aaagatcatg aatcaggccg tcaggcggat
     8581 cgatcaccat tttgccggca gccagatcga tgtcttgaat gatctcctgc acggccggga
     8641 tgagaacctc acccttgtcg gattccacca cgtagacatc gtgtccggcc gtggggagga
     8701 tatcccgcag gactcccacg ggctttcctt cggcagtctc caccgccaat cccatcagtt
     8761 gaaagtagta atactcgctt tcctccggct ccggaagcgc gtcctcggga aggaaaacct
     8821 ccgttccggc caaggcctgg gcggcgtctc cgcagacgag ttccttggtc cggacaatgc
     8881 acacgtttcc atgggatcgc aggtggagga tggtcaattc ggtcagggcg gcaccgggcg
     8941 cagggggcag aaaaagctta tccccgggtc cttgacggcc aagggtttct ccatacggat
     9001 gaattcttac ggcacctcga actccatggg cgcgggtgat cctgcccacc gccaccatcc
     9061 gacgatcgtg caccattatt cgatgatttc caacaccgcc cgttttcgaa tcttggtcga
     9121 tgcggcactc aaaatcgtgc gcatggcacg ggcggtcctg ccctgcttgc cgatcacttt
     9181 tcccaggtct tccttggcaa cgcgcagctc gatcaccgaa gtctgctcgc cctccacttc
     9241 cgaaaccttg accttttcgg ggttgtccac caaagctcgg gccatatact caatcaattc
     9301 cttcaacatg gcgacctcca gatctcatgc accgcacgtt caaccctgat tccctaagat
     9361 gcagcatgaa ctccaaactt ccgtatcagg cttcgcaccg tatccgtagg tttggcaccc
     9421 tgctggagcc aatactcgag cctttcggtc ttgatgttga cttcagcctg ttccggcaac
     9481 gggttgtaag aaccaatgct ttcgatgaat cgaccgtccc gtggagcttc ggaagatgcc
     9541 accacgatct tgtaaaatgg cctctttgtc cttcctcgac gagctaagcg aatgcgtatt
     9601 gccataatct gcgcgcaacc tcctgtccgt tgtgttttct ctaaatcgag cgattggcat
     9661 ttagccgttg gctttgaaat tatcagtggt tcagccgctt ctagccacca cccgactgtg
     9721 acgtccgaaa atacctgaaa tgcaatacca aagagctgac agccaaaggc aaacagctaa
     9781 ccgctcaatc taggttttca tgggcctttg gacggccggt cccttccgat gtcgccgtat
     9841 aggaccccgc atgacccggg acaaacgtcc atcccatggg gaaacgaaag cgacgtgacc
     9901 tgtttctttc aagggaatcg ccaaacagac tccggttcgg cgcccgtggc aactgccggt
     9961 gacaacctgt ttccaggcgg aacaagatcc tggttcgccg tcgtactgcc gtggaaagac
    10021 atcctatatc gtatttaccc ggaattaaaa agggaaaaat gctcgtcccc ggcgtccctt
    10081 ccctttcttc ttcttggtcc ccgtcgatcc ccccattacc tggcgcatca tcctgcgggc
    10141 ctcttcgtag ctcttgagca gccggttcac ttccggcacc gaggtgccgc tgccgtcggc
    10201 aatgcgccta cgccggctcg cgttgaggat ctgggcgttt tttcgttcct cccgggtcat
    10261 ggaattgatg atggcttcca tgcggacgaa ttccctgtcg tcgatcttgg cctttttcag
    10321 ctccttgagc atccccatcc ccggcagcat gcccaggatc tgatcgaaag agccgagctt
    10381 tttgacctgg cgcaactggt tgcgaaaatc ttcgagggta aaagtgtcct cgcggaattt
    10441 ccgggccagt tcctgggcct gcttctcgtc gaaggcttcc tgggccttct cgatgacggt
    10501 cagcacgtcc cccatgccca ggattctcga gctcattcga tcgggatgaa acacctcgag
    10561 agcctccagc ttttcccccg acccgatgaa tttgatggga catcccgtca ccgatcggat
    10621 ggacagggcg gctcctcccc gggcgtcccc gtccattttc gacaggacga ccccggtgat
    10681 ccggagtgct tcgtggaagg cctcggccac ctgcacggcg tcctgacccg tcatggaatc
    10741 cgccaccagg aggatttccg tgggttcgag gatcttcttg atgcgctgga gctccgccat
    10801 gagctcccgg tcgatgtgaa gcctgcccgc ggtatccacg agaagcgtgt cgcagttgtg
    10861 ctgccgggcg tgcaggatcg cctcgcggac gatttcctcg ggagtctgtt tcacggtgga
    10921 ggggtaaacg ggcagttgga gctgtcccgc cagggtgatg agctggtcga tggcagcggg
    10981 tcggtagatg tcggcgggga ccaggcaggg acgcctcttt tcctggacca gtcgccgggc
    11041 cagtttcgcc gcggtggtgg tcttgcccga gccctgcagg cccaccagca tcactccgat
    11101 ggggggcttt ccgatgaagt gtaagggttc ggcgctgccg cccatgaggt ccttcaagga
    11161 ctcatgcacg atcttgatga cctgctgccc cggggtcagg ctttgcatca cctcctgccc
    11221 cacggcccgc tcggcgatcc ccgcaacgaa atccttggcg actttgtagt ggacatccgc
    11281 ttccagcagc gccagtcgca cctcgcgcag ggagtcgcgg atgttttgct ccgtcaactt
    11341 gccctgcccc cgaaggtgct tgaagatctt ctgaaacttg tcggacagat tctcgaacat
    11401 cgtttccacc gttttggttt ggacccctga agcatgcagc gtctcctatg tatccacctc
    11461 ggggagttct gtcaagaaat cggaatcctt ttccattttt atcggcgggg accggcggtt
    11521 tcgcacggtc gggttggggc ggaaacggcc cgcccatttt cattttgcgc aatcccccgg
    11581 ctggggtata attccccacg aaatcttctc ttccttctcc cacgacatct cgcttcttcg
    11641 gcaccgtctc cgcagaatcg aaggttcgcc ccgccgggtc cgcgaacggg ggcgacgaac
    11701 gagtggaaag ccggacccca cgaagacaac cgccaaccta ccgataggac catgaactga
    11761 tggcacaacc gtgcgatgaa gggccccgtg cggagaaccg gtcttcggga gatttccggg
    11821 gacgtccgga cgaggaggtt cttcagaccc tgttcgaaaa cctcccgaac cccgtgatga
    11881 ttacggactt cgaggatcga atcgtctatc tgaaccctgc cgccgtcgtc cataccggct
    11941 gttccgatta caggacaacc ccccgtttcg gtgcggattt tcttcgtttg gaaaaggacc
    12001 cccatgggag ccggccttcg agtgaatccc tcgacgggct ccccccggct gatgccccgg
    12061 gatatctcaa aggaaaggat ggttcctggc atcccgccac cgccggcggt ttccttttgg
    12121 gaggggccca cgggcagtcc ccgggtcgtt tgaccgttct gaagatccgg cacccggaac
    12181 ccacttccga tcccgacaca ggccccgggc tccacgctcc cttctggaac ccggaaagca
    12241 gctttcccgt gagggacccc cacgcgatgc tcaagagcat cctggactac tttcccatgc
    12301 ccttcttcac cgtggatcgg cacctggtgg tcaccagcat gaaccagcat ctcgaagagc
    12361 tctccggttt tcgccgcgag gaagtccttt accggatgac ctgtgcacaa ctgctggcca
    12421 cccccgagtg taacaccgaa tcctgcgtcc tgcgtcagac catggaaaac cggtcgcccc
    12481 tggtgggggt cgaacggtgg atgaaagacc gggaaggaag gaccattccc attcgggtct
    12541 tctgcgccat gatcaccgac cgggagggac gggtcatcgg cgggtttgcg gcccttaggg
    12601 acatcacctt catggtggag gcggagcaaa ggatccgcct tctcatcgag atgagccagg
    12661 aagggattct catggcggac gaaagccgtc gaatcctgta cgtcaactcg aagatggccg
    12721 aaatggcggg agaggaaagc gaccggctgg tgggcactcg cctggacaaa cttctctccg
    12781 acgagttcat ggaactgatg gaccgtcagc tcgcccaaac ggaggacggc ggccgacagc
    12841 acctgtgttt ttgctccacc tttcaaccct tgaagacccc cagcctggat tacctggccc
    12901 tcgagacctg catcgcggtc tcccgggtgg gcagaacccg ggtcacctgc ctgtactttc
    12961 gcgacctgac caagcacatc gaattcgagc gccagctccg aagcgccaac gactttctca
    13021 acagcatcat acgaagctcc gtggacggca tcgtcgtttt ggacaacaag ggcaaggtgg
    13081 tcatcttcaa cgaaggagcc gagcggatcc tgggctacaa ggccgaagaa gtcatgggca
    13141 gccccgaact gttccataga atctacagct ccgagcaggc tcgagaaaac atgcgccgca
    13201 tgagaagcaa cgagtacggc cctccgggaa agctcagcgc cacccgggtg tccctggtgc
    13261 gcaaagacgg ggagatcgtt cccgcaaact tctctgcggc catcatcaag gttggagacc
    13321 gggaagtggg cacggtgggg attttctcgg acctgcggga agtcctgagg atgcgccgtg
    13381 agctcgaaga ggcccggact cagctgatcc aggcggagaa gatcctttct ctgggaaggc
    13441 ttgccgcggg ggtcgctcac gaaatcaaca atcccctggc aggaatcctc atctacgccg
    13501 acatgcttct cgaggatttt ccacccgact ccccctggcg cccggacctg gaggagatcg
    13561 tgcgccagac gctccgttgc aaggagatcg tcatgcggct cctggagttc agccgacagc
    13621 ctttgggcaa gcgacttccc tatgacatga acgaggtcat cggccgatgc gtggagctgc
    13681 tgcgcaacca gtccatgttc ctgaacattg acttctgcct ggacctcgag aaggacatcc
    13741 cccccgtcca cggggacccg ggccagattc agcaggtttt caccaacctc atcctcaatg
    13801 ccgccgatgc catgggaggg cgcggccgct gcaccattac cagcggctac agcccctcta
    13861 cccggaccgt cttgctgaaa ttcatggata cgggacccgg catccccaag gaaaacctgg
    13921 ataagatctt tgaacccttt ttcaccacca aacccccggg ccagggaacc ggcctgggat
    13981 tgtccgtagc gtacggaatc gttcagcaac acggcggaaa catcgaagtg gggaatctcc
    14041 ccgacggcgg agccgtcttt tccatacacc tgcctctgga accaccggaa taccagagtg
    14101 aaatcctgga aggctgaggg tctatgagca ccgtactggt ggtcgatgac gaaaaggtca
    14161 ttcgagaagg ctgcagccgg gccttgagcc gggcgggctt ccatgtgctg ctggcgtgca
    14221 acggcaagga agctttggac gttttggctt cgacccccgt ggacgtcatt ctttgtgatt
    14281 tgaaaatgcc ggtaatgagc gccgtggagc tcctgttgca ggtatccgcc gatcatccgt
    14341 ccctgccctt catcatcatt acgggccacg gcaccatttc cgacgcggtg gaatgcatgc
    14401 gcaagggcgc ctacgacttt gtcaccaagc cgtttcgagt ggaggtgctc ctccgcacgg
    14461 tggactgcgc cgcccggggg ctcccgccac ctcccaaggt ctaagagacg gcacccggca
    14521 caccaggacc caggacttca tgggcaccgt tccgcttggt tggttgggtt ggtccggtca
    14581 ctcggccggt ttggccccga aagacagggc ttcatgggta ccattctggt tgtagacgat
    14641 gagaaggtca tccgggacgg ctgtagccgc ctgctttcct cggaagggca ccgggtcctc
    14701 acggcttcgg acggaaagga agccctggag atcctcgaat ccgaggcggt ccacgtcctc
    14761 ttgtgcgacc tgaaaatgcc cgtcatggac gggatcgagg tccaaaagcg ggtccgggtc
    14821 gaatatcccg agctgccctt cgtggtcatt acgggacacg gcaccatcga taccgccgtg
    14881 gaatgcatga agaacggcgc ctatgatttc atcaccaagc cgtttcgcac ggaccacctc
    14941 cagttcattg tccgaagggc attggaaaag agggagctgg aagagaagac ccgggtcctc
    15001 cgggaggccc acgagagaaa cctctacgac ctggccgcag agcagagtcg agtccgcacc
    15061 atcgtgcact gcatggccga aggcgtactg gtcaccaatc gagatctcga agtggtgctt
    15121 cagaacccgg ccctggcccg cctgctggac cttccgaaag tcccggagca gccgaccctc
    15181 ctgtcccggt acctggagga acctttcctt gtggaagcca tcgagggcct catggagagg
    15241 gcccgggacc ggtgggaaat ggtttcccgg gagttccgca aaggttccct cgacctgcgg
    15301 gcgctttcgg ctcccatctt cggcggggcc ggagaagttc tcgggagcgt gaccgtcttt
    15361 caggatatca cgagctttaa ggaactggac cgcatgaagt cccatttcgt gcagatggtg
    15421 tcccacgagc tccggtctcc tctggccacg gtcctgcaac agatgggagt catcctcgag
    15481 gggctggccg gggaactcaa cgagaagcag cggtccctcc tggacaggtc ccgggacaaa
    15541 attcaaagcc tgttggacct catcaacgat ttgctcgatg tggccaagat tgaaagcggt
    15601 catgccgttc aggagcaggt ccccctgaac ctgggagagg tgctgagcga agtcgcggca
    15661 ttttttcgag aaagggccca aagacaggcg attgccgtcg agttggaaat ccaccctcac
    15721 ctgcccttga tccacgccga ccgacgttgc atggaagagg tcttcaccaa tctcgtcagc
    15781 aacgccattc aatattcccc cgacggaggg cgcgtgcgtc tctgcgcaat gccctgcggg
    15841 gaaaacgtcg aggtgcgggt aacggacacc gggatcggga ttccctccga agaaatcccg
    15901 aagattttcg ataagttcta ccgggtgaaa caccccagga cccggcagat catcgggacg
    15961 ggcctgggac tggccattgt caagggaatc gtggaatccc atcgaggttc cgtcgaagtg
    16021 gaaagcgagt tgggagcggg cacgaccttc cgtttgcttt ttcccgtaat ggaaacaagg
    16081 ggatagctgc ttaccatggc agaacttaag aatcggcatg ccgtcctgat tgtagacgat
    16141 gaatcggccg tccggaacgg catcgcccga gtcatcgaaa agaaaggcta tgatgtccgg
    16201 acggccgaca gcggcgaatc cgctcttgag ctggcggccc gggacccctt tgacgtggtc
    16261 ttcctggaca tcagactgcc cggtatggac gggatggagg tcttgaggca actgcgggca
    16321 aagaaccccg agaccgcggt catcatgatc accgggtatc ccaccatcga tacggccatc
    16381 gagtcgctga ggctgggcgc cctcgactac ctggtgaaac ctttccgggt ggacgacctg
    16441 gaagtgatgc tcgaaaaggc cctggaatcg gcggtctccg acggcaagga accggacccg
    16501 gagctcgacg tcgggcgaga catcccgtca ctcatcggca agagccccgc catgcgcgat
    16561 gtctttctta agatcaagcg ggccgctccc agcgacagca ccgtcctgat tacgggggaa
    16621 agcggcaccg gaaaagaact ggtggcccgg gccattcaca acctcagcct ccgagccgac
    16681 aaggaatttg tggccgtgga ttgttcggcc ctggtcgaga ccctgctcga aagcgagctt
    16741 ttcggccacg tgaagggttc cttcaccggg gcctttcaga ccaagcacgg gttgtttgag
    16801 ctggccaacc ggggaacgtt cttcttcgac gaaatttcga atctgagccc gaacatccag
    16861 gcaaagctcc tgcgcgtcat ccaggaacgg gaattcatga aggtcggaag tcagaaaagg
    16921 atcaaactgg acattcggat catcgcctct tccaaccgtg acctgaggga agccaccgcc
    16981 gaggggacct tccgggaaga ccttttctac cgcctgaggg tcgtgcccat tcgcctaccc
    17041 cccctcaggg agcgcgtgga ggacattccc ctcctggcgg agcacttcat cgaaaagtac
    17101 agccggaaag gaaaccgagc cgtccaggga atttcccggg aagccatgga cctgctgtgc
    17161 tcctactcgt ggcccggcaa cgtccgggaa ctcgagcact gtatcgagcg gatcctgatc
    17221 ctggaggacg gagaaattat ccgcccggag aacctgccgg ccttcatcac ccaacgacag
    17281 ggggagtttc ggatcttttc cgaggaggtt ttctccctaa aggagttgga aaagcgctac
    17341 attcaattta ttcttcgccg gacgaaagga aggaaacagg aaaccgccca aatcctggga
    17401 atcgatcgaa agaccctgag cgtcaagatc aagaaatacg ggatcaagac gggttagggg
    17461 acctttggaa aggagcctcg catgaacagc caccctattc tcagtctcag cgtcctggga
    17521 gttctcttcc ttctcgtgcc gtgtttttcc ccggtgcagg ccatggggaa ggataccatc
    17581 gccaccggat cgggacccgt ggaagtgggc ttcatcggcc acggctctct cgtattcttc
    17641 tttcaaggaa aaaccgtcta catcgatccc gtagccgcct acggggacta ttcggcattg
    17701 cccaaggccg atctcatcct tctcacccat gaacacaagg accacctgga cccccagacc
    17761 ctgtccctca tccggaagaa agactccgtc ctcatcgtca cccgggcgtg cgccgaccgg
    17821 ttgaaagagg ggacagtcct ggaaaacggg gatgtgaagg aggtcctgga gatttccatc
    17881 cgggcggtcc cggcctacaa ccttgtccac atgcgaagcg aggggcaacc ttatcatccc
    17941 cgggggcggg gaaacggata cgtcctgacc tttggagaca agaagattta cgtggccgga
    18001 gacacggaga atgtgcccga gatgaaggcc ctccgggaca ttgacgtcgc ctttctgcct
    18061 atgaatctgc cctataccat gaccccggaa atggtggcgg acgccgccgc ggcatttcga
    18121 ccgaaaatcc tttatcctta tcactacgga gataccgatc cccagaggct ccgggatctc
    18181 ctcaaggatg aaaaggacat cgaggttcgc gtccgtaccc tccggtagcc cgcgggggga
    18241 gccccccggt tttgcctcgt ccgtccacgc ccaaagggac cacccaagga tgaccctgga
    18301 agacctctac gagctcattc ccgacttccc atgcctcccc ggctgcacgg aatgctgccg
    18361 ggtttccgct cctccttccc ggacccccgg ggaacaggtg aggctgatcg agtacgttca
    18421 aagtcatgcc gtccgcctcg atccccggga agaaggctgc ccctatcgct ccgacgacgg
    18481 atgtctcgtc cacccggtgc gccccttcat ctgccgcctg tacggcacat cgcccggcta
    18541 cctctgcagg atgggaatcc ggcccgttag acttttgcac gaggacgaag aagccgacct
    18601 gttccatttc tactacaccc atttcgcccc atgaatcatc ccatccacca ttcctgttcc
    18661 atcgaggagg caagagacga tgaagaccca ctccattgcc gagatcttcc cgccgagccc
    18721 cggcaaggtc cggctgcaaa ccctcacgag aaatctcacg gcggggctcc tgagttgcct
    18781 ggttttgtgg agctgcaccg ccgcaagttc gcaaccttcc cgtccctccc cctccccgcc
    18841 gccgacctca ttttcccatc cgccccaaga acggaccccg gttcagtctc ccgtcaccga
    18901 ccggggatcg acctaccggg tggtgaccga ggaggagctc atccaatggg agcaaaccga
    18961 tccgggtctc agcttcaacc ggtgccggga aatcctttca cggttgaaca tcaaggccgg
    19021 gtatcacatc tccgacgacc tgcgccgggg gcgggcgctc ctggccccca acgacttcag
    19081 ggctttcaag gactggaccc cgctgccctg ccacgcaccg aaactggcgg agttccccaa
    19141 ggcgatcctg gtggtgaagg agattccctt cctgggatgg tacgagaatg gattcctcac
    19201 gggggatgcc cccgcctgca taggcccccc cggagaagat acccgggccg gacgattcgt
    19261 ggtgatcgaa aaggacgcgg accataagtc ccggagctac cgcaacgatc tgggcgagcc
    19321 cgcatggatg ccctgggcct tgagggtgta cgacaccgtg tggattcacg caggggacgt
    19381 ttccggcaaa cactgctccc acggatgcat catcctcccc gtgaagacgg ctgagaggct
    19441 tttccactgg gcggatcttg aaaccccggt ggtcatcgtt gagagccttt cggacctgta
    19501 gggacgacac ctgggccggt ccggcctcgg cggccaaatt gcagcctgta ggtagccggc
    19561 aaaaaggagg gcaatgaacc gaaagacgag cgccccgagg atcccgtagg ccagggcctt
    19621 cttttgccgg tgccggggca gggcacgcac caggatggcc ggcaccaggg cattttccac
    19681 cgacagtatc ccctcgagga cagccagggt gagaatggcg atcccgtcca ctataggaag
    19741 ggaatgaatc tcacggacga tttgggcaaa gattccggaa cccatgcaac cctctccggg
    19801 aatgctccca cgtggttttt tgtgacgccc tcaagccttc ttcaggaggc ggtccgggct
    19861 ttcgacggga gacggtctcg caaacccgca acccgcctcc agggtcgctc cggatgggaa
    19921 cccaaacccc gcggttccga aatcgtccca aggtccgcct tgtgtcgacc ccctttccgc
    19981 ttcgggcttc cttgccgtcc ggaacccata cccacggccc caagcagctt gttccattcg
    20041 atccaccctt cgggttttcc agatgctaag ggagaacccg ggaggagaca aagcgccggg
    20101 ggggaatttc agactcgctc ccgggtcgta agggttgaac cgagggacga ccggatccga
    20161 accctatctt ggacactcat ttccgtggcg cccgctcaga aaaaggttgc atcacctggg
    20221 cgaatcttgg aataagtttt cgtgtccggt tcccggacga aggaaagaaa ggaagccttt
    20281 ggggatgact gggacaagtc gggcaggctg tgcggaacgc gcaacggaag gcagcctcct
    20341 ggggaaacgg atccgggagc gtatcgccga cttcagcgcc gtttacatgc acgtgaccga
    20401 gttgcggcaa tggcttcgcg acggccgccg aagggtcggc tccgagatgg ttgaaatcct
    20461 ggtgagcgtt ctcgatgagc cgacacaccg atctcaaaag caggtctttt tccttcacag
    20521 agaggctgcg ggggtgctgg cggaactcat cggggacccc ggtctcccgg tcgaggtccg
    20581 cgcccttgca ctctcccgac tcaaaaggct tgccgcctcc tccgtgcaga gcgttcaccg
    20641 cgccgtgtgc gaagccctgg ggtccctgcc cctggacatc cggggtccgt tgatctcgcc
    20701 caacggagac ggagaagtct cccggttggg gggatgggaa ctctcgagag ccctcgcctc
    20761 ccaggggtgc gggcctccct atcgggtggg aaggagcttc gtttcttcgg gtacggattc
    20821 ctcctttgtc gtcagcatca aggtctggca aggacccgac gcccataccg cagccgtccg
    20881 ggagtggacc tggatggagc tcctgaaccg ggtcaaacct tccatcccca agaggtttga
    20941 cattcccgag ccccttttgg ctgccacgac ccccgtcttc caactcacct ccttcccgga
    21001 cgcctgcggg aacgagacgc ctcttgctga aaaccgctgc attcttttcc gagcccctcg
    21061 ggactactac cgctatccca acggctctgc cgtgtcgcgt cttctcgatg agtccgagtt
    21121 cttggaggta atccttcgga atgcctggtt atttggaaag acggcctcca tggggatcgt
    21181 tcacacggca cccatcccct tgtttcacaa ccgaacccag gtggacagga gaaatgacgg
    21241 ggggatctac caatggcccc ggggaggaag gttggaccga tggctggaat cctgccgcca
    21301 tcccaacttc ggaatcagcg ggctgaggga cttcgaacac ctcaggagcc tgaggaccgc
    21361 aggggacggg cgtgggttct accaggacat cggaacccag attttgagcc ttgtgctggt
    21421 cgcgggaagc tactttcgcg gcaaagcccc ccaaagggtc gggctggatt cccggggaaa
    21481 acctgtggac gctcgagacc ttttccaccg gcctctcctc gaggacctcc tccacggcat
    21541 ctacagggag tatcatcgag gactggtggg agcgcccccc cgggaacccg tccctttgga
    21601 tgtttctcac cttgccgagc gaatgatcga ggaaatgggc gtggaccgcc acatggaaga
    21661 aatcttccgg gtggcggacc aagaaaggat gtcccaggcc gaattcgagg ggttcctggc
    21721 agaacggggc tgttcccggc aagaaatcgg tcggcggctc aagggggccg aggacatcgc
    21781 cttgcaaacc ggaccgcact tgggccgatt caacggcccc atctccctgc cggagatgct
    21841 tcgtttcgtt gccgtcacgg ccgccggctg cattgccgac cggttcatga ggtcaaatct
    21901 gtgcccttga ccacggggga tgacgggaag tcttgtgcga atcctctgca caggccgccc
    21961 gattcgggaa gtcggcccga aagagggatc caaattcccc ggagtgagct gttgaccctg
    22021 gaaaaacaag gaggtttcca tgacatcgca caaaggggtg cgttttcggt ctcactacct
    22081 cttccggtcg gtcctggtcg cgggggtcct gctctgcttc gccgccttca gcgccgaagt
    22141 gcaaggggat cgaaacggcc tctatcagct ctacctcaaa gccgtcgatg acgccaagac
    22201 cgtcaatccc cacaaaatct cgcgcaacct cgtggccatc accaagggaa accccaatct
    22261 ccccctccgc tgggaagggg ttcccgagaa atcccgggtc ctggtggtca cctggacgga
    22321 caaggattat tacgacgact acgttggacg ggattatgtg cttcccccga gcgcaaacgt
    22381 gtgggtaacg gcggtccccg aactcaagga gtttgtccgc aaacacgttg tcccggttcc
    22441 tcccctgcgg gtggagcagc tcctgggcct tccccctcat acggggaaga ccaagttcgt
    22501 cgaaatctgg gccgatcgaa agagcctctt tcggcccagc ccggacccgg agatcaccga
    22561 ccatgaagcc gaactgcgtt ttcccaccaa agtgaatcca ttcctgaaat acgatcccgg
    22621 tgcatccatg atcgagtgga actccgatct gcagaaggaa gtgaaataca cctatccgga
    22681 gtggttcaac catcgcaagc ggaccatcta caccggggat tctccctacc cctggacccg
    22741 gctcggttac acctacgact ggggaaatgt gcagacccgt gtggggttaa gcgaattcgt
    22801 catcctggga ggctccacgg tcggaattca cgccgttcgg tccaatgccg aatacctgaa
    22861 cccggcgctt ctcaactgag ccgggtcctt ccgttcctag attgagcgat ttggccggtc
    22921 ggaatcccgt ttcgtcattc ccgcgcaggc aggggtccgg aaagtgagtg catacccgtt
    22981 ggatgcccgc cttggcgggc atgacgcctg gatgctctcg gaatcgctca actcaggccg
    23041 ttgccttccg cggcggggga ggttttccct ccttggggaa ttcttcccca tttggatatt
    23101 tgtttaacga tatccaataa atagctggag agccccgggg gtccccgggg cgtttttccc
    23161 cggaagggac cacacgcccc ggcctcgatc cctcctcgtc cccccacaaa acggccgccc
    23221 gggcggctca ttctttcctg gcatgtggct tgccccaagg aattttccca tagccaaaga
    23281 ccagcaccct atgacgtttc caccgactgg ggatgagcga tggccgcaac tctgggaacc
    23341 cttttgatcg ctgaaccggg atgcaggctg gtgtcggctc tgaagccgtg gctgctccat
    23401 cagggataca aagtgaaagc cgtcgaggac atgaaaggcc tcttgaggac gctccaatgc
    23461 gaaaacgtca gggtgctggt cctggacgtc tgcctggccg aatccatgga ttatgaggca
    23521 atctccatca ttaaggggtt gtgccggaat ctgcccatca tcgtgacgac ggagagcaac
    23581 aatcccgaac aggaaagcag cattcgacag aaagggattt tctactatca cgtcaggtct
    23641 tttggagtgg aagagctggt actggccatt accaacgcca tgacgcggca tccttgagag
    23701 caggcgggcg gcgccgacgg ctcaaagggt atgaggaaac ggtcgagctt tcagacaacc
    23761 cgggaagatc tcatcaagta ggaatgcctt atgagccttg aagacaaact gacctccgga
    23821 aagtttctag tcctcgcgga aatggatccc cccaaagggg tggatgtgac cggtctggtc
    23881 gccaacgcct ttcgggtgaa gggaaaggtc gatgcgtacg tggtgccgga gatgagcaac
    23941 gccgtcatgc gcatgagctc cctgggaggc tgcatggtgc tccaatccaa agggctcgaa
    24001 accgtgctcc aggtctgctg tcgagatcgg aatcgtctgg ctctccaggc cgatctcctc
    24061 gcggcctatt ccctggggat ttccaccctc atggccgtgg cgggagtgga tcccggttac
    24121 ggggaccacc accgcaccct ggccgtgaac gacatcaacc tcatggaact gctccagggg
    24181 atccgcacac tccaggaagg aaaagacatg gcagggatcg agctcgccgg agcccccaag
    24241 ttttgtgtgg gttccaccac caacgcgggc accatcgaag gtcccctgga agcggaaatc
    24301 gaggaaatgg agaaaaaggc cgccgtaggg gttcgtttct ttgtgactcc tccggtcttc
    24361 gacatccagc ctttgcagcg gcttctccgg gcaacggcgg atcgcggaat gaaaatcgtt
    24421 cccacggttc tgctcctcaa gtcggccgga atggccggct acatcaatcg ccacatcgag
    24481 cacgtaagcg tgccgtcgga attgattcga aggattcaga aggctccgga caggccccgg
    24541 gaatgcgtga ggattgccgc ggagctggtt tccgccatca gaaggacccg ggccgccgga
    24601 gtgctgctct ccaccatcgg atgggaggac aagcttgccc agatcttgga aggggtagaa
    24661 acatgaagag ggaagaagaa gctcccgtcg gttcggtatt ggttgtaggg ggcggggttg
    24721 ccggaatcca ggcctccctg gacctggcca attccgggtt cttcgtgacc ctggtcgaaa
    24781 aatccccggc catcggcgga gtcatggccc agctggacaa gacctttccc accaacgact
    24841 gctccatgtg catcctgtcc cccaagctcg tggaatgcgg ccgacacccc aacattcgtc
    24901 tcctgactct ggcggacctg gtggacctgg acggcggggt ggggcgcttc acggccacgg
    24961 tacgccgacg tccgaggtac gtgaacctgg acaagtgcat cgcttgcggg gtgtgtgcgg
    25021 aaaagtgtcc gagaaaggtt cccgacgagt tcaacgaacg gttggggaac cgcaaggccg
    25081 ccttcgtcaa gtatccccag gcggttcctc tcaagtacgc cgtggaccgg gagcattgca
    25141 tctatttcga gaagggcaag tgccgtgcct gcgagaaatt ctgtccggcg ggagccgtcg
    25201 agcttcagca agaggagatc cgtgagaaga tcgaggtcgg gtcggtgatc ctggccccgg
    25261 gctttcaagc cttttccccc acccgctacg aatacggtta cggcaggcac ccggacgtgg
    25321 tgaccagcat cgaattcgaa cggattctca gcgcctccgg cccgttccag ggccatctcc
    25381 ggcgtccgtc ggacgggaag gtgccccgca agatcgcctg gctccagtgc gtggggtccc
    25441 gggacctcca acacggcgcc cacggctact gttccggggt ctgctgcatg tacgccgtca
    25501 aagaggccgt tgtggccaag gagcatgctt cggaccccct ggatgccgcc gttttcttca
    25561 tggacatgcg gacctacggc aaggacttcg agaagtactt cctacgggct cgggacgagt
    25621 acggcgttcg ctttgtccgt tcccgggtcc acaccgtgga cccgtccccc acgggggatt
    25681 gcctgcttat cgggtacgtc gacgaaggag gtgccgtctt cgaagaagag ttcgatatgg
    25741 tggtgacttc cgtgggcctg gaaatcggaa aagataccga ggatctggcc cggcggatcg
    25801 gagtcgagct ggacgagacc cgttttgtgt ttcctcgaga gttcgccccg gtggccacct
    25861 cgcgcaaagg agtctacgcc tgcggggcgt tccaggggcc caaggatatt ccggcctcgg
    25921 tcatggaagc ctcggcggcc gcctgcgcag cggccatgga cctggcggcc tcccggggaa
    25981 gccgggtgga agtcccaccg gcggttccgg aagaagacct ggctggcgag gagccccgca
    26041 tcggggtttt cgtctgcaac tgcgggatca acattggctc ggtggtggac gtgaacgaag
    26101 tgaccgccta tgccgccggc ttgccccacg tggcggcgac ctgtcagaac ctctttacct
    26161 gttcccagga tacccaggag cgaatcaagg aggtcatccg ggacaagaag ctcaaccgca
    26221 tggtagtggc ggcctgttct ccccggaccc acgaacccct cttccaggag actcttcgag
    26281 cctgcggcgt caacaagtac ctgttcgaaa tggccaacat ccgggaccag gattcctggg
    26341 tgcaccccgc ggatccccgg aaggccacgg acaaagccaa ggacctggtg cgcatggccg
    26401 tagcccgggc ctccctcctc cagcccctcg aggaacgccc gctctccatc aagaggcgcg
    26461 gtctggtcat cggggggggc gtcgcgggcc tcaatgccgc cctttgcctg gctcggcagg
    26521 gattcgaggt ggttctcatc gagagggaga aaaccctggg ggggatggcc cgccagatcg
    26581 cccagaccat tgaaggctgg gatgtccagg aatacctgga ccacctggtg gccgaagtcc
    26641 aacaccacga gaagatccag gtgctcaccg aaaccctggt ggtcggattc ggcggctata
    26701 agggaaactt caccacggag gtcctggtgg gtcccgggcc ttatgaacgc aagatcgagc
    26761 acggggtcac catcgtggcc acgggagccc tcgagtaccg gccccgggaa ttcctttacg
    26821 gcgatcatcc ccgggtgatg acccaactcg agttgggaag gcagctccgc aacaatcccc
    26881 tggagatcgg ccggtgggaa aacctggtga tgatccagtg cgtgggttcc cgaaacgagg
    26941 agcaccccaa ctgcagccgg gtgtgctgcc agagcgccgt gaaacatgct ctccatctcg
    27001 ccgatctgaa tccggacatg cagatcacca tcctgtaccg ggacgtgcgg acttacggcc
    27061 ttctcgagga atactacaag tccgcccggg gaaaagggat tcttttcgtt cgtttcgacg
    27121 ccgaccgtcc gccccgggtg acgggggacg gcgagggctt gtgcgtgacc ttccgggacc
    27181 acgtcctgga ccgtcccatc cggatgaaga cggacgccgt ggtcctgagc gcagggatga
    27241 tcgctccgga caccgaagaa ctggcctcct ttctcaaggt ccctcgaaac gccgacggct
    27301 atttcatcga ggcccacgcc aagctgcgcc ccgtggactt cgcctccgaa ggcatctacc
    27361 tgtgcggaac ggcccacggt cccaaactca tccaggaagc catcgcccag gccatggcgg
    27421 cggcggcccg ggccgcctcc ttcctggctt cctcccatat gacgtacggg ggcgtcgtgg
    27481 cccgagtcca accggagctg tgcgccgcat gcctggtttg cgtgcgaagg tgtcccttcg
    27541 gaatcccccg aatcaacgaa cacggcgttt cggaaatcaa cgaagccctg tgtcaaggct
    27601 gcggcgtatg cgtgtccgag tgccccgcca aggccattca cctgtcccat tacgaagacg
    27661 accagatcat ggtcaaggta gatgccctgt ttgagacccc ggagcgtgac tatgaaagcg
    27721 tttgaaccgg tcatcgtggc tttctgctgc aactactgag cgtattcggc ggcggacctg
    27781 gcagggtcca tgcgactcca ttacccgacg aacctcagga tcgtcaaagt tccctgcacg
    27841 ggaagagtgg cggccattca cctcatgaaa tccctggaaa agggagccga cggagtcctg
    27901 gtggcgggct gcctcgaggg cgactgccat ttccagtcgg ggaacctgcg ggcaaggaag
    27961 cgggtcgatt acgttcgcag gattctcgaa accattggga tcgagcccga gcgggttgcc
    28021 atgtacaact tgtccgcggg catgggaccc cgctttgccg agattgccct cgagatgacc
    28081 gaaaaggtga gacagttggg acccaacccc gtgaaagcgg ccaagggtgg aaaggagtct
    28141 gcggcatgat catggccaag ccaaaacccc tgcaggaaat catcggctac gtgcgcaccc
    28201 tccacaagct cctggtggtc ggctgcaacg gttgcgtggc cgtctgcgaa gccgggggcc
    28261 tcaaagaggt ggaagtgctc tcctccgccc tccgcatgca cttcatgaag gaaggaaagg
    28321 tcctggagat cctcgagatg agcctcaccc gccaatgtga caaggaatac ctggaagagc
    28381 tcaaggaaag gatcgacggc tacgacgccg tcctctccct ggcctgcggt gccggggtgc
    28441 agctcctggc ggagatgtat gagaacaagc ccgtcttccc ggcagtggat acctgcttca
    28501 tcggggtcac cgaagaacag gggatgtgga gtgagcgctg ccaggcctgc gggcaatgcg
    28561 ttctttcgga aactgcgggg atctgcccca tcacccggtg ctccaaacga atgctcaacg
    28621 gcccctgcgg cggatccgaa aacggaaaat gcgaaatcag cattctcgtg ggcagggacg
    28681 tcgcctgcgg ctggcatctc attttcgagc gcctcaagaa atcgggtcgc ctggattcct
    28741 ttcgacggcc cgccccggac aaggactgga ccctcagtcg tgacggcggg ccccgccgga
    28801 tcatcaggga ggacgtcaga ccatgacctc gacaacgccc agcaaactcg aaaaaatcct
    28861 ggctgcaggc cacctggccg tcacgtccga atgcggcccg ccccggaacg gccgagggga
    28921 cgtggtggag aaaaagggag agcttttgcg cgggtttgtg gacgccatca acgtcacgga
    28981 caatcaaacc gccgtggtga ggcagtgcag tctggcttcc tgcatccgtt tgaagctcat
    29041 ggggctcgaa cccgtgctcc agatggtgac ccgggaccgc aaccgcattg ccattcaaag
    29101 cgatttgctg ggggccgcct ccttcggcat ccacaacgtc ctgtgcctca ccggcgatca
    29161 tcagcgtttc ggcgatcatc ccaactccgt caacgtcttc gacatggatt ccactcgcct
    29221 catccagacg gtcaagacca tgcgggatga cgggaagctc ctcagcgggc accccatgga
    29281 ggaaggcagg ccgcagatgt tcatcggggc cgccgaaaat ccctttgcag accccttcga
    29341 ggttcgggtc ctgcgactgg ccaagaaggt ggcggctggg gcccagttca tccagaccca
    29401 atgcatctac aacatggaga aattcaagct ctggatgaaa atggttcggg acgaagggct
    29461 gcacgaacaa gtggccatcc tggccggcct cacccccatg aagaacgtgg gaatggcccg
    29521 ttacatgaag aaccgggtgc cgggcatcga tgttcccgac cccattgtca aaagacttgc
    29581 gggtgtaccc aaggaaaacc aggccgagga aggaattcgc atctgcctcg agcagatcga
    29641 ggaactgaga ggggaggaag ggatccgtgg atttcacatc atggccatcg aatgggagga
    29701 aaaggtgcct gagattgtga agcgggccgg actgtacccc cggcctgcgg cggacgaacc
    29761 cggtggattg cccggcggca cgacggggag ctgaaagcgg ccccggtcgc accccggcat
    29821 cgaggaaccg acccaaagga cccccaaggc gcgagaaaac cccgccatcc ttctttttct
    29881 caggcctcga gcacggcaag acgccggatg ataagggggg agtccggacc gaccgagagg
    29941 gggtacttcg ttgacgcggt gaaccatgga tgcagccaac cttttgtgaa ctacggacag
    30001 gaggtatttc gatgagcaaa aaatacgtgt tggttgtgga cgacgatccc gacttcgtgg
    30061 aaacggtggg catgatgctc gaaagcaagg gctacgaggt tgggaaggcg tatgacggca
    30121 tcgagggcga acaatccatc ctggccagaa aaccggacct gctggttctg gacgtgatga
    30181 tgcccagaaa gaacggctat accctttgtg cggagctcaa gaagagccgg gatacccggg
    30241 acattcccat cgtcatgctc acggcggtgg gggaggcggt ccccacgact tcgtacagtc
    30301 atgccgacgg gatggccatg gaagccgacg actacatccc caagcccgtg gacacccagg
    30361 ccctgatcaa cgccatcgag aggctcttct cctgacaagg gagctccggg gccgggccgc
    30421 tgaggcgagg cccggtccgg aaatgcttat ctccctctgg gcccccggga agaggcgccc
    30481 tccccggatc gggtcgggcg cacggaggag cccgcggatc gtcgggacaa acggttccct
    30541 ctcggtggcc ccgggggggg agggagctca tggatcccgg ggggacatcc cggatcgaag
    30601 gcacccgcag gagacctccc ctgcaggact cccgcggggc tgcgttcgaa agatgcggct
    30661 ttccgcccgg gattcgacga agtaacaaca gcaccgatgg gattctcgag gattcggtgc
    30721 tatccctggt cggtgtactc tgtcccgtcc ggagcaggaa gggaagtcgg cctcgagggg
    30781 tgaaagggtc cgccccgggt cgcttctttc gcaccggtcg tttcaggaac ggcccacagg
    30841 cggcggccca tgaccaaggt tcggctggga ggaatcaagg tccttgccgg gcgctcccat
    30901 ctgaccaatc tttgcccggc ggatgagagc gccttgcacg atgtttgccg ccgaatggcc
    30961 gccgacggga tcaacctgtc ttttctcacc cacctttgca gcgatcacga ggggaactgc
    31021 tataccgccg cgtgcacgga gatcgcagac gcatactccg gatactttca ctggaaagcg
    31081 ggtggcggat catgccacgt gggaaatccg gaaccggacg tcaacatcct ttccattttt
    31141 cctcacaatc aaaatccctt gattgcgggc accctcatcc agctcatcgc ccgacagcga
    31201 acgcagccgc gagggttcgc cagttctccc tccgccattt ccgttctcat ccggggagcg
    31261 gacacatcca aggccatcga cgggcttttc gatctcttcg aatttccgac ccaccgttcc
    31321 cccttggact ggcacgcagc ctacgaaggc caggaacacg ttctcaagga gatcatctgt
    31381 tccttccagg aagacgtgat caaggtgtac gacttcgccc gtcaagcggg cttcgaactc
    31441 tggaatttca ccgtgtccgg ggatgggttg gagcagctgg ggaagtttct cacggccctg
    31501 ggaaagaacc aggcccgcag cccctttctg gtgggccacc cggatactcg aaaccgcacc
    31561 cacttttcca tctgcctgcc ggaaccatcg aggcccttca tcgaagaatc cctttcggca
    31621 caaccgtttg cagaggcggc gtcctgcgcg ggatccgttg cggtggtctc tcttcacggg
    31681 cctcacttcg gagaccgctt cggaattgtc catgccctgc tgcgatccct cgaacagggc
    31741 cgcattctgc ccctggctct ctgctgcgtg gtctccacca tctccctggt ggtggccgcc
    31801 gacgagctcc cggacacgct gcgggcgatc gagacctcct tccaaatccc ccacagcggc
    31861 tgagattcct cagcccgcga aggaaccctt ccgcacatgt tgaagcgcgg cgaagtcctt
    31921 cgttgatgcc ttcatttgcc gacccgtcgc atcctgatac cccctaccgc aagccctcaa
    31981 tccctcaaag atttccatcg cttcgaagtg gaacgctaac gatgaactaa gcggcggcgc
    32041 gaaggtgctg ggcgtccacc gagccaccgg ttgcgccgtc cgcttgagtg acaggttggc
    32101 ccttcttcgt catccgaaga acttctcgta ctcgtcgtga ccgccgaccc agaaccacgt
    32161 gacagtgtcg ccctccataa tgcccaaagc gcgaacgcct cgcgtgagcc tcacagacca
    32221 aacgttctcc tcttggttga tgcacttgaa ccgaagcgag gggtggaaag ggtccttgga
    32281 ccaaagacga tatgccttct tcgccttggc tctgagattc tggtccagcc tgccatacaa
    32341 ttcccagaag gacggcagcg tcgcagagtt cacagccttt tccgatccat ggggctggcc
    32401 ttgccctctg cgatttcttt ccgagcctgc cgcgcagcag ctacgagctt ggtctgggtt
    32461 cgggtaaacg actcgtccca tttcgcctcg tcgcggatgt cctcgatgta ctcgcgcatg
    32521 tgctcgagca cccgctcttg gatctgctcc ggaagagact caagcatctt catcatcgtc
    32581 gccatagttg cagttgaggc catttctcca gtctccttga tcctatgcat tgtctatttc
    32641 tacgttgggc caacg
//
LOCUS       DAOOVC010000030        31898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_942_length_31898_cov_6.822630, whole genome shotgun sequence.
ACCESSION   DAOOVC010000030 DAOOVC010000000
VERSION     DAOOVC010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 31898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31898
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_942_length_31898_cov_6.822630"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLM79_06115"
     CDS             <1..548
                     /locus_tag="PLM79_06115"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOV85918.1"
                     /translation="DDLPDGGAQVFAATIRAVRDRVPGCTVEVLVPDFSGDSEALDVV
                     LDAHPEVLGHNLETVLRLYPRVRPRAQYRRSLDLLEAAKKKRPDLLTKSGIMLGLGEI
                     PREIFRVMEDLRGVHCDILTLGQYLRPSIRKLPVERYYPPREFEALRKAGMDAGFRWV
                     EAGPLVRSSYHAATPVQALRT"
     gene            complement(550..894)
                     /locus_tag="PLM79_06120"
     CDS             complement(550..894)
                     /locus_tag="PLM79_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85919.1"
                     /translation="MVKHFGIQENWEFNGLLTLRWLKFPWDRYLDTSFAAGDGLSYAT
                     EVPVIERQRHGEESARFLNYLMFEFTFGLPKYPRWSLITRLHHRSGVFGLFDGVHGGS
                     NYLCFGLRYDFL"
     gene            complement(918..1163)
                     /locus_tag="PLM79_06125"
     CDS             complement(918..1163)
                     /locus_tag="PLM79_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85920.1"
                     /translation="MNETGTFKKGVGMQSRLAVMLIALMMFFAQQVHAQNNWFMTVYG
                     GQNTGGSIDDFITFDNDFRDSYLFAVTGERTSIPGAT"
     gene            complement(1290..2696)
                     /locus_tag="PLM79_06130"
     CDS             complement(1290..2696)
                     /locus_tag="PLM79_06130"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOV85921.1"
                     /translation="MSAEQVQEERIRKLNDRPAVPGDYVLYWMQQSQRADYNHALEHA
                     VRRAEELNRPLVACFGITDRYPEANLRHYAFLLEGLAETHRLLARRSVKMVIRRGPPE
                     EVARELGRRAAFLVCDCGYLRHQKAWRAHVAREVPCGVEQVESDVVVPVEAASDKREY
                     AARTLRPKIHRHLSKYLVEPEQAIPYKSSLALPIAGLEAEQLEDWASWRNFPADPSLK
                     TVSPFFRGGATAARETFEEFLRGRFSRYPANRNQPQTDDVSHMSPYLHFGQISPLELA
                     LRVTRLPPSLQQGKDAYLDELIVRRELAANFVHFTPDYDAFSCLPDWARRTLDAHSKD
                     PRNPLYGPEQLERAQTHDPYWNAAMVEMKVTGYMHNYMRMYWGKKILEWSPSPEMAYR
                     TLLALNNRYFIDGRDPNSYANAGWIFGLHDRPWGGRSVYGTVRSMSAAGLERKCDIDA
                     YVRKVERLAREAGESECG"
     gene            complement(2842..3945)
                     /locus_tag="PLM79_06135"
     CDS             complement(2842..3945)
                     /locus_tag="PLM79_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV85922.1"
                     /translation="MNDLSKVYEALAAHLDRLPEGYPRTPTGVELRILRRLFTPEEAA
                     LAVMTTLRPEPPEAIAVRAGLDPREAARRLYEMSRKGLIYRLRKGEQVYYMAAQFIIG
                     IWEFHVNDLDPELIRDVNEYIPYFFRQDSQQKSPQLRTIPIDRAIESREEILPYEEAR
                     KILAGQEKILIAPCICRKEHQILGKGCGRPLESCFVFGVGAQYYEENGLGRVIDHREA
                     FQILDRAEEAGLVLQPSNSQQVVNICTCCGCCCQILKNLKALPAPARVAATNFFAVVD
                     GECCTGCETCVSRCQMEAVRMDGATAVVLRERCIGCGLCVPTCPEGALRLEQKSENER
                     FTPPQNRMERFKKITAERMAKAKRVSSPEQGTP"
     gene            4153..7197
                     /locus_tag="PLM79_06140"
     CDS             4153..7197
                     /locus_tag="PLM79_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV85923.1"
                     /translation="MNGELRPDKEFKDDILREQIRLSMKQLPTMQAASFVVAIVLAYS
                     VRHIVPASNILAWLLMILAVVASRTILYYRFVRVREQSFDGEYWKKLFLVLALISGVL
                     WGISAFIVFPADQQGLIFLFVLVMASLSAATTVSHSPIRLGPTAWAAPAMLLYAVRCF
                     ADGGELGYTLCALILLYLFTVLCYSFHQNRAVTSAISLRFENLGLLAELRNARELLEK
                     KVEERTAELKRTNETLSREVEERRSIEAALRLSEKKYRDLVELANSVILRWDPKGTIT
                     FANKFAQQFFGFGEEELVGKSILETIVPPRSPEGGDLEEVIREICRTPHGFETREREN
                     LRKNGERVWVSWTHRPIYSENGDLVELLGIGQDTTQRRLTAEALRVQEERLRLAMAAS
                     RQGWFDLNVRTGEVVASPEYARIMGYEPSEFSSNLRAWMEGIHPDDRDKVLDAFNECI
                     ETGETRSMEYRHRTKTGQWKWIRSTGKLVQYDSQEKPLRMIGTHADISERKQAEEALR
                     ESQERFRELTELLPETIFEADVQGTLTFVNRSGMDHFGYSQEDFEGGLNCFDMIAPED
                     RPRTLENAARLLSGGSIGLNEYKALRKDGTVFPAIIRSSAKVRDGKAVGFRGIVIDIT
                     ETKRLEEQLRQAHKMEAVGTLAGGIAHDFNNLLQTILGFSELLLLEKTEEEEGYPELQ
                     EIFRAAERGAELIRQLLAFSSKIESKLQPVNLNQVVENVRVLLERTIPKMIRIELRLA
                     EDLQYVNADAPQMEQVLVNLGVNARDAMPQGGTLTIETENVVVDKEHHRAHPELLPGH
                     YVLLTATDSGQGMDGTIREHIFEPFFTTKEVGKGTGLGLAMVYGIIKNHSGHIFCTSN
                     PGQGTRFSIYLPATQPVEALPYRMEEGEEPRGGNETILLVDDEDSIRDFARRILETHG
                     YRVIPAPSGENALGVYQKDGSGIDLVILDLIMPGMGGAQCLQKILALDPRARVIIASG
                     HRVNTQAEQDAVNGARAFISKPYNAHRMLRTIREVLEEGRRE"
     gene            complement(7412..8914)
                     /locus_tag="PLM79_06145"
     CDS             complement(7412..8914)
                     /locus_tag="PLM79_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV85924.1"
                     /translation="METIESRLIVPSRMSYLSPVLEYVRELARTVGFAEQEVRFIQLA
                     VEEAVTNVILHGYQGKPDAVFEVVCEQTAAGLTFSIREKGIPFDWTKLPEYSPDLARA
                     DQPVGGLGAHLMKSAMDEVAFLNHGRGGKEIRLTKYLGEKHIQTYPGGDVSRAQEIPA
                     DRSGPLRRPAWTVRELDPDEAVEVSRCAYRTYGYTYDDYIYYPERIRDLVHRGLLYSV
                     VAVTDSGQIIGHAALKKTHAEDPIAELGAFFIVPEFRGSTVFLRLCGFIKQIAPGLGL
                     RGFFARSVSAHTITQRGTAHFGLRDCGLLMGVPPEKIEFTNFSATPRRMGTRVLSFIS
                     VEEPRERVIYPPSELRDLVEKLYGNLNIPVVVGSTGEGASLALEGETELTSTRTMATN
                     VADIEVFRVGEHARDVVRRRCHLYRLEGAAVLFLYLDLEDPRTAALASDLLQDGFLLA
                     GILPLRMRGRDALILQYLNNLKVSYDLIHLHSSLAKDLLAHVRDHDPAPA"
     gene            complement(9077..10057)
                     /locus_tag="PLM79_06150"
     CDS             complement(9077..10057)
                     /locus_tag="PLM79_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV85925.1"
                     /translation="MSRPLRIEYPGAWYHVMNRGRRRERVFEEDADYRSFIALLREAA
                     TLWEAEVSAYCLMPNHYHLLIRTPLGNLSRCMRHINGVYTQRFNRAHGCDGPLFKGRF
                     KAVLVQGDSFLLQLVRYIHRNPVRAGMVDRPDRYRWSSHLGYLSATDHWNWLYKDFLL
                     SLLSPRKKDQRAAYRRFMGMEDTEDLLRKISGERWPPLLGDESMVNRLKARFFEEKVH
                     PQVPDSRTLAPDVDTIVQAVCSHYGVDRSELLRSRRGRFNEARGVAVHLVRLVRKESF
                     ADIGPVFGLRTDSAVGSVLDSMRKRLASDPELSERCRRILSALYKSYTET"
     gene            complement(10113..11636)
                     /locus_tag="PLM79_06155"
     CDS             complement(10113..11636)
                     /locus_tag="PLM79_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOV85926.1"
                     /translation="MIAKVHTCSLLGIDAILVEVEVDLSPGLPGFSTVGLPDNIVRES
                     KDRVKTALHNSGYPFPSERITVNLAPAHLKKEGAGFDLPIALGILAAMGMVHPQRARE
                     VAVVGELSLDGRVKPVAGSLPIAIQARRSGYAELLLPRSNACEAAVAEGLSVLPVGHL
                     SDAVEFLNGRASIEPARFDAQGIWNTDPAWEMDFDEVKGQEHAKRALEVAAAGGHNAL
                     MLGPPGAGKTMLARRIPGILPLLSFEEALETSKIFSVAGQLHEPLMVVRPFRAPHHTI
                     SDAGLVGGGHIPRPGEVSLAHNGVLFLDEFPEFRRNVLDLLRQPLEEGSVTIARAAMS
                     LTYPSRFMLIAAMNPCPCGYAGDPVRTCLCSPHQVLRYRGRISGPILDRIDLHIEVPA
                     LKYEELHSTEPAEPSARIRERVIACRERQLERFAGQGIYCNALMKPKAIKSHCRLDST
                     GQSLLEEAIRRLGLSARAYHRILKVARTIADLEARESIAPHHLLEAIQYRSLDRALF"
     gene            11817..12473
                     /locus_tag="PLM79_06160"
     CDS             11817..12473
                     /locus_tag="PLM79_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOV85927.1"
                     /translation="MSTCYYKILGVSVQASQEEIKRAFRMLAFRWHPDRNPHDEVAAE
                     RFREALQAYETLADPLLRGKYDRANGHSRSRQKLRRSSRTFKESPRSTYQEILEGLFG
                     IRLDRGAAEYHSYDLRFDLQVPRSAVSSGLRERIDYCRSVYCPQCMTGGKRAPRKACG
                     ACLGSGEVEEVCSLHLWIPPGSEHGTRLRVSGGGDRVHPGRPPGDLVIFLHVVDLPNR
                     "
     gene            complement(12483..13100)
                     /locus_tag="PLM79_06165"
     CDS             complement(12483..13100)
                     /locus_tag="PLM79_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85928.1"
                     /translation="MNLNAVLPTLEKIAAFYDALKYGHEGVEGYRKSTDLAKFLPCAC
                     RLWDSGVLDPEKTVFLDLGCADGRVNLLMSYFVRLSVGVEIDPEILGEYGPRRRDLLE
                     ELHREKLVKPAGNVHLFSGDSLHPGTYERIHRSLGIRFRDVDLFYTYITLHDLFAQKI
                     VSEAKPGARYLVYGFHKVLPRYEGLDVVDPDVGGGGIAALYQVRR"
     gene            complement(13097..13588)
                     /locus_tag="PLM79_06170"
     CDS             complement(13097..13588)
                     /locus_tag="PLM79_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85929.1"
                     /translation="MVSIGEKIRRAVRCYEENGDMAHRHPVLSRFARELEERIRSSRK
                     AMERLGVVAVCRSCEEEEGGSCCGAGIENRYGVGLLFINLMLGAELPPQRLKPESCHF
                     LGPRGCCLPARHVLCVNYLCGRLREGLGPEKTAALQKVAGEELDCLFRFHETLKKILS
                     PQP"
     gene            complement(13593..15185)
                     /locus_tag="PLM79_06175"
     CDS             complement(13593..15185)
                     /locus_tag="PLM79_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOV85930.1"
                     /translation="MTRNSFSVCKMLTEELDPEKLHRKFLASLLELQNVERGSIWIKR
                     TDGYQCVEALGSQCEMVRGITIPFDRPSLVGWVIENQKMTIGEPGLDKRHYKEIEDQI
                     EVKSRLILCLPLFLKSGEVYGALQIIDTSAGGKRLNLKKEYLDLLQDIVDVGSIALSN
                     SLIYASQVKENQKLRHVLDQFRAEERLIGRSPAFLKAIKTAAEYAGTDFPVLLTGESG
                     TGKELVAREIHRLSRRNDRPFLVQNCSAIPDTLLESELFGYERGAFTGATRDKVGLFE
                     SADGGTVFLDEIGDMSFHLQARILRVVQDSEIKPLGSARPRRVDVRIISATNKDLREE
                     IEKEQFREDLYYRLNVLPLHLPSLRDRPEDIPLLLDHFLARESRRMGTTPKKLTRESL
                     EFLMEYPWRGNVRELENFVKHILVVADGEWIRLEDLGVHFTGGQLKGDGQGSRSAGRE
                     LPRRGEPQPEASADPAEGGRTPGTIAEKSAFEGQSWEEVERSYVLYLLDKHKWHITRA
                     AQEAGVNRSTFDSRMKKLGIRK"
     gene            15701..17515
                     /locus_tag="PLM79_06180"
     CDS             15701..17515
                     /locus_tag="PLM79_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOV85931.1"
                     /translation="MPGSMQLKLMLLLFAFVLVPLCTVGIFSVRTAEELILNMATNQI
                     EHVAADKAALLERWISERKADIQVLAGSSVLSLLDTGQMASYLESVRRHYKVYSDISL
                     VSTQGDPIYSSSGTAARGEKEPWFRESLRGALHASDISLNPDQTASFFRISAPLVGRA
                     GKIEGVLCATVGTNAILSDVLRISLGKTGECYLVNGEGTFLAHKEPRRILTQNIAQSE
                     SFKNIFDTRNRRITYIDYRGVEVIGASARVGGTDWALVVEQDRDEAFQSADKLRHYVV
                     LIVALSTLGALISAWLLSRYILLPIRKLSEAADCLARGEFDRVNIETNRSDEIGTLYS
                     AFEDMARQLEDRQSRLEERVTLREAELKETDVLLKQTRQAAARSQQLASLGRLAAGVA
                     HEIRTPLTSLKMFLEGLELDADMAPEAEADLKVAMIQIQRMEATIHRFLSFARPQDPV
                     FAEVDLKELMEDALLVVRPRANQQETFLRTAIPRGLPTVLGDRKQLGEALLNLMVNAL
                     EALGNRGELDVEVAPWTNPANKPDSDWVRIDIKDTGPGIEAENLPRLFDPFFTTKATG
                     TGLGLSIVAGTVQRHGGRVEVCSLPGEGTVFSVFLPAS"
     gene            17535..18989
                     /locus_tag="PLM79_06185"
     CDS             17535..18989
                     /locus_tag="PLM79_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV85932.1"
                     /translation="MEKILIVDDDEGLVHFLSRFFTKQGYEVRSSSSGPAALELVSGE
                     PFDLILLDYKMPGMNGLDTLRQIRRAQVKTPVIVMTAYGTTDTAIEAMKLGAYDYLLK
                     PFEREELHRLAMDALEVNRLMKEVVSFPGPTHRSSPRGAVRIVGSHRRMQEVYKLIGQ
                     VAGKDVTVLITGESGTGKELVARALYHHSHRKDKPFLAVNCAAIPETLFESELFGHER
                     GAFTGAERIHIGKFERCHGGTLFFDEIGDMSLSTQSKLLRAIQYGEFERVGGTQTLRT
                     DVRILAATNRSLEQAVEAGSFREDLYWRLKIISVHLPPLRDRVEDIPGLVDYFVSRFN
                     EEYRTTIRHMEGSAVERLKAYSWPGNVRELENCIRRAVLLAQGDVILEEHLKIGSGDP
                     HSSPEPGPDRLLAAIDRKLDELIPDLLQLSSEKGKMRESIIEMIEEALVSKILKQCGN
                     NQVKAARMLGVSRNTLRHRMKKFNLLPPADSAPS"
     gene            19294..19659
                     /locus_tag="PLM79_06190"
     CDS             19294..19659
                     /locus_tag="PLM79_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85933.1"
                     /translation="MDEGVKVREKDWSVTPQEGISREVERVNARLRHFRGVAAGVMKD
                     AVGIWTEIWELVQDPRSCREILEGEEPAQGRPPWGDTGPLLEKLHLLGIHLEYARKLC
                     EGSIGNESQDEKGQLWPSS"
     gene            19644..19946
                     /locus_tag="PLM79_06195"
     CDS             19644..19946
                     /locus_tag="PLM79_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85934.1"
                     /translation="MAKQLTVISGNKIGGQYPLPVPDQRLKNFRAVMGVVLDDAQKTW
                     GEIWKEFQGQVTEGVLVLPEAEQGFKPPCGWPEFLERMWLLKHYLDYAKRFADGEV"
     gene            20130..21389
                     /locus_tag="PLM79_06200"
     CDS             20130..21389
                     /locus_tag="PLM79_06200"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOV85935.1"
                     /translation="MSGGNNHDNPFKIAQQQIQACADILDLPQSTRQVLLHPMREFHV
                     NFPVRMDDGAIRVFEGFRVQYNDAKGPAKGGIRFHPEETIDTVRALAAWMTWKCSLLD
                     LPLGGGKGGVICNPKEFSPAELERVSRAYIRAIGPFIGPEKDIPAPDVYTNPQIMAWM
                     VDEYSSIVGKNQFGVITGKPLALGGSPGRGDATARGGMYTLREAARKVGLDLSKATMA
                     IQGYGNAGSYAASLAESMFGIKVVAACDSKGAVTCKDGLCVLKVAEHKANTCSVLGCE
                     GTVDLSNEELLQMDVDILVLAALEGTLTQKNAQKVKAKLIVELANGPTTPEADEILYN
                     KGIHVIPDFLCNAGGVTVSYFEMVQNAYMYYWDEKEIHEKLDKKMTRAYHEVFDTSKR
                     HGINMRKAAYVVAVQRVVDAMKVRGWV"
     gene            21425..24061
                     /locus_tag="PLM79_06205"
     CDS             21425..24061
                     /locus_tag="PLM79_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV85936.1"
                     /translation="MPLPEARMVSCGIPSLDEVIQGLRLGDNVVWQVDEPDNYLRVVH
                     PFMNRAVEDGRTVVHIRFAPHPTVLPPSLPVADEEVDPRYGFDFFSAQVNQIIEERGR
                     GVFYIFDNLSSLVADWATDELLANFFQVTCPYLHELDTIAYFALTRGQHAHTAVARIR
                     DTTQVLIDAYHVRGDLYIHPLKVWDRYSSQMFLPHRYEDGLLHPVFKSGEASTISLTA
                     SKTPLRLKADSIAPWDSVYRKLTQYDEEELSLLESTAEMQALKQELSQMILGNDPEFK
                     RLSNRYLSLTDLLSVRNRLIGSGRIGGKAAGMLLARNILLKTPGGLDFSQVLEEHDSF
                     YIGSDVFFTFLVNNGLFRLRLRLTRDSQILKEEFEQVEKRFLAGRFPDEILEQFRDLL
                     DYFGQAPIIVRSSSLLEDGFGNSFAGKYRSEFCANQGTPEERMEAFLRAVKLVYASSV
                     NPDALSYRKQRGLGEADEQMAILVQRVSGMRYRSYFFPTLAGVAFSHNLYRWTERIDP
                     NQGIIRLVFGLGTRSVNRLGNDYPRLIAVSHPQLRPEVGSKIAFYSQRGVDVLNLAQN
                     EFQSVPLADLLAPADYPNLGLYLSGMEDGYLKDSIVPRQEDPRKWVLTFNHLLSKTAF
                     IPIMKELLATLEAAYGYPIDTEFTAAPDSRGNLRINPVQCRPMKVPGAVEVVGTPRDL
                     PRSLVLFRTHRAIHGGVVNGIRTIVYIDPRSYSRETPLDLKRAMGRLIGELNRHPQMR
                     TRRFMMMGPGRWGSSNIALGINTTYSDINHTSVLVEIAREEAGHVPDVSYGTHFFLDL
                     VESGIIYLPLYPNDPEAHFNEDFFAGAPNALLELLPDAGDFERFLKVIDVPAVTGGNH
                     AQVIADPRSAAALCYLAPDLSS"
     gene            complement(24185..24775)
                     /locus_tag="PLM79_06210"
     CDS             complement(24185..24775)
                     /locus_tag="PLM79_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOV85937.1"
                     /translation="MRIFFTGVGGQGTLLATRLVGEAALEEGLPVVVSEIHGMAQRGG
                     VVESAVAVGNLSSPTIADGEADILLAFEPLEALRALPKCHGRTVVVANTVPIVPFTVA
                     VGQSTYPSMELIRSSLEAKVARVIWVDAVQTARRSGSERGANMVMVGVLAGTGKIPVS
                     GKSWRKAMERVLPGKVLEANWKAFQSGFDLGAPGSE"
     gene            complement(24817..>25658)
                     /locus_tag="PLM79_06215"
     CDS             complement(24817..>25658)
                     /locus_tag="PLM79_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV85938.1"
                     /translation="AVGRCFQVPYDPPTIFSAPELPARPPTLCPGCPHRATYFSVRKV
                     FGDEAVYPTDIGCYTLGVLPPLRMADFLVCMGSSVSTAGGFSRVLDRPVVAFIGDSTF
                     FHGGIPGLINAVSHDHRFLLVILDNGTTAMTGHQPHPGVEWTRRGRIEPAVRIEDVVR
                     GCGVKKVALVNPLQMKKTQEVLDDLRRQMKDPGVSVLIAKSPCPLFERRMTGKKQKIV
                     FKVQGDCDRCKRCLRELGCPAFVWEASAAGEECIRINEALCNGCAFCAQMCPSIKPEK
                     IEG"
     assembly_gap    25659..25758
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25759..26834)
                     /locus_tag="PLM79_06220"
     CDS             complement(<25759..26834)
                     /locus_tag="PLM79_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOV85939.1"
                     /translation="MDELLVPASNRKHLLLGNEAVVRGAMEGNVRFVAAYPGTPSSEI
                     IDRFSQLAEEAGVYVEYSVNEKVSMETACAAALSGVRSLCSMKHVGLNVAADAFMTLA
                     YVGVRAGMVMVVADDPSLHSSQNEQDNRAYAKMAGVPMLEPSTPEEAREMTLSGLDLS
                     EAYRIPCMLRTTTRVNHSRGPVRFGELPREKPTASFTKDPFNLVVVPMVGRKLRLALM
                     EKEKMLREEAEKSPFNFVTGQGENGIITSGVSWLYVQDALRDLGLEGKVKVLKLGFTH
                     PMPRKLVGNFLRGVGKVLVVEELEPFLEEAVKGIAQEEEIPVKIVGKGEQWIPRAFEL
                     DSVKVKRAVGRCFQVPYDPPTIFSA"
     gene            complement(26827..28158)
                     /locus_tag="PLM79_06225"
     CDS             complement(26827..28158)
                     /locus_tag="PLM79_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV85940.1"
                     /translation="MPIWEPQEECMSRDELQQLQLERLQATLNRVYRNVSFYRRRFKE
                     MDFRPEDDLTDLRDIRRLPFTTARDVSDSYPYEMFAVPLREVVRVHSSSGVMVNPRVV
                     GYTRQDLKVWSNLVARILSAAGVTQDDVIQVTFGYGLLTGGLGIHYGAERIGASVLPT
                     SVGRTERQIKIMQDFRTTVLVSTPSYALVIADKVEAVGMDPKRLSLRYCICAGEPWTE
                     SMRKEIEERLFVKATDNYGISEVMGPGVAGECLEQQGMHLQEDHFLAEIIDPETGQVL
                     EPGREGELVITTLAKEAFPVIRYRTGDLCSIHPDPCPCGRTFHRLGRIRGRSDDVVVI
                     KGINVIPQRIGDILEEIKGERPVYQLAATRRGHQDQLEIRVEVTDQLFYDKMRDQKTL
                     VDVMRRKVTNFIGITPRIKLVERGSLERVGGEVRLFVDRREQASGTEDQDG"
     gene            complement(28200..28931)
                     /locus_tag="PLM79_06230"
     CDS             complement(28200..28931)
                     /locus_tag="PLM79_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85941.1"
                     /translation="MLKVRTVHTYYGSIHALKGVSLHVRPGEIVTLIGANGAGKSTLV
                     NTICGLVPCRKGRIEFLGDSVGELPPEEIVRRGIALVPEGRQIFSTLSVQANLEMGGF
                     IHRRNGRQVAEDMDRMFRRFPILEQRRNQLAGTLSGGEQQMLAISRALMSRPRLVVLD
                     EPSMGLAPLIVKEIFHIIRELRDEGSTILLIEQNARAALQIADRGYVMETGKMVLEGD
                     GSMLLEHREVRRAYLGKGAREIWEA"
     gene            complement(28951..29730)
                     /locus_tag="PLM79_06235"
     CDS             complement(28951..29730)
                     /locus_tag="PLM79_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV85942.1"
                     /translation="MKSPNGDSSILELAEVRMNFGGVAALKGIRYRVPPGIVQAVIGP
                     NGAGKTTLFHCITGLLRPTDGEIFFRGRRIDDLPPHGIARMGISRTFQHVALFKEMTV
                     LENVMLGRHPRSSRGFLATGLRLPGMRREERNIRERSLEYLDFVGLGLDADLAAGALP
                     LGKQKILEIARALATEPQLLLLDEPAGGLNMRETEELGTLIRRICDQGTTLMLVEHDM
                     NLVMDISDRILVLHYGETLATGSPREIKDHPRVIEAYLGES"
     gene            complement(29727..30710)
                     /locus_tag="PLM79_06240"
     CDS             complement(29727..30710)
                     /locus_tag="PLM79_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85943.1"
                     /translation="MQRKDLRILLGTILCLAAFPWLVQPLKAVSHYVDVMVFAGIFSL
                     VTLGLSLLMGYAGQISLAQAAFFGIGAYTSGILTSRLGWDPWAAIPAGAGLAAAVAWL
                     VGIPALRLKGHYLAMATLGFGVIVHIILAEEVEWTGGPSGMVDIPGLKIFGWKLNSEI
                     AWYYLVWGVVLLTLIFCFHVIHSRIGRALRAIHEEEVAAEAMGVPTARLKLQVFVISA
                     VLASVAGSFYNHYVTCLNPGSFNLMWSMRFMLMVMVGGMQSLWGTLLGTVLLTFVGNE
                     WLHVFAEFEMVVYGSLLLVVALFLPKGLVSLIPRRLFPSSAEKSSPKDGNP"
     gene            complement(30723..31613)
                     /locus_tag="PLM79_06245"
     CDS             complement(30723..31613)
                     /locus_tag="PLM79_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV85944.1"
                     /translation="MITTDLLQYTISGVTIGVIYALVAIGYNIVYNVTEIINFAQGEF
                     VMLGGLLAVFFVGSLGFPIPLAFAAAVAGAGILGFAMERFVIRRARSASVLSLIIITI
                     ALSILLKGGAMLGWGKDPYSLPAFSRGGPIMAAGAAVQPQALWILAISGLVVGALTIF
                     FRRTVYGKAMLACADNPRAARMVGIPVRRMVLLSFILSASIGAAAGVIITPISLMEYD
                     RGALLGLKGFGAAALGGLGQFFGALVAGVLLGLAESFCAGYLSSGYKDAVALVILLLA
                     LFFKPEGLFGSREAAKLKRF"
BASE COUNT         6243 a        10005 c         9559 g         5991 t
ORIGIN      
        1 gggacgacct gcccgacggg ggcgcccagg tttttgccgc gaccatccgg gccgtccggg
       61 accgggtgcc cggatgcacc gtcgaggtgt tggtcccgga tttttcaggg gattcggaag
      121 ccctggacgt ggtgctggat gcccatcccg aggtcctcgg ccacaacctc gagaccgtcc
      181 tgcgcctcta cccccgggtt cggccccggg cccaataccg ccggtccctg gatcttctcg
      241 aggccgccaa aaagaagcgg cccgatttgc tcaccaagtc gggaattatg ttgggactgg
      301 gagagatccc ccgggagatt ttccgggtca tggaggacct tcgaggggtg cactgcgata
      361 tccttaccct gggccaatac ctgcgcccct cgatccggaa gctgcccgtc gagcggtatt
      421 accccccccg ggaattcgaa gctctgcgga aagcgggaat ggatgcggga ttccggtggg
      481 tggaagcagg ccccctcgtc cgaagttcct accacgccgc cacccccgtt caagcgttgc
      541 ggacctagat cacaggaaat cgtacctcag gccgaagcaa aggtagttgg accctccgtg
      601 gacgccgtca aacaggccga aaaccccgga tcgatgatgc aggcgggtga tgaggctcca
      661 ccggggatac ttgggcagac cgaaggtgaa ttcgaacatc aggtagttga ggaaccgggc
      721 ggattcctcc ccgtgcctct ggcgttcgat gacgggcacc tcggtggcat aggaaagacc
      781 gtctcctgca gcaaagctcg tatccaggta gcggtcccag gggaatttca gccagcggag
      841 ggtgagcagg ccgttgaact cccaattctc ctggatcccg aaatgcttca ccacttggcc
      901 ctcggcctcg agccggatca ggtcgcacca ggtatggagg ttctttcccc cgtaacggca
      961 aagagatagg aatccctgaa atcgttgtcg aaggtaatga aatcgtcgat actgcccccg
     1021 gtattctgac ccccgtagac ggtcatgaac caattgttct gtgcatggac ctgctgtgcg
     1081 aagaacatca tgagggcaat caacatcacg gcaagtcgag actgcatccc cacccccttt
     1141 ttgaaagtcc cggtctcatt cattcccgat ccgtcctatc cctgggtttc tgatttacca
     1201 tgaatgactt acggctccaa attgttttca ggcagggacc ctccctcgac gtctccggaa
     1261 ggcggaccgg accgcccccg gacccggaat cagccgcact cggattcccc ggcttcccgg
     1321 gccagtcgtt ccacctttcg cacataggcg tcgatgtcgc actttctctc gaggcccgcg
     1381 gcggacatgc tccgcacggt gccgtagacg gaccttcctc cccagggccg gtcgtgcagt
     1441 ccgaagatcc agcctgcatt ggcataggaa ttgggatcgc gcccgtcgat gaaataccgg
     1501 ttgttgagag ccaggagggt gcggtaggcc atctcgggac tcgggctcca ttccaggatt
     1561 ttctttcccc aatacatgcg catgtagttg tgcatgtagc cggtgacctt catctcgacc
     1621 atggccgcat tccagtatgg gtcatgggtc tgtgccctct cgagctgttc cgggccgtag
     1681 agagggttcc tcgggtcttt cgagtgtgca tcgagggttc gacgcgccca gtcgggcaga
     1741 caggagaagg catcgtaatc gggagtgaaa tgaacgaagt ttgcggccag ttcgcgccgc
     1801 acgatcagct catcgaggta ggcgtccttt ccctgctgca aagagggagg aagccgggtc
     1861 acccggaggg ccagctcgag gggagagatc tggccgaagt gcaggtacgg gctcatgtgc
     1921 gacacgtcgt cggtctgagg ctgatttcgg ttggcgggat acctcgagaa tcgcccgcgg
     1981 aggaattctt cgaaggtttc ccgggcggcc gtggcgcccc cccggaagaa gggagagacc
     2041 gttttcaggg aaggatccgc cggaaagttc ctccaggagg cccaatcctc cagctgctcc
     2101 gcctcgagcc ccgcgatggg cagagccagg gaggacttat atggaattgc ctgttccggc
     2161 tccaccaggt acttggaaag gtgccggtga atcttgggac gaagggtgcg ggctgcgtac
     2221 tcccgcttgt cggatgccgc ctccacggga accaccacgt ccgactcgac ctgctcgacc
     2281 ccgcagggga cctcccgtgc cacgtgagcc cgccatgcct tttgatggcg caaatacccg
     2341 caatcgcaaa cgaggaaagc ggcccttcgg cctaattccc gggccacttc ttcaggggga
     2401 cctcgacgaa tcaccatctt gacggaccgc cgggcaagga gccggtgggt ttccgcaagc
     2461 ccctccaaca ggaaggcgta atgacgcagg ttggcttccg ggtatcggtc ggtgatgccg
     2521 aaacaggcca ccaggggcct gttcagttcc tcggccctgc gcacggcatg ttccagggcg
     2581 tggttgtagt cggccctctg ggactgctgc atccagtaga gcacgtagtc tcccgggacg
     2641 gcgggtcgat cgttgagttt ccggattctt tcctcctgaa cctgttctgc gctcatggca
     2701 tcgacctttc gtggatatgc ccgagggacc atcccttcca tgacagagaa tccccgtctc
     2761 ggagcgagca ccgtctggtc ggcggctcac ctggaactat gacggggagc gtcgcttccg
     2821 ggaagcttcg gccctgccgg gtcatggggt tccttgctcc ggggacgata cccgtttcgc
     2881 cttcgccatc cgctccgccg ttattttctt gaatcgctcc atcctgtttt gcgggggagt
     2941 gaatcgctcg ttttccgatt tttgttcgag gcgcaacgca ccctcggggc aggtgggaac
     3001 gcaaaggccg cagccgatgc accgttcccg caagacaacg gccgttgccc cgtccatccg
     3061 gacggcctcc atctggcagc gactcacaca ggtctcgcac ccggtgcagc attccccgtc
     3121 caccacggcg aaaaagttcg tggcggccac ccgggcgggg gcggggagcg cttttaggtt
     3181 cttgagtatc tggcagcagc acccgcagca ggtgcagata ttgactacct gttggctgtt
     3241 ggagggctga agaaccagtc cggcctcctc cgcccggtcc aggatctgaa aggcttcccg
     3301 gtggtcgatg accctcccga gcccgttttc ttcgtaatac tgggccccta ccccgaacac
     3361 aaagcagctc tcgagggggc gaccgcaacc cttgcccagg atctggtgct cctttctgca
     3421 gatacaggga gcgatcagga tcttctcctg ccccgcaagg atctttcgag cctcttcgta
     3481 aggcagaatt tcttcccggg attcgatggc ccggtcgatg gggatggttc ggagctgggg
     3541 gcttttttgt tggctgtctt gacggaagaa ataaggaatg tattcgttca catcccggat
     3601 gagctccggg tccaggtcgt tcacatggaa ttcccagatt ccaatgatga actgggcggc
     3661 catgtagtac acctgttccc ctttgcggag gcgatagatc agccccttgc gggacatttc
     3721 gtacagcctt cgagccgctt cccgggggtc caaaccggct cggacggcga tggcctcggg
     3781 aggctcgggt ctcagggtcg tcatcaccgc cagcgcggct tcttcgggtg tgaagagcct
     3841 gcgaaggatt cgcagttcca cccccgtggg ggtccgagga tagccctcgg gaaggcggtc
     3901 caggtgggcc gccaaggcct catacacctt tgaaagatcg ttcatggaga tattctccgg
     3961 tcgggtgaaa tggttgtcgg tgagatcctc ccggttgttc cacggggtgg aaccctttcc
     4021 aagacccgat cccccctcct cttcctcggt tcctgctttt ttttggtgcc gggaactctc
     4081 gtttcggtca tataatgcgt tcggtaaggg acttgcaaac cctttgcctc aactccctaa
     4141 catggattcc atatgaacgg tgagctcaga ccggataagg agttcaagga cgacattctg
     4201 cgggaacaaa tccgcctgtc catgaagcag cttccgacca tgcaggcggc ctccttcgtc
     4261 gtcgccatcg tcctggctta ttccgtgcgc catatcgttc ccgcctcgaa cattctggcg
     4321 tggctcttga tgattttggc cgtcgtcgcg agcaggacca tcctctatta ccgattcgtc
     4381 agggtgcggg agcaatcctt tgacggggaa tactggaaaa agctcttcct ggtgctggcc
     4441 ctgatttcgg gggtgctctg ggggatttcc gccttcattg tctttcccgc ggaccagcaa
     4501 gggctgatat ttcttttcgt gctggtaatg gcgagccttt ccgcggccac caccgtatcc
     4561 cattccccca tccgactggg ccccacggcc tgggccgcac ccgcgatgct gctgtatgcc
     4621 gttcgttgct tcgccgacgg aggagagttg ggctataccc tgtgcgccct gatccttctt
     4681 tacctgttca ccgtcctttg ctattccttc caccagaatc gcgcggtcac gtccgccatt
     4741 tccctgagat tcgaaaacct ggggcttctc gcagaacttc gcaatgcccg ggaactgctc
     4801 gagaagaaag tcgaggaacg caccgccgaa ctgaaacgga ccaacgaaac ccttagccgg
     4861 gaagtcgagg aaagacgctc catcgaggct gccctgcgcc tgagcgagaa aaaataccgc
     4921 gacctggtcg agctcgccaa cagcgtcatc ctacggtggg atcccaaggg caccataacc
     4981 ttcgccaaca aatttgccca gcaattcttc ggcttcgggg aagaggaact cgtgggaaag
     5041 agcatcctgg aaaccatcgt ccccccgcgc tccccggaag ggggcgacct ggaagaagtg
     5101 atccgggaga tctgccggac cccccacgga ttcgagacaa gagaacgcga gaaccttcgc
     5161 aagaacggag agagagtttg ggtcagttgg acccacaggc ccatctattc cgagaacggg
     5221 gacctggtgg aattgctggg catcggccag gacacgaccc agcgcagact gaccgccgag
     5281 gctctccggg tacaggagga gcgtcttcgt ctggccatgg cggcttcccg gcagggctgg
     5341 ttcgatctga acgtccggac gggagaagtc gtcgccagtc ccgaatacgc ccggatcatg
     5401 ggctatgaac cctccgagtt cagctcgaac ctgcgcgcct ggatggaggg gattcatccc
     5461 gatgatcggg acaaggtcct cgatgcattc aacgaatgca tcgagaccgg agagacgcga
     5521 agcatggagt accgtcaccg gaccaagacc gggcaatgga agtggatccg gtccaccgga
     5581 aagctcgtcc agtacgattc ccaagagaaa cccctgcgaa tgatcggtac ccacgcggac
     5641 atttccgagc gcaaacaagc cgaagaagcc ctgcgggaga gccaggaaag atttagagaa
     5701 ctgacggaat tgctccccga aaccatcttc gaagcggacg tccaggggac tctcaccttc
     5761 gtgaaccgga gcggcatgga tcatttcgga tattcccagg aggacttcga ggggggtctg
     5821 aattgcttcg atatgatcgc tcccgaagac cgcccccgga ccctggaaaa cgccgcaaga
     5881 ctgctgagcg gaggttcaat cgggctcaat gaatacaagg ccttgagaaa agacggaacg
     5941 gttttccccg ccatcatccg ctccagtgcc aaggtccgtg acgggaaggc ggtgggattt
     6001 cgaggcatcg tcatcgacat taccgaaacc aagaggctcg aagaacagct tcgacaggcc
     6061 cacaagatgg aagcggtggg caccctggcg ggcggcatcg cccacgattt caacaacctt
     6121 ctccagacca tcctgggttt ttccgagctc ctgctgctgg aaaagacgga agaagaggaa
     6181 gggtacccgg aactccagga gatctttcgg gccgctgaac ggggagcgga gctcattcgc
     6241 cagcttctcg ccttcagcag caagatcgaa tcgaagctcc agcccgtgaa tctcaaccag
     6301 gtcgtggaga atgtaagggt gctgctggaa cggaccattc ccaaaatgat ccggatcgag
     6361 ctccgcctcg cagaagacct tcaatacgtc aatgccgacg ctccccagat ggagcaggtg
     6421 ctggtcaatc tcggggtcaa tgctcgagat gccatgcccc aggggggcac cctcaccatc
     6481 gagaccgaaa atgtcgttgt ggacaaggaa catcatcgag cacatcccga gctgcttccc
     6541 ggacactatg tgctcttgac ggccacggac agcggccagg gaatggacgg gaccatccga
     6601 gaacacatct tcgaaccgtt tttcaccacc aaagaggtcg ggaaagggac cggcctgggg
     6661 ctggccatgg tctacggcat catcaagaat cacagcggtc acatcttctg caccagcaac
     6721 ccgggccaag ggacccggtt ctccatctac ttgcccgcaa ctcaaccggt cgaagctttg
     6781 ccctatagga tggaagaagg cgaggaaccg cggggcggca acgaaaccat cctgctggtc
     6841 gatgacgagg attccatccg cgattttgcc cgaaggatcc ttgaaaccca cggctacagg
     6901 gtgatccccg cccccagcgg ggagaacgcc ctgggagtct atcaaaaaga cggaagcgga
     6961 atcgacctgg tcattctgga cctgatcatg cccggaatgg gcggtgccca gtgcctgcaa
     7021 aagatcctcg ccctggaccc acgggcccgg gtgatcatcg ccagcggtca ccgggtgaac
     7081 acccaagcgg aacaagacgc ggtaaacgga gcccgggctt tcatcagcaa accctacaac
     7141 gcccaccgaa tgctcaggac cattcgagag gtgctggagg aaggcaggcg ggaatgagtc
     7201 gagccccggg gcgacggagt catctccatc cccctcggca acccctccag aggctccagc
     7261 cgcgagctct cagtatctgg gaccggtaca cggtcatctt cgtgctccct ttgccgtccg
     7321 ccgtcttgag ggtcacaacg gccttgcggc ctttccccgt agcattcctg tctaccgtga
     7381 cgcggggacg ggtgttctcg cgatccccgg gttacgccgg ggccgggtcg tgatcccgaa
     7441 cgtgggcaag aagatccttg gccagggaag aatggagatg aatcaggtcg tagctcacct
     7501 tcaagttgtt caaatactga aggatgagcg catcgcggcc cctcatccgc agagggagga
     7561 ttcctgcgag gaggaacccg tcctgcaaca gatccgaggc cagggccgcc gttcgagggt
     7621 cctccaggtc caggtacaga aacaggaccg ccgccccctc caaccggtag agatggcatc
     7681 gccgccgtac cacatcccgg gcatgttctc ccaccctgaa aacctcgata tcggcaacgt
     7741 tggtcgccat ggtccgggtc gaggtcaatt cggtctcccc ctcgagggcg agggaggccc
     7801 cctccccggt cgatcccacg accacgggga tgttcaggtt cccgtagagc ttctccacca
     7861 ggtcccgcaa ctcactcggc ggatagatga ctctttcccg aggctcttcc acggaaatga
     7921 acgacaatac ccgggtgccc atccgccgcg gggtggccga aaaattcgtg aactcgatct
     7981 tttccggggg aactcccatg aggaggccgc aatcccggag accgaaatgg gccgtgcccc
     8041 gttgcgtgat ggtatgggcg ctgacggacc gcgcaaagaa tccgcgaagg cccaggcccg
     8101 gggcaatctg tttgatgaac ccgcaaagtc gaagaaaaac cgtactgccc cgaaattccg
     8161 ggacaatgaa gaacgccccc agttctgcga tggggtcttc cgcgtgagtc ttcttcaggg
     8221 cggcgtgacc gatgatctgg ccggaatcgg ttacggccac gacggaatac agcaatcccc
     8281 ggtgcaccag gtcccggatc cgctccggat aatagatgta gtcgtcgtag gtgtacccgt
     8341 aggttcgata ggcgcaccgg gaaacctcga cggcttcgtc cggatcgagc tccctcacgg
     8401 tccaagcggg acgccggagg ggcccgctcc ggtccgcggg gatttcctgc gcccggctta
     8461 cgtccccgcc cggataggtc tggatgtgct tttcgcccag gtacttggtc aggcggatct
     8521 cttttccccc tcgtccgtga ttgagaaagg caacttcatc catggcggat ttcatgagat
     8581 gcgccccgag gcctcccacg ggctggtcgg cccttgcgag gtcgggggaa tattccggga
     8641 gcttcgtcca gtcgaaagga atcccctttt cccgaatgga aaaggtcaag cccgcggccg
     8701 tctgctcgca cacgacttca aagaccgcgt ccggttttcc ttggtagccg tgcagaatca
     8761 cattggtgac ggcttcctcg acggccagtt gaatgaagcg gacttcctgt tccgcaaaac
     8821 cgacggttcg agccagttcg cgaacgtatt cgaggacggg cgacaggtac gacatgcggc
     8881 tgggaacgat cagccgggat tcgatggttt ccatggcgga cgggaccttt cgacattgaa
     8941 ccttgccctc tcccggcttc gggcccaacc cggggtagcg gcccgacggg cggagaagga
     9001 aacatggggc ggccgccccc cccgaccccc cgacgacaag actgcaaccg gcccggggct
     9061 tcgcgcggaa ctggggtcaa gtctccgtat agcttttata gagggcactg aggatgcgcc
     9121 ggcaacgctc gctcagttcc gggtcggatg ccaggcgctt tcgcattgaa tccaacacgc
     9181 ttccaacggc actatccgtt cgaagaccga agacaggacc gatgtcggca aagctctcct
     9241 ttcgcaccag tcgaaccagg tggacggcca cgccgcgagc ctcattgaac cgcccccttc
     9301 gggacctgag cagctccgat cgatccaccc cgtaatgaga gcagacggcc tgcacgattg
     9361 tatcgacatc cggtgcaagc gtgcgcgaat cgggcacctg cgggtggacc ttctcctcga
     9421 agaaccgtgc tttcaagcga ttcaccatgc tttcgtctcc aagaagaggc ggccatctct
     9481 caccgctgat tttccgcaac agatcctccg tgtcctccat gcccatgaac cgtcggtagg
     9541 ctgccctttg atccttcttc cgaggcgaaa ggagggaaag gagaaagtcc ttgtagagcc
     9601 agttccaatg atccgtggcg gatagatacc ccaaatgact gctccagcgg taacgatccg
     9661 ggcgatccac catcccggcg cgaaccggat tcctgtgaat gtagcgcaca agctgcagaa
     9721 gaaaagaatc cccttgaacc agaacggctt tgaaccgtcc cttgaacaac ggtccgtcac
     9781 acccgtgagc cctgttgaag cgctgggtgt agaccccgtt gatgtggcgc atgcaccggg
     9841 acaggtttcc caaaggagta cggatcaaga gatggtaatg gttgggcatc aggcagtaag
     9901 cgctcacctc ggcttcccac aacgtggcag cttctctcag gagggcgatg aagctgcgat
     9961 aatccgcatc ttcttcgaac acccgctccc gccgacggcc ccggttcatc acatgatacc
    10021 aggccccggg atattcgatg cgtaagggtc tgctcatgaa acgacgctac tccgatccat
    10081 aataaaagtc aaacggagac ctgaccccgc tttcaaaaca gggcgcggtc cagggagcgg
    10141 tactggatgg cctcgaggag gtgatggggg gcgatggact cccgggcttc gaggtcggcg
    10201 atggtccttg ccaccttcaa gatgcggtga taggcccggg cactcaggcc caggcgccgg
    10261 atggcctcct ccagcaggct ctgcccggtc gagtccagcc gacagtggct cttgatggcc
    10321 ttgggcttca tgagggcgtt gcagtagatg ccctggcccg caaatcgttc gagctgcctt
    10381 tcccggcatg cgatcacccg ttcccgaatg cgggcggagg gctccgcggg ctccgtcgaa
    10441 tggagctcct cgtacttgag ggcggggact tcgatgtgaa gatcgatgcg gtccaggatg
    10501 ggccccgaga tgcgtccccg atagcgaagc acctggtggg gggaacacag gcaggtccgc
    10561 acggggtctc ccgcgtaccc gcacgggcac gggttcatgg ccgcaatgag cataaagcgg
    10621 gacgggtagg tgagggacat ggcggcccgg gcaatggtga cgctgccctc ctccaggggc
    10681 tgcctcagca ggtccaggac gtttcgccgg aattccggga actcgtccag gaagagaacc
    10741 ccgttgtgag ccaggctcac ctcccccggc cgcgggatgt ggccgcctcc caccagcccg
    10801 gcatcggaga tggtgtggtg aggggcccga aacggccgca ccaccatgag gggttcgtgg
    10861 agttggcccg ccacgctgaa gattttggag gtctcgaggg cctcttcgaa ggaaagaagg
    10921 ggcagaattc cggggattct tcgggccaac atggtcttac ccgccccggg gggacccagc
    10981 atcagggcgt tgtgcccgcc ggccgccgcc acctccagag ccctcttggc gtgttcctgc
    11041 ccttttactt cgtcgaaatc catttcccat gcggggtccg tgttccagat cccctgggcg
    11101 tcaaaccggg cgggttcgat ggaagcgcga ccgttcagga attccaccgc atcggacagg
    11161 tggcccacgg gcagcacgga gagcccttcg gccacggccg cttcacaggc gttgctccga
    11221 ggcaggagca gctccgcgta cccgctcctg cgggcctgga tggcaatggg gaggctcccg
    11281 gccaccggct ttacccgccc gtccagggaa agttccccta cgaccgccac ctcccgggcg
    11341 cgctgtgggt gaaccatccc catggccgcc aggattccca aggcgatggg aaggtcaaac
    11401 cccgcccctt cctttttcag gtgggccggg gccaggttca cggtgatccg ttccgacgga
    11461 aaagggtacc cgctgttatg aagggccgtc ttcacccggt ccttgctctc gcgaacgatg
    11521 ttgtcgggca aacccaccgt cgagaaaccg ggaagtcccg gggaaaggtc cacttccacc
    11581 tccaccagga tggcgtcgat gcccagcaag ctgcacgtat gaactttggc aatcatttcg
    11641 gggcggactt tccacgaaga tccggtgcgg ggcctgattc ttccttgttt tgtcaaatca
    11701 ttgtttttac aatacccacg tggttgccgg gatttcgccc caatcgagca agacatcacg
    11761 ggccggaaaa tcggcatccg gcgggagaca gtcagccagt tgctgaagcg gtcgagatga
    11821 gcacttgtta ttacaaaatt ttaggggttt ccgttcaggc cagtcaagag gaaatcaagc
    11881 gggcctttcg aatgctggca ttccgctggc atccggaccg caatcctcac gacgaggttg
    11941 ccgcggaacg gtttcgggag gcgctgcagg cctatgagac cctggcggat cccctcctgc
    12001 gcggcaagta tgaccgggcc aacgggcact cccgttcccg gcaaaagctt cggcgctctt
    12061 caaggacttt caaggaatcc ccccgttcaa cctaccagga gatattggaa gggctcttcg
    12121 ggattcgcct ggaccggggt gccgctgagt atcattccta cgatctgcgg tttgatctgc
    12181 aggtgcctcg cagcgccgtt tcgagcggat tgcgggagcg catcgattac tgccgttccg
    12241 tctactgtcc ccagtgcatg accggtggaa agagggctcc ccgcaaggcc tgcggcgcct
    12301 gcctgggcag cggagaggtg gaggaggtct gttcgctcca cctgtggatt ccccccggat
    12361 cggaacacgg gacccgattg cgcgtttcgg gagggggaga ccgggttcac ccgggaaggc
    12421 cgccgggaga cctggtcatt ttcctgcacg tggtcgatct tcccaaccgg tagtcccgcc
    12481 cgtcaacggc gcacttggta cagagccgca attcccccgc cccccacatc gggatccaca
    12541 acatccaacc cttcgtagcg aggaaggacc ttgtgaaagc cgtacaccag gtagcgggcc
    12601 cccggctttg cctcggacac gatcttttgg gcaaagaggt cgtggagggt gatgtacgta
    12661 tagaacaggt ccacatcccg gaagcggatc cccagggatc tgtggattcg ttcgtaggtg
    12721 cccggatgca gcgaatcccc cgaaaacagg tgaacgtttc ccgcaggctt caccagtttc
    12781 tcgcggtgca actcctccag gagatctctc cttcgcggtc cgtattcccc gagaatttcc
    12841 gggtcgattt ccaccccgac ggaaaggcgg acaaagtagc tcatgagaag gtttacgcgg
    12901 ccgtcggcac atcccaggtc caggaagacg gtcttctcgg ggtcaaggac gccggagtcc
    12961 cacaggcggc aggcacaggg aaggaacttg gccagatccg tggactttcg gtagccctcg
    13021 accccttcgt gaccgtactt tagggcatca tagaaggccg cgattttctc cagcgtcggc
    13081 aggacggcgt tcaggttcat ggttgggggc tcaagatctt tttcagggtt tcgtgaaacc
    13141 ggaaaaggca atccagctct tccccggcga ccttttgcaa ggcggccgtt ttttcggggc
    13201 cgagaccttc ccgaaggcgg ccgcacaggt agttcacgca gaggacatgg cgggccggga
    13261 ggcagcagcc ccgaggaccc agaaaatggc agctttccgg ttttagccgc tgtgggggaa
    13321 gctcggcgcc cagcatgagg ttgatgaaaa gcaggccgac tccgtatcga ttctcgattc
    13381 cggctccgca acagcttcca ccctcctctt cttcacagga tctgcacacc gcaaccactc
    13441 cgagccgctc catggccttg cgggaagacc gaattctctc ctccagttcc cgggcaaacc
    13501 gggacaggac cgggtgtcgg tgggccatgt ccccgttttc ttcatagcac cgcacggccc
    13561 gacggatttt ttccccgatg gagaccatcg ttctactttc ttattcccag ctttttcatc
    13621 cgggaatcga aggtggaccg attgacccca gcttcctggg ccgcacgggt gatgtgccac
    13681 ttgtgcttgt ccaggaggta caagacgtag gatcgttcga cctcttccca ggattggcct
    13741 tcaaaggcgg atttttccgc aatcgtcccg ggggtcctgc ccccttctgc gggatcggcc
    13801 gaggcttcgg gctggggctc gccccgcctg gggagctcgc ggcccgcgga tcgagacccc
    13861 tggccgtccc ctttgagctg tcctccggta aaatgaacac ccaggtcttc gagcctgatc
    13921 cactccccat cggcgaccac aagaatgtgt ttgacgaaat tttccagctc ccggacgttt
    13981 cccctccagg gatattccat gagaaactcg agggactccc gggtgagctt cttgggggtt
    14041 gtgcccattc gccgggactc tcttgcgaga aaatgatcca ggagaagagg aatgtcttcc
    14101 gggcggtccc gtaggctggg caggtggagg ggcaagacat tgaggcgata gtaaaggtcc
    14161 tcccgaaact gctccttttc gatctcttcc cgcaggtcct tgttggtggc ggagatgatc
    14221 cggacatcca ccctccgggg ccgcgcggat cccaacggtt tgatctcgct gtcctggacg
    14281 acccgcagga tcctggcctg gaggtgaaag gacatgtctc cgatttcatc gaggaagacc
    14341 gttccgccgt cggcggattc gaaaagaccc accttgtccc gggtggcgcc ggtgaaggcc
    14401 ccccgttcgt agccgaaaag ctcgctttcc agcagggtat cgggaatggc gctgcagttt
    14461 tgcaccagga acggccgatc gtttcgtcgg ctcaaacgat ggatctcccg ggccaccagc
    14521 tcttttcccg tcccgctctc ccccgtcagg agcacgggga aatccgtccc ggcgtattcc
    14581 gcggccgtct tgatggcctt cagaaaggcc gggcttcggc cgatgagccg ctcctcggcc
    14641 cggaattgat ccaggacatg acgaagcttc tggttctcct tgacctggct cgcgtagatg
    14701 agggagttgc tcagagcgat ggagcccaca tcgacgatgt cctggagcag atccaggtat
    14761 tcttttttga gattgaggcg cttgcccccg gcgctggtat cgatgatctg gagggcaccg
    14821 tagacttcgc cgctcttgag gaaaagggga agacacagga tcaggcgact cttgacctcg
    14881 atctgatcct cgatttcctt gtaatggcgc ttgtccagcc cgggctcccc gatggtcatc
    14941 ttctggtttt caatgaccca tcccacgagg ctgggacgat cgaagggaat cgtgatcccc
    15001 cggaccatct cgcactggct tcccagggcc tcgacacatt ggtaaccgtc ggttcgtttt
    15061 atccagatgg aaccgcgctc gacgttctgg agttccagca aagaggcgag gaacttgcga
    15121 tggagctttt cggggtccag ttcctcggtg agcatcttgc aaacactgaa ggaattccga
    15181 gtcataggtc cactcccgct ccccttgctc cctttgcgac gcttccgcaa ataccgggac
    15241 ccgcggatcg gggagttctt ccggggatca accctggttg ctgccggtca caataccaat
    15301 gagagttctt gcttgggtga aattgcggtc tgaccgcgag accgatccac aaacaggcag
    15361 accggacgaa tgaggtttta tccttgctga tccgaggggc ggtcgtcaag ctcaaaagag
    15421 cctccgacgg cggacccggc ggattccggg gatctagggg gaacggttgg agcgggaccg
    15481 cgatggttcg ggcctcccca gcggaggttg tctccaggca aaaactcggg catagtagtg
    15541 aattatcgag aaattcgttc ccgacgctcc ctacccccgc ccccggggaa aagggacgag
    15601 ggggaagctt tgcaggaccg tcggtccctg cgccccgagc cggggagggt ccgccatggg
    15661 tcggcgaggg agagcggcct cgccgacgac gaggttgccg atgccgggaa gcatgcagct
    15721 caagttgatg ctgctcctct tcgcattcgt tttggtgccc ctttgcaccg tggggatctt
    15781 ctccgttcga acggccgaag agctgatcct caacatggcc accaaccaga tcgagcacgt
    15841 ggcggcggac aaggccgctt tgctggaacg gtggatctcc gagcggaaag ccgacatcca
    15901 ggtgctggcg ggctcctcgg tcctgagcct cctggacacc gggcagatgg cttcctacct
    15961 cgagtccgtt cgtcggcatt acaaggttta cagcgacatc agcctggtat ccacgcaagg
    16021 agatcccatc tacagcagtt ccggcacggc cgcacggggg gagaaggaac cctggttccg
    16081 ggaatccctt cggggcgcac ttcacgcctc ggatatctcc ctgaacccgg accagacggc
    16141 ttctttcttt cgcatatcgg ccccgctcgt ggggcgagcg gggaagatcg agggcgtttt
    16201 atgcgccact gtgggcacca acgccattct ttcggacgtc cttcgcatct ccctgggaaa
    16261 gaccggagaa tgctacctgg tcaacggcga agggaccttt ctcgctcaca aggaaccccg
    16321 gagaatcctc acccagaaca tcgcccagtc cgaaagcttc aagaatatct tcgacacccg
    16381 gaatcggcgg atcacctaca tcgactatcg aggcgtggag gtcatcgggg cttcggcccg
    16441 ggtgggaggg acggactggg ccctggtggt ggaacaggac cgggacgagg cgtttcagag
    16501 cgccgacaag ctgcggcatt acgtggttct catcgttgcc ttatcgacct tgggcgccct
    16561 catttccgca tggctgcttt cccgctacat tctgctgccc atccgcaagc tgagcgaggc
    16621 cgccgactgc ctggcccggg gagaattcga ccgggtaaac atcgaaacca accgctccga
    16681 cgagatcggg accctctaca gcgccttcga agacatggcc cggcagctcg aggatcggca
    16741 aagcaggctc gaggagcggg tgactctccg ggaagccgaa ttgaaggaaa ccgacgtcct
    16801 tctgaagcag acccggcagg ccgcggcccg atcccagcag ctggcctccc tgggccgcct
    16861 cgccgcgggc gtggcccacg agatccggac ccccctgact tccctgaaga tgtttctcga
    16921 gggcctcgaa ctcgatgcgg acatggcccc ggaagcggaa gcggacttga aggtggccat
    16981 gatccagatc cagcgaatgg aggccaccat ccatcgtttt cttagttttg cccggcccca
    17041 ggacccggtg tttgccgagg tggacctcaa ggagctcatg gaggatgctc tcctcgtggt
    17101 gaggccccgg gccaaccagc aggagacctt tcttcgaacc gcgattcccc ggggcctccc
    17161 gaccgtcctc ggggatcgaa agcaactggg agaggccctt ctcaacctga tggtcaacgc
    17221 tctcgaagcc ctggggaacc ggggggagct cgacgtcgag gtcgccccct ggacgaatcc
    17281 ggcaaacaaa ccggattccg actgggttcg catcgatatc aaggacacgg gaccgggaat
    17341 cgaggccgag aacctccctc ggcttttcga cccgttcttc accaccaagg ccacgggaac
    17401 cggcctggga ctttccatcg tcgccggcac ggtgcagagg cacggcggcc gagtcgaggt
    17461 ctgcagcctt cccggggaag ggaccgtatt ttccgtgttc cttccggcat cctgaaattg
    17521 gaggcacaaa gaacatggaa aaaatcctga ttgtggatga cgacgaagga ctggtgcatt
    17581 tcctttcccg attttttacc aagcagggtt acgaagttcg ctcctcgagc agcgggcctg
    17641 cggccctgga actggtctcg ggggagcctt tcgatctcat cctcctggac tacaagatgc
    17701 cgggaatgaa tggcctggac accctccggc agatccgccg ggcccaggtc aaaacccccg
    17761 tcattgtcat gaccgcttac ggcaccacgg acaccgccat cgaagccatg aagctgggag
    17821 cctacgacta cctgctcaag cccttcgagc gcgaagagct gcaccgcctg gccatggacg
    17881 ccctcgaggt gaaccgactc atgaaggaag tggtgagctt tcccggaccg acccacaggt
    17941 cctcgccccg gggagcggtc cggatcgtgg gcagccaccg gcgaatgcag gaggtctaca
    18001 agctcatcgg ccaggtggcg ggcaaggacg tcaccgttct cattaccggg gaatcgggaa
    18061 ccggcaaaga gctggtggcc cgggccctct atcatcacag ccaccggaag gacaagccgt
    18121 ttctggccgt caattgcgcg gccatccccg aaaccttgtt cgaaagcgag ctgttcggac
    18181 acgagcgggg agccttcacc ggggccgaac gaatccacat cgggaaattc gagcgttgtc
    18241 acggcggcac ccttttcttc gacgaaatcg gcgacatgtc cctttccacc caatccaagc
    18301 tgctgcgcgc catccagtac ggagagttcg aacgggtcgg gggtacccaa acccttcgaa
    18361 cggatgtcag gatcctcgcc gccaccaacc ggagcctcga gcaggccgtc gaggcgggga
    18421 gttttcggga agatctttac tggagactca agatcatctc ggttcatctg ccccctctcc
    18481 gagaccgggt cgaagacatt cccggcctgg tggattactt cgtcagtcgt ttcaacgagg
    18541 aataccggac gaccatccgc cacatggaag ggagcgccgt ggaacggctg aaagcctact
    18601 cgtggcccgg caacgtgcgg gaactggaaa actgcatccg gcgggcggtg ctcctggccc
    18661 agggggacgt catcctcgaa gaacacctga aaatcgggtc gggcgacccg cattcgtccc
    18721 cggaacccgg gccggaccgg ttgctggccg caatcgaccg caagctcgac gagctcattc
    18781 ccgatctttt gcagttgtcc agcgagaagg ggaaaatgcg cgagagcatc atcgagatga
    18841 tcgaggaggc cctggtctcg aagatcctga aacagtgcgg aaacaaccag gtgaaggccg
    18901 caagaatgct gggcgtcagc cgcaacaccc tgcgacaccg catgaagaaa ttcaacctcc
    18961 tacccccagc ggattccgcg ccctcctgaa tccttggtgg tttgccgtca cccctgtgca
    19021 attcttgaac acccggcgga agaaccgccc cactcctatt tatccgcctt ttgaacccct
    19081 cggtgcccgg agtctccctg ttgtttcccc accacatcgg ggccgggcgc cttgggaggt
    19141 gcgccccaag aaccgtccta catatcccgt cagtaccaat gcttagcgac cggatcccct
    19201 tctctgcaag cgccggcaca accgttgcct gaaaccttcc cccaggtccg cagggggact
    19261 tcaggaaccg caattccaag gggaacgggg aacatggatg aaggcgtgaa agtccgggag
    19321 aaggattgga gcgttacccc gcaagaaggg atttcccggg aagtggagcg cgtgaacgcg
    19381 cgcctgaggc actttcgagg agttgccgcc ggcgtcatga aggacgctgt cgggatctgg
    19441 acggaaatct gggaactggt gcaggacccc cggtcctgcc gggagatcct cgagggagaa
    19501 gaacccgccc aaggtcgccc tccgtggggc gacaccggtc cgctcctcga aaaactgcat
    19561 cttctcggaa tccacctcga atacgcccga aagctttgtg aaggttccat cgggaacgaa
    19621 tctcaggacg aaaaggggca attatggcca agcagttgac cgtcatttcg ggaaacaaga
    19681 tcgggggaca atacccgctt cccgtccccg accaaagact caagaacttc cgcgccgtaa
    19741 tgggcgttgt cctggacgac gcccagaaga cctggggaga gatctggaag gaattccagg
    19801 gacaggtgac cgagggggtc ctggtgttgc ccgaggcgga acagggcttc aagcccccct
    19861 gcggatggcc ggagtttctc gagagaatgt ggctcctcaa gcactacctc gactacgcca
    19921 agaggttcgc cgacggggag gtgtgaaccg ccgaaaggag agcctgcggc gaacgtggga
    19981 ggatgcgggt cggaaaccgg aagaagtgcc tgcaatccgg gtcttccggg atgaggactc
    20041 atcggacatg acggctcatg ggacaggggt cccgcgggcc tgtgccaaac agaaaacccc
    20101 tagaccctaa gaccggaggt accccagaca tgtcaggagg aaacaatcac gacaatccgt
    20161 tcaagattgc ccagcaacag atccaggcct gcgccgacat cctcgacctg cctcagagca
    20221 cccgccaggt gttgctgcac cccatgaggg aattccacgt gaattttccc gtccgcatgg
    20281 atgatggggc catccgggtg ttcgagggtt tcagggtcca gtacaacgac gccaagggac
    20341 ccgccaaggg ggggattcgt tttcatcccg aggaaaccat cgacacggtg cgggccctgg
    20401 ccgcgtggat gacctggaag tgctccctgc tcgacctgcc cctgggcggc ggaaagggcg
    20461 gggtgatctg caaccccaaa gagttctccc ccgccgaact cgaacgggtg agtcgggcct
    20521 acatccgcgc catcggccct ttcatcggcc cggaaaaaga catcccggcc ccggacgttt
    20581 acaccaatcc ccagatcatg gcctggatgg tggatgagta ttcgtccatc gtcggaaaga
    20641 accagtttgg cgtgatcacg ggcaagcccc tggccctggg aggatctccc gggcgcggcg
    20701 acgccacggc aaggggagga atgtataccc ttcgggaagc ggcacgcaag gtcggtctgg
    20761 atttgagcaa ggccaccatg gccatccaag gctacggcaa cgcgggcagt tacgcagcgt
    20821 ctctcgccga atccatgttc gggatcaagg tcgttgcggc ttgcgattcc aagggggccg
    20881 tgacctgcaa ggacggtctg tgtgtgctca aggtggccga gcacaaggcg aacacctgtt
    20941 ccgtcctggg ctgcgaaggc accgtggatt tgagcaacga agagctcctt caaatggacg
    21001 tggacatcct cgtgctggcg gccctcgagg ggaccctcac ccagaagaac gcccagaagg
    21061 tgaaggcgaa gttgatcgtc gaactggcca acggccccac caccccggaa gccgacgaaa
    21121 tcctgtacaa caagggcatt cacgtgattc cggacttttt gtgcaatgcg gggggagtca
    21181 ccgtttccta tttcgagatg gtccagaacg cctacatgta ttactgggac gagaaggaga
    21241 tccacgagaa gctcgacaag aagatgaccc gggcctatca tgaggttttc gatacctcga
    21301 aacggcatgg gatcaacatg cgcaaggcgg cctacgtggt tgcggttcag cgcgtggtgg
    21361 acgccatgaa ggtgagaggc tgggtctagg aaagatttgg gagtctgcag ggaaggaggt
    21421 tgccgtgccg ttgccggaag cccgaatggt gagttgcggc atcccttccc tggacgaggt
    21481 catccagggg ttgcgactcg gggataatgt ggtctggcag gtggacgagc cggacaatta
    21541 tctccgggtc gtgcacccgt tcatgaaccg tgctgtcgag gacgggcgca ccgtcgtcca
    21601 tatccgcttt gcgcctcatc ccaccgtcct tcctccctcc cttcccgtgg cggacgagga
    21661 agtggacccg cggtacggct tcgacttctt cagcgcccag gtcaaccaga tcatcgaaga
    21721 gcggggcaga ggggtcttct acatcttcga caacctctcc tccctcgtgg cggactgggc
    21781 cacggacgaa ctcctggcca atttcttcca ggtgacctgc ccgtacctcc acgaactgga
    21841 caccatcgcc tacttcgccc tcacccgggg ccaacatgcc cacaccgccg tggctcgaat
    21901 ccgggacacc acccaggtgc tcatcgacgc ataccacgtc cggggcgacc tctacatcca
    21961 ccccctcaag gtctgggacc ggtactcgtc ccagatgttt ctgccccacc gctacgaaga
    22021 tgggctgctg catcccgtct tcaagagcgg agaagcctcg accatctctc tgaccgccag
    22081 caagaccccc ctcaggctca aggcggactc catcgccccc tgggacagcg tatatcgcaa
    22141 gctcacccag tacgacgagg aagagctgtc gctcctggag tccaccgccg agatgcaggc
    22201 cctcaagcaa gagctgtcgc agatgatcct gggcaacgac ccggaattca aacgtctctc
    22261 caacagatac ctcagcctga cggatctttt gtccgtgcgg aaccgcctca tcggctcggg
    22321 gcgcatcggc ggcaaggccg ccgggatgct cctggcccgc aacattctgc tcaagacccc
    22381 ggggggcctg gacttttccc aggtcctcga ggaacacgac tctttctaca tcggctccga
    22441 cgtcttcttc accttcctgg tcaacaacgg cctgttccgc ctgaggctcc gcctcacccg
    22501 ggattcccag atcctcaagg aggagttcga gcaggtggag aagcgcttcc tggcgggccg
    22561 gtttcccgac gagatcctgg agcaattccg ggacctcctg gattatttcg gccaggctcc
    22621 catcatcgtg cggtccagca gtcttctcga ggacggcttc ggcaattcct ttgccgggaa
    22681 atatcgaagc gagttctgcg ccaaccaggg gactcccgag gaacgaatgg aagccttcct
    22741 gcgggccgtg aagctcgtat acgccagttc cgtcaacccc gacgcccttt cctaccgcaa
    22801 gcaaaggggc ctcggggaag ccgacgagca gatggccatc ctcgtgcaac gggtgtcggg
    22861 gatgcgctac cggtcctatt tctttcccac cctggccggg gtggccttct cccacaacct
    22921 gtaccggtgg accgaacgga tcgatcccaa ccagggcatc atccggctgg ttttcggcct
    22981 gggcactcga tccgtgaacc gcctgggcaa cgattacccc cggttgatcg ccgtcagcca
    23041 tccccagctc cgccccgagg tcggttccaa gatcgccttc tattcccaga ggggcgtgga
    23101 cgtgctgaac ctggcccaaa acgaatttca gagcgtgccc ctggcggacc ttctggcccc
    23161 cgccgattac cccaacctgg gtctgtacct gtccggcatg gaggacggct acctgaagga
    23221 ttccatcgtc ccccggcagg aggacccgcg caaatgggtc ctgaccttca accatctcct
    23281 gtccaagacg gccttcatcc ccatcatgaa ggaactgctg gcgaccctgg aagccgccta
    23341 cgggtacccc atcgacacgg agttcacggc ggccccggat tcccggggca atctgaggat
    23401 caaccccgtc cagtgccgac ccatgaaggt gccgggggcc gtcgaggtcg tgggaacgcc
    23461 ccgggacttg ccccggagcc tcgtgctgtt ccggacccac cgggccatcc acggcggggt
    23521 ggtcaacggg attcgcacca tcgtgtatat cgatccgcga tcctactccc gggaaacccc
    23581 cctcgatctc aagcgggcca tgggacgcct catcggagag ctcaaccggc acccgcagat
    23641 gcgaacccgg cgcttcatga tgatgggtcc cggccgctgg gggagctcga acatcgccct
    23701 ggggatcaac accacctatt cggacatcaa tcacacgtcg gtcctggtgg agatcgcccg
    23761 ggaggaggcg ggccatgtgc ccgacgtatc ctacggcacc catttcttcc tggacctggt
    23821 ggaatccggg atcatttacc tgccccttta ccccaacgac cccgaggcgc acttcaacga
    23881 agacttcttc gccggggccc ccaacgctct cctggagctt ctcccggatg ccggggactt
    23941 cgaacgattc ctgaaagtca tcgacgttcc cgccgttacc gggggaaacc acgcccaggt
    24001 gatcgccgac ccccgcagcg ccgccgctct gtgctacctg gcaccggatc tttcgtcgtg
    24061 agcttgcgtg aaaggaaggg aggacgggcc tccatgagct ccgatcaaag gggattcgat
    24121 cgcagccggt cccgtcgacc ttgaaggtcc cgtcccaggg tctttgtgag cttcggccgg
    24181 gggtctattc gctccccgga gctcccaggt cgaatccgct ctgaaacgcc ttccaattgg
    24241 cctcgaggac cttgccggga agcacccgct ccatggcctt gcgccagctc ttcccggaca
    24301 cgggaatctt ccccgttccg gccagcactc ccaccatcac catgttggct ccccgttcgg
    24361 aaccggacct tcgagccgtt tggacggcat ccacccagat cacccgggcc accttcgcct
    24421 cgaggctgga tcgaatgagt tccatggacg gataggtgct ctgtcccacg gccacggtga
    24481 agggaacaat gggaacggtg ttggcgacga cgacggttcg gccgtggcac ttgggaagcg
    24541 cccgcagggc ctcgaggggc tcgaaagcca ggagaatgtc cgcctctccg tcggcaatgg
    24601 tcgggctcga aaggttcccc acggccacgg ccgactccac cacccctccc ctttgggcca
    24661 ttccgtggat ttcgctgacc accacgggaa gcccctcctc gagggcggct tcgcccacca
    24721 gccgggtggc caacaaggtg ccctgtcctc ccactcccgt aaaaaagatt ctcatagtcc
    24781 ccagacctca ctctcttccc ggtaggtttt cggttctcag ccctcgatct tttccggctt
    24841 gatggacggg cacatctggg cgcaaaaggc gcacccgttg cacagggcct cgttgatccg
    24901 gatgcattcc tcccccgccg ccgaggcttc ccagacgaag gccgggcacc ccagttcccg
    24961 gaggcatcgt ttgcaccggt cgcagtctcc ctgcaccttg aagacgatct tctgtttctt
    25021 tcccgtcatg cggcgttcga acagggggca ggggctcttg gcaatgagga cggaaacgcc
    25081 cgggtccttc atctgccgcc gcaggtcatc gaggacctcc tgggtttttt tcatctggag
    25141 cgggttcacc agggccacct ttttcactcc gcacccgcga accacgtcct cgatgcgcac
    25201 ggcgggctcg atccttcccc ggcgggtcca ttccaccccg gggtgaggct gatgtcccgt
    25261 catggcggtg gtcccgttgt ccaggatcac caaaaggaac cggtggtcgt gggatacggc
    25321 gttgatgagg ccggggatcc ccccgtgaaa aaaggtcgaa tccccgatga aggccaccac
    25381 cggacggtcc agcaccctgc tgaagccccc cgccgtggaa acgctcgaac ccatgcagac
    25441 aagaaaatcg gccatgcgca ggggaggaag cacccccagg gtataacacc cgatgtccgt
    25501 ggggtagacg gcttcatccc cgaaaacctt cctcacggaa aaataggtgg cccggtgggg
    25561 acacccgggg cagagggtgg gagggcgagc cgggagctcc ggggccgaaa agatcgtcgg
    25621 tgggtcgtag gggacctgga aacatcggcc gacggctcnn nnnnnnnnnn nnnnnnnnnn
    25681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25741 nnnnnnnnnn nnnnnnnngc cgaaaagatc gtcggtgggt cgtaggggac ctggaaacat
    25801 cggccgacgg ctcgtttcac cttcacggaa tcgagctcga aggcccgggg aatccactgt
    25861 tcgcccttgc cgacgatctt caccgggatc tcttcttcct gggcaatccc cttgacggct
    25921 tcttccagga agggctcgag ttcctccacc acgaggactt tcccgactcc tcggaggaag
    25981 tttcccacca gcttccgggg catgggatgg gtgaacccga gcttgaggac cttcaccttg
    26041 ccttccaaac ccaggtccct caaggcatcc tgcacgtaga gccaggagac tccgctggtg
    26101 atgatcccgt tttccccttg tcccgtgacg aaattgaagg ggctcttctc cgcttcctcc
    26161 cgcagcattt tctccttctc catcagggcc aggcgaagct tgcgccccac catgggaacc
    26221 acgaccaggt tgaaggggtc cttggtgaaa gacgcggtcg gtttttcccg gggaagctcc
    26281 ccgaaacgca ccggtccccg ggaatgattc acccgggtgg tggtgcgcag catgcacgga
    26341 atccggtagg cttccgaaag atccagcccc gaaagggtca tttcccgggc ctcctcgggc
    26401 gtggagggct cgagcatggg aacgcccgcc atcttggcgt aggcacggtt gtcctgctcg
    26461 ttctggctgg aatggaggga cggatcgtcg gccaccacca tgaccatccc ggcgcgcacc
    26521 ccgacgtagg ccagggtcat gaaggcgtcc gccgccacat tgagccccac gtgcttcatg
    26581 gaacacaggc ttcgaacacc gctgagagcc gctgcacagg cggtttccat ggagaccttc
    26641 tcgttgaccg agtattccac gtacaccccg gcttcctcgg ccaactggga aaatcgatcg
    26701 atgatttccg aggaaggagt ccccgggtag gccgccacaa agcgcacgtt tccttccatc
    26761 gccccgcgga cgacggcttc attgcccagc agcaaatgtt tccggttgct tgcgggtacc
    26821 aacaactcat ccatcctgat cctccgttcc cgatgcctgc tcccgccggt ccacgaaaag
    26881 ccggacctct cccccgaccc tctccaggga cccccgctcc accagcttga tgcggggagt
    26941 gatcccaatg aaattggtca ccttccggcg catgacgtcc accagagttt tctgatcgcg
    27001 cattttgtcg tagaaaagct ggtcggtgac ctccacccgg atttccagct gatcctggtg
    27061 accccggcgg gttgccgcca gttgatagac cgggcgttct cccttgatct cctcgagtat
    27121 gtctccgatc cgctggggaa tgacgttgat tcccttgatg accaccacat cgtccgagcg
    27181 ccctcgaatc cggccgagcc ggtggaacgt tcgaccgcag gggcacgggt ccgggtggat
    27241 gctgcacagg tccccggttc ggtatcgaat gacgggaaag gcttccttgg cgagggtcgt
    27301 gatgaccagc tcgccttccc ggccgggttc caggacctgc cccgtttcgg ggtcgatgat
    27361 ttccgcaagg aaatgatcct cctggaggtg catcccttgc tgttcgaggc attctcccgc
    27421 cactcccggc cccatcacct cgctgatccc gtaattgtcc gtggccttga caaagagcct
    27481 ttcctcgatc tcttttcgca tggattcggt ccacggctct ccggcacaga tgcagtagcg
    27541 cagggacaac cgcttgggat ccatccccac ggcttcgacc ttgtcggcga tgaccagggc
    27601 gtaggacggg gtggacacca agaccgtggt gcgaaagtcc tgcatgatct tgatctgccg
    27661 ctcggtgcgg cccacggagg tggggagcac cgaggccccg atgcgctcgg ctccgtagtg
    27721 aatgcccagt cccccggtca ggaggccgta gccgaaggtc acctggatga cgtcgtcctg
    27781 ggttaccccc gcggcactga ggatcctggc caccaggttg ctccagacct tcaggtcctg
    27841 gcgggtgtag cccaccaccc ggggattcac catgaccccc gaggacgaat gcacccggac
    27901 cacctcccgc agcgggaccg caaacatctc gtagggatag ctgtcgctca cgtcccgggc
    27961 cgtggtgaac ggcagccgac ggatgtcccg caggtcggtg agatcgtctt cggggcgaaa
    28021 gtccatctcc ttgaagcgac gtcggtagaa ggagacgttt cgatagaccc ggttgagagt
    28081 cgcctgcagc cgttccagtt ggagctgctg cagctcgtcc cgggacatgc attcctcttg
    28141 cggttcccaa atgggcatgg cggcatccct tcttcaaaga ccctgaacac ggagacgggc
    28201 tacgcttccc agatctccct tgcccccttc cccaggtagg cccggcgcac ctccctgtgt
    28261 tccaggagca tggaaccgtc cccctcgagg accatctttc cggtttccat gacatagccg
    28321 cgatcggcga tctggagcgc ggcccgggcg ttctgctcga tgagaaggat ggtggagccc
    28381 tcgtcgcgca gttcccgaat gatgtggaag atttctttga cgatgagagg agccagcccc
    28441 atggaaggct cgtccaggac caccaaacgc ggtcgggaca tgagggcccg gctgatggcg
    28501 agcatctgct gctccccgcc gctcagggtc ccggcaagct ggttcctgcg ctgctcgaga
    28561 atggggaaac gccgaaacat ccggtccatg tcctcggcca cctgtcggcc gtttcgacgg
    28621 tggatgaagc ctcccatctc gaggttggcc tggacgctca aggtcgagaa aatctgccgc
    28681 ccttcgggca ccagggcaat ccccctgcgg acgatttcct cgggaggaag ctcccccacc
    28741 gagtccccca ggaactcgat gcggcccttg cgacacggga cgagtccgca gatggtgttc
    28801 accagggtgc tcttgccggc cccgttggca ccgatgaggg tcacgatctc tcccgggcgc
    28861 acgtgaaggc tgaccccttt gagggcgtga atgcttccgt aataggtatg aacggtcctg
    28921 accttgagca tgacccgcgg cctttcccct tcacgattct cccaggtacg cctcgatcac
    28981 ccggggatgg tccttgattt cccggggaga tcccgtggcc agggtttccc cgtaatggag
    29041 caccaggatc cgatcggaga tgtccatgac caggttcatg tcgtgctcga ccagcatgag
    29101 ggtcgttccc tggtcgcaga tccggcgaat gagagtcccc agctcttcgg tctcccgcat
    29161 attgagtccc cccgccggtt cgtccaggag cagcagttgc ggctccgtgg ccagggcccg
    29221 ggcgatctcg aggatctttt gcttgcccag ggggagggct cccgccgcca ggtcggcatc
    29281 cagacccagg ccgacgaaat ccaggtactc gagggaccgc tcccggatgt ttctctcctc
    29341 ccggcgcatc ccgggaagtc gcagccccgt tgcgagaaat ccccgggaag aacgcgggtg
    29401 ccggcccagc atcacgtttt ccagcacggt catctccttg aacaaggcta cgtgctggaa
    29461 ggtgcgcgag atccccattc gtgcgattcc atggggcggc aggtcgtcga ttcttcgacc
    29521 tcggaaaaaa atctccccgt ccgtggggcg cagcagaccg gtgatgcaat ggaaaagcgt
    29581 ggtctttccc gccccgttgg gtccgatcac cgcctggacg atgcccgggg gcacccggta
    29641 ccgaatgccc ttgagggccg ccacaccccc gaagttcatg cgcacttccg ccagttcgag
    29701 gatggaggaa tcgccgttgg ggcttttcat gggtttccgt cctttgggga ggacttctcg
    29761 gcagacgatg ggaaaagcct ccggggaatg agggaaacca ggcccttggg caggaagagg
    29821 gccaccacca ggagcaggga gccgtagacg accatttcaa actcggcaaa cacgtggagc
    29881 cattcgtttc ccacgaaggt gaggagaacg gttcccagga gcgtccccca caggctttgc
    29941 atccctccca ccatgaccat gagcatgaac cgcatggacc acatgaggtt gaaggacccg
    30001 ggattcaggc aggtgacgta gtggttgtag aaactcccgg ccacggacgc caggacggcg
    30061 ctgatgacga aaacctgcag tttcaaccgg gccgtgggca cacccatggc ctcggcggcg
    30121 acttcctcct cgtggatggc ccgtagggcg cgaccgatcc gggaatggat cacgtggaag
    30181 cagaagatca gggtcagcaa aaccacgccc caaacgaggt agtaccaggc aatctcactg
    30241 ttcagcttcc agccgaagat cttgagcccg ggaatgtcca ccatccccga cggccctccc
    30301 gtccattcca cctcctcggc caggatgata tgaacgatga cgccgaatcc cagggtggcc
    30361 atggccaggt aatgtccctt gagacgcagg gccgggatgc ccaccagcca ggcaacggcg
    30421 gcggccagtc ccgcccccgc gggaatggcg gcccaggggt cccagcccaa acgggaggtc
    30481 aggatcccgg acgtgtaggc cccgatgccg aaaaaggccg cctgggccag ggagatctgc
    30541 cccgcgtacc ccataagaag gctcaatccc agggtcacta ggctgaaaat cccggcaaac
    30601 accatgacgt ccacgtagtg ggaaacggcc ttcagcggct ggacaagcca cggaaaggcc
    30661 gcaaggcaga ggatggttcc caacaggatc cggaggtctt ttcgctgcac cggggcgatc
    30721 ctctagaatc tcttgagctt cgccgcttcg cggctgccga agagaccctc gggcttgaag
    30781 aaaagggcca gcagcaggat caccagggcc acggcgtcct tgtagcccga ggacaggtat
    30841 cccgcacaaa aggattcggc caggcccaac aacaccccgg ccaccagggc gccgaaaaac
    30901 tgtcccaacc cgcccagggc cgccgctccg aaccctttca gacccagcag ggccccccgg
    30961 tcatattcca tgagggagat cggcgtgatg atcacccccg ccgcagcccc gatggaagcg
    31021 ctcaggatga aggagagcag caccattcgg cgcacgggaa ttcccaccat gcgagccgcc
    31081 cgggggttgt cggcgcaggc cagcatggct ttgccgtaaa cggtcctgcg gaaaaagatc
    31141 gtgagagccc ccaccaccaa tccgctgatg gcaagaatcc ataaggcctg gggctggacg
    31201 gcggcccccg ccgccatgat gggcccgccc ctgctgaagg cgggaagact gtaggggtcc
    31261 tttccccacc ccagcatggc gccccccttg aggagaatgg acagggcaat ggtgatgatg
    31321 atgagggaaa ggacgctggc gcttcgggca cgccggatca cgaaacgttc catggcgaac
    31381 cccaggatcc cggccccggc cacggcggcc gcgaaggcca gggggatggg aaatcccagg
    31441 gatcccacaa aaaagacggc caacaggccg cccagcatca cgaattcccc ttgagcgaaa
    31501 ttgatgattt cggtcacgtt gtagacaatg ttgtacccga tggccaccag ggcgtagatg
    31561 accccgatgg tcaccccgct gatggtgtat tgcaggagat ccgttgtgat catgcttcag
    31621 gccgcgcaat cgttcgccgc gagggtcgca gggagcaggg gcaatgcagg gaatctcggc
    31681 gtcccgggga tctcggcggt gattttccac ctttcacggc ccgggggcct tccccgggac
    31741 cacggcaggc ggggtccaat gatttctcgg gccgcgcccg gcgcggcctc cttccctcga
    31801 tgaggcgagg gcctactttt atgaccggaa acgggggaaa aatcaattcc cattccgcct
    31861 atccagcatc gagcccatcg cctccagcct ccctgccc
//
LOCUS       DAOOVC010000031        31331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_966_length_31331_cov_6.901874, whole genome shotgun sequence.
ACCESSION   DAOOVC010000031 DAOOVC010000000
VERSION     DAOOVC010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 31331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31331
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_31331_cov_6.901874"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLM79_06250"
     CDS             <1..751
                     /locus_tag="PLM79_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOV85945.1"
                     /translation="GISRSGYYYEPAMESPFNLMLMRLIDEHYTRFPFYGSPRMTAWL
                     RSEGYGVNHKRVERLMRQMGIQGVCPRRNLSKGCDGHRKYPYLLAGLTVDHPNQVWCS
                     DITYIRLVKGFVYLVAIMDWYSRFVLSWEISNTLDTHFCMESLERALLRGTPEIFNTD
                     QGVQFTSAAFTSRLLDEQIRISMDGRGRAFDNIFIERLWRSVKYEEVYLKDYETVMDA
                     VTGIGSYLDLYNHERLHPSLGYRTPAEVYGS"
     gene            1365..1559
                     /locus_tag="PLM79_06255"
     CDS             1365..1559
                     /locus_tag="PLM79_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85946.1"
                     /translation="MVSYIVRIYRRADALEGRAAGIAVDPETGEEVGFSNAQELWYIV
                     NRDRSAAPVTAAEEKEEEKR"
     gene            1746..2963
                     /locus_tag="PLM79_06260"
     CDS             1746..2963
                     /locus_tag="PLM79_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85947.1"
                     /translation="MGKREALGLTLATEEVFAHLCAVVLKDGEKVEIHCTNGGYYVQV
                     DFTFPASDMDLRAFNLNATVSFADDADLEEMGLVLASRSVDRFAVTRLEGKGLKLTLL
                     KEKAYPPFDESPVAIPRPMDRFSVRPPNPEELKLFAHLVKTCYGNQVLPKVFDHPGKL
                     VDMLAGANYGAAVAVGPAGGTGGGTFWHRAGEKAVECFGPYLFNQKAGSPIPGALLES
                     CVGAIARTSALVLINRFATPEFAREHFEPLGSVRMHTSEGELSRREAWFRLMHEDPGS
                     IAWVHPELETFLQQEYRRLVLPRELRQVTNAGEQHPRHSVISTTFDRPQASATLRPIW
                     FGTDFASNLVQHLKLLRQEGILNIFFSLDVGKSWQAAFTPGLLQNGFTPCLIFPCSGE
                     GDMVLFQFQGATP"
     gene            2960..4090
                     /gene="hisC"
                     /locus_tag="PLM79_06265"
     CDS             2960..4090
                     /gene="hisC"
                     /locus_tag="PLM79_06265"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOV85948.1"
                     /translation="MKNLPPLDRLVPPHIRQFQAYIPSKPDGELKKMYRCPRLYRLNN
                     NENFLGPPPAALEAIRAFPSPQAAVYPSGDAYYLRCRLADRFGMHPDQFLVGNGANEV
                     ISFVIKAFCREGDNIITADKTFAVYEWVAEFSGFEARLVPLVNFGFDDEGMLKRIDER
                     TKMLFVCNPNNPTGSIWTTERLCRFLDRIGGRQIVVVDEAYAEFVEDPAYPDGMALIR
                     EYPNLVVFRTFSKMYALAGLRIGYLAGSKEVVDVMRRTCIVYSVNSLAQAAAVAALED
                     SGDHSAKTRELIRQGKAFLKKEIEATGLPYVLGEGNFMMIKLPMNDTLAYRKLMTHGV
                     MVRSMTCFRFPNWIRVTISHQEAMEAFVEALGKVLAGGAVRK"
     gene            4160..7207
                     /locus_tag="PLM79_06270"
     CDS             4160..7207
                     /locus_tag="PLM79_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV85949.1"
                     /translation="MSEILLNAILHLFAIQASLLDHLSRARARAVVERYLETNLRIAD
                     PGPYLDLYDMLLEFQEDRDGRARLEQAREIARKLKPVLSRFDQYAFLLHYLTLAAKVG
                     AAGAAMGAAEAVALELAIPWERLDDLRIFSESPFDPARLTESFLVHDPEDCLGQMQCR
                     RIVRPDFKGGFTVLRLSDVETCFILPAEDSRIILDSSPMESGTLHIVRPGAILRDAFG
                     TQIYHAEIEAAFNRSATGVGVLFCGEDLNFRYPGSDNGLHNFSFSVKGGGMIGIMGVS
                     GSGKTTLLNILTGRSRPQSGRVLIDGIDLHIEPERLEGVIGYVPQDDLLFEDLSVFEN
                     LYYNASLCLANLDPAERADRVDALLDELHQLSIRNLKVGSPTEKSISGGQRKRLNIAL
                     ELIREPSILFVDEPTSGLSSSDSENVMALLKAQAAKGKLVIVVIHQPSSRIFKLFDTL
                     WILDQGGRPIFDGNPLDSIVYFRTSVHQAGMGEYACPRCGNVNPEQLFEIIEAKAVDE
                     NGYYTRQRLISPGEWHQRYLDHRRGHAGSNGGEAESSSNTEKRLWRPGRLGQVCVFFM
                     RTLKSRLSNHQYVFINLVEPVVLALLAAFITHGARGGEYVFMENGSLATYFFISVVVA
                     LFLGLSVSAEEINRDRSILQRESFLHLSWPCYIMAKSLYLMLVTAIQMALYVSFGNGV
                     LEIPDMFPTMFLVLFSCGAVSCVLGLNISAALKSAVAIYILIPVLLVPQIMLGGAVVP
                     LEDLIHRQAGNRYTPLAADCMPSRWGLEALIVKQYVSNRYMRNFFEDDCSIKQCDYML
                     SFHLPEMSALAAYPFLETDVPDKPSQIARKLTTLGNEIRLLEQSTGLSSGIQADVFTP
                     SGFTPEVERLARGYLETVAEKYRAARKASLKQKQETEDALRSRLGRKGLSALKEAHYN
                     ESIADIALRTKVLDAIRLSGGRLVQTAAPICQTPESNWGKAHFPAARKRIGSWLIPAH
                     VFNVVVLWAMTALLYLALYFSVLPAILSSAESAANRLRRRKRP"
     gene            7410..8261
                     /locus_tag="PLM79_06275"
     CDS             7410..8261
                     /locus_tag="PLM79_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85950.1"
                     /translation="MNGAIAGNVVKIVVVVLLTSAFWSFSHAGEGDLKAAAKVLADGV
                     QPTKEISDHLELYKKAGITYQPDLPVPLKDIADCKSQEQLRVLMGMYAFELGYAMAFE
                     KRKELLETSRAIQSEIVDRLEIRDKLDVSLAKAEPLGSDKEEADGRGRDELLENQKNW
                     IMNMIEKAENDPQVMELLMDIMYGGSIEGLHVICNIALNAEKGDALIALLNQRASRLE
                     IMDAALKCFNDKDLASKMKKDSRVKFSRAVIDLVKAKKGALTREDMQQILALVEPERN
                     IYVKKCE"
     gene            8626..8874
                     /locus_tag="PLM79_06280"
     CDS             8626..8874
                     /locus_tag="PLM79_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85951.1"
                     /translation="MLECCDRVCTPRQIEEYCARCGHHHFGAVSKAIENLLKRKVLLH
                     QDGSYLSLAAHPPRRAYVPLGDFPGGYYYPESGPPVCD"
     gene            8865..9050
                     /locus_tag="PLM79_06285"
     CDS             8865..9050
                     /locus_tag="PLM79_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85952.1"
                     /translation="MRLKNRLTEFLARFLLIVVTSSTDMLRSMTCFRFPDWIRVTISR
                     QEAMEAFVEALAGIIRE"
     gene            9440..10243
                     /locus_tag="PLM79_06290"
     CDS             9440..10243
                     /locus_tag="PLM79_06290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOV85953.1"
                     /translation="MGESHLFGVKLFHERDLWSFRLKGLLDERADSDWEILNFSFPGY
                     NTHQYKAFFEHELLGLKPDLLVLSIGANDITQARIFGEKWHPGMVYPLDFICALARKS
                     PWWEKLLNRSCLCSSWLRKAREAQRSAFFKTGASFGKEECFQAAFTNIELIARSAQAC
                     GVRVIFMLLCPVYELLMSAEDQRKITSLLSDWRRYVDGDGPAMFEFMERMNSEVAPRL
                     DMPVIDLRPRFWRHPNRFKLYFDLYHLNPNGMRLLAESLYEEIHKLGCW"
     gene            10345..11553
                     /locus_tag="PLM79_06295"
     CDS             10345..11553
                     /locus_tag="PLM79_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85954.1"
                     /translation="MEEPKRLYTFEFISLCFVSFFGFCNMSVFYSFFSYLGRIGIPEE
                     WRGFLVGLEPMSAFALKLAIIPLLHMGNAARAMLIALLMTIVALCSYTLAVTVPALII
                     LRIFHGAAFVLLVSAAMAFAAHLIPRERSAQGFGILSITATLPYAVMPLVTEALLPYV
                     KNESTIYAGVSVLAVPGIIVLCVLRKRMGALFAGVHASLARRPSLDELRRNLKLPAVV
                     LILAVSLILYFGNATLFFFVKGYFKEIGTANAGHFFTASTLVMIAVRALGGRLMDKAD
                     KVKTMAFFTFLLVPWFMAFGYLRSPMPFILLAACFGLCAGIVFPMLSSAMLQASPAQL
                     RGLNTNLALFMMDTSFFLSPYAAGMLFAGGGSFEDLFHICSGLLALAVCLLVALGRLD
                     WQSKYWQCTP"
     gene            11583..12665
                     /locus_tag="PLM79_06300"
     CDS             11583..12665
                     /locus_tag="PLM79_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85955.1"
                     /translation="MNKQEAIRKLREEAYQVKPDQEFEVRRMEPEDAWGVVRCNFAVY
                     GEHYPIDTNYIPERLIEENRLRNLYTVVARTGNGDIIGCGALYRSSAYSPRVYEYGQA
                     IVHPEYRHTFAVLCIQDYILNVLVSQEDIDEVFGEAVCNHLVTQKLAALADFRETGLE
                     VGVMSAEAYPSEDFPGERVSTVLHFRSVRDRMQEVYVPRQYEQVLEYIFSGLEISRSV
                     LPSEARAPSGSATGIKTQIFDFAQVARFNVYSLGEDFPAVLAAEEEKARSRGTEVMQM
                     FINLGEACAGRAVDYLRDKGYFFGGFLPRWFDTDGLFMQKLVKLPNFGSIKLDSERAH
                     KILGLIRRDIEGNPACRALLSRSCWR"
     gene            12711..16013
                     /locus_tag="PLM79_06305"
     CDS             12711..16013
                     /locus_tag="PLM79_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV85956.1"
                     /translation="MLTARILDEFESRLTRSGEVSFHSSGAGHESTAALAFHLGPSDW
                     LHCHYRDTALMIARGVSPEAFFSSLLCKDTSHSRGRQLNVMMSCPELNLLSIVTPVGN
                     GALQSVGVASAIRDDKENPIVLCGLGDGMTQQGEVCEAIGEAARDSCPVLFLIEDNQY
                     ALSTRTRGRTFYDMGGERAASFYGVPILYCSGSEAVECYGIFGNIVSEIRRTRKPRIL
                     VLHVPRLSDHSNADRQAAYRSPDELLEAQALDPIPRLEKHLLRSGISEAELRLIRVEA
                     EERIQKAADAARNAPDPQPCFSAKAPLPDCLSRQSFPEPLYGSRDGLTMVEAIREVLR
                     SHLEKNDKVTLFGEDIEDPKGDVFGVTKGLSTQFPGRVRNSPLSESTIVGKAIGQALA
                     GGRPVAFLQFADFIPLAFNQIMNELGSMYWRTDGGWQAPVIVMIACGGYRPGLGPFHA
                     QTLESIAAHVPGIDVMMPSNAADAAGLLNAAFKSNRPTLFFYPKSLLNDRQSTLDADA
                     GEVIVPIGRARITREGKDLTFVGWGNTVRLCEQVAETLSEHGFSAEIVDLRTLSPLDE
                     ETIQRSAEKTGRIIAVHEDNRTCGLAAEVLACVSERLKTHVRMTRVTRPDTYVPCNYA
                     NQLSILPSCESILEAAAELLDFSVEWRTEEEEDASLVFVKALALSPADEFCIVVELYV
                     KAGDKVKEGDVIAAVETNKAVAELASPASGVIEDVFVEEGQTVAVDERILSIRVKPDD
                     GAIRRKEPATRRSAVLSKRTSEMSEKTTSQISTAVPARATPVYISGVASSLGSRTIHN
                     ETLLKSFPDWSTQDVIKRIGIESRKWIGEGESALTLALAACTSLFEKLPVGFEDISAI
                     VCSTGTPISASPSMACEILTALAGNRSDKLVQAFDINAACSGYLYGLQVAHDMIQSSL
                     QSHVLLVTTEVLSPLLNLQDQSTVFIFADAATATLISSSPLAAKSARVKRPVLSAKGD
                     VARTLSIPFIGGAAAFITMEGVEVAIEAGKGMSFILTEACSRVGLTPNDLDLVIPHQA
                     NKRILAAVQRRLNLPEGKVYTNLKDIGNTSSSTIPICLDHVLPRMEPESRIGLVAFGA
                     GFTFGAAILETV"
     gene            16065..16328
                     /locus_tag="PLM79_06310"
     CDS             16065..16328
                     /locus_tag="PLM79_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85957.1"
                     /translation="MSEDEIVLKFSEIVLNVLGQPFELLGLNENLRESRGADSMDMVD
                     ILEATELAFGIDAGDGSYEKMVNVGQAVSLIKKLIESSRQSGR"
     gene            complement(16297..17592)
                     /locus_tag="PLM79_06315"
     CDS             complement(16297..17592)
                     /locus_tag="PLM79_06315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOV85958.1"
                     /translation="MAEKRRIVITGMSINTPLGDTLDEFLQNLLDGRSAITHWKLLDS
                     SRISSKIGGELYAYDVSGKISSLREQIPDETASRLPRLIRNSPWSLQLSVLVAVAAFL
                     DADLFTPVLNPERLAAIIAGHNLNEQYYHQNWERFVEYPDYSDALLALKGVDTDHAGC
                     VAEILGIHGPIYTVGGACASGNVALRSAMDEIRHHEVDIALVVGAVYEFSPVTLHSFA
                     RLGAISCTSFNDEPARASRPFDVGREGFVASHGSGALVVEELGHALARGARIYGEILG
                     AELRSAASRSPYPSEEHEALVMEQVVRKAGIDRQEIDFVSAHATSTVVGDVTEIRAIK
                     RAFGDHAGKLKINAPKSMLGHTLESAAVVETVAAVLQMRAGRLHPSINIDRLDPEIDL
                     DVCANGSVEHPVRCLLNNSFGFGGINSASIIGRFDGSTL"
     gene            complement(17585..18124)
                     /locus_tag="PLM79_06320"
     CDS             complement(17585..18124)
                     /locus_tag="PLM79_06320"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HOV85959.1"
                     /translation="MPLYEDKDQFLRQASRQALWRRGRATRLVSLGRAEIERLLPHRD
                     PFLLLDAITAIDYEQEAIEGIRRIDPEDPVLAGHFPGKPIYPGVLLIEMMGQLGVCMI
                     GLQSRKDKDEDFTDPAPPDVRLFRIHASVFFAPVMPGDEVTLRALALESNGLTVVFAG
                     QVSRGETHCAAAVMEGYFG"
     gene            complement(18242..18496)
                     /locus_tag="PLM79_06325"
     CDS             complement(18242..18496)
                     /locus_tag="PLM79_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOV85960.1"
                     /translation="MTRDDVLAMVIRNVKEVVPTFQGTETDLNKSYRDLGVKSLDLVE
                     IMTGIIRDMQMKIPIAEFAKISTINGLVDYLFKVKSELQA"
     gene            complement(18718..19308)
                     /locus_tag="PLM79_06330"
     CDS             complement(18718..19308)
                     /locus_tag="PLM79_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOV85961.1"
                     /translation="MILTAWRLVEAKYQDAAFSGEGASLYGGRWNSKGVPVVYTADSL
                     ALASLEIIVNLPSPKLLGAFVRIPVCFTPDLVESIPISSLPGDWQSRPVSPATRALGD
                     QWVKDQRSAVLKVPSIVVPEEFNYLLNPAHPDFGKIEIGPPVVYYFDPRLAKDTGRAT
                     SRSKRLPERARRYSASARTSLMGQTVLRTRAAACRA"
     gene            complement(19305..19706)
                     /locus_tag="PLM79_06335"
     CDS             complement(19305..19706)
                     /locus_tag="PLM79_06335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOV85962.1"
                     /translation="MRATSIGLEPENLTQLIARIKKGLPTELFEQLCRNLTIPEKELC
                     RILGIPLSTLARRKKSGQLSFEESERVFRIARLYDRAVEVFKQPELGRKWLKEPAWAL
                     GDVPPLEFAATELGAKEVEDLLGRIEHGIFT"
     gene            19690..20217
                     /locus_tag="PLM79_06340"
     CDS             19690..20217
                     /locus_tag="PLM79_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85963.1"
                     /translation="MDVARIITSPPVIVILKYGNFHMGICKAESKDGILFLLRERSAA
                     GCLRPGLFLTRSGRFVRFVLPSPQGPDYCATICRKKPGRVPPGAMRREPGSGNLFQVI
                     TRRLTMQGFIVMDHMDKRARFLADMGNWIRQGRIKWNEMIVGGIENAPKAFIGLFKGE
                     DLRKMLVKIGPDPAT"
     gene            20234..22981
                     /locus_tag="PLM79_06345"
     CDS             20234..22981
                     /locus_tag="PLM79_06345"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV85964.1"
                     /translation="MSAEPENPWKEWLLEVVWQLQPLAEPVREPWVPRPSLTEIHDLL
                     LPEAAILMAQPGIAAYEQTLAKLEKAGIAYVLKALEGAGWKFNSGKRFSTIEMALQVG
                     VVDPYHRLLERLLEMLSEEGILQRLDGAWEVVSEPEPGDPEAYMLGLSCPGAEAEIAL
                     LERCGPELARVLQGRSYPLELLFPEGDTALLAGFYEHSPVPEMMNGLAQRAVLSILDR
                     LPRDHCRRILEIGAGTGSTTARLLPHLPDDRTEYVFTDVSHLFLNGARKRFQEHPFVS
                     YRLLDIERDPQSQGFEPGRFDLVLASNVLHATRDLRESLQHVRQLLASGGVLMLLETT
                     GAVRWLDLVFGLTEGWWRFSDHDLRPSSPHLSAGQWKTLLDSHGFEDTVSISPDDPPK
                     YGLIPAGRNLLPESLILARKTAGLEEVHRPERWLVLADAQGIGRELRDRFHSRKDDCV
                     LVFRGGEYEALSQQEYRIDPAEPEHFRRLFHAVQGELDGVVHLWSLDVPLLSAATAPH
                     LDDTGHTLDRALLLGCAATLSLIQAMTDAYIGPPSLWLVTRGAQPAGRECTVPGLFQS
                     PLWGMGKVIPLEHPDLSCVRIDLDPEGAEPVDHLFRELNRKEAREDQVAFRNGQRYVA
                     RLSLHNAVIGSSPCSEPASGSRIPLTFSADGSYMVTGGLGGLGLLTARRLVERGARNV
                     ILVGRNPPGPEAAGRITEMERRGARVTVIKADISDAKQVAEILDDIEAALPPLRGIIH
                     AAGLLDDGILVNLTWERFSRVMAPKVNGAWALHALTASRNVSLDFFVLFSSAAAMLGN
                     AGQANHAAANAFLDALAHHRRAQGLAGLSINWGAWSETGIVAGDQRAAKAMKNAGLGG
                     ITSREGLIILEQLMACSPAQIGVSPMDWPRFIKRFQLQGTAFFDHFPDPGGKSPQRQT
                     Q"
     gene            23001..24302
                     /locus_tag="PLM79_06350"
     CDS             23001..24302
                     /locus_tag="PLM79_06350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOV85965.1"
                     /translation="MSQSRIAITGMAVNTPLGDKLDEFLNNLLQGRSAITRWKFPDSG
                     KIYSKVGGDLSDYDIAGKVSSLRELIPEAVAQRLDKLVSYSPWSTKLSVLLAADAFLD
                     AGLFTGLADPERIAAIIAGHNLNGMYMYNSCREFGEEPDFIDRMLAVSCHDSDHAGCV
                     ATGLDIRGPVYTVGGACASGNIALRSAMDEIRHHGMNVALVVGAVYDCCPLVLHSQAM
                     MGAVSASSFNDRPTMASRPFDLAREGFVPSHGGGALVVEAWEHALERGARIYGELVGV
                     EVSCAATRGPRPSEEHEARVMEQVLRSTGTDPHQVDFVSAHATSTQLGDLAEIGAIRR
                     VFGDHARALKINAPKSMLGHTLMPSALVEAVAAVLQMRAGRLHPSINIDRLDPAAMDL
                     DVCANRVVEHAVRYLMKPAFGVGGFNSASIIRCYDEMIHWR"
     gene            24367..24621
                     /locus_tag="PLM79_06355"
     CDS             24367..24621
                     /locus_tag="PLM79_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOV85966.1"
                     /translation="MTRDDVMAMLIRHTCEIVPELRKEHLDPDKSYREMGIDSLALMA
                     ILTAATRELKVKIPRDKLGNINSLNGLADLFVQVANQPEA"
     gene            complement(24661..25134)
                     /locus_tag="PLM79_06360"
     CDS             complement(24661..25134)
                     /locus_tag="PLM79_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85967.1"
                     /translation="MLKTLRIIHLGFILSGMNLSRNVVASGTRIPSGLSTQLQTQFFD
                     SARVARFGASCMGENFPEMLATAEKQAQSREIQVVQVYVNLGEAWSGWAASVLREKGY
                     FFGGFLPRWFDTDGMLMQKLVTLPKFDSIRLFSRRARDILEFIRRDIKKHSYGKS"
     gene            25172..27955
                     /locus_tag="PLM79_06365"
     CDS             25172..27955
                     /locus_tag="PLM79_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOV85968.1"
                     /translation="MISRSEPEAEFARIASYGRMHHVGWDGLRFTLEETKQLARQFAR
                     AEMSDTRIEQIHRKTDGWVAGLIMAMEAARNGNLSEAFMERLSGESFFDYFTAEIFER
                     LDPSIRSFLLATAFLPEVTIGSSERLTGSRNAGRILSDLHRNNFFIWQSDSEPRVYTY
                     HPLFQDFLRTYAGRTLEAETLDRIRARAAQIMEEQGRIEEAIDLFIEQKDWKSALRLL
                     LTHARAALIHGRNGALEKRLARLPAAVLESTPYLSYWQVMCRLPEDSRESRYHFEDAF
                     RRFETEGDTVGALLAWSGVVETLILELGDMRRLDPWIQWFDDRSGDEALFSSPEIRMM
                     AARSMLGVIALRRPDHPRAEYWVSEAEALLDSGLEMNSCVLLAARLYYYYSLMEENSR
                     TNLIFKRLEPLIDPNRLQLMTRLWWQFLVLHHSRYAAWQTRESCEAYFEKVHQAMSLT
                     RDLGFRTFDRFNPSFGAHVALVARDFKTFDEMVGVLRESLDDSNILGHILYQVYLGLK
                     FVYQGDYASAREQGKIALRLAENMGSPLYEYRGHILLFHSYIGLEAPEAAKEHFSHIR
                     RVAESIPHLRHDAILMLTEAYLLYKTGDIPKGARLLEAGLKKAKRHNTIHSTFWFPSV
                     LTDLCAYGLDGDIEVRYVQDIIRRTRLMPESSETVSENWPFAVKIHALGRFEIEKEGK
                     PLDFTGRVPQKPLALLKTIIAFGRLEVSMEQIADAIWPDAEGDAAHSAFTTTLSRLRK
                     LLGCEEAVRVRHGKVSLDPQLCWVDVWRFLHILDELDRVYRQCDCESRRQDRPMSDAL
                     LLDRLIHLTESGLALYKGPFLGDEPRHAHALVMRERLRTRHIRLILNSGKHLEKAGQW
                     RRAADLYLRGLETDALVEEFYAHLMICYHRMGLKADAAAVFKRCETTLMEAFDMEPSD
                     QTRRIYESLHHRS"
     gene            28029..28310
                     /locus_tag="PLM79_06370"
     CDS             28029..28310
                     /locus_tag="PLM79_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOV85969.1"
                     /translation="MFLYKGKPISDIRTGLTDACEAAGIPYGRFTKDGFIFHERHTFD
                     TSMRRAGVPESVIIEITGHSTREMFDRYNTVDHDDKRQAIDQMGVSAES"
     gene            complement(28688..29428)
                     /locus_tag="PLM79_06375"
     CDS             complement(28688..29428)
                     /locus_tag="PLM79_06375"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HOV85970.1"
                     /translation="MSMIAGGFQDWTKKPPVLREINEWHEQLDRLIEGMIQDYALLEA
                     ASPRVDFSQDEHSYHIRAELPGVAAKDIDVSVAGDSLVIRGERKSTDESRDECCHYRE
                     RVTGGFYREIPLPSDASPEDVRAELKDGVLTVTFKMSESRKPKIIKVKGEALPETHES
                     YRKRIESEIAEWTSRLAELRAKAGEKSQELRKKYREELDGLEDKLGELKYRLSNLLAA
                     SGEAWETMKDRLEEAMDDLKEKFRRIRR"
     gene            29936..30502
                     /locus_tag="PLM79_06380"
     CDS             29936..30502
                     /locus_tag="PLM79_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOV85971.1"
                     /translation="MVGYARMSPLEKDLQPQVNALKAAGVEERHLFTDETIEPRAEWS
                     GLHACLGELISGDTLIVWKIDRIGRSVPHLLELMENLHGRGVGFRSLCDLTIDTTGVF
                     GDVVTEVISCLARFSQRVLQERARAGVETGRARGRQGGRKPIASSNPRVRAAKAMSVN
                     GNIPVREICSTLRISRATYYRYLDLEEE"
     gene            31032..31214
                     /locus_tag="PLM79_06385"
     CDS             31032..31214
                     /locus_tag="PLM79_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85972.1"
                     /translation="MTLRRRGLLILFLVTACVGADQAAKEWARVRLSGSSALSLAGGV
                     LKLDYHENQGGVFSFE"
BASE COUNT         6620 a         8953 c         9036 g         6722 t
ORIGIN      
        1 ggggatatcg aggtccggct actactatga gccggcgatg gagagcccct ttaatctgat
       61 gctgatgcgc ctcatcgacg agcactacac tcgattccca ttctacggct caccgcgcat
      121 gacggcctgg ctgcgcagtg aagggtatgg ggtcaatcac aagcgcgtag agcgcctgat
      181 gagacagatg ggaatccagg gggtgtgtcc ccggcgaaac ttgagcaaag gatgtgatgg
      241 gcacaggaag tatccgtatc tgcttgccgg tctcaccgtg gaccatccca accaggtctg
      301 gtgctccgat atcacctaca tccgtctggt gaaaggattc gtttacctgg tggccatcat
      361 ggactggtac tcccgattcg tgctctcctg ggagatctcc aacaccctgg atacccattt
      421 ctgtatggaa tccctggaga gggccctgtt gaggggaacc cccgagatct tcaacaccga
      481 ccagggggtt cagttcacca gtgccgcctt cacctcccgg ctccttgacg aacagatccg
      541 catcagcatg gatggccggg gcagagcctt tgacaacatc ttcatcgagc ggctgtggcg
      601 atcggtcaaa tacgaggagg tctacctcaa agactatgag acggttatgg acgccgtcac
      661 gggaatcggc agttacctgg atctttacaa ccatgagagg ctgcacccgt ctcttggcta
      721 ccgcacaccg gcagaggtct atgggagctg attcattggt gggctccgcc ccccaagccc
      781 cccggagttt atcgctttag gggaatcaaa aggggcaggg gaaaagatgg gcggagtccc
      841 tgtcgggaag ccccgcccct ccgtcatccc cccacctcgg cgctcaggtt gctctccagc
      901 agtggcctta ccctccgggt gggcagaaga agcataacac agcccgttca gaaaggctgg
      961 tcggaggagg tgaaaaaaag cttaggttga cccgtttttt gtctagactt tggggggaac
     1021 cttactcaag tcagctcagg tgatgatcta tggaggacca tcaggcggaa ttcagaggca
     1081 ttttgcaaag aggctcagaa tcggaaaaaa ttccctcgtg tgaccttcct gtgacttttc
     1141 ctgtgatacc cagtttcaga aagctctacg atccgaccta gcttgatcgt gcagggatct
     1201 gcatttctgc cgcagacccg tgcggatccc ccaaagtcct ttgggaatgg agccgcgtcc
     1261 gtgaaggtca gtgggagtct gtggctgaag acgggctttt tgagcgtcca ccttgcgcgg
     1321 caaggcccgg ccggggggac ttcctccccc agaaggagcc agaaatggtc agttacatcg
     1381 tccgcatcta tcgcagagcc gatgccttgg agggccgggc cgcgggcatc gcggtcgatc
     1441 cggaaacggg agaggaagtc ggcttttcaa acgcccagga actctggtat atcgtcaatc
     1501 gggaccgctc cgcagcccct gtgaccgccg cggaagaaaa ggaggaggaa aagagatgaa
     1561 agccatccgc cgatcgatga atcccttcat cctgaccggc catgaatcct tcggcctctg
     1621 agagaactgg ggggaccaaa ttggaacgag atgcagggcg ctctctgttg ctgcaggcat
     1681 atgccgggaa ggctttccta ccggtggcat gctcgttcgt ggaacagtcg gcgatagcgt
     1741 tgggaatggg gaaacgtgaa gcgctcggcc tgacgcttgc caccgaggaa gtcttcgccc
     1801 acctctgcgc agtggtcttg aaagatgggg aaaaagtcga gatccactgc acaaacggcg
     1861 gatactacgt tcaggtcgac ttcacatttc ccgcttcgga catggatctg cgcgctttca
     1921 acctcaacgc gacggtgtcc tttgcagacg atgcggatct ggaagagatg gggctcgtgc
     1981 tggcctccag atcggtggac cgctttgccg taacccggct ggaaggcaag gggctgaagc
     2041 tgaccctgct caaagaaaaa gcgtatccgc ctttcgacga atctcccgtg gcgatcccca
     2101 ggcccatgga caggttttcg gtccggccgc cgaaccctga agaactgaag cttttcgcgc
     2161 atcttgtgaa gacatgctac ggaaaccagg tgcttcccaa ggttttcgac catcccggaa
     2221 agctggtgga catgctggcc ggggccaatt acggcgcggc cgtggccgtg ggtccggcgg
     2281 ggggaacagg cggcggcact ttctggcatc gagcgggaga gaaggcggtc gaatgcttcg
     2341 gcccatattt gttcaaccag aaagccggct cgcccatccc cggcgctctc cttgaaagct
     2401 gtgtcggagc gattgccagg acttccgcgc tggtcctcat caatcgattt gccacacctg
     2461 aatttgcgcg ggagcatttc gagcccctgg gcagtgtccg gatgcacacc agcgagggcg
     2521 agctctcgcg cagggaagca tggtttcggc taatgcatga ggatccggga agtatcgcct
     2581 gggtacatcc ggagctcgaa acctttctcc agcaggaata ccggaggctg gtgctgcctc
     2641 gcgaattgag gcaggtcaca aacgcgggag aacagcaccc ccggcactcc gtaatctcca
     2701 ccaccttcga tcgaccgcag gcaagcgcaa cactgcgccc gatctggttc ggaacggact
     2761 ttgccagtaa ccttgtccag catctgaagc tcctccgcca ggaggggatt ctgaacatat
     2821 tcttctcctt ggacgtcgga aaatcctggc aggctgcctt cacccccggc cttctccaaa
     2881 acggtttcac gccgtgcctc atctttccct gctccggcga gggagacatg gtgcttttcc
     2941 agtttcaagg agcgacccca tgaaaaactt gcctcctctg gaccgtctgg tgccgcctca
     3001 tatcaggcag ttccaggctt acattcccag caagcccgat ggtgagctga agaagatgta
     3061 cagatgcccg cggctctacc ggctcaacaa caacgagaac ttcctcggtc ctccccccgc
     3121 cgcccttgag gccattcgcg catttccgtc gccacaggcc gccgtctatc ccagcggcga
     3181 cgcctactac ctgaggtgca ggctggccga ccgattcggc atgcatccgg accagttcct
     3241 ggtggggaac ggcgcaaacg aggtgatcag cttcgtcatc aaggcattct gccgtgaggg
     3301 ggacaatatc atcaccgcgg acaagacctt cgcagtctac gagtgggtgg cggagttttc
     3361 gggtttcgaa gcccggctgg tgcccctcgt gaatttcggg ttcgacgacg agggaatgct
     3421 caaaagaata gatgagcgca ccaagatgct tttcgtatgc aaccccaaca atcccacggg
     3481 aagcatttgg accacggagc gcttgtgccg gtttctagat cgcatcggcg gacggcagat
     3541 cgtggtggtg gacgaggcct atgccgagtt cgtggaagac ccggcctacc cggatgggat
     3601 ggcgctcatt cgtgaatacc ccaacctggt cgtcttcagg actttttcca agatgtacgc
     3661 cctggccggc ctgcgaatcg ggtatctggc gggaagcaag gaggtggtgg atgtgatgag
     3721 gcgcacctgc atcgtctatt ccgtcaactc cctggcccag gcggcggcag tggccgccct
     3781 cgaggacagc ggcgatcact cggccaaaac ccgtgagctc atcaggcagg gaaaggcttt
     3841 cttgaaaaag gagatagaag ccaccgggct tccatatgtt ctgggcgaag gcaacttcat
     3901 gatgatcaag ctgccgatga acgacacact ggcctaccgg aagctcatga cccatggagt
     3961 catggtgcgc agcatgacct gctttcgctt ccccaattgg atacgggtca ccatatcgca
     4021 ccaggaagcc atggaggcat ttgtcgaggc cctgggaaag gtgcttgccg ggggagcagt
     4081 ccggaaatga gatggaacca ataggttttg agttttaagt tttaagtatt atgcagagag
     4141 gcgcggccca aggcacgtca tgagtgaaat cctactgaat gccatactgc atcttttcgc
     4201 catacaggca tccctgttgg accatctatc cagggccagg gcacgtgccg tggtcgagcg
     4261 gtatcttgag acaaatctgc ggattgccga tcctggcccc tacctggatt tatatgatat
     4321 gctccttgaa ttccaagagg atagagacgg ccgggcgcgc ctggagcagg caagggaaat
     4381 cgctcggaag ttgaagcccg tgctgtcacg cttcgatcag tatgcctttt tgctgcacta
     4441 tctcacgctt gccgccaagg tcggcgcggc tggagccgcc atgggggcag ccgaggccgt
     4501 ggccctggag ctggcaatcc cctgggagcg gctggatgat ttgcgcatct tttccgaatc
     4561 tcccttcgat cccgcacgtc tgacggagag ctttcttgtc cacgatccgg aggattgcct
     4621 cgggcagatg caatgccgga ggattgtgcg gccggatttc aagggcggct tcacggtctt
     4681 acgcctcagc gatgtggaga cctgcttcat cctcccggcc gaagacagcc ggattatcct
     4741 cgactcttcg cccatggaga gcggcacgct ccatatcgtg cggcccggag ccattttgcg
     4801 ggacgctttc ggcactcaga tctaccatgc cgaaatcgag gccgcattca atcgatccgc
     4861 aacaggcgtg ggagtgctct tttgcggaga ggacctcaat tttcgctatc ccggcagcga
     4921 caacggtctg cataatttca gcttcagcgt caaaggcggc ggaatgatcg gcatcatggg
     4981 cgtgagcggc tcggggaaga ccactctgct caatatcctc accgggcgct cccggccgca
     5041 atccggccgt gtcctgatcg acggcatcga tctgcatatc gagcccgaac ggctcgaagg
     5101 cgtaatcggc tatgtgcccc aggacgatct tctcttcgag gatctgagcg tctttgaaaa
     5161 cctctattac aacgcctccc tctgcctcgc aaacctcgac ccggccgaac gggcggaccg
     5221 ggtggatgcg cttctggacg agctgcatca attgtccatt cgcaatctca aggtgggctc
     5281 ccccactgaa aagagcatca gcggaggaca acgcaagcgg ctgaacatcg ccctcgagct
     5341 cattcgcgag ccgtccatcc ttttcgtgga cgagcccacc tccgggctct cttcgtcgga
     5401 ctcggaaaat gtaatggccc tgctcaaggc gcaggccgcc aagggcaagc tggtcattgt
     5461 ggtcattcac cagccgtcat cccgcatttt caagctgttc gacaccctgt ggatcctgga
     5521 ccagggaggc agacccatct ttgacggcaa tcctctcgat tccatcgtct atttccggac
     5581 ctccgtgcac caggccggca tgggggagta cgcgtgcccc cgttgcggca atgtcaatcc
     5641 cgagcagctc ttcgagatca tcgaagccaa agccgtggat gaaaatggat attatacacg
     5701 gcagcgcctc atatcgcctg gggaatggca tcaacgttac ctggatcaca ggcgcggaca
     5761 tgccggaagc aatggaggtg aagccgaaag ctcctccaac acggagaaaa ggctctggcg
     5821 gcccggccgc ttgggacagg tgtgcgtgtt cttcatgcgc acgcttaaaa gccgcttgtc
     5881 caatcatcaa tatgttttca tcaacctcgt cgagcccgtt gttctggctt tgcttgccgc
     5941 attcatcacg cacggcgccc gcggcgggga gtatgttttc atggaaaacg gaagcctcgc
     6001 cacctatttc ttcatcagtg tcgtcgtggc tcttttcctc gggttgagcg tcagtgccga
     6061 ggaaatcaac cgggaccgca gcattttgca gcgcgagagc ttcctgcacc tgagttggcc
     6121 ttgttatatc atggccaaat cgctgtacct gatgcttgtc accgccatac agatggcgct
     6181 ctatgtctcg ttcggaaatg gtgtcctgga aattccggac atgttcccga ccatgttcct
     6241 ggtgcttttt tcgtgcggcg ccgtctcctg tgtgctgggg ctcaacatct ccgccgcgct
     6301 caagtccgcc gtggccattt atattctcat accggtgctt ttggtgcctc agatcatgct
     6361 gggcggcgcc gtggtccccc ttgaagatct catccaccgg caggccggca accggtatac
     6421 gcctctcgcc gcggactgca tgccttcccg ctgggggttg gaagccctta tcgtgaaaca
     6481 gtacgtttca aaccgctaca tgaggaactt cttcgaggat gattgctcga tcaagcaatg
     6541 cgattacatg ctctcctttc atttgccgga gatgagcgca ctggcggcct atccctttct
     6601 ggaaaccgac gttccggaca agccgtctca aattgcacgc aaactgacca ccctgggaaa
     6661 cgagatacgt ttgctcgaac aaagcacggg attgtcttcc ggcatacagg cggatgtctt
     6721 caccccttcc ggctttactc cggaggtgga gcgcctggca aggggatacc tggaaactgt
     6781 cgccgagaaa taccgcgccg cgcgaaaggc atctttgaag cagaagcagg aaacggaaga
     6841 cgccttgcgc agccggttgg gcaggaaggg attgagcgcg cttaaggagg cccactacaa
     6901 cgagagcatc gccgatatcg cgctccgcac aaaagtcctg gatgcgattc gtctttccgg
     6961 cggcaggttg gtgcagactg ccgcgcccat ctgccaaaca ccggaatcaa attggggaaa
     7021 ggcacatttt ccggctgcgc ggaagcggat cggctcctgg ttgattcccg cccacgtgtt
     7081 caacgtcgtc gtgctttggg cgatgacggc gctgctttac ctggcccttt atttttccgt
     7141 tttgcccgca atactgagct cagcggaatc cgccgcaaac cgtttgcggc gtcgcaagag
     7201 gccctagcac tccgtcaatt tagttttgcg gggttaccga cagactttgt accgtcattc
     7261 cggctaaaag actgccggaa tgacgaatag gcgctcggca atcagtacgc gcaaaacaat
     7321 gttgacagag cactagtacc ataatcaatc attatcgccg gacgtcaaat ggagacagag
     7381 ccggcagaaa agaaggggaa gtaatgatca tgaacggagc tattgcaggg aatgtcgtga
     7441 agattgttgt ggttgtcctt ttgacgtcgg cattttggtc attcagccac gccggggagg
     7501 gagatctgaa agcggctgcc aaagtgctgg ccgatggcgt ccagccgaca aaagagattt
     7561 cggatcatct tgaactgtac aagaaggcag gaattaccta tcaaccggat ctcccggtcc
     7621 ccctgaagga tatcgccgac tgcaagagcc aagagcagtt gcgcgttctc atgggaatgt
     7681 atgcttttga gctcggatac gcgatggctt tcgagaaaag aaaggagctt ctggaaacga
     7741 gcagggcaat acaaagcgaa atcgtggaca ggcttgaaat acgcgataag cttgacgtct
     7801 cgcttgcaaa ggcggaaccg ctcgggtccg acaaggaaga agccgacgga agggggagag
     7861 acgaactgct cgagaaccag aagaactgga tcatgaatat gatcgaaaaa gcggaaaacg
     7921 atccgcaggt aatggaactg ctgatggata taatgtacgg gggctcgatc gagggcctgc
     7981 acgtgatctg caatatagcg ctcaacgcgg aaaaaggtga tgctctgatc gcgctcctca
     8041 accagcgtgc ttccagactg gaaattatgg atgccgccct gaaatgtttc aacgacaagg
     8101 atctggcctc aaagatgaaa aaggacagcc gcgtaaaatt cagccgcgcc gttatcgatc
     8161 tggtgaaagc aaaaaagggc gcgctcacac gggaagacat gcagcaaatc ctggcgctgg
     8221 tggagcccga acggaatatc tacgtaaaga aatgcgaata gatatcgagt gatcgcctcg
     8281 ttggggagtc ggcatccatg tctgatttcc agcggaggaa ccgcaacgat tcggcctccg
     8341 tctcgtgccc atgtgtataa actgcctccc gagctgcttg cggatatcgc ttgttcttac
     8401 caagacgaga acggctcgca gcgtcatctc ggcgagggtc ccggtttcaa aaccatggaa
     8461 gaaaaatggc ggcagtagta cgatgtcttt cggactcaag attccgggag cgagccggtt
     8521 tttctgcatg tgctttccag cccgggagag ccaaggaaaa tactttatga cacacgggcc
     8581 tgcgccaggg aaaccaaggt catccttacc gatctggagg ctgacatgct ggaatgctgc
     8641 gaccgggttt gcacaccgcg gcagatcgag gagtattgcg cccgatgcgg ccaccatcat
     8701 ttcggggccg tctcgaaagc tattgagaat ctgctgaagc gcaaagtgct tctccaccag
     8761 gatgggagct atctctccct ggcggcccac ccgccccggc gggcctacgt gccgctcggt
     8821 gatttccccg gagggtatta ctacccggaa tcaggtcccc ccgtttgcga ttgaaaaacc
     8881 ggctcaccga gtttctggca cgcttcctgc tcatcgtcgt tacgagctca accgacatgc
     8941 tgcgcagcat gacctgcttt cgcttcccgg actggatacg ggtcaccatc tcccgccagg
     9001 aagccatgga agcttttgtg gaggcgctgg cggggatcat acgtgagtga gggcaggcaa
     9061 ctgcgtgctg gaacagcgct gcgtaagcca tgccagtgag gcagagcctg ggagcgatcg
     9121 gaaaggaatc tgagctcgaa accctggaaa tcttctaacc ggaggtactg cagtgagtgt
     9181 gtggacagga cttttcatcc cggccgcttt ggcatcgatg gagcttgcgg cgagagctta
     9241 ccatcatcgc cggtatggcc ttcctttcaa aagccgggtc tttgccgaat acccctacca
     9301 tgaattcgtt gaaaaagcag acccgccctt tcatttcaga ctcaagaagg gataccgatc
     9361 cccttcggtc aatatcaacc agcacgggat gagaggcccc gagctcaact cacgccccgg
     9421 agtcaagagg gcattgttca tgggcgagtc gcacctcttt ggcgtcaagc ttttccacga
     9481 gcgcgatctc tggtccttcc ggctgaaagg cctcctcgac gaaagggcag attccgactg
     9541 ggagatcttg aatttcagct ttcccggcta caacacccac cagtacaagg ctttttttga
     9601 gcacgaactg ctcggcctca aacccgatct ccttgttctc agcatcggtg cgaacgacat
     9661 cactcaagcc cggatattcg gggaaaaatg gcaccccggc atggtctacc cgctcgattt
     9721 catctgtgcg ctggccagga agagtccctg gtgggaaaag ctcctgaacc gaagctgcct
     9781 ctgttcttcg tggctccgga aagctaggga ggcacaacgg agtgcctttt tcaaaaccgg
     9841 tgcttctttt ggcaaggaag aatgcttcca ggccgctttc acaaatatcg agctcattgc
     9901 caggagcgcc caggcttgtg gcgtcagggt gatcttcatg ctcctttgcc ctgtgtacga
     9961 gcttctaatg tctgctgaag accagcgaaa gatcacatct cttctgtcgg actggcgtcg
    10021 gtacgtcgat ggcgacggtc cagccatgtt tgagttcatg gaacggatga actccgaggt
    10081 tgccccacgg cttgacatgc ctgtaatcga ccttcgcccc agattctggc gtcatccgaa
    10141 caggttcaag ctctattttg acctgtatca cctcaatccg aatgggatgc ggctgttagc
    10201 cgagtctctg tacgaggaaa tccataagct cggctgctgg tgagagtgct gaacatgttc
    10261 cattcaaggc acaaggacct ggacccaaca cagcccacga acgtaaccat cgcttgacga
    10321 attggcggag cagagagtgc ccccatggaa gagcccaaaa gactgtacac cttcgagttt
    10381 atctccctgt gcttcgtgtc ttttttcggc ttctgcaaca tgtccgtgtt ttacagcttt
    10441 ttcagctacc tcggccgaat cggcattccg gaggaatgga gaggctttct ggtggggctc
    10501 gagcccatga gcgcctttgc cctcaagctc gccataatcc ccctgttgca catgggcaat
    10561 gccgcgagag ccatgcttat cgcactcctc atgaccatcg tggcgctctg ctcctatacg
    10621 ttggccgtga ccgtccccgc cctcatcatc cttcgcatct ttcacggggc tgcgttcgtc
    10681 ctgctcgtgt cagcggccat ggccttcgcc gctcatctca ttcccaggga gcgaagcgcc
    10741 caggggtttg gaatcttgag cataacggcc acgctcccct atgcggtgat gccccttgtt
    10801 accgaagccc tgctgccgta cgtgaagaac gagagcacta tctacgccgg tgtttccgtt
    10861 cttgcggtgc ccggcatcat cgtgctttgc gtactgcgaa agcgcatggg cgcattgttt
    10921 gccggtgtcc acgcttccct cgcgcgacgc ccgagcctgg acgaactccg ccggaacctg
    10981 aagctcccgg ccgtggtcct gatcctggcc gtcagcctga ttctctactt cggcaatgcc
    11041 acgctcttct tctttgtcaa gggctacttc aaggagatcg gaacagccaa cgccggccac
    11101 tttttcaccg cctcgacact cgttatgatc gcggtccgcg ccctgggggg acggcttatg
    11161 gacaaggccg acaaggtcaa gaccatggcc ttcttcacgt ttctcctcgt tccatggttc
    11221 atggccttcg gctatctccg ctcgccgatg ccgttcattc tgctcgccgc atgcttcggc
    11281 ctgtgcgcag gcatagtctt tcccatgctg agctccgcca tgctccaggc ttcccccgcg
    11341 cagcttcgcg ggctgaacac gaaccttgcg ctcttcatga tggatacgag cttctttctc
    11401 agcccctatg ccgccgggat gctcttcgcg gggggcggct cgttcgaaga tctttttcat
    11461 atttgctcgg ggcttctcgc tttggccgtc tgtttgctcg tggccctcgg aaggcttgat
    11521 tggcaatcga agtactggca gtgcacaccc tgaaccatga aaggaataca aaagcataga
    11581 aaatgaacaa gcaggaagcc atccgaaaac tcagggaaga agcttatcaa gtaaagcccg
    11641 accaggaatt cgaagtccgc aggatggagc ccgaagacgc ctggggcgtg gttcgctgca
    11701 actttgcggt gtacggcgag cactatccca tcgataccaa ttacatcccg gaaaggctga
    11761 tcgaggaaaa ccgcctcagg aacctttata ccgtcgtggc gcggaccggg aacggagaca
    11821 tcataggctg cggtgctctt tacaggagct cggcctattc ccccagggtc tatgagtacg
    11881 gccaggccat agtccacccg gagtatcgcc atacctttgc ggttttatgc attcaggact
    11941 acatattgaa tgttcttgta tcccaggaag acatcgacga agtgttcgga gaagccgtgt
    12001 gcaaccatct cgtcacccaa aagttggcgg cattggcgga cttcagggaa acgggcctgg
    12061 aggtgggggt gatgtccgcc gaggcctatc cgagcgagga ttttcccggt gagcgtgttt
    12121 ccacggtcct tcacttcaga agcgtgcggg acaggatgca ggaggtatac gtgccccggc
    12181 agtacgagca ggtgctggaa tacatcttct ccggactgga gatatcgaga agcgttttgc
    12241 cgtccgaggc aagagcgccg tccggctccg ccacggggat aaagacccag atattcgatt
    12301 tcgcccaggt ggctcgattc aatgtctaca gcctgggaga ggatttcccc gccgtgctcg
    12361 cggccgagga ggaaaaggct cgaagccgcg gcacagaagt gatgcaaatg ttcatcaacc
    12421 tgggagaggc ctgcgcgggc cgggccgtgg actacttgag ggacaagggc tacttcttcg
    12481 gcggctttct gccccgttgg ttcgatacgg acggcttgtt catgcaaaag ctggtcaagc
    12541 tgccaaactt cggctccatc aagctggact ccgagcgggc acacaagatc ctcggcctca
    12601 tccgccggga catcgagggg aacccggcct gccgcgcgct gctgagccgt tcatgctgga
    12661 ggtgatggat gccttccggt tctcatgtca tgcttgatct gtacgggttt atgctcacgg
    12721 ctcgaatcct cgacgagttc gaatcgaggc ttactcggag cggcgaggtc tcttttcaca
    12781 gctccggcgc cgggcatgaa tccacggctg cgctggcttt ccatctcggc ccttcggatt
    12841 ggctgcactg ccattaccgg gacacggccc tcatgatcgc aaggggcgta tccccggagg
    12901 ccttcttctc gagcctcctc tgcaaagaca cctctcattc acgtggtcgg caattgaatg
    12961 tgatgatgag ctgccccgag ctgaacctcc tgagcatcgt gactccggtc ggaaacggcg
    13021 cgctgcaatc ggtgggggtt gcatcggcaa tacgtgacga caaggaaaat cccattgtcc
    13081 tctgcggcct tggtgatggg atgacccagc agggcgaggt gtgcgaggcg atcggagagg
    13141 cagccaggga ctcgtgtccc gtgctttttc ttatcgagga caatcagtat gctctctcaa
    13201 cgagaaccag ggggcgcacg ttctatgaca tggggggtga gcgcgcagcc tctttctatg
    13261 gtgtaccgat cttgtattgc agcgggtcgg aagccgtcga gtgctatggg atcttcggaa
    13321 atatcgtgag tgagatccgc cggacgagaa agcctcgtat tctggttttg catgtccctc
    13381 gcctgtccga ccactccaac gcggaccgcc aggctgccta tcgctcccct gatgaactcc
    13441 tcgaagccca ggcgctcgat cccatcccga gactcgagaa gcaccttttg cgctcgggca
    13501 tctccgaggc cgagctcaga ctcattcgtg tggaagccga agagagaata cagaaagccg
    13561 ccgatgcggc ccgaaatgct cctgatccgc agccgtgctt cagcgcaaag gcgccgctgc
    13621 ccgattgtct cagccgccag agctttcccg agccccttta tggctcccgg gacgggttga
    13681 cgatggtcga ggcgatccgc gaggtcttgc ggagccatct cgagaaaaac gacaaagtga
    13741 ccctgtttgg agaggacatc gaagacccga agggggatgt cttcggggtt accaaggggc
    13801 tttcgaccca gtttccagga agggtaagga actcgcccct gagtgaatcc accatcgtcg
    13861 gcaaagccat cgggcaggca cttgctggag gccgccccgt ggcctttctc caattcgcgg
    13921 atttcatccc cctggctttc aaccagatca tgaacgaact aggaagcatg tactggcgaa
    13981 cggatggcgg gtggcaggcg ccagtcattg tgatgatcgc ctgtggaggc taccgcccag
    14041 ggctcgggcc ttttcacgct caaaccctcg aatccattgc cgctcatgtt cctggaatcg
    14101 acgtgatgat gccgtccaat gccgcggatg cagcggggct ccttaatgcc gcctttaagt
    14161 caaatcgccc gaccctcttt ttctatccaa aatccctgct taatgacagg cagtcaactc
    14221 tcgatgcgga tgccggtgaa gtcatcgtgc ccatcggccg ggcgaggatc acgagagaag
    14281 gcaaagacct gacttttgtc ggatggggga acaccgttcg gctgtgcgag caggtggcgg
    14341 aaaccctgtc ggagcatggc ttttcagcgg agatcgtgga cctgcgcact ctctcgccgc
    14401 tggacgagga aaccattcaa cggtcagcgg aaaagacggg gcgaatcatt gccgttcatg
    14461 aggacaacag gacgtgcggt cttgcggccg aagtcctggc atgcgtttca gagcgtttga
    14521 agacacatgt tcgcatgacc cgcgttacgc gcccggatac ctatgtgccc tgcaattatg
    14581 caaatcagct cagcatcctg ccctcctgcg aatccattct tgaagccgcg gcggagctcc
    14641 ttgacttctc cgtcgagtgg cggacagagg aagaagaaga tgcttctctt gtcttcgtca
    14701 aggccctggc tctcagcccc gccgacgagt tctgtatcgt tgtcgagcta tacgtaaagg
    14761 ccggagacaa ggtgaaggaa ggagatgtca tcgccgcggt cgagacgaac aaggcagtgg
    14821 cggagttagc ctcaccggct tcaggcgtca tcgaggatgt ttttgtcgaa gaaggccaga
    14881 ctgtcgctgt cgatgaaagg attttgagca tccgggtgaa gccggatgac ggcgccatca
    14941 ggcgaaagga gcctgcaaca aggaggagcg ccgttctttc aaaaagaacc tctgagatga
    15001 gcgagaagac aacctctcaa atctccaccg cggtgccggc ccgtgcaaca ccggtataca
    15061 tctccggtgt ggcttcttct ctgggatcga ggacgatcca taacgagacg cttttgaaga
    15121 gcttccctga ttggtcaacc caggacgtca tcaaacgcat cggcatcgaa tcccgaaaat
    15181 ggattggaga aggagaatcc gcattgactc ttgctttggc ggcgtgcaca tcgctcttcg
    15241 agaagctgcc tgtcgggttc gaggacatca gtgcaatcgt ctgcagcaca ggtacaccga
    15301 tctcggccag tccctccatg gcctgcgaga ttctcacggc cctcgcaggc aataggagtg
    15361 ataagctcgt gcaggccttt gacatcaatg cggcctgctc cgggtatctc tatggactgc
    15421 aggtggcgca cgacatgatc caaagcagtc tccaatccca cgttcttttg gtcaccaccg
    15481 aggtgctctc gcccctgctg aacctccagg atcaatcgac tgtcttcatc ttcgccgatg
    15541 cggcaacagc cacgctcatt tcctcgtctc ctcttgcggc gaaatcggca agggtgaaac
    15601 gtcccgtgct ttccgcaaaa ggcgatgtgg cgcgcacctt gagtattccc tttattggcg
    15661 gcgcggcggc cttcatcaca atggaaggtg tagaggtggc aatagaagcc ggcaaaggga
    15721 tgtcgttcat ccttaccgag gcctgttctc gagtcgggct cacccccaac gatctggatc
    15781 ttgtgatccc tcaccaggcg aacaaaagaa ttctcgcggc ggtgcaacgg cgactgaacc
    15841 tgcctgaagg caaggtctac accaacttga aagacatagg aaacacgtca tccagcacaa
    15901 tccccatttg cctggatcat gtactgccgc ggatggaacc cgaaagcagg atcggcctcg
    15961 tggctttcgg agctggattc acctttggag ctgcgattct tgagactgtt tgacacgtat
    16021 atcacggggt ttcgactgtg tggccggctt ccggggagaa agttatgagt gaggacgaga
    16081 tagtattgaa gttttccgag atagtcttga atgtgcttgg acagcctttt gagctcctgg
    16141 ggctgaatga aaacctgcgc gagagccgtg gggcagattc catggacatg gtggatattc
    16201 tcgaggctac cgagcttgca ttcggcattg atgcgggaga cgggagctac gagaaaatgg
    16261 tgaatgtggg gcaggcggtc agcctgataa agaagcttat agagtcgagc cgtcaaagcg
    16321 gccgatgatg gaagccgagt tgatgcctcc aaatccaaag gaattgttca acaggcagcg
    16381 caccggatgc tccaccgatc cgttggcgca gacgtcgagg tcgatttccg gatcgagccg
    16441 gtcgatgttg atggagggat ggagacggcc ggcccgcatc tgcaggaccg cggccacggt
    16501 ctcgaccacg gccgcggact cgagagtgtg tcccagcatt gatttggggg cgttgatctt
    16561 gagcttcccg gcgtggtccc caaaggcgcg cttaatggca cggatttccg ttacgtctcc
    16621 caccacagtg gacgtggcgt gcgccgacac aaaatctatc tcctgccggt cgataccggc
    16681 tttccgcact acctgctcca tgaccagggc ttcgtgctct tcagaaggat aggggctccg
    16741 cgaagccgcc gacctgagct cggctccgag aatctcgccg tagatccgag ccccgcgggc
    16801 cagggcgtgg cccaattcct cgacaactag tgccccggag ccgtgggagg caacgaagcc
    16861 ctcccggccg acgtcgaaag ggcgactcgc cctggccggt tcatcgttga agctggtgca
    16921 cgaaatggcc cccaggcgag caaacgagtg cagagtgacc gggctgaact catagacggc
    16981 gccgaccacc agtgcgatat ccacctcgtg atggcggatt tcgtccatgg cgctgcgcag
    17041 agcgacattg cccgaggcgc aggctccccc gacggtatag atcgggccgt ggatgccgag
    17101 gatctccgcg acacagcctg catggtcggt gtctactcct ttgagagcga gcagggcatc
    17161 gctgtaatcc gggtactcga caaacctctc ccaattctga tgatagtact gctcattcaa
    17221 gttgtgaccg gcaattatgg ccgcgagcct ctccggattc aaaaccgggg tgaagagatc
    17281 cgcgtccaga aacgcggcca cggccaccag cacggaaagc tgcagcgacc agggagagtt
    17341 tcgaatcagc ctgggcaggc gggaggccgt ttcatccggg atctgttccc gtagcgatga
    17401 gatcttgccc gaaacatcgt atgcatacag ctcgccgccg atcttgctgc tgatccgtga
    17461 tgaatccagg agcttccagt gcgtaatcgc ggaccgacca tcgaggaggt tttgcagaaa
    17521 ttcgtccagg gtgtcgccaa gcggcgtatt gatcgacata cccgtgatga cgatacgtct
    17581 tttctcagcc aaagtacccc tccatgacgg ctgcggcgca atgagtctcg ccccgcgata
    17641 cctgtccggc aaaaacgacg gtgagcccgt tgctctcgag ggccagggca cgcaacgtca
    17701 cctcgtcacc cggcataacc ggagcgaaaa agacggatgc atggatgcgg aacaggcgca
    17761 catccggggg agcggggtca gtgaagtctt catctttgtc tttgcggctc tgtaatccga
    17821 tcatgcaaac gccgagctgc cccatcatct cgatcagaag cacccccgga tagatcggtt
    17881 tacccggaaa atgaccggcg agcaccggat cctccggatc gatccggcgt attccctcga
    17941 tggcttcctg ctcgtagtca atcgcggtaa tggcgtccag cagcaggaat ggatcacgat
    18001 gggggagcag ccgttcaatc tccgcccgcc ccaggctcac caggcgcgtg gctcggcctc
    18061 gacgccagag ggcctggcgt gaagcttgac gcaggaactg atccttgtct tcgtaaagcg
    18121 gcattccgat actcctgaaa aaactgcctg accggatcga tggcagcgga tcgggacaag
    18181 gaacagcacc tcgatccgct gctccgttcg actgatgcgg caacgtgccg ggaatatcgg
    18241 cctatgcctg taattcgctc ttcactttga acaggtaatc gaccaggccg tttatcgtac
    18301 tgatcttggc aaactcggcg atagggatct tcatctgcat atcccggatg atcccggtca
    18361 taatctccac cagatccagg ctcttgacgc ccaggtcccg ataggacttg ttcagatcgg
    18421 tctccgttcc ctgaaacgtc ggcactacct ctttcacgtt cctgattacc atggccaaaa
    18481 catcatcacg tgtcatctac ccattccttt cttttgcggt aaatcccaga ggccgaaaac
    18541 atggatccgg ttcttgccgg tgtgacgatt ggcaaaccgc cccctctttt gacttcgtcg
    18601 cccctaccgg gcacgaagcg tgccaatgat agcgtcatcc gttccgagca caaggcaatg
    18661 ctttcaggtt gttgacgagg gtgagagttc tgccggcggc cggcaggaaa aaaacgacta
    18721 agcccggcaa gccgccgccc tcgtacgcag gacggtttgc cccattagtg acgttcgggc
    18781 cgatgccgag taccgtcgag ctctttccgg caaccttttg ctgcggctgg ttgctcggcc
    18841 ggtatctttt gccaggcgcg gatcaaaata gtacaccacc ggtggaccga tttcgatctt
    18901 cccaaaatcg ggatgagcgg gattgagcag atagttgaac tcttcgggaa ccacgatgct
    18961 gggaaccttc aagacagccg aacgttgatc cttaacccat tggtcgccca gcgcccttgt
    19021 ggcgggtgaa acagggcggc tttgccaatc tcccggcagg ctggagatcg ggatgctttc
    19081 aacgagatcc ggtgtgaaac acaccggaat gcggacaaat gctcccaaaa gcttgggtga
    19141 gggcaggtta acaatgattt ccagggaggc cagcgccagg ctgtcggccg tgtatacgac
    19201 cggcacaccc ttgctgttcc agcgcccgcc gtaaaggctg gctccctcgc ctgaaaaagc
    19261 tgcgtcttga tacttggctt cgacaaggcg ccaggccgtg aggatcacgt aaatatcccg
    19321 tgctcgatgc ggcccaacag atcttccacc tctttggccc ccaattcggt ggcggcaaat
    19381 tccaggggcg gaacgtcccc caaggcccag gcaggctcct tgagccattt gcgcccgagt
    19441 tccggctgtt tgaacacctc cacagccctg tcataaagac gggcaatgcg aaaaacccgc
    19501 tcagactctt caaatgagag ctgcccgctt ttctttcgtc tcgccagagt gctcagggga
    19561 attcctaaga tacgacagag ttccttttcc ggtatcgtca gattccggca taactgctcg
    19621 aacagctcgg tcggcagccc tttcttgatc ctggcgatca attgggtcaa gttctcgggt
    19681 tctaaaccga tggatgtcgc acgcataata acctcgcctc ctgtaattgt cattttgaaa
    19741 tatggtaatt tccatatggg aatttgtaaa gcggaaagca aggacggaat tctgttcctg
    19801 ctgcgcgaac gttctgcagc aggttgccta cggccaggac ttttcctcac tcgttccggt
    19861 cgtttcgtcc gattcgtctt gccctcgccg cagggacccg attattgtgc gaccatctgc
    19921 cggaaaaagc cgggaagagt gccgcccggc gccatgcgaa gagagccggg atcgggcaat
    19981 ctcttccagg tcattacacg acggctgacc atgcaaggct ttatcgtcat ggaccacatg
    20041 gacaaacgag cccggttcct tgccgatatg ggcaactgga ttcggcaggg caggatcaaa
    20101 tggaatgaga tgatcgttgg tgggatcgag aacgctccaa aggcattcat aggcctgttc
    20161 aaaggggagg acctgagaaa gatgctcgta aagatcgggc ctgatccggc aacctgattc
    20221 gaggagactc atcatgtcag ccgaacctga aaatccttgg aaagagtggc ttcttgaagt
    20281 cgtttggcag cttcagccgc ttgccgagcc cgtgcgtgag ccctgggtcc cccggccttc
    20341 cttgacggaa atccacgatt tactcctccc ggaagcggcc atcctgatgg cccaaccggg
    20401 catcgcggcc tatgagcaaa cactggcaaa gctcgagaaa gccggtatcg cttatgtgct
    20461 gaaggccttg gagggtgccg gatggaagtt caattccggg aaacgcttct ctacaattga
    20521 aatggcattg caggtgggag tggtcgatcc ttatcatcga ctgctcgagc gtttactgga
    20581 aatgttgtcg gaagaaggca tcctccaacg tttggatggc gcctgggaag tggtttcgga
    20641 acccgagccg ggagacccgg aagcatatat gctcgggctc tcctgtccgg gcgccgaggc
    20701 ggagatagcc ttgctcgagc gttgtggtcc ggaattggct cgagttcttc agggcaggtc
    20761 ctacccgttg gaattgcttt ttcctgaagg tgatacggct ctgctcgccg ggttctatga
    20821 acactcaccc gtgcctgaaa tgatgaacgg actggcccaa agggcggttc tttcaatatt
    20881 ggatcgcctg cctcgggacc attgccgccg gatactggaa atcggggcgg gaaccgggag
    20941 tacgaccgca cgccttctgc cgcacctgcc ggatgaccgg actgaatatg tgttcaccga
    21001 tgtctcccac ctgtttttga atggagcacg gaagcggttt caagaacatc ccttcgtcag
    21061 ctatcggctc ctggacattg aacgagatcc ccaaagccag ggcttcgagc ccggccggtt
    21121 cgacctggtt ctggcttcca atgtgctgca cgccacgcgg gatttgcgcg agagtttgca
    21181 gcacgttcgg cagttactgg cctcgggggg cgtgctgatg ttgctggaga caacgggggc
    21241 cgtgcgatgg cttgatctgg tatttggttt gacggagggg tggtggcgat tttccgatca
    21301 cgaccttcgc ccctcctctc ctcatctgtc ggcagggcag tggaaaacgc ttctcgatag
    21361 tcatggcttt gaagatactg tgtccatctc gcccgatgat cccccgaaat acggcttgat
    21421 tcccgccgga cgaaatctgc ttcccgaatc cttgatcctg gctcggaaga cggccggcct
    21481 ggaagaggtc caccgcccgg aaagatggct tgttctggct gacgcacagg gaatcgggcg
    21541 tgaactgaga gaccgttttc actccagaaa ggacgattgc gtactcgtgt ttcggggggg
    21601 cgaatacgaa gctctatccc aacaggaata caggattgat ccggccgaac cggaacactt
    21661 ccgacgcctg ttccatgcgg tgcaagggga gctggacgga gtcgtgcacc tgtggagcct
    21721 ggatgtgccg cttctttcgg cggcgaccgc ccctcacctc gatgataccg ggcacactct
    21781 cgatcgggct cttctgctcg gctgcgccgc caccctctca ttgatccagg caatgaccga
    21841 tgcttacatc ggtccccctt cattgtggct ggtcacccga ggcgctcagc ccgcgggacg
    21901 ggaatgtacc gtccccggat tgtttcaatc ccctttgtgg ggaatgggga aagtgatacc
    21961 cctggaacat cccgacctga gctgcgtgcg gatagacctg gacccggagg gggctgagcc
    22021 cgtggatcat ctgttcagag agctaaaccg gaaggaagcc agagaagacc aggtggcttt
    22081 tcgaaacggg cagcggtatg tggcgcggct gtcgctccac aatgccgtta tcggatcgag
    22141 cccttgctcc gagcctgcgt ccggctcgcg catacccctc actttttcgg cagatggcag
    22201 ctatatggtc accggcggcc tgggaggact gggcttattg acggcccgcc ggctcgttga
    22261 acgtggagcg cgcaacgtta tcctggtggg acgcaacccg cccggcccgg aagcggccgg
    22321 ccggataacg gagatggaaa ggaggggtgc ccgggtgacg gtaatcaagg cagacatatc
    22381 cgatgcaaag caggtcgccg agatcctgga tgatatcgaa gccgcacttc ctccgctgcg
    22441 gggaatcatt cacgccgcgg gcctgcttga cgacggtatc cttgtcaacc tgacatggga
    22501 gcgcttttca cgggtcatgg ctccaaaggt caacggggct tgggcattgc acgccctgac
    22561 tgcctcgcgc aacgtttccc tggatttctt cgtactgttc tcttcggccg cggcgatgct
    22621 gggcaatgcc ggtcaagcca atcatgccgc cgcaaacgct ttcctggatg cactggctca
    22681 ccaccgccgg gcgcaggggc tggcaggact ctccatcaat tggggtgcat ggtctgaaac
    22741 aggtattgtg gctggagacc agagagccgc caaggcgatg aagaacgcag gactgggcgg
    22801 cataacctcc cgggagggtt tgattattct ggaacagcta atggcctgtt ctcctgccca
    22861 gatcggagta tcacccatgg attggcccag gttcatcaag agatttcaat tgcagggcac
    22921 ggccttcttt gaccattttc ccgatccggg cggaaagagt ccgcagcggc agacacaatg
    22981 atattacgag gtcatgattg atgtcgcaat cgaggatcgc catcaccggc atggcagtca
    23041 ataccccact gggggacaaa ctggacgagt ttctgaacaa cctgcttcaa ggacgttcgg
    23101 ccattacccg ctggaaattc cccgattcgg gcaagatcta ctcaaaagtc ggaggagatc
    23161 tgtccgatta cgatatcgcg ggcaaggtgt catcgctgcg ggaactgata ccggaggctg
    23221 tcgctcaacg tttggacaaa ctggtgagct actccccgtg gtctacaaag ctgtccgttt
    23281 tattggccgc ggatgccttc ctggatgccg gcctcttcac cggcctcgct gatcccgagc
    23341 gcatcgcggc cattatcgcc ggccacaacc tcaacggtat gtatatgtac aacagttgcc
    23401 gggaattcgg agaggagccc gactttatcg acaggatgct cgccgtcagt tgccatgatt
    23461 cggatcacgc cgggtgcgtg gcgacaggac ttgacatccg gggtccggtc tacaccgtag
    23521 gaggcgcctg cgcatccggc aacattgctc tgcgctccgc catggacgag attcgacatc
    23581 acggcatgaa cgtggcactg gttgtgggcg ccgtctatga ctgctgcccc ctggtactgc
    23641 acagtcaggc gatgatggga gccgtttcgg catcgagctt caacgaccgt ccgaccatgg
    23701 ccagccgccc cttcgacctc gcccgcgagg ggttcgtacc ctcgcatggc ggcggagcgc
    23761 tggttgtaga ggcatgggag cacgccctgg agcggggagc caggatttat ggcgaactgg
    23821 ttggggtcga ggtgagctgc gcggctacgc gagggccgcg gccttccgag gagcacgagg
    23881 cgcgtgtgat ggagcaggtg ctgcgcagca cgggcactga tccgcaccag gttgatttcg
    23941 tttcggccca cgccacctcc actcaactgg gagacctggc tgagatcggc gccatccggc
    24001 gggtattcgg ggatcacgca agggctctca agatcaacgc accaaagtcg atgctcggac
    24061 acacactcat gccttcggcc ttggtcgagg ccgtggccgc ggttctacag atgagggcgg
    24121 gacggctcca tccctcgatc aatatcgacc ggctcgaccc cgctgccatg gacctggatg
    24181 tttgcgccaa ccgggtggtc gaacacgcgg tgcggtacct gatgaaaccc gcgttcgggg
    24241 ttgggggctt caattcggcg agcatcatac ggtgctacga cgagatgatc cattggaggt
    24301 aattccatcg cagccggtga agccgtatcg ttggccgcct gttgcagaag aaaggagaat
    24361 ggagacatga cgcgagacga tgtgatggcg atgttgatca ggcatacgtg tgagattgtt
    24421 ccggaattgc gaaaggagca cctggatccc gacaagtctt atcgagaaat gggaattgac
    24481 agtctggccc tgatggcgat tctgaccgcg gcgacgagag agctgaaagt gaagatacca
    24541 cgagacaagc tggggaatat caacagcttg aacggactgg ccgatttgtt cgttcaggtg
    24601 gctaaccagc ctgaagcata gcctggttcc catccggaaa cctctatctg tggaagcggc
    24661 tcagctcttt ccgtaggagt gttttttgat gtcccggcga atgaattcca ggatgtccct
    24721 ggcccgcctg gagaaaagcc ttatggaatc gaacttggga agcgtgacga gcttttgcat
    24781 gagcatcccg tccgtatcga accatctcgg aaggaagccg ccgaagaaat aacccttttc
    24841 cctaagaacg gacgcggccc agccggacca ggcctccccc aggttgacat aaacctgaac
    24901 aacctggatt tcccggcttt gcgcctgctt ttcagccgtc gccaacatct cggggaagtt
    24961 ttcccccatg caagaggcgc cgaatcgagc caccctggcg gaatcgaaaa actgggtctg
    25021 aagctgcgtg gagaggcccg acggaatcct ggtcccggag gccacgacat tcctcgacag
    25081 gttcatgccg gacaaaataa agcctagatg gatgatccgc aatgtcttca gcaaggcctt
    25141 agcggcgatc ccggaagggg gttgctgcat agtgatcagc cgcagcgagc cggaagccga
    25201 atttgcgcgc atcgcaagct atgggcgcat gcaccacgtc ggctgggatg gactgcgctt
    25261 taccctcgag gaaacgaaac aactggcacg gcagttcgcc cgcgccgaga tgagcgacac
    25321 ccggatcgaa cagatccacc ggaaaaccga cggctgggtc gccggcctga ttatggccat
    25381 ggaagccgca cgcaatggaa acctctccga ggcgttcatg gagaggcttt ccggtgagag
    25441 tttttttgat tattttacag ccgaaatctt cgaaagactg gacccttcga tccgctcctt
    25501 ccttcttgcc accgcctttc tgccggaggt caccatcggg tcgtccgaga gactgaccgg
    25561 ctcccgaaac gccggccgga tcctgtccga tctgcaccgg aacaatttct tcatctggca
    25621 gagtgattcc gagccaaggg tttacaccta tcatcccttg tttcaagact tccttcgtac
    25681 ctacgccgga agaacccttg aagccgaaac tctcgaccgg atccgggcca gggccgcaca
    25741 aatcatggaa gagcagggac ggatcgaaga ggcgatcgat ctcttcatcg aacagaaaga
    25801 ctggaagagt gcactccgat tgctcctcac ccatgcccgg gccgccttga tccacggcag
    25861 gaacggggct cttgaaaagc ggcttgcccg cctgcctgcg gccgtcctgg agtccacccc
    25921 gtatctttcg tactggcagg tgatgtgccg ccttccggag gattccaggg agagccgtta
    25981 ccattttgaa gatgcatttc gccgattcga gaccgaagga gatacggtcg gcgcccttct
    26041 ggcctggtcc ggagtggtgg agacccttat tttggagctg ggcgatatgc ggcggctgga
    26101 tccatggatc cagtggttcg atgaccgatc cggcgacgaa gcactctttt cttcccctga
    26161 aatacggatg atggcggcaa gaagcatgct gggcgtgatt gcgctgcggc ggcccgacca
    26221 cccgcgggcc gagtactggg tctccgaagc ggaagccctt ctggacagcg gcctcgagat
    26281 gaacagctgc gtccttctgg ccgcccgcct ttactactat tacagcctca tggaagagaa
    26341 ttcccggacc aatctgatct tcaagagact ggagcccttg atcgatccca accggcttca
    26401 gttgatgacc cgcctctggt ggcagtttct cgtgctccac cactcaagat acgccgcctg
    26461 gcagacccgt gaatcgtgcg aagcctattt cgaaaaagtg caccaggcca tgtctctcac
    26521 cagggatctg ggatttcgta ctttcgaccg gttcaacccg tccttcggag cccatgtcgc
    26581 cctggtggcg cgggatttca aaaccttcga cgaaatggtg ggggtgctca gagaatcgct
    26641 ggatgattcc aatatcctgg gccacatcct ctaccaggtc tatctcgggc tcaaattcgt
    26701 ttatcaggga gactatgcct cggccaggga acagggaaag atcgccctgc ggctggccga
    26761 aaacatggga agccccctgt acgagtaccg gggccatatc ctgctcttcc attcctatat
    26821 cggactggaa gcaccggagg cggcaaaaga gcatttttcc catatccgcc gcgtagccga
    26881 atccatcccc catcttcggc atgatgcgat tctcatgctg acggaagctt acctgctgta
    26941 caaaaccggg gacatcccga aaggagcgcg actcctcgaa gccggcctca aaaaggcaaa
    27001 gcggcacaac accatccatt cgaccttctg gtttccttcg gtattgaccg atctgtgtgc
    27061 gtatggactc gatggcgaca tcgaagtgag atacgtgcag gatattatcc gccgcacaag
    27121 gcttatgccc gagtcgtccg agaccgtttc ggagaattgg ccctttgccg tcaagattca
    27181 tgcgttgggc cggtttgaaa tcgaaaagga gggaaaaccc ctggatttta caggacgcgt
    27241 cccccagaag cccctggccc tcttgaaaac gatcattgca ttcggaaggc tggaggtttc
    27301 aatggagcaa atagccgatg cgatctggcc cgatgccgaa ggcgatgcgg cccacagcgc
    27361 cttcaccacc accctcagcc gcctgcgcaa gcttctcggc tgcgaggagg ccgtgcgggt
    27421 gcggcacggc aaggtctctc tggacccgca gctttgttgg gtcgatgtct ggcgtttcct
    27481 ccacatcctg gatgaactgg atagagtcta ccggcagtgc gactgtgaga gccgtcggca
    27541 agacaggccc atgagcgatg cccttctcct ggatcgcctg attcacctca ccgaaagcgg
    27601 gctcgccctc tacaaaggtc ccttcctcgg ggacgaaccc cggcacgccc atgctctggt
    27661 catgcgagaa cgtcttcgca ctcgccacat tcgattgatc ttgaattccg gaaaacatct
    27721 ggaaaaagcc ggccaatggc gccgtgccgc ggacctttat cttcgagggc tcgaaaccga
    27781 cgccctggtg gaggaattct acgcccactt gatgatctgt tatcaccgga tggggctgaa
    27841 agcggacgcc gccgccgttt tcaaacgctg cgagaccacc ctcatggagg cattcgacat
    27901 ggagccctcc gatcagaccc ggcggatata cgaatccctc catcaccgca gttaggccaa
    27961 tacggtgctt ccggtttcgg gcttcctctc gaattctgcc tacacctcgg gcactccatg
    28021 acaatcacgt gttcctctat aaggggaagc cgatcagcga cattcgaacc ggcttgaccg
    28081 atgcttgtga ggccgcaggg ataccgtacg gaaggttcac gaaggacggc ttcatttttc
    28141 atgagcgtca cacgttcgat acctccatga gaagggctgg cgttccagaa tcggttataa
    28201 tagagattac ggggcactca acccgggaga tgttcgacag gtacaacacc gttgaccacg
    28261 acgacaagag gcaggccatt gaccaaatgg gggtatctgc ggaatcttga ccaaactctt
    28321 gaccaagggt cgtttttgaa aagaaaatag ggttaggcga actggcctaa ccctatgatt
    28381 tcttaggaag tgccgaagcc gggaatcgag cacgaccagg ggctctcgaa tggtctcgac
    28441 aatgccccgg gtgtattcga ccgcctcccg cagggcctct tcagttttcc tccctcgggc
    28501 ttcggcagct ctgagagcct tgacttccct gcgcaattgc atcaactcct catggagatg
    28561 cttctcgcct ccgtcctttt ctcggctagc tcttttccga tctcgatgat gcgctatcct
    28621 tttttccatc gcaaggctcc cctcatccga tcgggtcgtc caaacacaac ctgactcggc
    28681 gatgcgttta gcgccttatt ctccggaact tttccttcag gtcgtccatg gcctcctcaa
    28741 gcctgtcctt catggtctcc catgcctccc cactggctgc aagaaggttc gagagcctgt
    28801 acttcaattc tcccagcttg tcttccagtc catccagttc ctcacggtac ttcttcctga
    28861 gctcctgact cttttctccg gcctttgccc tgagctcggc gagcctggaa gtccactcgg
    28921 cgatctcgga ttcgatcctt ttcctgtagc tctcatgggt ctcgggcagg gcttccccct
    28981 ttactttgat gattttgggc tttctgcttt cactcatttt gaaggtgacc gtaaggaccc
    29041 catctttaag ctcagcccgt acatcttcag gactggcgtc cgatggaagg ggaatctcac
    29101 ggtagaatcc gccagtcacc ctttcccgat agtggcagca ctcgtctctg ctctcatccg
    29161 tggattttcg ctcgccacgg atcacgaggc tgtcgcccgc gaccgagaca tcgatgtcct
    29221 tcgccgccac accgggcaac tccgcacgaa tatggtagct gtgttcatcc tggctgaaat
    29281 ccaccctggg agaggcggct tcgagtagtg cgtagtcctg gatcattccc tcgatgagac
    29341 gatccagctg ttcgtgccac tcattgattt ccctcaagac aggcggtttt ttagtccagt
    29401 cctggaatcc tccggcaatc atagacatca ctcctttcct ttgagatccc gtgtaccggg
    29461 agcattgtcc gttgcaccca aaatgggaaa agaccgagag gaggaatctc ctcccgtcgg
    29521 cctttccaag accaccttta ccggcttatt gaaaaatccg gtttatgacg actttacctt
    29581 tatcttgcga ggcttcagaa tatcggcctt ggggagcacc accttgagca ctccgtcttt
    29641 catcatggct tcgatcttct caagctcgga acccatgata gtcagggcgc agaaaatgtc
    29701 aagcaattag tctcacaatc taaatgaatg agatgctgca tggaagcgcc atgcggcaca
    29761 aacaagccgg tcaaacaatt tgaagcaagg aaaaaatgcg cctggctatg aaactacgcc
    29821 cttgcaatgt ggtggatcag gtgctatttc ttcccttgtc tcgctctcgt ataacgtctc
    29881 ataaagcaaa tgctgaggag ctccgcaacc atgaaggaga tgagccttgg gaagaatggt
    29941 cggatatgcg cgaatgtcgc cgctggaaaa ggatcttcag ccgcaggtga atgcgctgaa
    30001 agcagccggg gttgaggaaa ggcatctatt tacggatgag accatcgagc cgagggcgga
    30061 gtggagcggg ctccatgcct gccttggtga gctgatctca ggggacaccc tgatcgtttg
    30121 gaaaatcgac agaatcggaa gatccgtccc ccacctccta gagcttatgg agaacctgca
    30181 tggacgaggt gtcggcttca gatcgctctg cgacctgacg atcgacacca cgggtgtttt
    30241 cggggatgtc gtgacggagg tgatttcctg cctggcccgg ttctcccagc gggtgctcca
    30301 ggagcgtgca agagcagggg tcgagacggg ccgcgccagg ggaagacagg ggggaaggaa
    30361 acccattgca tcgagtaatc cccgggtgag ggctgcaaag gccatgagcg tcaatggaaa
    30421 tatcccggtg agggaaatct gctccaccct tcggatatcg cgggccacct actacaggta
    30481 tcttgacctt gaggaagaat gaacgcggcc cacgccctat tcgcttacct cgcaactgaa
    30541 ggccaatctt tgatgaattc gcaagaacct tttgtgaggg gcatccattg ggacgtgatg
    30601 gatgtctaat gcctgacgcg aaaccggttt ccaacccaat aaacttcgga tattgtcggg
    30661 atcgccgctc ggaggaagcc gtccattgtt caggcgcaag aggcgagaat ggaccctctc
    30721 gagatattgt catacccggt tgcgcccaag gtcgccctga caggctgagg gaagatttcg
    30781 agcaaagggc tgagtcctgc ggatgggtag tttcggcttg aatgcaactt cgtatcaaac
    30841 gttgtgtacg gccaaaccga atggagaacc ccgggtcgac gatgatggaa cggggtaccg
    30901 gagagaaaga gtcggccccg ggcttggcct cgggggagct tgaaccggaa tccgttccct
    30961 tggaggttcg cccgggggtg cccggcttga agttttccgg caatcatcgg aatggaaacg
    31021 ggagaagggg aatgacgttg aggagaaggg gcctgttgat cctcttcctg gtgacggcct
    31081 gtgtgggggc ggaccaggcg gccaaggagt gggcccgggt gcgtttgtcc ggaagttccg
    31141 ccctgtcctt ggcgggaggg gttctgaagc tggactacca cgaaaaccag ggaggagttt
    31201 tcagcttcga atgactgctt cccgagcgat ggcgcgggaa cgccgtcacc atcgggtcct
    31261 cggtattttt ggggttgctt cttctctttc ttctggttcc cacgagatcc tcaaccctcg
    31321 gagtctgggg g
//
LOCUS       DAOOVC010000032        31193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_980_length_31193_cov_5.716713, whole genome shotgun sequence.
ACCESSION   DAOOVC010000032 DAOOVC010000000
VERSION     DAOOVC010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 31193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31193
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_980_length_31193_cov_5.716713"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLM79_06390"
     CDS             <1..426
                     /locus_tag="PLM79_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV85973.1"
                     /translation="YLVDVRSKSEYTGASSGYSYLEAKGRIPTAIWAYDADDSSAVYN
                     DADGTLRSYPEVLEVWKQRGIQSTGAANKFDKKAIFYCGGGYRSSLTFLYAFMMGYSN
                     IRNYSSGWADWSTTYTKDPSCGGITPGWCQEPSGRPIVP"
     gene            complement(479..1945)
                     /locus_tag="PLM79_06395"
     CDS             complement(479..1945)
                     /locus_tag="PLM79_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV85974.1"
                     /translation="MSTWQIEIWAGLHYVALAGLCLYGIHRLWLLACWFMECKRPPHA
                     PRRLPSPRNLPRVTIQLPLYNERFVAARLVDAVARMDWPKDRLEIQVLDDSTDDTRAI
                     VDERVIHWRNKGIRIRVIRRRDRKGYKAGALEHGLRQARGEFIAIFDADFLPPQGFLK
                     RTIPFFSGEKIGMVQARWDFLNAEYSWLSSIQSLLLRPHFSIEHGVRFRRGLFFNFNG
                     TAGIWRRKAIETAGGWQSDTVTEDLDLSYRAQLRGWRFVYLDDLAVPSELPVTMASFR
                     IQQQRWAKGSIQTAKKILPQLLVSPVRWSVKMEATAHLLANLGWLLGTVVFLTLYPAI
                     LWRVGIGTYQILRIDLPLILCTSLAMLLYFFIHALRSRPAIPFYHLLLLPILGIGIAP
                     CLALSVLRGFFSRGGIFERTPKFGIRGRDRLPRFAFLYQQQRVFHLILNAVLFTYGLL
                     PILFAWQRETWPAVPFLALFPLGFFSVLILDLKEMVKQ"
     gene            2244..3083
                     /locus_tag="PLM79_06400"
     CDS             2244..3083
                     /locus_tag="PLM79_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85975.1"
                     /translation="MRSPLKFVICIAVGIITAFFLSVCQCHAGAWTLPQGKLYERIAV
                     NYYFADSEFSTQQDNKGFKKFSDLNVSNYLEYGITDRITLINAMYYKFLEKQALREEL
                     DSDGFGDVDLGLKGKLAEGSWGVLSTQGLVKIPGAYGKNDALPLGNGQYDLDMRVLFG
                     RSLWPYLPGYCNFEIGYRVRLEDPSDELRYLVEFGVDFTKELYGRVKLDGILSMDNGS
                     SVSTSGNPTATNNFDLGKLEMAVGFKLTKTWGLEFCIVPEIYGRNTTAGTTYTLALTY
                     QTR"
     gene            complement(3536..5185)
                     /locus_tag="PLM79_06405"
     CDS             complement(3536..5185)
                     /locus_tag="PLM79_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85976.1"
                     /translation="MVSARTRRVGPFEKIAFRQILSWGLVSILLALYSLLALQSDMAS
                     AVPALVAVSAVLVSLLALVLYAEGRGAVRWSPTGILLVAAGLRVLFVFGPPELSDDLY
                     RYLWDGLQLANGHNPYRAAPSEMPPGDEASRSLLGLINHPDFITIYPPAAQLIFAAGA
                     YLGEAFWGLKILLLTMDLFTCFLILRLLTVLGLSPGRSILYAWHPLPILEIGSSGHID
                     GAGLFFLFLSLALLAVPLGRDAVRTASSPWFGRCRMLALRTFLAGLAFATAVLVKLFP
                     LFFLPGLLMLVGKGHRSSFLVGVLTGASALVFPFMPEIGNLLGTLDFYLQNWEFAGLV
                     FRVLRRTTLSGNSARLILASLFLVSLAAVYGTLRRGKTAVVEADPGERRQPPRVGALP
                     DPTAGSGVLPAHPPKEPSREPECRQDPASVKLFRIPDPAATAKGPGCVTDLPLCGVLP
                     RVLQAFSVVTVAFLFLTPTFHPWYALYLVCFLPFTLGTARLVLSWGVFLSYYVLILYK
                     PHGLWLENDWVPLWIWLAPACAFLLAVLFQRSRKGDETACP"
     gene            5447..7318
                     /locus_tag="PLM79_06410"
     CDS             5447..7318
                     /locus_tag="PLM79_06410"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOV85977.1"
                     /translation="MCALKTRSFFPDGDSREHLPGDPAGGDDSRGETDLALLLKAVSA
                     NCIECGLCRKDCQFLRNTGNPKSVADAFDPADKAEHSKPFECSLCGLCTSICPAEVDP
                     AALFLEMRKEAVRSADWNCPEHSTLLDYERRGTSRRYTWYALPEGCRTVFFPGCTLPG
                     TRPDRVKLLFDHLRTTIPDLGIVLDCCAKPSHDLGREAFFQSTFGEMRDYLLENGVQN
                     VLVACPNCHKVFKNHGRTLVTKTVYEVLADNGLPESSKISGSVTVHDPCAVRFEEPVH
                     KAARDLVGRTGLTVIEMPHHGRETLCCGEGGAVGFLSPELAKNWRAMRKQEAQGHRII
                     TYCAGCAGSLGQVAPTSHILDLVFEPEAVMAGHVKVSRAPVTYWNRIRLKNWFKKHLD
                     AAVTRERTFTTERSSGKGGLALRITVLALLVSAIVAVRTTGATRFLEQEVLRAWIQSC
                     GALAPLLYMLVYSVAPSLFLPGLPITIVGGVLFGPFWGVIYTITGATIGACLAFLISR
                     YLARDWIAAKLTSPRWRRLDEEVQRHGWKVVALTRLIPLFPFNLLNYAFGLTKIKFLH
                     YGVTTFVCMLPACIAFILFSSSLLDLIRGRISLEFVIGLLLVGLVSLIPVLYRRRRG"
     gene            complement(7350..8243)
                     /locus_tag="PLM79_06415"
     CDS             complement(7350..8243)
                     /locus_tag="PLM79_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV85978.1"
                     /translation="MTAAISKLIRGSAKFRVGSLFLFMLWGGVVTHAFGASTAPDLAL
                     IDVSELHENRQAWVILDARPRCRWEAGHVAGAYPFSWEDHTTTDEQGVPFRLRPAGEL
                     ADALARMGIDEKTPVAVYGDAEESWGGEGWACWVLSWLGHKGPIRLVAGGIQAWLARG
                     YYLSTETDRGESQGIRYRVSLRPHLDIRTSEVQERAADLALIDTRSSVDWVLGHLPGA
                     IHIPWTDFYSGKDRRPLDAAALRKLFVDHGVDTDKPIVYYCAGGVRSGYAWTVHTICG
                     LPPAHNYVAGMEEWVRCAKGR"
     gene            complement(8319..9056)
                     /locus_tag="PLM79_06420"
     CDS             complement(8319..9056)
                     /locus_tag="PLM79_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV85979.1"
                     /translation="MYEGKTIALVIPARNEALTLPRVLGSVPHEVDRVLVVDNGSTDE
                     TSRVARANGAQVVNEPTAGYGRACLAALEVLEQEPPEIVAFADADGSDDLSHLPELVA
                     LIAGGKADLALARRSPVEAHALSLQQRFGNWLATRLILLVWGHGYADLGPMRAITWRA
                     LQGLGMEDRDYGWTIEMQVRALRMGLRVIEHPVPYLRRKAGRSKVSGSLSGVLRAGTK
                     ILWVIAREFVGEKSCVVGGRKPRPKTP"
     gene            9721..11505
                     /gene="typA"
                     /locus_tag="PLM79_06425"
     CDS             9721..11505
                     /gene="typA"
                     /locus_tag="PLM79_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOV85980.1"
                     /translation="MKTRTDLRNIAIIAHVDHGKTTLVDAMLWQSGIFRDNEPVAERV
                     MDSLDLEREKGITIMAKNTAVVYRGVKINIVDTPGHADFGGEVERTLNMVDGVMLLVD
                     ATEGPLPQTRFVLGKALARGLPPIVVINKIDRPDRRIGEVLDEVFDLFIDLDATEEQL
                     DFPVLYTNAKAGVALTEPDGRGRDLVPLFEAILRSIPSPSCDPGAPLQFLVTTLQHSD
                     YVGRIAVGKVVNGTLHCGQEVTLLKAGIPLRKTHLSALYAYEGLNRVERRTVEAGDIA
                     AVAGVEDVFIGDTLGDPQDPRPLPVITVEDPTLSMVFSVNTSPLAGKEGRFVTSRHIK
                     ERLDKELLYNVSLRVEPAENRDSYRVSARGELQLAVLVESMRREGFELSLSKPKIITR
                     TVHGQLVEPKELAVVDVPEKFLGIVTEMLSSRKGRMTKMVNNGFGRVRLEFELPSRGL
                     IGFRSQFLTDTRGTGILNTLVIGHTPYAGEIVGRPNGVLVSDRDGKAVTYAIHHLQPR
                     GTIFVNPGDPVYAGMIVGENSRSDDIRVNITKEKKLTNIRASGSDEALRLIPPRQFTL
                     EQAMEYIGEDELVEVTPQSIRLRKMKLR"
     gene            complement(11744..13171)
                     /locus_tag="PLM79_06430"
     CDS             complement(11744..13171)
                     /locus_tag="PLM79_06430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOV85981.1"
                     /translation="MNWLIAAPEISYFASALWFLVLSLAAKPDARREHRTSLLLGGLG
                     VAICTVSIGQEGYLFTRAFRVDLFSQVFKELLSLSLFLVLTLCGKMRDLRERHRHDFY
                     VLLFVCTLAMMLLVSADHLLALFLSLELSSYSLYILVAVRRDQRFGTEAGIKYFLVGT
                     FASAVMLFGLALLYASAPATYLSDMARAPSGMAERPLAFLGLLMTLGGLFFKLAVFPF
                     HFWAPDTYQGAMNQVAAYIATASKVAAVAVMVRVVALAGQGSAHLVQILAVLSIMSMT
                     VGNLAAIAQKDLKRLLAFSTTAHAGYVLIGILAVNESGYAGAVFYAMALLLMKFSAFL
                     VVTVVAGDGKNLEVEDLAGLHRRSPILALTLMVSLFGLAGIPPTIGFTAKFLVFVAAM
                     ERGHFALVLIAMINVVVSLYYYLLVVKAAYLTEPREETGPRAVSLSTRILAGALASGM
                     VVAGFFPTYLMELADSAVQVLLSGP"
     gene            complement(13168..14649)
                     /locus_tag="PLM79_06435"
     CDS             complement(13168..14649)
                     /locus_tag="PLM79_06435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOV85982.1"
                     /translation="MHLTYADFPYLSAILFSCVAGLGVILAVPDERKDLIRKAGAVFS
                     GATLFLSVSLFVSYDRHLGGLQFVEKIAWIPSLGIHYFNGVDGFSLPNLLLTGIVFFT
                     GVLTMWGMESRVKEFYALYFVLVTGVFGLFMSMDLFFIFVWYDVSLFPMYLLIAVWGS
                     TRKEYGAMKLTLYLLAGSALILPTVIFLFVRSGLNTFDVVSLMQSGVFTPQDQKFAFL
                     LLYLGFGILAGVWPFHTWSPVGHVAAPHAVSMVHAGVLMKIGAFGVLRVAVMLCPEGW
                     KQWADLMALLAACGILYGALVGLRQTDLKYVIGYSSVSHMGVVGLGLSTVTLEGLDGA
                     VFQMFAHGVMTALLFSSVGYIYDRTHTKMIPELGGLSRVMPVASAYFIVAAMAGMGVP
                     GLACFWGELVVFIAAFKVYPVRGTTAVLALVVSALFMLRVVQHTFYGPPKEEYAHLPD
                     ISLVQGIPRMILLAVLLLFGLYPALLFDVIQTASVPFMVGLSR"
     gene            complement(14672..16615)
                     /locus_tag="PLM79_06440"
     CDS             complement(14672..16615)
                     /locus_tag="PLM79_06440"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOV85983.1"
                     /translation="MTGFSAETLTLPTVALATLLPFAAFVVILLFTRSRPRLSAAVAV
                     GVSSVSLAGTLVLLTLHWNMEKPVQHTARWLVCCTGFMLHTNHRAVCCTGYLLDPVSL
                     LMLAVVAAVSFLIQVYSLGYMAGDPGFSRYFAFMSLFVWAMFNLVVSPTMFQLYIFWE
                     LVGLSSYLLIGFWYEKLMASQAGKKAFVMTRLGDAAMFLGILLVLLHLGNLDILEMDS
                     PKAAASMSPELLTLSALLIFGGIVGKSAQFPLLTWLPDAMQGPTPVSALLHSSTMVAA
                     GIYLYERLYPFFLFSRTAMTVGLAVGTVSMLLASTMAMVNRDIKQIWAYSTISQLGFM
                     LMGLDAGSLFSGAFHLTTHAAFKALLFLCSGVFIHRYATNDIFELGRQGCRSLKIPMA
                     CLIIAAGALSGLPLLSGFFSKEVILGSLAELSNPLWLGAGLLGVFLTSYYAFRLVFIL
                     LFPGESGPGHGEAPEASAPSHGSAHSRFHEWILDGPLIVLAGVTLLLGLFQVPLEQFL
                     RGGEISGLHWPDPLHHPVLPYVSVALAALGLGLSWVEFGRRGAPRVGFVERLPGLFRL
                     FSQRWYLDHVYGFLVDRGIDRGISRLLYLNDHKVIDGFVDGTAESTAAGGRITAFLHT
                     GMIQYRLLTTFAVVVFLALYFFL"
     gene            complement(16656..16958)
                     /gene="nuoK"
                     /locus_tag="PLM79_06445"
     CDS             complement(16656..16958)
                     /gene="nuoK"
                     /locus_tag="PLM79_06445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOV85984.1"
                     /translation="MIVPFSHILLLAGVLFSLGMFCALARRNLIMIVLGIEIMLNGAS
                     IAFVGSALHWQQLEGQAFVLFILAVAAAEVSLGLAILLHAFRRTGSFDPEAYNSLK"
     gene            complement(16955..17473)
                     /locus_tag="PLM79_06450"
     CDS             complement(16955..17473)
                     /locus_tag="PLM79_06450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOV85985.1"
                     /translation="MTLYGFLFYVLAAVVTASTAVAVTRRNLVHAVVYLIFSYFGTAM
                     LFYLLGAPLLAALEVIIYAGAVMVLFLFVVMMIRVEASGEHFFPLSQWIPAALFSLVY
                     LAAGSVLMLAAPGTEIPLKMLTAAPRDFGLYVLQNHWFFIEVISLLLLVALIGALQLG
                     RKQEGLSREEEG"
     gene            complement(17510..18046)
                     /gene="nuoI"
                     /locus_tag="PLM79_06455"
     CDS             complement(17510..18046)
                     /gene="nuoI"
                     /locus_tag="PLM79_06455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOV85986.1"
                     /translation="MESAWDAVKALASGFAETFVHLFRKPITEEYPDYKRPLFARTRA
                     RIVLTRDPDGRERCVACYLCSAVCPVSCISMESAEKPDGRRYAPWFRINFARCIYCGL
                     CEEACPTSAIQLTPDFEFCKTEVLDFVYEKEDLLVNHCGKDPEYNFYRVAGIATDAGG
                     KGEHFREKEPVNVKSNLP"
     assembly_gap    18072..18171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18172..19116)
                     /gene="nuoH"
                     /locus_tag="PLM79_06460"
     CDS             complement(<18172..19116)
                     /gene="nuoH"
                     /locus_tag="PLM79_06460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOV85987.1"
                     /translation="MLAWMAGFFILIVKLGAVLVLLLLMAAYLVWLERKFLARLQIRY
                     GPNRAGKYGLLQPVADLVKMLTKEDTVPQGADRPFFLLAPAVVATTAFLMFAVVPLGK
                     DLTFWGRSVPLVVTDLNIGLLFVFALSSLGVYGVALGGWASNSKYSLLGGIRGAAQMI
                     SYELSLGLSLVPIVMMAGSFSMVDIVRAQESYPFVLVQPVAFVVFLLSAMAESKRIPF
                     DLPEAENELIAGYHTEYSGMRFGLFFLGEYVNMMVLGGIVAVLFLGGWRGPVLPPFVW
                     LLVKILGVAIFMIWVRGTLPRLRYDQLMALGWKVLIPVS"
     gene            complement(19358..21079)
                     /gene="nuoD"
                     /locus_tag="PLM79_06465"
     CDS             complement(19358..21079)
                     /gene="nuoD"
                     /locus_tag="PLM79_06465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HOV85988.1"
                     /translation="MAAFEVDESRIREVLAFLKKESSPRFQRLDDLTAVDESARRDRT
                     WDPGYASAIADVGAAGHYAQRLPKPYPDFTLTYHLLSLDTPVRLRLKVGLNGDSPAAP
                     SVTGIWPSANWYEREVFDLFGIRFEGHPNLRRILLPHNWEGHPLRKSYPGRATQMPPY
                     TREDARREQPLDAEVLMRLDGKFEDGTHEFLLNIGPHHVSTHGLLRCIVSLEGEEITG
                     LDLDIGYHHRGPEKIAERQTWHQFIPYCDRVDYLAGAANDLPYVLAVETLADIRVPER
                     AQVIRVLLSELFRLSNHLVWFATYAHDVGAMTPNFYAFAEREMILDIVERITGGRLHP
                     SWFRIGGVAGDLPEGWKEAVDDFVRIFPRRLEEYERLISKNPIFKARTRGIGRISAEE
                     AVDWGVTGPNLRACGVEWDLRKKFPYSGYERFQFEVPTAAGGDCYARYLVRVEEMRQS
                     LGIIEQASRNMPPGRTITDDYRYVVPKRDEMLRDIESLIHHFVNVTRGPKIPRGEAYV
                     SCEIPRGEQGYYVVSDGLGHPYRLRIRAPGFANVQAMPILAEGESIADLIAIIGSLDF
                     ILPDIDR"
     gene            complement(21377..21832)
                     /gene="nuoB"
                     /locus_tag="PLM79_06470"
                     /pseudo
     CDS             complement(21377..21832)
                     /gene="nuoB"
                     /locus_tag="PLM79_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
     gene            complement(21805..22215)
                     /locus_tag="PLM79_06475"
     CDS             complement(21805..22215)
                     /locus_tag="PLM79_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOV85989.1"
                     /translation="MQPIAGEAIPTPWGPGVFTLTVYAVLVFAFVASQLFLAAWLGEK
                     KISAEKLRPYECGIVPTGTARFRYPVPFYLVAVFFLIFDVEGAYIFTWAVIYREAGWP
                     GWIQMAFFIVLLLVGLLYVWKHGGLDWRPGYKAK"
     gene            complement(22459..24582)
                     /locus_tag="PLM79_06480"
     CDS             complement(22459..24582)
                     /locus_tag="PLM79_06480"
                     /inference="COORDINATES: protein motif:HMM:NF023211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85990.1"
                     /translation="MRESHGERSGELELFIRAVLWAVVLAFAVVWGVSAARAGDKGVF
                     LTGLSVVGPDTVPESGSARFKAVAVFSDGTRKRVTGRAAWSENSSFAVISAGVLKTRP
                     VGVEKQLTITAKYSSGGIIKTAKKRVTIVNSAVAKAALTDIRVSGAASVNEGTVSSYT
                     ATASFSDGTQRNVTGEASWDEGSANARFGSPGVLTALEVGGNEEVTLTATYSSGGITR
                     SGVLVVTIVDLDSLPPLSGSHADKFAVFEGTKTCLQCHRDRAVEVYRSEHYQWRGKLG
                     AINDFCIYPDPNHIGRLTNAYGVEVDAGCGKCHVGMGAKPVAAVSPTDAQLENVDCLI
                     CHAPQYKRTVDPVTKARFIPDEEKMGMTILEAAVDIRKTSRDTCLHCHTKSGGGDNFK
                     RGDLEEHHRSPGTRSFDVHMSPASLGGGDLHCTQCHRVSGHKIAGKGADLRVQEGTRP
                     DCTTCHTPTPPHGDSKINAYHLQRVHCTTCHIPSFAKVAPTDVFRDWGAPGELNLATG
                     LYDPARTMKANVTPQYRFWDGQTSTFYDFGTPADAQPNGNVLMAGPVGSVSAGGSKIY
                     PFKVHTALQPMDLETRYLLPLKIGLFYMEGDLVNAVPYGQDAVGWPRNPYDFRQTERW
                     MGLFHEVSPKEDALGYAGSCTTCHGTSTPRMPLLSMGYEAVKPVADLCKTCHESKKYS
                     FGSVHPRHSRKGYDCSECHSFSKAR"
     gene            25230..26018
                     /locus_tag="PLM79_06485"
     CDS             25230..26018
                     /locus_tag="PLM79_06485"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV85991.1"
                     /translation="MGLVLLGIISVIVGVLIGTVGIGGILLIPCLAYIADLSTHESMA
                     TALFSFTFTGLAGTWMFQLRRTIDWGISLPVCMGAVLFSYLGARVNAGVDPRILNVLL
                     SLIILFSGVYTLRSSKGDGFEYLDGRSAAQRLLLLGTGAVVGFGSGLTGTGGPVLSVP
                     VMVLMGFAPLTSIATSQVLQIAVASAGTLGNLQNGSVHLGLGLSVTVLELAGVVLGVR
                     LAHSLATVRLRQCVGAVCVGVGLLILFRSREILFIGWEHVLRRI"
     gene            26141..27112
                     /locus_tag="PLM79_06490"
     CDS             26141..27112
                     /locus_tag="PLM79_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV85992.1"
                     /translation="MNPADRDLVREWGKKLSGPWLLKAVLTDDPRSPKLESFYRALAE
                     TVSSPDFIVERRPGREFPELVVGEVWRFHMVPSGRELEPFLEVISAMADPRPLPLPTH
                     SRELEMPLWSSDLLLFVTPQCPFCPAAVRQVIPLTASPARSRARVVDGALFPEIAEQH
                     GVKSVPTLILNGKFRMTGTITLDEILRILHRSDPSLLDTDTLLRMLKEGQAGLLANMM
                     VEKNTVFEGFLPLLVHPEWNVRLGAMVVVETLAERNPPLAGELLDTVWDSVPGADSRV
                     QGDFLHLIGELGDSRWLSRMEDLARRNPDPEVREVLEETLELLRERP"
     gene            complement(27158..27628)
                     /locus_tag="PLM79_06495"
     CDS             complement(27158..27628)
                     /locus_tag="PLM79_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV85993.1"
                     /translation="MTKPQGSRGTILSCTVFFFLLGTALPASWGAVSSEDKFLVKFVE
                     RNWLNGKAWDKLTYPGKLGYVCGLFDGITLFWAMADAEGVKKSVVDPIFSAAGVPSKY
                     TVGDVVDGMDDFYREPKNLSLPAICAYLHFVYRTRGEGEEAMKKRLSRWHKIFK"
     gene            27901..28422
                     /locus_tag="PLM79_06500"
     CDS             27901..28422
                     /locus_tag="PLM79_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HOV85994.1"
                     /translation="MKRTFILTLVAIIAVVFGTWHPAAAQATPEQAPQVQPRMGCEKN
                     FDRLDANQDGQVTKEEFMAAPHRTRNPERMFNKRDQNGDGTLTKEEFCATRGPGRGMR
                     GGRGCEKNFAKMDANHDGQVTKEEFMAISHRSANPERMFQKRDANGDGALTLDEMCSG
                     PPPGQGRGRAGTQ"
     gene            28876..29628
                     /locus_tag="PLM79_06505"
     CDS             28876..29628
                     /locus_tag="PLM79_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOV85995.1"
                     /translation="MGHVETSEKEGIATVSISRGKVNAINEVVVEQIQEALSRIQANE
                     TIRAVILTGRGRFFSFGFDIPEFMSFSREDFSRYLIKFTDLYARIFLFPKPVIAALNG
                     HTIAGGCMIASACDYRLMVKGKAKISLNEITFGSSVFAGSTAILKYCVGTVNAQEILY
                     SGRMYTAEEALALGLVNTVTSEDDLLIKAEMAAKEFAAKDPVAFKSIKRILRRRAMEE
                     IARFEEESIKEFVEIWYSETMRRNLSRITISP"
     gene            30332..30787
                     /locus_tag="PLM79_06510"
     CDS             30332..30787
                     /locus_tag="PLM79_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOV85996.1"
                     /translation="MADLADRLRHKGDEPHLLREVMRLHHVLLSVFSRQVGMPASRLA
                     LMRFLATRHSEDIGIMEIARCLGVNPAAVTRQVKEMESEGLVDRVADARDGRRSHVKL
                     SEEGLRVFERIHERAHEFERELGSHISSEDMATAVRVLSQLRGDLENLR"
     gene            30804..31142
                     /locus_tag="PLM79_06515"
     CDS             30804..31142
                     /locus_tag="PLM79_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOV85997.1"
                     /translation="MKTTEKRSFNLRGFIVLTATVTGLGLPITGWANHLHRMEPWISS
                     SQHAWMAAHNVLGILFLVSAVAHAVLNRRILFKYIRGHAACRRVGREAVGAALLVAVL
                     MFVAVGHAFH"
BASE COUNT         6684 a         9470 c         9279 g         5660 t
ORIGIN      
        1 taccttgtgg atgttcggag caaaagcgaa tacaccggag cgagcagcgg gtatagctac
       61 ctcgaggcca agggccgcat tcccacggcc atttgggcct atgacgccga tgattcgtcc
      121 gccgtctaca acgacgcgga cgggaccctt cgcagttatc ctgaagtcct cgaggtatgg
      181 aaacaacgag gcatacagtc cacgggagcg gccaacaagt ttgacaagaa agccatcttc
      241 tattgtggcg gcggatatcg ttcctccctc acattccttt atgcgttcat gatgggctac
      301 agcaatatcc ggaactactc gagcggctgg gccgactgga gcaccacgta cacgaaagat
      361 ccctcctgcg gcggcatcac ccccggctgg tgtcaggaac cttccggcag gccgattgtc
      421 ccctaggaaa cgttcaccgc ggtgagggga agacgaccct tcaagggtct tcccctccct
      481 attgcttcac catctccttg aggtccagga tcagcacgga gaagaacccc agcgggaaaa
      541 gcgccaggaa cgggactgcc ggccaggtct ctctctgcca tgcaaaaagg atcggcagaa
      601 gaccataggt gaacagcacg gcattcagaa tcaggtggaa gaccctctgc tgctgataga
      661 ggaacgcgaa acggggcaga cgatctctcc cacgaatccc gaacttgggc gtgcgttcaa
      721 agatcccgcc ccggctaaag aagcctctta ggaccgacag ggcaagacaa ggggcaatgc
      781 cgatgcccag aatggggagc aacaacaggt ggtagaaggg aatcgcgggc ctgcttcgca
      841 gggcatggat gaaaaagtat aggagcatgg cgaggctggt gcagagaatg agcggcaggt
      901 caattcgcag gatctgataa gtgccgatcc ccacccgcca caggatggcc ggatagaggg
      961 tgaggaagac cacggtgccc aagagccagc cgaggtttgc caagagatga gccgtcgctt
     1021 ccattttgac ggaccaccgt accggagaga ccagcagttg cggcagaatc ttctttgccg
     1081 tctggatgga accctttgcc catctctgct gttggattcg aaaagacgcc atggttacgg
     1141 gcagttcgga cgggaccgcc aggtcatcca ggtacacgaa ccgccagccc cggagttgag
     1201 cacggtagct caggtccagg tcttccgtca cggtgtcgga ctgccaccca cccgccgtct
     1261 cgatagcctt tcttcgccag atgcccgcag ttccgttaaa attgaagaac aatcctcttc
     1321 ggaacctgac cccatgctcg atgctgaagt gcggtctcaa gagcagggat tgaatgctgc
     1381 tgagccacga gtattccgcg ttcaggaaat cccaccgcgc ctgcaccatg ccgatcttct
     1441 cccccgaaaa gaaggggatg gtgcgcttca gaaatccttg ggggggaaga aagtccgcat
     1501 cgaaaatggc aatgaattct ccgcgtgcct gtcggagtcc atgctccagg gcaccggcct
     1561 tatacccctt ccgatcccgg cgtcgaatca cgcggatccg aatccctttg tttctccaat
     1621 ggattacacg ctcgtccacg atcgcccgtg tatcatccgt cgagtcgtcc agaacctgga
     1681 tctccaatct atccttcggc caatccatcc gggcaacggc atccacaaga cgcgccgcca
     1741 cgaaccgttc attgtaaaga ggcaactgga tcgtcacccg gggaaggttc cttggggaag
     1801 gcaagcggcg aggagcatgc ggcggcctct tgcactccat gaaccagcag gcaagcagcc
     1861 acagccggtg aattccgtag agacagagtc cagccagtgc cacgtagtgc aaacccgccc
     1921 atatttcgat ctgccatgtg ctcacgaact cattctccat gccgggtatg ccgtagagcc
     1981 cgacgctgtc ggacgttcca ccccctcggg tttccggcaa cgtccccgaa cgattgggtc
     2041 gacgccgcat cgagatgtgg aaggcgtttc tctcgaattg cctctatacc acagaccgcc
     2101 ccgggtttct cattgccatt ttggattcat agattcaaga acatcgaaat taccgattag
     2161 acctcgacgc acgctgattg tagcttgact gacgttgttc atgcgaccac ctgcacttca
     2221 accaccaagg gagggaagtg gttatgcgtt caccgctgaa gttcgtgatt tgcattgccg
     2281 tcggaatcat caccgcattc ttcttatctg tgtgtcaatg ccatgctggg gcatggacat
     2341 tgccacaggg aaagttatac gaacggattg ccgtcaacta ctattttgct gattctgaat
     2401 tcagcacaca gcaagacaat aagggattca agaaattcag tgatctgaat gtgagcaatt
     2461 atctggaata cggaatcaca gacaggatca cccttatcaa cgccatgtat tacaagttcc
     2521 tcgaaaagca ggcgcttcgc gaggagttgg attcggatgg tttcggagat gtcgatttgg
     2581 gactcaaggg aaagctggca gaaggctcat ggggcgtcct gtcaacccag ggcctggtga
     2641 agatccccgg ggcctacggc aagaatgacg cccttccctt gggaaacggc cagtacgatc
     2701 tggacatgcg agtgctcttc ggacgttctc tctggcccta ccttcccggt tactgcaact
     2761 tcgagatcgg gtatcgagta cgcctcgagg acccttcgga cgaactccga taccttgtgg
     2821 aatttggggt ggatttcacc aaggagctat acgggagggt gaagctggac ggcattctga
     2881 gcatggacaa cggcagttcc gtgagcacct ccggcaatcc tacggccacc aacaatttcg
     2941 accttgggaa actggagatg gcggtcggct tcaagctcac caagacatgg ggtttggagt
     3001 tctgcattgt tcccgaaatt tatggaagga acaccactgc gggcacgacc tatactctgg
     3061 ccctcacgta tcagacgagg taatgcgaag aggcgcccaa ggtcgctccg acatgctgag
     3121 tgaagatgtt cggcaaggag ctgaatcctg cgggtgggca gttccggttc gagtgcaacc
     3181 tcgtataaga caccgattca cggccggaat ccgtggagac gcccaggctc ccgcggattc
     3241 gaacgaccgt gcctcgggga atccggatcc gtcccttcgc tcaggcggga atcccatatc
     3301 tccggcccaa caacaccaat gtcggaaccg gtgggaaaac cggcaagagt aagtcaaggt
     3361 cttcaatgag aggggggcct gaggattttc ataggaagtt gcattcaagc tgaaattgtc
     3421 atgccgcagg attcagccct ttactcaaaa tcttcactca gcatgtcagg gcgaccttgg
     3481 gcgcaacccg gtatcaggcc gaagctttcg aaatgggacg aggggtagga ccacatcatg
     3541 gacaggcggt ctcatcgcct ttcctgctcc gttgaaacag tacggccagg agaaaggcgc
     3601 aggccggagc cagccagatc catagcggga cccagtcgtt ttccaaccac aacccgtggg
     3661 gtttgtagag gatcagcaca tagtacgaaa ggaatacccc ccaactgagc accaaccttg
     3721 ccgtccccag ggtgaacggc aagaaacaaa ccaggtacaa agcgtaccag ggatggaagg
     3781 tgggcgtgag aaacagaaaa gccacggtca cgacggaaaa ggcctgtaag acccgaggca
     3841 ggactccaca caacgggaga tcggtcacgc atcccggtcc cttcgccgtt gcggcggggt
     3901 ccggaatgcg aaataacttc acggatgccg ggtcttgccg gcattcgggt tccctggagg
     3961 gttccttggg cggatgtgcc ggcaagacac cggagcctgc tgtgggatcc gggagggccc
     4021 caacacgggg aggttgcctc cgttccccgg ggtcggcctc gaccacggcg gtctttcccc
     4081 gtcggagtgt tccgtagacc gccgctaagg aaaccaggaa aagcgaggca aggatgagcc
     4141 gtgccgagtt ccccgagagc gtggtcctgc gaagaacgcg gaagaccaat ccggcgaact
     4201 cccagttctg gaggtaaaaa tcgagggtcc ccagcaggtt tccgatctcg ggcatgaaag
     4261 ggaagaccag ggcgctcgcc ccggttagaa cccccacgag gaatgaggac ctgtgacctt
     4321 ttccgacgag catgagcaaa cccggcagga agaacagggg gaacagtttc accaacacgg
     4381 ccgtcgcgaa ggcgagaccc gcaaggaagg ttcggagggc cagcatccgg caccgtccaa
     4441 accaaggact ggaggcggtt ctcacggcgt cgcgccctaa agggacggcc agcaatgcca
     4501 gggacagaaa gagaaagaac agccccgccc cgtcgatatg gccggatgac ccgatttcaa
     4561 gaatcggaag gggatgccat gcgtagagga tcgagcggcc gggggacaac ccgaggaccg
     4621 tgaggagcct tagaatcagg aagcacgtga acaggtccat ggtcagcaat aggatcttaa
     4681 ggccccaaaa ggcctcgccg agataggcac ctgccgcaaa aatcagttgg gcggcgggag
     4741 gatagatggt gatgaaatcg gggtggttga tgagcccgag caggcttcgg gacgcctcgt
     4801 caccgggcgg catttcggag ggcgccgcgc ggtaggggtt gtgaccgttc gccagttgca
     4861 gtccgtccca gagatatcgg tacaggtcat cggagagttc gggaggacca aaaacgaaga
     4921 gaacccggag gccggcagcc acgaggagaa tcccggtcgg tgaccaccgg acggctcccc
     4981 ttccctctgc atacagaacc aaggcgagca aactcacaag gacggccgag accgccacaa
     5041 gggccgggac ggccgaagcc atgtcggact gaagcgccag cagggaatag agagcaagta
     5101 ggatggaaac cagcccccag gagagaatct gccggaacgc aatcttctcg aatggcccaa
     5161 cccttcgtgt ccgcgcggag accatccgcc tcaattcccc ccaacaggcc ttgattccca
     5221 gcagccggcc atgatcttcc catgatggga ccgcacttcc agcgccctcg tctcaactgc
     5281 cttcctgccg atgagagacc ctcttggcat tggaactaac aatttgatca attgaggttt
     5341 tccgtctaag ggaaagggac ggcctccgcg ttttcggaag acctgctgcc ccttggactc
     5401 tggttcttgg cccccgaccc ccaatccgtg caaagtggag ttgctcatgt gcgccctgaa
     5461 aacccgttcg ttctttcccg atggggattc tcgagaacat ctgcccgggg acccggcggg
     5521 aggtgacgac tcccgcggtg aaacggatct tgcccttttg ttgaaagccg tttcggcgaa
     5581 ttgcatcgag tgcgggcttt gccgcaaaga ctgtcagttc ctgaggaata ccggcaatcc
     5641 caagtccgtc gccgatgcgt ttgatccggc ggataaggcg gagcacagca agccgttcga
     5701 gtgcagcctc tgcgggcttt gcacctcgat ttgccctgcg gaagtggatc cggccgcact
     5761 gtttttggag atgcgaaagg aagcagtccg aagtgccgac tggaactgcc ccgagcactc
     5821 gacccttctc gactacgaaa ggcgagggac atcccgccgt tatacgtggt acgccctccc
     5881 cgagggctgc cgcaccgtgt tcttcccggg atgcaccctt cccgggaccc gaccggatcg
     5941 cgtgaaactg ctctttgatc acctaagaac caccataccc gacttgggaa tcgttctgga
     6001 ttgttgcgcc aagccctctc acgatctggg acgggaagcc ttttttcagt ccacattcgg
     6061 cgagatgagg gactatctgc tcgaaaacgg cgtccaaaac gtcctggtag cctgtccgaa
     6121 ctgccacaag gtcttcaaga accacggacg gacactcgtg accaaaaccg tatacgaggt
     6181 gctggcggac aacggccttc ccgaaagctc caagatctcc ggttccgtga ccgtccacga
     6241 cccttgtgcg gtacgattcg aggaacccgt tcacaaagcc gcacgagacc tggtcggacg
     6301 cacaggattg accgtcatcg aaatgcccca ccacggcagg gagaccttat gctgcggcga
     6361 gggaggcgcc gtcggcttct tgtccccgga gctggcaaag aactggcgcg ccatgcgcaa
     6421 gcaagaagcc caaggtcaca ggatcattac ctattgtgcc ggttgtgccg gttctctggg
     6481 gcaggtggct cccacaagcc atattctgga cctggtcttt gaacccgagg ccgtcatggc
     6541 gggccatgtg aaggtttccc gggccccggt gacctactgg aatcgcatcc gtctcaagaa
     6601 ctggttcaag aaacacctgg atgcggcggt gacccgagag aggaccttca cgacggagcg
     6661 gtcgagtgga aaagggggtc tcgccttgcg catcacggtt cttgccttgc tggtctccgc
     6721 catcgtggcc gtacgaacga cgggtgcgac acggtttctc gaacaggagg tcctgagagc
     6781 ttggattcag agctgcggcg ctttggcccc actgctgtac atgctcgtct actccgtagc
     6841 cccgtctctt ttccttcccg gacttcccat caccatcgtg ggcggggtcc ttttcggtcc
     6901 cttttggggt gtgatctaca ccatcaccgg ggccaccatc ggtgcctgcc tggccttcct
     6961 gatttcgcgc tacctggctc gggactggat tgccgccaag ctcaccagcc ccaggtggcg
     7021 aaggcttgac gaggaggtgc agcgccacgg ctggaaggtg gttgccctca cacggctgat
     7081 tccgctcttc cccttcaacc tcctcaacta cgccttcggg ttgaccaaga tcaaattcct
     7141 gcactacggg gtcacaacct ttgtgtgcat gctgcctgcc tgcatcgctt tcatcctatt
     7201 ttcgagttcc ctgctcgatc tgatccgtgg gaggatctcc ctcgaattcg tcatcgggtt
     7261 gctcttggtg gggttggttt ccctcatccc cgtcctgtac cggaggcggc ggggctgatc
     7321 tgaggatccg gaacggcccg gcgccgggct catcggccct tggcgcatcg gacccattct
     7381 tccatccccg ccacgtaatt gtgtgccggg ggcaacccgc agatggtgtg gaccgtccag
     7441 gcatagcccg agcggacccc gcctgcgcag tagtagacaa tgggcttgtc ggtatccacc
     7501 ccatggtcga caaagagctt gcgcaaggca gcggcatcca ggggacgacg atcttttccg
     7561 gaatagaaat cggtccatgg gatgtgaatg gcccccggca gatgtcccaa gacccaatcc
     7621 accgaggagc gggtgtcgat gagagccagg tcggcggccc tctcctgcac ttcggaagtc
     7681 cggatgtcca aatggggtct caggctcacc cgataccgaa tcccctgcga ttctcccctg
     7741 tcggtctccg tgctcaaata gtaccctcgg gccagccacg cctgtatgcc tccggccaca
     7801 agccggatgg gacccttgtg ccccagccag gagagtaccc agcaggccca tccttctcct
     7861 ccccagcttt cctctgcgtc tccatagacg gctacgggcg tcttctcatc gattcccatg
     7921 cgggccaacg catccgccag ctccccagca ggccgcaggc gaaaaggcac cccttgttcg
     7981 tccgtcgtcg tatgatcttc ccaggaaaac gggtaggctc ccgccacgtg gcccgcctcc
     8041 catctgcacc gaggcctggc gtccaggatc acccaggctt gccggttttc gtgcagttcg
     8101 ctgacatcga tgagagccag atcgggggcg gtggaagcgc cgaacgcatg ggttacgacc
     8161 cctccccaca acatgaaaag aaacaagctc ccgaccctga attttgcact tccccggatc
     8221 aactttgata ttgccgccgt catttcgatc tcctctccct tccgggcagt ccccgtgaca
     8281 tccccggtcc acgccgggat gtcgcgccga cccggccgtc atggggtctt tggacggggt
     8341 ttccgccctc cgacaacgca tgacttctcg cccacgaact cccgggctat gacccacaga
     8401 atcttcgtgc ctgctcggag cacaccgctt agacttccgc tgaccttgga ccgtcctgcc
     8461 ttccttctca gatagggaac aggatgctcg atgactcgca gtcccatccg gagtgccctc
     8521 acctgcatct cgatggtcca gccgtaatcc cggtcctcca ttccaagccc ttgaagagcc
     8581 cgccaggtga tcgccctcat gggaccgaga tccgcgtacc cgtgacccca aaccaacaag
     8641 atgagtcttg tggccaacca gttgccgaag cgttgctgta ggctcaatgc gtgagcctcg
     8701 acagggctcc gccgggccag ggccaaatcc gcctttcctc cggcgatcag ggccaccagt
     8761 tcgggcagat gggacagatc gtcactccca tccgcgtccg cgaacgccac aatctccggc
     8821 ggctcctgtt ccaggacctc aagagcggca agacaggcac gaccgtatcc cgctgtgggt
     8881 tcgttcacca cctgggcccc gtttgctcga gccaccctcg aagtctcgtc cgttgaacca
     8941 ttgtcgacca ccaagactcg atccacctcg tgggggaccg atcccaacac ccgcggcagc
     9001 gtcaacgctt cgttcctggc cgggatcacg agagcgatgg tttttccttc atacaaggat
     9061 gagaacctcc atggagagag tgaacgtcgg agcatcctcc gccgaaaaac gggcgaatcc
     9121 gctcgacagg ttccgataac accggaacag gatgctgtct aatcggtttt cccgccaaga
     9181 tcggcagcga tcaataggat gagccaattg aagatgccac agagccgaca aaagagagcc
     9241 gtttcggccc ggatggatca atgccggtac ggcgtcttcc tgccctgccg gcccttgggg
     9301 aatctcttgt acaggaatcg caacgcgact ttgaaggagt tcgtattgtc cgtgtccctg
     9361 cacctgaaga ccgtggttcg cacggcgagg cggagcaact ccggcaggtc ggcattggcc
     9421 ggctctctcc ttgcccggag aaacccgcaa aggaaacgct cagctccgag atcgagcaag
     9481 gtgcttacgg cgcgccgccc gcgggaagcc ttccgaggag gaaggggctt tcttttcgtt
     9541 ggaaagtgct cccagtcggg ttgaattccg cggcggggtc gggttaagct ccgcgccgac
     9601 cgatgtgcgc cccgggggat cggacggaaa ggtccggttc cacccggcgc gacccttgct
     9661 tttgtgccgg aaagattccg gaagctttca gcaacatgcg acgattgaat gaggattccc
     9721 ttgaagacac gaacggattt gcgcaacatc gccatcatcg ctcacgtgga ccacggcaag
     9781 acgaccctgg tggatgccat gctctggcag agcggcatct ttcgggacaa cgagccggtg
     9841 gccgaacgag tcatggacag cctcgatctc gaacgggaaa agggcatcac catcatggcc
     9901 aagaacacgg cggtggtgta ccggggagtg aagatcaaca tcgtggacac ccccggtcat
     9961 gccgacttcg gcggcgaagt cgaacgaacc ctgaacatgg tggacggagt catgctcctg
    10021 gtggacgcca ccgaaggccc ccttccccag acccggttcg tcctgggcaa ggccctggcc
    10081 cggggacttc cgcccatcgt ggtgatcaac aagatcgatc ggcccgaccg gcgcatcggc
    10141 gaggttctcg acgaggtctt cgatcttttc atcgacctcg acgccaccga ggagcagctg
    10201 gactttcccg tcctctacac caacgccaag gccggagtgg ccctcacgga accggacggc
    10261 cggggacggg acctggtgcc cctcttcgaa gccatcctgc gctccatccc ctccccttcc
    10321 tgcgaccccg gggccccgct ccaattcctg gtcaccaccc tccaacacag cgactacgtg
    10381 ggccggattg ccgtgggaaa ggtcgtgaac ggcactttgc actgcggaca ggaagtcacc
    10441 ctcctcaagg cggggattcc tctccgaaag acccacctca gcgcgctgta cgcctacgag
    10501 gggctcaacc gggtggaacg gcggacggtg gaagccgggg atatcgccgc cgtggccggg
    10561 gtcgaggacg tcttcatcgg ggataccctg ggagaccccc aagatcctcg accgctgccc
    10621 gtcatcaccg tcgaggaccc gaccctctcc atggtctttt ccgtcaacac ctctcccctg
    10681 gccgggaagg aaggaagatt cgtcacttca cgacatatca aggaacggtt ggacaaggaa
    10741 ctcctctaca acgtgagcct gcgggtggaa ccggcggaga accgggattc ctatcgggtg
    10801 agcgcccggg gggaactcca actggcggtg ctcgtggaat ccatgcggcg ggaaggtttt
    10861 gaactctccc tgtccaagcc caaaatcatc actcgaaccg tccacggaca gctggtcgaa
    10921 cccaaagagc tggcggtggt ggatgtaccc gaaaagttcc tggggatcgt caccgagatg
    10981 ctcagctcac ggaagggccg gatgaccaag atggtgaaca acggcttcgg gcgcgtccgc
    11041 ctcgagttcg aactcccctc ccggggcctc atcggctttc gaagccagtt cttgacggac
    11101 acccggggca cgggaatcct caacaccctg gtcatcggcc atactcccta tgcgggagag
    11161 atcgtcggcc gacccaacgg ggtgctggtc tcggaccggg acggaaaggc ggtgacctac
    11221 gccatccacc accttcaacc ccggggcacc atcttcgtca atccgggaga tcccgtctac
    11281 gcgggcatga tcgtgggcga gaactcccgc tccgacgaca tccgggtgaa catcaccaag
    11341 gagaaaaagc tcaccaacat ccgggcctcg ggatccgacg aagccctgcg cctcatcccg
    11401 ccccgccaat tcaccctcga acaggccatg gagtatatcg gcgaagacga actggtggag
    11461 gtaacccccc aatccatccg cctgcgcaag atgaagctga ggtaggccgg ggtgctggga
    11521 ggccgggaag gaatgggcga ggggccgggt tttgatcgca ccgggttcgg atcgtgcggg
    11581 aagccgggat gccgggatgt cgtgaggggc gtagggtggg tggagcgaag cgaggcccac
    11641 cctacaccgc ttcccaaccc ccagcctcca gccttacgcc cctagccaac cgaccgacca
    11701 accaacccct gccccctcgc ccatcccctc cccgctttcc agtctatggg ccggacagaa
    11761 gcacctggac cgcactgtcc gcaagttcca tgaggtaggt ggggaagaac ccggccacga
    11821 ccatcccgga ggccaacgcc ccagccagga tgcgggtgga taagctcacg gcccggggac
    11881 ccgtttcttc tcggggttcc gtaagatagg ccgccttgac gaccagcaga tagtagtaca
    11941 aggaaacgac cacgttgatc atggcgatga gaaccagggc gaagtgccct cgttccatgg
    12001 cggccacgaa caccaggaac ttggccgtaa agccgatggt cggggggatt cccgccaggc
    12061 cgaaaaggga aaccatgagg gtgagggcca ggatgggcga tctgcggtgc agccccgcca
    12121 ggtcttccac ctcgaggttc tttccgtccc cggccaccac cgtcaccacg agaaacgccg
    12181 aaaacttcat cagcagcaga gccatggcgt agaagacggc cccggcgtac ccggactcgt
    12241 tcacggccag gattccgatg agcacgtaac ccgcgtgggc cgtggtggag aaggccagga
    12301 gcctcttgag gtccttctgc gcgatggcgg ccaggttgcc cacggtcatg gacataatgg
    12361 acaggaccgc caggatctgc accaggtgcg cgcttccctg ccccgccagg gccacgaccc
    12421 ttaccatcac ggcgacggcg gcgaccttcg aggccgtggc gatgtaggcg gccacctggt
    12481 tcatggcccc ctggtaggtg tccggggccc agaaatgaaa ggggaacacc gccagcttga
    12541 aaaacagtcc tccgagagtc atcagcaggc ccaggaacgc cagggggcgc tccgccatcc
    12601 ccgacggggc gcgggccata tccgacaggt aagttgccgg agccgaggcg tacagcaggg
    12661 ccaggccgaa gagcatcacc gccgacgcaa aggttcccac gagaaaatac ttgatccccg
    12721 cttcggtccc gaatcgttgg tcccgtcgaa cggccaccag gatgtacagg gagtagctcg
    12781 agagctccag ggacaggaat agggccaaca agtgatcggc gctcaccagg agcatcatgg
    12841 ccagggtgca cacgaacagc agcacgtaaa aatcgtggcg atggcgctcg cgaaggtccc
    12901 tcatcttccc gcacagggtc aacaccagaa acaggctcaa cgacagcagt tccttgaaga
    12961 cctgggaaaa gaggtccacg cggaaggccc gggtgaaaag gtacccttcc tgcccgatgg
    13021 agaccgtaca gatcgccacc cccagtcctc ccagcagaag cgaggtgcga tgttccctcc
    13081 gggcgtcggg tttggccgcc agggacagga ccagaaacca gagggccgag gcaaaataag
    13141 aaatctcggg agcggcgatc agccagttca tctcgacagc ccaaccatga agggaaccga
    13201 cgccgtttga atcacgtcaa agaggagcgc cgggtaaagt ccaaatagga ggagcaccgc
    13261 cagcaggatc atccggggaa ttccctgcac cagggaaatg tccggcaagt gtgcatattc
    13321 ctccttcggg ggaccgtaaa acgtgtgctg gaccactcgg agcatgaaca gagcgctcac
    13381 caccagggca aggacggccg tggttcctcg caccgggtac accttgaacg cggcaatgaa
    13441 gaccaccagc tctccccaga agcaggccaa ccccggaacc cccatgcccg ccatggccgc
    13501 cacgatgaag taggcggacg ccacgggcat gacccggctg agccctccca gttcgggaat
    13561 catcttggtg tgggtcctgt cgtagatgta gccgacggaa gagaacagca gagccgtcat
    13621 gactccgtgg gcgaacatct ggaagacggc cccgtccagg ccttccagcg taaccgtgga
    13681 taggcccagg cccaccacgc ccatgtggga aacgctcgaa tacccgatga cgtacttgag
    13741 gtccgtctgc ctcaacccca ccaaggcccc gtagagaatg ccgcaggcgg cgagcaacgc
    13801 catgagatcc gcccattgct tccagccctc gggacacaac atcacggcca cccggaggac
    13861 cccgaaggcc ccgattttca tcaagacgcc cgcgtgcacc atgctcacgg cgtgaggcgc
    13921 cgccacatgc cccacagggg accaggtgtg gaagggccac acgccggcca gaatcccgaa
    13981 ccccaggtac agcaggagga aggcgaattt ttggtcctgc ggggtgaaga cgccggactg
    14041 catcagggaa accacgtcga aggtgttcag cccggaccgg acgaaaagaa agatcaccgt
    14101 cggcaggatg agggcgctgc ccgccagcag gtacagggtc agcttcatgg ccccgtattc
    14161 cttgcgggtg cttccccaca cggcaatgag caggtacatg gggaacaggg agacgtcgta
    14221 ccagacaaag atgaagaaca ggtccatgct catgaacagg ccgaagacgc cggtcaccag
    14281 gacgaaatag agggcgtaaa attccttcac ccgggattcc atcccccaca tggtgaggac
    14341 gccggtgaaa aagacgatcc ccgtgaggag caggttggga aggctgaagc cgtccacccc
    14401 gttgaagtaa tgaatcccca gggaaggaat ccaggcgatc ttttccacga actggagacc
    14461 gcccaggtgc cgatcgtagg acacaaacag gcttaccgac aggaataggg ttgccccgga
    14521 aaagacggcc ccggctttgc gaatgagatc cttccgctcg tcggggacgg ccaggatgac
    14581 cccgagtccc gccacgcagc tgaacaggat ggcggaaaga taaggaaagt cggcgtacgt
    14641 caagtgcatg ggtccaacgt cctcggtgtt ctcacaggaa gaaatagaga gccaggaaca
    14701 cgacgaccgc aaaggttgtc aacagtcggt actggatcat cccggtatgg aggaaagcgg
    14761 tgattcggcc tcccgccgcc gtgctctcgg ccgttccgtc cacgaaaccg tcgatgacct
    14821 tgtgatcgtt gagataaagg aggcgggaga tgccgcggtc gatcccccga tccacgagga
    14881 acccgtagac gtggtccagg taccaacgct gggaaaacag gcggaagagc ccaggcagtc
    14941 gttcgacaaa cccgacccgg ggagcgcccc ggcgcccgaa ttccacccag gacagcccta
    15001 ggcccagggc cgcaagagcc acggacacgt agggcagcac gggatggtgc aacggatcgg
    15061 gccaatgcaa gccggagatc tcgccgcccc gaaggaattg ctcgaggggc acctggaaaa
    15121 gacccagcaa gagggtaacc ccggccagaa cgatcagcgg cccgtccagg atccactcat
    15181 ggaatcgcga gtgggcggac ccgtgggagg gcgccgaagc ctcgggcgcc tctccgtgtc
    15241 cgggacccga ctcccccggg aaaagcagga taaaaaccag ccggaaagca tagtacgagg
    15301 tgaggaagac ccccaacaga cccgcaccca gccagagcgg gttcgagagc tcggccaggc
    15361 ttcccaggat cacttccttg ctgaagaatc ccgaaaggag cggcagcccc gacagcgccc
    15421 cggcggcaat gatcaagcac gccatgggaa tcttgagact gcggcagccc tgcctgccca
    15481 gctcgaagat gtcgttggtc gcgtaccggt ggataaaaac tcccgaacaa aggaacagca
    15541 aagctttgaa ggcggcgtgg gtggtcaggt gaaaagcccc cgaaaaaagg cttccggcgt
    15601 ccagccccat gagcatgaaa cccagctggc tgatggtcga gtacgcccag atttgcttga
    15661 tgtcccggtt taccatggcc atggtggagg ccaggagcat gctgacggtg ccgacggcaa
    15721 gaccgacggt catggcggtc cgggaaaaga gaaagaaggg atagagcctc tcgtacaggt
    15781 agatcccggc cgccaccatg gtcgaggaat gcagcagggc gcttaccggg gtgggcccct
    15841 gcatggcgtc gggcaaccag gtcaacagag gaaactgggc actctttccg acgatccccc
    15901 caaaaatgag cagggccgaa agggtcagca gttcggggga catggaagcg gccgccttgg
    15961 ggctgtccat ctcgaggatg tcaagatttc ccaggtggag caacacgagg aggattccca
    16021 gaaacatggc cgcatccccg agccgggtca tgacgaacgc ctttttcccc gcctggctcg
    16081 ccatgagttt ttcgtaccag aagccgatga gcaggtagga cgacagtccc accagttccc
    16141 agaagatgta gagctgaaac atggtgggag aaaccaccag gttgaacatg gcccatacga
    16201 acagggacat gaaggcgaag taccgggaaa aaccgggatc cccggccata tatcccaggg
    16261 aatagacctg gatgagaaag ctgaccgcgg cgaccacggc cagcatgagc aggcttacgg
    16321 gatccaggag gtagcccgtg cagcataccg cccgatggtt ggtatgcagc ataaagcccg
    16381 tgcagcatac caaccatcgg gcggtatgct gcacgggctt ttccatattc cagtgaaggg
    16441 tcaggagaac cagggtcccg gctaaggaaa ccgacgaaac gccgacggcg acggccgcgg
    16501 agagccgggg gcgggatcgc gtaaagagca gaatgaccac gaaggccgca aaaggcagca
    16561 gggttgccaa tgcgacggtg ggcagggtca aggtttcagc ggaaaatcct gtcatgtccg
    16621 tcttcccgca agggctccta ggactcggtt acgagctact tcaaggagtt gtaagcctcc
    16681 ggatcgaagg aaccggtcct tcgaaaggcg tgcaggagaa tggcaagccc cagggatacc
    16741 tcggcggcgg ccaccgccag gatgaacaac acgaaggctt gtccctcgag ttgctgccag
    16801 tgcagagccg aacccacgaa agcgatggat gcgccgttta gcatgatctc gatgcccaag
    16861 acgatcatga tgaggtttcg gcgggccagg gcgcagaaca tccccaggga gaaaaggact
    16921 ccggcaagca ggagaatatg gctgaaaggg acgatcatcc ttcctcctcc cgggacaaac
    16981 cttcctgctt cctgcccagc tgaagcgccc cgatgagggc gaccaggagc agcaaagaga
    17041 tgacctcgat gaaaaaccag tggttctgca acacatagag tccgaagtcc cggggagcgg
    17101 cggtgagcat tttgagagga atttcggtgc ccggggcggc gagcatcagg acggagcccg
    17161 ccgcaaggta caccagactg aagagcgcgg cgggaatcca ttgggacagc ggaaagaaat
    17221 gttcccccga agcctcgacc cggatcatca tcaccacgaa caggaacagc accatgacgg
    17281 cccctgcgta gatgatgacc tcgagggccg ccaggagggg cgctcccagc aggtaaaaca
    17341 gcatggccgt gccgaaatag gagaagatca ggtacaccac cgcatgcacc aggttgcggc
    17401 gggtgaccgc cacggcggtg gatgccgtca cgaccgccgc cagcacatag aaaagaaatc
    17461 cgtacagggt catgaaaccc tcgcaaaaag ggaaggcgtg gaccggtttc tacggcaagt
    17521 tgctcttgac gttcaccggt tccttctccc tgaaatgttc ccccttgccg cccgcgtccg
    17581 tcgcgatccc ggcaacccgg tagaagttat actcggggtc cttgccgcaa tgattcacca
    17641 ggagatcctc cttctcgtag acgaaatcca ggacctccgt tttgcagaat tcaaagtccg
    17701 gggtgagctg gatggccgaa gtgggacagg cttcctcaca gaggccgcag tagatgcacc
    17761 gggcgaaatt gattcgaaac cacggggcgt agcggcgccc gtcgggcttt tccgccgatt
    17821 ccatggaaat gcagctcacc ggacaaaccg cggaacacag gtagcaggcc acgcagcgct
    17881 cgcggccgtc ggggtcccgg gtgaggacga tgcgggctcg agtgcgcgca aagaggggcc
    17941 ttttgtagtc cgggtattct tcggtgatgg gtttccgaaa gagatgcaca aaggtttcgg
    18001 cgaatcccga agccagcgct ttgaccgcgt cccaagccga ttccatgggc gtccccgttt
    18061 cctttcttcc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggagacggg
    18181 gatgagcacc ttccagccca gggccatgag ctgatcgtac cggagtcggg gcagggtccc
    18241 ccgcacccag atcatgaaaa tcgccacccc gaggattttg accaggagcc acacgaaggg
    18301 cggcaaaacg gggcctcgcc atccccccag gaagaggacc gcgacgatgc cccccagcac
    18361 catcatgttg acgtattccc ccaggaaaaa gagcccgaac cgcatcccgc tgtattccgt
    18421 gtggtacccc gcgatgagct cgttttccgc ctcgggaaga tcgaaaggaa ttctcttgct
    18481 ctcggccatg gcgctcagca ggaaaaccac gaaggccacg ggctgcacca ggacgaacgg
    18541 atacgattcc tgggcccgaa cgatgtccac catgctgaag gaacccgcca tcatgacgat
    18601 gggaaccagg gaaagaccca gggagagctc atagctgatc atctgggccg ctccgcgaat
    18661 gccccccagc aggctgtact tcgagttgga agcccagccg cccagggcga ccccgtacac
    18721 tcccagggag gacagggcga aaacgaagag caggccgata ttgaggtccg tgaccaccag
    18781 ggggacggac cgtccccaga aggtgagatc ttttcccagg gggaccacgg caaacatcag
    18841 gaaagccgtg gtggccacca cggccggggc caggagaaag aacggacgat ccgccccttg
    18901 gggtaccgtg tcttccttgg tcagcatctt caccaggtcg gcaacgggct ggagcagccc
    18961 gtacttgccc gcccggttgg gaccgtagcg gatctggagt cgcgcaagga actttcgctc
    19021 gagccagacc aggtaggccg ccatgaggag caaaagcacc agcaccgccc ccagtttcac
    19081 gatcaggatg aaaaatcccg ccatccaggc cagcatagcc cgtcactccg ctcccaagtc
    19141 acgaaacccc tgaatccctc cgcccccagg cccctcgcca accacccgac taaccgacca
    19201 actgattgac caaccaaccc ctcgcccctt gtgaacctct ccccggtggg cttcgctttg
    19261 cttcacccac cctacctcct cgcctccccg cttcccagcc gcccgctcgg tgggcttcgc
    19321 ttcccccacc ctacaccgct tcctcgcccc tcgcccccta ccggtcgata tccggcagga
    19381 tgaaatccag ggacccgatg atggcgatga ggtccgcaat gctttcgccc tccgccagga
    19441 tgggcatggc ctggacgttg gcaaaacccg gtgcccggat tcgcagtcga tagggatggc
    19501 ccagcccgtc gctcaccacg tagtagccct gctctccccg gggaatttca caggacacgt
    19561 aggcttcccc ccgggggatc ttggggcctc gggtaacgtt cacaaaatgg tgaatgaggc
    19621 tctcgatgtc ccggagcatc tcgtcccgtt tggggaccac gtaccgatag tcgtcggtga
    19681 tggtgcggcc cggcggcatg ttcctgctcg cctgttcgat gatccccagg ctctggcgca
    19741 tctcctcgac ccgcaccagg taacgggcat agcagtcgcc ccccgcggcc gtggggacct
    19801 cgaattgaaa ccgctcgtag ccggaatagg ggaatttctt tctcaggtcc cattccactc
    19861 cgcaagcccg aagattgggc cctgtgactc cccagtccac ggcttcttcg gcggagattc
    19921 ggccgatccc ccgagttcgg gccttgaaga tggggttctt gctgatcagg cgctcatatt
    19981 cttccaacct ccgaggaaag atgcggacga agtcgtccac ggcctccttc cagccttcgg
    20041 gcaggtcccc ggcgactccc ccgatgcgaa accacgacgg gtgcaacctt cccccggtga
    20101 tgcgctccac gatatccagg atcatttccc gttcggcgaa ggcgtagaaa ttgggagtca
    20161 tggcccccac gtcgtgggca taggtggcga accacaccag gtggttgctc aggcggaaaa
    20221 gctcgctgag aagcacccgg atgacctggg cccgctccgg cacgcggatg tccgccaggg
    20281 tctcgacagc cagcacgtag ggcaggtcgt tggccgcccc ggcgaggtag tccacccggt
    20341 cgcagtaggg gatgaactgg tgccacgtct ggcgttccgc aatcttctcg ggaccccggt
    20401 gatgataccc gatgtccagg tccaggccgg tgatctcctc tccctccagg gatacgatgc
    20461 agcgcaagag cccatgagtg ctcacatggt ggggcccgat gttgaggaga aactcgtggg
    20521 tcccgtcttc aaactttccg tccagcctca tgaggacttc cgcatccagg ggctgctctc
    20581 ggcgagcgtc ttcacgggtg tagggaggca tttgagtggc ccgtcccggg tagctcttcc
    20641 tcagggggtg cccttcccag ttgtgaggca gcagaattcg ccgcaggttc gggtgcccct
    20701 cgaaccggat gccgaacagg tcgaagactt cccgttcgta ccagttggcc gaaggccaga
    20761 tgccggtcac ggacggggcg gcgggggaat ccccgttgag acccaccttg aggcgcaatc
    20821 gaacgggagt gtccaaggaa agcaggtggt acgtcagggt gaaatcggga tagggtttgg
    20881 gcaagcgttg agcgtaatgc ccggcggcac ccacgtcggc aatggcgctc gcatacccgg
    20941 ggtcccaggt tcgatcccgc cgggccgatt cgtccacggc ggtcaggtcg tccaaacgct
    21001 ggaaccgggg gctggattcc ttctttagaa atgcaagcac ctcccggatc cgcgattcgt
    21061 ccacctcgaa ggcggccata tcggacgtgg ggaattcccc gcgcacggca tcgccgaagc
    21121 gttccgtcag ttgagcccca agggagcggt ctcggctcga aagctcgatg cgccgggcag
    21181 ggggagtcca catcccgtcc gagcgactgc cccaaaattc gggcggggtg acggaagttc
    21241 cccttatggg aagcccttcg tatccgggtc ccgggggcgt tcgggacttg gtccggccat
    21301 cctcgaggac gggctttacg gtgccctggg ttccgccggg aaggtgaaaa acgcatcggc
    21361 cgggcttctc ggtggagacg atcttgtcct gtagcaggac gagcccctgc agaacggctt
    21421 cgggtcgcgg gggacacccg gggatgtaaa cgtccacggg aagaatctgg tccaccccct
    21481 ggaccacgct gtacacgtcg tacattcccc cgcaattgga acaggacccc atggaaatga
    21541 cccacttggg ctcgggcatc tgttcataga ggcgaaggac caccggggcg atcttcttga
    21601 agaccgtccc cgagacgatg agcagatccg cctgccgcgg cgaaggacga atcacctccg
    21661 ccccgaaccg cgcgatgtcg tagcgggccg taaacgccgt cgcctcctcc acaaagcagc
    21721 acgagagacc gaaaaagagc ggccaaaggc tgttctttcg agaccagttg atcagcagat
    21781 ccgctttgct ccaccaccga tcgcttattt tgccttgtaa cccggtctcc aatcgagtcc
    21841 cccgtgtttc cagacataaa gaagcccaac gaggagcaac acaatgaaaa acgccatttg
    21901 aatccacccg ggccagcccg cttcccggta gatcacggcc caggtgaaaa tgtaggctcc
    21961 ctcgacgtcg aaaatcagga agaagacggc caccaggtag aagggaacgg ggtaacgaaa
    22021 gcgggccgtc cccgttggaa cgatgccgca ttcatagggt cggagttttt ccgcgctgat
    22081 ctttttctct cccagccatg ccgccaggaa gagctgggaa gccacaaagg caaacaccag
    22141 cacggcgtag accgtcagag tgaagacgcc gggcccccaa ggcgtcggga tcgcctcccc
    22201 cgcaatgggt tgcatgcacc ctccctgttc cctgaatgat cgtaaggaaa aacccgttcc
    22261 gaaaaatgcc tgttcgaccc tccccggcaa caccccccca gaagccgtta ccggccaatc
    22321 taagacacat ccttccctta gtaaacgacg gatttgattt tgtgggggac acgggaagat
    22381 gagaaaggaa aagcggcggg aaagaactcg attcccttcc ccgccgcacc ccggggtctt
    22441 gcgaacggta cgccccggct accgggcctt gctgaaagaa tggcactcgc tgcagtcata
    22501 ccctttccga ctatggcgcg ggtgcacgga accgaaggaa tacttcttgc tctcgtggca
    22561 ggtcttgcat agatccgcca ccggcttcac agcctcgtat cccatgctca acagcggcat
    22621 ccggggagtc gaggttccat gacaggtggt gcaggatcca gcgtacccga gagcgtcttc
    22681 cttgggggac acttcgtgga acagtcccat ccatcgctcg gtctgccgga aatcgtaggg
    22741 gttgcggggc cacccgacgg cgtcctgacc gtagggaacg gcgttcacca ggtccccttc
    22801 catgtaaaag agcccgattt tcaacgggag caggtagcgg gtttcgagat ccatgggctg
    22861 gagagccgtg tgaactttga acggataaat cttggagccc cctgcgctca cggatcccac
    22921 cggtcccgcc atgaggacgt tcccgttggg ctgcgcatcg gcgggggtgc caaaatcata
    22981 gaaagtggag gtctgtccgt cccagaatcg gtactggggc gtgacattgg ctttcatggt
    23041 tcttgcggga tcatagaggc ccgtggcaag gttaagctct cccggggccc cccaatcccg
    23101 aaacacgtcc gtgggcgcga ctttggcaaa ggacgggatg tggcacgtgg tgcagtggac
    23161 acgttggagg tgataggcat tgatcttcga gtcgccgtga ggaggcgtgg gcgtgtggca
    23221 cgtggtacag tccggccggg tgccttcctg gactcgaagg tcggcgccct ttcccgcaat
    23281 cttgtgaccg gaaacccggt ggcactgagt gcagtgcagg tccccacctc ccaaggacgc
    23341 cggggacata tgaacatcaa aagacctcgt ccccggagat cgatggtgtt cttccaggtc
    23401 tccccgtttg aagttgtccc ctcccccgga cttggtgtga caatgaagac aggtgtcgcg
    23461 gcttgtcttt cgaatgtcca ccgcagcttc caagatcgtc attcccatct tctcttcgtc
    23521 gggaatgaac cttgccttgg tgaccgggtc caccgtgcgc ttgtactggg gcgcatggca
    23581 gatcaggcag tcgacgtttt ccagttgagc atccgtgggg gaaaccgccg ccacgggctt
    23641 ggctcccatc cccacgtggc acttcccgca tccggcatcc acttccaccc cgtaggcgtt
    23701 ggtcaggcga ccgatgtggt tgggatccgg atagatgcaa aagtcgttga tggcccccaa
    23761 cttccccctc cattggtaat gttccgaccg gtagacctcg acggcacgat ctcgatggca
    23821 ctgcaggcag gttttcgttc cttcaaagac ggcgaatttg tccgcatggg atccggagag
    23881 gggcggcagg gaatcgaggt ccacgatggt gaccacgagg actccgctcc gggtgattcc
    23941 gccggaagaa tacgtggccg tgagggtgac ctcctcattg ccgcccacct cgagggcggt
    24001 caagactccg ggagatccga accgggcatt ggcggacccc tcgtcccagg aggcttcccc
    24061 ggtcacgttc cgctgggtcc cgtcgctaaa ggaagccgtg gccgtgtagg aggagactgt
    24121 cccctcgttc accgaggcgg ccccggaaac ccggatatcg gtgagggccg ccttcgcaac
    24181 ggccgagtta acgatcgtta cccttttctt agcggttttg atgattccgc ccgacgaata
    24241 cttggcggtg atggtgagct gtttttccac ccccaccggc cgggtcttga gcacccctgc
    24301 ggatatgacg gcaaaggacg agttctccga ccaggccgcc ctgcccgtga ctcgtttccg
    24361 ggtaccgtcg ctgaagacgg cgacggcctt gaacctggcg gaaccgcttt ccggcacggt
    24421 gtccggcccg acaacggaca atcccgtcaa gaacacgcct ttgtcccccg cgcgggcggc
    24481 cgaaactccc caaaccaccg caaaggcgag tacgacggcc cagagtaccg cccggatgaa
    24541 taattccaat tccccggatc tctcaccatg gctttcacgc atcccatacc tccaaatgaa
    24601 gacctgaagc cgattactgc cgaatcgccg ggtcggacct cgatgaatgc ccgcctgaac
    24661 gtgggtcgaa atcccctacc ccggcagtgg gaaatcccca tcgggcggat tgtaagcaaa
    24721 actaatgcct cattccttgg aaatacagac aattggaaga tatccacaaa atattcgtgt
    24781 tccaggcaag ctctgccttg tttttcaacc atccgttttc gcaagcctcc ttcccaaacc
    24841 ctcgagttcc atggggccca tcccttaaca cccgttgctc tcggttatac tcgtttaggt
    24901 ttgtgggtga cgttccggcg cctctcttct ttgctttggg aaaggcatct tgccgctacc
    24961 acgcaatttc gcggctggat gccgctccta caaaaacgaa atttcaatct tcgaggggca
    25021 tataaccttc cggagccccg gaaagggatt gatgcaggcg tcttgtcgct gatagcataa
    25081 ggatcggtgc ggcgcctttc cgtcggggag cagttcggga gacccgtccc gggccatggg
    25141 ctgcgagcca gatccccggg gcggcctccc gccggttttt cttcgagctc ggacgggcag
    25201 ccgacttgcc gatgctgttg gatgggttta tgggcctggt attgctggga atcatttccg
    25261 tgattgttgg ggtcctgatt ggaaccgtgg gcatcggagg gattctgctc atcccctgcc
    25321 tggcctacat cgccgacttg tccacccatg aatccatggc caccgccctg ttcagcttta
    25381 ctttcaccgg cctggcgggg acctggatgt tccagcttcg ccgcaccatc gattggggga
    25441 tcagcctgcc cgtatgcatg ggcgcggtct tattcagcta cctgggcgcc cgagtgaacg
    25501 ccggggtgga cccccgcatc ctcaacgtcc tcttgagcct gatcatcctg ttttccgggg
    25561 tctacaccct ccgttcctcc aagggcgacg gcttcgaata cctggacggc cgatctgcgg
    25621 cacagcgcct gcttttactg gggaccggag ccgtcgtcgg attcgggtcc ggtctgaccg
    25681 gcaccggtgg gcccgtcctg tcggtaccgg tcatggtgct catggggttt gcacccctca
    25741 catccatcgc caccagccag gtgctccaga ttgccgtggc atccgcgggg accttgggta
    25801 accttcagaa cggttcggta cacctgggtt tggggctttc cgtcaccgtg ctggaactgg
    25861 ccggggtcgt cctgggagtg cgcctggctc acagcctggc aaccgtccgg ctccgccaat
    25921 gtgtgggggc ggtctgcgtc ggggtcggtc tgctgatcct cttccgatcg agggagattc
    25981 ttttcatcgg ttgggaacat gtgctccgtc ggatttgagg tgaggattcg tccggaggcg
    26041 agggaaaaag ggcccgttcc atgagggttg ggtcggcggg aacgaggatt cgtccggagg
    26101 cgagggggaa aagattgaaa ggaagggaag tctcatgcct atgaatcccg cagatcggga
    26161 cctggtccgg gagtggggaa aaaaactttc gggtccgtgg ttgcttaagg ccgttttgac
    26221 cgacgacccc cgctccccaa aactcgagtc tttctatcgc gccttggcgg agacggtttc
    26281 atccccggat ttcatcgtgg aacggagacc ggggcgggaa ttccccgaac tggtggtggg
    26341 ggaagtctgg cggtttcaca tggttccgtc cggcagggaa ctcgagccct ttcttgaggt
    26401 gatctcggcc atggcggatc cccgacccct cccgctcccc acccattccc gggaactgga
    26461 aatgccgctt tggagctcgg acctccttct gttcgtcacg cctcagtgtc ccttttgccc
    26521 cgccgccgtc cgtcaggtca tccccttaac ggcctccccg gcccgatctc gggcaagggt
    26581 ggtggacggg gcactctttc cggagatcgc cgagcagcac ggggtgaagt cggtccccac
    26641 cctgatcctg aacggcaaat ttcgcatgac ggggacgatc acgctggatg aaatcctccg
    26701 gattttgcac cggtccgacc catccctctt ggacaccgat accctgctgc ggatgctcaa
    26761 ggagggtcag gcaggcctgc tggccaatat gatggtcgag aagaataccg tcttcgaggg
    26821 atttcttccc ctcctcgtcc acccggaatg gaatgttcgg ctgggagcca tggtggtggt
    26881 ggagaccctg gccgaaagga acccccccct tgcgggggag ctgctcgata ccgtttggga
    26941 ctcggtcccg ggcgcggact cgcgggtcca gggagatttc ctgcacctga tcggcgaact
    27001 gggggattca aggtggctgt cccgcatgga agaccttgcc cggaggaacc cggacccgga
    27061 agttcgcgag gttctcgagg aaaccctcga actgctcagg gagcggccgt agggttccat
    27121 cggaggggtg agttgcagac ggaacgggac cgggggctca tttgaagatt ttgtgccagc
    27181 gggacaggcg cttcttcatg gcttcctccc cttcccccct cgtccgatag acaaaatgca
    27241 ggtaggcaca gatggcgggc agggagaggt tcttgggttc ccggtagaaa tcgtccatcc
    27301 cgtccaccac gtcccccacc gtatacttgg acggaacgcc ggccgcactg aagatgggat
    27361 ccaccacgct cttctttacc ccttcggcat cggccatggc ccaaaacagg gtgattccgt
    27421 cgaagagccc gcagacatac cccaattttc cgggatacgt gagtttgtcc caggccttgc
    27481 cgttgagcca gttgcgttcc acgaacttca ccaggaactt gtcctcggaa gagacggctc
    27541 cccaggaagc cgggagagcg gtccccagga ggaagaaaaa caccgtgcat gagaggatgg
    27601 tccctcgact tccttgaggc ttcgtcatga tatgctccca gagtgtgtat tgcgagttcc
    27661 ctccggcaga ggggtcccgg cgcaccccaa ccttgatgtt ctcgattgca gggataaggc
    27721 atactcatag cacgcgtttc cttggagatc aaagaaggac accccggcac aggcaaagaa
    27781 ttccggggac cccattgggt cgttgcgtgc ggaacgcaat tgtgcttata tgtcgccatt
    27841 cagtctcggc ggaatccaat caacacctac ccgaaacctt actataacga ggggagacat
    27901 atgaaacgga cctttatcct gaccttggta gcgatcattg cggtggtctt tgggacatgg
    27961 catccggcgg cagcccaggc aacaccggaa caggctcccc aagtccaacc ccggatggga
    28021 tgtgagaaaa acttcgaccg attggatgcc aaccaggacg ggcaggtaac caaagaggaa
    28081 ttcatggcgg ctccccatcg gacccgcaac ccggaacgga tgttcaacaa gcgtgaccag
    28141 aacggggacg gaacgttgac caaggaggag ttctgcgcca caagaggccc cggcagggga
    28201 atgcgggggg gaagaggctg cgaaaagaac tttgccaaga tggacgccaa tcacgatggg
    28261 caggtaacca aagaggaatt catggccatt tcccatcgaa gcgccaaccc cgaacgcatg
    28321 ttccagaaac gggacgccaa cggggacggg gctctgaccc tggacgaaat gtgctccgga
    28381 cctccaccgg gccagggaag gggtagggcg ggaacccagt aggttccggt ggggtttgcc
    28441 tcgattcgat ccgcgcctct gcttcccggc gcagcgatgg gagaatatcc cccccgttgc
    28501 gccgggagcg ggtcctcctt ccacccgatg gagcgggcag cttcttcccg gatcaacagg
    28561 atgggcgccc atgtctttgg gtgaaaatcg gtttcggccc cggtcgccgg atttgttttt
    28621 ccaacgacgg cgaagagctg tttgccctgc tcggagaaga ggatcgaaga gacgacaggc
    28681 ggcggatacg gaagtcgcca tggcctgagg gagggactag aagggcggaa gcaaccacag
    28741 ccttgccgtt cgcctccgac tgatctgagg atgcacctgc catcctccaa acaggggatt
    28801 tccaggtccg cgtttcctgc tcccagtggg gaaatgcgcg acctggcgcg atttgaccta
    28861 tggagggaga cgcttatggg ccatgttgaa acctcggaaa aagaaggaat tgcaaccgtt
    28921 agcatttcca ggggaaaagt gaatgccatc aatgaggtcg tggtcgagca aatccaggag
    28981 gccctttcgc gaatccaggc gaacgaaacg atcagagccg tgatactgac cgggaggggc
    29041 aggttcttct cctttggttt cgacatacct gaattcatga gtttttccag ggaagatttt
    29101 tcgcgctatc ttatcaaatt caccgatctt tacgcccgca tcttcctgtt tcccaaaccg
    29161 gtcattgccg ccctgaacgg acataccatc gccgggggtt gtatgattgc ctcggcctgt
    29221 gattaccggc tgatggtgaa gggaaaggcc aagatctcgc ttaatgagat aaccttcggt
    29281 tcctcggtct ttgccggcag tacggccatt ctgaaatact gtgtagggac cgtgaacgct
    29341 caagaaattc tctactccgg aagaatgtac accgccgagg aagccctggc cctcggactg
    29401 gtcaacacgg tcacatcgga agacgacctg ttgatcaaag cggaaatggc ggccaaggaa
    29461 tttgccgcca aagatcccgt tgccttcaag agcatcaaac gaatcctaag aagacgcgcc
    29521 atggaagaga ttgctcgatt cgaagaggaa tccatcaagg aattcgttga aatctggtat
    29581 tccgaaacca tgcgccgaaa tctgagtcgc atcacgataa gcccttgatg ccgggcaaca
    29641 aggagcggat gcatccgatt gtgcgggaaa atcttcaatt cgtgcaaagg accggacata
    29701 ggcaagcgga gcatatcccc cgactcaccc caaggtggcg ccaagaccta aagaggcctt
    29761 gagacccctg gacccaaggg tgactcgatg cggtggagtg cagacaacca cgggggatcg
    29821 tcttcgtttg aggttcgggg atgaggggct ccttggacgg atcagcacac cccgacctca
    29881 atgggttccc cggggagtgc gtccacggat ctccggccca accgtgcggg agtccacggt
    29941 cgcctgcgcg gcggtcgtcc cccgggatac ggacgaccct ccccaacaac acaccctctc
    30001 gtatacctga acctctttgc atcctgcatc gagcgatttg accgggacgg atcccgtctc
    30061 ttcgttcccg cgcagacggg aatcggatcc ggaaagagcg tgcatacccg ctgaatcccc
    30121 gccttcgcgg gcatgacgcc cggaggttct cggaatcgct caacccaggt ccataggagt
    30181 ccaaagcgag tgccgcgacc ggaccggcca tgaggtgacg accggcatcc tccgtatccg
    30241 tgcccgaatg catctgtttg aaaagcacac ggcaaaattt acttgacatg atcaatgaca
    30301 tactcaacaa tatcctcccg aggagatcac agtggccgac cttgccgaca gactccgcca
    30361 caaaggggat gagccgcact tgctgcgcga agtgatgcgg ctccatcatg ttcttttgag
    30421 cgttttcagc cgacaggtgg ggatgccggc ctcgaggctg gcgctgatgc gctttcttgc
    30481 aacccgccat tccgaggaca ttggaatcat ggagattgcc cggtgcctgg gagtgaaccc
    30541 ggcagcggtc acgcgtcagg taaaggagat ggaatcggag ggactcgtcg accgtgttgc
    30601 cgatgccagg gatggccgac gcagccatgt gaagctttcg gaggagggat tacgagtctt
    30661 cgaaagaatt catgaacgcg ctcatgaatt cgagcgggaa ctcgggtcgc atatcagcag
    30721 cgaagacatg gccacagcgg ttcgggttct gtcccagttg cggggagatc ttgaaaacct
    30781 gcgatgaagg agaacctcga gaaatgaaaa ctaccgagaa aagatcgttc aatctgcggg
    30841 gattcatcgt cctcacagcg accgtcaccg gtctggggct gccgatcacc ggctgggcca
    30901 accacctgca ccggatggaa ccatggatct cctcttcaca acacgcctgg atggcggctc
    30961 ataacgtcct gggaattctc ttcctggtgt ccgcggttgc gcatgccgtt ctcaatcgcc
    31021 gcatcctgtt caagtacatc cgaggccacg ccgcttgtcg gagagtaggc agagaagccg
    31081 tgggtgccgc actcctcgtg gccgtcctga tgtttgtcgc ggtgggccac gcttttcact
    31141 agggaccctc cgatccaagc ccagcgagga ggtttcatgc agatcgataa cga
//
LOCUS       DAOOVC010000033        31052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_984_length_31052_cov_6.203536, whole genome shotgun sequence.
ACCESSION   DAOOVC010000033 DAOOVC010000000
VERSION     DAOOVC010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 31052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31052
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_984_length_31052_cov_6.203536"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1694
                     /locus_tag="PLM79_06520"
     CDS             369..1694
                     /locus_tag="PLM79_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV85998.1"
                     /translation="MAAASAPDKVPGRAWVVTFAGTAVNLCLGILYAWSVWAKALVDE
                     KNVGKLMTGVNEGWEYLNNAQAATPFSLCVIMFALLMIPGGRIQDKYGPKVGATVGGL
                     FLAAGCILSGLMKSYAGLIIGFGILGGIGMGIGYAAPTPAALKWFGPHKRGLVAGLVV
                     GGYGGAALYIAPLAKYLIDTMSISGSFIALGIFFAVVVIIAGQLLSWPPAGYVPPAAP
                     APKVASATPVKPTLTVVDWMAGDMVKTWQYYALVLMFIGTTQSGLLIIANAAGILATT
                     AKDIPFFAANAWILVSFGGLVNALGRVGTGMYSDAIGRANAYALNCILSAVCLFAMPA
                     ITRSGSVLLLFIAVGIAYWQYGGGLALLPSFTADFFGPKNLGFNYGLVFIGWGLGFFM
                     ARLGGTIKDVTGSLDWAFYLSGLVLVAAVVISRIAKRPSSAQELATAKG"
     assembly_gap    2561..2660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2661..3365)
                     /locus_tag="PLM79_06525"
     CDS             complement(<2661..3365)
                     /locus_tag="PLM79_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV85999.1"
                     /translation="MPRYAVLSDVHANLEALEAVLQDSAAQGTDGLVHLGDLVGYNAN
                     PRECLELLREKCLRSIQGNHDRGIFDPETAFEFNFYAREALDYSRSRLGEEDIRYLTT
                     LPPTDLLDDRVLLCHGSPHSVFAYILDYGEARRAFQTLRKEYPAVRLCLYGHTHIPEV
                     WTLDEKEKLAAPSWIEPDLVMRGDRIYLVNPGSVGQPRNGDPRAAYLVLDTDRGSLNL
                     RLVPYDIASAREKILKA"
     gene            complement(3374..6097)
                     /locus_tag="PLM79_06530"
     CDS             complement(3374..6097)
                     /locus_tag="PLM79_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV86000.1"
                     /translation="MHKPPHNRQFRTGPHRRRRTSEEVPPKDSGPFRATVHRNLRPVL
                     DRIKVPEPVPFVPDPFQTQALETLEESDVLVTAPTGAGKTYIAVEAIRKVAGSGGRSW
                     YASPLKALSNAKYEEFSDIFGRENVGILTGDRKENLQASIIVGTTEILRNQLYDTMHR
                     GEDLDVDLVVLDEAHYLGDQDRGVVWEEVLIYLPARVKLLLLSATIQNAQEICDWLEW
                     LRQAPCRHVSAFERPVPLYPLFLFPSGELCPLGTRRGLFSKIRTVNSRSLSRSVLPTI
                     PKVMAVLRQFNLLPAIFFLKSRADCDKAITLGNLASPSKNRPDGRRFQNRVKNLLDTY
                     PFLRRHQQLSILRNARVGAHHGGQLPHWKLFLEKLMQDGYLDAIFSTSTVAAGVNFPA
                     RTVVIFQSDRFNGREFVQLTATDLLQMTGRAGRRGMDEIGFVCVVPGTYQDPQLVHDL
                     LKSPPDPIVSQIRVNFSMVLNLLLSHSPQEIHSLFASSLATYQNLSKEAEAREEARVA
                     EEELGSWVPEMACGSPERLREVRPRALALAERLRKSRKAFKRQVSSSPFFSLLQPGRV
                     FFSRRGTPYVAVADPQTRHQLVEAVRLAPPVRFRKGRVRTHRLHFRQIHGMGEVLEVL
                     PAFKDREAWEELLTRAEEQPFGEQAMEGFSGDAPGSIRAASRELDELLEEKASLPCDR
                     CALYGPCLKGTSHPFAACLQRDLEVQSRMETAHEELWRSFLKHFEFLRREGYVEESGH
                     LTEDGLWASNLRLDQPLLISECIRSGLFPTDDPDLLAALIAPFVMDRERLGDMQLSTM
                     VYKFPDLAKPFFKMLQNLQPLRENLNSAGFSTPPLAFWTMVTVYHWARGMSWEEIREL
                     AQMDEGDLAMVILRTADHLRQIESLSETHPRLAASATRAIERILREPVLVE"
     gene            6732..8063
                     /locus_tag="PLM79_06535"
     CDS             6732..8063
                     /locus_tag="PLM79_06535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOV86001.1"
                     /translation="MKIAIIGTGYVGLVTGACFAEFGHEVTCIDIDESKISLLQQKQV
                     PIYEPGLDMLVRKNLDARRLRFTTDFVPGIPEAQVVFIAVGTPASRRGDGYADLSYVY
                     DAARRLAPHLEGYTVVVNKSTVPIGTARQVARILSEVNPEADFHVASNPEFLREGAAI
                     NDFMRPDRVVLGVDSPRAEEVLKAMYRPLYLIETPFVITNIETAELIKYAANAFLATK
                     ISFINEIANLCEEVGADVQGVAKGMGLDGRIGRKFLHAGPGYGGSCFPKDTQALLRIA
                     QEHGVACRIVESVVEVNAAQKARMVRKIRKALGGDEAGKTVAVLGLSFKPETDDIRDA
                     PSITILSTLLEHGAAIRAHDPQAVEEARKVLPQATYCSDPYEACRGADAVVLMTEWNE
                     YRALDLDRVKSLLKEPVFIDLRNVYKLPSMKEAGFRYYSVGRPCVGDGLVG"
     gene            8182..8364
                     /locus_tag="PLM79_06540"
     CDS             8182..8364
                     /locus_tag="PLM79_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86002.1"
                     /translation="MDRWVYWLSLVGGVLLFLHGIGKLYREGDWTLLVITLLIVGFSL
                     SGLLKGSGGKGKGNGP"
     gene            8454..9944
                     /gene="lysS"
                     /locus_tag="PLM79_06545"
     CDS             8454..9944
                     /gene="lysS"
                     /locus_tag="PLM79_06545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOV86003.1"
                     /translation="MDDLNLVIRERIQKAQEMEAAGIPLFPNGYAVPHRIGEVLAAYE
                     DHSGEALEAEAPTFTIGGRILAVRSFGKSVFMHVRDSGGKIQVYLQQNRLGKEAYARV
                     KELDIGDIIRVHGTLFRTRTNELTLLAAEFELLSKSFRPLPEKYHGLKDIEIRYRQRY
                     VDLIVNEEVRQTFFKRARIVQIIRGFLNERNFLEVETPMMQPIPGGATAKPFKTFHNA
                     LGMELYLRIAPELYLKRLLVGGLDRVYELNRNFRNEGISTQHNPEFTMLEFYMAYATY
                     EDLMDLTEELFSAVAAGVNGGSLQIEYQGDVVDLTPPWPRYRLLDALTQIGGLPASAL
                     GDPGQTKELAREMGISPEQHEGHGKLLTKLFDLTVEPKLVQPTFITHYPIEVSPLSRR
                     NEQDPSVTDRFELFIAGREMANAFSELNDPRDQRQRFLQQIEARRAGDEEAHLMDEDY
                     IRALEYGMPPAAGEGIGIDRLVMLFTDSPSIRDVILFPHLRAEKKP"
     gene            10030..11268
                     /locus_tag="PLM79_06550"
     CDS             10030..11268
                     /locus_tag="PLM79_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOV86004.1"
                     /translation="MPMTFEFFVSLRYLLAKRRQTFISLITIISIAGVAVGVTALIVV
                     LAVMNGFQEDLRGRILGITSHVVVGNFNGTIPNYETIVLEVKNARGVLAATPFTYSQV
                     MISSGKNVSGAILRGIDPKTAPEVIQLKKNLIRGNLEDLAPSDVSTLGRNIILGVELA
                     KNLGVQQGEWVTLIAPTGRLTPMGQNPKSSLFHVVGIFQSGMYEFDNTLAYIDLSAAQ
                     QFLNIGDTVTGIEVRVKDIYDAGAVAESLRSRLGYPFWVRDWMQMNRNLFSALKLEKV
                     VMFIILTLIVLVAAFNIVSSLIMMVMEKTRDIAIMKAMGATTARIRKIFILEGCMIGV
                     SGTLLGLMGGFGLCAVLKRYRFIELPPDVYYLSTLPVDLKALDVFAIALSAILISLLA
                     TIYPSRQAAKLDPAEALRYE"
     gene            11261..12019
                     /locus_tag="PLM79_06555"
     CDS             11261..12019
                     /locus_tag="PLM79_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86005.1"
                     /translation="MNSHPNGQPIEVHAMSLSKIFGSGVQSIELFRDLDLIIRKGERI
                     AIVGASGAGKTTLLHILGTLEKPSTGKVLYGGNDIFLWDEKDLAQFRNRNIGFVFQFH
                     YLLPEFNALENVMMPGLIAGLARKEVRTQAEEILERLGLSARLEHRVGELSGGEQQRV
                     AVARALLLRPKLFLADEPSGNLDSRTGRKLHELLVSLNEELGLTMVIVTHNPELASMM
                     HRTLRLVDGQLRPQPSFGLSCMAPSPINSQRDGE"
     gene            12214..14868
                     /gene="bamA"
                     /locus_tag="PLM79_06560"
     CDS             12214..14868
                     /gene="bamA"
                     /locus_tag="PLM79_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOV86006.1"
                     /translation="MRKLCILLFLLTFIAPVGWAQGPSARVGVLPFVMHSRESLPDAQ
                     RQVQELLIRQLSEEGVRAVPVGEVDKVVKPRDAVQTVEQGRSYGHRLQADYVVIGSIN
                     VIGGSVSLDAKLVDVSGRKKTEPLFAEERGLENLASTTKSLIQQMTVHVLAKAVIADV
                     QVRGNDRIEAEAIKANVKSKKGEVLNREQVSEDIKAVYKMGYFEKVEVEMADGPGGKI
                     LTFVVEENPTIAEVKPRGNKKIKEKDIMAAITTKSYMVLQKNVVAEDVQKIIKLYHQK
                     GYFDVDVQSKVEFPKDPRRATVIFEIKEQKKVLIQKIGFRGNKKYSARRLRSVMQTKE
                     KNFLLSLFTDRGILQKDMLATDVDRLTVFYHDQGYMDAKVGNPDVTQKSDGFYIEIPI
                     DEGQRYKVADVKITGDLLETSADKKTAKELKTRAKDYFSREKVRKDIDNISKAYMDEG
                     YALAQVNPEVKRDPASHTAEVVYNVSKKDKVHIGKIYVTGNTKTRDKVIRRQLKLAEG
                     DLFNATNLEKSLLNLKKLDYFEEVEITPVETAQGDIMDLHVKVKEKLTGTISVGGGYA
                     SQDGLFTSAQIQQRNLFGRGQTIGIRGYLSEEAQRYVLSFTEPYLFDMPLAAGFDIFN
                     WIRDYDTFTQDSYGIRLRTGYPFGSWSRMSLWYTIEEDEVTDISRNASKYIKDQRGKY
                     LKSAFTLGFERDTTDHPFLPTRGSLNTLMSEFSSSYIGSDSDYVRTEIQSGWYHPLFW
                     KFIGFAKAQYGYIFPLDDRSKAVPIYERFFLGGINNLRGWKWADIGPRDSHGDVVGGY
                     HYMVLTGEILFPLVDKYGIRGVTFFDAGNSYREKEDIDFSSFRTDAGVGLRWNSPMGP
                     LRIEWAYNLDPKPKEDNYQWQFSAGAFF"
     gene            14881..15432
                     /locus_tag="PLM79_06565"
     CDS             14881..15432
                     /locus_tag="PLM79_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOV86007.1"
                     /translation="MKGKWSRRLVLWTLMGFTLNLMAVSAWAATGKVGYFDLQVILDQ
                     SQAGKQAKQEFTAEKDRMKATMDQKANAFKQAKEEYDRKKGVMDESAKKKKDKELADL
                     QQEAERALMEANTKLNKLSSDLMAPIVDRIIEIAKKIGKDDKYDYILEAGKGGIVYAN
                     EKEDLSKKIIEALDKSPIPARKP"
     gene            15819..16886
                     /gene="lpxD"
                     /locus_tag="PLM79_06570"
     CDS             15819..16886
                     /gene="lpxD"
                     /locus_tag="PLM79_06570"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOV86008.1"
                     /translation="MQGAQDPTRQKWFSLGELSRVLGATLRGDAEIRVRGLRPLEVAG
                     PDEISFLTHAKYVSLLSRCKAGALIVSSSFQDLEYPLLISDNPYLTMARAAQLFAPVP
                     EATPGVHPTAWVAPEAQVDPTASLGPLCQVGTGSRVGAGTILHGGVVVGNEVEIGNGC
                     LLYPNVAVLDRCKIGHNVIIHAGTVIGSDGFGFAQDEQGRHVKIPQSGIVQIDDDVEI
                     GSNCSIDRATFGRTWIQRGAKIDNLVMIAHNVVVGEDSILVAQVGISGSTRIGKHVVL
                     AGQVGVAGHLEIGDGARVGAKSGVPHSIKPGEDMLGIPAVPSKQFFQTFANLQRLSQF
                     KEQLRQLQNRVRQLEETMPGE"
     gene            16890..17333
                     /gene="fabZ"
                     /locus_tag="PLM79_06575"
     CDS             16890..17333
                     /gene="fabZ"
                     /locus_tag="PLM79_06575"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOV86009.1"
                     /translation="MEQDILQILKVLPHRYPFLLLDRVLEMSSQHLVGLKNVTINEPH
                     FQGHFPAEPIMPGVLIVEALAQAGGVLAFSLVPQHMGNAVYFMGMDKVRFRKPVRPGD
                     QLILKLNVLRQRGSVFKMKGEAFVDDQLVAEAELLATIDPGKSEE"
     gene            17399..18199
                     /gene="lpxA"
                     /locus_tag="PLM79_06580"
     CDS             17399..18199
                     /gene="lpxA"
                     /locus_tag="PLM79_06580"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOV86010.1"
                     /translation="MEIHPTAIVSPKAELGENVKVLPYSIIGSQVTIGSGSVIGPHAI
                     VDGRTTLGRNNQVFPFATVGYPPQDISYKGEDTECVIGDGNIIRESVTIHRGTAKGGG
                     ITRIGNGCCVMAYVHVAHDCNIGNHVILSSGIGMAGHVEIHDHAIVGGMVAIHQFVRI
                     GSFAFVGGMSGLRMDIPPYMLAHGAPAKLYGPNLVGLRRHKFPMQTVQALKRAYKILF
                     RSTLTVKDAVDKIREEIEPLPEIEKLLQFMQEDSRRGVTRWGSDEAVE"
     gene            18183..20114
                     /gene="lpxI"
                     /locus_tag="PLM79_06585"
     CDS             18183..20114
                     /gene="lpxI"
                     /locus_tag="PLM79_06585"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HOV86011.1"
                     /translation="MRPSNDPLEHPKERIGLIAGAGQFPLLFAHAAYQAGFRVVAVGF
                     DGETHPSLPKYVDEFHWQNLGQFNRLIQTFKKAGVRQAVLAGSINKTKLYARIRPDWR
                     AVKFLSKLRNKKDDFLLRAFSRELESEGIQIAPSTIFLPSLLAPEGIFTRRRPNRQEE
                     SDILFGWQTAKALGHLDIGQCIVVRNQAVLAVEGMDGTDATILRGGHLCKEGAVVIKV
                     SKPSQDLRFDVPAVGYDTIATMKRVKARVLVIEAGKTLIFDREKMIEAADEAGITIQA
                     LRDEKALEEVVDEGRDSVRAYLNRLDRSRSPSVTGPPPVLVREPHEKALKVAVVGVGY
                     LGRFHAHKYAGFRDANLVAVVDIDPEKARQVAQEVSSTPLADYRELLGQVDAVSIVTP
                     TPQHFSIASDFLAAGVHVLLEKPMTQSLAEADELIRLARENNCFLQIGHLERFNSAFQ
                     AVLPRLNRPMFLEAHRLALFNERGLDVDVILDLMIHDIDLVLHMVRSPLKRIRASGVS
                     VLTSVPDIATVRMEFENGATANLTASRISLKNQRKMRIFQENTYLSADYAEKRAYVVY
                     REQEWDEAGFPQLSIEELEIQERDALGEEILSFLDCVRAGKPPWVDGVQGRRALAVAL
                     DISRQIEEQLEKGRGEGGS"
     gene            20128..>20434
                     /locus_tag="PLM79_06590"
     CDS             20128..>20434
                     /locus_tag="PLM79_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOV86012.1"
                     /translation="MSRTIFVSAGEASGDLHGSRLAAALRELEPDIRITCLGGPLLEQ
                     AGARVLVHNREIAVVGIFEVLRHLKDLWRAWRMVREHILRERPDPVVLIDFPDFNLLL
                     "
     assembly_gap    20435..20534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20535..21428
                     /gene="lpxB"
                     /locus_tag="PLM79_06595"
     CDS             <20535..21428
                     /gene="lpxB"
                     /locus_tag="PLM79_06595"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOV86013.1"
                     /translation="ERPDPVVLIDFPDFNLLLARLARRRGLRVFYYISPQVWGWRTGR
                     VRTIRRLVDRMAVILPFEEEFYRRHGFKVHYVGHPLLDVLAHAPSRSRAQERYRTGEA
                     GPVVGLLPGSRRSEIRSLLPILLETAALLRQRFPHILFLLPVAPGLHREEIEERASEA
                     SLPLRVVSGDTYGVIRACDLVLAASGTVTLETAILGTPLILFYRVSNLTFNAGKHLVR
                     VPWAGLPNLIAGKMIVPEFIQDRARPDLIAAEAASFLDDPARLEAQRRELEAIQHQLG
                     EPGVARRTARLVLDLRREISS"
     gene            21484..22074
                     /locus_tag="PLM79_06600"
     CDS             21484..22074
                     /locus_tag="PLM79_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86014.1"
                     /translation="MNGKVAAVKTVLSFILIVVVLAWGIENRQAGAQSFKLGPRQERK
                     ASSPPASEPGKKAESEKSEPATPAAPGETPKEEPGKASGESAPPNTDMKQAGAELREP
                     REEGPPGIKWADNNQEQRCLSMLAELRKEFLQARFYSIQGDACSTADHAKSFLKIVND
                     IQPQCPENFLEKKGYRPVILRNLNWLYKLGTDRCLQ"
     gene            complement(22081..26004)
                     /locus_tag="PLM79_06605"
     CDS             complement(22081..26004)
                     /locus_tag="PLM79_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV86015.1"
                     /translation="MDDRGPPTQPPACSSWIHRVIWFCLERKILVLLFTLFVIVGGIV
                     VAPFDWKLRGIPRYPVPVDAIPDISENQQIVYTEWPGRSPRDIQDQVTYPLTASLLGL
                     PGVKAVRGYSYFGFSNVYVIFKEDIEFYWSRSRLLEKLSSLPPNTLPKGVQPTLGPDA
                     TALGQVMWYTLEGRGPSGKPAGGWDLDELRTLQDWYVRFGLLSAEGVSEVASVGGYVR
                     EYQIDVDPNLMRIFDVKLEDVVAAANRSNLDVGAAGIEINKVEYIVRGVGFVKGPTDL
                     ENALIKVTDDVPVFIRNVAYVHYGPAQRGGALDNGGAEAVGGVVTARRGANPLETLNN
                     VKEKIREISAGLPSKVLADGTVSRVEIVPFYDRSGLIYETLGTLSDAVVQETLTAIVV
                     ILLVILHLESSLLISSVLPLAVLLSFLGMKLFGVDANIVSLAGIAIAIGTLDDMGIVI
                     CENILKHLQAADPGESRIRVIFRGASEVGGAVLAAAAATIISFLPVFALDGAEGKLFK
                     PLAWTKTFALLGSAVVAVTVIPAMAHLLFFRERDKKASGRVLSAILIPAGILLMAFVH
                     GWLGGFVLLVGLYKTFLRWIPEAWKSRLKGAGSILAAALVALLLTRDWLPFGPEKGLF
                     LNFLFVAFLIGGLLSSFLLLMHYYRPILGWCLDHKGPFLLLPAAMLLLGATIWLGFPT
                     VFGWLPSFLKQTAPLVYVAHKFPGMGEEFMPPLDEGSFLYMPITMPHASIGEGLDIIQ
                     KQDKRIRAIAEVESVVGKLGRAESPLDPAPASMIETLIQYRPQYLSDRDGRPLTFRYS
                     SRENDYHRDIEGNPVNAPDGMPYVVRGRFLRDSANGLLPDPYGKPFRLWRPPLDPTLN
                     PGRRAWKGIRNPDDLWDAITAAANIPGSTQAPKLQPISARLVMLQSGIRASMGVRVKG
                     PDLPSIENACAQIERYLREVPSIEPKSVIGDKIIGKPYLEIRMDREALAQYEIDLEQA
                     QRVISVAVGGDRIMTTVEGRERFPVRVRYLRELRDDFESIGKILVPSANGSQIPLMQL
                     ADIRYVRGPEVIRTEDTYLVGYVLFDKKPGIAEVEAVEHARDYLNFKVESAELQIPKG
                     VGFSFTGSYENQVRSENRLRVILPLVFAVLFVIFYLQFKSVGTALLIFTCIPVAWAGG
                     FILLWLYGQPWFLNFELLGTNLREVFRVHPIFLSVAVWVGFLALFGIAADDNIVMATY
                     LEERFAGRSFSSIREIRETTIEACRKRIRPCLMTTATTILALLPVLTSSGRGSDIMLP
                     MAVPSFGGLTFEVVTMLVAPVLYCAGKEFQFRRKLSREFIPPGAR"
     gene            complement(26040..27740)
                     /locus_tag="PLM79_06610"
     CDS             complement(26040..27740)
                     /locus_tag="PLM79_06610"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV86016.1"
                     /translation="MKKLFETFFSGPPRYKVAVAVVLSFVVGFGIRSLGSLTHGDPLE
                     TAPPAQRLAGKPHVHPGEKNGVRSFLCPMMCIPPREASGLCPVCGMELVPASSMEATG
                     SGTSLKLSPEAVALAELQTAPVERKHVSARIRLYGKIEYDPAHVNYVTAFMPGVIDRV
                     YVKRPGQFVRWGAPLFSIYSSDLYYTQQQLVEAMRFVPSFLAWQEGQPYIARDAMVQE
                     RKGKEDPQHRSPEVEKAMQTVEAVRHKLTILGMPKRDIDELMKVGEPNGIATVYAPMY
                     GQVVEVNATEGSYVNTGTSLFTLADPKYVWARLDAYEVDFPWIRLGQKVSFQVDAYPG
                     ETFEGDIVNINPAFKTKTRTFEIGVILPDTGGRLKSGMLVRSIVHAEVTAEGRVAGDE
                     PHRPENAPLVIPASAPLITGKRAVAYVAVPGEPGAFEGREILLGPKTRDHYVVLDGLE
                     EGEQVVVNGSFKVDSAVQILARTSMMSLEGGHTALNYQPSGGSQALHDEYWSQRTKSR
                     LRTGAESETSLQDLKAAGEGSDVGHGEGRHPSRAKIQRRRPGTYGDTTQPTPAPRSPW
                     "
     gene            complement(27795..28247)
                     /locus_tag="PLM79_06615"
     CDS             complement(27795..28247)
                     /locus_tag="PLM79_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86017.1"
                     /translation="MQTHAPLNLRVMKGIPALPVLAAVFYCFVFCLTLVAPASAATEK
                     DLGIQIVALRMTASDHFVDCRYRVVDAEKAKPFMARKTEVSLIDQATGKKLPVSMTRL
                     GSMRSTSTKPRTDKVYNILFRNLNKTVVSGNLVTLVSGDAKVENLKVE"
     gene            complement(28486..28698)
                     /locus_tag="PLM79_06620"
     CDS             complement(28486..28698)
                     /locus_tag="PLM79_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86018.1"
                     /translation="MKREGYTGGLAVFRKPSVRASAIQPDDLPCSILAIFSRQPWASM
                     AASRIRPQVGTVGKGDTTPLYRPTTR"
     gene            complement(28885..30216)
                     /locus_tag="PLM79_06625"
     CDS             complement(28885..30216)
                     /locus_tag="PLM79_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOV86019.1"
                     /translation="MMYRKTGNNRVRGWILALFLLTVSAPAAHADITFNLRAAPVNLT
                     MADGVVVPAWGYALVSTNEDGTTTPGDDVVKVPGPTLVVPPAQNRVTVSLINQLPEET
                     SLHILGQVLSNNSGPVWTEGHTTVTYTGSRPAENYTARVRSFSHETAGSGGVGSYRWD
                     NFRPGTYLLSSGTNPAKQVQMGLVLPMVKDAAPGQAYTGVAYDKQLVVLFHEIDPVIQ
                     RAVADGNYGYGKTIPSSSHRAPKYSLINGKSYPDASLNPINASINPITPGQKILFRFV
                     SACLDTHIPQFLGTYLNAVAEDGNPYDFPRSQYAFELPAGKTIDAIFTATAGRIPIMD
                     ARLDVTNAGAPTPGGMIAFLGVTDTVTITKAVYNAAGQSLGVWATSNAPGGTAELSAS
                     TSTGGTPADLGQLIYRANFGEYREVFSGVAAKPDSVTLTSSAGGSATVAVP"
     gene            complement(30248..>31052)
                     /locus_tag="PLM79_06630"
     CDS             complement(30248..>31052)
                     /locus_tag="PLM79_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOV86020.1"
                     /translation="LIVRPGGGPDPAHHAYNHADSYFDQEYLYFITEMDPWIHRQVEF
                     TPASAVDTTKYFPTNWFINGRNWPDVMSPAFAKWLPTQPYNCVLRMHPGQKILVRLVG
                     GGRDIHPMHLHGNNFWVIAEDGRMFSSNPGVSGADLAWSDVTYKIAAGRTADAIWSWN
                     AEKLGWDIYGHAPGDPMEPGEYAPDHGKPFPVVLPTRDSMTYGPFYSGSPFLGAGGDL
                     PPGDPGLNANAGYFYMWHSHTEKELTSNDIWPGGLVSFMIIEHPNVPIP"
BASE COUNT         6140 a         9254 c         9692 g         5766 t
ORIGIN      
        1 tttcaatata ttattccatg gcgccacccc tcaaagggtg tgcgaaactt cagttgttat
       61 ccttggaaat ttgagcttca tctttctgcg gttctgcgtc attcattgaa cctaccccgt
      121 tctccttaga tgccctttgg tttagcggtt cgcattccta cccgagaaga ccccaagcat
      181 cggatccgga ccccgttgcg gtttcctttc taccggttcc ggacatggac ttgatccggc
      241 agtgatccca acgggttggc ttcgatcgcc ctgcccgcgg cgtgccgtgg agtggaaagg
      301 cagcttgggg ggttcctgga ccttcaccct ttcggtcgtt tggaaaggat cagcggaagg
      361 agacgaaaat ggcagcagca tcggcaccgg acaaagttcc gggaagagca tgggtggtca
      421 cctttgcggg gaccgccgtg aatttgtgcc ttgggattct ttatgcctgg agtgtctggg
      481 ccaaggccct ggtggacgag aagaacgtcg ggaaactcat gaccggcgtc aacgaaggtt
      541 gggagtacct gaacaacgcc caagcggcca ctcccttttc cctgtgcgtc atcatgttcg
      601 ccctcctgat gattcccggg gggcgcatcc aggacaagta cggccccaaa gtcggagcca
      661 ccgtcggggg actttttctc gctgctggct gcatcttatc gggtctcatg aagagttacg
      721 cgggcctgat catcggcttc gggattttgg gcggcatcgg aatgggcatc ggctatgcgg
      781 cgccgactcc cgccgccctg aagtggttcg gaccccataa acggggcctg gtggcgggcc
      841 tggtcgtcgg gggctacggg ggtgcggccc tctacattgc ccctctggca aaatacctca
      901 tcgataccat gagcatttcc gggagcttca ttgccctggg gatcttcttt gccgtggtgg
      961 tgatcattgc gggacagctt ctctcctggc ctccggcagg atatgtgccc ccggcggccc
     1021 cggctcccaa agtggcttcg gcgactcccg tgaagccgac cctcaccgtg gtggactgga
     1081 tggcgggaga catggtgaag acttggcaat actatgccct ggtgctgatg ttcatcggca
     1141 cgacccagtc gggccttctc atcattgcga acgccgccgg gatcctggcc accaccgcca
     1201 aggacatccc gttctttgcg gccaacgcct ggatcctggt ttccttcgga ggcttggtga
     1261 acgccctggg acgggtggga acgggaatgt attcggatgc gatcggtcga gccaacgcct
     1321 atgcgttgaa ctgtatcctt tcggcggtgt gcctgtttgc catgccggca atcaccaggt
     1381 ccggcagcgt gctgcttctc ttcattgccg tggggatcgc ttactggcag tacgggggcg
     1441 gactggctct tctgccttcc ttcacggcgg atttcttcgg acccaaaaac ctgggcttca
     1501 actacggtct ggtcttcatc ggatggggac tcgggttctt catggccagg ctgggcggca
     1561 ccatcaagga cgtaacgggg agcctggatt gggcctttta cctgtcggga ctggtgctgg
     1621 tcgcagcggt ggtcatatcc cgcattgcca agcgaccctc ttcggcccag gaactggcca
     1681 ccgccaaagg ctaagaggct agggggctcg gaggccgaaa ggcgaggcgg gttggtcagt
     1741 tggttggccg gccgggggct ggtggctgga ggctagggac tacaggccga aaagcccggg
     1801 gattcgaaac ctccccagcc cgcgcaagaa ccgatgggag ctccatggac agccattgtg
     1861 caaacggcgc cccttcccaa cccgcaacct ggggacgggc ggatcgaaag aagacccgtc
     1921 cgaaccgacc ttgtcaatct tccgcctttg tccacccggg ggaccccatc ggcccctagg
     1981 ccctgacaat ggtcagcgtg aaaaggttta ccgacggttt gacgaaggag gcccctcccg
     2041 tgaccgggag gggcctcaaa ccaggttgcg ctcaaggtcg ccctgacatg ctgagtgcag
     2101 atttcgagca aacggctaat cctgcgggtg ggtggtttcg gcttgaatgc aacatggtat
     2161 caaacgttgg tggatcaaat cgggcgggag acgggggagg ctgggaagca gtcaagaatt
     2221 cacacggcca cttggttccc gtccggagtc tgagagcaag gctgggaggc cgggaagcaa
     2281 gaaggctgga aagtggaggg gttgaaactc cggggggggc acgagcccgc agcggcggtt
     2341 gggttgcttg ctttggcggc agagcaagct tgcctcacca gtgtgcgtca cggggggagg
     2401 acaaagcgtg ggaacgggaa accgatgccc gccgggtccc agccggcagc atgccgaaat
     2461 gggcgtccgg cttcccagcc tttggcctct cggtgggctt cgcttcgctc cacccaccct
     2521 acacatctca gtttcccagc ttcttgccac ccggtgggct nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn tgccttgagg atcttttccc gggccgaggc gatgtcgtag
     2701 gggaccaggc gcaggttcag ggacccacgg tccgtgtcca ggaccaggta ggcggcacga
     2761 gggtccccgt tccgtggctg gccgacgctt cccgggttga ccaggtagat tcggtctccc
     2821 ctcataacca ggtcgggctc gatccaggag ggggccgcaa gcttttcctt ttcgtccagg
     2881 gtccacacct ccgggatgtg ggtatgaccg tagagacaca ggcgaacggc ggggtattcc
     2941 ttccggaggg tttgaaaggc gcgccgggct tccccgtagt ccaggatata ggcgaagacg
     3001 ctgtgggggc tgccgtggca caagagaacc cgatcgtcga ggaggtcggt tggagggagg
     3061 gtcgtgagat agcgaatgtc ttcttccccc aggcggcttc gggaatagtc gagggcctcc
     3121 cgggcgtaaa aattgaactc gaaagccgtc tcgggatcga aaattcctcg atcgtggttg
     3181 ccctggatgg accgcaggca cttttcccgg agaagttcga gacattcccg ggggttggcg
     3241 ttgtagccga ccaggtctcc caggtgcacc aggccgtccg ttccttgggc ggccgagtcc
     3301 tgcaggacgg cctccagggc ctcgagattt gcgtgaacgt cggacaggac ggcgtagcga
     3361 ggcatttccc gtgtcactcc accaggaccg gttcccgcag gattcgttcg atggcccggg
     3421 tggccgatgc cgccagtctc gggtgggttt ccgaaaggga ttcgatctgt cgcaggtggt
     3481 ccgcggtcct caggatgacc atggcgagat ctccctcatc catctgggcg agctcccgga
     3541 tctcctccca ggacatgccc cgagcccaat ggtacacggt caccatggtc cagaaagcca
     3601 ggggcggggt gctgaacccg gccgaattga ggttttccct caagggctgg aggttttgca
     3661 gcatcttgaa aaacggcttg gccaggtccg gaaacttgta caccatggtg gaaagctgca
     3721 tatcgcccag gcgctctcgg tccatgacga agggagcgat gagggcggca aggagatcgg
     3781 ggtcgtctgt ggggaagagt ccgcttcgga tgcattcgga gatgagaagg ggctgatcca
     3841 gacgcaggtt ggacgcccac aggccgtctt cggtgaggtg accggattcc tccacgtagc
     3901 cttcccttcg aagaaactcg aagtgtttga ggaaagatcg ccagagctct tcatgggcgg
     3961 tttccatgcg gctttggacc tcgagatccc tttggagaca ggcggcaaag gggtgagacg
     4021 tccctttgag gcagggtccg tacagggcgc atcgatcaca gggaagggag gctttctcct
     4081 cgaggagttc gtccagttcc cgggatgccg cccggatgga acccggggcg tcccccgaaa
     4141 aaccctccat agcctgttcc ccgaagggtt gttcttccgc ccgggtcaac agttcctccc
     4201 aggcctcccg gtccttgaag gccggaagaa cctcgaggac ctcccccatg ccgtggatct
     4261 gccggaaatg gagccgatgg gtgcggaccc gtcccttccg gaaacgcacg ggtggggcca
     4321 gtcgtaccgc ttccaccagt tggtgccggg tctggggatc cgccaccgcc acgtaggggg
     4381 tgcctctccg gctaaaaaag acccggcccg gttgcagcag gctgaagaag gggctcgagg
     4441 atacctgccg tttgaaggcc ttccgggatt tgcgaagcct ttccgccagg gccagggccc
     4501 gggggcggac ctcccggagc cgctccggcg acccgcaggc catttccgga acccaggagc
     4561 ccagttcctc ctcggcgacc cgagcttctt ctcgggcttc ggcttccttg ctgaggttct
     4621 ggtaggtcgc cagggaggag gcgaaaaggc tgtgaatctc ttggggagaa tgggaaagca
     4681 acaggttcag gaccatggaa aaattgaccc ggatctggct gacgatgggg tccggaggag
     4741 acttgagaag gtcgtggacg agctgagggt cttggtaggt tccgggaacc acgcacacga
     4801 acccgatctc gtccatgccg cgccgcccgg cccgcccggt catctgcaaa aggtccgtgg
     4861 ccgtcaactg gacaaactcc cggccgttga accggtcgct ttgaaaaatc accacggttc
     4921 gagcggggaa attcaccccg gccgccaccg tggaggtgga gaagatggcg tccaggtagc
     4981 cgtcctgcat gagcttctcg agaaagagct tccaatgggg gagctgcccc ccgtggtggg
     5041 ctcccacccg ggcgttccgc aggatcgaga gctgctggtg tcggcgcaaa aaggggtagg
     5101 tgtccaaaag attcttgacc cggttctgga accggcggcc gtcggggcga ttcttggacg
     5161 gggaggccag gttccccagg gtgatggcct tgtcgcagtc agcccgggat ttcagaaaga
     5221 agatggcggg caagaggttg aactggcgaa gaaccgccat gaccttgggg atggtgggca
     5281 gcacgctgcg ggagagggac cgggagttca cggtccggat cttggaaaag aggccccgcc
     5341 gagtgcccag ggggcacagt tcccccgagg gaaaaaggaa aagcggatag aggggcaccg
     5401 gccgctcgaa ggccgatacg tggcggcacg gggcctggcg cagccactcg agccagtcgc
     5461 agatttcctg cgcgttttgg atggtggccg aaagaagcag gagcttcacc cgggcgggca
     5521 ggtaaatgag gacttcttcc cacaccaccc cccggtcctg gtcccccagg tagtgggcct
     5581 catccaggac caccaggtcc acatcgaggt cttccccccg gtgcatggtg tcgtaaagct
     5641 gattgcggag gatctccgtg gtgccgacga taatggaagc ctggaggttt tccttgcggt
     5701 ctcccgtgag gatccccacg ttttcccggc cgaaaatgtc cgagaactcc tcgtacttgg
     5761 cgttggagag cgccttgagg ggagaggcat accaggagcg gccgccggac ccggcgactt
     5821 ttcggatggc ttccacggca atgtaggtct ttcccgctcc cgtgggagcc gtcaccagga
     5881 cgtcggattc ctcgagggtc tcgagggcct gggtctggaa ggggtccggc acgaaaggaa
     5941 cgggttcggg caccttgatg cggtccagca ccgggcgaag atttcggtga acagtcgctc
     6001 gaaagggtcc cgagtccttg gggggcactt cttcggaggt gcgcctgcga cggtgtgggc
     6061 ccgtgcggaa ctgccggttg tgcgggggct tgtgcaaatc tgtcctctct aagccatcgt
     6121 atattttgat cattcctaat aacacccgga cgccccgggg gcaagaggaa aggcgggtgg
     6181 gaagggcgag gggcgacaaa ccaggggatg gggtgcccaa ggggtcaggt ctccgtttgg
     6241 ccgacctcag gggtcgtgtg atccccgggg tcacccatca aaaggtttgg gtcaggtctc
     6301 cgtagggcgt gtcccggggt gcagaggggt cggggcgacc ggccgatcgc ccctatcagg
     6361 ccgtggggcg caatcccgct cttgtgcacg ggacggttct cgaccccatg ggtggatttc
     6421 ctctcgggac cggttccggg aggtatcggc catggggggt cgatccccgg caaatggttg
     6481 cgaaccctcg ggattcgttc cgagacggcg atctatcgcc gcctgatcgc agatgtggat
     6541 aaagggcagg gcgaggggct gggaagcggt gtagggtggg cttcgcttca cttgacccac
     6601 cccacacccg tcccggcgcc cctcgccctc ctcgcccctt gtaaccccgc gcataagctg
     6661 ttacagtctc gccgatttgg gacttgttgt cagacaaccg tcaacctgcg ggacgaggaa
     6721 ttggaagaac catgaagatt gcaatcattg ggaccgggta tgtaggactg gtaacggggg
     6781 cctgcttcgc ggaattcggg catgaagtga cttgcatcga tattgatgaa tccaagatct
     6841 ccctgcttca acagaagcag gtccccattt atgaacccgg cctcgacatg ctggtccgaa
     6901 agaacctgga tgcccggcgg ctccggttca ccacggattt tgttccggga atccccgaag
     6961 cccaggtggt tttcatcgcc gtgggcaccc cggcttcccg ccgtggagac gggtatgccg
     7021 acctcagcta cgtgtatgat gcggctcgcc ggctcgcgcc ccacctggag gggtatacgg
     7081 tggtggtcaa caagagcacg gtccccatcg ggactgcccg ccaggtggcc cggatcctct
     7141 ccgaggtcaa cccggaggcc gatttccatg tggccagcaa cccggagttc cttcgcgaag
     7201 gggcggccat caacgatttc atgcgcccgg accgggtggt gctgggagtc gattcccccc
     7261 gggccgaaga ggtcctcaag gccatgtacc gacctctgta cctcatcgag accccctttg
     7321 tgatcaccaa catcgaaacg gcggagctca tcaagtacgc ggcaaatgcc ttcctggcca
     7381 cgaagatcag cttcatcaac gagatcgcca acctgtgtga agaggtcggg gcggacgtcc
     7441 agggggtcgc caaggggatg ggcctggacg gccgtatcgg caggaagttt ctccatgccg
     7501 ggcccgggta cggggggtcc tgctttccca aggacactca ggctctgctg cgaatcgccc
     7561 aggaacacgg ggtggcgtgc cgcatcgtcg aatccgtggt cgaggtgaac gcggcccaga
     7621 aggcgcgcat ggttcgcaaa atccgcaagg ccctcggcgg ggatgaagca ggaaagaccg
     7681 ttgccgtcct gggactgtcc ttcaagcctg agaccgacga catccgggac gcaccctcga
     7741 tcacgatcct ctcgactctc ctcgaacacg gcgccgcaat tcgcgcccac gatccccagg
     7801 ccgtggaaga agcccgaaag gtccttcccc aggcgaccta ctgttccgac ccctacgagg
     7861 cgtgccgggg agccgatgcg gtggtgctca tgacggaatg gaacgagtac cgggccctgg
     7921 atctcgaccg ggtaaaaagc ctgctcaaag aaccagtgtt catcgatttg agaaacgtgt
     7981 acaaactgcc gtccatgaaa gaggccggct ttcggtacta cagcgtcggc aggccttgcg
     8041 tgggcgacgg gctggtcggt tagttggttt gttggttgac aagggccgag gggctggagg
     8101 ccgggggctg ggaaggtagg gaggctggga gggcgggggt ccgagggacg ttgtctgcag
     8161 cagaagggag gaaacggggg catggatcga tgggtgtact ggctaagcct ggtcggaggg
     8221 gtcctgctgt ttctccatgg aattggaaag ctctaccggg aaggggactg gacgcttctt
     8281 gtcattactc tgctcatcgt cggattcagc ctgtccggcc tcctcaaagg ctccggcggg
     8341 aagggcaagg gaaatggccc ttgacgaagg gggccaaatc atctaacgtt ccatcccttg
     8401 agaaacgccg tgggcagtag aattcaagcc atcgcaaagg gaaagcatcc ggaatggacg
     8461 atctcaacct ggtcatccgc gaacgaatcc agaaggctca agagatggaa gccgcgggga
     8521 tccctctttt ccccaacggg tacgcggtcc cccaccggat cggggaggtt ctggccgcct
     8581 acgaggacca cagcggcgag gccctcgaag cggaagcccc caccttcacg atcgggggcc
     8641 gcatcctggc cgttcggtcc ttcggcaagt ccgtcttcat gcacgtccgg gattcggggg
     8701 gaaagatcca ggtctatctt cagcagaata ggctggggaa agaagcttac gcccgggtca
     8761 aagagctcga catcggcgac atcatacggg ttcacgggac cctatttcgc acccgaacca
     8821 acgagctgac cctcctggcc gccgagttcg agctcctgtc caagagcttc cggcctcttc
     8881 ccgaaaagta ccacggcctc aaagacatcg aaatccgcta ccggcagcgg tacgtggacc
     8941 tcatcgtcaa cgaggaagtc cgccagacct ttttcaagcg cgccaggatc gtccagatca
     9001 tccgcgggtt cctaaacgag cgcaactttc tcgaagtcga aacccccatg atgcagccca
     9061 ttcccggcgg ggccacggcc aagcctttca agacctttca caatgccctg ggaatggaac
     9121 tttacctgag aattgctccc gagctttatc tcaagcgcct cctggtgggc ggcctggacc
     9181 gggtctacga gctcaaccgc aacttccgca acgaggggat ctccacccag cacaacccgg
     9241 aattcaccat gctcgagttc tacatggcct acgccaccta tgaagatctg atggatctca
     9301 ccgaggagct gttcagcgcc gtggcggcag gggtcaacgg gggttccctg cagatcgaat
     9361 accagggcga tgtcgtcgac ctgactcctc cctggccccg gtaccggctc ctggacgccc
     9421 tgacgcagat cggcggtctg cccgcctcgg ccctgggcga tcccgggcag accaaggagc
     9481 ttgcccggga gatggggatt tccccggaac agcacgaagg gcacggaaag ctgctcacca
     9541 agctgtttga cctgaccgtg gagcccaagc tcgtgcagcc cacgttcatc acccattacc
     9601 ccatcgaggt ctccccgttg tcccgccgaa acgagcagga cccgtcggtg acggaccgct
     9661 tcgagctgtt catcgcgggc cgggaaatgg ccaatgcctt ttcggaactc aacgaccccc
     9721 gggatcaaag gcagcgtttt ctgcagcaga tcgaagcgcg ccgggcgggg gacgaggagg
     9781 cccatctcat ggacgaagac tacatccggg ccctggaata cgggatgccg cctgcggcag
     9841 gggagggcat cgggatcgat cgactggtga tgctttttac cgattctccg tccatccggg
     9901 atgtgattct tttcccccat ttgagggcgg agaagaagcc ttaggcggtt ttcacgggac
     9961 gtgacccgga aggagaaacc ctccccggca ggttcacggt tccccccttt ccagcaacca
    10021 agagtcttca tgcccatgac gtttgaattc tttgtcagcc tgcgctacct gttggcaaag
    10081 cgccgtcaga ctttcatctc cctgatcacc atcatctcca tcgcaggcgt ggccgtgggg
    10141 gtcaccgccc tcatcgtggt gctggccgtc atgaacgggt ttcaggaaga cctccggggc
    10201 cggatcctgg gcatcacgtc ccacgtggtg gtgggcaact tcaacggaac catccccaac
    10261 tacgaaacca ttgtgcttga agtgaagaac gcccgggggg tgttggccgc cactcccttc
    10321 acctactccc aggtcatgat cagctcgggg aagaacgtct ccggggccat ccttcggggg
    10381 attgatccca aaacggcccc ggaagtcatc cagctcaaaa agaacctcat ccggggaaac
    10441 ctcgaggacc tcgccccgtc ggatgtctcg accctcgggc ggaacatcat cctgggggtc
    10501 gaactggcca agaacctggg agttcagcag ggagaatggg tcaccctcat tgccccgacg
    10561 ggacgactga ctcccatggg acagaacccc aagagcagcc tgtttcacgt ggtgggaatc
    10621 tttcaatcgg ggatgtacga attcgacaac actctggcct acatcgacct gtcggcggcc
    10681 cagcaattcc tgaacatcgg ggacacggtc acgggcatcg aggtgcgggt caaagacatc
    10741 tacgatgcgg gtgccgtggc tgaatccctt cgttcccgct tgggctaccc gttctgggtc
    10801 cgggactgga tgcagatgaa ccggaacctc ttttccgccc tcaagctgga aaaggtggtc
    10861 atgttcatca tcctcaccct catcgtcctg gtggcggcct tcaacatcgt gagctccctc
    10921 atcatgatgg tcatggaaaa gacccgggac attgccatca tgaaggccat gggagccacc
    10981 acggcccgca ttcgaaagat tttcatcctc gaaggctgca tgatcggcgt atcggggacc
    11041 ctcttgggac tcatgggcgg gttcggcctc tgcgccgtcc tcaagcgcta tcgattcatc
    11101 gaactgccgc ccgacgtcta ctacctttcg actctgcccg tggacctcaa ggccctggac
    11161 gtttttgcca tcgccttgtc ggccatcctc atcagtcttc ttgccaccat ctacccgtct
    11221 cgccaggccg ccaaactcga ccccgcggaa gcccttcgct atgaatagcc atccgaacgg
    11281 acagcccatc gaagtccatg ccatgagcct atccaagatc ttcggaagcg gggtccagtc
    11341 catcgagctg ttccgggact tggacctgat catccgcaaa ggggagcgca tcgccatcgt
    11401 gggtgcctca ggggcgggaa agaccactct cctgcacatc ctgggtaccc tggaaaaacc
    11461 ttccaccggc aaggtcctct acggaggcaa cgacattttc ctttgggacg aaaaggacct
    11521 ggcccagttt cgcaaccgaa acatcgggtt cgtgttccag tttcactacc tgctgcccga
    11581 attcaacgcc ctggaaaacg tgatgatgcc gggcctcatc gccgggcttg cccgaaagga
    11641 agtgcgcacc caggcggagg agatcctcga acgcctggga ctctcggccc gcctcgaaca
    11701 ccgggtgggg gagctctcgg gaggcgagca gcaacgggtg gccgtggccc gggcccttct
    11761 ccttcgaccg aagcttttcc tggcggacga gcccagcgga aacctggaca gccgtaccgg
    11821 ccgaaagctc cacgagcttc tggtttccct caacgaagaa ctggggctca ccatggtcat
    11881 cgtgacccac aatccggagc tcgcctccat gatgcatcgg accctgcggc tggtggacgg
    11941 gcagctgcgg ccccaaccgt cttttgggtt gtcatgcatg gcaccttcgc ctataaatag
    12001 ccagcgagac ggagaatgag aaccacggtg aagactgaga agcttcccgg ggagaagctt
    12061 cgcgagaccc ccggcgccgt cggtcggttc cagggacgat ggtttccgac cgatcacaaa
    12121 cgatgatcag gcaaatgccc atctgagacg gcagcatcgc aggaagaggc ataggaggca
    12181 aaggagagag gaatggcgag ggggacccaa ttcatgagga aattgtgtat cctgttgttt
    12241 ttactgacat tcatcgctcc agtgggatgg gcccagggac cttcggccag ggtgggcgtc
    12301 ttgcccttcg tcatgcatag ccgggagagc ctccccgatg ctcagaggca ggtccaggaa
    12361 ctcttgatcc gccagctttc ggaagaaggt gtgcgagccg ttcccgtcgg ggaggtggac
    12421 aaggtcgtca agccacggga tgcggtccag accgtggagc agggacgatc ctacggccat
    12481 aggcttcagg ccgattacgt ggtcatcggc agcatcaacg tcatcggcgg cagcgtcagt
    12541 ctcgatgcca agctggtgga cgtttccggc cgcaagaaga ccgagcccct ctttgccgag
    12601 gaaaggggcc tcgagaacct tgcttccacc accaagtccc tcatccagca aatgaccgtg
    12661 cacgttctgg ccaaggccgt cattgccgat gtccaggtcc gaggaaacga ccgcatcgaa
    12721 gcggaggcca tcaaggccaa cgtgaaaagc aagaaaggag aggtcctcaa ccgggaacag
    12781 gtgagtgaag acatcaaggc cgtctacaag atggggtact tcgaaaaggt cgaggtagaa
    12841 atggccgacg gccccggcgg gaaaatcctc accttcgtgg tcgaggaaaa ccccaccatc
    12901 gccgaggtaa agccccgggg aaacaagaag atcaaggaaa aggacatcat ggcggccatc
    12961 accaccaagt cgtacatggt ccttcaaaag aacgttgtcg ccgaggacgt gcaaaagatc
    13021 atcaagctct accaccagaa gggatacttc gacgtggatg tgcaaagcaa ggtcgagttt
    13081 ccaaaggatc cccggcgggc aacggtcatc ttcgaaatca aggagcaaaa gaaggtcctc
    13141 atccagaaga tcggttttcg cggaaacaag aaatactccg cccgcaggtt gcgctcggtc
    13201 atgcagacca aggaaaagaa ctttctcctc tccctgttta ccgaccgggg aatcctgcaa
    13261 aaggacatgc tcgccaccga cgtggatcgc ctgacggtgt tttaccacga ccagggttac
    13321 atggatgcca aggtgggaaa tcccgacgtc acccagaaat cggacggctt ctacatcgag
    13381 attcccatcg acgaagggca gcgctacaag gtcgccgacg tgaagatcac cggcgacctc
    13441 ctggaaacct ccgccgacaa gaagacggcg aaagagctca agaccagggc caaggattat
    13501 ttcagccggg agaaggtgcg taaggacatc gacaacattt ccaaggccta catggatgag
    13561 gggtacgccc tcgcccaggt aaaccccgaa gtgaagcggg accccgcctc ccacaccgcc
    13621 gaggtggtct acaacgtctc gaagaaggac aaggtccaca tcggcaagat ctatgtcacg
    13681 gggaacacca aaacccggga caaggtcatc cgccgccagc tcaaactggc cgaaggggac
    13741 ctgttcaacg ccaccaacct ggaaaagagc ctcctgaacc tcaagaagct ggactatttc
    13801 gaagaagtgg aaatcacccc cgtcgagacg gcccaggggg acatcatgga ccttcacgtc
    13861 aaggtaaagg agaagctcac cgggaccatc agcgtgggcg gcggctacgc ctcccaggac
    13921 gggctcttca ccagcgccca gattcagcag agaaacctct tcggaagagg acagaccatc
    13981 gggattcgag gatacctgag cgaggaagcc cagcgatacg tcctcagttt taccgagccc
    14041 tacctgttcg acatgcccct ggctgcggga ttcgacatct tcaactggat tcgcgactac
    14101 gacacgttta cccaggattc ttacggaatt cgtctgagga ccggttatcc gttcgggagt
    14161 tggagccgga tgagcctctg gtacaccatc gaagaggacg aggtcaccga catcagcagg
    14221 aacgccagca agtacatcaa ggaccagaga ggaaaatacc tcaagagcgc cttcaccctc
    14281 gggttcgaac gggacaccac ggaccatccc ttccttccca cccggggaag tttgaacacg
    14341 ctcatgtcgg agttttccag cagctacatc gggagcgaca gcgactacgt gcgcaccgaa
    14401 atccagtcgg gctggtatca tcccctgttc tggaaattca tcgggtttgc aaaggcccag
    14461 tacgggtaca tcttccccct ggatgaccga agcaaggcgg tccctattta tgaacggttc
    14521 ttcctgggcg ggatcaacaa cctgagaggt tggaaatggg cggatatcgg tccccgggat
    14581 tcgcacggcg acgtcgtcgg gggctaccat tacatggtcc tcacgggaga aattctgttc
    14641 ccgctggtcg acaaatacgg aatacgcggc gtcacgtttt ttgacgccgg aaattcctac
    14701 cgggagaaag aggacatcga tttcagctct tttaggacgg atgcaggagt ggggcttcgg
    14761 tggaattccc ccatgggacc tctgcggatc gaatgggcgt acaacctgga tccgaaaccg
    14821 aaggaagaca actatcaatg gcagttttca gcaggagctt tcttttaagg agtggaacac
    14881 atgaagggaa aatggtcgag aagattggtt ctttggacac tgatgggctt caccttgaac
    14941 ttgatggcgg tttccgcctg ggctgcgacg ggcaaagtcg gctacttcga cctccaggtc
    15001 atcctggacc agtcccaggc cggcaaacag gccaagcagg agttcacggc cgaaaaggac
    15061 cgcatgaagg ccaccatgga tcagaaggcg aacgccttca agcaggccaa ggaggaatac
    15121 gaccgcaaga agggcgtcat ggacgagtcg gccaagaaga agaaggacaa ggaactggcc
    15181 gatctccagc aggaagccga aagggccctg atggaagcca ataccaaact caacaagctc
    15241 tcctccgacc tcatggcacc catcgtggac aggatcatcg aaatcgccaa gaagatcggc
    15301 aaagacgata agtacgacta catcctggag gcggggaagg gcggcatcgt ctatgccaac
    15361 gaaaaggaag acctgtccaa gaagatcatc gaggcccttg acaagtcgcc cattcccgcc
    15421 aggaaaccct agtcccgagg ttttcgactc tctgggttcg ccgacctgaa accccggaat
    15481 gaagcgtcct cagtcgtccg gaagcgttcc ttgcgccctc cggtgccggg tcgggcgggg
    15541 gaacgcttcc cagggtttgg cggcccggcc tcccttaagc ttcttcgtcc gttcttccgg
    15601 gtgagggatc ccgcagccat tccaacgaac gtcggctgtc ggcgagagga ttgccgcggg
    15661 acgtcctggg ccgcggaaat gccgcagaca gggcgggccc gctgtgccgg tttcagtgac
    15721 acggagtccc gaagctcttc cactcggtct tcagaagggg ccgaggatgt ggagcggcta
    15781 accgggggtt ttcaccaacc tttacgggga atggaatcat gcaaggtgca caagatccta
    15841 caaggcaaaa gtggttctcc ctgggcgaac tgtcccgcgt tttgggcgcg acccttcggg
    15901 gagatgcgga gatcagggtc cgaggcctcc gacccctgga agttgccgga cccgacgaga
    15961 tcagcttcct gacccacgcc aaatacgtct ccttgctttc ccggtgcaag gccggggcac
    16021 tcatcgtttc gagctcgttt caggatctcg agtacccgct cctcatcagc gataacccgt
    16081 acctgaccat ggcccgggcc gcgcagcttt ttgctcccgt tcccgaggcg actccgggcg
    16141 ttcatcccac ggcttgggtg gcccccgagg cccaggtgga tcccacggcc tccctgggtc
    16201 ccctgtgcca ggtgggaacc gggtcccggg tgggagccgg gaccattctc cacgggggtg
    16261 tcgtcgtcgg caacgaggtg gaaatcggaa acggctgcct cctctacccg aacgtggcgg
    16321 tcctggaccg gtgcaaaatc ggccacaacg tgatcatcca cgccgggacc gtcatcggaa
    16381 gcgatggttt cgggttcgcc caggacgaac aggggcgcca tgtgaagatc ccccagtcgg
    16441 gcatcgtgca aatcgacgac gacgtggaaa tcggttccaa ctgcagcatc gaccgcgcca
    16501 ccttcggccg gacctggatc cagcggggag ccaagatcga caacctggtg atgatcgccc
    16561 ataacgtggt ggtgggcgag gattcgatcc tggtcgccca ggtggggatt tccgggagca
    16621 ctcgcatcgg taaacacgtc gtcctggcag ggcaggtcgg agtggccggg cacttggaaa
    16681 tcggggacgg agcccgggtg ggcgccaagt ccggagtccc tcattccatc aagcccgggg
    16741 aagacatgct ggggattccc gcggtcccgt ccaagcagtt ctttcaaacc ttcgcgaacc
    16801 tccagcgact ttctcagttc aaggaacagc tccggcagct ccagaaccgc gtccggcagc
    16861 tcgaggaaac catgcccgga gaatagacga tggaacaaga catcctgcaa atcctcaagg
    16921 tcctgcccca ccggtacccg tttctgctgc tggaccgtgt cctcgagatg tcttcgcaac
    16981 atctcgtggg gctcaagaac gtgaccatca acgagcctca tttccagggg cactttcccg
    17041 cagaacccat catgccgggg gtgttgatcg tcgaggccct ggcccaggcg ggaggagtcc
    17101 tggccttttc cctggtgccc cagcacatgg ggaatgcggt gtatttcatg ggaatggaca
    17161 aggtgcggtt tcgcaagccc gttcgcccgg gagatcaatt gatcctcaag ttgaacgtcc
    17221 tgaggcagcg gggatccgtg ttcaagatga agggggaagc ttttgtggac gatcagctgg
    17281 tagcagaagc cgagctcctg gccaccatcg accccggcaa gtccgaagag tgaggctcgt
    17341 gacggtgttc ggcatgacgg ggtgaagcgg ggacccggca aaaggagatt tggcaattat
    17401 ggagattcat ccgacggcga ttgtatcccc gaaagcggag ctcggggaaa acgtgaaggt
    17461 tcttccctac agcatcatcg gttcccaggt gaccatcggc tccggatcgg tcatcgggcc
    17521 ccacgccatc gtcgacggcc ggaccaccct tgggcgcaac aaccaggtct ttcccttcgc
    17581 cacggtgggc tatccccccc aggacatcag ctacaagggg gaagatacgg aatgcgtcat
    17641 cggggacggc aacatcatcc gggagagcgt caccattcac cgggggaccg ccaagggggg
    17701 cggaatcacc cgcatcggca acggctgctg cgtcatggcc tacgttcacg tggcccatga
    17761 ctgcaacatc ggcaaccacg tgatcctgtc gagcggcatc ggcatggcgg gccacgtcga
    17821 gattcacgac cacgccatcg tggggggaat ggtggccatt caccagtttg tgcgcatcgg
    17881 ctcctttgcc ttcgtggggg gcatgtcggg cctgaggatg gacatccctc cgtacatgct
    17941 ggcccacggg gcccccgcaa agctctacgg cccgaacctg gtggggcttc gccggcacaa
    18001 gttccccatg cagaccgtcc aggcactcaa aagggcctac aagatcctct tccgctcgac
    18061 cttgaccgtc aaagacgccg tggacaagat ccgcgaagag atcgagcccc ttcccgaaat
    18121 cgagaagctt ctccagttta tgcaggaaga ctcccgtcgc ggggtcacca ggtggggatc
    18181 ggatgaggcc gtcgaatgac ccgctcgaac acccgaagga acgaatcggc ctgatcgcag
    18241 gggcggggca gttcccgctc ctttttgctc atgccgccta ccaggcgggc tttcgcgtgg
    18301 tggccgtcgg gttcgacggg gaaacgcacc cgtccctgcc caagtacgtg gacgagttcc
    18361 actggcagaa cctgggccag ttcaaccggt tgatccagac cttcaagaaa gcgggcgtcc
    18421 gccaggcggt cctggccggg agcatcaaca agaccaagct ctacgcgcgc attcgcccgg
    18481 attggcgtgc cgtcaagttc ctaagcaagc ttcgaaacaa gaaagacgat tttcttctcc
    18541 gagccttctc ccgggagctc gaatccgaag ggatccagat cgctccttcc accatcttcc
    18601 tgccgtccct cctggctccc gaaggcattt tcacccgccg cagacccaat cgccaggaag
    18661 aatcggacat tcttttcggc tggcagacgg caaaggccct ggggcacctg gacatcgggc
    18721 agtgcatcgt cgtgcgcaac caggcggtcc tggccgtcga gggcatggac ggcaccgatg
    18781 ccaccatcct gcgcggggga cacctgtgca aggaaggtgc cgtggtcatc aaggtgagca
    18841 agcccagcca ggacctgagg ttcgatgtcc cggcggtggg atacgacacc attgcgacca
    18901 tgaaacgcgt caaggcccgt gtcctagtga tcgaagcggg aaagaccctc atcttcgacc
    18961 gggagaagat gatcgaagcg gccgacgaag ccgggatcac catccaggcg cttcgagacg
    19021 agaaggccct cgaggaggtg gtggacgagg gccgggattc cgttcgggcc tacctgaaca
    19081 ggctggatcg aagccggtcc ccgtccgtga cgggacctcc tcccgtgctg gttcgcgaac
    19141 cccacgagaa ggctttgaag gtggccgtcg tgggggtcgg atacctgggg cgctttcacg
    19201 cccacaagta tgccgggttc cgggacgcca acctggtggc ggtggtggat atcgacccgg
    19261 agaaggcccg gcaggtcgcc caagaggtct cgtccacccc cctggcggac tatcgggagc
    19321 tcctggggca ggtggatgcc gtgagcatcg tgaccccgac ccctcagcat ttctccatcg
    19381 cttcggattt cctggcggcg ggcgtccacg tcctcctcga aaagcccatg acccagtccc
    19441 ttgccgaggc cgacgagctc attcgcctgg cccgagaaaa caattgcttc ctgcagatcg
    19501 gccacctgga acgcttcaac tcggcctttc aagccgtcct gccccgcttg aaccgaccca
    19561 tgttccttga agcccaccgc ctcgctctct tcaacgaacg gggcctggac gtggacgtca
    19621 tcctggacct gatgatccac gacatcgacc tggtgttgca catggttcgg tctcccttga
    19681 agaggatccg ggcgtcggga gtgtccgtcc tcacgtccgt tcccgacatt gccaccgtgc
    19741 gcatggagtt cgaaaacggg gccaccgcca atcttacggc cagccgcatt tccctcaaga
    19801 accagcggaa gatgcggatc ttccaggaaa acacctatct ttccgcggac tatgccgaga
    19861 agagggccta cgtggtctat cgcgaacagg aatgggacga ggcggggttt ccccagttgt
    19921 ccatcgagga actcgagatc caggaaaggg acgccctggg agaggagatc ctctctttcc
    19981 tggattgcgt ccgggccggc aagcctccgt gggtggacgg ggtccaggga cgccgggccc
    20041 tggcggtggc cctggatatt tcccggcaga tcgaagaaca gctggaaaag ggtcggggag
    20101 aggggggatc gtgatccggg tgagagcatg agccgtacga tcttcgtgag cgcaggggaa
    20161 gcttcggggg acctccacgg cagtcgcctg gcggcggccc tgcgggagct cgagcccgac
    20221 atccgcatca cctgcctggg aggtcccttg ctcgagcagg ccggggcccg ggtcctggtc
    20281 cacaaccggg aaattgccgt ggtggggatc ttcgaggtcc tgcggcacct caaggacctc
    20341 tggagggcct ggaggatggt ccgggagcac atcctccggg aacgaccgga tcccgtggtc
    20401 ctcatcgact ttcccgactt caaccttctc ctggnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnngaacga ccggatcccg tggtcctcat cgactttccc gacttcaacc
    20581 ttctcctggc tcgattggcc cggcgccggg gacttcgcgt cttctactac atcagtcccc
    20641 aggtgtgggg ctggagaacg gggcgggttc gcaccatccg tcgactggtg gaccgcatgg
    20701 cggtgattct tcctttcgaa gaagagttct accgccgaca cggtttcaag gtccattacg
    20761 tgggtcaccc gctcctggac gtcctggccc acgctccatc ccgatcccgg gcgcaggaac
    20821 gctaccggac cggcgaggcg gggccggtgg tgggactgct gccgggaagc cgacgcagcg
    20881 aaatccgatc tctgcttccc atcctcctcg aaaccgcggc cctgcttcga caacggtttc
    20941 cccacatcct tttcctgctt cccgtagccc ccggcctcca ccgggaagag atcgaagagc
    21001 gggcctcgga agcctccctg ccccttcgag tcgtttccgg ggatacctac ggagtcattc
    21061 gggcctgcga cctggtcctt gccgcctcgg gcaccgtaac cctcgagacg gcgatcctgg
    21121 gtactcccct catcctcttc tatcgggtat ccaacctcac cttcaacgcg ggaaagcatc
    21181 tcgttcgagt cccctgggcg ggcctgccca acctcattgc gggaaaaatg attgtgcccg
    21241 agttcatcca ggaccgggca aggccggatc tcatcgccgc cgaagccgcg agcttcctgg
    21301 acgacccggc ccggctggag gctcagcgcc gggaactcga ggccattcaa caccagctgg
    21361 gcgaaccggg cgtggcccgt cgcacggcaa ggcttgtgct ggatctccga cgagagattt
    21421 catcgtgatc gagtgtgttc cgcgggaaag agtcccagtg gcactctgga ctggagggag
    21481 aaaatgaacg ggaaggttgc cgccgtgaaa acggttctat ccttcatcct gatcgtcgtg
    21541 gttttggcgt ggggcatcga gaaccggcag gccggggccc aatccttcaa actggggcca
    21601 aggcaggaac ggaaggcatc gagcccccct gcttcggaac ccggaaagaa agcggaatcc
    21661 gagaaatccg agcccgccac ccctgcggcc ccgggagaaa ctcccaagga ggagccgggg
    21721 aaagcttcgg gggaatccgc gccgcccaat acggacatga aacaggccgg ggcggaactc
    21781 cgggaacccc gggaagaggg tcccccgggt atcaagtggg ccgataacaa tcaagagcag
    21841 cgctgtctat ccatgctggc ggagcttcgc aaggaatttc tccaggcgag gttctattcc
    21901 atccaggggg acgcttgttc cacggccgac cacgccaagt cgtttctcaa gatcgtgaac
    21961 gacatccagc cccaatgccc cgagaacttt ctcgagaaga aaggctaccg acccgtgatc
    22021 ctacggaacc tcaactggct ctacaagctg ggaaccgatc gctgtctcca gtaagatcgc
    22081 tcatcgagcg cccgggggga tgaattcccg ggaaagcttc cgccggaact ggaattcctt
    22141 gcccgcgcag tacaggaccg gggccaccag catggtcacc acttcaaagg tgagtccccc
    22201 gaaggaagga acggccatgg gaagcatgat gtccgatccg cggcccgagg aggtcaggac
    22261 cgggagaagg gccaggatgg ttgtcgccgt ggtcatgaga cagggccgga ttcgctttcg
    22321 gcacgcttcg atggtggttt cccggatctc ccggatgctc gaaaaggacc gccccgcaaa
    22381 ccgctcctcg aggtaggtgg ccatgacgat gttgtcgtcg gcggcgatgc cgaacaaggc
    22441 caggaaaccc acccagaccg ccacgctcaa aaagatggga tgaacccgga agacctcccg
    22501 caggttcgtt cccagcagtt cgaaatttag gaaccagggc tgcccgtaca gccacagcag
    22561 gatgaacccg cccgcccaag ccacgggaat gcaggtgaaa atcagcaggg ccgtgcccac
    22621 ggatttgaac tgcaggtaga agatcacgaa gagaacggca aagaccaggg gaaggatgac
    22681 ccggagccta ttctcggatc ggacctggtt ttcatagctg cccgtgaagg aaaagccgac
    22741 tcccttgggg atctgcagct ctgcgctctc gaccttgaaa ttcaggtaat cccgggcgtg
    22801 ctcgacggct tccacctctg cgattcccgg tttcttgtca aacaatacgt agcccaccag
    22861 gtaggtgtcc tcggtcctga tgacctcggg cccccgcacg taccggatgt ccgccagctg
    22921 catgagagga atctggctcc cgttggccga cgggaccagg attttgccga tggattcgaa
    22981 gtcgtcccgc aactctctca ggtagcggac ccgcacggga aacctctccc gtccctccac
    23041 cgtggtcatg attcggtcgc ctccgacggc aacggaaatg accctctgtg cctgctcgag
    23101 gtcgatttcg tactgggcca aagcttcccg gtccattcgg atttcgaggt acggctttcc
    23161 gatgatcttg tccccaatga ccgacttggg ctcgatggaa ggaacttctc gaaggtagcg
    23221 ttcgatttga gcgcaggcgt tctcgatgga cgggaggtcc ggacccttga cccgcactcc
    23281 catggaggcc cgtatgccgc tctggagcat gaccagccgg gcggaaatgg gctggagctt
    23341 gggtgcctgg gtggagccgg gaatgttcgc ggcggccgtg atggcgtccc agaggtcgtc
    23401 gggattccga attcccttcc aggcccggcg accggggttc aaagtcggat ccaaaggggg
    23461 acgccagagc cggaaaggct ttccgtaggg gtccggaagg agcccgttgg cggaatcccg
    23521 caggaaccgt ccccgaacca cgtaaggcat tccgtccggc gcgttcacgg ggtttccctc
    23581 gatgtcccga tgatagtcgt tttcccggga ggagtaccgg aaagtcaggg gcctgccgtc
    23641 ccggtccgac aggtactggg gtcgatactg gatgagcgtt tcgatcatgg atgccggggc
    23701 cggatccagg ggactttcgg cccgtcccag tttccccacc accgactcca cctccgcaat
    23761 ggcccggatt cgcttgtcct gcttctgaat gatgtccagg ccttccccga tggacgcgtg
    23821 aggcatggtg atgggcatgt agaggaaaga cccctcgtcc agggggggca tgaactcctc
    23881 ccccatgccg ggaaacttgt gagcgacata aacaaggggg gccgtctgtt tcaggaacga
    23941 gggaagccac ccaaaaaccg tgggaaagcc cagccagatg gtggccccga ggagcagcat
    24001 ggcggcgggc aacaggagga aggggccttt atggtccagg caccacccga gaatcggccg
    24061 atagtaatgc atgaggagca gaaacgagct cagaaggccg ccgatgagga acgccacgaa
    24121 caggaaattg aggaacagtc ccttctccgg tccgaaggga agccagtccc gggtgaggag
    24181 cagggccacc aaggcggccg ccaggatact gcccgccccc ttgagcctgc tcttccaggc
    24241 ctcgggaatc cagcggagaa acgtcttgta aaggccgacc aggagaacga agcctcccag
    24301 ccatccatgg acgaacgcca tgagaaggat tcccgccggg atcaagatgg cggataaaac
    24361 ccggccggat gccttcttgt cccgttctcg gaagaacagg aggtgagcca tggcgggaat
    24421 gacggtcacg gccaccacgg cggaacccag gagggcaaag gtcttggtcc aggccagggg
    24481 tttgaagagc ttcccttcgg ccccatccag ggcaaagacg ggcagaaagc tgatgatcgt
    24541 ggcggcggcc gccgccaaca ccgctcctcc cacctcgctg gccccccgga agatcacccg
    24601 gatgcggctt tccccgggat ccgccgcctg cagatgtttc aggatgttct cgcagatcac
    24661 gatgcccatg tcgtccaggg tgccgatggc gatggcgatt cccgccaggg acacaatgtt
    24721 ggcgtccact ccgaaaagct tcatgcccag gaagctgagg agcaccgcca ggggcaggac
    24781 cgacgagatg agaaggctgc tttccaggtg caggatcacc aggaggatga ccacaatggc
    24841 cgtgagggtt tcttgaacga cggcgtcgct cagggttccc agggtctcgt agatgaggcc
    24901 gcttcgatcg tagaaaggca cgatctcgac ccggctcacc gtcccgtcgg cgaggacctt
    24961 tgagggaagt cccgccgaga tctcccggat cttctcctta acgttgttca aagtctcgag
    25021 gggattggcc ccccggcggg ccgtcacgac cccgcccacg gcctcggcgc ccccgttgtc
    25081 cagggcgccg cccctttggg cgggcccgta atgaacgtag gccacgttgc ggatgaatac
    25141 cggaacgtcg tcggtcacct tgatgagggc gttttccagg tccgtcggcc ccttcacgaa
    25201 acccacgccc cggacgatgt actcgacctt gttgatctcg atgccggcgg ctcccacgtc
    25261 gagattggaa cggttggccg ccgccaccac gtcttccaac ttgacgtcga agattcgcat
    25321 gaggttgggg tccacgtcga tctggtattc gcggacatag ccgcccacgg aggccacttc
    25381 cgagacccct tccgcggaga gcagcccgaa gcgaacgtac cagtcctgga gggtcctcag
    25441 ctcgtccagg tcccatcccc ccgcgggctt tccggacgga ccccggccct cgagggtgta
    25501 ccacatcacc tggcccaggg ctgtggcatc gggccccagg gtcggctgca ctcccttggg
    25561 cagggtgttc ggaggaaggc tgctcaactt ctccaggagc cgggagcggg accagtagaa
    25621 ctcgatgtct tccttaaaga tcacgtaaac gttggaaaac ccgaagtagg aatatccccg
    25681 cacggccttg actcccggga gccccagcag ggaggcggtc aggggatagg tcacctggtc
    25741 ctggatgtcc cggggagagc ggccgggcca ctcggtatag acgatctgct gattttcgct
    25801 gatgtcggga atggcgtcca cggggacggg gtaccgggga atgccccgaa gcttccagtc
    25861 gaagggagcg accacgatcc cgcccacgat cacgaacagc gtgaatagga gaacgaggat
    25921 cttcctttcg aggcaaaacc agatgactcg atggatccag gaggagcaag cagggggttg
    25981 ggtcggggga cctcggtcat ccatgggacg acctcaacag gacggaagcg ggcatgacac
    26041 taccagggac tgcggggagc gggagtgggt tgagtcgtat ctccataggt ccccggcctt
    26101 cttcgctgga ttttggcgcg ggagggatgc ctgccttccc catggcccac atcgcttcct
    26161 tccccggcgg ctttcaggtc ctggagggaa gtctcggatt cggccccggt ccgcaggcgg
    26221 ctcttggttc tctgggacca gtattcgtcg tgcagggcct gggaaccgcc cgaaggctgg
    26281 tagttcaagg ccgtatggcc cccctcgagg ctcatcatgc tcgttcgggc caggatctgt
    26341 acggcgctgt cgaccttgaa actcccgttg accaccacct gttcgccctc ctcgaggccg
    26401 tcaaggacca cgtaatggtc ccgggtctta gggcccagca ggatctctcg gccctcgaag
    26461 gcgcccggct ccccgggaac cgccacgtag gccaccgccc gctttcccgt gatgagggga
    26521 gcggatgcgg gaatgaccag gggagcgttt tccggccgat ggggctcgtc cccggccact
    26581 cgaccttccg ccgtcacctc ggcgtgaacg atggaacgaa ccagcatgcc ggacttgagg
    26641 cgtccccccg tgtcgggcag gatcaccccg atttcgaagg ttcgggtctt ggtcttgaac
    26701 gcggggttga tattgacgat atccccctcg aaggtctctc ccgggtaggc atccacctgg
    26761 aagctcacct tctgccccag tcgaatccag ggaaaatcca cttcgtaggc atccagcctg
    26821 gcccaaacat acttgggatc cgccagggtg aagaggctgg ttcccgtgtt gacgtagctt
    26881 ccctcggtgg cgttcacctc gaccacctgg ccgtacatgg gagcgtaaac cgtggcgatc
    26941 ccgttgggct ccccgacctt catcagttca tcgatatccc tcttgggcat gcccaggatg
    27001 gtgagcttgt ggcgcacggc ctcgacggtc tgcatggcct tctcgacctc gggggagcgg
    27061 tgctgagggt cctccttgcc ctttcgctcc tggaccatgg cgtcccgggc aatgtagggc
    27121 tgaccttcct gccacgccag aaagctgggg acgaacctca tggcttccac cagctgctgc
    27181 tgggtgtagt acaggtccga ggaataaatg gagaaaagag gagctcccca ccggacaaac
    27241 tggcccggcc tcttcacgta gacccgatcg atgactccgg gcataaaggc ggtgacgtaa
    27301 ttgacatggg ccgggtcgta ttcgatcttt ccgtagaggc gaatccgggc cgacacgtgt
    27361 tttcgctcga cgggcgctgt ctgcagttcc gccagggcaa cggcttcggg ggaaagcttc
    27421 agggaggttc cgctgcccgt cgcttccatg gaagatgcag gcaccagttc catgccgcag
    27481 acggggcaga ggcccgaggc ttcccggggc gggatgcaca tcatgggaca gagaaaactc
    27541 cgaaccccgt tcttctctcc gggatgaacg tggggcttgc ctgcaaggcg ctgtgccggc
    27601 ggcgccgtct cgagcggatc cccgtgggtc agggacccca gggaacgaat tccgaatccc
    27661 accacgaagg acaacaccac ggccacggca accttatagc gcggtgggcc tgaaaaaaac
    27721 gtctcgaaca atttcttcat agttgacaat cccgcctaaa ccaagcggtt cctgccgacg
    27781 ccgaggccct gcgctcattc gaccttaagg ttttccacct tggcgtcccc ggacaccagg
    27841 gtcacaaggt tcccgctcac gaccgttttg ttgagatttc gaaagagaat gttgtacacc
    27901 ttgtcggtcc ggggcttggt ggaagtggag cgcatgctgc ccagtcgtgt catggatacg
    27961 ggcaacttct tccccgtggc ctggtcgatg agggagactt cggtcttgcg ggccatgaag
    28021 ggcttggcct tctccgcatc cacaacccgg tagcgacaat ccacgaagtg gtcggaggcc
    28081 gtcatgcgca gggcaacgat ctgaatgccc aggtcctttt cggtggcggc actggcggga
    28141 gcgacaaggg tcaggcagaa gacgaagcaa taaaacactg ccgccaaaac cggcaaagcc
    28201 gggattcctt tcatgacgcg tagattcaaa ggggcatgag tctgcatggg tacctctctt
    28261 tcctcttgtt ggcgctatga accggggggc cggaaactct tgctcccccg aatcgaaggg
    28321 aacgattctc acccgctggg ggcgggcagg gcatgttcct ttttccctcc ccagtccatg
    28381 gaggcggaga aggacggacg gcgaaggtcg gtcaggcttg cggtccgcgt tgcgccttct
    28441 acattccaca aaaataggcg tggttcaatg gggtgcagac ggtatttatc gggtggtggg
    28501 acggtagagg ggggtggtgt cgcctttccc gacggttcca acctgggggc ggattctcga
    28561 cgcggccatc gaggcccagg gctggcggga aaagattgcg agaatcgagc agggaagatc
    28621 gtcaggctga attgcagagg ctcgaacgga cggtttccta aacacggcca acccgcccgt
    28681 gtacccttcc cgtttcattc ccggatctcc tacgctcctg ctttcttgga aacccttcgc
    28741 ccgggtcgga aaacgatcgg gatggaaacg gacgacacgg gcaggagatt ccggtccgtg
    28801 tcggcggcac ggcaagatgg ggcgggcaag ggcccccact actgcgagaa gctcctgccc
    28861 gtgtcgatcg gtaatggttt gcccctaggg caccgccacc gtggcggatc ctccggcgct
    28921 gctggtgagc gtaacgctgt cgggcttggc ggcaaccccg ctgaaaacct cacggtactc
    28981 gccgaaattg gccctgtaaa tgagctgacc caggtcggcc ggggtgcccc ccgtgctggt
    29041 ggaagccgag agctccgccg tgccgccggg ggcgttggac gtcgcccaaa cgccaaggct
    29101 ttgtcccgcg gcgttgtata ccgccttggt gatggtcacg gtgtccgtca ccccgagaaa
    29161 ggcgatcatt ccccccgggg ttggggcccc tgcattggtc acgtcgagcc gggcgtccat
    29221 gatgggaatc cttcccgccg tggcggtaaa gatggcgtca atggtctttc ccgccgggag
    29281 ctcgaaggcg tactggcttc gaggaaagtc gtagggattt ccgtcctcgg ccacggcatt
    29341 caagtaagtt cccaggaact ggggaatgtg ggtgtcgagg catgcgctca caaaccggaa
    29401 caggatcttt tgtcccgggg tgatgggatt gatggacgcg ttgatggggt tcagggacgc
    29461 gtccgggtac gatttcccgt tgatgaggga atacttggga gcccggtggg aactgctggg
    29521 aatggttttc ccgtacccgt agtttccatc ggcgacggcc cgctggatga ccgggtcgat
    29581 ttcgtggaaa agcaccacca gctgtttgtc gtaggcgacc cccgtgtagg cctggccggg
    29641 agccgcatct ttcaccatgg ggagcacgag gcccatctgg acctgcttgg ccgggttggt
    29701 gccgctcgag agaaggtagg tccccggtcg gaagttatcc caccggtagc tgccgacccc
    29761 gcccgatccc gccgtctcgt ggctgaagga tcgcacccgg gccgtgtagt tctccgccgg
    29821 tctcgatccg gtgtaggtca cggtcgtgtg tccctcggtc caaacggggc cggagttgtt
    29881 ggagagcacc tggccgagga tgtgcaagga ggtctcctcg ggaagctggt taatgaggct
    29941 taccgtcacc cggttctggg cggggggcac caccagggtg gggcccggga ccttcaccac
    30001 gtcgtctccc ggggtcgttg tgccgtcttc gttcgtggac accagggcat atccccaggc
    30061 cgggaccact actccgtcgg ccatggtgag gttcaccggg gccgccctca ggttgaaggt
    30121 gatgtccgcg tgggctgccg gggccgaaac ggtcaggaga aacagggcca agatccagcc
    30181 ccgcactcga ttgttgccgg tttttcggta catcatggtt tcctcccttc gtcgtcaagg
    30241 acggattcta aggaatcgga acgttcgggt gttcaatgat catgaaactc accaaacctc
    30301 ccggccagat gtcgttgctg gtaagctcct tttccgtgtg ggaatgccac atgtagaaat
    30361 acccggcatt ggcattcagg cccggatctc ccggaggaag gtctccgccc gctcccagaa
    30421 agggactgcc gctgtagaaa ggaccgtaag tcatggaatc ccgggtgggc agaaccaccg
    30481 gaaagggctt gccgtggtcc ggagcgtatt cacccggctc catggggtcc cccggggcat
    30541 gaccgtagat gtcccaaccg agcttttcgg cgttccagct ccagatggcg tcggcggtcc
    30601 ttccggcggc gatcttgtaa gtcacgtcgc tccaggccag gtccgctcca ctcaccccgg
    30661 gattgctcga aaacatcctt ccgtcctcgg caatgaccca gaagttgttc ccgtgaaggt
    30721 gcatgggatg aatgtctcga ccccctccca cgagccgcac caggattttt tgtcccgggt
    30781 gcattctcag cacgcagttg taaggctggg tggggagcca cttggcgaag gccgggctca
    30841 tgacgtcggg ccagtttctg ccgttgatga accaattggt gggaaaatac ttggtggtgt
    30901 ccacggccga tgcgggagtg aactccacct ggcggtgaat ccagggatcc atttccgtga
    30961 tgaagtaaag gtattcctgg tcgaagtacg aatccgcgtg gttgtaggca tggtgggcgg
    31021 ggtccgggcc gccgccgggc cgcacgatca ag
//
LOCUS       DAOOVC010000034        30935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_994_length_30935_cov_7.743361, whole genome shotgun sequence.
ACCESSION   DAOOVC010000034 DAOOVC010000000
VERSION     DAOOVC010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 30935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30935
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_994_length_30935_cov_7.743361"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLM79_06635"
     CDS             complement(<1..549)
                     /locus_tag="PLM79_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86021.1"
                     /translation="MSANAEKVTDRKDPEVRIGVFVCHCGSNIAGYLNCEELTAYAGT
                     LPGVVYTKNNLYTCSEAGIAEIQQAIKDKDLTRVVVASCSPRTHMPLFKSACAQAGLN
                     AYLFEMVNIRDQCSWVHMQEREEAMAKARDLIRMGVAKAAFLEPQQDIESSLVRRAMV
                     IGGGVAGLSSAEALAGMGVEVVL"
     gene            complement(564..2096)
                     /locus_tag="PLM79_06640"
     CDS             complement(564..2096)
                     /locus_tag="PLM79_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271121193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1929..1931),aa:Sec)
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV86022.1"
                     /translation="MNRDVLVIGGGIAGIQASLDLAEMGFDVHLVEKLPSIGGKMAQL
                     DKTFPTNDCALUILAPKLMDVGRHPRIHLLAYSELEKLEGSVGNFTATVRKKARYVNE
                     SKCTGCGGCSEKCPTVVSDEYNLGLGKTKAIYRYFAQGIPSTYTIDARYCRQLQGKKC
                     GVCAKVCQAGAIDYEQKDQVLEVPVGAVVVATGYELFDARRISEYGFGRFPNVVSALQ
                     FERFLSASGPTHGHVYRPSDLALMASLEELEKNHSKAQRGLENYEKKFKQSSDAFYPL
                     YESGELRGDDYDKWAAQVKAFRALTRELEETRRRVGAVHGAHRLAFIQCVGSRDIRFN
                     PYCSGFCCMHSIKEAIIAREHDPEAEVFIFGMDIRAVGKGFEEYRNRGATEAKIQYVR
                     SRVAEITEDGDHAPVLWYEDTAERRVKSLTVDMVILATACEPSPSVRSLSGLLGIAVN
                     DFGFFQTDPAQPLDSSREGIFVCGCAQGPMDIPESVAQSSSAAARAAQVLSDLSSVHL
                     AS"
     gene            3002..3934
                     /locus_tag="PLM79_06645"
     CDS             3002..3934
                     /locus_tag="PLM79_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86023.1"
                     /translation="MGSDRKLVLLALDGSDQSLEMVRYISRVLTLSNTEVVLYHVFQK
                     VLPIFWDREQDPVAAKHLEYMEGWSAHKEEKVREFMMQARDLLVSAGIPEESVTISVQ
                     HRKQGIARDILEESRFGYDVVALGRRGLGTTDDTMLGSVASKVLLHVSGPAVCLVGGT
                     PKPGKVLVGLDNSWGAMRAANFVAKLLNKGEPQITLAHVVRFPAMEEGEGLNGSYIEK
                     LQQDAEDAIKPVFEKVTKSLLGLGVPASKIGTKVITGVVSRSTALLNEARNGRYGTIV
                     IGRRGVSDVEEFEMGRVAAKLTQVARDMAVWVVS"
     gene            4305..5294
                     /locus_tag="PLM79_06650"
     CDS             4305..5294
                     /locus_tag="PLM79_06650"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOV86024.1"
                     /translation="MKMSSKRRRGAVLVLAVLLLGLGIAGAADKKAVPPSGEEGSAGS
                     LTLWKALVLGAVEGLTEYLPVSSTGHLLMAERLMGMYGRDGEPLSRRRQAKTAADAYA
                     ICIQGGAILAVLGLYARRVKQMILGLAGKDPSGLRLTLNLAAGFLPAAVIGLLAGDLV
                     KQYLFGAWPIVAAWLAGGLAILVIARWQRRSMGTRKEKDLTQLNWRMALMIGLVQCIA
                     MWPGVSRSLSTIVGGLLAGLSLPAAVEFSFLLGVVTLGAATSHDALKHGGDMLQLFDA
                     TSIALGLISAFVFAVISIRWMVSYLTRHDLSLFGYYRIALAAFAGFLILTGRL"
     gene            complement(5537..6886)
                     /locus_tag="PLM79_06655"
     CDS             complement(5537..6886)
                     /locus_tag="PLM79_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOV86025.1"
                     /translation="MSKTIHIAPITRIEGHGSIAIHLDDAGNVKDTKVHYMSLRGFEK
                     FIEGKPAEEVPRIVNRICGICPWHHHLASNKAVDRCFGVTIPPAARKLREFVQTVSHA
                     EDKLLNFYFLAAPDFVMGPDANYEVRNVIGVAKANPELAAKAVRMRYMVKMILEKVGG
                     KAIHPHAGVVGGFCRALLEEERQEFRKQLSELLDFASFTLEFAKTQVFPNYLDAVQAL
                     GVIRTGFLGTVDEDGNLALYEGKLRLMKADGTFTDFSTQDYADYLGEAVVPYSYGKFP
                     YARCWGEGFSLDIDHPKGIYRANCLARINVADRIATPRAQAELEEFRAAFGRPAQATL
                     LYHWARLIEDVYAYEHALELLEESDICDPNVRTPAEPRAGRGVGCVEAPRGTLIHDYS
                     TDDNGLISRANLIVGTTHNLGPINMSVNQAAKLLIREGKVDEGILNKIEMSVRAYDP"
     gene            complement(6890..7828)
                     /locus_tag="PLM79_06660"
     CDS             complement(6890..7828)
                     /locus_tag="PLM79_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl viologen-reducing hydrogenase"
                     /protein_id="HOV86026.1"
                     /translation="MGVKVAEEWLNACSGCEISVVDLGERLLKVLELVDFVHIPVLMD
                     HKYFGQLGDGKHIEIPEAEVGIISGSIRNEEHLEIAHEMRKKCKIIVGFGTCATHGGI
                     PALANSYTNEEIFQRYYSTETTDRVENLPSEGIPPLLDSVYALDEKIKVDVYLPGCPP
                     HPDQVFNALAALLGIMPLSLADKSVCDQCPTIREGKGQVKQLRRFLQSPQYNGPDEPL
                     DKMRCLLEQGFLCMGAVTKAGCGGDKITPRCITARVPCRGCYGPVQQDGNQLLAMLNA
                     LASNGIDIKSLPEYASLLRFSGAHGMLRPERKRKEA"
     gene            complement(8123..8548)
                     /locus_tag="PLM79_06665"
     CDS             complement(8123..8548)
                     /locus_tag="PLM79_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8498..8500),aa:Sec)
                     /transl_except=(pos:complement(8345..8347),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV86027.1"
                     /translation="MSDPFKPVILGFLCTWUAYAAADLAGVSRLQYPTNLRIIRVMCS
                     GMVSPDLVVHALSKGADGVLVVGUHLGECHYLEGNQKASARSDVVKVVLDSCGIDSNR
                     FRAQWVSSAEAPKFAEVVNRFIEDIRELGPNPLRERKVA"
     gene            complement(8609..10327)
                     /locus_tag="PLM79_06670"
     CDS             complement(8609..10327)
                     /locus_tag="PLM79_06670"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV86028.1"
                     /translation="MSGNGEMVVNPEAKEARIGVFVCHCGSNIAGLLDCVQLTEYAAG
                     LPGVVYTKNNLYTCSESGIGEIKKAIGEHQLTRVVVASCSPRTHMPLFKSACDEAGLN
                     PYLFEMANIRDQCSWVHMQQGEDAMVKARDLIRMAVAKASLLAPQKDIESSLVRKALV
                     IGGGVTGLSAACTLADMGVEVVLAEREARLGGLLNRINRLAPGGEKASDVVEKLVSRV
                     KGTPRIQTLLGAEVLSVGGVIGNYHVTLRNGEGKEIEETVGCILVATGAVPLKPEGMY
                     GYDGKTVVTQIELEEMLRDGSFNARKVVMIQCAGSRVPEREYCSRICCLTAVKNALYI
                     KEQNPEARVHILYRDIQMYGTENERMLWESRGKGVRYDVYDVNCPPVVKGNTVEVCQP
                     LTGDVLEIPFDLLVLSTPLVARDDAAALANLMRIPVDQNRFFLEAHAKLRPLDFATDG
                     IFLCGTARYPATVGEAQAQGFGAASRAATVLFKDKLISSALISRVTEHCDGCALCVDV
                     CPYFAIKLEEYERDGRKVKRIVSDEILCKGCGVCAATCPKGGVEVNGFTLDQLKAQVD
                     AALEAV"
     gene            complement(10342..11877)
                     /locus_tag="PLM79_06675"
     CDS             complement(10342..11877)
                     /locus_tag="PLM79_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271121193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(11710..11712),aa:Sec)
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV86029.1"
                     /translation="MSRDVLVIGGGIAGIQASLDLAEMGFQVNLVERLPTIGGKMAQL
                     DKTFPTNDCALUILAPKLMDVGRHPKIRLLAYSEVEKVDGELGEFRVTIRKKARYVDE
                     NKCTGCGACAEKCPTPVRDEYNLGLAESKAIYRYFAQGIPSTYTIHAQYCRNFQPGKK
                     CGVCSKICQAGAIDYKQQDQILEVEVGAVILATGYETFDARRIPEYGYGRLPNVITAL
                     ELERFLSAGGPTHGHVYRPSDLALMAALPDLEKARQRARKGLEKFEKTFDRTSEEFFP
                     LYESGELAGEDYDQWAEQVRAARKADGEFEGLRERVDKIETAHRLVFIQCVGSRDIRF
                     NRYCSGFCCMHAIKEAIIAKEHDRQAEVFILGMDIRAVGKGFEEYRNRGGRESGIQYI
                     RSRVAEVTEDADHNLTVWYEDTKERRVKQLAADLVVLSTACQPAASSKRMAELLNIEV
                     NDFGFFKTPGYPPMDTTRPGIYVCGCAHSPMDIPESVAQASSAAARAAQVLSGSESAC
                     LAS"
     gene            complement(11979..13100)
                     /locus_tag="PLM79_06680"
     CDS             complement(11979..13100)
                     /locus_tag="PLM79_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV86030.1"
                     /translation="MILSQQQIDFCMECGVCTGSCPISRVIPTFSPRQMIKRTLMDRD
                     ETVLQSRELWACLTCARCSSRCPVQIDFPDFARIHRELARKEGNLPQLSHHGTLQAIV
                     QLQATGVKQKRTDWARDVGKFQASGDTFYFVGCAPYFDAVIKYGDTSLETAKSVLRLL
                     NRMGVEPVMSDDERCCGHDALWSGNEELFRRLVSMNLEVIRGSGAKRVVFSCPEGYAT
                     YKYHVPKFFGDLPFEVLHTTEFFARELPGSGVAFRPAEDPGNGKVTYQDPCRLGRVAG
                     IYEPPRDLLKMLPQVVFEEMGRNRENALCCGTSAWIECSNCSKTMQIERLEEARDVGA
                     STLVTACPKCRIHLTCAQSNTDLNLNIVDLYSFLLTRME"
     gene            complement(13193..13648)
                     /locus_tag="PLM79_06685"
     CDS             complement(13193..13648)
                     /locus_tag="PLM79_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(13445..13447),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV86031.1"
                     /translation="MSENYEPKIVAFVCNWCAYAGADLAGVARMQYPPNVRLIRLMCS
                     GRMNHGFLLNAFMAGADGVMVSGUHIGDCHYLGGNVKCQKVVEETHKVLNLFGVDERY
                     LRLKWISASEGGIFAEEIRTFTNLLKQLGKNPLTDSAPGQGGSEVGSAA"
     gene            complement(13716..18146)
                     /locus_tag="PLM79_06690"
     CDS             complement(13716..18146)
                     /locus_tag="PLM79_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86032.1"
                     /translation="MAQQKVGAVAVVGGGIAGIQSALDIANSGFKVYLLEEQPNVGGV
                     MAQLDKTFPTNDCSSCMMGPKLVELANHRNIQILTKSEVEGISGEPGNFTLTVKRHPR
                     YVREDRCTGCGECEKVCPVDLPANFNLGLNSRKAIYRNYPQAIPPTYSIDKGDKSPCT
                     NACPNSVNAHGYVALIAQGRYQDAMGVILRNLPLPGVIGRICPHPCETACRRGEVDAP
                     VSICALKRFVADKVDIEQWPVPEVAKREEKVAVIGSGPAGLTAAHFLSLEGFQVTIFE
                     ALSVPGGMLRVGIPDYRLPPEVLEKEVRYITRLGVDIRYNQRLGKDITLDGLEAQGYK
                     AVYLAIGAHSSLKLNIPGEDAEGVMHGVEFLRKANLGQAPDIRGQRVVIIGGGDVAID
                     AARCALRLGAQKVSIFYRRSRQEMPARENEIEDALAEGVEIRYLTAPQQVLTRDGKMI
                     GIQCIRMELGAPDSSGRRRPIPVPGSEFTSEAEMLIPAIGQTPDSLFLAESQGLKLTR
                     WGTIEADEITFATSRPGVFAGGDAQTGPWVAIGAVAAGREAAVSISRYLKGEDLKAGR
                     EPKGKVSQENFRPIPEDAQIQPRAEMRTIPMSKRKAGFGEVELGLTEEQALAEARKCL
                     NCMTCCECLQCVDACMAKAIDHQMEGGYEQIQAGAVIFAPGFKPFDARLKPEYGYGLW
                     PNVVTSLEYERILSAAGPFHGHIQRISDGGKPQRIAWIQCVGSRDKSIGRDYCSYVCC
                     MYATKQAMITKEHEADIETTIFYIDMRAQGKGFDRFYERARDETGVRFVRSMVSRVVP
                     VPESDRLRVCYSDARNELREEEFDMVVLSVGLCSHPSTAELAKRLGVELTSHGFTATD
                     PLDVVASSKAGVFVCGVSQGPKDIPDTVQQGSSAASCATALLSEARGTLITPPPQYEE
                     RDIRGEKPRVGVFVCHCGINIAGVVDVEAVAEYARSLPGVEYATHSMFTCSTDQQKDI
                     KRIIEEYKLNRVIVASCTPRTHEPLFRNTLREGGLNPYLFELANIREHDSWVHQGDPV
                     AATNKAKDLVRMAVARVSLLEPVYDFTYEVVQKALVIGGGLAGLTAALSIAEQGFPAI
                     VVERSAELGGNARTLHYTEDGANPARYVQELIEKVESNPLITVLKNAEVTSVMGSCGN
                     FVTNVAVNGAAREISHGVAIVATGGEEYKPTEYQYGKHPKIVTQKEFEAMLSTEPDKA
                     RGIRKVVMIQCVGSREPENSYCSRVCCTSAVKNSLKMMELNPQAQISILYRDIRTFGF
                     KELAYQEARTRGVRFFRFEEDQKPEVDVSGGSVQVSMMDAQLLRRVRLDADLLVLSAA
                     IRPRAESKKLAEVMRLPLDQDGFFLEAHLKLRPLDFATAGFFLAGLAQGPKFANEAIA
                     QAKGAVSRAVTILSQKEIVAEGVITQVNGELCRACGECEQTCLFEAIKVKEVEGGRRQ
                     ATVNQALCTGCGACNAACPTGAASLAHFRDKQINAVIRAL"
     gene            complement(18232..19089)
                     /locus_tag="PLM79_06695"
     CDS             complement(18232..19089)
                     /locus_tag="PLM79_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOV86033.1"
                     /translation="MMNVTYYPGCSLEGTARDYADSIRGVCASLGITLEEVPDWNCCG
                     ATAAHSIHHRASIELAGRNLKLAARVPNKDMVVPCPLCYNRLKTASHVLGSREAHQYR
                     MALDASTPKIWDLANFLATDDMLGKIKERVKKPLEGLKVVCYYGCQASRPPSVTESTD
                     WENPQSLDKIMKALGAKPILWPYKTDCCGASLMLSRPDMGSRLVGKLFDMARRVGAQA
                     MVVSCQMCQSNLDMYQEKIEKDWGRPIALPVPYFTELIGLALGIEEATSWMSRHFADP
                     RPALRAARI"
     gene            complement(19183..19749)
                     /locus_tag="PLM79_06700"
     CDS             complement(19183..19749)
                     /locus_tag="PLM79_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV86034.1"
                     /translation="MQQTVLNPDFSFLEKAEAAGPFYAAQCFQCRKCTNGCPATFAMD
                     LYPDQVIRLAILGQKDEVLRSKTIWVCASCETCTTRCPNGVRIAELMDYFKELAVRES
                     IPSPEPQVLALHQAFLETVQYAGRVFEGGLVPLYTLRSGQMFSKIREGSWRDDLRMAW
                     RLYKKDRLPLVPKVIEGKEEVRDLLRSS"
     gene            20410..21732
                     /gene="der"
                     /locus_tag="PLM79_06705"
     CDS             20410..21732
                     /gene="der"
                     /locus_tag="PLM79_06705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOV86035.1"
                     /translation="MTVVAIIGRPNVGKSTLFNRIAGRRHALVDDFPGVTRDRNYAPV
                     SWRGKTFTLIDTAGFLDSTESGLEEQAREQILLALDEADILLFVADGNTGLHPEDRSL
                     VRMLRRSSKPSYFAVNKVDGPEKARLLGEFFELGLEKIYPISAAHGYGVADLLADLTR
                     AVPDAPETNAGEDDPGEIRLAVVGRPNVGKSTLVNRLLGAPRVIVSPVPGTTRDAVDS
                     PLRWRNRSYLLIDTAGIRRKGKTREKLEKISVLKALQSIERSHVSLLMLDASEGITDQ
                     DLHIAGYIQDQSRACLVILNKWDLLAADPKRQKRYGEEVRDRMRFMPFAPLLPVSAQT
                     GRNLSKILPTTNEIFEQYNRRITTGVVNRALERTLARHEPPSAGNRRLKFYYATQAST
                     RPPTFVLFCNDPESVHFSYKRYLINQFRESFQLDKTPIRILFRGRKSD"
     gene            complement(21729..22559)
                     /locus_tag="PLM79_06710"
     CDS             complement(21729..22559)
                     /locus_tag="PLM79_06710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV86036.1"
                     /translation="MKVSVVIPTYNRAAFVEEAVRSVLAQRGGVSFEIIVVDDGSTDD
                     TRERLSRLGSGLRTVCQDNRGVASARNAGLALARGEWIAFLDSDDLWLPQKLVKQLEF
                     LADSPGLLLCQTEEIWIRNGRRLNPRKCHAKPEGHCFPRLLERCLISPSAVMIHHRVF
                     EEIGGFDEDFPACEDYEFWLRTGARYPFGLVREPLVVKRGGHSDQLSARIPALDRYRI
                     QALVKLLRETPLTPDQRTLALAELAKRCRIYAHGCRKRGRIEEADGILRLPGQTAEEP
                     "
     gene            complement(22597..23403)
                     /gene="murI"
                     /locus_tag="PLM79_06715"
     CDS             complement(22597..23403)
                     /gene="murI"
                     /locus_tag="PLM79_06715"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOV86037.1"
                     /translation="MQEKPTNPIGVFDSGIGGLTVVRALMERLPFENIVYFGDTARVP
                     YGVKSVETIAHYARQITEFLLQQQVKLLIIACNTMAAVAYQVVDDLSAVKVLDVIDAG
                     AREAAAGTRVKSVAVIGTPATINSNAYARAIHRYDPEIRIFSQACALFVPLVEEGWLD
                     HTVTRLTAEEYLKPVLCHSIDTLVLGCTHYPLLKPLLQEVAGPDIRLVDSAEAMAEQT
                     AAVLQERDLENREGAVPEYRFYVTDVPLRFQSIGERFLGRSLSNVRVVQW"
     gene            complement(23476..25398)
                     /locus_tag="PLM79_06720"
     CDS             complement(23476..25398)
                     /locus_tag="PLM79_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86038.1"
                     /translation="MKKRVLVVDNHPLILKFMSTLLTRRGYDVRVAEDGLSALDILQE
                     FTPQVVFIDLIMPNIDGKKLCQIIRRMPGFRDLFIVILSAVAAEEELDIAKLGANACI
                     AKGPLDEMAANVELALDESERVEAGHPPKSPVLGKIFTPRAITRELLTEKKHFELILE
                     SMAEGILEITGEARIVYLNRVAKDLIARPEEQILGSNFKELFNAEDRERVQGLLDSTN
                     GSTAVISLGAPVKILNKQISINLIPIQEERRAIVLLNDVSERKRMETLLQQTQKMEAV
                     GVLAGGIAHDFNNILMAVLGNISLAKQFAEPRSPLFLKLQEAERASLRAQDLTQQLLT
                     FSRGGAPIKKTLSMASLIRDSATFALRGSNVRCDIAIQEDLWPAEVDEGQMSQVVNNL
                     IINADQAMPDGGTVRVAAGNRILEEKNPQGIPPGRYVFLSIKDEGIGIPEDLLSKVFD
                     PYFTTKQKGSGLGLTMVYSIVKKHDGYVIVDSRLNEGTEVQVYLPASWGRVPECRNFD
                     VKARTGRGRVLLMDDEELVLTVVCGMLEYLGYECEAARDGEEAIRIYESALKAGRPID
                     AVIMDLTVPGRMGGKIAVKKLLEIDPGVKAVVSSGYSNDPVMAHYERYGFCGVVTKPY
                     RIEQLSEVLHRVLHKV"
     gene            complement(25667..25996)
                     /locus_tag="PLM79_06725"
     CDS             complement(25667..25996)
                     /locus_tag="PLM79_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOV86039.1"
                     /translation="MDLKGMAEKLGFEEKDFLELVQIFVDATRTDLDLLEQAVAQGDL
                     KRLIECAHSLKGAAGTLGIRSIQDMAKVLVDNARKNILEGSTEAVAAIRKDVERLAAT
                     IEKKDKL"
     gene            26442..27629
                     /locus_tag="PLM79_06730"
     CDS             26442..27629
                     /locus_tag="PLM79_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86040.1"
                     /translation="MYLSSFIHREELFHIAQRWFCGRMEPDDALKLTQILICDGFVLG
                     QTLQRVTRTLLGRICADCMHEEPIRIKGQLRDALCRSPRVFTPRTRELCREYRANPEY
                     YYAEVPINGALYTDSNHALWGVYRIKRPRRIAEKANRKIAQWIFERVRHRAQEMALER
                     AHRYDIPLDRLVTPENEMVEEFIQAEKVIARSFSDGTMSLDPSVMTIHDVGGIKVVAD
                     REDLDFLEKSLSANSDWKVLDRENFRGDYRAVSLILQVPWEREEVCRQYRETRSWRKY
                     LNRGIGEDELKKGLEPLLENAEAHLNLELILSTFEDLVESELGNSIHEERITAQREAR
                     VYKGYIPMNVEFLLGYLFAVGFSPRVRIDSLPVRLWGRYLPDTLLSHFRRLYSLPEYD
                     LLS"
     gene            complement(28110..29966)
                     /locus_tag="PLM79_06735"
     CDS             complement(28110..29966)
                     /locus_tag="PLM79_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV86041.1"
                     /translation="MKRKTVPVFLAILVLVLISPHLAHAKLKASQPKFPNAKLLVSAQ
                     SVQDSLGEDNLVIIDARTSGYDTSHIPGAINLKFADFFTPGVGLLPKSDLRRKLGEAG
                     LTRTMTFVIYDNTSASFGAAGRIFWMLEYLGCTDVHILDGGWDKWAADGRPEEATINI
                     LPAATFEASVIASRRMTKERISKKLNTKNFVVVDSRTDEEFIGWQLYGEARGGHIPGA
                     VQIPYGWFFNDNKTVRKYETLKEMFESRGITRKKEVTAYCTCGIRSAFVYFLYRLMGY
                     SKASNYDASILEWAADRSLPMEKARNYSKLVYAQWVKDLLDGKPVPNPPAGRYVLAAT
                     TSSGSVDTRRLIPGSIYIRTEEVEWPSAEAGLVEGSGNLWPDKKLKKKIEKMGIDKDT
                     TVILYDAAYGSADPISTTRVWWALLYAGVSDLRILNGGITSWMDSGFDVTEKPEARNA
                     VDDFGTGPGEFPLHPEYLATTDYVEEVVAGKHPKALIVDIRSWREYIGGPNDYASIGI
                     PLTARGRIPGAMWGHWGPKTLVGGHFSDSLDSTLRSYLEVARFWKEWEITRDRTLIFY
                     CGTGWRSTLGLFFGHLMGWPSLKNYDGSFLEWAHTSPNAKNHKIEKGNPLRD"
BASE COUNT         6077 a         9474 c         9027 g         6357 t
ORIGIN      
        1 cagcaccacc tcgaccccca ttcccgccag ggcttcggcg gaagaaagcc ccgccacccc
       61 gccaccaatg accatggctc gccgcaccag ggaagactcg atatcctgct gaggttccag
      121 gaaagcggcc ttggccacgc ccatgcgaat gaggtcccgg gctttggcca tggcttcttc
      181 gcgctcctgc atatggaccc aggaacactg gtcccggata ttcaccattt cgaacagata
      241 ggcgttgagt cctgcctggg cgcacgcgct cttgaaaaga ggcatgtggg ttcgagggga
      301 acaggaggcc accaccaccc gggtcaggtc tttgtccttg atggcctgct ggatttcggc
      361 aatcccggcc tcggaacagg tatacaaatt gttcttggta tacacgaccc ccggcaaggt
      421 cccggcgtac gcggtcagtt cctcgcagtt gaggtacccg gctatattgg agccgcaatg
      481 gcacacaaag accccgattc gaacctccgg gtctttgcga tccgtcactt tttcggcgtt
      541 tgcactcatc aaccaattct cctctaggat gcgagatgga cggacgaaag gtccgaaaga
      601 acctgagccg cccgggcggc ggcactgctg gattgagcca ccgattccgg aatgtccatg
      661 ggtccctggg cacagccgca aacgaaaatc ccttcccggc tcgagtccag gggttgcgca
      721 ggatccgtct ggaagaagcc gaagtcgttc acggcaatcc ccagaagtcc ggacagggac
      781 ctcacggaag ggctgggttc gcacgcggtg gccaggatca ccatatccac ggtgaggctc
      841 ttcacccgac gctccgccgt gtcttcgtac cacaggaccg gcgcgtgatc cccgtcttcg
      901 gtaatctccg ccacacggct ccgaacatac tggatcttcg cctccgtggc tccacggttc
      961 cggtactctt cgaagccttt gcccaccgcg cgaatgtcca tgccgaaaat gaagacctcc
     1021 gcctcgggat cgtgctcccg tgcaatgatc gcttccttga tggagtgcat acagcaaaac
     1081 ccggaacaat aggggttgaa tcgaatgtcc cgggagccca cgcattgaat gaaggcgagt
     1141 cggtgggccc cgtggacggc acccactctc cttcttgtct cttccaattc ccgggtcaag
     1201 gccctgaagg ccttcacctg ggccgcccac ttgtcgtagt cgtctcccct caactccccg
     1261 gattcataca aggggtagaa cgcatccgag gactgcttga acttcttctc gtagttctcg
     1321 aggcccctct gggctttcga atggttcttt tcgagttcct cgagggatgc catgagggcc
     1381 aggtccgagg gccgataaac atgtccgtgg gtggggccgc tggccgacaa gaacctctcg
     1441 aattggagag cgctcaccac gttggggaaa cgcccgaacc cgtattccga gatgcgacgg
     1501 gcgtcaaaga gctcatagcc cgtggccacc accaccgccc ccacggggac ctcgaggacc
     1561 tgatccttct gttcgtagtc gatggctccg gcctggcaga ccttggcgca aaccccgcac
     1621 ttcttgccct gcagttgccg gcaataccgg gcatcgatgg tgtaggtgga cggaatcccc
     1681 tgggcaaaat agcgataaat cgccttggtc tttcccaacc cgagattgta ttcgtcgctg
     1741 acgaccgtgg ggcacttctc ggaacagccc ccgcatcccg tgcacttgct ctcgttcacg
     1801 taccgagcct tcttgcgcac cgtggccgtg aagttcccca cgctcccttc cagtttctcg
     1861 agctccgaat aggccaacaa gtgaatgcgc ggatgtcgac cgacatccat cagcttcggg
     1921 gcgagaattc agagggcgca atcgttggtg ggaaaggtct tgtccagttg ggccatcttt
     1981 ccgccgatgc tggggagctt ttccaccaga tgaacgtcaa accccatctc ggccagatcc
     2041 aaggaagcct ggatccctgc aatgcctcct ccaatgacaa gtacgtcgcg attcaccggc
     2101 tgctctcctg cgggaactcg tacggttccc ttctgcaagt ggaccctatc gtgccccggg
     2161 aatcccgacc caatcccccc ggaccgggga acgttcccga tgcacctgga atacttccgg
     2221 aacccggtaa agcattatct gagattccca ttcccaaaag accgaccccg gcgaatccgt
     2281 attcgagtcg gaacccgtcc ggaccacatc cggctgggaa tgaatcgtgg ctgaaaagta
     2341 aacgaacccc ttcttcctcg aaaggaggtc ccgtgcagcg ttcgtcaggc ggatagaaac
     2401 atcattcatc caattgattg tgctaacttt taacggaaga caacgtgcat agtataccaa
     2461 acctgtcccg atcgtcaacg gaaacacggg acgggccgtt ccctaccatc cgccctctca
     2521 aaccccgcca ggcgtttctc gccgcggaat ccgccggttg aggaccccca cggggaagtt
     2581 tgacagacaa aaaaaatact ttgtatcatc aaacttccga tggtaataat ggctgcgatg
     2641 gtatcttgag atcccaagac ccttcccacg gaggaagcac ctttttttga tcgaaaagat
     2701 agccacattc gtgtcatgaa cggaacgagg atgttctcga gggaacggtc gccttctccc
     2761 tcaaacctga acgcaatctt tcatggacgc cgcaaggccg cgcaacgcag cggggggcag
     2821 ggggcttttc ccatggtccg agcggcaaag tccacggagg attccgcatt ttgtgcagga
     2881 tagagagcgg ttccgtttca gtcgactttt cggtagaaca gggacgacag atcggcaggt
     2941 agggcaccgg gtgccgcaca cgcacacttc attacccagc atcctgagag gagaggggac
     3001 tatgggttca gaccgaaaac tcgttttatt ggctttggac ggctcggacc aatcgttgga
     3061 aatggtcagg tacatcagcc gggtgttgac cctttccaat accgaagtgg tactctatca
     3121 cgttttccag aaggtgcttc ccatattttg ggatcgtgag caggaccccg tggccgccaa
     3181 gcacctggaa tacatggagg gttggtcggc tcacaaggaa gagaaagtgc gcgagttcat
     3241 gatgcaggcc agggatctgc tggtgagcgc cgggattccc gaggagtccg tgaccatcag
     3301 cgttcagcat aggaagcagg gcatagcccg ggacatcctg gaggaatccc gattcggcta
     3361 tgacgtggtg gctctggggc gccgaggcct ggggacgacg gacgacacta tgctgggcag
     3421 cgtggcgagc aaggtcctgc tgcacgtgag cggccccgcc gtgtgcctgg tgggaggaac
     3481 acccaagccc ggaaaggtcc ttgtgggact cgacaactcc tggggtgcca tgagggcggc
     3541 caattttgtg gcgaagctcc tgaacaaggg agagccccag atcaccctgg cccacgtggt
     3601 gcgcttcccc gccatggagg aaggcgaggg gttgaacggg agctatatcg agaaactcca
     3661 gcaagatgcc gaggacgcca tcaagccggt ttttgaaaag gtgaccaagt cgctgctggg
     3721 gttgggggtg cctgcctcca agatcggcac caaggtcatc accggcgtgg tgagccgatc
     3781 caccgccctt ctcaatgaag cccgcaacgg tcgctacggc accatcgtca tcggccggcg
     3841 gggcgtgtcc gacgtggaag aattcgagat gggtcgggtt gcggccaagc tgacccaagt
     3901 agcccgcgac atggccgtct gggtggtgag ctaatccacg gggatcataa tccacggggg
     3961 tcaactcttc tcttgtgacg agggacttct aggtgccggg gcaacccttg cccgaccgtc
     4021 ccgattctca acgggaaacg agttgacccc ttcttttttc tcccgggggc agaggttgaa
     4081 tttcaaagcc aagcgagaac ggatgtgata gaagggaaac cttttgttgc ggcgggttcg
     4141 tcggttgaga gcttccgggg acattccggc accgggaatc aagaaaccgc cccgtgggat
     4201 gccgcccaca ttcgaggcca cgatatggcg gcgaagggaa ggactgagag cttcggaaaa
     4261 tccaacatag ggaaaccgcc ccaacggggc tttggagagg aaccatgaaa atgtcgtcga
     4321 aaaggcggcg gggggccgtt ttggtgcttg ccgtcctcct gctgggcctg ggaatcgcgg
     4381 gggccgccga caagaaagcg gtcccgcctt cgggggagga aggctccgcc ggaagcctga
     4441 ccctttggaa ggccctggtg ttgggcgccg tcgagggact gaccgagtat cttcccgtga
     4501 gctccaccgg ccaccttttg atggccgagc gcctgatggg catgtacggc agggacggcg
     4561 aacctttgtc ccggcgccgt caggccaaga ccgcggcaga cgcctacgcc atctgcatcc
     4621 agggaggagc catcctggcg gtgctggggc tgtacgcacg gcgcgtgaag caaatgatcc
     4681 tgggacttgc gggcaaggac ccgtcgggct tgaggctgac cctcaacctc gcagccggtt
     4741 tcctgcccgc agcggtcatc ggtttattgg cgggggatct tgtcaagcaa taccttttcg
     4801 gtgcttggcc catcgtcgcc gcctggctgg caggggggct ggcaattctt gtcatcgcac
     4861 ggtggcagag aagatccatg gggacccgca aggaaaagga cctcacccaa ctaaactgga
     4921 gaatggccct gatgatcgga ctggttcaat gcatcgccat gtggccgggg gtgagccgga
     4981 gtctgtccac cattgtggga ggtctgctgg cggggttgtc cctcccggcc gccgtggaat
     5041 tcagcttcct gctgggcgtg gtcaccctgg gagccgccac gagccacgat gccctaaagc
     5101 atggggggga tatgctgcaa cttttcgacg caacctccat cgccctcggt ctgatttccg
     5161 ccttcgtctt tgccgtgatt tccatccggt ggatggtatc gtacctcacc cggcacgatc
     5221 tttccctgtt cggttactac cggattgcct tggctgcctt tgcgggcttt ctgatcctca
     5281 cgggaaggct ctaaggcccg tccctcaaag aaaatccccc cgaaccctct cccaccaagg
     5341 cggaggatgt tttctctcct ccctcgatgg ggaagggccg ggggccggta agacgtttct
     5401 ttccgcagag gggaacgacc cgactccacg ggtttttcgg tatctacggc cagcgggtga
     5461 tggtcccgcc ccgggagtca aacactcgaa ccggcattgc gctcctgccg tccaggcagt
     5521 gagtggcgca ggaaattcag gggtcgtagg cgcgcacgga catctcgatc ttgttcaaga
     5581 tgccttcatc caccttgccc tcccggatca gcagcttggc cgcctggttg acgctcatat
     5641 tgatggggcc gagattgtgg gtggtgccca cgatcaggtt ggcccggctg atgagaccgt
     5701 tgtcgtccgt ggaataatcg tgaatgaggg tccctcgagg ggcctccacg caacctactc
     5761 cccttcccgc ccggggttcg gcgggggtgc gcacgttggg atcgcagatg tccgattcct
     5821 cgagcagctc cagggcatgc tcataagcgt acacgtcctc gatcagccgc gcccaatggt
     5881 acagcaaggt tgcctgggcg ggcctgccga aggccgcccg aaactcctcg agctccgcct
     5941 gagcccgggg ggtggcgatc cggtccgcca cgttgatgcg agccaggcag ttggcccgat
     6001 agatgccttt gggatggtcg atgtccaggg agaacccctc gccccagcag cgggcatagg
     6061 ggaacttgcc gtaggagtac ggcacaacgg cctctcccag gtaatccgca taatcctggg
     6121 tgctgaaatc cgtgaaggtg ccgtcggcct tcatgaggcg cagcttcccc tcatagaggg
     6181 ccaagttgcc gtcctcatcc acggtaccca ggaacccggt gcggatgacc cccagagctt
     6241 gaaccgcatc caggtagttg ggaaaaacct gggtcttggc aaattccagg gtgaaggaag
     6301 caaaatccag aagttcgctc aattgcttgc gaaactcctg ccgttcctcc tcgaggagtg
     6361 cccggcagaa tcccccgacc acacccgcat ggggatgaat ggctttgccg cccacctttt
     6421 ccaggatcat cttgaccatg tagcgcatcc gaaccgcttt ggcagccagc tcgggattgg
     6481 ccttggcaac cccgatgacg ttcctcacct cgtagttcgc atcgggcccc ataacgaaat
     6541 cgggggccgc gagaaaatag aagttgagga gcttgtcctc ggcgtgggag acggtttgaa
     6601 cgaactcccg gagcttccgg gcggccggag ggatggtcac gccgaagcac cggtccaccg
     6661 ccttgttgga ggccagatgg tgatgccagg gacagatccc gcagattcgg ttcacgatcc
     6721 gagggacctc ctcggccggt ttcccctcga tgaacttctc gaatccgcgc agggacatgt
     6781 aatggacctt ggtgtctttc acgttccccg cgtcgtccag gtgaatggcg atggagccgt
     6841 gtccctcgat gcgcgtgatg ggggcgatat ggatcgtctt ggacatatcc taggcctcct
     6901 tccgttttcg ctcgggccgc agcatgccgt gagctccgga gaatctcaac agagacgcgt
     6961 attccggcag ggatttgatg tcgattccgt tggacgccag cgcgttcagc atggccagga
     7021 gctggtttcc gtcctgttgc accgggccgt aacaaccccg acagggaacc cgggcagtga
     7081 tgcaccgggg cgtgatcttg tcgccgccgc atcccgcctt ggtgaccgct cccatgcaca
     7141 gaaacccctg ctcgagcaaa caccgcatct tgtccagggg ctcgtcgggc ccgttgtact
     7201 ggggggactg gagaaaacgc ctgagctgct tcacctgacc cttgccctcc cggatggtcg
     7261 ggcactgatc acacacgctt ttgtccgcca aagagagggg catgatcccc agcaaagcgg
     7321 ccagggcgtt gaagacctgg tccggatggg gcggacagcc gggcagatag acgtctacct
     7381 tgatcttctc gtccaaggcg taaaccgaat ccagcagtgg agggattcct tccgacggca
     7441 ggttctccac tcgatcggtg gtctccgttg aatagtatcg ttggaaaatt tcttcgttgg
     7501 tgtaggaatt ggccagggct gggattcccc cgtgggtggc gcaggtgccg aagccaacta
     7561 tgatcttgca cttcttgcgc atttcatggg ctatctcgag gtgctcctcg ttgcgaatgc
     7621 ttccgctgat gatgcccacc tcggcttccg ggatttcgat gtgcttgccg tctcccaact
     7681 gcccgaaata cttgtggtcc atgagtaccg gaatatgcac gaagtcaacc aactcgagca
     7741 ccttcagcag tctttccccc aggtccacca cggagatttc acatcctgaa catgcattca
     7801 accattcttc cgctactttg actcccatag aacaacccct ttatcgccgg tgctccttgg
     7861 actcgactct ttttcccagg ggactcgtcc cgaggtctcc ccgcccccgc ccgccgactc
     7921 tcggccgccc ccggatcccc cccgaaggaa atcctgtccc gggcagtcct gcaaaacgag
     7981 taggacacgg acgggtcgcg agaacttccg gaccgttcgg ccacgggttc ggtgggggca
     8041 agggtgcctt gcctccaccg gggagggcgg tgcgccgtcc ccggggaggg tgactggggg
     8101 cctcgggctc atgaccgccg ggtcaggcca ctttcctctc tcgcagaggg ttgggtccca
     8161 gttctcgtat gtcttcgatg aaacgattga cgacttctgc aaacttgggt gcttccgcag
     8221 aggacaccca ttgtgctcgg aatcggttgg aatcgattcc gcaactatcg agaaccacct
     8281 tgacgacatc ggatcgggcg gacgcttttt gattaccttc caggtaatgg cattctccca
     8341 ggtgtcaccc gaccaccaga acgccgtcgg ctcccttgga caacgcgtga accaccaggt
     8401 cgggactgac cattcccgag cacatcacgc ggatgatcct caaattggtc ggatactgca
     8461 aacgactgac cccagccagg tcagcagcgg catacgctca ccacgtgcac aggaatccga
     8521 ggatgaccgg tttgaaagga tcggacatga aggatctcct ttctgccaac atgaatcgtc
     8581 caaatggaat ggggaagggg cccggatgct agacggcctc gagtgccgca tcgacctggg
     8641 ccttcaactg atcgagggtg aagccgttca cctccacccc tcctttggga cacgtagcgg
     8701 cgcacacgcc gcatccctta cagaggattt cgtcggatac gatcctcttt acctttcggc
     8761 catcccgttc gtactcctcc aacttgatgg cgaagtaagg gcatacgtcc acgcacaggg
     8821 cacagccgtc gcagtgttcc gtgacccggg agatgagggc gctggagatc agcttgtcct
     8881 tgaaaagcac cgtagcggcc cgggatgcgg ctccaaaccc ctgggcctgg gcctctccca
     8941 ccgtagccgg gtagcgggcc gtcccgcaaa ggaagatccc gtccgtggcg aagtccaggg
     9001 gacgcagctt agcatgggct tcaaggaaaa accggttttg gtccacgggg atgcgcatga
     9061 ggttcgccag agcggcggca tcgtcccgag cgaccagggg agtggagaga accaggagat
     9121 caaaggggat ttccaggacg tctcccgtaa gaggctgaca aacctctacg gtgtttcctt
     9181 tcaccaccgg cgggcagttc acgtcgtaga cgtcgtagcg aacccccttt ccccttgatt
     9241 cccaaagcat tcgttcgttt tccgtgccgt acatttgaat gtcccggtac aggatgtgaa
     9301 cccgggcttc ggggttctgc tccttgatgt aaagggcatt cttcacggcg gtgagacagc
     9361 agatcctcga gcagtattcc cgctcgggga cccgggatcc cgcacactgg atcatgacca
     9421 ccttccgggc gttgaacgac ccgtctcgga gcatctcttc gagttcgatc tgggtgacca
     9481 cggtcttacc gtcatagccg tacattccct cgggtttgag ggggacggcc ccggtggcca
     9541 ccaggatgca gccgacggtt tcctcgatct ctttgccttc gccgttgcgc agggtcacgt
     9601 ggtagttccc gatgacccct cccaccgaaa gcacttccgc gcccagcaga gtctggatcc
     9661 ggggggttcc cttcactctc gagaccaact tttcgaccac gtccgaagcc ttctccccgc
     9721 cgggagccaa ccggttgatc cggttgagga gtcctcccag ccgggcttct ctctcggcca
     9781 ggaccacctc gacccccatg tcggcaaggg tgcaggcggc cgacagaccg gtgactccac
     9841 cgccgatgac cagggccttt cgaaccaggg aagattcgat gtccttttgg ggagccagga
     9901 ggcttgcctt ggccacggcc atccggatta ggtcccgggc tttcaccatg gcatcttccc
     9961 cttgctgcat gtgaacccag gagcactgat cgcggatatt ggccatctcg aacagatagg
    10021 ggttcagccc cgcctcgtcg caggcgctct tgaaaagggg catgtgggtg cggggggagc
    10081 aggaagccac caccacccgg gtcagttgat gctcgccgat ggctttcttg atttccccga
    10141 tccccgattc ggaacaggta tagagattgt tctttgtgta gacgacccct ggaagccccg
    10201 cagcgtattc cgtcaactgc acgcagtcaa gaagtccggc gatattggag ccgcagtggc
    10261 agacaaagac cccgatccgt gcttccttgg cttcgggatt tacgaccatt tcaccattcc
    10321 cactcatgga cctcttctcc ctcaggacgc gagacatgcc gattcggaac ccgacaggac
    10381 ctgagccgcc cttgccgccg cgctgctggc ctgggccacg gattcgggaa tgtccatcgg
    10441 actgtgagca caaccgcaga catagatgcc gggacgggtg gtatccatgg gaggataacc
    10501 aggcgtcttg aaaaacccga agtcgttgac ctcgatgttc aacaactctg ccatccgttt
    10561 ggaggacgca gcgggttgac aagcggtgga gagaaccacc aggtccgccg ccagctgctt
    10621 gacccggcgc tccttggtgt cctcgtacca taccgtcagg ttgtggtccg cgtcctcggt
    10681 gacctcggcc actcgactgc gaatgtattg aattcccgac tctcgtcccc cgcgatttcg
    10741 atactcctcg aagccctttc ccaccgcacg tatatccatc cccaggatga acacctccgc
    10801 ctgccggtcg tgctccttgg cgatgatggc ctccttgatg gcgtgcatgc agcaaaatcc
    10861 cgagcaatag cggttgaagc ggatatcccg agacccgacg cactggatga acactaggcg
    10921 gtgagccgtt tcgatcttgt ccacccgctc ccgcaaccct tcaaactccc cgtcggcctt
    10981 gcgggcggca cgcacttgct cggcccattg gtcgtaatcc tccccggcaa gttccccgga
    11041 ttcgtagagg ggaaagaatt cctccgaggt gcgatcgaag gtcttctcaa acttttccag
    11101 ccctttccgg gccctttggc gggccttttc gaggtcgggc aaggcggcca tcaaggccag
    11161 gtccgaaggc cgatacacat gaccgtgggt gggaccgccg gccgaaagaa accgctcgag
    11221 ctcgagggcg gtgatcacgt tgggaaggcg gccgtacccg tattcgggaa tccttcgtgc
    11281 gtcgaaggtt tcgtaacccg tggccaggat aacggcgccc acctccactt ccaggatttg
    11341 gtcctgttgc ttgtagtcga tggcccccgc ctggcagatc ttgctacaga cgccgcattt
    11401 cttccccggc tggaaatttc tgcaatattg ggcatggatg gtatacgtgg aggggatccc
    11461 ctgggcgaag taacgataaa tggccttgga ttccgccagc cccaggttgt attcgtcccg
    11521 aacgggagtg ggacactttt cggcacaggc tccgcaaccc gtgcacttgt tttcgtctac
    11581 gtatcgagct tttttgcgga tggtgacccg gaattctccc agctccccgt ccaccttttc
    11641 cacttccgaa taagccagca accgaatctt gggatgtcga ccgacatcca tcaacttggg
    11701 ggccagaatt cagagcgcac agtcgttggt ggggaacgtc ttgtccagct gggccatttt
    11761 cccaccgatg gtgggcaacc gttctacgag attgacctgg aaccccattt cggcaaggtc
    11821 cagggatgcc tggattccag cgatgcctcc tccgatgaca agaacatcac ggctcatgat
    11881 caggtactcc ttcagctatc ggctttcaac ggtcaacttt ccggtatgtg ctccatcgcc
    11941 atggggtcat gcggactgaa gccgaaggca aatagccgtc attccatacg ggtgagtaag
    12001 aaactgtaca ggtccacgat attcaggttc agatccgtgt tgctctgagc gcacgtgagg
    12061 tgaatgcggc acttgggaca ggcggtaacc agggtggagg cgcccacgtc ccgtgcttct
    12121 tcgagtcgtt cgatctgcat ggtcttggaa cagttcgagc attcgatcca tgcgctggta
    12181 ccgcagcaga gagcgttttc gcgattccgc cccatctctt cgaagaccac ttggggaagc
    12241 atcttcagga gatcccgggg gggctcgtag atccccgcca cacgtcccaa cctgcaggga
    12301 tcctggtagg tcacctttcc gtttcccgga tcctcggcgg gtcgaaaggc cacgccggag
    12361 ccgggaagct cccgggcaaa gaactccgtg gtgtggagca cctcgaaggg aagatccccg
    12421 aaaaatttgg ggacatggta cttgtaagtg gcgtatcctt cggggcagct gaacacgacc
    12481 ctcttggccc cggagccccg gatgacctcg aggttcatgg aaaccagccg acggaacagc
    12541 tcttcgttgc cgctccacag ggcgtcatgg ccgcagcacc gttcgtcgtc gctcatcacg
    12601 ggctcgactc ccatgcggtt gaggagacga agaacgctct tggccgtctc gagggaggtg
    12661 tctccgtatt tgatgaccgc atcgaagtag ggagcgcacc ccacgaagta gaacgtgtcc
    12721 cccgaggcct ggaatttgcc cacgtcccgg gcccagtcgg tccgcttctg cttcacgccc
    12781 gtggcctgca gttggacgat ggcctggagg gtcccgtgat ggctcagctg ggggagattc
    12841 ccctccttgc gggccagctc ccggtgaatt cgagcgaaat ccgggaaatc gatctgtacc
    12901 ggacagcggg agctgcaccg tgcacaggtc aggcatgccc agagctcgcg gctctgaaga
    12961 accgtctcgt cccggtccat caaggtgcgt ttgatcatct gtcgtggaga aaacgtcggg
    13021 atcactcgac tgatggggca gctacccgta cacactccac attccataca aaaatcgatc
    13081 tgctgctggc tgagtatcat gtctcattcc gtggtttcgc ttagccgttc aatgcttccc
    13141 tcatccaaag ccgggtgggg cttccaccgg tttggacctc gtccacaggg gctcaagccg
    13201 cgctgccgac ctcggacccc ccctggccgg gagccgaatc cgtgagggga tttttcccca
    13261 gctgcttcaa aaggttggtg aaagtgcgga tctcttcggc aaaaatgccg ccctcggacg
    13321 cagagatcca tttgagacgc agatatcgct catcgactcc aaacaggttg agtaccttgt
    13381 gggtttcctc gaccaccttc tgacacttta cattacctcc caggtaatgg cagtctccga
    13441 tgtgtcagcc ggaaaccatc acgccgtcgg cacctgccat gaaagcattg agcaggaagc
    13501 cgtggttcat cctgccggag cacatgaggc gaatcagccg gacgttggga gggtactgca
    13561 tgcgcgccac ccccgccaaa tccgcccccg cataggcaca ccagttacac acaaaggcga
    13621 caattttggg ttcgtagttt tcggacatcg gctttcctct cagttcccgg tccccggctt
    13681 tgggaaccat gcccccggcc ggaaacccac cgccgttaca gggcccgtat cacagcattg
    13741 atctgtttat ccctaaagtg ggcgagggag gcggctccgg tgggacaagc cgcgttgcac
    13801 gcaccgcagc ccgtgcacaa ggcctggttc accgtcgctt gtcttcgccc tccttccacc
    13861 tctttcacct tgatggcctc gaacaggcag gtctgctcac attcgccgca ggcccggcac
    13921 agttccccgt tgacctgggt gatgacccct tcggccacga tttccttctg ggacaggatg
    13981 gtcacggccc gggatacggc tcccttcgcc tgggcaatgg cttcgttggc gaacttggga
    14041 ccttgagcca gcccggccag gaagaacccg gccgtggcaa aatccagagg ccgcagtttc
    14101 aggtgggctt ccaggaagaa cccgtcctga tccaggggga gccgcatgac ttccgccagc
    14161 ttcttgctct cggctcgagg ccgtatggcg gcgctcaata ccagcagatc cgcatccagt
    14221 cgaacccggc gcaacagctg ggcatccatc atggaaacct gcacactgcc gccgctcacg
    14281 tccacctcgg gcttctgatc ctcttcgaac cggaagaacc gaactccccg ggtgcgggct
    14341 tcctggtaag ccaactcctt gaatccgaac gtccggatat cccggtagag gatggaaatc
    14401 tgagcctgag gattaagttc catcattttc aggctgtttt tcacggccga ggtgcagcaa
    14461 acacggctgc aataggagtt ctccggctcg cgggagccca cgcactggat catgacgacc
    14521 ttccgaattc cccgggcctt gtcgggctcg gtactgagca tggcctcgaa ttccttctgg
    14581 gtcacgatct tgggatgctt gccgtactgg tattccgtgg gtttgtattc ctcgccgccg
    14641 gtagccacga tggccacccc gtgggagatt tcccgggcgg ctccattgac ggcgacgttg
    14701 gtgacgaagt tcccgcaact tcccatgacc gacgtcactt cggcgttctt gaggacggtg
    14761 atcaagggat tggattccac tttctcgatg agctcctgca cgtaccgggc gggattggcc
    14821 ccgtcctcgg tgtagtgaag ggtccgggcg tttcctccca gttccgccga ccgttccacc
    14881 acgatggcgg gaaagccctg ctcggcgatg gagagggcgg cagtgagtcc ggccaacccg
    14941 ccgccgatga ccagggcctt ctgaaccacc tcgtaggtaa agtcgtaaac cggctcgagg
    15001 agactcaccc gcgccaccgc catgcgcacc aggtccttgg ccttgttggt ggcggccacg
    15061 ggatcccctt ggtgcaccca ggagtcgtgc tcccgaatat tggccagctc aaagagatag
    15121 ggattcaatc ccccttcccg cagggtgttg cggaacagcg gttcatgggt tcgaggcgtg
    15181 caggatgcca cgatcactcg attgagtttg tactcctcga tgattcgttt gatgtccttc
    15241 tgctggtccg tggagcaggt aaacatgctg tgggttgcat actcgacccc gggcagggac
    15301 cgagcatatt cggccaccgc ctccacgtcc acgacccccg cgatgttgat gccgcagtgg
    15361 caaacgaaga ctcccacccg gggcttctca ccccggatat cccgttcctc gtattgggga
    15421 ggaggcgtga tgagagtgcc tcgggcctcg gacagaaggg ccgtggcgca ggatgcggca
    15481 ctgctgccct gctggaccgt atccgggatg tccttgggac cctgggacac tccgcagacg
    15541 aacaccccgg ccttggaaga ggccaccacg tccaggggat ccgtggcggt aaatccatgg
    15601 gaagtgagct ccactcccag gcgtttggcc agttccgcag tggaaggatg ggagcacagt
    15661 cctacggaca gcaccaccat gtcgaattct tcctcgcgaa gctcgttccg ggcgtcggaa
    15721 tagcacaccc gcaggcggtc gctctcggga accggcacca ccctcgaaac catggatcgg
    15781 acaaaccgga ccccggtttc atcccgggcc cgctcgtaga aacggtcgaa ccccttcccc
    15841 tgggctctca tgtcgatgta gaagatggtg gtctcgatgt cggcctcgtg ttccttggtg
    15901 atcatggcct gcttggtggc gtacatgcag cagacatagg aacagtaatc ccggccgatg
    15961 ctcttgtccc gggagcccac gcactggatc caggcaatcc gctggggctt gcctccgtcg
    16021 gaaatccgct ggatgtgccc gtggaaggga ccggctgccg agaggattcg ctcgtattcg
    16081 agactggtca ccacgttggg ccagagcccg tacccgtact cgggcttcag cctggcgtcg
    16141 aagggcttga atcccggagc gaagatgacg gcccccgcct ggatctgttc gtagccgccc
    16201 tccatttggt ggtcgatggc cttggccata caggcgtcca cacattggag gcattcgcaa
    16261 caggtcatgc agttcaagca cttccgggcc tcggccaggg cctgctcttc ggtaagaccc
    16321 agttcgactt cgccaaaccc cgccttgcgc ttggacatgg gaatggtgcg catctccgcc
    16381 cgcggttgga tttgcgcatc ttcgggaatg ggacggaagt tctcctggga gacctttccc
    16441 ttgggctcgc gcccggcctt caagtcctct cccttgaggt agcgggaaat ggaaaccgcc
    16501 gcctcccttc ccgccgccac ggccccgatg gccacccagg gacccgtttg ggcgtcgccg
    16561 cccgcaaaga ccccgggacg actggtggca aaagtgattt catcggcttc gatggttccc
    16621 caacgggtga gcttcaaccc ttgggactcg gccaggaaca gggaatcggg cgtctgaccg
    16681 atggcgggaa tcagcatttc cgcctcggag gtgaactcgc ttccgggaac cggaatgggt
    16741 ctgcgcctgc ccgaggaatc cggggcgccc agctccatgc ggatgcactg gatgccgatc
    16801 atcttgccgt cccgggtgag gacctgctgg ggggccgtga ggtaccggat ctcgacccct
    16861 tcggccaggg cgtcttcgat ttcgttttcc cgggcgggca tttcctggcg gctgcgccga
    16921 tagaagatgg acaccttttg ggcccccagc ctcagggcac accgggccgc atcgatggca
    16981 acgtccccgc cgccgatgat gacgaccctc tgtccccgga tatcgggggc ctgccccagg
    17041 ttcgccttgc ggaggaactc cacgccgtgc atcaccccct cggcgtcttc cccggggatg
    17101 ttgagcttga gactcgagtg ggccccgatg gccaggtaca ccgccttgta gccctgggcc
    17161 tccaggccgt ccagggtgat gtcctttccc agcctttgat tgtaccggat atccaccccc
    17221 aggcgggtga tgtagcgaac ctctttctcg aggacttccg gaggcagccg ataatcggga
    17281 atgcccaccc gcagcattcc gccggggacc gaaagggctt cgaaaatggt gacctgaaag
    17341 ccctccaagg acaggaaatg agcggccgta agacctgcgg gccccgaccc gatgacggcc
    17401 accttctcct cccgctttgc gacctcggga accggccact gctcgatgtc caccttgtcg
    17461 gccacgaatc gtttcaaggc gcagatggag acgggagcat ccacttcccc gcgacgacag
    17521 gccgtttcac aagggtgggg acagatgcgg ccgatgacgc cgggcaaggg gagattgcgc
    17581 aggatcactc ccatggcatc ctggtagcgg ccctgggcga tcaacgccac gtagccgtga
    17641 gcgttcaccg agttcgggca tgcgttggtg cagggagact tgtccccctt gtcgatgctg
    17701 taggtgggag gaatggcctg gggatagttg cgatagatgg ccttgcggct gttcaaaccc
    17761 aggttgaagt tggcgggaag atcgaccggg cataccttct cgcactctcc gcaccccgtg
    17821 cagcgatcct cccgaacgta gcgcggatgg cgcttcacgg tgagggtgaa gttccccggc
    17881 tccccgctga ttccctccac ctcgctcttg gtgaggatct ggatattgcg gtgattggcc
    17941 agctccacca gctttggccc catcatgcag gaagaacagt cgttggtggg gaaggtcttg
    18001 tccagctggg ccatgacgcc ccccacgttg ggttgttctt ccaggagata taccttaaaa
    18061 ccggagtttg cgatatcgag cgcgctttgg ataccggcaa tccctccgcc cacaacggcg
    18121 actgcaccga ccttttgttg tgccatgacg ctctttcctt gctgtgacat gagccgttca
    18181 ccctctcgaa gtttccacga agctcgggcc gggacccgaa tcgccgacga ttcagatgcg
    18241 tgccgcccgg agtgccggcc gaggatccgc aaaatgccgg gacatccagg aggtggcctc
    18301 ctcgatgccc aaggccaggc cgatgagctc cgtaaaatac ggcacgggaa gggcaatggg
    18361 cctcccccag tctttttcga tcttttcctg gtacatatcc aagttggact ggcacatctg
    18421 gcaggaaacc accatggcct gagcccccac tctgcgggcc atgtcaaaga gctttcctac
    18481 caggcgggaa cccatatcgg gccgggacaa catgagactg gctccgcagc agtccgtctt
    18541 gtagggccaa agaatgggtt tggcacccaa agctttcatg atcttgtcca aactctgggg
    18601 attctcccag tcggtggatt ccgtgacgga gggaggccgg cttgcctggc acccgtaata
    18661 gcagacgacc ttgagaccct cgaggggttt cttgacccgc tccttgatct tgcccagcat
    18721 gtcgtccgtg gccaggaagt tggcaaggtc ccagatcttg ggagtggaag catccagggc
    18781 catccggtat tggtgggcct cccggcttcc caagacatgg gaagcggttt tcaggcggtt
    18841 gtagcagagg gggcagggca ccaccatgtc tttgttgggc acccgggcgg caagtttgag
    18901 gttgcggccc gccagctcga tgctcgccct gtgatggatg ctgtgagcgg cagtggctcc
    18961 gcagcaattc cagtcgggca cctcctccaa tgtaatcccc aacgaggcac agacgccgcg
    19021 gatggaatcg gcgtaatccc gggccgtgcc ttccaaagaa caaccaggat aataagtcac
    19081 attcatcatc gggttcccgt tcagtggcaa aagatggacc gatggtcgcc ggattcagga
    19141 aacacaaccg cgcaaccccc aggaacattc ctcggcgagt cctcacgaag accggagaag
    19201 gtcccgaacc tcttcctttc cctcgatgac cttgggaacg aggggcagtc gatccttttt
    19261 gtacagcctc caggccattc gcaaatcgtc gcgccatgag ccttctcgaa ttttgctgaa
    19321 catctgaccg cttcgcaggg tgtagagcgg gaccaacccc ccttcgaaaa ccctgcccgc
    19381 gtactggacg gtctccaaga aagcctgatg gagggccaag acctggggct caggcgacgg
    19441 gatggattcc cgaacggcca gctctttgaa gtaatccatg agctccgcga tgcgcacgcc
    19501 gttggggcac cgggtggtgc aggtctcaca ggacgcacat acccagatgg tcttggaacg
    19561 gaggacctcg tccttttgtc ccaggatggc caatcgaatg acctgatcgg ggtagagatc
    19621 catggcaaag gtggcggggc agccgtttgt gcatttacga cactgaaagc attgagccgc
    19681 gtaaaaaggg cccgcggcct cagctttctc tagaaaggaa aaatcgggat tcaaaacggt
    19741 ctgctgcatt ggcgtggcaa cctcctgcag ttgaggtgag gaagctttct ttgcgtctac
    19801 gtcccatcct cgaaggtccc atccccggca aggaccgaag atacgggacc ccatgggctt
    19861 tcacaccggg gaagaggctc ccgggggagg acgggcgccc catcggctcc ggtcccggaa
    19921 cccgtttggc ccccgcaggc cgccgaagtc cctcaagatt ccgtcccggg cttcaaaccg
    19981 ggtatcctct tccccttcaa gagaaaccga cccggacctt tccccgccgt gagggggtca
    20041 ggtcccccgg aaaccgtccc cgccggcgcc cccctcggag ctccgggccg ccccgggcct
    20101 ggtgccccgg cggaccggga cggacggttt cctttgggat atcgccttgg ttcgggaatt
    20161 taagcacacc cgtgtgcaat ttgtaaatag tatagaaaga ccagcatggc aaacaatttt
    20221 ttataacatc aaacatccgg cgggggatcg gcaggggcgg accttgatgc aaccaatgga
    20281 tatcattgcc tatcgtgact ttgccatttc caaccgactc aaataaccct acaataacat
    20341 gtttggaaaa ggatcccgac tcaggtacaa aattcccaaa cggaccccgg ggaaagggaa
    20401 acccagccca tgaccgttgt ggccattatc ggcagaccca atgtgggaaa atccaccttg
    20461 ttcaatcgga tcgccggaag gcgacatgcc ctggtggacg actttcccgg ggttacccgg
    20521 gacaggaact acgccccggt atcctggcgg ggaaagactt ttaccctcat cgacaccgcg
    20581 gggttcctgg attcgaccga atccggtctc gaggagcagg cccgggagca gatcctgcta
    20641 gccctggacg aggccgacat tctccttttt gttgccgacg gcaatacggg gctccatccc
    20701 gaagaccgtt ccctggtgag gatgctccgg cgatcctcca aaccctccta ttttgcggtc
    20761 aacaaggtgg acggccccga aaaagcccgc cttctgggtg aattcttcga actgggcctg
    20821 gaaaagatct accccatcag cgccgctcac gggtacgggg tggcggacct tctcgcggac
    20881 cttacccggg ccgttcccga cgccccggaa accaatgccg gggaggatga ccccggtgaa
    20941 atccgcctgg ccgtcgtggg gcgccccaac gtgggcaagt ccaccctggt gaaccgattg
    21001 ctgggagctc cccgggtcat cgtcagcccc gttcccggca ccacccggga tgccgtggac
    21061 tctcccctcc ggtggcgcaa ccggtcctat ctgctcatcg acaccgccgg gatccgacga
    21121 aaaggcaaga ctcgagagaa actggaaaaa atcagcgtcc tcaaagccct gcagagcatc
    21181 gagcgaagcc acgtgagcct tctcatgctg gatgcctccg aaggcattac cgaccaggac
    21241 cttcatattg ccgggtacat ccaggaccaa tcccgtgcct gcctggtgat cctcaacaag
    21301 tgggaccttc tggcagcgga cccgaagcgg cagaagcgct acggggaaga ggtccgggac
    21361 cgcatgaggt tcatgccctt tgcccccctc ctccccgttt ccgcccagac aggtcgcaac
    21421 ctctccaaga tccttcccac gaccaacgaa atatttgaac aatataatcg caggattacc
    21481 accggggtgg tcaaccgcgc cctggaacgg accctggccc gccacgaacc cccgtcggcg
    21541 ggaaaccgtc gcctcaagtt ctactatgcc acccaggcat ccacccgccc cccgaccttt
    21601 gtgctttttt gcaacgaccc ggaatccgtt catttttcct acaagcgcta cctgatcaac
    21661 cagttcaggg aatcctttca gctggacaag acccccattc gaatcctgtt tcgcgggaga
    21721 aaatcggact agggttcctc cgccgtctgc ccgggaagcc tcaggattcc atccgcttct
    21781 tcgatcctgc cccttttccg gcacccgtgg gcgtagatgc ggcatctttt ggcgagctcc
    21841 gccaaagcca gggtcctttg atcgggggtc aggggggttt cccgaagaag cttgacaagg
    21901 gcctgaatgc ggtatcgatc cagagcgggg atgcgggcgg agagttgatc ggagtgcccc
    21961 ccgcgcttga cgaccaaggg ctccctgacg aggccgaagg ggtatcgcgc tcccgtccgc
    22021 agccagaatt cgtagtcctc acaggcggga aaatcctcgt caaaaccgcc gatttcctcg
    22081 aagaccctgt gatggatcat gaccgcggag gggctgatca aacaacgctc caggaggcgg
    22141 gggaaacaat ggccttcggg ctttgcatgg catttccggg ggttgagtct ccgcccgttg
    22201 cggatccaga tttcctccgt ctgacaaagg agaagcccgg gggaatcggc cagaaactcc
    22261 aattgcttta ccagcttctg cggaagccac aggtcgtcgg aatccaggaa cgcgatccat
    22321 tctccccggg ccagggccag tcccgcgttt cgagcggaag cgacccctcg gttgtcctgg
    22381 cagacggttc gaaggcccga cccaagccgg gaaagccttt ccctcgtatc atcggtggaa
    22441 ccgtcgtcca ccacgatgat ctcgaaggaa acccctcccc tctgggccaa aaccgaacga
    22501 acggcctctt ccacgaacgc agcccggttg taggtgggaa tgacgacgct gaccttcatg
    22561 cccgaacgat gcctcggggg agagaatccc ggcgcctcac cactggacca cgcgcacatt
    22621 ggaaagggag cgccccagaa aacgctctcc gatggactgg aatcgcaggg gcacgtcggt
    22681 cacgtagaag cggtactcgg ggacggcgcc ctcccgattc tcgaggtccc tctcctgcag
    22741 gacggcagcc gtctgttcgg ccatggcttc ggcggaatcc accaggcgaa tgtccggtcc
    22801 cgccacttcc tggagaaggg gtttgaggag ggggtagtgg gtacacccca gcaccagggt
    22861 gtcgatggag tggcaaagga ccggtttcag gtattcctcg gccgtcagcc gcgtcaccgt
    22921 gtggtccagc cacccttcct ccaccagggg cacgaacagc gcacaggcct gggagaagat
    22981 ccggatttcc ggatcgtacc ggtggatggc ccgggcatag gcattgctgt tgatggtcgc
    23041 cggggtcccg atgaccgcca cggatttgac ccgggtcccc gcagcggcct cccgcgctcc
    23101 cgcatcgatg acgtccaaca ccttcacggc ggaaaggtcg tccactacct ggtaggctac
    23161 ggccgccatg gtattgcagg caatgatgag aagcttcacc tgctgttgaa ggagaaattc
    23221 cgtgatctgg cgggcataat gggcaatggt ttccacggac ttcaccccgt aaggcacccg
    23281 agccgtgtcc ccaaagtaga cgatgttctc aaaggggagg cgttccatga gagcgcgcac
    23341 taccgtgagt ccgccgatcc ccgaatcgaa aaccccgatg ggattggtgg gcttctcctg
    23401 catgcatcct ccgtggggag cagtcaagaa cccgaaaggt cgagaggacc tcgaaacctt
    23461 ttcctgggcg ggtcctcaaa ccttatggag cacccggtgg agcacttcgc tcaactgctc
    23521 gattcggtaa ggttttgtca ccacgccgca aaagccgtac cgttcataat gggccatgac
    23581 gggatcgttg gaatatccgc tggagaccac ggccttgacc ccgggatcga tctcgaggag
    23641 cttcttcacc gcaattttcc cgcccatcct gccggggacc gtcaggtcca tgatcaccgc
    23701 atcgatgggc cgtccggcct tgagagcgga ctcatagatg cggatggcct cttccccgtc
    23761 ccgggccgcc tcacactcgt accccaggta ttccagcatg ccgcacacca cggtgagcac
    23821 cagttcttcg tcatccatga gaagaacccg tccccttccc gtccgcgctt tgacgtcgaa
    23881 gttccggcac tccgggaccc gcccccacga agcgggcagg tagacctgca cctccgttcc
    23941 ctcgttcaac cgggagtcca cgataacgta cccgtcatgc ttcttcacga tggaatagac
    24001 catggtcagg cccaggccgc tgcccttctg cttggtggtg aagtagggat cgaacacctt
    24061 ggacaggagg tcttcgggga ttccaatgcc ttcgtccttg atggagagga aaacatatct
    24121 tcccggagga attccctggg ggtttttctc ctccaggatc cggttgccgg ccgccacacg
    24181 cacggtaccc ccgtcgggca tggcctggtc ggcattgatg atcaggttgt tgaccacctg
    24241 gctcatttgc ccctcatcca cctcggcggg ccacaaatcc tcctggatgg caatgtcgca
    24301 ccgaacgttg gagccgcgga gggcaaaggt agccgagtct cgaatgaggg aggccatgga
    24361 gagggttttc ttgatggggg cccccccgcg ggagaaggtc aggagctgct gggtcaggtc
    24421 ctgggccctc aaagaagccc tttcggcctc ctggagcttc aggaaaagag ggcttcgggg
    24481 ctcggcaaat tgcttggcca gggaaatgtt gcccaggacc gccatcaaga tgttgttgaa
    24541 gtcgtgggca atccccccgg ccaaaacacc gacggcttcc atcttctggg tctgttgcag
    24601 gagggtttcc atgcgcttgc gttcgctcac gtcgtttaga aggacgatgg cccgtcgttc
    24661 ttcttggatg gggatgaggt tgatggaaat ctgcttgttc aagatcttta cgggggcacc
    24721 caaagaaatg acggcggtgg acccgttggt ggaatccagc aggccctgga cccgttcccg
    24781 gtcttctgcg ttgaaaagct ctttgaagtt ggagcccaga atctgctctt ccggccgtgc
    24841 gatgaggtcc tttgccaccc ggttcaggta cacaatcctg gcttcccccg taatctccag
    24901 gattccctcg gccatgctct ccaggatcag ctcgaaatgc ttcttttccg tcaggagctc
    24961 ccgggtaatg gcccgggggg taaagatctt tcccagaacg gggctcttgg gaggatggcc
    25021 cgcctccacc cgctcggatt catccagagc cagttccacg ttcgcggcca tttcgtccaa
    25081 ggggcccttg gcaatacagg cgttggcccc cagcttcgcg atgtccagct cttcttcggc
    25141 cgccacggcg gaaagaatca cgatgaagag gtcccggaaa ccgggcattc gccggatgat
    25201 ctgacaaagc ttctttccgt ctatgttggg catgatcaga tcgatgaaaa cgacctgggg
    25261 ggtaaactcc tgcagaatat ccagggccga aaggccgtcc tcggccaccc tgacgtcgta
    25321 gcctctcctg gtgagcaagg tcgacatgaa tttcaggatc agaggatgat tgtccactac
    25381 cagcactctt ttcttcatac gctccaacct cggcaacagg aaaccggcat attcaaacga
    25441 ctacgaaacc tccgcctccc cagttcaccc gcatgtatcc gtcatgacgg gggttcggtt
    25501 tttggctccg ggccctgagc cttgaagcct cccgccgggc gaggacatcg ttgcggcaaa
    25561 atccttccct gaaagccctt ccccctcgga cccctccccc agtccgccat gtcgatctct
    25621 cagttcgggg attcgcctat attatcgggt cctttgccca acaactttat agtttatctt
    25681 tcttttcgat cgttgcggcg aggcgttcca catcttttcg aatggcggca acggcttcgg
    25741 tacttccttc gaggatgttt ttccgggcgt tgtccacaag aactttggcc atgtcttgaa
    25801 tactccggat ccccagggtc cccgccgctc ccttgaggga gtgggcgcat tcgatgagcc
    25861 tcttcaggtc cccttgtgca acggcttgtt cgaggaggtc caggtccgtg cgggtagcat
    25921 ccacgaagat ctgaaccagt tccagaaaat ccttttcttc gaatcccagt ttctccgcca
    25981 ttccttttag gtccatgcat cgactccaac gcgaatcaag cccccggcct gcaaccctgt
    26041 gcccgccggg tcccgtgtcg cccggcgggc ttccatcctg tcccgcattg ccctcgtccg
    26101 cggttccccc gaaggaatcc gccgggaatg ctctgccgaa gtcgatcaga ctcttgggga
    26161 atgcagcgca acttatcata ttcctaccga acttccaggg gattttgggg tcccaaagag
    26221 ctctgccccc cgcgaagcac tgggtttggc ttctcggctc acggccggga agagcctgtg
    26281 ccgcaccggc tcaaatcctc ggagaaagag cagcttggaa ggattctctc cgggaccctg
    26341 cgaaagaggg aaggaccttg ggaaaaagtg tggtatgctt ttttttcgcc ggacatggaa
    26401 ggaacccgac cttatccatc ccatcgacct gcggcggtgc catgtatctc agctcattca
    26461 tccaccggga agagctcttc cacatcgcgc agcgctggtt ctgcggccgt atggaaccgg
    26521 atgacgcttt gaagctcacc cagatcctca tctgcgacgg gtttgtgctg gggcaaaccc
    26581 tgcaaagggt cacccggacc ctgttgggac ggatttgcgc cgattgcatg cacgaggaac
    26641 ccatccggat caaggggcaa ttgagggatg ccctgtgtcg aagccctcga gttttcaccc
    26701 cccggacccg ggagctgtgc agggaatacc gggcaaatcc cgaatactac tatgcggaag
    26761 tgcccatcaa cggcgcactc tacacggact cgaaccacgc cttgtggggg gtctaccgga
    26821 tcaagaggcc ccggcggatt gccgaaaaag ccaaccgcaa gatcgcccaa tggatcttcg
    26881 aaagggttcg gcaccgggcc caggagatgg ccctggagcg cgcccatcgc tacgacattc
    26941 ccctggaccg gctggttact cccgagaatg aaatggtgga agagttcatc caggctgaaa
    27001 aggtcatcgc ccgcagcttc agcgacggaa cgatgtccct ggacccaagc gtcatgacca
    27061 ttcacgacgt cgggggaatc aaggtcgtcg ccgaccggga agacctggat ttcctggaaa
    27121 agagtctttc ggcgaactcg gactggaagg tcctcgatag ggagaatttt cggggggact
    27181 accgggccgt gagcctcatt ctccaggtcc cgtgggaacg ggaagaggtc tgcaggcaat
    27241 accgggagac ccggagctgg aggaagtacc tgaatcgggg aatcggggaa gacgaattga
    27301 agaaaggcct ggagccgctc ctggagaacg ccgaggccca cctcaacctc gagctcatcc
    27361 tgtccacgtt cgaggatctg gtggaatccg agctgggaaa ttccatccat gaagaaagga
    27421 tcaccgccca gagggaggcc cgggtctaca agggctacat ccccatgaac gtggaattcc
    27481 tcctgggtta ccttttcgcc gtgggcttca gcccccgggt tcgaatcgac tccctgcccg
    27541 tccgactgtg gggccgatac ctgcccgaca ccctcctttc ccactttcgt cggctgtatt
    27601 ccctgccgga atacgatctt ttgtcctgac ggggaagccc ggcaccgccc gggaggcgac
    27661 tcgggcggac ggctaggggt caggtctccc gatgacccat tgagacgttt caatccaccc
    27721 cgccgcgagg ggggcgaccg aggttcctgc cccgttctgc ggccggtccc ggtttcaatc
    27781 catgcccccg cgcgggggca tggatctccc tccggcagat tcgaggctgc tttttcgatc
    27841 aaaacatgga tgtcttcggg ggctggaatg ggccggtgcc cattgagacg aacttacaat
    27901 tcaagccgaa accgaccacc cgcaggtttg agccgttttc tcgaaatctt cactcggcct
    27961 gtcggagcga ctatgggcgc aacgtaatgc cacacgagcc gcggtgcctt ccctccggga
    28021 aactgctcgg actccgggaa cgacgggggg atgagaaaag gcggcccgga ggccgccttt
    28081 tctctgcgtc ggggccactc gatgggtgat tagtcgcgga gcggattgcc tttttcgatc
    28141 ttatggttct tggcattggg cgatgtgtgg gcccactcga gaaatgaacc gtcgtagttc
    28201 ttcaagctcg gccatcccat cagatgaccg aaaaacagtc ccagcgtcga tctccagccc
    28261 gtcccgcaat agaaaatcaa ggtcctgtcg cgcgtgattt cccactcttt ccagaaccgc
    28321 gcaacctcga ggtaacttcg cagcgtcgag tcgagggagt cggagaaatg tcctcccacg
    28381 agggtcttgg gaccccaatg cccccacatg gcccccggga tgcgtcccct tgcggtcagc
    28441 gggattccga tgcttgcgta atcattgggg cctccgatgt attctctcca tgaacgaatg
    28501 tccacgatca aagccttggg atgcttgccg gccacgacct cctcaacata gtccgtggtt
    28561 gcgagatact cgggatgaag ggggaactcg ccgggtcccg tgccgaaatc atccaccgcg
    28621 ttcctcgctt cgggcttttc ggtcacatcg aacccgctgt ccatccagga cgtgatcccg
    28681 ccgttgagaa tcctgagatc ggacacaccc gcgtacagca aggcccacca aacccgggtg
    28741 gtggaaatgg ggtccgcact gccgtaggcc gcatcataca ggatgacggt cgtgtctttg
    28801 tcgatgccca tcttttcgat cttcttcttg agtttcttgt ccggccacag attgccgctt
    28861 ccctccacca ggccggcttc cgcggacggc cattcgacct cctccgtgcg gatatagatg
    28921 gatcccggga tcaatcgacg agtgtcgaca ctaccactgg aggttgtggc ggcaagaaca
    28981 tagcgccctg cagggggatt tggaacgggt ttgccgtcca agaggtcttt gacccactgt
    29041 gcatagacaa gcttgctgta attgcgagcc ttctccatgg gaagcgaacg gtctgccgcc
    29101 cactccaata tggaggcatc gtaattggat gccttggaat accccatcag tcgatagagg
    29161 aagtaaacaa aggcactgcg aataccacag gtgcaatagg cggtgacttc cttttttctg
    29221 gtaattcctc gggactcaaa catttccttc agggtttcgt acttcctaac ggtcttgttg
    29281 tcgttgaaga accatccgta gggaatctgt acggcccccg ggatgtggcc gcctcgagct
    29341 tccccataga gttgccagcc gatgaattcc tcatccgtcc gtgaatcaac gacaacgaag
    29401 ttcttggtgt tgagcttctt gctgatgcgc tctttggtca ttcttcggga ggcgatcacg
    29461 gaggcttcga atgtggctgc gggaagaatg ttgatcgttg cctcctcggg tcttccgtcg
    29521 gcagcccact tgtcccatcc gccgtccaag atgtgcacgt cggtacagcc caggtattcc
    29581 agcatccaga aaatgcgtcc ggcagccccg aaggaagccg aggtgttgtc atagatgaca
    29641 aaggtcatgg ttcttgtgag gcccgcctct cccaacttcc tcctcaggtc cgacttggga
    29701 agcagcccga cgccgggggt aaagaagtct gcgaacttga gattgatggc gcccggtatg
    29761 tgtgacgtgt catacccgct ggtgcgtgca tcaatgatga cgagattgtc ttccccaaga
    29821 ctatcttgaa ccgactgtgc cgaaaccagc agctttgcat tggggaattt aggctgtgag
    29881 gctttcaact ttgcgtgggc gaggtgggga ctgatcagca caagaaccag aattgccaag
    29941 aatacaggta cagttttcct tttcatcgcg ctttctcctt cattgggttg agatgggaca
    30001 atttcggaaa gtttgtgcta ccgaaagaag gaacggatgt tgctcatatc tcttgccgga
    30061 aagccttcaa cccagggggc aaacctatcc cccgcaaaga ataaagactc cttgccccga
    30121 caggccaagt cgctgcaatg tggtcgaccg tgcggcagtt tctgggtgct ggtgcgtgtt
    30181 ccgcgatccg cttttgacga cagtcgtgaa taaggaaccg tgtttcagcg gcttacgcag
    30241 atgtcttctt ttcccatcca taggacatcc aggatgggaa cttgccatga tgctatgaac
    30301 caacagccga tggctgaaca cagacctggg atggaaccgg gggacgggtt cgcactcgat
    30361 gaggacgatt cccccccagg gcatcgctga attgagccgc aacctaacgc cgttggccga
    30421 gaaatgtcca attgatttta ggcatgtttt ctcttgcatg cataggtgga ttcgatgaag
    30481 aggcacacaa atgacaagga gtcaggtctc cttatgtctc cttgagacgt ttcaatccac
    30541 gtccccgcaa gggggagcga cgggattcga gattttccgg cggccgtttg attttgttgg
    30601 aaggcttctt gaaatcccgg gcaagctttt ccgaacagga aacagcggct tgagaatcgc
    30661 cccatatcgt gatttgggtc gcgggatctg ccccgtctat ccccctcacg agagggggga
    30721 ttcgttatgt tggatctcta ctacccgaat ccggtccttc ccggtgcccc aaagcccgaa
    30781 acgacaaaga acgcgcacga ggcaggcggt ggggatgtgc gcccttccta tcgcgccttt
    30841 gtcttcctgc cggaccgttt ggcagagtcc ttttgtgctg ggttggaatg gtgggcggac
    30901 tggggtccat cgccggggaa taccccctgc ctatg
//
LOCUS       DAOOVC010000035        30564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1015_length_30564_cov_6.174571, whole genome shotgun sequence.
ACCESSION   DAOOVC010000035 DAOOVC010000000
VERSION     DAOOVC010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 30564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30564
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1015_length_30564_cov_6.174571"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLM79_06740"
     CDS             complement(<1..295)
                     /locus_tag="PLM79_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86042.1"
                     /translation="MTQPLPKDRTRSRSLYVHTLGCQMNEYDSLRLRRILGAEGYTST
                     SRMEEADVIFLNTCSVREKPEQKVYSFLGRLRRLKTRNPALKILVGGCMAQQLG"
     gene            complement(292..1053)
                     /locus_tag="PLM79_06745"
     CDS             complement(292..1053)
                     /locus_tag="PLM79_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOV86043.1"
                     /translation="MEANDQGPANPAQAIDIHVFEHGDEILREGEVSTCFYVILGGRV
                     SISRRGRTIRILRAQDVFGLEAVVLKRPSSHTARSLETSRIATYRREALDHFARQNPH
                     MIHTILASVLRQLNQTGRNLASESDSIDLGQSRVRFFGDGDEVIREGASGKEFYRLVS
                     TQGGLRIRSGDREVGHIHKPGEFLGEIAALTERPYPYTVTSIGESALETYTREDLDGI
                     VRDHPDQASHLIRSLLNHVTKKKPKPRKKPVAGKP"
     gene            complement(1301..1534)
                     /locus_tag="PLM79_06750"
     CDS             complement(1301..1534)
                     /locus_tag="PLM79_06750"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HOV86044.1"
                     /translation="MDGIPTETRVRNLLIAPREIHFLRFILEAYPGIAVVSTIDPQLG
                     LVRLNIAPGCEEEVDAVLEAEKERLQLRSVRSL"
     gene            1632..3143
                     /locus_tag="PLM79_06755"
     CDS             1632..3143
                     /locus_tag="PLM79_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86045.1"
                     /translation="MEMYPRVHGKALVCALFLCMLTTPSLTTAVEPPPQGKTAVFFSQ
                     GQSIYHPQDRARSRQEAVQDFLLQGVTQALGSFLSPSQMGSQFGLIQDKVLRQPDRYV
                     VHHQIFSESPAGGLYRVTGQVTVAMDSLKKDVVQMGLGSPEPGQRPGGAAPSGGLSRS
                     EAESASTGSLRDSGPQATRGITPSRRELYWAVAESWDQGWSVPRGTRDPRGIFAASIL
                     QESQDYPWTVRLPESGALTPDEGGNISIHPVLAQAKGLGIEKAVVGTVALRKSPNQNP
                     RIETVLRVLDVPSGKLQGEIRKQRSLRGGSTQESVLELASLIMPQLDQFMGEGGRRAA
                     PADRRFLEPQSVPPAAVAPPTRPEPPELPPAMTPPVRREVPEETPAMTPPVRSEPIES
                     PSKPAAAGPSPAPQQPPPAIRGTGEVTLSLRNLGHFAQWEELEKHIREKAKSARVTGV
                     TMGPESAQVRLEGVDGQFLESLSGTRLKGGLTVQVEGYAPDTRTVTISFGHSE"
     gene            3161..3712
                     /locus_tag="PLM79_06760"
     CDS             3161..3712
                     /locus_tag="PLM79_06760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOV86046.1"
                     /translation="MSIPNLLTICRILLTPLLVWLLLEKKLKAALVVFFIAGLTDGLD
                     GLIARVFSQKTRLGAYLDPLADKLLLVSTFILLGIQDLIPDWLAVIAVSRDILIILGV
                     STLMIHHVPLEIRPSVLSKATTLFQLLTLFAVMGSWLFPLPSPAYSVLFVVTALLSVL
                     SGIHYVHLGISLFETHRNRTDAP"
     gene            3812..4156
                     /locus_tag="PLM79_06765"
     CDS             3812..4156
                     /locus_tag="PLM79_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOV86047.1"
                     /translation="MKRFLKVMKAAGDPNRVKILKALQKRSLCVCELQAALGISQPTV
                     SSHLKVLEDAGLVAFRKSGLWVNYTLSDGEQNPYAATLLGNLRHWLQDDPEIVDLLER
                     LPTIRREEICRK"
     gene            4179..4685
                     /locus_tag="PLM79_06770"
     CDS             4179..4685
                     /locus_tag="PLM79_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV86048.1"
                     /translation="MSDEDIARIRVGRFDVSIVGLKAVMEELAESHAEEGDEKVASLM
                     IERLKGRNYIPEKARDDYGRAFVREFRRFLGRPLPEDSPGELDIKVLGMGCNQCESLA
                     QLLMQILTELRIPANLEHVRDVRQIARYGVLGSPALIINGKVVAVGSVPPRDKIRSWI
                     LEAGGTRK"
     gene            4693..5802
                     /locus_tag="PLM79_06775"
     CDS             4693..5802
                     /locus_tag="PLM79_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOV86049.1"
                     /translation="MSASNLDPKACCCPPAEPMTEDPAEARGPRSRLLRYLGMGPPLL
                     VAWILFYVSLESLAKFLTYRVLGLPAHEHLSHAVEFFVFETPKVVLLLTLVVFGVGIV
                     RSFLTPERTCRILAGKRESAGNVLAALLGVVTPFCTCSAVPLFIGFVTSGVPLGVTFS
                     FLISAPMVNEIAVVLLLGLFGWKVAVVYMGTGLLVAVTAGWVIGRLSMERHLEEWVLE
                     LRTSPACVAEDARTWADRIEYGGQAVREIVGKVWVYVVLGIAVGAGIHGYVPEGLLAS
                     FMGKSAWWSLPLAVLLGVPMYSNAAGIIPVVHALLEKGAALGTALAFMMAVIGLSLPE
                     AVILRKVLKPRLIGVFFGAVAAGILVVGAVFNFLL"
     gene            5884..5958
                     /locus_tag="PLM79_06780"
     tRNA            5884..5958
                     /locus_tag="PLM79_06780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5916..5918,aa:Val,seq:gac)
     gene            6186..8126
                     /gene="thrS"
                     /locus_tag="PLM79_06785"
     CDS             6186..8126
                     /gene="thrS"
                     /locus_tag="PLM79_06785"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOV86050.1"
                     /translation="MNSESIEVTLGDGSKKVFPRGVLALEILKAAGGSADAWIAARVD
                     GRLVDLNRRLEKNAAVEGVPSHSQEGLEILRHSAAHVMAQAVKELFPWAKVAIGPAIE
                     HGFYYDFDVENPFTAEDLERIEAKMAEIVSSRLEFQRTEMPRQEAVRLFRELGEPYKI
                     DLLESFQDDTVSVYRQGDFVDLCRGPHIPHSGFLKAYKLTGVAGAYWRGDERNRMLQR
                     IYGTAFPDREGLKQYLRFLEEAQRRDHRRLGKELDLFSLSDEVGAGMVIYHPKGALLR
                     QILETFEKREHLRRGYHIVMGPTLLKTELWKRSGHFDHYRENMYFTEIDEQSYGIKPM
                     NCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKSGVLHGLLRVRQFTQDDAHILCTP
                     QQLHGEIQSIIDFVTEVMGIFGFSHEMEISTRPEKSIGTDEDWERATAALMSALEDKG
                     IPYQVCRGEGAFYGPKIDVKLKDALDRRWQCATIQCDFTLPERFDLTYIGQDGGRHRP
                     VMVHRVILGAVERFLGVLIEHYAGAFPTWLAPVQAVLVTVTDAQQEYAGQVREKLLRM
                     GVRVELDDRNEKLGYKIREAQLQKVPYMLVIGDREVAAGLIGPRRRDGTQLEAMSPEE
                     FGSLIEKECLEASKGRVGLGAI"
     gene            8207..8713
                     /gene="infC"
                     /locus_tag="PLM79_06790"
     CDS             8207..8713
                     /gene="infC"
                     /locus_tag="PLM79_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOV86051.1"
                     /translation="MEKQVNVNEKIRASEVRVIGHDGQQLGILAIRRALELAALEELD
                     LVEVAPNADPPVCKIMDYGKYKYQQNKRFQEAKKKQTVIQVKEVKIRPKTDEHDLQVK
                     IKHVRRFLAQKDKAKITILFRGREISYSDQGTKVLERIREELKDEAVVEQPAKMEGRN
                     MVMILAPK"
     gene            8988..9185
                     /gene="rpmI"
                     /locus_tag="PLM79_06795"
     CDS             8988..9185
                     /gene="rpmI"
                     /locus_tag="PLM79_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOV86052.1"
                     /translation="MPKLKTNRAAAKRFSRTGTGKFMRARGNKSHILTKKPSKRTRRL
                     RQTTVVDAANVRALKRMLPYL"
     gene            9239..9592
                     /gene="rplT"
                     /locus_tag="PLM79_06800"
     CDS             9239..9592
                     /gene="rplT"
                     /locus_tag="PLM79_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOV86053.1"
                     /translation="MPRVKKAVKSRERRKRILAMAKGYRGGRGRLFRTATETVDRALK
                     FAYRDRKQRKRDFRSLWIIRINAAVREHGISYSRFINGLKKANLTFDRKVLAGLAVND
                     PAAFSKIVEIVKEAV"
     gene            9595..10605
                     /gene="pheS"
                     /locus_tag="PLM79_06805"
     CDS             9595..10605
                     /gene="pheS"
                     /locus_tag="PLM79_06805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOV86054.1"
                     /translation="MQEAIRKLLEDASREISGSREDPRQLDQIRIRYLGRKGELAQLF
                     KQLAQVPEALRRDCGKLLNEAKAQIEELLSLKLIEAKDRSAERRDRVDVTAPGRRPLR
                     GRLHPITQTAMEICRIFHWLGFETVEGPEVELDYYNFEALNIPKDHPARDMQDTFYIS
                     EDVVLRTHTSPLQVRVMEKRTPPIRVIAPGRTYRCDSDVTHTPMFHQVEGLMVGTNIS
                     FGDLKGLLTIFVHQMFGPETGLRFRPSFFPFTEPSAEVDIECVMCRGKGCRVCSQTGW
                     LEILGSGMVDPEVFKMVGYDPEQVTGFAFGMGIERIAMLKFGIDDLRLFFENDLRFLK
                     QF"
     gene            10838..13243
                     /gene="pheT"
                     /locus_tag="PLM79_06810"
     CDS             10838..13243
                     /gene="pheT"
                     /locus_tag="PLM79_06810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOV86055.1"
                     /translation="MNVSMNWLRNYVDIPLSVEELAHRLTMVGLEVEGVRDLGQPYRH
                     VITAKIEAILPHPRADRLRLCDLSAGRESFRVVCGAPNIQVGKVVPLALEGARLPSGT
                     EIRAATIRGETSRGMLCSQKELGLGDDASGVWILPSETPAGVPFGEALGLDDTVLEVG
                     VTPNRCDCLSMVGIAREVAAICGTRLRYPPSTLEETGPPVDRLGVVTIEDPEGCPRYA
                     ARILEGVAVGPSPSWLRRSLESVGLRSINNLVDVTNYVLMELGQPLHAFDLDRLAQNR
                     IVVRRANLGEKFITLDGVERTLFEDTLLICDGREPVAVAGIMGGLYSEISPQSTRVLI
                     ESAYFQPRSIRRSSRKLGLRTESSYRFERGIDPEGVLRAVDRAARLMMELGGGQIARG
                     RIDVYPNPYRPPVIELRVDKVNAFLGTKLDASRMAQALKSIELEVEPLDKNRLKVVPP
                     SFRPDLTREEDLAEEVARLNGYDQVPVTMPWVATQSAPLDPHMRLRHETKALLQGCGF
                     LEVLNYSFISRDWLRKLRLPPDDPRLDPIAVLNPLSEDQGVMRTSLIPGLIETARHNF
                     DHRNDDLRIFELSKVFLKREGEVLADEPHRLAGLMAGKRVPGALYGSAESVDFADVKG
                     VVEEVLELFRIPGVRYAARDLPPYLDPYRSATLFCGSDGIGSLGRLREEVREAFDLRN
                     PAFVFDLDFDRLYSLLGPPSRFRALPKFPSVARDMALVVDEELPALEIQDFLLQQQEP
                     LMEQVEIFDIYRHPQMGPGKKSLGYRVVYRAADRSLTDEEVNAIHTRLAEKVLRQFQA
                     TLR"
     gene            13253..13642
                     /locus_tag="PLM79_06815"
     CDS             13253..13642
                     /locus_tag="PLM79_06815"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV86056.1"
                     /translation="MSEKENPGLKGSDKLPQAKAPCEVIYTWGNKSYVGTSTHFHEKG
                     MLIFCRQPAPLNERVKVTLRFPDFKNVLEMEAEVVWTNIHGPSDTFRPRAMGVKFLNV
                     ERDAERLLAELASQYEAHGSIYSCYYT"
     gene            13783..14334
                     /locus_tag="PLM79_06820"
     CDS             13783..14334
                     /locus_tag="PLM79_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOV86057.1"
                     /translation="MKFLEIFLVATALGCDAFAVGMGVGTKFCAPRQVFRLSFHFGLF
                     QFLMPLLGWFAGQNLVGIARQWGPWIGFLLLFFIGAKMFYESFGGPEEERKEECRDPT
                     RGFSLVMLSVATSMDALGVGFSLGILGQSLLTAAVWIGITACLMTWTAMKLGNRLSEK
                     FGKRMETVGGVILMAIAVKLVTL"
     gene            14352..17342
                     /locus_tag="PLM79_06825"
     CDS             14352..17342
                     /locus_tag="PLM79_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOV86058.1"
                     /translation="MAVKLEIALKEDLFDAEGAGLCRKILDYFGWKVGTARVVHILTL
                     DTALSREELEAVRKEIFTNPVTQCSGFHSLAGEFDWAIWVGYRPGVRDTAGSVAMEAM
                     ADYLGRSFEPWEAAYTSKLYLLGNASLDQSRVEVIARELLANEIIQQWRVYSRESRDR
                     DQGIGIIVPKVVLDHEPSVRTLPIPSDEELMRLSRERNLALNPQDVPVIRSYFTRPHV
                     MEARIRAGLALPTDVELEAISQARSDHCNHNTFRGKFHYRDLGTGESCVVDNLFKSCI
                     EAPTLEIRQKKDWVISVLWDNAGVARFDENHHYAVTGETHNSPSNMEAYGGSLTGIVG
                     VYRDIMGTGKGARLVAGLYGYCVAPRDHAGPLKPHLHPRRLLDGIVEGVRDGGNKHGV
                     PTASGILFFHPSYLGKCLVFVACLGIMPREVHGQPTAEKCPQPGDWILMSGGRVGKDG
                     IHGVTASSESYSEHTPAGHVQIGDPYTQKKMHDFLLEARDRGYITFITDNGGGGLSSS
                     IGESARLAGGASVELEKVPLKYAGLDVWEIWVSESQERMTVAVSPEHLDRFLELSRKH
                     EVESTVIGRYENSGKLHLTYHGRTCAYLDLSLFSEDFPQWEFEAEWLPPESRGLWEPV
                     LGEPRQYGTLLRTLLGRPNLCSREWIQRQYDHEVQGAAAVKFLVGRRRDIPNDAVVYR
                     PVLEGRKGLVMAQALLPTLSLIDTYDMVAATMDEAVRRILSVGGGLEEIGGVDNFCWP
                     NIQYDPVTNPDGRYKAAQLVRANWALRDVCLAYGIPLLSGKDSMYVDGHLEGAFGERH
                     KVSGLPTLQFTATATMPDVHRALSMEPKMPGDLVYILGITRDELGASEYYDLFGLVGR
                     NAPRLDPRTVLPLYRAVEHCIARGLLASCHGIYRGGLGIHASMMALGAELGLDLDLSR
                     VPADPAALRDDRVLFSESCGRFLVTVSPSQQEDFEEQFRNLPCGLLGRVIEELVLRVR
                     GRSGDVLLEEPLAELRRAWMASNG"
     gene            17359..18168
                     /locus_tag="PLM79_06830"
     CDS             17359..18168
                     /locus_tag="PLM79_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOV86059.1"
                     /translation="MQPVRALVLTGFGLNCDVETAYALEMAGARTERVHLNGLILGER
                     RLEEFQILVIGGGFSWGDDHGAGVIMAMRIKHRMKDDLLDFVDKGGIVVGICNGFQVL
                     VNLGLLPGFEPRRLKREVALIHNDCGNFLDQWVHLAANPSSPCVLSRGIQRLELPVRH
                     GEGKFFAPPAVLDRLADQKQVAFTYIRPDGRPADGEAPHNPNGSLGDIAGICDPTGRI
                     TGLMPHPEAFNHWTNHPDWTLHKEIARRIGSSLPEEGEGVRIFRNAVEYFR"
     gene            18390..19220
                     /gene="purN"
                     /locus_tag="PLM79_06835"
     CDS             18390..19220
                     /gene="purN"
                     /locus_tag="PLM79_06835"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOV86060.1"
                     /translation="MASNRKNLRLAILISGGGTNLQAILDRCAAGLLPAKVVVVAADR
                     PDAYGLTRARRAGVPSHVVDYSRYLSGAMDSRISPELEVLAESLDSRQRILRESDRPK
                     RLRRLVRLLLAENEMIRVIESGQPDYLCLAGFMRLVTPHFLAHFHGARPWRVLNIHPA
                     LLPAFPGRRGYEDTFDYGCKWGGVTVHFVDEGEDTGPILAQAVYPIWPDEDLEAVRSR
                     GLELEYELYSQCIRWLWDERVEVRRREDGRWFTVIHDPSYPRILKEWVDRAMSSASGS
                     "
     gene            complement(19352..20743)
                     /gene="cbpB"
                     /locus_tag="PLM79_06840"
     CDS             complement(19352..20743)
                     /gene="cbpB"
                     /locus_tag="PLM79_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-modifying radical SAM enzyme CbpB"
                     /protein_id="HOV86061.1"
                     /translation="MNPAREYEHRPLYANTGYGPNFSVLEIGLPDQVAVVEADRAFWA
                     VVRREALEEALAGGLVDQFRKAAGSLSREMHQLRFGLKPSAVYFNPTERCNFNCSYCY
                     LPEEMRRNGTTMTAAQLSRALGILRDYFSSVLPPGVKPQLIFHGSEPLLAREAVFAGI
                     DEYRDDFVFGIQSNATLLDDEAITFLEDRGVGIGISLDAPTADLADRTRKNWKGAGAF
                     ANVVSVIEKLSPYPAFNVITTVTRVNVESLPAMVDFYHGHGVRTVLFNPVRVTRQGGL
                     DLKPEDSVLARHFFRALDRAEELYRETGRKLVTANFANILAAILGPTSRRLMCDITPC
                     GGGRCFFAVSARGEVFPCSEFIGFPRFNGGNLFKNSLSSILGGSPFRDVTSRTAERIV
                     PCGNCAIRHFCGAPCPAEVHAVSGTLNAPSPYCAFYEEQVRYAFRVIARGREEAYLWD
                     GWEEETEETFCWK"
     regulatory      complement(20988..21125)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            21442..21939
                     /locus_tag="PLM79_06845"
     CDS             21442..21939
                     /locus_tag="PLM79_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOV86062.1"
                     /translation="MAQKKKVVENAGKEKADPTSCETKSCNFICSRDTLDGAYPALIL
                     AVNARRLGMEAGIFYTFMGVRLILKDGIRKAQYIPPGFMGAIPGMAAVATKMMRKQID
                     EAGIPSLEELLEMAQLEGVKLVACKMTIDMMKLKEEDFIEGVEIQTAEDFLKHAVTCQ
                     INLFT"
     gene            complement(22205..22390)
                     /locus_tag="PLM79_06850"
     CDS             complement(22205..22390)
                     /locus_tag="PLM79_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86063.1"
                     /translation="MEECKECELAVYCYSDPSAWVFRTKQEMDEKQAAIRQCPVSEKA
                     QNNRTLSPSASGQVRAR"
     gene            complement(22393..22749)
                     /locus_tag="PLM79_06855"
     CDS             complement(22393..22749)
                     /locus_tag="PLM79_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOV86064.1"
                     /translation="MTTGSGNPYFEVSASPEALRREKEKAKALRKTQWWQRQLAKGTC
                     HYCGKRVPSLELTMDHVVPLVRGGGSTRGNVVPSCKQCNSRKKYLLPVEWDEYLQGLS
                     RGTDDPSHPDRKPKEP"
     gene            complement(22766..24211)
                     /locus_tag="PLM79_06860"
     CDS             complement(22766..24211)
                     /locus_tag="PLM79_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOV86065.1"
                     /translation="MKVNKVLAVAGSVCCLLLASLAFVGWSSPDVQAALPSFGAQGPP
                     SFADLSEHVKHAVVNISTTQVLKENPMQPFFSPHSPFREFFGDEFFKRFFGDTPHGEM
                     KTQALGSGLIMDEEGLILTNNHVVEKADEIKIKIESGKEYDAKVVGRDPKTDLALIRV
                     TPDSAFPKPARLGNSDNIRVGDWVMAVGNPFGLGHTVTAGIISAKGRVIGAGPYDDFL
                     QTDAAINPGNSGGPLFNMNGEVVGINTAIVAQGQGIGFAIPINVAKDILAQLKSGKVI
                     RGWLGVMIQDITPELAQSFGIKETKGVIVADVVADGPADKAELKRGDVVVKFNGKEVE
                     NSPSLSRMVAATAPNTAVTISVLRDGKPREIKVTIGTMPDEVAEPPAVKKESAWGLTV
                     QNLTPELAQRFGWSEGEKGVVISGIQPGSPGAEAKLRTGDLIKEVNRHKIQNLRDYKQ
                     ALEKTKKGESLLLLVKRGENTFYVALKPPKS"
     gene            24507..24842
                     /locus_tag="PLM79_06865"
     CDS             24507..24842
                     /locus_tag="PLM79_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86066.1"
                     /translation="MERDRLMLTEACDQAIQLVSQTREMERLYQKAVQSVGEGALRTG
                     ILGLAVDAMEDEELKNEVFVNDESLLSFFCGIWIQYLLVEVAGITREKLKALAADALR
                     QLQKDKRVH"
     gene            complement(25179..27398)
                     /locus_tag="PLM79_06870"
     CDS             complement(25179..27398)
                     /locus_tag="PLM79_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV86067.1"
                     /translation="MDSQATERKGRPALPAPVSGEPHFFDMSLYHARMAKYLDPCREE
                     NAVFFAALEFARKLHTGQRRRSGAPYISHPCAVAEILAREFHFRDAHLLASALLHDVV
                     EDVPSITIEDIEREFGPTVAELVDGCTKLTRSHLDRPTLRDLTHSKIFLSASRRLGVL
                     IIKLADRLHNLRTLHYLPQTKRQRIAHETLEVYAPIAARFNLFSLKRELYHLALSFLY
                     PRKSRKILQYLRELRGSTDLLNIENTLQRTFSAPPSPPAAVRMRTKGLGSYYNPLKRT
                     LDLGNTENHVDFVLVLQTDNVLDCYCALGRVNTTFTPIPRTVRDFIANPKNNGYRSLH
                     VRIHVGGQNYLIKIRTSEMDHWSSLGILHEWDTEKALSDEHWQEISELLRSIGEYGGA
                     GPQRKALIRLSEAEEIFVYSPDGDIYYLPKRSVVLDFAYRIHSELGDYCQGALINGVW
                     VPSTHPLKDGDVVEIVVSSDPLDPDLQLESVCKTPKARAAVNRRLQQKRRRYAENAGR
                     QILHQELERHGFPPDALDEEKLRLVSEVLNLKGSTEIYTKIGQDLLSPHLVLYYEEGS
                     RESDSGGETNVLRVEELDKAVHKFARCCNPFPGQKDVMATLSERGITFHHKSCSDLVQ
                     RHGLQPQQLVEVEWDARNPWPHPLIFHLVVFQEAPMSLLQSLTSLPGSFDIRLVESLV
                     DKHDLPCVRMSVQLGDFAEARSLFALLPEKRVAIEDYERGEPPRSIRTSTESPAGWR"
     gene            27792..29828
                     /gene="fdhF"
                     /locus_tag="PLM79_06875"
     CDS             27792..29828
                     /gene="fdhF"
                     /locus_tag="PLM79_06875"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:28197..28199,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOV86068.1"
                     /translation="MNYESVLTTCTYCGCGCGMYLQVLDGRILNTLPSKTSPVNEGKL
                     CVKGWYIHEFVQHPTRLTKPLLREDGSLRETSWDTALDYAASELKRIRDAHGPDSIAF
                     LTSAKCTNEDNYLMQKFARAVIGTNNVDHCARLUHSSTVAGLAASFGSGAMTNSFAEL
                     EDADCIFIIGSNTTVGHPLVATRIFRAKSKGAKLIVADPRQTQIGRLADLYVRQRLGS
                     DVALINGMMHVILKNGWQNQAFVESRTENFEAFAQVIEKFPPERAAEITGVSADDIVR
                     MAEYYAKAEAGSIVYCMGITQHTTGVDNVRSLANLSMLCGHIGRPSTGVNPLRGQNNV
                     QGACDMGGLPNVYPAYQAVTVPEVREKFEKAWNVKLPSNVGLTIMEMMDGIQHGKIKG
                     LVIMGENPMVSDPDIHHVKHALEAAECLVVLDIFPTPTTELAHVVLPAASFAEKDGTF
                     SNSERRVQLVRKAVEPPGEAKADWEIIRELSNRLGYPMDYKSSEEIFEEIRKVAPSYA
                     GMTYPRLEGEGLCWPCPTTEHPGTAYLHAGKFSRGLGLFAAIDYRPPAEEPDEEYPFW
                     FTTGRAYVHYHTGTMTRRSSHLDQEMPESVTEVHPEDAKRMSLREGDVVEVASRRGSV
                     FSRVQITDRIGKGVVFMPFHFAESAANTLTNAALDPVAKIPEYKVCAVKLAKVA"
     gene            complement(29806..30303)
                     /gene="rnhA"
                     /locus_tag="PLM79_06880"
     CDS             complement(29806..30303)
                     /gene="rnhA"
                     /locus_tag="PLM79_06880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOV86069.1"
                     /translation="MPEAPAHGIMTLQPRKKVEIFTDGACSGNPGPGGWGAILRYRGV
                     EKEISGYSPHTTNNRMEMTAVIEALRALKEPCRVVLYTDSRYLRDGITRWIGRWKQNG
                     WKTSEKTPVKNKDLWEALEALTQRHEIHWNWIKGHAGHLENERCDRLAREAMEKGPVK
                     PPSQA"
     gene            complement(30296..>30564)
                     /locus_tag="PLM79_06885"
     CDS             complement(30296..>30564)
                     /locus_tag="PLM79_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86070.1"
                     /translation="FFLKVHDISWSITSGIVQDNLVVVVRNDGYRKDAGKIVRRAFES
                     LGSAGGHQAMARAEIPLKELTQVLEKRTSTAIERFIRRRLSPFA"
BASE COUNT         6025 a         9109 c         9344 g         6086 t
ORIGIN      
        1 ttcccaactg ctgggccatg caaccgccca ccaggatctt gagggcgggg ttccgtgtct
       61 taaggcggcg cagcctgccc aagaaggagt acaccttctg ttccggtttc tcccggaccg
      121 agcaggtgtt caagaagatc acgtccgcct cctccatacg ggaagtgctc gtataccctt
      181 cggcccccag gattcgccgc aaacgcagcg aatcgtattc attcatctga cagccgagcg
      241 tgtggacgta gagggatcga gatcgtgtgc gatccttggg aagcggctgg gtcatggttt
      301 ccctgcgaca ggcttcttcc tgggctttgg cttctttttc gtgacatgat tcaagaggga
      361 tcggatcaag tgggaggcct gatcgggatg gtcccgcacg atcccgtcca ggtcctcccg
      421 ggtgtaggtc tcgagggcac tctccccgat actcgttacg gtgtacgggt aagggcgctc
      481 cgtcagcgcg gcaatctccc ccaggaactc ccccggcttg tgaatgtgcc cgacctcgcg
      541 gtctcccgac cgaatcctca atccgccctg ggtggaaacc aggcggtaga attcctttcc
      601 cgaagcgccc tcgcggatga cctcgtcccc atccccgaag aaccggacac gggattggcc
      661 gaggtcaatg gaatccgatt cgctcgccag gttgcgaccc gtctggttga gttgccgcaa
      721 caccgacgcc agaatggtgt ggatcatgtg ggggttttga cgggcgaaat ggtccagggc
      781 ctccctccgg tacgtggcga ttcgagaggt ctccagggac cttgccgtgt ggctggaagg
      841 ccttttcaac acgacggcct cgagcccgaa cacgtcctgg gcccgaagga tccgtatggt
      901 ccttccccgc cgactgatgg acactctgcc gcccaggatc acataaaagc acgtggaaac
      961 ctctccctcc ctgaggatct catccccgtg ctcgaataca tgaatatcga tcgcctgcgc
     1021 cggattcgca ggcccctggt cgttcgcttc cacggggact tccttgtgca ttggggtggt
     1081 gaaagggcgc tttctcgaga ccggcacggc ggagcatgga tcattctcag gagaaagcat
     1141 gacggttcaa ttggctaacc ccttatttac atgagtaaat tcagccttcg aatccttatt
     1201 agccaatttc cccctccttt gcaagcaaat aacaggaaca gagcggcccg gcaaccgttt
     1261 gccgggtttc acgagggggt gaagaaggaa ggacccggaa ctacagggac cggaccgatc
     1321 tcagttggag gcgctccttc tcggcttcca ggacggcgtc cacctcctcc tcgcatccgg
     1381 gggcgatgtt gagacgcacc aggcccagct gagggtcaat agttgagacc acggcgattc
     1441 ccgggtaggc ctccaggatg aatcgaagaa agtgaatctc ccggggggcg atgagaaggt
     1501 ttcggacccg ggtttccgta gggatgccgt ccacggattt ctccttttgt ttccaacggg
     1561 tctatgatac caaatacccc tcggtgtagg gaagttgttc gtgtccgtcg agtcccagag
     1621 gaatcatgtc tatggaaatg tatccgcggg tgcatgggaa agcccttgtt tgtgccttgt
     1681 ttctatgcat gctgacgacc ccttcgttga cgaccgctgt ggaaccgcct ccccagggga
     1741 aaaccgccgt cttcttcagc cagggacagt ccatctacca tccccaggat cgtgccagaa
     1801 gccgtcagga ggccgtccag gacttcctcc tccagggggt gacccaggcc ttggggagtt
     1861 tcctgtcccc atcccagatg ggaagtcagt tcggactcat tcaggacaag gtgcttcgac
     1921 agcccgatcg ctacgtcgtc caccaccaga tcttttccga atcgcccgca ggcggactct
     1981 accgcgtcac ggggcaggtg accgtagcca tggacagtct caagaaagac gtggtgcaaa
     2041 tgggtctcgg atctcccgaa cccgggcaac gccccggggg tgcggcgccc tcgggcggac
     2101 tctcccgttc cgaggcggaa tccgcgagca ccggctcgct tcgggattcg ggaccgcagg
     2161 cgacccgggg gatcacccct tcccggcggg agctctactg ggccgtggct gaaagctggg
     2221 accaaggatg gtccgtgccc cggggaaccc gggatcctcg aggcatcttt gcggcctcga
     2281 tcctgcaaga atctcaggac tacccatgga ctgtgcgatt gcccgaatcg ggggcgctga
     2341 ccccggatga gggaggcaac atttccatcc accccgtcct ggcccaggcc aaaggactgg
     2401 gaatcgagaa ggcggtggtg ggaaccgtgg cccttcgtaa gagccccaac cagaaccccc
     2461 gcatcgagac ggtgctgcgg gtgctggacg ttccgtcggg aaagctgcag ggggaaatcc
     2521 ggaaacaacg gtccctccgg ggcggctcca cccaggaaag cgtcctggaa ctggcttccc
     2581 tcatcatgcc ccagttggac caattcatgg gcgaaggggg gagaagagcg gccccggcgg
     2641 atcgtcgatt cctcgaacct caaagtgtac cgcctgcggc ggtggctcct cccacccggc
     2701 ccgaaccgcc cgagctcccg cccgccatga ccccaccggt tcgaagggag gtccccgaag
     2761 agacgcccgc catgacccca ccggttcgat ccgaacccat tgaaagcccg tccaaaccgg
     2821 ccgccgccgg tccgtctccc gctccccaac agcccccgcc cgccattcgc ggaaccggag
     2881 aggtcactct ttccctgcgc aaccttggtc atttcgccca gtgggaagaa ctggaaaagc
     2941 acattcgaga gaaggccaag tctgcccggg tcaccggggt gaccatgggt cccgagagtg
     3001 cccaggtccg attggagggg gtggacggtc agtttctcga gtccttgagc gggacccgcc
     3061 tcaagggggg cctcacggtc caggtggaag gatatgcccc cgatacccgt accgtcacga
     3121 tttccttcgg tcattccgaa tgaaaaaggg gttcggcggc atgagcattc ccaacctgtt
     3181 gaccatctgc aggattctgc tcaccccttt gctggtatgg ctgctcttgg aaaagaagct
     3241 caaggcggcc ctggtggttt tcttcatcgc cgggttgacc gacgggctgg acggcctcat
     3301 tgcccgagtc ttcagtcaga aaaccaggct gggtgcctat ctggatcccc tggcggacaa
     3361 gcttctcttg gtttccacgt tcattctcct gggaatccag gacctgatcc ccgattggct
     3421 ggcggtgatc gcggtgagcc gggacattct gatcatcctg ggagtatcga ccctcatgat
     3481 tcaccacgtt cccctggaga ttcgaccgtc ggtattgagc aaggcgacca cccttttcca
     3541 gctgctcacc ctgtttgcgg tcatggggtc ctggctgttt cctctgcctt cgccggctta
     3601 ctcggtcctt ttcgtggtta cggcccttct ttcggtcctc agcgggattc attacgttca
     3661 tttggggatt tcccttttcg agactcacag gaatcgcacg gacgctcctt aggagggctt
     3721 ctttgggagg agggagcggc tttcgggaat tttccttgac ccgcctcggt gctatctata
     3781 tatttacatt caccgatata tggggggcgc tgtgaaacga ttcctcaaag tcatgaaggc
     3841 cgccggggac cccaaccggg taaagatcct caaggccctt cagaaacgat ccctgtgtgt
     3901 ctgcgaactc caagcggctc tgggcatctc ccagccgacg gtctcgagtc acctcaaggt
     3961 cctggaagat gccgggttgg ttgccttccg caagagcggc ctgtgggtca attacacgct
     4021 gtcggacggc gaacagaacc cctatgccgc gactctactc ggcaaccttc gccactggct
     4081 ccaggatgat cccgaaatcg tcgacctgct ggaacgcctc cccaccatcc gccgggagga
     4141 aatctgtcga aaatgaagcc tttgagtcga gggaatccat gagcgacgag gacattgcac
     4201 gcatccgggt gggccgcttc gacgtgagca ttgtcggcct caaggccgtc atggaagagc
     4261 tggccgaatc ccatgccgag gaaggcgacg aaaaggttgc ttccctcatg atcgagaggc
     4321 tgaaaggacg aaattacatc ccggagaagg ctcgagacga ctatggacgg gccttcgtgc
     4381 gggagtttcg caggtttctc ggacgacctc ttccggagga ttctccgggg gaactggaca
     4441 tcaaggtcct ggggatgggc tgcaaccagt gcgaaagcct ggcccagctt ctcatgcaaa
     4501 ttttgacgga acttcggatt cccgccaacc tcgagcatgt ccgggacgtc cggcagattg
     4561 cccgttacgg ggttctgggc agcccggccc tgatcatcaa cgggaaggtc gtcgccgtag
     4621 ggtcggtgcc ccccagggac aagatccgca gctggatcct ggaagccggt ggaacccgga
     4681 aatagccatc ctatgtccgc atccaatctc gaccccaagg cctgttgctg cccccccgcc
     4741 gaaccgatga ccgaagatcc ggctgaagca aggggccctc ggtcccggct cttgcgctat
     4801 ttggggatgg gtccgcccct cctggttgcc tggattctgt tctacgtcag cctggaaagc
     4861 cttgccaaat ttctcaccta ccgggtcctg ggacttcccg cccatgagca cctgagccac
     4921 gccgtcgagt tcttcgtttt cgagactccc aaggttgtcc tgcttctgac gctggtggtc
     4981 ttcggggtgg gcatcgtacg atcctttctc actcctgagc gaacctgcag gatcttggcg
     5041 ggaaagcgcg agtccgcagg gaatgtgctc gcggcccttt tgggcgtggt gacccccttt
     5101 tgcacctgct ctgcggtacc ccttttcatc ggttttgtaa cctcgggggt cccccttgga
     5161 gtcaccttct cctttctcat ctccgctccc atggtcaatg aaatcgccgt ggtgctgctg
     5221 ctgggccttt tcggatggaa ggtggcggtt gtctacatgg gcacgggcct cctcgtagcc
     5281 gtgacggcgg ggtgggtgat cggcaggctt tccatggagc gtcatctgga agagtgggtc
     5341 ctggagcttc ggacgagtcc cgcctgcgtc gcggaggatg cccggacctg ggccgaccgc
     5401 atcgagtacg gggggcaggc ggtgcgcgaa atcgtgggga aagtgtgggt ctacgtggtc
     5461 ctgggcattg ccgtgggagc cgggatccac gggtacgtac ccgaaggcct cctggcttcc
     5521 ttcatgggca aatcggcctg gtggtctctc ccgttggcgg tcctgctggg ggtccccatg
     5581 tattccaacg ccgcgggcat catccccgta gtccatgcgc tcctggagaa gggagccgcc
     5641 ctgggaacgg ccctcgcctt catgatggcc gtcatcgggc tctccctgcc cgaggccgtc
     5701 atcctgcgaa aggtcctcaa accgcgcctc atcggcgtct tttttggagc ggtggctgcg
     5761 ggcatcctgg tggtgggcgc cgtcttcaac ttcctgctgt aggccgttcc caactccgca
     5821 ttctgccggc atttctcttg acacgaccag gcaagaccgt tatagagtca ccaaaaagcg
     5881 tgtaggcacg tagctcagtg ggagagcgct accttgacgc ggtagaagtc ggcggttcga
     5941 aaccgcccgt gcctaccagt tttgtgcccg attcggcacc cctggaaaat gaagcttgaa
     6001 tgaaaatccg ggcgagaccc tataggggcg gcactgacct gaaaaacagc aggaagaaga
     6061 gtattccaaa cggagcaaga tctcccattg aggcatcttc ccacactgtg aggcaggtgc
     6121 tttttttttc cctttcccgg ggaggggaac ccccggggat tgaacaccaa tttcagggga
     6181 atcgaatgaa ttccgaatcc atagaagtca ccttggggga cgggtccaag aaggtcttcc
     6241 ccaggggagt tttggcgctg gagatcttga aagcggccgg tgggtccgca gacgcgtgga
     6301 tcgcggcgag ggtcgacggg cgtctggtgg atctcaaccg gagactcgaa aagaacgcgg
     6361 ccgtcgaggg ggttccaagc cactcccagg aaggattgga aatcctgcgc cacagtgccg
     6421 ctcacgtcat ggctcaagcg gtcaaggagc tcttcccctg ggcgaaggtt gccatcggac
     6481 ccgccatcga gcacggtttt tactacgact tcgacgtgga gaacccgttt acggcggaag
     6541 acctcgaaag aatcgaggcc aagatggccg agatcgtgtc ttcccgtctc gagttccagc
     6601 gcacggaaat gccccggcaa gaagcggtcc gcctcttccg agagctgggc gagccttaca
     6661 agatcgacct cctggaatcc ttccaggacg acacggtatc cgtgtaccgc cagggagact
     6721 ttgtggacct gtgccgcggc ccccacatcc cccactccgg attcctcaag gcctacaagc
     6781 tgaccggggt cgccggggcc tactggcggg gagacgagcg gaaccggatg ctgcagcgaa
     6841 tttacggcac ggcctttccg gaccgggagg gtttgaagca gtatcttcgt ttcctcgagg
     6901 aagcccaacg ccgggaccat cgacgcctgg gaaaggagct ggaccttttc agcctcagcg
     6961 acgaggtcgg ggccggcatg gtgatctatc accccaaggg ggcgcttctg cgccagatcc
     7021 tggaaacctt cgagaaacgg gagcatcttc gccggggcta ccacatcgtc atgggtccta
     7081 ccttgctcaa gaccgagttg tggaagcgtt cgggccactt cgatcattac agggagaaca
     7141 tgtattttac ggaaatcgac gagcagtcct acggcatcaa gcccatgaac tgcctgtcgc
     7201 atatgctcat ttataagtcc aagatccgca gttaccggga cctgcctctc cggtacttcg
     7261 aactgggcac ggttcaccgg cacgaaaagt ccggggtgct ccacggcttg ctgcgggtcc
     7321 gccaattcac ccaggacgat gctcacattc tttgcacccc ccaacagttg cacggagaga
     7381 ttcaaagcat catcgatttc gtgacggaag tgatggggat ttttggcttc tcccatgaaa
     7441 tggaaatcag tacccggccc gaaaaatcca ttggcacgga cgaagattgg gagcgagcca
     7501 ccgcagccct catgtccgcc ctcgaagaca aggggattcc ctaccaggtt tgtcgtgggg
     7561 aaggggcctt ttacggcccc aagatcgacg tgaagctgaa ggatgctttg gaccgccgct
     7621 ggcaatgcgc caccattcaa tgcgatttca ctcttcccga gcggtttgat ctgacctaca
     7681 tcgggcaaga cggagggcgt caccgtccgg tgatggttca ccgagtgatt ttgggtgcgg
     7741 tggaacggtt tctgggggtc ctcatcgagc attacgcggg ggcgtttccc acctggcttg
     7801 cgccggtgca ggccgtcctg gtcacggtga ccgatgcgca gcaggaatat gccgggcagg
     7861 tccgagagaa gcttctccgc atgggggtgc gggtggaact cgatgaccgg aatgaaaaac
     7921 tagggtataa gatccgtgag gcccagttgc agaaggtccc ctacatgttg gtgatcggag
     7981 accgggaggt tgccgcggga ctcatcggtc cccgacggcg ggacggcacc cagctggaag
     8041 ccatgagccc ggaagaattc ggctctctca ttgaaaagga atgcctcgaa gccagcaaag
     8101 ggcgggttgg cttgggcgca atctaaggcg tggtttcccg ggacgggagc gcctgtcgga
     8161 cgggagaaag ggcgatccca ccacttttgg acaaggagga acaacactgg agaaacaagt
     8221 caatgtcaac gaaaagatcc gggcttccga ggtgagagtc atcgggcacg atggacagca
     8281 gttggggatt ctcgccatca ggagggcctt ggagctggcc gcgctggagg agctggatct
     8341 ggtggaagtg gctcccaatg ccgatccgcc ggtctgcaag atcatggatt acggcaaata
     8401 caagtaccag cagaacaagc ggtttcagga agccaagaag aaacagaccg tcattcaggt
     8461 caaagaggtc aagattcggc ccaagaccga cgagcatgat cttcaggtca aaatcaaaca
     8521 tgttcgcagg ttcctcgccc agaaagacaa ggccaagatc accattttgt ttcgaggccg
     8581 agagatcagt tactcggatc aggggacgaa ggtcctggag cggattcggg aagagttgaa
     8641 ggatgaggcg gtggtggagc agccagccaa gatggagggc cgaaacatgg tgatgatcct
     8701 ggcccccaag tgagtccggc ggagccctgg aaaaaggccc ttgacacccc tgcggaactt
     8761 caataaattc cattgcttcg tctgtgagaa ccccggggtc ggcatgactc taaaatcgcc
     8821 cggaaacggg ctggaccttc ggacggccgg gtacgcgccc ggaaaccggg gtttctccat
     8881 gaagcaagga cactcggtgc ttgctctgaa gatggattcc ttacgcgggc caagaacccc
     8941 cggagctatg ccgggatacg ctcggggggc cgtttatgga ggatggaatg ccaaagttga
     9001 aaaccaatcg cgcagcagcc aaacgctttt cgaggacggg cacgggcaag ttcatgcggg
     9061 ctcgaggcaa caagagtcac attcttacca agaagccgtc caagaggacc cggcggcttc
     9121 gccagacgac cgtggtggat gccgccaacg tccgggccct caagcggatg ctgccgtacc
     9181 tgtgagtctc aaaggatgga tgggtcccgt cagggacccg aacaaggagt gatgtctcat
     9241 gcccagggtg aagaaagccg taaaatcgcg cgaacgccgc aagaggattt tggccatggc
     9301 caaaggatac cggggaggcc ggggaaggct tttccggact gccaccgaaa ccgtggatcg
     9361 cgccctgaaa tttgcctacc gggaccgcaa gcagcgcaaa agggattttc gcagcttgtg
     9421 gatcatccgg atcaacgccg ccgttcgaga gcacggaatc agctactccc ggttcatcaa
     9481 cggcctcaag aaagccaacc tcacctttga ccgcaaagtg ctggcggggc tggccgtcaa
     9541 tgatcccgca gccttttcca agatcgttga gatcgtgaaa gaagcagtct agagatgcag
     9601 gaagccatcc ggaagttgct ggaggacgcc tcccgggaaa tctccgggtc ccgggaggac
     9661 cccaggcagc tggaccagat acggatccgt tacctgggac ggaaggggga actggcccaa
     9721 ttgttcaagc agttggccca ggttcccgaa gccctgcgac gggactgcgg aaagctcctc
     9781 aacgaggcaa aggcccaaat cgaggagctt ctgtccttga agctgatcga agccaaggat
     9841 cgcagtgccg aacgccggga tcgcgtggac gttaccgccc cgggtcgaag gccccttcgc
     9901 ggccgccttc atcccatcac ccagacggcc atggaaatct gccgaatttt ccactggctg
     9961 gggtttgaga ccgtagaggg tcccgaggtg gaactggact actataattt cgaggccctg
    10021 aacatcccca aagaccatcc ggcccgagac atgcaagaca ccttctacat ttccgaggac
    10081 gtggtactcc ggacccacac ctcgccgctc caggtccggg tcatggaaaa gcgcacccct
    10141 cccatccggg tcattgcccc gggaaggacg taccggtgcg actcggatgt gacgcacacg
    10201 cccatgtttc accaggtcga gggtctcatg gtgggaacca acatctcctt tggcgacctc
    10261 aaggggctcc tgacgatctt tgtccaccag atgttcgggc ccgagaccgg cctgcgcttt
    10321 cggcccagtt tctttccttt tacggaaccc agcgccgaag tggatatcga gtgcgtcatg
    10381 tgccgcggga agggatgcag ggtttgctcc caaacgggtt ggctcgagat cctgggctcc
    10441 ggcatggtgg accccgaagt tttcaagatg gtgggctacg atccggagca agtgacggga
    10501 ttcgcgttcg gcatggggat tgagcgcatc gccatgctca agttcgggat cgacgacctg
    10561 cgacttttct ttgaaaacga cctccgtttt ctcaagcagt tctaggaatc ggttccgctc
    10621 ggcccactcc cggaagccca cggcgggcca aatgagctcg ctccccgcag gctgcctcct
    10681 tcccttcgag acgctgggaa acgaccctgt ccccgccgtc cgggaccacg actccgtcgg
    10741 aaagcgcgga ctctttccat gtgcgggaag ggaggacggg ggccgaggca gagaggtctt
    10801 tgccaccttt cttggacttc atcattagga agcacccatg aatgtcagca tgaattggct
    10861 tcgcaactac gtggacatac ccctttccgt ggaggaattg gcccatcggc tcaccatggt
    10921 cggtctcgag gtcgaggggg tccgagacct gggacaaccc taccggcacg tgatcacggc
    10981 caaaatcgag gcgattctcc ctcatcctcg tgcggacagg ctccgcctgt gcgatctgtc
    11041 ggccgggcgt gaatccttcc gcgtggtttg cggagctccc aatatccagg tgggaaaagt
    11101 cgtcccgctg gctctcgagg gggccaggct tcccagcggc accgagatcc gggccgcgac
    11161 cattcgggga gaaacctccc gggggatgct ttgctcccag aaagaactgg ggctggggga
    11221 cgacgcgagc ggggtttgga ttcttccttc cgagactccc gcaggagttc cctttggaga
    11281 ggctttgggc ctcgacgaca cggtcctcga ggtgggagtc actcccaacc ggtgcgactg
    11341 tctgtccatg gtgggaatcg cccgggaagt ggccgccatc tgcggcacaa ggctccggta
    11401 cccgccttcc accctcgagg aaacggggcc tcccgtggat cggctgggtg tggtcaccat
    11461 cgaggacccc gaaggctgtc ctcgatatgc cgccagaatc ctcgagggag ttgccgtcgg
    11521 gccttctcct tcctggctcc gccgaagcct ggaatccgtg gggcttcgct ccatcaacaa
    11581 cctcgtggac gtcaccaatt atgtcctcat ggaactggga caacccctcc atgcctttga
    11641 cctggaccga ttggcccaaa accgcatcgt ggtgcgaaga gcgaacctcg gggagaaatt
    11701 tatcaccctg gacggggtgg aacggaccct cttcgaggac actctcctca tctgcgacgg
    11761 aagggaaccg gtggccgtcg ccggaatcat gggagggctg tactcggaaa tcagccctca
    11821 gagcacccgc gtcctcattg agagcgccta tttccagccc cgcagcattc gtagaagcag
    11881 caggaagttg ggccttcgaa cggagtccag ctaccggttc gaaaggggga tcgatcccga
    11941 gggcgtgttg cgggccgtgg accgggcggc tcgactcatg atggaactcg gaggcggtca
    12001 aatagcccgg ggacgcatcg acgtctaccc caatccctat cggccgccgg tcatcgagct
    12061 tcgagtggac aaggtcaacg cgttcctggg cacgaagctc gatgcttccc gcatggccca
    12121 ggccctcaag agcatcgaac tcgaggtgga acccctggat aaaaatcgcc tcaaggtcgt
    12181 accgccctct tttcgtcccg atctcacccg ggaggaggac cttgccgagg aggtggcgag
    12241 gctcaacggg tatgaccagg tccccgtgac catgccgtgg gtcgcgaccc agagcgcccc
    12301 tctggaccct catatgaggc tgcgccacga aaccaaggcc cttttgcagg gctgcgggtt
    12361 tctcgaggtg ctgaactatt ccttcatctc ccgggactgg ctgagaaagc ttcggctccc
    12421 gcccgacgac ccccggctgg accccattgc cgtcctgaac cccttgagcg aagaccaggg
    12481 ggtcatgcgc accagcctca ttcccggcct gatcgagacg gcacgccaca atttcgatca
    12541 tcgaaacgac gacctgcgga tcttcgagct tagcaaggtc ttcttgaagc gggagggaga
    12601 agtcctggcg gacgagcctc accgcctggc cgggctcatg gcagggaaaa gggtccccgg
    12661 cgccttgtac gggtctgccg aatccgtgga cttcgccgat gtcaagggag tggtggaaga
    12721 ggtcctggaa ctgtttcgaa tcccgggagt gcggtacgcg gcccgggacc tgcctcccta
    12781 cctcgacccc taccggtcgg caaccctttt ttgcggctcg gacgggatcg ggtccctggg
    12841 acgactccgc gaggaggtcc gagaggcctt tgatcttcga aatccggcgt ttgtgttcga
    12901 cctggatttt gatcggttgt attctctcct gggacccccg agtcgtttcc gggccctgcc
    12961 caagtttcct tccgtcgccc gtgacatggc cctggtcgtg gatgaggagc tcccggccct
    13021 ggagatccaa gacttcctcc tgcagcagca ggaacccttg atggaacagg tggagatctt
    13081 cgatatctac cgacatcccc aaatgggtcc cggcaagaaa agcctgggct accgggtggt
    13141 gtaccgtgcc gcggaccgga gcctcaccga cgaagaggta aacgcgatcc acacccgcct
    13201 ggcggaaaag gtcctccgtc agtttcaggc aacattgcga tagtggggag ccatgagcga
    13261 aaaggagaat cccggcctca aaggttcgga caagttgccg caagccaagg ccccttgcga
    13321 ggtcatctat acctggggga acaagtccta cgtggggaca tccacccatt ttcacgagaa
    13381 agggatgctc atcttctgcc gacaaccggc acccctcaac gaacgggtga aagttaccct
    13441 gcggtttccc gacttcaaga acgtcctgga aatggaggcg gaagtagtgt ggaccaacat
    13501 tcacggtccg tccgacacct ttcgcccccg ggcaatgggg gtcaagttcc tgaacgtgga
    13561 acgggacgcg gaaaggctcc tggccgagct ggcctcccag tatgaggccc acgggagcat
    13621 ctacagctgc tactacacct gaggtgtcgc ctcggtgggg cattccccgg cccggggcgc
    13681 ttcagggaac atcctttccc cggggtcctt cgtcgttccc ccggctcctc cccgtcccgg
    13741 gtggggaaag gcctcctctg tcctgcaacg acgggatgaa gcttgaaatt cctcgaaatc
    13801 tttctggtgg caacggctct ggggtgtgac gcattcgcgg tggggatggg cgtcggcacg
    13861 aaattctgcg cccctcgaca ggtctttcgt ctttcctttc attttggcct gttccaattt
    13921 ctcatgcctt tgctgggctg gtttgcagga cagaaccttg tgggaatcgc ccgccagtgg
    13981 ggcccctgga tcgggttcct gctgctgttc ttcatcgggg ccaagatgtt ctatgagagc
    14041 ttcggcggtc ccgaagaaga gaggaaggag gagtgtcggg accccacccg gggattttcc
    14101 ttggtgatgc tgtccgtggc caccagtatg gatgcgctgg gggtgggctt cagcctgggg
    14161 atcctgggac aaagcctcct gacggcggcc gtttggatcg ggatcaccgc ctgtctcatg
    14221 acatggacag ccatgaagct ggggaatcgg ctgtcggaga aattcggaaa acgcatggaa
    14281 accgtggggg gtgtgatcct catggcgatt gccgtcaagc tggtcaccct gtgacaagga
    14341 agcgggaacc tatggcagtc aaacttgaaa tcgccctgaa agaagacctg ttcgacgcgg
    14401 agggagcggg actctgccga aagatcctgg attatttcgg ctggaaagta ggaactgccc
    14461 gagtcgttca catcctgacc ctggacaccg ccctttcccg ggaggaattg gaagcggttc
    14521 gtaaggagat cttcaccaac ccggtcaccc agtgctcggg gtttcattcc ctggcggggg
    14581 aattcgactg ggccatttgg gtggggtatc ggcccggggt gagggacacc gccggaagcg
    14641 tggccatgga agccatggcc gactacctgg gcaggtcttt tgaaccctgg gaggccgctt
    14701 acacctcgaa gctgtacctc ctgggcaacg cctccctgga tcaatcccgg gtggaagtca
    14761 ttgcccgaga gcttctcgcc aacgagatca tccagcagtg gcgggtgtat tcccgggaat
    14821 cccgggaccg ggaccagggt atcgggatca tcgtccccaa ggtggtgttg gaccacgagc
    14881 cttcggtccg gacgctcccc atcccctcgg acgaggaact gatgaggctc agccgcgaac
    14941 gcaacctcgc cctcaatccc caggacgtcc cggtcatccg gagctatttc acgcgtccgc
    15001 acgtgatgga agcccgaatc agggcaggat tggctttacc cacggatgtc gagctcgagg
    15061 ccatatccca ggccagaagc gatcactgca atcacaacac ctttcgaggc aagttccact
    15121 accgggacct cggaacgggg gaaagctgcg tcgtagacaa tctcttcaaa tcctgtatcg
    15181 aagcacctac cctcgaaatc cggcaaaaaa aggactgggt tatttccgtc ctgtgggaca
    15241 acgccggggt ggctcgattc gacgagaatc accattatgc cgtcaccggg gaaactcaca
    15301 acagcccgtc caacatggaa gcctacggtg gctcccttac gggcattgtg ggcgtctacc
    15361 gggacatcat gggcacgggc aagggcgccc ggctggtggc gggcttgtat gggtattgcg
    15421 tggctccccg ggatcatgcc gggcccctca agccccatct tcatccccgg cgcctcctgg
    15481 acgggattgt ggagggggtg cgcgacgggg gcaacaagca cggggtaccc accgcctcgg
    15541 ggatcctctt ttttcacccc tcctacctgg gaaagtgcct ggtgtttgtg gcctgcctgg
    15601 gaatcatgcc ccgggaggtt cacgggcagc ctacggccga aaagtgtccg cagccggggg
    15661 actggatcct catgagcggg gggcgcgtgg gaaaggacgg gattcacggg gtcacggcgt
    15721 ccagtgagag ctactcggaa cacactccgg ccggtcacgt acagatcgga gacccgtaca
    15781 cccaaaagaa aatgcacgat ttcttactcg aggcccggga ccggggatat atcaccttca
    15841 tcacggacaa cgggggcggc gggctttcgt cttccatcgg ggaatcggcg cggcttgcgg
    15901 ggggggcctc agtggaattg gaaaaggtcc ccttgaaata tgccggcctg gatgtgtggg
    15961 aaatctgggt ctccgaatcc caggagcgga tgaccgtcgc cgtgagcccc gaacacctgg
    16021 atcgttttct ggaactgtcg cgaaagcacg aggtggaaag cacggtcatc ggtcgatacg
    16081 aaaattccgg gaagcttcac ctgacgtatc acggccgcac atgcgcctac ctggacctgt
    16141 ctcttttttc cgaggatttt ccccagtggg agttcgaggc cgagtggctg ccgccggaat
    16201 cccggggttt gtgggagccc gtactggggg aaccccggca gtacgggacg ctcttgcgca
    16261 ccctcctggg tcgccccaac ctctgttccc gggagtggat ccagcgacaa tacgaccatg
    16321 aagtgcaggg agccgcggcg gtgaagtttc ttgtcgggcg gcggcgggac atccccaacg
    16381 acgccgtggt ttaccgtccc gtgctcgagg gtcgcaaagg gttggtcatg gcccaggccc
    16441 tgctccccac cctctccctc atcgatacct acgacatggt ggcagccacc atggacgagg
    16501 cggtgcgaag aatcctcagc gtaggggggg gcctggaaga aatcgggggc gtggacaact
    16561 tctgctggcc caacatccag tatgatcccg ttacgaaccc cgacgggcga tacaaggcgg
    16621 cccagctggt gcgggccaac tgggcactga gggacgtctg cttggcctac ggcatccctc
    16681 tgctttccgg caaggacagc atgtacgtgg acgggcacct ggaaggagca ttcggggaac
    16741 gacacaaggt ttccgggctc ccgaccctcc agttcacggc caccgccacc atgcctgatg
    16801 tccatcgggc cctttccatg gagccgaaaa tgcccgggga cctggtctac atcctgggaa
    16861 tcacccggga cgagctgggt gcatccgagt actacgatct gttcggcctg gtgggacgca
    16921 acgcgccccg gttggacccg aggaccgtcc tgcctttgta ccgggccgtg gaacactgca
    16981 tcgcccgggg gcttctcgca tcgtgtcatg ggatctaccg gggaggactg ggaattcacg
    17041 cgtccatgat ggccctggga gccgagctgg ggctcgattt ggacttgagc cgggtgcctg
    17101 cggatcccgc cgcacttcgg gacgaccggg tgcttttcag cgaatcctgc gggcgcttcc
    17161 tggtgacggt gtctccttcc cagcaagagg atttcgaaga acagtttcgg aaccttccct
    17221 gcgggttgct gggccgggtt atcgaagaac tggtgctgcg ggttcgcgga cgttccgggg
    17281 acgttcttct ggaggaacct ctggcggagc ttcgacgggc ctggatggcc tcgaacggat
    17341 aggagaggag tggaacggat gcaaccggtg cgtgctctgg tgctgacggg tttcggactc
    17401 aattgcgacg tggaaacggc ctatgccctg gaaatggcgg gggcccggac cgaacgggtg
    17461 cacttgaacg gcctcatcct gggggaaaga cgactcgagg agttccagat cctggtgatc
    17521 ggagggggtt tttcctgggg ggacgaccac ggggccgggg tgatcatggc catgcggatc
    17581 aagcaccgca tgaaggacga cctcctggat tttgtggaca aggggggcat cgtcgtcggc
    17641 atctgcaacg ggtttcaggt cctggtaaac ttgggacttc tgcccggctt cgaaccccgc
    17701 cgattgaaac gggaggtggc gctgatccac aatgactgcg gcaatttcct ggaccagtgg
    17761 gtccacctgg cggccaatcc ttcctcgccc tgcgttctga gccggggaat ccaaaggttg
    17821 gagctgcccg ttcgccacgg ggagggaaaa ttctttgccc cgcccgcagt gctggatcgg
    17881 ctggccgacc aaaaacaggt cgccttcacc tacatccgcc ccgacggccg accggccgac
    17941 ggggaagccc ctcacaatcc caacggttct ttgggggaca ttgcgggcat ttgcgacccc
    18001 acggggcgca tcacgggcct gatgccccat cccgaagcgt tcaaccactg gaccaatcat
    18061 ccggattgga ccctgcacaa ggaaatagcc cggagaatcg gctcgtccct tcccgaggaa
    18121 ggggaggggg tccggatttt ccgaaatgcc gtggaatact ttcgctaaaa cagccgggga
    18181 gggaattccc gtttttcctc ttcacggaca tccccgggct gtggggtcaa cccgttgggc
    18241 gaggtcgagg ggcgggaaga ctcgtaaaga gatacggcaa tccccgcccg tcatccatcg
    18301 agtatggaaa ccacattgcc ccggccggtc cggaacgggt cgggagactt cggctcgggc
    18361 attcactccg gcgtttcaaa ggaacgagga tggcctcaaa ccgcaaaaac cttcgcctgg
    18421 caatcctgat ttcgggaggc ggcaccaatc tgcaggccat tctggatcgt tgcgccgccg
    18481 ggcttctccc tgcaaaggtg gtcgtggtag ccgccgaccg gcccgatgcc tacggcctga
    18541 ctcgagcccg aagggcgggg gttccttccc acgtggtgga ctattcccgc tatctctccg
    18601 gggccatgga ttcgaggatc tcacccgagt tggaagttct ggcggagagc ctggattcac
    18661 gccaacggat tctccgggaa tcggaccggc ccaaacggct ccggcgactg gtgcgcctgc
    18721 ttctggccga aaacgaaatg atccgggtga tcgagagcgg gcagccggat tacctatgcc
    18781 tggcgggctt catgaggctg gtgacgcctc attttctggc tcacttccac ggggctcgtc
    18841 cctggcgggt cctcaacatt cacccggcgc tgctgccggc ttttcccggc cggcgcggct
    18901 acgaggatac ctttgactat gggtgcaagt ggggcggggt aacggtccac ttcgtggatg
    18961 aaggggagga cacgggtccc attcttgccc aggccgtcta tcccatttgg cccgacgagg
    19021 acctcgaagc ggttcgatcc cggggactcg aactcgagta cgaactctac agccaatgca
    19081 ttcgttggct ttgggacgaa cgggtggaag tgcggcgtcg agaggacggc cgctggttca
    19141 cggtgattca tgacccgtcc tacccccgca tcctgaagga atgggtggac cgggccatgt
    19201 cttcggcctc cgggtcctga gggtacggtc cccacgggac ttctccggga agggcaaccc
    19261 ccctcgtccc atccgggtcc ccgaccctgg cgggtcgtcg gagaggcgaa gggggtgcgc
    19321 ccgtggagcc ctcacattcc ctgcaattca cctatttcca acaaaacgtc tcttcggttt
    19381 cctcttccca cccatcccac aggtatgctt cctcccgacc ccgggcgatc acccggaagg
    19441 cgtagcgcac ctgttcctcg tagaacgcac agtagggact gggcgcgttc agagtgcccg
    19501 aaacggcatg cacctcggca gggcagggcg ccccgcagaa atgacgaatg gcgcagttgc
    19561 cgcacgggac gattctttcc gctgtgcggg aggtcacgtc ccggaagggg ctgccgccca
    19621 ggatggatga cagggaattc ttgaagagat ttccgccgtt aaaccgggga aaaccgatga
    19681 actcgctgca cggaaacacc tccccccggg cggaaacggc aaagaaacag cggcctcctc
    19741 cgcacggggt gatgtcgcac atgagccgtc ggctggtggg gcccagaatt gccgccagga
    19801 tgttggcaaa attggccgtc accagcttgc ggcccgtctc ccggtacagc tcttcggctc
    19861 ggtccagggc ccggaagaaa tggcgggcca gcacggaatc ctccggcttg agatccaggc
    19921 ccccctgtcg ggtgacccgc accgggttga acaagacggt cctcacgccg tgtccgtggt
    19981 agaagtccac catggcgggc aggctttcca cattgacacg ggtcacggtg gtgatcacgt
    20041 tgaacgccgg gtacggggag agcttctcga tgaccgacac cacgttcgcg aaggcccccg
    20101 cacccttcca gttcttgcgg gtgcggtcgg ccaggtccgc cgttggggca tccagggata
    20161 tgccgatgcc cactccgcga tcctcgagga atgtgatggc ttcgtcgtcc aggagggttg
    20221 cattgctctg gatgccgaag acaaagtcat cgcgatactc atcgattcca gcaaagacgg
    20281 cctcccgggc cagcaagggc tccgagccgt gaaaaatcag ctggggcttc actcccggag
    20341 gcaggaccga ggagaagtaa tcccgaagga ttcccagggc ccgggacaat tgcgccgccg
    20401 tcatggtcgt gccgtttcga cgcatttcct cgggcaggta acaatagctg cagttgaaat
    20461 tgcagcgctc ggtggggttg aaatacacgg cggagggctt gagaccgaag cgcagttggt
    20521 gcatctcccg ggagagggat cccgccgctt tccggaactg gtccaccagt ccgcctgcca
    20581 aggcctcctc gagggcctcc cgccggacca cggcccaaaa ggcccggtct gcctccacca
    20641 ccgccacctg gtcgggaagg ccaatctcca gtaccgaaaa gttcggtccg tatcccgtgt
    20701 tggcgtatag aggacggtgc tcgtattctc gtgcagggtt cataagcgtt cctagttctt
    20761 cttcttcgct tggggggtca acagaatgta gtgcgaaaga ccggttcccc ggccgttctc
    20821 gcgacaccga cgccgatacg aaacgactct ggctttcttt tccatggatg cttcctcctg
    20881 tttgcggttg ggttgggctc cacaaaaaaa cccgggaagg ccgatatact tccaggcctt
    20941 tccgggttcc ttttaccatg gggaccccgt tgggattgtt ccagcaggtc ttctggcttt
    21001 cggatcctct gaagatccgc caccttcccc ccgaggtggg cctcgagagt ggccgagcgg
    21061 gcactcgcac gaatcttcat ccccgattac agcggcggga ccgccacgga atttcaccgt
    21121 gttccgagat gctggagaca tcaacggttt cgaggagctt tcattagacc ggcccgaagg
    21181 agatgtcaag aggttttccc gtcccggcgt cggcgtcggc aaagacggca cccgcggaac
    21241 ggttcccgct ccgtctttct cccctgattc ctgcataaat ttgccatatt ccctttgccc
    21301 tggcggaaag ggtggatata gttatccaca aacctgaaac gacggggcgg gaatcggcga
    21361 tggatgaacg catctccgaa ggcgggggtc gcccggttcc ccgcgtatcc attcacgatc
    21421 ctgccgggga ggtcatgcac tatggcgcaa aagaaaaagg tcgtggagaa tgcagggaag
    21481 gagaaagccg accctacatc ctgcgaaacg aaatcctgta acttcatctg cagtcgggac
    21541 accctggacg gggcctaccc ggccctgatt cttgccgtca acgcccggcg cctgggaatg
    21601 gaggccggca tcttttacac cttcatgggc gttcgcctca tcttgaagga cggcatccgg
    21661 aaggcccagt acatcccgcc gggtttcatg ggcgccatcc cgggcatggc cgccgtggcc
    21721 accaagatga tgcgcaagca aattgacgag gcgggcattc cgtccctgga ggaactcctg
    21781 gagatggccc agctcgaggg ggtgaagctg gtggcctgca agatgaccat cgatatgatg
    21841 aaactcaagg aagaagattt tattgaaggc gtggaaatcc agacggccga agactttctc
    21901 aagcacgccg tgacgtgtca aatcaacctg ttcacgtagg tttgggtttc gtggacccgg
    21961 ccggttgaac tgtgggcggg tggacgggcc gagcggttcg gtcgaagtgc gcgggaacgg
    22021 acgacgaaga cgggctgcgg accggggaag ctgccgctcc gaccatggcg tttccccggc
    22081 agcccccgtg ggagggacgg gatcccctcg gtcgcggaca ccacttcact ggccgctcgg
    22141 agcggtggga tgcaagggcc ccgccgtacc gtaagctccc gccggcaggc atctccggca
    22201 cccgttacct ggctcggacc tggccggagg cggatgggga tagggtccgg ttgttttgtg
    22261 ccttttcaga tacagggcat tggcgaatgg ccgcctgttt ctcgtccatt tcctgtttgg
    22321 tccgaaaaac ccaggcactg gggtccgagt agcagtaaac agccagttcg cattccttac
    22381 attcttccat gatcacggtt ccttgggttt ccgatcgggg tgagacgggt cgtccgttcc
    22441 ccgggagagg ccctgcagat actcgtccca ttccaccggc agcaggtatt ttttccgact
    22501 gttgcattgc ttgcatgaag gcaccacgtt gccccgggtg gatcctcccc cccggacaag
    22561 gggcaccacg tggtccatgg tcaactcgag ggaaggcaca cgtttgccgc aatagtggca
    22621 ggtacccttg gccagttgcc tctgccacca ttgcgtcttg cgaagggctt tggccttttc
    22681 cttttcccgg cgcagcgcct cgggagatgc cgacacctcg aaatagggat tgcccgaacc
    22741 cgtcgtcatg gtgtttgcgt cattcctagg acttgggcgg cttcagagcc acgtagaagg
    22801 tattctcacc cctcttgacc agcagcagca ggctttcgcc cttcttggtc ttttcgagcg
    22861 cctgcttgta gtcccgcagg ttctgaatct tgtgtcggtt gacttccttg atcaggtctc
    22921 ccgtgcgcaa tttggcttcg gcaccggggc tgcccggctg aatcccggag atcaccacgc
    22981 ctttctcgcc ctcggaccag ccgaaccgct gggccagttc cggcgtcagg ttttgaaccg
    23041 tgaggcccca tgcggactcc tttttgacgg cagggggttc cgcgacctca tcgggcatgg
    23101 tgccgatggt cactttgatc tctctgggct tgccgtcgcg caaaacggat atggtgaccg
    23161 cggtgttggg ggccgtagcc gccaccatgc gggaaaggct cggagagttc tccacttcct
    23221 tgccgttgaa cttgaccacc acgtccccgc gcttgagttc ggccttgtcg gcaggaccgt
    23281 cggccaccac gtcggccacg atgactccct tggtttcctt gatgccgaag gactgagcga
    23341 gctcgggagt gatgtcctga atcataactc ccagccaacc gcgaatgacc ttcccggact
    23401 tgagctgggc aagaatgtcc ttggcgacat tgatgggaat ggcaaagccg attccctgtc
    23461 cctgggccac gatagcggtg ttgatgccca cgacctcgcc gttcatgttg aacaggggac
    23521 ctccgctgtt tcccgggttg atggccgcat cggtttggag gaagtcatcg tagggtcccg
    23581 caccgatcac acggcctttg gcgctaatga tccccgccgt cacggtatga cccaggccga
    23641 agggattgcc cacggccatg acccagtctc ccactcgaat gttgtcggag ttgcccaggc
    23701 gggcgggctt gggaaaggcg gaatccggag tcacccggat gagggccaaa tccgtcttgg
    23761 ggtcccgacc cacgaccttg gcgtcgtact ctttcccgct ctcgatcttg atcttgatct
    23821 cgtccgcttt ctcgaccacg tggttgttgg tgaggatgag gccttcctcg tccatgatca
    23881 gcccggaacc aagggcctgg gttttcatct ctccgtgggg cgtatccccg aagaaacgct
    23941 tgaagaattc atctccgaag aactcccgaa aaggggaatg ggggctgaaa aacggttgca
    24001 tggggttttc tttgagcacc tgggtcgtcg aaatgttcac cacggcgtgt ttcacgtgct
    24061 ccgacaagtc ggcaaacgag gggggaccct gggccccgaa gctgggaagg gccgcctgga
    24121 catccggact gctccagccc acgaatgcca acgaagccag caacagacaa cagacggacc
    24181 cggcaacagc caacacctta ttgactttca tcaaggctcc tttctaaaca acagggttcc
    24241 ctcgggggac atccccgtat cgtagcagcg ggtcgtgcct tccccgggaa tccaatctcc
    24301 gtgaaccgac agggaaaaac agagaagagg cacgcatcgc ctcttcgggg tgtatttcgt
    24361 atcctgttgc ggtttcgaag ctgcactctc caaacaatct ccaatccacc acgcgctttc
    24421 ctacccggga cgaaaatgat ataggtgtct aacaataaat caattggacc aaaaagcaag
    24481 atggagttga ccagggagaa tccgcagtgg aacgggatcg cctcatgctg accgaggcct
    24541 gcgatcaggc catccaactc gtttctcaaa cccgcgagat ggagaggctt taccaaaaag
    24601 ccgtccaatc ggtgggggaa ggggccctgc gaacgggaat cctgggtttg gccgtggatg
    24661 ccatggagga tgaggaactc aagaacgagg tctttgtcaa tgacgagagc ctgctgagct
    24721 tcttttgcgg gatctggatc caatacttgc tggtggaagt cgccggcatc acccgggaga
    24781 agctcaaagc cctcgcagcg gatgcgttga gacagctcca gaaagacaag cgagtccact
    24841 aaagtcgact gcggtttacg tccgccgtcc gggttctttc atcggcctgg cccggacact
    24901 gtagaacggc tcccagagac gttccctgta gtcatccaga tttttcccga catctttccc
    24961 cctttcccgg agcagtatcc tgcgcgaatc aaatccctgg agaagcactg ccgggttcgg
    25021 ccgacccctg ttccaacaag catcctcctt accttcgacg gtcacccacg gctgccgccc
    25081 caatccgtac gcgcggtaat ccccgggtga cgtcttcttt cctgtcgttg cattgccgac
    25141 actgtcggag gaagaatccg gtttaggagt cggcttttct agcgccaacc tgccggggat
    25201 tcggtgctcg tgcggatgct ccgcggcggt tccccccgtt cgtaatcctc gatggcaact
    25261 cgtttttcgg gaaggagcgc gaaaaggctc cgagcctcgg caaagtcgcc cagctgaaca
    25321 ctcatgcgca cacaaggcaa atcgtgcttg tccacgaggc tttccacgag ccgaatgtca
    25381 aaggaaccgg gaagacttgt gagggattga aggagggaca tgggggcttc ctggaacaca
    25441 accaggtgga agatcaaggg gtgaggccag ggattccttg cgtcccattc gacctccacc
    25501 agttgctggg gctgaagtcc gtggcgttga accaagtccg aacagctctt gtgatgaaag
    25561 gtgatgcctc tctcgctcag tgtggccatc acatccttct gtccgggaaa tgggttgcag
    25621 catcgggcaa acttgtggac cgccttgtcc agttcctcga ccctcaagac attggtttcg
    25681 ccgccggagt cggattcccg cgaaccctcc tcgtaataaa gcaccaggtg aggagagagg
    25741 aggtcctggc caatcttcgt gtagatctcc gtcgaacctt tgaggttcag gacctccgaa
    25801 accagcctga gcttctcctc atccagggca tccgggggaa aaccgtggcg ctccaattcc
    25861 tgatgcagga tctggcggcc agcattttcg gcatagcgac gccttttctg ctgcaagcgc
    25921 cggttgacgg ccgctcgcgc cttgggagtc ttgcagacgg attccagctg caggtccgga
    25981 tccaggggat ccgaagagac gacgatctcg accacgtctc cgtcttttag gggatgggtt
    26041 gaagggaccc acactccgtt gatcaaagct ccctggcagt agtcgcccag ttccgaatga
    26101 atccgatagg cgaaatccaa aacgacgctc cgcttgggca agtagtagat gtcgccgtcg
    26161 ggcgagtaga cgaagatctc ctcggcctcc gagagccgaa tgagggcttt gcgctgggga
    26221 cccgctcctc cgtactctcc tatggagcgc agcagttcgc tgatttcctg ccaatgctcg
    26281 tcgctcagcg ccttttcggt atcccattcg tgcaggatcc ccagagagga ccagtgatcc
    26341 atttccgagg ttcgaatctt gatgaggtag ttctgacctc ccacatgaat gcgcacatga
    26401 aggctccggt acccgttgtt cttgggattg gcaatgaagt ccctcacggt cctggggatc
    26461 ggggtgaagg tcgtgttcac ccgtcccagg gcgcaataac aatcgaggac gttgtcggtc
    26521 tggagaacaa ggacaaagtc gacgtggttc tcggtgttgc ccaggtccaa agtccttttc
    26581 aacggattgt agtagctgcc cagacctttg gtgcgcatcc ggacggcggc gggaggggag
    26641 gggggggccg aaaaagtccg ctgcaaggtg ttctcgatgt tcagcaggtc cgtagagccc
    26701 cgaagttccc tcaggtactg caggattttc cgactctttc ggggatagag aaaggagagg
    26761 gcgaggtggt agagttccct cttgagggag aagaggttga accgagctgc gatgggcgca
    26821 taaacctcga gggtttcatg agcgatgcgc tgccgcttgg tttggggcag atagtggagc
    26881 gtccgaagat tgtgcaggcg atcggccagc ttgatgatca ggacccccag acgacgactg
    26941 gcgctgagga agatcttgct gtgagtgaga tctctgaggg tgggacggtc caggtggctg
    27001 cgggtcagct tggtgcatcc gtccacgagc tcggccacgg tgggaccgaa ttcccgttca
    27061 atatcctcga tggtaatgga aggaacgtcc tccaccacat cgtggaggag ggccgaggcc
    27121 agaagatggg cgtccctgaa gtggaactcc ctcgccagga tctcggccac cgcgcacgga
    27181 tggctgatat aaggagcccc ggaccttcgt cgctggcccg tgtgaagctt ccgggcaaac
    27241 tccaaggccg caaaaaagac cgcgttctct tcccggcacg ggtccaggta cttggccatc
    27301 cgagcgtgat acagggacat gtcgaagaaa tggggctcac cggacacagg agcgggcagg
    27361 gcagggcggc cttttctttc ggttgcttga gagtccacag gctcctcatt tccggctcac
    27421 attcacccat tgggaaagcc cggcgacgac gggctcggcc acggagatgg tctccgttct
    27481 ccattccact gctgaattat aaggcataca aggattcccc aacaagcatg tcgagttctt
    27541 ccccggcgaa aaaattccca agagaggaaa gctgtcgagg cttttgtccg tcaatactcg
    27601 catgccgggg gtccgtaggc ccggagaccc gccttcacac agtccattga ttttctcaat
    27661 aaaatcatgt aacattgggc cattccgtgc taggcaactg atgcacaact cggaaggaac
    27721 gccgggagcc cgcttcgtcc gtgcgttcgg tttgataggg gaaccccgcc actcaagagg
    27781 aaaatgcgcc tatgaactat gagtcggttc tcaccacctg tacctattgc gggtgtggat
    27841 gcggcatgta cctccaggtc ctcgacggcc gcatcctcaa caccctccct tccaaaacca
    27901 gccccgtgaa cgaaggaaag ctctgtgtca aggggtggta catccacgaa tttgtacagc
    27961 accccacacg actcacaaaa ccccttctcc gggaagacgg gtctctccgg gaaacgtctt
    28021 gggacaccgc tctggattat gcagcatcgg agctcaagag gatccgtgac gcccacgggc
    28081 ccgacagcat tgcgttcctc acctcggcca agtgcaccaa cgaggacaat tacctcatgc
    28141 aaaagttcgc ccgggccgtg atcggaacga acaacgtgga ccactgcgcc cgcctctgac
    28201 actcctccac cgtggcaggt cttgccgcct ccttcggtag tggagccatg accaactcct
    28261 ttgcggagct cgaagacgcc gattgcattt tcatcatcgg ttccaatacc accgtcggtc
    28321 atccccttgt ggccacccgg attttccggg ccaagagcaa gggagccaag ctgattgtgg
    28381 ccgatcccag acagacccag atcgggcgtc tcgccgacct ctacgtacgg cagcggctgg
    28441 gaagcgatgt ggcgttgatt aacggcatga tgcacgtgat cctcaagaac ggatggcaga
    28501 accaggcatt tgtcgagtcg aggacggaga atttcgaggc gttcgcccag gtgatcgaga
    28561 aattcccacc cgagagggct gcggaaatca ccggggtgtc cgcggacgac atcgtccgca
    28621 tggcggaata ttacgccaag gccgaagccg ggtccatcgt ctattgcatg gggattaccc
    28681 agcacaccac cggcgtggac aatgtccgga gcctggcaaa cctgtccatg ctctgcggtc
    28741 acatcggaag gccgtccaca ggcgtcaatc ccctgcgcgg tcagaacaac gtccagggag
    28801 cctgcgacat gggcggactg cccaacgtct atccggccta ccaggccgta acggttcccg
    28861 aggtccggga gaagttcgaa aaggcctgga acgtgaagct cccttccaac gtaggcctca
    28921 ccatcatgga aatgatggac ggcatccaac acggaaagat caaggggctg gtcatcatgg
    28981 gggaaaatcc catggtgagc gacccggaca tccaccacgt gaagcatgct ctcgaggccg
    29041 ccgaatgcct ggtggtcctg gatattttcc ccacccccac cacggaactg gcccacgtgg
    29101 tgctccccgc cgcctccttc gcggaaaagg acggcacctt cagcaactcc gaaaggaggg
    29161 tgcagctggt gaggaaggcc gtggagcccc ccggggaagc caaggccgac tgggagatca
    29221 tccgggagct ttccaatcgc ctgggatatc ccatggacta caagtcgtcc gaggagatct
    29281 tcgaggaaat ccggaaggtt gcaccgtcgt atgcggggat gacctaccct cgactggaag
    29341 gcgaagggct gtgctggccc tgtccgacca cggagcaccc cggcacggcc tacctgcatg
    29401 cgggcaagtt cagtcgaggc ctggggttgt ttgccgccat cgactatcgc ccccctgccg
    29461 aggaacccga tgaggaatac cccttctggt tcaccacggg ccgggcttat gtccactatc
    29521 acacggggac catgactcgc cggtcgtcac acctcgatca ggagatgcct gagtccgtca
    29581 ccgaggtgca tccggaagat gccaagcgga tgagcctacg ggagggagac gtcgtcgagg
    29641 ttgccagccg ccgaggcagc gtcttttccc gggtgcagat taccgaccgg atcggaaaag
    29701 gggtggtgtt catgccgttc cattttgcgg agagcgccgc caataccctg accaacgcgg
    29761 ccctggatcc cgtcgccaag atccccgagt acaaggtgtg tgccgtcaag cttgcgaagg
    29821 tggcttgacc ggacccttct ccattgcctc tcgagccagc cggtcgcatc gttcgttttc
    29881 gaggtgaccg gcatggcctt tgatccagtt ccaatgaatc tcgtgcctct gggtaagggc
    29941 ttccagcgct tcccagaggt ctttgttttt cacgggggtt ttttcgctcg ttttccatcc
    30001 gttttgcttc caccggccga tccaccgggt gattccgtcc cgcaggtacc gagagtcggt
    30061 gtaaaggacc acccggcacg gttccttgag ggctcgaagg gcctcgatga ccgcggtcat
    30121 ctccatgcga ttgttggtag tgtgcggaga gtacccggag atttccttct cgacgccccg
    30181 gtatcgcaga atggctcccc agccgcccgg cccgggattg cccgagcacg cgccgtccgt
    30241 gaagatttcg accttcttgc ggggctggag agtcatgatc ccgtgggctg gggcttcagg
    30301 caaagggaga cagtcgccgg cggatgaagc gctcaatggc ggtggaagtg cgcttctcca
    30361 acacctgggt caactccttc aagggaatct ccgcccgggc catggcctgg tggcctccgg
    30421 cactgcccag ggattcgaag gcccttcgca cgattttgcc tgcatccttt cgatagccgt
    30481 cgttgcgaac gaccactacc aagttgtctt ggacaatgcc ggaagtgatg ctccaggaaa
    30541 tatcgtggac tttgaggaag aact
//
LOCUS       DAOOVC010000036        30525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1017_length_30525_cov_5.900591, whole genome shotgun sequence.
ACCESSION   DAOOVC010000036 DAOOVC010000000
VERSION     DAOOVC010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 30525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30525
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1017_length_30525_cov_5.900591"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLM79_06890"
     CDS             complement(<1..1183)
                     /locus_tag="PLM79_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOV86071.1"
                     /translation="MSREVSYQERLGLAKELADRIEERKDDLLESAARDAGFSVKVTA
                     IELDLAVRHLRTLEEEVPQVEGGIPYGVVGAIFPYDAPLVMLARLGGSALLSGNRLRF
                     SFSSRTPQTARVVAEIARPFHFLDPIVGTDNRAFGQRCVEDEAVRVLFLSGSSSVGEA
                     YRQNREAFDKLFFAGPGGMPAAMVLPGADAAAAAGFIARRAFVNGGQYCTTLKKALIH
                     TNLYEEVRSRILQEVRALRVGDPRDPRTDIGPIRVERTRSAVRAALEACPPSAVLEGA
                     LDGEWIRPLVVELDEIPDWELFGPFLALKPFTDVDEAVREVTRTDYGFLLAYFGTPPA
                     WARDAFVENFGMVHDNPDFTFTPLRLPFGGKKRSGWILERSAHGWVERDGAFLYSEEL
                     VK"
     gene            1402..1998
                     /locus_tag="PLM79_06895"
     CDS             1402..1998
                     /locus_tag="PLM79_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOV86072.1"
                     /translation="MSHPYRDHYFHKAKKENYLARAVYKLEEIQKKYRILKTGDRVLD
                     LGAAPGSWMQFSSSVVGPSGLVVGVDLKPIVHPFPEQVIAVQGDVFDGDFQEKLVNDY
                     GCFDVILSDMAPATSGIRSADSARSELLFEEALRVTRVALKTHGHFLAKIFQGPDFHT
                     LLTRVKGQFSRVKVVKPDASRKFSKEIYILAMDFKQRL"
     gene            2011..2760
                     /locus_tag="PLM79_06900"
     CDS             2011..2760
                     /locus_tag="PLM79_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOV86073.1"
                     /translation="MSGHSKWSTIKHKKGAADAKRGKIFTKLIKELMVAARMGGGDPN
                     GNPRLRAAINAARAENMPKENIDRAIKKGTGEIEGASYEEVNYEGYGPGGVAVLVDVM
                     TDNRNRAASDIRHIFSRNGGNLGEAGCVAWMFSKKGSIVFNKERISEEELMEMALDAG
                     AEDVVDAGDQFEVVTAIEDFSAVRSAFEERGLVYELAEITMVPQSTVRIEDEKTAQQV
                     LRLMDALEDSDDVQHAYANFDIPDNILNQLA"
     gene            2787..3290
                     /gene="ruvC"
                     /locus_tag="PLM79_06905"
     CDS             2787..3290
                     /gene="ruvC"
                     /locus_tag="PLM79_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOV86074.1"
                     /translation="MIRTIGIDPGSRYTGYGLMEGDGSQLRHLDNGTIRVPTRFAFPD
                     RLKYIYDELSSVIGRFRPTDMAVEEVFFAKNVQSALRLGQARGAAMLAGVNAGLAMYE
                     YSALQIKQAVVGYGRAGKDQIVQMIRHLCCLREAMDPNAADALAAAICHLNTRSSRAR
                     WNDSRPV"
     gene            3287..3883
                     /gene="ruvA"
                     /locus_tag="PLM79_06910"
     CDS             3287..3883
                     /gene="ruvA"
                     /locus_tag="PLM79_06910"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOV86075.1"
                     /translation="MIAYLEGKLRHKSPDHLVVDVHGVGYCVHVPLSTFYDLPDLGQT
                     ACLYIHTHVREDALQLYGFRSQAEKEMFLHLVSVNGVGPKLAVSILSGITPDELRRVV
                     LQQDSKRLRSIPGVGRKIAERLVLELRDYLRIKKEDIEGPVCLHGEDGPYADAYSALL
                     NLGYRSQEASRALQTARKTLDTDPTLERLLKEALRLLA"
     gene            3921..4940
                     /gene="ruvB"
                     /locus_tag="PLM79_06915"
     CDS             3921..4940
                     /gene="ruvB"
                     /locus_tag="PLM79_06915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOV86076.1"
                     /translation="MVDRVVEPRPREEDLPHENSLRPRTLAEYIGQTAVKENLAVFIE
                     AARLRSEHLDHVLFHGYPGLGKTSLALVISNELGVNLRSTSGPVIERPGDLAAILTNL
                     EPRDVLFIDEIHRLNHVVEEILYPAMEDFKLDIIIGQGPSARTIKLDLPPFTLVGATT
                     RAGLLSPPLRDRFGVSLRLEFYKVEELRTILLRSAKILGVRIDRDGAAEIAKRSRGTP
                     RIANRLLRRVRDFAEVRADGVITRSVADQALKMFDVDEKGFDRMDRNILRTIIEKYDG
                     GPVGIETLSAALSEEKDTLEDVYEPYLIQEGYLNKTPRGRSATRLAYEHLGIKTRPGQ
                     QMRLY"
     gene            4990..5541
                     /locus_tag="PLM79_06920"
     CDS             4990..5541
                     /locus_tag="PLM79_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86077.1"
                     /translation="MSTDVVVHSTQELQDKKTKRSFPLSGGKLVLAFLVLLSIGGIAV
                     TNIARDYGLYYWLSMVPLFALGSLFEGWSNARKEGIPAIRILGIQLSHWLASAVAVSL
                     VYYLEASGRISRETAGLMALSTLALATFLSGVYFSWRFCVLGVILGFTVVAAAFVEEY
                     FWIVIPPILLIGAVVLLWKRRRP"
     gene            complement(5728..7338)
                     /locus_tag="PLM79_06925"
     CDS             complement(5728..7338)
                     /locus_tag="PLM79_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86078.1"
                     /translation="MKTNGSDEVSVSLPHRLRRWLVVDFFGIVREMRRSYLPPLMVYL
                     AAGVSGFTGIIESFYVKEQLGLSAEFLAGLGFWAGLPWALKMPLGHLVDLYWRWKSFF
                     VYLGAALMALSLLIMVGLTGFPAWMSLYLDADTWYVLSTLISPVGFVLQDVVADAMTV
                     EAVPAFEEDGSPVPEKELQRRHVTMQTLGRIAIVGGGAMVAGLGGWLATVFSYRTMYE
                     ISLVIPLLSVCGVLLGGLSLRRRARGLRREGLPEDRIRVMLGGHNPNLKPDAYILGAG
                     GFFVVLSVVLGLSALPMKKEIVFLTSLGVIAYLIWRLLRGMPPDKRRDIVSIAVIIFV
                     FRAMPGFGAGAGWWEIDVLGFDEAFFGTLRQISSILTILGMFALRGWMARRPIPYLVV
                     FLSAYGTVMLSPFVGMYYGLHEWTQQVFGFGARTIAIIDTMADSPLGQVAMIPMLAWI
                     AREAPVDRKATYFAVMAAFTNLALSASSLGTEYVNSIFVVPRGDYTELGRLMITVSII
                     NLVVPILTVLAFRFPGKKDRRPPDLAAS"
     gene            7609..8715
                     /gene="msrA"
                     /locus_tag="PLM79_06930"
     CDS             7609..8715
                     /gene="msrA"
                     /locus_tag="PLM79_06930"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOV86079.1"
                     /translation="MKTAHLIIVVVMTLTVFPALLGLGKGQSSPQTFRDNPAKATFAG
                     GCFWCIEADFEKLPGVLDVVSGYTGGTRGNPTYKEVSAGDTGHVEAVQVTYDPAKLSY
                     GQLLDFFWKHVDPTDPDGQFVDRGTQYRTVIFYHDEEQRRLAEESKRELNRSGRFDKP
                     VVTEILPLKQFYRAEDYHQDYYRKNPLRYSFYRFNSGRDQFLGKIRAESEAKSGGPGR
                     QSRYSKPDDETLRKKLTPLQYRVTQQEGTEPAFQNEYWNNKRPGIYVDIVSGEPLFSS
                     LDKYDSGTGWPSFTRPLEPSNLVEKEDRGFFTVRTEVRSRHADSHLGHVFPDGPPPTG
                     LRYCMNSAAMRFIPRENLEAEGYGEYVGLFSEGR"
     gene            complement(9387..9716)
                     /locus_tag="PLM79_06935"
     CDS             complement(9387..9716)
                     /locus_tag="PLM79_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86080.1"
                     /translation="MRFDDPGGGSATDLDYPVTPAVSLIEEWRIAFLPHLYPFVEHGG
                     TKHASHFLKVSDRKLPICVEAGICSLERISTNGGKRGFPVEIDPEDLRRLPVVADASR
                     PRQRGGG"
     gene            10130..10885
                     /locus_tag="PLM79_06940"
     CDS             10130..10885
                     /locus_tag="PLM79_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HOV86081.1"
                     /translation="MSQKTAPPKSLEDLPDSEIVSWVLEGFRRTLVHYGYWFGEVETR
                     LGTEKAVKVETEAGDLWWGIMLKRLSFVLGFEIEDNVPKALKSMDRKELLGLLDAVCI
                     NWLADDGVWFQAVEKRYGMDAAKACNDACWSRFSPYEALRTKEILGLPDFPGLEGLKT
                     ALGFRMYARINKQVIEEVDEHTVVFRMVDCRVQAARKRKGLPDYPCKSAGMVEYPTFA
                     SAIDPRITTECIGCPPDEHPEDWYCAWKFTLIP"
     gene            complement(10923..11150)
                     /locus_tag="PLM79_06945"
     CDS             complement(10923..11150)
                     /locus_tag="PLM79_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HOV86082.1"
                     /translation="MKTENEFVYSFPKNPLEEVRAGLTVFRGKQYVDLRIYYKGDDGE
                     FHPSKKGLTLSIELFPELEEAVKRLREVVAE"
     gene            11506..12075
                     /locus_tag="PLM79_06950"
     CDS             11506..12075
                     /locus_tag="PLM79_06950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV86083.1"
                     /translation="MEERIRTLGTLCAKHFTAQLDTVRLRTGRITERIKVDHPEAAAV
                     VPLLDENHILMVRQWRYAIDRETLEIPAGKADPGEDLETCAHRELLEETGYDAARLLH
                     LFSYYPAIGYSNEVIRIYAASGLRKVSDNGDEDEIARTEIVSLDQIRRFILDGEIQDG
                     KTVIGISLLEARQARGDLPRDFFAPGPQP"
     gene            12072..12542
                     /locus_tag="PLM79_06955"
     CDS             12072..12542
                     /locus_tag="PLM79_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HOV86084.1"
                     /translation="MNKVPITRLGYLRLLGELLYLRRAVRPQVLEELQEARSYGVKAE
                     NQQYLLARERHVILQKKIRELEEKLARCEVVVGRKFFLKQAAFGAVLVVQNIDTGETR
                     RYQLVGPYESDVRDGKLSVESPVGRCLMGHREGEEVTVYTPSGIRIYRILSIQV"
     gene            complement(12525..14186)
                     /locus_tag="PLM79_06960"
     CDS             complement(12525..14186)
                     /locus_tag="PLM79_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV86085.1"
                     /translation="MSLRSRAFTDEPMDSFHRFEEICRAMSDPGFYPHSVSSIQRTDT
                     HISAVFLTGRWVYKLKKPVDFGFLDFRTLEARRHYCLQEVLLNQRLSRGVYEEVVEIR
                     RSNDGSFSLEAEGEVVEYAVKMRRLSEKMSLVSLLERNRVSSRQMIRLGAHLAAFFDG
                     SARSADIDRFGSPSVIRYNMEENFRQTESFVESFLEKGPWEFLRQVSRTFFEDHMEMF
                     ALRVGEGRIRDGHGDLRADHVYFFRGIQIIDCIEFNDRFRYGDAVADLAFLHMDLEHR
                     GYASQSRDFLEAYAREARDPQVYALLDFYAAYRAMVRVKVACLRSTEVAKEERGPLRK
                     DANTYLWQAYRYAFQFARPTLWVLCGLPASGKSSLALKLSETLHLPVFQSDLVRKEGG
                     GEARIEAGVVPYGGGIYRAELRQRVYGRLLASAQEQLKGGRSVVLDASFSRRKWREEA
                     ARLARDLNTSLVFVECVCGEETLRRRLEQREEAPGLSDARLVHLPAMVRDFEPLTEVP
                     PLQHGRVCTEQDPDRVLVETISNGYFLKCAQVRELLQGAGSDLNG"
     gene            complement(14183..14956)
                     /gene="folE2"
                     /locus_tag="PLM79_06965"
     CDS             complement(14183..14956)
                     /gene="folE2"
                     /locus_tag="PLM79_06965"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOV86086.1"
                     /translation="MIDVQNQLDPRRIRVDKVGVKDIRYPMTVMDKNDRTQNTVASVN
                     MYVNLPREFKGTHMSRFIEILNDFHGHLDIREFSKILEAMQEKLQAESAHLEVCFPYF
                     MEKLSPVTATPGLMEYGCRVSGSLDRNRGYDLVLEVNVPISTVCPCSKEISDFGAHNQ
                     RGMVRLAVRFKRFIWIEDLVKLVECSASCDVYSVLKRPDEKFVTEKAFENPKFVEDVV
                     RDIAKELKEDPNVLWFRVDVENFESIHNHSAYARIERQK"
     gene            complement(14972..15763)
                     /locus_tag="PLM79_06970"
     CDS             complement(14972..15763)
                     /locus_tag="PLM79_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV86087.1"
                     /translation="MKIAVSGKGGVGKTTLAAFLAKWFAREGKSVLAIDADPDANLGH
                     GLGLHGAAKIVPIAEMKDLIAERTESTPGSFGGFFKLNPKVDDLPGMLAVPIEERIRL
                     MVMGGVKKGGMGCVCPESTLLKNLVQHLILRRNDVVIMDMEAGIEHLGRATSQAVDCL
                     VVVVEPGRRSVDTASRIKELAAEIGLTRLGVVGNKIRGDRDRDFLLAKLSGHRFLGFI
                     PYDESIIEADLRGDFARDVQPATREALEEIARNLADMQATTTGNP"
     gene            complement(15862..>16212)
                     /locus_tag="PLM79_06975"
     CDS             complement(15862..>16212)
                     /locus_tag="PLM79_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOV86088.1"
                     /translation="REIPDGHFYFDAFQRRARDPFLSTFGREAALFRACAEALGGTPV
                     DNGDVGMAFRVLPNITIQIVLWEGDEELSPEATILFDASIEQGLPAEDIAVLSGMLVY
                     RLMGIARTRQTRSV"
     assembly_gap    16213..16312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16313..16664)
                     /locus_tag="PLM79_06980"
     CDS             complement(<16313..16664)
                     /locus_tag="PLM79_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HOV86089.1"
                     /translation="MARIDDYRESFRLAAERLVKADLPRVAKFSGADLTVLENGTCRL
                     RLLFFGTPYLVCVNDGVEVIREGKNREVPLPEKILICHYLERARGRPSSDQLITFREI
                     PDGHFYFDAFQRRAR"
     gene            17054..17512
                     /locus_tag="PLM79_06985"
     CDS             17054..17512
                     /locus_tag="PLM79_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV86090.1"
                     /translation="MAKAVETFIGRCENCGAKDRIPGPRLAERALCGKCGKPPPSSGS
                     YPELARLISDASFRKEVLDFSGPVVVNFHSPTCPYCRRFDATFQQLVSKYAGRIKFTK
                     IVREQNPITASRLGVQGVPTLLLFREGVLKDRTVGLLPEADVEAHLMRIL"
     gene            complement(17609..19225)
                     /locus_tag="PLM79_06990"
     CDS             complement(17609..19225)
                     /locus_tag="PLM79_06990"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOV86091.1"
                     /translation="MPEFKYQEPFPQTKDETKYRLLTKDYVSVTSLDGQQILKVDPAG
                     LSFLANQAFREVSFKLRPAHLEKVAAILNDPEASPNDRGVAVALLRNAEIAAKCELPL
                     CQDTGTATIVGKKGQQVWTGGNDEEALSLGVYKTYTEENLRYSQTIPLTMYDEINSGT
                     NLPAQIDLYATDGAEYKFLFVAKGGGSANKAMLYQETKALLTPANLEKYLVEKMKTLG
                     TAACPPYHVAFVIGGTSAETCLKTVKLATTGYLDNLPTKGNEHGQAFRDLELEAKIEK
                     AAAKAGYGAQFGGKYFALDVRVIRLPRHGASCPVAMGVSCSADRNIKAKINKDGIWLE
                     QLEDNPGRFIPEKYRGKHEHGVVKINLNRPMKEILAELTKYPVTTQLSLTGTIIVGRD
                     IAHAKLKERIDRGEGLPQYLKDHPIYYAGPAKTPKGKPSGSFGPTTAGRMDSYVDLFQ
                     ANGGSLIMIAKGNRSQQVTDACKKHGGFYLGSIGGAAALLAEENIKKVEVLEYPELGM
                     EAIWKIEVEDFIAFILVDDKGNDFFKQLSC"
     gene            19748..20515
                     /locus_tag="PLM79_06995"
     CDS             19748..20515
                     /locus_tag="PLM79_06995"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HOV86092.1"
                     /translation="MEIRVLGSSGSEGPGRNPTAFLIDDFLLLDAGTVSLSLDLESQT
                     KITHIFLTHAHLDHIKAIPFLVDNIVATHGLGKITILSGRDVIADLKRNVFNNKIWPD
                     FTLLPSSHNPIMRYQVVSTRKPVEVRDYRIFSTRVSHSVPAYGYVVADSSGEALVYTG
                     DTGPTDRLWKRIKGFKVKALIIETSFPDEMSRLALDSGHLTPSLLHKELRKLPVPPDH
                     IYITHLKPYYREEIERQLEKLDGIPLTVLSDNLRFSL"
     gene            20743..23880
                     /locus_tag="PLM79_07000"
     CDS             20743..23880
                     /locus_tag="PLM79_07000"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HOV86093.1"
                     /translation="MAILKAFFERKLHLLILLGLTAFLLSLFPPPLLLSPTSIAGGDT
                     ASHLPSAAVMRHRLLSGGPLFAWVPGNYAGFPLFLNYFPLPFILIACLSFLVSLPIAF
                     KVGTLLAVIPLPLAMFGCLRNLGCPHPAPALGAAFSVLFLAMKDNSMWGGNLTSTLAG
                     EFSYGISLILAVYLVGKLFRDVPRRRSLIANAVLEALVAFSSGFPVLQAGFGSFYFLR
                     RLRHWPYLIVLHALAAGMIGFWLLPLVWRLPWTTPAHHTWHFQGWWEAVPVILWPAVF
                     GILLGWGVQAYARLKTPRTPGDPQDGRPQEYLWFQVGVSLLFFACAPWIGLADVRFLP
                     FTQILLLLLGAIGWGEFLNRFPYSRITSCLLVVGVVLGSQPALRETRSWIEWNYSGFE
                     AKELWPVFQQINSALKGTENDPRVFYEHSALNDRLGTARAFEMLPYFSGRSTLEGLYM
                     QSSLSSPFVFYLQSELTQSPSCPFQGYSYSRVDAVRAAQHLRLFNANQVITVTRCVSD
                     LLDQCPDYALQRSIPPYRIYEVSNFEPAYVVAVRYLPHRIPAAGWKATQYEWFRRSSL
                     EVPLVVTPESPDGDYWLQLPLWEGSMEQIPIIPLARAGEVQVTSTLEGDRIRIWTSHL
                     EHPLWIKVSYHPDWHVAEGAGELYAASPAFLLLFPRSHRVTLQFQPRGGVYSIGTTTS
                     LIAWLGSLALVGWSRWKGRVPGTGETGSFFKGGTSRDTRVFGPIPKAGLQDGNGKGSD
                     EVGPFLPAWAWVLLGMSIPAVLFWRGHEDPILLFHRGVKAYDQADAMPAVSGGPPSSD
                     ITTGKARFSAARRHFARCLERFPNSPVVDHAFHYFVLTFMKEDRLQTVIDLVQSFLIR
                     YPDSRFRPQALYYSGLSSSGLQWMESARGFYWQVLRQFPEDPVAPSAAARLIELDSPQ
                     SMLSAARELYAQDRHLQALPILESLRGSPIPEIRSQAMLLSGYCRFYLSHWEEASRLL
                     PAWLQEFPGHSDTATVLSMLAESRMALGQYPEALESLRQAMALDPSMARSFSGMALLK
                     HLEDLVSISGKAMEP"
     gene            24136..24885
                     /locus_tag="PLM79_07005"
     CDS             24136..24885
                     /locus_tag="PLM79_07005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV86094.1"
                     /translation="MSSPLSDPFDFTVLIPLYNEESSLIPSVHRLLEFFVRHGLRGRI
                     ILGSNGSTDHTVLVGELLEDSYPEIVRFFHTETRGAVGDVFRKAVDLASSPILISMDI
                     DLSVDVDFILESLALLESHEIVLGSKQSGYQLRSVWRRLGSGVFLLCARLLLQLPYND
                     YSLGAKAYRLDSIRPWLDRLSADTNYVLDLVYQCHHARMPIVLLPVACVDWRKSRFSL
                     IREAIVRFSHLFDLWFRHHLHRNRNSPGGCS"
     gene            24923..25834
                     /locus_tag="PLM79_07010"
     CDS             24923..25834
                     /locus_tag="PLM79_07010"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOV86095.1"
                     /translation="MRVWIHSDDAGISLSSTQRILTAWKEGLIDGFSILANGPAAPVV
                     YDRLAADPRTPARLSVHLNLSEGPCSAPPETVPSLLDREGNLNCGFLTLWTRWVSGSA
                     ESRTRWLEQIEREWRAQIFRVAQGVAPRPIMALDGHNHLHMLPFLFGTTLGLAREHSI
                     PQVRFSMEPRHVPRPHFRLLSFPFWINGLKFLVLRVCCRAARERFSIPRELRTIPIVG
                     ILSSGRMTARSAVAALEGARRDRIPEIECVFHVGRMEAGEVESWKCRLRARRFFSSPW
                     RQREYEELSALDWSSHGFHRGFHSSRP"
     gene            25797..26759
                     /locus_tag="PLM79_07015"
     CDS             25797..26759
                     /locus_tag="PLM79_07015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV86096.1"
                     /translation="MVFTEVFTARDLDYEAHSDLMRRAFHALFSRAAISNDFMSPAYY
                     RWKYRPPAGEGRTAVVFHGNRMVATSSMFPLHLKHPGGMSRVWQSCDSATLPEARGAG
                     YFTRCLEALIEILPPGEIFFGLPNPSSRRGCEKVGWRPQQVIRTWVGFSRPGFGLPAR
                     FSGRFHAELGEHWDRFRPRDRPVLSRDPAYLQWRYLENPCFRYRFFTDISPEGFGFAV
                     ARLGSFRRIPFGLVLEWWAREPAVQKALFGQVSRWTLEHGAPLIMTLNSRLRKTDALR
                     NGLLPMPECVLFKNQVLMGTARDPEGQKLFREKWDFQLGDWDGF"
     gene            complement(26775..29027)
                     /locus_tag="PLM79_07020"
     CDS             complement(26775..29027)
                     /locus_tag="PLM79_07020"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOV86097.1"
                     /translation="MKPKCKILQTFLSWEVVFVFCSTLLLTALYFLVVPFLEFVELKA
                     WDLHFRQRGTVEPSGDFAFVAVDEESVNRDGRWPWPRRQMARLIEAVQDHGAKVIGLD
                     MGFFEPDLKLRRNAVLDVRSRIEKGMPPGAGKELVRWLETIAQEEDDDLILSDTLQRL
                     KVPLVLGHFFYFDKDQFVPEPPPTELLEKAQVPIVRTMGSPRVGRLNEAVGLEANIPV
                     IEQSSRYMGSFNVFSDPDGTVRWMPLIFRFQDRIFPSLAMQMLAVAFPNSPLMVTLDT
                     RGVRNIRLGSVSIPTSNKGELLLNYYGPAYTFPHFSATTLMHHESPPDCLKDRIVVIG
                     NTTLGLYDMRPTPFSMTFPGVELHCTVMENIARQHFLQRIERNMPLVDLAAIFGLAGV
                     FLLMQFFIRGLPLAGVVLFLFGGYILLTHWLFLQGGLWLNHVYPTMNLAVAYLGTSVH
                     RYLREEKEKRMIRNTFSLYVHGSVVEEMLANPERLRLGGEKKELTVMFSDIRGFTTLS
                     EQRPAEELVPQLNEYLTRMTQVVFDHHGTLDKYIGDAVMAIFGAPLPQKDHPHRACAT
                     ALDMIRVLKGLQQGWKERGLPVLEIGVGINTGLMMVGNMGSERRFDYTVLGDNVNLAS
                     RLEGLTKMYGVTIVVNESTWEAAQDRFVGRVLDVVRVKGKQKPVTIHQLMDNGENAAA
                     HREPLRLYGDGIRFYQEMRWEEALRRFLEVEKFWPGDPPSRLYQGRCRHLLERKPSEE
                     WSFVTTLDHK"
     gene            complement(29275..>30525)
                     /locus_tag="PLM79_07025"
     CDS             complement(29275..>30525)
                     /locus_tag="PLM79_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86098.1"
                     /translation="GTAVPYGGPTLAAGVVYYVRVKTFDGNSWGAWTECPHLYTTYYC
                     KGDVNGDGIWDELDYHLIGIPGVYLPVGQDDPLYVFTGQYSYTPSIQRILWYDNLYDK
                     DPLTGEYLEYPMTPWVMPGYGFWFITVPDRTITAGGTAVPEDCEYYLTVYPGWNMLAH
                     PFLSSTPWSQVLVFDGEIYYPLDDPGNVWVQQVAYGYDMGYFPVETGAYAPLITGSQS
                     NLEPWNGYWVYNYSNMEVALAIFPTGALPPEEPAYAPPAKRLVKARPKDEPRPKGISF
                     SLSEARRKYADRTLLLGLDPRASAGPDFLDALAPPPVSANVPRIYVDHRKWETRKGKY
                     ARDFRSTGKFPARFPLVMEVPARQEATTYVLRWEGIEGLSAKRKVMLKDPAVGRRVDM
                     RTKNAYRVTVPAGETRHKLVVTIR"
BASE COUNT         5996 a         9342 c         8888 g         6199 t
ORIGIN      
        1 ttttcaccaa ttcctcgctg taaagaaagg ccccgtccct ttccacccag ccgtgggcgc
       61 ttcgttccag gatccagccg cttcgctttt tccccccgaa gggaagccgc aagggagtga
      121 aggtgaagtc cgggttgtcg tgcaccatgc cgaagttttc cacgaacgca tcccgggccc
      181 atgccggagg agttccaaag taggcaagca ggaagccgta atcggttcgt gtgacctccc
      241 gcaccgcttc gtcaacatct gtaaaaggtt tgagagccag gaaggggccg aagagctccc
      301 agtcgggaat ctcgtccagt tccaccacca agggacggat ccattcgccg tccagggcac
      361 cctcgagaac agccgaagga ggacaggctt cgagagcggc ccggacggca ctgcgggtgc
      421 gttccactcg aatgggaccg atatccgttc gagggtcccg ggggtctccc actcgaaggg
      481 cacggacctc ctgcagaatg cggcttcgca cttcctcata caggttcgtg tggatgaggg
      541 ccttttttag ggtagtgcag tactgcccgc cgttcacgaa tgcccttcgg gcaatgaatc
      601 cggcggccgc cgcagcatcg gccccgggaa gaaccatcgc cgcgggcatc ccgccggggc
      661 ccgcaaagaa gagcttgtcg aaggcttccc ggttttgccg gtaggcctca cccacactgg
      721 aagaaccgga caggaagagc acccgaaccg cctcgtcttc cacgcacctt tgaccgaagg
      781 cccggttgtc cgtccccacg atcggatcga ggaaatggaa gggccgagcg atttccgcca
      841 cgacccgggc cgtttgggga gtccgggacg agaagctgaa gcgcaggcgg tttcccgaca
      901 ggagggcgct gcctcccagt cgggccagca tgaccagggg agcgtcgtag ggaaagattg
      961 cccccaccac cccgtaggga atgcctcctt ccacctgggg gacctcttct tccagggtcc
     1021 tcaggtgccg taccgccagg tccagttcga tggcggtcac cttcacggaa aaccctgcat
     1081 cccgggcggc ggattccagc aggtcgtcct ttctctcctc gatccggtcg gccaattctt
     1141 ttgccaaacc caatcgctcc tggtaagaca cttcccgtga catgatctct cctgttttgg
     1201 gagttttgac tgcggatcgg caataatata cggcttcggg gacacgattg gcaaagggga
     1261 acccgggagg gggacgggtc gcccggtcga ctcgggaatt cccgggaccc cggcggtgcc
     1321 cggaagggca tgtttacaag gcccgctgct ttgtgttata ggagcattcg gaaaaaataa
     1381 aattttttag gcggttagtc catgtcccac ccttatcggg atcactactt ccacaaggcc
     1441 aagaaggaga actatcttgc ccgggccgtg tacaagctgg aggaaatcca gaagaagtat
     1501 cgaatcctga aaacggggga tcgtgtgctg gatttgggtg ccgcccccgg ctcatggatg
     1561 cagttttcga gctcggtggt gggaccgtcc ggcctagtgg tcggggtcga cctcaaaccc
     1621 atcgttcatc ccttccccga acaggtcatc gccgtgcagg gagatgtctt cgacggggat
     1681 ttccaggaga agctcgtgaa cgattacggc tgcttcgacg tgatcctcag cgacatggcc
     1741 cccgccacca gcggcatccg atccgccgac agcgctcgat ccgagctgct gttcgaggaa
     1801 gcgttgagag tgacccgggt tgccctcaag acccacggtc atttcctggc aaaaatcttt
     1861 caaggtcccg attttcatac cctcttgacc cgggtgaagg gccagtttag ccgggtgaag
     1921 gtggtgaagc ccgatgcctc tcgaaaattc agcaaggaga tttacatcct tgccatggac
     1981 tttaagcaac gactgtgagg agtggatttc atgtccggac attccaagtg gagcaccatc
     2041 aagcacaaga aaggggctgc ggatgccaag cgcggcaaga tctttaccaa gctcatcaag
     2101 gagttgatgg tggccgcccg catgggcggc ggggacccca acggaaatcc ccgactgcgg
     2161 gccgccatca atgccgcccg ggcggagaac atgcccaagg agaacatcga ccgggccatc
     2221 aagaagggca cgggggagat cgagggggcg tcctacgagg aagtgaacta cgagggctac
     2281 gggcccggag gggtggccgt cctggtggac gtcatgacgg acaaccgaaa ccgagcggcc
     2341 agcgacattc gacacatctt cagccgaaac ggcggaaacc tgggtgaagc gggctgtgtg
     2401 gcatggatgt ttagcaagaa gggttccatc gtcttcaaca aggaaagaat ctcggaagag
     2461 gagctcatgg agatggccct cgacgcgggg gccgaagatg ttgtggatgc gggagaccaa
     2521 ttcgaggtcg tcacggccat cgaggatttt tccgcagtgc ggtcggcctt cgaagagcgc
     2581 gggctggtgt atgaactggc cgagatcacc atggttcccc agagcaccgt tcgcatcgaa
     2641 gacgaaaaga ccgcccagca ggtcctccgg ctcatggacg ccctggaaga ttccgacgat
     2701 gtgcagcacg cctacgccaa cttcgacatt ccagacaaca tcttgaacca gcttgcatga
     2761 ggggcgacac cggagtggcg ggcggcatga tccgaaccat tggaatcgac ccggggtcgc
     2821 gatacacggg ctacggcctc atggaaggag acggcagcca actccggcac ctggacaacg
     2881 gcaccattcg agtccccacg cggttcgctt ttccggatcg cctaaaatac atctacgacg
     2941 aactctcctc ggtgattggc cggtttcggc ctacggacat ggcggtggaa gaggtgttct
     3001 ttgccaagaa cgttcagagc gccctgcggc tgggtcaggc ccggggtgcc gccatgctgg
     3061 cgggggtcaa tgcagggctg gccatgtacg aatacagcgc cctgcagatc aagcaggcgg
     3121 tggtgggata cggaagggcc ggcaaggacc agatcgtcca aatgattcga cacctgtgct
     3181 gcctgcggga agccatggat cccaacgccg ccgacgccct ggcggcggcc atctgccatt
     3241 tgaacacccg ctcgtctcga gcgcgctgga acgattcgag acccgtatga tcgcatacct
     3301 ggaaggaaag ctccggcaca aatccccgga tcatctcgtt gtcgacgttc acggggtggg
     3361 ctattgcgtc catgtaccat tgagcacctt ctacgacctg ccggacctag gacaaacggc
     3421 atgtctttac atccacaccc acgtgcgcga agacgccctt caactctacg gcttccgctc
     3481 ccaagccgag aaggagatgt ttctgcacct cgtttcggtc aacggagtcg gtcccaagct
     3541 ggcggtgagc atcctatcgg gaatcacgcc cgatgaactg cgcagggtgg ttctccaaca
     3601 ggattccaag cggctgcgga gtattccggg agtgggacga aagatcgcgg agcgtctcgt
     3661 gctcgagctt cgggattatc tgagaatcaa gaaggaagac atcgaagggc ccgtctgtct
     3721 ccacggggaa gatggtcctt acgcggacgc ctattccgcc ctgctaaacc tgggctaccg
     3781 aagccaggaa gcctcccggg cgttgcagac ggcccgaaag accctcgaca cggacccaac
     3841 cctggagcga ttgttgaaag aggccctgcg ccttctggca tgagcgtctc cggggcctcg
     3901 ccccgggagg ataggagcca atggtcgatc gcgttgtcga accccggccc cgggaagagg
     3961 acctgcccca cgagaacagc ctccgtcccc ggactttggc ggaatatatc ggacaaaccg
     4021 ccgtcaagga aaacctggcg gtgttcattg aggccgccag acttcgatcc gagcacctgg
     4081 accacgtcct ctttcacggg taccccggcc tggggaaaac ctccctggcc ttggtcatca
     4141 gcaacgagct gggggtgaac ctgcgcagca cctcgggtcc cgtcatcgag cgtcccggcg
     4201 acctggccgc catcctgacc aacctcgagc cccgagacgt cctgttcatc gacgaaattc
     4261 accgcctcaa ccatgtggtg gaggagatcc tctatccggc catggaagac ttcaagcttg
     4321 acatcatcat cggccaggga ccgtcggcaa gaaccatcaa actcgatctg ccccccttca
     4381 ccctggtggg cgccactacc cgggcgggac tcctgtcccc tcccctgcgg gaccgcttcg
     4441 gcgtatccct gcgcctggaa ttctacaagg tcgaagaact ccggaccatt ctcctacggt
     4501 ccgcaaaaat cctgggggtc cgcatcgaca gagacggcgc cgcggaaatc gccaagcgtt
     4561 cccggggaac cccccgcatc gccaatcgtc tgctgcgccg ggtgagagat tttgccgaag
     4621 tgcgcgccga cggcgtcatc acccggtcgg tggccgatca agccctgaaa atgttcgatg
     4681 tggacgaaaa aggcttcgat cgcatggatc gcaatatcct gcgcaccatt atcgaaaaat
     4741 acgacggcgg acccgtgggt atcgagaccc tgtcggccgc tctttccgag gagaaggata
     4801 ccctcgagga cgtctatgag ccttacctca tccaggaggg gtatttgaac aagacgccgc
     4861 ggggccgctc cgccacccgc ctggcctacg aacacctggg gatcaagact cgtcccgggc
     4921 aacagatgcg actctattga tccgaccctt ttgctcactt tcaacgttca cacctatgga
     4981 ggaaccggta tgtccacaga tgtcgttgtc cattcaaccc aggagcttca agacaaaaaa
     5041 accaagaggt cctttccgct ttcggggggc aagctcgtcc tggccttcct ggtcctcctt
     5101 tccatcggcg ggattgcggt caccaacatt gcccgggact acggcctgta ctactggctg
     5161 tccatggtgc cgttgttcgc cttgggcagt cttttcgagg gatggtccaa cgcccgcaag
     5221 gaaggaattc cggcaatccg gatcttggga atccagctct ctcactggtt ggcgtccgcg
     5281 gtagcggtgt ccctggtcta ttacctggag gcctccgggc gaatcagccg ggaaaccgcc
     5341 ggactcatgg ccctctcgac cctggctctg gccaccttcc tttcgggagt ctacttttcg
     5401 tggcgctttt gcgtactggg cgtcattctg ggatttaccg tcgtggcggc ggcctttgtg
     5461 gaggagtatt tctggatcgt cattcctccc atattgctga tcggggccgt cgttttactc
     5521 tggaagcgac gaaggccttg attggcgagg cgtgccctcc atttcaccca gggttcccgg
     5581 ggccggctcc gctgtgcggc ccggcccggg gggcgggacc tcgaacccgg cagatcaccg
     5641 ggggggcatt cccggccggc ccggccgttc atccggcttt ggtattcagg acggggtgag
     5701 accccgggga gctccgggga cccgttctca ggaagctgcc aaatcggggg gcctcctgtc
     5761 tttctttccc gggaatcgaa acgccagaac cgtcagaatg gggaccacca ggttgatgat
     5821 cgaaacggtg atcatcaggc gtcccagttc tgtgtaatct cctcggggaa ccacgaagat
     5881 cgaattcaca tactccgtgc ccaaactgct ggcggaaagg gccaggttgg tgaatgccgc
     5941 catgacggca aaataggtgg ctttccggtc cacgggcgct tcccgggcaa tccaggccag
     6001 catgggaatc atggccacct gccctagggg agaatccgcc atggtgtcaa tgatggcaat
     6061 ggtacgggct ccaaaaccga aaacctgctg ggtccactcg tgaaggccgt aatacatccc
     6121 cacgaagggc gagagcatga ccgtgccgta cgcggacagg aacaccacca ggtaaggaat
     6181 gggccttcgg gccatccacc cccgcagggc gaacatgccc aggatggtca agatggaaga
     6241 aatctgccgg agggtcccga agaacgcctc atcaaagccc aggacgtcga tctcccacca
     6301 gccggccccg gccccgaagc cgggcatcgc tcgaaaaacg aagataatca cggcgatgga
     6361 gacgatatcc cggcgtttgt cggggggcat ccccctgaga agccgccaaa tgaggtaagc
     6421 gatgacccca agggaggtga gaaacacgat ctcttttttc atgggcagcg cagaaagccc
     6481 caacaccacc gagagcacta cgaagaaccc cccggccccg agaatgtaag cgtccggctt
     6541 caggtttgga ttgtgccccc ccagcatgac ccggattcgg tcttccggca gcccttcccg
     6601 acgcagtcct cgggctctgc gccgaaggct gaggcccccc agaaggactc cacacacgga
     6661 tagaagagga atcaccaggg aaatctcgta catggtccgg taggaaaaca ccgtggcaag
     6721 ccagcctccc aatcccgcaa ccatcgcccc ccctccgacg atggcgatgc gccccagggt
     6781 ctgcatggtc acatggcgcc gctgaagctc cttttcaggg accggagagc cgtcctcctc
     6841 gaaggccgga acggcctcga cggtcatggc gtcggcaacg acgtcctgga gcacaaaccc
     6901 gacgggagag atgagggtcg aaagaacata ccaggtgtcg gcgtccaggt agaggctcat
     6961 ccaggccggg aatccggtga gccccaccat gatcaggagg ctcagggcca tcaacgccgc
     7021 tcccaggtac acgaagaagg atttccaccg ccaatataga tccaccaggt gcccaagggg
     7081 catcttgagg gcccagggca aaccggccca gaatcccagt cccgccagaa actcggcgct
     7141 gagtcccaat tgctccttta cgtaaaagga ctcgatgatc cccgtaaaac cggaaacccc
     7201 cgccgccagg tagaccatca agggcggcag atagctccgg cgcatttccc ggacaatccc
     7261 gaaaaaatcg accaccagcc aacgccggag tcgatgcggc agactcaccg agacttcgtc
     7321 gctaccgttc gttttcatga gttcatggtt ccccaaacca aatcggtccc gtcttggaat
     7381 atccctccgg catcggtcga gttccgcatg ccatttcctt cccccggcct cggaaaacag
     7441 atccgaacca tactgcaagg taaggtcgat caccagcttt ttccggcctc ccggcgaggg
     7501 gggggtcccg tatgcgggct ctgcgccgcg acaaaatttt ctccggaatt caggcttctg
     7561 cagcaaatcg gcggattgga tcttattatt tctacggagg taccaaccat gaaaaccgct
     7621 catctcatca tcgttgtcgt aatgacactc accgtcttcc cggcactcct ggggctcggg
     7681 aaggggcagt cttcccccca gaccttccgg gacaaccctg caaaggcaac cttcgccggg
     7741 ggatgtttct ggtgcatcga ggcggatttc gagaagctcc ccggtgtgct ggacgtggtt
     7801 tccgggtata ccgggggcac ccggggaaat cccacttaca aggaagtctc ggcaggggac
     7861 acgggtcacg tggaagccgt ccaggtgact tatgatcccg caaagctcag ttacggacag
     7921 ctgctggatt ttttctggaa acacgtagac ccgacggacc ccgacggtca gttcgtggac
     7981 cggggaacgc agtatcgaac ggtcatcttt taccacgacg aagaacaacg gcgactggcg
     8041 gaggaatcca agcgggagtt gaatcggtcg ggacgctttg acaagcccgt cgtgacggaa
     8101 attcttcccc tcaagcagtt ctatcgagcg gaagactatc accaggacta ttacaggaag
     8161 aaccctttgc ggtattcttt ctaccgcttc aattccggtc gggaccagtt cctgggaaag
     8221 atccgggccg aatccgaggc caagtccggg ggacccggca ggcaatcccg gtattccaag
     8281 cccgatgacg agacccttcg gaagaaactg acccctctgc aataccgggt cacgcagcag
     8341 gaaggcacgg aaccggcctt ccaaaacgag tattggaaca acaagaggcc ggggatctac
     8401 gtggacattg tatccgggga gccgcttttc tcttccctgg acaagtacga ttccgggacc
     8461 ggttggccca gcttcacgcg gccgttggaa ccgagcaacc ttgtggagaa agaggaccgg
     8521 ggctttttca cggttcgaac ggaggtacgc agccggcacg cggactcgca cctgggacac
     8581 gtgtttcccg acggccctcc tcccaccggg ctgcgctatt gcatgaactc cgccgccatg
     8641 agattcatcc cccgagaaaa cctggaggcg gagggctacg gggaatacgt ggggctgttt
     8701 tccgagggac gctgagatgc gtgcagaaga acttgcggga gggtcggagt cgggtatctc
     8761 tcatcgccgg gcacctcaag ggcctgcggg tgtgcggaat gccggagcgc cgggaaaccg
     8821 acggggcccg ttgccttccc ggatggttcc gtgcaggcgg tctgaggcgt tgctcgtgat
     8881 cgtcgccgtt tccttttggc aagtactttc aagcatctct cggtttgccg gaaaccgggc
     8941 ctgacggcaa tccgagcact tttcgcaagt tgccccgggc atcggtgccc aaaggccgag
     9001 cccggggagg ggctttggtt attccaggga tgccccccac cattggtcgc accggaccgg
     9061 ttcccggaaa tgctctcgac cgtccccgtc cccatcgcac ccgagcaacc gaccccccca
     9121 gagattgttg tcgtctctgt tgacgaggtt attgaaccga cctgaggaca caagcgccag
     9181 ggcaaccccg ttgcctttgt tgtcatggat ggacctgtcg ttcgttgcca gggaagaatt
     9241 tctccggaca tgaatccccg ggccgttatt ggaatggatt ggatcctcga cctcgcccgc
     9301 ttccacccgg gatgtttgcc gtcgatgagt cgcgaatccc cactcttcct cgcaggccgg
     9361 ataggtttta cctcccggag tcggcttcat ccgccgcccc gttgtcgcgg gcgcgaagcg
     9421 tccgcgacaa cgggtaatct acggaggtcc tcggggtcta tttcaaccgg gaagccccgc
     9481 tttcccccat tggtggaaat cctttccagg gagcagatgc cggcctcgac gcaaatgggc
     9541 agtttccgat cggaaacctt gagaaaatgg gacgcgtgtt tcgttccgcc gtgttcgacg
     9601 aagggataaa ggtgcggcag gaaggcgatg cgccactcct cgatcaggct cactgcgggt
     9661 gtgaccgggt agtcgaggtc cgtcgcactt ccgccgcccg ggtcgtcgaa ccgcacggag
     9721 cgcatccttg tccgcggggt ctcgaagacg gtgccggctt acaggatgcc cggatgcaaa
     9781 ccgtgagcct ccgacaccat ggagtcgagg gctcgattga ggcggcccct cccccccttg
     9841 accgggcgga tgggagatga aaaggaaaag ggagtcggaa gggaggagcg atccgcttcc
     9901 cctcgggtga agaagggttg gaaaacacgg cccgagaggt ttaggatgcc gttctgtggc
     9961 gattcgggcc ggggagaggc cctcgtgaga ccctctccta ccggggttcc cgacctcccg
    10021 cccgagcacc cttgcgggaa cctgcgaaaa agccggatcg cccgctccat gactccggag
    10081 cacgccaggc atcccacgaa acccttgttt cgagaaagga gaaaagacca tgtcccaaaa
    10141 gacggcgccc cccaagagcc tcgaggatct gcccgattcg gaaatcgtct cctgggtcct
    10201 ggaaggcttt cgtcgcaccc tggtccatta cgggtactgg ttcggagaag tggaaacccg
    10261 attgggcacg gaaaaggccg tgaaggtgga aaccgaggcc ggggacctct ggtggggcat
    10321 catgctcaaa cgattgtcct tcgttttggg attcgaaatc gaggacaacg ttcccaaggc
    10381 gctcaagtcc atggacagaa aggaacttct gggattgctg gatgccgtct gcatcaactg
    10441 gctggccgac gacggggtgt ggttccaggc ggtggaaaag cgctacggca tggatgccgc
    10501 caaggcatgc aacgatgcct gctggtcccg cttctccccc tacgaagccc tgaggaccaa
    10561 agagatcctg ggcctcccgg atttccccgg actcgaggga ctcaagaccg ccctgggatt
    10621 tcgtatgtat gcccggatca acaagcaggt catcgaggag gtagacgagc acaccgtcgt
    10681 cttccgcatg gtcgactgcc gggtgcaggc ggccagaaag cgaaagggac tccccgatta
    10741 cccgtgcaag tccgccggga tggtggaata ccccaccttc gcttccgcca tcgatccccg
    10801 gattaccacg gagtgtatcg ggtgcccccc cgacgagcac cccgaagact ggtattgcgc
    10861 ctggaaattc accctgatcc catgaggccg tgcgggcagg ggaccctcga cggttcttcc
    10921 cttcattcgg ccaccacttc ccggagcctc ttcaccgctt cctcgagctc ggggaaaagc
    10981 tcgatgctca gggtcagccc cttcttggag ggatggaact ccccgtcgtc tcccttgtaa
    11041 tagattcgca gatccacata ttgtttgccc cgaaagaccg tcaagcccgc ccgcacttct
    11101 tccaggggat tttttggaaa ggaatagaca aactcgtttt ccgtcttcat ggtgactgtc
    11161 cctgtgaaca catgtgaccg cccttgccaa caccccggtc ctccccttcc tcccggaacc
    11221 accgggtccc tccccgtcga aaaatcggca ctaacaataa tcatgggccg aagtgggatg
    11281 caagccttat ttggaacctg cccgtaattg cccccagggg ggagaccggg caggatgttc
    11341 cttgatcccg gaaaagaaac atgtttttat ttccgggggc cgggaatccc ctttttgtgg
    11401 cctgtgccgg gttcccggcg ggtttttccc acgaacccgt tcgccccacc ggctgtcgat
    11461 ttccccggtg aacgattgac ccttcggaac agatcgaggc attttatgga agaacgaatt
    11521 cggactttgg gaaccctttg tgccaagcac ttcaccgctc aactggacac ggtgcgcctc
    11581 cgaaccggca gaattaccga gcgaatcaag gtggaccatc ccgaagcggc ggcggtcgtc
    11641 cccctgctcg atgagaacca tatcctcatg gtgcggcaat ggcgctacgc catcgatcgc
    11701 gaaaccctgg agatccccgc cggcaaagcg gacccgggag aagacctcga gacctgtgcc
    11761 caccgggaac tcctcgagga gacggggtac gacgccgcaa gactcctcca cctcttcagc
    11821 tattaccccg ccatcgggta ttccaacgag gtgatccgga tctatgcagc ctcaggtttg
    11881 cggaaggtct cggacaacgg ggacgaagac gagatcgcca ggacggagat tgtttccctc
    11941 gaccagatac gacgattcat cctggacggg gaaatccagg atgggaaaac ggtcatcggc
    12001 atttccctcc tcgaggcccg acaggcccgc ggagacctgc ctcgcgactt ctttgctccg
    12061 ggaccccagc catgaacaag gtccccatca cacgcctggg atatttgcgt ctcctcgggg
    12121 agttgttgta tcttcgtcgt gcggtgcgtc cccaagtact cgaagagctc caagaagccc
    12181 gcagctacgg tgtgaaggcg gagaaccagc agtacctgct ggcaagggaa cggcacgtca
    12241 tcctccagaa gaagattcgg gaactggaag aaaagctggc ccgctgcgag gtggtggtgg
    12301 gccggaagtt cttcctgaaa caggcggcat tcggcgcggt cctggtggtt caaaacatcg
    12361 atacggggga aacccggcgg tatcagttgg tgggacctta cgaatccgat gtgagggacg
    12421 ggaagctttc ggtggagtcc cccgtgggaa gatgcctcat ggggcatcgg gagggtgaag
    12481 aagttacggt gtacactcct tcgggaattc gcatttaccg cattttatcc attcaggtct
    12541 gaccctgcgc cctgcagcaa ttcccgcacc tgagcgcact tcaggaaata tccgttggaa
    12601 atcgtttcca caaggacccg gtccgggtct tgctcggtac aaaccctgcc gtgttgaagc
    12661 ggggggactt ccgttaaggg ttcgaagtcc cggaccatgg ccggaagatg caccaaccgg
    12721 gcatccgaca ggcctggggc ctcctccctt tgttccaacc gccgacgaag ggtctcttcc
    12781 ccgcagacac attccacgaa tacgaggctc gtattcaggt cccgggccaa tcgggcagcc
    12841 tcctccctcc atttcctccg ggagaaggaa gcgtccagca cgacggaacg gcctcctttg
    12901 agctgttctt gggcggaagc cagcaaccgc ccataaaccc gttggcgcag ctcggcccgg
    12961 tagattccgc caccgtaggg aaccaccccg gcctcgattc gggcctcccc gccgccttcc
    13021 ttccgcacaa ggtcggactg gaaaaccgga aggtgaaggg tctcggaaag tttcagggcc
    13081 agggacgact tgcccgaagc ggggagcccg cacaggaccc agagggtggg acgggcgaat
    13141 tggaacgcat accgataggc ctgccaaagg taggtgttgg catctttccg aagcggccca
    13201 cgttcctctt tcgccacctc ggtggaacga agacaggcca ccttgactcg aaccatggcc
    13261 cggtaggccg catagaaatc cagcagggcg tagacttgag ggtcccgggc ctcccgagcg
    13321 taggcctcga gaaagtcccg gctttgggaa gcgtagcccc ggtgttcgag gtccatgtgg
    13381 aggaacgcca ggtcggccac tgcatccccg tatcgaaacc ggtcgttgaa ttcaatgcag
    13441 tcgatgatct ggatgccccg aaagaaatac acgtgatccg cccgcaggtc tccgtgaccg
    13501 tcccggattc gcccctcccc cacgcgaagg gcaaacatct ccatgtggtc ctcgaagaag
    13561 gtacggctca cctgccgcaa aaactcccag ggccccttct cgagaaaact ctccacaaag
    13621 gattcggtct gtcgaaagtt ctcctccatg ttgtaccgga ttaccgaggg actgccgaaa
    13681 cgatcgatgt ccgcgctgcg ggcgctgcca tcgaagaagg ctgccaagtg ggcccccagg
    13741 cggatcatct gccgagacga gacccggttt cgttccagca ggctcaccag gctcattttc
    13801 tcggacagcc ggcgcatctt taccgcgtac tccaccacct cgccttcagc ctccagggaa
    13861 aaggacccgt cgttgctccg gcggatttcg acgacctctt catagacgcc tcggctgagc
    13921 ctctggttca gcaggacttc ctgcaggcag taatgccgcc gagcctccag ggtccgaaag
    13981 tccaggaaac caaaatccac gggttttttc agcttgtaga cccagcgacc tgtgaggaag
    14041 acggcggaaa tatgggtgtc cgtccgctga atggaggata ccgaatgggg atagaagccg
    14101 ggatccgaca tggcccggca gatctcttcg aaacggtgga aggaatccat aggctcgtcg
    14161 gtgaaagccc gggatcgaag gctcatttct gtcgttcgat gcgcgcgtac gcactgtggt
    14221 tgtggatgga ttcgaagttc tccacatcca ctctgaacca caacacgttg ggatcttcct
    14281 tgagttcctt ggcaatgtcc cgcacgacgt cttccacgaa cttgggattc tcgaaggcct
    14341 tttccgtgac gaacttctcg tccggccgtt tgagcacgga gtacacgtcg caggaggcgc
    14401 tgcattccac cagctttacc aggtcctcga tccagatgaa gcgcttgaac cggaccgcca
    14461 gtcggaccat tcccctctgg ttgtgggcgc cgaagtcact gatctccttg gaacaaggac
    14521 aaacggtgga gatgggaaca ttgacctcga gaaccaggtc gtaaccccga ttccggtcca
    14581 gggatcccga tacccggcag ccgtattcca tgagccctgg agtggccgtt accggggaga
    14641 gcttttccat aaagtaggga aagcaaactt cgaggtgagc ggattcggcc tggagcttct
    14701 cctgcatcgc ctcgaggatc ttggaaaact cgcgaatgtc caggtggccg tgaaagtcgt
    14761 tgaggatctc gatgaatcga ctcatgtgag tccctttgaa ctcacgcggc agattcacgt
    14821 acatgtttac ggatgccacg gtattctggg tcctatcgtt cttgtccatc accgtcatgg
    14881 gataccgaat gtccttgact ccgaccttgt ccacccgaat tcttcggggg tccaactggt
    14941 tttgcacatc aatcatgggt gctgcttccc actatggatt cccggtggtt gttgcctgca
    15001 tgtctgccag attgcgggcg atttcctcga gggcttcccg ggtggccggc tgcacatccc
    15061 gggcgaaatc tccgcgcagg tcggcttcga tgatggactc gtcgtacggg atgaacccga
    15121 ggaaccggtg accggaaagc ttcgccaaca agaaatcccg gtccctgtct ccccggatct
    15181 tgtttccgac cacgcccaaa cgagtcagtc cgatttcagc cgccaattct ttgattctcg
    15241 aggccgtatc cacgctgcgc cgacccggct ccaccacaac caccagacag tccaccgctt
    15301 gagaagttgc acgacccaga tgctcgatcc ccgcctccat gtccatgatg acgacgtcgt
    15361 tgcgcctcag tatgaggtgt tgtaccaggt tcttcagaag ggtgctctcg ggacagacgc
    15421 agcccatgcc gccctttttg actcccccca tcaccataag acgaatgcgc tcttcaatgg
    15481 gcacggccaa catccccggc agatcgtcca ccttgggatt cagcttgaag aatcccccga
    15541 aggaaccggg ggtggattcc gtacgctctg cgatcaggtc cttcatttcg gcaatgggga
    15601 cgatcttggc ggccccgtga aggcccagcc catgtcccag gttggcatcg gggtccgcgt
    15661 cgatggccaa aacgctcttg ccttcccggg caaaccattt tgccaaaaaa gccgcgaggg
    15721 tggtcttccc caccccccct tttccgctca ctgcgatctt caacgtgcaa tcctccgggc
    15781 ttctccccgc agaacgctcc acccgcaccg tgaccgatca cccttgatcg ttcgggcagt
    15841 gcggaggatc gggatccacc ctcagacgga gcgggtctgg cgggtgcgtg caattcccat
    15901 caaccgataa accagcatgc cgctcaaaac cgcgatgtcc tctgcgggaa ggccttgttc
    15961 tatggatgcg tcaaatagga tcgtcgcttc gggggagagt tcttcatccc cctcccaaag
    16021 gacgatttgg atggtgatgt tgggcaatac ccgaaaggcc atccccacat ccccgttgtc
    16081 aacgggagtc ccccccaggg cctcggcgca cgcccggaac aaagccgctt cccgcccgaa
    16141 agtggatagg aacgggtccc tggctcgcct ctgaaaggcg tcgaagtaaa aatggccgtc
    16201 gggaatctct cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccctggct
    16321 cgcctctgaa aggcgtcgaa gtaaaaatgg ccgtcgggaa tctctcggaa ggtgatgagc
    16381 tggtcggagg aaggccgccc ccgggcgcgc tccaggtagt ggcaaatgag aatcttttcg
    16441 ggaagaggca cctccctgtt cttcccctcc cggatcactt caactccgtc gttgacgcag
    16501 accaggtacg gggtgccgaa aaagagtagc cgcaaccggc aggtgccgtt ctcgaggacg
    16561 gtcagatcgg cccccgaaaa cttggccacc ctgggcaggt cggccttgac cagcctttcg
    16621 gctgccagtc ggaacgattc cctgtaatcg tcgattctcg ccacgatctt tggtgactcc
    16681 gtgaattccg cacatcggcg ttgccggcgg ggctttctag tacaacctat tataggaccc
    16741 gaaaaagcga gaaatcaacc cttacttctc ccctatttct cacggagtcc gcgcatccgc
    16801 ctcgtatcgc tctctcccga atctctcccc gtccgttgct tgaaaaaccg catcccggtg
    16861 atgaacggcc tgggttgagc gatttggccg gcacgcatcc cgtttcgtca ttcccgcaga
    16921 ccatcgggaa tccctgaaga gcgtgcatac cctctgagtg ccgggtgccg gttttcgtgg
    16981 ccaggacggg ggggatgatc ccggaatcgc tcaaatcagg tatggatgac tgcagtacag
    17041 agggggagaa accatggcca aggccgttga aaccttcatt ggacgctgcg aaaattgcgg
    17101 ggcaaaggac cgcattcccg gcccgcggct tgccgaacgc gccctgtgcg gcaaatgcgg
    17161 caagccccct ccatcctccg gatcttaccc ggaactggcc cgtctcattt cggacgcctc
    17221 cttccggaaa gaggttctgg atttttcagg ccccgtcgtc gttaactttc actcgcccac
    17281 ctgcccttat tgcaggcgtt tcgatgcgac cttccagcag ctggtttcga aatatgccgg
    17341 gaggatcaag ttcaccaaga tcgtccggga gcaaaacccc atcaccgcat cccgcttggg
    17401 ggtccaggga gtccccaccc tcctgttgtt ccgggagggc gtgctcaagg accggacggt
    17461 gggactgctc cccgaggccg acgtggaagc gcacctgatg cggattctct aaggtgcggc
    17521 ctgacgggcc cacccatgaa aaaaccccgg gccctttggg ggcgccgggg ttttcctttc
    17581 tcggcaaatc ggaatccaac ggaccctatc agcagctgag ctgcttaaag aagtcgtttc
    17641 ccttatcgtc caccaggatg aaggcgatga agtcctccac ctcgatcttc cagatggcct
    17701 ccattcccaa ctcgggatac tcgaggacct ccactttctt gatgttctct tcagccagca
    17761 gggccgctgc gccgccgatg gatcccaggt aaaagccgcc gtgcttcttg caggcatcgg
    17821 tgacctgctg ggatcgattg cccttggcga tcatgatcag ggaaccgccg ttggcctgga
    17881 aaaggtccac gtaggaatcc atacgccccg ccgtcgtcgg accgaaggaa ccggagggct
    17941 tgcccttggg ggtcttagcc ggaccggcgt agtaaatggg atggtccttg aggtactggg
    18001 gaagtccttc gcccctgtcg atgcgttcct tcaacttggc atgggcgatg tcccgaccca
    18061 caatgatggt tcccgtgagg gacaactggg tcgtcaccgg gtacttggtc agctcggcga
    18121 ggatctcctt catggggcgg ttcaggttga tcttcaccac gccgtgttcg tgtttaccgc
    18181 ggtatttttc cgggatgaac cggccgggat tgtcttccaa ctgctcgagc cagatcccgt
    18241 ccttgttgat cttcgccttg atgttccgat ccgccgaaca ggataccccc atggccaccg
    18301 gacaggaagc tccgtgccga ggcaggcgaa tcactcgaac gtccagggcg aagtacttgc
    18361 ccccgaactg ggcgccgtac ccggccttgg cggccgcctt ctcgattttc gcttccagtt
    18421 ccaggtctcg aaaggcctgg ccgtgctcat ttcccttggt gggaaggttg tccaggtagc
    18481 ctgtggtggc caacttgacc gttttcagac atgtctcggc ggaggtgccg ccgatgacaa
    18541 aggccacgtg ataaggcggg caagcggcgg ttcccagggt cttcatcttt tccacgaggt
    18601 acttttccag gttggccggg gtaagaaggg ccttggtttc ctggtacagc atggccttgt
    18661 tggcggaccc ccctcctttg gccacaaaga ggaatttgta ctccgccccg tcggtggcgt
    18721 agagatcgat ctgggccggg aggttggttc ccgagttgat ttcatcgtac atggtcaagg
    18781 gaatggtttg ggaatagcgc aggttttcct cggtgtaggt cttgtaaacc cccagggaga
    18841 gcgcctcctc gtcgtttcca cccgtccata cctgctgacc tttcttgccc acgatggtgg
    18901 ccgtgccggt gtcctggcac aagggcaatt cgcactttgc cgcgatctcg gcatttcgca
    18961 gcagggcgac ggcaacgccg cgatcgttgg gggacgcctc gggatcgttg aggatggccg
    19021 ccaccttctc caggtgggcg ggacgaagct tgaaggagac ttcccggaag gcctggttgg
    19081 caagaaagct caatcccgcg ggatccacct tcaggatctg ctggccgtcc aggctggtaa
    19141 ccgaaacata atcctttgtc agaagacggt atttggtttc gtctttggtc tggggaaagg
    19201 gctcttgata cttgaactcc ggcatggttc actccttttc tcgaagatgt ttcagattgg
    19261 atgcgtggat cgcatgaggt ccggtttcat gacgattggg cgggatcggg gaacgaaacc
    19321 cccgggtcgg catcgcatgg agagaggatc tttgggcctc gtatcgggat ccgctaagag
    19381 catcgacgtt gaccaccccg cttcggcagg caagggatcg gcgacctgcc tcccccccac
    19441 aggttgtttc ccttcggacg attcgcagca ggtctgaaga taggtttaaa ttttataata
    19501 ttatttttac tcctgtcaaa ccttttcccg ccgtaccgtg tgcaaaccgg ggggtgcgga
    19561 ccttcttgcc cgtcccccgg caggtcggca tcaattccca tcccggcgcc tgaagaattt
    19621 tctccgagga gcaggaattc ctttgtcatt ttctgaaaac tttataaaat tccgccgact
    19681 cttgatgaat catcggcgac tcggaatgat tgtccttccc cccgcccttt caaggagaca
    19741 tcgctccatg gagattcgag tcctaggctc gtccggttct gagggtcccg ggaggaaccc
    19801 aacggctttt ctcatcgatg atttcctgct cctcgatgcc ggaaccgtgt ccctctccct
    19861 ggacttagag tctcagacca agatcactca catcttcctc acccatgccc acctggatca
    19921 catcaaggcc attcccttcc tggtggacaa catcgtggca acccacggac tgggcaagat
    19981 caccatacta agcggaagag acgtgatcgc ggatctcaaa cgaaacgtgt tcaacaacaa
    20041 gatttggccc gatttcaccc tgctgcccag cagtcacaat cccatcatgc gttaccaggt
    20101 ggtctctacg aggaagcccg tcgaggtccg cgattaccgg atcttctcca cccgggtgag
    20161 tcattccgtt cccgcctacg gctacgtagt tgccgattcc agcggcgagg ccctggtcta
    20221 tacgggggac acgggtccca cggaccggct ctggaagaga atcaagggct tcaaggtcaa
    20281 ggccctcatc atcgaaacgt ccttccccga cgagatgtct cggctggccc tggattccgg
    20341 acacctcacc ccttcgctgc tccacaagga acttcgaaag ctgcccgtgc ctccggacca
    20401 tatctacatc acccatctaa aaccctatta ccgggaggaa atcgagagac aactggagaa
    20461 gctggacggg attccgttga ccgtgctttc ggacaacttg aggttttccc tgtagggctt
    20521 cctgcggcgg gacgccccgc ggcgcaggcc gggccgggga gggccggggg attcttcggg
    20581 atgcaagccc cggccggtac gggcaggggg ggaagggtgt cgcgtccttc ccgagggtga
    20641 aggtttgacg gagtgacttc tcccgccgaa gcggtccgtt atgcatcctc cccgagggcg
    20701 agggtatgac cttggatatg cacgcgaatt ccctgggagg tcatggctat tctcaaggca
    20761 ttttttgagc gcaaactcca cctgctgatc ctgctcggtc ttacggcttt cctcctgtct
    20821 ctttttccgc cccctctcct gctcagcccg accagcatcg ccggggggga cacggcttcc
    20881 catttgccct cggcggccgt tatgcggcac cggcttcttt cgggaggacc tctgtttgcc
    20941 tgggtcccgg gcaactacgc gggctttcct ctctttctaa actacttccc tctccccttt
    21001 atcctcatcg catgcctgtc gttcctggtt tccctcccca ttgcattcaa ggtggggacc
    21061 ttgctggcgg taatcccgtt gcccctggcc atgttcgggt gcctgcggaa cctgggctgc
    21121 cctcaccccg cccctgccct gggggccgcc ttttccgtgc tttttctggc catgaaagac
    21181 aattccatgt ggggaggcaa cctcaccagc accctggcgg gggaattctc gtacgggatc
    21241 tctctcatcc ttgccgtcta cctggtgggg aaactcttcc gagacgttcc gcggagaagg
    21301 tctttgatcg ccaatgccgt cctggaagct ctggtggctt tcagcagcgg atttccggtc
    21361 ctccaggcgg gcttcggcag tttctatttc ctgcgccgcc ttcgccattg gccctacctt
    21421 atcgtgctgc acgccctggc ggcgggcatg atcggcttct ggctgcttcc cctggtctgg
    21481 cggttgcctt ggaccacccc ggcccaccac acctggcact tccagggatg gtgggaggcg
    21541 gtcccggtga tcctctggcc cgccgtcttc ggcatcctcc tgggctgggg ggtgcaggct
    21601 tatgcccgcc tgaaaacacc ccggactccg ggcgatcccc aggacggccg cccccaggaa
    21661 tacttgtggt tccaggtggg cgtttctctc ttattctttg cttgcgcacc ctggatcggt
    21721 ctggcggacg tccggtttct tccctttacc caaattctct tgctcttgct gggggctatc
    21781 gggtggggcg aattcctgaa ccgatttccc tattcccgga ttacttcctg ccttctcgtc
    21841 gtcggggtcg tcctggggag tcagcccgcg ttgcgcgaaa cccgttcctg gatcgagtgg
    21901 aactactcgg gattcgaagc caaggaactt tggcccgtct tccagcagat caacagcgca
    21961 ttgaagggaa cggaaaacga tccccgggtt ttctacgagc actcggccct caacgaccgg
    22021 ctgggcaccg cgcgggcctt cgagatgctt ccctattttt cgggaaggag caccctggag
    22081 ggactctaca tgcaatcgag cctcagttcc cccttcgtct tctatcttca gtcggaactg
    22141 acccagagtc cgtcctgtcc ttttcaaggc tattcgtact cgagagtcga tgcggtccgt
    22201 gctgcccagc atctgcggct gttcaacgca aaccaggtca ttaccgtaac ccggtgcgtc
    22261 agcgacctgc ttgatcaatg tccggactat gccctgcaga ggtcgatccc accctatcga
    22321 atctacgagg tttccaattt cgagcccgcc tatgtcgttg ccgtgcggta cctgcctcat
    22381 cgaatcccgg cagcgggatg gaaagcgacc caatacgagt ggtttcgaag atcatccctg
    22441 gaggtacccc tggtcgtcac gccggagtct ccggacggtg actactggct tcagcttccc
    22501 ctttgggagg ggagcatgga gcaaatcccg atcatccccc tggcccgcgc aggcgaggtt
    22561 caagtgacct ccaccctgga aggagaccgg attcggattt ggacaagtca cctggaacat
    22621 cccctttgga tcaaggtgtc ctatcatccg gactggcatg tggcggaagg ggcaggagag
    22681 ctctacgcgg cttcgcccgc tttcctcctt ctgttccccc gcagtcaccg ggtcacccta
    22741 cagttccaac cccgcggcgg ggtctactcc atcggcacaa cgacgagcct cattgcctgg
    22801 ttggggtccc tggccctggt cggctggtcc cggtggaaag ggcgggttcc cggaacggga
    22861 gaaacgggca gctttttcaa aggaggtacg agccgagaca cccgggtttt tggtcccatt
    22921 ccgaaggccg gccttcaaga cggcaacgga aaagggtccg acgaggtcgg tcccttcctt
    22981 cccgcatggg cctgggtcct cttggggatg tccatcccgg ccgtcctgtt ttggaggggg
    23041 cacgaagacc ccattcttct gtttcaccgg ggcgtcaagg cctatgatca agcggacgcg
    23101 atgccggcgg tttccggggg tcccccctcc tccgacatca caacgggaaa agcgcgcttt
    23161 tctgctgcga gaaggcattt tgcccgatgc ctggaacggt tcccaaattc tcccgtagtg
    23221 gaccatgcct tccactactt tgttctcacg ttcatgaagg aggatcgcct gcaaacggtg
    23281 atcgatctgg tacaatcctt tctgatccgc taccccgaca gccgcttccg gccccaggcc
    23341 ctctattatt cggggctttc gagctcgggc ctccagtgga tggaaagtgc caggggcttc
    23401 tactggcagg tcctccgcca attccccgag gaccccgtcg ccccgagcgc ggcggcacgg
    23461 ctcatcgagc tcgactcccc gcaatccatg ctctccgcgg cacgggaact ctacgcccaa
    23521 gaccggcacc tgcaggctct tcccattctt gaaagccttc gcggcagccc gataccggaa
    23581 atccgctccc aggccatgct gctctccggt tactgccgct tttacctgag tcactgggag
    23641 gaggcgagcc ggttgctccc ggcatggctg caagaattcc ccggccattc ggataccgcc
    23701 acggtcctgt ctatgcttgc cgagagtcgc atggccctgg ggcaataccc ggaggccttg
    23761 gagagcctcc ggcaggccat ggctctggat ccctccatgg cccggagttt ctcgggcatg
    23821 gccctgctaa aacatctcga ggacctggtg tcgatttcgg gcaaggccat ggaaccgtga
    23881 tctttccgag gcgagacccg ggagccgtct tcgaattgtc ctacgcaaag ccgcgaaccc
    23941 gtcgagcagg cttgcgtccg ggtccgtctt ggcttatgct tcttcattcc tgaacaagac
    24001 actccacaag gttttggaac attctgaaga ggaacgggct gagatgcgga gctccaaggg
    24061 gcatcgtccg atcatgcgca atgagagtgg attactccaa ccgggcagca agtcggagcc
    24121 cctcgaggga ctcgaatgag cagtcccttg tccgacccct tcgacttcac ggtcctgatt
    24181 cccctttaca acgaagagtc ttctttgatt cccagcgttc atcggctcct ggagttcttt
    24241 gtccgacatg ggcttcgggg gcggatcatc ctggggagca acggctcgac ggaccacacg
    24301 gtcctggtgg gagagcttct cgaggacagt tatccggaga tcgtccggtt tttccatacg
    24361 gaaacgcggg gggccgtggg agacgtcttc cgcaaggctg tggacctggc atcttccccg
    24421 atcctcattt ccatggacat cgatctttcc gtggacgtgg acttcatcct cgagagcctc
    24481 gccttgctgg aatcccacga gatcgtcctg ggttccaaac aatccggtta ccagctccga
    24541 tcagtctggc gacggctggg cagcggggtc tttctcctgt gcgcccgcct gctgctccag
    24601 ttgccctaca acgattactc cctgggtgcc aaggcctatc ggttggattc cattcgtccc
    24661 tggctcgatc gtctcagcgc cgacaccaat tatgtgctgg acctggtcta ccaatgccat
    24721 catgcaagga tgcccatcgt gcttctcccc gtggcctgtg tcgattggcg aaagagccgc
    24781 ttcagtctga tccgggaggc gatcgttcgc ttttcgcacc tctttgacct ctggtttcga
    24841 catcacctgc accgaaacag gaattccccg ggcgggtgct cctagcaccg tcgtccttcg
    24901 tcaaattcag ggaacccatg ccatgagggt ttggatccat tcggacgatg ccggcatctc
    24961 cctcagctcc actcagcgaa ttctcactgc gtggaaagag ggcctcatcg acggattcag
    25021 catcctggcc aacggaccgg cggccccggt ggtctatgat cggctggcag ccgatccccg
    25081 gacaccggct cgattgtccg tccatctgaa cctttccgaa ggcccgtgct cggcaccccc
    25141 ggaaaccgta ccctctcttc tggaccggga aggaaacctc aactgcggct tcctgaccct
    25201 ttggacaaga tgggtgagcg ggtcggccga atccagaact cgatggctgg agcagatcga
    25261 acgggaatgg agggcgcaga tctttcgggt ggcacagggg gttgctcccc gccccatcat
    25321 ggccctggac gggcacaatc atctccacat gctgcccttt cttttcggca cgaccctggg
    25381 gctggcccgg gaacattcca ttccccaggt gcgcttttcc atggagccgc ggcatgttcc
    25441 ccgtcctcat ttccggctcc tctcttttcc tttctggatc aatgggttga aattcctggt
    25501 gcttcgcgtt tgttgccggg cggctcggga aagattttcc attccccgcg aattgcggac
    25561 aatccccatc gtcggcattt tgtccagcgg gcgaatgacc gcccggtccg ccgtggcggc
    25621 tctcgaaggg gcacgccgag atcggattcc cgaaatcgag tgtgtctttc acgtgggtcg
    25681 aatggaagcg ggtgaagtcg aatcctggaa atgccgactc cgcgcccgaa ggtttttttc
    25741 gagcccgtgg cggcaaaggg aatacgagga gctcagcgca ttggattggt caagccatgg
    25801 ttttcacaga ggttttcaca gctcgagacc ttgattacga ggctcactcc gacctgatga
    25861 gaagggcttt ccatgcgctc ttctcccggg cggccatctc gaacgatttc atgtcccccg
    25921 cctattaccg ttggaaatac cgtccccccg ccggagaagg tcgcacggcg gtggtgttcc
    25981 acggaaaccg tatggtggcg accagttcca tgtttcctct tcacttgaaa cacccgggcg
    26041 gcatgtcccg ggtctggcaa tcctgcgaca gcgcaaccct gcccgaggca cggggggcgg
    26101 ggtatttcac ccgctgtctc gaagccctta tcgagattct cccccccggc gagatctttt
    26161 tcggtcttcc gaatccctcc tcccgccgag gatgtgagaa ggttggctgg cggcctcagc
    26221 aagtcattcg cacctgggtg ggtttttccc ggcccggttt cggcctcccg gcccggttca
    26281 gcggtcggtt ccatgccgaa ctgggcgagc actgggatcg gttccgcccc cgagaccgtc
    26341 ccgtgctcag tcgggacccg gcttatcttc aatggcgata ccttgaaaat ccctgttttc
    26401 gctatcgctt ctttaccgac atctcccctg aggggttcgg atttgccgtg gcacgtttgg
    26461 ggagttttcg gcggatccct ttcggactgg tcctggaatg gtgggcaagg gagcctgccg
    26521 tgcagaaggc tttgttcgga caggtaagcc ggtggaccct agaacacggc gcgccgctga
    26581 tcatgactct caacagccgc cttcggaaaa ccgacgccct gcgaaacggc ctgttgccca
    26641 tgcccgaatg cgtgctgttc aaaaaccagg tactcatggg caccgccagg gacccggaag
    26701 ggcagaagct gttccgggaa aaatgggatt ttcaattggg ggattgggac gggttttgaa
    26761 ggacctctct tgttctactt gtggtccagg gtcgtgacga agctccattc ttccgagggc
    26821 ttccgttcca gcaggtgtcg gcatcggccc tggtacaggc gggaaggagg gtcaccgggc
    26881 cagaacttct cgacctcgag aaatcgcctc agggcttctt cccatcgcat ttcctggtag
    26941 aatcggatgc cgtccccgta cagccggaga ggttcccggt gggcggccgc gttctctccg
    27001 ttgtccatga gctgatgaat cgttacgggt ttttgtttcc ccttcacccg caccacgtcc
    27061 aggacccggc ccacaaagcg gtcttgagcg gcttcccacg tggactcgtt caccacgatg
    27121 gtcactccgt acatcttggt gagcccctcg agccgggacg caaggttgac gttgtctccg
    27181 agaaccgtgt agtcgaaacg gcgctcggaa cccatgtttc ccaccatcat gagccccgtg
    27241 ttgatcccca ccccgatctc gaggaccggc aggccccgct ccttccagcc ctgctgcagg
    27301 cccttgagga ctcgaatcat atccagggcc gtggcgcagg ctcggtgcgg atgatctttc
    27361 tggggcagag gagccccgaa gatcgccatg acggcgtctc cgatgtactt gtccagagtc
    27421 ccgtgatggt cgaaaaccac ctgggtcata cgggtcaggt actcattcaa ctgggggacc
    27481 agttcctcgg cgggacgctg ttccgacagg gtggtgaagc cgcggatgtc cgaaaacatc
    27541 accgtcagct ccttcttctc tccacccagg cgcagccgtt cgggattcgc cagcatctct
    27601 tccacaacgg atccgtgcac atataggcta aaggtgttgc ggatcattcg cttctctttc
    27661 tcttccctga gatagcggtg gaccgaagtt cccaggtagg cgacggccag gttcatggtc
    27721 gggtaaacgt gattgagcca gagcccgccc tgcagaaaga gccaatgggt caacaggatg
    27781 tagcctccga aaagaaacag gaccacgccc gccaggggca gtccccggat gaagaactgc
    27841 atcaggagga agaccccggc cagtccgaaa atggcggcca aatcaaccag gggcatgttc
    27901 cgttcgatac gctggaggaa gtgctgccgc gcaatattct ccatcaccgt acagtgaagc
    27961 tccacgccgg ggaaggtcat ggaaaaggga gtgggccgca tgtcgtagag acccagtgtc
    28021 gtatttccaa tcaccactat gcgatccttg aggcagtcgg gcggcgattc gtgatgcatg
    28081 agggttgtgg cggaaaagtg aggaaaggtg taggcgggtc cgtagtaatt gagaagcaat
    28141 tctcccttgt tgctcgtggg gatgctcacg gaccccagac ggatgttgcg caccccccgg
    28201 gtgtccaggg tcaccatcag cggggaattc ggaaaagcca ccgccaacat ttgcatggcc
    28261 agggagggaa agatgcgatc ctggaaacga aaaatcaacg gcatccaccg gaccgtcccg
    28321 tcgggatccg agaaaacgtt gaagctgccc atgtagcgac tggattgctc gatgacggga
    28381 atgttggctt ccaggccgac ggcctcgttg agtctaccga cgcgggggct tcccatcgtc
    28441 cgcacgatgg gaacctgggc cttttccaaa agctccgtgg gaggaggctc cgggacgaac
    28501 tggtccttgt cgaagtagaa aaagtgtccc aacaccagag ggactttgag cctttgaagg
    28561 gtatcggaca ggatgagatc gtcgtcctct tcctgggcaa tggtttccag ccagcggacg
    28621 agttccttgc ccgccccggg cggcatccct ttttcgatgc gggaacggac atccaggacg
    28681 gcatttcggc ggagtttcaa atccggctcg aaaaatccca tgtccagtcc gatgaccttg
    28741 gcaccgtggt cttgaacggc ctcaatcaag cgggccatct gccttctggg ccatggccaa
    28801 cgcccgtcgc gattgacgct ctcctcgtcg acggccacaa aggcgaagtc cccggaaggc
    28861 tccaccgtgc cccgttgtct gaaatgaagg tcccatgcct tcaattccac gaattccagg
    28921 aagggaacga ccaggaaata caaggcggtc aaaaggaggg tgctgcagaa gacgaaaaca
    28981 acctcccagg acaagaaggt ctgcagaatc ttacacttgg gcttcaaagg actgtagcct
    29041 cgtagcggac gaccgtgtat gcgaggggcg ggggaacgcc aaaatcccgc agcctagttt
    29101 gtaatggcgg accatgtatg cgaggggcgg ggactcgccc cgccctcggc acttctgcgc
    29161 aagaactggg aacagacacg gccgtcccgg ttcggctccc tacgacgggg acggtttgcc
    29221 tcgtgctcgc cggagccccc cctgccccga cgccccgacg aaagcttcgg agacctagcg
    29281 gatggtcacc accagcttgt ggcgtgtttc gcccgccgga accgtgaccc gataggcatt
    29341 cttggttcgc atgtccaccc ggcggcccac ggccgggtct ttcaacatga ccttgcgttt
    29401 ggcggaaaga ccctcgatcc cctcccacct cagcacatag gtggtcgcct cctgtcgcgc
    29461 cggaacttcc atgaccaagg ggaaacgggc cgggaatttt cctgtggacc ggaaatcccg
    29521 ggcatatttc cccttgcggg tctcccattt gcggtggtcc acatagattc tggggacgtt
    29581 ggcggacacc ggcggcggcg ccagggcatc caggaaatcc gggccggcgg aagcccgagg
    29641 atccagcccc agcaggagtg tccggtccgc gtatttccgg cgggcctcgc tgagggagaa
    29701 ggaaatgccc ttgggtcgcg gctcgtcctt gggccgcgcc ttcaccagcc tctttgccgg
    29761 gggggcgtaa gccggttcct ccggtggcaa ggctcccgtt ggaaaaatcg ccagcgccac
    29821 ctccatatta ctgtaattgt acacccaata gccgttccac ggttcgaggt tcgactggga
    29881 accggtgatc aggggagcgt aggccccggt ctccacgggg aaatacccca tgtcataacc
    29941 ataagccacc tgctggaccc agacgtttcc cgggtcgtcc agggggtagt aaatctcccc
    30001 gtcgaaaacc aggacctggg accatggcgt gctgctcagg aaaggatggg ccagcatgtt
    30061 ccagccggga taaacggtca aataatactc gcagtcctcg ggaaccgccg tgccgcctgc
    30121 cgtgatagtc cgatcgggca cggtgatgaa ccaaaagccg tagccgggca tgacccaagg
    30181 ggtcatcggg tattccaaat actctcccgt gagggggtct ttgtcataga gattgtcgta
    30241 ccatagaatt cgctggatgg acggggtgta ggaatactgg cccgtaaaga catagagagg
    30301 atcgtcctgt ccgacgggaa ggtagacgcc cgggatgccg ataagatgat agtccagctc
    30361 gtcccaaatg ccgtccccgt tcacgtcccc cttacaatag taggtggtgt agagatgggg
    30421 acactcggtc caggctcccc aggagttccc gtcgaaggtc ttcacccgca cgtagtaaac
    30481 gaccccggcg gccagggtgg gcccgccgta gggcaccgcg gttcc
//
LOCUS       DAOOVC010000037        30403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1023_length_30403_cov_6.586299, whole genome shotgun sequence.
ACCESSION   DAOOVC010000037 DAOOVC010000000
VERSION     DAOOVC010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 30403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30403
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_30403_cov_6.586299"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..1073)
                     /locus_tag="PLM79_07030"
     CDS             complement(165..1073)
                     /locus_tag="PLM79_07030"
                     /inference="COORDINATES: protein motif:HMM:NF018590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HOV86099.1"
                     /translation="METSFKAYAARELGVRIPDDYARFMEEYGRRLPEDPMRGESWVA
                     GLGNEEFVIGTTLAFRSALPNFPKSYVVMGYGGLKQIIVNHMYEDIDQFFVLDARDGK
                     VLSVDSRGVSRAVADSFEEWVSPELLRAQLRDQYTSTLTVLVFQDARKAEEARSKLLK
                     LQKEGFLVLDDAVVVVKGEDGSTRYHPMHKLVGRSLAVGSITGVIVGAILMNPLLGVL
                     FGAVTGGVSASMGDAGIDDGFVQQLAAKFQPGCSALFCLVREADADHIGEEFRGFGGK
                     ILVTSVDREKEASFQELLDAVKKDGE"
     gene            complement(1155..3671)
                     /locus_tag="PLM79_07035"
     CDS             complement(1155..3671)
                     /locus_tag="PLM79_07035"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOV86100.1"
                     /translation="MTERKLLTTSSTKGDPAKGEIRTGSSSAAIKQSILDNLHFIQGR
                     GPFNATPNDWYMALAYAARDRMMDRWVRWIEGMRSKQDKFVGYLSAEYLMGPHLGNAL
                     LNLGILDAAREALSELGLDLRDLLMQEEEPGLGNGGLGRLAACFMDSLATLGIPAIGY
                     GIRYEFGIFDQKIQDGWQVELTDKWLRLGNPWEIPRPELAYEVDFGGHTEAYHDEEGR
                     YRVRWVPHWKIKGVPYDTPVPGYGTANVNLLRLWKSEAIESFDFHAFNTGDYYSAVHE
                     KMASETISKVLYPNDEPEIGKRLRLAQQYFFVSCSLQNVLFIHQLRGLGLERLHEFVS
                     LQLNDTHPSIAVAELMRLLVDVHLMDWDTAWHVTKNTFGYTNHTLLPEALEKWHLGLF
                     GNMLPRHLEIIFEINRRFLDEIRERFPGDEGRVARLSLIEEGGGKYVRMAHLATVGSH
                     TVNGVAALHSELLKSTVLKDFYELSPEKFTNVTNGVTPRRWMLLYNQPLARLISRRIG
                     DGWIRRLEQELQKLEPWADDPEFRDEWRKVKHRNKVQLAAVVKERTGIEVSPDSLYDI
                     QVKRIHEYKRQHLNVLHILALYNRIKRNPALEVTPCTFLFGGKAAPGYFMAKLIIKLI
                     HAVGEVVNNDPDVGDRLKVVFFPDFNVKNSQWIYPAADLSEQISTAGKEASGTGNMKF
                     SLNGALTIGTLDGANVEIREAVGEENFFLFGLTVEEANALRDRGYRPWEYLGSNPDLQ
                     EVLDQIGGGFLSPGDKNLFKPLAETLFHSDPYLLLADFQSYADCRDRVGISYRDPEEW
                     TRKSILNVARMGRFSSDRSIRDYCQKIWKTKPVSNPGAEK"
     gene            complement(4011..4742)
                     /locus_tag="PLM79_07040"
     CDS             complement(4011..4742)
                     /locus_tag="PLM79_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOV86101.1"
                     /translation="MQLSLRGISQTFGTQRVLDEIGLEISDFHALVVIGPSGGGKSTL
                     LRILAGLLVPDRGEMEINGRPVVFSEERLREYRRNLGTVFQSFNLFPHLTALENVTLP
                     LEKVHGYGRKEAVAHAREVLARFRLEEHAFKRPGELSGGQQQRVAIARAVAVKPRFLL
                     MDEPTSALDPEMTSEVLEVIETLRAEGRDLILVTHHMGFARHIGDHCVFLADGRIRAS
                     GPPGDLFLHPSDPLLQRFLSRVLRY"
     gene            complement(4732..5532)
                     /locus_tag="PLM79_07045"
     CDS             complement(4732..5532)
                     /locus_tag="PLM79_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOV86102.1"
                     /translation="MKTLLLSLFFTRPTRWGETIPPSARIASGSAVFLSLALLFYFAF
                     SRLEYGWGWSAVFAYREKFLRGWLTTVAISTAALVLSLAVGLAAALARRSGFLPARYL
                     ARLFTETLRGTPLLVQILVFFYVVADAFGVGNRYVVGTLTLSLFAGAYIGEILRAGIE
                     NVSESQWDSARAVGLTPSQIYRYIVFPQVVRRSLPPLAGQFASLIKDSSLLSVIAVSE
                     FTLSAQEVNAYTFSTLESYLPLALGYLVLTLPISLWSRTLEKRFHYAT"
     gene            complement(5549..6373)
                     /locus_tag="PLM79_07050"
     CDS             complement(5549..6373)
                     /locus_tag="PLM79_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOV86103.1"
                     /translation="MKRLLRDAARQGAVLGILLFCLAGGGLIRTSGAQPMEKETLVIG
                     MELSYPPFEMTDEKGNPAGISVELAYALAKALGKKIVVENIAFDGLIPALKTGKIDLI
                     LSSMTATEERMQSIDFSDPYLKTGLCLLVRRDSPVQSVADLDGPGRVVAVKKGTTGHA
                     YAAGNLPRAKVLVLDKEAAAVLEVIQGKADAFIYDQMSVYRHWQRNRNATRAILTPFR
                     QESWAIGLRKGNEELKAQINRFLREFRAKGGFDDLGDRFLREPKEEFKRQGIPFYF"
     gene            complement(6370..7722)
                     /locus_tag="PLM79_07055"
     CDS             complement(6370..7722)
                     /locus_tag="PLM79_07055"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOV86104.1"
                     /translation="MTTHSISAKAINPAVGAGLPVALSLAASPYLLFHSLVELFSIVI
                     AFGMFARAWNSRRFFDNNCLLFLGIAYPFVGALDLVHTLAYKGMGVFPRTDTNLPTQR
                     WVAARFTESVSLLPAPLFFRRKIDVSPLLMGHTLACSLLLPGILHWEVFPACFAEGSG
                     LTVFKKASEYGICLIFPASVFLGARHRDRFDEGVFRLVVWSIGLTIASEPAFTFYIHV
                     HGFSNWVGHILKIASFSLLYKAVIETGLARPYDLLFRELKKSEEVLRESPARYRGLFN
                     SMTDGFVYGEILCGADGRPCDCRALEVNPSFEKHTGLKAEEVVGRTLSEVLPDTEPFW
                     IEPCSRAGTGGGLPGPPGPNRLHGHPGPRDRVRYPGGPCRQHREERAGTDRHAGAREA
                     FGRLLFQGIPGGRRDHHPYLVALGGARPRKPAHPRPQGAGRGQFLLRASRGFRGGREP
                     "
     gene            complement(7816..9102)
                     /locus_tag="PLM79_07060"
     CDS             complement(7816..9102)
                     /locus_tag="PLM79_07060"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOV86105.1"
                     /translation="MTETEFDFTIARLGECRIPSPMSCAHFVPEDDRVLYHRDPRTVM
                     KFLEGGKVPPSFEKAGPREKIYFDPTKLKCGIVTCGGLCPGLNDVIRSVVMSLFHHYG
                     VRNLFGFLYGYEGLSPRFRHAPLELTPQSVSRIHEQGGTILGSSRGPQDVSEMVDTLE
                     RMNVGILFTLGGDGTLRGAQAISREIAERRLKISVIGVPKTIDNDISFVQRSFGFETA
                     VSEAGDAILSAHTEATGARNGVGLVKLMGRESGFIPAFAALAYNDVNYCLVPEIPFTL
                     EGFLQNLEERLERKGHAVILVGEGAGQDLLQGTQARDASGNVRLGDIGAFLRDRINAH
                     FKERGKEVNLKYIDPSYTIRSVAANAHDSAYCLLLGQNAVHAGMAGLTNMVVGDWGGE
                     FTHVPIPMAVSSRKKLDPQGWVWSTVLASTGQPAVM"
     gene            9355..10788
                     /locus_tag="PLM79_07065"
     CDS             9355..10788
                     /locus_tag="PLM79_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86106.1"
                     /translation="MRWSMPFPLNIRQKVMVGLTVILLVVISIGTLSYRYLREIERKQ
                     HFVEVADDLSNIILEMRRYEKNYLLYGSREDVEENRRYVLQGKEVLKKMTPEAREFQA
                     GPLLIRLEQELWGYGALMQRTVQWAEGDKTKPDPRWEEQLREHGKSLVDLSQEMVRFE
                     RRRILKIIQTLKTQLLASLAMSALLGVFLIPVVAHKIIRPLRTIENTTHRIARGEFSP
                     LPVPETPDETQQVVQAFNRMVVELEKHQDQLVQAKKLSSLGTLTSGIAHQLNNPLNNI
                     STSCQILLEELNHPDSELMRRMLGNIEQEVHRARDIVRGLLEFSRMREFCLAPTSLPE
                     VVERSIGLISSQIPPRIEVVADIPPDLSLSLDAQRMQQVFLNLIENAIQAMEPPGQIR
                     ITAGRVPEEGAVLISVADTGKGIPEGLLGRIFDPFFTTKEVGVGTGLGLSVVYGIIQK
                     HGGTISVESKPGEGSRFIIRLPAETVC"
     gene            10805..12154
                     /locus_tag="PLM79_07070"
     CDS             10805..12154
                     /locus_tag="PLM79_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86107.1"
                     /translation="MRGPRILIADDERIARENLEHVLRKEGFDTTCVENGESAIRELE
                     REEFDLVMTDLCMQPVDGMQVLERARELYPDTEVIVITGYATVNSAVEAMQKGAYHYL
                     PKPYKIEEVRALVRKALEKRSLRQEVTALRRQIRDLGESSLLIGKSAEMEALKRTIEQ
                     VAPTDCNVLILGETGTGKELAARMIHRLSPRRDNRFLAVNCGAFNEELLANELFGHDR
                     EAFTGARGIKKGLLEAAEGGTVLLDEIGDMPPPMQVKLLRVLQERKFLRVGGTEEIPV
                     DIRVLAATNKDLKHEVEMGSFRRDLFYRINVITLHVPRLADRKDDILLLSFHFLEKIS
                     RAQGKKIEAMSDRVTEILLSYEFPGNVRELENILERAVTLARGTTIEVEHLPSDLRRP
                     GLRVHGPRRGYLSLEENEREYIAWILREVKDNKTRAAEVLGIDRVSLWRKLKRYRME"
     gene            complement(12197..13261)
                     /locus_tag="PLM79_07075"
     CDS             complement(12197..13261)
                     /locus_tag="PLM79_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV86108.1"
                     /translation="MVPVNEAIQFIHLDPLSVGFLFLVGLIGGLVSGFIGSGGAFVLT
                     PGMMSLGVPGTIAVASNMCHKFPKALVGACKRFKYGQVDIKLGLVLAASAGVGVQAGI
                     RIQHFILDRWGPAGSDLYVSLSFVSILLVVGGYVSRDAWKTWKSGGKEQVSALALKLQ
                     KIHLPPMIHFKTANVRISLWFTVPVGFATGVLAATIAVGGFVGVPGMIYAMGVSSLVS
                     TATELVIAFVMGLAGTINWAAYGMVDIRLTLIILAGSILGVQLGAIGTTYVKEHTIKT
                     VMATIMLIVAFSRALAVPIYLHRLGLVSLDSGILGVLDRASFVTMCLALGTGAVIIVG
                     SMILGWKSAHTTLEPGYAEA"
     gene            complement(13321..14106)
                     /locus_tag="PLM79_07080"
     CDS             complement(13321..14106)
                     /locus_tag="PLM79_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86109.1"
                     /translation="MTKDDPTNQSAGKGSTERRKILVVGTGDAFSEETVSYAVHLAER
                     LDYDLVALSVDESGRRGYPDRWAAKAAGTLKRRAAQKGIRCEHAVKAGDPGWAVDELQ
                     HEIRRIEFVVTDLGVNREEVAREVGIPMFSVISGSMDSKGGTDMTKEQGVPRKRPVAR
                     TLAYGLLTAAMYGAVFWNADAVMGTFTRGGWYAAFPVGTVFLFSFAHGAFAANLWSLL
                     GIEARKKPVLRETEPKASRKKKSAQKRPRAYAYVNPFHRIDKA"
     gene            14387..16414
                     /locus_tag="PLM79_07085"
     CDS             14387..16414
                     /locus_tag="PLM79_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86110.1"
                     /translation="MTFFRKLRGQEEETEDASRDLQELREALERARLPEAVGGVAAKE
                     LERIRRTDSSTPEYTIGVNYLEFLLALPWNRFTEDNLDLGRGERILEAQHYGLRHVKE
                     RILEYLAVRALCSLRDFHILVVDDEPIARTNLEYVLRKEGYRVSTAANGVEALEMVGK
                     REFELILTDLKMEKMDGMQLLESVKHVAPGTEIVMITGYATVSSAVDALKKGAVHYLP
                     KPIDLNELRSTVRQIREKKRFFQVTRSPVLCFSGPPGTGKTSIGRSIAEALQRKFIRF
                     SLAGVRDEAELRGHRRTYVGAMAGRVLNEMKRVGVKNPVFMLDEIDKMGRDFRGDPSS
                     VLLEILDPEQNGRFMDHYLDVPFDLSAVLFIATANLVEGLPEALVDRLEIIPFHGYTE
                     REKNEIAHRYLIPRQMREHGLTDRGVEFAAGAVGKVIRDYTLEAGLRNLEREMATICR
                     KLARVILLDRETPAPLEVDETLVEKLLGPPRYRHEIAEEGFRLGVTTGLVWSELGGEI
                     ISVEAGLMKGSGQLILTGSLGSVLRESAQTALSYIRSHARELCVDPEFFRGHDVHIHI
                     PAGAVPKDGPSAGITIVLALASLLTGRPARRDAALSGEITLRGRILPVAGIREKVLAA
                     QRAGIKVVVFPRRNRLDVDNLEEDVKNGISVILVDEIPELVDVVLQKTPKP"
     assembly_gap    16574..16673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16694..19003)
                     /locus_tag="PLM79_07090"
     CDS             complement(16694..19003)
                     /locus_tag="PLM79_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86111.1"
                     /translation="MKLKYKINFVSLGILTAVTCAVALVGILTINQVVIDLNRKLMSM
                     EVHNLIATIRSAHQVLKEAGVGHVESYVRKAQEDLIAEFRNYRFGSTGKLAIADRRTA
                     TLLLDPLEGNKGIRPSPLHRMIREGRGSMERLGGEDARFYCFDTYPDWSWTVILSVTS
                     EEVLEARTKFLESAGIILLPGLILGSLLFLWFTDRLVKPIHQLATAAASVSRGEWDVP
                     LPNPRGEDEISQLAAAFREMSAKLSATYRNLQENLNKVERSQEALAAEKELLAVTLRS
                     IADGVITTDVNGKVALMNKVAEAMTGWSQEEGVGRSLGEVFEILDESTGERWEHPVEE
                     VIRKGEIIDQPDHTVLVARNGRKGMVALSGAPILDQGGEILGMVLVFRDITEKTRMEE
                     EFFKAQKLESVGLLAGGIAHDFNNILTAIMGNISLAMMDAKPDAKISRRLEETLKASL
                     RAKDLTQQLLTFSKGGTPIKKIHSISDLIRSSTLFALTGSNVRCEFSIAPDLWPAEVD
                     EGQIGQVIHNLVLNADQAMPEGGLIKVRAENLAVSSTHGLPLEPGNYVKISIRDEGTG
                     ISREYLPKIFDPYFTTKHRGNGLGLAVVHSIVAKHGGLITVESELGSGTTFHIYLEAS
                     DREIAEERHPQRQLPTGRGRILLMDDEEIVGEVVGEMLGQLGYRVVLARNGEQALERY
                     RESLSSRELFDAVILDLTIPGGMGGKETVVRLREMDPDVRAVVSSGYSNDPIMGDFGK
                     YGFSGVISKPYRLQELARVLNDVTTARTT"
     gene            complement(19056..>20953)
                     /locus_tag="PLM79_07095"
     CDS             complement(19056..>20953)
                     /locus_tag="PLM79_07095"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOV86112.1"
                     /translation="LPVKEAREEDLPGRYSGKKVLWVDSYHPGYEWSDGIQKGILDVL
                     DKTGVDLRIFRMDTKNNDSVGFGEEAGVKARAVVEEYKPDVVIATDDNAQNYLVVPYL
                     RDSELPVVFAGVNMDASMYGYPCGNVTGMIEVELVEEMLRHMKQYASGDRVGYLSGNT
                     QTEREQSRIWNEKFLDGKMKTYFAEDFQGFKDQFLRAQQEVDMLFLVNYAGIRDWDPI
                     AAEKFLARNTRVPTAASVQYMDRFVVFVMGKYPEEQGEYAAQTALKILGGAKPSDFPL
                     TANRRAKLTVNLKMAQAAGIVLPVSVLKIAHVLGKEAHAEGNAVPEVSEPGRYRGKRI
                     LWVDSYHGEYPWSEGIGRGIRQVLVDSGVELHTLHMDTKRHDSVEFGKEAGRRARAAV
                     EEFKPDVVIATDDNAQKYLVVPYLKDTDLPVVFSGVNWDASMYGYPCKNVTGMVEVDP
                     MDEMMNLFKGHAKGGRIGYLSGDVETERKIVDYYNKTFFNGWMKVYLVKTFQDFQREF
                     LRAQSETDMLFFFNYTGIEPWDPVAAEEFLARNTRIPTGSPNPFMAPFVMFVAAKLPE
                     EQGSYAAQTALKILDGTPPADIPVTVNKQSKVTVNLKMAKAAGMVLPISTLRNVVVIG
                     QEALDRK"
     assembly_gap    20954..21053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21444..22955)
                     /locus_tag="PLM79_07100"
     CDS             complement(21444..22955)
                     /locus_tag="PLM79_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86113.1"
                     /translation="MEDSRHRIEAETLRRTVKELESVLDSVSDAFFSLDEDLVLTYFN
                     AAAQRMLHRQSSEVLGRHLFDAFPEGRGSIFDEKYRLAVREKIPLAFETHFDIEPFEG
                     WYDVRVFPRDRGICVCFQRITDRKRAEETLRESEERYRSIFHHSAEAIFLTRPDGTIL
                     NANPAACQMFGYTEEELIRVGRSGLLDPADPRVAGAIEERARSGIFQGELTCIRKDGT
                     RFPGELTSLIFEGGTGDPRTSTILRDMSERRQAEEDRGRWERRLQQVEKAESLGRMAG
                     AVAHHFNNLLQVVMGNLDLALNNLPEDLRSRSNVVAAMQASRRAAEIGRLLLVYLGRD
                     PRKMGPIALCAACREVLSSRGASLPGSARILTEFPPRDPVVLGDAWQMGQVFTHLLTN
                     AAESLDRQSGEVTVRILEVPSAEVAASRFFPPDWTPRDENYACIEVSDTGCGMDSSAM
                     GRVFDPFYTTKFTGRGLGLAVVAGIVRTHEGAVTVESKPGRGTVFRVYLPVMS"
     gene            complement(23285..23812)
                     /locus_tag="PLM79_07105"
     CDS             complement(23285..23812)
                     /locus_tag="PLM79_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86114.1"
                     /translation="MRKSRRRVGRGVCLAVVVLVLSAGVASGRIRTLPIPELIAQSDF
                     IVVGRVGEVKKTGEVPKGGGRAEETLENTVLPKEVLKGRWPKGKPMHFTTRRLVHKGK
                     RVWREDELTFPETGSGVLVFVKRDPEGRLRIVNGIQGLWPMGKDGRPMGMGYGYTVEG
                     LKREIAGSLPGGGLR"
     gene            complement(24341..25885)
                     /locus_tag="PLM79_07110"
     CDS             complement(24341..25885)
                     /locus_tag="PLM79_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86115.1"
                     /translation="MANKPNITPEQRELMSGKHIGQSTDFKKMPRIDEWRNLVTTALT
                     GLSCFRESRKKPRLLESDPGFGKSKSSSSSTQGSRSLDDFDLQKFASKSFEISHLESK
                     MIGPHLTDPDLLGAAIERYNTASLTHSSKDKLDNSILGSLLVDIYSLTGTFMRRVLRD
                     EQRSDRDCQFFAIYRGVHFAAADELGRIYEVPGDEIDILLQRQMIGLTPHGKEVDALQ
                     KGTKALVYLTDAEALAYRIHIEGGKLYYRDTVTNALTAFDTTKKEWTKAGGNSCHLGC
                     ELNYDKSKKEKPKGVSGFCMTLNRSLYAFRHSLKDQPGGAFYHSSYTGGSDVICTGCL
                     TVVNGVLTYLNNFSGHYRPTPTQLQLVVDHLRAFGVPLANVKVQLLDPNDIAHSPVMS
                     ATDFLKNQDHREAQFEFDVRGPVTPLLTRKRIEEKHREHLRAEAVRTAVANYIERSGK
                     WYARASEKSKETAQRLSTVVNEEDLLQEVRFCLGLSPFSPFKTPADKRLSDGELKSRL
                     LEALKR"
     gene            26991..27944
                     /locus_tag="PLM79_07115"
     CDS             26991..27944
                     /locus_tag="PLM79_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86116.1"
                     /translation="MHRFQNILYACTGRGDETQGLQQALSLARHHRAALRVFLIHSPL
                     PKGLEAYRESFHAFISRQIDAAVQGARATLNLGEAEVPVTVDLESSDTPPAIGVVRQV
                     LVRGHDLVIKEAEPQEGGRGFNSTDMTLLRKCPCPVWLARPIRRAQNEIRVAVAVSPE
                     SRDENESYLSIKLLRIGRSLADTFSGELDVISCSDHSFEEFLRRNTRVEIPEESILSA
                     ALKLQSRHDAELRRLIEDSGIDGPYRIRYLKGHPETVIPNFVETHAVDILVMGTVARS
                     GIVGFLIGNTAENVMGRLGCTLLALKPDAYVSPLAELPLKP"
     gene            complement(27905..28387)
                     /locus_tag="PLM79_07120"
     CDS             complement(27905..28387)
                     /locus_tag="PLM79_07120"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOV86117.1"
                     /translation="MGEFSFILGTVALILGVLSESGRHALGAASILSIALDPYIYRWA
                     RRMATPRTAGPLPAIGRGNAPIHPNRCILVGYGPVGRIVHQLLRERGARITVIDLNLD
                     TVRRLAAEGHAALYGDVVAAIFPGTLALQGGGVLRNTRRHPAHIPAHGLSGSSARGET
                     "
     gene            complement(28584..30125)
                     /locus_tag="PLM79_07125"
     CDS             complement(28584..30125)
                     /locus_tag="PLM79_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOV86118.1"
                     /translation="MAQPMLIAKSSKKVEFCLIPSMANRHGLIAGATGTGKTVTLQRI
                     AERLSDNGIPVFLADVKGDLAGISQKGDDNPKILERVRLLGLSDHRFREFPVAFWDVL
                     GKSGHPVRATVSDLGPLLMGRLLNLNEVQTGVLALVFKIADDGGLLLLDLKDIRSMVQ
                     YVGDNAREFTTQYGNISKASVGAIQRGLIELEAQGGDQFFGEPMLDILDLIQTHSDGR
                     GIINILAADQLIARPKVYATLLLWLLAELFERLPEVGDPEKPQTVFFFDEAHLLFEDA
                     PPALLDKIEQVVRLIRSKGVGVYFVTQNPKDIPEKVLAQLGNRVQHALRAFTPQEKKA
                     VRIMAQTFRPNPKLAVEDAITELGVGEALVSFLEEDGTPAVVQRAFVCPPESRVGVIA
                     PEERRDRIRDSIHFGNYETMVDRESAFEILRNRTDRRMARDRREAQPPPAYGHPEPRC
                     QEPPRTYRQPEPRYEEPPSRRSPGRPRESMAEAFAKSALRSIGSSIGRQIIRGVLGSI
                     LGGRR"
BASE COUNT         5817 a         9103 c         9238 g         6045 t
ORIGIN      
        1 ccttcacgcc ccttcgggcg tccctcgcgc tccgcgccca tcaaaggcgt tcgagcaggc
       61 ccggccctgc gggcggcttc gctccccttg gtcgatcggc gcccaatatt gccggcgaaa
      121 tctccgcccc cgaccgacgt cggctcaaaa gcggcgttcg ggtcctactc cccgtccttt
      181 ttcaccgcat cgagcagctc ctggaaggac gcctccttct cccggtccac ggacgtcacg
      241 aggattttcc cgccgaaccc ccggaattcc tccccgatat ggtccgcatc ggcctcccgc
      301 accaggcaaa agagggcgga acatcccggc tggaatttgg cggccaactg ctgcacgaac
      361 ccgtcatcga tcccggcgtc gcccatggat gccgataccc cgccggtaac cgctccgaag
      421 aggaccccca gcagtggatt cattagaatg gcccccacga tgacgcccgt gatgctgccc
      481 acggcaaggc ttcttcccac gagtttgtgc atggggtgat accgggtgct cccgtcttcc
      541 cctttcacca cgaccacggc gtcgtccagc accaggaacc cctccttttg gagcttcagg
      601 agctttgaac gggcctcttc ggcctttcgg gcgtcctgga acaccaatac cgtcagggtg
      661 ctggtgtact ggtctcgaag ctgggccctc agcagttcgg gagataccca ttcctcgaag
      721 ctgtcggcaa cggcccggga aaccccccgg gaatccaccg aaagaacctt cccgtcccgg
      781 gcgtccagga cgaaaaactg gtcgatgtct tcgtacatgt ggttgacgat gatctgtttg
      841 aggcccccgt accccatcac cacgtacgat ttggggaaat tgggcaaagc ggatcggaac
      901 gccaacgtgg tgccgatgac gaactcttcg ttcccgaggc ccgcaaccca gctttccccc
      961 cgcatggggt cttcggggag cctccggccg tattcctcca tgaaccgggc ataatcgtcg
     1021 ggaattcgaa cccccagttc ccgcgcggca taggccttaa aactcgtttc catggagcgc
     1081 ctcccggtag tcccgtcccg gcctgatctg cgccggggat cgaggggtag gatgagcggg
     1141 gcgctgcagc tttctcattt ttcagccccg gggttcgaaa ccggcttggt cttccagatt
     1201 ttttggcaat agtcccgtat ggaacggtcc gaagagaagc gtcccattcg agccacgttg
     1261 aggatcgact ttcgggtcca ttcttccggg tcccggtagg agattcccac ccggtcccgg
     1321 cagtccgcgt acgactgaaa atcggccagg agcaggtaag gatcgctgtg gaacagggtc
     1381 tccgccaggg gcttgaaaag attcttgtcg ccgggcgaca ggaatccgcc cccgatttga
     1441 tccagtacct cctgcaagtc cgggttggag ccgagatatt cccagggtcg atatcctcgg
     1501 tccctcaggg cgtttgcttc ctccaccgtg aggccgaaaa gaaagaagtt ctcttctccc
     1561 acggcttccc ggatttccac gttcgccccg tccagggtcc cgatggtgag tgctccgttc
     1621 agggagaatt tcatgtttcc cgttcccgag gcttccttgc cggcggtgga gatctgttcg
     1681 gagagatcgg ccgcaggata gatccactgg ctgttcttca cgttgaagtc ggggaagaag
     1741 accaccttca gtcgatcccc cacgtccggg tcgttgttga ccacctcgcc caccgcatgg
     1801 atcagcttga tgatgagctt ggccatgaaa taccccggtg cggctttgcc cccgaaaagg
     1861 aaggtgcacg gagtgacttc cagggcgggg tttcgtttga tgcgattgta gagagcaaga
     1921 atgtgaagga cgttgaggtg ctgccgcttg tactcgtgga ttcgtttcac ctggatgtcg
     1981 taaagggagt cgggacttac ctcgatgccg gttctctcct tgactacggc ggcgagttgc
     2041 accttgttcc ggtgcttgac cttcctccat tcgtcccgaa actccggatc gtccgcccag
     2101 ggttccagct tttgaagctc ttgctcgagg cggcgaatcc atccgtcgcc gattcgacga
     2161 ctgatcagcc gggcaagggg ctggttgtac aaaagcatcc agcgccgggg agtgaccccg
     2221 ttggtcacgt tggtaaactt ttccggggaa agctcgtaga agtccttcaa gaccgtgctc
     2281 ttgagaagct cggaatggag ggcggccact ccgttgacag tgtggcttcc gaccgtggcc
     2341 aggtgggcca tgcgcacgta cttgcccccg ccctcttcga tgagggacag ccgggcaacc
     2401 cggccttcat ccccgggaaa acgttcccgg atttcatcga gaaaacgccg gttgatctcg
     2461 aagatgattt ccagatgtcg gggcagcatg ttgccgaaaa gccccaggtg ccatttctcg
     2521 agggcttcgg gcagcagggt gtggttggtg tagccgaagg tgttcttcgt gacgtgccag
     2581 gccgtatccc agtccatgag gtgtacatcc accaggagcc tcatgagttc ggcaacggcg
     2641 atggacggat gggtgtcgtt cagctggagg gaaacgaact cgtgcaggcg ttccagtccc
     2701 aacccccgga gctgatgaat gaaaaggacg ttctgcaggg agcaggagac gaagaaatac
     2761 tgctgggcaa ggcgcaggcg ctttccgatc tccggttcgt cgttgggata caacaccttg
     2821 ctgatggttt ccgaggccat cttttcatgg acggcactgt agtagtcccc ggtgttgaag
     2881 gcatggaaat cgaaggactc tatggcttcg gacttccaaa gccgcagcag gttgacgttg
     2941 gcggttccgt atccgggaac gggagtatcg taggggaccc ctttgatctt ccagtggggg
     3001 acccaccgga cgcgatatcg tccctcctcg tcgtgatagg cttcggtgtg gcccccgaag
     3061 tccacttcat aggcaagctc gggacgggga atttcccagg gattgcccag ccggagccac
     3121 ttgtcggtga gttccacctg ccatccgtcc tggatcttct ggtcaaaaat gccgaattcg
     3181 taacgaattc cgtacccgat ggcggggatc cccagggttg ccagcgaatc catgaaacat
     3241 gcggccagtc gacccaaccc cccgtttccc aggcccggtt cctcctcctg catcaggagg
     3301 tcccggaggt cgagtcccag ctcggacaac gcttcccgcg cagcatcgag gattcccagg
     3361 ttcaggagag cgttccccag gtgaggtccc atgagatatt ccgccgagag gtagcccacg
     3421 aacttgtcct gcttgctcct catcccctcg atccagcgca cccagcggtc catcatgcgg
     3481 tcgcgggccg cataggccag ggccatgtac cagtcgttgg gagtggcgtt gaagggaccc
     3541 cgtccctgga tgaaatggag gttgtccaga atggattgct tgatggcggc ggagctgctt
     3601 cccgtgcgga tttcgccttt tgcgggatcg cccttggtcg atgaggttgt gaggagcttt
     3661 ctctcagtca tggaacatcc ttccctggga gtcgattcaa gaggaacggg ggtccgtccc
     3721 aaaaacggcg cgttcctcct tatcgctcgt accggcgcaa accggaatgc agaaaaaagc
     3781 ggcgggtccc cggcacccgc cccggagatg atccgggttc gcccggcagg accgttgttg
     3841 gcacgcgttg ttgggtggag cggcaagtgg gccggttcgg ggcggctcgc tgccggccgt
     3901 tggaaacaac gcaaaggata ccatgaaatg ccccacgccg cgaggcccac tttcccggga
     3961 gaccttgctc gcgcggcccg gcgccgcggc acatcttcct cgaaagggcc ttagtagcgc
     4021 aggacccggg agaggaacct ttgaagaagg gggtccgagg gatggaggaa aaggtccccg
     4081 ggcgggcccg atgcccggat tcttccatcg gccaggaaca cgcaatggtc cccgatgtgt
     4141 cgtgcaaacc ccatgtggtg ggtgaccagg atcaggtctc ttccttctgc tcgaagagtt
     4201 tcgatgacct cgaggacctc cgaggtcatc tcggggtcca gggcagaggt gggctcatcc
     4261 atcagcagga acctcggttt cacggcgacg gcccgggcaa tggccacccg ttgctgctgg
     4321 cccccggaaa gttccccggg cctcttgaac gcgtgttcct ccaggcgaaa acgggccagg
     4381 acctcccggg catgggccac ggcctctttg cggccgtatc cgtggacctt ttccagggga
     4441 agggtgacgt tctccagggc cgtgaggtgg gggaacaggt tgaacgactg aaagacggtc
     4501 cccaggttgc gccggtactc ccggagcctt tcctcggaaa acacgacggg cctgccgttg
     4561 atttccatct ccccccggtc cggcaccaga aggcccgcca ggattcgcag cagggtggac
     4621 tttcccccgc ccgaggggcc gatgacgacc agggcgtgaa aatcggagat ctccaggccg
     4681 atctcatcga ggaccctctg ggttccgaac gtttgggaaa ttccgcgcag gctaagttgc
     4741 atagtggaat ctcttctcga gagttcggct ccagagcgag atgggcaaag tcagcaccag
     4801 gtagcccaag gccaggggca ggtagctctc cagggtgctg aaggtatagg cattgacctc
     4861 ctgggcgctc agggtgaatt cgctcacggc gatgacggaa aggagggaag aatccttgat
     4921 gagggaggcg aactgcccgg ccaggggcgg aaggcttcgg cgcaccacct gggggaagac
     4981 gatgtagcga tagatctgac tcggggtgag tccgacggcc cgggccgagt cccactggga
     5041 ttcgctcacg ttctcgatgc ccgcgcgaag gatctcgccg atgtaagccc cggcgaagag
     5101 tgacagggtg agagtcccca ccacgtagcg attgcccacg ccgaaagcgt ccgccaccac
     5161 gtagaaaaag accaggatct ggacaagcag gggagtccct cgaagggttt ccgtaaagag
     5221 cctggccagg taccttgcgg gcaagaatcc ggaccggcgg gccagggctg cggccagccc
     5281 taccgcgagg ctcaaaacga gggcggccgt cgagatcgcg acggtcgtga gccagccccg
     5341 gagaaacttt tcccggtagg cgaaaacggc gctccagccc caaccgtact cgagcctgct
     5401 gaaggcaaaa tagaaaagca gggcaagact gaggaagacg gccgaaccac tggcgatccg
     5461 ggccgatggg gggatcgtct ccccccatcg ggtcggccgg gtgaaaaaca aggaaagaag
     5521 aagggtcttc atggggcggc catgggggtc aaaaatagaa tggaattccc tgtcgtttga
     5581 actcttcctt gggttccctc aggaagcggt cccccaggtc gtcaaagcct cccttggccc
     5641 gaaactccct gagaaaccgg ttgatctgcg ccttgagttc ctcgttgccc ttgcgaaggc
     5701 cgatggccca ggattcctgc cggaaggggg tcaagatcgc ccgcgtcgca ttccgattcc
     5761 tctgccagtg cctgtatacg gacatttgat cgtagatgaa agcatcggcc ttaccctgga
     5821 tcacctcgag gacggccgcg gcttccttgt cgagtaccag gaccttggcc cggggcaggt
     5881 tcccggcggc atacgcatgg ccggtggttc ccttcttgac ggcaacgact cttcccggcc
     5941 catccagatc ggcgacggat tgcacggggg aatcccggcg caccagcagg cagaggccgg
     6001 tcttgagata agggtccgag aagtcgatgg actgcatccg ctcctcggtg gccgtcatgg
     6061 aagacaggat caggtcgatc ttgccggtct tgagcgctgg gatgagcccg tcgaaggcga
     6121 tgttttcaac gacgatcttc ttgcccaggg cctttgccag ggcgtaagcc agttccacgc
     6181 tgattcccgc cgggtttcct ttctcgtccg tcatttcgaa gggcgggtag gagagctcca
     6241 ttccgatcac cagggtttct ttctccatgg gttgcgcccc ggaggtccgg atcaagcctc
     6301 ccccggccag gcagaacaga agaatcccca acaccgcgcc ctgccgggct gcgtcccgca
     6361 aaagcctctt catggttccc gtcctcctcg gaagccgcgg ctcgccctca ggagaaattg
     6421 ccctcgtccg gctccttgtg gtcttggatg tgcgggtttt cgaggtcggg ctcctcccag
     6481 ggctacgaga tacggatggt ggtcccttct cccacccggg attccttgga aaaggagcct
     6541 cccgaaagct tcacgcgctc ccgcatgccg gtcagtccca gccctttctt ctcgatgctg
     6601 ccggcaaggg ccgccgggat atcgaacccg atcccgtggt cccggatgac catggagacg
     6661 gtttggtcca ggcggtccag ggagacctcc acccgtcccc gcccggctgc atggctcgat
     6721 ccaaaacggc tccgtgtccg ggaggacctc gctgagggtt cgccccacga cctcctcggc
     6781 cttgagaccc gtgtgttttt cgaagctggg gttgacctcc agggcacgac agtcacaggg
     6841 ccgaccgtcg gcaccacaca ggatctcgcc gtagacgaat ccgtcggtca tggaattgaa
     6901 aagaccgcga taccgcgccg ggctttcccg gagcacctcc tcgctcttct tcagctctcg
     6961 aaacaggagg tcgtagggtc tggccagccc cgtttcgatg acggctttgt acaagaggga
     7021 aaaggatgcg atcttgagga tgtgtccgac ccagttggag aaaccgtgga cgtggatgta
     7081 gaaggtgaag gccggctcgg aggcgatggt caggccgatg gaccacacca ccaggcggaa
     7141 caccccctcg tcgaaccggt ctcgatgccg agcgcccaga aaaacggagg ccgggaaaat
     7201 caggcaaatc ccgtattcgc ttgccttttt gaacacggtc aatccgcttc cttccgcgaa
     7261 acaggcggga aagacctccc agtggaggat tcccgggagc aacagggaac aggcgagggt
     7321 gtgccccatc agaagcgggg agacgtcgat ctttcgtcgg aagaagagcg gggccggcag
     7381 caaggagacg ctctcggtga aacgagcggc gacccaccgt tgagtcggca ggttggtgtc
     7441 cgtccgggga aagaccccca ttcccttgta ggcgagggta tggacgaggt ccagggcccc
     7501 cacgaacggg taggcaatgc cgaggaaaag gaggcaattg ttgtcgaaga aacgccggga
     7561 attccaggcc cgggcaaaca tgccgaaggc aatgacgatg gaaaacagct cgaccaggct
     7621 gtgaaacaga agatagggcg aggccgccag ggagagcgcg accggcaacc ctgcccccac
     7681 tgccgggttg attgccttcg cggagatgga atgggttgtc atatcgggct gctccgaaaa
     7741 cacgaaaccg tcgtcacgga accgcgtggg tcgcgctctc ccccgggcgg acggaccccc
     7801 ggtcgcacgg atcgttcaca taacggcggg ctgcccggtg gatgccagca ccgtactcca
     7861 cacccagccc tggggatcga gcttctttcg gctggaaacg gccatgggga tcggcacatg
     7921 ggtgaactcc cctccccagt cccccaccac catgttggtg aggcccgcca tccccgcatg
     7981 gaccgcgttc tggcccagga gcaggcagta ggccgaatcg tgtgcgttgg ccgcaacgct
     8041 ccgaatggtg tagctgggat cgatgtactt gagattcact tctttgcccc gttccttgaa
     8101 atgggcgttg atgcgatctc gcaggaaggc gccgatgtct cccagccgga cgtttccgga
     8161 agcgtcccgg gcctgagtcc cctgcaggag gtcctggcct gcgccctctc ccaccaggat
     8221 gacggcgtgg cctttgcgct ccaaccgttc ctcgaggttt tggagaaagc cttccagggt
     8281 gaagggaatc tcgggaacga ggcagtagtt cacatcgttg taggcaaggg cggcaaaagc
     8341 ggggatgaac cccgattccc gacccatgag cttcaccaag cccaccccgt ttcgggcccc
     8401 ggtggcctcg gtgtgggcgg agaggatggc gtccccggct tcgctcaccg ccgtctcgaa
     8461 gccaaaggac cgctggacaa aggaaatgtc gttatcgatg gtcttgggga ccccgatcac
     8521 ggagattttg agcctccgct cggcgatctc ccgggagatg gcctgggctc cccgcagggt
     8581 tccgtccccg cccagggtga agaggattcc cacgttcatt cgttccaggg tgtccaccat
     8641 ttcggaaacg tcctggggtc cccgggacga ccccaaaatc gttcccccct gttcgtgaat
     8701 ccgggaaacc gattgggggg tcagttccag gggagcatgc cggaagcggg gggaaagtcc
     8761 ctcgtagccg tagagaaaac cgaagaggtt ccgaaccccg tagtggtgga agaggctcat
     8821 caccacggat cgaatcacgt cgttcagacc cggacacaag cctccgcacg tgacaatgcc
     8881 gcacttgagc ttggtgggat cgaagtagat tttctctcgg ggccccgctt tttcgaagga
     8941 aggcgggacc ttgccgccct cgaggaactt catgacggtc cggggatcgc ggtgatagag
     9001 cacccggtcg tcttcaggga caaaatgggc gcaggacatg ggagagggga tgcggcattc
     9061 tcccagcctg gctatggtga aatcaaactc cgtttccgtc atcgtattcc tcctgctgaa
     9121 cgccgggcca tgggtggcga gccgtgtggt gcgaaggacc ggctcgaatc aaagtgcccg
     9181 accatcatag cagaaaaggc cccggagcgg gaaatcagtg gctcgtcgag ggggcattcc
     9241 ccggggactc agccccgggt tggacccttg ttctggcaca acggggcaat attggggtaa
     9301 aatccaccgt cctcgatgaa cccccgggca agagagaggt gtgaggaaaa ttccatgcgt
     9361 tggtccatgc cttttccgtt gaacatccgt cagaaggtca tggtggggtt gacggtcatt
     9421 cttctggtgg tcatttccat cgggaccctt tcctaccggt acctgcgtga aatcgaacga
     9481 aaacagcact tcgtcgaggt ggcggacgac ctgagcaaca tcattctcga gatgcgccgg
     9541 tacgagaaga actacctctt gtacggttcc cgggaagacg tggaggagaa ccgccgctac
     9601 gtcctccaag gcaaggaggt gctgaagaag atgacgcccg aggcccggga gtttcaggca
     9661 ggtcctttgc tgatccgcct ggagcaggag ctgtggggct acggcgccct gatgcagcga
     9721 accgtccaat gggccgaagg ggacaaaacg aagccggatc cccgctggga agagcaattg
     9781 cgggagcacg gcaagagcct cgtggacctt tcccaggaga tggtgcggtt cgaacgaagg
     9841 aggattctca agatcataca gaccctcaag acccagttgc tcgcttccct ggccatgtcc
     9901 gcgctcctgg gcgttttctt gattcccgtg gtggcccaca agatcatccg gcctttgcgc
     9961 accatcgaga atacgaccca tcgcattgcc cggggggagt tcagcccgct gccggttccg
    10021 gaaaccccgg acgagaccca gcaggtggtg caggcgttca accggatggt cgtcgagctc
    10081 gagaagcacc aggaccaact ggtacaggcc aagaagctct cttccctggg gaccctcacc
    10141 tccgggatcg cccaccagtt gaacaacccg ttaaacaaca tctcgacctc ctgccagatc
    10201 ctcctggagg agctgaatca cccggattcg gaactgatgc ggaggatgct gggcaacatc
    10261 gaacaggagg ttcaccgtgc ccgggatatc gttcgagggc tcctcgagtt ttcccggatg
    10321 agagagttct gccttgcgcc cacgtccttg ccggaggtcg tcgagcgttc catcggcctc
    10381 atatccagcc agattcctcc ccgcatcgag gtggtggcgg acattccccc ggatcttagc
    10441 ctgagcctgg atgcccagcg aatgcagcag gtgtttttga acctgatcga aaatgcgatc
    10501 caggctatgg aaccacccgg gcagatccgg attaccgcgg gccgggtgcc cgaggaaggc
    10561 gcagtcctga tttccgtggc ggacaccgga aagggtatcc ccgagggcct cctgggcagg
    10621 atcttcgatc ccttcttcac caccaaggaa gtgggggtgg ggacgggttt gggactttcc
    10681 gtggtctacg ggatcattca gaagcacggg ggaaccattt ccgtggaaag caaaccgggg
    10741 gaaggaagcc ggttcattat ccggctgccc gccgaaaccg tatgctgaag gagagggtag
    10801 ggaagtgaga ggaccgcgca tcttgattgc ggatgatgag cgcattgcga gggaaaatct
    10861 cgagcacgtg ctccgaaagg aagggttcga cactacttgc gtggaaaacg gcgagtcggc
    10921 catccgggag ctcgaacggg aggagttcga tctcgtcatg accgatcttt gcatgcagcc
    10981 ggtggacgga atgcaggtcc tcgaacgggc ccgggagctt taccccgaca cggaagtcat
    11041 cgtcatcacg gggtacgcca ccgtaaactc cgccgtcgaa gccatgcaaa aaggcgccta
    11101 ccactacctt cccaagccct acaagatcga agaggttcga gccctggtgc gaaaggctct
    11161 ggagaaaagg tccctccggc aggaagtcac ggccttgagg cggcaaattc gggacctggg
    11221 ggaatcttcc ctgctcatcg gcaagagcgc cgagatggag gctctcaaga gaaccatcga
    11281 acaggtcgct cccacggact gcaacgtcct catccttggg gagaccggca ccggcaagga
    11341 actggcggcg aggatgattc accggctcag ccctcggcgg gacaaccgtt tcctggccgt
    11401 caactgcggg gctttcaacg aagagctgct cgccaacgag cttttcgggc acgatcgcga
    11461 ggccttcacg ggggctcggg gaatcaagaa aggactgctg gaagctgccg aaggggggac
    11521 ggtgctcctg gacgaaatcg gggacatgcc tccccccatg caggtcaagc tgttgcgggt
    11581 cttgcaggaa agaaagttcc tgcgggtggg cggaacggag gaaatccccg tggacatccg
    11641 ggtcctggcg gccaccaaca aggatctcaa acacgaggtc gagatgggct cgtttcgccg
    11701 ggacctcttc taccgcatca acgtcatcac cctgcatgtg ccgcgcctgg cggaccgaaa
    11761 agacgacatc cttctcttga gcttccactt cctggaaaag atctcccggg cccagggcaa
    11821 aaagatcgaa gccatgtccg accgggtgac ggaaattctt ctctcctacg agtttcccgg
    11881 caacgttcgg gagttggaaa acatcctgga acgggcggtt accctggcac ggggaactac
    11941 catcgaggtg gagcaccttc cgtcggatct ccgcaggccg ggccttcggg ttcacggacc
    12001 ccggcggggg tacctttcgc tggaggaaaa cgaaagggaa tacatcgcct ggattctccg
    12061 ggaggtgaaa gacaacaaga cccgggcagc cgaagtgctc ggcatcgacc gggtctccct
    12121 gtggcgcaag ttgaaacgct accggatgga atgaggccgg ggcaagaacc catgcggcag
    12181 ggtcccccga gccttgctat gcctccgcat atccgggttc cagagtggtg tgggccgatt
    12241 tccagcccag gatcatgctg cccacgatga tgacggcccc ggttcccagc gccaggcaca
    12301 tggtgacgaa gctcgctcga tcgagcacgc cgaggatccc ggaatccagg gaaaccaacc
    12361 ccagccggtg gaggtagatg ggaaccgcaa gggcccggct gaatgccaca atgagcatga
    12421 tggtcgccat cacggtcttg atggtgtgtt ctttgacata cgtggttcca atggccccca
    12481 gttggacccc gagaatggac cccgccagga tgatcaacgt cagccggatg tccaccatgc
    12541 cgtaggcggc ccagttgatc gtcccggcaa ggcccatgac gaaagcgatg accagttcgg
    12601 tggcggtgga aacgaggctt gaaaccccca tggcgtagat catgcccggg actcccacga
    12661 acccgcccac ggcgatggtg gcggcaagaa cgcccgtggc aaaccccacg ggaaccgtaa
    12721 accacaacga gattcgcacg ttggcggtct tgaaatgaat catgggggga aggtgaatct
    12781 tttggagctt caaagccagg gccgagacct gctctttccc cccggatttc cacgtcttcc
    12841 aggcatcccg ggaaacatag cctccgacca ccaggagaat gctcacgaaa gacaggctca
    12901 cgtaaaggtc cgagcctgcc ggtccccaac ggtccaggat gaagtgctga atccggattc
    12961 ccgcctggac gccgactccc gccgatgcgg ccagcaccag ccccagcttg atatccacct
    13021 ggccgtattt gaaacgcttg caggccccca ccaaagcctt gggaaactta tggcacatgt
    13081 tgctggccac cgcgatggtc cccggaacgc ccaggctcat cattcccggg gtcaggacga
    13141 aagcccctcc cgatccgatg aacccgctca cgagccctcc gataagacct accaggaaaa
    13201 gaaacccgac ggacagcggg tccaaatgga taaactggat tgcttcattg accggaacca
    13261 ttctttctct cctcctgcgt gtgaagtatc agttgccgga tacccatgga tacgcccgtc
    13321 ctaggccttg tcgatccggt gaaaaggatt gacgtacgca taagcccggg gccttttctg
    13381 agcactcttc ttcttccggg acgctttggg ctcggtctcc ctcaagaccg gtttctttct
    13441 cgcctcgatg cccaacaggg accaaagatt ggcggcaaaa gccccgtggg cgaaggaaaa
    13501 gaggaagacc gtccccacgg gaaacgctgc gtaccaaccg ccccgggtaa aagttcccat
    13561 gacggcatcc gcattccaga agacggctcc gtacatagcc gccgtgagta acccgtaggc
    13621 gagggtgcgg gccacgggtc tcttccttgg tactccctgc tccttggtca tgtcggtacc
    13681 tcctttggaa tccattgaac ctgaaatcac gctgaacatg ggaatcccca cttccctagc
    13741 cacttcttcc cggttcactc ccagatccgt caccacgaat tcgatgcgcc ggatctcgtg
    13801 ttggagctca tccaccgccc agcccgggtc tcccgccttg accgcgtgtt cgcaccggat
    13861 tcccttttgg gcggccctcc gcttgagcgt tcccgccgcc ttggccgccc accggtccgg
    13921 atatccgcgc cttcccgact catccacgct gagggccacc aggtcgtagt ccaggcgttc
    13981 cgccaggtgg acggcatagc tcacggtttc ctcggaaaag gcgtctcccg ttccaaccac
    14041 cagaatcttt cgacgctccg tgctgccctt gcccgccgat tggtttgtgg gatcgtcttt
    14101 cgtcatggtt tcacctccta tccgtgtgcc gcatgcccaa tggttggcat tgaccttgcc
    14161 gtactatcaa cgagcatgcc atgggagaat ttgttggcta actagaggaa atcacgctac
    14221 ctattgccga aagaccccca gtttccgcag tctcgttgtt tgttgcattc tgcaacggaa
    14281 aaaaagagcc gggagggata gggggccgag atttttggtc ttttgaaggt attgcgccaa
    14341 agagcacgga ctgttgcagg gtgcaacgcc gggaaaggat ggaggcatga cgtttttccg
    14401 aaaactgcgg ggacaggagg aggagaccga ggacgcgagc cgggatctcc aggagttgcg
    14461 agaggctttg gagagggctc ggcttccgga agccgtgggt ggtgtggccg ccaaggagct
    14521 cgagcgcatt cgcaggacgg attcttccac tcccgagtac accatcgggg tgaactacct
    14581 ggaatttctc ctggcccttc cctggaatcg ctttaccgaa gacaacctgg atctcgggcg
    14641 gggggagagg atcctggaag cgcagcatta cggcttgagg cacgtgaagg agcggattct
    14701 cgagtatctt gccgttcggg ctctttgcag tctccgggac tttcacattc tggtggtgga
    14761 cgacgagccc attgcccgga cgaacctgga atatgttctc cggaaagagg gctaccgggt
    14821 gagcacggcg gccaatgggg ttgaggccct ggaaatggtc ggaaagaggg aattcgagct
    14881 gatcctgacc gatctcaaga tggaaaagat ggacggcatg cagctcttgg aatccgtcaa
    14941 gcacgtggcg cccggcacgg agatcgtgat gatcacgggg tacgccaccg tgagctcggc
    15001 ggtggatgcg ctgaagaaag gggccgtgca ctatctgccc aagcccatcg atttgaacga
    15061 gctgcgctcc accgtgcggc agatccggga gaagaagcgg tttttccagg tgacccgcag
    15121 ccccgtcctt tgtttttccg gtcctccggg aaccgggaag acctccatcg gtcgttccat
    15181 cgcggaagcc cttcaacgga agttcatccg gttttccctt gccggagtcc gggacgaggc
    15241 ggagttgcgc gggcatcgga ggacctacgt cggggccatg gctggccggg tcctcaacga
    15301 aatgaaacgg gtgggggtca agaacccggt tttcatgctc gatgaaatcg acaagatggg
    15361 ccgggacttc cggggagacc cctcgtcggt cctgctcgaa atcctggacc ccgagcagaa
    15421 cggccgtttc atggatcact acctggatgt tcccttcgat ttgtcggcgg tcctgttcat
    15481 cgccacggcc aacctggtgg aggggctgcc tgaggccctg gtggaccggc tcgaaatcat
    15541 cccgttccat gggtacacgg agagagagaa gaatgaaatc gcccacaggt acctgatccc
    15601 tcgtcagatg agggagcacg ggctcacgga ccggggcgtg gaatttgccg cgggagccgt
    15661 cggcaaggtc atccgggact acaccctcga ggcggggttg agaaacctcg aacgggaaat
    15721 ggccaccatc tgccggaagc tcgcccgagt aattctctta gaccgggaaa ccccggctcc
    15781 cttggaagtg gatgaaaccc tggtggaaaa actcctgggt cctccccgat accgccatga
    15841 aatcgccgaa gaggggtttc ggctgggagt gacgacggga ttggtgtgga gcgagctggg
    15901 aggcgagatc atttcggtgg aagcgggcct catgaagggc agcgggcagc tcatcctcac
    15961 ggggtccctg ggaagcgtgc tcagggaatc tgctcaaacg gccctgagct atattcgcag
    16021 ccatgcccgg gagctttgcg tcgatcccga attcttcagg ggccacgacg tccatattca
    16081 cattcccgca ggcgccgttc ccaaggacgg accctcggcc ggcataacca tcgttttggc
    16141 attggcgtcc ttgcttacgg gaaggcccgc ccgccgggat gcggctctgt ccggggaaat
    16201 cacccttcga ggccgcattc tgcccgtggc gggaatccgg gagaaggttc tcgccgctca
    16261 acgggccgga atcaaggtgg tggtttttcc ccggcgcaac cgcttggatg tggacaatct
    16321 cgaagaggac gtgaagaacg gaatttccgt gattcttgtg gacgagatcc cggagttggt
    16381 ggatgtggtg cttcagaaga ccccgaaacc gtgaaccccg gagtcagggg ggaccggaaa
    16441 ggattttgga accggattcg ggtcccggtt ggatggacga aaaacctcgg taaacgagcg
    16501 aatccgtcgc caaagtcgta aaacgccgta cccagcgttc aggttgtccg tgccgtggtc
    16561 acgtcgttca atannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaaaacg
    16681 ccgtacccag cgttcaggtt gtccgtgccg tggtcacgtc gttcaatacc cgggccagtt
    16741 cctggaggcg gtacggtttc gagatcacgc cggaaaatcc gtatttccca aaatctccca
    16801 tgatgggatc gttggaatag ccgctggata cgacggcccg aacatcgggg tccatctcgc
    16861 gaagccgcac tacggtctcc ttgcctccca ttcccccggg aatcgtcagg tccaggatca
    16921 cggcatcgaa gagctccctc gaggataggg attcgcggta tcgttccagg gcttgttccc
    16981 cgttgcgggc caggacaacc cggtagccca gctgccccag catttcgccg accacctctc
    17041 ctacgatctc ttcatcgtcc atcaggagga ttctgccccg gcccgtgggc aattggcgct
    17101 gtgggtgcct ttcctcggca atttcccggt ccgaggcctc gaggtagatg tgaaaggtgg
    17161 tgccggaacc cagttccgat tccacggtga tgagtccacc gtgcttggcg acgatggaat
    17221 gaaccacggc gagtcccagg ccgtttcccc ggtgcttggt ggtgaaatag ggatcgaaga
    17281 tcttgggaag atattccctg gagattccgg tcccctcatc ccgaatggag attttcacgt
    17341 agtttcccgg ttccagcggc agtccgtggg ttgagctcac cgccaggttt tccgcccgca
    17401 ccttgatgag ccctccctcg ggcatggcct gatcggcgtt gagcaccagg ttgtgaatga
    17461 cctgcccgat ttggccttca tcgacctcgg cgggccaaag gtccggggct atggagaact
    17521 cgcaccggac attggagccg gttagggcaa acagggtgga ggaccggatc aggtcggata
    17581 tggaatggat cttcttgatg ggagtgccgc ccttggagaa ggtgagcagc tgctgggtga
    17641 ggtccttggc cctcagggag gctttcaggg tttcctcgag cctgcgggaa atcttcgcat
    17701 ccggcttggc atccatcatg gccagcgaga tattgcccat gatcgccgtc aagatgttgt
    17761 tgaagtcatg ggcgatgccc cccgccagca gcccgacgga ttcgagcttc tgggccttga
    17821 agaattcctc ttccattctt gtcttttccg tgatgtcgcg aaacaccagg accattccga
    17881 ggatctcgcc tccctggtcc aggatggggg ctccgctgag ggcaaccatg cctttcctgc
    17941 cgttgcgggc caccaggacg gtatgatccg gctggtcgat gatctccccc ttcctgatga
    18001 cctcctcgac gggatgctcc caacgctcac cggtgctttc gtcgaggatt tcgaagactt
    18061 ccccgaggga ccttcccacc ccttcttcct gggaccagcc ggtcatggct tcggccacct
    18121 tgttcatgag ggcgaccttg ccgttcacgt ccgtagtgat caccccgtcg gcgatgctgc
    18181 gcagggtcac ggcaaggagt tccttttccg cggccagggc ttcctgggag cgctcgacct
    18241 tgttgaggtt ttcctggaga ttccggtagg tggccgagag cttggcggac atttcccgga
    18301 aagcggcggc gagctgtgag atttcatctt ccccccgggg attgggtagg ggaacatccc
    18361 attcgccgcg actgaccgag gccgcggcgg ttgccagttg gtggatgggt ttcaccagcc
    18421 tgtccgtgaa ccacaggaag agcaggcttc ccaggatcag tcccggcagc agaatgattc
    18481 ccgcactttc caggaacttg gttcgtgcct cgaggacttc ctcggaagtc accgaaagga
    18541 tcacggtcca gctccagtcg gggtaggtat cgaagcaata gaagcgcgca tcctcgcccc
    18601 cgagacgttc cattgaacct ctgccttcgc ggatcatccg gtggaggggc gagggtcgta
    18661 tccccttgtt cccctcgagg gggtccagga gcagggttgc ggtacggcgg tcggcaatgg
    18721 cgagctttcc cgtcgagccg aaccggtagt tgcgaaactc ggcgatcagg tcttcctggg
    18781 cctttcgcac gtagctttcc acgtggccga ccccggcctc tttgagcacc tggtgggccg
    18841 agcggatggt ggcgatcagg ttgtgcactt ccatggacat gagcttccgg ttgagatcga
    18901 tcaccacctg gttgatggtg aggatgccca cgagtgcgac ggcacaagtg acggccgtca
    18961 agatgcccag ggagacgaaa ttgatcttgt acttgagctt catacggggc ttctcccgct
    19021 ccttgactct cgttgtccct tcccgtgggc aagggttatt tgcggtccag cgcttcctgg
    19081 cctatgacga cgacgttcct gagggtcgag atggggagca ccatgcccgc tgccttggcc
    19141 atcttgagat tgaccgtcac tttgctctgc ttgttgaccg tgacgggaat atctgcaggt
    19201 ggggtcccgt ccaggatctt cagcgcggtt tgagccgcat agcttccctg ctcttcgggc
    19261 aatttcgccg caacgaacat gacgaaggga gccatgaagg gattggggga gccggtggga
    19321 atccttgtgt ttcgagccag gaattcctcg gcggcaacag ggtcccaggg ctcgatcccc
    19381 gtgtaattga aaaagaagag catgtccgtt tcgctttggg cccggaggaa ttccctctga
    19441 aaatcctgga aggtcttcac caggtacacc ttcatccagc cgttgaaaaa ggtcttgttg
    19501 tagtagtcga cgatctttct ctcggtttcc acgtccccgc tgaggtagcc gatgcgtccc
    19561 cctttggcgt gccctttgaa caggttcatc atttcgtcca tggggtccac ctcgaccatt
    19621 cccgtcacgt tcttgcaggg atacccgtac atggaggcgt cccagttcac cccgctgaag
    19681 accacgggca gatcggtgtc cttcaagtag ggcaccacca ggtatttttg cgcgttgtcg
    19741 tccgtggcga tcaccacatc gggtttgaac tcttccactg cggcccgggc tctccggccc
    19801 gcttcctttc cgaactcgac cgaatcatgg cgcttggtgt ccatgtgcag agtgtggagt
    19861 tccaccccgc tgtccaccag cacctgccgg atccctcggc cgatgccttc actccatggg
    19921 tattccccgt ggtaggaatc cacccacagg attctttttc cccggtaacg gccgggctcg
    19981 gagacttccg ggacggcatt gccttcggca tgggcctcct tgcccaaaac gtgggcaatc
    20041 ttgagcaccg acacgggcag gacaatgccc gcggcttggg ccatcttgag gttgacggtc
    20101 agcttggccc tgcggttggc ggtcagggga aaatccgaag gtttcgcccc gcccaggatc
    20161 ttgagggcgg tttgggcggc gtactcaccc tgctcttccg gatacttgcc catgacgaaa
    20221 accacgaatc gatccatgta ctggacggat gcggcggtgg gaactcgggt gttcctggcg
    20281 agaaattttt ccgcagcaat ggggtcccag tcccgaatcc cggcataatt gaccaggaag
    20341 agcatgtcca cttcctgctg tgcccggagg aactggtcct tgaacccctg gaagtcttcg
    20401 gcgaagtagg ttttcatctt cccgtcaaga aacttctcgt tccagatccg gctttgttcc
    20461 ctttcggtct gggtgttccc gctcaggtac cccacgcgat cgcccgaggc gtattgtttc
    20521 atgtgacgta acatttcctc gaccagttcc acctcgatca ttcccgtcac atttccgcac
    20581 ggatatccat acatggacgc atccatgttc acgccggcaa acaccacggg aagctccgaa
    20641 tctcgaagat acggcaccac gaggtaattc tgggcgttgt cgtcggtggc gatgaccacg
    20701 tccggtttgt attcctccac gacggccctg gctttgacgc ccgcttcttc cccgaaaccg
    20761 acggaatcgt tgtttttggt gtccattcga aagatcctga gatccactcc cgtcttgtcc
    20821 aggacgtcga ggatgccttt ctggattccg tcgctccatt catagccggg atggtaggaa
    20881 tcgacccaca ataccttctt gccggaatac ctcccgggga ggtcttcctc ccgggcttcc
    20941 ttgaccggca acgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnttgccgg
    21061 aatacctccc ggggaggtct tcctcccggg cttccttgac cggcaacggg gtgtcctttc
    21121 ctccccagga tccctgcgtc catcccagga aacccggaag gagggacgcg atcgccacat
    21181 acaggatggt cctcaagctt ctcttggtca tggtcctctc tctcgggacg attgcacgcc
    21241 aacctccccc cgtttcgggg aacgtctccc cggaaaggcc gactcccgtc ccttgtgttg
    21301 gtcctccggc ccggaggacc aaccgtctcg ccgcaaaatc ccgggggaac cggtcgtgta
    21361 tcggaagaat ttccctaacg aaggacggaa aatccgcact ttcggaaacg gctccgggtc
    21421 caccccaccc atggcgccga cgcctagctc atgacgggca ggtatactcg aaacacggtg
    21481 ccccgaccgg gcttgctctc caccgtgacc gctccttcat gggttcgcac gatcccggcc
    21541 actacggcca gccccaggcc ccggccggtg aatttggtgg tatagaaggg atcaaagacc
    21601 cggcccatgg ccgacgagtc catgccgcag ccggtatcgg aaacctcgat acaggcgtaa
    21661 ttctcgtctc tcggcgtcca gtccgggggg aaaaaccggg atgccgccac ctctgcggac
    21721 ggcacttcca ggatccgcac cgtcacctcc cccgactgcc gatccaggga ttccgcggca
    21781 ttcgtcaaca ggtgggtgaa aacctgcccc atttgccagg catcccccag gacgacgggg
    21841 tcccgtgggg gaaattccgt gaggattcgt gcgctcccgg gcagggaggc gccccgggag
    21901 gacaggactt cccggcaggc tgcgcacaag gcgatgggtc ccatcttcct cgggtcccgg
    21961 cccagataga ccaacaggag cctgccgatt tcagctgccc tgcgggaagc ctgcatggcg
    22021 gcgactacgt tggatcgaga ccggagatcc tccgggaggt tgttgagggc caggtccagg
    22081 tttcccatga ccacctggag caagttgttg aagtgatggg ccacggctcc ggccatccgg
    22141 cccaggctct cggctttctc cacctgttgc agtcgtcgtt cccagcgccc tctatcttcc
    22201 tcggcctgcc tgcgctcgct catatcccgg aggatggtgg aagtcctggg gtctcccgtg
    22261 cccccctcaa aaatgaggct ggtgagttcg ccggggaagc gggtgccgtc cttgcggatg
    22321 caggtcaact ctccctggaa gattcccgag cgggcgcgtt cctcgatggc cccggctacc
    22381 cgggggtccg cagggtcaag cagtccggac cggcccaccc ggatcagttc ttcttcggtg
    22441 tacccaaaca tttgacaggc ggctggattg gcgttgagaa tggtcccgtc gggcctcgtg
    22501 aggaagatgg cctcggcgct gtggtgaaag atcgagcggt agcgttcctc gctttcacgc
    22561 agggtttctt cggcccgctt gcggtccgtg atccgctgaa aacagacgca gatgcctctg
    22621 tcccggggaa aaaccctcac gtcgtaccag ccctcaaaag gctcgatgtc gaaatgggtt
    22681 tcaaaggcca ggggaatctt ctcccggacg gccaagcggt acttttcatc gaagatcgat
    22741 ccccgccctt cgggaaaggc gtcaaaaaga tgccttccca ggacttcgga actctggcga
    22801 tggagcattc gttgggccgc cgcgttgaag taggtgagca ccaggtcctc gtccagggag
    22861 aagaaggcgt cgctgacgct gtccagaaca ctctcgagct cttttaccgt ccggcgaagg
    22921 gtttccgcct cgattctgtg ccgggaatct tccatcttcc tctcttccgc ggcgggatat
    22981 aaatcctgat ttcggttgct tgcgcgggcc ttagggaact gccgatgcct ggttgtccct
    23041 ctccgagtcc gaacgccatg tccggagttc cggaataggg atcaaaggtt cctttccgtg
    23101 ccttgatttt ccctgtcagc cggcaggatt ccgccgggaa aaggtcccgt gagcgtcggg
    23161 agaatccgga gtgcttcccg gtggtccttg gaaaaaagac gactcctgcc cgcaacatgg
    23221 gtatgcaaga tttgcgctta ctgggaaggg agcgtcccct ttgtgcggat ttgccgacac
    23281 cttttcagcg aagccctccc cccggaaggg aacccgcaat ctcccttttc agtccttcca
    23341 cggtgtagcc gtatcccatc cccatgggcc tcccatcctt ccccatgggc cacaggcctt
    23401 ggatcccgtt gacgattcgc agtcttcctt ccggatcccg tttcacgaac accaagaccc
    23461 cggatcccgt ttccggaaaa gtgagttcat cctcgcgcca tacccgcttg cccttgtgga
    23521 caaggcgacg ggtcgtgaaa tgcatcggct ttcccttggg ccaccgaccc ttgaggacct
    23581 ccttgggaag gacggtgttt tccagggttt cttccgctct gcccccgccc ttgggaacct
    23641 cgcccgtctt cttcacctcc cccacacgcc ccacgacgat aaaatccgat tgtgcgatca
    23701 gttcggggat tggcagggtt cgtatcctcc ccgaagcaac ccctgccgac agaaccagga
    23761 cgacgacggc aagacagact cctctcccca cccgcctacg actcttcctc atgccttgtg
    23821 ctccttctca taaaaggttc gggaaccgga agacggtgcc gggccccttg gttccccgag
    23881 ggcagtcggc cgtgaggacc gtcccgccga caaaagccga catccaggat gtgcccgggg
    23941 tgctgttccc tcccaacccg ggcggcaacc cctgcatcta cgtaggaaaa accggagacc
    24001 ggcctcttct tccgcctcgg gaaaccggcc gaacggcggg ctcatgccgg aaaaggtccc
    24061 tccgagcacc tcgtggaaaa ttctcctgtc cgagaacccc cggacgcggg gtccccgtta
    24121 ccctcggttg cggcagttcc cgggaaagcg gttggaaggt gcttcctcca tggcccgcgg
    24181 gaagccggtg acgagctcga ggtcgggcgg gccggggacg aagatctcgc ccgcgggcgc
    24241 tgcgtcttcc cgcaccggtt tcagcctcct gaagccctac gaccaaaatg acccgtttgc
    24301 ggggcattga cctctgtgtg ctgtaaccgt cgtgcggagg ctaccgcttc agggcctcca
    24361 gcagtcggga cttgagctct ccgtcgctca gtcgcttgtc cgcgggggtc ttgaacggag
    24421 aaaaaggact caggccaagg cagaagcgaa cctcttgaag gaggtcttcc tcgttgacca
    24481 ctgtgctcag cctttgggcg gtttccttcg atttctcgct cgcccttgca taccatttcc
    24541 cagaacgttc gatgtagttg gcgacggcgg tccggacggc ttccgctctc aagtgttctc
    24601 gatgcttttc ctcgatgcgc ttcctggtga gaaggggagt cactggcccc ctcacgtcga
    24661 actcaaactg tgcctctctg tgatcctgat tcttaaggaa gtccgtcgcg gacatgacgg
    24721 ggctatgtgc aatgtcgttg gggtccagca actgaacctt gacattggca aggggcacac
    24781 caaaggcacg cagatggtcc accacgagct gcaactgggt cggagtgggc cggtaatgac
    24841 cgctgaagtt gttcaggtac gtcaatacgc cgttcaccac cgttagacag ccggtgcaaa
    24901 tcacatcgct tccgcccgta taagaggaat gatagaaagc acctcccggc tggtctttca
    24961 aggagtgccg gaatgcgtag agactgcggt tcagggtcat gcagaaaccg gatacgcctt
    25021 tgggcttttc tttcttgctt ttgtcgtagt tcaactcgca tcccaggtga caactgtttc
    25081 ctccggcctt ggtccattct ttcttggtgg tgtcgaaggc ggtgagcgcg ttggtgacgg
    25141 tgtctcggta atagagcttg ccgccttcga tatgaatgcg gtaggcaagg gcttcggcgt
    25201 cagtcaaata tacgagggcc ttggtgcctt tctgtaaggc gtcgacctct ttgccgtgcg
    25261 gggtcaatcc gatcatctgc ctttggagaa ggatgtcgat ctcatcgccg gggacctcgt
    25321 aaatccggcc cagctcgtct gcggccgcaa agtgcacgcc gcggtaaatt gcgaaaaact
    25381 ggcagtcgcg atccgatctc tgctcatcgc gcagaactcg gcgcatgaag gtccccgtca
    25441 acgagtagat atccacgaga agggacccta agatgctgtt atcgagtttg tctttggagg
    25501 aatgggtcaa ggaagcggtg ttatagcgct caatggccgc gcccaagaga tccggatcgg
    25561 taaggtgcgg gccgatcatc ttgctttcaa ggtgggagat ttcgaaactc ttggaggcga
    25621 acttttgcag atcgaagtcg tccaggcttc tggacccttg ggtcgacgag gatgacgatt
    25681 tgctcttgcc gaaccccgga tccgattcga gcaatcgcgg ctttttccgg gactcccgga
    25741 agcaggacag gcccgtgagg gcggtcgtga ccaggtttct ccattcgtca attctcggca
    25801 tcttcttgaa gtcggtgctt tgcccgatgt gcttaccgct catcaattcc cgttgctcag
    25861 gtgtgatatt gggtttgtta gccatgacaa tccctcctac aaatttgcac agatccggca
    25921 acatgatttg ggacgactca ggcttctatc cgtaccatac agcttcccgc ctcttctcca
    25981 ctcttgcccc ggggaacaga cgggtcgccc cggtcgactc aacccgcctt ggtagttcca
    26041 ccgcagacgg gagtccggaa acttcaaggt ctcccgggtc ctttcgggtt tgatgaatcc
    26101 tgctcggtgg ttccatccct tctgtcgtgt gccctcggtg gtttttacag agtcgcagcc
    26161 ggagctcccg acgggggatt gtgttccccc cggttcatga gcccgagaac aatcatggtc
    26221 tgggtcctca cacggaccgt agtttctgaa gtcgcccctt tggagaagag gatgggggaa
    26281 ggcatcggag cggcctcttc gcaacggggt ccgaggatcg aaaccatccg ggaccgggct
    26341 tcctgttgtt ttccggattt ctccgggacc gcttccctcc cggtgcaggc aactaccggc
    26401 aaaaacagga tctttcagga tgggagtata gaaaagaact ggggggatgt cgatgagaaa
    26461 ggggcgtggg atggggctga ggggacgggg ccgaacgcgt gtgccggctt tgaccgaaag
    26521 gtgagtagcc gcacagccgc cgcgcaaaaa cccagcgacc ccccatgggt tcccaagggg
    26581 cggacccgcg gcgcaccggg tgcaacagac gggcagaggt cgaattgctt cggacctggg
    26641 aaacgtgttt cggcgacccc ggcaggacct ccgccggagt ttgccatata cccgctttac
    26701 ggttatgctt tgtctcaagc gatgaattct cgaaatcacc ccaatatggg ccccaagagg
    26761 cagcaagagc gggccgaacg ggcaagggac ggatcgtcca aatcggaaag acggccgaag
    26821 gggccgggcc cttgcctgag gggggaacat atcggaagat ggaattggaa gaagtccaat
    26881 gccccgggac cccttgcgtg cccataaccg gcgcatgggg gacgacccct gccgggagaa
    26941 ggggtcgggg ttcgggaccc gggactgacg gttgacgggg ggcaggagcc atgcacagat
    27001 ttcagaacat actctacgcc tgcaccggga ggggggacga gactcaaggg ctccagcagg
    27061 cgctcagcct ggctcgccac catcgggcgg ccttgagggt ctttctgatc cactcgccgc
    27121 ttccaaaggg cctggaagcc tatagggaaa gcttccacgc atttatctcg agacagatcg
    27181 atgcagccgt tcaaggggcc cgggcgaccc tgaacctggg tgaggccgag gtgccggtta
    27241 cggtggacct ggaaagcagc gacactccgc cggcgattgg cgtcgtacga caggtcctgg
    27301 ttcgtgggca cgatctggtc atcaaggagg cggaacccca agagggcggc aggggtttca
    27361 actcgacgga catgaccctt ctgcgcaagt gtccatgtcc tgtgtggctg gcgcgaccga
    27421 tccgccgcgc tcaaaacgag atccgcgtcg ccgtggcggt gagtccggaa agtcgagacg
    27481 agaacgagtc gtacctgtcg atcaaactgc tccggattgg gcgctctctg gcggatacgt
    27541 tcagcggaga actggacgtc atcagttgct cggaccactc gttcgaggaa tttctgcgcc
    27601 ggaacacccg ggtggagatt cccgaagaaa gcatccttag cgccgccctg aaactgcaaa
    27661 gccggcatga cgccgaactc cgtcgcctga tcgaggattc cggcatcgac ggcccctatc
    27721 gcatccgtta cctgaagggg caccctgaaa cggtcatccc caactttgtc gagactcatg
    27781 ctgtcgatat cctcgtgatg gggaccgtgg caaggtccgg gatcgtcggc tttctcatcg
    27841 gcaataccgc cgaaaacgtc atgggccggc tcggctgtac tctgctggct ctcaagcccg
    27901 atgcctatgt ctcgcccctc gctgagctgc cgctcaagcc gtgagccggg atatgggcgg
    27961 gatgtcgccg ggtgtttcga agcaccccac ccccctggag ggcgagggtc ccggggaaga
    28021 tggccgcaac gacgtctccg tataatgccg cgtgaccttc tgcggccagc cggcgcaccg
    28081 tgtcgaggtt gagatcgatg acggtgattc gggcgccgcg ctcccgaagg agctggtgga
    28141 cgattctccc caccggtccg tatccgacca ggatgcagcg gttgggatgg atcggggcgt
    28201 tcccccttcc gatcgccggg agcggtccgg cggtcctcgg cgtcgccata cggcgcgccc
    28261 accgatagat gtacgggtcc aaggcgatgg aaaggatgga tgcggccccg agggcgtgcc
    28321 ggccgctctc gctgagcact cccaggatga gggccaccgt gcccaggatg aaactgaatt
    28381 cccccacctg ggaaaaacgg ggccgacggg gattgccgtc gacacgggct ggcccggagc
    28441 gcgaacgatc atgccgaata cccggcccgg tttgggtacc gtttggatga gttcgggcgg
    28501 ggtcccgccc tcattcttgg gggtgacacg tgcccggcat tcgactccgc ccggagtccg
    28561 aaaagttcgg atcagccggg ctcctatctc ctgcccccga ggatcgagcc caacaccccg
    28621 cggatgatct ggcggccgat ggaacttccg atggaacgca atgcgctctt ggcgaacgcc
    28681 tccgccatgc tctcccgggg acgtcccggg cttcgacggg aaggcggttc ttcataacga
    28741 ggttccggct gcctataggt ccggggcggt tcttgacagc gaggttccgg atgtccgtaa
    28801 gccggcggcg gttgggcttc ccgacggtcc cgggccattc tccggtccgt ccggtttcgg
    28861 agtatctcga aggccgattc ccggtccacc atggtttcat aattgccgaa atgaatggag
    28921 tcccgaattc ggtctcgtcg ttcctcgggg gcgatcacac ccacgcggct ctcgggcggg
    28981 cacacaaagg cgcgttggac cacggcgggg gttccgtctt cttccaggaa agaaacgagg
    29041 gcctctccca caccgagttc cgtgatcgcg tcttcgacgg cgagtttcgg attcggacgg
    29101 aaagtctgcg ccatgatcct gaccgctttt ttctcctggg gcgtgaaggc ccggagagcg
    29161 tgctggacgc ggttgcccaa ctgggccagg accttctcgg gaatatcctt cgggttctgg
    29221 gtgacgaaat agacgcccac ccccttggaa cggatcagcc ggaccacctg ttcgatcttg
    29281 tccagcaggg ccggtggagc gtcctcgaaa agaaggtgcg cctcatcgaa gaagaagacc
    29341 gtttgcggct tctccgggtc cccgacttcg ggcaggcgtt cgaaaagctc cgccaggagc
    29401 cacagcagaa gggtggcgta gaccttggga cgcgcgatca gttgatccgc cgccaggata
    29461 ttgatgatgc ctctgccgtc cgagtgggtc tggatgagat ccaggatgtc cagcatcggt
    29521 tcgccgaaga actggtctcc cccctgggcc tcgagctcga tcaggccgcg ttgaatggcg
    29581 cccaccgacg ccttggaaat gtttccgtat tgggtggtga actcccgggc gttgtccccc
    29641 acgtactgga ccatggagcg aatgtccttg aggtccagaa gcaggagacc gccgtcgtcc
    29701 gcgatcttga agaccagggc caataccccc gtctgcacct cgttgaggtt caacaagcgt
    29761 cccatgagca gcggtcccaa gtcggaaacc gtggcccgga ccgggtgccc gctctttccg
    29821 aggacatccc aaaaggccac ggggaattcc cgaaatcgat ggtccgaaag tccgagcagt
    29881 cggacgcgtt ccaggatctt ggggttgtcg tctccttttt ggctgatccc ggccaggtcc
    29941 cccttcacgt cggccaggaa aacgggaatg ccgttgtcgc tcagcctctc ggcaatccgc
    30001 tgcaaggtca ccgtcttccc cgttcccgtg gccccggcaa tgaggccgtg ccggttggcc
    30061 atggaaggga tcaggcaaaa ctcgaccttt ttggaactct tggcaatgag catgggttgg
    30121 gccatgattg aagcctttct ttgcgaggga tgacgaacaa ccgtcggcgg cacgggattg
    30181 ttgtcatagg gggaggggat tgtcaaggaa gggcgggcgc gggggcctgc ccctacggaa
    30241 acctgcgaac ccctggcgga aaacacaacg cagccatgct gggtatgagc ctacgaatgc
    30301 ccgaaatttc caaccgggaa ggaattgtcg tctgccgatc caacaagatg ttggcgccgc
    30361 ctgcggcgtc gccaatgcgg cgttgcccct atcgggtccc tgt
//
LOCUS       DAOOVC010000038        29935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1048_length_29935_cov_7.386747, whole genome shotgun sequence.
ACCESSION   DAOOVC010000038 DAOOVC010000000
VERSION     DAOOVC010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 29935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29935
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_29935_cov_7.386747"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="PLM79_07130"
     CDS             complement(<1..442)
                     /locus_tag="PLM79_07130"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOV86119.1"
                     /translation="MAELRVLFCSPEVSPYAKTGGLADVAASLPGPLKGLGCDVRIFM
                     PFYRSCRDAASVTSLAENIPIRVGTQTYHVHFRQAVTPSGVPVYFLEKDEFFDRTYLY
                     GTPTRGDYEDNPERFITFCRAAHALCLQLNWFPDVFHLHDWQSAL"
     gene            633..1715
                     /gene="ispG"
                     /locus_tag="PLM79_07135"
                     /gene_synonym="gcpE"
     CDS             633..1715
                     /gene="ispG"
                     /locus_tag="PLM79_07135"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOV86120.1"
                     /translation="MHETRRRLTRQIHIGEVPIGGSAPVAVQSMTNTDTRDYEKTVRQ
                     VRLLEELGCEIVRVAVPDETAVQQLSRIKKAIGIPLIADIHFDHRLALGALKAGVDGL
                     RLNPGNIGGPSRVRKVVEAAKERQVPIRIGVNSGSLEKDLLARHGRPTPEAMVESALR
                     HVALLEQLDFDLIKISLKSSDVPDTIASYRLLSQKTDYPLHLGVTEAGTLVGGTVKSA
                     LGIGLLLYEGIGDTLRVSITGPPEEEIPIAYSILRTLHLRERGIELVSCPTCGRTEIE
                     LIPLVKEAERILGKVKSPLKVAIMGCVVNGPGEAREADVGIAGGKGKGILFKKGERVE
                     KVDESDLLPRLLKEIEIMTGESLTAG"
     gene            1864..4014
                     /locus_tag="PLM79_07140"
     CDS             1864..4014
                     /locus_tag="PLM79_07140"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOV86121.1"
                     /translation="MRFFEIIDTVLGYYPEANTALLEKAYVFAARFHSGRPRFREEPY
                     LFHPLAVAGILTRMKLDEESIAAGLIHHVLEANPSVREEVEEEFGPNVLRIVQGVTKL
                     SGLNFSSRKEKQTEYLRNMILALSQDVRVLLVRLADRLHDMRTLPCEGSDGQKLLARE
                     TMDIYAPLAGRLGIEWIKQELENLAFRCLDPEAYEEITRGLANTEEDRRRYTEEMILV
                     LTANLKEYGVQGTVSGRPKHLYSIYQKMMRQNLDLQRVYDLIAFRIIVDSIKACYEAL
                     GMIHDLWEPVPGRFKDYISKPKSNMYQSLHTTVIGPYGERMEVQIRTEEMNRTANEGI
                     AAHWIYKEGKPLGKIDHQETQRYSWLRELLEWNQPWKDPKAVFEEGRLDFYPDEVYVF
                     TPQGDVKVLPRGSTPVDFAYEVHSEVGHRCVGARVNGKLVPLRQELQNGDTIEILTSP
                     NHRPSKDWLKFVKTSKALTRIRHWIKAEERERSLSMGREVCEREFRKKGLNFGNYLNS
                     PELLEAARSLSFKSVDDLLANIGYRKISPTQVIGRLPSLAPAEPVEKEPEALASERRK
                     TAAPSGGIKVRGADDVLVRMARCCNPVPGERIVGYITRGRGITVHHALCRNLSRGDSD
                     RRIEVEWDSGKGKVFPVDVKVVCAKEKGMLAGLSGVLGQLDANILDVHLDSSASDATM
                     CLFKIEVKDNKHLQRVLLALKAERGVYQVQRSTL"
     gene            complement(4082..4369)
                     /locus_tag="PLM79_07145"
     CDS             complement(4082..4369)
                     /locus_tag="PLM79_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOV86122.1"
                     /translation="MAIRVLVEREIEPGNELKLHQTLTKLRAEATLAKGYISGETLRA
                     LDNPNKFLVISTWNSIEDWKNWQNNAKRKKFQEELRELMRCAEKTIVYTYL"
     gene            5130..5402
                     /locus_tag="PLM79_07150"
     CDS             5130..5402
                     /locus_tag="PLM79_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV86123.1"
                     /translation="MTKAELVSKIAGDGKIAKVQAEKAVDGFISAVSAALAKGEKVTL
                     VGFGTFSVGKRSQREGRNPRTGEKITIPSSKVVKFKPGKSLSDKVK"
     gene            6498..8435
                     /locus_tag="PLM79_07155"
     CDS             6498..8435
                     /locus_tag="PLM79_07155"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOV86124.1"
                     /translation="MAKNLTYKILESHLVEGRIAPGEEIGIKIDHVLVQDATGTMVML
                     EFEALGLRRIRAELGVQYVDHNILQSDFKNADDHRYLRTCSARYGLHFSPAGNGISHQ
                     VHMERFGIPGKTILGSDSHTPGAAGLSMLGIGAGGLDVAMALAGYPYYLPCPLVLGVR
                     LTGRLPDWVSAKDVILEMLRRYGVKQCVGKVVEYYGPGVQSLSATDRETIGNMGTELG
                     ATSTLFPSDDNTRTYMEAQGRGSSWRELYPDEGAHYDEHAEIDLSRVEPLIACPSSPG
                     NVVPVREVAGIPVHQVIVGSSVNSSFRDLMVVAKILEGKHCRPSVSFNVNPGSRQVLE
                     NVAEHGGLVSLLQAGARIHESGCLGCIGMGQAPGTGEVSLRTMPRNFPGRSGTKDDQV
                     YLCSPETAAAAGLKGVITDPRDLAKEMPYPRIQDPRRYVIDEKSIVVPSEDLAQTEVV
                     RGPNIKPLPSMDPLPENLELEVALKVGDNISTDTIMPAGSKVLPLRSNIGAISEFVFS
                     QVDPDFAARCARKGAVLVVGGENYGQGSSREHAALAPRYLGVRAKIAKSFARIHKANL
                     CNFGILPLTFHDPEDYEAFRQDSRVSFSNIRERVARGDREIPILVDGKTVLTLLDVSE
                     RQRKQILAGGALNLVKDGLPT"
     gene            8535..9728
                     /locus_tag="PLM79_07160"
     CDS             8535..9728
                     /locus_tag="PLM79_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOV86125.1"
                     /translation="MEQTVRVKNIGLRGVTVADTKISYIDGEQGILIYRGFRIEELAQ
                     MSTFLETAYLLLEGTLPDKEQLRTFNDRVVRERRLPDFLLQTLKGWPKNAHPMDVLQA
                     SVPLLGMADPDLSLNTREANVRMAVRLIARLPAVVAAWHRIRNGLDPLPPDDGLSHAA
                     NFLWQLLGKKPDEEDARDLDVCLILHADHTFNASTFACREVVSTRAHLYAGVAAGIGA
                     LSGALHGGANARVMDMLFDVLSKTQSPEEVRDWVRQKVRSGERIMGMGHAVYKTVDPR
                     SKILKAMSRKMAEKLGRSRLYDTLILIEDEALKELEKKGKTNIKTNVDYFSGSVYSMM
                     GIPVDLMTPVFAISRVSGWCSHIIEEKFAEAQDKPALYRPEAEYVGDYCGSIGCEYRP
                     VEARS"
     gene            9754..11133
                     /locus_tag="PLM79_07165"
     CDS             9754..11133
                     /locus_tag="PLM79_07165"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOV86126.1"
                     /translation="MKTRPEKDALGTVLVPVDAYYGAQTQRAVENFPVSGLRFSRSFI
                     EALGLVKKCAAMVNETLGLLDPALAPAIVRAAREVADGKLDDQFVLDVFQTGSGTSTN
                     MNANEVIAGRANEILTGRRGGKSPVHPNDHVNLGQSSNDVIPSVLHIAALGAIRRNLL
                     PALESLRDSLRQKSEEFRDVLKIGRTHLQDAVPVPLGREFGGYARQIDLAIERIKRTG
                     ESLAELALGGTAVGTGLNTHPEFAARVIEILSRETGIEFREAADHFEAQAAQDAAVEA
                     GGALRGLAVALIKIANDLRWLASGPRCGLGEIALPALQPGSSIMPGKVNPVIPEVVIQ
                     VAAHVMGNDLAISYGGQGGYFELNTMLPLIAHNLLQSIELLSSVTDLFRTKCIRGIVA
                     RREECSRKIEQSLALATYLVPRLGYDQSCAVSQEAFRTGKTIREVVLEGRILSPEEVE
                     ELFRDIRAR"
     gene            11311..12249
                     /locus_tag="PLM79_07170"
     CDS             11311..12249
                     /locus_tag="PLM79_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOV86127.1"
                     /translation="MNNPATLNVTRLSKIKLFLALSRTQHGLLDMATPAVAALLALGA
                     IPSPRVILLGILTAFAGYTAVYAVNDVVDYRVDRDKIRKCGISQGPGDLDAVCVRHPI
                     AQGLLSLPEGIAWALGWGVVAVLGAYSLNPVCAWIFLAGCAAETVYCLLLKVSYLRTL
                     VSGWVKTAGGLAAVYAVVPDPSPTFLGTLFLWLFFWEIGGQNVPNDWADRKEDADLEA
                     KTIPVRFGPEDSARIILGTLAAAVCLSLLLYWATPTRMSLLYLPGTLALGGFCLLLPA
                     YKLHRSLDPVLASALFNTSSYYPLGMLLLVWLSILL"
     gene            complement(12262..16968)
                     /locus_tag="PLM79_07175"
     CDS             complement(12262..16968)
                     /locus_tag="PLM79_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86128.1"
                     /translation="MPDEHLTRRSIDFQHQETFFRLINPEDYPAFGIDPEDVPMGTFA
                     SEDHPSFLPSRFGGNAYGLGLIEQSVLTTADTDFLESLDFQDVQEISRNRRRLNAIYQ
                     KLGLLMRFSHTGKRYFLIPINLVAHSLQEIKTKADEIEELIIRHIWETRTERLDIGLL
                     TRGQDLIVHELTARLSSHRIFLFESLDKLRSWRLPLDILLLPKDLFEYLLEQPLPRRS
                     KKPLSRYRLYHYAMYLAGKLYDIIEPTGKLLVLANAPDPEEDLTCRVSFKSREDLKFF
                     LLFSHIFKTKRIYRSAPEQGEMEIHLADLHYFLNRFALLEPHIRRLLDHQKPEDLSLE
                     EIDRLPRLNLRLPQTFVKNPEKQWKWIFEPYFTTSQLVKKSPTQHREYWEQRLEIDRE
                     LPESLYVFVGQPRQPEVTPASIEEAVRSSGMQGCSLSLVAEYRNSFDYVLEVLKILAQ
                     IRDHDFPKLSELERTRLSNAFHNRNENFNAVIKLLNQVPRLERIREILNPENIEGPRT
                     PILENLSKLFLHGFTAAQLKEMLLIMVGYSTMSRIVFGKLPEKTLRSITDRAREDNYR
                     EIVDLLRVCRLMSMAEIAATLGESFTGEQAKELYRLYDDAIYVATDPRLDWERLEDLR
                     ISALGGVQNKAIREMLKLFNLFEFLDTWQDFMRRGPSQKEVICDYQPERLRHLQEALE
                     LAGIANDFKQKFMSDHIFGHSHFFRRFLDTEFHGTGHLFPMLGTRAGFILLWIAVNAS
                     DRHIINFNPLLAGVPENRREQRIGKIREALLRLSVENLQPSLFADVKQDLNEGRPAFI
                     FDTGLRITSQEEQRVIEVSFVDVDENIRQLEALLTRFESQRLRGVSLRNLQELERLFS
                     ELESFHHYLQQRGCQLQCDRFNLSEGFEKKDLEIGGIRERLKSNLQGQIFVPEEVYDS
                     ISVLAQHCPEVLRFILPEFHAFGSLVETWPTRRQQSLGRYVMRCLEKFQALIIKDRNT
                     FQDRNTFYQLAKQEFGPLAEEGIGATHAQMEILEYITERIQERMNLCQALNLSLVFQD
                     IGKVDAYRASLPELADSWTHAEVGAEILEKSDILRRLPFGSQVDQLVIRLVRDHGVVG
                     HVIQGEEPIIALKPLIETGDDRFLDALALHSIVAAAAVQEGLLTSDLLDLFLNYRALA
                     LRLLRAKTDWHSWLREHIREKGETVLEEFRLTSRRVGDSAARPIPTCGWEQTESCDEV
                     LWHGRQSVAMERLFRLVGVTWVDYQDLQMHLLEMPVNFIYHKKKLKSVGVEAFQRQLA
                     DAAQMYRVVSEMTPELRCYLLFSLDHLGGALRIYHFHHLPRYLEIREAVKLLVITLQV
                     FHHHYGIEARNGLISFGELSQKIEKRQNLIQEMLRELPFPERCLMEPGPQLLTHPHGR
                     IRFQKGLHQVALAVGYEDAIRLRDMADSLEAFSSHDDLVRHYREMVQGLERDMPYETS
                     DAREELRRVFERQRERINERILGTYRERMGNIHTFSRFQQIQDEIQASQWVLNLTERQ
                     RFLLHEMVEFHRSRLREHYLDSIYREIRAFEEKSDLTAYWDRLKHELYSLRPSVGKEF
                     ESLVAQFMDRRLEELS"
     gene            17167..17640
                     /locus_tag="PLM79_07180"
     CDS             17167..17640
                     /locus_tag="PLM79_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOV86129.1"
                     /translation="MRGKRLSTWGRLATLGGMGNIPVAPGTAATFLAGIPAAYALKSL
                     PIPLGLFFLLILLALSCRACSRAEEELGEKDPGRVVLDEIVGYLVTILFLPQGFGPLL
                     WGFAAFRLFDIWKPWPIRSMEARVSGGLGIVLDDVLAGLYAFALTTMLCFAFNRP"
     gene            complement(17671..18270)
                     /locus_tag="PLM79_07185"
     CDS             complement(17671..18270)
                     /locus_tag="PLM79_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOV86130.1"
                     /translation="MRLDQPKARRWLRNPWMWIACFFILQFLGSLGVIRSLKVDLWDV
                     YPALAFLLAGFGVSAALAGLGGRMRMTEGTVRWLPAVLYALFIFSLSHRSFSSVSASV
                     NSDLFHPLEYWSFSLLLSWALEPLFEVRSMRSFSLAVLGIGLVYGIGDEIHQAFIPGR
                     TPSLLDLALDMAGVAAGWGTFMVLRHLFRSTPQKRPILS"
     gene            18428..19231
                     /locus_tag="PLM79_07190"
     CDS             18428..19231
                     /locus_tag="PLM79_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOV86131.1"
                     /translation="MIGLIPVFVFLGFAGPGTDRCSALTAEEKNTISVYERVAPSVVN
                     ISTEVCDLDFFQCAVPGAGTGSGIVLKEDGLILTSYHVVAQARDIQVTLSDDRHLKAR
                     IVGTNPEEDLALLKVDVGQRPLTPIVNGDSRQLRVGEKVLAVGNPFGLGQTLTTGTVS
                     MVGRDVRSGRLILRDLIQTDASINPGSSGGVLVNSRGEMVGMNTAILSPTGMSAGIGF
                     AVPTHQIDKELSAMRYPWPPWARWILPALFGYWVFRRITRLGRREQHDL"
     gene            19228..20385
                     /locus_tag="PLM79_07195"
     CDS             19228..20385
                     /locus_tag="PLM79_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOV86132.1"
                     /translation="MKRNPFDPRGMLPRAFNGQETRNRPWRRAVGCLLWVILVVGVLP
                     DSGHSMTEDERNNIEVYQRLAPGVVNITSTVLERDFFFNAVPREGAGSGSIIDLAGHI
                     LTNHHVIEDARKLEVTLANGKRYPAKLIGSDPDTDVAVLKIDAKRSDLTVIPMGDSDN
                     LKVGQKVLAIGNPFGLGQTLTTGVISSLGRTLKATSGILVEDIIQTDASINPGNSGGP
                     LIDSSGKMIGINTAIFSPTGANVGIGFAIPIDTAKTVIGELIDRGYYAYPWMGASLLT
                     LTRDLAESLKLPVESGAMVLEVVPRGPMEKAGIRGGNARAQVGNTIITVGGDVIVRVD
                     GEAVTSAHGLIRKIRKHRPGDRVRMEVVHWEGGRETVTVTLGEKPKNPLKR"
     gene            complement(20636..22072)
                     /locus_tag="PLM79_07200"
     CDS             complement(20636..22072)
                     /locus_tag="PLM79_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV86133.1"
                     /translation="MRFFFDIILVKMVTLGWAVNLTPLVLAFFLRRRWDTPLDRGLIL
                     RDGEALFGPHKTSRGVLGGVVAGLVVGLPLGFPWWLGLSCGILGMGGDLLSSFLKRRA
                     GLEAGRVVPGLDQVFEGGLPLLVLAPYFSLNLLQAAVYLGIFGLGAYGGSVLLQRTLL
                     SKPFEGYPRPVRPDVRFRELRSCRIQNDPFRTLFNFEDAVYYHMLMKTTFRVLGLYEK
                     GKENALRVRSRRVVFTFRDLPPSFDGYTVLFLTDLHLDGVDGLTEKVQSMVRDLPADL
                     CILGGDFRMENSGSSAEALSRLRRLLPEIRARDGILGVLGNHDCIELVEPLEKSGMSF
                     LLNDAVRIPRGGDEVWIVGVDDPHYYRCHDPGAAFASVPSGAFSVFVAHSNEIYREAS
                     RYRPQLYLCGHTHAGQIRVPYLGAVFTHSRAPRRLSYGTWSHDGMMGYTSCGVGVSGI
                     PVRFASQGEIVLIRLRRGDPPSASVLDP"
     gene            complement(22157..23458)
                     /locus_tag="PLM79_07205"
     CDS             complement(22157..23458)
                     /locus_tag="PLM79_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV86134.1"
                     /translation="MTTYSSENCIPLDLLLTKSELQDDFDNLFQEGMMEGEFAETDLA
                     LDTPEAEEEREEAGHETGYGGEEVEEEDLPAGGAALSEDFEDDPVTCYLKEVAGYPLL
                     TQEREIELAQIIRRGEEELVRMVEEHADEDSALVDLQGKIEKLICHEKTFPGVRDKIL
                     KVILRTLERGAEISSNTPLFSDLLRRTRSILASIDLAKQEMVKANLRLVLSIAKRYRG
                     RGMSFDDLIQEGNLGLLKAVGRFDHTKGNRFSTYATWWVRQSIIRGIYDKTRTIRLPV
                     HFIEMKNLFYKVFYELLKELGREPMPHEIAERAKLPLEKVQTVLTLASQPISLETPIG
                     DDEQRLGDFVEDDRAASPMEECSDRELAEMTRVLLSSLQPREEKILRLRFGIDGQPAE
                     TLEKIGRSFKVSKERIRQIEKKALRKLRNPNRQSTLKTFID"
     gene            complement(23745..24731)
                     /locus_tag="PLM79_07210"
     CDS             complement(23745..24731)
                     /locus_tag="PLM79_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOV86135.1"
                     /translation="MPANLPPHYYEAEKRFREAKTSEGKIEALEEMLTIMPKHKGTDK
                     LRADLRRRIGKLKDQAQQKKGGARRAATFSIEREGAAQVLVVGAPNVGKSSLVACLSN
                     ACPEVAEFPHTTWKPTPGMAPFENIQFQLVDTPAITLERIDPGMTDLMRRADILVILV
                     DLHADPLQQLEDTLKILEGLRIYPLGRPVPGDLPKMPFVKKILVAVNKVDEEGDEEDY
                     RAFLELSEWKLPCVGISTRTSRNLGAFLRMVFDQAQIIRVYTKPGGKDPDMTAPFILA
                     KGATIEDLAEKIHKDFRNKLKFAKVWGKGVFDGQMVQRDYVLQDGDIAEIHL"
     gene            complement(25148..27805)
                     /gene="alaS"
                     /locus_tag="PLM79_07215"
     CDS             complement(25148..27805)
                     /gene="alaS"
                     /locus_tag="PLM79_07215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOV86136.1"
                     /translation="MKATEIRKAFLEFFRDRGHTVVKSSSLIPHDDPTLLFTNAGMVQ
                     FKRSFLGEEKRPYSRAASSQKCMRAGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFK
                     RDAVECAWAFLTDRMGLPKDKLYATVHEGDATMHLGPDEEAKGFWARYLPQDRILEFP
                     TKDNFWAMGDTGPCGPCSEIIIDQGEKMGCGGPDCKPGCDCDRYLELWNLVFMQFNRK
                     EDGSLEPLPKPSIDTGMGLERIAAVKQGVPSNYDTDLFSPMMSRIEELTGYRYGSYGE
                     KDVSVKVIADHSRAAAFLINDGVLPGNEGRGYVLRRVIRRALRHGRFLGLDRPFLHDM
                     AVAVMTSMEDAYPELLESKSFITRVILHEEERFNETLDFGLKLLQSEVRRLQDEGEKS
                     IPGSLIFKLYDTYGFPIDIISDTVRDRDFRVDEAGFQELMEKQRKQSRLHWKGSGERE
                     IAEVYRQLSALGLKTVFLGYETLVAWSKVEAVILNETAVQEAETGEEIELVTSETPFY
                     GETGGQVGDRGEILGPSGRIEVLDTMRLPGDLFVHVGKVLEGSVRVGDEVTLKVDEAL
                     RQDTALHHTATHILHAVLRKVLGDHVKQAGSLVAPDRLRFDFSHFAALSPEEISEIER
                     RVNLEIRRNQDLEVRVMGLEEAMKTGAMALFEEKYGEEVRLVEIPGLSRELCGGTHTH
                     RTGDVGLFVILQETGIAAGVRRIEALAGRRALEYVQKQQTVLRQAAGLLKTGVVELPD
                     RIERLLNAQKQMEKELESLKASFAARRSADLLAEAGQIGSATVLVTRVEADNPKVLRE
                     MNDRFKERLKSGVVVLGSVHEGKVFLLVGVTPDLVKRVHAGELIREIVREVGGSGGGR
                     PDMAQAGGNRPEKLDDALDLARRLIEERL"
     gene            complement(27832..28866)
                     /gene="recA"
                     /locus_tag="PLM79_07220"
     CDS             complement(27832..28866)
                     /gene="recA"
                     /locus_tag="PLM79_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOV86137.1"
                     /translation="MGANDDRQKAIDTAISQIERSCGKGAIMRLGEGALQVEVAVIPT
                     GCLSLDLALGIGGIARGRIVEIFGPESSGKTTLALHIIAEAQKLGGLAAFIDAEHALD
                     VHYARKLGVQVEDLLISQPDYGEQALEIAEILVRSNAVDVIVVDSVAALVPKAEIEGE
                     MGDPHVGLQARLMSQALRKLVSSISKSRTSVIFINQIRMKIGVMYGSPETTTGGNALK
                     FYATMRMDIRRVAPIKEGQEMVGNRTRVKVVKNKIAPPFKEVEFDVIYGRGISKEGDV
                     LDLAVDTGIVDKSGTWYSYGGERLGQGRENAKSFLREHPDLFLEIERKTRQAHNLKIP
                     PGGPDAVAAD"
     gene            complement(29046..29612)
                     /gene="thpR"
                     /locus_tag="PLM79_07225"
     CDS             complement(29046..29612)
                     /gene="thpR"
                     /locus_tag="PLM79_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOV86138.1"
                     /translation="MIRTFVALDLPMTVRQSLGGLIEAFRKSGAAVAWVKGERIHLTL
                     KFLGNVAPERIDGIKDALGKAAASVSPFRLQPGGCGAFPSLKQMRVVWVGMKGDLEGL
                     STLRSRVEAALAPLGFEPEGRRFRPHLTLGRVKGPRGNRELTEILLANLAFETEAFDV
                     SDLVLYKSDLKPDGAHYTPLYRAGFARG"
     gene            complement(29587..>29935)
                     /locus_tag="PLM79_07230"
     CDS             complement(29587..>29935)
                     /locus_tag="PLM79_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOV86139.1"
                     /translation="TYSNRAKVDLLGVDPELIGSLGAVSEPVARAMAEGARRKAGTDV
                     AVSVTGIAGPTGGTPEKPVGTVFIACSTALGCEVEKHRFKGSRGHIRECAAQAALVLL
                     WRVLSDDSNLCGS"
BASE COUNT         6163 a         8945 c         8774 g         6053 t
ORIGIN      
        1 ccagcgcgct ttgccagtcg tgaagatgaa acacgtcggg aaaccagttc aactgaaggc
       61 agagggcgtg ggccgcgcgg caaaaggtaa tgaaccgctc ggggttgtcc tcgtagtctc
      121 cccgggtcgg agtcccgtat aagtaggtcc gatcgaaaaa ttcgtctttt tccaaaaagt
      181 agacgggaac tcccgaaggg gtcaccgcct gccggaaatg aacatggtag gtctgggttc
      241 ccacccggat gggaatgttt tcggcaaggg acgtcacgga agcggcatcg cggcagcttc
      301 ggtagaaggg catgaagatg cgcacgtcgc acccgagccc cttcaagggc ccgggaagcg
      361 aggcggcgac atcggcaaga cccccggtct tcgcataagg ggacacttcg ggggaacaaa
      421 aaaggacacg cagctcagcc atgattccgc tcttgataag gagtcaatcg ccaacaggtc
      481 acccggtatg accattacta tgaatcccgg tcccagaaaa gcactttgtg ttcctatatc
      541 ttggcaaggc cctgcgaatt ctttagaatt agcgcgaatc cgaccccgtc gctcccgact
      601 cgatcacatg aaagccatct tgggaggttg atttgcacga aaccagaaga agactcaccc
      661 gacagattca cataggagaa gtccccatcg gcggaagcgc tcccgttgcc gtgcagtcca
      721 tgacgaatac cgacacccgt gattacgaaa aaacggtcag gcaggtccgt cttctggagg
      781 aactggggtg tgagattgtg cgggtggcgg tgcccgatga gacggcagtc caacagcttt
      841 cccgcatcaa gaaggccatc ggcatccctt tgattgcgga cattcacttt gatcaccgcc
      901 tggccctggg agcactgaag gcgggagtgg atgggctgag gctcaatccg ggcaacatcg
      961 gggggccttc ccgggttcga aaggtcgtgg aggccgccaa ggagcggcag gtgcccattc
     1021 gcatcggggt gaacagcggg tccctggaaa aggacctcct ggcccgccac ggccgtccga
     1081 ctcccgaagc catggtggaa agcgcactgc gccacgtggc cctcctggag caactggatt
     1141 ttgatctcat caagatctcc ctgaaatcct cggatgtgcc ggacaccatc gcatcctacc
     1201 gcctcttgtc ccaaaagacg gattaccctc tgcacctggg ggtgaccgag gcgggaaccc
     1261 tggtgggtgg caccgtgaaa tcggccctgg gcataggact tctcctgtac gaggggatcg
     1321 gggacactct tcgcgtctcg atcaccggcc cacccgagga ggaaatcccc atcgcctaca
     1381 gcattcttcg gacgctccac ctgagagagc ggggaatcga actggtcagt tgccctacct
     1441 gcggtcgaac ggaaatcgag ctgatccccc tggtcaaaga agcggaaagg atcctgggga
     1501 aagtgaagag ccctctcaag gtcgccatca tgggttgtgt ggtcaatggt cccggggagg
     1561 cccgggaagc ggatgtggga atcgcggggg gaaaggggaa ggggattctg ttcaaaaagg
     1621 gggagcgggt ggagaaggtc gacgaatccg atctgttgcc gcgcctgttg aaagaaatcg
     1681 agatcatgac cggggaatcg cttacggccg ggtaaaagac aatcgccggt tcccgccccc
     1741 caaggcccgc ctctccagtg gagggacgtg tcggaatgcc gaggcgggcg tcccgggcgc
     1801 gggatccgcc gcgcgggaaa ggccgatcct tccccgttta ctacgccagg gtgagtaaag
     1861 gcagtgaggt ttttcgaaat catcgatacg gtcctgggct actatcccga agcaaatacc
     1921 gccttgctcg agaaggccta tgtatttgcc gccaggttcc actcggggag accccggttt
     1981 cgagaagaac cctacctctt tcaccctctg gccgtcgccg ggatcctgac ccggatgaag
     2041 ctggacgagg aaagcatcgc cgcgggcctc attcaccacg ttctggaggc aaacccgtcc
     2101 gtccgggaag aggtcgagga ggagttcgga cccaacgtcc tgcgcatcgt ccagggcgtg
     2161 accaagctca gcgggctcaa tttcagcagc cgcaaggaaa agcagaccga gtacttgcgg
     2221 aacatgatcc ttgccctgtc ccaggatgtt cgcgtgctgc tggtccgttt ggccgaccgg
     2281 cttcacgaca tgaggaccct gccctgtgaa ggctcggacg gtcaaaagct cctggcccgg
     2341 gaaaccatgg atatctatgc tcccctggcc ggccgcctgg ggatcgaatg gatcaagcag
     2401 gaactggaaa acctcgcctt tcgatgtctg gaccccgagg cctacgagga aatcacccgg
     2461 ggactggcca atacggaaga ggatcggcgc cggtacaccg aggaaatgat tttggtactg
     2521 acggccaatc tcaaagagta cggggtccag gggaccgtca gcggtcgtcc caaacacctc
     2581 tacagcatct accaaaagat gatgcgtcaa aacctggacc tccagcgggt atacgacctg
     2641 atcgcctttc gcatcattgt ggactcgatc aaggcctgtt acgaagccct ggggatgatc
     2701 cacgacctgt gggagcccgt cccggggcgt ttcaaagact acatttccaa gcccaaatcc
     2761 aacatgtatc aatccctcca taccacggtg atcgggccct acggagaaag aatggaggtt
     2821 cagattcgca ccgaagaaat gaaccgcacc gccaacgaag gaattgccgc ccactggatc
     2881 tataaagagg gaaagcccct ggggaagatc gatcatcagg aaacccaacg ttactcctgg
     2941 ctccgcgagc tcctggaatg gaaccagccg tggaaggacc cgaaagcggt tttcgaggaa
     3001 gggcgcctgg acttctatcc cgacgaagtc tatgtcttca ctccccaggg agacgtcaaa
     3061 gtgctccccc ggggctccac ccccgtggac ttcgcctatg aagttcactc ggaggtagga
     3121 catcgctgtg tgggagcgcg ggtgaacggc aagctcgtcc ctttgcgcca ggaactgcag
     3181 aatggggata ccatcgagat cctcacctca cccaaccacc ggcccagcaa ggattggttg
     3241 aagttcgtca agacgtccaa ggctttgact cgtattcggc attggatcaa agccgaagaa
     3301 cgggaacgtt ccctgagcat gggtagagaa gtctgcgaac gggaattccg caaaaaggga
     3361 ctcaacttcg gcaactacct caactccccc gaactgctgg aggccgccag gagcctttcc
     3421 ttcaaaagtg tggacgacct tctcgccaac atcgggtacc ggaagatctc ccccactcag
     3481 gtcatcggca ggctcccttc ccttgccccg gccgaaccgg tggaaaagga gccggaggcc
     3541 cttgcttcgg aacggcgaaa gaccgccgcc cccagcggcg gcatcaaggt gcgaggggcc
     3601 gacgacgtcc tggtgcgcat ggcccgatgc tgcaaccccg tacccgggga gcgcattgtc
     3661 ggctacatca cccggggtcg aggcatcacc gtccatcatg ccctatgtcg caacctctcc
     3721 cgtggagact ccgaccgtag aatcgaggtc gaatgggact cgggaaaagg gaaggtcttc
     3781 cccgtggacg tcaaggtggt ttgcgccaag gaaaagggca tgctggcagg cttgagcggg
     3841 gtgctggggc agctggacgc caacatcctg gatgtgcatc tcgattcctc ggccagtgat
     3901 gccaccatgt gcctgtttaa gatcgaggtc aaagacaaca agcacctcca gagggtcctc
     3961 ctggcactga aggccgagcg aggcgtgtat caggtacaaa gaagtactct gtgaaccccc
     4021 atccacgcgg ggagaaatgg atgagggtcc caaagggagg acaatcaccg agatcgggag
     4081 cctacaggta agtgtagacg atcgtcttct cggcacagcg catcaactcc cgcaattctt
     4141 cctggaattt cttgcgcttc gcgttgttct gccagttttt ccagtcttct atgctgttcc
     4201 atgtgctgat aacgaggaac ttgttggggt tgtccaaagc ccgaagggtc tccccggaga
     4261 tgtagccctt ggccagggtt gcctcggccc gcaacttggt gagggtttga tgaagtttca
     4321 actcgttacc cggctcgatt tccctttcta ccaatactcg tatcgccatg ggacgcctcc
     4381 ttggttgtgg gtgatgggaa gagttgaaga aatccttacc caaatcccaa tagggcgact
     4441 caaccattcg gatgtgaaaa ccatgagcgt ggtcggggaa gcttaggatc cgctgctgga
     4501 cgtcgctgca cgcgcatgca tatgccgtac gaaaaaccgc cccatcgaga cgttgcgccg
     4561 ttcttaggtt cacttatagc actcatcccg caagtagtca ataacattgg ctttatttga
     4621 tattaacaaa caagagaggg aataggggca aaattccttg cagcacaagg ctttcagcgc
     4681 tgaaagttgt tgatttttcc aaaatggtgg ccttacaata ttcgcaattt tgagggcaac
     4741 cccccactgg gagccggaag accgacttgt tcggtattca tagcattcaa aacttcatat
     4801 cgtgcttctt ccgtgcgaca gccatgcatg tttccgaata ccacgggaga ttttccttgt
     4861 attatccacg ctttaccgtg ttccgtttcc agagccggtt tggacgaatt cgggaggggg
     4921 tgaaatgcgc gtcggactgc aggttgagaa tccaggatgg ctgctttcgc cccgcccgtg
     4981 aggggccacg aggaaagggg accgtcttcc ggagagaaga tgtccgaccc cgttttccgt
     5041 tagcgagttg aggggccgtt ttagcctgcc ggaaatggga ggagcgaggt tgttcttacg
     5101 tcaactcatg gaagagagga ggaaggagta tgacaaaggc ggaattggtc tcaaagatcg
     5161 cgggggacgg caagatcgcc aaggtgcaag cggaaaaggc cgtagacggg ttcattagtg
     5221 cagtgagtgc cgccctcgcc aagggggaaa aagtaacttt ggtgggcttc ggcaccttca
     5281 gcgtggggaa acgatcccaa agagaagggc gcaatcccag gacaggggag aagatcacca
     5341 ttccctcttc caaggtggtt aaatttaagc cgggcaagag cctgagtgat aaggtgaaat
     5401 aggcaccccg acaaatcccg actcctggtt tgtggacgcc cgagaggcgc tggatcgttg
     5461 tcggccggac ttcatgacgg gttattccca tctgcgggac aacccgttgt taagcggcaa
     5521 aaggtaagca cgagcaacgg caaataattg gaatattccg gaatggctcg aaaaggaggt
     5581 ccgagcacgc gatacggtgt gcgtctattg cggcaatgag tttacctcgg tgaaaatagt
     5641 gagaaagtct gcggctggct gggagcacat catcaacgat gccggcatca ttactcggga
     5701 aaatatcgcc ctatgctgct ggggctgcaa tgcgagtaaa ggccggaaac ctctatctgt
     5761 ctggctgcaa acccagcatt gcatagaccg tggcattacg ccggaaacgg tggctccggt
     5821 ggtaaaacag gccatcgaac gtagcttgta gtacagcgct taacaaggtc atgcactcgg
     5881 tcacgcaacc acgccgggca aatatggatg catttcttga agttccttgg cggcgtggtt
     5941 gcgtgccggt gatgaccagc gttatgtgga tacaatgggc ttaaccgaac tccgaaggat
     6001 ccggcaatca acgctgcgga ttgagtcgca gggaccaccc tcattgctgc gccctgccga
     6061 catcgccgga ttccccgaac cggcctcccc tcgatggaca cggcccattt agccatcccg
     6121 agcgctgtcg gacagttcct cccacccggc ttgcctcttt cctttttcag gcgattcgaa
     6181 tgattgaccc gtggtcccgg ggggattccg cccttctggg ggctgccctc tgcggactct
     6241 cccggggaaa tcccgacccg ggatcgaggc ggtgggtttt tgaccacggg ccgtgaaaaa
     6301 gccttgtgat gggtggggtg cgtacctata taggcataag ggcagggaac ggggatctca
     6361 cttggggact tcgctagatc tccgggacgg caagaaaacc gttgggaagc cgcgtgaggg
     6421 cattggaaca agagcgggct ccgagatgcg gggctccggc aagagaacag gaagaatcgc
     6481 cgggggtgga ggaaactgtg gcaaagaacc tgacctataa gattttggaa tcccacttgg
     6541 tggaagggcg catcgccccg ggggaggaaa tcggcatcaa gatcgaccat gtcctggttc
     6601 aagatgcgac gggaaccatg gtgatgctgg aatttgaggc cctgggtctg cggagaatcc
     6661 gagccgagct gggggtccaa tacgtggatc acaacattct gcagtcggat ttcaagaatg
     6721 cggacgatca tcgatatctc cgaacctgtt ccgcccggta cggacttcac ttcagccccg
     6781 cgggaaacgg gatttcccat caggtgcaca tggaacgatt cggcattccg gggaagacca
     6841 tcctgggctc cgacagtcac actccggggg ccgccgggct gtccatgctg ggaatcggcg
     6901 cgggcggtct ggacgtggcc atggccttgg ccggttatcc ttactatttg ccttgccccc
     6961 tggtgctggg ggtccggttg accggccgtt tgcccgactg ggtcagtgcc aaggacgtca
     7021 tcctcgagat gcttcgccgg tacggggtga aacaatgcgt ggggaaggtg gtcgaatact
     7081 acgggccggg agtgcaaagc ctgtcggcca cggaccggga aaccatcgga aacatgggta
     7141 cggaactggg cgcgacaagc accttgtttc cctccgacga caatactcga acctacatgg
     7201 aagcccaggg tcgcgggagc tcgtggcggg aattgtatcc cgacgaaggg gctcattacg
     7261 acgaacacgc agagatcgac ctgtcccggg tggaacccct catcgcctgc ccgtcttctc
     7321 cggggaatgt ggtacccgtc cgcgaagtgg cgggaatccc ggtacaccag gtcattgtgg
     7381 gcagcagcgt caattcgtct tttcgggacc tgatggtcgt ggccaaaatc ctggaaggga
     7441 aacactgccg cccttccgtc tccttcaacg tgaatcccgg cagtcgacag gtcctcgaaa
     7501 acgtcgccga acacggtggg cttgtctcgc tgctgcaggc gggggcccgg atccacgagt
     7561 cgggctgcct gggctgcatc ggtatgggcc aggctcccgg tacgggagag gtgagcctgc
     7621 ggaccatgcc ccgaaatttc cccgggcgct cgggcaccaa agacgaccaa gtctacctgt
     7681 gctctcctga aacggctgcc gcggcgggac tcaaaggggt tatcacggat cctcgcgacc
     7741 tggcaaagga gatgccctat cccaggattc aggacccccg gcggtatgtc atcgatgaga
     7801 agtccattgt ggtcccgtcc gaagacctgg cccaaacgga agtggtgcga gggcccaaca
     7861 tcaaacccct gccttcgatg gatccgctcc cggaaaacct ggaactcgag gttgccctca
     7921 aggtcgggga caacatttct acggatacca tcatgcctgc gggaagcaag gttctccccc
     7981 tgcgatccaa tatcggtgcc atcagcgagt tcgttttttc ccaggtcgat ccggattttg
     8041 ccgcaagatg tgcacggaaa ggcgccgtgc ttgtggtggg aggcgaaaac tacgggcagg
     8101 ggtccagtcg cgagcacgcc gccctggcgc ctcgctacct gggggttcga gccaagatcg
     8161 ccaagagctt tgcccgtatc cacaaggcca atctgtgcaa cttcggaatt ttgcccctga
     8221 cctttcatga ccccgaggac tacgaggctt tccgacagga ttcccgcgtc tctttttcca
     8281 acattcgtga acgggtggcc cggggagacc gggaaattcc gatcctggtg gacgggaaga
     8341 ccgtcctcac ccttttggac gtatcggaac gtcagagaaa acagatcctg gcgggaggag
     8401 cgttgaacct tgtgaaggat gggctcccca cctgagaaag agccgaggcc tcgggagtgc
     8461 cgaagggttc ccccggcctt gtccgagctc acgcatgaac ccgaagactt ccggggatgg
     8521 gagtggagga tatcatggag cagaccgttc gagtgaaaaa catcggcctc agaggcgtca
     8581 ccgttgcgga caccaagatc agttatatcg acggggaaca ggggatcctc atctatcgag
     8641 gattcaggat cgaggaactg gctcaaatgt ccacgttcct ggagacggcc tacctcctcc
     8701 tggaagggac cctgcccgac aaagaacagc ttcgcacgtt caacgatcgg gtcgtccggg
     8761 aaaggcggct tcccgacttc ctcctgcaga cgctcaaggg ctggcccaag aacgcccatc
     8821 ccatggacgt gctccaggct tcggtgcccc tcctggggat ggcggatccg gacctgtccc
     8881 tcaacacccg ggaagccaac gttcgcatgg ctgtgcgact catcgcccgt ctgcccgcgg
     8941 tggttgcggc ctggcaccgt atccgaaacg gcctggatcc cctgcctccc gacgacgggc
     9001 tctcccatgc cgccaacttt ttgtggcaac tgctggggaa aaagcccgac gaggaggatg
     9061 cccgggacct ggacgtgtgc ctcatccttc atgcagacca caccttcaat gcctccacct
     9121 ttgcctgccg ggaggtggtc tccacccggg cccacctgta tgcgggagtc gctgcgggaa
     9181 tcggggccct ttccggagcc ctccacgggg gggccaatgc ccgggtgatg gacatgctgt
     9241 tcgatgtcct gtccaagacc caatccccgg aggaggtccg cgactgggtt cgccaaaagg
     9301 ttcggagcgg cgagcggatc atgggcatgg gccatgccgt ctacaagacc gtggacccga
     9361 gatcgaaaat cctgaaggcc atgtcccgca aaatggcaga gaagctcggc cggtcccggc
     9421 tgtacgacac cctgatcctc atcgaggacg aagccctaaa ggagctcgag aagaaaggaa
     9481 aaaccaacat caagaccaac gtggattatt tcagcgggtc cgtgtattcc atgatgggaa
     9541 ttcccgtgga ccttatgacc ccggttttcg ccatctcccg ggtttcgggg tggtgttcgc
     9601 acatcatcga ggagaagttt gccgaggccc aggataagcc cgcactctac cgacccgaag
     9661 ccgaatatgt cggggactat tgcggctcca tcggctgcga ataccggccg gttgaagccc
     9721 gatcgtgaac gggaaggaac gggagaacgc aggatgaaga cccgtccgga gaaggacgct
     9781 ctgggtacgg tcctggtgcc cgtggacgct tactacggtg cccagactca aagggccgta
     9841 gagaactttc ccgtaagcgg cctccggttc tccagatcgt tcatcgaggc cctgggactc
     9901 gtcaagaaat gtgccgctat ggtcaacgag accctgggac tcctggatcc cgccctcgct
     9961 cccgccatcg tgcgggccgc ccgggaagtg gcggacggaa agctggacga ccagtttgtg
    10021 cttgacgtct ttcaaacggg ctcggggacc tccaccaaca tgaatgccaa cgaggtgatc
    10081 gcggggcggg ccaacgagat cctcacgggg cgacggggcg ggaaatcccc cgtgcacccc
    10141 aacgatcatg tgaacttggg gcagtccagc aacgacgtca tcccttcggt gctccacata
    10201 gcggccctgg gagccatccg gcgcaatctc ctgcccgccc tggaatccct tcgagattcc
    10261 ctccgccaaa agtccgaaga gtttagagac gtcttgaaga tcgggcgaac ccaccttcag
    10321 gatgccgtgc cggtccctct gggccgggag ttcggcgggt acgctcggca aatcgacctg
    10381 gccatcgagc ggatcaagag gaccggggaa tccctggccg agctggccct gggaggcacc
    10441 gccgtgggga ccggtctcaa cactcatccg gaatttgccg cccgagtcat cgaaatcctc
    10501 tcccgggaaa ccgggatcga gttccgggaa gcggcggacc acttcgaagc ccaggccgcc
    10561 caggatgccg ccgtcgaggc cggcggagcc ctccggggcc tggccgttgc cctcatcaag
    10621 attgcaaacg atcttcggtg gttggcttca ggacctcgat gcggtctcgg ggaaattgcc
    10681 ctcccggccc ttcagccggg atcctccatc atgcccggga aggtgaaccc ggtgattccc
    10741 gaggtggtga tccaggtggc ggcccacgtt atgggcaatg acctggcgat ttcctatgga
    10801 ggccaaggcg ggtattttga gttgaacacg atgctgcccc ttatagccca taacctcctt
    10861 cagtccatcg agcttctttc ttccgtgacg gacctctttc gcaccaagtg tatccggggc
    10921 atcgtcgccc ggcgggaaga atgctctcga aagatcgaac aaagcctggc gttggccacc
    10981 tacctcgtgc ctcgcctggg atacgatcag tcctgcgccg tttcccagga agcgtttcgt
    11041 acggggaaga ccatccggga agtggtattg gaagggagga ttctctcccc ggaagaggtc
    11101 gaggaactct tccgggacat ccgcgcccgc tgacggtgga aaaacggaac gacctcggat
    11161 cgcctcggtg ggggagtgaa ctccgggacc ctcttcccgg catgggggaa gaggccggtt
    11221 ttggaattgc cttccagatc gtaaggagga gacttccccc tttgagagaa cccccaactc
    11281 ccgacggttc cgggtgcaag gagatctctt ttgaacaacc ctgccacgtt gaatgtaacc
    11341 cggttatcca agatcaagct cttcttggcc ctctcccgga cccagcacgg gctcctggac
    11401 atggccaccc ccgccgttgc ggctctcttg gccctgggag cgatcccctc cccgagggtg
    11461 atccttctgg ggattctgac ggcctttgcg ggctacacgg cggtctatgc cgtgaacgat
    11521 gtggtggatt accgggtgga ccgggacaag attcgcaaat gcgggatttc ccaaggaccc
    11581 ggagatctgg acgcggtctg cgtgcgtcat cccatcgccc aaggcttgct gagcctcccc
    11641 gaaggaattg catgggccct gggatggggc gtggtggccg tcctgggggc ctattccctc
    11701 aacccggtct gcgcatggat tttcctggca ggatgtgcgg cggagaccgt ctattgcctc
    11761 ctgttgaagg tgagctacct tcgcacgttg gtgagcgggt gggtcaaaac ggcgggaggg
    11821 ttggcggcgg tctatgcggt agtgccggac ccttcgccga cctttttggg aacacttttt
    11881 ctgtggctgt tcttctggga gatcggcggc cagaacgtcc ccaacgactg ggcggacagg
    11941 aaggaggatg cggaccttga agcgaaaacc atccccgtgc gcttcgggcc ggaggattcc
    12001 gcccggatta tcctgggcac cctcgccgcc gccgtttgcc tgagcctcct cctgtactgg
    12061 gccactccga cccgaatgag tctcctgtat ctgccgggaa cccttgccct cggaggcttc
    12121 tgcctgctgc tgcccgccta caagctgcac cgttccctgg accctgtcct ggcttcggcc
    12181 ctcttcaaca cctccagcta ttaccccctg ggcatgttgc tgctggtgtg gctcagcatt
    12241 ctcctttgaa agactcgcgc ctcagctcaa ctcctccaac ctccggtcca tgaactgggc
    12301 gacgagggat tcaaattcct tccccaccga cggcctgaga gaatagagct cgtgtttgag
    12361 ccggtcccag taggccgtga ggtccgattt ctcctcgaag gcccggatct cccggtaaat
    12421 ggaatccagg taatgttccc gaagccgcga tcggtgaaac tccaccattt catggaggag
    12481 aaacctttgt ctttccgtga ggttcaggac ccactgagac gcctggattt cgtcctgaat
    12541 ctgctggaac cgggagaagg tatggatgtt ccccatccgt tcccggtaag tccccaggat
    12601 ccgttcgttg atcctctccc tctgcctttc gaaaaccctg cgaagctcct ctctcgcgtc
    12661 cgaggtctcg tagggcatat cccgctccaa cccctggacc atctcccggt aatgtcggac
    12721 caggtcatcg tgactcgaga aggcttccag tgaatcggcc atgtcccgga gccggattgc
    12781 gtcctcatac cccaccgcca gggcaacctg gtgcagaccc ttttggaatc gtattcgacc
    12841 gtggggatgg gtcagaagtt ggggacccgg ttccatgaga cacctttcgg ggaagggaag
    12901 ctccctcagc atttcctgga tgaggttttg cctcttctcg atcttctggc tcagctcccc
    12961 gaaactgatc aggccgtttc gtgcctcgat gccgtaatgg tgatggaaaa cctggagggt
    13021 gatcaccagg agtttcacgg cttccctgat ttccaggtac ctgggcaaat ggtggaagtg
    13081 atagatacgc agggctccac ccaggtgatc cagggaaaac agcaggtagc agcgcaactc
    13141 cggcgtcatt tccgagacca cccggtacat ctgggccgca tcggccagct gtctctggaa
    13201 agcctcgacc cccacgctct tcagcttctt cttgtggtag atgaaattga cgggcatttc
    13261 caacaggtgc atctgcaggt cctggtagtc gacccaggtg acccccacca accggaagag
    13321 ccgttccatg gcgacgctct gacgtccgtg ccagagaact tcgtcacaag actccgtctg
    13381 ctcccatccg caggttggga tcggccgggc ggccgagtcc ccgacccggc gggacgtgag
    13441 ccggaattct tccagaacgg tctctccttt ttcccggata tgctcccgga gccaggaatg
    13501 ccagtcggtc ttggcgcgga gaaggcgcaa agccagggcg cggtaattca ggaacaggtc
    13561 gaggaggtcc gaggtcagga ggccttcttg taccgccgcg gccgcaacaa tggaatggag
    13621 cgccagggcg tcgaggaacc gatcgtctcc ggtttcgatg aggggcttca gggctatgat
    13681 cggttcttcc ccttggatga catgtcccac caccccgtga tctcgaacga ggcggattac
    13741 cagttgatcc acctggcttc caaagggcaa ccgccgaagg atgtccgatt tttccaggat
    13801 ttccgcaccg acctccgcat gggtccacga atcggccaac tcggggagcg atgcccggta
    13861 ggcgtccact tttccgatgt cttgaaacac caaggagaga ttgagggcct gacagagatt
    13921 catccgttcc tggattcttt cggtgatgta ttcgagaatt tccatctggg cgtgagtggc
    13981 tccgattccc tcttcggcca gggggccgaa ttcctgcttg gcaagctggt agaaggtgtt
    14041 gcgatcctgg aaggtgttgc ggtccttgat gatcaaggct tggaatttct ccaggcaacg
    14101 catgacatac cttcccaggg actgctgccg tcgagtgggc cacgtctcca caaggcttcc
    14161 aaaggcgtgg aattcgggaa gaatgaacct gagaacctcc gggcagtgct gagccaaaac
    14221 gctgatggaa tcgtagacct cttcgggcac gaagatctgg ccttgcagat tggatttcag
    14281 tcgttcccgt attcctccga tttccagatc cttcttctcg aagccttcgg acaggttgaa
    14341 acgatcacat tggagctggc agcctcgctg ttgcaggtag tgatgaaagc tttccagttc
    14401 tgaaaacagc cgctccagtt cctgcaggtt cctgagggag actccccgga gccgctggga
    14461 ctcgaagcgg gtcaagagag cttccagttg ccggatgttt tcatccacat ccacaaaaga
    14521 aacctcgatg acccgctgtt cctcctggga ggtgatgcgc aggcccgtgt caaaaatgaa
    14581 agcaggtcgg ccttcgttga gatcctgctt cacgtccgca aaaagagacg gttgaagatt
    14641 ctccaccgag agccgcaaca gggcctcccg gatcttgccg atgcgctgtt cgcggcggtt
    14701 ttcgggaaca cctgccagca gaggattgaa gttgatgatg tggcggtccg aagcgttcac
    14761 ggcaatccag agcaggatga aacccgcccg ggtccccagc atggggaaaa ggtgtcccgt
    14821 gccgtggaat tccgtgtcca ggaagcggcg gaagaaatgg gaatgtccga agatatgatc
    14881 gctcatgaac ttctgcttga agtcattggc aatcccggcc agttccaggg cctcctgcag
    14941 gtggcggagc cgttccggct gataatcgca gatgacttct ttctgggatg ggccccgtcg
    15001 catgaaatcc tgccacgtgt ccaggaattc gaacaggttg aagagcttga gcatctcccg
    15061 aatggccttg ttctgaactc cccccagggc gctgatacga agatcctcga ggcgctccca
    15121 atccagccgg ggatccgtgg ccacatagat ggcgtcgtcg tacagcctgt acagctcctt
    15181 ggcctgctcc ccggtaaaag actctccaag ggtggcggcg atctcggcca tgctcataag
    15241 acggcagacc ctcagcaaat ccacgatttc gcgataattg tcttccctgg cccggtccgt
    15301 gatgctgcgc agggtctttt cggggagctt gccgaacacg atccggctca tggtgctgta
    15361 tcccaccatg atgaggagca tttccttgag ctgtgccgcg gtgaatccgt gcaagaaaag
    15421 cttggagaga ttctcgagga tgggagtcct ggggccttcg atattctcgg ggttcaggat
    15481 ctctcgaatc cgctccagcc ttgggacctg gttcaacagc ttgatgaccg cattgaagtt
    15541 ctcatttcga ttatggaatg cattgctgag acgagttcgc tccaattccg aaagcttggg
    15601 aaagtcgtgg tcgcggatct gggccaggat cttcagcact tccaacacgt agtcgaaaga
    15661 gttccggtac tcggccacca gcgacaaact gcacccctgc attcccgaac tcctgacggc
    15721 ttcctcgatg gaagccgggg tgacttcggg ctgccgaggc tgccccacaa acacatagag
    15781 actttccggc agttcccggt cgatctccag ccgctgttcc caatactccc ggtgctgcgt
    15841 gggcgatttt ttcaccaatt gactcgtggt gaaatagggt tcgaagatcc acttccattg
    15901 tttctccggg ttcttcacaa aggtttgagg gagccgaaga ttcaagcggg gcagccggtc
    15961 gatctcttcc aggctgaggt cctcaggctt ttggtgatcg agcaatcgcc ggatatgggg
    16021 ctcgaggagc gcgaatcgat tcaggaaata gtgcaggtcc gcaagatgga tttccatctc
    16081 cccttgttcg ggggcgctcc ggtatatcct tttcgtctta aaaatgtggg aaaagagcag
    16141 gaagaacttg aggtcttccc gggacttgaa cgacacgcgg caggtgagat cctcctcggg
    16201 gtccggggcg ttggccagca ccaggagctt tccggtgggt tcaatgatgt cgtagagctt
    16261 ccccgccagg tacatggcat aatgatagag ccggtaccga ctcaagggct tcttggatct
    16321 ccggggaagg ggctgttcca ggagatactc gaagagatcc ttgggcagca gaaggatatc
    16381 caaggggagc cgccaggagc gcagtttgtc tagggactcg aaaaggaaaa tccggtgact
    16441 ggacaaccgg gccgtgagct catgtacgat caggtcctgg cctcgggtga gcaacccgat
    16501 gtccaggcgc tccgtacggg tttcccagat gtgacggatg atcagctctt cgatctcgtc
    16561 cgccttggtc ttgatctcct ggagggaatg agccaccagg ttgatgggaa tcagaaaata
    16621 tcgcttcccc gtgtggctga aacgcatgag aagccccagc ttctggtaaa tggcgttcag
    16681 ccgccgccgg tttcgagaga tctcctgaac gtcttgaaaa tccaggcttt cgagaaaatc
    16741 cgtgtcggca gtcgtgagga cgctctgctc gatgagtccc agcccgtatg cgttcccccc
    16801 gaaccggctc gggagaaaac tgggatgatc ttcggaggcg aaggtgccca tgggcacatc
    16861 ctccggatca atcccaaagg ccgggtagtc ctcggggttg atcaagcgga agaaggtttc
    16921 ttgatgctga aagtcaatcg atcttcgagt aagatgctcg tccggcatat cgggctctat
    16981 tccgccgtgt gagaagatcc agactcagta ggtccccttt ccttgcatga gtttggcata
    17041 tcacgtttga ctcgccaagg caaaccggga aggaaaccgg gacttcctgc accttaagaa
    17101 tccgggctcc cggcaggaat gccccgggca cgacatcacg agtcgactcg accatgaaag
    17161 gaaaccatga gagggaaaag actcagtacg tgggggcggt tggcaaccct gggaggcatg
    17221 ggaaacatcc ccgtcgctcc cggaacggcg gccacgttcc tggcgggaat ccccgcagcc
    17281 tacgctctga aatccctgcc catccccctg gggctcttct ttcttttgat cctccttgct
    17341 cttagctgcc gggcctgctc gagggcggaa gaggaactgg gggaaaagga tccgggccgg
    17401 gtggttctgg acgaaatcgt ggggtacctg gtgacgatcc ttttcttgcc ccaaggcttc
    17461 ggacccctct tgtggggatt cgccgccttt cggttgtttg acatttggaa accctggccc
    17521 atccgttcca tggaagcaag ggtctcggga ggcctgggca ttgtcctgga cgatgtcctg
    17581 gcaggccttt acgcatttgc cttgacaacc atgctgtgct ttgctttcaa ccggccctag
    17641 gggttccccc gtccccgggg aacgcggccc ttacgagagg atcgggcgtt tctggggggt
    17701 gctccgaaac agatgtctca ggaccatgaa ggttccccaa cccgccgcaa cgcccgccat
    17761 atccagggcc aggtcgagga ggctgggggt gcggcccgga ataaaggcct ggtgaatttc
    17821 gtccccaatt ccgtagacca ggcctatccc gagaaccgcg agagaaaaag accgcatgga
    17881 ccgtacttca aagaggggtt ccagtgccca gctcagaagc agggaaaaac tccagtattc
    17941 cagggggtga aagagatccg agttcacgga agcggagacc gaggaaaagg atcggtggga
    18001 aaggctaaag atgaacaggg cgtataggac ggcggggagc caccgaacgg tcccttcggt
    18061 cattcgcatt cgaccaccca gcccggcaag cgccgcagac acgccgaacc ctgccaagag
    18121 gaaagccagg gccggataaa cgtcccaaag atcgaccttc aggctccgga tcacccccag
    18181 ggaccccagg aactgcagaa tgaaaaagca tgcgatccac atccacgggt tgcgcaacca
    18241 tcgtcgggcc ttgggttgat cgagtctcat gagctctccg ggtcctattt actttggctc
    18301 cgttcgagat tagaaatagg atgtttcgtt ggaggatcgg caattgccgc gatcctttgt
    18361 cccgtttgtc gacatagcgg gcggttgcct gcaaaaggtc cccgatacta tgcgattctc
    18421 gaagcggatg attggactca ttcccgtgtt cgttttcctg gggtttgccg gaccggggac
    18481 ggaccgttgc tccgccctga cggccgagga gaaaaatacc atttccgtct atgaaagggt
    18541 agccccctcg gtggtcaaca tctccacgga ggtttgcgac ctcgactttt tccagtgcgc
    18601 cgttccgggg gcgggcacgg ggtccgggat cgtcctaaag gaagacggtc tcatcctcac
    18661 cagttatcac gtggtcgccc aggcccggga catccaggtg accctcagcg atgacaggca
    18721 tctcaaggca agaatcgttg gaaccaaccc cgaggaagac ctggcccttc tgaaagtgga
    18781 cgtagggcag aggcccctca ccccaatcgt caacggcgat tcccggcaat tgcgggtcgg
    18841 agaaaaagtg ctggccgtgg gaaacccctt tggcctcggg caaaccctca cgacggggac
    18901 cgtgagcatg gtgggacggg acgtgaggag cggtcgcttg atcttgcggg acctcatcca
    18961 gacggacgca tccatcaacc cgggaagttc cggaggggtg ctggtcaact ccagagggga
    19021 gatggtgggc atgaacaccg ccatcctgag cccgacgggg atgagcgcag gcattggatt
    19081 tgccgttcct acccatcaga tcgacaagga gctttccgca atgaggtatc cgtggccccc
    19141 atgggcccgg tggatccttc cggcattgtt cggatattgg gttttccgaa gaataactcg
    19201 gctgggacga agggagcaac acgacctatg aaacgaaatc cattcgatcc ccggggaatg
    19261 cttccccggg cgttcaacgg gcaggaaacc cgcaaccgtc catggagaag agccgtgggc
    19321 tgtttgctgt gggtcatcct tgtggttgga gtccttcccg actcgggtca ttccatgacc
    19381 gaggacgaac gcaacaacat cgaggtgtac caaagactgg ccccgggggt ggtgaacatc
    19441 accagcaccg tcctcgagag ggatttcttc ttcaacgccg ttccccggga gggagccggt
    19501 tccggctcca tcatcgacct tgcgggccac attctcacca accatcacgt cattgaagat
    19561 gcccgcaaac tggaggtgac cctggccaac gggaaaaggt atcccgccaa gctcatcggg
    19621 tcggaccccg ataccgacgt ggccgttctg aaaatcgacg ccaagaggtc cgacctcacg
    19681 gtgatcccca tgggagattc cgacaatctg aaagtgggcc aaaaggtcct ggccatcgga
    19741 aatcccttcg gcctgggtca gaccctcacc accggggtca tcagctccct gggacggacc
    19801 ctcaaagcca ccagcggcat cctggtggaa gacatcatcc agacggacgc ctccatcaat
    19861 ccgggaaact ccggtggccc cctcatcgat tcctccggga agatgatcgg catcaacacc
    19921 gccatcttca gtcccacggg agccaacgtg ggaattggat ttgccatccc catcgacacg
    19981 gccaaaaccg tcataggaga actcatcgac cggggctatt acgcctaccc gtggatgggc
    20041 gccagcctgt tgaccctgac ccgtgacctg gccgagtctt tgaaactgcc ggtcgagtcg
    20101 ggagccatgg tcctggaagt ggttccccgc gggcccatgg aaaaggccgg gatccgggga
    20161 ggaaacgccc gagcccaggt tggaaatacg atcatcaccg tgggcgggga cgtgatcgtc
    20221 cgcgtcgacg gggaggccgt caccagtgcc cacggcctca ttcgaaagat tcgcaaacac
    20281 cgaccgggag acagggtgcg catggaggta gtgcactggg aaggaggacg ggaaacggtc
    20341 accgtgaccc tgggggaaaa gcccaagaat cctttgaaac ggtaggagag gggatacggc
    20401 agtcctccac cctgctgcga ttgctcatcc cccggcccgt tgtaacggaa tccgggccct
    20461 gccgcgagga gcgtggaatc ggttgtgcgt gagcgttcgg ccgtccgcca tcgccgttcg
    20521 gcaacgacag gaagagcaaa ggcctgcaat tgcggcagag cgattcagga atcggcggta
    20581 ccggcatgac cggtggttct tgccggcagt taatggccga attgccgcgg ttgggctacg
    20641 gatcgaggac ggaggccgaa ggaggatctc cacgccggag gcggatgagg acgatttcgc
    20701 cctgggaggc gaaacgaacg gggatgcccg agacccctac cccgcagctc gtgtacccca
    20761 tcatgccgtc gtgactccag gttccgtagc tcaggcggcg cggggcccgg ctgtgggtga
    20821 aaacagcccc caggtaggga actcggattt gcccggcatg cgtatgaccg caaaggtaca
    20881 gctgaggccg gtaccgggaa gcctcccggt agatctcgtt ggaatgagcc acaaacacgg
    20941 aaaaggcccc cgaagggacc gaggcaaaag ccgcccccgg atcgtgacat cggtaatagt
    21001 gcgggtcgtc cacccccacg atccagacct cgtctccgcc tcgagggatt ctaacggcgt
    21061 cgttcaacaa aaacgacatg ccgcttttct cgaggggctc gaccagctcg atgcagtcgt
    21121 ggttgcccag gacccccagg atgccgtccc gggcgcgaat ctcgggcaac aagcgcctga
    21181 gcctcgaaag cgcctcggcg gaagacccgg aattctccat tcgaaagtcg cccccaagga
    21241 tgcacaggtc cgcgggaaga tcccttacca tggactgcac tttttccgtg agcccgtcca
    21301 ctccgtccag gtgcagatcc gtgaggaaca ggacggtgta gccgtcgaag gaggggggca
    21361 gatcccgaaa agtgaagacg acccttcggg atctcacccg aagcgcgttt tccttcccct
    21421 tttcatacag ccccagaacc cggaaggtgg tcttcatgag catgtggtaa tagacggcgt
    21481 cttcgaaatt gaagagggtg cggaacggat cgttttgaat ccggcaggaa cggagctcgc
    21541 gaaaccgaac gtcggggcgt acgggtcgcg ggtacccttc aaaaggcttg ctgagcaggg
    21601 tcctctgaag aagaaccgac cctccgtagg ctcccaaccc gaaaattccc aggtaaaccg
    21661 ccgcctgcag taggttcaat gaaaaatagg gagccagcac caggagcggc aggccgccct
    21721 cgaatacctg gtccagaccg ggcacaactc ttcccgcctc gaggccggct ctcctcttga
    21781 ggaagctcga gagcaggtcc ccccccatcc cgaggatccc gcaggacagg cccagccacc
    21841 agggaaaccc caaaggaagg cccacaacca ggcccgcaac cacaccgccc aggacccccc
    21901 tcgaagtctt gtgaggccca aacaaggctt cgccgtcacg aagaataagg ccccgatcca
    21961 aaggcgtatc ccaacgtcgg cggaggaaaa aggcaagaac caagggggtg agattgacgg
    22021 cccatcccaa tgtgaccatc ttgaccagga tgatgtcgaa aaagaacctc acgtcttcgg
    22081 accggcctcg ttgccgaatg ggaaccggag ggaaagcaag gcaattcgaa ggctccttgg
    22141 agcctccgga ttgcctttaa tcgatgaagg tcttgagagt ggactgccga tttgggtttc
    22201 gaagctttct cagggccttc ttctcaatct gtcgaattct ttccttggac actttaaacg
    22261 atcttccgat cttctccagg gtttccgccg gctgcccgtc aatcccgaag cgcaatctca
    22321 ggatcttttc ttcccgaggc tgcaaactgg acaggagtac ccgggtcatt tcggccagct
    22381 cccggtcgga acactcctcc atgggagaag ccgcccgatc atcctcgacg aaatccccca
    22441 gtcgctgctc atcgtctcca atgggcgttt ccagggaaat gggctgagaa gccagggtga
    22501 gcaccgtctg gactttctcg aggggcaact tggcccgctc ggcgatctcg tggggcatgg
    22561 gctcgcgtcc caactccttc aagagctcgt agaaaacctt gtagaaaaga ttcttcatct
    22621 cgataaaatg cacgggcaga cgaatggttc gagtcttgtc gtaaatcccc cggatgatgc
    22681 tctgtcggac ccaccaggtg gcgtaagtgc tgaaccgatt ccccttggtg tgatcgaatc
    22741 gtcccacggc cttgaggagc cccaggttcc cttcctggat cagatcatca aagctcattc
    22801 cccgcccccg gtatcgtttg gcgatactga gaaccaggcg caaattggcc ttcaccatct
    22861 cctgcttggc aagatcgatg gacgccagga tggaccgggt gcgccgcaga aggtcggaaa
    22921 acaagggtgt gttggaggag atttccgccc cgcgctcgag agtgcgaagg atgaccttga
    22981 ggatcttgtc cctcaccccc gggaaggttt tctcatggca gatgagcttt tcgatcttgc
    23041 cctgcaggtc cacgagggcc gaatcctcat ccgcgtgctc ttccaccatt cgtacgagtt
    23101 cctcttcccc ccggcggatg atttgagcca gctcgatttc cctttcctgc gtcagcaacg
    23161 ggtagccggc cacctccttg aggtagcagg tcacgggatc gtcttcgaaa tcttcggaca
    23221 gggccgctcc ccccgcgggc agatcttcct cttccacttc ctccccgcca tagcccgtct
    23281 cgtgcccggc ttcttcccgt tcctcttccg cttcgggcgt atccagggca agatccgttt
    23341 ccgcaaattc tccttccatc atcccctctt gaaacaggtt gtcgaaatca tcctgcagtt
    23401 ccgacttggt caagagaaga tccagaggga tgcagttttc cgaagagtat gtggtcattc
    23461 aaaccctcca atgcggggta catcctgaag cgatcttcct ccccccgctg ctttcctatt
    23521 ggttctccgc aggctttcgc atccttgccc cgaaggcaac ggaagggatc caccctgcgg
    23581 ttttccacgt aagatgataa gtacactcaa atacttagtc aattcttagg aataaggatc
    23641 cccggtaggc ccaaaccttc ggcgtgcttc ctggattctt ccagaaacct ttggatcacg
    23701 gttggtcgcg tgagaggagc tgcgtttccc gatatgtggc cgtcctataa atgaatctca
    23761 gcaatgtctc cgtcctgcag gacataatcc cgctgaacca tctgtccgtc aaagacccct
    23821 ttaccccata ctttggcaaa tttcaattta tttctgaagt ccttgtggat tttttccgcg
    23881 agatcctcta tagtggctcc tttggccaag atgaaaggcg ccgtcatgtc ggggtccttc
    23941 cctccgggct tggtgtaaac ccgaatgatc tgcgcctggt caaagaccat acggagaaaa
    24001 gcccccaggt tccgcgaagt ccgggtcgaa attcccacac aaggcagctt ccattccgag
    24061 agctccagga atgcccggta gtcttcctca tccccttcct catccacctt gttcaccgcc
    24121 accaggatct tcttgacgaa cggcattttg ggcaggtccc cgggaaccgg tcggcccagg
    24181 ggataaatcc gaagcccctc caggatcttc agtgtgtctt ccagctgctg cagggggtcc
    24241 gcatgcaggt ccacgaggat gaccaggatg tccgcacggc gcatcaagtc ggtcatgccc
    24301 ggatcgatcc gctccagggt aatggcggga gtatccacca gttggaactg gatgttttcg
    24361 aagggagcca tccccggggt cggtttccag gtggtgtgcg ggaactcggc cacctcgggg
    24421 caggcgttgc tcaagcacgc tacgagggag gactttccca cgttgggagc gcccaccacc
    24481 aacacttggg ccgctccctc ccgttcgatg gaaaaggtcg ccgccctcct ggctccgccc
    24541 ttcttctgct gggcctggtc cttgagtttc ccgatgcgac ggcgaaggtc cgcccgcagc
    24601 ttatccgtgc ccttgtgttt gggcatgatg gtaagcattt cttccagggc ctcgatcttc
    24661 ccttcggagg tcttggcttc ccgaaatcgt ttctccgctt cgtaatagtg cggcggcagg
    24721 ttcgcgggca tacgcgaatc cccctgctcc tttctctcca cagaatctat ggatcgttgc
    24781 ccccgggccg ttgaaacggg agcttcccgg ttgccaagcg tctgcaaagt ctatataagg
    24841 gtttcgtcgg tcgtcggccc cgcaagatcc ggacaaacaa aggagacaat gccttgacct
    24901 gcccaaagtg tgaaaaagag ttggacctcg ttcgaacctg acgtcgggtg tactggcact
    24961 gccgggactg cggtgccaca tatcccttat cggagctccg ccacaccctt tcggaggaaa
    25021 cggaggccga gctttcccac tgcagggtgg accggctgta gcgggccgag ccttttcccc
    25081 ctcgacgccc ttgccgtttt cgggagactc ccggcaggtt tcgttgcacc cggggggcga
    25141 tcccgaccta gagtctctcc tcgatcagtc tccgagcgag gtccagggca tcgtccagtt
    25201 tttccgggcg gtttcctccc gcctgggcca tgtcgggtct tccgccgccg ctgcccccca
    25261 cttcccggac gatctcccgg atgagttccc ccgcgtgaac ccgcttgacc aggtccgggg
    25321 taacccccac cagtagaaac accttcccct cgtggaccga tcccagcacc accaccccgc
    25381 tcttcaacct ttccttgaac cggtcgttca tttcccgcag gaccttggga ttgtccgcct
    25441 ccacccgggt gacgaggacg gtcgccgagc cgatctgccc ggcctccgcc agcaggtccg
    25501 cagagcgtcg ggcggcaaag gaagccttga gggattccag ctccttttcc atctgctttt
    25561 gagcattaag aagtcgctcg atccgatcgg ggagctcgac aaccccggtt ttgagcaaac
    25621 cggcggcttg gcgcagcacg gtctgctgct tttggacata ctcgagggcc cgtcttcccg
    25681 ccagggcctc gattctcctc accccggcgg cgattcccgt ctcctggagg atgacgaaaa
    25741 gccccacgtc tcccgtgcgg tgcgtgtggg tgcctccgca aagctctcgg ctgaggcccg
    25801 ggatctccac aagcctcacc tcctccccgt atttctcctc gaaaagcgcc atggcgcccg
    25861 tcttcatggc ctcttccaac cccatgaccc gaacttcgag gtcttgattc cggcggattt
    25921 ccaggttcac ccggcgttcg atctccgaaa tttcctcggg gctgagggcc gcaaaatggg
    25981 agaaatcgaa acgcaaacgg tcgggggcca ccagagaccc cgcctgcttc acgtggtctc
    26041 ccagcacctt gcgcaaaacc gcatgcagga tatgggtggc ggtatgatgc agcgccgtgt
    26101 cctgtcgcag ggcttcatcc accttcaacg tcacctcgtc ccccacgcgg accgaccctt
    26161 ccagaacttt tcccacgtgg acgaaaaggt ccccgggaag cctcatggta tccagaacct
    26221 cgatccgtcc cgaaggcccc aggatctcgc cccggtctcc cacctgtcct cccgtttcgc
    26281 cgtagaaggg ggtctcggag gtcaccagct cgatctcttc ccccgtttcc gcctcctgca
    26341 cggccgtctc gttcagaatg acggcctcca ccttagacca ggccaccagg gtttcgtacc
    26401 ctaagaagac cgttttcagg cccagagccg agagctgccg atacacttcg gcaatttccc
    26461 tttctccgct tcctttccag tgcagtcgag actgttttcg ctgcttttcc atgagttcct
    26521 ggaagcccgc ctcgtccacc cggaagtccc ggtccctcac cgtatcacta atgatatcaa
    26581 tcggaaaacc ataggtatcg tagagcttga agatgagaga acctggaatg gacttctctc
    26641 cttcgtcctg taaacgcctg acttcgctct ggagaagctt caaaccgaag tccagggtct
    26701 cgttgaaccg ttcctcttca tggagaatga cccgggtgat gaagctcttg ctctcgagca
    26761 gttccgggta agcgtcctcc atggaggtca tcaccgccac cgccatgtcg tggagaaacg
    26821 gacggtccag acccaggaaa cgcccatgcc gcaacgcccg gcggatcacc cggcgcagga
    26881 cgtaacccct gccctcgttg ccgggaagca ctccgtcatt gatgagaaag gccgccgccc
    26941 ggctgtggtc ggcgatgacc tttacggaca cgtccttttc cccatagctg ccgtaacggt
    27001 agccggtcaa ctcttcgatc cttgacatca tgggagagaa caggtccgtg tcgtaattgg
    27061 aagggactcc ctgttttacg gcggcgatgc gctcgaggcc catgccggtg tcgatgctgg
    27121 gtttgggaag gggttccagg ctcccgtctt ccttgcgatt gaactgcatg aagaccaggt
    27181 tccacagttc gagatagcgg tcgcagtcgc acccgggttt gcagtccggc ccgccgcatc
    27241 ccattttttc tccctggtcg atgatgatct ccgagcacgg tccgcaggga cccgtatcgc
    27301 ccatggccca gaaattgtcc ttggtgggga attcgaggat tcgatcctgg ggaagatacc
    27361 gggcccagaa gcccttggct tcctcatcgg gccccaggtg catggtcgca tctccctcgt
    27421 gcaccgtggc atagagcttg tccttgggca gtcccatccg gtccgtgaga aacgcccagg
    27481 cacactcgac ggcgtcccgt ttgaagtagt cgccgaagga gaagttgccc agcatttcga
    27541 aaaaggtgtg atgccgggcc gttctgccca cgttttccag gtcgttgtgc ttgccccccg
    27601 ccctcatgca cttctggctg ctcgccgccc ggctataggg tcgcttttcc tcacccagga
    27661 aggaccgctt gaactgaacc attccggcat tggtgaacaa cagggtcgga tcgtcgtggg
    27721 gaatgagcga tgaactcttg acgacggtgt gccccctgtc ccggaagaac tccaaaaaag
    27781 ccttacgaat ttccgtggcc ttcattccaa ggtctcctcg atcctcgatc ttcaatccgc
    27841 tgcaacggca tccggcccgc ccggggggat tttcaggttg tgagcctgac gggttttccg
    27901 ttcgatttcc aggaaaagat ccggatgttc tctcaaaaag cttttggcat tttcccgacc
    27961 ctgtcccaac ctctctccgc cgtaggaata ccaggtaccg gacttgtcga cgatccccgt
    28021 atccacggcc aagtccagga catccccttc cttggagatt ccgcggccgt agatgacgtc
    28081 aaactccact tccttgaagg gaggcgcaat cttgttcttg accaccttga cccgggtccg
    28141 gttgcccacc atctcctggc cctccttgat gggggccacc cgccggatat ccatgcgcat
    28201 ggtggcgtag aacttcaagg cgttgccccc cgtggtcgtc tcgggggacc cgtacatgac
    28261 gccgattttc atccggatct ggttgatgaa gatgacggag gtcctggact tgctgatgga
    28321 agagaccagc ttgcgcaggg cctggctcat gagcctcgcc tggaggccca cgtggggatc
    28381 tcccatttct ccctcgattt ccgccttggg aaccagggcc gccaccgaat ccaccacgat
    28441 gacgtccacg gcattgcttc gaaccaggat ttccgcgatc tccagggcct gttctccgta
    28501 gtccggctgg ctgatgagca ggtcctccac ctggaccccc agcttgcggg cgtaatggac
    28561 gtccagggca tgctcggcat cgatgaaagc ggccaatccc cccagtttct gcgcttccgc
    28621 gatgatgtga agggccagag tggtcttgcc cgaagactcg ggcccgaaga tttccacgat
    28681 ccgcccccgg gcgattcctc cgatgcccaa agccaggtcc agggaaagac agcctgtggg
    28741 aattacggca acttccacct ggagggcgcc ttctcccaat cgcatgatgg cgcccttgcc
    28801 gcaggagcgc tcaatctggg atatggccgt gtcgatggcc ttttgcctgt cgtcgttggc
    28861 tcccatgcgg gatctcctct tcatggtatg agtgtagcga tccttttccc ggaaagcaaa
    28921 aggggctcga gggctttgga tcttcacggg gttcagagga caaatggcag agcacggact
    28981 gcagggttgg attctttgcg gcatccaccc ccatttcctt ttccattccg agcgccggtt
    29041 ggaactcacc cccgggcaaa acccgcccgg tacaaaggag tgtagtgcgc gccgtctggt
    29101 ttcaggtcac ttttatacag tacaagatcc gagacgtcaa aagcttccgt ctcgaaggcc
    29161 aggttggcca gcaggatttc cgtgagttcc cggtttcccc tgggcccctt gaccctcccc
    29221 agggtcaggt gaggccgaaa gcgccggccc tcgggctcga aacccagggg agccagggcc
    29281 gcctccaccc gggatcggag ggtgctcagg ccctcgagat ctcccttcat ccccacccac
    29341 accacccgca tctgcttgag agaagggaaa gccccgcaac cccccggctg cagtcgaaac
    29401 ggtgaaacgg acgctgcggc cttccccaga gcgtccttga ttccgtcgat cctctccggg
    29461 gccacgttcc ccaagaactt gagggtcagg tgaatccgtt cccccttcac ccacgccacg
    29521 gcggcgccgc tttttcgaaa agcctcgatg agaccgccca gcgactgccg gacggtcatg
    29581 ggaaggtcaa gagccacaaa ggttcgaatc atcggacaaa accctccaca ggagcaccag
    29641 ggccgcctgg gcagcgcact cccggatgtg cccccggctt cccttgaagc gatgcttctc
    29701 cacttcacag cccaaggccg tcgaacaggc gatgaatacc gtacccacgg gcttctcggg
    29761 ggtccctccc gtgggcccgg caatccccgt gacggatacg gccacgtcgg tcccggcctt
    29821 cctcctggcc ccttcggcca tggcccgggc caccggttcg ctcacggccc caaggctccc
    29881 gatcagctcc gggtctaccc ccagcaagtc cactttggcc cggttgctgt aggtg
//
LOCUS       DAOOVC010000039        29690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1055_length_29690_cov_6.344525, whole genome shotgun sequence.
ACCESSION   DAOOVC010000039 DAOOVC010000000
VERSION     DAOOVC010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 29690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29690
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1055_length_29690_cov_6.344525"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..964)
                     /gene="nth"
                     /locus_tag="PLM79_07235"
     CDS             complement(287..964)
                     /gene="nth"
                     /locus_tag="PLM79_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOV86140.1"
                     /translation="MVPHNPSSRPPAGESLQGKAAAVLPLLDRMYPQARCSLNFENPL
                     ELLVATILSAQCTDERVNQVTPGLFRKYPTAGAYAQVPIEELEADIKSTGFYRNKAKS
                     IRSCCAILAERFEGAVPRDLDLLVQLPGIGRKTANVILGNAYGIPGIVVDTHVSRVAQ
                     RLGLTLQKDADKIERDLMALVPRERWTLFSHQMIQHGRNLCRARKPRTEACPLRPHCD
                     FGSGRST"
     gene            1251..1592
                     /locus_tag="PLM79_07240"
     CDS             1251..1592
                     /locus_tag="PLM79_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86141.1"
                     /translation="MKTHIIVCPFCENGQPVPTDFDSIHRCNCGACFKVCGNHALESS
                     VGDIAEQLWNEAELNFIRSIPLDFCNIVVEKNFERLLDLKQTFDLEGIEKFCKYDTNT
                     HLSLVWVKRLF"
     gene            complement(1650..2237)
                     /locus_tag="PLM79_07245"
     CDS             complement(1650..2237)
                     /locus_tag="PLM79_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOV86142.1"
                     /translation="MKLRYDLFCEVCVDKRGINLETLRHVITLAIEIAREGREGRRIG
                     TMFVISDLEQVLNHSRTLILDPLYGHPDHLKRIEDPDARETIKELSQLDGAFVVSDEG
                     VVVSACRYIDAFSEGINLPLGLGSRHMAAASITKQTNAVAVVVSESSVVRIFDDGELI
                     SQIIPELWMFSRYGSHLARSLDGAEGEVTVLTDPE"
     gene            complement(2221..5508)
                     /locus_tag="PLM79_07250"
     CDS             complement(2221..5508)
                     /locus_tag="PLM79_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV86143.1"
                     /translation="MKDRGPSHHWLSSIVEAFLQGNLSVLLILISLLAGIAALRITPR
                     EEDPQIVVPIADVLVSVPGASAREVERQVATRLEKLLYQIDGVEYVYSTSYPSQAILT
                     VRFYVGEDRERSWVKLHNKIAANIDQVPPGVAGWVVKPVEIDDVPIVNLTLFSNRYGD
                     YDLRRLAEELEIRLQGVPNAGRTYLVGGRSRQVCVVLSPQLMAGRGITIQDIQRAVRG
                     SNASLPAGELVAEDRELRFQAGRFLMSAREVENLVVGARDSRVVRLREVATVRDGPSE
                     PDTYTHIAFGKKAFTPMDPSAAAAPVETDVEPFRDYPAVTVAVAKRKGTNAVRVSEEV
                     RKEAERFAREVLPEGVRLRVTRDYGQTADHKVNELVEALLVAIAIVVVLLAYTLGWRE
                     GIIIALAVPVTFSFTLLLNYFLGYTINRVTLFALILALGLVVDDPIVDVENIHRHFSM
                     GRKDPFHAVIEAVNEVRPPIILATLAVIISFVPMFFITGMMGPYMAPMALNVPISMLM
                     SLLVAFTITPWLSLHMLRGGSRQPHSEPYSMESSPLYRLYERTLTPLMADARLRWGLL
                     AWVGVLLVFSGWLVVSQRVPLKMLPFDNKNELQVVVDTPEGTSLESTEAAAHELASYL
                     IRIPEATDVAVYTGTSSPVDFNGLVRHYYLRRGSNVADIRVNLVGKKYRVQQSHAVGL
                     RVRNDLHRIAQKHGANLKIVESPPGPPVISTLVAEIHGSIGQSYEELAAAARKVRSWM
                     EHTPGVVDVDDVLTAPQEKTVFVLDRTKASVHGISDHVLAEALQGVVSGDAPGSLRLD
                     REVYPAPIKLRFPREERSRTANLEQLMVRGEGGDRVFLSELGSFESRTIDQPIYHKNL
                     RPVVYVFGETAGVPPPQAVFELSDRVGKDPELKHVEVVWSGEGEWDITLRVFRDLGIA
                     FGVAVLGIYILLLYQTQSYLLPAIQLIALPLSVIGILPGFWLLNLFTAETVDGWSDPV
                     YFTATAMIGMIALAGIATRNSILLIEFIEERKKEGSPLERSLIEAGALRTRPIFLTSL
                     AAMLAAWPITLDPIFSGLAWALIFGIAVSTFFTLIVVPVVYFMAYGEDSAEDGKQRKR
                     TQHEASI"
     gene            complement(5505..6698)
                     /locus_tag="PLM79_07255"
     CDS             complement(5505..6698)
                     /locus_tag="PLM79_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV86144.1"
                     /translation="MLREVLKGIGVLAILIFLMMWLGGAFVSKVRPGPAAEKGEPVPV
                     KTRKVERREYPLVLDQVGTVRTRTEATISSKIMAQVEEIFVEEGDFVVGRDQSTMAPT
                     VLARLDDRELRARLDRAQSQAAAMGKAASAARAKAAAARAQAESARANRDRAVSDHRR
                     YEDLRQNRAATGRQLDHSRAQRDMAEAQFQAAQKEIRAAESDLENFLAQRDQAEASVL
                     EAETLLGHAVIEAPFTGQVIRKTVNKGDLAVPGRPLFVLDTPALPELHTYVSESLLPH
                     LQPGQEMEVVLGAGKTSCTGKIREIAPQSDPATRTVLVKATLAPQSFLVNGLFGRLRV
                     PCGRYSALVVPEAAVWEVGQLDLVRVLGEDGHPRRRLVRLGESRDGWIEVLSGLHENE
                     EVVLP"
     gene            complement(6712..8256)
                     /locus_tag="PLM79_07260"
     CDS             complement(6712..8256)
                     /locus_tag="PLM79_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV86145.1"
                     /translation="MGKGLGPGGGGLFRFSAWFLGALFLFFPGGCSQSRPVPTAPKAV
                     LEAVPEPAPSPDPSVLPGPSSNPTPSSHLTVEKAVEEALGASPELEQILHRVEAAGEQ
                     VRQAQAAFYPRLVLTEDFHGTDNPMYALMDLINQERFRLKMPGGKSTPGGFDPFSNLG
                     RQQNLASRIRGEWSLFEGGRHLHDRRAAAYGRDSVRAELGAARNRLVAKVMEVYFQWL
                     QAIAFIEVAEKAHEAARTDEELAVARLKADTVLPTEVTRMKARTAEVHGDLVSAGTSA
                     RKLQAAMERLLARPIVPREIPDPALVASLPFHPTATPVENPVGKALSQRPEVQAVDAV
                     IAAARERMKAETGNIFPKIGSTAHYEWNTEDLSRSVGSWFVGLSATWPLFEGGASVSR
                     IQEARLRLKEMEARGKQVALDIALEVRQAELSLQEAMEKILIADERRKWARQAMEEVR
                     HLYRNEMVTVDALLQSEVSWNRAEVSYVAALFEGKIAYALLRQSMGDFADWVEKEHGN
                     GSFSGK"
     gene            8790..10619
                     /locus_tag="PLM79_07265"
     CDS             8790..10619
                     /locus_tag="PLM79_07265"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOV86146.1"
                     /translation="MTHRGSSGNDLADSFVGTEKRRSLFEQAVRTLPISVCSTFFDKD
                     GKIAPATFRETETVLENADQGRITAAFPRTVAKNRFVVLEGVPSLSGVSISGRRVGVL
                     FSGGPAPGGHNVLAGLKAVLGKGNTLLGIRGGPKGLLNGRVVEVTSRDVRDILNTGGF
                     NFLGTDRTKIKSPQQFEQVRETVLQNRMDGLVIVGGDDSNTNAAFIAEYFETTGVKCN
                     VVGIPKTIDGDLAVGNLLPIPFGFDTATKIYSELVGNLTQDAASAVKYWHFVKLMGRT
                     ASKITLEVALQTKPAVALISEEIAEKNISLENIVDSIVQVITERRLRGLNYGVVLVPE
                     GLIEFIPEMRDLIRELNAVLAEYEKDIRDLPTLRNKLEYIYPLLTPQSAQLMASLPDE
                     IEEMLILDRDDHGNVKVSQIETEKLLIEKIRYKISEIKRHTDRYFGEGEGKFAATPEQ
                     IESIRRITFAAQSHFLGYEGRSAKPTTFDASFAFALGMTAGSLVLADKTGYMAAVTDF
                     HKGGRVLAIPLPGLITVEMRKGKEEFVIEKSLVKLSSPSFQVYAANRDKWAREDYFSS
                     PGPIQHWGPTSKQIPISVALDQDYPEFADFDLGKECRLDPDEI"
     gene            complement(10830..11069)
                     /locus_tag="PLM79_07270"
     CDS             complement(10830..11069)
                     /locus_tag="PLM79_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86147.1"
                     /translation="MFKLDDLEKICGKCKGSGFIQDWQWIQWWQENDAPPPEGHPLLK
                     VQEEIPCEDCNEVGFIPTPMGAALLEFISHFRGRK"
     gene            complement(11577..12800)
                     /locus_tag="PLM79_07275"
     CDS             complement(11577..12800)
                     /locus_tag="PLM79_07275"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOV86148.1"
                     /translation="MEDRKELSRRRFLKTVGALGTGSFLAATDMGGAAPLDPDTKTAG
                     PSRVPRRPFGKTGVEVSCLALGGMFDIPSNQLLMRQALNWGVSYWDTADCYEGGKSEA
                     GIGQFFGKYPEARKEVFLVSKSDSRDPEGMTKLLQRSLERMKTDYVDLYFIHGMKKIG
                     EINAETKAWAEKAKAEGKIRFFGFSTHSNMEELLPAAARLGWIDGVMLTYNFRLMHTD
                     KMKAAVDACVKAGIGLTAMKTQGGGSVSAESETELRMAGRFLKLGFTDKQAKLKAVWE
                     NPHIASICSQMPNLTILSSNVAAAMNRTKLSETDLDLLRRYAGETASSYCAGCSDLCE
                     SCTGDGVPIRDVMRYLMYYRSYGDRDLARRLFAELAPETRQSLLTLDFSAAEKRCPQR
                     LRIGRLMREAVSLLA"
     gene            complement(12875..14812)
                     /locus_tag="PLM79_07280"
     CDS             complement(12875..14812)
                     /locus_tag="PLM79_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86149.1"
                     /translation="MRIVTARRISQFFFLVLFLWFCVVSSYGTQWRQLRGWPVNWFME
                     LDPLLALGTVLTTRTLYSTLLWALATVLLTLVLGRFFCGWVCPFGTLHQFLGYVGKRR
                     RDPSSAAAENAYRGAQRVKYYLLFFLLSAGAGSLAAWGIRTVFSHSILPALLAVGTLV
                     LSVLVLFRFLGPRKKAAGLMLGALGFWTCLAFFFAVDRWIDGSLQTGLLDPIPLLYRS
                     VNLAILPLLDSTSQRIHADQRFYYGAWVIGVIFLGALLLNLKTPRFYCRFLCPLGALM
                     GFLSHFALWKIAKKSGDCNRCGLCETHCEGACNPSDTIRTHDCVLCMNCFDRCGRDLM
                     TYAARPSVSGEITVPDLSRRGLLAAFAMGVALPPMVRLSGGTQANWNPHGIRPPGSLP
                     EAEFLRRCTKCGQCMRICPTNIIQPAGWETGPEGLWTPVLNFRAGTSGCQLNCVACGL
                     VCPTAAVRPITPDEKLGRNAYAAAGPIRLGTAFVDRGRCLPWAMDKPCIVCQEVCPVS
                     PKAVFVRERFVAVRNGAMGVRRTEGFRVEIQGPALTPGRFSTGDFYLHVLEGPGRGLH
                     RIAGNGTDALELDSGGPRETLPAPGAPIEIHVRLQQPQVDPERCIGCGICEHECPVSG
                     LRAIRVTAENESRSKSRSFLL"
     gene            complement(14825..15826)
                     /locus_tag="PLM79_07285"
     CDS             complement(14825..15826)
                     /locus_tag="PLM79_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV86150.1"
                     /translation="MESADLPGEDRREFLRRLAGTGAVVLGTGLLGYGLYDPEGPGPT
                     QGNPPEESLTLPNYTMPEAGRRISIVYGEDRIRALQTAVTALGGIEAFIKKGDRVLIK
                     VNAAFAMPPVLGATTHPDLVRETARLCLQAGASYVAVTDNPINDPSSCFALTGIGEAV
                     RSAGAQLILPREKLFSSATLPGGSLIRNWPLLYEPLRGVNKLIGITPVKDHHRSGASM
                     VLKNWYGFLGGRRNVFHQNIHELIKELALLIRPTLVVLDGTTTMMTNGPTGGSLSDLK
                     RTHTLIAGTDPVAVDAFGATLLGRSPRDLPFLLKAEAAGAGTVDFESLQPLRTNLSG"
     gene            complement(15826..16815)
                     /locus_tag="PLM79_07290"
     CDS             complement(15826..16815)
                     /locus_tag="PLM79_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86151.1"
                     /translation="MKKMQHGREPYISASVLLVLLLIAGGTAVKQARFDPSLFAPTSP
                     EEGLPQSPLSSAPRDLPVPLPDGLVPMSPVETFTPETLSEKINGKAELYLSAGFRSLR
                     SQRFMEKERSESWMELYLYDMGDLRNAFAVFTAQRREDAREVPLTPFAYETANALFFV
                     HGRHYVEIVSAAEESPAVMAALAEGLVRQEPGREETLPELALFPSEHRVEGSWVLLAE
                     NVFGFEFMNRTFTAEYELPEGRITAFLSLRRDPEEARQLADGYVRFLLENGGKAVECA
                     DGLPGAHCITILDTLEMVFSSGRALAGVHQAEAFASATRVARSLYERIAGEAP"
     gene            17116..18195
                     /locus_tag="PLM79_07295"
     CDS             17116..18195
                     /locus_tag="PLM79_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOV86152.1"
                     /translation="MENGLSSPIQKISTLTEDATKRKKEEVFGWWGPSPEEARKTMVG
                     KSKGLRDKRTTIKEAVGRYIKDGANIAIGGFVNTRPPVAIVHEIIRKGAKDLTLSFQS
                     NSICPELLAGAMLLYPDHLSIRRAELAWWGYEVIGIAPLFRYLCNNGLIELDDYTNYG
                     MSARFKAAAMGIEFLPVRDHGGSDMELVNRGIMVRSPFTGSSSYLVPACYPDVGILNV
                     TAADMYGNCRIFGAHCTCPEIAMAACHTIVTAEQIIPNENIRTYPNLTEIPYTAVDAV
                     IEQRFASYPGACYGFYWFDMEHILMFRSASEEFRKTGKRDALKKYYDEFIFGCETHDD
                     FLSKLPYKTLKKIKDLDGGQPIILS"
     gene            18239..19021
                     /locus_tag="PLM79_07300"
     CDS             18239..19021
                     /locus_tag="PLM79_07300"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOV86153.1"
                     /translation="MPEYTPFEMIAYTGSRVLEDETIVFVGTGLPIIASIHAQLTHAP
                     GLGIIFEAGPLTPILEMGMPLSVGDTRACRKACYLKGLSSVFELTQRGHSDYAFIGGA
                     QIDMYGNVNSTFEGGTYTRAQTRFPGSGGAGAMAANCEKSIIIMALEPRRFVPQLDFV
                     TSIGFGDGSPDYRKKSGVMGSGPYRVITNEALFGFDEETKRMVLLEAAKGRTPEDIQK
                     KVSFDLLVSPDLKEMAEPSEEDLRLLREECDPDGYFLARKLK"
     gene            19373..20623
                     /locus_tag="PLM79_07305"
     CDS             19373..20623
                     /locus_tag="PLM79_07305"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV86154.1"
                     /translation="MGILNLAELKPDMVLASDLKARNGRFLLAKGTRLTTQHLRVLKI
                     WGVVEADIAGAESSEEGLEAAPEIDPAVWKRAETICRERFILTDLDHPVNQKLLRLSI
                     LRKAHELSGWRPHGVLQAEPSLPDEANGAGTEEGLSLLDPSQLLKEEVRLSTLPVILA
                     QIIETINKPNSSPKEIANVVSRDTTLSARLLRIVNSAFYGFPHKIDTLSRAVLIVGTK
                     QMSALAMGVKVINIFKSIPCDLIDMKSFLEHSIACGIVARILAGHRSITNTERLFVGG
                     LLHDIGRLVLYNHMPHEARSALLAAKAEKRLLNDLEDEMMPWNHGRIGGSLLKKWRLP
                     VSLETITRYHHAPTVAKDPTEPAIIHVADIIVNSLGIGSSGERLVPPLNQRAWGLLEL
                     SVNTLVLAVQQTDSQFQELRRFLL"
     gene            20645..22747
                     /locus_tag="PLM79_07310"
     CDS             20645..22747
                     /locus_tag="PLM79_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86155.1"
                     /translation="MRKRAAPKQTLKFYQERVNHLEELNRFTLDALEMAASVGDFQPN
                     ITRLEDISVILRETKARILRLIQFQAVAFFMVEPDSNDFTLACVEPEEFRSYVQEQVD
                     HLIENGTFSWSLMEKRPVILTSKNQQKQIILHVMATSSRIRGMFVGVMEKDKMDIPDI
                     SLYLLSIVLLNSSNAIETFQLYNMIREINKDLENEKEKYKYLFEAAPDGVEVLSSDGA
                     VIDCNETQRVMTGYLHEEIVGRKAEDFFSPTSKALFKTDPAGGRGDCFEREVELVCKD
                     GSVLPVWRKEKVILDGNRNFKGSVVYNHDLSAIRRTQQEKLNLEAQLRQAQKMEAIGT
                     LAGGIAHDFNNILGAILGYTEMVLYKLSQEDPLRPDLLQVFQAGHRAKELVRQILTFS
                     RQSEQNRNPLDIAPIVKETLKLLRASIPSTIEIRQEVGERIGRIMGDPTQVHQVVMNL
                     CTNAAYAMREAGGLLEVGLERVAFPQRASVPHPRLEPGVYVRLTVRDSGVGMEPATVE
                     RIFEPYFTTKPKGEGTGLGLSVVHGIVRGYGGVVTVESEPEEGSTFRVYFPQIDGESE
                     ELQEVQPSIPGGSERVMFVDDERSLTILGKKMLGHLGYEVVALTSSVEALEYFRSHRD
                     SVDLVITDLTMPHMTGDVLAREMMSIRPGIPIILCTGFSDSMSEDKARRMGLRDFITK
                     PLIMKDLARTIRRALDSV"
     gene            23158..23784
                     /gene="lexA"
                     /locus_tag="PLM79_07315"
     CDS             23158..23784
                     /gene="lexA"
                     /locus_tag="PLM79_07315"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOV86156.1"
                     /translation="MALTPAQERVYRFLKQYLQEHGCAPSYEEIRRHLGFRSLNAVSK
                     HLKQLEQRGVLSSPWGNRKRSLELLPLRTEAASIPFLGIVAAGIPIEAVEVPEMIEVP
                     ESFLGGGNNFALRVRGDSMIEEGIRDGDILIISKQSHAERGQTVVALVKGEATVKKFH
                     PYGPEVELMPANSQMKPIRVPAGDVEVVGIVVGLLRHYRSRSVRSVRE"
     gene            23806..24972
                     /locus_tag="PLM79_07320"
     CDS             23806..24972
                     /locus_tag="PLM79_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86157.1"
                     /translation="MERRIVCLEIPDFYAALEELRSPELRRVPLALAGPGPRALIQGV
                     NRLAREEGLREGMVLSRAQRSCRKLRVLPPDPLFYGEQHGKNLDELGRYSPLVEGTVP
                     GHYFVDLTGTRRLWGPSPDAAVILERRLEMQRGLKARVGLASSKLVSRVAAGCVIAGD
                     LGCVFPGGEVSFLDPLPVAALPGVGVQTTSRLAGFNIRRIGQLAALSVRDLTAVFGTM
                     GVRLSQLARGVDPTPVLPFRRIPKLEFSRRMDRDEIERERLEAVLFRLVEDAGWALRT
                     RNRHPGKVALEVRYADGTTARRQHTLGPPARQVDRQWFRILRPTLSRLLERRVAVRRM
                     RLELWELVMPLRQMELFPWEEVPVGESPRLQEALDRVRQRFGRQAVFWGLGRTG"
     gene            24972..28211
                     /locus_tag="PLM79_07325"
     CDS             24972..28211
                     /locus_tag="PLM79_07325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOV86158.1"
                     /translation="MGPFVHLHVHSHYSLMRGVSSVEALCRAGAEAGFSFLALTDTNG
                     FYGLVHFLDAARRRRLRPLVGVCLEAPGESAVFLAKTPRGYELLCELVSRRHLEAGFS
                     LASHVPEAPRDLALLSADPRLLRVLSPRVQCWVEVLPGPCGRKALEISRSLGIPPVAS
                     NAVHFARPEDHSLHRLVRAIHLNRTLSTLPVEEVVQPDRWLKSAGEMARHFPHCPEAL
                     ANTQRLARQCHTAWGDFPAVFPHYRDREADHFAILVQKCRRGIARRYGESNPAVEERL
                     EEELGLLRSKGYVDYFLVVEDIVRRRPIHCGRGSAAASLVSYLLGITHVDPIRHGLLF
                     GRFLNPHRRDHPDIDVDFPWDERDGLWEELVRFYGPQRLAAVSNHVGFGARAALREVA
                     KVYGVPAAEIKNMTRRMSSWSRPGEMRDRVDTHPKFRGLVPEPPWPEIMQLAARLESV
                     PRHLSVHCGGIVLAPDRVSRYVPVQRSAKGVRVIQWEKDQAERAGLVKIDLLGNRSLA
                     VIRDALESIRENTGKELDYAGLNPIEDPATRDLLARGATMGVFYVESPAMRQLQQKTG
                     RGDFEHLTLHSSIIRPAANRYIREYIRRLHGAPYEPLHPRLEELLAENYGILAYQEDV
                     VQVARILAGFDWDLADGLRKVLSKKSPEQISAYRKRFYEGCARENIGRDVVDAVWDMI
                     ISFAGYSFCKPHSASYALVSFKSAYLKAHYPAEFMAGVLSNGGGYYSTRAYISEARRM
                     GLTVLGPDVNHSRWTYRGKGRVVRMGLQQLKGVRREALEKILDERERNGPFGSLEDFL
                     RRTDPAPAEGAVLVGSGALDALARGTLPPFEKAFGAHLDPVSPVGSGVRETRETPGSG
                     FQGGGGPRFSGTVPTRPQILWMVEATLRGRFSGGAGGTIGKNLSIPRRSFTGGVPPLP
                     DYTPEQRWEQEKETLGFVLSVHPLALFESALKRLPFPVVPAADLDRHVGRDVWVLGWP
                     ITRKEVLTRDGESMEFVSLEDQTAIYETVFFPDAFRRFCRDLDYDRAYVLYGRVESEF
                     GAVSLTVRKLRRLSLPDPHGSTSRPSEHRPPRSPSGSSAETRCPWARPC"
     gene            complement(28123..28905)
                     /gene="budA"
                     /locus_tag="PLM79_07330"
     CDS             complement(28123..28905)
                     /gene="budA"
                     /locus_tag="PLM79_07330"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOV86159.1"
                     /translation="MNRFRSLCVLCLLATTLLSACAHPQPPKDTVFQASTINALMEGV
                     YDGETTFGELRKYGDTGLGTFNALDGEMIALHSRFFQVKSDGTAYPVEDSSRTPFAIM
                     TFFDPDRAFSLEEPLSYKELEERLDRTLPTRNFCYAFRITGSFAMVRTRSVPRQTPPY
                     PRLVEVVKTQPSFDLQNVRGTLIGFRFPEYLRGVNVPGYHFHFLTEDAGRGGHVLDCR
                     LDRVKVEIDDLYRFNMALPTDSGFQRTNLTGTSAADVQKVEK"
     gene            29461..>29690
                     /locus_tag="PLM79_07335"
     CDS             29461..>29690
                     /locus_tag="PLM79_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase"
                     /protein_id="HOV86160.1"
                     /translation="MKENSSRVVMEADEIQRVLERLARGIMEKSQDSQKLALVGIHTG
                     GVHLARRIQSVIREKFALSVPVGSLDINLYRD"
BASE COUNT         5795 a         8951 c         9289 g         5655 t
ORIGIN      
        1 catgacgccc gtaccagctt gcgaagcgcc tggtggacga ggggcttcgc ctgcgtgagc
       61 gcatgcgttc ccttctcgat cgggtaccac tcgacgagcg gataaccagc catgcgaaac
      121 gcgtcgatcg tgtccttgcc cgccgccatc gttgtctcct acagtgacgc cccccccggc
      181 cggccggtcc gggtggaatg gagactgggg gcgacccagg ggagtctcat gaccgtaaaa
      241 gacgaggaga atgcgtcacg ggaattcctc gtttgaccgg gagggtttac gtggaacgtc
      301 ccgagccgaa atcgcagtga gggcgaaggg ggcatgcctc ggtcctgggt tttcgagccc
      361 ggcaaaggtt tcgtccgtgt tggatcatct ggtgagagaa cagcgtccag cgttcccggg
      421 gcaccagggc catgaggtcc cgttcgatct tgtcggcgtc cttttggagg gtgagaccca
      481 ggcgctgggc gacccggctc acgtgggtgt ccaccacgat gcccggaatc ccgtaggcgt
      541 tgccaaggat cacattggcg gtctttctcc cgatcccggg aagctgcacc aacaggtcca
      601 ggtcccgggg caccgcgcct tcgaaccgct cggcaagaat ggcgcaacag ctccggatgc
      661 tcttggcctt gttccggtag aaccccgtgg acttgatgtc ggcttcgagt tcctcgatgg
      721 gaacttgggc ataggccccg gccgtcgggt acttgcggaa aaggccgggg gtcacctggt
      781 ttacccgctc gtcggtgcat tgggcggaca ggatggttgc tacgagaagc tccaggggat
      841 tttcgaaatt gagagagcat cgtgcctgcg ggtacatccg gtccagcaat ggaaggaccg
      901 cggccgcttt tccctggagg gactccccgg cgggcggccg ggaggacggg ttatggggca
      961 ccatgagggc aaatcctctc tagaagcggt ttacgggacg gttggagtgg tcgtatcgga
     1021 agacgagggt tctcctatgg caaacttccg gttggatttc aaggggaaac gaattatcat
     1081 agggaaacgg tccccggcgg tgcgggattt gggctccgga tttcgggtaa aggaagcggt
     1141 ggttgaggat ggatttccct tcggagttgt cacccatgag cttgatggac gatgcgggca
     1201 gaaagcagtt cagtgcactc acttcagcga tccgaggtga aaggcgttcg atgaaaaccc
     1261 acattatcgt ttgtcctttc tgtgaaaacg gtcaaccggt tcccaccgac ttcgactcga
     1321 ttcaccgctg caattgcggg gcgtgcttca aggtttgcgg caaccatgcc ctggaaagca
     1381 gtgtggggga cattgcggaa cagctgtgga acgaggcgga gttgaatttc attcgttcca
     1441 ttcccctgga cttctgcaat atcgtggtgg agaagaactt cgagcgattg ttggacttga
     1501 agcagacctt cgatctggaa ggcatcgaga agttttgcaa gtatgacacc aacacccatc
     1561 taagcctggt ctgggtgaaa aggcttttct gatccccctt cggactcatc ccgcgtccgt
     1621 ccacggcagg cagtgtgcga ttccggcctt cattcggggt ccgtcaggac cgtgacttcc
     1681 ccttccgcac cgtccagcga ccttgccagg tgagaaccgt atcggctgaa catccacagc
     1741 tccgggatga tctgggagat cagctctccg tcgtcgaaga tcctcaccac ggaactttcc
     1801 gataccacca cggccacggc gttggtctgc ttggtgatgg acgctgcggc catgtgcctg
     1861 cttcccagcc ccaggggcag gttgatcccc tcggaaaacg cgtcgatgta ccgacatgcg
     1921 gaaacgacga ctccctcgtc ggaaaccacg aaggccccgt ccagttggga cagttccttg
     1981 atggtttccc gggcatccgg gtcctcgatt cgtttgaggt gatcggggtg gccgtacaag
     2041 gggtccagga tcagggttct cgagtggttg agaacctgct ccaaatccga aatgacaaac
     2101 atggttccga tgcggcggcc ttcccggcct tcccgggcga tttcaatagc cagggtgatc
     2161 acgtgcctca aggtttccag gttgattccc cgcttatcga cacagacctc gcaaaacagg
     2221 tcatatcgaa gcttcatgct gtgttctctt cctttgtttc ccgtcctccg ccgagtcctc
     2281 tccgtatgcc atgaagtaga ctacgggaac gacgatcaag gtaaaaaacg tggagacggc
     2341 aatgccgaag atcaaggccc acgccaagcc cgaaaagatc gggtccaggg tgatgggcca
     2401 ggccgccagc atggctgcca gagaagtcag aaagatcggc cgggtcctca aagcccccgc
     2461 ttcaatgagg gaacgctcca gggggctgcc ctccttcttc cgctcttcga tgaactcgat
     2521 gagaaggatg gagttccggg tggcaatccc agccagagcg atcatgccga tcatagcggt
     2581 agcagtaaaa taaaccggat cggaccaacc gtccaccgtt tcggcggtaa aaaggttcag
     2641 gagccagaaa cccggcagga tcccgatgac ggaaaggggc aaggcgatga gctggatggc
     2701 gggcagcagg tagctctggg tctgatagag caggaggatg taaatcccca gcaccgccac
     2761 tccaaaggcg atccccaggt cgcggaacac ccgcagggtg atgtcccatt ccccttcccc
     2821 ggaccagacg acttccacgt gcttgagttc cgggtccttg ccgacccggt cgctaagttc
     2881 gaaaaccgcc tggggaggcg ggaccccggc ggtttccccg aacacgtaga ccaccgggcg
     2941 caggttcttg tgatagatgg gttgatcgat ggtccgggac tcgaagcttc ccagttccga
     3001 tagaaacacc cggtctccgc cctccccccg gaccatgagc tgttcaaggt tggcggtccg
     3061 ggaccgttct tcccggggga aacgaagctt gatgggcgcc ggatacacct cccgatccag
     3121 cctcaaggac ccgggtgcgt ccccggaaac gactccttga agagcctccg ccaacacatg
     3181 atcggaaatg ccgtggacgg aggccttggt gcggtcgagc acaaagacgg tcttttcctg
     3241 gggagcggtg agaacgtcgt ccacgtccac cactccggga gtatgttcca tccaggagcg
     3301 cacctttcgg gccgccgcgg ccagctcctc gtacgactgc ccgatgctgc cgtggatttc
     3361 ggccaccagg gtggagatga cggggggacc cggggggctt tccacgatct tgaggttggc
     3421 cccgtgtttt tgggcgatgc gatgcagatc gtttcgaacc cgcaatccca cggcatggct
     3481 ctgttgaacc cggtacttct tccccacgag gttcacccgg atgtcggcca cattggaacc
     3541 ccgcctcagg tagtagtgtc gaaccagccc gttgaaatcc acggggctcg atgtccccgt
     3601 gtagacggcc acatcggtgg cctcgggaat tcgaatcagg taggacgcca gctcatgggc
     3661 ggccgcttcg gtggactcga gggacgtgcc ttcgggagtg tcgacgacca cctggagctc
     3721 gttcttgttg tcgaagggca gcatcttgag cgggaccctc tgggagacca ccagccatcc
     3781 cgagaagacc agcagcactc ccacccatgc cagcaacccc cagcgcaagc gggcatcggc
     3841 catgaggggg gtgagggttc gttcgtagag ccggtacagg gggctcgatt ccatggaata
     3901 gggttcggaa tggggctgcc gggacccccc ccggagcatg tggagggaaa gccagggagt
     3961 gatggtgaag gccacaagga ggctcatgag catggagatg gggacgttca gggccatggg
     4021 ggccatgtag ggccccatca tccccgtaat gaaaaacatg ggaacgaagg aaatgatgac
     4081 ggccagggtg gccagaatga tggggggacg gacctcgttc acggcctcga tgacggcgtg
     4141 gaaggggtcc tttcttccca tggagaaatg gcgatggatg ttttccacgt ccacgatggg
     4201 atcgtccacc accaggccca atgccaggat cagggcgaac agggtcaccc ggttgatggt
     4261 gtatcccagg aagtaattga gaagcagggt gaagctgaac gtgacgggaa ccgccagggc
     4321 aatgatgatt ccttcccgcc agcccagggt gtaggccagc agaacgacca cgatggcgat
     4381 ggccaccagc aacgcctcca ccagttcgtt gaccttgtgg tccgccgttt gcccgtagtc
     4441 ccgggtcacc cgcaaacgaa ctccctcggg cagtacttcc cgggcgaaac gttccgcttc
     4501 ctttcggact tcttcggaca cccggacggc gttggtgcct ttccgcttgg ccacggcaac
     4561 ggtgacggcg ggatagtccc gaaagggctc gacgtcggtc tcgaccggcg ctgccgctgc
     4621 cgatgggtcc atgggggtga aggccttttt gccgaaggcg atatgggtgt aggtatcggg
     4681 ctccgagggg ccgtcccgaa ccgtggcgac ctctcggagg cgcaccaccc gtgagtcccg
     4741 ggccccgacg acgaggtttt ccacctcccg ggcactcatg agaaatcttc cggcctggaa
     4801 ccggagctcc cggtcttccg ccaccagctc ccccgcggga agggacgcgt tggaacctcg
     4861 aacggcccgc tggatgtcct ggatggtgat tcctcgaccg gccatgagct ggggggacaa
     4921 cacgacgcac acctggcggc tgcggccccc caccaggtag gtccttccgg cgttgggcac
     4981 cccctgcagg cggatctcga gctcttcggc caggcggcgc agatcgtaat cgccgtaacg
     5041 gttgctgaag agggtgaggt tcacaatggg aacgtcgtcg atctccacgg gtttgacgac
     5101 ccatccggcg accccgggag gcacctggtc gatattggcg gcgatcttgt tgtgaagctt
     5161 cacccagctg cgctcccggt cctcgcccac gtaaaaccga acggtgagga tggcctggga
     5221 agggtagctg gtggaataga cgtactcgac cccgtcgatc tggtagagga gcttttccag
     5281 gcgagtggcc acctgccgtt ccacctcccg ggccgaagcg ccgggaacgc tcacgagaac
     5341 gtcggcgatg ggcaccacaa tctgaggatc ttcctcccgg ggggtgatcc ggagggcggc
     5401 gatcccggcc agcaggctga tgaggataag aagcaccgag aggttgccct ggagaaaggc
     5461 ttcaacgatg gagctgagcc agtggtgaga ggggccgcgg tctttcatgg aagaacgacc
     5521 tcctcatttt catggagacc ggagagcacc tcgatccagc cgtcacggct ttctcccagc
     5581 cgcaccagtc gacgccgggg gtggccgtcc tctcccagga ccctgaccag gtccaactgt
     5641 cccacctccc acaccgcggc ttcggggacc actagggcag agtatcggcc gcacggaacg
     5701 cgaagccttc cgaaaagacc atttaccagg aacgattggg gagccagggt tgccttcacc
     5761 aggacggttc gggtggccgg gtcggattgg ggagcgatct cacgaatctt ccccgtgcag
     5821 ctcgtcttcc cggcacccag taccacctcc atctcctgcc ccggctgaag atgagggagg
     5881 agagactcgg agacgtaagt gtggagctcg gggagggccg gggtgtccaa gacgaagagg
     5941 ggccggccgg gcacggccag gtcgcccttg ttcaccgtct tgcggattac ctggccggtg
     6001 aagggagctt cgatcaccgc gtggcccagc agggtctcgg cttccaggac ggaagcctcc
     6061 gcctggtccc gttgggccag gaagttttcg agatcgctct cggctgcccg gatctccttt
     6121 tgagccgcct gaaactgggc ttcggccatg tccctttggg cccgggagtg atccaactgc
     6181 cggcccgtgg cggcccggtt ttgccggaga tcttcgtatc gacggtggtc ggaaaccgcc
     6241 ctgtcgcgat tggcccgggc cgactcggcc tgcgcccggg ccgccgccgc cttggcccgg
     6301 gcggccgagg ccgcctttcc catggcggcc gcctgggact gggcccgatc cagtcgagcc
     6361 cggagctccc gatcgtccag gcgggccaga accgtgggag ccatggtcga ctggtcccga
     6421 cccacgacga aatctccttc ctccacgaaa atttcctcca cctgggccat gattttcgag
     6481 gaaatggtcg cctcggtccg ggtgcggacg gtcccaacct ggtccaggac cagcggatat
     6541 tcgcgccgtt ccacctttcg ggtttttacg gggaccggct cccctttttc cgccgccggg
     6601 ccgggccgga ccttcgagac gaaagcaccc cccagccaca tcatgagaaa aatcaggatt
     6661 gccaggaccc caattccctt gaggacttcc cgtagcacaa cgctttcccc cttactttcc
     6721 cgagaaggac ccgttcccat gttctttctc cacccagtcg gcaaaatccc ccatggattg
     6781 tctcaataag gcataggcaa tcttgccctc gaagagggcc gccacatagg acacctccgc
     6841 ccggttccag gaaacctcgg actggagcag ggcgtccacg gtaaccattt cgtttcggta
     6901 caggtgtcga acctcttcca tggcctgccg ggcccacttt cgtctctcat cggcgatcag
     6961 gatcttttcc atggcttcct ggagggagag ctccgcctgg cggacttcga gggcaatgtc
     7021 cagggccacc tgttttcccc gggcttccat ctccttcaag cgaaggcgcg cctcctggat
     7081 ccgggatacg gaggcacctc cctcgaaaag gggccacgtg gccgaaagcc ccacaaacca
     7141 actccccacg ctgcggctca ggtcttcggt attccactcg taatgagcgg tggagccgat
     7201 tttgggaaag atgtttcccg tctcggcctt catgcgctcc cgggcggccg cgatgaccgc
     7261 atccaccgcc tggacctcgg gcctttggga cagggccttt cctacggggt tttccaccgg
     7321 cgttgcggtc gggtggaagg gcagggaggc gaccagggcg ggatcgggga tttcccgggg
     7381 gacgatgggt cttgccagca gccgttccat ggcggcctgg agcttgcggg cgctggtccc
     7441 ggcgctcacc aggtccccgt gaacctcggc ggtgcgggct ttcatccgcg tcacttccgt
     7501 ggggaggacc gtgtccgcct tgagccgggc gacggccagt tcctcgtccg tgcgggccgc
     7561 ctcatgggcc ttttcggcca cctcgatgaa agcgatcgcc tgaagccact ggaagtacac
     7621 ttccatgact ttggccacca accggttgcg ggcggctccc agctcggctc tcaccgagtc
     7681 ccgtccatag gcggccgccc gtcgatcatg gaggtgcctt cctccttcaa acagggacca
     7741 ttctcctcga atgcgggagg ccaggttctg ttgccgtccc aggttcgaaa aggggtcgaa
     7801 tcccccagga gtggatttac ccccgggcat cttgagtcga aaacgttcct ggttgatcag
     7861 gtccatgagg gcatacatgg gattgtccgt cccgtgaaag tcctcggtga ggaccagccg
     7921 agggtagaac gcggcctggg cttggcggac ctgttccccg gccgcctcca cgcgatggag
     7981 gatctgctcg agctcggggc tggcccccag ggcctcctcg acggccttct ccacggtaag
     8041 atgggaagag ggggtcgggt tcgaagacgg gccgggcagg acagacggat ccgggctcgg
     8101 ggcaggctcg gggaccgcct cgaggaccgc cttgggcgcc gtgggcacgg ggcgggactg
     8161 tgaacatccg ccggggaaaa acaagaaaag ggccccgaga aaccatgccg aaaaccgaaa
     8221 cagacctccg cctcccgggc caagcccttt gcccatttcc tccccctccc cgggagtccc
     8281 gaaagacccg gatgacccaa cccctccccg gggtctcgat ggtttccaaa cggagcgatt
     8341 ccaatgcacg gggcaaacaa aagccgggct cggtgcaggg gggggaaacc ccggcccgcg
     8401 tcccctgcgg aggtccctga aacgaacgaa ccgctcaggc ggctgcccga cctacggctg
     8461 cccgacataa cgaccgagag taccccagaa ccggcagaga gtgaagagcc ttatcccgga
     8521 agggagggcc gagcgcgaga gctcgatccg acctttccgt tgcaggatcg gcacaagggg
     8581 gaatcgggca cgggaagatc tttgcagttg aatttgttcc tcacatctgt ttccataaat
     8641 tccggatcgt ccgcttgccc atgggccgac gattgcatcg accgccttcc ggagacccgt
     8701 gccccctgcc tccctcttgt cccggggaga ctcccaactc agaggccgga cctccgggac
     8761 gagaccatct ttcggggagg tatacgaaaa tgacgcaccg tggatcatcg ggaaacgatc
     8821 tagccgacag tttcgtcgga accgaaaaac gacgttccct tttcgagcag gccgtacgca
     8881 ccctgcccat ttccgtttgt tccacctttt tcgacaagga cggcaagatt gcccccgcca
     8941 cctttcgaga aaccgagacg gtcctggaga acgcggacca ggggcggatt accgccgctt
     9001 ttcccaggac cgtcgccaag aaccgctttg tggtgttgga aggagtgccc agcctgtcgg
     9061 gggtgtccat ttccgggaga agagtgggcg tcctgttctc ggggggcccg gctcccggag
     9121 gacacaacgt cctggccggt ctcaaggccg tcctgggcaa gggcaacacc ctgctgggta
     9181 tccgaggggg ccccaagggg ctcctcaacg gcagggtagt ggaagtcact tcccgggatg
     9241 ttcgggatat tctcaacacg ggcggcttca atttcctggg cacggaccgc accaagatca
     9301 agagccccca gcagttcgag caggtgaggg aaacggtcct tcagaaccgg atggacggcc
     9361 tggtcatcgt cgggggagac gactcgaaca ccaatgccgc cttcattgcc gagtactttg
     9421 aaacgacggg ggtgaaatgc aacgtcgtag gaattcccaa gaccattgac ggggatctgg
     9481 ccgtggggaa ccttttgccc atccccttcg gtttcgacac cgccaccaag atctattcgg
     9541 agctggtggg caacctgacc caggacgccg cttccgcggt aaagtattgg cacttcgtga
     9601 agctcatggg acgtaccgcc agcaagatca ccctcgaagt ggccctccag accaaaccgg
     9661 cggtggccct catctccgaa gaaattgccg aaaagaacat ctccctggaa aacattgtgg
     9721 attccatcgt ccaagtcatc accgaacgcc gcctgagggg gctcaattac ggggtggtcc
     9781 tggttcccga ggggctcatc gagttcatcc ccgagatgag ggaccttatc agggagctca
     9841 acgcggttct cgccgaatat gaaaaggaca ttcgggacct tcccaccctc cggaacaagc
     9901 tcgaatacat ttaccccctc ctcactcccc aatcggctca gctcatggcg tcccttcccg
     9961 acgagatcga ggagatgctc atcctggacc gagacgacca cggcaacgtg aaagtctccc
    10021 agatcgaaac ggaaaagctc ctcatcgaga agattcgcta caagatttcc gagatcaagc
    10081 gccacacgga ccggtatttc ggggaaggag agggcaagtt cgccgcgaca cccgagcaga
    10141 tcgaaagcat ccggaggatc acctttgcgg cccagtcgca cttcctcgga tatgagggac
    10201 gaagcgccaa accgacgacc ttcgacgcgt ctttcgcctt tgcactggga atgaccgccg
    10261 ggtccctggt gctggcggac aagaccgggt acatggctgc ggtgacggac tttcacaagg
    10321 gcgggagggt gttggccatt cccctgcccg gactgatcac cgtcgagatg cgcaagggca
    10381 aggaggaatt cgtcatcgag aagtccctgg tgaagctgtc ctccccgtct ttccaggtct
    10441 acgccgcaaa ccgggacaag tgggccagag aagactattt cagcagtccc ggacccattc
    10501 agcactgggg tcccaccagc aagcagatcc ccatctccgt ggccctggac caggactacc
    10561 cggaatttgc cgacttcgat ctgggcaagg aatgtcggct ggatccggac gagatctgaa
    10621 ccgagaagtg cggattcgtc cctttcccgg acctgccgag gagaaaacgc tgcggagtcg
    10681 tttcccggct cgaatcccgg acggcgatct ggacggaagg acccgcccgg tgcatttctt
    10741 tgattcgaag gggttcccgg ttcagagagg gggcatttgt atggtgccgg agaggtgcgg
    10801 cccccatggg aacccctcga tgaacccctc tactttcttc cccgaaagtg actgatgaat
    10861 tcgagcaatg ccgctcccat gggggtggga atgaacccga cctcgttgca gtcctcgcac
    10921 gggatctcct cctgcacctt caacaggggg tgtccttcgg gcgggggggc gtcgttttcc
    10981 tgccaccact gaatccattg ccagtcctgg ataaaaccgg aacctttgca ctttccgcag
    11041 atcttctcga gatcatccag cttaaacatg aaggcctccc gataaagaca aatgctgccc
    11101 aaagccgccg ggtccccggc accctttcgg atcaaagcgc cgtccgattt ccgcccagat
    11161 tgagcgattg agctggtccg aaccccgttt cgtccttcct gcggatgaga gacctccatt
    11221 gaacggggcg caacccgttg cacggacgat tcccgcgcag gcgggaatcc ggaaagagcg
    11281 tacatgcccg ttggatgccc gccttcgcgg gcatggcgga cggatgctcc cggaatcgct
    11341 ccacccggtt ttcatatacc agctttccga ccctgcaaac aggaaaaccc ggactcttcc
    11401 gatccggaca gaggtgcctc ttttccggga aaaggcccgc ccggtccctt cgaaaaaatc
    11461 ggtcctcctc cgggccgtcg agatccatgg gggccctcag aagatgcgat ccccgcctcc
    11521 ggcctcgatc ccgacaggtc tcctcccggt ccgcgtgctt cgcgaacttg cagacactag
    11581 gcaagcaggg aaacggcctc gcgcatgaga cggccgatcc gcagcctctg ggggcatcgc
    11641 ttctcggccg ccgaaaaatc cagggtcaaa agggactgcc gggtttcggg cgccagttcc
    11701 gcaaacaacc gccgggcaag gtcccggtct ccgtagctcc gatagtacat gagatagcgc
    11761 atcacgtccc gaatggggac gccgtccccg gtgcaggatt cgcaaaggtc cgagcacccg
    11821 gcgcaatagg aggaagcggt ttcccccgcg taccttcgga gaagatcgag gtccgtctcg
    11881 gacagcttgg tccggttcat ggccgccgcg acgttggagg aaaggatggt caggttgggc
    11941 atctgggagc agatgctggc gatgtggggg ttttcccaga cggccttgag ctttgcctgc
    12001 ttgtccgtga accccagctt caggaaacga ccggccatcc ggagctccgt ttcggactcc
    12061 gcgcttacgg accctccccc ctgggtcttc atggccgtta ggccgatccc cgccttgacg
    12121 cacgcatcca ccgccgcctt catcttgtcc gtgtgcatga gtcgaaaatt gtaggtcaac
    12181 atgaccccgt cgatccaacc cagtcgagcc gccgcgggca ggagctcttc catgttgctg
    12241 tgagtgctga agccgaagaa ccggatcttg ccttccgcct tggctttttc cgcccaggcc
    12301 ttggtttcgg cgttgatctc accgatcttc ttcatgccgt ggatgaaata aaggtccacg
    12361 taatcggtct tcatgcgttc gagggaacgc tggagcagct tggtcatgcc ctcggggtcc
    12421 cgggaatcgg acttggaaac caggaagacc tcttttcgag cttccgggta ttttccgaag
    12481 aactgcccga tcccggcctc gctctttccc ccctcgtagc aatcggccgt atcccagtaa
    12541 gagacgcccc agttcaaagc ctgcctcatg aggagctggt tcgagggaat gtcgaacatc
    12601 ccccccaggg ccaggcagga cacctcgacc cctgttttgc cgaaaggtcg tcttggaact
    12661 cgggacggcc cggcggtttt tgtgtcgggg tccaagggcg cggcgcctcc catgtccgtt
    12721 gcggccagga aggagcccgt tcccaaggct cccacggtct tgagaaaacg gcgacgagac
    12781 agctctttgc gatcctccat gggtgagtcc ctttctctgg ttggttgagg gtgcgtggtt
    12841 gcagcgcagg cctgcacccg agacggggtt tggattagag caggaaggag cggctcttgc
    12901 tccgggattc gttttccgcc gtaacccgga tggcccgaag gccgctcacg ggacattcgt
    12961 gttcgcagat gccgcacccg atacagcgct ccgggtccac ctggggctgc tggaggcgaa
    13021 cgtggatctc gatgggagcg ccgggggccg ggagggtctc ccggggtccg ccagagtcca
    13081 gctcgagggc gtccgtcccg tttccggcaa tgcggtgcag cccccggccg ggcccctcga
    13141 ggacgtgcag gtagaagtcg cccgtggaga atcggcccgg cgtaagggcc ggtccctgga
    13201 tctccacccg aaagccctcc gtgcggcgaa cccccatggc cccgtttctc acggccacga
    13261 accgctcccg aacaaaaacg gccttgggac tcacgggaca aacctcctgg cagacaatgc
    13321 acggtttgtc catggcccag gggagacatc ggccccggtc cacgaaagcg gttcccagcc
    13381 ggatggggcc tgctgcggcg taggcgtttc ggcccagctt ctcgtccggg gtgatgggac
    13441 ggacggccgc ggtggggcaa acgagaccgc aggcaacgca gttcagctgg cagccgctcg
    13501 tacctgcccg gaagttcaat accggggtcc acaagccttc gggaccggtc tcccagcccg
    13561 ccggctgaat gatgttggtg ggacaaatcc tcatgcactg cccgcacttg gtgcaccggc
    13621 gcaggaattc cgcctcggga agagaacccg gaggccgaat cccgtgcggg ttccagttgg
    13681 cctgggtccc gccgctcaac cgtaccatgg gaggaagggc cacccccatg gcgaaagcgg
    13741 ccaggagtcc ccggcgggaa aggtccggca cggtgatctc tcccgacacc gacggccggg
    13801 cggcgtaggt catcaggtcc cgaccgcacc ggtcgaagca gttcatgcac aggacgcagt
    13861 catgagttcg aatggtgtcc gagggattgc aggcaccctc gcagtgagtt tcgcataagc
    13921 cgcaccggtt gcagtccccg ctcttctttg cgattttcca aagggcgaag tggctcaaaa
    13981 accccatgag ggcccccagg gggcagagga atcggcagta gaaccggggg gttttcaggt
    14041 tcagcaaaag agcccccagg aaaatcaccc cgatgaccca agccccgtaa tagaaccgct
    14101 ggtcggcatg gatccgctgg gaggtcgaat ccaggagagg gaggatggcg aggttcaccg
    14161 aacggtagag cagggggatg gggtccaaca ggcccgtctg gagagaaccg tcgatccatc
    14221 ggtccacggc gaagaagaag gccagacagg tccaaaaccc cagggctccc agcatcagtc
    14281 cggcggcttt ctttcgagga ccgaggaacc gaaagaggac aaggaccgaa agaaccaggg
    14341 tgcccacggc caggagagcc gggaggatcg aatggctgaa aacggtgcga atcccccatg
    14401 cagcgaggga gccggccccc gccgaaagga gaaaaaacaa gaggtagtac ttgacccgct
    14461 gtgctcctcg gtatgcattt tcggccgcgg ccgaggaagg atcccttctc cgtttgccca
    14521 catagccgag aaactggtgg agggttccga aggggcagac ccaaccgcag aaaaaccttc
    14581 ccagtacgag ggtcagaagg acggtggcca gggcccagag gagggtcgag tacagggtcc
    14641 gggtggtgag cacggtcccc agggcaagga gcggatcgag ctccatgaac cagttgacgg
    14701 gccagccgcg aagctgccgc cattgggtgc cgtaggagga aaccacgcag aaccaaagga
    14761 acaggaccag gaaaaagaac tggctgatgc gccttgcagt cacgattcgc atagtcggct
    14821 tttgtcaccc ggaaaggttg gtgcgcaacg gttgaaggga ctcgaagtcc acggttccgg
    14881 cccccgccgc ttccgccttg agtagaaagg ggagatcccg gggagacctt cccagcagag
    14941 tggctccgaa ggcgtccacg gccaccggat ccgtgcccgc aatcagggtg tgggtccttt
    15001 tcaggtcgga cagggatccc cccgtgggcc cgttggtcat catggtagtg gtcccgtcca
    15061 ggacgacgag ggtgggcctg atcagcaggg cgagttcctt gatcaactcg tggatattct
    15121 ggtgaaagac gtttctgcgt cctcccagga acccgtacca gttcttgagg accatggacg
    15181 ccccgcttcg gtggtggtcc ttgacgggag tgatgccgat gagtttgttc accccccgga
    15241 ggggttcgta gagtaggggc cagttgcgaa tgagggaccc tcccggcagg gtggccgagg
    15301 aaaagagctt ctcccgggga aggatcagtt gggctccggc cgagcgcacc gcttcgccaa
    15361 tgcccgtcag ggcaaagcag ctcgaagggt cgttgatggg gttgtcggtg acggcgacat
    15421 acgaggctcc cgcctggagg cagaggcggg cggtctccct caccaggtcc gggtgcgtgg
    15481 tggctcccaa caccgggggc atggcgaagg cggcgttcac cttgatgagc actcggtctc
    15541 ccttctttat gaacgcctcg atcccgccca gggcggtgac cgccgtctga agagcgcgaa
    15601 tgcggtcctc tccgtagacg atgctgatcc tcctgcccgc ttcgggcatg gtataattcg
    15661 gcagggtcag ggattcttcg ggcgggtttc cctgggtcgg tccgggcccc tcggggtcat
    15721 acaggccgta gcccagaaga cccgtcccca agaccacggc cccggtcccg gccaggcgcc
    15781 ggagaaattc cctgcggtct tccccgggaa gatctgcaga ttccatcagg gggcctcccc
    15841 agctattctt tcgtagaggg atcgagccac ccgggtagcc gaggcaaagg cctccgcctg
    15901 gtggactccg gcaagtgccc tgccggaact aaaaaccatc tccaaggtgt ccaggattgt
    15961 aatgcagtgc gcacccggca gcccgtctgc acactccacg gccttccccc cgttctccag
    16021 caggaaacgg acgtaaccgt ccgccaattg gcgggcttct tccgggtctc ggcgcaggga
    16081 gagaaatgcg gtgatcctcc cttcaggcaa ctcgtactcg gcggtaaagg tccggttcat
    16141 gaactcgaaa ccgaaaacgt tttccgccag gaggacccag ctgccttcca cccggtgttc
    16201 ggaagggaac agggcgagct cggggagggt ctcctcccgg cccggttctt gccgcaccag
    16261 tccttccgcc agcgcggcca tgacggcggg agattcctcg gccgccgaga cgatttcaac
    16321 gtagtggcga ccgtggacga aaaagagagc gttggctgtt tcgtaagcaa aaggggtgag
    16381 gggaacctcc cgggcgtcct cccgcctctg ggcggtaaag acggcaaagg cattcctcag
    16441 atctcccatg tcgtagaggt acagctccat ccaggattcg gaccgttctt tttccataaa
    16501 ccgctgggac cggagggagc gaaatccggc ggaaagatag agctccgcct ttccgttgat
    16561 cttctcggaa agggtttccg gggtgaaggt ctcgacgggg gacatgggca ccaggccgtc
    16621 gggcaacggc accggcaggt cccgtggtgc ggaagaaagg ggagactggg ggaggccttc
    16681 ctccgggctg gtcggggcga aaagggaggg gtcgaagcgc gcctgcttga cggccgttcc
    16741 cccggcgatc aaaaggagca ccaacaggac ggatgcgctg atgtagggtt ccctgccgtg
    16801 ctgcatcttc ttcaattccg ccgccctccg cgtggtagga ggaagatccg cccgtggcaa
    16861 ccggcctccg gcggccccga tgggttgtac cggcctatag taaccggccg ggacgcctcg
    16921 ggacaagcca ttcgtttcca tgttaacctt gttaactgag accaaacata cggcttgacc
    16981 gtttgggcgg cttctagtat agagtcatac cggcaaaggt aagtcccatc tcatgttatg
    17041 ggttcatgcg cgagtggttt tttcccacac tggtacgcgg ggaagttgga tcgaacgctg
    17101 cagctggagg tcgaaatgga aaacggtttg agctcgccca ttcaaaagat ttccactcta
    17161 acggaggacg ccaccaagag gaaaaaggaa gaggttttcg gctggtgggg gccttcgccc
    17221 gaagaagccc gcaagacgat ggttgggaaa tccaagggct tgcgggacaa gcggaccacg
    17281 atcaaggaag cggtcggtcg ttacatcaag gatggcgcca acattgccat cgggggcttt
    17341 gtgaataccc ggcctcccgt ggccattgtc cacgaaatca ttcggaaagg ggcgaaggac
    17401 ctgacccttt ccttccagtc caattccatt tgtcccgagc tcctggccgg ggccatgctc
    17461 ctgtacccgg accatttgtc cattcggcgt gcggaactgg cctggtgggg atatgaagtc
    17521 atcggcattg cccctctttt ccgatacttg tgcaacaacg gcctgatcga gctggatgat
    17581 tacacgaact acggcatgtc ggcccggttc aaggcggcgg ccatggggat cgaattcctg
    17641 cccgttcggg accacggcgg ttccgacatg gaactggtga atcgcgggat catggtccgg
    17701 agccccttta cgggatcgag ttcctatctg gttcccgcct gctatcccga cgtggggatc
    17761 ctcaacgtga cggcggccga catgtacggc aactgccgca ttttcggggc ccattgcacc
    17821 tgcccggaaa tcgccatggc ggcctgccac accatcgtga ccgccgagca gatcatcccc
    17881 aacgagaata ttcgaaccta tcccaacctg acggaaattc cctacacggc cgtagacgcg
    17941 gtgatcgaac agcgttttgc cagctatccg ggcgcgtgtt acggcttcta ctggtttgac
    18001 atggaacaca tcctcatgtt ccggagcgct tcggaagaat tccgcaagac cggaaagagg
    18061 gatgccctga agaaatatta cgacgagttc attttcgggt gtgaaaccca cgacgatttc
    18121 ctgagcaaac tcccctacaa aactctcaag aagatcaagg acctggacgg aggacagccg
    18181 atcatcctct cttagcgcga cggctcacgt gacgacggaa cggaaggaga aagggatcat
    18241 gcctgaatac acaccttttg aaatgattgc ctatacgggc tcgcgggtcc tcgaagacga
    18301 aaccatcgtg tttgtgggga cgggtttgcc catcattgct tccattcatg cccagctgac
    18361 ccacgcgccg ggcctgggca tcatctttga agcgggacct ctcaccccca tcctcgagat
    18421 gggaatgccc ctgtccgtcg gggacacgcg ggcctgcagg aaagcgtgtt atctgaaagg
    18481 tttgagttcg gtgttcgagc tcacccagcg ggggcattcc gactacgcat tcatcggcgg
    18541 cgcccagatc gacatgtacg gaaacgtcaa ctcgaccttc gaaggcggga cctacacccg
    18601 ggcccagacc cggtttcccg gaagcggcgg ggcaggcgcc atggcggcca actgcgagaa
    18661 atccatcatc atcatggccc tcgagcccag aagatttgtg ccccagctcg atttcgtgac
    18721 cagcatcggc ttcggggacg gatctccgga ctaccggaag aaatccgggg tcatgggttc
    18781 cggtccctac cgggtgatca ccaatgaagc cctgttcgga ttcgacgaag aaaccaaacg
    18841 catggtgctt ctcgaggcag ccaagggaag gacccccgag gacattcaga aaaaggtcag
    18901 cttcgatctt ctggtgtctc cggacctcaa ggaaatggcg gagccatccg aagaggacct
    18961 tcgtctcctg cgggaggaat gcgacccgga cggctatttt ctggccagaa aactgaaata
    19021 actcccgccc cgatcaggcc catccattcc tcttcccttc gtcttttcga gaagggagga
    19081 ggaatcgaac cccccctttc cccttgcccg gattgacaac aatttatgtc tcatggatcc
    19141 tggagaagat gccgatcaag aatcaaacgg gcgggtgggg ccccggggtg acaaacctcc
    19201 cgggcggccc cggaaaccag gctcgtttct ctccgagtgc ggggccttct ctgcacagtc
    19261 gtatttcgtt ggtcgttgct cggaagaggg ggatcgctcc cgaaaccccg ggatccgaat
    19321 gggcggaggg agggattctt ttttcctaac tcctttggac cgcgatacga gtatgggcat
    19381 cctcaactta gcggaattga agccggacat ggttttggcc agcgacctga aagccaggaa
    19441 cgggaggttt ctgctggcca aagggactcg gctcacgacc cagcacctga gggtcctcaa
    19501 gatctggggg gtggtggagg ccgatattgc gggagcggag tccagtgaag agggtttgga
    19561 ggcggcgccc gaaatcgatc cggcggtttg gaagagggcg gaaacgatct gccgggaaag
    19621 gttcatcctg accgatctcg atcatccggt gaaccaaaag ctccttcgcc tgagcattct
    19681 gaggaaggcc catgagcttt cgggatggcg acctcacggg gtcctgcagg cggagccgtc
    19741 tctcccggat gaggcgaacg gggcggggac cgaagaagga ttgagtctcc tggatccttc
    19801 ccaacttctc aaagaggaag tgcgcctttc cacgcttccc gtcatcctgg ctcaaatcat
    19861 cgaaaccatt aacaaaccga acagctctcc caaggagatc gccaacgtcg tcagccgaga
    19921 cacgaccctg tcggcccgac ttctgaggat cgtgaacagc gcgttttacg gctttcccca
    19981 caagatcgac accctgtccc gggccgtcct gattgtggga accaagcaga tgagcgccct
    20041 ggccatgggg gtcaaggtca tcaatatctt caagagcatt ccctgtgacc tcatcgatat
    20101 gaagtctttt ctcgaacaca gcattgcctg tgggatcgtg gcccggatcc tggcgggcca
    20161 caggagcatc accaataccg aaaggctctt tgtgggaggc ctgctccacg acatcggccg
    20221 cctggtgctc tacaatcaca tgccccatga agcgcgaagc gcccttctgg cagccaaggc
    20281 ggaaaagcgg cttctcaacg acctggaaga cgaaatgatg ccgtggaatc acgggcggat
    20341 cggaggctcc ctcctgaaaa agtggaggct gcccgtatcc ctggagacga tcacccggta
    20401 tcatcacgcg ccgacggtcg ccaaggaccc gacggaaccg gccatcatcc acgtggcgga
    20461 catcatcgtg aactccctgg ggatcggctc cagcggagaa cgccttgtgc cgccgttgaa
    20521 tcaacgggcg tggggtctct tggagttgtc cgtcaacacc ctggtgctgg cggtccagca
    20581 gacggacagt caattccagg agctccggcg cttcttgctg tgattttcca attggacgaa
    20641 aaaggtgaga aagagggccg ctccaaaaca gactctgaag ttctaccagg aaagagtcaa
    20701 tcacctggag gagctcaatc gatttaccct ggatgccctg gaaatggcgg cttcggtggg
    20761 agacttccag cccaatatca cccggctgga ggatatctcc gtcattctcc gggagaccaa
    20821 ggcgaggatc ttgcgcctga tccagtttca ggccgtcgcc tttttcatgg tggagcccga
    20881 ctccaacgat ttcaccctcg cctgcgtgga accggaagaa tttcgatcct atgtccagga
    20941 gcaggtggac cacctcatcg agaacggtac cttttcctgg agcctcatgg aaaagcggcc
    21001 ggtgattctc accagcaaga accagcagaa acagatcatc cttcacgtca tggccaccag
    21061 ttcgaggatc cgcggaatgt ttgtgggggt catggaaaaa gacaaaatgg acattccgga
    21121 tatttccctc tacctgttgt ccattgtcct tctcaacagc tccaatgcca tcgaaacctt
    21181 tcagctctac aacatgatcc gcgagatcaa caaggacctg gaaaacgaaa aggagaaata
    21241 caagtacctc ttcgaggctg cgcccgatgg ggtcgaggtg ctgagctccg acggcgccgt
    21301 catcgactgc aacgagaccc agagggtcat gaccggttac ctgcatgaag aaatcgtggg
    21361 aagaaaggcg gaagactttt tttctccgac gagcaaggcc ttgttcaaaa ccgaccccgc
    21421 agggggccgg ggagactgct tcgagcggga agtggagctc gtatgcaaag acgggtcggt
    21481 cttgccggtc tggagaaagg aaaaggtcat cctggacggg aaccggaact tcaaggggtc
    21541 cgtggtctat aaccatgatc tttcggccat tcgcaggact caacaggaaa agctgaacct
    21601 cgaggcccaa ctgaggcagg cccagaagat ggaggccatc ggcaccctgg cgggaggaat
    21661 cgcccacgat ttcaacaaca tcttgggggc catcctgggc tataccgaaa tggttctcta
    21721 caagttgtcc caggaggatc ccctccggcc cgatctcctc caggtctttc aggcgggaca
    21781 ccgggccaag gaactcgtgc ggcagatcct gaccttcagc cgccagtcgg aacagaaccg
    21841 gaatcctttg gacatcgccc ctatcgtcaa ggaaaccctc aagcttctcc gggcttccat
    21901 tcccagtacc attgaaattc ggcaggaagt gggggaacga atcggcagaa tcatgggaga
    21961 tcccactcag gtccaccagg tcgtcatgaa cctgtgcacc aacgccgcct acgccatgcg
    22021 ggaagcgggc gggctcctgg aagtggggct cgaacgggtg gcgtttcccc aacgcgcgtc
    22081 cgtgccccac cccaggttgg aaccgggggt ctatgtgcgg ttgaccgttc gcgactccgg
    22141 ggtcggaatg gagcccgcca cggtagagcg cattttcgag ccgtatttca ccaccaagcc
    22201 caagggagaa ggaaccggtc tggggctttc ggtcgtccac gggatcgtcc ggggctacgg
    22261 cggggtggtt accgtggaaa gcgagcccga ggaagggtcc acctttcggg tctatttccc
    22321 gcagatcgac ggggagagcg aggaactcca ggaggtgcag ccctcgattc ccggagggtc
    22381 cgaacgggtg atgttcgtgg atgacgagag atccctcacg attttgggga agaagatgtt
    22441 gggacatctg gggtacgagg tggtggcgtt gacgagcagc gtggaagctc tcgagtattt
    22501 tcgatcccac cgggattccg tggacctggt catcaccgac ctcaccatgc ctcacatgac
    22561 gggagatgtg ctggcccggg aaatgatgtc catccggccc ggcattccca tcatcttgtg
    22621 taccggtttc agtgactcca tgtcggaaga caaggccagg aggatgggtt tgcgggattt
    22681 catcaccaag cccctgatca tgaaggacct ggccagaacc atccgaaggg ccctggattc
    22741 cgtgtgatgc gaagttgcac tcaagccgaa atcatccatc caaaggatcc atcccttaga
    22801 tcgaaatctc cacgcaacat gtcggagaga ccttggacgc gattttgtgt gaaggcctgt
    22861 ccggaaagcc cccggttgcc tgtccgccgt tcgaagaccc cctgatacga agttacattc
    22921 acgccgaaac tgccgtcccg caggattcag ccatgtgctc aaaatcttca cgcagcatgt
    22981 cggatcgacc ttgggcgcaa cctggtatga atcgcccccc ggcggtccgt ccccgttctt
    23041 tttttacttg actttcacct tttatacacc tacattgcca ttgaaaccgc atcccacggc
    23101 cggtcgttcc cgcagacggg aacgaaaccc ttcgtcccgc aaaaagggga gaaaaggatg
    23161 gcgctcacac ccgcccagga aagagtctac cgtttcttga aacaatacct gcaggaacac
    23221 ggctgtgccc cttcctatga agaaattcgc cgacacctgg ggttccgttc cctcaacgcc
    23281 gtctccaagc atttgaagca gttggaacag cggggggttc tctccagccc gtgggggaac
    23341 cgaaaacggt ccctggagct gctacctttg cgcacggagg ccgcatccat tccttttctg
    23401 ggcatcgttg ccgccggcat tcccatcgaa gccgtggaag tccccgagat gatcgaggtg
    23461 cccgagagtt tcctgggggg cggcaacaat ttcgccctgc gggtgagggg ggattccatg
    23521 atcgaagaag gaatccggga cggggatatc ctgatcattt ccaagcagag tcatgcggaa
    23581 cggggccaga cggtggttgc cctcgtcaag ggagaagcca ccgtcaagaa attccatcct
    23641 tacggtccgg aggtggagct gatgcccgca aacagccaga tgaagcccat ccgtgttccg
    23701 gccggggacg tggaggtggt gggaatcgtc gtgggactgc tgcgccacta tcgttcccgc
    23761 tccgtccggt ccgtgcggga gtgagagccg ttcaaagaaa cccccatgga acgacggatt
    23821 gtatgcctgg agatccccga tttttacgcc gccttggagg agttgcgcag cccggagctt
    23881 cgcagggttc ccctggccct ggcggggccc ggaccccggg ccctcatcca gggggtcaac
    23941 cgtcttgccc gggaggaagg tcttcgggaa gggatggtcc tttcccgtgc ccaacggtct
    24001 tgtagaaagc ttcgagtcct tcctccggac cctcttttct acggggagca gcacgggaaa
    24061 aacctggatg agctgggccg ctactcccct ttggtggaag gaaccgtccc gggtcactat
    24121 tttgtggacc tgacgggcac ccgacgcctg tgggggccgt ccccggacgc ggccgtcatc
    24181 ctcgaacgtc ggctggagat gcagcgggga ttgaaggcac gggtcgggct cgcctccagt
    24241 aagctggtga gtcgggtggc ggccgggtgc gtcattgccg gggacctcgg ctgcgttttt
    24301 cccggcgggg aggtttcttt cctcgatccc ctgccggtgg cggctctccc cggggtgggg
    24361 gtgcagacga cttcccgcct ggccggcttc aatattcgac ggatcggcca gctggcagcc
    24421 ctgtccgttc gagacctcac ggcggtcttt ggaaccatgg gcgttcgcct ctctcagctt
    24481 gcccggggcg tggatccaac ccccgttctt ccctttcggc gaatccccaa gctggagttt
    24541 tcccggagaa tggatcggga tgaaatcgaa cgggagcgat tggaagcggt gcttttccgg
    24601 ctggtggaag atgccggatg ggccctgcgc acccggaatc gccatcccgg caaagtggcc
    24661 ctggaggttc gctatgccga cggaacgacg gcccggaggc agcataccct tgggcctcct
    24721 gcccggcagg tggatcggca gtggttccga atccttcggc cgaccctttc ccgtctcttg
    24781 gagcggcggg tggccgttcg ccggatgagg ctcgagctct gggaattggt catgcccctt
    24841 cggcagatgg agcttttccc gtgggaggag gtccccgtcg gggagagtcc gaggttgcag
    24901 gaagccctgg accgggttcg ccagcgtttt ggtcggcagg ccgttttttg gggcctgggg
    24961 aggaccgggt gatggggccc tttgtgcacc tccatgtcca ttcccattat tccttgatgc
    25021 ggggggtcag ttccgtggaa gctttgtgcc gggccggggc ggaggcgggg ttttccttcc
    25081 tggccctcac ggacaccaac ggattctacg ggctcgtcca cttcctggat gcggctcgac
    25141 gtcggcgcct tcgtcccctc gtgggagtat gcctcgaggc cccgggcgaa tccgccgttt
    25201 tcctggccaa aaccccccgg ggctatgagc tgctctgcga actggtttcc cgccgtcacc
    25261 tcgaggcggg gttttccctg gcgtcccacg ttccggaagc tccccgggac ctcgccctgc
    25321 tcagtgcgga cccccgcctg ctccgggtcc tgagccccag ggtccagtgt tgggtcgagg
    25381 tcctcccggg tccctgcggc cgcaaagccc tcgagatctc ccggtccctg gggattcctc
    25441 ccgtggcctc caacgccgtt catttcgccc ggcccgaaga ccattccctt caccgcctgg
    25501 tgcgggccat tcacttgaac cggaccttga gcaccctccc ggtcgaggag gtggtccaac
    25561 cggaccggtg gttgaagtcc gccggggaaa tggcccggca ttttccccac tgtcccgagg
    25621 ccctggccaa cacgcaaagg ctggcccggc aatgccacac ggcctgggga gactttcccg
    25681 cggtttttcc ccattaccgg gaccgggagg cggaccattt cgccatcctg gtccaaaagt
    25741 gccgccgggg aatcgcccgg cgctacggag aaagcaaccc cgccgtcgag gaacgcctcg
    25801 aggaggagtt gggcctcctt cggtccaagg gatacgtgga ttacttcctg gtggtggagg
    25861 acatcgtgcg gcgtcgtccc atccattgcg gtcgggggag cgctgcggcc agcctggtga
    25921 gctacctcct ggggatcacc cacgtggacc ccatccgtca tgggctcctt ttcgggcgct
    25981 ttctgaatcc tcatcgccgg gatcaccccg acatcgacgt ggattttccc tgggatgagc
    26041 gggacgggct ctgggaagaa ctggtccggt tctacggacc ccaacggctg gctgcggttt
    26101 ccaatcacgt ggggtttgga gcccgggcgg cgttgcgcga ggtggccaag gtctacggag
    26161 tccctgcggc cgagatcaag aacatgaccc ggcgaatgag ctcttggagt cggcccgggg
    26221 aaatgcggga tcgggtggac acccatccca agttccgggg acttgtcccg gagcctccct
    26281 ggccggaaat catgcagctg gccgcccgtc tcgagtccgt tccccggcac ctttcggtcc
    26341 actgcggcgg catcgttctg gcccccgacc gcgtttcccg gtatgtgccc gtgcagcgga
    26401 gcgccaaggg ggttcgggtg atccagtggg aaaaagacca ggcggaaagg gccgggctgg
    26461 tcaagatcga tcttctgggg aatcgctccc tggcggtgat ccgggacgcc ctcgagtcca
    26521 tccgggaaaa tacgggaaaa gagctcgatt atgccgggct caaccccatc gaagaccctg
    26581 ctacccggga cctccttgct cggggcgcca ctatgggggt cttttacgtg gaatctcccg
    26641 ccatgcgcca gttgcagcag aagaccggtc ggggagactt cgaacatcta acccttcatt
    26701 cctccatcat ccggcctgcg gccaatcggt acatccggga atacatccgg cggctgcacg
    26761 gggctcccta tgaacccctg cacccgaggc tcgaggagct cctggcggaa aactacggga
    26821 ttcttgcgta ccaagaggac gtggtccagg ttgcgagaat cctggcgggc ttcgactggg
    26881 acctggcgga cggcctgcgc aaggtgctca gcaaaaagag ccccgagcag atttccgcct
    26941 accggaagcg tttctacgaa ggatgcgccc gggagaacat cggcagggac gtggtggatg
    27001 ccgtgtggga tatgatcatc tcttttgcgg gatattcctt ctgcaagccc cattcggcct
    27061 cctacgccct ggtcagcttc aaatcggcct acctcaaggc ccactatccc gcagaattca
    27121 tggccggggt cttgagcaac gggggaggct actacagcac ccgggcctac atttccgaag
    27181 cccggcgcat gggattgacc gtcctgggtc ccgatgtaaa ccacagccgg tggacttacc
    27241 gggggaaggg gcgggtcgtt cgcatggggc tgcagcagct aaagggtgtg cggcgggaag
    27301 ccctggaaaa aatcctggac gagcgggaaa ggaacggacc ctttggttcc ctggaggatt
    27361 tccttcgccg aacggacccg gcccctgccg aaggggccgt cctggtcggg agcggcgcgt
    27421 tggacgccct ggcccgggga accctccccc ctttcgagaa ggccttcggt gcccacctcg
    27481 atcccgtttc cccggtcggg tccggggtcc gggaaacccg ggaaaccccg ggttcgggct
    27541 ttcagggagg cggcgggccg cggttttcgg gcaccgttcc cactcgtcct caaatcctgt
    27601 ggatggttga agcgacgttg aggggccgtt tttccggggg ggccggcggg acgatcggga
    27661 aaaatctctc gattccccgg cggtccttca cgggaggggt ccctccgctg ccggattaca
    27721 ccccggagca gcgatgggag caggaaaaag aaaccctggg gttcgtcctt tccgtccatc
    27781 ccctggccct gttcgagtcg gccctgaaaa gactgccgtt tcccgtcgtg cctgccgcgg
    27841 atctcgatcg gcacgtgggg cgggacgtgt gggtcttggg ctggcccatc acccgcaagg
    27901 aagtactgac ccgggatggg gaatccatgg aattcgtttc cctcgaagac caaacggcca
    27961 tctatgaaac ggttttcttt cccgacgctt ttcggcgctt ctgccgggac ctggattatg
    28021 atcgggctta tgtcctctac ggccgggtgg agagcgaatt cggtgctgta agcctcaccg
    28081 tccggaagct tcgccgactc tccctccccg acccccacgg gtctacttct cgaccttctg
    28141 aacatcggcc gccgaggtcc ccgtcaggtt cgtccgctga aacccgctgt ccgtgggcaa
    28201 ggccatgttg aaccggtaga ggtcgtcgat ctcgactttc acccgatcca gccgacagtc
    28261 caggacgtgg ccccctcgcc cggcatcctc ggtcaggaaa tgaaaatgat atccgggaac
    28321 gttcactccc ctcaggtatt cgggaaaccg gaagccgatg agggtcccgc gaacgttttg
    28381 cagatcgaag gacggctggg tcttcaccac ctccaccagg cgcgggtagg ggggggtctg
    28441 ccggggaacg ctgcgggtcc ggaccatggc aaaggatccc gtaatgcgaa aggcatagca
    28501 gaagttccgg gtgggcaagg tccggtccag gcgctcttcc agttctttgt aggaaagagg
    28561 ctcttccagg gagaaggccc ggtccgggtc aaagaaggtc atgatggcga agggggtgcg
    28621 cgaggaatcc tccaccgggt aggccgtgcc gtcggatttt acctggaaga accgggagtg
    28681 gagggcgatc atctcgccgt ccagggcgtt gaaggtgccc aggccggtgt cgccgtattt
    28741 tctcagttct ccgaaggtgg tttccccgtc atagacccct tccatgaggg cgttgatggt
    28801 cgaggcttgg aagacggtgt cctttggggg ttggggatga gcgcaggcgg acaagagcgt
    28861 cgtggccagg aggcagagta cgcataggga tcgaaaacgg ttcatgggac ttccttctct
    28921 tgttggccat cttcgggctt cgggtcgtgg ggggcagcgg ccaggtcccc cgcaaaacct
    28981 tcgggttgga acgtatcgtc accgggatcc aggccctccg gaactctggt gcctcgagac
    29041 ctctctcccg tccggctgaa ggttcgaggt cttccgacac ggcggtgggg aagggtccac
    29101 ccgagaaggg tccgggggcc ttgcggccct ttccggccgg agcaagccta gcacaaaatg
    29161 ttgacagttt tgagcaaaac tgttagggat gatccataat taggatggat cgttctttca
    29221 agcatctggc ggattggaac acaggaccga tttcggattt gacgggggtg ccgcgaaggc
    29281 gcccctttgc ttttaccgtt tcgatgggcg gcggcgcggc aaggaaacga ggggagcgcg
    29341 gggcgcccgc gcattcagcg ccgtcgcacg gaagggatgt cgtctttggc tgcagggacc
    29401 cgccgacggg ctcttgtccc ggcggatcgg ggattccggt tggagaagcg gggatcagcg
    29461 atgaaagaaa acagctcacg ggtggtaatg gaagccgatg agattcaacg agtgctggaa
    29521 cggttggcac ggggaatcat ggagaaatcc caggattccc aaaaactggc actggtggga
    29581 atccatacgg gcggagtgca cctggccagg aggattcaat ccgtcatccg ggagaaattc
    29641 gctttgtccg ttccggtggg gtcgttggac atcaatctct accgggacga
//
LOCUS       DAOOVC010000040        29495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1071_length_29495_cov_5.791135, whole genome shotgun sequence.
ACCESSION   DAOOVC010000040 DAOOVC010000000
VERSION     DAOOVC010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 29495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29495
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1071_length_29495_cov_5.791135"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLM79_07340"
     CDS             complement(<1..1183)
                     /locus_tag="PLM79_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86161.1"
                     /translation="MGEAERFDLVVIGAGPGGYVAAVRGTQLGMKVCCVDKDAKPGGV
                     CLNVGCIPSKALLDSSEYLHLAREHFREHGVLLSEVKLDLRAMMARKERVVRELTDNV
                     RTLLESNGVHLLRGTARLVGATRVEVTPGAPATGPVLLDSRFILLATGSEPVALPSAP
                     WDGIRILTSTEALSLDSVPERLGVIGAGYIGLELGSLWQRLGSTVTILEAMSRIAPPA
                     DAQVGRALERALRKQGLVFRLGTKLIEARPAEEEVKVSIESDGGREDLAFDRLLVAVG
                     RRPLTRGLGLEDVGVAVDSRTGHVRVDAQYRTSVPGIWAVGDLIAGPMLAHKASAEGI
                     AAVECMAGLPGEVDYDCIPSAIYTSPEVAGVGLTEEQAKERSIPYCVGTYPFSGAGRA
                     RE"
     gene            complement(1185..2582)
                     /gene="odhB"
                     /locus_tag="PLM79_07345"
     CDS             complement(1185..2582)
                     /gene="odhB"
                     /locus_tag="PLM79_07345"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOV86162.1"
                     /translation="MIVQVRIPEVGESVREALLVQWYRKDGDRVSKDEILFVIETDKV
                     TIEVVAEATGLLRILIREGETVPVGTVVGTIETQGAAESGEMAARQEVREEEPGLRRG
                     PSVDAIPPRQETSRSPAQSPLPSSQLGERSAPETLISPSARRLADENRVDVASISGTG
                     PQGRITRGDILLHMESSPDFVPTSKGPAGAIPPAGVLLPKTRATGESAEGSPGPRDVP
                     ARGKAAEDAPGTPPDERVTRTPMSPIRRRIAERLLQARQTTAMLTTFNEIDMSRVQAL
                     RARFKEPFHRKYGVSLGIMSFFIKAAVAALQEFLFFFTQKTAYDIVTPHFHHIGIAVG
                     ADRGLVVPVIRHADRLSFSQLELAIADFVQRTRENRLDLSDLEGGTFTISNGGIYGSL
                     LSTPILNFPQSGILGMHKIEDRPVVVDAQIVVRPMMYVALSYDHRIIDGREAVTFLLR
                     IKESIEDPERIMMEI"
     gene            complement(2649..5360)
                     /locus_tag="PLM79_07350"
     CDS             complement(2649..5360)
                     /locus_tag="PLM79_07350"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOV86163.1"
                     /translation="MNLPLAWNADFIQSRYERWKSDPHSVPRDWQMFFEGFSLAGTLE
                     LGGAPLCDAEHVLRQARVEELVHRYRDLGHLLACLDPLVSCPTSHPLLDPDRMGLGDE
                     DLERIFFTPHLPGMEQAPLKEILGMLRETYCRSVGVEYMHLQDPDERRWLQDRMEPTR
                     NRSVLDGESRRWIWEKLCRASLFEQFLHTRYLGQKRFSAEGAEVVVPALADLLDHSAA
                     SGVEEVVLGMAHRGRLNVQVNVLGKPYEAVFCEFEDHYDPDSLVGSGDVKYHHGYMAH
                     VSTRSGRKIRALLADNPSHLESVDPVIEGIARARQDLLGKDGIRRVLPVLIHGDAAFA
                     GQGVVSETLNLSQLEGYLTGGTIHLVINNQIGFTTLPEHARSTRYSTDIAKMLMVPIF
                     HVHGEDPEAAVHVIRLAYDYRFRFHKDVVIDIVCFRRYGHNEGDEPYYTQPTMYERIK
                     DRPPLFRLYGRKLMDEGVLTREEAEESGNRIHRELEDAYRSSRDKTCAAPEMEFYEAW
                     REYHGDLARLPDETGVDSDTLRELARQVHQLPPDLSVHPRLERILRRRAESVSAGEGL
                     DWATGEMLAFASLLDQGISIRLSGQDSRRGTFSQRHGVLVDTRTGACHTPLDRVARRG
                     ARLSVYDSMLSENAVLGFEYGYSIADPRPLVLWEAQFGDFANNAQVIIDQYLSSGELK
                     WGRLSGLVLLLPHAYEGQGPEHSSARLERFLQLCAQDNLQVVYPTTPAQYFHLLRRQV
                     LGRVRKPLVVLTPKSLLRHPRAVSSLGELSGGGFRPVLGPGAPTQNPRRVLLCTGKVY
                     YDLVERLDSRVGSQGIAILRLEQLYPFPESGLRRALAGWETAAEWIWVQEEPQNMGAW
                     SYLRPLLEELLPTPVRYAGRPPSASPVTGYHGIYEKEQKRLLEEALG"
     gene            complement(5370..5732)
                     /locus_tag="PLM79_07355"
     CDS             complement(5370..5732)
                     /locus_tag="PLM79_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HOV86164.1"
                     /translation="MTKLLFVISKGFEKAGGAVRAMQFAGIAAEKNHPVEVFLIDDAI
                     HWAQIGMAEGIRSTTGEHMKDFLEKLSEKRCPIHVCKACADKRLIGPDDLIPGAVIST
                     GGQLVEMMADPDTKVFTF"
     gene            6137..7303
                     /gene="sucC"
                     /locus_tag="PLM79_07360"
     CDS             6137..7303
                     /gene="sucC"
                     /locus_tag="PLM79_07360"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOV86165.1"
                     /translation="MKIHEYQAKELFKKYGVPIPRGKAAFSVEEAKAIGAELNTFPVV
                     VKAQIHAGGRGKGGGVKLAKSAAEVEQYAGSILGMTLVTHQTGPEGKVVKKLLVEEGL
                     PIEKELYLSVLPDRGTAKIVFMASEAGGMDIEEVAAKTPEKIIKVYIDPLMGFKGFHA
                     AELAFGLNLKPELIKQFNAMVAALYKLCVDYDCSLVEINPLVITSDNRVIALDGKINF
                     DDNALYRHKDIQEYRDLDEEDPFEVEASKYDLNYIKMPGGNIGNMVNGAGLAMATMDL
                     IQQAGASPANFLDVGGGASAEQVENGFRIILADPAVKGVLINIFGGILRCDRLANGVV
                     EAAKKVGINVPVVIRMEGTNVEQGREILEKSGLNLITAKDVSDAAQKIAAIAKR"
     gene            7318..8196
                     /gene="sucD"
                     /locus_tag="PLM79_07365"
     CDS             7318..8196
                     /gene="sucD"
                     /locus_tag="PLM79_07365"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOV86166.1"
                     /translation="MSIWVDKNTRLLVQGITGREGQFHTLACKNYGTNVVAGVTPGKG
                     GQNVEGIPVFNTVAKAVRETGANAAMVFVPPAFCADAIIESIEAEVPVIVAITEGIPV
                     MDMMKVKNYLKSSKSRLIGPNCPGIITPGQCKIGIQPGHIHTPGPVGIVSRSGTLTYE
                     AVHQIGMMGIGQSTVIGIGGDPVNGTNFIDCLSAFQEDPETKAIIMVGEIGGSAEEDA
                     ADFVKKNVTKPVIGFIAGLTAPPGRRMGHAGAIISGSSGTAQGKIKAMREAGITVVEN
                     LGEMGRIAKEAFKEVI"
     gene            complement(8180..8563)
                     /locus_tag="PLM79_07370"
     CDS             complement(8180..8563)
                     /locus_tag="PLM79_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOV86167.1"
                     /translation="MKQISKEDQLVIRSKIWIEDQEGKVVFGSGRLKILDAVERHGSI
                     LAASKEMNMSYRAVWGKIQATEARLGQPLLYRKAGGARGGGSQLTPLGKTLVERFRQL
                     QKLTETTANGLFQDLFVEGLVISPP"
     gene            8952..10223
                     /locus_tag="PLM79_07375"
     CDS             8952..10223
                     /locus_tag="PLM79_07375"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOV86168.1"
                     /translation="MSTFLRVKSTREVLEIVRKFQPLGAETVPLGRAWGRVLAEPVHS
                     REPVPHFPRATMDGYAVRARDSFGASENLPALLEVSGHVCMGEGYAGEVEPGKAVSIP
                     TGGILPAGADAVVMIEFTHPLDETTIEVFKPVAPGENVLGIGEDIAEGQEVFPRGRRL
                     RSQDVGVLAALGLPEIPVYRRPRVAVLSTGDEILPAFTPSLPPGKVRDINSFTLAAMV
                     EESSAVVGTCGILCDHLDSLVSACCDALDRHEVLILSGGSSVGSRDLTIEVLERLADA
                     QILVHGIAVRPGKPTILARVDDKLVWGLPGQPVSAVMIFQTFVRPSLERLQGLEGVDF
                     ADSGFPRPAVLNRRLPSVHGRTDLVPVILSREDSTWLATPVFGKSAMIHVLARSDGYV
                     LVPEHVEGLDQGTPVNVYPYSLRGGSVGQGG"
     gene            complement(10292..10900)
                     /locus_tag="PLM79_07380"
     CDS             complement(10292..10900)
                     /locus_tag="PLM79_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOV86169.1"
                     /translation="MSDSNDPRVVIPHGIQEEPRGPVWYPKLTGNELCWFALYVQVNH
                     EKEVTRRLEERSVDCYLPLVESWSKRRDRRKRIHTPLFPGYVFVHSVLDNYSHVHILK
                     TPGAVSLVRNSEGALPIPDFQIDNLKTLIESSAPLSPHAYLNRGDWVQVIRGPMAGCC
                     GILLRHNPKKGRLVVSVDIIQRSVSVELDVEDVEPVRSPVRP"
     gene            complement(11028..14015)
                     /locus_tag="PLM79_07385"
     CDS             complement(11028..14015)
                     /locus_tag="PLM79_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV86170.1"
                     /translation="MSEPTSQDQEPVIQRYLQALLHQERADRKVLHHAVLPAREAQHA
                     NPAIPLPETLGKILARQGITRLYSHQAAAIEHIRAGRNVVVATPTSSGKSLIYNLSVT
                     ERLLKEPGIRALYLFPIKALIRDQLANLEALLKTGREEDPAFSFTSAVYDGDIPPYQR
                     SKIRRHPPHILLSNPDMLHYALLPFHHKWETFWKSLRFVVIDEMHTYRGIFGSQVSQV
                     FRRLQRICRYYGSSPQFIFLSATIANPGQLAGQLVGDFHEFEIVRENGAPQAQRHFIF
                     LETGDLQATHASGVAARLVVQAARMGLKTIAFTQSRRLTELVHLSVMRMDPRLAKRVS
                     SYRAGFLPEERRSIEQKLAGGSLDAVISTSALELGIDIGGLDVCILVGYPGTVMTTWQ
                     RGGRVGRSGRESAIILLPQSDALDQYIAHHPREFLESPYEIAVTDPFNPEILKAHLPC
                     AAAEEPLERLEREFQGEAYQRALNLLTQGGRLHRTSDGNQWISGVHNPHRRVDIRSVG
                     ASFAILRRAESPGEKSIPLGKNEGIRALRECHPGAVYLHRGESYQVESLDLEKRVIQV
                     TQCNVPYFTRVKTDKETEILEILASKPSGNFLIRLGRLKVTEHILGYEKRRLFSQELL
                     GTSPLDLPPQEFETVGFWVEIEPSIAASVQKAQLHFMGGIHAVEHAMISIFPLFALCD
                     RNDIGGISCPQHPQVGKGAIFIYDGYPGGIGLAARGYEIILPLLEKTRELIASCGCQD
                     GCPACIHSPKCGSGNKPLDKGAALSVLRYLVGEWKLTEEIPQETGESPADRKARPHAE
                     PEERPPRMGFLDLETQRLADEVGGWGNKHLMGISVAVVYDTEFEDFRVYREEEVPALV
                     DHLEQLDLVVGFNILSFDYAVLQAYTSVDLNRLPTVDILKEIHAHLGFRVGLHHLAEK
                     TLGKAKGADGVQAVRWFRQGAWDSLIEYCMLDVALTRDLFQHALDKGYLVYQNREGQT
                     LRIPTPWASDRVRKAQRKTGDSR"
     gene            14225..14857
                     /locus_tag="PLM79_07390"
     CDS             14225..14857
                     /locus_tag="PLM79_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV86171.1"
                     /translation="MQHEDWIKKIHWLGHDCLRLDGSVTVYFDPFRISPGVGADLILI
                     THDHYDHCSPEDVEKIRKKETTIVTDAASARKLEGDVRVVKAGDRLQVKGVEIEVHPS
                     YNTNKTFHPRKAGMLAFVVTLDGVRYYHAGDTDLIPEMEGLTVDVAFLPVSGTYVMTA
                     EEAVQAARRIKPKVAIPMHYAAIVGTEQDALAFRDALRGEMEVVVLTRES"
     gene            14889..15818
                     /locus_tag="PLM79_07395"
     CDS             14889..15818
                     /locus_tag="PLM79_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86172.1"
                     /translation="MAAYVQACKDGSLAEKIRRGLRWLTKCTLCPRMCRVDRTAGETG
                     YCRTGRHAVVASHGPHFGEESPLVGRRGSGTIFFSHCNLFCTFCQNYDISHGGEGVPV
                     GAEGLADLMVHLQNRGCHNINFVTPSHVIVQILEALPIAVERGLEVPLVYNTGGYDRV
                     AALKLLDGIVDIYMPDFKFWDPGVASELCNAPDYPQRARDALREMHRQVGDLRTDGAG
                     IAYRGLLVRHLVLPQGLAGTGDVLGFLARELSTNTYVNVMDQYHPCGNAVGSPQMGRR
                     VTGKEFGEALRMAEEAGLTRLDQRHRHWLTIEL"
     gene            15842..16924
                     /locus_tag="PLM79_07400"
     CDS             15842..16924
                     /locus_tag="PLM79_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOV86173.1"
                     /translation="MARIDAFFKLMNEQKASDLHLVSGQQPVLRIRGELERVKYKVLD
                     DDDLRAMLYEITPEDKIKTFEETGDLDFAYEIPGLARYRANFFRQKNGIGAVFREIPS
                     EILSTEQLGLPAVVSRLAMLPRGLVLVTGPTGSGKSTTLAAVIDEANRKRKDHIITVE
                     DPIEFVHVSKSCLVNHREVGSHTMSFSTALRAALREDPDIILVGEMRDLETIRLAIEA
                     AATGHLVFSTLHTTSAAKTVDRVIEVFPTGEQAQIRSTLADGLRAVVSQTLFKRIDVK
                     GRMAVLEILIATHAVRAMIREAKTHQIPSALQTGKKYGMQTLDDGILAALKAGKIGPD
                     DAYAKCVDKEKFRPYLTEPPLDFTEV"
     gene            17145..18314
                     /locus_tag="PLM79_07405"
     CDS             17145..18314
                     /locus_tag="PLM79_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOV86174.1"
                     /translation="MRRKELDYVLTRILEENPRASDLNFTVGKPMQAEVDGRLKAVCP
                     DLIADKLTPFHTETIAMNLIAGDRRNIQNLLNQGSCDLSYHLPGKARFRVNVFSQRGT
                     YSIVMRQLPTKIASIEELRLPPVFKKVAEEKFGIVFVTGATGSGKSTTLAAILNEINL
                     QKAVHVVTLEDPVEFVHPQKKATFNQRELGLDFDAFASGLRAALRQAPKVILVGEMRD
                     RETVEIGLSAAETGHLVMTTLHTIDAGQTINRLVGMFELEEERLIRIRLADTLRWAIS
                     QRLLPREGGGRVAAFEIMGNNLRVRESIINGESEGKTFYEMMEQAQPFGWMTFDSCIV
                     RHYEQGYISEETAMAYASKKAVVKRGIDQIRSARGEKTTDIEGLRIDQEYARRIE"
     gene            18319..18984
                     /gene="thiE"
                     /locus_tag="PLM79_07410"
     CDS             18319..18984
                     /gene="thiE"
                     /locus_tag="PLM79_07410"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOV86175.1"
                     /translation="MSSSGLWPPDRSLYVVTDCRWLEGRSLEDVVAAALRGGAGVIQY
                     REKTASTRHMVETASKLCALCRRHGAVFLVNDRVDVALAADAHGVHLGQEDMPARAAR
                     RILGPGKILGVSIHDEEEILRAEEDGADYVSVSPVFATSTKPDHQSPLGVRGTCVLVR
                     RSRLPVAVIGGIDETNLEPLVRCGVRGICVVSAVMAARDPEGASRRLCLGIREVLKTL
                     PQS"
     gene            19020..20036
                     /gene="thiL"
                     /locus_tag="PLM79_07415"
     CDS             19020..20036
                     /gene="thiL"
                     /locus_tag="PLM79_07415"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOV86176.1"
                     /translation="MKITDIGEFGLISRIAGMLPRHPPGVLVGIGDDVAVLEVSPGGV
                     LLATCDIQVEDVHFRRAWITPYQLGRKVVAINVSDIAAMGGSPAWALVSLALPRDTRV
                     EFVDELYGGMQEEISRAGGAIVGGNVSTIHSRVVIDLFLMGRADPEKLIRREGARVGD
                     RILVTGTLGDSRAGLELIQRPDLKVDSAHARRVLDRHRTPSPRWREGQCLASSGRVHA
                     MVDVSDGVLSDVGHICRASAVGAEIRLSDLPVSDPCREVARAAGKDWEDWALTGGEDY
                     ELLFGVAEDQADEVARLVEEETGTPCRCIGSFIPRDEGIFVSAPGKALKKDLSRFRGW
                     DHFR"
     gene            20053..20922
                     /gene="thiD"
                     /locus_tag="PLM79_07420"
     CDS             20053..20922
                     /gene="thiD"
                     /locus_tag="PLM79_07420"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOV86177.1"
                     /translation="MKIPKALTIAGSDSGGGAGIQADLKTFAALGVYGMSAVTALTAQ
                     NTRGVEGILEVDAEFVSRQVHAVITDIGVDAVKTGMLANAAIISRVAEDLKALKVKCL
                     VVDPVMVAKSGDRLLRPEAVESLIHQLLPLATVVTPNLQEAECLASMPVRGPEDMKEA
                     AKRILDFGPRTVVIKGGHLEGTPLDLFFDGLEVRRFTNPRYDTVHTHGTGCTYASAIT
                     AYLARGMGVADAVGRAKEYITGAIREGLPLGSGQGPVHHFHRFYSLVGWNWGTSRLKT
                     EKTWPGGREENHG"
     gene            20915..21556
                     /locus_tag="PLM79_07425"
     CDS             20915..21556
                     /locus_tag="PLM79_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86178.1"
                     /translation="MAEKRRMIPSDGLLLDARWEEGTTSDAVLLCHPHPLYGGSMDNN
                     VVEAMQTGFAGLGWGTLRFNFRGVGRSEGRHTQGEGETRDVLGMASHVRREANRVLHL
                     AGYSYGAWIGLKAVNQGLDPASLVLISPPVGLMDFESLSLPLRPVLVIAGDEDEFCPP
                     DSLRSWLDGAGGSREGVELEIFHGCDHFYWGIERRLADRIREFLQRCVLRTKV"
     gene            21705..22094
                     /locus_tag="PLM79_07430"
     CDS             21705..22094
                     /locus_tag="PLM79_07430"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOV86179.1"
                     /translation="MEDRFALQGESLVVDRQTGLVWQRGASGDRMVWKDGFRYVEDLN
                     GKQFAGFGDWRYPTKDELATLILPEEDRKTGLFVHPYFGTQRNCWTSTETGRHRACYV
                     DFYYGDVYVIEEAYANHFIRAVRSNPQ"
     gene            22122..22661
                     /locus_tag="PLM79_07435"
     CDS             22122..22661
                     /locus_tag="PLM79_07435"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOV86180.1"
                     /translation="MRAAGENNGGGNVPPLRVILIEPEIPQNTGNIARTCAATHTPLH
                     LVEPLGFLLTDRHLKRAGLDYWPHVRLKVHRDFDSLHQELRHGRWVCFSAGAPRDYFD
                     FRFRAGDCLLFGPETRGLPSRVLEAAGPNLLRIPMDRTRVRSLNLSTTVGVVLFEAIR
                     QLSFESAGFLSAFAPLSGP"
     gene            22686..22838
                     /locus_tag="PLM79_07440"
     CDS             22686..22838
                     /locus_tag="PLM79_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86181.1"
                     /translation="MNSGSSLRETGASSIKRPDSDGLLRRLYEVCPEIVQLGLFPVVL
                     GKGSRY"
     gene            23062..23517
                     /locus_tag="PLM79_07445"
     CDS             23062..23517
                     /locus_tag="PLM79_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86182.1"
                     /translation="MAEIEWKGATWRAAYGDLSVKELLTILKGFGPMEILEFERPGRV
                     RGQVSLSLSAKGRKQITLYYLEVLGERRRGRGREALEWLRGIFKGELYVEDPGIIHVK
                     NPNAESLLFWIAMYREGLIQAMESEFCILRPGMREKELTEIEKKLVEVR"
     gene            23814..24566
                     /locus_tag="PLM79_07450"
     CDS             23814..24566
                     /locus_tag="PLM79_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV86183.1"
                     /translation="MKIRGTKRAVFGEEEVVGALERFQDFLERNRMWSYGALVVLILC
                     VAGFWGFRSYAESRERRAAETYARISVTFPDDEQGDAGKWAALVPELEKFVSEHSDSP
                     VRINAWMDLAKACFNLRRYDKAAEAASRAVEESGPRSPLFGLARYQLALIYQEMGKVD
                     EALAQWTALKGDSIKGFGREIEWRLAQLHEKKGDYTRATEQYEAALKAPGSYPPAPLL
                     SEQLASLKTKTPTPSFPSPQTPAAEVADKPRS"
     gene            24863..26164
                     /gene="paaF"
                     /locus_tag="PLM79_07455"
     CDS             24863..26164
                     /gene="paaF"
                     /locus_tag="PLM79_07455"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV86184.1"
                     /translation="MIYDIEFETLPREALEAIQLRRLQSVVERVYATVPFYRKKFQES
                     GVRPGDIQSLADLRRLPFTTKQDLRDNYPFGMFAVPMDNVVRIHASSGTTGKPTVVGY
                     TARDIHTWAELMARSLAAAGGTRGDIIHNAYGYGLFTGGLGIHYGAERLGASVIPISG
                     GNTKRQILIMKDFGPTILTCTPSYALHLAEVAAEAGIDFSRLNFKAGIFGAEPWSEGM
                     REEIERKLHLKALDIYGLSEVMGPGVSMECIEAKNGLHVYEDHFIAEIINPDTGEVLP
                     HGETGELVFTTITKEAFPVIRYRTRDVTSLIPEPCVCGRSHIRMKRVSGRTDDMLIIR
                     GVNVFPSQIESVLMEIEEVEPHYQLVVDREGNLDTLTVMVEIGEEAFSDEVRKLQALE
                     KKISKNIKEYLGVSATVKLVEPKMIARSEGKAQRVIDKRKF"
     gene            26185..26616
                     /locus_tag="PLM79_07460"
     CDS             26185..26616
                     /locus_tag="PLM79_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOV86185.1"
                     /translation="MKVEQISVFLENKAGRLAEVTRILMESGVNIRALSLADTSDFGI
                     LRLIVNDNNKAKEALRARGFTVGKTDVVAVEVEDRPGGLHRILDVLYRANVNVEYMYA
                     FVQQSGSNAVIIFRFDNLEEAVKVLTEHGVPVIEPEKLYSI"
     gene            26942..28102
                     /locus_tag="PLM79_07465"
     CDS             26942..28102
                     /locus_tag="PLM79_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86186.1"
                     /translation="MIPRNSKIGKALILGGMVLLLLASSVGWCKEPYKIGGVFSITGP
                     NSFLGDPEKKSLEMAVEEINAKGGIDGHPLQVIIYDTEGDPTKAVLAVNKLISRDQVI
                     AIIGPSNTPPTLAVVPIAEKEKVPLISCAAGIKITDPVKPWVFKTAQTDALAVAAIYA
                     HMKKQNLKKIGILTVENAFGESGKEQLTGQAAAAGIQVVQGETFGAKDTDMTAQLTKI
                     RTAQPDAVVCWGTNPGPAVVSKNVKQMNLKMPLYQSHGVASPKFIELAGDAAEGILLP
                     TGKILVAKQLPDSDPQKAVLLRYIEDYEKRYQTPVSGFGGYAYDAMQILAKALRGTEG
                     DKAKIRDNLEKVTGHVGVSGTFNFSATEHNGLGPDAFVMVQIQGGAWKLLAP"
     gene            28118..28993
                     /locus_tag="PLM79_07470"
     CDS             28118..28993
                     /locus_tag="PLM79_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV86187.1"
                     /translation="MQEFLQYVFSGVTNGAVYAVIALGFSMLYRATGLINFAQGELVM
                     LGALCLISLWQGLGLSLVLSIVLSVGIVSGVGIALERLAMRQVRKPHPIVLVIITVGA
                     SIFFRGIAMILWGKDARSAGPFSSNVPLEIAGATLLPQSLWVLAIMLGIVAALHLFYR
                     KTLTGKAMEACAINKKAAWLLGVPSDRMVLLAFALSAGMGAVGGVLIAPITMSSYDMG
                     TLLGLKGFCAAMLGGLGSSWGSVLGGLLLGILEALGVGFFSSALKDAIAFMLLLFILY
                     VRPTGILGARAVHRF"
     gene            29013..>29495
                     /locus_tag="PLM79_07475"
     CDS             29013..>29495
                     /locus_tag="PLM79_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV86188.1"
                     /translation="MLIRNLLFAAAILLLGVGVKNPYHLELLTFIGIHTLLALGLNML
                     MGYAGQISLGHAAFYGLGAYATGILTVHWHWSPWISLPATLALAAAVALLVALPMLRL
                     SGFYLGMGTLGFGMIGFILFRGWSSVTGGDSGLVGIPSLAVGPFTFSSGVRYFYLVWG
                     A"
BASE COUNT         5821 a         8630 c         9345 g         5699 t
ORIGIN      
        1 cttctcgagc tcgccccgcc cccgagaagg gataggtgcc tacacaataa ggaatggagc
       61 gttccttggc ttgttcttcg gtgaggccca ccccggcgac ttccggggag gtgtagatgg
      121 ccgatgggat gcagtcgtaa tccacttccc ccggcagacc cgccatacat tccacggcgg
      181 cgatcccttc ggcggaggct ttgtgagcca acatgggccc ggcaatgagg tccccgacgg
      241 cccagatgcc gggaaccgag gttcggtatt gggcgtccac ccgcacatgg ccggtccggg
      301 aatccacagc gacccccacg tcttccagcc ccagtccccg agtgaggggg cggcgtccca
      361 cggcgaccag gagacgatcg aaagccaggt cttctctccc cccgtccgat tcgatggaga
      421 ccttcacctc ctcctccgcc ggtcgagcct cgatcagttt cgttcccagg cggaatacca
      481 atccctgctt tctcaatgcc cgctccaggg cccggcccac ctgggcatcc gcgggcggtg
      541 caatacggga catggcttcg aggatcgtta ctgtcgaccc gaggcgctgc cacagggaac
      601 ccagctcgag gccgatgtac ccggcgccga tgaccccgag cctttccggg acggaatcga
      661 gagacagggc ttcggtggag gtaaggatcc ggatcccgtc ccagggggcc gagggaagag
      721 ccaccggctc gcttcccgtc gccagcagaa tgaaccggga atccaggagg acgggtcccg
      781 tggccggggc cccgggggtc acctccaccc gggttgctcc cacaagccgc gccgttcccc
      841 ggagaagatg aacgccgttg ctttcaagaa gcgttcgcac gttgtccgtg agctcccgca
      901 cgactctttc ttttctggcc atcatggcgc gaagatccag cttcacctcg gacagcagga
      961 ctccatgttc ccggaaatgc tcgcgggcca ggtgaaggta ttcgctcgag tcgagcaggg
     1021 ccttgctggg aatgcagccg acgttcaggc agactcctcc cggctttgca tccttgtcca
     1081 cacagcacac cttcattccc aactgagtcc cacggacggc cgccacgtac ccgcccggac
     1141 cggccccgat cacgaccaga tcgaatcttt cggcctctcc catgtcaaat ctccatcatg
     1201 atgcgttcgg ggtcttcgat ggattccttg atgcgcagca ggaatgtgac ggcttcccga
     1261 ccgtcgatga tgcggtgatc gtagctcagg gccacgtaca tcatggggcg cacgacgatc
     1321 tgagcgtcga cgaccacggg acggtcctcg atcttgtgca tccccaggat tccgctttgg
     1381 gggaaattca ggatgggagt gctgagaagg gaaccgtaga ttccaccgtt gctgatcgtg
     1441 aaagtcccac cctcgaggtc ggagagatcg agccggttct cccgggtcct ttggacgaag
     1501 tccgcgatgg cgagctccaa ttgggaaaag ctgagccggt cggcgtgacg gatgaccggc
     1561 accacgagac cgcggtccgc gcccacggca atgccgatgt gatgaaaatg gggggtcacg
     1621 atatcgtatg ccgtcttctg cgtgaaaaaa aagaggaatt cctggagggc cgcgacggcg
     1681 gccttgatga aaaaggacat gatccccagg gacaccccgt acttgcgatg gaacggttcc
     1741 ttgaagcggg ccctcagggc ctgaaccctg ctcatgtcga tctcgttgaa ggtcgtgagc
     1801 atggcggtgg tctgccgtgc ctggagcaac cgttcggcga tgcgtcggcg aatggggctc
     1861 atgggcgtgc gcgtcaccct ctcgtcgggc ggggttcccg gggcgtcctc cgcagccttg
     1921 ccccgtgcgg gaacatcccg gggacccggg gaaccttccg ccgattctcc cgtcgccctg
     1981 gtttttggaa gaaggacgcc ggccggggga atcgccccgg cgggcccttt tgaggtcggc
     2041 acgaaatccg gggaggattc catgtgaaga agaatgtccc cacgagtgat tcgaccttgc
     2101 ggtccggttc ccgaaatgga ggcgacgtcc acccggtttt cgtccgccag gcggcgtgcg
     2161 gacggggaga tgagcgtttc cggggcgctt ctttcaccga gttgggagga aggcaaaggg
     2221 gattgcgccg gggaccggga tgtttcctgc cggggcggaa tcgcgtcgac ggaaggtccc
     2281 cggcgcaacc cgggttcttc ctcccgcacc tcttggcgag ccgccatttc cccggattcc
     2341 gcagcccctt gggtctcgat ggtccccacc acggtaccca ccggcacggt ctctccctcc
     2401 cggatcagaa tcctcaggag tcccgtcgct tccgcaacga cctcgatggt gaccttgtcg
     2461 gtctcgataa cgaacagaat ctcgtccttg gacacccggt ctccgtcttt cctgtaccac
     2521 tgcaccaaga gggcttccct cacggactcc ccgacctcgg ggattcttac ctggacgatc
     2581 attgcgggct cctaagctcc tcaaagctct ctctttgcat gaccgaggct tccgccacgg
     2641 gcaacgaatc aacccaaggc ctcctcgaga agcctcttct gttccttttc gtagatgccg
     2701 tggtaccccg tcacgggact tgccgaaggg ggacgacccg cgtaacggac gggggtcggc
     2761 agcagttcct ccaggagagg gcgaaggtag ctccatgcgc ccatgttctg cggttcctcc
     2821 tgcacccaga tccattcagc cgcggtttcc cagcctgcca gggccctcct caggccgctc
     2881 tcgggaaaag gatacaactg ctcgagcctc aagatcgcga tcccctggga cccgacacgg
     2941 gaatccagcc tctcgaccaa gtcgtagtac acttttccgg tgcacaaaag gaccctgcgg
     3001 gggttctgtg tcggggcccc gggacccagt accggacgga accccccgcc gctcagttcc
     3061 ccgaggctcg aaacggcccg gggatggcgc aagagactct tgggagtcaa gacgaccaag
     3121 ggctttcgga cccttcccag gacctgtcga cggagcagat gaaaatactg tgcgggggtt
     3181 gtggggtaga cgacctgcag gttgtcctgg gcgcacagct ggaggaatcg ctcgagcctg
     3241 gcgctggaat gttccggccc ctggccctcg taggcgtggg gcaaaaggag caccagcccg
     3301 ctcagccgtc cccacttgag ctccccgctc gagagatact ggtcgatgat cacctgggcg
     3361 ttgttggcaa aatctccgaa ctgggcttcc cacaaaacca ggggccgggg atcggcgatg
     3421 gaatacccgt actcgaaacc cagcacggcg ttttccgaga gcatgctgtc gtagacggac
     3481 aggcgagcgc ccctccgggc aacacgatca aggggagtgt ggcaggctcc cgtgcgggta
     3541 tccaccagga ccccgtgccg ttggctgaag gtccctcgac ggctgtcctg gccgctcaaa
     3601 cggatggaaa tcccctggtc gaggaggctg gcgaacgcca gcatttcccc ggtggcccaa
     3661 tcgagcccct cccccgccga aacgctctcg gcccgtctgc gcaaaatgcg ctccaaccgt
     3721 ggatggacgg agaggtccgg gggcagctgg tgcacctgtc gggccagctc cctcaaggtg
     3781 tcggaatcga ctccggtctc atccggcaat cgggccaagt ccccgtggta ttcccgccag
     3841 gcctcgtaga attccatttc cggcgccgcg caggtcttgt cccggctcga tcggtacgca
     3901 tcctccagtt cccggtggat tcggttcccg gactcctccg cctcctcccg ggtcaaaacc
     3961 ccctcgtcca tgagcttccg gccgtagagc cgaaacaggg gagggcgatc cttgatgcgc
     4021 tcgtacatgg tcggttgggt gtaatagggt tcgtccccct cgttgtgccc gtaacggcga
     4081 aaacacacga tgtcgatgac gacgtctttg tggaaccgaa accggtagtc gtaggcgagg
     4141 cggatcacgt gaacggccgc ttcgggatcc tccccgtgga cgtgaaaaat ggggaccatc
     4201 agcatcttgg cgatgtccgt cgaataccgg gtggatcgcg cgtgctcggg cagggtcgtg
     4261 aaaccgatct ggttgttgat gaccaagtgg atcgtgcccc ccgtgaggta cccctccagc
     4321 tgggacaggt tgagggtttc gctcaccacc ccctgcccgg caaacgccgc atccccgtga
     4381 atgagcacgg gaagcacccg gcgaattccg tccttgccca ggaggtcttg gcgggctcgg
     4441 gcaatgccct cgattaccgg gtcgacggat tccaggtggc tgggattgtc ggccaacagg
     4501 gcacggatct tcctgccgct gcgggtggag acgtgggcca tgtatccgtg atggtacttg
     4561 acgtccccgc ttcccaccag ggagtccggg tcataatggt cctcaaactc gcagaagacc
     4621 gcctcgtaag gcttgcccag gacgtttacc tggacgttga gacgtccccg gtgggccatc
     4681 cccagtacga cctcctcgac acccgaagcc gcactgtggt ccaagagatc ggcgagggcg
     4741 ggcaccacca cttccgcccc ttcggcggag aaacgcttct ggcccaggta ccgggtatgg
     4801 aggaactgtt cgaacagact cgcccggcag agcttttccc agatccaccg cctggattct
     4861 ccatcgagga ccgaccgatt tcgagtgggt tccatccggt cctggagcca tcgacgctcg
     4921 tcggggtcct ggaggtgcat gtactcgacc ccgacggagc ggcaataggt ttcccggagc
     4981 atccccagga tctccttcaa cggcgcctgc tccatgcccg gaagatgcgg ggtaaagaag
     5041 atcctttcga ggtcctcgtc gcccaggccc atacggtccg ggtccaaaag aggatggctg
     5101 gtagggcatg agacgagagg gtccaggcag gcaagcagat gtcccaggtc ccggtaacgg
     5161 tgaaccagtt cctccacacg agcctgccgg agcacatgct cggcatcgca gaggggagcc
     5221 cctcccaact cgagggtccc cgccagggaa aagccttcaa aaaacatctg ccagtcccgg
     5281 gggacggagt ggggatcgga cttccaccgt tcatatcggg attggatgaa atcggcattc
     5341 catgccaggg ggaggttcat ctcccgtccc taaaacgtga agactttggt gtcggggtcc
     5401 gccatcatct ccaccagttg gccgccggtg gaaatgacgg ctccgggaat gagatcgtcg
     5461 ggcccaatga gtcgtttgtc cgcacaggct ttgcacacgt ggatggggca gcgtttctcc
     5521 gagagctttt ccaggaaatc cttcatgtgc tcccccgtgg tggaacgaat gccctcggcc
     5581 atgccgatct gtgcccagtg aatggcgtcg tcgatgagga agacctccac gggatggttc
     5641 ttttccgcgg caattcccgc aaactgcatg gccctgacgg ccccgccggc tttttcaaaa
     5701 cccttggaaa tcacgaacaa taatttcgtc atgctctatt cccccccaat cggtttcgtt
     5761 gacggattcg gaaacccact tcccccgcgc ttccgggttt tccgggcacc ctgcggttcg
     5821 ttccaaaggg tctgcggcga agggcgggat cgtgggcgca acggtgccgg aaggcaagcg
     5881 ggaacggaga agttccattc gcagcaagat ccgggtgtga atcaagctta agtcaagcaa
     5941 cccgccgagt caatcgggag cgatttcgga cccccgggat gttaaagaat ttacaaacaa
     6001 tgtttgacac ggaaagatct ttcatctaga aagaatgaaa ttaagggagg agcggcagaa
     6061 ttttttggat cgggctctcg ttgtaaaacg tggtctgcag agaatgcgaa ctgtttctgc
     6121 tagagcgagg tgaaagatga aaatccatga gtaccaggcg aaagagttat tcaagaagta
     6181 cggagtccca attcccaggg gaaaagcggc cttcagtgtt gaagaggcca aggccatagg
     6241 agccgagttg aatacgtttc cggtggtggt caaggcccag attcacgccg gggggcgtgg
     6301 aaaaggaggc ggggtcaagc tggccaagag tgctgccgag gtcgagcagt atgccgggag
     6361 catcctgggc atgaccctgg tgacccacca gaccggtccg gaaggaaagg tggtcaaaaa
     6421 gctcctggtg gaagagggac tgcccattga aaaagagctc tatctcagtg tgcttcccga
     6481 ccgagggacc gccaagatcg ttttcatggc gagcgaagcc gggggtatgg acatcgagga
     6541 agtagccgcc aagactcccg agaaaatcat caaagtctat atcgatcccc tcatgggctt
     6601 caaaggcttc catgccgccg aactggcttt cggcctgaac ctgaagcccg agctgatcaa
     6661 gcagttcaat gccatggtcg cggccctcta caaactctgc gtggactacg actgctccct
     6721 ggtggaaatc aatcccctgg tcatcacttc cgataatcgg gtcattgccc tggacggcaa
     6781 gatcaatttt gacgacaacg ccttgtatcg tcacaaagac atccaggaat accgcgactt
     6841 ggacgaagag gaccccttcg aggtcgaggc atccaagtac gatctcaatt acatcaagat
     6901 gccgggaggc aatatcggca acatggtcaa cggcgccggt cttgccatgg cgaccatgga
     6961 cctcattcag caggccggtg cctctcccgc caatttcctg gatgtcggcg gcggagccag
     7021 cgccgagcag gtggaaaacg gcttccgaat catcctggcc gatcctgcgg tcaagggcgt
     7081 gctcatcaat attttcgggg gaattctccg atgtgaccgg ttggccaacg gggtggtgga
     7141 agcggccaaa aaagtgggca tcaacgtgcc cgtggtcatc cgcatggaag ggaccaacgt
     7201 agaacaaggc cgggaaatcc tcgagaaatc cgggctcaat ttgataacgg caaaagacgt
     7261 ttcagatgcc gcccaaaaaa ttgctgccat tgccaagaga tagaaggggg aaaaatcatg
     7321 agcatttggg tggataagaa tacaaggttg ttggttcagg gaatcacggg tcgggaaggt
     7381 cagttccata ccttagcgtg caagaactac ggcaccaacg tggtggcggg ggttacgccg
     7441 ggcaaaggcg gccagaacgt cgaaggaatt ccggtgttca acacggtggc aaaggcggtc
     7501 agggaaacgg gcgccaatgc ggccatggtc tttgtgcctc ccgcattctg tgccgatgcc
     7561 atcatcgaat ccatcgaggc ggaagtcccc gtgatcgtag ccatcaccga aggcattccc
     7621 gtcatggaca tgatgaaggt taagaactac ctgaaatctt ccaagagccg cctcatcggc
     7681 cccaactgcc ccggcatcat caccccgggc cagtgcaaga tcggcattca gccgggccac
     7741 attcacaccc cgggccccgt gggcatcgtg tcccgctcgg ggaccctcac ctacgaagcc
     7801 gtgcaccaga tcgggatgat gggcatcggc caaagcacgg tcattggaat cggtggggac
     7861 ccggtgaacg gaaccaattt cattgactgc ctcagcgcct tccaggagga ccccgagacc
     7921 aaggccatca tcatggtggg tgaaatcggc gggtctgcgg aagaagacgc ggccgatttc
     7981 gtcaagaaga acgtgaccaa gccggtcatc ggcttcatcg ccggtttgac ggcgcctccg
     8041 ggccgccgca tgggccatgc gggcgccatc atcagcggat cgagcggtac ggctcagggc
     8101 aagatcaagg ccatgagaga ggccggaatt acggtggtgg aaaacttggg cgagatgggt
     8161 cggatcgcca aggaagcctt caaggaggtg atataaccag tccttctacg aataagtcct
     8221 ggaacaaacc gttggctgtg gtttcggtaa gcttttggag ctgccgaaac cgttccacga
     8281 gcgtcttgcc caggggggtg agctgagagc cgcctccccg ggcgcctccg gccttccggt
     8341 acagaagcgg ctgccccagt cgcgcctcgg ttgcctggat ctttccccat acggcccgat
     8401 aactcatgtt catctccttc gacgccgcca ggatggaccc gtgccgttcg acggcatcca
     8461 ggatcttgag acgccccgat ccgaaaacca ccttgccttc ctggtcctcg atccagatct
     8521 tggatcgaat gaccagctgg tcttccttcg aaatctgttt cacggaaatc ctctccctcc
     8581 caggcttgtt tcgggacccc gcctcaacgg ggaaaacgat gccgggtaag gtcccggaac
     8641 gcctgcggcc gccaaccgcg gcatgggacc gcctttgctt cgccggaacc gggtcgaatc
     8701 cgttcttggg accatggacc caaggaccgt aagtacgacc tcaattcgta tactataccg
     8761 ggattgaggg ccccttggca accaatccct gctgttttgc cgggagctgt ggtacaagaa
     8821 agggggaagc ccgcccaggg cccacaggca ggcaaagttc gttccggtag gcaatcccgt
     8881 gccgacatgg gtgtccgtgg cgcggggcta ccgggcagat cgttcaacag gggaatagac
     8941 gggaaaatac catgtctacg tttctaaggg tcaaatcgac ccgggaagtt cttgaaatcg
     9001 tccggaagtt ccaacccctc ggagccgaga cggtcccgct cgggagggcc tggggccggg
     9061 ttcttgccga acccgttcac tcccgggaac cggtgccgca ctttccccgg gcaaccatgg
     9121 acggctacgc cgttcgggcc cgggacagtt ttggggcctc ggaaaacctg ccggccctcc
     9181 tcgaggtgtc cggacacgtg tgcatgggag agggctacgc cggagaggtc gagccgggaa
     9241 aggccgtttc catccccacg gggggaatcc taccggccgg ggccgatgcc gtcgtgatga
     9301 tcgaattcac ccaccccctg gacgaaacca ccatcgaggt cttcaaaccc gttgccccgg
     9361 gggagaacgt gctgggaatc ggggaagaca ttgccgaggg gcaggaagtg tttccccggg
     9421 gccggcgatt gaggtcccag gacgtgggag tccttgcggc tctcggactg cccgagatcc
     9481 cggtctaccg gcgtccccga gtggcggttc tctccacggg agacgagatc cttcccgctt
     9541 tcaccccgag tcttcccccg ggaaaggtgc gggacatcaa cagcttcacc ctggccgcca
     9601 tggtcgagga atcctcggca gtggtaggca cctgcggcat tctttgcgat catctggatt
     9661 ccctggtctc tgcctgttgc gacgccttgg accgccacga ggtcctgatc ctgtcgggag
     9721 gaagctcggt gggaagccga gatctcacca tcgaagtcct cgaacgactg gccgacgctc
     9781 agatcctggt ccacggcatt gccgttcgcc cgggaaaacc caccatcttg gcccgcgtcg
     9841 acgacaagct ggtgtgggga ctccccgggc aacccgtttc agccgtcatg atctttcaaa
     9901 cctttgtgag accctctctg gaaaggcttc agggattgga aggggtggat tttgccgact
     9961 cgggattccc gagaccggcg gttctgaacc ggcgactgcc ctcggttcat ggaagaacgg
    10021 atcttgtgcc ggtgatcctg tctcgggagg actccacctg gctggccaca ccggtgttcg
    10081 gaaagtccgc catgatccac gtactggccc ggtccgacgg gtatgtactg gtgcccgagc
    10141 acgtggaggg tctggaccag gggacgccgg tgaacgtcta tccctattcg ttacggggag
    10201 ggtcggttgg gcaggggggg tagggtccag gggctgggag gctgggaagc gaaggggtga
    10261 aagcgggggg ccggggtgcg cccccctcgg gctacggtct taccggggaa cgaacgggct
    10321 ccacgtcttc cacgtccagt tccacgctca cggaccgctg gatgatgtcc acgctcacca
    10381 ccaaccgtcc tttcttggga ttgtggcgca ggagaattcc acagcagccg gccatgggtc
    10441 cgcgaatgac ctgaacccag tcgccccggt tcaggtaggc gtgcgggctc aggggcgccg
    10501 aggactcaat cagggtcttt agattgtcga tctggaaatc cgggatgggg agagcgcctt
    10561 cggaattccg aacgaggctc acggctccgg gggttttcag aatatgcacg tgggagtagt
    10621 tgtcgaggac ggaatgaacg aacacgtagc ctggaaacag cggcgtgtga atccgtttcc
    10681 ggcggtccct cctcttgctc caggactcca ccaggggaag ataacagtcg acggatcgtt
    10741 cctcgagacg cctggtcact tccttttcgt ggttcacctg gacgtagagg gcaaaccagc
    10801 atagttcatt ccccgtcagt tttggatacc agaccgggcc gcggggctct tcttgaatcc
    10861 cgtgggggat cacgaccctg ggatcgttgg aatcactcac gccttgtccg cctccagagt
    10921 tccctttctt ggggccgccc tgccttccct ttgaagacgc cggacctgct ccgggaatat
    10981 cgccggagga tgcgcggggt ccgataatcc gttgaggggc gaacccgtca acgagaatcc
    11041 ccggtttttc tttgggcttt ccggacccgg tccgatgccc agggggtggg gattctcagg
    11101 gtttgacctt cccgattttg atacaccagg tagcccttgt ccagggcatg ttgaaaaaga
    11161 tcccgggtca gggccacgtc gagcatgcaa tactcgatga gggaatccca tgctccttga
    11221 cgaaaccacc ggaccgcctg gactccgtcg gctcctttgg cctttcccag cgttttttcg
    11281 gccaggtgat gcaaccccac ccgaaacccc aggtgggcgt ggatctcttt gaggatgtcc
    11341 acggtgggga gccggtttag gtccacggac gtgtacgctt ggagcaccgc atagtcgaag
    11401 gacaggatat tgaatcccac caccaggtcc agttgttcga gatgatccac cagggccgga
    11461 acctcctcct cccggtaaac ccggaagtcc tcgaactcgg tatcgtagac cacggccacg
    11521 gaaatcccca tgaggtgctt gttgccccaa cctcccactt cgtcggccaa gcgctgagtt
    11581 tcgaggtcga ggaaccccat gcgcggggga cgctcttccg gttccgcatg ggggcgggcc
    11641 ttcctgtctg cgggggactc gccggtttcc tgagggatct cttcggtcag tttccactct
    11701 cccaccaggt agcgcagcac ggagagggcg gcgcccttgt ccaggggttt attgcccgag
    11761 ccgcacttgg gagagtggat acaagcgggg cagccgtcct ggcaaccgca ggaggcgatc
    11821 agttcccggg ttttctcgag gagcggcagg atgatttcgt agccccgggc cgccaacccg
    11881 attcctcccg ggtagccgtc gtagatgaag atggcccctt tgcccacctg gggatgttgc
    11941 gggcaggaaa tgcccccgat gtcgtttcgg tcacaaaggg cgaaaagggg gaagatgctg
    12001 atcatggcgt gctcgacggc gtggattccc cccatgaaat ggagctgcgc cttctgaacg
    12061 ctcgcggcaa tggaaggctc gatctcgacc caaaagccga cggtttcgaa ttcctggggc
    12121 gggagatcca ggggagaggt ccccaagagc tcctgggaaa agagccgtct tttctcgtag
    12181 cccaggatgt gctccgtcac cttgaggcgc cccaaccgaa tgagaaaatt tccggacggc
    12241 ttggacgcca agatttcgag gatctcggtt tccttgtccg tcttcacccg ggtaaaatag
    12301 ggcacgttgc attgggtcac ctggatcacc cgcttttcca ggtccaggct ttccacctgg
    12361 taggactctc cccggtggag atagacggct ccgggatggc actcgcgaag ggctcgtatt
    12421 ccctcgtttt tccccagggg gatgctcttt tccccggggg attccgcccg gcgcagaatg
    12481 gcaaaggaag ccccgacgct gcggatgtcc acccggcggt ggggattgtg aacccccgag
    12541 atccactggt ttccgtcgga cgtccggtgc aatcgccccc cctgggtcag taggttcagg
    12601 gctctctgat acgcctcgcc ctggaattcc ctttccagcc tttcgagggg ttcttcggcg
    12661 gcggcacagg gaaggtgggc cttgaggatt tcgggattga aggggtcggt taccgcgatc
    12721 tcgtaggggc tttcgagaaa ctcccgggga tggtgggcga tgtactgatc gagggcgtcc
    12781 gactggggca gcaggatgat ggccgattcc cggccgctcc ttcccacccg tcctccccgc
    12841 tgccaggtgg tcatcaccgt gcccgggtag cccaccagga tgcagacgtc caggccgccg
    12901 atgtcgatcc ccagttcgag ggcgctggtg gaaatgacgg cgtccaagga tccccccgcc
    12961 agtttctgtt cgatggagcg ccgttcctca ggaaggaacc cggcccgata agagctcacg
    13021 cgcttggcga ggcgggggtc catgcgcatg acgctcaggt ggaccaactc cgtgagtcgc
    13081 cgggactggg tgaaggcaat ggtcttcaac cccatccgag cggcctggac caccagtcga
    13141 gccgcaacgc ccgaggcgtg ggtcgcctgg aggtctccgg tctcaaggaa aatgaagtgg
    13201 cgttgggcct ggggcgctcc gttttcccgg acgatctcga attcgtggaa atcccccacg
    13261 agctggccgg ccagttgccc cggattggca atggtggcgg agaggaaaat gaactggggg
    13321 gaacttccgt aataccgaca aattctttga agcctgcgga acacctggct cacctggctc
    13381 ccgaaaatcc cgcgataggt atgcatctcg tcgatgacga cgaaccggag gctcttccag
    13441 aacgtctccc acttgtggtg aaaggggagg agagcgtaat ggagcatatc ggggttgctc
    13501 agcaggatat ggggaggatg ccggcggatc ttcgatcgct ggtagggggg aatgtccccg
    13561 tcatagacgg cggaggtgaa cgagaaggca gggtcctcct ccctgcccgt cttgagaagg
    13621 gcttccagat tggccagctg atctcgaatg agtgctttga tgggaaaaag gtaaagagcc
    13681 cggattcccg gttccttcag cagacgttcg gtgacgctca aattgtagat gagggacttg
    13741 ccgctggagg tgggtgtggc caccacgacg tttcgtcccg cccggatatg ctcgatggcc
    13801 gcggcctgat gggagtaaag tctcgtgatg ccctggcgcg ccaggatttt gcccagggtc
    13861 tcgggcaaag ggatcgccgg gttggcgtgt tgggcttccc gggccggaag aacggcgtgg
    13921 tgaaggacct ttcggtccgc tctttcctgg tggagcaaag cctgcaggta gcgctgaatc
    13981 acgggttcct gatcctggga agtgggttca ctcatcacag ggttccacgg aaaggccccc
    14041 cggatcccgg tccgtcacag taacccaaaa gacaaccgtt gggcaagctc ggacacggga
    14101 ccccgaggtc cggaaaaggc ccccatgggc tctggatggg cttccgtggt ttcgcttcta
    14161 tcgttgggcg ggttatgata gtatcgtcgg gcgaaattct ccggtccccc acaaggagga
    14221 gcccatgcaa cacgaggatt ggatcaagaa gattcactgg ctgggacacg attgcctgcg
    14281 cctggacgga agcgtgacgg tctacttcga ccccttccgg atttccccgg gggtgggcgc
    14341 ggacctcatc ctcatcaccc acgaccacta cgatcactgt tctcccgaag acgtggagaa
    14401 gatccggaag aaagagacga ccatcgtcac cgacgccgcc tcggctcgaa agctcgaggg
    14461 cgacgttcgg gtcgtcaagg ccggggatcg attacaggtc aagggggtcg agattgaagt
    14521 ccatccttcc tacaacacca acaagacgtt ccatccccgg aaggcgggaa tgctggcctt
    14581 tgtggtgacc ctggacgggg tccgctatta ccatgcggga gacacggacc tgattccgga
    14641 aatggagggc ctgacggtgg atgtggcctt tctccccgtg tccggcacct acgtcatgac
    14701 ggccgaggaa gcggtgcagg cggcccggcg gatcaagccc aaggtcgcca ttcccatgca
    14761 ctatgcggcc atcgtcggca cggaacagga tgccctggca tttcgggatg cccttcgggg
    14821 ggagatggaa gtggtggtcc tgacccggga atcctgaaaa gtctccccgg gaaaagagga
    14881 aagcgccgat ggcggcatac gtgcaggctt gcaaagacgg ttccttggcg gagaagattc
    14941 ggcgcggcct gcggtggttg accaaatgca ccctgtgccc tcgtatgtgt cgagtcgatc
    15001 gaacggccgg ggagacgggc tattgccgga cggggcgtca cgccgtggtg gcgagccatg
    15061 gtccccattt cggagaagaa tctcccctgg tgggcaggag agggtccggc accatctttt
    15121 tttcccactg caatctgttt tgcaccttct gccaaaacta cgacatcagc catgggggcg
    15181 agggggttcc cgtgggagcc gagggtttgg ccgaccttat ggtccacctg caaaaccggg
    15241 gatgccacaa catcaatttc gtcactccct cccacgtgat cgtgcagatt ctcgaagcct
    15301 tgcccatcgc cgtggaacgg ggactcgagg tgcccctggt gtacaacacg ggcggatatg
    15361 accgggtggc ggccctcaag ctcctggacg ggatcgtgga catttacatg ccggacttca
    15421 agttctggga cccgggggtg gcctccgagc tttgcaacgc tcccgactac ccgcaacggg
    15481 ctcgggacgc cctgcgtgaa atgcatcgtc aggtgggaga tcttcgaacg gacggggccg
    15541 gaatcgccta ccgggggctc ctggttcgcc acctggtgct gccccaaggt cttgcaggca
    15601 ccggggacgt cctggggttt ctggcccgcg aactttccac caatacgtac gtgaacgtca
    15661 tggaccagta tcatccgtgc ggcaacgccg tagggtcgcc gcagatggga agaagagtca
    15721 cggggaagga gttcggggag gcgttgcgga tggccgagga ggcggggctg acccgcctgg
    15781 atcagcggca caggcattgg ctgaccatcg agttgtagac gctctgaggg agggggtttg
    15841 tatggccagg attgacgcat ttttcaaact gatgaacgaa cagaaggcat ccgaccttca
    15901 tctggtatcc ggacagcaac cggtgctgcg gatccgagga gagctcgagc gcgtcaagta
    15961 caaggtcctc gatgacgacg accttcgggc catgctgtac gaaatcaccc ccgaggacaa
    16021 gatcaagacc ttcgaggaaa cgggcgacct ggactttgcc tacgagatcc cgggactcgc
    16081 ccgttaccgg gccaacttct tccgccagaa gaacggcatc ggggccgtct ttcgggagat
    16141 tcccagcgag atcctctcca ccgaacagct ggggttgccc gccgtggtga gtcgcctggc
    16201 catgttgccc cggggcctgg tgctggttac gggaccgacg ggaagcggga agtccaccac
    16261 cctggcggcg gtcatcgacg aggcgaatcg gaagcgcaag gaccacatca ttaccgtcga
    16321 ggaccccatc gagtttgtcc acgtgagtaa gagctgcctg gtgaaccacc gggaggtggg
    16381 gtctcatacg atgagctttt ccacggcgct gcgggcggct ttgcgcgagg accccgacat
    16441 catcctggtg ggggaaatgc gggacctgga aaccatccgc ctggccattg aagcggcggc
    16501 caccgggcac ctggtcttct cgacccttca caccaccagt gccgctaaga ccgtggatcg
    16561 tgtgatcgag gtgtttccca cgggagagca ggcccagatc cgctcgacct tggccgacgg
    16621 acttcgggcc gtggtttccc agaccctttt caagcgcatc gacgtcaagg gtcgaatggc
    16681 cgtcctcgag attctcatcg ccacccacgc cgtgagggcc atgattcggg aggcgaagac
    16741 ccaccagatc ccgtcagccc tgcagacggg gaagaagtac gggatgcaga ccctggacga
    16801 cggaatcctg gctgcactca aagccgggaa gatcggtccc gacgatgcct acgccaagtg
    16861 cgtggacaag gaaaaattcc gcccttacct gacggagccg ccgctggact ttacggaggt
    16921 gtagagcccg gccgcgcaca aggattccgt ccccctcgca caccttcccg ggccgtaaga
    16981 catgggagag gaagccgtcc cgcctccttg ggcgggagtg aacggagaag cgtaaagggt
    17041 ctgttgggaa aagaatcccc ggcgtgcggg tccgaccccc cggtggttct ccccggggat
    17101 tccggccgcg gatccgcaaa ggaattttca ttgggggtga aatcatgaga agaaaagagt
    17161 tggactacgt tctaacccga atcctggagg aaaacccgag ggcttccgac ctcaatttca
    17221 cggtgggcaa acccatgcag gcggaggtcg acggccggct caaggcggtc tgccccgatc
    17281 tcatcgcgga caagctcacc cccttccaca cggaaaccat cgccatgaac ctcatcgccg
    17341 gggaccgcag aaacatccag aacctgctga accagggttc ctgcgacctt tcctaccatc
    17401 tgcccggaaa ggcacgattc cgcgtcaatg tattctccca gcggggcacg tactccatcg
    17461 tcatgcggca gttgcctacc aagatcgctt ccatagagga actccggctg ccccccgttt
    17521 tcaaaaaggt cgccgaggaa aagttcggaa tcgttttcgt gaccggcgct acgggaagcg
    17581 gcaagtccac gaccctggcc gccatcctca acgagatcaa tcttcagaag gccgtgcatg
    17641 tggtgaccct ggaagacccc gtggaattcg tacaccctca gaaaaaggcc accttcaacc
    17701 agagggagct ggggctggat ttcgatgcct ttgccagcgg gctccgggcg gcccttcgcc
    17761 aggcccccaa agtcatcctg gtgggagaga tgcgcgatcg ggagacggtt gaaatcggtc
    17821 tgagcgccgc cgaaacgggg cacctggtta tgacgaccct tcataccatt gatgcgggcc
    17881 aaaccattaa ccggctggtg ggaatgttcg agctcgagga agaacgactc atccgcattc
    17941 gtctggcgga cactctccgc tgggccattt ctcagcggct gctccccagg gaggggggag
    18001 gccgggtggc ggctttcgaa atcatgggaa acaacctccg ggtgagggaa tccatcatca
    18061 acggagaaag tgagggcaag accttctacg aaatgatgga acaggcccag ccgttcggat
    18121 ggatgacttt cgacagctgc atcgttcgcc actacgaaca aggctacatc agtgaggaga
    18181 cggccatggc ctacgcttcc aagaaggccg tggtgaagcg gggcatcgac cagattcggt
    18241 cggcccgggg cgaaaagacc acggacatcg aagggctcag gatcgaccag gaatatgccc
    18301 ggagaatcga ataaggcaat gtcttcatcg gggttgtggc ccccggatcg atccctttac
    18361 gtggtgacgg attgccgttg gctggaaggc cggtccctcg aggacgtggt cgctgcggcc
    18421 cttcgagggg gtgcgggggt gatccagtac cgagaaaaga ccgcttccac ccggcacatg
    18481 gtggaaacgg cctcgaagct ttgcgccctg tgtcgacgac acggcgccgt ctttctggtg
    18541 aacgaccgcg tggatgtggc cctggcggcg gatgcccacg gagtccatct gggacaggaa
    18601 gacatgcccg cgagggcggc aaggcgaatc ctgggtccgg gcaaaatcct gggagtgagc
    18661 attcatgacg aagaagaaat ccttcgggcc gaagaagacg gagcggatta tgtttccgtc
    18721 tctcccgtct ttgccacttc caccaagccc gatcaccaaa gtcccctggg ggtccggggc
    18781 acctgtgtcc tggtccggcg aagccgactt cccgtggcgg tcatcggcgg aatcgacgaa
    18841 accaacctgg aaccgttggt tcggtgcggg gtccggggaa tctgcgtggt gtcggccgtc
    18901 atggccgcga gagaccccga gggggcatcg agaaggctct gcctcggaat tcgggaagtc
    18961 ctgaagaccc tcccccaatc atgaggtcgg cccggagggg tcccgggaag gatttgggat
    19021 tgaagatcac ggatattggc gagttcggct tgatatcccg gatcgccggg atgctgcccc
    19081 gccacccgcc gggcgtgctg gtgggcatcg gggatgacgt ggcggtcctc gaggtttccc
    19141 ccgggggggt ccttctcgcc acctgcgata tccaggtgga agacgtccat tttcgccggg
    19201 cctggatcac tccctaccag ttgggtcgaa aagtggtggc catcaacgtg agcgacattg
    19261 ccgccatggg aggcagcccc gcctgggcgc tggtgtccct ggccctgccc agggacaccc
    19321 gggtcgagtt cgtggacgag ttgtatgggg ggatgcagga agagatttcc agggccgggg
    19381 gggccatcgt ggggggcaat gtgagcacca tccattcccg ggtggtcatt gaccttttcc
    19441 tcatgggccg ggcggatccc gagaagctga tccggcgaga gggggcccgg gtgggagacc
    19501 ggattctggt tacggggacc ctgggtgatt cccgggcggg cctcgagttg attcaaaggc
    19561 ccgacctgaa agtcgattcg gcccatgccc ggagggtctt ggatcgccat cggaccccca
    19621 gtccccggtg gagggaggga caatgcctgg catcctcggg ccgggttcat gccatggtgg
    19681 acgtgagcga cggggttttg agcgacgtgg gccacatctg cagggccagc gccgtagggg
    19741 ccgaaattcg gctctcggat cttcccgtca gcgacccttg ccgggaggtg gcccgagcgg
    19801 cgggcaagga ctgggaggac tgggcactca ccggcgggga agactacgaa cttcttttcg
    19861 gggtggctga agatcaggcg gacgaggttg cacgattggt cgaggaagaa acgggaactc
    19921 catgccgctg catcggcagt ttcattcccc gggacgaggg gatcttcgtc tcggcgccgg
    19981 ggaaggcatt gaagaaggat ttatcccggt ttcggggatg ggaccatttt cgttgaaagg
    20041 gcgggttttc cggtgaagat acccaaagca ctgaccatag cagggtccga ctccgggggc
    20101 ggagccggga tccaggcgga cctcaaaacc tttgcggccc tgggcgtcta cggcatgagc
    20161 gccgtcaccg ccctcaccgc ccaaaacacc cggggagtgg aaggaatcct cgaggtggat
    20221 gccgagttcg tctcccggca agtccacgcg gtgatcacgg acatcggggt cgacgccgtg
    20281 aaaaccggga tgctggccaa cgcggccatc atttctcggg tggccgaaga tctgaaagcc
    20341 ctgaaggtga aatgcctggt ggtggacccg gtgatggtgg ccaagagcgg agaccgcctc
    20401 ttgcgacccg aagccgtgga aagcctcatc catcagctct tgcccctggc tacggtggtg
    20461 actcccaacc tccaggaggc cgaatgcctg gcatccatgc cggtacgggg cccggaagac
    20521 atgaaggagg cggccaaaag gatcctggac ttcggacctc gaaccgtggt gatcaagggc
    20581 ggccacctgg agggaactcc cctggacctt ttcttcgacg gcctcgaggt tcgccggttc
    20641 accaaccctc gatacgacac ggttcatacc cacgggaccg gatgcaccta tgcctccgcc
    20701 atcacggcct acctggcccg gggcatgggg gtggcggacg cggtcggccg agccaaggaa
    20761 tacatcacgg gcgccatccg ggagggcctc cccctgggaa gcgggcaggg ccccgttcat
    20821 cattttcatc gattctattc cctggtcgga tggaactggg ggacgtcccg tctgaaaacc
    20881 gagaagacgt ggccgggagg cagggaggaa aaccatggct gagaagcgac ggatgattcc
    20941 ttcggacggg cttctcctgg acgcgcgatg ggaagaggga acgacctcgg acgccgtcct
    21001 cctctgtcat cctcatcccc tttacggcgg aagcatggac aacaacgtgg tggaggcgat
    21061 gcagacgggc tttgcgggcc tgggctgggg caccctgcgg ttcaatttcc ggggcgtggg
    21121 tcgcagtgaa ggacgccaca ctcaggggga gggtgaaacc agggacgttt tgggaatggc
    21181 gtcgcacgtg cgccgggagg caaaccgagt gcttcacctg gccgggtact cctatggggc
    21241 ctggattggc ctcaaggccg tgaatcaagg gctcgatccc gcttccctgg tcctgatttc
    21301 gcctcccgtg ggcctgatgg acttcgagtc cctttcattg cccctccgtc ccgtcctggt
    21361 gatcgccggc gatgaggacg aattctgccc gccggattcc ctgcgctcgt ggctggacgg
    21421 tgcgggcgga tcccgggagg gcgtcgagct ggaaatcttt cacgggtgcg atcatttcta
    21481 ttggggaatc gagcggcgac tggccgatcg aatccgggaa ttcctccagc ggtgcgttct
    21541 gagaacgaaa gtctaaaagg gagcattttg gacttgggat ttgggcgatt ttgtgctagt
    21601 gaaccctcct tgtactcatc tgcctgaaat ccatctccga gtttgtgcaa atctgaagct
    21661 tccgcagtgg tcgagttcgt tcaatccgtt tgtgagggat gatgatggaa gaccgttttg
    21721 cgctgcaggg ggagtcgctg gttgtagacc ggcagaccgg gctcgtttgg cagaggggag
    21781 cgtccgggga tcgcatggtc tggaaagacg gcttccggta tgtcgaagat ctcaacggga
    21841 agcagttcgc aggtttcggc gactggcgct accccaccaa ggatgaactg gccaccctca
    21901 tcctccccga ggaagaccga aagaccgggc tttttgttca tccctatttc ggcactcaaa
    21961 ggaattgctg gacctcgacg gaaacgggcc gtcatcgggc ctgttacgtg gacttctact
    22021 acggggacgt ctacgtcatc gaggaagcct acgccaatca tttcatccga gcagttcgat
    22081 ccaaccctca atagcgtcga tttcggccgg ggcaggggat agtgcgcgca gccggggaaa
    22141 acaacggcgg agggaacgtc cctcccctcc gggtaattct cattgaaccg gagataccgc
    22201 agaacacggg gaacattgca aggacgtgtg ctgcaaccca tactcccctt catctcgtgg
    22261 aacccctggg tttccttctc accgatcggc acctgaaaag ggccgggctg gactattggc
    22321 cccatgtgcg gctgaaggtt cacagggatt tcgattccct gcaccaggag cttcgccatg
    22381 gccgatgggt gtgcttttcc gcgggcgcgc ccagggacta tttcgatttt cggttccggg
    22441 ccggagactg tctcctgttt gggccggaga cccggggcct tccttcacgg gtattggagg
    22501 ccgccgggcc gaacctgttg agaatcccca tggacaggac tcgcgtgaga agcctgaatc
    22561 tttccaccac ggtgggggtg gtgctcttcg aggccatccg ccagttgagc ttcgagtccg
    22621 ccgggtttct gtccgctttt gccccccttt ccggtccata accttttata aagacaggtg
    22681 ccgcaatgaa ctcgggttct tcccttcgag aaacgggcgc aagctccatc aagaggccgg
    22741 atagcgacgg actgctgcgc agattgtatg aagtctgccc ggagatcgtg cagttggggc
    22801 tcttccccgt cgtcctgggg aagggcagtc gctactgagt ggtcttcttt gaaagcaggg
    22861 actgctttcg aaagaacccg cccctcagct ccgtccggat ctgttgcaag aatccggaaa
    22921 gggattcggg cgacagggcg ccttcctggg aggaagacct gcgcagtgcg gtatccttgg
    22981 ccaagaatag gattcggtgc ggtcgaccgt cgagatcttc cggagagcaa acctgtcagt
    23041 ccaagttcgg agttgggagt cgtggccgaa atcgagtgga agggagccac ctggagagct
    23101 gcgtatggtg atctttccgt caaggaattg ttgaccatcc tcaaagggtt tggccccatg
    23161 gaaatattgg agttcgaaag accggggcgc gtccgcgggc aggtgagcct ttccctttcg
    23221 gccaagggcc ggaagcagat cactctctat tacctggaag tcttggggga gaggcgccgg
    23281 gggcgtggtc gcgaggccct ggaatggctc aggggaatct tcaagggaga gttgtacgtc
    23341 gaggacccgg ggattatcca cgtaaagaat cccaatgcgg aaagtctctt gttctggatt
    23401 gccatgtacc gggagggcct gatccaggcc atggagagcg agttctgcat cctgcggccc
    23461 ggcatgagag aaaaggaatt gacggaaatc gagaagaaac tggtggaggt ccggtagagt
    23521 tgggtcttat ccgtcaacag gcccttcatc ctacctcggc ctcctcccgt cgagggaggg
    23581 gagaaggctc tcccggtttc ggtggagagg accggagaga aaagaaggag gccatggcag
    23641 agtgctccgt cggctgaatg aaggaccctt tgcgtttcga tgggggaatt ccttgacttg
    23701 acccgggggc ctcgggtaat gagacctgga tcgggcattt cctgcaccgg ttggccgcaa
    23761 cggttgtgca ggctgtcgag ggaaaagagg aaaggagcgt ccctgtggga gcgatgaaga
    23821 tacgaggcac caaacgcgcc gtttttggcg aggaagaggt tgtgggggct ctcgaacggt
    23881 ttcaggattt tctcgaacgc aaccgcatgt ggagctacgg ggccctggtg gttttaatcc
    23941 tttgcgtagc ggggttttgg ggtttcaggt cctacgccga gtcccgggaa cgccgggcgg
    24001 cggaaaccta cgcaaggatc tccgtcacct tccccgacga cgaacaaggg gatgccggca
    24061 agtgggcggc cctcgtcccc gaattggaga aattcgtttc ggaacactcg gacagccctg
    24121 tgcggatcaa tgcatggatg gatctggcca aggcctgttt caacctgcga cgctacgaca
    24181 aggcggcaga ggcggctagc cgggccgtgg aagagtcggg tccccggtct cccctcttcg
    24241 gccttgcccg gtaccagctg gccctcatct accaggagat gggaaaggtc gacgaggccc
    24301 tggctcaatg gacggcgctc aagggcgatt ccatcaaggg gttcggccgg gagatcgagt
    24361 ggcgcctggc ccaactgcat gaaaaaaaag gggattacac cagggcgacg gaacagtacg
    24421 aggcggccct gaaagccccg ggctcgtatc cgcctgcccc gctgctctcg gaacaactgg
    24481 cctcgctcaa aacaaagacc ccgaccccga gcttcccctc gccgcagacc cctgccgccg
    24541 aagtcgcgga taagccccgg tcttgatttt ccttgacaga aggtggggga ttcgatattc
    24601 ggaaaggcgc gcaatttcag ggttttgagg aaactatggt gggtcgcgtt ttcttttttc
    24661 gatttttttc ccggatgaga ggctgttctt tcgaaatgcc tgcctgtggg tgctgcgtaa
    24721 tctagaacga aatgaacgag acaaaccatg ggtgggcagg gaaacggaac ccatggtttt
    24781 tttctttcgg aaaagccgtg gatttccaag ccgcggcttt ttttttgtgc aacgggacca
    24841 ggaggaagga ggtcgatggc ggatgattta cgatatcgag ttcgaaactt tgcctcggga
    24901 agcactcgaa gccatccagt tgaggcgttt gcagtcggtt gtggaaaggg tgtatgccac
    24961 ggtaccgttc taccgaaaga agttccagga atcgggggtc cgtcccggcg acatccaatc
    25021 cctggccgat ctcaggcggt tgcccttcac caccaagcag gacctgaggg acaactaccc
    25081 ctttgggatg tttgccgtcc ccatggacaa cgtggttcga atccatgcct cgtcgggtac
    25141 cacgggaaaa cccaccgtgg tcggctatac cgcccgggac atccatacct gggcggaact
    25201 gatggcccgg tccctggccg cggcgggagg cacccggggg gatatcatcc ataacgccta
    25261 cggatacgga ttgttcacgg gaggactggg aatccactac ggagccgagc ggttgggtgc
    25321 atcggtcatc cccatttccg gggggaatac caagcgacag atcctgatca tgaaggattt
    25381 cggacccacc attctcacct gcaccccttc ctatgccctg cacctggcgg aggtggctgc
    25441 cgaagccggg atcgatttca gccggctgaa cttcaaggcc gggatttttg gagccgagcc
    25501 ttggtccgag ggcatgaggg aagagatcga acgaaagctc cacctcaaag ccctggacat
    25561 ctacgggctg agcgaagtga tggggccggg ggtttccatg gaatgcatcg aggccaaaaa
    25621 cggactccat gtctacgagg accacttcat tgcggagatc atcaacccgg ataccggtga
    25681 ggtcctgccc cacggcgaga cgggagagtt ggtgttcacc accatcacca aggaagcgtt
    25741 tcccgtgatt cgttaccgta cccgggacgt tacctctctc atcccggagc cctgcgtctg
    25801 cggccgttct cacatccgca tgaagcgggt cagcggccgc acggacgaca tgctcatcat
    25861 tcggggagtc aacgtattcc cctcccaaat cgagagcgtg ctcatggaaa tcgaggaagt
    25921 ggaaccccac tatcaactgg tggtggaccg ggaaggaaat ctggacaccc tcaccgtcat
    25981 ggtggaaatt ggggaggaag ccttctcgga tgaggtccgg aaactccagg cgctggaaaa
    26041 aaagatttcc aagaacatca aggaatacct gggggtgtcg gcaacggtga agctcgtcga
    26101 gccgaagatg attgcccgca gcgaagggaa ggcccaaaga gtcatcgaca aacgaaagtt
    26161 ctaagcacaa aagggagcga gaccatgaaa gtggagcaga tctcggtctt cttggaaaat
    26221 aaggcgggaa gacttgcgga agtgactcga attctgatgg aatccggagt gaacatacgg
    26281 gccctgtcct tggcggacac ctcggacttc gggatcctgc gtctcatcgt caatgacaac
    26341 aacaaggcca aggaagctct aagagcccgc ggtttcaccg ttgggaagac ggacgtggtg
    26401 gcggtggagg tggaagatcg tcccggaggg ctccatcgca tcctggacgt cctgtaccga
    26461 gccaacgtca acgtggagta catgtatgcg tttgttcaac agagcggaag caacgccgtc
    26521 atcatctttc ggttcgataa cctggaagag gcggtgaagg tgctcacgga acacggtgta
    26581 ccggtcatcg aaccggagaa gctctattcc atttaacccc tcgtcgggcc ccgggtttct
    26641 cctcggcttc cccccgggat ccgagggagg ggttttccgg acgagcatcg gtccccccaa
    26701 gggtagagga ggtgtggaga gctcgaagca attggatagt gagacggggt gaacctgcca
    26761 tgtgcccttc ccgttgcgac ttgacgccaa accgacccca tcgatccgga ccggcgggaa
    26821 aaggttggat tcctgccgac cgggagccct tttgcctgcc cacgcgcacg cggaaaccgt
    26881 ccggtttggg aaaaccccga gtccgtcacg atcactggaa tggattcaca aggagaaccc
    26941 gatgattccg aggaattcca agataggaaa ggccctcatc ctgggcggga tggtcctgtt
    27001 gcttttggca tcgtccgtgg gttggtgcaa ggaaccgtac aagatcgggg gggtgttttc
    27061 cataacgggt cccaattctt tcctgggcga tccggaaaag aaaagcctcg agatggcggt
    27121 ggaggagatc aacgccaaag ggggcatcga cgggcatccg ctgcaggtga tcatctacga
    27181 taccgagggg gatccgacca aggcggtact cgccgtgaac aagctcatca gcagagatca
    27241 ggtaatcgcc atcatcggtc ccagcaacac gccgcccacc ctggccgtgg tgcccatcgc
    27301 cgagaaggaa aaggtccccc tcatcagctg tgctgcgggc atcaagatca cggaccccgt
    27361 caaaccctgg gtctttaaga cggcccagac ggacgccctt gccgtggccg ccatctacgc
    27421 gcacatgaag aagcagaacc tcaagaagat cgggatcctg accgttgaaa acgctttcgg
    27481 agaaagcggc aaggagcagc tgacaggtca ggcggctgcg gccgggatcc aggtcgtcca
    27541 gggggaaacc tttggggcca aggacaccga tatgacggcc cagctcacca agattcggac
    27601 cgctcagccc gacgccgtcg tctgctgggg cacaaacccg ggtcccgcgg tggtctccaa
    27661 gaatgtcaag cagatgaacc tgaagatgcc cctctaccag agtcacgggg tggcttcgcc
    27721 caagttcatc gagcttgccg gggacgcggc cgaagggatt ctccttccca cgggaaagat
    27781 cctggtggcc aagcaactcc cggattcgga tccccaaaaa gcggtcctcc tgcgatacat
    27841 cgaagattat gaaaagcggt accagacacc cgtttccggg tttggcggct atgcctacga
    27901 cgccatgcaa atcctggcca aggccctccg gggaaccgaa ggagacaagg ccaagatccg
    27961 ggacaacctg gaaaaggtaa cgggtcacgt gggggtcagc ggaacgttca acttttccgc
    28021 cacggaacac aacggtctgg gacccgatgc gtttgtcatg gtgcaaatcc agggcggcgc
    28081 ctggaagctt ttggcgccct gaggtccttg atccgccatg caggaatttc tccaatacgt
    28141 cttctcggga gtcaccaacg gcgccgttta tgccgtcatc gccctgggct tcagcatgct
    28201 gtaccgggcc acggggctca tcaactttgc ccagggcgag ctggtcatgt tgggggctct
    28261 ctgcctgatc tccctgtggc aggggctggg actgtctctg gtcctgtcga tcgtcctctc
    28321 cgtggggatc gtgtcggggg tgggaatcgc cctggaacgg ctggccatga ggcaggtgcg
    28381 aaagccccat cccatcgttc tggtcatcat cacggtgggg gcctccatct tctttcgcgg
    28441 cattgccatg atcctctggg gaaaagacgc ccgcagtgcc gggccctttt ccagcaacgt
    28501 tcccctggaa attgccggag ccaccctgct tccccagagc ctgtgggttc ttgccatcat
    28561 gctgggaatt gtggcggcct tgcacctgtt ctaccggaag accctcacgg gcaaggccat
    28621 ggaagcttgc gccatcaaca agaaggcggc ctggctcctg ggggtcccca gcgaccggat
    28681 ggtgcttctt gcgtttgcct tgagcgccgg gatgggagcg gtgggcggcg tcctcatagc
    28741 gcccatcacc atgagcagct acgacatggg gactcttttg gggctcaagg gattctgtgc
    28801 ggccatgctg ggcgggttgg gcagctcctg gggatccgtc ctgggggggc tcctcctggg
    28861 aatcctcgaa gccctcgggg tcggtttttt ctcgtccgcc ctcaaggacg ccatcgcttt
    28921 catgctgctc ctcttcatcc tgtacgtgcg gccgacggga atcctgggtg cccgggccgt
    28981 tcaccgtttc tgaggtttcc gatggaattg agatgctgat tcgcaacctg ctgtttgcag
    29041 ccgccattct cctgctggga gtgggggtca agaatcctta tcacctggag cttttgacct
    29101 ttatcggaat tcataccctg ctcgccctgg ggctcaacat gctcatgggg tacgccggac
    29161 agatctccct ggggcatgcg gccttttacg gactgggggc ctacgccacg gggattctca
    29221 ccgtccactg gcactggtct ccctggatca gcctacccgc caccctggcc ctggcggccg
    29281 cggtcgccct gctggtggcc cttcccatgc ttcggctctc cggtttctac ctggggatgg
    29341 gaaccctggg gttcggcatg atcgggttca tcctgtttcg ggggtggagc agcgtcacgg
    29401 gaggggattc ggggttggtg ggcatcccgt ccctggccgt gggacccttc acgttttctt
    29461 cgggagtccg ttacttttac ctggtgtggg gggcg
//
LOCUS       DAOOVC010000041        28184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1143_length_28184_cov_5.831384, whole genome shotgun sequence.
ACCESSION   DAOOVC010000041 DAOOVC010000000
VERSION     DAOOVC010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 28184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28184
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1143_length_28184_cov_5.831384"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1176)
                     /locus_tag="PLM79_07480"
     CDS             complement(142..1176)
                     /locus_tag="PLM79_07480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOV86189.1"
                     /translation="MMKSGGTGNVEGPGPRSDAPVRVVFPAEEAQRLDSFLSRVHPDF
                     SRNQFRKLILEGRVLVNGQPAKPSRELRAGEEVSVRFPVREELDELVPEPMHLDILFE
                     DEDLVVLNKAPGVVVHPGAGHSEGTLVHGLLAHCPRLALQGAPRRPGIVHRLDRDTSG
                     AMVIAKSERAYLDLVGQFKEHSVRKEYRVLVYGKMQEADGRIETLLGRHPMERKKMAV
                     VSKGGKPAVSRWRVETRWEEVSLLTVAIHTGRTHQIRVHLSHIQHPVVGDSTYGGGKR
                     RARLVRDEGLRELLAQVDRQMLHALRLGFRHPASRTPLEFVAPLPGDFSRLLESMGAP
                     AEFRAQKSRS"
     gene            complement(1432..2463)
                     /locus_tag="PLM79_07485"
     CDS             complement(1432..2463)
                     /locus_tag="PLM79_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOV86190.1"
                     /translation="MKWAWLILKVLLFAVCGFGGYLIAHDMESLSQFEWASWAGMLAG
                     MLFATVALSMEKLFKHMPLKVIIGGTLGLVLGLSVAKLVGYAFTGLENSTVRIAVYII
                     LSCIFGYVGIVLGTIKVDEMRLPNWSWIMRGGQRGGQSSEILDTSVIIDGRIADIVET
                     GFLSGTLIIPEFVLQELQHIADSPDPIRRVRGRRGLDIIKRLQQEKTVEVRIDRQDFG
                     NLSDVDAKLVALALRLNAKIVTNDYNLSKVAEVQGIRVLNINQLANALKPVVLPGEVL
                     RLQILKEGKEQGQGIAYLEDGTMVVVENASRHLGREVEVSVTSILQTTAGRLIFTTLK
                     DSDHTRHQH"
     gene            complement(2583..3062)
                     /locus_tag="PLM79_07490"
     CDS             complement(2583..3062)
                     /locus_tag="PLM79_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOV86191.1"
                     /translation="MFKIGDLAVYPAHGVGRIESIETKSIAGNKQDFYIMRILENDMK
                     IMIPIRNAEAVGLRQLIDTNEIPRVYGILRSKEVSVNGGTWNRRYREYMEKIKTGSIY
                     ELAEVLRDLTVLKNDKELSFGERKMLDTARTLLLKELSIVREVSEEIVDQEIREILE"
     gene            complement(3329..3982)
                     /gene="pth"
                     /locus_tag="PLM79_07495"
     CDS             complement(3329..3982)
                     /gene="pth"
                     /locus_tag="PLM79_07495"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOV86192.1"
                     /translation="MGDRFRLLGLGVTMSGIALSQEGILVVVGLGNPGSQYESTRHNI
                     GFRVVDALALRFGLHFQERKFQAAWASGRWEDRKILLVKPLGFMNRSGDPVSEVLRYF
                     GIAPSRMLVVHDDLDLPFGHVKLVRGGGAGGHKGVHSISQSIGHVRFPRLKLGIGRPM
                     RGESVEAYVLEPPYAEQREVFDRMILHGARVAETALSSGLEEAMNQYNRREGQILQS"
     gene            complement(3989..4594)
                     /locus_tag="PLM79_07500"
     CDS             complement(3989..4594)
                     /locus_tag="PLM79_07500"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOV86193.1"
                     /translation="MDIELNAQVRKVGGKGVARRLRREGRVPAVLYGPKTPSQLLSLS
                     AKSLEKLLSGMGEESRLLQLKIEDGSEVQNRQVLVREVQVHPVRRRFLHVDLYEVPLD
                     HPIVVEVHVELMGESVGVRKGGTLNLIRRTLSVRCLPGEIPEKIELNVADLDLGGSVH
                     VEDLIGRVPYELVDDPSFAVVNVSSPEEAGKESEGAGGEEA"
     gene            complement(4666..5604)
                     /locus_tag="PLM79_07505"
     CDS             complement(4666..5604)
                     /locus_tag="PLM79_07505"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOV86194.1"
                     /translation="MESRLVILCGNSNWPLAQKVCAHLGLPPCSAIVGRFSDGEVRVD
                     IPENVRGKDVFILQSTCPPVNENLVELLVMMDALKRASAARINAVIPYYGYGRQDEKD
                     KPRVSITAKLIANLLMEAGADRIIAVDLHSDQIEGFFSIPVDHIHGTQVLADDLRKRL
                     GGGEIILAPDSGGVGRARRFAELLGAGLAMVDQRTEENEPFARIVGQVGGKRVIIVDD
                     MVDTGRILLRAVYGAVAAGAASVEACCVHAVLSGDAVDKLEASPMDLFTVTDTIPLTT
                     RSERSGKLRTVSMAPVLAEAVRRVHDGVSVSAMFVR"
     gene            complement(5679..5753)
                     /locus_tag="PLM79_07510"
     tRNA            complement(5679..5753)
                     /locus_tag="PLM79_07510"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5719..5721),aa:Gln,seq:ttg)
     gene            complement(5771..6640)
                     /locus_tag="PLM79_07515"
     CDS             complement(5771..6640)
                     /locus_tag="PLM79_07515"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOV86195.1"
                     /translation="MDQRTITSRTPGKINLWLEVLGKREDGYHELSSLMLPIGIHDTL
                     TLRVGGERGVRVRCGHPLVPCDRRNLVVRAVEAYLSETDFRQGVDIHLEKGIPVSAGL
                     GGGSADAGAALQMIHRLVPEPLPEERLLSIARGIGADVAFFLDPYPALASGIGEKLEK
                     VPGLPDYPLVLIKPDVAVSTREVYGSLKLTRGVSRIKLQAFVAKPWEVQGFLENDLET
                     VTFSRLPELGAIKSWLLEHGALGALMSGSGPTMFGVYPSMADAQRAEALAGKVWGRYW
                     VAVTSVMAAGNSP"
     gene            complement(6750..7178)
                     /locus_tag="PLM79_07520"
     CDS             complement(6750..7178)
                     /locus_tag="PLM79_07520"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HOV86196.1"
                     /translation="MDEKEEKGFVIKDRRKVSGEDLDRQEKKGGGDEGREKEDARAKD
                     EFDKVSRDDRQGEKAALPEVTLSAFIFSLSSSALVHLGEIPEPDTKATRVDLPMARQI
                     IDTLGLLQEKTRGNLDPNEDRLLKSVLYDLRLRYVQKSKS"
     gene            complement(7200..8780)
                     /gene="serA"
                     /locus_tag="PLM79_07525"
     CDS             complement(7200..8780)
                     /gene="serA"
                     /locus_tag="PLM79_07525"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOV86197.1"
                     /translation="MRILVSDNLAEVGIEKLRSVPEFQVDVNTGLSHEELCRVIKDYD
                     ALVIRSATKVRADVIEAAEKLKVVARAGIGLDNVDVEAATKRGVVVMNTPDGNVITTA
                     EHTIAMLLSMSRNIPQAMHSLKSGKWEKKRFQGKEVFHKVLGVIGIGRIGRVVADRAL
                     GLKMKVIAYDPYISAEVIHKMGIEGVSLDELLARSDYITIHTPMTPETRGILNEEAFK
                     KMKKGVFVLNCARGGVVDEEALYEAIRSGIVAGAALDVFTKEPPTDNPLLTLDQVVVT
                     PHLGASTEEAQENVAMAVADQIMDYLLKGTIRNAVNAPNLDGALLAHLRPYLVLSEKL
                     GSVLAQITRGAIKEVSIEYIGEVTAMDTRPLTFSIMKGMLTPVLGDMVNFVNVPLHIK
                     ERNIKVREMVTSEAEDFTNLISVHLKTSEDQNLVAGTMFGKKDPRLVRINDFRLEAAM
                     EGHLVLIYNIDTPGTIGAIGTCLGRHNINISMMDVGQVLERGQNIIFLRTDTSVPKHV
                     VAELLSMENVNVVQRLEL"
     gene            complement(8961..10406)
                     /locus_tag="PLM79_07530"
     CDS             complement(8961..10406)
                     /locus_tag="PLM79_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOV86198.1"
                     /translation="MLKENAKERLLLILSLAGFLLSALTGMSEKADWLEVLCTTASDG
                     CRETAAFTLAWLPVWAWGLVFYGVLVLTFSRAKKWMIWVVSAGMGVELSLAWVLFSKK
                     LICVFCLGNLAVVILLVLLSFDRKRVWEMVSFCLFFWVLSGSVLIHGPESAASASGAD
                     RTEAPVAAAEVAGQVITMQELEAPFLNRIHEMQHEIYRLKKQRLDQLVADQVLHKEAV
                     SRGITVQQLVTEEVLSKGASVTDEEVEKYLQENRGRMADWKGTQEELRDRVRSYLQQQ
                     KNLQGVMEFSKTLEPKYGVIVHLKEPSPPRTRIDVEGSPSWGPKDAPVAVVEFSDYQC
                     PACRQAHEVVLKVREKYGNRLRWVFKDYPLKRHKDAERAAEAAHCANDQEKFWEYQDL
                     LYSTTEELSGEKLELLAGKIGLAMGSFKACLDGGKYRAKVQKDIEDANKAGVDRTPTF
                     LINGKMYTGAIPAERFSLLIEEELKAAAKKP"
     gene            complement(10519..11973)
                     /locus_tag="PLM79_07535"
     CDS             complement(10519..11973)
                     /locus_tag="PLM79_07535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOV86199.1"
                     /translation="MNRWFRSVRGRRFLVPVVVWLLCLSLAMPGPAVWALGLGDEKEL
                     GRKILGQIRAHIPLVEDPAIVSYVQSVGNRIVRELGTTSYEYQFYVVDSSVPNAFAIP
                     GGYIFVFRGLIEFMAGEGELAAILSHEMAHIQGRHVHRQMDAGKVLTAAALAGVLAGV
                     LLGSATGNASASPAVAMGSVAGSQALGLKYSRENEQEADQLGLSYLSSAGYPPRDMVT
                     VMQRLNDVKLRSNSRIPSYLSTHPDTGDRIQYLTDLCNKYPQKQASKNSKGRSSDFQL
                     MQAGLIAEYGDTKVARDRFDRPNPTLAETYGLARLYLRLGRTEEAVSYLLEAARKEPG
                     SPFILCTLGNAYFRQGRLQDAQRVLQTCLKLDPSNSMARYQLALVLQDQGNREEAVAQ
                     LLKIEPMSATFPEIDNRLGVLLGQVNRMGEAHYHLGRYYESKQDFKVALFHYEKAKAM
                     LKDSVQKIDELNERIKEMKQYEKEAMMRKREKRR"
     gene            complement(12119..12550)
                     /gene="rsfS"
                     /locus_tag="PLM79_07540"
     CDS             complement(12119..12550)
                     /gene="rsfS"
                     /locus_tag="PLM79_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOV86200.1"
                     /translation="MSLTQGPAKANREGEGLGAREKAALCAREAERFLAQDLVLLDVS
                     GLSSFADYFILCSGKSGRQVQSIAENLEARLREHGLRPLGVEGRQEGHWILMDYTDVI
                     IHIFYEPVRPFYDLESLWSEAKRIRTGPTGGPVQCAASDPS"
     gene            complement(12547..13233)
                     /gene="nadD"
                     /locus_tag="PLM79_07545"
     CDS             complement(12547..13233)
                     /gene="nadD"
                     /locus_tag="PLM79_07545"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOV86201.1"
                     /translation="MRLGILGGTFDPVHVGHLRIAEEAVEFLALDTLLFIPAAIPPHK
                     GATGILPFEYRYEMLRLGIQGNPRFQVSALEKDFPGKSYTVVTLGRLREQSPPDAELF
                     FLVGMDAFFEMGSWWHYRELFRLASIVVLHRRGYSEGSVGDFLRAKVSAGYFRGREEL
                     KFEDPALKPVYLLDNTLIEVSSTRIRRLVAQGRSIRYLVSEAVMGYIQQNRLYRLEAG
                     KPGEKGSVGS"
     gene            complement(13275..14525)
                     /locus_tag="PLM79_07550"
     CDS             complement(13275..14525)
                     /locus_tag="PLM79_07550"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOV86202.1"
                     /translation="MRDEILTMARDARAAAHEMAKVNSDRKRKALEAVAVALEKSRSR
                     IGEANDKDVREALKKGLSGAKVDRLRLSDKVIQEMVGGLREVAALADPVGEVTRMWVR
                     PNGLRVGRMRIPLGVIGIIYESRPNVTIDAAALCVKAGNAVILRGGSEAFESNRCLAD
                     ILQEVLEAEGLPATAVQMVGTTDREAVLEMLRLEEFIDVMIPRGGEDLIRFVTEHARM
                     PVLKHYKGVCHIFVDREADLDMAEKVCMNAKVQRPGVCNAMETLLVDEAVAEKFLPRM
                     IESLRKEGVEIRGCPRTRQLVGDCLPAGEEDWSAEYLDLILAVRVVSGMDEAIRHITR
                     YGSQHTEAILTENFERAHRFLQEVQSSLVLVNASTRFNDGFQLGLGAEIGISTSRLHA
                     FGPMGVEELTTTKFVALGNGQVRT"
     gene            complement(14518..15717)
                     /gene="proB"
                     /locus_tag="PLM79_07555"
     CDS             complement(14518..15717)
                     /gene="proB"
                     /locus_tag="PLM79_07555"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOV86203.1"
                     /translation="MERGVCVTTEGTDRKALFGRCRRLVIKVGSAVLTGPQGLNRVMI
                     HRLSDQMAELRERLPELGIVVVSSGAVASGVRKLGLAERPRTIPQKQAAAAVGQGALM
                     EAWEAAFEKYDLHVAQVLLTREDLAHRHRYLNARNTLETLLDWRILPIINENDTVVVE
                     EIKFGDNDQLSALIAGLIGADLVINMTDTEGLYDCDPRLNPSANLIPVVHRVDSRLLA
                     CAAPRPGTVGTGGMRSKLNAARKCLASGIPMIIAPGRERDLLLRLFQGEPLGTLFLPR
                     ERLYQGKKVWLANLPKPAGNLTLDEGAVAALQRRGKSLLPIGVKAVHGTFGVGAPVRC
                     LDPQGRVIGVGLTNYKSGEIDLIKGHRSEEIESIIGYRHSDEVIHRNNFVVISEGIED
                     FEGGSDA"
     gene            complement(15895..17007)
                     /gene="obgE"
                     /locus_tag="PLM79_07560"
     CDS             complement(15895..17007)
                     /gene="obgE"
                     /locus_tag="PLM79_07560"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOV86204.1"
                     /translation="MKFVDEVKITVRAGKGGNGCVSFRREKYVPRGGPDGGDGGNGGS
                     VLLTASRRKHTLLDFHYRHLFRAPAGKHGQGQNKHGRGGRSLVLEVPVGTLVKDAVTG
                     EVLVDLDEPGQSWTAVRGGTGGRGNARFVSSVRQVPREAEEGREGEERDLVLELKLMA
                     DAGLVGLPNAGKSTLIAAVSAARPKIADYPFTTLVPHLGVVRCGEFEPFVWADIPGLI
                     RGAHQGTGLGIRFLRHIDRTRLLVHVIDGSALPSEDLLDPYRQVESELSRYSEEMGKK
                     LRIIVINKTDLLPDPRERQRIADVYATTGHKVLLVSALQRRGTDALVRLVAETLSKLR
                     DAQRISAGFPEESEGCGGTEGGRQGPGTRRRGPDFD"
     gene            complement(17352..17618)
                     /gene="rpmA"
                     /locus_tag="PLM79_07565"
     CDS             complement(17352..17618)
                     /gene="rpmA"
                     /locus_tag="PLM79_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOV86205.1"
                     /translation="MAHKKAGGSSRNGRDSAGQRRGVKKYGGEFVRAGNILVRQLGTK
                     FHPGKNVGVGRDYTLFALIDGIVSFEYRDKTRQQISVYPIPTQS"
     gene            complement(17677..18006)
                     /gene="rplU"
                     /locus_tag="PLM79_07570"
     CDS             complement(17677..18006)
                     /gene="rplU"
                     /locus_tag="PLM79_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOV86206.1"
                     /translation="MYVVFQSGGRQYQAKLGTVVKVERIPGSVGDRVTLDRILLCARE
                     DQVEVGRPLLDGLAVQGRIVEQGRGKKILILKHKRRKDYRKRQGHRQAYTALLIESVA
                     RGAAEQA"
     gene            complement(18127..18255)
                     /locus_tag="PLM79_07575"
     CDS             complement(18127..18255)
                     /locus_tag="PLM79_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86207.1"
                     /translation="MIKSPCLSCDRAKEDKNKCLENCEKLREYQELILKQGIYATM"
     gene            complement(18435..19187)
                     /locus_tag="PLM79_07580"
     CDS             complement(18435..19187)
                     /locus_tag="PLM79_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOV86208.1"
                     /translation="MKIITHSDFRQVYVSDPAAAPGRIEAILDAIGDEVTLEEAIPAG
                     WDDIEAVHTARHIENVTRRGLYSISALAAGAAIQAATLGLEEPSFALVRPPGHHASAD
                     SSWGFCYFNNMAVALDHLRRAKRITKAYVLDFDLHFGDGTVNILEPKGYVTIHNPEDD
                     DRESYLKEVELSLQRAQMDIIGVSAGFDNHRLDWGGLLNTEDYQVMGYLVHETAVKRG
                     IGCFGVLEGGYNHSVLGQNVRAFLRGLRGLKP"
     gene            complement(19448..21034)
                     /locus_tag="PLM79_07585"
     CDS             complement(19448..21034)
                     /locus_tag="PLM79_07585"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOV86209.1"
                     /translation="MGGDAFSKADLKQMERQGITPAQVRDQLAICRSASFSRLVRPCT
                     LGDGIRLLSAEERERAVERQRIAAQAGRFQKFVPASGAATRMFQSLLQIFHMPHFLER
                     EELQCRVRQGVSVACDFLAFLDGLPRFAFYEDLEKAVVREGTTLAALIQGSQFRSLLE
                     FLLTPRGLAYGSLPKGLLKFHRYPTESRTAFEEHLVEAARYIRDARGSCRLHFTISPE
                     HEEWFRFLHEKAQSVHGVPEGVRYEVGFSFQKRSTDTIAVDRNKRPFRDRSGRLVFRP
                     GGHGALLENLRDLEGDLIYIKNIDNVVPDRLKEPGIFWKMVLGGYLVEVQERVHEWVG
                     GLQQDPSADFIRSAARFAAEDLLLDLSPAFDKLSAGEQRDRLIEVLDRPIRVCGVVRN
                     VGEPGGAPFWVKGSDGRLSIQIVEKAQVNLEDPDQRRTWESSTHFNPVDVVCAVRDFE
                     GRPYDLRKYVDPRAVILTRKSKDGRELEALELPGLWNGAMAGWITLLVEVPRITFNPV
                     KTLFDLLKPEHSSEPGPSAQ"
     gene            complement(21126..22121)
                     /locus_tag="PLM79_07590"
     CDS             complement(21126..22121)
                     /locus_tag="PLM79_07590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOV86210.1"
                     /translation="MVHKRWSWTYGCLFGIALGMLLISGGEGVAQEKSAAKKEAAKVN
                     DVVISQDELDAALKRVENQMVAMGHPASVGQMPEIKNQVLQNLIDRELLYQDSQKQGI
                     QTEEAEIDEQVNALKARFPGDKEYRAALQRLNLSEQDLRAQFGREISVQRLIDLKVAA
                     KVSVSEPEIKAFYDSNPDLFKSPEMVRARHILVKVEPKGSDADKAQARGKMEQIAARI
                     KKGEDFAAVAKETSDCPSGARGGDLDFFKRGQMVPQFEAAAFSLREGEISEIVETQFG
                     FHVIQVTGKKPASVASLEETKEKIGQHLKQEKTDQQVSRYVTQLKSDANIQVFAQ"
     gene            complement(22405..23475)
                     /gene="secF"
                     /locus_tag="PLM79_07595"
     CDS             complement(22405..23475)
                     /gene="secF"
                     /locus_tag="PLM79_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOV86211.1"
                     /translation="MQWIRPDIDINFVGMRFKAFVLSSLLILLSLAAMVWRGGLNLGV
                     DFAGGTLVQIQFQRSTSPDEIRNALSDLGFAQSAIQQVGAASDNEYLIRTDLTTSELA
                     DLSRRVEESLGKAYGQGQATVRRAEMVGPKVGHDLRQKALFAIYYALLFIAVYVSGRF
                     ELKWATSGMMVGVLIIGVYLLEAIWISVSYLILGALVVTMGFCWVLKLPYALAAVLAL
                     VHDVLITVGIFAVTNKEFTLEILAALLTIVGYSLNDTIVVFDRIRENVRKVRKDEFKK
                     LINDSINQTLSRTILTGGTTLLALVFLFLFGGTVIHDFAFAMIIGIVVGTYSSVFVAS
                     PLLIFYQDWAGGGAARPRKKPA"
     gene            complement(23503..25104)
                     /gene="secD"
                     /locus_tag="PLM79_07600"
     CDS             complement(23503..25104)
                     /gene="secD"
                     /locus_tag="PLM79_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOV86212.1"
                     /translation="MKNLKWRVVLVVVVLLGGLVYLTPTFTSTLPPWWSKVLPKEKIH
                     LGLDLQGGMHLILEVDTDKAVENYADRVAEEMKETFRKERIPFTKVERTKKWDVVVQL
                     AGAEAQTPLAKLVEKDYPVMKLVGAEGVAEGAMQVTLTLQDFQVNHIRKMSSSQALET
                     IRNRVDQFGVTEPDIRPQGEDRILIQLPGVKDPQRAVALIGKTALLEFKLVAEDVTPQ
                     DVKEGKTPAGVKVFPMKHVDSKTGAVDMTQIALKDRTVLTGEALTTAEVRLDSQYSTP
                     YVGLEFDSEGARIFERVTGENIKKRLAIVLDGTVYSAPVIQDKISGGKASITGSFTME
                     EARDLAIVLRAGALPAPVKILEQRTVGPSLGQDSIRKGLLASVLGGLVTIVFMVVYYR
                     ASGLIADLALILNIPLIMACLAAFGATLTLPGIAGIILSVGMAVDANVLIFERIREEL
                     RLGKTLRAALETGYSRATLTIFDSNITTLIAAVVLFQFGTGPVRGFAVTLTIGIVTSM
                     FTAIVVTRIIFDYLLLQRRVRTVSI"
     gene            complement(25217..25558)
                     /gene="yajC"
                     /locus_tag="PLM79_07605"
     CDS             complement(25217..25558)
                     /gene="yajC"
                     /locus_tag="PLM79_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOV86213.1"
                     /translation="MDWVGVAHAMGSMGQGDGGQGAAGGFAAFAPLIFMVVIFYFLLI
                     RPQQKKQKEHKAMLSGLQKGDVVITQGGILGRITGLTDTVVTLEIADKVRVKIQRPYI
                     VGLVSKGEVAE"
     gene            26187..27350
                     /locus_tag="PLM79_07610"
     CDS             26187..27350
                     /locus_tag="PLM79_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86214.1"
                     /translation="MSIPRDTTTADQVMNDHVDLARLQSEIRHNCDISDANHAGLFSI
                     CGLLLRLRDLYKWEHSTPPWQEPEPADLMEWIDARETRWEEVLHRPWASLTVGDETFQ
                     PFEIASINARLAPHGLIYGAGYAVGMKPSFFLGEILETRRLGSLTIHVVGKEWIRDLY
                     AAPAMRQGEQIFARRTPMLFFLWDQVMEMRPSAREPLSYAFAQYGLNVDSLRRLPAEM
                     GPRLEPVAASELETWIYHEVGEASDTTFQGLQWQEIVSSYANSPVEIFTRVIKDLLSD
                     THPQGLLGHIIRHGIKPSLAFYVSFMRPFTRVLFPEIRVAFGDFIRRDDWTGIERARA
                     AGYEKARHNGLELIRLHEAGKGHDSQRTREKIISHLIEPLGVLGTLTSREDEP"
     gene            27383..>28184
                     /locus_tag="PLM79_07615"
     CDS             27383..>28184
                     /locus_tag="PLM79_07615"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV86215.1"
                     /translation="MKTWVADLLICPACLPREHGLILRTEERARDEILLGSLDCPGCA
                     SRYPITDGIADLLPASFGSNGAIGGKYEQSPVVSSYLWSHYADLFGDPDVLKSYEQWG
                     ALVEPVPGLSLDMGCATGRFAFELASRSAGVVGIDSSRNLVRAARELMIHRRLRFALR
                     LEGQIRRVQTIEVPSSWDCDSIDFLRADACNPPFRTGTFHGVASLNLLDKVPRPLRHL
                     QEADRVAGKQDAQLLVSDPFSWSEEVADPKDWLGGKTRGKYSGSGWENL"
BASE COUNT         5459 a         9091 c         7822 g         5812 t
ORIGIN      
        1 catcgcgaga gcgatgtgac ccgcgagttt ctcgggaagt tgaagcagct caaaaagcgc
       61 atcgaaaact aagcgggtct tcccaggagt cggggggggc ggcgcggtgt gggagggcgc
      121 cgtcggaaga tctgtgaggg atcaggacct cgacttttgc gcccgaaatt ccgcgggagc
      181 ccccatggac tcgagaagcc gggagaagtc cccgggcagc ggcgcgacga actcgagggg
      241 ggtccgggat gccggatgcc gaaatcccag gcgcagggcg tggagcatct gccgatccac
      301 ctgggcgagc agttccctca gaccctcatc cctgaccaga cgggcccttc tcttgccccc
      361 tccgtaggtg gaatccccca ccaccggatg ttgaatgtgg ctgaggtgca cccggatctg
      421 atgggtacgt cccgtgtgga tcgccacggt cagcagggaa acctcttccc accgggtttc
      481 gacgcgccac cgggagacgg cgggcttccc gcccttggac acgaccgcca ttttctttcg
      541 ttccatcgga tgccgcccca gcagggtttc gatgcgcccg tcggcctcct gcattttccc
      601 gtacaccagg acccggtatt cctttcggac ggagtgttcc ttgaactggc caacgaggtc
      661 caggtaggcc ctctccgact tggcaatcac catggctccc gaggtgtccc ggtccaggcg
      721 atgaacgatg cccgggcgcc ggggcgctcc ctggagggcc aggcgcggac aatgggccag
      781 gagtccgtga accagggtgc cttccgagtg gcccgcaccg ggatggacca cgacacccgg
      841 cgccttgttc aaaaccacca ggtcctcgtc ctcgaacaag atatccaggt gcatgggttc
      901 gggaaccagt tcatcgagct cttcccgaac gggaaaccgc acggagacct cctcccccgc
      961 acgaagctcc cggctgggct tggcaggctg cccgttcacc aggacccggc cttcgagaat
     1021 cagcttgcgg aattggttgc gggaaaaatc cggatgtacc cggctcaaaa atgagtccag
     1081 cctctgagct tcttcggcag gaaaaaccac tcgcaccgga gcgtcggatc tcgggcccgg
     1141 accttccacg tttccggttc ctcctgattt catcatgatt ctggactgtc ctgaaaaagg
     1201 aaaggctcct catgaatcgc cgtccctgcc cgggccggtg tcccggccct cctcccggga
     1261 ccccgcaccc gcctcccccg ggacccgccc caaagaagag ccgggtccct cctaggatcg
     1321 aacgtccttt tccggggagc cgcattgcgg gcacggcatc cgggcttaaa cgaagacggg
     1381 cgggatgacc cgcccatctt cgccgtactt cggtcgcctc ggggaaccgg atcaatgctg
     1441 gtgtcgcgtg tggtccgagt ccttgagcgt cgtgaagatc aatcgccctg cggtggtctg
     1501 caggatgctc gtgaccgaga cttcgacctc tcttcccaaa tgccgactgg cattctccac
     1561 caccaccatc gtgccgtcct cgagataggc gattccctgc ccctgctctt ttccctcctt
     1621 gaggatctgc aagcggagga cttcaccggg gaggaccacc ggtttcaggg cgttggccag
     1681 ctggttgatg ttgagaactc gaatgccctg gacttccgcc actttggaca ggttgtagtc
     1741 gtttgtcacg atctttgcgt tcaggcgcag ggccagagcc accagtttgg cgtccacatc
     1801 gctcaggttt ccgaaatcct gccgatcgat gcgaacctcc acggtcttct cctgctgaag
     1861 ccgtttgatg atatcgagtc cccgacgccc ccgcactcga cggatgggat cggggctgtc
     1921 ggcgatgtgt tgcagttcct ggagcacaaa ctccggaata ataagggtgc cgctcaggaa
     1981 ccccgtctcc acaatgtccg caatgcgccc gtcaatgatg acactcgtgt ccaggatttc
     2041 cgaggactgg ccgcccctct ggcccccgcg catgatccag gaccagttgg gcaatcgcat
     2101 ctcgtccact ttaatagttc ccaaaacaat gcccacgtat ccaaaaatgc atgagagaat
     2161 gatgtaaacg gcgattcgga ccgtgctgtt ttccagaccc gtaaacgcat agcccaccag
     2221 ttttgccacg gagaggccca gcacaagacc cagcgtgccg ccgatgatga ccttgagcgg
     2281 catgtgtttg aaaagctttt ccatggaaag ggccacggtg gcgaagagca tgcccgccaa
     2341 catgcctgcc catgacgccc attcaaactg cgaaaggctc tccatgtcat gggcgatgag
     2401 ataacctcca aatccgcaca cggcaaacag caacaccttc agaatcaacc acgcccactt
     2461 caatccggtt cacccccttt cccttggaat ggcctctcga gaaggaccgt ctcaggaggg
     2521 tgcgcccccg atcagggtcc aatgcagtca aagtcgggac cccttcggga ggcataaaag
     2581 catcactcga ggatctcccg aatctcctga tccacgatct cctcgctgac ttcccgtacg
     2641 atggaaagtt ctttgaggag cagggtcctg gccgtatcca acattttgcg ctcgccgaag
     2701 gagagttcct tgtcgttctt caacaccgtg aggtcccgca acacttccgc cagttcgtag
     2761 atggagcccg tcttgatctt ttccatgtac tcgcgatatc gacggttcca ggtgcccccg
     2821 ttcacggata cttccttgct tctcaggatt ccgtacactc ggggaatctc gttggtatcg
     2881 atcagttgcc tcagacccac cgcttccgca ttgcgaatcg ggatcatgat cttcatgtcg
     2941 ttttccagga ttcgcatgat gtagaaatcc tgcttgttcc ccgcgatgct ttttgtctcg
     3001 atggattcaa ttctgccaac accgtgagca ggatatacag ctagatctcc tattttaaac
     3061 attatttcct ccagagttcc tagccattcc agcggatttt gcagtatttg aggcaagatc
     3121 gactcaattt tatcactagt tttggggagt gcaaagccta atcacaagca ccaaggctgg
     3181 gatggaggta tctggcatgg tatgtgaaac aacgagaccc ggtccgtcac ggaagcgctc
     3241 aaacggtgca ggttttcgaa gagtcggccg ggatggatct ccacggggtt ggaaagtcaa
     3301 gaaaggagtg ccttgggcga gtattccctc aactctgaag aatctgccct tcgcgccgat
     3361 tgtattggtt catggcctct tccagaccgg acgaaagagc cgtctccgca acccgggccc
     3421 catgtagaat catccggtcg aacacctccc gttgttccgc atagggaggc tccaggacat
     3481 acgcttccac cgactccccg cgcatgggcc ttccgattcc cagcttcaga cggggaaagc
     3541 ggacatgccc gatgctctga ctgatggaat ggaccccctt gtgccctccg gccccccctc
     3601 ccctcaccag cttcacgtgt ccgaagggga ggtccaggtc atcgtgaacc acgagcatcc
     3661 gggaaggggc aatcccaaaa tatcgaagga cttccgacac cgggtccccg cttcggttca
     3721 tgaagccgag gggtttgacc aacaggatct ttctgtcctc ccaccggccc gacgcccaag
     3781 cggcctggaa tttcctctcc tggaagtgga gcccgaaccg gagtgccagg gcatccacga
     3841 cccgaaagcc gatgttgtgg cgggtcgact catactgact cccgggattc cccaacccga
     3901 ccacaaccag gattccttct tgggacaaag cgataccgct catggtcacg ccgagtccga
     3961 gaagccgaaa ccgatctccc acgagggatc aggcctcttc ccctcccgcc ccctcggatt
     4021 ccttgccggc ctcttccggc gaggagacgt tcacgacggc gaagctgggg tcgtccacca
     4081 actcataggg aacccgaccg atgaggtcct ccacgtgaac ggagccgccc aagtccagat
     4141 ccgcaacgtt cagctcgatc ttctcgggaa tctctcccgg cagacatcgc acggacaggg
     4201 ttcgccggat gaggttcagg gtgccgccct tcctcacgcc gacggattca cccatcagct
     4261 ccacgtgcac ctccaccaca atggggtgat ccaggggcac ttcgtagagg tccacatgga
     4321 gaaatcggcg acgcacgggg tgtacctgga cttcccggac cagcacttgt cggttctgga
     4381 cctcgctccc gtcttcaatc ttcaactgca ggagcctgct ctcttctccc atgcccgaga
     4441 gaagcttttc gaggctcttg gcggaaagag agagcagttg agaaggcgtc ttgggaccat
     4501 acagcactgc agggactctg ccctccctgc gcaaccttct cgcgactcct ttgccgccga
     4561 cttttctcac ttgagcgttg agttcgatat ccatttttcc ttgtctgcct cccaaaggaa
     4621 aggagcactc cgtaaagggt tgcccaaaat tgccggttac gagctttacc ggacaaacat
     4681 cgcgctcacg gacactccgt catgaacgcg acgaacggct tccgccagca ccggggccat
     4741 gctgaccgtt ctcagcttcc cgctccgttc ggaccgggtc gtcaagggga tggtgtccgt
     4801 gacggtgaat aggtccatgg gggaagcctc gagcttgtct acggcatctc cggaaagaac
     4861 ggcgtgtacg caacaggcct ccaccgacgc ggcccctgcg gcgacggccc cgtaaacggc
     4921 cctcagaagg attcgaccag tatccaccat gtcgtccacg atgatcaccc ttttgccccc
     4981 gacttgaccc acgatgcggg caaagggctc gttctcttcg gttctctggt ccaccatggc
     5041 cagacccgcc cccagcaact cggcgaacct cctggccctc ccgacccctc cggaatcggg
     5101 agccaggatg atttctcctc cccccaatct cttgcgcagg tcgtccgcca ggacctgagt
     5161 cccgtgaata tggtccacgg ggatgctgaa aaacccttcg atctggtccg aatggagatc
     5221 caccgcgatg atgcgatcgg cgcccgcttc catcagaagg ttggcaatga gcttggccgt
     5281 gatggaaacg cggggcttat ccttttcatc ctgccgaccg tacccgtagt aggggatcac
     5341 tgcgttgatg cgggcggcgg aagccctctt gagggcgtcc atcatcacca ggagttccac
     5401 caggttttcg ttgacggggg ggcaggtgga ctggaggatg aagacgtcct ttccccgaac
     5461 gttctcgggg atatcgaccc ggacttcccc gtcgctgaac cgtcccacaa tggccgaaca
     5521 gggtggaagc cccaaatgag cgcaaacctt ttgagcgagg ggccaattgg aattcccgca
     5581 caggatcaca agtcggcttt ccatgcgcta ctcgaacctc atccctgaaa gattcgcacc
     5641 cccacgcacc ccaggtaccg ggaagactcg agaagggctg gctggggtgg gaggattcga
     5701 acctccgaat gcgggttcca aagacccgtg cctttccgct tggcgacacc ccaaactctt
     5761 gcggcaggcc ctacggggaa ttcccggcgg ccatgacgga agtcacggca acccaatagc
     5821 gaccccagac cttcccggcc agcgcttcgg ccctttgggc atcggccatg gacgggtata
     5881 ccccgaacat ggtgggcccg ctgccgctca tgagggcccc caaggcaccg tgttccagga
     5941 gccagctttt gatggcgccc agttccggga gccgggagaa ggtgaccgtc tccaggtcgt
     6001 tttccagaaa cccttggacc tcccagggct ttgccacaaa ggcttgaagt ttaattcggc
     6061 tcacccctct tgtcaacttc aaactgccgt agacctcccg cgtggatacg gctacatccg
     6121 gcttgatgag caccagaggg tagtcgggaa gacccgggac cttttccaac ttttctccga
     6181 tccctgaggc caacgccggg tacgggtcca aaaagaacgc cacgtccgca ccgatccccc
     6241 gggcaatgga aaggagcctt tcctcgggca ggggttcggg cacaagccga tggatcattt
     6301 gcagggccgc accggcgtcg gcgcttcccc ctcccaaccc ggcactcacc gggatcccct
     6361 tctcgaggtg gatgtcgacc ccttgtcgaa aatccgtctc ggacagatat gcttccacgg
     6421 cccgaaccac caggttgcga cgatcgcagg gcacgagcgg gtgcccgcac ctcacgcgaa
     6481 ctcccctctc cccccccacc ctcagggtca gggtatcgtg gatcccgatg ggcagcatga
     6541 ggctggacag ctcatggtat ccgtcctccc ttttgcccag gacttccagc cagaggttga
     6601 tcttccccgg agtcctgctt gtgatcgtcc tttgatccac ggttgcggtc ccccaaagcc
     6661 ccaggtcagg gagcggaaag acggggagac tcccccccgg gcgggacccg cggtgaaagg
     6721 gttcttgggc ccgacccggt ctaggggtct catgacttgg atttttggac atatcggagg
     6781 cgcaagtcgt agaggacgga tttcaggagt cggtcttcgt tgggatccag gtttccccgg
     6841 gtcttttcct gcagcaaccc cagggtgtcg atgatttgcc gggccatggg caggtccacc
     6901 cgggtggcct tggtatccgg ctcggggatc tcccccaggt gaaccagtgc cgaagagctc
     6961 aaggaaaaga taaaagcgga cagggtgacc tcgggcaggg cggctttttc cccctgtctg
     7021 tcgtcccgag agaccttgtc gaactcgtcc tttgccctgg cgtcttcttt ttcccttccc
     7081 tcgtccccgc cacctttctt ctcttgccga tccaaatcct caccggagac cttgcgtcgg
     7141 tccttgatca caaatccctt ttcttccttt tcgtccatct tacctccccg tccctcgaat
     7201 caaagctcca accgctggac aacattgacg ttttccatgg agagcagttc ggcgaccaca
     7261 tgtttgggaa ccgacgtatc cgtgcgcagg aagatgatgt tttgcccccg ttccaggacc
     7321 tgacccacgt ccatcatgga gatgttgata ttgtggcggc ccaggcaggt accgatggcc
     7381 ccgatggtgc cgggggtatc gatgttgtaa atgagcacca ggtggccctc catggcggcc
     7441 tccaaccgaa aatcgttgat ccgaaccaat cgggggtcct ttttcccaaa catggtcccc
     7501 gccaccaggt tctggtcctc ggaggtcttg aggtgcacac tgatgaggtt ggtgaaatcc
     7561 tccgcctcgc tggtcaccat ttccctcacc ttgatgtttc tttccttgat atggagcggc
     7621 acgttcacga agttcaccat gtcccccagc acgggggtga gcatcccctt catgatggaa
     7681 aaggtgagcg gtcgggtatc catggcggtg acttcgccga tatactcgat ggaaacctcc
     7741 ttgatggctc cgcgggtgat ctgggccagc acgcttccca gtttctcgga caaaaccagg
     7801 tagggacgca ggtgtgccag cagggcgccg tccagattgg gagcgttgac ggcatttcgg
     7861 atggtccctt tgagcaggta atccatgatc tggtcggcca cggccatggc cacgttttcc
     7921 tgggcctctt ccgtggacgc ccccaggtga ggggtcacta caacctggtc gagggtgagg
     7981 agggggttgt ccgtgggagg ttccttggtg aagacatcca gggccgctcc cgctacgatt
     8041 cccgaccgaa tggcctcata gagggcctct tcgtccacca ctcctccccg ggcgcaattg
     8101 aggacgaaga ctcctttctt catctttttg aaggcttctt cgttgaggat gcctcgggtc
     8161 tcgggagtca tgggagtgtg aatggtgata taatcggagc gggcgaggag ttcatccagg
     8221 ctcactccct cgatgcccat tttgtggatc acttcggcgc tgatataggg atcgtaggca
     8281 atgaccttca tcttgagtcc cagggctcgg tcagccacaa ctcgaccgat ccgtccgatc
     8341 ccgatgacgc ccaggacctt gtggaagact tccttgccct gaaaacgctt tttttcccac
     8401 tttcccgatt tgagggagtg catcgcctgg ggaatgtttc gggacatgga aagcagcatg
     8461 gcgatggtgt gttccgccgt ggtgatgacg ttgccgtcgg gggtgttcat caccacgacg
     8521 cccctcttgg ttgccgcctc cacatccacg ttgtccaggc cgatacccgc ccgggccacc
     8581 accttgagct tctcggccgc ctcgatgacg tcggcgcgaa ccttcgtcgc gctgcgaatt
     8641 accagagcgt cgtaatcctt gatcacccgg caaagctctt catggctgag gcccgtgttc
     8701 acgtccacct ggaattcggg aacactcctg agcttttcga tgccaacctc cgccaggttg
     8761 tcgctgacta gaattctcat ctctttctcc tgaacttctc caaacgcgaa aacagcgggt
     8821 atttcgtcat ccctttccat cagggaagtc tcaactcgca acggattaca ccagcggcct
     8881 tgtctaacac cacccccgcc gcaacgtcaa gaaagaaacc ccatggtttc ctggaaaccc
     8941 ggcaacgggt ccgcccccct ttacggtttc ttcgcggcgg ccttcagttc ctcctcgatc
     9001 agcaagctga atcgctcggc agggatcgcc ccggtgtaca tcttcccatt gatgaggaag
     9061 gtgggggtcc ggtccacccc cgctttgttg gcatcttcaa tgtccttttg cacttttgcc
     9121 cggtacttcc ccccgtcaag acaagccttg aacgaaccca tcgccaatcc gatcttgccg
     9181 gcaaggagct ccagcttttc gccggagagc tcttcggtgg ttgaatacaa gaggtcttga
     9241 tattcccaga acttctcctg gtcgttggca cagtgggccg cctccgcagc ccgttccgca
     9301 tctttgtgtc gttttagggg gtaatctttg aacacccacc gcaggcggtt cccgtatttc
     9361 tccctcactt tcagcaccac ttcatgggcc tgtcgacacg caggacactg gtaatcggag
     9421 aattccacca cggcgacggg ggcatccttg ggcccccagg acggacttcc ttccacatcg
     9481 atcctggttc gaggagggga gggctccttg aggtggacga tcactccgta cttgggttcc
     9541 agggttttgg agaactccat caccccctgc aggttcttct gctgttgcag gtaggaccgg
     9601 acgcgatctc tgagctcctc ttgggttccc ttccaatccg ccatccgtcc ccggttttcc
     9661 tggagatact tctccacttc ctcgtcggta accgaagccc ccttggacag gacctcttcc
     9721 gtcaccaact gctgcaccgt gatgccccgg cttacggctt ccttgtggag cacctggtcg
     9781 gccaccaact gatccaggcg ctgtttcttc agccgataga tctcgtgctg catctcgtgg
     9841 atgcggttca ggaaaggggc ctccagttcc tgcatggtga tgacctgacc cgccacctcg
     9901 gccgccgcca cgggcgcctc tgtgcgatcg gcccccgagg ccgacgccgc ggattcgggg
     9961 ccgtgaatca gaacggaacc ggacaaaacc caaaaaaaca ggcaaaaaga aaccatctcc
    10021 cacacccgtt ttctatcgaa agagagcagt accaggagga tcacgacggc aaggttgccc
    10081 aggcagaaga cgcagatcag ctttttggaa aagaggaccc aagccaagga aagttccacg
    10141 cccatccccg cggagacaac ccagatcatc cattttttcg cccgggaaaa ggtcagcacc
    10201 aggaccccgt aaaacacgag cccccatgcc cagacaggaa gccatgccag ggtgaaagcg
    10261 gcggtttccc ggcagccgtc cgaggcggtg gtgcacagga cctcgagcca gtccgccttt
    10321 tccgacattc cggtcaacgc actcaaaaga aaccctgcaa gactcaaaat gagcagcaac
    10381 ctttctttcg cattctcctt gagcatcacc gggtcaacct cttatccatg agggtctcgg
    10441 tccaagccgc tccattgatt cgtcaccggc attgccgacc gtccaccctt tccccgactc
    10501 ggagcccgcg gccctccatc agcgcctctt ctcccgcttc ctcatcatgg cttctttctc
    10561 gtactgcttc atctccttga tgcgttcgtt gagttcgtcg atcttctgca ccgaatcctt
    10621 gagcatggcc ttggccttct cataatggaa gagggccacc ttgaaatcct gcttgctctc
    10681 atagtagcgg cccaggtggt aatgagcctc tcccatacgg ttgacctgcc ccaacaggac
    10741 ccccagtcga ttgtcgattt ccgggaaggt agcggacatg ggttcgattt tcaaaagttg
    10801 ggccacggct tcctcccggt ttccctggtc ttggagcaca agggccaact ggtagcgagc
    10861 catggagttc gaaggatcca gcttcaagca ggtctggagc acccgctggg cgtcttgaag
    10921 cctgccctgc cggaaatacg cgttgcccag agtgcacagg atgaaggggc tgccgggttc
    10981 cttacgggcg gcttccaaca gatacgacac ggcctcttcg gtccgtccca gccgcagata
    11041 gagcctcgcc aacccgtagg tctcggcgag ggttgggttg ggacgatcga accggtcccg
    11101 ggccaccttg gtatccccgt actcggcaat gagaccggcc tgcatcagct gaaagtccga
    11161 agacctgcct ttggaattct tggaggcttg cttctggggg tatttattgc acagatcggt
    11221 caggtactga atgcggtccc ccgtgtccgg gtgggtggac aggtaggaag gaatccggga
    11281 attgctgcgc aacttcacgt cgttgagccg ctgcatcacg gtcaccatgt cccggggagg
    11341 atacccggcg gagctgagat agctgagccc cagctgatcc gcttcctgct cgttttcccg
    11401 gctgtacttc aatcccaggg cctggcttcc ggcgacgctt cccatggcaa cggcgggaga
    11461 tgccgaggcg ttgcccgtgg ccgaacccag gagcaccccg gccaaaacgc ccgccagggc
    11521 ggcggcggtc agcaccttgc ctgcatccat ctgtcgatgg acgtgacgtc cctggatgtg
    11581 ggccatttca tggctgagaa tggccgccag ttccccttcg ccggccatga attcgatcag
    11641 cccccggaag acgaatatgt acccccccgg aatggcaaag gcgttgggga ccgaagaatc
    11701 caccacatag aactggtact cgtaggaagt ggtccccagc tctctcacga tccggtttcc
    11761 caccgattgc acgtagctca caatggcggg atcttccacc aggggaatgt gcgctcgaat
    11821 ctggcccagg atttttcgcc ccagttcctt ctcgtccccc agtcccaagg cccaaaccgc
    11881 gggcccgggc atcgccaggc tcaggcacag gagccagacc accaccggga ccaggaaccg
    11941 gcgtccccga accgaacgga accaacgatt catcccgaac gggtctctcg gccgccgggg
    12001 cccgttttcc tccttgtgtc ccccaccgca tccgaaccct gaaccgtttg gcgggcgcaa
    12061 cccccttttc gtcgtctcga tggacatccc ggcctcccgt cttccgaacg ctcggaagtc
    12121 atgaaggatc ggaggccgca cactgcaccg ggcccccagt gggccccgtg cgaatccttt
    12181 tggcctccga ccagagactt tccaggtcgt aaaaaggacg aacaggctca taaaagatat
    12241 gaatgatgac gtcggtgtaa tccatgagaa tccaatggcc ttcctgcctg ccttccaccc
    12301 ccaacggccg gaggccgtgc tcccgaagac gggcttccag gttctcggca atgctttgga
    12361 cctgtcgacc ggacttgccg ctgcagagga tgaaatagtc cgcaaaagaa gacagacccg
    12421 atacgtccag gaggaccagg tcctgggcca ggaagcgctc cgcttcccgg gcacagagag
    12481 cggctttctc tcgtgcgccg aggccctctc cttcccggtt cgccttagct ggcccctgtg
    12541 tgaggctcat gatcccacgc tccccttttc tccgggtttc ccggcttcca gtcggtacag
    12601 ccggttttgt tgaatatagc ccatcacggc ctcggaaacc aggtatcgaa tggagcgccc
    12661 ctgggccacc agccgtcgga tccgggtgga agacacctcg atgagggtat tgtccaatag
    12721 atacaccggc ttcagggcgg ggtcctcaaa cttgagctcc tccctgcctc ggaaataccc
    12781 cgctgaaact ttggccctca gaaaatcgcc cacggatccc tcggaatacc cccgccggtg
    12841 aagcaccacg atggatgcca atcggaacag ctcccggtaa tgccaccacg aacccatttc
    12901 gaaaaaggca tccatcccca cgagaaagaa tagctcggcg tccggggggg attgctctct
    12961 cagcctgccc agtgtgacga ccgtgtagga tttgccgggg aaatcctttt cgagagcgga
    13021 cacctggaac cgcggatttc cctggatacc cagccggagc atctcgtagc ggtattcgaa
    13081 gggaagaatc cccgttgctc ccttgtgggg cggaattgcc gcgggaatga acagcagcgt
    13141 gtccagcgcc aggaactcca cggcttcctc tgcaatccgg agatgtccca catgaaccgg
    13201 atcgaaggtg cctcccaata tccccagtct caaggctcca caggctccaa ttcgtgtttt
    13261 tcgtccctga attcctaggt ccgcacctgt ccgttcccca gggccacgaa cttggtcgtc
    13321 gtcaactcct ccacacccat ggggccaaag gcgtggagtc gggacgtgga gatgccgatt
    13381 tcggccccca atcccagttg gaacccgtca ttgaagcgcg tggaagcgtt caccaggacc
    13441 agggaagact ggacctcctg cagaaaccga tgggcccttt caaagttctc cgtaaggatg
    13501 gcttccgtgt gttgggaacc gtaccgggtg atatgccgaa tggcctcatc catccccgaa
    13561 accacgcgga ccgccaaaat caggtccagg tattccgcag accagtcttc ttccccggcg
    13621 ggcaagcagt cgcccaccag ttggcgagtg cgcgggcaac cgcggatttc caccccttcc
    13681 ttccggaggg actcgatcat gcggggcagg aatttctccg ccaccgcctc gtccaccagg
    13741 agggtttcca tggcattgca gacgccgggt cgctgcacct tggcgttcat gcacaccttt
    13801 tccgccatgt ccaggtcggc ctcccggtcc acgaaaatgt ggcatacccc cttgtagtgt
    13861 ttgaggacgg gcattcgcgc atgctcggtc acaaagcgaa tgaggtcctc cccgccccgg
    13921 gggatcatga cgtcgatgaa ttcctccaac ctgagcatct cgagcacggc ttcccggtcc
    13981 gtcgttccca ccatttgcac cgcggtggcc gggagaccct ccgcttcgag gacttcctgg
    14041 aggatgtccg ccaggcatcg attggattca aatgcttccg accctccccg caaaatcacg
    14101 gcattgccgg ccttgacgca cagagcggcc gcgtcgatgg tgacgttggg ccgggattcg
    14161 taaatgatgc cgatcacccc cagggggatt cgcatcctgc ccacccgcaa accgttgggg
    14221 cggacccaca tgcgggtcac ttcccccacg ggatcggcaa gggccgccac ttctctcaaa
    14281 ccgcccacca tttcttgaat gactttgtcc gacaggcgca gacgatccac tttggccccg
    14341 gaaagcccct ttttcagagc ctcccgaacg tccttgtcgt tggcctcgcc aatccgggaa
    14401 cggctctttt ccagggcgac agccaccgcc tccagggcct tacgtttgcg gtcgctgttc
    14461 actttggcca tctcatgggc cgcagccctc gcatctcgag ccatggtcag gatttcatca
    14521 cgcatcggat cctccctcaa agtcctcgat tccttcgctg atcacgacga aattgttgcg
    14581 gtggatcact tcgtccgagt gccggtaccc gatgatgctt tcaatttcct cgctccggtg
    14641 tcccttgatg agatcaatct ccccggactt gtagttcgta aggcccaccc cgatcacccg
    14701 gccctgcggg tccaggcaac gtaccggggc ccccaccccg aaggtcccgt gtacagcctt
    14761 gaccccaatg gggagcaggg atttcccccg ccgttggagc gccgcaacgg ccccttcgtc
    14821 cagggtcagg tttcccgccg gcttcggcag gttcgccagc cagaccttct tgccctggta
    14881 gagccgctcc cggggaagaa agagagtgcc cagaggttct ccctgaaaca agcgaaggag
    14941 caggtccctt tcccggccgg gggcaatgat catgggaatg cccgaagcca ggcacttcct
    15001 cgccgcattc aatttgctcc gcatgcctcc ggttcccacg gtcccgggcc gaggagcggc
    15061 acaggccaac agcctcgagt ccacccggtg gaccacgggg atcaggttgg cggaagggtt
    15121 gaggcgggga tcgcaatcgt agagtccctc cgtgtccgtc atgttgatca ccaggtccgc
    15181 cccgatcaga ccggcaatga gggccgatag ttgatcgttg tccccaaact tgatttcttc
    15241 gaccaccacc gtatcatttt cattgatgat gggcaatatt ctccagtcca gcagggtttc
    15301 cagggtattc ctggcattca gataccgatg gcgatgggcc aggtcctccc tggtcaggag
    15361 aacctgggcc acgtgcaggt cgtacttctc gaaggcggct tcccacgcct ccatgagggc
    15421 gccctgaccc acggcggccg ccgcctgctt ttggggaatg gttcggggac gctcggccag
    15481 ccccagtttt cggactcccg aggcgaccgc ccccgaggaa accaccacga tgcccaactc
    15541 ggggagccgt tccctcaatt ccgccatctg atcgctgagc cgatggatca tgacgcggtt
    15601 cagcccctga ggtccggtca gtactgcact tcccaccttg atgaccaggc ggcgacaccg
    15661 tccaaaaaga gccttccgat ccgtgccttc cgtcgtcacg cagactcctc tctccatgag
    15721 ttttcccgca gggcgattca gagagggttc cgacctcgca gaccggaacg gaaccaccgg
    15781 tttccgccgc ggaggacggc ccctgcccct ttcactgcgg atgccgcggg catcctcgac
    15841 tctgtgaagc acccgtccct ataatcctta gatatattgg gaggttgtac aaaatcaatc
    15901 gaaatccgga ccccggcggc gggtgcccgg accttgacgg ccgccctcag tacccccgca
    15961 accttccgat tcctcgggaa acccggccga aatccgctga gcgtcgcgga gcttcgagag
    16021 ggtttcggcc accaagcgaa ccagggcatc ggttccccgg cgctgcaggg cggagaccaa
    16081 caggacttta tggcccgtgg ttgcgtagac atcggcaatc cgttgccgct ctcgagggtc
    16141 gggcaggagg tccgtcttgt tgatcacgat aatccggagc ttttttccca tttcctccga
    16201 ataccgagac agttcgcttt ccacctgccg gtagggatca aggaggtctt cggagggaag
    16261 ggcggatccg tcaatgacgt gaaccagcag acgcgtgcgg tcgatgtggc gaagaaaacg
    16321 aattcccaga cccgttccct ggtgggctcc ccggatcagc ccggggatgt ccgcccacac
    16381 aaagggctcg aattccccac agcgaaccac tcccaagtgg gggaccaggg tggtgaaggg
    16441 ataatcggcg atcttgggcc gggcagccga gaccgctgcg atgagggtgg acttcccggc
    16501 gttgggcagt cccacgagcc cggcatccgc catgagtttg agttccagca ccaggtcccg
    16561 ttcctctccc tcccggcctt cttccgcctc ccgaggtacc tggcgaaccg aagacacaaa
    16621 tcgggcattt ccccggcccc ccgtgcctcc ccggacagcg gtccagcttt ggccgggttc
    16681 atccaaatcc accaggacct ctcccgtcac ggcgtctttg accagggtcc cgacgggaac
    16741 ctccagaacg agacttcggc cgcctctccc gtgcttgttt tggccctgcc cgtgctttcc
    16801 cgccggcgcc cgaaacaggt gccggtaatg aaagtcgagg agggtgtgct ttctccggga
    16861 ggccgtcagg aggacgcttc ctccgttgcc gccgtcgccc ccgtcgggtc ccccacgggg
    16921 gacatacttt tcccgccgga agctcacgca gccgtttccg ccctttcctg cccgtaccgt
    16981 gatcttcact tcatccacaa atttcatgac accgcgccga tccttggaaa cggaacctcc
    17041 cccgccgccc gggggatggc gaatgagccc ttcgggggcc tagaaaagac aacccgccgt
    17101 tgcaggacag cgaccggacc cgagccccgg ggtccggaat aacatcccgt ccctgccccg
    17161 gcccggcgcg attcggtgat cggaagtccc gctgccgaga agaaggggcg aaagccgccc
    17221 ccgttttctc ttcatggccg ggaatgtcgg tttgggaatg ggatggaccc gacgggcccg
    17281 cagccggaac gctccccgga ttgggaagcc gcacccggcc gcgaaagcgg ccaagcggga
    17341 cctcaaggac ctcaggactg ggtagggatg ggatagacgc tgatttgctg acgggtcttg
    17401 tctcggtatt cgaaggaaac gatgccgtca atgagcgcaa agagtgtgta atccctgccc
    17461 acgcctacgt tcttgcccgg atgaaatttc gtgcccagct ggcgcaccag aatgtttccg
    17521 gcgcgaacaa attcgcctcc gtattttttg accccccggc gttgccccgc actgtcgcgt
    17581 ccgtttcggg agctgcctcc cgccttctta tgtgccatgg atcaatcctc catcgaaatt
    17641 cttcgtgacc ggcacgaccc tgtgtcccga tttgcctcag gcctgttccg cggccccacg
    17701 ggcaacggat tcgatcaaaa gggccgtata ggcctgtcga tgtccctgcc tcttgcgata
    17761 gtcctttcgt cgcttatgct tcagaatcag gatctttttc ccccgtccct gctccacaat
    17821 gcgcccttga acggccaggc cgtccagcag gggccgaccc acctccacct ggtcttcccg
    17881 agcacacagg aggatgcgat ccagggtgac cctgtccccc acgcttccgg ggatcctttc
    17941 caccttgact accgttccca gtttggcctg gtactgcctg cccccagact gaaacactac
    18001 gtacatgaga attcctccgt gggtaagtct atcgatcttg ccccgctttc cggcccgtga
    18061 gaattcggca tcggacaaaa aaggcggccg gcacggccgc ccggggaaga tggtggagaa
    18121 ccatgatcac atggtcgcgt aaatcccctg cttcagaatc aactcctggt actcgcgcaa
    18181 tttctcgcag ttttccaagc acttgttctt gtcttctttg gctcggtcac aactcaggca
    18241 aggacttttg atcattagaa accctcctca tcccagagtg attttaaacg cctttgtata
    18301 ctggaattcg gatcatcctg tcaagaagtt tctaacgaac acgcaaacac ggttttgcct
    18361 tgcgcccagg acacgtcctc gcgtccgggg gcttgtcttc tcattcctcc cttagcatcc
    18421 cctctgggga ggggttaggg tttcagcccc cgcaatcccc gcagaaacgc ccggacattc
    18481 tgtcccagga cgctgtgatt gtatccccct tccaggacgc cgaaacagcc gatccctcgc
    18541 ttcacggccg tctcgtgcac caggtagccc atgacctggt agtcttcggt gttcagcagt
    18601 cccccccaat ccagccgatg attgtcgaac ccggccgata cgccgataat atccatctgg
    18661 gcccgttgaa gggaaagctc gacttctttc aggtacgatt cccggtcgtc gtcctcgggg
    18721 ttgtgaatgg tcacgtaccc tttgggctcg agaatgttca ccgtgccgtc tccgaaatgc
    18781 agatcgaagt ccagcacgta tgctttggtg atccttttgg cccggcgcag gtggtccagg
    18841 gcaacggcca tgttgttgaa ataacagaac ccccaggaac tgtcggcgga ggcatgatgc
    18901 cccgggggcc gaaccaaggc aaaggaaggc tcttccaggc ccagggtcgc cgcctggatg
    18961 gcggcccccg ccgccagggc ggaaatcgag tagaggcccc tgcgggtcac gttttcgata
    19021 tgccgggccg tgtgtaccgc ctcgatgtcg tcccaacccg ccggaattgc ttcctccagg
    19081 gtcacttcgt cgccgatggc atccagaatc gcttcgattc tacccggtgc cgccgccgga
    19141 tcactcacgt agacctgacg gaaatcgctg tgcgtaatga tcttcatggc gccccttcct
    19201 gtgcatcgtg aatttccttc gttgaatcga tctgttcgaa tcctccggga cgagcaaagc
    19261 cattgcatgc caaaccgtca aagagctgga gaaatgatac gggatttgtg ctgatcgaga
    19321 cggcaaccat tctcaccgcc ggggggcact cgtcggcaac gccggagact tggagcatgt
    19381 cacctcgggc acctacgccg ccgtgaatcg tcctcaaccg ccttggaagg gcgggtatac
    19441 cgccgcgtca ttgagccgag ggacccggtt ccgagctgtg ttccggcttc aagagatcaa
    19501 acaacgtctt caccgggttg aaggtaatcc gggggacctc caccaggagg gtgatccacc
    19561 ccgccatggc cccgttccac aacccaggga gctccagcgc ctcgagctca cgcccgtcct
    19621 tggatttcct ggtcaggatg acggcccggg gatccacata ctttcgcaaa tcgtacggcc
    19681 tgccctcaaa atcccggacc gcacacacca catccaccgg gttgaaatgg gtggaggatt
    19741 cccaggtcct tctttgatcc gggtcctcca ggttcacctg ggccttctcc acgatttgga
    19801 tggagagtct cccatcgctt cccttgaccc agaacggagc ccctcccggt tcccccacgt
    19861 tgcgcaccac tccgcacacc cggatggggc ggtccaaaac ctcgatcaga cggtcccttt
    19921 gctcccctgc ggaaagcttg tcaaaagccg gggaaagatc cagcaggaga tcttccgcgg
    19981 caaagcgggc ggcgctccgg atgaagtcgg cggaagggtc ctgctgcaac ccgcccaccc
    20041 actcatggac cctttcctgg acctctacaa gatagccccc caggaccatt ttccagaaaa
    20101 tccccggttc cttgaggcga tccggaacca cgttgtcgat gtttttgatg tagatcaggt
    20161 cgccttccag gtctctgagg ttttcgagca gtgccccgtg accgcccgga cgaaacacga
    20221 gtcttcccga tcgatcccga aaaggccgct tgtttcgatc gacggcgatg gtatccgtgg
    20281 aacgcttttg gaacgagaaa cccacctcat agcggacccc ttccgggacc ccgtgcaccg
    20341 actgggcctt ttcgtgcaag aaacgaaacc actcttcgtg ttcgggcgaa atggtaaagt
    20401 gcaggcggca tgaaccccgg gcatcgcgaa tgtagcgggc cgcctccacc aggtgttctt
    20461 cgaaagccgt gcgggattcc gtaggatacc gatgaaattt cagtagtccc ttgggcaggg
    20521 aaccgtaggc aagccctcgc ggggtgagaa gaaactcgag gagggatcga aactgggagc
    20581 cttggataag ggcggcaagg gtggtccctt cccggaccac ggccttctcc aggtcttcat
    20641 aaaaggcaaa ccggggcagc ccgtccagga aagcaagaaa gtcgcaggcc acggaaaccc
    20701 cctgccggac tcggcattgg agctcttccc gctccagaaa gtggggcatg tggaagattt
    20761 gcagcaggga ctgaaacatc cgagtggccg ccccggatgc gggaacaaac ttctgaaacc
    20821 tccccgcctg ggccgctatc cgctgtcttt caacggcccg ttccctttct tcggccgaga
    20881 gcaggcgaat tccgtccccg agggtgcaag ggcgcaccag gcgagagaag gaggcactcc
    20941 ggcagatcgc cagttgatca cggacctggg ccggagtgat cccctgtctc tccatctgct
    21001 tcaggtcggc tttactgaat gcatctcccc ccatacgatc acaatgacct tcctggaagg
    21061 ggttgttcca ccgggtttcc cgagcctgtt cccggctgcc gcgcgctcaa cggggcccat
    21121 ggggactact gggcaaacac ctggatattg gcatcggatt tgagctgcgt cacgtaccgg
    21181 ctcacctgct gatcggtctt ctcctgcttc aggtgttggc cgatcttctc cttggtttcc
    21241 tccagggacg ccacggacgc cggtttcttg cccgtcacct gaataacgtg aaacccgaat
    21301 tgggtttcca cgatttcgct gatttcgcct tcccggagag aaaaagccgc ggcttcgaac
    21361 tggggcacca tctgaccccg tttgaagaag tccaggtccc caccccgggc gccgctgggg
    21421 cagtcggaag tctccttggc cacggcggca aaatcctcgc ctttcttgat ccttgcggcg
    21481 atctgctcca tcttcccgcg ggcctgggct ttgtccgcat cactgccctt gggctccacc
    21541 ttcaccagga tgtgacgggc ccgcaccatc tcgggagact tgaacaagtc cggattgctg
    21601 tcgtagaagg ccttgatttc cggttcggac accgaaacct ttgcggcgac cttcaggtcg
    21661 atcagtcgct ggacggaaat ctcacgccca aactgcgccc gcagatcctg ctcgctcagg
    21721 ttgagccgct gaagggccgc ccggtattct ttgtcaccgg gaaaccgggc cttcagcgca
    21781 ttcacctgtt cgtcgatctc ggcctcttcc gtctggatgc cctgtttttg actgtcttga
    21841 tagagcaatt cccgatcgat gagattttga aggacctggt tcttgatttc aggcatctgg
    21901 cccacgctcg ccggatgtcc catggccacc atctggtttt ccaccctttt caaggcagca
    21961 tcgagttcat cctggctgat caccacgtcg ttcaccttcg ccgcttcttt cttggctgca
    22021 ctcttttcct gggccactcc ttcacctcct gaaatcaaca gcatccctaa agcgatcccg
    22081 aacaaacacc catatgtcca actccatctt ttgtgtacca tacgttcatt ctcctctggg
    22141 ggaaaaaaac aacccacaaa ggcttgtgat tccttcgtgg gttcgggcgc atccgtgggc
    22201 gccgttccaa acttggtatg caccggagtc tcttgtcatg ggcgatttat aatgggttgc
    22261 cgtgcaaaag tcaaaggaag cgcggtccga aacccgcccc ttcaggcccg ggtcccgaac
    22321 tcaccctggg caggccttcc ggaccctcga aagacgagcc ccccggcccg gctccgtctc
    22381 ccgccaggga agtcccgggg acgctcaggc aggcttcttc ctggggcggg cggcccctcc
    22441 ccctgcccaa tcctggtaaa agatgagcag cgggctggcc acaaacaccg acgaataggt
    22501 ccccaccacg atgccgatga tcatggcgaa tgcaaaatcg tgaatgaccg tacccccgaa
    22561 caggaacagg aacaccaggg ccagcagggt ggtccccccc gtgagaatgg tccggctcag
    22621 ggtttgattg atgctgtcgt tgatgagctt cttgaactcg tctttccgga ccttgcggac
    22681 gttttcccgg atgcggtcga agaccacgat ggtgtcgttg agggaatacc cgacaatggt
    22741 caggagggcc gccaggatct ccagggtaaa ttccttgttg gtgacggcaa agatcccgac
    22801 ggtgatgaga acgtcatgga ccagggccag caccgcggca agggcatagg gaagtttgag
    22861 gacccagcag aatcccatgg tgaccaccag ggcccccagg atgaggtaag acaccgatat
    22921 ccaaatggct tccaggaggt agacgccgat gatgaggacc ccgaccatca tgccgctggt
    22981 ggcccacttg agctcgaacc gtccggacac gtatacggcg atgaagagca gggcgtaata
    23041 gatggcgaac agggccttct gtcgcaggtc gtggcccacc ttcggcccca ccatttccgc
    23101 ccggcgaacc gtcgcctgtc cctgcccgta tgccttcccc agactctcct ccactcttcg
    23161 ggagaggtcc gccagctcgg acgtggtcag gtcggtccgg atcaggtatt cgttatcgga
    23221 ggcggccccc acctgttgaa tggcgctctg ggcgaacccc aaatccgaca gggcgtttcg
    23281 aatctcgtcc gggctggttg accgctggaa ctgaatctgc accagggtcc ctcccgcgaa
    23341 atcgaccccc aggttcagac cgccccgcca aaccatggcg gccaggctga ggagaatgag
    23401 gaggctcgaa agcacgaacg ccttgaaacg cattcccacg aaattgatgt cgatgtccgg
    23461 cctaatccat tgcatgatgg cgactcgttt tccttttcac cgttaaatgc tgacagtacg
    23521 cacccgccgc tggagcaaaa ggtagtcgaa gatgatcctg gtcacgacga tggcggtgaa
    23581 catactcgtg acaatcccga tggtcagtgt gaccgcaaag cccctcacgg gtcccgtacc
    23641 aaattgaaat agaacgacgg cggcaatgag ggtcgtgatg ttcgagtcaa aaatcgtcaa
    23701 ggtggcccgg gaatacccgg tctcgagggc cgcccgcaag gtttttccca gcctcagctc
    23761 ctcccggatt cgctcgaaga tgagcacgtt ggcgtccacg gccattccca cggacaggat
    23821 gatgcccgca atgccgggca aggtcagggt cgctccgaag gccgccaggc aggccataat
    23881 caaggggatg ttcaggatca gcgccaggtc cgcaatgaga cccgatgcac gatagtagac
    23941 caccatgaaa acgatggtca caagcccgcc caggacggat gcgagcaagc cctttcgaat
    24001 ggagtcctgt cccagggagg gccccacggt gcgctgttcg aggatcttta ccggggcggg
    24061 caaagctccg gcccgcaaaa cgatggccag gtcccgggct tcttccatgg tgaaggaccc
    24121 cgtaatgctg gctttcccgc cgctgatttt gtcctggatc accggggccg aatacacggt
    24181 cccgtctaag acaatggcca ggcgtttctt gatgttttct cccgtgaccc tctcgaatat
    24241 ccgggcccct tccgaatcga actcgaggcc cacgtaaggg gtgctgtact ggctgtccag
    24301 ccgcacctcg gcggtggtga gcgcctctcc cgtcaaaacc gtccggtctt tcagggcgat
    24361 ctgggtcatg tccaccgcac ccgtcttgct gtccacgtgt ttcatgggaa agactttgac
    24421 ccccgccggg gtctttccct ccttgacgtc ctggggtgtt acgtcttcgg ctaccaactt
    24481 gaactcgagg agggccgtct ttccaatcag ggcaacagcg cgttgggggt ccttgacccc
    24541 gggaagctga atgagaatcc ggtcctctcc ctggggacga atgtccggtt cggtgacccc
    24601 aaactggtcc acccggtttc gaatggtttc cagggcctgg ctcgaagaca tctttcggat
    24661 atggttcact tgaaagtcct ggagggtcag ggtcacctgc atggctccct cggcaacgcc
    24721 ttccgccccc accaacttca tgacgggata gtccttttcc accaacttgg cgagaggggt
    24781 ctgggcctcg gcgccggcca gttggacgac gacatcccac ttcttggtcc gctccacctt
    24841 ggtgaaggga atcctctcct tgcgaaaggt ttccttcatc tcctccgcga cccggtccgc
    24901 gtagttctcg acggctttat ccgtatccac ttcgaggatg agatgcatgc ccccctgaag
    24961 gtccaatccc aggtggatct tttccttggg gaggaccttg ctccaccatg gaggaagggt
    25021 cgaagtgaac gtgggtgtca ggtagaccag ccctcccagc agaaccacca caaccaggac
    25081 aactcgccat ttcaggttct tcaaatccgg acctccctgt gcttggcagg attttcaaca
    25141 aaggccgaca ggaaaaaccc ccgccgcccc cgggcggggc aacgaaggtt ctgggggtga
    25201 aagcagcccg ggcgggctat tccgcaacct ctcccttgga aacaagccct acgatgtaag
    25261 ggcgctggat tttcactctc accttgtccg cgatctcgag ggtcaccacc gtatcggtca
    25321 gccccgtgat ccttccgaga atccctccct gagtgatcac cacatccccc ttctggagac
    25381 cgctcagcat cgctttgtgt tctttctgtt tcttttgctg ggggcgaatg aggagaaaat
    25441 agaagatgac caccataaaa atcagaggag cgaaggccgc aaagcctcct gcagcaccct
    25501 gccccccgtc gccttgaccc atcgagccca tcgcgtgcgc cacaccgacc caatccatgc
    25561 gttcctcctg aaaggaaggg gtttcatccc ttccgcaatg gtccgaaacc cgctccccgg
    25621 gcttccgtgt ctctatccgt ccacccccgc tcgaagactc cggggggatc gcaaaccgcc
    25681 cctgtgtccc tcgtgcgaag acgagagtcc cggcacgtgg ctgcggagcg tctcaccggc
    25741 gggaaggctt tgccaaagcc gttcgccctt cccgaaatcc cggccctccc aaaaacgaag
    25801 tgacaattta aacgatattt tagcgagatt gtcaacgctc gaaaaccgaa ggccctcccg
    25861 gcgggagtcc gtcgcccacg gatatccgct cgcctgcctt taccttttcg aaacattccg
    25921 ttatgttcct catttgagtg agcgattccg agagcatcag cccgtcatgc ccgccaaggg
    25981 gggcatccag tcggtatgca ctccctttcc ggatccccgc tcgcgcggaa atcacgaaac
    26041 gggattcgga ccggccaaat cgttccatcc gggatgtagt tacgagccac ggatcttcga
    26101 gggggttccc gccacccgaa cgatcatccc ggcgaccttg tatgcgaaga cttcccgaag
    26161 cattcgttcc gacggaacgt caacgattgt cgattcctcg agacaccacc acagcggatc
    26221 aagtcatgaa cgaccatgtg gatcttgcaa ggctccaaag cgaaatccga cacaattgcg
    26281 acatttccga cgccaaccac gcagggctct tctccatctg cggcctcctt cttcgcttga
    26341 gggacctcta caagtgggag cattccactc caccctggca agaaccggaa ccggccgatc
    26401 tcatggagtg gatcgatgcc cgggaaaccc gttgggaaga ggttcttcat cgtccctggg
    26461 cttctcttac cgtgggagac gaaacgtttc agccgttcga aatcgcctcc atcaacgcga
    26521 ggctggcccc ccacggcctg atctacggtg cggggtacgc agtgggaatg aaaccgtcct
    26581 tcttcctggg cgaaatcctc gaaacccggc ggctgggctc cctgactatc cacgtggtgg
    26641 gaaaagaatg gatccgggac ctctatgcag cccccgccat gaggcaaggg gagcaaatct
    26701 ttgcccgccg gacccccatg cttttcttcc tgtgggacca ggtcatggaa atgcgccctt
    26761 cggcccggga acccctctcc tatgcctttg cccagtacgg cctcaacgtc gattccctgc
    26821 ggcgtcttcc cgccgagatg gggccccggc tcgagcccgt ggccgcatcc gaattggaga
    26881 cgtggattta tcacgaagtc ggggaagcca gcgacacgac ctttcaaggc ctgcaatggc
    26941 aggaaatcgt ctcctcgtac gccaacagcc ccgtggaaat cttcacacgg gtgatcaaag
    27001 acctcctttc ggacacgcat ccccaagggc tgctgggtca catcatccgg cacgggatca
    27061 aaccctccct ggccttttac gtatcgttca tgcgcccctt cacccgggtg ttgtttccgg
    27121 agatccgcgt cgcttttggc gactttatcc ggcgcgacga ctggacggga atcgagcggg
    27181 cccgggccgc cgggtatgaa aaggcaaggc acaacggctt ggagctgatc cgcctccatg
    27241 aggccggcaa ggggcatgat tcccagcgga cccgggagaa gatcatctcc catctcatcg
    27301 agcctctagg ggtcctgggc accctgactt cccgggagga cgaaccctag gtcccggaac
    27361 ccggacctta tgggggatcg ccatgaaaac atgggttgcc gaccttctca tctgccccgc
    27421 ctgcctgcct cgcgagcacg ggctcatcct gcggaccgag gagcgcgctc gagacgaaat
    27481 cctgctggga tccctggact gtcccggctg tgcttccaga taccccatca ccgacgggat
    27541 cgccgatctg ctcccggcct ctttcgggtc caacggcgcc atcgggggga agtacgaaca
    27601 gtccccggtg gtttcgtcct acctctggag tcattacgcg gatcttttcg gcgatccgga
    27661 tgtgctgaaa agttatgagc aatggggggc cctggtggaa cccgtcccgg ggttgtccct
    27721 ggatatgggc tgcgccaccg gtcggtttgc cttcgagttg gcctctcgat cggcgggtgt
    27781 cgtcggcatc gattcctccc ggaacctggt ccgggcggcc cgggaactca tgatccaccg
    27841 gagactgcga tttgccctcc gcctggaagg gcaaatccgt cgggttcaaa ccattgaagt
    27901 cccctcgtcc tgggattgcg attccatcga ttttctccgg gccgacgcct gcaatccacc
    27961 cttcaggacc gggactttcc acggcgtcgc ctccctgaat ctgctggaca aagttccccg
    28021 tcccctgaga catttacagg aagcggaccg ggtcgcggga aaacaggacg ctcagctcct
    28081 ggtatcggac ccgttctcct ggtccgagga ggttgccgac cccaaggact ggctcggggg
    28141 gaaaacccgc ggcaagtatt cggggagcgg gtgggagaac ctag
//
LOCUS       DAOOVC010000042        28006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1153_length_28006_cov_6.728060, whole genome shotgun sequence.
ACCESSION   DAOOVC010000042 DAOOVC010000000
VERSION     DAOOVC010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 28006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28006
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1153_length_28006_cov_6.728060"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="PLM79_07620"
     CDS             <1..740
                     /locus_tag="PLM79_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV86216.1"
                     /translation="TSDSFSETCMMMGTRYRFPIIMHVSENESEVEQIRGRYGLRPVA
                     HLDRMGLLGPDFVALHCVALDDQDMDLLAQNKVHVVHNPESNMKLASGIAPVPRLLDR
                     GVNVALGTDGCASNNDLDLFAEMDTCAKLHKVSRMDPTVLPARSVLGMAVGSGARALG
                     FGADLGEISTGKLADIIVLDFNRPHLTPLYDTTSHLVYAARGADVRHSIIHGRLVMED
                     RQVLTLDVDEVMDHVRNLARAVRTTPG"
     gene            697..2091
                     /locus_tag="PLM79_07625"
     CDS             697..2091
                     /locus_tag="PLM79_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOV86217.1"
                     /translation="MSEILPEPCAPPRDDAVCREVDLLLLNADWVVTCTQEAGCIPRG
                     AAAVRGDTLEALGPTAVLRRQFRGRREINLEGHVLIPGLINTHCHGAMSCLRGLEDDL
                     PLDRWLHEVIFPVEAAVMTEETVYWGTLLSAVEMIRNGITTVCDGYFFEEAATRAILD
                     SGMRGVLGQGILDFPTPDCPDPAQSRRRAESFLDSFPTEHPRVRPSLFCHAPYTCSSQ
                     TFGWAKALCKERGILFQTHLSETASEVRDLTELHGERPVFYLDRLGILDEQTLCVHGV
                     WLSSPEIDLLARRGAGISHNPESNMKLASGVCPLTEFLSRGARVGLGTDGCAGNNDLD
                     LFSEMDRAAKLEKVFRADPTSGAAVRIFELATASGARVLGWEDELGSLRPGKKADLVA
                     VDFRQPHLTPVYDPISHLVYAVRGSDVRHVWVNGRQVLADRHLVGVDEARVMEEVRKV
                     AEQVRHIRSGGPRA"
     gene            2235..3614
                     /locus_tag="PLM79_07630"
     CDS             2235..3614
                     /locus_tag="PLM79_07630"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86218.1"
                     /translation="MREKHKILIVDDEAPFRSALCRLLEKQYIVVEAKTGEKALELAY
                     REEPDLVLLDIGLPDVSGLDILPRFKELRPSPTVVMITAYEHVKDIVAAIRLGAFDYL
                     VKPVDLDEFEMTVQKALEHAGLKNEVDRLRQEVQRLQKVDRLIGRNPKFLDAQVLAIK
                     SAQSLDAGVLIQGQSGVGKELFARLIHAKSPRAAYPFVALNCAAFSSEIIESELFGYE
                     KGAFTGARAEGKQGLLEVANGGTLLLDEVVELHPEIQAKLLRVLEEKEFYPLGGTRRR
                     TVDIRIVSACNQDLWQAAEGGRFRKDLYFRLATIRLDLPSLRERPEDILPLAQFFMEE
                     FNDKYGKHFRRLSPEAELILLTHPWQGNVRELRNTIERVVLLENDETILGRYLNFLGP
                     QSGEPPAGTGGLRIELPEDGIALEQVEKHIILQAYEKCHRNKSKTARYLALPRHILIY
                     RLKKYGIED"
     gene            3870..4241
                     /locus_tag="PLM79_07635"
     CDS             3870..4241
                     /locus_tag="PLM79_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86219.1"
                     /translation="MDFQIRVLLVEDDPPLGRSLINFLERAGYVFESCSTACEAIALA
                     QQHRHDIVISEFHLPDANGVRLFEQLKRVVPNAKAILISEFDFQAVASEVGQVEVHAF
                     LKKPFDLAELETALSFSHAYS"
     gene            4753..4929
                     /locus_tag="PLM79_07640"
     CDS             4753..4929
                     /locus_tag="PLM79_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86220.1"
                     /translation="MVAQKLELIQKMEQKGMTVAQAAEAMGFDAQLLGLYLVKDAYPV
                     PKRILDKLAEVISN"
     gene            5619..6566
                     /locus_tag="PLM79_07645"
     CDS             5619..6566
                     /locus_tag="PLM79_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOV86221.1"
                     /translation="MKQEKVFAQLEQKGVSRRDFLKFCSFVSATMGLAPSMTPKVAEA
                     LTAPQRPPVVWLSFSECTGCPESLLRTTYPWIDELLLEIISLDYSETVMAASGQAAED
                     ALHATVKKHPGKFICVVDGAIPTAENGVYGMINGKTMIEIAKEICPKALANICIGTCS
                     SFGGVAAAKPNPTGAKDLESALGPALTTPVVKIPGCPPNGINLVATIVNFLLFGKLPD
                     LDAKKRPLFAYGKTIHDTCPRRSHYEMGEFVTEFGSKEAALGYCLFQLGCKGPETYNN
                     CAIVKWNDGTSWPVQAGHPCIGCSEPDFWDKMSPFYKSL"
     gene            6646..8283
                     /locus_tag="PLM79_07650"
     CDS             6646..8283
                     /locus_tag="PLM79_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOV86222.1"
                     /translation="MAQRIVVDPITRIEGHLRIEVEVGDGKVKNAWSSSTLFRGLEII
                     LQGRDPRDAWLFTQRACGVCTYVHALASVRCVDNAVGVTVPDNARLIRNLLMGAQFLH
                     DHIVHFYHLHALDWVDVVSSLKADPKKAAALADQVSPAGGNVNEFKAVKARVKKFVDS
                     GQLGLFNNGYWGHPAYKLPPEVNLIAVKHYLDALRQQARTARMHAVFGAKNPHLQSLV
                     VGGVTCVADLNADRIAEFKYLWKETMDFVNNVYIPDVVAVAGFYKDWGKIGGTTNFLA
                     YGEFPQSAKEPESLLFPRGAVFKRKISNVQPVDINLIEEHVKRSWYEGDKALNPAAGE
                     TKPKYESLDVNNRYSWMKAPRYNGEPMEVGPLARVLIAYGKGHAPTKKAVDGLLKALK
                     VPQEALFSTLGRTAARALETKIIGEAMEGWMGQLVENLKKGDTKTYQPYEMPAQASGV
                     GLNDVPRGALGHWIEIKDQKIGNYQLVVPSTWNLGPRCAGDKPGPVEEALMGTPVADP
                     KRPVEILRTVHSFDPCIACGVHVIDPQTNEVYKFKVV"
     gene            8385..8894
                     /locus_tag="PLM79_07655"
     CDS             8385..8894
                     /locus_tag="PLM79_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HOV86223.1"
                     /translation="MSEKQILVLGVGNLLLQDEGVGVRVIETLEAGYAFSSNVTLLDG
                     GTLGLRLLDPISRADHLIVVDAVQNGEAPGTLYFLDGKNLFNRVTFKNSMHQLDLREA
                     LAYAEVLGNSPTAVVIGIEPGDISPWGLELTESVQSRLEDMAGLVLKEIEKAGGTYVA
                     KNSPGAALQ"
     gene            8910..9155
                     /locus_tag="PLM79_07660"
     CDS             8910..9155
                     /locus_tag="PLM79_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOV86224.1"
                     /translation="MCLAIPAEIVQIDDETATVRVGEALRKASLMLLPEGADLGDYVI
                     VHAGFALHKVDPEEAMESLKLLRELASFGEEESPLGT"
     gene            complement(9878..10195)
                     /locus_tag="PLM79_07665"
     CDS             complement(9878..10195)
                     /locus_tag="PLM79_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86225.1"
                     /translation="MTGIFFAGDDVRVFDHFVVVSKRQFGPWVGDGRSLGVCGPHKEK
                     GLTPIGGNALPGRILPVIRRFRQLPRLPIVLGGAFPGNGLRIGNPLGPGFQPQGFLDG
                     WGL"
     gene            complement(11048..11563)
                     /locus_tag="PLM79_07670"
     CDS             complement(11048..11563)
                     /locus_tag="PLM79_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86226.1"
                     /translation="MASGGRKGLILMILACVLLVGLGGLAVVLNSPPVPKEFDRFRGI
                     PWGASVGEVPGVKLLAEDGDLTFYVRDGDQLSFEDVEVNNIVYGFHKGHFYNVIIYYR
                     TADAFSKIKERFYQQLGNAYHPVQSEKRYFWDGDKVGVLLTFDDPSDEGRVSFLFKPV
                     ANEVVAKEGRP"
     gene            complement(11672..13177)
                     /gene="asnS"
                     /locus_tag="PLM79_07675"
     CDS             complement(11672..13177)
                     /gene="asnS"
                     /locus_tag="PLM79_07675"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOV86227.1"
                     /translation="MGWIAAPEPGGVLRPGWAVIEPNPEIARKGPCAMEKGICIADLF
                     RRENEYLGKPVLVQGWVRTRRDSKAGVTFIELNDGSCFRNLQVVAETALGEHKPTMGS
                     VTTGSSLRVEGTVLASPGREQSIELRAHRIQVYGGADPAKYPLQKKRHSFEFLRQIAH
                     LRPRTNTIGAVTRVRNRLSQAIHRFFQDRGFYWIHTPVITSSDCEGAGEVFRVTTLDL
                     QKAAAALREGGNPFEEDFFGKPVYLTVSGQLQAEIYALSLGKVYTFGPTFRAENSNTS
                     RHLAEFWMVEPEMAFYDLEDDLELAEAFLKSLAQAVLADCEEDLRFFARFVEPGVMES
                     LDKLTREPFQVMTYGEAVEVLRKSGEPFAFPVSWGSDLQAEHERYLAEKYVGGPLAVV
                     HFPRDLKPFYMRRNDDGKTVAAMDILVPRVGEIIGGSQREERPDVLLDQMKQKGISVE
                     DYRWYLDLREYGSAPHAGFGLGLERMVQFVTGVANIREAIPFPRSPGSADF"
     gene            13390..14715
                     /locus_tag="PLM79_07680"
     CDS             13390..14715
                     /locus_tag="PLM79_07680"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOV86228.1"
                     /translation="MSRQFYQTLLEQIVTWCLNAGGWLLLPAVCYGLRNHGEFLKGRL
                     GNFSLDAADRKGPRIWFHAASVGEVNGAVPILHLTHRHLPRAFICLSVGTPQGHEFAK
                     SQLPAWATVVPFPVDVPWIVDKSLKRIRPDLYVGFESEFWPVLLGRLRRAGVPALLLN
                     GRLSRRSAEKYRFLRPVFQPLFQHFRRIALHSEQDRQNLLDMGVSPDRIRVVGSSKHD
                     GLRLRVRPEAAARWRGLLGIPENTPVLLGGSLRRSECTGLLEVFCELRKTTDSCLGIF
                     APRHLHQVLPMAEWLTNRGVPFQRLSEIEGRRTRREAPVVLVDRIGVLFELYALGDLI
                     FCGGTLEPIGGHNILEPAAWARPVFYGPHVEKVREEHRVLAKKKGGFPVRDFDDLRQQ
                     WAYWLRHLPELKSFGENAKDALDFLGGAAEKQVELLLQTLSESGARVGA"
     gene            14712..15500
                     /gene="larB"
                     /locus_tag="PLM79_07685"
     CDS             14712..15500
                     /gene="larB"
                     /locus_tag="PLM79_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HOV86229.1"
                     /translation="MTTDRLQTLLQEFKDGKKTLEEVLDFLKRLPFEDLSFARVDHHR
                     GLRRGFPEVVYGEGKTARQIIDIVGAMQRFGSDILVTRVEEGKAREVASTVPGMTYHP
                     LAGILSFCQEPHGEKAPGSIRVLCAGSSDIPVAEEAAVTAEFMGNRVGRHYDVGVAGL
                     HRLLQIWDEIRDASVYVVAAGMEGALPSVVAGLVDRPVIAVPTSVGYGAGFGGIAALL
                     GMLNSCAPGIAVVNIDNGFGAGYLAGLINRRSGAGQARAESETA"
     gene            15594..16019
                     /locus_tag="PLM79_07690"
     CDS             15594..16019
                     /locus_tag="PLM79_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86230.1"
                     /translation="MAREYRPNVRESAVISRLDHAKESQRINALNLLRQHLEEVSRRI
                     VTKLVEERLVETTSMDDLEGQIQQALQTMLTSEEFEIQYQTANLRTLVPRPHFISLYV
                     TAFIVEKLIDHPSVKEIFGTDEDIYACVNRQVSRFIPLQ"
     gene            complement(16084..17511)
                     /locus_tag="PLM79_07695"
     CDS             complement(16084..17511)
                     /locus_tag="PLM79_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOV86231.1"
                     /translation="MPRTPFVLLVNPWITDFAAYDLWSKPLGLLTLASLLRENGIQVA
                     LVDCLDRYDPFSNTHPDMKPGVSRSYGTGKYPKMKIEKPSPYRDFPRFYYRHGIHPES
                     FRQKLRALPKPDLIWVTSMMTYWYPGVQQTIGALRDVFAEIPIWLGGVYARLCEDHAR
                     RTSGADLVVTDPLPRIGRRVEDTLQVPVRNKDRWGDLSFLPSPAWDLVPLLEYVPILA
                     GRGCPFRCPYCASARLEPRWERRSGEDIHREISRWHGERGVVDFAFYDDALLLHAESS
                     LRPALERLCRDGVALRFHTPNAVHVRALTPEWCDLLFAGGFTTLRLGLETTQPQRHRQ
                     WGGKVEADMFEEAVRNLARAGFSKDRIGVYLLCGAPGQSPREVADAVNAVRREGLSPF
                     LAEYSPVPGSPMWKDAVQVSSFDIENEPLYHNNSFFACRRADFSYRDLLELKDLALQA
                     RRAVSGAENAPGGDKKKTHGPGMGP"
     gene            17733..18182
                     /gene="dtd"
                     /locus_tag="PLM79_07700"
     CDS             17733..18182
                     /gene="dtd"
                     /locus_tag="PLM79_07700"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOV86232.1"
                     /translation="MRAVVQRVSRARVEIRGETVGRVDQGLVVFLGVGPDDGPEDARS
                     MADKVLHLRIFPDGEDKMNRSVLDLRGQLLVVSQFTLYGDCRKGRRPSYAGAAPPEKA
                     RLLYEAFVSELRGSGLGVETGQFQEQMEVHLVNDGPVTLLLDSKKKF"
     gene            complement(18319..18726)
                     /locus_tag="PLM79_07705"
     CDS             complement(18319..18726)
                     /locus_tag="PLM79_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86233.1"
                     /translation="MDLLFFSPAQDETHRILDGLLRNIIHRERLLTCSSIEQLRRAMI
                     HGSYDLLAGVFFPRTQLDLSDLLKMREHFRDLPIVLILPDSDADTVARGHGLRPRFLT
                     YADADFHELMAVVTRILLRRATHHYPLTEFAGR"
     gene            complement(19121..20527)
                     /locus_tag="PLM79_07710"
     CDS             complement(19121..20527)
                     /locus_tag="PLM79_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86234.1"
                     /translation="MANVLIIDDDPGICRLLSGVVKKMGHEVAYAHTLAQGISAAHAG
                     EYDVVFLDVGLPDGSGLDGLPEISETASRPEVIIITGAGRLSGAELAIKSGAWDYIQK
                     PSTIQEMTLPLVRALQYRQEKRDRSPGSRLMALDRCGIVGESPRIKTCLDLVAQVAGT
                     DATVLITGETGTGKELFASAIHRNSPRGSRPFVVVDCAALPETIVESMLFGHEKGAFT
                     GADRTREGLIKQAHGGTLFLDEVGELPLQLQKAFLRVLQERRFRPIGSKSEVESDFRL
                     VSGTNRDLQKMVEEGSFRSDLLFRIRSFTIELPPLCERPSDIKDLAMHHLANLCERYR
                     LGLKGFSPDFLEALLAYRWPGNVRELFHALERALAVARDEPTLFPIHLPDSIRIHLAA
                     MLLSKKTAPGPPRAETSLRSESILPFKEFRETMDRQYLTHLVGHTGRNIKEACRISGL
                     SRSRLYELLKEYNIADPA"
     gene            complement(20770..21267)
                     /locus_tag="PLM79_07715"
     CDS             complement(20770..21267)
                     /locus_tag="PLM79_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV86235.1"
                     /translation="MSTKTEKNLKEAFAGESQANRRYLAFAERAEDESLHGVAKLFRA
                     VAAAETVHALKHFRVAGGVGKTRENLQEALSGEMHEFKNMYPQMISDAKEEGQKAAEI
                     SFHYANEVEKVHAALYAKALEDPEKFPVKDYHVCKICGYTVGDQAPDKCPVCGANKKA
                     FFKVG"
     gene            21857..>22218
                     /locus_tag="PLM79_07720"
     CDS             21857..>22218
                     /locus_tag="PLM79_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOV86236.1"
                     /translation="MSTPFQPGMTHELKIKSTPEHSARVFYPHLPDVFATPFLGGLME
                     RVSAELINERLQPGEQSVGISMDLKHTAATPLGMEIRVVTKVTSVEGRKLTFRLEAFD
                     EVEKIGEATHERFIIQAD"
     assembly_gap    22219..22318
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22319..24091
                     /locus_tag="PLM79_07725"
     CDS             <22319..24091
                     /locus_tag="PLM79_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOV86237.1"
                     /translation="RLVSQAIHDCGEKGVKGAVVISGGFSEAGPEGEALQAELADTAR
                     KTGVRVLGPNCQGVNNPYGPLCASWPLLTRRGRVAVISQSGTVGAAMMDWFSVEDLGV
                     SGFVSLGNRADVDEIDLIEYYEHDPHTRVIAAYLEGIKDPARFQKVIQSLKKPLVVLK
                     SGRTPRGKVAAESHTKSLAGADSVYSALFRRYGIYRADTIEEFYDISKALAYLEPPKG
                     NRVLFITTSGGAAILATDAAEQEGIEIPPLPEELSTQLEGVIPPHAIRANPLDLTGDA
                     NAKMFREVIQRARPYYDTLGILFGDPIVDASQVVSAGANELVVFLGGAEVERAEKLKM
                     HQMGIPVYPTPERGMKALASVIPPEKKTGRAAFTVPVARGRTQLSFFESIKFLESKGF
                     GCIVSRFADSPGKAVHVAHQLGLPVSLRIDSPDILHKSDYGGVRLNLLSAQDVRAAYT
                     KMMEEVKTRFPDARINGVVVSAMATPGLELILGMNRDPQFGPILVFGVGGTAVELYRD
                     VSMRLLPLNREEVLQMMSEIKGAPLLRGFRGRRPVNENAVADAILKLAQVAEENPDIV
                     EIDLNPVFAYSEDIVVVDARILKA"
     gene            24161..24643
                     /locus_tag="PLM79_07730"
     CDS             24161..24643
                     /locus_tag="PLM79_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOV86238.1"
                     /translation="MQVLVLYYSRGGNTRKLAEAVVQGVQTVPSVRAVLRTTEEVTKE
                     DLLDSAGIIAGSPVYFGVMAADLKRVFDEHVGIRKKMENKVGAAFTTSGDPTGGKETT
                     MMSILQCLLIYGMIIVGDPLSATGHYGVACTGPPDESALENGRKLGQRVAELCLKLSS
                     "
     gene            complement(24816..25454)
                     /locus_tag="PLM79_07735"
     CDS             complement(24816..25454)
                     /locus_tag="PLM79_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOV86239.1"
                     /translation="MNSRMHLREEVVWVCRRLAEKGLVAASDGNVSCRLDSNYVLITP
                     GGRHKGDLECADLLVLDETGMPVEGPGTPSSELRMHLCVYENRPDVEAVVHAHPPMLT
                     ALTLAGLPFCAELLPEVWLTIGPVPTAPYATPSTREVPDAVRGLIGGHEALLLERHGS
                     LTVGRTVREACMRLEKLEHAAHIQFYAHLLKNGPPDPLRTEDLLKLQALKKQ"
     gene            25621..26772
                     /gene="ftsY"
                     /locus_tag="PLM79_07740"
     CDS             25621..26772
                     /gene="ftsY"
                     /locus_tag="PLM79_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOV86240.1"
                     /translation="MIRWFRRKGKTVPAAQGPGLQEPGGSTLETEESPSEQAAQPQKP
                     QVEVQGKEVVDEDLASSPDLVQEEADLDETEGPRDEKVSFFRRLRDRLHKTRTQLVNR
                     VDRLVFGKKVIDLELLDELEEVLITSDLGVHTTQTLLGAITERIQRRELSDPQKLRGQ
                     LREEIQRILSVEAPPLDLGKTRPFVINVVGVNGVGKTTTIGKLAHQFKARGLSVMLVA
                     ADTFRAAAIEQLSLWGDRVGVPVIKQKSGSDPSAVAFDAMEAAVSREVDVVLLDTAGR
                     MHTKVNLMEELKKVQRIVARKMPGAPHETWLVLDAVTGQNALSQARLFKQEIGATGLI
                     LTKLDGTAKGGIIVAICNELQLPVRYIGIGEGMDDLRSFDPEEFTRALF"
     gene            26783..27955
                     /locus_tag="PLM79_07745"
     CDS             26783..27955
                     /locus_tag="PLM79_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOV86241.1"
                     /translation="MDGRLGGIRVLDLSRLLPGPFCSMVPADLGADVIKVEDPKLGDY
                     IRWRPPLIGRNSAFHVVLNRKKRSLTLKSPEGKELFRRQEMKADVLLKGFRPGGMRRL
                     GLGYESLKTLNPLPVYCAITGYGSDGPLASRIRHDINHLARSGVFSYSGRDGIPTLSG
                     VQIADLGGAGLPAAFSIPAAMLARLKVGEGQLVDISRTDGALAWNCLRWGKTLADGVV
                     PCPGDDFLNHGFACYNLYRTPDDRTMALGALEPQFWEAFCSTVDRPEWKRPDDTEPGP
                     HQAVLIMEISAMFARKSSREWVEIFSKADCCCELVLNLREVMDDPQVRARRMVVEVVH
                     QSWRAYRQRGIAPKFSATPGSIRTCSPERGEHTDSILAEMGLETHEIGALRSGGVV"
BASE COUNT         5783 a         8357 c         8292 g         5474 t
ORIGIN      
        1 ccacctccga ttcgttttcg gaaacgtgca tgatgatggg gacccggtac cgatttccca
       61 tcatcatgca tgtttccgaa aacgaatcgg aggtggaaca aattcgcggc cgatacgggc
      121 ttcggcccgt cgcccacctg gaccgcatgg gtcttctggg acccgatttt gttgccctcc
      181 attgcgtggc cctggacgac caggacatgg acctcctggc acagaacaag gtccacgtgg
      241 tgcacaatcc cgaaagcaac atgaaactgg cttcaggcat cgctccggtg ccacggctgc
      301 tggaccgggg agtgaacgtc gccttgggca cggacggctg cgccagcaac aacgatctgg
      361 atcttttcgc cgaaatggac acgtgcgcca agctccacaa ggtgtcccgc atggacccga
      421 ctgtgctccc cgcgaggagc gtccttggga tggccgtcgg gagcggcgcc cgagccctgg
      481 gtttcggggc ggacctgggc gagatctcga cgggaaagtt ggcggacatc atcgttctgg
      541 atttcaaccg cccccacttg actccccttt acgacaccac aagccacctc gtatacgcgg
      601 cccggggagc cgatgtccgt cacagcatca tacatggtcg cctggtgatg gaagaccgac
      661 aagttctcac cctcgacgtg gatgaggtca tggatcatgt ccgaaatctt gcccgagccg
      721 tgcgcaccac cccgggatga tgccgtctgc cgggaggtgg acctgctcct tttgaatgcc
      781 gactgggtcg tgacgtgcac tcaggaggcg ggctgcattc cccggggggc ggcagccgtc
      841 cggggcgaca ccctcgaggc gctggggccg accgccgtgc tgcggcgaca attccgaggc
      901 cgccgggaga taaacctcga ggggcatgtg ctcatccccg ggttgatcaa cacccactgt
      961 catggagcca tgagttgcct gcgggggttg gaggacgacc ttcccctgga ccgctggctc
     1021 catgaggtca tcttccccgt cgaggccgca gtaatgaccg aggaaaccgt ctactgggga
     1081 acgctcctct cggccgtcga gatgatccgg aacgggatca ctaccgtttg cgacgggtat
     1141 tttttcgagg aggccgcgac ccgggcgatt ctggattccg gaatgcgagg cgtgttggga
     1201 caggggatcc tggattttcc cactcccgac tgcccggatc ccgcccagtc ccgcagacgg
     1261 gccgagtcct tcctggattc cttccccacc gagcaccccc gggtacgccc ttccctgttc
     1321 tgccacgcac cctacacgtg ttcttcccaa acctttggct gggccaaggc cctttgcaag
     1381 gaacggggaa ttctttttca gacccacctc tccgagacgg cctcggaagt gcgggacctc
     1441 acggaacttc acggcgagcg tcccgttttc tacctggatc gcctgggaat cctggacgaa
     1501 caaaccctgt gcgtgcatgg ggtctggttg agttcgccgg aaatcgacct cctggcccgc
     1561 agaggagccg ggatttccca taatcccgaa agcaacatga agctcgcctc gggagtgtgc
     1621 cctctcacgg aattcctgtc tcggggagcc cgggtaggcc tgggaaccga cgggtgcgcc
     1681 gggaacaacg acctcgacct tttctccgag atggaccggg ccgcaaaact cgagaaggtc
     1741 ttccgcgcgg acccgacctc cggcgccgcc gtccggatct tcgagctcgc aaccgcgagc
     1801 ggggcccggg tgctcggatg ggaggacgaa ctcggaagcc tcaggcccgg aaagaaagcg
     1861 gacctggtcg ccgtcgattt ccgacagccc cacctgacgc ccgtctacga ccccatctcc
     1921 cacctggtct atgcagtcag aggaagcgac gttcgccatg tgtgggtgaa cggacggcag
     1981 gtgctggcag atcgacattt ggtgggggtc gacgaggccc gggtcatgga agaggttcgc
     2041 aaggttgcgg agcaggtcag gcacattcgg tccgggggac cccgagcctg aatcggtgcg
     2101 ccccgggttc ggacacatcc gtggtgcgga cccaagacga gtcccgtgga ttttctcaaa
     2161 caccgttcta taattatttg ccatgtccgt aaggattcca ttgcaggtca tcccacaaca
     2221 aggaacgacc tttgatgcgc gagaaacaca agatcttgat cgtggacgat gaggccccgt
     2281 ttcgttcggc actatgccgc ctgctcgaaa agcaatacat cgtggtggag gcgaagaccg
     2341 gcgagaaagc tcttgagctg gcctaccggg aagaaccgga cctggtgctc ctggatatcg
     2401 gccttcccga cgtcagcggc ctggacatcc tgcctcgatt caaagaactg cggccgtctc
     2461 ccaccgtggt catgatcacc gcttacgaac acgtgaagga cattgtggcg gccatccgcc
     2521 tgggggcttt tgattacctg gtcaagcccg tggacctgga cgaattcgag atgaccgtcc
     2581 agaaggccct cgagcatgcg gggctcaaga acgaggtgga tcggctgcgc caggaggtcc
     2641 agcggctcca gaaagtggat cggttgatcg gcagaaaccc caaattcctc gacgcgcagg
     2701 tactggccat caagagcgcc cagagcctgg atgcaggggt cctgatccag ggacagagcg
     2761 gagtcggaaa agagttgttt gcacggttga tccacgccaa gagtccccgg gcggcttatc
     2821 cgttcgtggc gttgaattgc gccgccttca gctcggagat catcgaatcg gaattgttcg
     2881 gatatgagaa aggagctttc acgggagcca gggccgaggg aaagcaaggg ctcctggagg
     2941 tcgccaacgg gggaaccctg ctgctggacg aggtggtgga gctgcatccg gagattcagg
     3001 ccaagctctt gcgggtgttg gaggagaagg aattctatcc cctcggcggg actcgaagga
     3061 gaaccgtgga catccgaatc gtgtcggcct gcaaccagga cttgtggcaa gcggccgaag
     3121 gcggccgatt tcgcaaggat ctctactttc gcctggccac cattcgcctg gacctgccct
     3181 ctctgaggga acgccccgag gatatccttc ccttggctca attcttcatg gaagaattca
     3241 acgacaagta cggcaaacat ttccgccgac tgagccccga ggcggaactg atcctgctca
     3301 ctcacccctg gcaggggaat gttcgggagc tccgtaacac catcgaacgc gtcgtgctcc
     3361 tggaaaacga cgaaaccatc ctgggccgct acctcaattt tctgggtccc cagtcgggcg
     3421 agccgccggc gggaacaggg ggcctgcgca tcgagctccc ggaggacggc attgccctgg
     3481 aacaagtgga aaagcacatc atcttacagg cttacgagaa atgtcaccga aacaaatcca
     3541 aaacggcccg gtaccttgcg ctccccaggc acattttgat ctaccggctg aagaagtacg
     3601 ggatcgagga ttgaagaata gtctccaatt cccgacttcc ctggtcaata gtatccactg
     3661 agacgtccat ctaatggaat ttattcgtgt ttctctgcct gggatctgga gggccgggat
     3721 gggccgcaag cataatgtgg atactattct tcacctcttc ccccccccat ccgggaccgc
     3781 ggggaacgaa ttgcttccaa tccctcatga aacctcgcta ttggcttctt ggcacgcact
     3841 ttgctcttgt tgacaatcgg aggctgaccg tggattttca aattcgggtg ttgttggttg
     3901 aagatgatcc tcccttgggg cgctctttga taaacttttt ggaacgggcg gggtatgtct
     3961 tcgagagttg ttcgactgcc tgcgaagcga tcgcgctggc ccagcagcat cgacacgaca
     4021 tcgtgatctc ggaatttcat cttcccgatg caaacggagt gcgactgttc gagcagttga
     4081 agcgggttgt tcccaatgcg aaagcgattc tgatttccga gttcgatttt caagcggttg
     4141 cgagtgaagt ggggcaagtg gaggtccatg catttctgaa aaagcctttc gatttggccg
     4201 aactcgaaac agcgctatct ttctcccatg catacagttg aagcgaaagc tcaagtgaag
     4261 cataccggtt gaagtgattt ttcaggttct tacggttctg tgaggacata cctgcctccg
     4321 tttacgtgta gggaatctca gaagcgaacg tgacggccga agtggaattc gcgtgggtta
     4381 tcgagaatgc aggctcaggt gaaggcgacg ctggaacgct actgttcaag ccgcagcgca
     4441 tgttgacgtg atggttcctg tgatctttgc ggaatttccc ctctcccatg gtatcggaca
     4501 cagcggtgcg agtgaggttg gatctggatc tgaggtggtc gcaagtggct gaaactcctg
     4561 ttcccttcta atgctgacaa ttctgagatc tccctgaacg atagcagagg gccatgagga
     4621 acaaccttat ggccttctgc atttcgtttg gctagaatga aagtacgtcc gtgaggttcc
     4681 cggatccttt gtttgagatt tcggggattg ttgaaacggg gatgaacgaa ctcacaacga
     4741 aggagtaaga ccatggtggc acagaagctt gaattgattc agaaaatgga acagaaaggg
     4801 atgaccgtgg cacaagccgc agaggcaatg ggctttgacg ctcaattact gggattgtac
     4861 cttgtcaagg acgcctaccc tgtccctaaa aggatattgg acaaactggc agaggtcatt
     4921 tccaactagg agggacggca atccttcacc cggcgatccg aacctttgcc acggtcgttc
     4981 ggaaccattg tcccacgctt gaactgagct attgagaccg tttcggtcct ttccccctcg
     5041 tgatccccgc cgcgaggcgg agaaggacac agatcgtttc cacagcccga cgacccctac
     5101 gccgcctttc ccccaccaac cctccccctt tcttgcgaac cgccgcaacc cttgcgctcc
     5161 cgggcatctt acgggccgcc caggcgcccc aaaggaccga cggcccatgg gtgtgcgatc
     5221 ccggatcccg ttgctctctt gcggggagac acgggtgctt cacctccggg atccttgccc
     5281 atgcccgcct tgccggatat gcacaaactg cacagacttt gccccctgca caaaagtact
     5341 tatttcacgt agccccgatc cccacccttg acaagaacat cggatggtgt tagcagtagc
     5401 cgcgtatcgc ggacgaaagc aactcccctc tcttacatgc acctcccctc tcccaggtcc
     5461 tggcaccgct tttgggctta tcgttgtgtt ggtttcaccc tggcacgccg acgcgcattg
     5521 cagcaggcag gatcgtgcaa gggagtagga ttgcacatgg gcgataacct gggttatccc
     5581 tgcgtggaca ttggtgatac cttgatcagg aggcacacat gaaacaggaa aaagtatttg
     5641 cacaattgga gcaaaagggg gtttcacgaa gagatttcct caagttctgc agcttcgttt
     5701 ccgccaccat ggggctggcc ccttccatga cccccaaggt tgccgaagcc ctcacagccc
     5761 cccaacgccc acccgtggtc tggctttcct tttcggaatg caccggctgc cccgagtctt
     5821 tgctgcggac cacctatccc tggattgacg agttgctcct ggaaatcatc tccttggact
     5881 acagcgagac ggtgatggcc gcttccggac aggcggccga ggacgccctc catgccaccg
     5941 tcaagaagca ccccgggaaa ttcatctgcg tggtggacgg cgccatccct acggcggaga
     6001 acggcgttta cgggatgatc aacggcaaga ccatgatcga gatcgccaag gaaatctgtc
     6061 ccaaggccct ggccaacatc tgcatcggaa cctgttcttc cttcgggggc gtcgccgccg
     6121 ccaaacccaa tcccacgggg gccaaggacc tggaatcggc cctgggcccg gcccttacca
     6181 cgcccgtggt gaagatcccc ggctgtcctc ccaacggcat caacctggtg gccaccatcg
     6241 tcaacttctt gttattcgga aagctccccg acctggacgc caagaagcga cccctgttcg
     6301 cctacggaaa aaccatccac gacacctgcc cccgacgctc ccactacgaa atgggcgagt
     6361 ttgtcacgga attcggttcc aaggaggccg ctttgggcta ctgtctattc cagttggggt
     6421 gcaagggccc ggaaacgtac aacaactgcg ccatcgtcaa gtggaatgac gggaccagtt
     6481 ggcccgtgca ggcggggcac ccttgcatcg gatgcagcga acccgatttc tgggacaaga
     6541 tgtctccttt ttacaagtcc ctgtagaacg gggccggagg tccgactcgg caattcccac
     6601 aaggacggca ggaagccttc accatcaata aaggagaagt gagttatggc acagcgaatt
     6661 gtcgtcgacc ccatcactcg tattgagggt cacttgagaa tagaggtgga agtgggggac
     6721 ggaaaggtaa agaatgcatg gagcagttcg accctcttcc gcggccttga gatcatcctc
     6781 caggggagag acccccgcga cgcgtggctc tttacccaaa gggcctgcgg ggtctgtacc
     6841 tacgtccatg ccctggcgtc ggtgcgatgc gtggacaacg ccgtcggcgt cacggtcccc
     6901 gacaatgccc gccttattcg aaacctcctc atgggggccc agttcctcca cgatcacatc
     6961 gtgcatttct atcacctcca cgccctggat tgggtggacg tggtcagctc cctcaaggcg
     7021 gacccgaaga aggccgcggc cctggcagac caggtgtccc ccgccggagg caacgtgaac
     7081 gagttcaaag cggtgaaggc ccgggtgaag aagttcgtgg acagcggcca actgggcctg
     7141 ttcaacaacg gctattgggg acatccggcc tacaaactgc cccccgaagt gaacctcatc
     7201 gcggtcaaac actacctgga cgccttgcgc cagcaggcgc gcaccgcccg gatgcacgcc
     7261 gtattcggag ccaagaaccc ccaccttcag agcctggtgg tgggcggagt cacctgtgtg
     7321 gcggacctca atgccgatcg cattgcggag ttcaagtatc tctggaagga gaccatggac
     7381 ttcgtaaaca acgtatacat cccggacgtg gtcgccgtgg ccgggttcta caaggactgg
     7441 ggaaagatcg gcggcaccac caacttcctg gcttacggag aattccccca gagcgccaag
     7501 gaacccgaga gcctgctctt cccccggggt gccgtcttca agcgcaagat ttccaatgtc
     7561 caacccgtgg acatcaacct catcgaagag catgtcaagc gcagctggta cgaaggggac
     7621 aaggctctga atcccgccgc gggagagacc aagcccaagt acgagagcct ggacgtcaac
     7681 aatcgttaca gctggatgaa agcgcccaga tacaacggcg aacccatgga ggtcggtccc
     7741 ctggcccggg tgctcattgc ctacggtaaa ggtcacgctc ccaccaagaa ggccgtggac
     7801 ggactgctca aggccctgaa ggttccccag gaagccctgt tctccaccct gggaagaacg
     7861 gccgcccgtg ccctcgagac caagatcatc ggcgaagcca tggaaggatg gatgggccag
     7921 ctggtggaaa acctcaagaa aggggacacc aagacctacc aaccctacga aatgccggcc
     7981 caggcctcgg gagtgggcct caacgacgta ccccgcggcg ccctgggcca ctggatcgag
     8041 atcaaggacc agaagatcgg caattaccaa ctggtggtgc cttccacctg gaacctggga
     8101 ccccgctgcg ccggcgacaa accggggccc gtcgaggagg cccttatggg cactccggtg
     8161 gcggacccca aacgcccggt ggaaatcctg cgcaccgttc actcttttga tccctgcatt
     8221 gcctgcgggg tgcacgtgat cgacccgcag accaacgagg tctacaagtt taaagttgtc
     8281 taggcgttgt cgccgagata tgctatcgtc aaacaggaac ctggctcagg agccgggttc
     8341 ctgttttttg ccccgatcgg tttgccgaga cgcgaggtcg ttctatgagt gaaaagcaaa
     8401 tcctcgtcct gggtgtgggc aacctcctgc tgcaggacga aggcgtggga gtccgggtca
     8461 tcgaaaccct cgaggcggga tacgctttct cctccaacgt cacccttctg gatggcggga
     8521 ccctgggttt gagactgttg gatcccatct cccgggccga ccacctcatt gtggtggacg
     8581 ccgtacaaaa cggagaagcc ccgggcactc tctacttcct ggacggcaag aacctcttca
     8641 accgggtcac cttcaagaac tcgatgcacc aactggacct ccgggaagcc ctcgcgtatg
     8701 cagaggtcct gggcaattcc cccaccgccg tggtcatcgg gatcgagccc ggagacattt
     8761 caccctgggg gctggaactg accgaatcgg ttcaatcccg tctcgaggac atggcgggcc
     8821 ttgtactgaa agagattgaa aaagcaggcg gcacctatgt tgccaagaat tcccccggag
     8881 ccgctctcca atgagaaagg aaccctccca tgtgtctggc aatccccgct gagattgtgc
     8941 aaatcgacga tgaaacggca acggttcgag tgggagaggc ccttcggaag gcatcgctca
     9001 tgttgctgcc cgagggagcc gacctgggcg attacgtcat cgtccacgcg ggttttgcac
     9061 tgcacaaggt ggacccggaa gaagccatgg aatccctaaa gctcctccgg gaactggctt
     9121 ccttcggaga agaggagtcc ccccttggga cctgacccgg gcggaattgc ctttttgatt
     9181 ttcccctaga tcgagcgatt tgaccgggac taaccccgtt tcgtcgttcc cgcccaggcg
     9241 ggaatccgga aagagcgtgc atacccaatg gatgcccgcc ttcgcgggca tgacgcccgg
     9301 atgctctcgg aatcgctcaa ctcaggtttc ctcctttgcc ggttcgaatc ccggtaacct
     9361 ccgggatatc ttcttctcct gccggaatct tcaggaccga tcggagggcg ggcgcctgcg
     9421 ttgcgccgga accgtagggg ccctcgtctt ccctgctccc actttctccg gggaagaaac
     9481 gggtttccgg aagccgacga ggactcgcgg atcgtcaacc gcctttgccg ctctcatccc
     9541 ttcgtgccgt cgccgaccga cgtcgagccc ggcgggggcg ttccaaccgt tcctataatc
     9601 cagcagttga agccattcca acttttgatc cggactgccg aataaggatg cattcccgtt
     9661 gacatcccat tgaagcctga aataacggca tccgtcttcc cgcagcagat ccgcatcgca
     9721 ctccagacct cggaggcccg ccaggagacg cccccggaga tttgtcccgc tactcgtgtt
     9781 cgccccggtt cttctcctcc tgaccagtca ttcttacgag tcggcaggga atcgttcttc
     9841 ccatctccat tcgtaagcgg acggcatgat cgcttcccta cagtccccaa ccatcgagga
     9901 agccttgggg ttggaacccg ggaccgaggg gatttccgat gcggagacca ttgccgggga
     9961 aggctccacc caggacgatg gggagccggg ggagttggcg gaagcgccgg atgacaggga
    10021 ggatcctgcc cggcagggcg ttgcccccga tgggcgtgag ccccttctcc ttgtgagggc
    10081 cgcagacgcc aaggctcctg ccgtcgccga cccatggccc gaactgccgc ttggagacga
    10141 cgacaaagtg gtcaaaaacc cttacgtcgt caccggcaaa aaagatcccc gtcattacgg
    10201 catcttctgg agcacgctgc tgtacaactc tttcggcaag tggctggaga gcccttacag
    10261 caaccccaga tccggtgtgg ccaagaggtg ccgggaatgc cgcatggttt cccccaccgt
    10321 ttggaaaagg ggaaaagccg gtcaacgcgg ctcccgacag aggggggaat cgaccggaaa
    10381 cccggaagca tccactcgca ccggaccacc gtcgacccaa acctcttgca aaattccctc
    10441 acgatgaatg catgggccaa acggttggag ggaaagatcg tggtggatgt gaccctttac
    10501 aatgacaaga cggggcatca tgttccttcc gattgcttgc cccttgaggc accctatcct
    10561 ggtggtgaag gcgacggatt ccgctggaaa gccactccag caggtgagcg gttccgtttt
    10621 gcctccctgg ttcggaacgg agggagaaag agacgggaaa ggagacgatg gcgtggagta
    10681 cgcacaatcc ccgaaaagcc ggaggatcga tttgcccggc tctgcctgga accccgacag
    10741 gaagtatctt ggaggactcc ccggcaagac ctacgccaag ctcctgcagg agttgcggac
    10801 caatgtcttt cctacggcct cctactggag gcacaccacc ctcgtgagcg acaaccggct
    10861 tgccgccttc gagcgggaca cctccggtta ttccttcttt cccgaaggag ccggagagat
    10921 caccgggacg aacacccacc tttaccgccg cgattcatcc aaccgatgga ccaaaagaag
    10981 agggcgatgc cggacatcgt catggcccgc aacgtgccgg ttctacggca ggagtgttga
    11041 atccttccta tggacgccct tccttggcga ccacctcgtt tgcgaccggt ttgaacagga
    11101 aagaaaccct gccttcgtcc gaaggatcat cgaaggtcaa cagcaccccg actttgtcgc
    11161 cgtcccagaa gtagcgtttt tccgattgga cgggatggta ggcatttccc agctgttgat
    11221 agaagcgctc cttgatcttc gagaaggcat ctgcggtcct gtaatatatg atgacgttgt
    11281 aaaagtgtcc cttgtgaaag ccgtaaacga tgttgttcac ttcgacgtcc tcgaagctga
    11341 gctggtcgcc gtcccgcaca tagaacgtca ggtctccgtc ctcggccagg agtttcaccc
    11401 cgggcacttc gccaaccgag gctccccaag ggattccccg aaaccggtcg aattccttgg
    11461 gaaccggggg gctgttcagg acgacggcca gcccgcccag ccctaccagc aaaacacaag
    11521 ccaggatcat gaggatgagc cctttgcgcc cacctgaagc catgcttttc tccttgtctt
    11581 aaaaaagacc cggacgatgc gccctcgccc gtgcagcccc ctcgggggaa cgcccacctt
    11641 tatgccgccg ccgggaggtc tcgccgcagc cctagaaatc ggcacttccc ggagaccggg
    11701 gaaaaggaat cgcctcccgg atatttgcga cgccggtcac gaactggacc atgcgctcga
    11761 ggccgagtcc gaacccggca tggggagcgg acccatactc ccgaaggtcg agataccagc
    11821 ggtagtcttc gacggatatg cccttctgtt tcatctgatc gagcaagaca tccggccgtt
    11881 cctccctctg ggagcccccg atgatctccc ccacccgcgg gaccaggatg tccatggccg
    11941 caacggtctt gccgtcgtcg tttctccgca tgtaaaaagg cttcaaatcc cggggaaagt
    12001 ggacaacggc caggggtccg cccacgtatt tttccgccag gtaccgttcg tgttccgcct
    12061 gcaggtcgct tccccaggaa accggaaagg caaagggttc cccggacttg cgaaggacct
    12121 ccacggcctc gccgtaggtc atgacctgga agggctcccg ggtgagctta tcgagggatt
    12181 ccatcacacc cggttccaca aaccgggcaa agaaacgcag gtcctcctcg caatccgcca
    12241 gcaccgcttg cgctaggctc ttgagaaagg cttccgccaa ttcgagatcg tcctcgagat
    12301 cgtagaaagc catctcgggc tcaaccatcc agaactcggc caagtgtcgg ctggtgttgg
    12361 agttttcggc cctgaacgtg ggaccgaagg tatagacttt gcccagggac agggcgtaga
    12421 tttccgcctg gagttgaccg ctcaccgtaa gatacacagg ttttccaaaa aaatcctcct
    12481 cgaagggatt ccctccctct cggagcgccg cagccgcttt ctgcaggtcc agggtggtga
    12541 ccctgaagac ctcccccgca ccctcacagt cgctggaggt gatcaccggc gtatggatcc
    12601 aatagaatcc ccgatcctgg aagaaccggt gaatcgcctg ggagagtctg tttctgaccc
    12661 gggtgacggc cccgatggta ttggtccggg gcctgagatg tgcgatctga cgcaggaact
    12721 cgaaggaatg ccgcttcttc tgaaggggat atttggccgg gtccgccccc ccatagacct
    12781 ggatccgatg cgcccggagc tcgatgctct gttcccgccc gggggaagcc aggaccgtac
    12841 cttccacccg aaggctggaa ccggtggtga cggaacccat ggtaggtttg tgctctccca
    12901 aggcggtttc cgcaaccacc tggagattcc taaaacagga tccgtcgttg agctcgataa
    12961 acgtcactcc ggccttggaa tcccgccggg tcctcaccca gccttgaacc aggaccggct
    13021 ttcccaggta ttcgttttcc cggcgaaaca ggtcggcgat gcaaattccc ttctccatgg
    13081 cgcagggacc ctttcgtgcg atttccgggt tcggttcgat cacggcccat ccgggccgga
    13141 gcacgccccc cggttccggt gcggcgatcc agcccattgc atccgtttct cctcggcgaa
    13201 aacggcaagg gcggccgagc gcatcctcct ggggggccgt ttgcgattgt agcacagcct
    13261 tcccaaagaa caaaccgtgc gcggccgggg ctttcggccg gatctccttg tccatggcgt
    13321 tttcccggca actgtgctag tagagcccct ggaaggttgg tgtttcaccc caggaaaacc
    13381 tgaggacccg tgtcgagaca attctatcag accctcttgg aacaaatcgt cacctggtgt
    13441 ctcaatgccg gaggctggtt gttgctgcct gcggtctgtt acggtctgag aaaccatggg
    13501 gagttcctca aaggcaggct ggggaatttt tccttggacg ccgcagaccg gaaaggaccg
    13561 cgaatttggt tccatgcggc ttcggtggga gaagtcaacg gagccgtccc catcctgcat
    13621 ctcacccaca ggcacctgcc ccgggccttc atctgcctca gcgtggggac tccccaaggc
    13681 catgaatttg ccaaatccca actgcctgcc tgggcgacgg tcgttccctt ccccgtcgat
    13741 gtgccctgga ttgtggacaa gagcctgaaa aggattcggc ccgatctgta cgtgggattc
    13801 gagagcgagt tctggcccgt cctgttgggc cgcctgaggc gcgcaggagt cccggctctt
    13861 ctcctcaacg gccgtctctc ccggcggtcc gccgagaagt atcgcttcct tcgcccggtt
    13921 tttcagcctc tctttcagca ttttcgccgc atagccctgc attcggaaca ggatcggcaa
    13981 aacctgctcg atatgggggt ttctccggac cgaatccggg tggtgggaag ctccaagcac
    14041 gacggcctgc ggctccgggt gcggcccgag gccgcagcac gctggcgggg actcttgggg
    14101 attcccgaga acacgcccgt cctcttgggg gggagtcttc gccgttcgga atgcaccggt
    14161 cttctcgagg tcttctgcga gcttcgtaaa accacggatt cgtgcctggg gatctttgca
    14221 ccccgacatc tccatcaggt gctccccatg gccgagtggc tcacgaaccg cggggtgcct
    14281 tttcagagac tgtccgaaat cgaggggcgg cgcacacgaa gagaggcccc cgtggtactg
    14341 gtggatcgca tcggggttct cttcgagctg tacgctttag gggacctgat cttctgcgga
    14401 ggcaccctcg agcccatcgg gggccacaac atcctcgaac ctgcggcctg ggccaggccc
    14461 gttttctacg gtcctcacgt ggagaaggtc cgggaagagc accgggtcct ggcgaaaaag
    14521 aaagggggat ttcccgttcg ggatttcgac gacctccgtc agcagtgggc atactggctt
    14581 cgacatcttc ccgagttgaa gagctttggg gaaaatgcta aagatgccct ggactttctt
    14641 gggggggctg cggaaaaaca ggtggagctg ttgctgcaga ccctttccga atccggtgcg
    14701 agggtgggtg catgacgacg gatcgcctcc aaacccttct ccaggaattc aaagacggga
    14761 aaaagaccct ggaggaggta ttggattttc tcaagagact tcccttcgag gatctgtctt
    14821 tcgctcgagt ggaccatcat cggggccttc gtcgcgggtt ccccgaggtc gtctacgggg
    14881 aagggaaaac cgcccggcag attatcgata ttgttggggc catgcagcga ttcgggagcg
    14941 acatcctggt gacgagggtg gaggagggca aagctcggga agtcgcctcc accgtccccg
    15001 gaatgacata ccatcccctg gccgggatcc tttccttctg ccaggaaccc cacggcgaga
    15061 aggccccggg gagcatccgt gtcctgtgtg cggggtcctc ggacatcccc gttgcggagg
    15121 aagcggccgt gaccgccgaa ttcatgggca atcgggtggg tcgccactac gacgtggggg
    15181 tggccggact gcaccgtctg ctgcagattt gggacgagat tcgggacgcc tcggtttacg
    15241 tggtggctgc ggggatggag ggagccctgc ccagcgtggt ggccggcctg gtggaccgcc
    15301 cggtgatcgc cgtcccaacc agtgtgggat acggagcggg cttcggggga atcgcagctc
    15361 tcctgggcat gttgaattct tgcgccccgg gcattgctgt ggttaacatt gacaacgggt
    15421 ttggcgccgg ttacctggcc ggtctgatca accggcgctc cggggcagga caagcccggg
    15481 cagagagcga aaccgcctga atcgccggag gaggcgtcgc agccttttcc gcggataccc
    15541 cccggtcccc tccagggttg gaagtggagt ccggagagaa aggaacgagg aaaatggcga
    15601 gagaataccg ccccaatgtc cgggagtccg ccgtgatatc gaggttggac cacgccaagg
    15661 aatcccagcg catcaatgcc ttgaacctgc ttcgccagca tctggaggaa gtaagccggc
    15721 gcatcgttac gaaactggtg gaagaaaggt tggtggagac cacatcgatg gatgacctgg
    15781 agggccagat ccagcaggcc ctgcagacca tgctcacttc agaagaattc gagatccagt
    15841 accagacggc caatttgagg accctggtcc cccgtcccca tttcatcagc ctctatgtga
    15901 cggccttcat cgtggaaaag ctcatcgatc acccgagcgt caaggagata ttcggcaccg
    15961 acgaggacat ctatgcatgt gtgaatcggc aggtttcccg gttcataccc ctccagtagc
    16021 cgctttccac cgaattgctc catccggttt ccggcggacg tgcagactcc cgggctcagt
    16081 ccgtcacgga cccatccccg ggccgtgagt cttttttttg tcgccgcccg gagcgttttc
    16141 cgcgcccgac acggcccttc gtgcctgaag ggccaggtct ttgagctcca agagatcccg
    16201 gtacgaaaaa tcagcccgcc tgcaggcaaa aaaagaattg ttgtgataca agggctcgtt
    16261 ttcgatatca aaggacgaaa cctgcaccgc atccttccac atggggcttc ccggcacggg
    16321 ggaatattcg gccaagaagg gactcaaccc ttccctgcga accgcattga cggcatcggc
    16381 cacctcccgg ggggactggc cgggggctcc gcacaagagg tagacaccga tacgatcctt
    16441 ggaaaacccg gcccgggcca ggttccggac cgcttcttcg aacatgtccg cctcgacctt
    16501 tcctccccac tggcgatgcc tctggggctg ggtggtctcc aatcccaggc ggagagtggt
    16561 aaaaccgccg gcgaacagca gatcacacca ctcgggcgtg agggctcgga cgtgcacggc
    16621 gttgggggta tgaaacctca gggcaacccc gtccctgcag agcctttcca gcgcgggcct
    16681 caggctcgat tccgcatgga gcaaaagggc gtcgtcgtag aaggcaaaat ccacgacccc
    16741 tcgctcgccg tgccacctgg agatctcacg gtggatgtcc tctccgctcc tccgttccca
    16801 tcggggctcg agtcgagccg acgcacagta agggcagcga aaggggcatc ccctgcccgc
    16861 gagaatcggg acatattcca gaagcggaac aaggtcccag gcgggagacg gcaggaagct
    16921 caagtccccc catcggtcct tgttgcggac cggcacctga agagtgtcct ccaccctgcg
    16981 cccgatgcgg gggagggggt cggtcaccac caggtcggcc ccgctggtgc gccgggcatg
    17041 gtcttcgcac agccgggcgt aaacgccgcc cagccagatg gggatttcgg caaagacatc
    17101 gcgcaaagcc ccgatggtct gctggacacc gggataccaa taggtcatca tggaggtcac
    17161 ccagatgagg tccggtttcg gaagtgcccg gagcttttgt cgaaagcttt cgggatggat
    17221 gccgtggcgg tagtaaaacc gggggaaatc ccggtaaggg gagggctttt cgatcttcat
    17281 tttggggtac ttcccggtcc cgtaactccg ggacaccccc ggcttcatgt ccggatgggt
    17341 attgctgaag ggatcgtacc ggtcaaggca atccaccaag gcgacctgga tgccgttttc
    17401 ccgcaggagg gacgccaggg tcagcaaacc caggggcttg gaccacagat cgtaggcggc
    17461 aaaatccgtg atccaggggt tgactaagag gacaaaggga gttcttggca tacgagatcc
    17521 gccatgcgga acccgccggg ggtcgatccc aaggcgggcc ccaacggaat ttccgtaaag
    17581 agtccggtga aagaagttca catccttata gctccgggac cctgcagggt caacttcggc
    17641 aggaattgtt tcccgaaaac ctattcctgc gggacctttg caccctttcc taatcgtgtc
    17701 ccctccggca agaccgagac caaggagact atatgagagc cgtggttcaa agggtttctc
    17761 gagcccgggt cgagattcgc ggggagaccg tcggccgggt tgaccaaggc ctggtggttt
    17821 tcctgggagt cggccccgac gatggtccgg aagacgcccg gtccatggcc gacaaggtcc
    17881 tccacctgag gatcttcccc gacggcgaag acaagatgaa ccggtccgtc ctggatctcc
    17941 gggggcagtt gctggttgtc tcccaattca ccctgtacgg tgactgtcgc aaggggagaa
    18001 ggccttccta tgcgggagcc gcccctccgg agaaggcccg gctgctctac gaagccttcg
    18061 tttcggaact tcgtgggagc ggcctgggcg tggaaacggg acaattccag gagcagatgg
    18121 aggtccacct ggtaaacgac ggacccgtga ccttgctgct ggacagtaag aagaaattct
    18181 gatttcagga agggaggttc ccgactatcg accgggaaac ccgcgcagac cctggatccg
    18241 agaagtggtc ggcattcagg aaggaatcgg gcgggaactg aggaaaggtc ctgccggtcg
    18301 aggggatgag gcgggccatt acctcccggc gaattccgtc aaggggtaat gatgggttgc
    18361 ccggcgcagc aggatcctcg tcaccaccgc catgagttca tgaaaatcgg catccgcgta
    18421 ggttaggaac cggggacgaa ggccatgccc ccgggcaacg gtgtccgcgt cggaatccgg
    18481 caagatcagg acgatgggaa ggtctcggaa atgctccctc atcttcagca ggtcggaaag
    18541 atccaactga gttcggggga aaaagacccc ggccaacaga tcataggagc cgtggatcat
    18601 ggcccggcgg agctgctcaa tactggagca tgtcagaagc ctctcccgat gaatgatgtt
    18661 tcgaaggaga ccatccagaa tccgatgggt ctcatcctgt gcgggagaaa agaacaacag
    18721 gtccatggag gccgtttctc gatgaagggg ttatgggtgt cgtccaatca atagaacccc
    18781 caatcaagaa ttgtgcccaa agggttttca tccagtttcg ttagtggtta caggcgattg
    18841 gaagggggcg tctccgccgg agaacccgaa attgggcaac cgatcgtcca acaggccgga
    18901 cgcattccca taaatcggac agggaccggc cggaaattgt ctccccgaaa gggaggcgga
    18961 gtcttgtctt atgggacttt tggcaccgag gacggctgta gaggttcgac gacaagcgga
    19021 gcggggccgc gtttcccgct gcagggggtg ccgaggatgc gcgtcttgca gggtccgtgg
    19081 gggctaacga gacgggaagg cccgggaggg ggcgaggatt tcaagccgga tcggcgatgt
    19141 tgtattcttt cagaagctca tagagcctgg atcgggacaa tcccgagatt ctacaggctt
    19201 ccttaatgtt gcgtcccgtg tgaccgacca agtgggtaag gtactgtcgg tccatggtct
    19261 cgcgaaactc tttgaaagga agaatggatt cggacctcag ggaggtctcg gcacgagggg
    19321 ggccgggtgc cgttttcttg ctcagcagca tggcggccag gtggatccgg atggaatcgg
    19381 gaaggtggat tggaaaaagg gtcggttcgt cacgcgccac ggccagggcc ctctccagtg
    19441 catggaacaa ttcccggacg tttcccggcc aacggtaggc cagaagggcc tccaggaaat
    19501 ccggggaaaa ccccttcagt cccagtctgt agcgttcaca caggttggcg aggtgatgca
    19561 tggccaggtc tttgatgtcg ctggggcgtt cgcacaacgg cggcagctct atggtaaagg
    19621 accggatgcg gaacagcaga tcgctacgga agctcccctc ttccaccatc ttttgcaggt
    19681 cccggttggt tcccgaaacc agcctgaaat cgctttcgac ttcgctcttg ctcccaatgg
    19741 gcctaaagcg tcgttcctgc agaaccctca gaaacgcctt ctgaagctgt aggggcagtt
    19801 ctcccacttc gtccagaaaa agggtcccgc cgtgagcctg cttgatcagc ccttcccgag
    19861 ttcgatccgc ccccgtaaat gcgcccttct catggccaaa cagcatgctt tcgacgatgg
    19921 tttcgggcag ggctgcgcag tccacaacca caaaaggccg gcttccacga gggctgtttc
    19981 ggtggatggc ggaagcgaac aattccttgc ccgttcccgt ttcccccgtg ataagaaccg
    20041 tggcgtccgt cccggccacc tgggcaacca gatccaggca ggttttgatc ctgggactct
    20101 cccccacaat accgcagcga tccaacgcca tcaggcgcga accggggctc ctgtcccgtt
    20161 tctcctgtcg atattggagg gcccgcacca agggaagcgt catctcctgg atggttgacg
    20221 gcttctgaat gtaatcccag gccccgctct tgatggcaag ctcggcccca ctcaatcggc
    20281 ctgcaccggt aatgatgatg acttccgggc gggacgccgt ttcggaaatc tcgggcaagc
    20341 cgtccagccc gcttccatca ggcaatccca cgtccagaaa gaccacatcg tattcgccgg
    20401 cgtgagcggc cgaaattccc tgggcgaggg tatgggcata ggccacctca tgccccatct
    20461 tcttgacgac ccccgaaagg agcctgcaaa tccccggatc gtcgtcaatg atcaagacat
    20521 ttgccatatc gagcctttca gcgttccgga cccgaagggt tccctaactc gtggcacccc
    20581 gacagcgatc gaccccatcc cgggaggctg acggtgcaga acgaagactc cggtaccgag
    20641 cgttcaccgc cccctcatcg tcccttgggg cgggaatacg gagactctcc ggtcatcgca
    20701 accctcggaa gttccggggg gcaaagccga gactcgcgac cgccgccccg ggggaaaggg
    20761 atttgccctc tagcctacct taaagaaggc ctttttgttg gcgccgcaga cgggacactt
    20821 atccggggcc tggtctccca ccgtgtatcc acatattttg caaacgtggt aatcctttac
    20881 cggaaatttt tcagggtcct ccagggcctt cgcgtacagg gctgcgtgga ccttttccac
    20941 ctcgttggca tagtgaaaac tgatttccgc cgccttttgt ccctcctcct tggcgtcgga
    21001 gatcatctgc ggatacatgt tcttgaactc gtgcatttcg ccggacaagg cctcttggag
    21061 gttctcccgg gtcttcccga ccccgcccgc aacccggaaa tgcttcaagg catggacggt
    21121 ctccgcagcc gcaacggccc tgaacaactt ggcaaccccg tggagagact cgtcttccgc
    21181 tctctcggca aaagccaggt atcgtcgatt ggcctgggac tcgcctgcaa acgcctcttt
    21241 caggtttttt tccgtcttcg tcgacatggc cttctcctac aggaatgtca atcgtgaacg
    21301 taccgcaacc taaggtcgca gcttaaacca aaccgtctca ttttgaaagg gattgtcgag
    21361 gcattgcccc ccctggccga cccgtcgcgg ccccgagtcg gcccgcactt ccacctcccg
    21421 gtgtcgctcc gtttcacaga gggctccaag cgccccggga ctcccttcaa aaaccgcggg
    21481 ggtttccagg tatgctttga ttccgggagg cggatatatt aagatgtgcg tacccaaaag
    21541 atcgcgatcc tgctcccacg gaccattcgc ccattcatgt tctcgacccg ggtacacgtg
    21601 acctttcccg gggggttgcc cgtcgttccg ggaaacggaa cggaattgca caaaccccac
    21661 gcaccggagg gaaagggcgg ccgcgttggt gcggatcatc tttcattgca ggaacttcaa
    21721 gcagactgaa accggttcgg taagagtagt gagcagtagg ggaaacagac cttctgattc
    21781 ccgccctccg aaaatcggcc tttcagggaa atgcatcatt gaattcgcca accatgaatg
    21841 tttgagagga ggaaccatga gcacaccgtt tcagccgggc atgacccatg aattgaaaat
    21901 caagtccaca ccggaacact ccgcccgagt tttctatccg caccttcccg atgttttcgc
    21961 cacccctttc ctgggggggc tcatggaacg ggtttccgcc gaactcatca atgaacgcct
    22021 tcaaccggga gaacagtccg tggggatctc catggacctg aagcacactg cagccacgcc
    22081 tctgggcatg gaaatccggg tcgtgaccaa ggtgacgagt gtggaaggaa gaaagctgac
    22141 tttccgactg gaagcctttg acgaagtcga gaagatcgga gaagcgaccc acgaacgatt
    22201 catcattcag gcggacaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
    22321 cctggtatcc caggccatcc acgactgcgg agaaaaaggg gtcaagggtg ccgtcgtcat
    22381 ctcgggcggc ttcagtgagg cgggaccgga aggggaagca cttcaagcgg aactggccga
    22441 cacggcccgc aagacgggag ttcgggtcct tgggcccaat tgtcaggggg tgaacaatcc
    22501 ctatggtccc ctgtgcgctt cctggcctct gctcacccgc aggggacgtg ttgccgtgat
    22561 cagtcaaagc ggcaccgtag gggctgccat gatggactgg ttctccgtag aggacctggg
    22621 agtttcgggg ttcgtcagct tggggaatcg agcggatgtg gatgaaatcg atctcatcga
    22681 atactacgag cacgatcccc atacgcgggt catcgcagcc tacctcgagg gcatcaagga
    22741 tccggcccgc tttcaaaagg tgatccagtc tttgaaaaag cccctcgtgg tcctgaaatc
    22801 cggccggacc ccccggggaa aagtcgccgc cgagtcccat accaagtccc tggcaggggc
    22861 ggactccgtc tattccgccc tatttcgccg gtacgggatc taccgggccg acaccatcga
    22921 ggaattttac gacatttcca aggccctcgc ctatctggaa ccgcccaaag gcaaccgggt
    22981 ccttttcatc accacttccg ggggggctgc catccttgcc accgatgcgg cggaacagga
    23041 aggaatcgaa atcccgccgc tccccgagga actgtcgacg cagctggaag gggtgatccc
    23101 gcctcatgcc atccgagcca atcccctgga tctgaccggc gacgccaatg ccaaaatgtt
    23161 ccgggaagtc atccagcgag cccgacctta ctacgatacc ctggggatcc tattcgggga
    23221 cccgattgtg gacgcctctc aggtggtatc ggccggggcc aatgaactgg tcgtcttcct
    23281 gggaggagcg gaggtggagc gggccgagaa gctcaaaatg caccagatgg gaatcccggt
    23341 ctatccgacg cccgagaggg gaatgaaagc cctggcctcc gtcatccctc ccgaaaagaa
    23401 aaccggaaga gcggccttca cggtacccgt cgcccggggg cggacccagt tgagcttttt
    23461 tgagagcatc aagttcctgg agagcaaggg tttcggctgc attgtgagtc gctttgccga
    23521 cagccccgga aaggcggtgc acgttgccca tcaactgggc ctgcccgtga gcctgcgaat
    23581 tgactccccc gacattctcc acaaatccga ttacgggggg gtgagactca acctcctctc
    23641 cgcccaggac gtgcgagccg cctataccaa gatgatggag gaggtgaaaa cccggttccc
    23701 ggatgcccgg atcaacgggg tggtggtgag tgccatggcc actcccggcc ttgaactgat
    23761 tctggggatg aaccgagatc cgcagtttgg acccattctc gttttcggcg tcggaggcac
    23821 tgccgtggag ttgtatcgag acgtctccat gcggctgttg cccctgaatc gcgaggaagt
    23881 gcttcagatg atgagtgaaa tcaagggagc tcctttgctc aggggctttc gaggcaggcg
    23941 tcccgtaaac gagaacgccg tcgccgacgc cattctgaaa ctggctcaag tggctgagga
    24001 aaacccggac atcgtggaga tcgacttgaa cccggtcttt gcctattccg aagatatcgt
    24061 ggtggtggac gcaagaatcc tgaaagcgtg agaatgaacc cgaggattcc ccgttgcgtg
    24121 accgaaatct tgccgacaac ttagccttgg gaggcaaccc atgcaggtcc ttgttctgta
    24181 ctattccagg ggaggaaata cccgcaagct ggcagaagcc gttgtccaag gggttcaaac
    24241 ggttccttcg gtcagggccg tcctgaggac taccgaagag gtgaccaaag aggatttgct
    24301 cgattccgca ggcatcattg cgggatcccc cgtttatttc ggcgtcatgg ccgccgatct
    24361 gaagcgggtg tttgacgaac acgtcggtat tcgaaagaag atggaaaaca aggttggagc
    24421 cgctttcacc acttccgggg atccgaccgg cggcaaggag acaaccatga tgtccattct
    24481 acagtgcctg ctcatttacg gaatgatcat cgtgggggat cccctgagcg ctacgggaca
    24541 ttatggagtt gcctgcacgg ggcctccgga cgaaagcgcc ctggaaaacg gacgaaagct
    24601 gggacaacga gtggcggagc tgtgtttgaa actgtcttcc tgaaaatggc ttcgcaaaat
    24661 cgggagttcg tcccgccgca tcccgtctgg gtccgcaaca gggaatgatt cgacccccgt
    24721 tgggcaaccc ggtttcgcaa cgaggggaag accacggtct tttccgagcg acttggggcc
    24781 gcggcatggg gacccgcttc tccatagagc ccgactcact gcttcttgag cgcctgcagc
    24841 ttgagcagat cttccgtgcg cagtgggtcg gggggtccgt tcttcagcag gtgggcataa
    24901 aactggatgt gagccgcgtg ctccagcttt tccagcctca tgcaggcctc tcgaaccgtc
    24961 ctcccaacgg taagggaccc gtggcgctcg agcagaagtg cttcatgtcc gccgatcaat
    25021 ccccggacgg catcgggaac ttcccgagtg gaaggggtgg cgtagggcgc ggtgggaacc
    25081 ggtccgatgg tgagccacac ctccggcagc aattcggcgc aaaacgggag acccgcaagg
    25141 gtgagggccg tgagcatggg gggatgagca tgcacgacgg cctcgacatc cggcctgttc
    25201 tcgtagacgc acaggtgcat acgcaactcg ctggaaggag tcccgggccc ttccaccggc
    25261 atcccggttt catccaggac aagaaggtcc gcgcactcga gatctccctt gtgccttccg
    25321 ccgggtgtga tcaggacata gttcgagtcc agacggcaac tgacgttgcc gtcggaagcc
    25381 gcaaccagtc ctttttcagc cagcctcctg caaacccaga ccacttcttc cctcaaatgc
    25441 atccttgaat tcatggacat tcctcgcacc ctctggacct cactccctgg acggaccata
    25501 ctttggtgct ttgcgtactg ctttttgcct tgtgtttgcc aaagcttcgt ttctatactt
    25561 ctaccacaca gcaacccata agcctatacg gcatgggaaa aaggaggaag cccaaatctt
    25621 atgatccgat ggtttagacg aaagggaaag acggttccgg ccgcccaagg gcccgggttg
    25681 caggaaccgg ggggctccac gctcgaaacc gaggagagtc cttccgagca ggcggcgcaa
    25741 ccgcagaaac ctcaagtcga ggtgcaaggc aaggaagtcg tggacgagga cctcgcctcc
    25801 tccccggacc ttgttcaaga agaagcagac ctcgatgaaa ccgaaggacc gagagatgag
    25861 aaggtctcct tctttagaag gctccgggac cgtcttcaca agacccgtac ccagctcgtc
    25921 aaccgggtcg accggctggt gttcgggaaa aaggtcattg acctcgaact cctggacgaa
    25981 ctggaggaag tgctcatcac gtcggacctg ggagtccaca ccacccagac gctgctgggg
    26041 gccatcaccg agagaatcca gcgtcgggag ctgagcgatc cccagaagct tcggggacaa
    26101 ctgagagaag aaattcaaag gattctgtcc gtggaggcac cgcccctgga cctgggcaag
    26161 acgcgccctt tcgtgatcaa tgtcgtgggg gtcaacggcg tgggcaaaac caccaccatt
    26221 ggaaagctgg ctcaccaatt caaggcccgg gggctgagcg tcatgctggt ggccgccgat
    26281 acctttcggg ctgccgccat cgaacagctt tccctgtggg gagatcgggt cggggtcccc
    26341 gtcatcaagc aaaagagcgg atcagaccct tcggccgtgg ccttcgatgc catggaagcc
    26401 gccgtatcgc gggaggtgga tgtggtcctg ttggataccg ccgggcgcat gcacaccaaa
    26461 gtgaatttga tggaagaatt gaagaaggtc caaagaatcg tggccaggaa gatgcccggg
    26521 gcaccccacg agacatggct ggtcttggat gccgtcacgg ggcaaaacgc tctctcccag
    26581 gcccgcctgt tcaagcagga aatcggcgcc acgggactca ttctgaccaa gctggacgga
    26641 acggccaaag gaggcatcat cgtggccatc tgcaacgagc ttcagctgcc cgtccgatac
    26701 atcggcatcg gcgaaggcat ggacgatctg agaagcttcg atcccgagga attcacccgc
    26761 gctcttttct gaagaagaag acatggacgg ccggttgggc ggcattcgag tcctcgattt
    26821 gtcccgtctc cttcccgggc ctttctgctc catggtgccg gcggacctgg gggccgacgt
    26881 catcaaggtg gaagacccca agctggggga ttacatccgc tggcggcccc ccctcatcgg
    26941 tcgaaacagc gccttccatg tggtcctcaa tcgcaagaag cgatccctca ccctcaagtc
    27001 cccggagggc aaggagctgt ttcgccgcca ggagatgaaa gccgacgtcc tgctgaaagg
    27061 gtttcgacca ggggggatgc ggcggctggg tctcgggtat gaatccctaa aaaccctcaa
    27121 ccccctcccg gtctattgtg caatcaccgg ttacggcagc gacggtcccc ttgcctcccg
    27181 gatccgtcac gacatcaacc atctcgcccg gagcggggtt ttctcctaca gcggacggga
    27241 cggaatcccc accctttccg gggtgcaaat tgccgacctg ggcggtgcag gcctcccggc
    27301 cgccttcagc attccggctg caatgttggc tcggctgaaa gtgggcgagg gccagttggt
    27361 cgacatctcc aggaccgacg gcgccctggc ctggaactgc ctccggtggg gaaagaccct
    27421 ggccgacggc gtcgtgccct gcccgggaga cgactttctc aatcacgggt tcgcgtgcta
    27481 caacctttat cgaaccccgg atgaccggac gatggccctg ggagcgttgg aaccgcagtt
    27541 ctgggaagcc ttttgcagca ccgttgaccg tcccgaatgg aagcggccgg atgatacgga
    27601 accgggaccc catcaggcgg tcttgatcat ggagatctcg gccatgtttg cccggaagag
    27661 ctcccgggaa tgggtggaga ttttctcgaa ggcggattgt tgttgcgagc tcgtgctaaa
    27721 cctccgggag gtcatggacg atccgcaggt tcgagcaaga cggatggtgg tggaggtcgt
    27781 tcaccaaagt tggagggcct accggcagcg ggggattgct cccaagtttt ccgcaacacc
    27841 gggcagcatt cggacctgct cccccgaacg gggggagcat acggattcga ttctcgcgga
    27901 aatgggattg gagacccacg aaatcggagc cctgagaagc ggaggggtcg tctaacaggg
    27961 tgttgaaaaa gcaacaaata tgcgtgcttt attttcaggt ttgatg
//
LOCUS       DAOOVC010000043        27945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1157_length_27945_cov_6.086339, whole genome shotgun sequence.
ACCESSION   DAOOVC010000043 DAOOVC010000000
VERSION     DAOOVC010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 27945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27945
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1157_length_27945_cov_6.086339"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLM79_07750"
     CDS             <1..489
                     /locus_tag="PLM79_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86242.1"
                     /translation="GSRTVGEEQEFCVFRIGGQPFAVPASGLVKYRKVSSRQAKKILE
                     RGRATLGDFKDFWGNIRKGLVGSWKELPAKTLKNRCFLPIPRDRLGTVGDPVGTSVLL
                     LSNGTEHGLLLVDSTGVGMRKEEIRPVPGPEGRLGLIGLEAREAVEVLDVDWVLKAVR
                     IE"
     gene            768..2393
                     /locus_tag="PLM79_07755"
     CDS             768..2393
                     /locus_tag="PLM79_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV86243.1"
                     /translation="MTLEEVLQTYYSRLEEAQLYIQQGLWDGAADLLDRVLSEIEENE
                     LPEESKNFLKAQISAIKDGMGHSEGLKPDAITVQDPRHGDSDLGAGSFDYGRVLMDGQ
                     FYAEAVEEFKKAAIAGDHPLECWELSADCAQHLGRWEEAIRYYEIVYTYPNSSEELRH
                     RVLRKLTRCSQNLRRVGFLAANPGSLWPAGDRKHPVETSPSGVKEETLHPPIRSVSDS
                     SVHQLVGKRVVSFRDERGQSPAGARLSYRVGELLHVGMTSLVVELEREDTGERFAGQV
                     LNAPYRDELLPELLAGWIQRVQMLRSPYVAKVYDLALGGGLCLIVREHLPWSLADLMS
                     DGNPLPIPVAAFVAYRISEGLGDLHLHMEEDDQIGCLYHLDLRPSRVLLHPEKGAVKM
                     VNAGLWEVLQRAKLPATGMRRLPLSFLAYRAPEQFRPYLARRKPPVFTDLYLFGTLFY
                     EMLTGVRAFQASSYEEYEIQHCDQYPTPPKVWRTDIPEDMNDMIMKCLERDPMKRWRN
                     ATQLSLLFEKSFGDFIQASANPLVPFVRDRTHH"
     gene            2452..4428
                     /locus_tag="PLM79_07760"
     CDS             2452..4428
                     /locus_tag="PLM79_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOV86244.1"
                     /translation="MVVIGVDTGGTFTDLVYVRGKEWRVHKLLSTPGNPARAVLDGLD
                     RTAPDPSPRRIIHGSTVATNTILERKGARVALITNRGFEDVLEIGRQNRSRLYDLNYR
                     RQPPLVERGMRFGAPGRILCSGEEIEELSAGEAQGVVERVLESGAESVAVCLLFSYLD
                     PRHELLLGELLESTGIPVSLSHRILGEFREFERTSTTVLNAYVAPRMSRYVRTLRDSL
                     RTGDRLHIMQSNGGTISAETALDCPVRTILSGPAGGVVGALHLGRLAGYSKLITLDMG
                     GTSTDVSLIDDQLSWTTESVISEYPVRVPMIDIHTVGAGGGSIARIDPGGALRVGPRS
                     AGADPGPVCYGKGEAVTVTDANLYLGRIDPAGFLGGTMPLFPERLEPRFEQMAAQASL
                     TGVELAEGILAVANAVMERAIRVVSIERGYNPEEFTLLSFGGAGGLHAAFLARLLHIP
                     RVLVPRNPGILSALGMLLADVLKDYSLTVMLPEEGLCAAVLKERFLPLERRALEELAE
                     EGIQDGAVVLERYLDMRYRGQSYEIPVPFREFDRKGFHDLHRRIYGYCDPEKPVEVVN
                     VRLRAVGTVPKPVMEKADGRVETIPASAEAGTRQVVFDSVSHVARVLRRDTLLPGNRI
                     DGPTVVTEYSSTTVIPPGTWAMVDPWGNLLIQVR"
     gene            4634..6196
                     /locus_tag="PLM79_07765"
     CDS             4634..6196
                     /locus_tag="PLM79_07765"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOV86245.1"
                     /translation="MIHPVLLEVFKHKFSSIAEQMGVTLQRTAYSPNIKERRDFSCAL
                     FNHRGELIAQAAHIPVHLGSMPLSVESAVRSVVMSPGDMAALNDPFQGGTHLPDITLV
                     APVFADETSPAFYVANRAHHSDIGGMSPGSMPLAVSIFQEGVLIPPIRLVENGQWESN
                     ALKLLLRNVRTPAEREGDFAAQRMANLIGIRELLELVKTHGAETVRSYARGLLDYAEQ
                     RMRETIGKVPDGRYSFEDFMEDDGIDGSNIPIRVTVEVRGSRAVLDFTRSSPQVTGSI
                     NAVRAITVSAVLYVFRSLMEEEVPANAGCLRPIEILTTPGSIVDASFPCAVAGGNVET
                     SQRIVDVILGALAQALPELIPAASQGTMNNVSIGGMDERTGRPFAYYETLGGGMGASM
                     AGPGESAVHSHMTNTLNTPVEALEYAYPMLVREYSIRRGSGGKGKHAGGDGLVRELRL
                     LSDAEVTLLSERRRRVPYGLEGGQSGASGRNTVRRGGRTEERPGKFSERLSKGDVLRI
                     ETPGGGGWGGKS"
     gene            complement(6206..8413)
                     /locus_tag="PLM79_07770"
     CDS             complement(6206..8413)
                     /locus_tag="PLM79_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOV86246.1"
                     /translation="MKASPSDQRLSFRILGMDCGEEVAILKKEIGPLVGGESNLAFDL
                     LQNKMTVSGGQADPEAISRAVAQTGMRAVPWDGSCPAGACPVEEGLWQRHGRVFLCAS
                     SGVFLAAGFVLDAWGSGGILAAWKGAAGHPAETLWIQGLPYAASVISGAWFILPKAFY
                     ALRKLRPDMNLLMVIAVAGAAILGEWLEAASVSFLFSLALLLESWSVGRARSSIRSLM
                     ELSTPVARVRCPVDGDIEEKPVEQVPVGATVLVPPGEKIPLDGILLKGSTSVNQAPIT
                     GESMPVFKKPGDEVYAGTINGEGAIEFRSTRPSSDTALARIIHLVEEAQGRRARTEQW
                     VERFARYYTPAMMALAGLVALGPPLLTGLPWSPWFYQALVLLVIACPCSLVISTPVSI
                     VAGLTTAARHGLLIKGGVYLEVPAGIQVVAFDKTGTLTRGKPRVRDVIPMDDHTVGGL
                     LSYAAALESHSTHPLARAIVSEAQEKNLSVPRAEEFTLLPGQGAQGRIGDRTYWVGSH
                     RLLDQWDKDNPAFHSMATRLEDSGHSLVVMWCDDHICGLVSVADGVRPEARQAVRSLK
                     ELGVRRVVMITGDNQRTAESIAAVTEVDEAHAELLPEDKVRVVRELKERYGQVAMVGD
                     GVNDAPAMAEASLAIAMGTMGSDTAIETADIALMSDDLSRLPWLVKHSRRTLGVIRQN
                     IFLSLAVKGLFVGMAVGGLGTLWGAIAADMGVSLLVIFNGLRLLGPPLSKRTG"
     gene            complement(8410..12072)
                     /locus_tag="PLM79_07775"
     CDS             complement(8410..12072)
                     /locus_tag="PLM79_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HOV86247.1"
                     /translation="MTQPVNKRLLRISAFASLALLLLVAAMVVLPSFFNLDPIKKGVL
                     AGIEQKLRCRVSMASLDFHLFPRPRVIIRDGKMSWPEKLEGGFRSAQIDVHLIPLFLG
                     KVRIQKLHIFSPDFRLGITELPGPVPSDSETQQKPSPEVMLSSLSSVIPAEMTGITVL
                     VTDGSLAVSRNRLPPVQVLGIDATLSVAGATVDLEGRAWSNLSDYLSARLKLDLRGFK
                     GEGRIALTRFRPDVLADSWYPDMRDLLGDSRFHLTVDFQAESFQNFSARFQGSAPHIT
                     LERGDEKVVLVADSLSGNLSRQGGTFDLTLQQLKLSYPHGDLSGFLHVDGTQPLVQLQ
                     LDGVQTDAAAVRKVAVTVWPNERVVNKIFEIVREGTVPHIRFTAQGAEFRDLKKSENF
                     QIHGRMEQGRVYVPNVELDIRNVYGDVVISNGVLEGTNLSGKVGDSPGWDGGLIVGLV
                     RKGGSELPFFLDITIEADLSDLPPILRRVVKSDLFLRELELVRNPRGRALGRLVLGDT
                     LKSVQTRVVVENFELSGEYRRFPHPIETRGGTFFYEGTTAAVFGLQAVLGRSKIEEAS
                     VLFNSRPSPHLDVRSVRGAVAVEEVFPWLQSFKPVRTATRDLGSAHGTLLVESGNWKG
                     TLLHRENRLYHFQGRTENLLVKSPRLPDSLVLKKGRFEVVPDRVTLHQWELACSDALV
                     RVSGRVAGDFTGPARLDVTLDGSLGEKTVQWVHQALQVPGVLLPRPPVRLSQLRWLRE
                     TPDSQTFTGTLASNGCEVQLDLVREPRTFTVRRCAVRDSESAATLSFKREEQRSSFSF
                     AGHLTGNTLNGLLLQNPPLSGEVRGDFKADIHWDQPGRSTASGSLFVKGLNASRTPDS
                     PVIIHSMAMEGTRQGLALGSGSFRWRGQEMTMSGTVDFQSDAYLLNLDAAADAIQWQA
                     LREAEKTPESASGGEPQGKSTSVPLKGVIRIRASSLSYEKYTWSPFGAEVTLLPDGYD
                     IALTHSVLCGIPTPGNIRFGRDGLQVDLKPAAKGAALESSLACLWEKDRLITGSFGLR
                     GEIKGQGGGRALTESLEGVLSFNANEGRIYRLEILSKILALLNVTEIFRGRMPDLFRE
                     GFAYDSIHAKAHFDHGKLLLDESVIDGSSMKIVSRGEVDLVRKKLDLSVLVAPLKTAD
                     NLLQYMPLLNHMFEGGLISIPFQVTGDLSNPDVRPMPASGTGAELMNLMKRTLRAPLK
                     VIEPFLPQTKEGAEPS"
     gene            12327..12680
                     /gene="ssrA"
                     /locus_tag="PLM79_07780"
     tmRNA           12327..12680
                     /gene="ssrA"
                     /locus_tag="PLM79_07780"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            13537..13770
                     /locus_tag="PLM79_07785"
     CDS             13537..13770
                     /locus_tag="PLM79_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV86248.1"
                     /translation="MDKQHSMARLVRELRKLTGLTQEKFSARLGVTFPTINRWENERA
                     KPSPLALEKIESLLRSFGDKGEALHRANFGKEA"
     gene            13773..13985
                     /locus_tag="PLM79_07790"
     CDS             13773..13985
                     /locus_tag="PLM79_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86249.1"
                     /translation="MARPEFIEAFEERLWSAEDTLRANSNYAGNENFLPVMGLAFLRI
                     SSATGLFPASCGSSIAPSRKRTATRC"
     gene            14051..14275
                     /locus_tag="PLM79_07795"
     CDS             14051..14275
                     /locus_tag="PLM79_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86250.1"
                     /translation="MNLLGYCSMDDEGTFKALVRMFEQALKAIATLEPNQQGAYVERL
                     ERIRREGHNRGRGVGDDMDDLMAEHGFAGK"
     gene            14582..15052
                     /locus_tag="PLM79_07800"
     CDS             14582..15052
                     /locus_tag="PLM79_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86251.1"
                     /translation="MKCGNCDQRRLLPVADQVIYDRPAGKQTIGVYPLLGDDSRCFPA
                     ADFEKADWREDAGAFLQSCGELGIPAVPKISRSGNGAHIRISFAEPVPAREARQLGAA
                     LISHTCDRTRQFIIRELRPPVPQPGHRAQGRLREPYCALPAEAAEGTGPQCVRR"
     gene            complement(15902..16072)
                     /locus_tag="PLM79_07805"
     CDS             complement(15902..16072)
                     /locus_tag="PLM79_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86252.1"
                     /translation="MAGFDKAIPPGAEGKISIKLNPKQCLGGARKVTLVLTNDPQTPK
                     FTLVVQGKLRDQ"
     gene            complement(16082..16342)
                     /locus_tag="PLM79_07810"
     CDS             complement(16082..16342)
                     /locus_tag="PLM79_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86253.1"
                     /translation="MQRRLFKNILLLVVVAGACFHILGPAQEAAKPDTAFASEAAGKP
                     ALEVPEWYFDFGEVKDGSEYLHAFVIRNAGTGVLEIKKVQPG"
     gene            complement(16465..17583)
                     /locus_tag="PLM79_07815"
     CDS             complement(16465..17583)
                     /locus_tag="PLM79_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV86254.1"
                     /translation="MDRVDVVIVGAGPGGVSAAIRSRELGLKAVVLEKGLRLFQGIIE
                     TYPRGKKVYPTIPKDEEQPFAIPDLEPANEPVEEYVSRIEACVAKHGITICYGEEFQG
                     FSREKDSLTVTTVKNRYKTSAVILAFGSNIPIDLGIYGEAKVVARNLGNPEEHIGVPT
                     LVLGGGNAAADVVSTLSKTKRAAGDSTPVYWGHRREHFKIDKDVARDLGEEILLGGNI
                     KILQGAVPRIGEVDEDGVERLIIQTQRFALPHGVEITHGMSFPMKHVIACIGTQGPSS
                     IFDRLGLQQITCTAGVCKIGKEGAKLLMLSHGLRTSVNGVYAIGGAISPAYLHVEEQG
                     TLRERKHSNLIFKAVQDGVRAVEDIAMQRGGTAPPRPG"
     gene            complement(18049..19281)
                     /locus_tag="PLM79_07820"
     CDS             complement(18049..19281)
                     /locus_tag="PLM79_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOV86255.1"
                     /translation="MIPNTGVSAFRTIMAAWALWACALLCSAARGEEPPEKALAVIGS
                     ARITARDLENRIERLSPVLREEYSSEEGRQRLLREMVRIEVFSMEARARGLDRAPEFQ
                     RKLAEVEKALLADHFAKEAVLPGSEGTEEEAWEYYARHLEEFQSPEKIRVSSVFLECP
                     RSAPPDERARRRLLAEEILQRARNGEDFPALARKHSSKSPDEEAGYFSRGRYLPEIEE
                     RIFALNPGELGPILETEGGLWLFRVEDRLPPAEQPYGAVRAEILARLRMEKGLRVFDD
                     LENRLFAKYAVKFPGEDSPASQEGTASRKEGGSPAMEGTITTVTRAAPEIREAGGRGS
                     IFVEAGPGAAPGMDRASIGITSKTQISRKTGGRLREATAHDLKKGQRVRIFISGPVGS
                     SYPVQADADSVVILEDPP"
     gene            complement(19326..20390)
                     /locus_tag="PLM79_07825"
     CDS             complement(19326..20390)
                     /locus_tag="PLM79_07825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HOV86256.1"
                     /translation="MGNLLSILMIFALVAIPSLPGAQGGEMTVSQSVEPKAAVQAANY
                     WTRERVLNARPHPMGIPGRAVTGTREDAGIEEPGAVPGSPGGKSVLLSEDPADTLSLT
                     GAFESGSAILNDIHVLGYSYPPPHTTFYVPTTLYGTTASTYPYRVIGKVFFTKSDGKN
                     YVCSGSSIGGRAVLTAGHCVSDGKGAFHTNWVFIPAYRDNTRPYGTWSAFWKTTFTVW
                     HKYSDYGRDVGFAAVSDISGTKLSQRVGTLGFAWNQSTIKHWNMFGYPAASPYDGARM
                     VQTQASLSRVDESKSPGRPGIGTTQTPGCSGGPWILNFITSNYANGVNSSFFTTAPQE
                     MFSPYFDTAVNNVRIAAIAK"
     gene            21411..22331
                     /locus_tag="PLM79_07830"
     CDS             21411..22331
                     /locus_tag="PLM79_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOV86257.1"
                     /translation="MTNLILWFKETRPQFLLLSVVLTFLGTSIAWYYGSFNPGYALLA
                     GFGLLLTHGSVNAINDYFDYKSGIDLEVVRTPFSGGSGLVPEGKLPLKQALWVGVITS
                     LAALVIGIVFVIVSGWLLVPLLVVAILCMVLYTPVILRTYWPEWSPGLGLGILPVLGF
                     YFVQTGRYDWVVLVASVPSGILVHNLLLLNEFPDVDADRKGGRKTTPVVFGMEAAGKF
                     YRIATVSVYVWIVGCVAATVMTGKVVMPVYCLIAFLALPFALKAMRGSREYHDRNKLV
                     AALGHNVAFILLTQVLLGVGYLLDKAFPIF"
     gene            complement(22509..22805)
                     /locus_tag="PLM79_07835"
     CDS             complement(22509..22805)
                     /locus_tag="PLM79_07835"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOV86258.1"
                     /translation="MGRTRTKGEEFHYAVFRAYFIDGLNLARVPVLVNLAESIGLEGA
                     EDVLAQGTFNESVDEEWRCSRECGIAAVPTFMANGRRLVGPQPYQAPEKSTKTD"
     gene            23388..23939
                     /locus_tag="PLM79_07840"
     CDS             23388..23939
                     /locus_tag="PLM79_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOV86259.1"
                     /translation="MKPIGPMMWEHRLIEKMLRLFEGEIRKINESNKLDTVFLDMAVD
                     FIRTYADRTHHGKEEDILFRDLAKKPMSPDHARTMNELVEEHKSSRQIVGKLVEAKER
                     YLEGEDTLQEVVSLLGELARFYPLHIEKEDKHFFYPCMEYFTREEQDKMLAEFYEFDR
                     TMIHEKYRKVVERMEARYTVSQA"
     gene            24463..26295
                     /locus_tag="PLM79_07845"
     CDS             24463..26295
                     /locus_tag="PLM79_07845"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOV86260.1"
                     /translation="MKLGLEKVLVVLCAAVVYALAPGSPLCSSALAEDYRFEFLWPTL
                     QKGGYFCFPYAVVTDRNGNVYIADCYNHRIQKFSRDGRFLKEWGSYGSAPGRFRYPQG
                     VATDGLGNVYVADTDNHRVQKFTPEGQFLKEWGGYGTGDGQFIGPFGMAADPNGNVYV
                     ADTGNDRIQKFTSDGQFLTQWGETGTEDGQFNSPCGITVGLGGSVYVADAGNDRIQKF
                     TSDGQFAAKWGASGTARGRFNSPLGIAVDRGGNVYVADTGNDRIQKFTSDGQFVAKWG
                     KRGNRKKRFNVPAGIGVGPDGTVYVADTDNHRIQKFTSDGKFVGQWKSWAGGDGYFRR
                     PMGVALDTVGDVYVADSGNRRVQKFAPDGQYLTQWGTKGKEDGQFRVPCHTATDRDGN
                     VYVVDSRNCRIQKFTAEGKFLTAWGTMGGEPGQFFYPYGIAVGRAGNVYVTDNWLQRV
                     QKFTSDGEYLTGWGTMGMGEGEFALPTGVAVDNNGSVYVADSLNGRIQKFTSSGKYVA
                     QWGNQGSGGAKLSLPEGVAVDDEGGVYVSDAEANCIRKYTADGKFLTRWGGGGSDPGK
                     FNFPAGIAVDGRGRVYVADSMNHRVQVFKKLPKEAQARDARDDR"
     gene            26285..27364
                     /locus_tag="PLM79_07850"
     CDS             26285..27364
                     /locus_tag="PLM79_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86261.1"
                     /translation="MIDRKHAKTMGPGDEKIPVKIDYDIVVEPGQEFTVDRFRPEDAR
                     GIAHLFFSEYGAGYPVETYYFPERIIEENRNGNLYSVMARTPRGDIVGHMALYRSSPP
                     YPKLYEIGQGIVLPSYRTTFAAYKINRYIGETLAEEVRPDGIFGEAVCHITATQKSSA
                     LIGMKPVALEIDLMPAEAYDKAQCASSRVSCVVLFRSFNDRPREVFIPPVYGREIEFI
                     LEDLDLERTLTSSREEIPRDTESHISVRFFPDAGVGRFTVLSAGWDFPGRVDHLEEQG
                     AMNGTRIFQYFLNLAEPWAGRAVECLRERGYFLGGYVPRWFDADGLLMQKLGTVPDFA
                     AIKLHSEKAKRIRDIVCSDRQRAGS"
BASE COUNT         5410 a         8392 c         8698 g         5445 t
ORIGIN      
        1 gggtcccgga cggtcggcga ggaacaggag ttttgtgtct tcaggatcgg gggacaaccc
       61 tttgctgtcc cggcttccgg actggtcaag taccggaagg tttcctcgag acaggccaaa
      121 aagattctcg aacgaggtcg cgccaccctg ggggacttca aggacttctg gggcaatatc
      181 cgaaaggggt tggtgggaag ctggaaggaa ctcccggcca agaccctcaa gaaccgttgt
      241 tttctcccca tcccccggga tcggctggga accgtgggag atcccgtggg cacatcggtc
      301 ctcctcctca gcaacggcac ggaacatgga ctgctcctgg tggattccac gggcgtcgga
      361 atgaggaagg aggaaatccg tccggtcccg ggacccgagg ggcgactggg gctgattggc
      421 ctcgaagccc gggaagccgt cgaggtcttg gacgtggatt gggttctcaa agccgtccgg
      481 atcgaatagg ataggcaacg ggcaacccag gatgaacccc gggccgggct atgggttggg
      541 ggcgtgaggc gaggggccgg gaccgagatt gagcggtctg accggtagga atcccgtttc
      601 gtcattccct cgcatgcggg aagccggaaa gagcgtgcat acccgccgga tgcccgcacg
      661 cgcggacatg acgccccgat gctttcggaa tccctcgact cgaggcggca ggttcccgcc
      721 gtgctcgggg cgggatgctc agtcgggagg aggagtgaca cgggcacatg actcttgaag
      781 aggtgcttca aacctattac agtcgactcg aggaggcgca actctatatc cagcagggcc
      841 tttgggatgg ggctgcggac ctgctggatc gcgtgctttc ggagatcgaa gagaacgagc
      901 ttcccgaaga gagcaagaat ttcctgaagg ctcaaatcag cgccatcaag gacgggatgg
      961 ggcacagcga aggactcaag cccgacgcta tcaccgtcca ggaccccagg catggggact
     1021 cggacctcgg ggccggctcc ttcgactatg gtcgagtgct catggacggg cagttctatg
     1081 cagaggccgt cgaggaattc aagaaagccg ccatagcggg ggaccatccc ctggaatgct
     1141 gggaactgag tgcggactgt gcccagcacc tggggcggtg ggaggaggcc atccgctatt
     1201 acgaaatcgt ctatacctat ccgaactcct ccgaggaact gaggcaccgg gtgttgcgaa
     1261 agctcacgag gtgttcgcag aatcttcgaa gggtcggatt cctggcggca aacccgggaa
     1321 gcctttggcc cgccggggac cgaaagcatc cggtcgagac aagcccgtcg ggagtgaagg
     1381 aggagaccct tcatccgccc attcggtccg tcagcgattc ctccgtccac cagctggtgg
     1441 gaaagagggt tgtctccttc cgcgacgaac gaggacaaag cccggccggg gctcgacttt
     1501 cctatcgcgt aggggaattg ctccatgtgg gcatgacctc gttggtggtg gagttggagc
     1561 gggaagatac gggggagcga tttgccggcc aggtcctcaa tgctccctat cgggacgaac
     1621 tgctccccga attgcttgct ggatggattc agagagtgca gatgctgcgg tctccttacg
     1681 tggcaaaggt ctacgacctg gccctgggag gcggcctgtg cctcatcgtt cgggaacatc
     1741 tcccatggtc cctggcggac ctgatgtccg acggaaaccc cctgccgatt cccgtagcgg
     1801 ccttcgtggc ctaccggatt tccgaggggc tgggagacct tcatctccac atggaggagg
     1861 acgatcagat aggatgcctg tatcacctgg atttgcgtcc atcgagggtc ctcctgcacc
     1921 ccgagaaggg ggccgtgaag atggtcaatg caggcttgtg ggaggtgttg cagagggcca
     1981 agctgccggc caccggcatg cgccgacttc ccctctcctt tcttgcctac cgggccccgg
     2041 aacagttcag accctacctc gcccgcagga agccccccgt cttcaccgac ctttacctct
     2101 tcgggaccct cttctacgag atgctcacgg gggtccgggc ctttcaggct tcttcttacg
     2161 aagagtacga gatccagcat tgcgatcagt acccgacccc tcccaaggtc tggaggacgg
     2221 acatccccga ggacatgaac gacatgatca tgaagtgcct ggaacgcgat cccatgaagc
     2281 gctggcgaaa cgccacccag ctttccctgc tcttcgagaa gtccttcggg gatttcatcc
     2341 aagcctccgc caatcccctg gttccgttcg tccgggaccg aactcaccac tgaaccgaag
     2401 tcgctccagg atgcgcaaaa cggtccgaca tttcccggac gggggggaac catggtcgtc
     2461 atcggggtag acacgggggg gacgtttacc gacctggtat acgtccgggg gaaagaatgg
     2521 agggtgcaca agctcctttc cacccccggg aatccagccc gggccgtgct cgacggcctg
     2581 gaccgcacgg ccccggaccc atccccccgg cgaatcattc acggctccac cgtggccacc
     2641 aacaccatcc tcgaacgcaa gggggcccgg gtggccctca tcaccaaccg gggattcgaa
     2701 gacgtcctcg agatcggaag acaaaaccgc agccggttgt acgacctgaa ctaccgaaga
     2761 cagccccccc tggtggagag aggaatgcgc ttcggcgcac cgggccgaat tttgtgctcg
     2821 ggtgaagaga tcgaggagct ttccgccggg gaagcacaag gggttgtgga acgggtcctc
     2881 gagtcggggg cggaatccgt ggcggtctgc ctgctttttt cctacctgga cccccggcac
     2941 gagttgctcc tgggagaact cctggaatcc acggggattc ccgtttctct ttctcaccgg
     3001 atcctggggg aattcaggga attcgaacgc acctccacca cggtcctcaa cgcgtacgtg
     3061 gctccccgga tgtcgagata tgtgcgaacc ctgagggatt ctctgcggac gggggaccgt
     3121 ctccacatca tgcagtccaa cgggggcacc atttccgcgg aaaccgcgct ggactgcccc
     3181 gttcgcacca ttctttcggg gcccgcgggt ggagtcgtgg gggccctcca cttgggacgt
     3241 ctggccgggt actcgaaact gatcaccctg gacatgggag ggacctcgac ggacgtctcc
     3301 ctcatcgacg atcaattgtc ctggaccacc gaatccgtca tttccgaata cccggtgagg
     3361 gttcccatga tcgatatcca caccgtggga gccgggggcg gttccattgc ccggatcgat
     3421 cccggggggg cactccgggt cggtcctaga agtgcgggag cggatccggg gcctgtctgc
     3481 tacggcaaag gggaagccgt caccgtcacc gacgccaacc tctatctggg gcggatcgat
     3541 cctgccgggt tcctgggcgg aaccatgccg ctttttcccg aacgactcga gccccgcttc
     3601 gagcagatgg cggcccaagc atccctcacc ggggtcgagc tggccgaggg cattctggcc
     3661 gtggccaacg ccgtcatgga acgggccatt agggtggttt ccatcgaacg aggctacaac
     3721 cccgaggaat ttactctcct ctccttcggg ggagccgggg ggctgcacgc cgcctttctg
     3781 gcccgcctcc tccatatccc tcgagtcctg gttccccgca atccgggaat cctctccgcc
     3841 ttgggaatgc tcctggccga tgtcctcaag gactactccc tcacggtcat gcttcccgaa
     3901 gagggtcttt gcgccgcagt cttgaaagaa agatttctac ccctggaacg cagggccctc
     3961 gaggagctcg ccgaggaagg catccaagac ggagccgtgg tgctcgaacg gtatctggac
     4021 atgcggtacc ggggacagtc ctacgaaatt ccggttccct tccgggagtt cgaccgaaaa
     4081 gggtttcacg accttcaccg gaggatctac gggtattgcg atcccgaaaa gccggtggag
     4141 gtggtcaatg tgcgtctaag agccgtgggg acggtcccca agcctgtgat ggagaaggca
     4201 gacgggcggg tggaaacgat tcccgcctcg gcggaggcgg ggacccggca ggtggtgttc
     4261 gactccgtct cccatgtcgc tcgggtcctc cggcgggata cactgctgcc gggaaaccgc
     4321 atcgacgggc ctaccgtggt cacggaatat tcctccacca cggtgattcc cccgggaacc
     4381 tgggcaatgg tggacccgtg gggcaacctc ctcatccagg tccgttgagg gccggggcgg
     4441 tgttccgggc cccgcggcgg cgcggagccc accccccacc agatgggacc ccggcaacgc
     4501 cgcccccgcg gcggcccacg agaatcgccc aaagcccccg gcattcggct ttggcaacgc
     4561 cgccccctgg ggcggcgcaa cgcccggccg cttggcttca gaccgccgca accccttcct
     4621 ttcggaggtt cttatgatcc atcccgtcct gctggaagtg ttcaagcaca agttctcctc
     4681 catcgcggaa cagatggggg tgaccctcca gcgaacggcc tattccccca atatcaagga
     4741 acgaagggat ttctcctgtg cgcttttcaa ccaccgggga gagctcattg cccaggcggc
     4801 ccatatcccc gttcatctgg ggtccatgcc cctgtccgtg gagtccgccg ttcggagcgt
     4861 tgtcatgtct cccggggaca tggcggcctt gaacgacccc ttccaggggg ggactcacct
     4921 gcccgacatc accttggtgg cccccgtgtt tgcggacgag acttctcccg cgttctatgt
     4981 ggccaatcga gcccaccact cggacatcgg gggaatgagc cccgggtcca tgcccctggc
     5041 cgtttccatc ttccaggaag gtgttctcat tcctcccatc cgcctcgtgg aaaacgggca
     5101 atgggaatcg aacgccctga aacttctcct gagaaacgtg cgaacccccg ccgaaaggga
     5161 gggggatttc gctgcccagc gcatggccaa cctcatcggg attcgagagc tcctggaact
     5221 ggtgaaaacc cacggtgcgg aaaccgtgcg gagctatgcc cgggggttgc tggattacgc
     5281 ggaacaacgc atgcgggaga ccatcgggaa ggtccccgac ggtcgctatt cgttcgagga
     5341 tttcatggaa gacgacggga tcgacggctc caacattccc atccgggtca ccgtcgaggt
     5401 ccgaggaagc cgcgccgttc tggacttcac ccgatcgagt ccccaggtga ccggaagcat
     5461 caacgcggtt cgagccatta ccgtttcggc ggtgctctac gtgttccgat ccctcatgga
     5521 agaggaggtc ccggccaatg cgggatgcct gcgtcccatt gaaatcctca cgaccccggg
     5581 cagtatcgtg gacgcgtcct ttccctgtgc cgtggccggg ggaaacgtcg agacttccca
     5641 gaggatcgtg gacgtgatcc tgggggccct tgcccaggcc ctgccggagc tcattcccgc
     5701 cgcaagccag ggaaccatga acaacgtcag cattgggggc atggacgagc gaacggggcg
     5761 accctttgcc tactacgaga ccctgggcgg aggcatgggg gcctccatgg ccggtcccgg
     5821 ggaaagcgcc gtccattccc acatgaccaa taccctcaac actccggtgg aggccctcga
     5881 atacgcgtac ccgatgttgg tccgggaata ttccatccgg agaggttcgg gggggaaggg
     5941 caaacacgcg gggggagacg gactggtgag ggagctccga ctgctgtccg atgccgaggt
     6001 gaccctcctg tccgagaggc gacggcgggt gccgtacggc ctcgaggggg ggcaatccgg
     6061 ggcctcgggc cgcaatacgg tgcggcgagg cgggcggacc gaggaacgcc cgggaaagtt
     6121 cagcgaacgg ttatccaagg gggatgtctt gcgcatcgaa acccccggag ggggcggatg
     6181 ggggggaaaa tcctgaatcg gcgtctcagc cggtccgttt cgacaaagga ggccccaaca
     6241 gtctcaggcc gttgaagatc accagcaggg agacccccat gtccgcggcg atggctcccc
     6301 acagggtccc cagccctccc acggccatgc ccacaaacag gcctttgacc gccaaggaca
     6361 ggaagatgtt ctggcggatg acgcccaggg tccgccgaga gtgcttcacc agccagggaa
     6421 ggcgggagag gtcgtcggac atgagagcga tgtccgccgt ctcgatggcg gtgtcgcttc
     6481 ccatggttcc catggcgatg gccagggagg cttccgccat tgccggagcg tcgttgactc
     6541 cgtctcccac catggccact tgcccgtacc gctccttgag ttcccgcacc acgcgaacct
     6601 tgtcctcggg caacagttcg gcatgagcct cgtccacttc ggttacggcc gcgatggatt
     6661 cggcggtcct ttgattgtcc cccgtgatca tgaccacccg gcgaaccccc aactccttga
     6721 gggatcgaac ggcctgcctc gcttcgggcc tcaccccgtc cgcaacgctc acgagtccgc
     6781 agatgtggtc gtcacaccac atgaccacca gggaatggcc cgaatcctcg aggcgggtgg
     6841 ccatggaatg aaaggccggg ttgtccttgt cccactgatc caggaggcgg tggctcccta
     6901 cccaataggt gcgatccccg attcttccct gggctccctg gccgggcaac agggtgaatt
     6961 cctccgcccg tggaacggaa aggtttttct cctgcgcctc ggagacaatg gcccgggcca
     7021 gggggtgagt gctgtgggat tccagggcgg cggcgtagga aaggagccct cccaccgtgt
     7081 ggtcgtccat gggaatcacg tcccggaccc tgggctttcc tcgagtgagg gttcccgtct
     7141 tgtcgaaggc gaccacttga atccccgctg ggacctcgag atatacgccc cctttgatga
     7201 ggagcccgtg gcgtgccgcc gtggtgagac ccgccacgat gctgaccggg gtcgagatga
     7261 ccagggagca gggacaggca atgacgagca gcaccagggc ctggtagaac cacggagacc
     7321 acgggagccc cgtgagaagg ggagggccca gggccaccag gcccgccagg gccatcatgg
     7381 ccggggtgta gtaccgggcg aaccgctcga cccattgctc ggtgcgggcc cgcctgccct
     7441 gggcctcctc caccaggtgg atgattcgag ccagggcggt gtccgaggaa ggcctcgtgg
     7501 aacgaaattc gatggctccc tccccgttga tggtccccgc atacacctcg tcccccggct
     7561 ttttgaaaac cggcatggat tcccccgtga tgggggcttg attgaccgag gtggaaccct
     7621 tgaggaggat cccgtccagg ggaatcttct cccccggggg gaccagtacc gtggctccca
     7681 cgggcacttg ctccacgggc ttttcctcga tgtctccgtc cacgggacaa cggacgcggg
     7741 ccacgggggt cgatagctcc atgagactcc tgatgctgct gcgggcccga cccacgctcc
     7801 aggattccag gaggagggcg agagaaaaga ggaagctcac cgatgcggct tcgagccatt
     7861 ctcccaggat cgcggctccc gccaccgcga tgaccatgag aaggttcatg tcggggcgaa
     7921 gctttcgcag ggcgtagaag gccttgggaa gaatgaacca ggccccggag atcaccgacg
     7981 cggcataggg aagaccctgg atccacaggg tttctgccgg gtgccctgcc gctcccttcc
     8041 aggcggcgag gattcctccg ctcccccagg cgtccaggac aaacccggcg gccaggaaca
     8101 ctccgctcga ggcgcacaag aatacccggc cgtgtcgctg ccacagaccc tcttccacgg
     8161 ggcatgcgcc cgccgggcag ctcccgtccc atggaaccgc ccgcatgccc gtttgagcca
     8221 cggcccggga aatagcttcc gggtccgcct gccccccgct gacggtcatc ttgttttgca
     8281 ggaggtcgaa ggctaggttg gattcccctc ccaccagggg accgatctct ttcttgagaa
     8341 tcgcgacttc ctcgccgcaa tccatgccca ggattcgaaa ggaaagtcgt tggtccgagg
     8401 gagaagcttt catgaaggtt cggccccctc tttggtttgg ggaagaaaag gctcgatcac
     8461 cttgaggggg gcccggaggg tccgtttcat gaggttcatc agttcggctc ccgttccgga
     8521 tgcgggcatg gggcgaacgt ccgggttcga gaggtccccg gtaacctgga aaggaatgga
     8581 aatgaggccg ccctcgaaca tgtggttcag caaaggcatg tattgcagca ggttgtcggc
     8641 ggtcttgagg ggagccacca agacggaaag atccagcttc ttgcgaacca ggtccacctc
     8701 cccccgggag acgatcttca tggaagatcc gtcgatgacg gattcgtcca ggaggagctt
     8761 tccatggtcg aagtgggcct tggcatggat ggaatcatac gcgaagcctt cccggaacag
     8821 gtccggcatg cgaccccgga agatctccgt gacgttcagg agggcaagga tcttggacag
     8881 gatttccaac cggtagatgc ggccctcgtt ggcgttgaaa ctgaggaccc cctcgaggga
     8941 ctccgtcaag gcccggcccc ctccctgtcc cttgatttcc ccccgcaggc cgaaggaccc
     9001 ggtgatgagg cggtcttttt cccaaaggca cgccagtgag ctttccagtg ccgctccctt
     9061 ggcggcgggc ttcaggtcga cttgcagccc gtcccggcca aaccggatgt tccccggggt
     9121 gggaatgccg cacaagaccg agtgggtcag cgcaatgtcg tacccgtcgg gcaacagggt
     9181 cacctccgcg ccgaaagggc tccaagtgta cttttcgtaa gacaaggaag aggcccggat
     9241 tcgaatcacc cccttcaagg gaacggaagt ggattttccc tggggttccc ctccggaggc
     9301 gctctccggg gttttctcgg cctccctcaa ggcctgccat tggatcgcgt cggcagccgc
     9361 atccaggttc aaaaggtacg cgtcggactg gaaatccacc gttccgctca tggtcatctc
     9421 ctggcctctc cagcggaagg agccggaacc cagggccagc ccctgccggg tgccttccat
     9481 ggccatgctg tgaattataa ccggagagtc gggagtccgg gaggcgttga gccccttgac
     9541 gaacaggctt ccggacgccg tggaccggcc cggctgatcc cagtggatgt cggctttgaa
     9601 atctccccgg acctcccccg agaggggagg attctgcagg aggagcccgt tgagggtgtt
     9661 cccggtaaga tgcccggcaa aggagaagga agatcgttgt tcttcccgct tgaagctgag
     9721 ggttgccgcc gattccgaat cccggaccgc acatcggcgg acggtgaagg ttcgaggttc
     9781 ccgaacaaga tccagctgaa cctcgcaccc gttggaggca agggtcccgg taaaggtctg
     9841 ggaatccggg gtttcccgaa gccatcgcaa ctgggagagt cgaacggggg gacgaggaag
     9901 aaggacccca gggacctgga gggcctggtg cacccactgg acggtctttt ccccaaggga
     9961 cccgtccagg gtcacatcca gccgggcagg cccggtgaaa tcgcccgcaa cccggcccga
    10021 gacgcgcaca agggcgtccg agcacgccag ctcccactgg tggagggtca ctcgatcggg
    10081 aaccacttcg aaccgaccct tcttcaagac cagggagtcc ggcagccggg gggattttac
    10141 cagcagattc tcggtcctcc cctgaaaatg atagaggcgg ttctcccgat gaagaagggt
    10201 tcccttccag ttgcccgatt ccacaaggag ggtcccgtgg gcggaaccca ggtcccgggt
    10261 tgccgtccga acgggcttga acgactgcag ccagggaaag acctcttcca cggcaacggc
    10321 gccccgcacg gaccggacgt ccaggtgagg ggacgggcgg ctgttgaaaa ggaccgaggc
    10381 ctcctcgatt ttcgatcgcc ccagcaccgc ctgcagtccg aagaccgcgg cggtggtgcc
    10441 ttcgtagaaa aaggtgccgc cacgggtttc gatgggatgg ggaaaacgcc ggtattcccc
    10501 cgaaagctcg aagttctcga cgaccacccg ggtttgaacg gacttgaggg tgtctcccag
    10561 gaccagtctt cccagggctc gcccccgggg gttccggacc agttcgagct cgcgaaggaa
    10621 gaggtcgctc ttcaccaccc ggcggaggat cgggggaaga tcgctcaaat cggcctcgat
    10681 ggtgatgtcc aggaaaaagg gcagctcgga gcctcccttg cgaaccagtc ccacgatgag
    10741 gcctccgtcc cagccgggag agtcccccac ttttcccgaa agattggttc cctcgaggac
    10801 tccgttggag atgaccacgt ccccgtacac gttccgaatg tccagttcca cgttgggcac
    10861 atacacccgg ccctgttcca ttctcccgtg aatctgaaag ttctccgact ttttcaagtc
    10921 gcggaattcg gccccctggg ccgtaaagcg gatatgaggc accgtcccct cgcgaacgat
    10981 ctcgaagatc ttgttcacca cccgctcgtt gggccacacg gtgacggcaa cctttcgaac
    11041 ggccgccgca tcggtctgaa caccgtcgag ctggagctgc accaggggtt gggtcccgtc
    11101 cacgtggaga aatcccgaaa ggtccccgtg gggataactg agcttgagct gctgcagggt
    11161 gaggtcgaag gtcccgcctt ggcggctcag gttcccgctg agggaatccg ccacgaggac
    11221 gaccttctcg tcgccccgct cgagggtaat atggggagcg gacccttgaa accgggccga
    11281 aaagttttga aaagattccg cctggaaatc aacggtcagg tggaaccggg aatcccccaa
    11341 caggtcccgc atgtccggat accagctgtc cgccagaacg tccggccgaa agcgggtcag
    11401 ggcaatccgg ccctctcctt tgaatcctct gagatccaac ttgagacggg cggaaagata
    11461 atccgaaagg ttggaccagg cccggccctc gaggtccacc gtcgccccgg cgacggagag
    11521 cgttgcgtcg attcccagca cctgcacggg agggaggcgg tttcgggaca cggccaggga
    11581 accgtccgtc accaggacgg tgatgccggt catctcggcg gggatgaccg aagagaggga
    11641 agaaagcatc acttccgggc tgggtttttg ttgagtttcg gaatccgagg gcacggggcc
    11701 gggaagttcc gtgatgccca ggcggaaatc gggggagaag atgtggagct tttgaatccg
    11761 gacttttccc aggaacaggg ggatgagatg cacatcaatc tgggcggaac gaaaacctcc
    11821 ttccagcttt tccggccagg acattttccc gtcgcgaatg atcacccggg gccggggaaa
    11881 gaggtgaaaa tcgagggaag ccatgctcac ccggcatcgc agtttctgtt cgatcccggc
    11941 gagaactccc ttcttgatgg gatccaggtt gaaaaagctg ggaagaacga ccatggccgc
    12001 caccagcaga agcagggcca aggaggcgaa ggcggaaatc cgcagaaggc gtttgttcac
    12061 cggttgtgtc atgcacccaa cattcttgag aagcccgggc tgtaagcgac cgatccccca
    12121 tgggtccttt ggtcttggaa ggtctgccca ccgaacgttc taggatagca attggcgaag
    12181 gggcgggcaa gagtttcggc cacgccgaat gctcccggcc gccggggaaa ggagcagggg
    12241 ggaaagccga gccaggaagc ccggcgcttg acttcgaatc tcttcggggt acaatgcaat
    12301 tagtcgagca cagggcttta ccgaatgggg gcgaaacggg ttcgacggag gtaaagaagt
    12361 tcaagctgca tgccgaggtt ccgactcctc gtaaaaccgt tggagccaaa acaaacgcag
    12421 acgactatgc gtacgccgta gccgcctagg cggctaccgt ctctccggtt ctctcctgcg
    12481 ggaccgatag aggcgtcgac tcagcgggat ggtccttgac cccgcgccta cggtttcaag
    12541 gacgagattc gataggctag ccgaggcaat atcccgcccg cggggagctt gccgaggcga
    12601 aaatcaaaac ccgggataag catgtagatg cttgggcgga atgctttcgg acgcgggttc
    12661 gactcccgcc gcctccacca ttcgtctaag gctgggagcc ggtttgcggg ctgccaagcg
    12721 cacacgcatg tcgtgcatcg gggtcccacg aaccattcta cgaaatttcg ggttgttttc
    12781 gagtcgttcg cggggtcgca gatcgcctta accgcgcccg gtggtgaggc ggcttttctt
    12841 tttcggcgta ttgtcagaga cttacgtctg ttttggacta cctgaccttc cgacctaaac
    12901 tctcctcttt cgaagaatcc gtcctacccc gcttctcttt gggcccaaat tctccgaact
    12961 cctaccccga ttctccgggc ctcgtccggt gtcctcacac taccctgcgt ccgcggttcg
    13021 gcggcatcaa aggcaaaccg ccggatcctc ttcgtgattc cgtcaaacag gtcgttgatt
    13081 cccatggacg gtgcctcccc ctgtgggtga tgcccctcct tgccggagag acggagaaag
    13141 tgtcagccgg tggctgaaat tctttcgctc tgatgtctgt ggaactcacc tgccgggtgg
    13201 cggcgggatg tgtcgaagaa tacttgaggg tgactcttgt ctcacgggga tttccgtaag
    13261 gattgcaaag taatgcgtga catgtgcggc cctgtggatg agatacggca agtgatcaat
    13321 catgcgttgg cgccgcctgc gacgtcgcca atgcggcgtt gcccccatcg ggtccctgtc
    13381 ccggccggct ccgcctccgg ctctgccaac caggccattg gacccgatgt cactcgcttg
    13441 catgcgcctt cggctccgca agcttcgcgc cacgggtcaa cgccgcatta tgaatggtgc
    13501 cgccggcaaa tcagaaattc agcaaggaga aatccattgg acaagcaaca cagtatggcc
    13561 aggctggtcc gggaacttcg gaaactcacg ggtctgactc aggaaaagtt ctccgcccgg
    13621 ctcggggtga cgttccccac gatcaaccgc tgggagaacg agcgcgccaa gccgtcgccg
    13681 ctggcgctgg aaaagatcga aagcctgctg cgcagcttcg gggacaaggg cgaggccctg
    13741 cacagagcga attttggaaa ggaagcttga gcatggcccg gcctgaattc atcgaagcct
    13801 ttgaagagcg gctctggagc gcggaggaca ccctgcgggc caactccaac tacgccggca
    13861 acgaaaattt cctgccagtg atgggcttgg ccttcctgcg gatctcttct gcaaccggac
    13921 tgttccctgc gagctgcggt tcctcaatcg cgccaagccg gaagcgcacc gcgacaaggt
    13981 gctgatgatc gatgcgcgca acatctaccg caaggtcacg cggatgacct agttcttttg
    14041 cgaaccctgt atgaacctgc tcggctactg tagcatggat gacgagggta ccttcaaggc
    14101 cctggtgcgt atgtttgagc aggcgctgaa ggcgattgcg actcttgagc ctaatcagca
    14161 aggagcctat gtcgaacggc ttgaacgcat ccggcgcgaa ggccacaacc ggggccgggg
    14221 cgtcggcgat gacatggatg atctcatggc ggagcatgga tttgccggaa agtgacaaga
    14281 tcttgaatga acaagacgga ctgcggcggt tgcgtacgga gaacgcccgc ctcaaggaat
    14341 tgctgaccca tcaggacacg gcctgggaag caccgaccgc gcctgggacc gttccggctc
    14401 catcggagcc tcctcgagtc ccacccattt caccaccgac gacaagatcg ccctgttccg
    14461 ttgcctgttc cggggtcgcg aggatgttta tccgaagcgc cgggagtcgg ccaaaggcac
    14521 atccggctat tcaccgacct gtggtaacga gtggaagccc ggcgtctgcc acaagccccg
    14581 tgtgaaatgc ggcaactgcg accagcgccg gttgctgccg gtggccgatc aggtgatcta
    14641 cgaccgtccg gccggaaagc agaccatcgg cgtctatccg ctcttgggcg atgacagccg
    14701 ttgttttccc gcggcagact tcgagaaagc cgactggcgg gaggatgccg gagccttcct
    14761 gcaatcctgc ggggaactcg gcattccggc ggttccgaag atttcccgct ccggaaatgg
    14821 ggcacacatc cggatctctt ttgccgagcc ggttccggcc cgcgaggccc ggcagctcgg
    14881 ggccgccttg atcagccata cctgcgaccg cacccggcaa tttatcattc gcgagctacg
    14941 accgcctgtt cctcaaccag gacaccgcgc ccaaggacgg cttcgggaac cttattgcgc
    15001 tctccctgcg gaagcagccg agggaactgg gccgcagtgt gttcgtcgat aagcacctgc
    15061 aaccctattc atgccaagtt gcgcccaagg tcgctctcaa ctgctgagcg aaactttcct
    15121 gcgcagggct aaatcctgcg gatgggcagt ttcggcttga gtgtgatcag ccccagctcg
    15181 cccgtatgtg gaacaatctt cggcgcggct accgggccat gggctatgag atcaaaccga
    15241 tgaagaccgt tgtcttggga aacggcctca agtaagacga gccgtgccaa acggcgcctt
    15301 cctccgggca aggggcagga agcgtccggc ggaaaccgca tggggaagca cggccgttgc
    15361 cataccgatc gggcacgaac aggcttggca aggcctgagc ccgggcaggc ggagatcgcg
    15421 gatgatctct aaacgtggaa gtgagtagga ggcggacaga gcataccgtc cgcccggcag
    15481 gccctcggga gactccacaa gggaattgag ggaagcgccc ccgatggaat ttgtggcgaa
    15541 acctattcct agtgattaaa cgggacttgc agcaggaagg gttccgaaga agtcaacggg
    15601 aacccaaagg agcgaaagat ggatgccgga agcaaagaag aatatggatc caagggagtg
    15661 gttgagttgc gttgcgactc cgctcgatgt tggaagtgcg acgaaaatgc gtgtaaagag
    15721 cggttcgaag agctggtttt cgggattggg ccgtaagctc ctcgatcccc atagagagtc
    15781 ttgagaattc cctgtgtgag ccgcttcctt acactgcggg caaaagggta atccgagaga
    15841 cctcggcgaa agccggggtc tcctggtttg gggaggttgc cgttcctgcc tgccgacctg
    15901 tttactggtc tctcaatttc ccctggacca ccaaggtgaa tttgggtgtt tgaggatcgt
    15961 tggtcagcac aagggttact ttccgggcac cgcccaggca ttgtttcgga ttgagcttga
    16021 tactgatctt cccctcggcc cccgggggaa tggccttgtc gaaaccggcc accgaagatc
    16081 ctcaccccgg ctggactttc ttgatctcca ggaccccggt tcctgcattt cggatgacga
    16141 atgcgtgaag gtactcggac ccatccttca cttctccgaa atcgaaatac cattcgggga
    16201 cttccaaagc gggttttccg gccgcttccg aggcaaacgc cgtatccggt tttgcggctt
    16261 cttgtgcggg ccccaaaatg tggaagcatg cccccgcgac cacaacaagc aataggatgt
    16321 ttttgaaaag tcttcgttgc attgttgccc ccatgggagt tgggtacatc ctgttccgat
    16381 ctacatcgcc ccctgtcctg gaatttcaaa gacctggatg gagcgatgag gccccgcgcc
    16441 gcccgtacaa gaccggttcg cctgtcagcc cggccgaggc ggtgcggtac cgcccctttg
    16501 cattgcgatg tcttccaccg cccgaacgcc gtcctgtact gccttgaaga tcagattgga
    16561 atgtttcctt tcccggagag tgccctgttc ctcgacatgc agatatgccg ggctaatggc
    16621 tcctccgatg gcatacaccc cgttcacgct tgtccggagg ccatgactga gcatgaggag
    16681 ctttgcgcct tccttcccga tcttgcagac ccctgccgta cacgtgatct gctgcaggcc
    16741 gaggcggtcg aaaatactcg agggcccctg ggtcccgatg caggcgatca cgtgtttcat
    16801 gggaaagctc atcccatggg ttatctcgac tccgtggggg agtgcaaacc tctgggtctg
    16861 gatgatgagc cgctcgaccc cgtcttcatc cacctcaccg atacgcggca cggccccttg
    16921 caggatcttg atgtttccac ccagaaggat ttcttccccg aggtcccggg cgacgtcctt
    16981 gtcgattttg aaatgttctc gacggtggcc ccagtagacg ggcgtggagt cgcccgcggc
    17041 ccgtttggtc ttggataggg tggagaccac gtcggcggcc gcgttgccgc cccccagaac
    17101 cagtgtcggc actccgatgt gctcctccgg attgccgagg tttcgggcca ccaccttcgc
    17161 ttccccgtag atgcccagat cgatggggat attgctcccg aatgccagga tcacggccga
    17221 agtcttgtag cggttcttga ccgtggtcac ggtcaaggag tccttctccc gtgagaagcc
    17281 ctggaattct tctccataac aaattgtgat gccgtgtttc gccacacaag cctctatccg
    17341 ggaaacatat tcctccacgg gttcgtttgc cggttcgagg tccgggatgg cgaagggttg
    17401 ctcctcatcc ttgggaatgg tgggatagac cttcttgccc cggggatagg tctcgatgat
    17461 cccctgaaag agtcgcagcc ctttttccag cacaacggct ttcaacccca gctcgcggct
    17521 cctgattgcg gcagaaacgc ctcccggccc cgcacccacg atgacaacat ccactctgtc
    17581 catctctcgc agcctccagt tagccaacct tgtcgacaag cagggcttgt cactatcgct
    17641 caagtccgat ccatgcacaa ggggcaatca cgacggacac ggcgaccaag agcttcttgc
    17701 gagacctcga gtcgaggggc tcgggacgga cccagactcg gattcagacc cgagtggatg
    17761 gggtggccgt ggggtatgcc cagggccccg ggactcgagg gtctcgacct gtactgccgc
    17821 cccgggaagg agttccagag tgtcgcgttc ggaactcaag ggcacgtcct cgggggtgtt
    17881 ggaagggggt cgttcttggg gcggaaatat ggattcgcga tttggaaagc gggtttcttt
    17941 cggcccgtga gggaattggg ttcggggcgt tgcatccccg attcggttgc ccggcctttt
    18001 tccccgtcca caatcccaac cgccaagctc gtcttgctcg atggaggtct acggggggtc
    18061 ttcgagaatc acaacggaat ccgcatccgc ctgaacgggg taggacgacc ctaccggtcc
    18121 ggagatgaag atccgcactc tctgcccttt cttcaggtcg tgggcggttg cttcccgaag
    18181 ccgccctccc gtcttcctcg agatctgcgt cttcgaggta atgccgatgg aggcccggtc
    18241 catgcccggg gcggctcccg gtccggcttc cacaaagatc gatccgcggc ctcccgcctc
    18301 ccgtatctct ggggcggcgc gggtcaccgt ggtgatggtt ccctccatgg ccggagatcc
    18361 cccctccttt ctcgatgcag tcccttcctg cgatgcgggc gaatcctcac cgggaaactt
    18421 tactgcatat ttggcaaaaa gccgattctc gaggtcgtcg aaaacccgga ggcccttttc
    18481 cattcgaagc cgggcaagaa tctctgcgcg aacggccccg taaggttgtt cggccggggg
    18541 caggcggtcc tcgactctga agagccaaag gccgccttcc gtttcgagga ttgggccgag
    18601 ctctcccggg ttcagcgcaa agatccgttc ttcgatctcc ggaaggtatc gaccccggga
    18661 gaaatacccc gcctcctcgt cgggcgattt cgacgaatgc tttcgggcca gtgccggaaa
    18721 atcttccccg tttcgggctc tttggagaat ctcttcggcc aagagccgcc ttcgggcccg
    18781 ttcgtccggg ggcgcggaac gaggacactc gagaaacacc gaagagaccc ggatcttttc
    18841 cggcgattga aattcctcga ggtgccgggc atagtattcc cacgcctctt cctcggtgcc
    18901 ctcagacccg ggaaggaccg cctccttggc aaaatgatcc gccagcagcg ccttctcgac
    18961 ttccgcgagc ttccgttgga attccggcgc ccggtccagt cctcgggccc gggcttccat
    19021 ggaaaagacc tcgatgcgaa ccatttccct caggaggcgt tgccttcctt cttcggacga
    19081 gtactcctcc cgcaggaccg gggagagccg ctcgatccgg ttttccaggt ctcgggcggt
    19141 gatccgggcg gacccgatga cggccagcgc cttttccggg ggttcttccc ctcgtgccgc
    19201 gctgcacaac aaggcgcagg cccatagagc ccacgccgcc atgatcgtgc gaaaggcgga
    19261 aacacccgtg ttgggaatca tgacctcgat cctccccggg ccgaaggcgg tggtgttcac
    19321 ccccgctact tggcgatggc ggcaatccga acgttgttca cggccgtgtc gaagtacggg
    19381 gagaacattt cctggggggc cgtggtgaag aagctgctgt tcaccccgtt ggcatagttg
    19441 gacgtgatga aattcaagat ccacggaccg ccgctgcagc ccggcgtctg ggtcgtaccg
    19501 atgcccggcc tgccgggaga tttggattca tcaaccctgg acagggatgc ctgggtctga
    19561 accattcgcg ccccatcgta tggggaggcc gcggggtagc cgaacatatt ccagtgtttg
    19621 atggtggatt ggttccacgc aaatcccaat gtgcccactc tttgggaaag cttcgtcccg
    19681 gaaatgtcgg acaccgccgc gaacccaacg tctcgcccgt agtcgctgta cttgtgccag
    19741 acggtgaagg tggtcttcca gaaggcggac catgtgccgt agggccttgt gttgtcccta
    19801 taggcgggga tgaacaccca gttggtgtga aaggcaccct tgccgtcgga cacgcaatga
    19861 ccggcggtca ataccgctcg ccccccgatg gaggacccgg agcatacgta gtttttcccg
    19921 tcgctcttgg tgaagaagac tttcccgatc acccggtagg ggtacgtaga ggcggttgtt
    19981 ccgtagagag tggtgggaac atagaaggtc gtgtggggag gagggtagct gtacccgaga
    20041 acatggatgt cgttcaggat cgcgctcccg gattcgaatg ccccggtaag agacagggtg
    20101 tcggccgggt cctccgagag gagcacggac tttcccccgg ggctgccggg aaccgctccg
    20161 ggttcctcga tgcctgcgtc ctccctagtc cccgtcaccg cccgaccggg gattcccatg
    20221 ggatgaggcc gggcgttcag gaccctctct cgggtccagt agtttgcggc ctgcacagcc
    20281 gccttaggct cgacgctctg ggataccgtc atttcccccc cctgagcacc gggtagggac
    20341 gggatcgcga ccagggcgaa gatcatcaga atgctcagca gatttcccat tgctttcctc
    20401 ctctgggcgg gccgggcagc gtcccgccga attgggggtc cttaaaaagc gttcttcccg
    20461 ccgaccaatc cctgacggag ccacaaaaac gagccgtaca accactcggc atgttcacac
    20521 aaaggcctct gtccgggcct ctctcaagga tttccgcgcc tgttccggac gggagccgca
    20581 ttttccggga tgagacccgg ctttgatcct tctcgagtcc ggcccccgac gtcaggggca
    20641 caggtgcggc aaccgattct ttactatccc ccgtggcctc gagaaccccc atcacggaaa
    20701 ccgctcctcg acgatcggcc gagaacattt cgatgcttgg gggggcgcca ccgccctggt
    20761 ggcgaaacga attcggtccg ttgaagttgt tggtcccagt gagcgaatca gagtcgccgg
    20821 ttccaggaaa accggcagcc cggtcgatcc gttgcgccgc gtccttgggg gtatcggcgc
    20881 cgcgatcctt ggatatctta tcctccggct cctggtgcag gatgagaacg aggcgataaa
    20941 ggggtcataa gaaattgctt ttaccgaggt ttcaccttat cagccgtcgc tgttcctgtc
    21001 aattccgatc tttggccgga ttccatgcac ggaatgaccg ggcccaacga ggttttgcgc
    21061 acgaagggtg tgagatctaa gcaaatcttc atgcaggggt gtatcatggg taccggaccc
    21121 gtagaattgc ccaaagccca tccgtgaatg agaagcggcg gatgccggaa cccggggtgc
    21181 actctacggg aaaccgacac gggagaagaa tcgagcgctc gcactttgct cctgacaccc
    21241 ggtcctttgt cgtgtatgga atgcttggac gaggatgttt gaatcgccgg gaacggttcc
    21301 cggggcgcgg ggagcctccg gcacgccgtg gacccgggag atccgcactg ggagtcgatc
    21361 ccctctcgac ccaatgcggt cccatttcgc tgatcggtgg aggagattcc gtgacaaacc
    21421 tgatcctgtg gttcaaggaa accagacctc aattccttct actttccgtc gttctcacct
    21481 ttctggggac ctccatcgcc tggtactacg gttctttcaa cccggggtac gcattactgg
    21541 ccggttttgg actgctgttg actcacggta gcgtgaacgc catcaacgac tatttcgatt
    21601 acaagagcgg gatcgacctg gaggtcgttc ggaccccatt cagcggagga agcgggctgg
    21661 tccccgaggg aaaactcccc ctgaagcagg ccctctgggt tggggtaatc acctccctgg
    21721 cagccctggt catcgggatc gttttcgtga tcgtgagcgg atggcttctg gttccgctcc
    21781 tggtggtggc gattctgtgc atggttctgt atacgcccgt gatattaaga acttattggc
    21841 cggagtggtc tccgggcttg gggttgggga tcctgcccgt cctgggtttc tattttgtgc
    21901 agacgggccg atacgattgg gtggtcctgg ttgcctcggt acccagcggg atcctggtcc
    21961 acaatctact cctgctgaac gagtttcccg acgtggacgc cgatcgaaag ggtgggcgga
    22021 agacgacccc cgtggtgttc ggcatggaag ccgccgggaa gttctaccgg atcgcgacgg
    22081 tatccgtcta tgtctggatc gtcgggtgcg tggcggcaac cgtcatgacc gggaaagtgg
    22141 tgatgcccgt ctattgcctg attgcgtttc tggccctgcc cttcgccctg aaagcgatgc
    22201 ggggaagcag ggaataccac gatcggaaca aactggtggc ggccctcggc cacaacgtgg
    22261 cgtttatcct gctgacccag gtcctgctgg gggtgggtta cctcctggac aaggcgtttc
    22321 cgatattctg acgggaagga cggggctcct aagggagcga atgaactgct gtcgacggtc
    22381 ggtgagtccg ccctgtcggc accacgggtg tgagtttgtt tccgtcactg gggccgaagg
    22441 ggtctgtcca tcccttttcc aggatttcgg tccgggtgga aatgatccaa ggggatgacg
    22501 gatcggcatc agtcggtttt ggttgatttt tccggggcct ggtaaggctg agggccgacc
    22561 aacctgcgtc cgttggccat aaaggtcgga accgccgcaa tcccgcactc tcgggagcat
    22621 ctccattctt cgtccaccga ttcattgaag gttccttggg cgaggacgtc ttcggctccc
    22681 tcgaggccga tggattccgc taggttcacc aaaaccggga cccgggcaag gttgagtccg
    22741 tcgataaaat aggccctgaa gacggcgtag tgaaattctt ctcccttggt ccgggttcgg
    22801 cccatttgcc cacttcctgt gcccgccggg tgttgtaggt cttttctcgg gttcccaggg
    22861 ggaggccgca gtcgctcgcc gcctttttga gccggttcaa cctggacgga atatccacgt
    22921 ccctcccggc gaaaagctct gccggggtga cctcttccgg cgtttcgggg cgcaaggata
    22981 atgcgatcca cttgatctcg agatcgtgtg cattcgtgag ttgttcaata cgccgggtac
    23041 tgaaacggca gcaaggtccg gtgtcgtccg agaagatctt catgaggatt cgatcggcca
    23101 tcattccgtt ccttgtctgc cgtctcaaga tagttcccct gcccgatcct ttgtagagtg
    23161 tcatcagagc cggaatccgt ggtgtgaaaa accggaatct ttcaatagaa cgggatgggt
    23221 cccgacgatg ggtcttgcca aacctgggga tgagctttaa gtaccggggt acatgcctgc
    23281 gcaatgaggc ctgcatttgc cgagagccga gggcttccaa ccgcgaagtc ccccttgtcg
    23341 agctctcaac cacccgtaac cacaaccggg aaaggataaa cgttgccatg aaacccattg
    23401 gcccgatgat gtgggagcac cggctcatcg aaaaaatgct tcgtcttttt gaaggcgaaa
    23461 tcaggaagat caacgaatcc aacaagctgg acacggtttt tctggacatg gccgtggatt
    23521 tcatccggac ctatgcggat cggacccatc acggcaaaga agaagacatc ctgttccggg
    23581 atcttgcaaa aaagccgatg tccccggacc atgcccggac catgaacgag ctggtggagg
    23641 aacacaaatc ctcgcgacaa atcgtgggaa aactggtgga agccaaggag cggtatctcg
    23701 agggcgagga caccctgcag gaggtcgtct cccttctcgg ggagctcgcc cggttctacc
    23761 cgctgcacat cgaaaaggag gacaagcact tcttctatcc ctgcatggaa tatttcaccc
    23821 gggaggagca ggataagatg cttgccgagt tttatgaatt cgaccgaacg atgattcatg
    23881 agaagtatcg gaaagtggtc gaacggatgg aggctcggta taccgtttcg caagcctgag
    23941 cataagtctg cattcgcaaa agggcttgtt ttcgtacccc cggataaatc cgggagcccc
    24001 gcctcctgcg ttccttgtcg gcaggtacca atcgattccc atttttgccg ggatgcttcc
    24061 cctgctccct ttgctcgatg tctggaaaag cgacgggact tcccccgggg caaagctcgt
    24121 gaagggtttc cgccccgggg gatcgtcggc ggggacaagc ttcggattgc ggaggcgttc
    24181 ggtttatggt aaaggtccgg tatcacgaag ttatcaacac gagggggaga aggtgcggag
    24241 ggaccggacc tcgggaccga gttccggtgg aaggagaccg cgggaaagac ggacggccgt
    24301 ccgagacggg cgcatccggt cttgacccga cccgccatcc cgacagggct ttcccttgat
    24361 cccgggcgtc gcccggtgta ttcgcttcgt tttccggcac aggatgagga actgccgatc
    24421 tccggctccg gtttcccacc gacagcggaa ggaggacatc ccatgaagct cggtctcgaa
    24481 aaggtcctcg tggtgctctg cgccgcagtg gtgtatgccc ttgctcccgg gtcccctctt
    24541 tgttcttcgg ccctggcgga ggattaccgt ttcgagtttc tgtggccgac cctgcaaaag
    24601 ggcgggtatt tctgtttccc ttatgccgtc gtaacggacc gcaacgggaa cgtgtatatc
    24661 gcagactgtt acaaccatcg cattcagaag ttctcgcggg atgggcggtt cttgaaggaa
    24721 tggggaagct acgggagtgc tcccgggcgt tttcggtatc ctcaaggcgt cgcaacggac
    24781 ggcctcggga acgtatacgt ggcggacacg gacaaccacc gtgtccagaa gttcacgccg
    24841 gaagggcagt tcttaaagga atggggagga tacggcacgg gggacgggca gttcatcggt
    24901 cccttcggca tggcggcgga ccccaatggg aacgtgtacg tggcggatac cggcaacgac
    24961 cgaatccaga agttcacctc ggacgggcag ttcttgaccc aatggggaga aaccggcacg
    25021 gaagacgggc agttcaactc cccctgcggc atcaccgtag gcctcggggg cagtgtatac
    25081 gtggcggatg ccggcaacga ccggatccaa aagttcacct cggacgggca gtttgccgcc
    25141 aagtggggag catccggcac tgcccggggt cgattcaact ctcccttggg catcgccgtt
    25201 gaccgaggtg ggaacgtgta cgtggcggat accggcaacg accgaatcca gaagttcacc
    25261 tcggacgggc agtttgtcgc caagtggggc aagcggggga atcgaaagaa gcgttttaac
    25321 gttcccgcgg gcataggggt gggtcctgac ggcacggtgt atgtggcgga taccgacaat
    25381 catcgtatcc agaagttcac atccgatgga aagttcgtgg ggcaatggaa gagctgggcc
    25441 ggtggtgacg ggtattttcg acggcccatg ggggtggccc tcgacaccgt cggcgacgtg
    25501 tacgtggccg attccggaaa ccgccgcgtc cagaaattcg ccccggacgg gcagtatctc
    25561 acccagtggg gaaccaaggg gaaggaagac ggacagttcc gcgtgccctg tcacacggcg
    25621 acggaccggg acgggaatgt gtacgtggtg gattcccgga actgccgcat ccagaaattc
    25681 acggcggaag ggaagttcct caccgcgtgg ggaaccatgg ggggagagcc cggccagttc
    25741 ttctatccgt acggcatcgc ggtgggcagg gccggcaatg tctacgtgac ggacaactgg
    25801 cttcaaagag tccagaagtt cacatcggac ggggaatatc tcaccggttg gggaaccatg
    25861 ggaatgggcg agggagagtt tgccctcccc accggagtgg cggtggacaa taacggcagc
    25921 gtgtacgtgg cggattcttt gaacggccgg atccagaagt tcacatcgag tgggaagtat
    25981 gtcgcccagt ggggcaatca ggggagcggc ggagcgaagt tgagtcttcc cgaaggcgtg
    26041 gcggtggacg acgagggcgg ggtctatgtg tcggatgcgg aagccaattg catccggaaa
    26101 tacaccgcgg acgggaagtt cctcacccgg tggggaggcg ggggaagtga ccccgggaag
    26161 ttcaactttc cggcaggcat cgcggtggac ggccggggca gggtgtacgt ggcggattcc
    26221 atgaaccatc gggtccaggt gttcaagaag ctccccaagg aagcccaagc ccgggacgca
    26281 cgggatgatc gataggaagc atgcgaaaac catgggaccg ggagatgaaa aaattcccgt
    26341 gaaaatcgac tacgatattg tcgtggaacc cggacaggag ttcaccgtcg accgttttcg
    26401 gccggaagac gcccggggga tcgcccactt gttcttttcc gagtacggag ccgggtatcc
    26461 cgttgaaacc tattactttc ccgagagaat catcgaggaa aaccgaaacg gcaatctgta
    26521 ttccgtgatg gcccgcactc cccggggaga catcgtgggt catatggccc tctaccgcag
    26581 ctccccccct tatcccaagc tctacgagat cggtcaggga atcgtcctgc ccagctaccg
    26641 caccaccttt gccgcctaca agatcaaccg gtatatcggg gagacgctgg cggaggaggt
    26701 gcgcccggac ggaatcttcg gcgaggcggt ttgtcacatc accgccaccc agaagtcatc
    26761 ggccctcatt ggaatgaaac ccgtggcgct ggaaatcgat ctgatgcctg ccgaggccta
    26821 cgataaggcc cagtgcgcct cgagccgggt ctcctgcgtg gtcctgtttc gttcctttaa
    26881 cgaccggccc cgggaggtct tcattccccc cgtctatggc cgggaaatcg aattcatcct
    26941 ggaagacctg gatctcgaac ggaccctaac gtcttcccgg gaagaaattc cccgggacac
    27001 ggaaagtcac atctccgttc gtttcttccc ggatgcggga gtggggcgct tcaccgttct
    27061 ttccgccgga tgggatttcc ccgggcgggt cgaccacctc gaggaacaag gggcgatgaa
    27121 cgggacccgg atattccagt attttctcaa cctggcggaa ccctgggcgg ggcgggcggt
    27181 cgagtgcctc cgagaaagag ggtacttcct cggaggctat gtaccccgat ggtttgacgc
    27241 agacggcctc ctgatgcaga agctcggaac cgtccccgac tttgccgcca tcaagctgca
    27301 ctcggaaaag gcaaaaagga tccgggacat cgtttgctcc gaccggcaac gggccgggtc
    27361 ctaactccga tctacttcat cttcccgttt aagaaagccg cgaagccgtt gatcgtcttg
    27421 agcaccgata cgtcggcatc cgacaggttc acgccgtact tcttctcggc tgcggcggcc
    27481 acggccggca gatcgatgga atcaatcccc tgggagcagg ggtacgtcgt acttcaagtc
    27541 tttgatgatc tggtcgtcaa tcccacttcc ttcatcaagc cgatgatctc ttccgcgccg
    27601 accgccatga tgctctcctc tctggtggtt catacacaca cgggtccttt cctccacagg
    27661 cgatcctgtg caccgggatc caaccggcaa acaggcccct cactctttcc tcgaccccga
    27721 ccgattgcgg tggacttgcc gtcaccccgg cgaactcggg gtcctgtccg cgacgggact
    27781 tgggggtcgg gcatcctccg gacgcttgcc ggggatttcc tcacaaaaat tgaattgcac
    27841 cggacacatg tgcttgggaa gcctctttag cacatgagag cgaagatccc gggccagctc
    27901 cggggtgcct tccgggccgg gattcagtac cacatgggcg atggg
//
LOCUS       DAOOVC010000044        27327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1190_length_27327_cov_7.436235, whole genome shotgun sequence.
ACCESSION   DAOOVC010000044 DAOOVC010000000
VERSION     DAOOVC010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 27327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27327
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1190_length_27327_cov_7.436235"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            487..2187
                     /locus_tag="PLM79_07855"
     CDS             487..2187
                     /locus_tag="PLM79_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86262.1"
                     /translation="MKWLKSLSLLDFTDKGDSPERYALLRRNILILMVIVTIFPLCFM
                     ALVNYHQYQKALREEIVNPLRTLVNKTKHSFELFLAERLSTLSLIASAYSFDQLSDEN
                     TLTRIFAAMEREFRGFVDLGLINSAGEQVSYAGPYDLRGKDYSEQGWFHEVQVRGSYV
                     SDVFMGYRLFPHVAIAVQRRGPDGRVWILRATINTEKFDDLISSMGLEPSSDAFLLNK
                     SGVFQTPSKHFGNVLDPFPLQLPPITYEANVIEEKTPAGKGVLMAYTYFVNAPYILAV
                     VKPQGVILKAWYTLKSELFFLFLGSVVGTLLVIFKLTDVLVRRMRECDEKREAAFREM
                     QHTHKLSSIGRLAAGVAHEINNPMAIINEKAGLMKDLMEFIPQFPEREKFASLTDAIL
                     QSVHRCRSVTHRLLGFAKRMEVQIEELDLNETIHETLTFLEKEALYRNLDLRLNWGAD
                     LPRIVSDRGQLQQVALNLLNNSFAAVEDGGVVEVTTWRKDARTVALSVRDNGCGMSPE
                     TLRHIFEPFFTTKKDSGTGLGLPITYGIIKKLGGDIEVQSREGEGTTVSVFLPVQMQP
                     "
     gene            2204..2596
                     /locus_tag="PLM79_07860"
     CDS             2204..2596
                     /locus_tag="PLM79_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86263.1"
                     /translation="MQKWKVLLVDDEKEFVSALSERLMLRGLDVRIAFSGEEALGSVQ
                     SDPPQVVVLDVMMPGLGGAEVFRRIRELHPNIPVIFLTGQGCSVDRRQTVPYGAFECL
                     VKPLDIDDLIRTISSAIGLCETPALLSE"
     gene            2621..4756
                     /locus_tag="PLM79_07865"
     CDS             2621..4756
                     /locus_tag="PLM79_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOV86264.1"
                     /translation="MHRFFPFLSWVKTIDFTTFRLDFLSGITVALILIPQSMAYAQLA
                     GMPVHYGLYASLLPPMLAALFGSSRQLATGPVAIVSLMTSAALAPLATAGSDSYVAYA
                     ILLALVVGIFQFALGVLKLGLVVNFLSHPVVNGFTNAGALIIASSQLSKLFDVYVDEA
                     EHHYETVYRVVQAALAYTHRPTLFLGALAFGIMYGLKRIDRRIPNVLIAVVVTTLISW
                     LTGFEHNRTVPLEAVGSEEVRQLIVGFNEEVRAVDHLAAKRAALTPEIESAAKQYGEN
                     SPQVLELRHKVMLMTAKIQDLRASSGDKRSRLRTCLFQGIQEPGKALMLVPRETVSEG
                     TKTDGRTWRIKVGNRPLDESKLLFMGGGSVVGEIPKGLPALEVPRIDLGVMIHLLPIA
                     AIISLLGFMEAISIAKAMAAKTGQRLDPNQELIGQGVANMLGAVGKSFPVSGSFSRSA
                     VNLQSGAVTGLSSVVTSTTVGIVLLFFTPLLYHLPQSVLAAVIMMAVVGLLNVSGFIH
                     AWKAQWYDGVFSVLTFVATLLSAPHLDKGIMLGVVLSLGMFLYKSMRPRVSTLSMYAD
                     QSYRSASHYGLRECKHIALVRFEGQLFYANAGHLEESISNLLMAKKELKHIVLVASGI
                     NDMDATGEEMLSLVVDRLRSMGYDMSLCGVNENVLGVMKRTHLLAKIGEENVFPTIPA
                     AVNAIHARAHTQCRLDHCPLQSIRFVEND"
     gene            4801..6033
                     /locus_tag="PLM79_07870"
     CDS             4801..6033
                     /locus_tag="PLM79_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86265.1"
                     /translation="MPVIAMMFGSFCHGEEVAGKVAAELEYSLLKDDFFFARAAGNFR
                     MDEGKLRKAVTGKTSVFNQFTHERERALAYLKATLAESLTGDNLLLLGFLAHLIPPEI
                     SHVLKVCLIADIRRRVREARRVEGISEKDALKRIHKDDEGIVLWTEYLFQRKDPWAPD
                     LYDILLPMGKDSVDRAAAVICENVRKQVLMVSDQSRRGVEDFILASRVNVKLAEEGHD
                     VQVSARDGHVRVTINKKVLRLSALEEELKKLALSVPSVRSVETRVGAGYYQTDIYRKY
                     EFDVPLPTKVLLVDDEREFVETLSERLRMREVSSAVAYNGREALDVVDADEPEVMVLD
                     LKMPGIDGLEVLRKVRDEHPDVEVIVLTGHGSEEIRRICMEMGACAYLEKPVEMENLT
                     AAMKEAYDRVKQRKARQR"
     gene            6284..6952
                     /locus_tag="PLM79_07875"
     CDS             6284..6952
                     /locus_tag="PLM79_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86266.1"
                     /translation="MEDYRNQELAFLGKMAAGFTHEVKNVLAIIRENAGLIEDILTMA
                     PAEAVPNLDRVLRALSAIRDQSGRGVELSNRLNRFAHCADQPRAHIELNETLAQFTLL
                     VERFARLKEVTLTLHRGESGIEMETRPVGLQLALFGVLECCWNSVSSLRRLDLKVESE
                     PNQGVVEIVGKGEDDRPVGFHETPETVDDWARVVSRLDALGAKVERGPAVDVLRIVFP
                     LRSP"
     gene            6976..7392
                     /locus_tag="PLM79_07880"
     CDS             6976..7392
                     /locus_tag="PLM79_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86267.1"
                     /translation="MTDLDVLLVDDEKEFVAALAERLAMRCLKTRIAFSGEEALELVR
                     QKVPDVVVLDLKMPGMNGLDVLRLLKKSHPQVPVIMLTAHGSERDREAGRLLGAFDQL
                     QKPVEIGALIRSMKEACGHRRTRPEADNGVPPRPVQ"
     gene            7599..7970
                     /locus_tag="PLM79_07885"
     CDS             7599..7970
                     /locus_tag="PLM79_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86268.1"
                     /translation="MGIRLLLVDDEEDFSRTVAERLTLRGMNVRIASSGEDALQRMEE
                     SPPDVVVLDVVMPGMGGLEILRWIKNRHPSIQVILLSGFASSQDARKGMTMGAFDYLL
                     KPVDLEELVRKILDGYRAMER"
     gene            8754..11168
                     /gene="gyrB"
                     /locus_tag="PLM79_07890"
     CDS             8754..11168
                     /gene="gyrB"
                     /locus_tag="PLM79_07890"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOV86269.1"
                     /translation="MPSLEGTDKAATDQYTAQQITVLEGLSAVRMRPSMYIGNVAVEG
                     LHHLVYEVVDNSIDEALAGFCDHIRVQIHLDGSVTVDDNGRGIPVDTHEKENLPAVEV
                     VMTKLHAGGKFDDSTYKVSGGLHGVGVSVVNALSEFLDVEIRREGKVYSQSYARGDAV
                     TPLRVTGETSRRGTRITFKPDPLIFSDTELSFDILAQRFRELAFLNPGVSIHLLDERI
                     PREREFCYQGGLVSFVEYLNKNKEPLHPEIIFISGDRQGISVEIAIQYNQTYNEKIFT
                     FANNIHTKEGGTHLIGFKAGLTRAIKQYAVQTKMAKSDLDKLTGDDVREGLTAVLSVK
                     LSQPQFEGQTKTKLGNSEVKGLVENLTYEKLSAFFEENPKVIRSILDKVLEAARAREA
                     ARRAKELTRRKGVLSDHSLPGKLADCQERDPSKSEIFIVEGDSAGGSAKQGRDRRFQA
                     ILPLRGKILNVEKARFDKMLENQEIRTMITALGTGIGKDEYDPDKLRYHKTIIMTDAD
                     VDGAHIRTLLLTFFFRQMPDMIERGHLFIAQPPLYRVAAGKHEQYLKDEDELNAYLLK
                     RAVEKKSVTLPALDSPIPPEQLIQLLKTYSRYEEWLERQEQKGMTKALLDLAIRIFSR
                     NKLVPSHEESMSVLRMELEKAGYEVESMELEEEHRGYDLEVWQPTNGQQRIRLTYDFL
                     QSIEFRKLLELYDQLEVLHQTPYTVKDANKEVRYDDPRALFHSLMDEARKGLTIQRYK
                     GLGEMNPEQLWDTTMNPEKRTLLQVRIEDQYQADELFTTLMGDRVEPRRDFIQVNALD
                     FRELDI"
     gene            11213..13645
                     /gene="gyrA"
                     /locus_tag="PLM79_07895"
     CDS             11213..13645
                     /gene="gyrA"
                     /locus_tag="PLM79_07895"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOV86270.1"
                     /translation="MDARTINIEDEIKLSYLDYAMSVIIGRAIPDVRDGLKPVHRRTL
                     FSMHELGNDYNKAYKKSARIVGDVIGKYHPHGESAVYDALVRMAQDFSMRYTLVDGQG
                     NFGSIDGDPPAAMRYTEVRMSRLAGEFLRDIEKETVPFQPNYDNSLLEPTVLPTRVPN
                     LLLNGASGIAVGMATNIPPHNLRELVNALVLLLDKPESTVKDLMRCVPGPDFPTAGFI
                     YGREGIREAYETGRGSIKMRARIEVEESGKRSHLIVTELPYQVNKARLLERIAELVKL
                     KKMEGIQDIRDESDREGMRIVLTLRQGEEPKIVENQLYKFTALESTFGVIMLAVVDNK
                     PEVLNLRDMLWHFLEFRRQMVVKRTQYELREAEKRAHVLEGLKKALDHLDEVIRLIRS
                     ASTPPEARRGLMERFEFSEIQAQAILEMRLQRLTGLEREKILEEYTNLLKEIERLRQI
                     LASPALVDQVVRDELKELTNQYGDLRRTEIVAEAGEICLEDLIADEEMVVTFSQAGYI
                     KRTPLAVYRSQRRGGKGRTGMSTREEDVVTAIFTASAHDILLVFTNRGQVFWLKVYEI
                     PEVGPAALGKAIVNLLPLQQGEKVQTVLPVKEFKEGHYVIMATRNGVIKKTELAAYAN
                     PRSTGIRAIVLDEDDELICARITDGNRHLFLMSRSGKCIRTEETEFRPLGRVSRGVRG
                     MNLDGSRLVGMDVIDEGKSILVVTEKGFGKRTPSEAYRPQSRGGKGVMNIRVTEKIGN
                     VIGFGQVGEDAEIIMITDRGRLIRVPVRQISKTGRVTQGVKLMDLGEGERLVDAAVLA
                     ESDEAKEDLDNE"
     gene            13642..14658
                     /locus_tag="PLM79_07900"
     CDS             13642..14658
                     /locus_tag="PLM79_07900"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOV86271.1"
                     /translation="MRFSGPFGVIGAGSWGTTLAQLLADKGYPVDLWVYEEDLCDIIR
                     STRENRYYLPGFLLHDNVSVHHDLGNVVKDHSLLVMVVPSHVYRRVAADMVPHLAPGT
                     VVVSAAKGIENETLLTMSGIWKEIAPRDGLVKVLSLSGPSFAKEVVQKMPTAVTLAGD
                     DIETAQDVQQVFSTGYFRVYTSLDKIGVELAGALKNVIALAAGAVDGLGFGHNTRAAL
                     ITRGLAEMCRLGVKLGAMPLTFSGLAGIGDLVLTCTGDLSRNRTVGFQLGQGRKLQEI
                     LGEMRMVAEGVKTAQSVHFLAKRMEVEMPISEQVYQVLYEDKNPADAARELMERDLKH
                     ELDL"
     gene            14836..16218
                     /locus_tag="PLM79_07905"
     CDS             14836..16218
                     /locus_tag="PLM79_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86272.1"
                     /translation="MIQELNPNIRERVREKKDSYHRYNFERLQEEAFATFFDLAQEYT
                     SLESLYEICVAVPKEFFSLESRLHVLEPQGSRLQMVCSSTTGLSTPEARVESLIPPAD
                     QSYETEEACVFPIRGNRALTQWIPFLQHGQVLGMFEIYPKQRVDAKSRFFLEKYTNRI
                     GYNLHQKLLIQQNLNHIKFINQLVADIEHNVISPNLYYKLFLLRLKKSVGEFEKIHGQ
                     FRDAVASVQETAPRLAGELEELCRALARNNQNLEEEREALTKHYEHTSLFLETLLRRD
                     HFEKGTYVLRKQSCNFRTEIIQPLLERYLPILEKKNIVVENGLEGVPDDQITLFVDKG
                     LISQVFDNLFSNAVKYAQQVEDHYGHRIKLMSCSRQILPDFFGSEIDGVRFNLFTTGR
                     SLTEKESKRIFDEGFRAKNSAHERGSGHGLHFVRHVVEIHGGVVGCEPQRYGNLFYFV
                     LPLKKQTLTP"
     gene            complement(16640..17923)
                     /gene="aroA"
                     /locus_tag="PLM79_07910"
     CDS             complement(16640..17923)
                     /gene="aroA"
                     /locus_tag="PLM79_07910"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOV86273.1"
                     /translation="MSVTVKKIQTVSKVKAFIRLPGSKSITHRALLMATLARGTSEIR
                     HPLVAEDTLLTARALEELGVGVSWEEDRVLVTPPSHRWSQPRNPILLGNSGTSMRLLP
                     AVAAVGSGRFVFDGTSRLRERPVAPVVDALTSLGARVRYLGAAGYPPIEIDASGLRGG
                     EAVVDARQSSQFLSALLIASPCSGGEVRIRWEEPVASFPYIALTLAMMEEMGLEYRWS
                     GATSITVPAPQVYPPFRFVVEGDCSSASYFWAAAALTGGEIHTVPVRRASAQGDCGFL
                     GVLEEMGCRVQWGDEGVTVKGTEDLKAVSRDMNTMPDMVPTLAVLAAFARGTSHIRNV
                     AHLRVKESDRLHVMAVELRKLGIPVEELPDGLKIEGGGARSPRESIEAHNDHRIAMAF
                     AVAGLRTPGIEIRGAESVAKSFPEFWEVFETLERN"
     gene            complement(18043..18882)
                     /gene="aroE"
                     /locus_tag="PLM79_07915"
     CDS             complement(18043..18882)
                     /gene="aroE"
                     /locus_tag="PLM79_07915"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOV86274.1"
                     /translation="MIQPVQKSLFAVIGNPVGHSLSPVMMNRAFQSLQVPAVYVALQV
                     DRLEEDLETLYRTGFEGLSVTLPHKEQALGIVEEADETARTIGAVNTLRRTPRGWEGR
                     NTDWLGANRALRAATPLEGKRVLVLGAGGAAKAVAFGLKKEGARVTIANRTVERGELL
                     ARSLGCRFLPLSQLSREGRESEFEIVVQCTSVGLPGSPPTSLVPRSFFRPSMVVMDTV
                     YRPLWTEFSSLARDAGSRIVPGWEMLLYQGVAQLEWWLGREIPEEGGIREMREALWEG
                     LVG"
     gene            complement(18879..19604)
                     /locus_tag="PLM79_07920"
     CDS             complement(18879..19604)
                     /locus_tag="PLM79_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOV86275.1"
                     /translation="MATRMTIPPRALCGCITDAPPGKLSAILENAAVDLLEWRLDAAI
                     HFHSLEAALQGIPLLSRSNRLPVVATHRPVSQGGHFSGPEKERMDLLQKAADAGAEWV
                     DLEEGTPEAMVRTFQRKGTQVLLSSHDFSGTPPGPSLRDLAKKMARKNPDAVKIVTLA
                     GNSEDCLRVLELIPFGQRELGLDVIAFAMGASGRWSRPASLLLGSPWAYVQLPGQPAA
                     APGQLEADEMRRLLAMLVGEAPS"
     gene            complement(19628..20758)
                     /gene="pheA"
                     /locus_tag="PLM79_07925"
     CDS             complement(19628..20758)
                     /gene="pheA"
                     /locus_tag="PLM79_07925"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOV86276.1"
                     /translation="MDEDLVRLREAIDEVDHELLRLLNQRMSMVRQIGRIKADKGISL
                     YDPGREESIFQRLIGANTGPLSNESLRSIYREIMAASRLLQAPLHVAFLGPEWTYSNL
                     AAVSMFGHSARYLSFPSLEEVFDALLKRKAQAAVVPVENSLQGGVGRTLDLLYEREVT
                     VVQECFLEVAHYLCGTGEDLRDLRQLYAHPQAMEQCRRWLLENLRNVETYECASTAQA
                     AQLARKNPSGAAICNLYAARHYGLNILAERIEDHAGNTTRFLALSDHANPPTGEDKTS
                     ILFAVPDQPGALHASLEAFARYRVNLSRIESRPNRLFSWQYTFFADLEGHREDENLQN
                     ALGDLKDRVTFLKVLGSYPKSDPKHPIRPEKEKMRSVRFGMS"
     gene            complement(20792..21832)
                     /locus_tag="PLM79_07930"
     CDS             complement(20792..21832)
                     /locus_tag="PLM79_07930"
                     /EC_number="1.4.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HOV86277.1"
                     /translation="MKEAWVFLDPWSKEMVTTALENGADALVLPPGFRESTQALGRIV
                     TVAPDGDMKPGRDVFFETLATHEDEMRIAGLSSSAPVVVELPGDPAGDSGIPTERHAG
                     TWRIIPLENLVARGGRILLSVHTREELDLAMGVMEKGVSGVVLHPQDPEELRELLVRV
                     KTREEPVALQTAVVETVRPAGMGDRVCVDTCTLMQEGEGLLVGNSSGFLFLVQAETIP
                     NPYAAPRPFRVNAGPVHAYVRVDRGKTRYLSELEAGDSVVIVRASGKAAPATVGRAKI
                     ERRPLVLLEASCGDEKGSILLQNAETIRLTSPDGRARSITELNPGDSVLAALEKRGRH
                     FGMEVDETIWEK"
     gene            complement(22115..25864)
                     /locus_tag="PLM79_07935"
     CDS             complement(22115..25864)
                     /locus_tag="PLM79_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86278.1"
                     /translation="MENRSPFGLMIPVAVLAALVLGLCGYAGPSSQGAAEEEAASATV
                     SQEADRLPTMTAFQGRSFDLIEEFRYGRKPQSSDPCRKHPADGHKVRFLIAMVPDPFD
                     SHIPYFDHYLAAVQRAVERAGFDLDRFYLPWPNSTQDEAARKQALSSRRFAEEAGIML
                     FRCDRCENKDLLLLFLVGETPTTGIHKKALRNALDQIAEFERRGMVFESDLRPLQSPG
                     KGDCLGGDGSGRFCGRQQTYMNIRILGPCFSGSAASLDFVLCEWFQQQRGRFDCEFNL
                     VTATATAVNRDRHFSPDHCDGLKIRFSSTSIPEDLALNAFLESQYCRDPRKVILLVEA
                     STAFGRGIPFQREWVAPHYRDRPLGGVRVYRFPFHVSQLRKEAATARTVPKATPSEMP
                     KKSQHFTTIPWEQAEQPRDTVPPLTDMEKPSVELMLRSLFTSIRRDGIEYVGIVATDV
                     MDLVFLARELRRYCPNTILFSFGSDLLYLHSDINVDLQGSLLLTPYPLVGLNQLWTYP
                     HKGDQFRHHFCTGFSMAVYNAAMILLEREEYLLEYGRPLVKPDRKVPRKPSLWLTAVG
                     REGMWPIDLLDYENHQEDVHYLHPSCSHKNDEADKRLIPLLPKDLVHPTMIGFFTFSF
                     FCMLVSWLVCREFPLRKSSRNGNRKKNGRSLGAWLRCFADVHLPEHRTGCRSTILGGC
                     ISLLAMYLIVTAIFILPVFLSIAHSSKGFHLCAITQWVVSLIALFALVSMLTAIGCLG
                     GVLWKERPTVRMQGGKGYRPDLLRSTRSMAFAWVSMGVLVLSLVLVGDWIFRALSRPD
                     RGLFLYLRSVFPESGLSPLLPLLLLTTASFLWSMRFVSRIRMNDKMACGGPFFGFNTE
                     PFGNMRKMESRLREVLSVSLIQSLRQFGRPAPWILIAITALPVFVLALRFVPTIEGRA
                     FDLVFGFSFSVVYGAVFLEFLRFVLLWVRLKSLLRLLKWLPLRVACAGFLDRFTGSNA
                     PPQQRLPKMDAATSVPPLNVLKVSVDQACHVFESIPALELPGELPPQLQATREHLLQR
                     LSDLRREAGEARWRYNLAIQAVTEDNQEELWDHGVRCQKALSNVSTILEEILGPLLRS
                     DLPDACGVKITGDKGSSKAGAWTQWTESASDFFILRIYHFLSCILPEFRGLVTFVLTG
                     ILLMLFAVGSYPFQPRQSILYFLWFVVLVVVAVTLAIYFRMNRDAVLSIFSGGTPHKI
                     DWGREFTLRVLGVGILPILAFLGVQFPEAVRQVLVWLGLTQAGQP"
     gene            26635..27069
                     /locus_tag="PLM79_07940"
     CDS             26635..27069
                     /locus_tag="PLM79_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV86279.1"
                     /translation="MLKSRIRIVRGDLTQQAFADQLDIHFRTVQRWEAGTRLPCADDL
                     VKIQQEFGININWLLTGTGPKYLRDLACPEPELKVCRIEDAPIERIKSWIQEIVEEQP
                     AYRGWLDIEFPKRFPEFKEWLEKKQRGESLENSSPMTHENSA"
BASE COUNT         5890 a         7873 c         8132 g         5432 t
ORIGIN      
        1 ccacccggga aagaggggtt gagctcaccc cgctaattca acatcctgct aaagggattt
       61 acctgggatt ttccccccgg ccttgcaatc gaccggaaaa tgaagtagat acggatcggc
      121 tcttttgact caagccggtc cgtgccaaga ggattcgctg attatcttcc ggattcgtcc
      181 acgccgtccc cttttttccc gagatgctgt tcccccgacc ccctctgcgg tacttccgac
      241 ggatgatgtt tccgccagct cccttcccca tgggatcgga gcctccgatt cggttccgtc
      301 cgcccggaca tgggttcatt tgggaacggc ccacgcggga gcctcggatc caacggcgat
      361 ggggaccccc tccgagccgt cccggacaat ccggttccaa tagggattcg ggggcctcac
      421 tccggaagga ggaggacccg aggttcaacc aggaatgtag gccggccaaa ccgaggaaaa
      481 cgaacgatga aatggttgaa gagtcttagt cttctcgact ttacggataa gggggattct
      541 cccgagcgct acgctctgct tcgccggaat atcctgattc tgatggtcat tgtcaccata
      601 tttcctcttt gtttcatggc cttagtcaac tatcaccaat accagaaggc cttgagggaa
      661 gagatcgtca atcccctgag aactttggtg aacaagacca agcattcctt cgagcttttc
      721 ctggccgagc gcctgtcgac tcttagcctt attgcctccg cgtacagctt cgatcagttg
      781 tccgacgaga acactctcac ccgtatcttt gccgccatgg agagggaatt tcgcggcttc
      841 gtggacctgg gcctcatcaa ctccgccggg gagcaggtca gctacgcggg gccctatgat
      901 ttgaggggca aagactattc ggaacagggt tggttccacg aagtccaagt gcgcggaagc
      961 tatgtcagcg acgtcttcat ggggtatcga ctatttcccc acgtggccat agccgtacaa
     1021 cggcggggcc ccgacggcag ggtctggatc cttcgggcca ccatcaacac cgagaagttc
     1081 gacgacctca tctcctccat gggcctcgag ccgtccagcg acgcgtttct tttaaacaag
     1141 agcggggtgt ttcaaacccc gtccaagcat ttcgggaacg tgttggatcc cttccctctg
     1201 cagttgcctc ccatcaccta cgaagccaac gtcattgaag agaagacccc tgccgggaag
     1261 ggggttctca tggcctacac ctatttcgtc aatgctccct acatccttgc ggtcgtcaag
     1321 ccgcagggcg tcatactcaa ggcctggtat acgctcaagt ccgagctctt cttcctgttt
     1381 ctcgggagtg ttgtggggac cttgctggtg atcttcaagc tcacggacgt cctggtccgc
     1441 agaatgaggg aatgcgacga aaaacgggaa gccgctttcc gggagatgca gcatacccac
     1501 aaattgtcct ccatagggag actggctgcg ggagtggccc acgaaatcaa caatcccatg
     1561 gccatcatca acgaaaaggc gggtctgatg aaggatctaa tggaattcat tccccaattc
     1621 cccgaacggg agaagtttgc ctctctgacg gatgccattc tccagtcggt gcaccgctgc
     1681 cgttcggtca ctcaccggtt gctcgggttt gccaagcgca tggaagtcca gatcgaagag
     1741 ctggacctca acgagaccat ccacgagacc ctgacctttc tcgagaagga agccctgtat
     1801 cgaaacctgg accttcgcct gaattggggg gccgatctgc ccagaatcgt gtccgaccgc
     1861 gggcagctcc aacaggttgc cttgaacctg ctcaacaact cctttgccgc cgtggaagac
     1921 ggaggcgtgg tcgaggtgac cacctggcga aaggatgccc gcaccgtggc tctcagcgtt
     1981 cgggacaacg gttgcggaat gtccccggag actctccgcc acatcttcga gccctttttc
     2041 accaccaaga aggactcggg aaccgggttg gggctcccca tcacctacgg catcatcaag
     2101 aaactgggcg gagacatcga agtgcaaagc cgggaagggg agggcacgac ggtctccgtc
     2161 ttcttgcccg tccaaatgca accctgaagc cggagaatcc cccatgcaga agtggaaggt
     2221 gctcctagtg gacgacgaga aggaattcgt ctcggcactt tcggaaagac tcatgttgag
     2281 aggcctggat gtcaggatcg ctttcagcgg ggaggaggcc ctcgggagcg tgcagtccga
     2341 tcctcctcaa gtggtcgtct tggacgtcat gatgcccggt ctgggagggg ctgaagtctt
     2401 ccgcaggatt cgagaacttc atccgaacat cccggtgatc tttctgacag ggcagggttg
     2461 ttccgtggat cggagacaaa ctgtgcccta cggggcgttt gagtgcctgg tgaagccgct
     2521 ggacatcgac gacttgattc gcaccatcag ctccgccatc ggcctgtgcg agaccccggc
     2581 actcctatcc gaatgaggac aggtaaagga aaagagggat atgcatcgat tctttccatt
     2641 cttgagctgg gtgaaaacca ttgattttac aacgtttcgc ctggatttcc tctccggaat
     2701 cacggtggcg ctcattctga ttccacaatc catggcctat gcccagttgg cgggaatgcc
     2761 cgtccactac ggactctatg cgtccctgct tcctcccatg ctggctgcgc ttttcgggtc
     2821 cagcaggcag ctggccacgg gtcccgtggc gatcgtgtcc ctcatgacgt ccgcagccct
     2881 ggcgcccctg gccacggcgg gcagcgattc ctatgttgcc tatgcgattc tgctggcgct
     2941 ggtggtgggg atttttcaat ttgccttggg ggtgttgaag ctggggctcg tggtgaattt
     3001 tctttcgcac cccgtggtga acggtttcac aaatgccggc gccctcatca tcgcctcctc
     3061 ccagttgtcc aagctcttcg atgtctacgt ggacgaggcc gaacaccact acgaaaccgt
     3121 gtaccgggtc gttcaggcgg ccctcgccta cacccaccgg cccaccctct ttttgggcgc
     3181 cctcgccttc ggcatcatgt acggcctcaa aagaatcgat cgcagaattc ccaacgtcct
     3241 catcgccgtg gtggtcacca ccctcatttc ctggttgacg ggctttgaac acaatcgaac
     3301 ggttcccctg gaggccgtcg ggtcggagga ggtccgtcaa ctcattgtgg gtttcaatga
     3361 agaagtccgg gccgtcgatc atcttgcggc caaaagggcc gccctgaccc cggaaatcga
     3421 aagcgccgcc aaacagtacg gagaaaattc cccccaggtg ctggaattgc gacacaaggt
     3481 tatgttgatg acggccaaga tccaggatct cagagcttcg tcgggggaca agaggtcccg
     3541 gcttcgcacc tgcctgttcc aggggattca agaaccggga aaagccctga tgctggttcc
     3601 ccgggaaacg gtttccgagg gaacgaaaac ggacggaaga acctggcgaa tcaaggtggg
     3661 caaccgccct ctcgatgaaa gcaaactcct gttcatgggc gggggctcgg tggtgggaga
     3721 gatccccaag gggcttccgg cactggaggt gccccgcatc gacctgggag tgatgatcca
     3781 tcttttgccc atcgccgcca tcatctccct gctggggttc atggaggcga tttccatcgc
     3841 caaggccatg gccgccaaga cgggacagcg cctggatccc aaccaggagc tcatcggaca
     3901 gggggtggcc aatatgttgg gtgccgtggg gaaaagtttc cccgtttccg gttccttttc
     3961 gcgctcggcg gtgaaccttc agtcgggtgc cgtgacgggg ctgtccagcg tggtcaccag
     4021 cactactgtt gggatcgtcc ttctcttttt cactcccctg ttgtaccatt tgccccagtc
     4081 ggtcctggcc gcagtgatca tgatggccgt ggtggggctg ctcaacgtct ccggtttcat
     4141 ccatgcctgg aaagctcagt ggtatgacgg cgtgttttcc gttctcacct tcgtggccac
     4201 gttgctcagt gctccccatt tggacaaggg aatcatgctg ggggtggtgc tctccctggg
     4261 aatgtttctc tacaagagca tgcgcccccg tgtatcgacc ctgtccatgt acgcggacca
     4321 gtcgtaccga agtgccagcc attacggctt gcgggaatgc aagcacattg ccctggtgcg
     4381 tttcgaaggg cagctctttt atgccaatgc gggtcacctc gaggagagca tctccaacct
     4441 gctcatggcc aagaaggagc tcaagcacat cgtgctggtg gccagcggaa tcaacgatat
     4501 ggatgccacc ggggaagaaa tgctctcctt ggtggtcgat cgcttgagga gcatggggta
     4561 tgatatgtcc ctgtgcggcg tgaacgaaaa cgtcctgggg gtgatgaagc gcacgcacct
     4621 gttggccaaa atcggcgaag aaaacgtgtt tcccaccatc cccgcggcgg tgaacgccat
     4681 tcatgccaga gctcacaccc agtgcagact cgatcattgc ccccttcaga gcattcgctt
     4741 tgtggaaaat gattagggat cggtcacccg aagtctgaga ggaaaaggaa caaaagccct
     4801 atgcccgtca ttgccatgat gttcggttct ttttgccacg gggaagaggt cgccgggaag
     4861 gttgcggccg aactggaata ttccctgttg aaggacgact ttttctttgc ccgggctgcc
     4921 gggaatttcc ggatggacga aggaaagctc cggaaggccg taacgggcaa gacctccgtc
     4981 ttcaaccagt tcacccacga acgggaacgc gccctggcct acctcaaggc gacccttgcc
     5041 gaatccttaa cgggggacaa cctcctcttg ctggggttcc ttgcccatct cattcccccc
     5101 gaaatttccc atgtcctgaa ggtgtgtctc atcgccgaca ttcgccgtcg agtccgggaa
     5161 gcccgccggg tcgaggggat ttccgaaaag gatgccttga agcggatcca caaggacgac
     5221 gaggggatcg tcctgtggac ggaatacctg ttccaacgaa aagacccgtg ggcgccggac
     5281 ctctacgaca tcctcctgcc catgggcaag gattcggtgg atcgagctgc ggcggtcatc
     5341 tgcgaaaacg tccggaagca ggtcctcatg gtttccgacc aatcccgccg gggggtcgag
     5401 gatttcatcc tggcctctcg agtgaacgtg aagctggccg aagagggcca tgatgtccag
     5461 gtgtcggcca gggacgggca cgttcgcgtc accatcaaca agaaggtgct gaggctatcg
     5521 gccctggagg aggagctgaa gaagctcgcc ctctcggtcc cgtcggtccg gagtgtcgag
     5581 acgcgggtgg gtgccgggta ttaccagacg gatatttacc ggaaatacga attcgatgtg
     5641 ccccttccca ccaaggtgct gctggtggat gacgagaggg aattcgtgga aacgctctcc
     5701 gagcgcctga ggatgcgcga ggtttcttcg gcggtggcct acaacgggcg ggaagccctg
     5761 gacgtcgtgg acgcggacga gcccgaagtg atggtcctgg acttgaaaat gccgggcatc
     5821 gacggactcg aagtccttcg gaaggtacga gacgaacatc ccgacgtgga ggtgatcgtc
     5881 cttaccggtc acggttccga ggaaatccga cggatttgca tggaaatggg ggcgtgcgcc
     5941 tacctggaaa agccggtgga aatggaaaac ctcaccgcgg cgatgaaaga agcctacgac
     6001 cgggtgaaac aaagaaaagc ccgtcaaaga tagccgtccc tgggccggac cgtgaaccgg
     6061 cgagcggcct tccccgactt ccgcggtccg ggaaggggcg ggtggaaaaa gaagaaggga
     6121 caacaacgcg tccgtgtcct gccggcgtgg gccggcagcc ggggacttat tgcggactga
     6181 ggctttcgcc gggggggcac gaaccgcgtt cggaggcggg cgaatcctcc tgcggtgctg
     6241 atccggggag ggcaaaagag ggtcccttac gggaggtgat ctcgtggaag actaccggaa
     6301 tcaagagctg gccttcttgg gcaaaatggc ggcgggattc acccacgaag tgaaaaacgt
     6361 cctggccatt atccgggaaa atgccgggct catcgaggat attcttacga tggcgcccgc
     6421 cgaggcagta cccaatctcg atcgagtcct gagagctctt tccgccataa gggaccagtc
     6481 cggccgggga gtggagctgt ccaaccggct caaccgtttt gcccactgcg cggatcagcc
     6541 cagagcgcat atcgagctca acgagaccct ggcccaattc accctcctgg tcgaaaggtt
     6601 tgcgcgactc aaagaggtca ccctcaccct ccatcgcggg gagagcggaa tcgaaatgga
     6661 aacccgcccc gttgggcttc aattggccct gttcggggtt ctggaatgtt gttggaacag
     6721 tgtctcttcc ctcagacggt tggatctaaa ggtggagagt gaaccgaacc agggggtggt
     6781 ggagattgtg ggcaaaggag aagatgatcg tcccgtgggt tttcacgaaa ccccggaaac
     6841 ggtggacgat tgggcccggg tggtctcccg gttggatgct ttgggcgcaa aggtggaacg
     6901 gggccctgcc gtggacgtgc tccgaattgt atttccgttg agatcgccct gaaccggatc
     6961 ggtgaggatg gagcgatgac tgacctggac gtgcttctgg tggacgacga gaaggaattc
     7021 gtggcggctc ttgcggaaag actcgccatg cgatgtctga aaacgaggat tgcgttcagt
     7081 ggggaagagg ccctggaact cgtgcggcaa aaggtccccg atgtggtggt tctggacctg
     7141 aaaatgccgg gcatgaacgg tctggacgtc ctcaggctcc tcaagaaatc tcatccccag
     7201 gtcccggtca tcatgcttac ggctcacggc tccgagcgag accgggaggc gggcaggctc
     7261 ctgggggcct tcgaccagct gcaaaagccc gtggagattg gggccctcat ccgatccatg
     7321 aaggaggcct gcggtcatcg gaggacgagg ccggaagcgg acaacggcgt gccgccccgc
     7381 ccggtgcagt gacaaggccg aaaaaccgtt cgggtcctgc aagagaatgc taccatcgcc
     7441 ccgcttcccc aagagaatct cccggatcgc ggctgcggct acggcaagaa gccgggggat
     7501 ttcaggggtc ggatcgatga cctttgaggc cggaagaggg gctgaaacaa gccgttccgc
     7561 acacaatgcc gggaacgtcg acgtcggagt agaggtcatt gggcataaga ctgttgttgg
     7621 tggacgatga agaggatttt tcccggacgg tggccgagag gttgaccctg agggggatga
     7681 atgtgcgcat tgcttccagc ggggaggacg ccctccagcg aatggaggaa tcgcctccgg
     7741 acgtggtggt cctggatgtg gtgatgccgg ggatgggggg gctggaaatc cttcgctgga
     7801 tcaaaaaccg gcacccttcc atccaggtga ttcttctttc ggggtttgct tcttcccagg
     7861 acgcccggaa gggcatgacc atgggcgctt tcgattacct gctcaagccc gtggaccttg
     7921 aagaattggt ccgcaagatc ctggacgggt atcgggccat ggagcggtga tgtgcgtcaa
     7981 aatcgagcga tcgcctttag cgtttagcca ttcgcttcga catttcctgt ggtggagctg
     8041 ccttggcttc catccaaccg ggtgacgtcc gaaaattcca aggcacaatg ccccgaagag
     8101 ctaatacgaa gttgcattca agccgaaact gcccacccgt aggattcatc cctttgctcg
     8161 aaatcttcac tcagcatgtc agggcgacct tgggcgcaac ctggtaaaac ggcaaatcgc
     8221 tccatgccga aattcggcga atcctgctcc atcgaggccg gggatagtcc ccggggggtt
     8281 gaggcggcac gggggccttc ttccccggtc gttccgcatt catgacccgc tccgaggtcg
     8341 tattggaggc agtctccacg gccggggttg ggaaaacctc tccgcccgtt ttcgcaaatc
     8401 cagggggagc tccgcccctc tttccgtcag gtttttccgc agatcgaggc accccccttg
     8461 tgtttccgcc cgggagacgc atgggtctga aaattcctct tcccattgaa aaatttctaa
     8521 aaaaaaggta tagttacctg ttttcaaaac ccaccgatcc gggcctggtt ccccgtggaa
     8581 aatactcgga aaacaggccg gttccggggg ctccttcttc tttcgactcc gggagaaagg
     8641 aaggaattcg ggacttcgag atctttttcg gcaccggcgg gggatcgagg gattcctgcc
     8701 ccgcgggtcc acacggcgga aacgccgtcg ctctcccaag aaacgaggtt tttatgccca
     8761 gtttggaagg caccgacaag gcggcaacgg atcaatatac ggcgcagcag atcacggtcc
     8821 tggagggact cagcgccgtg cgcatgcgcc cttccatgta tatcgggaac gtggccgtgg
     8881 aggggcttca tcatttggtc tacgaggtgg tcgacaacag catcgacgaa gccctggcgg
     8941 gattttgcga ccacatccgg gtgcagattc acctggacgg ctccgtcacc gtggacgaca
     9001 acgggcgggg aatccccgta gacacccatg agaaagagaa tcttcccgcc gtggaggtgg
     9061 tcatgaccaa gctccacgcg ggaggaaaat tcgacgattc cacctacaag gtatcgggag
     9121 ggctccacgg ggtgggagtc tccgtggtga acgctctgag cgagtttttg gacgtggaga
     9181 tccgccggga ggggaaggtg tattcccagt cttacgccag gggagacgcg gtaactcccc
     9241 ttcgggtgac gggggagacc tcccgcaggg gaactcgaat caccttcaaa cccgatcccc
     9301 tcattttttc cgacaccgag ctcagcttcg acatattggc ccagcgtttc cgggagctgg
     9361 cgtttctcaa ccccggcgtg agcattcatc ttctcgacga gcggattccc cgggagagag
     9421 agttttgtta ccagggcggg ctggtgtctt tcgtcgagta tctgaacaag aacaaggaac
     9481 ctctccaccc ggaaatcatt ttcatttccg gggaccggca ggggatttcc gtcgaaatcg
     9541 ccatccagta caaccagacc tacaacgaaa agatttttac ctttgccaac aatatccaca
     9601 ccaaggaagg cgggacccat ctcatcggct tcaaggcggg gcttactcgg gccatcaagc
     9661 agtacgccgt tcaaaccaag atggccaaga gcgacctgga caaactgacg ggagacgatg
     9721 tgcgtgaagg tctcacggcc gtcttgagcg tcaagctgtc ccagccccag ttcgaggggc
     9781 agaccaagac gaagttggga aacagcgagg tcaaagggct tgtcgagaac ctgacctatg
     9841 agaagctctc cgcatttttc gaagagaacc ccaaggtcat tcgcagcatt ctggacaagg
     9901 tgctcgaggc ggcccgggcc cgggaagcgg cccgcagagc caaagaactc acgcggcgca
     9961 agggagtgct cagcgaccat tctcttcccg gcaagttggc cgactgccag gaacgggacc
    10021 cgtccaagag cgaaatcttc atcgtcgaag gagattcggc cggaggctcg gccaaacagg
    10081 gtcgagatcg gaggttccaa gccatccttc ccctgcgggg aaaaatcctc aacgtcgaga
    10141 aggcccgctt cgacaaaatg ctcgagaacc aggaaatccg caccatgatc acggcccttg
    10201 gaacggggat cggaaaggac gaatacgatc ccgacaagct gcgctatcat aagacgatca
    10261 tcatgaccga tgcggatgtg gacggggctc acattcgaac cctgcttctt accttctttt
    10321 tccgccagat gccggacatg atcgagcggg gccatctttt catcgcccag ccgcccctct
    10381 atcgggtggc ggcggggaaa cacgagcaat acctcaagga cgaggacgaa ttgaacgcat
    10441 acctgctgaa acgggccgtg gaaaagaaat cggtgacctt gcccgccctg gattccccca
    10501 ttcctcccga gcaactgatt cagctcctca agacctactc ccggtacgaa gaatggctcg
    10561 agcgccagga acagaagggg atgaccaagg cgctgctcga tttggccatc cggattttca
    10621 gccgcaacaa gctggtgccg tcccacgagg agagcatgtc ggtgcttcgc atggaactcg
    10681 agaaggcggg ctacgaggtg gaatccatgg agctcgagga ggaacaccgg ggctacgatc
    10741 tcgaggtctg gcagcccacc aacggacagc agcgcattcg gcttacctac gacttccttc
    10801 aatccatcga attcagaaaa ctcctggaac tctacgatca gctcgaggtg ctccaccaaa
    10861 ccccctacac ggtaaaggac gcgaacaagg aagtccggta cgacgaccct cgtgccctgt
    10921 ttcactccct catggacgaa gcccgcaagg gtttgaccat ccagcgctac aagggtctgg
    10981 gggagatgaa tcccgagcag ctctgggaca ccaccatgaa cccggaaaag cgaaccctcc
    11041 tccaggtgag gatcgaggat cagtaccagg ccgacgagct gttcaccacc ctcatgggcg
    11101 atcgggtgga accccgcagg gatttcatcc aggtgaacgc cctggacttc cgggaactcg
    11161 acatttagcc atttccattc atcggcttct cacccactag gaacgagatt ccatggacgc
    11221 tcgcaccatc aatatcgagg acgaaatcaa gctatcctat ctggattatg ccatgagtgt
    11281 gatcatcggg cgggccatcc cggacgtgag ggacggcctc aagcccgtgc accgtcgcac
    11341 cctgttttcc atgcatgaac tggggaacga ttacaacaag gcatacaaga aatccgccag
    11401 gatcgtgggg gacgtcatcg gcaaatacca tccccacggg gaatccgccg tgtacgatgc
    11461 cctggtccga atggcccagg atttttccat gcgctacacc cttgtggacg ggcagggcaa
    11521 cttcggctcc atcgacggag atcctcccgc ggccatgcgg tacacggaag tgcgcatgag
    11581 tcgcctggcc ggggagttcc tgcgggacat cgaaaaggaa acggttccct ttcagcccaa
    11641 ctacgacaat tctttgctcg agcccacggt gttgcccacc cgggtgccca acctgcttct
    11701 gaacggggct tcgggaatcg ccgtggggat ggcaaccaac atccctcccc acaacttgcg
    11761 ggagctcgtc aacgccctgg tccttctcct ggacaaaccg gaatccacgg tcaaggacct
    11821 gatgcgctgc gtgcccggcc cggattttcc cacggccggt ttcatttacg ggcgcgaggg
    11881 aatccgtgag gcctacgaga ccgggcgggg ctccatcaag atgcgggccc gaatcgaagt
    11941 ggaagaatcg ggcaagagaa gccacctcat cgtcacggaa cttccctacc aggtgaacaa
    12001 ggcgcgcctg ctcgagcgaa ttgccgagct ggtgaagctc aagaagatgg agggcattca
    12061 agacatccgg gatgaatccg accgggaggg catgcgcatt gtgctcaccc tccgccaggg
    12121 ggaggagccc aagatcgtcg agaatcagct ctacaagttc acggccctcg agagcacttt
    12181 cggggtgatc atgctggccg tggtggacaa caagcccgaa gtcctgaacc ttcgggacat
    12241 gctgtggcat ttcctggaat tcaggaggca gatggtcgtc aaacggaccc agtacgagct
    12301 gcgggaggcc gagaagcggg cgcatgtctt ggaggggctc aagaaagccc tggaccacct
    12361 ggacgaggtg atccggctga ttcgatcggc ttccactccc cccgaggctc ggcgcggcct
    12421 gatggagcgg ttcgagttct ccgaaatcca ggcccaggca atcctcgaga tgcggctgca
    12481 gcggttgacc ggcctcgagc gggaaaagat cctcgaggaa tacacgaacc ttctcaagga
    12541 aatcgagcgg cttcgccaga tcctcgcttc tccggccctg gtggaccagg tggttcggga
    12601 tgagctcaag gagctgacga accagtacgg agacctgcgc aggaccgaaa tcgtcgcgga
    12661 agcgggcgaa atctgcctgg aagacctcat tgccgacgaa gaaatggtgg tcaccttctc
    12721 ccaggccgga tacatcaaga gaactcccct ggcggtctac cgaagccaga gacgaggggg
    12781 gaaagggcgc acgggcatga gcacccggga agaagacgtg gtgaccgcca ttttcacggc
    12841 ttccgcccac gacatcctcc tggttttcac caatcgcggc caggtcttct ggctcaaggt
    12901 gtacgagatt cccgaagtgg gacctgcggc cctggggaag gccattgtga accttcttcc
    12961 cctgcagcag ggggaaaagg ttcagacggt cctgccggtc aaggaattca aggaagggca
    13021 ctacgtgatc atggccaccc ggaacggggt gatcaagaaa acggagctcg cggcctatgc
    13081 caacccgcgg tccacgggaa tccgggccat cgtgctcgat gaagacgacg agctcatttg
    13141 cgctcgaatc accgacggga accgccacct gttcctcatg tcccggtcgg gcaaatgcat
    13201 tcggacggag gaaacggagt ttcgccccct ggggagagtg agccgcgggg ttcgcggaat
    13261 gaacctggac ggcagccggt tggtgggcat ggacgtgatc gacgagggaa agagcatcct
    13321 cgtggtcacg gagaagggat ttggaaagcg gactcccagc gaggcgtatc gcccccaaag
    13381 tcgagggggc aagggggtca tgaacattcg cgtcacggaa aagatcggaa atgtgatagg
    13441 gttcggacag gtgggcgaag atgcggaaat catcatgatc acggatcggg gccgcctgat
    13501 tcgagtgccc gtgcggcaga tcagcaagac cgggcgggtg acccagggag tgaagctcat
    13561 ggatttgggc gagggggagc gactcgtgga tgcggcagtg ctggccgaat cggacgaagc
    13621 caaggaggat ctggataacg aatgagattt tcgggtcctt tcggagtcat aggcgcggga
    13681 agctggggaa cgaccctggc tcaactgctg gcggacaaag ggtatcccgt cgatctgtgg
    13741 gtgtacgagg aagacttatg cgacatcatc cgcagcactc gggaaaaccg ctattatctg
    13801 cccgggtttt tgttgcatga caacgtttcc gtccatcatg acctgggcaa tgtggtgaaa
    13861 gatcattccc tgctggtcat ggtggtccct tcccatgtgt atcggagggt ggccgcagac
    13921 atggtccctc acctcgcccc ggggacggtg gtggtgagtg ctgccaaggg aatcgagaac
    13981 gaaaccctgc tcaccatgtc cggaatctgg aaggagattg cgccccggga cggactggtg
    14041 aaggtgctga gcctttcggg accctctttt gccaaggagg tcgtgcagaa gatgcccacg
    14101 gccgtcactc tggccgggga tgatatcgag acggcccagg atgtccagca agtgttctcc
    14161 accggctatt ttcgggtgta caccagcctg gataaaatcg gcgtcgagct ggcgggggcc
    14221 ctcaagaacg tcatcgccct tgcggcggga gccgtggacg ggctgggttt cgggcacaat
    14281 acccgggccg ccctcatcac ccgggggctg gcggaaatgt gtcggctggg ggtgaaactg
    14341 ggggccatgc ccctgacctt ctccggcctg gcgggaatcg gggacctcgt cctcacctgc
    14401 accggggatc tgagccgcaa tcgaacggtg ggctttcaac tggggcaagg cagaaagctc
    14461 caagagatcc tgggggagat gaggatggtg gccgaaggag tcaagaccgc ccaatcggtt
    14521 cacttcctgg caaaacgcat ggaagtggaa atgcctattt ccgagcaggt ctaccaggta
    14581 ctctatgaag acaaaaaccc ggcggatgcc gcccgggaac tcatggagcg ggacctgaag
    14641 cacgaactgg atctataaga atcagacttt tttgcaggat tctctccgtt ttttgacttt
    14701 tcctgacccc ggggggccgt agttgcggta ccatggaagc cgccgcgtcg gctgaacaac
    14761 cacggccgct tcgatgggca aaagtgaggg ctgctggagt tagacctctt tacatctcag
    14821 ccgaggaacg atgtcatgat ccaggagctc aatcccaaca tccgggaaag ggtgagggaa
    14881 aagaaggaca gctaccacag gtacaatttc gagcgcctgc aggaagaagc cttcgccaca
    14941 ttttttgacc tggcccaaga atacaccagc ctcgaaagtc tctacgaaat ttgcgttgcg
    15001 gtgcccaagg agttcttttc cctggaaagc cgccttcatg tgcttgagcc ccagggttcg
    15061 aggctgcaaa tggtctgttc ctcgaccacg ggactgagta cccccgaggc ccgggtggaa
    15121 tccctcattc ccccggccga ccaatcctat gaaacggaag aagcctgtgt ctttcccatc
    15181 cgtggaaatc gtgccctcac ccaatggatt ccgttcctac agcatggaca ggtcctgggg
    15241 atgttcgaga tctaccccaa gcagagggtg gatgccaaga gccgcttctt tctcgagaag
    15301 tacaccaatc gaatcgggta caatcttcac cagaagttgc tcattcagca gaatctcaac
    15361 cacatcaaat tcatcaatca gcttgtggcc gacatcgagc acaacgtcat ctctccgaat
    15421 ctgtattaca agctgtttct tcttcgtctg aagaagagcg tgggagaatt tgagaagatc
    15481 cacgggcagt tccgggacgc cgtggcctcc gtccaagaga cggcgccgcg attggcaggg
    15541 gaactcgagg agttgtgccg ggccctggcc cggaacaacc aaaacctgga agaggaacgg
    15601 gaggccctga ccaagcacta cgaacacacc agtttgtttc tggaaacgct gctgcgccgg
    15661 gaccatttcg aaaaaggaac ctatgtcctg cgcaaacaat cgtgcaattt ccgcacggag
    15721 atcattcagc ccctcctgga gcggtatctt cctattctcg aaaagaagaa catcgtggtg
    15781 gaaaacggtc tcgagggggt tcccgacgac cagatcacgc tgtttgtgga caaagggctc
    15841 atttcccagg tcttcgacaa cctgttttcc aatgccgtga agtacgccca gcaagtggaa
    15901 gaccactacg ggcatcggat caagttgatg tcctgcagtc gacagatcct gcccgacttt
    15961 ttcggctcgg agatcgacgg agttcgtttc aatctcttca ctacgggaag gtctcttacc
    16021 gaaaaagaat ccaagagaat tttcgacgaa ggatttcggg cgaaaaactc ggcccatgaa
    16081 agggggtccg gccatgggct tcatttcgtc cgtcacgtgg tggagattca cgggggagtg
    16141 gtggggtgcg aaccccaaag gtacggcaac cttttctact ttgttttacc cctcaagaaa
    16201 cagaccctga ctccttgacg ggggaccgcc gggcgctttt tccccctctg ttgatgcccc
    16261 tcatggaact acaggatcgg gaatccctca aatacagcct tcaccccatt aacaaccggg
    16321 atggactgaa ataatttgtg agtcaggagg tcgattcctg acttctactc tccgagtagg
    16381 gggtactccc ctcaatcccc tgctcttacc cgcctcactg cactgccgtt ttcagggagg
    16441 gtggccacaa aaggaatccg ggtatttccc ctccctgaaa acgagcagtc ccccgaaacg
    16501 tatctgcagc cttcgggatg ccgcggttga aaccccaccc ttgccggctc gttcggttcc
    16561 gggattcgtg tttcccaggg ggcaggagtt gttcgaggtc gtggctcgac ggcggaaggg
    16621 gtccggggct tcccggacct cagttgcgtt ccagggtttc gaagacttcc cagaattccg
    16681 gaaaagattt ggccacggat tcggctccgc ggatctcgat tcccggcgtc cgaagcccgg
    16741 ccacggcaaa ggccatggca attctgtgat cgttgtgggc ctcgatggac tcccggggag
    16801 aacgagcccc tcccccctcg atctttagac cgtcggggag ttcctcgacg ggaattccca
    16861 acttgcgcag ttcgacggcc atcacgtgca gccggtccga ttccttgact ctcaggtgcg
    16921 ccacgttgcg gatgtgggag gttccccggg cgaaagctgc caggacggcc agggtgggaa
    16981 ccatgtccgg catggtgttc atgtcccggg aaacggcctt caggtcctct gtgcccttga
    17041 cggtgacccc ctcgtccccc cattggaccc ggcatcccat ttcctcgaga acgcccagga
    17101 atccgcagtc cccctgggcg cttgcccggc gcacgggaac ggtatggatc tccccccctg
    17161 tgagtgccgc cgccgcccaa aaataggaag ccgacgaaca atccccctcg accacgaacc
    17221 ggaagggcgg gtaaacctgg ggggcgggaa cggtgatgga tgtggcaccg gaccaccggt
    17281 actccagtcc catctcctcc atcatggcca gggtaagggc gatgtaagga aaggacgcca
    17341 cgggctcttc ccaccgtatc cgcacctccc cccccgaaca gggggatgcg atgaggaggg
    17401 cggacaagaa ctgactgctt tgacgggcat ccaccacggc ttctcccccc cggagccccg
    17461 aggcgtcaat ctcgatggga ggataccccg ctgcgcccag gtagcggacc cgagctccca
    17521 gggaggtcaa agcgtccacg acgggggcga cgggccgctc ccgcagtctc gatgtcccgt
    17581 cgaacacgaa tcggccggac ccgacagcgg cgacggcggg cagcagacgc atgctggtcc
    17641 cgctgtttcc caaaagaatc ggattgcggg gctgagacca tcgatgggaa gggggggtca
    17701 ccagtacccg gtcctcttcc caggaaaccc ccactcccag ctcttccagg gcgcgggcgg
    17761 tgagcagggt gtcttcggca accaggggat gccggatttc gctggtccca cgggccaggg
    17821 tcgccatgag cagagcccgg tgggtgatgg acttggatcc cggcaaacgg atgaaggcct
    17881 tcaccttcga aacggtttgg atcttcttta ctgtgacgct catggtttca tcctgattcc
    17941 ccgcgacctt tccaaagccg gtggtgagct aagaaacgcc gccgttccgc atcctgcctc
    18001 atggtttctc ttccacaccc tgcaacccaa ccaatcaacc aactaaccaa ccaacccctc
    18061 ccaaagcgcc tcccgcattt cccggatccc gccttcttcg gggatttccc ggcccagcca
    18121 ccactccagt tgagccactc cctggtagag gagcatttcc cacccgggaa cgatccggga
    18181 acccgcatcc cgggccaggg aggaaaactc cgtccacaag ggtcggtaga ccgtatccat
    18241 gactaccatg gagggtcgaa aaaaggacct gggaaccagg gaagtcggcg gagagcccgg
    18301 gagcccgacg gaagtacatt ggaccacgat ttcgaactcg ctctctcttc cctctcgaga
    18361 cagctgggac aggggaagaa aacggcatcc cagggaacgg gccaggagct ccccccgctc
    18421 gacggttcga ttggcaatgg ttaccctcgc tccttccttt ttcagcccga aggccacggc
    18481 cttggccgcg cccccggctc ccaggaccag cactcttttt ccctcgaggg gagttgctgc
    18541 gcggagggcg cggttggccc ccagccagtc ggtgtttctc ccttcccatc cccggggggt
    18601 cctgcgcaag gtgttgacgg cgccgatggt ccgggccgtt tcatccgctt cttccacgat
    18661 ccccagggct tgctccttgt gaggcagagt gacgctgagc ccctcgaacc ccgttcgata
    18721 caaggtttcc aggtcttctt ccagtcgatc cacctggagg gccacgtaca cggccgggac
    18781 ctgaagactt tggaacgccc ggttcatcat gacggggctc aggctgtggc ccacggggtt
    18841 tccaatgacg gcaaacaggc ttttctgaac gggctgaatc atgaaggggc ctctccaacc
    18901 aacattgcca ggagccttcg catctcgtcg gcttccaatt gtcccggggc cgcggcgggt
    18961 tgccccggga gctgcacgta ggcccacggg ctccccagga gcaacgatgc cgggcggctc
    19021 cagcggcccg atgcccccat ggcgaaggcg atgacgtcca ggcccagctc ccgctgtccg
    19081 aaggggatga gctccaggac tcggaggcag tcttcggaat ttccggccag ggtgacgatt
    19141 ttcacggcgt cggggttctt gcgggccatc tttttagcca ggtctctcag gctgggcccg
    19201 gggggggtcc ccgaaaaatc gtgggaggaa aggagcacct gagttccctt gcgctgaaac
    19261 gtccgcacca tggcttcggg ggtcccctct tcgaggtcca cccactcggc cccggcgtcg
    19321 gcggcctttt gcagcaagtc catgcgctcc ttttccggtc ccgaaaaatg acctccctgg
    19381 gacacgggac gatgggtggc caccaccgga agccggttgg aacgggacag gaggggaatg
    19441 ccctggaggg ctgcctcgag ggagtgaaaa tgaatggcgg cgtccagtcg ccattccagg
    19501 agatctacgg cggcattctc caggatggcg gagagctttc cgggaggggc gtcggtgatg
    19561 cacccgcaca gggctcgagg cggaattgtc atccgtgttg ccatgaattt ctcccatgcc
    19621 cgagccgtca tgacatgccg aaccggaccg atctcatctt ttccttttcg gggcggatgg
    19681 gatgcttcgg gtcgctcttg ggataggacc ccagcacctt caggaaagtc acccggtcct
    19741 tgaggtcgcc gagggcgttt tgcaggtttt cgtcttcccg gtggccttcg agatcggcaa
    19801 agaaggtgta ctgccaggaa aagagacggt tggggcgcga ttcgatgcgg ctgaggttca
    19861 cccggtatcg ggcgaaggcc tcgagggagg cgtgaagggc cccgggctgg tcgggaacgg
    19921 caaagaggat cgaggtcttg tcctcgcccg tgggagggtt ggcgtggtcc gagagggcca
    19981 ggaaccgggt ggtgtttccg gcgtggtcct cgatgcgctc ggccaggatg ttcaatccat
    20041 aatgccgggc tgcgtacaaa ttgcatatgg ccgccccaga ggggttcttt cgggcaagct
    20101 gcgcggcctg ggcggtggat gcgcattcgt aggtttccac gtttcgaagg ttttcgagga
    20161 gccaccgcct gcactgttcc atggcttgag gatgggcgta gagctgccgg aggtcccgga
    20221 ggtcttcgcc ggtcccgcac aggtagtggg cgacttccag gaagcattcc tggaccaccg
    20281 tgacctcccg ctcgtacaac agatccaggg tgcgccccac gcctccctgc agggagtttt
    20341 ccacggggac cacggcggcc tgggcttttc tcttcaagag agcgtcgaat acctcttcca
    20401 gggaaggaaa ggagaggtag cgggcggagt ggccgaacat ggacaccgcg gccaggttgg
    20461 aataggtcca ttcgggtccc agaaaggcca cgtgaagggg ggcctggagc agccgggatg
    20521 cagccatgat ctcccggtag atggagcgca gggattcgtt ggacaacggt ccggtgtttg
    20581 ccccgatcag gcgctggaag atgctctcct cccggcccgg atcataaagg ctgatcccct
    20641 tgtccgcttt gattcgccca atctgccgca ccatggacat tctttggttg aggaggcgga
    20701 gtagttcatg atccacctcg tcaatggcct cccgaagtct tacaagatcc tcgtccacga
    20761 aaatctccct atgggtcttg gttgttcgag cctacttctc ccagatggtc tcatccactt
    20821 ccatgccgaa atgtcttcct cgcttttcca aggccgccag caccgagtcc cccgggttca
    20881 attccgtgat ggaacgggct cgaccgtccg ggctggtcag tcggatggtt tcggcgtttt
    20941 ggagcaagat ggagcccttc tcgtctccgc aggatgcctc gaggaggacg agaggcctcc
    21001 tttcgatctt cgccctgcct accgtggccg gagcggcttt tcccgaggcc cggacgatga
    21061 ccacggaatc cccggcttcg agttcgctga ggtaccgggt cttccctcga tcgacccgca
    21121 cgtaggcatg aaccggcccg gcgttcaccc gaaacgggcg gggtgccgcg tagggattgg
    21181 ggatcgtttc cgcctggact aggaagagaa acccgctgga atttcccacc agcagcccct
    21241 ctccttcctg catgagggtg caggtgtcca cgcacacccg gtcccccatt cccgcggggc
    21301 gaacggtttc aaccacggcg gtctgaagcg ccacgggctc ttcccgggtc ttgacccgca
    21361 cgaggagctc tcggagctcc tcggggtcct gcgggtgaag gaccaccccg gagactccct
    21421 tttccatgac ccccatggcc aggtccaatt cttcccgcgt atgaacggat aggaggatgc
    21481 gtccgcctcg agccacgagg ttttccaggg gaatgatccg ccaggtcccg gcgtggcgct
    21541 ccgtcgggat gccggagtcc ccagcgggat ctccgggcaa ttccaccacc acgggagccg
    21601 aggacgacag cccggcgatc ctcatttcgt cctcgtgggt ggcgagggtt tcgaagaaga
    21661 cgtcccgtcc cggcttcatg tctccgtcgg gggcaaccgt gacgatccgg cctagggcct
    21721 gggtgctctc ccggaacccc gggggaagaa cgagggcgtc cgcaccgttt tccaaggccg
    21781 ttgtcaccat ttccttggac cagggatcga gaaaaaccca ggcttccttc acgaccgaac
    21841 ctcctccatt ccgaccgaac ctcctcgatt cccccttctt cgtctcaaaa acaaaaagcc
    21901 gcggggctga acccgcggct ttgaagtctt tggcaagaga ggaaccctgg caggtcagcg
    21961 tggcgtcgct accctcgtat cataaaaata aaaaaaagaa tcacatgcgc gatgcatagg
    22021 ccgaaccgag gctcctttcc tcattgagtc gtcaacataa aggcaccatc gggaaactgt
    22081 caagggaaaa aatccacaat ccggggccgg ggcatcaggg ttgcccggct tgggtcaagc
    22141 ccagccagac aaggacctgc ctcactgctt ccgggaactg gacccccagg aatgccagga
    22201 tgggcagaat cccaactccc aggaccctca gggtgaattc ccttccccag tcaatcttat
    22261 ggggcgtgcc ccccgaaaag atgctcaaaa cggcatcgcg gttcatccgg aaatagatgg
    22321 ccagcgtgac cgccaccacg acgagaacca caaaccaaag aaagtagagg atggactggc
    22381 gtggttgaaa agggtagctg ccgacggcaa agagcatcag caggattcct gtcagtacga
    22441 aggtgaccag accgcgaaat tccggtagaa tgcaggacaa gaagtgatag atcctcagaa
    22501 tgaagaaatc cgatgcggat tccgtccatt gtgtccaggc tcccgctttt gaagatccct
    22561 tgtccccggt gatcttgacc ccgcaggcat cggggaggtc ggatcgaagg agcgggccga
    22621 ggatctcctc aaggattgtg gagacgtttg aaagggcttt ctgacaccgg accccatgat
    22681 cccatagctc ttcctggttg tcttcggtta cagcctggat ggccaggttg taccgccacc
    22741 gagcttctcc cgcttcccgt ctcaggtccg aaaggcgttg cagcaaatgt tcacgggtgg
    22801 cctgcaactg cgggggcaat tccccgggaa gttcaagggc ggggatggac tcgaagacat
    22861 gacaggcttg atctaccgag actttcaaca cgttaagggg cgggacggag gtggccgcat
    22921 ccattttggg gagcctctgt tgtgggggtg cgttgctgcc ggtgaagcga tcaaggaacc
    22981 ctgcacaggc gactcggagc ggtagccatt tgaggagtcg aagaagactt ttcaatcgca
    23041 cccacagaag aacaaagcgg agaaactcta agaaaacggc cccatagaca accgagaaac
    23101 tgaatccgaa aaccaggtcg aaagcccttc cttcgatcgt gggaacaaac ctcaaggcca
    23161 gaacgaagac gggcaacgcc gtgatggcga tgaggatcca cggtgccggc cggccaaact
    23221 gacggagtga ttggatgagg gatacggaaa gtacttccct caaacgagat tccattttcc
    23281 tcatgtttcc gaagggttct gtgttgaacc caaagaaggg ccccccacag gccatcttgt
    23341 cgttcatgcg aatgcggctg acgaatcgca tcgaccacag gaaactcgcc gtggtcagca
    23401 ggagcagagg aaggagaggc gagagcccgc tttccggaaa gacgcttcgc agatagagga
    23461 aaagccccct atccgggcga gagagagcgc gaaagatcca atcccccacc agcacaaggc
    23521 ttagcaccag gacccccatg gaaacccagg cgaacgccat cgaacgagtg ctccggagga
    23581 gatcgggcct gtatcctttt cccccctgca ttcgaaccgt tgggcgttcc ttccaaagga
    23641 caccccccaa acagccaatt gccgtgagca tggaaaccag ggcgaacagg gcaatgaggg
    23701 aaacgaccca ttgcgtgatt gcgcaaagat ggaagccctt cgaggagtgg gcaatactca
    23761 ggaagacggg cagaatgaag atcgcggtca cgatcaggta catggcaaga aggctgatgc
    23821 atccgccaag gatcgtcgag cgacaaccgg ttcgatgttc aggaagatgg acgtcggcaa
    23881 agcaacgcaa ccaagcaccc agcgatctcc cattcttctt tcggttgccg ttcctggaag
    23941 atttccgcag gggaaactca cgacacacca gccatgaaac cagcatacag aaaaagctga
    24001 aggtaaagaa cccgatcatg gtcgggtgaa caaggtcttt gggaaggagg gggatgagcc
    24061 tcttgtcggc ttcatcgttc ttgtgactgc aggatgggtg caaataatga acgtcctcct
    24121 ggtgattctc gtagtccagg agatcgatgg gccacatccc ttcccgcccg acggcggtga
    24181 gccacaagga cggtttgcgc ggtacctttc ggtcgggctt tacaagcggc ctaccgtatt
    24241 ccagcaggta ttcctccctt tccaggagga tcatggctgc gttgtaaacc gccatggaaa
    24301 accccgtgca aaagtgatga cgaaactgat ctcctttgtg cgggtaggtc cagagctggt
    24361 tcagaccgac aagggggtag ggcgtcagca acaggcttcc ctgcaaatcg acgttgatat
    24421 ccgaatgcag atacagaaga tcggacccga aggagaaaag gatcgtattg gggcaatacc
    24481 gtcggagttc tcgggccaga aagaccaggt ccatgacgtc ggttgccaca atgcccacat
    24541 attcaatccc gtcgcgcctg atcgaggtga aaaggcttct caacatgagc tcaacggacg
    24601 gcttctccat gtcggtcaag gggggaaccg tgtctctggg ctgttccgct tgttcccatg
    24661 gaatggtggt gaaatgctgg gacttcttcg gcatttcgga aggagttgcc ttggggacgg
    24721 tcctggccgt tgccgcctct ttgcgcaact gggaaacatg gaaggggaaa cgatacaccc
    24781 gcactccacc caaaggcctg tccctgtaat gcggtgcgac ccactcccgt tgaaacggga
    24841 tccccctgcc gaaggcggta ctggcctcga ccaaaagaat caccttgcgg ggatcccggc
    24901 agtattggga ttccagaaag gcattcaagg caaggtcctc gggaatcgag gtggaggaga
    24961 aacggatctt caagccgtcg caatgatccg gagagaaatg cctgtcccta ttgacggcgg
    25021 ttgccgttgc ggtgacgagg ttaaattcgc agtcaaaccg acctcgttgc tgttggaacc
    25081 attcgcacaa gacgaaatcc agggaagcag ccgaacccga aaaacagggt cccaaaatcc
    25141 ggatgttcat gtaggtttgc tgtctgccgc aaaaccgacc tgacccgtcg ccgcccagac
    25201 aatctccctt tccaggcgat tgcaggggcc tcaaatcgga ctcgaagacc atccctcggc
    25261 gttcgaactc cgcaatctga tcgagagcat tgcggagggc tttcttgtgg attcccgtgg
    25321 tgggggtttc ccctaccagg aaaaggagca gcaaatcctt gttctcgcat cggtcgcagc
    25381 ggaacagcat gatccctgct tcttcggcaa atcgtcggga actgagggcc tgcttcctgg
    25441 cggcttcatc ctgcgtggaa ttcggccagg gaaggtagaa acgatccagg tcgaaacccg
    25501 ctctttccac ggcccgctga acagccgcca gatagtgatc gaagtaggga atatgggagt
    25561 cgaagggatc cgggaccatg gcaatcagga atcggacttt gtggccgtcg gcagggtgtt
    25621 tgcggcaagg gtccgaactt tggggtttcc ttccataccg gaattcttca atcaaatcga
    25681 aggatcgccc ttggaaggcc gtcatggtgg gaagtcggtc tgcctcctgg gaaacggttg
    25741 cactggctgc ctcttcttct gccgcacctt gcgacgaggg tcctgcgtat ccgcacaaac
    25801 ccaaaacaag cgctgcaaga acggctacgg gaatcataag accgaaaggg ctgcgattct
    25861 ccattgtgcc caattccttt ataatcagga cctacccggc gactcatatt catgaccggc
    25921 aggattcccc ggccccaccg gccggattca tgccgcttcc ggaagacaag ggcggatcga
    25981 gcctgacgcg ggtcgtactc cttcgtcacc cttggggcaa ataaagggcc tagcctcgcg
    26041 gcggagcccg gaggtccacc cattcgtcct cccggctccg ttgaaactcg tggttttcca
    26101 aacggggaca atcccaatgg aacgcttcaa gaataacgcc catggcggcg ctcctccctt
    26161 tctcggatgg tttcggttcc caaaccccgg ctccgaaaga ggtccacgcc gggaacctcc
    26221 aaccggcccg atccataggg aaaagtatcc gtagaacgca acaaaccttg gcttcctctt
    26281 catgtccgac ctcccgaaat ccatccgccg tcaccctcgt tcaccgtccg atcttttcct
    26341 ttttcggatg ttgacccgta caccatcctt gaagtaatgt tgcgctgtgc agggtggtgt
    26401 acgtgttcac catggatacc tatacacacc cgaatggtca tagtcaagac gaaagatgac
    26461 cttttgggca cttccgcaaa tgattttttg tctaacctgc ccaattggtc gcaatcgtgg
    26521 attatttcaa tgacttggag tgccagttcc gcaaattctc ttggttccga atttacttcc
    26581 gcaatttgat tgtttttgcg gaattaaatc cgatccccaa cggacgacca aacggtgttg
    26641 aagtcaagaa tcagaattgt caggggcgac ctcacgcagc aagctttcgc ggaccaactt
    26701 gatatccact ttcgtacggt ccagcgctgg gaagcgggca caaggcttcc ctgtgctgat
    26761 gatctagtca agattcagca agagttcggc attaacatta actggcttct gaccggcact
    26821 ggaccaaagt atttaagaga tcttgcctgc cccgaaccag agcttaaggt ttgccggatc
    26881 gaggatgctc caatcgagcg aatcaaatca tggattcaag agatagtgga agaacagcca
    26941 gcgtaccgag gatggcttga cattgaattt ccaaaacgct tcccggagtt taaggagtgg
    27001 cttgaaaaaa aacagagggg ggagtcactt gagaatagta gtcccatgac ccacgagaac
    27061 agcgcatagg ggcgctcttg ttgggaaaat ttggttcgca atcgagtttt tcgtgttttt
    27121 aggcgcagac tcttatatat tcatggatgt tagcgtctta cgcttcctgt tcgcaaaaat
    27181 caggtagttc gcaatttggt tcgcaatcgg agtgaataat tgcgaaccag gtagcaacag
    27241 aaggatcgcc ttaaacattc atacaaaagg aggtatagaa atggctctgt ctaaatgtcg
    27301 tgaatgtggc aatcaggtga gcaccga
//
LOCUS       DAOOVC010000045        26079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1261_length_26079_cov_6.261220, whole genome shotgun sequence.
ACCESSION   DAOOVC010000045 DAOOVC010000000
VERSION     DAOOVC010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 26079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26079
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1261_length_26079_cov_6.261220"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLM79_07945"
     CDS             <1..247
                     /locus_tag="PLM79_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86280.1"
                     /translation="GRLAGEVELLTARRVRAAARVDRGDAAAVLAQRAREVRADLVVL
                     AVRGLAGLSAFWADAIPRKIAGSYEGALLLLRRVEAG"
     gene            363..1853
                     /locus_tag="PLM79_07950"
     CDS             363..1853
                     /locus_tag="PLM79_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86281.1"
                     /translation="MTGSRTLRGKMVVLTVSLGTALLFVAGCGLWLGYQSRAAVSFST
                     DKSLKALQGVRELVMSLSSQKEILTWYLLWGTPENLDRLKQESRTFEETLKKVRETVS
                     TPSQREQVNEIESLYIRYSYSRDQAAALRKSDGIGPLSPLPPVLEHQLKDMASRVQGL
                     AGSLEARVLEWNAGLESRLRLTRQLFYGGILLCAVLLGCMGYFVFRKILGPVRHLALT
                     ASPGLRETDVRDEIKELGRRLYSLMRDVDETQTELQQSREQLFQSEKLAMVGKLAAGV
                     AHTIRNPLTSVKMRLFSLGRTLSLNPVQREDFEVISEEIRHIDTIVQNFLEFSRPPKL
                     KIQKISPSDVVDMAIQLLRHRIESYGVEVELYRQRRLPSIEGDPEQLKEVLVNLIINA
                     CEAMGDGGRIVIREEEGFAEPLGRVVVIRVGDNGPGMPESIHERVFQPFFTTKEGGTG
                     LGLSIAARIIEDHRGFLKLKSREGKGTTLTITLPCKEGEAWLRSLS"
     gene            1832..3274
                     /locus_tag="PLM79_07955"
     CDS             1832..3274
                     /locus_tag="PLM79_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86282.1"
                     /translation="MATIFVIDDDPQLRQSFERLLKQEGHTVRTASTAETGIAMIRDG
                     APDLVIMDVRLPGMSGLEAFSVIHEMQPKLPVIIMTAFGTTETAIEATKMGAFDYVLK
                     PFDIPEILLLIQQAVEAGRFMRSRVEMDASPAGSTEPAEVIIGRSKPMQELYKAIGRV
                     APTDATVLIRGESGTGKELVARAIYQHSLRSERPFLVINCVAIPESLLESELFGYEKG
                     AFTGAANRRVGRIEQAQGGTILLDEIGDMPFGIQAKILRLIQEKSIERLGGREPIPVD
                     VRIIGATNRDLESALEEGRFREDLYYRLKVVTLWLPALRDRTGDIPLLADYFLETFAR
                     AMEIPNPGITEAARDFLCGHSWPGNVRELANTIQKALIFSRGCPVRPEELSQAIGIDP
                     AFRGPDQEISAELVRRWVRHMLMREDHESLFETFLDRFAGMVIREALNLTGGNRTRTA
                     KLLGLSRPTLLSKIEKYRIKIETTAQSDSN"
     gene            3682..4935
                     /locus_tag="PLM79_07960"
     CDS             3682..4935
                     /locus_tag="PLM79_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV86283.1"
                     /translation="MSFSRQVFEFLKAASAAHARWDLEVSLSILRSRKKLLVLFLLLL
                     PILGVSFLEAAPMLGSKTAYAPAFYSTTIFLVSIAIGLAAGLITGCIGAGGGFIITPA
                     LMAAGVKGILAVGTDLFHIFAKAIMGTAVHKKLGNVSVKLAVAFLVGSGVGTFIGGAI
                     NKGLYNKDPLLSEAFISAVYAILLGFLGFYALSDFLRSSKGGGGGGDAHGGGPVGTPP
                     MALKLQNLNLPPMIQFDEDFVPGGKRISGAIIAAGGVVVGIMAAIMGVGGGFITFPMF
                     VYIFGVSSMTTVGTDILQIIFTAGLAAVGQYAIYGYVFYTLAMGMLIGSLLGIQVGAL
                     TTKVVKGIHIRGFYAVSILAGFINRAATLPKKFTELEVLDLSKSLVSNIEFVGNIIFW
                     VVVAAFGVWVFGKFFANLGELREEE"
     gene            4938..5648
                     /locus_tag="PLM79_07965"
     CDS             4938..5648
                     /locus_tag="PLM79_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86284.1"
                     /translation="MLIIHKKPFNMGALLGVSFFMVLFLIFSPLFGGKNGLEYSDDLF
                     NKLSKGSSYFIPKLTEEVKKFSGKNIGVTLKLDKPEVVEKTAKVLMVAGAQVGVQGSE
                     VRIGADLGKMLSVVIKDSDAMYHNDAQKVAAAYGLEATEVMSLWWGVLTKMDKELKKD
                     KKVEESNVVSEVTKKGIEPAFNFFGIEAQRVSDKAGTMIGLLVFYVLYTMWWGYAIFY
                     MFDGIGLSMKKAKVKKEV"
     gene            5659..6108
                     /locus_tag="PLM79_07970"
     CDS             5659..6108
                     /locus_tag="PLM79_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86285.1"
                     /translation="MKLLVPVDGSTHSMEAIKVAMDYAKMKGAEINVISVVPYIGGME
                     DHEISPSRRDRHMESIEKMADEAVRKACDLLAQEQITSSCSRTIVTSISVPDAIVEFA
                     EKEKVNLIIMGSRGLSASSGFRIGSVASQVAKASPCSVYLVKVAQES"
     gene            6241..6651
                     /locus_tag="PLM79_07975"
     CDS             6241..6651
                     /locus_tag="PLM79_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86286.1"
                     /translation="MNEAFSILVTDKNGHVREFLRRELHEDGYRVYTVRDGRDLLSLL
                     EGETRLDLLILDLEIPYADESLILEQVLERRADLPVVIHTHTPEALDSGHYASALAIV
                     EKSGDTDNLRRVVRETLQHLFPRRFASIPPSRPR"
     gene            6889..7305
                     /locus_tag="PLM79_07980"
     CDS             6889..7305
                     /locus_tag="PLM79_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86287.1"
                     /translation="MEEIKVLVVDDETEFLETLVKRMKKRKLDAKGAQSGEEGLEVLK
                     QSPVDVVVLDVKMPGMDGIETLREIKKSFPLVEVIMLTGHANLEVAIEGMELGAFDYL
                     MKPMDIDELLYKVQDAYKRKALHADRIKQVEAVMGS"
     gene            7950..10577
                     /locus_tag="PLM79_07985"
     CDS             7950..10577
                     /locus_tag="PLM79_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV86288.1"
                     /translation="MGKISQWLSKIGLKTKGRALSVAEAEQLRAAFRARYHAFKLLLS
                     ANNKALEIMADMEKALKGSLPFGIAFVRANCTAVGVNVYRIIRNLDQLAPGKYQDLYD
                     RFKEIQEDINQELSRRKEPSGDRLVIFFDDIDKEMADQVGSKMANLGEVRNRLSLAVP
                     NGFVVTARAYRKFFECNDLQTEIDRRLQTFSGEEVDQLYSLSADIQQLIIRSPVPREV
                     EEAIREAYGKLEEEAGPGVRVSMRSSALGEDAAGASFAGQYRSELNVAPDNLIQAYKE
                     IVASKYSLQAISYRLNRGILDEDISMCVGCMVMVPAVVGGVTYSRNPLNIRDDSILIH
                     SVWGLPKSVVDGSVASDVFVVGRNPSMEILERDIQQKDYKFVCYAEEGVCRVELSGEA
                     ATRASLTDEQVLDLARMAARLEEYYGSPQDVEWALDEAGRLCTLQCRPLKQTEVPASQ
                     TSRMGEEVAEGDVILEGGATASPGVACGPVFLVRRNSDVLQFPQGAVLVTSQALPRWA
                     ALLNRTCAVVAEQGSVAGHLANVAREFGVPALLGVRRATDLLRTGETVTVDADGLRIY
                     KGRVDSLLRTARSKKNLMLGSPVYEVLEEVSKHIIPLNLLDPDSTDFRPKKCRTLHDI
                     TRFCHEKSVHEMFSFGKEHRFSERASKQLICDIPMQWWFINLDDGFREDVPGRFVTLD
                     NIVSIPMLALWEGIVAVPWEGPPPVDAKGFMSVLMEATANPALDPSMPSPYVNRNYFM
                     ISKNFCTLSSRFGFHFSTIEALVSDRAQENYVSFHFKGGAADAQRRIRRACFLAGIVE
                     EFGFRVEVKEDGVFARIEGYEEEVMKEKLKILGYLIIHTRQLDMIMSNDFSYNQHRTK
                     IMNEIRRILPGKAESPGSA"
     gene            10678..11646
                     /locus_tag="PLM79_07990"
     CDS             10678..11646
                     /locus_tag="PLM79_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86289.1"
                     /translation="MASPNGKRVLVAIDGSDQALQAARYAGTFFSPGRAEIVLFHVYT
                     RIPESFWDMEKEPAFHYKIVNIQAWEDRQEEVARELMVRAKQELLAAGVGEECIRLLS
                     VPRKAGVARDIIEEAQSGYDALVIGRSGLSQLKDFMLGSIADKLVEKLTHLPTWVVGG
                     RSLPGRLLLSLDASEGAMRAVDHVAQMAVGYPYVQVTLFHAIRRLDVFFHGLGDAYAV
                     GYENEWKEIARKELEEAAREMESTFDQARRRLLDAGLKDDQVRIRMVREVSSRAHAIV
                     EEADREGMDTIVVGRRGLSRVQEFFMGRVSNKVIQLAKDKTVWVVN"
     gene            11727..13148
                     /locus_tag="PLM79_07995"
     CDS             11727..13148
                     /locus_tag="PLM79_07995"
                     /inference="COORDINATES: protein motif:HMM:NF037415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86290.1"
                     /translation="MTELEKVLHRVVDRVNVNLRELSMDVGPFVRTLVVPENFVRFYA
                     FYGFTPHHPLSFQFRRSSLAGSYFLGKCVVDHSIVYKSDVRGDELKTKGETLKCGSLE
                     IPLHEDEMIRIKDSFLVKTLVHSNSHDPESPEEFLIQNTVALHYANIHGSPIEGSFLG
                     PFGTVDLTTVHDCVIGSFAYVQRGELAHARIDPGRIWVQAGDVFEFNYQYPMEVLEKY
                     IHLEAGKKPRGKLVDFVEGRKKDYEEIFGLVGKELLDSLPKGTKVSRYAVVKGKNRIE
                     ENVLVAQRAYIEDSRLGRGSNAQENCYIVQSELEGNDVTAHGGKVICATLGNKVFVGF
                     NSFLHGTPSCRLTVGEGSIVMPHTVIDLEQPLEVPPKTLVWGYLRSPEDLASQTIPLE
                     KLAHHKGELQLGSMYFRGSGHEFVHGFQHRIEHILEANGAFFDGNAHPGHAQKTRNIS
                     YNTIQPYPDGPLKGLYPTIEIRP"
     gene            13349..14413
                     /locus_tag="PLM79_08000"
     CDS             13349..14413
                     /locus_tag="PLM79_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86291.1"
                     /translation="MKIGLVFLLLIPGMVLGPAAAAPAEESVTVYSVPQSESAKAAEY
                     WTPERMRRAKPRKMPVRPEKPASTQASGEEEIRGEPGMDPGGMPGEKSPKRRSKPAME
                     PEVQKADATAEGYKYPPPQNTFAVPKEYYKTYPYMAVGKVFFVEDGEDNVCSASSIGG
                     NAVITAGHCVSDGKGHFLEKWVFVPAYIKEGKTPVKPYGTWPATDFILFGSYHKGGDI
                     GRDVAFAVTKNNDQGEKLSDVVGYLGFAYNKSRVQHWNMFGYPAVEPFNGRILYQSQA
                     SYADKDGSVEPPTMGMGTTQTGGCSGGPWILEFTPLNDTDGNFVFGVNSYVYGNQPKL
                     MYAPYFDDEVKRLKDRAVAK"
     gene            complement(14388..14882)
                     /locus_tag="PLM79_08005"
     CDS             complement(14388..14882)
                     /locus_tag="PLM79_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOV86292.1"
                     /translation="MVPRLERNGAGGNGGRSGAFDRIFHHLCGRLPPREGRRTAVPGP
                     GGGRGLPERGPIGVMLAEHNAGRGYVRALRSALADLDRGNRSVLPEVERQAEGYVRLL
                     REHMEKENRVLFRIAEDRLTRDVKDCLKAGFERIEAERVGNGRGSGEISREGRSLTWL
                     PPGP"
     gene            complement(14821..14952)
                     /locus_tag="PLM79_08010"
     CDS             complement(14821..14952)
                     /locus_tag="PLM79_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86293.1"
                     /translation="MNPIEELRQEHEGLRLSLEILFPDGSKAGKERSWGKRRKIRGV"
     gene            complement(15199..16524)
                     /locus_tag="PLM79_08015"
                     /pseudo
     CDS             complement(15199..16524)
                     /locus_tag="PLM79_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
     gene            complement(16857..17351)
                     /locus_tag="PLM79_08020"
     CDS             complement(16857..17351)
                     /locus_tag="PLM79_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86294.1"
                     /translation="MRDNKGLWITVGLVIFAAFFVLGWFGREVYRNVPPIPERVATHS
                     GEILLTREDILRGRQGWQSIGGHQVGSIWGHGAYQAPDWSADWLHREATALREITNLE
                     LYGRSFGELSAAGAAVGEQVKVALRTNTYDPSSGTLEASRERARAMAETAGHFRALFG
                     GDRP"
     gene            17548..19110
                     /gene="norR"
                     /locus_tag="PLM79_08025"
     CDS             17548..19110
                     /gene="norR"
                     /locus_tag="PLM79_08025"
                     /inference="COORDINATES: protein motif:HMM:NF003451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HOV86295.1"
                     /translation="METFDTLITVALDLTAALSAGDRYRRLLEALGGVIPYDAASLLR
                     LEGEDLVPVAARGLSADALGRRYPLDRHPRLARICRASEPVRFSADDPSPDPFDGMIR
                     GDPSAARRVHACLGCPLYVEGELIGALTADALDPTAFQGLDQKFLRAIAAMAAAQMRA
                     ADLMEALENSAVRQGMIARDLMADLHRGKEILGSSLAVEHLRREIGLVAGSDFTVLVL
                     GETGVGKELAVRAIHAASLRRNAPLLYLNCAALPEGLAESELFGHTKGAFTGAVRDRP
                     GKFEVADGGTLFLDEIGELPLSIQPKLLRAVQEGEVRRIGSGRTVRVNVRLLAATNRN
                     LEEEVREGRFRADLFHRLSVYPLVVPPLRERREDIPLLAGHFCEAIQRRLGLGPVRLD
                     SDALGLLRRYPWPGNVRELENVLSRAILKAGAQVPRGKLVVVAPVHLGGDLEAAPGIG
                     DPSPPRETLRVSAGRSFREVVEDFQKDLILQTVAGNRGNWAAAARELGMHRSNLHKLA
                     SRLGIRNPGAKR"
     gene            19294..20937
                     /locus_tag="PLM79_08030"
     CDS             19294..20937
                     /locus_tag="PLM79_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV86296.1"
                     /translation="MSRVAGDQSISRRDFMKTAAALAAGMTARWDVLEALAAGIPKGD
                     LPVVVIGAGLGGLSAAALLARNGFPVTLVEQHEKPGGYATTFDRAGGKFTFDVSLHLV
                     SGAKNWLGPIFEGTGIRDKIQMVQLPELCRIVAPHHDFLWPNHPDAAIDALIELFPTE
                     ALGIRGFFAQMLGLADEIMVPFSPDSILDRVFFPLTHKRMWALRNKTLAEVLDAHVRD
                     PQLRGLLSAYWGYYGLPPSRLSGFLYTVATGSFLRFGAHYIKRRSQDLSDALLEAVET
                     AGGKVLLETEAVGIEMRGGKIAGVRLEGGTLLPAGAVISNAGVPSTVKMLPSGALPGD
                     YLRKLEGYRPSLSTFCVWLGLNGEIRDRVKGYEVFVLRDYDPERAFRASLVCDPAAGS
                     LAVTLYDNCYPGYSRPGTSTVSILMSCAYEPWRRFEADYFAGRKDAYRKEKERIARVL
                     VEETERRVIPGLTSMIEVMEASTPLTNLRYTRNPEGAVYGYEQTLSNSFMTRLDNRTP
                     LKGLYFASAWTFPGGGYEPCLESGARAYRALVTDWTPRA"
     gene            21149..22459
                     /gene="purB"
                     /locus_tag="PLM79_08035"
     CDS             21149..22459
                     /gene="purB"
                     /locus_tag="PLM79_08035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOV86297.1"
                     /translation="MIERYTRPEMGRIWTLENKYRRWLDVEIAVCEVLAEEGRVPAQD
                     LKEIKNRAGFDVKRIDEIEQTTHHDVIAFLTSVAEHVGPASRFIHQGLTSSDVLDTAN
                     ALLLTEAADLLLADLDRLLEVLKAKAFEWKDLVMIGRSHGIHAEPTTFGIKFALWFAE
                     MTRNRDRLAHARESVRVGKISGAVGTFANIEPRVEEKVCKKLGLMPAPVSTQVIQRDR
                     YAEFFATVAIVGCTVEKIALEIRHLQRTEVREAEEFFREGQKGSSAMPHKRNPIASEN
                     LSGLARVLRGNALAAMENVALWHERDISHSSVERIICPDSTILLDYMLNRLTRVLENL
                     VVYPENMRKNLDLTGGLFYSQQVMLALTGRGISREEAYRLVQRNAMKVWNQGGTLQER
                     LREDAEVMALLPAGIMDGLFDLKHHLQHVDTIYQRVFGSHESSS"
     gene            22443..24566
                     /locus_tag="PLM79_08040"
     CDS             22443..24566
                     /locus_tag="PLM79_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOV86298.1"
                     /translation="MNPLPELLAPAGGVEGLVAAVENGADAVYLGLKEYSARASAANF
                     TVEELAVLLPYAHGKQVRIYVALNSLLGSSDLPKVLDQLQALSDLKPDGIIVQDPAVV
                     HFVRRYFPDLKLHASTLMTIHNSAGANQLQRMGFQRVVLARELTLEEIEGITANTSVE
                     LEIFVHGALCYSYSGLCLASSFRGGRSGLEGRCVQPCRLRFRQGRKEGFFLSCNDLCA
                     LPLLSRIKGMKLSALKIEGRLKSADYIGRVVRAYRIVLDASPEREKEALGEARAMLSL
                     APSRRLTTGFLEDSRGRDVLTPHRGGSSGLWVGTVSSVTAGAVTVLLRHEIRTGDRLR
                     PDSSRGEEKEAFTVIRMRGERGEELVRGEAGERVHLEVQGHFAPGERLFRIGSQGPPP
                     ASLWQRIRSEGRESARFRRKFPRSRDALEELDQGQGEEQRGTTREVVFLKIASLRDLG
                     AAFQIENVRVVLPATRSNLEQLAKRKLSAQQKRRFVWSLPPLLWESDLEYTRAAVAWY
                     RARDYQTWEWNNWGHLDLFAGREHVRLVAGSRLNVKNGAALAALLENGCGACVLSPEI
                     TREELLHLGAHGPRVRMIVPVYSWPPLFSSRLLPKLDTERPLLTPRAEPLFFRTDPRG
                     TSFLYADRPVNWLAQVPFLRANGFSRFLVDLSDGPNPEAHTLTRVWEDFRRARATGPH
                     SLFNYDRQTSRGKRGPRRKGSERNR"
     gene            24563..25816
                     /locus_tag="PLM79_08045"
     CDS             24563..25816
                     /locus_tag="PLM79_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV86299.1"
                     /translation="MIEWLRVYGNRRVLMLLGLGFSSGLPLALTGSTLQAWMTVEKVD
                     LRVIGIFSLVGLPYTLKVLWAPVMDRFTLPFLGRRRGWIGLAQVLLALGLVGLSFSSP
                     ASMPWLVAFLALTVAFLSASQDIVIDAYRADVLREGELGAGAATTVVGYRLALLVSGA
                     LALILSDHLSWSHVYILMAVLMGGGLFFTLGAPEPEEKVVAPRSLREAVWEPLVTYFH
                     RSRAVEMLLFIMLYKLGDAIAGAMTTPFLLDMGFSRTDVGMANKALGLITTIVGTLAG
                     GGIISKLGIHRSLWIFAFLQAGSNLTFTALAVAGKNYPIMVGAVAVENLCGGMGTAAF
                     IAFMMSLCDKRFTATQYALLTSFMAVTRVVAGVPTGFMVNSLGWPMFYAVSVLGALPG
                     ILLLPRFAPWRSVPERKEPALQGTR"
     gene            complement(25831..>26079)
                     /locus_tag="PLM79_08050"
     CDS             complement(25831..>26079)
                     /locus_tag="PLM79_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86300.1"
                     /translation="PPFLVASTLLVPGYVGEDEVRGIASFIAELDPDIPYTLLAFAPQ
                     FRMNDFPTTSRDHAESCLEAARSAGLRRIRIGNTHLLR"
BASE COUNT         5105 a         7769 c         8226 g         4979 t
ORIGIN      
        1 gggacgcctg gccggggagg tggagttgct caccgcacgc cgggttcgag cggccgcccg
       61 ggtggatcgg ggagatgcgg cggcagtcct ggcccaaagg gctcgagaag tccgggcgga
      121 cctggtggtt cttgccgtga ggggcttggc gggactcagc gccttctggg cggacgccat
      181 cccccgcaag atcgcgggtt cctatgaggg tgccctcctg ctgttgcgac gagtcgaggc
      241 aggataaagg caatgacccg ctttcgacgg gggatgaaaa acagagcccc ggaatccggg
      301 cggacctttc ccgtgccgta cctgggagga gtccgttgcc gggggaaagg gtgggattgg
      361 gcatgacggg ttcaaggacg ctgcgcggca aaatggtggt gctcacggtc agcctgggaa
      421 cggccctgct gttcgttgcg ggctgcggcc tgtggctggg gtaccagtcc cgagcggcgg
      481 tttccttttc gacggacaag agcctgaaag ccttgcaggg ggtacgggaa ttggtgatgt
      541 ccctatcgtc ccagaaggaa atcctcacct ggtacctgct atggggcact cccgaaaacc
      601 tcgatcgcct gaaacaggaa agccgcacct tcgaagaaac gctgaagaag gtgagggaaa
      661 ccgtgtccac gccgtcccag cgagagcagg tgaacgagat cgagtccctc tacatccgtt
      721 acagttactc gagggaccag gctgccgccc tgcgcaaatc cgacggtatc ggtccgctct
      781 ccccgttgcc tcccgtcctc gaacaccagc tcaaggacat ggcgtcccga gtccagggtc
      841 tggccgggtc cctggaggcc cgggtcctcg agtggaacgc cgggctggag agccggctcc
      901 ggctgacccg gcagctgttc tacggcggga ttctcctttg tgccgtgctc ctgggctgca
      961 tggggtactt cgtcttccgg aagattttgg ggccggttcg acacctggcc ctgacggcga
     1021 gcccgggact ccgtgaaacg gatgtgaggg atgaaatcaa ggaactgggc aggcggctct
     1081 acagcctgat gagagacgtg gacgagactc agacggaatt gcagcagagc cgggagcagt
     1141 tgtttcaatc ggagaagctt gccatggtgg gaaaactggc cgccggggtg gctcacacca
     1201 ttcgaaaccc tcttacctcc gtaaaaatgc ggcttttttc cctgggaaga accctgtccc
     1261 tgaacccggt tcagagggag gactttgaag tcatttccga ggaaatccgc cacatcgata
     1321 ccattgtgca gaacttcctc gagttctccc gcccccccaa gttgaaaatc cagaagatca
     1381 gcccgtcgga cgtggtggac atggccatcc agcttcttcg gcaccggatc gagtcctacg
     1441 gggtggaagt ggaactgtac cggcaacgga ggctcccttc cattgaaggc gaccccgaac
     1501 agctgaagga ggtcttggtg aacctcatca tcaacgcctg tgaagccatg ggagacgggg
     1561 gaagaatcgt gatccgggag gaagaggggt ttgcggagcc cctggggcgg gtggtggtca
     1621 ttcgggtggg ggataacgga ccggggatgc ccgagtccat ccacgagagg gtgtttcagc
     1681 ccttcttcac caccaaggag ggaggcacgg ggctgggcct gagcattgcc gctcgaatca
     1741 tcgaagatca ccggggtttc ctcaagctca agtcccggga gggaaaaggg accaccctca
     1801 ccatcaccct accgtgtaag gagggggaag cttggctacg atctttgtca tagacgatga
     1861 tccccagttg cgccagagtt tcgaacgtct tctcaagcag gaaggacaca cggtccgcac
     1921 ggcctcgacg gcggaaaccg gcatcgccat gattcgggac ggggcacccg acctggtcat
     1981 catggacgtg cggcttcccg ggatgagcgg cctcgaggcc ttttcggtca tccatgagat
     2041 gcagcccaag ctgccggtca tcatcatgac ggccttcggg accacggaga ccgccatcga
     2101 ggcgaccaag atgggcgcct tcgactacgt gctcaaaccc ttcgatattc cggagatcct
     2161 cctgctgatt caacaggccg tcgaggccgg tcgattcatg cggtcccgag tggaaatgga
     2221 cgcctccccc gcggggagta ccgaacctgc cgaggtcatc atcggccgca gcaagcccat
     2281 gcaggagctc tacaaggcca tcggacgagt ggctccgacg gacgccacgg tgctcatccg
     2341 cggagagtcg ggaaccggca aggagctcgt ggcccgggcc atttaccagc acagcctgag
     2401 atcggaaagg cctttcctgg tcatcaactg cgtggccatt cccgagagcc tcctcgagag
     2461 cgaacttttc ggctacgaga agggagcgtt cacgggggcc gccaaccgtc gggtgggccg
     2521 gatcgaacag gcccaggggg gaacgatcct tttggacgaa atcggggaca tgccctttgg
     2581 aatccaggcc aagatcttgc gcctgattca ggaaaaaagc atcgagaggc tgggaggacg
     2641 cgaaccgatt cccgtggacg tgcgcattat cggggccacc aaccgggacc tggaatccgc
     2701 cctggaagag gggcgttttc gcgaggacct ctattatcgt ttgaaggtgg taaccctctg
     2761 gctgcccgcc ctgagggatc gcacgggaga cattcccctc ctggcggact acttcctgga
     2821 gacctttgcc cgggccatgg aaatccccaa tccggggatc acggaagcgg cccgggattt
     2881 cctgtgcggg cattcctggc cgggcaacgt gcgggagctg gccaacacga tccagaaggc
     2941 cctcattttc agccgaggct gtcccgttcg tcccgaagag ctgtcccagg ccatcgggat
     3001 cgatcccgct ttcaggggtc ccgaccagga aatcagcgca gaactcgttc gacggtgggt
     3061 ccgccacatg ctgatgaggg aagaccacga aagcctgttc gaaaccttcc tggaccggtt
     3121 tgccgggatg gtcatccggg aagccctcaa tctcaccggg ggcaatcgga ctcggaccgc
     3181 caagctcctg gggctgtccc ggccgacctt gctatccaag atcgaaaagt accgcatcaa
     3241 gatcgagacc accgcccaga gcgactcgaa ctgaccgttt ttacagattg taaaattccc
     3301 tgacaccggg ccggggtcga ggggagatcc ggcttctccc gggttccggc ccattccatt
     3361 tccccgcctt cctttgtgcc caattccaca agatccttcc cacttggcat gcccattgcc
     3421 atggcttctc accggttctt cctttcggcg tgccccggta tccatagcct tccatcggga
     3481 gatgagcggc gggccgggat tcccgacccc ggcgaattgg acccgtggtc gtcgccggga
     3541 ggccgtccgg gcagggccgg ggtcttttgg ttgcggggaa aaggggcaac ggcgaggggc
     3601 gatgcgagta aaggtgtttt ggggggtatt tggaatcatt cgtcatgaag agaaagagtt
     3661 tgttggaagg aggtccgatc gatgagtttt tcaagacagg tgtttgagtt tctaaaggcc
     3721 gcttccgcgg cccatgcgcg atgggatttg gaggtctccc tctccatcct gaggagccgc
     3781 aaaaagttgc tggtgctttt tctgctgctg ctacccatcc tgggcgtctc ctttctcgag
     3841 gcggctccca tgctgggaag caagacggcg tatgcgcccg ccttctattc gaccaccatc
     3901 ttcctggttt ccattgccat cgggttggcg gcaggcctga tcacggggtg tatcggcgca
     3961 gggggcggtt tcatcatcac ccccgccctc atggccgccg gggtgaaggg aatccttgcc
     4021 gtggggacgg acctcttcca tatctttgcc aaggccatca tggggaccgc cgtccacaag
     4081 aaactgggaa atgtgtccgt aaaactggcc gtggccttcc tggtgggatc cggggtgggg
     4141 accttcatcg ggggcgccat caacaagggg ctctacaaca aggatcccct gctcagtgaa
     4201 gccttcatca gcgcggtgta cgccatcttg ttgggatttc tcggcttcta cgccctttcg
     4261 gatttcctac gttccagcaa gggcgggggc gggggagggg acgcccatgg gggcggcccc
     4321 gtcgggactc cccccatggc cctcaagctc cagaatttga accttcctcc catgatccag
     4381 ttcgatgaag acttcgtgcc cgggggcaag cggatctccg gggcgatcat tgccgcagga
     4441 ggcgtggtgg tcgggatcat ggccgccatc atgggcgtgg gcggcggttt catcaccttt
     4501 cccatgttcg tttacatctt cggggtttcc tccatgacca ccgtgggaac ggacatcctc
     4561 cagatcatct tcaccgccgg actggccgcg gtgggtcaat acgccatcta cggctacgtg
     4621 ttttacaccc tggccatggg catgctcatc ggttccctcc tggggatcca agtgggggct
     4681 ctcaccacca aggtcgtcaa gggcattcat atccgaggct tctacgccgt ctccatcctg
     4741 gcagggttca tcaaccgagc cgccaccctt cccaagaaat tcacggaact cgaggtactg
     4801 gatctttcca agtccctggt gagcaacatc gaattcgtgg gaaacatcat cttctgggtg
     4861 gtggtggccg cctttggagt atgggtgttt ggaaagttct tcgccaactt gggcgagttg
     4921 agagaggagg aatagcgatg ttgatcatcc acaaaaaacc gttcaacatg ggagcccttc
     4981 tcggggttag tttcttcatg gtgttgttct tgatcttctc tcccttgttc ggagggaaga
     5041 acggcctgga atattccgac gacctgttca acaagctttc caaaggttcc tcctacttca
     5101 tccccaagct gacggaggag gtaaagaaat tctccggaaa gaacatcggg gtcaccctca
     5161 agctggacaa gcccgaggtc gtggaaaaga cggccaaggt cctcatggtg gcgggggccc
     5221 aggtgggagt ccagggttcg gaggtgagga tcggcgcgga cctgggaaaa atgctctccg
     5281 tagtcatcaa ggattccgac gccatgtacc acaacgatgc ccagaaagtt gcggcagcct
     5341 acggcctgga agcaaccgag gtgatgagcc tctggtgggg cgttctgacc aagatggata
     5401 aggaattgaa aaaggacaag aaagtcgagg aatccaacgt ggtctccgag gtaaccaaga
     5461 agggcatcga gcctgccttc aactttttcg gaatcgaggc tcagagagtc tcggacaaag
     5521 ccggaaccat gatcggcctg ctggtctttt acgtccttta caccatgtgg tggggatatg
     5581 cgatcttcta catgtttgac ggcatcgggc tgagcatgaa gaaggccaaa gtcaagaagg
     5641 aagtctaggg agggaaagat gaaattgctg gtgcccgtag acgggtcgac tcattcaatg
     5701 gaagcaatta aggtggccat ggattacgcg aaaatgaagg gtgcggagat caacgtaatc
     5761 agcgtggttc cctatatcgg cggcatggaa gaccatgaga tctctccatc ccgaagggac
     5821 cgccacatgg agagcatcga aaaaatggcc gacgaagcgg tccggaaggc ctgcgacctg
     5881 ctggcccagg agcagatcac ctcgagctgt tcccggacca tagtgacctc catttccgta
     5941 ccggatgcca tcgtcgagtt cgcggagaag gagaaggtga atctcatcat catgggaagc
     6001 cggggcttga gcgcctcttc cgggtttagg atcgggagtg tggcgagcca ggtggccaaa
     6061 gccagtccgt gttcggtgta cctggtcaag gttgcccaag aatcctgagg acgttgcgaa
     6121 tccccccgga cggaaaggat cgtatccttc ctccggggaa gacccttggt tcctccgcag
     6181 ggcaaatgtc ggttttgggc gggactttcg gggaccccag cggaatgcga ggccggtaaa
     6241 atgaacgaag ccttctccat cctcgtgacg gacaagaacg gccatgtccg ggaattcctg
     6301 cgccgggaac tgcacgagga cggctaccgg gtgtatacgg tccgagacgg tagggacctt
     6361 ttgagtctcc tcgaggggga gacccgcctg gatctgctca tcctggacct ggagattccc
     6421 tatgcggacg aatccctcat cctggagcag gtgctggaaa gaagggccga ccttcccgtg
     6481 gtcatccaca cccacacccc ggaagcactg gacagcggcc actatgcctc cgccctggcc
     6541 atcgtcgaaa agagcgggga cacggataac ctgagaagag tggttcggga aacgcttcaa
     6601 catctgtttc cacgacgttt tgcctccatc cccccgtccc gtccccgata agcccccgga
     6661 agaggtcgtc cccctcaaga gcttttcttt ttggctccca gcctcccggc cccccagcct
     6721 cccggcttcc cgggtccaaa agagggaagt cggttggaaa acctttgatt ttccatccgt
     6781 tttaaggaaa aattccgtgt gtgccatggg tgcttctaaa cgagttccac gagccggaat
     6841 cggaaagacc catccgaggg gaccgggggt gaagagaagg gggccaaatt ggaggagatc
     6901 aaagtcctgg tggtggatga cgaaacggaa ttcctggaaa ccttggtaaa acggatgaag
     6961 aaacgcaaac tggatgccaa gggggctcag agcggagagg agggcctgga agtcctgaaa
     7021 cagtctcccg tggacgtggt ggtgctggac gtgaagatgc ccggcatgga cggaatcgag
     7081 accctgaggg aaatcaagaa gagtttcccc ctggtggagg tgatcatgct gaccgggcac
     7141 gccaatttgg aggtggccat cgaggggatg gagctgggtg ccttcgacta cctgatgaag
     7201 cctatggata tcgatgaact tctttacaag gttcaggacg cctacaagag gaaggccctt
     7261 cacgcagata ggatcaaaca ggtcgaggcc gtgatgggtt cctgactttc caccgccgtc
     7321 cggtctaccc ccatatgccc gtcccggcgg gcatgcaact tcttgtctcg gctttcctca
     7381 agggtgtgcg ggaccggcgc cccgggggcc ctgatccttc tctaaatgga gcgattcgcc
     7441 gttagcccgt agctgtcggc tctttggcct gtatctcaaa atcttcggac gtcccctgtc
     7501 gggtggtggc gacagcagcg caactacagg aaatctcgaa gctaatgggg aaaccctaag
     7561 cggatcaatt ccgagagcgt cagggcttca tgcccgtgaa ggcgggcatc cggagggtct
     7621 gcaccctccc catggcttcc cgcttgcgcg ggaatgacaa aaggggattg gtcccagcca
     7681 aatcgctcaa agcaggtaaa ttcgaaaggt gaatcgctcg atttggatcc ttgattttgt
     7741 ttatctttat cccgaccgga agccgtcccc cgggtttggg tccatggcgc cgggacgggt
     7801 tcccgtgccc atggggctaa cgactgatcc ctcagtgtag atctcggttc gtggaaaggg
     7861 gaaatcgcca cgcagtttcg atttctccac tggaggatgg gccaaaagcc gttgcatatc
     7921 cttgttttcc ccggactgcc agattggtta tgggcaaaat atcccaatgg ctcagcaaga
     7981 tcggcctcaa gaccaagggg agagcgcttt ccgttgccga ggcggagcaa ctgagggcgg
     8041 cctttagagc tcgctaccac gccttcaagc tcctgctgag cgccaacaac aaggccctcg
     8101 aaatcatggc cgacatggaa aaggccctca aaggcagtct gcctttcggg attgccttcg
     8161 ttcgggccaa ttgcacggcc gtgggggtca atgtctaccg gatcatccga aacctggatc
     8221 agctcgcccc gggcaagtac caggaccttt acgatcgttt caaagaaatc caggaagaca
     8281 tcaaccagga gctctcccgg agaaaggaac cgtcgggaga ccgcctggtc attttttttg
     8341 acgacatcga caaggaaatg gccgaccagg tgggaagcaa aatggccaac cttggagagg
     8401 tgcggaatcg gttgtccctg gcggttccca acgggtttgt ggtcacggcc cgggcgtacc
     8461 ggaagttctt cgagtgcaac gacctgcaga cggaaatcga ccgtcgtcta cagacctttt
     8521 ccggggagga ggtggaccag ctctactccc tgagcgcgga tatccagcaa ctcatcattc
     8581 gatccccggt ccctcgggag gtggaggagg ccatccggga ggcctacggg aaactcgaag
     8641 aagaagcggg accgggggtg cgggtctcca tgcgcagcag cgcactgggg gaagacgccg
     8701 cgggcgcttc cttcgccggg cagtaccggt cggaactgaa cgtggccccg gacaacctga
     8761 tccaggccta caaggaaatc gtggccagca agtacagtct ccaggccatt tcctatcggc
     8821 tcaatcgagg catccttgac gaagacatct ccatgtgcgt ggggtgtatg gtcatggttc
     8881 ccgctgtggt gggaggcgtc acctactcga gaaatcctct caatatccgg gacgattcca
     8941 ttctcatcca ttccgtgtgg gggttgccca agtccgtagt ggacgggagc gtcgcttcgg
     9001 acgtgtttgt ggtgggaaga aacccctcca tggaaatcct ggaacgggat attcagcaga
     9061 aagattacaa attcgtgtgt tatgccgagg agggggtgtg ccgcgtcgag ctgtccggag
     9121 aggcggcgac ccgggcgtcc ctcaccgacg agcaggtgct cgatctggct cggatggccg
     9181 cccggctcga ggaatattac gggtctcccc aggacgtgga atgggccctg gacgaggcgg
     9241 ggcggctctg cacccttcag tgccgaccct tgaaacagac ggaggtcccg gcatcccaga
     9301 cgtcccgaat gggagaggag gtcgccgaag gagacgtgat cctcgaggga ggggccacgg
     9361 ccagtccagg ggtggcttgc ggccccgtgt tcctggtccg gcgaaacagc gatgtcctcc
     9421 agtttcccca gggggcggtc ctggtgactt cccaggccct gccccggtgg gccgcccttc
     9481 tcaaccgaac ctgcgccgtc gtggcggaac agggaagcgt cgccgggcac ctggccaatg
     9541 tggcccggga gttcggagtc ccggccctgc ttggggtgag gagagccacg gacctactcc
     9601 ggacgggaga aacggtcacg gtggatgcgg acgggcttcg catctacaaa gggcgagtcg
     9661 attccctcct gcggacggcc cggtccaaga agaacctcat gctgggcagt cccgtgtacg
     9721 aagttctcga agaggtttcc aagcacatca ttcccctgaa cctgctggac ccggattcca
     9781 cggacttccg gcccaaaaag tgccgcaccc tccacgacat cacccggttc tgccatgaaa
     9841 aatccgtcca cgagatgttc agcttcggca aagaacaccg gttttccgaa cgggccagca
     9901 agcaactcat ctgtgacatt cccatgcagt ggtggttcat caacctggat gacggattca
     9961 gggaggacgt tcccggaaga ttcgtcaccc tggacaatat cgtctccatc cccatgctgg
    10021 ctttgtggga gggcattgtg gccgtgccct gggagggtcc tccgccggtg gacgccaagg
    10081 gattcatgtc ggtgctcatg gaagcgaccg ccaatcccgc cctggatccc tccatgcctt
    10141 ccccctacgt caaccgcaac tactttatga tttccaagaa cttctgcacg ctcagctcaa
    10201 ggttcggctt tcatttctcc accatcgaag ccctggtgag cgatcgggcc caggagaact
    10261 acgtgagctt tcatttcaag gggggggctg cggatgccca acggaggatc cggcgggcct
    10321 gctttctggc gggaattgtg gaggaattcg gtttccgggt ggaggtgaag gaggacgggg
    10381 tgttcgcccg catcgagggc tacgaggaag aggtcatgaa ggaaaagctg aagatccttg
    10441 ggtacctcat catccacacc cggcaactgg acatgatcat gtccaacgat ttttcctaca
    10501 accaacatag aacgaaaatc atgaacgaga tccgaaggat cctcccgggg aaggcggaat
    10561 cccccgggtc cgcctgaggc tcggcccgag gcgccttcct ccgggttgcg gcccgtcatt
    10621 gccggccgcg agagttgcgg gcgaccggga cggtcgggac ggaaagaaag gaaagatatg
    10681 gcttctccca atggaaaacg ggtcctggtg gccattgacg gttccgatca ggccttgcag
    10741 gccgcccgat atgccgggac gtttttctcc ccgggacggg cggaaatcgt cctctttcac
    10801 gtctacactc gaattcccga atccttctgg gacatggaga aagagcccgc cttccattac
    10861 aagatcgtga acatccaggc gtgggaagac cggcaggaag aagtggcccg ggagctcatg
    10921 gtgcgggcga aacaggaatt gcttgccgcg ggagtcggcg aagaatgcat ccggctgctt
    10981 tccgtgccgc gaaaggccgg ggtcgcccgg gatatcatcg aggaagctca aagcggctac
    11041 gacgccctgg tgatcggacg cagcgggttg agccaactga aggacttcat gttggggagc
    11101 atagcggaca aactggtgga gaaactcact cacctgccca cctgggtggt tggaggcagg
    11161 tctctgccgg gaaggctgct gttgtccctg gacgcgtccg aaggagccat gcgcgccgtg
    11221 gaccacgtgg cgcagatggc cgtggggtat ccgtacgtac aggtgaccct gttccatgcc
    11281 atccgtaggt tggatgtctt cttccacggt ctgggggacg cctatgccgt cggctacgag
    11341 aacgaatgga aagagatcgc ccggaaagaa ctggaggaag ccgccaggga gatggaatcc
    11401 actttcgatc aagcccgccg acgcctgctg gacgcaggcc tcaaggacga tcaggttcgc
    11461 attcgaatgg tccgggaagt cagcagccgg gcccacgcca ttgtcgagga agcggatcgg
    11521 gagggaatgg acaccatcgt ggtcgggcgg agggggctct cgagagtaca ggaatttttc
    11581 atggggcgtg tgagcaacaa ggtcatccag ttggccaagg ataagacggt ttgggtcgtg
    11641 aactgacggt cgacagggtt tcaaggggcg gccgtgcgtt catccagtcg agggaaacgt
    11701 tggaccattg gaaaagggag aagggaatga ccgaattgga aaaggtgctg catcgtgtgg
    11761 tggatcgtgt gaatgtgaat ctgcgggagc tctctatgga cgtgggaccg ttcgtccgca
    11821 ccctggttgt tccggaaaat ttcgtccgat tctatgcctt ttacgggttt acgccccatc
    11881 atcctcttag ctttcagttc cgccgatcga gcctcgcggg aagctatttc ctgggcaagt
    11941 gcgtcgtgga ccattccatt gtctacaaga gcgacgttcg aggcgatgaa ctcaagacca
    12001 agggagaaac cctcaagtgc ggttccctgg aaatccctct gcacgaagac gaaatgatcc
    12061 gaatcaaaga cagcttcctg gtaaagaccc tcgtccacag caactcccat gacccggaaa
    12121 gccccgagga gtttctcatt caaaacaccg tggccctgca ttatgccaac attcacgggt
    12181 cgcccatcga gggaagtttc ctgggcccgt tcgggaccgt ggacctgacc acggtccatg
    12241 actgcgtcat cggcagcttt gcctacgtcc agaggggaga actggcccat gcccgcatcg
    12301 acccggggcg gatctgggtg caggcgggag atgtttttga attcaactac cagtacccca
    12361 tggaggtcct ggagaagtac attcacctgg aagccggcaa gaagccccga ggaaagctcg
    12421 tggacttcgt ggaagggcgg aagaaggact acgaggagat cttcggcctg gtcggcaagg
    12481 agctcctaga ttccttgccg aagggaacca aggtgagccg ctatgccgtg gtgaagggaa
    12541 agaaccggat cgaggagaat gtcctggtgg cccaacgggc ctatatcgag gattcccggc
    12601 tgggcagagg ctccaacgcc caggagaact gctacatcgt ccagtcggaa ctcgagggaa
    12661 acgacgtgac ggcccacggc ggcaaggtca tctgcgcgac cctgggcaac aaggtcttcg
    12721 tcggcttcaa ctccttcctt cacgggactc cgtcctgcag actcacggtg ggagaaggaa
    12781 gcatcgtcat gccccatacc gtcatcgacc tggaacaacc cctggaggtc ccccccaaaa
    12841 ccctcgtgtg gggctacctc cggtctcccg aagacctggc ctcccagacc atccccctcg
    12901 agaagctggc tcaccacaaa ggggaacttc agctgggaag catgtatttt cggggaagcg
    12961 gccacgagtt tgtgcacggt tttcagcacc ggatcgaaca tattctcgaa gccaacggtg
    13021 ccttcttcga cgggaacgcc catccgggcc acgcccagaa gacccggaac atctcgtaca
    13081 acaccattca accttatccc gatgggcctc tcaaggggct ttaccccacc atcgagatcc
    13141 gcccgtaggg accggaagac cccgggactt gtcccggagt cttcctctcc cccggccctc
    13201 tcccaccggg ggagagcgaa ttgtctcccc ggccccgggc aagggtcgtt ttttcccttt
    13261 gcctcgatgg gagtgaccgc caaaaggatg tcgggacagg gcccttcaac acgatgtcgt
    13321 ctcgcacggc acccagcagg aggaagaaat gaagataggg ttggttttct tgttgctgat
    13381 tccgggcatg gtcttgggcc cggccgcagc ggctccggcc gaagaatccg ttacggtcta
    13441 ttcggtgccc caatcggagt cggccaaggc cgccgagtat tggactcccg agaggatgcg
    13501 aagggccaag ccgcgcaaaa tgcccgtgcg cccggaaaag cctgcttcca cccaagcctc
    13561 gggtgaggag gagatccggg gcgaacccgg gatggatccc ggcgggatgc ccggagaaaa
    13621 atctccgaaa cgccgctcca aacccgccat ggagccggag gtccaaaagg ccgatgccac
    13681 ggcggaaggc tacaagtacc cgccccccca aaacaccttt gcggttccca aggaatacta
    13741 caagacctat ccctacatgg ccgtggggaa ggtcttcttc gtcgaagacg gtgaggacaa
    13801 tgtctgctcg gcttcttcca tcgggggaaa cgccgtcatc accgcaggcc attgcgtctc
    13861 cgatggaaaa ggccatttcc tagagaagtg ggtttttgtc cctgcttaca tcaaggaagg
    13921 aaagaccccc gtcaaaccct acggaacctg gcccgccacc gacttcattc tcttcggcag
    13981 ctatcacaag gggggcgaca taggccgtga cgtggccttt gccgtcacga aaaacaacga
    14041 ccagggcgaa aagctctccg acgtggtggg ctacctgggc tttgcctaca acaagagccg
    14101 ggtgcagcac tggaacatgt tcggctatcc ggcggtggaa cccttcaacg ggcgcatcct
    14161 gtaccagtct caggcttcct acgccgacaa ggacggctcc gtggaaccgc ccaccatggg
    14221 aatgggaacc acccagaccg ggggctgcag cggagggccg tggattttgg agttcacgcc
    14281 tctaaacgac accgacggca acttcgtgtt cggggtcaac agctacgtat acggcaacca
    14341 gcccaagctg atgtatgccc cctatttcga cgacgaggtg aagcgactca aggaccgggc
    14401 ggtagccaag taagggacct tccttcccgg gaaatctccc cgctcccccg cccgttcccc
    14461 actcgttccg cttcgattcg ctcgaatccc gctttcaggc agtccttcac atcccttgta
    14521 aggcggtcct cggcgatgcg aaagagcacc cggttttctt tctccatgtg ctcccggagc
    14581 agccgcacgt aaccctccgc ctgtctctcg acctcgggga gaacggatcg gttcccccgg
    14641 tcgaggtcgg ccagggcact gcgcaaggcc cgcacgtagc cgcgtccggc attgtgctcc
    14701 gccagcatga ccccgatggg gccccgttcc gggagacccc ggcctcctcc aggaccggga
    14761 acagcagttc ttcttccttc ccgtggtggc aggcgtccac aaagatggtg aaaaattcga
    14821 tcaaacgccc cggatcttcc gccgtttccc ccagctccgt tcctttccag ccttggaacc
    14881 atccgggaaa aggatctcga gggagagtct gagcccttcg tgctcttgcc tcagttcctc
    14941 gatagggttc atggtccgcg ctcctttccg caatccttcc cgggccgtgg gggaagcccg
    15001 cagcccggga ttgccgggcg gagaatttcc gcccggaaga cgccgccttc gcgacttcaa
    15061 gttacctggc ttgccatgcg gccttccccg cggggagttc cgcgcctttt gctgcgcggg
    15121 cgtacgacca gccggtcttg agtcccgcaa agaaccaggc cggggaccca acccccacca
    15181 ggaagaccgt gtccccgatc atgcgcagcc accgcagagt tgccaaaagg ggctgctgga
    15241 ggtactcggc gctccgggcg taccagagcc gtgctcgatg cttgccgccg cctgcatgag
    15301 gccgatggaa agaaggctca gggcgatcat gagcaccagt ccgccgttca ttccccagaa
    15361 ggcgatctgg aggggccgat cccgccacac cccctcgggc tgcagccttc tcaccaccag
    15421 gagcaccagc cccagggccg ggaggccgta gaccccaaag agggccgcat ggccgtggac
    15481 cggggtcgtg ttgagcccct gcatgtagta gagggcaatg ggcaggttga tcaggaaccc
    15541 aaagatgccc gcccccacca ggttccagaa ggcaaccccc aggaaggacc ggacgggcca
    15601 ccggttcttt tccatccagg gcgcactccg tcccagtcgg taggtctcga aggcctcgaa
    15661 cccgatgagg gccaggggca ccacctcgag ggcgctgaac gtggctccca cggccatgat
    15721 ggaaatgggg gtcccggaaa aatagaggtg gtgaaacgtg cccggaattc ccgcgaccag
    15781 gtagagggcc gacgccatga ggaccgcgcg cacggcgccg gcggctcgga ccaggcccat
    15841 ttgagtgaag aggaaagcca tggtcgccgt ggcaaaaact tccatgaaac cttccaccca
    15901 aaggtgcacg acccaccatc gccagtattc cacgatggaa aggtgagtcc gcgccccgta
    15961 aaagaagccc gccccgtaga aaagcccgat ggccgcggag ggacccgcaa aaagcagcac
    16021 cagctgcctc cggtgatcct tgtgctcgag ggcgggccaa agccttctga gcatcaaggt
    16081 cagccacagg agaagcccca cgaaaagaag gatctgccgg aagcggccca ggtccacgta
    16141 ttcatacccc tgatgcccga accaggaccc ggtatccagg cccagcacct gttgaacgga
    16201 gacctattcc ccggcaaggg accccaggac cacaaaaacc agggcgccga acagggcatt
    16261 gacgcccagg acctggaacc gaggctcacg gccccccacg gccggggcca ggtaaagtcc
    16321 ggcggtaaga aacgccgtag cgatccagaa gactgccggc tggatgtgcc gggtgcgggt
    16381 cacggcgtag ggcagggttt gggacggagg gattccgaag aagccgcctc cttccaccgt
    16441 gtagtgggcg gtgagggccc ccggaagcgc ctggagcgca aacagggcga tcacgacccc
    16501 ggcgaacttg gccgtggcct tcatggaggc cgtggtctcg atgccgtcaa aaggatcggc
    16561 tgcgggaggc gggggtgctt catcctctcc ccggtgaaac agcttgaagg cgaccaggga
    16621 cccgattccg gcgatgagga gggccacgct caccagggac cagagcaggt ttgccgagct
    16681 gggggtgttt cccaccagcg gctcgtgggg ccagttattt gtgtaggtca cctccgcatg
    16741 gggacgctga gtcccggctg cccaggccgt ccagaagaaa aacgcggtca aggcccgtcg
    16801 cctctcgagg tcggggacgg tggattcctt gagggcatag gcccctcgaa gccgcctcag
    16861 ggccggtccc ccccgaagag cgcccggaag tgcccggcgg tttcggccat ggcccgcgca
    16921 cgttcccggg aagcctcgag ggtccccgag gagggatcgt acgtgttggt gcgaagggca
    16981 accttgactt gctcccccac tgctgctccc gcggcggaga gctcgccgaa ggacctgccg
    17041 tagagctcga ggttcgtgat ctccctcaat gccgtggcct cccggtggag ccagtccgcc
    17101 gaccagtcgg gggcctgata cgccccgtga ccccaaatgg agccgacctg gtggcctccg
    17161 atgctctgcc acccctgccg gccccggagg atgtcctccc gggtcaggag gatttctccc
    17221 gaatgggtcg ccacccgttc cgggatcggg ggaacgtttc gatagacttc gcggccgaac
    17281 caccccaata cgaaaaaggc cgcaaagatc acgagaccca cggtgatcca taatccttta
    17341 ttgtctctca tggccgacct cccgccctcg aaaggctctc tttccaaccg ttgtcctacg
    17401 ccgtttgctc actttcgttc attgacttag gcaaggggaa tgtcggcctt ttgagtatgt
    17461 gaaatccacg ggttgcaaag aacattgccg gaaggaccac aaagaggttg ttgtcttttc
    17521 tgtaaccgtg ttatacacct gacaaccatg gaaaccttcg acaccctcat caccgttgcc
    17581 ttggacctga cggcggcctt gagcgccggg gaccggtatc gaaggctcct cgaagctctt
    17641 gggggcgtga ttccttacga tgccgcttct ctgctgcgtc tggagggaga agacctggtg
    17701 cccgttgcgg ctcgagggct gtcggcggac gctttggggc gccgctaccc tttggaccga
    17761 catccccgcc tggcacgcat ctgcagggcc tcggaacccg tgcgcttttc cgccgacgat
    17821 ccgtcccccg atcctttcga cgggatgatc cggggggatc ctagcgcggc gcgccgcgtt
    17881 cacgcctgcc tgggatgtcc cctctacgtg gagggagagc tcatcggggc gctcaccgcc
    17941 gatgcgttgg accccacggc cttccaaggc ctggatcaga agtttctgcg agccatcgcg
    18001 gccatggccg ccgctcagat gcgggcggcg gacctcatgg aagccctcga gaacagcgcc
    18061 gtgcggcagg ggatgattgc ccgggatctc atggcggatt tgcaccgggg caaggagatc
    18121 ctgggatcga gccttgccgt ggagcacctg aggcgggaga tcggtttggt ggcgggatcg
    18181 gacttcaccg ttctcgtcct gggtgaaacg ggggtcggca aggaattggc ggttcgagcc
    18241 attcacgcgg cttctcttcg ccggaatgct cccttgctgt acctgaactg tgcggcgctt
    18301 cccgagggcc tggcggaaag cgagcttttt ggtcacacta agggggcctt tacgggggcc
    18361 gtccgggatc ggccggggaa attcgaggtg gcggacggcg ggaccctgtt cctggatgaa
    18421 atcggggaat tgcctctttc catccagccc aagctccttc gggcggtgca ggagggggag
    18481 gtgcggcgga tcgggtccgg ccggaccgtg cgggtcaatg ttcggctcct tgccgccacg
    18541 aaccgaaacc tcgaggaaga ggtccgggag gggaggtttc gagccgacct ctttcatcga
    18601 ctcagcgtct atcccctggt ggttccgcct ttgcgggagc ggcgcgagga catccccttg
    18661 ttggccgggc atttctgtga agcgatccag cgacgcctgg gcctggggcc ggtacggttg
    18721 gactcggatg ccctgggtct gctccggcgt tacccctggc cgggcaacgt gcgggaactc
    18781 gaaaacgtgc tttcgcgggc gatcctcaag gctggggccc aagtcccccg gggaaaattg
    18841 gtggtggtcg ctcccgtcca cctgggggga gatctggaag ccgccccggg aattggcgac
    18901 ccgtcacctc cccgggagac gctgcgggtt tcggccggcc gttcctttcg cgaagtggtg
    18961 gaagatttcc aaaaggacct cattcttcaa accgtggccg ggaaccgcgg caactgggcc
    19021 gcggcggccc gggagttggg catgcaccgc agcaacctgc acaagctggc ctcgaggctg
    19081 gggatcagga atcccggcgc caaaagataa tctcgggagg acggactttc cctcctggta
    19141 tggtttcccg tgacttcgag gggcaaggca agagttcgcc tcccgccgtg gccgggacag
    19201 ggggtagcgg attcccggcg gggtttcggg ggggagatcc ggagagctcc ggattttatg
    19261 gtattatttc acggtacagg gaaggaggat cccatgagcc gagttgccgg agaccaatcc
    19321 atttcccgcc gagatttcat gaagacggct gcggccttgg cggcaggaat gaccgcccgg
    19381 tgggatgtac tcgaggccct tgccgccggg atacccaagg gggacctgcc ggtggtggtc
    19441 atcggggccg ggctgggagg tctgagtgcc gcagcgctcc tggcccggaa cggttttccc
    19501 gtaacactgg tcgagcaaca cgaaaagccc ggcggctatg caacgacctt cgaccgtgcc
    19561 gggggcaagt tcaccttcga cgtgtctctt catctcgtca gcggagccaa gaactggctg
    19621 gggcccattt tcgaggggac gggcattcgg gacaagatcc aaatggtgca actgcccgag
    19681 ctgtgccgca tcgtggcccc tcaccatgac ttcctttggc ccaaccaccc cgatgccgcc
    19741 atcgatgcgc tgatcgagct ctttcctacc gaggcgctgg ggatccgggg gtttttcgcc
    19801 cagatgctgg gcctcgcgga tgaaatcatg gtgcccttct cccccgattc gatcctggac
    19861 agggtcttct ttcccctgac ccacaagcgc atgtgggccc ttcgaaacaa gaccctggcc
    19921 gaggtgttgg acgcccatgt tcgagaccct caactgaggg gactgctgtc cgcctactgg
    19981 ggctattacg ggctgccgcc ttcccggctt tccggcttcc tctacaccgt tgccacggga
    20041 tcgttcctgc gcttcggggc ccactacatc aagcggcgtt cccaggatct gagcgacgcc
    20101 cttctcgaag ccgtcgagac ggcgggcggc aaggtgctcc tcgagacgga agccgtggga
    20161 atcgagatga ggggcgggaa aattgccggg gttcgcctcg agggcggcac gctgctgccc
    20221 gccggggccg tcatatccaa cgccggcgtg ccctcgacgg tcaagatgct tccctcgggc
    20281 gccttgcccg gggattatct tcggaagctc gagggctatc ggccgtccct ttcgaccttc
    20341 tgcgtctggc tggggctcaa cggtgagatt cgagacaggg tcaagggcta cgaggtgttc
    20401 gtgctccggg actacgatcc cgagagggcc tttcgggcgt ccctggtgtg cgatcccgcc
    20461 gccgggtccc tggccgtgac cctctacgac aattgctacc cggggtattc ccggcccggc
    20521 acctccacgg tgtccatcct catgtcctgt gcctacgaac cctggcgccg gttcgaggcc
    20581 gactattttg ccggacgcaa ggatgcctac cggaaggaaa aggagcgcat cgcccgggtg
    20641 ctcgtcgagg aaacggagcg cagggtcatt cccgggctga catccatgat cgaggtcatg
    20701 gaagcctcca cccccctcac caatcttcgc tacaccagga accccgaggg ggccgtctac
    20761 ggctacgagc agaccctgag caactccttc atgacccgtc tggacaatcg aacccccctc
    20821 aaggggctct acttcgcgtc ggcatggacc tttcccggag gggggtacga accatgtctg
    20881 gaatcgggcg cccgggccta ccgggccctg gtgacggatt ggaccccacg ggcctgaaac
    20941 ggccccaccc gcatttctcc cggccgacag ccggggttgg aagccggtga ccctcagcct
    21001 cgaagccccg ggcaatcctc gccgtttccc cgaaaatgcc tttcctcgag ggggggagtg
    21061 gattatagaa aggcaaacgc cgggacggag atccggtttc cgggatggaa gcccggaggg
    21121 tcccgagaag agaggggagg ataggacaat gatcgagcga tacacccgcc cggaaatggg
    21181 gcgaatctgg accctggaaa acaagtatcg caggtggctg gatgtggaaa tcgccgtctg
    21241 cgaggtcctg gccgaggagg gacgggttcc cgcccaggac ctgaaggaga tcaagaaccg
    21301 cgcaggcttc gatgtcaaga ggatcgatga gatcgagcag acgacccatc atgacgtcat
    21361 cgcgttcctg accagcgtgg cggaacatgt gggacccgcg tcccggttca ttcaccaggg
    21421 gctcacctct tcggacgtcc tggataccgc caacgccctg ctcctgaccg aagcggcgga
    21481 cctcctcctc gcggacctgg atcggctcct ggaagtgctc aaggcaaagg ctttcgagtg
    21541 gaaggacctg gtcatgatcg gccgctccca tggaatccac gcggaaccca ccactttcgg
    21601 catcaagttc gccctgtggt ttgcggaaat gacgagaaat cgggaccggc tggcccatgc
    21661 ccgggaatcc gtgcgggtgg gaaagatctc cggtgccgtg gggaccttcg ccaacatcga
    21721 accccgggtc gaagagaagg tgtgcaagaa gctgggattg atgcccgccc ccgtgagcac
    21781 ccaggtgatc caacgggatc ggtatgcgga gtttttcgca accgtcgcca tcgtagggtg
    21841 cacggtggaa aaaattgcct tggagatccg gcatttgcag cgaaccgagg tgcgggaagc
    21901 cgaggaattc ttccgggaag gccaaaaggg gtcttcggcc atgccccaca agaggaatcc
    21961 catcgcttcg gaaaacctgt ccggcctcgc ccgggtcctc cggggaaacg ctcttgcggc
    22021 catggaaaac gtcgctctct ggcatgagcg ggacatcagc cattcctcgg tggaacggat
    22081 catttgcccg gattccacca ttctcctgga ttacatgctc aaccggttga cccgggtcct
    22141 ggaaaacctg gtggtgtatc ccgaaaacat gcgcaaaaac ctcgatctca ccggcgggct
    22201 cttctattcc cagcaggtga tgcttgccct gacgggaaga gggatttccc gggaagaagc
    22261 ctatcgactg gtccagcgca acgccatgaa ggtttggaac cagggaggaa ccctccaaga
    22321 acgcctcagg gaagacgcgg aggtcatggc ccttcttccg gccggaatca tggatggcct
    22381 attcgacctg aagcatcacc tccagcacgt ggataccatt tatcagaggg ttttcggctc
    22441 tcatgaatcc tcttcctgaa ttgctggcac ccgccggggg cgttgaaggc ttggttgccg
    22501 ccgtcgaaaa cggggccgac gccgtctacc tggggttgaa agaatacagt gcccgggctt
    22561 ccgcagccaa tttcaccgta gaggaactgg ccgtgctgct tccctacgcc catgggaagc
    22621 aggtccgcat ctacgtggct ctcaacagcc tgttggggtc ttcggatctc cccaaggttc
    22681 tggatcagct ccaggccctc tccgacctca agcccgacgg gatcatcgtc caggacccgg
    22741 ccgtggttca ttttgtccgc aggtactttc cggatctgaa gcttcacgcc agcaccctca
    22801 tgacgattca caacagtgcc ggggcaaatc agctgcagcg catggggttc caacgggtgg
    22861 tgctggctcg tgaactgacc ctcgaagaga tcgaggggat cacggccaac acgtccgtag
    22921 agctggaaat cttcgtgcac ggagccctct gctattccta ctcggggctc tgcctggcca
    22981 gcagctttcg aggggggcgc agcggcctcg aggggcgctg cgttcaacct tgccgcctcc
    23041 ggtttcgcca ggggcgcaag gaagggtttt ttctttcgtg caacgacctg tgtgccctgc
    23101 ctctcttgtc ccgcatcaag ggcatgaagc tttccgccct gaagatcgag ggcagactca
    23161 agtccgccga ctacatcggc cgggtggttc gggcttatcg aatcgtcctg gacgcctccc
    23221 cggaacggga gaaggaggcc ttgggggaag ctcgggccat gctgtccctg gccccgtctc
    23281 gacgcctgac cacggggttt ctcgaggatt cgcgggggcg ggacgtcctg acaccccatc
    23341 gtgggggatc gagcggtttg tgggtgggca cggtttcctc cgttactgcc ggtgccgtca
    23401 ccgtgctcct gaggcatgag atccgaacgg gggaccgcct tcggccggac tcgagccggg
    23461 gcgaggagaa ggaagctttc acggtgatcc gcatgcgggg ggagcgggga gaagaactcg
    23521 tccggggcga ggcgggagaa cgggtgcacc tcgaggtgca ggggcatttt gcacccgggg
    23581 agcggctttt tcgcatcggt tcccagggtc cccctccggc atccctgtgg caacggattc
    23641 gatcggaagg ccgggaatcg gcgcggttcc gacgaaaatt tcctcgttcc cgggatgccc
    23701 tcgaagagct cgatcagggg caaggggagg aacaacgagg aacaacccgt gaggtcgtct
    23761 ttctcaagat cgcctccctc cgggacttgg gtgccgcctt ccagatagag aacgtacggg
    23821 tggttttgcc cgcaacccgg tccaatctcg aacagctggc caaaaggaag ctttcagcgc
    23881 aacagaagcg ccggtttgtc tggtcgctcc cgcccctcct gtgggagtcc gacctggagt
    23941 atacccgggc ggccgtggcc tggtaccggg caagagacta ccagacctgg gaatggaaca
    24001 attggggaca cctggatctc tttgcggggc gggaacacgt ccggcttgtg gccgggagcc
    24061 gcctgaacgt gaagaacggg gccgccctgg ccgccctgct ggaaaacggg tgcggcgcct
    24121 gtgtcctctc tccggagatc actcgggaag agttgttgca cctgggtgct catggccccc
    24181 gggttcgaat gatcgtcccc gtctattcgt ggccccccct gttttcctcc cggttgctcc
    24241 ccaagctgga cacggaacga cctcttttga ccccccgggc ggagcccctt ttctttcgaa
    24301 cggaccctcg gggaacctcg tttctttacg cggaccgacc ggtgaactgg cttgcccagg
    24361 tccccttcct tcgggcaaac gggttttccc gcttcctggt ggatctcagc gacggtccga
    24421 accccgaggc ccataccctc acccgggtct gggaagactt ccgaagggcg agggctacgg
    24481 gacctcacag cctcttcaac tatgaccggc agacctcccg gggaaaaagg ggacctcgga
    24541 gaaaagggtc ggaaagaaac cgatgatcga gtggttgagg gtatacggca atcgcagggt
    24601 cctgatgctg ttgggcctgg ggttttcctc ggggctgccc ctggccctga cgggctcgac
    24661 cctccaggcc tggatgaccg tcgaaaaggt ggacctgcgc gtcatcggca tcttttccct
    24721 ggtggggctc ccgtacaccc tcaaggtcct atgggctccc gtcatggatc gattcaccct
    24781 tcctttcctg gggagaagac gcgggtggat cggcctggcc caggtcttgc tggccctggg
    24841 cctcgtgggc ctgagttttt cctccccggc ctcgatgccg tggctggtgg cgttcctggc
    24901 gttgaccgtg gcctttctca gcgccagcca ggacatcgtc atcgacgcct atcgcgccga
    24961 cgtgttgcgg gaaggagaac tgggcgcggg agcggccacg acggtggtcg ggtatcggct
    25021 ggccctcctg gtatcgggag ccctggccct cattttgtcg gatcaccttt cctggagtca
    25081 cgtgtacatt ctcatggcgg tcctcatggg ggggggcctg tttttcaccc tgggagctcc
    25141 cgagcccgaa gagaaggtcg ttgccccccg gagtctccgg gaagcggtgt gggagccgct
    25201 ggttacctat tttcaccgct cccgggcggt ggaaatgctc cttttcatca tgctctacaa
    25261 actgggggac gccatcgccg gagccatgac caccccgttt ctcctggaca tgggattcag
    25321 ccgcacggac gtgggaatgg ccaacaaggc cctggggctc atcaccacca ttgtgggcac
    25381 gttggcgggc ggggggatca tctcgaagtt gggaatccac cggtccttgt ggattttcgc
    25441 ctttcttcag gccgggtcca atctcacctt cacggccctg gccgtggccg ggaagaacta
    25501 tcccatcatg gtgggggccg tcgccgtcga gaacctctgc ggcggcatgg ggacggcggc
    25561 tttcatcgcc ttcatgatga gtctttgcga caagcggttc acggccactc aatatgccct
    25621 gctcaccagc ttcatggcgg tgacccgcgt ggtggcgggg gtccccacgg ggttcatggt
    25681 gaactccctg gggtggccga tgttctatgc cgtgagcgtg ctgggagcgc ttccgggaat
    25741 tcttctcctg ccccggtttg ccccctggcg ctccgttccc gaaagaaaag agccggcctt
    25801 acaggggacc cggtgagagc ccttcttcgc tcagcgcagc aggtgggtgt ttccgatgcg
    25861 aatccgcctg agacctgcgc tgcgggccgc ctccaggcag gattccgcgt gatcccggga
    25921 tgtggtggga aaatcgttca ttcgaaactg gggggcgaaa gccaggaggg tgtatgggat
    25981 gtccgggtcc agttccgcta tgaaagaggc gatccctctc acttcgtcct cccccacgta
    26041 gccgggtacc agcagagtgc tggcgacgag gaagggggg
//
LOCUS       DAOOVC010000046        25952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1264_length_25952_cov_6.828899, whole genome shotgun sequence.
ACCESSION   DAOOVC010000046 DAOOVC010000000
VERSION     DAOOVC010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 25952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25952
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_25952_cov_6.828899"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLM79_08055"
     CDS             complement(<1..492)
                     /locus_tag="PLM79_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86301.1"
                     /translation="MNSRIKILIVDDDDVCRELLRDAIEDDEVDATLASDGMDALEKL
                     RTSSFDILITDLNMPRMDGLTLLTQARQIHPHILTIIITGYGSLESAIEAIRLGAYDY
                     VQKPFKIERITIMTRNAVEKVKILREKNRLLKDLEFVYQKLRLLEGECHAPADRAPAP
                     QSDL"
     gene            complement(534..932)
                     /locus_tag="PLM79_08060"
     CDS             complement(534..932)
                     /locus_tag="PLM79_08060"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOV86302.1"
                     /translation="MEAKIQEITPTGAEQWLRQDAALKEASLIPPVTKAEKRGEAKVS
                     TQDKQGRASESPGMEKGKAKELVEEVQSYLEDLNIQLSFDLSEKTGEVVVRVIDRDTG
                     EVIRQIPPENLLDLREKLKELRGVLLDGTV"
     gene            complement(1042..1710)
                     /locus_tag="PLM79_08065"
     CDS             complement(1042..1710)
                     /locus_tag="PLM79_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86303.1"
                     /translation="MRSSFSFRPRKDSAVIGLTARRILALLCVGLGLSGCMGALQATS
                     PRPVHCEPPQVLMQTDFARYRQSRLGILLFEVPPYASEAAPEVTDAYYRELLSTGLFR
                     TVKILPYTVKNDQEALWWARSEGWDLLMRSGIQYLLDGSGALPTHIEVRTRILDVRTG
                     QPIWEVLQKAYSEPGADVDLFWTTVPGKPAQRYRLLSSALGVQLAQMLIPPEEKTTRP
                     MRSK"
     gene            1987..3069
                     /gene="rfbB"
                     /locus_tag="PLM79_08070"
     CDS             1987..3069
                     /gene="rfbB"
                     /locus_tag="PLM79_08070"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOV86304.1"
                     /translation="MVGTHKRTFLVTGGSGFIGSSFVLQSVMSGHARCVNLDKLTYAG
                     NPLNLEALADHPDYTFFRGDLGDRDLTNGIFRDFQPDAVVNFAAESHVDRSIHDPGVF
                     IGTNVVGTWNLLDETLRYWERLDGERREGFRFLHVSTDEVYGSLGPQDPPFHEGTPYD
                     PSSPYSASKAAADHLVRACHRTYGLPVLITNCSNNYGPRQFPEKLIPLTILNAFQGKP
                     LPLYGDGLNVRDWLYVEDHCAALRTVLERGRVGHTYNIGGRCERTNLDTVTTLCRLMD
                     ELFPESPHRPHESLMAFVKDRPGHDRRYAVDCSKIREELGWTPSESFESGLRKTILWY
                     RANGPWVKSVQSGAYRRWIERHYGFH"
     gene            3130..4008
                     /gene="rfbA"
                     /locus_tag="PLM79_08075"
     CDS             3130..4008
                     /gene="rfbA"
                     /locus_tag="PLM79_08075"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOV86305.1"
                     /translation="MPSRWKGIILAGGAGTRLHPITKAVSKQLLPIYDKPMIYYPLSV
                     LMLAGIRDILVISTPEDLPRFQILLEDGDQWGVRFSYARQPQPGGLAQAFLIGRDFIG
                     KDPVCLILGDNLFYGFGFRELLGKAVRMERGGLIFAYRVVDPQRYGVIEFDEEGRAVS
                     IEEKPQRPRSRYAVPGLYFYDNQVVDIAAGLKPSARGELEITDINREYLRRGCLQVQV
                     LGRGFAWLDTGTHESLLEASCFIETIEKRQGLKVACVEEVAYRMGYIGAGQLRRLAEP
                     LMKNGYGRYLLRILEE"
     gene            4195..5109
                     /gene="rfbD"
                     /locus_tag="PLM79_08080"
     CDS             4195..5109
                     /gene="rfbD"
                     /locus_tag="PLM79_08080"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOV86306.1"
                     /translation="MEPSADLHPRVLVLGSGGMLGRDLLRRFRDSGIPVLGLGSGECD
                     ITRRDRVAACVESRPWGAVINCAAYTAVDRAEAEPGAAFRVNRDGAAHVAEACRRENV
                     PLIHLSTDYVFDGQSRVPYREEDPARPVSVYGRSKWEGEEAVRDRSGKHLVVRTAWLY
                     AAHGQNFVTSVLHLARRRKELRVVDDQFGCPTWTEDLAEGLVSMVRKVLESPKGVPWG
                     TYHLCGGGFTSRHAFARAIVEEGYRREHLVVGRILPVPTDHYPTAARRPPWAVLDCQK
                     ANNAFGIALPPWQTGLARMMEELYTPTA"
     gene            5196..5654
                     /locus_tag="PLM79_08085"
     CDS             5196..5654
                     /locus_tag="PLM79_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOV86307.1"
                     /translation="MIEGVVVKELKTHLDDRGFFREIIRVTDDFFGEGFAQWSHSLMF
                     QGVIKAWHVHRRQVDWWYVAVGVLKVALYDLRPDSPTHKRLMECRMGEGQTADVLRIP
                     PGVAHGCKVLQGPSHLFYITSKVYDPEDEGRISHDDPGIGYDWTSPPGIR"
     gene            6314..7333
                     /gene="pseB"
                     /locus_tag="PLM79_08090"
     CDS             6314..7333
                     /gene="pseB"
                     /locus_tag="PLM79_08090"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HOV86308.1"
                     /translation="MLNDKVILVTGGTGSFGQAFTRAVLSRYKPRKLIIFSRDELKQS
                     EMRERFPRDQFESLRFFIGDVRDRDRLYRSFDGVDIVVHAAALKQVPTAEYNPIEAVK
                     TNVLGAANVIDAAIDHNVQKVIALSTDKAVSPVNLYGATKLCSDKLFSAANSYSGYHS
                     TRFSIVRYGNVIGSRGSVVPLFLKLRTSGVLPVTDPRMTRFWLTLEQGVDFVLASLQR
                     MKGGEVFVPKIPSMKVMDLAEAIAPDCRVEITGIRPGEKLHEFMITPDDARHTLEYES
                     YYVILPTYRAGELHHYMDHNGGKCCPEGFRYSSDGNSWWLSREEMQRVVEHLCGTKPG
                     MGHGA"
     gene            7330..8514
                     /gene="pseC"
                     /locus_tag="PLM79_08095"
     CDS             7330..8514
                     /gene="pseC"
                     /locus_tag="PLM79_08095"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HOV86309.1"
                     /translation="MKAVGDLPFLPYGRQTVDEDDIREVIEVLKSDWLTTGPKVEEFE
                     ATFAESVGAECAVAVNSGTAALHCAMYALGVGPGDEVVVPPMTFAATANCVVFQGATP
                     VFADVTPGTLLLDPARVDACINPRTKAILCVDYAGQPCDYDVLGEIARSHGIPLVADA
                     CHSLGGGYKGRPVGTLADISVFSFHPVKHITTGEGGMLTTNHPSLATRARNFRNHGIS
                     GDARRRSENGSWYYEMTDLGFNYRLTDFQCALGISQLSKLRGFVLQRQVLAGHYHRGF
                     SLIPEITPLLVRPEVSHAYHLYVVALDQDRLSADRNKIFAALRAQGIGVNVHYIPVHL
                     HPYYRQRFGTGPGMCPVAETSYERILSLPIFPAMSPGDVETVVDRVGDLVDRYRHPSA
                     QQ"
     gene            8532..9572
                     /locus_tag="PLM79_08100"
     CDS             8532..9572
                     /locus_tag="PLM79_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOV86310.1"
                     /translation="MRKVKIGPRQIGPEEPVFVVAEAGTTSNGDLPTALKLVDAAAEA
                     GADAIKFMILGPDYFMSDRTVTYEYEWAGGRHVENMYEMFKKLMFTEEEWFKIRDHCR
                     ERGIIFYATVDYLQGVDLAERLDVAAYKLSSWDITYLPLIEKVGSTGKPVQLDLGPAT
                     LADLAKAMQVLDQCGNDQVILVYCTHAAIDREVNLPSVPYLQQVFRCPVGYSADSLET
                     APDIAAVALGAHLIEKRLSLSRSYFGHHHIHAVEPDEFKEYLACIRRVEEMLGHPQLT
                     PSREDTRLKEHYYVSLVADRDIPAGTVITRDMIACKWPGTGIAPEFADLVVGRTARRE
                     IACNQVLGWDDV"
     gene            9586..10200
                     /locus_tag="PLM79_08105"
     CDS             9586..10200
                     /locus_tag="PLM79_08105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV86311.1"
                     /translation="MRKQAEAKRLEVLWTGDFGREYTRRNSQVTPSTGTFHHALGRQL
                     EARRILEVGCNIGLNLTRLAADGHFAVTGIDLNGYALQEARRRLPHANLLRATAYRLP
                     FADESFDLVFTCGVLIHIPPESLDDAIREIHRVSRRWIWCGEYFSEHLTEVPYRGEKG
                     ALYKCDFGASYLDLLPDLELVRRGTLAKESTGFDNLETWLFKKG"
     gene            10207..11265
                     /gene="pseG"
                     /locus_tag="PLM79_08110"
     CDS             10207..11265
                     /gene="pseG"
                     /locus_tag="PLM79_08110"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HOV86312.1"
                     /translation="MSRILFRCDGSSSIGMGHVVRCLSLARSLRQAGAQVFFLLRELG
                     GGAQGKVRELGFDVLTLPGPDFINGALPEWDRLRTAEAAGEKGAGWVLVDHYAAREDY
                     LEGLRSLGLRIAVLDDMADRTLTACSWLLNQNLGADALPYRTTVECVRLLGPEFALLR
                     PEFHAARQALSREFSRRDAHVILTLGGGGTGELSIEVLRALDGVSSPLEIRCIQGGSD
                     PVPAELRHAAGSSMHKVSILRDVRNMAAHMLWADLSVNAGGSTCWELCCLGVPMITLV
                     TSEDQAMIARALEEHGCARNLGEWTPGRTAARLAETVRELIDNPDVRLEMSRKARRRV
                     DGLGAKRAADSLLDLMNK"
     gene            11285..12331
                     /locus_tag="PLM79_08115"
     CDS             11285..12331
                     /locus_tag="PLM79_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOV86313.1"
                     /translation="MKGLRIGTHLIGERAPCFLVAEIGINHNGSPDLARKMIDAAADA
                     GADGVKFQNYRTEDFILDRSLTYRYPSRGGWVTESQYEMFKRCELDRRALQELKEHCD
                     RRGVVFHSTPTSPAGVRDLVEIGTKVLKNGSDFLTHLDLIEHMGRSGVPTVLSTGMAT
                     LAEIEEAVRVFRKTGNDGLILLHCVSSYPTPPEDTHLRKIPALAAAFDCLVGFSDHTS
                     GTAAAIGAVVLGACWLEKHFTLDRSLPGPDHAFSSDPEEFGCLVRAVRDVEKCLGTSR
                     LALTTKELESRRDFRLSCVAAGDLACGHRIREEDVVFRRPGTGLPPSCLPLLVGRILR
                     KDVAIGEVIRWADI"
     gene            12422..13132
                     /locus_tag="PLM79_08120"
     CDS             12422..13132
                     /locus_tag="PLM79_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV86314.1"
                     /translation="MPQEFWIEEYRKKAASADPILQSGRGKQFDAVELLYVVKQVVEL
                     LRLDASHELLDVGCGNGLLDIVLSACCRHVMAIEPVEELAELARKNLRHCPNVAVEVG
                     HGADIRAANDTFDCMLAFGVLQLVSPEEAIEMFGEAVRVVRPGGRVVFGSILDAARRD
                     RFLIPYLDGVRGGSHLSADEKEEIIRRNLSAHWHHPKDLADWWMGRGCSVTIHSLVAG
                     DPDADHRFHLVVTVEKHA"
     gene            13129..14031
                     /locus_tag="PLM79_08125"
     CDS             13129..14031
                     /locus_tag="PLM79_08125"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOV86315.1"
                     /translation="MRIVFLGNHTVGVRALEALLQKDEVVGVVAHPPDPEDGIRYLSV
                     YDFSRRRGLHAVRKNGRDPGLEEFVRDCRPDLLWITDYRYLLPQGVLTVAPLGVVNLH
                     PSLLPAYRGRAPINWAILHGERELGLTVHFVDEGMDTGDIIEQVPFFLSENQDVGDAL
                     CILYPLYGEITRRVIAHFRKGKVPRRPQDHRRATVFPRRTPEDGLIQWEQRAERIRDL
                     VRAVAPPYPGAFSFCHGRKIRILSAAVRSEEGVHGEPGSILSLDSTGPVVCAGTGTVW
                     IRKFEWEPADPPGRLNPGDRMENR"
     gene            14028..14645
                     /locus_tag="PLM79_08130"
     CDS             14028..14645
                     /locus_tag="PLM79_08130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV86316.1"
                     /translation="MKSPQEQAEDRNIVNYYTELLEKHGRNPRSLDWGSRRTQELRFR
                     VLCDIAPLDGCSLLDVGCGLGDLWDYLSSKGIGVFYTGYDFTPAMVAEAKGRFPSVPF
                     EVRDILEEPDEHCRFDYVMASGIFYLRRDRPMQYLVETVRRMFGLCRIGVAFNSLSAR
                     ASRRDPHEFHADPSRVLSSCLEITPRVVLRHDYLPNDFTVYLFRD"
     gene            14654..15355
                     /locus_tag="PLM79_08135"
     CDS             14654..15355
                     /locus_tag="PLM79_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOV86317.1"
                     /translation="MIVTISQPRYLPWLGYFHRIAVSDLFIYLDTVQYTPRDWENRNR
                     IKTSRGPTWLTVPVKAGYRAMIPEVTIDNDQPWQAKHWKTLSMNYSRAPYFREYEQAL
                     GEIYQQKTWPELTALNLRSTRILCEQLGLDKARFARASEIGAPGKGSELILNLCTAVG
                     AKVYLSGSQGRNYLDEEKFRQAGIRVVYQDYVHPQYPQLFGGFAPFMAVPDLLFNCGP
                     GSLDILVTGQKEVLR"
     gene            16061..16741
                     /locus_tag="PLM79_08140"
     CDS             16061..16741
                     /locus_tag="PLM79_08140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOV86318.1"
                     /translation="MNVLVVAAHPDDEVLGCGGTIARLSREGHAIDVAILGEGITSRY
                     RARDDADAATVEALHERSREVARLLGVRDLSLHNLPDNRFDTVPLLDIVKIVEDLVSR
                     FRPEVILTHHGDDLNVDHAIAFRAVLTATRPVIAMPVKEIHAFEVPSSTEWAFGRLHR
                     GFRPNVFVNITTTTAAKIQAMRVYESEVRAFPHPRSPEALQALARYRGSSSGCEAAEA
                     FELIRWVR"
     gene            17233..18894
                     /locus_tag="PLM79_08145"
     CDS             17233..18894
                     /locus_tag="PLM79_08145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86319.1"
                     /translation="MSNRKRVLLVSYYGKVCLSTKILSGILRRAGHETCLIYLKDDRS
                     TQVESVNKASSCFQFICSDRGAAVYGTGMDVNPIRSTELDLLVNKALEFLPDVVAISS
                     RSVHIEVSRVIIMLLRKVLPKATRYIAGGYGPSLEPEKFLEVFDYVCIGRGDSAVLDL
                     VEKEDPSSCRNVATLVDGKLHIAFLDNQVDLNLRPFADWNFENKFLIEDGKIFPLHEK
                     YDPKTYGIIASEGCPSSCTYCQASQWPHIYRLHGGRSPKVVMRKPDNVIAELIHAKDH
                     FGIESVQFMDSIFTWHRKWFDEFMALYNRHVALPFCCYTDARFTSRKQLVRLKESGMF
                     STTVGIQSVDEESRRKVMGRRVSNERIIEFAWEVVELGIVPTYDIIVWNPFETEESLA
                     RGVDLLRQLPKSNRIFIYELKILPRSLLEDLFNECRPTPLSQESYMYWSWIFVMLLRS
                     DATQEAVPAILSNPIYKDSPHLIRERYLEILGSQEVRERLFAIRPVAMGEMLRPTMFI
                     EKASLNLEGVLGEDRHYITGKTIRRPLRTGEVLCYKDFYTSYQEK"
     gene            20389..>25952
                     /locus_tag="PLM79_08150"
     CDS             20389..>25952
                     /locus_tag="PLM79_08150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV86320.1"
                     /translation="MLSSLIHSDKGRNALEIQRQAYRFAYGLFYRINIPFPLVTMPNP
                     HKGILAYKSLEDLLPGREPNLDRIAGIILDRQPVCPEPTSEELQRSPEEEDAWFEKRD
                     NRVSVRMTPRVGGPLVSVVIPCFNYGPYLTEAVESVIGQSFQDFEIIIVNDGSTDNSR
                     QVAEDLIANHPGHLIRLIDQHNSGQPAVSRNNGIAEARGTYILPVDADDKLATSSSLK
                     ILLDEAIKHRDTPVAVVGWVQKFGVEDDLWKPKGFDPNRILRRCTVPYCALYHRSVWE
                     RQGGYRTNVPGYEDWDFWIGATIQGVRFVHLEAPVLLYRRTQFNSLVDKARMRHEWLM
                     AGIITNHSSVYEASEVAWAEDYRRRHPQPPKERRFSEEIQRNPEVAAALVASYPEHYA
                     EDEVKWAIQHLHRHPFLIHKGLPVAPNSAGKKILMLRGDVVSTPTQLALAWFRESLGA
                     LQERNFPEAERLMGLYRQSIRYETFTARDGRPEQLPGISVIIVAYRTKQLLLECLNSV
                     FEQSYRDFEVIVVDNGGNEEVHETLRSYPILHVQCPDNLILSEGRNIGVHHARGRIVA
                     FLDDDARVPRNYLESIDLAFKAYDIVGMRGKVHPKSTLSHHSHAGHYDLGDTPIPSTI
                     DTEGNSAFSRKAYQEVGGMNPLLFGMEGLDLSYRLAGRYGPHRTVYWPETLIYHDFAV
                     TEEKVEKKLAQHALMREYLLYKFPDIYDFHNRLRGHAANESSKLHGKALIPAKTCSAP
                     VSIETEATVTLAVVTYNRAKTIGRALESALNQSRPADEILVVDDGSEDKTAEVVAAFR
                     SPRIRYIRKEHSGAPQTRNRAVAEASGDFVLWLDSDDCLPPHTVASHLAALRRTPDAD
                     VLYGNLLIVDEDLKPQKEWIYPDYYGCNNRLLARMVRGNCLPNPGTMIRKSCFCRFGG
                     YDESFRRAHDYEFWSRLAKDAVFKHSGAVVCNWRVHANSLSAHGQGPETSYEARVVQG
                     MLKRYPLEDLYPDIMSAMEDRDEARATCYWQAASTFLEFKDIENVVTSLQKSYEYCPS
                     EEVQALLSYITKGKSSHPSGLPGSVIAAFNRLKHPPVPDPEDFDEAYYLESYPDIAQA
                     VAKGVFRSGWEHFARFGRFSNRLLARTTWNLSFHGSSGASPPQVSVVIPCYNHAHYLR
                     EAVQSVLRQTYRDWEILIVNDGSTDDTGRVARSLIQEHGSRRIHLIEKENGGLAEARN
                     VGVRKSRGKYILPLDADDLLEPTMIEECVRILDQNPGVAIAYTDVRQFGAVERVVPSV
                     PYDFKLLCHMNFMCCSSLYRREAFDAVGGYNPNMVRGYEDWDFWIGCGEKGFFGEHVP
                     KALFLYRRNANSMVTESQKYDRELKARIVLNHPAVFSPEQLSWAKGVEAKDPRIMALP
                     DQPHFIPLFESTRRSPSAGEATSGRETGMKVLFTMYGWNDTGGGTTFPKSVARELVQR
                     GHRVSVFYASLKNDPTMPPYSVLKEEEDGVVLYGVYNRPAPFVDPDCPEREIRDEGVL
                     ERFREVLDEVGPEVVHFHNFHGLTFALAREVRERGIPSCYTPHNYHMIDPHLYLFRSD
                     LSLWRNTDILENSEAVQRNPGKRELYKKRAETTRKLLNEWVDMTLAVSSRQRELLVQH
                     GARPDRVAVVHQSHASTDVLWSSPELAEQGSRPLHHPLRIGFIGGVMPHKGVHVLAAA
                     AQHFDSSRAEFHVYGFGSIEYIRFVRRMDPGERLVFHGNYTHDMLESIARELDVAVVP
                     SLWEDCAPLVLLELMAMRLPVLGASIGGIPDFVKEGVNGFLYSHNSVESLCRCIEYCI
                     QNRPIIRKMRSSLRRPHSFQGYVNHLETVYRDLSSRKIGDASRYDLLVRLGSVSSRPA
                     IRWEGSQFVIHSL"
BASE COUNT         5410 a         7252 c         7697 g         5593 t
ORIGIN      
        1 aagatcgctt tgcggcgccg gggcccgatc cgcaggagca tggcactcgc cttcgaggag
       61 tcggagcttc tggtagacaa actcgagatc cttcaggagc cggttctttt ccctaaggat
      121 cttgaccttc tccaccgcgt tgcgcgtcat gatcgtgatc cgctcaatct tgaacggctt
      181 ttggacatag tcgtaagcgc ccaggcgaat ggcctcgatg gcggattcca ggctgccgta
      241 ccccgtgatg atgatggtaa gaatgtgcgg atgaatttga cgggcttggg tgagcaaggt
      301 cagcccatcc atgcgcggca tattcaaatc ggtgatgagg atgtcgaagg aagaggttcg
      361 gagcttctcc agggcgtcca ttccgtccga agccaaagtg gcgtcgacct cgtcgtcttc
      421 aatggcatca cgcaggagct cacgacagac atcatcgtca tcgacaatca ggattttgat
      481 cctcgagttc aaaccacatc ctcgcagcgg ggtgtcgggc atcgccaact cactcatact
      541 gtaccatcta acaagacccc ccgcaattct ttcagctttt ctctgaggtc cagcaggttc
      601 tcgggaggga tctggcggat cacctcgccc gtatcccgat caatcacccg caccaccact
      661 tctccggtct tctcactcag atcgaagctg agctgaatgt tcagatcctc cagataggac
      721 tgcacttcct ccaccagttc cttggccttg cctttttcca tgcccgggga ctccgaggcc
      781 cggccctgct tgtcctgggt ggagaccttc gcctctcctc gtttctccgc cttggttacg
      841 ggcggaatca gggaggcttc cttgagagcg gcatcctgtc gcaaccactg ttccgccccc
      901 gtcggcgtga tttcctgtat cttggcttcc atggggatcc ccctcgatcc aaagagatag
      961 tgctccctta tcatttctcc tacactttca tcggcacctc gggggagaac ctttagagca
     1021 aagtgggtgt tgccatccga tctacttgga acgcatgggg cgggtcgtct tctcttccgg
     1081 ggggatcagc atttgggcca gttgcacccc cagggcggaa gaaaggaggc gataacgttg
     1141 ggcgggtttt cccggtacgg tggtccaaaa gagatccaca tcggcacccg gttccgaata
     1201 ggccttctgg agaacttccc agatgggctg gcccgttcgc acatccagga tgcgggtgcg
     1261 gacctcgata tgggtgggca gggcccccga gccgtccaga agatactgaa tgcccgagcg
     1321 catgagcagg tcccaacctt cggatcgggc ccaccagagg gcttcctgat cgtttttcac
     1381 cgtgtagggc agaattttga cggttcgaaa caaaccggtg ctcagcagct cccggtaata
     1441 ggcgtccgtc acttccgggg ccgcctccga cgcgtacggg gggacctcga agagaaggat
     1501 ccccagcctg gattgacggt agcgggcaaa atccgtctgc atcagcacct ggggcggctc
     1561 acagtggacc ggtcggggag acgtggcctg gagagcaccc atacagcccg acaacccgag
     1621 gccgacgcaa aggagcgcca aaatccgtcg agccgtcaaa ccgatgacgg cgctgtcttt
     1681 cctgggacga aatgagaaag aagacctcat accgccaatt ccgtatgtgg gatgaatgcc
     1741 aaggggtgga cgacgggaac cttccttccc ggaaagagtc aggaccccca ccccggaaag
     1801 gattcccgga cgaaacctcc ttatgcattt tgcgagccgg cacccaaagg gggcaattcg
     1861 ggaccgaacc ggaaaacttg attgaaacgg cctcggggat tatgctctac tccactcgga
     1921 aagaagcagg aaacggtctc ccagattacg tccaaccgtt tggcagcggg gggttcgggg
     1981 taaagcatgg tgggaacgca caaacgcacc tttctcgtta ccggaggatc cggttttatc
     2041 ggaagcagtt ttgttctcca gtccgtcatg agcggtcatg cacgttgcgt caacctggac
     2101 aaactgacct atgcgggcaa tccgctgaac ctggaggcgt tggccgatca tcccgactac
     2161 accttttttc gcggggatct cggggaccgg gacctgacca acggaatttt tcgagacttt
     2221 caacccgatg ccgttgtgaa ctttgcggcg gaaagtcatg tggatcgcag catccatgat
     2281 cccggcgtat tcatcgggac gaacgtggtg gggacctgga accttctcga tgaaacgctt
     2341 cgttactggg agcgactgga cggggaacgc cgggaaggct tccggtttct tcacgtttcc
     2401 acggacgagg tctatgggtc tctgggtccc caggaccctc ccttccatga agggactccc
     2461 tatgatccca gcagccccta ttccgcttcc aaggcggccg ccgatcacct ggtccgggcc
     2521 tgccatcgca cctacggact gcccgtcctc atcaccaact gctccaacaa ctacgggccc
     2581 cgccaattcc ccgagaagct cattcccctg acgatcctga acgccttcca gggcaaaccc
     2641 ctgcccctct acggggacgg cctcaacgtt cgggactggc tctacgtgga agatcattgc
     2701 gccgccctgc gaacggtgct cgaacgagga cgggtcggac atacctacaa catcggggga
     2761 agatgcgaac ggaccaacct ggacaccgtc accacccttt gccgactgat ggacgagctg
     2821 ttcccggagt caccccatcg tccgcacgag tccctcatgg cttttgtcaa ggatcgcccg
     2881 ggccatgatc ggcgatacgc cgtggattgc tccaagatcc gggaagaact gggatggacg
     2941 ccgtcagaat cctttgaaag cggtttgaga aagaccatcc tgtggtaccg ggccaacggc
     3001 ccctgggtga aatccgttca aagcggggcc tatcgacggt ggatcgaacg ccactacggt
     3061 ttccactgac caaaacgggg ggagaaggcc ttgagttcgg aaaaccacgc ttcaacgaat
     3121 ccttcatcga tcccttcccg ctggaagggt attatcctgg ccggaggggc gggaacgcgg
     3181 ctccatccca tcaccaaggc cgtgagcaag cagttgctcc ccatctacga caagcccatg
     3241 atctactacc ccctgtccgt gctcatgctg gcgggcatcc gggacatcct cgtcatttcc
     3301 actcccgagg acctcccccg gtttcagatc ctcctggaag acggggatca gtggggggtg
     3361 aggttttcct atgcccggca gccgcagccc gggggactgg cccaggcatt cctcatcggg
     3421 cgggatttta tcggcaagga tcccgtttgc ctgatcttgg gagacaacct gttctacggg
     3481 ttcggcttcc gggagctcct gggaaaagcg gtccggatgg agcggggcgg tcttatcttc
     3541 gcctatcgag tggtggatcc ccagcgctat ggagtgatcg aattcgatga agagggacgg
     3601 gccgtcagca tcgaggagaa gccccagagg cccaggtcaa ggtacgccgt ccccggcctc
     3661 tacttttacg acaatcaggt ggtcgacatt gccgcgggtt tgaaaccctc ggcccggggc
     3721 gagctcgaga tcacggacat caaccgggaa tatctgcggc gggggtgcct gcaggttcaa
     3781 gtcctgggca gagggttcgc ctggctcgat acgggcaccc acgaatcgct tctcgaggca
     3841 agctgtttta tcgaaaccat cgaaaagcgg cagggactga aggtcgcctg cgtggaagag
     3901 gtggcgtatc gcatgggcta catcggggcc gggcaactga ggcgccttgc ggaaccgctc
     3961 atgaaaaacg ggtacggccg gtacctgttg aggatcttgg aggaataggg gcacggaaag
     4021 ggttcgcccc ctgccggatg tcggcccttt ctccggtccc tttcgggatg tcgccggtgg
     4081 gttcgcgtat caagtgcccg gaggccgatg gggaaatcac agagggtcga ggccggtgga
     4141 tctccgcttg gagaatccgg gggcttttga aggaaggaaa atctcctgaa cgacatggaa
     4201 ccgtctgccg atctgcatcc ccgggtcttg gtcctgggta gcgggggtat gttgggacga
     4261 gaccttctgc gacgttttcg ggactcggga attcccgtcc taggtctggg gtccggggaa
     4321 tgcgacatca cccgcaggga ccgtgtggcc gcctgcgtcg agagccgacc ctggggagcg
     4381 gtgatcaact gtgccgccta caccgccgtg gatcgggcgg aggcggagcc cggtgcggct
     4441 tttcgggtca accgggacgg ggccgcccac gtggcggagg cgtgccgaag ggaaaacgtc
     4501 cctctgattc atttgtccac ggactatgtc tttgacggtc aaagtcgggt tccctacagg
     4561 gaagaggacc ctgcccgtcc cgtgagcgtc tatgggcgga gcaagtggga aggggaggaa
     4621 gcggttcgag accgatcggg gaaacatctc gtggttcgta cggcatggtt gtatgccgcc
     4681 catgggcaaa acttcgtgac atccgtcctt caccttgccc gacgaaggaa ggaattgcgc
     4741 gtggtggacg accagtttgg ctgtcccacc tggaccgaag accttgccga agggctggtg
     4801 agcatggtgc gaaaggtcct ggaatccccg aaaggggttc catggggtac ttaccacctt
     4861 tgcggagggg gcttcacctc gagacacgcc tttgcccggg ccattgtgga agagggatat
     4921 cggcgggaac acctggtggt cggcaggatc ttgccggttc ccacggacca ctatcccacc
     4981 gctgctcgcc gccccccatg ggccgttctg gactgtcaaa aagcgaacaa tgctttcggg
     5041 atcgcacttc cgccctggca aaccgggctt gcccgcatga tggaagagct gtacactccc
     5101 acggcttgat ctgcgcccca tgccccggcg cttgcattcg tttctccatt caagagatcg
     5161 cgagaacttc cgcatccaac ggacaggagc cgtgaatgat tgaaggagtg gtcgtcaaag
     5221 agctgaagac ccacctggat gatcgaggct ttttccggga aatcattcgg gttacggacg
     5281 atttctttgg ggaggggttc gcccagtgga gccattccct catgttccag ggggtgatca
     5341 aggcgtggca cgtgcaccga cggcaggtgg actggtggta cgtggccgtg ggggtgctca
     5401 aagtggctct gtacgacctg cgtcccgact ccccgaccca taaaaggctg atggaatgcc
     5461 gcatgggaga gggtcagacg gccgacgtgt tgcgaattcc cccgggggtt gcccacggat
     5521 gcaaggtgct ccaggggccc tcccaccttt tctacatcac ctccaaggtt tacgatcccg
     5581 aagacgaagg gcgtatttcc cacgatgacc cgggcatcgg gtacgactgg acgtcccctc
     5641 ccggaatccg ttaacgatcc gggaggaatc gagaactctt cggatttccc tgccgagaat
     5701 ttttccgctc ccgctctagg aggtcggctc cggagtcaag ccggggagat tcttcccccc
     5761 ccgtcccggc agcggtcgca tagctttccc ttcgagactc aggtcagaaa gttgactggt
     5821 ttctattccc aatgtccaca ggcacttcaa ggaactgcgg gaccgacagt ccgggatttt
     5881 cctccacggc cggggaaatc ttttgggatc gtcacggatc tgacgccgtt accgatgtcc
     5941 tctgcggttt tcttccaaag atgccccggt ttctcggcgt cttcttcccg cgaaaggcgg
     6001 atcgaggacc ggccttgagc cagggggttc gaattcgggg attgggaatt ggcggagaaa
     6061 tcccgggtga gactaccggc ttttgaaggc tttgggccgc tgcggtccgt ggggatgggg
     6121 catgctattt gcttaggtcg cctgtatgcc tccctaagtt gcggcaggtt ctggggtttt
     6181 tggagattgg aggatcgaac ccccgggtcg ttccggagac gacctccatc accgttctcg
     6241 gagggcaagg gaacggaggg agcgggcaga acttcccggg gggcactgta ccaggtcgga
     6301 aggaaatgaa gatatgctca acgacaaagt catcctggtg acggggggga ccggttcctt
     6361 cggccaagca ttcactcgag cggttctttc ccgctataag ccccgcaaac tgatcatttt
     6421 cagccgagac gagctgaaac aatcggaaat gcgggaacgt ttccctcggg atcaattcga
     6481 gagccttcgt ttcttcatcg gggacgtacg ggaccgggac cgcctgtatc gcagctttga
     6541 cggagtggac attgtggttc atgccgctgc attgaagcag gtccctaccg ccgagtacaa
     6601 ccccatcgaa gcggtgaaga ccaatgtgtt gggtgcggcc aacgtgatcg atgcggccat
     6661 cgatcacaat gtacagaaag tgattgcctt gagtacggac aaggccgtga gccccgtcaa
     6721 cctctatggg gccacgaaac tatgctcgga caaactcttc agtgcggcaa acagttactc
     6781 cggataccat tccacgcgat tcagcatcgt gcgttacggc aatgtcatcg gaagccgggg
     6841 cagcgtggtc ccgctcttct tgaaactgcg cacgagcggg gtccttccgg ttacggaccc
     6901 ccgcatgacc cgcttttggt tgaccctgga acagggagtc gactttgttc tggcttccct
     6961 gcagcgtatg aagggagggg aagtgtttgt tcccaagatt cccagcatga aggtcatgga
     7021 tttggccgaa gccatcgctc ccgactgccg agtggaaatc acggggattc gtcctgggga
     7081 aaagcttcac gaattcatga ttaccccgga cgacgcccgc cacaccctgg aatatgagag
     7141 ctactacgtc attctcccga cctatcgcgc cggagaactc catcattaca tggaccacaa
     7201 cgggggaaaa tgctgtcccg agggctttcg gtacagtagt gacggcaatt cctggtggct
     7261 gagcagggaa gaaatgcaac gagtcgttga acatctctgc ggaacgaaac cgggcatggg
     7321 acatggagca tgaaagccgt gggggacttg cctttcttac cgtatggacg tcaaaccgtc
     7381 gacgaagacg acattcggga ggtcatcgag gttttgaagt ccgactggct gaccacgggt
     7441 cccaaggtgg aggagtttga agcgaccttc gccgaatcgg tgggagccga atgcgcggtg
     7501 gcggtgaaca gcgggaccgc agccttgcac tgtgccatgt atgctctggg cgtggggcct
     7561 ggggacgaag tcgtcgtccc gcccatgact ttcgccgcca cggccaattg tgtggtcttt
     7621 cagggggcaa ctcccgtgtt tgcggacgtc actcccggga cccttctcct ggatccggct
     7681 cgggtggatg cgtgcatcaa tccgcgcacc aaagccattc tttgtgtgga ttatgccggc
     7741 cagccctgtg actatgatgt tttgggcgag attgcacgct cccacggaat tcccctggtc
     7801 gccgatgcat gccattccct cggtggcggc tacaagggcc gccccgtggg caccttggcg
     7861 gatatcagcg tctttagctt ccacccggta aagcacatca ccaccggaga gggaggcatg
     7921 ttgactacga accatccgtc cctggcgacc cgcgcgcgga atttccgcaa ccacggcatc
     7981 tccggcgatg cccgccggcg gtctgagaac ggttcctggt actatgaaat gacggacttg
     8041 ggcttcaatt atcggttgac ggacttccag tgcgccctgg gaatcagcca gttgagcaaa
     8101 ctccgcggct tcgttctcca gaggcaggtc ctcgccgggc actatcacag agggttctcc
     8161 ctcatccccg agatcacgcc ccttttggtt cgtcccgagg tctctcacgc ctatcacctg
     8221 tatgtcgttg cgctggatca ggacaggttg tccgcggacc ggaacaaaat ctttgcggct
     8281 ctccgagccc agggaatcgg ggtgaacgtt cattacattc ccgtgcacct tcatccatac
     8341 tatcggcaac gtttcggcac cgggcccggg atgtgccccg tggcggaaac atcgtacgag
     8401 cggatcctga gcctgccgat ctttccggca atgtctcccg gggatgtcga gactgtggtg
     8461 gatagggtcg gagatcttgt ggatcgttac cgccaccctt ctgcgcagca gtgaccggag
     8521 ggaagaaaac catgagaaaa gtcaagatcg gcccgcgaca aatcggtccc gaggaacccg
     8581 tctttgtggt ggctgaggcc ggaaccactt ccaacgggga tctccccaca gccctgaagc
     8641 tggtggacgc ggccgcagag gccggtgcgg acgcaatcaa gttcatgatt ctcggtccgg
     8701 actatttcat gagcgaccgc acggtcacgt atgaatacga gtgggccgga gggcgacatg
     8761 tggagaacat gtacgagatg ttcaagaagc tgatgttcac cgaggaggaa tggttcaaga
     8821 tccgcgacca ttgccgggag cgtggaatca tcttctacgc gacggtggat tacctccagg
     8881 gcgtggacct ggccgaacgg ctcgatgtgg cggcctataa gctcagctcc tgggacatca
     8941 cctaccttcc cctcatcgaa aaggtgggga gcacgggaaa acccgtccaa ctggacctgg
     9001 gtcccgccac cctcgccgac ctggcgaagg cgatgcaggt gctggaccaa tgcggcaacg
     9061 accaggtgat cctggtttac tgcactcatg ccgccatcga ccgtgaggtc aacctgccca
     9121 gcgtgcctta cctccagcag gtattccgtt gccccgtggg gtactcggca gacagcctgg
     9181 aaacggctcc cgatatcgcc gccgtggccc tgggcgctca cctgatcgaa aaacgactga
     9241 gcctttcccg gtcctatttc ggacaccacc acatccatgc cgtggagccg gatgagttca
     9301 aggaatacct ggcgtgcatt cgacgggtcg aggagatgct ggggcatccc caattgacgc
     9361 cttcacgcga agatacccgt ctcaaggaac actactatgt cagcctggtc gcggacaggg
     9421 acatccccgc gggtaccgtc atcacgcggg acatgattgc ctgcaaatgg cccggaaccg
     9481 ggattgctcc cgaatttgcg gacctagtgg tcgggcgcac ggcacgcagg gagattgcct
     9541 gcaaccaggt cctgggctgg gacgatgttt gagaacggaa tgcctatgcg gaaacaagcg
     9601 gaagcaaaac ggctcgaggt cttgtggacg ggggatttcg gcagggagta cactcgaagg
     9661 aactcccaag tcactccgag taccggaacg ttccaccatg ccctgggcag acagctcgag
     9721 gcgcgccgca tcctcgaggt cggctgcaac ataggtctaa acctgacccg gctggctgcg
     9781 gacgggcact ttgccgtgac gggaatcgac ctcaacgggt acgctcttca agaggcacgg
     9841 cggcggcttc cccatgcaaa tcttctgagg gctacggcct atcgtcttcc cttcgcggac
     9901 gaatccttcg acctggtttt tacctgcgga gtactcatcc atattccgcc cgagtccctc
     9961 gatgacgcaa tccgcgagat tcatcgggtc agcaggagat ggatctggtg cggggaatac
    10021 ttcagcgaac acctgaccga ggtgccctat cggggcgaaa agggagccct gtacaagtgt
    10081 gattttgggg cgagctacct cgatctgctg ccggatctcg agctggttcg gcggggcacc
    10141 ttggccaagg aaagtaccgg cttcgacaac ctggaaacct ggcttttcaa gaaaggatag
    10201 gcgggtttgt caaggattct ctttcgctgc gacggctctt cgtccatcgg gatgggacac
    10261 gtagtgcgat gcctgtccct ggctcgatcc ctgcgccaag caggggcgca ggtgttcttc
    10321 ctgctgcgcg agctgggagg aggggcccag ggcaaagttc gggagctcgg gttcgacgtg
    10381 ctgactctcc cgggtcccga ttttatcaat ggtgccttgc ccgaatggga caggctccgg
    10441 accgcagagg ccgccgggga aaagggggcc ggttgggtcc tcgtggatca ttacgccgca
    10501 agggaggatt acctggaagg actccgttcc ctcggccttc gcatcgcggt gctggacgac
    10561 atggccgacc ggacgttgac ggcatgcagt tggcttctca accagaacct gggagccgat
    10621 gcactgccct accgcaccac cgtcgaatgc gtccggctcc tgggtcccga attcgcgctt
    10681 ctcagaccgg aatttcacgc cgctcggcag gccctttctc gagagttctc ccggcgggac
    10741 gcgcacgtga tcttgaccct cggaggagga gggacggggg agctgagcat cgaggtgctc
    10801 cgagcccttg atggggtttc ctctccgctc gaaatccgct gcattcaggg agggagcgac
    10861 cctgttcccg cggagctgcg gcatgcggcc ggctcctcga tgcacaaggt ctccatcctc
    10921 cgggacgtga ggaacatggc cgcccatatg ctctgggcgg acctttccgt taatgcgggc
    10981 ggctccacct gctgggagtt gtgctgtttg ggtgtcccca tgatcactct ggtgacgtcc
    11041 gaggatcagg ccatgatcgc ccgggccctc gaggaacacg gctgtgcccg caacctggga
    11101 gaatggacgc cggggcggac ggcggcccgg ttggcggaga cggtccggga gttgatcgac
    11161 aatccggacg tgcggttgga gatgtcccgc aaggcccggc ggcgggtgga cgggcttggc
    11221 gcaaagcgcg ccgcggattc gcttcttgat ctcatgaaca agtgaaagac gggcattggg
    11281 tgacatgaag ggccttcgaa tcggaactca cctcatcggg gagcgcgctc cctgcttcct
    11341 ggttgccgag atcggcatca accacaacgg ctccccggat ctcgccagga agatgatcga
    11401 tgcggcggcg gacgcggggg cggacggggt caagttccag aactaccgga cggaagattt
    11461 catcctcgat cgctccctca cctatcggta tcccagccgg ggggggtggg tgacggaatc
    11521 ccagtacgaa atgttcaaac gctgcgaact ggaccgtaga gcccttcaag agctcaagga
    11581 acactgcgac cgccggggag tggtgtttca cagcaccccg accagcccgg caggggtgag
    11641 ggacctggtg gaaatcggaa cgaaggttct caagaacgga tcggatttcc tgacccacct
    11701 cgacctaatc gaacacatgg gccgaagcgg ggtgcccacg gtattgtcca ctggaatggc
    11761 aaccctggcc gagatcgagg aagcggtgag ggtctttcgc aaaacgggaa acgacggtct
    11821 gatccttttg cactgtgttt cttcgtaccc gacgcctccc gaggacactc atttgcggaa
    11881 gattcccgcc ctggctgcgg cttttgactg tctggtcggc ttcagcgatc acacgtccgg
    11941 aacggcggcg gccatagggg cggtggtctt aggcgcatgc tggctggaaa agcactttac
    12001 cctggatcgt tcgttgcccg gtccggatca cgcgttttcg tcggacccgg aggaatttgg
    12061 atgcctggtc cgggcggtgc gggacgtaga aaagtgcctg ggcacctcga ggttggccct
    12121 cacgaccaag gagctcgaga gtcgccggga tttccgactc tcctgtgtgg ccgccgggga
    12181 cctggcgtgc gggcacagga tccgggaaga ggacgtggtg ttccgtcgcc cgggcacggg
    12241 acttccgccc agttgtctcc ccctgctggt aggcagaatc cttcggaagg atgttgccat
    12301 aggcgaagtc attcggtggg cggacattta gtagggggta taggtgcccg gaccgtttcc
    12361 agatattcaa gtcgggctat gtttcgggca aacgcggcaa tggaggaagg ggggcgctcg
    12421 aatgcctcaa gagttttgga tcgaagaata ccgaaagaag gcagcctctg cggatcctat
    12481 cctgcagtcg gggcgaggca agcaattcga tgcagtggag cttttatatg tggtcaagca
    12541 ggtcgttgag ctgctgagat tggatgccag ccatgaactc ctcgatgtgg ggtgtggcaa
    12601 cggattgctg gacattgttc taagcgcttg ttgtcggcac gtgatggcca tcgagcccgt
    12661 tgaggagcta gcggagttgg cccgcaagaa cctgaggcac tgtcccaatg ttgccgtcga
    12721 ggtcggccat ggggccgaca tccgtgccgc caacgacact ttcgattgca tgctcgcctt
    12781 cggcgtcctt cagttggtat cgcccgagga ggccatcgag atgttcgggg aagcagttcg
    12841 ggtagtgcgg cctggaggtc gagtggtttt cggttccatc ctcgacgccg cgcgacggga
    12901 ccggttcttg atcccttacc tggatggcgt ccggggtggc tctcatctct ctgccgatga
    12961 gaaagaggag atcatcaggc gtaacctctc tgcccattgg catcacccca aagacctcgc
    13021 ggattggtgg atggggcgag ggtgctccgt gacaattcat tcgcttgttg ccggggatcc
    13081 cgatgcagat catcgctttc acctggttgt tactgtggag aagcatgcat gaggatcgtg
    13141 ttcctgggca atcacaccgt gggcgttcgc gccctggaag ctttgctgca aaaggatgag
    13201 gtggttggag tagtggctca tcctcccgat cccgaagacg ggatacggta cctttccgtg
    13261 tacgatttct ctcggaggag ggggctgcac gcggtgagaa aaaacggtcg ggaccccggg
    13321 ctcgaagagt tcgttcggga ctgtcggccc gatctcctgt ggatcacgga ttaccgatac
    13381 ctgttgcccc agggagtgct cacggttgcc cccctggggg tggtgaacct ccatcccagc
    13441 ctgcttcccg cctatcgggg aagggcaccg atcaactggg cgattctcca tggtgagcgg
    13501 gagctgggcc tcacggtcca ttttgtggac gaaggcatgg ataccggaga catcattgaa
    13561 caggttccat tcttcctgag cgaaaatcag gatgttggcg acgccctttg cattctctac
    13621 cccctgtacg gcgagatcac taggcgggtg attgctcatt ttcggaaagg aaaggtcccc
    13681 aggcggcctc aggatcatcg gcgggcgacc gtctttcccc gtcgtactcc cgaggatggg
    13741 ctgatccaat gggagcagcg agccgaacgc atacgagacc tggttcgtgc ggtggctccc
    13801 ccgtaccccg gggccttttc cttttgtcac ggcaggaaga tccgaatcct ttcggcggcc
    13861 gtgcggtccg aggagggagt ccacggcgaa cccggatcga ttttgagtct cgattcaacg
    13921 ggtccggtgg tttgcgcggg cacagggacg gtttggatca gaaaattcga atgggaaccc
    13981 gccgacccgc cggggaggct caatcccgga gaccggatgg aaaaccgatg aaatctcccc
    14041 aagaacaggc agaagaccga aacatcgtga actattacac cgagctcctg gaaaagcacg
    14101 gccggaaccc ccggtcgttg gactggggca gtcggcgaac tcaggaactg cgtttccggg
    14161 ttctttgcga catcgctccc ctggacggct gcagcctgtt ggatgtcgga tgtgggttgg
    14221 gggacctttg ggactacttg tcatcaaaag gcatcggagt cttctacacg ggatacgact
    14281 tcacccccgc aatggttgcg gaagccaaag gccgttttcc ctcggtgccg ttcgaggttc
    14341 gggacatcct cgaggagccg gatgagcatt gccggttcga ctacgtgatg gcgagcggga
    14401 tcttctactt gaggcgggac cgtccgatgc agtacctggt cgaaaccgtt cgcaggatgt
    14461 tcggcctgtg ccgcatcgga gtggccttca acagcctgag cgctcgggct tcccgtcgcg
    14521 atccccatga gttccatgcg gacccatcca gggtgctttc ttcctgcttg gagatcaccc
    14581 ccagggttgt gctccgacac gactatctgc ccaatgattt tacggtctat cttttcaggg
    14641 attgaggcct tccatgatcg tcaccatcag tcaacccagg tatcttccgt ggctcggcta
    14701 ttttcaccgg atcgccgtga gcgacctttt catctacctg gacacggtcc agtacacgcc
    14761 aagagattgg gaaaaccgga accggatcaa gaccagccgg ggtccgacat ggctcacggt
    14821 gccggtcaaa gccggatatc gcgccatgat cccggaggtg accatcgaca acgaccagcc
    14881 ctggcaggcg aaacactgga agaccctctc catgaactat tcccgtgcac cttatttccg
    14941 cgagtacgag caggcgctgg gggagatcta ccagcaaaaa acctggccgg aactcaccgc
    15001 cctcaatcta cgttccaccc ggattctgtg tgagcagctg ggactggaca aggcccgatt
    15061 tgcccgggct tcggagatcg gtgccccggg aaagggctcg gagctcattt tgaacctttg
    15121 tacagccgta ggagccaagg tgtacctctc cggatcccag gggcgcaact acctcgatga
    15181 ggagaagttt cggcaagcgg gaattcgtgt cgtataccag gactatgtcc atccccaata
    15241 cccgcagcta tttggaggct tcgctccttt catggcggtt cctgatttgt tgttcaattg
    15301 tggccctggg agcctcgata ttctcgtgac aggacagaag gaggttttac gatgacttct
    15361 cttgagcgga accctctctg tatcatccct gcccgggggg gatccaaacg attgcccaga
    15421 aagaacatcg tccctctggg gggcaaaccc ctgctgactt ggaccattga cgcagcgttg
    15481 gaaacggggc tcttcgatcg agtgtgggtg tcgtcggaag atccggagat cctcgaagtt
    15541 gcccgagcgt gggggggggc gccgttaaca agacctcccg agttggccga ggatcatgtg
    15601 agccttgagg agatgtgctt gtgggcgatc gagtctttgg cgtcccgagg ccatgactat
    15661 acggatcttt acatgcttct tccgacttct ccccttcgta aaggctcgac catcaagcgg
    15721 gcgtggaatg tctaccttga gagcggggcg catttcctgc tcagcaccat acctctccat
    15781 catcctcccc agtgggcctt ggttgagaag aacggtttgc tgagtccttt gttcccagag
    15841 gaatatgaga ccccccgcca atggctggta ccttccttgc gacatgaggg gggccacgcc
    15901 atcggaaaga tctctgagtt tttcaagacg cgatccttca ttagttcccg aaccgtcgca
    15961 tttccggttg cagcggagga agcggtggat gtggatgaac ctatggacct ggcatgggcg
    16021 gagtttctat tgcaacggac ggcgaacaag aggggagatc atgaacgtgc ttgttgtagc
    16081 ggctcacccc gatgacgaag tcctcggctg tggcgggacc atcgcccggc tgtcacgaga
    16141 aggtcatgcc atcgacgtgg ccatcctggg tgaggggatc acctcgcgct accgggcgag
    16201 agacgacgct gacgccgcca ccgtcgaagc gctccacgaa cgcagccggg aagtggcgag
    16261 acttctcggg gtccgggacc tttcccttca caatcttccc gacaatcggt tcgataccgt
    16321 cccactcctc gacattgtca agatcgtcga ggacctggtc tcccgcttca ggccggaggt
    16381 tattctcact caccacggcg atgaccttaa tgtagatcat gcgattgcct ttcgtgccgt
    16441 gttgacggcc acccgtcccg ttatcgcaat gccggtcaaa gaaatccatg ccttcgaggt
    16501 accgtcgtct acagaatggg ctttcggccg gttgcaccgg ggctttaggc ccaatgtgtt
    16561 tgtgaacatc acgaccacga ctgcggcgaa gatccaggcg atgcgtgttt atgagagcga
    16621 agtgcgggcg tttccccacc cgagatcccc tgaggccctc caggccctgg cccgttaccg
    16681 aggcagcagt tccggttgtg aggcggccga ggctttcgag ttgattcggt gggtgcgcta
    16741 gagggaccca ttcgccaaga gcacggcagg agatgtccat tactatcttc aatgctatgg
    16801 gaaggtgagg gcgcttgggt gtttgtcatc cactccctga ttcggagttc acaaccttag
    16861 attactgaag ggcggtctcg tcggtttacc cgtttttatg gagatatgct tgtcgatgtg
    16921 gccccggtac agaagattca aacttctgga atcgaaggtg tcagaaggta ggacttggca
    16981 gcttccgcaa ctcacggaga agaaagtgct acctgcttgt gcacgatagc aaatatgttt
    17041 tgtgtggctt gcggaagcag atggatggga gtttgcgagc ttgattccac cgggcatcgg
    17101 gtttcaacat aaagaataca ttaggaggca actactcccg aattgcgctt gccatcagtg
    17161 gcattctgag tgtgtctctt acgtatgaac ctacaaaacg ttatgcaccc atacggaaaa
    17221 aaaggaaaaa ttatgtcaaa ccgaaaaagg gtgttactag tttcttacta cggcaaagtc
    17281 tgcctgagta ccaaaatcct ttcgggcatt ctgcggaggg ctgggcatga aacgtgcctc
    17341 atctacctta aggatgatag aagcactcag gtcgagtccg tcaataaggc aagctcttgc
    17401 ttccagttca tatgttccga tcggggtgcg gcagtctatg gtacagggat ggacgtcaat
    17461 cccataaggt caacggaact cgatctcttg gtaaacaaag cactggagtt cctgcctgat
    17521 gtggtggcca ttagttcgcg ctctgttcat atcgaagtca gtcgggtaat tatcatgctt
    17581 ttgcggaagg tacttcccaa agctaccagg tatattgcgg gcggatatgg ccccagcctt
    17641 gagccggaga aattcttgga agtgttcgat tatgtttgca tcggcagagg agattctgct
    17701 gtgttggatc ttgtggaaaa agaagatccg agttcctgca ggaatgtagc taccctagtc
    17761 gacgggaagc ttcatatcgc tttccttgat aatcaagtgg atttgaactt gagaccgttt
    17821 gccgattgga attttgaaaa caaattcttg attgaagatg gaaagatttt cccgctacat
    17881 gaaaagtatg atccgaaaac ctatggcatt atcgcttcgg agggatgtcc ttcctcctgt
    17941 acttattgtc aggcctctca gtggccccat atctaccgac tccatggtgg caggagtccc
    18001 aaagtggtga tgcgcaaacc cgacaacgtt attgccgagt tgattcacgc taaagaccat
    18061 ttcgggatcg agagtgtaca gtttatggat tcgatcttca catggcaccg gaaatggttt
    18121 gatgagttca tggccctgta taacagacat gtggcgctgc cgttttgctg ctacacggat
    18181 gcccgcttca caagtcgcaa gcagcttgtc cgcctgaaag agtccgggat gttttccaca
    18241 accgtaggca tccagtcggt ggatgaagag tcccggagaa aggtaatggg gcgcagagtt
    18301 tcgaatgaga ggatcattga atttgcatgg gaggttgtcg aactcggtat tgtgccaacc
    18361 tacgacatta tcgtatggaa tccgtttgaa actgaagaaa gcctggcaag aggtgttgat
    18421 ctgcttaggc aactgcctaa atcaaacaga atcttcattt atgaactcaa gattctgcca
    18481 agaagcctat tggaggatct tttcaacgag tgcaggccga cacccctcag tcaggaatcc
    18541 tatatgtatt ggtcttggat atttgtcatg ctcttgagaa gcgatgcgac ccaagaggct
    18601 gttccggcga ttttgtcgaa cccaatatac aaggattccc cacatctcat ccgcgagcgg
    18661 tatctggaga ttctgggttc ccaggaagta cgtgaacgtt tgtttgccat taggccggtg
    18721 gctatgggtg aaatgcttag accgaccatg ttcatcgaaa aagcaagcct caatttggaa
    18781 ggtgtgttgg gggaggatcg ccactacatt actgggaaaa cgattcgcag gcctctccga
    18841 accggcgaag tgctttgcta caaggacttc tatacatcct accaggaaaa atgacctttc
    18901 atactttcag gagagggata tgctttttcg gagtctctgc aatgtgaaga gaatcatgac
    18961 agagccatcc gtcaaccatg atcccgggga gcaatccaaa catgtgcacg tccatggaga
    19021 tcggccgagg atgtgcgctt tcgatttgaa gatgaaatcc actgatccaa tggagagact
    19081 ggtaccatgt cgacgttagc tcagatacgg cctccaaacg agagtcccag agtgctgtgt
    19141 tactcccctt acaactattg ggaactccac gggttgtggg agataaccat tcttcacgca
    19201 ctgagaatca gatcggccgc cgtgcggtat gttctttgta atgggatcta ttcggattgc
    19261 gatatgtttt gggcagctac ccgcccaaga acctcgtcat cctgtactga ctgtcagaca
    19321 tttgtttccg acctcgctaa gcggatgcaa atgccgttcg agtggcttgg agctcaatta
    19381 tctccggagg atttccttca aggacgatcc tgggcagacc gtctgcggcc ctcggagttt
    19441 tcgcacgcca cttacggcga gtggaagatc ggcgagtggg ttgaaggatc gattcactcc
    19501 catttccgga tgagtgatct cgatttgtcc ttacccgaaa tgatccacac ctaccgtagc
    19561 tacctctaca gtggttccat tgccgccatg gcgctttccc ggcttctcga cgagttccgg
    19621 cccgattgcc ttttcctgtt caacgggcgg atgtcatcca cccgtatcgc tctggaactt
    19681 gcgcgcatgc gtgacattcg ggtaatcgtc catgagcgcg gaaggctcaa ggaaagctta
    19741 tctcttcgtg aaaatgcaaa tggtgcctcc ctaagaccta tgaaggaagt atgggagaaa
    19801 tggggcgaga tcccgctctg tcgttcagaa ctggagacca ttgaaaaatg tatgctagaa
    19861 cggcagcagg ggttgaatca taactggcgt gttttcagcc catcagagca gaatcgggat
    19921 gaggttgtcc gaaccctaca attgaattcc acgaaacagc aatgggtctt gttcacttca
    19981 tccgatgacg aggtcatagc ggcaggggaa tgggcaggcc cgttcaaatg tcagatgaac
    20041 tgggtgctgg ccacgctcca ttttgtgcga agtcacccgg agattgagct gatcatccgg
    20101 gttcatccaa acaccggtgg tagcaggtcc accggggtca acgtccggca acttaaggaa
    20161 ttcgagtgtc ttgcccggga ccttccgtcc aacgttcgga tggtcatgcc ggacgacccg
    20221 atcagttcgt acacgctgat ggacctggct tcgctgggtt tggcatatca tagcacggtg
    20281 agcctggaaa tggcctgcaa gggaaaaccg gtagtggtat ccgcaggttc ggtcgtgaca
    20341 ggtttaccct ttgtcctcac accgaatagc cctacagaat atatttcaat gctgagttcg
    20401 ctgatccaca gcgacaaagg ccgaaacgct ctcgaaattc aaagacaagc gtatcggttc
    20461 gcctatggtc ttttctaccg gatcaatatt ccctttcccc ttgtgaccat gccaaaccct
    20521 cacaaaggca tactggcata caagtccctt gaagacctgc tgcctggccg tgaaccgaac
    20581 ctggatcgaa ttgcggggat tattcttgac aggcagcctg tttgtccaga accgacgtca
    20641 gaggagctgc aacggtctcc ggaggaggaa gatgcctggt tcgaaaagag ggacaaccgg
    20701 gtttccgtca ggatgactcc tcgtgtggga ggtcccttgg tttcagtcgt cattccttgt
    20761 ttcaactacg ggccgtatct gacggaagct gttgaaagtg tgatcgggca gagctttcag
    20821 gacttcgaaa tcattattgt gaacgacggc agcacagaca attcacgcca ggtcgccgag
    20881 gatctgatcg ccaatcatcc aggacacttg atccggctca tcgatcagca taactccggt
    20941 caacccgcag tttctcgaaa caatgggatt gcagaagcac gagggacata cattctcccc
    21001 gtggacgccg acgacaagct ggcaacatcc agtagcctca aaattcttct tgacgaagcc
    21061 atcaagcacc gggacacccc tgtggcagta gtagggtggg tgcaaaaatt cggggtagag
    21121 gacgatttgt ggaaacccaa ggggtttgat ccgaacagga tccttcgccg gtgcaccgtt
    21181 ccctactgtg ccctgtatca tcgatcggtg tgggaaaggc aaggtggata ccggaccaat
    21241 gttcctggtt atgaagattg ggatttctgg attggggcca cgattcaggg ggtgcgattt
    21301 gtccatttgg aagctcccgt cctcctttac cgcaggaccc aattcaactc cctcgtagac
    21361 aaagctcgca tgagacacga atggttgatg gccggaatca tcacaaacca ttccagtgtg
    21421 tatgaggcat ccgaggttgc ctgggccgaa gactaccgcc gacgccaccc ccaaccgccc
    21481 aaagaacgaa ggttttcgga agaaatccag cgaaatccgg aggtagcggc agcactggtg
    21541 gcaagctatc cggaacacta tgcggaggac gaagtcaagt gggccatcca acaccttcac
    21601 cgtcacccgt tcctaatcca caaggggctt cccgtggccc cgaactccgc tggaaagaag
    21661 atcttgatgc tgcgggggga tgtggtgtcg acgccgaccc aactggccct ggcttggttt
    21721 cgagagagcc tcggtgcgct tcaggagagg aacttcccgg aagcggagcg actgatgggg
    21781 ttgtaccgtc agtccattcg ctatgagacc ttcaccgcca gggacggccg tcctgagcag
    21841 cttccgggaa tttcggtgat cattgttgca tatcgcacaa agcagttgct gctggagtgt
    21901 ctgaactccg ttttcgagca gagttatcga gatttcgagg tcattgtggt ggacaacggc
    21961 ggcaatgagg aagtccatga aaccctgcgc tcttatccga tccttcacgt acaatgcccg
    22021 gataacctca tcctgtcaga ggggagaaac atcggtgtgc accatgccag aggcagaatt
    22081 gtagccttcc tggatgatga cgcccgtgtt ccgcgcaact atctggagag catcgacctg
    22141 gccttcaagg cgtatgatat cgtcggaatg agagggaagg tgcacccgaa gtccactctc
    22201 tcccatcact cccatgccgg tcactatgac ctgggggaca cccctattcc ttcaaccatt
    22261 gacacggaag gcaattccgc gttctccagg aaagcctatc aggaagtggg gggtatgaat
    22321 cccctgcttt ttggaatgga aggactcgac ttatcgtatc gactggcggg caggtatggt
    22381 ccacatcgca ccgtttattg gccggaaacc ctcatttatc atgactttgc agtgaccgag
    22441 gaaaaggttg agaaaaaact ggcgcaacat gctctgatgc gagagtacct gctttacaag
    22501 tttccggata tctatgattt tcataaccgc ttgagagggc atgcggcaaa cgagtcttcg
    22561 aagctgcacg gcaaagctct gataccggca aagacttgct ctgccccggt ttccatcgag
    22621 accgaggcca ctgtgacact tgccgttgtt acgtataatc gtgccaaaac cattggtcgg
    22681 gcccttgaga gtgccttgaa tcagtctcgc cctgcggatg aaattctggt cgtggatgac
    22741 ggatccgagg ataagactgc ggaagtggtg gctgccttcc gaagcccgcg tatccgctac
    22801 atcagaaaag agcactcggg tgcgccgcaa actcggaatc gagccgtggc ggaagcaagt
    22861 ggagatttcg tcttgtggct ggattccgac gactgccttc caccccacac cgtggcgagt
    22921 cacctggctg ccctgagacg gactcccgat gccgatgtac tgtacggaaa tctactgatc
    22981 gtagatgaag acctaaagcc acagaaagag tggatttacc cggattacta cggatgcaac
    23041 aaccgcctcc tggcgagaat ggtgcgagga aattgcctgc ccaatccggg aaccatgatc
    23101 cgtaaatcct gtttttgcag atttgggggt tatgatgagt cctttcggcg tgcccacgat
    23161 tatgaattct ggagtcgttt ggcaaaagat gcggtgttca aacactcagg tgccgttgtc
    23221 tgcaactggc gcgttcatgc aaactctctc tcggcacatg gtcaaggtcc ggaaaccagc
    23281 tatgaggcaa gagtcgtcca gggtatgctg aagcggtatc cgctggagga cctatatccg
    23341 gacatcatgt cggcgatgga agatcgtgac gaggccaggg caacctgtta ctggcaggca
    23401 gcgtcgacct ttctcgagtt caaggatatt gagaacgttg tgacgtccct gcaaaagagc
    23461 tacgagtatt gccccagcga ggaagtccag gcgcttcttt cctatatcac gaagggaaaa
    23521 tcatcccatc catcgggact gccgggatcc gtgattgctg cgttcaaccg ccttaagcac
    23581 cctcccgttc ctgatcctga agatttcgat gaggcgtatt acctggagtc ctacccggat
    23641 atagcgcagg ctgtcgccaa aggagtattt cgctccgggt gggaacactt cgctcgtttt
    23701 ggccgtttct ccaaccgcct gctggccagg acgacatgga atctatcctt ccacgggtcg
    23761 tccggcgcat cgccgcccca ggtatccgtc gtcatccctt gctacaatca cgcacattac
    23821 cttcgggagg ctgttcaaag cgttttgcgg cagacctatc gggactggga gatcctcatc
    23881 gtcaatgacg gcagcaccga tgacaccggc agggttgccc gatccctgat tcaagaacac
    23941 gggtcccgca gaattcacct catcgaaaag gaaaatggtg gcctggccga ggccagaaat
    24001 gtcggggttc ggaaatcccg gggcaagtac attctccccc tggatgcgga cgatctcctc
    24061 gagccgacca tgatcgagga gtgcgtgagg atcctcgatc agaaccctgg tgtggccata
    24121 gcttataccg acgtgaggca attcggcgcc gtagagcggg tggtaccgtc cgtcccttac
    24181 gatttcaaac tcttgtgtca catgaatttc atgtgttgct cttccctgta ccgccgggag
    24241 gccttcgacg ccgtgggcgg ctacaacccc aacatggtcc ggggatacga agactgggat
    24301 ttctggatcg gttgcggaga aaaaggcttc tttggagagc atgtccccaa ggcccttttc
    24361 ctgtatcgca gaaacgcgaa cagcatggtg accgagtcgc agaagtacga ccgcgagctc
    24421 aaggccagga tcgtcctcaa ccacccggcc gtcttcagcc cggaacagct ttcctgggcc
    24481 aaaggggttg aagcgaaaga tccccggatc atggccctgc ccgaccaacc ccacttcatt
    24541 ccccttttcg aatcgacccg gcgttctccc tccgctgggg aagcaacgag cgggagagag
    24601 acgggcatga aggtcctgtt caccatgtac gggtggaacg atacgggagg aggcaccacg
    24661 tttcccaagt ccgtggcccg ggaactggtg caaagaggtc atcgggtctc ggtcttttac
    24721 gcctccttga aaaacgaccc taccatgccg ccctattccg tcctgaagga ggaggaggac
    24781 ggggtggtcc tctacggggt gtacaatcga ccggccccgt tcgtggaccc ggactgcccg
    24841 gagagggaga tccgggacga gggcgtcctc gagaggtttc gggaagtcct ggacgaggtc
    24901 ggacccgagg tggtccattt tcacaatttc cacggcctca ccttcgccct ggcccgggag
    24961 gtccgggaac gaggaatccc ctcgtgctac accccccaca actaccacat gatcgatccc
    25021 catttgtacc tgttccggag cgacctgtcc ctctggagga acacggatat cctcgagaat
    25081 tcggaagcgg tgcaacgtaa tcccgggaag cgggaactct ataaaaaacg ggcggaaacc
    25141 acccgaaagc tcctcaacga gtgggtggac atgaccctgg cggtctcttc ccgccagagg
    25201 gagctgctcg tgcagcacgg ggcgagaccc gaccgggttg ccgtggtcca ccagtcccac
    25261 gcctcgacgg atgtcctgtg gagcagcccg gagctggctg agcaagggag ccgaccgttg
    25321 catcatcctt tgcggatcgg cttcatcggc ggtgtcatgc ctcacaaggg cgtgcatgtc
    25381 ctggcggcgg cggcccaaca tttcgattcg tcccgagcgg agtttcacgt ctacgggttc
    25441 ggcagcatcg agtacatacg ctttgtgcga cgcatggatc cgggcgaaag gttggtcttt
    25501 cacggcaact acacccacga catgctcgaa tccatcgccc gggaactgga cgtcgccgtg
    25561 gttcccagcc tgtgggaaga ctgcgcgccc ctggtcctgc tcgagctcat ggccatgcgc
    25621 ctgcccgtcc tgggggcctc catcggaggc attcccgatt tcgtgaagga aggagtgaac
    25681 gggtttctct attctcacaa ttccgtggaa tccctgtgcc gatgcatcga gtactgcatc
    25741 caaaaccgcc caatcatccg aaagatgcgt tcttccctgc gccggcccca ctcctttcaa
    25801 ggctatgtga atcacctgga aaccgtttat cgcgaccttt cgagccgcaa gatcggtgat
    25861 gcctcccggt atgatcttct cgtccggctg ggttccgtct cttcccgacc cgccattcgc
    25921 tgggagggtt cccagttcgt catacattcc ct
//
LOCUS       DAOOVC010000047        25794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1277_length_25794_cov_6.121023, whole genome shotgun sequence.
ACCESSION   DAOOVC010000047 DAOOVC010000000
VERSION     DAOOVC010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 25794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25794
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1277_length_25794_cov_6.121023"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="PLM79_08155"
     CDS             complement(<1..1115)
                     /locus_tag="PLM79_08155"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV86321.1"
                     /translation="MTQSALDALVHRLTRQDMPIFAQSMKNIQGLSERRDSSLSELAH
                     VVLQDAALTARMLKLANSVHFNKGFRRINTISRAVVMLGYDTVRSLCLSISLIETLLN
                     GSLRDQVVREMATAFHSAAQARKLISRFNEKYTEEVFIAALLYRIGPIAFWCFGRDLA
                     NRLEDALKLDAGTPPARIEQEVLGFRLQQLTVRLSKEWHLSDLLDAALERKDTADIRV
                     RAVDLGYRIAHNVQHGWDTPEAAAVIRNVAEFLNLPEAEVVPLLHETAHEAFEIAASY
                     GMAASSRLIPLPREAGLVPSGTTSPDEFSPESESPVSSPAFPIPDPVLQFNVLRDLST
                     LMQDTRIDVNLLFSVLLEGIYRGIGMDRVLFAVLTPDR"
     gene            complement(1425..2129)
                     /locus_tag="PLM79_08160"
     CDS             complement(1425..2129)
                     /locus_tag="PLM79_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV86322.1"
                     /translation="MIPSLVFLFPAYRPPPVLLDLVVGTARADSSHSIIVVDDGSGAE
                     FTDLFRTLETVCNVTVLRHSRNQGKGAALKTGFRYALDHFPRAVGVTTADADGQHRLQ
                     DILHVGRTLESHPAILVLGVRDFSRKVPFRSRTGNSMSRRLWKRITGQSLEDTQTGLR
                     GIPMSVVPAILSLPSSRYDYELEVLATLTRLHLEVRPVPIHTIYPEGNRSSHFRLIED
                     TLLISKVFLRCRLRRL"
     gene            complement(2355..3014)
                     /locus_tag="PLM79_08165"
     CDS             complement(2355..3014)
                     /locus_tag="PLM79_08165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOV86323.1"
                     /translation="MRIFEYRGQKYEVDDEDFLLHPPDWNENFAEGMAERLGMELPLS
                     DRHWQIIRFIRRIFNKSGKCPMVHETCKTHKLKSADLNRLFPSGYLRGACKLAGLSYR
                     AEQVHPAWAARHNGETVPSPSEDKVYRVNVRGFLVDPAEWDEEFAVYKARELKMTKEL
                     TEKHWRIIRFLREQFRQTGFVPTVYETCAANQVEIDELAKLFPDGYHRGAVKIAGLKA
                     V"
     gene            3252..3761
                     /locus_tag="PLM79_08170"
     CDS             3252..3761
                     /locus_tag="PLM79_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOV86324.1"
                     /translation="MEQKGEPVPTIEDVKRYLRDQGIEVWEFREATPTSEAAAGAVGC
                     CVGEIAKSVLFLVGGEPVLVVTSGDVRVKSSRLKQAVGLAGRVRFPNAEEVERYSGYL
                     PGGVCPFLLPANLRVLVDSSLRRFPRVYPAAGNDHSAVPISVDRLLELTGGEEADVCE
                     AAPGDSRRP"
     gene            complement(3773..4312)
                     /locus_tag="PLM79_08175"
     CDS             complement(3773..4312)
                     /locus_tag="PLM79_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV86325.1"
                     /translation="MKPKRLSPVVLLLFIFAVEVCLSAPADVGFCQDNPHIVTRDGED
                     LLVGRISREELITDNLLFSLNAEEYVPAAKVLEKLRRITVQTDILLFLGTWSEDCLME
                     APKLLRVYDKTNNPAFALAVYALDRSLRDEEGVAARYGIRELPTVVLVRGGKEVGRIE
                     GAARRSMEADLLSILESSR"
     gene            4733..6571
                     /gene="treZ"
                     /locus_tag="PLM79_08180"
     CDS             4733..6571
                     /gene="treZ"
                     /locus_tag="PLM79_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HOV86326.1"
                     /translation="MHIGARIVEEDTGDFTVWAPTLDSVELVLLSPRAGVFPMEKGEG
                     GYWRCRVPGIGAGTRYRYRLDRDVQRPDPASHCQSEGVHGPSRVVDHGAFAWDDGQWR
                     GIPPERMVFYEIHVGTFSKAGTFAGLLERLDELADLGVNGLSLMPVAQFPGERNWGYD
                     GVYPFAVQGSYGGPEGLKELVNACHQRNMAVFLDVVYNHLGPEGNYLGQFGPYFTSRY
                     RTPWGDAVNYDGPYSDGVRNYFIQNALHWFGNYHVDGLRLDAVHAICDLSARPFLRQL
                     AEAVEEFSRQQGRKFSLVAESDLNDASLIRPRALGGRGLDGIWCDDFHHALHSLLTGE
                     RDGYYEDFGKTEHLAGAYREGFVYSGQYSRHRKRSHGSSCRQCSPSQLVVFAQNHDQV
                     GNRVQGERMSALVSREALKLAAGAYLLSPFVPLIFMGEEYGEEAPFLYFVDHSDPDLI
                     EAVRRGRREEFRDFHREQPPPDPQSPSTFLRSQLCWEKRREGPHGVLLQWYRRLIALR
                     KQIPPPASFSPEHWRVEWEERGRSIWIHRREGDRERLLAMNFGTEPFALPFPFRGRWT
                     KVLDSSEECWMGPGSDLPSSPRSGQTVCLRPFALALYEKTDGRDFP"
     gene            6568..8994
                     /locus_tag="PLM79_08185"
     CDS             6568..8994
                     /locus_tag="PLM79_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOV86327.1"
                     /translation="MMRYVCFHGHFYQPPRENPWLEDVELQDSAYPYHDWNERITAEC
                     YAPNTASRILDGERRIVDIVNNYSKISFNFGPTLLAWMERHQPEVYQAILDADRLSRE
                     RFSGHGSAVAQVYNHMIMPLATLRDKYTHCHWAVRDFQRRFGRDPEGMWLPETAVDKE
                     SLEVLADLGISFTILAPHQAGLVRLLGESNWRDVGGGRIDPTMAYLCLLPSGRKIHLF
                     FYDGPIAREVAFGGLLNNGEALADRMLGAFSPERAHPQIVHVATDGETYGHHHRKGDM
                     ALAYALYHMEANETVRVTVYGEYLERHPPTQEVEIVENTSWSCIHGVERWRAHCGCSS
                     GSRPPWNQQWRAPLRRAMDQLRDWVTPYFEREASNYLADPWKARNEYIDVVVDRSRES
                     VEAFFRRHAVGEISPEIRTRLLKLLEMQRHTMLMYTSCGWFFDEISGLEATQVIKYAA
                     RAIQLARETLGTDFEESFLEILEEAPSNIPELGNGRRVYERYVKPSRIDLPRVAAHYA
                     ITSLFEETQESALIDSYRVERTSHESMQAGVLKLEAGRLDIRSEVTREETRLFYAALH
                     LGGHNVTCGVLGAEEENRFHQMSEEFEAPFARADIPELVRLMDRTFQGNLYTLWHLFR
                     DRQQKVLEQVLAPGLEEAEFLFAQMVERNYPLMNFLQELQRPLPRHLEVAAEFLVNTD
                     LRKIFQSKEPDLDLLERRIHEARRWSVHLDSNALGFGVEMKLSRIMAEFAEDPADLER
                     LERIERLFRLLNESGLDLDLWEAQNRYFAVARSAFPEVRGRAGEGDARAAEWTRVFLD
                     LGDHLGVGVP"
     gene            8991..11822
                     /gene="treY"
                     /locus_tag="PLM79_08190"
     CDS             8991..11822
                     /gene="treY"
                     /locus_tag="PLM79_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HOV86328.1"
                     /translation="MIRTPLATYRIQMNREFGFQALNEILAYLAALGISDLYLSPILR
                     ARKGSAHGYDLVEPRELNPELGSEKDFRRLVEGVKEHGLGWIQDIVPNHMAFDPDNSM
                     LMDVLENRENSNYYPFFDIDWNHPHDDLKDHVLAPFLGRFYGECLDEGEIRVSYGPDG
                     FKVHYYDLRFPLKVESYSEVLTPNLGTLKKDLGEEDPDFIKLLGTLYVLKTLPGREAR
                     DERYGQIRFIKRVLWELHTTNLRIREHVDGNLERLNGREGGPNRFAALEGLLAQQHFR
                     LAFWKVATEEINYRRFFNINDLISLRMEDKKVFQETHALVLKLVKEKVFTGLRVDHID
                     GLYDPTQYLSRLRQEARGTYLVVEKVLDRDESLPDFWPVQGTTGYDFLNRVNELFCRR
                     EHEGRFGRIYARHGGTGQPFVDMVAEKKRLFIARHMTGDIDGLAHLIKSISGRTRQGS
                     DITLYGLRRAMTEVMSYFPVYRTYLSGKTLREEDLAAIRQAAAQAADRVPALTYELSF
                     LQDLLSLNLDGSLREEDRDRWVHVIMRFQQFTGPLMAKGFEDTTLYVYNRLISLNEVG
                     GNPQRFGVSGDEFHEYMGLRAARQPQSMNATATHDTKRGEDVRARIHVLSEIPGEWEL
                     CLKKWSQWNRRNKRRLGGVEAPDKNDEYFLYQTLVGAAPFGGPWGREFRDRMEAYVIK
                     AVREAKVHTAWIKPDTAYEEAYLDFVRTLLTPSPRNPFLEDFPPFQRKIAFYGVFNSL
                     AQTLVKITAPGVPDFYQGTELWDLSLVDPDNRRPVDFAARSSSLADLESAWRRNPAHL
                     LADLLTCRADGRIKQFVIWRGLQDRRERPDLYRKGGYLPLRVEGGRKDHVLAFARQYR
                     NEWAVTVVPRFLTSVVEAGRDPLGDPVWQDTRILLPQEAPVRWRNEFTAQSLYGENNA
                     LPLGRVLERFPVALLGQWRRKARTVSNRT"
     gene            11832..12479
                     /locus_tag="PLM79_08195"
     CDS             11832..12479
                     /locus_tag="PLM79_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HOV86329.1"
                     /translation="MADPFAVKDCALIAIATGEHAQNLREMRDHLLTVHPGCIYHHFW
                     GGMLQPRFDEPEYMNDFAAWAWRGLHDPGLAERLALIDPTHYRDLEELRRELLEVIEE
                     RLDETEMVPWSRTGQAFHFIRSRIVVFDTRIRISHPRELRDRIGQLPLGSVFYHFVDA
                     RRRAPIGKNDFSAWLMSLDGDWSELAEILGNVDPYFTTLAELRREFEEVLKAYAV"
     gene            12469..13746
                     /locus_tag="PLM79_08200"
     CDS             12469..13746
                     /locus_tag="PLM79_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV86330.1"
                     /translation="MQCEGIQVNGLLQKYAQIVGEEVVDHLRQLAEPLRGLKVVHVNS
                     TREGGGVAEILSWLVPLKNELGIHTEWEVVTGSQEFYQCTKGFHNALQGNRVEIPGSL
                     LRAYEGANEENADRLRSSLEEADVVFIHDPQPAPLLRLCSGRRGKWIWRCHIDLSRPY
                     RPVWKYLRGFVEGYDASVWSLAQFAQPLPHPLYLIAPSIDPLSEKNRELTPDEVLGVA
                     VRFGLDPKRTAITQISRFDRFKDPVGVIRAYRLCKEYTPLQLVLAGGGATDDPEGEQV
                     LREVRTAAGDDPHIHVLLLPADSHREINALQRFSQIVVQKSTREGFGLTVTEAMWKGK
                     PVIGGDVGGIRLQVVNHHTGFLVNSPEGAALRIRYLLKHREHMLEMGRKARQFVRNNF
                     LLTRHLREYLTLVHAIRHEERERIELNGLQSGL"
     gene            15311..16795
                     /locus_tag="PLM79_08205"
     CDS             15311..16795
                     /locus_tag="PLM79_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083764164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16253..16255,aa:Sec)
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV86331.1"
                     /translation="MIEIRVGEFAEKLGVHRNTVRNWIRSGTLRAANSVAKRYFITEG
                     ELVRFCRLQGIPESVMAQLMGKEVTVSPGKEEIMVQQSLRVPEVKTLGSRANTLRTDP
                     QWADVCLTCGSCASACPISGVDGMDPRKIIRMVLLGRIQEVIDSDWPWKCTLCGKCEE
                     ACPMNVELLKLFRKIRWLRDRDRVPGPLHKGVLMALRRGNNLGIPRSEFVGLLEEVGE
                     ELAEECCPGFRTPVDVEGADLLLTINSKEPFGEPDDMKFWWKIFYAAGESWTIPSENW
                     EGVNWGLFTGDDDAVRIMVGRIVDNVHRLGCKKLVLPEUGHAYYATRFGLEKWFKDDL
                     KRFEIVTTFDLLTDYLRSGKIRVDKSVHPQLTTYHDPCNYGRKSLKAFGRGYFEEPRW
                     IVGQCCENFVEMYPNREANYCCGGGGGAWALPFREERVFYGRIKQRQIRNTKAELVVT
                     SCHNCRDQILKSLRKEYNLDVEVKYIWELVSDALVLEPKQGDEG"
     gene            16818..19868
                     /locus_tag="PLM79_08210"
     CDS             16818..19868
                     /locus_tag="PLM79_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:16995..16997,aa:Sec)
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86332.1"
                     /translation="MTDQINGSIVVVGGGIAGMQAALDAANSGFYVYLLERQSAIGGA
                     MAKLDKTFPTNDCAMUIISPKLVEVGRHLNIELLPLTEVVSVSGEPGNFRVEILEHPR
                     YVDVEACIACGTCAEKCPRKVGDEYNEKLAQRKAIYIPYPQAVPQKYAIDRDHCIYFE
                     KGKCRACEKFCPTQAIRLDDRERERTLNVGAVILAPGFAPFDPKGIRTWGYGMFPNVI
                     TSLELERLLSASGPTQGHLVRPSDHKDVSRMAFLQCIGSRDLNKASNGYCSSVCCTYA
                     IKEAMIAKDHVKDLEVSIFFMDMRTHGKEFERYYERAKDMGIRFRRCRVHSLEPVPNS
                     NNVYFRYIDHRGKQIEDEYDLVVLSVGLCTPQGALDLARRADITLNANGFAENSSFNP
                     IATTRPGVFACGAFVGPKDIPQTVMEASAASAEATRSLAAVRNTLTRSKEYPAERPVE
                     GQAPRIGVFICHCGTNIAGVVDVKEVEDFAGSLPYVAHVERNLFSCSQDTQNIIRDRI
                     REKELNRVVVAACTPRTHEPVFRETLKAAGLNEYLFEMANIRNQNSWVHSNEPEKATQ
                     KAKDLVRMAVSKVGLLSPLPEMVVSVNQSALVIGGGLAGMAAALEMADHGFPVHLVER
                     SNLLGGQARFLEKTWKGEPVEPALQEMDARVRSHPLITLHMESVVTAAEGFVGNFRST
                     ISNGKGGETIEHGVAVIATGGQAHIPEEYSYGVSDRVFTAIEFDKLHRMGDRRLSEAK
                     TYVFIQCVGSREADRMYCSRVCCTHSVQAAIDLKEENPDREVFILYRDLRTYGEREEL
                     FRQARSKGVLFFNYEMHEKPRVNPVDPRTLEVFVWDHVTHEPLRIVTDIVVLATAIVP
                     NPANEELAKIYKLSTDTDGFLQEAHAKLRPVDFAADGLFLAGLAHYPKPVDEAVTQAK
                     AAAGRAMAVLANERYNLEPIKARVVPENCDGCALCLDVCPFKAISLVHEVGEAGRRVS
                     RIEVNAAQCKGCGMCQGTCPKDGVRVAGFSLDQINSQVEAALAS"
     gene            20041..20478
                     /locus_tag="PLM79_08215"
     CDS             20041..20478
                     /locus_tag="PLM79_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:20242..20244,aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV86333.1"
                     /translation="MEMDFEPKIIGFTCNWCTYAAADLAGVSRMQYPPSIRLVRVMCS
                     GMVHSKMVMNAFDLGADGVMVMGUHLGECHYQDGNHKAQARARVIEEMVRAYGLEKER
                     FQLVWCSSAEADRFVEAVTQMTDRIRQLGPSPLNRRVAQAAAS"
     gene            20490..21428
                     /locus_tag="PLM79_08220"
     CDS             20490..21428
                     /locus_tag="PLM79_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl viologen-reducing hydrogenase"
                     /protein_id="HOV86334.1"
                     /translation="MSVTVVQEWLNACSGCEISIVDMGERLLDVLGLVEFVHSPVLMD
                     HKYFGQLGDKTHLEIPEATVAIVSGGLRNEEHLEVLEEVRKKCAVLVALGTCATHGGI
                     PALANRHPMADVLKRYYETETTDAPLRYPDDGLPPLLDRCYALDEKVKVDVYLPGCPP
                     HPDQVFAALVALVQGTPLTLPAKSVCDHCPTVREGKGEVKKLRRFLQSPQYKGPDEPL
                     DKMRCLLEQGILCMGPVTRGGCGGDQNTPRCMTARVPCRGCYGPVKHTGDQRVEMLNA
                     LASNGIDLSALPEFASMLRFSGAHGLLKTIPKRQEA"
     gene            21433..22782
                     /locus_tag="PLM79_08225"
     CDS             21433..22782
                     /locus_tag="PLM79_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOV86335.1"
                     /translation="MAQVINIQPITRIEGHASVSIQLDDRGEVLDSKVRFMSLRGFEK
                     FIEGMPAEEVPRVVTRICGICPWQHHMASNKAVDRCYGVDLPPAGRKLRELMQTISHA
                     EDKILHFYFLAAPDFVMGPDSHFSVRNVIGIAKAHPELAAQVVRMRYQTKMILEKFAG
                     KAIHPHAGVVGGFSKPLLEEDRKEFLGKMKEMLDFACFTLDFAKTQVFPKYLDAVTTL
                     GVIETGFIGTVDDAGALNFYDGKIRLMKPDGSFTDFESRDYADYLGEHVEPFSYGKFP
                     YAKCWDEGFSLDVHNPRGIYRANTLARINVADRMATPRAQAELEEFREKFGRPAQLTL
                     LHHWARLIEEVYACEHAVELLEGPEICDPNVRAKVQPRAGRGVGCVEAPRGTLIHDYT
                     TDDNGCIVKANLIVGTTHNLGPINLSVGQAARALIHDGVVDETILNKIEMAVRAYDP"
     gene            22957..23202
                     /locus_tag="PLM79_08230"
     CDS             22957..23202
                     /locus_tag="PLM79_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86336.1"
                     /translation="MTEKKDKKRVVKVESADTACGGVPHMTVDQIAERLNIASKELCC
                     PACGRIHLTEEDVRKAEAKKYSETKRFRQIKEEAEGE"
     gene            23850..24545
                     /locus_tag="PLM79_08235"
     CDS             23850..24545
                     /locus_tag="PLM79_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOV86337.1"
                     /translation="MLVRDWMSTKVITVEAGAPLSEALDLLENYHIGMLPVLREGKLV
                     GMVTDYDLRPFASRVPSVTMGLDYSLVLSRVKVEDVMSRSHASVPVDYTIEEVADVLL
                     RNEIPGAVVVDAAHKIVGVITQTDISRVLVSVTGLWRGGIVYGFLLEDRAGSIKELTD
                     IVRAYGGRLASILTSYERAPRGYRAVHIRVRKLDRNRLQELENELKEKATILYKVDQR
                     LNTREIFENTQEK"
     gene            25357..>25794
                     /locus_tag="PLM79_08240"
     CDS             25357..>25794
                     /locus_tag="PLM79_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86338.1"
                     /translation="MKKSLYKTRWFWMSLLYLLVLPPASWAADDWDFLDRVPGDLRST
                     VGPVPSPGLRYPSLAAGEAEGIPIRVIKGEEGSLQVRVGMESKPNVDNRLLEIELFRI
                     ERIRGTDSRGIEHEFLDVLLPPTGTSTAEDGSMVFDPGTVAAGT"
BASE COUNT         5057 a         7836 c         8124 g         4777 t
ORIGIN      
        1 cgatccgggg tcaagacggc gaacaggacc cggtccatgc cgattccccg gtaaatccct
       61 tccaggagca ccgagaagag aaggttcaca tcgatgcggg tgtcctgcat cagggtcgag
      121 aggtcccgca gaacgttgaa ctgaaggacg ggatcgggta tcggaaaggc gggggaggaa
      181 accggggact cggattccgg ggagaactcg tccggggaag tggtccccga ggggaccagc
      241 ccggcttctc tgggcagggg aatcagccgg gaggatgcgg ccatgccgta gcttgcggcg
      301 atctcgaagg cttcgtgggc cgtttcgtgg agaagaggaa cgacttcggc ctcgggaagg
      361 ttcagaaact cggccacgtt ccggatgacc gccgcggctt cgggagtatc ccagccgtgc
      421 tggacattgt gggcgatcct atagcccaaa tccacggccc ggactcggat atccgcggta
      481 tccttccttt cgagagcggc atccaacagg tcgctaaggt gccattcttt gctcagacgc
      541 accgtgagct gctgcaggcg aaagcccaag acctcctgct cgatccgggc cgggggggtc
      601 ccggcgtcca gcttcagtgc gtcttccagg cgattggcca ggtctcggcc gaaacaccaa
      661 aaggcgatgg ggccgatgcg atagaggagg gcggcaatga agacctcttc ggtgtacttc
      721 tcgttgaagc gggagatgag cttcctggct tgggccgccg agtgaaaggc cgtggccatt
      781 tcgcggacga cctggtcgcg cagggagccg ttcaggaggg tctcgatcag gctgatggac
      841 aggcagaggc tgcgcacggt gtcgtacccc agcatgacca ccgcccggct gatggtgttg
      901 atgcgtcgga atcccttgtt gaaatggacg gaattggcga gtttgagcat tcgggccgtg
      961 agggcggcgt cctgcaaaac cacatgggcc agttccgaaa gggagctgtc gcgcctttcg
     1021 ctcaacccct ggatgttctt catgctctgg gcgaatatgg gcatatcttg ccgggtcaac
     1081 cggtggacga gtgcatccag ggcgctctgg gtcatggggt cctctctcac gggaggaaag
     1141 gcggtttcgt tagcgatgaa ggggttcgcg ggggagccct cggcgatccc cctcgaatca
     1201 gtactcgtgt tgcatggtgc cctaccttga gcagcagttc gaattcgatt ccggattcac
     1261 ccggcatcct agcacaaacc cccgacgttt gcacccgcgt gttccggttc gacgggagga
     1321 ttttccgggg cggattgcgg acccatgtcc ggaaaagagg gggaaagggg ccggtatctt
     1381 ggaatccttt tccccgatgg tggttcgacc atggacggcg gtgtctacaa acgcctcagc
     1441 cggcagcgga ggaacacctt cgagatgagg agggtatctt cgatgaggcg gaaatgggat
     1501 gagcggtttc cttcggggta gatcgtatgg atgggcaccg ggcggacttc caggtggagc
     1561 cgggtgaggg ttgcgagcac ctcgagctca taatcatacc tggacgaagg tagactcaag
     1621 attgccggga ccacgctcat ggggattccg cgcaatcccg tctgagtgtc ctccagggat
     1681 tgccccgtga ttcgcttcca gagccggcgg ctcatcgagt tgcccgttcg gctccgaaag
     1741 gggacctttc gggaaaagtc cctcaccccc agaacgagga ttgccggatg agattcgagg
     1801 gtcctcccga cgtgcaaaat gtcctgcaac cggtgctggc cgtcggcatc cgcggtggtg
     1861 actcccacgg cccggggaaa gtgatccaag gcataacgaa acccggtctt gagcgcggca
     1921 cctttgcctt ggttccggga atggcgcaag acggtcacgt tgcacacggt ctccaaggtc
     1981 cggaacaggt cggtaaactc cgcaccgctc ccgtcgtcca ccacaatgat cgaatgggag
     2041 gagtccgccc ttgcggttcc caccaccaga tcaagaagaa cgggaggagg gcggtaggcg
     2101 gggaacagga aaaccagcga cgggatcact ctttcctccc cgagcagatc ggtccggggt
     2161 cgaatggagg cccctgtcta agccgaacgg gttgtgaatg agcgcaggtg gatttcatcg
     2221 acccgtcgag tcaaggaccg ggacgcggtc ttttcatggg ggctcccgtt gcggccggag
     2281 caaggagtct gtcgaaacgg gggagaaatc ccggcccggg agccggacgg acctcgatcc
     2341 cggtgcccgg agggctaaac ggctttcaag cccgcgatct tgacggcgcc ccggtgataa
     2401 ccgtcgggaa acagtttggc cagttcgtcg atttcgacct ggtttgcggc acaagtctcg
     2461 tagaccgtcg gaacgaaccc cgtttgccga aactgctccc gcaggaaccg gataatccgc
     2521 cagtgcttct cggtgagttc cttggtcatt tttagttccc gcgccttgta gacggcgaac
     2581 tcttcgtccc actccgcagg atccaccagg aagcctctca cgttgacccg gtacaccttg
     2641 tcttcggaag gggacggcac ggtttccccg ttgtgccggg cggcccaggc ggggtggact
     2701 tgctcggccc ggtaggaaag gcctgcaagc ttgcaggcgc cgcgcaggta tccggaagga
     2761 aaaagccggt tcaggtccgc ggacttgagc ttgtgggtct tgcaggtttc gtggaccatg
     2821 ggacacttgc cggacttgtt gaaaatgcga cgaatgaagc ggatgatctg ccaatgacga
     2881 tcggacaggg gaagctccat ccccagcctc tccgccatgc cttcggcgaa gttctcgttc
     2941 cagtccgggg ggtgcagcag gaaatcctcg tcatccacct cgtacttctg tccgcgatac
     3001 tcaaaaatac gcatgttcca ttcctcccta ccacgattcc ggacggatcc ccgaaacaac
     3061 aacccccccc gatgtcgacg agactcgtcc tcatccttgg aaacggcggg tcgcgagggg
     3121 gcttccccga cacccgcacc tgccgccggc tcatcgtcac ggctcccatc actaagagaa
     3181 gatccttcaa cttgtcaaca gtttctgtat agtagcggtc cagccgtttg gaaaagaagc
     3241 cgaaggaact tgtggagcaa aagggggagc cggtgccgac catcgaggac gtgaaacgct
     3301 atctccggga tcaaggcatc gaggtgtggg agtttcggga ggccactccc acctcggagg
     3361 ctgcggccgg ggccgtaggc tgttgcgtgg gggaaattgc caaaagcgtc ctgttcctgg
     3421 tgggcggcga gccggtgctg gtggtgacct cgggggacgt gcgggtcaag tcctcccgcc
     3481 tcaagcaggc agtcggtctt gccgggcggg tccggttccc gaatgccgag gaagtggagc
     3541 ggtattccgg gtatttgccc ggcggagtct gcccgtttct acttcccgca aatctcaggg
     3601 tcctggtgga ttcctccctg cgtcgattcc cccgggttta cccagcggcg ggaaacgacc
     3661 attccgctgt ccccatttcc gtggatcggc tcctggaatt gacggggggc gaggaagcgg
     3721 acgtctgtga ggccgccccg ggggactccc gccgcccatg aggcccggcg ggctaccggg
     3781 aagattccag gatggaaagc aaatcggctt ccatgctcct gcgggccgcc ccctcgatcc
     3841 ttcccacttc cttcccgccc cggaccagca ccacggtggg cagttcccga atgccgtacc
     3901 gggcggcgac cccttcctcg tcccgcaggg agcggtccag ggcgtaaacg gcaagggcga
     3961 acgccgggtt gttcgtcttg tcgtacacgc gaagcagctt gggggcctcc atcaggcaat
     4021 cctcggacca ggtgcccaga aagagcagaa tatcggtttg gacggtgatc ctgcgaagct
     4081 tttccaggac ctttgccgcg gggacgtatt cttcggcatt gaggctgaag agcaggttgt
     4141 ccgtgatcag ttcttcccgg gagatccggc ctaccagcag gtcctccccg tcccttgtga
     4201 caatatgggg gttatcctgg cagaaaccga cgtcggccgg agccgacagg cagacctcca
     4261 cggcgaaaat gaaaagaagg agcacgacgg gggagagtct cttgggtttc atgggtccaa
     4321 ttcctttcgg gagggagtcg aggatttcgc ggcgcagact agcaccattc ccgtctacat
     4381 tcaaacccga gatgcacgca atgtcggccg gggttgacag gaacggaaaa gaaaggtaac
     4441 caggaagcaa gggttcggaa gcttcgcggc ttccgaaggt ggagatcgat agtcgggccg
     4501 ctatcgccgg acaggggagg gaaggcggcc gttcgtttcg aggggatacc ccataccgaa
     4561 tctttctgcg atttggcgat caaacacgcc gtccaaaaca ctgcctcgag ttctcgtcag
     4621 aagcattgcc tttcggcacc gtaccggtac cggctactgc ctgagatggt cgtgatcgaa
     4681 gtctcaccgt ccttttcctc gcccggacag ggggtcccgt tggggcttga gaatgcacat
     4741 cggcgccagg atcgttgaag aagacacagg ggatttcacg gtgtgggctc ccacgctcga
     4801 ttccgtcgag ctcgtcctcc tctccccccg ggcgggggtt ttccccatgg aaaaaggaga
     4861 gggggggtac tggaggtgcc gggtgccggg cattggggcg gggacccgct accggtaccg
     4921 actggaccgg gacgtccagc gacccgaccc ggcgtcccat tgccagtccg aaggggtcca
     4981 tggcccctcc cgggtcgtcg accacggagc ctttgcctgg gacgacgggc aatggcgggg
     5041 aattcccccc gaaaggatgg tcttctacga aatccacgtg ggcaccttct cgaaggcggg
     5101 cacctttgcc gggctcctgg agcggctgga cgagttggcg gacttgggcg tcaacgggct
     5161 gagcctcatg cccgtagccc agtttcccgg cgagaggaac tggggctacg acggggtgta
     5221 tcccttcgcc gtccagggtt cctacggagg gccggaggga ttgaaagagc tggtgaacgc
     5281 ctgccatcag cgcaacatgg ccgtcttcct ggacgtggtc tacaatcacc tggggcccga
     5341 ggggaattac ctgggacagt tcggacccta cttcacttcc cggtaccgga ccccctgggg
     5401 ggacgccgtc aactacgacg ggccctacag cgacggggtc cgaaactact tcattcaaaa
     5461 cgcccttcac tggtttggca attatcacgt ggacgggctg cgcctggatg cggtgcacgc
     5521 catttgcgac ctaagcgccc ggccctttct ccggcagttg gccgaggccg tggaggaatt
     5581 ctcccggcaa caggggagga agttttctct cgttgccgaa agcgacctca acgacgcaag
     5641 cctcatccgg ccccgggcct tgggggggcg ggggctggac ggaatctggt gcgacgattt
     5701 tcaccatgcc ttgcacagtc tcctgacggg agaaagggac gggtattacg aagatttcgg
     5761 aaagaccgaa cacctggccg gggcctaccg ggaagggttc gtgtactcgg ggcaatactc
     5821 ccggcatcgg aaaaggtccc atggaagctc ttgtcggcag tgcagtcctt ctcagctggt
     5881 ggtctttgcc cagaaccacg accaggtagg caaccgggtc cagggggagc ggatgtccgc
     5941 cctggtttcc cgggaagcct tgaagctggc tgcaggagcc tacctgctat cgcccttcgt
     6001 tccgctgatc ttcatgggag aggaatacgg ggaggaggct cccttcctct acttcgtgga
     6061 tcattccgac ccggacctca tcgaggcggt tcgccgggga cgccgggaag agtttcgaga
     6121 cttccatagg gaacagcccc cgccggaccc ccaatccccg agcacctttc tgcgatccca
     6181 actttgttgg gaaaagaggc gggaaggccc ccatggggtc ctcctccagt ggtatcggcg
     6241 gctcattgcc ctgagaaagc agattccccc tccggcttcc ttctccccgg aacactggcg
     6301 ggtggagtgg gaggaaaggg gccgaagcat ctggatccat cgccgagagg gagaccggga
     6361 acggctcctg gcgatgaatt tcggcacgga accctttgcc ctgccgtttc cttttcgggg
     6421 tcgctggacc aaggtcctgg attcatcgga agagtgctgg atggggccgg gttcggatct
     6481 tccctcctct cctcgatccg gtcaaaccgt gtgcctgagg ccgtttgccc ttgccttgta
     6541 tgagaaaacc gacggaaggg atttcccatg atgcgatacg tctgcttcca cggacatttc
     6601 taccaacccc cccgggagaa tccctggctc gaggacgtgg agctccagga ctccgcttac
     6661 ccgtatcacg actggaacga gagaatcacg gcggaatgct acgctcccaa taccgcctcg
     6721 aggatcctgg acggggagcg gaggatcgtc gacatcgtca acaactactc gaagatcagc
     6781 ttcaacttcg ggccgaccct tcttgcctgg atggaacggc accaacccga ggtgtatcag
     6841 gcgatcctcg atgcggatcg cctcagccgg gaacggtttt ccgggcacgg gagtgccgtt
     6901 gcccaggtct acaatcacat gatcatgccc ctggctaccc tccgggacaa atacacccac
     6961 tgccactggg ccgttcggga cttccaacgg cggttcggcc gcgatccgga ggggatgtgg
     7021 cttcctgaaa cggccgtgga caaagaaagc ctcgaggtcc tggcggacct gggaatctct
     7081 ttcaccattc tggcccccca tcaggccggc ctggttcgcc tcctcgggga aagcaactgg
     7141 agggatgtgg ggggagggcg catcgacccg accatggcgt acctttgcct gctcccctcg
     7201 gggcgaaaaa tccacctgtt cttctacgac ggtcccatcg cccgggaggt ggctttcggg
     7261 gggctcttga acaacggaga agcccttgcg gatcggatgc tgggagcctt ctcccccgag
     7321 cgggcacatc cccagatcgt gcatgtggcc acggacggag aaacctacgg gcatcatcat
     7381 cgcaaggggg acatggccct ggcctatgcc ctctatcaca tggaagccaa cgaaaccgtg
     7441 cgggtaacgg tctatggaga atacctcgaa cgacaccctc ccacccagga agtcgagatc
     7501 gtggaaaaca cctcgtggag ctgcatccac ggggtcgagc ggtggagggc ccattgcggg
     7561 tgctcgagcg ggtcccgccc cccatggaac cagcagtggc gcgcccccct ccgtcgagcc
     7621 atggatcagc ttcgggactg ggtgacgcct tatttcgaac gggaggcgtc caactacctg
     7681 gcggatccct ggaaggcgcg aaacgaatac atcgatgtgg ttgtggatcg ttcccgggag
     7741 agtgtcgagg cgtttttccg tcgacatgcc gtgggggaga tttccccgga aatccggact
     7801 cgtttgttga agcttctcga gatgcagcgg cataccatgc tcatgtacac cagttgcggg
     7861 tggttttttg acgagatctc ggggctcgaa gccacccagg tcataaaata tgccgcccgg
     7921 gccatccagc tggcccggga gaccctggga acggacttcg aggaatcctt cctggaaatc
     7981 ctcgaggagg cgccttccaa tatcccggag ctgggcaacg gcaggagggt ctacgaacgg
     8041 tacgtgaagc cttcgaggat cgatcttccc cgggtggcgg cccattacgc catcacctct
     8101 cttttcgagg aaacccagga aagcgccctc atcgactcct atcgggtgga acgtacctcc
     8161 cacgagagta tgcaggccgg ggtcctgaag ctcgaagcgg gccggttgga catccgatcg
     8221 gaggttaccc gggaagaaac ccgtcttttc tatgccgccc ttcacctggg gggacacaac
     8281 gtaacgtgcg gcgttctggg cgccgaggag gaaaaccgtt tccaccaaat gtcggaagag
     8341 ttcgaggctc ccttcgcccg ggcggacatt ccggagctgg tccgcctaat ggaccgaacc
     8401 tttcagggga acctctacac cctgtggcac ctgtttcggg accgccagca aaaggtcctg
     8461 gagcaggtcc tggccccggg gctcgaggag gcggagttcc tctttgccca aatggtggaa
     8521 aggaactacc ccctcatgaa tttcctccag gagctccaga gacctcttcc ccggcacctc
     8581 gaggtggccg ccgagttcct ggtgaacacg gaccttcgca agatcttcca aagcaaggag
     8641 ccggacctgg atctcctgga gcgtcgaatc catgaagccc gaagatggtc cgtgcacttg
     8701 gacagcaacg ccctggggtt cggcgtggaa atgaaactga gccggatcat ggcggagttc
     8761 gccgaggacc ccgccgacct ggaaagactg gaaaggatcg aacggctctt tcggctttta
     8821 aatgaatcgg gcctggacct ggacctgtgg gaggcccaga atcgctattt cgccgtggct
     8881 cgaagcgcct tccccgaggt ccggggtcga gccggggaag gggatgcccg ggcagccgaa
     8941 tggacccggg tttttctcga tctgggggat cacctgggcg tgggggtccc atgattcgca
     9001 ccccgctggc cacctaccgc attcagatga accgggaatt cgggttccag gccttgaacg
     9061 aaatcctggc ctacctggcc gccctgggga tcagcgacct gtacctatct cccatcctcc
     9121 gggccagaaa ggggagcgct cacggctacg acctggtgga gccccgggaa ttgaacccgg
     9181 aactggggag cgagaaggat ttccggcgcc ttgtggaagg agtgaaggag cacggactgg
     9241 gatggatcca ggacatcgtt cccaaccaca tggccttcga tccggacaat tccatgctca
     9301 tggacgtcct cgaaaaccgc gaaaactcca actactatcc cttcttcgat atcgattgga
     9361 accatcccca cgacgatctc aaggaccacg tcctcgcccc tttcctggga agattctacg
     9421 gggaatgcct cgatgagggg gaaatccggg tgtcctacgg acccgacggc ttcaaggtcc
     9481 actactatga tctccgattt cccctgaaag tggaatccta cagcgaggtg ttgaccccga
     9541 acctgggaac cctgaaaaaa gacctgggag aagaagaccc cgatttcatc aagctcctgg
     9601 ggacccttta tgtgctcaaa accttgcccg gccgggaagc ccgggacgag cgctacgggc
     9661 agatccggtt catcaagcgg gttttgtggg agctccacac gaccaatctt cggattcgag
     9721 agcacgtgga cggaaacctg gagcgcctca acggccggga aggcggaccc aaccgtttcg
     9781 cggccctgga gggtcttttg gcccaacaac actttcgcct ggccttctgg aaagtggcca
     9841 ccgaggagat caattaccgg cggtttttca atatcaacga tctcatctcc ctgcgcatgg
     9901 aagacaagaa ggtcttccag gagactcatg ccctggtgct gaagctggtg aaggagaagg
     9961 tgttcaccgg cctgagagtg gatcacatcg acggcctgta cgacccgacc cagtacctga
    10021 gccgcctgcg ccaggaggcc cgtgggacct acctggtggt ggaaaaggtc ctcgatcggg
    10081 acgaatccct gccggatttc tggccggtgc agggaacgac cggctacgat tttctcaacc
    10141 gggtcaacga gctgttttgc cgcagggaac acgaaggccg gttcggccga atctacgccc
    10201 gccacggagg gaccgggcaa cccttcgtgg atatggtcgc cgagaaaaaa cggctgttca
    10261 tcgcccgaca catgaccggg gacatcgacg gcctggccca cctgatcaaa tccatctcgg
    10321 ggcgcactcg gcagggaagc gacatcaccc tgtacggcct gagacgggcc atgacggagg
    10381 tgatgtccta cttcccggtc taccgcacct acctcagcgg taagaccctg cgggaggaag
    10441 acctggccgc catccggcag gcggcggccc aagcggcgga tcgtgttccg gccctgacct
    10501 acgagctttc tttcctccag gacctcctgt ctctgaatct ggacgggtcc ctccgggagg
    10561 aagacagaga ccggtgggtc cacgtgatca tgaggttcca gcagttcacc ggtcccctca
    10621 tggccaaggg gttcgaagac accaccctgt acgtctacaa ccggctcatt tccctgaacg
    10681 aggtgggggg aaatcctcaa cggttcgggg tgtccggcga cgagttccat gagtacatgg
    10741 gtctgcgggc ggcccgacag ccccagtcca tgaacgccac cgccacccac gacaccaagc
    10801 ggggagagga cgttcgagcg aggatccacg tgctctcgga gatccccggg gaatgggaac
    10861 tgtgcctgaa gaagtggagc caatggaacc gtcggaacaa gcgccgcctc gggggtgtgg
    10921 aggctccgga caaaaacgac gagtatttcc tctaccagac cctggtgggg gccgccccct
    10981 ttggaggacc ctgggggcgg gaattccggg accgaatgga agcctacgtc atcaaggccg
    11041 tgagggaggc caaggtccat accgcgtgga tcaagccgga cacggcctat gaagaggcct
    11101 acctcgactt tgttcggacc cttttgaccc cctctccccg gaatcccttc ctggaagact
    11161 tcccgccctt tcaaagaaaa atcgcgttct acggagtctt caactccctg gcccagaccc
    11221 tggtcaagat cacggccccc ggagtgccgg acttctacca gggcacggaa ctgtgggacc
    11281 tttccctggt ggaccccgat aaccgacgcc ccgtggactt tgccgccagg agctcctccc
    11341 tggccgacct ggaatccgcc tggaggagga accctgccca ccttcttgcc gacctgttga
    11401 cctgccgtgc ggacggaagg atcaagcagt tcgtcatctg gaggggactg caggatcgca
    11461 gggaacggcc ggacctttac cgcaagggag ggtacctgcc gttgagggtg gaaggcggcc
    11521 gaaaagacca tgttcttgcg tttgccagac aatacaggaa cgaatgggcc gttacggtgg
    11581 tgccccggtt cctgacctcg gtggtcgaag ccggccgaga tcccttgggc gatccggttt
    11641 ggcaggacac ccggatcctc cttccccaag aggctccggt ccgctggcgc aatgagttta
    11701 ccgcccagtc tctttacggg gagaataatg ccctgcccct gggaagagtg ctggagcgtt
    11761 ttcccgtggc ccttctcggc caatggcgga gaaaggcccg gacagtttcc aaccggacat
    11821 aaggatggac catggccgat ccctttgccg tcaaggactg tgctctcatt gccattgcca
    11881 cgggagagca tgcccagaac ctgcgggaga tgcgagatca tctcctcacg gtgcaccccg
    11941 gttgcatcta tcaccacttt tggggtggga tgctgcaacc tcgcttcgat gagccggagt
    12001 acatgaacga tttcgccgcg tgggcctggc ggggccttca tgatcccggc cttgccgaac
    12061 gactggccct catcgatccc acccactacc gggacctcga agaactgcgc cgggaactgc
    12121 tggaggtcat cgaagagcgg ctggatgaaa cggaaatggt gccctggtcc cgcaccggcc
    12181 aggcgtttca tttcattcgt tcccggatcg tggtgttcga tacccgcatt cgcatttcgc
    12241 acccccggga actgagggat cgtatcgggc agctgcccct ggggagtgtt ttctaccact
    12301 tcgtggatgc ccggcgccga gcgcccatcg gaaagaacga tttcagtgcg tggttgatga
    12361 gccttgacgg ggattggtcg gaactggcgg agatccttgg gaacgtggat ccatacttca
    12421 cgacccttgc cgaattgcgg cgggaattcg aggaagtcct aaaagcctat gcagtgtgag
    12481 gggattcaag tgaacggtct tttgcagaag tatgcccaga tcgtgggaga agaggtggtg
    12541 gatcacctcc ggcagctggc ggagccgctg agagggttga aggtcgtaca cgtgaattcg
    12601 actcgagagg gcggcggagt ggcggaaata ttgagctggc tcgttcccct caagaatgag
    12661 ctggggatcc ataccgaatg ggaggtcgtc accgggagcc aggagtttta ccagtgcacc
    12721 aagggctttc acaacgccct ccagggaaac cgggtcgaaa tccccggatc tctcctgcgg
    12781 gcttacgagg gagccaatga agaaaacgcg gatcgattga gatcgagcct cgaagaagcg
    12841 gacgtcgtct ttatccatga tccccagccg gctcccctcc tgcgcctttg ttccgggaga
    12901 aggggaaagt ggatctggcg ctgtcacatc gacctgagcc gcccgtatcg tcccgtctgg
    12961 aaatatctgc gcggctttgt ggaaggctat gacgccagcg tctggtccct ggcccagttt
    13021 gcccaacccc ttcctcaccc cctttacctc atcgccccca gcatcgaccc actcagcgag
    13081 aagaaccggg agctgacccc ggacgaggtc ctgggcgtag cggttcgatt cgggttggac
    13141 cccaaacgga ccgccatcac ccagatctcc cgcttcgacc ggttcaaaga cccggtgggg
    13201 gtgattcgag cctatcgcct gtgcaaagaa tacacgccgc tgcaactggt gctggcggga
    13261 gggggagcca cggacgaccc cgaaggggaa caggtgctcc gggaggtgcg aacggccgcg
    13321 ggggacgatc cccacattca tgtgcttctt ctgcccgcgg actcccaccg ggaaatcaac
    13381 gccctccagc gcttttccca gattgtggtg caaaagtcca cccgggaagg cttcggcctc
    13441 acggtgaccg aggccatgtg gaagggaaag ccggtcatcg gcggggacgt cgggggaatc
    13501 cgcctgcagg tggtcaatca ccacacgggc tttctggtca acagtcccga gggcgcggcc
    13561 ctgagaatcc ggtacctgtt gaaacatcgg gagcatatgc tcgagatggg aagaaaggcc
    13621 cggcagttcg tgcggaacaa ctttcttctc acccgccacc ttcgcgaata cctcaccctg
    13681 gtgcacgcca tccggcatga ggagcgggag aggatcgagc taaacggcct ccaatcggga
    13741 ctctagaggt ctaccctttc ccacatcttt gctactgcac gtgagacgtc tcctcacacg
    13801 aggaactcgg agggttcgcc gccgtcggcc ggcggtttcg ctcgtatggg cgacatcacc
    13861 gccccctggg gtcaggtctc cgtatggctt ttgccgtgca tggggagggt gtagggggcg
    13921 gccggccggt cgccccccag gcgtgtaggg cgcaatccca ctcttgtcca ggggacggtc
    13981 ctgcgacccc atgggtcgcc ttcctcttgg gacctgttcc gggggtatcg gccttgcggc
    14041 ctcgaccccc ggttcatggt tgcggacccg ccggggttcg ctcggagacg gcgatcgatc
    14101 cccgccgaat cgcagatgtg ggcaaaaggg cagcgatagg tcttccggtt gcggcttgcc
    14161 ggaagggtgg gtccccgaaa gccttcctac gggctgccgt gggtgaaatt tccggggccc
    14221 ccgtcccggt cccgaaccct cggttcatgc gtcatcccgc ctcctcgtcg ccaaaccccg
    14281 cggggggacg gcaacgaggg agattgcctc ggggtctcca ggtcggggac cgacgggggc
    14341 cgcttttccc ggatcttctc ttggggccgg gcgtggtttc cgaccctcct tgcccggcga
    14401 gattcccttc ccgttttccc tcggttttct tgatttccgc ccccgcggtt cgggggcggg
    14461 cgcttttcgg gtttgatttc gaggctcccc ggccccctcg aggatcaaga aaacattgtt
    14521 ttaccttcct tttgtccttc cctcattttc tccttgacat gcccatccgc ttgtgtaaaa
    14581 tggcgcacat tgcacagttg gcatagatgg cacagcacga gtgcacaacg gcctgcgggc
    14641 cgaatccaag gccttctccc cccgggaaaa agaaccgggg gtatgaggat atgggcgtaa
    14701 cggaagagaa accaactctt ggacggaggg ggatttggag acccgaaacc cgggtcccaa
    14761 ccaggctcta agaaaattgc ataggccctc ttgatcccat cccgctcgtc ttcctccccg
    14821 gcttccgctt ctccttcgct tctccttggc ttccccggac ccggcattcg atcccgactt
    14881 tccttcggcg cgcttcctcc cggtctcccc ggaccgaccc acgggaacgg ctccccgcac
    14941 gttctcggat cccggcaacg ggaacccgtc tcccccgttt tgcgcccggt gaacacacgg
    15001 ccaaaagccg cctgaggggt cttcccgggg caggcattcc cggaaggtcc ccgcccatcc
    15061 ttcaccctat ccgggtgaaa gtcttgtccg aaccttgtct gatcccaaac ggaccgtcgg
    15121 ggaccggggt gaggaaccca tccgccccgg aggctcaaac cgccgttccc catgggcgat
    15181 cgttcgtctt cggcagcggg atccgggcat cgtgcttcgc gggtgccctc gatgtcggag
    15241 caggacccgg ggttccggca gtcggggagt tccccgcgga gggctttacg aacttcaatg
    15301 ggagatgacg atgatcgaaa ttcgggtggg agaattcgcg gaaaagctgg gagtccaccg
    15361 caacacggtg cgcaattgga tccgtagcgg gaccctgagg gccgccaatt cggtggccaa
    15421 gcgttatttc attaccgagg gggaactcgt ccgtttctgc aggctccagg gcattcccga
    15481 aagcgttatg gcccagctga tgggaaaaga ggtcaccgtt tccccgggaa aggaggaaat
    15541 catggtccag cagtcgttgc gggtcccgga ggtgaaaacc ctgggatcga gagcaaacac
    15601 cctgcgaacg gatccgcagt gggcggacgt ctgcctcact tgcggcagtt gcgccagtgc
    15661 gtgtcccata tcgggggtgg atggaatgga tccccgaaag atcatccgga tggtcctttt
    15721 gggtcgaatc caggaagtga tcgattcgga ctggccctgg aagtgcaccc tgtgcgggaa
    15781 gtgcgaagaa gcctgtccga tgaatgtgga actcttgaag ctcttccgga aaatccggtg
    15841 gcttcgggac cgggaccggg tcccgggtcc cctgcacaaa ggggtgctca tggccctgcg
    15901 ccggggcaac aacctgggaa tcccccgctc ggagttcgtg ggtttgctcg aagaggtggg
    15961 ggaggaactg gccgaggaat gctgtcccgg tttccggacc cccgtggacg tggaaggggc
    16021 cgatctcctt ctgaccatca attccaagga gccattcggg gaacccgacg acatgaagtt
    16081 ctggtggaag atcttctatg ccgccgggga atcctggacc attccgtcgg aaaactggga
    16141 aggggtcaac tggggacttt tcaccggcga cgacgatgcc gtgagaatca tggtgggccg
    16201 gatcgtggac aacgtccacc ggctgggctg caagaagctc gtcttgcccg agtgaggcca
    16261 cgcctactat gcaacccggt tcgggttgga aaaatggttc aaggacgacc tcaaacggtt
    16321 cgaaatcgtc accaccttcg acctgctcac ggactatctg cgaagcggca aaatccgggt
    16381 ggataagtcc gtgcatcccc agctcaccac ctatcacgac ccctgcaact acggcagaaa
    16441 gtccctgaag gccttcggga ggggctattt cgaggagccc cgctggatcg tggggcaatg
    16501 ctgcgaaaat ttcgtggaaa tgtatcccaa ccgggaggcc aattattgct gcggaggggg
    16561 tggaggagca tgggccctgc ccttccggga ggagcgggtc ttttacggaa ggatcaagca
    16621 gcgccagatc cgcaacacca aggcggagtt ggtggtcact tcctgtcaca actgtcggga
    16681 ccaaatcctc aagagtctgc gcaaggaata caacttggac gtggaggtca agtacatctg
    16741 ggaactggtc tccgacgcct tggtcctcga gccgaaacag ggcgacgagg ggtaaccgtc
    16801 ccagagagga ggcaaacatg acggatcaga tcaacggctc catcgtcgtg gtcggaggag
    16861 gcatcgcggg gatgcaggcg gccctggatg cggccaactc cggcttctac gtctacctgc
    16921 tggaaaggca gtcggccatc ggaggggcca tggccaagct ggacaagacc tttcccacca
    16981 acgactgtgc catgtgaatc atctctccca agctggtcga ggtcggccgg catctgaaca
    17041 tcgagcttct tcccctcacc gaagtcgtct cggtctccgg ggaacccggc aatttccggg
    17101 tggaaatcct cgaacacccg cgctacgtgg acgtggaagc ctgcattgcg tgcgggacct
    17161 gtgccgaaaa gtgcccccgg aaggtcgggg acgaatacaa cgaaaagctc gcccagagaa
    17221 aggcgatcta tatcccctat ccccaagcgg tgccccagaa atacgccatc gatcgggacc
    17281 actgcatcta cttcgagaag ggcaagtgca gggcctgtga aaagttctgt cccacccagg
    17341 ccatccggtt ggacgaccgg gaacgggaac gaaccctgaa cgtcggggcc gtgatcctgg
    17401 ctcccggctt cgctcccttt gatcccaagg gcatccgcac ctggggctac gggatgtttc
    17461 ccaacgtcat cacgtccctc gagctcgaac ggctcctttc ggcctcgggt cccacccagg
    17521 gacacctggt tcggccttcg gatcacaagg acgtgagccg catggcgttt ctccagtgca
    17581 tcgggtcccg ggacctgaac aaggcttcca acgggtattg ctcgtcggtt tgctgcacct
    17641 acgccatcaa ggaagccatg atcgccaagg accacgtcaa ggacctcgaa gtgtccattt
    17701 tcttcatgga catgaggacc cacggcaagg agttcgagcg ttactacgaa cgggccaagg
    17761 acatgggaat ccggttccgc cgatgccggg tccattccct cgaacccgtt cccaactcca
    17821 acaacgtcta cttccgctac atcgatcatc ggggaaagca gatcgaggac gaatacgacc
    17881 tggtggtcct gtccgtgggc ctgtgcaccc cgcagggggc tctggacctg gcccggcggg
    17941 cggacatcac cctcaatgcc aacgggtttg ccgaaaactc ctccttcaac cccattgcca
    18001 ccactcgccc cggggttttc gcatgcgggg cgtttgtggg acccaaggac attccccaga
    18061 cggtcatgga agcctccgcc gcctcggccg aagccacccg atccctggcc gccgttcgca
    18121 acaccctcac ccgaagcaag gaatatcccg ccgaacgccc cgtcgagggg caagctcccc
    18181 gcatcggggt cttcatctgc cattgcggca ccaacatcgc cggggtggtg gacgtgaagg
    18241 aggtggagga ttttgccgga tcgctgccct acgtggccca cgtggaacga aacctgttca
    18301 gctgttccca ggatacccag aacatcatcc gggatcgaat ccgggaaaag gaactcaacc
    18361 gggtggtggt ggccgcctgc accccccgca ctcatgaacc cgtcttccgg gagaccctca
    18421 aggcggcggg gttgaacgaa tacctgttcg agatggccaa catccgcaac cagaattcct
    18481 gggtgcattc caacgagccg gaaaaggcga ctcaaaaagc caaggacctg gtgcgcatgg
    18541 ccgtgtccaa agtcgggctg ctcagccccc ttcccgagat ggtggtcagc gtcaaccagt
    18601 cggccctggt gatcggaggc ggcctggcag gcatggcggc ggccctcgag atggcggacc
    18661 acggcttccc cgtccacctg gtggagagaa gcaacctgct gggaggccag gcccggttcc
    18721 tcgagaagac ctggaaggga gaacccgtcg agcccgccct tcaggaaatg gatgcccggg
    18781 tacgcagcca tcccctcatc accctgcaca tggaatccgt ggtgacggcc gccgaaggct
    18841 ttgtggggaa tttccgatcc accatctcca acggcaaggg aggcgaaacc atcgagcacg
    18901 gggtcgccgt catcgcaacg ggcggtcagg cccatattcc cgaagagtat tcctacgggg
    18961 tatcggaccg ggtctttacg gccatcgagt tcgacaagct ccaccggatg ggagatcgac
    19021 gcctgtccga ggccaagacc tatgtcttca tccagtgcgt cgggtcccgg gaagccgacc
    19081 ggatgtattg ctcccgggtc tgctgcaccc attcggtgca ggcggccatc gacctcaagg
    19141 aggaaaaccc cgaccgggaa gtgttcatcc tctatcgcga cctgaggacc tacggggaaa
    19201 gggaggaact cttccgccag gcccgttcca agggagtcct cttcttcaac tatgaaatgc
    19261 acgaaaaacc cagggtgaac cccgtggatc ctcggactct cgaggtcttc gtgtgggatc
    19321 acgtgaccca cgagcccctg cgcatcgtga cggacatcgt ggtcctggcc acggccatcg
    19381 tgccgaaccc cgccaatgaa gagctggcaa agatctacaa gctctccacc gacaccgacg
    19441 gctttctcca ggaggcccat gccaagcttc gccccgtgga ttttgccgcc gacggactgt
    19501 tcctggccgg gctggcccat tatcccaaac ccgtggacga agccgtcacc caggccaagg
    19561 ccgccgctgg gagagccatg gcggtcctcg ccaacgaacg ctacaacctc gagcccatca
    19621 aggcccgggt cgtgccggaa aactgcgacg gctgcgccct ttgcctggat gtttgtcctt
    19681 tcaaggccat cagcctggtg cacgaggtcg gggaggcagg acggcgcgtt tcccgcatcg
    19741 aggtcaacgc ggcccagtgc aaggggtgcg gaatgtgcca ggggacctgc cccaaggacg
    19801 gagtgcgggt ggcggggttc tccctggatc agatcaattc ccaggtggag gcggccctgg
    19861 cgtcctgaaa agggcgaggg gcgaggggcg agaggctgga ggcgaggcgc gaatccccgg
    19921 aggcgggact ccccgaggcg ccccgcccgg gaaagccgtc ctcccccggc ccgatccgtc
    19981 gccccggacc caaggcgacc aaaggtcgaa tcgagagacc gacggtcttt cttcaaggag
    20041 atggaaatgg attttgaacc aaaaatcatc ggctttactt gcaattggtg tacctatgcg
    20101 gccgcggacc tggccggggt gtctcggatg caatatcccc cgagcattcg gctggtccga
    20161 gtcatgtgct cgggcatggt tcattccaag atggtcatga acgccttcga cctgggggcc
    20221 gacggcgtca tggtcatggg atgacacctg ggcgaatgtc attaccagga tggtaatcac
    20281 aaagcacagg caagagcccg ggtcatcgag gaaatggtgc gtgcgtacgg gttggagaag
    20341 gaacgattcc aactggtgtg gtgctcctcg gcggaagcgg atcgattcgt agaggccgtg
    20401 acgcaaatga cagatcggat tcgtcagctg ggaccgtccc ccctgaaccg ccgggtcgcc
    20461 caggctgccg cttcatgagg aggacagaca tgtcggtgac cgtcgttcaa gaatggctga
    20521 atgcatgttc cggctgtgaa atatccattg tggatatggg agaaaggctc ctggacgtgc
    20581 tgggtttggt ggaattcgtc cacagtcccg tgctcatgga tcacaagtac ttcgggcagc
    20641 tgggggacaa gacccatctc gagattcccg aggccaccgt ggccatcgtc agcggggggc
    20701 ttcgcaacga ggagcacctc gaggtcctgg aagaggtgag gaagaaatgc gcagtgctgg
    20761 tggccctggg gacctgtgca acccacgggg gaattcccgc cctggccaac cgccacccca
    20821 tggccgatgt cctcaagcgc tactatgaaa cggaaaccac ggatgccccc ctgcgctatc
    20881 ccgatgacgg acttccgcct ctcctggacc gatgttatgc cctggacgaa aaggtgaagg
    20941 tcgacgttta cctgccggga tgtcctcccc acccggacca ggtgtttgcc gccctggtgg
    21001 ccctggtgca gggcacccct ctgaccctgc ccgccaagag cgtttgcgat cactgtccca
    21061 cggtgcgcga gggcaagggg gaggtaaaaa agctgcggcg tttcctgcaa tcgccccagt
    21121 acaagggccc ggatgaaccc ctggataaga tgcgctgtct cctggaacag gggatcctgt
    21181 gcatggggcc ggtcacccgg ggaggatgcg ggggagacca aaacactccc cggtgcatga
    21241 cggcgcgggt cccctgccgg ggatgctacg gtcccgtgaa gcataccggg gatcagcgcg
    21301 tggagatgct caatgccctg gcatccaacg ggatcgatct ttcggccctt cccgaattcg
    21361 cctcgatgtt gaggttctcc ggcgcccacg gcctgctcaa aaccatccca aaacgtcagg
    21421 aggcgtaaga atatggcaca ggtcatcaac atacaaccca tcacgcgaat cgaggggcat
    21481 gcctccgtgt ccatccagct ggacgaccgg ggagaagttc tggacagcaa ggtccggttc
    21541 atgtcccttc gcggattcga gaagttcatc gaggggatgc ctgccgagga agttccccgc
    21601 gtggtgaccc gcatctgcgg gatctgcccc tggcagcacc acatggcttc caacaaggcc
    21661 gtggaccgct gctacggggt cgatcttccc ccggcgggca ggaaactgcg ggaactcatg
    21721 caaaccatct cccacgccga agacaagatc ctccacttct acttcctggc ggccccggat
    21781 ttcgtcatgg gaccggacag ccacttctcc gtgcgaaacg tcatcggcat tgccaaggcc
    21841 catccggaac tggccgccca ggtcgtccgg atgcgctacc agaccaagat gatcctcgaa
    21901 aaattcgccg gaaaggccat tcatccccac gccggggtgg tcgggggctt ttccaagccc
    21961 ctgctcgagg aggaccgcaa ggaattcctg ggcaagatga aggagatgct ggattttgcc
    22021 tgcttcaccc tggattttgc caagacccag gtcttcccca agtacctgga cgccgtcacc
    22081 accctgggag tcatcgaaac cgggttcatc ggcaccgtgg acgatgcagg ggcgttgaac
    22141 ttctacgacg gaaagattcg cctcatgaag cccgacgggt cctttacgga cttcgaatcc
    22201 cgggattatg ccgattacct gggggaacac gtggagccgt tttcctacgg caagttcccg
    22261 tacgccaagt gctgggacga gggattctcc ctggacgtcc acaatccccg ggggatctac
    22321 cgggccaata ccctggcccg catcaacgtg gcggatcgca tggcgacccc ccgcgcccaa
    22381 gcggagctcg aggagttccg ggaaaagttc ggcagaccgg cccagctcac cctgctgcac
    22441 cattgggccc ggctcatcga ggaggtgtac gcctgtgagc atgccgtgga actgctcgaa
    22501 ggtcccgaga tttgcgaccc caacgtgagg gccaaggtcc agcccagggc gggcagggga
    22561 gtgggatgcg tcgaggcgcc ccggggcacc ctcatccacg actacaccac cgacgacaac
    22621 gggtgcatcg tcaaggccaa cctcatcgtg gggaccaccc acaacctggg acccatcaac
    22681 ctgagcgtcg gccaggcggc ccgggccctc atccatgacg gggtcgtcga cgagacgatt
    22741 ctcaacaaga tcgagatggc cgtgcgcgcc tatgacccct gaatttcctg tgccacccat
    22801 cgcctggacg gcgatagcgg cgtatccatc gaagtcaggg attcccgggg tgaagtcctc
    22861 acgaaatggc cccccgtaag ggccgcccgc tgattgtccg ccgacggccg ttgcccgaag
    22921 cggagtgtta ggccccatgg gcacatggag ggactcatga cggaaaagaa agacaagaaa
    22981 cgggtagtca aggtcgagtc cgccgacacg gcatgcggag gcgtgcccca catgacggtg
    23041 gatcagattg ccgagaggct caatattgcg agcaaggaac tgtgctgtcc cgcctgcggg
    23101 cgaatccacc tcaccgaaga ggacgtcagg aaggccgagg ccaagaaata cagtgagacc
    23161 aaacggttcc ggcagatcaa ggaagaggcc gagggagaat gacagtcccc gaaatcagcg
    23221 gtttcatgcc gttggtttgc ctcagaggac gggcataaag accgcccgat acgggcggca
    23281 cggcatgccg cgcccttgcg gggacgggcc aaccggtccc gtcgaggcgg gggttctccc
    23341 cccgtcggga gtcccgctga atggggaacg accggcaagg atcgccggag gtcgagcttc
    23401 ccgagggagt cggggagaga cgctgagaag accagccccg ggagcggcct ctcggaccgg
    23461 gggggcagcc ttgaggtctt ccggcccggg gatggaaacc ccaactttcg gggcatgggc
    23521 cggggtttcc aaccgaccgg cgggagggtc gtcgccgaaa gtcctccccg ttccccggtg
    23581 aaccgaacct gctccccttc gcggatggga gagggcggga cgggggcagg cagcccattg
    23641 acagaggggc cctcggcctg gatggagcga ttcggcggct acggatcccg ttcgtcatgc
    23701 ccgcgcaggc gggacccgga aaaggcacgc atgcgctccg gatgcccgcc ctcccgggca
    23761 tgaccgccgg atgccctcgg aatcgctcga agtttggata tgatacagtc gcatcaggag
    23821 ccgtaaatgc ttgtgatgaa ggagatgcta tgctggttag agattggatg agtaccaagg
    23881 tgatcaccgt ggaggcgggt gcacccctct ccgaggccct ggacctcctc gagaattacc
    23941 acatcgggat gctgccggtg ctgcgggaag ggaagctggt tggaatggtg accgactacg
    24001 acttgagacc cttcgcctcc cgggttcctt ccgtcaccat ggggctggac tattcactgg
    24061 tcttgtcccg ggtgaaggtc gaggacgtca tgtcccggtc ccatgccagt gtgcctgtgg
    24121 actacaccat cgaagaggtg gccgacgtgc tgctgagaaa cgagattccc ggtgccgtgg
    24181 tggtggatgc cgcccataag atcgtggggg tcattaccca gacggacatc agtcgagtcc
    24241 tggtttccgt taccgggttg tggcgcgggg gaatcgtcta cgggttcctc ctcgaggacc
    24301 gggccgggtc catcaaggaa ctcaccgaca tcgtgcgggc ttacggaggc cgacttgcca
    24361 gcatcctgac gtcctacgaa cgggcgcctc ggggctaccg ggcggtgcat atccgcgtgc
    24421 ggaagctgga ccgcaaccgg ctgcaggagc tggaaaacga gctgaaagaa aaggcgacca
    24481 ttctctacaa ggtggatcag cggctgaaca cccgggagat tttcgagaac acccaggaaa
    24541 agtgaccgaa accctcgatt cgtcgttgcc gccgggcacc caggtgtggg ttcgggaggg
    24601 tcggcgcacc cgaacccggg cgatctcggg gcttctagaa gcgcgtgggg cgggcgcgga
    24661 gcccgcccct ccgtcgggct gccgatccgt cgtgtgcatg ttgtccacca gcagggtacc
    24721 gggaatctcg ttggaaaaga gggtgccccc ggggagaagg agtcgcaatg ccgggggccc
    24781 ggccggggcc cgggttaaca tgcgggtggg caatattcgc ggtttttgtg cccggtgccc
    24841 ggacccgctc ccggggtgtc aaacccgagg gaaccggatc cgtttccggg ccatcgaacc
    24901 cggcggggag ggaccgatcc gctgtgctga cccgacgcac ccggtctcgt ggcattctcc
    24961 cggccgatcc ccaacccttg acctcccggg tggagcgatt cttcgggcat ccgcctttcc
    25021 cacagtttcc tgatcggccg tataactcgc ctttgcaggc atttgggtct caccggaagt
    25081 ccttttcaag aaactcttcc ccccctgcat ccccctccag ggacgcgaac cgccccgccc
    25141 ggaaagcctc gccctagtcc cgttccgtgg tccgcagaaa gccccgctcc aacccggatc
    25201 gaatccgtag aacgaatctc cttgccggtc tacgagagct gtggtagcgt gctccaacac
    25261 attcaagccc ggttgcctca acctgacacg tgagtggtga aatgccgggg gtcgattgaa
    25321 ctcgcgttcg atcctgtccg gaaggtgttg ttttccatga agaagtccct gtataaaacc
    25381 cgctggtttt ggatgagtct tctttacctc ctggtcctgc ccccggcgtc ctgggcggcc
    25441 gacgactggg acttcctgga tcgagttccc ggggaccttc gaagcactgt cgggccggtt
    25501 ccgtcccccg ggttgcgtta cccgtccctg gccgccgggg aggccgaggg aattcccatt
    25561 cgagtgatca agggcgagga gggaagcctc caggtccgcg tggggatgga gtccaagccc
    25621 aacgtggata accggctcct ggagatcgag ctcttccgca tcgagcgcat ccgtgggacg
    25681 gactcccggg gaatcgaaca cgaattcctc gacgtcctcc tgccgcccac cgggaccagc
    25741 acggccgagg acgggtccat ggtcttcgac ccgggaaccg tcgccgccgg gacc
//
LOCUS       DAOOVC010000048        25503 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1305_length_25503_cov_5.915475, whole genome shotgun sequence.
ACCESSION   DAOOVC010000048 DAOOVC010000000
VERSION     DAOOVC010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 25503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25503
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1305_length_25503_cov_5.915475"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..256)
                     /locus_tag="PLM79_08245"
     CDS             complement(113..256)
                     /locus_tag="PLM79_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86339.1"
                     /translation="MVTDTAFYRDSHYHLATDTAERLRYESMTRVVSGLSRVVLDLCL
                     VKP"
     gene            complement(250..1104)
                     /locus_tag="PLM79_08250"
     CDS             complement(250..1104)
                     /locus_tag="PLM79_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOV86340.1"
                     /translation="MIWALAIGITVAAIAGILVYGTRMPERSFQGIPPPLTSDEIVLC
                     DRLRRHVHVLATEIGERNRLCYEALERARIYIGEQFSRSGLETRDLPYRFQGETFYNV
                     EVLLEGSEPSLPGVVVGAHYDSVVGSPGANDNATGVAALLELARLLAPHKPARTLRLV
                     AFANEEPPYFNTGEGMGSIEYVRSFPDPHATFFCMISLETIGQYSDQRNSQDYPPLVG
                     LFYPDRGDFIAFVGNLRSRNLVRRAVGTFRLGASVPSQGAALPSLIPGVSLSDHRSFW
                     EAGSPPSW"
     gene            complement(1360..2319)
                     /gene="bioB"
                     /locus_tag="PLM79_08255"
     CDS             complement(1360..2319)
                     /gene="bioB"
                     /locus_tag="PLM79_08255"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOV86341.1"
                     /translation="MDFKDLKEASEQGSAISPETALAVLRCHRDDIPEVLAAATRVRR
                     RYFGDRTILCSIMNAKSGACKEDCAFCAQSSHHRTRAEITPLASSEDILAAYRGVCDL
                     PVEFFGVVTSGESLSEAGVRKVCEAIREERDSRVKWCASFGCLDRDRLQILKEAGFRR
                     FHHNLETAESHYPSICGTHPYSQRLATVRAVKEVGLEICCGGILGLGESLEQRVEFAA
                     CLAREEVDSIPLNFLIPIPGTRLEQLEPMKPLDMIRNIAMFRLMNPRAEVRVCAGRVH
                     LRDLQSMVFYAGATGIMMGPLLTVAGREVKQDRKMLEDLEILP"
     gene            2610..3266
                     /locus_tag="PLM79_08260"
     CDS             2610..3266
                     /locus_tag="PLM79_08260"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOV86342.1"
                     /translation="MRLLMIDNYDSFTHNLVQLFYHFHIEVLVYRHDAVTVPEIEAMA
                     PHWICLSPGPKDPSHTGICKEVVVRLGRRIPILGVCLGMQVINEVFGGRTCRAPIPVH
                     GKRSRVHHQGLGVFRNLPSPFWAARYHSLQVIPGSDELEVVAFASDGVAMGIRHRSRP
                     IHGVQFHPESFLSEFGWELAANFLETCPAWAKISDSLPKPASTPWKPDLSFLPVPGAS
                     "
     gene            3266..4666
                     /gene="pabB"
                     /locus_tag="PLM79_08265"
     CDS             3266..4666
                     /gene="pabB"
                     /locus_tag="PLM79_08265"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOV86343.1"
                     /translation="MQTVHLSKHGLSVPCNGDEDFLRHARAIAQTPCSVVLLSGGPLW
                     DGDENRYSLAAWDPFLLYRAKSGRCELTSSRGTVSFDANPLEILDEVVGTLRPEFPLV
                     APPFSGGALGYLSYDLKNCIERLPQTALDDLDLPDLFLLWPQRILVHDRRENRFTHLE
                     MTPEGEAPSPPSFLSAIPGSSFPGALGAGTLRSNFTRPEYLDAVRAVREYILSGDVYQ
                     VNLSQRFRFPFRGDPFRLWTALFQKNPAPFYAFVNGGDHWVLSTSMERFLCRRGGVLE
                     TRPIKGTRKRGRDDAEDTALKLDLLDDPKDDAELSMIVDLLRNDLGRICAGRSVRVKE
                     HKRLETYQNVHHLVSVVEGELRPGVSLGDILRATFPGGSVTGCPKIRSMEIIDELEPT
                     VRHVYTGAIGYFGWHDNMDSNIAIRTALVHNEQCYFSVGGGVVYDSREEDEYEETLHK
                     ARTFFEVIREMGVEIR"
     gene            4663..5517
                     /locus_tag="PLM79_08270"
     CDS             4663..5517
                     /locus_tag="PLM79_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOV86344.1"
                     /translation="MKEPRASQPIVWLNGEFLPLDRAGISPLDRGFQHGDGLFETLRA
                     EAGRPLYWRDHVDRLLASASFLRISMDPSPDWRGLLREILQRNRLTEVPARIKILVSR
                     GVCEDPGLPRGCSPSLCVLAVPYLPPSPALYEKGWRLHVAQEGFSSPLSPHKTLSYLP
                     FWTARQEALDRGADEAVLRDPAGMVTETSAGSILARSAGRWWTPEARLQLPGITLKQV
                     RRLIEQAGKSVEARPCPEVELLSADTVWVLNSMVLVMPVSQIGKRPVANLELTEAASL
                     REALLEEK"
     gene            complement(5538..6383)
                     /locus_tag="PLM79_08275"
     CDS             complement(5538..6383)
                     /locus_tag="PLM79_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV86345.1"
                     /translation="MKFAFSANAFRRHTLAEAIETVARLGYAGIEIMADVPHAFPLHL
                     TDGDVRDIGRRLSDHGLEISNLNAFMHHADGDTYHPSWIEADPTLRAKRIDYTLRCIE
                     LARKLGAPTLSTEPGGPLPGISRQEGLRWFLDGLAAVEGAARDAEIRILIEPEPGLLI
                     ENSTQFREIMESLPTDVFGLNFDIGHFFCVGEDPSQLVVGLKDFTHHFHLEDIAASRE
                     HYHLIPGEGAIDFSAVLGAIRNIGFQGFVTIELYTYEDRPEEAARRALVGVRSPYGLS
                     PARGG"
     gene            complement(6380..7255)
                     /locus_tag="PLM79_08280"
     CDS             complement(6380..7255)
                     /locus_tag="PLM79_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV86346.1"
                     /translation="MKLSYSTNGFVHGPLTQAVESIARIGYRGVELLADRPHMDPCSF
                     SAGDLSRLKETLARTGIRVANINANTAAAYYGRDFWEPLFEPSLAHPDPSLRQWRIDY
                     TRRCIDLAAELDCRNVSVTSGRMQPGVPPDRSRELLEESLERVLRHARGRGVRIGMEY
                     EPGLLVERFEEWRALAAQLNSPVFGANWDVGHSHVLGEDPGEVIEGLQPRLFHVHVED
                     IRSRKHYHLIPGEGDMDLAHLMDLFARHRYEGFLTVELYTYPHRAEEAARRSLEVLRP
                     MVQGAIARRQERETR"
     gene            complement(7252..8469)
                     /locus_tag="PLM79_08285"
     CDS             complement(7252..8469)
                     /locus_tag="PLM79_08285"
                     /inference="COORDINATES: protein motif:HMM:NF019606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOV86347.1"
                     /translation="MKPSRKIGAWMIGALGSIASTVILGAVLMRKGLARPTGMITEKD
                     PFDQLDLCPVEDLEFGGFDIRDGTFLSNVRRTLDQFPGLDRNLPDRVARELAEVDSEI
                     RKGTVVNCGEAIASLASDPSPRACASLTEEVERLRACLRDFKERRGLAEVVVVNLAST
                     EPPLETPLGHADRASLENCLRTDSRESVRASTLYAYAAIGEGFPYVNFTPSSGALTPG
                     LIDLAKDCGTPVMGNDGKTGETLIKSALAPMFHDRNLEILSWEGFNLLGNMDGRVLKH
                     PENRKSKIETKDHLLPKILGYSPHSRVHIDYVPSLEDQKTAWDFIHFRGFLDARMSLQ
                     FIWQGYDSLLAAPLVLDLVRWAEYAKRNGESGLMPHLASYFKAPLGVAQHRFHAQYEM
                     LLDYARRRMNSTS"
     gene            complement(8502..8969)
                     /gene="queF"
                     /locus_tag="PLM79_08290"
     CDS             complement(8502..8969)
                     /gene="queF"
                     /locus_tag="PLM79_08290"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HOV86348.1"
                     /translation="MSDTVHCEPTQLGREVPLPKSPAEARLETIPNRGQEIDYVVRLT
                     SPEFTTLCPITGQPDFAVIIVDYVPRQRLVESKSFKLFLGSFRNQGMFHEDCTVTIHN
                     RLKEAMDPKYLRVVGLWNPRGGIPIDVVVQTGELPPRCDPLPLGGCSYRGGRQ"
     gene            complement(9006..9704)
                     /gene="queC"
                     /locus_tag="PLM79_08295"
     CDS             complement(9006..9704)
                     /gene="queC"
                     /locus_tag="PLM79_08295"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOV86349.1"
                     /translation="MRQESSPKAVVLLSGGVDSTTCLAVAKKEGFDLYALSFFYHQRH
                     GVEIEAARRVATAFRVKEHLVLDLPLNRIGGSALTDAIEVPKDTPGAAMEVGIPVTYV
                     PARNTIFLSLALGWAEVLGAADLFIGVNAVDYSGYPDCRPEFIRAFEAMANLALKATV
                     EQRMTMRIHTPLMHLSKARIIRYGAELGVDYSLTHSCYDPDPRGRACGRCDSCVLRSR
                     GFREAGIPDPAAAG"
     gene            complement(9882..10517)
                     /locus_tag="PLM79_08300"
     CDS             complement(9882..10517)
                     /locus_tag="PLM79_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV86350.1"
                     /translation="MNVGAVRPLSVLEFNLSIAVWVGLIALVGVDAETGVFMFLYLDF
                     AYYERRAKGRMKTREDLREAVVQGAVKRLRPKFMAVAVMFLGLVPILWSTGAGSDVMK
                     RIAAPMFGGIFTPFPMELAVYPAIYEVRRGYFHVRPDGRKAEEIGLGKGGSCPQDQLR
                     PCTRTGFHETDDRVDDGFVCGGFSCRFLGSWGEGPGGSSPGRLPRHELPRQ"
     gene            complement(10552..11250)
                     /locus_tag="PLM79_08305"
                     /pseudo
     CDS             complement(10552..11250)
                     /locus_tag="PLM79_08305"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(11411..11578)
                     /locus_tag="PLM79_08310"
     CDS             complement(11411..11578)
                     /locus_tag="PLM79_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86351.1"
                     /translation="MDLVPVYAEEEGASEPGDAPGSVRITSRRQQLIGVRYDEARKRP
                     LSKIIRTVVGG"
     gene            complement(11820..12467)
                     /locus_tag="PLM79_08315"
     CDS             complement(11820..12467)
                     /locus_tag="PLM79_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86352.1"
                     /translation="MALLVNEIFHSIQGESSLAGWPCVFVRTAGCNLRCSYCDTRHAY
                     EEGRLVELPDLIPQIQQYGCPLVEITGGEPLIQKETPDLIVELLDRGMCVLLETNGSQ
                     DISRVDPRCIKVVDFKCPSSGETDSIDFRNIGRLQERDEVKLVIADWNDYQFAKDIMR
                     QVMKLSFSDLKIHFSPVYGRLEPRELVSWILRDGLRVRLNLQLHKYIWGPDRKGV"
     gene            12593..13024
                     /locus_tag="PLM79_08320"
     CDS             12593..13024
                     /locus_tag="PLM79_08320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOV86353.1"
                     /translation="MSPKIMQYFKRDQFARYVGIDLLEVSEGKAVARMKIEKHHLNGV
                     GIVHGAAIFSLADLAFAVASNSHGTVAVAINVNISFLKAVSRGTLIASAEEVSRNPKL
                     GNYRIQVRNEEDELIAVFDGLVYRKKEPLPLDAADTAGDGQ"
     gene            13988..14416
                     /locus_tag="PLM79_08325"
     CDS             13988..14416
                     /locus_tag="PLM79_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86354.1"
                     /translation="MRRIEDTAARKKVPSPVTGVDEDGESLQSRTEPTPPKVYLYEEG
                     RLKPFSPGEYTRATLYQVVETFTVESKLVRPEESGTLAYEGKRPSKKTVPIVINKEGE
                     CLLPESWEALESRPEDLAHAVEVFGANPVRQVFVLRRKEE"
     gene            14424..15269
                     /locus_tag="PLM79_08330"
     CDS             14424..15269
                     /locus_tag="PLM79_08330"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOV86355.1"
                     /translation="MTTLSVQTLAGRRCRVLLWTVGILLLFQACTKRVCPVTEDAAPP
                     AASRPSSCPAPRPGSVAVAEKPLTRSSDCRTVTHEVAPLETLWRLSRMYDIPVESICS
                     ANGIKPSDPLHVGQKLVIPNTRSFRHVIALYPNRRWTHIVVHHTATDIGKALLIDRTH
                     HDRGFWHGLGYHFLIDNGTLGKGDGQIEVAPRWIKQQTGAHCKAGNMNEKAIGIALVG
                     NFNEDLPTPRQLDSLAYLLDLLTRYYRIPSNRIVGHREVPGANTDCPGSRFPWHSVRQ
                     VLTAR"
     gene            15646..16677
                     /locus_tag="PLM79_08335"
     CDS             15646..16677
                     /locus_tag="PLM79_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOV86356.1"
                     /translation="MNELLAPGGSLAMVEEVLARGADAVYVGAKGFSRRKCAWEMEDS
                     QIREAVETAKPRNGKIRVALNAEIPPERSMVLMGKIAKYASWGIEGVIVKTPAVIRMV
                     KENFPGLVIHASVGCNIQTPAQMREYREYGATQIVASTEIDTVEKLRTFKEAADRLNL
                     AVEVLIHGNRCVGGVGNCTFHELISDCYIVKKYRDEDGNEIIEYEGWPDRSGSCFRLC
                     LLTDAQRTKVLEQRGRSSEQIRSINERIRMRPNVAFAMNGKELWEVMDLGLHTLKVQG
                     REYPVSLIGRMIQLYRCFIEAHREGKPSDSPELAPLQRELDDLARDRDRARMEKTREL
                     HGNIKGLHS"
     gene            complement(16658..18046)
                     /locus_tag="PLM79_08340"
     CDS             complement(16658..18046)
                     /locus_tag="PLM79_08340"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HOV86357.1"
                     /translation="MIVRAFRRLFEGEETLLGRIGSSSWLTGYRAAMTAAWPFLVLYY
                     FARKGSGLGLGEHGLGRLGLALPSPVLNGPRIWIHALSVGETLSAVPLVQSLKALSPE
                     TRILFSSATATGWATARERLSPWVSTFFVLPHDFPWTADLLVSRLKPRRFVLVETDLW
                     PHFLRTLRRRRVPAVLVNGRLSERSYRRFLRFKPLVRSILEEFECIFPQSDGDASRFL
                     DLGVRPERIRLEGNLKFDSSRPRESREGLLDLRREVLGDQDRPAWIAGSTHPGEEEIL
                     LDVHASLRRDLPELLLILAPRQPRRGTEIGGRASARGFSWAARSTGESAVGKDVYVLD
                     TLGELGRFYGLADAAFIGGSLVPFGGHNPLEAAAVGKPTVWGSHMSNFRDMEAMLLES
                     GCAARVDSGAELLSTLRVWLGGIEMRKSMGTRAKELMDRNRGCSERIALSLLDALEDA
                     AFPGANLRNEAP"
     gene            18601..23547
                     /locus_tag="PLM79_08345"
     CDS             18601..23547
                     /locus_tag="PLM79_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86358.1"
                     /translation="MRQETRLDGSLARRWRLHLTFALILGLLAFLPAAQALGANVTVK
                     LVGEDGVTPLAIDFRYTVEEDLTFHPEANPGFPNFADQNVLSFNFHRSYMPVVSTGTG
                     AAGTATVGGLDPAKFYFISVLPSLPEKHTMGGGLVRPGQTSLTISLNNLPLPTAQISV
                     FVFEDNNRINGVADLPQEHGLEGFIVNIREAGGRYGANGELITQDAFGNPLGTTYKPD
                     GSVLAVGDGIIMTDANGLALIKNLPPAKYGVTISPPLGTNWVQTSTIEGSPTIDAWVK
                     ANEPSYFTEFGPAGHHCIFGFVRPFKDTAILSGGTTIQGQVVSDHMNRPPNYGFNAGA
                     LFPDTWVGLNDSTGKGVFAGPCDANSNFTISGVPAGTWQLVVWDKPLDVVIASTLITV
                     WPDGTCGPVRGGSCNLNKVAVFDWFAHLMGKVFYDTNQNGFQDLATDPTEVGLSQRTV
                     NIRWRDGTLYQSATTGPDGSYHFAEVFPFFNWLVAEVDFLNMKPTGLTAVVDAGGPVN
                     TAVETFPGYGRLTPQVQPVTNPNTGDNLSRTETGPVLTQPFQAFLGQTNVIEWGKVDY
                     PEGETGGIAGMVFYDTTRAEDDPYYGIGETWQPGIPNVTVNLYSVDAVTLAETYVTST
                     TTDSWDANRPTGCPGDPNDPFYMNAKCYDGMRNWNQVRPGVFDGGYGFSGLAPGIYVV
                     EVVPPPGYKLVRSQDKNVDFGDTYKPAALSRLPECVGPTYVVPAELSLFPGEPAPLAG
                     RTLNDCNRKKVYLSEAKNAAADFFLFTDAPIASHVVGMILNDLANEFDPNNPNFGEKF
                     APSWVPIGFYDYLGKEITRVYSDQYGNFNALLPGTYSVNIPSPSGVAPNMVVACMNDP
                     GPIPDPANPGEFIVDPYYSSQYSQFCYTFQYMPGATTYLDTPVLPIAAFAGQRQFPLD
                     CEFPDKTPVISYVTNPNLNGAGGRRGGGPYIPNKNANNGAQRRLQIRSMGPTLVPNPA
                     YDGTAGSPKTIVRDFGFGGTRGTVTLGNRTLDIVRWNNRLIEVEVPRGTNTGQLMVTR
                     GDNQVQTVYGITVTMGPLAQGNTAMWVNTKSSIQAALDLAKPGDLILVEPGTYNELVV
                     MYEDVYLQGYGAPSVLINAVKRPGEKLQAWRDKVASIVTAPGTNYLLPGQVLNFDPAN
                     NEPGLLNTAEGPGILVLADRRKWTPSRKPRIDGFGITGSDIGGAIVVNGNARALEISN
                     NKLFSNHGLYAGGIRVGHPDLFGADDNPIDANNWNMKVHHNFITQNGSTAQDGAGGIG
                     LFSGSAGYEVTENYVCGNFAVGSGGGIGHLGLSAHPQRANRIADNKVLFNQCFNQVPA
                     SVSEGGGISIAGVVPPPNAAGGLTAGTGSVLIDSNRIHGNLAGSGDGGGIRLAYVNGQ
                     DVLNNPGKQDNWYTVQVFNNMISNNVSGLAGGGIILKDTLKAGIIHNTIVHNDTTATA
                     GEAFPRGVLFPSSRHPAGVVSRAHSPELLALNRKGFSDPELKNNILWRNRAFFWDPSV
                     TNAQGIAVGGLLNGSAWDLGVLGRTATLNPRNCLLTSLQGIPAGNNNVTGNPRFVSTF
                     PNGASGLPVQPENTVPLTAAAVDEGGNFIDVRFGPLSLINPGTGVPYVDLRINANNSP
                     ALGAGIGNVVNTYPPLRFDYFNNNRPSNRPDIGAHERN"
     gene            23652..24896
                     /locus_tag="PLM79_08350"
     CDS             23652..24896
                     /locus_tag="PLM79_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOV86359.1"
                     /translation="MRKLELVMIPLLAVFFLGLGSVPSQAALFVQCPGDWDGDMVPNE
                     FLPDGVTPNPDYNPNVVCMHISGGDGYLRMADGTDLYAFGFGEVPPPKQVPLRRVMNR
                     QSLKARAPAPHIELQEGQELYLTLTNVGMTMRPDLFDTHSVHWHGYPNAAPMFDGLPE
                     PSPTPNMGASFTYYYYAGVPGTYFYHCHVEAAEHMQMGMIGTLHVHPSQNGTPITYGG
                     KTFTKFAYNDGDGSTGYHKDFHLQITAFDSNFHIQHEAVQPLPFADMHDNYPMFNGRG
                     YPDTINLSNNKVTPDKKILNKKGFWAQRANSLVTATSGQTILLRIGNVSTTHLYTITT
                     TLGVPMKVVGQGAAQRKSPAGVDVTYEANVLNFGGGQAYDVLIDTTGVPPGTYFIYAS
                     DLTNLSNGDEERGGLMTEVVIN"
     gene            24927..>25503
                     /locus_tag="PLM79_08355"
     CDS             24927..>25503
                     /locus_tag="PLM79_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOV86360.1"
                     /translation="MNRFSITKGLRIFGWMTVLLLGASLTAQAAIDGITGTKNPDGTV
                     VFNLVARKGNISTSDGDSFQMWGYAVGNRVQYPGPTLIVSQGDRIMVRLKNALPATAG
                     NTSITFPGHQVSARGGVAGTLTREAPPDGATQVIYEFTATEPGTYLYRSGTRPDLQVE
                     MGLVGALIVRPGGGLDPAHHAYNHADSYFDQE"
BASE COUNT         5012 a         8022 c         7701 g         4768 t
ORIGIN      
        1 caaccgtgac tcgtcggagg cgggacgttg gccgaccggg tttggtccct ttttcccggg
       61 tcgtccccac gcggcgggtt ggatcctgga tctttccccc gacgaaccca gcttacggct
      121 tcacgagaca gaggtccaag acgacccgag acaagcccga caccacgcgg gtcatggatt
      181 cgtaccggag tctttccgcc gtgtccgtgg cgaggtgata gtgagagtcc cggtagaaag
      241 cggtgtcggt caccatgaag gcggggaccc cgcctcccaa aaggaccgat gatcggacag
      301 ggacacgccg gggatcaggg aaggcaaagc cgctccttga gaaggaaccg aggccccgag
      361 acgaaaagtt ccgacggccc ttctcaccag gttccgggac cggaggtttc ccacgaaggc
      421 tatgaaatcc ccccggtcgg gatagaacag gccgaccaac ggcgggtaat cctggctgtt
      481 tcgctggtcg ctgtattgcc ctatggtttc cagcgagatc atacagaaga aggtcgcgtg
      541 cggatccggg aaggaacgta cgtactcgat gcttcccatt ccttccccgg tattgaaata
      601 gggtggctcc tcgttggcga aggcaaccag tcgcagggtc ctggccggct tgtgcggggc
      661 gagcaggcga gccagctcca ggagggccgc caccccggtg gcattgtcgt tggctccggg
      721 agagcccacc acggaatcgt aatgggctcc caccaccacg ccgggaaggg acggttcact
      781 cccctccagc agtacctcga cgttgtagaa ggtctctccc tgaaaccgat acggcaggtc
      841 cctcgtttcc agccccgacc gactaaactg ctccccgata tagatccttg cccgctcgag
      901 ggcctcgtag cacaaacggt tccgctcccc gatctccgtg gccaggacat ggacgtgccg
      961 acgcagacgg tcgcaaagaa cgatctcatc ggacgtcaac ggtggaggaa ttccttggaa
     1021 ggaacgctcg ggcatgcggg tcccgtaaac caggatccct gcgatggccg ccaccgtgat
     1081 cccgatcgcc aaggcccaaa tcatcgaccg tcgatccatt ctaccggatg aattccgtct
     1141 cgcatgcacg caaagcccgt tcacagcggc ggcaagcggg gcctcggacg catttgggac
     1201 gggcggttcg gtctcttcgg aaagggtccg ccggagtgcg gttgaaagaa gggcgccgag
     1261 cgagcgcggg acccgtgaga atgctccgaa gcgcccgcat aacctctccc ggatgcctca
     1321 ttccctttcc ctttgccggt acccttcctt tttgagaaat caagggagta tctcgaggtc
     1381 ctcgagcatc ttccgatcct gtttcacttc ccgacccgcc acggtcagga gaggccccat
     1441 catgataccc gtggccccgg catagaacac catggactgc aggtccctca ggtgcaccct
     1501 cccggcacaa accctcacct cggccctcgg gttcatcagc cgaaacatcg cgatgttgcg
     1561 gatcatatcc aggggcttca tgggctcgag ctgctcgagc cgggttcccg ggatgggaat
     1621 gagaaaattc aggggaatgg aatccacctc ctctcgggcc aggcaggcgg caaattccac
     1681 cctctgttcc agggattccc ccaggccgag aattccgccg cagcagatct cgagtcccac
     1741 ttcctttacg gcccgcaccg tggccagccg ctgagagtac gggtgagtgc cgcaaatgct
     1801 gggataatgg ctctcggccg tttccaggtt gtgatgaaac cgcctgaacc ctgcttcttt
     1861 caggatctgc aggcgatccc ggtccaggca gccaaaagag gcacaccact tcacccgtga
     1921 gtcccgctcc tcccggatgg cctcgcacac tttccgaacc ccggcttcgc tgagggactc
     1981 tccgctggtg accaccccga aaaactccac gggaagatcg caaacccctc gataagccgc
     2041 caggatgtcc tccgaggagg ccaggggcgt tatttccgcc cgggtgcgat ggtgcgagga
     2101 ttgggcgcaa aaggcacagt cttctttgca ggctccgctc ttggcgttca tgatggagca
     2161 cagaatggtc ctgtctccga aataccgacg ccgcaccctg gtggctgcgg ccagcacctc
     2221 ggggatgtcg tcccggtgac agcgcaatac ggccagggcc gtctccgggg aaatggcgga
     2281 gccttgttcg gaagcttcct tcaagtcttt gaaatccatg gagcgcatca cggttcaacc
     2341 tcacctggtt cgttcgtatt tgcatcagcc cggggcgcac gcgagaagat gaattgtagc
     2401 accctttttc ccgacaacct aggccggttt tctccaagag ggacccacgc ccccgtgtca
     2461 tgtctctgaa gccacttttc ccttgacacc tgcgcaaaac tgttccactt gcttttattg
     2521 gtcggaaaca atcaccggag tcatgccagg cgcaagccac ccagactcga tgcgggaact
     2581 gggtggcttt tttatgggag gaaaagccca tgaggctcct catgatcgac aactacgatt
     2641 ccttcaccca taacctggtg cagctgttct accatttcca tatcgaggtg ctcgtgtacc
     2701 ggcacgatgc cgtcacggtg cccgagatcg aagccatggc ccctcactgg atctgcctgt
     2761 ctcccggtcc caaagatcct tcccatacag gcatctgcaa ggaagtggtc gtgcgtttgg
     2821 gccggaggat tcccatcctg ggggtgtgcc tgggaatgca ggtcatcaac gaggtgttcg
     2881 gcggccgtac ttgccgggcg cccattcccg tgcacggcaa gaggagccgg gtccaccacc
     2941 aaggattggg ggtctttcga aacctcccat ctcccttctg ggccgcccgc tatcattccc
     3001 tgcaggtcat acccgggtcc gacgaactcg aggtggtggc tttcgcttcg gacggggtcg
     3061 ccatggggat tcggcaccga tcccggccca tccacggggt gcagtttcat cccgaatcct
     3121 tcctgtccga attcggttgg gaactggcgg caaatttcct cgagacctgc ccggcctggg
     3181 cgaaaatttc cgattccctc ccaaagcccg cttccacgcc ctggaaaccc gacctctcct
     3241 ttctcccggt tcccggtgcg tcgtgatgca gaccgttcac ttgagcaaac atgggctttc
     3301 cgtcccttgc aacggggacg aagactttct ccggcacgcc cgtgcaattg cgcaaacccc
     3361 ttgcagtgtc gtcttgctca gcgggggccc cttgtgggac ggcgacgaaa accgatactc
     3421 cctggccgcc tgggacccat tcctcctcta tcgcgccaaa agcggacgct gtgagctgac
     3481 gagctcccgg gggacggtct ctttcgacgc gaaccccttg gaaatcctgg acgaggtcgt
     3541 cgggaccctc agaccggaat tccccctcgt cgctcccccc ttttccggcg gagccctggg
     3601 ttacctttcc tatgacttga agaactgcat cgaacggttg ccgcaaacgg ccctcgacga
     3661 cctggacctg cccgatcttt tccttctctg gccccaaaga atccttgtcc acgatcgtcg
     3721 ggaaaaccgg ttcacccacc ttgaaatgac gccggagggc gaggccccct cccccccgtc
     3781 ttttctctcc gccattcccg gctcctcctt ccccggggct ctcggggcgg gaaccctccg
     3841 cagcaatttc acgcgtcccg agtacctgga tgcggttcgg gccgttcggg aatacatcct
     3901 aagcggggac gtataccagg tgaacctgtc ccaacggttc cgcttcccct ttcgaggcga
     3961 tccttttcgc ctgtggaccg ctttgtttca aaagaatccc gcgccgttct acgccttcgt
     4021 gaacggaggg gaccattggg tgctctcgac atccatggaa cgattccttt gccgcagggg
     4081 aggggtcctg gaaacccggc ccatcaaggg aacccgcaaa agaggccggg acgacgccga
     4141 ggacacggcc ctcaagctgg atctgctcga tgaccccaag gacgacgccg agctttccat
     4201 gatcgtggat ctccttcgaa acgacctggg gcgaatctgc gcgggtcgaa gcgtccgggt
     4261 caaagagcac aagcgcctcg agacttacca gaacgtgcat cacctcgtct ccgtcgtcga
     4321 aggagaactg cggcccgggg tgagcctcgg ggacatcctc cgggcgacgt tcccgggagg
     4381 gtccgtcacg ggatgtccca agatccgttc catggaaatc atcgatgagc tcgaacccac
     4441 ggtccgccac gtgtacacgg gtgccatcgg ctacttcggg tggcacgaca atatggactc
     4501 gaacatcgcc atccgcacgg ccctggtgca caacgagcaa tgttatttct cggtgggagg
     4561 cggggtggtg tatgattccc gggaagagga cgaatacgag gagacgttgc acaaggcgag
     4621 gacctttttc gaggtcattc gggaaatggg agtcgagatc cgatgaagga acccagggca
     4681 tctcaaccca tcgtttggct caacggagag tttcttcccc tggatcgcgc cgggatttct
     4741 cccctggacc ggggatttca gcatggggac gggctgttcg agaccctccg agcggaggcg
     4801 ggccgcccgc tctattggcg ggaccacgtg gaccggctcc tggcttcggc aagctttctt
     4861 cgcatctcca tggatccatc ccccgattgg cgggggctcc tgcgggagat cctccaacgg
     4921 aaccggctga cggaagttcc cgccaggatc aagatcctgg tgagccgcgg ggtttgcgag
     4981 gaccccggtc tgccccgggg atgcagcccc agcctctgtg tcctggcggt gccctacctt
     5041 ccgccctccc cggccctcta tgagaaaggg tggagactgc acgtggccca agaggggttt
     5101 tcttccccct tgtccccgca caagaccctg agctacctgc ccttttggac ggcgaggcag
     5161 gaagccctgg accgtggggc cgacgaagcc gtccttcggg accctgccgg gatggtcacc
     5221 gaaacgtccg ccggatcgat ccttgcccga agcgccggga ggtggtggac ccccgaggcc
     5281 cgtcttcaac ttccggggat cactctgaaa caggttcgaa gactcattga acaggcagga
     5341 aaaagcgtgg aagcccgccc ctgccccgag gtggagcttc tttcggccga caccgtttgg
     5401 gtgctcaatt ccatggtcct ggtcatgccg gtgtcgcaaa tcggaaaacg cccggtcgcc
     5461 aacctcgagc tcacggaggc ggcctctttg cgggaagccc tccttgaaga aaagtagggg
     5521 tcaggtctcc gtatggctta tcccccgcgt gcgggggata agccatacgg agacctgacc
     5581 cctacgaggg ccctgcgggc ggcttcctcg gggcggtctt cgtaggtata gagctcgatg
     5641 gtcacgaagc cttgaaaacc gatgttccgt atggccccca acaccgcgga aaaatcgatg
     5701 gccccttccc cgggaatcaa gtgatagtgt tcccgggacg cggcaatgtc ttcgaggtgg
     5761 aagtggtggg tgaaatcttt gaggcccacc accagttgcg acgggtcctc tcccacgcag
     5821 aagaagtgcc caatgtcgaa attgagcccg aaaacgtccg tggggaggga ttccatgatt
     5881 tcccggaact gggtgctgtt ctcgatgagc aggccgggct cgggttcgat gagaatccgg
     5941 atttcggcgt cccgggccgc tccttccacg gccgccagcc cgtcgagaaa ccaccggagc
     6001 ccctcctgtc gggaaatccc cgggagcggc cctccgggtt ccgtggacag ggtgggggca
     6061 cccagtttcc gggccagctc gatgcagcgg agggtatagt cgattcgctt ggctcgaagg
     6121 gttgggtccg cctcgatcca ggacggatgg tacgtatctc cgtcggcgtg gtgcatgaag
     6181 gcgttgaggt tcgagatctc gaggccgtgg tccgaaagcc gccgaccgat atcccgcacg
     6241 tccccgtcgg tgaggtgtag gggaaaggcg tggggaacat cggccatgat ctcgatccct
     6301 gcatatccca atcgagcgac ggtctcgatg gcttccgcca aggtgtgtcg ccggaaggcg
     6361 ttggcactaa aagcaaattt catcgggttt ccctttcctg tcttctcgcg atagcacctt
     6421 ggaccatggg ccggagcacc tccaacgacc tgcgggccgc ttcttcggcc cgatgcgggt
     6481 aggtgtagag ctccacggtc aagaacccct cgtatcggtg ccgggcaaaa agatccatga
     6541 ggtgggcaag gtccatgtcc ccctcgccgg gaatgaggtg atagtgcttg cgggaccgga
     6601 tgtcttccac gtggacgtga aaaaggcggg gctggaggcc ctcgatcact tcgcccggat
     6661 cttcccccag cacgtgactg tgtcccacgt cccagttggc gccaaagacc ggggaattca
     6721 gctgcgccgc cagggcccgc cattcctcga accgttccac caagaggccc ggctcgtatt
     6781 ccatcccgat gcgaactcct cggccccggg cgtgcctgag gaccctttcc agggattcct
     6841 cgagcagttc tcgagaccgg tcggggggca ctcccggttg catccggccg gaggtgacgc
     6901 tcacgttccg gcagtcgagc tcggcggcca ggtcgatgca tcggcgggtg taatcgatcc
     6961 gccattgccg cagggacggg tccggatggg ccagggaagg ttcgaaaagg ggctcccaga
     7021 agtccctccc gtaatacgcg gcggcggtgt tggcgttgat attggccacc cggattcccg
     7081 tccgggcaag ggtttctttc aggcgggaca ggtccccggc cgaaaaggag caagggtcca
     7141 tgtgtggccg gtcggccagg agttcgactc cccggtaccc gatacgggcg atggattcca
     7201 ccgcttgggt gaggggtccg tgcacaaaac cgttggtgct gtaggagagc ttcatgaggt
     7261 cgaattcatc cgtcggcggg catagtccaa gagcatctcg tactgggcgt gaaagcggtg
     7321 ttgggccacc ccgaggggcg ccttgaaata ggaggccagg tgcggcatca gccccgactc
     7381 cccgttcctc ttggcgtact cggcccaccg caccaggtcc agcaccaggg gagccgccag
     7441 gagggaatcg tacccctgcc agatgaactg gagggacatg cgggcatcca ggaacccccg
     7501 gaaatggatg aaatcccacg cggtcttctg gtcctcgagg gacggcacat agtcgatgtg
     7561 gaccctcgag tggggggagt acccgaggat cttcggcaag agatggtcct tggtttcgat
     7621 cttggatttt cgattctcgg ggtgtttcag gactcgaccg tccatgtttc ccaacagatt
     7681 gaagccttcc caactgagga tttcgaggtt ccgatcgtga aacatgggag ccagggccga
     7741 cttgatgagg gtctcgccgg tcttgccgtc gtttcccatg acgggcgtcc cgcagtcctt
     7801 tgccaggtcg atcagcccgg gggtcaacgc cccgctcgaa ggggtaaagt tgacgtaggg
     7861 aaatccttcc ccaatggcgg cgtaggcgta cagggtgctc gcccggacgg attcccggct
     7921 gtcggtacgc aggcagtttt ccagggaggc acggtccgca tgacccaacg gggtctcgag
     7981 gggaggttcc gtcgaggcca ggttgacgac gaccacctcg gccagccccc tcctctcctt
     8041 gaaatcccgc aggcaggcac gcagtcgttc cacttcctcc gtaagggatg cgcatgcccg
     8101 gggagaaggg tcggaagcca gggacgcgat ggcctccccg cagttgacga cggtgccttt
     8161 tcgaatctcc gaatccacct ccgccaactc tcgagccacc cggtccggga ggttgcggtc
     8221 cagccccggg aattgatcga gggtcctgcg cacgttcgag agaaaggtcc cgtcccgaat
     8281 gtcgaatcct ccgaattcca ggtcctccac gggacacagg tccagttgat cgaaggggtc
     8341 cttttcggtg atcattcccg tgggacgggc aagccccttt cgcatgagga cggcgccgag
     8401 aatcacggtg cttgcaatgg aacccagggc tccgatcatc caggccccga ttttccggga
     8461 cggtttcatg gcgcgctttt gtctcctggg caatcgagtc gtcactgcct gccgccccga
     8521 tagctgcagc cccccagggg cagggggtcg cagcggggag gcagctcccc cgtttgaacc
     8581 accacgtcga tggggattcc accgcgggga ttccagaggc cgaccacccg caggtacttg
     8641 ggatccatgg cctctttcaa ccggttgtga atggtcacgg tgcagtcttc gtggaacatt
     8701 ccctggttgc gaaagctccc caggaacaac ttgaaggatt tgctttccac cagccgctgc
     8761 cggggcacat aatccacgat gatgacggca aaatccggct ggccggtgat gggacacagg
     8821 gtggtaaatt ccggggaggt gaggcgaacc acgtaatcga tttcttgtcc gcggttgggg
     8881 atggtctcga ggcgggcttc cgcagggctt ttcggcaacg ggacctcacg ccccagttgg
     8941 gtcggttcgc agtgtacggt gtcgctcata ggatcttccc gtcttcttgg tttcctcccg
     9001 tctccctacc ctgccgcggc ggggtccggg atccccgctt cccggaatcc ccgggaccgg
     9061 agcacgcagc tgtcgcaccg cccgcatgcc ctgcctcgag ggtccgggtc gtagcaggaa
     9121 tgggtcaggg agtaatccac tccaagttcc gcaccgtacc ggatgatccg ggccttggac
     9181 aggtgcatga ggggggtgtg aatgcgcatg gtcatgcgct gttccacggt tgccttcaac
     9241 gccagattgg ccatggcctc gaaggcccgg atgaattccg gccggcagtc gggatacccg
     9301 gaatagtcca cggcattgac cccgatgaag aggtcggcgg ccccgaggac ctccgcccag
     9361 cccagggcca gcgacaggaa aatggtgttc ctggcgggaa cgtaggtgac cggaatcccg
     9421 acctccatgg cagccccggg ggtgtccttg ggcacctcga tggcgtcggt caaggcggac
     9481 ccgccgatgc ggttcagagg cagatcgaga accaggtgct ctttcacccg gaaggccgtg
     9541 gccacccgcc gggccgcctc gatttcgacc ccgtgacgct ggtggtagaa gaagctgagg
     9601 gcgtacagat cgaagccttc cttcttcgcc acggccaggc acgtggtgga atctacgccg
     9661 cccgacaaga ggacgacggc tttgggcgag gattcttgcc gcattgggat ttcccggggg
     9721 gacgaatcaa aaccggtggt caaattagcg cccggccggt tcgagatccc ccctgctcct
     9781 tcatctcctt cataaagttc ccggggtcct tcttggatcc ttcgggacat gcggaacagc
     9841 agaagcaaac ccgcttgccc cggtagcccg gaaactgttc cttattgacg gggtagctca
     9901 tgacggggca gtcggcctgg ggaactcccg ccgggacctt caccccatga gccaaggaaa
     9961 cgacaggaaa agccgccgca gacaaacccg tcatccaccc tgtcatccgt ctcatgaaac
    10021 cctgtccttg tgcaaggacg aagttggtct tgggggcaac tccccccctt tcccaacccg
    10081 atctcttcgg ccttccgtcc atccggtctc acgtggaaat acccgcgccg gacttcgtag
    10141 atcgccgggt aaaccgccag ctccatgggg aagggggtga agatgcctcc gaacatgggg
    10201 gccgcgatgc gtttcataac atctgagccc gcccctgtcg accacaggat ggggacgagg
    10261 cccaggaaca tcaccgccac ggccatgaac ttgggtcgaa gacgcttgac ggccccctgg
    10321 acgacggcct cccggaggtc ctcccgggtc ttcattcgcc ccttggcccg cctttcgtaa
    10381 taggcaaaat ccaggtagag gaacatgaag accccggttt ccgcgtccac ccccacgagg
    10441 gcgatcaatc ccacccaaac ggcgatgctc aggttgaatt caagaacgct gaggggtcgg
    10501 acggcgccga cgttcatgct cccgagggcg gacttgagtc tgctttggga atcaatgagg
    10561 aaatgggcgg aaacgacccc cccgttcccc cgcctcgacg cccgccatca caatgacatc
    10621 ctccccgacc gggggtcccg gggagacctt ccgcggctcg agggactccc cttcccgggc
    10681 cgcaaagacg acctgttccg tgcccgaatc caggacgggc tcccggggca cggccggctg
    10741 aacccccaag tccgtctcga ctcccatgtt cgcgtatatg tccggtttga gctgatagcc
    10801 gggattggga aattcgatct tcgccttgag gctgcgggtg gaaggctcca gccccgggga
    10861 gagataggca accttgccgc gaaaggtctt gccgggaaag caggagaggg ttatggttgc
    10921 gctctgtccc atgcggacca tgggcaattc gtattcgtag acgtgggctt aacccaaacg
    10981 gtggaaaggt ccgcaaggct gtagaactcc atctccggcg tgactcggcc cggcgaaccc
    11041 gttgcgggtc gggacaggac cgtggatggg agaatacagg gtcaggccct tgccgggggt
    11101 cccccgtttt tcgatctccc ggacttcgga ttgggggatg tcccaaagct tcagcctttc
    11161 tcgagtgaat tcatagagcg tccgggcatt ggaccccagg ctccgcaaaa aaggactcct
    11221 ttccagggaa tccttggact tctttgcaat cagcagatcc taccgggtcg agaccaactc
    11281 ggaactgtat gtaaatgatt agaagagaag cggccggccc ttctcagcat tcccgtgaag
    11341 tccacgtaca cctggtcgat ccagccggaa acccgggcat gaatatgggc ggtcctggtt
    11401 tcatcgtagg tcacccgccc accacggtac ggatgatctt ggacaggggc cgtttccgcg
    11461 cctcgtcgta tcgaaccccg atgagctgct gtctccggga agtgatccga acggagccgg
    11521 gagcgtctcc gggctccgag gccccctcct cctcggcgta gacggggacg aggtccatgc
    11581 cgcagggggc ttttcccggc tcgatccgcc aagggagaga agaaagcctt ctcgacctcg
    11641 gaaagacggg gcccgagtga ggggatccct gttgacggat cggtccttgc ccggcaccga
    11701 tgggattcgg gcggaaggga gcaggaaaaa tggaacggtt cgggcctctg ttggcggatt
    11761 cggcggcggg gggacggacg aaggaagaag gcctgcgggg tccaaaagaa atcagtctcc
    11821 tacactccct tccgatccgg tccccagatg tacttgtgga gctgcaggtt caggcggacc
    11881 ctcaacccgt cccgcagaat ccacgaaaca agttcccggg gctccagcct cccgtacacg
    11941 ggagagaaat gaatcttcag gtctgaaaag gaaagcttca tgacctggcg catgatgtcc
    12001 ttggcgaact ggtagtcgtt ccagtcggca atcaccagtt tcacttcatc tcgttcctgc
    12061 agtctaccga tgtttctgaa gtcgatggag tcggtctccc cgctcgaggg acacttgaaa
    12121 tccacgacct tgatgcaccg ggggtccacc cgggagatgt cctggcttcc gttggtttcg
    12181 agcagcacac acatgccgcg gtccaggagt tccacgatga ggtccggggt ctccttctgg
    12241 atgagcggct cccctccggt gatttccacc agcgggcagc cgtactgctg gatctgggga
    12301 atgagatcgg ggagctccac gagacgacct tcctcgtagg catgccgcgt atcgcagtag
    12361 ctgcagcgca ggttgcagcc ggccgtccga acgaagacac agggccaccc ggcgagagaa
    12421 gactctccct ggatgctgtg aaaaatctcg tttaccagaa gtgccaatcg ttccccctgc
    12481 catttacggg ttcattttct cggtcccttg caatataacg gaaggccgat cgggttgtcc
    12541 acgtcttccg cggcaaccct cgaggggagt atccggaata tagggaggac cgatgagtcc
    12601 aaagatcatg cagtatttca agagggatca atttgcccgg tacgtgggca tcgatctgtt
    12661 ggaggtttcc gaggggaaag ccgtcgctcg gatgaagatc gagaaacatc acctgaacgg
    12721 tgtggggatc gtccacggag ctgcgatctt ttccttggcg gatcttgcct tcgccgttgc
    12781 gtccaattcc cacgggacgg ttgccgtggc gatcaatgtg aacatctcgt tcctcaaggc
    12841 cgtgtcccgg ggaaccctca tcgcctcggc cgaggaggtt tcccggaacc ccaagctggg
    12901 aaactaccgc atccaggttc gcaacgaaga ggacgaactg atcgccgtct ttgacggtct
    12961 ggtgtaccga aagaaggaac ccttgcctct cgatgccgcg gacaccgcag gggacggcca
    13021 gtaaggggtc aggtctccgt ttggctgacc ttaagggtcg tgaccttagg ggtcgagtct
    13081 tcgtatggcg tgtcccgtgc gtgcggagga gagagtgtag ggggccctac caggcgtgtg
    13141 gggcgcagtc ccgctcttgt tcacgggacg gtcccgcgac cccatgggcc gccttcctct
    13201 tgggatccgt tccgggggta tgggccttgc ggcctcgaat tccggctcat ggctgcgaac
    13261 ccaccgggtt cgctgggagg cggcgatcta tccccgcctg atcgcaaatg tgggtaaagg
    13321 gcagtcctgg ccggaggctt ggggtcctgc gggattgtcg agccctgaga acccgccgcc
    13381 ccccggggac gaatgggctt catcgccgct tcccgaggcg ggatccggag ccggggccat
    13441 catggatgcg cccggtcgtt ttgccgccga gtcccggagg tgggtttcgg cgactcgtcg
    13501 acgggcacgg gagggggaat ccgcagggat ggaccgcgcc gcccggctcg aatttccggt
    13561 tggttttttg agggttgtga tctatgctgc cggggaggtt ccgtgcaggc atgacgcgat
    13621 ttttcgggtt ttccggcgct tgtttctcgg tacgccgaac ttgggtctct aagcactcgg
    13681 ggatccgctc ccggccttat ccccgggcaa tttgcggaac ccagggtccg gttccggcgg
    13741 cggctttcag gcaaccggtt cccatcccgg gcaagcgccg acttgccggg ggccaatcct
    13801 tcgattcacg ttacggtcaa ggtccatggc agaattcgtt tggtgcaaga aagaagagtg
    13861 ccgcctgccc cattttcgat gcctcttgtg ccgggaggac tgctactcga ggggtgagtc
    13921 cgccgggcaa tttgattccg ccctcgaggg tctgattcga tcgggaagat acaaggagcg
    13981 tttcgtcatg agacgaattg aagacactgc tgccaggaaa aaggtcccgt ccccggtgac
    14041 cggggtagac gaggacgggg aatctctgca atcgaggacg gagccgacgc cgcccaaggt
    14101 ttatctctac gaggagggaa ggctgaagcc cttttctccc ggggaataca cccgggcgac
    14161 cctctaccag gtggtggaaa ccttcaccgt ggagtccaag ctcgttcgcc ccgaagaatc
    14221 cggcaccctg gcctatgaag ggaagagacc ttccaaaaag accgtgccca tcgtgatcaa
    14281 caaggagggt gaatgcctcc tgccggaatc ctgggaagct ctcgagtccc gccccgagga
    14341 cctggcccac gcggtggaag tcttcggagc caaccccgtt cgccaggtct tcgtgctgag
    14401 aagaaaagag gaatgacgac ccattgacga ccctgtccgt acaaaccctt gccggaagga
    14461 gatgcagggt cctgctgtgg acggtgggaa tcctgctgct ctttcaagcc tgtaccaaga
    14521 gggtttgtcc cgtcacggag gatgcggcgc cccctgcggc ttcccgtccc tccagctgcc
    14581 ccgcaccccg acccggaagc gtggcggtgg ccgaaaaacc cctcacgcgc tcctcggact
    14641 gccggaccgt cacccacgaa gtcgctcccc tggaaaccct ctggcgtctt tcccggatgt
    14701 acgacatccc cgtggaatcc atctgttccg ccaacggcat caagccttcg gatcctctcc
    14761 atgtgggtca aaagctcgtc atccccaaca cccgttcctt tcgccatgtc atcgccctct
    14821 accccaaccg gcggtggact catatcgtgg tgcaccatac ggccacggac atcggaaagg
    14881 ccctgctcat cgaccgcact caccacgatc gcggcttctg gcacggcttg gggtaccatt
    14941 tcctcattga taacggaacc ttgggaaagg gagacggaca aatcgaggtg gctccccgat
    15001 ggatcaagca gcagacgggg gcccattgca aggcgggcaa catgaacgag aaagccattg
    15061 gaattgccct ggtggggaat ttcaacgaag atctcccgac tccccgacaa cttgactctc
    15121 tggcctacct gctggatttg ctcacccggt actaccgcat cccctccaac cgcattgtcg
    15181 gccatcggga agtgcccggg gccaacaccg actgcccggg gtcccgcttc ccgtggcaca
    15241 gcgttcgcca ggttctcacc gcccggtagg aagtcagggg ctcgatcccc gagaagaaaa
    15301 gcctgcgccc cggcagacgg ttattcgcaa caacctcacg ccctcactgt gcatttttgg
    15361 aatttgaggg gatatgggtc tttaaatgcg ggttctttgt gtgttatatt tttagcaatg
    15421 tgcattaccg tcgctccgca tgaaaggagc accctgtatt ctccgcaggg cccgtcggcg
    15481 gcttgaatcg ggaagacggg aggtcgagga ttcgagtcac gcgagggtgc tcacgggatc
    15541 ttggtcccgt ggggatgacg gtgcggggtc ggtgcctcgc acggtccctc tttaccgaag
    15601 ggatggcagc ccggtcggaa gaagtcgaaa gggagggagg aagccatgaa cgagctcctg
    15661 gcgccaggtg gaagcctagc aatggtggaa gaagtccttg cccggggtgc cgatgcggtc
    15721 tacgtcggcg ccaaggggtt cagccgacgc aagtgcgcct gggaaatgga agactctcaa
    15781 atccgtgaag ccgtcgagac ggccaaaccc cgcaacggga agatcagggt agccctcaat
    15841 gccgaaattc cccccgaacg atccatggtt ctcatgggga agatcgccaa gtatgcctcc
    15901 tgggggatcg aaggcgtcat cgtaaaaacc cccgccgtga tccggatggt caaggagaac
    15961 tttccaggac tggtgatcca cgccagcgtg gggtgcaaca tccagacccc cgcccaaatg
    16021 agggaatacc gggaatacgg tgccacccag atcgtggcca gcacggaaat cgatacggtg
    16081 gaaaagctga ggaccttcaa ggaagcggcc gaccgtttaa acctggccgt ggaagtgctc
    16141 atccacggca atcgttgcgt ggggggagtg ggaaactgta ccttccatga gctcatcagc
    16201 gactgttata tcgtgaaaaa gtaccgggac gaggacggga acgagatcat cgaatacgaa
    16261 ggttggcccg atcggagcgg cagctgtttc cgcctttgcc ttctcaccga tgcccagagg
    16321 accaaggtgc tcgagcagcg gggacgcagt tccgagcaga ttcgttccat caacgagcgg
    16381 atccgcatgc gccctaacgt cgccttcgcc atgaacggca aggaactttg ggaggtcatg
    16441 gatctcgggc tgcacacctt gaaggttcag ggtcgggaat atcccgtgag cctcatcggg
    16501 cgaatgatcc agctctaccg atgtttcatc gaagctcatc gggaaggaaa gccctctgat
    16561 tcgcccgagc tggcacccct gcagcgggaa ctggacgatc tggccaggga ccgggaccgg
    16621 gcccggatgg aaaagacccg ggagctccac ggaaacatca aggggcttca ttcctgagat
    16681 tggcccccgg aaaggcggca tcctcgaggg catccaggag tgacaacgca attctttcgc
    16741 tgcatccgcg gttcctgtcc atgagttctt ttgcgcgggt ccccatggat ttgcgcattt
    16801 cgatgccccc caaccacacc cgcagggtgg agaggagttc cgcgcccgaa tccacccttg
    16861 cggcacatcc cgattccagg agcatggcct ccatgtcccg gaagttcgac atgtgggacc
    16921 cccacacagt cggttttccc accgccgcag cctccagggg attgtgcccc ccgaagggca
    16981 ccaggctccc cccgatgaag gcggcgtccg ccagcccgta aaatcgaccc aactcgccca
    17041 gggtatccag cacgtaaaca tcctttccca cggcggactc ccctgtcgat cgggcggccc
    17101 acgagaatcc ccgggccgag gcccggcctc cgatttcggt tccccttcga ggttgacgcg
    17161 gggcaaggat gagcaggagt tccggcaggt ccctccgaag gctcgcgtgc acatcgagga
    17221 ggatttcttc ctcgccggga tgggtgctcc cggcaatcca ggcgggacgg tcctggtcgc
    17281 ccagcacctc cctgcgaaga tcgaggagcc cctcccgtga ttctcgggga cgcgacgagt
    17341 cgaacttgag gtttccttcc aagcgaattc tctcgggtct tactcccaga tccagaaacc
    17401 gggaagcatc tccgtccgat tgagggaaga tgcactcaaa ttcctccagg atggaccgca
    17461 ccaggggctt gaaccgcagg aaccggcggt aggatcgctc cgagagcctt ccgttgacga
    17521 gaacggcggg gacccggcgg cggcgcaggg tccgcagaaa atgaggccac aggtccgttt
    17581 ccaccaggac gaaccggcgg ggttttagcc gggaaaccag caggtccgcg gtccagggga
    17641 aatcgtgggg caggacaaaa aaggtggaaa cccacggact caggcgttcc cgggccgtcg
    17701 cccagccggt ggcggtggcg ctggaaaaca ggatccgagt ttcgggagaa agggccttga
    17761 ggctttggac cagggggacc gcggaaagag tctctcccac ggagagggca tggatccaga
    17821 tgcgcggacc gttgaggacc ggggaaggaa gcgccagtcc caatcgtccc aatccatgct
    17881 ccccgaggcc gagtccgctc ccctttcgtg cgaagtagta caggacaagg aacggccatg
    17941 cagcggtcat ggcggcccgg tagccggtca gccacgaaga tgagccgatt ctccccaaca
    18001 aggtctcctc cccttcgaag agtcgacgga atgctcgaac aatcatcgct tctctctggg
    18061 aaccaaattg ggtggaattg tatagggatc cgtcccggat ggcaattccc ggacacccgg
    18121 ccccctctac tgtccgggtg taggacgccc gtagggtctc aaggtacccg aaacgaagcg
    18181 ctttaccctg ccgggcctcc ggcgaaaacc ccggcaaatc cgcatgcccc gcttcgatgc
    18241 acggatgatg cggggaaggt gatacccttc ggacgcagtc tccgaccccc cactccccat
    18301 actgtccctc ccgcccccta cagtccgacc ccacagcaaa tacggtgtgc gccgtattga
    18361 acgactgtcg ttttcgtgga aagtagcgaa caaggcagag ggaaagcgcc tcgaccgccc
    18421 ggagagggtc cggaacgggc ggcaactgtt cctggagagg atgttcgagc catcaagcga
    18481 accgcgacac cgggtttgac gaaggattca tgaacagaag ggagcgtgac cggtttcaag
    18541 aaagagtctt tcaggggtgg attggatttc gggtttcaac caatgttgag gaggacaatg
    18601 atgaggcaag agacacgatt ggacggctct ctggcccgaa gatggagact ccatcttact
    18661 tttgccctta tcctggggct tttggcattc ctgccggcag cccaagccct aggggcgaac
    18721 gtaaccgtga agttggtggg agaggacggc gtaacgcccc tggccatcga ttttcgctac
    18781 acggtggagg aagacctcac ctttcatccg gaggccaacc ccggctttcc gaattttgcg
    18841 gatcagaacg ttttgtcatt taactttcac cggagctaca tgccggtggt gtccacgggc
    18901 accggggccg ccgggacggc taccgtcggc gggctggatc cggccaagtt ctatttcatc
    18961 tccgtccttc cttccctgcc ggaaaagcac accatgggcg gcgggctcgt aagacccggc
    19021 cagacttccc tcaccatctc gctgaacaac cttccccttc ccacggcgca gatttcggtc
    19081 tttgttttcg aggacaacaa ccggatcaac ggggtggcgg atcttcccca ggagcacgga
    19141 ctggaaggct tcatcgtgaa catccgggaa gccgggggtc gttacggagc caacggggaa
    19201 ctcatcaccc aggacgcttt cggcaatcct ctgggcacca cctacaagcc ggacggatcc
    19261 gtgctggccg tcggggacgg catcatcatg accgacgcca acgggctggc cctcatcaag
    19321 aaccttcctc cggccaagta cggggtcacc atctctcccc ccttgggcac gaattgggtt
    19381 cagacgagca ccatcgaagg ttcgcccacc atcgacgcct gggtcaaggc caatgagcct
    19441 tcttacttca ccgagttcgg acccgcgggg catcactgca ttttcgggtt cgtgcgtcct
    19501 ttcaaggaca cggccattct gtcggggggg accaccatcc agggccaggt ggtgagtgat
    19561 cacatgaacc ggcctcccaa ctacggattc aatgccgggg ccctctttcc cgacacctgg
    19621 gtcggcttga acgactcgac gggcaagggc gtgtttgcag gcccctgtga tgccaacagc
    19681 aactttacca tcagcggcgt tccggcgggc acctggcaat tggtcgtctg ggacaaacct
    19741 ttggatgtcg tcatcgcatc gaccctgatc accgtctggc ccgacgggac ttgcggcccg
    19801 gtccgggggg gatcgtgcaa cctcaacaag gtggcagtct tcgactggtt tgcccacctc
    19861 atgggcaagg tcttctacga cacgaaccag aacgggttcc aggatctcgc gacggatccc
    19921 accgaggtcg gcttgagtca gcggaccgtc aatatccggt ggagggacgg gaccctctac
    19981 cagagtgcca ccacgggtcc cgacgggtcc tatcatttcg ccgaggtgtt tccgttcttc
    20041 aattggctgg tggccgaggt ggacttcctg aacatgaagc ccacgggtct tacggccgtc
    20101 gtggatgcgg gcggtccggt gaataccgcc gtcgagactt ttcccggata cggccgcctg
    20161 actccgcagg tccagcccgt gacaaacccg aacacgggtg acaacctgtc ccgaacggaa
    20221 acgggtcccg tcctgaccca gcctttccag gccttcctgg gccagaccaa cgtcatcgag
    20281 tgggggaagg tggattaccc ggaaggggaa accggcggaa ttgccggaat ggtcttctac
    20341 gacaccacca gggccgagga cgatccttac tacggaatcg gcgagacctg gcagccggga
    20401 attcccaacg tgaccgtaaa cctttacagc gtggacgcgg tcaccctggc cgaaacctac
    20461 gtgacctcga ccaccacgga cagttgggac gccaaccggc ccacgggctg ccccggggac
    20521 cccaacgatc ctttctacat gaatgccaag tgctacgacg gcatgaggaa ctggaaccag
    20581 gtgcgccccg gagtcttcga cggaggatac ggcttctcgg gcctggcccc gggaatctac
    20641 gtcgtcgaag tggttccgcc ccccggatac aagctggtga ggtcccagga caagaacgtg
    20701 gacttcggcg acacctacaa gcctgccgct ttgagtcgcc tgcccgagtg cgtaggaccc
    20761 acctacgtgg tgcccgccga gctgtccctt tttccgggtg agcccgcccc cctggcaggt
    20821 cggaccctca acgactgcaa tcgcaagaag gtatacctgt ccgaagccaa gaacgccgcg
    20881 gcggacttct tcctgttcac cgacgctccc attgcctccc acgtggtggg catgatcctg
    20941 aacgaccttg ccaacgagtt cgatcccaac aacccgaact tcggggaaaa gttcgcgcct
    21001 tcctgggtac ccatcgggtt ctacgactac ctgggcaagg aaatcacccg ggtctactcc
    21061 gaccagtacg gcaatttcaa cgccctgctg cccggcacct actcggtgaa catccccagt
    21121 cccagcggcg tggcccccaa catggtggtg gcctgcatga acgaccccgg ccccatcccc
    21181 gatccggcca acccgggcga attcatcgtc gacccctact acagttccca gtacagccag
    21241 ttctgctaca ccttccagta catgccgggg gcaacgacct acctggatac tcccgtgctg
    21301 cccatcgccg cgtttgccgg gcagcggcag tttcccctgg attgtgagtt ccccgacaag
    21361 accccggtca tctcttacgt caccaacccc aatttgaacg gggccggcgg caggaggggc
    21421 ggcggaccct acattcccaa caagaacgcc aacaacgggg cccagcgacg gttgcagatc
    21481 cggtccatgg gacccaccct cgttcccaac cccgcctacg acggaacggc cgggagcccc
    21541 aagaccattg tccgggattt cggctttgga ggcacgagag gcacggtcac tttgggcaat
    21601 cggaccctgg acattgtccg gtggaacaat cggttgatcg aggtcgaggt ccctcggggc
    21661 accaacaccg gtcaactcat ggttacccgg ggagacaacc aggttcaaac ggtctacggc
    21721 atcaccgtga ccatggggcc ccttgcccag ggcaacaccg ccatgtgggt gaataccaag
    21781 agctccatcc aggccgctct cgacctggcg aaaccggggg acctgatcct ggtggaaccg
    21841 gggacctaca acgagttggt ggtcatgtac gaagatgtgt acctccaggg atacggcgcc
    21901 ccctcggtcc tcatcaatgc ggtgaaaagg cccggtgaga aactccaggc ctggagagac
    21961 aaagtcgcct ccatcgtgac cgcccccggc accaattacc tgctgccggg acaggtcttg
    22021 aacttcgatc ccgccaacaa cgagccgggc ctgctcaata ccgccgaagg gccgggaatc
    22081 ctggtgctgg cggatcggag aaagtggacc cccagccgga agccgcgcat cgacgggttc
    22141 ggcatcacgg gctccgacat cggcggagcc atcgtggtga acggcaacgc ccgggccctc
    22201 gagatcagca acaacaagct cttcagcaac catgggttgt atgcgggcgg catccgtgtg
    22261 ggtcatcccg atctcttcgg cgccgacgac aaccccatcg acgccaacaa ctggaacatg
    22321 aaggtccatc acaatttcat cacccaaaac ggctccacgg ctcaggacgg agcgggcggc
    22381 atcgggctct tctccggaag cgccgggtat gaagttaccg agaactacgt gtgcggaaac
    22441 ttcgccgtag gcagcggggg cggcatcggt cacctgggat tgagcgctca tccccaaagg
    22501 gccaaccgaa ttgccgacaa caaggtcctg ttcaaccagt gtttcaacca ggtgccggcc
    22561 tcggtgtccg aaggcggtgg gatctccatc gccggggtgg ttccgcctcc caatgcggcg
    22621 ggcggtttga cggcagggac gggatcggtc ctcatcgatt ccaaccggat tcacgggaac
    22681 ctggcgggct cgggagacgg cggcgggatt cgactggcct acgtcaacgg ccaggacgtg
    22741 ctgaacaacc ccggcaaaca ggacaactgg tataccgtcc aggtgttcaa caacatgatt
    22801 tccaacaacg tgtccggtct ggccggcggc gggatcatcc tcaaggacac cctcaaggcc
    22861 ggcatcatcc acaacaccat tgttcacaac gataccacgg ctacggccgg agaagccttc
    22921 ccgcggggag tcctcttccc gtcctcgcga catcctgcgg gagtagtgtc ccgggcccac
    22981 agtcctgaac ttctggcgct caaccgcaag ggcttctcgg acccggaact caagaataac
    23041 atcctctggc gaaaccgcgc cttcttctgg gaccccagcg tgaccaacgc ccaggggatc
    23101 gccgtgggcg ggctcctcaa cggaagtgcc tgggacctgg gagtcctggg aagaaccgcc
    23161 accctgaacc ccagaaactg tctgctgacc agcttgcagg ggatcccggc cggaaacaac
    23221 aacgtcacgg gaaatccgcg gtttgtcagc accttcccca acggcgcttc cgggttgccg
    23281 gtgcagccgg agaacacggt gcccttgacg gcggcggccg tggatgaggg cgggaacttc
    23341 atcgatgtgc gcttcgggcc cctgagtctc atcaatcccg ggaccggagt tccctacgtg
    23401 gacctgcgca tcaatgccaa caactccccc gccctgggag ccgggatcgg gaacgtggtg
    23461 aatacctatc cgccgttgag gttcgactac ttcaacaaca accggccttc caaccgcccg
    23521 gatatcgggg cgcacgagcg caactgattc ccggttcgag caaagaacca tcggcccaac
    23581 cgggccgaca acgattgtga ccttccaccc gccgccctgc ggcgggtgga accaacaaga
    23641 ggagtaaaac catgagaaag ctggaactgg tcatgatccc gctcctggcg gtgttctttc
    23701 tcggcctggg atcggtgcct tcgcaggcgg ccctcttcgt gcagtgcccc ggcgactggg
    23761 atggagacat ggtgcccaac gagtttctgc ccgatggggt gaccccgaat cccgactaca
    23821 atcccaacgt cgtgtgcatg cacatctccg gcggggacgg gtatctcagg atggccgacg
    23881 gcaccgacct ctatgccttc ggcttcggag aagtgcctcc ccccaagcag gttccgttgc
    23941 gcagggtcat gaatcggcag tccctcaagg cccgggctcc cgctccccac atcgaactcc
    24001 aggagggtca agagctgtac ctgaccctca ccaacgtcgg aatgaccatg agaccggatc
    24061 tcttcgatac ccactcggtt cactggcacg ggtatcccaa cgcggcgccc atgttcgacg
    24121 gtcttcccga accctccccg actcccaaca tgggggcctc cttcacctac tactactacg
    24181 ccggggttcc cgggacctac ttctaccact gccacgtgga ggccgccgag catatgcaga
    24241 tgggcatgat cgggactctg cacgtgcatc cttcccagaa cggcacaccc atcacctacg
    24301 ggggaaagac cttcaccaag ttcgcctata acgacggcga cgggtccacg ggctatcaca
    24361 aggattttca cctgcagatc acggccttcg acagcaactt tcacatccag catgaggcgg
    24421 tacagcccct gcccttcgcg gacatgcatg acaactatcc catgttcaac ggccgcggct
    24481 atcccgatac catcaacctc tccaacaaca aagtcacacc ggacaagaag atcctgaaca
    24541 agaaagggtt ctgggcacag agggccaatt cccttgtcac ggcaacctcg ggacagacga
    24601 ttcttcttcg catcggaaac gtttccacca cccacctcta caccatcacc accaccctgg
    24661 gggttcccat gaaagtggtg ggccaggggg ccgcacaacg caagagcccg gcgggagtgg
    24721 acgtgaccta tgaagccaac gtcctgaatt tcgggggcgg gcaggcctac gacgtcctca
    24781 tcgacaccac gggggttcct ccgggaacct acttcatcta cgcctcggac ctcaccaacc
    24841 tgtccaacgg ggatgaggag cgcggcggac tcatgaccga agtcgtgatc aactaggcga
    24901 agaccctatc atcgaggagt atccccatga atcgattctc aataacgaaa ggactccgga
    24961 ttttcggctg gatgaccgtc ctgctcctgg gcgcgagcct caccgcccag gccgccatcg
    25021 acggcatcac gggaaccaag aacccggacg gaacggtggt tttcaacctg gtggcccgca
    25081 aggggaacat ctccaccagc gacggggaca gctttcaaat gtggggctat gccgtgggga
    25141 accgggtcca gtaccccggc ccgaccctca tcgtaagtca gggggaccgg atcatggtgc
    25201 ggctcaaaaa cgcccttccc gccacggcgg gaaacacctc catcaccttt cccggccacc
    25261 aggtttcggc tcggggcgga gtggcgggga ccctcacccg ggaggccccg cccgacggag
    25321 ccacccaggt gatctatgaa ttcacggcga cggagccggg tacctacctg tatcggagcg
    25381 gaacccggcc ggatctccag gtggaaatgg gcctggtggg ggccttgatc gtgcggcccg
    25441 gcggcggcct ggaccccgcc caccatgcct acaaccacgc ggattcctac ttcgaccagg
    25501 aat
//
LOCUS       DAOOVC010000049        24621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1375_length_24621_cov_7.238460, whole genome shotgun sequence.
ACCESSION   DAOOVC010000049 DAOOVC010000000
VERSION     DAOOVC010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1375_length_24621_cov_7.238460"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="PLM79_08360"
     CDS             complement(<1..918)
                     /locus_tag="PLM79_08360"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOV86361.1"
                     /translation="MRQPKFIFITGGVLSSLGKGLAAASIGALMESRGLKVTLQKLDP
                     YINVDPGTMNPFQHGEVFVTDDGAETDLDLGHYERFTHARMSKNNNYTSGSIYYSVIT
                     KERRGDYLGGTVQVIPHVTDQIKSCIRGAVNGVDVAIVEIGGTVGDIESLPFLEAIRQ
                     FRNDVGKENVLYIHLTLVPFMKTSGELKTKPTQHSVKELLGIGIQPDILLCRTERLLP
                     GDLKAKIAHFCNVEPDAVITAQDVDCIYEVPLLFHKEGLDEKIVRLLNIWTRAPVLDQ
                     WDQLVQRMKKPSHEVRIAIVGKYIDLKESY"
     gene            1268..1876
                     /locus_tag="PLM79_08365"
     CDS             1268..1876
                     /locus_tag="PLM79_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOV86362.1"
                     /translation="MIVGQWMTTKIVTIRKESSIQEALAVMKRESIRHLPVTDEDGNL
                     LGWVTDADLRGVLIASMLEELTLEDVMVRKPYVAGPAMPLEEAARIILEKKIGGLPVV
                     ADGKLVGIITVVDILTAFITMIGMLSQSSRLDVRITKPGTPLDEITRLIRHHRAEVIS
                     ICHLPGQERQDPIYSVRLKKCDLQPIVSDLLDNGIEVVSSIP"
     gene            2260..3897
                     /locus_tag="PLM79_08370"
     CDS             2260..3897
                     /locus_tag="PLM79_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOV86363.1"
                     /translation="MGKIPENYLPPKETWPVYLTPDEFADIPHQLNLADFLLDRHVRE
                     GRGENPAIKFMDKTITYGQLQQMVNRFGNALKAVGVEPQDRVGIRLVNSPQALVTIFA
                     IEKIGAVPVPTSPLWSAEEVGFVANNAEMKFFVVNAPLMEPVTKAKPEFEYGTRIIVI
                     GGNPNEVKAAGDLVYEEMVEKGSPDLEATMLNADDIGIMLYTSGTTGMPKGCVHFVRP
                     AVMVSRVVNRYVYQMKPGDILGGAAPVSFAAGFGTFSLIPFEGGAAVSLIPRFAPPDM
                     MDLIQKHRITILTGLPTAYRALMKFPDFKKYDIGSVRLYTSGGDALGAETLQAWQELS
                     GKPIWEGLGGTEMLHLVTSNTMNPEPVPNSIGKPLPGVKVKVVNAERQECKPGEIGSM
                     ILQGPSGTLYWKPYEEDEKLLRSQKKGVVDGWNQMGDAVYMNEDGNIFFVSREDDMIK
                     SSGYRIGPAEVEEAILKHPAVADAGVVGIPDPDKGQITKAVVVLKPGYQGGDAFFEEL
                     KEFLKQYIAIYKLPRVIQYMDALPRTPTGKLLRRKLR"
     gene            3981..4829
                     /locus_tag="PLM79_08375"
     CDS             3981..4829
                     /locus_tag="PLM79_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV86364.1"
                     /translation="MDWKITVLCENTVASPGFLGEHGFAALVETPEETILFDTGQGFG
                     LVQNALRLQKDLRRVTRLVLSHGHFDHTGGLLSFLGIHGPCDVTAHPDVLVERFRWMP
                     VGSEEKPLSIGIPWHEAYLTTRGARFRWQREFAEISPNVFISGEVPRKTAFETGDPKF
                     VVRKNGQWAPDPFLDDYSLFVVTPRGLIVLLGCAHAGLINILNHAVEKTGEERIHAIV
                     GGTHLGFSPEVQTEDTLRALRKYRIEVLATSHCTGQRPVARLAAEFGDRFAFGHVGFV
                     LGVFSG"
     gene            4944..6125
                     /locus_tag="PLM79_08380"
     CDS             4944..6125
                     /locus_tag="PLM79_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86365.1"
                     /translation="MQRFRAGLGILLWLFLFGFHAEAFSIRIGEINPLTGKLAEHGTD
                     IHEGILFAVEEINARGGIHGSPVELISRDDQSQPEVAVNQAEELLYRSRVVGLVGGYV
                     DSLVGPISELAAKHRVPYVAAASLQRSLTLRRSNPFFFRVSQQKGIVQPLCDFILEQA
                     RAGRVAVLYAATPGSSEFGEDVKGLLEKRGVQVPLFEKFRPGSPDFSSFLLKIRQHKP
                     DVLVSGGFFPDHLVLIRQSREQQVPFRFYIGPWGIAHPSFIREMGSMSEDLLGMCAWN
                     PGITLPGTEGESARFVEGFSRRFGKVPNSTTMHGYTSARALLAAMEGAAQKGVSWTGE
                     AISRELRSLDMLLPMERLAFDENGDPRYYQQVIVQIRNGQMVPVFPQDRAAATVRFPR
                     D"
     gene            6343..7209
                     /locus_tag="PLM79_08385"
     CDS             6343..7209
                     /locus_tag="PLM79_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV86366.1"
                     /translation="MWLQLALNAFTLGSFYALIALGFSLIFGITRAFNLAHGEMVLLG
                     GYLAYVLGTYWQVPFFWTLPACMLTLLLLAVLLQALLQHVREPFELNSLVVTFGLALV
                     LQNGMLVLFSANHRLIRNAEAAWSLPSLHLTLTQLQAWLIALSLLATFLVSILLRKTF
                     LGKALRATIQDREAARLAGIPVERMSVIAFAVGGILIGLAGPLYGSTAYLHPWGGMEA
                     TLIAVVITIFAGVGRIRGVLLGGWALGLAESLAVVFLGTSWRELVSALVLIILLILRP
                     EGLLRVSYGPVR"
     gene            7206..8177
                     /locus_tag="PLM79_08390"
     CDS             7206..8177
                     /locus_tag="PLM79_08390"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV86367.1"
                     /translation="MMKKTVNPPVLLGTAFLLFFSLWPLTGVGTDLHCRVLGFAFYYG
                     ALALAWNLLALTGVISLGHAAFFGLGAYGSALLDHYWKLSPCITILAGGGLGTVYGIL
                     WHLVFKKLRGAPFALATLASVEVPKILIDNWDGLTLGSRGITGISDLPSLQWAGLSVL
                     LGRDPRALYYLFFALMLTVGWVHRRAFRSRWGWAIRSIREDETAAASLGVNVVRTRFQ
                     ALLLSAFFTALCGAIYPHLLGLVEPPLVFSLHLSALPLVLSIFGGRHQPYGPILGALV
                     LYPLDQLLFHSLLPVGHDAVYGLVVILTLLFFPQGIGAWLQTRLRSA"
     gene            8147..8938
                     /locus_tag="PLM79_08395"
     CDS             8147..8938
                     /locus_tag="PLM79_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86368.1"
                     /translation="MAPNKAQVCLELRHIEIGFGGVPLLKDIGLRVEEQEIVSIIGPN
                     GAGKTTLFNVICGHLHPDRGTVLFRGEDITRRPPHHICHRGIGRTFQIARPFPEMTPW
                     ENVMVGLSFGKRGRPGWASHGDEAMELLAMVQLEHKAQLPARELTLSELRRLEVARAL
                     ATRPDLLLLDEIAAGLSPQAVKQAVDLVKGLRSRGLTLLIIDHFLNLTAKVSDRLVAL
                     DQGEKIMEGTPVEVLQCPDVISAYLGESRQKVVKDDKIESHPESN"
     gene            8904..9695
                     /locus_tag="PLM79_08400"
     CDS             8904..9695
                     /locus_tag="PLM79_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86369.1"
                     /translation="MTRSSPTRNPTESESPPGPVLEVEDLAVSYRGTPVLESIRLRVW
                     PYQIVALVGPNGAGKTTLLKAIGGVVPLSAGRVLFCGQPVHLLPAHRVVQRGMIYIPE
                     GMRVFPQMSVMENLEIGAYLHRKSIPERLELVFGLFPELYEKRNSPSKDLSGGEQHML
                     TLARGLMARARLLLLDDPFMNLSPKIVRRFCDAFRLLSRRGVTLVIASQHVRRILHAA
                     DLAYLIEDGRITLSGPGLEVLGNVHLQHILLGSLPERIRQLDPLP"
     gene            complement(9770..10852)
                     /locus_tag="PLM79_08405"
     CDS             complement(9770..10852)
                     /locus_tag="PLM79_08405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOV86370.1"
                     /translation="MLPRFKSNEHVRSETEGCAREDRVRQTVTGRRFAVIHQGALGDF
                     LSALPLFDGLHESEPGIFIDFWSKAEYGSLLAAKPYLGAVHSRDDPALTPFYHEQLWM
                     DAPVPGTLAQAHVIFVFGQSNSRVLARRLAARCGVPVHWIQSFPDPHARRHVGRFLAE
                     QLHLLGWRCCTGIPRIDPDGEERRQVRRWLERWTGDAEAQPLVIHPGSGGMKKVWPLK
                     RWWSLVDWLLQRKVGPVVVILGPADDPLKTFARATRSRGCLLAEDLSLSRLAAFLAEA
                     RLYCGNDSGASHLAAAVGTPSVVVFGPTDPEVWAPRGPHVHVVQDCWKESEVLDWDEN
                     MPADDPPPAVTNLLDTLLGSPTQPRP"
     gene            complement(11258..11503)
                     /locus_tag="PLM79_08410"
     CDS             complement(11258..11503)
                     /locus_tag="PLM79_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOV86371.1"
                     /translation="MNDDGVRELVEVMAKALAEYPEAIVVKEIVGHQISVIELRAAKD
                     DLGKIIGKEGRNAHALRTILNAAATKLRKRAVLEILE"
     gene            11785..12546
                     /locus_tag="PLM79_08415"
     CDS             11785..12546
                     /locus_tag="PLM79_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86372.1"
                     /translation="MDSETVAEWFRNPPEPLSESLSRYQRKMADYLLEMGRLDGLENL
                     ERSGTLPGAALKYFQRVLDLYDEAVQLFLGYLRRSGMDVQCTAGCSHCCYHMPTGVSS
                     MELIYLYYGMQRSGSSGRLFRRCLEAEEVWFGIYRQVGADPSTPDPRSALLTRYHQEQ
                     RPCPFLEEGRCLVYPYRPFACRMHFSLSPRYWCHPRRAQNSETVQFNVEPGPCVLDAL
                     DRLEDRMQLAASDVLVCGMLELTVNILHFEPLVWS"
     gene            12569..14185
                     /locus_tag="PLM79_08420"
     CDS             12569..14185
                     /locus_tag="PLM79_08420"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOV86373.1"
                     /translation="MTLELPDWMEERPWVETVSLELPLPGLHDNTADWDRLERALEQG
                     GFSPVLVSPRCLSGFIASLRAERFRVSAVLGQTPWGWELIEVGPYEHDAPVLGLAVDL
                     GTSQLVFYLVDLRSRRLLSRISAPNPQIPLGEDILQRILFARHQENRTTLQRSLITAF
                     NRSVGQMIEERDLPPSRVFAVAVCGNPAMSHFFLGLDPSNLCKEPYIPSANRFPLHHA
                     ADLDLDIHPRAPVYVFPNVGSYFGGDLLAGILAAGIHRSEDLSILVDVGTNAEVVLGN
                     RDWLIACAGAAGPALEGGVVERGMMASPGAIDRVRIDGRTWEPSFHVLGDGKPRGICG
                     SGLIDLVAEMFRAGILTIQGKINVQAGSPRVIVTDDGPAYIVAHREETEDGRDLVVTE
                     VDVGIFLKSKAAMYTILNVITRKVGVTFQDLKHFYVAGAFGNRIDPEMAIRIGMLPDL
                     PPDTFHGLGNTAGKGAVMLLLDRSLFGRIEEIRKRITYVELNVNLELMNEFRGALFLP
                     HTDPRLFPSVRIPQRDCRTDESGPFHGALP"
     gene            14182..14541
                     /locus_tag="PLM79_08425"
     CDS             14182..14541
                     /locus_tag="PLM79_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOV86374.1"
                     /translation="MRSNHHPDIKIRRDERPFLSGVQVLLCLSGILLVLNLVLLYGIF
                     FSPQGIFGFRQQYQQIVAMESRIFNLKSENQKLFRKIQSLKSDPQAQERLVRDHLGWV
                     KPNEVMVEFPPPRKDPR"
     gene            14707..15132
                     /gene="rpsF"
                     /locus_tag="PLM79_08430"
     CDS             14707..15132
                     /gene="rpsF"
                     /locus_tag="PLM79_08430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOV86375.1"
                     /translation="MRKYETFFIVDPDLPDEVHSVIDDKLQSIVQSNGGMVLSYVPWG
                     KRKLAYPIKKRSRGLYVLMEYAGGSKLVAELERNLRLDERIIKFLTVKLDDKFDPAAL
                     EAAEAPAREAVAEVEEAPTSPPPEEGAEEAEERTPEPLE"
     gene            15177..15422
                     /gene="rpsR"
                     /locus_tag="PLM79_08435"
     CDS             15177..15422
                     /gene="rpsR"
                     /locus_tag="PLM79_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOV86376.1"
                     /translation="MAIRPKRRTFHRRKVCRFCMDGELKIDYKDSKTLRYFVTERGKI
                     VPRRISGNCAKHQRELTLAIKRARQIALLPYTTIHTI"
     gene            15571..16545
                     /locus_tag="PLM79_08440"
     CDS             15571..16545
                     /locus_tag="PLM79_08440"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HOV86377.1"
                     /translation="MAIGNGASPGRVGQRGKEVLLGIGGTLAFFFSAIFVPMVGLFAG
                     IFTPFPTLLFFYLWGPPWGYAIPGGAALVGTLVLAVLNLGQNAPYLLEMLVFGLFLGF
                     AMRRRWPVERVVAASTALVTAIGAFLLWTSLHGTEAGFLKALEEDFGNSVTSTLQQYG
                     ALSKDHGEMNDAVQRVIPVMVRLFPAIAVSSAILISWVNLLVTRRYCRNRGVSLPPWG
                     EWSHWKAGESLVWGVIAAGFSLLLPVKMLQLVGLNVLMVMGTIYLLQGFSILAFYFER
                     WNLPRALRAFLYALLFVQQYLALGAVLLGLFDVWADFRRLSKKPVEPA"
     gene            16695..17141
                     /gene="rplI"
                     /locus_tag="PLM79_08445"
     CDS             16695..17141
                     /gene="rplI"
                     /locus_tag="PLM79_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOV86378.1"
                     /translation="MKVILTENIASLGEIGDVVNVAPGYARNFLLPQKMAMEATGRNV
                     RELDHKKRMLAQKREKIRQEMLSLAEKLNQVKITLRRKVSEEDKLYGSVNSSDLTNVL
                     QEMGFDLPRKSISLEHPIKELGEYSVPVRVDAQITATISVTVEKEE"
     gene            17147..18487
                     /gene="dnaB"
                     /locus_tag="PLM79_08450"
     CDS             17147..18487
                     /gene="dnaB"
                     /locus_tag="PLM79_08450"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOV86379.1"
                     /translation="MDAVAEELKKVPPQQIEAEQSLLGGLLIDNDGLPAAFEVLRGDE
                     FYRDGHRLIFRAIQELFDRNEPVDLVTLADHLGEKQQLEQVGGTSYLASLIEAVPSAA
                     NVPVYAKIINEKALLRRLIQAAGEIISHCYGGGKSVEEILDDAESAIFSVTDNRIRSS
                     YFLIKDIVKKSIEAIERYQEHREMVSGVPSGFKDLDKLTAGFQPSDLIIIAGRPSMGK
                     TALALNIARNVSVQNKIPVGFFSLEMSKEQLAMRLLCMEGMVDSHKIRTGFLSRQECA
                     KLLTAAGAFMDVPLFIDDTPAITSLELRAKARRMMADKGLGVVVVDYLQLMRSREGAE
                     RREQEISEISRSLKALAKELNVPVIALSQLNRKVEERNNKRPILSDLRESGAIEQDAD
                     VIAFIYRDEVYNPDSADKNIAEVSIGKQRNGPIGTIKLHYINSYTRFENLAHGI"
     gene            18840..19682
                     /locus_tag="PLM79_08455"
     CDS             18840..19682
                     /locus_tag="PLM79_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOV86380.1"
                     /translation="MLLIGWDGVQYNHLQEMLSAGELPNLSRFPVLVRTEITDHATST
                     KPGWVQILTGLEASVSGTRSNGIYRPFPQRACLFDILKKDAPGRNLWTAFVAGKDHHL
                     GSMGPGVRWIDGDGKPTSSRDGEPWFHARKSFDLWMGDMDRDADEVGTLAISLLDRFA
                     KRNQFAVFVHFADPDCKGHRFGENSPEYSQAVSDCDAWLGRILGKLDELGVAGKTLVA
                     VVTDHGFDEGEFRHHRSYDAWLAVRTHRNPRWHDGDQKDVAPTLLRIMGSGTEGMTGK
                     PLWW"
     gene            19722..19961
                     /locus_tag="PLM79_08460"
     CDS             19722..19961
                     /locus_tag="PLM79_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOV86381.1"
                     /translation="MEERNNKRPILSDLRESGAIEQDADVIAFLYRDEVYNPDSADKN
                     IAEVSIGKQRNGPIGTIKLHYINSYTRFENLAHGI"
     gene            20127..21452
                     /locus_tag="PLM79_08465"
     CDS             20127..21452
                     /locus_tag="PLM79_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOV86382.1"
                     /translation="MKRTDQYWDASVEALSRSQLDQLQLRKLKQVLDRVQSSVLYGPR
                     LKEAGLSSAGLSAIEDIRRIPFTTKADLRSQYPFGLVCVPKDRLVRLHVSSGTTGQAT
                     AVLYTRGDVDRWADLLARCMYMTGARPGHTFQNLTGYGMFTGGLGFHYGSERLGLLTI
                     PSGAGNSKRQIQLMRDFGTNILHIIPSYALRLMDVLGEMGMDPKHDLELKIAYLGAEP
                     YSEEVRRRVEDFYGIKVYNSYGLSEMNGPGVAFECPFQSGMHIWEDAYLVEIVDPETL
                     EPVEDGTFGELVLTTLDREAMPILRYRTRDVTRILPGDCPCGRVHRRLDRIRGRSDDM
                     FIIKGVNIFPIQVEHVLMNIPEVGNNYVIVLRRENDIDEMLVRVEVTQELFVEDMRHL
                     HRVRKEIVRNLKNELLVTPAVELVEPNSLPKSEGKAVRLLDQRTPRKGG"
     gene            21547..23166
                     /gene="hflX"
                     /locus_tag="PLM79_08470"
     CDS             21547..23166
                     /gene="hflX"
                     /locus_tag="PLM79_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOV86383.1"
                     /translation="MISPDLARDLARISYECGRQIGLLVARSGTVEMVLAGGPGSLYI
                     PDLPKSRGGRSHLRGLRLVHTHLGEEPLSQDDLMDLVFLRLDCVAAVCVDRHGGATRL
                     EVAHILPSSRDREPAWRILPPLPCYDPDLDIRQMVQSLEEELERNRLAVEASRAEDRA
                     ILVSVTSADKETALDSMAELQELARTGGIEAADTIIQRQREVNPKYLIGKGKLSEIVL
                     RALQCGVDLLIFDQELNPSQVKAITDTTELRVIDRTQLILDIFARRAHSREGKIQVEM
                     AQLKYLLPRLGMKDDALSRLTGGIGARGPGETTLEINRRRTKDRIVHLETELAAVRKN
                     REQRRARRTRKDVPVISIVGYTNAGKSTLLNTLTHSGVFVENKLFATLDPTSRRLRFP
                     RDIEVIITDTVGFIRDLPKDLMEAFGATLEELSDADLLLHVVDLSNPQFENQIRAVDR
                     ILADLNLRQKPTLLVFNKMDRADPALVERVLRRHGGVAVSALSTKTLEPLILAMEEQI
                     TAQLEEGLFPRALGETEEGGSDPWTARSPLP"
     gene            complement(23275..24216)
                     /locus_tag="PLM79_08475"
     CDS             complement(23275..24216)
                     /locus_tag="PLM79_08475"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOV86384.1"
                     /translation="MEVFEGIDPIPRSLKNPVLTIGNFDGVHRGHQAIFNRVKAWAAQ
                     INGESVVLTFYPHPLRVLNPNEELSFITSHQRKIELMADSSIHVAIVIPFSREFALIS
                     ARDFVKNILVDRIGVKALVVGYDYRFGYGREGNIRFLRELGDQYGFGVEAVEGIELEG
                     TMVSSTVIRHLVSEGNLRTANKLLGRSYEISGTVIHGRQRGARLLGFPTANIKAEAWQ
                     VTPKPGVYAVQVVVDGKLYGGAGNLGYNPTFNDRELSLEVHIFDFNDTIYDKPITVKF
                     VDRLREERRFSGPEELAVQIRKDIERSRQILAEHPGF"
     gene            24317..24403
                     /locus_tag="PLM79_08480"
     tRNA            24317..24403
                     /locus_tag="PLM79_08480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24351..24353,aa:Leu,seq:cag)
     gene            complement(24454..>24621)
                     /locus_tag="PLM79_08485"
     CDS             complement(24454..>24621)
                     /locus_tag="PLM79_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV86385.1"
                     /translation="SAVVEGINNQIKTLKRQAYGFRDHEDFKLRLYPLHAQRYSLAGW
                     ADLFFCVTHCG"
BASE COUNT         4891 a         7157 c         7465 g         5108 t
ORIGIN      
        1 gtaggattcc ttgaggtcga tgtatttgcc gacgatggcg atgcggacct cgtgggaggg
       61 cttcttcatc ctttgaacca gctgatccca ttggtccagg acgggagccc gggtccagat
      121 gttgaggagc cgaacgatct tttcgtccag gccttccttg tgaaagagga gggggacttc
      181 gtagatgcag tccacgtcct gggcggtgat caccgcatcg ggttccacgt tgcagaagtg
      241 ggcgatcttc gccttgaggt cgcctggaag aagtcgctcc gttcggcaca aaaggatgtc
      301 gggctggatc ccgattccca gcagctcttt gacgctgtgc tgggtgggct tggtcttgag
      361 ctccccggag gtcttcatga agggaaccag ggtcaaatgg atgtacagga cgttttcttt
      421 tcccacatcg tttcgaaact ggcggatggc ttccagaaag ggcaggcttt cgatgtcccc
      481 gacggtcccg ccgatttcga cgatggcaac atccacgccg ttgacggcac cccggatgca
      541 gctcttgatt tggtccgtca cgtgggggat gacctgcacg gttcccccca ggtagtcgcc
      601 ccgcctctcc ttggtgatga cggaatagta gatgctgccg gaagtgtagt tgttgttctt
      661 ggacatgcgc gcatgggtga atcgctcgta gtgtcccaga tccaggtccg tttccgcgcc
      721 gtcgtcggtc acgaagacct ccccgtgctg gaaggggttc atggtgccgg gatcgacgtt
      781 gatgtaaggg tcgagcttct gcaacgtcac cttcagacct cggctctcca tcagggcgcc
      841 gatggaagcg gccgcaaggc cttttcccag ggaagagagc accccacccg taatgaagat
      901 gaattttggc tggcgcaagt ccgctccttt caggctaaaa gtctaagaaa atgccctatt
      961 ttggaacggg aagcagtata gcaaacggga gtccaaagtc aatgtcggcc caggggtccc
     1021 tccggggagt cggaggaggg gacgggtccc ggaaaaggaa tgccgcccgt ggaggggaaa
     1081 gggccgaggg gaagcgatta ggcatacacg atccctgggg tccccgcttg tggtaatttt
     1141 cccttgcaat ttttgtcaat ttgacgtagg tatcaggagg tcgataggag aacttcctgc
     1201 aatccctaca gaattcgcaa cgtcgtcttc ggctttttgg cagcgtccgt cattgagggc
     1261 ccgcaccatg attgtcggcc agtggatgac caccaaaatt gttaccatcc ggaaggagtc
     1321 ttccatccag gaggccctgg cggtaatgaa gagggagtcc atccgccatc tgcccgtgac
     1381 ggacgaagac gggaacctct tgggatgggt tacggatgcg gaccttcggg gagtgctcat
     1441 cgcttccatg ctggaagagc tgaccctgga agacgtcatg gtgcgcaagc cttacgtagc
     1501 cggtcccgcg atgccgctgg aagaagcggc ccgcatcatc ttggaaaaga agatcggggg
     1561 tttgcccgtg gtggcggacg gcaagctggt cggcatcatc accgtagtgg atattttaac
     1621 ggcattcatc accatgatcg ggatgttgag ccagtcgtcc cgcctggatg tgcgaatcac
     1681 gaaaccgggc acccccctgg atgagatcac ccggttgatc cggcatcacc gggcggaagt
     1741 catcagcatc tgtcacctgc cgggccagga acgtcaggac ccaatctact cggttcgtct
     1801 gaagaagtgc gatctccagc ccattgtttc cgacctcctg gacaatggaa tagaagtggt
     1861 gtcctcgata ccgtagcaga tcctcggatt ggttgccgct ctcagcagga acgtttcgaa
     1921 gtccgatcca ttctgattct tcctgattcc ttccttctac tgtgcttccg cgtctccgga
     1981 atcctcgagg gattcgcgtc gactgcaccg cgtcggcacc ttttgcgatg aagaaacagc
     2041 gctcgggccg ggtccgggtt tgcccggagc aggccattcc cccgtcgggg gcggttgcgt
     2101 gcatccggac ggcccgagag cgcatgaaga ttgagtggaa aggaggtggt ccgctccggc
     2161 agccgtcgct cggcatcaaa ggagggaagg gagccgagat cccgtggggc atcggctcgg
     2221 aaacgttggt tcctgtttga gacaaggagg aattcaagga tgggaaaaat tccagaaaac
     2281 tacctgccgc ccaaagaaac ctggccggtg tatttgaccc cggatgaatt tgccgacatt
     2341 ccccaccagc tgaaccttgc agactttctc ctggaccgtc atgtgcggga aggtcgaggg
     2401 gagaatcctg cgatcaagtt catggataag accattacgt acggtcaact gcagcaaatg
     2461 gtcaaccgat tcggcaacgc gctcaaagcc gtcggggtcg aaccgcagga tcgagtgggg
     2521 attcgtttgg tgaattcccc ccaagccctt gtgacgatct tcgccatcga aaagattgga
     2581 gccgttcccg taccgacctc ccctctgtgg tccgccgaag aagtgggctt tgtggccaac
     2641 aacgccgaaa tgaagttttt cgtggtcaac gcccccctca tggaaccggt gaccaaggca
     2701 aagccggaat tcgagtatgg aacccggatc atcgtcatcg gtggaaaccc gaacgaggtc
     2761 aaggccgccg gggacctggt gtacgaggaa atggtggaaa aaggctctcc ggacctggaa
     2821 gccaccatgc tgaatgccga cgatatcggg attatgctgt acacttccgg gaccacgggc
     2881 atgcccaagg gttgcgtcca ttttgtgcgg ccggccgtca tggtttctcg agtggtcaat
     2941 cgttatgtct accagatgaa gccgggggat atcctgggag gcgccgcgcc ggtgtctttt
     3001 gcggccgggt tcggcacttt cagcctcatc cccttcgaag ggggtgcggc ggtctccctg
     3061 attcctagat tcgctccccc ggacatgatg gacctcatcc agaaacacag gatcacgatc
     3121 ctcacgggcc tgcccacggc ttatcgtgcc ttgatgaagt tcccggactt caagaaatac
     3181 gacatcggct ccgttcgcct ctacacctcc gggggagacg ccctgggtgc cgagaccctg
     3241 caggcctggc aggaattgag cggcaagccc atctgggaag gactgggcgg cacggaaatg
     3301 ctccatctgg tgacctcgaa caccatgaac cccgagccgg tgccgaactc catcgggaaa
     3361 ccgctccccg gggtcaaggt gaaggtggtg aacgccgaga ggcaagaatg caagccggga
     3421 gaaatcggca gcatgattct ccagggacct tcggggaccc tgtactggaa gccttacgag
     3481 gaggacgaga agcttctccg atcccagaaa aaaggggtgg tggacggctg gaaccagatg
     3541 ggagacgccg tctacatgaa cgaagacggg aatatcttct ttgtctcccg ggaagacgac
     3601 atgatcaaga gttccggata ccgcatcggt cctgcggaag tggaggaagc catcctgaag
     3661 catccggcgg tggccgatgc aggcgtggtg ggcattcccg atccggacaa ggggcagatc
     3721 accaaggcgg tggtggtcct caagcccgga taccaggggg gagacgcgtt cttcgaggag
     3781 ctcaaagagt tcctcaagca gtacatcgcc atctacaagc ttcccagggt gatccagtac
     3841 atggatgctc tcccccgcac cccaaccggc aagctgctcc gcaggaagct tcgatagatc
     3901 gttttttcgg gaaggcacct gcccccccgt cgacctcata cccccgcgct caggcggggg
     3961 tatgcatttg agaggacgcc atggattgga agattaccgt cttgtgtgag aatacggtgg
     4021 caagccccgg gttcttgggg gagcatggct ttgccgccct ggtcgagacc cccgaggaga
     4081 ccatcctttt cgatacggga caaggatttg ggctggttca gaatgccctg aggctccaga
     4141 aggatttgcg ccgggtcacc cggttggtcc tcagtcacgg ccatttcgac cacacgggag
     4201 gcctgctctc gtttctgggc atccatggcc cgtgcgacgt gacggctcat ccggatgtgc
     4261 tcgtcgaacg gtttcggtgg atgcccgtcg ggtccgagga aaagcccttg tccatcggaa
     4321 ttccgtggca cgaggcctac ctgacgaccc ggggggcaag gttccggtgg cagagggagt
     4381 ttgcggagat ttcccccaac gttttcatct ccggcgaggt cccgcgcaag accgcctttg
     4441 aaacggggga ccccaagttt gtcgttcgaa agaacgggca gtgggccccg gacccgtttc
     4501 tggacgacta ctcccttttc gtcgtcacgc cccggggcct cattgtcctt ctcgggtgtg
     4561 cccacgccgg gctcatcaac atcttgaacc acgccgtcga gaagacgggc gaagagagaa
     4621 tccacgccat cgtgggcggg actcatctgg ggttttcccc cgaggttcaa accgaggaca
     4681 cccttcgggc gctgaggaaa taccggatcg aggtcctggc aaccagccac tgtacgggac
     4741 agcgtcccgt cgcccgcctg gctgccgagt tcggggaccg cttcgccttc gggcacgtgg
     4801 ggtttgtctt gggcgtgttt tcgggctgag tcgggaccat atgcgttgca taaattgcgc
     4861 tgctgttata tgtcaatgac acaggtgggg ttggaaaagc ggctgcaatc gagaagggga
     4921 ccctttgggt cggcaggcat cctatgcaac gattccgagc tgggttgggg attcttctat
     4981 ggttgtttct cttcggattt cacgccgagg ccttctcgat tcggatcggg gaaatcaacc
     5041 ccctcacggg aaagctggcg gaacacggca ccgacatcca cgagggaatc ctgtttgcgg
     5101 tggaagaaat caatgcccgg ggggggatcc acgggagccc ggtggaattg atcagccgtg
     5161 atgaccaaag ccagccggaa gtggccgtca accaggcgga ggagctgctc taccgatccc
     5221 gggtggtggg tctggtgggg ggatacgtgg attccctggt ggggcccatc agcgaactgg
     5281 ccgccaagca ccgggtccct tacgtggcgg cggccagcct tcaacgatcc ctgaccctga
     5341 ggcgctccaa tccgtttttc ttccgcgtgt ctcagcagaa gggcattgtt cagcccttgt
     5401 gcgactttat cctggagcaa gcccgagccg ggcgggtggc cgtcctgtac gccgcgacgc
     5461 cgggttcctc ggaatttgga gaagacgtaa agggtcttct ggaaaaaaga ggggtccagg
     5521 tccctctttt tgagaagttt cgtccgggtt ccccggattt ctcctccttt ctgctcaaga
     5581 ttcggcagca taaaccggac gttctcgttt ccggggggtt cttccccgac catcttgtcc
     5641 tgatccgtca gtcgagggag cagcaggttc ccttccgatt ttatatcggc ccgtggggga
     5701 tagcccatcc gtcgttcatc cgggaaatgg gttccatgag cgaggatctg ttggggatgt
     5761 gcgcatggaa cccggggatt acgcttccgg gaacggaggg agagtccgcg cggtttgtgg
     5821 aggggttttc ccgtcgtttt gggaaggtgc ccaattcgac caccatgcac ggctatacgt
     5881 cggctcgggc cctgttggcg gcaatggaag gtgctgccca aaagggagtg tcctggacgg
     5941 gcgaggccat ttcccgggag ctccgatccc tggacatgct gcttcccatg gagcggctgg
     6001 ccttcgacga gaacggcgat ccccggtact atcaacaggt gattgttcaa atccgaaacg
     6061 ggcaaatggt gccggttttt ccccaagacc gcgccgcggc aaccgtccgt ttccctcggg
     6121 actaaagggc cgtccaacaa cgctctcttc accctgcctt cgcccctatt acccgaggag
     6181 ggagaaaggt tccttccgcc cgggggggag acgggggtga agagaattcc atcggcggat
     6241 gggtccggac cgaaacgaaa cgcatgaatc gctacgagca gggttgactc gaaccgggga
     6301 ctcttactca ttcaagttcg gcatgaggcg ggaatcatcg tcatgtggct gcaactggcc
     6361 ctcaacgcct tcactctggg ctcgttttat gccttgatcg ccctgggctt ttccctgatt
     6421 ttcgggatca cccgggcctt caacctggca cacggggaaa tggtcctcct gggcgggtac
     6481 ctggcttacg tcttgggaac ctactggcaa gtcccgtttt tctggactct tcccgcgtgc
     6541 atgttgactc tccttcttct tgccgtactt ctccaggcac tgctccagca tgttcgggaa
     6601 cccttcgagc tcaattccct ggtggtgacc ttcggactgg ccctggtcct gcaaaacggg
     6661 atgcttgtcc tgttttcggc caaccatcgt ttgatccgga atgcagaggc ggcatggagc
     6721 cttccgtccc tgcatctgac cctaactcag ctccaggcat ggctgatcgc actgtccttg
     6781 ctggcgacct tccttgtctc catcttgttg cgcaagacct ttctggggaa agctctgcgt
     6841 gccaccatcc aggaccggga agcggcgagg cttgcaggaa ttcccgtgga gcgcatgagc
     6901 gtgatcgcct tcgccgtcgg aggaatcctg atcggccttg ccgggcccct ttacggcagc
     6961 acggcctacc tgcatccctg ggggggaatg gaggccaccc tcattgccgt tgtgatcacc
     7021 attttcgcgg gggtcggacg catccggggg gtgttgctgg gcggctgggc cctggggctg
     7081 gccgagtccc tggcggtggt cttcctggga accagctggc gtgaactcgt gagcgccctg
     7141 gtcctgatca tccttctcat cctgcggccc gaggggcttc ttcgggtgag ctacgggccg
     7201 gtgagatgat gaaaaagact gtgaaccccc cggtcctgtt gggcaccgcc ttcctgcttt
     7261 tcttttccct gtggcctttg acgggggttg gaacggacct gcactgccgc gtgctgggat
     7321 tcgccttcta ttacggtgcc ctggccctcg cctggaacct cctggccctc acgggggtca
     7381 tttccctggg acatgccgct tttttcggct tgggcgctta cggatcggct ttgctggacc
     7441 attattggaa gctttccccc tgcatcacga ttcttgccgg cggaggcctg ggcactgtct
     7501 acggcatcct ttggcacttg gtcttcaaga agttgcgggg ggctcccttt gccctggcca
     7561 ccctggcgtc ggtagaggtc cccaagatcc tcatcgacaa ctgggacggt cttaccctgg
     7621 ggtcccgagg aatcaccggc atatccgacc ttccatccct gcagtgggca ggactgtcgg
     7681 ttcttttggg gcgagatccc cgggcattgt attacctttt cttcgcgctg atgcttaccg
     7741 tgggctgggt ccaccggagg gccttccggt cgagatgggg gtgggccatt cgatccattc
     7801 gcgaagacga gaccgcggcg gcgagcctgg gggtgaacgt ggtccgcacg cgatttcaag
     7861 ccctcctcct cagcgccttc tttacggccc tttgcggggc gatctaccct catttgctcg
     7921 gtctggtgga acctcccctg gtgttcagtc ttcacctgtc cgccctgcct ttggtgttga
     7981 gcattttcgg aggcaggcac cagccctacg gcccgatcct gggagccctg gtcctgtatc
     8041 ccctggacca gctcctgttt cactccctgt tgcccgtggg gcacgacgcg gtctacggtc
     8101 tcgtggtgat cctcaccttg ttgtttttcc cccaaggaat cggtgcatgg ctccaaacaa
     8161 ggctcaggtc tgcctagagt tgcgacacat cgagatcggg ttcgggggcg tccccctcct
     8221 caaggacatc ggcctcaggg tcgaagagca ggagattgtc agcatcatcg gcccgaacgg
     8281 tgcgggcaag accacgttat tcaacgtcat ctgcgggcat ttgcatcccg atcgaggcac
     8341 cgtcctcttt cgcggagaag acatcacccg ccggcctccc catcacatct gccacagggg
     8401 aatcggtcga acctttcaga tcgcgaggcc ctttcccgaa atgaccccct gggagaacgt
     8461 catggtgggg ctcagcttcg gcaaaagggg tcgcccgggg tgggcttccc acggggacga
     8521 agccatggaa ctgctggcaa tggtccaact ggagcacaag gcgcaactcc cggcccggga
     8581 gctgaccttg tccgagttgc gaaggttgga ggtggcccgt gccctggcca cccggccgga
     8641 cttgcttctg ctggatgaaa tcgccgccgg attgagtcct caagccgtga aacaggccgt
     8701 ggatctcgtc aagggactcc gaagccgggg tctcaccctc ctcatcatcg atcatttcct
     8761 gaatcttacg gcgaaggtgt cggaccggct cgtggccttg gaccaggggg agaagatcat
     8821 ggaaggaact cccgtcgagg ttctccagtg tcccgacgtc atatcggctt acctgggaga
     8881 gagcaggcag aaggtcgtca aggatgacaa gatcgagtcc cacccggaat ccaactgaat
     8941 cggagtcccc gccgggtccg gtcctggagg tggaagacct ggcggtttcc tatcgcggga
     9001 ctcccgtcct ggagagcatc cgtctgaggg tttggcccta tcagatcgtg gccctggtgg
     9061 gacccaacgg tgcaggcaaa accacattgc tcaaagccat agggggggtg gtgccgttga
     9121 gcgccggtcg ggtcctgttt tgcgggcagc ccgttcattt gcttccggcg caccgggtgg
     9181 tgcagagggg catgatttac atccccgaag ggatgagggt gtttcctcag atgagcgtca
     9241 tggagaacct cgaaatcggg gcctaccttc atcgcaaatc catcccggaa cgcctggaac
     9301 tggtgttcgg cctctttccg gaactttacg aaaaacgcaa ttccccatcc aaggacctga
     9361 gcggggggga gcaacacatg ctcaccctgg cccggggcct catggcccga gcccgcctcc
     9421 ttcttctgga cgatcccttc atgaatctca gccccaagat cgttcgaagg ttctgcgatg
     9481 ccttccgact cctgagccgg cggggcgtga ccctcgtcat cgccagtcag cacgtgcgaa
     9541 gaattctgca tgccgcagac ctggcgtacc tcatcgagga cggccgcatc accctgtcgg
     9601 ggccgggtct cgaggtgctg ggaaacgtcc acctgcagca catcctctta gggtcccttc
     9661 cggagaggat ccggcagctg gatcccctcc cctgagcgac cgttcggact ccccagggag
     9721 ggtcctgaga tgatcaggac ccggcgaggc cccgcattcc tgtccatgtc tatgggcgcg
     9781 gttgcgttgg ggacccgagc agggtgtcga ggaggtttgt cacggcaggg ggcggatcgt
     9841 cggcgggcat gttttcgtcc caatccagga cctcggactc cttccaacag tcttgcacca
     9901 catgaacatg agggcctcgg ggggcccaca cctccgggtc cgtgggaccg aagaccacca
     9961 cggaaggtgt gcccaccgca gccgccaggt ggctggctcc ggaatcgttt ccgcaataca
    10021 ggcgggcttc ggccaggaat gccgccaggc gggaaagaga aagatcttcg gcgagcaggc
    10081 atccccggga tcgggtcgcc cgggcaaagg ttttcagcgg gtcgtcggca ggcccgagga
    10141 tcacgacgac gggtccgact tttctttgga ggagccaatc cacaagggac caccagcgct
    10201 tcaacggcca gaccttcttc attccaccgc ttccggggtg aatcaccaag ggctgtgcct
    10261 cggcgtctcc ggtccaccgc tccagccaac ggcgtacctg tcgtctttct tccccatcgg
    10321 gatcgatgcg cgggattccc gtgcagcacc gccagcccag aagatgcagt tgttctgcga
    10381 gaaaccgccc cacgtgacgg cgggcgtggg ggtccggaaa cgattgaatc cagtggacgg
    10441 ggaccccgca gcgggcggcc agcctccggg caagaacccg gctgttcgac tgcccgaaaa
    10501 cgaagatgac atgggcctgg gcgagagtgc cgggaaccgg ggcgtccatc caaagctgct
    10561 cgtggtagaa aggggtcagg gcggggtcgt cccgggaatg aacggcgccc aggtagggct
    10621 tggctgcaag caaagacccg tattctgctt tggaccaaaa atcgatgaag atccccggtt
    10681 ccgattcatg gagaccgtcg aacaagggca gggccgacag aaaatccccc aaggctcctt
    10741 ggtgaatgac cgcaaaccgt cgtccggtaa cggtttggcg gacccggtcc tcccgggcgc
    10801 acccctcggt ctcggaccgt acgtgctcgt tggacttgaa acgaggcaac atcctcagtt
    10861 tgtcccggac atgggcaaaa cgtcgaatac ggaaagagcg ggacaagtca ttcttgccct
    10921 gggagggaag ctcccgcttg ggtctgttcc ttggccagtt tctggtcgga ccatcgtttc
    10981 agaaaacccg tgactcggga aaccacagga gggggagaag agggtttcgg cagcacgccc
    11041 tgtttttgaa gggcttgccg cagacatcct ttcaggtgtt cgcaggacaa acaatgggct
    11101 tggggctgga tgattccgtc ttcatctctg ggacacactt gacgggggtc cccaaaacag
    11161 gttggaggac cctcatgagg gggaacgaac cggctcttgt ctttttgatg aaggctcatg
    11221 gcacatggac gggaggcggg ctcccttctc cccggcatta ttccagtatt tccaggactg
    11281 cacgtttccg aagcttggtg gccgctgcgt tgaggatcgt ccgcagggcg tgagcgttgc
    11341 ggccttcctt cccgatgatc ttacccagat catctttggc cgcccgaagc tcgatcaccg
    11401 aaatctgatg acccacgatt tccttcacca caatcgcttc cgggtattcc gccaaagctt
    11461 ttgccattac ctctacgagt tcccgcactc catcgtcatt cataaatttt ctctactccc
    11521 tagtcgcaaa cttacgttcc atgccggtgg gattcccaaa aatgccttcc cttttgacac
    11581 tcgattcgct accttgagca ttagccaaat gattctattc gagttgagcc gaacatgcaa
    11641 cagattgctg acgggcggcc cccgccatct cccggcggtt acgtgaaaaa accgtacaat
    11701 gtgcggcgga atgccgacgc aggcggggaa agtgcgccaa gaggaccaag aagggcggca
    11761 gtcgttgaat ttcaaccgag aaacgtggat tccgagactg ttgccgagtg gttccggaat
    11821 ccgccggagc cgctttccga gagtctttcc cgctatcaaa gaaagatggc ggactatctt
    11881 ctcgagatgg gtcgcctgga cggcctcgag aacctggaac ggtccgggac tctgcccggg
    11941 gcggcgttga agtattttca acgagttctg gatttgtacg atgaggcggt ccaactgttc
    12001 ctggggtacc ttcgtcgctc ggggatggac gttcagtgca cggcgggctg ttcccattgc
    12061 tgctatcata tgcccacggg cgtcagttcc atggagctga tctatctcta ttacggcatg
    12121 caacggagcg ggagttccgg ccgcctgttt cggcggtgtt tggaagccga agaggtgtgg
    12181 ttcggaattt accggcaggt cggagccgat ccctcgaccc cggaccccag atcggccctt
    12241 ttgacccgat atcaccaaga acaacgccct tgccccttcc tggaggaggg gcgttgcctc
    12301 gtttacccgt atcgtccttt tgcctgccga atgcacttca gcctgtcccc ccggtactgg
    12361 tgtcatcccc gtcgggcgca gaattccgag accgttcaat tcaatgtgga accgggaccc
    12421 tgtgttctcg acgccctgga caggctcgag gaccgaatgc aattggctgc ctcggacgtc
    12481 ttggtgtgcg ggatgctgga actgaccgtc aatatcctgc acttcgaacc cctggtctgg
    12541 tcgtgaacgg gaggatcaaa aaggttccat gacccttgag ctgcctgatt ggatggaaga
    12601 acggccctgg gtggagacgg tttccctgga actgcccctt cccgggttgc acgacaatac
    12661 ggcggactgg gaccggctgg agagggcact ggaacagggg ggcttttccc cggtccttgt
    12721 gtcgccccgt tgtctgtccg gattcattgc ttccctgcgg gccgaacgtt ttcgagtctc
    12781 tgcggttctg gggcaaaccc cctggggctg ggagttgatc gaggttgggc cctacgaaca
    12841 cgatgctccc gtcttgggcc tggccgtgga cctggggacc tcgcagctgg tcttctacct
    12901 ggtggacctt cgttctcgcc gcctcttgag ccggatttcg gctcccaatc cccaaatccc
    12961 cctgggagaa gacatcctcc aaaggatcct gtttgcccgg caccaggaga accgaacgac
    13021 tctgcaacgc tctctgatca cggccttcaa ccggtccgtc gggcagatga tcgaggaacg
    13081 ggaccttccc ccgtcccggg tgtttgccgt agccgtgtgc ggcaacccgg ccatgagtca
    13141 tttcttcctg gggctggacc cgtccaatct gtgcaaggaa ccctacattc cttcggcaaa
    13201 ccgttttccc ctgcatcacg ccgccgatct cgacctggac atccatcccc gggccccggt
    13261 gtatgttttt cccaacgtgg ggtcctactt tggaggagat ctccttgcgg ggatcctggc
    13321 cgcggggatt caccgttccg aggatttgag cattctggtg gacgtgggca ccaatgccga
    13381 ggtggtcctg gggaatcgcg actggctcat tgcgtgtgcc ggggcggcgg gaccggcttt
    13441 ggaaggcggg gttgtggaac gcggcatgat ggcttccccg ggggccatcg atcgggtccg
    13501 catcgatggg cgcacatggg agccttcctt tcacgtcctc ggggacggaa agccccgggg
    13561 gatctgcggg tcgggcctca ttgacctggt ggcggaaatg ttccgggcgg gcatcctcac
    13621 catccaggga aaaatcaatg tacaggccgg gtcgccccgt gttatcgtaa ccgacgacgg
    13681 cccggcttac atcgtggcgc accgagagga aaccgaggac ggccgggacc tggtcgtcac
    13741 cgaggtggac gtggggatct ttctcaagtc caaggccgcc atgtacacga ttctcaacgt
    13801 gatcacccgc aaagtcggcg tcacttttca ggacctcaag catttttacg ttgccggggc
    13861 gttcggcaac cgcatcgacc cggaaatggc gatacgaatc gggatgcttc cggacttgcc
    13921 cccggatacc ttccacggct tgggaaatac ggcgggaaaa ggggcggtca tgttgctcct
    13981 ggaccgctct ctgttcggcc ggatcgagga aattcgaaaa cgaatcacgt atgtggagct
    14041 gaacgtgaac ctggagctga tgaacgagtt ccgcggagcg ctcttcctgc cgcataccga
    14101 ccccaggctt tttccatcgg ttcgtatccc tcaacgagac tgccgaacag atgaatccgg
    14161 acctttccac ggggccttgc catgagatcg aaccatcacc cggacatcaa gatcaggcgg
    14221 gacgagagac cctttttgag cggagttcaa gtccttttgt gcctttcggg gatcttgctg
    14281 gtgctcaatc ttgtcttgtt gtacgggatt ttcttctccc cccaggggat tttcggattt
    14341 cgacagcaat atcagcagat cgttgccatg gaatccagga ttttcaacct aaaaagcgaa
    14401 aaccagaagc tttttcgtaa aattcaaagc ctcaagtccg acccccaggc ccaggagcgt
    14461 ttggttcggg atcacctggg gtgggtgaag cccaatgagg tcatggtgga gttccctccc
    14521 cctcgaaaag atccccgctg atcccggggt ctcttcctcc ccggcgtgcc ccaatcccgg
    14581 gctcgggggc caaaaaaacc ttgacgctcg gaaaaccaat gtgttagcta tcccactttg
    14641 cggaacaccg ctccttgctc ctcccttcat cggggagggg ctgccaaccc acaaggagga
    14701 tcaggaatgc gcaagtacga gacttttttc atcgtcgatc ccgacctgcc cgacgaagtc
    14761 cattccgtca tcgacgacaa actgcaatcc attgtccaat ccaacggcgg catggttttg
    14821 tcctacgttc cctgggggaa aaggaagctg gcctacccca tcaagaagcg gtctcgaggg
    14881 ctttacgtgc tcatggaata tgccggagga tcgaagttgg tggcggaact ggaaagaaac
    14941 ctccgattgg acgaacgaat catcaagttc ctcaccgtga agctcgacga caagtttgat
    15001 ccggcggccc tggaggctgc cgaagctccc gcccgggagg ccgtcgcgga agtagaggag
    15061 gcccccacct cccccccgcc ggaggaaggt gcggaggagg cggaagaaag aaccccggaa
    15121 cccctggaat aagacggcgg gatcccgaga tctcgtagct gcaatggaga ggactcatgg
    15181 cgattcgacc caaaagacgt acctttcacc ggcgcaaggt ctgtcggttt tgcatggatg
    15241 gcgaactcaa gatcgattac aaggattcca aaaccttgcg gtactttgtc actgaaaggg
    15301 gcaagattgt cccgagaagg atttcgggaa actgcgccaa gcaccagagg gaattgaccc
    15361 tggcgatcaa gagagcgcgg caaattgctc tgcttcctta cacgacgatt cacaccattt
    15421 gacgagaatg ccccggttgc accctttggt cgttcccgtt cgtgagcgaa cacaccgatg
    15481 caccaccggg gagcggaact cgtgttcgcc tccaacgggg gcggcacgat caggctttgc
    15541 ctgtccgtga ttctttcacg gtgaagttga atggctattg gaaatggagc atcaccgggc
    15601 cgggtagggc agaggggaaa agaagttctc ctggggattg ggggtacgct ggcgtttttc
    15661 ttttcggcga tcttcgtccc catggtgggg ctctttgcgg gaatctttac tccctttccc
    15721 actttgctct ttttttatct ctggggtccc ccctggggat atgcaatccc cggaggcgcg
    15781 gctctggttg ggaccctcgt cctcgccgtg ctgaacctgg ggcaaaacgc gccatacctc
    15841 ctggagatgc tggttttcgg gctttttctc gggtttgcca tgaggcgccg gtggcccgtg
    15901 gaaagggtgg tggccgcttc cacagccctg gtgaccgcca tcggggcctt tctcttgtgg
    15961 acctcgcttc atggaacgga agccgggttt ctcaaggcgt tggaggagga cttcggaaac
    16021 tccgttactt cgaccctgca gcaatacggg gccttgtcca aagaccatgg ggagatgaac
    16081 gacgccgttc agcgggtgat tcccgtcatg gtgcggctct ttcccgccat agccgtttcc
    16141 tccgccatcc tcatctcctg ggtcaacctc ctggtgaccc ggcgatactg ccggaaccga
    16201 ggcgtgtcgc tgcctccctg gggggaatgg tcccattgga aggccggcga gtccctggtg
    16261 tggggggtga tcgcagcggg tttttccctg ctgctccccg tgaagatgct ccagttggtg
    16321 ggcctcaacg tgctcatggt gatgggaacc atctatctgc tccaggggtt ttccatcctt
    16381 gccttctatt tcgagcgatg gaacctgccc cgggcgctgc gggccttttt gtatgctctg
    16441 ttgtttgtgc aacagtacct ggccctgggg gcggttctgc tgggcctttt tgacgtatgg
    16501 gccgattttc ggcgcctgtc caaaaaaccc gtcgagcccg cctgacccgg cttctgccgg
    16561 tacgtgctcc cgtgcctccc tctgaacggg agggaccggc caaggattgc cggggaagga
    16621 cggcgttggt ttacatcagg acacggcgca tcatgacttg ctggaatgac gagagttgcc
    16681 ggaaggagag gaacatgaaa gtcatcttga ccgaaaacat cgcttccctg ggagaaatcg
    16741 gagacgtggt gaacgtcgcc ccgggatacg cacgcaattt cctgcttccc cagaagatgg
    16801 ccatggaggc cacgggacga aacgtgcgcg aactggatca caagaagcgg atgctggctc
    16861 agaaacggga aaagatccgc caggaaatgc tctccctggc cgagaagctc aaccaggtga
    16921 agatcaccct tcgacgaaag gtatccgaag aagacaagct gtacggctcc gtgaactcct
    16981 ccgacctcac gaacgtgctc caggagatgg gattcgacct gccccgcaag agcatttccc
    17041 tggaacatcc catcaaggag ctgggggagt actccgtgcc ggtgcgggtg gacgctcaga
    17101 ttaccgccac catctccgtc accgtggaga aggaagaata gagtcgatgg acgccgttgc
    17161 tgaagagctc aagaaggtcc ctcctcagca gattgaagcc gaacagtccc tgttgggcgg
    17221 tctcctcatc gacaacgacg ggcttccggc ggcgttcgaa gtgctccggg gcgatgaatt
    17281 ctaccgggac ggtcaccggt tgatttttcg ggccatccag gagcttttcg accggaacga
    17341 gcccgtcgac ctggtgaccc tggcggatca cctgggggaa aaacagcaac tcgagcaagt
    17401 gggagggacc agctacctgg cctccctcat cgaagcggtg ccctcggcgg ccaacgttcc
    17461 cgtctacgca aagatcatca acgaaaaagc cctcctgcgc cgcctcatcc aggcagccgg
    17521 cgaaatcatt tcccactgct acgggggagg aaaaagtgtc gaggagatcc tggacgatgc
    17581 cgagtcggcc attttttccg tcaccgacaa ccggatccgc agcagctatt tcctcatcaa
    17641 ggacatcgtc aagaagagca tcgaggccat cgaacgatac caggaacatc gggaaatggt
    17701 ctcgggggtc ccttccggct tcaaggacct ggacaagctc accgccgggt ttcagccgtc
    17761 ggatctcatc atcatcgcag ggcgccccag catgggaaag acggctttgg ccttgaacat
    17821 tgccaggaac gtcagcgtgc aaaacaagat ccccgtgggg ttcttctccc tggaaatgag
    17881 caaggagcag ctggccatgc ggctgctgtg catggaaggc atggtggatt cccacaagat
    17941 tcgcaccggc tttctcagcc gacaggaatg cgccaagctg ctcacggccg cgggggcctt
    18001 catggacgtg cccctgttta tcgacgatac ccccgccatc acgagcctcg aactgcgggc
    18061 caaagcccgg cgcatgatgg ccgacaaggg cctcggggtg gtcgtcgtgg actatctgca
    18121 gctgatgaga agccgggaag gggcggaaag acgggaacag gagatctccg agatttcccg
    18181 atccctgaaa gccctggcca aggagctcaa tgtaccggtc atcgcccttt cccagctcaa
    18241 ccggaaggtg gaggagcgca acaacaagcg gcccattttg agcgatctgc gcgaatcggg
    18301 agccattgaa caggatgccg acgtcatagc cttcatctac cgggacgagg tctacaatcc
    18361 ggattcggcg gacaaaaaca ttgcggaagt cagcatcggc aagcaacgaa acgggcccat
    18421 cggcaccatc aaactgcatt acatcaactc ctatacccgt tttgaaaacc tggcccacgg
    18481 gatataggat gccccgggat cctgaaaacg ctcaaccggc gtacccgtac ggcacaggac
    18541 ccagtacccg cggggtcgaa tttcgtggta ccggcgcgtg gtacttttga tccgaaaagt
    18601 accacggatg ttgcaaaaaa caacaggggg cgcaaatgaa aaacgaggct acaagccatt
    18661 ctgaggattt acgggtttct catcttcgtt ggtggtggac ggagaggtca agtcatcaat
    18721 gtattacgac aagccattct atgagtacca cattcccggc gtggtactgt tgcgtggtac
    18781 ttttcctttt ttttctgttt ttgatcccca cagcaaccat ggcgggagcg aaggagaatt
    18841 tgctgctgat cgggtgggac ggggtacaat acaatcacct tcaggaaatg ctctcggcag
    18901 gagagctccc aaatctatcc aggtttccgg ttctggtacg aaccgagatc acggatcatg
    18961 caacctcgac caagccgggt tgggttcaga ttcttaccgg gcttgaagca tccgtatcgg
    19021 gaaccagaag taacggaatc taccggccat ttcctcaacg agcctgcctc ttcgatatct
    19081 tgaagaaaga tgctccaggc cgaaacctct ggaccgcatt tgttgcaggg aaggatcatc
    19141 acctagggag catgggacct ggggttagat ggatcgatgg ggatgggaag ccaacatctt
    19201 cgagagacgg ggaaccgtgg tttcacgcca ggaaatcgtt cgatctctgg atgggagaca
    19261 tggaccgcga tgccgatgag gtgggtactc ttgcgatcag tcttcttgat cggtttgcaa
    19321 aacggaatca attcgcagta ttcgtccact tcgcggatcc ggattgcaag ggacatagat
    19381 ttggagagaa ctcacccgaa tattctcagg ccgtttccga ttgcgatgcc tggcttggga
    19441 ggatacttgg gaagctggat gagttgggag tcgccgggaa aaccttggtg gccgtggtga
    19501 cggatcatgg attcgacgaa ggtgaattcc gtcaccaccg gtcttatgat gcatggcttg
    19561 cggtccgtac ccatcggaat ccgaggtggc atgacggaga ccagaaagat gttgcaccca
    19621 cattacttag aatcatggga tcaggaaccg aaggaatgac cgggaaacct ctatggtggt
    19681 aagatctcct ttgttgccta caacctcata accttcggaa ggtggaggag cgcaacaaca
    19741 agcggcccat tttgagtgat ctgcgcgaat cgggagccat cgaacaggac gccgacgtca
    19801 tagccttcct ctaccgggac gaggtctaca atccggattc ggcggacaaa aacattgcgg
    19861 aagtcagcat cggcaagcaa cgaaacgggc ccatcggcac catcaaactg cattacatca
    19921 actcctatac ccgttttgaa aacctggccc acgggatata gggatgcccc gggaccctga
    19981 aaaagccccg cctgcgtgcc gtccggcaca ggacccggta cccgcgggca cctttgcggg
    20041 ttcctgcaac cgtgcatttt cgtccacacc ctcaagcccg tcgtcatgaa tgtctgccac
    20101 tcccccataa ccggaaagaa ccacccatga aaagaaccga tcagtattgg gacgcctccg
    20161 tcgaggccct ttcccgttcg caactcgatc agttgcagct tcggaaactc aaacaggtcc
    20221 tcgaccgagt gcaaagctcg gtgctctacg gcccgagatt gaaagaggcg gggctctcgt
    20281 ccgcaggact ctccgccatc gaggacatcc gccggattcc tttcaccacc aaggcggacc
    20341 ttcggagcca gtatcccttc gggctggtgt gcgtgcccaa ggatcggctg gtgcgcctgc
    20401 acgtgtcttc gggcaccacc ggacaggcca cggcggtcct gtatacccgg ggggacgtgg
    20461 accgttgggc ggacctcctg gcccgctgta tgtacatgac gggggcacgc ccgggtcata
    20521 ccttccagaa ccttaccggc tacggcatgt tcactggcgg cctggggttt cattacggct
    20581 ccgaacgcct ggggctgttg accatccctt ccggggcggg aaacagcaaa cggcagatcc
    20641 agctcatgcg ggatttcggc accaacattc tgcacatcat ccccagttat gccctgcgcc
    20701 tgatggacgt cctgggagag atgggaatgg atcccaagca cgatctcgag ctgaagatcg
    20761 cctacctggg ggcggaaccc tactccgaag aagtccggcg gcgggtggag gacttctacg
    20821 gcatcaaggt ttataatagc tacgggctct ccgagatgaa cggtcccggg gttgccttcg
    20881 agtgtccctt ccagtcgggc atgcatattt gggaggatgc ctacctggtg gaaatcgtcg
    20941 acccggaaac cctggaaccg gtggaagacg gaacgttcgg cgagctggtc ctgaccaccc
    21001 tggaccggga agccatgccc atcctccggt atcgaacccg ggacgtcacc cgcatcctgc
    21061 cgggcgattg tccctgcggg cgtgtccacc gcaggctgga ccggatccgg ggacgctccg
    21121 acgacatgtt catcatcaag ggggtgaaca tctttcccat ccaggtggaa cacgtgctca
    21181 tgaacatccc cgaggtgggc aacaactacg tgatcgtgtt gcgccgggaa aacgatatcg
    21241 atgaaatgct ggttcgggtg gaggtgaccc aggagctatt cgtggaggac atgcgtcatc
    21301 tgcaccgggt tcggaaggag atcgtgcgaa atctgaagaa cgagctcctg gttacgcctg
    21361 ccgtcgagct ggtggaaccc aacagtctgc ccaagagcga agggaaggcc gtccggttgc
    21421 tggatcaacg aacgccccgg aaaggaggtt gagcccattc gaatttttgg cgacacttcc
    21481 ggtctcaagg cgacccaccg gcagagaatt cagaagctct accgacggaa gatccccccc
    21541 cagcaggtga tttctccgga tcttgcccgg gatcttgccc ggatctctta cgagtgcggc
    21601 cgccaaatcg gtctcctcgt tgcccgaagc ggcaccgtgg aaatggtgct ggccggaggg
    21661 cccgggtccc tctacattcc cgatctgccc aaaagccgcg gcgggcgcag ccacctgcgc
    21721 gggcttcgcc tcgtccacac ccacctgggg gaagaacccc tctcccagga cgatctcatg
    21781 gacctggtct ttctccggct tgactgcgtg gcggccgtgt gcgtggaccg gcacggaggc
    21841 gccacccggc tcgaggtggc tcacatcctt cccagcagcc gagaccggga gcccgcatgg
    21901 cggatcctgc cgcccctgcc ttgctacgat ccggacctgg atatccgaca gatggttcag
    21961 tccctggaag aagagctgga gagaaaccgc ctggccgtcg aggcgtcccg ggccgaagac
    22021 cgggccattc tggtgagcgt gaccagcgcc gataaggaga cggccctgga ttccatggcg
    22081 gaactgcagg aactggcgcg cacgggagga atcgaggcag ccgacaccat catccagcgg
    22141 cagagggaag tgaaccccaa gtacctcatc gggaaaggaa agctcagtga gatcgtgttg
    22201 agggccctcc agtgcggggt ggatcttctc attttcgacc aggagctcaa tccttcccag
    22261 gtgaaggcca tcacggacac caccgagctc cgagtcatcg accgcaccca gctcatcctg
    22321 gatattttcg cccggagggc ccacagccgg gaaggcaaga tccaggtgga gatggcccag
    22381 ctcaaatacc ttctgcctcg cctgggcatg aaggacgacg ccctctcccg gctcaccggg
    22441 ggcatcgggg cccggggccc cggggagacc accctcgaga tcaatcggcg gcggaccaag
    22501 gatcgcattg tgcacctgga aacggaactg gccgccgttc gcaagaaccg ggaacagcgg
    22561 cgggcccggc gaacccgaaa ggacgttccc gtcatctcca ttgtgggcta caccaatgcg
    22621 ggcaagtcca ccctcctcaa caccctcacc cacagcgggg tgtttgtgga aaacaagctg
    22681 ttcgccaccc tggatcccac cagccgtcgc ttgcggtttc cccgggacat cgaggtcatc
    22741 attacggata ccgtgggatt cattcgagac ctgcccaagg acctgatgga agcctttgga
    22801 gctaccctcg aggagttgag cgatgcggat cttcttctcc acgtggtgga tctgagcaac
    22861 ccccagttcg aaaaccagat ccgcgccgtg gatcggatcc tcgcggacct gaacctccgg
    22921 caaaaaccga ccctcctggt cttcaacaag atggaccgag cggacccggc cttggtggag
    22981 cgggtgttgc ggcgccacgg cggggtggcc gtctcggccc tgtccaccaa gaccctcgaa
    23041 cccctgatcc tggccatgga agagcaaatc acggcccagc tcgaggaggg attgtttccc
    23101 cgggccttgg gggaaacgga agaggggggc tcggacccat ggacggcaag gtccccattg
    23161 ccttgaacgg gacggagccg gggaggaggg acgagcccgt ttgtgcctgc gacggcgccg
    23221 cctgccgggg cgacgcatgg gcctcgggga tcgaggaagg gggcaaagga gaactcagaa
    23281 acccgggtgt tcggcgagga tctgcctgga tcgttcgatg tcctttcgga tttgaacggc
    23341 cagctcctcg gggcccgaaa agcgtcgctc ctctcgaagg cgatccacga attttaccgt
    23401 gatgggcttg tcgtaaatgg tatcgttgaa atcgaagatg tggacttcga gggagagttc
    23461 ccggtcgttg aaggtgggat tgtagcccag gttgcccgcg cccccgtaaa gcttcccgtc
    23521 gaccaccacc tgcacggcat agacgccggg tttgggagtt acctgccagg cttcggcctt
    23581 gatgtttgcc gtgggaaacc ccaggaggcg cgcgccccgt tggcggccgt gtatgacggt
    23641 cccggagatt tcgtagctcc tacccaggag cttgttggcg gtacgcaggt ttccttcgct
    23701 cacgaggtgg cgaatgacgg tgctgctcac catcgtcccc tcgagctcga tcccttccac
    23761 cgcttccacc ccaaagccgt attggtctcc caactccctg agaaaacgaa tgtttccctc
    23821 tcgaccgtag ccgaaacgat aatcgtagcc caccaccagg gccttgaccc cgatccggtc
    23881 caccaggata ttcttgacga agtcccgggc ggagatgagg gcaaattccc ggctgaaggg
    23941 gatcacgatg gccacgtgga tggacgagtc ggccatgagc tcgatttttc tctggtggga
    24001 ggtgatgaaa gagagctctt cattggggtt gagtacccgc aggggatggg gatagaaagt
    24061 gagaaccacg gattcgccgt tgatctgggc ggcccatgct ttgacccggt tgaaaatggc
    24121 ctggtgtccg cgatgtacgc cgtcgaaatt tccgatggtg agaacagggt tcttgagaga
    24181 cctggggatg ggatcgattc cctcaaacac ttccatgatg agcgtttgca cctccaaaca
    24241 gaaaaaaaat agaccttgac agcacaagaa aattagcata cgaaacctcc agttccaatg
    24301 caacgctttt ttgcgtgccg aagtggcgga actggtagac gcgctaggtt cagggtctag
    24361 tgggcgtacg cctgtcgggg ttcgagtccc cgcttcggca ccacgacaat ccaaggggtt
    24421 aggtcaagcg gcctgacccc tttttctttg tttttagcca caatgggtga cacagaaaaa
    24481 gagatcggcc catccggcta acgagtacct ttgggcatga agagggtaca atctgagctt
    24541 aaaatcttca tggtccctga agccgtaagc ctgtctcttg agggtcttga tttgattgtt
    24601 gatgccttcg actacggcgc t
//
LOCUS       DAOOVC010000050        23757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1453_length_23757_cov_7.299089, whole genome shotgun sequence.
ACCESSION   DAOOVC010000050 DAOOVC010000000
VERSION     DAOOVC010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1453_length_23757_cov_7.299089"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1634
                     /locus_tag="PLM79_08490"
     CDS             132..1634
                     /locus_tag="PLM79_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV86386.1"
                     /translation="MREKDIKREVLKQLKKEFPKWRRLPRKEKQRIAGEVLESVVAKN
                     TGNEPTADIPIHELTNTPVPMPGIIKLAEMEQFVEQTTRSLLSFPVKRWPSRYEDPEL
                     EAIDRLVDDRVVNRLLAPVGYTPSMRDLYPSHYLRAELLKSLRYAEISYRKYCRDVIN
                     RMDSKRQRAFIHLPLHRAITIDHTQLSRFRNSLTVVQLVNLMVYVIHLLVKSGRITHP
                     FRVCGIDSTDVAVLSCPRPLATIEVGNKKVRIYAELEADCGKRRKKRDKSEYFVGYRI
                     HTLVAIDPNTGLNHPLFSLAAPANHHDNLFLPQLIGFGHAMGLSMDIVTVDEGYIDTE
                     QNEQIRKDYGVTIITPAKEKALIPDHVDPNSGAVYLNGHCEVPMSYGGRTETGHEFHC
                     GADECFHAPTCSKCREIPLDAGRFGQIQDQVYGVEQARDIRKNMERCFNLLKHREGLD
                     PLRVKSQHSTLVAATLAQMATVVLEIVGTRKTETKKTLPKQLKMFCCQAA"
     gene            complement(1928..2167)
                     /locus_tag="PLM79_08495"
     CDS             complement(1928..2167)
                     /locus_tag="PLM79_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86387.1"
                     /translation="MSLYIKTEDYRKHGISKYSDLALIRTVVQKELNIDRVFVSFVNK
                     HEYIRVDFLRPRPPRRPRGRQHYRSEKASQETKEA"
     gene            3078..5273
                     /locus_tag="PLM79_08500"
     CDS             3078..5273
                     /locus_tag="PLM79_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOV86388.1"
                     /translation="MTQKLQGRVERITYSNEENGYCVLKVKIPGERNPITVVGNFVSV
                     TPGEVLRMEGNWDHHSRFGTQFKVDRYETTAPDSVEGVRKYLGSGLIKGIGPVMATRI
                     VGLFGEKTLEIIDRESERLLEVEGIGSYRLEQIRKAWEDQKDIRELMVFLRGHGVSAG
                     YAVRIFKHYQKGSLQVVKDNPYRLAMEVAGIGFLSADKIARNLGFAADSPLRAEGGIF
                     YALQQAMEEGHECVPRRRLLEKCVELLEIPAAVLESALDQLEADRHIVVQSLPGDVAQ
                     TFGDQRAVYLKGLHTAETQAAKRLTFLKAFRSLQRKGNPDEALEWLRKELPFQLAPLQ
                     EEAVRRALTDKVVVITGGPGTGKTTLVRAILAVYKRMKASVCLAAPTGRAAKRLSEAT
                     RHGAGTIHRILEFSPQGGGFQRNEQKPLPADLVLVDETSMMDILLLHHFLKAVSPHAT
                     LVLVGDADQLPSVGPGNVLQEIIRSGQFPVVRLTEIFRQARESRIIVNAHLVQQGKFP
                     VLRTESRGLQDFYFIEKEDPEEVLEVILRLCSQRIPVRFNLDPVDQVQVLSPMRRGMI
                     GTQRLNIALQEVLNPQTASLERGGQVYRVQDKVMQIRNNYDKEVFNGDLGRIRKIDRE
                     NQEVHVEVDGRTVIYDFSEMDELVLAYAVTVHKAQGSEYPAVVLPLLTHHYMMLQRNL
                     LYTAITRARKLVVLVGSKKALAIAIRNDKMQHRFSLLEERLRGSLGLEG"
     gene            5285..5680
                     /gene="tsaA"
                     /locus_tag="PLM79_08505"
     CDS             5285..5680
                     /gene="tsaA"
                     /locus_tag="PLM79_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HOV86389.1"
                     /translation="MGEPIVLEPIGIIRSPFHSTDEAPHLGSEAGIEGELVVDPRYRD
                     AMFGLEAGRKVLILYWLHLADRGRLQVHPRGDRTRPLRGVFSTRSPHRPNPIGADLVE
                     VLEIRGTTIRVKGLDALDGTLLLDIKKEV"
     gene            6045..7037
                     /gene="moaA"
                     /locus_tag="PLM79_08510"
     CDS             6045..7037
                     /gene="moaA"
                     /locus_tag="PLM79_08510"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOV86390.1"
                     /translation="MIPPPQDPYRRRIDYLRLSVTDRCNLRCTYCMPREGVQKLPHED
                     ILRYEEILRLVRIVTAMGISKIRITGGEPLVRKDLLYLCESIARFSGLKSLSITTNGV
                     LLPKFAKDLFGAGIKRINVSLDTLRPEKYAAITQMDCFHQVWDGILKAHEIGFRPIKL
                     NAVVMRGINDDEIEDLGRLTFRYPFHVRFIEFMPFQAEAGDQRFLSADDILSRLSRVA
                     PLVAAPKDGDTNGPASHFRFRDALGKIGIISPVSHHFCPTCNRLRVTADGGLRTCLFS
                     TSEVDLRTPLRGGASEERIMEIIRRAIVEKPEKHSLESAVFRKCISRPMFSIGG"
     gene            complement(7328..8302)
                     /locus_tag="PLM79_08515"
     CDS             complement(7328..8302)
                     /locus_tag="PLM79_08515"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOV86391.1"
                     /translation="MALKRFIGIDLGGTHLRSALVDPGGTIHHQRKIVMGPLLDPQVA
                     SRRLVEECRLLLEDARKEGARVAAVGMGVAGKIDGERGTVIFSPNLPTMRNYPLGTEL
                     QEALGIPVFMENDANVFGMGEAWVGAARGISNWVGLTLGTGVGGCLFFDHRLWQGDRL
                     GFSGEIGHLIVDPHGPPCACGLQGCLEAHSSGSALVRGFETAMAEGGSVSQGARKDWE
                     EGKLTPERIYWYARQGEPAAQSLFARMGWALGLALAGLFTVLGIRHAVLGGGVSASWD
                     QFHDPLLKSLSRNSSMLDPADAVILRSELGDRAALLGAAKLAWEGCGP"
     gene            complement(8398..9024)
                     /locus_tag="PLM79_08520"
     CDS             complement(8398..9024)
                     /locus_tag="PLM79_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86392.1"
                     /translation="MAELIDMTRRLPGRDNLITATPLEFRRGEWHGGFTLMCTRRESA
                     RFEAHLEEALKTTGHRHVAIVPTHFTLDGGYHYTVLGLFRNRKDEGRMRRVYRLAGLM
                     ECVTNAPSPILRTDLLRRFYRSILEEREELNVVWRGNIRHFLFPLHPEFYNPNLFLHA
                     VTNAESLKDLFEAVQAQTDTQFDLLTRNYVFYVPENFLIRESHPPQGG"
     gene            complement(9041..9712)
                     /locus_tag="PLM79_08525"
     CDS             complement(9041..9712)
                     /locus_tag="PLM79_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOV86393.1"
                     /translation="MYVGWNMKTNLVTVTPETSIFKAREIMDAHKISHLPVTDGKARL
                     LGIVTDRDLKEAWASPATTLSVHELTYVLQKLTIANIMTRNVVTATPDMAIERAALLI
                     HDRKIGALPVVKQDRLVGIITSTDLMEVLLTALGISEDSKRVSLLVRHRSEVLAQVGR
                     QMEEAKINIRSILTVPLKGHEDVWQLILRVDLADFDRTVKILGDGGHKVLTEYAEDLT
                     PYFPQ"
     gene            9822..10229
                     /locus_tag="PLM79_08530"
     CDS             9822..10229
                     /locus_tag="PLM79_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86394.1"
                     /translation="MSSKKPMDDRKLLEEFSNAIELWEDSPYYLPSEELFDIERFLTD
                     HQAHRLKTDLEHIYGTPLNQKFWSSFLAYHRLKKVRDEIKTQEMLQEERRRRYLEAVE
                     EEKKTWQKWLKDWFHYQQRQKLRILPGGKKNDP"
     gene            10979..13849
                     /locus_tag="PLM79_08535"
     CDS             10979..13849
                     /locus_tag="PLM79_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV86395.1"
                     /translation="MNAGSIVKFRNREWVLLPSDSPEVIRLRPLTGVTDDVVKVHRRL
                     SNLLGYSFPEERVSPATFPLPHGDAIADAASTHLLWQATRLTLREAAAPFRSLGRISI
                     RPRTYQFVPLLMGLRIDPVRLLIADDVGVGKTIEALLIAREMLDRGEIKRFCVLCPPY
                     LCDQWQKELAEKFNLDAVLIRSGTVAQLDRAKTRSESIYRYYPTQVISIDFVKSDRNR
                     HAFLLDCPELVIVDEAHGVAVAGEGLFSQHQRHELVQEIAAKPDRHLLLLTATPHSGI
                     DSAFRSLLELLDPQFSAWIPSALNEAQRSRLAGHFVQRTRRDIEKNWEGQHCFPQRDP
                     SDETYRLSSEYSDLFNKTYEFCREMVRSGSRLAKPQQRVRYWGALALLRCVMSSPAAA
                     LAALAGRYHGLPGTEDEIDFRTFVFESSDDRTDDNQPTPPVQSAEETLNYRDRRRLRG
                     LGRHAAALQHKPGDTKLTTCISLVRRLLNEGYRPILWCRYIATADYLAEGLTRSLANE
                     FDGLRVVSITGNLTHEERRAKVEELLHERVRVLVATDCLSEGINLQQGFNATLHYDLP
                     WNPNRLEQREGRVDRYGQTAPTVKTIRYFSPDSPVDGVVLEVLLDKAREIHRVLGTHV
                     PVPEESETVTEAVLNALFLRGRPAPNEPSKDGPIQLTMWPKVASFHRKWDLDAEREKV
                     NRTRFAQRTLKPEEVRRELEATDAVLGDPEAVRLFVLNAAQRLGLSITEDRRIPDAYR
                     VATGPEIRAILPDAVSFALPDSRVAQWLISFVSPTPEGAEYLGRNHRFVTALAQFLLE
                     EALVKGADARAARSGVIRTTAVPRLTTLYLIRVRYLLEQPDRLPALAEEVQVLGRRME
                     KDGKEMWLSPDEALLLLSAAKSEAIVSMVEKRELVSMALDEWERIMPALRRRLDERAA
                     ELLDSHRRVRRAVAMKVRGLAFKPQLPPDLLGMLILQPMVKL"
     gene            14110..15414
                     /locus_tag="PLM79_08540"
     CDS             14110..15414
                     /locus_tag="PLM79_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86396.1"
                     /translation="MLTRFRLENFRSYHGATLDLSPLTLMVGANASGKSNALEALQML
                     SWLSRGRRLEDVFRAVEQMELLIRGRVTDLTYEGAQTFRLGCSLQQAGPWRDLDVEIA
                     VDKEGMRIRQESITSPTERSPLYKVEQPATGFSHDILVSYNNFARGGIKPQISCTDQQ
                     GVFTQLLTPARFANHHTRSQKEIPSTAKVFIQALERILFLDPVPRRMREPSYVFDKAL
                     KGDGSNISSTLHDLCVGQQAKEKVLDFIRDLPEQDIADLQFLEGPRQDVMVQLEETFG
                     GTGKLREAALLSDGTLRVLAVAAALLSAPAGSMVVVEEIDNGVHPSRASRLLANIESV
                     AREKNLAVLVTTHNPALLDALPDGAVPNVVFCYRDPQKGDSRLVRLSEIPDFPDLVAQ
                     GPLGRLLTQGIIDRFVKTARSPEEKVRAGLEWIHNLRKTAAS"
     gene            15411..15863
                     /locus_tag="PLM79_08545"
     CDS             15411..15863
                     /locus_tag="PLM79_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86397.1"
                     /translation="MSICLIDTSVFCNVVDVPGRNQQRGEVLNSLEMFIRQKASLLLP
                     VVVLLETGNHIGHVSDGGLRRLAAERFVEQVAAALDGRAPWKPTPFVEADQLRIWLKE
                     FPDHAGRGAGLGDLSIIKEWERQRRLNPNRRVFIWAFDKHLSSYDTQC"
     gene            15892..20124
                     /locus_tag="PLM79_08550"
     CDS             15892..20124
                     /locus_tag="PLM79_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86398.1"
                     /translation="MSFPSVRIEGGLLGPDILDLLVSGDLAGQKPVDFGLEARRNLTD
                     EIAATFADARALWRIFQHRLERLAPDDPATSVTRDAWMIPFLGLLGYELQYNPRAYDV
                     EGLTFAISHRAVSSAAGAQTGAAEVVDGPPVHIVGARQELGKLASSGRPRLAPHSLVQ
                     EYLNRTEFVWGIVTNGLTLRLLRDSTFVRRQAYVEIDLTGMFEEQRFGDFAAFYRLLH
                     RTRLPHGPADAATCFLEQYHTRSLVEGSRIREHLRDGVQRCIEILAEGFLSHPANEDL
                     RREIEEGRLTPEDLYRQLLRLIYRFLFLLVAEDRGLISQNALYCEYYGIGRLRNLFDR
                     RGAFSDHEDLWYSLRVLWHVLSDERLSSILDVAPLNGELFRSQTLDDCILTNRNLLEA
                     FRHLAWYRENRTAQPRRVNYSALDVEELGSVYESLLELHPHLDASIVPPRFELIVSGR
                     ERRSTGSHYTPPELVAPLIENALGPVIQEKLGEAKRIANGEWRTEQERNHALAVVWRE
                     RVEDGEWRVVNGEWRSKDGRVVSRSRCVEAGHDIGERGVQVDDLVAEGRALWADLADS
                     ACGKLHSGEHRRRLGQEIPCGIHPVPEEGKRQPHRAGDTSPVGRGDSALPDGTDRPAP
                     ERTGDPRQADPEPGEESRSPHQNLTSIDQFTQPATHHSPFATHPSLLTHWKSTPFATR
                     YSLLAQKALLTLRVCDPACGSGHFLLAAARRIGKELARVRTGEQEPAPEGVREAVREV
                     IHNCIYGVDKNPLAVDLCRVALWIEGHTTGKPLTFLDQRICCGDSLVGVLELKDLDGG
                     IPDAAFKPVTGDDRTAAREAKRRNAAARGATLFQFPSSRRLQVLAQQLRSLGHLPEDT
                     MEQVRDKAESFSRLVQSDDYQRLKLACDVWSAAFFQPFPGPSAMPVTTADVQMALMKG
                     RLSDPRMTAFVQETAAKQRFFHWPLAFPEVFGGGGFDAIIGNPPFLGGLRVSGEYGLK
                     YRQWLSIAFEPFTGTADLCAAFFRRSFHTIRPEARFGLIATNTIGQGDTRESGLAPIV
                     SKAGSIVFARRFVKWPGAANVEVNLVAVRKGPSESEFRLDDQLVPSISSRLDDEPEAE
                     PHALRQNQGKAFQGDIVRGIGFVLEPGEASQILHRDPQNGDCLLPYLNGEDLNTHPQQ
                     EPSRYVICFHDWPLKRAEQYPDLLRIVEERVRPERERLRNRDDRHDREYWWLFARYRS
                     DMRPAIGPLSRVMARSRVSELHAVSLVPKGWICNEQTVVFAFEDFFHFALIQSNIHEA
                     WLRRNASTMRTDIRYTPTDCFETFAFPQNPSEASRSFAEQLGASYHAHRRQIMLDRQI
                     GLTKTYNLFHNPQATDADILRLRELHAEMDMAVLACYGWDNLDLEHGFHENERGQTRY
                     TISSSARRDLLRRLLALNLDLFDQETRERNPE"
     gene            20152..20718
                     /locus_tag="PLM79_08555"
     CDS             20152..20718
                     /locus_tag="PLM79_08555"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOV86399.1"
                     /translation="MVEPAKKRATYEDLSNLPENMVGEIIDGELVVTPRPARRHIDAA
                     ASLGGELIPTYRFGRGGPGGWIIYHEPEIHLGEDVLVPDLAGWRKERLTTAPEEHRFV
                     VAPDWICEILSPSTIRVDRVKKMRIYAANGVPHVWIIDPAAKTLDVFRLESGKWLLLD
                     TFVEDDRVRAEPFQEIEIELGNFWVAES"
     gene            20834..>23757
                     /locus_tag="PLM79_08560"
     CDS             20834..>23757
                     /locus_tag="PLM79_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV86400.1"
                     /translation="MSIFDLHANVIADYRNFVRAFITVADDRVRGFLDRAMDEEQRLW
                     PESLLQLSPSYDRVATVAELAAQGVLHEETARIFCTPDGRPFHLYRHQVEALEKAREG
                     ESYVVTSGTGSGKSLTYFLPIIDALMRRPATGERVAALVVYPMNALVNSQVQALEKLR
                     ESYQRRTGLPFPVTFAKYTGETAEAVRGDLRAHPPQIMLTNYVMGELLLVRPEDQRFL
                     DRAGGGLRFLVFDELHTYRGRQGADVAMLIRRIKERCAAPELVCVGTSATMVANRDAT
                     PQERRASVADFASRLFGISLTSEQVIEETLVPVTEGGSPSHRELVEGLSAPLPEDLPE
                     LRRHPLARWAEAEFGVEPEAEGRLKRRVPRPLSEAARRLAEETGADFEVCRERLREIL
                     IRGSEIPSEEGTRAFAFKLHQFVSQGRAVYATIESRDRRELSLDGQLAASQGRVFFPI
                     KFCRQCGQEYYHVLGGGPGEPFRPHPIGTESVDVNARVGYLMPASEVDDWNPGRIPVE
                     WLDRRGRLKPTWKERVPIEVWVGPDGTRYDRAHQGAVRMWLQSQLFSLCLSCGEFYTA
                     REREFTKLAGASSEARSSATTVLASSLLRHAEEEGGARDKLLTFTDNRQDASLQAGHF
                     NDFVHVSLLRSALYTALVKNQQLAFDSVAEATVRETGLMIRDIARNAELDPDSPAARD
                     VWQAFTELTEYRLYEDLRRGWRVVQPNLEHVGLLRVGYRGLEDLCSEDRRRQFHPAAA
                     GASSEEWFFLTRAVLDLFRRKLAIAARCLMETQQQQIRRRSEQHLNEFWGLDPAGGDL
                     RAANRFVRLGRSSRTVEGFSLNIRSTLGRFMSERLGLDATDYDLFLDGFLALLVSLGF
                     LVRLEPVDDHQFYQLDASCLLWQVGDGSPPPPDPIYSCRASGTGYTDAAPPVNAFFRD
                     FYRGEAARLASLEAREHTAQVVRSGERERRERRFRWEEGDVQRRELGRRLPYLVCSPT
                     ME"
BASE COUNT         4894 a         7005 c         7053 g         4805 t
ORIGIN      
        1 aaaaaatcca cccgggaaag aggggttgag ctcaccccgc taattcaaca tcctgttagg
       61 catggaaagc tggtatattc aaggggtatt tccgaccaag aaaaaccact cgaaacatac
      121 aggagctttc catgcgagag aaggatatca aacgcgaagt tctgaaacaa ctgaagaagg
      181 aattcccaaa atggcgccgg ctgcctcgaa aggagaaaca gcggattgcc ggggaagtct
      241 tggagagcgt agtcgccaag aataccggga acgagccgac agcggatatt cccattcatg
      301 aactgacgaa cacaccggtg ccgatgcccg gaatcatcaa gttggcggag atggagcagt
      361 tcgtcgagca gacgacccgc agtctcttga gctttcccgt gaaacggtgg ccgagccgct
      421 atgaagaccc ggagctcgag gcgattgata ggttggtgga tgaccgggta gtgaaccgtt
      481 tgttggctcc tgtcgggtat acgccgagca tgagggacct gtatcccagc cactatttac
      541 gtgctgaatt gctcaagtca ttgcgctacg cggagatcag ttatcgtaag tattgcaggg
      601 atgtcatcaa ccggatggac agtaaaaggc agcgagcctt catccacctg ccgctccaca
      661 gggcaataac gatagaccac actcaattga gccggtttcg aaacagtttg acggtcgttc
      721 aactggtgaa tctgatggtg tatgtgatcc atctgttggt caagagcggc aggataacgc
      781 atccattccg ggtttgcggt attgattcca ctgacgtggc cgttctatcg tgccccagac
      841 ccctggcgac gatcgaggtg ggcaacaaaa aggtccggat ctatgcggaa ctggaggcgg
      901 actgcggcaa gcggcgcaaa aaacgggata aatcggagta cttcgttggc taccgcatcc
      961 acaccctggt cgcgatcgac cccaacacgg gactcaatca tccgttgttc tcactggctg
     1021 cgccggccaa ccatcatgac aatctgttcc tgccccagct gattggtttt ggtcatgcca
     1081 tggggctttc catggacatc gttactgtcg atgaaggcta catcgacacg gagcaaaacg
     1141 agcagatccg gaaagactat ggggtgacca tcatcacacc ggccaaggag aaggccctta
     1201 ttccagatca cgttgacccg aacagtggcg ccgtctattt gaatggccac tgcgaagtcc
     1261 ccatgagcta tgggggcagg acggaaacgg gccatgagtt tcactgcggg gccgatgagt
     1321 gcttccatgc tcccacttgt tcgaagtgcc gggagattcc actggatgct gggcgcttcg
     1381 gtcaaatcca ggatcaggtg tatggtgttg agcaggccag ggacattcgt aaaaacatgg
     1441 aacgttgctt caatctgctc aaacaccgag agggcttgga tcctctgcgc gtgaaatcgc
     1501 agcactcgac gttggttgcg gcaaccctgg cgcagatggc caccgtggtt ctggaaatcg
     1561 tcggaacccg caaaacggaa accaagaaga cattacccaa acagctgaaa atgttctgct
     1621 gtcaagcagc gtaaccgatc cggcggggtc gcgggcaatt ctctctacct tattgaacaa
     1681 tggagggttc aaaacagggt gagcacaggc atgtccacat gaggacatga cgccgcatag
     1741 gccaccggcc ataattcctt cctctgctca aaaggtggga ggagtcccca tcgacctcga
     1801 atttttctcc caaaaagcga cacgtgactc cgttaaccgg tcgaaataga cggcttttca
     1861 acaggcttta ctagacaatg gggtacactt cagattcaga tgtatttgtt gctttttgaa
     1921 caccctgtta ggcttctttg gtctcttgac ttgctttttc gcttcggtaa tgttggcggc
     1981 ccctggggcg gcgcggaggt cgaggtctca aaaaatccac tcggatgtat tcgtgtttgt
     2041 tcacaaagga tacgaaaacc cgatcaatat tgagttcctt ctggacgacc gttcggatca
     2101 atgccagatc cgagtatttg ctgattccgt gtttgcggta atcttccgtc ttgatgtaaa
     2161 gcgacatcgg ttggcctctc tactccccta tgtggtttaa ccgcttccgg ccttcctcgg
     2221 ttctgctgcc gaaaactccc gaaggccgtt cctttccgga aggcacctcg tacgccccag
     2281 ccctccgaaa ggctttgcgt ctatatatca tgctcggtca aattgtcaaa gtgatattca
     2341 ttcatgattc cagtaggttc tccgggacaa tggtgttaca tttcattccg ggaacggtct
     2401 gccgcagaac ctgcacccac acacggcccg cggtcgcctc atcgcgagga gcggccaggg
     2461 gcgtgagatt ttctcccggc ggggcttacc ggggaggcgc atccctcctg ggggcgggaa
     2521 aggaagcgag agcccttttg ctttcgagaa gaacgctcac ggggttgtgc cgcggggggc
     2581 ggccaagctt tttgactgaa cgttcccccc tccccgtagg taccaggcgg ggagcggggt
     2641 gccggggaat tctccaaata cccggattcc cgcccggtcg ggagtcacga acgtcgttca
     2701 agtcagggtc attgaggggc ggcggccggt cctggaaggt gagcttggat ttcgtctcag
     2761 acaacgaggt cccccccggg actctgggcg gttgaccaaa cgggtccccc cgggccgagg
     2821 ccccaaagcg gttgagttgg tgaaacggcc ctgtaggaat tcccccccct cgacggggca
     2881 ctgggacggt agggggagtg tgagcgcacc ccaagcaccc ccttgaaggg gggaatcaaa
     2941 ccgcttgatt caacggcatt gggccaaggt ccgggtcccg ggagcctccc gacccccgaa
     3001 ttccgatccc ggcaggaagg gggttcgagg ctttggtcga ccatcctgcc cccacggggc
     3061 ttttcccgag aagcgccatg acccagaaac tgcagggacg ggtggagcga atcacctaca
     3121 gtaacgaaga aaacgggtat tgtgtcctca aggtaaagat ccccggcgaa agaaatccca
     3181 ttaccgtggt gggcaacttc gtttcggtca cccccggtga ggtgctgcgc atggagggga
     3241 actgggacca ccacagtcgc ttcggcaccc agttcaaggt ggatcgctac gagacgacgg
     3301 ccccggattc ggtggagggg gtccgaaaat acctgggttc cggcctgatc aagggaatcg
     3361 gccctgtcat ggccacccgc atcgtgggac tttttggaga gaaaaccctt gagatcatcg
     3421 accgggaatc cgagcggctc ctcgaagtgg aagggatcgg atcctaccgc ctggaacaga
     3481 tccggaaggc atgggaagac cagaaggaca tccgggagct gatggtgttc ttgcggggac
     3541 acggagtcag cgccgggtac gcggtgcgca tcttcaaaca ttaccagaag gggtccctgc
     3601 aggtggtgaa ggacaacccg taccggcttg ccatggaagt ggcgggcatc gggttccttt
     3661 cggcagacaa gatcgcaaga aacctgggat ttgccgcgga ttcgcccctc cgggccgagg
     3721 gcgggatctt ctacgctctc cagcaggcca tggaggaagg ccatgaatgc gttccccgac
     3781 gcaggctcct ggaaaaatgc gtggaacttc tcgagatccc ggccgccgtc ctcgagtcgg
     3841 ccctggacca gctggaagct gatcgccaca tcgtggtcca gtcgttgccg ggggatgtgg
     3901 cccagacctt tggggaccaa cgggcggtgt atctcaaggg ccttcatacg gcggaaaccc
     3961 aggcggccaa acggcttacc ttccttaagg cctttcgatc ccttcagcgc aaagggaatc
     4021 ccgacgaagc cctcgagtgg cttcgcaaag agcttccttt ccagctggcg cccctccaag
     4081 aagaggccgt gcgccgggct ctgaccgaca aggtggtggt gatcaccggg ggaccgggga
     4141 cgggcaaaac caccctggtt cgagccatcc tggccgtgta caagcgcatg aaggcaagcg
     4201 tctgtctggc ggcccccacg ggccgagccg ccaagcgtct cagcgaagcc acccgtcacg
     4261 gggcgggaac cattcaccgg atcctggaat tcagccccca gggaggcggt ttccaacgaa
     4321 acgaacagaa gccccttccc gccgatctcg tcctggtgga cgaaacgtcg atgatggaca
     4381 tccttcttct tcaccatttt ctcaaggccg tctctcccca tgccaccctg gtcctggtag
     4441 gggacgcgga tcagctccct tcggtgggcc ccggcaacgt cctccaggag atcatccgct
     4501 cggggcagtt tcccgtggtg cggctgacgg aaatcttccg acaggcccgg gagagccgga
     4561 tcatcgtgaa cgctcacctc gtccagcagg gcaagttccc cgtccttcgg accgaatccc
     4621 gggggctgca ggatttctac ttcatagaaa aggaggatcc cgaggaggtt ctcgaggtca
     4681 ttcttcgcct ttgctctcag cggatccccg ttcggttcaa cctggacccg gtggaccagg
     4741 tgcaggttct gagccccatg cgtcggggaa tgatcgggac ccagcggcta aacatcgccc
     4801 tccaggaggt cctcaatccc cagaccgctt ccctggaacg aggaggacag gtctatcgtg
     4861 tccaggacaa ggtcatgcag attcgaaaca actacgacaa ggaagtcttc aacggagacc
     4921 tgggccgcat tcgaaagatc gaccgggaaa accaggaggt tcacgtggag gtggacggcc
     4981 gaacggtgat ctacgacttc tccgaaatgg acgaactggt cctggcctat gccgtaacgg
     5041 tgcacaaggc ccaggggagc gaataccccg cggtggtcct gccgcttctc acccaccact
     5101 acatgatgct gcagcgcaac cttctctaca cggccatcac ccgggccagg aagctggtag
     5161 tgctggtggg aagcaagaag gccctggcca tcgccatccg caacgacaag atgcagcatc
     5221 ggttcagcct cctcgaggaa cgcctccggg gttccctggg gctggaaggg tgaggaaggg
     5281 aaaaatggga gaaccgattg tccttgaacc catcgggatc attcgcagcc cctttcactc
     5341 gacggacgag gccccgcacc tgggcagcga ggcgggaatc gaaggggaac tggtggtgga
     5401 tccacggtac cgggatgcca tgttcggcct cgaagcgggc cggaaggtgc tcatcctcta
     5461 ttggcttcac ctggcggacc ggggacggct ccaggtgcat ccccgggggg accggacccg
     5521 gccccttcgg ggagtctttt ccacccggag ccctcatcgg cccaatccca tcggggccga
     5581 cctggtggaa gtcctggaga tccggggaac caccatccgg gtgaaggggc tggatgccct
     5641 ggatgggacg ctgctgctgg atatcaagaa ggaagtgtga cctggtggac ccgggatcga
     5701 tccatgggat cgggttgccc gcttcctgca agtggctttc ttccaggttt gccgctataa
     5761 tctttccaat cgggttgcgc cgcgggcggc gggttttcga cgaggcccgc ggcggaacgg
     5821 cacgagataa gggaaaaaca ggacaggaat ccgggcacgg ggttcgccgg ggaagaaaga
     5881 ccggtttggt cctccgggtc cgcaacgcgg cgagtccttc gaaccccgga gggcggttca
     5941 aggatttgtc attggacgtt cccagggacc ctgcggcggc tcaatttcag gggggtccat
     6001 ccccgaccat tcatgttgaa accctccagc ggaaggaacg gctcatgatt ccacctcccc
     6061 aagaccccta ccggcgccga atcgattacc tgaggctctc cgtcaccgat cggtgcaatc
     6121 tgcgctgcac ctactgcatg ccccgggaag gggtgcaaaa gctcccccat gaggacatcc
     6181 ttcgctacga ggaaatcctg cgactggttc gaatcgttac cgccatgggg atcagcaaga
     6241 tccgcattac gggcggggag cccctggttc gaaaggacct gctctacctg tgtgaaagca
     6301 ttgcccggtt ttccggcctc aagagcctga gcatcaccac caacggagtt ctcctgccga
     6361 agtttgccaa ggaccttttc ggcgcgggga tcaagcggat caacgtgagc ctggacaccc
     6421 tgcggccgga aaagtacgcc gccatcaccc aaatggactg cttccaccag gtatgggacg
     6481 gcatcctcaa ggcccacgag atcgggtttc gacccatcaa gctcaatgcc gtggtcatgc
     6541 gggggatcaa cgacgacgaa atcgaggacc tgggccgcct gaccttccgg taccctttcc
     6601 acgtgcgttt cattgaattc atgccttttc aggccgaagc gggggaccag cgtttcctgt
     6661 ccgcagacga catcctgagc cgactctccc gggtggctcc cctggttgcg gctcccaagg
     6721 acggggacac caacggtccg gccagccact ttcgttttcg ggacgccctg ggaaagatcg
     6781 gcatcatcag ccccgtgagc caccatttct gtcccacctg caaccggttg cgggtgaccg
     6841 ccgacggggg gttgcgcacc tgcctgttct cgacttcgga ggtggacctg aggactcctc
     6901 tgcggggggg ggcctcggaa gagcggatca tggaaatcat tcgtcgagcc atcgtggaga
     6961 agccggagaa gcattccctg gagagcgcgg tcttccgaaa gtgcatcagc cgtcccatgt
     7021 tttccatcgg gggctgatct tgcgcaggac gttcgaggga ggatcgtctc gaaacccggt
     7081 gtcggtcccg ccgcttcccg cttccatcgg gatccggcgt acaacgaagg ccccgcttcc
     7141 cacggcggcc cgtgaggggt ggcgaaagca ggtgagagga gctcccccga ccccggagtc
     7201 gcgcccatgt ggtgcaggct cgcaagggga cctctttccg ccgccgtggg aggccgttgc
     7261 cggcaccgag gaccatgggg atcgcggttt gaaagggtgc gaggttttcc gatccgggcg
     7321 ggaggcctta gggcccgcat ccttcccagg ccagcttggc tgcccccagg agggcggccc
     7381 ggtcgcccag ctcgcttcga aggatcaccg cgtctgcggg atccagcatg gaggaattcc
     7441 gggaaaggct tttgagaaga gggtcgtgga actggtccca ggaagcgctg acccccccgc
     7501 ccaggacggc gtggcgaatc ccgagaacgg tgaaaagacc ggcaagggcc aaacccaggg
     7561 cccaccccat cctggcgaac agggactggg ctgcgggctc tccctgccgg gcgtaccagt
     7621 aaatgcgctc gggggtgagc ttcccctctt cccaatcctt tcgagcgccc tgacttacgg
     7681 aaccgccctc ggccatggcc gtctcgaacc ctcgaaccag ggcggacccc gaggaatggg
     7741 cctccaggca tccctggagg ccgcaggcgc acggaggtcc atggggatcc acgatcaagt
     7801 ggccgatttc cccagaaaaa cccaggcggt ccccctgcca gagacggtgg tcgaaaaaga
     7861 ggcaaccccc gacgcccgtg cccagggtga ggcccaccca gttggagatc ccccgtgcgg
     7921 cacccaccca ggcctccccc atcccgaaca cgttggcgtc gttttccatg aacacgggga
     7981 tccccagggc ctcctgcagc tccgtcccca atgggtaatt gcgcatggtg ggcagattcg
     8041 gagaaaagat caccgtgccg cgctccccgt cgatcttccc ggcaaccccc atgcccacgg
     8101 ccgccacgcg ggcgccttct ttgcgggcgt cttccagcag gagccgacat tcttcgacga
     8161 ggcgccggga ggcgacctga ggatccaaca gggggcccat gacgatcttg cgctgatgat
     8221 ggatggtccc gccgggatcc accagggcgg agcgcagatg agtgcccccc aggtctatgc
     8281 cgatgaagcg tttgagtgcc atgattcaag agcctctatg ggatttggtc cccgagtcgt
     8341 gtttcgaggg ttctcctcga tgccgatgag cccgttcgag agttccgtcc ttttccccta
     8401 gcccccctgg ggagggtggg attcccggat caaaaagttc tcgggcacgt agaagacata
     8461 gttgcgggtc agaagatcga actgggtgtc cgtttgtgcc tggaccgctt cgaacaggtc
     8521 tttgagggat tcggcgttgg ttacggcgtg gagaaagaga ttgggattgt agaactcggg
     8581 atggagagga aagagaaagt gacggatatt gccccgccag accacgttca gctcctcccg
     8641 ttcttccagg atcgaccggt agaagcgtcg gagcaaatcc gttcgcagga tgggcgatgg
     8701 ggcgttggtg acgcactcca tgagccccgc caggcggtag acccggcgca ttcgaccctc
     8761 gtctttccgg tttcggaata gacccagaac cgtgtaatga tatcctccgt ccagggtgaa
     8821 atgggtagga acgatggcca cgtgtcggtg gcccgtcgtc ttcagggcct cttccaggtg
     8881 agcctcgaac cgggccgatt ccctgcgggt gcacatcaag gtgaagccgc cgtgccactc
     8941 tccccggcga aattccaggg gcgtcgcggt gatgaggttg tccctgccgg gcaaccggcg
     9001 ggtcatgtcg atgagctcag ccacaaaaca ctcttttccg ttattggggg aaatacgggg
     9061 tcaaatcctc ggcgtattcc gtcaacacct tgtgccctcc gtccccgagg atcttcaccg
     9121 tccggtcaaa atcggccaga tccacccgga ggatcagctg ccagacgtcc tcgtgaccct
     9181 tgaggggaac cgtcaggata ctccgaatgt tgattttcgc ctcctccatc tgtcggccca
     9241 cttgggccag gacctcgctc cggtgccgga ccaggaggga aacccgcttg ctgtcttcgc
     9301 tgatccccag ggccgtgagg aggacctcca tgagatccgt gctggtgatg atccccacca
     9361 gccggtcctg cttcaccacg gggagcgctc cgatctttcg gtcgtgaatg aggagggcgg
     9421 cgcgctcgat ggccatgtcg ggagtggcgg tgacgacgtt tcgagtcatg atgttggcga
     9481 tggtgagctt ttggagaacg taggtgagct catgaacgct cagggtggtg gcgggggaag
     9541 cccaggcctc cttgaggtct cggtcggtca cgattcccaa caacctggct ttcccgtcgg
     9601 tcaccggcaa atgggagatc ttgtgagcgt ccatgatttc ccgagccttg aaaatggaag
     9661 tctctggagt cacggtaacg agattggtct tcatgttcca acccacgtac atggcggcat
     9721 cctcattttt ttgtttccaa ccatagggtg agggtctaga atagaaaaag aagtttgaga
     9781 gtgtcaatgt caaatgacac ggaagccgag aggggtccgc agtgtcctca aagaagccga
     9841 tggacgacag gaagctgctg gaagagttct ccaacgccat cgaactttgg gaagactccc
     9901 cgtattacct gccttcggaa gagctcttcg atatcgagcg cttcctgacg gaccaccaag
     9961 ctcatcgcct gaaaacggac ctcgagcaca tctacggaac tcccctcaac cagaaattct
    10021 ggtcttcctt tctggcttat caccggctaa agaaggtgcg cgacgagatc aagacccagg
    10081 aaatgctcca ggaagaaaga aggcgccgat acctggaagc cgtggaagag gaaaaaaaga
    10141 cctggcagaa gtggctcaaa gactggtttc attaccagca aaggcagaag cttcgaatcc
    10201 tccccggcgg aaagaagaac gatccttgac atgacacgac tctgcccgaa tcgagctttt
    10261 gcatgatcgt tccctctccg tcttcggcgt tctccttgtt gcgtttctcg tgattttttc
    10321 ttgctttacg gtgaaaatcg tcattcgggc ttcgtcacca actcccacac gaacagacca
    10381 atcggaggga atccgttttc ctctgcattg gcacaaaggg aacgacttcg tcaagccccg
    10441 ggaaccacac tgcggcattg tgtcgggatc ggaagtgcgg cgcatgatcc tgcatcagac
    10501 tcatgaggac cctccggggc gctttgtcaa tgggctgccg acagtcccct catcgcccaa
    10561 aaccttccgg attaacccac cccgctcaga cagcgatacg agcggggcaa acttcatcaa
    10621 ttcttcgacg tcggtgtctc gagatcgttg acacattcca gtctatgggc ttcgtccatc
    10681 accttggtcc tgtccatggt tccatagcag atagagcccc aagggttcag cgatcagtgc
    10741 atcaaactgt gatttttctc taccccgtgt aggacaggaa aatataatcc gtgcttgtcc
    10801 tgcaaaaccc tagcttgtaa cctattgctc gtgggagaag cgttaagaat cgctgcattc
    10861 tcccgcgacc attggacagg ctcgtacggt gtttggatcc catcaacaaa tcgtcccgga
    10921 aacggcacac ggcctttctc attcaaccac gactcacatt tcgggcaggt atgtctccat
    10981 gaatgcaggt tccatcgtca aattccgcaa ccgggagtgg gttctgcttc ccagtgattc
    11041 gcccgaagtg atcaggcttc ggccgttaac cggggtcacc gatgacgtcg tgaaagttca
    11101 ccgcaggctc tccaatttgc tggggtattc gtttcccgaa gaacgcgtct cgccagccac
    11161 atttccgctt ccccacgggg atgcgattgc cgatgcagcc agtacccatc ttctttggca
    11221 ggcgacgcgc ctcacgttgc gtgaagccgc agcccctttc cgatctcttg gccgaatttc
    11281 catccggcct cgcacgtacc agttcgttcc cctcctcatg gggctccgta tcgacccggt
    11341 gcggctgctc atcgccgacg acgtcggggt gggaaagacc atcgaggccc tcctgattgc
    11401 ccgcgaaatg cttgatcgcg gagaaatcaa acgcttttgc gtcctctgcc cgccatacct
    11461 ctgcgatcag tggcaaaagg agcttgccga gaagttcaat ctcgatgcgg ttctcatacg
    11521 gtcgggtacc gtggcgcaac tggatcgggc gaagactcga tccgaaagca tctatcgcta
    11581 ctacccaacc caggtgatca gtatcgattt cgtgaagagc gaccgtaacc ggcacgcctt
    11641 ccttctagac tgccccgagc tggtcatcgt ggatgaagct cacggcgttg ccgtagccgg
    11701 agaaggcctt ttcagtcaac accagcgcca tgagctggta caggaaattg ccgcgaagcc
    11761 ggaccggcat ctcctgctac tgacagctac gccacatagc ggcatcgact cggcctttcg
    11821 gtccctcctg gaacttctgg atccgcagtt ctcggcctgg atcccctccg cattaaacga
    11881 agcccagcgc tcgaggctgg ccgggcattt tgtacagcgc acgcgtcggg atatcgagaa
    11941 gaactgggaa ggacagcact gtttccctca acgcgatcca tccgatgaga cctatcgcct
    12001 ttcatcggag tacagcgact tgttcaacaa gacctatgaa ttctgccggg agatggtccg
    12061 ttccggttcg cgactggcga aaccccagca acgggtgcgc tactgggggg ctctcgccct
    12121 cttgcgatgt gtcatgtcga gccctgccgc cgctctggca gctcttgcag gccgatacca
    12181 cggcctcccc ggcacagagg atgaaattga ctttcgaacc tttgttttcg aaagctctga
    12241 tgatcgaacc gacgacaatc aacccacccc ccctgtccaa tcggccgagg agactttgaa
    12301 ctaccgtgac cgtcgtcggc tgcgggggct gggacgtcac gccgcggcac tccaacacaa
    12361 gccgggagac accaagctca ccacttgcat ctccttggtg cgtcgattgc tgaacgaagg
    12421 ttaccgtccg atcctctggt gccgctacat cgctacagcc gactatctgg cggagggtct
    12481 gactcgatcg ctggctaatg agtttgacgg cctccgggtt gtctcgatca cgggaaatct
    12541 cacgcacgag gagcgacggg ccaaggtcga agaactgctc catgaaaggg tgcgtgtcct
    12601 ggtggcaaca gattgcctga gcgaaggcat caacctccag cagggcttca atgcgactct
    12661 ccactacgat ctgccctgga acccgaaccg acttgaacag cgtgaaggac gcgtcgaccg
    12721 ctacggccag accgcaccga cggtcaagac catccgttac ttcagccccg acagccccgt
    12781 ggacggggtg gtcctcgaag tcctgctcga caaagcacgg gaaattcacc gtgtcctggg
    12841 aacgcatgtc ccggttcctg aagagagcga aacggttacg gaagcagtgt tgaatgccct
    12901 ttttctgcgg gggcgtcccg cacccaatga gccgtccaaa gacggtccga tccagctcac
    12961 catgtggccc aaggtggcct ccttccaccg caaatgggac ctggacgccg agagggaaaa
    13021 ggtcaaccgg actcgcttcg cacagcggac gctgaaaccg gaggaggtgc gacgagagct
    13081 tgaagcaacc gacgcggttc tgggggatcc tgaggccgtg cggcttttcg ttctcaatgc
    13141 cgctcagcgt ctggggcttt ccattaccga ggacagacgc atccccgatg cctatcgcgt
    13201 ggccacaggc cccgaaattc gcgccatcct ccccgatgcg gtttccttcg cacttcccga
    13261 ttcaagggtc gctcaatggc ttatcagttt cgtttccccg actccagaag gtgctgaata
    13321 tcttggacgc aatcatcggt ttgtcacggc cctggcccag tttctcctgg aggaggcatt
    13381 ggtcaagggt gcagatgccc gtgccgcacg cagcggtgtg attcgcacaa ctgcggtccc
    13441 gcgtctcacg acgctctatc tgattcgcgt acgatacctc ctggagcagc cggaccggct
    13501 tcccgccttg gccgaggagg tacaggtcct aggccggcga atggagaagg atggaaagga
    13561 aatgtggctc tcgcccgacg aagccctgtt gctgctttcc gcggcaaaat ccgaggccat
    13621 cgtttccatg gtcgaaaaac gagaactggt ctccatggcc ctggatgagt gggagcgcat
    13681 catgcctgcc ctccgtcggc gtctggacga gcgtgccgcc gagctcctcg atagccaccg
    13741 ccgtgtacgc cgggccgtcg ccatgaaggt tcgtggtctt gccttcaaac cacagctgcc
    13801 tccggatttg ttggggatgc tgatcttgca gcccatggtg aaactatgag cagcaaccga
    13861 tctatcccct ggtatcacct tttcgaaatg cactggatgc cgggtgatct caagcgatct
    13921 taggatgagg cgagggactg tgatatggtt attttgcctc cataccaccc ggcgagattc
    13981 cgtaaggtga tgaagacctg gaaaaagggc ggtccataga tcatgggagg gcgcagagcg
    14041 agcagtggac tccggctgtc cccatagccg ttgcgccttc agtccctggc tcctgagaag
    14101 gagaactcga tgctcacgcg attccgtttg gagaatttca gaagctacca tggcgcgact
    14161 ctggatcttt cgccgctcac gctgatggtc ggcgcgaatg cctcagggaa gagcaacgcc
    14221 ctggaagctc tgcaaatgct gtcctggcta tctcgtggac gaagattgga ggatgtattc
    14281 cgtgccgtgg aacagatgga gctactcatt cgcggtaggg taacggatct gacgtacgaa
    14341 ggcgcacaga ctttccgtct tggttgctcg ctgcagcaag caggaccatg gagggatctc
    14401 gatgtggaga tcgctgtgga taaggaaggc atgcggattc gacaagagag tatcaccagt
    14461 cctaccgaac gttcgcccct ctacaaagta gaacagccgg ctaccggctt ttctcacgac
    14521 attcttgttt cttacaacaa cttcgcacga gggggcatca agccccagat cagctgcacc
    14581 gatcaacagg gtgtcttcac tcagctattg acgccggctc gtttcgcgaa ccatcacaca
    14641 cgctctcaga aggaaattcc gtcgaccgcg aaagtattca tccaagctct ggaacggata
    14701 ctctttctcg atcccgtgcc tcgacgaatg cgagaaccca gctacgtctt cgacaaggct
    14761 ctcaaaggag atggaagcaa catctccagc acacttcatg acctgtgcgt cggtcaacag
    14821 gcaaaggaaa aggtcctgga tttcatccgc gaccttcccg aacaagacat cgcagatctt
    14881 caattcttgg aaggaccgcg tcaggacgtc atggttcagc tcgaggaaac gttcggtggg
    14941 accggcaagt tgcgagaagc tgcccttctc tccgacggca ccttgcgtgt gttggcagtg
    15001 gctgcagcac tcctatccgc acctgcgggc tccatggtag tagtggaaga gattgacaac
    15061 ggcgtgcatc cgagtcgggc aagcaggctg ctggcaaata tcgaaagcgt agctcgcgaa
    15121 aagaacctag ccgtgctggt gaccacgcat aatcccgcct tgctcgatgc attgccggat
    15181 ggagccgttc caaacgtggt gttctgttat cgtgatcccc aaaaaggaga tagtcgactg
    15241 gttcgcctga gcgagatccc tgatttcccg gatcttgtgg cacaggggcc actcggacgc
    15301 ctgctcactc agggcatcat agaccgcttt gtcaaaacgg cccggtcacc ggaagagaag
    15361 gtccgggctg gcctggagtg gatccataac ctgcgtaaga cggcggcatc atgagcatct
    15421 gcctgatcga cacgagcgtc ttctgcaatg tggtggatgt ccccgggcgc aaccagcaaa
    15481 gaggtgaagt gctaaacagc ctggagatgt tcatccgtca aaaagcctct ctccttctcc
    15541 ccgttgtcgt tctcctcgag acggggaatc atatcggcca tgtttcggat ggtgggttgc
    15601 gaaggctcgc ggctgaacgg tttgttgaac aggtggcggc agctctggat gggagggctc
    15661 cctggaagcc gacgcccttt gtagaagccg atcagttgcg aatctggctc aaggaattcc
    15721 ctgaccatgc agggaggggc gccggtctcg gagatctttc catcatcaag gaatgggaac
    15781 ggcagcgcag gctcaacccc aatcgcaggg tcttcatttg ggcgttcgat aaacacctga
    15841 gctcatacga cacccaatgc tagccacagt ccttggaacg agtctctgac gatgagtttt
    15901 ccatccgttc gcatcgaagg tgggctgttg ggtcccgaca ttctggacct gctggtgagt
    15961 ggtgatcttg ccggtcagaa acccgtggac ttcggtcttg aagcacgccg caacctcacg
    16021 gatgaaatag ccgccacctt cgccgacgct cgcgccttgt ggcgcatttt tcagcatcgt
    16081 ctggagcggc tggctcccga tgaccccgcc accagcgtca ctcgtgatgc ctggatgatt
    16141 ccgttcctgg gtttgctcgg ctacgagctg caatacaacc cgcgagccta tgacgtggaa
    16201 gggctgacct tcgccatttc ccatcgagcg gttagttccg ctgcgggcgc ccaaacgggt
    16261 gcggccgaag tcgtcgatgg acccccggtc cacatcgtcg gtgcacgcca ggaactgggg
    16321 aagctcgctt cgagcggtcg gccccggctt gcgcctcact ccctggttca ggaatacttg
    16381 aaccgcacgg aatttgtctg gggtatcgtc accaacggtc tcaccctgcg gcttttgcgg
    16441 gattcgactt ttgtccgccg tcaggcctac gtggagatcg acctcacggg gatgttcgag
    16501 gagcaacgct tcggcgattt cgctgccttc tatcggctgc tccaccgcac ccgactgccc
    16561 catggtcctg ccgatgccgc aacctgcttc ctcgagcaat accatacacg gtcgctggtc
    16621 gagggcagcc gcatacgcga gcacttacgg gatggcgttc aacgctgcat cgaaatcctt
    16681 gcagaagggt ttctcagcca tccagccaac gaagacctcc gccgggaaat cgaggaaggc
    16741 cgcctcaccc cggaggacct ttatcggcaa ctcctgaggc tcatctatcg attcctgttt
    16801 cttctggtcg ccgaggatcg tgggctcatc agccaaaatg cgctctattg cgagtactac
    16861 ggcatcggtc gcctgagaaa tcttttcgat agacgcggtg ccttctccga ccatgaagac
    16921 ctttggtaca gcctgagagt cctgtggcac gtgttgagtg atgagcggct ttcttccatc
    16981 ctcgacgttg cgccgctcaa tggagagctt tttcggtcgc agactctgga cgactgcatt
    17041 ctcaccaatc gaaacctact cgaagccttc cggcatctgg cctggtatcg cgaaaaccgg
    17101 accgcgcagc cacgacgtgt gaactactcc gccctcgacg tcgaagagct ggggtcggtc
    17161 tacgagagtc ttttggaact ccatccccac ttggacgcat cgatcgtccc cccccgcttt
    17221 gaactgatcg tttccgggag ggaacggcgc tcgaccggat cccattacac gcctcccgag
    17281 ctggttgctc ccctcatcga gaatgcgttg gggccggtca ttcaggaaaa actgggggag
    17341 gcgaagcgga tagcgaatgg cgagtggcga acggaacagg agcggaacca tgcgcttgct
    17401 gttgtgtgga gagagcgagt tgaggacggc gaatggcgag tggtgaatgg tgagtggagg
    17461 agcaaggatg gtcgagtcgt atcgcgatct cgatgtgtgg aagcgggcca tgacattggc
    17521 gaaagaggcg tacaggttga cgacctcgtt gccgaaggaa gagctttatg ggctgatctc
    17581 gcagattcgg cgtgcggcaa gctccattcc ggcgaacatc gccgaaggct ggggcaggaa
    17641 ataccctgcg gaattcatcc agttcctgag gaaggcaaac ggcagccgca ccgagctgga
    17701 gacacatctc ctgttggccg cggagattca gctttgccgg atggaacaga tcgacctgct
    17761 cctgagagaa ctggagatcc tcggcaagca gatcctgagc ctggagagga gtctcgctcg
    17821 ccgcaccaaa acctaacctc catcgaccag ttcacacaac cggccactca ccactcgcca
    17881 ttcgccactc acccctcgct actcacccac tggaaatcca ccccattcgc cactcgctac
    17941 tcactactcg cccaaaaagc ccttctcacc ctccgagttt gcgatcccgc ctgcggatcg
    18001 gggcactttc tcctcgcggc ggcccgccgc atcggaaaag agctggcccg cgtgcgcacg
    18061 ggtgagcaag agccggctcc cgagggtgtt cgcgaagcgg tgcgggaagt catccacaat
    18121 tgcatttatg gagtcgataa gaaccctctg gcagtggacc tgtgtcgtgt ggccctctgg
    18181 atcgagggcc acaccacggg caaacccctc accttcctcg atcaacgcat ttgctgcggc
    18241 gattcgctgg taggcgtatt ggagctcaaa gaccttgatg gcggtattcc cgatgcggcc
    18301 ttcaagcccg tgaccggcga cgacaggact gctgcgcgtg aggcaaagag acgaaatgca
    18361 gccgctcgcg gcgccaccct cttccagttt ccctccagcc gccgcctgca ggtcctcgcc
    18421 caacaactcc ggtcgctcgg ccacctcccc gaagacacca tggaacaggt gcgcgacaag
    18481 gcggaaagct tttcacgcct ggtgcaaagc gacgactatc agcggctgaa gttggcttgc
    18541 gatgtctgga gcgctgcgtt ctttcaaccg tttccaggtc cgtcagccat gcctgtcacg
    18601 acagccgatg tgcagatggc gttgatgaaa gggcgattat cggatccacg aatgaccgcg
    18661 ttcgtgcagg aaacggcagc gaagcaacgc ttcttccatt ggccgctcgc ttttccagag
    18721 gtgttcgggg gtgggggttt tgacgccatc attggtaatc cgcctttcct ggggggactc
    18781 agagtgagtg gtgagtatgg cctcaaatac cgccagtggc tgtcgattgc ctttgaacct
    18841 ttcacgggaa cagccgactt gtgcgcagcg ttcttccgac gctcctttca cacaatccgg
    18901 cctgaagcac gattcggcct cattgccacc aataccatcg gccagggcga cacgcgtgaa
    18961 agtggactcg ctcctatcgt gagtaaagct gggtccattg tctttgcccg gcgtttcgtc
    19021 aagtggccgg gtgcagccaa cgtcgaggtc aacctcgtag ccgtgcgcaa aggcccatcg
    19081 gaaagcgaat tccgactgga cgaccagctt gtgccgagca tttcatcacg attggacgat
    19141 gaaccggaag ccgaacctca tgccttgcga cagaatcaag gtaaagcttt tcaaggcgac
    19201 atcgtacgag gcattgggtt tgtcttggag ccaggcgaag ccagtcaaat ccttcatcga
    19261 gacccgcaga acggcgactg cctgcttccc tatctgaacg gcgaagacct caatacgcat
    19321 ccccaacaag agcccagccg ttatgtgatc tgttttcatg attggcctct gaaaagagca
    19381 gagcagtacc ctgacctttt gaggattgtg gaggaacgcg tcagaccgga gcgcgagcgc
    19441 ctcaggaacc gggatgatcg gcacgatcgc gaatactggt ggctattcgc ccgctatcgc
    19501 agtgacatgc gcccggcaat tggtccattg agccgggtga tggcgcgaag ccgcgtgagc
    19561 gagttacacg ctgttagttt ggtgccaaag ggttggatct gtaatgaaca gactgtggtc
    19621 ttcgccttcg aggacttctt tcattttgcc ctaatccagt caaatatcca tgaagcgtgg
    19681 ttgcgacgaa acgcctcgac catgcgcacc gacatccgct acaccccgac ggattgcttc
    19741 gagacgtttg ctttccccca gaatccttca gaggcatcaa gatcattcgc agagcagctt
    19801 ggtgcctcct atcatgctca ccgccgtcag atcatgctcg accgccaaat cggtctcacc
    19861 aagacctaca acctcttcca caatccccaa gccaccgacg cggatatcct ccggctgagg
    19921 gagctccatg cggaaatgga catggccgtt cttgcctgct atggctggga taatctcgac
    19981 ctagaacacg gctttcacga aaacgaacgc ggtcaaaccc gatacaccat tagctcttcc
    20041 gcacgccgtg accttctccg gcggcttctc gccctcaatc tcgatctttt cgatcaggag
    20101 acgagggaaa ggaatccaga gtaacttgtc cacgattgag gaggaccggc catggtggaa
    20161 cccgccaaga agagagccac ctatgaagat ctttccaacc ttccggaaaa catggtcgga
    20221 gagatcattg acggcgagct ggttgtcacg ccgaggcctg ccaggagaca tatcgatgca
    20281 gccgcgtccc tgggcgggga gctcatcccg acctaccgct tcggtcgcgg gggacccggt
    20341 ggatggatca tttaccacga gcctgagatc catctgggag aagacgtcct ggtgcccgac
    20401 ctggcgggat ggagaaaaga gagactgaca acggctcccg aagagcaccg cttcgttgtg
    20461 gcccccgact ggatctgcga aatcctgtcc ccgagcacga tccgggtcga cagggtcaag
    20521 aagatgcgca tctacgccgc taatggagtc cctcacgtct ggatcatcga tcccgctgcc
    20581 aaaacgctcg atgtttttag gctcgagtcc ggcaaatggc ttttgctcga cacctttgtg
    20641 gaagatgaca gggtacgggc cgagcccttt caagaaatcg agatcgagct gggcaatttc
    20701 tgggtcgccg aaagctgaca gccgtatcga atccgttcat cgagtcatcc aggcctcttt
    20761 gcgtatcatc cgcagggcaa agggaagtgg atcggcttca gggaaaagat ggttcgttca
    20821 ctcgaggaga gccatgagca tctttgacct tcacgccaac gtcatcgccg attaccgcaa
    20881 cttcgtccgc gcgttcatca cggtggccga cgatcgggtg cggggctttc tggatcgggc
    20941 gatggacgaa gaacagcgac tctggccgga gtccctgctt cagttgagcc catcgtatga
    21001 tcgggttgcg acggttgcgg agttggcggc gcaaggcgtg ctccacgagg agaccgcacg
    21061 cattttctgc acgcccgacg gcagaccgtt ccacctttac cggcatcagg tcgaggcgct
    21121 ggagaaggcg agggaagggg agagctatgt tgtcacaagc ggcacgggat cgggaaagag
    21181 cctgacctac tttctcccca ttatcgatgc gctcatgcgg cgccccgcga cgggagagcg
    21241 ggtggcagcc ctggtggtct atcccatgaa tgccctggtc aactcgcagg tgcaggctct
    21301 ggagaagctg cgggaaagct atcagcgaag gacgggactc ccgtttcccg tgacctttgc
    21361 gaagtacacc ggcgaaaccg ccgaggcggt ccgtggtgac cttcgagcgc atccgcctca
    21421 gatcatgctg acgaactatg tgatgggtga gctcctcctg gtgcggcccg aggatcagcg
    21481 attcctggac cgtgcgggcg ggggacttcg cttcctcgtt ttcgatgagc tgcacaccta
    21541 ccgaggccgg cagggcgcag acgtcgcgat gctgatccgt cgcatcaagg agcgctgcgc
    21601 cgcgcccgag cttgtttgcg tgggcaccag cgccacgatg gtcgcgaatc gggacgccac
    21661 cccccaggag cggcgtgcct ccgtggcgga cttcgcatcg aggctttttg ggatctccct
    21721 cacatcggaa caggtcatcg aagaaacgtt ggtgcctgtt accgaaggcg gttctccatc
    21781 gcatcgagag ctggtcgaag gtctttctgc tcctcttccg gaggacctcc ccgagctgcg
    21841 ccgtcaccct ctggcccgtt gggctgaggc cgaattcggc gtcgagcccg aagctgaagg
    21901 gcggctcaaa cgccgtgtgc ctcgccctct ttccgaggcg gcgcgtcgcc tggccgagga
    21961 gaccggagcg gattttgagg tttgccggga gcgactccga gaaatcctga tccgaggcag
    22021 cgagatcccg agtgaggagg gcacccgtgc tttcgccttc aagcttcacc agttcgtgag
    22081 ccaggggcgg gccgtgtatg ccaccatcga gtcgcgggat cgccgcgagc tctccctcga
    22141 tgggcagctc gcagccagcc agggacgtgt gtttttcccc atcaagttct gtcgccagtg
    22201 cggacaggaa tactatcacg tgctgggggg cggcccgggg gagccctttc ggccccatcc
    22261 catcgggacc gaatcggtgg atgtgaatgc ccgcgtgggc tacctgatgc cggcctcgga
    22321 agtggatgac tggaatcccg gccggatccc cgtggagtgg ctcgatcgga gagggcgact
    22381 caagcccaca tggaaagaga gggtaccgat cgaggtatgg gtggggccgg atgggacacg
    22441 ttacgaccgc gcccatcagg gagccgtgag gatgtggctt cagtcgcagc tcttttcgct
    22501 ctgcctttcc tgcggggaat tctacacggc gcgagaacgg gaattcacca agctcgccgg
    22561 cgcttcgagc gaggcaagaa gcagcgccac gacggtgctt gcttcttcac tgttgcgaca
    22621 tgccgaggaa gagggcggcg ctcgcgacaa attgctcacc ttcacggaca accggcagga
    22681 cgcctctctt caggccggcc atttcaacga tttcgtccac gtatctttgc tgcgctccgc
    22741 tttgtatacg gcgttggtca aaaatcagca gctcgccttc gatagtgtcg ccgaggcaac
    22801 ggtccgtgaa accggcctca tgattcggga catcgcgagg aacgcggagc tcgacccgga
    22861 ttctcccgca gcccgcgatg tgtggcaggc cttcaccgag cttacggaat atcgactcta
    22921 cgaagacctg cggcggggat ggcgcgtggt gcagcccaac ctggagcatg tcggtttgct
    22981 ccgggtgggt tatcgaggcc tcgaggatct ttgcagcgag gatcgacgcc ggcaatttca
    23041 tccggcggcg gcgggggcat cgagtgagga gtggttcttt ctgacacggg ccgtgctgga
    23101 tctgttccga cgaaagctgg cgatcgccgc tcgctgcttg atggaaactc agcagcaaca
    23161 gattcggcga cgttcggaac agcacctgaa tgaattttgg ggactggacc ctgcaggggg
    23221 tgatctcagg gcggccaacc gtttcgtacg cctcggccgg tcgagccgca ccgtggaggg
    23281 attcagtctc aatatccgaa gtacccttgg gcgtttcatg tccgagagac tggggttgga
    23341 cgcgacggac tacgatctgt ttcttgacgg attcctcgca cttctggtca gtctcgggtt
    23401 tctggtacgg cttgaaccgg tggacgatca ccagttctac cagttggacg cctcgtgttt
    23461 gctctggcag gtcggtgatg gaagtccgcc gccaccggat cccatctatt cctgcagagc
    23521 gagtgggacc ggatacacgg atgccgcacc accggtaaac gcctttttcc gtgatttcta
    23581 tcgcggtgaa gccgctcgat tggcctccct tgaggcccgc gagcacacag cgcaggtggt
    23641 ccggtcgggt gagcgggaac gccgagagcg acggtttcgc tgggaagagg gagacgtcca
    23701 gaggcgggag ttggggcggc gcttgcccta cctggtttgc tctccaacca tggagtt
//
LOCUS       DAOOVC010000051        23740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1457_length_23740_cov_7.015411, whole genome shotgun sequence.
ACCESSION   DAOOVC010000051 DAOOVC010000000
VERSION     DAOOVC010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 23740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23740
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1457_length_23740_cov_7.015411"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLM79_08565"
     CDS             <1..437
                     /locus_tag="PLM79_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86401.1"
                     /translation="VREGQPCEIRLDSIPDERFTGTVFTVVPTADRTKGTVLVKVRFD
                     ALDPRILPDMSAKVAFLTRPLASGELQPRLGIHRDALTQRDGISGVFAVDREVVRWVP
                     ISSPTTLGDFVLVGDRVPQGTKVVLKPPDALKQGGKIQISES"
     gene            615..1325
                     /locus_tag="PLM79_08570"
     CDS             615..1325
                     /locus_tag="PLM79_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86402.1"
                     /translation="MEPPQSPEPMVILEGIGKSYRRGVQKIPVLTDIDLTIGTGEMTA
                     LMGPSGSGKTTLLNLIAGIDHPDEGVLIVAGTRLGDLTEVELARWRAVHVGFIFQFYN
                     LLPVLTALENVELPLLLRDLDRRQRREHAALALELVGLPDRMNHYPRQLSGGQQQRVA
                     IARAIVADPTLLVADEPTGDLDKHSAGEVMELLEQLNRDFGKTILIVTHDPKTAKRAR
                     VIHHLDKGVLVRSENSRE"
     gene            1350..2507
                     /locus_tag="PLM79_08575"
     CDS             1350..2507
                     /locus_tag="PLM79_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOV86403.1"
                     /translation="MELLKLVVRNTQRQRLRTALTVLGMAVAILAFCLLRTVVEAWYA
                     GVEAASPNRLVCRNAVSIIFTLPLSYRQKILQVEGVTQVAYANWFGGIYIDERHFFPR
                     MAVGPEVFFDLFPEILMPEEQLKAFHRQRNACIAGRKLIEEYGWKLGDTITLTGNIYP
                     GEWRFVLRGIYRGATKTVDETQFFFHWDYLDESLKKQSSGRSGMVGWYAVQIRDPSRA
                     GAISQAIDAMFKNSWAETLTETEKAFQVGFVAMTEAIVTAIRVVSFVVIGVILVILAN
                     TMAMTARERLWEYAVLKTLGFGNGFLWVLIAGESVSISLVGGLLGTLLSFPIAGVFAQ
                     RLGTLLPIFHISLQTILLNFVVSIAIGLIAALLPAWRACRVKIAEALTHVG"
     gene            2591..3598
                     /locus_tag="PLM79_08580"
     CDS             2591..3598
                     /locus_tag="PLM79_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOV86404.1"
                     /translation="MNPIRRSPEKDPGIHLLVGFRGQTFEEELKALVHEYCIGGIVLF
                     KRNIEAPEQLRCLLEDAQAFARESLGRRLWVAVDQEGGPVQRLGPGFFQLPSAHELGV
                     AGARSVKEWGKKAAMDLRRMGIQINLAPVLDLVGRDSTHFMEARSLGWDSEQVADLGK
                     LWIRVLQENGVSATAKHFPGLGRAESDPHHYAPVIHWADEEDMNRDLHPFREAIRAGV
                     HCVMTSHALYPLLDPRWPATLSPQINGEWLRGKLGFQGVLLSDDMDMAAVSERYSVEE
                     IARQGFASTLDFFLSCQVSENIEPLARALADGCRRDPVMAQAHQSSLERLERLKKRHD
                     G"
     gene            3588..6755
                     /locus_tag="PLM79_08585"
     CDS             3588..6755
                     /locus_tag="PLM79_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86405.1"
                     /translation="MMANGSPPGIPPGGARERVSGRRRTAAVFLAAILTVLGISAGNS
                     SGQSRQVLILHSYHSGLTWTDSVMDGIKRGFSESKIDVQLSAEYLDARRYPDPASSKP
                     FRDLILLKLQTLTPDLILVSDNSALAFLLEFRQRLFPQVPVVFCGINDFHPSMIMEHR
                     QITGVAEEISVLETVQLAMRLQPETREIIVIGRTSVPADKANRDSFTAALSDLPSQLK
                     VRFWDDFPLQELKTKLEELRPGSVVFINGLLQDETGHQLMYGETTRWISRHSPVPAYS
                     FWDVYLGHGIVGGKLVSGYHQGKTAAQLGLRILKGESADHIPVITGGQANLYMFDYRQ
                     LERFHIPSSALPDNAKVLHLPEAFYHRHKKLVWVTGAIIATLSLFVVLLASSILRRRR
                     AEAALRESEEKYRELVQNANSIILRMDPQGKITFFNEYAESFFGYRQEEILGKSVLET
                     ILPEKEHGGRDLRAVLRDTITHPEDHPVQEHQNIRRNGERVWIAWGNKPIRDDRGDLV
                     EILCVGNDVTERRQAEEALRESELRWQFALEGAGDGLWDWNTRTDEVYFSPQWKAMLG
                     YGEEEIPHRLSEWDRRVHPEDKEYVYREIERHFSGQTPLYTSEHRLLCKDGTYKWILD
                     RGKVIRRDEDGKPLRMIGTHTDMTERRRGESDRIEMERQLLYAQKLESLGVMAGGIAH
                     DFNNLLMVILGNLDLALHEIPSQSSAAQSIEQAILASRRAADLTLQMLAYSGKGRYFL
                     EDFSVGELIRENAHLLRASISKHSALVVQTEANLPHIRADRGQVQQVLMNLITNASEA
                     MGEKGGAIRVHAGVGDFDRAELARSKTDEKPPPGPYVFVEVRDTGCGMNEETLRRLFE
                     PFFTTKFLGRGLGMAAVLGIVRAHHGAIFVDSKNGEGTNVTVLFPVSGEARPAAEEIE
                     ERQKEVSPAVEPSGDVSTRAILVVDDEDLVLEVCGTMLESLGYRILTATNGEEGVKVF
                     EEHAGDILCVFLDLTMPRMDGVRAFHEMRRIRPDVTVILVSGYSQDEATQRFAGEGLA
                     AFLQKPFGIQKLATTLKEALGHERP"
     gene            complement(7148..7837)
                     /locus_tag="PLM79_08590"
     CDS             complement(7148..7837)
                     /locus_tag="PLM79_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HOV86406.1"
                     /translation="MRNCDHRSRWDKLRGIWEIIDRLRGEGGCPWDRKQTPASVQAYL
                     IEEAHEAAAAVRTGSASEAAEELGDLLFMTLFLVHLYEEDRHFTLEEVCDRITEKMIR
                     RHPHVFGDVTAKTAEEVKDNWEKIKADEKSGRGKNPSAVPETLPALMRAYRTVSRLSH
                     REGSPWNDPRRLFEDLRNRMEDLASRIHGGGDPSGRDFAQLLLSLVNLARLQGYRAED
                     CLHDLLRDLDA"
     gene            complement(7824..8414)
                     /locus_tag="PLM79_08595"
     CDS             complement(7824..8414)
                     /locus_tag="PLM79_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOV86407.1"
                     /translation="MNGLDWVIVVVGGICILRGIWRGAVSQVFGIGGVLGGFLLASRN
                     HEALARQIADTLPKVGFAPVLSFLLIFFLTWLCVGMLGYFLGKLLRLTGLGFLDRLLG
                     GTVGFAKAGVLAVILVWLLTLFLPSESPHLRRSTLLPYVHQFARILVMATPQRLQDQF
                     HRKRREIEKYWSDQSREASKPSPGKRGKEKSPHEEL"
     gene            8680..9114
                     /locus_tag="PLM79_08600"
     CDS             8680..9114
                     /locus_tag="PLM79_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOV86408.1"
                     /translation="MYREIRKGRTIMGRLAKGVDLLAALEQVCRETGVNLGELTAIGA
                     VSKARVGYYDQERRVYAFEQWDRPLEILSLAGNVSLKDGGPFVHAHVTLADDRGRGFG
                     GHLAEGTAVFACEFVLREYLSEKEYVRAPDADTGLSLWKDAG"
     gene            complement(9270..9668)
                     /locus_tag="PLM79_08605"
     CDS             complement(9270..9668)
                     /locus_tag="PLM79_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV86409.1"
                     /translation="MEPAGEEKRSFTRIPFQSEAIVKCSQKTLVCDLENLSLNGLLLK
                     TPEPISVNEPVELEILLSGSSSELSISLQGIVVRQAEEGVAVQFRKMDLDSFIHLKNV
                     VSYNCGGQADVMEEFSRYIERKREEQGENS"
     gene            10037..11374
                     /locus_tag="PLM79_08610"
     CDS             10037..11374
                     /locus_tag="PLM79_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV86410.1"
                     /translation="MKRSSDRDDPVQKISINGLSEREFLSVYNAGKVLKLNVGDNLVK
                     EGTPGDLLFLVLKGNLAVCEEFPGDSGDVVFREGDWIGEGAVPEPLDGRGFLTARKPS
                     LVLALDPSALENLDPGVRNFVWRKLHKGLLARIRSLEKSSRDLFSSSRRQGMQMRELL
                     RKRTRGYEQSEIILSILKNVPRLPMHVSKLVQVLSQEGTSAREVTQLAKEDPSLVGEI
                     LKTVNSSYYGLQKRISDLSYAIVYLGFNQVYQIVIANGLRKAMPRTHRFEELYEHSVV
                     IAHLAFEIGQLYDRRKSSTMGTIALLHEIGESVVLLLKAQNPRWSFFIDMLEPSKVAA
                     MLLKDWNIPDNICETVEYQYYPEFVPPGDIPTPYKDCVAVLYLAHAACDCIGGGAEGI
                     LRQPYLKDYLQFLDMPEATVSGLLDKYVLTDLNMKIHTLPKHVQSFILKRKGG"
     gene            11518..12180
                     /locus_tag="PLM79_08615"
     CDS             11518..12180
                     /locus_tag="PLM79_08615"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOV86411.1"
                     /translation="MNWMGLLVALLISVMTLQVAPVYAEGMQDDVDQAVTIIKRFQDI
                     PEKSIPRTVLRDARGIAVLTVIKAGFIVSGRGGTGVVVARTKKGWSGPSAIGTGGAGF
                     GLQIGAQVSEFVFILNTDEAVRAFSREGNVTLGTDLSVAAGPIGRSLGVGVTPVAAVY
                     TYSRSQGLFAGVSLEGTVIGTRNDANTKYYGKPVSPHDILTGRVKAPAGARALQKTLA
                     KY"
     gene            complement(12258..12593)
                     /locus_tag="PLM79_08620"
     CDS             complement(12258..12593)
                     /locus_tag="PLM79_08620"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV86412.1"
                     /translation="MDRRSSKRTRKVIRIQWKNEDVVHDGVTLDICPGGIFVITSQKV
                     PPSSQIHLQLWLGKDSPLSCRGRVTWVNRGQVVYYPPGFGVQFLDLPEESRKILDPIC
                     DGYDPIEAV"
     gene            complement(12887..13081)
                     /locus_tag="PLM79_08625"
     CDS             complement(12887..13081)
                     /locus_tag="PLM79_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86413.1"
                     /translation="MRLRFLREGNSFGIICAHEQLDRMLKVIRHNGGSGSIQRRGRDA
                     VYVRVSKGPEPSSRPGPAPS"
     gene            complement(13153..15084)
                     /locus_tag="PLM79_08630"
     CDS             complement(13153..15084)
                     /locus_tag="PLM79_08630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV86414.1"
                     /translation="MADISAQPTVFDREGSNARPWQSMYQAKKTTAAKALRKIKRGNR
                     VFIGSGCGEPQHLVRALEEIVPLLADVEILHILSVGRSGYTEASFFDKCRLKSFFVAA
                     ASREAVAEGRADYTPINLGDIPDLFRTGAVRVDIALIQVSPPDQHGFCSYGIAVDIVK
                     AAAENAKFIIAQVNPQMPRTLGDSFIHLRDLHAIVEHEEPLLEVGQPLMNPIAEDIGR
                     HVAKLIEDGSTIRVGVGSISTAVLYALHDKKDLGVHADMLTDTYMYLAKKGVITNARK
                     TLHPGKIVASFCLGSRELYDFVHNNPMVAMYPIDYTNNYMVISENDKMVSINSALEVD
                     LTGQVCADSVGYEIYSGVGGTIDFLRGARHSYRGKAIVVLPSTTLDGQRSRIVRSLTE
                     GGGVVTTRGGVQYVVTEYGIAQLHGKSIRERALALIGIAHPDFREELMRAAYEFNYLR
                     QEAFPVIAPKAVYPAEHETSQLFDTDLTVFFRPAKPTDERALKEFFYSLPREEAYVRF
                     LSNMRVYPHYDVQRMVNIDYDRELTLFGLVGEVDAERIIAIARYLLKEENLEGEIDFA
                     VHPDFGKKGIASFLVHHLMRIARRKKIRTLVAYSTLGNEKVLGVFQRSGYLMETSFSR
                     GVYEIRVDLTREAEVCLTD"
     gene            complement(15114..15917)
                     /gene="lgt"
                     /locus_tag="PLM79_08635"
     CDS             complement(15114..15917)
                     /gene="lgt"
                     /locus_tag="PLM79_08635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOV86415.1"
                     /translation="MIPYPQIDPDLVTIGPIHIRWYGVMYVLGFVAAYFFIQRQERSR
                     QVGLVGVVAQDLVFYLAIGLIVGARLGYLVFYQYHSYLFYLENPLEIIATWRGGMSFH
                     GGLVGAVLAGLIFCRRRNLPFWVIADSAAVTVPVGLGLGRIGNFINGELLGRPTQVPW
                     AMVFPGGGPVPRHPSQLYEAFLEGLVLFLLLWVLRKKDFRDGMMVVFFLVFYGAFRFF
                     LEFFREPDPQIGFLAGLFTMGQVLCLSMIAAGALLATFLSYRTPQTGQG"
     gene            16242..17699
                     /locus_tag="PLM79_08640"
     CDS             16242..17699
                     /locus_tag="PLM79_08640"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOV86416.1"
                     /translation="MESSATEPFGCLIHGRLEMGAETYPVFNPSTGEVFARVTTADRS
                     MVHRALESARESRGSWQRLPSSERADLLHRLAAKVREQADPLTFLLCNEVGKPTQSAR
                     EEVHHTAALLDFFAEESLRLLGRIPLRGYPGEQVLIVRMPVGVVVAITPFNYPLSTLA
                     CKMAPALGVGCTVVAKPDEHAPLSTLEMAKLALEVGFPPGVLNVVTGPGPEVGPLLVD
                     HPIPGLIAFTGSLEVGRQVQAISARWVRKVVLELGGHCPAFVCHDADWPALLDSLVLQ
                     SLKNSGQYCYRPSQIFVDGRIYEDFEKRFIARAAQCRVGAAWEPDVDLGPLNNRELLA
                     KVDDQVRGAVQEGAHLALGGYVPERPGFFYAPTVLTCVKPQTAIMQEDVFGPVVMLHR
                     AEDMEAAVDQANASPHGLAAYLFTRDLGSAMEWASRIEAGSVWVNRIHQAYPEAPFGG
                     MKQSGLGREKGHAGVEEFTELKTIYLSYGSPPLNK"
     gene            17714..18370
                     /gene="mobB"
                     /locus_tag="PLM79_08645"
     CDS             17714..18370
                     /gene="mobB"
                     /locus_tag="PLM79_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOV86417.1"
                     /translation="MLPIVCVVGASNVGKTTFLEKLIPELTKRGYRVGCLKHDAHSFE
                     MDHPGKDTYRHRQAGARTIAIASPDQVAAIRRVDSEPSPEEMAARLFWDEDILLTEGY
                     KRSNLPKIEVFRSAIESKPICTREDHLVALVTDDAVDVQVPRFRFHEADKVADFIEER
                     FLKHRKKHGVLVQLDGKQLPMKEFVRDFVVGAIRGMLSSLRGWEDPRTVHIHIRLKDR
                     "
     gene            18371..18964
                     /locus_tag="PLM79_08650"
     CDS             18371..18964
                     /locus_tag="PLM79_08650"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HOV86418.1"
                     /translation="MVTGAILAGGRSRRYGRNKALEVFDGLRLIDRGTASLEPYCAPV
                     FVVANDLWEYADVQAVLLRDLIAHQGPLGGIATALWFSPHEWVFVKAADMPFLVPELL
                     TLMLGAKEGFDAVVPVHRELYEPLLALYHRRCLPAVNRVLEGPERKVIAFYRQVKVRF
                     LQEFEWKTVDPKALSFMNANTPEEMAALEWNWKKHGS"
     gene            18937..20196
                     /locus_tag="PLM79_08655"
     CDS             18937..20196
                     /locus_tag="PLM79_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOV86419.1"
                     /translation="MELEKARELTLQLARPAGVEWLWLDDALGRVLALDIKAERSLPS
                     NPRSLFDGYALGGEHTRRDPGIGLVQLDLEPGLLAAGDFREGPLGPGQCIRVLTGASL
                     PGGCDAVIPEEETLPGGDRVEIRGPVSPGFGILPAGSDVREGDTLLTRGCFLTPSRLA
                     MLVAVGVTRAPVFRRPAVAVLATGDEVVELGESAGKSGIFCNNRYLLSWLAGLYGAEV
                     VQLGTSPDDPRIIAARLEEARADVLITTGGTGRGERDCVSRVWQMLGVEPLFRQLNVS
                     PGRNASLGMRDRNVFWGLPGNPWAAQFVFEELIAPMLRRFQGVPNPGPFELPGRTTRR
                     MIKKKGFHKAVRGGLRLQEDVLYFEPLETGAGSTFANIRDHFAYTLLGPDVVEVPAGS
                     SIRIRAHDLPCLPLQWLDSIGLRGRAV"
     gene            20341..20982
                     /locus_tag="PLM79_08660"
     CDS             20341..20982
                     /locus_tag="PLM79_08660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOV86420.1"
                     /translation="MLKGTPVEMGYYRFLERHMVPKLVFLRLTPNHISFLGLITSMLA
                     GLAFAYQPFLGGILTLLTGLLDTLDGSLARSLGQSKKFGAFLDSVLDRYTELIIYLGI
                     WCYFYRGGGATPFFSLIILLILFGSLMVSYTRARAEGLGVRCLVGTFQRGERIILIGL
                     AGILNPCLNWLVGAKTGHWTQDLALVAVLLGLAVGTNLTAMWRILHVLNRLRQ"
     gene            complement(21058..21480)
                     /locus_tag="PLM79_08665"
     CDS             complement(21058..21480)
                     /locus_tag="PLM79_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOV86421.1"
                     /translation="MQVEYKNIDPKLVVNEIRNGRSLESVQKLFGIKFKSEVQDLYLR
                     GLMELGEIPQVDFNKQNSPRFVPSAPDRPVIRRREKPVSKPTMLIKTANFRTIGQSGS
                     ITLNKALLIEQLGFEVGDSFEIYREEDRVVLKKTANPH"
     gene            22469..23740
                     /locus_tag="PLM79_08670"
     CDS             22469..23740
                     /locus_tag="PLM79_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HOV86422.1"
                     /translation="MRGKRWVYPWVLGFVVVLAGAQGVLAAGFALYEGSARGNALGGA
                     LVGRADDPSALYYNPAGITQLPGLQLMAGATIIMPTTDVTIGGIRETTKDNVWIPPHL
                     YATYQFSDRLWFGLAMYSPFGLGTEFDENWLGRYNSYKALIETFSVNPNVAFKLTDKL
                     SLAAGFSVMYFDLTLDRKIPTGQPFWPFPMPWVGDVDADLTGDSFGYGWNVALHYQPC
                     EYAKFGIAYRSQVKQNLSGSAKFTIPAVGENIYRPFLHNTDATGAVTLPDEVYVGLAV
                     YPMKNLSLELGGVWTGWSDYSQLVIAYDRPVAGRKVSVVSKDWHDVWRLNFGIEYKPL
                     NWLDLRFGYVFDEEPIPDRTVDYLVPANDRHLFSFGPGFHWNNWTLDVSYTYLLITDR
                     DVEGRPLDGVFTGKFENGDAHMVGVSLGYKF"
BASE COUNT         4745 a         6920 c         7267 g         4808 t
ORIGIN      
        1 aggtccgtga gggtcagccc tgcgaaatcc gcctggattc catccccgac gagcggttca
       61 cgggcaccgt cttcaccgtt gtgcccacgg cggaccgtac caaggggacg gtcctggtca
      121 aggtccgttt cgacgccctg gacccccgaa tccttccgga catgagtgcc aaggtggcct
      181 ttctcacgcg tccgcttgct tccggggagc tgcaacctcg cctgggaatc caccgggatg
      241 cgttgacgca gcgggacggc atctccgggg tcttcgccgt cgacagggag gtcgtccgtt
      301 gggtccccat ttcctcgccg acgaccttgg gggactttgt cctggtgggc gaccgggtcc
      361 cacaggggac gaaagtggtt ctgaagcctc ccgacgccct caagcaaggt ggaaagatcc
      421 aaattagcga gtcatgattc ctgcgggtgt tatggatttg agttcccacg gggtccggga
      481 cgggagaccc tccggactcg ggagaaccac ccccgcgccc gggaactgac cggagtaccc
      541 gacggggtcc ccggctcgag gctccgtctc gaacccgggc ccgctcctcg gatgaggaag
      601 gacaggaaaa cacggtggaa ccgccccaat ctcccgaacc catggtcatc ctggaaggca
      661 tcggcaagtc ttaccggagg ggcgttcaga agatccccgt gctgacggac atcgacctca
      721 ccatcggcac gggggaaatg acggccctga tgggtcccag cggctcggga aagaccaccc
      781 tgctcaacct cattgccggg atcgaccacc ccgacgaggg agtgctcatc gtagccggga
      841 cccgcctggg agatctgacg gaggtggagc tggcgcggtg gcgtgccgtc catgtgggct
      901 tcatcttcca gttctacaac ctgcttcccg tcctcacggc cctggaaaac gtcgaactcc
      961 ccctgctcct gcgggacctg gaccggaggc agcgccggga acacgccgcc ctggccctgg
     1021 aactggtggg cttgccggac cgtatgaacc attatcccag gcagctctcc ggaggccagc
     1081 aacagagagt tgccatcgcc cgggccatcg tggccgaccc cacgctcctg gtggcggacg
     1141 aacccacggg agacctggac aaacattcgg ccggcgaagt catggaattg ctcgaacagc
     1201 tcaaccggga tttcggcaag accatcctca tcgtcactca cgatcccaag acggcgaaaa
     1261 gggcaagagt gatccaccac ctggacaagg gagtgctggt gaggagcgag aacagccggg
     1321 aatgaaccct cggatcggga cggctttcca tggaattgct gaaacttgtc gtgcgaaaca
     1381 cccaacgtca gcggctgcgc acggccctca ccgtcctggg aatggccgtg gcaatcctgg
     1441 ctttctgcct tttgcgcacg gtggtggagg cctggtatgc cggggtggaa gccgcctccc
     1501 ccaaccgact cgtttgcagg aatgccgtgt ccatcatctt cactctcccc ctaagttatc
     1561 ggcagaagat cctccaggtg gagggggtca cccaagtggc ctatgccaac tggttcggcg
     1621 ggatttacat cgacgagcgg cacttctttc cccgaatggc cgtgggaccg gaggtgtttt
     1681 ttgacctctt tccggaaatc ctgatgcccg aggaacaact caaggcgttt catcgccaga
     1741 ggaacgcctg catcgcgggt cggaagctca tcgaagagta cggctggaag ctgggtgaca
     1801 ccatcaccct cacgggcaac atctatcctg gggaatggcg gttcgtgctc cggggaatct
     1861 accgaggggc caccaagacc gtagacgaga cccagttctt tttccactgg gattacctgg
     1921 atgagagcct caagaagcaa tcgtcgggcc ggagcgggat ggttggatgg tacgcggtcc
     1981 agatacggga cccctcccgg gcgggagcca tctcccaagc catcgacgcc atgttcaaga
     2041 actcctgggc ggaaaccttg acggagacgg aaaaggcctt tcaagtcggc ttcgtggcca
     2101 tgaccgaagc cattgtgacg gccattcgcg tggtttcttt cgtggtgatc ggagtgatcc
     2161 tggtgatttt ggccaatacc atggccatga cggctcgaga gcgtctttgg gaatacgccg
     2221 tgttgaaaac tctgggattt ggaaacgggt ttctctgggt gttgatcgcg ggggaatccg
     2281 tctccatttc cctggtggga ggcctcctgg gaaccctgct gtcctttccc atcgccgggg
     2341 tattcgccca acggctgggg actcttcttc ccattttcca catcagcctg cagaccattc
     2401 ttctgaattt tgtcgtgagc atcgccatcg ggctcatcgc cgccctcctt cccgcctgga
     2461 gagcctgccg ggtaaaaatt gccgaagccc tgacccatgt cggctagatc gttcggacct
     2521 gcggtttccg ccgcctcgta cggagggaaa atcgccatcc tgtggaattt ctttctgaaa
     2581 gtattcgact atgaacccta tccgccgaag ccccgagaag gatccgggaa ttcacctcct
     2641 cgttggattc cgagggcaaa cctttgaaga agaactgaaa gccctggtgc atgaatattg
     2701 cataggcgga attgtgctct tcaagagaaa catcgaagct cccgaacagc ttcgttgcct
     2761 cttggaagat gcccaggcat tcgcccggga atccctggga cggcgacttt gggtggccgt
     2821 ggaccaggag ggaggcccgg tccagaggct gggtcccggc tttttccagc ttccctccgc
     2881 tcatgaactc ggcgtcgcag gcgcccgttc cgtgaaggag tgggggaaga aggccgccat
     2941 ggacctgcgg agaatgggca ttcagatcaa cctggcaccg gtcctggacc tggtgggccg
     3001 ggattcgacc cattttatgg aagcccgctc cttgggctgg gactccgagc aggtggccga
     3061 cctgggaaag ctttggattc gggtccttca ggaaaacggg gtgtcggcca cggccaagca
     3121 ctttcccggc ctggggcggg cggaatccga ccctcatcat tatgctccgg tcatccactg
     3181 ggccgatgaa gaagacatga accgagacct ccatcctttt cgcgaagcca tccgtgccgg
     3241 tgtccactgc gtgatgactt ctcacgccct ttatcctctc ctcgatcccc ggtggcccgc
     3301 caccttgtcg ccccaaatca acggggaatg gttgagaggg aagctgggat tccagggggt
     3361 ccttttatcc gacgatatgg acatggcggc cgtctcggag aggtattccg tggaggagat
     3421 tgccaggcag gggtttgcct ccactctgga ctttttcctg tcctgtcagg tatcggaaaa
     3481 catcgagccc ctggcccgcg ccctggccga cggttgccgc cgggaccccg tgatggccca
     3541 ggcccatcaa agctctttgg agaggctcga aaggttgaaa aaacgccatg atggctaatg
     3601 gaagcccgcc cggcattccg ccgggaggag caagggaacg ggtatccgga cggcgccgca
     3661 cggcggccgt gttcctggcg gcgatcctca ccgtcctggg gatatcggcc gggaactcgt
     3721 ccggtcaatc tcggcaagtc ctcattctcc attcctacca tagcgggctc acctggaccg
     3781 attcggtcat ggacgggatc aagaggggtt tttccgaaag caaaatcgac gttcagctca
     3841 gcgccgaata cctggacgcc cggcgatacc cggacccggc tagcagcaaa ccctttcggg
     3901 acctcatttt gctcaagttg cagaccctca ccccggacct gatcctggtt tccgacaaca
     3961 gcgctctcgc gttcctgctc gagttccggc agcggctttt tccccaggtt cctgtcgtat
     4021 tctgcggcat caacgatttt catccttcca tgatcatgga gcaccggcaa atcaccggcg
     4081 tggccgagga gatttcggtt ctcgagaccg ttcagctggc catgcgactc cagcccgaaa
     4141 cccgggagat cattgtcatc ggccgcacct cggtgcccgc cgacaaggcc aatcgggaca
     4201 gtttcaccgc cgccctctcc gatcttccca gccagctcaa ggtccgcttt tgggacgact
     4261 tccccctcca ggagctgaaa accaaactcg aggaactcag accggggtcc gtcgtcttca
     4321 tcaacggcct gctccaggat gaaaccggac accagctcat gtacggggaa accacccggt
     4381 ggatctcccg gcacagtccc gttcccgcct attctttttg ggatgtctac ctgggtcacg
     4441 gcatcgtcgg ggggaagctg gtgagcgggt accaccaagg gaaaacggcc gcccaattgg
     4501 gtttacggat actgaagggg gaaagtgccg atcacatccc ggtcatcacg ggagggcagg
     4561 caaaccttta catgttcgac taccggcagc tggagaggtt ccatatcccc tcctccgccc
     4621 ttccggacaa tgccaaggtc ctccatctcc cggaagcctt ttatcaccgg cacaaaaagc
     4681 tggtgtgggt caccggagcg atcatcgcga ccctgagcct ctttgtggtg ctgctggcct
     4741 cgagcattct tcgtcgacga cgggcggaag cggccttgcg agaaagcgag gagaagtatc
     4801 gcgagctggt tcagaacgcc aacagcatca ttcttcgcat ggatccccag gggaaaatca
     4861 ccttcttcaa tgaatatgca gaaagttttt tcggataccg ccaggaggag atcctgggca
     4921 agagcgtcct ggagaccatc ctgcccgaaa aggaacacgg cggccgggac cttcgcgccg
     4981 tgctccggga taccatcact cacccggaag accatccggt gcaggaacac caaaacattc
     5041 gccgaaacgg cgagagagtc tggatcgcat ggggcaacaa acccatccgg gacgaccggg
     5101 gggatctcgt ggagatcctt tgcgttggaa atgacgtgac cgagcgcaga caagccgaag
     5161 aggccctgcg ggaaagcgaa ctgcgctggc agttcgccct ggaaggggcc ggagacggtc
     5221 tttgggactg gaatactcgg accgacgagg tgtacttctc accccagtgg aaagcaatgc
     5281 tgggatacgg ggaggaggaa atcccccacc ggctctccga atgggaccgc agggtacacc
     5341 ccgaagacaa ggaatacgtc taccgggaaa tcgagaggca cttttcgggc cagactcccc
     5401 tctacaccag tgagcatcgt ctcctttgca aggacgggac gtacaaatgg atcctggatc
     5461 gggggaaagt aatccgccgg gatgaagacg ggaagcccct gcgcatgatc ggcactcaca
     5521 ccgacatgac ggagcggaga cgaggcgagt ccgaccggat cgagatggaa cggcagcttc
     5581 tctacgccca gaagctcgag agcctggggg tcatggccgg aggaattgcc cacgatttca
     5641 acaacctgct catggtgatc ctgggaaacc tggacctggc tttgcatgaa atcccctccc
     5701 agtcctccgc agcccaaagc atcgaacagg ccatccttgc gtcccgtcga gcggcggatc
     5761 tcacccttca gatgctcgcc tactcgggca agggccgcta ttttctcgaa gacttcagcg
     5821 tcggggagtt gatccgggaa aacgcccacc ttcttcgggc atccatctcc aagcactcgg
     5881 ctctcgtggt tcaaacggag gcaaacctgc cgcacattcg cgcggaccgc ggtcaggttc
     5941 aacaggtgct catgaacctc atcaccaacg catccgaggc catgggagag aagggaggag
     6001 ccatccgggt tcatgcgggg gtcggggatt ttgatcgggc ggagcttgcc cggagcaaaa
     6061 ccgacgagaa gcccccgccg ggaccctatg tcttcgtaga agtcagggac acgggatgcg
     6121 gcatgaacga ggaaaccctg aggcgtttgt tcgagccctt cttcacgacg aaattcctgg
     6181 gcagaggcct ggggatggcg gcggttctgg ggatcgtccg cgctcaccac ggggccatct
     6241 tcgtggattc caaaaacggg gaaggaacga acgttacggt attgtttccc gtatccgggg
     6301 aggctcggcc ggccgccgaa gaaattgagg aaagacaaaa agaggtctcc ccggctgttg
     6361 aaccctcggg ggacgtttcg acccgggcga tcttggtggt ggatgatgaa gacctggtgc
     6421 tcgaagtgtg cggcaccatg ctggagagcc tgggctatcg gatcctgacc gccacgaacg
     6481 gagaggaagg ggtgaaggtg ttcgaggaac acgccgggga catcctgtgc gtcttcctgg
     6541 atctcaccat gcctcgcatg gacggggttc gagccttcca cgaaatgcgt cgcattcggc
     6601 cggacgtgac ggtcatcctt gtcagcggat acagccagga cgaagccacc caacgatttg
     6661 ccggagaagg gctcgcggct tttctccaaa agcccttcgg aattcaaaag ctggcaacga
     6721 ccctcaagga agccctgggg cacgaacggc cctaagttgg gggcctggag gccgggggac
     6781 ggggcccggc cgaagccttt ctgcgtcgat cccgcttaca gccgtgacca ctgcattcac
     6841 aaccaggtgc gatggagtgc ggcgaccctt tgcggaagcc ggttggcttc ggaccgagga
     6901 tgcccgggag acgaatcggt ggcgccgtat tgcagggcgg tgcaagaggc tcctcaccgg
     6961 acacccgtgt ttgaggtcgg gatcggaagt tcggggcagg ctcgattcca ggtccgtgga
     7021 tttgagggca caggcggagg tttccgcgac gccgcaggaa gcggcgtcgc cctggtccgc
     7081 ctgttccccc gtgtgggttc aaccccggtc tgccgggaat gatgcccggc ggtcgcacag
     7141 agccccctca ggcgtccaag tcccggagca ggtcgtggag gcaatcttcg gcccggtatc
     7201 cttggagtcg ggccagattg acgagggaca gcagcaattg ggcaaagtcc cggcccgaag
     7261 ggtcccctcc cccgtggatc ctcgaggcca ggtcttccat gcggtttcgc aggtcttcga
     7321 aaagtcgcct cggatcgttc cagggggatc cctcccgatg ggaaagacgg gagaccgtgc
     7381 ggtaagcccg catcagggcg ggcagcgttt cgggaaccgc cgaggggttt ttcccccggc
     7441 cgcttttttc atcggccttg atcttttccc agttgtcctt gacctcttcg gcggtctttg
     7501 ccgtgacgtc tccgaagacg tggggatgcc gccgtatcat cttttccgtg atccggtcgc
     7561 acacctcttc cagggtgaaa tgacgatctt cctcgtagag gtgcaccagg aacagggtca
     7621 tgaagagcag gtctccgagc tcttcggccg cttcggaggc ggacccggtg cgaaccgccg
     7681 ccgccgcttc atgggcttct tcgatgagat aggcctgaac gctcgccggg gtttgtttcc
     7741 ggtcccacgg gcagccgccc tcccctctca gccggtcgat gatctcccag attcctcgta
     7801 gtttgtccca acgtgaacga tgatcacaat tcctcatggg gcgacttctc ttttcctctc
     7861 tttccggggg acggctttga cgcctcccgg gattggtccg accaatactt ttcgatttcc
     7921 cttcgcttcc ggtggaattg atcctggagg cgttggggcg tcgccatgac caggatacgg
     7981 gcgaactggt ggacgtaggg cagcagggtc gatcggcgca aatgcgggct ctccgagggc
     8041 agaaaaaggg tcaggagcca caccaggatt acggcaagga ccccggcctt ggcgaaaccc
     8101 acggtgccgc ccaggaggcg gtccaggaaa cccaaacccg tcaggcgaag cagtttcccc
     8161 aggaagtatc ccagcattcc cacgcacaac caggtgagga agaaaatcag cagaaagctg
     8221 agaaccgggg cgaaccccac ctttgggagg gtgtcggcga tctgacgggc cagggcttca
     8281 tgattgcgcg aagccagcaa aaatcccccc agaacgcccc caatcccaaa gacctgagaa
     8341 acggcccccc tccaaattcc tcgaaggatg cagatccctc cgaccactac gatgacccag
     8401 tccagtccgt tcattcgttc tccagatcct gccgggacaa tgacttgtgg gttcggattt
     8461 cgccccgcat gcagtataat ccgccgttgt ccgggatagg atcatacatt ttttctcgga
     8521 aaaacaccac gccgccgacc ctgtcgagga agggccaagc gttttccccg ccacggagag
     8581 aggcgaagac ggcggttgcg gggtgcacgg tcggggtttc caggaagcag gaagagcgag
     8641 gggccgggag acgacggctt ccgggaaagg ggcgcacgga tgtaccggga aattcgcaaa
     8701 ggtcgcacga tcatgggacg gctcgccaag ggggtggact tattggctgc cctggaacag
     8761 gtgtgccggg aaacgggggt gaacctgggg gaactgacgg ccatcggagc cgtcagtaag
     8821 gctcgggtgg gctattatga ccaagaacgg cgcgtatacg ccttcgaaca atgggatcgt
     8881 ccgctggaga tcctctcact tgccggaaac gtttccctca aggacggggg gccttttgtc
     8941 catgcccacg tcaccctggc cgacgatcgg ggaagaggat tcggagggca tctggcggaa
     9001 ggcaccgcgg tatttgcctg tgaattcgtt ctccgggaat acctgtcgga gaaagaatac
     9061 gtgcgggccc cggacgcgga cacgggtctt tccctctgga aggatgcggg ataagccttc
     9121 cggcgcatcc cccggacctg aaagcccggg ggttcgccct cgggagccgt gacctcgatc
     9181 tttgatccac cggcggggcc cggacgcagc cgatgaatcg cccgggccgg aggacccggg
     9241 tgtccgccgc tccgctcaag cggtttgcgt caggagttct ctccctgctc ctcccttttc
     9301 cgctcgatat accgggagaa ttcctccatg acatccgcct ggcctccgca gttgtaagaa
     9361 acgacgtttt tcaggtggat gaaggaatcc agatccatct tccggaattg gacggccact
     9421 ccttcttccg cttgtcgcac cacaattccc tgcaatgaga tgctcaactc cgaagaactg
     9481 cccgacagga ggatctccaa ctcgacgggc tcgttcacgg agatcggctc cggagttttc
     9541 agaagcaggc cgttcaagct gaggttttcc aaatcgcaca caagggtctt ctgggaacac
     9601 ttcacgattg cctcggattg aaagggaatc cgcgtgaagc tccgcttttc ctcaccggct
     9661 ggttccataa atcgatccac tcctctgaaa aagggttcgc tcaaaccgga ccgttcgtgg
     9721 gcactttgcc cgataggggg tagaaactgc aatcctggga aatgggagac ccgtttcttg
     9781 tttcccgtca ccctaatccg gaatccgtgg aatgacaact cgaaaattcc cgttcgaaat
     9841 ccatggggtt gcggcatgcc gagggccgcc gaacgatact cccgtgtgct tctatccgga
     9901 acccgggctc accccttctc ccgggttcca cccaaaacaa gacccggcga cggcgttgag
     9961 tccatgcaag ttgactaatg acggaaacga gaatagaagg aagtaacctc aacccccatt
    10021 tggatgaata cattctatga aacggtcatc tgacagagac gatccggtcc aaaagatctc
    10081 gattaacgga ttgagcgaga gggaattcct ctctgtatac aatgcgggca aagttcttaa
    10141 gctgaacgtc ggcgacaacc tcgtgaagga gggtacgccg ggcgacctgc tcttcctggt
    10201 tctcaagggc aacttggccg tgtgcgagga attccccggc gactccggcg atgtcgtgtt
    10261 tcgggaaggc gactggatag gggaaggagc ggttccggag ccgctcgatg gaaggggttt
    10321 cctgacggcc cgaaaaccgt ctttggtatt ggccctggat ccctcggccc tggaaaacct
    10381 ggaccctggg gtgcggaact ttgtctggag aaagctccac aaggggttgt tggcgaggat
    10441 tcgttccttg gaaaagagtt cgagggatct gttttcctcc agccgtcggc aagggatgca
    10501 gatgcgggag ctcctccgga aaaggacccg gggctacgag cagtcggaaa tcatcctgag
    10561 catcctcaag aacgtgcccc gtttgcccat gcacgtatcc aagctggtgc aggtgttgtc
    10621 ccaggagggc acttcggcac gggaagtgac ccaactggcc aaggaggatc cctccctggt
    10681 gggcgagatc ctcaaaacgg tcaactcttc ctattacggt cttcagaaaa ggatctccga
    10741 cctttcctac gccattgtct atctggggtt caaccaggtc taccagatcg tcatcgccaa
    10801 cggcttgcgc aaagcgatgc cccgcacaca caggttcgag gagctctacg aacactcggt
    10861 ggtcatcgcc cacctggctt tcgaaatcgg gcagctctac gaccggcgga agtcttccac
    10921 catgggcacc attgccctcc tgcatgaaat cggagagagc gtggtgctgc tgctcaaggc
    10981 ccagaatccc cgatggtcct ttttcatcga catgctggag ccttccaagg tcgctgccat
    11041 gctgctcaag gattggaaca ttcccgacaa tatctgtgag acggtggaat accagtacta
    11101 tccggagttt gtacctccgg gggacatacc caccccctac aaagactgtg tcgccgtgct
    11161 gtacctggct cacgccgcat gcgactgcat cgggggcggt gccgaaggca tcctcaggca
    11221 gccttacctc aaggattacc ttcaatttct cgacatgccc gaggcaaccg tcagcggtct
    11281 cctggacaaa tatgtgctca cggacttgaa catgaagatc cataccttac ccaagcacgt
    11341 acagtcattc attctaaaaa ggaagggggg gtgagcgccg tatcgagatc gagggatgat
    11401 tcccggcgcc tttgacgcgg cccggtcgtg agccccggct tgagcgcaac ggggggacgg
    11461 gaatggagga gcaccagggt aagcaataat ccacggacac caggaaggag gtcactcatg
    11521 aattggatgg gtttgttggt tgcgctactg atctcggtca tgacgctcca ggtcgctccc
    11581 gtctacgcgg agggaatgca ggacgatgtc gatcaggcgg ttacgattat caagcgtttt
    11641 caagatattc cggaaaaatc cattcccagg acggtgcttc gggatgcccg gggtattgcc
    11701 gtccttaccg tgatcaaagc cgggttcatt gtgagcggca ggggcggaac cggggtggtg
    11761 gtggcccgga ccaagaaggg atggagcgga ccttcggcca tcgggaccgg cggggcggga
    11821 ttcggcctcc agattggagc ccaggtttcc gaattcgtgt ttatcctcaa taccgatgag
    11881 gccgtgcggg ccttctcccg ggaggggaac gtgaccctcg ggacggacct cagtgtggcg
    11941 gccgggccta tcggccgaag cctgggagtg ggggtgactc ccgtggcggc tgtctacacc
    12001 tacagccgaa gccaggggct ttttgccggg gtttctctcg agggaacggt gatcgggacc
    12061 agaaacgatg ccaacaccaa gtactacgga aagcccgtca gcccccacga catcctgacg
    12121 gggagggtga aagcgcccgc cggagccagg gcactccaga aaacccttgc caagtactaa
    12181 ccgggtttcg ggtcggcagt tccaaagaag aaccgtcacg gaccgggttc cggctccgtg
    12241 acggtttttt cggcccctca gaccgcctcg atggggtcgt atccgtcaca aatggggtcc
    12301 aggatcttcc gggattcctc ggggaggtcc aggaactgca cgccgaatcc cggagggtag
    12361 tagaccactt gcccccgatt cacccaggtc actcttcccc ggcaggaaag aggagaatcc
    12421 tttcccagcc agagttggag atgaatctgg ctcgaaggcg gcaccttttg actcgtgatg
    12481 acaaagattc ctcccgggca gatgtccaaa gtcaccccgt cgtgaaccac atcttcgttt
    12541 ttccattgaa tccgtatgac ttttcgggtc cgcttgcttg atcgccgatc catgccctct
    12601 ccctgcgcgg aatctccaac caatgcgacc gccttcccaa gacgttcccc gccgtggggt
    12661 ccccttcaat gggatgaaat tataccctcg actctccggg acccaaagag aaaagacccg
    12721 cccgcatcgg gccgtcggcc gccggtttgg agggattggg ttgaaatgtg gaagggtcct
    12781 tggggacggt cttgtcctcc caggctcctg gtcccgcatc ccgccggtgc caaaggatcc
    12841 aaccgacggc ccgacggccg ggagtccgcc ccacgactcg gacccgctag gagggagccg
    12901 gcccggggcg ggaggagggc tcgggaccct tggagaccct cacatacacc gcgtcccgtc
    12961 cccgtcgctg gatggacccg ctcccgccgt tgtggcggat taccttgagc attcgatcca
    13021 gttgttcatg ggcgcagatg attccaaaac tgtttccttc gcgcagaaat ctgagacgca
    13081 aacttacgtc caggtatccc gctcagcagt ctccccggta gtccgcccac tccgagtgat
    13141 tctctccctc gttcagtcgg tcaggcatac ctccgcttcc ctggtgaggt ccacgcgaat
    13201 ttcgtacact ccccgggaga aggaagtctc catgaggtac cccgatcttt gaaacactcc
    13261 cagtaccttc tcgtttccca gagtactgta ggccaccaag gtgcggatct tcttacggcg
    13321 ggcgatgcgc atcaggtggt ggacaaggaa actggcgatc ccttttttcc cgaagtccgg
    13381 atggaccgca aagtcaatct ccccctcgag gttttcttcc ttcaacaggt atcgggcaat
    13441 ggcgatgatt ctctcggcat ccacctcgcc caccagtccg aagagggtca attcgcggtc
    13501 ataatcgata ttgaccatgc gttgaacgtc gtaatgaggg tacaccctca tgttcgacag
    13561 aaaccggacg taggcttcct ccctgggcaa agagtagaag aattccttga gcgcccgttc
    13621 atcggtgggt ttggcaggcc tgaagaagac ggtcaggtcc gtatcgaaga gttgcgaggt
    13681 ttcgtgctcg gccgggtaaa cggccttggg ggcgattacc ggaaaggcct cctgccgcag
    13741 gtagttgaat tcgtaggccg cccgcatcaa ttcttcccga aagtcggggt gtgcaatccc
    13801 gatgagagcc agggcccgct cccggatgct tttgccgtgc aactgggcaa tgccgtattc
    13861 ggtcaccacg tattgaacgc cgccccgggt ggtcacgacc ccgccccctt cggtcaaact
    13921 ccggacgatc cgggacctct ggccgtccaa ggtcgtcgag gggagaacca cgatggcttt
    13981 tccccggtac gaatgccggg cgcctcgaag aaaatcgatg gttcctccca ctccgctgta
    14041 aatctcatac cccacggaat cggcgcatac ctgccccgta aggtccactt ccagggcgga
    14101 attgatggaa accatcttgt cgttttccga gatcaccatg taattatttg tgtaatcaat
    14161 gggatacatt gccaccatgg gattgttgtg gacaaaatcg tagagctccc gggaccccaa
    14221 acagaagctg gcgacaatct tacccggatg aagggtcttg cgggcattgg taatgacccc
    14281 cttcttggcc aggtacatgt aggtgtccgt gagcatgtcg gcatggacgc ccaggtcctt
    14341 cttgtcgtga agggcataga gaacggcggt ggaaatgctt ccgaccccca ctcggatggt
    14401 ggagccgtct tcgatgagct tggccacatg cctgccgata tcttcggcga tcgggttcat
    14461 cagcggctgg cctacctcga gaaggggctc ttcgtgctcg acgatggcat gaaggtcccg
    14521 caggtggatg aaggaatctc ccagggtcct gggcatctgg gggttgacct gggcgatgat
    14581 gaacttggcg ttttctgcgg cggccttgac aatgtccacg gcaatgccgt aactgcagaa
    14641 cccgtgctgg tccggagggg aaacctgaat gagggcgatg tccacccgga cggccccggt
    14701 gcggaaaagg tcgggaatgt ctcccaggtt gatgggagta taatcggcgc gcccttcggc
    14761 aacggcctcc cggcttgccg cagccacaaa gaaggatttt agccggcatt tgtcaaagaa
    14821 gctcgcttcg gtgtacccgc ttcgtcccac actgagaatg tgcaggattt ccacatcggc
    14881 caggagcggt acgatttcct cgagggcgcg gacgagatgc tgaggctctc cgcacccgga
    14941 accgatgaaa acccggttgc cgcgcttgat ttttcgcagg gccttggccg cggtggtttt
    15001 ctttgcctgg tacatggact gccagggccg agcgttggaa ccttctcggt caaaaaccgt
    15061 tggttgggcg gaaatgtctg ccatgggctc gtcctcgtat gggactcaag atctcagcct
    15121 tgtcccgtct gtggggtccg gtagctcaaa aaggtggcca gaagggcgcc ggcggcaatc
    15181 atgctgaggc acagcacctg ccccatggtg aaaagacccg cgaggaagcc gatctggggg
    15241 tccggctccc ggaagaactc gaggaagaac cggaaggccc cgtagaacac gaggaagaag
    15301 accaccatca ttccgtcccg gaagtccttc ttcctgagga cccaaaggag caggaaaagc
    15361 acgagtcctt ccagaaacgc ttcatagagc tgggaaggat ggcgcggaac gggtccgcct
    15421 cccgggaaaa ccatggccca gggcacttgg gtgggacgcc ccaggagctc tccgttgatg
    15481 aagtttccga ttcgccccag tcccaatccg acgggaacgg tgacggcggc actgtcggca
    15541 atcacccaaa agggaaggtt tcttcgcctg caaaagatca agccggcaag aacggccccc
    15601 acaagccccc cgtgaaaaga catgcctccc cgccaggtgg cgatgatttc cagggggttt
    15661 tccaggtaga aaaggtatga gtggtattga tagaatacca ggtagcccaa ccgagcgccc
    15721 acaatgaggc cgatggccag gtaaaacacg aggtcctggg caacgactcc cacgaggccg
    15781 acctggcggg atcgttcctg cctttgaatg aaaaaatagg ccgcgacaaa gcccagaacg
    15841 tacatgacgc cgtaccatcg aatatgaatc gggccgatgg tgacgaggtc cggatcaatt
    15901 tgcggatacg gaatcatcgt ggactgcacc tctttccaac cgggaatgta cgcggataac
    15961 atagcactcc gggggcggca attcaaccgg agaggccctc ctcgggaatg atggcgggag
    16021 agcggccatc gggatcgtct ccggttgggg aacggggcgg gaaggtcttt tttccagggg
    16081 accgccgtcg gcacggagcg gcccccgact tcgaggggcc gtcggacggg accgcggacc
    16141 tcgggagcca aaaagccggc tgcaccgcgt tgacagccgg ccgaaacctg tgacatagta
    16201 agtatttggc aagggacatg ccgcatgggt ggagagagat cttggagtct tctgcaacgg
    16261 aaccgtttgg atgtctcatc catggtcgat tggaaatggg agcggagacc taccccgtct
    16321 tcaacccttc aacgggtgaa gtctttgccc gggtcaccac cgccgatcga tccatggttc
    16381 accgggccct ggagagtgcc cgggagagtc gtgggtcctg gcaacgcctc ccgtcgtccg
    16441 aacgggcgga ccttctccac aggctggccg caaaggtccg agaacaagcg gacccgttga
    16501 ctttcctcct gtgcaacgaa gtgggaaagc ctactcagag cgcccgggaa gaggtgcacc
    16561 acacggccgc cctcctggat ttctttgccg aggagagcct tcggcttctg ggacggatac
    16621 ccctgcgcgg gtaccccggg gaacaggtcc tcattgtgag gatgcccgtg ggagtggtgg
    16681 tcgccatcac gccgttcaat tatccgttga gcaccctggc atgcaagatg gctccggcct
    16741 tgggagtcgg ttgtaccgtg gtggccaagc cggatgaaca tgctcccctg agcaccctcg
    16801 agatggcaaa gctggctttg gaggtgggct ttcccccggg agtgctcaat gtcgtcacgg
    16861 gcccggggcc cgaggtggga cctctcttgg tggaccaccc catcccgggg ttgattgcgt
    16921 tcacggggag cctcgaagtc ggcaggcagg tgcaggccat cagcgcccgg tgggttcgca
    16981 aggtagtgct cgaactggga ggccattgcc cggcctttgt ctgccatgac gccgactggc
    17041 ccgctcttct ggattcgctg gtattgcaga gcctgaagaa cagcggccaa tactgctacc
    17101 gtcccagcca gatctttgtg gacggcagga tctacgagga tttcgagaag cgattcattg
    17161 cccgggcggc ccaatgtcga gtcggggccg catgggaacc cgacgtggac ctgggccctc
    17221 tgaacaaccg ggagctcctg gccaaggtcg acgaccaggt tcggggggcc gtgcaggaag
    17281 gggcacacct ggccctgggg ggctatgtcc cggagcgtcc cggattcttc tatgccccca
    17341 ccgtcctgac gtgcgtgaaa ccccagacgg ccatcatgca ggaagatgtg ttcggaccgg
    17401 tggtcatgct ccaccgagcc gaggacatgg aggctgccgt cgaccaggcc aacgcgtctc
    17461 cccacggtct ggcggcttat cttttcaccc gggatctggg atctgccatg gaatgggcaa
    17521 gccgcatcga ggcgggcagc gtttgggtca atcgaatcca tcaggcatat cccgaggccc
    17581 ctttcggggg gatgaagcaa agcggcttgg gacgggaaaa gggtcatgcg ggcgtggaag
    17641 aattcacgga gttgaaaacc atttacctgt cttacggaag tccacccctc aacaaatagg
    17701 aaggccgaac gccatgttgc ccattgtgtg tgttgtaggt gcgtccaacg tggggaagac
    17761 cacgttcctg gaaaagctca tcccggaatt gacgaagagg gggtaccggg tgggatgcct
    17821 caaacacgat gctcacagtt tcgagatgga ccacccggga aaagacacgt atcgccaccg
    17881 gcaggcaggg gcccggacca ttgccatcgc atcccccgat caggtcgcgg ccatccgccg
    17941 ggtggactcg gaaccttccc cggaagaaat ggcggcacgg ctcttctggg acgaggacat
    18001 ccttttgacg gaaggctaca aacgttccaa tttacccaaa atcgaggtct ttcgctcggc
    18061 aatcgagtcc aaacccatct gcacccggga ggaccacctg gtggccctgg tgaccgatga
    18121 tgccgtggac gtccaggtcc cgcgattcag gtttcatgaa gcggacaagg tggcggattt
    18181 catcgaggaa cgatttctga aacatcgcaa aaagcacggg gtcctcgtgc agctcgacgg
    18241 aaaacagctc cccatgaagg aattcgtgcg ggatttcgtg gtgggagcca tccgaggaat
    18301 gctctcgagc ctgcggggct gggaagatcc ccgaacggtg cacatccaca tccggttgaa
    18361 ggaccgttag gtggtcacgg gggccattct ggccgggggc agaagtcggc ggtacgggag
    18421 gaacaaggcg ctggaggtct ttgacggcct gcggctcatt gatcggggaa cggcttcctt
    18481 ggaaccctat tgtgcccccg tgttcgtggt tgccaacgat ctttgggaat acgcggatgt
    18541 gcaggcggtc ctcctgcggg acctcattgc ccaccagggc cccctgggag gcatcgccac
    18601 ggctttgtgg ttcagtcccc acgaatgggt gttcgtcaag gccgcggata tgcccttcct
    18661 ggttcccgag ctcctgaccc tgatgttggg agcaaaggaa ggcttcgatg ccgtggttcc
    18721 cgtacacagg gaactctacg agccgctgct ggctctctac catcgcagat gtcttccggc
    18781 agtcaaccgg gtcctggaag gacccgaaag gaaggtcatc gccttttacc ggcaggtaaa
    18841 ggttcgcttt ctccaggagt tcgagtggaa aaccgtggat cccaaggccc tcagcttcat
    18901 gaatgccaac acccccgaag aaatggcggc cctggaatgg aactggaaaa agcacgggag
    18961 ctgacgctcc aactggctcg gcctgcggga gtggagtggc tctggctgga tgacgccctg
    19021 ggcagggtcc tggccctgga catcaaggcg gaacgcagcc tgccctcgaa tccacgctcc
    19081 ctttttgacg ggtatgcctt gggcggcgaa cacacccgga gggacccggg aatcggtctc
    19141 gtccaactgg acctcgagcc cgggctcctg gccgcagggg acttccgtga gggacccctg
    19201 ggcccaggac aatgtatccg ggtccttacg ggcgcatccc ttcccggcgg ctgcgacgcc
    19261 gtcattcccg aagaagaaac cctcccgggg ggggatcgag tggagatccg ggggccggtg
    19321 tctcccggtt tcggaatcct gcctgcaggc agcgacgttc gggaagggga cacgctgctc
    19381 acccggggtt gtttccttac cccgtcgcgg ctggccatgc tcgtggccgt gggcgtcacc
    19441 cgggcacccg tgtttcggcg ccccgcggtg gcggttttgg ccacggggga tgaagtcgtc
    19501 gagttggggg agtcggcggg aaagtccggg attttctgca acaatcgata cctgttgagc
    19561 tggttggcag gcttgtacgg ggccgaggtg gttcagctgg ggacctcccc cgacgacccc
    19621 cggatcattg ccgcccggtt ggaagaggcc cgagccgacg tgttgatcac cacgggggga
    19681 acgggtcgag gggaaagaga ttgcgtatcg agggtctggc agatgttggg ggtggagcct
    19741 ctcttccgac agctgaacgt atcgccggga aggaacgcat ccctgggaat gcgggatcgg
    19801 aacgtgttct ggggcctgcc cggaaatccg tgggcggccc aatttgtctt cgaggagttg
    19861 atcgcaccca tgctcaggcg gtttcagggg gtcccgaatc cgggcccttt cgaattgccc
    19921 ggccgcacga cccgcaggat gatcaagaag aaagggttcc acaaggcggt caggggaggg
    19981 ttgcgcctgc aggaggacgt cctttatttc gagcccttgg agaccggagc aggctcaacc
    20041 tttgccaata tccgagatca ctttgcctat acgcttttgg gacctgatgt ggtagaagta
    20101 ccggcaggga gcagtatccg gatacgcgcc cacgatctcc cctgcctgcc ccttcaatgg
    20161 ctcgattcga ttgggttgcg gggaagagcc gtctaaggcg gtttgggaaa cggggttttc
    20221 cggactcggg aagaaaagag ggagggagat tccttgtcaa ccggcattcc gcccagcgaa
    20281 atcccttttt tcgcatccgg atcccagacc gtctgaagtg actttcatcg agaggatcca
    20341 atgctcaagg gcacgcccgt ggaaatgggc tactatcgtt tcttggaacg acacatggtt
    20401 cccaaactgg tcttcttgag gctcacgccc aaccacatca gctttctcgg cctgatcacc
    20461 agcatgctgg cgggccttgc ctttgcctat cagcccttcc tggggggcat ccttaccctc
    20521 cttaccggac tcctggacac tctggacggt tccctggctc gatccctggg gcagtcgaaa
    20581 aagttcgggg cctttctgga ttccgttctg gatcgctaca cggagttgat catctacctg
    20641 ggaatatggt gttatttcta ccgcggggga ggagccacgc cgttcttttc cctcatcatc
    20701 ctcctgattc tgttcgggtc cctaatggtg agctacaccc gcgcccgagc ggaagggctg
    20761 ggggttcgct gtctggtggg aaccttccag aggggcgaga gaatcatcct cattggtttg
    20821 gccggtattt tgaacccttg cctcaactgg ctggtggggg ccaagaccgg tcattggacc
    20881 caagacctgg cactggtggc ggttctcctg ggactggccg tgggcaccaa tttgacggcg
    20941 atgtggcgaa tcctgcatgt gttgaatcgg ctgcgtcagt gagggcgatc tttgcccggg
    21001 acgcgagggt ctcggcggtg tatactcgag ggttttccgt ccgtcggagc cgtggttcta
    21061 gtgaggattg gcggtctttt tcaaaacgac ccgatcttcc tcccggtaga tttcaaagga
    21121 atccccgact tcaaaaccca gttgttcaat gagcaacgcc ttgttcaggg tgatgctgcc
    21181 gctttgccct atggttctga agttggcggt cttgatgagc atggtgggct tggagaccgg
    21241 tttttcgcgg cggcgaatca cgggccggtc cggggcgctc ggcacaaatc tcggggaatt
    21301 ctgcttattg aagtccactt gtggaatctc gcccagttcc atgaggcctc tcaagtaaag
    21361 gtcctgaacc tcgctcttga acttgattcc aaaaagcttc tggaccgatt ccaaagacct
    21421 cccgttgcgt atttcgttca caacgagctt gggatcaatg ttcttgtact caacctgcat
    21481 tccttgttcc tttctttaaa cgataaacct gcctgccaaa ccggtcgtca aagtgcgcgc
    21541 cctaatgtca agggtgaacc gacccccgac tttcgcagcc tgggggatag ttcggcttgc
    21601 gcggtcccat ccgttgtcgg gagttcccgg cttggttgcc cgggtacgga agaccttccc
    21661 tcacaacata atccgatccc gggaggaacc tttcacgggc tcgcaggtgc gtcacctccc
    21721 ttgagcgatc tcccagaacg aatgccttct ttcccacaaa ccttcttggg tggcggagga
    21781 atctcgagac tgacctcttt cttcgctctt tctacttgaa gacgctggct acaggaaacc
    21841 tgccgctgtc ctgtaattgg gattcaccac ttggcgaagg atttatgatt gtgcggaatc
    21901 gccctccggt gcggagagct caaagtcagt catagcgtaa tgaatgatcg gtcccttgtc
    21961 aaggagcgat ccgtattctc ttttgatttt cctgatttcc gtcccaagcc ggacccgtgg
    22021 tgcagggacc cggttcgatt cgaccccggg gacatcctga ccgtcaagga ctcgtcgcgg
    22081 actcccggat ggttattttc caggaatcgt gtttttcctt gacggaaacg ggaatgctct
    22141 gttaccttgg cgctctcttt ttttcaatca taagtgcaac ttaaccatcg gttcctcatg
    22201 cggcgcagcc tgcgtcgcag gtcgtacggg caggacgccc cggcaccctc ggattctcga
    22261 tggtggtggg gtcttctgcc gcaccgaatg tctcgagagg catccctgga cgtctccgtt
    22321 gggtttatcg tcgtgttccg ttgtaggaat tttttagggt tgtgtccggc cagggaaact
    22381 tctgtcgggt tttcacatat gcggtatggg gcgatcgccg gcgtgggttc atgggttgaa
    22441 gcggaccggg aagcagagga ggaaaaggat gaggggaaag agatgggttt atccgtgggt
    22501 tttggggttc gtggtcgttt tggcgggagc gcaaggggtc ctggcagcgg gctttgctct
    22561 ctatgaggga agcgcccggg ggaacgccct gggcggagca ctggtggggc gggcggacga
    22621 tccgtccgcc ctttactaca atccggcagg aatcacgcag ttgcccggcc tgcagctcat
    22681 ggcgggcgcc acgatcatta tgccgaccac cgatgtgacc atcggaggga ttcgagagac
    22741 caccaaggac aatgtttgga ttccgccgca cttgtacgca acgtaccagt tttccgaccg
    22801 attgtggttc gggctggcca tgtattctcc gttcggactg gggacggagt tcgacgagaa
    22861 ctggctgggc cggtataatt cctacaaagc cttgatcgag accttttccg tcaatcccaa
    22921 cgtcgcgttc aaactgaccg acaagctttc tttggccgcc ggtttcagcg tcatgtactt
    22981 cgatttgacc ctggatcgga aaatcccgac gggccaaccg ttctggcctt ttcccatgcc
    23041 gtgggtgggg gacgtggatg ccgacctgac gggggacagc ttcgggtacg gttggaacgt
    23101 cgcgctgcat taccagccgt gtgaatacgc caagttcggc attgcctatc gaagccaggt
    23161 gaagcagaac ttgtcgggat ccgccaagtt caccattccc gccgtcggcg agaacatcta
    23221 tcgaccgttc ctgcacaata cggacgccac gggggccgtg accctgcccg atgaagtcta
    23281 tgtgggcctt gccgtatacc cgatgaagaa cttgagcctg gaattgggag gcgtttggac
    23341 cggttggagc gactattccc agttggtcat tgcctacgac aggccggtgg cgggccgcaa
    23401 ggtgagcgtg gtttccaagg actggcacga cgtgtggaga ctcaacttcg ggatcgagta
    23461 caagcccttg aactggttgg atctccggtt cggctacgtg tttgacgagg agcccattcc
    23521 cgaccgcacg gtggattatc tggtccccgc caacgatcgt catcttttca gctttggccc
    23581 cggttttcat tggaacaact ggaccctgga cgtttcctac acctacctgc tcattaccga
    23641 ccgggatgtg gaagggagac ctttggacgg cgtctttacg ggtaagttcg aaaacggaga
    23701 cgcccacatg gtgggtgtca gtctgggcta caagttttga
//
LOCUS       DAOOVC010000052        23680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1464_length_23680_cov_6.142646, whole genome shotgun sequence.
ACCESSION   DAOOVC010000052 DAOOVC010000000
VERSION     DAOOVC010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23680
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1464_length_23680_cov_6.142646"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2797
                     /locus_tag="PLM79_08675"
     CDS             <1..2797
                     /locus_tag="PLM79_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86423.1"
                     /translation="EVFGVPNPIRVIPNFVNWRFVETFGYTLDEIPTADDWFRLAYPD
                     EDDRRKVRASWEAERDRLLLPGAKPLPHEFAVRCKDGTTKIVEFTQNILGNRMLILFN
                     DVTERRRAEESLRLHSKRVEALLKLHQMTDSPLGEITDFAVEEAVRLTGSTIGFLALM
                     NEDETVLTVNAWSRSVQVECALEEKHAHFRIAGTGLWGEAVRRRGVVLINDCRASIPW
                     KKGFPPGHVPVSRFMSVPVFSESRIVLVAGVGNKAVDYDETDVRQLTLLLEGTWSLIE
                     RKRAQEELAETQTLLHMATDASPSGIVVADAPDVRIRMVNRAAYLIRGDSPSPLNGIS
                     VEHHSENWQIHSPDGRLYDPLDLPLSRAVLKGERVENELAFIRREDGSERWVLISASP
                     IRNDRDEIVGGLAVFPDISELRQAQEALRESERKYRDLADNLPQALFETDPTGRVTYA
                     NRFVMDLFQYSPTDLERGIHALDVLSPADHSRAAEEIHKIMAGETEEFTGEFTAVKAD
                     NQEFPVIVHVGPIRRGGVVVGMRGTLIDMTERKRLEDQLLQAQKMEAIGTLAGGIAHD
                     FNNILMGIQGYASLLNLKLDSGDARHEYIQRIEQQVRSASDLTRQLLGFARGGRYEVK
                     SLDLNAILEQSSTMFGRTKKELSIHRSFQPNLWPVEADRGQIEQALLNLYLNAWQAMP
                     GGGDLCLETANVVLQEEQVRAHGLEPGRYVRVSIADTGSGMDRKTRERIFEPFFSTKA
                     LGRGAGLGLAMVYGIVKGHGGFVTVDSEPDRGSVFVLHLPASEKPIVQDKGVLPGIAV
                     GSETILLVDDEPVVREVNQEILEKLGYKVFGAGGGREALEVLAANQGGIHLVILDMVM
                     PGMSGEETFLRIKELDPKIKVILSSGYSLAGEAQRIMEKGCDGFLQKPFTTAGLSQEI
                     RKVLGRAGSDPGTGQES"
     gene            2794..3582
                     /locus_tag="PLM79_08680"
     CDS             2794..3582
                     /locus_tag="PLM79_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86424.1"
                     /translation="MMRILILAALPQEHGPYRRLSRGWRLISRRPLLEYARRDAAGEL
                     RLVETGMGGTGLGDALAHWRNAPPPDAVISMGFCGSMESTFPVGQVILGTSFQRADPS
                     REPSLSPALRLRPPAEFEHFCSLYGVRPARVITLDRPAAKGLLKSLSPGPGLVADMES
                     FHIGRFAMELDRPFLCLRSVSDASVHEIDFDLGDLTDSRGRVRIARVLRSVGKRPAVV
                     GPFLQSWRRSRTAGRSLGRVLGALLDGSPSSLHGMIEGCRLSLS"
     gene            complement(3508..4443)
                     /locus_tag="PLM79_08685"
     CDS             complement(3508..4443)
                     /locus_tag="PLM79_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOV86425.1"
                     /translation="MLDLLRLLYGTVLLRPYVFAFLAVYLVAGAAHLGVGRTLLFIPL
                     GYALAWISEFSSIHCGFPYGDYYYLETTAGRELWVFGVPFMDSLSYVFLAYCSYSTAL
                     FVLSPIRSARRNLLVLETRALRRSPQTLVLGAFLFVLLDIIIDPVALRGSRWFLGQIY
                     GYRSPGLYFGIPLSNFGGWLLVGFLEVGALQFLAARDGLEPDGPSRLSRIPGIQCLGP
                     LLYASVLVFNLTVTFWIGETFLGLVGCMILFFPSLLVFFFTLHKQRCRDPESWGEHLA
                     DFPHSPARELLSGKAESTALDHPVQGGRGTVQQGP"
     gene            complement(4436..5431)
                     /gene="hpnH"
                     /locus_tag="PLM79_08690"
     CDS             complement(4436..5431)
                     /gene="hpnH"
                     /locus_tag="PLM79_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HOV86426.1"
                     /translation="MRFPASLYASMTRYLLANKLRGNDRFPLVLMLEPTHRCNLTCSG
                     CGRIREYHDTLHREMPLDDCLQAVQECGAPVVTVTGGEPLLYDPVDRLVREILARKKH
                     LYFCTNGILLEESLSRFRPHSRFTWNVHFDGTESVHDRIIRREGGFQKALAGVRAAKK
                     RGFRVSTNTTIYRETDVRDVELLFAQLTRAGVDGILVAPGFSYEEVIRDVFMTRREIQ
                     EKFRHIVQWQDRYPLISNPIYLDFLCGKRHFLCTPWGNPTRNPRGWKSPCYLITDTHY
                     PTYRRLMEETPWEHYVSGKDPRCAQCMMHCGYEPTVVRELKGRDLLRMIRWNLYA"
     gene            complement(5501..6490)
                     /locus_tag="PLM79_08695"
     CDS             complement(5501..6490)
                     /locus_tag="PLM79_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOV86427.1"
                     /translation="MPIAFVTGATGFIGSHVALCLLENGFTVCALARPGSRHPDLAAA
                     RLEWRTGDIRSPHEVQEAMEGCDVVFHVAADYRLWCRNPREIYETNVGGTRNVLDAAR
                     IHGIERVVYTSSVGALGLDPHGAPADETVQVCLEDMVGHYKRSKFLAERCAEEFVRAG
                     LPVIMVHPSTPVGPGDRKPTPTGKIIVDFLNGRMPAYLNTGLNLVHVRDVAEGHRLAL
                     EKGIMGEKYILGNRDLTLAEIFGLLEKISGIRAPRILLPYAPILLLARINDRISRAVG
                     VEPLIPLEGVRMARKCMFFNPGKAVRELGLPQTPVETALAEAVSWFRSHGYAP"
     gene            7147..10227
                     /gene="fdnG"
                     /locus_tag="PLM79_08700"
     CDS             7147..10227
                     /gene="fdnG"
                     /locus_tag="PLM79_08700"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7717..7719,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOV86428.1"
                     /translation="MKLTRRDFLKISGAATVGLALEGMGLNLKPVEAYTATLRIKDAK
                     ETPSICCYCSVGCGILFHTDTKTGKVIYSEGDADHPINQGSLCAKGASLYQLAQNDKR
                     STKVLYRAPNSDKWQEKTWEWAYKEIAKRVKKTRDATFTVKNEKGQVVNRCNGIASVG
                     SAAMDNEECWIYQAMLRALGLVYVEHQARIUHSATVAALAESFGRGAMTNHFIDFANS
                     DVVLIMGSNAAENHPISFRWVMKAKDKGGIVIHVDPRYTRTSTKADIWAPLRCGTDIA
                     FLGGMIKYVLENDKIFKDYVVNYTNAAYIVGEKFDFKDGLFSGFNAEKKAYDASNWAY
                     VLDEKGIPKKDLSLQDPRCVLQLLKKHYSRYDLDTVSKITGTPKEDLLKVYEAYSSTG
                     KPDKAGTVLYAMGWTQHTVGVQNIRAMCILQLLLGNMGIAGGGVNALRGESNVQGSTD
                     QGLLFHILPGYLPTPSASVTSLAEYNKKYTPVSKDPRSPNWWQNRPKYMVSFLKAMYG
                     DAAKPENEFCYSWLPKTEDGKSYSWLDLFDAMYKKELKGFFSWGQNPACSGANSNKTR
                     EAMANLDWMVVVNLFDNETASFWKGPGMDPKKIKTEVFYLPCAASMEKEGSISNSGRW
                     TQWRYKAQEPPGKALPDGDIIAELFLEIRKLYQKPDKKTPLPDPIVNLKWDYMTNHKF
                     DPHLVAKAINGYFLKDVTIGDKTYKKGDLVPAFAMLKDDGSTSCGNWIYCQSYNQDGN
                     NMARRDKSDPSGIGLFPKWSWAWPVNRRIIYNRASVDPTGKPWNPKRVVIEFAGEEKD
                     GKYTSMKWKGDVPDGPWYPLKNPDGSDREDGKLAFIMKPDGYASIFGPGLKDGPFPEH
                     YEPMECPVEKNPLGAQLTNPVAAVYTTAKDALKTCDPRFPFVGTTYRVTEHWQTGVLT
                     RWIPWLIEAEPQIFVEMSEELAQLRQIKNGEKVVVESPRGSVEAVAIVTKRFRPLTVQ
                     GVEIHQVGLPWHYGWVWPKEGGDSANLLTPSVGDPNTRIPESKAFMVNVRKKGV"
     gene            10231..10962
                     /locus_tag="PLM79_08705"
     CDS             10231..10962
                     /locus_tag="PLM79_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HOV86429.1"
                     /translation="MAGKSIFIDTTRCTACRGCQVACKNWNRNGTSATKNTGSHQNPP
                     DLDENTFKLVRFSEVVTDGKLAWYFFADQCRHCLEPPCKDSIEGYVDGGVVQDEATGA
                     VVYLDKSKEAPFDEVRSSCPYDIPRKKADGVMVKCTLCIDRISNGLQPACVKICPTGA
                     MNFGERDEMVALAKDRLEKIKAKNPKAQLLDADSVRVIFLVVDDPKKYHKNAVASASP
                     GITRKVALGRMLKPAKGLLRTLTQG"
     gene            11371..12267
                     /locus_tag="PLM79_08710"
     CDS             11371..12267
                     /locus_tag="PLM79_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HOV86430.1"
                     /translation="MKPNLKAEKDRIRRALDLARAQRPAYKELLPLLEALFELQIHAK
                     ETLNLPSKELSSEWVKTRWENGFPLLNRWEFPVDFDAADRIFWGIREHIPPANPQLRK
                     GWEILSRAFEKAPSEKPSVWGSFLQHENEPWQMWVDMEESELAPVLFLARSCLRPSIE
                     SVEADLVSRFPVPRDWFKGYCPVCGSLPSLLYLIGQGERWATCSWCGAEWVLHRLQCC
                     YCDNRAHESLGYLYIEDEPGYRVQYCRLCKFYFKTVDLRERDDNRYFPLEDWTTLHLD
                     LLAQEAGWRQAPSPSPTVYPQP"
     gene            12597..14489
                     /locus_tag="PLM79_08715"
     CDS             12597..14489
                     /locus_tag="PLM79_08715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV86431.1"
                     /translation="MAEKSWKTYYRERLGTAEEVLKTVLRDGNRIYLGSACGEPQHLA
                     RALVSLLPAYRDLELVQNLSLGSLPEDWSQLHEHCRLKTFFLGNRTRRAVNSGFGDYV
                     PMYFSSIPGLLRDEVSWSLDLCLIQVSPPDEHGFCSLGIAVDINKVAAEMAKIVIAQV
                     NPQMPRTLGDSFIHVNRLDYFVEHEEPLIEVFFRERSAVAERIARYVSALIEDGSTVQ
                     VGIGRIANSILRYLDDKKDLGVHTEILTDAHLELVKKGVITSRRKSLHVGKIISSCCM
                     GSRRLYEFVHNNPEVELYPTEYVNDRAVISQNDQMVAINSALEMDLSGQVCADSIGPM
                     VYSGIGGYVDFMHGATLSPRGKTIVVLPSTSPDGRRSRIVRHLTEGAGVVSSRSTVRY
                     VVTEFGIAYLHGKTIRERALALINIAHPKFRERLLAEAKHLRYVYQDQILPPIYEPLY
                     PGQWETYQIFPGNVMIHFRPIKPTDERAIQEFFYTLPDQDIYYRFLSAMKVFPHRNTQ
                     GMCNIDYEHEMAIVGVIGDIGNETVLALGRYILDQKTNMAEVDFAVRAEWQRRGIGTF
                     LLHYLCEIAKSKGISGLTAYVLAANRSMLAVFHKVGYCIHSHFEEGVHEISFRFDEPA
                     QACITE"
     gene            14599..>14910
                     /locus_tag="PLM79_08720"
     CDS             14599..>14910
                     /locus_tag="PLM79_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86432.1"
                     /translation="MHPSGTQPATLEEGFGPEFWGRIPPHQVAFDIDGVVADTMAVFV
                     RLARERYGLSHVTKADLRVYDLYQCLDVDREIIRDLICLTLDDQHTLETPPMPGAREV
                     LT"
     assembly_gap    14911..15010
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15011..15404
                     /locus_tag="PLM79_08725"
     CDS             <15011..15404
                     /locus_tag="PLM79_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86433.1"
                     /translation="DQHTLETPPMPGAREVLTEISRVAPLRFITARIWPESIMEWLDR
                     LLPRVGPGQIRVVATGSPEAKLQILKDLGVRYFLEDRVETCRLLASDGIQPLLFDQPW
                     NRGPEACGLPRIESWSHFRRMLLLNNGE"
     gene            15522..16190
                     /gene="minC"
                     /locus_tag="PLM79_08730"
     CDS             15522..16190
                     /gene="minC"
                     /locus_tag="PLM79_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOV86434.1"
                     /translation="MSSNEPLPIQIKARKDGFVLVPDAEASFETILGFLQQRLEEARD
                     FFHHAQMILDLRERPLRTDEIRSLQCLLMDKVGARITQIRLDEETSYFASPGAGGSVP
                     NPRSVSMPEPVPESTPMIVHSTCRSGTRVESGSDCVILGDVNPGAEVLAVGDVIIFGN
                     LRGIAHAGAAGNRRARIWALSIEPSQIRIADLVAVPPKGNKPVPKRYEVAEIRKDLIE
                     VITV"
     gene            16322..17092
                     /gene="minD"
                     /locus_tag="PLM79_08735"
     CDS             16322..17092
                     /gene="minD"
                     /locus_tag="PLM79_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOV86435.1"
                     /translation="MTSGKGGVGKTTTVANIGTALAQKKHSVVLIDADIGLRNLDVVM
                     GVENRIVYNLVDVIEGRCRKQQAMIRDRKFNNLYLIPAAQTRDKDSVTPEQMKDLCTE
                     LAEEFDFVLIDCPAGIEQGFRNAIAGAQKALIVTTPEVSAIRDADRVIGLLEASLLRD
                     IHLIINRLNHRMVKKGDMMSTADIISLLSVPLLGVIPESEDVVISTNRGVPLVHERNS
                     KAGKALQRIAARLNGEDVPLDDGQGDGFMDRVKRFIWS"
     gene            17109..17378
                     /gene="minE"
                     /locus_tag="PLM79_08740"
     CDS             17109..17378
                     /gene="minE"
                     /locus_tag="PLM79_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOV86436.1"
                     /translation="MLRAIRRFFGEKASGQLARKRMQVVLMHDRLDLSPEILDALKND
                     IITVLSRYMEIDRNTIRVDLEQGKDYAALVSNVHVKRVFRRAESV"
     gene            17403..17642
                     /locus_tag="PLM79_08745"
     CDS             17403..17642
                     /locus_tag="PLM79_08745"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOV86437.1"
                     /translation="MALQVRLGATLRTYVHPYDGSVGYEMVVPPGTTVRELVRRLKIP
                     EAEVRLIFVNGVSSGWETVLAGDERVGLFPPVGGG"
     gene            17932..19110
                     /locus_tag="PLM79_08750"
     CDS             17932..19110
                     /locus_tag="PLM79_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV86438.1"
                     /translation="MRALVVEDHNQMRMMVRGMLEETKRFELVDEAADGERGWERILD
                     LWTHSSDVYDLVVCDVNMPVLGGLELLKRCRSRAEFRFLPFIMISTAPSESTIAQALG
                     EYGANDFLVKPLCSKLFEQRVLLVLSRAQSPEEVLYRRAESLKESDAVREALELIESW
                     EKENRFSLAKWFNLKGECLFKAGDPDKAALEFQKATQLCEIYVAAYKNYASAQQKLGN
                     TEKVIEALAHIEAISPSDDSRTLLLGELLFKAGRPEEGKRYLENLLKRSNRNEKDKIV
                     RKTAQLYLDAGLYQEAEGLFKMTLKSPLADVETYNQLGIALRQQGKFEEAERCYLTAL
                     NTYPRHAGIYHNLGVLHMARRDYSKARRSFQKALIFNPHLQESKDMIEKIELLDREPT
                     "
     regulatory      19257..19442
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            20035..20634
                     /locus_tag="PLM79_08755"
     CDS             20035..20634
                     /locus_tag="PLM79_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86439.1"
                     /translation="MEFQGVAQWLGYFLGTLSAVVIQGEGAAYFVLMFEPISRRGKVI
                     YDLNPAHHLDLRSIPLLFLAGWTWSKKCVVKPDYFPNSRISGALTGLAGGLAILLMSG
                     ILGTLYMFLPFGALETAIRACVVIAMANLLIPIPPFALGRALWCPFQDTLKHQKAVEW
                     AGAIAVTALVLLEVWMKSPFLTDRIFSPASFLSRWILSG"
     gene            complement(21284..21526)
                     /locus_tag="PLM79_08760"
     CDS             complement(21284..21526)
                     /locus_tag="PLM79_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86440.1"
                     /translation="MDGNHRRTDWGRIVVVILLVASMTGCAGMSQREQRFLSGGAIGT
                     AAGVGTAAIVGAPLIVGGAVGAAAGAVGGLIYDEVR"
     gene            21859..23472
                     /gene="pckA"
                     /locus_tag="PLM79_08765"
     CDS             21859..23472
                     /gene="pckA"
                     /locus_tag="PLM79_08765"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOV86441.1"
                     /translation="MEFDDLIPDHQLDHVGLYQLRHVYWNRSTPELYELAIRRYEGQI
                     AHLGPLVVSMGQHTGRAAKDKYVVDEPTTTDEIWWGKVNVKYPEERFHTLHKRMTNYL
                     RGKTVFVQDCYAGADERHRQSVRVVTEFAWHSLFARNMFIQLPRDVAQIKSFVPDFTV
                     IHCPNFHADPEDDETRSGTFVALHLSKKLVLIGGTAYAGEIKKSIFTALNFLLPAQDV
                     LSMHCSANVHKDNPEDVAVFFGLSGTGKTTLSADPKRLLIGDDEHGWSENGIFNFEGG
                     CYAKVINLSKSSEPEIYECTRRFGTILENVSMSPVIRRLDLDDARLTENTRASYPLSH
                     LPNIVPSRTAGHPRTVVMLTADAFGVLPPISRLTEEQAMYHFISGYTAKVAGTEKGVK
                     EPGATFSACFGAPFMVRHPSVYAQMLAKKIRDHKVGCWLVNTGWTGGPYGVGSRMKIE
                     LTRALLNAALAGDLNHVPMKPHPVFGVLVPTESPGVPSELLDTRRTWSDPERYDAQAR
                     RLAVMFRENFEQFKDQASPEVRAAGPRAD"
BASE COUNT         4862 a         7172 c         7076 g         4470 t
ORIGIN      
        1 ggaagtgttc ggtgtgccca acccgatccg ggtgatcccc aatttcgtga actggcggtt
       61 tgtcgagact ttcggctata ccctggacga aatccccacg gccgacgact ggttccgcct
      121 cgcctacccc gacgaagacg accgcaggaa ggtacgggcc tcgtgggagg cggagcggga
      181 ccggctgctc ctccccggcg ccaagcccct gcctcacgaa ttcgccgtgc gatgcaaaga
      241 cggcacgacc aagatcgtcg agttcaccca gaacatcctg ggaaaccgca tgctcatcct
      301 cttcaacgac gtgacggaaa gaagacgggc cgaggaatcc ctgaggcttc attccaaacg
      361 cgtggaggcc ctgctcaaac tccaccagat gaccgactcg cccctggggg agatcaccga
      421 ttttgccgtg gaggaggccg tgcgccttac gggcagcacc atcgggttcc tggccctcat
      481 gaacgaagat gagaccgttc ttaccgtgaa cgcatggtcc cgctcggtcc aagtcgaatg
      541 cgccctggag gagaagcacg ctcatttccg catcgcaggg acggggctct ggggggaggc
      601 ggtgcggcgg agaggggtcg tcctcatcaa cgattgccgg gcatcgatcc cttggaagaa
      661 gggatttccc ccggggcacg ttcccgtttc ccgcttcatg agcgtccccg ttttctccga
      721 gtcccgcatc gtcttggtgg ccggggtggg aaacaaagcc gtggactatg acgaaacaga
      781 cgtccggcag ctcaccctcc tgttggaagg cacctggtcc ctcatcgaac ggaagagggc
      841 ccaggaggag cttgccgaaa cccagactct cctccacatg gccaccgatg cgtccccctc
      901 cggcattgtg gtggccgacg ccccggacgt caggatccgc atggtcaacc gcgccgccta
      961 tctcatacgg ggggattcgc cgtcccctct caacgggatt tccgtggaac atcattccga
     1021 gaactggcag atccattcac ccgacggcag gctttacgac cccttggacc tgcccctctc
     1081 ccgggcggtc ctgaaaggag aacgggtaga gaacgaactg gcgttcatcc gccgcgagga
     1141 cggctccgag cgatgggtcc tcatcagtgc ttcccccatc cgcaatgacc gtgacgagat
     1201 cgtgggcggt ctggcggtct tccccgacat ctcggaactg aggcaggctc aggaggccct
     1261 tcgggaaagc gagaggaaat accgagacct ggcggacaac ctgccccagg ccctcttcga
     1321 aaccgacccc accggccggg tcacctatgc caaccgtttt gtcatggatc tttttcagta
     1381 tagccccaca gacctcgaac gcggcatcca cgccctggat gtcctctcac ccgccgatca
     1441 ttccagggcc gccgaggaga tccataagat catggcgggg gaaacggagg agttcacggg
     1501 ggaattcacg gccgtgaagg ccgacaacca ggagtttccc gtcatcgttc atgtgggccc
     1561 cattcggcgg ggcggggtcg tcgtgggcat gagagggacc ctcatcgaca tgaccgagcg
     1621 caagaggctc gaagatcagc tcctccaggc ccagaagatg gaggctatcg gcaccctcgc
     1681 aggaggcatc gctcacgact tcaacaacat cctcatggga atccagggat acgcctccct
     1741 gctgaacctc aagctggatt ccggagatgc ccgccacgaa tatatccaaa gaatcgagca
     1801 gcaggtgcgc agcgcctcgg acctcacccg gcaactcctg ggattcgccc ggggcggacg
     1861 atacgaggta aaatccctgg atctcaacgc gattttggaa cagagctcga ccatgttcgg
     1921 caggaccaag aaggagctct ccatccaccg gagcttccag ccgaaccttt ggcccgtgga
     1981 ggccgaccgg ggccagatcg agcaggcgct cctcaacctt tatctcaacg cctggcaggc
     2041 catgcccggc gggggagacc tctgcctgga aacggccaac gtggttctcc aggaggagca
     2101 ggtgcgggct cacgggctcg agcccggacg gtacgttcgg gtgtccatcg ccgatacggg
     2161 ctcgggtatg gaccggaaga cccgggaacg gatcttcgag cccttctttt ccaccaaggc
     2221 cctgggccga ggagcgggcc tggggctggc catggtctac ggcatcgtca agggccacgg
     2281 cggcttcgtt accgtggaca gcgaacccga tcgcggttcc gttttcgtcc tccaccttcc
     2341 ggcttccgaa aagcccatcg tgcaggataa aggagtcttg ccgggcattg ccgtcggttc
     2401 ggagaccatc cttctcgtgg acgacgagcc cgtggtgcgg gaggtgaacc aggaaatcct
     2461 cgagaagctc gggtacaagg tcttcggggc gggcggcggc cgggaagcgc tcgaggtgct
     2521 ggccgcaaat caaggcggca tccacctggt catcctggat atggtcatgc cgggaatgtc
     2581 gggggaggaa accttcctgc gcatcaagga gttggacccg aaaattaagg tcattttgag
     2641 cagcgggtac agccttgcag gagaggctca aaggatcatg gagaagggct gcgacggctt
     2701 tttgcagaag cctttcacca ccgccgggct ctcccaggaa atccgaaagg ttttgggtag
     2761 agcgggttcc gatcccggca cgggacaaga atcatgatga gaatcctcat cctggcggcg
     2821 ctcccccagg aacacggacc ctaccggagg ctctcccggg gctggcggtt gatctcccgg
     2881 cgtccccttc tcgagtacgc ccgaagggat gccgccggag aacttcgcct ggtggaaacg
     2941 ggaatggggg ggaccggcct gggagacgcc cttgctcact ggaggaatgc accccctccc
     3001 gatgcggtga tctccatggg gttttgcggc agcatggaga gtactttccc cgtgggccag
     3061 gtcatcctgg ggaccagctt ccaaagggcg gacccctccc gggagccttc cctttccccg
     3121 gcccttcggc ttcggccccc ggcggagttc gagcatttct gttctcttta cggagtacgc
     3181 ccggcccggg tgatcaccct ggaccgtcct gcggccaagg gccttctcaa aagcctttcc
     3241 cccggtcccg gtctcgtggc ggacatggag agtttccata tcggccgatt cgccatggaa
     3301 ttggaccgtc ccttcctctg ccttcgaagc gtcagcgacg cgtccgttca cgagatcgat
     3361 ttcgacctgg gggatctcac cgactcccgg ggacgggtga gaatcgcccg ggtcctgcga
     3421 tcggtgggaa aaagacccgc cgtggtcggc cctttccttc agtcctggcg ccgttcccga
     3481 actgcgggcc ggtcgttggg aagggtccta ggggccttgc tggacggttc cccgtcctcc
     3541 ctgcacggga tgatcgaggg ctgtagactc agcctttcct gaaagcaact ccctcgcggg
     3601 agaatggggg aaatccgcca ggtgctcccc ccaagactcg gggtcccggc aacgctgttt
     3661 gtgaagggtg aaaaagaaga ccagcagcga ggggaaaaac aggatcatgc atcccaccag
     3721 tcccaggaac gtttccccga tccagaaggt gacggtgagg ttgaagacca ggacacttgc
     3781 atacaggagc ggacccaggc attgaatgcc gggaatgcgg ctcaaacggg acgggccgtc
     3841 cggctcgaga ccatctcggg ccgccaggaa ctgcagggcc cccacctcga gaaacccgac
     3901 caggagccag cctccaaaat tgctcaaggg aatgccgaaa tagagcccgg gagaccggta
     3961 tccgtaaatc tgccccaaga accaacgaga tccccggagg gccacggggt cgatgatgat
     4021 gtccagcaga acaaagagga aggcccccag gaccagggtc tggggggaac ggcgcagtgc
     4081 gcgcgtctcg aggaccagca gattcctccg agccgaccgg atggggctca ggacaaacag
     4141 ggccgtggaa taactgcaat aggccaggaa gacataggaa agggaatcca tgaaggggac
     4201 cccgaaaacc cagagctccc ggcccgcggt ggtctcgagg tagtaatagt ccccgtaagg
     4261 aaagccgcaa tggatggagg aaaactccga gatccaggcg agggcatatc ccagggggat
     4321 gaacagcagg gtcctgccca cccccaggtg ggccgcgccc gccaccaggt acaccgccag
     4381 aaaagcgaag acataggggc gcaggagaac ggtcccgtag aggagccgca gcagatcaag
     4441 catagaggtt ccatcgaatc atgcgaagaa ggtctctccc tttgagttcg cgcaccaccg
     4501 tgggctcgta tccgcagtgc atcatgcatt gagcgcaccg agggtccttg ccggacacgt
     4561 agtgttccca gggagtctcc tccatcaacc ggcgataggt gggatagtgg gtgtccgtaa
     4621 tgagatagca gggggatttc cagcctcggg ggttccgggt ggggttgccc cagggagtgc
     4681 aaagaaaatg gcgctttccg cacaggaaat ccaggtaaat gggattgctg atgaggggat
     4741 atcgatcctg ccattgaacg atatgccgga atttctcctg gatttcccgg cgggtcatga
     4801 aaacgtcccg gatgacctcc tcgtaactga agccgggggc caccaggatc ccgtccacgc
     4861 ccgcccgggt gagctgagcg aatagaagct ccacgtcccg gacgtccgtt tcccggtaga
     4921 tggtggtgtt ggtgctcacc ctgaaacccc gcttcttggc tgctcgaacc cccgccaggg
     4981 ctttttggaa gccgccttcc ctccggatga tccggtcgtg aacggactcg gttccgtcga
     5041 aatggacgtt ccaggtgaag cgcgagtggg gccggaacct cgatagggat tcctccagga
     5101 gaatcccgtt ggtgcaaaag tagaggtgtt tcttgcgggc caggatttcc cgcaccagcc
     5161 ggtcaacggg gtcgtagagc aggggttcgc ccccggtgac ggtcaccacc ggggccccgc
     5221 actcttgtac ggcctgcaga cagtcgtcca ggggcatttc ccggtggagg gtgtcgtggt
     5281 attcccggat gcgcccgcac ccggaacagg taagattgca tcggtgggtg ggctcgagca
     5341 tgagaaccag ggggaatcga tcgttgcccc ggagcttgtt ggcgagaaga tagcgggtca
     5401 tggacgcgta cagactggcg ggaaatctca tccggttcct tttcgtgtgg aagcgcgtga
     5461 aacgcgcgtc aaaagcgttt tcccaaggct cagcccgggg tcagggagca tacccgtggc
     5521 tgcggaacca gctcaccgct tcggccaggg cggtttccac cggagtctgg gggagcccca
     5581 gctcccgcac ggccttcccg ggattgaaaa acatgcactt gcgggccatt cggaccccct
     5641 cgaggggaat gagcggctcc actcccacgg cccgtgaaat ccggtcgttg atccgggcaa
     5701 gaaggagaat gggcgcatag ggcaggagaa ttcggggagc gcggattccc gagatcttct
     5761 cgaggagccc gaagatttcg gccagggtaa ggtcccggtt gcccaggatg tatttttccc
     5821 ccatgattcc cttttccaag gccaaacggt gcccctcggc cacgtcccgc acgtggacca
     5881 aattgagccc cgtgttgagg taggcgggca tccgaccgtt gagaaaatcc acgatgatct
     5941 tgcctgtggg ggtgggctta cggtctccgg gtccgacggg agtgctgggg tgcaccatga
     6001 tcacgggcag ccccgccctc acaaattctt cggcacaccg ctcggccagg aacttggacc
     6061 gtttgtaatg gcccaccata tcttcgaggc agacctgaac ggtctcgtcg gcgggagccc
     6121 cgtggggatc gaggcccagg gcgccgacgc tgctggtgta gaccacccgt tcgatcccgt
     6181 ggatacgggc cgcatcgagg acgtttcttg tgccccccac gttggtttca tagatttccc
     6241 gggggtttcg acaccagagg cggtaatcgg cggccacgtg aaacaccacg tcacagcctt
     6301 ccatggcttc ctgcacttca tgtgggctgc gaatgtcccc ggttctccac tccaaccggg
     6361 ccgcggccag atcgggatgg cgggagccgg ggcgggccag ggcgcaaacg gtgaacccgt
     6421 tttccaacag gcataaggcc acgtgggacc cgatgaatcc cgtggcaccc gtcacaaagg
     6481 caatgggcat ggtcgtttcc aggatccggt tttcttggag gacttttccg cagggatgtc
     6541 gcccggcggg accgaacggg ccccccgagg ccggtgcccc ccgcttcttc cgggtcccct
     6601 tataacaaaa agacctttgc aatctccagt cttaaattcc ccgggaccgg gggacccgag
     6661 actcctttgg ggcgggtccg tgagggagga cctcccccgc ccgacggtca cggctttgcg
     6721 gggcctccgc agggagccgc cgtgttctcc cgcccatgct ggaccggacc cggagaggga
     6781 aagggacggt ccgtcgacgg gaaaagtccc gcgtgtttcg gggtttgacc acccggaatc
     6841 cggtttgatc cagtttgatg gttgacatgg gaacggggct acagtatcgt gcaaccggct
     6901 tgcagtatgg atttctgact gtattaccgg actctgcaac tccatctttt ctggttcccc
     6961 ctgcaccggg atgcaccgtt gtgcaccccc gcaccgaaaa gctgaatcct cacaggtagg
     7021 gtatcgctgc agtcgttttc gttagtggaa aaatttcaca aggaggaggt gagaccaaag
     7081 tcacgaccga cacaaccagg ttttcccgaa tgccgagctg acaaacaatg agggaggaca
     7141 gaagacatga agctgactcg aagggatttc ctcaaaatct ccggggccgc aacagttggg
     7201 cttgccctgg aggggatggg gctcaatctc aaacccgtgg aggcctatac cgccacactg
     7261 cgcatcaagg acgccaaaga gactccgagc atttgctgct attgctccgt gggttgcgga
     7321 atcctgttcc acacggacac caagaccgga aaagtgatct attccgaagg ggacgcggat
     7381 catcccatca accaggggtc cctctgcgcc aagggcgcgt ccttgtacca gctggctcag
     7441 aacgacaaac gaagcaccaa ggtcctgtac cgggctccca acagcgacaa gtggcaggaa
     7501 aagacttggg agtgggctta caaggagatt gccaaacggg tgaagaagac ccgggacgca
     7561 acctttacgg tgaagaatga aaagggtcag gtcgtcaacc gctgcaacgg cattgcatcc
     7621 gtgggcagcg cggccatgga caacgaggaa tgctggatct accaggccat gcttcgtgcc
     7681 ctgggactgg tctatgtcga acatcaggcc cgtatttgac acagtgccac ggtagcggct
     7741 ctggcagagt cgtttggacg cggcgccatg accaaccatt tcattgattt tgccaacagt
     7801 gacgtcgtcc tgatcatggg cagcaacgcc gccgagaacc accccatctc gttccgctgg
     7861 gtgatgaagg ccaaggataa gggaggaatc gtgatccacg tggatccgcg ttatacccgc
     7921 acctccacca aggcggacat ctgggcccct ttgcgttgcg gcacggacat cgccttcctg
     7981 ggcggcatga tcaagtacgt cctcgaaaac gacaagattt tcaaggacta cgtggtcaac
     8041 tacaccaacg cggcttacat cgtgggggag aagttcgact tcaaggacgg cctcttctcc
     8101 ggcttcaacg ccgagaagaa ggcctacgac gcatccaact gggcctacgt cctggacgaa
     8161 aagggcattc ccaagaagga tctctctttg caggaccccc ggtgcgtgct tcagcttttg
     8221 aagaaacact attcgcgtta tgacctggac acggtttcca agatcacggg aacccccaag
     8281 gaagacctgt tgaaggtcta cgaggcctac agttcgacgg gcaaacccga caaggccgga
     8341 acggttctct acgccatggg ctggacccag cacacggtgg gagtccagaa catccgggcc
     8401 atgtgcatcc tccagctcct cctgggcaac atggggatcg ccggcggcgg cgtgaacgcc
     8461 cttcgagggg aatccaacgt gcagggttcc acggatcagg gcctgctctt ccacatcctg
     8521 ccgggctacc tgcccactcc ttcggcctcg gtaacctccc tggccgagta caacaagaaa
     8581 tacacccccg tcagcaagga tcctcgaagc cccaactggt ggcagaatcg gcccaagtac
     8641 atggtaagct ttctcaaagc catgtacggg gacgcggcca agcccgagaa cgagttctgc
     8701 tacagctggc ttcccaagac cgaggacggg aagtcctata gctggctgga cctcttcgac
     8761 gccatgtaca agaaggagct caaagggttc ttctcctggg gtcaaaaccc ggcctgcagc
     8821 ggagccaatt ccaacaagac ccgggaagcc atggccaacc tcgactggat ggtggtggtg
     8881 aacctgttcg acaatgagac cgcttccttc tggaaggggc cgggcatgga tcccaagaag
     8941 atcaagacgg aggtcttcta cctgccctgt gccgcctcca tggaaaagga agggtccatc
     9001 agcaacagcg gccgttggac ccagtggcgc tacaaggccc aggagccccc gggcaaggcc
     9061 cttcccgacg gcgacatcat tgcggaactg ttcctcgaga tccgaaagct ctaccagaag
     9121 cccgacaaga agacccccct gcccgatccc attgtaaacc tcaaatggga ttacatgacc
     9181 aatcacaagt tcgatcccca tctggtggcc aaggccatca acgggtactt cctcaaggac
     9241 gtcaccatcg gcgacaagac ctacaagaag ggagatcttg tcccggcctt cgccatgctc
     9301 aaagacgacg ggtccacctc ctgcggcaac tggatttatt gccagagtta caaccaggac
     9361 ggcaacaaca tggctcgtcg ggacaagagc gatcccagcg gcatcggcct gttccccaag
     9421 tggtcctggg cctggcccgt gaatcgaagg atcatttaca accgggcctc ggtggaccct
     9481 accgggaagc cttggaatcc caagagagtc gtcatcgaat tcgccgggga agagaaagac
     9541 gggaaataca cgtccatgaa gtggaaggga gacgttccgg acggaccctg gtatcccctc
     9601 aagaatcccg acggcagcga tcgggaggac ggcaagctcg ccttcatcat gaaacccgac
     9661 ggatacgcgt ccatcttcgg cccggggctg aaggacgggc cgttccccga gcactatgaa
     9721 cccatggagt gcccggtgga aaagaacccc ctgggcgcgc agctcaccaa ccccgtggcc
     9781 gccgtgtaca cgacggccaa ggacgccctc aagacctgcg accctcgttt ccccttcgtg
     9841 ggcaccacgt accgggtcac ggaacactgg cagacggggg tcctcacccg ctggattccg
     9901 tggctcattg aagcggagcc ccagattttc gtggaaatga gcgaggaact ggctcaactg
     9961 cgccaaatca agaacggaga aaaggtcgtg gtggaatctc cccgcggatc tgtggaggcg
    10021 gtggccatcg tgaccaagcg gttcagacct ctgacggtgc agggagtgga gattcatcaa
    10081 gtcggactgc catggcacta tggatgggta tggcccaaag aggggggaga cagcgccaac
    10141 ttgctgactc cgtccgtggg cgaccccaac acccgcattc cggagagcaa ggcttttatg
    10201 gtgaacgttc gaaagaaggg agtgtaagcc atggcgggaa aaagcatatt catcgatacc
    10261 acgcgatgca cggcctgccg aggctgccag gttgcgtgca agaactggaa ccgcaacggc
    10321 acctccgcaa ccaagaatac gggaagtcac cagaacccgc cggacctgga cgagaacacc
    10381 ttcaagctgg ttcgcttcag cgaggtggtc accgacggaa agctggcctg gtacttcttc
    10441 gccgaccaat gtcggcattg cctggaaccc ccctgtaagg actccatcga gggatacgtg
    10501 gacggaggag tcgtccagga cgaagcgacg ggagccgtgg tgtatctcga taagagcaag
    10561 gaggctcctt tcgacgaggt ccgttcttcc tgtccctacg atatcccccg gaagaaggcc
    10621 gacggggtca tggtcaagtg caccctgtgc atcgaccgca tcagcaacgg tcttcagcct
    10681 gcctgtgtga agatctgtcc caccggcgcc atgaattttg gggaacggga cgaaatggtc
    10741 gccctggcca aggaccgctt ggagaagatc aaggccaaga atcccaaggc ccagctcctg
    10801 gacgccgatt ccgttcgagt gatcttcctg gtggtggacg acccgaaaaa gtatcacaag
    10861 aacgccgtgg cctccgccag tccggggatc acccgcaaag tggccctggg ccgaatgttg
    10921 aagccggcca aagggctcct tcggaccctg acgcagggat gattcctcga gggcaagaac
    10981 ctccgcacgg cggacgacga aaagaccgtc tccgaccgtt cctcccccgt tggggggagg
    11041 tcttgcggag ggacaaaggg attgactcac cccccttctc caaccccctc ccacccgggg
    11101 aggggaggtg ggggggattt ccgcccggga ggggatccgc cgtgacggag ccgggacgaa
    11161 ggccggtcga gacgttcgtc cccggcggcg gcgaaggcgg cgcagcttcc cccccgacag
    11221 gggccgtaag caaggggttc tgccgggatt cggggaagcg caacgggctt tgccgggaga
    11281 ggaaaggttc tccccgggac gggacgcggt tgggcggagg ggggtgccct tggggtagga
    11341 aacgtcaacc tgtaaccgga aggattgtct ttgaaaccga acctgaaagc cgaaaaggac
    11401 cggattcgcc gcgccctcga ccttgctcga gctcagaggc cggcctacaa ggagcttctt
    11461 cccctcctgg aagccctctt tgaactgcaa attcacgcca aggagaccct gaatctcccc
    11521 tccaaggagc tgtcctccga gtgggtcaag acccggtggg aaaacgggtt tcccctgctc
    11581 aaccgctggg agtttcccgt ggatttcgat gccgccgacc ggattttttg gggaatccgg
    11641 gaacacattc caccggcaaa tccgcaactt cggaaaggct gggagatcct gagccgcgcc
    11701 tttgagaagg cgccctccga gaaaccctcg gtttggggaa gcttcctgca gcacgagaac
    11761 gagccctggc agatgtgggt ggacatggag gaatcggaac tggcgcccgt gctcttcctg
    11821 gcgcgtagtt gcctgagacc gtccatcgag tccgtggaag cggaccttgt ttcccgcttt
    11881 ccggtccctc gggattggtt caagggttac tgccccgtct gcggttccct tccttccctc
    11941 ctgtatctca tcgggcaggg ggaacgttgg gcaacctgtt cctggtgcgg ggccgaatgg
    12001 gtcctgcatc ggctccagtg ctgctattgc gacaatcgcg cccacgaatc cctgggatac
    12061 ctctacatcg aggacgaacc cgggtaccgt gtccaatact gtcgactctg caaattctat
    12121 ttcaaaacgg tggatctcag ggaacgggac gacaaccggt attttcccct cgaggactgg
    12181 accactcttc acctggatct cctggcccaa gaagcggggt ggcggcaggc gccctcccct
    12241 tccccgacgg tctacccgca gccctgagaa accggctcga aaccccgccc tcggccttat
    12301 ccggccttcc cggatcctcc ctcccatggg agggaatccg ggaggagagc ccggatgcct
    12361 gccctcgcga acaggacggt tgcgggggga ttctcttcca ggtcccgcca attcctgttt
    12421 ccctgtcctt tttgccgtgt aagcccatgc aaccggctcg ggccggacgt tgccctcggg
    12481 gggctcggtc ttgtgcactt gacgcggtgg agacttccct tcattatagg agaagtcctc
    12541 ctgggagcac cttctttggg aatcaaacct gtaacgggac gaataggaaa gaggacatgg
    12601 cggaaaaatc ctggaagacc tactaccggg aacgactggg cactgccgaa gaggtgctga
    12661 aaacggtttt acgggacggg aatcgaatct acctggggtc ggcctgcggg gagccccagc
    12721 acctggcccg cgccctggtg agtcttctgc ccgcttaccg ggacttggaa ctggttcaaa
    12781 acctttccct ggggagtctt cccgaggact ggagtcagct ccacgagcac tgcaggctga
    12841 agaccttttt cctgggaaac cgcacccgcc gcgccgtcaa cagcggcttc ggggactacg
    12901 ttcccatgta cttctcttcc attccgggac tgctgcgcga cgaggtgtca tggagcctgg
    12961 atttgtgtct catccaggtc tctcctcccg acgagcacgg gttttgttcc ctgggcatcg
    13021 ccgtggacat caacaaggtg gccgccgaga tggccaaaat cgtcattgcc caggtgaacc
    13081 cccagatgcc gcgcaccttg ggtgacagct tcatccacgt gaaccggctg gattatttcg
    13141 tcgagcacga ggaacctctc atcgaggtct ttttccgcga acgaagcgcc gtggccgaac
    13201 ggattgcccg ttacgtctcg gccctcatcg aggatggatc caccgtccag gtggggatcg
    13261 ggaggatcgc caattccatc ctgcgatacc tcgacgacaa gaaggacctg ggcgttcata
    13321 cggaaatcct tacggacgcc catctggaac tggtgaaaaa gggggtcatc accagccgac
    13381 gcaagtctct ccatgtgggc aaaatcatct cgagttgctg catgggcagt cggaggcttt
    13441 acgaattcgt tcataacaac ccggaggtgg aactctaccc cacggaatac gtgaacgacc
    13501 gggccgtgat ctcccaaaac gaccagatgg tggccatcaa ttcggccctc gagatggacc
    13561 tcagcggaca ggtgtgtgcg gattccatcg gccccatggt ctacagcgga atcggcggat
    13621 acgtggactt catgcacggc gccaccctct ctccccgggg caaaaccatc gtggtgctgc
    13681 cgtccaccag tcccgacggt cgccgttccc gcatcgttcg acacctgacc gagggggcgg
    13741 gtgtggtcag ctcccgcagt accgtgcgat acgtggtcac ggaattcggg attgcctacc
    13801 ttcacgggaa aaccattcgg gaaagggcac tggccctcat caacatcgca catcccaagt
    13861 tccgcgaacg cttgctggcg gaagccaagc accttcgcta cgtttaccag gaccagatcc
    13921 tgcctcccat ctacgaaccc ctgtatccgg gtcagtggga aacctaccag atttttccgg
    13981 ggaacgtcat gatccatttc cgccccatca aacccacgga tgagcgcgcc atccaggagt
    14041 tcttctacac tttgccggac caggacatct actatcggtt tctttcggcc atgaaggtat
    14101 tccctcaccg caacacccag gggatgtgca atatcgacta cgagcatgaa atggccatcg
    14161 tcggggtgat aggagacatc ggcaacgaaa cggtcctggc attgggacgc tacatcctgg
    14221 atcagaagac caacatggcc gaagtggatt ttgccgtacg ggcggagtgg cagcgcaggg
    14281 ggattgggac ctttctgctt cactacttgt gcgaaatcgc caagagcaag gggatttcgg
    14341 ggctgacggc ctacgtgctc gcggcaaacc ggtccatgtt ggcggtgttc cacaaggtcg
    14401 gatactgtat tcacagtcac tttgaagagg gcgttcacga gatctccttc cggttcgacg
    14461 agcccgccca ggcctgcatc accgagtgag gccccgggtt ttcgggaaag cattccggtc
    14521 gggcaaggcg gcccgaccct gccattccgg agctcatcca gaaaccggcc ttttttccca
    14581 tagaaggtct ttccctgcat gcatccgtcg ggtacccagc cagcaactct cgaggagggc
    14641 ttcggaccgg aattttgggg gagaattccg ccccaccagg tggccttcga cattgacggg
    14701 gtcgttgcgg ataccatggc ggtttttgtt cgcctggccc gggaacgcta cggtctgtct
    14761 catgtgacca aggcggacct tcgggtctat gacctctatc agtgcctgga cgtggaccgg
    14821 gaaatcatcc gggatttgat ctgccttacc ctggacgacc agcataccct cgaaaccccc
    14881 cccatgcccg gggcccggga ggtgctcacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn cgaccagcat accctcgaaa ccccccccat gcccggggcc cgggaggtgc
    15061 tcacggagat ttcccgggtt gcgcctctgc ggttcatcac ggcgcgcatc tggcccgagt
    15121 ccattatgga gtggctggat cgcctcctgc cccgggtggg accggggcaa atccgggtcg
    15181 tcgccacggg gtcccccgag gccaaacttc agattctcaa ggacctgggg gtacgatact
    15241 ttctcgagga tcgggtggaa acgtgtcggc tcctggcttc cgacgggatc caacccctcc
    15301 ttttcgacca gccctggaac cgggggccgg aagcctgcgg gcttccccgg atcgagagct
    15361 ggtcgcactt ccggcgaatg ctcttgctca ataacgggga gtgaagtaaa gtgccgggga
    15421 cgtgggtgga agagtacccg gaggcatttc ccgccaacga ccggaatccc atccggtccg
    15481 gaaccgcaac cgccccagga accaaacgag caaagatgag catgagttcc aacgaaccgt
    15541 tgccgatcca gatcaaggcc cgaaaggacg ggttcgtcct ggtgcccgac gccgaggcat
    15601 cctttgagac cattctcggg ttcctgcagc agcgcctcga agaggctcgg gactttttcc
    15661 atcatgctca aatgatcctg gatttgcggg aaaggcccct tcggaccgac gaaatccggt
    15721 ccctgcagtg tctgctcatg gacaaggttg gggcgagaat cacacagatt cgcctggacg
    15781 aggagacctc ctattttgcg tcgccgggag cgggcgggtc cgtccccaac ccgagatccg
    15841 tctccatgcc ggaaccggta ccggagtcga ccccgatgat cgttcacagc acctgccgat
    15901 ccggcacccg cgtggaatcg ggctccgatt gcgtcatcct gggggacgtg aacccggggg
    15961 cggaagtact cgccgttggg gacgtcatca ttttcggcaa cttgcggggg atcgcccatg
    16021 cgggggccgc tggaaaccgc cgggccagaa tctgggcctt gagcatcgaa cccagccaga
    16081 tccgtatcgc ggacctggtg gcggtgcccc ccaagggaaa caagccggtg cccaaacgct
    16141 acgaggttgc cgaaatccgg aaggacctca tcgaggtgat cacggtatga agacggatgg
    16201 gaccccggac cccgaacgct gacgttccgg ggcacgccgc aattccattc tcccaaacca
    16261 cttttcgaca accaacaact caggacggac ggttagaccc atggccggaa caactttagt
    16321 ggtgacttca ggcaaaggtg gggtcggaaa gaccaccacg gttgccaata tcggcactgc
    16381 cctggcccaa aagaaacaca gtgtggtgtt gatcgatgcg gacattggcc tgcgaaacct
    16441 ggatgtggtt atgggcgtgg aaaatcgcat tgtctataac ctcgtggatg tgattgaagg
    16501 caggtgcagg aaacagcagg ccatgatccg ggatcgcaaa ttcaacaacc tgtatctgat
    16561 tcccgccgcc cagacccggg acaaggactc ggtaaccccc gagcagatga aggacctctg
    16621 tacggagttg gccgaggagt tcgatttcgt cctcatcgat tgtcccgccg ggatcgagca
    16681 aggtttccga aacgccatcg ccggagcgca aaaggccctc attgtcacga cccccgaagt
    16741 ctccgccatt cgggacgcgg accgtgtcat cgggctcctg gaagcaagcc tcctcaggga
    16801 cattcatctc atcatcaacc gactgaatca tcgcatggtg aagaagggcg acatgatgtc
    16861 cactgcggat atcatctctc tattgtccgt cccgctcttg ggagtcattc ccgaaagtga
    16921 ggacgtggtg atttccacca accgaggggt ccctctggtc cacgagagaa acagtaaggc
    16981 aggaaaagcc ttgcagcgca ttgccgcgcg cctgaacgga gaggatgttc ccttggatga
    17041 cggtcaggga gacggcttca tggaccgggt aaagcggttc atttggagtt aacaaggagg
    17101 gtattttcat gcttagggca atccggcgct tcttcggcga gaaagcgagc ggtcaactgg
    17161 ctcgaaaacg tatgcaagtg gttctcatgc atgatcgatt ggacctgtca ccggagatcc
    17221 tggacgcgct aaagaacgac atcatcaccg tgctttcccg gtacatggaa atcgaccgaa
    17281 acaccattcg tgtggacctc gaacaaggaa aagactacgc ggccctggtg agcaacgtgc
    17341 acgtgaagcg ggtttttcga agggcggaat cggtttgaat ctccattgcg aggaactgct
    17401 tcatggcact ccaggtcagg ctgggcgcaa ccttgaggac ttatgtccac ccctacgacg
    17461 gttccgtcgg atatgagatg gtcgttcccc ccgggaccac cgtgagagaa ctggtgcgac
    17521 ggctgaaaat ccccgaagca gaagtgaggc tcatctttgt caacggcgtc agctccggtt
    17581 gggaaacggt cctggccggg gacgaacggg tgggcttgtt cccccccgta gggggcgggt
    17641 gagaaggagg ggaatccccc cggcgggctc gattcttccg gcctgcttgc ctgccgggga
    17701 gcaggccgcc caaatattcc gccaacctct tgaatttcga ttgcaaatac tccttttacc
    17761 ctctatttta ttttgcccag ggtatcctcc caagaataat tctgtttcga cacacaaaga
    17821 agccggcatg attgcctggg gcgatttatc tccccgaccg gactgaaacg gtgcccgtcc
    17881 cccctatccg gggggtgtcc gggcaattgg ggccaaggag cgatcgagat catgcgagcc
    17941 ctcgtggtgg aagaccataa ccagatgcgc atgatggtcc gcggaatgct cgaggaaacc
    18001 aaaaggttcg agctcgtgga cgaggccgcg gacggcgaac ggggatggga aaggatcctc
    18061 gacctgtgga ctcattcctc cgacgtctat gacctggtgg tttgcgatgt gaacatgccg
    18121 gtactcgggg gcctggaatt gctcaaacgc tgccggagtc gggcggaatt ccggtttctc
    18181 ccgtttatca tgatttccac ggccccttcc gagtccacca tcgcccaggc gttgggagaa
    18241 tacggggcca acgattttct cgtgaaacct ctctgctcca agttgttcga acaacgggtg
    18301 ctcctggttt tgtcccgggc tcagagtccc gaggaggtgc tttaccgccg ggccgaaagc
    18361 ctcaaggaaa gcgacgcagt ccgggaagct ctcgagctga tcgaatcctg ggaaaaggaa
    18421 aaccgcttct ccctggccaa atggttcaac ctgaaaggcg agtgcctctt caaggcggga
    18481 gaccccgaca aggcggccct cgagttccag aaggccactc aactgtgcga gatttacgtg
    18541 gcggcgtaca agaactacgc gtcggcccaa caaaagctgg ggaatacgga gaaagtcatc
    18601 gaggccctgg cccatatcga ggcgatcagc ccctcggacg actcccgtac cctgcttctg
    18661 ggggaattgc tcttcaaagc cggtcggccc gaggaaggaa aacggtatct cgagaacctt
    18721 ctcaagcgga gcaaccggaa cgaaaaagac aagattgtgc gcaaaacggc ccagttatac
    18781 ctggatgcgg ggctttacca ggaggccgaa ggcctcttca aaatgaccct gaaatccccc
    18841 ctggcggatg tggagaccta caatcaactg ggaattgccc tgcggcagca gggaaaattc
    18901 gaggaggcgg aacgctgcta cctgaccgcc ctcaacacgt atccgcgcca tgcggggatc
    18961 taccacaatt tgggggtcct ccacatggca agaagggact acagcaaagc caggcgaagt
    19021 ttccagaagg ccctcatctt caatcctcat ctccaggaaa gcaaagatat gattgaaaaa
    19081 atcgagctcc tggatcgaga gccgacctag ccttccagcc cccgcccccg ggacgccgat
    19141 cgaggccttc gggccctttg accgggccga accgcaagtc ccggaccgct cacgggatgc
    19201 ctccttgaat ccttccttga cactctccct tcttttgcgc tatgagtacc tgtgtcgatc
    19261 aatcataatc atcaggtgcc tcagggcttg atagggaatc ccgtgaaaat cgggaacggt
    19321 cccgccgctg tgaacgggga cgaaatcctc ggtggccact gtccaacatg aaggggacgg
    19381 gaaggcgagg agagtaggac gatccgtgag tcagaaaacc tgcctgatgg attcggggtg
    19441 acggcgcgtg gaaatgtcgg tcatccgaga aaggaccaag caaacttggg tgcaatcctt
    19501 agagttccgg ctctagggat ttttttttgg ggagcccctc ctccccgcac cggttactct
    19561 ttctgcgatt tccacaaacc tcttccaccc gggagccttc gaaactcccg ggtgcgccct
    19621 tttcctgccg acctttccgt ccggttgccg gatctatcgc tgcaagacaa cgatttcgcg
    19681 aaaccctttg ccaaagtgcg caattcgctt atgctatgga ggcacctcat agcgagcggc
    19741 cctcccggaa cagcgaacag gcttctaccg tcatcacggc gaaaacaggg cttcatcccc
    19801 tctttccgga ctccctcttc ctcactccga agccccggtt tccttgccgt ttcccatttt
    19861 ccgtcgttca cgagaggaac ttccccccct gttcccggat tctcctgtcc ctcgttcaag
    19921 tcctcacgag gagagcttca acccagggga agaaagttct caggatcggg caaccgcatg
    19981 ggggtttggg gtttcacggg ttcaagagag gcggggacaa tgaacggaag tccattggaa
    20041 tttcaaggtg tggctcagtg gctgggttat ttcttaggaa ctctttctgc agtggtgatt
    20101 cagggagaag gggcagcgta tttcgtgctc atgtttgagc ccatctcccg acgagggaaa
    20161 gtcatttacg atctcaatcc ggctcatcac ttggatttac gttccattcc tcttctcttt
    20221 ctggccggat ggacctggag caagaagtgc gtggtgaaac ccgactactt tccgaactcc
    20281 cgcatcagcg gtgccctgac gggcctggcc gggggtcttg cgatcctgct gatgtccgga
    20341 atcctcggca ctttgtacat gttcctgccc tttggggctt tggagaccgc catccgggcc
    20401 tgtgtcgtga tcgccatggc caatctcctg atccccatcc cccctttcgc tctcggccgt
    20461 gccctgtggt gccccttcca agataccttg aagcatcaaa aggcggtgga atgggccggg
    20521 gcaatcgccg tcacggcctt ggtgctcctg gaagtttgga tgaaaagccc cttcctgacg
    20581 gatcggatct tttccccggc atcctttctg agccgatgga ttctcagcgg ctagggctac
    20641 cctttaccca catctctgct atggcaggag agatgtctct tccccggggg tcaggtctcc
    20701 gtatggcttg tttcgtgcgt ccggagggtg taggggcgca atcccgccct tgttcacggg
    20761 acggttctgg gaccccacgg gtcggcttcc ctttgggacc tgttccgggg gtatcggcct
    20821 tgcggcctcg cccccctgct catggttgcg aaccctccgg gttcgctggg aggcggcgat
    20881 ctatccccgc ctgtcttccc cgggggtcag gtctccgtat ggcttgtttc gtgcgtccgg
    20941 agggtgtagg ggcgcaatcc cgcccttgtt cacgggacgg ttctgggacc ccacgggtcg
    21001 gcttcccttt gggacctgtt ccgggggtat cggccttgcg gcctcgcccc cctgctcatg
    21061 gttgcgaacc ctccgggttc gctgggaggc ggcgatctat ccccgcctga tcgcagatgt
    21121 ggggaaaggg caggactagg gatcgtcccc cttctttgcc ttttctttcc atgccgcgat
    21181 caccggaggg cggcaagaga gtgtgtctcc cttccctccc cggcggctgg gaaaggcccg
    21241 gggtctcccc acggattcgc catggcaagc cgcggggccc gatctacctc acttcgtcgt
    21301 agatgagtcc tcccacggcc cctgcggcgg ctcccacggc accgccgacg atgaggggtg
    21361 cgcccacgat ggcggccgtc ccgactcccg ctgcggttcc gatggccccg ccgctgagga
    21421 accgctgctc tctttggctc attcccgcgc aacccgtcat cgaggcgaca agcaagataa
    21481 cgaccacgat tctgccccag tccgtccttc tatggtttcc atccacttct tcctgaaacc
    21541 ccaggcatcc agttccgtcc gcgtcattgc gtgcccgtgg gcacggccgg atttcggtca
    21601 cgactcaacg agtttgtttg tcacattacc ccataatcgg ttaacgtccg aatggaaagg
    21661 aacgatgaac ctccgccgca aggccgggaa cgaacactct tgttcggctt ggagtcgtgg
    21721 tacactgcgc gggtgggtac cccctcgagt tccctttcgc ggtccggatc atgtccggca
    21781 gggaggtctt tgccgtgctc gttcacccct tccctctcac ccattgtttg gccccttcgg
    21841 ccggaaagga agtgtttcat ggaatttgac gacttgatcc ccgaccatca actggatcat
    21901 gtgggcctgt accagctcag gcacgtttac tggaaccgct cgaccccgga gctctacgag
    21961 ctcgccattc gacgctacga aggacagatc gcccacctgg gccccctggt cgtgtccatg
    22021 ggccagcaca cgggacgggc cgccaaggac aagtacgtgg tggacgaacc caccacgacc
    22081 gacgagatct ggtggggcaa agtcaacgtg aaatatcccg aagagcggtt ccacaccctg
    22141 cacaagcgca tgaccaacta cttgcgggga aagacggtct ttgtgcagga ctgctacgcg
    22201 ggagccgacg aaaggcaccg tcaatcggtc cgagtggtca cggaatttgc ctggcacagc
    22261 cttttcgccc gcaacatgtt catccaactg cctcgggacg tggctcaaat caagagcttc
    22321 gttccggatt tcaccgtcat ccattgcccg aacttccacg cggaccccga agacgacgag
    22381 acccgcagcg gcaccttcgt ggccctgcac ctgagcaaga agctggtgct gatcggcggg
    22441 acggcctacg ccggggaaat caagaagtcg atcttcacgg ccctcaactt tctgctgccc
    22501 gcccaggatg tgctctccat gcactgttcg gccaacgttc acaaggacaa cccggaggac
    22561 gtggcggttt tcttcggcct ttcgggaacg gggaaaacca ccctttcggc ggaccccaaa
    22621 cgattgctca tcggggacga cgaacacggc tggtcggaaa acgggatctt caacttcgaa
    22681 ggaggctgtt acgccaaggt gatcaatctc tccaagtctt cggagccgga aatctacgaa
    22741 tgcacccgcc ggttcggaac catcctcgag aacgtctcca tgagcccggt catccgtcga
    22801 ctggacctgg acgacgcccg gctgacggaa aacacccggg cctcgtatcc cttgtcccac
    22861 cttcccaaca tcgtgccttc gaggaccgcg gggcatcccc gaacggtcgt tatgctgacc
    22921 gccgacgcct tcggcgtact tcctcccatc tcccggctga cggaggaaca ggccatgtat
    22981 catttcatca gcggctacac ggccaaggtg gccgggacgg aaaagggcgt caaggaaccc
    23041 ggcgccacgt tcagcgcctg tttcggagcg cctttcatgg ttcgtcatcc ctcggtttat
    23101 gcccagatgc tggccaagaa gatccgggat cacaaggtcg gttgctggct ggtgaatacc
    23161 gggtggacgg ggggacccta cggagtcggc tcccgcatga agatcgagtt gacccgggcc
    23221 ttgctcaacg cggccctggc cggagacctg aaccacgtgc ccatgaagcc ccatcccgtc
    23281 ttcggggtcc tggtgcccac ggaaagcccg ggggttccct cggagctgct ggacacccgc
    23341 cgcacctggt ccgacccgga acgatacgat gcccaggctc gacgactggc ggtcatgttc
    23401 cgggaaaact tcgaacagtt caaggaccag gcatcccccg aagtccgggc ggcgggacca
    23461 cgggccgatt gaggggaggg gttcgcagcc gaaaccgcgg caagacgaaa gcaggagcca
    23521 cccatccgcc ggtagaggac ttgcacctcc aagggaacgc gccccgccgg gggcaaccca
    23581 aaagcaaaag gggtcggccc gtattgggcc aaccccttga ttttcctgga ggcggcaacc
    23641 agactcgaac tggtgaataa cggttttgca gaccgctccc
//
LOCUS       DAOOVC010000053        23006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1516_length_23006_cov_6.744891, whole genome shotgun sequence.
ACCESSION   DAOOVC010000053 DAOOVC010000000
VERSION     DAOOVC010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 23006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23006
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1516_length_23006_cov_6.744891"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..671
                     /locus_tag="PLM79_08770"
     CDS             102..671
                     /locus_tag="PLM79_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86442.1"
                     /translation="MKKGIPRTLPPLGLIEMGRLGEVAVRVVAANLQGVLGIPVDLLQ
                     PMDIPTESFQEHRKQHDAGLILKHLASRSFPNHLGVLALTTVDLCSPILTYVYGEAEV
                     GGTTAVVSSHRLRSNEDGSIVSLGRYYERLAKVSLHEVAHILSMFHCEKDDCLMHYCS
                     KTHHLDRLGIAFCRRCEYVLKKNLALRCS"
     gene            889..1344
                     /locus_tag="PLM79_08775"
     CDS             889..1344
                     /locus_tag="PLM79_08775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV86443.1"
                     /translation="MEDVGPRSGKSRVRRAVTADLDSILDIEEGSFPSPWNRDYFQAA
                     LKDLFLVFEAESVQGFLVACAYQAAKKALILKVAVHPEHRGKGIASELLRTAVELLKT
                     ENLEEVELDVDVFKSGAMRLYERFGFRVVTCVSTDPEENDAFCVMKRRL"
     gene            complement(1426..4053)
                     /locus_tag="PLM79_08780"
     CDS             complement(1426..4053)
                     /locus_tag="PLM79_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOV86444.1"
                     /translation="MVWTHFLRALPVLGVMAGSFLFPPEIYGQTPVPGSGERTEAPVV
                     SPQALPQVLEEGRTARKRELSAVRARTTQAKEAYLEAKRDLQSVTVALASLKTTLFIR
                     EMSLAQIQEQIQSLSARRSQVLKIQSETARAVESMKQEQAALQESRNVLEKQMESLRS
                     TLDPVGWSREVEESFSAYLRTLKAVQHASNRLIEYAAKRLQTLGREVQMLEEALAELR
                     AREEVRKAEFLKSESVLTAKEQVGGIWQSLETMTHRGWQWLSTLILSGALFGFVQSKF
                     PLLLGLCISALLLRWGAGKIRSVALPWLVSREAATGDVFLRIFLRMVQIAVRYLFLVG
                     LTLLLGLGLWGLQLLPSAPARLALATVGSFVALRLLFRFFHALFAGGREEGILALGPG
                     IGRHYSRGFKLLAAYGLLGSLALFTAQVLDFPEPTRHFLRYLFEAGLLALPLWIFRSP
                     HLQRLLSDLYGEKGEPWSRSARSVWGLVLFLLVAAILSDLLSFHGLSVYLVHSTLISL
                     GGVLLVMLLRVGLDESARYLWHPRDGYLARRYPDGGEWLTRLHEWSGKTISGLTWVTA
                     LVGVLVAWGITPGTVVQVLKGLTWGIDVGPVKVTPLNAGLLILVFYGTRWISRLVRSF
                     LQTRVFPRTGWDLGIRYTITTIVHYVTVVLGILVGLNVLGFPLANLALILGALGVGVG
                     LGLQDIVGNFVSGLVLLFERPIKVGDVLVIDGQWGEVKQIRMRSTVFQTADRCTLIIP
                     NSQLTSQRVLNWTHEGRGPNRLQLKVGVSYDSDVKRVTRLLTEICKGNPRVMDEPGPQ
                     VFFQAFGESSLDFNVWVFVRSPGDRIPATHEINQAIYDCFREEGIQIPFPQRDLHIHD
                     GNEGIKGKQAHGSSSASDD"
     gene            complement(4062..5555)
                     /locus_tag="PLM79_08785"
     CDS             complement(4062..5555)
                     /locus_tag="PLM79_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOV86445.1"
                     /translation="MKTRFLGILFLSGLYVASGVGAAQSPNSAATEECLGCHAGLHPG
                     IVEGWKKSRHAATTPGQAAAVEGLALKVTSRAIPEALKGVTVGCAECHTLRADAHQGS
                     FDHNGANIHVVVSPKDCATCHTEEAQQYDRNLMAHAYGNLVDNPVYGMLMQAINNTPV
                     FTKGKLTTEPANDLTKADSCLYCHGTKLEVKGTVTRDTEMGEMTFPILSGWPNEGSGR
                     INLDGSKGSCSACHSRHEFSLEMARKPYTCKECHVGPDVPAYKVYEGSKHGNIFSTKQ
                     SSWNFKEVPWTLGKDFSAPTCAACHMSLLVNTEGKVVVRRSHEVKNRLPWRIFGIIYA
                     HPHPREANLTPIRNKANLPLPTDFDGSFADPYLWNEQERKAAAGQMQAACLACHHQSW
                     VDGFWLRFDNTLETTNRTILAATQILGEIWKRDLALGLEKKGNPFDEYIERVWSNGWL
                     FYANSVRFSTAMGGGGDYGVFADGRYHLMKTVREMEDWLELRKGRRK"
     gene            complement(5671..6579)
                     /locus_tag="PLM79_08790"
     CDS             complement(5671..6579)
                     /locus_tag="PLM79_08790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOV86446.1"
                     /translation="MDVVAAAFFWAVSGSSGKYLFHQGLTPFQVVQLRVTLAALFLFL
                     FLAVFRRDLLPIRGRDLPYFVGLGIGGMAMVSFTYFFTISRIQVAAAILLQYLAPAII
                     AVYAVVFWHERLTRPTITALVGSTLGCYFAVGAYNLEFLTLNWLGLVSGIGSGIAYAW
                     YAVQAEKGMRRYHPWTVLFYALLLAALFWNLALPPFQAFGGNYDPLEWFWILYIALLG
                     TVVPYGLYTQGINLIRSTRAGITATLEPILAAVVSYFFLGEVPAPLQVMGGAMVIGSI
                     ILLQLRQESDERSPDRIRSRGPFPNP"
     gene            complement(6632..7414)
                     /locus_tag="PLM79_08795"
     CDS             complement(6632..7414)
                     /locus_tag="PLM79_08795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV86447.1"
                     /translation="MHFRIIDAHAHCGIQDLYPPQSLEDYLDHVRGSEIRGAVFIPPV
                     FEIYDRYDPYFEDNAQWVRRRQSANSYLLSLGGTRFEVFPFFFIWNDFAVEEISEQHK
                     GIKWHRHPDEPVYHYDDPRCSSAIREIRRRNLPVCLEEEFGHTLRFIRDLAPGMSIII
                     PHCGLLNGGYEGFVRAGIWELPNIHTDTSLVPSSIVGDYVQRYGHSRIMFGSDFPFGD
                     PVSEMRKIQRMGFPEDVKQAILGQNVIRLLGESPGEGGPDPS"
     gene            complement(7491..8135)
                     /locus_tag="PLM79_08800"
     CDS             complement(7491..8135)
                     /locus_tag="PLM79_08800"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HOV86448.1"
                     /translation="MKSEKKARTQTPARLLETAGELFARHGYRAVTVREICRRARVNI
                     AAIHYHFGDKFGLYSAAVRNLVSRESSTPPPQGGAADGSTPEEQLYAFVRSLLFRTLR
                     EGNTPWSGKILLRELLEPSPAFPELFQQAILPLLEQARFLLGQLAPPNLPEETLRLCA
                     LGLLGQCLFYQHAPEHLLDPASGKSKSSRMEAVADHMTRFFVHAVKGFAKEEFL"
     gene            8545..8760
                     /locus_tag="PLM79_08805"
     CDS             8545..8760
                     /locus_tag="PLM79_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86449.1"
                     /translation="MKFQCIRCHSSWGEGIPEEEGYSHGLCAPCLKEALTPLYRKRQI
                     SEGNFDCFGRSQGYCDQSACKYREICL"
     gene            9098..9328
                     /locus_tag="PLM79_08810"
     CDS             9098..9328
                     /locus_tag="PLM79_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86450.1"
                     /translation="MFTTRELEIFARIRELGQRARRLKQALEHAGAARGPEGRTREDL
                     LDELEGLRRERKRAESERIDARAERMRLLGHE"
     regulatory      9449..9545
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9652..10932
                     /gene="thiC"
                     /locus_tag="PLM79_08815"
     CDS             9652..10932
                     /gene="thiC"
                     /locus_tag="PLM79_08815"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOV86451.1"
                     /translation="MRTQLDYAKAGIVTEEMRQAAAVESVSPEELRDLIGQGLAVLPK
                     NVGHSFPILRAIGKNLKTKVNANLGTSGECASREMESAKLKAALDAQTDSIMDLSTGG
                     DLAGFRHFFLENSPVMVGAVPIYSVATEMVAKHEPLDTMDPDRLLRSIEKQCAEGLDY
                     ITVHCGVTLASVKKLETFPRIMPCVSRGGSIHMHWMKKNGKENPLYEYYDDLLEIAHR
                     HDVTLSLGDGFRPGTILDAIDGAQIEELMVLGELARRAWQRGVQVMIEGPGHVPLQEI
                     QSHIQLQKRLCRGAPFYVLGPLPTDIAAGYDHITSAIGGALAGAAGADFLCYVTPAEH
                     LGLPVEEDVYLGVMAARIAGHVADIVKGVPGAVERDRRISIARQNLDWEGVIRESLDP
                     ELVKRRLQITQDREACSMCGKLCAVKISRQVQNV"
     gene            11120..13048
                     /locus_tag="PLM79_08820"
     CDS             11120..13048
                     /locus_tag="PLM79_08820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86452.1"
                     /translation="MGRPTYKELERRIQKLEQLVNLKVYPQEVHQEGRPRIYFRMGAD
                     WTFDFFDKGISDLTGYDLEDFLDRQVYWLDLIHEEDRDPLLDSIRVAMESDRYCLAEF
                     RIRCRTGQTRWMRMSGPIFCDEDGRLQHIQGILCDITAQKSMEVTLDSEHEVFVWVAN
                     SLEDGIYIVSDDYRIQFMNKALVELMGNHVGRICHEALFHRPSPCPRSPSDSLHHEGC
                     GIHQYYMGELGRTFEVRSFPIKTRYGTGKLGKFKDITRTKMLKHKVKHLAVRHQAILD
                     AANLADIGIFRLETHEGIEARFRCANIAFCRITGYAAEELLQMSFLDLIPEAFREEVL
                     DLYRRRQRGEIPNESYERKMVRKDGSLITVNVSVAAIQTTSGRVATIGFLQDVTEREK
                     YQKSLWLSQRLSSIGRVAAEVAHEINNPLTSVMTFNKLVERIIQQEPFPVQRTAELRD
                     YTRYLNAEASRCAEIARNLLEFSRHNEIEIKKEHHIHEILEKTLNILRHRAALTDIRI
                     LTSYAEDVPPMLCDFKRLQQAFVNILWNGIEAMPEGGVLSVSTNYDAQERLVSVVIGD
                     TGVGVPEEDLDNIFEPFYTTKAEGKGVGLGLSVAYGIIRQHQGRIHVQSMVGKGTQFT
                     ILLPADALSVSEVAECGE"
     gene            13312..14658
                     /locus_tag="PLM79_08825"
     CDS             13312..14658
                     /locus_tag="PLM79_08825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86453.1"
                     /translation="MKEDARILVVDDEPMVCLSLVNWLKEENYLAQSAEDGPSAIAAV
                     SQEPWDVVLLDLKMPGMDGLEVLRQVKKISPQTVVIMMTAYASIASSVQAMREGAYDY
                     IIKPLDVQELTLMLKKIVDHQRLVNENILLRKKLTERYAYENIIGKSNAMQKVFEMIR
                     TVADTSATVFISGETGTGKELVARAIHTNSSRKYNPFIAVSCGALPETLLESELFGYE
                     KGAFTGADRMKKGRFELAHGGTLFLDEIGDISMRTQVKLLRVLQEKKFERLGGTESIE
                     VDVRLITASNRDLPKAIEAGDFRSDLYYRLNVVNIHLPPLRQRTEDIPLLIEHFMRKF
                     NLEFNKKFTRVEDAALNLMMEYRWPGNVRELENVIERAIVIDNGPEIKVAQLPFFKKD
                     EACAEGGPLTLEEMERTHIQKMLDLCKGNVSKAARTLSIDRTTLHKKIKQYGLSKR"
     gene            14668..15231
                     /locus_tag="PLM79_08830"
     CDS             14668..15231
                     /locus_tag="PLM79_08830"
                     /inference="COORDINATES: protein motif:HMM:NF019610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86454.1"
                     /translation="MIDQSPAIHLVILEAEEPDYLRQVAESIQEQFDLDVTVGKPLGL
                     PTYALDPVRKQYNSNLILKRLAETCPPHAVKVLGLTAVDLFSPIFTYVFGEALFQGKS
                     AVISAFRLRGVPDAKAALGCPPLMNRMEKEAVHELGHTFGLRHCLDPDCVMNYSSGVQ
                     CADRKFPFFCPACRELLLWSLKKSMRE"
     gene            15560..16501
                     /locus_tag="PLM79_08835"
     CDS             15560..16501
                     /locus_tag="PLM79_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86455.1"
                     /translation="MKEKTKEKSKVTAFKTVPSGEEKCIWMEAGVLDYKLCNNFYNCH
                     TCAFDKAMKETAERNLLAKVERMEPPGKKSNIVTWQESMRKRGGLERKCRHYLTGRAP
                     FRLCPYDFECHACGFDQMLEDGLELQLPYPVLQMPLIDGYYVPEGHFFHLGHAWARVE
                     QGGRVRIGLDDFSMRVFGTPEKLELPLTGERVRFSDVGLAFKRMGKEASVLAPLSGIV
                     SAVNAQAAKEPDLVRHEPYNKGWLMVIEPTDMKKNLKELLYGKQTTEWIRSEHQSLVE
                     MVSEVGITFADGGQIEDVFGNVPNLAWEKLARRFLRT"
     gene            complement(16676..16918)
                     /locus_tag="PLM79_08840"
     CDS             complement(16676..16918)
                     /locus_tag="PLM79_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86456.1"
                     /translation="MDFAEFFQHCRNETTVSDFESYLSYCIFQKKTARDECTLEMYSY
                     IRDRAFEQRDEQAARVLYELASAADWKEGDWEGALR"
     gene            17925..20951
                     /locus_tag="PLM79_08845"
     CDS             17925..20951
                     /locus_tag="PLM79_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOV86457.1"
                     /translation="MVVGGGVAGIQAALDLANTGFYVYLVEKSPAIGGVMAQLDKTFP
                     TNDCSMCILSPKLVEAGRHLNIRLMTRAEVQELTGEVGRFTATVRERARYIDMEKCIA
                     CGVCAEKCPKKVEDEFNEGLSQRKAAFVKYPQAVPLKYSIDEETCIYFAKKGKCKACE
                     KFCPAGAVDFAQEDKIHRLEVGAVILAPGFRTFRPEAYDYGYGTYANVVTGTEFERVL
                     SASGPFEGRLVRPSDGKAPKKIAWLQCVGSRDTQRGAHGYCSGVCCMYAIKEAVIARE
                     HAHGDLDTAIFFMDMRTYGKDFERYYDRAREESGVRFIRSRVHSIEPVAPGSDDLAIS
                     YVLDDGVPRTEVFDMVVLSVGIETPRDVQDLAARLGVEMNVDGFAETASFAPVATSRP
                     GVFACGVATGPKDIPYSVMEASAASAASAAALSSSRHTLTRKKTYPEELPVAEGEPRI
                     GVFVCHCGINIASVVDVPAVREYAGTLPNVVYVANNLFTCSQDTQKLIREAIEEHRLN
                     RVVVAACTPRTHEPLFQETIREAGLNRYLFELANIRDQDSWVHQAEPEKATEKAKDLV
                     RMAVAKVALLEPIERIEVDLTHSALVVGGGVAGMTSALNLADQGFGVVLVETKEFLGG
                     HALKVPRTWKGEDVASHVEDLVRRVTNHDRIEVITGAKVTKAGGFVGNFFSTVETPQG
                     EKEIRHGVVILATGAHSLKPDEYLYGQSDRVTRWHELDKLFREEPRRLEEAEAVAFIQ
                     CVGSREPERPYCSKICCTASVQQAIELKTRKPDLDVFILYRDLRTYGPREELYRRARE
                     LGVLFLRYSLDAKPLVEPVTVNGREKIRITVQDHVLQRPVHLEADYLNLATAIIPKDL
                     ESLAQFYKVPLNQDGFFLEAHMKLRPVDFATDGVFVAGLAHYPKPMEESVAQAQAAAS
                     RAATVLTQAKVEVEPIVSWVDQEVCIGCGLCEVSCPFSAIRLTKVHGKGYRAENISAS
                     CKGCGICAASCPQKAIDMKHFRDRQIIAAIQAGGQG"
     gene            21052..21447
                     /locus_tag="PLM79_08850"
                     /pseudo
     CDS             21052..21447
                     /locus_tag="PLM79_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
     gene            21911..>23006
                     /locus_tag="PLM79_08855"
     CDS             21911..>23006
                     /locus_tag="PLM79_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86458.1"
                     /translation="MANEQNAKKIGAVMVVGAGVAGMQASLDLAESGYYVHLVDKSPT
                     IGGIMAQLDKTFPTNDCAMCIISPKLVEVGRHLNINILPNTEVESVEGEAGNFKVTLQ
                     CQPRYIDLEACTACGQCRLVCPATAVNEFNQKLDVREATYMRYPQAIPRGFMIDRAVC
                     IGCGLCEKVCLAEAVHYDDTVRKTEIEVGAVVLAPGNEVFDPSQFDTYNYLGFPNVVT
                     SLEFERILSASGPYRGHLMRPYDREEPAKIAWLQCVGSRNINACDNSYCSAVCCMYAI
                     KEAVIAKEHAHGDLDTAIFYMDMRTYGKEFEQYYNRAQQHGVRFIRSRVHSVEPEGEC
                     DLRITYVDEAGEQKSEVFDMVVLSVGFEVGL"
BASE COUNT         4950 a         6851 c         6997 g         4208 t
ORIGIN      
        1 tgagcaggat caccctgcac aacaagatca agaaatacgg gctgacgccc cccgtcccat
       61 gacccccaaa tcccggggag acccgaaccc ggaaaggatg tatgaaaaaa ggcattcccc
      121 gaaccctccc tcccctgggc ctcatcgaga tgggccgatt gggagaagtc gccgtacggg
      181 tggtagccgc caacctgcag ggtgtcttgg gaatccccgt ggatctcctg caacccatgg
      241 acattccaac ggagtcgttt caagagcaca gaaagcagca cgacgcggga ttgatcctca
      301 aacaccttgc aagcagatcc tttccaaatc acctgggggt cctggccctc accactgtgg
      361 acttgtgcag ccccatcctt acctacgtgt atggggaagc agaggtgggg ggaaccaccg
      421 ccgttgtttc cagccatcgc ctgcgcagca atgaagacgg cagcatcgtg tccctgggcc
      481 ggtactacga acgactggcc aaggtgtctc tccacgaagt ggcccacatc ctctccatgt
      541 ttcactgcga aaaagacgac tgcctcatgc actattgttc caagacccac cacctggacc
      601 gattggggat cgccttctgc cgacgatgtg agtacgtcct gaaaaagaac ctggctctgc
      661 gctgctcctg accccgatcg agggatcctg cgggggaagc tctctcacgc accgaacccc
      721 ggcggggagt cgcggcaacc tcgaattctt cgggcaggtc cgaccgtaag gaatgacgaa
      781 gggcaaagga cccggcggaa atcggaccgg gaagggactc gaagatccgc ggtctcgggt
      841 cttgatcccc catcgctccc tttgctttgg gtcaaggagg tgtgagagat ggaagatgtg
      901 ggccctcgat ccggaaaaag ccgtgttcgg cgagcggtta cggcggatct ggattcgatt
      961 ctggacatcg aggaggggag ctttcccagt ccctggaacc gggattactt ccaggcggcg
     1021 ttgaaggacc ttttcctggt cttcgaggcc gagagcgttc aggggtttct tgtcgcgtgc
     1081 gcataccagg ccgcgaaaaa agctctcatc ctgaaagttg ccgtgcatcc cgaacatcgc
     1141 ggcaaaggca ttgcctcgga gttgctccgc acggccgtgg aattgctcaa gaccgaaaac
     1201 ctggaagaag tggagctgga cgtggacgtg ttcaagagcg gtgcgatgag gctctacgaa
     1261 cgattcgggt tccgggtggt gacttgtgtt tccacggacc cggaagagaa cgacgccttt
     1321 tgtgtcatga agcggagact ctaggagaac ttcagggcgt cttctcccgg gtcgcccggc
     1381 ggtgcggtgg gaaaatccga ccttagagcg acaacccggg atgcttcagt cgtcggatgc
     1441 gctcgatgaa ccgtgcgcct gctttccctt gatgccctcg ttcccgtcgt ggatatgaag
     1501 atcccgctga gggaacggaa tctggatgcc ctcttcccgg aagcagtcgt agatcgcctg
     1561 gttgatctca tgggtggccg ggatgcggtc cccgggagat cgaacgaaaa cccatacgtt
     1621 gaaatccagg gagctttctc cgaaagcctg gaaaaaaacc tggggtccag gttcgtccat
     1681 gacccgggga ttgcctttgc agatctccgt caagagccga gtgacccgct tgacgtccga
     1741 gtcgtaggat acccccacct tgagctgaag gcggttgggt ccccgccctt catgggtcca
     1801 gttgagcacc ctctgggatg tgagctggga gttgggaatg atcagcgtgc agcggtccgc
     1861 cgtttgaaaa acggtgctcc gcatgcgaat ctgctttacc tccccccatt ggccgtcgat
     1921 gaccagcacg tcccccacct tgatgggcct ctcgaacaag agaaccaacc cgctcacgaa
     1981 attgcccaca atgtcctgga gaccgagtcc cactccaacg cccagggccc caaggattag
     2041 agcgagattg gccaggggaa atcccaggac attcaacccc acgagaatgc ccaggaccac
     2101 ggtgacataa tgaacgatgg tggtgatggt gtatcgaatt cccaggtccc acccggtccg
     2161 gggaaacacc ctcgtctgaa gaaagctccg caccagccgg gaaatccacc gagtcccgta
     2221 aaagacgaga atcagcagtc ccgcgttaag gggcgtcacc ttgacgggcc cgacatcgat
     2281 cccccaggtc aggcctttga ggacctggac gaccgttccg ggagtgatcc cccaggccac
     2341 cagcaccccg accagggccg ttacccaggt aaggccggag atcgtcttcc ccgaccattc
     2401 gtggagccgc gtcaaccatt ctccgccatc gggatagcgg cgggccaggt aaccgtccct
     2461 ggggtgccac aggtatcgcg ccgattcgtc cagccccacc cgcaggagca tcaccaggag
     2521 aactcccccc agggagatga gggtggaatg gaccaggtag acggaaagcc cgtggaagga
     2581 aagcaaatcc gacaggatgg cggccaccag gagaaagagg accagtcccc acacgcttcg
     2641 ggcggaccga ctccacggtt ctcccttttc cccgtacaaa tccgagagga gcctctggag
     2701 atggggactt cgaaagatcc acaggggcag ggccaacaac cctgcttcga ataggtaccg
     2761 caggaaatgt cgggtcggct cgggaaaatc gaggacttgg gccgtgaaga gggccaggga
     2821 ccccaggagg ccgtaggctg ccaggagctt gaatccccgg gagtagtgcc ggccaatgcc
     2881 gggtcccagg gccaggattc cctcctcccg gcccccagcg aacagggcat gaaagaagcg
     2941 gaacaagagc cgcagggcca cgaaactgcc cactgtcgcc aaggccagtc gcgccggagc
     3001 cgagggcaga agctgaaggc cccacaggcc aaggcccaaa agcaaggtga gaccaaccag
     3061 gaaaagatag cggacggcga tctggaccat tcgtaagaag attcgcagaa acacatcccc
     3121 ggttgctgct tcccgcgaca ccagccacgg cagcgccacg gaccggatct tcccggctcc
     3181 ccaccggagc agcagggcgg aaatacagag ccccagcagc agcgggaact tgctttggac
     3241 aaaaccgaaa agggctcccg agaggatcag agtgctcagc cactgccaac ctcgatgggt
     3301 catggtttcc agggattgcc aaatccctcc cacctgctcc ttggccgtaa ggacggattc
     3361 gctcttgaga aactccgcct ttcgaacctc ttcccgggct cgaagctcgg ccagggcctc
     3421 ctccagcatc tgcacctcac gtcccagggt ttgcaggcgc ttggcggcat actcgatcag
     3481 tcggttcgag gcatgctgca ccgccttcaa ggtacgcagg taagccgaga aggattcctc
     3541 gacctcccgg gaccacccga ccggatccag ggtgctccga aggctttcca tctgcttttc
     3601 caggacgttc cgggactcct gaagggcggc ctgctcctgc ttcatggact ccacggcccg
     3661 ggccgtttcg gactggatct tgaggacctg ggatctccgg gccgagaggg actgaatctg
     3721 ttcctgaatc tgggccaggg acatttcccg gatgaagagg gttgtcttca aggaagccaa
     3781 ggcgaccgtc accgactgca aatccctctt ggcttccagg taagcttcct tggcctgggt
     3841 agtgcgggcc cgcaccgccg agagttctct ttttcgggcc gtcctcccct cctcgaggac
     3901 ctggggaagg gcctgggggg aaacgacggg agcttccgtc ctctccccgg aaccgggaac
     3961 gggtgtctgg ccgtagattt ccgggggaaa aagaaagctt cctgccatca cccccagcac
     4021 cggcagagcc cgaaggaaat gggtccaaac catgatgtcc tctacttcct tcttcctttg
     4081 cggagctcca gccagtcttc catctcccgc accgttttca tcaggtgata tcgcccgtcg
     4141 gcgaacaccc cgtaatctcc gcctcccccc atggccgttg aaaaccgcac ggaattggcg
     4201 tagaagagcc atccgttgct ccacaccctc tcgatgtatt cgtcgaaggg atttcccttc
     4261 ttttcaaggc cgagggccag gtcccgcttc cagatttcgc ccaggatctg ggttgcggcc
     4321 aggatggtgc ggttggtggt ttccagggta ttgtcgaaac gaagccagaa gccgtccacc
     4381 caggattggt gatgacaggc caggcaggcg gcctgcatct gcccggcggc cgccttccgt
     4441 tcctgctcgt tccacaggta tgggtcggca aaagacccgt cgaaatccgt gggcagggga
     4501 agattggcct tgttccgtat gggcgtaaga ttcgcctccc ggggatgggg atgggcgtag
     4561 atgatgccga agatgcgcca cggaagacgg ttcttgacct cgtgagatct tcgaaccacc
     4621 accttcccct cggtattcac cagcaggctc atgtggcagg cggcacacgt gggagcggag
     4681 aaatcctttc cgagggtcca gggaacctcc ttgaagttcc aggaggactg tttcgtggaa
     4741 aagatatttc cgtgtttgct cccttcgtag actttatagg ccggaacatc cgggcccacg
     4801 tggcattctt tgcaggtata gggtttgcgg gccatctcca gggagaattc atgcctcgag
     4861 tggcaagcgg agcaagaacc cttgcttccg tccaggttga ttcgcccgga tccctcgttg
     4921 ggccaccccg aaaggatggg aaacgtcatc tcgcccatct cggtgtcccg ggtgacggtg
     4981 ccttttactt cgagcttcgt cccatgacag tataggcatg agtcggcctt ggtcagatcg
     5041 ttggcgggct ccgtggtcaa tttgcccttg gtgaaaacgg gggtgttgtt gatggcctgc
     5101 atgagcatcc cgtacacggg gttgtccacc aggttgccat aggcgtgggc catgaggttc
     5161 cggtcgtatt gttgggcttc ttcggtatgg caggtggcac aatccttggg actcaccacc
     5221 acgtgaatgt tggcgccgtt atgatcgaag ctgccttggt gagcgtcggc acgaagggtg
     5281 tggcactcgg cgcatcccac cgtcacccct tttagggcct cggggattgc ccgactcgta
     5341 accttcaggg ccaatccctc caccgctgcg gcctggccgg gagttgtggc cgcatggcgg
     5401 ctctttttcc agccttccac gatgccggga tgaagtccgg cgtggcaccc caggcactcc
     5461 tcggtggccg cgctgttggg cgactgcgcc gcaccgaccc cggaggcaac atagagaccg
     5521 gaaagaaaaa ggatccccag aaaccgtgtc ttcatactcg ttcacctccg atttgggtgg
     5581 aagtcaatgg aacacggaag gcatctctgg acggagacat aatgccgatt cccgtggaaa
     5641 atgtccacgg agattccatg aaagggaagc tcaagggttt gggaaaggcc cccgggagcg
     5701 aatccgatcg gggctcctct cgtcggattc ctgccgcagc tgcaaaagga tgatggaacc
     5761 gatcaccatg gctcccccca tgacttggag gggtgccggg acctcgccca ggaaaaagta
     5821 ggaaaccacc gccgccagga tgggctcgag ggtggcggtg atgccggccc gggtggatcg
     5881 aatgaggttg atcccctggg tgtagagccc gtaggggacc acggttccca ggagggcgat
     5941 gtagagaatc cagaaccact cgagaggatc gtagttcccc ccgaaggcct ggaagggagg
     6001 gagcgccagg ttccaaaaga gggcggccag gaggagggcg taaaagagaa ccgtccaagg
     6061 atggtagcgc cgcatcccct tttccgcctg gacggcatac caggcgtagg cgatgccgga
     6121 accgattccg gaaaccagcc ccagccaatt cagggtcagg aactcgaggt tgtaggcccc
     6181 tacggcaaag taacaaccca gggtggaacc caccagggcc gtgatcgtcg ggcgggtcaa
     6241 acgttcgtgc caaaagacca cggcgtaaac ggcaatgatg gcaggtgcca ggtattgcag
     6301 gagaatggcc gccgccacct ggatcctgct aatggtaaaa aagtaggtga aggacaccat
     6361 ggccattccc ccgattccca aaccgacaaa atagggaagg tcccgtccgc ggatgggaag
     6421 caggtcccgg cgaaagaccg ccagaaagag aaacaggaac aaagcggcca gggttacccg
     6481 cagttggacc acctggaagg gggtcaaccc ctggtgaaag aggtacttgc ccgaggaacc
     6541 ggaaacggcc cagaagaagg ctgcggcaac aacatccagg tatccccgtc gggggatgcc
     6601 gacgaaattg gatttccggg ggtccggggg ttcatgaagg atccgggccg ccttcccctg
     6661 gggattctcc cagcaagcgg atcacgtttt ggcccaggat ggcctgcttc acgtcctcgg
     6721 gaaaccccat ccgctggatc ttcctcattt cggaaacggg gtccccaaag ggaaaatcgg
     6781 agccgaacat gatccgggaa tggccgtagc gttggacgta atcccccacg atggacgagg
     6841 gaaccaggga ggtgtccgtg tgaatgttgg gaagctccca gatccccgct ctcacgaagc
     6901 cttcatagcc gccgttgagc aggccgcaat gaggaatgat gatggacatc cccggcgcca
     6961 ggtcccggat aaatctcagg gtgtgaccga attcctcctc caggcagacg ggaaggtttc
     7021 ttcttcggat ctcccggatg gcggaagagc accgggggtc atcgtaatgg tagacgggct
     7081 cgtcgggatg gcgatgccac ttgatgccct tgtgttgctc ggaaatctct tccaccgcga
     7141 aatcgttcca gatgaaaaag aagggaaata cctcgaaccg ggttcctccg aggctcaaaa
     7201 ggtaggaatt ggcgctttgc ctgcgccgca cccactgggc gttgtcttcg aaatacggat
     7261 cgtagcggtc atagatctcg aaaaccggcg ggatgaagac cgctcctcga atttcactcc
     7321 cccgaacgtg gtcgagatag tcttccaggg attggggcgg atagaggtcc tggatgccgc
     7381 aatgggcatg ggcgtcaatg atccggaaat gcatggcttt gcctccgtct tgtattccat
     7441 ccgaacccgc acgacacggt tccaaacctg ggcctatcct gcgcctttga tcacaaaaat
     7501 tcctctttgg caaacccttt gacggcatgg acgaaaaacc gggtcatgtg gtcggccacc
     7561 gcctccatgc gtgaggactt ggattttccc gaggccggat ccaggaggtg ctcgggggcg
     7621 tgctggtaga aaagacactg gcccagaagg cccagggcgc agagacgtaa ggtctcctcg
     7681 ggcaggttcg ggggtgccaa ttgcccgagg aggaatcggg cctgttccag gaggggcagg
     7741 atagcctgtt ggaagagctc cgggaaggcg ggggagggtt ccagtagttc gcgcaggagg
     7801 atctttccgg accagggagt gttcccttcc cggagggtgc ggaagagaag ggatcgaacg
     7861 aaggcgtaga gctgttcttc aggggtcgaa ccgtcggcgg cccccccctg gggaggcggg
     7921 gttgaggact cccgggaaac gaggtttcgc acggcggcgg agtataagcc gaacttgtcc
     7981 ccgaagtggt aatggatggc tgcaatgttg actcgggcgc ggcggcagat ttcccgaacg
     8041 gtgacggccc ggtatccgtg acgggcgaag agttcccccg ccgtttccag gagtcgggcc
     8101 ggggtttggg tgcgggcctt tttttcggac ttcatcctcg cctcctggaa aatgtcggac
     8161 actagtttaa atcatgtgtt tgaacctgta aagaagggcg ggcgaagaaa actcgaaccg
     8221 gacgacggtc cgtcccataa aaaaagcgta aacaaaatcc cggagggttc cgttactacg
     8281 gatgaaaggg gaaagatatg aaactcgagt gcaaaaatcg tccttccaat ccccgacgct
     8341 tccccggcgg atctccggag gcgctttccc cgtccccaaa aagcagtcag gcacaactat
     8401 tgcaaatcga ttggtccacg gacggcccgc cccggcggtc cgtcccggag gaatacggaa
     8461 accgtgccgc cgaaccacag ggaaaccccg gggctccatc cccctgcggt ttcgaagacc
     8521 cttcacggat caaggactcc ggtcatgaaa ttccagtgca tccgatgcca tagctcctgg
     8581 ggagaaggaa tcccggaaga ggaagggtac tctcacggcc tgtgcgctcc ttgcctcaaa
     8641 gaggccctca cgccccttta ccgcaagcga cagatctccg aaggaaactt cgactgcttt
     8701 ggaaggtccc aaggctactg cgaccaatcc gcctgcaagt accgggagat ctgcctctga
     8761 gccccctccg ggagtccata agagcctcca cggatttctt ccgccaacga cgcggctcga
     8821 tctcacgcct ccgagtacgg caaaacggag gcccccgccc cctgcaagtc ccgagatctt
     8881 tcccccccga ctttcaccca aaggggttcg gccttatttt atccctgcag cggcagccga
     8941 ttctatccgc aaattcggaa tccgctccgg cggacaagga ggcatcatga tctcgttgaa
     9001 accacccccg caaaggggag attcaaacgg gtgcgatggt tgtggggttg tctcccctga
     9061 aggcggggct tcccacgatt gcgcccaaag aaataccgtg ttcacgacaa gagagcttga
     9121 aatcttcgcc cgaatccggg aattgggcca acgggcaaga aggctcaaac aagctctgga
     9181 gcatgccggg gcggcccggg gtccggaagg acggactcga gaggatcttc tcgatgaatt
     9241 ggaaggcctt cgccgggaac gaaagcgggc ggaatcggaa cgcatcgacg cccgggcgga
     9301 acgcatgcgg ctcctggggc acgaataggg tttccatccc ccgagcatat taggaatagt
     9361 ccgtgatctt cttccctttt cttgcttttt ctcctatccc ggtgtaacgt tacgccgagt
     9421 cccttccccg cgagggggct tctcaggaca gcagccgaag gggtgctctc aatttgaggg
     9481 ctgagatgat accctcgaaa cctgatccag atcatactgg cggagggatc ggctggcgca
     9541 caatgcctca gtcggccgtt tcctaaaacg gctttttttg tttctgaacg gttggtttcg
     9601 ggaaggatct tggggttgcg cctcccatcg cccacaacaa ggaaagaccc tatgcgaact
     9661 cagttggatt atgcgaaagc gggaatcgtc acggaagaaa tgaggcaggc ggcggcggtg
     9721 gagtccgtct cccccgagga gcttcgggac ctcatcgggc aggggctggc ggtccttccc
     9781 aagaatgtcg gccattcctt tcctattctg cgagccatcg ggaagaatct caagaccaag
     9841 gtcaacgcca atttgggcac gagcggagaa tgtgccagcc gggagatgga aagtgccaag
     9901 ttgaaggcgg ccctggatgc tcaaaccgac tccatcatgg acctttctac tgggggagac
     9961 ctggccggat ttcgtcactt cttcctggag aattctcccg tcatggtggg ggccgtgccc
    10021 atctattccg tcgccacgga gatggtggcc aagcatgagc ccctggacac catggacccc
    10081 gaccgccttc ttcgaagcat cgaaaaacaa tgtgccgagg gactggacta catcaccgtc
    10141 cattgcggag tcaccctggc ctcggtgaag aagctggaga ccttcccgcg catcatgccc
    10201 tgcgtcagcc gcggggggtc catccacatg cattggatga aaaaaaacgg caaagaaaac
    10261 cccctgtacg aatactatga cgacctgttg gaaatcgccc accggcatga cgtgaccctc
    10321 agcctggggg acgggtttcg tccggggacc atcctggacg ccatcgacgg ggcgcagatc
    10381 gaggagctga tggtcctggg ggaactggcc cggcgggcgt ggcaaagggg ggtccaggtc
    10441 atgatcgaag ggcccggcca tgtccctctt caggaaatcc agtcccatat tcaactgcag
    10501 aagaggttgt gccggggggc gcccttctac gtgctggggc cgctgcccac ggacatcgcc
    10561 gcgggatacg atcacatcac ctcggccatc ggcggggccc tggccggagc cgcgggggcg
    10621 gatttcctct gttatgtgac tcccgccgaa cacctgggcc tgccggtgga ggaggacgtg
    10681 tatctgggag tgatggcggc acgcatcgcc gggcacgtgg cggatatcgt caagggagtg
    10741 cccggagccg tcgaacggga tcggcgcatt tccatcgctc gccagaacct ggattgggaa
    10801 ggggtcatcc gggagtccct ggacccggaa ctggtgaagc ggcgcctgca gatcacccag
    10861 gaccgggaag cctgcagcat gtgcgggaag ctctgcgccg tgaagatcag ccgccaggtg
    10921 caaaacgtct aggcccccca ccgccgggcc ccggtccctt tcccccctca aaggggcgga
    10981 gggtctcaag acaagccctc ccgtcgggag gattccccgg gccaaaagct cccccgcggg
    11041 caccgtgacg gttcgacccc ggagggttcg ggagcaggag tcgcattcga cacgtcaggt
    11101 ttcgagcgga gggagttcca tgggcagacc gacgtataag gaactcgagc ggcggatcca
    11161 gaaactggag caactcgtta acctcaaggt ctatccccag gaggtccacc aggaaggacg
    11221 gccccggatt tactttcgca tgggagccga ctggactttc gattttttcg acaagggaat
    11281 ctccgatctc acgggctacg acctggaaga ctttctggat cgacaggttt actggttgga
    11341 cctgatccac gaggaagacc gggacccgct cctggattcc atccgggtgg ccatggaatc
    11401 cgatcggtac tgcctggccg aattccgcat ccggtgcagg acgggacaaa cccgctggat
    11461 gcgaatgtcc ggccccatct tctgcgacga ggacggcaga ctgcagcata ttcaggggat
    11521 cctatgcgac atcacggccc agaagagcat ggaagtgacc ctggattccg agcacgaagt
    11581 cttcgtctgg gtggccaaca gcctcgagga cggcatctac atcgtttccg acgattatcg
    11641 catccagttc atgaacaagg ccctggtgga gctcatggga aaccacgtag gccggatctg
    11701 ccacgaggcc ctgtttcacc gcccatcccc ctgtccccgg tccccctcgg actccctcca
    11761 ccacgaaggg tgcggcattc accagtacta catgggggag ttgggcagga ccttcgaggt
    11821 gcgcagtttt cccatcaaga cccgctacgg gacgggcaag ctgggaaagt tcaaggacat
    11881 cacccgcacc aagatgctca agcacaaggt caaacacctt gccgtccgtc accaggcaat
    11941 cctggacgcc gccaacctgg cggacatcgg catctttcga ctcgaaaccc acgagggcat
    12001 cgaggcgagg tttcgctgcg ccaacatcgc tttttgccgc atcaccgggt atgccgcaga
    12061 ggaactgctc caaatgagct ttctcgacct cattcccgag gcatttcggg aagaggtgct
    12121 ggatctctac cggcgcaggc aacggggaga gatccccaac gaaagctatg agcgcaagat
    12181 ggttcgcaag gacggatccc tcatcacggt caatgtcagt gttgcggcca ttcagaccac
    12241 cagcggacga gtcgccacca tcgggtttct ccaggacgtg accgaaaggg aaaaatatca
    12301 aaaatccctc tggctttccc agcggctctc ctccatcggc agggtggccg cggaggtcgc
    12361 ccatgagatc aacaatccct tgaccagcgt catgaccttc aacaagctgg tggaacggat
    12421 catccagcag gagccgtttc cagtccagag aacggccgaa ctgagggatt acacccgcta
    12481 cctcaatgcg gaagccagtc ggtgcgccga aattgccagg aatctcctcg aattttcccg
    12541 ccataacgaa atcgagatca aaaaagaaca ccatatccat gaaatcctcg agaagaccct
    12601 caatattctg aggcatcgag cggccctcac cgatattcgc atcctgacct cctatgccga
    12661 ggacgttcca cccatgcttt gcgacttcaa gagattgcag caggcctttg tcaacattct
    12721 ctggaacggc atcgaggcca tgcccgaggg gggggtcctg tcggtttcca ccaattacga
    12781 cgctcaggaa cgcctggtga gcgtggtcat cggcgacacg ggggtcgggg ttcccgagga
    12841 ggacctggac aacatcttcg agcccttcta tacgaccaag gccgagggga agggagtcgg
    12901 tttggggctt tccgtagcct atggaatcat tcgccaacac cagggccgga ttcatgtcca
    12961 gagcatggtg ggcaaaggga cccagttcac catcctgctc ccggcggatg ccctctcggt
    13021 ctcggaagtc gccgaatgcg gggaatgacc cgcaaagcgg ccttcccacc ggctgaagcc
    13081 tcctgccccc gggaggggaa acatcccctt tcccttccgg accgggaaaa ggcgaaccgc
    13141 ccgttcgcgg caggaccccc aagcggctgc gggaccggcg gggagacggc gaggccccgg
    13201 ggatttccgg caagcggcaa ctggggggga atcccgccgg gaagggacgg ggatcgaggc
    13261 tgtttggagg agggattctg cgaccaatac cgtggattgg aaggtaccgg aatgaaagaa
    13321 gacgctcgta ttcttgtggt ggatgatgaa cccatggtgt gcctgtccct ggtgaactgg
    13381 ctcaaggagg agaattacct cgcccaatcg gccgaagacg gtccgtccgc cattgccgcc
    13441 gtgagccagg aaccgtggga cgtggttctg ctggacctga agatgcccgg catggacggc
    13501 ctcgaggtcc ttcggcaggt caagaagatc tccccccaaa ccgtggtcat catgatgacg
    13561 gcctacgcat ccatcgccag ctcggtccag gccatgaggg aaggggccta cgactacatc
    13621 atcaaacccc tggacgtcca ggaactgacc ctcatgctca agaagatcgt ggatcaccag
    13681 aggctcgtca acgagaacat tctccttcga aaaaagctca ccgaacgata cgcctatgaa
    13741 aacatcatcg ggaagagcaa cgccatgcaa aaggtcttcg aaatgatccg cacggtggcg
    13801 gacacgagcg ccaccgtctt catcagcggg gagaccggca cgggcaagga actggtggcg
    13861 cgggccatcc acaccaacag ctcccggaaa tacaaccctt tcatcgccgt cagctgcggg
    13921 gcgcttcccg agaccctcct ggaaagcgag ctgttcggct acgagaaggg ggcctttacc
    13981 ggggccgatc gaatgaaaaa gggtcgtttt gaactggccc acggcggcac cctgttcctg
    14041 gacgagatcg gggacatcag catgcgcacc caggtgaagc tcctgcgcgt cctccaggaa
    14101 aagaagttcg aacggctggg gggaaccgaa tccatcgaag tggatgttcg attgatcacc
    14161 gccagcaacc gggaccttcc caaggccatc gaggcggggg atttccgaag cgacctgtac
    14221 taccgcctca atgtggtcaa catccacctg cctcccctgc ggcaaaggac cgaagacatc
    14281 ccgttgctca tcgagcactt catgcgcaag ttcaacctgg agttcaacaa gaagttcact
    14341 cgggtggaag acgcggccct gaacctgatg atggaatacc gctggccggg aaacgtccgg
    14401 gagctggaaa acgtgatcga acgcgccatc gtcatcgaca acggcccgga aatcaaagtg
    14461 gcccagctcc ccttcttcaa gaaggacgag gcctgcgccg aaggggggcc cctgacgttg
    14521 gaagagatgg aaaggaccca tattcaaaag atgctggatc tctgcaaggg gaatgtgtcc
    14581 aaggcggccc ggaccttgtc catcgatcgc acgaccctcc acaagaagat caagcagtac
    14641 ggcctgtcaa aacggtagcg acccggcatg atcgaccagt cgcccgccat tcacctggtg
    14701 atcctggaag ccgaggagcc ggactatctg aggcaggtgg cggagagtat ccaagaacag
    14761 ttcgacttgg acgtgaccgt cggcaaaccc ctgggattgc cgacctatgc cctggacccc
    14821 gtccgaaagc agtacaactc caacctcatc ctcaagaggc tggcggaaac gtgccctccc
    14881 cacgccgtca aggtcctggg cctgaccgcc gtggacctgt tcagccccat tttcacctac
    14941 gtattcggcg aggcgctgtt tcaggggaaa agcgccgtga tttcggcctt tcggcttcga
    15001 ggggtccccg acgccaaggc cgccctcggc tgcccgcccc tgatgaatcg catggaaaag
    15061 gaggccgtcc acgaactggg ccacaccttc ggtcttcgcc actgcctgga ccccgattgc
    15121 gtcatgaact actcctcggg ggttcagtgc gccgaccgca aattcccctt tttctgcccc
    15181 gcctgtcgag aactgctcct ctggtccctc aaaaaatcca tgcgggaata gccttcgaat
    15241 cctcccgtct cccccggcca aaccggactg tggttttcga ccacatcctg tagaaagccg
    15301 catcacccat gatttataga ataaaaacca ttccttatcc tcgattcgac cccagggagg
    15361 tgtggtcgaa tcccccaagg ctcgaagaag cccgccgagg tcccgccaag ggcacataat
    15421 gcaatactct cgtcataacg ataggttagg gtctttccgg tcttcctggc acggaggttg
    15481 ctccagggta agggcgaccg tgaagaaaaa aaacgggaaa acaaaccgaa aatcttgcag
    15541 acgatagaag gagaaggcca tgaaagaaaa gactaaagag aagagcaaag tgaccgcctt
    15601 caagacggtt ccgtccggcg aagagaaatg catctggatg gaagcagggg tcctggacta
    15661 caaactctgc aacaactttt acaactgtca tacctgtgcc ttcgacaagg ccatgaaaga
    15721 aaccgcggag agaaaccttc tggccaaggt ggagcgcatg gagcctccgg ggaagaagtc
    15781 caacatcgtg acctggcagg agagcatgag gaagcggggc gggctggaac gcaagtgccg
    15841 ccactacctc acgggccggg ctcccttccg cctttgcccc tacgacttcg agtgtcacgc
    15901 ctgcgggttt gaccagatgc tcgaggacgg gctcgaactg caacttccct accccgtgct
    15961 ccagatgccc ctgatcgacg gctactacgt ccccgaggga catttcttcc acctgggaca
    16021 cgcctgggct cgagtggagc aagggggacg ggtgaggatc ggactggacg acttcagcat
    16081 gagggtcttc ggcactccgg aaaaactgga actgcccttg acgggagagc gggtccggtt
    16141 cagcgacgtc gggctggcct tcaaacgcat gggaaaagag gcttcggtcc tggcccccct
    16201 cagcggcatc gttagcgccg tcaacgccca agcggccaag gaaccggacc tggttcgcca
    16261 tgaaccctac aacaagggtt ggctcatggt gatcgagccc acggacatga aaaagaacct
    16321 caaagagctg ctgtacggga agcagaccac ggaatggatt cgttcggaac accagagcct
    16381 ggtggaaatg gtcagtgagg tgggaatcac cttcgccgac ggcggccaga tcgaggatgt
    16441 gttcggaaac gtgcccaacc tcgcctggga gaagcttgcc cgccgcttcc tgcgcaccta
    16501 ggggcgtcgg catcgggttc gatgccgacc ttccttccgg cccccggcct tactcacccc
    16561 ggcgtcctcc gcaagaggac gccggggtgt ttcgctcctc cccgcccgcc gtcggggccg
    16621 ttgcccgcgg accggacggg caacgcgttt cgagatcttc cgaggggtcg ggccgtcagc
    16681 gaagggcccc ctcccagtct ccctctttcc aatccgccgc cgaagccagt tcgtagagga
    16741 cccgagccgc ctgctcatcc ctctgttcga aagcccgatc ccgaatataa ctgtacattt
    16801 ccagggtgca ctcgtctctg gcggtcttct tctggaaaat gcagtaggag aggtaggatt
    16861 cgaagtcgct caccgtggtc tcgttccggc aatgctggaa aaattccgca aaatccatgg
    16921 gacctcctga ttcaaaatgg aatggatgag gaatcaagaa ccttgattgg ccccactata
    16981 tcccaaagaa agcccttgcg caaagaatcc cttggccccg gggttcgagg gaggattctc
    17041 atggcagggg ggcccggaag tctcttccgc ggatttcgcg cccccacagg gcccaccggg
    17101 gggctccaag gcccgcggcc ggacggggcg gggttggccg gcccgggaag catcggactt
    17161 tttccgcccc catgcaggga atccctcctc cggatccgca ggttttcggc gaaattatgg
    17221 cgggaccctg tttagaagct tgacggagag cccctttttt gcttttatgc agggcgaatc
    17281 ttatgtgccg gtccggtgga ccccccgctc cgacctcgaa gaaacgagtc ggcccggccc
    17341 gggcagagat tcgcgggagc cattgcgaga tccggatgtc cttgatgggt tcgtgccgaa
    17401 ctgaagagag cacgcgcgcg ttgtttgcag cttgccatgg atctgaaccg gcgattcaga
    17461 gcaagaggac gatcaactgc tcgacccctc cgcttttccc tctcctgatg gattcacctc
    17521 ccctgaacgc ttcccccaca gctcgatccg gaggagcatt ccccgggaat gagaagcatt
    17581 cggcggacgc agagacgtag aaacgatttc tttgaccagc ttccatgccc cacgccgccc
    17641 ggctccccga gatcgcctgc catgattgtg caagatggac gaaaagtgca ctcccgcggg
    17701 gattgtcctt gaagagcgtt ccatggggtg atatgttcag ctgcaatgtg cattggcagg
    17761 ataccttcca tccggaactg gaggggatgg aagagcgagt cggttcctaa gtcctcgaaa
    17821 tgagccacaa tcacatggga atgggtatga tgcaaagcaa aacaggtgaa tcgcaacaaa
    17881 gacaagctcc gggttccagg aaagacccgc ccgtcggcgc cgtcatggtg gtgggcggag
    17941 gagtggccgg aatccaggcg gccctggact tggccaatac gggcttctac gtctacctcg
    18001 tggaaaaatc ccccgccatc ggcggggtca tggcccagtt ggacaagacc tttccgacca
    18061 acgactgttc catgtgcatc ctgtctccca aattggtgga ggcgggccga cacttgaaca
    18121 ttcggttgat gacccgggcc gaagtgcagg agttgaccgg ggaagtgggc cgcttcaccg
    18181 caacggtgcg cgagcgggcc cggtacatcg acatggaaaa gtgcatcgcc tgcggggtgt
    18241 gcgccgagaa atgtcccaaa aaggtggagg acgagttcaa cgaaggtttg agccaacgca
    18301 aggccgcctt tgtcaagtac ccccaggccg tccccctcaa gtactccatc gacgaggaaa
    18361 cctgcatcta ctttgccaag aagggcaaat gcaaggcctg tgaaaaattc tgtcccgccg
    18421 gggccgtgga tttcgcccag gaggacaaga ttcaccgcct ggaagtgggg gccgtcatct
    18481 tggcaccggg cttccggacg tttcggccgg aagcctacga ctacgggtac gggacctatg
    18541 ccaacgtggt gaccggcacg gagttcgagc gggtcctcag cgcatcgggt ccctttgaag
    18601 gccgcctggt gcgtccgtcc gacggcaagg cccccaaaaa gatcgcctgg ctccaatgcg
    18661 tcgggtcacg ggacacccag aggggagccc acggatattg ttccggcgtc tgttgcatgt
    18721 atgccatcaa agaagccgtc atcgcccggg agcacgccca cggagatctg gacaccgcca
    18781 tcttcttcat ggacatgcgc acctacggca aagatttcga gcgctattac gaccgggccc
    18841 gggaggagag cggagtccgc ttcatccgat ctcgagtgca cagcatcgag cccgtcgctc
    18901 ccggctcgga cgatttggcg atcagttacg tgctggatga cggggtcccc cgcaccgaag
    18961 tgttcgacat ggtggtgctc tcggtgggca tcgagacccc ccgggacgtt caggacctgg
    19021 ccgcccggct gggggtggag atgaacgtcg acgggtttgc cgaaacggca agcttcgctc
    19081 ccgtcgccac atcccgaccg ggagtgtttg cctgcggggt ggccacgggt cccaaggaca
    19141 ttccctattc ggtcatggaa gcctcggcgg cctcggcggc ctcggccgca gccctttcct
    19201 cctcccggca tactctcacc aggaagaaga cctatcccga ggagcttcct gtggccgaag
    19261 gtgagccgcg catcggggtc ttcgtgtgcc actgcggcat caacatcgcc agcgtcgtgg
    19321 acgttcccgc cgttcgcgaa tacgcgggga cccttcccaa tgtggtgtac gtggccaaca
    19381 atctcttcac ctgcagccag gacacccaaa agctcatccg ggaagccatc gaggaacatc
    19441 gcctcaaccg ggtggtggtg gccgcgtgca ccccgcgcac ccatgagccc ctattccagg
    19501 aaaccattcg cgaagcggga ctcaatcgct acttattcga gctggccaac attcgggacc
    19561 aagattcctg ggtgcaccag gcggagcccg agaaagccac ggagaaggcc aaggacttgg
    19621 tgcgcatggc ggtggccaag gtggcgctcc tcgagcccat cgaacgtatc gaggtggatc
    19681 tcacccacag cgccctggtc gtcgggggag gtgtggccgg aatgacctcg gccctcaacc
    19741 tggccgacca gggattcggg gtcgtcctgg tggaaaccaa ggaatttctg gggggacatg
    19801 ccctcaaggt tccccgtacc tggaaagggg aggacgttgc ctcccatgtc gaggacctcg
    19861 tccggagagt cacgaatcac gaccggattg aggtgatcac cggggcaaag gtgaccaagg
    19921 ccgggggctt tgtggggaac tttttctcca cggtggagac cccccagggg gagaaagaaa
    19981 tccggcacgg ggtggtgatc ctggccacgg gagcccattc cctcaaaccc gacgagtacc
    20041 tgtacggcca aagcgaccgg gtcacccggt ggcacgagct ggacaagcta tttcgggaag
    20101 agccccgaag gctcgaggag gccgaggccg tcgccttcat tcagtgcgtg ggttcccggg
    20161 aacccgagcg gccttattgc tcgaagatct gctgcaccgc ttccgtccag caggccatcg
    20221 agctgaaaac ccgcaaaccg gacctggatg tcttcatcct ttaccgggat cttcggacct
    20281 acggaccgag ggaagagctc taccgaaggg cacgggaact gggagttctc tttctgcgct
    20341 attccttgga cgccaagccg ttggtggagc ccgttaccgt gaacggacgg gagaagatcc
    20401 gcatcaccgt gcaggaccat gtgctccagc gccccgtcca cctggaggcg gattacctga
    20461 acctggccac ggccatcatc cccaaagact tggaaagcct ggcccagttc tacaaggtcc
    20521 ccctcaacca ggacggtttc ttcctcgagg cccacatgaa gcttcgtccc gtggatttcg
    20581 ccaccgacgg ggtgtttgtg gcggggcttg cccactatcc caagcccatg gaggaatccg
    20641 tggcccaggc ccaggcggcc gcatcccgtg cggccacggt cctgacccag gccaaggtgg
    20701 aagtggaacc catcgtatcc tgggtcgatc aggaggtctg catcgggtgc ggactttgcg
    20761 aggtttcctg cccctttagc gccatccggc tgaccaaggt ccacggaaag ggatatcgag
    20821 ccgagaacat ctcggcctcc tgcaaaggct gcggcatatg cgccgcctca tgtcctcaga
    20881 aggccatcga catgaagcac ttccgcgatc gccagatcat tgcggccatc caggcgggcg
    20941 gacaaggata ggcaaaaacc gcacacaggg gagcttcctg cgggagccct ccaaatgagg
    21001 gacggcaccg acagcggggg gcttcctcgt ctttggaagg gagagaaaca catgagcaag
    21061 gtttttgaac ccaagatcct cgcgttcctg tgcaactggt gctcctacgc gggggcggat
    21121 ctcgccgggg tctcgcggtt tcaatatccc tcgagcatcc gggtaattcg gaccatgtgc
    21181 tcgggccgcg tggacccgct ctacatcacc gagggattga aaagcggctt cgaccaggtc
    21241 tccgtcttcg gttgtcacct gggagactgc cattacctgg atggtaatgt cttcgcctcc
    21301 aagcgcatgc gactcgtgga acacctcctg gacctgtccg ggatcggccg aacgcgaatg
    21361 gcccttcgct gggtgagcgc cgccgaggga cagatttttg ccgacaccgt aaaggaactg
    21421 acggaaacca cccggaatct ggggcccttc gatcgggttg ccatgggcct ccgactggcg
    21481 gccgtggaga ccgtccttcg gggtcccagg atgcgatggc tgacggggat ggaccgccag
    21541 atcaccgaac gagggaacgt gtaccacgac aaaatggacg aatccctgtt tcaggaaatg
    21601 ttgaagctgg cggcggaaga ggaataccag aaggctctcc tcctggaatc cctcaaagag
    21661 ggtccccttt ccgtccgaga aatggctgca aaaacgggcc tgcccgtata caccgtgtcc
    21721 ttgcgattga acgacctcga gaggcggggg caggccgagt tgaaggaata cgaagggtcc
    21781 accccgaaat tcgtagggtt ggcggcatag tctgggaggt ggtatcgggc ggacggaggg
    21841 gggagccggt gagaagctcc ggaacctcga tccgggttgg aggcccgagg cgcgaaaggg
    21901 aatgcggaat atggcaaatg aacagaatgc gaagaagata ggcgccgtga tggtcgttgg
    21961 tgccggggtg gccggaatgc aggcgtccct ggacctcgcc gaatccggct actatgtgca
    22021 cctggtggac aagtcgccca ccatcggcgg catcatggcc cagttggaca agaccttccc
    22081 caccaacgac tgcgccatgt gcatcatttc ccccaaactg gtggaagtgg ggcggcactt
    22141 gaacatcaac atcctgccca ataccgaagt ggagtccgtc gagggggagg ccgggaattt
    22201 caaggtgacc ctccagtgtc agccccggta catcgacctc gaagcctgta cggcatgcgg
    22261 ccagtgccgt ttggtgtgcc ccgcaacggc ggtgaacgag ttcaaccaaa agctggacgt
    22321 gagggaagcc acctacatgc ggtatcccca ggcgattccc cggggcttca tgatcgatcg
    22381 ggccgtgtgc atcgggtgcg ggctgtgtga aaaggtatgc ctggccgaag ccgtccatta
    22441 cgacgacacc gtgcgaaaaa cggaaatcga ggtgggcgcc gtggtgttgg ccccgggaaa
    22501 cgaggtgttc gacccttcgc agttcgacac ctacaactac ctgggatttc ccaacgtggt
    22561 taccagcctc gaattcgaaa gaatcctgag cgcttcggga ccctaccggg ggcacctcat
    22621 gcggccctac gaccgggaag agcccgccaa gatcgcgtgg ctccaatgcg tgggttcccg
    22681 aaacatcaat gcctgtgaca attcctactg ttcggccgtc tgctgcatgt acgccatcaa
    22741 ggaagcggtc atcgccaagg agcatgcgca cggagacctg gataccgcca ttttctacat
    22801 ggacatgcgg acctacggca aggaattcga acagtattac aaccgcgccc agcagcacgg
    22861 ggtccgcttc atccgctccc gggtccattc ggtggaaccc gagggagagt gtgacctgag
    22921 gatcacctac gtggatgaag ccggggaaca gaagagcgag gtcttcgaca tggtggtgct
    22981 gtcggtgggc ttcgaggtgg ggctgg
//
LOCUS       DAOOVC010000054        22581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1564_length_22581_cov_6.093536, whole genome shotgun sequence.
ACCESSION   DAOOVC010000054 DAOOVC010000000
VERSION     DAOOVC010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 22581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22581
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1564_length_22581_cov_6.093536"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLM79_08860"
     CDS             complement(<1..202)
                     /locus_tag="PLM79_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86459.1"
                     /translation="MTKVLISEMEPNQDIATCLVVTEKQLRTARNGSLFLTLKLLDKT
                     GEITGRVWENAEEVCNAVPVKTA"
     gene            671..2287
                     /locus_tag="PLM79_08865"
     CDS             671..2287
                     /locus_tag="PLM79_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOV86460.1"
                     /translation="MLESIPQKIRALVYGEASRSAWIAVVGGTGTGKSTVFNALCDHR
                     LSATGFDRPKTRGAIAYVHKDNPVEQGFPFARIRRERIQAESPDNLPPAGSPGELLAV
                     EHDRAEYSHLVLVDTPDLDSHELKNRRIVDELALVADVFVFVSSQEKYADQTPFEFLH
                     KIRETGKPVFVLLNKAESVFGVEDARSVLGKEASAVSRDRLWLLPYLPSETGRFLSQS
                     PGFREFARALFRETDTRRTDSLLSRSRSLRRADLRKDLGRLLGLLEEEKRAAEGWKTA
                     LEAFFRSECEALLRQEEKRFTERSREVLQREIRALYDKYDLLGKPRRFVARVLGAPFR
                     YFRTPGEEVPKSRAEHLARIRERFDLEPVLSSIEHFNRCVLENLSPPDPTAPIHGALR
                     RETLPLTRAEIEAKARTDQERLLAWLEETFQQMSRGLPKSKEWGIYSASILWGALILA
                     LETALGGGIGLMQAVLDSVLAPFVTKGTVELFAYHEVENIARELNQRYRQALFASLVE
                     QKNRYRSVLDGLTTSEKTRAELKSLLSSLD"
     gene            2312..4090
                     /locus_tag="PLM79_08870"
     CDS             2312..4090
                     /locus_tag="PLM79_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOV86461.1"
                     /translation="MSAETEERGDCRDGGTGDRVPDLRVRLRAVQAYLGRGTALSLGT
                     EERERLLRECGRLLDELDRCDESILTVGLLGGTGVGKSTLMNALAGEEISSVSHRRPH
                     TDQVILYHHRSVPVPPIEGKEEIRFRAVPHEAGSMEQVLLCDLPDFDSLVGDHRKSVL
                     RFLEHLDVLIWVVSPEKYADAKFYDLLAEVPKARQNFYFVLNKIDLFFEESPAPGREA
                     GYEAMNRVAAGFLEKLRQVGFDDPMLAVVSAREPREGRVPLSANQLSSLRRQIYRERD
                     RKEIQVIKASNVAEGVRRLSSPLDREALSLKSCLRVLEEIAGFLDQERSAWRELGERL
                     FSRWLNEDLRSWMEVTFTRPDALVGAGRGWCGIVLEWKRAVGRLGGTAASIPELEDPE
                     PLHLLEQRWNHLDDHIQHRLLRAGISPQWLADTATDVVNPARWADIRLRLQEHLRNRT
                     EGPRDPGLRMFKILQYAGYLALFLAFLFSLAAEVLQRQPGTPFQPPSWTDLLWALLLQ
                     LFGPGGLCALLSFLLLQGALGWWFYMRYKKYLRRRVQKFIESLSRELAGIWVDELDAR
                     RDLLKECSRRVEERLAEITFTVQENP"
     gene            4091..4312
                     /locus_tag="PLM79_08875"
     CDS             4091..4312
                     /locus_tag="PLM79_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HOV86462.1"
                     /translation="MDMPLKLVLLIAIFLLPLAPTFWAIVDIPKRRFATSKKKLLWFV
                     TVSTLPFFGAVLYLLIARRHTTPMEISVG"
     gene            4331..6226
                     /locus_tag="PLM79_08880"
     CDS             4331..6226
                     /locus_tag="PLM79_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOV86463.1"
                     /translation="MLDFVRKHATSWLVKVALFLIIIVFIFWGGYTYQSLKENQIAQV
                     GDQFISIKDYNDTYNRLVENYRRQLGPAFNEEMMKRFNLKQQALDQMINRILILRASR
                     EMGIAVTEREIQQKVLEYSVFQADGKFDQKRYVGILRQNRLTPEMFEQQLADEIVWQK
                     VEAFIKRRALVTEEEVLADFRFNQTRVQLAYVIIDPRDYESRVTVDDASLQAFYQEHR
                     EEYKDPEKRQVSYAVLPLDDFLSAVKVSEDEMRQYYEDRPDSFRYEEEVRARHILIEV
                     KPDASEEDMAKARAEAEKVLAQARKGVDFAELAKKHSKDTNSAKKGGDLGFFTRQKMV
                     REFADAAFALKPGEIGDVVRTEHGFHVIKVEEVRPERVESFEEAWGRIEESLKKEKAR
                     DLAFRKARDLSDLAYAQRDIKKAAQSMDLAGKIREESLGAKDALPGLEPPSPEIVRKI
                     FAVPDKGTTEAMEFVRGFVVAQVLSVTPPEVLPLEKVKDRVEKELRARKARQAAEKDA
                     SEMLEEARKLHSLEQAAKTRKLELLRTESFSRSNPDKKLKLPPESTNAVFQLDSDRPF
                     PEAPLDLRKRVFVCQLLEKTVPSEIPENERLSVAKRIQMQKQAMVWQAWMEEQRGRAK
                     IEQFREL"
     gene            complement(6221..6877)
                     /gene="coaE"
                     /locus_tag="PLM79_08885"
     CDS             complement(6221..6877)
                     /gene="coaE"
                     /locus_tag="PLM79_08885"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOV86464.1"
                     /translation="MSEKRKVALTGGIATGKSTVAAMFARRGALLLDADQAAREVVSP
                     GTPCWSRLRSLLDPVYFGARGNLERRALRERIIRDPVCRQEVSSILHPAIMEWMETRW
                     AEALESRPGRPVVFDIPLLFEADLHSRFDVIILVYVPAAVQVERLMARDGVSLEEARE
                     TLAMQLPIESKRARAHFLIDNSRDPEHTRHQADTVFEAVFPDFSRQGPPPSSPSPASL
                     "
     gene            complement(6933..7265)
                     /locus_tag="PLM79_08890"
     CDS             complement(6933..7265)
                     /locus_tag="PLM79_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86465.1"
                     /translation="MKRGICWVVMVSLFAWFFVLPVRADEPQRSSSTGAQIDERVKWD
                     PPGELILADVLILRPLGIAALGIGLAGSLVTMPFAATSNSGDRVGKELIQKPFEYTFT
                     RPVGDVVY"
     gene            complement(7319..8545)
                     /locus_tag="PLM79_08895"
     CDS             complement(7319..8545)
                     /locus_tag="PLM79_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOV86466.1"
                     /translation="MHYRDELKVKAERALHKKAAIGPDVDLEQFDKTSDPHAYLTDDK
                     LRELPEGDRQRLLMSGVDVSEQDRAGTYFQKDTTVVHCKSKQEGIEVIPIRKALEELD
                     WIWDYYWKLASVDTDKFTAEAELNLHNGYVIRALPGAKSIYPIQACLYIDKEGLQQNV
                     HNIIIAEEDSELHIITGCSTSPHLTKGIHVGISEFFVKKNAKLSFTMIHNWAEDMAVR
                     PRSVGQVEEGGLFLNNYICMKPVKTLQMYPTTHLVGENAVARFYSVIVGTPGSEYDVG
                     GRIFLKKRGTRAEIVARTISNGGRIISRGHLIGEVPEIKAHLECRGLMLNGGLTYAVP
                     ELEGRVDGVEMSHEAAVGKIAQEEIHYLMSRGLSEEEAVATIVRGFLSVDIPGLPPQL
                     KAEIDKAIELSEKDVM"
     gene            complement(8524..9291)
                     /locus_tag="PLM79_08900"
     CDS             complement(8524..9291)
                     /locus_tag="PLM79_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86467.1"
                     /translation="MLVVEDLQVELAGKVILRHIDLEIKPGETHVLFGPNGSGKTSLL
                     MTLMGYPQYKVIGGKIVFKGVDITHSPIDERARLGIGMSYQRPPTIHGLKTEQMVRIC
                     AGNTALDVNRLAESVNFQEFLQRDVNLGFSGGEIKRSELLQLLAQNPDLMLFDEPESG
                     VDLENISLVGHTIARLLQKECGAIESKSRLQVKKERTKMGLIITHTGHILDYVDVDKG
                     QVLYNGVLSCSSNPREILRCISEIGYEECVRCTIETN"
     gene            9593..11278
                     /locus_tag="PLM79_08905"
     CDS             9593..11278
                     /locus_tag="PLM79_08905"
                     /inference="COORDINATES: protein motif:HMM:NF039677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HOV86468.1"
                     /translation="MRVDSLRPNPTNDRSLDLADFCRRLMTLPVKARLDAILAREDAE
                     KVFAGLPVQDFYFSVVEIGPEDSLPFLALAGVDQLNYLFDLQWWKKDRVDPAAAIEWI
                     GRLARAGEEKLLAWLYQVDFELLVTLFKRWITVVPAPEDEDPLEVRDRLPLRTLDDQY
                     FWECRYPQYEDLVQGVLSLLFEVHRGFYQELLNAVYWASSVEMEEEAYRFHRGRLEDR
                     GIPDYYDALEVYRSIQPEQFAADKVVPSFPPEADSPSPSFALAMLSPKDLPAQSLAIL
                     EDGLLLDTLQMELASLATRVLVADRLPTDSFDSFRFSSGKVGAYVNLGLQLLGARDPR
                     QALGLLTKHPLEHLFRLANDRISRPARAMRKILRSGRPCGIHCLDSPWLETAELLCEE
                     TPKVMRKDPKNPGAFKEDFFRFPRDLKSARHAVEVIDAVGPLLEAPGMAQPELWAKLW
                     GRAQVSDPRDITPAVMVCTAAGNEIRRGVWSPRPFPVHEWAGIFPLLEPGRIREAISR
                     WIREVLKDPRQWERAEAYFAPVLENYALEMESFGPGNAPDPEMVRFFLFTEEE"
     gene            complement(11332..12660)
                     /gene="mtaB"
                     /locus_tag="PLM79_08910"
     CDS             complement(11332..12660)
                     /gene="mtaB"
                     /locus_tag="PLM79_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOV86469.1"
                     /translation="MSLRVAVQTLGCKVNQYESSYFLEVLTNQGYREVPFSTPAEVYL
                     VHGCGVTSKASHEACRLLRRAARTNPSALVVAAGCNAVLEGRRIAREGLATHILGNDD
                     KYDLLRRLDEPASRDHPVVSVGDPRSIGSFRAVPVRCPPGGRARALLKAQDGCDAFCS
                     YCIVPYTRGKSRSLPLEDVFAQAAVFVEEGFREVVLTGVHLGQWGRDLSPPRRFSELL
                     RRMDETVRPKRVRLSSLEVLEIRGEVLGEIRARSWICPHFHVPLQSGHPEILRRMNRK
                     YTPREYGEILRELRSLFPRASLGADVMVGFPGETPRAFEETYELVRKLPLTYLHVFPY
                     SPRPGTPASKLPGRVVGGELRDRARALRELGAHKKLAFMEEALGTWVEVMAETEIASG
                     WYGGTSENYLHVNFPSSAPIPPGTLVRVKLTNLTPRGLVGRADSGKGPAP"
     gene            complement(12683..13771)
                     /gene="mnmA"
                     /locus_tag="PLM79_08915"
     CDS             complement(12683..13771)
                     /gene="mnmA"
                     /locus_tag="PLM79_08915"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOV86470.1"
                     /translation="MKRARVAVAMSGGVDSLRAAILLKQQGHELIGLHMRLVSGPTGK
                     GLGTSPRSSPEEDLYRLAGAFGIPLRIVDLVDDFEARVIASFLSAYLDGMTPNPCVVC
                     NPQIKFGVLLKKARQLGAEFLATGHYARIATPGERNGRWGLRKARDPGKDQSYFLHGL
                     TQEQLAAALFPLGDVLKSEVFRWAEEEGIRPEIPPESQEICFIQTGSYLDFVRKRVDL
                     SRYGDRGPIVDLDGRVLGEHKGIFAYTVGQRRGLGIASSAPYYVVDLDPASNTVTVGR
                     AQDLYRQELRADRVNWVSRDRPSHPISCQVRIRNQHRPAAALLTPLENARVAVRFREP
                     QRAVTPGQAAVFYDGDRLLGGGTICRTP"
     gene            complement(13839..14192)
                     /gene="nifU"
                     /locus_tag="PLM79_08920"
                     /pseudo
     CDS             complement(13839..14192)
                     /gene="nifU"
                     /locus_tag="PLM79_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
     gene            complement(14266..14691)
                     /locus_tag="PLM79_08925"
     CDS             complement(14266..14691)
                     /locus_tag="PLM79_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HOV86471.1"
                     /translation="MYSRMLVLRFSKEIVDKPIITNLVRNYNLTFNILKAQIYPRKEG
                     IVVLELQGLRSDYEKGIQYLKDVGVQIEPVAQGIRRDDDRCYQCGACTAVCPTGALHI
                     LRPEMEVLFESERCSACELCVKVCPSRAMIVTFDKVLVS"
     gene            14953..16068
                     /locus_tag="PLM79_08930"
     CDS             14953..16068
                     /locus_tag="PLM79_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOV86472.1"
                     /translation="MHEEPFQSRRDRLRTLMRQQDLDAFLVAVPENRYYLSGYGEEDL
                     QLTESSGYLLFASQGQYLLTDFRYEEAAKAQAPGFDLVIYEEGLGKSLPELFSDLEVD
                     SLGMEGHHLTYQRYLEIREALETARPKARIVCPDGLVESLRIIKDPSEIEKIRASLRV
                     TEEVLKAVWERIRPGVREVDLAWEIESSIRMRGAQAVSFPPIVAGGPNAALPHAVPTL
                     RPIAPGETVVLDLGSKLDHYCSDMTRTCFPGNPGPGVRQIYRVVREAQLAAQSAIRAG
                     VDSVEVDAVARKIIGDAGYGDFFGHGLGHGVGLAVHERPGLRKRNPTVLEENMVVTVE
                     PGIYLPGYGGVRLENMVRVTSEGCEVLNDADLFYEFA"
     gene            16274..17494
                     /locus_tag="PLM79_08935"
     CDS             16274..17494
                     /locus_tag="PLM79_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HOV86473.1"
                     /translation="MQFQKVAFKNAEGRMLSGRLDLPVDEMPDAYAIFAHCFTCTKNL
                     NAVVNINRALAREGFGVLRFDFTGLGESEGEFAETGFSNNVEDLVAAARFLASDFQAP
                     QLLIGHSLGGAAVLQAASRIPSVKAVATIGAPFDLTDLSGHLGTAEETIRERGEAEVH
                     LGGRSFTIKQHFFDDLRRAKIPQVLENLERPLLVLHSEADNIVGIDHAVRLFQTARFP
                     KSFVSLDRADHLLSQKADSLYVGSVLASWARRYMDPPRKESRSRDLKDNRIVARIGRR
                     GYQTEILANGHSLVADEPIAVGGADTGPSPYDFLVAALGACTAMTLRMYADRKDWPLG
                     SAVVRLKHRKIHAKDCKECESKDGMMDQIEREIELEGPLDENQRSRLLEIADRCPVHR
                     TLHSPIAVKTRLKD"
     gene            17532..18872
                     /locus_tag="PLM79_08940"
     CDS             17532..18872
                     /locus_tag="PLM79_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOV86474.1"
                     /translation="MEIMRSIAGTLEILVILVLITVNGVFSMSEFAVISARKSRLQQL
                     AEEGSSSAAAALELAGSPNRFLSTVQIGITLIGVFTGVFSGATVAAGTARWLSQFEPL
                     RPFSGTIAFVLVVFLVTYLSLVFGELVPKRLALYHPERIASVMAPFMRFLSMIANPII
                     QLLSFSTESVLKLAGVRPSQEPPVTQEEIKVLIEQGTVAGVFEEAEKAMVQRVFRLGD
                     RRAGALMTPRKRVQWLDVNESPDKIRRRISKSSYSRFPVIQSKAGQVLGVVHVRDLLM
                     RSLQGQPFDLRACIKPPLYVPEDMQLLKVLERFKESGMEMGLVVDEYGTVEGLITLND
                     LLEAIVGEFSSRDTPEEPRVVQRPDGSWLVDGMLPIDEFKELVQIKKELLGEREGYYQ
                     TLGGFVMTYLGRIPRAGDRFECCGVSIEIVDMDGHRVDKLLITPGGTLEETREE"
     gene            18903..20207
                     /locus_tag="PLM79_08945"
     CDS             18903..20207
                     /locus_tag="PLM79_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOV86475.1"
                     /translation="METKPRRKVVPTMLKLYNTLSRKKETFRPMEDRRVRMFTCGPSI
                     YRRPHLGNYRSFLWEDVLQRYLEFKGSRVDRVLNFTDIEDKAISEASREGLSVRELTE
                     GIAKRFVSEARMLRIKLPPEIPRSSTSIDHAVRLIRVLLEKGYAYRHGGDIFFDPLRC
                     ESFGRLFGVDMSRWPREKRRFRKDTYPGRRWNLGDFILWHGSKAGDQPSWDTEIGPGR
                     PSWNIQDPAIITQHLGFQVDLCCGGVDNLYRHHDYTLAVMEAASGQEFARCWVHGEHL
                     LVNGKKMSKSVGNILYPEDLLENGGTPDHIRFYLLYGHHRKRINLTRERFRERCRLLD
                     DVRNRIGELMARVPLRGTPGGKPGKLGAGLLHGFQKAMDDDLNVLEATRSVIRGLGEL
                     LRIRDHGDLDTEQFLDIVAALHRIDSVLQFLLPPEHHEDEGP"
     gene            20204..20950
                     /locus_tag="PLM79_08950"
     CDS             20204..20950
                     /locus_tag="PLM79_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOV86476.1"
                     /translation="MRAPKGIVQVAGVLDAQEARMLLDCGADLLGFPLKTPGNDEITE
                     DRAREIIRSLNLENRSVLITYLNRAESLADLCGRLGVKMLQVHSEISPEEARRLRKTA
                     PDLFIIKSLIVGTDNVETLEAAVRDFSDWVDAFITDTLDPQTGARGATGNVHNWAVSR
                     RIVERSPRPVILAGGLCPENVARAVAVVRPWGVDAHTRLEGPRGRKDPLRVQSFVRRA
                     REALSRAMDREGETETVPGPIPETMESGTV"
     gene            complement(21017..22366)
                     /locus_tag="PLM79_08955"
     CDS             complement(21017..22366)
                     /locus_tag="PLM79_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HOV86477.1"
                     /translation="MKTSTGSVRSPWVLAAFLVLLAGCRYHPDSLADIRRLPQKPSHY
                     LSARTADRPLVTRSRQEVLMEEYDRAFFLPWDRGGPQVTRQQVTAQVGKYTEDPGYGE
                     NYRRHTHQWAAVIRANADLDGYPNTCRRGITVDRSHLRMLPTLRPLFADPNQPGEGYP
                     FDILQNSSIPANTPVFISHTSKDGAWCYVDSHYSSGWMDSRDVAAVDSEFVREWRGRK
                     RMALVRDRVPVHHPQGGFLFQADIGSLFPAQDGGADGTRIWVAVADSRRNAVPVSTTL
                     PQDAAVLQPLELTARNIARLADELAGEPYGWGGLYFNRDCSATLKDLFTPFGLWLPRN
                     STHQASRGGLYEDVSGLSPKEKETVVKQRAVPFFTLLWARGHIMLYLGSYEGEILVFH
                     TFWGIRTRDFRGREGRTVVGHTAVTTLYPGKELPDADPDYAPILPRIEAMTFLGQGG"
BASE COUNT         4364 a         6776 c         7017 g         4424 t
ORIGIN      
        1 ccgcagtctt caccgggacc gcattgcaga cctcttcggc gttctcccac acccgcccgg
       61 tgatctcccc ggtcttgtcc agcagcttga gggtcaggaa gaggctcccg tttcgggccg
      121 tgcggagctg cttttccgtg acgaccaggc aggtcgcaat gtcttgattc ggttccattt
      181 ccgaaattag gactttggtc acttttccgc ctgcgctttg gaatcgggtt cgttcttgtg
      241 tgctcgagaa ggagcgagtc ggcccttggc gatcccaacg gcgttttctg tatagtatag
      301 cagtcgggaa cgtgtcaaat gtccctttgg gccgcccgcc gggttgtcaa accccgggga
      361 agcctctaga atcaaatgcg cgccgcggca tcccgaaatc ccggatcgag ggacgaacct
      421 ccctgcaggc tcgtgacggg agacggcgag aaggcggggg atttccggcc gaagaagacg
      481 gccatgaccg gtggagagca ccgggtgtgc tctcgtgggg tgcggcagtc agccccgggg
      541 gctcagaatc gaggaaaatg tgcccgtttc ggattctcca aggcccggcc cggccaaaat
      601 tcccgatttc tccttgtgga accgggtcct gtacccgggt ttcacctcgg gcatccggga
      661 aatccccggg atgttggaaa gcatccctca aaaaatccgg gctctggtct acggagaggc
      721 ctccagatcc gcctggatcg ccgtggtggg cggcacgggc accggcaagt ccaccgtctt
      781 caacgccctg tgcgaccatc ggttgagcgc gacgggattc gatcggccca agacccgggg
      841 ggccattgcc tatgtccaca aggacaaccc cgtggaacag ggttttccgt tcgcccggat
      901 ccgtcgtgaa aggattcaag ccgaatcccc ggacaacctt cccccggccg gttctcccgg
      961 ggagcttctg gccgtggaac atgatcgggc cgaatactcc cacctggtcc tggtggacac
     1021 tccggacctg gacagccatg agctcaaaaa tcgacggatc gtcgacgaac tggccctggt
     1081 cgccgatgtg ttcgtttttg tgtccagcca ggagaaatac gcggatcaga ctccgttcga
     1141 attccttcac aagatccggg aaacgggaaa accggttttt gttttgctca acaaggcgga
     1201 gtccgttttt ggggtggaag atgcccgatc cgtcctggga aaggaagcgt ccgccgtctc
     1261 gagggaccgg ctgtggctgc ttccttatct accgtccgaa acgggtcgtt tcctgtctca
     1321 atccccgggc tttcgggagt ttgctcgggc cctgtttcgg gagaccgata cccggagaac
     1381 ggattccctt ctcagccggt cgcgaagcct gcgacgggcg gatctccgga aagatctggg
     1441 caggttgctg ggcctgctcg aggaggagaa acgggctgcg gaaggctgga aaaccgccct
     1501 cgaggccttt ttccgttccg agtgcgaggc ccttttgagg caggaggaaa aacgattcac
     1561 cgaacgttcc cgagaggtcc ttcaacggga gatccgcgcc ctttacgaca agtacgacct
     1621 gctgggaaag ccccgtcgat tcgtggcccg ggttttgggg gcgcccttcc gctatttccg
     1681 cacccccggg gaagaggtcc ccaagtcccg cgcggagcat cttgccagga tccgggagag
     1741 gtttgacctc gagcctgtcc tttcctccat cgagcatttc aatcgctgcg tgctcgaaaa
     1801 cctttcaccg ccggacccaa ccgctcccat ccacggggcc ctgcgccggg agaccttgcc
     1861 tctgacccgc gcggaaatcg aggccaaggc ccggacggat caagagagac tcctggcgtg
     1921 gctcgaggaa acctttcaac agatgtcccg gggccttccc aagtccaagg agtgggggat
     1981 ctacagcgcc tcgatcctgt ggggagcctt gatcctggcc ctcgagaccg ccttgggagg
     2041 agggatcggc ctcatgcagg ccgtcctgga ttccgtgctg gccccctttg tcaccaaggg
     2101 aacggtggaa ctcttcgcct accatgaagt ggaaaacatt gcccgtgagc tgaatcagcg
     2161 ataccggcag gcgctttttg cctcgttggt ggaacagaag aaccggtatc gatccgtcct
     2221 ggacggcctg acgacgtcgg aaaagacccg ggccgagttg aaaagccttt tgagctccct
     2281 ggactgaaga acgggggtgg ggggtcgaag cgtgtcggcg gaaacggagg aaaggggaga
     2341 ttgccgcgac ggcgggacgg gggaccgcgt tccggacctc cgggtaagac tccgtgccgt
     2401 tcaggcttat cttggccgag gaaccgctct cagcctgggg accgaagaaa gagagcggct
     2461 gttgcgggag tgcgggagac tcctcgatga gctggaccgc tgcgacgaaa gcattctcac
     2521 cgtgggtctg ctggggggca ccggggtggg caaatccacc ctcatgaacg ccctggcggg
     2581 cgaggagatc tcgtccgtaa gccaccggcg ccctcatacg gaccaggtca tcctctatca
     2641 ccaccggtcc gtacccgttc ccccgatcga gggaaaagag gaaatccggt tccgggccgt
     2701 cccccacgag gcgggttcca tggaacaggt cctgctgtgc gacctgccgg atttcgacag
     2761 tctcgtggga gatcaccgaa agtccgtcct gcgctttctc gaacacctcg atgtgctgat
     2821 ctgggtggtt tctccggaaa aatatgcgga cgccaagttc tacgatctcc tggcggaggt
     2881 ccccaaggcc cggcagaatt tctattttgt gctgaacaag atcgacctgt tttttgagga
     2941 aagcccggcc ccggggcggg aagcgggcta tgaagccatg aaccgagtgg cggcgggatt
     3001 tctggaaaag ctcaggcagg tgggttttga tgatccgatg ttggccgtgg tttcggctcg
     3061 agagccccgg gaaggccggg tccccttgtc cgccaaccag ctttcgagcc tgcgtcggca
     3121 gatctaccgg gagcgggatc gcaaggaaat tcaggtcatc aaggcatcca acgtggccga
     3181 gggagtccgc aggctgtcga gccccctcga ccgggaagcc ttgagcttga aatcctgcct
     3241 aagggtcctg gaagagattg cgggtttcct cgatcaggaa cgatccgcat ggagggaact
     3301 gggtgagcgc ctgttttcac gctggttgaa cgaagatctt cgaagctgga tggaagtgac
     3361 ctttacccga ccggatgccc tggtgggcgc cggccgtggg tggtgcggca tcgtcctcga
     3421 gtggaagcgg gccgtggggc gcctgggggg cacggcggcc tcgattccgg aactcgagga
     3481 ccccgagcct ctccatctcc tggaacagcg gtggaatcac ctggatgacc atattcagca
     3541 tcggttgctc cgggccggga tctcccccca atggctggcg gacaccgcaa cggacgtcgt
     3601 gaatcccgca cgctgggccg atatccggct ccgtctgcag gaacacctgc ggaaccggac
     3661 cgagggcccg cgggaccctg gcctgagaat gttcaaaatc cttcaatatg ccgggtacct
     3721 ggccctcttt ctggccttcc ttttctccct ggccgcggaa gtcttgcagc gacagccggg
     3781 gacgccgttc cagccgcctt cgtggacgga tctcttgtgg gcgctcctgc ttcagctgtt
     3841 cggccccggg ggcctctgcg ccttactcag cttccttctc ctgcagggtg ccctgggatg
     3901 gtggttttac atgcgctata agaagtactt gcgccggagg gtgcaaaagt ttatagagtc
     3961 actcagccgc gaactggcag ggatctgggt cgacgagctg gatgcgcgaa gggacctgct
     4021 caaagagtgc tcccgaagag tcgaagaaag actcgctgag attacgttca ctgtgcagga
     4081 gaatccgtag atggacatgc cgctgaaact ggtgctcctc atcgccatct ttctgctgcc
     4141 tcttgcgccg acgttctggg ccattgtgga tattcccaag cgtcggtttg ccacctccaa
     4201 gaagaagctc ctctggttcg tgacggtatc cacgctgccg tttttcggcg ccgtcttgta
     4261 cctgctgatc gctcgacgtc atacaacccc gatggaaatc tccgtggggt gaaggagggt
     4321 tatcggagcc atgctcgatt ttgtccgcaa acatgcgacc tcatggctgg tcaaagtcgc
     4381 tctcttcctg atcatcatcg tgttcatctt ctggggcgga tacacctacc agtccctgaa
     4441 ggaaaaccag atcgcccagg tgggcgacca gttcatcagc atcaaggact acaacgacac
     4501 ctacaatcgc ctggtggaga actaccgacg gcaattgggt cccgccttca acgaagagat
     4561 gatgaagcgc ttcaacctga aacaacaggc cctggaccaa atgatcaacc ggatcctgat
     4621 tctccgggct tcaagagaaa tgggaatcgc cgtaacggaa cgggaaatcc aacagaaggt
     4681 cctcgaatat tcggtattcc aggccgacgg gaagttcgat caaaagcgct acgtgggcat
     4741 cctgcgccag aatcggctca ctcccgagat gttcgagcag caattggccg atgaaatcgt
     4801 gtggcagaag gtggaagcct tcatcaagag gcgagccctg gtcacggaag aagaggtgct
     4861 ggccgatttc cggttcaacc agacccgcgt tcaactggct tatgtcatca tcgatccccg
     4921 ggattacgaa tcccgggtga ccgtggacga cgcttccctc caggccttct accaggaaca
     4981 cagggaagag tacaaagacc ccgagaaacg gcaggtctcc tacgccgttc tgcccctgga
     5041 cgattttctt tccgccgtca aggtctccga agacgagatg cggcagtact acgaggatcg
     5101 accggacagt ttccggtacg aggaagaggt gagggcaagg cacatcctga tcgaggtgaa
     5161 gccggatgct tccgaagagg atatggccaa ggcccgggcc gaggcggaaa aggtcctggc
     5221 ccaggccagg aaaggggtag actttgcgga actggccaag aagcattcca aggacaccaa
     5281 ctcggccaaa aagggcggag acctcgggtt cttcacgcgg cagaagatgg tacgcgagtt
     5341 tgccgacgcc gcatttgccc tgaagccggg agagatcggc gatgtggtgc ggaccgagca
     5401 cggctttcac gtgatcaagg tggaagaggt gcgcccggag cgggtggaat cctttgaaga
     5461 ggcgtggggc cgcatcgagg aatccctgaa aaaggagaag gcccgggacc ttgccttccg
     5521 aaaggcccgg gacctttcgg acctggccta cgcccagaga gacatcaaga aagcggccca
     5581 gtccatggat ttggccggga agattcgaga ggaatctctg ggcgccaagg acgctcttcc
     5641 cggtctcgaa ccgcccagcc cggaaatcgt gagaaagatt tttgccgttc ccgacaaggg
     5701 aaccacggaa gccatggaat tcgtccgcgg tttcgtggtg gcccaggtgc tctcggtaac
     5761 cccccccgaa gtccttcccc tggagaaagt caaagaccga gtggaaaagg aacttcgggc
     5821 ccggaaggcc cgccaggcgg ccgagaagga cgcatcggaa atgctcgagg aagcgagaaa
     5881 actccacagc ctggaacagg cggccaagac ccggaaactg gagctcctgc gcacggagtc
     5941 gttctcccgg tccaatccgg acaagaagct caagcttcct cccgaatcca ccaacgcagt
     6001 gttccagctg gattcggacc gcccgttccc cgaggccccc ctggatctgc gaaaacgcgt
     6061 gttcgtctgc cagctcctgg aaaagaccgt cccgtcggaa attcccgaaa acgaacgact
     6121 gtccgtcgcc aagcgcatcc agatgcagaa gcaggccatg gtctggcagg cgtggatgga
     6181 agagcagcgg ggaagggcga aaatcgagca gttccgggaa ctataggctt gccggggaag
     6241 gggaggacgg cggcggtcct tgtcgggaaa aatccgggaa gacggcctca aagaccgtgt
     6301 ccgcctggtg ccgggtatgt tccgggtccc ggctgttgtc gatgaggaaa tgggcccgtg
     6361 cgcgtttgga ttcaatggga agctgcatgg ccagggtttc ccgcgcttcc tcgaggctga
     6421 cgccgtctcg ggccatgagt cgctcgacct ggaccgcggc gggcacgtac actaggatga
     6481 tgacgtcgaa gcgggaatgc aggtcggctt cgaaaagcag gggaatgtcg aagactacgg
     6541 gccgtcccgg ccgggattcg agggcctcgg cccagcgcgt ctccatccat tccatgatgg
     6601 cggggtgcag gatggagctc acctcctgcc tgcatacggg gtcccggatg attctctccc
     6661 tcagtgcccg gcgctcgagg ttgccccggg caccgaaata gaccgggtcc aggagggatc
     6721 gaagccgact ccagcaaggg gtcccgggag acacaacctc ccgggccgcc tggtcggcat
     6781 ccaacaggag cgccccccgg cgggcaaaca ttgccgccac ggtactcttc cccgtggcaa
     6841 ttccccccgt gagggccact ttgcgctttt cggacatccg agtcccgggt tccgaggccg
     6901 ctgcttgcgg taccgttgga tcccttggga aatcagtaga cgacatcccc taccggtcga
     6961 gtgaacgtgt attcgaaagg cttctggatg agctctttcc cgacccggtc gccgctattg
     7021 ctggtggcgg caaagggcat ggtcaccagg cttcccgcga gtccgattcc cagagccgcg
     7081 atccccaggg gtcgaagaat caacacgtcg gccaggatca gttcccccgg cgggtcccac
     7141 ttgacccttt catcgatttg cgcccccgtg gaagaggacc tttgaggctc gtccgcccgg
     7201 acgggcagaa caaagaacca cgcaaacagg ctcaccatca ccacccagca tatccctcgt
     7261 ttcatggctt tctcctccga cgatgccttt ttactaccaa ttcggcctgt ggtcccggtt
     7321 acatgacatc tttctcactc agttcgatgg ctttgtcaat ttcggccttc aactgcgggg
     7381 ggagccccgg gatgtcgacg ctcaagaaac ccctcacgat ggtggcgacg gcttcctcct
     7441 cgctgaggcc ccgagacatc agatagtgga tttcctcctg ggcgatcttg cccaccgccg
     7501 cctcgtggga catctcgacc ccgtcgaccc gcccctcgag ctcggggacc gcgtaggtga
     7561 ggccgccgtt gagcatgagg ccccggcatt cgaggtgggc cttgatctcc ggcacttcgc
     7621 cgatgaggtg tccccgggag atgatccttc ccccgttgct gatggttcgt gccacgatct
     7681 ccgctcgagt cccgcgcttc ttcaggaaaa tccgaccccc gacgtcatat tcggagccgg
     7741 gggtccccac gatgacgctg tagaagcggg caacggcgtt ttctcccacc aggtgggtgg
     7801 tggggtacat ctggagcgtc ttgaccggtt tcatgcaaat gtaattgttg aggaaaagcc
     7861 ccccttcctc cacctgtccc accgaccgcg gcctcaccgc catgtcttcg gcccaattgt
     7921 ggatcatggt gaagctcagc ttggcgttct ttttcacgaa aaattcggaa attcccacgt
     7981 gaatgccctt ggtgagatga ggggaggtgg aacagcccgt gatgatgtgc agctccgaat
     8041 cttcttcggc aatgatgatg ttgtggacgt tttgctgcag tccttcctta tcgatgtaaa
     8101 gacaggcctg gatgggatag atgctcttgg cccccggaag cgcccggatg acgtatccgt
     8161 tgtgaaggtt caactccgct tcggccgtga acttgtcggt gtccaccgat gcgagcttcc
     8221 aataatagtc ccaaatccag tccagctctt ccagggcctt ccggatgggg atgacctcga
     8281 tgccctcctg cttgctcttg cagtggacga ccgtcgtgtc cttctgaaag taggtccccg
     8341 cccggtcctg ctcggaaacg tccaccccgg acatgagcag cctctgccgg tcgccttcgg
     8401 gcagttccct caacttgtcg tcggtcaggt aggcatgggg gtccgaggtc ttgtcgaatt
     8461 gttccaggtc cacgtccgga ccgatggccg ccttcttgtg aagggccctt tccgctttga
     8521 ctttcagttc gtctcgatag tgcatcgcac acattcctcg tagccgattt cactgatgca
     8581 tcgaaggatt tcacgaggat tcgagctgca gctcaaaacc ccgttgtaga gtacttggcc
     8641 cttgtctacg tccacgtaat ccaggatatg tcccgtatgg gtgatgatca gtcccatctt
     8701 ggtccgctct tttttcacct gcagccggga cttgctttcg atggcgccgc attccttctg
     8761 caacaacctg gcgatggtat ggcccaccag ggagatgttc tcgagatcca cccccgattc
     8821 cggctcgtcg aacaacatca ggtccgggtt ctgagccagg agctgcagca gttcggatcg
     8881 tttgatttct cctcccgaga acccaaggtt gacgtctcgt tggagaaatt cctggaaatt
     8941 cacgctttcg gccagcctgt tcacatccag agcggtgttc ccggcgcaaa tgcgcaccat
     9001 ctgttcggtc ttgaggccgt ggatggtcgg gggcctctgg taggacatcc cgattcccag
     9061 tcgagccctt tcatcaatgg ggctgtgggt gatgtccact cccttgaaca cgatcttgcc
     9121 cccgatgacc ttatactggg gatatcccat gagagtcatg agaagcgaag tcttgccgga
     9181 accgttgggc ccaaacagca cgtgcgtttc gcccggtttg atctcgaggt cgatatgcct
     9241 gagaatcacc ttccctgcaa gttccacctg caggtcttcg acgaccaaca tcttctttct
     9301 ccctcgtgca tatgatacat aagaagacga tagaatctgc aacaggctga gaaatccctg
     9361 actggcgggc ataaccatcc gtcagttcat tttttataag ctgtcacgag acgttgtgca
     9421 agggaattcc catggttcca cctcgcggtt gccgccgttt tcaatcgagc atgaatggat
     9481 cggttaggag gagacgatcc gtattcggac attgctaatt gctgatggcc gaacgtcggg
     9541 atgaatgcac gaaaacgacg gggcccgata cccgtttttc ggaggcgaaa aggtgcgtgt
     9601 ggactccttg agacccaatc ctacgaatga tcgttcattg gacctggccg acttctgccg
     9661 tcggctcatg acccttccgg tcaaggcaag gctcgatgcg atcctggccc gagaagatgc
     9721 ggagaaggtg tttgccggtc tccccgtcca ggacttctac ttttccgtgg tcgagatcgg
     9781 gccggaagac tccctgcctt tcctcgccct ggccggggtg gaccagctga attacctgtt
     9841 cgatctccaa tggtggaaaa aggaccgggt ggacccggcc gccgccatcg agtggatcgg
     9901 tcgcctggcc cgggccggcg aggaaaagct cctggcctgg ctgtaccagg tggatttcga
     9961 gctgctggtg acccttttca aacgatggat taccgtggtt ccggcccccg aggacgaaga
    10021 ccccctcgag gttcgggacc gcttgcccct gcgcaccctc gacgaccagt acttctggga
    10081 atgccgatat ccccagtatg aagacctggt gcaaggggtc ctgagccttc tgttcgaggt
    10141 gcatcgaggc ttctaccagg agctgttgaa tgcagtttac tgggcttcga gtgtcgagat
    10201 ggaagaagag gcctatcgat ttcatcgagg gcggctggag gatcggggga ttccggacta
    10261 ctacgatgcc ctggaagtct accggtccat ccaacccgaa caattcgcag cagacaaggt
    10321 ggtcccctct ttccccccgg aggcggattc tccctccccg tcctttgctt tggcaatgct
    10381 gtctcccaaa gacctgccgg cccaatccct ggcgattttg gaagacgggc tccttttaga
    10441 caccctgcag atggaactgg cctccctggc cacccgggtc ctcgttgccg atcggcttcc
    10501 aacggattcc ttcgacagct tccggttttc ctcgggaaaa gtcggggcgt acgtgaacct
    10561 gggcctgcag ctcctgggcg ctcgggaccc ccggcaggcc ctgggcctgc tgaccaaaca
    10621 ccccctcgaa cacctgtttc gcctggccaa cgaccggata tcccggccgg cccgggcgat
    10681 gaggaaaatc cttcggagcg gccgtccctg tggaattcat tgcctggact ctccctggtt
    10741 ggaaacggcg gaactcctgt gcgaagaaac ccccaaagtc atgcgaaagg acccgaagaa
    10801 tcccggcgcc ttcaaggaag acttcttccg atttccccgg gacctcaagt cggcccggca
    10861 tgccgtggaa gtgatcgatg ccgtggggcc gctcctcgaa gctccgggca tggctcaacc
    10921 cgagctttgg gcaaagttgt gggggcgcgc tcaggtttca gacccccggg acatcacccc
    10981 ggcggtgatg gtctgcaccg ccgcaggaaa tgaaatccgg cggggggtct ggagcccgag
    11041 gcctttcccg gttcacgagt gggccggcat tttccccctc ctcgagccgg gccgcatccg
    11101 ggaggcgatc tcgagatgga ttcgcgaggt gctcaaggac ccgcgccaat gggagcgggc
    11161 cgaagcctat tttgctcccg tgctcgaaaa ctacgccctc gaaatggagt cctttggccc
    11221 gggcaacgcc ccggacccgg agatggtgcg gttctttctc ttcacggaag aagaataggt
    11281 ggagcagggg agggaaccgc cgggtacggg gaagaatgcg ctcatttggg cctaaggggc
    11341 gggccctttc ccgctgtccg cccttcccac cagtccgcgg ggggtcaggt tcgtgagctt
    11401 cacccggacg agggtcccgg gagggatggg tgcggaggaa ggaaaattca cgtgcaggta
    11461 gttttccgag gtcccgccgt accagccgct tgcgatctcc gtttccgcca tcacctcgac
    11521 ccaagtcccc agggcttctt ccatgaaggc aagcttcttg tgggccccca actcccggag
    11581 ggccctggcg cggtctctga gttccccccc cacgaccctg ccgggaagct tcgacgccgg
    11641 ggtccccggg cgcggagaat agggaaagac atgaaggtag gtcaagggga gcttgcggac
    11701 cagttcgtag gtttcctcga aggcccgggg ggtctctccg ggaaacccca ccatgacgtc
    11761 ggcgcccagg gacgcccggg gaaagaggga ccgcagttcc cgaaggattt ccccgtattc
    11821 ccggggggta tatttgcggt tcatgcgacg cagaatctcg ggatgcccgc tttgcagggg
    11881 aacatggaag tgggggcaga tccacgacct ggcacggatt tcccccagga cctctccgcg
    11941 aatttccagg acttcgaggg agctcaacct cacgcgcttc ggacgaaccg tctcgtccat
    12001 tcgacggaga agctcggaga agcgacgggg gggactgaga tctcgtcccc actgtcctag
    12061 gtggactccc gtgagcacca cttcccggaa gccttcctcg acgaagacgg ccgcctgggc
    12121 gaagacgtcc tcgaggggca gacttcggct tttcccccga gtgtacggaa caatgcagta
    12181 ggaacagaag gcatcgcagc cgtcctgggc cttgaggagt gcccgggcac gtcctcccgg
    12241 ggggcaccgg acgggaacgg ctcggaagga accgatggat cggggatcgc ccacggacac
    12301 gacgggatga tcccggcttg ccggttcgtc gagccgccgc aataggtcgt acttatcgtc
    12361 gtttcccagg atgtgggtgg ccaacccctc cctggcgatg cgacgtccct cgaggacggc
    12421 gttgcagcct gcagccacca cgagggcgga gggattggtg cgggcggccc tgcggagaag
    12481 ccggcatgcc tcgtgggatg ccttggacgt gacgccgcag ccgtgtacga ggtagacctc
    12541 ggcgggggtc gagaacggga cctctcggta cccttgattc gtcaggacct cgaggaagta
    12601 ggaggactca tactggttca ccttgcagcc cagggtttga acggccacgc gaagactcat
    12661 gagaaaaact cccgggacag cctcacgggg tcctgcaaat ggttccgccc cccagcagcc
    12721 ggtcgccgtc gtagaacacg gcggcctgac cgggagtgac ggctcgttgc ggttcccgga
    12781 accggacggc cacccgggca ttttcgaggg gagtcaatag ggcggctgca ggccggtgtt
    12841 ggtttcggat tcggacctgg caggagatgg gatgggacgg gcggtcccgt gaaacccaat
    12901 tgacccggtc ggccctcagt tcctgccggt agaggtcctg ggcccgaccg acggtgacgg
    12961 tgttggatgc ggggtccagg tccaccacgt aatagggggc gctggaagcg atgcccaggc
    13021 cccggcgctg acccacggtg taggcgaaga tccccttgtg ttctcccagg acccgcccgt
    13081 ccaggtccac gatgggacct cgatccccat aacgggaaag atccacccgt ttccgcacga
    13141 aatccaggta cgagcccgtt tgaatgaagc aaatctcctg gctttccggg ggaatttcgg
    13201 ggcggatgcc ctcctcctcg gcccaccgga acacctcgga cttgagaacg tcccccaggg
    13261 gaaacagggc ggctgccagc tgttcctggg tcaacccgtg caggaaatag gactggtcct
    13321 tcccggggtc ccgggccttt cgcaggcccc agcgcccgtt tcgctccccc ggagtcgcaa
    13381 tccgggcgta atgtcccgtg gccaggaact cggcccccaa ttgccgggcc ttcttcagaa
    13441 gaacgccgaa cttgatctgg ggattgcata ccacgcaggg gttgggagtc attccatcca
    13501 ggtaggcgga aagaaacgat gcgatcaccc gggcttcgaa atcgtcaacc aggtccacga
    13561 tccgaagagg aatgccgaag gcgcccgcaa gccgatacag gtcctcctcc gggctggaac
    13621 gcggggaagt tccgagcccc ttgcccgtcg gcccggaaac caggcgcatg tgcaggccga
    13681 tcagttcatg accttgttgc tttagcagga tcgccgctct cagactgtcg acacccccgc
    13741 tcattgctac tgcaacacgc gctcgtttca ccgtcttcct gctccaggtg ctcgtcctcc
    13801 agcttgacga ccttctcggg acggcccgtc ttgcgccagt aatcgttcac ggcggctttc
    13861 aaggcatcgg ttcccaggtt cgagcagtga agcttcttgg ggggcagccc acccagggcc
    13921 ttggcgacgt cgtcccggtt gatggcgacc gcttcatcca agctcttccc cttgaccatc
    13981 tcggtcacca tggaactcac ggcaatggcg gccccgcatc cgaaggtctt gaacttgatg
    14041 tctcggatgg tgtcgtcctc gaccttgatg gtcatcctca tgatgtcgcc gcagttgacg
    14101 ttgcccacct ggccgattcc gtcggggtta tccacctccc cgatgtttct ggggttcatg
    14161 aagtgctcaa ggactttctc actgtagttc atttccagac tccgctgctt tcatccggca
    14221 agatcccgtt ctccggctgc cgatcccgag aaagggcaac cgaagctagc tgaccagaac
    14281 tttgtcgaag gtcacgatca tggcccggga ggggcacacc ttcacgcaaa gctcgcacgc
    14341 gctgcatcgc tccgactcga acaagacttc catttcaggc ctcagaatgt gcagtgcccc
    14401 cgtgggacaa acggcggtgc aggccccgca ctggtaacat cggtcatcgt ctcttcgaat
    14461 gccttgggcc acgggttcga tctggacccc cacatccttg aggtattgga ttcccttttc
    14521 ataatcgctg cggagtccct gcaactcgag aacgacgatg ccttccttcc gcggatagat
    14581 ctgggccttc aggatattga aggtcagatt gtagtttcgc acaaggttgg tgataatggg
    14641 tttgtccacg atttctttgg aaaaacgcaa aaccagcatc ctggaataca tgggtgagac
    14701 tccttcgggt tgtcgagcaa atcgataaaa aataacaccc gggattcaaa agcccaaacg
    14761 ctttttggga aaaatgaagg ggtcctttgg gtccaagcgg ttcatttacg gagcaaatcg
    14821 gattatcttc tgggcaggct tcccgggaat ttgcggggtg ccgaacccgg ctcgattccg
    14881 ccccgttgga ggtcgcatgc agggggaacg ggccatccat ggacgggcag acaggtagaa
    14941 aggaaggttg ccatgcatga ggagccgttt cagtcccggc gggaccgatt acgaacattg
    15001 atgcgtcaac aggacctgga tgctttcctg gtggcggttc ccgagaaccg ctactacctc
    15061 agcggatacg gcgaagagga tctgcagctg acggaaagct cgggctacct cctatttgcc
    15121 tcccagggac agtacctgct cacggacttc cgctatgaag aagcggccaa ggcacaggct
    15181 cccggctttg atctggtgat ctacgaggag gggctgggaa agtcccttcc cgagcttttc
    15241 tccgacctcg aggtggattc cctggggatg gaaggacacc acctcaccta ccagcggtac
    15301 ctggaaatcc gggaggccct cgagaccgcc cgcccgaagg cccgcatcgt ttgccccgac
    15361 ggcctggtgg agagcctgcg aatcatcaag gacccctcgg agatcgagaa gatccgggcc
    15421 tcgctccggg tgacggaaga agtcctcaag gccgtgtggg aacggatccg gccgggcgtg
    15481 agggaggtgg acctcgcctg ggagatcgaa agctccattc gaatgcgcgg ggcccaggcc
    15541 gtgtccttcc cgcccatcgt cgccgggggg cccaacgcag ccctgccgca cgcggtgccc
    15601 accctgcggc ccatcgcccc gggagaaacg gtggtcctgg acctggggtc caagctggac
    15661 cattattgtt cggatatgac ccggacctgt ttcccgggca acccgggacc cggggtacgc
    15721 caaatctacc gcgtcgtgag ggaggcccaa ctggcagccc agagcgccat ccgggccggg
    15781 gtggattccg tggaggtgga tgcggttgcc cgaaaaatca ttggggacgc gggctacggg
    15841 gactttttcg gccacggcct gggacacggg gtgggacttg cggtccacga aaggccgggt
    15901 ctgaggaagc gcaatccgac ggttctcgag gaaaacatgg tggtcaccgt ggagccgggg
    15961 atctacctgc cgggctatgg tggagtgcgc ctggagaata tggtaagagt gacctccgag
    16021 ggttgcgaag tcctgaacga cgcggacctc ttctacgaat ttgcatgaat tttcccggga
    16081 cgatccgtgc ctcttgtcct tgaaaccggg cgaacccggc cgggacaagc ctccgagacg
    16141 ggacggcgcc ctttgaaggc ttccccctgc aaaggaagtc gggcggaggc cgcatccgcc
    16201 gggtcccccc cgggggtcat ggactccccc gggcatggga acggcgtaga acctttgcag
    16261 cgaggaacca accatgcaat ttcagaaagt cgccttcaaa aatgccgagg gccggatgct
    16321 gtccggccgt ctcgacctgc ccgtcgatga aatgccggac gcttatgcga tttttgccca
    16381 ttgctttacc tgcaccaaga acctcaacgc cgtggtgaac atcaaccggg ccctggcccg
    16441 ggaaggcttc ggagttctcc gatttgattt caccgggctg ggagaaagcg agggggaatt
    16501 tgccgagacc ggtttttcga acaacgtgga agatctggtt gccgcggcac gtttcctggc
    16561 ctccgatttc caggcccccc aactgctcat cggccactcc ctgggcgggg ccgccgtgtt
    16621 gcaggccgca tcccggattc cctccgtcaa ggccgtggcc accatagggg cgcccttcga
    16681 cctgacggac ctttccggcc acctgggaac ggcggaagaa acaatccggg agcggggaga
    16741 ggccgaagtc cacctggggg gcaggagctt caccatcaag cagcatttct tcgacgacct
    16801 tcgccgggcg aagattcccc aggtcctcga aaatctcgag aggcccctcc tggtgctcca
    16861 ttcggaagcg gacaacatcg tggggatcga ccatgccgtc cggcttttcc agacagcccg
    16921 gtttcccaag agcttcgtct ccctggaccg ggcggatcat ctcctgtctc agaaggcgga
    16981 ctctctctac gtgggttccg tccttgcttc ctgggcaagg aggtatatgg atccgccccg
    17041 gaaggaatca agatccaggg acttgaagga caatcgcatc gtggcccgga tcggcagaag
    17101 ggggtaccag acggagattc ttgccaacgg ccacagcctg gtggccgatg agcccattgc
    17161 cgtgggcggg gcggataccg gtcccagccc ctatgatttc ctggttgccg ccttgggggc
    17221 ctgtacggcc atgacccttc gcatgtacgc ggaccgcaag gactggcctc tcgggtccgc
    17281 cgtcgttcgg ttgaaacacc ggaagatcca cgcaaaggac tgcaaggaat gcgagtccaa
    17341 agacggcatg atggaccaga tcgaaaggga gatcgagctc gaaggccccc tggacgagaa
    17401 ccagcgcagt cgcctcctcg aaattgcgga ccgctgcccc gtgcatcgga cccttcattc
    17461 gcccattgcg gtgaagaccc ggctcaaaga ttgacgctcc tttcccgggg ccgaagcaaa
    17521 acgaaaggtt tatggaaatc atgcgttcta ttgccggtac gctggaaatc ttggtaattc
    17581 tggtcctcat cacggttaac ggcgtgttct ccatgtcgga gtttgcggtc atttccgccc
    17641 gcaaatcccg cctgcagcag ctggcggagg aagggagctc ctccgccgcg gccgccctcg
    17701 aactggccgg gtcccccaac cgctttcttt ccaccgtaca gatcggcatc accctcatcg
    17761 gagtcttcac cggcgtgttc agcggggcca ccgtggctgc cggcacggcc cggtggctct
    17821 ctcagttcga gcccctccgt cccttcagcg gaaccatcgc ttttgtcctg gtggtcttcc
    17881 tggttaccta cctgtccctg gttttcggag aactggttcc caagcgcctg gcgctatatc
    17941 acccggaaag gatcgcctcg gtcatggccc cgttcatgcg tttcctttcc atgatcgcca
    18001 atccgatcat tcagcttctc agcttttcca cggaaagcgt cctgaagctg gcgggcgtcc
    18061 gtccttccca ggagcctccc gtgacccagg aggagatcaa ggtgctcatc gagcagggga
    18121 ccgtggcggg agtgttcgaa gaggcggaga aagcgatggt gcagcgagtc ttccggctgg
    18181 gggaccgccg ggccggagcg ctcatgactc cccgcaagag ggtccagtgg ctggatgtga
    18241 acgaatcccc ggacaagatc cggcgccgca tttccaagag cagctactcc cggtttcccg
    18301 tgatccagag caaggccggg caggtcctgg gggtcgttca cgtgcgggac ctcctgatgc
    18361 ggagcctcca gggacaaccc ttcgatctgc gggcctgtat caagccgccg ttgtacgttc
    18421 ccgaagacat gcagctgttg aaggtgctgg aacggttcaa ggaatcgggg atggaaatgg
    18481 gcctggtcgt agacgaatac ggcacggtgg aaggcctgat taccctcaac gacctcctcg
    18541 aggccattgt cggcgagttc tcgtcccggg acaccccgga agagccccga gtggttcaaa
    18601 ggccggacgg gtcctggctg gtggacggaa tgctgcccat cgacgaattc aaggagctcg
    18661 ttcagatcaa gaaggagctc ttgggcgagc gggaaggcta ctaccagacc ctgggaggtt
    18721 tcgtcatgac ctacctgggc aggatccctc gagccgggga ccggttcgaa tgctgcgggg
    18781 tctccatcga gatcgtcgat atggacggac accgggtgga caagttgctg atcacccccg
    18841 gcggaaccct ggaagaaacc agggaagaat agaggccgat cgaggagatc cgttgggaac
    18901 ccgtggaaac aaaaccaagg cggaaggtgg tgccgaccat gttgaaactc tacaacaccc
    18961 tgtcacggaa gaaagaaacc tttcggccta tggaggatcg aagagtgcgg atgttcacct
    19021 gcggtccctc catctaccga agacctcacc tgggtaacta ccgcagcttt ctgtgggaag
    19081 acgtgctcca gaggtacctg gaattcaagg ggtcccgggt ggaccgcgtc ctcaatttca
    19141 cggacatcga ggacaaggcc atttccgagg catcgaggga ggggctgagc gttcgggaac
    19201 tgacggaagg aatcgcgaaa cggtttgtct ccgaggcccg gatgctgcga atcaagcttc
    19261 ccccggagat tccccggtct tccaccagca tcgatcatgc ggttcgcctc atccgggtcc
    19321 ttctggaaaa gggatatgcg tatcgccatg gaggggatat cttttttgac cctctccggt
    19381 gcgaaagctt cggacggctc tttggcgtgg acatgtcccg gtggccccgg gagaagaggc
    19441 gctttcgcaa ggacacctac ccggggagga gatggaacct cggggatttc atcctgtggc
    19501 acgggtccaa agcaggggac caaccgtcct gggatacgga gatcggcccc ggcaggcctt
    19561 cgtggaacat tcaggacccc gccatcatca cccagcacct gggattccag gtagacctgt
    19621 gctgtggggg agtggacaat ctgtaccggc accacgacta tacccttgcc gtgatggaag
    19681 ccgcttccgg acaggaattc gcccgctgct gggtccacgg cgaacatctg ctggtgaacg
    19741 gaaaaaagat gtccaagagc gtgggcaaca tcctgtaccc ggaggacctg ctggaaaacg
    19801 gcggcacccc cgaccatatc cggttctatc tcctctacgg acatcaccga aagagaatca
    19861 acctgacccg ggagcgtttc cgggagcgct gccgattgct cgatgacgta cggaacagga
    19921 tcggagaatt gatggcgaga gtgcctctga ggggcactcc gggcgggaag ccggggaagc
    19981 tgggcgcagg cctccttcat ggctttcaga aggccatgga cgatgacctg aacgtcctcg
    20041 aggcgacccg gtccgtcatc cggggcctgg gggaactcct gcgaatcagg gaccacgggg
    20101 acctcgacac ggaacagttc ctggacatcg tggccgccct gcacaggatc gattccgtcc
    20161 tgcagttcct gcttcccccc gagcaccacg aggacgaggg cccatgaggg cacccaaagg
    20221 gatcgtccag gttgccgggg tgttggatgc ccaggaagcc cgtatgctct tggactgcgg
    20281 ggcggacctc ctgggatttc ctctaaaaac ccccggaaac gatgaaatca ccgaggaccg
    20341 ggctagggag atcattcgct ccctgaacct cgagaaccgt tcggtcttga tcacctacct
    20401 gaaccgggcc gaaagcctcg cggacctctg cggcaggttg ggcgtaaaga tgctccaggt
    20461 ccattcggag atttcccccg aggaagcccg gcggttgagg aagacagccc cggatttgtt
    20521 catcatcaaa agcctcatcg ttgggaccga caatgtcgaa accctcgaag ccgcggtccg
    20581 cgatttcagc gattgggtgg atgctttcat cacggatacg ctggatcccc aaacgggggc
    20641 ccggggagcc acggggaacg tccacaactg ggccgtcagc cgccgcattg tggaacggtc
    20701 gccccgcccc gtgatcctgg cgggaggact ctgccccgaa aacgtcgccc gggcggtcgc
    20761 cgtggtgcgc ccttggggcg tggatgctca cacccggctg gaaggtcccc gggggagaaa
    20821 ggacccactc cgggttcaaa gctttgtccg ccgcgcccga gaagcccttt cacgggcgat
    20881 ggatcgggaa ggggaaacgg aaacggtccc cggccccatt cctgagacga tggaatccgg
    20941 aacggtgtag ggggccccca cagccgcagg ggagcggcag gcgggattcc ggtgggggag
    21001 tctcgggcat cgggggctac ccgccctgcc ccaggaaggt cattgcctcg attctcggca
    21061 ggatgggagc ataatcgggg tccgcatcgg gcagctcctt tcccggatat agggtcgtga
    21121 cggccgtgtg gccgacgacg gttcgtcctt ccctgccccg gaaatcccgg gttcgaattc
    21181 cccaaaaggt gtgaaatacc aggatttctc cctcgtagga tcccaaatag agcatgatgt
    21241 gtccccgggc ccaaaggagc gtgaagaaag gtacggcccg ctgttttacg accgtctcct
    21301 tttctttggg cgaaaggccg cttacatcct cgtagagccc gccccggctt gcctggtgag
    21361 tcgagttgcg ggggagccag aggccgaagg gggtgaacag gtctttgagg gtggccgagc
    21421 aatcccggtt gaagtaaagc cctccccacc cgtagggttc tcccgcgagt tcgtcggcca
    21481 ggcgggcaat atttcgggcg gtgagctcga ggggttgaag gacggcggca tcttggggaa
    21541 gggtggtgga gaccgggacg gcgtttcgcc gagaatcggc gacggcgacc caaatgcggg
    21601 tcccgtccgc ccccccgtct tgggcgggaa acagggaccc gatgtccgcc tgaaacagga
    21661 agccgccttg ggggtggtgg acgggcacac ggtcccgcac cagggccata cgtttgcggc
    21721 ctctccattc ccggacgaat tccgaatcca ccgccgccac gtctcgggaa tccatccagc
    21781 cggaactgta gtgcgaatcg acgtagcacc aagccccgtc cttgctggtg tgagagatga
    21841 acacgggggt gttggcgggg atgctcgagt tctgcagaat gtcgaaggga tagccttctc
    21901 ccggttggtt cgggtcggca aaaagcggcc tcagggtggg gagcatccgc aggtgggaac
    21961 gatccacggt gatgcctctc cggcatgtgt tgggatagcc gtcgaggtcg gcgtttgccc
    22021 ggatgacggc cgcccactga tgagtgtgcc tccggtagtt ctctccgtat ccgggatctt
    22081 ccgtgtattt cccgacctgt gccgtgacct gttgccgggt gacctggggc cctccccggt
    22141 cccagggcag aaaaaaggcc cggtcatatt cttccatgag gacttcttga cgagacctcg
    22201 tgaccagggg acggtcggcg gtccgggccg aaaggtagtg ggaaggtttc tggggcaggc
    22261 ggcggatgtc cgccagggag tcgggatgat accggcatcc ggccaaaagg accaaaaagg
    22321 cggccaggac ccatggggac cggacactgc ccgttgaggt tttcatgcag ggggctccgc
    22381 cctttccgtc cggtccggca aaaccggttt ctgccgcgga ccttttgccc cgggggcaga
    22441 ggttcgcgga tcggaccccg tgatctttgc gttggccata cggcgggtcc gccggacctc
    22501 gcggattttc tcccggcgtt tctggtcccc gtattttccg aagcgtccgc ttttcatggc
    22561 ttttcgtcct ccatgggtac g
//
LOCUS       DAOOVC010000055        22269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1594_length_22269_cov_6.354776, whole genome shotgun sequence.
ACCESSION   DAOOVC010000055 DAOOVC010000000
VERSION     DAOOVC010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 22269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22269
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_22269_cov_6.354776"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(474..875)
                     /locus_tag="PLM79_08960"
     CDS             complement(474..875)
                     /locus_tag="PLM79_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV86478.1"
                     /translation="MFPKIEIESLPVTDSFVRRKRVIQPRGELALIEDGGNFRHLGYF
                     SLKSGPGLFRGGHYHRKKTEHFYVVGGELLLRLVDVDTGEKAVLRAEPGQVIRLHPGC
                     AHRFEAVRDAQVIEYYDGVYDREDDVAWQDF"
     gene            1187..3394
                     /locus_tag="PLM79_08965"
     CDS             1187..3394
                     /locus_tag="PLM79_08965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOV86479.1"
                     /translation="MLKALAVMTSGGDSPGMNAAVRAVVRRALDYGIQVYGIRFGYEG
                     LVTGGDQIVPLQWKDVGGILQKGGTFLGTARSDRFRTPEGRRQATLHLLEREIEALVV
                     IGGDGSLSGAHLLAREWSEHVEGLGRSSPAGEVHPLQVIGLPGSIDNDLHGTDMSIGA
                     DTALNHIVRAMDDLSSTAASHQRTFVLETMGRHCGYLALTAALAGGASWVLVPEEELE
                     LRWHQKMVAALDHAREIGRTHQMVVVAEGARHPDGLFINSNEIRKLLVDRLGIDVRVT
                     VLGHVQRGGSPTAFDRVLATRLGVAAVDTLMQGPEGVHCHMVGLVRNTVVKTPLSEVV
                     EKSRAVGMEIENGDYARALSLRGGSFRNALDLVKTLTRISPAQETRDDGIVVILTAGA
                     DAPGMNTAVSVAARCLLNEGIRTYASPDGFLGLIRGNLQPLDWHQLVGWVNRPGSEIG
                     TARYEFREGDLERIAGTVETNRVRAIVAIGGWGTYRRAAQLAESQDRLPALRIPVVLI
                     PATIDNNLPCTEYSIGTDTALNNIVEAVDKIRHTAGASRRAFLVEVMGRRSGFLALMG
                     ALASGAEKAYLPELGIRLSELNQDLEHLRTSFRQGKRLVILLRNEHSSARYTTDFIRS
                     LFEEESEGAFQVRTAILGHIQRGGIPTAFDRILASRMGAGAAGSILESLERGSARCTV
                     VGLSGRGIREFPLEEALEQMEMGEGRPKRQWYMDLVEMAQALAKYGPSRSSSS"
     gene            complement(3712..5238)
                     /locus_tag="PLM79_08970"
     CDS             complement(3712..5238)
                     /locus_tag="PLM79_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOV86480.1"
                     /translation="MGKRGIWILCVLFLAGISWTPLTARGSTGRINCRGAILVDAANG
                     RVLYEQNADALIPPASITKVLTLYIVFEAIRQGRLDSSDLVYISQRAAGAPPTRMGLK
                     AGRTVVLDELIKGMAVVSGNDACIAVAEHMSGSVEAFVAKMNAKARELGMTSSHFKNP
                     NGLPAQGQVTTARDISKLSLAYLRRFPNSLNIHSMQSYTYNSSTRRNANRLLGTCYGV
                     DGIKTGFVCASGYNLSATAKRGDVRLIAVVLGAPSPGIRARETAKLLDAGFDFIEAKT
                     PEIRLVEDPKGGRSGRITVAKGASGPTHGVNKKKGKTQQQASVKKTSKKRLATSAGKE
                     APVSSGKKGKTASLAAKKVSAEPRAVKAVGASKKEGTVARARVEKSAPAVKGTAATAG
                     AVSKRAASPGKPAENKSAKSAKSSKEAKTSEAAKTVKTSKTSTTAKTAKKENSPSKNA
                     AAKTASKPKAGREKEKAVAGRGNGKTKGKSGQTAETPKKGGVVQKSHKDPNPSKPKSG
                     "
     gene            5761..6783
                     /locus_tag="PLM79_08975"
     CDS             5761..6783
                     /locus_tag="PLM79_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV86481.1"
                     /translation="MMGRMLDFLSKDMAMDLGTANTLIYVRGKGVVLDEPSVVAMEVG
                     TSKVLAVGKDAKNLTGRHGRNVQTLRPMKDGVIHDFEAATYMIRAFLGKVFKRLPLSR
                     PKLVVAVPTGITAVEKRAVMEASEMAGAGKVFLMEEPMAAAIGTGLPVDQSVGHMVVD
                     IGGGTTEVAIISKFTVACSESLRIAGDEANEAICRFVKDEHRMSISDTMAEVIKIKIG
                     SAVPLKRQLEVKLRGKDISTGMPKVVSITDADIRDAIKMPTMAIVEAVRRVLEKASPD
                     FASDIGENGIWLAGGGALLKGLRLLLHQATGLDVKKSEDPLRAVIRGTGAVTERFDFY
                     RDVFLS"
     gene            6978..8705
                     /locus_tag="PLM79_08980"
     CDS             6978..8705
                     /locus_tag="PLM79_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HOV86482.1"
                     /translation="MIIGLDVGGTHTDVVLLGKDRILQQAKVPTRPSSLLESVWSGIE
                     EVTRGIAPEAIERVVLSTTLTTNAIAEGKLAETGMIVSSGPGIDPEGFRTCREFHCVR
                     GSIDHRGREIQPIDRAQVESAADGMRSRGVRYVGVVGKFSIRNPKHENEIVRILGDRF
                     EHVVLGHRLSGNLNFPRRVCTAYLNAAVYPVYKNFFEAVSASLKKKGLRIPIYILKAD
                     GGTVTLEASLSSPGQTILSGPAASVMGSVPYASREEETLVLDIGGTTTDMAVLVRGVP
                     LLDPVGIELGGYKTLIRSLKTHSVAIGGDSAVRLEDGRIQVGPDRQGPAMAYGGTVPT
                     PTDALAVMGKAAGGDSERARQGLLPLAQQLGVPLEEAAARIFDHTCKQILEAAAQMVA
                     NINRKPVYTVQELLEGHQVRPTEILVLGGPAPLFAFRFAAMTDFRVRVVPRWQVANAV
                     GAALARSTCEVTLFVDTERGFAVAPEESFHLSVRRDFDKQSAVRLALELLRKKCTQEG
                     SADDGTEMELVEELQFNMVRGFYTTGRNIRVKAQVKPGLIPEHAQIAAGLFHAGPTGG
                     QDHEEDSHG"
     gene            8698..10011
                     /locus_tag="PLM79_08985"
     CDS             8698..10011
                     /locus_tag="PLM79_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOV86483.1"
                     /translation="MVETPSKVGLVFFPAYDWAISPTHPEREERLLYTQDQVFEEGIL
                     DLEGIVELKPDLVDVRDIRRVHFCVPEEGRVVTESHLISAGGAKTVGIEVLEKRVRRG
                     FALVRPPGHHAMRVVHGARGFCNINIEAIMIEYLREAYGVDRVAVVDTDCHHGDGTQD
                     IYWHDRDTLFISIHQDGRTLYPGSGFHTEMGGPNALGTTVNIPLPPRTSSEGFLFVLD
                     EVILPLLEEFKPEIVVNSAGQDNHYTDPITNMCFSAQGYAELTRRLKPDIAVLEGGYS
                     IEGALPYVNVGIIMALAGLDTSHVKEPDYDPNKIRQSAATTRYIESLVKEIRETRRRM
                     AGGGKARSGDSTAQRRRNIFYDTDQILEAQEETLRLCDRCNGVLGIDSRTDRGNHIYA
                     VMIPRKACKECCELGYEWFDSADHRKYNITFLQDRTQDQYLVRKA"
     gene            10017..11111
                     /gene="rlmN"
                     /locus_tag="PLM79_08990"
     CDS             10017..11111
                     /gene="rlmN"
                     /locus_tag="PLM79_08990"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOV86484.1"
                     /translation="MKTDAILIKNFTLQQLEDWVESVGERRFRARQLYRHLYVRRIRS
                     WDECSDLSKSFQAQLEFGTVLDALSLEEKRTSSDGTVKYLFGLSDGRRIESVLIPDPP
                     RYTLCLSSQVGCALSCRFCLTGTVGFQRNLSAAEIVDQVCRVQQEAGNRFRISNLVFM
                     GMGEPLANYEAVSNAVHILCDPNGMAFSHRRITVSTAGLVPQMERLGRDSPVNLAVSL
                     HAPEDGLRSELMPVNRTYSLGDLLNACRRYPLAPRKRITFEYLLLDGVNDQPRHAKAL
                     VKLLHGVKAKVNLIPFNSYPGSEFRRPSEERILAFQEILTGAQLTALVRQSRGFEIGA
                     ACGQLAALGKDDPSSRGETEAGHGFFSLPG"
     gene            11221..11565
                     /gene="hisI"
                     /locus_tag="PLM79_08995"
     CDS             11221..11565
                     /gene="hisI"
                     /locus_tag="PLM79_08995"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOV86485.1"
                     /translation="MLPVIVQDVKSGRVLMLAYMNELAWDKTIETGRAHYWSRSRQKL
                     WLKGEESGHFQVVRETYLDCDLDTILLKVEQIGGAACHTGFQSCFYRRLEGAEVIVSE
                     EPVFNPKEVYRK"
     gene            11562..12434
                     /gene="hisG"
                     /locus_tag="PLM79_09000"
     CDS             11562..12434
                     /gene="hisG"
                     /locus_tag="PLM79_09000"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOV86486.1"
                     /translation="MNQLVLGIPKGSLQEATIRLFRQSGWRISLSERNYFPEINDPEI
                     NCAICRAQEMSRYVENGTFDVGLTGRDWIMENDSDVEVIADLIYSKVSQRPARWVLAV
                     PFESPVQGLRDLEGKKIATELVNFTRRYFSSAGVNVQVEFSWGATEAKVISGLCDAIV
                     EVTETGTTMRANGLRIVKELMQSNTQIIANRNAIEDPWKREKIEQISMLLQGALRAEE
                     LAGLKMNVPEEKLNQVIEILPSLNAPTIAHLYRSDWLSVETVITKSKVRDLIPQLIKR
                     GAEGIIEYSLNKVI"
     gene            12434..13855
                     /locus_tag="PLM79_09005"
     CDS             12434..13855
                     /locus_tag="PLM79_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV86487.1"
                     /translation="MRLRRGFRVLCVAALLVSGGCATFKQEPSSKYTAEQLKEMGEKF
                     LTAGALPQALRLLTMAEAKRPKDPVIQYDLATAYDQRGLSSEALAHYQKALALKPDFP
                     EALNALGAYYAARGQYDLAEPCFQKALANPFYETPHLALYNLGLVYEKKGDLETALKQ
                     YQQAVRFQPAYGPAYYRMGEILEVLRRGDEAREAYGKTITYNPDMVEAHFRYGVMSYM
                     AGELENAFYSLSKVVRLAPHSSMATDSKKYLEQLQTLTVDRRKPPTVPTSEKLAHLEV
                     MAEQEVMQQQTRALPPPPISESTLSATPGTPAPTTSTPAVTPAPVAAAEATSPSGTGT
                     PGTGEEAKHPKKSAASPVSTPPAASPAAPSPAPAPESPPAAVKSPKPSGKISPSDMEW
                     TYIVQVGSFLDKENAEILLRRLKDKGYDAVLKNFNHQFLGLVYVVQLKPVDEVAKAST
                     LMAQVENEEQQVKPIIIKVPSNI"
     gene            13931..15124
                     /locus_tag="PLM79_09010"
     CDS             13931..15124
                     /locus_tag="PLM79_09010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOV86488.1"
                     /translation="MFMRKDSGEKGEKTLICKRVRGIKPFIVMDVLERAQELERAGEN
                     VIHLEIGEPDFETPEAVKNAAVEALRRGETRYTHSLGTRKLREAICEYYERQYGITKL
                     DPDQVLITSGSSPAFLVGMGTLLGPGDEVILTDPHYACYPNFIQFLDGTPRSIPVLEE
                     DGFQYRTEAIRKALTPKTKAILINSPANPTGNLLDADRMAEIAQLGPMVFSDEIYHGL
                     VYEGRAHSVLEFTDNCIVFNGFSKQFAMTGWRLGYLIVPRPLVRTMQKVLQNFFISAN
                     SVAQAAGYTALTDPSVEADVQRMRETYNRRRKFILRRLREIGFGISVEPTGAFYAFAN
                     AKRFTTDSYSFAFEILEKAKVGITPGVDFGPGGEGYVRFSYANSMANITEGLRRIEEY
                     LRNRS"
     gene            15134..15739
                     /gene="yihA"
                     /locus_tag="PLM79_09015"
     CDS             15134..15739
                     /gene="yihA"
                     /locus_tag="PLM79_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOV86489.1"
                     /translation="MENALRILSAEFATSALRIDQCPPPDLPEVAFAGRSNVGKSSLI
                     NCLLLRKKLVRTSRTPGQTRMLNFFRINGAFFFVDLPGYGFAKVSQEIRARWGPMMEQ
                     YLTGRSSLRGVVVIMDARHPATPDDLALWKWLRDRNIPGAAVLTKIDKLSRNKRPSHV
                     QQASLALGVPPDRLVLFSAVTQEGREAVLNLLESWIAPSQG"
     gene            15797..16717
                     /locus_tag="PLM79_09020"
     CDS             15797..16717
                     /locus_tag="PLM79_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV86490.1"
                     /translation="MPSKDRWLRIIDRFMKAAGGIPREWTRTPARAAGLLWYWTDRRH
                     RTVAHKNLSIAFGGTLDERARRRLCRESFQHLVQVFFELPYVYCLNASDAGRFITFQD
                     MEHLYRAMEKKRGVLVLASHFGNWELMSIGFSLLQFPIHVIARPIENPLVDAFVTRLR
                     SIGGTEVISKKGSFREVLRVLNRGRVVAILLDQNAAAHEGVFVPFFGKTACTHKAMAI
                     LALRTGAPVVPVYNFREPGGHYRMVFLPEIPLRVTGDMERDIRENTSLYNSTMEGYIR
                     LYPEQWFWVHRRWKTQPGVSRARQKAEYGV"
     gene            16800..17900
                     /gene="waaC"
                     /locus_tag="PLM79_09025"
     CDS             16800..17900
                     /gene="waaC"
                     /locus_tag="PLM79_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HOV86491.1"
                     /translation="MDKPLKILIVKVSSLGDVVHTLPVLYYLKSAHPGLTLDWIVEEP
                     FSQILVNHPLLHRLHLLHTKRWRKHPFESSGLREVLRLHRAVRKERYDVVLDLQGNTK
                     SGAVTLLTGAPSRFGFPASHVREWPNLLATNRKVRPVSELLHITDRNLAVAQAAFPGG
                     SPPPPSGYLFAEESRHREVDEYFERHRLGDRPTAVFLYGASWKTKLWDRDHWKRLVAS
                     TVKDLGMDVLLTWASEEERAACIDIRGSWDGRVLLWPRSHLKTVLALLEKAHVVVGGD
                     TGLIHMAAAAGTPTVSLYFVTSGRRNGPRGPLHRCLQSSMPCSPCLKKGCGHRVACGR
                     SIPVSAVLSAIGEVLDIGSSGGSRGSTPGTGT"
     gene            17908..18741
                     /locus_tag="PLM79_09030"
     CDS             17908..18741
                     /locus_tag="PLM79_09030"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOV86492.1"
                     /translation="MIFPMVQEIRFETDEALFRQSGFETYAHFMAPDTGTHVARKRGR
                     VVRRLDLEGRSFFLKRNRFHWIEFFKGLLHMRFLPRNGLREWRNIDLVTRAGIPTVRR
                     VAWGERRCLSREISSFTITEKLYGARSLKEILQERAAGKADAGSFREKRDLIQRVARL
                     ASRLHRHGLYHQDFYLGHIYKGPDEVLYLIDLQRVLCSPLASRRYRIKDLAQIHYSAM
                     EIPGISASDRLRFLLAYTGSQRVGREEREMIQKILAKTGRIARHTVKLLERRRRRKEI
                     P"
     gene            18738..19700
                     /locus_tag="PLM79_09035"
     CDS             18738..19700
                     /locus_tag="PLM79_09035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV86493.1"
                     /translation="MTLLSVLIVNWNTRDDLRECLRSIGENLTSLRPQVIVVDNGSRD
                     GSAAMVSCEFPAVTLLSSPTNLGFARANNRALQQASGDFLLLLNPDTRVGKGAIEGLI
                     ETMAADPAVGIAGLQLLNSDGSKQNSISSDPSLLTELTNKSLLRMLFPRKYPGKELEL
                     TRPIDVDAVIGACMLVRRKAFEEVGPLDEDYFLFLEETDWCRRMRRKGWKVVHDPRFT
                     VLHLQGKSAGQVPTAARTEYWKSRYRFFQKHYGPGVHATLKAGLILKLAGSCLVNTVL
                     GLATGFLHSKTRQRLFLNLRLLEWHLRGCPPDWGLRPPQSSHSL"
     gene            complement(19697..21748)
                     /locus_tag="PLM79_09040"
     CDS             complement(19697..21748)
                     /locus_tag="PLM79_09040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV86494.1"
                     /translation="MNRTKQVPSGQGSPEGPGSFFHAPSFVGFEGPPPFRKIRWLSFA
                     GVMLLALFLFGFKMEDRGIWSSHEAIAGQIARAMVRSGDYGVERFETGRGYNNEKPPL
                     YYWGVSLFSRMTGEVNNLTIRLPSTVSAVLLVALVFWMGARMFSLPVGVMASLILMTS
                     IHYRWLARVGRIDMVLALLVIASGFALYFACRGERSRRSWILCAAAYGLMGLGVLAKG
                     PVGIVLVFCGIGPILLLEKRWEVIGELCLAWIAGALVLAGLCLGRQGWPVLVPLGGLV
                     LGGLFRKRIAVRPFSPHLLGVAVLLLVASPWFFWVHHETQGQFTRDFFIYHNFVRFFP
                     QGVAGYEALKHRPFWFYIPHLLTSMLPWSLLLTALPRKVLKRDRPSYDLRRFLVVWGG
                     VSFLFLSLSSFKRADYLLPVLPLLSLLLAWCLANFYEEDPDGKGIRTALTATGILLAT
                     ATAVLWLLALLTAFGDSLSSPVQTLVEHPAFESRFNSDDANGLLTYLRFFQAPENRVS
                     ILLFSVLMTAGVAGIFLKRRSLKWAFSLLVVLAAAFHTVHEVKIAPFFEPFRTQRTLA
                     AQIQEIVGKEGKLLMVETEPHDLVFYLDREVLWFPHVYPDRSLARVLAEEAGKPLYFI
                     LDRDRYRRMKKAGFLPGEVVAESIPEHFDGIVVLRPMPADSPDRFRQNPGPAAPVRES
                     G"
     gene            21978..>22269
                     /locus_tag="PLM79_09045"
     CDS             21978..>22269
                     /locus_tag="PLM79_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86495.1"
                     /translation="MRVLFLVPRLDKASTRYRVLQYLPYLQEQGVSCRVRTLSKDRGW
                     DRSLFREARSSDVVFLQKRILSPFLLLALRKASSRLVYDLDDAVMFRDEAATE"
BASE COUNT         4511 a         6783 c         6739 g         4236 t
ORIGIN      
        1 caagatgcac aagtgcacaa aagaaatttt gcacatggac tataccatag aatagatacc
       61 atggtcagta ccttttttac gaattttttt tgatgtgatc gaacttccaa tccccccacc
      121 tgcccgccag aaggtccgtc gagacctcaa aagccgccga tcctcggcct caaatcccgt
      181 tcgatttcct caaacccgaa ccaaaaaaaa tcgggcgcaa ggcccctccc cgggaatttt
      241 gtctcccccc ggggcaaaac ctcacaagta taacccgaaa actgcccatg gaaaccaccc
      301 agaggccccg gcaccggaaa aagtcgcccc ttgcgcaccg gctcccccca aggatcaacc
      361 cgccccattc ttggcgacac ccctcgcccc cggcatcccc tctgcaggac ccgttcgccc
      421 ttttgtgcaa tttcaccaac cggaccttcc cggtcgccgg cggcaaaccg gatttagaaa
      481 tcctgccagg ccacatcatc ctcccggtcg tagaccccgt cgtagtattc gatcacttgt
      541 gcatcccgga cggcttcgaa gcgatgagcg cagccgggat gaagacggat cacctggccc
      601 ggttcggccc gaagaaccgc cttttccccc gtgtccacgt ccaccaggcg aagcagcaac
      661 tctccgccga ccacgtagaa gtgttccgtt ttcttccggt ggtaatggcc gccccgaaac
      721 agcccgggtc cgctcttcaa cgaaaaatag cccaggtgtc ggaagttgcc gccgtcctcg
      781 atgagggcca gctctccccg gggctgaatc acgcgctttc tccggacgaa ggaatcggta
      841 acaggcaagc tctcgatttc gatcttcgga aacatcacaa ctccttggca ggatacgggt
      901 ctacaggttc ccggcatgtc cccgtcttcg ggaccggggg cttgaccttc ttctattgcc
      961 cataccccat gggatctgtc aaacattaag gagttgagat ggggggtttt tgctgacata
     1021 ttgaaggccg acggatcctg ccgggcaggt cgaaaccctc ccgccccccc cttgacgagg
     1081 ggcgcgggat tcacgggcaa agcgtgccgg aacgacaacc ccacgattcc gatccgccaa
     1141 ggaggcggcc ctgcaggaag gcaaacggat tttgaggagg cttcccatgt tgaaagcact
     1201 cgcagtcatg acgagcggag gggattcccc gggcatgaat gccgcagtga gggccgtagt
     1261 gcgcagggca ctggactacg gcattcaagt ctacggcatc cgtttcggct atgaaggcct
     1321 ggtaacggga ggagaccaga tcgttcccct ccaatggaaa gacgtggggg gaattctcca
     1381 gaagggaggc acattcctgg gaacggcccg ttcggaccgg tttcggaccc cggagggcag
     1441 gcggcaggca actcttcatc tcctggaacg agagatcgag gccctggtgg tgatcggggg
     1501 ggacggatct ctttcggggg ctcacctgct ggcccgggaa tggtccgaac acgtggaggg
     1561 cctggggcgg tcgagccctg ccggggaggt gcatcccctg caggtgatcg gcctgccggg
     1621 ttccatcgac aacgatcttc acggcacgga catgtccatc ggggccgata cggccctcaa
     1681 ccacatcgtg cgtgccatgg acgatttgag ctccaccgcg gcctcccacc agcgcacttt
     1741 cgtccttgaa accatgggac gccattgcgg atacctggcc ctgacggcgg ccctggccgg
     1801 cggggcatcc tgggtgctgg ttcccgagga ggaactcgaa ctgcgatggc accagaagat
     1861 ggtggcggcc ctggaccacg cccgggaaat cggccggacc caccagatgg tcgtcgtggc
     1921 cgaaggcgcc cggcatcccg acggcctgtt catcaattcc aacgaaatcc gcaagcttct
     1981 ggtggaccga ctgggcatcg acgttcgagt cacggtcctg ggacatgtgc agcggggagg
     2041 ctcccccacg gccttcgacc gcgtccttgc cacgcgcctg ggagtggcgg ccgtggacac
     2101 cctcatgcag gggcccgaag gggtccattg ccacatggtg ggcctggtcc gcaacaccgt
     2161 tgtaaaaact cctctttcag aggtggtgga aaagagcagg gccgtgggca tggagatcga
     2221 gaacggcgat tacgccagag ccctgagcct gcgggggggg agcttccgaa acgcccttga
     2281 tctggtgaaa accctgaccc ggatctctcc cgcccaggag acccgggatg acggcatcgt
     2341 cgtgatcctc accgccggcg ccgacgctcc cggcatgaac accgccgtca gcgttgcggc
     2401 ccgctgcctc ctcaacgagg ggatccggac ctatgcgtcc ccggacgggt ttctcgggct
     2461 catccgcggg aacctccagc ctttggattg gcaccagctc gtcggatggg tcaaccgccc
     2521 cggctcggaa atcggaacgg cccgctacga gttccgggaa ggggatttgg aaaggatcgc
     2581 cgggaccgtt gaaaccaacc gagtccgggc cattgtcgcc attggaggct ggggcacgta
     2641 ccgaagagcg gcccaattgg cggagagtca agaccggttg ccggccctgc ggatccccgt
     2701 ggttctcatt ccggcgacca tcgacaacaa ccttccctgt acggagtatt ccatcggaac
     2761 cgacacggcg ttgaacaaca tcgtggaggc cgtggacaag atccggcaca cggcgggggc
     2821 cagtcgaagg gccttcctgg tggaagtcat gggacggcga agcggattcc tggccctcat
     2881 gggggccctg gcctcgggag cggagaaggc ctaccttccg gagttgggga tccgtttgtc
     2941 ggaactcaac caggacctgg aacatcttcg aaccagtttt cgccagggga agaggctggt
     3001 cattttgctc cgaaacgagc attcctccgc acgttatacc accgatttca tccgcagcct
     3061 gttcgaagaa gaaagcgagg gggcctttca ggttcgtacg gccatcctgg ggcacatcca
     3121 gcggggggga atcccgacgg cctttgaccg cattcttgcc agccgcatgg gggccggggc
     3181 ggcggggagc atcctggaat ccctagaacg ggggagcgct cgatgcacgg ttgtgggatt
     3241 gtccggccgg ggcatccggg agtttcccct ggaggaagcc ctggaacaga tggagatggg
     3301 ggaaggaaga cccaaacgcc agtggtatat ggatctcgtg gagatggcgc aggcgctggc
     3361 caagtacggg ccctcccggt cctcgagttc ctgaacgcgg agcggacttg gaatttgggg
     3421 gccggagacg actgcgaacc gccccggcgc aagtcggggt tctccagccg gaccatgcgg
     3481 tcctctcgcc ggttcaaaca acctttgtcc tcacgagacg cgcccgcaca aagaccttcc
     3541 ggccgggtcg ggcagcggca ttcggggacg tcgaatccct ttccgttcaa aaaggaggcc
     3601 cctggggacg gacagctgcg gagaaggtgg cctcgggggg cgccaagggc gggaaagccc
     3661 cccggggaaa aggtttgaac ctgccccata accctcgttg ggtccgacgg gctacccgga
     3721 ctttggtttg gagggattcg ggtccttgtg agacttttgc accacccccc ccttcttggg
     3781 ggtttcggcc gtttggcccg acttgccttt ggtcttgccg ttgccccgac cggcgacggc
     3841 tttttccttc tcccgtcccg ctttgggctt tgaggcggtc ttggctgcgg cgttcttcga
     3901 aggggaattc tctttcttgg cggtcttggc agtcgtggat gtcttggagg tttttacggt
     3961 cttggcggct tcggaggtct tggcttcctt ggaagacttg gcgctcttgg cggacttatt
     4021 ctcggccggt ttccccgggg aggccgcccg tttggagacg gccccggccg tggcggcggt
     4081 tcccttgacc gccggggccg atttctcgac ccgagctcgg gcgacggttc cttccttctt
     4141 ggatgcacca acggccttga ctgcccgagg ttccgcggaa accttcttgg ccgcgaggga
     4201 agcggtcttc cccttcttgc cggaagaaac cggcgcctcc ttccccgcgg aggtcgccag
     4261 tcgcttcttc gaagtctttt tgacggatgc ctgctgttgc gtttttccct tcttcttgtt
     4321 caccccgtga gtggggcccg aagcgccctt ggcgacagtg atcctgccgg atcttcctcc
     4381 tttgggatct tccaccagcc ggatctccgg ggttttggcc tcgatgaagt caaatcctgc
     4441 atccagcagc tttgccgttt cccgggcacg gattccggga ctgggagctc ccaggaccac
     4501 ggcgatgagg cgcacgtctc ctcgcttggc cgtggcggac aggttgtagc ccgaggcaca
     4561 cacaaacccc gtcttgatcc cgtctacgcc gtagcaggtc ccgaggagcc ggttggcgtt
     4621 tcgacgggtg ctgctgttgt aagtgtagga ctgcatggag tggatgttga gggaattggg
     4681 gaagcgacga aggtaagcca gggaaagctt ggagatgtcc ctggcggtgg tgacctgccc
     4741 ttgggcgggc aaaccgttgg ggttcttgaa atgggagctg gtcatgccca gttcccgggc
     4801 cttggcgttc attttagcca cgaaggcctc caccgacccg ctcatatgct cggcaacggc
     4861 gatgcaggca tcgttgcccg aaaccacggc catgcctttg atgagctcat ccaggactac
     4921 ggttcggcct gctttcaatc ccatgcgggt gggcggggcc ccggccgccc tctggctgat
     4981 gtagaccagg tccgaagaat ccaggcggcc ttggcgaatg gcctcgaata cgatgtagag
     5041 ggtcaggacc ttggtgatgg aggcgggagg gatgagggca tcggcattct gttcgtagag
     5101 cactcttccg ttggcggcat ccaccagaat cgcgccccgg caattgatcc ggcccgtgct
     5161 tccccgggcc gtgagggggg tccatgaaat ccccgccaga aacagtacgc acaagatcca
     5221 tattcctcgc ttccccatag gagccgctcc ctctacaacc gccacccgtc tttccccata
     5281 aaccgtttcc tctcttggcc ggactactgt cgtgccgacc ttcaggggaa ggaacctgcg
     5341 ggatggagtt cccttcttgt ctccgtgtct gtcccctttc tcgacggtgg tcgcgtttca
     5401 cgttcggccc tacaagtgac cgtgaaagaa atcatggcaa gcctgagacg gcctgtcaac
     5461 ggaatattgg ggcggcccaa cattacttta tgattttctc ccgatcctat tcacggaatt
     5521 cctcctcgca caacccggcc gtggagaagc cgccgatgcc gtcgacctcc tcgtaagcat
     5581 cgcaatcgga cgtgaagtta ttccaaatca agcaatcatt ttcatttgta gcaccgaagg
     5641 ttttgttcta aatgtgaaat gtttggtata tagaaaattc cggatgaaga tctcgaacga
     5701 atcgtcttcc gctcatcgag acaaacgggc atcggaccgg gaaaggaaga ggaagaggcg
     5761 atgatggggc gcatgttgga ttttctttcc aaggacatgg cgatggattt ggggactgcc
     5821 aataccttga tctatgtgcg aggaaaagga gtcgttctgg atgaaccgtc cgtggtggcc
     5881 atggaggtgg gcacctcaaa agtcctggcc gtgggcaaag acgccaagaa ccttacgggg
     5941 cgtcacggtc ggaatgtcca gaccctgcga cccatgaagg acggcgtcat ccacgacttt
     6001 gaagccgcga cctacatgat tcgagccttt ctcggcaagg tattcaaacg cctcccccta
     6061 tcccgcccga aactggtggt ggccgttccc acgggcatta cggccgtgga gaagcgggcc
     6121 gtcatggagg cttcggagat ggccggggca ggcaaggtct tcctaatgga agaacccatg
     6181 gctgcggcca tcggcaccgg cttgcccgtg gatcaatccg tgggtcacat ggtggtggac
     6241 attggggggg gaaccaccga ggtcgccatc atcagcaagt tcaccgtggc ctgcagcgag
     6301 tcccttcgaa tcgcagggga cgaagccaac gaggccatct gcaggttcgt taaggacgaa
     6361 catcggatga gcatcagcga caccatggcg gaggtcatca aaatcaagat cgggtccgcc
     6421 gttcctctga agaggcaact ggaggtcaag ctgcggggga aagacatttc cacggggatg
     6481 cccaaggtcg tcagcatcac cgacgccgac attcgagacg ccatcaagat gcccaccatg
     6541 gccattgtgg aagccgtccg ccgggtcttg gagaaggcgt cccccgattt cgcctcggac
     6601 atcggggaaa acgggatctg gctcgcgggg ggaggagcgc ttctcaaagg gctgaggctt
     6661 cttctccacc aggcgacggg cctcgatgtc aagaagtccg aagatccgct ccgcgcggtc
     6721 attcgcggga cgggggccgt gaccgagcgg ttcgattttt accgggatgt gtttctcagt
     6781 tgaggcggga cgtgcctcgg cctgtgcccg gcccctcctc gtcaaacatt gacagggttt
     6841 gtcatttccg atagcatttt ctgcacaaga tgcaccgcgc ggaccgcgaa gaatccggga
     6901 ctcggaatgt gcgccatatc ccatctcacc tgtttgccgg ttcactggga aggatttcaa
     6961 gccgctgagg aactgggatg atcatagggc ttgacgtggg tggcacccat acagacgtgg
     7021 ttcttttggg gaaggaccgc attcttcagc aagccaaggt cccgacccgg ccgtccagtc
     7081 ttctcgagag cgtttggagc ggcatcgagg aggtgacccg gggcatagcg cccgaagcca
     7141 tcgagcgggt ggtgttgagc accacgttga ccaccaacgc cattgccgag ggcaagctcg
     7201 ccgaaaccgg gatgatcgta tcgagcggtc cgggcatcga tcccgaaggg ttccggacct
     7261 gccgggaatt ccattgcgtc aggggttcca tcgaccaccg ggggcgcgag attcagccca
     7321 tcgaccgtgc ccaggtcgaa tccgccgcgg acgggatgcg gagccggggg gtccgttacg
     7381 tgggggtggt gggcaagttt tccatccgaa atcccaagca cgagaatgaa atcgttcgca
     7441 ttctggggga ccgtttcgag cacgtggttt tgggacatcg tctttcggga aacctcaact
     7501 ttccgcgcag ggtctgcacc gcctatctca acgccgcagt gtaccccgtt tacaagaatt
     7561 ttttcgaagc cgtcagcgcg tctctgaaga agaagggtct gcgcatcccc atttacatcc
     7621 tcaaggcgga tggcggcacc gtgaccctgg aagcttccct gagctcaccc gggcaaacca
     7681 tcctctcggg gcctgccgcc agcgtgatgg gttccgtccc ttacgcttct cgggaggagg
     7741 agaccctggt cctggacatc ggggggacca ccaccgatat ggcggtgttg gtccggggag
     7801 tccctctcct cgatccggtg ggcatcgaac tggggggcta caagaccctc atccggtccc
     7861 tcaagactca ctccgtggcc atcggagggg acagtgcggt ccgactggag gacggtcgga
     7921 tccaggtggg acccgatcgg cagggtcccg ccatggccta tggcgggacg gtccccaccc
     7981 ctacggacgc tctggcggtc atgggaaaag ccgccggggg agattcggaa agggcccgcc
     8041 aggggctcct tcccttggcg cagcaactgg gagtgccttt ggaggaagcg gcggcacgga
     8101 tttttgatca tacctgcaag cagatcctcg aggcggccgc ccaaatggtc gccaacatca
     8161 accgaaagcc ggtctacacg gtccaggaac tcctggaagg ccaccaggtc cgacccacgg
     8221 aaatcctcgt tctgggagga cccgctccgt tgtttgcctt ccgttttgcc gccatgaccg
     8281 atttccgggt gcgggtcgtg ccccggtggc aggttgccaa tgccgttggg gccgctttgg
     8341 ccaggagcac ctgcgaggtg acccttttcg tggacacgga acgggggttc gccgtcgctc
     8401 ccgaagaatc ctttcacctg tccgtacgca gggatttcga caagcagagc gccgttcgcc
     8461 ttgctttgga actgctgcgc aagaagtgca cccaggaagg atcggcggat gacgggacgg
     8521 aaatggaact cgtggaggaa ctccagttca acatggttcg gggtttctac accaccggtc
     8581 gaaacatccg ggtcaaggcc caggtgaaac cgggactcat cccggaacat gcgcagatcg
     8641 ccgccggtct tttccatgcc ggacccaccg gcgggcagga tcatgaggaa gactcccatg
     8701 gttgagaccc cctccaaagt gggcctggtt ttcttccccg cctacgactg ggccatcagt
     8761 cccacgcacc ccgaacggga agagcgcctc ctgtacaccc aggaccaggt gtttgaagag
     8821 gggatcctgg acctcgaggg catcgtcgag ctcaaaccgg acctagtgga cgtgcgggac
     8881 attcgaaggg tgcatttctg cgtgccggag gaagggaggg tggtgacgga atcccacctc
     8941 atcagtgccg gaggcgcgaa gaccgtcgga atcgaggtcc tggagaagag ggtccgtcga
     9001 gggtttgcct tggtgcgtcc gccgggacat cacgccatga gggtggttca cggggctcgg
     9061 gggttctgca acatcaacat cgaagccatc atgatcgaat acctgcggga agcgtacggc
     9121 gtcgaccggg tggccgtggt ggacaccgac tgtcaccatg gggacggaac ccaggacatc
     9181 tactggcacg accgggacac cctctttatc tccatccacc aggacggccg caccctctac
     9241 cccggctcgg gatttcacac ggagatgggg ggtcccaatg ctttggggac cacggtgaac
     9301 attcctttgc ctccccggac ttcttccgag gggttcctgt ttgtcctgga cgaggtgatt
     9361 ctccccctgt tggaggagtt caagcccgag atcgtcgtca attccgccgg gcaggacaac
     9421 cattacacgg accccattac caacatgtgc ttctcggccc agggctatgc cgagctcacc
     9481 cgccgcctga agcccgacat cgcggttctg gagggcggat attccattga aggggccctg
     9541 ccctacgtga acgtggggat catcatggcc ctggcgggac tggacaccag ccatgtcaaa
     9601 gagcccgact acgacccgaa caaaattcgc cagtcggcgg ccacgactcg ttatatcgag
     9661 agcctggtga aggagatccg cgagactcga aggaggatgg cgggaggggg gaaagcccgg
     9721 tccggcgact cgacggccca acggcgccgc aacattttct acgacacgga tcaaatcctc
     9781 gaggcacagg aggagaccct ccgtctatgc gaccgatgca acggagtcct ggggatcgat
     9841 tcccgcacgg accggggaaa tcacatctat gcggtcatga ttccccggaa ggcctgcaag
     9901 gagtgttgtg aactggggta tgagtggttt gattccgccg accatcgaaa gtataatatc
     9961 acgtttttgc aggatcgaac ccaagaccag taccttgtcc gaaaggccta aacctcttga
    10021 agacggacgc gatcctcatc aagaatttta cccttcagca actggaagac tgggtcgaga
    10081 gcgtggggga acgccgcttt cgagcccgcc agttgtaccg gcacctttat gtccggcgaa
    10141 tccgatcctg ggatgaatgc agcgatctca gcaagagctt tcaggctcag ctcgagttcg
    10201 gcaccgtgct ggacgccctg tccctcgaag agaagcgcac ctcttcggac gggaccgtca
    10261 agtacctttt cgggctttca gacggccgca ggatcgagtc cgtcctgatt ccggacccgc
    10321 cccgctacac cctgtgcctg tccagccagg tgggctgtgc cctgtcgtgc cgcttttgcc
    10381 tcacgggaac cgtgggcttc caacgaaacc tgtctgccgc agagatcgtg gaccaggtgt
    10441 gccgggttca gcaggaggcg gggaaccgct ttcggatctc caacctggta ttcatgggca
    10501 tgggagagcc cctggccaat tacgaggccg tttcgaacgc cgtgcacatc ctgtgcgatc
    10561 ccaacgggat ggccttttct catcgcagga tcaccgtgtc caccgccggg ttggtccccc
    10621 aaatggagcg cctgggaagg gacagtcccg tcaacctggc cgtcagcctc catgcccccg
    10681 aagacgggct gcgctcggag ctcatgcccg tcaacaggac ctattccttg ggggacctcc
    10741 tgaacgcctg ccgacggtac cccctggctc cccggaaacg catcaccttc gagtaccttt
    10801 tgctcgacgg ggtgaacgac cagccccggc acgccaaggc cttggtcaag cttctccacg
    10861 gcgtcaaggc caaggtcaac ctgattccct tcaattcgta cccgggatcg gaatttcgca
    10921 ggccttcgga agagcggatc ctggcattcc aggagatcct cacgggggca caattgaccg
    10981 ccctcgttcg gcagagtagg ggcttcgaaa tcggggcggc gtgcggccaa ttggcggccc
    11041 tgggcaagga tgacccctcc tctcgagggg aaaccgaagc cgggcatggc tttttctcgc
    11101 taccgggata ggattcccgg aaccttccgc cagggtcccg ggagctcccg gaaggtcccg
    11161 ttggttggct tccaggtgga gtgattgaca ttgaaacccg actttagtag aaatcaagga
    11221 ttgctcccag ttatcgtcca agacgtcaag agcggtaggg tcctgatgct ggcatacatg
    11281 aacgagcttg cgtgggacaa gaccatcgaa accgggcgtg cccattactg gagccggtcc
    11341 cgacagaagc tttggctcaa gggggaagaa tccggccatt ttcaagtggt ccgtgaaacc
    11401 tacctggatt gtgatctgga caccatcctg ctcaaagtgg agcaaattgg aggagccgcc
    11461 tgccacacgg gcttccagag ctgtttctac cggcgtctgg agggggcgga ggtcatcgtg
    11521 tccgaagagc ccgttttcaa tcccaaggag gtctacagga agtgaatcag ctcgtgctcg
    11581 gcattcccaa gggaagccta caggaagcca ccattcgatt gttccgccag tcaggctggc
    11641 ggatttccct ctcggaacgc aattactttc cggaaatcaa cgaccctgag atcaactgcg
    11701 ccatctgtcg cgcccaggaa atgtcccggt acgtggaaaa cgggaccttc gacgtcggcc
    11761 tgaccggccg ggattggatc atggaaaacg actcggacgt ggaggtgatc gcggacctca
    11821 tctactccaa ggtcagccag cgacccgctc gatgggtcct ggccgtcccg ttcgaatccc
    11881 ccgtccaggg gctccgggac ctggaaggca agaagatcgc cacggaactg gtgaacttca
    11941 cccgacggta cttctcctcc gcgggggtga acgtccaggt ggaattctcc tggggagcca
    12001 ccgaagccaa ggtgatctcg ggtctctgcg acgccatcgt ggaggttacc gaaacgggca
    12061 ccaccatgag ggccaacggc cttcgcatcg tcaaggagct catgcagtcc aatacccaga
    12121 tcatcgccaa tcgaaacgcc atcgaagacc cgtggaagcg cgagaagatc gaacagattt
    12181 ccatgttgct ccagggcgcc ctgcgggccg aggaactggc ggggctcaag atgaacgtgc
    12241 ccgaggagaa gctgaaccag gtcatcgaga tcttgcccag cctcaacgct cccaccatcg
    12301 cccacctcta tcggagcgat tggctgtccg tggaaaccgt gattaccaag agcaaggttc
    12361 gggatttgat tccccaactc atcaagcggg gagcggaggg tatcatcgag tactccttga
    12421 ataaggtgat ctgatgagac ttagaagagg cttccgtgtg ctttgtgtgg cggcactcct
    12481 ggtttcagga ggctgcgcca cgttcaagca ggaacccagc tcgaagtaca ccgccgagca
    12541 actgaaggaa atgggggaga aattcctgac ggcgggcgct cttccccagg cactgaggct
    12601 cctgaccatg gccgaagcca aacgtcccaa ggacccggtg attcaatacg atctggcgac
    12661 cgcctacgac cagaggggac tctcttccga agccctggcc cattaccaaa aggccctggc
    12721 actcaagccg gacttccccg aagccctcaa tgccctgggg gcctattatg ccgctcgggg
    12781 tcaatacgac ctggccgaac cctgctttca gaaagccctg gcaaatccgt tctacgaaac
    12841 cccgcacctc gccttataca acctggggct ggtgtatgaa aagaaggggg acctcgagac
    12901 cgccctcaaa cagtaccagc aggcggtccg atttcagccg gcctatggcc ccgcctatta
    12961 ccgcatggga gagatcctgg aagtccttcg ccggggagac gaagcccggg aggcctacgg
    13021 aaagaccatc acctacaatc cggacatggt cgaagctcac tttcgctacg gggtcatgag
    13081 ctacatggcc ggagagctgg aaaacgcgtt ctattccctg agcaaggtgg tccgcctggc
    13141 gcctcacagc agcatggcca ccgattccaa gaagtacctg gagcagttgc agaccctgac
    13201 cgtcgaccgg cgaaagcccc ccaccgtgcc cacttcggaa aagctcgctc acctcgaggt
    13261 catggctgaa caagaggtca tgcagcaaca gacaagagcg ttgccgcctc cccccatttc
    13321 cgagtccacg ctttccgcga cgccgggaac cccggccccg acaacgtcga ctcccgccgt
    13381 taccccggcc ccggttgcgg cggccgaggc tacgtccccg tcggggacgg gaaccccggg
    13441 gacaggcgag gaagccaagc accccaagaa atctgccgcc tccccggtct cgactccgcc
    13501 ggccgcatcc ccggcagccc cgtccccggc tcccgccccg gaatctcccc ccgctgcagt
    13561 caagtccccc aaaccctcgg gcaagatctc gccctcggac atggaatgga cgtacatcgt
    13621 gcaggtgggg tcctttctcg acaaggagaa cgcggaaatc ctcctgcgcc gtttgaagga
    13681 caagggatac gacgcggttc tcaagaactt caaccaccag ttcctgggcc tcgtctacgt
    13741 ggttcagctc aaaccggtgg acgaggtggc caaggcgagc actctcatgg cccaagtgga
    13801 aaacgaggaa cagcaggtca agcccatcat catcaaggtt ccctcgaata tttagggatt
    13861 ttgagcgacc tccatccgtg ggggctccct tcttcatgca gactctcatc cagcgaggat
    13921 actcaagctc atgttcatgc gcaaagattc cggcgagaaa ggcgagaaga cattgatttg
    13981 caagcgggtt cggggcatca agcccttcat cgtgatggat gtattggagc gcgcgcagga
    14041 attggagcgc gccggagaaa acgtcattca cctggaaatc ggggagccgg acttcgaaac
    14101 cccggaggcg gtcaagaacg ccgccgtgga agctctccgg cggggagaga cccgctatac
    14161 ccacagcctg ggcacccgga agctgcggga agccatctgt gaatattacg agcgtcagta
    14221 cgggataacg aagctcgatc ccgatcaggt ccttatcacc tccgggagtt ccccggcttt
    14281 cctggtgggc atgggcactc tcttgggtcc cggggacgag gtgattctca cggatcctca
    14341 ctatgcctgc taccccaact tcatccagtt cctggacggg acccccaggt ccatcccggt
    14401 gctggaagag gacggtttcc aatacaggac cgaagccatc cgcaaggccc tgactcccaa
    14461 gaccaaggcc attctcatca actctcccgc caaccccacg gggaatcttc tggatgccga
    14521 ccgtatggcc gagatcgccc agctggggcc catggttttc agcgacgaaa tttatcacgg
    14581 gctggtctac gagggccggg cccattccgt cctggaattt accgacaatt gcatcgtgtt
    14641 caacgggttc tccaagcagt ttgccatgac ggggtggcgg ctgggctacc tgattgtgcc
    14701 ccgccccttg gtgcggacca tgcaaaaggt cctgcagaac tttttcattt cggccaattc
    14761 cgtggcccag gccgccgggt acaccgctct gacggacccg tccgtggagg ccgatgtcca
    14821 gcgaatgcgg gaaacctaca accgtcggcg caagttcatc ctccggcgtt tgcgggagat
    14881 cgggttcggg atctccgtcg aaccgacggg ggccttttat gcgttcgcca acgccaagcg
    14941 gttcaccacg gactcgtact cgttcgcctt cgagatcctg gagaaagcca aagtgggcat
    15001 cacccccggg gtggacttcg ggccgggcgg ggaaggctac gttcgattta gttacgccaa
    15061 ttccatggcc aacatcaccg aggggctccg gcgcatcgaa gagtatctcc ggaaccgttc
    15121 ttaagaacac ttcatggaaa acgccctgcg gatcctatcc gccgagttcg caacatccgc
    15181 cctaaggatc gaccagtgcc ctcccccgga tctgcccgaa gtggcctttg cgggcagatc
    15241 caacgtcggg aaatcctccc tcatcaattg cctgctcctg aggaaaaagc tggtgcgcac
    15301 cagtcgaacc ccgggtcaga ctcggatgct caactttttc cgaatcaacg gggccttctt
    15361 ttttgtggac ctgcccgggt acggtttcgc caaggtttcc caggaaatcc gggcccggtg
    15421 gggccccatg atggagcaat acctcacggg ccggagctcc ctgcggggag tggtggtcat
    15481 catggacgcc cggcaccccg caactcccga cgatcttgcc ctttggaagt ggctccggga
    15541 tcgaaatatc ccgggggcgg cggtcctcac caagatcgac aagctgagca gaaacaagag
    15601 gccctcccat gtgcagcagg cgtccctggc cttgggggtt ccccccgatc ggttggtcct
    15661 gttttccgcc gtcacccagg aaggccgaga agcggttctg aacctgttgg aatcgtggat
    15721 cgccccatcg caggggtagt tgacaatctt cgtggtttct ggcactgagg atgcttcccc
    15781 aaaacaggat catccaatgc cgtcaaaaga tcgttggctc cggatcattg acagattcat
    15841 gaaggcggcc ggcgggatcc cccgggaatg gacacggacc ccggcgcgcg cggcaggtct
    15901 cctgtggtac tggaccgatc gccgccatcg aaccgtcgcc cacaagaacc tctccatcgc
    15961 cttcggcggc accctggatg agcgggcacg gcgtcgactg tgccgtgaga gcttccagca
    16021 cctcgttcag gtcttttttg aactgcccta tgtgtattgc ctgaatgcct ccgacgcggg
    16081 tcgattcatc acctttcagg atatggaaca cctctatcgg gccatggaaa agaagcgagg
    16141 ggtcctggtg ctggcctctc acttcgggaa ctgggaattg atgtccatcg gcttttccct
    16201 ccttcagttt cccatccacg tgatcgccag gcccatcgaa aacccccttg tcgatgcctt
    16261 tgtcactcgg ctccgatcca tcgggggcac ggaggtcatc tccaagaagg gttcctttcg
    16321 ggaggttctt cgggtgctca atcgaggccg cgtggtcgcc attctcctgg accagaacgc
    16381 ggcggcccac gaaggagtgt ttgtcccgtt tttcggcaag accgcctgca cccacaaggc
    16441 aatggcaatc ctggccctca gaacgggggc gccggtggtt ccggtgtata attttcgcga
    16501 gcccggagga cactatcgca tggttttcct cccggagatt cccctgagag tcaccggaga
    16561 catggagagg gacatccggg agaacacttc gctgtataac tcaaccatgg agggttatat
    16621 acgcctgtac cccgaacagt ggttttgggt ccaccgtcgt tggaagaccc aacccggggt
    16681 ttcccgtgca cggcagaagg ccgaatacgg cgtgtgacag atcccgggag gaagaccgac
    16741 ggccgacggg caacctccag gccggagcga ccaagagccg gggccgaagc aggtttgaag
    16801 tggacaagcc tttgaagatt ctcatcgtga aagtgagctc cctgggggat gtggtgcaca
    16861 ccctacccgt gctctactat ttgaagagcg ctcatcccgg ccttaccctg gactggatcg
    16921 tcgaggaacc tttctcccag atcctggtga accatcccct cctgcaccgg ctccatcttc
    16981 ttcacaccaa gaggtggcga aagcacccct tcgagtcttc cggtcttcgg gaggtccttc
    17041 gacttcatcg ggcggttcgc aaagaacgct acgacgtggt cctggatctc cagggcaaca
    17101 ctaagagcgg agccgtcacc ctcctcacgg gggccccctc gaggtttggc ttcccggctt
    17161 cccacgttcg ggaatggccc aacctcctgg caaccaatcg aaaggtccgt ccggtttccg
    17221 aacttcttca catcacggac cgcaacctgg ccgtagcgca ggccgccttt cccggcgggt
    17281 ctcccccacc cccctcggga taccttttcg cggaagaaag ccgacaccgg gaagtggacg
    17341 aatactttga acggcaccga ctgggggatc gacccaccgc ggtgttcctc tacggagcca
    17401 gctggaagac caagctgtgg gaccgggatc actggaaacg gctcgtcgct tccacggtga
    17461 aggacctggg gatggatgtc cttctcacct gggcgagcga ggaagaacgg gcggcgtgca
    17521 tcgatatccg gggctcatgg gacgggcggg tgcttctttg gcccagaagc catctcaaga
    17581 cggtcctggc tttgttggaa aaggcccacg tggtggtcgg cggagatacc gggctcatcc
    17641 acatggctgc cgccgcgggg actcccacgg tctccctcta ctttgtgacc agcggtcgtc
    17701 gaaacggacc ccggggcccc ctgcatcgtt gcctacaatc gagcatgccc tgttcgccgt
    17761 gcctgaagaa aggatgcggg caccgggttg catgcggccg gagtatcccg gtttccgcgg
    17821 tattgtccgc catcggggag gtccttgaca ttgggagctc cggcggatca aggggatcaa
    17881 ccccgggaac ggggacctaa gagcgccatg atttttccca tggtacagga aatccgattc
    17941 gagacggacg aagccttgtt ccggcaaagc gggtttgaaa cctacgccca tttcatggct
    18001 cccgacacgg ggacccacgt ggccagaaag agaggtcggg tggttcgccg gttggacttg
    18061 gaaggaaggt ccttctttct caagaggaac cggttccatt ggatcgagtt cttcaagggc
    18121 ctcctgcaca tgagatttct cccccgcaac ggcctgcggg agtggcgaaa cattgacctg
    18181 gtcacccggg ccgggattcc cacggtccgg cgggtcgcct ggggggaaag gcgatgtctt
    18241 tcccgggaaa tctcttcctt tacgatcacc gagaagcttt acggtgcaag atccttgaag
    18301 gagattctcc aggagcgggc tgcaggcaaa gcggatgcag gttccttccg ggaaaagagg
    18361 gacctgatcc aaagggtggc ccggttggct tcccggctcc accgtcacgg cctgtatcac
    18421 caggatttct acctgggcca catctacaaa ggtccggacg aggtccttta tctcattgat
    18481 ctccaacggg tcctttgctc ccccttggct tcccgccgtt accggatcaa ggacctggcc
    18541 cagatccact attcggccat ggagattccc gggatctccg cttcggaccg cctccggttt
    18601 ctcctggcct acaccgggtc ccagcgggtg ggacgcgagg aacgggagat gatccaaaaa
    18661 atcctggcca aaaccgggcg aatcgcccgg cacaccgtga agcttctcga acgaagaagg
    18721 cgccgcaagg aaatcccatg accctgctct ccgtcctcat cgtgaactgg aacacccggg
    18781 acgatctccg ggaatgtctt cgatccatcg gcgaaaacct gacatccctc cgcccccaag
    18841 tgatcgtcgt ggacaacgga tcccgggacg gcagcgcggc catggtctct tgcgagtttc
    18901 ccgcggtgac cctcctttcg agcccaacca acctgggatt cgctcgagcc aacaaccggg
    18961 ctctccaaca ggcctcggga gacttcctcc tgttgttgaa cccggatacc cgggtgggga
    19021 aaggagctat cgaggggctg atcgagacca tggccgccga tcccgccgtc gggatcgcgg
    19081 gcctccagct cctcaattcg gacggatcca agcagaattc catctccagc gatccctcgc
    19141 ttctcacgga actcaccaac aagagtcttt tgcggatgct ctttccccga aagtacccgg
    19201 gaaaggaact ggagttgact cggcccatcg acgtggatgc cgtcatcggc gcgtgcatgc
    19261 tggtacgcag aaaagccttc gaagaggtcg ggcccctgga cgaggactac ttcctcttcc
    19321 tggaagagac cgactggtgt cgacggatgc gaaggaaggg ttggaaggtg gtccacgatc
    19381 cgcggtttac cgtcctgcac ctccagggta agagtgccgg tcaggtcccg acggcggcgc
    19441 ggacggaata ttggaaatcc cgttatcgtt tcttccaaaa gcattacgga ccgggggttc
    19501 acgcgacgtt aaaggccggt ctcatcctga agcttgccgg gtcctgtctt gtcaacacgg
    19561 tgctgggact ggccacgggt tttcttcatt ccaagacccg gcagaggctc tttctcaacc
    19621 tccgactcct ggaatggcat ttgcggggat gccctcccga ttggggactc cggccgccgc
    19681 aatcctccca tagtctttag ccggattcgc ggacaggggc ggcaggtcca ggattctgtc
    19741 ggaaacgatc gggggaatcg gccggcatgg gcctcagcac cacgatgccg tcgaaatgct
    19801 cggggatgct ctccgcgacc acctccccgg ggaggaaccc ggccttcttc atgcgacggt
    19861 accggtcccg atccaggatg aagtacaggg gttttcccgc ctcctcggcg aggacccggg
    19921 ccaggctccg gtccggatag acatggggaa accaaaggac ctctcggtcc aagtagaaca
    19981 ccaggtcgtg aggttccgtc tcgaccatca ggagctttcc ctcctttccc acgatctcct
    20041 ggatctgggc cgccagggtg cgctgggtgc ggaacggttc gaaaaacggg gcgatcttga
    20101 cttcgtgtac ggtgtggaaa gccgccgcca ggaccacgag aagggaaaag gcccatttca
    20161 gcgaacggcg tttcaggaag attcccgcaa cccccgccgt catgaggacc gagaacaaca
    20221 ggatggacac acgattttcc ggtgcttgga aaaacctcag gtaggtcaac agcccgttgg
    20281 cgtcgtcgct gttgaaccgg gattcaaacg ccgggtgttc aaccagggtt tgtacggggg
    20341 agctcaagga atcgccaaag gcggtgagga gcgccaagag ccacaagacc gccgtcgccg
    20401 tcgccaacag gatgccggtt gccgtcaggg cggtgcgaat ccccttgccg tccggatcct
    20461 cctcgtagaa attggccagg caccacgcca acagcaggga cagcaggggc agaacgggaa
    20521 ggaggtaatc ggcccgtttg aagctggaga gactcagaaa caggaaggaa accccgcccc
    20581 acaccaccag gaaccggcgc agatcgtaag aggggcggtc tcttttgagg acttttcggg
    20641 gcaaagccgt gagcagcaag gaccagggca gcatggaagt gaggagatga gggatgtaga
    20701 accagaaggg gcggtgcttg agggcttcat atcccgcaac cccctgggga aaaaaccgga
    20761 cgaagttatg gtagatgaaa aaatcccggg tgaactgtcc ctgggtctca tgatggaccc
    20821 aaaagaacca gggcgaggcc accaggagaa gcacggcaac ccccaggagg tgaggcgaaa
    20881 agggccgcac cgcaatccgc ttccggaaga ggccccccag gaccagccct cccaaaggaa
    20941 ccagaacggg ccatccctgc cgcccgagac agagccccgc gagcaccagg gcccccgcga
    21001 tccaggcgag gcaaagctcc ccgatcactt cccacctttt ttccaggaga aggatgggtc
    21061 cgatcccgca gaagaccagg acgatcccca ccggcccctt ggccagcaca cccaggccca
    21121 tgagaccgta ggcagcggcg cacagaatcc aggaacgtcg ggagcgttct ccccggcagg
    21181 caaagtaaag ggcaaagccg gatgcgatca ccaggagagc cagaaccatg tcgatacgcc
    21241 ccacccgggc aagccagcga taatggatgg aagtcatgag aatgagggag gccatgacgc
    21301 ccaccgggag cgaaaacatt cgggcaccca tccagaagac cagggccacg agcaggacgg
    21361 cgctcacggt gctgggaaga cgaatggtga ggttgttcac ttccccggtc atccgggaaa
    21421 acaggctgac gccccagtag tagagcggcg gtttttcgtt gttgtacccg cgaccggtct
    21481 cgaagcgttc caccccgtag tccccgcttc tcaccatggc ccgggcgatt tgccctgcaa
    21541 tggcctcgtg ggaagaccag atcccccgat cctccatttt gaacccgaac aggaaaagag
    21601 ccaggagcat cacccctgcg aaggaaagcc accgaatctt ccggaaaggc ggagggccct
    21661 cgaaccccac gaaggaggga gcgtggaaga aggagccggg accctcaggg gagccttgac
    21721 cggatgggac ctgttttgtg cggttcatac cgtcgcgccg taagacttga aagaagaccg
    21781 gggagccgat gcagcctttc ccgatagcac gaatcccggg aaaaccaaag aggaaaagga
    21841 ccatttggga gtttacttca ccgggggtct atggcatacc aaagggcgcc atggacaatg
    21901 ggaggcaggg gatgaaaccc gcagacaccc gaggcactcc ctccttcaac cttccaagac
    21961 ctggttaaga tcgatccatg agggttcttt ttctggttcc tcgcctggac aaggcaagca
    22021 cacgctaccg tgtccttcaa tatctgccgt atctgcagga acagggggtt tcctgccgcg
    22081 taaggacctt gtccaaggac cggggatggg atcggtccct gtttcgggag gcccggtctt
    22141 cggatgtcgt gtttttgcaa aagcgcattt tgtccccttt cctgctcctg gccttgagaa
    22201 aggcgtcctc gagattggtg tatgacctgg acgacgccgt catgttccgg gatgaagccg
    22261 ccaccgaga
//
LOCUS       DAOOVC010000056        22056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1607_length_22056_cov_6.389346, whole genome shotgun sequence.
ACCESSION   DAOOVC010000056 DAOOVC010000000
VERSION     DAOOVC010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 22056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22056
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1607_length_22056_cov_6.389346"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="PLM79_09050"
     CDS             complement(<1..2004)
                     /locus_tag="PLM79_09050"
                     /inference="COORDINATES: protein motif:HMM:NF040127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HOV86496.1"
                     /translation="MKGIERKISANIIPLLVFLLLPAILLPFASTCPAATFGWMPGRV
                     GDFHGPNWYEDGVDPPVENVLIPLRSAVGYIEKGGTVLFKGGSFDDGYITLYRGATFN
                     MTGGSLGSSADNGVMLRMGDKLRRGGDFLLRGGTCNFENLDIYGSTFRQTGGTIKVSH
                     GELLLGQQGKLEITGGMFRCNQMVNWGDFRAGDSAKVLIERWIGSNAGHAVFSGNARV
                     ALGMANIRSLAITDSSVKVKANSLAVGDWEVRTNYQAPRGTTILISASASGQGALIVN
                     RVKGGGIENTHFITSSPMEMGKSVSVYTSYSQDRGCTSVGFLDNGAIGTITVKKGLTQ
                     ACSPVLYVRELHIGRNARLSYYGKLYYGKLVVEPGGELVPAGPGAKSCRADPPVTRYL
                     AVTIDPPEAGSVRGAGGKIHCPRNCSGQFPDGFTTYLTAETKSGYVFDHWEGPLTTRT
                     NPLPVALDFGDESYRAVYKSLFPPDDTGHGRLGDPVNTATGEHYFNLPVFDLGGPLPL
                     SFHLTYGSSLHDRPNISETFGGTLGHNWMHNFQATRIVDSKTHTRIVYDRGKTLSFHA
                     GPSAGEWLLDKGEKAPYQLRSVGKRFVLMDPEKELVYAFDAAGRLDFIEDRNGSRLNL
                     TYDPAGRLLRVGDGLGRRLSFSYDGSRLTDVCDGYGRCASFGYS"
     gene            complement(2059..2361)
                     /locus_tag="PLM79_09055"
     CDS             complement(2059..2361)
                     /locus_tag="PLM79_09055"
                     /inference="COORDINATES: protein motif:HMM:NF017411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HOV86497.1"
                     /translation="MKAVYFVVIACVFYALILPCDSLSRAASMDCTYDEAGRLIRVEY
                     GNGAVIEYTYDRRGNVLKNKVSTLHTSMSMADVPGEAASAPDGDEGDVQIRLDGMP"
     gene            3044..3712
                     /locus_tag="PLM79_09060"
     CDS             3044..3712
                     /locus_tag="PLM79_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOV86498.1"
                     /translation="MKFFCPGCRVTFSVPDEKIPAGKELKILCPKCRMPVEKKEEPQQ
                     EGGPEKDGAEPFPSYASAELDAQEEDSSFLEVVEDGVKTALLCLSDPSRTEQVEQMLR
                     QSEFYVSTATSVPMALGKLRSNHYDLLVLDDKQQGSGPVGNPLLYHIQLLPMHVRRQF
                     FLCLLSESLPTMDRLTAFRQGVDLILNVKDLDKAKILLVRSMRDHEGFYKVFNDELGR
                     RGQR"
     gene            3727..4023
                     /locus_tag="PLM79_09065"
     CDS             3727..4023
                     /locus_tag="PLM79_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86499.1"
                     /translation="MVFKRLFGRKAAGHFRLRIENCISTILDVNERLGEGKIRPEILD
                     QFSRLKESIQYLSDDNVDEENIHRIEEATNQLLAEVRSSCGSKLERGFSGDSVH"
     gene            4137..4673
                     /locus_tag="PLM79_09070"
     CDS             4137..4673
                     /locus_tag="PLM79_09070"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOV86500.1"
                     /translation="MAWREEKVDVFVFSRKGDEGETSLLTGARVSKASLRPEAYGTLD
                     EASSALGLAKTLTENSSIRQMIETVQEDLVLLGGELAWEGDEGKYRIGTDRTRRLEEW
                     IETLQREVPIPRQFIYPGCNPSSAAIDLARSVIRRAERRVVAMKEQGLIRDPDIHAYL
                     NRLADFLFTLARYAEKRG"
     gene            5069..5425
                     /gene="rpoZ"
                     /locus_tag="PLM79_09075"
     CDS             5069..5425
                     /gene="rpoZ"
                     /locus_tag="PLM79_09075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOV86501.1"
                     /translation="MARVTIEDCLEKVESRFSLVHLAVRRVLQMRSGAPLMVNAPKNK
                     EIVLALREIAAGKVTPENIRELEESKVLAEPAVEEKEEVTRREVKEILEAATQYDASL
                     EYEEAAPPLEPDEDEL"
     gene            5563..5946
                     /locus_tag="PLM79_09080"
     CDS             5563..5946
                     /locus_tag="PLM79_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86502.1"
                     /translation="MSRQEILSCLEKRDLLNQPAVAVDKLLHWGRVFEEAEMLSDALD
                     FFEKADSRDDLLRLFARAREEGDLFLFRRLAKALNHEPTSDEWLSLARRAEEIGKTAF
                     ALEAYRSGGHDPSKGESPEGSPVPT"
     gene            6125..7279
                     /gene="dnaJ"
                     /locus_tag="PLM79_09085"
     CDS             6125..7279
                     /gene="dnaJ"
                     /locus_tag="PLM79_09085"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOV86503.1"
                     /translation="MNKRDYYETLGVSRNAGEEEIKKAYRKLALKYHPDRNAGDKEAE
                     ERFKEAAEAYEVLRDPQKRRIYDTYGHEGLQNSGFSGFRGFDEIFSSFGDIFQDFFSF
                     GFSGTRRTKSAARPGNDLVYDMTLTFEEAVFGTEKDIEIETLVECSGCEGSGAAPGTQ
                     ESTCPMCHGSGQVVQSQGFFRISTTCGRCQGTGRVLTSPCNQCRGQGRVRQVKRVQVR
                     IPAGVDTGTQLRLRGEGESGYRRGQPGDLYVRLEVRPHEFFERDGDNLYGKVPISFVQ
                     AILGDQIEVPTLDGRKAIQVEPGTQPGHVSRFPGEGVPRLRGNGRGDLFIELEVTIPK
                     HVGERQAEILREFMDLEAAKEGRKVKKWPWSRRKESDSDPVLNSHRRARS"
     gene            7315..7524
                     /locus_tag="PLM79_09090"
     CDS             7315..7524
                     /locus_tag="PLM79_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86504.1"
                     /translation="MRRSSVGQYEDFVASALYCPRCKCAMPVRSRLLLVLPDGELHEY
                     LCQACASSLGTKTVRNKQQTRIYLP"
     gene            7822..8772
                     /locus_tag="PLM79_09095"
     CDS             7822..8772
                     /locus_tag="PLM79_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86505.1"
                     /translation="MIETEGLTKFYGPLAAIRDVTFHIERGEVVGFLGPNGAGKSTTI
                     RILTCYMPATAGSARIDGTDCFEQSLEVRRKVGYLPENVPLYLDLTVRRFLRFAAGVK
                     GINGKDQEGEISRVIGICGLEKVSHRILGHLSKGYRQRVGLAQALLNSPPVLILDEPT
                     IGLDPAQIVEIRKLIHELREEHTILLSSHVLPEVAQLCDRVLIINKGQIVATDTPGNL
                     TAQLQKSAQIVLQVDGDAAGLAPALESLEGVQRVSFRENQHGRFWIETDRSRELRPEI
                     ARLVVDRRLDLLELKLVDLSLEEIFMQLVTEEPSQEESET"
     gene            8769..9539
                     /locus_tag="PLM79_09100"
     CDS             8769..9539
                     /locus_tag="PLM79_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOV86506.1"
                     /translation="MKGFVPVYRKELYSLFASPIFYVVAFTFLVIAGYFFYSAVAYYN
                     LMSFQASQSPMAARQLNVSDMVLRPFFLDLSIVLLLISPLLTMRLYAEERKTGTIELL
                     LTYPVRDLSAVLAKFAAVATSFLAILAGTLPGMFLLHMIADPSYPTIFCGYLGVFLMG
                     SAFLSLGVFTSSLTQNQIVAAVLSFGALLMFWVIGWTKNLVGPTAGSFIDYVSVIRHL
                     DNFAKGIVDTRDLLYYLMFIVLFLFLTLRQMESFRWRG"
     gene            9659..11122
                     /locus_tag="PLM79_09105"
     CDS             9659..11122
                     /locus_tag="PLM79_09105"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HOV86507.1"
                     /translation="MLKKKKTGRAWSYGSGTLISTLFFLGILVVLALIAEKNPRRWDL
                     TESKSYTLSEQTRKVLDSIDQPVSIKAFFATAAPEQPKVKDLLDTYVYYNPKITAEFI
                     DPDRQPEVAKQYEIRAYGTIILEGYGKKQPLPTATEEGLTNGILRLTRKTDKKLYYLV
                     GHGERSFENTAKEGYSTANYELQKASYALSPLNLMQQSQVPEDAVAVFVIGPQKNLFP
                     QEVESLKAYLERGGRALMFLDPYRDGGLRELIAGYGIELGDDMVVDKLSRVFGGSYLM
                     PVVMEYGGHKITKDFEVATFFSEARTVRPAKEPPANVHVEALASTSPNAWGETDLDLL
                     QKGQAGFDEGKDTAGPVSIVVIAEIASQQKKGEAQGKGPAQETPTPPDGSNSDDSDPS
                     GDAGRKGYLIVAGDADFADNTYFGLSGNGDFFLNMVNFMGEDENLITIKPRQKTGTAR
                     LMTPGQARTMFLTLLVFVPLTVVACGLLVYRVRRSQR"
     gene            11119..12492
                     /locus_tag="PLM79_09110"
     CDS             11119..12492
                     /locus_tag="PLM79_09110"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOV86508.1"
                     /translation="MKWRHSLLYLLVFCVVAGYFYYFEVVKNERKAAADKEAKRLFQV
                     ARESLDAVELHPKGQKSIVLKKEDRWKILEPVQAEADRTAVDDLVSTLSKLESEREVI
                     AAAQDLTPYGLADPSLKVRFHSQETWMELLVGEKNPTGSGYYVKKGDRPEVFLIAHSD
                     WGPMSRGVNDLRRKQLFTFEPGKIVGLRLAWQDGSIVDVKKQDKPPGWEIAGEPEVSV
                     KESKVRNAVEQVQWLRARDFPPEGPEDLKAHGLDSPFLRVDVDLGEGRTAFLLVGQKD
                     QDGKRVRAYSSELGAVAWIEASLLNELPKTPRDLEDRSVVGVKPDVVQKADLRLGDLH
                     RQAVRMDDKWGLAREGKGPEALKEPWQVRSLLWDLNQLEYVQKVEPVPPLPQNPFGRI
                     ELWSQTQLLAVMGWNQDETGPESLRTVWVEKDGNLKALRMKSEDLEKVRKAVQGILSP
                     SDKEASS"
     gene            12879..13283
                     /locus_tag="PLM79_09115"
     CDS             12879..13283
                     /locus_tag="PLM79_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86509.1"
                     /translation="MKRKATYRLMVTRREMLYVHDEFDHSLMLVEMEGEPIDYQVGVA
                     GEFVSRRSVTFHDRIRGNGPMKGYAITNFREGSVCTSYEGAREGDLTKGTWKAYKGIG
                     KLANLKGNGTFTVKPGQRDREYILEVEGDYDL"
     gene            complement(13525..15285)
                     /locus_tag="PLM79_09120"
     CDS             complement(13525..15285)
                     /locus_tag="PLM79_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOV86510.1"
                     /translation="MKMCRLAKIRIWSILCLVVLGTAPCWSAEPAGVPAKVAVLPFSM
                     HTPPELAYLQSGIRDMLSSRLAWQGKVQVLDRTAVTQALPSSKGDLPLEEALRIGRNL
                     KADYVLFGSITALGQSISIDAKMASVAGKSEPIHLYAQTKTLDEVIPKINQFAQDINA
                     KVFARPGEQRADAAGGEDREFAGLRNPELLVPETMLPGDKISYLNPNFIEVTPEGSLR
                     QGGLWRSQTITAGVVGMDLGDVDGDGKNEIVTVTMNKVTVYRREGQGLKTIGTFNGQK
                     VDRFLNVALVDLNRDGRAEIYVSNLRKYNTSRPVYGESAMGGGGGAVEDLASFGLSYD
                     QGKLQVVCDRVGYFLNGVDFPKRGKVLIGQEKGQITEGAFKPGIFEMQLRGKGLAPSS
                     PVGVSSRCNVFNFAKADINGDLMEETLFIDSANRLVVLSASGDQLWKGDRYFAATTNS
                     FEAKVMDRRFNQVDMYAIPSNIVVTDLNKDNIPEIVVTRSLDVLTRFLPEGLKHYERG
                     EIVSLSWDQMGMVENWKTREVNGMISNIRVGDLNNDGTTELVASLIMARDLLKLWESK
                     STIFSYDLNISPAKTAAKLP"
     gene            15707..17356
                     /locus_tag="PLM79_09125"
     CDS             15707..17356
                     /locus_tag="PLM79_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86511.1"
                     /translation="MKKYFMIFVALAVAVTLASPVLAAVEFKYGGQFRNRIDSSDNVY
                     DGSDKGSDNLNFIDQRLRLYFTFQASENLKLVWKMEVGDTTWGNDWPTAGTSTLGKGG
                     RVGSDAQNVETKNAYIDFNIPNTPVKIISGIQTLNLLNSWIVDDDFSAIVAKAKFEPF
                     AVTLGYVAAQNYDLSHYVDNIDSFFFAFDWVQKPFSASLIGYYQYGRDTVASIYPWFR
                     SPTNNVGNSSSSATFFPYYGSLAPGRTDMFRNGLLSVGDNNLFDLGFNFSYKIDWLSA
                     YVSFVKNLGSFDLNVANTYPFAPPSTNPSNYVKGTSMDYTGWMVDAGVTYYCAPWTAN
                     IGGFYTSGQESPSQQVVGNIFNGLPLDPYKLYFISLDSFKGNDLDRFVLPLAQFKYFS
                     EIIGGGVIGDTIDSGNGSWGNVQRGNFWQGFPNPFNIWTITAGGSYQLLPKTKLSASY
                     WYFGTSEDVPVALSRVLRVRPNGDIVASYDMDNQIGHEFDVYLDHEIVDGLQLTLVGA
                     YLVSGDAFAPINPTLNNNINTIFRKNASDAYELGARLQWNF"
     gene            17737..19389
                     /locus_tag="PLM79_09130"
     CDS             17737..19389
                     /locus_tag="PLM79_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86512.1"
                     /translation="MKRYFMILVALAVAVTLASPALAAVEFKYGGQFKNRIHSSNNTW
                     DGSDEGSDNLNFLDQRLRLYFTFQASENLKLVWKMEVGDTTWGNDWPTAGTSTLGKGG
                     RVGSDAQNVETKNAYIDFNIPNTPVKIISGIQTLNLLNSWIVDDDFSAIVAKAKFEPF
                     AVTLGYVAAQNYDLSHYVDNIDSFFFAFDWVQKPFSASLIGYYQYGRDTIASVWPWTR
                     SPLGNIGNSTTSAAFFPYYGSLVPSANDMFRNGLLSVSDNSLFDLGLNFSYKIDWLSA
                     YVTFVKNLGSFDVNIAKSYPLLPPSTNPTNYTKFRSMDYTGWMVDAGVTYYCAPWTAN
                     IGGFYTSGQESPSSKVVGNIFSGLPVDPYQLYFISIDSFKGNDLDRFVMPLSQTKYSS
                     EIIGGGILGDTQDRGNGPFTGDVQRGNFWEGYASPFNIWTITVGGSYQLLPKTKLSAS
                     YWYFNTSEDVPVALKKTFGIAPNGDIIARYEMDSQIGHEFDVYLDHEIVDGLQLTLVG
                     AYLVAGDAFCPINRTLSANPNQIFRKNADDAYELGARLQWNF"
     gene            19619..20632
                     /locus_tag="PLM79_09135"
     CDS             19619..20632
                     /locus_tag="PLM79_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOV86513.1"
                     /translation="MYLRRVRRGRGFRYILRESYRVGDAYRHRDLMDLGTNPEEYIVY
                     PGGNGFYFRFEVEESLRDQGIEYSSRDLDEAFLPFLPLHIRRVLGMFDHGTGGARTKK
                     LERTEDLRQLQEKLHPFDKRRLHYLRFGQMEIGGSQDQWFKFLHVLFRKSRDEIEHIL
                     EGMEQRLRPTELRNYLYAALELAAYFPHHRMRHYPGALDQEKVDECFLEELCRLNGDP
                     SYLLGADSPGGEPLHPYLVRYLILYFDSGDWAANPYWQYAQQFARGRFSRSRPFRAEL
                     APREACRVFGVSYETFQAMTRKELIRDYRRKAKESHPDRGGNHEVFVKLAEAFRCLLA
                     NKP"
     gene            20881..21111
                     /locus_tag="PLM79_09140"
     CDS             20881..21111
                     /locus_tag="PLM79_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86514.1"
                     /translation="MPEAELRKHSESNQIPYFVVDCIVEQPWGAHPSSVPFCHDYDAP
                     FLRTMDAASRNPEDLKAWLAEPQAAPARTGTR"
     gene            21151..21492
                     /locus_tag="PLM79_09145"
     CDS             21151..21492
                     /locus_tag="PLM79_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86515.1"
                     /translation="MGGYRFDDRGILYLDTLHPGVTVEKIRENCGFDLNVTRVKGETP
                     APTYRELFVLREFVDPELIFLPPKMDYLPVIRSFPFMKHFPPAAGPKAVWALHQIPPS
                     PVSMTSYSPNG"
     gene            complement(21708..>22056)
                     /locus_tag="PLM79_09150"
     CDS             complement(21708..>22056)
                     /locus_tag="PLM79_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86516.1"
                     /translation="GDDDVVREIRWFLESLEGIHSVLTSDHIMNLLEEVAGKLPEDKE
                     AMLAVIDRYLALPPEERLLFRLGRRGGALRSLDELQDPTTRERLQSAKRELESETGGD
                     LEQLITDLGDQYI"
BASE COUNT         4628 a         6194 c         6640 g         4594 t
ORIGIN      
        1 ggagtagccg aaggaggcgc atcgtccgta gccgtcacac acgtccgtca gcctgcttcc
       61 gtcatacgag aaggaaaggc gccgaccgag cccgtccccc actctcagga ggcggcctgc
      121 cggatcgtat gtgagattaa gccggctgcc gttgcgatct tcaatgaaat cgagcctgcc
      181 cgccgcatcg aacgcgtata cgagctcctt ttcagggtcc atcaacacaa atcgcttgcc
      241 cacgctcctc aattggtaag gggccttctc ccctttatcc aggagccatt ccccggccga
      301 tgggcccgcg tgaaaagaga gcgtcttgcc cctatcgtaa acgatccggg tatgggtttt
      361 cgagtccaca atcctcgtcg cctggaaatt gtgcatccag ttgtgtccaa gggtgccgcc
      421 aaaggtctcg gagatgttcg gcctgtcatg aaggctgctg ccgtaggtga ggtggaagga
      481 aagaggcagg ggaccgccga gatcgaaaac gggaaggttg aaatagtgct cgccggtagc
      541 cgtgttgacg gggtcgccga gcctgccgtg gccggtgtcg tccggcggaa acaaggattt
      601 gtaaacagcc cggtagcttt catcgccgaa atcgagggcc acgggaagcg ggttcgtcct
      661 ggtggtgagc ggcccttccc agtgatcgaa gacgtatccc gacttcgttt cggccgtgag
      721 atacgtggtg aacccgtcgg ggaactggcc gctgcaattc ctcgggcagt ggatcttgcc
      781 gccggccccc cgcacgcttc ccgcttcggg gggatcgatt gtcacggcga gataccgggt
      841 aaccggtgga tccgcccggc aagatttcgc gccgggaccg gcaggaacga gttccccgcc
      901 cggttccaca accagcttgc cgtaatagag cttcccgtag taggagagcc gggcattgcg
      961 gccgatgtga agctcgcgta cgtacaggac cggagagcaa gcctgggtta gtcccttctt
     1021 caccgtgatt gttccaatgg cgccattgtc gaggaaaccg acggacgtgc aaccccggtc
     1081 ttggctgtag ctcgtgtaaa cggatacgga cttgcccatc tccatgggag agctggtgat
     1141 aaagtgagtg ttttcgatgc cgcctccctt gacacggttc acgatcaggg ccccctgccc
     1201 ggaagcggag gcggagatca gaatggtcgt cccgcgggga gcctggtagt tggtgcgcac
     1261 ctcccaatcc cctacggcaa gactgttcgc tttgaccttc acggagctgt cggtgatcgc
     1321 aaggctccgg atgtttgcca tgcccagtgc aaccctggca ttgccggaaa acacggcgtg
     1381 acccgcgttc gacccgatcc aacgttcgat gagcaccttc gcgctgtcgc ctgcacggaa
     1441 atcaccccaa ttgaccatct ggttgcaccg gaacatgcct ccggtgatct ccagcttgcc
     1501 ttgctgcccc aagagcagct ctccatggct tacctttatg gtgccgccgg tttgccggaa
     1561 cgtcgagccg taaatgtcaa ggttctcgaa gttgcaggtc cctccccgca ggagaaaatc
     1621 tcctcctctc cggagtttgt cccccatcct cagcataacg ccgttgtccg cggaacttcc
     1681 gaggctgccg ccggtcatgt tgaatgtggc gccccgatag agggtaatgt agccgtcatc
     1741 gaatgaacct cccttgaaca agacggtccc tcccttctcg atgtacccca cggctgacct
     1801 gagagggatg agtacgttct ccaccggcgg atcgacgccg tcttcgtacc agttcggccc
     1861 gtggaagtcc ccgaccctcc ccggcatcca gccaaaggtt gcggccgggc acgtcgatgc
     1921 aaagggcagc agaatcgccg gcaggagcaa gaaaacgagc agggggataa tgtttgccga
     1981 aatctttctt tcgataccct tcatgatgtc ctcctgatgc cttggaatcc atccgtaagg
     2041 tttgccgccg aagatcgatc agggcatccc gtcgaggcga atctgaacat ccccttcgtc
     2101 cccgtcgggg gcggacgctg cttcccccgg gacgtcggcc atggacatgg aggtgtgaag
     2161 agtggagacc ttgtttttca acacgttccc cctgcggtcg taggtgtatt cgatgacggc
     2221 cccgttaccg tattccacac ggatgagccg tcctgcctca tcgtaagtgc agtccatgct
     2281 cgccgcgcgt gacagagagt cacatggaag gatgagcgcg taaaagacac atgcgatgac
     2341 cacaaagtag acagctttca taatgtctcc cctcctgagg ggggtgatag aagaggtgag
     2401 gcgagaggag gatgacggtt tcgtagaaaa ccctgaaaaa gcgtttcttc gaccgtaacc
     2461 gattgaattc attaggtgtc gttttgaaaa aaaacgactc tttgcgggac cgtcatagga
     2521 tgttgctcct cagaggttct ctggatagca ggtacggttg gttgaatcaa ggacatttgc
     2581 acctcgatcc acggttgcgt cacgcatccg gatactggcg tgaaattccg cgaaaagttg
     2641 tttttgccga acttccacat ccaactcagc catatagagt gatatgaatc aggagaacaa
     2701 acccgacaac ccaagcttga caacattcca ttgcctggag gtgcgccccg tcgcccgcga
     2761 ggagcggcgg ggctgggacc tgctcatgtc caggcatcat ggccccttgt ccgaattgaa
     2821 ctccgtccct gtgtttggtg gtgtcttttg gcctctacgc cgagtcttca cactctacca
     2881 cagcctcttg gccctttggt gtccaccacc gaacctccct cggaccttct gaaaacggga
     2941 aggtgaaatt ttgggattgt cagattatgg gcttcgcctg tatgttggca aaaccattca
     3001 aggacccata aaaagacccg gggagacctc gaaggagaca gccatgaaat tcttctgtcc
     3061 cggatgccgt gtgactttct ccgttcccga cgagaagatc ccggcgggaa aagagctcaa
     3121 gatcctttgt cccaagtgca ggatgcctgt ggagaagaag gaagagcccc aacaggaggg
     3181 cggaccggag aaagacgggg ccgagccctt tccttcgtat gcttctgcgg agttggatgc
     3241 ccaggaggaa gattcgtcct tcctggaagt ggtggaggac ggggtaaaga cggccctgct
     3301 ttgcctttcc gatccctcgc gaaccgaaca ggtcgagcag atgctgcggc agtcggaatt
     3361 ctatgtgagt acggcgactt ccgtgcccat ggccttgggc aagctccgca gcaatcacta
     3421 cgacctcctg gtcctggacg ataaacagca aggttcggga cctgtgggga acccgcttct
     3481 ttaccatatt caactcctcc ccatgcacgt aagacggcag tttttcctct gtctgctcag
     3541 cgaatcgctg ccgaccatgg accgcctgac ggcttttcgg cagggggtgg atctcattct
     3601 caacgtgaag gacttggata aggcaaaaat cctgctcgtt cgctctatga gggaccacga
     3661 ggggttctac aaggtcttca acgacgaact ggggagaaga ggacaacgct gacgggatga
     3721 ggggatatgg tttttaagcg tcttttcgga agaaaggcgg ccggacattt ccggcttcgc
     3781 atcgagaact gcatcagcac aatcttggat gtcaacgaac ggttggggga agggaagatc
     3841 agaccggaga tcctcgacca attctctcga ctcaaggaat ccattcagta cctgtcggac
     3901 gataacgtgg acgaagagaa tatccaccgg atcgaagagg cgaccaatca gttgctggcg
     3961 gaggtccgga gttcctgcgg aagcaagttg gagagaggct tttcggggga ttccgtccac
     4021 tgatcccgcg gctccgcccg atgacaggga aaaatcccgg aggcgggtgt tttcaaggcg
     4081 ctcccgttcc ggtacgagac gacgaaatct cgatctgtgt gtggaaatcc atggggatgg
     4141 cgtggaggga ggaaaaagtg gatgttttcg tgttttccag gaagggggat gagggagaga
     4201 cgagcctgct gacgggagcg agagtgtcca aagccagcct tcgaccggaa gcttacggga
     4261 cgctggatga agcgagttcc gccctgggcc tggccaagac gctgacggag aactcatcaa
     4321 ttcgccaaat gatcgagacc gtgcaagagg acttggttct cctgggaggg gaactggcct
     4381 gggagggcga cgaaggcaaa tatcggatcg gaacggacag gacccggagg ttggaggaat
     4441 ggattgaaac cctgcaaagg gaggtcccca ttcctcgaca attcatttat cccgggtgca
     4501 atccgtccag cgcggccatc gacctggcca ggagcgtcat tcgtcgagcc gagcgccggg
     4561 tagtggccat gaaggagcag gggctgattc gggaccccga catccatgcg tatctcaacc
     4621 gcctggccga ctttctgttc accctggcac gctatgccga gaaacggggc tgagaggcgg
     4681 gccgccagaa catccgggat gggaatccgt cgggatttct cctccatcgg cgcacccttc
     4741 gggggaagga gaacctcgaa gggaactgca tgaaaagagg tgagctgccg gttccgggcc
     4801 ggggagcgtg ccgccgccgg gtcgctcttt ttcgagttgt tttcacgaga cgggatggtt
     4861 tatatagaag agtttcgttc gttgagctga ctcccgggtt tgtcggggag ttccgagccg
     4921 ccctaaagga tgggagttcc catctctttt ttttaggatt gatccttagg aggatttgcc
     4981 ggggctcccg gttgcaggcg agtgcgggtc gcagagttcg ggtcgatcct ttgttttgaa
     5041 atcaagaatc catggaggag gagccaacat ggctcgagtg acgattgaag actgtctcga
     5101 aaaggtggag agccgctttt ccctggtgca cctggcggtg cgcagggtcc tgcagatgcg
     5161 cagcggggca cccctcatgg tgaacgctcc caagaacaag gaaatcgtcc tggccttgcg
     5221 ggagattgct gcggggaaag tcaccccgga gaacatccga gagctcgagg agtccaaggt
     5281 ccttgccgaa cccgcggtgg aggagaaaga ggaggtcacc cgccgggagg tgaaggaaat
     5341 cctggaggcc gcaacccagt acgacgcatc cctcgaatac gaagaggcgg ccccccccct
     5401 ggaacccgat gaagacgagc tctaagcgga tggggtggcc cggtgccttc catgacccgc
     5461 accttccgca ttcccggaag tctgtccggg gaatatgccg gtttgccggg cctcctgtcg
     5521 gcagcggatg cagaagcggc ggcggggcga aaggtggttt caatgagtag acaagaaatt
     5581 ctgtcctgtc tcgaaaagcg ggatttgctg aaccagccgg ccgtggccgt cgataagctc
     5641 cttcactggg gccgggtctt cgaggaagcc gagatgctga gcgatgcctt ggacttcttc
     5701 gaaaaggccg acagccggga cgacctgttg cgtctcttcg cccgggcgag ggaagagggg
     5761 gacctgttcc tgttcaggcg cctggcgaag gccttgaatc atgagcccac ctcggacgag
     5821 tggctgtccc tggctcgcag ggccgaagaa attggaaaga cggcctttgc gttggaagca
     5881 tatcgttcag gagggcacga tccctcgaaa ggggaatcgc cggaaggttc ccccgttcct
     5941 acctgaaaaa gggacgcttc attttccctc gaaggaggaa ctcgacacct gtaaaacggg
     6001 attggtcgtg agtctatgga gagtcgtcac ggttgacaag gcatccaagg gtggttagga
     6061 aattgatggc ttgccggcaa ccaatagacg gatggaacca cacgggagag gaaatcaagg
     6121 gtccatgaac aagcgggact actatgaaac tctgggtgtt tcgcgaaacg ccggggaaga
     6181 agaaataaag aaagcctatc ggaagctcgc tctgaaatat cacccggacc gcaacgcggg
     6241 ggacaaggag gcggaagaac gcttcaagga ggcggcggaa gcctacgaag tgcttcggga
     6301 ccctcagaaa cgccgcatct acgacaccta cgggcatgaa ggcctccaga acagcgggtt
     6361 cagcggtttc aggggatttg acgagatatt cagttccttc ggggatattt ttcaggattt
     6421 cttttcattt ggcttttcgg gtacccggcg aaccaagtcc gccgcgcgtc ccggcaacga
     6481 tctggtctat gacatgaccc tcacctttga agaggcggtc ttcggtacgg agaaggatat
     6541 cgagatcgag accctcgtgg agtgttcagg ttgtgagggg agcggagcgg cgccggggac
     6601 ccaggaaagc acctgtccga tgtgtcatgg gagtggacag gtggtgcagt cgcaagggtt
     6661 cttccgaatc agcaccactt gcggtcgttg tcagggaacg ggcagggttc tgacctcccc
     6721 gtgcaaccag tgccggggcc agggacgggt gaggcaggtc aaacgggtgc aggtgaggat
     6781 tcctgcaggc gtggacacgg gcacgcagct tcgcttgcgg ggagaagggg agagcggcta
     6841 tcgcagaggt cagcccggcg atctgtatgt gcggttggaa gtccgccccc acgagttttt
     6901 tgaacgggat ggggacaacc tgtacgggaa ggtgcccatt tccttcgtcc aggctatcct
     6961 gggggaccag atcgaggtgc ccacattgga cgggaggaag gccatccagg tcgaaccggg
     7021 aacccagccg ggtcacgtga gccgttttcc cggggaaggg gtgccccgat tgagaggaaa
     7081 tggacgggga gaccttttca tcgaactgga ggtgaccatc cccaaacacg tcggagaacg
     7141 tcaggcggaa attcttcgcg agttcatgga tttggaagcg gccaaggaag gccggaaggt
     7201 taagaagtgg ccgtggagcc gccgcaagga atccgattcc gacccggtgt tgaattctca
     7261 tcgccgggct cgcagttgag tgcagctcag tttcggggtg ggaggtgaga tcaagtgcgc
     7321 aggagctctg tggggcaata cgaagacttt gtggcttcgg ccttgtattg cccgcggtgt
     7381 aaatgtgcca tgccggtgcg aagccgcctt cttctggttc tccccgacgg ggagcttcat
     7441 gagtacctgt gccaggcttg tgcgtcttcc ctgggcacca agaccgtccg gaacaaacag
     7501 cagacccgca tttacttgcc ttgagctgct tgcggtgttt caatcttgca ccgcaggaaa
     7561 tccctgaagc tatgcagccg tgtacctgag ttgagcgatt ccgagagcat cagggcgtca
     7621 tgcccgcgaa ggcgggcatt cagtgggtct gcatgctctt tgcggattca cgcatgcgcg
     7681 ggatgacgaa ataggattcg taacggtcaa atcgctcaac ccagggtgtg gattgaaagg
     7741 acgccccttt gaacggggag ttgcgttgcc cgggcgggga cgtcccggag acgggtctta
     7801 gacaacagag gattggagac tatgattgag acggaaggct tgaccaagtt ctatggtcct
     7861 cttgcggcca tacgggatgt gaccttccac atcgaacggg gagaggttgt gggttttttg
     7921 ggccccaacg gggccggaaa atccaccacc attcgcattc tcacctgtta catgccggcc
     7981 acggccggaa gcgccaggat tgatgggacg gattgtttcg aacagtccct ggaggtccgc
     8041 agaaaggtcg ggtatttgcc ggaaaacgtt cccctgtacc tggatctgac tgtgcggcgc
     8101 tttcttcgtt tcgccgcagg ggtcaaggga atcaacggga aagatcagga aggggaaatc
     8161 tcaagggtca tcgggatctg cgggttggaa aaggtgtccc accggatctt gggtcatctt
     8221 tccaagggct accggcagcg cgtgggtctg gcccaggccc tgctcaacag cccgccggta
     8281 ctgatcctgg atgagcccac catcgggttg gatcccgctc agatcgttga gatccgcaag
     8341 ctcatccatg aactgcggga agaacacacg atccttctga gcagccacgt gctcccggaa
     8401 gtcgcccagc tgtgcgaccg ggtgctgatc atcaacaagg gccaaatcgt tgctacggac
     8461 actcccggca atctcacggc ccagttgcag aaatccgccc agatcgtcct gcaggtggac
     8521 ggcgatgcgg cagggcttgc tccggcactg gagagcctcg agggggttca aagagtcagc
     8581 ttccgggaaa accagcacgg gcgcttttgg atcgagacgg atcgctctcg ggaactgcgc
     8641 cccgagattg cccgattggt tgtggacagg cgcctggatc tcctggaact gaaattggtg
     8701 gacctcagcc tggaagagat cttcatgcaa ctcgtcaccg aagaaccatc tcaggaggag
     8761 tcggagacat gaaaggcttt gtgcccgttt accgaaagga gctgtacagc ctgttcgcct
     8821 cacccatctt ctatgtggtg gctttcacct tcctggtcat tgcaggctat tttttctaca
     8881 gtgccgttgc ctattacaac cttatgagct tccaggcaag ccagagcccc atggcggcga
     8941 ggcagctgaa cgtctcggat atggtgttgc gccccttctt cctggatctc agtatcgtcc
     9001 tgctgctcat ctcccctctg cttaccatgc ggctctatgc cgaagaacgc aagacgggca
     9061 ccatcgagct gcttcttacc tatccggtcc gcgatctttc cgccgtgctg gcgaagtttg
     9121 ccgccgtggc cacgtctttt ctggcgatcc tggcggggac gcttcccgga atgtttctgc
     9181 tccacatgat tgcggacccc agttatccca ccatcttttg cggctacctg ggggtgttcc
     9241 ttatgggcag cgccttcctc tccttggggg tctttacgtc gtctctgacc cagaaccaga
     9301 tcgttgcggc ggtgctctcc tttggggcgc tactcatgtt ctgggtgatc gggtggacca
     9361 agaaccttgt gggccccacg gcggggagct tcatcgacta tgtgtccgtc atccggcacc
     9421 tggacaattt cgccaagggg atcgtggaca cccgggacct cttgtactac ctgatgttca
     9481 tcgtcctttt cctgttcctg actcttcgac agatggagtc tttccgttgg aggggatagg
     9541 aggattgcgg cacggctttc cctcgatgcc gaggctcggg tgggacggag ttcctccggt
     9601 tctttggccc cgggacccgc aagaatcttg atccgtgatt gagtggaggt ttcgcgacat
     9661 gttgaagaag aaaaaaaccg gtcgcgcatg gagctacggt tccggcacgc tgatatcaac
     9721 cttgtttttc ctgggcatcc tcgttgtgct ggctctcatt gccgaaaaaa atccccggcg
     9781 gtgggacctg accgaatcca agagctacac cctctcggaa cagactcgaa aggtgctgga
     9841 ttccatcgac cagccggtga gcatcaaggc cttctttgcc acggccgctc cggagcagcc
     9901 caaggtcaag gacctgctgg atacctacgt gtactacaac ccgaagatca ctgcggaatt
     9961 catcgatccg gaccggcagc cggaagttgc aaagcaatac gagatccggg cctacggaac
    10021 catcatcctc gagggctacg gcaagaaaca acccttgccg accgccaccg aggaagggct
    10081 caccaacggc atcctgcgcc tgacccgcaa aaccgacaag aagctctact acctagtcgg
    10141 acacggggaa cgttccttcg aaaataccgc caaagagggc tactccacgg caaactacga
    10201 gctccagaag gcgagctacg ccctgagtcc cttgaacctc atgcagcagt cccaggtgcc
    10261 ggaggatgcg gtggcggtct tcgtgatcgg tccccaaaag aatctttttc cccaggaggt
    10321 ggaaagtctc aaggcctacc tggaacgggg cggaagggcc ctgatgtttc tggatcctta
    10381 ccgcgacggg ggattgcggg agctgatcgc aggctacggc atcgaactgg gtgacgacat
    10441 ggtcgtagac aagttgagcc gggtcttcgg gggcagctat ctcatgccgg tggtcatgga
    10501 atacggcggg cacaagatca ccaaggactt tgaagtggct accttctttt ccgaggcacg
    10561 cacggtacgc cctgccaagg aacctcccgc caacgtgcat gtcgaggccc tggcttccac
    10621 ttcccccaat gcctgggggg agacggacct ggatctccta caaaagggcc aggcgggctt
    10681 tgacgaaggg aaggacacgg ccggacccgt gtccatcgtc gtaatcgcgg aaatcgcctc
    10741 ccagcaaaag aaaggggaag ctcaggggaa aggtccagcc caggaaaccc cgaccccacc
    10801 cgacggcagc aattccgatg attccgaccc ttccggggat gcgggcagga agggatatct
    10861 gatcgtggcg ggagacgccg atttcgcgga caatacctac ttcggattgt ccggaaatgg
    10921 ggatttcttt ttaaacatgg tcaatttcat gggcgaagat gaaaatctca tcaccatcaa
    10981 gcccaggcag aagaccggca cggcccgtct catgaccccc ggtcaggccc gcacaatgtt
    11041 cctgacgctc ctggtctttg tgcctctcac cgttgtggcc tgcggcttgt tggtgtatcg
    11101 agtcaggagg tctcagagat gaagtggcgt cactcgctcc tctacttact ggttttctgc
    11161 gtcgttgccg ggtacttcta ctacttcgag gtggtgaaga acgagcggaa agcggcggcg
    11221 gacaaagagg ccaagcgcct gttccaggtc gcccgggaaa gcctcgatgc cgtcgagctt
    11281 catccaaagg ggcagaaatc catcgtgctc aagaaggaag accggtggaa gatcctggag
    11341 cctgtgcagg ccgaggcgga tcgaacggct gtggacgacc tggtgagtac cttgagcaaa
    11401 ctcgaatccg aacgggaagt gattgcggct gcccaggatc tgacccccta cggtctggcc
    11461 gacccgtccc tcaaggtccg attccattcg caggagacgt ggatggagct cctggtggga
    11521 gaaaagaatc ccacgggcag cggctactat gtcaagaagg gggaccgacc ggaggtcttt
    11581 ctcatcgctc acagcgactg gggacccatg agcagagggg tgaacgacct gcgcagaaaa
    11641 cagctcttca cctttgaacc gggaaagatc gtcgggctga ggcttgcctg gcaggacggc
    11701 agcatcgtcg atgtgaagaa gcaggacaag ccccccgggt gggaaattgc gggggagccc
    11761 gaggtgagcg tcaaggaatc gaaggtccgg aatgccgtgg aacaggtcca gtggcttcgc
    11821 gcccgggatt tccctccgga gggccccgag gacctcaagg cccacggcct ggactctccc
    11881 ttcctgcggg tggatgtgga tttgggagag ggacggacgg ccttcctcct ggtagggcag
    11941 aaggatcagg acggcaaacg ggttcgggct tatagttccg agctcggtgc cgtggcctgg
    12001 atcgaggcaa gcctcctcaa cgaattgccc aagacgcccc gggacctgga ggaccgatcc
    12061 gtggtcggtg tgaaacccga tgtcgtccaa aaagcggacc tgcgcctggg ggatctgcac
    12121 cggcaggccg ttcgtatgga tgacaagtgg ggattggccc gggaaggaaa aggtcccgag
    12181 gcactcaagg agccctggca ggtgcggtcc ctgttgtggg atttgaacca gctggagtac
    12241 gtccaaaaag tggaaccggt tcccccgctt ccccaaaacc ccttcgggcg gattgaatta
    12301 tggtcccaga cgcagctgct ggccgtgatg ggttggaacc aggacgagac aggccctgaa
    12361 tcgctccgga ccgtctgggt ggaaaaggac gggaacctaa aggccttgcg catgaaaagc
    12421 gaggacctgg agaaggttcg gaaggccgtc cagggcattc tttctccttc agataaggaa
    12481 gcttcctcct gaccttatcc cgaagaggaa tccctgccgg tgactctccg ttccacggtg
    12541 cttccggcgt ggggtgaagg atcccggctt tggcggaccg tcgcctattg ctgcgggggc
    12601 gggccatatg ccccccctcg aagcggtcgt ggtctcttct ctttggcgac ttgattccgt
    12661 aggaatcctg ctcctgtcga aactcccgga tctcggccat cctccatggg tcgagaaggc
    12721 ttgcggcccg tagggtcggc cccccaaaag cacccgggaa cggaagcaga cgggccccgt
    12781 tgtgcttttt ttgtgttcgg gtctgcgggt cttgtgctaa atgaatccca tggaccgatg
    12841 gagattgggc tgtgtattga cgtctaaagg aggaagctat gaaacgcaaa gccacgtacc
    12901 gtctaatggt tacacgccgg gaaatgctgt atgtgcatga tgaattcgat cattcgctca
    12961 tgttggtgga gatggagggc gagcccatcg attaccaggt tggagtggcg ggagagtttg
    13021 tctcccggcg cagtgtgacc tttcatgatc gaattcgggg aaacggcccc atgaagggct
    13081 atgccatcac gaatttccga gaaggctccg tctgcacctc ttatgagggc gccagggaag
    13141 gagacctcac caaggggacc tggaaggcct acaagggaat cggaaagctg gccaatctca
    13201 agggcaacgg caccttcacg gtgaaacccg gccagaggga ccgggagtac atcctggaag
    13261 tcgagggaga ttacgacctg taggatccct gatccattcg cttccgccaa cgagctcggg
    13321 ctgttctcgt ggattccctt ccttcccgga tccgatctcc attcgacaga accgctccca
    13381 ttgccttgcg cttccccgaa cagattgccc ataaagagcc gagaggcggg tctcccaacc
    13441 gtgggagacc cgcctctcct tttcttgcgc taccggaatg gagcggcgcg tttgatcctt
    13501 ccgagagccg ctcaggggtg cctcctacgg aagcttggca gcggttttgg ccggggagat
    13561 gttcaaatcg tagctgaaga tcgtgctttt ggattcccac aatttcagaa ggtccctggc
    13621 catgatcagg ctggccacca gttccgtggt gccgtcgttg ttgaggtccc cgactcggat
    13681 gttgctgatc atcccgttga cttcacgggt cttccagttt tccaccattc ccatctggtc
    13741 ccaggacaag gacacgattt ctccccgttc atagtgcttg aggccttcgg gcaggaatcg
    13801 ggtcagcaca tccaagctgc gggtcaccac gatctccggg atgttgtcct tgttgaggtc
    13861 ggtgacgaca atgttgctgg gaatggcata catatccacc tggttgaagc ggcggtccat
    13921 gaccttggct tcgaaggagt tggtcgtggc ggcaaagtag cgatcgccct tccataactg
    13981 gtctcccgaa gcgctgagga ccaccaggcg gttggcgctg tcgatgaaaa gggtttcctc
    14041 catcaagtcg ccgttgatgt ccgctttggc gaaattgaaa acgttgcagc ggcttgagac
    14101 cccaacgggg gaagagggcg caaggccctt gcctcggagt tgcatctcga aaataccggg
    14161 tttgaaggcc ccttcggtga tttgcccctt ctcctggccg atcaggactt ttcctctctt
    14221 ggggaaatcc actccgttga gaaagtaacc gacccgatcg caaactacct gcagctttcc
    14281 ctggtcgtag ctgaggccga aggaagccag gtcctcgacg gcacctccgc ccccgcccat
    14341 ggcggattca ccgtagacgg gcctggaagt gttgtatttg cggaggttgg aaacgtagat
    14401 ctcggccctt ccgtcccgat tgaggtccac cagggcaacg ttgaggaaac ggtccacctt
    14461 ctgaccgttg aaagtcccga tggttttcag accctggccc tccctgcgat acacggtgac
    14521 cttgttcatc gtgaccgtaa cgatctcgtt ctttccatcc ccgtccacgt ccccgaggtc
    14581 catgcccacg accccggcgg taatggtctg gctacgccag agacctccct gtcggaggga
    14641 tccctcggga gtgacttcga taaaattggg attgagatag gaaattttgt cgcccgggag
    14701 catggtttcg ggaaccagga gttcggggtt cctcaggccg gcaaattccc tgtcctcgcc
    14761 ccccgcggca tccgctcgct gttccccggg gcgggcgaag accttggcgt tgatgtcctg
    14821 ggcgaactgg ttgatcttgg gaatcacctc gtccagagtc ttggtttggg cataaagatg
    14881 gatcggctcg gatttcccgg caaccgacgc catcttggcg tcgatgctga tggattggcc
    14941 cagggcggtg atgctcccaa agaggacata gtcggctttc aagttccggc cgatgcgaag
    15001 ggcctcctcc agagggagat cccccttgct gctgggaagg gcctgagtga cggccgttcg
    15061 gtccaggacc tgcacttttc cctgccaggc gagccgtgag gacagcatgt cgcgaatccc
    15121 gctctgaagg taggccagct ccgggggagt gtgcatggaa aaaggaagga cggccacctt
    15181 ggcggggacc ccggccggtt cggcactcca gcagggagcg gtacccagca cgacaagaca
    15241 aaggattgac caaatcctga ttttggcgag acggcacatt ttcatgggtg attctcctct
    15301 tgacacctat gatgacgata tgaaatccgt aagtcttccg cttctcacga ccgttcctga
    15361 aggtgcaagc atactacaat gcaagcacct ttgggaacac gaaaatggcc cgggcaaaaa
    15421 ggcgggccgc agccgggccg ctgggttttt cgaatcggat aagaggaggc gctggaaggt
    15481 cgaagtcggc gtgctcctaa cggaaaacga ttccgcagaa aaatgaaaga catgcggcgg
    15541 atttgtcttg acatccttgg accgggtgaa gtagacgtaa tcttcaaagt gtctaagggg
    15601 cgaaaccgtt aacctatgag agaaaaaggg ggtgaaaaca gaccgttgaa aatcaaaagg
    15661 actgaagtga agtttcacgg aaagttagct ttttaggagg aaagagatga aaaagtactt
    15721 catgatcttt gtggcgttgg ccgtggcggt gaccctggcc tcccccgtgc ttgcggcggt
    15781 agaattcaag tacggcggcc agttccggaa ccgcatcgac agttccgaca acgtttacga
    15841 cggcagtgac aaaggcagcg acaatctcaa cttcattgat cagcggttga gactgtactt
    15901 caccttccag gcaagtgaga acctgaaatt ggtctggaag atggaagtgg gcgacaccac
    15961 ctggggtaac gactggccga cggccgggac ctccaccttg ggcaagggcg gccgcgtggg
    16021 ttcggatgct cagaacgtcg aaaccaagaa cgcctacatc gacttcaaca tccccaacac
    16081 ccccgtgaag atcatctccg ggatccagac cctgaacctg ttgaactcct ggatcgtgga
    16141 cgacgatttc tccgccatcg tggccaaggc caagtttgag ccgtttgcgg tgaccttggg
    16201 ctatgttgcg gctcagaact acgacctgtc tcactacgtg gacaacatcg actccttctt
    16261 ctttgccttc gactgggtgc agaagccctt cagcgcttca ttgatcggtt actaccagta
    16321 cggcagagac accgttgcca gcatttatcc gtggttccgt tcacccacga ataatgtggg
    16381 caacagcagc agcagcgcta cgttcttccc gtactacgga agcttggccc cgggacgcac
    16441 cgatatgttc cgcaacggcc tgttgtccgt gggcgacaac aacctgttcg acttgggctt
    16501 caacttcagc tacaagatcg attggttgag tgcctatgtg agcttcgtga agaacctggg
    16561 cagcttcgat ctgaacgtgg ccaacaccta tccgtttgcg ccgcccagca cgaaccccag
    16621 caattacgtg aaggggacgt ccatggacta caccggctgg atggtggatg ccggcgtaac
    16681 gtactactgt gctccctgga cggccaacat cggcggcttc tacaccagcg gccaggaaag
    16741 ccccagccag caggtggtcg gcaacatctt caacggactg cctctcgatc cttacaagct
    16801 gtatttcatc tccctcgatt cctttaaggg taatgacctg gatcgattcg tgcttcccct
    16861 ggcgcagttc aagtacttct ctgaaatcat cggcggcggc gtcatcggcg acaccatcga
    16921 cagcggcaac ggatcctggg gcaatgtcca gaggggcaac ttctggcagg gattccccaa
    16981 cccgttcaac atttggacca tcactgcggg gggttcctat caactgcttc ccaagaccaa
    17041 gttgagcgct tcctactggt acttcgggac ctccgaggat gtgcccgtgg ccctctcgag
    17101 ggtgcttcgc gtacggccca acggcgatat cgtcgccagc tacgacatgg acaaccagat
    17161 cggccacgag tttgatgtgt acctggatca tgaaatcgtg gacggcctgc agttgacctt
    17221 ggtgggtgcc tacctggttt ccggggatgc tttcgccccg atcaacccga ccttaaacaa
    17281 caacatcaac acgattttcc ggaagaacgc gagcgatgcg tacgaactgg gtgccaggct
    17341 gcagtggaac ttctagttcc cgagcctgat cgccggtgcg cgtagagtga gcccgggcgg
    17401 ggaaacccgc ccgggttttt ttattgaacc tgtttgattt caggggggtg tgccgggggg
    17461 ctgccccgcg gattccctgc cgttcctaag ggggggcgtg gcatagggga aagattccct
    17521 ccgtgccccc ccgtaacata aatgtcgtcg agttaacagc cccttacgca atatttgtct
    17581 tgactcggct gcgcatgcca aagtaaaaga ttttctaatt gtaaatgggg cgaaatcctc
    17641 aacctatgag agagaaaggg gggaaaacag accgttggaa agcgaaaggg ccgaagtgaa
    17701 gattcagcga gagttggctt ttctaggagg aaagagatga aacggtactt catgatcctg
    17761 gtggctttgg ccgtggcggt gaccctggcc tcccccgcgc ttgcggcggt agaattcaag
    17821 tacggcggcc agttcaagaa ccgcatccac agctccaaca acacgtggga cggcagcgac
    17881 gaaggcagcg acaacctcaa cttccttgat caacgactga gactctactt taccttccag
    17941 gcaagtgaga acctgaaatt ggtctggaag atggaagtgg gcgacaccac ctggggtaac
    18001 gactggccga cggccgggac ctccaccttg ggcaagggcg gccgcgtggg ttcggatgct
    18061 cagaacgtcg aaaccaagaa cgcctacatc gacttcaaca tccccaacac ccccgtgaag
    18121 atcatctccg ggatccagac cctgaacctg ttgaactcct ggatcgtgga cgacgatttc
    18181 tccgccatcg tggccaaggc caagtttgag ccgtttgcgg tgaccttggg ctatgttgcg
    18241 gctcagaact acgacctgtc tcactacgtg gacaacatcg actccttctt ctttgccttc
    18301 gactgggtgc agaagccctt cagcgcttca ttgatcggtt actaccagta cggcagggac
    18361 accatcgcca gcgtgtggcc ctggacccgg tcccccttgg ggaacatcgg gaacagcacc
    18421 actagcgccg ctttcttccc gtattacgga agcttggttc cgtcggcgaa cgacatgttc
    18481 cgcaacggcc tgctttcggt gagcgacaac agcctgttcg atctgggcct caacttcagc
    18541 tacaagatcg actggttgag tgcctatgtg accttcgtga agaacctggg cagcttcgat
    18601 gtgaatatcg cgaaatccta tccgttgttg ccgcccagca ccaatcccac caactacacc
    18661 aagttccggt ccatggacta caccggctgg atggtggatg cgggcgtaac gtactactgc
    18721 gctccctgga cggccaacat cggcggtttc tacaccagcg gccaggaaag ccccagctcg
    18781 aaggtggttg gaaacatttt cagcggactg cccgtcgatc cttaccagct gtatttcatt
    18841 tccatcgatt cctttaaggg aaatgacctg gatcggttcg tgatgcccct gtcccagacc
    18901 aagtactcct cggaaatcat cggcggcggg atactcggcg atacccagga tcgggggaac
    18961 ggtcccttca ccggcgacgt ccagagaggc aacttctggg aaggctatgc ctcgccgttc
    19021 aacatctgga ccatcaccgt gggcggttcc tatcaactgc ttcccaagac caagctgagc
    19081 gcctcctact ggtacttcaa cacctcggag gatgttcccg tggcgttgaa gaagacgttc
    19141 gggattgctc ccaacggcga catcatcgcc cggtacgaga tggacagcca gatcggccac
    19201 gaattcgatg tatacctgga tcatgaaatc gtggacggcc tgcagttgac cctggtgggc
    19261 gcctacttgg ttgccgggga cgctttctgt ccgatcaacc ggaccctgag cgccaacccg
    19321 aatcagatct tcaggaagaa tgcagacgat gcatacgaac tgggtgccag gctgcagtgg
    19381 aacttctagt tcccgagcct gatcgccggt gcgcgtagag tgagcccggg cgggttttcc
    19441 cgcccgggtt tttttatggc gcgcccgatt gggtgacggt gtcgtctccg ggagcagcct
    19501 ttctcccaaa gcggaaatcg gaaaaaaatc gggttgactt ggattccctc gatcggtcgg
    19561 ttaccgtcag agagagaatt tttgcagcag acaccgcccg gaacaacagg agaagacgtt
    19621 gtatttgcga cgagtgcgcc ggggccgtgg gttccgctac atcctccggg aaagctaccg
    19681 ggttggggat gcctaccgac atcgggactt gatggacttg gggacaaatc ctgaagagta
    19741 catcgtctac ccggggggaa atgggtttta ctttcgcttc gaagtggagg aatcgttgcg
    19801 ggatcaggga atcgagtact cttcccggga cctggacgag gccttcctgc cctttcttcc
    19861 tctccatatt cgccgggtcc tgggaatgtt cgaccatggt acggggggag caagaaccaa
    19921 aaagctcgag aggacggaag accttcggca gctccaggag aaactgcatc ccttcgacaa
    19981 gcgtcggctg cactatcttc gttttgggca aatggagata ggggggtccc aggaccagtg
    20041 gttcaagttc ctccatgttc tgttccgaaa aagccgggac gaaatcgagc atatactgga
    20101 gggaatggag cagcggctcc gacccacgga gctgcgcaac tacctgtacg ccgctcttga
    20161 gttggccgcg tattttcccc accatcggat gaggcactac ccgggggccc tcgatcagga
    20221 gaaggtcgac gagtgttttc tcgaagagct ctgccgtctc aatggggacc cgtcgtatct
    20281 cctgggagcc gattccccgg gaggagaacc gctgcacccg tatctggtcc gatatctgat
    20341 cctctatttc gattccggag attgggcggc gaacccctat tggcagtacg cgcagcaatt
    20401 tgcacgtggg cggttttctc ggtcccggcc ctttcgtgca gagctggcgc ctcgagaagc
    20461 gtgccgggtc ttcggggttt cctatgagac gtttcaggcc atgacccgca aagaactgat
    20521 tcgggattac cgcaggaagg ccaaggagtc tcatccggac cggggaggca atcatgaagt
    20581 tttcgtgaag cttgccgaag cctttcggtg cctcctggcc aacaagccgt gaccccgggt
    20641 ttccttccgt caggccctca tgatttgttg actccccggg cgtcccgggt actcagtcct
    20701 ttcatgggta gttcttttac caagggggta tgggtcggtc tcccggctca cggtctttcc
    20761 caccatggat tccaagggta agacccgatt gaactccgaa caccggggtc cgggcatcgg
    20821 acacatgact cgcggcctgg ctgcgagata cctccacgga gaatcccctg tgaagagctc
    20881 gtgccggaag cggaactgcg caagcattcg gaatccaacc agattcccta ttttgtcgtg
    20941 gactgcatcg tggagcagcc ctggggggcc catcccagtt ccgtaccctt ttgccatgac
    21001 tacgatgccc cgttcctgcg caccatggat gcggcatcca ggaatcccga agacctcaag
    21061 gcctggttgg cagagccgca ggcggcgccg gccaggacag ggacccgata ggggcaacgc
    21121 cgcattggcg acgccgcagg cggtgccaac atggggggct accggtttga cgaccgggga
    21181 attctctacc tggataccct tcaccccggg gtgaccgtcg agaagatccg ggaaaactgc
    21241 gggttcgacc tcaacgtcac ccgggtgaag ggagagacgc ctgcgcccac gtaccgggag
    21301 ctcttcgtcc tgagagagtt cgtggacccg gaactgatct ttctgccccc aaagatggat
    21361 tacctcccag tcatccggtc cttccccttc atgaaacatt tcccgcctgc agcagggccg
    21421 aaggcggtat gggctctcca ccaaatcccc ccgtcgcccg tttcgatgac ctcgtactca
    21481 cccaatggat aggaggtgct tttcatcctt ctctcccacc gaaacaggca cgttgcggga
    21541 atgcccccac ttctcaccgg gagatgaatc ttcccggcgc cgggtaacgc caagttgttt
    21601 cacccatcgg acctccatcg ggcaaggtcc cttcctgcaa cggccggccc atagggattg
    21661 aggcggtccc ggccttgtcg aagccgtttc aatccatggt tcatccctca gatatactgg
    21721 tcccccaggt ccgtaatgag ctgttcgagg tcgccgccgg tttcggactc cagctccctt
    21781 ttggccgact gcaggcgttc ccgggtggtg ggatcctgca gttcgtcgag ggacctcagg
    21841 gcccctcccc ggcgccccag ccgaaacagg agcctttcct ccgggggcag ggccaggtag
    21901 cggtcgatca ccgccagcat ggcctctttg tcttcgggca gttttccggc cacctcctcc
    21961 aggaggttca tgatgtgatc cgaggtcaac acgctgtgaa tcccctccag ggattcgaga
    22021 aaccaccgaa tttcccggac gacgtcgtcg tctccc
//
LOCUS       DAOOVC010000057        21944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1616_length_21944_cov_7.070583, whole genome shotgun sequence.
ACCESSION   DAOOVC010000057 DAOOVC010000000
VERSION     DAOOVC010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 21944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21944
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1616_length_21944_cov_7.070583"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /gene="groEL"
                     /locus_tag="PLM79_09155"
     CDS             <1..1005
                     /gene="groEL"
                     /locus_tag="PLM79_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV86517.1"
                     /translation="ERMEAVLEEPYILACEKKISNMKDLLPLLEQIAKMGKPLLIIAE
                     DVEGEALATLVVNKLRGTLKVCAVKAPGFGDRRKAMLQDIAILTGGQVISEDLGIKLE
                     NVTLKEFGRCKTVRVDKDNTTIIDGAGTREAIEARIKQIRTQIEETTSNYDREKLQER
                     LAKIVGGVAVIKVGAATEVEMKEKKARVEDALNATRAAVEEGIVPGGGVALLRCIPAL
                     AQIDGDGAEAFGVNIVKRALEEPVRQIANNAGVEGSVVVERLKGENGAFGFNAETERY
                     EDLVEAGVIDPTKVVRFALQNAASVAALMLTTEAMVAEKPKKKEPAAPVPHGGMEDMY
                     "
     gene            1028..1219
                     /locus_tag="PLM79_09160"
     CDS             1028..1219
                     /locus_tag="PLM79_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86518.1"
                     /translation="MDFDRAMRVFRLYCKQWKENPVQAESLLWTEEERLTIREIKDLI
                     KECAGLFEADEPLPLAKAV"
     gene            complement(1504..2736)
                     /locus_tag="PLM79_09165"
     CDS             complement(1504..2736)
                     /locus_tag="PLM79_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV86519.1"
                     /translation="MTTDSIIRFAPRMDQLPPYLFGMINKMRMEKRWRGDDVIDLGMG
                     NPIDPAPEAVTRKLCEVVQDPKSHRYPVAMGLRNLRREIAKYYDRDYGVSLDSEGEVI
                     CTIGSKEGISHLCLALIGPGDTVVVPTPAFPIHIYAAIIAGGNVIQVPLANEEVFLER
                     ISTVCQALVPTPKVLMLNYPHNPTSVVAGRELFQEVVKLAKRYRFMVVHDFAYAKITF
                     DGYVAPSFLEVPGARDVGVEFGSFSKSYNMAGWRIGYCVGNREIIQGLAKIKGYYDYG
                     IFSAIQIAGIVALRDCEPAVHQQAKIYESRRDVLCKGLRRMGWPVEKPRAGMFVWTKI
                     PEPYSQMGSMAFAVKLMEEANVAVAPGIGFGEEGEGYLRLALVENEHRIRQALRQMGK
                     VLRGLNPAPGSEEETASA"
     gene            2857..3102
                     /locus_tag="PLM79_09170"
     CDS             2857..3102
                     /locus_tag="PLM79_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86520.1"
                     /translation="MASVVSEGWSLHTPTGTQADLNAAAAANHGNKVILLIFAQPFSQ
                     LLKHVSNSPENVNEQSPAASTPPGKSGVPGRCIREKA"
     gene            3452..3901
                     /locus_tag="PLM79_09175"
     CDS             3452..3901
                     /locus_tag="PLM79_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HOV86521.1"
                     /translation="MVPFSRRLLWFWLLPLWIGAAAVLAVSAQEPAQEAPGESATAKL
                     ALVQSSMCEKVENLAPVRPAIAFSVSVGQVYCFSAFEPVPQPTLIYHRWYHRDELSTQ
                     TRLRLYPPKWSTYSVIQLRETDKGAWRVEITDQDGRVFGVLRFSITD"
     gene            3922..5373
                     /locus_tag="PLM79_09180"
     CDS             3922..5373
                     /locus_tag="PLM79_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HOV86522.1"
                     /translation="MNKFLTFLSTVRWQDLVDIVINSYILFRVYVLFQGTNAFRVLVG
                     ITSLWILQEVAASMGLILTSWAVRGITAAAAIIIIVVFRNEIRSVLQVRNLKAFLWGS
                     PRREQQAPVEVIVDSIYQLGKKRIGALIVFPGKEDLAEVVHGGIQWDGTVSREMLYSI
                     FWPKNPVHDGAIIIRDNRVAEVGVLLPLSQRQDLPSYYGTRHRAAAGLAERTDALIVA
                     VSEERGRVTVAQGNFIKPVAQPEELSHLLKKHLNFQDRINGHEHKRETLKLILAGVTS
                     LLVMTGIWFGFTRSQDTVIALNVPIEYVNRPPIYEIMDTSVDEIRLQLYGSSALIKSL
                     RAGQVQLKIDLSKATEGRNVFPITQENIILPPGIFLNKVQPTSIEVILDVPVTKELPV
                     QVDWTGKLPEHLMLVSAAVTPESLKVIGGGKILEKAGTIYTTPVRLDHLTKSGTVTAS
                     IVLTPPSLKLASGSSDRVTIHFRVQERPKADRR"
     gene            complement(5418..6779)
                     /locus_tag="PLM79_09185"
     CDS             complement(5418..6779)
                     /locus_tag="PLM79_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86523.1"
                     /translation="MIISNDILEKTCKDMIETILFCLSDATKGTIYRIGKMPNLQAVR
                     ITSGIRREASDEIDWGLPEVSDYNAPGKSWIEYRDRPGHVLEAMGWCVERQKSWTADD
                     PYEDIRSVRKQLSGEIEDFHHMEPVLVPKRYLYGDQLGTLVYPLDCAGRPIWQDSEYV
                     VAAVVKIHFKPYAIHRGDRSTKLIKKLSRTLGTELLSLHIRETLAEAQKDLTRQRLRS
                     CNVLAHDLRNTLVKLGFICTAINTEFGYLREQWEDQVEKALPEPGTRKALLDRLTRLL
                     ESRLPSLNGAEDLLRLGRTLVREQKELAHLSPMPAAGLKWLEHQIRPKWMRLLSESDA
                     WSADREEILEVLERYQHSSWLGMSEELLEKMQHLPEEIRREWPRLAYVDFTADKLRTL
                     NDAIRFLDHPQLSIPHKHQTRKILASLRAIVEMIPEIEERSNRIISSLRNGGASDIDP
                     CTP"
     gene            complement(7060..8559)
                     /gene="malQ"
                     /locus_tag="PLM79_09190"
     CDS             complement(7060..8559)
                     /gene="malQ"
                     /locus_tag="PLM79_09190"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOV86524.1"
                     /translation="MKNRRSGLLLHITSLPSPYGVGDLGPEARRFADLLAEAGQSLWQ
                     VLPLNPTLGIYGNSPYSSPSAFAGNPLLISPDDLVRDGILDPSDLEDPPAGNVYRTDY
                     PRAAQYKERLLRKAFDRHRDRLEDDPTFLEFRRRNGHWLEDYSLFRALKEEFRETAWG
                     DWPEEIRDRHEAPLRDARLRLSDAVLEHQFVQFLFFTQWRSLKEYCNKRCIQIIGDIP
                     IYVSYDSADVWAHPRLFKLDERRRPEFVAGVPPDYFSETGQLWGNPVYSWKENRKTDF
                     RWWLDRMEHNLCLFDLIRLDHFRGFVSYWEVPGSQKTAIGGKWVKVPTRQLFDRFFRR
                     FASLPIIAEDLGVITADVREAMRACAFPGMKILVFAFGGDVAQNPYAPHNHSPDSIVY
                     TGTHDNNTVKGWFCREAGPGEKESLFAYLGRGIGEEEVAWELVRLGMMSVARMAVFPM
                     QDILGLGEEGRMNLPAVTHGNWEWRMSSEQFSPELVQRLRAATRLFGRC"
     gene            complement(8696..10618)
                     /gene="glgB"
                     /locus_tag="PLM79_09195"
     CDS             complement(8696..10618)
                     /gene="glgB"
                     /locus_tag="PLM79_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOV86525.1"
                     /translation="MTQEVWYRGSMLTDHDIYLFKQGNHFRLHEKMGAHILTVEGVRG
                     VHFAVWAPNAERVSVVGDFNQWDPESHPLDVRGDGSGIWEGFIPGLEQGATYKYHIFS
                     KSNGYRVDKGDPFAFFWETPPKTASIVWDLDYPWEDSHWMSRRREANALNAPHSIYEV
                     HLGSWRRVPEEDNRPLTYREMGDYLVSYVRDMGFTHVEFLPVMEHPFYGSWGYQTVGY
                     FAPTARYGTPQDFMALIDRLHRNDIGVILDWVPSHFPSDEHGLVYFDGTYLFEHADPK
                     KGFHPEWNSYIFNHGRYEVRSFLISSAIHWLNRYHADGLRVDAVASMLYLDYARKEGE
                     WIPNPYGGRENLDAIYFIQRFNQAVYETQPDVQTIAEESTAWPMVSRPTYLGGLGFGM
                     KWNMGWMHDTLEYFSKDPIHRKYHQNQLTFNIWYAFFENFILPLSHDEVVYGKGSLLR
                     RMPGDDWQKFANLRLLFGYMLGQPGKKLLFMGGEFGQWDEWYHEKSLDWDLLEYPFHQ
                     GVQSWVRDLNRFYRTERALFEDDFSSQGFEWVDFHDADASMIAFLRKGKTTKRVVLVA
                     ANFTPVPRSDYRLGVPTGGFWEEVLNSDAEIYGGSGMGNSGGVHAQPTASHGRPYSVS
                     LVVPPLGVVFLRSTEP"
     gene            complement(10961..11443)
                     /locus_tag="PLM79_09200"
     CDS             complement(10961..11443)
                     /locus_tag="PLM79_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOV86526.1"
                     /translation="MVEVSELRKFDLFEAFSDAQLEKIAGVTQRKSYKANGRVYESGE
                     PARYLFVVEKGLVSLREIKPEDQIGIAFEMRQRGELFGTACFTDQRFYTLTGVCLEDS
                     DVLAVDADKLLEMCAADPELGYKLMKKITQVYFERYKVAKRQLHEMVKTPSIITALPG
                     "
     gene            complement(11507..12268)
                     /locus_tag="PLM79_09205"
     CDS             complement(11507..12268)
                     /locus_tag="PLM79_09205"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HOV86527.1"
                     /translation="MEMNRSLHGQTAQHRAHYSQAGNAEGIAVYTLHNAENRSLTPDH
                     LCLFFDSFPDLCSSIAPVLTTSLLAGDQVLCVLHKRGRSRLIDYLRGTGWDIDPNLFS
                     RQLDFEKAEEFFLTAGSFSPHAVLDRLGGRIDEALSRGYPNLFLIADMGWILGGVRGS
                     ECLIEYETELNGFLKGKKCSILCTYGLKEFTPAILLYVLVTHPKLVGRSSICNNLYYG
                     TSPCLLTGDTPSNILGRWLQKIEESGPSRRGPRSV"
     gene            complement(12400..13077)
                     /locus_tag="PLM79_09210"
     CDS             complement(12400..13077)
                     /locus_tag="PLM79_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV86528.1"
                     /translation="MARKTTILVVDDHPLFREGIKSLISRNPSFQVSGEAGDAAEGLR
                     LAGELRPDLVVVDISLPDKSGIELTRNILAVLPQTRVLMLSMHAKIDYVTESFQAGAT
                     GYVVKESAADRLVAGLEAVSRGEFYLDCSLSSRVVERLVDLPRKEAKVTDRKYGTLTP
                     REQQVLRLIAEGLSKKEIAANLCISSKTVDNHRARIMEKLDLHSTLELIRYAARLGLI
                     DIDRWKE"
     gene            complement(13070..14548)
                     /locus_tag="PLM79_09215"
     CDS             complement(13070..14548)
                     /locus_tag="PLM79_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86529.1"
                     /translation="MIRKDNPSDIGEAEALSLVVIEDEEEHFRLMKRVINKDLPHATL
                     HHFRDCSECLEQIDRIQPDLIVTDDRMPGMNGIQFLEFLNERRHNIPVIMITGREDEK
                     IAAQAMKLGAWDYLVKSADCFALLPRLLEKVVREKRLRESLKETERRFRDLAERTSDW
                     IWEVDTHGRYTYSNPLVEAILGFTAEEVLGKHFYDFSAPQERESQKKEALPLGDQGRP
                     ISGYVKRLVHKDGHEIMVETSAVPIRDPSGALVGYRGIDRDITLRKRAEEHIRYLTRQ
                     LMSAQEMERQRLSADLHDVIMQDLSTLKIGLDTLLDGTEEAHPSLRNRVAELSKMLQE
                     SIEALRGLAYHLRPPTLDQLGLVRTIHRTCEGFARRNGIEVDFVAGGIDDLHLGSETE
                     ITLFRLIQEGLSNVKKHSKATRVAIRLVASYPKIILRIEDNGTGFDVESRLWSSLNER
                     CMGIRSMEERVALLRGRMKIDSRPNHGTRIRIEFPLQEKGVG"
     gene            15260..15952
                     /locus_tag="PLM79_09220"
     CDS             15260..15952
                     /locus_tag="PLM79_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV86530.1"
                     /translation="MIWQGMVEAGRLLATLDADVIHITLRTLQVSVTATLISILIGIP
                     AGTALALTNFWGRNFVVSVVNFGMGLPPVVVGLITWLLLARYGPLGSLSLLYTPTAIV
                     IAQAIIASPIVAGFSLAALQSVNPKLRLQILSLGANRFQYFLLLLWEARLGILAAIIA
                     GFGGVISEVGASMMVGGNIRGYTRVLTTATVLEVSKGKFELATALSVILLVLSFFVTA
                     SLSYLQQRGRRA"
     gene            15949..16668
                     /locus_tag="PLM79_09225"
     CDS             15949..16668
                     /locus_tag="PLM79_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86531.1"
                     /translation="MNPVIAAHNLKVVRGGKLILRVDELAVEKGQVLAVVGPNGAGKT
                     CLLHTLALLEPPASGTVRLFDVDAFNGNQLALRRRMALVFQEPLLLDTTVKRNITLPL
                     RMRGISGREANARSDRWLHRFGIGSLSARSARALSGGEAQRTSLARAFAMEPEILLLD
                     EPFTALDYPTRKSLVDELGEVLKEMNLTTVFVTHDYSEIPPLTEQVAVLFDGRIVKRG
                     SSREIFGEDFLKKKSYAPWET"
     gene            complement(16718..17467)
                     /locus_tag="PLM79_09230"
     CDS             complement(16718..17467)
                     /locus_tag="PLM79_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86532.1"
                     /translation="MSSTRKIIEINEERCDGCGQCAVACAEGAIEIVNGKAKLVSDVY
                     CDGLGACLGECPQGALRIIEREAGEFDPAAVEQYIEERKKAREAQHPAPACGCPSTMI
                     RVMAPKPAGADLSSGSQRIPSALTHWPVQIRLVPAKAPFLKGAHLLVAADCTPVAYPD
                     FHRDFLKDRVVLLGCPKFDHAPEYIKRFEEIFREADIQSVTVLDMEVPCCSALPVIVK
                     RGMDAAGKKVPLEEIVLGVGGEILRKNLSAG"
     gene            complement(17566..18309)
                     /locus_tag="PLM79_09235"
     CDS             complement(17566..18309)
                     /locus_tag="PLM79_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV86533.1"
                     /translation="MKCPGQDSRFWDENAIFEVKCPECGHEVEFFRDETRRKCRHCGH
                     TVTNPKMDFGCASYCRFAAQCLGELPPELTAQRQELLKDRVALEMKRYFKQDFRRIGH
                     ASKVARYAERIVKEEKGDPAVVLMAAYLHDIGIKEAERKYNSTGARYQEQEGPPIARE
                     ILTRLGAGEEVIEEVCDIIGHHHHPRAEETTNFKCLYDADTLVNLEEDLRPEPAFLQK
                     AAALVESRFLTLAGKRIAGEVLSRLGTDP"
     gene            complement(18382..20010)
                     /gene="hcp"
                     /locus_tag="PLM79_09240"
                     /gene_synonym="priS"
     CDS             complement(18382..20010)
                     /gene="hcp"
                     /locus_tag="PLM79_09240"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOV86534.1"
                     /translation="MFCYQCEQTAKGEGCTKIGVCGKQPEVAAEQDLLVYALQGLALV
                     AAEGRKVGVSDPAVNVFTVEALFSTLTNVNFDPARFVRLIHRTVELREALKSKVKAAG
                     GRADLDHPSASFEPAKDAEGLAAQGEKVGLKSDPSVDPDVLSLRHTLLFGLKGIAAYA
                     DHAHILGKEDDSVYAFVHEGLAAATRNDLGLNDMLALVLKCGETNLKTMELLDAANTE
                     AYGHPVPTPVSLGARKGKAILVSGHDLKDLEELLKQTEGKGINIYTHGEMLPTHGYPQ
                     LKKYPHMYGHYGTAWQNQQKEFANFPGAILMTTNCIQRPQGSYQDRIFTTGLVGWPGV
                     PHITGRDFAPVIRKALELPGFPEDTNGKSVMVGFARNTVLSIADKVIEAVKNKNIRHF
                     FLVAGCDGAKPGRNYYTEFVEKVPQDCVVLTLACGKFRFFDKDLGTILGIPRLLDVGQ
                     CNDAYSAIQIAVALAKAFNVGVNDLPLSLILSWYEQKAVAILLTLLHLGIRDIRLGPS
                     LPAFTSPKVLDVLVKNYNIKPISTPEQDLKEILG"
     gene            20258..20944
                     /locus_tag="PLM79_09245"
     CDS             20258..20944
                     /locus_tag="PLM79_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOV86535.1"
                     /translation="MGIVNQIGGIPLFSGLPEKQLDELAKIVVTQRHKKGKNLFSEGS
                     KAAGFYVIVSGKVKIYKLSLEGKEQILHIFGPGDFFGEVPVFAGGNYPASAETLDHTE
                     LMFFPRDAFMDLVRKEPSLAVNMLAVLSLRLRQFTHLIEDLSLKEVPGRLAAYLLYLS
                     DQENGSPSVSLDITKGQLAGLLGTIPETLSRILGKLSQMEIIRVEGRKIDLLDPEALK
                     NLAQGEKLIS"
     gene            complement(21012..21428)
                     /locus_tag="PLM79_09250"
     CDS             complement(21012..21428)
                     /locus_tag="PLM79_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOV86536.1"
                     /translation="MKSYRKELWFHVPTRRAFLNITPKVRECLGESGIREGLVLCNAM
                     HITASVFINDDEPGLHQDYEDWLERLAPHEPVSRYRHNVGEDNADAHLKRQVMGREVV
                     VAVTEGKLDLGPWEQIFYGEFDGRRKKRVLVKIIGE"
     gene            complement(21497..>21944)
                     /locus_tag="PLM79_09255"
     CDS             complement(21497..>21944)
                     /locus_tag="PLM79_09255"
                     /inference="COORDINATES: protein motif:HMM:NF021831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HOV86537.1"
                     /translation="ESYWTWATQAGYIYVTRGPLGLGAESSGAGDAVRSSQPPPPLPP
                     GAFLKLTSPSKGATLAAGRPTVIRWKSSGISPEGFDPRVDLYLSRNGGKDFTRIASKV
                     KNTGKYRWQVPRKGCNRCVLKVVSVLYPEVSDVSDRPFAIVRDAGR"
BASE COUNT         4296 a         6554 c         6509 g         4585 t
ORIGIN      
        1 gaacgcatgg aagcggttct cgaggagccc tacatccttg cctgtgaaaa gaaaatcagc
       61 aatatgaagg atctgcttcc gctcctcgaa cagatcgcca agatgggcaa gcctcttctc
      121 atcatcgcgg aagacgtgga gggagaagcc ctggccaccc tggtcgtgaa caagctgcgg
      181 ggcactctca aggtttgcgc cgtgaaagcc cccggcttcg gcgaccggcg caaggccatg
      241 ctccaggaca tcgccatcct caccggcgga caagtcatct ccgaagacct gggtatcaag
      301 ctcgaaaacg ttaccctgaa agaattcggc cgatgcaaga ccgtccgagt ggacaaggac
      361 aacaccacca tcatcgacgg cgcggggacc cgcgaagcca tcgaggcgcg tatcaagcaa
      421 attcggactc agattgagga gaccacgagc aactacgacc gcgagaagct ccaggaacgg
      481 ttggcaaaaa tcgtcggcgg cgtcgcggtc atcaaggtcg gtgcggcgac cgaggtggaa
      541 atgaaggaga agaaagcccg ggtggaggat gctctgaacg caacccgtgc cgccgtggag
      601 gaggggatcg ttcccggggg cggggtggcc cttcttcgct gcatcccagc cctagcccaa
      661 atcgacggcg acggggcgga ggctttcgga gtcaatattg tcaaacgagc cctcgaagaa
      721 cccgtgcgtc agatcgccaa caacgcagga gtcgagggtt cggtagtcgt tgagcgcctg
      781 aagggcgaaa acggtgcttt cggtttcaac gccgagacgg agcgttacga ggaccttgtg
      841 gaagcgggag tcatcgatcc gacgaaggtg gtccgttttg ccttgcagaa tgcagccagc
      901 gtggctgctc tcatgctcac caccgaggcc atggtggccg aaaagcccaa gaagaaggaa
      961 ccggcggcgc cggtgcccca cggcgggatg gaggacatgt actaacgagg caaaggaggg
     1021 acaggtcatg gactttgatc gagcaatgag ggtatttcgt ctgtactgca agcaatggaa
     1081 agaaaacccg gttcaggcgg agtcattgct ctggaccgag gaagaaagac ttacgattcg
     1141 ggagatcaaa gacttgatca aggaatgtgc gggactgttt gaggcggacg agcctttgcc
     1201 cctggccaag gccgtctgag gtttcgaagg tattcctgcc ggctacccgg tgaatggcag
     1261 ggactgcacg gaggcaaaag agagatcgcg ggacggcaac ctgtttcgtg ggggattggg
     1321 ggaacggttt cgggtgtttc cggaagccgc ggtccctcct ccggagtcgt ttccgcggtc
     1381 cgattttcca atgaaccttg tgggccgacg ggaggaggtg cctcccgtcg gccttttcat
     1441 gggggacagc tgggggtcgg acagatcgag gggcctcccc catcccggag tcccgttcat
     1501 gggctaggcg gacgcggtct cttcctccga acccggagcc ggattaaggc cccgcaatac
     1561 cttccccatc tgccgcaatg cctgacgaat gcggtgctca ttttccacca acgccaggcg
     1621 aaggtaccct tctccctctt ccccaaagcc gattccgggg gcgacggcca cgttggcctc
     1681 ttccatcagc ttgacggcga aagccatgga ccccatttgg gaatagggct cggggatctt
     1741 ggtccaaacg aacattcccg cccggggttt ttccacgggc caccccatgc ggcgcaggcc
     1801 tttgcacagg acatcccggc gactttcgta gatctttgcc tgctgatgga cggccggttc
     1861 gcaatcccgg agggcaacga tccccgcgat ttggatggcc gaaaaaatcc cgtagtcgta
     1921 gtatcccttg atcttggcca acccctggat gatttcccgg ttgccgacac agtacccgat
     1981 ccgccatccc gccatgttgt aggacttgga aaaggaaccg aactccaccc ccacgtcccg
     2041 ggctccgggg acttccagga agctgggagc cacatacccg tcaaaggtga tcttggcgta
     2101 ggcgaaatcg tggaccacca tgaagcggta tcgttttgca agcttcacca cctcttgaaa
     2161 gagctcgcgg cccgccacca cggaggtggg attgtgggga tagttgagca tgaggacctt
     2221 gggggtggga accagggcct ggcagacggt ggaaattcgt tcgagaaaga cctcttcgtt
     2281 ggccaggggg acctggatga cgttgccgcc ggcaatgatg gctgcgtaga tgtgaatggg
     2341 aaaagccggg gtgggcacca ccacggtgtc tccgggaccg atgagagcca ggcacaggtg
     2401 ggatatgcct tccttggatc cgatggtgca gatgacttcc ccctcactgt cgagggagac
     2461 cccatagtcc cgatcgtagt acttggcgat ttctcttctc aggttccgaa gtcccatcgc
     2521 tacgggatag cggtgacttt tcgggtcctg caccacttcg cacagcttcc gggtcacggc
     2581 ctccggggcc gggtcgatgg ggttgcccat ccccaggtcg atgacgtcgt ctccccgcca
     2641 ccgcttctcc attctcattt tgttgatcat tccgaagagg taggggggca actgatccat
     2701 tcggggagca aatcgtatga tggaatccgt tgtcatggat gcctcctata tggttgattc
     2761 tcgagtcggg tccgattccg gtcctttccc aaataaaaag ccccggggtc tgcggcccgg
     2821 ggcaacaaaa gcgccatggg ccgattctct cagcccatgg cgtcggtggt ttcagaagga
     2881 tggtctcttc acacgccgac cggcacccag gctgacctga atgcggcggc ggccgccaac
     2941 catggaaaca aggttatcct gctcatcttt gcccaacctt tctctcagtt gctcaaacat
     3001 gtatcgaatt cgccggaaaa tgtcaacgag caatccccgg cagcttcgac cccgcccggc
     3061 aaatccgggg ttcccggaag gtgcatccgg gaaaaagctt gacgaaccac gacggggtgc
     3121 tattctcttt tgggacgaac ccctcaaggc tccagggccc tgagataacg atcctccatg
     3181 tcgcgggttc cgttaggtga ttcccaagcc tccagatacc tgccttttgt gaccaatagg
     3241 gtgacagagt gagccgggga tgttgttgac tgccagtgcg tgcaggagga ttgactcttg
     3301 accgggagcg atccgtcggg aggcacgggg ctcgaggtgc ctttcccgaa tcggggattg
     3361 gggaaagggt gcgggggatc ggacggggat aagaccgtcc cgtttgcccg gtgcatgtcc
     3421 ggaaaggggt cggttgttgt gaggaatctg catggtgcct ttttctagaa gacttctgtg
     3481 gttttggctg ctgcctcttt ggatcggggc cgcggcggtc ttggccgtct cggcccagga
     3541 acccgcccag gaggccccgg gggaatcggc gacggccaaa cttgctttgg tccaatcctc
     3601 catgtgcgag aaggtggaaa accttgcccc ggttcgaccg gccatcgcat tttccgtgtc
     3661 ggtggggcag gtctactgct tttccgcttt cgagcccgtg ccccaaccca ccctcatcta
     3721 ccatcgctgg tatcatcgcg atgaattgag cacccaaacc cgtctgcgcc tgtatcctcc
     3781 caagtggtcc acgtacagcg tcatccagtt gcgcgaaacc gacaaagggg cctggcgggt
     3841 ggagatcacc gatcaggacg gccgggtatt cggcgtgttg cgtttcagca ttaccgactg
     3901 agcgaaggcg gcctgaaaac catgaacaag tttctgacct tcctttccac cgtgcggtgg
     3961 caggatctcg tggacatcgt catcaacagc tacatcctct ttcgggtgta cgtgctgttc
     4021 caggggacca acgcctttcg agtgctggtc ggaatcacct ccctgtggat cctccaggaa
     4081 gtggccgctt ccatggggct gatcctcacc agttgggcgg ttcggggcat caccgccgcc
     4141 gccgccatca tcatcatcgt ggtgtttcgc aacgagatcc gttccgtgct gcaggtgcgg
     4201 aacctcaagg cgttcctgtg gggttccccc cgccgggaac aacaggcgcc ggtggaagtc
     4261 atcgtcgaca gcatctacca gctggggaag aaacgaatcg gggcgttgat cgtcttcccg
     4321 gggaaggaag acctcgccga ggtggtgcac ggggggatcc aatgggacgg aacggtatcc
     4381 cgagagatgc tctacagcat tttctggccc aagaacccgg tgcacgacgg ggccatcatc
     4441 atccgggaca accgggtggc ggaggtgggg gtcctgttgc cgctttccca gagacaggat
     4501 cttcccagct actacgggac ccgacaccgg gcggcggccg ggctggcaga gcgaaccgat
     4561 gccctcatcg tggccgtttc cgaagaacgg gggcgggtga cggtggccca gggcaacttc
     4621 atcaaaccgg tggcccaacc cgaggagttg tcccatctct tgaaaaagca cctgaatttc
     4681 caggaccgga tcaacggaca cgaacacaag cgcgagacgc tgaagctcat cctggcgggg
     4741 gtcacctccc tgctggtgat gacggggatc tggtttggtt tcacccggag ccaggacacg
     4801 gtcatcgccc tcaacgtccc catcgagtat gtgaatcgcc ctcccattta cgaaatcatg
     4861 gacacttccg tggatgaaat ccggttgcag ctctacggat ccagcgctct catcaagtcc
     4921 cttcgtgccg gacaggtcca gctaaagatc gacctgagca aagccaccga agggcgcaac
     4981 gtatttccca tcacccagga gaacatcatt ctgccgcccg gcatcttcct gaacaaagtc
     5041 cagcccacct ccatcgaggt catcctggac gttcccgtta ccaaggaatt gcccgtgcag
     5101 gtggactgga cgggaaagct cccggaacac ctgatgctgg tctccgccgc ggttactccc
     5161 gagagtctga aggtgatcgg agggggaaag atcttggaga aagcgggcac gatctacaca
     5221 acgcccgtgc gcctggacca tttgaccaag agcggaaccg tcaccgcgtc catcgtgctg
     5281 acgccccctt ccctgaaact ggcctcggga tcttcggacc gggtgaccat ccattttcga
     5341 gtgcaggaac ggcccaaagc cgacagaagg tagcggcagg gacggggagg tgagagcctt
     5401 gcccggggac ggccccctca gggcgtgcag gggtcgatgt ccgaagcacc cccgtttctt
     5461 agggaggaaa tgattcggtt ggacctttct tcgatctcgg gaatcatctc aacgatggcc
     5521 cgcagggaag ccaggatctt ccgggtctgg tgcttgtggg gaatggaaag ctgggggtgg
     5581 tccaggaacc ggatggcgtc attgagggtc cgaagcttgt ccgcggtaaa atccacatag
     5641 gccaggcgag gccactcccg gcggatttcc tcgggcaggt gctgcatctt ttccagcaat
     5701 tcttcgctca ttcccagcca ggacgagtgc tgatatcgct cgaggacctc caggatctcc
     5761 tcccgatcgg cactccatgc gtcgctttcc gagagcaacc gcatccattt gggacgaatc
     5821 tggtgttcca accacttgag acccgctgca ggcatggggc tcaggtgggc cagttctttc
     5881 tgttcccgaa ccaaagtccg gcccaaccgc agcaggtctt cggctccgtt caggctgggc
     5941 aagcgggatt ccagcaaccg ggtcaaccga tcgagaagcg ctttccgggt ccctggttcc
     6001 gggagggctt tttccacctg gtcctcccat tgctcgcgca ggtacccgaa ctcggtattg
     6061 atggcggtac aaatgaatcc cagtttgacg agggtgttcc tcagatcgtg agcgagtacg
     6121 ttgcagctcc gaaggcgctg gcgggtgagg tctttctggg cttcggcaag ggtctcccgg
     6181 atgtgcaggg acagcaattc ggtccccagg gttcgggaaa gcttcttgat gagcttggtg
     6241 gaccggtccc ctcggtggat ggcatacggc ttgaaatgga tcttcaccac tgcggcaacc
     6301 acgtattcgg aatcctgcca aatgggccgc cccgcgcaat ccagcgggta taccagggtg
     6361 ccgagttggt ccccgtacaa gtagcgcttg ggaaccagca ccggttccat gtggtggaaa
     6421 tcttcgatct ccccgctgag ctgttttcgc acgctgcgaa tgtcctcgta gggatcgtcg
     6481 gcggtccagc tcttttgacg ttccacgcac catcccatgg cttccagcac gtggcccggt
     6541 cggtcccgat actcgatcca gctctttccc ggggcgttgt agtccgagac ctcgggcaac
     6601 ccccagtcga tctcgtccga ggcttcccgg cgaattccgg aggtgattcg aaccgcctgg
     6661 aggttgggca ttttcccgat gcggtaaatg gtccctttgg tggcgtcgct gaggcagaaa
     6721 agaatggtct caatcatatc cttgcaggtc ttctcaagga tatcattgct aatgatcatt
     6781 ggcttcaacc cctcactacg tggtgctccg gcaaaagccc accggagagc actttattat
     6841 cccggaatgt ccagggggtc aacagggatt tcatgggaaa tcctcccgga aggatggggc
     6901 cgggacggaa gggataaagc ggactcggaa ccgaaggtgt ccaatcgcct ccgggtgccc
     6961 gggcgaacga aagaacgccg acggccggcc gcacccgaga gggcgggaga cggtgggagg
     7021 cccgggtgca ggttcttcgg agaccttgag ggaactcctc tagcatcgcc caaagaggcg
     7081 ggtcgcggca cgcagccttt ggaccagttc gggggagaac tgctccgagg acatcctcca
     7141 ttcccagttc ccgtgagtga ctgccggaag gttcattctc ccttcttccc ccaggcccag
     7201 gatgtcctgc atgggaaaga ccgccatacg ggcaacggac atcatcccca ggcgcaccaa
     7261 ttcccaggcg acctcctcct ccccgatccc ccgccccagg taagcgaaca aggattcctt
     7321 ttccccgggc cccgcttctc tgcagaacca acctttcacc gtattgttgt cgtgggtccc
     7381 ggtgtagacg atgctgtccg gggaatgatt atggggagca tagggatttt gggccacatc
     7441 gccgccaaag gcgaagacga ggatcttcat gccgggaaag gcgcaggcac gcatggcctc
     7501 tcgaacgtcg gcggtaatca cccctaggtc ctcggcaatg atgggcaggg acgcgaaccg
     7561 ccggaaaaac cgatcgaaga gttgccgggt gggcaccttc acccacttgc cgccgatggc
     7621 ggtcttttgg gagcccggaa cttcccagta ggagacaaag ccccggaaat ggtccaaccg
     7681 aatcaggtcg aacaggcaaa gattgtgttc catgcgatcc agccaccaac gaaaatcggt
     7741 cttccggttt tctttccagg aatagacggg attgccccag agctgtcccg tttcgctgaa
     7801 atagtcgggg ggaaccccgg ccacgaactc cggccttcgc cgttcatcga gcttgaagag
     7861 acggggatga gcccacacgt ccgcgctgtc atagctgacg tagatgggga tgtcgccgat
     7921 gatctggatg caccgtttgt tgcagtattc cttgaggctc ctccattgcg tgaaaaacag
     7981 aaactgaaca aactggtgct cgaggacggc gtcgctcagc cgcagcctcg cgtcccggag
     8041 gggggcctcg tgacggtccc ggatctcctc gggccagtcc ccccaggccg tttcccggaa
     8101 ttcctccttg agggctcgga ataaggagta gtcttcgagc caatggccgt tgcgacggcg
     8161 gaactcgagg aaggtcgggt catcttccag gcgatccctg tgccggtcga aggctttgcg
     8221 gagcagtcgc tccttgtact gcgccgccct cgggtagtcc gtgcgataaa cgttcccggc
     8281 cggcgggtct tccaggtcgg acggatccag gattccgtcc ctcaccaggt cgtcggggct
     8341 gatgagcaga ggatttcccg caaaggccga aggactcgaa tagggagaat tcccgtagat
     8401 ccccagggtt gggttcagag gcaggacctg ccagaggctc tggccggcct cggccagcag
     8461 gtcggcaaac cgacgggctt ccgggcccaa gtccccaacc ccgtaaggag aagggaggga
     8521 ggtgatatgg agaaggagtc cgcttcgtct gtttttcatt tcttcacctc atcctctcgt
     8581 caaggaagga ggaaagctcc ctcgtccccg gcaggagagg gatggggtga gccggatgcc
     8641 cggtttcccg ggtgggagcc tcgctctcgc gggggcctgt tccggcgctg ttgccctacg
     8701 gttccgtgct cctgaggaaa acgaccccca ggggagggac gaccagagaa acggaatagg
     8761 gtctcccatg ggaggccgta ggctgggcat gaaccccgcc cgagtttccc atcccgctgc
     8821 ccccgtagat ttcggcatcg ctgttgagga cctcctccca gaagccgccc gtgggcactc
     8881 ccagccggta gtcgcttcgg ggcaccggcg tgaaattggc cgccacaaga accacccgtt
     8941 tcgtggtctt ccccttcctc aggaaggcaa tcatgctggc atcggcatcg tgaaagtcca
     9001 cccattcgaa tccctggctg ctgaaatcgt cttcgaagag cgccctttca gtccggtaga
     9061 accgattgag gtcccgcacc caactctgga ccccctggtg aaacgggtat tccagcaggt
     9121 cccagtcgag gctcttctcg tgataccatt cgtcccattg gccgaattcc cctcccataa
     9181 aaaggagctt cttccccggc tgacccagca tgtaaccgaa cagcaggcgc aggttggcga
     9241 acttttgcca gtcgtctcca ggcatgcggc gaagcagcga acccttgccg tacaccactt
     9301 cgtcatggga caggggaaga atgaaattct cgaagaaggc gtaccaaatg ttgaaggtga
     9361 gctggttctg gtggtacttg cgatggatgg gatccttgga gaagtattcc agggtatcgt
     9421 gcatccaccc catgttccat ttcatcccaa aacccaatcc acccaggtag gtcggcctcg
     9481 agaccatggg ccacgctgtg gattcctccg caatggtctg cacatcgggc tgcgtttcgt
     9541 aaacggcctg gttgaaacgc tggatgaaat agatggcgtc gaggttttcc ctccccccgt
     9601 atggattagg aatccattct ccttcctttc gggcataatc caggtacagc atggaggcca
     9661 cggcatccac ccgcaatccg tccgcgtgat agcgattcag ccagtggatg gcgctgctga
     9721 tgagaaagga gcggacctcg tagcgtccgt gattgaaaat gtagctgttc cactccggat
     9781 ggaacccttt tttggggtct gcatgttcga agagataggt cccgtcaaaa tacacgagac
     9841 catgctcgtc gctgggaaaa tgggacggca cccagtccag gatcaccccg atgtcgttgc
     9901 ggtgcagccg gtcgatgagg gccatgaagt cctggggagt gccgtaccgg gcggtgggag
     9961 caaagtaccc cacggtctgg tagccccagg aaccgtagaa ggggtgttcc atcacgggga
    10021 ggaattccac gtgggtgaaa cccatgtccc ggacgtagct caccaagtaa tcccccatct
    10081 cccggtaggt gagaggccgg ttgtcttcct cggggacccg tcgccaggaa cccaggtgga
    10141 cctcatagat ggaatggggg gcattcagag cgttggcttc ccggcgtcga ctcatccaat
    10201 ggctgtcttc ccacgggtaa tcgaggtccc acacgatgga ggcggtcttg gggggggtct
    10261 cccagaaaaa ggcgaagggg tcccccttgt ccacccggta cccgttgctc ttggagaaga
    10321 tatggtactt gtaggtcgcc ccctgctcca ggcccggaat aaacccttcc caaatgcccg
    10381 atccgtcccc tctcacgtcc aggggatgag attcggggtc ccactggttg aaatccccga
    10441 caacggacac ccgttccgca ttgggggccc agacggcaaa gtggacgcct cgaacacctt
    10501 ccaccgtaag aatgtgagcc cccatttttt cgtggagccg gaaatgattt ccctgcttga
    10561 acaggtaaat gtcatggtcc gtcagcatac ttccccggta ccaaacttcc tgagtcatct
    10621 tgatgtcctt tctccacccc gggattcctt tcggcggtgc catggcttcc ggtgccgggc
    10681 aggatgtttc cgagccccat cctgcagtcc cccgtgcgca cggagcagcc gggaaacgcc
    10741 ctggggcgct gtaaggccgg gagctcagcc cttaccgatc agtcgcaatg gggaatatgc
    10801 ccggaaccga agaaaagggc aaggaacttt ccccgcccga gtttggagag gaagggcttt
    10861 gcgaacgggg ccctttcccg ggcggaaaga aaaggacccc gcctcggaag aggcggggat
    10921 tggattcacc aacgggggtc cgactacggc tcgtcggaac ctaacccggg agggccgtga
    10981 tgatggaggg ggttttcacc atctcgtgga gttggcgctt ggccactttg tatcgttcga
    11041 agtacacttg agtgatcttc ttcatgagct tgtagcccaa ctcgggatcc gcggcgcaca
    11101 tctcgaggag cttgtcggca tcgacggcca gcacatcgga atcttccagg caaaccccgg
    11161 tcagggtata gaagcgctgg tccgtaaaac aggccgtccc gaaaagctca cccctctggc
    11221 gcatctcgaa cgcaataccg atctggtcct cgggcttgat ttcccgcagg ctcacaagtc
    11281 ctttctccac gacgaacaaa taccgggcgg gctccccgct ctcatagacg cgtccgttcg
    11341 ctttgtaaga tttcctttgg gtgacccctg caatcttttc caactgggcg tcggaaaagg
    11401 cttcaaacaa atcgaatttc ctcagttcac taacctctac catggcgcgc ctcctttgtt
    11461 ggtcgttccc gccttcttcc ttcccgtcaa accatgcccc ggagagtcac accgaacgag
    11521 ggcccctccg ggagggccct gattcctcga tcttctgcaa ccagcggccc aggatgttgc
    11581 tgggcgtgtc gccggtcaac aagcagggtg aggtcccgta ataaaggttg ttgcatatgg
    11641 aggaccggcc caccagcttg ggatgagtca ccagaacata aagcaggatt gccggggtga
    11701 actccttcaa tccataggtg caaaggatcg agcacttctt gcccttgaga aaaccgttga
    11761 gctcggtctc atattcgatg agacattccg atccccggac tccgcccagg atccaaccca
    11821 tatcggcgat gaggaaaaga ttcggatacc cccgggacag ggcttcatcg atccggccgc
    11881 ccagcctatc gaggaccgca tgagggctga aagacccggc cgtcagaaaa aactcctccg
    11941 ccttttcgaa atccaactgc ctgctgaaca ggttcgggtc gatgtcccat cccgtccccc
    12001 tcaggtagtc gatcagacgg ctgcggcccc gtttgtgcaa gacacagagg acctgatctc
    12061 ccgcaaggag ggaagtcgtc aggaccgggg cgatggaaga acacaggtcc gggaaagaat
    12121 caaaaaagag gcatagatga tccggggtaa ggctccggtt ttcggcattg tgcaaagtat
    12181 acacggcaat cccctccgca ttgcccgcct gactgtaatg tgcacggtgt tgtgccgttt
    12241 gtccatggag cgaccggttc atttccatgg ggaagattcc cggtccctgg ggataagccc
    12301 cataagcacg gggacccgga ccgtcgcgcg ggtccgtgtg ccccggatct gtatcgggcc
    12361 cgggggcaga ggggctcgat gaggaaggcg gggcggggtt cattccttcc agcggtcgat
    12421 gtcaatgagg cccaatcggg ccgcgtatcg aatcaactcg agggtgctgt gaaggtccaa
    12481 tttctccatg attcttgccc ggtggttgtc cacggtcttc gagctgatgc acaaattggc
    12541 ggcgatctcc tttttggaga gtccttcggc aatgaggcgc aggacctgct gttcccgggg
    12601 ggtcagggtc ccgtatttcc tgtccgtgac ctttgcttcc tttcgaggca aatccaccag
    12661 acgttccacg acccggctgg acagggagca gtcgagatag aactcccctc gcgatacggc
    12721 ttccaggcct gcaaccaggc ggtccgccgc cgattccttc accacgtagc ccgtggcccc
    12781 ggcctgaaag gattcggtga cgtaatcgat tttggcgtgc atgctcaaca tcaggacccg
    12841 ggtctgagga aggaccgcga gaatgttccg agtgagctcg atcccgctct tgtcgggaag
    12901 ggaaatatcc accaccacca aatcgggtct taattccccg gccagccgaa gtccttcggc
    12961 ggcgtctccc gcttctccgc tcacctggaa agacggattt cgtgaaatca gagacttgat
    13021 tccttcccgg aagaggggat ggtcgtccac caccaggatg gttgtcttcc tagccaacac
    13081 ccttctcctg caaggggaac tcgatacgga ttcttgtgcc gtggttgggc cgggaatcga
    13141 tcttcatcct accccgcagc agcgcgactc gctcctccat gcttcgaatc cccatgcacc
    13201 gttcgttcag ggaagaccat agtcggctct ccacgtcaaa ccccgtgccg ttgtcttcga
    13261 ttcggagaat gatcttgggg taggaggcca ccaaccggat ggcgacccta gtcgccttcg
    13321 aatgtttctt cacgttgctc aacccctcct ggatcaaccg aaagagggtg atttccgttt
    13381 cagaccccag atggaggtca tcgatcccgc cggccacaaa gtccacctcg atcccgtttc
    13441 tccgggcgaa cccttcgcag gttcgatgga tggttctcac cagtcccagt tggtccaagg
    13501 tgggaggacg cagatgatag gcaaggcccc tgagggcctc gatgctctcc tggagcattt
    13561 ttgaaagctc cgcgactcga ttccgcaatg aagggtgcgc ctcttccgtt ccgtccagca
    13621 gggtatccag tccgatcttc agggtcgaca ggtcctgcat gatgacgtca tgaagatcgg
    13681 cggagagcct ctgtcgttcc atctcctggg cggacatcaa ttgccgggtg agataccgaa
    13741 tgtgttcctc ggccctcttc cgaagagtga tgtcccggtc gatgccgcgg tatcccacca
    13801 gggcgccgga cgggtcccga atgggcaccg cgctggtctc caccatgatt tcgtgcccgt
    13861 ccttgtgaac cagtcgtttc acgtatccgg agatgggacg cccttgatcg cccaacggca
    13921 gggcctcttt cttctgactt tcccgctcct gcggcgcaga gaagtcgtag aagtgcttgc
    13981 ccaggacctc ttcggccgta aaccccagga tggcttccac aaggggattg gaataggtgt
    14041 accttccgtg ggtgtcgact tcccaaatcc agtcggaggt tctttctgcc aagtcccgga
    14101 aacgtcgttc cgtctctttc agggattccc ggagtctctt ttcccgaacc accttttcca
    14161 ggagcctggg aagcagggcg aagcaatcgg cggacttgac caggtagtcc cacgccccca
    14221 gcttcattgc ctgagcggcg atcttttcat cttcccgccc cgtaatcatg atgacgggaa
    14281 tgttgtgccg tctctcgttg aggaactcga gaaactgaat tccgttcatg ccgggcatgc
    14341 gatcatcggt cacgatgagg tccggctgga tccgatcgat ttgctccagg cattcggaac
    14401 agtcacggaa atggtgaagg gtcgcatggg gaaggtcctt gttgatcacc cgtttcatga
    14461 gtcggaagtg ctcttcctca tcttcgatga cgaccaggct cagagcttcc gcttcgccga
    14521 tatccgaggg gttgtctttc cggatcattg ggcgccattt ccgtttcaag agatcgtccc
    14581 cgggcatttc cgaccgcggg ttgagccgtg ctccgggttc tggcggtcga atctttcacg
    14641 gcgttcttgc cccggcaaaa gggcgctgcc caaaggcccc cgctcccggc aatgctgttg
    14701 agtgagtgaa acggcactta ggaattcccc ctcgaagggg acagggggtg taggtgttgt
    14761 gatcgcacct catccctccc aaagggggaa atcaacccgt ttcgttcaac ggcattgccc
    14821 cagtcgcggt gtccatccgg gagaggctcc gtgccgtcgg gtcctttgcg gttggaactc
    14881 ttcctgaatc cgtgctcgtc tcgaggcgcg tgcttgggcg gatgagcggg ggatggtcaa
    14941 ggagaaacac acggttcgct ggctgcgggt ttgccgacgg aattttcggt agcgccccca
    15001 aaggcaccga tgccgctttg aaaagtaccg taatttttac cagaactggg aagttagtca
    15061 aatcctatcc caaccccggg atcggggagg ccgggttgcg gcaccgccgg gagccgctat
    15121 tgacaggaca tccggactcc cttattgttc cgcggtatat cccaagatcc ggaacccgtt
    15181 gggaaaatcc tgcacctgcg gaaagggaac cgggttcata aaccgtgggg caagctctta
    15241 gaggagaacc tccttggaag tgatctggca aggaatggtg gaagccggac ggctattggc
    15301 gaccctggat gccgatgtca tccacatcac cctccgaacc ctccaggtct ccgtcacggc
    15361 cacgttgatc agcatcctca tcggcattcc cgcgggaacg gccctggccc tcaccaattt
    15421 ctggggccgg aactttgtgg tcagcgtggt caacttcggg atggggcttc cccccgtggt
    15481 ggtgggactg atcacctggc tgctcctggc ccgttacgga cccctgggat ccttgagcct
    15541 gctctacact cccacggcca tcgtgatcgc ccaggccatc atcgcatccc ccattgtcgc
    15601 cgggttcagc ctggccgctc tccagtcggt caatcccaaa cttcgattgc aaatcctctc
    15661 cctgggagcc aaccggtttc agtacttcct cctcctcctc tgggaagctc ggttggggat
    15721 tctggccgca atcatcgccg ggtttggagg ggtcatcagc gaagtcgggg cctccatgat
    15781 ggtcggggga aacatacggg gctacactcg agtcctgacc accgccacgg tgctggaagt
    15841 ctccaaggga aaattcgagc tggcaacggc cctgagcgtg attctcctgg tcctctcgtt
    15901 ttttgtgacc gcttctctga gttacctcca acagaggggc agaagagcgt gaatcccgtc
    15961 attgcagccc acaatctcaa ggtggttcgc ggggggaaac tgatcctccg ggtggacgag
    16021 ctggccgtgg agaagggaca ggtgctggcg gtggtcggac ccaacggggc cgggaaaacc
    16081 tgcctgctcc ataccctggc actcctggaa cccccggcct ccggaaccgt ccggctcttc
    16141 gacgtggacg ctttcaacgg caatcagctg gccctgcgcc gccgaatggc cctggtattc
    16201 caggaaccct tgctcctgga caccaccgtc aagagaaaca tcacccttcc cttacgcatg
    16261 agggggatct cgggacggga agccaacgct cggtccgatc gatggctcca tcgtttcggg
    16321 atcggttccc tttccgctcg ctccgcccgg gctctttcag gaggagaagc ccagcgaacc
    16381 agtcttgctc gggccttcgc catggaaccc gagatccttc tcctggacga gcctttcacc
    16441 gccctggatt acccaacgag aaagagcctg gtggacgaac tgggagaagt cctcaaggag
    16501 atgaacctca ccacggtctt tgtgacccat gattactcgg agatccctcc cctgaccgaa
    16561 caggtggcgg tgttgttcga tggaaggatc gtcaaacgcg gttcttcccg ggagattttc
    16621 ggggaggatt ttctgaaaaa gaagtcctac gccccgtggg aaacctaacc gcggcgagtc
    16681 gcccgggtgt gggacgctgg tttttcccaa cccggaccta acccgccgag agattttttc
    16741 gcaggatctc gccccccact ccgaggacga tctcctcgag ggggactttc ttgcccgctg
    16801 cgtccattcc cctcttcacg atcaccggca atgccgagca gcaggggact tccatgtcca
    16861 ggaccgtcac gctttgaatg tcggcctctc ggaaaatttc ctcgaatctt ttgatgtatt
    16921 ccggtgcatg gtcaaatttc ggacatccca gcagcaccac ccgatccttt agaaagtccc
    16981 ggtggaagtc cggataggcc accggcgtgc agtctgcggc aaccaggagg tgggcccctt
    17041 tgagaaaagg agccttggcc ggcacgagcc gaatttgaac gggccagtga gtgagggcgg
    17101 acggaatcct ttgggacccc gatgagagat ccgcaccggc aggcttgggg gccatgaccc
    17161 gaatcatggt cgatgggcat ccgcacgccg gggcgggatg ctgcgcctcc cgggccttct
    17221 ttctttcctc gatgtactgt tccacggcgg cgggatcgaa ttcgcccgcc tccctctcga
    17281 tgatccggag ggcgccctga gggcattccc ccaggcaggc tcccaatccg tcgcagtaga
    17341 cgtccgaaac cagttttgct tttccgttga cgatctcgat cgccccttcg gcacaggcaa
    17401 cggcacactg accgcagccg tcgcaccgct cctcgttgat ctcgatgatt ttccttgtcg
    17461 agctcataac ggttctccag gagttccagc ggttattctt cggggggatt ggtttgtcgc
    17521 ccccccctcg tccggcaatc caccctccat gggagcggga cggggtcacg ggtccgtccc
    17581 cagccgggag agtacctccc cggcgatcct tttgccggcg agggtaagaa accggctctc
    17641 gacgagggcg gccgctttct gcaggaaagc gggctctggg cgaagatctt cctcaagatt
    17701 gacgagggta tccgcatcgt agaggcactt gaaattggtg gtttcctccg cccggggatg
    17761 gtgatgatga ccgatgatgt cgcaaacctc ctcgatcact tcctccccgg cccccagtcg
    17821 ggtgaggatt tcccgggcga tgggggggcc ctcctgttcc tggtaacggg ctccggtgct
    17881 gttgtacttt cgctccgcct ccttgatgcc gatgtcgtgc aggtatgcgg ccatgagcac
    17941 caccgcaggg tctccttttt cttccttcac gattcgctcg gcataccggg ccactttgct
    18001 tgcatgcccg atgcgccgaa aatcctgttt gaagtacctc ttcatttcca aggccacgcg
    18061 gtctttgagg agctcctgcc gttgggccgt gagttcgggg gggagctctc ccaggcattg
    18121 ggccgcaaac ctgcaatagg aggcacaccc gaaatccatc ttgggattcg tgaccgtgtg
    18181 accgcagtgc ctgcactttc tccgggtctc atcccgaaag aactcgacct cgtgaccgca
    18241 ttcggggcac ttgacctcga agatggcgtt ctcatcccaa aatcggctgt cttgcccggg
    18301 acacttcatg gctctcatcc cccgcgagtt gcgttgtctt tttcaaaggg gagacgggct
    18361 tttcggcccg cctccgcata tttagcccag gatctccttg aggtcctgct ccggagtgct
    18421 gatgggcttg atgttgtagt tcttgaccag aacgtccagg actttgggac tggtgaacgc
    18481 cgggagggag ggacccagcc ggatgtcccg gattcccagg tgcaacaagg tcagcagaat
    18541 ggcgacggcc ttctgttcgt accaggagag gatcagggag aggggaagat cgttcacccc
    18601 cacgttgaat gccttggcca gggccacggc aatctggatg gcggagtagg cgtcgttgca
    18661 ctgccccaca tccaggagcc ggggaatgcc caggatggta cccaggtctt tgtcgaagaa
    18721 ccggaacttt ccgcatgcca gggtcaggac cacacaatcc tgggggacct tttccacgaa
    18781 ttccgtgtaa taattgcggc ccggtttggc cccgtcacag ccggcaacca ggaagaaatg
    18841 ccggatgttc ttgttcttca cggcttcaat caccttgtcc gcaatgctca ggacggtatt
    18901 gcgggcaaac cccaccatca cggacttccc gttggtgtct tcgggaaacc cgggaagctc
    18961 gagggccttc cggatgaccg gggcaaaatc tcggcccgtg atgtggggta cccccggcca
    19021 gcccaccagc cccgtggtga agatccggtc ctggtaggat ccttggggac gctggatgca
    19081 gttggtcgtc atgagaatgg cgccggggaa gttggcaaat tccttctgct ggttctgcca
    19141 ggcggttcca tagtggccgt acatgtgggg gtatttcttc agttgcggat acccgtgggt
    19201 cggaagcatt tccccgtggg tgtaaatgtt gatccctttt ccctcggtct gcttgagcag
    19261 ttcctcgagg tccttgaggt cgtggccgga aaccaggatg gcctttcctt tcctggcccc
    19321 cagggacacc ggtgtgggaa cgggatggcc gtaagcctcc gtattggcgg catccagcag
    19381 ctccatggtt ttcaggttgg tttccccgca cttgagaacc agggccagca tatcgttgag
    19441 tcccaggtcg tttctcgttg cggccgccag cccttcgtga acaaatgcgt agacggaatc
    19501 gtcctctttt cccaggatgt gggcgtggtc ggcgtaagcg gcgattcctt tgaggccgaa
    19561 aaggagagta tggcgaaggg agaggacgtc cgggtcgacg gaggggtccg acttgagacc
    19621 caccttttcc ccctgggctg ccagcccttc cgcatccttt gcgggctcaa agctcgccga
    19681 cggatgatcg aggtccgctc tgcccccggc cgccttgacc ttggatttca gcgcctctct
    19741 caattccacg gttcgatgga tgagacggac aaagcgcgcc ggatcaaaat tcacattggt
    19801 caaggtggaa aagagggctt ccacggtaaa cacattgacc gccgggtcgc tgaccccgac
    19861 ttttctcccc tccgccgcaa ccaatgccaa tccttgcagc gcatacacca gcaggtcctg
    19921 ctccgccgcg acttccggtt gctttccgca gactccgatc ttcgtgcagc cttctccctt
    19981 tgccgtttgt tcgcactggt agcaaaacat cttcttcctc cttttcgaag ttattgattt
    20041 accatcctaa tccctcgcag ctttcgggga tgcccggatt tccatggtgg aaggacatgc
    20101 ggacgtcata acccgggcac acgggcccgg cgcttttgga gatttccatt cctccggagc
    20161 acctccgtcc gttagggaga actataagac ttgatttgcc tcggtgcttt gacgtaagtc
    20221 aaagaacaac gatttcccgg gagagggggc gaacggtatg ggaattgtga accaaatcgg
    20281 cgggattccg cttttttccg ggcttccgga aaagcaactg gatgagctgg caaaaatcgt
    20341 ggtgacccag aggcacaaga aaggaaagaa cctcttttcc gagggcagca aagctgcggg
    20401 cttctatgtc attgtctccg gcaaagtgaa gatctacaaa ctttccctcg agggaaagga
    20461 gcagatcctt catatcttcg gacctgggga tttcttcggc gaggtgccgg tctttgcggg
    20521 cggcaattat ccggccagtg ccgaaaccct cgaccacacc gaactcatgt ttttcccgcg
    20581 ggatgccttc atggatctcg tgagaaagga gccctccctg gccgtcaaca tgcttgccgt
    20641 cctgtccctt cgcctccggc agttcactca cctcatcgag gacctgtccc tcaaggaagt
    20701 ccccgggcgc ctggctgctt accttctcta cctcagcgac caggaaaacg gctccccgtc
    20761 cgtctccctg gacatcacca aggggcaact ggcaggcctt ctcgggacca ttcccgagac
    20821 tttgtctcgt atcctgggca agctgagtca gatggagatc attcgggtag aaggtagaaa
    20881 gatcgacttg ctcgacccgg aggctttgaa aaacctggcc cagggggaga aactgatctc
    20941 ctgaaaggct gtaggaaagg ccccgctccc accccctccg gcgggcttcc ttgggcagcg
    21001 agaccgggag gtcattcccc gatgattttt accaggaccc gtttcttgcg ccgcccgtcg
    21061 aactccccgt agaagatctg ttcccagggc cccaggtcca gtttcccttc cgtgaccgcc
    21121 accaccactt cccgccccat gacttgcctt ttgagatggg catcggcgtt gtcttctccc
    21181 acattatgcc ggtaccggga cacgggttcg tggggagcca gccgttccag ccagtcctcg
    21241 tagtcctggt ggagcccagg ttcgtcgtcg ttgatgaaga ccgaagccgt gatgtgcatg
    21301 gcattgcaca acaccaagcc ttcccggatc ccgctctccc ccaggcactc tcgaaccttc
    21361 ggggtgatgt tgagaaaggc ccgccgtgtg ggcacgtgaa accacagttc cttgcgatag
    21421 cttttcaagg tcgtccaatc ctccaggacc ggtttgggct cgattcgcgc ttcccggcgc
    21481 aggccggccc gtcttcctat ctgccggcgt cacgcacaat ggcaaagggg cggtcggaaa
    21541 cgtcggacac ttcgggataa agcacggata ccaccttcag gacacaccgg ttgcagccct
    21601 tgcggggaac ctgccaccgg tacttgccgg tgtttttcac cttggaggcg atccgggtga
    21661 aatccttccc cccattgcgg gaaagataca gatccacccg aggatcaaat ccctccgggg
    21721 agatccccga ggacttccag cggatgacgg tggggcggcc cgccgccagc gtcgcccctt
    21781 tcgacggtga ggtcagtttc aggaaagctc ccgggggaag aggaggggga ggctggctcg
    21841 aacggacggc gtccccggca cccgaggatt cggcgcccag accgagagga cctcttgtga
    21901 cgtagatgta tccggcttgg gtcgcccacg tccagtagga ttct
//
LOCUS       DAOOVC010000058        20653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1748_length_20653_cov_6.082047, whole genome shotgun sequence.
ACCESSION   DAOOVC010000058 DAOOVC010000000
VERSION     DAOOVC010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 20653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20653
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_20653_cov_6.082047"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..872
                     /gene="radC"
                     /locus_tag="PLM79_09260"
     CDS             195..872
                     /gene="radC"
                     /locus_tag="PLM79_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOV86538.1"
                     /translation="MDSDNQGHRRRLRERFLDSGLDGFHDHEVLEFLLSFAIPRKDLK
                     PIAKGLLEKYKTLSAVLDASSERLEKEDGMGPYSAVFLSLMPRLVERYQQDRWKAKPV
                     LCSTRDAVAYLTSLLGTERNEVFYVLALDSKNSVLAGEGVQRGSVNRTAVFPRQVVEV
                     VIRHRATAVILAHNHPGGDPTPSASDRQLTRKLKRILNDLDIHVHDHIIIAGPKYFSF
                     AEKGELE"
     gene            869..1894
                     /locus_tag="PLM79_09265"
     CDS             869..1894
                     /locus_tag="PLM79_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HOV86539.1"
                     /translation="MTGEMEAVLVKETGAKGDWNVLEIGRIALPALRSEEVLVRVEAC
                     SVNRADLLQRRGLYPPPPGASSVLGLDFAGIVWETGTKATAWKRGDRVFGITAGGGYA
                     RYAAVHRDHLVPIPENLGFVEAAAAAEVFFTAHLNVFLEAGLKQGETLLLHGGGSGVG
                     TAATQLASLAAARVLVTAGSRDRVQRCLDLGASFVINYKEEDFAARAMELTRGEGVDV
                     ILDWIGAPYLQKHVRILKPRGRLVLIGLMGGNTGDIHLAPLLTKRLRIIGSVLRSQSV
                     REKAALTASFKEKVLPLLASGAVRPVLHRVYPMEAVEEAHQLMKSGAHFGKLVLSWEK
                     PGEAQGP"
     gene            2067..2945
                     /locus_tag="PLM79_09270"
     CDS             2067..2945
                     /locus_tag="PLM79_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86540.1"
                     /translation="MENEWISLEQVTFVRNRRTILDGIDWRVQPGDHWVVLGANGSGK
                     TTLLQLLAGYLWPTQGSVVVLGERFGHTDLRELRKRIGWVGSFLEMQVPSTTKPLDLI
                     VSGKYASIGIFEVPTQEDYREAQDLASRLGCERILDSPYGVLSQGEKQRLLIARALIH
                     RPPLLILDEPCAGLDLAAREQLLRTLDDMGRGNPGLTMILVTHHLEEITPVFSGVLLL
                     KEGRCTAQGQKEEILSPDRLNHVFGIPIEVHRKQGRYWAAMRFEDDAQDLFGNKTPPS
                     PTNLGGGRRTGIRSER"
     gene            complement(2929..4266)
                     /locus_tag="PLM79_09275"
     CDS             complement(2929..4266)
                     /locus_tag="PLM79_09275"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOV86541.1"
                     /translation="MSLFPGKKKPRPLWTLFPAAVFFLSTVLVLGATGWAQSLQSAEI
                     ASSPNPVGSGARALGMGGAFIGVADDATAASWNPGGLTQLECPEISAVGTAVLRSEDN
                     LFPDHSEASEKQTVSRGGLNYLSAACPLVVADTNVVASLNYQHLYDFTREWNTRLHSE
                     GLKQAWKVDQGGGLYALGIALGVQVSPRLAVGCTLNLWEDLLYRNGWEQVIRTRNRVR
                     DILGDLHRVNHYVRNRYDFSGWNVNVGALWNLTEHLSLGLVFKSPFTAELAHRTTSVI
                     RYLPYPAYRQVNFLDEELDMPMSYGVGLGYRFSDKLTAALDVSRTQWEDFVYRSARGR
                     EFSPVSGLPLGKSRIEATTQVRAGFERLFIRPGFVVPLRAGGFYDPAPAEGGHDPYYG
                     FSLGSGIGVGRTVWDIAYQVRLGTDVGSSIVRPQRFSQDVCEHTLFTSLIVHF"
     gene            complement(4286..4543)
                     /locus_tag="PLM79_09280"
     CDS             complement(4286..4543)
                     /locus_tag="PLM79_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOV86542.1"
                     /translation="MPSSTGFVTGIVTDALSGAPLEGVTLTIDDEAVSVSSGPRGQYL
                     LAGAAGNHTLVARKSGYKRYAGKITLLSGVTKTRNVSMAPA"
     gene            complement(4639..6660)
                     /locus_tag="PLM79_09285"
     CDS             complement(4639..6660)
                     /locus_tag="PLM79_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOV86543.1"
                     /translation="MAGIFSAVLSVLLLLGLWACETGDLIKIGAPPERTGGLMKGTFR
                     GRWWHYYHRGISRAEAGAWQEAEADLKHALKLRGKDQRRARTYGMHFTDYFPHRELGI
                     VYFRRGRFEEATREIEESLGSEKSAKAEYYLDLVRKEKIREEKTPASVPEILLSSPDP
                     DTWVRTPVARVEGLVRDPGFVKRVTVNGEPVRLDLAAREVPFHRQVKLRGGSNPVVVE
                     AANLEGRISRLERWVVLDSQGPTIGIEGVFRESGRKSPGFRIRGIVHDDAGLEEVVVN
                     GRVVPIPSGKELRLDLPVFPAPGSDRVLVEARDRVGNATRAEIPLESLSRERRTLTAS
                     RTPVSPSPDEGDQRGGDVFPPLIELRNWSAQQQVFLDQVYLEACVRDESGVAEVRLNG
                     RPLPRSAGKRVHLAHLASLEEGANDFVIEARDLHGNKAEKTVRFERRLQKVRDIGSRL
                     PVALLPLDGRPDGGGSAADALEELILGDLVRQGRFALVERRRLEEILREQRLGAGGLA
                     DADTAVRIGKILAAGGMLMGTVREKGPTLEIYLRLVDTETSLVIAAVDVYGEERDLPS
                     LHELSRGLVLKLCDEVPLLEGTVVRVQDNRLTVDLGKESRLKRGMRVIVFREGEPVRH
                     PLSGVFLGADMEVLGWGLVRAVGEQMSEVEAPAGNGPRSFEPLQKVLTQ"
     gene            complement(6663..9869)
                     /locus_tag="PLM79_09290"
     CDS             complement(6663..9869)
                     /locus_tag="PLM79_09290"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOV86544.1"
                     /translation="MTLHDLPKDDDPGSPVSSIEEFLRRREDLDRSIRDRFQRPVAVA
                     FTDIVASTDYFEKLGDLEGRLMLQRHHDLLRPVIEAHGGTVTKTLGDGLMVSFRDPVR
                     AALAAAEIQRRLASENKGREPLEQIRVRIGMHWGLAIVEETDIHGDVPNTAARLCSLA
                     KKGDILVSRSLVDAVRDAPEIAHEYVGAKTLKGKTLPVDVYRILWDPFQEADLKRTLP
                     PGREGPESGGASCRLTFQMEDDILRIDASWGKREDGVPRSLRVPFQEREVRELVEEID
                     ECLLGTDSRGRVSKEQVRRLENLGTNLYEILVPRDLAKEVLDRRPDSVLCGIDPALVH
                     IPWELLHDGESFFCLKYSMGRMVLSSPSTDLPRGKSRPAGVGVLIVADPRGDLTAARA
                     EGISVRRVFTRKDIPGLRVYLSSREATCAFLEDQLPQYDLFHYAGHWDFDGADPSLSG
                     FLLSDGKFETGRLLGLARRKRLPRLVFANACRSVRDPGGSSGERLHGLAGGFLQSGVR
                     HYIGSRLDVFDRSSSAFAREFYTRLLEGSPVGEALRYARQRSIEHYGKETLTWASYVL
                     HGDPLETFPDRGVAPAGAFSFLSRFWRNKVRRWPVLSLVSALVLILGLFVLGIRRESP
                     GPNALRRPGEEAFHLLHTGQISRAEGLLQSMTDKNAVYYQGLSALALAKGNVEEAREA
                     WKSARSREPDDPYLDVLAARFALASGNTDEARLSFRKIANEEKLQPWQRAEIHLDLGR
                     IELMRGSLNEALEEFDRALQADPAFLPVYTARGLTLERLGRLEGAVDSYEKGASVNCD
                     DVVNFGLYRRSRQEKEARRNEEQKKRIDDLVAQLVQAYGEGPRSLSGADPWTSRPLSL
                     FFLDLEPRGAPSPREGEDAFFSQLIAGELAECPRIQVVERALLDRLLEELRLSSSQLA
                     EPQTALRLGKILSARVLCTGSILRHQGRVQLNLKAVDTETTRVMAHSSALVPGGEDPV
                     AAVRKTSRQFRERLTEAYPVRCRIREVLGSQLTLNAGSLCGIATDMRLRVMEPEGKGA
                     ELVVLDVSDGSSTAVLVSPQARLQPGWRVEEIPGARGG"
     gene            complement(10412..11281)
                     /locus_tag="PLM79_09295"
     CDS             complement(10412..11281)
                     /locus_tag="PLM79_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOV86545.1"
                     /translation="MNPTRSPQSPRPARRFSGDSAAAAVVFAAGKGTRMVGYQGNKTL
                     LPLIPGGSPYNGERPLLLEVLSNLPPGPKGIVVHHCARQVKEATAGQGAVYLFQPSTN
                     GTGGALLSARPFLEKVREDSVLITMGDVPLIRSRTYGELLTRLDRHEMMILAFEPRDR
                     AQYGMLEMEEDRVTGIVEWKYWHAFPPDRQAALRYCNAGVYAVRRPVLLDYMSILATR
                     PHRVQKQRGTEWVTIEEFFLTDLVEWMSRDGLSVGWVTAPEEEVCGVDTPESLRFVQE
                     QYARRKSGDSGRC"
     gene            complement(11278..12432)
                     /gene="mltG"
                     /locus_tag="PLM79_09300"
     CDS             complement(11278..12432)
                     /gene="mltG"
                     /locus_tag="PLM79_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOV86546.1"
                     /translation="MGRGRRYVLWGCFCLVSLIGLSAVAAGLVCFRFWLFLRLPPSVQ
                     PGVRWVEVRPGMGAMGIARLLRENGVVADAVQFRVLCWIRKGDQKLKAGEYAFSTLLT
                     PEQVLDHLVHGRVFLHRLTIPEGSTLRDTARIIGEKHLASPDEVLRLSADPDFVRSLG
                     LDSPSLEGYLYPDTYLLPRSSTASSILKAMVQQFRTRFPEEWKDRVSQSGLSVREVVI
                     LASMVEKEAVVDAERPVIAAVFFNRLKLGMPLQSDPTAVYDLDGFTGPITSAHLKRAS
                     PYNTYVNVGLPPGPICSPGSKSIKAVLYPEKVDHLYFVSNQDGTHHFSTTLGEHNEAV
                     SRYLRKLKESRKQDGPESRAKTPSEQESVPGDSKPATGPNKEPDRKKRSP"
     gene            12642..13685
                     /gene="purM"
                     /locus_tag="PLM79_09305"
     CDS             12642..13685
                     /gene="purM"
                     /locus_tag="PLM79_09305"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOV86547.1"
                     /translation="MGAKFYRESGVDLDKANELVKRIQPLVRTTFHGGVIKDIGGFGA
                     MYSLNCSELKNPVLVSSTDGVGTKLRVAAWADRHNTVGIDLVAMSVNDIIVQGARPLF
                     FLDYLAMGRLNLDVVEQIVGGVVAGCKEANCPLIGGETAEMPGFYGEKEYDLAGFAVG
                     LVENSDIIDGSSIHVGEVIIGLASTGLHSNGYSLVRRILLDRMGLKPDSFLEECNRSA
                     AEELLAPTRIYVEPVLKVLKQFRISGMAHITGGGFYDNIPRILPRACRALIRKGSWEV
                     PPIFRILQEKGEIPEEEMYHVFNNGIGYILVAEPEETDDLLGLFRAMGEKAYTIGEIV
                     PREENGPPVQILP"
     gene            13812..14777
                     /locus_tag="PLM79_09310"
     CDS             13812..14777
                     /locus_tag="PLM79_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOV86548.1"
                     /translation="MEPFSAESTKTKDPRSEKIDEAISLLSHYLHGKEKALRLALTCF
                     FSRGHLLIEDLPGLGKTTLAIAIAKVLGLTFGRIQCTSDLLPSDITGLSIFNKDKNAF
                     EFQPGPIFNHLVLVDEINRATPKTQSALLEAMGEKQVTIEGRTYDLPRPFFVIATQNP
                     VEHFGTFPLPESQMDRFMMRISIGYPPRTAEKEILRGGSRRKEIYSVLPIMEKEESVE
                     IQAEIRNGVYVSDKILDYLLNVIEATRRSKYLVAGLSTRGALALIHTAKSDAYFKARD
                     YVTPENIKELARYVIPHRVLFREEYENTDREEIIQSLLKDVPVPA"
     gene            14861..15646
                     /locus_tag="PLM79_09315"
     CDS             14861..15646
                     /locus_tag="PLM79_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOV86549.1"
                     /translation="MYIIASALLSYMLVSGIFGRANLYGVQVAAHFPGEIFAGTETLV
                     QVRVANPRRFLPALLIRVSVGEQAAFFPFIPAGSDASAYVLLRFERRGRHTMPGLLVT
                     SVFPFNFFTRFRAIPGEFHVTVYPKPVRCDPALLDDRSTTLRGEISSNQAGYDSDILS
                     IRDYVSGDPPKYISWKSTAKTGRLKTKELSSIQLQQIMIDLDSMDKKHLESLISCAAY
                     LVLRYTRSNVPVGLTLDGRGIPPNTGSAHRTRLLTRLALYGPN"
     gene            15633..17546
                     /locus_tag="PLM79_09320"
     CDS             15633..17546
                     /locus_tag="PLM79_09320"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOV86550.1"
                     /translation="MVQISDALKIITYLSVITCFPSVAPFVGIPFTVAFWVLFAVAAY
                     SDYRPLVRVPRWVLNCLSLAVLALAVVRVTPEHIVEPVLDALLILIAIKLLEEKQFRD
                     YMQIQMMCMFLLIGSSFLSLSIIFLLYFLALVVLSTTALILLAYFSQDPELILQKANL
                     GKIALYAMLICSISIPMCTVFFLILPRTNYPILSFLNKSGYGRTGFTDSVVLGGVSEI
                     QEDNNVIFRVEMERLPDAKLYWRGVVMDYFDGASWRSSGGVPELGQFEGGEPIPQTVY
                     LEPYGHKYLFALDKPMSLTLRGSTPSAAGTFLHKEYIFDRLKYKTTSVASDFLSTPEI
                     EEARYLQLPEAAPAGLRELHSRLTAGKGEIDVIRTLFHFLSRGDYKYSLASLPQSETP
                     LEDFLLRQKQGNCEYFASAMAVLLRMSGIPARLVGGYRGGFYNKTGKYYLVLQKHAHA
                     WVEAYSRNRGGWLRLDPTPGSSLDPTRFYEGSLLLQLKLRLDTFNYYWNKLIIDYDFS
                     RQLAIVQGIRATIRKSDFRFDLRKARPGKEWGLVLLAGILGVVLAAAIFRYRGRSPHE
                     KVISGFLRRMDRHGYQREPGEGLEEFVARMDPSPLRERAGAFVEEFQETFYRDRPFTR
                     EKVRALEERLRAL"
     gene            complement(18361..18672)
                     /locus_tag="PLM79_09325"
     CDS             complement(18361..18672)
                     /locus_tag="PLM79_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86551.1"
                     /translation="MRSFQQNQAAHAISICFQMQHGGGIQDADRHTSPESVAKAGPSV
                     PFYTELPQVREGPLPFPEAILLPDQGPEQAQSTSVVAQAQRQAWRRREDGYALSSASP
                     E"
     gene            18928..19158
                     /locus_tag="PLM79_09330"
     CDS             18928..19158
                     /locus_tag="PLM79_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOV86552.1"
                     /translation="MEYKRITVNPHQMGGVPCLRGLRIPVATVVDMVADGLTTKEILA
                     AFPDLEDPDITEALRYAAEAVRERELPLVRVA"
     gene            19155..19526
                     /locus_tag="PLM79_09335"
     CDS             19155..19526
                     /locus_tag="PLM79_09335"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HOV86553.1"
                     /translation="MKFLVDNALSPLVADGLRNAGFDTIHVREIGLQAEEDPVIFGKA
                     IDDDHILVSADTDFAALLALWKRSKPSVVLFRRGSERRPNQQVALLLGNLPHIEESLR
                     KGSIVVFEQHRIRIRFLPISG"
     gene            19994..20203
                     /locus_tag="PLM79_09340"
     CDS             19994..20203
                     /locus_tag="PLM79_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOV86554.1"
                     /translation="MKRVFTAIIHKEDELYVAQCPEVGTASQGASVEEALLNLQEATE
                     LYLEEFPMQETPRPFLTTFEVAVHA"
BASE COUNT         4053 a         6523 c         6146 g         3931 t
ORIGIN      
        1 tcccggagga ccgatcgcac cgtacgtaag ggagacgctc cgggtgccgc cccgagaagg
       61 cacagggttc gcccttcccg caaatcgacg atccccttcg ataggggcaa ggtttgtccc
      121 gccccccggg aatcgtgctt tttcccgcct catggctgaa attgttccgt ttgggctatg
      181 gtcagaggcc aggtatggat tcggacaatc aaggacaccg acggcgactg cgggaaaggt
      241 ttttggacag cggcctggac ggcttccatg accacgaggt cctggaattc cttctatcct
      301 ttgccatccc ccggaaggac ctgaagccca tcgccaaagg cctgctcgag aagtacaaaa
      361 ccctctcggc ggtgctggac gcttcctccg agcgcctcga gaaggaggac ggcatgggac
      421 cgtactcggc cgttttcctc tccctcatgc cccggctcgt ggaacgctac cagcaggacc
      481 ggtggaaggc caagcctgtc ctttgctcca cccgggacgc ggtggcctac ctgacttccc
      541 tcctggggac ggaacgcaat gaagtgtttt acgtcctggc cctggacagc aagaactcgg
      601 tccttgccgg agagggcgtt cagcggggca gtgtgaaccg caccgccgtg tttccccggc
      661 aggtggtgga ggtggtgatc cggcaccggg caacggcggt gatcctggcc cacaatcacc
      721 cggggggaga cccgactccc tcggcttccg accgccaatt gacccggaag ctaaagagaa
      781 tcctcaacga cttggatatc cacgtccacg atcacatcat catcgcgggt cccaagtatt
      841 tttccttcgc cgaaaaagga gagctcgaat gaccggggaa atggaagcgg tcctggtaaa
      901 ggaaacgggc gccaaaggag actggaacgt cctggagatc ggcaggattg cccttcccgc
      961 cctccgctcg gaggaggtcc tggttcgagt ggaagcgtgc tcggtcaacc gggcggacct
     1021 gctccagcgc aggggtttgt atcctccgcc tccgggcgca tcctccgtcc tgggcctgga
     1081 ttttgccgga atcgtctggg aaacgggaac caaggccacg gcgtggaaga ggggcgaccg
     1141 ggtcttcggg atcacggcgg ggggcggata tgcccgttat gcggccgtgc acagggacca
     1201 cctggtgccc atccccgaaa acctgggctt cgtggaggcg gcggcggcag ccgaagtctt
     1261 tttcacggcc catctcaatg tcttcctgga agcgggactg aagcaggggg agaccctgct
     1321 cctgcacgga ggcgggagcg gcgtgggcac ggcagccacc cagctcgcct ccctggccgc
     1381 tgcccgggtc ctcgtcactg ccggaagccg ggatcgggtt cagcgctgcc tggacctggg
     1441 agcgtctttc gtcatcaact acaaggaaga ggactttgcc gcccgggcca tggaactcac
     1501 ccggggggag ggagtggacg tgatcctgga ttggatcggg gccccctacc tccagaagca
     1561 cgtgcgcatt ttgaagcccc ggggacgact ggtcctcatc ggcctcatgg gcgggaatac
     1621 cggcgacatc cacctggctc cgctgctgac caagcgcctc cggattatcg ggtccgtgct
     1681 ccggagtcaa tccgtccggg agaaggcggc cctgacggcg tctttcaaag aaaaggtcct
     1741 gccgctcctg gcctccggtg cggtgcgtcc cgtcctccac cgggtctacc ccatggaagc
     1801 cgtggaagag gcccaccagc tcatgaaatc gggggcacac ttcgggaaac tggtcctgtc
     1861 ctgggaaaaa cccggggaag cacaagggcc gtgactcccg cgaagggggg gccgggcgga
     1921 caacggagat tacgatgccg cccatgggga cgtgactccc gcgaaggggg ggccgggcga
     1981 ggaagcttgg ccgtcccagg gacggttttt atccagccga aggtcgatgg aagaatcccc
     2041 gggaacgaca atttgaggaa atccccatgg agaacgagtg gatatcgttg gagcaggtca
     2101 cctttgttcg aaatcggcgg accatcttgg acgggatcga ctggagggtt caaccgggag
     2161 atcactgggt tgtgctgggg gccaacggtt cggggaaaac gacccttctg cagctcctgg
     2221 ccggatatct ctggcccacc cagggcagcg tcgtcgtttt gggagaacgt ttcggccaca
     2281 cggacctgag ggaattgcgc aaacggatcg gatgggtggg ttcctttctc gaaatgcagg
     2341 tcccgtccac cacaaaaccc ctagacctca tcgtcagcgg gaaatacgca tccatcggca
     2401 tcttcgaggt ccccacccag gaagattatc gtgaggccca agaccttgcg tcccggctgg
     2461 ggtgcgaacg aatcctcgac tccccttacg gagttctctc ccagggggaa aagcagcggc
     2521 tcctcatcgc ccgggccctc attcaccgcc ctccccttct catcctggac gagccgtgcg
     2581 cgggcctgga cctggccgcc cgggagcagc tcctccggac cctcgacgac atgggacgcg
     2641 gcaaccccgg cctcaccatg atcctggtga cccatcacct ggaggagatc accccggtgt
     2701 tctccggcgt gctgctcctc aaggaaggcc gctgcacggc ccaagggcaa aaggaggaga
     2761 tcctttcccc ggaccggttg aaccatgtgt tcggcatccc catcgaggtc caccgaaaac
     2821 aaggccgcta ctgggccgcc atgcggttcg aagacgacgc ccaggacctg ttcgggaaca
     2881 agactccccc ttcccccacc aacctcgggg gaggaaggcg gacgggcatt agaagtgaac
     2941 gatgagggag gtgaagaggg tgtgctcgca gacgtcctgg gaaaacctct gaggtcgaac
     3001 gatggagctg cccacatccg ttcccaggcg aacctggtag gcaatgtccc agacggtcct
     3061 tcccacgccg atccccgagc ccaggctaaa gccgtagtaa gggtcgtgcc ccccctcggc
     3121 cggggccggg tcatagaatc cccctgcgcg aaggggcacc acgaagccgg gccggatgaa
     3181 aagacgctcg aagccggctc gaacctgggt cgtggcctcg atcctcgatt ttcccagagg
     3241 caggccgctc accggggaaa actctctgcc ccgggcgctt cgatagacga agtcctccca
     3301 ctgggtccgg gaaacatcca gggccgccgt cagcttgtcg gaaaagcgat accccagccc
     3361 caccccgtag gacatgggca tgtccagttc ttcgtccagg aaattgacct gacgataggc
     3421 cggatacgga agataccgga tgaccgacgt ggttcgatga gccagttccg cggtgaaggg
     3481 gctcttgaac accaggccca ggctcaggtg ttcggtgagg ttccaaaggg cgcccacatt
     3541 cacattccag ccgctgaaat cgtaacgatt tcgaacgtag tgattcaccc tgtggagatc
     3601 gcccaggatg tccctgacac ggttgcgggt tcgaatgacc tgttcccacc cgttgcgata
     3661 cagcagatct tcccacaaat tcagggtaca gcccactgca agtcgaggtg atacttgaac
     3721 ccccagggca attcccaagg cgtacagccc tccgccctgg tccactttcc atgcctgctt
     3781 caacccctcc gagtggagcc gggtgttcca ttcccgggta aaatcgtaga ggtgctgata
     3841 gttgagggac gccaccacgt tggtgtcggc caccacaagg gggcaagcgg cgctcaggta
     3901 gttgagtccc ccccgggaga cggtctgttt ctccgaagcc tccgagtgat ccggaaaaag
     3961 gttgtcctcg gaccggagaa cggcggtgcc cacggcggaa atttcggggc actccaactg
     4021 ggtaagcccc cccggattcc aggaagccgc agtggcgtcg tccgcaaccc cgatgaacgc
     4081 gcctcccatc cccagggccc gcgcaccgga gcccacggga ttgggagaag aggcaatttc
     4141 ggcgctttgc aggctctggg cccaccccgt tgcccccagg acgagaacgg tcgacagaaa
     4201 aaatacggct gccggaaaga gggtccacaa gggccggggc tttttcttgc ctggaaagag
     4261 agacatgggg tcttgtccgc gaaactcagg ccggggccat ggacacattc ctggtcttgg
     4321 tgaccccgga aaggagggtg atctttcccg cataacgctt gtagccgctc tttcttgcaa
     4381 ccagggtatg gtttccggcc gcccccgcca gcaggtactg gccccggggg ccgctcgaga
     4441 ccgaaaccgc ctcgtcgtcg atggtgaggg tcactccctc gaggggagcc ccgctgaggg
     4501 catccgtcac aatcccggtg acaaagcccg tggatgaagg cataaaaagt ctcctttcct
     4561 tgcaggtgcg ttcgcccgtc cctttccggg gaaggggcgg gccgtggcgg gaagctcact
     4621 cgaatgccgg atccggctct attgggtgag gaccttctgg aggggctcga agctccgggg
     4681 tccgttcccc gccggggctt ccacctcgga catctgctcc ccgacggccc ggacgagccc
     4741 ccaccccagc acttccatgt cggcccccag gaagacgccc gatagcggat gtcggacggg
     4801 ttccccttcc cggaaaacga tgactctcat ccctcgtttc agacggctct ctttgcccaa
     4861 atccaccgtc agtcggttgt cttggacccg aacgacggtt ccttccagga ggggcacctc
     4921 gtcgcagagc ttcaagacca ggccccggct gagctcgtgc agggacggca ggtcccgttc
     4981 ctcgccataa acatccacgg cggcgatcac cagggaggtt tccgtatcca ccaggcgaag
     5041 gtagatttcg agggtcggcc ccttttcccg gacagttccc atgagcatgc cccccgcagc
     5101 caggatcttt ccgatccgga ccgccgtatc cgcatcggcc agccccccgg ctcccagtct
     5161 ttgttcccga aggatctcct cgagacggcg ccgctccacc agggcgaacc gcccctgccg
     5221 gaccaggtcc cccaggatga gctcctccag ggcatccgcc gcggatcctc cgccgtccgg
     5281 acgtccatcg aggggaagaa gcgccacggg caaacgggaa ccgatgtccc ggaccttctg
     5341 gagacgacgc tcgaagcgca cggtcttttc agccttgttc ccgtgcaggt cccgggcctc
     5401 gatcacaaaa tcgtttgccc cttcctcgag ggaagccagg tgagccaggt gaaccctctt
     5461 gcctgcgctg cgcggcaagg ggcgaccgtt gagccggacc tcggccaccc cgctttcgtc
     5521 tcgaacgcag gcttccaggt acacctggtc gagaaacacc tgctgctgcg ccgaccagtt
     5581 gcgcagttcg atcaggggag gaaagacatc tccgcccctt tgatcccctt cgtccgggga
     5641 cggggaaacc ggggtccggg acgccgtcag ggtccttctc tcccgggaaa ggctctccag
     5701 ggggatttcc gcccgagttg cgtttcccac ccggtcccgg gcttccacaa ggacccggtc
     5761 cgaccccgga gccgggaaga ccggcaaatc caggcgaagt tcctttcccg aaggaatcgg
     5821 aacgacccgg ccgttcacga cgacctcctc gagtccggca tcgtcgtgaa cgattccccg
     5881 gattctgaac cccggggatt tccttcccga ttcccggaaa accccctcga tgccgatggt
     5941 gggaccctgg gaatccagga cgacccatcg ttccagccgg gagattcgtc cttccaggtt
     6001 ggccgcttcc accacgacgg gattggaccc gccccgaagc ttcacctgcc ggtggaacgg
     6061 gacctcccgg gccgcaaggt ccaggcggac gggctccccg ttgacggtga cccgtttgac
     6121 gaaacccggg tcccgaacga ggccctccac ccgggccaca ggggttcgca cccaggtgtc
     6181 gggatcgggg gaagacagga ggatctcggg aacggacgcc ggggtctttt cctctcgaat
     6241 cttctctttg cggaccaggt ccaggtaata ttccgccttg gcggacttct cggaacccag
     6301 ggactcctcg atctcccggg tcgcctcctc gaaacgtccc cgccgaaaat agacgatccc
     6361 cagttcccgg tggggaaagt aatccgtgaa gtgcattccg taggtccggg ctcgcctttg
     6421 atctttcccc ctgagcttca gcgcatgctt caggtccgcc tcggcctcct gccaggcccc
     6481 ggcttccgcg cgggaaattc cccggtggta gtagtgccac caacgacccc gaaaggtccc
     6541 tttcatgagt cccccggtcc tctccggggg agcccctatc ttgatgaggt ccccggtctc
     6601 gcaggcccaa agacccagga gaaggagtac ggaaagaacg gccgaaaaga tccccgccat
     6661 cctcatcctc cccgtgcccc gggaatttcc tcgacccgcc accccggctg cagccgagcc
     6721 tggggagaga cgagtacggc cgtagacgac ccgtcggaca cgtccaggac caccagttcc
     6781 gctcccttcc cttcgggttc catgacccgc aggcgcatat cggttgcgat tccgcaaagg
     6841 ctcccggcat tgagagtcaa ctgggaaccc aggacttccc ggatgcggca ccggacggga
     6901 taggcctccg tgaggcgttc ccggaactgc cgggaggttt tcctcacggc agccacggga
     6961 tcttccccgc cgggaaccag ggcgctcgaa tgggccatca cccgagtggt ctccgtatcc
     7021 acggccttga ggttcagttg gacccggccc tgatggcgca gaatcgagcc cgtgcagagg
     7081 actcgggccg aaagaatctt ccccaggcga agagcggtct ggggctccgc cagctgggag
     7141 ctgctcagtc gcaattcctc gaggagccga tccagcagtg cccgttccac cacctgaata
     7201 cgagggcatt ccgccagttc ccccgcgatg agctgggaga aaaacgcgtc ctccccctcc
     7261 cggggagacg gtgccccacg aggctcgaga tcgaggaaga acagggaaag gggccgggaa
     7321 gtccatgggt ccgcccccga gagcgaccgg ggtccctccc cgtaggcctg caccagttgg
     7381 gccaccaggt cgtcgatccg cttcttttgt tcttcgttcc tgcgtgcttc cttctcctgc
     7441 cgacttcgcc ggtacaatcc aaaattgacc acatcgtcgc agttgaccga ggcacccttc
     7501 tcgtaggaat ccacggcacc ctccagccgg cccagccgtt ccagggttag gcccctggcc
     7561 gtgtagaccg ggagaaacgc gggatctgcc tgcagcgccc ggtcgaattc ttccagggct
     7621 tcgttcaacg agccccgcat cagctcgatc cgccccaggt ccaggtggat ctccgcccgc
     7681 tgccatggct gcagtttctc ctcgtttgcg atcttgcgga aggagagccg tgcctcgtcc
     7741 gtgttgcccg aggccagggc gaaccgtgcg gccagaacgt ccaggtaggg atcgtccggc
     7801 tctcgggatc gagccgattt ccacgcttct cgggcttctt ccacgtttcc ctttgccagg
     7861 gccagggccg aaagtccctg gtagtagacg gcgttcttgt ccgtcatgga ctgaaggagg
     7921 ccctcggccc gggagatctg acccgtatga aggagatgga aggcttcctc gccgggcctc
     7981 cggagggcat tcggcccggg gctttccctc cggatgccca ggacaaagag gccgaggatt
     8041 aggaccaggg ccgaaaccag ggagagaaca ggccaccgcc ggaccttatt cctccagaac
     8101 cgggagagaa aagaaaacgc cccggcgggg gcgacgcccc ggtccggaaa cgtctccaaa
     8161 ggatccccgt ggagcacata ggaggcccag gtaagggtct ccttgccgta gtgttcaatg
     8221 gaccgctgac gggcatagcg cagggcctcc cccacgggac tcccttccag gaggcgcgta
     8281 tagaattcgc gggcaaaggc ggagctcgat cggtcgaaaa catcgagcct cgacccgatg
     8341 taatggcgaa cccccgactg gaggaaaccg ccggccagcc cgtggagccg ctccccggaa
     8401 gatccgcccg ggtcgcggac cgatcggcac gcgttggcaa aaaccagccg gggaagcctc
     8461 ttcctccggg ccagccccag gagccgtccc gtttcaaact tgccgtcgga caggaggaat
     8521 ccgctgaggg agggatccgc cccgtcgaag tcccaatggc ccgcataatg aaagaggtcg
     8581 tactggggca gttgatcctc gaggaaggcg caggtggcct cccgactgct gaggtacacc
     8641 cgcagaccgg gaatgtcttt tcgggtgaaa acccttcgca cggagatccc ttcggcccgg
     8701 gcggccgtca ggtctccccg ggggtccgcc acgatcagca ctcccacccc ggccgggcgg
     8761 gactttcctc gaggaagatc cgtcgacgga gaggaaagga ccatccttcc catgctgtac
     8821 ttgagacaga agaagctttc cccgtcgtgc agcaattccc agggaatgtg caccagggcc
     8881 gggtcgatcc cgcacaggac ggagtcgggt cgccggtcga ggacctcctt ggccaggtct
     8941 cgagggacca ggatctcgta gaggtttgtg ccgaggtttt ccagtcgtcg gacctgttcc
     9001 ttggaaaccc ttcccctaga atccgttccc aagagacact cgtcgatttc ttccaccaac
     9061 tcccggacct cccgttcttg aaacggaacc ctgagggacc tcggtactcc gtcctctctt
     9121 ttcccccagg aggcgtcgat tcgcaggatg tcgtcttcca tttggaaggt cagccggcag
     9181 gaggccccgc ccgattccgg cccctcccgc cccgggggga gcgtccgctt gaggtccgcc
     9241 tcctggaagg ggtcccacag gatccgatag acgtccacgg gcagggtttt ccctttgagg
     9301 gttttggccc ctacgtattc gtgggcgatt tccggagcat cccggacggc atccaccagg
     9361 gaccgggaaa caaggatgtc ccctttcttg gccagggaac acagtcgagc cgccgtgttg
     9421 gggacgtctc cgtggatgtc cgtttcttcg acgatcgcca gcccccagtg catgccgatc
     9481 ctcacccgga tctgttcgag gggctcccgg cccttattct cggaggccag tctgcgctgg
     9541 atttccgccg cagcgagggc cgcccggacg gggtcccgga aggagaccat caaaccgtcc
     9601 cccagggtct tggtgacggt gccgccatga gcctcgatga cgggccgcag gaggtcatgg
     9661 tgccgctgaa gcatgagcct gccctccagg tcccccagct tttcgaagta gtccgtggac
     9721 gccacgatgt cggtaaaggc aacggcaacg gggcgctgaa accgatcccg tatgctccgg
     9781 tcaaggtcct ctctcctgcg caggaattct tcgatggagg agacggggct tccggggtca
     9841 tcgtccttgg ggagatcatg gagagtcatg ggaattcctc ttcatcaccc tttcccgaag
     9901 gggcaacggt tttggctcga gtgcagcttc atcccggcga gttcccacat gcagctccct
     9961 cgagacggtc gaaaccccgg tcctcgtcgg ggtgacgccc ggccaacggg attgtccgaa
    10021 aggggaaccc gccctgcatc gaccttttcc cccggggaac ggatgcggtc atccttcagc
    10081 ccctggatgg attcgatggg cccctcctcg aaaacaggga cgacaagcat tggatttgtg
    10141 gggtatgaat gtgccgcccg tttccggacg catgggagaa cgggcgaaat gccccggcct
    10201 ttccccttgt tacccggttt tgcgaattcg ggagcctgca atttcccgag gaatcaagtc
    10261 gttacgatga atagagctat taacggaacg atacacttga attgggacga ttattagcag
    10321 agtcagcggc cgcaatcaag gaccgaaatg acggggaagg acccgggggg cgttgggtgg
    10381 aaggaaaggc cgccgaaggc cgggcgaacg actaacacct cccggaatcg ccgctcttgc
    10441 ggcgagcgta ctgttcctgg acaaagcgga gggattccgg agtgtccaca ccgcagacct
    10501 cctcttcggg ggccgtcacc cacccgacgg aaaggccgtc ccggctcatc cattccacca
    10561 ggtccgtgag aaagaactct tcgatggtga cccattccgt cccccgctgc ttttgaaccc
    10621 ggtgggggcg cgtggccagg atgctcatgt agtccagaag caccgggcga cggaccgcgt
    10681 agaccccggc attgcagtat cgaagggccg cctgtcgatc tggggggaag gcatgccagt
    10741 atttccattc gacgatgccc gtcacccgat cctcttccat ctcgagcatg ccgtattggg
    10801 ccctgtcccg gggctcgaag gccaaaatca tcatctcgtg ccggtccagt cgggtcagaa
    10861 gctccccgta ggtccgggac cgaatcaacg gcacatctcc catggtgatg agcaccgagt
    10921 cctcccggac cttctcgaga aaggggcggg ccgaaagcag ggcacctccc gtgccgttgg
    10981 tgctgggctg gaacaggtat acggccccct gtcccgccgt tgcctccttc acctgccggg
    11041 cgcagtggtg gaccacgatt cccttgggac cgggcggcag gttggagagg acttcgagga
    11101 gcaggggacg ctcgccgttg tagggagagc ctccgggaat cagcggcagc agggtcttgt
    11161 ttccctggta ccccaccatg cgggttcctt tgcctgcggc aaagaccacg gcggcggcgg
    11221 cggaatcgcc ggaaaaccgc cgggcgggac ggggggactg aggggatcga gtgggattca
    11281 tgggcttcgt ttcttccggt caggttcctt gttgggtccg gtggcgggtt tggagtcccc
    11341 cggcacgctt tcttgttccg aaggcgtctt tgcccgagac tccgggccgt cttgtttccg
    11401 ggattccttc aacttcctca ggtagcggga gacggcttcg ttgtgttccc ccagggtggt
    11461 ggaaaaatgg tgggtgccgt cctggttgct cacaaaatag aggtgatcga ccttttcggg
    11521 gtacagcacc gccttgatgg acttggagcc cgggctgcaa atgggccccg ggggaagacc
    11581 gacgttcaca taggtgttgt acgggctggc ccgtttaagg tgggccgacg tgatgggacc
    11641 cgtgaagccg tccaggtcgt acacggccgt gggatcgctc tggagcggca tgcccagctt
    11701 gagacggttg aagaacaccg cagcaatcac gggtcgttcc gcatccacca cggcctcctt
    11761 ttccaccatg gaagccagga tgaccacctc acggacgctc agccccgact gggaaacccg
    11821 gtctttccat tcctcgggaa agcgggtccg gaactgctgg accatggctt tgaggatgga
    11881 agaagccgtc gaggaccggg gcaaaaggta ggtatccgga tagagatagc cctcgaggct
    11941 gggggaatcg aggcccaggc tgcgcacgaa atccgggtcc gcagaaagcc gcaagacctc
    12001 gtcgggggaa gccaggtgct tctctccgat gatcctggcc gtatctcgga gggtggatcc
    12061 ctcgggaatg gtgaggcggt gcaggaagac ccggccgtgg accaggtgat ccaggacctg
    12121 ctcgggcgtg agaagagttg aaaaggcata ttccccggcc ttgagcttct ggtcgccttt
    12181 tcgaatccaa cacaggaccc tgaactgtac ggcgtccgca accacgccgt tttcccgcag
    12241 aagacgggcg atccccatgg ctcccatgcc cggccggacc tccacccacc ggacgccggg
    12301 ctgcacgctg gggggaagcc tcaggaaaag ccagaaccgg aagcagacca acccggcggc
    12361 aacggcggag agtccgatca aggaaaccag gcaaaaacag ccccacagaa cgtagcgacg
    12421 acctcgaccc attcccacct cctgccgcat ggatttgtac cacgaaagaa cttcggggac
    12481 aacgaaggcc tttcctccac tctcgtcctc ggagaaatgg aagcgaggga ttcaaaaagc
    12541 cgacccggga tccttcaaga atgtttggca gacgttcgat ccatgttata gtttacgtgc
    12601 ttgacgacaa aagaccgagc gcaaggaggg gaacgcgcct tatgggagca aagttttatc
    12661 gggaatccgg ggtggacctg gataaggcca atgaattggt caagcgaatt cagcctttgg
    12721 tgcgaaccac ctttcacgga ggggtcatca aggacatcgg cggcttcggc gcaatgtact
    12781 ccctgaactg ttccgaactg aagaaccccg tactggtgtc ttccacggac ggggtgggca
    12841 ccaagctccg ggtggccgcc tgggccgacc gtcacaacac cgtggggatc gacctggtgg
    12901 ccatgagcgt gaacgacatc atcgtgcagg gggctcgtcc tctcttcttc ctggactacc
    12961 tggccatggg cagattgaac ctggacgtgg tggaacagat cgtcggcggg gtggtggcgg
    13021 gctgcaagga agccaactgc ccgctcatcg ggggcgaaac cgcggagatg cccggcttct
    13081 acggagaaaa ggaatacgac ctggcgggat tcgccgtggg actggtggaa aacagcgaca
    13141 tcatcgacgg atcgagcatt cacgtgggag aggtcatcat cggccttgcg tccacgggcc
    13201 ttcattccaa cggctacagc ctggttcggc gcatcctcct ggaccgaatg ggactcaaac
    13261 cggacagctt tctcgaggaa tgcaaccgca gcgcggccga ggagctcctg gcgcccactc
    13321 gcatctatgt agaacccgtt ctaaaggttt tgaagcagtt tcggatctcc ggaatggccc
    13381 acatcacggg agggggattc tacgacaaca tcccccgcat tcttccccga gcctgccggg
    13441 ccctcattcg gaagggttcc tgggaggtcc cccccatttt ccgcatcctg caggaaaagg
    13501 gggaaattcc cgaggaggaa atgtatcacg tcttcaacaa cgggatcggg tacatcctgg
    13561 tggcggaacc cgaggaaacc gacgacctcc tggggctctt cagagccatg ggagagaagg
    13621 cctacaccat cggagagatc gtgccccggg aggaaaacgg acccccggtg cagatcctgc
    13681 cctgacccgg tcaacatccc gcaaagaaga cgggtcttcc cggaaccccg ggcctcccgg
    13741 ggtgttgaac gcccgcgacc ccgtggggca acccacgaaa aacaaccact ttccttgtaa
    13801 ggccggcccg gatggaaccc ttctccgccg aatccaccaa aaccaaggac ccccgaagcg
    13861 aaaagatcga cgaggccatc tcgcttctct cccactacct tcatgggaaa gaaaaggccc
    13921 tgcggctggc cctcacctgt tttttctccc gcggccacct cctcattgaa gacctccccg
    13981 ggctgggaaa aacgaccctg gccatcgcca tcgccaaggt cctgggactc accttcggaa
    14041 gaatccagtg caccagcgat cttctgccgt cggacatcac cgggctgtcc attttcaaca
    14101 aggacaagaa cgccttcgaa tttcaaccgg ggcccatctt caaccacctg gtcctggtgg
    14161 acgaaatcaa ccgtgccact cccaagacgc agagcgccct gctggaagcc atgggagaaa
    14221 agcaggtcac catcgaaggc aggacctacg acctgccccg ccccttcttc gtcatcgcaa
    14281 cccagaaccc cgtggaacac ttcggcacct tccctctgcc cgaatcccaa atggaccggt
    14341 tcatgatgcg aatcagcatc ggttacccgc cgcgaacggc cgaaaaggaa atccttcggg
    14401 gaggaagcag acgcaaggaa atctactccg tccttcccat catggagaag gaagagtccg
    14461 tggagatcca ggcggagatc cgaaacgggg tgtatgtctc cgataagatc ctggactacc
    14521 tgctcaacgt catcgaagcg acccgaagaa gcaagtacct ggtggcggga ctttccaccc
    14581 ggggggccct ggccctgatt cacacggcca agtccgacgc ctacttcaag gcccgggact
    14641 acgtcacccc ggaaaacatc aaggaactgg cccggtacgt gattccccac cgggtgcttt
    14701 ttcgggaaga gtacgaaaac accgacagag aggagatcat ccaatcgctg ctcaaggacg
    14761 tgcccgtccc ggcgtaatca agatcaacaa agcggggtac ctttacatcg tcctcaccat
    14821 cctcatcggc ttctccgcca tcaacacggc caacaatctc gtgtacatca tcgcttcggc
    14881 cctgctcagt tacatgcttg tgtccgggat cttcgggaga gccaatctct atggtgttca
    14941 ggttgccgcc cattttcccg gcgagatctt tgccggaacg gagaccctcg tccaggtacg
    15001 ggttgccaac ccgagacggt tcttgcccgc cctgttgatc cgggtgtccg tgggagaaca
    15061 ggccgccttt tttcctttca tcccggccgg gtccgacgcg tcggcgtatg ttcttctgcg
    15121 ttttgaaagg cgcggcaggc acaccatgcc gggcctcttg gtgacctccg tttttccgtt
    15181 caatttcttc acccgattcc gtgcaattcc cggcgaattc cacgtgacgg tctaccccaa
    15241 gcccgttcgc tgcgatccgg ccctgttgga cgaccggtcc acgaccctca ggggagaaat
    15301 ctcctccaac caggcggggt acgattcgga tatcctttcc attcgggact acgtgagcgg
    15361 ggaccctccc aagtacatca gctggaagtc cacggccaaa acggggcgcc tcaagaccaa
    15421 ggagctgtcc tccatccaac tgcagcagat catgatcgac ttggattcca tggacaagaa
    15481 gcacctcgag tccttgatct cctgtgccgc ttatctggtc ctccgctata cccgctccaa
    15541 cgttcccgtg ggcctgaccc tggacggccg ggggatcccg cccaatacgg gatcggctca
    15601 ccgaacccgg ctgctcacga ggctggcact ctatggtcca aattagcgac gccctcaaga
    15661 tcatcaccta cctgagcgtg atcacctgct tcccctcggt ggctcctttc gtgggaatcc
    15721 cgttcacggt ggctttctgg gtgctcttcg cggtggccgc ctactcggat taccgtccac
    15781 tggtgagggt tccccgctgg gttttgaact gcctgtccct ggcggtcctc gccctggcgg
    15841 tggtgcgcgt cacacccgaa cacattgtgg aaccggtcct ggacgccctc ctgatcctca
    15901 ttgccatcaa actgctcgag gagaagcagt tccgggacta catgcagatc cagatgatgt
    15961 gcatgttcct gctcatcggg tccagcttcc tctccctgag catcatcttc ctcctctact
    16021 ttttggccct ggtcgtgctc tccaccacgg ccctgattct gctggcctat ttttcccagg
    16081 accccgaatt gatcctgcag aaggcaaacc tgggaaagat cgccctctac gccatgctca
    16141 tctgcagcat ttccatcccc atgtgcaccg ttttcttcct cattcttccc cggaccaatt
    16201 accccatcct cagctttctc aataagagcg gctacggccg gacggggttc acggacagcg
    16261 tggtcctggg aggggtttcg gaaattcagg aagacaacaa cgtcatcttt cgagtcgaga
    16321 tggagcggct tcccgatgcc aagctctact ggaggggggt cgtcatggat tactttgacg
    16381 gagcttcctg gagaagcagc ggcggcgttc ccgagctggg gcaattcgag ggcggagaac
    16441 cgatccccca gaccgtctat ctcgagccct acgggcacaa gtacctgttc gccctggata
    16501 agcccatgtc cctcaccctg cggggctcca ccccttccgc ggcgggcacc ttcctccaca
    16561 aggaatacat cttcgaccgt ctcaagtaca aaaccacctc cgttgcctcg gacttccttt
    16621 ccactcccga gatcgaggag gcgcgctacc tgcagctccc cgaggcggcc cccgcgggtc
    16681 tgcgggagct tcactcccga ctcacggcgg gaaagggcga gatcgacgtc atccgaaccc
    16741 tctttcattt cctctcccgg ggcgactaca agtactccct ggcgagcctt ccccagtcgg
    16801 aaacccccct cgaggacttt ctcctgcggc aaaaacaggg caactgcgag tactttgctt
    16861 cggccatggc ggttttgctg cgaatgtccg gaattccggc ccggctggtg gggggctatc
    16921 ggggcggctt ttacaacaag acgggaaaat actacctggt gctccagaaa cacgcccacg
    16981 cctgggtgga agcctacagc cggaaccggg gaggatggct gcgcctggac cccacccccg
    17041 gatcgtccct ggatcccacc cgtttttatg agggaagcct tctgctccag ctgaaactcc
    17101 ggctggatac cttcaattat tactggaaca agctcatcat cgactatgat ttcagccgac
    17161 agctggccat cgtccagggg atccgggcca cgatccgcaa gtccgatttc cggttcgacc
    17221 tgcgcaaggc ccggcccgga aaagaatggg ggctggttct gctggcgggg atcctcgggg
    17281 tggtgctggc ggcggcgatt ttccgctacc ggggccgaag cccccacgag aaggtgattt
    17341 ccgggttcct gcgccgcatg gaccgccacg ggtatcagcg ggaaccgggc gaaggcctcg
    17401 aggaattcgt ggcaagaatg gacccttcgc ccctgcgaga acgggcggga gcctttgtgg
    17461 aggaattcca ggaaaccttc tacagagacc gaccgttcac ccgagagaag gttcgggccc
    17521 tggaagaacg cctgcgagcc ctctgaatct ccggggtcga ggcttttgcc cggccatcgg
    17581 ggaccaggct cccggcaccg accctctgcc ccggactccc ccccctttgc ccggctcccg
    17641 ggggcttggg atcgggcacc cttcgacacc cccggcgcaa tcccggcgat cacgccgccg
    17701 ttcgtaccct ggacctcgga gcgaagcgcg ccacgggaaa agacccctcc cccgccggct
    17761 cgccggccaa gcccggagcc gtcccctccg agatcgagcg catgatgcgg acccccaaga
    17821 ctccgctgga accgatagcc cggttggact acaataggcc ccatgaagac aagcacggat
    17881 ctcatattag gttgcgccca aggtcgctct gacatggtga gtgacgattt tgagcaaagg
    17941 gctgaatcct gtgggtgggc agtctcggct tgagtgcaac ttcataccgg atgccacatg
    18001 gccgccgggt ccgctcgatc ctttggatag acatcgtggt caaagaaggt cttttgttgc
    18061 cttttgcgcc tgaacaaggg acagcctccg ccgagagata ataccaacaa tctccgaggg
    18121 ttatcctatc agaaaccggt tgcgcgtcaa tcgttaaacg gcacttttat caacacttct
    18181 caatagatag cccccaaaag gggtttttac gaatccatca tctttggatg atgccgatcc
    18241 cggcggtttg ttcttcagaa aaccctggaa ccggcgtgac caatgagcat aggtcttgag
    18301 cgtctttccg gagtaatgcc gggtcttgat ctcggaggca agctcggcta tcagtttatc
    18361 tcattcgggc gaggctgagc tgagggcata gccgtcttcc cggcggcgcc aggcctgccg
    18421 ttgcgcctgc gcaacaacgg aagtgctctg tgcctgctcc ggtccctgat ccgggaggag
    18481 aatcgcttcg ggaaaaggga gaggtccctc ccggacttgc ggaagctccg tatagaatgg
    18541 tacactgggc cctgctttgg ccaccgattc cggactggtg tggcgatccg catcctggat
    18601 tccgcctccg tgctgcatct gaaaacaaat ggaaatcgca tgggctgcct gattttgctg
    18661 aaacgaacgc atcctgtacc gcctctttga ctactcgaca ggtcacaaga tcctgaaggg
    18721 tgagacagat agcggggata aagacaaggg ctaacgaagg catgaactgg acggcgcggc
    18781 caggtgttct tgcagaagct tatcttgtaa aatggctgtc cgtaccattt tagggccgcc
    18841 ggttatgccc gacgttgggc tggtggaaaa ggcacggaac acagggtaga attcgaattg
    18901 atggtccaca atttgggggc agtcacaatg gaatataaac gcattacagt aaatccccac
    18961 caaatgggcg gagtaccgtg tttacgcggc ctcaggattc ccgtggccac agttgtcgac
    19021 atggttgctg acggtctgac aaccaaggag atacttgcag catttcccga ccttgaagat
    19081 ccggacatca ccgaggcgct gcggtatgca gccgaagcgg tcagagagcg tgaattgcct
    19141 ttggtcagag tggcatgaag ttcctcgtcg acaacgcgct ttctccattg gttgcggacg
    19201 gtttgcggaa tgctgggttc gacaccattc atgtgcgaga aatcggcctt caggctgagg
    19261 aagatcccgt aattttcggc aaggcaattg atgacgatca cattcttgtt tctgctgata
    19321 ccgactttgc cgcattgctt gccctgtgga agcggtccaa accttctgtc gtcctgtttc
    19381 gtcgtggctc ggagaggcgc ccgaatcaac aagtggcgct tcttctggga aatcttcctc
    19441 acattgaaga gtctctccga aagggcagca ttgttgtttt cgaacaacat cggataagga
    19501 ttcgattctt gcctatttcc ggttaaccct tgcccaaccg agcgctcaag ctgaccacca
    19561 accgcatgag cattttcggg agcctcacgg cgtggttgct gcggttggtg gcggcttata
    19621 cgaagttgca ttcaagccga aactgccaat tcgcaggatt cagccctttg ctcagaatct
    19681 tcactcagca tgtcagagcg acctagggcg caacctggta ttagttcgcc attggcggcc
    19741 gcgaagcggc gtcgccaatg cggcgttgcc cctatcgggt ccctgtcctg gccggcgccg
    19801 cctgcggctc tgccaaccag gccattggcc cggatgtcac ttgcgtgcat gcgccttcgg
    19861 ctccgcaagc ttcgcgccac gggtcaacgc cgcattatct attcgtaggc cgtacataat
    19921 cgttgatatg tcactctgaa atatatagaa tacatactga cttagctcaa ctagctagct
    19981 cggaggtatt aacatgaaga gagtctttac cgccatcata cacaaagaag atgagttgta
    20041 tgttgcgcag tgccccgaag tcggtaccgc tagtcagggt gcatcggtgg aggaagctct
    20101 gctgaatctc caggaagcca cggaactcta tcttgaggaa tttccgatgc aagaaacgcc
    20161 gcgtcctttc ttgacaacgt ttgaggtggc tgtccatgct tgaacttccg tgcctttccg
    20221 gtaatcaagc gatcaaagtg ttcgaggctc tgggcttcat agtcgttcga caaagaggga
    20281 gtcacgtcgt tttacgacgc gccgatcgtg gatgtgtcat tcccattcat aaacaactcg
    20341 cgacaggaac actccggagc gctatcaaac aagcaggcat cactgtcgaa gaattcattc
    20401 acacatacaa gagcagataa cacgtcattc gaccggactc cgtgaaacgt ggttgctttt
    20461 tctcggtacg ccacaggccg gagcccgtca attccaccgt tggcgccgcc tgcgtcgtcg
    20521 ccaatgcggc gttgccccta tcgggtccct gtcctggcca gctccgcctc cggctctgcc
    20581 aaccaggcca ttggcccgga tgtcactcgc ttgcatgcgc cttcggctcc gcaagcttcg
    20641 cgccacgggt caa
//
LOCUS       DAOOVC010000059        19987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1823_length_19987_cov_5.823048, whole genome shotgun sequence.
ACCESSION   DAOOVC010000059 DAOOVC010000000
VERSION     DAOOVC010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19987
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_19987_cov_5.823048"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PLM79_09345"
     CDS             <1..442
                     /locus_tag="PLM79_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86555.1"
                     /translation="PNLTVLENLELGAYYRTSREVSAELKRVYSLFPRLEERQRQKAG
                     TLSGGEQQMVALGRALMSRPRLLLLDEPSLGLAPLMVEEVLEAVQRMNREGATILLIE
                     QNAMAALRVAHYGYVIETGRIVLEGEARDLLEDQRVRRAYLGEG"
     gene            512..1876
                     /locus_tag="PLM79_09350"
     CDS             512..1876
                     /locus_tag="PLM79_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOV86556.1"
                     /translation="MKQMSFFQKPTPGKGGAPAPLAERMRPRTLGEFVGQEHLLGPGK
                     ILDRLLRNRRMQSLVFWGPPGSGKTTLARILADQSDAHMIALSAVLAGTKEIREAVGE
                     ARERWKRLGKRTLLFMDEVHRLNKAQQDSLLPHVEDGTFWFLGATTENPSFEVIRPLL
                     SRTQVLVLEPLDEESLRAILRRAMTDRERGLGAYPAEIAPEAEEYLVSAVGGDARVLL
                     NVLEVAVTTTAPGGDGIRRLDLDSVREAVQRRAVHYDKGGEEHYNLISALHKSLRGSD
                     PDAALYWLARMLRAGEDPLYIARRMVRTASEDIGLADPRALEQAMAAVRSFEFLGSPE
                     GELALAQAAVYLALAPKSNALYTAFKKARAAAEESGDAPVPLPLRNAPTELMKRLGYG
                     RDYLYPHDHPEGWVPENYLPDGLEDREFFHPTRRGWEKRFGEELNQRRQRVRHKNRSA
                     KAHD"
     gene            1869..3407
                     /locus_tag="PLM79_09355"
     CDS             1869..3407
                     /locus_tag="PLM79_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86557.1"
                     /translation="MTEENRSAGEGHPETGGKETPSPKPARERNVGPEAIKPFRLVKY
                     LSLTSLMVILVCTMFLSGFISQRAKTILVQKSEQYALLIAENLNHQVFYQFTVPTLIT
                     EGEIRLSRPNQFERLDRVVRNTIQGFSVEQVNIYDPEQILSYSTDPQRVGMKGDIGDL
                     FERAWREETVSVLSGEGSSFLGFEWRAGARKLTAYLPMWEERSMTQMRGKVLGVFEIT
                     LDVTDDYKAIHRFQWIIVLSFLGFVGILFVTILLIAARAEKIIAARAAERLKLEEQLH
                     QTERLAALGEMIAGVSHEIRNPLGIIRTTAELLHGRLENDRQKRLSSIIVEESTRLND
                     ILTEFLDFARPKALRVQSCRLEEVLDRNLEVMEPECQRLGIQVERDYRTGPYGMEADA
                     DLLYRAFVNLLANALQAMPQGGRLRLETSFANGRDGSPHIELRIQDSGHGIPAELKKK
                     IFNPFFTTREKGTGLGLAIVRSIIDGHNGDMEVVSEEGKGTTMIVRLPLVQPNREAAG
                     EESR"
     gene            3414..4907
                     /locus_tag="PLM79_09360"
     CDS             3414..4907
                     /locus_tag="PLM79_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV86558.1"
                     /translation="METVLIVDDERNYLLVLEDLLMEEGYEVLTADSGTRALEVAREH
                     ELDAVISDIKMPGMDGMALLEWFHDHKPDLPVIMMTAYGSMEKAMEATRRGAFDYIFK
                     PFKNEQLKFTVRKAIDHYHLLCRNRDLTRELQERYQFGNIIGKSIGMQRIYQLIEKVA
                     PAKATVLITGESGTGKELIAKAIHFNSPRRDQPFITVNCGALPETLLESELFGHERGA
                     FSGAVAQRKGRFELAHEGTLFLDEISEMSPPLQVKLLRVLQEMEFERVGGSQSLKVDV
                     RVVAASNRNLKEEVAAGRFRSDLFYRLNVVHVQLPPLRSRKDDIPLLVTHFLKKYPPP
                     GGRPIRMDSEAMRLLLEYPWPGNVRELENVIERAVILCNGEQILAKDLPSEIREPLPG
                     GKPEPGGELPGVGEIPSRAEETGGIDAGDRLPSSGLKPRQIKALEFVRAHGFITNRYY
                     SQINDISERQALRELNELVDGDLLHRLGKGRACRYVPAGDSPPETSS"
     gene            5920..6444
                     /locus_tag="PLM79_09365"
     CDS             5920..6444
                     /locus_tag="PLM79_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOV86559.1"
                     /translation="MRKSRIILGFLLVTVLGFGTVAPAQSADDIAKHPSCKYCGMDRK
                     QFAHSRMLIEYDDGTSVGLCSIHCAAVDLSLNIDKTPQSIRVGDYKTKKLIEAEAATW
                     VLGGNKPGVMSKRAKWAFEGKEDAQGYVKENGGELVVLDDAMKASYEDMYSDTKMIRE
                     KRKMMKSKQGEPKK"
     gene            6751..7230
                     /locus_tag="PLM79_09370"
     CDS             6751..7230
                     /locus_tag="PLM79_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOV86560.1"
                     /translation="MKKWIWIFLVWGLWTGGPGCMAGDTDPVKPTPRDKCPVCGMFVA
                     KYPDFIAGIKYPDGSYAVFDGAKDLFKFFQNVSKYHPSRKVENIVYVWVTDYYSLEPI
                     DGKKAYYVVGSDVFGPMGRELIPFEKEEDAREFQSDHGGTGIFSFDKVTPELIKGLD"
     gene            7233..7541
                     /locus_tag="PLM79_09375"
     CDS             7233..7541
                     /locus_tag="PLM79_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86561.1"
                     /translation="MFERKSHRFFAWLGVGLVLMGFMGIHAAYRRNADTPMLRGKGQM
                     VRRLALTDLCLFTDARYARNPVLADLNTPFQDCPLSLEHFPSGSLLPVPPHLGVHPAR
                     "
     gene            7546..8676
                     /locus_tag="PLM79_09380"
     CDS             7546..8676
                     /locus_tag="PLM79_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOV86562.1"
                     /translation="MRILEKQKNLVDFTLSSLLRRKGKNTALLVVYTFMIFLLASVMF
                     FTGSIKKEAALVLEGAPEITVQRLVAGRHDLIPAAYLEAVREIRGVRSAAPRLWGYYY
                     DFTSGANFTLMVDESLKDSPGVVHVGSGVLRTLMSPEHGTIPIKASDGSFHFFEIHHV
                     FPAESDLVASDLVLMCEQDYRKVFSIPDGFATDLVLEVRNPKELTTIAGKITQLLPDT
                     RPILRDEILRTYEAVFDWRGGVLVVILWGSVLAFVIFAWDKATGLSAEEKREIGVLKA
                     VGWETSDVLVMKFWEGMIISLSSFLTGVVLAYLHVFFSSSVLFEPVLKGWSVLYPDFR
                     LTPFIDGTQLAGLFFLTVVPYTVTTIVPSWRAAVIEPDSVMR"
     gene            8692..9363
                     /locus_tag="PLM79_09385"
     CDS             8692..9363
                     /locus_tag="PLM79_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86563.1"
                     /translation="MIELADVKKVFNLGRPNEFTALGGVSLSIELGKATAFKGPSGSG
                     KTTLLSVVGCMARPTAGRVRLLDREITSLPERFLTEIRRKTFGFIFQQLNLIRGITTL
                     ENVMIPAYPNGERYSELRRRALELLDLFNLGGKAESRVEWLSGGEAQRVAIARALVNH
                     PSILVADEPTAHLDTRLSIEFMGIMKDLKDRGKTILIASHDPVVFESEGIDVVVEMRD
                     GRIVS"
     gene            9373..10371
                     /locus_tag="PLM79_09390"
     CDS             9373..10371
                     /locus_tag="PLM79_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86564.1"
                     /translation="MLLHPSILAILVGALLVGGMILFSVHHAARILRYWDLAGGSELQ
                     LELERGTYLVSTVMSYALGFQVLSLFLFIFTVDDMSPLFVGAMCAAGTLNANAWGYPT
                     LILKVVNSLSAGLWLILNYTDNRGYDYPLIRIKYKMLLWIAPGMILETILQSAYFLDL
                     KPDVITSCCGTLFTSEASGAAGGILVLPRQWVEAAFYAAMGGTLAAGVFTWAKGKGAY
                     LFSGFSCGAFLLGVLSLISFISLYYYELPTHHCPFCVLHPEYHYVGYGLYGTLLAGAV
                     TGMGTGVIEPFREVPSLAETLPGIRRALALVSVLSYAGFVGMVTYQILFSNLKMGD"
     gene            complement(10421..10882)
                     /locus_tag="PLM79_09395"
     CDS             complement(10421..10882)
                     /locus_tag="PLM79_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86565.1"
                     /translation="MRKKTGFFALFCMLVGAAALPCPGFSLDEKSVSTLSTAFPVETG
                     TRSGVLLAQKPEGPPSYAAVAGILRESCASCHSGRRPAGGLHLDAYGPMMKGGKGGPV
                     IVAGDPAKSELIRRVKGTSTPRMPKGGPPWLSPEEIGLLEAWVLAGAPEGK"
     gene            11297..13357
                     /locus_tag="PLM79_09400"
     CDS             11297..13357
                     /locus_tag="PLM79_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOV86566.1"
                     /translation="MEGFFEARSVAVVGVSANPGNLAQAIVYNLLQFWFQGLVYLVGP
                     HGGSFAGHKIYPRVRELPEPVDLAAILVPASAVPEVLRDCGEKGIKRAVLMSGGFREL
                     GDERRALEEEVVGILGAYGIRMIGPNCLGIMNRRSGLAVPFMTFQATMPVGGVAVVSQ
                     SGGVAAMMVNCLEAENLGFSKFASIGNKLDVNEVDLLEYLVRDEETRIVFCYLEGIAD
                     GRRFMELASGSSKPVVLHKSNRGRAGSVIARSHSASLSADDRVLDAALRQSGVLRVFD
                     QREAMTAIKGLVLPPMRGNRLAVVSRSGGHAVMAADAADDLGFELPPFPESLLRTVQE
                     RSRARVIRFHNPLDLGDLFDMPLYHAVADETLARDDIDGLLFIHNYQGVFEQEASRQV
                     IAGLGSLIEKHRKPLAVCVFTTKPELDRTREKSHFPIFTDPREALLALAGNREHHRER
                     PAPFSHLRPPDVFPDRVKKLLDGLPEGPIGAGVLGEILSAYGLFSVPWRQADSMEDAL
                     AAARDLGYPVVLKTARPEVIHKSEVRGVRLNLADEPSVREAYEDLRRRLGPRVLVQET
                     AAPGVEWFLGGRQDPNFGPVLVTGLGGIYVEVFRETALRVGPVSSAQADRMLDECKAS
                     AILGGIRGRDPLDRRALVDMAVRASWLLHDFPEIRELDLNPVGVFPDGCRALDWRAVK
                     GPVP"
     gene            13628..14617
                     /locus_tag="PLM79_09405"
     CDS             13628..14617
                     /locus_tag="PLM79_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOV86567.1"
                     /translation="MMEKVPRLLMNAVLVLCCFLPSLGHAEGRVYSLKDYKDGAAFLE
                     SIPLPGGRDSRARRDLTALFSAYPDHCAGIELSEENRLYVVMNKGRRLVYEDGKGKSF
                     REKLEDPDLQDMLEQEYRPGPVEEDPPEDFDPGRIRVTAFFQAVYGETRDEVVGNCLE
                     TPFLGERVPFNVRGGARDALRKVDEELSALLRANPPLAKHVRPLGGSFSWRWIDGTRR
                     LSPHAFAAALDLNPARGAYWRWGTGTDPGEARRRYPREIVRAFEKHGFIWGGKWYHYD
                     LMHFEFRPELLFSHRISAEAPKPAPWAPRRQSRASNEQSRPGKETPGSWKIED"
     gene            14622..14990
                     /locus_tag="PLM79_09410"
     CDS             14622..14990
                     /locus_tag="PLM79_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOV86568.1"
                     /translation="MDIRTANTLLYCTRWKETVEFYRTVLKLPVCFSNEWFVEFMLND
                     SSRLSVADDSRASVKSGGGRGITVSLHVDDLEAVHGHLREAGCNPSPIKPVWGSPAFT
                     VLDPEGHRLEFWEKQNRRSS"
     gene            15606..16646
                     /locus_tag="PLM79_09415"
     CDS             15606..16646
                     /locus_tag="PLM79_09415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV86569.1"
                     /translation="MSVQQDPVEVEPGQSYEIDLFKPEDAPGITRLFRSVYGEEYPAK
                     HVYDPASIRRLFESGDNYPVVARTVGGDIVGHTALFRNAPNRCLYESGAGLVLPSYRN
                     RGINSALMRYAFEDAALRDRADMEALWGEPVCSHLHMQKTTSRLGLVEMGLEADLLPS
                     EAYGRERGTPGRTCALPGFRIYRSNPHPVYVPKAYRVHLAFLYSALDDCRRFLLSTEP
                     LPRSCRTEPRTEFLESTRVARVDLAAAGEDLDESMAALEAKLSPKEPAVIQVRLSLGL
                     PWVGRGVDVLRERGYFLGGVLPGWFEDDGLLLQKTAGSPAWDSIRLYTDRARAIRDMV
                     RADWVETGRSTS"
     gene            complement(16669..18726)
                     /locus_tag="PLM79_09420"
     CDS             complement(16669..18726)
                     /locus_tag="PLM79_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HOV86570.1"
                     /translation="MTRPGLEEITAGTVLEFFESKQVLCGVCLGVKNQRFTVLTEQNK
                     EMNLSQSRLIHWGGTFLDPGLGRDELVKRLGEMSALRKAFMTRIDVPELWSLLEGEGE
                     SYDARALAGFVFNSPVTADHAAALQRVLLEDRLFFQFKDGRFFARSREKVEQRRQEIE
                     REEQREAQLEEGSEWLHALWNRKPRPPLSADSEERFVEILKDFCLHGQESVHISFARE
                     LFKRANVPAQQNAAFRMLVRLGIWHEDENLYLLEQNLTPEFPPEIQQAGARIAARDFL
                     SRWDPSRRKDLRDLHTFTVDHSLTRDYDDALSLRTLEDGSYEVGIHIADAAEFVHKED
                     CLDLEAQERASSIYLPDGRIAMLPVELSEGVCSLKAHEDRFALSFLIRVDPEGTFLEH
                     EIVSSVIRVKEQLTYETVNERCREEPFLRILYELALRFRNQRIARGAILLPLPEIHVY
                     VDSAGMIRIHRYEKEIPGQIMVSEWMIAANYLAAAYLAERGIPTVFRGQGECRPENEL
                     VQSRHELFAVYRRRRLFSRAELDTEPRSHCSLALPCYTTITSPIRRYSDLISQRQLKQ
                     ALGGGEALYTREELQQILARLTATQSKIFYIQRKWTRYWLLKYIEQEDLQIREALVLD
                     QNDRYAHVVIPEFLLETSVPLPEKTRLQQGEMVRIRIDRVNPREDILRVQLAEPPSRP
                     HAE"
     gene            complement(18723..>19987)
                     /locus_tag="PLM79_09425"
     CDS             complement(18723..>19987)
                     /locus_tag="PLM79_09425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOV86571.1"
                     /translation="KVSVATVPPGGGTAGTAAKPAGSSPGTSGSSTPSSRATAPPLAP
                     CPNLVRMEKIQHWSGEDYTRVAVYTSGPVGFDEHAIPADPKNNQPGKIYVEVKDCVIS
                     PKMKSEIRIMDAFLQEVRAEQCDASLARVVLDTKPIGSYRIFTLSDPSRLIIDVRGGK
                     PKSAAPVVKQIPKATGPSAPSLARQLGLEVKRIVIDPGHGGKDKGAISPNGTYEKDIT
                     LAIAFQLKKGIEAKTGCEVILTRTKDRFLSLEERTAIANTSKADLFVSLHTNAHQDRS
                     HYGIETYFLNLSKDKESARVAAFENATSTKKISDLEAILHDLMLNTKLNESSRLASEV
                     HKNLVSGLKPRYTGIRDLGVKQAPFYVLLGAEMPSILIETAFLTNEREEKRLKDKSFQ
                     EALAVGIASGIESYIQHMKNYARSGEAS"
BASE COUNT         3698 a         6008 c         6452 g         3829 t
ORIGIN      
        1 ccccaacttg acggtgttgg aaaacctcga gctcggggct tattatcgaa cctcccggga
       61 ggtttccgcc gagctcaagc gggtctattc cctctttccg cggctcgagg agaggcagcg
      121 gcagaaagcg ggcactctca gcgggggaga gcagcagatg gtcgccctgg gacgggcgct
      181 catgagccgt ccgagactgc tgctcctgga cgaaccttcc ctgggactgg cacccctgat
      241 ggtcgaggag gtgctcgagg cggtccaaag gatgaaccgg gaaggagcga ccatcttgct
      301 catcgaacaa aacgccatgg cggccctccg ggtggcccac tacggatatg tgatcgagac
      361 gggaaggatt gtgctggaag gggaggcccg ggatctcctc gaggaccagc gagtccgcag
      421 ggcttacctg ggggaaggct gaagtttcgg ctcacaggaa gtaccatggc tgtgtcgttg
      481 taatgtttac gaatcaaggc aggggatccc catgaagcaa atgagcttct ttcagaaacc
      541 caccccggga aaagggggag ctccggcacc tctggccgag cgaatgcggc cccggacttt
      601 gggcgaattc gtgggacaag agcacctgct ggggccggga aagatcttgg atcgtctgct
      661 ccgcaaccgc cgaatgcagt ccctggtctt ctggggaccc cccggttccg gcaagaccac
      721 cctggcgaga attcttgcgg atcaatcgga cgctcacatg atcgccctct cggcggtcct
      781 ggcaggcacc aaggaaattc gggaggcggt cggggaagcc cgtgagcgtt ggaagcgcct
      841 cgggaagcgg acgctccttt tcatggacga ggtgcatcgg ctcaacaagg cccagcagga
      901 cagtctgctg ccccacgtcg aggacgggac cttctggttt ttgggagcca cgacggaaaa
      961 cccttccttc gaagtcattc gtccgctgct gtcccgaacc caggtgctgg tgctcgagcc
     1021 cctggatgag gaaagcctcc gggccattct gcgccgggcg atgacggata gggaacgggg
     1081 gctgggggcc tatcccgccg aaatcgcgcc cgaagcggaa gaatacctgg tttccgcggt
     1141 gggcggggat gcccgggtgc tgctcaatgt cctcgaagtg gcggtgacga ccacggcccc
     1201 gggaggcgac ggaattcgcc gtctggacct cgactcggtg cgggaggccg tccagcgccg
     1261 agcagtccac tacgacaagg gaggcgaaga acactacaat ctcatttcgg ctctccacaa
     1321 gagcctccgg gggagcgacc ccgacgcggc cctctactgg ctggcccgaa tgctccgggc
     1381 gggagaagac cctctttaca tcgctcgccg gatggttcga acggcatccg aggatatcgg
     1441 gctggcggac ccccgggctt tggaacaggc catggccgcg gtgcgttcct tcgaattcct
     1501 gggaagcccc gaaggagagc tggccttggc ccaggccgcc gtgtacctgg ccctggcgcc
     1561 caagagcaat gccctgtaca cggccttcaa gaaagcccgg gccgctgcgg aggagtccgg
     1621 cgacgccccg gtgcccctcc ccctgagaaa cgcccccacc gagctcatga aaaggcttgg
     1681 ctacggccgg gactaccttt atcctcacga tcaccccgaa ggatgggtcc ccgagaacta
     1741 tctgcccgac ggccttgagg acagggaatt cttccacccc actcgaaggg gctgggagaa
     1801 acgtttcggc gaagaactga accaacggcg gcagcgggtt cgccacaaga atagatcggc
     1861 caaggcccat gactgaagaa aatcggtccg ccggggaggg acaccccgag acgggcggaa
     1921 aggaaacccc atctccgaaa cccgctcggg aaaggaatgt cggtcccgag gccatcaagc
     1981 ccttccggct ggtgaagtat ctttccctca ccagcctcat ggtcatcctg gtgtgcacca
     2041 tgtttctctc ggggttcatt tctcagcggg ccaaaacgat cctggtccag aagagcgagc
     2101 aatatgccct gctcatcgcc gaaaacttga accaccaggt tttctaccaa tttaccgtgc
     2161 cgaccctcat caccgaggga gaaatccgcc ttagccgccc caaccagttc gaacggctgg
     2221 accgggtggt ccgaaacacc atccagggat ttagcgtcga gcaggtgaac atctatgacc
     2281 cggagcagat tctcagctac agcacggatc cccagcgggt gggaatgaaa ggggacatcg
     2341 gggacttgtt cgaacgggcg tggagggaag agaccgtttc agtactctcc ggggagggaa
     2401 gttctttcct ggggttcgaa tggcgggccg gggcccggaa gctgaccgcc tacctcccca
     2461 tgtgggagga gcggagcatg acccagatgc gggggaaggt cctgggggtg ttcgagatca
     2521 ccctggacgt gacggacgac tacaaggcca ttcaccggtt ccagtggatc atcgtcctca
     2581 gttttctcgg ttttgtggga attctcttcg tcacgattct gctcatcgcc gcccgtgcgg
     2641 agaagatcat cgccgcccgt gccgcagagc ggctaaagct cgaagaacag ctccaccaga
     2701 ccgagcggct ggcggccctg ggggaaatga ttgccggggt gtcccacgag atccgaaatc
     2761 cgctggggat catccgtacc acggcggagc tgctgcacgg aaggcttgag aacgatcggc
     2821 aaaagaggct ttcttccatc atcgtggaag agtccacgag gctgaacgat attctaacgg
     2881 aattcctgga ctttgcccga cccaaggccc tcagggtgca gtcgtgccgc ctcgaggaag
     2941 tcctggatcg aaacctcgag gtgatggaac ccgagtgcca gaggctgggc atccaggtcg
     3001 agcgggacta ccggacgggt ccctacggga tggaggccga cgcagacctg ctttaccggg
     3061 cgtttgtcaa cctgctggcc aatgctctcc aggccatgcc ccagggggga cggctccgcc
     3121 tcgaaacgtc cttcgccaac ggtcgggacg gaagtcccca catcgagttg aggatccagg
     3181 attccgggca cggaattccc gccgagttga aaaagaaaat cttcaatccc tttttcacca
     3241 cccgggagaa aggaacggga ttgggccttg ccattgtgcg cagcatcatt gacggacaca
     3301 acggcgacat ggaggtcgtg agcgaggaag gaaaaggaac aaccatgatt gtccgcctgc
     3361 ctctggtgca gcccaaccgg gaggcggcgg gagaggagag tcgatagagt cccatggaaa
     3421 cggttctcat tgtggatgat gagaggaact acctcctggt gctggaggac ctcctcatgg
     3481 aagagggcta cgaggtcctg acggcggaca gcggcaccag ggcccttgaa gtcgcccggg
     3541 agcatgagct cgatgccgtg atctcggaca tcaagatgcc gggcatggac ggcatggccc
     3601 tcctcgagtg gtttcacgac cacaaaccgg atctgcccgt catcatgatg accgcctacg
     3661 gttccatgga aaaggccatg gaagccaccc gccggggagc cttcgattac atcttcaaac
     3721 cgttcaagaa cgagcagctc aagttcaccg tccgcaaggc catcgaccat taccacctgc
     3781 tgtgccgaaa ccgcgatctc acccgggagc tccaggaacg gtaccagttc ggcaacatca
     3841 tcggcaagag catcgggatg cagcggatct accagctcat cgagaaagtg gctcccgcca
     3901 aggcgacggt cttgatcacc ggggaatcgg gaaccggcaa ggagctcatt gccaaggcca
     3961 tccacttcaa cagcccccgg cgggatcagc cgtttattac cgtcaactgc ggcgctctcc
     4021 ccgagactct cctcgagagc gagctcttcg ggcacgagcg aggggcgttc agcggcgcag
     4081 tggctcagag gaaggggcgg ttcgaactcg cccacgaggg gacgctcttc ctggacgaaa
     4141 tcagcgagat gtctccgccc ctccaggtca aactccttcg cgtcctccag gagatggagt
     4201 tcgagcgggt ggggggaagc cagagcctca aggtggatgt ccgcgtggtg gccgcctcca
     4261 accgaaacct caaggaagaa gtggcggcgg gtcggtttcg ctcggacctg ttctaccgcc
     4321 tcaacgtcgt gcacgtccag ctgcccccgt tgcgatccag gaaagacgac atccccctgc
     4381 tggtcactca ctttctcaag aagtatcccc ctcccggcgg acggcccatc cgcatggatt
     4441 ccgaagccat gcgcctgctg ctggaatacc cgtggcctgg aaacgtgcgg gagctcgaaa
     4501 acgtcatcga aagagcggtg attctatgca acggggaaca gatcctcgcc aaggacctcc
     4561 cgtcggaaat ccgagaaccg ttgcccggag gcaaaccgga accgggggga gagctccccg
     4621 gggtggggga gatcccctcc cgggccgagg aaacgggagg gatcgatgcc ggagatcgcc
     4681 tgccgtcctc gggcctgaaa ccacggcaga tcaaggccct cgagttcgtt cgggcccacg
     4741 ggttcatcac caaccgatac tattcccaga tcaacgacat ctccgagcgc caggccttgc
     4801 gggagctcaa cgaattggtg gacggggacc tgctccatcg cctgggcaag ggccgtgcct
     4861 gtcgatacgt gccggccggg gactccccgc ccgagacttc ctcctgatcc gggtcgggag
     4921 cccctcgatt gggccgattt cctcgatcct tcccacggca tgggtccttc tgcttccttt
     4981 tcctgttcgg ggtggttgcg aggatggcgg ttcaacccgt gctttcccat gccgtgcgga
     5041 atccatggcc ggggacgccg gccgtggaag ggaaggggac taccccgagg tccggctgca
     5101 cgacctcgaa ctcctggacc aggaaggcca gagggtgcgc tttgtgagcg acgtgatcgg
     5161 ggaccgggtt tccgtcgtca tccctttcta cacggcgtct cccaccacat accccgtcct
     5221 cgtgcatttc ctgggccgcg tgcaggatct tttgggggaa ggcccgggaa gagaaggggt
     5281 tctggtgtcc gtgaccgtga ccccggcaaa agacctgccg cctcgactcc gggaatacgc
     5341 aacacaaccc ggcgccgccc aaagcccggg tgggtctttc tcacgggagc cgggaggacc
     5401 tcacccgggt tttgcgggga acgggcctgc cggcttcccg gcgactcgag gatcacaacc
     5461 acatgccccg catgatcgtg gggagcctcg ggggccgatg gcgccggttc cacggctttc
     5521 cgtctcccag gcaaatcctc gaggaggcgg accgggccgc aaggaatcgg cacccttcgg
     5581 gggaaatccc tcccgggaac ggctcgatcg ccggaaacgg acccaggcct tgagaagggc
     5641 gcttcggggg gtgccttcgg acgatccggg agcaaccggc ttttgccttt ggacggaaca
     5701 agcgggttgg ctttgttctg acaatcgccg tttcacttca ccgtatggaa gtacggatcg
     5761 gtgcagaaga aaaatacagg atagtcccca ttttgcgaaa aaccggttcg gaggataatg
     5821 ggtgcggtca ggaaggcgct gaccgccggg ggcggccccg ggacccccag agatcggggc
     5881 cgcaggacga tcaacatccc agaaaaggag gggactccca tgagaaaaag tcgaatcatc
     5941 ctcggattcc tgctggtgac ggtcctgggt ttcggcaccg ttgccccggc ccagtcggcg
     6001 gacgacattg ccaagcatcc gtcgtgcaag tactgcggca tggaccggaa gcagttcgcc
     6061 cacagcagga tgctcatcga atacgatgat ggaacttccg tgggtctttg cagtatccac
     6121 tgtgcggcgg tggatctctc cctgaacatc gacaagactc cccaaagcat ccgggtggga
     6181 gactacaaaa ccaagaagct catcgaggcg gaagcggcta cctgggtcct ggggggcaac
     6241 aagccggggg tcatgtccaa gcgggccaag tgggcgttcg agggcaagga ggatgcccag
     6301 gggtacgtaa aggaaaacgg gggggagctg gtggtcctgg acgatgccat gaaggcttcc
     6361 tacgaagaca tgtattcgga taccaagatg attcgtgaga agagaaagat gatgaaaagc
     6421 aaacaggggg agcccaagaa ataggagcgg cgggctgcgg gtttgccgtc gctcccttcg
     6481 atggaggctt ccaaacggta gcgccgggtc ccgggcggtc tacaccgggc ggaaaggttg
     6541 agggctcagc accgatcggg gagagatccc ggatgcggaa ttgaagagcg tccgaacggc
     6601 gggagggcgg gtctgccgtt gtttccggac ggagcccgcc gggattccgg cccggaggcc
     6661 gtccaaatcc cgggacgagg gggatagggg tcacggggag cgtgtcacca tttcccggga
     6721 gctcttcgga tcccggtggg gggaagcagg atgaagaagt ggatatggat tttcctggtc
     6781 tggggactgt ggaccggggg tccggggtgt atggcggggg acacggaccc ggtaaagccc
     6841 accccccggg acaagtgccc cgtctgcggg atgtttgtgg ccaagtatcc ggacttcatt
     6901 gccgggatca agtatcccga cgggtcttac gccgtctttg acggggccaa ggatctcttc
     6961 aaatttttcc agaatgtgtc caagtaccat ccctcgagga aggtcgaaaa tattgtgtat
     7021 gtctgggtta cggactacta ctccctggag cccattgacg ggaaaaaggc ctactacgtg
     7081 gtgggaagcg acgtgtttgg gcccatggga agggagctga ttcctttcga gaaggaagag
     7141 gacgcccggg agttccagtc ggatcacgga ggcaccggga tcttttcctt cgacaaggtc
     7201 accccggagc tgatcaaggg cctggactga acatgttcga acgaaaatcc catcgtttct
     7261 ttgcctggtt gggggtaggt ttggtcctga tggggttcat ggggatccac gccgcctatc
     7321 gtcggaacgc ggataccccg atgctgcgcg gaaaggggca gatggttcgc cgactggccc
     7381 tcacggacct gtgccttttc accgacgccc ggtacgcccg gaacccggtt ctcgcggacc
     7441 tcaacacgcc cttccaggac tgcccgttgt ctctcgagca tttcccttcg gggtcccttc
     7501 tccccgtgcc gccgcacctg ggcgtgcacc cggcgcggtg atcccatgcg aatcctcgaa
     7561 aagcaaaaga atctcgtcga ctttaccctt tcgtccctcc tgaggaggaa gggcaaaaac
     7621 acggctctct tggtcgtgta tacgttcatg atcttcctgc tggcgtcggt catgttcttc
     7681 accgggtcca tcaagaagga agcggccctg gtgctcgaag gagcgccgga gatcaccgtg
     7741 cagcgccttg tggcgggcag gcacgacctc atcccggcag cctatctcga agccgttcgg
     7801 gaaatccggg gggtgcggtc ggccgccccc cggctctggg gctattatta cgatttcacc
     7861 agcggagcca acttcaccct catggtggac gagagcctca aggacagtcc cggggtcgtc
     7921 cacgtgggca gcggtgtgct ccggaccctc atgtcccccg agcacgggac gatccccatc
     7981 aaggcttccg acggttcctt tcatttcttc gagatccacc acgtcttccc cgccgaatcc
     8041 gacctggtgg cctcggacct ggtcctgatg tgcgaacagg actaccggaa ggtgttttcc
     8101 attcccgacg ggtttgccac ggacctggtc ctcgaggtgc gcaaccccaa ggaactcacc
     8161 accattgcgg ggaagatcac ccagctcctg cccgatacgc gccccatcct gcgggacgag
     8221 atcctgcgga cctacgaggc cgtcttcgac tggcggggcg gggttttggt cgtcattctt
     8281 tggggttccg tccttgcctt tgtcattttc gcctgggaca aggccacggg attgagcgcc
     8341 gaggaaaaaa gggaaatcgg ggttctcaag gccgtggggt gggagacttc cgatgtgctg
     8401 gtcatgaagt tctgggaagg gatgatcatc tccctatcct cgttcctgac gggggtcgtc
     8461 ctggcctacc tccacgtgtt cttcagttcc tcggtcctct tcgaaccggt cctcaagggc
     8521 tggtcggtac tttacccgga ctttcgactg actcccttca tcgacgggac ccagctggcg
     8581 ggcctcttct ttctcaccgt ggttccctac acggtcacga ccatcgttcc ttcgtggcgg
     8641 gcggccgtca tagagcccga ttccgtcatg cggtaggggc aggatgccgg aatgatcgaa
     8701 ctggcggatg tgaaaaaggt attcaacttg ggcaggccca acgagtttac ggccttggga
     8761 ggggtgagcc tttccatcga gctcgggaag gccacggcgt tcaaggggcc cagcggatcg
     8821 gggaagacca ccctcctcag cgtggtgggg tgcatggcga ggcccacggc cgggagggtt
     8881 cgcctcctgg accgggaaat caccagcctg cccgaacgat tcctgacgga aatccgccgc
     8941 aagacgttcg ggttcatctt ccagcagctc aatctcatcc gagggatcac cactctcgag
     9001 aatgtcatga tccccgccta tcccaacggg gagcggtatt cggagcttcg gcgccgggcc
     9061 ctggagctgc tggatctctt caacctgggc ggcaaggcgg aatcccgggt ggaatggctc
     9121 tcagggggtg aggcccagcg cgtggccatc gcccgtgccc tggtgaacca tccctccatc
     9181 ttggtggcgg acgagcccac ggctcacctg gacacccggc tctccatcga gttcatgggc
     9241 atcatgaagg acctcaagga ccgggggaaa accatcctca ttgccagcca cgacccggtg
     9301 gtcttcgagt cggaggggat cgacgtggtg gtggaaatga gagacggccg gatcgtgagc
     9361 taagggacgc ccatgctgct gcacccatcc atcctggcga tcctggtggg agccctcctc
     9421 gtaggcggca tgatcctttt ctcggtccac catgctgcaa ggatactccg atactgggac
     9481 ctggccggcg gcagcgaact ccagctcgaa ctcgagcgag ggacctacct ggtttcgacc
     9541 gtcatgagtt atgccctggg gttccaggtc ctgtccctct ttctgttcat cttcacggtg
     9601 gacgacatga gccccctgtt tgtgggggcc atgtgcgcgg cggggaccct gaacgccaac
     9661 gcctggggat atccgaccct gattctcaag gttgtcaatt cgctgtcggc gggcctgtgg
     9721 ctgattctga actatacgga caaccggggc tacgactacc ccctcatccg gatcaaatac
     9781 aagatgctgc tgtggatcgc accgggaatg atcctcgaga cgatcctgca gagcgcctat
     9841 ttcctcgatc tcaagcccga cgtcatcacc tcctgttgcg ggaccctctt tacctccgag
     9901 gcttcgggag cggcgggagg aatcctggtg ctgccccgcc aatgggtgga agcggctttc
     9961 tacgcggcca tgggagggac cctggcggcg ggtgtgttca cctgggccaa agggaagggg
    10021 gcctatcttt tctcggggtt cagctgcggg gcctttctac tgggggtcct gtccctcatc
    10081 tccttcattt ccctgtacta ctacgagctg ccgacccatc actgcccgtt ctgtgtgctg
    10141 cacccggaat atcactacgt cggttacggt ctttacggaa ccctcctggc gggggcggtg
    10201 acgggcatgg gaacgggagt gatcgagccg tttcgggagg tccccagcct ggccgagacc
    10261 ctccccggca ttcgtcgcgc cctggccctg gtttccgttc tcagctacgc gggattcgtg
    10321 gggatggtca cttaccaaat tctcttctcg aacctgaaga tgggcgactg atcggggaat
    10381 tgcccccggt tccgcgcccg gccgacggct cgtgcgtttg tcatttcccc tcgggggccc
    10441 cggcgagaac ccacgcctcg agtaggccga tttcctcggg agaaagccag ggaggacctc
    10501 ccttgggcat tcgcggagtg ctggtgcctt ttaccctccg gatgagctcg cttttggccg
    10561 gatctcccgc aacgatcacc gggcccccct tgcctccttt catcatgggg ccgtaagcgt
    10621 ccaggtgcag tccgcctgcc ggtcttctcc cgctgtgaca ggaagcgcag gattcccgca
    10681 aaatgcccgc aacggccgcg taggaaggag gaccttcggg tttctgggcc agtagaaccc
    10741 cggaccgcgt gcctgtctcg acgggaaagg ccgtgctgag cgtcgaaacg ctcttttcgt
    10801 ccagggagaa gccggggcag ggcaaggcag ccgccccgac cagcatgcaa aacagggcaa
    10861 aaaatccggt cttttttctc ataactccct ccttgaggtt gacgcttccg gagatcggcg
    10921 atcgagggac gattataggg gggagccggg ttttaggaaa gcctttccta accggaaccc
    10981 ggctcgatct ttgcctcgaa acgagcgatt tgttgccgtt ggacggtttc caaggaccgg
    11041 catgaagccc gccccaacgg cacggcaggc gagtcggtcc tgatccaagg ggcgacgccc
    11101 cgtccccgcc cgccgggagc gccgtcgggt ggagaacggc cggcaagaat cgcccgcccc
    11161 ggctttgcct ctgcccggaa cggccggttc ttgacaaaaa tcgtcttgcc gactaatcca
    11221 gatcgggttt cacaaatagg ttccgtcgta gttctcatcg ccgtatcgat tcatcaaagg
    11281 gctggggagg tcaagagtgg aagggttttt cgaggctcga agcgtcgccg tggtcggagt
    11341 ttccgccaat cccgggaacc tggcccaggc catcgtctac aacctgctcc agttctggtt
    11401 ccaggggctc gtttacctgg tgggtcccca tggagggagc tttgcgggcc acaagatcta
    11461 tccccgggtg cgggagcttc ccgaacctgt ggacctggcc gccattctcg tcccggcgtc
    11521 ggcggtcccc gaagtcttga gagattgcgg tgaaaaggga atcaagcgag cggtcctcat
    11581 gtcgggagga ttccgggaac tgggggacga acgccgtgcc ctggaggaag aggtggtcgg
    11641 cattctgggt gcctacggca tacgcatgat cggccccaac tgcctgggaa tcatgaaccg
    11701 gcgaagcggc ctggcggttc ccttcatgac ttttcaggcc accatgcccg tgggcggggt
    11761 ggccgtggtt tcccagagcg gcggggtggc ggccatgatg gtgaactgcc tcgaagcgga
    11821 aaacctgggg ttcagcaagt ttgccagtat cggcaacaag ctcgatgtga acgaagtgga
    11881 ccttctcgag tacctggttc gagacgaaga gacccgaatc gttttttgct acctcgaggg
    11941 aatcgccgac gggcggcgat tcatggaact cgcttcgggg tcctcgaagc ccgtggtgct
    12001 gcacaaatcc aaccggggtc gggccggatc cgtcatcgcc cgctcccatt cggcgtccct
    12061 ttccgccgac gaccgggtcc tggatgcggc tctgaggcaa tcgggggtcc tgcgtgtctt
    12121 tgaccagagg gaagccatga cggccatcaa ggggctggtg ctgcccccca tgaggggaaa
    12181 tcgcctggcc gtcgtgtccc ggtcgggggg acacgccgtc atggccgcgg atgcggccga
    12241 cgacctggga ttcgagcttc ccccttttcc cgagagcctc ttgcggacgg tacaggaaag
    12301 gtcccgggcc cgggtcatcc ggtttcacaa tcccctggac ctgggggacc tgttcgacat
    12361 gcccctgtac cacgccgttg ccgatgaaac cctggcccgg gacgacatcg acggcctcct
    12421 gttcatccac aattaccagg gggttttcga gcaggaagcc tcgaggcagg tcattgccgg
    12481 gcttgggtcc ctcatcgaaa agcaccgaaa accgcttgcc gtttgcgtgt tcaccacgaa
    12541 acccgagttg gaccgaaccc gagaaaaatc ccatttcccc atcttcaccg acccccggga
    12601 agccctcctg gccctggccg gaaatcggga gcatcatcgg gagcggcccg ctcccttctc
    12661 ccaccttcga ccccccgacg tcttcccgga tcgggtaaag aagcttctcg acgggctccc
    12721 cgaaggaccc atcggggcgg gtgtcctggg ggagatcctt tcggcctacg gcctgttttc
    12781 ggtgccctgg cggcaggcgg actcgatgga agatgccctg gccgcggccc gggacctggg
    12841 atacccggtg gtgttgaaga cggcccggcc cgaagtgatc cacaagagcg aagtccgggg
    12901 cgtgcggctg aacctcgccg acgagccctc ggtgcgggag gcctacgaag acctccgccg
    12961 tcgtctcggt ccccgggtcc tggtgcagga aacggccgcc cccggggtcg aatggttcct
    13021 ggggggacgg caggacccga acttcgggcc cgtcctggta acgggcctgg ggggcattta
    13081 cgtggaagtc tttcgggaga cggctctccg agtgggtccc gtctcgagtg cccaggcgga
    13141 ccggatgctc gatgagtgca aggcttcggc catcctcggg ggaattcggg ggcgggatcc
    13201 cctcgaccgt cgcgcccttg tggacatggc cgttcgggcg tcctggctcc tgcatgattt
    13261 tccggaaatc cgcgagctgg atctgaaccc cgtgggggtc ttccccgacg gctgccgggc
    13321 cttggattgg cgggccgtca agggccccgt gccctgagag gaccggaaaa aatctttccg
    13381 agcgccgcaa ccggaaccca gggagagcga tgtctccgat tgtatgggtg agcataaggc
    13441 ccgcccccgg gcctacgggc gaaccgtcgg ggcggggttc cgtccccgcc ctccgggaat
    13501 gccgttggat ggggaatgac catcgagaat cgcgcggttt taggcccaaa agacggggac
    13561 cgccgttccg ggttccccgg gtccgactgc tttggtctct cctccatgac ccggcaaacg
    13621 gaatgggatg atggaaaagg ttccccgcct cctgatgaac gcggtcctgg ttctttgctg
    13681 cttcctgccg agcctcggtc acgcagaagg ccgcgtctat tccctcaagg actacaaaga
    13741 cggggccgca tttcttgaat ccattcccct gcccggaggc cgggattcca gggcccggcg
    13801 ggatttgacc gccttgtttt cggcctaccc ggaccactgc gccggcatcg agctttccga
    13861 ggaaaaccgt ctttacgtgg taatgaacaa gggccggcgg ctggtttacg aggacgggaa
    13921 agggaaatcc ttccgggaaa agctcgaaga tccggacctc caggacatgc tcgaacagga
    13981 ataccggccg ggcccggtgg aggaagaccc cccggaagat ttcgacccgg gcaggatccg
    14041 ggtgacggcg ttcttccagg ccgtctacgg cgaaacccgg gacgaggtgg tgggaaactg
    14101 cctcgagacc cctttcctgg gggagagggt cccgttcaat gtccggggcg gtgcccggga
    14161 tgccttgcga aaagtcgacg aggaactttc ggcactcttg agggccaatc ctccattggc
    14221 aaaacacgtt cggcctttgg gggggagttt tagctggaga tggatcgacg ggacccggag
    14281 attgagcccc cacgcctttg ccgcggcact ggatctcaac ccggcccgtg gtgcctactg
    14341 gaggtgggga accgggaccg acccgggcga ggcccggagg agataccccc gggagatcgt
    14401 ccgtgccttc gaaaaacacg ggttcatctg gggcgggaag tggtatcact acgacctcat
    14461 gcacttcgaa ttccgacccg aactgctgtt ctcccatcgg atttccgccg aggccccgaa
    14521 accggctcca tgggctcccc ggcggcaaag ccgtgcctca aacgaacaga gtcggcccgg
    14581 aaaggaaacg ccgggctcgt ggaagatcga ggactgagcc catggatatt cgcacggcaa
    14641 acacccttct ctattgcacc cgctggaagg agaccgttga gttttaccgg acggtgctga
    14701 agctgcccgt ctgcttttcc aacgagtggt tcgtggaatt catgctgaac gattccagtc
    14761 ggttgagcgt tgcggacgac tcccgggcgt ccgtcaagag cggcggcggc agggggatca
    14821 ccgtgagcct gcacgtggac gacctggagg cggttcacgg gcacctgagg gaagcggggt
    14881 gcaacccttc gcccatcaaa cccgtttggg gttccccggc ctttaccgtc ctcgacccgg
    14941 aaggacatcg gctcgaattt tgggaaaaac aaaaccgtcg ttcttcatga aactgccgcc
    15001 ttctctccgt ggatgagctc ccatcgggcg gaaatcgact ccgggctggg caccccgggt
    15061 aaacgccgtg ccccgcctgc cgggcgccgg acccggaagt cgtccgtcac gcccgattcg
    15121 accttgcttt tggccggggc cggaaatcct cgtctgatca tgaaaccccc atcttcgtct
    15181 cgcccggagc gggcgagacg aagatggggg ctctgttgtc gcagacgctt tgcgcccgca
    15241 acaactaccg tctcatcggc agtgaagaca agtagaatcc cggactgcgc gatttggtcg
    15301 gtacgacccc cgtttggtgg gggattccgg gaagagggtg tatccccgcc ggatgcccgt
    15361 cgtcgcgggc atgatcgggt gatgctctcc caatcgctcg atcctggaga aaggcaaatg
    15421 ccccacaggg cgctgcggcg gacgcggcat ggccgcaccg ccaagcgttg ccttgtcttg
    15481 tgctccgaag cgggcccttt gctgatgccg tgtggggtgg gacggggcct ttccggcggg
    15541 gcggtctcca ttggttgtgg acccgtgcat cccatgattc caagaaacgg tatcgaggag
    15601 gggaaatgag cgttcagcag gacccggtcg aggttgagcc gggccaatca tatgaaatcg
    15661 atctcttcaa gccggaggac gccccgggga taacccggct cttccgcagc gtttacggag
    15721 aggagtatcc ggcaaagcac gtgtatgatc ccgcgagcat tcggaggctt ttcgaatccg
    15781 gagacaacta tcctgtggtg gcccgaacgg tcggggggga tatcgtgggc cacaccgcgc
    15841 tttttcggaa cgctcccaac cgttgtctct acgaatcggg tgcggggctc gttctgccca
    15901 gctaccgcaa ccggggcatc aacagtgcct tgatgcgcta tgccttcgag gatgcggccc
    15961 tgagagaccg cgcggacatg gaggccctgt ggggagaacc cgtctgcagc catcttcaca
    16021 tgcagaagac cacctcccgc ctggggctgg tggaaatggg cctcgaagcg gacctcctgc
    16081 cttccgaggc ttacgggcgg gaaagaggga cgccgggtcg cacctgcgct cttccgggtt
    16141 ttcggatcta ccggtcgaac ccgcaccccg tctatgtgcc caaggcgtac cgggtgcacc
    16201 tggcctttct ctactcggcc ctggacgact gccgcaggtt tcttctcagc acggagccgc
    16261 ttccccgaag ctgtaggacc gaaccccgga cggaatttct tgagagcacc cgggtggctc
    16321 gcgtggacct ggcggcggcg ggagaggacc tggacgagtc catggcggcc ctggaagcga
    16381 aactgagtcc caaggagcct gcggtcatcc aggttcggct gagcctgggg cttccgtggg
    16441 tggggcgcgg ggtcgatgtc ttgcgggaac gggggtattt cctgggggga gttctccccg
    16501 gctggtttga agacgacggg ctgctcctcc agaaaaccgc cgggtcccct gcctgggatt
    16561 ccatccggct gtacacggac cgggcccggg ccatccggga catggtccgt gcggattggg
    16621 tcgaaacggg aaggtcgact tcctgatcgg ggggtccgag tccggccctc actcggcgtg
    16681 aggcctcgag ggaggctcgg ccagctggac gcgcagaatg tcctcccggg gattcacccg
    16741 atcgatgcgg attcggacca tctccccctg ctgcagccga gtcttttcgg gaagggggac
    16801 gctggtctcg aggagaaatt cgggaatcac cacgtgggcg taccggtcgt tctggtccaa
    16861 caccagggct tcccggatct gcaggtcttc ctgctcgatg tacttgagga gccagtaccg
    16921 ggtccatttg cgctgaatgt agaagatctt ggactgggtc gccgtcagcc gggcgaggat
    16981 ctgctggagt tcttcccggg tataaagggc ctctcccccc cccagggcct gtttcagctg
    17041 gcgctgggag atgaggtccg agtacctgcg tatgggagac gtgatggtgg tgtagcaggg
    17101 caacgccagg ctgcagtggg atcgcggctc cgtgtccagc tccgccctgg aaaaaagcct
    17161 tcgccggcga tagacggcga agagttcatg cctgctctgg accagctcgt tttccggcct
    17221 gcattccccc tgtccccgga acaccgtggg gattccccgt tccgccaggt aagcggccgc
    17281 caggtaattg gcggcgatca tccactcgga caccatgatc tggccgggga tctccttctc
    17341 gtaccggtga atgcgaatca tccccgccga atccacgtag acgtggatct cgggcagggg
    17401 caagagaatg gctccccggg caatcctctg gttccggaac cggagggcca gttcgtaaag
    17461 gattcgcagg aagggctcct cccggcaccg ttcgttcacc gtttcgtagg tcagttgctc
    17521 cttgacccgg atgacgctcg agacaatttc gtgttcgagg aaggtcccct ccgggtctac
    17581 gcggatcagg aaactgaggg cgaagcggtc ctcgtgggcc ttcaggctgc agactccctc
    17641 ggagagctcc acgggaagca tggcgattct cccgtcgggc aaatagatgg agctggccct
    17701 ttcctgggct tcgaggtcga ggcagtcctc cttgtggacg aactcggccg cgtcggcgat
    17761 atgaattccc acctcgtagg agccgtcctc gagggttcga aggctgaggg cgtcgtcgta
    17821 gtctcgagtg agggagtggt ccacggtgaa ggtgtgcagg tcccggagat cttttctgcg
    17881 ggaggggtcc caccgggaaa gaaaatcccg ggctgcgatc cgggcaccgg cctgctgaat
    17941 ctccgggggg aactccgggg tgaggttctg ttcgaggaga tacaggttct cgtcttcgtg
    18001 ccagatgccc agccgaacca gcatgcggaa cgcggcgttt tgctgggccg ggacgttggc
    18061 ccgcttgaaa agctcccggg caaaagagat gtgaacggat tcctggccgt ggaggcaaaa
    18121 atccttgagg atttccacga acctctcttc ggaatctgcg gaaagcgggg ggcgcggctt
    18181 gcggttccat aaggcgtgaa gccactcgga gccttcctcg agctgcgctt cccgctgttc
    18241 ttcgcgttcg atctcctgtc gtctctgttc cactttctcc cgggagcggg cgaagaaccg
    18301 gccgtccttg aactggaaga agaggcggtc ttccaggagc actcgctgga gggcggccgc
    18361 atggtcggcg gtcacggggg aattgaacac gaagcctgcc agagcccggg catcgtagct
    18421 ttccccctcg ccttcaagga ggctccacag ctccgggacg tcgatccggg tcatgaaggc
    18481 cttgcgcaag gcggacattt cgcccagccg tttgaccagc tcgtcccggc ccagccccgg
    18541 gtccaggaac gttcctcccc agtggatgag gcggctttgg gagaggttca tttccttgtt
    18601 ctgttccgtg aggaccgtaa acctctggtt cttcacccca aggcacactc cgcacagcac
    18661 ctgcttggat tcaaaaaatt ccaggacggt gcccgcagtg atttcttcca ggccggggcg
    18721 ggtcatgagg cttctcccga ccgggcatag ttcttcatgt gctggatgta ggactcgatt
    18781 cccgacgcga tcccgacggc aagggcttcc tggaaggatt tgtctttgag tcgcttttcc
    18841 tcgcgttcat tggtgaggaa cgcggtttcg atgaggatac tgggcatttc tgctcctaaa
    18901 agcacgtaaa acggagcctg cttgactccc aggtcgcgga ttcccgtgta ccggggcttg
    18961 agacccgaca ccaggttttt gtggacctcg gaggccagcc gggaagattc gttgagcttg
    19021 gtgttgagca tgaggtcgtg gagaatggct tccaggtcgc tgatcttctt ggtcgaggtg
    19081 gcgttttcga aggccgccac ccgggccgat tccttgtcct tggaaaggtt gaggaaatag
    19141 gtctcgatgc cgtagtggct gcggtcctgg tgcgcattgg tgtggaggga gacgaacagg
    19201 tcggctttac tggtgttggc tatggccgtt cgttcctcga gggagagaaa ccggtccttg
    19261 gtgcgggtaa gaatgacctc gcaaccggtc ttggcctcga tccccttctt caactggaaa
    19321 gcgatggcca gggtgatgtc cttctcgtaa gttccgttgg ggctgatggc ccccttgtcc
    19381 ttcccgccgt gtcccggatc gatgacaatg cgtttgactt cgaggcccaa ctgccgggcg
    19441 aggctggggg ccgaaggacc cgtggccttg gggatctgtt tcaccaccgg ggctgcggac
    19501 ttgggctttc ctccccgcac atcgatgatg agacgggacg ggtcggaaag ggtgaaaatg
    19561 cgatagctcc cgatgggctt ggtgtccagc accactcggg ccagggaggc atcgcactgc
    19621 tccgcgcgaa cctcctggag gaaagcgtcc atgatccgga tttccgactt catctttgga
    19681 ctgatcacgc agtccttgac ctcgacgtag atcttgcccg gctggttgtt cttggggtcc
    19741 gcggggatgg catgttcatc gaatcccacc gggccgctgg tgtacaccgc cacccgggtg
    19801 tagtcctccc cggaccagtg ctggatcttt tccatgcgga ccaggttggg acagggggcg
    19861 agggggggcg cggtcgcgcg ggatgagggc gtcgaggacc cgctcgtccc cggggaagac
    19921 cctgccggtt tcgctgcagt cccggccgtt cccccgccgg ggggaaccgt ggcgacactc
    19981 actttct
//
LOCUS       DAOOVC010000060        19950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1831_length_19950_cov_7.272782, whole genome shotgun sequence.
ACCESSION   DAOOVC010000060 DAOOVC010000000
VERSION     DAOOVC010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19950
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1831_length_19950_cov_7.272782"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLM79_09430"
     CDS             <1..455
                     /locus_tag="PLM79_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOV86572.1"
                     /translation="LVKDLLPFLRARGYPERPGDYVAKAVRTLQPRARTLVEMADALR
                     FFLVDRVEPDPEAARKFLTRAMEQPFEKLISALDQLETFTEEGLERVFQQVVTELGIK
                     LGKIAQPVRVALTGTTVSPGLFEIIDVLGKETVLERLKSSLDLIRKTS"
     gene            610..2802
                     /locus_tag="PLM79_09435"
     CDS             610..2802
                     /locus_tag="PLM79_09435"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOV86573.1"
                     /translation="MSRLRDMPLRYRLGIPFLFLALFGTFSLVALAIISQDVLIGQEE
                     RERLHGYDRALDHNMSLQGRWAVSLASSFARNPEVVSALAERDRLRLIQLCYPAYLFM
                     KEDYGISQFNFHILPPRMFLRLQRLYEFGDELSYRQTILDALFTGKEVFGLEKGLTGY
                     GIRGVAPVFQEGNIIGTVEIGFSFGATLMQGMKKQFDIEVSILVPDESQTAFRSVLTT
                     FPRSFQRTDPLYVRVFRESRPEILLEHLQGQPYAILVRTVHDYKGNRVGLVEFCVNRT
                     ATLDLLTRYRWVMLGVGIVGMFLSVGGIYVISSYFTRPIGRMISFARSIAQGQPVKPI
                     KSYPSGEVGVLAEALDDMLASLDESRRKIRDYTDNLEAMVQERTRALRESEEKYRTLV
                     ENVPLVVYRTLGDGKTIFINHYIEVLMGVPVNRVLEDAAFWKEKVWEEDRPRIWPLMD
                     LCLEEGRAFKEEYRVLDREGNLVYVVDHALPVLDEEGKVETVDGFLMNVSDRHRLQNQ
                     IIQTEELRTLSQISARLAHEIRNPLVSAGGFARRLLRSLPPDEEVYRPKVEIIVREVA
                     RLEKILETTLSYLKPIEIAAEKSSLNQLIRSVIEDQGELLESRSIQVEFNLSPTLPLV
                     PIDTLLFRNALDGILSVLLNQCRFGTRLRIRTYLGQNALNLEMQFSGIQLSLDDMDHF
                     FYPFTSHESPPELLDLPLAKMIIHRHGGLIALEQKHPHELTLSITLPV"
     gene            3524..4693
                     /locus_tag="PLM79_09440"
     CDS             3524..4693
                     /locus_tag="PLM79_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOV86574.1"
                     /translation="MGEKGLIALLAVLSAFVPLSTDLYLPALPRMGEYFGVSSALTNL
                     TLVLFFISFSLCMLFWGPLSDKYGRRPTLLSGLAIYMLACAGCALSANIYMLLLCRVF
                     QAVGGSAASAVATAMIKDVYEGRRRESVLSIVQSMVVISPTVAPVLGAFLLRLMSWRG
                     LFWVLGLIGLLSFTGCLLLEETLRTRFQGTVPQALCRLGAVLRNPGFSSLLLVFSMVS
                     LASLAFIADSSYIYQDIFGLSAQTYSLYFALNAVGMLLGPILYLVLSRYYPRRSIVVA
                     CFGIMALAGLLVAAFGDAGPMFFALALLPATLMGSCVRPAGTFLLLEQHRGDAGAASS
                     LINCFGLILGSLGMIIVSLAGSDLLFVLGMINVFVGLTCAMAWLAICKRRPAVSA"
     gene            4716..4934
                     /locus_tag="PLM79_09445"
                     /pseudo
     CDS             4716..4934
                     /locus_tag="PLM79_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            5210..5713
                     /locus_tag="PLM79_09450"
     CDS             5210..5713
                     /locus_tag="PLM79_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86575.1"
                     /translation="MLGLERRKGREIADQAMSFTNVDHPAGRKLDELSAGERQRVLIA
                     RAVCQQSRIIVLDESTASLVPAHQVNIMGLMERLRSEEGITVVMVSHDLNLAALYADR
                     LLLMKEGHVVSLGVPAEVLTFETLERAYGCVLLVDENPLKKVPGLTLVPKKLLSVTCG
                     QREINSV"
     gene            6418..8496
                     /locus_tag="PLM79_09455"
     CDS             6418..8496
                     /locus_tag="PLM79_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV86576.1"
                     /translation="MEEVVVTATRTEKTLADVPASVSVITQEDMARQNIKTLDEALRL
                     TPGVFDKRTKGMMDATPSVNLRGFKGDQYTLVLVDGLPINDAYTGGVEWGTLPITNID
                     RIEVVRGPASALYGGNAMGGVINIITKTPEKLELEASGGYGSNNTYRYRLSGGDLLWD
                     RLSLQFGYEEEATDGFVTTPAYKTISPGSVAPSGGYAMDDPYGNHTRWVIGDTGDNGG
                     FHRAYNLKGGYKLSETGSVTFSMLSGRHEYDYGPPHTYMGRFGPGSDGGISFVPNDFI
                     SSTGIGRNDTDIYGVTFKEHFGPVTLRAHGGYVTSDDRYTLETGSGDADYRNSPGSLK
                     ITENNTWFGEISGDIPITQSHLLTMGVSYRRDSSDTDDFNVPFYRSYSNRGNSLFYSG
                     GMESNWGFFVQDEWRLLDPLTLYLGLRYDYWSLWDGASGVPGALKYYGSKTDSELSPK
                     FAAVWKALPDTTVRGSVGHAFRPPTLYDLYRSWQSGSIIYESNPDLKPETVWGYELGV
                     EQFLFSHKTRLSATGFWNDIENQVYYRSDGNLRQKINAGEVRTYGVELEAEQWITDWL
                     SVWGNYTFTHARVEENPTDPESEGKKLTGIPEHMFNLGADARYKWCKLSLIGRYSSKI
                     YSASDNSDIADGVYGSYEPAFFLDAKLTVSPIQRMDLYVSVQNILDKHYYEYYRSDGV
                     SVFGGITLRY"
     gene            8512..9567
                     /locus_tag="PLM79_09460"
     CDS             8512..9567
                     /locus_tag="PLM79_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86577.1"
                     /translation="MIAAYRVSIAAVLLLQPLCIIAPALGASQNEVVDALGRKVEVVT
                     PVRRIVALNSDAVEVLRILNVQDLIVGVFSQIDADPQFWGDLVNRRKVGSWREPNSEV
                     IVELEPDIVLAYAKTPSPEWEARMAARGIRVLRFELYRIDSLEREVRELGRVLDRKEE
                     AERFCSWHDLWLRRIREKVASVVPRPSVYVESYGDYVSAGPTTGGDQMCVDAGGRNIS
                     DVMAIPFPRVTPEWVVAQDPDVVVKAAVSNAKYGAAESGAALNRVRDRIMQRPAWNLI
                     KAVSSGRVHVLDSSIWVGPRAIIGLAYMIQWLHPGLMADLSPEALHKEYLETFQKLPY
                     KGVFVSDPVAGSDGKCP"
     gene            9564..10592
                     /locus_tag="PLM79_09465"
     CDS             9564..10592
                     /locus_tag="PLM79_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV86578.1"
                     /translation="MRSEAVSQGYIARNRQKKLLLVFAFLLLAMMAAVGLSVGASAVG
                     PLDVLESLFRDTGRANTIIWQLRMPRIVMAMLVGFGLGVAGTVFQAILKNPLASPFTL
                     GIGSGAGFGAVVVIIFMRGGLYPQLAAASAFVFSLLSAFFILGIAKMKHASSETMILT
                     GIAQMFLFSALSAFFQYMGTMEQVYEVTFWHFGSLSKAGWREIGFTSLMVLIPFPLLV
                     RRAWDFNLLATGDESALALGVDVERLRMWGVIAASLITAGCICFTGVIGFVGLVAPHI
                     VRMTMGSDHCTLIPASGLVGGLLVLAADILANTVWAPQVIPIGIMTSFIGVPFFFYLL
                     MKRTREYW"
     gene            10592..11359
                     /locus_tag="PLM79_09470"
     CDS             10592..11359
                     /locus_tag="PLM79_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86579.1"
                     /translation="MLEIVDLSFHYGSSTILRGIAAHVPKGRILCVAGPNGAGKTTLL
                     RCLTGIARPSGGQILIEGREVLRMTRRELATRVGYVPQHRPSGFPMTVFETVLMGRRP
                     HLTWRPSARDLELTADILRELNLEDLAMRDLDRLSGGQAQKVMLARALAQEPSFLVLD
                     EPTSSLDLCHQLEVMEMVQAVVGAGGMGAVIALHDLNLAARFADEIMLLKDGRVFARG
                     EPSVLIGSENIREVYRVDADVRMGNGRPWVQPLRCVR"
     gene            11428..15312
                     /gene="cobN"
                     /locus_tag="PLM79_09475"
     CDS             11428..15312
                     /gene="cobN"
                     /locus_tag="PLM79_09475"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004646.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HOV86580.1"
                     /translation="MKLRAIIAWPSVLGALVKTVKDLDYVDLKVWSMKELEEKREECL
                     RGLSDADILLFYVNTDYEAWKEVEPSLLEMSRAVPTLFIPAGLTPASLSTVSPKVAET
                     CLRYFSNGGMENLGNMVAFLASEVMGLSVEVRPPVELPREGIYHPDAPGCFLSLGDYL
                     DWYKPPGDMTAGIIFYRSYWVNGNCEVEKGLIRALESQGVGVVALFTESVGWSDNGSL
                     STAEAVSKFLMREDGTPLVDALVSLQFALLSGSRDGDGRLSVEEGVRLLDRLDVPVFH
                     PIVLYSRTEEEWRSELDSVNGSSIGWSVAMPEFEGMIEPLVAGALEREQDPDSGAIMD
                     RYGPIRERMKRIASRIKGWINLGKKPVSRRRAAFILHNNPCVSAEASVGGGAHLDTLE
                     SVAVILGRMEEDGYTVTDRPESGKELIETIMDRKALSEFRWTTVDEIVSKGGALAQLS
                     VDEYRRWFDRFPERIRQRMIETWGNPPGEEVNGAPAAMVHDGKILVTGVRYGNAVVCV
                     QPKRGCAGPRCDGKVCKILHDPEVPPPHQYLATYRYLEDHFGADVIIHVGTHGNLEFL
                     PGKPVGLSESCLPDLAIGNLPHLYIYNADNPPEGTIAKRRSYACLVDHMQTVLTGSGL
                     YDALEDLEKHLADYNRLKDTDPGRAHGLEHFILDSIQAANLERELGIQEGMSFDRVVE
                     KAHETLTLIRNTRIADGMHVLGQYPRGPRRADFISSIVRFNFGDGISSRRIVLDLMGI
                     DIDQAHENPAVIHPRLGKTFGEILDDADDCSLRLVAQVLADNGRSVIEIAGEMLDDRL
                     VNSEALSKLPALRERILEISRSIDDSMEIESLLAGFSGRYIRPGPSGLITRGRPDVLP
                     TGRNFYSIDPETIPTRPAWKVGVKLADALLERHRKESGTYPENLGMMWMASDIMWADG
                     EEMCQLMYLIGTRPIWTDNGRVKGFEVIPLEELNRPRVDVTVRVSGITRDCFPGAIDL
                     LDDAIRAVAALDEPLDLNAVRKHTLEQLESNDGSGMEEALRDATLRLFTSKPGTYGNG
                     VNLAVYASAWKEESDLSEIYIQWNGYGYGKGVYGKPSQRQLVRQLKSVDLTFNKVVSD
                     EYDLLGCCCYFATQGGMAAAAKHMSGREVKSYYGDTRDPEHVSVRDLADEIKRVVRTR
                     LLNPKWIEGMKRHGYKGAGDISKRVGRVYGWEATTGEVDDWVFDDIARTFLLDDENRR
                     FFLENNPWAIEEIGRRLLEAYERGLWEADDLVLESLKERYLETEAWIEEKIDGVTGDF
                     QGGAVDILGADDVADWNRRLEDVRRTIREEKA"
     gene            15345..16295
                     /locus_tag="PLM79_09480"
     CDS             15345..16295
                     /locus_tag="PLM79_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86581.1"
                     /translation="MAELLLRVLPVTFVALFGTELLVQLGMMKKLECLGRPLTRISCL
                     PPVTAMTFLAGIGSVVAANTMLAAYHNDGMIEDRELILSSLLNSVPIYLKELLTYHFP
                     VLFPLLGPWVGTIYFMTFRLAGFIKLVFIILGGRWLLGKKSRQRGEEASECAKTAAVG
                     GSERKSITVLIRSTFRSQLRLFARIASYYTVVTLIVLLCMELGLFKWMDSLMGPMARG
                     LGLPSSVVAPLSAYVVNPMVGLTSIAALLRSREITEYQAIVALLVAGFLMLPVIYARS
                     MLPKYVVIFGARLGSLIVTLSVGFTLLSRAIMLVAVVITG"
     gene            16380..18431
                     /locus_tag="PLM79_09485"
     CDS             16380..18431
                     /locus_tag="PLM79_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HOV86582.1"
                     /translation="MKKNALSPVFPFAAILGQEDMKLALLLNAVNPRIGGVLVRGEKG
                     TAKSTAVRALAALLPEITVLAGCLFECGPEEEERYCRRCMLCGGPRGAFTRKVRVVTL
                     PLNATEDRVAGGIDLDRTIHTGKRVVQPGLLARAHRGILYVDEVNLLDDHLVDVILDA
                     AASGENHIEREGISFTHPSRFELVGTMNPEEGELRPQLLDRFGLCVEIAAVVEREERV
                     AMMVAREEFDGDPEGFVFRFQAENRALADKVEKAQGKLSSVTLPDRLRNIIGDLCREA
                     FVAGHRADLVIEQAARAYAAFEGRTDVKVEDIMRVAPLALKHRRREAAPPPPPPKEHN
                     HPHDHDHDHEQSPDPTTDQEPTEESQGENSRLSQGHPQAESESDESSAPEPPRDPDAP
                     SNGPEQDILERVFEIGATFKVKRLATPRDRAFRRGSGRRSRTRVSGRQGRYVRSTEPR
                     GTKDVALDATLRAASPHQIRRKAGSDPAVVLRPQDIREKIRERRIGNFLLFVVDASGS
                     MGARGRMAATKGAIMSLLLDAYQKRDKVAMISFCRGEARLNLPPTSSIETAARLLAEM
                     PVGGRTPLSSALVKSSEVLRGHLVRDPTSKPIVLLMTDGRSNVSLGDGKPIDESLLLA
                     QKLGRDERVQFIVVDTEDQGLVRFGLAARLAGALSARYFRTNDLQADTLVDLVKGDSR
                     R"
     gene            18428..19501
                     /locus_tag="PLM79_09490"
     CDS             18428..19501
                     /locus_tag="PLM79_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86583.1"
                     /translation="MNDCTIYPFVAIVGQEQMKLALILNLVNPTLSGVLIRGEKGTAK
                     STAVRALAEILPEITVVKDCPFHLAPEDDTTLCRECFRTECREARLLQKPVEAVSRRI
                     SVVELPVGATEDRVVGTLDLEHALQKGEKRVEPGLLASVHRGILYIDEVNLLDDHVVD
                     VLLDSAAMGVNTIEREGVSFSHPARFVLVGTMNPEEGELRPQLLDRFGLCVRVEGITD
                     RDARVQVMQRRVAFDEDAARFVQSWEGESRDLVERIEAARALYPRVEIGLERLYAIAD
                     YCLEVGVDGHRGDIIMMKTAKALAAFNHRTEVRSEDVDGAAELALPHRVRRQPLMDVA
                     ESVEKVRRMKAPAQKTTGRITAL"
     gene            19708..19899
                     /locus_tag="PLM79_09495"
     CDS             19708..19899
                     /locus_tag="PLM79_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86584.1"
                     /translation="MNAAEALRSIRYDWLSYIQEKVRVDFDNARQAPVHFTHRARTHL
                     VSDVLGRFRNRDDSGLNGF"
BASE COUNT         3998 a         5864 c         6153 g         3935 t
ORIGIN      
        1 ccctggtgaa ggacctcctg ccgtttctcc gggcccgggg atatccggaa cgccccgggg
       61 actacgtggc aaaggccgtc cgcaccctgc agccgcgggc tcgaaccctg gtggaaatgg
      121 cggatgctct aaggttcttc ctggtggatc gagtggaacc ggaccccgag gcggccagga
      181 agttcctgac tcgggccatg gaacagccct tcgagaagct gatctcggcc ctggatcaac
      241 tggaaacctt tactgaagaa ggcctcgaaa gggtctttca gcaggtggtg acggaactgg
      301 ggatcaagtt gggcaagatc gcccagcccg ttcgagtggc cctgacggga accaccgtga
      361 gtcccggcct cttcgagatc atcgatgtgc tgggaaagga aaccgtgctc gagcggctga
      421 aaagctccct ggacctcatt cggaaaacct cgtagcacgg gacgccacaa ggatccggcc
      481 cgccgcctgc ccgccccggg tgcaggagga ggcggcctcc ggggaggggg ccccacgggg
      541 acccgcccgg ggaggagaaa tccatgtccc gacgcagatg ggacggaagc ccgaagggaa
      601 ggtgacccta tgagccgact acgcgatatg cctctgcgct atcgtctggg cattccgttc
      661 ctcttccttg cccttttcgg gaccttttcc ctggtcgccc tggccatcat cagccaggac
      721 gtcctcatcg ggcaggaaga acgcgaacga ctccacgggt atgatcgggc cctcgatcac
      781 aacatgtccc tccaaggccg gtgggccgtc agcctggcgt ccagtttcgc ccgaaacccc
      841 gaggtggtga gcgcccttgc cgaaagggac cgccttcgcc tcatccagct gtgctacccc
      901 gcctacctgt tcatgaaaga ggattacggc atcagccagt tcaacttcca tatccttcct
      961 ccccgcatgt ttcttcgact ccagcgactc tacgaattcg gcgacgaact gagctaccgc
     1021 cagaccatcc tggacgccct gttcacggga aaggaggtct tcggcctgga aaagggactc
     1081 acgggatacg gcattcgggg tgtggccccg gtgttccagg aaggcaacat catcggcacc
     1141 gttgaaatcg gtttcagctt cggcgcaact ttgatgcagg ggatgaagaa acagttcgac
     1201 atcgaggtct cgatcctggt tcccgacgag tctcaaacgg cctttcgaag tgttctcacc
     1261 acctttcctc gatccttcca acggaccgac cccctgtatg ttcgggtttt ccgggaatcc
     1321 aggcccgaga tcctcctcga gcatctccag ggacagccgt acgccatcct ggtgagaacg
     1381 gttcacgact acaagggcaa ccgggtgggc ctggtcgagt tctgcgtcaa ccggacggcc
     1441 accctcgatc tgctcacccg ctaccgttgg gtgatgctgg gcgtggggat cgtcgggatg
     1501 ttcctctccg tcggcggaat ttacgtcatc agcagctatt tcacccggcc catcgggaga
     1561 atgatctcct ttgcccggtc catcgcccag ggtcaaccgg tgaagcccat caaatcctac
     1621 ccctcggggg aggtgggggt gctggccgaa gccctggacg acatgctggc ttccctcgac
     1681 gaatcccggc gaaagatccg ggactacacg gacaacctgg aggccatggt gcaggagaga
     1741 acccgggcct tgagggaatc cgaagaaaaa taccgaaccc tggtggaaaa cgtgcccctg
     1801 gtcgtgtacc gaaccctggg agacggcaag accatcttca tcaaccacta catcgaggtc
     1861 ctcatggggg ttcccgtgaa ccgggtcctc gaggacgcag ccttctggaa ggagaaggtc
     1921 tgggaggaag accggccccg catatggccc ctcatggacc tttgcctgga agagggacgg
     1981 gccttcaaag aggaataccg ggtgttggac agggagggga acctggtcta cgtcgtggac
     2041 cacgcgctcc ccgtcctgga tgaagaggga aaggtggaaa ccgtggacgg gtttctcatg
     2101 aacgtcagcg accggcaccg gctgcaaaac cagatcatcc agaccgagga actgaggacc
     2161 ctcagccaga tttccgcccg cctggcccat gaaatccgca acccgctggt ttcggccggg
     2221 ggctttgccc gccgtcttct ccggagtctt ccgcccgacg aagaggtgta ccgccccaaa
     2281 gtggaaatta tcgtccgaga ggtggctcgc ctggagaaga tcctcgagac gaccctttcc
     2341 tacctgaaac ccatcgagat cgccgcggag aagtcctcat tgaatcagct catccgctcg
     2401 gtgatcgagg accagggaga gctcctggaa agcagatcca tccaggtgga gttcaacctt
     2461 tctccgaccc tgcccctggt tcccatcgat accctgctgt tccgaaacgc cctggacggc
     2521 atcctgagtg tgctgctgaa ccagtgccgg tttgggacaa gattgcgaat tcgtacctac
     2581 ctgggacaga acgccttgaa cctcgagatg cagttttcgg ggatccagct gtccctggac
     2641 gatatggacc atttcttcta ccccttcacc tcccacgaat cccccccgga actgctggac
     2701 ctgcctttgg ccaagatgat catccaccgg cacgggggac tgatcgccct cgagcagaag
     2761 catccccacg aactgaccct gagcatcacc ctgcccgtgt aaaatgggga atcgagatcc
     2821 tgcgttgagc gatcccgaga gcatcggggc gtcatgcccg cgacggcgcg catccagtgg
     2881 gtgggaacgc tccttccgga ttccctcctg cgcgggaatg acgaaacggg atgcgtgccg
     2941 gcaaaatcac tcaatccagg gatacgacaa aaacggcggc caagggcctt tgtgcactcc
     3001 atagtttgct tgaagcgctc cgccatcggg acgaggcgca ggaagaccca ttccgaccct
     3061 tgggaccgcc gcggttgccg ggccgggggt ggttcccgtc ctcacctacc gagaaatcgg
     3121 cacccatcgg tccttgacag aattcacaac acctgctacc cgaaagcctc gaccccggat
     3181 gagcgatttt tccccttgca tgggtgtgca taaagctcac ccctgggcct gcaggcgaac
     3241 cgtcctggac ggccggggcc ccgtccccgc ccgccgcggg agcgcccttg gatggggaat
     3301 gcccatcgag aatcgctcgg tttcggctgg aaaaaccggt ttcctctcac ttgacggccc
     3361 ttggttccag cagttccgtt catcccgctc ggagcactga gagacggccc ccccatccat
     3421 ggtaagcctg cgcaaaacgg aagaccaacg agccagatcc gacggagggg gtaagccgac
     3481 cccgttccgg atgggcgcac acggggtagt tggacagaga tacttgggcg agaaggggct
     3541 gatcgccctg ctcgcggtgc tgtcggcctt cgtccccctc tccaccgacc tgtatctccc
     3601 tgcgctgccc aggatggggg agtacttcgg ggtgtcctcc gccctcacca acctcaccct
     3661 ggtgctgttc ttcatctcct tcagtctatg catgctgttc tggggcccgc tgagcgacaa
     3721 gtacgggcgg cggccgacgc tcctcagcgg cttggcgatc tacatgctgg cctgcgccgg
     3781 gtgcgcgttg tccgcgaaca tttacatgct cctcctgtgc cgggtgttcc aggccgtcgg
     3841 aggaagcgcc gcctccgccg tggccacggc gatgatcaag gacgtgtacg aggggaggag
     3901 aagggagtcg gtgctctcca tcgtgcaatc gatggttgtg atatcgccga cggtggctcc
     3961 cgtgctgggc gcattccttc tccggctcat gtcctggcgc gggctgttct gggtgctggg
     4021 cctgatcggc ctgctatcct tcaccgggtg cctgctcctc gaggagaccc tgcggacgcg
     4081 tttccaaggc accgtgccgc aggccctgtg ccgcctgggc gcggtgctcc gcaatcccgg
     4141 gttcagctcc ctgctgctgg tcttctccat ggtgagcctt gcatccctgg ccttcatcgc
     4201 cgattcctcg tacatctacc aggatatctt cggcctctcg gctcaaacgt acagcctcta
     4261 cttcgccctg aacgccgtgg gaatgctcct gggaccgatt ctttacctgg tcctgtcgcg
     4321 atactacccc cgtcgctcga tcgtggttgc ttgcttcgga atcatggccc tggccggtct
     4381 gctggtcgcc gcgttcggcg acgccggtcc gatgttcttc gctctggcct tgttaccggc
     4441 caccctcatg gggtcctgcg ttcgcccggc cgggacgttc ctactgttgg agcagcacag
     4501 gggggacgcg ggggcggcct cctccctcat caactgcttc ggattgatac tggggagcct
     4561 gggcatgatc atcgtctccc tggccgggag cgacctgttg ttcgtgttgg ggatgatcaa
     4621 cgtgtttgtg ggattgacct gcgcaatggc ttggctggcc atatgcaagc gaagaccggc
     4681 cgtcagtgcc taaggaccct ctgttccttg gtgagcagat ccgcatcagc atggctggcc
     4741 ggggcagagc ctttgacaac atcttcatcg agcggctgtg gcgatcggcc aaatacgagg
     4801 aggtctacct cagagactat gagacggtta tggacgccgt cacgggaatc ggcagttacc
     4861 tggatctcta caaccatggg aggctgcacc agtctcttgg ctaccgcaca ccggcagagg
     4921 tctatgggag ctgattcact ggtgggctcc gccccccaag ccccccggag tttatcgctt
     4981 taggggaatc aaaaggggca ggggaaaaga tgggcggagt ccctgtcggg aagccccgcc
     5041 cctccgtcat ccccccacct cggcgctcag gttgctctcc agcagtggcc ttaccctccg
     5101 ggtgggcaga agaagcataa cacagcccgt tcagaaaggc tggtcggagg aggtgaaaaa
     5161 aagcttaggt tgacccgttt tttgtctaga ctttgggggg aagcttacgt tgcttggact
     5221 ggaaagacgc aagggtcggg aaatcgccga tcaggccatg tcctttacca acgtggatca
     5281 tccggccggg cgcaagctgg atgagctcag cgccggggag cgtcagcggg tgcttatcgc
     5341 ccgcgccgtc tgtcagcagt cccggatcat cgttctcgac gagtccaccg cgtccctcgt
     5401 tccggcccac caggtcaata tcatgggtct catggaaagg ctcagaagtg aggaagggat
     5461 aaccgtggtc atggtttccc atgatctcaa ccttgcggcc ttatacgcgg atcgtcttct
     5521 tctcatgaag gaggggcatg tcgtgagcct gggcgtgcct gccgaagtgc tcacttttga
     5581 aaccctggag agggcttatg gctgcgttct tctcgtggat gaaaacccgc tcaagaaggt
     5641 gcccggatta acgctggtgc cgaagaaact gctctccgtt acctgtgggc aaagagagat
     5701 caacagcgta taaccgtaca agcgatcggt ctgtttgttg gccaagtttt tggttgacag
     5761 cttatgcttt ttgctatagg agcccagaac acactaagct caaggctggc attccgcagc
     5821 aggtcccggt caatcgacgg ccctgaaggt cgttgtacat cttccggatc attttagggg
     5881 ttgcccgaag tcactttgca tgctccgggc ttgaatccca ggctcggccg gtgacgagcg
     5941 tgggcggttg tttggttctt aatgaagcca cgaaggctta tcacacgcga gatgcgtggg
     6001 aaggtcttcg tggctttttt gtttcgaggc ttgataacga atccggcccc aaaaagagga
     6061 agcagcagtc ctccggcaag agatatccgc tgcctcctcc ggatgaagcg cctcagggtg
     6121 aggcgggctt caatgccctg ataccattga agcccgccat ggtcaatctt tccacatcca
     6181 ccccgaggcg gatcgctcag ttccatcatc gaaaatctta gagggaaagg agacaacgac
     6241 aatggagggg gaaagatgaa gggggcactc agtctgttca tggcactgtg catattctgc
     6301 tcaattggtg ttgtttctcg gtcaggtgcg gaagaacaat gggaggaaac aactcgggcg
     6361 gcgtcggcaa ccatggaatc gggctccaaa aagtcgaccg ggcttccatc ggagcaaatg
     6421 gaggaagtgg tggtcaccgc caccagaaca gagaagaccc tggccgatgt tccggccagc
     6481 gtgtccgtga tcacgcagga agacatggcc aggcagaaca tcaagacact ggacgaggcg
     6541 cttcgcctga ctccaggcgt tttcgacaag agaaccaagg gaatgatgga tgcgacaccg
     6601 tccgtcaatc tgagaggctt caaaggagac caatacacat tggtgctcgt cgacggtctc
     6661 cccattaatg atgcctacac ggggggagtg gagtggggaa cgcttcccat caccaacata
     6721 gatcgcatcg aggtggtgcg cggtccggct tcggccctgt acggtggaaa cgccatgggc
     6781 ggggtcatca atatcatcac gaagaccccg gaaaagctgg aactcgaagc gagcggcggc
     6841 tacggctcca acaataccta tcgctaccgc ttgagcgggg gcgatctgtt gtgggatcgt
     6901 ctgagcctgc agttcggcta cgaggaagag gcaacggacg gctttgtcac gactcccgcg
     6961 tataagacta tctcccccgg gagcgtggcc ccgtccggag gctatgccat ggacgatcct
     7021 tacgggaatc acaccaggtg ggttatcggg gacactgggg acaacggggg attccatcga
     7081 gcctataacc tcaaaggcgg ctacaagctc tcggagaccg ggagtgtcac gttttcgatg
     7141 ctttcgggtc gtcatgaata cgactacggt ccgccccaca cttacatggg gaggtttgga
     7201 cccggcagcg acggcggcat cagcttcgtg cccaatgatt tcatctcatc gacaggtatt
     7261 gggcgcaacg atacggatat ctacggcgtc acgttcaaag agcatttcgg acctgtgacg
     7321 ctccgcgccc atggcggtta cgtgacaagc gacgatcgat ataccctgga gacggggtct
     7381 ggtgatgccg actatcgtaa ttccccgggc tcgctcaaaa tcaccgagaa taatacctgg
     7441 ttcggtgaaa tcagcggcga catccccata acccaatccc atcttctcac catgggcgtt
     7501 tcgtaccgga gggattcttc cgacaccgac gactttaacg ttccgttcta cagaagctat
     7561 tccaacaggg ggaacagcct tttctactcg ggagggatgg aatccaactg gggctttttc
     7621 gttcaggacg agtggcgcct cctggacccg ctcaccctct acctggggct caggtacgat
     7681 tactggagct tgtgggacgg tgcgtccggg gttcccggag ctctcaagta ctatggaagc
     7741 aagacggact ccgagctgag tccaaagttc gcggcagtgt ggaaggctct tcccgacacg
     7801 acggtgcggg gctccgtggg ccatgcgttt cgtccgccca ccctctacga tctttataga
     7861 tcatggcaaa gcggctccat catctacgaa agcaatcccg atctgaagcc ggaaaccgtc
     7921 tggggttacg aattgggtgt cgagcagttt ctgttcagcc ataagacgcg gctctccgcc
     7981 accggttttt ggaatgacat cgagaaccag gtttattacc gcagcgatgg gaatttgagg
     8041 cagaagatca atgccggcga agtgaggacc tacggcgtgg agctcgaagc cgaacaatgg
     8101 attacggact ggctctcagt ctggggcaac tacactttca cccatgccag ggtcgaagag
     8161 aatcccacgg atcccgaaag cgagggcaaa aaactcacgg gcatccccga gcacatgttc
     8221 aacctcggcg ctgatgccag gtacaagtgg tgcaagctga gtctgatcgg ccgctattct
     8281 agcaagatct acagcgcctc ggacaacagc gacatcgccg atggggtcta tggctcctac
     8341 gagccggctt tcttcctgga tgccaagctc accgtgagcc ctatccaacg tatggatctc
     8401 tatgtgtcgg tgcaaaacat tctcgataaa cactactacg aatactaccg gtccgatgga
     8461 gtctcggtat tcggtggaat cacgctgagg tactgagaag ggagaccctg gatgattgca
     8521 gcatatagag tctctatcgc ggcggtgctt cttcttcaac cgctctgtat catcgcacct
     8581 gctctcgggg cctcacagaa cgaagtggtc gatgctctgg ggaggaaagt cgaggtggtc
     8641 acgccggtgc gaaggatcgt ggcgctcaat tccgacgcgg tcgaggtcct tcgcatcctg
     8701 aacgtgcagg acctcatcgt gggagtcttc tcgcagatcg acgccgaccc acagttttgg
     8761 ggggacctgg tgaaccggcg caaggtgggc agctggcggg agcccaattc ggaagtcatc
     8821 gtggagctcg agcccgacat cgtactggcc tacgccaaga ctccctcgcc cgaatgggag
     8881 gctcgcatgg ccgcgcgggg gattcgagtg ctgcgcttcg agctctaccg gatcgattcc
     8941 ttggagcgcg aagtgaggga actgggccga gtgctcgacc ggaaggagga agcggagcgc
     9001 ttctgctctt ggcatgacct gtggctgaga cgcatccggg agaaggttgc gagtgtcgtt
     9061 cctcggcctt cggtatacgt ggagagctac ggcgattatg tttcggccgg gccgacgaca
     9121 gggggggacc agatgtgcgt cgacgcaggt ggtcgaaaca tctcggatgt gatggccatc
     9181 ccttttccgc gcgtcacgcc ggaatgggta gtggctcagg atccggatgt ggtcgtcaag
     9241 gcggccgtct ccaatgcaaa gtacggagca gcggaatcgg gcgcggctct caatcgagtt
     9301 cgggaccgca tcatgcaacg gccggcctgg aatctcatca aggctgtctc ttccggaagg
     9361 gtccacgttc ttgacagctc catctgggta gggcctcgag ccattatagg gcttgcctac
     9421 atgatccagt ggctgcatcc aggcctgatg gcggacctga gccccgaagc cttgcacaag
     9481 gagtacctcg agacatttca gaagctgccg tacaagggcg tcttcgtatc ggacccggtc
     9541 gccgggtctg acgggaaatg cccatgagat cggaagccgt ttcccaaggc tatatcgcca
     9601 ggaacaggca gaagaagctc cttcttgtgt ttgccttcct gctcctggcg atgatggctg
     9661 ccgtaggact atccgtcggc gcttccgccg tggggcctct tgatgtcctc gagtccttgt
     9721 tcagggacac cggtcgtgcc aataccatca tctggcagct gcggatgccg cgcatcgtca
     9781 tggccatgct cgtgggattc ggactgggtg tggccggcac ggtcttccag gcaatcctca
     9841 aaaaccctct cgcctcgccg ttcaccctcg gcatcgggtc gggagcgggc ttcggggccg
     9901 tcgtggtcat catcttcatg agaggagggc tctatccgca actggcggcg gcttcggctt
     9961 tcgtcttttc cctgctctcg gcgttcttca tcctcgggat tgccaaaatg aagcacgcgt
    10021 cctccgaaac catgatcctg acgggcatcg ctcagatgtt tctcttctcg gccctgagcg
    10081 cgttcttcca gtacatgggg accatggagc aggtctacga ggtcaccttc tggcatttcg
    10141 ggagcctgtc caaagccgga tggcgtgaaa tcggcttcac atccctcatg gtcctgattc
    10201 cgttccccct gctggtgcgt cgcgcatggg acttcaacct cctggccaca ggagacgagt
    10261 cggccctcgc cctgggagtc gatgttgagc ggttgcgcat gtggggcgta atcgcagcct
    10321 ccctcatcac cgcgggctgc atctgcttca ccggcgtcat cgggtttgta ggcctcgtgg
    10381 cgccccatat cgtccggatg accatgggga gcgaccattg caccctcata ccggcatccg
    10441 gcctggtggg gggattgctc gtgctggccg ccgacatcct agccaacacc gtatgggcgc
    10501 cccaggtcat ccccatcggc atcatgacct cctttatcgg cgtacccttc tttttctatc
    10561 tgctcatgaa acgtacccgg gagtattggt gatgctcgaa atcgtggatc tttctttcca
    10621 ttacgggagc tcgacgatcc tgcgggggat cgccgctcac gtgcccaagg ggcggatatt
    10681 gtgcgtcgct ggacccaatg gagcgggaaa gacgactctc ctccgttgcc tgaccggcat
    10741 cgccaggcct tcgggaggcc aaatcctcat cgagggccgt gaagtgctcc gcatgacacg
    10801 ccgggagttg gccacccgtg tgggctatgt tccgcagcac cgcccctccg gttttcccat
    10861 gacggttttc gagacggttc tcatgggcag aagaccgcac ctcacctggc gcccgtcggc
    10921 gcgggacctg gagcttaccg ccgatatcct tcgcgagctc aacctggagg atctggccat
    10981 gcgggatctg gatcggctga gcgggggcca ggcccagaag gtgatgctgg ccagggcact
    11041 ggcccaggag ccgtccttcc tggttctgga cgagcccacc agcagcctgg atctttgcca
    11101 ccagttggaa gtgatggaaa tggtccaggc cgtggttggc gccggcggca tgggtgcggt
    11161 gatcgccctg cacgacctca acctggccgc cagattcgct gacgaaatca tgctcctgaa
    11221 agacggcagg gtctttgccc ggggtgagcc ttccgtcctg atcgggagcg agaatatacg
    11281 cgaagtgtac agggtggacg ccgacgtgcg catggggaac ggccggccct gggtccagcc
    11341 cttgagatgc gtgcgctgag cgggatgggg ggcgaaagcc tgccaccgct gcatgcacac
    11401 gttgaaattc caggaaggag acgccggatg aagctgaggg cgatcattgc ctggccgagc
    11461 gtgctgggtg cgcttgtgaa gacggtcaag gacctcgact atgtggactt gaaggtctgg
    11521 tccatgaaag agctcgaaga aaagcgggag gagtgtctaa gagggctttc cgatgcagac
    11581 atcctccttt tctacgtcaa tacggattac gaagcctgga aggaggtcga gccatccctc
    11641 ctggagatga gccgcgccgt acccacgctg ttcatccccg cggggctgac cccggcgtca
    11701 ctgagcacgg tgagcccgaa ggtcgccgaa acctgtctgc gctacttcag caacggaggc
    11761 atggagaacc tcgggaacat ggtcgccttc ctcgcctcgg aggtcatggg cctttccgtc
    11821 gaagtccgac cgcccgtgga actgcccagg gaggggatct accatccgga tgcccccggc
    11881 tgtttcctct ctctcggcga ctacctcgat tggtacaaac cgccggggga tatgacggca
    11941 ggaatcatct tctatcgttc ttactgggtg aacggcaatt gcgaggtgga aaagggactg
    12001 atccgggcgc tggagagcca gggagtcggg gtagtggcgc tatttacaga aagcgtcgga
    12061 tggtcggaca acgggtcgct ttcgacggcc gaagcggttt caaaattcct catgagagag
    12121 gacggcacac ccctggtaga cgctttggtg agccttcaat tcgctctgct ctccggcagt
    12181 cgggacggag acggcagact gagcgtcgaa gaaggtgtca ggctgctcga taggctcgac
    12241 gtgccggtgt ttcaccccat cgtgctctat tcgaggaccg aggaggagtg gcgcagcgag
    12301 ctcgattcgg tcaatggcag ctccattggc tggtccgtgg ccatgcccga attcgagggc
    12361 atgatcgagc ctctcgtcgc gggggccctg gaaagagagc aggacccgga ctccggcgcg
    12421 atcatggacc ggtacggccc catccgggaa aggatgaaga ggatcgcctc caggatcaag
    12481 ggctggatca atctgggaaa aaagcccgtg agccgaagaa gggcggcctt cattctccac
    12541 aataatccat gcgtatcggc ggaggcttcg gtgggcggcg gagcgcacct ggatactctg
    12601 gaaagcgttg ccgtcatcct ggggaggatg gaggaggacg gctacaccgt tacggacagg
    12661 ccggagagcg gaaaggagct catagaaacc atcatggacc ggaaggccct atcggaattc
    12721 cggtggacca cggtggacga gatcgtcagc aagggcggcg ccctcgccca gctctccgtg
    12781 gacgagtacc gccggtggtt cgaccggttt cccgagcgca tcagacagcg gatgatcgaa
    12841 acctggggca atcctcccgg agaagaggtc aacggtgccc cggccgccat ggtccatgac
    12901 ggaaagatcc tcgtgacggg agtccggtac ggcaatgcgg tggtatgtgt tcagcccaag
    12961 cggggatgcg cgggaccgcg gtgcgacggg aaggtgtgca aaatcctcca tgatccggag
    13021 gtccctcctc ctcaccagta tctggccacc tatcggtacc tggaagacca cttcggcgcc
    13081 gacgtgatca tccatgtggg cacgcacggc aatctggaat tcctgcccgg aaagcccgta
    13141 ggcctttcag agagctgcct gccggacctg gccataggga acctccccca tctctacatc
    13201 tacaatgccg acaacccccc ggaagggacc attgccaaac gcaggagcta tgcatgcctg
    13261 gtggaccaca tgcagaccgt cctgaccggc agtgggctct atgatgccct ggaggacctc
    13321 gagaagcacc tggccgacta taataggcta aaggataccg accccggcag ggcccatggg
    13381 ctggagcact tcatcctgga cagcatccag gctgcgaacc tcgagcggga gcttggcatc
    13441 caggagggca tgtcttttga ccgcgttgtc gagaaggctc atgaaaccct caccctgatt
    13501 cgcaatacac ggattgcgga cggaatgcac gtcctgggtc aatatccccg gggaccaagg
    13561 cgagccgact tcatcagctc catcgtgcgg ttcaacttcg gagacgggat ctcgtcgcgg
    13621 agaatcgttc tggaccttat ggggatcgac atcgaccagg cccatgaaaa ccccgccgtc
    13681 atccatccgc ggcttggaaa gaccttcggg gagattctcg acgatgccga cgattgctcc
    13741 cttcggctgg tggcgcaggt gctggccgat aacggccgat ccgttatcga gatcgccggg
    13801 gagatgctcg atgaccggct cgtcaactcc gaagctctgt cgaagctccc cgctctcagg
    13861 gagaggatcc tcgagatatc ccgcagcatc gacgattcga tggaaatcga gagtcttctg
    13921 gccggctttt cggggcgcta cattcggccg ggtccctcgg gactcatcac caggggacgt
    13981 ccggacgtgt tgcccaccgg caggaatttt tactccatcg atcccgaaac catccccaca
    14041 aggcccgcct ggaaagtggg cgtaaagctt gccgatgcac ttttggaaag acacagaaag
    14101 gaatcgggca cgtatcccga gaacctgggc atgatgtgga tggcctcgga catcatgtgg
    14161 gcggacggcg aggagatgtg ccagctcatg tacctcatcg gtaccaggcc gatttggacc
    14221 gacaatgggc gcgtcaaggg attcgaggtc attcccctgg aagagctcaa caggcctcgg
    14281 gtggatgtga ccgtgcgcgt ttcgggcata acccgggatt gctttcccgg cgccatcgat
    14341 cttctggacg acgccattcg ggccgtggcg gccctcgatg agccccttga cctgaacgcc
    14401 gtgaggaagc atacgctgga gcagctcgaa tccaacgacg gttccggaat ggaagaggcg
    14461 ttgcgggacg ccactctcag gctcttcacg agcaagccgg gcacgtacgg caacggtgtg
    14521 aaccttgccg tctatgcctc cgcgtggaag gaagaatcgg atctttccga gatctacatt
    14581 caatggaacg gctacggtta cgggaaggga gtttacggaa agccctccca acgccaactc
    14641 gtgagacagc tcaagtccgt ggacctcacg ttcaacaagg tggtttccga cgaatacgac
    14701 ctgctgggat gctgctgcta tttcgcaacc cagggcggca tggcggccgc cgccaagcac
    14761 atgtcgggca gggaggtcaa gagctactac ggagatacga gggaccccga gcacgtttcg
    14821 gtgcgcgatc tggcagacga gatcaaaagg gtcgtccgga cccgattgct caatcccaag
    14881 tggatcgagg gaatgaagcg gcacggctac aagggagcag gagatatctc caaaagagtc
    14941 gggcgtgtct atggatggga agccaccaca ggcgaggttg acgactgggt tttcgacgat
    15001 attgccagga cgttccttct tgacgacgaa aaccgaaggt tttttctgga gaacaacccc
    15061 tgggcgatcg aggagatcgg acgaagactg ctggaggcct acgagcgagg attgtgggag
    15121 gcggacgact tggtcctcga atcgctcaag gaaaggtacc tcgagacgga agcctggatc
    15181 gaagagaaaa tagacggcgt gaccggagat tttcaaggag gcgccgtgga catcctcggc
    15241 gccgatgacg tggccgactg gaacagacgc ctcgaagacg tgaggcgcac catcagagaa
    15301 gagaaggcct gacggagaac ttcgtcctga atgtcgggaa ggagatggcc gagctcctcc
    15361 ttagggtgct ccccgtgacc ttcgtggcgc tattcggcac cgagctgctg gtgcagctcg
    15421 gtatgatgaa gaagctcgag tgcctgggaa ggcccctgac ccgaatctcc tgtcttcccc
    15481 ctgtaaccgc catgaccttc ctggcgggga taggctccgt cgtcgccgcc aataccatgt
    15541 tggcggcata ccacaacgac ggcatgatcg aggacaggga gctcatcctc tcgtcactct
    15601 tgaacagcgt tcccatttat ctcaaggaac ttctaaccta ccattttccc gtgctctttc
    15661 ctctcttggg accatgggtt gggacgatct atttcatgac cttccggctt gccgggttca
    15721 tcaaactggt tttcatcatc ctggggggaa gatggctgct cgggaagaag agcaggcaac
    15781 gcggggagga agcctcggaa tgtgccaaga cggccgcggt gggaggttcg gaacgaaagt
    15841 ccatcacggt gctcattcgg agcacgttca gaagccagct caggctcttt gcaaggatcg
    15901 cctcctacta caccgtcgtc acgctgatcg tcttgctgtg catggagctg ggtctattca
    15961 agtggatgga ctccctcatg ggaccgatgg cccgtggatt gggattgccc tcttccgtcg
    16021 tcgctccctt aagcgcatac gtcgtcaacc ccatggtcgg tctcacttcc atagccgccc
    16081 tgctccggag tcgcgagatc acggaatatc aggcgattgt cgcgctcctc gtagctgggt
    16141 ttctcatgct cccagtcatc tatgcacgct ccatgctccc caaatacgtg gtgatattcg
    16201 gcgcaaggct ggggtctctc attgtcaccc tgtccgtggg cttcaccctt ctgtccaggg
    16261 cgatcatgct ggttgctgtc gtgatcacgg gttgatcgag caggagaaag gaattttcga
    16321 atcggcaatg gacctcttta cctgggatct caacctgaat gcaagaaaga agaagagaga
    16381 tgaagaagaa tgcgctgtcg cccgtctttc cttttgcggc gatcctgggc caggaggaca
    16441 tgaagctggc tctgcttcta aatgcagtca acccgcgcat tggcggagtg ctggtgcgcg
    16501 gcgagaaggg gacggccaag tcgaccgccg taagggcact ggcggccctg ctccctgaaa
    16561 tcaccgtgtt agccggctgt ttgttcgaat gcgggcccga ggaggaagaa cgatattgcc
    16621 gtcggtgcat gctctgcggt gggccgcgag gagccttcac gcggaaggtg cgtgtcgtca
    16681 ccctgcccct caacgccacg gaggaccgcg ttgccggtgg catcgacctc gaccggacca
    16741 ttcacaccgg gaagcgagtc gttcagcccg gcctcctggc gcgggctcac cggggcatac
    16801 tctacgtaga cgaagtgaat ctcctcgatg atcacctggt ggacgtcatc ctggatgccg
    16861 ccgcctccgg ggaaaaccac atagagcgtg agggaatctc cttcacccat ccgtcccggt
    16921 ttgaactggt gggcaccatg aaccccgagg agggggagct tcggccgcaa ttgctcgatc
    16981 gcttcgggct gtgtgtggaa atcgcggctg ttgtggaacg ggaggaacgt gtggccatga
    17041 tggtcgcaag ggaggaattc gatggagatc cggaaggatt cgtcttcagg ttccaggcgg
    17101 agaaccgcgc ccttgcagac aaggtggaaa aagcgcaagg gaagttgagc agcgtcaccc
    17161 tgccggaccg tctgcgcaac atcatcggag atctgtgccg ggaagccttt gtggccgggc
    17221 accgggcgga cctggtgatc gagcaggcgg cccgcgccta tgcggctttc gagggacgaa
    17281 cggatgtgaa agtcgaggat atcatgcggg tggcacctct ggcgctaaag caccggcggc
    17341 gggaggccgc ccctcccccg ccgccgccga aggagcacaa tcacccgcat gatcacgatc
    17401 acgatcacga gcagtcaccc gaccccacca cagaccagga gccgaccgag gagagccagg
    17461 gcgagaattc ccggctctct caagggcatc cccaggccga gagcgagtcc gacgaatcct
    17521 ccgcacccga gccgccgaga gatccggacg ctccttccaa tggacccgag caagacatcc
    17581 tggagcgggt tttcgaaatt ggagccacct tcaaggtcaa acggctggcg acgcctagag
    17641 accgtgcttt caggcgcggc tcggggcggc gctcgcgcac ccgcgtgagc ggaaggcagg
    17701 ggcgttacgt gcgcagcacg gagcctcgtg ggacgaaaga cgtggccctg gacgctaccc
    17761 tcagagccgc ctctcctcac cagatccggc gtaaggcggg aagtgatccg gcagtagtcc
    17821 tgcgccccca ggacatccgg gagaagatcc gtgagcggcg gatcggcaac tttctcctct
    17881 tcgtggtgga cgccagcggc tccatggggg cgaggggaag aatggccgcc accaaagggg
    17941 ccatcatgtc ccttctcctc gacgcctacc agaaacggga caaggtggcc atgatctcct
    18001 tttgcagggg agaggcccgg ttgaacctgc ctcccacttc ttccatcgag acggccgcgc
    18061 ggcttctggc tgaaatgccc gttggaggcc gcaccccgct ttcctcggcc ctggtcaaaa
    18121 gcagcgaagt gctgcgcggt cacctggtgc gcgatcccac ctcaaaaccc attgtgctgc
    18181 tgatgaccga cggcaggtcc aacgtctccc tgggcgacgg caagcccatt gacgaatcct
    18241 tgctccttgc tcagaagctg ggccgggacg aacgggtcca gttcatcgtg gtggatacgg
    18301 aagaccaggg cctggtgcgc ttcggactgg cggccaggct cgccggggcg ctcagcgccc
    18361 gctacttccg aaccaatgac ttgcaggccg ataccctggt cgaccttgtg aagggagatt
    18421 ctcgtagatg aacgactgta ccatttatcc attcgttgcc atcgtgggtc aggaacagat
    18481 gaaactggcc ctcattctca atctggtcaa tccgaccctc tcgggcgtgc tgatccgggg
    18541 agaaaaggga accgccaagt ccacggccgt gcgcgccctg gcggaaatcc tgcccgaaat
    18601 cacggtcgtc aaggattgcc ctttccatct ggctcccgag gacgacacga ctctttgtcg
    18661 ggaatgcttc cgtacagaat gccgggaggc gcggctgctc cagaaaccgg ttgaagccgt
    18721 cagccggcgc atcagcgtcg tggagcttcc agtcggagcg accgaagacc gtgtggtggg
    18781 aacacttgat ttggagcacg ccctgcaaaa aggggaaaag cgtgtggagc cgggcctctt
    18841 ggcatcggtg cacaggggga tcctctatat agatgaagtg aacctcctcg acgaccacgt
    18901 ggtggacgtg ctgctcgact ctgccgccat gggagtgaac accatcgagc gcgaaggggt
    18961 ttccttctcc catcccgccc gcttcgttct ggtggggacc atgaacccag aagaaggaga
    19021 gctgcgcccg cagcttctcg accggttcgg cctgtgcgtg cgggtggaag gcatcacaga
    19081 ccgggacgcc cgcgtacagg tcatgcaacg gcgggtggcc ttcgacgagg acgccgcccg
    19141 tttcgtccag agctgggaag gcgaatcccg ggacctcgtc gagcggatcg aagcggctcg
    19201 ggcgctgtac ccccgggtgg agatcggtct cgaacggctg tacgccatcg ccgactattg
    19261 cctggaagtg ggcgtggacg gccacagggg agacatcatc atgatgaaga ccgccaaagc
    19321 ccttgcggca ttcaatcacc gaacggaagt gaggagcgag gacgtggacg gcgccgcgga
    19381 gctggctctg cctcatcggg tgcgcagaca gcccctgatg gacgtggcgg aaagtgttga
    19441 gaaagttcgc cggatgaagg cgccggctca aaagaccacc ggccgaatca cggccctgta
    19501 agaacgatcg tggctttctt cattagggaa ccacggtttc cgacctctcc ggattatcaa
    19561 gccaatgaag ccgggaaggt tttgacgggt gggaagactg cccggtcctc ccggcttttt
    19621 gtctcacggg taagaccggc ctgccgccca attcgaaacg gttttcactc atcttcttca
    19681 aaccacacac gataaggggg gtggtccatg aatgcagcag aggcattgag atccatacgg
    19741 tacgattggc tgagctacat ccaggaaaag gtgcgagtgg atttcgacaa tgctcgacag
    19801 gccccggttc atttcactca cagagcgcga acgcaccttg tcagtgatgt cttgggacgc
    19861 ttcaggaacc gggatgacag cggcctgaac gggttctagc aggatgttga attagcgggg
    19921 tgagctcaac ccctctttcc cgggtggatt
//
LOCUS       DAOOVC010000061        19665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1869_length_19665_cov_6.631311, whole genome shotgun sequence.
ACCESSION   DAOOVC010000061 DAOOVC010000000
VERSION     DAOOVC010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19665
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1869_length_19665_cov_6.631311"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLM79_09500"
     CDS             complement(<1..440)
                     /locus_tag="PLM79_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86585.1"
                     /translation="MMKVVGNQRGIAMVLAISLVALLSSLGVYLIWESGTAFRMTKAM
                     NRYESAFNLAEAALQLGLRCIRRSAPSPNFQQLNSGTVQTISEGVPSYIAAQSMGQGT
                     ITPRIDYLGYRTTPPPGWMLNWQGYSSLHGLYYRPGGQGSVPLPS"
     gene            complement(466..1491)
                     /locus_tag="PLM79_09505"
     CDS             complement(466..1491)
                     /locus_tag="PLM79_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86586.1"
                     /translation="MRSTLKALLQVRGMTLVEMMVAIGVTSVIMILSISIFMSQNKSY
                     VRSKNVKEVQESGQMMVEILKRDLMEAGWSVLPQMAFHFEDGGATGSDRIYVNDSSLI
                     DLSVATNRANMIGGDCAGGQRIVSGAGTSNVTIGRLDVNDDEGDGDSSGDPDFLAGVF
                     QFVISDATTNSIAKINTITSGPQLGLDRNLSGTHVAPAVYYCVDNGDAQCRPTGSTEQ
                     RVLRRSDRTTGGRQPMMENVVDMQVAYRDRNGNWYGAAGCAGTGVGAGLCAMDPFDPR
                     QVAIIRLTLVTRGADSVEGRIYDPAYCRPAVENRTAAASGSAECGYVYRTYSAMIQPR
                     NSGPLYQ"
     gene            complement(1513..1926)
                     /locus_tag="PLM79_09510"
     CDS             complement(1513..1926)
                     /locus_tag="PLM79_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOV86587.1"
                     /translation="MSLDAPDSRGFTLVEVMVALGAMAIAFTALWVLHLSSLSIDMKN
                     HSEIRAVFLANRQLEDLRSAAVLNFAGLADGTSTDNPEGAFTRTWQVTTVETWRKNVT
                     VTVTWPESVRTRGGGKTKLTRSLVLTSTLVDLELF"
     gene            complement(1916..2419)
                     /locus_tag="PLM79_09515"
     CDS             complement(1916..2419)
                     /locus_tag="PLM79_09515"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOV86588.1"
                     /translation="MRPVPFPRRNCRGFTLTEILVVIAVVIILTGAAYSGYSSWQRRE
                     QARAAAYELAGHLKEARFLAMEKNTGHTAVFVGNTYTVFSDPNNSMTLDAGERVLHQV
                     NVAQKNPGVTVSTSAGLSVHYNARGTISLRDSVNPNLLGATIAFSNAANTYTVTLSSL
                     GRIKIEP"
     gene            complement(2425..3213)
                     /locus_tag="PLM79_09520"
     CDS             complement(2425..3213)
                     /locus_tag="PLM79_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HOV86589.1"
                     /translation="MPDSPARRFLLCMVLLVLPWGLVSCAEQEPPVSGKKESQSQRLP
                     ARPVEGVESARQAGPAPAPGTAPSTEGPRGAAPTDSLAIEEVSIEPAFPVTGESLSVV
                     VKAKGLEGSFTDLTYSWRINGQVVQESTSDKLEKPVFRGDYVEVLVSPRVSAGSVKPV
                     TGCVVIANSAPNVQVQSQTMGSDGNYRAQLSALDPEKDPVTFSLKKGPPGMTIEATTG
                     TIHWSPGADVKGSFPVEVSAVDSQGAEAILSYQIQVRRETMGGT"
     gene            complement(3295..3828)
                     /locus_tag="PLM79_09525"
     CDS             complement(3295..3828)
                     /locus_tag="PLM79_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV86590.1"
                     /translation="MTRMRDRSQHAQRPGEGSISLQRGFSLVELMVVVAIAAILASVA
                     MPAYVNYINRARQSEAITALMNAKMDQEVFWEERAVGTLHRYAGTIGCLPSFVNSANT
                     SCLRDCSSCPQTSGVVGSYTVQVQSASTNSYQIIAFRKIYAAAPTDVVRISSTLDVPV
                     VVNENALKFSIFRLLFQ"
     gene            complement(4276..5943)
                     /locus_tag="PLM79_09530"
     CDS             complement(4276..5943)
                     /locus_tag="PLM79_09530"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86591.1"
                     /translation="MRPAESDSFDNGGIPLGDPLREQRELQILFDITSALHASPVLKE
                     VLQRALMAILRTLRFKMGAIYLFKEGLDEHGLLELAAHHGFSSSLVECIRSFSLTHEV
                     LDPGKRPQPVRWFPVSKLIFPRLRERMIEERVTEIICIPLMTQKRVLGLLYVTNDGVL
                     PLKPDRNEFLTTIGHQLGVAIDNAQLFDSVQRAKSELEISFDAIQHSIFVIDERWRIV
                     RVNRTCEEVYGGSRNLIGKKYSSVLYGTEEPPRDCPILMCLRTHERIQREGAHFRWGG
                     YYHYFASPVINLEGVLERVVYYEKDATEARKLEQRLQQSEKLKALGTLAAGVAHEIRN
                     PLATINFNAQMLRREIRLDSAQEQMFGDMVHEIRKIDRIVQQVLHFARPKEPQFLPNQ
                     LNDIVRYCYDLSKVYLRKAHVEISLRLAEVLPSLVMDHNQISQVIMNLVINAIEAMPQ
                     GGKLFLNTGRRDDPPALTLEIRDTGHGILEEDRSRIFDPFFTRKPEGTGLGLSISRQI
                     LEKHGAEIEVESVPGEGTVFRIIFPWSLVDGSGVKGFSKTPPLAPVP"
     gene            complement(6059..6985)
                     /locus_tag="PLM79_09535"
     CDS             complement(6059..6985)
                     /locus_tag="PLM79_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOV86592.1"
                     /translation="MERPTRLRRSILSVPSNREKMVLKAFALPVDVIMLDLEDSVPVQ
                     EKDEARSRVISILKEKDWGNKVRCYRINGMDTPFAYRDIVDVVEAAGDRIDVVVIPKV
                     NDAAEVKAIDYLLTQIELRMGFKVPIGLEASIETALGMLKVEEIAFSSPRLESLVFGV
                     ADYGASVTMMSKGISGHGDREEFYPGHRWHFPLSRMVMAAKAAGLEAIDAPYGDYKDP
                     EGLRRSCVLSAALGYDGKWAIHPDQVGIINDVYTPSAEDVERSRKIVEAYGKAQQEGC
                     GSLAIEGKMVDAASIRVAKMVCAQADAIARRS"
     gene            complement(7085..9847)
                     /gene="ppdK"
                     /locus_tag="PLM79_09540"
     CDS             complement(7085..9847)
                     /gene="ppdK"
                     /locus_tag="PLM79_09540"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOV86593.1"
                     /translation="MMAPKYVYFFEAGKADGNAQMKLLLGGKGANLAEMANLGIPVPP
                     GFTITTEVCSYYYKHNGTYPPELKAEVEEALHRLEQIMGREFGNPENPLLVSVRSGAA
                     SSMPGMMDTVLNLGLNDHTVVGLIQQTGDERFAYDCYRRFIAMYGDVVLGLKPDSKDE
                     KDPFDEILEEMKKRRNVEFDNQLPPHDLKELVERYKALIRERKKLEFRQDPMDQLWGA
                     ITAVFGSWNNPRAIAYRELNEIPEDMGTAVNVQAMVFGNMGDDCATGVAFTRNPATGE
                     NVFYGEYLVNAQGEDVVAGIRTPQPINNVQKAENHQTSLEEYMPEIYKELDEIRVKLD
                     RHYRDMQDIEFTIEKGKLWMLQTRSGKRTGFASFKIAVDMVRQGILDKEEALMRVTPD
                     QINQLLRPTFDPKAKEEAVRDGAFLARGLNAGPGAATGRVVFNAADAEEWARNGEVVI
                     LVRWETSPEDIRGMHASRGILTARGGMTSHAALVARQMGKVCVVGCNAIEIDYQGRRM
                     KAGDRFIREGQWLSLDGTTGEVFAGQIPTRPSEILRVIIDGSLSPRRSQVYQDFIALL
                     GWADEFRKLEVWANADQPNQAEEAIAFGAEGIGLARTEHMFFQGNRIDAVREMILAAD
                     AAGREQALAKLLPMQKEDFKGLFRVMKDAPVTIRTLDPPLHEFLPHEEKDIQKLATKM
                     GVSFEQIHSKVQSLHEANPMLGHRGCRLGIVYPEITAMQVRAIMEAACEVKKEGLDVH
                     PEIMIPLVGHVNELKHQREVADRVAKEVMKQYEVKVEYRVGTMIEVPRGAMTADEIAH
                     VAEFFSFGTNDLTQTVFGISRDDAGKFLPPYLTARIWEHDPFHRLDQVGVGGLMEIAV
                     AKGRKTRPNLKIGICGEHGGEASSIDFCHRIGLDYVSCSPYRVPAAQLAAAQSAIRDR
                     RAQQKK"
     gene            complement(10272..11156)
                     /locus_tag="PLM79_09545"
     CDS             complement(10272..11156)
                     /locus_tag="PLM79_09545"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOV86594.1"
                     /translation="MKLIGENLNIMNRKYGKALKEKDAKPLQELAVQEAEAGMDWIDL
                     NIGPAGKIGEELMSWLVPVIHEVVDLPLALDTSNIKAIEAGLKVHKKGRALINSIMVR
                     PERMSALLPLAAQYDADFVGLLWGPEGMPRDENERGALCSELVFSATTDHGIAPERIY
                     VDPIQTPVNVQQNQIMSVLEFMKMFPDIAPGCQSTIGLSNVSNGPPDHLRPILNRVML
                     IIWKRHGMASAIVDTFDKELHEIARGKRPELEALVHKVEDGVPVDLSSLSREETEYVK
                     TARVILGHSLYSDSWLEI"
     gene            complement(11374..12726)
                     /gene="acsC"
                     /locus_tag="PLM79_09550"
     CDS             complement(11374..12726)
                     /gene="acsC"
                     /locus_tag="PLM79_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HOV86595.1"
                     /translation="MGLTGIQIFKLLPKTNCGECGVPTCLAFAMNLAAGKAELDKCPY
                     VSEEAKEKLAAESAPPIRPLTVGTGECALKIGGETVLFRHEKTFVNPPGFAVLVTTDE
                     DAGSVDGKMERFKKCRYERVGLTLRAELFAVKDTSKDASRFTALAKKVSDGTGASLIL
                     MSEDVEVMKAAVSELKDKKPLLYAATPGNAEALGSLAKEAACPLAVKGDGTLDSVIGL
                     TEKLSQMGLKDLVIDTGTRDLKKAFEEQILIRRAALKDRFRPLGFPTMILANEMADNL
                     TEESLVAATFIAKYGAILVLSDFQPWSLFPLFLSRLNIYTDPQRPMTAEQGIYPINNP
                     GEDSPVLVTCNFSLTYFIVSGEIEASRVPSWLCVQDTEGLSVMTAWAAGKFSGETVGS
                     FINKCGIKEKVKHTTIVIPGYAAGISGELEEELGGWNVVVGPREASQIPKFLKVWKAA
                     "
     gene            complement(12776..14989)
                     /gene="acsB"
                     /locus_tag="PLM79_09555"
     CDS             complement(12776..14989)
                     /gene="acsB"
                     /locus_tag="PLM79_09555"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HOV86596.1"
                     /translation="MSRLVAFAAIQGAYNIVAKTEGSFRRAMDKYGPNQPLAFPNTAY
                     YLPVIYSILGIKVEKLADAEIVLKRCKELLPPHIKGKIHVPYLGHTLDAGMAAILAEE
                     IGEAIRYVEDPGFYLQGEDCDIENGKLWLGAADDVIMRKRGVEFVDGSAPGFAAIVGA
                     APTPEIAKDIVEDYQRKNLYIFLCANQNGTTCAQQLVQAGVQIGWGTRIVPFGPDISA
                     AVFALGFANRAAMAFGGVQPGDYRTVLKYNKDRIFAFVNALGEVNAEWAANAAGAINW
                     GFPTIADTDIPEILPTGICTYEHVVANVPHDHMTAKSIEVRGLKVTVTEVPVPVSYGP
                     AFEGERVRKDDVYLECGGGKTVCCELVQIAEMDAVEDGRVEVVGPDIKDVKPGSRLPL
                     AVVVEVAGRKMQADYEPILERQIHHLINYAQGIMHIGQRDIAWYRISKQAVEKGFTLE
                     HLGKILHAKYHQDFGAIFDKCQVKIYTEEAKVLETLPLAQHAYKIRDDRVEGMTDETT
                     DIYYSCTLCQSFAPTHVCVISPERTGLCGAYNWLDCKASYEINPTGPNQPIEKGETLD
                     SRYGNWKGVNDFVYKASRQSISSYNFYSLVYDPMTTCGCCECIAAVLPLCNGVMTVNR
                     DYNGMTPCGMKFTTLAGTIGGGNVTPGFVGHSKYNITQRKYIAGDGGFQRLVWMPKML
                     KEELRDRLIKRGEEIGVPGFIDMIATEEQGVTEEEIMAFLDSVGHPALSMESILG"
     gene            complement(15101..17056)
                     /gene="cooS"
                     /locus_tag="PLM79_09560"
     CDS             complement(15101..17056)
                     /gene="cooS"
                     /locus_tag="PLM79_09560"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HOV86597.1"
                     /translation="MSSEEKKTKQLNFRDVSTCEATIQMLQKAATDGVETAFSRAADM
                     KPCPIGADSACCKHCFMGPCRLVSKDPYSKVGVCGATIDTIQARNFARVVASGGSAHT
                     DHGMGILDVFREVVKGNIQEFQIKDEQKLRDVASSIGIAVAGRSTKEIAVDLYNELEK
                     TYTQVEGEIPFVKRVPPKTLELWRKHGVVPRGAMREIMEIMHRTHMGVDQDYENIVKQ
                     CSRTALADGWGGSMVATELSDILFGTPKPLVAGVNMGYLKEDHVNIIIHGHEPLLFEA
                     MIASANDPANLEKAKAAGAKGIQLLGMCCSGAEVLGRHGVPHAGNFLSTEAVIATGAV
                     DAMAVDVQCIMQALSSMSECYGTKFFTTNPRAKIEGAAHIEFNHHTPRESTEKIIAMA
                     LDRFKNRPAKIVIPQRRDLGVHGFSHEYVNYMLGGTFRGSYTPLNENIINGRIRGVAG
                     VVGCNNPRVKQDYAHVELVKELIKNNVLVVQTGCSQIALAKAGLMKPDAAVLAGEGLQ
                     EVCETVGMPPVLSLGSCVDNSRILIACAEMVRVGGLGNSIADLPVAGAAPEYYSEKAI
                     CIGQYFVASGVYTVFGVTFPSVEGTKFHQLLFEGLEELGFGKWGFAKDPIEIAHMMIR
                     HIDKKREALGIMGERERKLFSMEDRRA"
     gene            complement(17137..18081)
                     /locus_tag="PLM79_09565"
     CDS             complement(17137..18081)
                     /locus_tag="PLM79_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HOV86598.1"
                     /translation="MAFQPSKQVYSGKIREVTLGLGNKAVTVGGRTAYSFHTFEGQMP
                     HRPRVAMEVWDKDPSSEWSAVAIEPFKEVLGDPALWATKCVNEYGADLIVVQTKSADP
                     NGDNRPAADVAATVKKIVEAVDVPVVVWGVANHEKDVEVMRLVTEQSGGRHLAVAPVE
                     EGDYKQIGAACLGYGHTVVASSPIDVNLAKQLNILLGNLGVQGTEIVIDPTTGGLGYG
                     LEYTYSVLERIQQAALTQEDEKLQQPVIVNVANESWKAKEANLSLEEAPELGDPQKRG
                     VLMETITAVSLLLAGADIVVLRHPDSVQLVKKYIQTMV"
     gene            complement(18686..19207)
                     /locus_tag="PLM79_09570"
     CDS             complement(18686..19207)
                     /locus_tag="PLM79_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86599.1"
                     /translation="MMARIIYCHPAFTKYDFHVFTDLDFWDSRRILKDVAWVKRNFGG
                     LTCGDEFPTQVVLAVHDRLRIRSILRRLRKAVVSPPRHVIVRFMVFHGVFEFDPRKYY
                     PLHWSADQMIRFTFMRLPLHQGALCNPYQTVRLSWAGSNIRVERVQRELRVDSVVRTQ
                     GDARRALHVPSCF"
     gene            complement(19244..19591)
                     /locus_tag="PLM79_09575"
     CDS             complement(19244..19591)
                     /locus_tag="PLM79_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86600.1"
                     /translation="MIEVLESEPFFATVQREMFYVGFLHSREAWFPLCVVSDPGQKGR
                     LDTLCVAPSHALMDELVQSLVPRIPQVRESFIQYLGVGEIRELLSRYGLEKIAWVSFG
                     AEPPGCPCGCGCG"
BASE COUNT         3902 a         6082 c         5357 g         4324 t
ORIGIN      
        1 gaaggaagag ggacggaacc ttgccccccc ggtcgatagt agaggccgtg aagggaggaa
       61 taaccctgcc agttcagcat ccagccgggg gggggcgtgg tcctgtaccc caggtagtca
      121 atccgggggg taatggtccc ctgccccatg gattgggcgg cgatatagct gggcacgccc
      181 tccgagatgg tctggaccgt accgctgttt agctgctgaa agttgggaga aggggccgac
      241 cgtcgaatgc agcgcaatcc caactggagg gccgcctccg caaggttgaa ggcggattca
      301 taccgattca ttgccttggt catccgaaag gcggtcccgc tctcccaaat gagatagact
      361 cccagggaag aaaggagcgc caccagggag atcgccagca ccatggcaat tcccctttgg
      421 tttcccacca ccttcatcat cacagcaggc ctcctttggc tcgcactatt gatacagcgg
      481 cccggaattt ctcggctgaa tcatggccga ataggtccga tacacgtagc cgcattccgc
      541 cgacccggaa gctgccgccg tccggttttc cacggcgggc ctgcagtacg cggggtcata
      601 gattcgtcct tccacggaat cggctccccg ggtgacgaga gtcaaccgga tgatggccac
      661 ctgccggggg tcaaaggggt ccatggcgca taagcccgcc cccactccgg tccccgcaca
      721 tccggcagcg ccgtaccagt tcccgttccg gtcccgatag gccacctgca tgtccaccac
      781 gttttccatc atgggttgcc gtcccccggt ggttcggtca ctcctccgca ggaccctctg
      841 ttcggtgctt ccggtcggcc ggcattgagc gtccccgttg tccacacagt aatacacggc
      901 tggagccaca tgggtcccgg agaggtttct gtccagtccc aactggggtc cggaggtgat
      961 ggtattgatt ttggcgatgc tgttggtggt ggcatccgag atcacgaact gaaacacccc
     1021 ggccagaaaa tcaggatccc cgctggaatc cccgtcacct tcgtcatcat tgacgtccaa
     1081 cctgccgatg gtgacattgg aggtgccggc gccggagaca atcctttgtc ctcccgcgca
     1141 atcccctcca atcatgttgg cgcgattggt ggcgacgctc aggtcgatca ggctcgagtc
     1201 gttcacatag atccggtcgc tgccggtggc cccgccatcc tcaaaatgga acgccatttg
     1261 gggaagaacg gaccagccgg cctccatcag atcgcgcttg aggatctcca ccatcatctg
     1321 ccccgactcc tggacctcct tgacgttctt gctccggaca tagctcttgt tctggctcat
     1381 gaagatggag atgctcaaga tcatgatcac ggaggtgacc ccaatggcca ccatcatctc
     1441 caccagagtc attcccctca cctgcagcag ggcctttaag gtcgatctca tgggatgtcc
     1501 gccttccatt gcctaaaata gctccaggtc cacgagggtc gaagtcaata ccaaggagcg
     1561 ggtcagtttg gtcttccctc cgccgcgggt ccggaccgat tcgggccagg ttaccgtaac
     1621 cgtgacgttt ttccgccagg tttcgacggt tgtcacctgc caggttcggg taaaggcccc
     1681 ctcggggttg tccgtggagg tcccgtcggc aagtccggca aaattgagga ctgccgccga
     1741 tcgcaggtcc tccagttgcc gattggccag gaagacggct cgaatttcac tatggttctt
     1801 catgtcgatg gacagggagg aaagatggag cacccacagg gccgtgaatg caatggccat
     1861 ggcccccaga gccaccatca cctccaccag ggtaaaccct ctggaatccg gcgcatcaag
     1921 gctcaatttt gatcctcccc aaagaagaaa gggtgacggt gtaggtattc gcggcattac
     1981 taaaggctat ggtcgcaccc aacaggttgg ggttgacgga atcacgaagg gaaatcgttc
     2041 cgcgggcgtt gtagtgaacg ctcagacctg cagaagtgga aaccgtcact ccggggtttt
     2101 tctgggcaac gttcacctga tggaggaccc tctcccccgc atccaaggtc atgctgttat
     2161 tgggatcgga gaatacggta taggtattcc caacgaagac ggccgtatgc ccggtgttct
     2221 tctccatggc aaggaagcgc gcttccttga ggtgaccggc cagttcatag gcggccgccc
     2281 gcgcctgctc ccgtctctgc cagctggaat agccggagta ggcggccccc gtcagaataa
     2341 tcaccacggc aatgacgacc aggatctctg tgagggtgaa ccccctgcaa tttcgtcgag
     2401 gaaaaggaac aggcctcatt tcgtctaggt acctcccatt gtttcccgac gcacctggat
     2461 ctgataggag aggatggctt cggcaccctg ggagtctacg gccgagacct ctacggggaa
     2521 gctccccttc acgtccgcgc ccggcgacca gtgaatggtt cccgtggttg cctctatagt
     2581 cattcccggc ggccctttct tcaaagagaa tgtgacggga tctttctccg ggtccagcgc
     2641 cgagagttgg gcccggtagt tcccgtcact ccccatggtc tggctctgca cctgaacgtt
     2701 tggcgccgaa ttggcgatga ctacgcagcc ggtgacgggt ttcacggaac cggcgctcac
     2761 tcggggactc accaagacct ccacgtaatc gccgcgaaat acgggttttt ccagtttgtc
     2821 ggatgtggat tcctggacca cttgtccatt gatcctccag gagtaggtga ggtcggtgaa
     2881 gcttccctcc agcccctttg ctttgaccac cacgctcaac gactcgccgg tcaccgggaa
     2941 ggcgggctcg atggagacct cttctatggc cagggagtcc gtcggagccg cccctcgagg
     3001 cccttccgtg ctcggagcag tccccggtgc cggagccggt ccggcttgcc gtgcactctc
     3061 cactccctcc acgggccggg ccggaagccg ttgactctgg gattccttct tgccgctcac
     3121 cgggggctcc tgttccgcac aagaaaccag cccccaggga agtacaagaa gaaccatgca
     3181 caacaagaat cttcgagccg gactgtcagg catcccgggt cctccgtttc atgttgtttt
     3241 ctttgacggc tcagacgggc gcacgggcgg ctcccctccg gctgggatac cctcttactg
     3301 gaacaagagg cggaagatgg agaatttcaa ggcattttcg ttgacgacca cgggaacgtc
     3361 caaggtggaa gaaatccgaa cgacatccgt tggggcggcg gcgtagatct ttctgaaggc
     3421 tatgatttga taggagtttg tgctggcgga ttgcacctga acggtgtagg aaccgaccac
     3481 gccgctggtc tggggacacg aggaacagtc tcgaaggcaa ctggtgttgg cgctgtttac
     3541 aaaactggga aggcaaccga tggtcccggc atatcggtga agtgttccca cagcccgttc
     3601 ctcccagaaa acctcctgat ccatcttggc gttcatgagg gccgttatag cctcgctttg
     3661 tcttgctcga ttgatgtaat tgacatacgc tggcattgcc acggaagcca agatcgcggc
     3721 gatggccacg accaccatca attccaccag ggaaaacccc ctctgcaaag agatggagcc
     3781 ttctccgggt ctttgggcat gctgggatcg gtctctcatc ctcgtcatgg gatcccctct
     3841 tttctttgtc tgctccaacc tccttcgccg ggaaagcaga agggctcaga acccctgcgt
     3901 gcctgaatcg tggtgaagag tcggggcggg tctccccgaa gcccccgatg ctctaaaccc
     3961 gcgatcctcc cgggtcccgt ccaaccgtcc gtccaaagga tgatcacctt tcggcaacgg
     4021 gacatcccaa gccctcttta tcttatcggc aatccatcga caagacttca tgaaaatccg
     4081 acatggccgc atgcaggaat tttccggcat tcggttcgag cggccggctc atccgtcacc
     4141 cgcgaacaac cgtacgattc atatcaatcc ttttccctcc cgagagcaag agctttttcg
     4201 ccccctccga gggggcgcgg aaggacgcaa ggtgttcctg ggggaaggcg gtccgcatgg
     4261 acgccggcga tgaaatcagg gaaccggcgc gaggggggga gttttcgaaa aacctttgac
     4321 acccgagccg tccaccaggg accacgggaa gatgatccga aaaacggttc cttcccccgg
     4381 aacgctctcc acctcgattt ctgcaccgtg tttctccagg atctggcggg aaatactcag
     4441 tcccagtccc gtgccctcgg gtttcctggt aaagaagggg tcaaagatcc ggctcctgtc
     4501 ttcctccagg attccgtgtc ccgtatccct gatttccagg gtcagggccg gggggtcgtc
     4561 ccgacggccg gtgttcagga agagctttcc cccttgaggc atggcctcaa tggcgttgat
     4621 caccagattc atgatcacct ggctgatctg gttgtggtcc atcactaagg agggaaggac
     4681 ctcagccaac cggagagaaa tctccacatg ggccttgcgc aggtagacct tggacaggtc
     4741 gtagcagtaa cgcacgatgt cgttgagctg attgggcagg aactggggtt ccttgggccg
     4801 ggcaaaatgg agcacctgtt ggacaatgcg gtcaatcttc cggatctcgt ggaccatgtc
     4861 tccaaacatt tgctcctggg ccgaatccag acggatctca cgccgcagca tctgggcgtt
     4921 gaaattgatg gtagccaggg gatttcttat ttcgtgagcc accccggcgg caagagtgcc
     4981 caaggccttg agcttttcgc tctgttgaag ccgttgttcc agctttcgag cctccgtcgc
     5041 gtccttctca tagtacacga ctcgctccaa taccccctcg aggttgatca ccggagaagc
     5101 aaagtaatga taatacccac cccaccgaaa atgggcgcct tcccgttgga tgcgctcatg
     5161 ggttctcaga cacatcagga tggggcagtc tctggggggc tcttccgtcc cgtagaggac
     5221 ggaagagtat ttctttccga tcaggttccg ggacccgccg tagacttcct cgcaggtgcg
     5281 gttcacccga acaatgcgcc atcgctcgtc aatgacgaaa atgctgtgct gaatggcatc
     5341 aaagctgatc tcgagctcgc tcttggccct ctgcaccgaa tcgaacaact gggcattgtc
     5401 gatggccacc cccaactggt ggccaatggt tgtcaggaac tcgtttcgat cgggtttgag
     5461 cggcaagacg ccgtcgttgg tcacgtacaa caagcccaag acccgcttct gggtcatgag
     5521 cgggatgcag atgatttccg tcacccgctc ttcaatcatt ctttccctca atcggggaaa
     5581 gatgagtttg cttacaggaa accaccgaac gggctggggc cgtttccccg ggtccagaac
     5641 ttcatgggtg agggagaagg agcgaatgca ctccaccagg gaggaggaga atccgtggtg
     5701 agcggccaat tccagaagac catgttcgtc caggccttct ttgaacaaat aaatcgcacc
     5761 cattttgaat cgcagggtac gcaggatcgc catcagtgcc cgctgcaaga cctctttcag
     5821 tacgggagat gcatgcaggg cagaggtaat gtcgaacagg atctggagtt cccgctgttc
     5881 cctcaggggg tcgcccaggg ggataccccc gttgtcgaaa gaatcggatt cagccggtct
     5941 cattggtctc agttgtctgg gaggtccgcc cgaaggcggt aggcgatccg gaagccgacg
     6001 ccggttcccc gcatggttcc aatccctggc ccccgggtcc gcctcccccg gagggggatc
     6061 agctcctgcg tgcgatggca tccgcctggg cgcagaccat ttttgccact cgaatggacg
     6121 ctgcatccac catcttgccc tcgatggcga gagagccgca tccttcctgc tgggccttgc
     6181 cgtaggcttc cacaatcttg cgtgaccgtt ccacatcctc ggcggaaggc gtgtaaacat
     6241 cgttaatgat cccgacctga tcggggtgaa tggcccattt cccgtcatat cccaaggccg
     6301 cactgagcac acacgagcgc ctcaatcctt ccgggtcctt gtaatccccg tagggagcat
     6361 cgatggcctc caggcccgcg gccttggccg ccatgaccat gcgactcaac ggaaagtgcc
     6421 agcgatggcc cggatagaac tcttcccggt ccccatgtcc gctgatcccc ttgctcatca
     6481 ttgtaacgga cgccccgtag tcggcaactc cgaaaacgag ggactccagg cgaggggaac
     6541 tgaacgcaat ttcctcgact ttcagcatgc ccagggccgt ttcgatgctg gcctccaggc
     6601 cgatggggac cttgaagccc atcctcagtt cgatttgggt caacaagtaa tcgatggctt
     6661 ttacttccgc cgcatcgttg accttgggaa tcacaacgac atcaatgcga tcccccgcag
     6721 cctccaccac atccacgatg tcccggtagg caaacggggt gtccatcccg ttgattcggt
     6781 agcaccgcac cttattcccc caatccttct ccttgaggat cgagatcacg cggcttctgg
     6841 cttcgtcttt ctcttggacc gggacgctgt cctccaggtc cagcatgatc acatccacag
     6901 ggagcgcaaa ggccttgagc accatttttt cccgattgga gggtaccgaa agaatcgatc
     6961 gtcgcagtcg tgtaggtcgt tccacctttt ctccttcaac cagagggatc cgttggtcac
     7021 gccaagcaag catttccggg gacggcgagg gggagtgccc cttcgccaac cgccccatgg
     7081 aaacctactt tttctgttgt gcccgcctgt cacgaatggc ggattgggct gccgccaact
     7141 gggccgccgg gactcggtag ggagagcagc tcacatagtc gagccctatg cgatggcaaa
     7201 aatcgatgga ggacgcttcc cccccgtgct ctccgcagat cccgatcttc aggtttggtc
     7261 gggtttttcg ccccttggcg acggcgattt ccatcaggcc tcccaccccc acctggtcca
     7321 gtcgatggaa aggatcatgc tcccagatcc gggccgtgag ataggggggc aggaatttcc
     7381 cggcatcgtc ccgactgatt ccgaataccg tttgggtcag atcgttggtg ccaaaggaaa
     7441 agaactcggc tacatgggcg atttcatccg ccgtcatggc tccccgcggc acctcgatca
     7501 tggttcccac acggtactcg accttgactt catactgctt cataacctcc ttggccactc
     7561 ggtccgccac ctctctctgg tgcttgagct cgtttacgtg gcccacaaga ggaatcatga
     7621 tctccggatg cacgtcgagg ccctctttct ttacctcgca cgcggcctcc atgatggccc
     7681 gtacctgcat ggccgtgatt tccgggtaga caatgcccag gcggcacccc ctgtgtccca
     7741 gcatcgggtt ggcttcatgg aggctctgca ccttggaatg aatctgttcg aaactgaccc
     7801 ccatcttggt ggccagtttc tggatgtcct tttcctcgtg gggcaaaaac tcgtgtaagg
     7861 gaggatccag cgtccgaatg gtgaccgggg catctttcat gactcgaaag aggcctttga
     7921 aatcctcttt ctgcatgggc agcagtttgg ccagcgcctg ttctcttccg gcggcgtccg
     7981 cggccaggat catctcacga acggcgtcga tccggtttcc ctggaagaac atgtgttccg
     8041 tcctggccaa cccgatccct tcggctccaa aggcaatggc ttcctccgcc tggttgggtt
     8101 gatccgcgtt ggcccagact tccagcttgc ggaattcatc cgcccacccc aaaagggcga
     8161 tgaaatcctg atagacttgc gaccttctcg gagacaggct tccatcgatg atgaccctca
     8221 agatctccga aggccgggta gggatctgcc ctgcaaacac ttctcccgtg gtgccgtcca
     8281 gggagagcca ttggccttcc cgaatgaaac ggtctcccgc tttcatgcgg cggccttggt
     8341 aatcgatctc gatggcattg catccaacta cgcacacctt gcccatctgg cgggccacca
     8401 gggcggcatg ggacgtcatg cccccccggg ccgtcaagat tccccgagag gcatgcatgc
     8461 cccgaatgtc ctcgggggag gtctcccacc gaaccaggat gaccacttcc ccgtttcggg
     8521 cccactcctc cgcatccgcc gcattgaaca ccacccggcc cgtagcggcc cccggccccg
     8581 cattcaaccc tcgggccaga aaggccccat cccgcacggc ttcctccttg gccttcgggt
     8641 caaaggtcgg ccgaagcaac tggttgatct ggtccggcgt cacccgcatg agagcttcct
     8701 ccttgtcgag gattccctgg cgaaccatat ccacggcgat tttgaaggaa gcaaacccgg
     8761 tcctcttgcc cgaccgggtc tgcagcatcc agagcttgcc tttctctatg gtgaactcga
     8821 tgtcctgcat gtcccggtaa tgtcgatcca gcttgacccg aatctcatcg agctccttgt
     8881 agatctcggg catgtattcc tcgagggacg tctgatggtt ctcggctttc tgcacattgt
     8941 tgatgggttg cggcgtccga atcccggcca ccacatcttc tccttgggcg ttcaccaggt
     9001 attcgccgta gaaaacattc tcccccgtag cggggtttcg agtaaaagcg accccggtgg
     9061 cgcaatcatc ccccatattc ccgaaaacca tcgcctgcac attgaccgcc gttcccatgt
     9121 cctccgggat ttcgttcaac tcccggtaag caatggcccg gggattgttc caggacccga
     9181 acaccgccgt gatggctccc cacagctgat ccatggggtc ttggcgaaac tcgagctttt
     9241 tccgttcgcg gatcagggcc ttgtaacgtt ccaccagttc cttcaagtcg tgggggggaa
     9301 gctggttgtc gaactccacg ttccgtctct tcttcatttc ctccaggatt tcgtcgaagg
     9361 ggtccttctc gtccttggag tcgggcttca gtcccaaaac cacatcgccg tacatggcaa
     9421 tgaagcggcg gtagcagtca taggcaaagc gttcgtcccc cgtctgctgg atcaagccca
     9481 caacggtgtg atcattcaga cccagattca ataccgtgtc catcatgccc ggcatgctgc
     9541 ttgccgcccc ggatcggacc gacaccagga gaggattctc ggggtttccg aactcccgtc
     9601 ccatgatctg ttcgagacga tgaagggcct cctccacctc cgctttcagc tccggcggat
     9661 aagtaccgtt gtgcttataa tagtacgagc aaacctcggt ggtgatggtg aaccccggag
     9721 gaacgggtat cccgaggttg gccatttccg caaggttggc tccttttcct cccaacaaga
     9781 gcttcatctg ggcatttccg tccgccttac ccgcttcgaa gaagtaaacg tacttaggcg
     9841 ccatcattga attcccccta ttgttcatgc ctttctgaaa tactaatgga ctcggccaac
     9901 cggttggatt cctcattccg ctgatattct tcgtgcgacc gaagagacca cagggcttcc
     9961 gcgtgagaca tcatcagaac aggatggatc gatgtgagca ggtttcttgt ctgcggtccg
    10021 tcccctcagg tgagtgggcc gtattttacc catactgtcc cctgtgcaca atccgtaaat
    10081 gcactatttg cggagggaga ttaaaagtgc gggggttttt cggggaaatt ccggcagacg
    10141 tcatgaatgg attcgtcccc ctcgaacctc cccccggaca tcaaacgttc gaggggggct
    10201 cgacgaaggc ctcgcgtacg acgaggcggg gcgggtcgtg aaacccgccc cgcgggaaag
    10261 gaacgtcgcc gttatatctc cagccatgag tcggaataca gggagtgacc caggatcacc
    10321 ctggcggtct tgacgtactc ggtttcttcc cgggaaaggg agctcaggtc gacgggcacc
    10381 ccgtcttcca ccttgtgaac caacgcctcc aattcgggac gcttcccccg agcgatctca
    10441 tggagctctt tgtcaaaggt gtccacgatc gccgaggcca tgccgtgccg tttccatatg
    10501 atcaacatga cgcggttcag aatgggtctc agatggtcgg gggggccgtt tgagacattg
    10561 gacaagccga tggtgctttg acaccccggt gcaatgtccg gaaacatctt catgaactcg
    10621 agcacactca tgatctggtt ttgctgcaca ttgaccggag tttggatcgg gtccacgtag
    10681 atgcgctccg gtgcaatgcc gtggtcggtt gtggccgaaa aaaccagctc cgaacaaagg
    10741 gcccctcttt cgttctcgtc ccgcggcatc ccctcggggc cccagagcaa acccacgaaa
    10801 tccgcatcgt attgggccgc caagggaagc agggcactca tccgttccgg gcgaaccatg
    10861 atggaattga tcagggctct ccctttcttg tggactttga gtcccgcctc gatggccttg
    10921 atattcgaag tgtccagtgc caggggaagg tccaccactt catgaatcac cggaaccagc
    10981 caggacatca attcctcgcc gatctttccc gccgggccga tgttgagatc gatccaatcc
    11041 attccggctt cggcttcctg gacggcaagc tcctgcaagg gttttgcatc cttttccttc
    11101 agggccttcc cgtatttcct gttcatgata ttgaggtttt caccgatcag tttcattcac
    11161 gactcctcct tcttccggag aacccgacgc ccgctcttcc gggagcgttt ctccgtctgc
    11221 cgccccttca ccaggtcttt ccggtttcaa gccctgcaag gcccggggga ttttcgtggt
    11281 ccggggcttt tgcaggcccc gacagcggac ccttgaagac ccccacgggg tcaaagactc
    11341 ggttcgcgcc gaaccgaacc ggtttttcca ccattaagcg gctttccata ccttcaggaa
    11401 cttagggatc tggctcgctt cccggggacc caccaccacg ttccaccccc ccagttcctc
    11461 ctcgagctcc ccgcttatgc ccgcggcata tccgggaatc acgatggtcg tgtgtttcac
    11521 cttttccttg attccgcact tgttgataaa ggagcccacc gtctcccccg aaaacttccc
    11581 ggcagcccag gccgtcatga cgctgagtcc ttccgtatcc tgcacgcaga gccacgaggg
    11641 gacccgactg gcttcgatct cccccgacac gatgaagtag gtgagggaga aattgcaggt
    11701 gaccaggacg ggagagtctt cacccggatt gttgatggga taaatccctt gttcggccgt
    11761 catggggcgt tgggggtcgg tgtaaatgtt caggcgactg agaaacagag ggaacaggct
    11821 ccaaggctga aagtcgctca aaaccaagat cgccccgtac ttggcgatga aggtggccgc
    11881 aaccagagat tcttccgtca ggttgtccgc catctcattg gcaaggatca tcgtgggaaa
    11941 accgagggga cgaaagcggt ccttgagggc cgcgcgccgg atcagtattt gctcctcgaa
    12001 cgccttcttg agatcacggg tccccgtgtc gatgacaagg tccttcaggc ccatctggga
    12061 gagtttttcc gtgagcccga tcacactgtc cagggtcccg tcgcctttta ccgccaacgg
    12121 acaggcagct tccttggcca gcgatcccaa ggcctccgca tttccgggcg ttgcggcata
    12181 gagcagcggt ttcttgtcct tcagctccga aacggctgcc ttcatcacct ccacatcctc
    12241 ggacatgagg atcaagctcg ctccggtgcc gtccgagacc ttcttggcaa gggccgtaaa
    12301 ccgggaggca tccttggatg tatccttcac ggcgaacagc tccgctcgaa gcgtcaaacc
    12361 cactcgttcg taccggcatt tcttgaagcg ctccatcttt ccgtccacgg acccggcgtc
    12421 ctcgtcggtg gtgacgagga cggcaaagcc cggcggattc acaaaggtct tctcgtggcg
    12481 gaacaagacc gtttcaccgc caatcttcag ggcgcattct cctgttccca cggtaagggg
    12541 gcggatggga ggcgccgatt ccgcagccag tttttccttg gcctcttccg aaacatacgg
    12601 acacttgtcc aattccgcct ttcctgcggc gagattcatg gcaaaggcca ggcacgtggg
    12661 aaccccgcac tctccgcagt tcgtcttggg tagcaattta aagatttgaa tgcccgtcaa
    12721 gcccatctgt tctcctctcc tcatctctca acggggttga gtccaccttc gggactcacc
    12781 ccaagattga ttccatgctc aaagccggat gcccgacgga atcgagaaag gccatgattt
    12841 cctcctccgt cactccctgc tcttcggtgg caatcatgtc aatgaaaccg gggaccccga
    12901 tttcttctcc cctcttgatg agcctgtccc gaagctcttc tttcagcatc ttgggcatcc
    12961 acacgagcct ctggaagcct ccgtctcccg cgatgtactt gcgctgggtg atattgtact
    13021 tgctgtgccc cacaaaaccc ggcgtcacgt ttcctccgcc gatggtcccg gccagggtgg
    13081 tgaacttcat gccgcagggg gtcattccgt tatagtcccg gttgaccgtc atcaccccgt
    13141 tgcacagggg caataccgcg gcaatacatt cacaacaacc gcaggtggtc atggggtcgt
    13201 aaacgaggct gtagaaattg tagctggaaa tggactggcg agaggccttg tagacaaaat
    13261 cgttcacacc cttccagttg ccgtagcgtg aatccagagt ttctcccttt tcaatgggct
    13321 gattgggacc cgtcgggttg atctcgtagg atgccttgca gtccaaccag ttgtaggcac
    13381 cgcatagccc cgtcctttcg ggactgatca cgcagacatg ggtgggggca aaggattggc
    13441 agagggtaca agaatagtag atgtccgtgg tctcgtcggt catcccctcc acacgatcat
    13501 cccgtatctt gtaggcatgc tgggccaggg gaagggtttc cagcaccttg gcctcctcag
    13561 tgtagatttt gacctgacat ttgtcgaaaa tggcaccgaa gtcctgatga tacttggcat
    13621 gcaggatctt ccccaggtgc tccagggtga accccttttc cacggcctgt ttgctgatgc
    13681 gataccaggc gatgtccctc tgcccgatgt gcatgattcc ctgggcataa ttgatgaggt
    13741 ggtgaatctg cctctcgagg atcggctcgt agtccgcctg cattttacgc cccgccactt
    13801 ccaccacgac cgccaggggc agccggcttc ccggcttcac atccttgatg tccgggccga
    13861 caacctccac ccgtccgtct tccaccgcat ccatttcggc aatttggacg agctcgcaac
    13921 agacggtctt cccgccgccg cattccaggt aaacatcatc cttgcgaacc ctctccccct
    13981 cgaaggccgg tccgtaggat acgggaacag gcacctccgt caccgttacc ttgagaccac
    14041 gcacttcgat ggacttggcc gtcatgtgat cgtggggaac attggccacc acgtgttcgt
    14101 aggtacagat tcccgtgggc agaatctcgg gaatgtccgt atcggcgatg gtggggaacc
    14161 cccagttgat ggctcccgcc gcgttggccg cccattcggc gtttacttcc cccaatgcgt
    14221 tcacaaaggc gaagatccga tctttgttgt atttgaggac cgtcctgtaa tctccgggct
    14281 gaaccccgcc aaaggccatg gccgctcggt tggcaaagcc cagcgcaaaa acggcggcgg
    14341 agatgtcggg accaaagggg acaattcgag tgccccaacc gatctgaacc cccgcctgga
    14401 ccagctgctg ggcacaggtg gtcccgtttt gattggcaca aaggaagata tagagattct
    14461 ttctttggta atcctcgacg atgtccttgg cgatttccgg ggtaggggcc gcacccacga
    14521 tggccgcaaa accgggggcg ctgccatcca cgaattccac ccctcgtttg cgcatgatga
    14581 cgtcatccgc cgcgcccagc cacaattttc cgttctcgat gtcgcagtct tccccttgga
    14641 ggtagaaccc ggggtcctcg acataccgaa tggcttcccc gatttcttcc gccaatatgg
    14701 ccgccatccc ggcatccaag gtatgcccca aataggggac gtggatcttt cccttgatgt
    14761 gggggggcag caactccttg catcgcttga gaacaatctc ggcatcggcc agcttctcca
    14821 ccttgattcc caggatggaa tagatgaccg gcaggtaata agccgtgttg ggaaaggcca
    14881 gaggctgatt cggaccatac ttgtccatcg ccctccgaaa actcccttcc gtcttcgcca
    14941 caatgttgta cgcgccctga atcgcggcaa atgcgactaa cctcgacatt gctctactcc
    15001 tcttctcaag ggtacttccc ttgatcctgc agcggtttca caccggcatt ggcacccaag
    15061 gggaccgcac cgaagaacac ggacccccgg cattttcccc ctacgccctt cgatcttcca
    15121 tactgaacag cttgcgctcc cgctctccca tgattcccag ggcctctcgt ttcttgtcga
    15181 tatggcggat catcatgtgg gctatctcaa tgggatcctt ggcaaagccc cacttcccga
    15241 atcccagctc ttccagccct tcgaagagca actgatggaa ctttgttccc tcgacggagg
    15301 ggaaggtaac gccgaagacg gtgtacacgc cggacgccac gaagtactgg ccgatgcaga
    15361 tggccttttc gctgtagtat tcgggggccg cgcccgccac gggaagatcc gcaatggaat
    15421 tgcccaggcc gcccacccgc accatttccg cacaggcgat gagaatgcgg ctgttgtcca
    15481 cacaggaccc caggctcagg accggcggca tacccaccgt ctcacagacc tcctgcaatc
    15541 cttctcccgc caaaaccgcc gcatcgggtt tcatgaggcc cgcttttgcc agggcgatct
    15601 gggaacaccc ggtctggacc accaggacat tgtttttgat cagttccttg acaagctcga
    15661 cgtgggcgta atcctgtttc acccgcggat tgttgcaccc cacgacgcct gccacacccc
    15721 ggatacggcc gttgatgatg ttctcgttga gaggcgtgta ggatccgcga aaagtacctc
    15781 ccagcatgta attgacgtat tcgtgagaaa agccgtggac tcccaggtcc cggcgttggg
    15841 gtatgacgat cttggcggga cgattcttga aacgatccag cgccatggcg atgatctttt
    15901 cggtggactc ccggggggtg tgatgattga attcgatgtg ggcggcccct tcgatcttgg
    15961 ctcggggatt cgtggtgaag aacttggtgc cgtagcactc ggacatgctg gaaagtgcct
    16021 gcatgatgca ctggacatcc actgccatgg cgtccaccgc gccggtggcg atgacggcct
    16081 cggtgctcag aaagtttccg gcatggggaa ctccgtggcg ccccaaaacc tcggctcccg
    16141 aacaacacat gcccaggagc tgaatccctt tggccccggc ggctttggcc ttctcaaggt
    16201 tggcaggatc attggccgag gcgatcatgg cttcaaaaag gagcggctcg tgaccatgaa
    16261 tgatgatgtt cacgtggtct tctttgagat agcccatgtt gacgcccgca accagcggtt
    16321 ttggagtgcc gaacaggatg tccgagagtt ccgtggccac catggacccc ccccagccgt
    16381 ccgccagggc ggtcctggaa cactgtttca caatgttttc atagtcctga tccactccca
    16441 tgtgggtgcg atgcatgatt tccatgatct cccgcatggc tccgcgaggc accacgccgt
    16501 gctttctcca caactccagg gtcttgggcg gaacgcgctt gacaaacggg atttcgccct
    16561 ccacttgggt gtaggtcttc tccagctcgt tgtagagatc gacggcgatc tctttggtcg
    16621 aacgccccgc cacggcaatt ccgatactcg atgccacatc ccgaagtttc tgttcatcct
    16681 tgatctgaaa ctcctggatg ttgcctttta ccacctcccg gaatacgtcc aaaatcccca
    16741 tcccgtggtc cgtatgagct gacccgccgc tggcgaccac cctggcaaag ttgcgggcct
    16801 ggatggtatc gatggtggct ccgcacacac ccaccttgga gtaagggtct ttggacacca
    16861 gccggcacgg tcccataaag caatgcttgc agcaggcgga atcggccccg atggggcagg
    16921 gcttcatgtc ggccgccctg ctaaaggcag tctcgacccc gtcggtcgct gctttctgaa
    16981 gcatctgtat ggtcgcttca catgtgctca catcccggaa attcagttgc ttggtcttct
    17041 tttcttcact cgacatgatg cacctctttt tccccttcct ggggcactca aaagacttcc
    17101 agcagtccgg atttcaaaaa ttccacccgt ctccttctag accattgttt ggatatattt
    17161 cttcacgagt tgcaccgagt ccggatggcg caggacgacg atatcggccc cggccagcag
    17221 gagggaaacg gcggtgatgg tttccatgag cacaccccgt ttttggggat ctcccaattc
    17281 cggagcttcc tccaggctca aattggcctc cttggctttc caggactcgt tggcaacgtt
    17341 cacaatgacc ggctgctgca acttctcgtc ttcctgggtc aaggccgcct gttggatacg
    17401 ctccaacacg gaataggtat attccaaacc gtagcccaaa ccgcccgtgg tgggatcaat
    17461 gacgatttcc gtgccttgga ccccgaggtt tcccagaaga atattcaatt gcttggccag
    17521 gttcacgtcg atgggcgaag aggcgacgac ggtgtgcccg tagcccaggc aagcggctcc
    17581 gatttgtttg taatccccct cctccaccgg ggccaccgcc aagtgcctgc cccccgactg
    17641 ttccgtcacc aaccgcatca cttcgacgtc cttctcatgg ttggccaccc cccaaaccac
    17701 cacggggacg tccacggcct ccacgatctt cttgaccgtc gccgccacgt ccgcggcagg
    17761 tcggttgtcg ccgttgggat cggcgctttt ggtttgaacg acgatcaaat ccgctccgta
    17821 ctcgttgacg cacttcgtgg cccagagcgc tgggtccccc aggacctcct tgaagggttc
    17881 tatggccacg gccgaccatt ccgaagacgg gtccttgtcc cacacttcca tggccaccct
    17941 cgggcggtgg ggcatttgcc cttcgaaggt gtggaaggag taggcagtcc gccctccaac
    18001 ggtgacggcc ttgttaccca gccccagcgt gacctcgcgg attttccccg aataaacctg
    18061 ttttgaaggt tgaaatgcca aggtagatcc tcctttcatc ttgtgctatt tttcataaat
    18121 ccgagggtga agatggcggc gctccggtcg caggactcag gacactcccg tccatcggca
    18181 aacgtttcaa aaaacgacgg cggcccccac catgattcca gcggctccgc tgcaaatccc
    18241 ctattcccga ggaccttgtg ccgttgcaca aaacttttca caatccgagt gtgttggatc
    18301 taatgagacg cagcttggga aagggtaacc atccgcaggc agaggaagtg gatccgaagg
    18361 ttggggcctt gacggcaggt tcagcgggag gagtgcggtt cgttcatcta gaagtaacta
    18421 agggtatttg ctgatcagct tgtgacgata cctgaatatc gaaacggcac tgaaatgtca
    18481 actcgtttta cgggtcttgt ccatttttgc acaaaataga gggctcgatg ggggaagaat
    18541 caggcccttt ctggaaattg cccaaccccg gcgatcccat ttcccaccgg gtgagccaat
    18601 cccctttccc acaaggggga cgacccggtc ggctccggat acgcggccgg ctctctgccc
    18661 cacgcagccc ggaacacccc gaggctcaga agcagctggg cacgtggagc gctcgccttg
    18721 catcgccctg ggttcggacc accgagtcca ccctgagttc cctctgcaca cgctcaacac
    18781 gaatgttgga ccccgcccag gacaatcgaa ccgtctggta cgggttacac aaagcacctt
    18841 gatgaagagg gagccgcata aacgtaaacc gaatcatctg atcggccgac cagtgcaaag
    18901 gataatattt ccggggatcg aactcgaaaa caccgtgaaa caccataaaa cgcacaatga
    18961 cgtgcctggg aggcgacacg acggccttcc tcagtcttct gagaatggac cgaatgcgaa
    19021 ggcgatcatg gaccgccagc accacctggg tcggaaactc gtcgccgcag gtgaggcccc
    19081 cgaagtttcg tttcacccac gccacatcct taagaattct cctggaatcc caaaagtcca
    19141 gatccgtgaa cacgtggaaa tcatatttgg tgaaagcggg atggcagtaa atgattctgg
    19201 ccatcatacc cccctgcttt cccggattcg tcctgccgcc gcgctacccg cagccgcagc
    19261 cgcaagggca gccgggcggt tccgcaccga agctcaccca tgcaattttt tccaaaccat
    19321 accgtgacaa gagttcccga atttcgccca cccccagata ctggatgaaa ctctcccgaa
    19381 cctgtggaat ccggggaaca aggctctgca ccaactcatc catcagggca tgggaagggg
    19441 ccacgcatag ggtgtccaac cttccctttt gcccgggatc gctgaccacg cacagaggga
    19501 accacgcttc ccgggaatgg aggaacccca cataaaacat ttctctctgc acggtggcaa
    19561 aaaacggctc ggactccagg acctcaatca tcgccaactc cttcgggcag gggggggtgt
    19621 gttgggaact tgggttttag cataccccca agcggggctg gtttc
//
LOCUS       DAOOVC010000062        19370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1917_length_19370_cov_5.981621, whole genome shotgun sequence.
ACCESSION   DAOOVC010000062 DAOOVC010000000
VERSION     DAOOVC010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19370
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1917_length_19370_cov_5.981621"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1143..1625)
                     /locus_tag="PLM79_09580"
     CDS             complement(1143..1625)
                     /locus_tag="PLM79_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86601.1"
                     /translation="MGKRFTGSAMFVLLIVALTAYGCATPGVNLVENRTASVEIISAG
                     DEKAFLKETDVYQDGTELVVTGYVKRKSRWSLPRDYDAHMDMAVFTSDGKLIDKASAG
                     FLPMPRVKSLQSPFTARFQLVAPKGTTVRFAYHRFQSKPSKTFDCGQNAVILGTDSRD
                     "
     gene            complement(1684..2154)
                     /locus_tag="PLM79_09585"
     CDS             complement(1684..2154)
                     /locus_tag="PLM79_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86602.1"
                     /translation="MSTKTLSKGLTGLFIYLVALSASGCTTGYDLLDTGMGSIELVAS
                     RDRYAAITETELYQDGDCLLVTGSVEEISADFLPAGYDAHVDVAVLAPDRKVARKRSI
                     RISLAEGVQSDFKVRFPYFAERGTVVRFAYHSDRRLADGAPNCGENAAVLHKSG"
     gene            complement(2173..2514)
                     /locus_tag="PLM79_09590"
     CDS             complement(2173..2514)
                     /locus_tag="PLM79_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOV86603.1"
                     /translation="MKEIKAYIKPHKLSQVTLALQKIEGLTGMSISNVKGFGRGRAKT
                     VKERITEDFIDYVQHVKIEIVCRDELVDQIVSTIEKEAHTGLRGDGKIYVSEVTAAVR
                     ISTGERGEKAI"
     gene            complement(2527..5667)
                     /locus_tag="PLM79_09595"
     CDS             complement(2527..5667)
                     /locus_tag="PLM79_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HOV86604.1"
                     /translation="MNKLIQFVLLNRLLMIVLGAMVLVAGYASYRHLPVDAFPDVTPV
                     LVQVFTETEGLAPEEAEKYVTYPVEVAMNGLPNLKEIRSISNFGLSVINIYFEDGTDI
                     YFARQLVSERLQLAKEEIHEGFGEPEMGPISTGLGQILFYFVEDETGKRTPQEMREIQ
                     DWLIKFNLQTVPGITEILSLGGEVKQYQVQIRPSELKRFGLSIGDVVDAVKANNSNVG
                     AQYIVKNAEQYLVRSVGLAKDIADLKGIVLKTVDGTPIHLHDVADVVIGGELRQGLAT
                     RDGKGEAVVGMVLKLIGTNTSDVIQAAKTRMDEINKVLPPGIKVVPYYDQATLVAKCI
                     ETVKDALLEGIVLVTIVILAFLGGIRPSLVVAVSIPFSILFTFILMKAFNISANLMSM
                     GGLAIAIGMMVDGTIVMVENVDRMLNEADRSESRLHIVVRACAEVGRPIFFSILIIII
                     VFLPLFSLQGVEGKTFKPLAYTLSIAMLGSLIFAIFLAPVASYLIMRRPKAGKRGDHH
                     HEPFLIRMLLKLYEPVVRFFVRMRFAAILLAVLILAAGLLIFPRLGSEFVPRLNEGDL
                     LVRATMAPSISLDEARDTMLRFERQLMEKFPEVTRVVSRVGRGEVGAHADPVNSAEAF
                     VGLKPIGEWTTTQKPEDLYALMSEAFENFPGVQFNFTQPIAAAVDELLTGTKAELAIK
                     IFGTDTEVLKEKAGEVEQVVRKVKGAADVQKDQTTGTPQVRISMNYPALARYGINVET
                     VQNMIKTAVGGEKAGQIFEDVRRFDIVVWYEPKSRDSVEAMRRIVVKSPAGVDVPLAE
                     LTTIEEIVGPRQVTRENNQRFIAVQCNVRGRDIGSFVADAQQAISAEVKLPPGYIVTW
                     GGQFELQQEANKRLALVVPVTLLIVFLLLFSSFNSLKNTLLILLNIPLGLVGGVAALW
                     ITGQNLSVPSSVGFIALFGIVLANGMVLVTYINQLVSDGVPIDEASIRGACLRLRPVL
                     MTALCAMLGLIPLLFSSGTGSEVQRPLATVVIGGLVTSTVLTLLVIPAIYKWFAIKVE
                     LETPSAEKLHTLPKDE"
     gene            complement(5763..7163)
                     /locus_tag="PLM79_09600"
     CDS             complement(5763..7163)
                     /locus_tag="PLM79_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV86605.1"
                     /translation="MKIKSALWIALAVAGVMLAVLAFRSYGPSGLLLAAQGEKAGSHA
                     GHDEPGAKARAESAHPEEQADAHAGHGGEKAKGKHKEDTSSSAKAGGHADEEKAAGAQ
                     KDSHGDEVEEKAIRLSEAQIKEFGIEVSAAGSGELQNFISLPGEVVLNEDRKAHIVPR
                     IAGVVREVRKTLGDTVKQGEVMAVIESRELADAKAEYLAAQEKTSLAQASYRREEGLW
                     KKKISSQQEYLDAKQALAEAQIALRSAEQKLFALGLSRDHLKQVPQHADSSLTRFEIM
                     APFPSTVIEKHISLGEMLKEDAECFLVADMTTVWVNINIYQKDIASIRKGQRVLISLG
                     QDGGGQEAEGQIAYVEPIVKEETRTARARVILPNPQGNWRPGMFVTAKVAVDSASAPI
                     VVPSSALQTVEEKQVVFVQRAEGFEARPVVVGRATNGDVEIASGLSAGEKYVSKGTFT
                     LKAQLSKGAFGDGHNH"
     gene            complement(7176..8570)
                     /locus_tag="PLM79_09605"
     CDS             complement(7176..8570)
                     /locus_tag="PLM79_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV86606.1"
                     /translation="MRLMYMRGVSVLGCVLLLIATAGCAQKRVTGLAKPEQDIGESDL
                     KTPAIPQKSAEPGMDTVPAGEPTGVLTLRDALAHALMKNPELAGTSWDIRAAEARKLQ
                     ASLLPNPEAGIEVEEFAGGGDRSGFDVATTTFQLSQLIELGGKRSKRTQSAALEQDLA
                     GWDYQAKRLDVLIGTTKAFIDVVAAQEQVALAEELVRLAEQVVYSVSERVDAGKVSPL
                     EEVKSRVELSNTRIDLEKAKSSLKGSRKRLSSIWGQDEPTFEKADGELDTVKAIPSRE
                     QLTNLISKNPDVARWVKEMEQRTAALEVEKARRIPDLTVSGGVQRFNDTDDHAFVIGV
                     SLPLPLFDRNQGGIKEAQHKLAKAKEESRAAVAKANTGLADAYQELSASYEGAVSLKG
                     EVLPAAELAFQASGEGYREGKFNFLEFLDSQRTLFEARGKYIETLATYHKAAADVERL
                     TGEKLDAAMETPSK"
     gene            complement(8707..9165)
                     /locus_tag="PLM79_09610"
     CDS             complement(8707..9165)
                     /locus_tag="PLM79_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86607.1"
                     /translation="MESVKFFKIGRQRRFTLWFSLALSLWLVVGSAESAFLCFGANDH
                     LRLEFLRDAGCNQVPEKAAFSFLENSSPASPSAGNDCGPCTDIALSITSALYPSAPNP
                     IIEQQPLFADSLPVLIADCAETPGTSLFAGQQPVTSGLVLARLSSVVLLI"
     gene            complement(9947..10069)
                     /locus_tag="PLM79_09615"
     CDS             complement(9947..10069)
                     /locus_tag="PLM79_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86608.1"
                     /translation="MSIPQRRVMAEVVMVEGMVAVDTGMAGKVVWGARNTADNW"
     gene            10598..12481
                     /locus_tag="PLM79_09620"
     CDS             10598..12481
                     /locus_tag="PLM79_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86609.1"
                     /translation="MHQERTVFLIKKRLSHRFFVALMLISLIPIGLMGVQAHALARRA
                     LTASAFLHMETIAKDHARHLDAWLKERLGDIGVLARLPAMREALREYCCTLDSVAPSG
                     ELTALLKDTITATRERSSSYESIYVMLPGGGIIAATHPPSEDFFELQRDGFIQRVMDG
                     SEPAFGSLCRGADSKWHMPVGAKIRDRDGTTLALVVAVLDVSQTIDPVMNDRIGLGET
                     GETYLVGKEGKIISESRFLSQSETTKQTFDTLGIRAVLEGRQGTAIYSNYLGREVLGS
                     YLWLPRYEWGILAEMEADEILWPLKWIKAASILTALLVSSACLLMAYLVSRRIAKPIS
                     DVADAAQRMSEGDLDQRITFSSQDEVGMLAGSFNTMAQQVSRLIHSLHQKEESLQKAY
                     DELVATQNQLIQSERMAAIGELVACIAHEMRNPLSSVKLNLQIMEMDLKEDDVLSEHC
                     SIALDQVGQLEGMFSDLLNYSKPLVLEKRSVVVEELVNRGLEQLSGEIASRDIRVIRT
                     NHMDLLAVTADADKIVQVLVNVLKNAMQASPQGSEIRITIQEDEAPQGRVSEIIITDH
                     GPGISARNLQNIFQPFFTTKKKGTGLGLCIVKKIMDAHRGTVSVSSEEGKGTTVRLVL
                     PVA"
     gene            12491..13858
                     /locus_tag="PLM79_09625"
     CDS             12491..13858
                     /locus_tag="PLM79_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86610.1"
                     /translation="MRKILIIDDDPSLARTLELYFRRKKCEVSTSGKGKEALELWASE
                     EPDLILLDVQLPDMDGPDVLAMAKKEQLKGEVIMITAFHNTEATLKAIRLGAIDYLYK
                     PIDRQALDLLLEKTAVQKKQRERAAKLCHVISESYRSEQIIGRSSGSLEVIKAIAQVS
                     QTPTNVLIEGETGTGKELVARTIHQESAPEEPFVAINCAAIVGSLLESELFGHEKGAF
                     TGAVQRKVGKLECAGEGTVFLDEVGELPLEFQVKFLRVLQEREFQRVGGNKTIPLQAR
                     IIAATNRDLHEMVQEGAFREDLYFRLKVFVIRIPPLRERTEDIVPLTEYFLTRLNQEM
                     HKRVVRIPNAYLEALRTYGWPGNIRELQNVLRRGLILSRGEVLELNETWLTAGETPLE
                     AEGRREVPQSLADMEKAHILKVLEYTDWNYGEACQILGISRPTLRKKIADYGLSSEGD
                     ENGMV"
     gene            13885..14244
                     /locus_tag="PLM79_09630"
     CDS             13885..14244
                     /locus_tag="PLM79_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86611.1"
                     /translation="MRIVLVENDPVLLKTLEMLLRRQGCQVVAFHEPAMACAFLERGE
                     HFDLLIIDYMMPELTGREVLERVRGRFPKGCRVVLISGHTDVVESLDLKAMGVDTFLP
                     KPLDLKRLRQTISISSG"
     gene            complement(14351..15247)
                     /locus_tag="PLM79_09635"
     CDS             complement(14351..15247)
                     /locus_tag="PLM79_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86612.1"
                     /translation="MKSKAPHFTFGLLVFATFLCFSPVFAYESSHDGGGHEGEHGGTE
                     TPCSTCGQTTHHTFDHHSQASHPEDIVKHLLHPAIPGDCLSSHFNRETEHAGTTKEIH
                     YDAQGNPDYYYTMAYDAAGRISRIEHHSSFEGLQGYTICRYNSSGLLVRTLNYDMADA
                     QTGYTQYVYNNAGKLVTARTYEHGSLSYTTKCSYNSLGRLVKVSQYDAGGALQWYRTC
                     QYNANGHLTKTMHRDPAGRVIESKSYEHNGLGQLNKVLEHNADGVQIGSCSYTYDANG
                     QTIKAACYDPNNNPLGRAVCSH"
     gene            complement(15485..15658)
                     /locus_tag="PLM79_09640"
     CDS             complement(15485..15658)
                     /locus_tag="PLM79_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86613.1"
                     /translation="MFKRILAAAVLAITLTVSSAPLWAAMGSMDQYTTVEHRGHRGGH
                     GGGHGGGHGGGHR"
     gene            complement(16304..17527)
                     /locus_tag="PLM79_09645"
     CDS             complement(16304..17527)
                     /locus_tag="PLM79_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86614.1"
                     /translation="MFRIKGLQQRVVFFFLVPPAVIMIGIGTTVLVYASENMFKECRK
                     ATFLKLTPTAHSRDMSQRPKNPSVEMHHEAAEEMQGSGWRKTYEFYIVDSGGHIRDRT
                     TSGHEKVELDNGPMKLTPSVVIKGTWLGLPAKISPVANVTAEKTMKLSEPPSTNTQSV
                     PRVSEAVPMQSPEPSKPVTKLTWDPPNKLDMDWYERKAREFGFNGLERSRALSKERFK
                     DIMDAIGRDWTRVRNDFPGVEIIQANRDKEPNPHFPRKLFIYAGENLPGDVYDGAARC
                     CYDCGSIHIAAGLLDFFRIEQYRLGNEFAYTSCGLGTTRHEFGHMVHDFLPKELSEEW
                     DSVYESVGGELTESKGRMSSESPVSVSISKYATVNQKEGFAEAFSVFTDPNYGRGEDK
                     RLPNAVESFFEKILK"
     gene            complement(17619..18662)
                     /locus_tag="PLM79_09650"
     CDS             complement(17619..18662)
                     /locus_tag="PLM79_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86615.1"
                     /translation="MIEIRQKEKVILVSALLAAVTVLHYATGIQSHHLHIIYQGLYYI
                     PLILAAFWFGLRGALAGSVGITLLYTPFILIYWEGFSQSDFSRVMEIVHYFMVALIVG
                     WLSDRQRLQQKRLSQSERLAAVGQAMAGVAHDMKTPLIAIGGFTNLVKTKIRKVRLDE
                     ESFEVFAKDAEDKLLIVLKESRRLENMVKDMLDFSRPLELQKSKENVDELVSECVALV
                     SGQAEEKRVRVVAVQGNGFAPVSLDATRIKQALINLLMNAIQASPEGETVTVRSYRNG
                     RSLTIDVTDCGCGIPMNKREEIFSPFFTTKKEGTGLGLPLVIKIIEAHEGRLQIVDNP
                     EKGITFRVLVPIS"
     gene            complement(18752..>19370)
                     /locus_tag="PLM79_09655"
     CDS             complement(18752..>19370)
                     /locus_tag="PLM79_09655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOV86616.1"
                     /translation="LLVVADGLGGHASGERAAEIAVDALSGFDANSTALDRELTELVR
                     HANRNILQAAAEDLSHHGMGTTLTAAFISNGTAHWVHVGDSRLYLFRDQVVVQVTEDQ
                     TPVGLLLRTGELSPEEARVHPARHMLMSCLGRCELEVECGALDLQRGDLILLSTDGLH
                     DLMSEELIADILGSKRNLDDRMDDLVAAALAAGGRDNVTVAAAVI"
BASE COUNT         4243 a         5375 c         5290 g         4462 t
ORIGIN      
        1 gataccgtct catggccttg cggagcactc tttatcagtt catcaacgat catctgaatg
       61 ggccatggct gcacgagggt gagagccacc tggcccatca tagagatcgc cacgcctagt
      121 gtaaggcgcc aatgtgccct aagagccagc catagctgcg atcttgctgt tgtatggact
      181 gcactgcgga actgcttttt cataggccgg taatcgagta gacggctttc tacccaccat
      241 gcttagctgc ggcattttgc ccgcagtcaa gggaattgga ggctacgggt ttaggtggat
      301 ggtgataggc caggcgaaca gtcgtgccgg gctgcggtat gagtggaata cgagtctcaa
      361 agaccggctc ggggttacct cttctttgaa agtgggaatg ggagatcgat cgttggtcga
      421 tcagagtacc atcaggtgcg atgactacca tatccacatg gcctccatta gcaaatatag
      481 gaagcctgtt ggtcctcacg ctaccagcca ctatcatctc attgccttcc cgatatgcgt
      541 gggctcttga tatatactcc tttgttgaag gcagaatctc aaccgatacg gtgcctttat
      601 ccagcgttgt gcgatatgtt gcacaaccgg atggaaacat tgacaacagt aagacaaagc
      661 tcacggtaaa gatgcgtctt ggttgcgctc ccatctttac tccgcctccc ttcccaggtg
      721 taaaacacta taagtcgagc cttgggaaag gcccggctcg gcggttggga attgtctgga
      781 tgtaccctat ggactctcgt atgcctcttc catttagtct atctgtctgc cattgcaggg
      841 cctgccatat tcgtcgttgc agagtcctct ttagacgcct tcttttcttt cttcaccttt
      901 ccacgctgcc aacagggagg accagcatga agaggacagt agtggaagct gccatgagga
      961 ccgttcccgc cgcgtgcaaa cgccgttgaa ccggtgccca gaaagactgc gagtgccatt
     1021 accaacgcca ggatactctt tccgctagac atgttgcaga tctcctcaag tgattgttta
     1081 gcctctcccc ggttttcaaa gattcccggg ccatcatcac acacccaaaa tcacttatat
     1141 ctttaatccc ttgaatcggt tcccaggatc actgcattct gaccacaatc gaaagtcttt
     1201 gaaggtttgg actgaaagcg gtggtaggcg aaccgaaccg tcgtcccctt gggagcgacg
     1261 agctgaaacc gggccgtaaa tggagattga aggctcttca ccctcggcat gggaagaaac
     1321 ccggccgaag ccttatcgat cagcttgcca tccgatgtga aaacagccat atccatgtgc
     1381 gcatcgtagt ccctcggaag agaccaccgg cttttccttt tcacatatcc ggtaaccacc
     1441 aactcggtgc cgtcctgata aacgtcggtt tctttgagga aagccttctc gtcacccgca
     1501 gagatgatct caacggaagc cgtgcggttt tcaacaagat ttactcccgg tgtagcgcat
     1561 ccgtatgctg tgagcgcaac gatcagcagc acaaacatgg cagatccggt gaatcgtttc
     1621 cccatcactg ttatctcctt atgtacggtc aatggtgccg agccaatccg atgctgactt
     1681 ctgctatcca cttttgtgta aaaccgcggc gttttcaccg cagttgggag cgccatcggc
     1741 cagacgccga tcgctatggt aggcaaaacg caccacggtt cctcgttctg cgaaataggg
     1801 aaacctcacc ttgaaatcgg attgaacgcc ttccgctaat gatatcctga tggagcgctt
     1861 tcgcgctacc ttcctgtcgg gagccaaaac ggccacatcc acgtgggcat cgtagccggc
     1921 cggcaggaag tcagcggata tttcctcaac agatccggtg acgagcagac aatctccatc
     1981 ctgatagagc tcggtctcgg ttatggctgc atagcggtcc ctcgaggcaa ccagctctat
     2041 agagcccata ccggtatcga gaagatcgta gcctgtggtg catccggaag cggaaagcgc
     2101 caccaggtag ataaaaagac cggtcaatcc cttcgaaagc gtctttgtac tcatcgcttg
     2161 ccacccgtgt ccttaaatgg ccttttctcc gcgctcgccc gtgctgatgc gaacggcggc
     2221 cgtcacttcc gaaacataga tcttcccatc gcctcgaagc cccgtgtgcg cttccttctc
     2281 gatagttgaa acgatctgat cgaccagttc gtcccggcag acgatctcta tttttacgtg
     2341 ttgcacgtaa tcgatgaaat cctcggtgat cctctctttc accgtttttg ccctccctcg
     2401 tccgaatccc ttgacgttgg agatgctcat accggtaaga ccctcgattt tctggagggc
     2461 cagggtgacc tgggatagtt tgtgcggctt tatgtacgct tttatttctt tcatggatac
     2521 ttcctctcac tcgtccttgg gcaatgtatg caatttctct gctgaagggg tttccagctc
     2581 cactttgatg gcaaaccatt tatagatggc cggaatgacc agcagtgtga gaacggtcga
     2641 tgtgacgagc ccccctatga caaccgttgc cagggggcgc tggacttcgc ttcccgtccc
     2701 ggaggagaag agaagcggaa tgaggccgag catggcgcaa agggccgtca tcaaaacggg
     2761 ccgaagcctc aggcacgctc cgcgaatgga tgcctcatcg atgggaactc cgtcgctcac
     2821 gagctggttt atgtaggtga cgagcaccat accgtttgca agcacgatgc cgaatagggc
     2881 gataaagccc accgacgagg gtacggagag gttttggccc gttatccaga gggccgcaac
     2941 gcctcccacc agccccaggg ggatgttcag gaggatgagc agagtgttct tgagcgagtt
     3001 gaagctgctg aaaaggagca gaaagactat cagaagcgtt accggcacta cgagggccag
     3061 ccgtttgttg gcctcctgct ggagctcgaa ctgcccgccc caggtgacga tgtatcccgg
     3121 aggaagcttc acctcggcgc ttatggcctg ctgagcatcc gccacaaaag agcctatgtc
     3181 ccggccccgc acgttgcact gtacggcgat aaacctctgg ttgttctctc gggtgacctg
     3241 ccttggaccg acgatctcct cgatggtggt gagctcggcc aggggcacat ccacacccgc
     3301 aggcgatttc accacgatgc ggcgcatggc ctcaacggaa tcgcggctct ttggctcata
     3361 ccagaccacg atgtcgaagc ggcgcacgtc ctcgaagatc tgcccggcct tttcaccccc
     3421 gaccgccgtt ttgatcatgt tttgcaccgt ttcgacattg atcccgtagc gggcaagggc
     3481 cgggtagttc atggagatac gcacctgggg cgttcccgtg gtctggtcct tctgcacgtc
     3541 cgcagcccct ttcaccttcc ggaccacctg ctccacctcg cctgccttct ccttgagaac
     3601 ctccgtatcg gttccgaaaa tctttatggc gagctcggcc ttggttcccg tcaagagctc
     3661 atcgacggcg gccgcaatgg gctgcgtgaa gttgaactga acaccgggaa agttctcgaa
     3721 ggcctcgctc atcagcgcgt aaagatcttc gggtttctgt gtagtcgtcc actcaccgat
     3781 gggctttagg cccacgaagg cctcggcgct gtttacagga tcggcgtggg caccgacctc
     3841 gccccgcccg acacgggaga ccacccgcgt tacttcggga aacttttcca tgagctggcg
     3901 ctcgaagcga agcattgtgt ccctggcctc atcgagggat atggaagggg ccatggtagc
     3961 ccggaccagg agatctccct cattaaggcg cggcacgaac tccgagccaa gccgcgggaa
     4021 tatgagaagg cctgcggcaa ggatcaggac cgcaagcaga atggctgcaa aacgcatcct
     4081 cacgaaaaac cgtactacgg gctcatagag ctttagaagc attctgataa ggaatggctc
     4141 atggtgatgg tcccctctct tgccggcctt ggggcgccgc atgatgagat acgaagccac
     4201 gggagccaga aagatggcga agatgagaga gcccagcatc gcgatggaaa gcgtgtaggc
     4261 aaggggcttg aaggtctttc cctccactcc ctgcaagctg aagaggggca ggaaaacgat
     4321 gatgatgata aggatcgaaa agaagatggg acgccccacc tcggcgcaag cccgaacgac
     4381 gatgtgcagg cgcgattcgc tccggtctgc ctcgttcagc attcggtcca cgttttcaac
     4441 catcacgatg gtgccgtcca ccatcatgcc tatggcgatg gcaaggcctc ccatggacat
     4501 gaggttggcg gatatattga aagccttcat caggatgaag gtaaagagga tggagaaggg
     4561 aatggatacg gccaccacga ggctcggacg aatcccgcct agaaaagcca ggatcacgat
     4621 ggttacgagc acgatgcctt cgagcaaagc gtccttgacc gtctcgatgc acttggcaac
     4681 cagggtcgcc tggtcatagt agggcacaac cttgatccct ggcggcagca ccttgttgat
     4741 ctcatccatc cttgtcttgg ccgcctgtat gacatcggaa gtgttcgtgc cgatgagctt
     4801 taggaccatc cccacgaccg cctcgccttt gccgtccctc gtggccaggc cctggcgcag
     4861 ctcccccccg atcacaacgt cggccacgtc gtggagatga atgggggtgc cgtcaaccgt
     4921 tttcaagaca attcccttga ggtcggcgat gtccttggcg agccccacgg agcgcaccag
     4981 gtactgttca gcgtttttca cgatgtactg ggcgcccacg ttcgagttgt tggccttgac
     5041 cgcatcgacc acatcgccga tcgatagacc aaagcgcttg agctccgagg ggcggatctg
     5101 gacctggtac tgcttgacct cgccgccgag ggagaggatt tcggtaattc ccggaacggt
     5161 ctgcaggttg aactttatga gccagtcttg gatctcgcgc atctcctggg gggtgcgctt
     5221 tccggtctca tcttccacga aatagaagag gatctgcccg agccccgtcg agatcggccc
     5281 catctcgggc tctccgaacc cttcgtggat ctcttccttg gcaagctgca gccgctcgct
     5341 cacgagctgc cgtgcaaagt agatgtccgt tccatcctcg aagtatatgt ttatgaccga
     5401 gaggccgaag tttgagatgg aacggatctc cttgaggttg ggaagcccgt tcatggccac
     5461 ttcgaccggg taggtaacgt acttttcagc ctcttcgggg gcgagacctt cggtttcggt
     5521 aaagacctgc accaggacag gagtcacgtc cggaaaagca tccaccggca gatgcctgta
     5581 gcttgcatac cctgcaacga gcaccatggc tccgagcacg atcatgagca accggttgag
     5641 caaaacgaac tgaatgagct tgttcataga ggttccttaa aacaatgagc aggtgaatcg
     5701 gcaagcaggt aaaagggtct tccgattccc ccatacaccg gctcaccgat tcaccgctct
     5761 tattagtgat tgtggccatc accgaaggcc cccttggaga gctgtgcttt caaggtgaat
     5821 gttcccttgc tcacatactt ctccccggcg gaaaggcccg aggcgatctc gacgtcgccg
     5881 ttggtggccc gaccgaccac gaccggtctt gcctcgaagc cctcagccct ctgcacgaac
     5941 acgacctgct tttcttcgac ggtctgaaga gctgaactgg ggacaactat gggagcagat
     6001 gcgctgtcaa cagccacttt ggccgtaacg aacataccgg ggcgccagtt tccctgggga
     6061 ttggggagga ttacacgcgc acgggctgtt cgtgtctctt ccttgacgat aggctccaca
     6121 taggcgatct gcccttcggc ctcctggccg ccgccgtcct ggcccaggga gatgagaaca
     6181 cgctggccct tgcggatact ggcgatatct ttctggtaga tgtttatatt gacccatacg
     6241 gtggtcatgt cggccacgag aaagcattcg gcatcctctt tgagcatctc tccaagggag
     6301 atgtgctttt cgatgacagt cgaaggaaac ggggccatga tttcaaagcg ggtgagcgat
     6361 gagtcggcat gctgagggac ctgttttaag tgatcgcgcg aaagccccag ggcaaagagc
     6421 ttctgttcgg ccgatcgaag ggctatctgg gcctcggcca atgcctgttt tgcatccagg
     6481 tattcctgct gggaggagat cttctttttc caaagccctt cttccctgcg gtagctggcc
     6541 tgggccaaag aggtcttttc ctgtgcggca aggtattcag ccttggcgtc ggccagctcg
     6601 cggctctcga tcacggccat cacctctccc tgcttgaccg tatcgccgag cgtctttcga
     6661 acctccctta cgactccggc gatgcgtgga acgatgtgtg ccttacgatc ctcgttgaga
     6721 accacttcac ccggaaggga gatgaagttt tggagctccc ccgagccggc cgctgagacc
     6781 tcgatgccga actctttgat ctgcgcctca ctcaaccgga tggctttttc ttccacctcg
     6841 tcgccgtgac tatccttctg tgcgcctgca gccttttcct cgtcagcatg accgcccgct
     6901 tttgcggaag aagaggtatc ctccttgtgc ttgccttttg ccttctcccc accgtggcca
     6961 gcatgggcat cggcttgctc ttcggggtga gcgctctcgg cccgggcttt cgccccgggc
     7021 tcatcatgtc ctgcatgcga gcctgctttc tccccctggg ccgcgagcag gaggcccgaa
     7081 gggccgtagc ttcgaaaagc cagaacggcg agcatcactc ccgcaacggc aagcgcaatc
     7141 catagggctg actttatctt cattgagttt ctcctctatt tgcttggtgt ttccatggcg
     7201 gcatcgagct tttctccggt aagacgttcc acgtcggctg ccgctttatg gtatgtggca
     7261 agggtttcga tgtattttcc cctggcctcg aaaagggtcc gctgggagtc gaggaattcc
     7321 aggaaattga attttccctc ccggtagccc tcaccggatg cctggaaagc gagctcggct
     7381 gccggcagca cctcgccctt caggctcacg gccccttcat aggaagcgga aagctcctgg
     7441 taggcatcgg caagacccgt gttggccttt gcaaccgccg ccctagattc ctctttggcc
     7501 ttggccagtt tgtgctgcgc ttccttgatt cctccctgat tgcggtcaaa aaggggaagg
     7561 ggaagggaga cgccgatgac aaaggcatgg tcgtccgtat cgttgaaccg ctggacgccg
     7621 ccgctcacgg ttagatccgg gatgcgcctg gccttttcga cctcgagggc cgccgtgcgc
     7681 tgctccatct cctttaccca ccgggccacg tccgggtttt tcgaaatgag attcgtcaac
     7741 tgctcccgtg aggggatggc ttttacagta tccagctcgc catctgcttt ctcaaacgtt
     7801 ggctcatcct gcccccaaat gctggagagt ctcttgcgag accctttgag actgcttttg
     7861 gccttttcca gatcgatccg ggtattggaa agctcgaccc tggacttgac ctcttcaaga
     7921 ggggagacct tcccggcatc gacgcgctcg gagaccgagt agacgacctg ctcggcgagg
     7981 cggacgagct cttctgcaag agctacctgc tcctgggctg ccaccacatc gataaaggcc
     8041 ttggtagtgc cgatcaggac atcgagcctt ttggcctggt agtcccagcc ggcaaggtcc
     8101 tgctcgaggg ccgccgattg agtcctcttg cttcgttttc ctccaagctc gatcaactgg
     8161 ctgagctgga atgtggtcgt tgccacgtca aatccactcc ggtcgcctcc tcccgcaaat
     8221 tcttcaacct caatgcccgc ctccgggttg ggtaaaagac ttgcctggag cttgcgcgcc
     8281 tcggccgcgc ggatgtccca ggaggtgccc gcaagctccg ggtttttcat aagggcatgc
     8341 gccagggcgt ctcttagcgt gagcactccg gtgggttcgc ccgcaggcac ggtgtccatg
     8401 ccgggctcag cggacttttg cggaattgca ggtgttttga ggtcactttc cccaatgtcc
     8461 tgctccggtt ttgccaggcc ggtaactctt ttttgagcgc atcccgctgt agcgatcagc
     8521 agcaagacac agcccagaac cgaaactcct ctcatgtaca ttaagcgcat ggaaatcctc
     8581 cccctttttt gaattgagat ggtcagttgt ctcacctggt gccaacactt gagagaatat
     8641 aaggacttat agtccacact tcaggtgaga tgcatcgacg gacatgaaaa aaatggagga
     8701 tttggcttaa atcaggagca cgacggatga gaggcgagct agaacaaggc ctgatgtgac
     8761 aggctgttgc ccggcaaaaa ggcttgtgcc tggtgtttcg gcacagtcgg cgataagaac
     8821 gggtaaggaa tcggcaaaga gcggctgctg ctctattatt ggattgggcg cggaagggta
     8881 aagagcgctg gtaatcgata gggcaatatc ggtgcagggg ccgcaatcat ttccggcaga
     8941 gggagaggcg ggagaagaat tctctaaaaa ggaaaatgcg gctttctccg gcacctggtt
     9001 gcagcctgca tcccggagga attcgagccg tagatggtca ttggctccga agcacagaaa
     9061 cgcactttcc gcgcttccga ccaccagcca caaagacaag gccaggctga accataaggt
     9121 gaatcttctc tgtcgtccga ttttgaaaaa cttcacgctt tccatcccac tccatccagg
     9181 caattgattg cataataaat caccaaattc gcacaacttg tcaagatacc cggcaaaaaa
     9241 gagcgcactg ccgtgagctt ccgtttatgg aacagcaggc cgcatcgctt cgccaaacag
     9301 tttagcaatc tctataccag ggcatcgctt cagggtgttc agtccgctgt ccgatcagat
     9361 tttgaagagt aggatcaatg tcttgtgtcg ctccgcaact ctcaatcctc ggcaaagagc
     9421 tcctgcttgt ttcgtctaga ggagcttggg gaccgaatca acactgaaga gagagcccag
     9481 gtattgaaag aatctttcaa tacctggacg gcgctcatga gagaatgcgg tttggtcaca
     9541 taaaaacgga gagaaaggag tgtcggaatt gctctggcgt gcagtaggag ggaaaaagaa
     9601 gggggtatgt gattcatacc ccctatgcaa cccgcaatac gtttattgct ttccactagt
     9661 ttgtttgtct gcttaccatt tgtagggagg ataggcctta gtcggaaccc cgctcggttc
     9721 gcactgggtt cctgcggtag tagcaggtgc cgccatactg cgtgtgctgc tctgcgctgc
     9781 ggcactttta cctgtcgcat ccgtggaagc acagcccgtg aggctaaaca tcaacactat
     9841 caacagcagt cctatagcct ttaacatatt cttatccttt catataagct ttgacaggtc
     9901 caatctgttc aaaatgattc ctgccagcat tcttaggaga tgcgtctcac cagttatcag
     9961 cagtgtttct agcgccccag acgaccttgc ccgccatgcc cgtgtccacc gccaccatgc
    10021 cctccaccat gaccacctcc gccattacgc gccgctgtgg tatactcatc catgcttccc
    10081 gttgccgccc acagggatgc ggtgctggca gtgagggtga ttgccagaac tgctacggct
    10141 agaatctttt tgaacatagt tcgcctccgg tttggattta attgttttta cgagaaaata
    10201 tccaggctcg caaggtgccc catgcaacac tcgtgccgca ggcgcactca gggtgcacga
    10261 tactgataac tggctggaaa cattggtttc taaggacatc tcttgccagg tcctcagaga
    10321 gcttcccgga tgggtcggag aaaagaaaaa gaatttacac gggcatcgaa agggagtttc
    10381 aattatccac ggagaaggga aacgatcatg agaggctttt gaaaatacct ttcaattttc
    10441 agccgggaaa gtggttgggt tcgcaagaag cgggctgagg agtcagtctt tttcaattcc
    10501 ttggcatcgg ctcggggtag aaggaacttg cggcgcgcat cctcttgctt ttccgtcggc
    10561 tggtcggtca caatgacaag ggattccaat taaccgcatg caccaggaga gaaccgtgtt
    10621 tctcattaaa aagaggcttt cccacagatt ttttgtggca ttgatgctca tttccctaat
    10681 tcccataggg ctcatggggg ttcaggctca cgcgctcgcc aggagggctc ttacggcatc
    10741 ggcctttctg cacatggaaa ctatagctaa ggaccacgct agacatctcg atgcatggct
    10801 caaggagcgg ctgggcgata tcggcgtgct cgcaaggctt cccgcaatga gggaagcctt
    10861 gcgggagtat tgctgcactc ttgactccgt agcaccatcc ggagaactaa ccgcgttgct
    10921 caaggatacc atcaccgcaa cccgggaaag gtcctcttcc tatgagagca tctacgtcat
    10981 gctgccgggc gggggaatca ttgccgcaac gcatccccct tccgaagatt tctttgagtt
    11041 gcagcgtgac ggattcatcc agcgcgtcat ggatgggagc gagcccgcct tcggttctct
    11101 gtgcagggga gccgattcaa agtggcatat gcctgtgggc gcaaagatac gggatcggga
    11161 tggcactacc cttgccttgg tagttgccgt actcgatgtg tcccaaacca tcgatcctgt
    11221 catgaacgat cgaatcggtc tgggagaaac cggagaaacc tatttggtcg gcaaagaagg
    11281 aaagatcata tctgaatccc gtttcctgag ccagtccgag accacgaaac agaccttcga
    11341 cacattagga atacgtgcgg tgttggaagg aaggcaagga acggcgattt acagcaacta
    11401 tctgggacgc gaggtcttgg gctcctacct gtggcttccc cgctacgagt ggggcatact
    11461 ggccgagatg gaagccgatg agatcctgtg gcccctcaag tggatcaagg cagcgagcat
    11521 cctcaccgcc cttttggtga gctcggcatg tttgctcatg gcctaccttg tcagcaggcg
    11581 tattgcaaaa cccatcagtg atgtcgcaga tgccgcacaa aggatgagcg aaggcgatct
    11641 cgatcagcgc atcaccttta gcagccagga tgaagtgggc atgctggccg gaagcttcaa
    11701 caccatggct cagcaggtct cacggctcat tcactccctg caccaaaagg aggaatccct
    11761 gcagaaggcc tacgatgagc ttgtggccac acagaaccaa ctgatccagt ccgagagaat
    11821 ggcggctata ggcgagctgg tggcctgcat tgcccatgag atgaggaacc ctctctcgtc
    11881 ggtcaagctc aatctgcaga ttatggagat ggaccttaaa gaggacgacg ttctctccga
    11941 acattgcagt atcgctctcg atcaggtggg acagcttgag gggatgtttt cggatcttct
    12001 caattacagc aagcctctgg tgctggaaaa gagatcggtt gtggtagagg agctcgtcaa
    12061 cagaggcctc gagcaattga gtggtgagat agcttccagg gacattcgtg ttataaggac
    12121 gaatcacatg gacctcctgg ccgtcacagc ggatgcggac aaaatcgtcc aggtgctggt
    12181 caacgttcta aaaaacgcca tgcaggcatc accgcagggt agtgaaatca gaataaccat
    12241 acaagaggac gaggctccac aaggaagagt ctcagaaatc attattacag atcatggtcc
    12301 gggaatttcg gcacggaatt tgcaaaatat atttcaaccc ttctttacga caaaaaagaa
    12361 aggcaccggt ttgggccttt gcatcgtgaa aaagatcatg gacgctcacc gtgggacagt
    12421 ctccgtttca agcgaggagg gcaaaggaac cacagtgcgc ctggtcctgc cggttgcctg
    12481 aggttacggg atgagaaaaa ttctaatcat cgatgacgac ccgtcacttg ccagaaccct
    12541 cgagctctac tttcgaagga agaagtgtga ggtttccacc tcaggaaaag gaaaggaagc
    12601 gcttgagctg tgggcatccg aagagccgga tctcatcctg ctcgatgtgc agcttcccga
    12661 catggacggg ccggatgtgc tggcgatggc caaaaaggaa cagctcaagg gcgaggtcat
    12721 catgatcacg gcctttcaca atacggaggc caccttgaag gccatccgcc ttggcgctat
    12781 cgactacctc tacaagccca tagaccgcca ggcgctggat cttttgctcg aaaaaacggc
    12841 cgttcagaaa aagcagcggg agcgggctgc gaagctctgt catgtcatct cggaatccta
    12901 caggtcagag cagatcatcg ggagaagctc gggctcactg gaggtcataa aggctatcgc
    12961 ccaagtctcc cagactccga ctaacgttct catcgaaggg gagaccggga cgggaaagga
    13021 gctcgtagcc cgaaccattc accaggaatc ggcccccgag gagcccttcg tcgccataaa
    13081 ctgtgcggcg atcgtgggga gcctcttgga aagcgagctc ttcggccatg aaaagggggc
    13141 ctttacggga gccgtgcaga gaaaggtcgg caaactggag tgcgccggcg aggggacggt
    13201 ctttctggac gaggtcggcg agctccccct ggaatttcag gtaaagttct tgcgggttct
    13261 ccaggagcgg gagtttcagc gagttggcgg aaataagacg atacctctgc aggcacgcat
    13321 cattgcggcc accaaccgcg acctccacga aatggtacaa gagggggctt ttcgcgaaga
    13381 cctctacttt cgcctcaaag tctttgtcat ccgtattcct ccactgcggg agaggaccga
    13441 ggatatcgtt cccttgaccg aatacttcct gacccgcctc aaccaggaga tgcacaaaag
    13501 ggtcgtgcgc attcccaatg cctacctgga ggcgttgaga acctatgggt ggccgggaaa
    13561 catacgggag cttcaaaacg tgcttcgccg cgggctcatc ctgtctcggg gagaggtcct
    13621 cgagctcaat gagacctggc tcacggccgg agagacgccc cttgaagccg aagggaggcg
    13681 ggaggtgccc caaagcctgg cagacatgga aaaggctcat attcttaaag tcttggagta
    13741 cacggactgg aactatgggg aggcctgcca gattcttggc atcagccggc ccacgctgcg
    13801 gaaaaagatt gccgattatg ggctgagctc cgaaggcgat gagaatggca tggtgtgatc
    13861 tcttcccgtg cgggaggtgg aaagatgcgt atcgtactcg tcgaaaatga cccggtcttg
    13921 ctgaaaaccc tggagatgct gctcaggagg cagggctgtc aggtcgtggc cttccatgag
    13981 cctgccatgg cttgcgcctt cctggagcgg ggcgagcact tcgatcttct catcatcgac
    14041 tacatgatgc ccgagctcac cggccgcgag gtgctggagc gggtgcgagg tcgtttcccc
    14101 aagggctgca gagtggttct catcagcgga cacacggatg tcgtcgagtc tctggacctc
    14161 aaagccatgg gcgtggacac cttcctgccc aagccgctcg atttgaagag actgcgacaa
    14221 accattagta tatcctctgg ataagctggc cagacatgct caaatgacga aggggatgtg
    14281 gattccatac cacatcccct tctctttcag cgcacaagcc gagtacggct aatttgccgt
    14341 tccccttctt ttagtgcgaa catactgcac ggccaagggg attgttgttt gggtcgtaac
    14401 acgcggcctt gatggtctgg ccgttggcat cgtaggtgta gctgcaggag ccgatctgaa
    14461 cgccgtcggc attgtgttcc agaactttgt taagctgtcc gagtccgtta tgctcgtagg
    14521 atttggattc tataactctt ccggcagggt cgcgatgcat ggttttcgtc aagtgaccgt
    14581 tggcgttgta ctggcaggtt ctgtaccatt gaagcgcgcc tcccgcatca tactgagata
    14641 ccttgaccaa ccggccgagc gaattgtagc tgcacttggt tgtatacgac agggacccat
    14701 gctcataggt gcgcgccgtc acaagcttgc cggcattgtt gtaaacgtat tgggtgtaac
    14761 ctgtctgagc gtcggccatg tcatagttga gcgttcttac aagcagtccg ctgctgttat
    14821 accggcaaat agtgtatccc tgcaaacctt cgaatgagct atggtgttct attctggaaa
    14881 tacgacctgc tgcgtcataa gccatggtgt aataatagtc agggtttcct tgcgcatcgt
    14941 agtgaatctc cttggttgtt ccagcgtgct ccgtttccct gttgaaatgg ctgctcaaac
    15001 agtcaccggg aatagccgga tgaaggaggt gttttacgat gtcctcaggg tgtgaagctt
    15061 gggagtgatg gtcaaaggta tggtgggttg tctgaccgca ggtggaacac ggggtttcag
    15121 tacctccatg ctcgccttcg tgtccgcctc cgtcatggct tgattcatat gcgaagacgg
    15181 gcgaaaagca gaggaatgta gcaaagacca gcaatccgaa tgtgaagtga ggtgccttgg
    15241 atttcatgta ttgtaatact cctatagaat agtgaactga atagaattcc tctttctaac
    15301 gacacacgct agcctgttgg agcgcctatg agaggcgctc caacctgtga ttactttacc
    15361 cggggggcta tcgacttcca tctgggtagt cgggagaaac aggctctcct tcaggcctcg
    15421 gcagatagcc gaggcccttg attatccttt ggagatagaa gaggaaagaa gcccctgcag
    15481 gctcctagcg atgtcctcca ccatgaccgc ccccgtggcc gccaccatga ccgcctctgt
    15541 ggccgcgatg ctccaccgtg gtgtactgat ccatgcttcc catggccgcc cacaggggcg
    15601 cactgctcac tgtgagggtg attgcgagaa cggctgctgc cagaatcctt ttgaacatgg
    15661 tcgttgcctc caatgaaaag ttgtgggtaa tgagttgtgc agcgcgactg cgccgcgatc
    15721 aatcggactg cacaatatgc aatgggtgtg ccgtgccggt tcgcttggtc ggaatcggaa
    15781 gctaactctt gtgaatcata cggtatccgc tcaaggctcc cgtttgggcc aaagcaggta
    15841 tagtggtgtt tcgctgaaag gaagagaggt ttttccctga tgagctgcaa taaactttca
    15901 acaagagagc gaaatcactt ttcaactcga aaatagttga aagaatgctt catttcttga
    15961 ggtcttatgt ggagtttccg gttgctcgga tctcggcggc aaaatgaccc gtcggtcgca
    16021 tggagatcac gtggtcttgt tcgggtctca gcgtgatggt aatggtacga aaatacccca
    16081 tccaccttct cggggagtac atagcgggag gcgcatgcag cgcaggcagc atcattccgg
    16141 acggcttcga cgttcctcca tccgtttcgc ttgatccgct tgcttgctta ttcaaacatc
    16201 gccgatgtca ggccgactcc tatcactttg cctggggtct caatctgctt tgcagcaatc
    16261 cgaagccaag cgcattccct aatcgaacac atatttggaa gtctcacttg agaatcttct
    16321 cgaaaaagct ctccacagcg tttgggaggc gcttatcctc acccctgcca taattggggt
    16381 cggtaaagac actgaaagcc tcggcaaagc cttctttctg attgaccgtt gcgtacttgg
    16441 aaatggaaac tgacactggg ctttcggaag acatccgccc cttgctctca gtgagctcac
    16501 ctccgacaga ctcatataca gaatcccact cctcgctcag ctctttcggc aagaaatcgt
    16561 gaaccatatg accgaactca tggcgggttg tgcccaagcc gcatgaggtg tacgcgaact
    16621 cgttgcccag acgatactgc tctatccgga aaaaatccag gagccccgcc gctatgtgaa
    16681 tagagccaca atcatagcaa cagcgggctg ctccgtcata gacgtcacct gggaggtttt
    16741 caccggcgta gatgaatagc ttgcgaggga aatggggatt gggctcttta tctctattgg
    16801 cctggatgat ctctacgccg ggaaaatcat tgcgcaccct ggtccaatcc cttccgatag
    16861 catccatgat gtccttgaaa cgctctttgc tcaaggcacg agacctctcc aggccattaa
    16921 acccaaactc tcgcgccttc ctctcatacc aatccatatc aagcttgttg ggagggtccc
    16981 acgtgagctt agtgacgggc ttgctgggct ccggtgattg cattggaaca gcttccgaaa
    17041 ccctcggtac cgactgagta ttagtgcttg gtggttccga aagcttcatc gtcttctcag
    17101 cggtaacatt cgccacaggg ctgattttag ccgggagccc cagccaagtg ccctttatga
    17161 ccacgcttgg ggtcaacttc atggggccgt tgtccagctc aaccttctca tggccgctgg
    17221 tagtgcggtc gcggatatgt ccgccactgt ccacgatgta gaactcgtag gttttccgcc
    17281 aacccgaacc ttgcatttct tcggcagcct catggtgcat ctctaccgat gggtttttcg
    17341 ggcgctggct catgtcccta gaatgggcag tgggagtaag cttgagaaat gtcgctttgc
    17401 gacactcctt gaacatgttc tccgaagcgt agaccaaaac ggtggttccg atgccgatca
    17461 tgatgacggc gggaggcacc aggaaaaaga aaaccacacg ctgctgcagg cctttaattc
    17521 tgaacatggt cacattcccg atcgtcgacc gccgtttgta gtgcgagaat cgtgtcgagt
    17581 ggcattgacc ggccctcccc atttgtcctg gcactggatc aggatatcgg gacaaggacg
    17641 cgaaaggtaa tccccttttc agggttgtcg acgatctgca atcgaccctc atgagcttcg
    17701 atgatcttta tgacgagggg aagcccgagg ccggtacctt cctttttggt cgtaaagaaa
    17761 gggctgaaga tttcctctct cttgttcatc gggatgccgc aaccgcaatc cgttacgtcg
    17821 atagtcagac ttcttccgtt acgatagctg cgaaccgtca cggtttctcc ttccggcgag
    17881 gcctggattg cgttcatcag aagattgatg agggcctgct tgattctcgt ggcatccaaa
    17941 gagaccggtg cgaagccgtt tccctgtacg gccacaaccc gcactctttt ttcttccgcc
    18001 tggcctgaga ccaacgccac gcactccgat accaattcat ccacgttctc cttggacttt
    18061 tgcagctcca gggggcgaga aaaatcgagc atgtccttca ccatattttc aaggcggcga
    18121 gattccttga ggacgatcag aagcttgtcc tcagcgtctt tagcaaagac ctcgaagctt
    18181 tcctcatcga gccgaacctt tcggattttt gtcttcacca aattggtgaa gccgccgatg
    18241 gcaatcaaag gggttttcat atcatgggcc acaccggcca tcgcctgccc gacggcggcg
    18301 agcctttcag actggctcaa gcgcttttgc tgaagcctct gtcgatccga cagccacccg
    18361 acgatcaggg ctaccatgaa gtaatgaaca atctccataa cacgactgaa atcactctga
    18421 gaaaatccct cccagtagat caggatgaac ggagtataga gcagagtgat cccgacactg
    18481 cccgccagtg ccccgcgcag accgaaccag aatgcggcaa gtatcaaagg tatgtagtaa
    18541 agcccctggt agatgatgtg gagatgatgg ctctgtattc ctgtggcgta atgaagaacc
    18601 gtcacagccg caagcagggc tgacacgaga atgacctttt ccttttgtct gatttcaatc
    18661 acggattcaa acctcctccc gggcttggga gcaaatcccg gcggctgcca gccaggctcg
    18721 cacgagacgg ccttgggagt catagaactt ttcatatcac tgccgcagcg actgtgacgt
    18781 tgtcccttcc tccggcggcc agggccgccg cgacaagatc gtccattctg tcatcgaggt
    18841 tcctcttact tccgaggatg tccgcaatga gctcttcact catcaggtcg tgaagcccgt
    18901 cggtagagag caggatcaga tccccccgct ggagatcgag tgctccgcat tccacctcga
    18961 gctcacagcg cccgaggcag ctcatgagca tgtgtcgggc cggatggacc cttgcctctt
    19021 cagggctgag ctcgcccgtt ctcaggagca gtcctacagg ggtctgatct tccgttacct
    19081 gaacaactac ctggtcacgg aaaaggtaga gtctactgtc tcccacatgg acccaatgag
    19141 ctgttccatt gctgataaag gcagccgtca atgtggttcc catgccgtgg tgggagagat
    19201 cttccgccgc agcctgcagt atgtttcggt tggcatgcct gaccaattca gtcagctccc
    19261 tgtccagggc tgtcgaattc gcgtcgaagc cactgagcgc atcaacggca atctcggctg
    19321 cccgctctcc cgaggcatgg ccgcccaatc cgtctgcaac caccaagagg
//
LOCUS       DAOOVC010000063        19106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1963_length_19106_cov_6.805994, whole genome shotgun sequence.
ACCESSION   DAOOVC010000063 DAOOVC010000000
VERSION     DAOOVC010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19106
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_19106_cov_6.805994"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1390
                     /gene="ftsW"
                     /locus_tag="PLM79_09660"
     CDS             185..1390
                     /gene="ftsW"
                     /locus_tag="PLM79_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOV86617.1"
                     /translation="MLRPSADTSAFGMEPAARETESIPASLPLELHLWIVVSFLVLLG
                     LIMVYSASSTAPFKELAERAALLKSPSHYLKRQLICIALGIGIIVLVRRIPHGWYKEQ
                     ARWMFLLSIAALMLVLIPGVGAEFNHARRWFQWRGFSLQPAEYAKVIWVLFLSVSLVK
                     KQDRIRQVWIGFLPHVLLCGILSVLVLLEPDFGNTFVVGCITVLLLAVGGVPFGQLLT
                     LVPVAVLGFYKFVYLVPYRWERVTAFRNPWTDPLDSGYQLIQAWIAVGSGGLLGKGLG
                     AGQQKLFYLPESHTDFILAVIGEELGFAGILAVCALFFFLFRAGMQISRRAPDLLGSL
                     IAFGLTMLLSIQGLFNMGVVLGLVPTKGLPLPFVSYGGSAFLANCAAVGVLMSIARSS
                     ENRREPSYV"
     gene            1383..2771
                     /gene="murC"
                     /locus_tag="PLM79_09665"
     CDS             1383..2771
                     /gene="murC"
                     /locus_tag="PLM79_09665"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOV86618.1"
                     /translation="MFKRYQHIHFVGIGGIGMSGIAELLLNLGYRVSGSDLKETDLTR
                     RLGALGARIFVGHAAEHVRGADVVVLSSAISEDNPEWLAARALGKVPIIRRAEMLAEL
                     MRLKYSVLIAGAHGKTTTTSMVSTVLARGGLDPTVVIGGKLNAWGTNAKLGHGDFMVA
                     EADESDGTFLMLPPTIAVVTNIDLEHLDFYRDLEHIQETFLQFINKIPFYGQAVLCLE
                     DENIQALMPRIGKRFVTYGFSSQADFQARDVRIDGFRASYRAYCRGEELGEIELGIPG
                     RHNVLNSLAAVAVARELEIAWPDVQTGLGDMTGVQRRFQVKGEVDGVLVLDDYGHHPT
                     EIRTVLETLAQCFPNRRRIVAFQPHRYSRTHALREQFARCFYHSDVLLTTEIYAASEK
                     PIEGVTGEGLAREIASHGHHDLHYCRTLEEMLEKLCEMVVPGDVVMTLGAGNILQVGE
                     WLLDRLEQRRRA"
     gene            2842..3750
                     /gene="murB"
                     /locus_tag="PLM79_09670"
     CDS             2842..3750
                     /gene="murB"
                     /locus_tag="PLM79_09670"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOV86619.1"
                     /translation="MREFKALFRSLDVEAEWLEPMSRHTTFRIGGPVACLVRPLDEAS
                     LAKALGELKRLGLPHFILGAGSNILPPDDPWDVAAVQLTRSCSDIVPIEEHGDRVRVR
                     VGAGGMLSRLLRFCVRHQLGGIEFLAGIPGTVGGAVVMNAGTKRGCIGEALLWVDVLD
                     GNGRRHRLEKSRLSVSYRSLGLPPDAVVLGACLDLRRSVGAKLRGELLEILRHRRATQ
                     PLGYPSAGCIFKNPPGASAGALIEQVGLKGWKVGGAQISEKHANWIVNLGKARAEEVL
                     ALVEEAEKRVFEAFGLRMEREIRILG"
     gene            3747..4577
                     /locus_tag="PLM79_09675"
     CDS             3747..4577
                     /locus_tag="PLM79_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOV86620.1"
                     /translation="MIKKKKNRYRSDSRKARKAVATFLKAIGAVVLSLAGTLLLSAGL
                     VRGYHAALDAPLWRVEEIRITGNRHLERRDILNVLGVEKGACILNVKTSQLRRRLCAM
                     PWVRHAEVQLDFPGSILVRIEERTPLAIVHTEDLFLMDEEGRLFVRDRLENHPGLLYV
                     TGFSALGLKEGDFLPREPLQQLKGLLGALAQIRGSLPPQLISECHWRGEEGFTIYTAP
                     SAVPVQLGSQEFDLKIERLQMVLRVLAEREWLNLVTRIDLNYPDRAYVEGRFPTPKGT
                     "
     gene            4723..5946
                     /gene="ftsA"
                     /locus_tag="PLM79_09680"
     CDS             4723..5946
                     /gene="ftsA"
                     /locus_tag="PLM79_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOV86621.1"
                     /translation="MGKREELIVGLDLGTTKICAVVGELTSEGIDIVGVGSYPSVGLR
                     AGVVVNIDQTVNAIRKAVEEAELMAGCEISSVYAGVAGTHVQSLNSHGVIAIKSREVN
                     QTDIDRVLDAAKTVALPFDRQILHVLPQQYIVDDQEGIHNPLGMAGVRLEAKVHIITG
                     VTSAVQNIVKCCERAGLQVQDVVLESLAAAESVLDPDEKHLGVALVDMGGGTSDVAIF
                     SDHAIRYSCVVGLGGNHITSDISVGLRTSMDEAEKIKKKFGCALSEHVGPQDVIEVGS
                     VGGQKPRQLAKSILTQIIEARVEEIMKIIEWELVRSGYVESLHAGVVLTGGVSLLPGI
                     RELAERALDMPVRIGVPYRFGGLGDVVKNPIYSTASGLLLYGRKHGSRGGRLEEPSLF
                     KKVMESVRRWFREFW"
     gene            6071..7273
                     /gene="ftsZ"
                     /locus_tag="PLM79_09685"
     CDS             6071..7273
                     /gene="ftsZ"
                     /locus_tag="PLM79_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOV86622.1"
                     /translation="MENSALNRAKIKVLGIGGGGGNAINNMISEGLEGVEFVAANTDF
                     QVLERSLAPAKIQLGVNLTKGLGAGGNPEVGGKATQEDVDKIRAAVDGSDMVFITAGL
                     GGGTGTGGAPIAAQVCKEMGILTVAVVTKPFAFEGKVRHRNADEGLKSLQGVVDTLIT
                     IPNDRLLCLADRRATFLEMFKRADDVLLYAVKGISDLIVNPGYINVDFADVKTVMGEM
                     GLALMGTGSGRGENRAAQAAQQAISSPLLEDISIHGARAALINICAGPDLGMHEFEEA
                     SSIIQKEVDDEAHIILGMAIDPAMEDEIRVTVIATGIGKVEKSEPVSTAAARAKRAKV
                     EGIIPPELNYDYLQIPTVVRHRPVRDNGPGAGSEPPKRRTFLQRLTGAVSEEEDLNIP
                     TFIRRQAD"
     gene            7771..9522
                     /locus_tag="PLM79_09690"
     CDS             7771..9522
                     /locus_tag="PLM79_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86623.1"
                     /translation="MHWKWKQRHRQRLSAERGTIRKDWRGKVRIALAFPNRYAVAMSN
                     LGFQSVYAALNEVDHVVCERVFYPETADLPHVKQHPGSLLSLESQRPVREFDLVAFSV
                     PYENDYSHVVEMLSLAGIEPRAARRSRESPVVAAGGVAVILNPEPLSPFMDFFFIGEA
                     EGMLPDLEGRWGEFPERRDDRPDFLHQLAREVSGIYVPSLYTDSYLADGTLRSLDPVH
                     KDLPEKVPYGRGNLDRAKPCRTVVLTPETEFSDVTLLEIGRGCGRGCRFCAAGYVYRP
                     VRFHAGSRVVDAAAERLGESPRVGLVSAAVSDHPEIDFICRSLLDRGASLSFSSLRAD
                     GITPEIASTLEAGRHQAAALAPEAGSDRLRKLINKNLTVEQVHRAVKELVDKGVMHLK
                     LYFMIGLPTETAEDLEAVVDLVKGIRHEILKSSRGRTRLGTVTLSVHSFVPKPFTPFQ
                     WAPFAGVRELKDRGKRLQRTLQKVANVRVHFDLPKWAYVQALLSRGDRRVASILEKVA
                     LEGVGWTQAMRSAPANPDFWVMRERGKEEIFPWEIVDLGLKKSFLWEEYRRALRGEPS
                     PPCRPHENCRRCGVCAP"
     gene            complement(9996..10433)
                     /locus_tag="PLM79_09695"
     CDS             complement(9996..10433)
                     /locus_tag="PLM79_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86624.1"
                     /translation="MKKLSLGVALMVLLLTAAVGVAQETEDPAAIWVSVPRVEGTWTG
                     KWFSYFGGSGRIKAVVKQSGTVLKGTLRVTNTHCGTVSTNLNGSVSNTTAKFKAVFDC
                     REDKMVLKFTKGKLSSGGTKITGTYHVFVNDSLADYGTFSANR"
     gene            11100..14255
                     /locus_tag="PLM79_09700"
     CDS             11100..14255
                     /locus_tag="PLM79_09700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV86625.1"
                     /translation="MIVLHSAIGEAGLYLWGESPSEGQAGPRRRPGRKPKAPSTKPLP
                     YDAGPEALGEALSEGGMAAGKTGIEPLKGFVWIPSAGSIPAASSPLIAEPPGVEGKVQ
                     VSPWVVSILALDGSRSFEFLCRCVGKTILSPGTILGDDLLFLTKAMQFAAALVAKQQF
                     LPGVARVGRSYFARWKPVFLGADGERLGRLAGAMPSACRAVQFQADSPPETPSLTLLS
                     NFVGGMVDHLVRFSAAPGPFEPLPSRGRKKAAVFDSIHDQWLHALGTPDGKMDGSEAE
                     LARFALQVEDWHRPVTTSAASAFRLCFRLDEPGPAVEAASTGRRGGASPQDGEGWTVH
                     YLLQAADDPSLLVPAEHAWAARGRRASVLKRENFNPREYLLCALGQASGLCPNIEASL
                     KGPVPRGYRLDTSGAYDFLTQRAWALEQAGFGVLLPAWWTRKGTKLKLTARAGVKSPR
                     MQAKAGLGLDEIVQFNWELALGGEPLTFKELQELARLKSPLVRVRGQWVELNPEEIRA
                     AVDFWKKQAGGETTAREVVKMALGVHGAPGSIPFGGVRATGWIADVLSQLEGRTPFEE
                     LPVPRRFRGTLRPYQVRGYSWLGFLRRFGLGACLADDMGLGKTVQALSLIQKDWESNG
                     GKPVLLISPMSVVNNWRKEARRFTPDLPVLVHHGAARSKGKTFKSQAGKHALVLSSYS
                     LLYRDFETLKSVPWAGIVLDEAQNIKNPEAKQAKAVRALEAGYRIALTGTPVENNVGD
                     LWSIMEFLNPGFLGSQSEFRRKFFIPIQANRDPEATERLKRITGPFVLRRLKTDKAVI
                     ADLPDKLEMKVYCTLTKEQASLYEAVLKDLGEALEASEGIQRKGIVLATLSKLKQVCN
                     HPAQFLGDNSAIPGRSGKLARLTEMIEEILHVGDRTLVFTQFTEMGAILRRHLQETFG
                     REVLFLHGGIPKKQRDHMVERFQGVENGPGIFILSLKAGGTGLNLTRAAHVFHFDRWW
                     NPAVENQATDRAFRIGQTKNVQVHKFICAGTLEERIDEMIEKKKEISEGVVGSGEAWL
                     TELSTTELKKLFALGKDAAAE"
     gene            14348..15250
                     /locus_tag="PLM79_09705"
     CDS             14348..15250
                     /locus_tag="PLM79_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HOV86626.1"
                     /translation="MSWRYYDHFPRSTPKAAKGGIKAQSKRGGFGNSWWAKRWIAVLE
                     SFNIGERLGRGRSYARRGQVLSIDIEKGKVKARVQGSRPRPYEVALQVKVLSPADWRK
                     LAQALSSRILFVAKLLAGEMPQDIEQVFDGAGLSLFPSKSKDLATSCSCPDWSNPCKH
                     IAAVYYLLGEEFDRDPFLIFKLRGMEREELLAGLAQTGKGGSRKEFGGMECPGATEDA
                     APPLDPICADGEVFWKGSRLPEDLLGEVRIPPLRAALLKRLGGFPFWRGNRLIQEALE
                     PVYSNASQRGMDIFLYESERGPSR"
     gene            complement(15338..15604)
                     /locus_tag="PLM79_09710"
     CDS             complement(15338..15604)
                     /locus_tag="PLM79_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86627.1"
                     /translation="MERTTFCGIIAFHFPKNVEILSVPKDAHLANASGRHNSTYRREG
                     DAVTVHRSFRDQTPGPVRTPADDREFRIHPRHILRDLRAQVLYQ"
     gene            complement(15851..16096)
                     /locus_tag="PLM79_09715"
     CDS             complement(15851..16096)
                     /locus_tag="PLM79_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86628.1"
                     /translation="MQTLSSNGSKRPVPDPGQGIFALDSRFVREEAEIFVQGVRAVIA
                     FVQPNMGDLNVRSILESNGHTGTSTRLKADLRQMSDL"
     gene            16249..18339
                     /locus_tag="PLM79_09720"
     CDS             16249..18339
                     /locus_tag="PLM79_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86629.1"
                     /translation="MSERPTRRELEKRLAELEKEVRLRREVEKTLEDELEKFRGLYDL
                     AIAMTTDRTLDQNLQLIVDQCRRLLHSDISYLSLYDESRGDFYKHTCSGIHLEAFRKI
                     RLPPGKGLGGLVTRTQQGHIVEDYFGEAGLERLPDESIAEEGIVSGMAAPVRMGSRNL
                     GVLYVFNRSRIRYTAADLDTLSLIGNLAAVEVARKQVESSLRESEERFRIMAQTTGDV
                     IYRLHYDSMEYDYLSPGIRKLTGYSAEEIREIGFSRLVVRIDLPERENVSPSVIARNR
                     AEGKTGEFRADYLIRSKEGEHKWLRDHSFPWHDDLGRVAGSVGILSDISDYRRAEALV
                     RERTAELIKSEEKYRTLVENVPLVVYRMGLDGEVLFVNPFVEELFGYSAVEILANPRL
                     WKETVYREDLERVLEQRRKCCSRGEELMIEYRVRHKDGRLVHVLDHAIPSFVSEGVVG
                     SVDGIIIDVTGRRILQEKLIQAEGLRTIEEVSARLAHEIRNPLVSAGGFARRLLSSMS
                     PEDPNRQKVQIIVKEVGRLEVILRMILTYIQPLDLDVSPIRLNALVEKVLNALEPVVT
                     DKKIRLDLHLAPGLPKVPADPTLMERAVKTLIGNALSALSEEDVLRVSTGREENFIRM
                     EMRYPAQNLSSDDVEHFFYPFTTFQQSYSDIDLPMAKIIVNKHGGTIEARLDPPGGIA
                     LSLSLPLEPHRPSS"
     gene            complement(18650..18850)
                     /locus_tag="PLM79_09725"
     CDS             complement(18650..18850)
                     /locus_tag="PLM79_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOV86630.1"
                     /translation="MTEGRVKWFNDSKGYGFIESDSGSDVFVHFSAIEGAGFKSLSEG
                     ERVSFVEEAGRKGPQATKVRKL"
BASE COUNT         3723 a         5694 c         6049 g         3640 t
ORIGIN      
        1 cgatcgggag acgtggttct cctgtccccc gcatgctcct cctttgacca gtacgaaagc
       61 tattcccaga gggggaacca cttccagcaa ctggtttctc acctgggcaa cgaggcgaca
      121 ggctcatgaa ccccccagga acggcacttt tttcgagcca cggctcccca gggcagggaa
      181 aacgatgttg agacccagcg cagacacttc ggctttcggc atggaacccg ccgcccggga
      241 aaccgaaagc atcccggcat ccctgcccct cgagcttcat ctatggatcg tggtgtcgtt
      301 tctcgtcctt ttgggtttga tcatggtcta cagcgcaagt tccacggctc cgttcaagga
      361 gctggcggag agggcggcgt tgttgaagtc cccatcccat tacctgaaaa ggcaactcat
      421 atgtatcgct ctaggcatcg gaatcatcgt actggtacgg cgtattcccc atgggtggta
      481 caaggaacag gcgaggtgga tgtttctctt gtccatcgca gctttgatgc tcgtactcat
      541 tcctggggtc ggtgcagaat tcaaccatgc aagacgctgg ttccagtgga gagggttttc
      601 cctgcaaccg gccgagtacg ccaaggtgat ttgggtcctt tttctgagtg tctcgttggt
      661 taagaagcag gatcggatcc ggcaggtatg gatcgggttt ttgccccatg tgctgttgtg
      721 cggaattctc agcgtgctgg tgctcctcga gcccgacttt ggaaacacct tcgtcgtcgg
      781 atgcatcacg gtcctgttgc tggccgtcgg cggggtgccc ttcgggcagc tgttgaccct
      841 ggttcccgtc gccgtcctgg gattctacaa gttcgtctac ctggttccct accggtggga
      901 gcgcgtcacg gcctttcgaa atccctggac ggacccgttg gactcgggct accagctgat
      961 tcaggcctgg attgccgtgg ggtccggcgg cctgctgggc aaggggttgg gagccggtca
     1021 acagaagctg ttctacctgc ccgaatccca cacggatttc atcctggccg tcatcggaga
     1081 ggagcttgga tttgcgggca tcctggccgt ctgtgcgctt ttcttttttc ttttccgggc
     1141 cgggatgcag atttcgcgca gagcgcccga tctgctggga tctctgatcg ccttcgggct
     1201 gaccatgctc ctgtccatcc agggcctgtt caacatgggg gtcgtcctgg gattggttcc
     1261 caccaagggg ttgcccctcc cgtttgtttc ctatggaggg agcgcctttc tggccaattg
     1321 cgcggccgtg ggggtcctca tgagcatcgc ccgaagcagc gagaaccgaa gggaaccatc
     1381 ctatgtttaa gcgctaccag catattcatt tcgtgggaat cggcgggatc ggcatgagcg
     1441 ggattgccga gttgcttttg aatctcggct atcgggtgag cggctccgac ctgaaggaaa
     1501 ccgatctgac ccggcggctg ggggcactgg gggcgcgaat cttcgtggga catgcggcgg
     1561 aacacgtgcg gggggccgac gtggtggtgc tgtcttcggc catctccgag gacaaccccg
     1621 aatggctggc ggcccgcgcc ttgggcaagg ttcccatcat tcgtcgagcc gaaatgctcg
     1681 cggagctcat gcgcctcaag tactcggtgc tcatcgcagg ggctcacggc aagaccacca
     1741 ccacatccat ggtttccacg gtcctggccc ggggcggcct ggatcccacg gtggtgatcg
     1801 gcggcaaact gaacgcctgg ggcaccaatg ccaagctggg tcacggggac ttcatggtgg
     1861 ccgaagcgga cgagagcgac ggcactttcc tcatgcttcc cccgaccatt gccgtggtca
     1921 ccaacattga cctcgagcac ctggattttt accgggacct cgagcacatc caggaaacct
     1981 tcctccagtt catcaacaag atccccttct acggccaggc ggttctctgt ctcgaggacg
     2041 aaaacatcca ggccctgatg ccgcgcatcg gcaaacggtt tgtgacctac gggttttcct
     2101 cccaggcgga cttccaggcc cgggacgtga ggatcgacgg attcagggcg tcttaccggg
     2161 cctattgtcg cggtgaggaa ctgggagaga tcgagctggg gatccccggg cggcacaacg
     2221 tgctgaattc cctggcagcg gtggcggtgg cccgggagct cgagatcgcc tggccggacg
     2281 tgcagacggg cctgggggac atgaccgggg tccaacgccg tttccaggtc aagggggaag
     2341 tggacggagt gctcgtgctg gacgactacg ggcatcatcc cacggagata cggacggttt
     2401 tggaaacctt ggcccagtgt tttcccaacc gccgccgcat cgtggcgttt cagccccatc
     2461 gctacagccg cacccacgct ttgagggagc agttcgcccg gtgtttctac cattcggacg
     2521 tgctcctcac cacggaaatc tacgcggcga gcgaaaaacc catcgaggga gtgacggggg
     2581 aggggctggc ccgggagatt gcctcccacg ggcaccatga cctgcactat tgtcggaccc
     2641 tggaagaaat gctggagaag ctctgcgaga tggtcgttcc cggggacgtg gtcatgaccc
     2701 tgggggcggg aaacattctc caggtgggcg aatggctgtt ggataggctc gagcagagac
     2761 ggagggcatg aggtcgggat gggaagcgcg gcatcccaca ttcttccgcc ttcagacccg
     2821 gggaatcccg agggggactc ggtgcgggaa tttaaggcgc tttttcgttc cctggacgtg
     2881 gaggcggagt ggctcgagcc catgtcccgg cacacgacct ttcgcatcgg agggcccgtc
     2941 gcctgtctcg tccgccccct cgatgaggct tccctggcga aagccctggg ggaattgaag
     3001 cggctcggtc ttccccattt catcctggga gcgggatcca acatccttcc ccccgacgat
     3061 ccgtgggacg tggcggccgt tcaactcacc cgaagctgtt cggacatcgt cccgatcgag
     3121 gagcacgggg accgagttcg ggtccgggtc ggggcggggg ggatgctgtc ccgcctgctg
     3181 cgattttgcg ttcggcatca gttgggaggc atcgagttcc tggcgggcat tcccgggacc
     3241 gtgggcgggg cggtggtcat gaacgccgga acaaaacgag ggtgcatcgg ggaggccctc
     3301 ctgtgggtgg acgtgctgga tggaaacgga agacgccaca ggctggagaa atcccgcctc
     3361 tccgtttcct atcgttccct gggactgcct ccggacgccg tggtcctggg ggcgtgcctc
     3421 gaccttcggc ggagcgtcgg cgcaaaactg cggggcgaac tgctggaaat cctccggcat
     3481 cgccgggcca cccagcccct gggataccct tcggccggtt gtatcttcaa gaaccccccg
     3541 ggggcatcgg cgggggccct catcgagcag gtgggactga agggatggaa ggtgggggga
     3601 gcccagatca gcgagaagca cgccaactgg attgtcaacc tgggaaaggc ccgggctgaa
     3661 gaggtcctgg ccctggtgga agaggctgaa aagcgggttt ttgaagcctt tggactccgg
     3721 atggaacggg aaatacggat cctgggatga tcaaaaagaa gaaaaaccga tatcgctcgg
     3781 attctcgaaa ggcccggaag gcggtggcga ccttcctcaa ggccatcgga gcagtggttt
     3841 tgtccctggc gggaaccctg cttttgagcg ccgggctggt ccggggttac catgccgccc
     3901 tcgatgcccc cctatggagg gtcgaggaaa tccggatcac ggggaatcgg catctcgaac
     3961 gcagggacat cctgaacgtc ctgggagtgg aaaagggagc ctgcattctc aacgtcaaga
     4021 catcccagct tcgtcggcgc ctgtgtgcca tgccctgggt gcgccatgcc gaggtccagc
     4081 tcgattttcc cggctccatc ctcgttcgta tcgaggaaag gacccccctg gccattgtgc
     4141 acaccgagga cctcttcctc atggatgagg agggaaggct gttcgtgcgg gatcgtctcg
     4201 agaaccatcc gggcctgctg tacgtcacgg gattttcagc cctgggactg aaagaggggg
     4261 attttcttcc ccgggaaccc ctacagcaac tgaagggact cctgggcgcc ctggcgcaga
     4321 tccgggggag tctgccgccc cagttgatct ccgagtgtca ttggcggggg gaagagggtt
     4381 tcaccatcta taccgctccc agcgccgtgc ccgttcaatt ggggtcgcag gagtttgacc
     4441 tgaagatcga gaggctgcag atggtcctca gagtgctggc ggaacgtgaa tggttgaacc
     4501 tggtgacccg catagacctg aattatccgg atcgggcgta tgtggagggg cgttttccta
     4561 ccccaaaagg cacctgactt gcggggaggt gcccaatcaa aggcgggggc tccgggccgt
     4621 gtcctcgggt tcttgcgagg cagcgaccgg aagggccgaa aacacagggt gggcaggtag
     4681 ctgggcgccg tctttcctga gaatgacgtc gaaagggaac caatggggaa gagagaggaa
     4741 ctgatcgtag gccttgacct gggaaccacc aagatttgcg ccgtggtggg ggagttgacg
     4801 tccgaaggga tcgatatcgt aggggtgggc agctacccgt ccgtggggct acgggccggg
     4861 gtggtggtca atatcgatca gacggtcaac gccattcgca aggccgtgga agaagcggaa
     4921 ctgatggcgg gctgtgaaat ctcttcggtg tacgccgggg tcgccgggac tcacgtccag
     4981 agtctgaaca gccacggggt gatcgccatc aagagtcggg aagtgaacca gacggacatc
     5041 gaccgggtcc tggatgcggc caagaccgtc gctttgccct tcgaccggca gatcctccat
     5101 gtcctgcctc agcagtacat cgtggacgac caggagggaa ttcacaatcc gctgggaatg
     5161 gccggggtac gcctggaagc caaggtccac atcatcacgg gggtcaccag cgcggtgcaa
     5221 aacatcgtca agtgctgcga gcgcgccggg ctccaggtcc aggacgtggt gctcgaatcc
     5281 ctggctgccg ccgaatccgt gctggatccg gacgaaaagc acctgggagt cgccctcgtg
     5341 gacatgggtg gcgggacgag cgatgtggcc attttcagcg accacgccat ccggtactct
     5401 tgcgtggtgg ggctcggggg aaatcacatc acctccgaca tctccgtggg cctccgtacg
     5461 tccatggacg aggcggagaa aatcaagaag aagttcggtt gcgccctttc cgagcacgta
     5521 ggtccccaag atgtcatcga ggtggggtcc gtgggagggc aaaagccccg gcaactggcc
     5581 aagtccatcc tgacccagat catcgaggcc agggtggaag aaatcatgaa gatcatcgag
     5641 tgggagctgg tccggtcggg atatgtcgag tccctccacg ccggggtggt cctcaccggg
     5701 ggagtttctc tcctgccggg aatccgggaa ctggccgaaa gagcgctgga catgcccgta
     5761 cgcatcgggg ttccctaccg gttcggaggg ctgggggatg tggtcaagaa ccccatctac
     5821 tccaccgcca gcggtttgct tctctatgga aggaaacacg gaagccgtgg gggtcgtttg
     5881 gaagagccca gcctgttcaa aaaggtcatg gaatccgtga gacgatggtt tcgagagttc
     5941 tggtaatcgg ggccccggcg atggaaatct cccgaggaag gggtctcctc cgtcgctgcg
     6001 gcagcaaagg gcaggatttg gttggcaggc gtcctaacca aatactttca tcgaggggga
     6061 agggtgcgat atggagaatt cagcattgaa tcgggctaag atcaaagttc tgggaatcgg
     6121 cggcggcggc ggcaacgcaa tcaacaacat gatcagcgaa gggctggaag gagtggaatt
     6181 cgtcgcggcc aatacggatt tccaggtgtt ggagcggagt cttgcgcccg ccaagatcca
     6241 gctgggagtg aacctgacca agggactggg agcgggagga aacccggagg tcggcggcaa
     6301 agccacccag gaagacgtgg acaaaattcg cgccgccgtg gacggcagcg acatggtgtt
     6361 cattaccgcg ggactggggg gtggaacggg aacgggaggg gcgcccatcg ccgcccaggt
     6421 ctgcaaagaa atggggatcc tcaccgttgc cgtcgtcacc aagccctttg ccttcgaagg
     6481 aaaggtccgt caccgaaacg ccgacgaagg cctgaaatcc ctccaggggg tcgtggatac
     6541 cctcatcacc attcccaatg atcggctcct gtgcctggcc gaccgcaggg ccacctttct
     6601 cgagatgttc aaacgggcgg acgacgtgct gttgtacgcc gtaaagggca tctccgacct
     6661 catcgtgaac cccggctaca tcaacgtgga ttttgccgac gtgaagacgg tcatggggga
     6721 aatggggctg gccttgatgg gaaccggctc gggtcgaggc gagaaccggg cggcccaggc
     6781 cgcccagcag gccatctcga gcccgcttct cgaagacatc tccatccacg gagcccgggc
     6841 cgccctgatc aacatttgtg ccgggcccga cctgggcatg cacgagttcg aagaggcctc
     6901 ctccatcatc cagaaagagg tggacgacga agcccacatc atcctgggaa tggccatcga
     6961 tcccgccatg gaagacgaga ttcgagtcac ggtcatcgcc acgggaatcg ggaaggtgga
     7021 gaagtcggaa ccggtgagca ccgccgccgc ccgggccaaa agagccaagg tggaagggat
     7081 catccccccg gagctcaact acgactactt gcagatcccg acggtcgtcc gtcaccgtcc
     7141 ggttcgagac aacgggcccg gtgccggttc cgagcctccc aagaggagga cgtttcttca
     7201 aagactgacg ggggccgtgt ccgaggagga agacctcaac attccgacct tcatccgccg
     7261 tcaggcggat tgagaagggg gaagccgtcc ctaaacaggc cttccgtcgc tttcggtgca
     7321 atcgtctctt ggagacctcc tcctccgggg gggggctggg gtgaacccct tcaccgatcc
     7381 cacccccccg ttcctcaggt gggggtcggg gacaccgacc caacctcctt tccctcccct
     7441 gaaacggact gggacggggg cgcaggaagg ccgctttttg gggatggaac cccttgatgt
     7501 ggagtctccg gtccgaagcc ccggtttctt gcccgccggg tcctcggatt ttggcggggc
     7561 cttgcccccc ccttctccct ccgttgcgga gacgaaggcc gaggtgagtg ggcatcaaag
     7621 acgccgattt gtaccccact caccttgacc tcggccccgt ggaacgacac ggacccgacg
     7681 gggccgcgga gacgccgacg gcactcgagt cggggccgaa accacccaat gtccccgcgg
     7741 ggcctttcgg gccttccttg aagatccgcc atgcattgga aatggaagca gcgtcatcgg
     7801 cagcggcttt ccgccgaaag agggaccatc cgcaaggatt ggcgggggaa agtgcgcatt
     7861 gccctggcct tccccaaccg ttatgccgtc gccatgtcca acctgggttt ccagtcggtc
     7921 tacgcagcct tgaacgaggt ggaccacgtc gtctgtgaac gggtcttcta cccggaaacc
     7981 gccgatctgc cccatgtgaa acaacatccc ggaagccttt tgtccctgga gtcccagcgg
     8041 ccggtccggg agtttgacct ggtggctttt tccgtcccct acgaaaacga ctactcccat
     8101 gtcgtcgaaa tgctgagcct ggcgggcatc gaacctcggg cagcccgccg ttcccgggaa
     8161 tccccggtcg ttgccgccgg gggggtcgcg gtcatcctga acccggaacc gctttccccc
     8221 ttcatggatt tcttcttcat cggggaagcc gagggaatgc tgcccgacct cgagggccgg
     8281 tggggggaat tccccgagag aagggacgac cggccggact tcctgcatca gctggcccgg
     8341 gaagtctcgg gcatctatgt gccgtccctc tacaccgact cgtatcttgc ggacgggacg
     8401 ctccggtccc tcgatcccgt ccacaaggat ctgccggaaa aggttcccta tgggcggggg
     8461 aacctggatc gggccaagcc ctgtcggacg gtggtcctca cccccgagac cgagttcagc
     8521 gacgtgaccc tgttggaaat tggaagaggg tgcggccggg gatgccggtt ttgcgcggcg
     8581 ggctacgtct accgtcccgt tcgattccac gcgggaagcc gggtggtgga tgcggccgcc
     8641 gagcgtctcg gggaaagtcc ccgggtgggg ctggtgagcg ccgccgtctc ggatcatccg
     8701 gaaatcgatt tcatctgccg gtccttgttg gaccggggtg cttccttgtc cttttcgtct
     8761 cttcgcgccg acggcatcac cccggagatc gcctcgaccc tcgaggcagg ccgacaccaa
     8821 gcggcggccc tcgcccccga ggccgggtcg gatcgcctgc gaaaactcat caacaagaat
     8881 cttaccgtcg agcaggtcca ccgggccgtg aaggaactgg tagacaaagg ggtgatgcac
     8941 ttgaagctct acttcatgat cggtcttccc acggaaaccg ccgaggacct cgaggccgtt
     9001 gtggacctgg tgaagggaat ccgccacgag atcttgaagt ccagccgggg tcgcactaga
     9061 ttgggaaccg ttaccctgag cgttcattcc tttgttccca aaccgttcac tccctttcag
     9121 tgggcgcctt ttgccggggt ccgggaactg aaggaccggg gaaagaggct ccaaagaacc
     9181 ctgcaaaagg tcgccaacgt tcgcgttcat ttcgatcttc ccaagtgggc ctatgtgcag
     9241 gccctattgt cccggggaga tcggcgggtg gcttccatcc tcgagaaagt ggccctcgag
     9301 ggagtcggct ggacccaggc catgaggagc gccccggcga acccggattt ttgggtcatg
     9361 agggagaggg gaaaagagga aatctttccc tgggaaattg tggatttggg attgaaaaag
     9421 agcttcctgt gggaagaata ccggcgggcc cttcgcgggg agcccagccc cccctgccgc
     9481 ccgcacgaaa actgccgccg ctgcggagtc tgtgcgccgt agaaccccat tgccaaaaaa
     9541 cactccggtt aaggcctcat gggcggttcg ccctcccccc cgggcaaagg ggagaaaacg
     9601 gggggcctcc cgccaaggga ggagccaaag ccttcccccc ggttggcaag agacaatccc
     9661 gtttgttaga agagtaccct gcacaagggg ccatgtcgcc cgcggttgga ccgaagcgcg
     9721 ttcctcaatc ccgcccgccc aggcggggac ccgggattct tcagggattc cacgcctcct
     9781 ctttccccgc ccgattcctg cggagacaga ccttgcgggg gttgcgtcga gtcgaaaatc
     9841 tcgtccgttc cggtcacgga cggatcgccc cccatagacc gggtcctcga gggttgcacg
     9901 gaggcgcttg ggtctgcagc cgctcccggg acaatccgtt ctccgcagag gatctccggg
     9961 cccagacttt ccactttctc gcccctggga aaatcctacc tgtttgccga gaaagtccca
    10021 taatcggcaa gggagtcgtt cacgaaaacg tggtaggtgc cggtaatctt ggtgccgccg
    10081 ctcgagagct ttcctttggt gaatttcagg accatcttgt cttcgcggca gtcgaagacg
    10141 gctttgaact tggccgtggt gtttgaaacc gatccgttga gattggtgga gacggtaccg
    10201 caatgggtat tggtgactcg caaagtgccc ttgagcacgg tgccgctttg cttgacgacc
    10261 gccttaatcc tgccggaacc cccaaaatag ctgaaccact tgccggtcca ggtaccctca
    10321 acccgcggga cggataccca gatggcggcg ggatcttccg tctcttgggc aactccgacg
    10381 gccgcggtga gcaaaagaac catgagggcc actcccaagg aaagcttctt catcctgttc
    10441 tcctttctcg tgcaaggcag gctcctcccc gccaatttcc aatttatccc actcccggtg
    10501 ccgctgttca tcttccgtac cgccgcgttt cggcaaacgg gttggcaccg tccgttgccg
    10561 tccgactaag gaattggaga taaacctgaa accccgtctt gcggcagggc ggcttcatcc
    10621 ctaacccacc aacacaaaag gagaatcagg ccgcacggca tggtaacaca aatctttccc
    10681 gggtgtgaaa ccggaaaatg ggaggcagga gcaaagagaa cccgggcgta caaacgcttg
    10741 cctcgaaaac tcagggcgga gcaagcccct ttccatggcc gtacgatcct tcgataagat
    10801 ttgacgctct cggcctacac gggtacattt ccgccgtgtg caggcggatc atcctcaacc
    10861 gaatcccgga tgccttgccg gatatcccca ttgttttcct gtgcggcgca aggccaccag
    10921 gaaagagcac ctttgtccgg gaaatcgggc tgggtcgtgt ccggcccgtt gtgcgaatcg
    10981 ggatgacgcc gggggccttc ccccttccgc tcacgggcgg ggccgcaagt ggatgtcgct
    11041 gcgcaggaca gtgggggaag gtcaatgtcg gaaccacctc ggggagaaga taaagccgga
    11101 tgattgttct ccattccgcc atcggcgaag cgggtctcta cctatggggg gagagcccgt
    11161 ccgaagggca ggccgggccg cgaaggaggc ccggaagaaa gcccaaagca ccatccacca
    11221 agccgctccc ttacgacgcg ggaccggaag ccctcggaga ggcgctttcc gaagggggaa
    11281 tggctgcggg gaaaacgggg attgaaccgc tcaaggggtt tgtgtggata ccctcggcgg
    11341 gaagcattcc ggcggcatcg agccccctca tagccgaacc gcccggcgtg gaggggaagg
    11401 tgcaggtttc tccgtgggtt gtctccattc tcgccctgga tggttccagg agcttcgaat
    11461 tcctctgccg ttgtgtgggc aagacgatcc tttcccccgg aaccatcctg ggagacgacc
    11521 tcctttttct cacaaaagcc atgcagtttg ccgccgcgct cgtggcgaag cagcagttct
    11581 tgcccggcgt ggcacgggtc gggagaagct attttgccag gtggaagccc gtgtttttgg
    11641 gtgccgacgg agaacggctc ggcaggctcg ccggggccat gccgtccgcc tgcagggccg
    11701 tccagttcca ggccgactcg ccgcccgaga ccccttccct caccctcctt tccaacttcg
    11761 tgggaggcat ggtcgatcac ctggttcgct tctccgccgc tccgggtccg ttcgagccgc
    11821 tgccgtccag gggaaggaag aaggccgccg ttttcgacag tatccacgac cagtggcttc
    11881 atgcgttagg caccccggac ggaaagatgg acgggagcga agccgagctt gcccgattcg
    11941 ctctccaggt cgaggactgg caccggccgg tgacaacctc ggctgcaagc gccttcaggc
    12001 tttgcttccg tctggatgag cccgggccgg cagtcgaggc agcctcgacc ggaagacgag
    12061 gtggggcgag tccgcaggac ggtgaaggct ggacggttca ctaccttctc caggccgccg
    12121 acgatcccag ccttctcgtg ccggccgagc acgcctgggc ggccaggggc cgcagggcgt
    12181 cggtgctcaa gcgggagaat ttcaatccca gggagtatct tctctgcgcc ctggggcagg
    12241 cctccggcct atgcccaaac atcgaggcga gcctcaaggg ccccgtcccc cggggttacc
    12301 gtctcgatac cagcggggcg tacgactttt tgacccaaag agcttgggcg ctcgagcagg
    12361 ccgggttcgg agtcctgctt cccgcctggt ggacgcgcaa gggcaccaag ctcaagctca
    12421 ccgcccgtgc cggggtgaaa agtccccgga tgcaggccaa ggcaggcctt ggcctcgatg
    12481 agatcgttca gttcaactgg gagctcgccc tgggcggcga accgctcacc ttcaaggaac
    12541 tccaggaact cgcccggctc aaatcacccc ttgtgagggt acggggccaa tgggtcgagc
    12601 tcaaccccga agagatccgg gccgccgttg atttttggaa aaagcaggcg ggcggggaaa
    12661 ccacggcccg tgaggtggtg aagatggccc tgggcgtcca cggggccccc ggctcgattc
    12721 ctttcggagg agtccgggcc acgggctgga ttgcggatgt cctctcccag ctcgagggac
    12781 gcacgccctt cgaggaactc ccggtgcccc gacggtttcg agggaccctc cgtccctatc
    12841 aggttcgagg gtactcgtgg cttggctttc ttcggcgatt tgggctcggg gcatgtcttg
    12901 cggacgacat ggggctgggc aaaaccgtcc aggccctttc cctgattcaa aaggactggg
    12961 aatcgaacgg cgggaagccc gtgctcctca tcagtcccat gtccgttgta aacaactggc
    13021 gcaaggaggc ccggcggttc actccggacc ttcccgtcct ggtccatcac ggggccgcaa
    13081 gaagcaaggg aaagactttc aaatcccaag ccgggaagca tgccctggtc ctgtcgagct
    13141 actctctcct gtaccgcgac ttcgagaccc taaagagcgt tccatgggcc ggcatcgtcc
    13201 tggatgaggc ccaaaacata aagaacccgg aggccaagca ggcgaaggcc gttcgggccc
    13261 tcgaggcggg ctaccgcatc gccctcaccg gcactccggt tgaaaacaac gtgggcgatc
    13321 tgtggtccat catggagttc ttgaacccgg gattcctggg gtcccagagc gaattcaggc
    13381 gcaagttctt cattcccatc caggccaacc gggacccgga ggcgacggag cggctcaagc
    13441 gcattaccgg cccattcgtg ctaagaaggc taaagaccga caaggccgtc atcgccgacc
    13501 ttccggacaa gttggaaatg aaggtctatt gcaccctcac caaggagcag gcgtccctgt
    13561 acgaagccgt gctcaaggac ctcggggaag cactcgaggc atccgagggc atccagcgca
    13621 aggggatcgt gcttgcgacc ctttcaaagt taaagcaggt ctgcaatcat ccggcccagt
    13681 tccttgggga caactccgcg attccgggtc gatccggaaa gctggcccgc ctgaccgaga
    13741 tgatcgagga aatcctccat gtcggagacc ggaccctcgt tttcacccag tttaccgaga
    13801 tgggggccat ccttcggcgc cacctccagg agaccttcgg ccgggaggtg ctcttcctgc
    13861 acggcgggat cccgaagaaa cagcgggatc acatggtcga gcgattccag ggagtcgaga
    13921 acggtcccgg gatttttatc ctttccctca aagcgggagg aaccggattg aatctcacga
    13981 gggccgccca tgtcttccat ttcgaccgat ggtggaaccc ggccgtggaa aaccaggcga
    14041 ccgaccgcgc cttccgcatc gggcagacga aaaacgtcca ggtccacaag ttcatctgcg
    14101 ccgggaccct cgaggagcgg atcgacgaga tgatcgaaaa gaagaaggaa atctccgaag
    14161 gcgtggtggg atcgggtgag gcatggctta ccgagctctc cacaacggag ctcaaaaagc
    14221 tcttcgccct tggcaaggac gccgcggcgg aatgagacgc cctgaatcgg taggacggtg
    14281 aaccggggaa aggacgacag gcaccggaac aggggtacgc gcggagcgga agaaaggaga
    14341 ccggcttatg tcgtggcggt attatgatca tttcccgaga tcgaccccca aggccgccaa
    14401 aggcggcatc aaggcacaat cgaaacgagg cggattcggg aatagttggt gggcgaaacg
    14461 gtggatcgcc gtcctcgaga gcttcaacat cggagagagg cttggccggg gacgatccta
    14521 cgcgagaaga ggccaggtgc tctccatcga catcgaaaaa gggaaagtga aggccagggt
    14581 acagggctcg aggcccagac cctatgaggt cgccctccag gtgaaggtcc tttccccggc
    14641 cgattggagg aagctcgccc aggcgctctc gagccggata ctcttcgttg caaagctcct
    14701 cgcgggcgag atgccccagg acatagaaca ggtgtttgat ggggccggac tgtcgctttt
    14761 cccgagcaag tcgaaggacc ttgccaccag ctgctcctgc ccggactggt ccaacccctg
    14821 taagcacatt gcggccgtct actatcttct cggggaggaa ttcgatagag atcccttcct
    14881 tatcttcaag cttcgcggga tggaaaggga ggagctgttg gccggcctgg cacagactgg
    14941 gaaaggcggg agcaggaagg agttcggagg catggagtgt cccggcgcaa cggaggacgc
    15001 agccccccct ctcgatccga tctgcgccga tggggaggta ttttggaaag ggagcaggct
    15061 cccggaggat ttgctgggag aagtccgcat ccctcccttg cgggcggctc tcctgaaacg
    15121 gctcggcggt tttcctttct ggcgcggcaa ccggctcatt caggaagccc tggaacccgt
    15181 ctactccaat gcctcccaac gtgggatgga tatcttcctc tatgaatcgg aacgcggccc
    15241 ttcccgctag tcccgcaaat cctgtccccc tccgattcga caggccgacc gtccgcttct
    15301 tccaggtgac cggcccatga tttggccccg caaccggcta ttggtaaagg acctgggccc
    15361 tcaggtcccg taggatgtgc cgggggtgga tccggaattc cctgtcgtcg gcgggggtgc
    15421 ggacggggcc cggcgtttgg tcccggaagc tccgatggac ggtcacggca tcgccctccc
    15481 ggcgataggt ggaattgtgg cgccccgagg cattggccaa atgagcatcc tttggtacgg
    15541 acaggatctc gacgttcttg ggaaagtgaa aagcgatgat cccgcaaaaa gtcgttcttt
    15601 ccaaaacgga acctcatgaa ttcaatcggt taggatcgaa gacacgcact tttcaggcct
    15661 ttttgcgaga ccatcaaagg tgtattcctc ggtggacaag ccccccaagc aggcgcggcc
    15721 ggggtccttt ccggcaggct gaggccggag actaccaacg agatgggggg tccgccgggg
    15781 aacaccgggc caatgagaag ggccccggga ggttcacggc attggccggg tgaaacttcg
    15841 ctccgggggt ctagagatcg gacatctgcc ggagatcggc cttgagccgg gtgctcgttc
    15901 ctgtgtgccc gttgctttcg aggatgcttc gcacattgag gtctcccatg ttgggctgaa
    15961 cgaaggctat gacggcccgg accccctgga caaaaatctc cgcttcctcc cggacgaagc
    16021 gggaatcgag cgcaaagatt ccctgtcccg gatccgggac cggccttttg cttccattgg
    16081 aggatagggt ttgcatcgga agcaaggatt cgtgtagatg tgatggaaga agacttctcc
    16141 tccggttgca cgggattcgg aggagaggga ttccccgcgt tgctcggccg acggtcgggc
    16201 cgccgtcccc ggggcgcttt tggaaacgga tccacggagg atcttctcgt gtcggaaaga
    16261 cccacccgcc gggaactgga aaaaagactc gccgagctcg aaaaggaggt gcgtcttcgc
    16321 agggaggtgg agaaaaccct cgaggacgaa ctggaaaaat tccggggcct ctacgatctc
    16381 gccatcgcca tgaccacgga ccgaaccctg gatcaaaacc tccagctcat cgtggatcag
    16441 tgccgtcgcc tgctgcattc ggacatctcg tacctttctc tttatgacga aagccggggg
    16501 gatttctaca aacacacctg ctcggggatc cacctggagg cgttccgaaa gatccgcctt
    16561 cctccgggca aaggcctggg gggattggtg acccgaaccc agcaggggca tatcgtcgag
    16621 gattatttcg gagaggcggg gctggaacgt cttcccgacg aaagcatcgc cgaagagggc
    16681 atcgtctcag gcatggcggc tcccgtgcgg atggggtcca ggaacctggg cgtcctctac
    16741 gtcttcaatc ggagccgcat ccgctatact gcggcggatc tcgataccct gtcgctcatt
    16801 gggaacctgg ccgccgtcga ggtcgcccgg aaacaggtgg agagctccct gagggagagc
    16861 gaagagcggt ttcgcatcat ggcccagact acgggggacg tcatctaccg cctccactac
    16921 gattccatgg aatacgacta cctcagcccg gggatcagga aactgaccgg ctacagcgcc
    16981 gaggaaatcc gagagatcgg gttttcccgg ctggtggtga gaatcgacct gcccgaaagg
    17041 gagaacgttt ctccttcggt gatcgccaga aatcgtgccg aagggaagac cggggaattt
    17101 cgtgccgact acctgattcg gagcaaggaa ggagagcaca agtggctgcg ggaccattcc
    17161 tttccctggc acgacgactt gggcagggtg gcgggttccg tggggatcct cagcgacatc
    17221 agcgactacc ggcgggccga agccctggtg cgggagagaa cggccgaact catcaaatcc
    17281 gaggaaaaat accggaccct ggtggaaaat gttcccttgg tggtgtatcg catggggctg
    17341 gacggagaag tgctcttcgt gaaccccttt gtcgaagagc tgttcggcta cagtgcggtc
    17401 gagatcctcg ccaatccgcg gctctggaaa gaaacggtgt accgggagga cctggaaagg
    17461 gtgttggaac agaggcggaa gtgctgcagc cggggggagg agctcatgat cgagtaccgg
    17521 gttcgtcaca aggacggccg cctggtgcac gtgttggatc acgccatccc gtccttcgtt
    17581 tccgagggag tggtcggaag cgtggacggc atcatcatcg atgtgaccgg ccggaggatc
    17641 ctccaggaaa agctcattca agccgaaggg ctgcgcacca tcgaggaggt ctcggcccgc
    17701 cttgcccacg aaattcgaaa tcccctggtt tcggcggggg gattcgcgcg ccgcctcctt
    17761 tcctccatga gtcccgaaga ccccaaccgg cagaaggtac agatcatcgt aaaggaagtc
    17821 ggacgcctgg aggtgatcct tcgcatgatt ctcacctaca tccagcccct ggatctggat
    17881 gtctccccga tccggctaaa cgctctggtg gaaaaggtcc tgaacgccct ggaacccgtg
    17941 gtgacggaca aaaagatccg cctggacctt cacctggctc cggggctccc gaaagttccc
    18001 gccgacccga ccctcatgga acgggccgtg aaaaccctga tcggaaacgc cttgagcgcg
    18061 ctctcggaag aggatgtctt gcgggtttcc acggggaggg aagagaattt catccgcatg
    18121 gagatgcgat atccggcgca aaacctgtcc tcggatgatg tggaacactt cttctatccc
    18181 ttcacgacct tccagcagtc ctactccgac atcgatcttc ccatggccaa gatcattgtg
    18241 aacaagcacg gcggaaccat cgaggcccgg ctggatccgc cgggtggaat cgctttgtcc
    18301 ctgtccctcc ccctggaacc ccatcgcccc tcatcctaat ccgcgttcgg gaggtccttt
    18361 tccggcaccc ggcgccgaaa gcatcggcgg ggttcgtcga tggggaatcg tcctccccgg
    18421 gggcggcttc cggcggctcg atgccgggat ccgcattccc ccggcttgaa aaacgatccc
    18481 gagccctctc cggtcgcccg gtcccgaatc cctccggcct acctttagag tcatggcctt
    18541 ttcgggggac tcctccgggg gcggaggcag gtaccgaaga gcgtcgagta acaaaaaggc
    18601 cggagccaat tggccccggc ctccaatgct taacccggat acgatgagct tacagcttcc
    18661 gcaccttggt tgcctggggt ccctttctcc ccgcctcttc gacgaaacta accctttcgc
    18721 cttcgctcag agacttgaac cctgctcctt cgatcgcact gaagtgcaca aacacatcgc
    18781 taccggagtc gctttcgata aaaccatagc ccttgctgtc attgaaccac tttactcgac
    18841 cttccgtcat cttctaaatc cttctcttat tgggaatttt ttctgctgtg gggcgcggca
    18901 ctcggagctc ctgcgaaacc gcgctttgcc gtggcaaaag gtcgatttcc gtgtcactca
    18961 aagtccttcc tcaaacaagc atcgcggaat aataacggga gtcctcgaaa agacaagggc
    19021 aaaaggggaa tatttggcag ttgcagcgtt tttttcccgc cgcggcgttc ccgaggtccc
    19081 ggcccccctg cgggctaccg accgtc
//
LOCUS       DAOOVC010000064        19104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_1964_length_19104_cov_7.077694, whole genome shotgun sequence.
ACCESSION   DAOOVC010000064 DAOOVC010000000
VERSION     DAOOVC010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19104
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1964_length_19104_cov_7.077694"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /gene="nadA"
                     /locus_tag="PLM79_09730"
     CDS             complement(<1..273)
                     /gene="nadA"
                     /locus_tag="PLM79_09730"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOV86631.1"
                     /translation="MNESVIQRILQLKAERRAIILAHNYQPPSIQDIADLTGDSLELS
                     RQAASTDAQVIVFCGVAFMAETAAILNPHKKVLLPRPDAGCPMADMI"
     gene            complement(285..1043)
                     /locus_tag="PLM79_09735"
     CDS             complement(285..1043)
                     /locus_tag="PLM79_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOV86632.1"
                     /translation="MMDRSGKKGRTNALRRGAGLLLVFLLGVNLPSAEAAALSVKEIL
                     KKTESHYQQTQAFTARFRQWTTASAARSMASTEASGTLYYQKPRRMHWQYDKPEPQSF
                     VANNQLAWLYVPSEKTISFYDANTFFASPLVQTLFDGVIELKNHFEISLDSKQSSKQT
                     AVLKLVPRKEDPTLQSLYLWIELDSYRIQIIESHDALGNTNRVALESQRVVSSLDPKL
                     FQLDIPPSTVLLDTDGRELSPADVEMLKKKLTIQ"
     gene            complement(1040..3280)
                     /locus_tag="PLM79_09740"
     CDS             complement(1040..3280)
                     /locus_tag="PLM79_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOV86633.1"
                     /translation="MKHKKHEIWALCLAVLSLLALFSVISYHGADFVYFDAPSSSQRI
                     PPAVQNRVGSFGAHLAWLLITPFGLGAYALIVGGFVLSWCLFRGRLSLPQAYVPVGGA
                     VLVGISLITLLAIQAPDVRLFVFGGEKIRSGGSLGLFLSEVLLKSLYRLGAHIFLVGA
                     LLLGLLLSTPMSLASLWTWVGRICVRGGKVFSGAVRRIAALFSRQPEQTRPVSPVPRE
                     APRAKEVKRPAPPVEVLLPVPVTPKVRKPSRQVPPFVAPPKTSGLYRLPDLDLVDAYE
                     VENHKPDWKKLEDNARILEEKLADFGVQGKVMDIAPGPVITMYEYAPAPGIKISRIVG
                     LADDLSMALKAISIRVVAPIPGKAAIGIEIPNHSRETVSIRAVLESEAYLTSPSPLTI
                     ALGKDITGQPVVANLAKMPHLLIAGATGTGKSVCINTLLSSLLFRKTPEDLRLLLIDP
                     KRIELSTYEGIPHLIHPVVTDAKMATRGLRWAVEEMELRYKLLADKNARNIEGYNKII
                     SKEKPRAKGADPSAEDSGLPHHRLPYMVIIIDELADLMMVASREVEESITRLAQMARA
                     AGIHLILATQRPSVDVLTGIIKANIPTRISFQVSSRIDSRTILDTGGAESLLGSGDML
                     FLPPGTAKLQRIHGAFVSEQEVQRLTEFWRSQKLVEDPLRDRVRFEEASSSGDLSEEE
                     LDEKYEEAVQLVLDTRQASISMLQRRLRVGYNRAARMIEIMEQQGIVGVSDGIKPREV
                     IGRRDS"
     gene            complement(3311..4996)
                     /locus_tag="PLM79_09745"
     CDS             complement(3311..4996)
                     /locus_tag="PLM79_09745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOV86634.1"
                     /translation="MSYASDSESVLKVIPLGGLGEIGLNMMVLEYEDTLLLVDAGLMF
                     PEDDMLGIDIVIPDFAYLHRNRQRLRALIVTHGHEDHIGAIPFLLRDFQIPVYGTRLT
                     LALIREKLREYGLADRTKLIEVAPRQKISLGPFGVEFIQVCHSIPDGVGLAIRTPVGI
                     LVHSGDFKIDHTPVDGKRFDMARFGAYGEEGVLALFSDSTNAERPGYTLSEKDVGQTL
                     REVFQECAGRIIVAVFASNLHRIQQVIHLAREFNRKVLLNGKSMVGNVRIARELGYLD
                     FPPGEDITLQDLPRFADSQILMLTTGSQGEPMSALSRMAFNDHKKLKIKAGDTIILSS
                     RFIPGNERTIQNIINNLCRQGAEVIHERVRDIHVSGHAYREELKTMINAVRPRYFIPI
                     HGEYRHLVKHRHLAMIMGIPSQNCLLAENGDVISFQGNGKAAVGERVETGRVFVDGKG
                     VGDVAGVVLRDRRHLSEDGLVIASLVLNNETKEILSGPDILSRGFIHEGTKPEILDDA
                     KCVVLEVFDRMLLDPEFEVDCAELQAEIRKELKRFFGRVLDRHPVIYPIVVGI"
     gene            complement(5015..5791)
                     /locus_tag="PLM79_09750"
     CDS             complement(5015..5791)
                     /locus_tag="PLM79_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV86635.1"
                     /translation="MKKLLRSFLFFGSLLVSTIVLGSVAVGVSHLPRGSDRAHRCGRL
                     WARINLWAAGVRVRIRGLDRIDPDRSYIYAANHQGWFDIFALFARLPVQFRFLAKKEL
                     FRIPILGRAMSACGYIPIDRADRRKAFQSIDEAALRVRNGTSILIFPEGTRSPDGVMK
                     DFKAGGFVLAVKSQQPIVPVSISGSSRILPKGSTWLIQPGPIHISVGSPIPTEGSTNK
                     DKDILMSAVRDAILRGLTREEGGVMTKEDLARWDGGVRTA"
     gene            6166..6645
                     /locus_tag="PLM79_09755"
     CDS             6166..6645
                     /locus_tag="PLM79_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOV86636.1"
                     /translation="MARGVNKVILIGHLGADPDIRYTANGTPVANLRLATTDAVMVGE
                     GKWEERTEWHRVVAFGKVAENCGTFLSKGRQIYVEGRIQTRQWDDQQGNKRYSTEIVA
                     REIQFLGGGGGGEDRSAQAPRGGPSPSPAGTPSSYSNRSQTEELPPGPPGPDEDIPF"
     gene            complement(6872..7078)
                     /gene="tatA"
                     /locus_tag="PLM79_09760"
     CDS             complement(6872..7078)
                     /gene="tatA"
                     /locus_tag="PLM79_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOV86637.1"
                     /translation="MLPSIGMPELLVILVIVLIIFGAGKLPQIGEGLGKGIRNFKKAT
                     SDPLETSSEKVDKIEEKAPADKAS"
     gene            complement(7112..7513)
                     /locus_tag="PLM79_09765"
     CDS             complement(7112..7513)
                     /locus_tag="PLM79_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86638.1"
                     /translation="MAEIIEFGRKVQDLKSCRDQGLRQRKIDALKKIFQCTRCMLKCA
                     KCGTQLDMETEESSKFATPYPFCRNCLDEYEEYRARTGGKESGPRFYWHNDVWMKVWE
                     SWLEHQKHLDRYRQSKEFLQLLEEVEDLLKG"
     gene            complement(7527..8882)
                     /gene="hemA"
                     /locus_tag="PLM79_09770"
     CDS             complement(7527..8882)
                     /gene="hemA"
                     /locus_tag="PLM79_09770"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOV86639.1"
                     /translation="MSQLVLVGMNHKTAPIEIRERLAAVCREDKHLLHRFPQLRYVDE
                     LFVLSTCNRVEILFVCNQLHEATHEVKSLLRGHLGDALSAQVESCFYTYQFLDAVKHL
                     FRVASSLDSMVVGEPQILGQIKAAYREATECKAVGVILNRLLHKAFSVAKRVRSETMI
                     GSHAVSISYAAVELARKIFRDLRDKRVLLIGAGEMAELAAEHLLAQGVRQLMVANRTL
                     ERAVELAGRFKGETVSFSHILDALKNVDIVLTSTGSPEPILRHKDVKSRMRERKNRPL
                     FFIDIAVPRDVEQRVNEIENVYLYDIDDLQGIVDFNRAGRQLEAERAEHIVAEETVKF
                     QQWLCTLDVVPTIVGLREKADAIRRQELLRTLSHLPHLTEKDRKAIDVLTQSIVKKLL
                     HDPIMFLKKKAHRGSKQLFTDCTQQLFNLTNGCKPDSSALTDEVSSVKNENKILKNLE
                     K"
     gene            complement(8891..9712)
                     /gene="ccsB"
                     /locus_tag="PLM79_09775"
     CDS             complement(8891..9712)
                     /gene="ccsB"
                     /locus_tag="PLM79_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOV86640.1"
                     /translation="METVFLILATIAYCVGAIGYVIYLVRDQDAIHRVSSGILLAGAT
                     LQGLALIMRSISMGRLAVTNSQEALSFFAWVLVVVYLVVQTRFHLRILGSFVSPLAVL
                     FMLGSPLVPARMIPTSGIFQSVWVVVHVSALFLANALFALAFSAGIMYLLQERTIKRK
                     NFGFLYLRLPSLESLDKLNYVCLVFGFPLMTLGLITGFAYAGSVWKSPWNWDPKEIFA
                     LITWFIYAILLHERLAVGWRGRRAAWLAIFGFSAVLVTFLGVNLVLQGHHTVFVR"
     gene            complement(9694..10386)
                     /locus_tag="PLM79_09780"
     CDS             complement(9694..10386)
                     /locus_tag="PLM79_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HOV86641.1"
                     /translation="MSPSYYPMFVSLEGRVCLVVGGGTVAERKIRGLLPSGAAIRVVA
                     RTLTSGLWEWVRDGRVQYLGETYREDLLEEADLVFAATSDAGLNEQVARQAGERRLWC
                     NTATGPEMGSFIVPSLIERGPLAIAVSTTGLSPAVARQIREKLEGEFGPEWASFLEWM
                     GRLRKLILSKSLGSDEKQRLFRKVSNLPLPAWIREGRREEAFHAVLEVCNGWIHREEL
                     SRIWDELWKPSS"
     gene            complement(10820..11863)
                     /locus_tag="PLM79_09785"
     CDS             complement(10820..11863)
                     /locus_tag="PLM79_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOV86642.1"
                     /translation="MDQISTLPGESPVLPKLFQPEIEDIKERILVRIRPDLERIETEM
                     NRLLTTSVPLISVVGRYILRSGGKRLRPLLMILSARLCGYRGNRDVPLSVVFEFLHAA
                     SLLHDDVVDHAELRRSKPAANTIWGNPAVVLVGDFLYSKAILMTVGYDNMRILEVLSQ
                     ATTLMSEGEVLQMVRSDDLEVEEKEYLEIITRKTAVLISAACHIGAIFGGGGAEEESA
                     LRSFGLSLGLAFQLIDDALDYMGSPNELGKPVGNDLREGKATLPLICALMNGRSAEKN
                     RLREIFSGEEITQEAFDEIRNLVIRSGGIEYTQRLAMEYVQKAKAALHIFPSNPTKTL
                     LCDMADYVIYRRV"
     gene            complement(11962..13383)
                     /gene="rimO"
                     /locus_tag="PLM79_09790"
     CDS             complement(11962..13383)
                     /gene="rimO"
                     /locus_tag="PLM79_09790"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOV86643.1"
                     /translation="MKPLAALISLGCVKNLVDSETMVPQLVRSGYAMTEDPAEASIIV
                     VNTCGFLQSAVEEAVQSILEAAKFRSAGSCECLVATGCVVQRYGKKLAELLPEVDLFL
                     GTSHYHRLGDILAERQRGNGLRVWISTPRHLLGSDSPRIRATPPHTAYLKIAEGCNHS
                     CSFCIIPHLRGRYRSRGISDLVAEAARLADEGVKEINLIAQDTTAYGTDQGGTARLVP
                     LLDALEKVQGIEWTRLLYAYPDGVTDDLLDWMAQSRKTVPYLDVPIQHCSAGVLERMG
                     RRTAEDPLSLIRRIRSKVPHISLRTSLMVGFPGETEEEFRELFEFLEVAQFDHAGVFA
                     YSPEPGTAAARYSHPLESRIKEKRKRALLQRQRRISRRRLRRWVGKVLPVLVEGYHPE
                     TELLLGARLPTQAPEQDGMVLITRGTASPGELAMARITAAHDYDLEAELADPVIRPPG
                     SAGSGLPGRELRPGKGTRKRFDR"
     gene            complement(13437..14390)
                     /locus_tag="PLM79_09795"
     CDS             complement(13437..14390)
                     /locus_tag="PLM79_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86644.1"
                     /translation="MRWFPERLLPTAGMAAGLVVFLWAGLQMSRKVEPYWTWYYCFAW
                     WSYILFFQSLLHRRGGDSMLFRRPSRFFLLSLFSINLWLVFETWNFRLNNWEYHNVPP
                     GTFTRWVGYAVSFATVIPGIFTTQSLLDHLRVLEPGRPAKGGPSPRFHQVFLGVGGLF
                     LLLPLIWPRHFFPLVWGGFVFLLEPINYRLGAPSLLRQWEEGSLRTFYLLLAAGLVCG
                     FLWEFWNFWAGTKWQYTIPHVGRLKIFEMPILGFLGFPPFAVECYVMTSSFLALCRRI
                     GEEGLGQRRFRLRFLLGAGMVAWDLLIFHGIDLFTVASFSR"
     gene            14619..14837
                     /locus_tag="PLM79_09800"
     CDS             14619..14837
                     /locus_tag="PLM79_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86645.1"
                     /translation="MAKERVCRGHAAQSERNGKTLGPAMCKEGKGVRNFACQNYDLCL
                     DRAAKAMWSGFTCRDCAHYYQKEEEATP"
     gene            complement(14985..15443)
                     /gene="rplQ"
                     /locus_tag="PLM79_09805"
     CDS             complement(14985..15443)
                     /gene="rplQ"
                     /locus_tag="PLM79_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOV86646.1"
                     /translation="MRHGVSGRKLNRTTSHRSAMFRNMVTSLLQHERIYTTVQKAKEL
                     RRWADWMITLGKRGDLHARRQALATIPLKSVVHKLFAELAPRYQNRPGGYTRIVKVGY
                     RRGDAAPMCIVELMTEPKEGRKKAKAKAAQPKVSPAETRPAAPAPPVEGQ"
     gene            complement(15446..16453)
                     /locus_tag="PLM79_09810"
     CDS             complement(15446..16453)
                     /locus_tag="PLM79_09810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOV86647.1"
                     /translation="MQRNWRELIKPKRLEVETKDRPNNYAKFVCEPLERGYGVTLGNA
                     LRRVLLSSLQGSAITSVKIEGVLHEFSTIPGVLEDVTDVILNLKEVRLKMHGDGTRLV
                     VIDKQGEGAVKAGDIRGDAHVEVLNPDQHICTLEKDARLRMELTVKGGKGYVPADRNT
                     DENLPIGNIPIDAVFSPVRKVSYNVTQARVGQITDYDKLTMEIWTDGSVKPDDALAYA
                     AKILKDQLTIFINFEEELEATPEEIQMEPAFNENLFRSVDELELSVRSANCLKNADIR
                     YIGELVQKTEAEMLKTKNFGRKSLNEIKEILAEMGLALGMKYDNFPSREDIERRRKEQ
                     E"
     gene            complement(16577..17203)
                     /gene="rpsD"
                     /locus_tag="PLM79_09815"
     CDS             complement(16577..17203)
                     /gene="rpsD"
                     /locus_tag="PLM79_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOV86648.1"
                     /translation="MARYTGFNCRICRREMTKLYLKGDRCYTDKCAVERRNYPPGQHG
                     QSRGKLSDYGLQLREKQKIRRMYGLGEKQFRSYFKEADRQKGVTGTNFLVLLERRLDN
                     TVYRLGFANSRDQARQLVRHGHILVNGRRVDIPSYLLRPGDAVAVAEKSRTVSVINEA
                     LEAVPRRGLPPWLELEKAKYEGVFKAYPSRDDMTLPVQEQLVVEFYSK"
     gene            complement(17275..17667)
                     /gene="rpsK"
                     /locus_tag="PLM79_09820"
     CDS             complement(17275..17667)
                     /gene="rpsK"
                     /locus_tag="PLM79_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOV86649.1"
                     /translation="MAREKAGARKKKERKNIQNGIAHIRSTFNNTIVTITDASGNALA
                     WASAGSQGFKGSRKSTPFAAQVAAENAGKRAMEHGVQNLEVYVKGPGSGREAALRALQ
                     ASGFNITVIKDVTPIPHNGCRPPKRRRV"
     gene            complement(17680..18054)
                     /gene="rpsM"
                     /locus_tag="PLM79_09825"
     CDS             complement(17680..18054)
                     /gene="rpsM"
                     /locus_tag="PLM79_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOV86650.1"
                     /translation="MARISGIDLPKNKRIEIALTYIYGIGRATSQKILNQANVNWDTK
                     TDDLTDGEINSIRQVIDSHYKVEGDLRTEVSMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPRRSVIGKRKKA"
     gene            complement(18070..18183)
                     /gene="rpmJ"
                     /locus_tag="PLM79_09830"
     CDS             complement(18070..18183)
                     /gene="rpmJ"
                     /locus_tag="PLM79_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOV86651.1"
                     /translation="MKVRASVKRICRKCKIIRRHGTVRVICENPRHKQRQG"
     gene            complement(18225..18446)
                     /gene="infA"
                     /locus_tag="PLM79_09835"
     CDS             complement(18225..18446)
                     /gene="infA"
                     /locus_tag="PLM79_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOV86652.1"
                     /translation="MAKEDSIEVEGTVVETLPNAMFRVELPNGHRILAHISGKMRMHF
                     IRILPGDKVTVELSPYDLTRGRITYRSKS"
     gene            complement(18547..>19104)
                     /gene="map"
                     /locus_tag="PLM79_09840"
     CDS             complement(18547..>19104)
                     /gene="map"
                     /locus_tag="PLM79_09840"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOV86653.1"
                     /translation="HSLCISVNEEIVHGMPSRDRHLKEGDLVSLDFGVVLEGYYGDAA
                     LTVPVGKVSEEARNLCRAAEESLYLGIEQAVVGNRLSDISHAIQEYVEFRGYSVVREF
                     VGHGIGQKLHESPQIPNYGPPGKGVKLKAGMVFAIEPMINQGAPEVEILGDQWTAVTR
                     DRSLSAHFEHTVAVTENGPDILSRP"
BASE COUNT         3967 a         5773 c         5409 g         3955 t
ORIGIN      
        1 gatcatgtcc gccatggggc atccggcatc gggacgcggc aacaggacct tcttgtgggg
       61 attgagaatg gccgcggtct ctgccatgaa cgcaaccccg caaaagacaa tcacttgggc
      121 atcggtagaa gcagcctggc gactgagttc cagggagtca ccggtcaaat ccgcaatatc
      181 ttggatggaa ggaggctggt aattgtgggc gaggatgatg gcccggcgtt cggctttgag
      241 ctgcaggatg cgttgaatca cggattcgtt catgctgggg tttcctattg gatggtgagt
      301 ttcttcttga gcatctcgac atcggccggg gaaagctccc ggccgtcggt gtccaggagg
      361 accgtggaag gcggaatgtc gagctgaaac agcttgggat cgaggctgga gacgactcgc
      421 tgtgattcca gggcgacccg attcgtgttc cccagggcat cgtgactctc gatgatctgg
      481 attcgatagg aatcgagctc gatccagagg tacaacgact gcagggtcgg gtcttccttc
      541 ctgggcacca gtttcaaaac cgccgtctgt ttgctggatt gtttcgaatc caggctgatc
      601 tcaaaatgat tcttcagttc gatcaccccg tcaaaaaggg tctggacgag gggagaggca
      661 aagaaggtgt tggcgtcgta aaaggagatg gtcttttccg aggggacata gagccatgcc
      721 agctggttgt ttgccacaaa actctggggt tccggcttgt cgtattgcca gtgcatccgc
      781 cggggcttct gatagtagag ggtcccggag gcctcggtgc ttgccatgga tcgggcggcc
      841 gatgcggtcg tccattgccg gaagcgcgcc gtgaaggcct gggtttgctg ataatgggac
      901 tccgtcttct tcaaaatttc cttgacgctg agggcagcgg cctcggccga aggaagattc
      961 acccccagca aaaagaccag caagagcccc gcgcctcgcc tcagggcgtt tgttcgcccc
     1021 ttcttcccgc ttcgatccat catgaatccc gccttccaat cacttcccgt ggcttgattc
     1081 cgtccgatac cccgacgatc ccctgctgtt ccatgatctc gatcattcgg gccgcccggt
     1141 tgtatcccac ccgcagccgt ctctgcaaca tggaaatgga ggcctgcctg gtatccaaca
     1201 ccagttgaac cgcttcctcg tatttttcat ccagctcttc ttcggaaagg tcccccgagg
     1261 aagacgcttc ctcgaaacgg acccgatccc ggagagggtc ctccaccagt ttctggctgc
     1321 gccagaattc ggtgagccgt tggacctcct gctcggaaac aaacgccccg tggatccgtt
     1381 gaagcttggc cgtcccggga ggcaggaaca gcatgtcccc ggaccccaga aggctttcgg
     1441 cccctcccgt gtccaatatg gtgcgggaat cgatcctcga ggatacctgg aatgaaatgc
     1501 gcgtggggat gtttgccttg atgatccccg tgagcacgtc caccgaagga cgctgagtgg
     1561 ccaggatgag atgaatcccg gcggcccttg ccatttgagc caggcgggtg atggattcct
     1621 cgacctcccg ggaggcaacc atcatgaggt ccgcaagctc atcaatgatg atgaccatgt
     1681 agggcaggcg gtggtgaggc agtcctgaat cctcggcgct cgggtccgcc cctttggccc
     1741 tgggcttctc cttggagatg atcttgttgt aaccttcgat gttgcgggca ttcttgtccg
     1801 ccaggagctt gtagcgcagc tccatctctt ccaccgccca ccggagccct cgagttgcca
     1861 tcttggcgtc ggtgaccacg ggatggatca ggtgggggat cccttcgtag gtgctcagtt
     1921 cgatccgctt gggatcgatc aggagaagcc gcagatcctc gggggttttt cggaacagca
     1981 ggctgctcaa cagggtgttg atgcaaacgc tcttgccggt tccggtagcc ccggcgatga
     2041 gcaggtgagg catttttgcc aggttggcca ccaccggttg ccccgtaatg tctttgccca
     2101 gggcgatggt caagggggag ggtgaggtca ggtaggcttc ggattccagg acggctcgaa
     2161 tggagacggt ttctctggag tgattgggga tttcgatccc gatggccgcc ttgccgggaa
     2221 tgggggccac cacccgaatg ctgatggcct tcaacgccat ggacagatcg tccgccaagc
     2281 ccacaatgcg gctgatcttg attcccgggg cgggggcgta ttcgtacatg gtgatcaccg
     2341 gtcccggggc aatgtccatc accttgccct ggaccccaaa gtccgcgagc ttttcttcca
     2401 ggattctggc attgtcttcg agtttcttcc aatccggctt gtgattttcc acctcgtagg
     2461 cgtccacaag gtcgagatcc gggaggcggt aaagcccgga cgtcttggga ggggcaacga
     2521 agggagggac ctgccgggaa ggcttcctca ccttgggtgt gacgggaacg gggaggagga
     2581 cttccacggg gggagcgggt cttttgactt ccttcgccct cggagcctcc cggggtaccg
     2641 gagaaaccgg ccgcgtttgc tccggctgcc gggagaacaa ggcggcaatc cgccggactg
     2701 ccccggaaaa gaccttcccg ccccggacgc aaattcttcc cacccaggtc cacagggaag
     2761 ccagggacat gggagtgctg agcagcaggc ccaggagcag ggcgcccacg aggaaaatgt
     2821 gcgcccccag tcggtacagg gacttcagca gcacctcgct caagaagagc cccaggcttc
     2881 ctccgctgcg gattttctct cccccgaaaa caaagagccg cacgtccggc gcctggatgg
     2941 ccagaagggt gatgaggctg atccccacga ggacggcacc gcccacgggc acgtaagcct
     3001 gcggcaggga gagacgtccc cggaacaggc accagctgag tacgaacccg ccgacgatca
     3061 gggcataagc ccccagcccg aagggagtga tcagaagcca ggccaggtgg gccccaaaag
     3121 agcccacccg gttttgcacc gcggggggaa tccgttgaga cgaggagggc gcatcgaagt
     3181 atacaaaatc cgccccatga taggaaatca cgctgaagag ggcaagcagg ctgagaaccg
     3241 ccaggcaaag cgcccatatt tcgtgtttct tgtgtttcac cgaaaaagcc ttccgaccgg
     3301 ggaccccgga tcagatgccc accacgatgg ggtagatgac gggatgccga tccaggaccc
     3361 ggccaaagaa tcgcttgagt tccttgcgga tttccgcctg cagttccgca cagtccactt
     3421 cgaattccgg atcgaggagc atccggtcga acacctcgag gaccacgcac ttggcgtcgt
     3481 ccaggatctc cggtttcgtt ccctcgtgaa tgaagcctct gctgaggatg tcgggaccgc
     3541 tcaggatctc cttggtttcg ttgttgagaa ccagggacgc aatcacgaga ccgtcctcgg
     3601 aaagatgacg ccgatcccgc aggactaccc ccgccacatc tcccacgcct ttgccgtcca
     3661 caaatactcg accggtttcg accctttccc cgacggcggc cttgccgttc ccctgaaaag
     3721 aaatgacgtc gccgttttcc gccaggaggc aattttggga agggatgccc atgatcatgg
     3781 ccaagtgccg atgcttcacc aggtgccgat attctccgtg gatgggaatg aaataccggg
     3841 ggcgtaccgc gttgatcatg gtcttcaatt cctcccggta ggcatgcccg gacacatgaa
     3901 tgtccctcac acgctcatga atgacttccg ccccctgccg gcacaaattg ttgatgatgt
     3961 tctgaatggt gcgctcattg cccgggatga acctcgaaga gaggatgatg gtgtccccgg
     4021 ccttgatctt gagcttcttg tggtcgttga aagccatgcg gctcaaggcg ctcatgggtt
     4081 ctccctggga cccggtcgtg agcatgagga tctgggagtc cgcaaatcga gggagatcct
     4141 ggagggttat gtcttcgccg ggaggaaagt ccagatatcc cagttcccgg gcgatccgca
     4201 cattgccgac catggacttg ccgttgagga ggaccttccg gttgaattcc cgagccagat
     4261 gaatgacctg ctggattcga tgcaggttgc tggcaaagac ggcgacgatg atgcgcccgg
     4321 cgcactcctg gaacacctcc cgcagggtct gtcccacatc tttctccgac agggtgtacc
     4381 ccggtcgttc cgcgttggtg gaatcggaga aaagagccaa aaccccctct tccccgtaag
     4441 ccccgaaccg ggccatatcg aatcgcttgc cgtccacggg cgtgtgatca atcttgaaat
     4501 ccccggaatg gaccagaatt cccacgggag tgcggatggc cagaccaact ccgtcgggga
     4561 tgctgtgaca gacctgaatg aattccaccc caaagggacc cagggagatc ttctggcggg
     4621 gagccacctc gataagcttg gtccgatccg ccagcccgta ctcccggagc ttctcgcgaa
     4681 tgagggcgag ggtcaggcga gtgccgtaaa ctggaatctg aaagtccctc agcaggaagg
     4741 gaatggcccc gatgtggtct tcatgcccgt gggtcacgat caacgcccga agccgttgcc
     4801 gattccgatg caaatacgca aagtcgggaa tcacgatgtc gatccccaac atgtcgtctt
     4861 cgggaaacat cagaccggcg tccacgagca ggagggtatc ctcgtattcg aggaccatca
     4921 tattgagccc gatttccccg aggcctccca agggaatgac cttcagcacc gattccgagt
     4981 cactggcata gctcatactg tcggcccctt tcacctatgc ggttcgaacc cccccatccc
     5041 acctcgccaa atcctctttc gtcatgaccc cgccttcttc ccgtgtcaaa ccacgaagaa
     5101 tggcatcccg cacggcagac atcaagatgt ccttgtcctt gttggtggag ccttcggtgg
     5161 gaatgggact gccgacactg atatggatgg gcccgggttg gatcaaccag gtgcttccct
     5221 tgggcagaat tcgggaagaa ccgctaatgc tcacgggaac aatgggctgt tgtgatttga
     5281 cggccaggac aaatcccccg gccttgaaat ccttcatcac cccgtcggga cttcgcgtcc
     5341 cttccgggaa gatgagaatg gacgtgccgt ttcgaacccg aagagccgcc tcgtcgatgc
     5401 tctggaaggc cttccgacga tccgcccggt cgatgggaat gtagccgcag gcggacatgg
     5461 ccctccccag gatggggatg cggaacagct cctttttggc aaggaatcgg aactgcacgg
     5521 gaagccgcgc aaagagggca aagatatcga accatccctg gtgattggct gcatagatgt
     5581 aagatcggtc gggatcaatg cgatcgagtc cccggatgcg aacccgaacc cccgcggccc
     5641 acaggttgat cctggcccag agccgaccgc accggtgggc ccgatcactc ccccggggga
     5701 ggtgagagac cccgacggcg acacttccca gcacaatcgt ggataccaga agactcccga
     5761 agaacaggaa ggagcgcaac agcttcttca tggagggcag atcctgtggt ttgtttccat
     5821 cgcttttccc atccatcccg gcggttcgcg cagcccgggc tttcggagtt tgagattact
     5881 ataaataacg aagacttgcc aaggaaatcc cggaatattt aaactgtttc cacctgcgag
     5941 tcaacccggc aattcccccg cagcccctgc gggtcccccg gccatcccgt cccatccgcc
     6001 gacaaacgcc gagaacgaaa cgttccgtag aaacgtcccc ggctcccacg cccaagcccg
     6061 gggatggccc ggcccttgtg agggagcggc cgcatgtggt atacggaaga aaggcaagaa
     6121 catcactgcg tgtttcaacg acccacctga gagaagggga agcatatggc aagaggagtg
     6181 aacaaagtca ttctcatcgg ccatctgggg gcggacccgg acatccgtta taccgccaac
     6241 gggacccccg tggccaacct ccgactggca accacggatg ccgtcatggt tggagaaggc
     6301 aagtgggagg aacgtaccga atggcaccgg gttgtggcct ttggaaaggt tgccgaaaac
     6361 tgcggcacct ttctcagcaa aggacggcaa atctatgtcg agggcagaat ccaaacccgg
     6421 caatgggatg accagcaagg caataaacgc tacagcaccg agattgtggc gcgggaaatc
     6481 cagttcttgg gagggggagg aggcggcgaa gaccgctccg ctcaggcgcc ccggggaggg
     6541 ccgtcccctt ctccggccgg aacgccttct tcctattcca accggtctca gaccgaggag
     6601 ctcccccccg gtcctcccgg gccggacgag gacattcctt tctgacgacg gggggtcgta
     6661 cggagagagg ggccggcact gaggaggtct tatcccccaa gccgctccct ctcgaggtct
     6721 cccggaatcc ggttttccgg atgaacgccc tgtcattggg cctcccggcg ccctgttgcg
     6781 agccgtaatc gggcagggcc gccggagaga ggttttggcc gcctgtatcc ctccgtcgcc
     6841 tatggcgccg ggggagacat ggccggagcg ctcagcttgc cttgtccgca ggagcttttt
     6901 cttcgatttt gtcgaccttc tccgaagagg tttcaagggg gtccgaggtg gcttttttga
     6961 aattacgaat ccctttgccc aggccttctc cgatctgcgg gagttttccg gccccaaaaa
     7021 tgatgagaac gatgaccaag attacaagca actccggcat tcctatactc ggcaacatct
     7081 acagctcctt ccttgatgaa tggcctacag gctacccctt gagcaaatcc tccacctctt
     7141 ccaggagttg cagaaattcc ttggactgcc tgtatcgatc cagatgcttc tggtgttcca
     7201 gccaactctc ccacactttc atccaaacgt cattgtgcca gtagaacctc gggccggact
     7261 ctttgccgcc cgtcctggcc cggtactcct catactcgtc gaggcagttc ctgcaaaaag
     7321 ggtatggagt agcaaacttg gaagattcct cggtttccat gtccagttgg gtcccgcact
     7381 tggcacactt gagcatacaa cgagtgcact ggaagatctt cttcaacgcg tcgatcttcc
     7441 tctgacgaag accttgatcg cgacaggact tcaggtcctg aacctttctc ccaaactcaa
     7501 tgatttcggc catcgggtca tcctgtctat ttctcaagat ttttcaggat cttattctca
     7561 ttcttaacac ttgacacctc gtccgtcaag gcggaggaat cgggtttgca cccgttggtc
     7621 aggttgaaca gctgttgggt acaatccgtg aaaagctgct tgctccccct gtgggccttc
     7681 ttcttgagga acatgatggg atcatggagc agcttcttga cgatggactg ggtcaggacg
     7741 tcaatggctt tccggtcctt ttccgtcaag tggggcagat gactgagggt acgaagcagt
     7801 tcctgccttc ggatggcatc cgccttttct ctcagtccca caatggtggg caccacgtcc
     7861 agggtgcaca gccattgttg aaacttgacc gtctcttcgg caacgatatg ttccgcccgt
     7921 tccgcctcca gctgtcttcc ggcccgattg aaatccacaa tgccctgcag atcgtcgata
     7981 tcatacaggt agacgttctc tatttcgttt accctttgtt ccacatcccg gggcacggca
     8041 atgtcgatga agaacaaggg ccggttcttg cgctcacgca ttcgggattt cacatccttg
     8101 tgccggagaa tgggttccgg cgacccggtg gatgtgagga caatgtcgac attcttcagg
     8161 gcatccagaa tatgggagaa ggagacggtt tcccctttga agcgccccgc caattccacg
     8221 gcccgttcca gggtacggtt ggccaccatc agctgacgca ccccctgagc gagaaggtgc
     8281 tccgccgcca gttccgccat ctccccggcc ccgatcagga ggaccctttt gtcccggaga
     8341 tctcggaaaa tctttcgagc gagttccaca gcggcatagc taatggaaac ggcgtgggag
     8401 ccgatcatgg tttcggagcg gactcgcttg gccacggaaa aggctttatg aagcaggcga
     8461 ttgagaatca cccccaccgc cttgcactcc gttgcttccc ggtaggccgc cttgatctga
     8521 cccaagatct ggggctcccc caccaccatg gaatcaaggc tggaggcaac tcgaaacaga
     8581 tgcttcaccg cgtccagaaa ctgataggtg tagaaacacg attccacctg ggcggagaga
     8641 gcatcgccca aatggccccg aaggagagac ttgacctcgt gggtggcctc atgcagctgg
     8701 ttgcacacga acagaatttc cacccggttg caggtggaca gtacgaacag ctcgtccaca
     8761 taacgaagct gcgggaaccg gtggagcaga tgtttgtcct cccgacacac tgcggccagt
     8821 ctttcccgga tttcaatcgg agcagtctta tggttcattc ccactaggac gagttgagac
     8881 atatcccctc tcacctgacg aacacggtgt gatgcccctg gaggaccaga ttgaccccga
     8941 ggaaggtcac cagcaccgct gaaaacccga agatggccag ccatgcggca cgacgtcccc
     9001 gccatcccac cgccagtctc tcatgaagca gtatggcgta aatgaaccag gtaatgaggg
     9061 caaaaatctc cttggggtcc caattccagg gagatttcca aacactgccc gcataggcga
     9121 accctgtgat gagccccagg gtcatgaggg gaaaaccgaa aaccagacag acatagttga
     9181 gcttgtccag gctttccaag gacgggagac gcaggtacag gaacccgaaa ttctttcgtt
     9241 tgatcgttcg ttcctgcaag aggtacatga tcccggcgct gaacgccagg gcgaacaagg
     9301 cattggccag aaacaaggcc gagacgtgca ccaccaccca gacactttga aagatcccgc
     9361 tggtgggaat catccgggcg ggcaccaggg gagaccccag cataaacaaa acggccaggg
     9421 gcgatacaaa ggaacccagg attcgcaggt gaaaccgggt ctgaaccacc aggtacacaa
     9481 ccaccaagac ccaggcaaag aaagaaaggg cttcctggct gttggtgacc gcaaggcgcc
     9541 ccatggaaat cgagcgcatg atcaaggcca ggccctgcaa ggtcgctccc gccagcaaga
     9601 tgcccgaaga caccctgtga atggcgtcct gatctcggac caggtagatg acgtatccga
     9661 tggccccaac gcaatacgcg atggtcgcga ggatcaggaa gacggtttcc atagttcatc
     9721 ccaaatccgg ctcaattctt cccggtgaat ccagccgttg cagacttcca acacagcatg
     9781 aaatgcctct tctcttcttc cttcccgaat ccatgcggga agcggaaggt tcgacacctt
     9841 ccggaaaagg cgctgctttt cgtcacttcc caacgacttg gacaggatca acttgcgaag
     9901 tcgtcccatc cactccagaa acgatgccca ttcggggccg aattctccct cgagcttttc
     9961 ccggatttgc ctggccacgg cagggctcag ccccgtggtg ctgacggcga tggcaagagg
    10021 cccccgctcg atgagcgagg ggacgatgaa cgatcccatc tcgggtccgg tcgccgtatt
    10081 gcaccacagt cttcgctcgc ctgcctgacg ggcgacctgc tcgttcaaac cggcatcact
    10141 ggtggcggca aagaccaggt ccgcctcctc cagcagatcc tcccgatagg tctctcccag
    10201 gtattggacg cggccatccc ggacccattc ccacaggccc gaagtcaatg tccgagccac
    10261 gacccgaatg gcggccccgc tgggtaggag acctcggatc ttgcgttcgg ccaccgtccc
    10321 accccccacc accaggcaga cccgcccttc caaggacaca aacatgggat aataggaggg
    10381 gctcaccggg aaagcccccg atggaagccg ggaacgagag accgaaggcc gctctccacg
    10441 ggtggtttca cggcattgga cggaacggtc atcttgcgct cctttccccg ggaaagccga
    10501 ccccgccaaa gttgtccctc acatccttga tttcataggg ccttgaaatc taacacgaac
    10561 ggcaggtgga aacaagcgag gaactccaac gcggatttcg ccggcaaagg ttgatcctct
    10621 ccccacgaat ccgggacctt tcggcaatcc cccgcgaccg atcaaccccc cgagcccaac
    10681 ccgcgcaggg ttcggcgccc cccggaaggg ctccccagca ggaggcggcg gccgccggac
    10741 caagccacgc gcgtccccct atgcctcctc gggccccttg caccaacggt ttcgggacga
    10801 acgggcttcc ggacggaaat tagactcttc gatagatgac gtaatccgcc atgtcacaca
    10861 agagggtctt tgtgggattg gaggggaaaa tatgaagagc ggctttcgcc ttctggacat
    10921 attccatggc caaccgctga gtgtattcaa tgccgccgga ccgaatcacc aggttcctga
    10981 tttcgtcaaa ggcttcctgg gtgatttcct ctccactgaa aatctcgcga agacgattct
    11041 tctcggcgga acggccgttc atcaaagcac agatcagggg cagtgtggcc tttccttctc
    11101 gcagatcgtt gcccacgggc ttgcccagtt cgttcgggct gcccatgtag tccagggcgt
    11161 cgtcgatcag ttgaaacgcc agacccaggc tgagtccgaa agagcgaagg gccgactctt
    11221 cctcggcccc ccctccgccg aaaatggccc cgatatggca cgcggcactg atcagaaccg
    11281 ccgtcttccg ggtgatgatt tccaggtatt ccttttcctc cacctccagg tcgtccgatc
    11341 ggaccatctg gagcacttct ccctcggaca tgagggtggt ggcctgggac agaacttcca
    11401 ggattctcat attgtcgtag ccgacggtca tgagaatggc cttggaatag agaaaatccc
    11461 ccaccagaac cacggcggga ttgccccaga tggtgttggc agcgggcttg cttcggcgca
    11521 gttccgcatg atccaccacg tcatcgtgaa gcagggatgc cgcgtgaaga aactcgaaaa
    11581 ccacggacaa aggaacgtct cgattccccc tgtagccgca caggcgagcc gacaggatca
    11641 tcagaagcgg gcggagtctc ttgcccccgc tgcggaggat gtagcgcccc accacggaaa
    11701 tgagaggaac actcgtcgtc agcaagcggt tcatctccgt ctcgatccgt tcaagatcgg
    11761 gccgaatcct tacaagaatc cgctccttga tgtcctcgat ctcgggttgg aacagtttcg
    11821 gcaggacggg actttcaccc ggtaaagtgc ttatttggtc catttgcaca attctaacgt
    11881 atggttgttc agcctacgcc cccgtggtcc tcgcccccgc tggatacagc gagggtccat
    11941 aggtacatag ccaaaatttt ctcatctgtc aaagcgtttt cgggtcccct tccccgggcg
    12001 aagctcccgg cctggcagcc ccgaaccggc agaccccggc ggccggatca ccgggtccgc
    12061 cagttccgcc tccaggtcat aatcgtgggc ggccgtgatc ctggccatgg ccaattcccc
    12121 gggagaggcc gttccccggg tgatgagcac cattccgtcc tgctccggcg cctgggtcgg
    12181 cagtctggcc cccagcagga gttcggtttc cggatgatac ccttccacca ggaccgggag
    12241 gaccttcccc acccagcgtc tcaaccgacg ccgggatatt ctcctctgcc gttgaagcaa
    12301 cgcccgtttc ctcttctcct tgatccggct ttccaaggga tgggaatacc gtgccgccgc
    12361 cgtgccgggt tcgggagaat aggcaaacac ccctgcatgg tcaaactggg cgacttccag
    12421 gaactcgaag agctctcgaa actcctcttc ggtctccccc ggaaatccca ccatgaggga
    12481 cgttctgagg gaaatgtggg ggaccttcga acgaatgcgc cggatgagac tcagggggtc
    12541 ctcggcagtt cttctcccca tccgttcgag gaccccggcg gaacagtgtt gaatcggcac
    12601 gtccaggtaa ggaaccgttt tgcgagactg agccatccaa tccaacaaat catccgtcac
    12661 tccgtccggg taggcgtaca ggaggcgggt ccactcgatc ccctgcacct tctccaaggc
    12721 atcgaggagc ggcaccagcc gggcggtccc tccctgatcg gtgccgtagg ccgtcgtgtc
    12781 ttgggcaatc aagttgattt ccttgactcc ttcgtccgcc aaccgggccg cctccgccac
    12841 caggtccgag atcccgcggc tgcgataacg ccccctgagg tgaggaatga tgcaaaacga
    12901 gcatgaatgg ttgcagccct cggcgatctt caggtaggcg gtgtggggtg gggtcgcccg
    12961 tatccggggc gaatcggagc ccaggaggtg tcgaggcgtg gaaatccaga ctcgaagccc
    13021 gttcccccgt tgccgctcgg ccaaaatgtc acccaatcgg tgatagtggg aggttcccag
    13081 gaacaggtcc acctcgggaa gaagctctgc cagctttttc ccatagcgct ggaccacgca
    13141 tccggttgcc accaggcatt cacaggaacc ggccgatctg aatttcgccg cctccagaat
    13201 ggattgaacg gcttcttcca cggcgctctg cagaaaaccg cacgtattca ccacgataat
    13261 cgatgcctcg gccggatctt cggtcatggc atagcccgaa cggaccaact gggggaccat
    13321 ggtctcactg tccaccaggt tcttcacaca acccaagctg ataagggcag ccaagggctt
    13381 catcccacct tcttccttca tatttcccga tcgaggacct gccgtgcctc cacggatcac
    13441 cgtgagaaac tcgccaccgt aaagagatcg atcccgtgga agatcagcag atcccaagcg
    13501 accatgcccg cccccagaag aaaccgtaga cggaatcgcc tttgcccaag accttcctcc
    13561 ccgatccttc ggcaaagcgc aaggaaacta ctggtcatca cataacattc cacagcgaaa
    13621 ggaggaaacc cgagaaaacc caggataggc atctcaaaga ttttgagccg gcccacatgg
    13681 ggaatggtgt actgccactt ggttcccgcc caaaaattcc agaactccca gaggaaaccg
    13741 cataccaacc ccgcggccag gaggagatag aaggtgcgaa gagagccttc ctcccattgg
    13801 cggagaagcg acggcgctcc cagacggtaa ttgatgggct ccaacaagaa gacgaagcct
    13861 ccccagacca ggggaaagaa gtgccgaggc caaatgagag ggaggagcag aaacagtccc
    13921 cccaccccca agaaaacctg gtgaaaccgg ggagacggac ctccctttgc cggtctcccg
    13981 ggctcgagaa ctcgaaggtg gtccaggagg ctttgggtgg tgaagatccc ggggatgacc
    14041 gtggcgaagg aaacggcata cccgacccac cgggtgaagg tgccgggggg cacattatgg
    14101 tattcccagt tgtttaagcg aaaattccag gtttcgaaga cgagccaaag attgatggag
    14161 aaaagggaaa gaaggaagaa cctcgatggc cgacggaaaa gcatcgagtc tccccccctg
    14221 cgatgcagga gagattgaaa aaagaggatg tagctccacc aagcgaagca gtaataccag
    14281 gtccaatagg gctccacctt gcggctcatc tgaaggccgg cccagagaaa aaccaccaat
    14341 cctgcggcca tacccgccgt gggcaagaga cgctctggaa accaacgcac gacttcctgg
    14401 cctcccctgg aggtccttcc ggcggaaagc acccgttccc aaaagtctca catggcctgc
    14461 cgaaagggat cgaaaggctc gggaagggtg tccgtctttc cccggacggg aaaatttttc
    14521 attgccattg atttccgatc attttgcgct taacaatata aagtttaaag tgtcttgatt
    14581 tcccggagga ttccaaccca acaaaggagt gagcgggaat ggcaaaagag agagtctgtc
    14641 gaggacacgc ggcgcaatca gaaagaaacg ggaaaaccct cgggcctgcc atgtgcaagg
    14701 aaggcaaggg cgtccgcaat ttcgcatgtc agaactacga cctctgcctg gatagagccg
    14761 ccaaggccat gtggagcggg ttcacctgca gggactgcgc ccattattac cagaaagagg
    14821 aagaggccac cccgtgatcc cccgagattc ccccaagggc ccaacgccaa cgccggcata
    14881 gagccggaag ctcccaaccc tttcgaagac cgaagcaaac aacccgcgtc cgccgcgggt
    14941 tgtttgcttt ttcttgaaac ctccatggaa tcggagaacc ggaatcactg cccctcgacg
    15001 ggcggcgctg gggccgcagg ccgggtctcc gcgggcgata ccttgggttg cgccgctttt
    15061 gccttggcct tcttgcgtcc ttccttgggc tcagtcatca attccacgat gcacatggga
    15121 gcggcgtccc cccgccgata ccccaccttc actatacggg tatagccccc cggccgattc
    15181 tgatagcgcg gcgccagctc cgcaaacagc ttgtgcacca cgcttttgag cggaatggtc
    15241 gccaatgcct gccgcctggc atgcagatcg ccccgcttcc ccagagtgat catccaatcc
    15301 gcccagcgac gcaattcctt ggccttctgc accgtcgtgt agatgcgctc atgctggagc
    15361 aaggaagtca ccatgtttcg gaacatggcg gatcggtggc tcgttgttcg attgagtttt
    15421 ctgcccgaga ccccgtggcg catggctatt cctgttcctt tcttctgcgt tcgatatcct
    15481 cacggcttgg gaaattgtcg tatttcatcc caagggcgag tcccatttcc gccaggattt
    15541 ccttgatttc gttgagggat tttcggccga aattcttggt cttgagcatt tcggcctcgg
    15601 tcttctgaac cagttcgccg atgtaccgga tgtcggcatt cttgagacaa ttggccgacc
    15661 gcacggaaag ctccaactcg tccacactgc ggaacaggtt ttcgttgaag gcgggttcca
    15721 tctggatttc ttccggcgtc gcctccagtt cttcctcgaa gttgatgaaa atggtcaact
    15781 ggtccttgag aatcttggcg gcataggcca gggcgtcgtc cggcttgacg ctgccgtcgg
    15841 tccagatctc catggtcaac ttgtcgtagt ccgtgatttg ccccacacgg gcctgggtca
    15901 cgttgtagct caccttgcgg acgggagaaa acaccgcgtc gatggggatg ttgccgatgg
    15961 gcagattttc gtcggtattg cgatccgcag gcacatagcc cttgccgccc ttgaccgtca
    16021 gctccattct gagcctggcg tccttttcca gggtgcagat atgctggtcc ggattcaaca
    16081 cctccacgtg agcatccccg cggatgtccc cggccttgac ggcaccctct ccctgtttat
    16141 cgatcaccac gagcctggta ccgtccccgt gcattttgag ccgcacttcc ttcaggttga
    16201 ggatgacgtc ggtaacatcc tcgaggaccc cgggaatggt ggagaactcg tggaggacgc
    16261 cttcgatctt cacgctggtg atggctgaac cctgaagcga agaaaggaga acccggcgca
    16321 aagcgttgcc cagtgtgacc ccgtatcccc gttccagggg ctcacacacg aacttggcgt
    16381 aattgttcgg tcgatctttg gtttctactt ccaagcgctt tggtttgatg agttcgcgcc
    16441 aatttctttg catgttttgt cctcatccaa agatgattgt cagggcaatc gtcctctgtc
    16501 ttgaggtgag agaatgaacg ctcacggccc ccaaatccga agggtcttac ggaggaagcc
    16561 caggttcggc agcgtgttac ttggagtaga actccaccac caactgttct tgtacgggca
    16621 gggtcatgtc gtctcgggac gggtaggcct tgaaaacccc ttcgtacttg gccttctcca
    16681 gttccagcca gggcgggagc ccccgccggg gaaccgcttc caatgcttcg ttgatcacgg
    16741 agaccgttcg gctcttttcg gcaacggcca cggcgtcgcc gggccggagc aggtaggaag
    16801 ggatgtcgac ccttcgtccg ttgaccagga tgtgaccgtg tcgcacgagc tgtctggcct
    16861 gatcacggga attggcgaat cccagtcggt agacggtgtt gtccaggcgg cgctccagca
    16921 ggaccagaaa gtttgtgccc gtgacgccct tctgcctgtc cgcttccttg aaatagctcc
    16981 tgaattgctt ttcccccagg ccgtacattc ggcggatctt ttgtttttcc cggagctgga
    17041 gcccgtaatc cgacagcttt ccacgggatt gcccgtgctg cccggggggg tagttgcgcc
    17101 gttccacggc acacttgtcc gtgtagcagc gatctccctt caggtagagt ttcgtcattt
    17161 cgcggcgaca gattcggcaa ttgaaaccgg tataacgagc caaggtactt cctcctcttt
    17221 gggacgggct aacggagcat tctcatgatc caacggactg acgggcgcac gggattagac
    17281 tcgcctccgc ttgggaggtc gacaaccgtt gtgggggatc ggagtcacgt ccttgatgac
    17341 ggtaatgttg aatccggatg cctggagggc gcgcagggcg gcttctcttc ccgatccggg
    17401 tcccttcacg tacacttcca ggttctggac tccgtgctcc atcgcccttt tccccgcatt
    17461 ctccgcggcc acctgagccg caaagggagt gctcttccgg gagcctttga aaccctggct
    17521 tccggcactg gcccacgcca atgcgtttcc gcttgcatcc gtgatggtta cgatggtgtt
    17581 gttgaacgtg gagcggatgt gggcgatgcc gttctggatg ttctttcgtt ctttcttctt
    17641 tcttgctccc gccttctccc ttgccatact tcctcccgat tacgccttct tgcgcttgcc
    17701 gatgacggat ctgcgaggcc ccttgcgggt gcgcgcgttg gtatgggttc tttgcccgcg
    17761 gacgggaagg ccccgtctgt ggcgaagccc ccggtagcat cccagatcca taagccgctt
    17821 gatattcatg gaaacctccg tccggaggtc cccttccacc ttgtaatgag agtcgatgac
    17881 ctggcgaatg gagttgattt ccccgtcggt gagatcatcg gttttggtgt cccagttcac
    17941 attggcctgg ttcaggatct tttgggaggt tgcccggccg atgccgtaga tgtaggtcag
    18001 ggcgatctcg attcgcttgt ttttgggtag gtcaatacct gagattctag ccaaagtccg
    18061 tccctcccgc tagccttgac gctgcttatg tctggggttt tcgcaaatca cccttacggt
    18121 gccgtggcgc cgtatgatct tacactttcg acagatccgc ttgaccgatg ctcgcacttt
    18181 cataccctga cactcctttt cttcctgcca cggacacaag acggctagga tttcgagcgg
    18241 tacgtaatcc ggcctcgggt caggtcgtag ggggaaagtt ccacggtgac cttgtctccg
    18301 gggaggatgc gaatgaagtg catgcgcatt tttccggaga tgtgggccag aatacgatgt
    18361 ccgttgggaa gctcaacgcg gaacatggcg ttgggaaggg tttcaaccac ggtcccttcc
    18421 acttcgatgg agtcttcttt ggccatgcct ttcaccttct ccttgccgtc ttctctcgaa
    18481 gggcttgtag aaattccgcc ggaacaaaaa attgtattcg attccccgat tcctgtaaag
    18541 atcaaactag ggcctggaca aaatatccgg tccgttctcc gtgacggcca ccgtgtgctc
    18601 gaagtgggcc gaaagagatc ggtcccgggt caccgccgtc cactgatccc cgaggatctc
    18661 cacctccggc gccccctgat tgatcatggg ctcaatggca aacaccatcc cggctttcaa
    18721 cttcacgccc ttgccggggg gcccgtagtt gggaatctgg gggctctcat ggagcttctg
    18781 cccgatgccg tggcccacga actcccggac caccgagtag ccccggaact ccacgtattc
    18841 ctggatcgca tgagaaatgt ccgaaagccg gtttcccacc accgcctgtt cgatccccag
    18901 gtacagggat tcttcggccg cccgacaaag gttccgggcc tcctcggaca ccttccccac
    18961 gggaaccgtc aaggcggcat ccccgtagta cccctcaagc accaccccga aatccaagct
    19021 gacgaggtcc ccttccttca ggtgcctgtc ccgggaaggc attccgtgca caatctcctc
    19081 attcaccgag atacacaggg agtg
//
LOCUS       DAOOVC010000065        18798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2007_length_18798_cov_6.915115, whole genome shotgun sequence.
ACCESSION   DAOOVC010000065 DAOOVC010000000
VERSION     DAOOVC010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18798
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2007_length_18798_cov_6.915115"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PLM79_09845"
     CDS             complement(<1..231)
                     /locus_tag="PLM79_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HOV86654.1"
                     /translation="MATYGLKLFAGNSNPELAQQICSNLEVSLGRALVSTFSDGEIRI
                     EIQENVRGADIFVVQSGAHPVNDHLMELLVMID"
     gene            complement(599..1345)
                     /locus_tag="PLM79_09850"
     CDS             complement(599..1345)
                     /locus_tag="PLM79_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOV86655.1"
                     /translation="MLEQLRYLIQYQILEDKKAALIRSCEDAPARVSELEKEFEHFEA
                     EYLSRKAEYEHAKKMHRAAEQTIADLEARIQRSKRRMNEVKTNKEYQAILKEIEEIKK
                     EIGVNEDAVLGFLETIETLGRELKARSKELEERRKALEQDKAVLKAESDKLKERLDRF
                     EAAQEQVREKLKPDLRKRSENLITRQAGIAVAAVENGVCQVCRLNIPPQKFIELQRDE
                     SIMQCPHCHRFIYWPGHEAYSVPEEETENL"
     gene            complement(1413..2237)
                     /locus_tag="PLM79_09855"
     CDS             complement(1413..2237)
                     /locus_tag="PLM79_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOV86656.1"
                     /translation="MVRVEDLLTWLDAHAPFRYAESWDRCGLQVGDPHMPVRRVLVAL
                     DPSSGTFFEAQQHECQCLVTHHPLLFQPLTQVRPDQYPGNLLARAIRNEIALVVAHTN
                     LDVSREGTNDQLAKLLELRDPDPLQVNPRWEGEAQYLGMGQVGNLCRAVSLDELVDRL
                     RKLFGSADLRVVGTPAGKIHRVALCTGSGGNLVDLAVSRGAHCYITGDLKYHEAQKAI
                     EAGLVLVDVGHFASERIVLEPLAKYLESQAKTRKLELEVLESGLERDPFTTHRGFG"
     gene            complement(2431..2503)
                     /locus_tag="PLM79_09860"
     tRNA            complement(2431..2503)
                     /locus_tag="PLM79_09860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2468..2470),aa:Ile,seq:cat)
     gene            complement(2575..4350)
                     /gene="rpoD"
                     /locus_tag="PLM79_09865"
     CDS             complement(2575..4350)
                     /gene="rpoD"
                     /locus_tag="PLM79_09865"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOV86657.1"
                     /translation="MKPRRDPALAKTEMDDLKRLITVGKEKGYLTYDEVNEALPDDLV
                     SPEKLDDMMMIFDEMDIEIVDSEQQVQVVRESAGADSECLMEEEEDELQIDADSASRV
                     TDPVKMYLREMGQVSLLTREGEVEIAKRIEMGEREVFNAIMESSIGIREILSLKERLE
                     NSVVRLNDVVREMDEEITPEEEEIQKNRIIGMLENVQRLDEANKIMHMTLLREDTTAE
                     DAQGLRQMVQQNKEQIVNLLKGLQLGRRHIDSIIDKLRLYLRRIEKSERDLEECLLRS
                     GKPFQDLKPFFERPQDEMESYARQMGIQQQEFHDLVCRAVEAKDRIGTLEMEAKMDSK
                     TLREILRRVEEGMLRAKLAKSELIEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMK
                     AVDKFEYQRGYKFSTYATWWIRQAITRAIADQARTIRIPVHMIETINKLIRTSRYLVQ
                     ELGREPTPEEIAEKMEFPVEKVRKVLKIAKEPISLETPIGEEEDSHLGDFIEDKRVAS
                     PVDAVINLNLSEQTRKALATLTPREEKVLRMRFGIGEKADHTLEEVGQDFAVTRERIR
                     QIEAKALRKLRHPSRRKELKSFIES"
     gene            complement(4380..6215)
                     /gene="dnaG"
                     /locus_tag="PLM79_09870"
     CDS             complement(4380..6215)
                     /gene="dnaG"
                     /locus_tag="PLM79_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOV86658.1"
                     /translation="MRDRLISVSASDTAALVKQAVDIVDVIGQVVPLRRTGKKYTGIC
                     PFHPEKTPSFQVDPENQLYYCFGCGSGGDVLSFIMRQQNLVFGDAIRYLADRYHINLP
                     RKDPSSNLSPEALASVRKERDRLYAVMELAGDYFHQNLVHARTGRDARDYLKNRAVPE
                     ELIRREKLGYAPPQWDSLARYLRKSGVNPEIAVTAGLLSKSSKNPDQLYDRFRNRIIF
                     PIRDERDRVVAFGGRILGGSAPDEPKYLNSPETPIYQKGRMLYQMARAREACRRERKI
                     VLVEGYMDLLAFHAHGFERVAATLGTALTPPQVRLLARAADEVILAYDGDEAGERAML
                     RALPLCLQDNLAVSFLQFPDGMDPDDFLRANGASSLEALMNTRQDLGLCAIRKALKGW
                     DGRTSTKAQIISDLAPIFEALHQPVLRSEYLRVISEGLALPESVIERQLRHGRSHPAR
                     WASPVAPVSPCPLPESREEKILQLLIHHPEVLEQVRSSGAADCFREPRVRKAVQVLLD
                     LPPNRLASLKSSELYDLFADPDLRQLVTRLVMESADLEEPIVQLRDWLDALTRRSLKE
                     ERRRLKEALAQAETAGDATQVRRILLQIQKLGSVSNSVKHSPDNV"
     gene            complement(6311..6760)
                     /locus_tag="PLM79_09875"
     CDS             complement(6311..6760)
                     /locus_tag="PLM79_09875"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOV86659.1"
                     /translation="MMDLMSRIEQALKEAVRKKNEDQKNALRLLLTAVKLKEKELKRL
                     PNDAEVQQIISTLIKQRREAAEQYLKGNRQDLARHEEEEILLLRGFLPEALSPGELEK
                     MVLEAVAETGAQSLKDMGKVMKVLMSRVAGRADGKEVNELARKKLGG"
     gene            complement(6757..7524)
                     /gene="hisA"
                     /locus_tag="PLM79_09880"
     CDS             complement(6757..7524)
                     /gene="hisA"
                     /locus_tag="PLM79_09880"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOV86660.1"
                     /translation="MIIFPAIDLKDGLCVRLMQGDPERATVYGTDPVETARRWEDQGA
                     RWLHVVDLDGAFSRVPKNFQVVASILRAVSIPVQVGGGIRTLETMEEYLSLGVRRVIL
                     GTVALREPALLEEASRLFPRRIAVGIDARDGMVAVEGWKETSRTDAVQLVRTLDVLPL
                     SAVIYTDIHRDGMRSGVNVEATRRLMEATRLPVIASGGVATLGDIEALLPLIPLGLEG
                     VITGKALYSGTLDLRDALEFLQSRAAASVGEKPKGGQ"
     gene            complement(7583..8074)
                     /locus_tag="PLM79_09885"
     CDS             complement(7583..8074)
                     /locus_tag="PLM79_09885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOV86661.1"
                     /translation="MREQSKPDMAGKVDPDIRASESREVHPQISEASPAAQATIRVLY
                     GDTDAMGQAYYGNYLKWFEVGRAEWFRSRGMTYRELEGGGIFLPVVEAHCSYHKPAFY
                     DDILIISTRFRFVGPIRLRFEYEIRRDPLTLASGYTVHVCVNRDRKVLKPPHYLKALL
                     TKE"
     gene            complement(8099..9397)
                     /locus_tag="PLM79_09890"
     CDS             complement(8099..9397)
                     /locus_tag="PLM79_09890"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOV86662.1"
                     /translation="MKVALEDWRTIILVGDGMGDYPLEELRGKTPLEAAHTPTMDGLA
                     ARGLMGLARTIPASMEPGSDIANMSLLGYDPLRYHTGRAPLEAASMGIRLGPDEVAFR
                     CNLVNLEEAPSGMRMGDYSAGHISTPEARELIRALQEEAVLGPLRLYPGVSYRHLLVW
                     AGGNPDLRTTPPHDIPGEPVGPYGEVYRREPVLDSFIQRAAQVLANHPVNQRRQEAGK
                     RTANGVWLWGQGKAPSMPTLREKFGIVGTMISAVDLLKGLGVYAGLDPVPVPGATGYL
                     DTNYRGKVQAALEAMEAGHLVYVHIEAPDEAGHEGSVAKKIQAIEDFDEKVVGPVVRG
                     MEKFPRVRLLVVTDHLTPVHKRTHVSDPVPFLLVSHLNDGKESTGPPATFCEKSAART
                     GIKVASGEELFGLLMQPTSSAPPEQAKNRRGFFPPGKPAP"
     gene            complement(9406..10728)
                     /locus_tag="PLM79_09895"
     CDS             complement(9406..10728)
                     /locus_tag="PLM79_09895"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOV86663.1"
                     /translation="MRSIGIGLIGWGTVGCGLIRILRENAALMENRLGTRLELRRVAD
                     LDLNRPRPVQADPKILTTRADDILEDPDIDVVVELMGGIGAAKQIISRALQNRKHVVT
                     ANKALLAHAGNELFALAREMGRSIQFEASVAGGIPFIKSLREGLAANRIQTIFGILNG
                     TANYILTRMTDEGLSFQDALKEAQARGYAEADPSLDIEGTDTAHKLSIAGAIAFGTPI
                     QFDQVYIEGISKIDPVDIRFGEEFGYQLKLLAIARNTDDGIEMRVHPTLIPNQHVLAS
                     VKGAYNAVHIHGNAVGNIMLYGLGAGMMPTGSAVAADLMDLARDIQLGVPTRIPPLAF
                     PEDRISPVPVKPMADVVTCYYFRFAAVDRPGVLSKISGILGQHDISIAAVIQKGRQAE
                     ETVPIVMLTHEAREADVRNALEEIDRLEVVHAPTQFIRIENQETDQMA"
     gene            complement(10880..12067)
                     /locus_tag="PLM79_09900"
     CDS             complement(10880..12067)
                     /locus_tag="PLM79_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV86664.1"
                     /translation="MSFKIAERSRIHRLPPYVFAQVNALKMERRRAGEDIIDLGMGNP
                     DLATPKHIIDKLVEAVAKPHNHRYSASRGITKLREAIARRYKERYDVDIDPESEAVVT
                     IGAKEGLSHLVLVLISPGDVVFSPNPTYPIHPYSAIIAGGDVRSIPISPDSNFLEDLE
                     KATKQTWPRPRLLIISFPHNPTTAVVDLEFFRKIVDFAKENRIMVIHDLAYADLTFDG
                     YRAPSFLQVPGAKEVGVEFFSMSKSFSMAGWRVGFCVGNSEMISALTRIKSYLDYGVF
                     QPIQIAAIIALNGNQSCVREISEVYRVRRDVLVDGLNRIGWHIEKPRGTMFVWARIPE
                     RFRDMGSVGFSKYLIEEAKVAVSPGLGFGEYGDEYVRFALVENEKRTRQAIRGMRKAL
                     QEK"
     gene            12326..14254
                     /gene="selB"
                     /locus_tag="PLM79_09905"
     CDS             12326..14254
                     /gene="selB"
                     /locus_tag="PLM79_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOV86665.1"
                     /translation="MKQVILGTAGHIDHGKTSLIRALTGIDTDRLKEEKLRGITIELG
                     FAYLDLPGGERMGIVDVPGHERFVRHMVAGAAGIDLVALVIAADEGVMPQTREHMEIC
                     DLLRVKKGLVVLTKTDLVEDPEWIEMVKEDVADFLKGTFLDGAPVIPVSAVRGTGMDE
                     LKEQLAVLYREVEPRSPEGPFRLSVDRVFTMRGFGTVVTGTSLSGRLRVGDPVTVYPS
                     LHKTKVRGIQVHGEDVEEVLPGQRTAINLQSIERGLIRRGEVIASPGALVATHMVDVH
                     LDLLSSSPRPLKNRAKVRFHTGTAEHLAVLVLLEQEELAPGGETFAQIRLDTPIAALR
                     GDRFVLRSYSPVRTIGGGTILHPFPGKHKRQEKRKAAVALKELLLANEPDAALWHVLH
                     SGPGGITEEELRVRANIPPKSLDKILQQFTSQKRVIVVDKDNRRLIHPDVLQELKGTV
                     LEALTEFHTQFPLKTGMAKEELSARLPRSVDTRLYNFILRQLAEQDLVVQEMEWVRLS
                     SHKTDLSKEEESIREKIEKAYKDAGLQPPFFKEVAGTLPGNSRRHQDVLEWMFARGTL
                     VKTKEDLYFHASALKELQDRLVSFLREHGEISTPQFKDMTQASRKYTIPLLEYFDSQK
                     ITIRIGDVRRLRESRAGG"
     gene            14293..14586
                     /locus_tag="PLM79_09910"
     CDS             14293..14586
                     /locus_tag="PLM79_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOV86666.1"
                     /translation="MLKHVVFMKFKPGVTEEEIGDLAGSLGKLPGVVPEIQGFEFGRD
                     LVRSERSYDFALVSTFADLDAMNRYQVHPEHQRVVAKVRQLSQSVLAVDFETR"
     gene            15082..15693
                     /locus_tag="PLM79_09915"
     CDS             15082..15693
                     /locus_tag="PLM79_09915"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86667.1"
                     /translation="MVVGLFLLTAVPGFVPSARADSSSPFELVRSGSDRGLRIIKSSL
                     FEGGPGLQQRRDEILRIVEEYFDFDEIAKRALGRPWKDLAPEKRQEFVRLFKELLFDT
                     YVSRIEATARPDTGIRYDRQSVEGRYAVVKTWVTNAGRPDFQIDYRLLLSGGQWKVYD
                     VVIEGISLVGNYREQFASILTNESFDALLKRLQEKVRTRSPLQ"
     gene            15932..16252
                     /gene="sugE"
                     /locus_tag="PLM79_09920"
     CDS             15932..16252
                     /gene="sugE"
                     /locus_tag="PLM79_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HOV86668.1"
                     /translation="MAWVILIVAGLFEVGWAIGMKYTEGFTRLWPTMWTVPAMVLSLW
                     LLGIAMKSLPVSTAYSVWVGVGSVGTVILGNVLLGEPVNAARLISVGLILAGILGLKL
                     ATPA"
     gene            16657..>18798
                     /locus_tag="PLM79_09925"
     CDS             16657..>18798
                     /locus_tag="PLM79_09925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV86669.1"
                     /translation="MRPRIMPRLFSSLRFQLIAIVLLSVLPAIALILYSGFEERRNAA
                     LHARTNTRRLAELASIQQARFIDEAYRLLLTLSESPHLYSSDAEQCSAYLLDVISWHP
                     HYLNIGVLGTDGRVLCSALPVESRMPLPNEDFFRHALENEGLVIGSAHSRDDSGKASF
                     ELGYPVHDSAYGVRGVAFAAVDLAWLSDLAAYLGFPEGATLALTDRDGTVIVRYPDPE
                     RWVGKVVSNKAIVKTALEQGKGTMEAVGVDGVNRLYAFNVLGDFAQPVILYTGIPTST
                     VFAEANRALTRNLITLGIVTILALIVAHVLGHLVVMRRVNALVRATDRIGRGELGART
                     GMSSGNSELEQLGTALDAMAESLERKGLERDEMEAALRRSEERYRALVEQIPVVTYTA
                     ALDEARTILFISPQVECLLGFDEGDFYEDRDTWRKRLHPDDRERVLRESSRCRKENDL
                     FICEYRMLTYHGLVKWIRDEAAVVLDKAGEPLFIQGVMTDITEQKRSEEELVEAHKEL
                     EIRVAQRTEALARANEELRQSAETLKSFAASVVHDLRSPAIGAYGLTKLLWKQYGDVL
                     DEKGTHYCDQIMKASEHVVELAEKINVYMATKETPLSIETVSLGELLGTLRDEFSSRF
                     SARAIRWVQPERDVEVRADRLSLLRMFRNFIDNALKYGGNRLTEVRIGYEESEQFHVF
                     SVSDDGRGVSREDTEKIFQVFQRSRGSSGVEG"
BASE COUNT         3572 a         5557 c         5677 g         3992 t
ORIGIN      
        1 atcgatcatc accaggagtt ccatgagatg atcgttcacg ggatgggccc ccgattgaac
       61 cacgaagatg tcggcacccc gcacgttttc ctggatctcg atgcggatct ccccgtcgct
      121 gaaggtgctc accagtgccc ttccaaggga cacttcgaga ttgctgcaga tctgctgcgc
      181 cagttccggg ttgctgttcc cggcaaaaag cttcaagccg taagtggcca ttgattccaa
      241 acctcgctca atatcctcgg gaaacgaaaa gcagaaaaag gcgccatttt catagtacag
      301 ccttccccaa atggcaacca ttttcctgac gggtgagtga cttgtccttc gaggctgatg
      361 cgatcccgga agacccttgc ggatccgctt gggaaaacaa ttcatccccc aggggtcgac
      421 ggtggtgacc gcttgggagc ccccggagac cgggacctgc ggtgggggga tgccggggga
      481 gggcgaacct acggtttcgg tcgtccgccc tcgaagaaat ggggagtctc gatcgggcct
      541 cgttcatccc cccgggcatc gaaaaggggg tgtacggcaa taccccgggc gggctttctc
      601 agaggttttc cgtttcctct tcggggaccg agtatgcctc atgtccgggc caatagatga
      661 accgatggca atggggacac tgcatgatgc tctcgtctcg ctggagctcg atgaacttct
      721 ggggggggat attcagacgg cacacctggc ataccccgtt ttcaaccgca gccacggcaa
      781 tccccgcctg cctcgtaatg aggttttccg aacgctttcg gaggtccggt ttcagttttt
      841 cccgcacctg ctcctgggcc gcttcgaacc gatcgaggcg ttccttgagc ttgtcgctct
      901 cggccttcag gacggctttg tcctgttcca gcgctttcct gcgctcctcc agctctttgg
      961 atcgggcttt gagctcgcgg cccagggttt caatggtttc caggaaaccc agaactgcgt
     1021 cctcgttgac gccgatctcc ttcttgattt cttcgatctc cttgaggatt gcctggtatt
     1081 ccttgttcgt tttgacctcg ttcatccggc gcttgctgcg ctggattctc gcctcgaggt
     1141 ccgcaatggt ctgttccgca gcacgatgca tcttcttggc gtgttcgtac tccgccttcc
     1201 tggaaaggta ttcggcttcg aagtgttcga attccttttc cagttcggaa accctggcgg
     1261 gggcatcctc acaactgcgg atcagcgccg ccttcttgtc ttccaggatc tggtattgaa
     1321 tcaagtatct aagctgttcc agcaatgcca acctccttca tgcaatagcg ggatattctc
     1381 cgaagagccc acatcctgtc cggcccttcg tgtcagccga agcctcggtg agtggtgaac
     1441 gggtcccgtt ccaagccgga ttccaggacc tccagctcca gctttcgtgt cttcgcctgg
     1501 gattccaggt atttggccaa aggctcgagg acgatccgct cggatgcgaa atggcccacg
     1561 tccaccagga ccaacccggc ctcgatcgcc ttctgggctt catggtactt gagatccccg
     1621 gtgatgtagc agtgggctcc ccgggagacc gcaaggtcca ccaggtttcc gccgcttccg
     1681 gtgcagaggg cgacgcggtg aatcttcccg gcgggggttc ccacgacgcg aaggtccgca
     1741 ctgccgaaga gtttgcgaag ccggtccaca agctcatcga ggctgaccgc acggcacagg
     1801 ttccccacct gccccatccc gaggtactga gcctctccct cccaccgggg attcacttgc
     1861 aaggggtcgg ggtctcgaag ttccaggagc tttgccaact gatcgttggt gccttcccgg
     1921 gaaacatcca ggttggtatg ggcgaccacc agggcgatct cattgcggat ggccctggcc
     1981 agaagatttc ccggatattg atccggacga acctgagtga ggggttggaa gaggaggggg
     2041 tggtgtgtga ccaggcactg gcactcgtgt tgttgggctt cgaaaaaggt tccggaactc
     2101 ggatccaggg cgacgagcac tcgccgcacc ggcatatggg gatctcccac ctggagcccg
     2161 caccggtccc aggattccgc gtagcgaaag ggtgcgtgtg cgtccaacca tgtcaggagg
     2221 tcttctactc gcaccatatt acacgagctc gccccaaaag aagaggggcg ctccttccag
     2281 aacacccctc gtgaaccgcc ccaaccgaac cgttggcgtt tccgtcgctt tcctctgccg
     2341 gatccctagg atccgcctcc ccggtatagg gaatagaaag aattttgttt ttgttggcgt
     2401 tctcatgacc ttcatcatgg ggttcgttag tgggcccacc tggcctcgaa ccagggacct
     2461 accggttatg agccggtggc tcttccagct gagctatggg cccctgccaa gaagtttccc
     2521 tattcagcga gtatataaag agagggctca aaccttgtca agcccaaacc acccctagct
     2581 ttcgataaag ctcttgagct ccttgcgcct gctgggatgg cgaagtttgc gaagggcttt
     2641 ggcctcgatc tgtcgaatgc gctcccgggt cacggcgaaa tcctgcccca cttcctcgag
     2701 ggtatggtcc gccttttcgc cgattccaaa acgcatccga aggaccttct cctcccgggg
     2761 agtcaaggtg gccagcgcct tccgggtctg ttcgctcagg ttcaggttga tgaccgcgtc
     2821 cacaggcgag gcaacccgct tgtcctcgat aaaatccccc aggtggctgt cttcttcctc
     2881 tccgatgggg gtctcgagac tgatgggctc cttggcgatc ttgaggacct tgcggacctt
     2941 ttccaccggg aattccatct tttcggcgat ttcttccggt gtgggttctc tgcccagttc
     3001 ctgtaccagg taacgggacg ttcggatgag cttgttgatg gtctcgatca tatggacggg
     3061 aatgcggatg gttcgggcct gatcggcgat ggcccgggtg atggcctgac ggatccacca
     3121 ggtggcatag gtgctgaact tgtagccccg ctgatattcg aatttgtcca cggccttcat
     3181 gaggccgatg tttccttcct gaatgagatc caggaactga aggccccggt tggtatattt
     3241 tttggcgatg ctcaccacca gcctcaggtt ggcttcgatc agctcgctct tggccaactt
     3301 ggccctgagc atcccttctt ccacccgccg gaggatttcc cggagggtct tggaatccat
     3361 tttggcttcc atctcgaggg taccgatgcg gtctttggct tccacggcac ggcacaccag
     3421 gtcgtggaac tcctgctgct gaattcccat ctgccgggcg taggactcca tttcgtcctg
     3481 gggccgttcg aaaaagggct tcaagtcctg gaagggcttt cctgaacgca gaagacactc
     3541 ctccagatcc cgctcggatt tttcgatcct tcgcaggtac aaacgcagct tgtcgatgat
     3601 gctgtcgata tgacgccttc ccagctggag tcccttgaga aggttcacga tttgctcttt
     3661 gttttgctgc accatctgtc tgagcccctg ggcatcctcc gctgtggtgt cttcccgcag
     3721 gagcgtcatg tgcatgatct tgttggcctc gtcgaggcgc tgtacgtttt ccagcatgcc
     3781 gatgatgcga ttcttctgga tttcttcttc ttcgggggtg atttcctcat ccatctcccg
     3841 aaccacgtcg ttgagccgaa ccacactgtt ttcgagccgt tccttcaagc tgaggatctc
     3901 gcgaatgccg atggaggatt ccatgatggc attgaagact tcccgttcgc ccatctctat
     3961 gcgcttggcg atttccactt ctccctcgcg ggtgagcaaa gaaacctgcc ccatctcccg
     4021 aagatacatc ttcacggggt ccgtgacccg gctggcgctg tcggcatcaa tctggagctc
     4081 gtcttcttcc tcctccatga ggcattccga gtccgcgccc gccgattccc gcaccacctg
     4141 cacctgctgt tcggagtcca caatctcgat gtccatttca tcgaagatca tcatcatgtc
     4201 gtccagtttt tccggggaca cgaggtcatc ggggagggct tcgttcactt cgtcataggt
     4261 gaggtaccct ttttcttttc ccacggtgat gagacgcttg aggtcgtcca tctccgtctt
     4321 ggcaagggcc ggatctcgtc gcggcttcat gtccttctca gtcatccgtt cgtctctccc
     4381 taaacgttgt cgggcgaatg cttcacgctg ttgctcaccg aaccgagctt ctgaatctgg
     4441 agcaggatcc ttctgacttg ggttgcgtct ccggccgttt cggcctgggc gagggcttct
     4501 ttgagccgtc gcctctcttc tttcaagctt ctgcgggtca gggcgtccag ccagtcccgc
     4561 aattgcacga tgggctcctc cagatcggca gattccatga caagacgggt caccagttgc
     4621 ctgaggtccg gatcggcaaa caggtcgtac agctccgagg acttgaggga ggccaggcga
     4681 ttcggcggaa ggtccaggag aacctgcacc gccttgcgga cccggggttc ccggaagcag
     4741 tccgcggccc cggaacttcg cacctgttcc aggacctcgg gatgatgaat caggagctgg
     4801 aggatcttct cctcccggct ctccggaagc gggcatgggg agacgggggc caccggggag
     4861 gcccaccggg cgggatgcga ccttccatgg cgaagctgcc gctcgatgac ggactcggga
     4921 agagccagtc cttccgagat cactctcaag tactcagacc tcaataccgg ttggtgcaga
     4981 gcctcgaaga tcggagccaa gtcggaaatg atttgtgcct tggtggacgt acggccgtcc
     5041 cagcccttca atgctttccg gatggcgcaa agccccaggt cctgccgggt attcatgagg
     5101 gcttccaggg aagaggcccc gtttgctcga aggaaatcgt ccgggtccat gccgtcggga
     5161 aattgcagga aactcaccgc aaggttgtct tgaaggcaaa ggggaagcgc cctgagcatg
     5221 gctcgttccc ccgcttcgtc tccgtcgtag gccaggatga cctcgtcggc cgcccgggcc
     5281 aggagacgca cttgcggggg ggtcagggct gtgcccaggg ttgccgccac ccgctcgaat
     5341 ccgtgggcat ggaatgccag caagtccatg tagccttcca ccaggacgat ctttcgctcc
     5401 cggcggcacg cttcccgggc cctggccatc tggtagagca ttctgccctt ctggtagatg
     5461 ggcgtttccg ggctgttgag atacttgggc tcatccggtg cgctcccacc gaggattctt
     5521 cctccgaagg caaccacccg gtcccgttcg tctcgaatgg gaaagatgat tcggttgcga
     5581 aaacggtcgt agagctgatc ggggttcttg gagctcttgg acaggaggcc cgccgtaacg
     5641 gcgatttccg ggttcacacc cgacttccgg aggtatcggg ccagggaatc ccactgggga
     5701 ggcgcatatc ccagtttttc ccggcgaatg agctcctcgg gaactgcccg gttcttgagg
     5761 taatcccgtg cgtccctacc cgtccgggcg tggacgaggt tctgatggaa gtaatcgccc
     5821 gccagttcca tgaccgcgta gagccggtct ctttcttttc gcacggaagc cagggcttcg
     5881 ggtgagaggt tcgaggacgg atctttcctg ggtaggttta tatggtatcg gtcggcaagg
     5941 tatcggatgg catccccgaa aaccaggttc tgctgcctca tgataaagct caaaacatcg
     6001 cctccgcttc cgcaaccgaa gcagtaatac aactggtttt cggggtccac ttgaaaagac
     6061 ggggtctttt ccgggtggaa gggacaaatg cccgtgtact tctttcccgt gcgacgaagg
     6121 ggcaccacct gaccgatcac atccacgata tcgacggctt gtttcaccag ggcagcggta
     6181 tccgaggcgc tcacggaaat aagcctgtcc ctcattttct ggaaggtccc ctcgtaatgc
     6241 gatgcaacct taaattttta tccagttgga aattccaaat caagggtctt gtcgaaaaaa
     6301 cctcggagcc tcagccaccc agtttcttgc gagccagttc gtttacttcc ttgccgtcgg
     6361 cccgcccggc cacacgggac atgaggacct tcatgacctt tcccatgtcc ttcagggact
     6421 gggccccggt ctcggcaacg gcctccagga ccattttctc caactccccc ggtgagaggg
     6481 cctccgggag aaagccccgg agaagaagga tttcttcttc ctcatgtcgg gccaggtcct
     6541 gtcggttccc tttgaggtat tgttcggcag cttcccgtct ttgcttgatc agggtggaga
     6601 tgatttgttg gacttctgcg tcgttgggga gcctcttgag ctccttctcc ttgagtttca
     6661 cggccgtcag aagcagtcgc agggcgtttt tttgatcttc gtttttcttg cgaacggcct
     6721 ccttgagtgc ctgttcgatc cgggacatca aatccatcat tggcctcctt tgggcttctc
     6781 gcccacggat gcggcggcgc gggattggag gaattccagg gcatcccgca ggtccaaagt
     6841 cccgctatag agggctttgc ccgtaatgac cccttcgaga cccaagggaa tcaggggcag
     6901 cagcgcctcg atatccccca gggtcgccac gcccccggat gcgatcaccg ggagccgggt
     6961 ggcttccatg agacgccggg tggcttccac gttgactccg gacctcattc cgtcccgatg
     7021 gatgtccgtg taaatgacgg cgctcagggg aaggacgtcc agggtacgaa ccagttggac
     7081 ggcgtccgtc ctcgaggttt ccttccatcc ctcgacggcc accatgccgt cccgggcgtc
     7141 gattcccacg gcgatgcgcc ggggaaaaag ccgggaagcc tcttccaaca aggccggctc
     7201 ccgaagggcc accgtaccca ggatgaccct gcgaaccccc agggacagat actcctccat
     7261 ggtctccagg gtgcgaatcc ctcccccgac ctggacgggg atggatacgg cccgcaggat
     7321 ggaagccacg acctggaaat tcttggggac cctcgagaag gccccgtcca ggtccacgac
     7381 gtgaagccac cgggcgccct ggtcctccca tcttcgagcc gtttccaccg ggtccgttcc
     7441 atagaccgta gcacgttccg ggtccccttg catcaagcgc acacagagcc cgtccttcaa
     7501 atcgatggcg ggaaaaatga tcatcgtacg gtccccccag gatgttggag agggtccctc
     7561 ggttcatggg aatcgagggt tttcattcct ttgtcagcag agccttcagg taatgcggag
     7621 gcttgagcac cttgcgatcc cggttcacgc acacatgcac cgtgtacccg gaggccaggg
     7681 tgaggggatc gcgcctgatt tcgtactcga acctcagccg gatgggtccc acgaaccgaa
     7741 agcgggtgga aatgatgagg atgtcgtcgt agaaggcggg cttgtgatag gagcaatggg
     7801 cttccaccac ggggagaaag atccccccgc cttccagttc ccgataggtc attccccggc
     7861 tgcgaaacca ttcggcccgg ccgacctcga accatttcag gtaattgccg tagtaggcct
     7921 ggcccatggc atcggtgtcc ccgtacaaga cccgtatggt tgcctgggcg gcgggggatg
     7981 cctcggagat ctgcggatgc acttccctgc tttccgaagc ccggatgtcg ggatcgactt
     8041 tgccggccat gtccggtttg ctctgttctc tcatgaaggt gggtttcaaa aggggtcctt
     8101 acggggccgg tttccccggc ggaaagaagc ctcgccgatt cttggcctgc tccggggggg
     8161 cgctcgaggt cggctgcatg aggagcccaa agagctcctc gccgctggcc actttgatcc
     8221 ccgtccgagc ggcggatttc tcacagaagg ttgcaggagg cccggtactt tccttcccgt
     8281 cgttcaaatg ggatacgaga agaaagggca ccgggtccga aacatgggtc cgcttgtgca
     8341 ccggggtgag atgatccgtc accaccagca gccggacccg cgggaatttc tccatccctc
     8401 ggacgacggg tcccaccacc ttttcgtcga agtcttcgat ggcttggatc ttcttggcta
     8461 cactcccctc gtggccggct tcatcggggg cctcgatatg gacgtagacc aggtgtccgg
     8521 cctccatggc ctcgagggcg gcctgcacct ttccccggta attggtgtcc agatatcccg
     8581 tggctcccgg caccgggacg ggatccaggc ctgcgtagac ccccaggccc tttagcaggt
     8641 ccacggcgga gatcatcgtc ccgacaatgc cgaatttctc tcgaagggtc ggcatggacg
     8701 gggctttccc ctgtccccac agccagacgc cgttggcggt cctcttcccc gcctcctgcc
     8761 gtcgttgatt cacggggtga ttggccagaa cctgggcggc ccgttggatg aaagaatcca
     8821 ggacgggttc tctccggtac acctccccat agggcccgac gggctctccc ggaatatcgt
     8881 ggggaggggt ggtccggaga tccgggtttc ctcccgccca gaccagaaga tgccgatagc
     8941 tgaccccggg atagagccgg agcggaccaa ggacggcctc ttcttggagg gcccggatca
     9001 attcacgggc ttcgggagtg gagatgtgtc ccgcagaata gtcccccatc cgcatcccgg
     9061 aaggagcttc ttcgaggttt accaagttgc agcgaaaggc cacctcgtcg ggacccaggc
     9121 gaatgcccat gctggccgcc tcgagggggg ccctccccgt atggtaccga aggggatcgt
     9181 agcccaggag gctcatattg gcgatgtcgc tgcccggttc catggatgcg ggaatggtcc
     9241 gggccaatcc catgagtccc cgggccgcaa gtccatccat ggtgggagtg tgcgccgctt
     9301 ccaggggggt tttcccccgg agttcctcga gggggtagtc ccccatcccg tcccccacca
     9361 ggatgatggt tctccagtct tccaacgcta ccttcatgag gtctctcaag ccatctggtc
     9421 ggtttcttgg ttttcgatgc gaatgaattg ggtgggggcg tggaccacct ccagccggtc
     9481 gatctcttcc agggcgttgc gcacatcggc ttcccgggct tcgtgggtga gcatcacgat
     9541 ggggaccgtc tcctccgcct ggcggccttt ctggatcacc gcggcgatgc tgatgtcgtg
     9601 ttgtcccagg attcccgaaa tcttggagag cactccagga cggtccaccg cggcaaatcg
     9661 aaaataatag caggtgacca cgtccgccat gggtttgacg ggcaccgggc ttattctgtc
     9721 ttccgggaag gccaggggag gaatccgggt ggggaccccc agttggatat cccgggccag
     9781 gtccatgagg tccgcggcga cggcgcttcc cgtcggcatc atgcccgcac ccaagccgta
     9841 cagcatgatg tttcccaccg cgttgccgtg aatgtgaacg gcgttgtagg cgcccttgac
     9901 gctggccagc acgtgctggt tggggatgag agtcggatgg accctcattt cgatcccatc
     9961 gtcggtgttt cgtgcgatgg caagcagttt gagctggtag ccgaattctt ccccaaaccg
    10021 aatgtccacc ggatcgatct tggaaatccc ctcgatatag acctggtcaa actggatcgg
    10081 agtcccaaag gcgatggccc ccgcgatgct cagtttgtgg gccgtgtccg ttccctcgat
    10141 gtccaggctg ggatccgctt cggcgtaccc ccgagcttgg gcttctttca gggcgtcctg
    10201 aaaggagagc ccttcgtccg tcatgcgggt gaggatgtag ttggccgtgc cgttcaggat
    10261 tccgaagatg gtttggatcc ggttggctgc gaggccctcc cgcaaggact tgatgaaagg
    10321 gatccccccc gcaacggagg cttcgaactg gatggatcga cccatttccc gggccagggc
    10381 gaaaagctcg tttcctgcgt gagccagtag ggccttgttg gcggtgacga cgtgcttcct
    10441 gttttgtagg gcccgggaga tgatctgctt tgccgccccg attcccccca tcaattcgac
    10501 cacgacgtcg atgtcgggat cctcgagaat atcgtccgca cgagtggtca gaattttcgg
    10561 gtccgcctgg acgggcctgg gacgattcaa gtccaggtcc gccacccggc gcagctcgag
    10621 ccgggttccc agccgatttt ccatgagtgc ggcgttttcc cggagaatcc gaatgagacc
    10681 gcagccgacg gtcccccatc caatcaggcc gattccaatg gaacgcatct tccccccaat
    10741 ccgcatgaac gaagtcttat gcccatcccc gtcgccgccc cctcgtttcc ccggcaagac
    10801 ggagacgaat cggcttcccg gaaaaggggg atcgtggaag gtccccggcg accggtaatc
    10861 tcaaacagac aatgaacgac tacttttctt gaagcgcctt gcgcattccc cggatggcct
    10921 ggcgggttcg tttttcattc tccaccaggg caaagcgcac gtattcgtcg ccgtattccc
    10981 cgaatcccag cccgggggag accgccacct tggcttcctc gatgagatac ttggagaatc
    11041 ccaccgaacc catgtctcga aaccgctccg gaatgcgtgc ccaaacgaac atggttcccc
    11101 ggggcttttc gatgtgccac ccgattcggt tgaggccgtc caccaggacg tcgcgccgaa
    11161 cccggtacac ttccgaaatc tcccgcacgc aggactgatt tccgttcagg gcgatgatgg
    11221 cagcgatctg gatgggttgg aaaaccccgt agtccaggta gctcttgatt cgagtcaggg
    11281 cggaaatcat ttccgagttg cccacgcaga accccacacg ccacccggcc atgctgaagc
    11341 tcttggacat ggaaaagaac tcgaccccca cctccttggc tccggggacc tgcagaaagc
    11401 tgggggcccg gtatccgtcg aaggtgaggt ccgcatacgc caggtcatgg atgaccatga
    11461 ttcggttttc cttggcgaaa tccacgatct ttcggaagaa ttccaagtcc accacggccg
    11521 tggtaggatt gtggggaaag gagatgatca ggagccgggg ccggggccag gtctgtttgg
    11581 ttgccttctc caggtcttcc aggaaattgc tgtccggact gatgggaatg gatcgcacgt
    11641 cccccccggc aatgatggcc gaatatggat ggatggggta ggtggggttg ggcgaaaaca
    11701 ccacgtcccc cggactgatg agcacgagca cgagatggga aagcccttcc ttggcgccga
    11761 tggtcaccac cgcttccgac tcgggatcga tgtccacgtc gtaacgttct ttgtagcgcc
    11821 gggcgatggc ttcccgaagc ttggtaatcc cgcgggaagc cgaataccgg tgattgtgag
    11881 gcttcgccac ggcttccacc agcttgtcga tgatgtgctt cggggttgcc aggtccggat
    11941 ttcccatccc caggtcgatg atgtcctccc cggcccttcg acgctccatt ttcaaggcgt
    12001 tcacctgggc gaagacatag ggcggcagac gatggatccg gcttcgttcg gcaatcttga
    12061 aactcatggc tgaccctcct gtaaattaaa tcatcgaacg atatcgtaga gatgcaggga
    12121 tgtcaaaatc ttttctgagc aaggtcccgg actccggccc cttcccggcc ctcgatgaaa
    12181 gcgccggggg ggacccttcc ggccgggttc ccctggaggt tccgtgggcg aaatgccttg
    12241 aattcccggc aaaaattctg ttaattttcc aaacgattcg agtcgcgggc aaacggcggt
    12301 tcctcatttt tgaaggcaca aacctatgaa gcaagtcatc ttggggactg cagggcacat
    12361 cgatcacgga aagacctccc tgattcgcgc cttgacgggc atcgatacgg accgtctcaa
    12421 ggaagaaaag ctcaggggca ttaccatcga actgggtttt gcctacctgg atctgcccgg
    12481 cggagaacgc atggggatcg tggatgtgcc ggggcatgag cgcttcgtcc ggcatatggt
    12541 ggcgggagcc gccggcatcg acctggtggc cctggtcatt gcggccgacg aaggggtcat
    12601 gcctcaaacc cgggaacaca tggagatctg cgatcttctc cgggtaaaga aaggcctggt
    12661 ggtcctcact aagaccgacc tggtggaaga tcccgagtgg atcgaaatgg tgaaagagga
    12721 cgtggcggac ttccttaaag ggacctttct ggacggagct cccgtcatcc ccgtgtccgc
    12781 agtccggggg acggggatgg acgagctcaa ggaacagctc gccgtgttgt accgggaggt
    12841 ggaaccgcgc agccccgagg gtccctttcg gctttccgtg gaccgggtgt tcaccatgag
    12901 gggttttggg acggtggtca cggggaccag cctttcggga agattgcggg tgggagaccc
    12961 ggtcaccgtc tatccgtccc tgcacaagac caaggtccgg ggcatccagg tccacggcga
    13021 agacgtagag gaggtgcttc ccggccaacg gaccgccatc aacctccaga gcatcgaaag
    13081 ggggctgatc cggcgagggg aagtcatcgc cagtccggga gccctggtgg ccactcacat
    13141 ggtggacgtt cacctggacc tcctctcgag ctcgccccgc cctttgaaaa atcgcgcaaa
    13201 agtccgcttc cacacaggga cggccgagca ccttgccgtt ctggttctcc tcgagcagga
    13261 ggaactggcg ccgggcgggg aaaccttcgc ccagattcgc ttggacacac ccattgccgc
    13321 cctgagggga gatcggtttg tgttgagatc ctactcgccc gttcgaacca tcggaggcgg
    13381 aacgatcctt catccttttc ccggcaagca caagaggcag gaaaagcgca aggctgccgt
    13441 ggcgctcaag gagctgcttc tggccaacga acccgacgcc gccctctggc atgtcctcca
    13501 tagcggcccg ggggggatca ccgaggagga attgcgggtc cgggccaaca tccctcccaa
    13561 gtcgctggac aaaattctgc agcagtttac gagtcaaaag cgggtcatcg tggtggacaa
    13621 ggacaaccgt agactgattc accccgacgt gctgcaggag ctcaaaggga cggtccttga
    13681 ggccctcacc gagttccaca cccagttccc cctcaagacg gggatggcca aggaggagtt
    13741 gtccgcacgc ctcccccggt ccgtggacac gaggctctac aacttcatcc tgcggcaact
    13801 tgcggagcag gacctcgtcg tgcaggagat ggaatgggtg cgcctgagtt cccacaagac
    13861 ggatctgagc aaggaagagg aaagtatccg agagaagatt gaaaaagcct acaaggatgc
    13921 ggggctgcaa ccgcctttct tcaaggaagt ggcggggacc ttgccgggca attcccgccg
    13981 gcaccaggac gtcctcgagt ggatgtttgc ccgggggacc ctggtcaaaa ccaaggaaga
    14041 tctctatttt cacgcatcgg ccctgaagga gcttcaggat cggctggtat cgtttctgcg
    14101 ggagcacggg gagatttcaa cgccccagtt caaggacatg acccaggcca gccgcaagta
    14161 cacgattcca ctgctcgagt atttcgactc ccagaagatc accatccgga tcggcgacgt
    14221 ccggaggctg agggaaagcc gggccggggg ctgagaattc aacctgaaag gggaaaagcg
    14281 aaggacgcat tcatgctcaa acacgttgtc ttcatgaagt tcaagccggg agtgacggaa
    14341 gaggaaattg gggacctggc ggggagcctg ggaaaactgc ccggcgtcgt tccggaaata
    14401 cagggcttcg agttcggccg ggacctcgtt cgctcggaac ggtcctacga tttcgccctg
    14461 gtttccacct ttgcggacct ggatgccatg aaccgctacc aggttcatcc cgaacaccaa
    14521 agggttgtgg cgaaggttcg acaactgagt cagagcgttt tggccgtgga ttttgagacc
    14581 cggtagccgg tcgcccggca tcccccggaa gacggagaag cggaccccga agggcgatag
    14641 cccgaatccc cgggataggt tttcatccct gcgcccgccc cggacccgat cccgggctct
    14701 ccgatgcgag gggaaattga ggcggatagc ccgtccgacg gtctcttttc gcctccttcg
    14761 ggactcgatc ttctcccgga cctgattcga ggagttcgga acccgatccc gagaccggga
    14821 aacgggggga agggctgctt gagaaaaccg aacgaaggta ggaagccgat attccaaacc
    14881 cgtgtttggg tgtgattctt ggaggtcctt atggtaggca acccgagaag aaacgaggat
    14941 accaaggccg ggctcgacga ggcggccgga gaagtgtctt cgcgacatgg attcccagcg
    15001 gaccaacgat cgaggtgccg tcccgaaaaa cgacggcagg agaaagggac ccggttgcac
    15061 cgggacaaac ggatggtttg tatggttgtg ggtctctttc tgctgacggc tgttccgggg
    15121 tttgtcccgt ccgcccgggc ggattcctct tcgcccttcg agctcgtccg gtccggttcc
    15181 gatcgaggat tgcggatcat caagagctcc ttgtttgaag gaggtcccgg tctccaacag
    15241 cgcagggacg aaatcctgag aattgtcgag gaatacttcg actttgacga aatcgccaaa
    15301 cgggccctgg gcagaccgtg gaaggacctc gctccggaga aacggcagga attcgtccgg
    15361 cttttcaaag agctcctctt cgacacgtat gtaagccgca ttgaagccac agccaggccg
    15421 gacaccggca ttcgttacga ccgacaatcc gtcgaaggcc ggtatgcggt ggtgaagaca
    15481 tgggtcacca atgccggccg gccggacttc cagatcgact accggcttct tctctcgggc
    15541 gggcagtgga aggtatacga tgtcgtcatc gagggaatca gcctggtcgg caactaccgg
    15601 gagcagttcg cctccatcct caccaacgaa tcctttgatg ccctcttgaa gcgcctccag
    15661 gaaaaggtcc ggacacgatc cccgctgcaa tgatcgggaa agggggcatt cgtttcgcca
    15721 aacgcccggt cccgcgaggg gctcatggcc accggacacg gcaacgtcaa ggttgtccag
    15781 gttcaagacc ggagccatcg gagaagtaga ttgccccgaa gtcgagcagg gtgtaccata
    15841 tcgggttgtc ggcctgttga ccggaagtgg ggacgacatc agggccccaa tatcgatcgg
    15901 ggacgacccc gcgattctga aaggaacacg gatggcttgg gtgattctta ttgttgcggg
    15961 gctgttcgag gtcggttggg caatcggaat gaagtacacg gagggcttta cgcgcctgtg
    16021 gcctacgatg tggaccgtgc cggccatggt cctcagtctt tggctgctcg gcattgcgat
    16081 gaagtcgctt ccggttagta ccgcatacag tgtgtgggtt ggggtaggat ccgtcggcac
    16141 ggtgatcctc ggaaacgtgc tcctcggcga accggtgaac gcggctcggc tcatcagcgt
    16201 tgggctcatc ctcgccggca tccttggtct caagcttgcc acaccggcgt gacaaccggt
    16261 gccccggacg ccgggcaagg ggcgggcccc cgaacgggag cggtatgcac cggagcggcg
    16321 gcaatcgggt ggagccgcac ttcaccggaa cggaccttcc cgggttttgt tgatttggta
    16381 tgacaatcgg cgcaaaggtt tttctctcgc agcttccagc cgatggtttg gggagctgag
    16441 cgtgaattcg ggtctttggg gctcaacatg cccggctttg gcactcaagg ccaaccggtg
    16501 aaagaggaac ctgccggcgg aaaccgggac ccggagacga gggcgcgggg actccgcaac
    16561 agacaaaaat ccatcttggc gcaccgcaca cggcagattt gggatgccgg gcccgtgggt
    16621 ccggggtccg gtgccgtata aggaatcaat ggcgcaatga gaccccggat catgccccgg
    16681 ctgttttcga gtttgcgatt tcagctcatt gccattgtgc tgctgtcggt gctgcctgcc
    16741 attgcactga tcctctattc cgggttcgag gagcgaagaa acgccgctct acatgcccgg
    16801 acgaatacca ggaggctggc cgaactggcc tccattcagc aagcacgctt cattgacgaa
    16861 gcctaccgcc tgctcctgac cctctcggaa agcccgcatt tgtactcgtc ggatgcggaa
    16921 cagtgttccg catacctcct ggatgtaatc tcgtggcatc ctcactacct caatatcggc
    16981 gtcctgggaa cggacggcag ggtcctgtgc agtgcgcttc ccgtggaaag ccgtatgccc
    17041 ctgcccaacg aggacttttt tcgccacgcc cttgagaatg aaggccttgt catcgggagt
    17101 gcccattctc gggacgattc ggggaaggcc tcgttcgaac tgggataccc ggtccatgat
    17161 tcggcatacg gcgtgagagg cgtggccttt gccgcggtag acctggcctg gttgagtgat
    17221 cttgcggcgt atctgggttt tcccgaaggg gcaacccttg ccttgacgga tcgggatggt
    17281 accgtcatcg ttcgataccc ggatcccgag cgatgggtcg gcaaagtcgt ttcgaacaaa
    17341 gccatcgtca agacagccct ggaacaaggc aaaggcacga tggaagcggt tggggtggac
    17401 ggtgtgaatc gcctttacgc cttcaacgtt ttgggcgact ttgcgcagcc cgtcatcctg
    17461 tacacgggca ttccgacgag cacggttttt gccgaggcca accgggcctt gacccgcaac
    17521 ctcatcacct tgggcatcgt tacgatcctg gcgctaatcg tggcccatgt gctgggacat
    17581 ttggtggtca tgcgccgggt gaacgccctg gtccgggcca ccgatcgaat cgggcggggg
    17641 gaactggggg cccgaaccgg catgtcatcg ggaaacagcg aactggaaca gctggggacc
    17701 gctctggacg ccatggcgga atccttggag agaaagggcc tcgagcggga cgagatggag
    17761 gctgcccttc gcaggtccga agagcggtat cgcgccttgg tggaacagat ccccgtcgtc
    17821 acatacacgg cggccctgga tgaagcgcgc accatccttt tcatcagccc ccaggtggaa
    17881 tgtctcctcg ggttcgatga gggtgatttc tacgaagatc gggatacctg gcgaaaaagg
    17941 ctccacccgg acgaccggga aagggtcctg cgggagtcgt cgcgttgtag aaaggagaac
    18001 gatcttttca tctgcgaata ccgcatgctg acttaccacg gcctggtgaa gtggatccgg
    18061 gacgaggccg ccgtggtgct ggacaaggcg ggggagcccc tgttcatcca gggggtcatg
    18121 acggacatca ccgagcagaa aagatccgaa gaggaactgg tggaggcgca caaagagctc
    18181 gagattcggg tggcccaaag gaccgaagcc cttgcccggg caaacgagga gctgcgccag
    18241 agcgccgaga cgctgaagtc ctttgcggcc tctgtggtgc atgatctgag gagtccggcc
    18301 attggcgcct acggactcac aaaactcctc tggaagcaat acggggacgt cctcgatgaa
    18361 aaaggcacgc actactgcga ccagatcatg aaggcttccg agcacgtggt cgagcttgcc
    18421 gaaaagatca atgtctacat ggccacaaag gagaccccgt tgtccatcga gacggtttcc
    18481 ctgggggagc tcctgggtac tctcagggac gagttttcct cgcggttcag cgctcgcgcc
    18541 atccgctggg tccagcccga aagggacgtc gaggtaaggg ccgacaggct ctccctcttg
    18601 aggatgttca ggaatttcat cgacaatgcc ctcaagtacg gcggcaaccg tttgaccgaa
    18661 gtcaggatcg gatatgagga atcggagcag ttccatgtct tttcggtgag tgacgacggc
    18721 cgcggcgtca gccgggagga taccgagaag atttttcagg tgtttcagag aagcaggggt
    18781 tcgagcgggg tcgagggg
//
LOCUS       DAOOVC010000066        18656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2029_length_18656_cov_6.584001, whole genome shotgun sequence.
ACCESSION   DAOOVC010000066 DAOOVC010000000
VERSION     DAOOVC010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2029_length_18656_cov_6.584001"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PLM79_09930"
     CDS             complement(<1..490)
                     /locus_tag="PLM79_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86670.1"
                     /translation="MAAENPDVMQIMELLLTAEIFNQSPSLTENDLPPSILAHYLDFD
                     LQGVERPIVVTSQDVKKIYGIDNAPDLLRLLPFIHVEDSGSRIGLLDFDLAAGWFGGR
                     DTLPAMQRNPVLAHYYQKFDPNVSYIEARVANKPVRRETEGAEAKIEDLWDPAFKGKV
                     SLL"
     gene            1007..3202
                     /locus_tag="PLM79_09935"
     CDS             1007..3202
                     /locus_tag="PLM79_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOV86671.1"
                     /translation="MNQDPPGKQVERLLEALVGGGVSLDRDRTDLSRLVRDARSFQCR
                     DIRVVAFGGGTGLSTVVGGNSQLEDWPENPFIGLKQEFPFLDVVVCTTDDGGSTGLLL
                     QELPMIGVGDLRKLLLSMILEDRLRETYGLDPIETRQLVRLIHGLFNHRFPPNHNPFK
                     CLSNPLLVVPRSWRKSCPESLARLLATLGQYVAPGGDGPTLKPGGHCLGNLILTAAIL
                     REASIRGVDPPDVESIEKGLDTLAEVLGVVPGRLHPATSTPGQLVLRYSNGVAVRGQR
                     KSAVCRRDFPIDCVAVEFFGNPAVKQDLCRTIREADLILFAPGSLYTSILPILQLTDV
                     VTAIKENRKALKILGANFWIQEGETDISLRGRNRGFTVSELVEAYDQNVPGGSAGLFD
                     VVLSANLEHVPGNVLSNYALEGKSPIYLDRSRLAGFGVLPVEATIYSRRRLRVAHVIH
                     HDPEKFALAVRGLVFAHDRFNLRCESLRPHASRPLRQCAPVGISALCDYRKAMSRELD
                     PKRFLPRQLRRVFDEILWENRDIRPGHLRNFRGARIIPSARWHRSNEWDNVLGYYDPE
                     DKMIYLHEQLRNDPERLKGNLLVALGESLLGRYLEGRRWIDWESRGEWGARCYEIRLR
                     PEEERECFLGDAQLKEYLRLSRMVPNPEDPRTYRITLNGNEGFTPPGLLFGLLYAWYL
                     NNDYGEILEYEMSLLRRPPQKLIPCQLQERDRKQALIALFRKEVFEHAE"
     gene            3407..4651
                     /locus_tag="PLM79_09940"
     CDS             3407..4651
                     /locus_tag="PLM79_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HOV86672.1"
                     /translation="MELRPRSHQKIVELIQKGVEIPNPLTLDIGDEVLPENISGNGVK
                     IYPGCRIYGKETVIAGGAKIGAEGPVTIDNCQVGPMVELKGGYFKGSVFLAKANMGMG
                     AHVRDACILEEEANGAHCVGLKQTILFPFVTLGSLINFCDCFMAGGRSRKDHSEVGSS
                     YIHFNYTPDGDKSTPSLLGDVPRGVMLNQPPIFLGGQGGLVGPLRLGFGNVSVAGTIL
                     RHDTPEDRKKIIGLTYPPTVVDFVPYRYPNLSRILKNNLIYLANLVALECWYRNVRKP
                     FFSTMEYGELFYRGVLDKLAAAKKERTGRLKAMVQKVPPSHDQCRCGKESEKGCELHA
                     NIDAVCELFGQSLSDPREAQLAEGFLNAFEKFRGANGRDYIQVIQAMPESLSAEGVRW
                     LQYVVDALCRRAGALLSSFGLF"
     gene            4694..6043
                     /gene="glmM"
                     /locus_tag="PLM79_09945"
     CDS             4694..6043
                     /gene="glmM"
                     /locus_tag="PLM79_09945"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOV86673.1"
                     /translation="MGTLFGTDGIRGVANQYPMDAAMAFAVGQAVTHVLKRESHRTRI
                     ILGKDTRISGYMLESALLAGITSMGGDPYLVGVLPTPGIAFITESMRADAGIVISASH
                     NPYQDNGIKIFGGNGYKLSDEQEEVIEKLILEGDLPKKVPPPHQMGQAYRINDVHGRY
                     IVFLKNTFPRALSMEGMKIVIDAANGATYRVAPETFTELGAKVDIIHNTPNGININDR
                     CGSQHTQDLRTRVVEKGAAIGLAFDGDGDRLIAVDEKGRQITGDQILIICSKMLKEQG
                     RLKNNLLVSTVMSNLGLTVACRKYGFRQHASKVGDRYVLEDMQQLGGVLGGEESGHVI
                     FLDHHTTGDGILTALQLIAAMLSSGKPLSELAGLMDIFPQKLINVNVSRKPELSTLPE
                     VVAAIKDVESALGDQGRVLVRYSGTQNMCRVMVEGPTDEITAKYCQQIVDAVKAAIG"
     gene            6123..7145
                     /locus_tag="PLM79_09950"
     CDS             6123..7145
                     /locus_tag="PLM79_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOV86674.1"
                     /translation="MAFTIYVTSDWDQMSQVAAELIESDIRQKQTAKREYVLGLATGN
                     SPTGIYKHLAKAFNAGRISPRTIRTFNLDEYVGLPGENPQQRALHCESYSYFMISEFF
                     GLLREKFAETNVPWGTLVDQDHLVRALEDHKDCYEFQGTDAGKAIVIGDQAQGYLKTI
                     KEEILDAYWKKIDACGGIDLQVIGVGGRGHVAFHESGIPFENNKVILVKLDENTVANA
                     VEDGHFASREESPRYAVSMGAEQVYRAKTVVLVANGSRKTGPVTEAVLGEVTPDVPIS
                     YGQKYAARGGNMVYVLDEVAAAEVLKRSAEVKAKGYRIVDKRGEPFAKVESLTFIRDV
                     RTGRLG"
     gene            7617..8534
                     /locus_tag="PLM79_09955"
     CDS             7617..8534
                     /locus_tag="PLM79_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86675.1"
                     /translation="MNDGLEQKIVRLLEEKGPLTGSEILERIRERNFLLWRTCYLSSK
                     LDIRTLGTRYLRLDQKVDGYARLSPSILREFMTYTLVGVSGAREGLDRRADRILRHIR
                     EVSQSKFALAHSIATRLQNDLNDRWPREGRLCFILAGDIVYDMAHDVPRPEASTGELV
                     NGSDIDLVVVADDTVPDDFLEGLDGAIYREKYRTLISPGVREELDYVVKKMERVREQL
                     GFDTFKRMVACKILREGALLLGSESLFREIKTLLVRSGVVEKLEEMEKQAGALRRRAE
                     EHLLQTDPSRIVEEDLNHFHTSEETEEFE"
     gene            complement(8615..10627)
                     /locus_tag="PLM79_09960"
     CDS             complement(8615..10627)
                     /locus_tag="PLM79_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOV86676.1"
                     /translation="MGREIDYKPRLNFRCCRLQPFSIMDRVVNMSEPQPSPGDPGSPH
                     KSPPLFSRTTLSLALGSLGVVYGDIGTSPLYSIRECFHGTHAVALTQGNVLGVLSLVF
                     WSMTMVICVKYIVFVMRADNRGMGGIFALLALIPSAGRRMSPELHSAIVLFGILGASL
                     LYGDAVITPAISVLSAIEGLEVATEAARPMVLPLTCGVLLLLFLVQHRGTADIGSVFG
                     PIMIVWFVTIAVLGGKEILQRPDILYAVNPLHAYEFFAVNRLHGIVVLGSVVLCITGG
                     EALYADMGHFGRNPIRLSWLGLAFPSLLLNYFGQGALLLEDPNMAFNPFYGLVPRYLL
                     YPMVFLATLATIIASQAMISGIFSVTQQAVQLGLCPRLQIVHTSRETQGQIYIPEVNY
                     LMMIACIGLVLVFKKSSGLAGAYGIAVTADMAFTSILYFFVITRTWGWSLAKALPLLA
                     LFLTFDLSYFSANLLKLFDGGWVTLTIAAVIATAMVTWKDGRAELARKMLSSRLPVDL
                     FLEDVERHDPQRVRGTAVFLSVSPVGIPSSLLLHYKHNQMLHEKVVLLSIRSVDVPVL
                     PDEEKVKIEDLGQGFFRIIAFYGFAEKPDVPRIMQLASLQGLSTQPATTTYYLSRESL
                     LTGGDSKMQRWRKALFAYMSRNARTATAYFSVPHDRVIELGLQVEL"
     gene            10885..11811
                     /locus_tag="PLM79_09965"
     CDS             10885..11811
                     /locus_tag="PLM79_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86677.1"
                     /translation="MMEKAVPGRMNVQLALLSGLILLVVLVVAGLWYKDKEHDLESVF
                     HSTLQKQEIVSRMRISLLKSAELEKAAVMADTDPLSRSLADQSLQAAEETDRQRRELN
                     RLIEHDSLAEEVALLKEFDHCWSQSRKIDAVLLDFAVENTNLKAARLSFTTAGKTLEL
                     FQQRLTDFLGSMASNERECRGGKAVFAALAAGFKIQYLHAPHIAAANDEQMDAIEKEI
                     EASREGVKSSLKELEGLASEENRELLRPAASAFAEFEAVCAEVVRLSRQNSNIRSLEL
                     SLGRKRKITSECDEILEGLQAVIRKRTPDATR"
     gene            12171..13424
                     /locus_tag="PLM79_09970"
     CDS             12171..13424
                     /locus_tag="PLM79_09970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOV86678.1"
                     /translation="MRKNSPSSVLVLGLLALAVLLNCSGCATSPSTKTGTGTMCPLTP
                     PVEPTSVGPSKPEAVVFMVPYHAPPKKIDLCGEPVPTERQEVLERLDKEFTLIVYNHA
                     QVYLWLKRMERYFPWIDERLSAQGLPSDLKYVAIAESDLLPNACSPKGAAGPWQFMPA
                     TGRNYGLDQRGDCDERYDFEMATESAFRYLRNLNNRFKSWTLAIAAYNCGEGRIQEQM
                     RTQGTRDFYQMKLPQETERYVLRILAIKAVLGSPEQYGYHLPRGEGYPPFKVDRVKAT
                     FSRTVSIQSVAAAAGTNYREFKRLNPVFRGDDISPGTYELKLPQGSGAIFQKNHAAAA
                     TSTADVSPASPSKTGVSSEDRKAADARGRSDSAKGRSARVHVVKKGETLSGIARSHQV
                     SVHELQRANKLKGSVVTPGQKLRIP"
     gene            complement(13453..14358)
                     /locus_tag="PLM79_09975"
     CDS             complement(13453..14358)
                     /locus_tag="PLM79_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOV86679.1"
                     /translation="MPDPPHVRNLEDRGAMKDRAPDPLPDFVPPFTREGLEDLYEKWN
                     RREFVHPDPLEFLYGYSDPGDREIVGILASCLAYGRVSQVLSSVSRVLERLGPSPRTF
                     ILRHDRREFRKILEGFKHRFTTGDHLSELLGGIREAVIRYGSLESFFVRAWEEADGSL
                     CAALSHFAGRLGCALEGNMLVPSPQGGSACKRLNLFLRWMVRCDAVDPGGWHRMDPSR
                     LVVPLDTHMHRIALGMGLTRRRQADWRAVVEVTEAFAALVPEDPVRYDFVLTRPGIWA
                     SHGRSRAGGGGAALLSKEGLDFAGD"
     gene            14491..15324
                     /locus_tag="PLM79_09980"
     CDS             14491..15324
                     /locus_tag="PLM79_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV86680.1"
                     /translation="MALKWAKDAEEAVSKVPFFIRRKVRKRVEEEAVREGAREVGLNH
                     VLACKQRFLRNMDREVRGYRLEACFGKDGCPNRAVEDDGTLDRLETLLAQKNLRDFLV
                     SRIQGPLKFHHEFRVSVSYCPNACSRPQITDVGLMGARLPALSCEPCTGCGSCIEQCR
                     EEAVAIPADSRTPTLDFPRCLACGQCIAACPTGRLQPGKIGYRILLGGKLGRHPQLGR
                     ELPGVFTAEETLEIVRECLDHFIRHNEGGERFGEVLNRVDLPGEIWEKYGHRTSEPCR
                     E"
     gene            15353..15670
                     /locus_tag="PLM79_09985"
     CDS             15353..15670
                     /locus_tag="PLM79_09985"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV86681.1"
                     /translation="MKKVSLSETDQYAENAFKRFIVHDSPYFKILNFNFRAGQELPVH
                     SHEIEGQVSILILEGEGEFLGKDGAVLPARPGDVLISDISEPHGVRAQSDLRVLVTIA
                     PPI"
     gene            15696..16811
                     /gene="lpxK"
                     /locus_tag="PLM79_09990"
     CDS             15696..16811
                     /gene="lpxK"
                     /locus_tag="PLM79_09990"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOV86682.1"
                     /translation="MFRTWGAKTVRMLGAWVSSRWYEEAPCAFPPTEWALRAAAGVYR
                     AGLSFHQGILRRRARSLPVPVVSVGNLVAGGTGKTPFTLWLALFLKDLGWSPAILSRG
                     YGRRGGDTCRVQLGGNPDLWTRQYGDEPVLLARRAPEVPVWVGKDRRQSGQAAMGRGA
                     DVLILDDGFQHLALQRHLDFVLMDAERPWGNGRLLPWGPLREPIEHLERADALVLTRA
                     RKTADPGSPTPSPGFPLPPRPVFSCRYRLAGFRTGFSGGLLSPESLRGEGVIAFSGIA
                     RPDSFFDSLRAVGIHPLRCLPYADHHPYREAESRAILENARKLCASFLVTTEKDWVRL
                     PRELGQVVLAAVLEIDFGSDLPRIRAFLRNRLPPPTG"
     gene            16918..17991
                     /gene="rtcA"
                     /locus_tag="PLM79_09995"
     CDS             16918..17991
                     /gene="rtcA"
                     /locus_tag="PLM79_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HOV86683.1"
                     /translation="MIRLDGSLHSGSGTLLRYAAALATLTKTPVHMVRIRSRRPRPGL
                     RAQHLQALRACASLCSGQLEGDEVGSQEIYYQPGPTLEGGDFHWDVGTAGSTTMIAFT
                     VLPVAVFAPVACRFTITGGLFQDFAPSAFHMQKVLLPLLETMGVRAQLEVVRPGYYPE
                     GKGILRMTVAPGPPSLAPLSLEQQGTIREIRGIALASHLERERVAERIAVRCRELLER
                     EGWSSTIRITNDGTAVRKGAALVLWGESDTGCRWGADRAGKKGRRSEDLASHAVGSIL
                     EDLRSGACTDRYTADQLILFCAVARGTSQYRIPRVTEHVQSNRWLVQTILGVESRLSG
                     NLLTIEGGGLQRGVAQLGAGVVK"
     gene            17988..>18656
                     /locus_tag="PLM79_10000"
     CDS             17988..>18656
                     /locus_tag="PLM79_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86684.1"
                     /translation="MSSRIRIRGIYSTSLTKLLLDGGYRMVDPSSRIRERFGIEETTG
                     PHDILVVDRDDFQGIELVGGQPEKLCQFLTFLQERLVDATLLEFGPSTDDDTEVRARL
                     EFPGGTKEFLDEVRGAVVPTVKRHHRLRIAEAKGLEAAEDALPGHPEKRESLERELFR
                     EAVLLPLEKAGRVRLEHIRPSGRPMRPREGKVLWVDGDRILFQRRFSKGRYDGLNLPI
                     QPGDY"
BASE COUNT         3879 a         5522 c         5828 g         3427 t
ORIGIN      
        1 acagcaggct gaccttgccc ttgaacgccg ggtcccacag gtcctcgatc ttggcctccg
       61 ccccttccgt ttcccgtcga acgggtttgt tggccaccct ggcctcgatg tagctcacgt
      121 tgggatcgaa cttctggtag tagtgggcaa ggaccgggtt tctctgcatg gccggaaggg
      181 tgtctcgccc cccaaaccac ccggcagcca ggtcgaagtc caggagcccg atccgggaac
      241 ccgaatcctc cacgtggatg aaggggagca gtctcagcag gtccggcgcg ttgtctatgc
      301 cgtagatctt cttcacgtcc tgggaggtga cgacgatggg ccgttcaact ccctggaggt
      361 cgaagtccag gtagtgggcc aggatcgagg gaggaagatc gttttccgta aggctggggg
      421 actggttgaa gatctcggcg gtgagcagga gttccatgat ctgcattaca tcgggatttt
      481 cggcagccat gttgatgacc tcggcaatcg gtggaagtgg aacggcggat gagaagatga
      541 acctctgtcg cggttttcat gaaacaccgt tcgggcgtac ccgcctcgcg ggccctggga
      601 acgcgtttgt ctaaagacgt aggcggaacc ttgaatcttt tccgatgaag tttctgcgta
      661 ccgcaatcct ccggactgta tgccgaaatg atcttgctgt caatgcaaac tcccgccccg
      721 gccgggcgcc cggacgccgg gacctcgaaa ccagggccga catgggcgga cacggccctt
      781 gtcccccgac ccgccgccgg atacaaaaaa tactttggca gggtcgcaat cgatgctatt
      841 ttcttgcgcg ctcaatctgg agaacaccgg atcttcctca gcgatcctgg tctttgggaa
      901 ccgggacccg gggagtgcct tttctcgggg aacggcgctc attgggccaa gggacaccgg
      961 attatcaacc gacatgaccg gcacgaacac gggagatttg cctgggatga atcaagatcc
     1021 gcctggcaaa caggttgagc gactgctgga ggccctggtg gggggtgggg tttccctgga
     1081 tcgagatcga accgatctct cgcgcctggt ccgggatgcc cgaagcttcc aatgcaggga
     1141 catccgtgtt gtggcattcg gaggcggtac ggggctttcc acggtggtgg gcggaaattc
     1201 ccagctcgag gattggcccg aaaacccctt catcgggttg aaacaggagt tccccttcct
     1261 cgatgtggtt gtctgtacca ccgacgacgg agggtccacg gggctgcttt tgcaggagct
     1321 gcccatgatc ggggtcggcg acctgcgcaa gctgttgctg tcgatgattc tcgaggaccg
     1381 gcttcgggaa acctacggcc tggacccgat cgaaacaagg cagctggtgc gactcatcca
     1441 cggcctcttc aatcaccgct tccctcccaa ccacaacccg ttcaagtgcc tttcgaaccc
     1501 cctcctggtc gtgccgcgaa gctggagaaa atcctgcccc gaatccctgg ctcgactgct
     1561 ggccaccctg ggccaatacg tggccccggg aggggacggc ccgaccctga agccgggagg
     1621 ccattgcctg ggaaacctga ttctcacggc ggccatcctc cgggaagcct cgatccgcgg
     1681 tgtggatccc cccgacgtgg aatccatcga gaagggactc gacaccctgg ccgaggtcct
     1741 gggcgtggtc ccgggccgat tgcatccggc cacctccacc cccggtcagc tggtgctgcg
     1801 atacagcaac ggggtcgccg ttcgagggca acggaaatcg gcggtctgca ggcgggactt
     1861 ccccatcgat tgtgtggccg tggaattctt cggcaacccc gcggtgaaac aagacctgtg
     1921 caggaccatc cgggaggcgg acctcatcct gttcgccccg ggaagcttgt acacaagcat
     1981 tctgccgatc ctgcagctga cggatgttgt gactgccatc aaggagaacc ggaaggccct
     2041 gaagattctg ggagccaact tctggatcca ggagggggag accgacatct ccctgcgggg
     2101 caggaatcga gggttcaccg tgtccgaact ggtggaagct tacgaccaga acgtgcccgg
     2161 cgggtcggcg ggcctctttg acgtggtgct gagcgccaat ctcgagcacg tgcccggcaa
     2221 cgtgctcagc aattatgccc tggaaggcaa gagccccatt tacctggacc ggagccggct
     2281 ggccgggttc ggcgtgcttc ccgtggaggc caccatttac tcgaggcggc gtttgcgggt
     2341 ggctcacgtg atccaccacg atcccgaaaa attcgccctg gcggtccggg gcctggtgtt
     2401 tgcccacgat cggttcaacc ttcggtgcga gtcccttcgc ccgcacgctt cccggccctt
     2461 gcggcaatgc gcacccgtgg gaatctccgc tctttgcgac tacaggaagg ccatgagccg
     2521 agagttggac ccaaagcgct tcctgccccg gcagcttcgg cgggtcttcg atgagatcct
     2581 ctgggaaaac cgggacattc gtcccggaca tctccgaaac ttccgggggg cgcggatcat
     2641 cccctcggcc cggtggcacc ggagcaacga gtgggacaac gtcctgggct actacgaccc
     2701 ggaagataag atgatctatc tccatgagca gttgcgcaac gatcccgagc gactcaaggg
     2761 gaacctgctg gttgcgctgg gggagtcgtt gctgggacgg tatctcgagg gtcgccggtg
     2821 gatcgattgg gagtcccggg gcgagtgggg ggcaaggtgc tacgaaatcc ggctgcggcc
     2881 ggaagaggag cgggaatgtt tcctgggcga cgcccagctc aaggaatacc tgcgcctgtc
     2941 ccgcatggtt cccaaccccg aagacccgag gacctaccga atcacgctca acggcaacga
     3001 aggcttcact cctcccggcc tgctctttgg ccttctctac gcatggtatc tcaacaacga
     3061 ttacggtgaa atcctcgaat acgagatgtc cctgttgcgt cgacctcccc agaagctcat
     3121 cccttgtcag ctccaggagc gcgatcgaaa acaggcactc atcgccttgt ttcgcaagga
     3181 ggtgttcgaa cacgcggagt gaatgaagga aagggtcgag tttgaacccg gattgagcga
     3241 tcggactggg acgaatcccg tttcgtcgtt cccgagcatg cgggaatccg gaaaaggcgt
     3301 gcatacccgc tcgccttggc gggcatgacg cccggatgct ctcgggattg ctcgatccag
     3361 gtttgaactt ttgaaatggg ggtccggtcc ggaattggag gagaaaatgg aacttagacc
     3421 caggagtcat cagaagattg tcgagctcat ccaaaagggg gtggagatcc cgaaccccct
     3481 gaccttggac atcggggacg aggtgctccc ggagaacatt tccggcaacg gggtcaagat
     3541 ctatcccggg tgtcgcatct acggcaagga aaccgtcatt gccgggggcg ccaagatcgg
     3601 agccgagggc cccgtcacca ttgacaactg ccaggtcggt cccatggtcg agctcaaagg
     3661 cggctatttc aagggttccg tctttcttgc caaagccaat atgggaatgg gcgctcacgt
     3721 gcgggacgcc tgcatcctcg aggaagaggc caacggggcc cactgcgtgg ggttgaaaca
     3781 aaccatcctg tttccttttg tgaccctggg cagcctcatc aatttctgcg actgcttcat
     3841 ggcgggggga agaagcagga aagaccacag cgaagtggga agctcctaca ttcacttcaa
     3901 ttacacgccc gacggagaca agtcgacccc ttccctcctg ggcgacgttc cccggggggt
     3961 catgttgaac cagcccccga tcttcctggg aggccagggc ggcctggtgg gtcctcttcg
     4021 gttgggcttt ggaaacgtgt ccgtggcggg aaccatcctg cggcacgata ccccggaaga
     4081 ccgaaagaag atcatcgggc tgacctatcc gcccaccgtc gtggattttg tcccctaccg
     4141 gtatcccaat ctcagccgaa ttctcaagaa caacctcatt taccttgcca accttgtcgc
     4201 cctggaatgc tggtaccgga acgtacgcaa gccgtttttt tcgaccatgg aatatggaga
     4261 gctgttctac cggggagtcc tggacaaact ggccgcagcc aaaaaagaac gaaccggcag
     4321 gctcaaggcc atggtccaga aggtccctcc ctcccacgac caatgtcgat gcggaaagga
     4381 gtcggaaaag gggtgcgaat tgcacgccaa tatcgacgcc gtttgcgaac tgttcgggca
     4441 gagcctgtcg gatccccggg aggcccagct tgccgaaggt ttcctgaacg ccttcgagaa
     4501 atttcgcggc gccaacggcc gggactacat ccaggtcatc caggccatgc cggaaagcct
     4561 gtccgccgaa ggggtccggt ggcttcaata cgtagtggat gccttgtgca ggagggcagg
     4621 agcgcttctt tcctcctttg gccttttctg aagcaccgat tgttctgaat acatgatcaa
     4681 ggaaggacaa aacatgggta ctttattcgg tacagatgga atccggggcg ttgcaaacca
     4741 atatcccatg gacgccgcca tggcttttgc cgtaggacag gccgtcactc atgtgctcaa
     4801 aagggaatcg caccggacgc ggatcatcct gggaaaggac acgcgcatct cgggctacat
     4861 gctcgaaagc gcgctcctgg cgggcatcac ttccatgggc ggcgatcctt acctggtggg
     4921 tgtgctcccc accccgggga ttgccttcat taccgaaagc atgcgcgccg acgcgggaat
     4981 cgtcatttcc gcttcccaca acccctacca ggacaacggc atcaagattt tcggggggaa
     5041 cgggtacaag ctttccgacg aacaggagga agtgatcgaa aagctgatcc tcgagggaga
     5101 cctgcccaag aaggtcccgc cccctcacca aatgggacag gcctaccgca tcaacgacgt
     5161 ccacggccgg tacatcgtgt ttttgaagaa caccttcccc cgggccctgt ccatggaggg
     5221 gatgaagatc gtgatcgacg cggccaacgg agcgacgtac cgggtcgccc ccgaaacctt
     5281 cacggagctg ggcgccaagg tggacatcat tcacaacact cccaacggga tcaacatcaa
     5341 cgaccgctgc ggctcccagc atacccagga cctccgaacc cgggtggtgg aaaagggcgc
     5401 cgccatcggc ctggccttcg acggagacgg ggaccggttg atcgccgtgg acgaaaaagg
     5461 ccgccagatc accggagacc agatcctgat catctgttcc aagatgctca aggagcaggg
     5521 ccgactgaag aacaacctgc tggtaagcac ggtcatgagc aacctgggac tgacggttgc
     5581 ctgcaggaag tacgggttca ggcagcacgc ctccaaggtc ggcgaccgct atgtgctcga
     5641 ggatatgcag cagttgggcg gcgtcctggg cggcgaagaa tccggtcatg tcatctttct
     5701 cgatcaccac acgacgggag acggcattct cacggccctg cagctcatcg cagccatgct
     5761 gtcctcggga aaacccctct ccgagctcgc cggtttgatg gatatctttc cccagaaact
     5821 gatcaacgtg aacgtctccc gaaagccgga actctcgacc ctgcccgagg tggttgcggc
     5881 aatcaaggac gtggaatcgg ccctgggcga ccagggcagg gtcttggtgc gttattcggg
     5941 cacccagaac atgtgccggg tcatggtgga agggccgacg gacgagatca ccgcaaagta
     6001 ctgtcagcag atcgtggacg cagtgaaggc ggccattgga tgacccaatc aggctacgcg
     6061 gtacggaccg gatacgaggc cgggagcacc cggggagatt ccgttatcgg aaaggattct
     6121 acatggcttt caccatttac gtaaccagcg actgggacca aatgagccaa gtcgccgccg
     6181 agttgattga atcggacata cggcaaaagc agacggcgaa gcgcgagtac gtgctgggcc
     6241 tcgccacggg gaattctccc accggtattt acaagcatct tgccaaagcg ttcaatgccg
     6301 gacgcatcag cccccgcacc atccgcacct tcaacctgga cgaatacgtc ggacttcccg
     6361 gggaaaaccc ccagcagcgg gccctccatt gcgaatccta cagctacttc atgatctccg
     6421 aattcttcgg actcttgcgg gagaagtttg cggaaaccaa cgttccctgg ggaaccctcg
     6481 tggaccagga ccacctggtc cgggccctcg aagaccacaa ggactgctat gagttccagg
     6541 gcacggatgc aggcaaagcc atcgtcatcg gagaccaggc ccagggatac ctcaagacca
     6601 tcaaggagga gatcctggac gcctactgga agaagatcga tgcctgcggc ggcattgacc
     6661 tgcaggtcat cggcgtggga ggacggggac acgtggcctt ccacgagagc ggcattccct
     6721 tcgaaaacaa caaggtgatc ctggtcaagc tggatgagaa cacggtggcc aacgccgtgg
     6781 aagacggcca ctttgcgagc agggaagaga gtccccggta tgccgtctcc atgggtgcgg
     6841 aacaggtcta ccgggcgaag acggtggtgc tggtggccaa cggatcccgc aagaccggac
     6901 ccgtgaccga ggccgtcctg ggagaagtca ccccggatgt tcccatcagc tacggacaga
     6961 agtacgcagc caggggcggg aatatggtct atgtcctcga tgaggtcgcc gcagccgagg
     7021 tcttgaagcg atccgccgag gtgaaagcca agggctaccg gatcgtggat aaacgggggg
     7081 aaccgtttgc gaaggtcgag tctttgacct ttatccggga cgtccggaca ggccgactgg
     7141 ggtagcgaaa ccgacctgca gaacctggag ccggccgggg aagacggtcc ttgagcccgc
     7201 cccggccggg ccctttcggg gttgatctga tcagtgaagt tccttctccc ccgggaagag
     7261 gattggggtg agggaggatg gcctctgaaa gccccctcac gcctaccctc tccccccgag
     7321 gcgggggcga gagtagtttc gtcggtcctc aagacaaccc tattgtcgct cggcccatcc
     7381 ccccctgctc gccccaccgg ccccaacgtg aggagaccga cggggattcg atcggggttt
     7441 ccggaggagg atcgtcgggc aagaaggcgg gcgctttcct cgatcccccc ttttggcgac
     7501 ctcggggtgc tttgtgcctc tcgagggaat agacccgata cggaccccca cgggcgccct
     7561 cccggggcga ccattggccg cgaatgggcg gcgggcagac ttcgaaaggg cttgggatga
     7621 atgacggcct ggagcaaaag attgtccggc tcctggaaga aaagggtcct ttgacggggt
     7681 ccgagatcct ggagagaatc cgggagagga attttctgct ttggaggacc tgttatcttt
     7741 ccagcaagct ggacatccgg accctgggta cccggtatct gagactggac cagaaagtgg
     7801 acgggtacgc ccggctctcc ccctccatcc tccgtgagtt catgacgtat accttggtgg
     7861 gggtttccgg ggcccgggag ggtctcgacc ggagagccga ccgaattctc cgacacatcc
     7921 gggaagtgag ccaatccaag ttcgccctgg ctcactcgat agccacccgg ctgcagaacg
     7981 acctgaacga ccgatggccc cgggaaggac ggttgtgttt cattcttgcg ggggacatcg
     8041 tctacgacat ggcccacgat gttccccgtc ccgaggccag cacgggtgag ctggtcaacg
     8101 gatccgacat cgatttggtg gtggttgccg acgacaccgt gcccgacgac tttctcgagg
     8161 ggctcgacgg ggccatttac cgggaaaaat accgcaccct catctccccc ggcgtccggg
     8221 aagaactcga ctacgtagtg aaaaagatgg aacgggtgag agaacagctc ggcttcgata
     8281 cgttcaaacg gatggttgcc tgcaagatcc tccgggaagg agcgttgctt ctcggcagtg
     8341 aaagcctttt ccgggagatc aagaccctgc ttgtccggtc gggggtcgtg gagaagctcg
     8401 aggagatgga aaagcaggcc ggggccttga gacgacgggc ggaggagcac ctgctgcaaa
     8461 cggacccctc tcgaatcgtc gaggaggact tgaaccattt ccacaccagc gaagagacgg
     8521 aagaattcga ataggtccgc cgcctgccgg ttggatgttg cagagtcacc atcgccgtgg
     8581 ttcgaccagc atgttgccca tgggcgatcc tggatcaaag ctcgacctgc agacccagct
     8641 cgatcacccg gtcatgagga acactgaagt aggccgttgc cgtgcgcgca tttcgggaca
     8701 tgtaggcgaa aagcgccttg cgccacctct gcatcttcga atccccgccc gtgagcaagg
     8761 attcccgact caagtaatac gtcgtcgtgg cgggttgggt ggaaagcccc tggagggagg
     8821 cgagctgcat gatccgggga acatccggtt tttccgcaaa cccgtaaaaa gcaatgatac
     8881 ggaaaaaccc ctggcccaga tcctcgatct ttaccttttc ctcgtccggc aacacgggaa
     8941 cgtccacgga acggatggaa agaagaacca ccttttcgtg cagcatctgg ttgtgcttgt
     9001 aatggagaag aagagagctg ggaatgccca cgggggagac cgacaggaac accgccgtcc
     9061 cacggactct ctggggatcg tgtctctcca catcctccag gaaaagatcc acgggcagcc
     9121 gggaactgag catcttcctg gccaactcgg cacgaccgtc cttccaggtt accatggccg
     9181 tggcaatgac ggccgcaatg gtcagggtca cccaccctcc gtcgaacagt tttaggagat
     9241 tggcgctgaa gtaggagaga tcgaaagtca gaaataaggc caggagggga agggccttgg
     9301 cgagggacca gccccaggtc cttgtgatca cgaagaaata gagaatcgag gtgaatgcca
     9361 tatcggccgt tacggcaatg ccgtaggccc cggcaaggcc gctcgatttc ttgaacacga
     9421 gcaccagccc gatgcaggcg atcatcatca ggtagttcac ttcggggata tagatttgtc
     9481 cttgggtttc ccgggaagtg tggacgatct gcaatcgcgg gcacaggccc agttgaacgg
     9541 cctgctgggt cacggaaaag atcccggaga tcatcgcctg ggatgcaatg atggtggcga
     9601 gggtggccag aaaaaccatg ggatagagaa ggtaccgggg aaccagtccg tagaacggat
     9661 tgaaagccat gttcggatct tccagcagga gcgcgccctg gccgaagtag ttgagcaaaa
     9721 gggagggaaa cgccaatccc agccaggaca gtcgaatggg gttgcggccg aaatggccca
     9781 tgtccgcgta gagggcctca ccgcccgtga tgcacaggac caccgaaccc aggaccacga
     9841 ttccgtggag ccggttcacg gcaaagaact cgtaggcgtg gaggggattc acggcgtaga
     9901 ggatgtcggg cctttgaagg atttcctttc ctcccaggac ggcaatggta acgaaccaca
     9961 cgatcatgat gggcccgaag acgctgccga tgtccgccgt ccctcgatgc tgtaccagga
    10021 agagaagcag gagcacaccg caggtgaggg gaagcaccat gggtcttgcg gcctccgttg
    10081 ccacctcgag gccctcgatg gccgacagaa ccgaaatggc gggagtgatc accgcgtctc
    10141 cgtagagaag cgacgccccg aggatcccga agaggactat cgccgaatgg agctcggggg
    10201 acattcttcg gcccgcggaa gggatgaggg ccagcagggc aaaaatccca cccatgccgc
    10261 gattgtcggc acgcatgaca aaaacgatgt attttacgca aatcaccatg gtcatggacc
    10321 agaagaccag ggagagcacc cccaggacat tgccctgagt caaggccacg gcatgggtcc
    10381 cgtggaagca ctcccgaatg gaatacaggg gacttgtccc aatgtctccg taaacaaccc
    10441 ccagagaacc cagggcaagg gataaagtgg tgcgtgaaaa aagagggggc gacttgtgcg
    10501 gggaaccggg atctcccggc gacggctgcg gttcggacat attcacaacc cggtccatga
    10561 tggaaaacgg ctggaggcga cagcaacgaa aattcagcct tggcttatag tcaatctccc
    10621 ttcccaagtc caccactttg acccgaggga ggctgcagac ggttctcggg gcgatcggac
    10681 gcgggcgact ccgtcggggg gattcttcga cggctcccgt ttgtggacag ggaccggggg
    10741 gatcggcgag ccgggcatcg aggaaccgat gccggcgtcc ctcttcattg gagttgatct
    10801 ccgccgggcg gctgtggttt catcatgggg ctttagccgt caagggtttt cggggcatgt
    10861 ggcttcgtgg gctggagggt cgaaatgatg gaaaaggctg tgcctggaag aatgaatgtg
    10921 caattggccc ttctcagcgg cctgatcctg ttggtcgtcc tggttgtcgc cggtctttgg
    10981 tacaaggaca aagagcacga cctggagagc gttttccaca gtaccctcca gaagcaggag
    11041 atcgtctccc ggatgaggat cagccttctc aagtccgccg agctggagaa ggcggccgtc
    11101 atggcggaca ccgacccgtt atcccgctcc ctggccgacc agtctttgca ggcagccgaa
    11161 gaaacggacc gccaacgccg ggagttgaat cgtctcatcg aacacgattc cttggcggaa
    11221 gaggtcgcgc tgctcaagga gtttgatcat tgttggagtc agtcccgaaa gatcgacgcg
    11281 gtgctcctgg attttgccgt ggaaaacacc aatctcaagg cggcgcgact ctccttcacc
    11341 acggcgggca aaaccctgga actcttccag caaaggctta cggactttct cgggagcatg
    11401 gcctcgaacg agcgggaatg ccgggggggc aaagcggtct ttgcggccct tgcagccggt
    11461 ttcaaaatcc agtacttgca cgcgccccac atcgccgctg ccaacgacga acagatggac
    11521 gccattgaaa aggagatcga agcgtctcgg gagggggtga aaagctccct gaaggagctc
    11581 gaggggctcg cctccgaaga aaaccgggaa ttgttgaggc cggcggcgtc cgcgttcgcc
    11641 gagtttgaag cggtttgcgc cgaagtggtc cggctctccc ggcagaattc caacatcagg
    11701 tccttggagc tctccctggg tcggaagcga aagatcacgt ccgagtgcga cgagatcctc
    11761 gaaggcctgc aggcggtgat ccggaaacga accccggacg caacccgata gagtctcccg
    11821 aaggcctgta tcatgagggc ccgctgtcga gaggattggc ttgtttttcg gacatgcaaa
    11881 gaatggggcg ttgccgggag gaagagaatc ttcccttagg cctgcacgga actcggcacc
    11941 gggaattcgt ttggccccag gccctttatc ggggggcggg gcgcctgccg attgagccgc
    12001 agaaacgctc ttttgcgtgg acagcgggcg gggagtgggg taaattgccc caccgattga
    12061 attccgggtt tcatccgggg cgggaggaaa agccgcggag gagccgggta gggaagcggt
    12121 tgcctccggc cctcccgccg aggcaggtct ggtgcaacag gaaggatatc atgaggaaga
    12181 attccccctc cagcgtcttg gttctcggct tattggccct ggctgtgctc ttgaactgct
    12241 ccggctgcgc aacctcgccg tccacgaaaa cgggaaccgg gacgatgtgt cccctgactc
    12301 cccccgtcga acccacgtcc gtcggtccct ccaagcccga agccgtggtg ttcatggttc
    12361 cctaccacgc ccctcccaag aagatcgacc tgtgcgggga accggttccc accgaaagac
    12421 aggaggtgct ggaacggttg gacaaggaat tcaccctcat cgtctacaac catgcccagg
    12481 tctatctgtg gctcaaacgc atggagcgct attttccctg gatcgatgag cggctgagcg
    12541 cccagggtct tccctcggac ctcaagtacg tggccattgc cgaaagcgat ctgcttccca
    12601 acgcctgctc tcccaaaggg gcggcgggac cctggcagtt catgcccgcc acgggccgaa
    12661 actacggatt ggatcagcgg ggcgactgcg atgaacgtta cgatttcgag atggccaccg
    12721 aaagcgcctt ccgttacctc cgaaacctga acaaccggtt caagagctgg accctggcca
    12781 tcgcggccta caactgcggg gagggccgga tccaggagca aatgaggacc caggggaccc
    12841 gggatttcta ccagatgaag ctgccccagg aaacggagcg gtatgtgctc cgaatcctcg
    12901 ccatcaaggc cgtcctggga agccccgagc aatacgggta ccatcttccc cggggagaag
    12961 gatacccgcc cttcaaggtg gaccgagtca aggccacgtt ctcgaggacg gtttccattc
    13021 aatcggtcgc agccgccgcc ggaaccaact accgggaatt caagcggctc aatccggttt
    13081 tccggggaga cgacatctct ccgggcacct acgaactcaa gctcccccag ggaagcggag
    13141 cgatctttca gaagaatcac gcggccgcgg caacctcgac cgcggacgtt tctcccgcaa
    13201 gtccttccaa aaccggtgtg agctccgaag atagaaaggc cgccgacgcc cggggcagaa
    13261 gcgactccgc caaaggccga tcggcgcgcg tccacgtggt gaagaaggga gagaccttgt
    13321 cggggattgc caggagccac caggtgagtg tccacgagct gcagcgggcc aacaagctca
    13381 agggcagcgt cgtgactccc ggccagaagc tgcgcattcc ctgaggaatg ccccccggga
    13441 agcatcccgt agtcaatccc ctgcgaaatc gagcccctct ttggagagca gggcagcccc
    13501 tccgcctccg gccctcgacc ttccatggga agcccaaatc cccgggcgcg tcaggacaaa
    13561 gtcatatcgc accgggtcct cgggaacgag ggccgcgaag gcttcggtca cttccacgac
    13621 cgcacgccaa tccgcctggc gacgccgggt cagccccatg cccagggcaa tcctgtgcat
    13681 gtgggtatcc aggggaacca ccagccggga cgggtccatc cggtgccagc cgccgggatc
    13741 cacggcatcg catcggacca tccaccggag gaaaagattc agccgcttgc aggcgctgcc
    13801 tccttgggga gacggcacca gcatgtttcc ctcgagggcg cagcccaacc gtccggcgaa
    13861 atgggagagc gccgcacaca ggcttccgtc ggcttcttcc caggcccgga cgaaaaaact
    13921 ctcgagggag ccgtaacgga tcacggcttc tcgaatcccc cccaggagtt ccgaaagatg
    13981 gtctccggtg gtgaatcggt gcttgaatcc ctcgaggatt tttcgaaatt cccgacggtc
    14041 atgccgaaga atgaacgttc gcgggctggg gcccagcctt tccaggaccc gggagacact
    14101 ggacaggact tgggaaaccc tgccgtacgc caggcaggaa gcgagaattc ccacgatttc
    14161 ccgatccccg ggatcggaat acccgtagag aaactcgagg gggtccggat gaacgaattc
    14221 ccggcggttc catttttcgt agaggtcctc gaggccttcg cgggtgaagg gaggaacgaa
    14281 atccgggaga ggatcgggag cgcgatcctt catcgcgcct cgatcctcga ggtttcggac
    14341 atggggtggg tccggcaaga tttcacctcc tcccttgact tgggtcaaac acagccattc
    14401 ccggtaacta taaactgggt ttggcggcag acaacccgcg aaggacggat cgcttgccgc
    14461 ggagcatgga ttttggccgg gagatgagtc gtggcgttaa aatgggcaaa agatgcggaa
    14521 gaggcggttt cgaaggtccc ctttttcatt cgtcggaagg tacgaaaacg ggtggaagaa
    14581 gaggctgttc gagaaggtgc ccgggaagtg gggctgaatc acgttttggc atgcaagcag
    14641 cgattcctgc ggaatatgga ccgggaagtc cgaggctacc ggctggaagc ctgctttgga
    14701 aaggacggct gccccaaccg ggccgtggaa gacgacggga ccctagatcg cctggaaacg
    14761 cttcttgccc aaaagaatct ccgagatttt cttgtgagcc ggatacaggg tcctttgaag
    14821 ttccaccacg aattccgggt ttccgtgtcc tattgcccca atgcctgctc gagaccccag
    14881 atcacggacg tcgggctgat gggggcccgg ctgcccgccc tctcctgcga gccttgcacg
    14941 ggctgcggat cgtgcatcga gcaatgccgg gaagaggccg tcgccatccc ggccgattcc
    15001 cggaccccga ccttggattt tccgcgctgc ctagcctgcg gacagtgcat cgcggcctgc
    15061 ccgacaggga ggctccaacc ggggaaaatc ggctaccgga tccttctggg aggcaaactg
    15121 ggcagacatc cgcaactggg tagagagctg cccggcgtct tcaccgccga ggaaaccctg
    15181 gaaatcgtcc gggaatgcct ggatcatttc atccggcaca acgagggcgg ggagcgtttc
    15241 ggagaggtgt tgaaccgggt ggacctgccg ggggaaatat gggaaaagta cggacatcgg
    15301 acgtccgagc cctgccgcga atagacggac tcggagaaag ggaaggaatg ccatgaaaaa
    15361 agtgagtctc agcgagaccg atcagtacgc ggagaatgct ttcaagaggt ttatcgtcca
    15421 cgattctccg tactttaaga tcctcaattt caacttccga gccgggcagg aactacccgt
    15481 tcattcccac gagatcgagg gccaggtctc gatcctcatc ctggaaggag aaggcgaatt
    15541 cctgggcaag gacggggccg ttctcccagc ccgcccggga gatgttttga tcagcgacat
    15601 cagtgagccc cacggcgtgc gggctcaatc ggatcttcga gtactggtga ccatcgctcc
    15661 gccgatctaa tccacggaca ggcaggagga gatccttgtt cagaacctgg ggggccaaga
    15721 ctgtccgtat gctgggcgca tgggtttcaa gcagatggta tgaggaggcc ccctgcgctt
    15781 tccccccgac cgaatgggcc ctgcgggcgg ccgcgggagt gtaccgggcc ggcttgtcct
    15841 ttcatcaagg gatcttgcga cggcgagccc gatccctgcc cgtcccggtt gtgagcgtcg
    15901 gaaacctggt ggcgggaggg acgggaaaga ccccgttcac cttgtggctc gccctgttcc
    15961 tgaaggactt gggttggtct ccggccatcc tgagtcgggg ctacggacga aggggcgggg
    16021 acacgtgccg ggttcagctc ggcggaaatc cggatctttg gacacgtcaa tacggggacg
    16081 agcccgtgct cctggcccgc agggccccgg aagttcccgt gtgggtggga aaagatcgca
    16141 ggcaatcggg gcaggcggcc atgggtcggg gagccgacgt gctgatcctg gacgacggtt
    16201 ttcaacacct ggccctccaa cgtcacctgg atttcgttct catggacgcc gaacggccgt
    16261 ggggaaacgg ccggctgttg ccctgggggc ctttgcggga gccgatcgaa cacctggaac
    16321 gcgccgacgc cctggtcttg acccgtgcgc ggaaaaccgc cgaccccgga agcccgaccc
    16381 cctcccccgg cttcccattg cccccccggc ctgttttctc ctgccggtat cgcctggcgg
    16441 gatttcgaac cggtttttcg ggagggctgc tttctcccga gtcgctgagg ggggaggggg
    16501 tgatcgcctt ttcgggcatt gcccggcccg attctttttt cgactctctc cgcgccgtgg
    16561 gaattcatcc cctgcggtgc ctgccctatg cggatcacca tccctaccgg gaagcggaat
    16621 cgagggcgat cctcgaaaat gcacgaaagc tttgtgcctc cttccttgtg accaccgaaa
    16681 aagactgggt gcgactcccc cgggaactcg gacaagtcgt gctcgcagcg gtcctcgaga
    16741 tagacttcgg gtccgacctc ccgaggattc gggcgtttct gaggaaccgt cttcctcccc
    16801 ccaccggata gcccgccgga aggtccttcc attttccatt tttgggaaac gtcccatttg
    16861 tactatgatg cgagggggaa atcgtcgcag gtccggaaga atgccggggg tgatcgagtg
    16921 attcgactgg acggttcatt gcattcgggc agcgggaccc ttctccgcta tgccgcagcc
    16981 ctggcaacct taacgaagac gcccgttcac atggtgcgga tacgctcccg gaggccccgg
    17041 ccgggactca gagcccagca tcttcaggcg ctgagggcct gtgcctccct ctgctcggga
    17101 cagctcgaag gagatgaagt gggttcccag gaaatctatt accaacccgg acctaccctc
    17161 gaggggggag atttccactg ggatgtgggc accgccggat ccaccaccat gatcgcgttc
    17221 acggttctgc ccgtagccgt gttcgctccc gttgcctgcc gattcaccat caccggcgga
    17281 cttttccagg acttcgcccc cagcgctttc cacatgcaga aggtgctcct ccctctcctc
    17341 gaaaccatgg gggtccgtgc gcagctcgag gtggtccgcc cgggttatta ccccgaagga
    17401 aaggggattc tgcgcatgac ggttgctccg ggccccccct ccctggcccc actctccctg
    17461 gagcagcagg ggaccatccg ggaaatccgg ggcatcgccc tggcctcgca cctggaacgg
    17521 gaacgcgttg cggagcgcat agcagtacgc tgccgggagc tgctcgagag ggaaggatgg
    17581 tcttccacca tccggatcac gaacgacggg acggcggtcc ggaaaggcgc agcccttgtt
    17641 ctgtgggggg aatcggacac ggggtgccgc tggggagcgg atcgagctgg aaagaaggga
    17701 aggcgatccg aggaccttgc ttcccacgcg gtcggctcca tcctggagga cctgcgctcc
    17761 ggggcctgca cggaccgtta tacggcagac cagctcattt tgttctgtgc cgttgcccga
    17821 ggaacctccc aataccgcat cccccgggtc acggaacacg tccaatccaa tcgatggctg
    17881 gtccaaacca tactcggggt tgaatcccgg ctttcgggaa accttcttac aatcgaaggc
    17941 ggggggttac agcgcggcgt ggcccagctt ggggcgggag tcgtgaaatg agctctcgaa
    18001 tcagaatacg aggaatctac agcacgtccc tcacgaaact tctgctggac ggaggatacc
    18061 gaatggtcga tccgtcctcc agaatccggg aacgcttcgg tatcgaggaa accaccggcc
    18121 ctcacgatat cctggtcgtg gatcgggacg acttccaggg tatcgagctc gtaggcggac
    18181 agccggagaa gctgtgtcaa tttctgacgt ttctccagga acggctggtg gacgccactc
    18241 ttctcgagtt cggcccttcc acggacgacg acacggaggt gcgtgcccga ttggagtttc
    18301 ccggagggac caaagagttc ctggacgagg ttcggggagc ggtcgttccc acggtaaagc
    18361 ggcatcatcg actgcgcatt gccgaggcca aggggctcga agcggccgag gacgccctgc
    18421 ccggacaccc cgagaaacgg gaaagcctcg agagggaact tttccgggag gcggtcctcc
    18481 tgcccctgga aaaggcggga agggttcgac tggaacacat ccgcccttcg gggcgaccca
    18541 tgcgtccccg agaaggaaag gtcctgtggg tggacgggga tcgcatcctt ttccagcgtc
    18601 gcttttccaa aggccggtac gacggtctga accttcccat tcagccgggg gactac
//
LOCUS       DAOOVC010000067        18516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2043_length_18516_cov_6.041168, whole genome shotgun sequence.
ACCESSION   DAOOVC010000067 DAOOVC010000000
VERSION     DAOOVC010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2043_length_18516_cov_6.041168"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PLM79_10005"
     CDS             <1..601
                     /locus_tag="PLM79_10005"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV86685.1"
                     /translation="KPRFISGTRAFVPNGLLCRDGPGAPGPAIDAGSASPSPQRHGES
                     CKMETLKSYIDEKQQAFIVCSQCGARRIVDASKMGSLSEIKAKCTCGFVFLVSFERRY
                     FYRKKVNLRGEYLRPPPDKSWGEVLVVDISRTGLAFRRVSGSPLKTGETVKITFTLDD
                     PAQSVISKNVIVRRVDGDKIGAEFVDRNLGKALGFYLMP"
     gene            643..2310
                     /locus_tag="PLM79_10010"
     CDS             643..2310
                     /locus_tag="PLM79_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HOV86686.1"
                     /translation="METLYRFRKIDPQAYDDLRLQVSREYTQVKMRDFFLGKPVPVEI
                     YLPMMGRSETLSIEKVVHKDQVYEPSLHRRLAEEGIHTAFIRHEDETAFLNYFSEQVR
                     ETLHSQDLPSEKKTQLLYDNAEILIKKVFRERPTFAGVSMGRNLVESFAVHLSVDSVP
                     SQALLSIFSKDYYTFTHSIQVAILGMSFCRFLGWSKEETVDFGLGGLFHDIGKSAVDD
                     KILNKPGKLDKDEFELIKRHCVLGVEQLKDAHAMSREQLRVVLHHHEGMDGSGYPDGI
                     EGPEIHRYARLARIVDCFDALTTKRPYKDAMPPAEALELMRREMSRVLDPHLFKAFAS
                     FLHLEDPVPEVFQSQRMNIELGTRVFMEGTGGVQVKTSLVGMDPDRYLIVKPPKGAGA
                     VQPFCLGVDIICRYFHSGYVYGFRSRILHRTFTPFALLFLSYPKAVETVNLRKEPRID
                     CFLASEAVIAGVKHPGVIADLSTGGCRFVVGQDRDLGLPQVKVEDELIIHTRFAGDAE
                     PKALPGRVRNLRMEKEKVILGVEFENLGPETVLHLEKCVREVLDLIL"
     gene            2564..3361
                     /locus_tag="PLM79_10015"
     CDS             2564..3361
                     /locus_tag="PLM79_10015"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOV86687.1"
                     /translation="MTLPGRKLTPTSSIESVDYSLLREYLDSRAHRADDFLQKSLLGE
                     LIGKYVDLERRVDALLKNTLPATVAEEIKHSGTFSPRSYDCSILFSDIAGFTRLAERV
                     PGEVLLQMIDALFRGFDEIMERFGGTKIKTIGDAYMAVFGAPEPLENHAVQAVKTALA
                     FQDFVQRFRGARDLDLGLRIGVHSGSVMAAVVGKERMQFDVFGDNVNVASRMESAGEI
                     DRVNVSHEVYLRAADAFIFRERGEIPLKNRGLMRAYFVEGERKDVPQ"
     gene            3348..4169
                     /locus_tag="PLM79_10020"
     CDS             3348..4169
                     /locus_tag="PLM79_10020"
                     /inference="COORDINATES: protein motif:HMM:NF039346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV86688.1"
                     /translation="MCPSDKNLYDRPVLIAPGVYWVGYQDTIYNMHSNPYLVVEGDEA
                     VLIDGGSRPDFPVVMTKVLQTGLRIEQIRAIILHHYDPDLCGSLPNILDLLGKKSVQV
                     LSAEANHMFIAYYLAKEHHPLLTPTEAYPEGYDLNGRTLRFYPTPYAHAPGSFITYDT
                     RTKTLFTSDLFGSLSRKWDLYAELAPECEQCPDYEHCPNGRDYCPLPDILDFHRQVMP
                     TARAVRMAMETIRGLDVEILAPQHGSVFARRSDIDFLCRMLAGMDRMGIDERAGG"
     gene            complement(4216..4689)
                     /locus_tag="PLM79_10025"
     CDS             complement(4216..4689)
                     /locus_tag="PLM79_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86689.1"
                     /translation="MLPGEARKVEELMGAMALLEQEMAVFYESCAEILGEDEALWRDL
                     AAAERLHAQFLQEMKALLKSDPSHFQVGRPMNPVAVRTVIQGVRDNLKKLKNQELTQK
                     KALFLARDLERSILESQYAAIVKTNHPEYQKALHKILTDTRSHQESLEQQIRGRP"
     gene            complement(4838..8119)
                     /locus_tag="PLM79_10030"
     CDS             complement(4838..8119)
                     /locus_tag="PLM79_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86690.1"
                     /translation="MKPGQVHRVVAAALLALLAVLGRHETSSASETVVYSQSFEDSDG
                     GYTAEGNALWEWGTPSEVGPAKPNSGLKCWGTNISGNLPPGSEGAIISPPIAIPTLAR
                     GQVVRVRFFGWISIYDMTSRGEFMVSSDGVNWDSLAELFGNMSGEWQRYEFDISDKAG
                     GNLFLKFRAYEGDYSPDYPGFYVDDVAITVYEGSGAKKSFTLRAMEDTSPYASCPWVY
                     PWDGTDFVRDNDIYSVARLANREYTDFYLLQKDLVAQENVYRLEVREIESEVSFTDYA
                     GLVAVDHDENVAVGPDDRGNVFAYRPEELVVPQVAFSNSGADVTSKIGSPDNSGFHAY
                     ADDFVQVDFGGVDVSLGARLVLRVKGFVQGEGAWKPYTGPPAVVVKTLDEGGKWVERG
                     RLKPRFEWSECVFDLSPFLPDRNGEIKVRLESVSHKCKYHEIDFAGLSPGPEPVKAVR
                     NLPLLSADLGGEDISSLLNSSDNRYVTMSKGGKFSLSFAEEPQTLARRSFLFVSEGYY
                     IPQSSTFFIFTHDGRDWVMRDGFSLPYDPEDPYKLQTIVYDLSLFLPDPVGKYRVRVW
                     QDYRYETAGIDYVALEAGGKQGTLASAYYLPTQEDISYYVSAPDDDRVYSFYSGNRDR
                     WTEFEWTGLSANVPPTTDPVTYVDGTIRWTYRDADGDPQKAYQVAVWTGPGGTGEVVW
                     DPGAVSSSAGSVSYGGPPLKFQVTYYARVKAFDGKVWGQWYETAFSKGVGRVLEADVN
                     KDGRIGKLEDYHLIGVPGIIPANPDPLGVFGAGQESGYTPSRLRLFQYEPAGQDYVEY
                     PDLTPVAPGQGFWAITAIDRQLAVSGQFISDTTPYTVTVAPGWNMVGYPFLGRGALED
                     VVVRYREGGEWVEVPLGSQVDLKVEKEAWGYRSGYFAVRAGGAGLLDEWQAYWMNNVS
                     SENVQLLFYPSGTVPASPRTTASSVLPSLTQMPGGISFILSEKGRGYRDRTLFLGLDD
                     RASKGLDSLDCTSPPPVSKLAPRIYVNHGKWKVRPGQYAVDVRPPDALPEDFHVRLEL
                     PPRKGATRYVLRWKEIGTLPGNLKVSLEDPTNRRKVNMRKQSSYEFPVPAGKKRLAFI
                     VRMKS"
     gene            complement(9417..9563)
                     /locus_tag="PLM79_10035"
     CDS             complement(9417..9563)
                     /locus_tag="PLM79_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86691.1"
                     /translation="MFFLLLFNALFWGIPALIVLINIWSPVATELAILLLGLAVLARM
                     GKRV"
     gene            9576..9914
                     /locus_tag="PLM79_10040"
     CDS             9576..9914
                     /locus_tag="PLM79_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86692.1"
                     /translation="MNANTVEIVLKARDAASAVVSVAMSRVNRATRAYNGTLQQFNGQ
                     VKSSVQAFAGFAAQAVSVASVPRALPPGIRCVSRGQVSGWLNGMEPSGDLTPLRNEHR
                     HKKRVTVRSP"
     gene            complement(9931..10007)
                     /locus_tag="PLM79_10045"
     tRNA            complement(9931..10007)
                     /locus_tag="PLM79_10045"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9971..9973),aa:Trp,seq:cca)
     gene            10445..10708
                     /locus_tag="PLM79_10050"
     CDS             10445..10708
                     /locus_tag="PLM79_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86693.1"
                     /translation="MKIHYCKKYEKYVSRQHCEFFNRGKECDFYSPASWNSIKDLIQD
                     ENRPKWEVNAIIKPFQCDLLDREYLNRLNRRRRTRRVFLGNSL"
     gene            10888..12327
                     /locus_tag="PLM79_10055"
     CDS             10888..12327
                     /locus_tag="PLM79_10055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOV86694.1"
                     /translation="MFKKLLVANRGEIAVRIIRAAQELGIKTVAVYEETDKTSLHIMK
                     ADEAVCIGSGPRKDYLNIERMIQAALASGAEAIHPGYGFLAENPSFPMRCIEAGLVFV
                     GPPPQVISDMGSKVIARRIMTDAGIPVVPGTDVLPNNETGERSALEFAQEYGFPVMIK
                     AVAGGGGRGIRTARDPSELVKGLKTARSEAQMAFGNDRIYLEKGLLNPRHVEVQVLAD
                     HHGNVVHLGTRNCSIQRRHQKMIEIAPALLPQLMLDKICGMAVRAARASRYVNAGTVE
                     FLLDENDRFYFLEVNTRIQVEHTVTEMITGIDIVREQIRIAAGEPLSISQKDVTFRGT
                     AIELRITAEDPKNNFMAAPGVIQIYRTTGGHGVRLDGAVYQGYEIPRYYDSMLVKLTV
                     YGFTWREAVDRLDRALNGFTIAGVKTTIPFYRQIVKDPDFVEQRFDTSYIETHPQLLS
                     YQDDVPDMEKLAALIAEINAHGYNPYAEP"
     gene            12497..14155
                     /locus_tag="PLM79_10060"
     CDS             12497..14155
                     /locus_tag="PLM79_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HOV86695.1"
                     /translation="MSANDERITPKMTPREIREFLKGTPGYFMTNNERDVSQSDFKGR
                     ILPHTSLKVAPYRDETGYFALEISGGVTVHMDLMRKQINPFEKLRLVRRAMPRTLLQA
                     CVRARCLFGYRPYPDNVLRFAVRLFSRYIDVWRVYDCMNHVPNMEVVAEEVKNAGKLL
                     IASVCFNTGTGHTDDFYLRKVEEILQTLGEDLVVGIKNHSSVGSPKRIADLVGSIRKR
                     FPDLLLAYHGHNTDGNDLARMTSAVLEGVKIVEVADHGYGGIYSQAPALSLIQILNDY
                     GLRSPTLRIQPLVDASDVLRRERRLYDLFESPYRGFDPTVKRHKLTGGAASIAFEQAE
                     KLGLLERIHEVFGELIQVNRELGNLWSVTPASQILWTTAVNNVLHGRYEKPSDDLKRL
                     LLGRYGPFPFYEPQEWIFQKVLEHQRTDGKRWYQILADEAGIQRLENEDFDKRRAQLE
                     FELKRPARDEELCLYLMFPRDALEYFRFEEQYGRPWLLPPEVWYRRGGFPDGARVTFS
                     DYEGKTHHIDIVSTRRSGDDVHTSLLVDYHFQTYTSTVRSKEAG"
     gene            14161..15054
                     /gene="xerD"
                     /locus_tag="PLM79_10065"
     CDS             14161..15054
                     /gene="xerD"
                     /locus_tag="PLM79_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOV86696.1"
                     /translation="MEGGLDLFLVFLSVERGLSANTITAYGHDLVEVCRYLQDRGIRG
                     WDEVTRQHLTDYLEYMGSSLSRRSAVRRLAAVKSFFKYLESRGEIPGNPLARVPLPRL
                     KPTLPRALSFREVESLLAGPQPSALPYGQRDKAVLELMYATGMRVSEVTDLEMRQLHL
                     DAGYLVVRGKGDKERLVPMGEYAAQALKAYLLQGRSLLLKHRKGFAPEVFLNHRGRRL
                     SRQSVWKMVKQYAIRAGIRANLTPHTLRHSFATHLLENGADLRSLQAMLGHKDISTTQ
                     IYTHVARARLKEIHAKHHPRP"
     gene            15064..17733
                     /locus_tag="PLM79_10070"
     CDS             15064..17733
                     /locus_tag="PLM79_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV86697.1"
                     /translation="MEVITTHINADFDAMASMIAAKKLYPGALLVFPGSQERTLREFF
                     VKSTVYLYDFKRLRDIDLQEVRRLILVDTRQLSRIGKFEEVLGKKDLEIHIYDHHPPS
                     EDDIEGHVSVIRPVGATVTILTHLIREREISVTAEEATIMSLGIFEDTGSFTFNSTTP
                     EDFEAAAYLRRCGADLNVVADMVTQELTAEQVNLLNDLLLSARTFNIQGIEVCVTTVS
                     VDRYVADFAVLVHKLKDIVNLDVIFALARMEDRIYLVARSRIPEVNVGKIASYFGGGG
                     HSNAASASIREMTLNQAEDRLLEVLKSSVRPFPSAENLMTSPVIYSEGERSIGDAAQI
                     MVRYNINAMPVMDDGQLVGVINRQVLEKAIYHGLEHRKVREFMNSDFSTVEPTATLME
                     IQTHLVEQQQRILPVVDHGKLLGVITRRDLLNFLVSDHTVTPRALYDDLNEAHWPKRK
                     NILGVIREQLPPEVIVILQDLGKLAEELHYKAYAVGGFVRDLLMRRPNLDIDVVVEGD
                     GIEFARAFGERHDVRVRSHKKFNTAVLIFPGGQRVDIATARFEYYQYPAALPIVEFGS
                     LKMDLYRRDFTVNTLAVDLNPRTFAQLIDFFGGQRDLKEKMIRVLHNLSLVEDPTRIL
                     RAIRFEQRFGFRIAKQTANLIRTAVQMGLIQKLGGHRLFHELQLILMEDNPLPALRRM
                     GEYGVLAVLSRKMKFDTKMEELVTRINEVTSWHRLSFLDEPLEKWWVHFLGLLSALTP
                     TELEKAFGRLELNSAQKERILWTYAQVRDLLGGFFLLPDPRPSDIYRALQPFRPEELL
                     FMMAKTQREEVRKAISHYFHRYRNVRTEVKGRDLRALGIPPGPVYRKVLDDLLTVRLN
                     GEVRNRQEEIEYVERHYRELVPRKGLDSSSVSN"
     gene            17938..>18516
                     /locus_tag="PLM79_10075"
     CDS             17938..>18516
                     /locus_tag="PLM79_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOV86698.1"
                     /translation="MWQETILNALLYSVPLLLAVVAHEVAHGLAAEKRGDPTARMQGR
                     ITMNPLVHVDLVGTILLPGILIATRSPFLFGWAKPVPVDLTNLKGGRRDMALVSAAGP
                     LSNLVLACLSALTYRGVILAINALGSPPELLWVLEPLMIMAAFSATFNLTLMVINLLP
                     IPPLDGGRILMGLLPPRLAVQLQKVEPYGVLIL"
BASE COUNT         3805 a         5797 c         5389 g         3525 t
ORIGIN      
        1 caagccccgg tttatcagcg ggacccgcgc ctttgtcccc aacggcctcc tttgccggga
       61 cggccccggg gctcccggcc cggccatcga tgcagggtcc gcctcgccga gcccccagcg
      121 acacggagag agctgcaaga tggaaaccct gaagagttat atcgacgaga aacagcaggc
      181 cttcatcgtg tgttcccaat gcggggcgcg caggatcgtc gacgcctcca agatgggaag
      241 cctcagcgaa atcaaggcga aatgcacctg cggtttcgtc ttcctagtgt cctttgaacg
      301 ccggtacttc taccgaaaga aggtcaatct gcgaggggag tacctacgcc ccccgccgga
      361 taagtcctgg ggcgaagtgc tggtcgtcga catctcccga acgggtctgg ccttccgtcg
      421 ggtgtccggc agccccctga agacgggaga gaccgtgaag atcaccttca ccctggacga
      481 cccggcccag tccgtcatca gcaagaacgt catcgtgcgg cgggtggatg gcgacaagat
      541 cggagcggaa tttgtggatc gaaacctggg caaggccctc gggttctacc tgatgccctg
      601 aaaagacgca ggcggaccga cctccctttc gacatcgacg ccatggaaac actctatcga
      661 ttcaggaaaa tcgaccccca agcctacgac gacctgcgcc tacaggtcag cagggagtac
      721 acccaggtga agatgcgcga tttcttcctg ggaaagccgg ttcccgtcga gatttatttg
      781 ccgatgatgg gaaggtccga aaccctctcc atcgagaagg tggtccacaa ggaccaggtc
      841 tacgagcctt ccctccatcg acgcctggcc gaggaaggaa tccacaccgc cttcatccgg
      901 cacgaggacg agaccgcgtt tctcaactat ttcagcgagc aggtgcgaga gactctccat
      961 tcccaggacc ttccttcgga gaaaaagacc cagctccttt acgacaacgc ggaaattctc
     1021 atcaagaagg tgttccggga acgtcccacc tttgccggcg tttccatggg gcgaaacctg
     1081 gtcgagagct ttgccgtcca cctgtccgtc gactccgttc cctcccaggc gctcctttca
     1141 attttttcca aggattatta cacctttacc cattcgatcc aggttgccat cctgggcatg
     1201 tccttctgcc ggttcctggg gtggagcaag gaagaaaccg tggatttcgg actgggggga
     1261 cttttccacg acatcggcaa gagtgccgtc gacgacaaga tcctgaacaa accgggaaag
     1321 ctggacaaag acgaattcga gctcatcaag cgccactgtg ttctgggggt cgaacagctc
     1381 aaggacgccc acgccatgtc cagggagcag ctgagggtgg tattgcacca tcacgagggc
     1441 atggacggat ccggttatcc cgacgggatc gagggcccgg agattcaccg gtacgcccgc
     1501 ctggctcgaa tcgtggactg cttcgacgcc ctcaccacca agaggcctta caaagacgcc
     1561 atgccgccgg cggaggcctt ggagctcatg cggcgggaga tgagccgggt actggacccc
     1621 caccttttca aggcgtttgc aagctttctg cacctggaag acccggttcc cgaggtcttc
     1681 caatcccagc gcatgaacat cgagttgggc acccgggtgt tcatggaggg gaccggcggc
     1741 gtgcaggtga agacttccct ggtgggcatg gacccggatc gctacctcat cgtgaagccc
     1801 cccaagggcg ccggtgccgt ccaaccgttt tgcctggggg tcgatatcat ttgccgctat
     1861 ttccactcgg gctacgtgta cggctttcgg tcccgaatcc ttcaccggac cttcacgccg
     1921 tttgcgctcc tcttcctttc ctacccgaag gccgtcgaga ccgtcaacct ccggaaggaa
     1981 cccaggatcg actgttttct tgcttccgag gccgtcattg ccggggtgaa gcacccgggc
     2041 gtcatcgccg acctgagcac cggcggctgc cggttcgtgg tgggacagga tcgagacctg
     2101 ggactgcccc aggtgaaggt ggaagatgag cttatcatcc acacgaggtt tgcgggagac
     2161 gccgagccca aagccctccc gggaagggtc cgcaacctcc ggatggaaaa ggagaaggtg
     2221 atcctcgggg tcgagttcga aaacctcggc ccggagactg tgcttcatct ggaaaaatgc
     2281 gtgcgggagg tcctggacct gatcctgtga tgcatgggcg ggtcagggcc ctccgggact
     2341 ttcccgtgcc cgagcgcttc accgtcctcg ggtccctccc gtcgaggaag tcggcaaacc
     2401 cttgccgacg tccgggaaaa ggcggaaagc cgcctctgcc ccgtgcggag agggacgggg
     2461 agaatggaag ccccattgcg gatgagcccc ggacggagtc ccttgatgct catccgggaa
     2521 agcgccgccg aaacctttcg aacctctgtg cgggagatct ttcatgaccc ttcccggccg
     2581 gaagctgact ccgacgagtt ccatagagag tgtggactat tcccttcttc gagaatacct
     2641 ggattcccga gcccaccggg ccgatgactt cctccagaaa agtctgctgg gcgaactcat
     2701 cggcaagtat gtcgacctgg aaagacgggt ggatgcgctc ctcaagaaca ccctcccggc
     2761 caccgtcgcc gaagagatca agcactccgg aaccttcagc ccccgatcct acgactgctc
     2821 catcctgttt tcggacatcg caggcttcac ccgactggcg gaaagagtcc ccggcgaggt
     2881 cctcctccag atgatcgatg cccttttccg cggtttcgac gaaatcatgg aacggttcgg
     2941 gggcaccaag atcaagacca tcggggatgc ctacatggcg gttttcgggg cccccgagcc
     3001 cctcgagaac catgcggtcc aggcagtgaa gacggccctg gccttccagg actttgtgca
     3061 acggttccgc ggggcacggg acctggacct tggcctgcgc atcggggtcc attccgggtc
     3121 ggtgatggcg gcggtggtgg gaaaggagcg gatgcagttc gatgtgttcg gagacaacgt
     3181 gaacgtcgct tcccggatgg agagcgcagg agaaatcgac cgggtcaacg tctctcacga
     3241 agtctacctc agggccgcag acgccttcat cttccgggaa agaggcgaaa tccccctgaa
     3301 aaaccgtggg ctcatgaggg cctattttgt ggagggagaa cgaaaagatg tgccccagtg
     3361 acaagaatct ctacgatcgg ccggtgctca tagccccggg cgtctattgg gtgggttacc
     3421 aggacacgat ctataacatg cactcgaacc cttacctcgt ggtggaaggg gacgaagccg
     3481 tgctcattga cgggggaagt cgccccgact tccccgtagt catgaccaag gtcctgcaga
     3541 cggggctgcg aatcgaacag atccgggcaa tcatcctgca ccactacgac cccgacctgt
     3601 gcgggtccct gcccaacatc ctggatctgc tgggcaagaa aagtgttcaa gtgttgtccg
     3661 cggaagccaa ccacatgttt atcgcctact atctggccaa ggagcatcac cccctgctga
     3721 cccccaccga ggcctacccc gaaggctacg atctcaacgg caggaccctg cgcttttacc
     3781 cgacacccta cgcccatgct ccggggagct tcatcaccta cgacacccgg accaagaccc
     3841 ttttcaccag cgacctgttc ggtagcctgt ccaggaaatg ggacctgtac gccgagctgg
     3901 ccccggaatg cgaacaatgc cccgactacg agcactgtcc caacgggcgg gattactgcc
     3961 cgctccccga tatcctggac ttccatcggc aggtgatgcc caccgcgcgg gccgtaagga
     4021 tggccatgga gacgatccgg ggcctggacg tggaaatcct cgcaccacag catgggagcg
     4081 tgttcgctcg gcggagcgac atcgattttc tctgtcgcat gctggcgggc atggatcgca
     4141 tgggcatcga cgagagggcg ggggggtgat gcccaacccc ggacctccgt cttcgacaag
     4201 ttccggcggg gtcactcaag ggcgaccccg gatttgttgt tccagggact cctggtggga
     4261 cctggtgtcc gtcaggatct tgtgcagggc tttctggtat tcgggatggt tcgttttgac
     4321 gatggcggcg tattgggact cgaggatgga gcgctcgagg tcccgggcca ggaacagggc
     4381 ttttttctgg gtaagctcct ggttcttcag ctttttcagg ttgtcgcgca ccccttgaat
     4441 cacggtccgc acggccacgg gattcatggg acgccccacc tgaaaatgag acggatcgga
     4501 tttcaggagc gctttcatct cctggaggaa ttgggcgtgg agcctttcgg cggccgccag
     4561 gtcccgccac aaggcctcat cctcccccag gatctccgcg cagctctcgt aaaaaaccgc
     4621 catttcctgc tccaacaaag ccatcgctcc catcagttct tccactttgc gcgcttctcc
     4681 gggcagcata gatcccctct tcatcgcaaa agggccgatg cacactctcc ccccatcccc
     4741 cttcccggac ggggatcacg aaagactgca aagccggtga acggttttgc tgcaaaccca
     4801 ttcaccggct catcgtcttt cctgcgaatc tccaagctca ggacttcatc cgcacaatga
     4861 aagcaagccg cttcttcccg gcaggcaccg ggaactcgta actcgactgt ttgcgcatgt
     4921 tcactttccg ccgattggtt gggtcctcga gggacacctt gaggttgccg gggagtgtcc
     4981 cgatctcctt ccaccgcagc acgtaacgag tcgcaccctt tcgaggcggg agctccagcc
     5041 tcacatgaaa gtcctccgga agggcgtccg ggggcctcac atccaccgca tattggccgg
     5101 gcctcacctt ccacttgccg tgattcacgt agattcgagg agccagtttg gaaacggggg
     5161 gaggagaggt acaatccagt gaatcgagtc ccttggaagc ccgatcatcc aatcctagga
     5221 agagggttcg atcccgatat ccccggccct tttcgctcag gatgaaggaa atgcccccgg
     5281 gcatttgggt gagggaagga aggacggacg acgccgtggt ccgcggtgag gcgggcacag
     5341 taccggaagg gtaaaaaagc agctgtacgt tttcgctgct gacgttgttc atccagtaag
     5401 cctgccactc gtcgagcagt ccggctcccc ccgcccgaac ggcgaagtat cccgatcgat
     5461 atccccatgc ctctttttcc accttgagat ccacctggga cccgaggggg acttcgaccc
     5521 attcccctcc ctcccggtag cgaacgacca catcctccaa ggctccccgg ccaagaaacg
     5581 ggtatccgac catgttccat cccggagcca ccgtaaccgt gtagggagtg gtgtcggaaa
     5641 tgaactgtcc ggacaccgcg agctgccgat caatggcggt aatggcccag aatccctgtc
     5701 ccggggcgac gggcgtcagg tccgggtatt ccacataatc ctgcccggcc ggctcatatt
     5761 ggaacagacg caaccgtgaa ggagtgtacc cggactcctg gcctgcgcca aacaccccga
     5821 gggggtcagg attggcggga ataatccccg ggacgccgat caggtggtag tcttccagct
     5881 tgccgattct gccgtccttg ttgacgtcgg cctccaagac gcggcccact ccctttgaga
     5941 aagccgtctc gtaccactgg ccccaaacct tgccgtcaaa cgccttcacg cgggcataat
     6001 acgtgacttg gaatttgaga ggaggcccgc cgtaggaaac ggaaccggca gaagaactga
     6061 cggcaccggg gtcccacacc acttcccccg tgcctccggg accggtccag accgccacct
     6121 gataggcttt ctgaggatcg ccgtccgcat cccgataggt ccaacggata gtcccgtcaa
     6181 cgtaagtaac cgggtccgtc gttgggggca cgttggcgga gagtcccgtc cattcgaatt
     6241 cggtccaccg gtccctgttt ccggaataga agctgtagac tcggtcgtcg tcgggggcac
     6301 tcacgtagta ggagatgtcc tcctgtgtcg gaagatagta ggccgacgcc agggtcccct
     6361 gcttcccccc tgcctccaga gcgacatagt caatcccggc ggtctcatat cgatagtctt
     6421 gccaaacccg gacacggtat ttgccgacgg gatcgggcaa gaacaaggac aggtcgtata
     6481 ctatggtctg caacttatag gggtcctcgg gatcgtacgg aagggaaaaa ccgtcccgca
     6541 tgacccaatc gcgcccgtcg tgggtgaaga tgaaaaaggt gctgctttgg ggaatgtaat
     6601 acccctcgct tacgaaaagg aagcttctcc gagccagggt ttgaggctcc tcggcaaagg
     6661 aaagcgaaaa cttccctcct ttgctcatgg tcacatagcg gttgtcggag gaattcagca
     6721 gggacgagat atcttccccg cccagatccg cgctgagaag gggcaggttc cgcaccgcct
     6781 tcacgggttc cggacccggg gacaaccctg caaagtcgat ctcgtggtac ttgcacttat
     6841 gggagacgga ctcgagtcgg accttgattt ccccgtttct atccggaaga aacggggaca
     6901 agtcaaacac gcattcggac cattcgaacc ttggcttcag gcggccccgt tcgacccatt
     6961 tgcccccttc atcgagggtc ttcaccacca ctgcgggagg gccggtgtaa ggcttccagg
     7021 cgccttctcc ctggacgaag cctttgaccc gcaggaccag gcgagccccc aggctcacgt
     7081 ccacgcctcc aaaatccacc tgcacaaagt cgtcggcgta ggcatggaaa ccggaattgt
     7141 ccgggcttcc gatctttgac gtgacatccg ccccggaatt gctgaaggcg acctggggaa
     7201 cgaccagttc ctcgggtcgg taggcaaaca cgttgccgcg atcgtcgggc ccgacggcca
     7261 cattctcgtc atggtccacg gccaccaatc ccgcatagtc cgtgaaggac acttccgact
     7321 cgatctctcg gacttccagc cggtagacgt tctcctgggc aaccaagtcc ttctgcagaa
     7381 ggtagaaatc cgtgtattca cggtttgcaa ggcgggccac cgagtagatg tcgttgtctc
     7441 gaacgaagtc ggtcccgtcc catggataga cccaggggca ggaggcgtag ggagaagtgt
     7501 cttccatggc cctcagggtg aagcttttct tggctccgga gccttcatag acggtaatgg
     7561 caacgtcgtc gacgtagaag cccggatagt ccggggaata atccccttca taggcacgga
     7621 acttaagaaa caggtttccc ccggccttat cggaaatgtc gaattcgtaa cgctgccatt
     7681 caccgctcat gtttccgaag agttcggcca gggaatccca gttgaccccg tccgaagaca
     7741 ccataaactc gcccctgctc gtcatgtcgt agatgctgat ccacccgaaa aagcgcaccc
     7801 ggacgacctg gccccgggcc aacgtcggga tggcaatagg cggcgaaatt atggctccct
     7861 cacttccggg gggcagattg ccggaaatat tcgtccccca gcacttcaat ccggaattgg
     7921 gtttagccgg cccgacctcc gaaggagttc cccactccca cagggcattg ccctccgccg
     7981 tgtaaccgcc gtcgctgtct tcgaaacttt gggaataaac gacggtctcg ctggccgaag
     8041 acgtttcgtg ccggcccagg acggccaaca gcgccaacaa ggccgccgcg acgactcggt
     8101 gaacctgtcc gggcttcata ggattctcct cctccccgtc ataggggccc taaaccacgc
     8161 ctcaactctg ctgaatgacg gtatccatgt gtggacccat tcatgcagtg gcgcccatcc
     8221 atgccttcgg ctccgtgcct tgatgaggca acccccctcc aaaccccacc gggaaacggt
     8281 ttgccgtgat cgcgttttgg aaatcgtcga tttatgctgc cggacggatt tgccgcccgg
     8341 agccaaatag aaatacgctt cgagaccgct ctttcacctg aacggacacc ccgtgcattg
     8401 gggaattctc ccgtgaatag cccgtgggga ctgggtgatc gtctgcagga agaagaattc
     8461 agtgtcgtct ttcctggaac ggcaagagaa tgagatacgc gattcatttt ccgtcgggag
     8521 taggcgaaag tcaataagat ctctgaccgg ttgtgcaaaa accccccaaa acccgggcat
     8581 ggaacggacc tcgggcgtag aagcacgttt gccggtctgt gaggtgcggc gctgggggct
     8641 ctccgggcgc ctcgtcaaac gatgaatcga tggaatttgc cttggaagta cgatcccttg
     8701 cggcaaaggg gaggaagtcc gcaaaggatc acggggtccc cagccggacg ccttcatgga
     8761 acgtctccgg gaaggaccga gctcccgcag gtagcgggac ggattctcga ggggccgacg
     8821 gcgccacaag ggacggccgt acggacccgt gtggcccgat gctcttccgg ccgcaccaca
     8881 cctgtcccca tgagggtgca tttcactccc ttgttcggaa cgtgtgggtg aatcttcctt
     8941 ccgttgcacc gtgcctgtgc cgatgaggat aaattccact tcttgatgct tcaaggaggc
     9001 cgggcgttga taccccggaa aacgagatat tcccaaaatc atcctcatcc aggtatttcc
     9061 cgaagtgccc actcactctt agcgattgtc tctgtgcctt tagaaccttc cacatttcgt
     9121 ccgaatacgc cacggaaccc accgagagat tgactcttgt gaccaggaca acctcgtcgg
     9181 cttccgagat gccggcggcg cgcaggaagg agcgaaaccg ctccacatag acctggaaga
     9241 aggtcgtagg gtatccccct gcccaacggc ctgggcctcg tcctagcgga ccccgaacat
     9301 gcccatggca cacagggcaa tcagcaatgc cgggacccga acatcgaaca ggatgaacat
     9361 ggaaacctcc ggcagaggac tggttggtgg gtttgggcga ccggccggtc gcccccctat
     9421 acccgttttc ccatccgggc gagcacggca aggcccagga gcaggatcgc gagttcggtc
     9481 gcgacgggac tccaaatgtt tatcaggacg atcaatgccg ggatgcccca gaacagggca
     9541 ttgaatagca ggagaaaaaa catggagacc tcctcgtgaa tgccaatacg gtggaaatcg
     9601 tcctgaaggc ccgggacgcc gcatctgccg tcgtcagcgt agccatgtcc cgcgtgaacc
     9661 gggcgacccg cgcctataac ggcaccttgc agcagttcaa cggccaggtc aagagctccg
     9721 tgcaagcctt tgcgggattt gccgcccagg ccgtctccgt ggcgtccgtc ccaagggcgt
     9781 tgccgccggg catccgttgc gtgagcaggg gtcaggtctc cggatggctc aatggcatgg
     9841 agccatccgg agacttgacc cccctgcgaa acgagcacag acataagaaa agggttacgg
     9901 tccgttcccc gtaaccctta tttttctcct tggcaggcca ggagggattc gaacccccat
     9961 ccctcggatt tggagtccga tgctctaacc gttagagcta ctggcctgcg ggcggaatcc
    10021 tatatagcgg tttccaccgg ggatgtaaag ggaaaacatg atgttgtttt cacatagaaa
    10081 tcgggggagt agaacgatct tccgggacgg cggccccggg attttcggcg ggtgtccggg
    10141 tctgccaggc ccgggttgag gggttcccgg gggcgacgac agcgggaccc caaccccatc
    10201 ggcacaccgg gagaccaggt ctcttgcccg aaaccaaggc cgtagactta cggaaatctc
    10261 acacaagaaa ggccggccgg cccggaggta ctcttccctc aaaaaatagg acaaacttcc
    10321 gatattccct tggaaatcaa tggatttgat cttgtcaaac cccggcctcc cgggtacagt
    10381 accgtacaga tgtccatcat catcccttgg tcatgcgata ggacctctga aaaaggaggg
    10441 atcgatgaag atacactact gcaaaaagta cgagaaatat gtcagccggc agcactgcga
    10501 attcttcaat cggggcaagg agtgcgattt ctacagtccc gccagctgga acagcatcaa
    10561 ggacctcatc caggacgaga atcgacccaa gtgggaggtg aacgccatca tcaaaccttt
    10621 ccagtgcgat ctgctggacc gtgaatactt gaaccggctg aatcgaaggc gacgaacccg
    10681 aagggtgttc ctgggcaatt ccctgtaatt ccttcttcgg gagcgccgtt cccgtcttgc
    10741 ccggcggcct ccccttgtcc cccgtccgtt cccatcatct ccgccattga agcttcccgc
    10801 ccacttgtgc taagagttcg gagtaccccg tatccggcat cggcagcaaa ggtccgagaa
    10861 caaatctatt caaaaacgtg aggcgttatg ttcaagaagc tactggtggc aaaccgaggg
    10921 gagattgccg tccgcatcat acgtgcggcc caggaactcg gaatcaagac cgtggccgtc
    10981 tacgaggaga cggataagac ctctctgcac atcatgaaag cggacgaagc cgtctgcatc
    11041 ggctcggggc cccgaaagga ctacctgaac atcgagcgca tgatccaggc agccctggca
    11101 tccggcgccg aagccattca tcccggctat ggatttcttg cggagaatcc cagttttccc
    11161 atgcggtgca tcgaagccgg gctggtgttc gtgggacccc ctccccaggt catcagcgac
    11221 atgggcagca aggtcattgc ccggcggatc atgaccgatg cggggatccc cgtcgttccc
    11281 ggcacggacg tccttcccaa caacgagacg ggggaacggt cggccctcga gtttgcccaa
    11341 gagtacggct ttcccgtcat gatcaaggcc gtggccggag gcggaggacg gggcattcgc
    11401 acggccaggg acccgtccga actggtcaag gggctcaaaa cggcccggtc cgaggcccag
    11461 atggctttcg gcaacgaccg gatctacctg gaaaagggac tgctcaatcc acgccacgtg
    11521 gaagtccagg tcctggcgga tcatcacggg aacgtggtcc atctgggaac ccggaactgc
    11581 tccatccaac gccgtcacca gaagatgatc gaaattgcac ccgccctgct tccccagctt
    11641 atgctggaca agatttgcgg catggccgtc cgggcggccc gcgcctcccg ctacgtcaac
    11701 gcgggaaccg tcgagttcct cctggacgag aacgaccgat tttacttcct ggaggtgaac
    11761 actcgaatcc aggtggaaca cacggtgacg gagatgatca cgggaatcga cattgtgcgc
    11821 gagcagatcc gcatcgcggc aggagagccc ctgtccattt cccagaaaga cgtcaccttt
    11881 cgaggtacgg ccatcgaact gcgaatcacc gccgaggacc ccaaaaacaa cttcatggcc
    11941 gctccggggg tcatccagat ctaccgaacc acggggggcc acggggtccg gctggacgga
    12001 gccgtctacc agggatatga gatcccccga tattacgatt ccatgctggt gaagctgact
    12061 gtttacgggt tcacctggag ggaagcggtg gaccggctcg atcgggccct caacgggttc
    12121 accatcgccg gggtcaagac gaccatcccc ttttatcgcc agatcgtcaa ggacccggat
    12181 ttcgtcgaac agcggttcga cacctcctat atcgagaccc atccgcaact gctgtcctac
    12241 caggacgacg tccccgacat ggagaagctg gcggccctca ttgcggagat caacgcccac
    12301 ggatataatc cgtacgcgga accatgacgg tccccttttc cccgggtctt gcgcaacgga
    12361 tggcggaagg gccccgggca aagaagtccc tcggtccgac ccgggcggcg tcggaacggg
    12421 gaaggtcccg gacggttttt ccgtttgcgc tgcgcataag cggcgaaacg agcctattgg
    12481 aagggagttc aaccctatgt cggccaatga cgaacgcatc accccaaaga tgacccctcg
    12541 ggagatccgg gagtttctga agggcactcc gggctacttc atgaccaaca acgagcggga
    12601 cgtttcccag tcggatttca agggccgcat ccttcctcat accagcctga aggtggcacc
    12661 ttaccgggac gagacgggat atttcgccct cgagatctct gggggcgtta ccgttcacat
    12721 ggacctcatg cgcaaacaga tcaacccctt cgagaagctg cgcctggttc gcagagccat
    12781 gccgcgaacc cttctccagg cttgtgttcg ggcgcgatgt cttttcggct accgtcccta
    12841 tccggacaac gtcctgcggt tcgccgtccg gctgttttcc cggtacatcg acgtctggcg
    12901 ggtctacgac tgcatgaacc acgtaccgaa catggaagtg gtggccgagg aggtgaaaaa
    12961 cgccgggaag ctgctcatcg cctccgtctg cttcaacacg gggacgggtc acacggacga
    13021 tttctacttg cgcaaggtcg aggaaatcct ccagaccctg ggagaagacc tggtcgtggg
    13081 gatcaagaac cactcgtccg tcggctcgcc caagcgcatc gccgacctgg tgggctccat
    13141 ccgaaaacgc tttcccgacc tgctcctggc ctatcacggt cacaataccg acggcaacga
    13201 cctggcgaga atgacctcgg cggtgctcga aggggtgaag atcgtggaag tcgccgacca
    13261 cggttacggg ggcatctaca gccaggcccc ggccctgagc cttatccaga tcctgaacga
    13321 ctacgggttg cgctccccca ccctccggat ccagcccctg gtggatgcct ccgacgtcct
    13381 ccgccgggaa aggcgtctct acgatctttt cgagagcccc taccgggggt tcgaccccac
    13441 ggtcaagcgt cacaagctca cgggcggagc ggccagcatc gccttcgaac aggcggaaaa
    13501 actggggctt ctcgagcgga ttcacgaggt gttcggggag ctcatccagg tgaaccggga
    13561 actgggcaac ctctggtcgg tcactcccgc cagccagatc ctctggacca ctgcggtaaa
    13621 caacgtcctc cacgggcgct atgaaaaacc ttcggacgac ctcaaacgcc tgctcctggg
    13681 ccgctacggg ccctttccct tctacgagcc ccaagagtgg atcttccaga aggtcctgga
    13741 acaccagcgc acggacggaa agagatggta ccagatcctg gcggacgaag ccgggattca
    13801 gcgtctcgag aacgaggact tcgacaagcg ccgggcccag ctcgagttcg agctcaagcg
    13861 gccggcaagg gacgaggaac tctgcctgta tctcatgttc ccccgggatg ccctcgagta
    13921 cttccggttc gaggaacagt acggcagacc gtggctcctg ccccccgagg tctggtaccg
    13981 gcgaggaggt ttccccgacg gagctcgagt gaccttttcc gactacgagg gcaagaccca
    14041 tcacatcgac atcgtttcca cccggcgctc gggggacgac gtccacacgt ccctcctcgt
    14101 ggattaccat ttccagacgt acacctcgac ggtccgatcc aaggaagccg gttgagatcc
    14161 atggaaggcg gactcgatct cttcctggtg ttcctgtcgg tggaaagggg attgagcgcc
    14221 aacaccatca ccgcttatgg ccatgatctc gtcgaggtgt gtcgatatct ccaagaccgg
    14281 ggcattcggg gatgggacga ggtgacccgg cagcacctga cggactatct tgaatatatg
    14341 ggatcgagcc tgtcccggcg cagtgcggtt cgccgtcttg cagccgtcaa gtcctttttc
    14401 aagtacctgg aaagccgagg ggagatcccc ggaaaccctc tggcccgggt gcccctgccc
    14461 cggctcaaac cgaccctgcc ccgggccctt tccttccggg aggtggagtc cctcctggcc
    14521 gggcctcagc cgtccgcgct cccctacggt cagagagaca aggctgtcct ggaattgatg
    14581 tatgccacgg gaatgcgggt cagcgaggtc accgatctcg agatgcggca gctccacctg
    14641 gatgccgggt acctggtggt tcgcggcaag ggagacaagg agcgcctggt gcccatgggg
    14701 gaatacgccg cccaggccct caaggcctac ctgttgcagg gacgctccct gcttctcaag
    14761 caccggaagg gattcgctcc ggaggtcttc ctcaaccacc ggggccgacg gctttcccgg
    14821 cagtccgtct ggaagatggt caaacagtac gccatccggg cgggaattcg ggcaaacctg
    14881 accccccaca cccttcggca ttccttcgcg acccatcttc tcgagaacgg agccgacctt
    14941 cggtccctgc aggccatgct gggacacaag gacatctcca ccacccaaat ctacacccac
    15001 gtggcccggg cccgactgaa ggagatccat gcaaagcatc acccgcgtcc ctgaggtacc
    15061 tccatggaag tgatcacgac tcacatcaat gcggacttcg atgccatggc ttccatgatc
    15121 gcagccaaga aactctaccc cggcgccctc ctggtctttc cgggttccca ggagcggact
    15181 ctccgggagt ttttcgtcaa gtcaacggta tacctctatg atttcaaacg cttgcgagat
    15241 atcgacctcc aggaggtccg ccgcctcatc ctggtggaca cccgtcagct gtcccgcatc
    15301 ggcaagttcg aagaagtcct gggaaagaag gacctcgaga tccacatcta cgatcatcat
    15361 cccccgagcg aggacgacat cgagggtcat gtttccgtga tccgtcccgt gggcgccacg
    15421 gtcaccatcc tcacccacct catccgggag cgggagattt ccgtcacggc ggaagaagcc
    15481 accatcatga gcctggggat tttcgaggac acgggatcgt tcaccttcaa ctcgaccacc
    15541 cccgaggact tcgaagccgc ggcctacttg cgccggtgcg gcgccgacct gaacgtggtg
    15601 gcggacatgg tcacccagga actcacggcc gaacaggtga acctgctcaa cgacctgctt
    15661 ctttcggccc ggaccttcaa catccagggg atcgaggttt gcgtgaccac ggtttccgtg
    15721 gacaggtacg tggcggattt cgccgttctc gttcacaaac tcaaggacat cgtcaacctc
    15781 gacgtcatct tcgccctggc ccgcatggag gaccggattt acctggtggc ccgcagccgc
    15841 atacccgaag tcaacgtggg aaagatcgcg agctatttcg gaggaggcgg acactcgaat
    15901 gcggcctcgg ccagcattcg cgagatgacc ttgaaccagg cggaggaccg cctcctcgag
    15961 gtgctcaaga gctccgtgcg cccgtttccg tcggcggaga acctcatgac cagcccggtc
    16021 atctattcgg aaggagaacg ctccatcggg gatgcggccc agatcatggt ccgctacaac
    16081 atcaacgcca tgcccgtcat ggacgacggg cagctcgtgg gagtcatcaa ccggcaggtc
    16141 ctcgagaaag ccatctacca cgggctcgaa caccgaaagg tccgggaatt catgaactcg
    16201 gacttctcga cggtggagcc cacggccacc ttgatggaaa tccagaccca cctggtggaa
    16261 cagcagcaga gaattctgcc cgtggtggac cacgggaagc ttttgggggt catcacccgc
    16321 cgggaccttc tgaatttcct cgtctcggac catacggtga cgcccagggc cctctacgac
    16381 gacctgaacg aagcccactg gcccaagcgc aagaacatcc tgggcgtcat ccgggaacag
    16441 ctccccccgg aggtgatcgt catcctccag gacctgggga aactggccga ggagcttcat
    16501 tacaaggcct atgccgtggg cggctttgtt cgggaccttc tcatgcggcg gcccaacctg
    16561 gacatcgacg tggtggtgga gggcgacggg atcgagtttg cccgggcctt cggggaacgc
    16621 cacgacgtgc gcgtccgttc ccacaagaaa ttcaacacgg cggtcctcat ctttcccggg
    16681 gggcagcggg tggacatcgc cacggcccgt ttcgaatact accagtaccc ggccgccctt
    16741 cccatcgtgg aattcggctc cctcaagatg gacctgtacc gccgggattt caccgtgaac
    16801 actctggcgg tggatttgaa ccccaggacc ttcgcccagc tgatcgattt cttcgggggc
    16861 caacgggacc ttaaggagaa gatgatccgg gtcctgcaca acctcagcct cgtggaggac
    16921 cccacgcgga tccttcgggc cattcgcttc gagcagcggt ttgggtttcg catcgccaag
    16981 cagaccgcca acctgatccg cacggccgtc cagatggggc tcattcaaaa actgggggga
    17041 catcgcctct tccacgaact gcagctgatc ctcatggagg acaaccccct gcctgccttg
    17101 cgcagaatgg gcgagtacgg ggtcctggcg gtcctttccc ggaagatgaa gttcgacacc
    17161 aagatggagg agctggtcac ccgcatcaac gaggtcactt cctggcatcg cctcagtttc
    17221 ctggacgaac ctctcgagaa atggtgggtc cacttcctgg ggctcctgtc cgccctcacc
    17281 cctacggaac tggagaaggc cttcgggcga ctcgagctca acagcgctca aaaggaaaga
    17341 atcctctgga cttatgccca ggttcgggac cttctcgggg ggtttttcct gttgcctgat
    17401 cctcgaccca gtgatatcta tcgtgcactt cagccgttcc ggcccgaaga gctcctgttc
    17461 atgatggcaa agacccagcg ggaagaagtc cgcaaggcga tcagccacta ctttcatcgc
    17521 taccggaacg tgcgaacgga agtgaaaggc cgggacctga gggctctggg cattcctccg
    17581 gggcccgttt accgcaaggt cctggacgac ctgctcacgg tgcgtctgaa cggagaggtc
    17641 cgaaaccggc aggaagagat cgaatacgtg gagcggcatt accgggaact cgttcctcgg
    17701 aagggcctcg attcttcctc cgtatcgaat tgaaggaggg tgctccggtc cggctccatg
    17761 gaaacgggct tcgagcatgg atccggagtc ggtctcgatc ccggccgacc ccggtcccga
    17821 cggggcgccg aaatctcccg ggtgggttcg gaggtcggtt tggacctcga gcatcccggc
    17881 aactggggac cccagacggt gcggagcggt tcatgcaatg caccccggcg gaccttcatg
    17941 tggcaagaaa cgatcctcaa tgccctgctg tactcggtgc cgctgctgct ggccgttgtg
    18001 gcccacgaag tggcccacgg cctggcggcc gagaaacggg gggacccgac ggcccggatg
    18061 caggggcgca tcaccatgaa ccccctggtg cacgttgacc tggtgggcac catcctgctt
    18121 cccgggattc tcatcgccac ccggagcccc tttttgttcg ggtgggccaa gcccgtgccg
    18181 gtggacctca ccaacctgaa gggcggccgg agggacatgg ccctggtttc cgcggcgggg
    18241 cccctttcca accttgtgct ggcctgcctg agcgccctga cataccgcgg cgtgatcctg
    18301 gcgatcaatg ctttggggag ccccccggag ctcctttggg tcctggaacc cctgatgatc
    18361 atggcggcat tctccgcgac gttcaacctg accttgatgg tgatcaacct gctgccgatt
    18421 ccgcccctgg acggcggccg catcctcatg ggactgctgc ccccgagact ggccgttcaa
    18481 ctgcagaagg tcgaacccta cggggtcctg attctc
//
LOCUS       DAOOVC010000068        18315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2082_length_18315_cov_5.923439, whole genome shotgun sequence.
ACCESSION   DAOOVC010000068 DAOOVC010000000
VERSION     DAOOVC010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18315
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2082_length_18315_cov_5.923439"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PLM79_10080"
     CDS             complement(<1..1126)
                     /locus_tag="PLM79_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOV86699.1"
                     /translation="MFDQKTLEKIKAGKDKWEEGPVKKTTTKSPERHPVFTTISGTPV
                     ERLYTPNDIAGMDYERDLGFPGQFPYTRGVQPTMYRGRFWTMRQYAGFGSAKDTNERY
                     RYLLQQGQTGLSVAFHLPTQAGYDSDHPLSMGEVGKVGVAIDSIEDMKVLFNGIPLDK
                     VSTSMTINAAATVLLGMYLAIAEEQGVTWDKVSGTVQNDVLKEIICRGQYIYPPKPTM
                     RLTVDLIEFCQKNVPKWNTISISGYHIRESGSTAAQEMAFTIADGIAYAQACVDRGLD
                     VDSFGPRLSFFFNAFTNVLEEVAKFRAARRYWAKVMRDRFGAKDPRSMMLRYHVQTGG
                     VTLTAQQPLNNIVRVALQTYATALGGAQSLHTNSYDEALCL"
     gene            1663..2052
                     /locus_tag="PLM79_10085"
     CDS             1663..2052
                     /locus_tag="PLM79_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOV86700.1"
                     /translation="MIRLQDLVDKIKAEMDPAKVGMIVCHNGVVRATSRKGEPAEYLE
                     IGVDSAAWNQVLEEGRREPGISHVEAVLFTGRREVGEDVLLVAVAGDIRENVFPVLEK
                     TVNRLKKEAVVKKEALLEKERAVGRDH"
     gene            2209..2367
                     /locus_tag="PLM79_10090"
     CDS             2209..2367
                     /locus_tag="PLM79_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86701.1"
                     /translation="MESPCRSCENIHQNKEECSRDCDRIQAFQAAILQLDERNIKDFA
                     LKFNPGGA"
     gene            complement(2629..3612)
                     /locus_tag="PLM79_10095"
     CDS             complement(2629..3612)
                     /locus_tag="PLM79_10095"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOV86702.1"
                     /translation="MAKTPVRVAVTGAAGQIGYAILFRIASGEMLGKDQPIILQMLEL
                     PIDKVQQALQGVMMELADCAFPLLVDMIGTGDPKVAFKDADYALLVGARPRGPGMERK
                     DLLMENAKIFIEQGKAMNEVASRNIKVLVVGNPANTNAWIAMKSAPDLPKGNFTAMLR
                     LDHNRAKSMLAAKTGKPVDSVEKMIVWGNHSPTMYPDIRFCTVDGQPATKLVDENWYK
                     TDYIPTVGKRGAAIIAARGLSSAASAANAAIDHMHDWALGSNGKWVTMGLPSDGSYGI
                     PEDVMYGVPVICTPGKYERVTGLEIDAFSREKMDLTLKELLEEQAGVKDML"
     gene            complement(3655..5658)
                     /locus_tag="PLM79_10100"
     CDS             complement(3655..5658)
                     /locus_tag="PLM79_10100"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOV86703.1"
                     /translation="MDNKEYRHGTSLIAKLIDDPPAQVDNPIKIGDLTFRDGHQSLFA
                     TRGRTEDLLPIAEKMNTVGYWSMEVWGGATFDTMHRFLNEDPWERIRQLKKYLTKTPM
                     MMLLRGQNLVGYRNYADDFAEAFTERAAINGIDVFRTFDALNDFRNFETVIKPIKKLK
                     KHFQGTICYSLTERRMGGPVYNLDYYVKKAKQLEDMGSDTICIKDMAGLIAPYDAYYL
                     VKALKENVKVPIHLHSHFTSGMADLALLKAIEAGVDIVDTCLSPWAYRSSHPAIEPLV
                     VALRGTNRDTGFDLHLLAECSAHFEKVSPKYRHLLDDRMSIIDINVLLHQTPGGMLSN
                     LINQLREMGASDRLNDVFEALPQVRKEMGQVPLVTPTSQIVGIQAVNNVLFDTPQERY
                     KMISAQSKDLFYGLYGETPAPLDPVIQKKALKGYSRGEEPITCRPAEILEPEMPKNYE
                     DTKGLAKDIDDVIIYGMYPMTGMRFLKWKYGKEPIPAEVMPKTLEDCQREAELIKKAL
                     KGELVEKPKKEAPEKGADMRIFSVFVDGEYFQVEVEQTGGPTAAITSIAPMAAPAAAP
                     RPAQVAVAKPAAAAPAAAPAAAAAPAVAVAGGTKLESPMPGMVIRFEVNEGDAIKEGD
                     TIMILEAMKMENSIPSPATGTVKQILAKAGQSVQKGDVLAVIG"
     gene            complement(5904..6104)
                     /locus_tag="PLM79_10105"
     CDS             complement(5904..6104)
                     /locus_tag="PLM79_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86704.1"
                     /translation="MEVKKSVIAAIASAVNLYLTSEQEALMAMQQMARAERPQTLTGL
                     WAIAGRKQAMDIRWTWQMRICR"
     gene            complement(6326..6655)
                     /locus_tag="PLM79_10110"
     CDS             complement(6326..6655)
                     /locus_tag="PLM79_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOV86705.1"
                     /translation="MAKTKAKEDKEKPLDKMTAKELREAALKLEGIVGVHAMNKNELI
                     AAIKEVKGIVDVKSGKKTADVRGLKVKVRELKEKRVRAKEEGNKRLADSLRRKISNLK
                     KKTRKAG"
     gene            complement(6648..7433)
                     /locus_tag="PLM79_10115"
     CDS             complement(6648..7433)
                     /locus_tag="PLM79_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOV86706.1"
                     /translation="MSYGLMVVVVFVVLFLANAIRILNEYERAVVFRLGRVLHSKGPG
                     LIILIPLIDRMQKVSLRLMATDVPSQDVITRDNVSVKVSAVIYFRVIDPVKSIISVEN
                     FLYATSQLAQTTLRSVCGQAELDDLLSERDKINAHIQEILDKHTEPWGIKVTVVEVKQ
                     IDLPIEMQRAMAKQAEAERERRAKVIGAEGEYQAATRLAEAAEIIQQHPVAVQLRYLQ
                     TLREIASENNSTTLFPIPIDLFKPFITMAEMVQQKQNKELENG"
     gene            complement(7487..8794)
                     /locus_tag="PLM79_10120"
     CDS             complement(7487..8794)
                     /locus_tag="PLM79_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HOV86707.1"
                     /translation="MNLKVFPRIILAFLGMILIGSTAARAEHVNIIQIQDSINPGVED
                     FLKYAVSRSEEENAECLIVLLDTPGGLMTSMRGMAQAILNANIPVVVYVSPSGAQAAS
                     AGVFVTAAADIAAMAPGTNIGAAHPVTTGGKDMPSTMSEKVLNDMLAFARSVAAQRGR
                     NADWLEDAVRKSISATAEEAYAKNVIDLIADDLPTLLERLDGWELKHKGTTKTLHTRG
                     IEERTIHPGWQHKVLRAISNPNIAYILLMIGLAGLYFELSQPGVVVPGVIGGISLILA
                     LYAMQTLPVNYAGFLFILLAVVFFLLEIKIASYGLLSFAGVLSLVLGSLMLFRVPGHP
                     TRLAMSVFIPTILTVSGFFVTVAALAFRSQIRKPQTGAEALVGLVGLVEKALAPEGKV
                     FVSGELWNAEADESIPVGEKVVVVSVENLKLKVQKIGGKEVVK"
     gene            complement(8821..9630)
                     /locus_tag="PLM79_10125"
     CDS             complement(8821..9630)
                     /locus_tag="PLM79_10125"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOV86708.1"
                     /translation="MGSEPGRTAAEVRRALRELRYLLTGLQLGGLADWPGSAERIAHN
                     PAVENPTSGGPISLGASPLEAEDPGVAPAPDGEAIPKEDPGELLSEVRTEMGDCRRCR
                     LHSARKQIVFGDGSPRARLVFVGEGPGFEEDQQGRPFVGRAGKLLDKMIRSIGLERED
                     TYICNVVKCRPPGNRTPAQDEMEVCSAFLFRQLKIIAPRVVCALGSCASQTLLGKQKS
                     ISLLRGKRHLWHGVSLVCTYHPAYLLRNPAQKAAVWEDLLEIRSILDANAP"
     gene            complement(9620..10837)
                     /gene="coaBC"
                     /locus_tag="PLM79_10130"
     CDS             complement(9620..10837)
                     /gene="coaBC"
                     /locus_tag="PLM79_10130"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOV86709.1"
                     /translation="MLKGKSVLLGISGGIAAYKAAELVRLLVKEGASVQVVMTANAQR
                     FITPLTLQVLSGQPVCTDLFDLEFESQVGHIQVARAAQLVILAPATANLLGKMAAGIA
                     DDFLTTVLLATTAPVLACPSMNAKMYENPFTQRNLEQLRNMGTHVLGPGLGTLACKEE
                     GIGRLPELSEILESAKRILTPQTLRGKRVLVSAGPTWEHFDAVRFISNPSTGRMGYAL
                     AAVALRRGAQVDLISGPSPLCPPSGVRFHPVKSAEEMRDAVLEVAPGTDAIVMAAAVS
                     DYRPQQVASHKLKKTGKDLEVLLVKNPDILALLGQSRGASSRPVLVGFAAETENLIEN
                     AREKLVKKNLDFIVANDLTQCGSGFACETNQVKILDRSGDITEFPCMAKEEVAVQVFD
                     RIERLLLQETHGE"
     gene            complement(10922..11431)
                     /locus_tag="PLM79_10135"
     CDS             complement(10922..11431)
                     /locus_tag="PLM79_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86710.1"
                     /translation="MAGKKTLERSARATLILFVLTISACSMFGGNTAPPPNPVGQSSQ
                     PPSASSPSSPAPPASSSSAPRPLFFDFPDIPVPQELNRLDSETFVFQSGPLKAGLLTL
                     RGTRVDLGSLVSFFQMAMPRENWKAKGGFHSKRNVLIFEKPDKTAVINIYEKMLRTYV
                     EIYVAPTGS"
     gene            complement(11505..12176)
                     /locus_tag="PLM79_10140"
     CDS             complement(11505..12176)
                     /locus_tag="PLM79_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV86711.1"
                     /translation="MKGRDPLATLAKDLVFERFVVGMLQTNCYLLGDRGSRRAVVVDP
                     GGDCDRIARRITEEKLQLAAILNTHNHFDHVMDAWKLKERLGGEIYLHEKDQPFLRDS
                     ITGMGSFFGLSPGTSDMEIDRNYGDGELLRFGALELRVLETPGHSPGHVSLHFLPAGL
                     VLVGDTLFAGSIGRTDFPGGSYEQLIRSVREKIFPLDPQTMVFPGHGPETTVAHEKRT
                     NPFFR"
     gene            complement(12166..12687)
                     /locus_tag="PLM79_10145"
     CDS             complement(12166..12687)
                     /locus_tag="PLM79_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV86712.1"
                     /translation="MTQELYNDVIKVMTSRRSVRQFLDAPVDRKLIWEALKAASWAPS
                     GLNNQPWRFAVLRDPDLKDRLARLTRYAATFRGAPVLVGVFLDKESSYDYVKDCQAVG
                     ACIQNFLLALASLGLGAVWLGEILKNKEEVLRVLDLPDRLELMAVVAVGHPAHHNQSS
                     HRRPVEELVILER"
     gene            13025..13333
                     /locus_tag="PLM79_10150"
     CDS             13025..13333
                     /locus_tag="PLM79_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV86713.1"
                     /translation="MQWKVIERGHVVAELEGDLNLWSVPEVRDSLLKVARKKSIRRVG
                     VDLRGVRAMDTAGTALLVELHQLLFRRGSDLRLRGVEDHILKMIRLAGLEGAFEMENS
                     "
     gene            13399..14187
                     /locus_tag="PLM79_10155"
     CDS             13399..14187
                     /locus_tag="PLM79_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86714.1"
                     /translation="MNSSDFCTVEGLDSYYGNRQVLYDVRFRIPRNRITVIMGASGCG
                     KTTLLRHLIGLKPCRPGQVLVEGKDLGAFNSRELMLYRRRTGVLFQSGALFNSLSVAE
                     NIAVPLRVHTKLADETIRIICSIKLHMVGLAEFRDYMPSQLSGGMRKRAGLARALAMD
                     PEILFVDEPSSGLDPITAAGLDRLILDLRETLGMTIVVVTHELESAFQIADQLIVLDY
                     GRVLVAGTANEVRGSPDPRVVQFLGRQADSKPPDLGKYRASLLQ"
     gene            14287..15072
                     /locus_tag="PLM79_10160"
     CDS             14287..15072
                     /locus_tag="PLM79_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV86715.1"
                     /translation="MIHWIRKLGAFAIEQVAFLGRCGLFLWLVLRGIGRPPGKTWFVL
                     QQIRFVGSKSLFVIGFTAAFTGMVLGLQGFYTLSKFGSEGLLGSAVALSIIRELGPVL
                     SALMVTGRAGSAMCAEIGIMRIDEQIDALECMAVDPHSYLVTPRLLACLIALPLLTSF
                     FDVVGMVGGYLVGVELLGVNPGSYLDGIHSSVEWEDVFMGFVKSLVFSVLIIWICTYK
                     GYFAGYDEGAYGPEQVSRATTNAVVLASVAILVSDYVVTSILI"
     gene            15085..15540
                     /gene="mlaD"
                     /locus_tag="PLM79_10165"
     CDS             15085..15540
                     /gene="mlaD"
                     /locus_tag="PLM79_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOV86716.1"
                     /translation="MEKRGVELAVGTFLLLGLLCLAYLSIELGKVRWLGYGDYRVEAT
                     FANVGGLKANANVTMAGVQIGKVETIRLKDGRAWVTLSIQGGVKLEEDVIARIKTMGI
                     IGDKYVSITPGASETYIPPGGTIRETQPPLDIEELVGKFVFGSMEKTQK"
     gene            complement(15626..15886)
                     /locus_tag="PLM79_10170"
     CDS             complement(15626..15886)
                     /locus_tag="PLM79_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86717.1"
                     /translation="MKRTLGVLLVVALAFTMISGCACTQDAKKCTELCQSALDKAQAI
                     EASCSASAKAAEAAAARAEAAARRAEAAADKAESIFMKKMKK"
     gene            complement(16154..17116)
                     /locus_tag="PLM79_10175"
     CDS             complement(16154..17116)
                     /locus_tag="PLM79_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOV86718.1"
                     /translation="MRKRLFFLLAPAAVLVLLFPFQILAEEDSEEQFTATVKSYPYRF
                     PDLTVIGSPTWFHVPPKDTLLDIARRFGLGFNEVDLLYPRMDKWLPPNGKKIAVPTFW
                     VLPPTNHQQLVINVPELRIYFFLRNSSTVQTYPIGIGDEGWETPLGTFHITEKRTNPS
                     WYVPLSLQEKYGMAVMPPGPENPLGDYMMKFSAGAYGVHGTAMPWGVGRLVSHGCIRC
                     YPEHIKILFPQVPIGTKLEIIYEPVKLGRVDSQIYVEAHPDVYRKIPDYTDYAVRKLD
                     EHPQAAQVDRKRFLMAIRLQNGVPTNVSRIVSEDISVKMVELGQ"
     gene            17503..>18315
                     /locus_tag="PLM79_10180"
     CDS             17503..>18315
                     /locus_tag="PLM79_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOV86719.1"
                     /translation="MSRGRSDNRPENVKGRFPNSLVPLVNGKLLIQAARKHNAMIMAC
                     NIRCRLPVEGIVLASMAARAPVMYEIAKSELSYTEFTPASFVEFIVSENERLGNTEVP
                     FAVHGDHITVKKEEEVQAVAALIEDEMKAGFTSFAIDASHMENEKNLAATSKLARPIV
                     AAGLNLEVELGEIGAKSGSAEGFTQPEEARWFIGELDKEGIRPDLLAINNGSIHGTYF
                     GAAQEGIQLELTRRIWEAIQPWSVDIAQHGITGTSLDKISSFIQYGIRKGNVG"
BASE COUNT         3657 a         5245 c         5472 g         3941 t
ORIGIN      
        1 gcaggcaaag agcctcgtcg taagagttcg tatggaggga ctgggcgccg cccagggcag
       61 tggcgtaggt ctggagagcc acccggacga tgttgttgag gggctgctgg gccgtgaggg
      121 tgactccgcc cgtctgcacg tggtagcgaa gcatcatgga ccggggatcc ttggcaccga
      181 accggtctct catgactttc gcccagtacc gtcgggcggc acggaacttg gcgacttctt
      241 ccaacacgtt ggtgaaggcg ttgaagaaga agctcaagcg cggtccgaag gaatccacat
      301 ccaggcccct gtccacgcag gcctgggcat aggcaatgcc gtcggcgatg gtgaaggcca
      361 tttcctgggc ggctgtggaa ccggactcac ggatatggta cccgctgatg ctgatggtgt
      421 tccacttggg aacgttcttc tggcagaatt cgatcaaatc caccgtcagt ctcatggtgg
      481 gcttgggagg ataaatgtac tggccccggc agatgatctc cttcaacacg tcgttctgga
      541 ccgtgccgga gaccttgtcc caggtgactc cctgctcttc ggcgatggcc aggtacatgc
      601 ccaaaagcac cgtggcggcc gcattgatgg tcatggaggt ggaaaccttg tccaacggaa
      661 tcccgttgaa gagcaccttc atgtcttcga tggagtcgat ggccacgccc acctttccca
      721 cttcccccat ggagagggga tggtcgctgt cgtatccggc ctgggtcggc aggtggaagg
      781 ctacgctgag gcctgtctga ccctgctgga gcagatagcg gtaccgctcg ttggtgtcct
      841 tggcgcttcc gaagcccgcg tattggcgca tggtccaaaa acggccccgg tacatggtcg
      901 gctgaacccc gcgggtgtac gggaattgcc cggggaaacc caggtcccgt tcatagtcca
      961 ttcccgcaat gtcgttgggg gtgtagagtc tctccaccgg cgtaccggaa atggtcgtga
     1021 agaccgggtg tctctccggc gatttggtgg tggttttctt gacaggtccc tcttcccact
     1081 tgtctttccc ggctttgatc ttctctagtg tcttttggtc aaacatgctt accctcctca
     1141 acgcgatttc cacaggttac cactcgccgt taccctcttg ccggcttccc aaaggccttc
     1201 cccttccttc gtcccccttt gccttggcgg tcccatcccc cccatggccg ggtccttgcg
     1261 gaaggccagc gcaggccgag aagggttcaa tcccataggc acgaaatcta cctcaactca
     1321 gcccagttca gaaaagtaga agatgtaagg ttccacaata tcaaaaaact tttgattgat
     1381 atctatagca gcaagggctt caagaatcaa ctggaatcaa cgggaattag cgctcgtgca
     1441 acggagagga tacccgaaga tcgcaccgta gaaaaagggg tccgacttcc gatctggaaa
     1501 cccataccca tccgtctcgg cgctgtgcta gaatccgcac tgccgaccgc aggtcgtcgt
     1561 cggcaatgcc gaatatgggt gcggggaccg aacccggacc gaccggggtc ctcttcccgt
     1621 gcatgccttt gccccgggcc gagaaaaagg agaaatgcag ccatgatcag gcttcaggac
     1681 ctggtagaca agatcaaggc ggagatggat ccggcaaaag tcgggatgat cgtgtgtcac
     1741 aacggagtgg ttcgagccac cagccggaaa ggagaacccg ccgaatacct ggagatcggc
     1801 gtggattccg ccgcctggaa tcaggtgctg gaagagggcc gaagggagcc gggaatctcc
     1861 catgtggagg cggttctctt caccggtcgt cgggaggtgg gggaagatgt cctgctggtg
     1921 gcggtggccg gggacatacg ggaaaacgtg ttcccggttc tcgaaaagac ggtgaatcgc
     1981 ctgaagaagg aagccgtcgt caagaaggag gccctcctcg agaaggaaag ggcggtgggc
     2041 cgggaccatt gaaagggccc ctctccctcg aagactccct gtccaaaaga atcttgacac
     2101 cccaattccc gtccttcaat atgggaagcc aacagggact gggacttttt cgatgttggg
     2161 ggctgatgga gcggaggtcg agcgatgccg gtgtctggag cgagaacgat ggagagtcct
     2221 tgtcgtagct gtgaaaacat ccatcagaat aaggaagaat gcagcaggga ttgcgaccgg
     2281 atccaggcct ttcaggccgc aattctgcaa ttggatgagc gaaacatcaa ggactttgcg
     2341 ttgaagttca accccggcgg ggcatgatcc aagggcccgg ggaccttccc aggtccctcg
     2401 cggggaaagg gtgtccaccc ctttcaaggg gcgtgggtct ctcttcgctt tctctcgaga
     2461 gcggaggtac cggaaacccg gggtcgtccc cgggcaacgg ggacgtgaga cccgccggga
     2521 aaccgcctcg gcgaaagcgc gaaccgaaaa aaaggaaggg actcgatcca cagtcccttc
     2581 ctttgtcttt ttgaggaggg cggccccccg tcagggaggc ctgccgccct acagcatgtc
     2641 ttttactccg gcctgttctt cgagaagttc cttgagagtg aggtccatct tctcccgcga
     2701 gaacgcatcg atttcgaggc ccgtcacccg ctcatacttg ccgggggtgc aaatgaccgg
     2761 gaccccgtac atgacgtcct cgggaatccc gtaggagccg tccgaaggaa ggcccatggt
     2821 cacccatttg ccgttggagc ccagcgccca atcgtgcatg tgatcgatgg cggcgttggc
     2881 ggccgaggcg gcggaggaca gccctctggc cgcgatgatg gcggccccgc gcttgcccac
     2941 cgtggggatg tagtcggtct tgtaccagtt ctcatccacc agcttggttg caggctgccc
     3001 atccaccgtg cagaaacgaa tgtccggata catggtgggc gaatgattcc cccatacgat
     3061 catcttctcg acggaatcca ccggcttgcc ggtcttggcg gcgagcatgg acttggcgcg
     3121 gttgtggtcc aggcgcagca tggccgtgaa attgcccttg ggcagatccg gggcggattt
     3181 catggcgatc caggcattgg tgttggcggg attgcccacc accaggacct tgatgttccg
     3241 ggaagctact tcgttcatgg ccttgccctg ctcgatgaag atcttggcgt tttccatgag
     3301 caggtcttta cgttccatgc cggggccccg gggcctggcg ccgaccagca gagcgtagtc
     3361 ggcgtccttg aaggcaacct tcgggtcgcc cgttccgatc atgtccacga gaagcggaaa
     3421 tgcgcaatcc gccagttcca tcatgacgcc ttggagcgcc tgctggacct tatcgatggg
     3481 caactccaac atctgcagaa tgatcggttg atctttgccg agcatttcgc cgctggcgat
     3541 gcggaacagg atggcgtaac cgatctgacc ggcggccccc gtcactgcca cacgaactgg
     3601 ggttttggcc atagtaagtc cctccttatg aaagtcgatc cgttggaatt tggattatcc
     3661 gataaccgcc agaacgtccc ctttctgtac actctgaccc gccttggcca ggatctgctt
     3721 gacagttccc gtggcagggc tggggatgga gttttccatt ttcatggctt cgaggatcat
     3781 gatggtgtcg ccttccttga tggcgtcccc ttcgttgact tcaaaccgaa tgaccatgcc
     3841 gggcatgggg gattcgagtt tggtgccgcc cgcaaccgcc actgccggag ccgctgccgc
     3901 tgccggagcc gccgccgggg ctgccgccgc aggtttggcc acggccacct gggcgggtcg
     3961 gggagctgcc gccggggccg ccatgggggc aatggaggta atggctgccg tggggccgcc
     4021 cgtttgctcc acttccacct ggaaatactc gccgtccacg aatacgctga agatcctcat
     4081 gtccgcaccc ttctcgggag cttccttctt gggcttctcg acaagctcgc ccttgagggc
     4141 cttcttgatg agttccgctt cacgctggca atcttccaag gtcttgggca tgacctcggc
     4201 cgggatgggt tccttgccgt atttccactt gaggaaccgc atgccggtca tggggtacat
     4261 gccgtagatg atgacatcgt cgatgtcctt ggccaaaccc ttggtgtctt cgtagttctt
     4321 gggcatctcg ggctcgagga tctcggcggg acggcaggta atgggctcct cgccccgcga
     4381 gtaccccttg agggcctttt tctgaattac cggatccaga ggcgccgggg tctcgccgta
     4441 cagtccgtag aacaggtctt tggactgggc ggagatcatc ttgtagcgtt cctggggagt
     4501 atcgaacaat acgttgttca ccgcctggat ccccacgatc tggctggtgg gggtcaccag
     4561 gggaacctgg cccatttctt tgcggacctg gggcagggcc tcgaacacgt cgttcagccg
     4621 gtcggaggcg cccatttccc gcaactggtt gatgaggttg gagagcatgc cgcccggggt
     4681 ctggtggagc agcacgttga tgtcgatgat ggacatacgg tcgtcgagga gatgacgata
     4741 cttgggagag accttctcga aatgggcact gcactcggcc agcaggtgaa gatcgaagcc
     4801 cgtatcgcgg ttggtgccgc gcaacgccac caccaggggc tcgatggccg ggtgcgaact
     4861 gcggtaagcc cagggggaca ggcaggtgtc cacaatgtcc acccccgcct cgatggcctt
     4921 aaggagggcc aggtccgcca tgcccgaggt gaaatggctg tggagatgaa tgggaacctt
     4981 gacgttttct ttgagggcct tgacgagata gtatgcatcg tagggagcga tgagaccggc
     5041 catgtctttg atgcagatgg tgtccgatcc catgtcttcc agttgcttgg ccttcttgac
     5101 gtaatagtcc aggttgtaca cgggaccgcc catgcggcgt tcggtcaggg agtagcagat
     5161 ggttccctga aaatgcttct tgagcttctt gatgggcttg ataaccgtct cgaaattccg
     5221 gaaatcgttc aaggcgtcga aagttcggaa cacatcgatt ccgttgatgg ccgctctctc
     5281 ggtgaaggcc tcggcgaagt cgtcggcata gttgcggtaa ccgaccaggt tctgtccccg
     5341 aagcagcatc atcatgggag tcttggtgag gtacttcttc agctgtcgaa ttcgttccca
     5401 tgggtcttcg ttgaggaatc gatgcatggt gtcaaaggtc gccccccccc agacttccat
     5461 ggaccaatat cccaccgtat tcatcttctc ggcgatagga agaaggtctt ccgttctgcc
     5521 acgcgtggcg aaaagcgatt gatgtccatc acggaatgtc aggtctccaa tcttgatggg
     5581 attgtccact tgcgcgggag gatcatcaat tagctttgca atcaatgacg tgccgtgacg
     5641 atactctttg ttgtccatcc ttaagtctcc tttttcccta ttttccgttt tccacttgag
     5701 aggttaacct cagccgccca cgtttccagc actcggtttc gcatcctttg gaagatttcg
     5761 caagtttgtc agtcctgcct ctcctcagga tatccattct cggacctggt tgcccattgc
     5821 ctgtttcgca accgctgccg atcatcgcct cgagatcatg gaaagtcccc tggtgctgtc
     5881 gccagaactt cctgtggcgt ctactaccgg catatccgca tttgccaagt ccaccggata
     5941 tccatcgcct gcttgcgccc tgcaatcgcc caaagaccgg tcagggtttg aggccgttca
     6001 gctcgagcca tttgctgcat cgccatcaac gcttcctgct cggatgtgag gtacaggttt
     6061 actgccgaag caattgctgc aatcacgctc ttcttgactt ccatcctttt accctcctta
     6121 ctgcaggtcc cactcttcgt tgcccgttgc ctcgagttct cggtagatcg ccgatggagg
     6181 tcatggccct tgggctcagc tcgatgaaat ccattagcgc tatatgaatc cgctctcggg
     6241 aaacacccag ccccgtcgcg gcgatgcacg gaggaggaat gggtccccgt cggagtccta
     6301 ttgaatcaga ccctgcaccg ggagttcaac cggcctttcg ggtcttcttc ttgaggttgc
     6361 tgatcttcct gcgcaacgaa tccgccagcc gcttgttccc ctcttccttg gcccggaccc
     6421 tcttttcctt caattcccgc accttcactt tgaggccgcg cacatctgcg gtcttcttgc
     6481 cgctcttcac gtccacgatg cccttgactt ccttgatggc ggcaatgagc tcgtttttgt
     6541 tcatggcgtg aaccccgacg atcccctcga gcttgagggc cgcctcccga agttccttgg
     6601 ccgtcatctt gtccaggggc ttttccttat cctccttcgc cttggtctta gccattctcc
     6661 aactccttat tctgcttctg ttggaccatc tccgccatgg tgatgaaggg cttgaagaga
     6721 tcgatgggaa tcgggaaaag ggttgtggaa ttgttttcgg atgcgatctc tctcaaggtc
     6781 tgaagatacc ggagctgaac cgcaacggga tgctgttgga tgatctcggc ggcttccgcc
     6841 aatcgggttg cggcctggta ttctccctcg gcgccgatca ccttggctcg cctctctctt
     6901 tcggcttccg cctgcttggc catggctcgc tgcatttcga tggggagatc gatctgcttg
     6961 acctccacca ccgtcacctt aattccccag ggttccgtgt gcttgtccag aatttcctgg
     7021 atatgcgcgt tgatcttgtc ccgctcggag aggaggtcgt ccagttcggc ctgaccgcag
     7081 acgcttcgaa gggtggtttg cgccagctga ctcgtggcat acaggaaatt ttccacggag
     7141 atgatggatt ttacagggtc gatgacgcga aaataaatga ccgcactcac cttcaccgag
     7201 acgttgtccc gggtgatgac gtcctgagaa ggaacgtccg tggccatgag ccgcaaactc
     7261 accttctgca tgcggtcgat caggggaatc aggatgatga gacccgggcc cttggaatga
     7321 agtactctgc ccagccgaaa caccacggcg cgttcgtatt cattgagtat gcggatggca
     7381 ttggcaagaa acagtaccac gaaaaccaca accaccatga gcccatacga catcgccatc
     7441 ctcctgaagc ccgccatgtt ttgaaatggc taaacaaaag ttgaaactat ttaacaacct
     7501 ctttaccacc gattttttgg actttcaact ttaaattctc aacggaaacc accactacct
     7561 tttcccccac cgggatgctc tcgtcggctt cggcgttcca gagttccccg ctcacgaaaa
     7621 cctttccctc gggggcaagg gccttttcca ccagcccgac gagccccacc agggcttcgg
     7681 ctcccgtctg aggctttcgg atctgggacc ggaaggccag ggccgccacg gtcacgaaga
     7741 atccggacac ggtcagaatg gtgggaatga acaccgacat ggccaggcgg gtcggatggc
     7801 cggggacgcg aaaaagcatg agcgaaccca gcaccaggga caggaccccg gcaaagctca
     7861 aaagcccgta cgatgcgatc ttgatctcga ggaggaaaaa cacaacggcc agcagaatga
     7921 atagaaagcc cgcataattc acgggaaggg tctgcatggc gtacagggca agaatgaggg
     7981 aaatcccgcc gatgaccccg gggacgacca ctccgggctg agagagctcg aagtagaggc
     8041 cggccaatcc gatcatcaac agaatgtagg cgatattagg gttgctgatg gctcgaagaa
     8101 ccttgtgctg ccacccggga tggatggttc gctcctcgat gcccctggta tgcagggtct
     8161 tggtggtgcc cttgtgcttg agctcccagc cgtcgagtct ttccaggagg gtcggaaggt
     8221 cgtctgcaat caggtcgatc acgttcttgg cgtaggcctc ttccgccgtg gcggaaatgc
     8281 tctttcgaac cgcgtcctcg agccagtcgg cgtttcggcc ccgctgggcc gcaacgctgc
     8341 gtgcaaaggc caacatatcg ttgagaacct tctcgctcat ggtggacggc atgtccttgc
     8401 cgcctgtggt cacgggatga gccgctccga tgttggttcc gggggccatg gccgcaatgt
     8461 ccgccgcggc ggtgacaaag acccccgccg acgccgcctg tgccccgctg ggagaaacgt
     8521 acaccaccac cggaatgttg gcattcaaga tggcctgtgc catgcccctc atggaggtca
     8581 tgaggcctcc gggggtatcc aggagaacga tgaggcactc ggcgttttct tcttcggaac
     8641 gggaaacggc gtacttgaga aaatcctcca cacccggatt gatggaatcc tggatctgga
     8701 tgatgttgac gtgttccgca cgggccgcgg tggagcctat gagaatcatc cccaggaagg
     8761 ccagaatgat ccggggaaag actttgagat tcatggcaaa cctctcggtg tccggatatc
     8821 tcaaggggca ttcgcatcga gaatcgagcg gatttccaac aggtcttccc agacggccgc
     8881 cttttgagcc ggatttctca gcaggtaagc cggatggtag gtgcagacca gagaaacccc
     8941 gtgccacagg tggcgtttcc ccctcaagag tgaaatggat ttctgtttgc ccagaagggt
     9001 ttgggaggca caagatccca gggcgcagac gacccgggga gcaatgattt tgagctgtcg
     9061 gaagagaaag gcggagcaga cttccatttc gtcctgggcc ggagtccgat ttcccggagg
     9121 acggcacttg accacattgc agatataggt gtcctcacgt tcgagcccga tgcttcgtat
     9181 catcttgtcc agaagcttgc cggccctgcc cacaaagggc cttccctgct gatcctcctc
     9241 aaacccgggg ccctctccca cgaagaccag gcgagctcgg ggcgagccgt ctccgaaaac
     9301 aatctgcttc cgagccgagt gaagacggca ccgtcggcag tcccccatct cggtccgaac
     9361 ctcgctcagg agttccccgg ggtcttcctt cgggatcgct tccccgtcgg gcgcaggggc
     9421 aacccccggg tcttcggcct ccaaggggga ggccccgagg gatatgggcc cccccgaagt
     9481 cgggttttcc accgccgggt tgtgggcgat cctctccgcg gatccgggcc agtcggccaa
     9541 ccctcccagc tgaaggcccg tcaggagata gcgcagctct ctcagcgccc ggcgcacttc
     9601 ggcagcggtt cttcccggct cactccccat gggtctcctg caacaagagt cgttcgatcc
     9661 ggtcgaacac ctgcacggcc acctcttctt tggccatgca ggggaattcg gtgatgtctc
     9721 cggaacggtc caggattttc acctgattgg tctcacaggc aaaccccgag ccgcattggg
     9781 tcaggtcgtt ggccacgatg aagtccaggt tcttcttgac gagcttctcc cgggcgttct
     9841 cgataaggtt ctccgtctcg gcggcaaagc ctacgagaac gggccgggag gaggcgccgc
     9901 gggactgccc cagcagggcc aggatgtccg ggttcttgac caggagcact tccaggtcct
     9961 tgccggtctt cttgagctta tgggaggcca cctgttgggg acgataatcg ctgacggcgg
    10021 ccgccatgac gatggcatcc gtcccggggg cgacctccaa aacggcatcc ctcatctcct
    10081 cggcgctctt aacggggtga aatcgaaccc ccgagggcgg acaaaggggg ctgggcccgg
    10141 aaatcaaatc gacctgcgcc cctcgtcgca gggcgacggc ggccaatgcg taccccatcc
    10201 tgcccgtgga cggattgctg atgaaccgga cggcatcgaa atgttcccag gtaggacccg
    10261 cactcaccag taccctcttt ccacgcaaag tctggggggt gaggattcgc ttggcggatt
    10321 cgaggatctc cgaaagctcg ggcaaccgcc cgatgccttc ctccttgcag gccagggtgc
    10381 ccaggccggg ccccaggaca tgggtcccca tgttgcgcaa ttgctccagg ttccgctgag
    10441 tgaaggggtt ctcgtacatc ttggcattca tggaggggca ggcaagaacc ggcgccgtgg
    10501 tggccaggag aaccgtggtg aggaaatcgt cggcgattcc cgccgccatc ttccccaaaa
    10561 ggtttgcggt ggccggggcc aggatgacca gctgggccgc cctcgccacc tggatgtgtc
    10621 ccacctggga ctcgaactca aggtcgaaaa ggtccgtgca taccggttgt ccggacaaca
    10681 cctggagggt gagaggggtg atgaaacgtt gggcgttggc cgtcatgacg acctgaacgg
    10741 aggccccttc tttgacgaga aggcgaacca attccgccgc cttgtacgcg gcgattcctc
    10801 cgctgattcc caggagaacg gatttgcctt tgagcatgtt cggcgcctag tgtgaaggga
    10861 gttgaaaggt ttcgcatgag tttccggcgg ttggtgatcc cgttcgcagc tccgcccggc
    10921 tttagctccc cgtaggagcc acataaatct ccacgtaggt gcggagcatc ttttcgtaaa
    10981 tgttgatcac ggccgtctta tcgggtttct cgaaaatgag gacgtttcgt ttggaatgaa
    11041 atcccccctt ggctttccag ttctcccggg gcatggccat ctggaagaag ctgaccaggg
    11101 accccaggtc cacgcgggtc ccccgcaagg tgagcagccc tgccttgaga gggccggact
    11161 ggaacacgaa ggtctcgctg tccaacctgt tcaactcctg gggtaccggt atgtccggaa
    11221 agtcgaaaaa cagggggcga ggcgccgagg atgacgatgc gggtggagcg ggagaagagg
    11281 gggaggaggc ggaagggggc tgggaggatt ggcccacggg attgggaggg ggagccgtgt
    11341 tcccgccaaa catgctgcac gcggaaatgg tcaagacgaa caggatcagc gtggcccttg
    11401 ccgaccgttc cagtgttttt ttgcctgcca tccccattcc tccttacaaa gagtgtcgtc
    11461 cagttccgtg caggattcgc ggatgccggt gtccaggcca cgtcctagcg aaaaaagggg
    11521 tttgttcgct tctcgtgagc cacggtggtc tcgggtccat gaccgggaaa taccatcgtt
    11581 tgcgggtcca gagggaatat cttttcccga acggaacgaa tcagctgctc gtaggatccc
    11641 cccgggaaat ccgtccggcc gatggaaccg gcaaacaacg tatcccctac gaggacgaga
    11701 ccggccggca agaaatggag ggaaacatgg ccgggactat ggccgggggt ttccagtacc
    11761 cgcaattcca gggcaccgaa ccgcaacagc tccccgtccc cataattccg gtcgatctcc
    11821 atgtccgatg tgccggggct cagaccgaaa aacgacccca tacccgtgat gctgtcccga
    11881 aggaacggct gatccttctc gtggagatag atttccccac cgagcctctc cttgagcttc
    11941 catgcgtcca tcacgtggtc gaagtggttg tgggtgttca ggattgccgc cagttggagc
    12001 ttctcttcgg tgatgcggcg tgcgatccgg tcgcagtctc cccccgggtc aacgaccacg
    12061 gctcgcctcg atcctcggtc gcccagaagg tagcaattgg tctggagcat ccccactacg
    12121 aaacgctcaa aaacgagatc cttcgccagg gtcgccaacg ggtccctacc tttcaaggat
    12181 caccagttcc tccaccggtc gccggtggga gctctggttg tggtgagccg ggtgtccgac
    12241 ggcaaccact gccatcagtt cgagacggtc cggcaggtcc agcacgcgca gaacttcctc
    12301 cttgttcttg aggatttctc ccagccacac cgcccccagc ccgagggagg cgagggccag
    12361 caggaagttc tgaatgcacg cgcccacggc ctggcaatcc ttcacgtaat cgtacgagct
    12421 ttccttgtcc aggaacacgc ctacaaggac cggtgctccc cgaaacgtgg ccgcatagcg
    12481 agtcaggcgg gccaggcggt ccttgaggtc cgggtcccgg aggacggcaa accgccacgg
    12541 ttggttgttc aagcccgacg gggcccagga agccgctttc aaggcctccc aaatcagctt
    12601 ccgatccacg ggtgcgtcca gaaattggcg cacgctgcgc cgactcgtca tcactttgat
    12661 cacgtcgttg tacagctctt gtgtcaatcc gtcgtctcct cggacccggg ggtgccacag
    12721 aagattgttt tgtagtattt aacatccgtc ggccgattgg caaaccaagt atagtggttc
    12781 aaaggccgaa tggtcaagac gggcctgaaa actaccggta tcccttgatc cgaacttata
    12841 tagcagaatt ctcagccttt cacgaaaatt catcttgtga agtctgattt taccattact
    12901 atctatgcgc ttgtccgttt gtcgcggttg gaggggggtc ctcttcgatg ctgctgaagg
    12961 gcaaccgcag gccaccgggc gacggcgaaa cccggaggtt ttcccatggg gggaggagat
    13021 gcgggtgcaa tggaaggtga ttgaaagagg ccatgtggtg gcggaactgg aaggagacct
    13081 gaacctttgg agcgttcccg aggttcgcga tagccttttg aaggttgccc gcaagaaatc
    13141 gattcgcagg gttggagtgg acctgagagg ggtgcgggcc atggacactg ccgggacggc
    13201 cttgctggtg gagcttcatc aactcctgtt tcgtcgaggc tccgaccttc gcttgagggg
    13261 ggtcgaggat cacatcctca agatgattcg acttgccggt cttgaaggcg ccttcgagat
    13321 ggagaattcc tgaccctccc ggcatgagct ccaccaagga aggccggatc tgcattctca
    13381 tattgagcag gcgggaacat gaattcctcg gatttttgta cggtcgaagg tctcgacagc
    13441 tactatggaa accgtcaagt cctttacgac gttcgtttcc ggattccccg caatcggatt
    13501 accgttatca tgggggcaag cggttgcgga aagaccaccc tgttgcgtca cctcatcggg
    13561 ctcaaaccgt gccgtcccgg gcaggtcctg gtcgaaggca aggacctggg ggccttcaat
    13621 tcccgggagc tcatgctcta ccggaggcgg accggggtgt tgtttcaaag cggggcgctc
    13681 ttcaattcct tgagcgtggc ggagaatatc gccgttccct tgagagtgca cacgaagctc
    13741 gccgatgaaa ccatccggat catctgttcc atcaaactgc acatggtggg attggcggaa
    13801 ttccgggact acatgccttc gcaattgagc ggaggcatgc ggaagcgggc cggacttgcc
    13861 agggccctgg ccatggaccc ggagatcctc tttgtggacg aaccctcctc gggtctggac
    13921 cccatcaccg ccgccggcct cgaccgcctg atcctggacc tgcgggaaac cctgggcatg
    13981 accattgtcg ttgtgaccca cgagttggag agcgcctttc agatcgcgga tcagttgatt
    14041 gtcttggatt atgggagagt ccttgtggcg ggtaccgcga atgaggttcg ggggagtccg
    14101 gacccgcggg tggttcagtt cctgggcaga caggccgatt cgaagccgcc ggacttgggg
    14161 aagtaccgtg cctctttgct gcaatgacgg agggcgcgtt ccgtttgggc aacttcggac
    14221 aagggacaaa tcgatccggg cgcgcccggc gggcgaaggg gtagggggcc ggaggaagga
    14281 acgggtatga ttcactggat acggaagctg ggagcctttg ccatcgaaca ggttgccttc
    14341 ctgggacgtt gcgggttgtt tttgtggctt gtccttcggg ggatcggccg tccacccggg
    14401 aagacgtggt ttgttttaca gcagatccgg ttcgtgggga gcaagtccct ttttgtcatc
    14461 ggctttaccg cggccttcac cggcatggtc ctgggcctcc aggggttcta caccctatcg
    14521 aaattcggtt ccgaggggct cctgggttcc gcagtggccc tgagcatcat ccgggaactg
    14581 gggcctgtgt tgtccgccct gatggtgacg ggaagggcgg gctcggccat gtgtgcggaa
    14641 atcggcatca tgcgcatcga cgagcagatc gacgccctgg aatgcatggc cgtggatcct
    14701 cactcgtacc tggtcacgcc ccgccttctg gcatgcctca tcgccctgcc gctcctcacc
    14761 tcctttttcg acgtggtggg aatggtgggg ggatatctgg tcggcgtgga attgctgggg
    14821 gtgaatcccg gttcctatct ggacgggatt cactcgagcg tggaatggga agacgttttc
    14881 atgggcttcg tcaagtcgct ggttttctcg gtcctcatta tatggatatg tacctataaa
    14941 gggtactttg cgggctatga cgagggggcc tacgggccgg agcaagtaag ccgggccacg
    15001 accaacgcgg tcgtccttgc gtcggtggcc atcctggtga gcgattacgt ggttacttcc
    15061 attttgatct agcggagttg catcatggaa aaaaggggag tcgaactcgc ggttgggacc
    15121 ttcctgctgt tggggcttct atgtctggcc tatttgtcca tcgagctggg gaaggtccga
    15181 tggctcggct acggggacta tcgggtggag gcgaccttcg ccaatgtggg tggattgaag
    15241 gccaatgcca atgtgaccat ggccggagtt caaatcggaa aagtcgagac gattcgcctc
    15301 aaagacggcc gcgcgtgggt aaccctgagc attcaagggg gtgtgaaatt ggaggaagac
    15361 gtcattgccc ggatcaagac catgggaatc atcggagaca aatacgtgtc catcaccccc
    15421 ggcgcatccg aaacctacat tccgccgggg ggcaccatcc gagagaccca gccgcccctg
    15481 gatatcgagg aactggtggg aaagtttgtg ttcggcagta tggaaaagac ccagaagtga
    15541 tcgaagcgga aggtgccgaa agcaggaaag ccggcgagaa ccccgccggc tttcttgttg
    15601 gaacaaagga ttgcaggtac cgaaactact tcttcatctt cttcatgaaa atggattcgg
    15661 ctttgtcggc tgccgcttcg gctcgacggg ctgccgcttc ggcccttgct gccgccgcct
    15721 ctgcagcctt tgcgctggcg ctgcaggatg cttcgatggc ctgagcctta tccagagcgg
    15781 actggcagag ctcggtgcac ttcttcgcat cttgcgtgca tgcgcaaccg gagatcatgg
    15841 tgaatgccaa agccacaact aacaaaactc ccaatgttcg tttcatttct ctttcctcct
    15901 taaaagctga ttgatgctat gatgaaacct accctctcca ctaaagctac tcagggcgct
    15961 ctcctctcac ctcccctcct acagcatgca ttttaagaga atagttcctc gcagcttgtt
    16021 cagactccct catactagcc cggaaaaacg gaggaaatgc tactctacat gttgtccaaa
    16081 atcaagaaga aattcaaaga aaatccgtcc actcaacgat gtgcccctgc cttagaggta
    16141 cctattagca ctgttactga ccaagctcaa ccatttttac ggaaatatcc tcggagacaa
    16201 tgcggctgac gttggtgggg accccgttct gaaggcgaat ggccatcagg aaacgcttgc
    16261 gatccacttg ggccgcctgg ggatgttcgt ccagttttct cacggcgtag tccgtgtagt
    16321 ccggaatctt gcggtagacg tccggatgag cctccacgta gatctgggaa tccacccggc
    16381 cgagcttcac cggttcatag atgatctcca gcttggtgcc gatgggaacc tgcgggaaga
    16441 ggatcttgat gtgctccgga tagcagcgaa tgcacccgtg ggaaaccaat ctcccaactc
    16501 cccaaggcat ggcggttccg tgaactccgt aagccccggc gctgaacttc atcatgtagt
    16561 ccccgagagg attttcgggc cccgggggca tgacggccat cccgtacttt tcctggaggg
    16621 aaagaggcac ataccaggaa gggttggtac gcttttccgt gatgtgaaac gtgcccaggg
    16681 gtgtctccca accctcgtcc ccgatcccga tgggataggt ttgtaccgtg gaggagttgc
    16741 gaaggaaaaa gtagatcctc agctcgggaa cgttgatgac gagctgttga tggttcgtgg
    16801 ggggcagcac ccagaaggtc gggaccgcaa tcttctttcc gttgggcgga agccatttgt
    16861 ccatgcgcgg gtacagcaga tcgacctcgt tgaaacccaa tccgaaacgc cgggcaatgt
    16921 ccaaaagggt gtctttgggg gggacatgga accaggtggg agatccgatc accgtcaggt
    16981 ccggaaaccg gtaggggtac gattttacag tggccgtgaa ctgttcctcc gagtcttctt
    17041 cggccaagat ctgaaaggga aacaggagca ccaggacggc tgccggcgcg agcaggaaaa
    17101 ataatcgttt cctcatgaat ccttcatcct aagggttgga ccccatggat cggccccttg
    17161 aaaagccgca cgtacaatag agattccctc gggtcagatc aagcgttttt tgccatttcc
    17221 gtccaaatct gccggagggt ttccataagt cccatgacgg accggtccgg gcttcccgtc
    17281 tatcccgcaa ccccagcggc ttgaacttta gcgaagaatc ccctataaga caagaggtga
    17341 agacacccgg tccccctaca gccgtgcccg aacgatctgc gccgaggggg accgtgccgt
    17401 acaaggcccc tttgggattc aaagggaatg cacggagcag gcgtgggagc tccgtaggaa
    17461 taccctcgga tggaaagatc agaccgcatg gggagggtca acatgtcaag aggcagaagc
    17521 gacaatcgtc ctgaaaatgt caagggcagg tttcccaaca gcctcgttcc cctggtgaac
    17581 ggaaagctgc tcattcaagc cgccagaaag cacaatgcca tgatcatggc ctgcaacatt
    17641 cgctgccggc ttccggtgga gggcattgtc ctggcttcca tggccgcccg ggcacctgtc
    17701 atgtacgaga tcgccaagtc ggagctttcg tacacggaat tcaccccggc ttctttcgtg
    17761 gagttcatcg tttcggaaaa cgaaaggctg ggaaataccg aagtcccctt tgccgtgcac
    17821 ggggatcata tcaccgtgaa gaaggaagaa gaggtccagg ccgtggccgc cctcatcgaa
    17881 gacgagatga aagcggggtt cacttccttt gccatcgatg catctcacat ggaaaacgaa
    17941 aagaacctgg ccgccacgtc caaactggcc cgtcccatcg ttgccgcagg tctgaacctg
    18001 gaagtggaac tgggagagat tggggccaag agcggcagtg cggaaggatt cacgcaaccc
    18061 gaggaagccc ggtggttcat cggggaactg gacaaggagg gcattcgccc ggacctgctg
    18121 gccatcaaca acggctcgat ccacggaacc tattttggag ccgcccagga ggggatccaa
    18181 ctcgaactga cccggcggat ctgggaggcc atccaaccct ggtcggtaga cattgcccag
    18241 cacggcatca cgggaacttc cctggacaag atttcctcct tcatccaata cggaattcgc
    18301 aaggggaatg tggga
//
LOCUS       DAOOVC010000069        18239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2091_length_18239_cov_6.839034, whole genome shotgun sequence.
ACCESSION   DAOOVC010000069 DAOOVC010000000
VERSION     DAOOVC010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18239
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_18239_cov_6.839034"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            513..2630
                     /gene="aspS"
                     /locus_tag="PLM79_10185"
     CDS             513..2630
                     /gene="aspS"
                     /locus_tag="PLM79_10185"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOV86720.1"
                     /translation="MNGNHRSYCGNLTLDDVDQEVCLAGWVDALRDHGEVLFIHLRDR
                     TGILQVVFSPEFASSRTCEEAGHLRSEFCIRVKGRVHRRAPGTENPHIETGNLEVVAR
                     DLEVLSRAASLPFAISEKAMTAGGTTGRMDAVSEDLRLQYRYLDLRRPSMQELLVQRY
                     RILQCARNVLDERGFVEIETPMLTKSTPEGARDYLVPSRLHPQHFYALPQSPQLFKQL
                     LMVAGFERYFQVVRCFRDEDLRPNRQPEFTQLDIEASFIDEEFIYDLTEELAARMFQA
                     AGIRLPRPFPRMTYREAMDLYGTDRPDTRFDLTFVEATDLFRETRYGIFKQILDRGGR
                     IKGICVRGQSENLSKNVLQNEYAKEIVPSFGARGMTWMRVADGDLDSNIVQFFSPREK
                     QAVLDRFAAKDGDVVLMIADASYDTVVSALGQLRLHLAARLDLIPRGIYRPLWITEFP
                     LFTQTEEGIASNHHPFTSPDREDFDPADSDQLLQLLSRAYDLVVNGEELGGGSIRIHH
                     KDLQMRVFEALGLSAEEVEEKFGFFLRALDYGAPPHGGLALGMDRMVSMILGTSSIRE
                     VTAFPKNRSAFCPLTRAPSTVSREQLKELGLLDRGETQPMPGPRDRKDDLDHLAWVSR
                     IGFSEEERPLLAAALEDAKKMARTAGLRAPREGPLFSVCPVTDPLKEQAEPVLCPLSA
                     SGELLRCAPSVKGNYYKVASILE"
     gene            2683..3900
                     /locus_tag="PLM79_10190"
     CDS             2683..3900
                     /locus_tag="PLM79_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOV86721.1"
                     /translation="MHSDVSNPGNSAHGTAARNASPAGKRVGVPSNLSVKGLPTEAGS
                     LALRGFTAFEDATVVRRLKECGAQPVSWALSRELGLGLCPEDLSRVLGNGQVDFSLMT
                     DTLGEARVAAAVNGVFGFKPSYGMVSRFGLVGVVPSMDCCGFLGKNLDDISAAFRAVR
                     GKDDRDPSMPDPEACAAADPPESLDSPVSVGTIVQCMNGLDSAERTALEKALSRLESL
                     GATVREVEFQGFDLARLVHNVVGSVEASSSCGKFDGVRYGHRASGAKNWNEMYLRSRA
                     ESFGTLVKAYLFQGAYFQFQDYPAFENACRIRSGLVRESRRLWEQADLVVLPTRRPGA
                     APSNPADVGELYDPFSLTLWSNVTGLPSLSLPRLVLHGDSDLGLQLVGPLFGDERLLR
                     VAGQWFQTMEGVR"
     gene            3897..5330
                     /gene="gatB"
                     /locus_tag="PLM79_10195"
     CDS             3897..5330
                     /gene="gatB"
                     /locus_tag="PLM79_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOV86722.1"
                     /translation="MIMDYEAVIGLEIHVQLNCRTKMFCRCSTRVGDEPNRNTCPICL
                     WLPGYLPRFSRESLERAVLACLSLHCEIRKESAFDQKVYYYPDLPKGYQLSQFHQPLA
                     LNGWLDIPCGDGAAKRIRIRKIHMEEDVAKLVHENEGRTPVSLVDFNRAGVPLVEIVT
                     EPDIRSPREAMEFLRALRIQLRYSESAECSMEQGTMRVDANVSLRPRGSSALNTKVEI
                     KNMNSIRSVGDAVAYEIQRQETCLAAGEPIVLHTRLWDPDNGATLPMREKFEGPCVPD
                     PSVPRIVIEDAWLEDLKARLPEMPAGKTDRFVKQYQLSREEASFLASERELSDYFEAT
                     AALGASPRTCVQWISGQLLPLLKEGNIALTDCPAAPDRLAGLLGLLDREEINANAAKE
                     VLRRMMTDPSPPERIVEECGFRQVSAEGELEALVDRVLAENPSAVENYRKGATKAMGF
                     LMGRAMQASGGKANPRLLKEILARKSA"
     gene            complement(5436..5804)
                     /locus_tag="PLM79_10200"
     CDS             complement(5436..5804)
                     /locus_tag="PLM79_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86723.1"
                     /translation="MEGELRIGTVMDQLAMEVFQAHWSRLVRESSGYLIDAVWGNEAS
                     GALTPIQREIHQRVILALDRVMAGFWAQSLGDVPREAIKNLVQEMLVWRLLYAFELFR
                     RRLSEEEWESDLPNRNKVYN"
     gene            complement(5917..6666)
                     /locus_tag="PLM79_10205"
     CDS             complement(5917..6666)
                     /locus_tag="PLM79_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOV86724.1"
                     /translation="MMNAQEKGEEVREERRNPGSNRGRELLGIIPTLTHQLNNSLAAI
                     VGCAELCLLQPDNTGAVQGRLEKILGATRRIQGINQLIVDLTREIMCPLGTVKVGALI
                     EMGIAACRGLISREGIAVQIMVDPGELNVPGTKSALQRVFTNLILNAVEAMEGRPEKL
                     LKIRAEKEESTSMIRVTIEDTGCGISEERLEEVFQPHYTSKPDGMGLGLCVAREILGG
                     HGGSLSLKSVLGRGTVVELRLPAHRPDCHRG"
     gene            complement(7268..9061)
                     /locus_tag="PLM79_10210"
     CDS             complement(7268..9061)
                     /locus_tag="PLM79_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOV86725.1"
                     /translation="MKAISLGLRGRIAELKQFGWSGRFRGTFLSIVVGVIAGLGAILF
                     EELLRQALHYFLFLSTGFKEPLQGSEAAAVSALASVRSWLFLVIPTLGGLAAGLLVFF
                     TAPEAEGHGTDAMIDAFHRRGGHIRKRVPLVKILASAITIGSGGSAGKEGPIAQIGAG
                     FGSVLATILRLRPRDRRILVLAGASGGIGAIFHAPLGAALFAPEVLYRGTDFESDAIL
                     PCIISSIVASSIFDQYFGRQALFFPGPVDFSVIELPAYALFGMVCAGVGYVYIKVFYG
                     SRDRVFRPVRIPKMLKPALGGFLLGLTALLCPPIMDGGYGWIQAAMEGKIFWGTMLLL
                     VFMKIAATSCTISSGGSGGVFGPSVFMGAMLGGAFGFLGHGLAPQYVVHPSSFVVVGI
                     GGFFAGVAKVPVSSIIMACEMCGNYALLVPLMLVSALSTLALGKTGLYEKQTGSRLTS
                     PAHMGEFAEQILEQLEVRQAVTLRPVTQIRESTPFHELLKIVTASQELHFPVVNGDGK
                     MTGILSLNDIRQVMLEEGIGDLIVAKDVATPNVVCVFLDESLKSALQKMIQIDVDELP
                     VVCRQAPDSIITMISKRDIISHYYERSRSLL"
     gene            9688..11091
                     /locus_tag="PLM79_10215"
     CDS             9688..11091
                     /locus_tag="PLM79_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOV86726.1"
                     /translation="MGGLFGVVSKGDCVSDLFYGTDYHSHLGTRRGGMAVRNAEGIQR
                     SIHNIENTYFRTKFEPELATFRGNCGIGVISDTDPQPLIVGSHLGTFGIATVGKIANL
                     EELIRTALGRRRSFSDTSSGTINPTEMVAKLICEEDCFENGIRKVQALVRGSCSMLLL
                     AGEGIYAVRDRLGRTPLVLGRRDGAVAASSESCAFPNLGFETERFLGPGEGVFLSADG
                     WETRIEPGPAMQICAFLWVYYGYPASEYEGINVEFARNMCGAALARNDSVPVDYVAGI
                     PDSGIGHALGYATERQLPYRRPFVKYTPTWPRSFMPQSQQLRDLVAKMKLIPVRKLIQ
                     DKRLLFCEDSIVRGTQLKDNIRVLYSYGAREVHMRPACPTLIYPCEFLNFSTSRSTLD
                     LVGRQVIREIEGTEDTDLEAYAAHGSEKNLAMTEEIRRRLHLTSLKYQRLEDLVASIG
                     LPKERLCTHCWDGSSYF"
     gene            complement(11475..11777)
                     /locus_tag="PLM79_10220"
     CDS             complement(11475..11777)
                     /locus_tag="PLM79_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86727.1"
                     /translation="MEKSSLHRTNYQTLLELARAYNIKVVEAHGLDTRKGVRFRRDGL
                     DWIALDTDLAACEKTRTLGYLLRNEPKSVASKAKIEGDFSSGSGLSPVLTLCCPTR"
     gene            complement(11847..13586)
                     /locus_tag="PLM79_10225"
     CDS             complement(11847..13586)
                     /locus_tag="PLM79_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOV86728.1"
                     /translation="MRKPLPYDLRLTTTGSGVGYFAAVPALEASFDQDLAYLRECPMD
                     QFMHNHLLEMVGRLDLETARQILEMAGDRDPVVTVLLYEASLTFPALAPLGAGFQPRE
                     IARLLEYTPLNHARWSLLEDRTLHSRWIELFDRNLSQHEPLPSPDDAGLPFPYDPTEP
                     ARGASPPVHVGEICGGVARPSGGKAPADPPPGETARRALERLQALGVVAGEETRHTSS
                     LSPHGFLRRWKMERTVENGCLHYSLSGIQTSYGKGPTPDAARASYAMEMAERCSSFAS
                     IGPEGVLGTRRKYPLVHDRLSRLREQGRAALDPNELPLEAPYADQALYWMEGEERKGG
                     TTRPVLVPVQCVFLFCNLDEISLFSGLGSTGLASGNSREQARVSALLEDLERHAEATI
                     PYHPRHCFRLESRDPEIADLLADYEKRGIHVRFQDLTTELGIPCYKSFVVGPRGEIVK
                     GTGAHLDGRKALLSALMETPHPYPGGPHATRKSEDLPTRVLEELPDWDRGSWKENLDV
                     LERLLEANGFRAVYVDLTREDLGIPVVKAIVPGMELTADFDRFSRVSRRLFVNYWKDF
                     RRENPARGTDPTR"
     gene            13773..15296
                     /locus_tag="PLM79_10230"
     CDS             13773..15296
                     /locus_tag="PLM79_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HOV86729.1"
                     /translation="MVTRVKSCLLLLAFFPAFFPFSAGGTPSEDEDVTARRGKPLGGQ
                     GTEMPVLTLSKPEGGWTSAMQIEVAGTCSDPTADPVTVTLNGVRYFVRSTQGSFARKF
                     PAAKGRNTLIAECSNRAGTARATAEVEAVIPPIPLKVVLTNDTDSAYTDLHVYEPDKS
                     HVYWAKTHSPSGAVFFLNQQDGSFDQAGYGPYLYVHPAPPPGVFRVDANYWPGGAVRH
                     TLANLDIVTDEGLPTESRRRVRTPLARPGETQTLAYIVIRGNNQPPLVYVPGQDPPSD
                     MPPEVKEYQEKGEPRESSDEESAFLHPPDEKSLRESVSRLALLQARKISPNWEKKQRD
                     CSGLVRFAFREALEPRTRRQGEEHGIPGALHLPALSPASRKLFPHYPEIWQIGYTREG
                     TPFFGSFADAETLLGFNFRRKGRDLGLARNADLLVFRKAYESDQPYHLMIFVENRPDA
                     LAVYHNGASGAEAQVRVVRVGDLMESPDPVWLPTMNNPHFLGVYEWNRLRPKDPTAS"
     gene            15257..16981
                     /locus_tag="PLM79_10235"
     CDS             15257..16981
                     /locus_tag="PLM79_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86730.1"
                     /translation="MEPTKTQRPNRILTAAVLGTLAAGLVFYWATSRLQATRLHQMVR
                     SDLPVLDLELEFIDAQGEKVPPDRTRLGGKPPEPRAKTPEPSPEECPELVGGDRTWGL
                     QPSLDEKRPDHCPKTARWKVRRHPVAFSLYFLDGPRLLSFLENDPRAKELVGSKLVQG
                     LFYDPIHAGRVRARDLQLEGIEGAVLSTLVREALAARAELHYDMSHGRKGFVFSFVRN
                     ECPFASRLLPLIGARLARSGYRIPSLKEPVLEMRIGPQRLFLTQVEDRVYLANSLEGL
                     LNVLESLPPRPVDLPKTPLVLTMRGEAFLEKVLPVLFGSETWQAHLGIGLSAEDPGVV
                     EFPLGKAARHLSPRIFKGVLAGIPRDVFASAAAGFHIPPDMTPEQWRRLAVEGPGEPS
                     GNLPGEAGVALVWDLSSQDGPITQVGLAVAAQTAPDKAEGFKRYLADPELTAVCGGGT
                     VFLAASSRNLLVRMKESCEAQSLSVLDWDRGPVRDELEKAQVFVSLNPGAGLKELLLA
                     GGAKAGDTGDFAPEWKQQYEKAKEAMRFEGEWVSRALPVLAYAGSGGLPNEPARLKQV
                     RVREGGSR"
     gene            16978..>18239
                     /locus_tag="PLM79_10240"
     CDS             16978..>18239
                     /locus_tag="PLM79_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOV86731.1"
                     /translation="MNARPWVTAMSAALLVSLCLALPVAQSGSPFYLTVERSFSNTEK
                     PRLRLDYTDPARPILLRVLRPQNVERFLDGQLHISRSYEQPLSELNPGHYVVRGINRA
                     ESPLRGLRDMLDPGFRKTLQGTSFNRAIVETTSGELASPPQQVIHNAPAGFSTVREDY
                     LDLQFGGKGTRDLGWWFGDSALREDQYKIRWIELDPLSDGLYLVQAVQGKAEAQCLLQ
                     VSSLSVQVKQSSEQLAVRIVTRTQEPVAEAAVSYRDGRGKWIFLDRKTDARGEVVFTS
                     PGGALDGKLLVRAETADGRRAFADTDFLPAVSRDDSVFIVTDRPIFKPGETFSYKGVV
                     RSFDEGELKIPEFRQKDARISLIRADGKATDLASTVPLSGFGSFSGSFDLDEAETPGL
                     YRLVAEIDGKPYGGEFRVRDYVKPTFYL"
BASE COUNT         3499 a         5841 c         5574 g         3325 t
ORIGIN      
        1 ctgcccttta cccacatctg cgatcaggcg gggatagatc gccgcctccg agcgaacccg
       61 tagagttcgc aaccattcgc cgtgggtcga ggccccaagg ccgatccccc ggaacaggtc
      121 tcaagaggaa atcgacccat aggcccgcag aaccgtccca tggacgagag tgggattgcg
      181 ccccacaacg cctggttggg gcgaccgccc ggtcgaccct accccctttg cccgcacgaa
      241 acacgccata cggagacaag acccctgagg tcagccatac ggagacctga cccccgtcat
      301 gcccgagacc aactgtcggt tcagtttggc ttaacgtgca tctcatgcta ttagtggcgc
      361 tgatatgcgc gggccttttg agagggacat gttgccccat ggatttgcat tcgggggacc
      421 gcccaacgcc cggagaatcc aggaacccca ttctccggtt catggagact tcccggcggc
      481 cgatcccgat accggaaaag agcggacaca ccatgaacgg aaaccatcga tcgtactgtg
      541 gaaacctgac cctcgacgac gtggaccaag aggtttgcct ggccgggtgg gtcgatgccc
      601 tgagggacca cggagaagtg cttttcatcc acctgcgcga ccgcacgggg atcttgcagg
      661 tggtcttcag ccccgaattc gcttccagcc gcacgtgtga agaggcgggt catttgagga
      721 gcgagttctg tatccgggtg aaaggccgcg tccaccgtcg ggccccgggg acggaaaacc
      781 cccatatcga gaccggcaac ctcgaagtgg tcgctcgaga cctcgaggtc ctgagccggg
      841 ccgcctcgct tcccttcgcc atttccgaga aggccatgac ggcgggcggc accaccggac
      901 gcatggatgc ggtttccgaa gacctgaggc tccagtatcg ctacctggat cttcgcaggc
      961 ccagcatgca ggagctcctc gtccagcgct accgcattct ccaatgcgcc aggaacgtgc
     1021 tggacgaacg gggatttgtc gaaatcgaaa cccccatgct caccaagagc accccggaag
     1081 gggcccggga ttacctggta cccagccgcc tccacccgca gcatttctac gctctccccc
     1141 agtctcctca gctcttcaaa cagctcctca tggtggccgg attcgagcgt tacttccagg
     1201 tggttcgctg ctttcgagac gaggacctgc gtcccaaccg acagccggaa ttcacccagc
     1261 tggacatcga ggcgtccttc atcgacgagg agttcatcta cgatcttacc gaagagctgg
     1321 cggcgcgcat gttccaggcg gcgggcatcc gcctgccccg cccctttccc cgcatgacgt
     1381 accgcgaggc catggacctc tacggtacgg accgccccga cacccgcttc gacctcacct
     1441 tcgtggaagc caccgacctt ttccgggaaa cccgttacgg catcttcaaa cagatcctgg
     1501 atcgcggcgg acggatcaag gggatctgtg tccgggggca atcggaaaac ctgagcaaga
     1561 acgtcctcca gaacgagtat gccaaggaga tcgtgccctc cttcggcgcc aggggcatga
     1621 cctggatgag ggttgccgac ggcgatctcg actcgaacat cgtccagttc ttcagccctc
     1681 gagaaaagca ggcggtgctg gatcgattcg ccgccaaaga cggcgatgtc gttctcatga
     1741 ttgccgacgc atcctatgac accgtggtgt cggccctggg gcagcttagg ctccacctgg
     1801 cggcccggtt ggatctcatc ccccggggaa tctatcgtcc cttgtggatc accgagttcc
     1861 ccctattcac ccaaaccgag gagggcatcg cctccaacca ccatccgttt acgtcccccg
     1921 accgggaaga tttcgacccc gccgactcgg atcagctcct gcagctcctc tccagggcct
     1981 acgatttggt ggtgaacgga gaggaactgg gcggaggcag catccgcatt caccacaagg
     2041 acctgcagat gcgggtcttc gaggccctgg ggctgtcggc ggaagaggtg gaggaaaaat
     2101 tcgggttctt cctccgggcc ttggactacg gagccccgcc ccacggggga cttgccctgg
     2161 gaatggaccg catggtttcc atgattttgg ggacttcgtc catccgggag gttaccgctt
     2221 ttcccaagaa ccgcagcgcc ttctgtcccc tgacccgggc tccttcgacg gtttcccggg
     2281 agcaactcaa ggagttgggg ctcctggacc ggggggagac ccagcccatg ccggggcccc
     2341 gggaccggaa ggacgacctg gaccacctgg cctgggtttc ccggatcggt ttcagcgaag
     2401 aggaacggcc cctcctggcg gcggccctcg aggacgccaa gaaaatggcc cggaccgccg
     2461 gacttcgagc tccccgggag gggcctctct tctccgtatg tcccgtgacg gaccccctca
     2521 aggagcaggc cgagcccgtt ttgtgccctc tttccgcatc cggcgagctt ctccggtgcg
     2581 ctccttccgt caagggaaat tactacaaag tggcgagcat cctggaatga catccgtccc
     2641 gggagcctgt ggagtccacc attcaacaag ggagtgagtt gcatgcattc agacgtctcc
     2701 aacccgggga actcagccca cggaaccgcg gcaagaaacg cctccccggc cgggaagcgg
     2761 gtgggagtcc cgtccaacct ctcggtgaaa ggcctcccca ccgaagccgg gtctttggct
     2821 ctccgcggct tcacggcctt cgaggacgcc accgtcgtcc ggcgtctcaa ggagtgcggt
     2881 gcgcaaccgg tgagttgggc actctcccgg gagctgggct tgggcctctg cccggaggat
     2941 ctctcgaggg tcctgggcaa cggccaggtc gacttctccc tcatgacgga caccctcggg
     3001 gaagcgcggg tcgcggcggc agtgaacggg gtgttcgggt tcaagcccag ttacgggatg
     3061 gtatcccggt tcggcctggt gggcgtggtt ccttccatgg actgctgcgg tttcctggga
     3121 aaaaacctgg atgacatttc cgcagccttc cgggcggtcc gaggaaaaga cgaccgagac
     3181 ccctccatgc cggacccgga agcctgcgcc gcggccgatc ccccggaatc tttggattcc
     3241 cccgtatcgg tgggaacgat cgtccagtgc atgaacgggt tggattccgc cgagcggacg
     3301 gctctcgaga aggcgctgtc ccggttggag tccttgggcg cgacggttcg ggaggtggaa
     3361 ttccaaggct tcgacctggc ccggttggtc cacaacgtgg tcggttccgt ggaggcgtcc
     3421 tcgagctgcg ggaaattcga cggggtacgc tacggccatc gggcctccgg ggcaaaaaac
     3481 tggaacgaaa tgtacctgcg ttcccgagcc gaatccttcg gcacgctggt gaaagcctat
     3541 ctcttccagg gtgcctactt ccagttccag gactacccgg ccttcgaaaa tgcatgccgc
     3601 attcggtccg gcctggtccg ggaaagcagg aggctgtggg aacaggcgga cctggtggtt
     3661 ctgcccaccc ggcgcccggg cgccgcccct tccaaccccg ccgacgtcgg agagctctac
     3721 gaccccttca gcctgaccct gtggtccaac gtgacgggcc ttccctccct gagccttccc
     3781 cgtctcgtcc tccatgggga ctcggacttg ggcctccagc tggtgggccc cctgttcggc
     3841 gacgaacggc tcttgagagt ggccgggcaa tggttccaaa cgatggaagg agttcgatga
     3901 tcatggacta cgaggcggta atagggctcg aaatccacgt acaactgaat tgccggacga
     3961 aaatgttctg ccgctgctcc acccgggtgg gagatgaacc caatcgaaac acctgtccca
     4021 tctgcctgtg gctgcccggc taccttcccc ggttcagccg ggaatccctg gagcgggccg
     4081 ttcttgcctg cctgtctctt cactgcgaaa tccggaagga aagcgctttc gatcagaagg
     4141 tctactacta tccggacctg cccaagggat atcagctctc ccagttccat cagcccctgg
     4201 ccctgaacgg atggctggac attccttgcg gggacggcgc cgccaagaga atccggattc
     4261 ggaagatcca catggaagag gatgtggcca aactggtcca cgagaatgaa ggaaggaccc
     4321 cggtgagtct ggtggatttc aaccgggccg gggtgcccct ggtggaaatc gttaccgagc
     4381 cggacattcg aagtccccgg gaagccatgg aattcctgcg tgccctgcga attcagctgc
     4441 gctattccga gagtgccgaa tgcagcatgg aacagggcac catgcgggtg gatgccaacg
     4501 tttcccttcg ccctcgaggc agcagcgcac tgaacaccaa ggtcgagatc aagaatatga
     4561 actccatccg cagcgtgggc gatgccgttg cttacgagat acagcgccag gagacctgcc
     4621 tggctgcggg cgagcccatt gtgctccata cgcgcctgtg ggaccccgac aacggggcaa
     4681 ccctgcccat gcgggagaaa ttcgaggggc cgtgcgtgcc ggacccttcc gtgcctcgca
     4741 ttgtcatcga ggatgcgtgg ctcgaggact tgaaggcccg cctgcccgaa atgcccgccg
     4801 ggaagaccga tcgtttcgta aaacagtacc agctcagccg ggaggaagcg tcctttctgg
     4861 cctccgaacg ggagctgtcc gattatttcg aggcgacggc ggccctggga gcctccccgc
     4921 gaacgtgcgt tcagtggatc tccggccaac tgctcccgtt gctcaaggaa ggcaatatcg
     4981 ccctcaccga ttgccctgcc gccccggatc gattggcggg actcctggga ctgctggatc
     5041 gggaagaaat caacgccaac gccgccaagg aggtcctcag acggatgatg accgacccct
     5101 ccccgccgga gcggatcgtg gaagaatgcg ggtttcggca ggtttccgcg gagggggaat
     5161 tggaggccct agtggaccgg gtattggcgg aaaatccgtc tgcggtggaa aactatcgta
     5221 aaggggcgac caaggccatg ggatttctta tgggcagggc catgcaggca tccgggggaa
     5281 aagcgaatcc acggctcctc aaggagatcc tggcccggaa gtctgcatga ggcatcgtct
     5341 gccgtgggca ttccggcact cgccccggcg cattggctgc aggcctgacc gcaacgcctc
     5401 aatcccgagg cgaaccccct gaagtctgcc gacctctaat tgtaaacctt gtttctgttg
     5461 gggaggtcgc tctcccattc ctcttcgctc agccgcctgc ggaacaactc gaaagcgtag
     5521 aggagcctcc acaccagcat ttcctgcacc aagttcttga tggcctcccg gggaacgtcc
     5581 cccaaagatt gggcccaaaa gccggccatc acgcgatcca aggccaggat caccctctgg
     5641 tggatttccc gctggattgg ggtcaaggcc cccgaggctt cgttgcccca gacggcatcg
     5701 atcaaatacc ccgaagactc cctcaccaac cgcgaccaat gggcctggaa aacctccata
     5761 gccagttgat ccatgacggt tccgatcctc agctcccctt ccatgcgctt cgctcctgta
     5821 ggatccccgg gcaactttct cgaagctcgg atacgccccc cttccaaccg ggaaaggacc
     5881 gggcggaggg ggcggctcat tgggccgggc cccggactat cctcgatggc agtcgggtcg
     5941 atgggccggc agacgaagct cgaccacggt ccctcttccc aaaacgcttt tcaggctcaa
     6001 cgacccgccg tgacctccaa gaatctcccg ggccacacaa agccccagtc ccatgccgtc
     6061 gggcttactg gtgtagtgcg gctggaatac ctcctccagg cgttcttcgg aaatcccaca
     6121 gcccgtgtcc tcgatggtta cccggatcat ggaggtgctt tcctcctttt ccgcccggat
     6181 tttcaaaagc ttctcggggc ggccctccat ggcttcgacg gcattgagga tcaggttcgt
     6241 aaacacccgt tgcagggcgg acttggtgcc cggcacgttc aactcaccgg gatcgaccat
     6301 gatttgcacc gcaatcccct cccgggatat gaggcctcgg caagctgcaa tgcccatttc
     6361 gatcagggcc cccaccttca ccgtccccaa cgggcacatg atctcgcggg tgaggtccac
     6421 gatgagctgg ttgatcccct ggatcctccg ggtggctccc aagatcttct ccaaccgtcc
     6481 ttgcaccgct ccggtgttat ccggctgaag caggcacagt tctgcgcaac cgacgatggc
     6541 tgccagggaa ttgttgagct ggtgagtgag tgtgggaatg atccccagga gctcccgccc
     6601 tcgattcgac ccgggatttc gtctttcctc ccgcacctct tcgccttttt cttgagcatt
     6661 catcatcaat ttgcctgctt cccgcgggaa cgtttcatcc atttgcccac tgggtcgtcc
     6721 tcgggagagc cgtgcccggt ttgggcgtga atttcctttt ttcgtccggt caaccccgag
     6781 ggtcttcgag atggaatctc gatcaatgga ccttcttttt caatgagaga acaggggcgg
     6841 tatgccgagg cccccatttc atgaacggga cattgggctt tggacggcgg tcattttagg
     6901 caagaaaaat gccaaggtcc tgggcaggcc aaaggccggg ggcgcaaaac agtccgcgaa
     6961 atccggacgg gagcacccgg ggaatcccct tttgcatggg aaatccgtgc cttcggacgc
     7021 ccccgaaaac cgtccgggcc acaaaggcct gcagccgggc ccttctcccg gatctcggtt
     7081 gggcggtgct cgatcccgga ggggggaacg accccggggt cccccctctt ttcagaaacc
     7141 agggggaacg gaagtgaacg gatccccgga atgcgggaat ttgcgacctt cagggatgaa
     7201 aacccgatga ttcgaattct ggaatccggt ttcccggaat cgggaaaaag gacatgggga
     7261 cgggtcctca gaggagggac cgactccgtt cgtagtagtg gctgatgatg tctctcttgg
     7321 agatcatggt gatgatggaa tcgggggctt gccgacagac caccggaagt tcgtccacat
     7381 cgatctggat catcttctgc aaggcggact tcaggctctc atccagaaag acgcacacca
     7441 cgttgggcgt tgccacgtcc ttggccacga tgagatcccc gatgccttct tcgagcatca
     7501 cttgccggat gtcgttcaag gacaagatcc cggtcatctt cccgtctccg ttcaccacgg
     7561 gaaaatgaag ttcctgggag gcggtgacga tcttgaggag ttcatgaaag ggagtactct
     7621 cgcgtatttg ggtcaccggc cgaagggtta cggcctgacg cacttcgagc tgttccagga
     7681 tttgttccgc aaattccccc atgtgagcgg gcgaggtgag tcgcgagccc gtctgttttt
     7741 cgtacaggcc ggtttttccc agggccaggg tggacagggc cgagaccagc atgaggggca
     7801 ccaggagggc gtaattgccg cacatctcgc aggccataat gatggaggag acgggcacct
     7861 tggcaacccc ggcaaaaaaa cccccgatgc ccaccaccac aaaggaagac gggtgcacca
     7921 cgtactgggg cgccagaccg tgccccagga acccgaaggc acctcccaac atggctccca
     7981 tgaacaccga cggtccgaag acgccgccgc tgccccccga actgatggtg caggaggtgg
     8041 ccgcaatctt cataaagacc agcaggagca tggtgcccca gaagatcttg ccttccatgg
     8101 ccgcctggat ccatccgtaa cccccgtcca tgatcggggg gcaaaggagg gccgtcaagc
     8161 ccaggagaaa tcctcccagg gccggtttga gcatcttggg aatccggacg gggcgaaaaa
     8221 cccggtccct ggatccgtaa aagaccttga tgtacacgta accgaccccg gcgcacacca
     8281 tgccgaacag ggcgtaggcc ggcagttcga tcacgctgaa atccacgggg ccggggaaga
     8341 agagggcctg cctgccgaaa tactggtcga aaatggaaga agccacaatg gaggagatga
     8401 tgcagggcag gatggcgtcc gactcgaaat cggtaccccg gtagaggact tccggggcaa
     8461 aaagggccgc tcccagggga gcgtgaaaga tggcgccgat gcctcccgag gcaccggcca
     8521 ggaccaggat gcgccgatcc cggggacgaa ggcgcaggat cgtggccagg accgacccga
     8581 atcccgcccc gatctgggcg atgggtccct ccttgcccgc agaccctcca ctgccgatgg
     8641 tgatggccga agccaggatt ttcaccaggg gcactcgttt tcggatatgg cccccacggc
     8701 ggtggaaggc atcgatcatg gcgtcggtgc cgtgaccttc ggcctcgggg gccgtgaaga
     8761 agaccagcag gcccgccgcc aggccgccca gggtcgggat caccaggaag agccaggaac
     8821 gcaccgacgc cagggcgctc acggcggcag cctccgaccc ctggagaggt tctttgaacc
     8881 cggtgctcaa gaaaaggaag taatgaagcg cctggcgcag gagctcttcg aacagaatgg
     8941 cgccgagtcc ggcgatgacg cccacgacga tgctgagaaa cgtcccccga aaccggcctg
     9001 accacccgaa ttgcttcagt tcggcgattc ttccccgcag cccaagagag attgctttca
     9061 tcgatgccct tcctccatgg acgaccccgc cggggccgtc aagtcccggg gactcaacat
     9121 agaccatcca aaccggtcca acaaggggag tgtcgctcgg gaaggatcgc tccggagttg
     9181 agcgattccg agagcaccct gggctcatgc aggcgaaggg ggcatccaaa cgggggggga
     9241 tgcgcgctct tcccggattt ccgcctgcac gggaatgacg accggcaaag tggcctgcgt
     9301 gagagacggg ttcacggatt cccgcccgag cgggattacc ccatccaacg ggtttcgctt
     9361 cgttccatgg aagtttccgt tttcttatgg aatatggaat gacgaaacgg ggttcgtagc
     9421 ggcccaatcg ctcgatccga gcctccccct ttcccatctt ctttgtcggc aacgagcacc
     9481 cggcgggggt cctcccggcc ggggcgatca ccgttccctc gcctggacac gatcccgagt
     9541 ccaatgctat gatataggct cgggcgcgct tgcggggatc cgatgccaat gccgtcacgg
     9601 ttgctgcttt tgaatacccg cggagggcgc cttcggtttc tttgaatcct ggtttccggg
     9661 caatcatcac tctttgaagg tggacgtatg ggaggtcttt ttggagtcgt atccaaaggg
     9721 gattgtgttt ccgacctgtt ttacggaacc gattatcact cgcacctggg gacccgccgg
     9781 ggagggatgg ccgtccgaaa cgcggaaggc atccagcgat ccatccacaa catcgaaaac
     9841 acctactttc gaaccaagtt cgagccggag ctggcgacct ttcggggaaa ctgcggcatc
     9901 ggcgtcatca gtgacaccga ccctcaaccc ctcatcgtcg ggtcgcacct ggggaccttc
     9961 ggcatcgcca ccgtgggaaa gatcgccaac ctcgaggagc tcatacgaac ggccctgggg
    10021 cggcgccgat ccttttcgga cacctcgagc gggaccatca atcccaccga aatggtggcc
    10081 aagctgatct gcgaggaaga ttgcttcgaa aacggaatcc gcaaggtgca ggccctggtc
    10141 cggggatcct gctcgatgct gctcctggcg ggcgagggaa tctacgcggt tcgggaccgc
    10201 ctgggccgga cccccctggt cctgggccgc cgggacgggg ccgtggccgc gagctcggaa
    10261 tcctgcgcct ttcccaacct gggcttcgaa accgagcgat tcctgggacc cggcgaaggg
    10321 gttttcctaa gtgccgacgg ctgggaaacc cggatcgagc caggaccggc catgcagatc
    10381 tgcgccttcc tgtgggtcta ttacgggtat cccgcttccg aatacgaagg gatcaacgtg
    10441 gaattcgccc ggaatatgtg cggcgccgcc ctggcccgca acgattccgt ccccgtggat
    10501 tacgtggccg ggatccccga ctcgggcatc ggccatgccc tgggatacgc caccgaaagg
    10561 cagcttccct atcggcgccc cttcgtcaag tacaccccca cctggcctcg aagcttcatg
    10621 ccccaaagcc agcaactccg cgacctggtg gccaagatga agctcatccc ggtgcgcaag
    10681 ctgatccaag acaagaggct gctcttctgc gaagactcca tcgttcgagg aacgcagctc
    10741 aaagacaaca tccgggtcct ttacagctac ggggcccggg aagttcacat gaggcccgcc
    10801 tgcccgaccc tgatttatcc atgcgaattt ctgaattttt ccacctcccg ttccaccctc
    10861 gacctggtcg gacgccaggt catccgggag atcgagggca ccgaagacac ggatctcgag
    10921 gcctatgccg cccacgggtc ggaaaaaaac ctggccatga ccgaggaaat tcgccgcaga
    10981 cttcacttga ccagcctcaa ataccagcga ctggaagacc tggtggcgtc catcgggctg
    11041 cccaaggaaa gactgtgcac ccattgctgg gacgggtcca gttacttttg acccccgtgg
    11101 aatcgaggtg cgcagagatc gggagggatc cctgcgaagg cttttgccac cccggggaag
    11161 atctcctccc cggggcctgt ggcggcggag gggccgggga ccgaccgggt tcgtaaatca
    11221 ttgccccgca cctgccatgg agaaccgccc atggaatccg gtccgggaga gggggaaaat
    11281 gcctgcctcc cgggggccgc cggttcggaa acttgcgccg gggacccaga cgaacccttc
    11341 tcccccaaaa agatcccccc ttcttccgtg ggaacgggaa gggggggttg gtggggcgtt
    11401 aaaccgccgg gggccgtgag gcgtcccaaa agaaaaaacg tcgaggcccc gaagacccgg
    11461 ggccgtactc agcgtcagcg agtgggacag cacagggtca gcaccgggct caacccgctg
    11521 ccggaactga aatccccctc gatttttgcc ttggacgcca cactctttgg ctcgtttctc
    11581 agcaggtacc ccagggtccg ggtcttttcg caggccgcca agtccgtatc cagggcgatc
    11641 cagtcgagcc cgtcccgccg gaagcgaacc cctttgcggg tatccagccc gtgcgcctcg
    11701 accactttga tgttgtaagc cctcgccagt tcgaggagcg tctggtagtt cgttcgatgc
    11761 agtgaagatt tttccatgga aacgtcacct cttttccctt ccggtgatga tcggcgcatc
    11821 cggaacggcg cctccctaaa gagtaatcac cgggtcggat ccgtgccacg ggccgggttt
    11881 tcccgtcgga aatccttcca gtagttgacg aaaagcctgc ggcttaccct cgagaaccgg
    11941 tcgaaatccg cggtgagctc catgccggga acgattgcct ttaccacggg gatgcccagg
    12001 tcctcccggg tgagatccac gtacacggcg cgaaagccgt tggcctccag gagcctttcc
    12061 aggacatcga ggttttcctt ccaacttccc cggtcccaat cgggcagctc ctcgaggacc
    12121 cgggtgggaa ggtcttccga ctttcgggtg gcatggggtc ccccgggata gggatgggga
    12181 gtttccatga gagccgacag cagggctttc ctgccgtcga ggtgagcccc cgtgcccttg
    12241 acgatctctc cccgggggcc caccacgaag gatttgtagc acgggattcc cagttccgtg
    12301 gtgagatcct ggaaccggac atgaatgcct cgtttttcgt agtccgccag caggtccgca
    12361 atttcggggt cccgggattc caatcgaaaa cagtgccggg gatgatacgg gatcgtcgcc
    12421 tcggcgtgtc gttcgaggtc ttcgagcaag gcactcaccc gggcctgttc ccggctgttt
    12481 cccgaagcca ggcccgtgga tcccaatccg ctgaaaagcg agatttcatc caggttgcag
    12541 aagagaaaga cacactgaac ggggaccagg acgggccggg tagtcccccc tttccgttct
    12601 tccccttcca tccagtagag ggcttggtcg gcgtaagggg cctccagggg aagctcgttg
    12661 gggtccaacg ctgctcggcc ctgctcccgg aggcggctga ggcggtcgtg gaccagcggg
    12721 tatttcctgc gggtcccgag gaccccttcc ggaccgatgg aggcaaagga agaacagcgt
    12781 tccgccattt ccatggcgta ggaggcccgg gcggcatcgg gcgtcggtcc cttgccgtag
    12841 ctggtctgga ttccggagag gctgtagtgc aggcagccgt tctcgacggt gcgctccatc
    12901 ttccaccttc gaagaaatcc gtgggggctc agcgacgagg tatgccgggt ctcctccccg
    12961 gcgacgacgc ccagggcctg aagcctctcg agagcacgcc gggccgtctc ccccggcggg
    13021 ggatccgcgg gggctttccc gccggagggc cgggcaacac ccccgcagat ttcgcccacg
    13081 tgcacgggag gagaagcgcc cctcgccggc tccgtcggat cgtaaggaaa ggggagaccg
    13141 gcatcgtccg gggacggaag aggttcgtgt tgcgaaagat tccggtcgaa aagctcgatc
    13201 caccgggaat ggagggttcg gtcctcgagg aggctccacc gggcatgatt caacggggtg
    13261 tactcgagca aacgggcgat ttcccggggc tggaaacccg cccccagggg agcgagcgcc
    13321 gggaaggtca gggacgcctc gtagagaagg accgtcacca cgggatctcg gtccccggcc
    13381 atctcgagga tttgacgggc ggtctcgaga tcgagcctgc cgaccatctc caggagatgg
    13441 ttgtgcatga actgatccat ggggcattcc cgaaggtaag ccaggtcctg gtcaaagctc
    13501 gcttccaaag ccggaacggc cgcaaagtat ccgaccccgg aacccgtggt cgtcaaacga
    13561 aggtcatagg gcagaggttt tctcacttcc gcgatccttt ctttccggag tgatcggacc
    13621 atattcgaaa tcgaattcct tgggaagggg agttctggat gcaaatcgca aaatgcggta
    13681 caagatgacc ggggctcccg aaggacccat ggtcgagccc tcccatcctt ccatttccca
    13741 cgggcccttg gcaggccgag aaaggggttg ccatggtcac aagagtcaag tcctgtcttt
    13801 tgctgcttgc cttcttcccc gccttttttc ccttttcggc aggggggacc ccgtcggaag
    13861 acgaggacgt tacggcccgc cggggaaagc ccctgggggg acagggtacg gagatgccgg
    13921 tcctgacgct ctcgaagccc gaaggcggct ggacgtcggc catgcagatc gaggtggcgg
    13981 gcacctgttc cgaccccacg gccgatcccg tcaccgtcac cctcaacggc gttcgctact
    14041 tcgtgcgatc cacccagggc tcgtttgcga ggaaattccc cgccgccaag ggcaggaaca
    14101 ccctcatcgc cgaatgctcg aaccgggccg gaaccgcccg ggccaccgcc gaggtggaag
    14161 ccgtgatccc gcccattccc ctgaaggtgg tgctcaccaa cgacaccgat tccgcctaca
    14221 cggacctgca cgtctacgag ccggataaga gccacgtgta ctgggcaaag acccattccc
    14281 cctcgggggc cgtctttttc ctcaaccagc aggacggctc cttcgaccag gcgggatacg
    14341 gcccttacct atacgtgcac ccggcaccgc ccccgggcgt cttccgggtg gacgccaact
    14401 actggccggg aggcgccgtc cggcacaccc tggccaacct ggacatcgtc accgacgaag
    14461 ggcttcccac cgagagccgc cgaagggtga gaactcccct ggcccgaccc ggtgaaaccc
    14521 agaccctggc ctacatcgtc attcgaggaa acaaccagcc tcccctcgtg tacgtgcccg
    14581 gccaggaccc tccctcggac atgcctcccg aagtgaagga gtaccaagaa aagggagaac
    14641 cccgggagtc ctcggacgaa gagtcggcgt tcctgcaccc cccggacgaa aaatccttgc
    14701 gggaatccgt gtcgcggctg gctttgctcc aggcccggaa aatcagtccc aactgggaga
    14761 agaaacaacg ggactgctcg gggctcgtgc gcttcgcgtt ccgggaggcc ttggaacccc
    14821 gcacccgccg acagggagaa gaacacggaa tccccggggc actgcatctc ccggcactgt
    14881 ccccggcatc gcggaagctt tttccccact atcccgagat ctggcagatc ggctacaccc
    14941 gggaaggaac cccgtttttc gggtcctttg ccgacgccga aacccttctg ggcttcaact
    15001 ttcgaaggaa gggccgagac ctgggccttg cccgcaacgc cgacctgctg gtgtttcgaa
    15061 aggcctacga gagtgaccaa ccctaccacc tgatgatttt tgtggaaaac cgccccgatg
    15121 ccctggcggt ctaccacaac ggagcttctg gagccgaggc ccaggtccgg gtcgtgcggg
    15181 tgggggatct catggaatcc ccggaccccg tctggcttcc caccatgaac aacccccatt
    15241 tcttaggagt ctacgagtgg aaccgactaa gacccaaaga cccaaccgca tcctgaccgc
    15301 cgccgtgctg gggaccttgg cggcaggcct ggtcttttac tgggcgacct cgcggctcca
    15361 ggccacccgc ctgcatcaaa tggtgcgaag cgacctgccc gtcctggatc tcgagctcga
    15421 attcatcgat gcccaggggg agaaggtccc acccgaccgg acccggctgg gaggaaagcc
    15481 cccggagccc agggcaaaaa ctcccgaacc ttcccccgag gaatgccccg agctggtggg
    15541 gggagaccga acctggggtc ttcaaccttc cctggacgag aaaaggcccg accattgtcc
    15601 caagacggca cgatggaagg tgaggcgaca tcccgtggcc ttcagcctgt atttcctgga
    15661 cgggccccgg cttctctctt tcctggaaaa cgatccccgg gccaaggagc tggtgggttc
    15721 caagttggtc cagggcctgt tttacgatcc cattcatgcc gggagagttc gagcccggga
    15781 cctgcagctc gagggcatcg agggcgccgt gctgtcgacg cttgttcgag aggccctggc
    15841 ggcccgcgca gagcttcatt acgacatgtc ccacggtagg aaggggtttg ttttctcctt
    15901 cgtgcggaac gagtgcccat tcgcctcgag gctgttgccc ctcatcgggg caagattggc
    15961 ccgaagcggc tatcgaatcc cctccctcaa agaacccgtc ctggagatga gaatcggccc
    16021 tcagcgcctc tttctcaccc aggtcgagga ccgggtctac cttgccaaca gcctcgaggg
    16081 actcctcaac gtcctcgaga gcctcccgcc tcgacccgtg gaccttccca aaacccccct
    16141 ggtcctcacc atgcggggag aggccttcct cgagaaggtc ctgcccgtcc tcttcgggtc
    16201 cgagacctgg caggcacacc tgggcatcgg gctgtccgca gaagacccgg gggtcgtcga
    16261 gtttcccctg ggcaaggcgg cccggcacct gagcccgcgg attttcaagg gagtcctggc
    16321 cgggattccc cgggacgttt tcgcctccgc ggcggccgga tttcacatcc cgcccgacat
    16381 gaccccggag caatggcggc gcctggccgt cgagggcccc ggagagccgt cgggaaacct
    16441 ccccggggaa gcgggggttg ccctggtgtg ggacctttcg tcccaggacg gccccatcac
    16501 ccaggtgggc ctggccgttg ccgcccagac cgctccggac aaggccgagg gattcaagcg
    16561 atacctcgcc gatcccgaac tcacggccgt gtgcggcgga ggcaccgttt tcctggccgc
    16621 ctcttcgcga aacctcctgg tgcgtatgaa ggaatcctgt gaagcccagt ccctgagcgt
    16681 tctcgattgg gaccggggac ccgttcggga tgagctcgaa aaggcccagg tcttcgtttc
    16741 cctgaatccc ggcgcggggt tgaaggagct cttgctggcc gggggagcca aggccgggga
    16801 taccggggac ttcgcccccg aatggaaaca gcaatacgaa aaggccaagg aagccatgcg
    16861 gttcgagggg gaatgggtgt cccgggcact tcccgtcctg gcctacgccg ggagcggagg
    16921 cctcccgaat gaaccggcgc gattgaagca agtaagagtc cgagaaggag ggtcccgatg
    16981 aacgctcgcc cttgggtcac cgcaatgtcg gcagccttgc tggtttccct gtgccttgcc
    17041 ctgcccgtag ctcaatccgg gagcccgttc tatctcaccg tcgagaggag cttctcgaac
    17101 accgaaaagc cccggttgcg gctggactac accgatccgg cccggcccat tcttcttcga
    17161 gtcctccggc cccaaaacgt ggagcgcttc ctggacggtc agctgcacat cagccgcagc
    17221 tacgagcagc ccctctcgga gctgaatccc ggccattacg tggttcgagg catcaaccgg
    17281 gcggagtcgc ctctgcgggg gcttcgggac atgctggacc cgggcttcag aaaaaccctc
    17341 cagggaacct cgttcaaccg ggccatcgtg gaaacgacct cgggggagct cgcctcgccg
    17401 ccccagcagg tcattcacaa tgccccggcc gggttttcca cggtccggga agactacctg
    17461 gacctccagt tcgggggaaa aggaacccgg gacctggggt ggtggttcgg ggattccgcc
    17521 ttgcgggagg atcaatacaa gattcgctgg atcgaattgg accccctgtc cgacggtctt
    17581 tacctggttc aggcggttca aggaaaagcc gaagcccagt gcctgctgca ggtgagcagc
    17641 ttgtccgtcc aggtcaagca gtcttcggag caactggccg ttcgcatcgt cacccgcacc
    17701 caggagcccg tagccgaggc ggcggtgagc tatcgtgacg gccgggggaa atggatcttt
    17761 ctggaccgaa agaccgacgc ccggggagag gtggtcttca ccagccccgg cggcgccttg
    17821 gacggaaagc tcctcgtgcg ggcggaaacc gccgacggtc gccgggcctt tgcggatacc
    17881 gattttctcc cggcggtctc ccgggacgat tcggtgttta tcgtgacgga ccggcccatc
    17941 ttcaagcccg gcgagacgtt ttcctacaaa ggggtggtcc gttccttcga cgagggggag
    18001 ctgaagatcc cggaattccg tcagaaagac gcccggatct ccctcatccg ggccgacggc
    18061 aaggccacgg acctggcctc gacggtccct ctctccggtt tcggctcttt tagcggaagc
    18121 ttcgacctgg acgaggcgga gactcccggt ctctatcggc tggtggccga aatcgacggc
    18181 aagccctacg gaggggaatt ccgggtccgg gactacgtga agcccacctt ctacctcga
//
LOCUS       DAOOVC010000070        18136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2104_length_18136_cov_7.179636, whole genome shotgun sequence.
ACCESSION   DAOOVC010000070 DAOOVC010000000
VERSION     DAOOVC010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18136
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_18136_cov_7.179636"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLM79_10245"
     CDS             complement(<1..1129)
                     /locus_tag="PLM79_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86732.1"
                     /translation="MHAGKKSRLKVAYAALILSLSFLGTGGTCAAYPVHFTDSSGSPI
                     VVSERPGRVVSLVPEITGILFTLGAGEAVKGLTRHDTLPPETDGGKVVGGFPSPSVEK
                     IEALQPDVVFLSSVHREVRHRLAGGPCRLIELRCGSIEDIVQTVETLGDIFDKREKAR
                     EVIRTIRDKLQLVSRKLERIPPGQRKRVLRIMGRDRLMTPGNDSFQNDIIRAAGGIPP
                     ELGKQGEAVEISLEEWRRFDPQVIYGCGGDRETAERLLTRPGWKDVEAVREGRIYTFP
                     CGLTCRPSLHSADFVAWLASAVYDEPFSINANQVLEEKRTGSKPVELPLDFVKSAQVV
                     DSTLYDFPNKTLVVEFKEPMRVLSTLEGERSGITCVGNHGSP"
     gene            1210..1539
                     /locus_tag="PLM79_10250"
     CDS             1210..1539
                     /locus_tag="PLM79_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86733.1"
                     /translation="MWQLRDPFIVSGFELRALNGSASQLEGSTVKKKHRHGNPRFPSF
                     KTRTKVLGIESPQAVGFSPVHVRNQRRFFPNVTRLPEKYTKVFAVPVPEACVMPSRVN
                     PPGRFDP"
     gene            complement(1674..2855)
                     /locus_tag="PLM79_10255"
     CDS             complement(1674..2855)
                     /locus_tag="PLM79_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86734.1"
                     /translation="MRRWREVYAVFLGLGILLATGLPVGAVDEAVIATVAADYSSGAH
                     SVASVDPVGGPRTIRNKLAPTISDITLASHGRYFYRIERYNADNITKFNIKNPGAPIW
                     QFSTTDPGDVESSNPQDMVFASGTKAYVLRYGSTKAWVVNPSAKEESQFKVGRLNLGA
                     YADSDGLPEMTGGVVANGKLFIVMQRLDRDHDYVPSNIPYVAVFDIKTDKEVNTGKGS
                     NGLKGIPLPIRNPMSIQYLKQNNTVYVQGIGAWPGSSDPKYEYTGGIVSINPETYETK
                     MVLDDGNKSKHPYGAISGMAIVSKDKGYFVGYMGWGDNVLFAFNPTTGKVLGEVGQGL
                     SHVNITGFESSGMKVDKNKMLWVCDATNARVVILNTATDKVDERLKTRLNPTKVVFVR
                     H"
     gene            complement(3036..4970)
                     /locus_tag="PLM79_10260"
     CDS             complement(3036..4970)
                     /locus_tag="PLM79_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV86735.1"
                     /translation="MESIVVTAQRPEMSRETGDVNLDSTPSFYSLITRKEFEGKIESL
                     AEVIEKETGVQVRQAGGLGSFSTVSLRGSATDQVLVFLDGILLNDASGGGVDLSTISL
                     SDVESIEIYRGATPVSFGKSSIGGVVNIRTLRAQKGIHGNLAAGYGSFDTRKLSGFLS
                     HKPGRWDYLVSADYQASGNDFKFLNDRGTSWNPKDDREERRHNAQFRENSFLGKIGYD
                     FTEDARLDFMDQWFSKDQGLPTWNNSKLAKTSFDTERNIATVKLTANDVGPYHFNTST
                     FLDFMHKVEEYDDSEGQVGLGRQRNRYTTNRFGGHCLIDRHTDRNTLSLMLDAQRETY
                     APEDLLNSKNDLYESSRNSFSIGLQDSLYLFRERVILTPAVRYTHLFDERNGAVNLWR
                     SSDKGSNGDEGSWNPQVGFKVRPRDDLTLKTNIGRYVREPSFFELFGDRGFFIGNPDL
                     KSERGTNFDVGFEYCWPGNHRWVRNVVLSAAYFHNDVSDLITRVYDARGIGKSVNISG
                     SLIRGVETSLKIELLSHFRLIGNYTRQDPIQESEIEAFDGKVLPGRFQESYFGRFEAF
                     YGPVKVYAEHKTEKGLFYDTANLLGAPNKKELNVGASLVLRSFTFTLEARNVGNEQYE
                     DFNGYPMPGRSAYLTVQYKF"
     regulatory      complement(5217..5413)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5404..6300)
                     /locus_tag="PLM79_10265"
     CDS             complement(5404..6300)
                     /locus_tag="PLM79_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HOV86736.1"
                     /translation="MKEKLPKRWMQASVWILCLLLSASTGFAAHGEKATPKKAILLAA
                     FGTTVPEAQKAFENVEARVRQVFPGIEVRWAYTSSIVRAKLAKQGKVLLSPESALAKL
                     MDEGYTHVAVLSLHTIPGVEFHELNQNARLFGQMAGGFEKILVARPLLSSHEDLERAA
                     KAMVKSMPAGRKPGDGVILMGHGTENHPSDAVYLALYHLLQETDSNVHIATVDGYPAF
                     RDILPKLKEKKVKKVYLVPFMAVAGDHARNDMAGDGPDSWKSLLGKEGFTVEPVLRGT
                     AEIPEIVDLWVDHLKVVFDHFQ"
     gene            complement(6363..6620)
                     /locus_tag="PLM79_10270"
     CDS             complement(6363..6620)
                     /locus_tag="PLM79_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86737.1"
                     /translation="MRKGMILYVTEGKEDVPLQTGEEILETARSLGVTAVCVASSEED
                     AVHGWYGLVTKGVQQVLFMTVSYNAQVDRFESRGNPVRLCG"
     regulatory      complement(6796..6981)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7343..7654)
                     /locus_tag="PLM79_10275"
     CDS             complement(7343..7654)
                     /locus_tag="PLM79_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86738.1"
                     /translation="MEIWISGQRREPTLVSDISSMTEDELQAEVNTLAKWGIIFGFGW
                     LLGLGSLVAILFGFRASRIVWGSKGRIRGKGKAIFCVILGVVGLLPWIWRGYVALPLP
                     L"
     gene            7910..8062
                     /locus_tag="PLM79_10280"
     CDS             7910..8062
                     /locus_tag="PLM79_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86739.1"
                     /translation="MNTMKKIGTGMLMVLILGGMLSSSFALDKGGGWEDQAKSYERDR
                     GFIVGD"
     gene            8138..8287
                     /locus_tag="PLM79_10285"
     CDS             8138..8287
                     /locus_tag="PLM79_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86740.1"
                     /translation="MNAIRTIAAALVMALVLGSAGTGLAALDRGATVYTPGAFDSNIA
                     QPNDD"
     gene            8602..9177
                     /locus_tag="PLM79_10290"
     CDS             8602..9177
                     /locus_tag="PLM79_10290"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOV86741.1"
                     /translation="MSPRWNMRVVALLICLVVFLPCFSVHGSQDEQFPPGAQLAAFNL
                     PGPDSAQVQAYLGLKTRDPFTISKIGAKLVYIEFLGAMCPQCHVNAPVVNKLYRVIQD
                     DPALARDVKIIGIAVGNNRAQIEAYKKNFKVAFPIFPDEDLSISAAVGGADTPTMILV
                     TNSGKILSSHRGVITDFDGYLKQLREFHKKQ"
     gene            9184..10107
                     /locus_tag="PLM79_10295"
     CDS             9184..10107
                     /locus_tag="PLM79_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOV86742.1"
                     /translation="MAENENKGNRLKESTIFRGLPQEKLDEVARAVQNVVVPGNSLIF
                     REGDPGDSFYIISSGRVRVFRRLGGDMETELSVLGPGDSFGEMALLTGEPRSADIETL
                     EETHLMVLTKDQFDHILRDCPDISRTFVKEMRRWLLRDQELLEVEAREAYKATRLSWV
                     DFLVVFAVSIVLALIFNSSNPNGIPLFPKFPDRASFPSVSPVEAMEAVQRGDALLVDA
                     MPSNFFAKRHIQGAVNMPLALFDIVYMMSFAEEDKEKKIIVYGGTVSKLYDLEVANKL
                     LLRGYSNVRILEGGLSAWEQNGYPVVEKAKK"
     gene            10104..10607
                     /locus_tag="PLM79_10300"
     CDS             10104..10607
                     /locus_tag="PLM79_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOV86743.1"
                     /translation="MMGAFIKRIVTSEYLAFVLRIYVGYFFIYASMSKIPYPAQFAEA
                     TANYRIIPYWLLNSGAVFLPWIEFVAGLFLIIGFMSRASVVLIGCMLVMFNVMVLINM
                     YWGAPISCGCYDTVGEPIGWKKILENAVMLVFAVQIFFYDRLFIFRRGGFSRAARKRV
                     PSPAGAR"
     gene            10616..10960
                     /locus_tag="PLM79_10305"
     CDS             10616..10960
                     /locus_tag="PLM79_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86744.1"
                     /translation="MLNISRSHSVLWLLPLFLVLVLVLSCGSDNQMVGTYRAETKESA
                     KQAEVLVELKANGEGTWKMGEEEVPFSWYLKRGELRVNTKGGGVIVGTIDKDVIHMTL
                     PGTGGLSFKKVQ"
     gene            11187..16697
                     /locus_tag="PLM79_10310"
     CDS             11187..16697
                     /locus_tag="PLM79_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86745.1"
                     /translation="MRLRLGHRILVMYVLFGACFSLFTGTLVFSRLKEGRFTKVGSQF
                     QHQLNHIDLALTLYFDEAEADLGGLAGDEFVRSLTGEDSSPGARTDPRVFPYREPDVK
                     RRVLHLFRSFKDAHKHVTSLYLAWEGGGYLEYPSRERPPGFDPREVSWYGSAKAAAGK
                     VVRTDPYISHASSAILISMTAALVDENGRFLGVVGMDVNFADLKGYLKGVRAGQTGQL
                     VLVDQHGILLADPDLAAFSRPISSVYAGDLAPLFERQAGFVSVSRALQRDYLFFTTSK
                     KMGWKLGLLVPQNEIDREIESFVYGIVAAVLLSIAVLTVLSLLGLRHFVLLPIRNLHR
                     GTELIARTGDLGHRFEVRSPDEIGDLAASFNAMIETLREAKEALKESQGELKRHRDHL
                     EELVEARTVDLKKLSEALKQSPASVIITDQQGNIEYVNARFCEVTGYSLEETLGKNPR
                     ILKSGKHPPSLYRDLWETILAGKTWRGELINRKKNGEEFWESASISPIMDDRGRITHF
                     VAVKEDITEQKAVEARLQLTQMAVDHIFEQTLLVDRDGRLCMVNEAASRVLGYTREEL
                     LDMSVWDLFEGFPPGSWPEKWQEVKAAGSLVFESYHRTRDGHTYPAEVTASFFRYEGQ
                     EYVHAVSRDVTERKRAQEELAEAEERSRLLLESAGEGIVGVDPRGKVTFVNPAATLML
                     GYLPEDLIGRGLHQLIHHSYADGAPYPAENCPMFKSYSYGIVNEVEDEVLWRKDGTCF
                     NVEYSSTPVWKNDTLMGAVVTFRDVTERKRMEAALREREEYFRALFDHAGVGIVSTDN
                     EGRFTRVNEQFLEFIGYTWEELKTLPLVALCHPDYREKAKEAMERLRNGQIDLIRTEM
                     LYVRKDGVPRWADVRSGPIRDAEGNLIASVTTITDVTERKRSEVEQARRLRTEKAMAA
                     VSRALLGSDTESETLQDALKQIVAAAQVDRVYVYQNVEDPAEGLSMWLIFEACAPGVE
                     SISQNREMVQRPYARGLARWQETLGQGKPIMGPVNSFPNEEQEFLRGHESLSTLLLPI
                     QVKKEWFGFIGLDDNHLRRNWTSSDVTLLGSTAEIIGAFLGRQRAEEELRIAKDKAEE
                     ATKAKSVFLANMSHEVRTPMNAILGMTYLALQTRLTPKQKDYLAKIQRSTKSLLGIIN
                     DILDFSKIEAGKLQMEDTDFSLEDVLENVSAVVGIKAHEKELEFLMDTAANVPTALHG
                     DPLRLGQVLINLCNNAVKFTDRGEIVVSARVAERGEDWVVLQFSVRDTGIGLSEVEKE
                     RLFLAFSQADTSITRKYGGTGLGLTISRRLVQMMGGKIRVESVPGQGSEFIFTARFGL
                     SGKGAARTLEPLPDLRGIRVLVVDDNPSAREIFQNLLESMSFEVSLAASAEEALWEIE
                     KEAGGRPYELVVMDWKMPGMDGIKASEAIRRHPGLPRQPRILLVTAYGREEIMTRAEK
                     AGLDGFLVKPVTQSILFDAIMDAFGRRDRREDPGGRSAREAAESFHRIRGAKVLVAED
                     NEINQQVAVEVLQRAGLRVRVVDTGSRAVEKVLEEPFDAVLMDIQMPEMGGFEAARIL
                     RKDPRFRDLPIIAITAHAMAGDREKSLLAGMTDHVTKPIDPDELLCVLARWIRPREGE
                     FPECEPARESAEEGPTDFLPENLPGISLEAGLKRVGGDRALYGKLLARFRRSQADTPD
                     KVRTALEQEETRTALGLLHTLKGVAGTLGAEGLAVAASRLEEAVNAGHPGDIDPCLRT
                     LAERFQEVFEGLEEWEGAESRTQPPIRPATEDSMDRDRVRRLVEETGRLLETDLPEAI
                     LRFESLKDHLPRSSFEEEIKGLESSLEGFDTDAALGFLAVIAAGLDRPREGE"
     gene            16701..18053
                     /locus_tag="PLM79_10315"
     CDS             16701..18053
                     /locus_tag="PLM79_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV86746.1"
                     /translation="MAEGSPKPKVLVVDDTPENIQVLMGTLRGDYAVVAATNGEKALR
                     LAAAQPPPDVILLDIMMPGMDGFEVCRRLKAVPETRDIPVLFLSALDEVSHKIRGFSI
                     GAVDYISKPFQPEEVHVRVNTHLTINRLRRKLEEKRRELEVYNESLEERVRARTAELA
                     ALNEVYERFVPREFIDLLKKSSILEVRLGDQVKQRITVMFADIHGWTGLLERMSPPDA
                     FRFFNDYLSRVSPEIKACRGFIDQYYGDGIMALFPGPPDDAVRAAVSMQTAVFEYNRA
                     REQDGLEPIQVGIGLHFADLMLGIIGNRDRMQGAVVGDDVNLASRIEGMTRMHGARIA
                     LSEQTLLQIQDPTRFKHRWIDKVRVKGRREPVTVYEVFEGDPPPEARLKESTRTQFEA
                     GLRLYYGRKFSPASVQFNGVLEKNPEDLASRIYLERCARYMVNAPPDDWTGVESLEEK
                     "
BASE COUNT         3664 a         5432 c         5345 g         3695 t
ORIGIN      
        1 gcggtgagcc gtggttgccg acgcacgtga taccgctgcg ctccccctca agggtcgaca
       61 ggacccgcat gggctccttg aattcaacga cgagggtctt attgggaaag tcgtagaggg
      121 tggaatccac tacctgagcg gatttcacga agtccagggg aagctcgacg ggctttgagc
      181 cggtgcgctt ttcctccaat acctggttgg cattgatgct gaatggttcg tcatagacgg
      241 ccgaagccag ccacgcaacg aaatcggcac tgtgcaggga cggccgacag gtgagcccgc
      301 aggggaaggt gtagatcctt ccttcacgga cggcctccac gtccttccaa ccgggtcggg
      361 tgaggagcct ctccgctgtc tcccggtcgc ccccgcatcc gtaaatcact tgcgggtcaa
      421 aacgcctcca ttcctccagt gagatctcga cggcttcacc ctgcttgccg agctccggcg
      481 ggattcctcc ggcggctcgt atgatgtcgt tctgaaagga atcgttgccg ggagtcatca
      541 gccggtcccg gcccatgatc cggaggaccc tcttacgctg ccccggcggt attctttcca
      601 gcttccgtga cacgagctgc agcttgtccc tgatcgtgcg gatcacctca cgggcctttt
      661 cccgcttatc gaagatgtct cccagtgtct cgacggtttg aacgatgtcc tcaatggaac
      721 cacaccgcag ctcgatcaac cggcaagggc cccctgcaag gcgatggcgc acctcgcggt
      781 gcacggacga gaggaagacc acgtcgggct gaagtgcctc gatcttctcg acggagggag
      841 acggaaaacc gccgacgacc ttccctccat cggtctcggg agggagtgtg tcatgccggg
      901 tcaggccctt tacggcctct cccgctccca gcgtgaagag gatcccggta atttccggaa
      961 ccagggagac gacccttccc ggtcgctccg acacaacgat tggcgaaccg ctggaatcgg
     1021 tgaaatgtac ggggtaagcg gcacaggtcc cgcccgttcc caagaatgac agtgacaaaa
     1081 tcagggcagc ataggcaacc ttgagccgtg atttctttcc agcgtgcatc taccatttcc
     1141 ttccttcttg gtgccactcc gaaaccaacc cgcgctgcag cgatccctcc gagtgccgac
     1201 caaggcacga tgtggcaatt acgtgaccct ttcatcgtgt cgggcttcga gttgcgggcc
     1261 ctgaacggaa gcgcatcgca actcgaaggg agcacggtca aaaagaagca ccgtcatggg
     1321 aatccgcgat tcccatcatt caagacccgc accaaggtct tgggaatcga atctccgcaa
     1381 gcggtggggt tttccccggt ccatgtccgg aatcagcgcc gtttcttccc gaacgtcacg
     1441 cgccttcccg agaagtacac caaggtcttc gccgttccgg tacccgaggc gtgcgtgatg
     1501 ccctcccgag tcaatccgcc gggaagattc gacccctgaa gatgactcaa tcggcccatg
     1561 gagcgccttg tgtctccgag aattcgggtc aggggctgcc aggcgaggaa gccttcatgg
     1621 ctctcgatcg tttgcgtcat ttcccgtctt tcatgtcgga tcctctcgag gcgtcaatgc
     1681 ctcacaaata ccaccttggt cggattgagg cgggttttga gcctttcgtc caccttgtcc
     1741 gtggccgtat tcaagatcac cactcttgcg ttggtcgcat cgcataccca gagcattttg
     1801 ttcttatcca ccttcattcc cgaagattcg aagccggtga tgttcacgtg gctcaagccc
     1861 tgcccaactt cccccaggac ctttcccgtg gtcgggttga aggcaaagag tacgttgtcc
     1921 ccccatccca tgtatccgac aaaataccct ttgtccttgg agactatggc cattcccgag
     1981 atggcgccgt acggatgttt cgatttgttt ccatcatcca gcaccatttt ggtttcatag
     2041 gtttcgggat tgatcgagac gattcctccc gtgtattcgt actttggatc actcgatccc
     2101 ggccaggcgc cgattccctg cacataaacg gtgttgtttt gcttcagata ctggatggac
     2161 atggggttcc tgatgggaag gggaatcccc ttgagtccgt tcgaaccctt tcccgtattg
     2221 acttctttgt cggtcttgat atcgaaaaca gcgacgtaag ggatattgga agggacatag
     2281 tcatggtccc ggtccagacg ctgcatcaca atgaagagtt tcccatttgc gacgacaccg
     2341 cccgtcatct ccggaaggcc gtctgaatcc gcataggcgc ccagattgag ccgccccacc
     2401 ttgaactggg attcctcttt tgcactggga ttgaccaccc aggctttggt cgaaccgtag
     2461 cgcagaacgt aagcctttgt ccccgaggca aaaaccatgt cctgggggtt gcttgattcc
     2521 acatcccccg ggtccgtggt ggagaattgc caaatgggag ctccgggatt cttaatgttg
     2581 aatttggtga tgttgtcggc attgtaccgt tcaatgcggt agaaatacct tccatgagac
     2641 gccagggtaa tgtccgaaat cgtcggagcc agcttgtttc gaatggtgcg cggccctccc
     2701 acggggtcga ccgaagctac ggaatgggcc ccgctcgaat agtccgccgc aaccgtggcg
     2761 atgaccgcct catcgaccgc accgacagga agcccggtcg ccaacaggat gcccaatccc
     2821 agaaacaccg cgtacacctc tctccatctc ctcattcccg tcctcctttc ttctacaagc
     2881 gcttgacagg acactcacca cggggcaggc catttcccgg cactgatcgt gatccgcatg
     2941 gatccgccgc ccccttcaca ctcgcgccac gtggcgcaaa gcgggaccga ccgccgatgc
     3001 tcgttcatat cccggcgagc cgtcggcggt tcgaactaaa acttgtactg caccgtcagg
     3061 taggctgatc ttcccggcat gggatagccg ttgaaatcct cgtactgctc gttcccgacg
     3121 ttccttgcct cgagagtgaa cgtgaacgac cgaagcacaa gggacgcgcc tacgttgagt
     3181 tccttcttat tgggagcgcc caggagattg gcggtgtcat agaagaggcc tttttcggtc
     3241 ttgtgttcgg catacacttt gaccggcccg tagaaggctt cgaaccttcc gaagtacgat
     3301 tcctgaaatc gccccggcag gactttgccg tcaaaggcct cgatttcact ttcttggatg
     3361 gggtcttgcc gggtgtaatt gccgatgagg cgaaagtgac tcaacagctc gatcttgagg
     3421 gaggtttcca cgcccctgat gagcgagccg gagatgttca ccgacttgcc gatgccccgg
     3481 gcgtcgtaga cgcgggtgat caggtccgac acgtcgttgt ggaagtaggc cgcactcaga
     3541 acgacattgc gcacccatcg gtggttaccc ggccaacagt attcgaaacc cacgtcgaag
     3601 tttgtccccc tttcggactt caggtccgga ttgccgataa agaacccccg atcgccgaaa
     3661 agctcgaaaa aagaaggctc ccgaacatat cgcccgatgt tggtctttaa ggtcaggtca
     3721 tcccggggcc gaaccttgaa ccccacttgg gggttccagg agccttcgtc cccattggag
     3781 cccttgtcgg agctcctcca aagattgacg gcaccgttgc gttcgtcgaa gagatgcgtg
     3841 tagcggacgg caggggtcaa aatcaccctt tcccggaaca ggtagagaga gtcctgtagg
     3901 ccgatggaga aggaatttcg ggagctttcg taaaggtcgt tcttcgagtt gaggaggtcc
     3961 tcgggagcgt aggtctccct ttgagcatcc agcatgaggc tcagggtatt ccggtcggtg
     4021 tgccggtcga tgaggcaatg gccgccgaaa cggttcgtcg tatagcggtt gcgctgccta
     4081 ccaaggccga cctgtccctc tgaatcgtca tactcctcga ccttatgcat gaaatccagg
     4141 aaagtgcttg tgttgaagtg gtatgggccg acatcgtttg cggtgagctt cacggtcgcg
     4201 atgtttcttt ccgtatcgaa gctcgttttt gccaatttgc tgttattcca ggtgggcagt
     4261 ccctggtcct tggagaacca ctggtccatg aaatccagac gggcatcttc ggtgaaatcg
     4321 tagccgattt tgcccagaaa gctgttttcg cggaattgtg cgttatgccg cctttcctcc
     4381 cggtcatctt tcggattcca ggaggtcccc cggtcgttca agaacttgaa atcgttgccg
     4441 ctggcctggt aatcggcgga aaccaggtag tcccacctgc cgggtttgtg actgagaaac
     4501 cctgagagct tcctcgtgtc gaaggagccg tagcctgcag ccaggttgcc gtgaatgcct
     4561 ttttgagcgc gcaaggtgcg aatgttgacc accccgccga tggaggactt gccgaaagag
     4621 accggggtgg cgcccctgta gatctcgatg gactcgacat cggagaggga aatcgtgctc
     4681 aaatccaccc ccccgcccga ggcatcgttt aggaggatcc cgtccaggaa caccaaaacc
     4741 tggtcagtcg cggaccctct tagagaaacc gtcgagaaac tgcccagccc gccggcctgc
     4801 cgcacctgga caccggtctc cttttcaatg acctccgcta gactctcgat ctttccctcg
     4861 aactccttcc tcgtgatcag ggagtagaat gaaggggttg aatcaaggtt gacatcgccg
     4921 gtctcccggc tcatttcagg cctttgagcc gtcacgacga tcgattccat ggaagccgtc
     4981 gcctggtcgg tctgggcatc ggacaagggc ggacaggcga caaagagcag aagcaccgcc
     5041 gcgagagggc ccgcccaaag cttcggcttt ctacccgact gccgacgacg gggggaccta
     5101 tgaggagtgc cgggacaagc ggttccaaag aagccggggc ctttgatgca acccgtgacg
     5161 agcgatgaag gcaggcattt caaagatggg cctcctcttc ccccctcggg gatgtgcgag
     5221 aggttcgaac atcgttcggg tatcctggct cacgttcgtc ctactcaccg cgccttccca
     5281 tcgcgctctc ggcttgacag tggcgtgcgt gcggctttcg tcccgatcac agtcgcgggg
     5341 cggcggaaga ttcgcacttc cttccctgaa tcgatgcggt ctcgttgatc ttaaaagatc
     5401 tatctattgg aaatggtcga acaccacttt caggtggtct acccagagat cgacaatttc
     5461 cgggatttcg gccgtccccc tgagcaccgg ctcgaccgtg aatccctctt tgcccaggag
     5521 ggacttccag ctgtcgggtc cgtccccggc catgtcgttg cgcgcatggt ctcccgccac
     5581 cgccatgaaa ggcaccaggt agaccttctt caccttcttc tccttgagct tcggtagaat
     5641 gtcgcggaag gccggatagc cgtccacggt cgcgatgtga acgttggaat ccgtctcctg
     5701 caagaggtgg tacagggcaa ggtacacggc gtcggaagga tgattctcgg tgccgtgccc
     5761 catgagtatg acgccgtctc ccggtttcct gcctgccggc atgctcttga ccatggcttt
     5821 ggccgcccgc tccaggtcct cgtgggaaga aagcaggggc cgggcgacca ggatcttctc
     5881 gaagcctccg gccatctgtc cgaaaaggcg ggcgttttgg ttcagctcat ggaattccac
     5941 gccggggatg gtgtgcaggg agagtacggc cacatgggtg tagccttcgt ccatgagctt
     6001 tgccagggcc gattcgggag aaaggagcac ctttccctgc ttggccagct tggcgcgtac
     6061 gatgctcgag gtataagccc agcggacttc gatacccgga aagacctgcc tgaccctggc
     6121 ttcgacgttc tcgaaggctt tttgagcctc aggaacagtg gtcccaaaag cagcaagcag
     6181 gatcgccttc ttgggcgttg ccttttcgcc gtgtgcggca aagcctgtgg aggccgacag
     6241 aagcaaacag agaatccaaa cagaagcctg catccatctc tttggcaact tctctttcat
     6301 cggttcatct cctctcttga agtctgggtt tcaggctgca aaaccttcga aatcccggct
     6361 gatcatccgc atagccgcac gggatttcct cgggactcga acctgtcgac ttgggcgttg
     6421 taggaaaccg tcatgaacag gacctgctgc acccccttgg tgaccaggcc gtaccaaccg
     6481 tgcacggcat cctcctcgga acttgccacg caaacggcgg tgactcccaa agaccgggcg
     6541 gtctccagga tttcctcccc cgtttgcaga gggacatcct cttttccctc cgtcacgtac
     6601 aggatcatgc ccttcctcat tgaaccacct cctcccgagc ggtttgcttc ggagccgggg
     6661 tcggccccgg accagaaagc ttgttttgga caacaggacc cttggcaaaa aaaaatcctt
     6721 agagctgaaa gctctaagga ttgcaccctg gttcgcttgg tcctcgaatc cggttcgtcc
     6781 ggtatttccc cgtacggact caccaactca tcaggcaggt cttctgactt acggatcgcc
     6841 ctactctccg cgccttcccg tcttgctcgc gcaaaacagt ggccctatcg gatttcgtcc
     6901 ccgtttacag cggcgggacc gctcccggat ttcacgggat tccctattaa accttgcggt
     6961 acctgaggag caacattctg gatgttcccg gcacctctat aacaatcgaa gacatttgtc
     7021 aataacttaa gatcgggcga tttgcctgac atcaatcccg tttcgtcgtt cccgcccaag
     7081 ccgggaaccc ctgaagaccg tgcatgccct gtggggacgt ccgccttggc gggcatgacg
     7141 ccctgaggct ctcgaaatcg ctcaaatcag gtctgcttct ccaggcgttt ctcctcccgg
     7201 gtccaaggac caacccgcct cggtgttgcc ccgagacgag atcgaggcgg tcccgagacg
     7261 ccttccgtca tcccgtggat ctcgcggtca agctgctgtg ccgttgcggt cgaaggatga
     7321 cccccacgcg ggggagccat tctcaaagag gcaagggaag ggccacgtac ccgcgccata
     7381 tccagggcaa aagacccacc acccccagga tcacgcagaa gatcgctttg ccttttcctc
     7441 gaatgcggcc cttggagccc cacacgatcc tggaggctcg aaagccgaag aggatggcaa
     7501 ccaaagatcc caggcccaac agccagccaa atccaaagat gatcccccac ttggccagcg
     7561 tattcacttc cgcttgcagc tcgtcttccg tcatactgga aatgtcgctc acgagtgtcg
     7621 gctcccgtct ctgcccgcta atccaaattt ccatcgagac cttcctgcag atccattccc
     7681 ctataccaga aaacggccgt ccgggaaacg agaaaccccc caccctcccg gccggcgttc
     7741 cggcaatctt gccgggaagc acgaaccgtg ggcttttccg atcctcccgg tcggattcca
     7801 agccactgcc ttgtgcgacg attcgatatt gacaattaaa tgaggagatt taggttgttg
     7861 tcgtgttgtt gagagtcact cctgaaagga tagcggttag gaggctgcca tgaacaccat
     7921 gaagaagata gggacgggaa tgctgatggt cttgatcctg ggcgggatgc tgagctcttc
     7981 ttttgctctc gacaagggag ggggctggga agatcaggca aagagttatg aacgggatcg
     8041 tggctttatt gtaggggatt gacgtttgaa cctgtttgag accccgaggt ccgggtatcc
     8101 cttccgggtc ttggtgtgtg ggataaagga gtggaacatg aacgcgattc gaacgattgc
     8161 cgcagctttg gtgatggccc ttgtactggg cagcgccggg accggtcttg ccgccctgga
     8221 tcgaggcgct acggtgtata ctcccggagc ctttgattcc aacattgcgc aacccaacga
     8281 cgactaaccg aggaccctct ccggcatttg cacctgtaac gggcctgcac gggtcgtacc
     8341 ctgcagatag gggactgaaa aagaaaggca cgagaaggaa acttcttggt gccttttttt
     8401 cttacagctc cggccctacc accgttcctt tggacctcaa ccccgccgcg gggggagtgc
     8461 ctgtctgcac aaagtgcttg atcgactagg gtttttgtgg tttaggaatc attctgtact
     8521 aacccgattc ccttttcccg gggagcaccg gatgaccccg tcggtgtcgg acatccctat
     8581 ctgccaatgg aggtatggcc catgagtccc agatggaaca tgagggttgt tgcgcttttg
     8641 atttgcctgg ttgtctttct gccgtgtttt tctgtccacg ggtcccagga cgagcagttt
     8701 cctcccgggg ctcagctggc ggccttcaac cttccgggac ccgattccgc ccaagtccag
     8761 gcgtacctgg gcctgaagac cagggacccc tttacgatct ccaagattgg ggcgaagctt
     8821 gtctacatcg aatttctggg ggccatgtgt cctcagtgcc acgtgaatgc ccccgtggtc
     8881 aacaagctct accgggtgat ccaggacgac ccggctctgg ccagggacgt gaaaatcatc
     8941 ggaattgccg tcggcaacaa tcgggcccaa atcgaggcct acaagaagaa tttcaaggta
     9001 gcctttccca tattccccga cgaggacctt tccatttccg cagccgtggg gggagcagac
     9061 acgccgacca tgatcctggt caccaacagc ggcaagatcc tctcgagtca tcgcggggtc
     9121 atcacggact tcgacggcta tttgaagcag ttgagggaat tccacaagaa acagtagacc
     9181 accatggccg agaacgagaa taaagggaac cgactgaagg aaagcaccat cttccgaggg
     9241 cttccccagg agaaactgga cgaggtcgcc agggcggttc agaacgtggt cgtgccgggc
     9301 aactccctca tttttcgtga aggagatccc ggggacagct tttacatcat cagttcggga
     9361 agggtgaggg tctttcgaag gctgggcggg gacatggaaa cggagctctc ggtcctggga
     9421 ccgggagaca gcttcggcga gatggccttg ctcaccgggg agccccggtc cgcagacatc
     9481 gagaccctgg aagaaaccca cctgatggtc ctcaccaagg accagttcga tcacatcttg
     9541 agggactgcc ccgatatttc ccggactttt gtgaaggaaa tgaggcggtg gctccttcga
     9601 gaccaggagc tcctggaagt ggaggcccgg gaagcctaca aggccacccg gctttcctgg
     9661 gtcgatttcc tggtggtgtt cgcggtgagc attgtcctgg ccttgatttt caactcctcc
     9721 aatcccaacg ggattccctt gtttccgaag tttcccgacc gggcctcttt cccttccgtc
     9781 agccctgtgg aggccatgga agccgttcag cggggcgatg ccctactggt ggatgccatg
     9841 ccttccaact tttttgccaa acgccacatc caaggggccg tcaatatgcc cctggccctg
     9901 ttcgacatcg tctacatgat gagcttcgcc gaagaggaca aggagaagaa gatcatcgtg
     9961 tacgggggaa ccgtgagcaa gctctatgac ctcgaagtcg ccaacaagct cctgcttcgg
    10021 ggatattcca acgtccgaat tctcgagggc ggattgtcgg cctgggaaca aaacggctat
    10081 ccggtggtgg agaaggcgaa gaaatgatgg gagctttcat caagcgtatc gttaccagtg
    10141 agtacctggc tttcgtcctc aggatctacg tgggctattt cttcatctac gccagcatga
    10201 gcaagattcc ttatcccgcc cagttcgcgg aagcaacggc caactaccgg attattccgt
    10261 actggcttct caattccggg gcggtgtttt tgccctggat tgagttcgtg gcgggcttgt
    10321 ttctaatcat cgggttcatg tcccgggctt ccgtggtcct gatcggttgc atgctggtca
    10381 tgttcaatgt catggttctg atcaacatgt attggggggc tcccatcagc tgcggctgtt
    10441 acgataccgt gggggaaccc atcggatgga agaagatcct cgaaaacgcc gtcatgctgg
    10501 tctttgccgt ccagatcttt ttttacgatc ggctgttcat tttccgccgg ggaggattct
    10561 cacgggctgc cagaaaacgg gtgccttctc ctgccggcgc acgataggtt gtctcatgct
    10621 aaacatttca agatcccaca gtgttctatg gctccttcct ctcttcctgg tcctggtttt
    10681 ggtcctctcc tgcggatccg acaaccagat ggtgggtacc tacagggctg aaaccaaaga
    10741 atccgcaaaa caggcggagg tcctcgtgga gctgaaggcg aacggggagg gtacctggaa
    10801 gatgggcgaa gaggaagtcc ccttttcctg gtacctcaaa cggggagagc ttcgagtgaa
    10861 caccaagggt gggggcgtga tcgtgggcac catcgacaag gacgtcatcc acatgaccct
    10921 gccgggaacg gggggcttgt ccttcaagaa ggtgcaatag ccgggctttc attcccgagt
    10981 ctccccgttc cgtactcgat ggtggtttcg gggttccctc caaagccctg ccggaggagc
    11041 cgttcgtcct gcgccgggat gttgccgccc ctgccgccgc ccttccccga aggcgtgggc
    11101 agcggacggc ggacccgcgc ccctccttcc gtaagagaac gatccaacgc gttccaaggc
    11161 gccgatgctt ccaaaggagt gatccggtgc ggctgagact gggccacaga atcctcgtca
    11221 tgtacgtcct tttcggggcg tgtttctccc tgttcacagg caccctggtc ttctctcggc
    11281 tcaaagaagg acgattcacc aaggtcggtt cccagttcca gcaccagctc aaccacatcg
    11341 atctcgccct gacgctttac ttcgacgagg ccgaggcgga cctgggcggg ctggcggggg
    11401 acgagttcgt aaggtccttg acgggagagg attcctcccc cggtgcccgg acggatcccc
    11461 gggtctttcc ctaccgcgag ccggacgtca aacggagggt cctgcacctg ttccgctcgt
    11521 tcaaggacgc ccacaagcac gtcacgtccc tctacctggc ctgggaaggg ggagggtacc
    11581 tggaatatcc ttcccgggag cggccgccgg gattcgatcc ccgggaggtc tcctggtacg
    11641 ggtcggccaa ggccgccgcc gggaaggtgg tgcgcacgga tccttacatt tcccatgcct
    11701 cttcggccat cctcatctcc atgaccgccg ccctcgtgga cgaaaacggc cggttcctcg
    11761 gcgtcgtggg gatggacgtg aattttgccg acctcaaggg ctacctcaag ggggtcaggg
    11821 cgggccagac gggacaattg gtcctggtcg accaacacgg cattctcctg gccgatccgg
    11881 acctggccgc cttttcccgg cccatttctt ccgtctacgc gggggacctc gcccccctgt
    11941 tcgaaagaca ggccgggttc gtgtccgtct cccgggccct gcaaagggac tacctcttct
    12001 tcacgacctc gaaaaagatg gggtggaagt tggggctcct ggtccctcag aacgagatcg
    12061 accgggaaat cgaaagcttc gtttacggca tcgtggccgc ggtccttctc tccattgccg
    12121 tcttgaccgt gctgagcctc ctggggctgc ggcatttcgt ccttctcccc atccgaaacc
    12181 tgcaccgggg caccgagctc atcgcccgca cgggagacct ggggcaccgg ttcgaggttc
    12241 gttccccgga cgaaatcggg gacctggccg cgtccttcaa cgccatgatc gagacgttga
    12301 gggaagccaa ggaggccctc aaggaatccc agggggaatt gaaaaggcac cgggaccacc
    12361 tcgaggaact ggtggaggcc cgcaccgtgg acctcaagaa gctctcggaa gcgttgaagc
    12421 agagccccgc ctcggtgatc atcacggacc agcagggaaa catcgaatac gtaaacgccc
    12481 ggttttgtga agtgaccggc tattccctcg aggaaaccct gggaaagaac ccccggatct
    12541 tgaagtccgg caagcacccg ccgtccctgt accgggacct ttgggaaacc atcctggcgg
    12601 gcaagacctg gagaggggag ctgatcaacc ggaagaagaa cggggaggaa ttctgggaaa
    12661 gcgcctccat ttcgcccatc atggacgacc ggggccggat cactcacttc gtggccgtga
    12721 aggaggacat taccgagcag aaggcggtgg aagcgagact gcaactcacc cagatggccg
    12781 tggatcatat tttcgagcaa accctgctgg tagaccggga cgggcggctt tgcatggtca
    12841 acgaagcggc ctcccgggtc ctcgggtaca ctcgagaaga actgctggac atgtccgtgt
    12901 gggacctctt cgaaggtttt cccccgggaa gctggccgga gaaatggcag gaggtgaagg
    12961 cggccggatc cctggtattc gagtcttacc atcgcacccg ggacggtcac acttaccctg
    13021 ccgaggtcac cgccagtttc ttccgctacg agggacagga atacgtccac gccgtttccc
    13081 gggacgtgac cgaacgcaag cgggcgcagg aggaactggc cgaggccgag gagcgaagtc
    13141 gcctgctcct ggaatccgca ggggaaggaa tcgtgggggt ggatcccagg ggcaaggtca
    13201 ccttcgtgaa cccggcggcg accctcatgc tcgggtatct gcccgaggac ctcatcggtc
    13261 ggggcctgca tcaactcatc catcattcct atgccgacgg ggccccctat cctgccgaaa
    13321 actgccccat gttcaagtcc tattcctacg ggattgtcaa cgaagtggag gacgaagtcc
    13381 tgtggagaaa ggacggcacc tgcttcaacg tggaatattc gagcaccccc gtctggaaaa
    13441 acgacaccct catgggagcg gtggtgacct ttcgggacgt caccgaacgg aaacgaatgg
    13501 aggcggcgct ccgggaaagg gaggaatatt tccgggccct gttcgaccat gcgggggtag
    13561 ggatcgtcag caccgacaac gaggggaggt ttacccgggt caacgagcag tttctcgagt
    13621 tcatcggcta tacctgggag gaactcaaga cccttcccct ggtggccctc tgccatcccg
    13681 actaccggga aaaggccaag gaagccatgg aacgactgag aaacggccag atcgacctca
    13741 ttcgcaccga gatgctctat gtaagaaaag acggggttcc ccgctgggcc gacgtccgat
    13801 cgggccccat tcgagatgcc gagggcaacc tcatcgcatc ggtcaccacc atcaccgacg
    13861 tcacggaacg gaagcgctcc gaagtggaac aggcccggcg cctgcgcacg gagaaggcca
    13921 tggccgccgt atcccgggcg ctcctggggt ccgacaccga gagcgaaacg ctccaggacg
    13981 ccctcaaaca aatcgtggcc gccgcccagg tggaccgggt ctatgtctac cagaacgtgg
    14041 aggaccccgc ggagggtctg tccatgtggc tgattttcga ggcttgcgcg ccgggcgtcg
    14101 agagcatctc ccagaaccgg gagatggtcc agcgccccta cgcccggggc ctggcccggt
    14161 ggcaggaaac cctgggtcag ggaaagccca tcatgggtcc cgtgaacagc tttcccaacg
    14221 aggaacagga attcctccgg ggtcacgaaa gcctttccac cctccttctg cccatccagg
    14281 tgaagaagga atggtttgga ttcatcggcc tggacgacaa tcacctgcgt cggaactgga
    14341 cctcgtcgga cgtgactctc ctgggaagca ccgcggagat catcggcgcc ttcctggggc
    14401 gccagagggc cgaagaagag ctccgaatcg ccaaggacaa ggcggaagag gccaccaagg
    14461 ccaagagcgt attcctggcc aacatgagcc acgaggtccg cacccccatg aatgcgatcc
    14521 tggggatgac ctacctggcc ctccagaccc ggctcacccc gaagcagaag gactacctgg
    14581 ccaagatcca gcgctcgacc aagtccctcc tgggaatcat caacgacatc ctggatttct
    14641 ccaagatcga ggcgggcaag ctccagatgg aggatacgga tttcagcctc gaggacgtcc
    14701 tcgagaacgt ctccgccgtc gtggggatca aggcccacga aaaggaactc gagttcctca
    14761 tggacacggc cgcgaacgtg cccacggccc tccacggcga tcccctccgg ctggggcagg
    14821 tgctcatcaa cctgtgcaac aatgcggtaa agtttacgga caggggcgag atcgtcgtct
    14881 cggcccgggt ggcggagcgg ggcgaggatt gggtcgtgct gcagttctcc gttcgcgata
    14941 cggggatcgg gctgagcgaa gtcgagaagg aacggctctt cctggccttt tcccaggccg
    15001 atacctccat caccaggaag tacgggggaa caggcctggg tttgaccatc agcaggcgat
    15061 tggtccaaat gatgggagga aagatccggg tcgagagcgt tcccggccag ggcagcgaat
    15121 tcatctttac ggcccggttc ggcctgtcgg gcaagggggc tgcccggacc ctggaaccct
    15181 tgcccgacct gagggggatc cgggtgctgg tggtggacga caatccttcg gcacgggaga
    15241 tctttcagaa cctgctcgaa agcatgagtt tcgaggtttc cctggcggct tccgccgaag
    15301 aggccctatg ggagatcgaa aaggaggcgg gcggaaggcc ctacgaactg gtggtgatgg
    15361 actggaaaat gccgggcatg gacgggatca aagcttccga ggcaattcgg cgccacccgg
    15421 gtcttccccg gcagccccgg atccttctgg tcacggccta tgggcgcgag gaaatcatga
    15481 cccgggccga aaaagccggc ctggacggat tcctcgtcaa gcccgtcacc caatccatcc
    15541 tgttcgatgc catcatggat gccttcggca ggagagatcg ccgggaagac ccggggggca
    15601 gatccgcccg ggaggctgcc gaatccttcc atcgaatccg gggggcgaag gtgctggttg
    15661 ccgaggacaa cgagatcaat cagcaggtcg ccgtggaggt cctgcagagg gccgggcttc
    15721 gagttcgggt ggtggacacg gggtcccggg cggtggaaaa ggtcctcgag gaacccttcg
    15781 acgcggttct catggacatc cagatgccgg agatgggggg atttgaagcc gctcgaatcc
    15841 ttcggaaaga cccccggttc cgagacctgc ccatcattgc catcaccgcc cacgcaatgg
    15901 cgggagatcg agaaaagagc ctcctggccg gaatgaccga tcacgtgacc aagcccatcg
    15961 accccgatga gttgctctgc gttctggccc ggtggatccg gccccgggaa ggggaattcc
    16021 cggaatgcga accggctcgg gaatcggccg aagaaggtcc tacggatttc ctgcccgaaa
    16081 atcttcccgg gatctccctc gaggccgggc tgaagagagt cgggggggat cgggccctgt
    16141 acgggaagct ccttgcccgg ttccgacgca gccaggccga caccccggac aaagtccgca
    16201 ccgccctcga acaggaagag acgcggacgg cattgggact tctgcatacc ctgaaagggg
    16261 ttgccgggac cctgggagcc gagggtcttg ccgtggccgc ctcgaggctg gaagaagccg
    16321 tgaacgcggg acatcccggg gacatcgatc cttgtcttcg aaccctggcc gagagattcc
    16381 aggaagtttt cgaaggcctc gaggaatggg aaggagcgga gtcccgaacg caacctccca
    16441 tccgcccggc gacggaggat tccatggaca gagaccgggt ccggaggctc gtggaggaaa
    16501 cgggtcgact cttggaaacg gaccttcccg aggccatcct tcgcttcgag tccttgaagg
    16561 accacctccc ccggtcttct ttcgaggaag agatcaaggg tctcgaaagt tccctcgaag
    16621 ggttcgatac ggacgccgcc ctgggctttc ttgccgtcat cgccgccggg ttggaccggc
    16681 cccgggaggg agaataagaa atggcggagg gttcccccaa gcccaaagtg ctggtcgtgg
    16741 acgacacccc ggaaaacatc caggtgctca tgggcaccct caggggagac tatgccgtgg
    16801 tggcggccac caacggcgaa aaggcactgc ggcttgccgc cgcgcaaccc cccccggatg
    16861 tcattctcct ggacatcatg atgccgggca tggacggctt cgaagtctgc cgaaggctca
    16921 aagccgtgcc ggaaacccgg gacattcccg ttctttttct ttccgccctc gacgaggttt
    16981 cccacaaaat cagagggttt tccatcgggg ccgtggacta catttccaag cctttccagc
    17041 cggaagaagt ccatgtgcgg gtcaacaccc acctgaccat caaccgcttg aggcgaaagc
    17101 tggaagagaa acgcagggag ctcgaggtct acaatgagag tctcgaggag cgtgtgcggg
    17161 ccaggaccgc ggaactggcg gccctcaacg aggtttatga gcgctttgtt ccccgggagt
    17221 tcatcgacct cctgaaaaaa agcagcatcc tggaggtccg actgggagac caggtgaaac
    17281 agcgcattac ggtgatgttt gccgacatcc acggctggac cggcctgctg gaacggatga
    17341 gccccccgga tgcctttcgt ttcttcaacg actatctgag cagggtctca ccggagatca
    17401 aggcctgccg aggttttatc gaccagtact acggcgacgg catcatggcg cttttccccg
    17461 gtcccccgga cgatgccgtg cgggccgccg tatccatgca gacggcggtt ttcgagtaca
    17521 accgggcacg agaacaagac ggtttggagc ccatccaggt ggggatcggc cttcattttg
    17581 ccgatctcat gctgggaatc atcgggaacc gggaccgcat gcagggggcc gtggtcgggg
    17641 acgacgtgaa cctggcttcc cgcatcgagg ggatgacccg gatgcacggg gctcgaatcg
    17701 ccctcagtga acagaccctc ctgcagatcc aggacccgac ccggttcaag caccgctgga
    17761 tcgataaggt ccgggtcaag ggaaggcggg agcccgtcac ggtctacgag gtgttcgagg
    17821 gagacccgcc ccccgaggcc cggctcaagg aatccacccg gacccagttc gaagcgggat
    17881 tgcggctcta ctacgggaga aaattttccc cggcgagcgt ccagttcaat ggagtactcg
    17941 aaaagaatcc cgaggacctt gcctcgagga tctatctcga acgctgtgcc cgctacatgg
    18001 tgaacgctcc ccccgacgac tggacggggg tcgagtccct cgaagaaaag tgaggattcg
    18061 gccgatcccc tctgatgagc ggaccgcgaa cgatccgctt gtctcggcgg tcgagccgct
    18121 gcccttcacg cccctt
//
LOCUS       DAOOVC010000071        18074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2120_length_18074_cov_6.556357, whole genome shotgun sequence.
ACCESSION   DAOOVC010000071 DAOOVC010000000
VERSION     DAOOVC010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18074
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2120_length_18074_cov_6.556357"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..2603)
                     /gene="hdrA2"
                     /locus_tag="PLM79_10320"
     CDS             complement(171..2603)
                     /gene="hdrA2"
                     /locus_tag="PLM79_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(1992..1994),aa:Sec)
                     /transl_table=11
                     /product="CoB-CoM heterodisulfide reductase HdrA2"
                     /protein_id="HOV86747.1"
                     /translation="MEKLANKSGEPRIGVYVCHCGLNIARTVDCGKVARRSSNLEGVV
                     VSQDIPYACSEPGQVKIREDIAEHGLDRVVVASCSPRLHEPTFRQMMQAAGLNPYMME
                     MANLREHCSWVHMHEPDAATDKAFDLVKMAVSRIRGLQPLTPELLPLTKRTLVIGGGV
                     AGIQTALDLADNGYEVVLVEKRPSVGGMMARLDKTFPTMDCSIUILGPKMTDVGRHPL
                     IQLLTMSEVTEVKGYVGNFEVKILKKARYVDEKECTACGDCAAVCPVVRPDGFNLGLS
                     SRKAIYSPFPQAVPSAYVLNANECLGHNPAVCAKCIEACKKGCIHFNMSDEEISVKIG
                     TIVVATGIEPYDPTEMDEYGYTRFQNVLTSLEFERLVNAGGPTKGELIRPSDRRHPRS
                     IGFVQCVGSRSARKGRSYCSNVCCMNTIKSTLVLKEHHPDLEVKVFYIDIRAFGKGFE
                     DLYKRSRRLGVQYIRGLPGTVEETGDGSLRVAVENTATGAIEFHDLDMLVLAVGVNPS
                     ESTRKLQEMLGLQLTSDGFFLEAHPKLQPVDAATRGIFYAGCAEGPKDIKDSVTQASA
                     AAARAIRLMHKGSILTEPITSEVIAEQCKACGRCAEVCPYNAITVDTKKKTPAVVNTA
                     SCAGCGTCAAECPFGAITMNHFTDAQILDQIDAMLAENPGEKILAFACNWCSYAGADY
                     AGVSRLQYPSNVRLIRTMCSGRVDEEFIWHGLARGAPVVLVSGCHIGDCHYINANHWT
                     EKRVAKVHRKMAGLGIRPERLQLEWVSAAEGIRFAEVMKRLDALRQTVTEEEIAETIG
                     KLTQKRVKEAVS"
     gene            complement(2665..4248)
                     /locus_tag="PLM79_10325"
     CDS             complement(2665..4248)
                     /locus_tag="PLM79_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV86748.1"
                     /translation="MDTLKIILFMCNWGPHAAFQTLLDDGSEIPPQVRMVRIPCSGRI
                     SKSLLFKAFEMGADGVALVGCEPGSCRYGNGPNLAQFHVNDTRDILDLLGLGKERLRW
                     GTFLPEQSSSLLSFLQDFTGAVAQMGRSPVVPQSKIAAVPVPREQTMQQVVAHDVYAC
                     QECGKCTSACPLALAGKPFSPRAIANSVISGTSNGESVAKDVWACLTCGLCYDRCPSA
                     VNFPNFVRDLRQAHHDCGQCGHEPHDGFFHALMRTMTSPDLQVRHWDWVPKDLGVDPE
                     GKLLFFGGCAPYFDTFFRHHMPTRTQDILLDSIRLLNFFDITPRLLSRERCCGHDLLW
                     SGDKANFLELAKLNGQAIRESGVEEIVTSCPECYRTLSEDYPRHGIDLGCKVTHLYDL
                     LEREIDKGAVGFEALNRRITYQDPCRLSRMENPSDAPRKLIHRLQSRGFVEMKDRGAS
                     SLCCGNCAWTTCDSYNKAMQLKRLEQARDTGSDLLVTACPKCQIHLTCAMEGPFLEDG
                     LRIEMADLVSVLAKTIRWE"
     gene            complement(4248..7604)
                     /locus_tag="PLM79_10330"
     CDS             complement(4248..7604)
                     /locus_tag="PLM79_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86749.1"
                     /translation="MKAPKDRLHRVLVIGATPAGLAATNKLGEMGIPVVLVDSDPDLH
                     GKLAREEWRLASGLTLNYALRPGLLRILRNPRIRCVLPGEVKTLKHTPQGFSARIVHN
                     PAFVDPERCTLCGLCARICPVTSIGGLKPIQYNGRQSLPGRPVIDKRRQPLCQANCPL
                     GVNVQGYMALAKEGRYGEALEVIRRDNVLPGICGRVCSHPCESACRRGELDEPLAIRD
                     IKRFLADYAPADAEDAAAPALPTRPQKVAVVGSGPAGLAAAADLARFGYPVTVFEKEE
                     SLGGLLRYGIGLHRLPREILDREIRYLQGLGIRFLTNHPVDPRNELEKLREEFSAILL
                     TTGVWTDRKLGVPGEDLEGVSGCLEFLSRFHRGQAEDLGEHVAVIGDGNAAFDLARGL
                     ARTGRTVTLLSWFPEEMIPADEEEIRGAREEGIRLVDRTQTIGFTGSNGRLQSLRCIR
                     TQPGEPDAKGIPWPVPVPGALPFDLSFDSAIVAIGQRGDRFLLGTDQPVGMTDAGYIA
                     VDDLFRTNVPGVFAGGDVIRGPSSVVEAMASGRAAARAIHGQLSGEKPAVSSPWRPRE
                     KEFPSIPPDLPSLARPKMPERRVSARMESHVEVALGLDEKQVLSETSRCLQCGVCSEC
                     LQCAEICSAIGAIRHEQGPVEEVEQAGVVIVADPDASPAVKGEDVLRAYSTKSAKDDS
                     HAMMLRGFAAAAEALTLLGSGAHRLKGQGLSFSPPDPLLSPDIRIGVFVCRCNDSLGW
                     PPEFDEYVRGLVRRPQVEHAEILASACTPEGSAGIVRAIRERGLTRAVLASCVCCPLD
                     FVCSACTDQRTRLKSSLFHGTGIGRAMVETCNVRGEALRFLQGDRELAFERFTGLVDR
                     SVGRAGRLKALPAPARPYNFTTAVIGESEAAVKSALTLAEMGMEVFYFGSPEHAPSKT
                     LRQSGIHCFPGAVITGIRGTLGDFGVIAQVEGVEQTYRVGAIILGEQARKRVPFRSGA
                     DLPPRIVPAAMQKKGVAGVPFFYPGSTPIPGVFLANPKEIRVSERIKGAAAAILAASV
                     MPRRPRQSKGYTLVVDEDLCRGCGRCVQVCPYQAVGFRKKATGSWCAVVDEALCKGCG
                     NCVSVCPSNAADSPYRNRRYLEEMVEEILL"
     gene            complement(7601..8224)
                     /locus_tag="PLM79_10335"
     CDS             complement(7601..8224)
                     /locus_tag="PLM79_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV86750.1"
                     /translation="MNQGTRTQQGNPVSTRKEILITGFGGQGIVLAGLIVGRAASLGD
                     RKESTLTQSYGPEARGGACSAQVIVSDTTIHYPYVRRPDYLVCMSQGGYEKYSPLLPE
                     GGTLLLDSDLVQVEQAKFANLFAIPSTRMAEELGRKMMANIVMLGFFTAVTRALSVEA
                     ARNAVADSVPKGTEAMNIQAFNKGHDYGEAILKGRQKKAEGKKGVRA"
     gene            complement(8239..9102)
                     /locus_tag="PLM79_10340"
     CDS             complement(8239..9102)
                     /locus_tag="PLM79_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOV86751.1"
                     /translation="MNRPRPKSDHPLDELLRTDRVPHIWCPGCGIGTAFSSCLVAVKN
                     SGIDLVKTVMVSGIGCSGRAAGYIKLDSYHTTHGRAIPFATGLKLANPELNVIVFSGD
                     GDLFAIGGNHFIHAARRNMDLTVICVNNFNYGMTGGQAAATTPTMARTTTTPFGNPEP
                     PFNLPLLAFASGATYVARWTILHTRDLTKSMEEAVTRRGFSFIEVLAPCPTGYGRRNK
                     EKPLDTLRMYQERTIIRNDANPAEAVMDYERGITLGKFVDVESPTFLERYERSSRPAP
                     PTAASDRGGCA"
     gene            complement(9103..10254)
                     /locus_tag="PLM79_10345"
     CDS             complement(9103..10254)
                     /locus_tag="PLM79_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOV86752.1"
                     /translation="MKPAVLTGEHFISGDAACAEGALAAGCRFFAGYPITPATEIAEH
                     MAHRLPDVGGVFIQMEDEIAAMAAIVGGSCAGVKSMTATSGPGFSLMMENLGLAVCTE
                     TPCVIVDVQRAGPSTGLPTLGAQGDMMQARWGSHGHYEIIALAPASPQELFYQTITAF
                     NLSERFRTPVLIMTDEAVGHLNERVVIPESGAIRTVNRTLPKGRKDRFKPFRAGPDGV
                     APMAVAGTGYGVHITGLTHNEQGYPVMTAEAQAAMMERLTTKIRRNLDQIIRTESYRL
                     EDAEIALVSYGVSARSSFSAVDEARNMGIRAGLLRLVTVWPFPEEQIRRLARQVRALV
                     TVEINLGQIHLEVQRCAGDRAPAFLVGHSGGAVIPPEQILETLKQIQGV"
     gene            complement(10309..10596)
                     /locus_tag="PLM79_10350"
     CDS             complement(10309..10596)
                     /locus_tag="PLM79_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86753.1"
                     /translation="MKFWRTPLDTNLIQVTRGIVHIIEERCKGCGYCIEYCPRDVLEI
                     SAKFNNKGYHPPRVKKPDLCVNCRYCEIICPDFAIFSVEAVPSDPAPAPAQ"
     gene            complement(11181..11786)
                     /locus_tag="PLM79_10355"
     CDS             complement(11181..11786)
                     /locus_tag="PLM79_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV86754.1"
                     /translation="MNLVAIVGSPRKGKATDRLVDRALEGVQSGTEGLRVKKINLVDY
                     DIGYCRNCLSCRDAKTRGPMAPCVLRDDMDGIREDLLASDLLVVGTPVHMGSVTALMM
                     TFLERICWTFAKPEGRVFTLRGCPVPRSKKRRKAVIIVVSGIVPPIYRRFCDEATALI
                     RETLKDSLNAKTVGDMYAGAVEHRGVEHYFEKAFALGRKLA"
     gene            complement(12483..14951)
                     /locus_tag="PLM79_10360"
     CDS             complement(12483..14951)
                     /locus_tag="PLM79_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86755.1"
                     /translation="MSKAFAELSAKDLRATVDLDSLPFENTSSLEALEERVVGQERAI
                     DAIKFGMGMKESGYNIFIAGPPKAGLTYIGKTYLEEQAKEEPTPPDWCYVYNFKETDK
                     PKSLKLTAGRGKVLRKDMQEFINTLQAKIPEVFDSDDYRTKESEVHQSFERLRREIID
                     ELSAQAKAEGFILQFSQVGMVIIPANKEGEPMTQEDLRHLEEEDRKVLREKSDQLHEK
                     MKEAIKRIREAEEEFKQKHSKLDSEIALFVVGQLMDSYEEKYQDEPQVLEYFKMVQED
                     ILENIEEFKKKPEAQQQQQPGAPFPMPPREASLRKYEVNVLIDNSETKGAPVVIESNP
                     AYPNIFGSIERQAWFGALFTDHTMIKPGALHKANGGYLIMKALDLLRWYITYEALKRA
                     LRDQEIKIEDLGELYGLFSTRTIRPEPIPLQCKIVLAGDPYIYQLLYTYDERFQKYFK
                     VKAHLDDQMDRKDEKILECARMMSHYCKEHQLRHLDRSGVSRVIEYSMELTQDQKKLS
                     LEMGSISDLLKEANYFAGLDQAEFIQRKHVQEAIDKRTYRSNLIEDRIKDLTERDIFW
                     VETDGYKVGQVNGLSVLMTGDHVFGKPNRITANVSVGREGMISIDRESKMSGNIHTKG
                     LLTLSSFLKERFAHNKPISLTATLSFEQSYGMVEGDSASSTELYVLLSALSGIPIFQG
                     IAVTGSVSQKGEVQPIGGATYKIEGFFDICKFKGLTGKQGVMIPSKNVDNLMLKQEVV
                     DAVEAGQFHIWPVTTIEEGIEILTGVEAGKLQEDGTYPEGTVFRKVDDRLRKIAEIVK
                     EYGKEGEDGKKKEEEPAGCPHCNV"
     gene            complement(15009..15239)
                     /locus_tag="PLM79_10365"
     CDS             complement(15009..15239)
                     /locus_tag="PLM79_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOV86756.1"
                     /translation="MKELVEYLVKSLADHPEEVFLNEQEAEDTVLLELKISPEDLGKI
                     IGKNGNTINAIRTVLQAAASSHKKRVKLEVVS"
     gene            complement(15297..15593)
                     /locus_tag="PLM79_10370"
     CDS             complement(15297..15593)
                     /locus_tag="PLM79_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOV86757.1"
                     /translation="MAIVEVSVVPLGVPDTSLSHYVAGALKVLKHSGLSYELTAMGTI
                     ISGDLDEVWKVVRQMHESCFARGVPRVLTQVKIDDRRDRPGSPELKVRSVMDKL"
     gene            complement(15596..16432)
                     /locus_tag="PLM79_10375"
     CDS             complement(15596..16432)
                     /locus_tag="PLM79_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HOV86758.1"
                     /translation="MPPEPEEKLQSLLRSVHVNIPWRELPKYVDTILHLRLNVEIGIE
                     ATVLDELSRPEIVAVVSQLRREGCRITLHGPFWDLSPGSVDPLIRRASRRRLQQFFDV
                     VDSFRPIQVVCHTGFDPRHHGGHRSLWLDESLTVWEILVRRGEDLQVPLLLENVWEYG
                     PDLHKELFGRIPSPYFGFCLDVGHQHSFSRTPLTAWLDELQDRLREVHLHDNDGSGDA
                     HLPIGDGTIDFVGLFRFLGSRGMKPVLTLEPHREEHLAGTLRGLNRMLSAAGWNSFRE
                     GS"
     gene            16583..16915
                     /locus_tag="PLM79_10380"
     CDS             16583..16915
                     /locus_tag="PLM79_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86759.1"
                     /translation="MKQIVYDQLRELDYFKIRDYLDENVEKGTLEGIYQVDLPPGLHS
                     RTQAEHARCQGFYFAVNLDRNQVAFELLIRSRQVLRCSCIGYATPEQRDYIFAFADQV
                     LEDLGIRI"
     gene            complement(16931..17986)
                     /gene="aroC"
                     /locus_tag="PLM79_10385"
     CDS             complement(16931..17986)
                     /gene="aroC"
                     /locus_tag="PLM79_10385"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOV86760.1"
                     /translation="MAGNSFGRLFRITTWGESHGPGLGVIIDGCPPGIPLTVEDIQKD
                     LERRRPGKRLTTSRQETDRVEVLSGVFEGVTMGTPISLTIRNRDVRSRDYEPLAMVYR
                     PGHADRTYDQKYGVRDWRGGGRSSGRETAARVAAGAVARKFLRQYDIRVQAYTISLGG
                     IRCRRFDADAVDQNLLYCPDMEAAKAMEERLAEVRAAGDSVGGIVEVRAQGCPPGLGE
                     PVFDKLDARLAAALMSIGAVKGVEVGEGFAAADMLGSEHNDPLTVEGYESNHAGGVLG
                     GISTGADLVLRAAVKPIPSISKVQQTITAEGKPTKIRIRGRHDVSAIPRIVPVCEAMV
                     MLVLADFLLHPFPEKVG"
BASE COUNT         3277 a         5478 c         5709 g         3610 t
ORIGIN      
        1 cctaatgaat tcaatcggtt agggtcgaag aaacgcactc ttcaggactt tttacgtaac
       61 catcaaagtt gagtccccgt tcggcctcat tctccggcgg cagggcccag aaaggatggg
      121 cacccccgaa ccacacggga ttggggggga agcatcctcc cgggacccgg tcaggacact
      181 gcctccttga cccgtttttg ggtcagcttt ccgatggttt cggcgatttc ttcctcggtc
      241 acggtttgtc gcagagcatc aagccgtttc atgacctccg caaaccgaat gccttcggcg
      301 gcgctcaccc actcgagctg caagcgttcg gggcggatgc ccagtccggc catcttgcgg
      361 tgcaccttgg ccacccgttt ttccgtccag tggttggcgt tgatgtagtg gcaatccccg
      421 atgtgacagc cgctcaccag gaccaccggg gctccccggg caaggccgtg ccagatgaat
      481 tcctcgtcca cccgtcccga gcacatggtc cggatgaggc ggacattcga gggatactgg
      541 agcctcgaga ccccggcata atcggccccg gcgtaagaac accagttgca tgcaaaggcg
      601 aggatctttt ccccggggtt ttccgccagc atggcatcga tctggtccag gatctgggca
      661 tcggtgaagt ggttcatggt gatggccccg aagggacatt cggcggcaca ggtgccgcaa
      721 ccggcacagg aggccgtgtt gaccacggcg ggggtcttct tctttgtgtc cacggtgatg
      781 gcgttgtagg gacaaacctc ggcgcaccga ccgcaggcct tgcactgttc ggcgatgacc
      841 tcggaggtga tgggttcggt gagaatgctg cctttgtgca tgaggcgaat ggcccgggcg
      901 gcggcggccg acgcctgggt cacgctgtcc ttgatgtcct tgggtccttc ggcgcatccc
      961 gcgtagaaga tcccccgggt ggcggcgtcc acgggttgca gcttgggatg ggcctcgagg
     1021 aaaaagccgt ccgaggtgag ttgaagcccc agcatttcct gcagtttccg ggtgctctcg
     1081 gacgggttga cgcccacggc cagcaccagc atgtccaggt cgtggaactc gatggccccc
     1141 gttgcggtgt tttccaccgc cacccgaagg ctcccgtccc ccgtctcctc caccgttccc
     1201 gggagccctc ggatgtactg cactcccagc cgacggctgc gcttgtagag gtcttcgaac
     1261 cccttaccga acgctcgaat gtcgatgtag aagaccttca cctcgaggtc cgggtggtgt
     1321 tccttgagca ccaaggtgct cttgatggtg ttcatgcaac aaacgttcga acaataggac
     1381 cgccccttgc gggccgaccg ggaccccacg cactggacga acccgatgga tcgcggatgc
     1441 cggcggtccg aaggccggat gagttccccc ttggtggggc ctcccgcgtt gaccagccgc
     1501 tcgaactcga ggctggtgag aacgttttgg aaccgggtgt agccgtattc gtccatttcc
     1561 gtgggatcgt agggctcgat tcccgtggcc acgacgatgg tcccgatctt caccgagatt
     1621 tcctcgtcgg acatattgaa atggatgcac cccttcttgc aggcttcgat gcacttggcg
     1681 cacacggcgg gattgtgccc caggcattcg ttggcattga gcacgtaggc cgaagggacc
     1741 gcctggggga agggggaata gatggccttg cgggaggata gccccagatt gaatccgtcg
     1801 gggcgaacca cgggacacac cgccgcgcag tctccgcagg cggtacattc cttctcgtcc
     1861 acgtaccggg ccttcttcag gatcttcacc tcgaagttcc ccacgtaccc tttcacttcc
     1921 gtcacttcgc tcatggtgag aagctggatc aggggatgtc gaccgacatc cgtcatcttg
     1981 ggcccgagaa ttcagatgga gcagtccatg gtgggaaagg tcttgtccag gcgggccatc
     2041 atgccgccga cggagggcct cttttccacc aggaccacct cgtatccgtt gtcggccaag
     2101 tccaacgccg tctgaatccc ggccactcct cccccgatga ccagggtgcg cttggtgagg
     2161 ggcagcagtt cgggcgtcag gggctgaagc ccccgaatgc gggacacggc cattttcacc
     2221 aggtcgaagg ccttgtccgt ggcggcgtcg ggctcatgca tgtgaaccca gctgcagtgt
     2281 tcccgcaggt tggccatttc catcatgtag gggttcaatc ccgccgcctg catcatttgc
     2341 cggaaggtgg gctcgtggag cctcggggaa caggaggcca ccaccactcg atccagaccg
     2401 tgctcggcaa tgtcttcccg gatttttacc tggccgggtt ccgaacaggc gtaggggatg
     2461 tcctgggaca ccaccacccc ctcgagattc gaggatctcc gggccacctt gccgcagtcg
     2521 acggttcgag cgatgttgag tccgcagtgg cagacgtaga cgccgatgcg gggttcgccg
     2581 gacttgtttg cgagtttttc catggtcctt ttctctggtc caatagggtt acgcggttgc
     2641 gggacgtctt gccgtaagcc cccgctactc ccaccgaatg gtctttgcca gcacggaaac
     2701 gaggtccgcc atctcgatcc tgagcccatc ctcgaggaag gggccttcca tggcgcaggt
     2761 aagatggatc tggcacttgg gacaggcggt gacgagcaga tcgctgcccg tgtcccgggc
     2821 ctgctcgagc cgtttcagct gcatggcctt gttgtagctg tcgcaggtgg tccatgcgca
     2881 attgccgcag cacagggagg aagccccgcg gtccttcatt tccacgaaac cccgggattg
     2941 gaggcggtgg atgagcttcc ggggcgcgtc cgagggattc tccatgcggc tgaggcggca
     3001 ggggtcctgg taggtgatcc ggcggttgag ggcctcgaag ccgactgcgc ccttgtcgat
     3061 ttcccgctca agcaggtcgt agagatgagt caccttgcag cccaggtcga tgccgtgccg
     3121 gggatagtcc tcggacaggg tgcggtagca ctcggggcag ctggtgacga tttcctcgac
     3181 cccggattcc cgaatggcct gcccattgag cttggccagt tccagaaagt tggccttgtc
     3241 cccggaccat aacaggtcgt gtccgcaaca ccgttcccgg gaaaggagtc ggggcgtgat
     3301 gtcgaaaaag ttcaacagcc ggatgctgtc caggaggatg tcctgggtgc gggtgggcat
     3361 atgatgccga aaaaaggtgt caaaatacgg ggcgcagccc ccgaagaaaa ggagcttgcc
     3421 ctcggggtcc actcccaggt ccttgggcac ccagtcccag tggcggacct ggagatccgg
     3481 ggaagtcatg gtcctcatga gggcgtgaaa gaacccgtca tggggttcat gaccgcactg
     3541 gccgcaatcg tggtgggcct gcctcaaatc ccggacaaag ttgggaaaat tcacggccga
     3601 agggcaacgg tcgtagcaca ggccgcaggt caggcaggcc cacacgtcct tggccacgga
     3661 ttccccgttc gaggtcccgg agatgacgga gttggcaatg gcccggggag aaaacggctt
     3721 gcccgccagg gccaggggac aggcggaggt gcacttgccg cattcctggc aggcataaac
     3781 atcgtgggcc accacttgtt gcatggtctg ttcccggggt acggggacgg cggcgatctt
     3841 ggactggggc accacggggc tgcggcccat ctgcgccacg gcaccggtga agtcctggag
     3901 gaaggaaagc agagaggagg attgctcggg caggaaggtt ccccaccgga gccgttcttt
     3961 gcccagtccc aggagatcga gaatgtcccg ggtgtcgttc acgtggaatt gggccaggtt
     4021 gggaccgttt ccgtagcggc aggagccggg ctcgcacccg accagggcca ctccgtcggc
     4081 ccccatctcg aaggccttga aaagaaggga cttgctgatc cttcccgagc aggggattcg
     4141 gaccattctc acctgggggg gaatctcgga accgtcgtcc aggagggttt ggaacgctgc
     4201 gtggggcccc cagttgcaca tgaacaggat gatcttcagg gtatccatta caggagaatc
     4261 tcctccacca tttcttccag gtaacgtcgg tttcggtagg gactgtcggc ggcgttggag
     4321 gggcaaacgg acacgcaatt gccgcacccc ttacacaggg cttcatccac cacggcgcac
     4381 cagctgcccg tggctttctt ccggaacccc acggcctggt aggggcagac ctggacgcac
     4441 cggccgcagc cgcggcagag atcttcatcc accaccaggg tgtatccctt gctctgccgg
     4501 ggtcgccggg gcatgaccga tgcggcgaga atggccgccg ccgccccctt gatgcgctcc
     4561 gagacgcgga tctccttggg attggccagg aagaccccgg ggatgggggt ggacccggga
     4621 tagaagaagg gcacgcctgc tacgcctttc ttttgcatgg ccgccggaac gattcgggga
     4681 gggaggtccg cccccgacct gaagggaact cgtttgcgag cctgttctcc caggatgatc
     4741 gctcccaccc ggtacgtctg ctccacgccc tccacttggg cgatgacgcc gaaatccccc
     4801 agggtccccc gaattcccgt aatcacggcg ccgggaaaac agtggatccc cgattgccgg
     4861 agcgtcttcg atggggcatg ctccggggac ccgaaataga aaacttccat ccccatctcg
     4921 gccagggtga gggcgctctt gacggcggct tccgattctc cgatgacggc ggtggtgaag
     4981 ttgtacggac gcgccggggc gggcagggcc ttgagccgtc cggctcgacc cacggagcga
     5041 tcgacgagtc cggtgaagcg ctcgaaggcc agttcccggt ccccctgcag gaagcggagg
     5101 gcctcccccc gcacgttgca ggtttccacc atggcccgcc cgattcccgt tccgtgaaat
     5161 aaagaggatt tcaaccgggt cctctggtcc gtgcaggcac tgcagacgaa gtccaggggg
     5221 cagcagacgc aggaggccag taccgcccgg gtgagccccc gttcccggat ggccctcacg
     5281 atgcctgcgg atccttcggg agtacaggcg gaagccagga tctctgcgtg ttccacctgg
     5341 ggacggcgga ccagcccgcg gacgtattcg tcgaactccg gaggccaccc cagggagtcg
     5401 ttgcatcggc acacgaaaac gccgatgcgg atgtccgggg acaaaagggg gtccggtgga
     5461 gagaaggaaa gtccctggcc cttgagacga tgggcgccgc tgcccagcag ggtcagggcc
     5521 tccgccgccg cggcgaagcc tcggagcatc atggcatggg agtcgtcttt ggccgacttg
     5581 gtgctgtagg cccgaaggac gtcttcccct ttcacggcgg gggacgcgtc gggatccgcc
     5641 acgatgacca cccccgcctg ctcgacctct tccacggggc cctgctcgtg gcgaatggcc
     5701 ccgatggcgc tgcagatctc ggcacactgg agacactcgg aacacacccc gcactggaga
     5761 caccgggagg tttccgagag tacctgtttt tcgtccagcc ccaaggccac ctcgacgtgg
     5821 ctttccatgc gggccgaaac ccgcctctcg ggcatcttgg ggcgcgccag ggaaggcaag
     5881 tccgggggga tggaaggaaa ctccttttcc cggggtctcc aaggggagga gaccgcaggt
     5941 ttctccccgc tcagttgacc gtgaatggcc cgggccgccg cccggcccga tgccatggct
     6001 tccaccaccg aagaaggtcc ccggatgacg tccccgcccg caaagacccc gggaacgttc
     6061 gttcgaaaca ggtcgtccac ggcgatgtat ccggcatccg tcatgcccac gggttggtcc
     6121 gttcccagca ggaaccgatc tccccgctgg ccgatggcga cgatggccga atcgaaggaa
     6181 aggtcgaagg gcagggcccc ggggaccgga accggccagg gaattccctt ggcatccggt
     6241 tccccaggct gggtccggat gcatcgcaac gactgcaggc ggccgtttga gccggtaaag
     6301 ccgatggttt gggttcgatc gaccaggcgg atcccttcct cccgcgctcc ccgaatctct
     6361 tcctcgtccg cggggatcat ctcctcggga aaccaggaca ggagagtgac ggttcggccg
     6421 gttcgagcca ggccgcgagc caggtcgaag gcggcgttgc cgtcgccgat gaccgcgaca
     6481 tgctctccta ggtcctccgc ctgccctcga tggaaccggc tcaagaactc gagacacccg
     6541 ctcactcctt ccaggtcctc tccgggaact cccagcttgc ggtccgtcca gaccccggtg
     6601 gtgagaagaa tcgccgaaaa ttcttcccgg agtttttcca gctcgtttcg ggggtccacg
     6661 ggatgattgg tcaggaagcg aattcccaat ccctggaggt accgaatctc ccggtccagg
     6721 atttcccggg gcagtcggtg aagaccgatg ccgtaccgca ggagtccccc gagagactcc
     6781 tccttttcga agaccgttac cgggtacccg aagcgggcaa ggtccgcagc agccgcgaga
     6841 ccggcgggtc cggaccccac cacggccacc ttttggggac gggtgggaag ggccggggcc
     6901 gccgcgtcct cggcgtcggc cggggcgtag tcggccaaaa agcgcttgat gtcccggatg
     6961 gccaggggtt cgtccagttc cccgcggcga caggccgact cacagggatg ggaacacacc
     7021 cgcccgcaaa tgccgggaag aacattgtcc cggcgaatga cctcgagggc ttcgccgtaa
     7081 cggccttcct tggccaaggc catgtagccc tggacgttca ctcccagggg acagttggcc
     7141 tggcaaaggg gctgccgccg cttgtcgatc accggacgcc cgggaagact ctggcggccg
     7201 ttgtactgaa tcggtttcaa tcccccgatg gaagtgacgg gacagatccg agcgcagagg
     7261 ccgcagaggg tgcatcgctc cgggtccacg aaggcggggt tgtgaacgat ccgggccgaa
     7321 aagccctggg gcgtatgctt cagggtcttc acttccccgg ggagaacgca tcggattcgg
     7381 ggattccgca agattcgcag aagaccgggc cgaagggcgt agttgagggt gagccccgaa
     7441 gccagccgcc actcttcccg ggcaagcttc ccatgcaggt ccgggtcgga atccaccagg
     7501 accacgggaa tgcccatttc ccccagtttg ttggtcgccg ccaaccccgc gggagtggcc
     7561 ccgatcacca acacacgatg gagtcgatcc ttgggtgcct tcatgcccgg acccccttct
     7621 tcccctcggc cttcttctgg cggcccttga ggatcgcttc cccgtaatcg tgtcccttat
     7681 tgaaggcctg aatgttcatc gcttccgttc ctttggggac cgaatccgcc acggcattcc
     7741 gggcggcttc cacggacagg gcccgggtta ccgcggtaaa gaatcccaac atgacgatgt
     7801 tggccatcat ttttcgtccc agttcctcgg ccatccgcgt ggagggaatg gcaaagagat
     7861 tggcgaattt tgcctgctcg acctgcacca ggtccgagtc caaaagaagg gtaccgccct
     7921 cgggcagcag gggcgaatat ttctcgtaac cgccttgaga catgcagacc aggtagtccg
     7981 gtcggcgaac gtaggggtaa tggatggtgg tgtcggagac gatgacctgg gcgctgcacg
     8041 cacctccccg ggcttcgggt ccgtaggact gggtgagagt gctctccttg cggtccccga
     8101 gactggcggc ccgtcccacg atgaggcccg ccagaacgat tccctggccg ccgaagccgg
     8161 tgatgaggat ctctttccgc gtcgaaacag gattgccctg ttgggttcgg gtgccttgat
     8221 tcatgcttgg actccagttc acgcacaccc accccggtcg gatgccgccg taggaggcgc
     8281 cggacgggag gatcgctcgt atcgttccag gaacgtgggg gattcgacgt ccacgaattt
     8341 gcccagggta atgccccgct cgtagtccat cacggcctcg gccggattgg catcgttgcg
     8401 aataatggtc cgctcctggt acatcctgag ggtatccaaa ggtttttcct tgttgcgccg
     8461 tccgtacccc gtgggacagg gagccaggac ctcgatgaag gaaaatccgc ggcgggtgac
     8521 ggcttcctcc atggacttgg tgaggtcccg ggtgtggagg atggtccagc gggccacgta
     8581 agtggccccg gacgcgaagg ccaggagcgg caggttgaaa gggggttccg ggttgccgaa
     8641 gggggtcgtg gtggttcggg ccatggtcgg tgtcgtggcg gccgcctggc ctcccgtcat
     8701 gccgtagttg aaattgttca cgcagatcac cgtcaggtcc atgttgcgcc gggcggcatg
     8761 gatgaaatga ttcccgccga tggcgaaaag gtccccgtct ccgctgaaca caatgacgtt
     8821 gagctccggg ttggccagtt tcagccccgt ggcgaaggga atcgccctgc cgtgggtcgt
     8881 gtggtaggaa tccagcttga tgtaccccgc ggcacgtccc gagcagccga tccccgacac
     8941 catcaccgtc ttgaccaggt cgatgccgct gtttttcacg gccaccaggc aggaagaaaa
     9001 ggccgtgccg atgccgcagc ccgggcacca gatgtgggga acgcggtccg tgcggaggag
     9061 ttcgtccagg ggatgatcgg atttgggcct gggtcgattc atttagactc cttggatctg
     9121 cttcaaggtc tccaggattt gctcgggggg aatcacggca cccccgctgt gccccaccag
     9181 gaaggcgggc gcccggtctc ccgcgcagcg ttgcacctcg aggtgaatct gtccgaggtt
     9241 gatctccacg gtcaccagcg cccgtacctg ccgggccagg cgccggatct gttcctcggg
     9301 aaaaggccag acggtcacca ggcgcaaaag gcctgcccga attcccatgt tcctggcctc
     9361 gtccacggcg gaaaagctgc tccgggcgga aaccccgtag gacaccaggg caatttcggc
     9421 atcctcgagg cgataggatt ccgtccggat gatctggtcc aggtttcgac gaatcttggt
     9481 cgtgagtcgc tccatcatag ccgcctgggc ctcggcggtc atgacgggat agccctgctc
     9541 gttgtgagtc agccccgtga tgtgaacccc gtaccccgtg cccgcaacgg ccatgggcgc
     9601 cacaccgtcg ggacccgccc ggaacggctt gaaccggtcc ttcctcccct tggggagggt
     9661 tcgattcacc gttcgaatgg ctccggattc cggaatgacc actcgctcat tgaggtgccc
     9721 cacggcctcg tcggtcatga tgagcaccgg ggtccggaac cgctcgctga gattgaaagc
     9781 cgtgatggtc tggtagaaaa gctcctgggg agaagccggg gccagggcga tgatttcgta
     9841 gtggccgtgg gacccccagc gggcctgcat catatctccc tgggccccca gggtcggcag
     9901 gcccgtggaa ggtcccgccc gctgaacgtc cacgatgacg cagggggtct cggtgcagac
     9961 ggccaggccc aggttttcca tcatgaggct gaatcccggg ccggaagtgg cggtcatgct
    10021 cttgaccccg gcacaggacc ctccgacgat ggcggccatg gcggcgattt catcctccat
    10081 ctggatgaag acccctccca cgtccgggag gcggtgggcc atgtgttcgg caatctccgt
    10141 ggcgggggtg atggggtacc ctgcgaagaa tcggcacccg gccgccaggg ccccttcggc
    10201 gcaggccgca tctccgctga taaaatgttc accggtcaag actgcaggtt tcatccgcga
    10261 accttcctta aaaacccgac gtttccaggg atgcaagggg atgagggttc attgggcggg
    10321 agccggggcg ggatcgctgg ggaccgcttc caccgaaaag atggcgaaat ccgggcagat
    10381 gatttcgcaa taacggcagt tcacgcaaag gtccggcttc ttcacccgag gaggatgata
    10441 gcccttgttg ttgaacttgg cggaaatctc gaggacgtcc cgtgggcaat attcgatgca
    10501 gtagccgcat cccttgcacc gttcctcgat gatatggacg atgcctcgag tcacctggat
    10561 gaggttggtg tccagcggag ttcgccaaaa cttcatcgtc gccggtccct gcccgtttga
    10621 gggcatatcg ttcaagggtt ggaaaaaggg ggccgcgccg catggaaccg cacggccggg
    10681 gcaaaagccg aaagacacga aattgtatgc aatccgggaa gtaagtcaag gtctattcgg
    10741 gcagcggaaa ggattatttc gagacaccgg tcgcaaaaat ggggggagta tctgcaaatg
    10801 agagcccagg gtatggagag gaacgggtcg gtctgccccc cttccgggta ggaaaagagg
    10861 cggaacgaga gggcaaggaa ccgaatccgc cgggggtggc ctccaatgcc ccggaaaacc
    10921 cccgggagga ttccctcctg gactctggaa cgtggggaga gccggttcgc ttctccttcc
    10981 tggggtcgtt gacttcctat atggaccaga tggatcccaa agattttctc atgatccccg
    11041 ggtcggggga gagggaatgg gatgggaggt cgcactgagg ggactctttg atcgttccaa
    11101 aggatggaag cgcagggggc gggacggggg cccggcgggc aaggaggcat cccctccctg
    11161 ccaaagcccg cggggggcaa tcaggccagc ttccggccca aggcgaaggc cttctcgaaa
    11221 taatgctcca ccccccgatg ttccacggct cccgcgtaca tgtcccccac ggtcttggcg
    11281 ttcaacgaat ctttgagggt ctcccggatc agggccgtgg cttcatcgca gaacctccgg
    11341 tagatggggg gaacgatgcc ggaaaccacg atgatgacgg ccttgcgcct cttcttggac
    11401 cgcggcacgg ggcagccgcg gagggtgaag acccgcccct cgggcttggc gaaggtccag
    11461 cagatccgtt ccaggaaagt catcatcagg gcggtgacgg atcccatgtg gacgggggtc
    11521 cccacaacga ggaggtccga cgccaaaaga tcttcccgga tgccgtccat gtcgtcccgg
    11581 aggacgcacg gggccatggg tccccgggtt ttggcgtccc gacaggaaag gcagtttcgg
    11641 cagtacccga tgtcgtagtc gaccaggttg atctttttca cccggaggcc ttccgtcccg
    11701 gattgcacac cctccaaggc tctgtccacg agccggtccg ttgccttgcc ctttctcggg
    11761 ctaccgacga ttgccaccag gttcattccg tcctcccaaa gggggcagcg gacggttcgt
    11821 cccccgacgt gaatcttccg ttgcgcggcc atggacgggc aacgaaaggc gtccgcttcc
    11881 attcggcttg acccgtccgg tacgggcagg cgcaaaccga atccacgttg ctagaatccc
    11941 ctgaaacaaa taccgggtcc cggtcctcgt tcaaattcga ggcgtccgtc cggtgggagc
    12001 cgcctttcat gttcggccgt tgacaaaaga cggggtccgg cgcgggaggg ccgtatccgc
    12061 gggaaccgtg aatggggatt ccatcgggcc cgccggacgg atccgagggg gagggtctcc
    12121 ctcgttggag tactaacgac aaccaatcaa ggtagcgggt gaacaggtcc ggcggcagag
    12181 cgacattgtg atcgggatgc agatcgtttt cctcgaccgt ccccgaccca cgatcccgca
    12241 cccgattcgg ggttttgggg gaagtcgagt tgcaggcaca gggggcgggc ccggccgaac
    12301 ccccgggttc ggcatttccg cccggccccc catcaagaaa ggttcacccc ggccgaggcc
    12361 agggcccgga gagcgtcaag aagtccgggt cccggctttc gccgaagagg gattgggagt
    12421 tttcgtcagc ggcattttct cccctcgagg ggggaaacgg catcggttct tgaggctatg
    12481 cctcatacgt tgcaatgagg acatccggcc ggttcttcct cctttttctt gccgtcttca
    12541 ccttctttgc cgtattcctt cacgatttcg gcgatcttcc tcaaccggtc gtccaccttg
    12601 cggaagaccg ttccctcggg ataggtgccg tcttcctgga gctttccggc ctcgaccccc
    12661 gtgaggatct cgatgccctc ttcaatggtg gtgacgggcc agatatgaaa ctgtccggcc
    12721 tcgacggcgt ccaccacttc ctgcttgagc atcaggttgt ccacgttttt cgaagggatc
    12781 atgaccccct gtttccccgt aaggcctttg aacttgcaga tgtcgaagaa gccttcgatc
    12841 ttgtaggtgg cgccgccgat gggctggacc tcgcccttct ggctgacgga gccggtgacg
    12901 gcgatcccct ggaaaatggg aatgccggac agagcgctca gcagcacgta gagctccgtg
    12961 ctcgaggcgc tgtctccctc gaccatgccg tagctttgtt cgaaggaaag agtggccgta
    13021 agggagatgg gcttgttgtg ggcaaagcgt tccttgagga agctggaaag ggtgagcagg
    13081 cccttggtgt ggatgttgcc gctcatcttg gattcccggt cgatggaaat catcccttcc
    13141 cgtcccacgg aaacgttggc ggtaatgcga ttgggttttc cgaagacgtg atcgccggtc
    13201 atgagcaccg agaggccgtt cacctggccg accttatagc cgtcggtctc cacccagaag
    13261 atgtctcgtt cggtcagatc cttgattcgg tcctcgatga ggttggaccg gtaggtgcgc
    13321 ttgtcgatgg cttcctggac gtgtttgcgc tggatgaatt ccgcctggtc cagccccgca
    13381 aagtagttgg cttccttgag caggtcggaa atgctgccca tttcgaggct cagcttcttt
    13441 tgatcctggg tgagttccat gctgtattct atcacccggg acacgcccga ccggtccagg
    13501 tggcgcagtt gatgttcctt gcaatagtgg ctcatcatcc gggcgcactc gagaatcttc
    13561 tcatccttgc ggtccatctg gtcgtccagg tgggccttga ccttgaagta cttctggaac
    13621 cgctcgtcgt aggtgtagag cagctggtag atgtaggggt cgcccgccag cacgatcttg
    13681 cattggaggg gaatgggttc cggacgaatg gtgcgggtgc tgaaaagacc gtaaagctcc
    13741 cccaggtcct cgatcttgat ttcctggtcc cggagggccc tcttgagggc ttcgtacgtg
    13801 atgtaccatc gcaggaggtc cagggctttc atgatgagat agccgccgtt ggccttgtga
    13861 agggctccgg gcttgatcat ggtgtggtcg gtgaagaggg ctccgaacca ggcctgccgc
    13921 tcgatggatc cgaaaatgtt gggataggcc gggttggatt cgatcaccac cggggcgccc
    13981 ttggtctcgg agttgtcgat gaggacgttg acctcgtact tgcgcaagga ggcctcacgg
    14041 gggggcatgg ggaagggagc cccgggctgc tgttgctgtt gggcctcggg cttcttcttg
    14101 aattcctcga tgttttcgag gatgtcttcc tgcaccatct tgaaatattc gaggacctgg
    14161 ggctcgtcct ggtacttttc ctcatagctg tccatgagtt gcccgaccac gaagagggca
    14221 atctcgctgt caagcttcga atgcttctgc ttgaattcct cctcggcctc ccggatcctc
    14281 ttgatggctt ccttcatctt ctcgtggagc tggtcgctct tttcccgcag gaccttgcgg
    14341 tcttcctctt cgaggtggcg caggtcctcc tgggtcatgg gttcgccttc cttgttggcg
    14401 ggaatgatca ccatgcccac ttgggaaaac tggaggataa agccttcggc cttggcctgg
    14461 gcggacagct cgtcgatgat ctcgcgccga agccgctcga aggactggtg gacctcgctt
    14521 tccttggtcc gatagtcgtc gctgtcgaaa acctcgggga tctttgcctg cagggtgttg
    14581 atgaactcct gcatgtcctt tctcagcact ttgcctcgtc ccgccgtgag cttgaggctc
    14641 ttgggcttgt cggtttcctt gaagttgtag acgtagcacc aatcgggggg ggtcggttct
    14701 tccttggcct gttcctcgag ataggtcttg ccgatataag taaggccggc cttgggaggc
    14761 ccggcaatga agatgttgta gccggactct ttcatcccca tgccgaactt gatggcgtcg
    14821 atggcccgct cctggccgac gacccgctct tccaaggctt cgagggatga ggtgttttca
    14881 aagggaaggg aatcaaggtc gaccgtggct cgcaaatctt tggccgatag ctcagcaaat
    14941 gccttcgaca tcgggatctc ctctgttcaa gggtgcagtt cctggtgcgc aggggggtac
    15001 ccggcggctc aactgacgac ctccagcttc acccgtttct tatggctgga agcggctgcc
    15061 tgcaaaaccg ttcggatggc gttgatggtg tttccgttct tgccgatgat tttccccagg
    15121 tcttcgggag atattttcaa ctccagcaaa acggtgtcct cggcttcctg ctcgttcaag
    15181 aagacttcct ccggatggtc tgccagtgat ttcacaagat attcaactag ttccttcatt
    15241 ttacccgttt cctcttcctt ccttatggcg atgcggtgca aggccccgcc ttgcctttag
    15301 agcttgtcca tgacggaccg caccttgagc tccgggctgc cggggcggtc gcgacgatcg
    15361 tcgatcttta cctgggtgag cacacgggga acccctcggg cgaagcagct ctcgtgcatt
    15421 tggcgaacga ccttccagac ctcgtccagg tcaccggaga tgatggtgcc catggcagtg
    15481 agctcatagg aaagaccgct gtgcttcaac actttcaggg ctcccgccac atagtggctg
    15541 agactcgtat cgggcacccc caggggaacc acactgactt ccacaatggc catggctagc
    15601 ttccttcccg aaaagaattc caaccggcgg cggaaagcat ccggttcaga ccccggagag
    15661 ttcccgccag gtgctcctcc cggtgcggct cgagggtcaa cacgggtttc ataccgcggg
    15721 agccgagaaa ccggaacagc ccgacgaaat cgatggtccc gtccccgatg ggaagatggg
    15781 cgtcgccgga accgtcgttg tcgtgcaaat ggacctccct caagcgatcc tgcaactcat
    15841 cgagccaggc ggtcaagggg gtcctcgaaa agctgtgctg gtgacccacg tccaggcaaa
    15901 agccgaaata cggggacgga atgcgtccga agagctcctt gtggaggtcc ggtccgtatt
    15961 cccagacgtt ttccaggagc aaaggcacct ggaggtcctc tcctcgacgt accaggattt
    16021 cccacacggt caggctttcg tccagccata gggatcggtg gccgccgtgg tgcctcgggt
    16081 cgaagcccgt atggcacacc acctggatgg gccggaagga atccacgacg tcgaagaact
    16141 gttggagacg gcgccgagac gcccgtcgaa tgagcgggtc gacgctcccc gggctcagat
    16201 cccaaaaggg accgtgcagg gtgatgcggc acccttccct tcgcaattgg ctcacgaccg
    16261 cgacgatctc gggacgggaa agctcatcaa gaaccgttgc ctcaatccct atttccacgt
    16321 ttagccgaag atgcagaata gtgtccacat atttcggcag ttctctccaa ggtatgttca
    16381 cgtggacgga tcgcaagagg ctctgcagct tttcttcggg ttccggaggc actcggactc
    16441 tcttcctaat cgcgggtggc ggtttccacc ctgaaataac cggcacggcg cctattcatc
    16501 gccatttata gtagaaattg gtggattgga cagtaaaatt ctgccatgct ggcggacaaa
    16561 accggaaagg acgacgaggc tcatgaagca gatagtctac gatcaattgc gggaactcga
    16621 ctatttcaag attcgggact acctggacga aaacgtggaa aagggaaccc tggaagggat
    16681 ctaccaggtg gatctgcccc cgggccttca ttcgagaact caggccgagc atgcccggtg
    16741 tcaggggttc tactttgccg taaacctcga tcgaaaccag gtagccttcg aactgttgat
    16801 tcggagccgc caggtccttc gatgcagctg catcgggtac gcaacccccg agcagaggga
    16861 ctatattttt gcctttgccg accaggtgct cgaggacctg ggaatccgga tctgatctcc
    16921 cctgctcccc tcatcccacc ttttcgggga acgggtgaag gaggaagtcg gccaacacca
    16981 acatcaccat ggcttcgcat acgggaacga tgcgggggat ggccgacacg tcgtggcgtc
    17041 cccggatgcg gatcttcgtg ggtttgccct cggcggtgat ggtctgctga acctttgaaa
    17101 tggagggaat gggcttcacg gcagctcgaa gaacgagatc ggcccccgtg gagatgcctc
    17161 ccaatacccc tccggcatga ttggattcgt agccctcgac ggtaaggggg tcgttgtgct
    17221 cgctgcccag catgtccgcg gcggcgaagc cctccccgac ctcgactcct ttgacggccc
    17281 cgatggacat gagggccgcg gccaggcggg cgtccagctt atcgaagacc ggctccccca
    17341 ggccgggggg gcagccctgg gctcgaacct cgacgatgcc ccccaccgag tctccggcgg
    17401 ctcgcacttc ggccagccgc tcctccatgg cttttgccgc ttccatatcc gggcagtaaa
    17461 ggagattttg gtcgacggcg tccgcgtcga acctgcggca acgaatgccc cccagggata
    17521 tggtgtaggc ctgcacccgg atgtcgtact gccggagaaa tttgcgcgcc acggcgcccg
    17581 cggccacgcg cgccgcagtt tcccttcccg agctgcggcc tcccccccgc caatcccgga
    17641 cgccgtactt ctgatcgtag gtccggtccg catgccccgg gcgatagacc atggccaggg
    17701 gctcgtagtc ccggctccga acatctcggt tgcggatggt gaggctgatg ggagtaccca
    17761 tggtgactcc ctcgaagacg ccggaaagga cctccacccg gtcggtctcc tgccgggagg
    17821 tggtgaggcg ttttcccggc ctgcggcgct cgaggtcttt ctgaatgtcc tcgacggtca
    17881 gggggattcc cgggggacat ccgtcgatga tcacccccag tcccgggccg tgggactccc
    17941 cccacgtggt gatgcggaac aaacggccaa acgagtttcc tgccatatct gtgctccgga
    18001 ttcgcaataa accttgccca aatgcggatt cttgacactc ttgaggaaaa agttatcctt
    18061 taattctggg acaa
//
LOCUS       DAOOVC010000072        17899 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2157_length_17899_cov_5.964302, whole genome shotgun sequence.
ACCESSION   DAOOVC010000072 DAOOVC010000000
VERSION     DAOOVC010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17899
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2157_length_17899_cov_5.964302"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLM79_10390"
     CDS             complement(<1..796)
                     /locus_tag="PLM79_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86761.1"
                     /translation="MKDYLFTYPRRNQILLLLGDCLALGLSVFFGYSMKIVSEHSTFA
                     FDLLFSKLHPLHFLVVPVYLTTLHLMDHYDLDRHWDLPRNLLKLWGSMFLAGCVVSGL
                     LFFTPKYFFGREALVFQVVLALLLVTLWRVIAWSLLLRKSPPTRLAVVGHGQIISGFI
                     EDLSRETNSGIRVSSICVTDSKNPGACLQPRSVTTHRNLGELLQNKEFDALAFDSTNG
                     YFSNEEVREILRLKFEGKAVHDLSILYENMTGRVPLTYIDGQWLLKS"
     gene            complement(1132..2055)
                     /locus_tag="PLM79_10395"
     CDS             complement(1132..2055)
                     /locus_tag="PLM79_10395"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOV86762.1"
                     /translation="MIPLLYIAGYGRSGSTILEAMLGTDPRVFPTGEMSRFWEMVQDP
                     SILCSCGEPVQSCPLWGRVLQRVLPDSPPPGSLRKLMRRVEGSRTKTGRPSTDDLRTY
                     ERITRRLFSALTEQLDSLVRVLVDSSKTAYGHCNRPGMLHRLFPESEVRVLHLVRDVR
                     GVAFSMRKGLNRNLERNGKGGWNLLNFFRAIIGWTYANMRAASLKKTLPRGRYLLVRY
                     EDFVLSAPAVLDRVRDFLGLEPPDGLSDPVFLQGRFERHQLAGNRNRFDATVAIRPDF
                     RWKKELKPLEKALALGLGWPLMKRFDYLGGF"
     gene            complement(2124..3341)
                     /locus_tag="PLM79_10400"
     CDS             complement(2124..3341)
                     /locus_tag="PLM79_10400"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV86763.1"
                     /translation="MLRNAKIKVLYVTNTDWGLYNFRLAVLQALRERNFEVSAAAAKG
                     AFWSKVRGLDRLVELRHYRKSVHPMSDIRLVKEIRTLLLEEQPDIVHFFSIKPTILGG
                     LMARPLGVPIVVHTIAGRGSLFSMEGWGAKLLRVLMVNPLYRLVGALSDLIFFQNDDD
                     RKGFLQQGMIPGSKAGLVPGSGVDTDYFSSKRVDPAKLDRLRKELGIGREATVVTLIS
                     RMLYDKGIGLFVAAAREIRNRNSMVRFVLVGPHDLENPSGIPLERLEEWNREGIVSYL
                     GRRPDVRELLALSDIVTLPSHYREGVPRVLLEAAAMGKPVVTTDMPGCRDAVQDRKTG
                     LLVPPKDPVQLTRAIQTLIEDPELRDRFGQAARKLAEDRFEIGRVIDITFRTYCDLLR
                     KKGYPSQAIPGTD"
     gene            complement(3534..5096)
                     /locus_tag="PLM79_10405"
     CDS             complement(3534..5096)
                     /locus_tag="PLM79_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HOV86764.1"
                     /translation="MRRLVGVGVAAMGLSLAMACSVLAEIVARPLEEKPRIQSSAPPW
                     STEAKPRPLVLVQGEAEGMILAVRTSDPELALLFSQEFGQGLRLRVYEVRTLNLPGTG
                     GFGDPLVPAAPGAKVRASSGLIHLYVKIEASRGAAPARGEVALRLRGAKEEKEFRVPY
                     EVLPFVLDPAAIPFVQATIRPLFDTYPRKVAAEERQQIFKNMTALLVRDYAVRSFAGL
                     GGSGKTDLTLDKGRDTFARYLEMVRFCLDDLGVEKMRIPAGQFFTKRKSLADVYRPVS
                     EEAIMASLAQHRKNMQAIAENPRWKGKLSMRLWDEPKPSQYPEVVLLYKAARKVFPEF
                     SLELTEQPDDALLDIADVYIVNPRWFDEKQVSRAQRKGSQVWIYCNKASGIDEPLKGM
                     RIIGWLIWRYRLDGYLFFSMNYWDEDPWKQSSRMYRGTFLYPEHQGQNVYPSLRLEMF
                     REGMEDAAILKQVESLAEKSRDTATQKMLEDFRRTYVMKEKHGGSQDPVGPREALMKR
                     IGEASGTWGGRP"
     gene            complement(5104..6363)
                     /locus_tag="PLM79_10410"
     CDS             complement(5104..6363)
                     /locus_tag="PLM79_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86765.1"
                     /translation="MIDQQRLGFSEKILHGIILLFFLTVPVYVLPSGGFQLVDIPIIF
                     GMVLASLVLNRELYDRSRLIVMLLAVYVSWTVLINIVYFVGRSDLFYMKSAIQLVFIV
                     FMFAAFVVLLQRLILKKKGVLCIYAGLFLSLIPPFLVTGSYDTTDTEGVGGVSMRTAL
                     SFNNPNQLAYFAILILAIFFLIRLYSRRFEFTRWQRIGTGFVAVAVWVMSHAFVFLSA
                     SRAGLICIGVLDCLIFWKLRRKVVIAGTLLIVLLLPAGVIFRNDLPQVDILVVQRLMM
                     TDTEAAMSKRVGTRFFQSLASEYAAMFGSGGVSSNPKVIEVHNAFADMLLCYGMIGVA
                     IYLFFLSAVAVPYLRFMRRYEDLYYLGILFAIMLYNVGHNGMRTRLYWVFLALWFAVT
                     REVIRERSQEIESPSRAERLVAADAPV"
     gene            complement(6366..7931)
                     /locus_tag="PLM79_10415"
     CDS             complement(6366..7931)
                     /locus_tag="PLM79_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOV86766.1"
                     /translation="MTHTQSDAAPPESAGEKGASRDIHKVAKGTGATLVGTAFGKSIF
                     FLSQILIARLLGVEEFGLYSLGLAAVKICEIFARMGLNMGGMRFVSIHKDHDLPKLKG
                     VLISATTLSLVGGCFVGFPVFFLSDFMAGSVFGKPELAPVLQSFAFAVPFTAGTMVVS
                     SLLQGFHTTKYTMISREIMQPALNLVLFIAFYLTGFGLLGAVYAFILSNLGALLTGVY
                     YCRKLFPDLFRKHLPAIYEIGSLVSYSVPLLFVGFLHFLLSSADILMLGYLSSARDVG
                     IYRAAAQISSVMSVFLIATNSIYAPVAADLYQRSEMARLAGIFKTTTRWLCFATVPIF
                     ILLLFSGREILMIFGKDYAESSATTALSILAFGHLVSCMTGGVGVTLTMTRRQKLELF
                     NSLCMAGLNLSLNYLLIPRYGAIGAAIASSSAIVAINLLRLGQIVLLYRMHPFTREVL
                     KYMVPAAVSVLVLFLYYQMHLPAQFVVSLLIKTAVILVLFAAFFLTGQCMAEEDDIVW
                     KRISRYFRRVGSH"
     gene            complement(7928..8785)
                     /locus_tag="PLM79_10420"
     CDS             complement(7928..8785)
                     /locus_tag="PLM79_10420"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOV86767.1"
                     /translation="MTLPNFLGIGAQRAGTTWLHAQMRSHPEVYLPRRRKELHFFDRY
                     YERGLEWYETCFPRADQSPGVRWMGEITPMYLYDPRVPRRIKEHLPDCRFLAILRNPA
                     DRAYSQYAHHVQNKGEERNFRDFLAAEEDVFGRGLYYRQLRRYMDLFPGENFLVLIFE
                     EVMKDPAGALGELANFLGIQPGGFDSNLVGRKVNPSYRSRFGKVGSVVRNWGEFLRRS
                     DLDWVINAAKALGLRGIFGNAGELPRMSPEVRRELLERYDADMAALEGLLRKDLSVWR
                     NPVSQDRGR"
     gene            complement(8958..9962)
                     /locus_tag="PLM79_10425"
     CDS             complement(8958..9962)
                     /locus_tag="PLM79_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOV86768.1"
                     /translation="MRKKVFILGFGRSGTTWIADIVSKASGTLVLFEPFHPSVTEASR
                     EVSYAPVGKNGNSLKEYLNRVMGKRHRKMWLMRNHVPVRLEEISPNFLELIWENCGIL
                     GFKEIRANFMLSWLHDNFDAKIVYIIRHPCAVVSSILKRQNFWEFGWPGTYELFLAKT
                     VYHDHYKSHRIARCLPVVEQAKTDLERIAVMWAITHAVTLPELKDLNLPLFYYEDFYA
                     RPFPAVKELMAYLEMPEIDLHPAHIFTPSMTTLKTLHGLYGMDKSVSREGASLFWKGV
                     LDERKVGTVLEIVRYFGIELYNETGFPLKEGFTGGDGRDMVPLSDQFPGPSGHEIFRF
                     "
     gene            10543..12012
                     /locus_tag="PLM79_10430"
     CDS             10543..12012
                     /locus_tag="PLM79_10430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOV86769.1"
                     /translation="MLFNSYTYLVFFLPITAGLYFLLSRRERHGEGKILLILASLGFY
                     GYHEFRTLPVLLASVLVNYFSCTLFNAVAPRRRKPLLVALLLFNLGLLVYYKYSGFLV
                     SNANSLFHTGFSMPNLLVPLGISYLTFLQIAYCVDSYRGVVRNNSFLNYTLFAVFFPK
                     VISGPIAHHNEILPQLDNPSTKTFSWQNLSTGLFLLSIGLFKKVVVADNLAPYVNFGF
                     DQAQSLNFFSGWATSLGYTLQLYFDFSGYTDMALGSALFLNLKLPLNFASPYKSTDIR
                     QFWQTWHITLGRFLRDYLYIPLGGNRRGKLCTYRNLLLTFLVCGVWHGAGWLFFLWGF
                     LHGIAIVVHRAWNQTGIRMHRVPAWFLTFNFVNIAWIFFRAREWEDALKVLKGMVNVQ
                     SIGWEGVQLRQFFVYYLKDHQRHALEILAGVQGGEAMILLVMLCITAFVIVAGNSNRY
                     LENFTPRFRHVCFFVASFLFFVICNTYLAVRSEFIYFQF"
     gene            11987..13039
                     /locus_tag="PLM79_10435"
     CDS             11987..13039
                     /locus_tag="PLM79_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86770.1"
                     /translation="MSSSTSSSRDPASHRRFVLLSLSFLLLAVGLNFLLCEVFARKVI
                     WPRKCFQLLELQRDGRTFRTVSLGTSHTAVMNFDYPGLFNFGLSRTFPSIMYFKMKYL
                     AKAAPNVRTLLLEADTHLFYGLAAHCDKYQHSVYIDEDVDPSLGNHPRGSKGEFLSLK
                     EEIAPTVYKQMAMSLHRLLTSGKNSSEKTARSAETEDVSEDVRWDLLPREVREQTAIS
                     RLKHFSLYDYEPMNPLLVEYYEKTIRMALNQGMRVIFIRYPLTNEFLAHHNPQIEDEV
                     SAFLGALSKKYGIPLLDYRKVFVDRQDLFSNQDHCNGEGKKVLRERILADLRGLGVSM
                     VSAAAGEGSPDGPQNP"
     gene            complement(13359..13991)
                     /gene="cysC"
                     /locus_tag="PLM79_10440"
     CDS             complement(13359..13991)
                     /gene="cysC"
                     /locus_tag="PLM79_10440"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOV86771.1"
                     /translation="MNIYPFKPTIQCADRHQLNGHRSALLWFTGLSGSGKSTLAHAVE
                     ARLHKFGIRSYVLDGDNIRSGLNRDLNLSPEGRKENIRRVAEVAKLMVDAGILVFSAF
                     ITPYGESRRFIRELMKGWPYFEIYVRCPLEVCEKRDPKGLYAKARRGEITDMTGIGDT
                     YEEPESPDLVVETGVQTLEESTEQIVGFLIENRIIKMPALRGDLPRTKAC"
     gene            14374..14667
                     /locus_tag="PLM79_10445"
     CDS             14374..14667
                     /locus_tag="PLM79_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOV86772.1"
                     /translation="MGAHAKEAVGLEQEFIISNKLGIHARVAAQIVKVAAQFQSEIWI
                     MKSGNRVNGKSILDVMTLVCPHGSRIRVSVDGPDAAAAMDALAHLFQTKFGES"
     gene            14901..16724
                     /gene="ptsP"
                     /locus_tag="PLM79_10450"
     CDS             14901..16724
                     /gene="ptsP"
                     /locus_tag="PLM79_10450"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOV86773.1"
                     /translation="MNPSSEVIEGIGVSPGIAIGKAYLVEQGRIPIPHHTIEDESGVD
                     RECQKFQDAVNKAEEDLEEIKARILPVIKEHARVLEVHQMILRDPLIFGETLRLIRQE
                     RLNAQWALQRSLLKAYELFESVEDEYVRSRTTDIQAVGERILRNLAGKEENLFNSIRE
                     RVILVAHDLSPADTAQLLLEKTLGFVTDMGGRTSHTSILARSLNIPAVVGTERATQAI
                     PSGCLVILDGSAGTVIIDPSDEEIGSYYERQIQLEEYQKSITRTAHLPARTRDGHLVR
                     VEANIELLEEVVAVKDNGAEGIGLYRTEFFLINRKNFPDENTLYSNYRELAELMAPHW
                     VTMRTLDFGADKLGAWYPKMEETNPALGLRSIRLCLHYRDLFKTQLRAILRASASTKN
                     VRLMFPLISGVAELLEARGVLREVREELRRERIAYDEDMPVGVMIEVPSAVAVSDLLA
                     REVDFFSIGTNDLIQYSIGIDRANEHVAYLYEPLHPGVLRLIKMAVEAGHRAGIPVSL
                     CGEMAGESFYVPILLGLQLDCLSMNPQSVPRVKNLISRSLLKDCRRFVKRILESSTAK
                     EINELLQEMMLKNFPEEYRLFDPRALSPRILSKRRRVPQRL"
     gene            16770..17258
                     /gene="smpB"
                     /locus_tag="PLM79_10455"
     CDS             16770..17258
                     /gene="smpB"
                     /locus_tag="PLM79_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOV86774.1"
                     /translation="MAKEAKNSERSPIRLVCRNKKAYFEYEILETFEAGMMLLGTEVK
                     SLREGRANLKDSYARIKKGEVFLYDLHISPYPHASYNNHEPERVRKLLLHGFEIKRLQ
                     GKTQERGLSLIPLKIYFSHGRAKAELALARGKKLHDKRESLKKKDEARELQRLRRRDK
                     IS"
     gene            17286..>17899
                     /locus_tag="PLM79_10460"
     CDS             17286..>17899
                     /locus_tag="PLM79_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOV86775.1"
                     /translation="MKRTSVPRSKVAPMRCLTLTAPHSLVLTDRPRPQPAEDEVLLQV
                     SHCALCRTDAKMWQQGHRDLLLPRVLGHEFCGSAVGTGRRFVVWPGRACGQCDDCLQG
                     RENLCPSMRILGFHRDGGLAEFVTVPASALIPISDDLDPAVACLAEPLACSLNGLARV
                     RVQEGERVLIVGGGTLGILLALGAVSRGARVCVVDRNPSKLHRSR"
BASE COUNT         3880 a         5358 c         5073 g         3588 t
ORIGIN      
        1 ggctcttcaa gagccactga ccgtcgatat acgtcagggg gaccctgccg gtcatgttct
       61 cgtagagaat cgagaggtcg tgcacggcct ttccttcgaa cttgaggcgg aggatttcac
      121 gaacttcctc gttggagaaa tatccgttgg tggaatcgaa cgccagtgca tcgaactctt
      181 tgttttggag gagctcgccg aggttgcggt gggtggtgac cgaacggggc tgcaggcagg
      241 ctccggggtt cttggaatcc gtcacgcaga tactgctcac cctgatcccg ctgttggtct
      301 ccctcgagag atcctcgata aacccggaga tgatctgccc gtgtcccacc acggccagcc
      361 gggtgggagg cgacttgcga agcaggaggc tccaggcgat cacccgccac agggtaacca
      421 gcagcagggc caggaccacc tgaaaaacca gggcctcacg accgaagaag tatttgggag
      481 taaaaaacag cagacccgac accacgcatc ctgccaggaa catactgccc caaagtttga
      541 ggaggtttcg aggaaggtcc caatggcgat ccagatcgta gtggtccatc aggtgcaggg
      601 tcgtgaggta gacgggaacc acgaggaagt gaaggggatg aagtttggag aagagcaggt
      661 cgaaggcaaa ggtggaatgc tcactgacga tcttcatcga gtacccgaag aacaccgaca
      721 gtcccaaagc caagcagtcc ccaagcagga gcaggatctg gttccttcgc gggtaggtga
      781 ataggtaatc tttcatggta ccattcttcg gtaggtccgg gtttgcgggc cccggagcgc
      841 gtcctcgtgc cgacaacaac gcccggtgcc tccccgggcg gttccggacc cggcctcgag
      901 atgccggggg aaagccctcc gcatgggagt tgcccgaacc gatcttcccc tcggcgagag
      961 ccggaatccg ggcctatccc gcgactcccg gcaccccttc ggctctggaa ttcgcccggg
     1021 gaacgatctg tttcatgcgc aattcgggga gcaccttcgt cagcgtggcg ccgtaccgag
     1081 tgttcgcccg cacccatgga tcgaggacca cgcccgtgaa ggaccgccgc ctcagaaacc
     1141 tccgaggtag tcgaaacgtt tcatcaaggg ccaacccaag ccgagagcca aagccttttc
     1201 caggggcttt agctcctttt tccaacgaaa atcgggccgg atggcgacgg tcgcatcgaa
     1261 ccgattccga tttccggcca actgatgtct ctcgaaccgt ccttggagga agaccggatc
     1321 cgaaagaccg tccggcggct ccagccccag gaaatcccgg actcggtcca acaccgcagg
     1381 cgcggacagg acaaaatctt cataccggac cagcaggtag cgtccccggg gcagggtttt
     1441 cttcaaggat gcggcgcgca tattagcata agtccaaccg atgatggccc gaaaaaagtt
     1501 cagcaggttc cagccgccct ttccgttgcg ctcgaggttg cgattcaatc cttttcgcat
     1561 ggaaaaggcg accccgcgca cgtcccgtac caggtgcagc acacgaacct cggactcggg
     1621 aaacagccga tgcagcatgc caggacggtt acagtggccg tacgcggtct tggaggaatc
     1681 caccagcact cggacgagcg aatccagttg ttcggtcagg gccgaaaaga gtctccgggt
     1741 gatccgttcg taggttcgaa gatcgtcggt gctgggtctc cccgtcttgg tcctcgatcc
     1801 ctcgaccctg cgcatcagct ttcggaggct gcccggcgga ggcgagtccg gaagcaccct
     1861 ctgcaaaacc cttccccaca acgggcaact ctgcaccggc tccccacacg aacatagaat
     1921 ggaagggtcc tggaccatct cccaaaagcg gctcatctcc cccgttggaa acacacgggg
     1981 atcggttccc agcatcgctt ccaatatggt ggatccgctt ctgccgtagc ctgcgatgta
     2041 caataacgga atcatgggcg ggaaacccga actcctttcc cggtcttgac cgaaggggcg
     2101 cctccgggga caattgacct tcgtcaatcc gttccaggaa tagcctggga cggatagccc
     2161 ttctttcgca acaggtcgca ataggtccga aacgtgatgt cgatcacacg accgatttcg
     2221 aacctgtctt ccgccagctt tcgcgcagcc tgcccgaagc ggtctcgaag ctccgggtcc
     2281 tcgatgaggg tttgaatggc ccgggtcagc tgcacgggat ctttgggagg caccaggagt
     2341 cccgtcttcc ggtcctgtac cgcatccctg cacccgggca tatcggtggt gaccacgggc
     2401 tttcccatgg ccgccgcctc gaggagcacc cggggcaccc cttcccggta gtgggacgga
     2461 agggtcacga tgtccgagag ggccaacaac tcccggacat ccggacgcct gcccaggtag
     2521 gagacgatcc cttcccggtt ccattcttca agccgctcca ggggaatccc cgacgggttc
     2581 tccaggtcgt ggggccctac caaaacaaaa cggaccatgg aattccggtt ccggatctcc
     2641 cgggccgcag ccacgaacag gccgatgcct ttgtcgtaca acatccggga aatcagggtc
     2701 accacggtgg cctcccggcc gatccccagt tcttttcgca accgatcgag cttggccgga
     2761 tccacccgct tgctggagaa ataatccgta tccaccccgg accccggcac cagcccggcc
     2821 ttggatcccg ggatcatccc ctgttgcagg aagcccttcc gatcatcgtc gttctggaaa
     2881 aaaatgagat ccgacaaggc ccccacgagc cggtagaggg gattgaccat aaggacccgg
     2941 agcagcttcg ctccccagcc ctccatagaa aagagactcc ccctgcccgc aatggtgtga
     3001 accacgatgg gaacccccaa aggacgggcc atcaaaccac ccaaaatcgt gggtttaatg
     3061 gagaaaaaat ggacgatgtc gggttgctcc tcgagaagaa gcgtgcggat ctctttgacc
     3121 aggcgaatat ccgacatggg atgaacgctt ttgcgataat ggcgcaattc gaccagtcga
     3181 tccaggcccc ggaccttgga ccagaaggcc cctttggccg ccgccgccga aacttcgaaa
     3241 ttcctttccc ggagcgcctg cagcacggcc agtctaaaat tgtagagtcc ccagtcggtg
     3301 ttggtgacgt aaagaacctt gattttggcg tttcgtaaca aattcccgtc ccgtcgatga
     3361 aaggtgggcg gcggcctccg cctcggagaa tccggttttt cccccggccc ggccagccgc
     3421 cccgaaaaac cgggtcccga cgtttaccgg tcaggcatcc tgaatactcg acacagaaga
     3481 ggcctgtcta ttccgtttct tgccgtctgt ccaccctatt cttgggcgga acttcacgga
     3541 cgcccccccc aggtgcctga agcctcccca attctcttca tcagtgcttc ccggggaccg
     3601 accgggtctt gagacccgcc gtgcttttcc ttcatgacat aggtgcggcg aaaatcctcg
     3661 agcatttttt gggtcgccgt gtcccggctc ttttcggcca gggactcgac ctgcttgaga
     3721 atggcggcat cctccattcc ttcccggaac atctccagtc gaagggaagg atagacgttc
     3781 tgcccctggt gctccgggta gaggaacgtg ccgcgataca tcctcgatga ttgcttccac
     3841 gggtcttcat cccagtagtt catggaaaag aacaggtatc cgtccaaccg gtaccgccag
     3901 atcagccatc cgataatgcg cattcccttg aggggctcgt cgattcccga agccttgttg
     3961 cagtagatcc aaacctggga gcctttcctt tgtgcccgac tcacctgctt ttcgtcaaac
     4021 cagcggggat tcacaatgta gacgtcggcg atatcgagca gtgcgtcgtc gggctgttcc
     4081 gtaagctcca ggctgaattc cggaaatacc ttccgggcgg ccttgtagag aaggaccacc
     4141 tccggatatt gggaaggctt gggctcatcc cagagccgca tgctgagctt tcccttccag
     4201 cgggggtttt ccgcgattgc ctgcatgttc ttgcgatgct gggcgaggga cgccatgatc
     4261 gcctcttcgc tcacgggtcg gtacacgtcc gccagggact ttcgcttggt gaagaactgc
     4321 ccggcgggga tccgcatctt ttcgaccccg aggtcgtcaa ggcaaaaacg aaccatctcc
     4381 aggtagcggg caaaggtgtc ccgtcccttg tccagggtga ggtccgtttt tccgctcccc
     4441 cccagccccg caaaggaccg tacggcatag tcccggacca ggagggcggt catgtttttg
     4501 aaaatctgct gtcgttcctc cgcggccacc tttcgcggat aggtgtcgaa gagcggccga
     4561 atggtcgcct gaacaaaagg aattgcggcc ggatccagga cgaagggcag aacctcgtag
     4621 ggaacgcgga attccttttc ctctttggcc ccccgtagcc tgagggccac ctcccccctc
     4681 gccggtgcgg cccccctcga ggcctcgatc ttcacgtaca gatggatcag tcccgacgag
     4741 gcacgaacct tggcgcccgg agccgccggg accaggggat cgccgaaccc gcccgtaccg
     4801 ggtaggttca aggtcctgac ctcgtaaacc ctcaggcgga ggccttggcc gaattcctgg
     4861 gagaacagca gggcgagctc ggggtccgac gtccgcacgg ccaggatcat gccttcggct
     4921 tccccctgca ccagcaccag gggtcgaggc ttggcctccg tcgaccatgg cggcgcggag
     4981 gactgaatcc ggggcttctc ttccagcggc cgggccacga tctcggcaag aacgctgcaa
     5041 gccattgcca ggctcagccc catggccgcc acccccacac ccaccaaacg acgcatcgag
     5101 gatctacacc ggagcatctg cagccaccag cctttccgcc cgggacgggg actcgatttc
     5161 ctgggacctc tcccggatga cttccctggt taccgcgaac cacagagcca ggaaaaccca
     5221 atagagcctg gttcgcatgc cgttgtgacc cacgttgtac agcataatgg caaagaggat
     5281 tcccaggtag tacaggtcct cgtaccggcg catgaatctc aggtagggaa cggcgacggc
     5341 actcaagaag aagaggtaga tggcgacgcc gatcatcccg tagcacaaca gcatgtccgc
     5401 aaaggcgttg tgcacctcga tcaccttggg attgctggag actccgcccg acccgaacat
     5461 ggcggcgtat tcgctggcaa gagactgaaa aaatcgggtg ccgacccgtt tggacatggc
     5521 ggcttccgtg tccgtcatca tcaacctctg aacgaccagg atgtccactt ggggcagatc
     5581 gtttcgaaaa atgaccccgg ccgggagcaa aaggacgatc aacagggttc cggcgatcac
     5641 cactttccgc ctcagcttcc agaagatgag gcaatcgagc accccgatac agatcaaccc
     5701 ggcccgggat gcggacagga acacgaaagc gtggctcatg acccacacgg ccacggccac
     5761 aaacccggtg ccgattcgct gccaacgggt gaactcgaac ctgcgcgagt aaagtcgaat
     5821 caggaagaaa atggcgagaa tcaagatggc gaagtaggcc agctggttgg ggttattgaa
     5881 ggatagggcc gtccgcatgc tgacgccccc taccccttcg gtatccgtcg tgtcgtagga
     5941 accggtgacc aggaagggag gaatgaggga gagaaacagc ccggcataaa tacacaggac
     6001 ccccttcttc ttgaggatga ggcgctggag caggaccaca aaggcggcga acatgaatac
     6061 gatgaatacc agctggatgg ccgacttcat gtaaaacagg tcgcttcgtc ccacaaagta
     6121 gacgatgttg atcaaaacgg tccaagagac gtaaaccgcc agcagcatga caatcagacg
     6181 gctgcggtcg tacagttccc tgttcaggac caaggaggcg agcaccatgc cgaagatgat
     6241 ggggatgtcc accagctgaa accctccgga cggtaggacg taaaccggaa cggtcaggaa
     6301 gaacagcagg atgatgccgt gaaggatctt ttcggagaag cccaatcgtt gctggtcaat
     6361 catggctagt gggatcccac ccttctgaag tatcgagaaa ttctcttcca cacgatatcg
     6421 tcttcctccg ccatgcattg gccggtgagg aagaaggcgg caaacagcac caggatgacg
     6481 gcggtcttga tgagaagcga gaccacgaac tgggcgggaa gatgcatctg gtagtacaag
     6541 aacaagacca ggaccgaaac ggcggcgggg accatgtact tgagtacctc ccgggtgaag
     6601 ggatgcattc tgtacaaaag gacgatctgg cccaggcgaa gaagattgat ggcaacgatg
     6661 gcggacgaac tggctatggc cgccccgatc gctccgtaac ggggaatgag aaggtagttc
     6721 agggagaggt tcaggcccgc catgcagagg gaattgaaca gctcgagctt ctgtcgccgg
     6781 gtcatggtca gggtgacgcc cactcctccc gtcatacagg aaaccaggtg gccgaaggcc
     6841 agaatggaca gcgccgtcgt ggcactggat tcggcgtagt ccttgccgaa gatcatcaga
     6901 atctcccggc cggaaaagag cagcaggatg aaaatgggca cggttgcgaa acagagccaa
     6961 cgggtggtgg tcttgaaaat ccccgccaga cgcgccatct cgctcctctg gtaaagatcg
     7021 gcggccacgg gggcatagat ggaattggtg gcgatgagaa acacgctcat caccgatgaa
     7081 atctgggctg ccgcccgata gatccccacg tccctcgcgg aactcaggta ccccagcatg
     7141 agaatgtcgg ccgaagagag aagaaagtgc agaaatccca caaagagtaa gggaacggag
     7201 taggaaacca gggacccgat ttcgtagatc gcgggcaggt gttttcgaaa taagtcgggg
     7261 aaaagcttcc tgcagtagta gaccccggtc agcagagcgc ccaggttgga cagaatgaac
     7321 gcatagacgg ctcccagcag gccgaagccg gtgagataga aggcaatgaa tagaaccagg
     7381 ttcaaggccg gctgcatgat ctcccgggaa atcatcgtgt acttggtggt gtgaaatccc
     7441 tgcaggagcg aagaaaccac catggtgccg gcggtgaagg gaacggcaaa ggcaaaagac
     7501 tggaggacag gggccagttc cggcttcccg aagacgctcc cggccatgaa gtcggaaaga
     7561 aaaaagaccg ggaaacccac aaaacagccg ccaaccaggg acagggtcgt cgccgagatc
     7621 aagacccctt tgagctttgg caggtcgtgg tccttgtgga tggagacgaa cctcatgcct
     7681 cccatgttga ggcccatccg ggcaaaaatc tcgcagatct tcactgcggc gagccccagg
     7741 ctgtagaggc cgaattcctc gactcccagg agccgggcaa tgaggatctg gctgaggaaa
     7801 aagatgctct tgccaaaagc cgtacccacc agggtggctc ccgtaccctt ggccacctta
     7861 tggatgtccc ggctcgcacc cttttccccc gccgactccg ggggggctgc atccgattga
     7921 gtgtgggtca tcgccctcga tcctgggaaa cgggattcct ccagacggaa aggtccttgc
     7981 gcaggaggcc ttccaatgcc gccatgtcgg cgtcgtacct ttccagcagc tcccttcgaa
     8041 cttccgggct catccggggg agctcgcctg cattcccgaa gataccgcgc agccccaggg
     8101 ccttggctgc gttgatcacc caatcgaggt cgcttcttcg aaggaattcc ccccagttgc
     8161 gaaccacgga cccgaccttc ccgaatcggg atcggtagga gggattgacc ttccgaccca
     8221 cgaggttcga atcgaagccc ccgggttgga ttcccaggaa atttgccagt tcacccagag
     8281 ccccggccgg gtctttcatg acctcctcga agatcagcac caggaagttt tccccgggga
     8341 acaggtccat atatcgtcgg agctgccggt aatagagccc tcttccaaaa acgtcctctt
     8401 ccgccgccag aaaatctcga aaattcctct cctccccctt gttttgaacg tggtgcgcat
     8461 actgggaata cgctcggtcg gccggattgc ggaggatagc aagaaaacga cagtcgggca
     8521 aatgctcttt gatcctccgg gggacccggg ggtcatagag gtacatgggg gtaatctcgc
     8581 ccatccagcg aaccccgggc gattggtcgg cacggggaaa acaggtctcg taccactcga
     8641 gcccccgttc gtaataccga tcgaaaaagt gaagctcttt ccggcggcgg ggaaggtaga
     8701 cctcggggtg agagcgcatc tgcgcatgaa gccaagtggt tccggccctt tgggccccga
     8761 tcccgaggaa atttggcaac gtcatagcga atctcccgaa tagggctttg caacgggtga
     8821 atagtagcac aaccgaccgt gagggtggaa gggttgtcaa cagcggagga ggagactgcc
     8881 gaaacgcgcc ccgcaattac cggcggatcc ccgggaaacc ggaaggtgct cagcggctgg
     8941 aaattgactg ccgagagcta aaatcggaag atttcatgac ccgaaggacc cgggaattga
     9001 tcggacaacg ggaccatatc ccgtccatcc ccgcccgtga acccctcttt gagcggaaat
     9061 cccgtctcgt tatagagctc gattccaaag taccggacaa tctcgaggac ggtacccacc
     9121 ttccgttcat cgagaactcc cttccagaaa agagaagccc cttccctgga gacggatttg
     9181 tccattccgt acaacccgtg gagggtcttg agggtcgtca tggaaggggt gaagatgtga
     9241 gccgggtgca ggtcgatttc gggcatctcc aggtaggcca tcaattcctt taccgcagga
     9301 aagggtcggg catagaagtc ttcgtagtag aacaaaggca ggttgagatc tttcagttcc
     9361 ggcagggtga ccgcatgggt gatggcccac atgacggcaa ttcgttcgag gtccgtcttg
     9421 gcctgctcca ccacgggcag gcaccgggca atcctgtggc tcttatagtg gtcgtggtac
     9481 acggtcttgg ccaggaacag ttcataggtg ccgggccaac cgaattccca gaagttttgc
     9541 cgtttgagaa tggaagaaac cacggcacag gggtggcgga tgatgtagac gatctttgca
     9601 tcgaaattgt cgtgaagcca ggaaagcatg aagttggccc gtatttcctt gaatcccagg
     9661 atcccgcagt tttcccagat gagctccagg aagttcgggc ttatttcttc cagccgaacg
     9721 gggacgtgat ttcgcatcaa ccacatcttc ctgtgcctct tgcccatcac acggttcaaa
     9781 tattccttca gggaattccc gttcttaccg acgggagcgt aagagacctc ccgggaagcc
     9841 tcggtgacgc tggggtggaa aggctcgaac agaaccaggg tgccgctcgc cttggagacg
     9901 atgtcggcga tccaggtcgt ccccgacctg ccgaacccca ggatgaaaac ctttttcctc
     9961 atgccttccc cccactcatg catcgctgcc tctccgcgcc gaaataaata gtgcgtggca
    10021 tcgattcctc ggctaagatt cgcttaatgt aaggcccttt ttactgatac ccgacgacaa
    10081 ctctctggaa atcttctatc taccgttgcc tgcaaatatc ttttcagata ctttgggcta
    10141 gactagccat tccaatcccg tccaaggatt caacggcaga cccgcttatc aactggaacg
    10201 tgcaatctta caagtatatc tctctccttg tcaacgtcaa agtacggaaa gcacccctct
    10261 gccaaccggg aaacggcatt ccgagcgccg ctcttcccgg cactccccgt cagcccgtgt
    10321 tgcccagagg acggcaccgg gggtccggtc cccgggcggg cgcatccttg ccctcaaatc
    10381 ccgggaggcg gtcctccccg gggagaattc taccgcccgg caggtccccc cgtcttccta
    10441 agaccgaccc tgcagcctcg acaatcccct cgacttgaac gcgtcttctg cttatactcc
    10501 atcggttgtg atttcaggtc ccaaccgggc aaggaaaggg tgatgctttt caactcctat
    10561 acctacctcg ttttctttct tcccataaca gccggtcttt actttctcct cagtaggcgg
    10621 gaacggcatg gggaaggaaa aatccttctc attcttgcat ccctgggatt ttacggatac
    10681 cacgaatttc gaacgctccc ggtgctcctg gcgtccgtcc tggtcaacta tttttcctgc
    10741 accctgttca acgcggttgc cccgaggcgt cgcaaacctc tcctggtcgc cctgctgttg
    10801 ttcaacctgg gattgctcgt ctactacaag tattcggggt tcctggtaag caacgccaac
    10861 agccttttcc ataccggctt ttccatgccg aaccttctcg ttcccctggg catcagttac
    10921 ctcaccttcc tccagatcgc ctattgcgtg gattcctacc ggggagtcgt ccggaacaac
    10981 tcctttctca actacacgct ctttgccgtc ttctttccca aggtgatctc cggacccatt
    11041 gcccatcaca acgagatcct gccccaactg gacaatcctt cgaccaaaac cttttcctgg
    11101 caaaacctgt ccacggggct ttttctcctc agcatcggcc tgttcaaaaa ggtagtggtg
    11161 gccgacaact tggccccata cgtaaatttc ggctttgacc aggcccagtc cctgaacttc
    11221 ttctccggtt gggccacctc cctgggatat accctgcagc tctattttga tttcagcggg
    11281 tacaccgaca tggcgctggg gtccgccctg tttttgaatc tgaagcttcc cctaaacttc
    11341 gcaagtccct acaagtccac ggatatccgc cagttctggc agacctggca catcaccctg
    11401 ggacggttcc tgagggacta cctctacatt cccctgggcg gcaatcgaag gggaaagctt
    11461 tgcacatacc gcaaccttct cctcacgttc cttgtttgcg gggtgtggca tggggcggga
    11521 tggctctttt ttctctgggg ctttctccac ggcatcgcca tcgtggttca ccgcgcatgg
    11581 aatcagacgg ggatccggat gcaccgggtc ccggcgtggt tcttaacctt caatttcgtc
    11641 aatatcgcct ggatcttctt tcgggccagg gaatgggaag atgcactgaa agtcctcaag
    11701 gggatggtga atgtccaatc catcggctgg gagggagtgc aacttcgtca attcttcgtg
    11761 tattacctga aagaccacca gaggcatgcc ctggagatcc tggcgggagt ccagggggga
    11821 gaagcgatga ttctcctggt gatgctttgt attacggcct ttgtgatagt ggcgggaaac
    11881 agcaaccggt acctggagaa tttcactccc cgcttccggc acgtttgttt cttcgtggcg
    11941 tcttttctgt tttttgtgat ctgcaacacg tacctggcgg tgagaagtga gttcatctac
    12001 ttccagttct agggacccgg catcccacag gcggttcgta ctcctgtccc tgtcctttct
    12061 tcttctggcg gtgggtctca atttccttct ttgcgaggtc tttgcccgca aggtgatctg
    12121 gccccggaag tgcttccaac tcctcgaatt gcagagggac ggcaggacct ttcgtacggt
    12181 gagcctgggg acgtcccaca cggcggtcat gaacttcgac tacccggggt tgttcaattt
    12241 cggcctctcc cggaccttcc ccagcatcat gtatttcaag atgaagtacc tggccaaggc
    12301 ggcgcccaac gtccggaccc ttctcctgga agcggacact catctcttct acggtctggc
    12361 tgcgcactgc gacaagtatc agcattccgt ttacatcgac gaggacgtgg acccctccct
    12421 gggcaaccat ccgcggggca gcaaggggga gttcctctcc ttgaaggaag agatcgcgcc
    12481 gacggtctac aaacagatgg ccatgagcct tcaccggttg ctgacttccg gaaagaattc
    12541 atcggagaag acggcccgtt cggcggaaac ggaggacgtg tccgaggacg ttcggtggga
    12601 tctcctgccg cgggaagtcc gtgaacagac cgccatcagc aggctgaaac atttcagcct
    12661 ttacgactac gaacccatga accccctgct ggtcgagtat tatgaaaaga ccattcgcat
    12721 ggcgctaaac caggggatga gggtcatctt catccggtac ccgctgacca acgagtttct
    12781 ggcccaccac aatccgcaga tcgaggacga agtgtcggct ttcctgggcg ctctctcgaa
    12841 aaaatacggc attcccctcc tggactatcg caaagtgttc gtggatcgac aggacctttt
    12901 cagcaaccag gaccattgca acggcgaggg gaaaaaggtc ctccgggagc gaatccttgc
    12961 cgaccttcga gggctggggg tctccatggt gagcgccgct gccggggaag gcagccccga
    13021 cggtccccaa aacccgtaag aattttcccc ggaaaaggtc ctcatccgaa gttgcattca
    13081 agctgaaact gcccatccgc aggactcagc cctttgctcg aaatcttcac tcagcatgtc
    13141 agggcgacct tgggcgcaac ctggtatcac gggtggaagc cggagggaga tatttcctcg
    13201 attgagcgac ttggccggtc ggaatcccgt ttggtcattt ttgcgtaagc gggaatccgg
    13261 aaagagagtg catacccgtt ggatgcccgc cttggcgggc atgacgcctt gatgctctcg
    13321 gaatcgctca acttaggttc ttatctgtgg atgggtcttt agcaggcttt ggtccgcggg
    13381 agatcgcccc tgagggcggg catctttatg atgcggttct cgatcagaaa acccactatc
    13441 tgttccgttg attcctcgag ggtctggacc ccggtttcca caacgaggtc cggagattcc
    13501 ggctcttcat aggtgtcccc gatccccgtc atgtcggtga tttctcccct ccgagctttg
    13561 gcatagagcc ccttggggtc ccttttctcg cacacttcca ggggacagcg cacgtagatc
    13621 tcaaagtacg gccatccctt catcaattcg cgaatgaacc ggcgactttc cccgtacggg
    13681 gtgatgaacg ccgaaaagac caggatgcct gcatccacca tcagcttggc cacctcggcc
    13741 acccgccgga tgttttcctt ccgaccctcc ggactcaggt tcaaatctct attgagaccg
    13801 gaacggatgt tgtcgccatc caggacatag cttcgaattc cgaacttgtg gaggcgggcc
    13861 tccaccgcat gggccagggt ggatttgccg cttccggaca gcccggtgaa ccacagaagt
    13921 gcactacggt gcccattgag ctgatggcgg tccgcacatt ggatcgtggg tttgaacggg
    13981 taaatattca tcaattcaga agcctcatca agtctgggat gtgggtccct tcaacggtcc
    14041 cttccctccc gtcgggtgga acgtctcggg gaatccgtgc cgtgtttcct gctccttaga
    14101 tcccccctct tccccgcccc cgaaaaaccc cgcgagactt tgcgccaaag ggcggcaccg
    14161 ggaaaatccg attctcggtc ccgggaaagc cgggtcaaga agcctacttg taggatagag
    14221 atgcgttctc gcttgtcaac aagaatatga gaaggatgca aggtgcggaa tgcttgccgc
    14281 atatggcgaa aaccccggtc cctctcgcag ttgacttcaa gtatcccata tgctaagaga
    14341 cggcggaatt tatgattcag ggggaaactg cggttgggcg ctcatgcaaa agaggcagtc
    14401 ggtctggaac aagagttcat tatctcgaac aaattgggaa tacacgcgcg cgttgcagca
    14461 cagatcgtga aagtggccgc tcaatttcaa tccgagatat ggatcatgaa gagcggaaac
    14521 agagtcaacg gcaaaagcat tctggatgtg atgaccttgg tgtgtcctca cggcagcagg
    14581 attcgcgtct ccgtcgacgg accggacgcg gcggccgcca tggatgcgct ggcccacctg
    14641 tttcagacaa agttcggtga gtcataaggt ttttcctcct tcctgagcac ttccgatggt
    14701 tttgcggtcc ctaccgagta gtcgttgtcg ggcatagtga aacggaaagc ggctcatgcg
    14761 tcccatcccg gcgtcgagtc ttcccaatcc ccgggcagga gtcctccaaa tcctgatcag
    14821 aaacgaccca atcaaccgtc gcgttcggtc cgggcccaac gcccttatcc ggtttgcagc
    14881 gtacctcgcg ggtaattttc atgaacccat cttccgaagt cattgaagga atcggcgtct
    14941 ccccgggcat tgccattgga aaagcctacc tcgtggagca gggacgcatt cccattcccc
    15001 atcacaccat cgaagatgaa tccggtgtcg accgggaatg ccaaaagttc caggacgccg
    15061 tcaacaaggc ggaagaggac ttggaggaga tcaaggcgcg gatcctcccc gtgattaagg
    15121 aacacgcccg ggtcctcgaa gtccaccaga tgatcctgcg agaccccctg atcttcggag
    15181 aaacccttcg actgattcga caagagcggc tcaatgccca atgggccctg caacgttccc
    15241 ttctcaaggc ctacgagctt ttcgaatccg tcgaggacga gtacgtgaga agccgcacca
    15301 ccgacatcca ggcggtggga gaacggattc ttcgcaacct ggcgggcaag gaggaaaacc
    15361 tcttcaacag catccgcgaa cgggtgatcc tcgttgccca cgacctctcc cccgcggaca
    15421 cagcccagct gctcctggaa aagaccctgg gattcgttac cgatatggga ggacgcacct
    15481 cccatacctc catcctggca cgatccctga acattcccgc ggtggtgggg accgaacggg
    15541 ccacccaggc cattccgtct ggatgcctcg tgatcctgga cggctccgcg ggaaccgtca
    15601 tcatcgatcc ctccgacgag gaaatcggct cctattacga acgccagatc caactggagg
    15661 agtaccagaa gagcatcacc cgtaccgctc acctccccgc caggacccgg gacgggcacc
    15721 tggttcgagt ggaggccaac atcgagctcc tggaagaagt ggttgccgtc aaagacaacg
    15781 gagcggaagg gatcggtctc tatcgcacgg agtttttcct gatcaaccga aagaacttcc
    15841 cggacgaaaa cactctctac agcaactacc gggaactggc cgagctgatg gctccccact
    15901 gggtcaccat gaggaccctg gattttggag cggacaagct gggggcctgg taccccaaaa
    15961 tggaggaaac caaccccgcc ctggggctcc ggtccattcg cctgtgtctt cattaccggg
    16021 atctcttcaa gacccagctg cgggcgatcc ttcgagcgag cgcctcgacg aaaaacgttc
    16081 gtttgatgtt tcccctcata tccggggtgg ccgagctcct tgaagcccgg ggggtattgc
    16141 gggaggtccg ggaggagctt cgcagggaac gcattgccta cgacgaagac atgccggtgg
    16201 gggtcatgat cgaggtcccg tctgcggtgg ccgtttcgga tttgctggcc cgggaggtgg
    16261 atttcttcag catcgggacc aacgacctga ttcagtacag catcggaatc gaccgggcca
    16321 acgagcacgt ggcctacctg tacgagcccc tccaccccgg agtgttgagg ctgatcaaga
    16381 tggccgtgga agcgggccac agggcgggaa ttcccgtgag tctctgcggc gaaatggcgg
    16441 gggaatcctt ctatgtcccc attctcctgg ggcttcagct ggattgtctc agcatgaatc
    16501 cccagtcggt gccccgggtg aagaacctga tttcccgttc cctcctgaag gactgccggc
    16561 ggttcgtgaa aaggatcttg gaatccagca cggccaaaga aatcaatgaa ttgctccagg
    16621 aaatgatgct caagaatttc cccgaggaat accggctctt cgaccctcga gccctttcgc
    16681 cgaggatcct gtccaaacgg cgccgagttc cccaacggtt gtaggtcgcc cgggggttgt
    16741 ttccgcccgc aagaacaaag ggttctctga tggccaaaga agcgaaaaac agtgaacgga
    16801 gcccgatccg cctcgtatgc cggaacaaga aggcgtattt cgagtacgaa atcctcgaga
    16861 ccttcgaagc gggcatgatg ctgctgggta ccgaggtgaa gtcgctgcgc gagggccggg
    16921 caaacctgaa ggacagctat gcccgcatca agaaggggga ggtcttcctt tacgatctgc
    16981 acatctctcc ctatcctcat gcgtcctaca acaatcacga accggaacgg gtacgcaagc
    17041 tcctgttgca cggcttcgag atcaagaggc tccagggaaa gacccaggag cgcggcttgt
    17101 cgctcatccc cctcaagatc tacttcagcc acggcagggc caaggcggaa ctggccctgg
    17161 cccgaggaaa gaagctccac gacaagcgag aaagtctgaa gaaaaaggat gaagctcgag
    17221 aactgcaacg cttgcgacgc cgagacaaga tttcctgacc ccacgaccac ggacggaaat
    17281 cgggcatgaa aaggacttcg gtcccaaggt ccaaggtcgc ccccatgaga tgcctgacgc
    17341 tcacggctcc ccactccctg gttcttacgg accgtccccg gccacagcct gcagaggacg
    17401 aggttctgct gcaggtgtcc cattgcgccc tgtgcagaac cgacgccaag atgtggcagc
    17461 agggccaccg cgatctgctc ctcccccggg ttctcggcca tgaattctgc ggatctgcgg
    17521 tgggcaccgg tcggcgtttc gtggtttggc cgggtcgggc gtgcgggcag tgcgatgatt
    17581 gcctgcaagg cagggaaaac ctttgcccgt ccatgcgaat cctaggattt caccgagacg
    17641 gcggccttgc tgaattcgtg acggtacctg catcggccct catccccatt tccgacgacc
    17701 tcgatcccgc cgtcgcctgc cttgcggagc ccctggcctg ctcattgaac ggattggccc
    17761 gggtccgggt ccaagaaggc gagcgggtcc tcattgtcgg aggagggacc ctgggaatcc
    17821 tgctggccct gggagccgtg agccggggag cccgggtctg cgtggtggat cgaaatcctt
    17881 ccaagctcca tcgaagccg
//
LOCUS       DAOOVC010000073        17897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2159_length_17897_cov_5.661305, whole genome shotgun sequence.
ACCESSION   DAOOVC010000073 DAOOVC010000000
VERSION     DAOOVC010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2159_length_17897_cov_5.661305"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2128..4086
                     /locus_tag="PLM79_10465"
     CDS             2128..4086
                     /locus_tag="PLM79_10465"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOV86776.1"
                     /translation="MNRDSRVVCQVLSCCTALLIFAAPLFGQGQLIPTSNPHWSWQNP
                     RQHGEHLGAASFISTREGWAVGIMQGVLHTTDGASSWTAQRFGPPLGFNDVAFRDRNG
                     VAVGNGSNKWGWTEESIIWFTRNGGETWKQSYMDKKGGGFGGVAFSTPLKAWAVGRHG
                     TILRSSDGGKTWDKVLLPKIAKDYDFTDVAFADTMHGLVVGSSNSARNLFMLTEDGGA
                     HWTPVRFRSKGALWHVAFPTPKEAWAVGDFGYIYHSSDGGMTWARQKSPHTSPFIYAT
                     SVLFHNSRNGFVTTSDGFVLATSDGGKNWKVSARRVQGINALASFQGKNRFWAFGADG
                     TALRTDDFGATWKVLSKSIREHLLAVSFVSPNFGWAVGTNATILRTGNGGLSWIQCKS
                     PVPSNITLRGVFFVHASRGWVVGDRGKILTTDDGGQTWSVQESGTAENLGAVYFIDTQ
                     RGWVGGANRTLLRTEDGGANWKPVSITADIDIYAIKFSDPHHGWAVGESKDRLLRTED
                     GGDTWTEVEVTFSWLQEAMGLYSLSFINAYEGWTCGAIQVGTDLATLIAHTADGGLTW
                     QEQPLSSGTSVDILRAMVVKPDGRGWAAGEHGTYWTTNDAGNLWTMRVRPTPDALIMG
                     MAFPTNDIGFMVGEGGTILKTNDAGGLD"
     gene            5358..5618
                     /locus_tag="PLM79_10470"
     CDS             5358..5618
                     /locus_tag="PLM79_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86777.1"
                     /translation="MMKVERLYHKCGHAILYAKETVFPATKTFFIDGENPFALEKDGN
                     KKPRVVDRCPNCGGSIRIEKLFSQKPEVREEKRTSGYIPAGR"
     gene            complement(5740..6654)
                     /locus_tag="PLM79_10475"
     CDS             complement(5740..6654)
                     /locus_tag="PLM79_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HOV86778.1"
                     /translation="MSDTLSRDTVTEKISVVEGMIPVQGGRVWHRTVGADRKKVPLLV
                     LHGGPGAPHDYLEPLEHLGKDRPVVFYDQLGCGNSDRPDDPTLWTLDRFVDELARVRE
                     ALDLQRLHILGQSWGTMLAVEYVLTRKPEGVLSLILSGPCLSVSRFIEDQKAYLLDFP
                     QDVQETIRRSEESGDFRSREYQDAMIMYFSRHLCRLQPWPNCLIETLEKMGLNVYETM
                     WGPSEFTLTGTLKDFERAERLKEIMLPTLFTCGRHDEATPDTTRFYQEMLPGSRMAVL
                     DDASHEHHLEQPVRYLETVRQFLGEIEE"
     gene            7134..7949
                     /gene="budA"
                     /locus_tag="PLM79_10480"
     CDS             7134..7949
                     /gene="budA"
                     /locus_tag="PLM79_10480"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HOV86779.1"
                     /translation="MKDPLQSKDTVKITVDLPRDLVAGLDAVRESTGRGLPELMEQAL
                     GQFLRGEERTEVLYLSAPVDALMKGFYEENTRLAELRSHGDFGLGTFNHLDGEMVMLD
                     GVVYQLKADGTALIVRDEVETPFACVTAFHPTTVEEFEGELEYAGFKNLLDRLIPSEN
                     LFYAVRVDGLFSNVKVWSVSKQSPSQPISSVTPSLREFGDIEGTLSGFYTPRFIKSIS
                     MAGYHLHFLTADRTRGGHMYQCSLKKARISLQFLRKLELNLPVTIDYLTAHLI"
     gene            complement(8461..8637)
                     /locus_tag="PLM79_10485"
     CDS             complement(8461..8637)
                     /locus_tag="PLM79_10485"
                     /inference="COORDINATES: protein motif:HMM:NF018715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOV86780.1"
                     /translation="MMLVSLPIMFLLIAVMSGILGFTGLIGGASWVAQISFLVFLVAF
                     LYASIRGRGKAPPP"
     gene            complement(8771..9439)
                     /locus_tag="PLM79_10490"
     CDS             complement(8771..9439)
                     /locus_tag="PLM79_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86781.1"
                     /translation="MKEIFALVFFGILAGATIILAVRRRIGPTLTITLLIFSLSSGFL
                     IANYDWLQRMHGHCPDGEYDRMQLTALRNQAVRDFAQIARREAEDLASRGDRIAQDLE
                     NWESRLKAAESVVQSMKNLEEEIRRQERSLKESNERLEKGRQQIMAAYQDMAELALMT
                     VRLAWLQIQTKDDSNSVRARTATQQMLDLLDQIVVLVIADPEARARFYDEVMTSLPPK
                     EQNP"
     gene            10006..10458
                     /locus_tag="PLM79_10495"
     CDS             10006..10458
                     /locus_tag="PLM79_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HOV86782.1"
                     /translation="MLSILLCAGGGTALGGRLTAQDLKSCPSCFFCGMDRKCFAYSRV
                     VIRYDDRSTVGACSINCAALDLAKSIDRTPVKLLVGDYKKRSLIDAERAVWIIGGDKP
                     GVMSERAKWAFASRRDAEEYIRKHGGALAGFDEVMIATYLDIYRETVK"
     gene            10572..10967
                     /locus_tag="PLM79_10500"
     CDS             10572..10967
                     /locus_tag="PLM79_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86783.1"
                     /translation="MKRAFLVPLILAFALTLVSVSFAEESGRLDLKVGDEVYVCGCGK
                     GCPCDTIAMKPGKCVCKRKLVKAKVVKVEEGKATMLVGKKETVFKTIGKYACACGPGC
                     DCGTISQLPGKCACGKAMKKVSDVKKKST"
     assembly_gap    11452..11551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11552..13724)
                     /locus_tag="PLM79_10505"
     CDS             complement(<11552..13724)
                     /locus_tag="PLM79_10505"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HOV86784.1"
                     /translation="MIRLARFSILALAVLLSTAPCVLAQAQGGDDGGVLVGRISHVEG
                     QVLRYVPEDDDWVITVKDAPFGFEDALYSDKDSRVELLLPNSTQVRMGEDTQIQLISL
                     QDDLTEMDVASGVARFYNKSKDGVVRVTTPYGYVLAGAATVFDLYVGDESLEIIAIRG
                     TVDFVTEKDQSKYEVIAGSESLVSDGSQVTADDGTVDVAWDDWNTERDNLWSRRLQVK
                     GDSVRYIPESLHDQAYELEENGRWDRVYYNGEHRMMWRPTHVDSDWAPYSEGRWTEWY
                     DDNCWIPEEPFGYTTHHYGSWAYIDDCSCWYWAPPVVRVHVGPSYGCCCCWNPGRVAW
                     IHSEADIGWVPLAPWEPYYCHRPWFPGAVVVGGVGVAALGVGLAIGSLAYVGHAVLVP
                     QVNFWGVHRYSSVRIRNINKTVIINNYKTTKVINKTVINNYDSRRARRNFTNAVYKGK
                     PRANLNRINQNRKLAAQERKINARDYERRVKNLKEGRPSGDARLQKPKVANRDLKKQG
                     RQVRDRDVRRPGDRREDRKGDRDRIRPPKRDQIEREKARRDDRGRMDRDERAKAREER
                     RIEREKARRDDRGRMDRDERAKAREERRLEREKARRDDRGRTREERRQQMDQDRRQKR
                     EVQQQQQRERRQQMQEERRQRRQERQPQGQDVRQQRQQQRQQQLQQRQQQREQRPQQM
                     QQRQERQQQRQQQQQQRQQQMQQRQQQQQRQQQMQQRQERQQ"
     gene            14105..14629
                     /locus_tag="PLM79_10510"
     CDS             14105..14629
                     /locus_tag="PLM79_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV86785.1"
                     /translation="MQRQETPERLTVKAAARTLNVSTKTIHRYLAKGFLTKIKEGTRT
                     LVSLQELTAYLGRVSQGQGPGLEGTQPRTQKGQSGDTVTLDRERYESLLMEIGELRKG
                     SRLMGELKQLVEGRDQGSGPDRKELEELAARVQYLELRGLEREKQWVELLSRVKDLEA
                     DLSRMKMQKPWWQK"
     gene            complement(14804..15997)
                     /locus_tag="PLM79_10515"
     CDS             complement(14804..15997)
                     /locus_tag="PLM79_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOV86786.1"
                     /translation="MQSFEVLKDLYWVGAVDWNIRDFHGYSVPKGTTYNAFLLMDEKV
                     ALFDTVKKPFKDTLIHNIRKVVEPQRIDYLIVNHVEMDHSGCVPEMVDLIRPEKLICS
                     PMGKKALIQHYHREDWPYEVVETGRNIPLGRRSVQFIETRMLHWPDSMLSYVPEARLL
                     ISSDGFGQHWATSERFDDQVDLPELFHHAARYYANILLPYSPLVQKVLAKVKELGLAI
                     DMIAPDHGLIWRSNVSKILDSYATWSLQKPKSKALLIYDTMWQSTEIMARSIAKGLME
                     EGVSVRILNLKTNHRSDIITEVLDAGALILGSPTLNNGILPTMADLLCYMKGLRPVNK
                     IAAAFGSYGWSGEAVKVINDALKEMKFDVLEPGIRVHYAPTHDDLQRCLEFGRKIGKA
                     VKERG"
     gene            complement(16043..16237)
                     /locus_tag="PLM79_10520"
     CDS             complement(16043..16237)
                     /locus_tag="PLM79_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOV86787.1"
                     /translation="MGGSDCLYQCQTTNCGYLYDPKRGDRKGKIEKGTSFDDLPEDWK
                     CPVCGAGKKMFKPVPGTGCD"
     gene            complement(16285..16860)
                     /locus_tag="PLM79_10525"
     CDS             complement(16285..16860)
                     /locus_tag="PLM79_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV86788.1"
                     /translation="MPKLVGTRTEKNLLTAFSGESQARNRYTYFSSQAKKDGFVQIAA
                     IFEETANQEKEHAKRLFKFLEGGEVSVTATYPAGRIGSTLENLREAAAGENHEHTVMY
                     PEFAAVAREEGLPEIAEVFEAIAVAEKQHEKRYKALAGNISANRVFQRETAVIWRCIN
                     CGYLHEGAEAPELCPACAHPRAHYEILGENW"
     gene            17090..17311
                     /locus_tag="PLM79_10530"
     CDS             17090..17311
                     /locus_tag="PLM79_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86789.1"
                     /translation="MQEEENRLRRLRRLVDFSLALIAQSDLALEEAQNLHRMVRNTAS
                     RLFPGKEAAFDLIYTPRFRRLIAEKYALQ"
     gene            17433..>17897
                     /locus_tag="PLM79_10535"
     CDS             17433..>17897
                     /locus_tag="PLM79_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86790.1"
                     /translation="MDLWILKLQREATRIASRHRIPEFYVRFKAPMAVARKLFFTNSS
                     VVYLRGMVRPILQDEMGHGLLHSTRVGLDAATLIDVELMSSPMSREEVERLMVLGLVA
                     GLLHDICRNESNHAESGALEAARVLQNLPMTREESRCICLAIRNHEAFVAPVL"
BASE COUNT         3638 a         5166 c         5119 g         3874 t
ORIGIN      
        1 gtttgcgcca cggaatggtt cctccgggag acttccgcct cagttctccg ctctaaaacc
       61 gcaacaggtc gttccactgc atgggaataa acgtcagggt gccccgccct cagagatgat
      121 tgtaattatc accgcaatcg atcactgtaa agcagagtcg aacggaggtg ccgtggcggc
      181 aggccccgag tgggagaccc ccgcatcttt tcaggtgagg tctcggtctc ggtaaccgta
      241 cgtacccggt ttcctccgag cgctcatcga accggacgtg cgggtttccc gcatccggct
      301 ctcgggcggg gctcacatca acccattcgc ggagcccgaa ggggcgcctt ttgaacagag
      361 gaccccccaa gacttccaaa gacctttttc atcgcaaata cggcggtacc acggccctcc
      421 attttaaagc ctcgccgaag gaaacgtcgg acttgatcat acacggaacg atcgagggta
      481 tgccgcacct tgctgtggct cccgtagcag aagtattcgt ccagccccga agcgttcggt
      541 tgagagcggt gcacacctca tccccgccgt gttctgaggt cgaagaatcg agcgaatccg
      601 tcctttgatc ctttgcaggg ccttctgcga agacttggct cccatgtacg gatcccggta
      661 cgccgaaaga ggaaagggcc aaaggtgtag gccgggaaat caaacgtctc ccggtgtgca
      721 ttgcggatgc aggtcttttg ttgagcttga gcttgagact gtttagcgaa caatgtggaa
      781 cttttggatc cacgaaaaac cggccacgct tgccaccgcc tgcgaatcgt tttcgaggag
      841 accgagagct ctgaccagag ggtcctcaac agcacccacc catgctttga aagaccttgt
      901 ttcgaaacca attctctcgg atgctccgcc ggaagtgttc aaccgggttg acctcaggac
      961 tatatgatgg aaggaacacc aggtgcatat tccctgggac tttcagctct cgggctctgt
     1021 gccaacccgc tccgtcaaga accattagga cgaactcgtc cgggtgtctc ctgcccacct
     1081 cttcaaaaaa caaagacacg gctccctcgt ttgcagccct cctgggcttg ttggtcgctc
     1141 tttggagacg cggcctgggc aggacccctc gccggccgtg ccggtcaagc atgcggtaga
     1201 tggtggtctt ggcaaccttg cggcccacga gagattcaaa tgcctgctgg atcttcacga
     1261 cggaaatcac tcaacccttt tgggcttcac cttgaaaatt ggaaaggaac cggcgctctt
     1321 cctgaaaact catggagcag cgccgccggc ctccccagtt ccggtcggca gggacggttc
     1381 cggagtgtcg ctccccaaat ggctttctca gaataaccgt tccccggcat ccacccggaa
     1441 gctgtgcgcc gattcggtca tctacaccaa ggagtacagg gagactgcct gagccgctct
     1501 gagttcttcg gccgtctcgg ccttttggaa tgattccgtc gctgactgga acatgtcgtc
     1561 cggggtcctg gtctttctcg gcaccggcaa ccccctccat ccggtgaggt ctacactata
     1621 tccgggatgg agcgattctt tcgcagccgg cggagatatt tacatccata caaatagcgg
     1681 cggttcagta tgggtcgccg gagctgatca agacgaagat ggtcccggaa cggtccggac
     1741 cctcgatggt tcctctaccg gcgcggcgca ccggcttcgc ggcaattgtg cggaattcgt
     1801 gttggtgccg cttcgtcaga caataccgga aagtcggctt attgggcggg gttctttttg
     1861 tctatgaacc ccgttcaact ttggctctcg gaaaagtcgc caagaacctg cctctttgga
     1921 aatagccgga gggttaatca atatgcacat agcgcttgac attgcccctc cgtcatttct
     1981 atttcctatt cgtcccgtta ggttgtgaaa aagtgattcc cccatcccgg atcatgttct
     2041 cgaggcggtc tattatgccg catcgggacc agggagggtc cgtcactgca aaaaaacgag
     2101 ccgccgatcc acgaagagga ggggaacatg aaccgtgata gccgggtcgt ctgccaagtc
     2161 ttaagctgct gcaccgctct tctgatcttc gccgcaccgc ttttcgggca aggtcagcta
     2221 attcccacca gcaatccgca ctggtcgtgg caaaaccccc gtcagcacgg agagcacctg
     2281 ggtgccgcct cttttatcag caccagggag gggtgggccg tgggaatcat gcagggagtg
     2341 ctgcacacaa cggacggcgc ctcttcttgg accgctcagc ggttcggacc gcccctcggg
     2401 ttcaacgatg tggcatttcg agaccgcaat ggcgttgccg tgggcaatgg cagcaacaag
     2461 tggggctgga ccgaagagtc tatcatctgg ttcacgagaa acggcgggga gacctggaaa
     2521 cagagctata tggacaagaa gggcggaggc ttcggcggtg tcgcgttttc cacacctctc
     2581 aaggcatggg ccgttggccg tcatgggacg atactccgta gctccgacgg gggcaagacc
     2641 tgggataagg tccttctccc gaagatcgcg aaggactacg atttcaccga cgtggccttc
     2701 gccgacacga tgcacggtct ggtggttgga agctcaaact cggcccgaaa cctcttcatg
     2761 ttgaccgagg atgggggagc ccactggact cccgttcgat tccgttcgaa gggcgctctc
     2821 tggcatgtcg cctttccaac cccgaaggag gcgtgggccg tgggtgattt cggatacatc
     2881 taccattcct cagacggagg gatgacctgg gcaagacaaa agagccccca cacaagcccc
     2941 tttatctatg ccaccagcgt cttgttccac aactccagga acgggttcgt cacgacctcg
     3001 gacggattcg tcctcgccac ctccgacggc gggaagaact ggaaagtgtc tgccaggcgg
     3061 gttcagggca tcaatgccct tgcctccttc cagggtaaga atcgtttctg ggccttcggg
     3121 gcggacggca ctgccctccg gaccgacgac ttcggcgcca cctggaaagt cctctcgaag
     3181 agcatcaggg agcacctctt ggccgtctcc ttcgtctccc ccaacttcgg ttgggccgta
     3241 gggaccaacg cgactatcct gcgcaccggc aatggcggct tgagctggat tcagtgcaaa
     3301 agtccggttc catccaacat taccctccgg ggtgtcttct ttgtgcacgc ctcgcgcgga
     3361 tgggtcgttg gagatcgggg caagatcctc accacggatg atggcgggca aacgtggtct
     3421 gtacaagaga gcggcacagc cgaaaacctg ggagccgtct acttcataga cactcagaga
     3481 ggctgggtcg gtggggcgaa ccgtaccctt ctccgcaccg aggacggggg ggccaattgg
     3541 aaacccgtga gtatcaccgc ggatatagac atttacgcca tcaagttttc cgacccccac
     3601 catgggtggg ccgttggcga aagcaaggac cggcttttga gaaccgaaga tggaggagac
     3661 acctggaccg aggtggaagt aaccttttcc tggttacagg aggctatggg gctctactca
     3721 ctatccttca tcaacgccta cgagggatgg acctgcggcg ccatccaagt tggtactgat
     3781 cttgcgaccc tcatcgccca cacggcggat ggaggcttga cttggcagga acagcccttg
     3841 tcgtccggca cttccgtgga catcctgcgg gcgatggtgg tcaaacccga cgggcgtggt
     3901 tgggctgccg gagaacacgg gacctactgg acgaccaacg acgccggcaa cctctggacc
     3961 atgcgggtcc gcccgacccc cgacgcgttg atcatgggca tggctttccc caccaacgac
     4021 ataggtttca tggtgggaga aggcgggacg atcctcaaga ccaacgatgc cggaggactt
     4081 gactagtttt catccctaaa gggccaacat gctgaattct ttgcattgat ggcatctttt
     4141 gccggcgaac agcccgagtt tgcgacatag tgctctctca taatcagccg ttttcctgaa
     4201 acaaaggcca tgggagaggc acaaaatgcg gtacaaaaga gtgggccggc tcagctttcc
     4261 catatggcgg gagagggtga gatcagcaaa gggccggagg cggtctcgac ggtcctggat
     4321 gccgacccgc agatctttga gcattccctg aaggatcttg tgggcatcaa ggatgctcag
     4381 acgggcagat tggggatggg tacggtgccg agcgggggtt cgctgtctct atctcaagca
     4441 gtcccgaagt ccgacttaca aggagcttgc cttccattag gagggatcga aagcttttcg
     4501 ctgctttgcc cgcccggatc acacccagta cccgagcgat tcgactcttc aggagaacat
     4561 caaagccatt ggcctccaga ccctgggagt tcataaaccg cgagctcata aggtatggcg
     4621 aagaggaggg gatcgagcgg gggcgtaaga tccggattga ctccacagga gttgagtcca
     4681 acatccatca ccccgccatc ctcccctctg tgccgggtgc atatgttcga gtaacttgcc
     4741 accttcaggg gcccaatggt ttacaatcgc tctgagcggt ccacattttc aggcctttgg
     4801 gtttgccggg gtccctgaat tcttgaacca ggtgtctcaa agtggttgac acattccggg
     4861 cactcggact gaaacttctt ttccgggcgt ggatgtcaat cgattgcaac cgcaatggga
     4921 ctggaggaaa gctcaaggga agctctcagc cagatacgct gaaccctctg aagtttagat
     4981 ttgctccgga cttccgtcag tcttttgcgc aatgactcgg cggccccttt cggcaagggc
     5041 tttggtatcc tcgtatttca ctcctcctga ctgaatacct tgatttcatg gaggatatat
     5101 tgggtatccg gtgaatgcaa cctgggatca tatcccgaaa aattcgccag tttgagccga
     5161 tgccgtcacg aagatcgagg gcagcgaata ccaaatccgg gaggttgtgg taggaaacga
     5221 agcagaaagg cttgttcctt ggaacttcgg gttaatgttc ggagctccat gatccgaatt
     5281 aaacggtgaa accttgcgcc acggaagaaa taaccgggct tttcgaagcc ctttttcgaa
     5341 cccgaaagaa agtcacaatg atgaaagttg aaaggctgta ccataagtgc ggacatgcca
     5401 tcctctatgc caaagaaacc gtctttccgg cgacgaagac ctttttcatc gacggggaga
     5461 atccgttcgc cttggagaag gatgggaata aaaagcccag ggtggtggac cgatgtccca
     5521 actgtggagg ttcgattcga atcgaaaagc tgttttccca aaagccggaa gtccgggagg
     5581 aaaagaggac ctccggctac atccccgcgg gaagatgact ctttcgcccc cctcgtcttc
     5641 gggctgcccg gcgggataag cccgtcgtgg aagaccgcgg catcccaaga gggcggaaac
     5701 gagaggattg agggtcttcg agaagagcag gcccttgggc tactcttcga tttcccccag
     5761 gaactgccgc acggtttcca ggtagcgcac gggttgttcc aggtgatgct catgggaggc
     5821 gtcgtccaac acggccatcc tggatcccgg aagcatctcc tggtagaatc gggtggtgtc
     5881 gggagtcgct tcatcgtgtc ggccgcacgt gaacaaggtg ggaagcatga tctccttgag
     5941 acgctcggcc cgctcgaaat ccttcagagt cccggtcaga gtgaattcgc ttggacccca
     6001 catggtctcg tagacattca gacccatttt ttcgagagtc tcgatcaggc agttgggcca
     6061 cggttggagc cgacacaggt gacgggaaaa gtacatgatc atggcgtctt ggtattcccg
     6121 ggaacggaaa tccccggatt cttcgcttcg ccggatggtc tcctggacgt cttggggaaa
     6181 gtcgaggagg taggcctttt ggtcttcgat gaagcgggag acgctgaggc acggacccga
     6241 gaggatcagg ctgaggactc cttccggttt tcgggtcagg acatattcca cggccagcat
     6301 ggttccccac gactggccca ggatgtggag ccgctggagg tcaagggcct cccgaacccg
     6361 ggccaattca tcgacgaaac gatccagcgt ccataaggtc gggtcatcgg gccgatccga
     6421 gttgccgcac ccgagctggt cataaaacac caccggccgg tccttgccca gatgctccag
     6481 gggttcgaga tagtcatgag gagctcccgg cccgccatga aggaccaaga ggggaacctt
     6541 cttgcggtcg gcccccacgg tcctgtgcca cacgcgcccg ccctggacgg gaatcattcc
     6601 ttccaccacc gaaatctttt ccgtaacagt gtcccgggac aaagtgtccg acatgatttt
     6661 cctccacgac ttgagcggat cttgaaggcg aagacggggg tctcaaccag cttttcgtcg
     6721 gtcggaccgt tgccgtgggc tgcggtccgg caaattcccc acgacgattc ggtaccccgg
     6781 caggttctgt aacatacaaa cggtcccgat accacaataa ccggcctcct gtgcctcgta
     6841 caaactccac cccggcgggg cggcgcgaat tgcgcccccc tgttcatagt cggggtctgc
     6901 aaacaagcgt cggaggaagg aaggttgtgg gtgaaatgcc gggttgcctc cgcggaagtt
     6961 ccatttccgg ggattgcctg ctgtgttaga atgcgctcct atcccgacgg gccggcctcg
     7021 tctcgatgtt ctcccgggca ccgccgaggg ttatgggaga tcaatgatgc cttgttcctt
     7081 tgcctcgagc cgcagacccc gtgggcggcc atcccgtgtg aaaggagctc caaatgaaag
     7141 accctcttca gtccaaggat accgtgaaaa tcaccgtgga tctacctcga gacctggttg
     7201 cgggcctcga tgcggtccgt gaaagcacag gccggggact ccccgaactc atggaacagg
     7261 ccctggggca atttctccgt ggggaagaaa ggaccgaggt cctctacctt tccgcaccgg
     7321 tggacgccct gatgaaggga ttctacgagg aaaacacccg cttggcggaa ctgcgatccc
     7381 acggggattt cggcctgggg accttcaatc acctggatgg ggaaatggtg atgctggatg
     7441 gggtggtcta ccagttgaag gccgacggga ccgccctgat cgtgagggat gaagtggaga
     7501 ccccattcgc ctgcgttacc gcctttcacc ccaccaccgt cgaagaattc gagggtgaac
     7561 tggaatatgc aggcttcaag aacctattgg accggctcat cccatccgag aacctattct
     7621 acgccgtgcg ggtggatggg ctcttctcca acgtaaaggt ctggtccgta tccaaacagt
     7681 ccccctccca gccaatctcc tccgtgactc cttccctgcg cgagtttggg gacatcgagg
     7741 ggaccctctc cggtttctac actccgcgct tcatcaagtc catcagcatg gccgggtatc
     7801 atcttcattt cctgacggcc gaccggaccc ggggcgggca tatgtaccaa tgtagtctca
     7861 agaaggcccg catcagcctc cagttcctgc gaaaactgga actgaatctc cctgtcacca
     7921 tcgactactt gacggcgcac ttgatttagc gcaaagagta cagggccgcg tctgaggagg
     7981 cccgggattt ccccggtagc ccggtccccc aggggcccat gaacccatag gccggatttt
     8041 tccttttgcg gatcaaggag gtagtagaaa tgggcgtctt ccaatgcagc caatgcggct
     8101 tcgagaagga ggggcgatgc aagccccaga aatgcccaca atgtggaggg gcaaagacct
     8161 ttgaaaaatc ggagcaaaag gggcaagggg aaacgaaaga gaagtgagcc gaatccctga
     8221 tggcggactc ctctctcatt cccatccccc gacccggcct tgttgacgga gtttcctgcc
     8281 ctccggctgg gaggcaggga gcgcacccgc cgggatatcg aaggcggcgg ggattcctga
     8341 gggaccctcc ctcgatcctc gactccctgc gccttcgagg acgggacgaa gtccaattgc
     8401 agcctcaccg ggtccccccc ggccgtttcc cggggcggaa ccgggcccgc tcttgcgcac
     8461 ttatggcggg ggcgcttttc ccctgccacg aatggacgca tagagaaatg cgaccagaaa
     8521 gaccaggaat gaaatctgag ccacccacga agctcccccg ataagtcccg taaagccgag
     8581 aatccccgac atcaccgcaa tcagcaggaa catgatcggc aaggacacca gcatcatgac
     8641 cgatccttcc ccgcccgaag aaccagggcg gcaaatcgtt ttcgcaaagg aaaaacgggt
     8701 cttttctcac ccggaaattc cccgcctttg cccgtatcca atcgttcgaa cggcttccac
     8761 cagaatcaag ttaaggattc tgctctttgg ggggcaagga ggtcatgact tcgtcataga
     8821 acctggctct ggcctcaggg tccgcaatga ccaacacgac gatctgatcc agaagatcca
     8881 gcatttgttg ggtggcggtc cgggcacgaa cgctgttaga gtcgtccttg gtctggatct
     8941 gcagccaggc gagtcttacc gtcatcaggg ccagttccgc catgtcctgg taggccgcca
     9001 tgatttgctg acgccccttt tcgagtcgtt cgttgctttc cttcaggctg cgctcttgac
     9061 ggcggatttc ctcctccagg ttcttcatgg actgaaccac cgattccgcc gctttcaacc
     9121 ggctttccca gttttccaga tcttgagcga ttcgatcgcc cctgcttgcg agatcttccg
     9181 cctcgcgccg ggcgatctga gcgaagtccc tcacagcttg gttcctaagg gccgtcagtt
     9241 gcatgcggtc gtattcgccg tcagggcaat gcccgtgcat tctttgaagc caatcgtagt
     9301 tggcaatgag gaatcccgac gacagggaaa agataagcag ggtgatggtc aaggtcggac
     9361 caatgcgtcg gcggactgcc aggatgatgg tcgcacccgc cagaatgccg aaaaaaacga
     9421 gggcgaatat ctccttcatg cttcctcccc ccgttgccgc tttcctccgg acggccaatc
     9481 agccgcgaca agtaacacgc ctccaccttt cacccggtgc gacctccaag cccggcgaga
     9541 gaccaagcag ccggtccctc gaatcgatgg ctgatcctag aaagattcct ccgagacagg
     9601 acctacatcc cgggtcgaaa ggacggatag tagtcaagaa atacccgatc ccccggcgac
     9661 aattacagga ttgttggaat acccattctc ccgaaccgag gtatcaaagg gaacaagcaa
     9721 ctctatacaa tgaaatcccg ggaacccaca aggatcggtt tcgggaccca caaaggaggt
     9781 gtcgtcatgc gtcgaagaag aggccctcct cctcccgccc gtggagagga tgcctaccgg
     9841 atgaaagaaa gggacctgcc ccttgggcag ggatcctcga aaagaaaggt tcagtgcgga
     9901 aggaatccca taggggagcg ggacgctgcc cggaacacgg agccctcgga ccggccggaa
     9961 agtcctcgcc ccaggcccaa gtgcgttatg gggccggctg caatcgtgct aagcatcttg
    10021 ctctgtgctg gggggggaac cgccctgggc ggcagactta cggcacagga cctcaagagc
    10081 tgcccttcgt gtttcttttg cggtatggac cgaaagtgct ttgcctacag ccgtgtggtc
    10141 atccgctacg acgatcgcag caccgtgggg gcgtgcagta tcaactgtgc tgctctggac
    10201 cttgccaaga gcattgacag gaccccggtc aagcttctgg taggggacta caagaaaaga
    10261 tcgttgatcg atgcggaaag ggccgtctgg atcatcggag gggacaagcc gggggtgatg
    10321 agtgaacgtg ccaaatgggc cttcgcgagc agacgggatg ccgaagagta cattcgaaaa
    10381 cacgggggtg ccctggccgg ctttgatgag gtcatgatag cgacctatct ggacatctat
    10441 cgggagacgg tgaagtagga gtttcccgcc ctttgtgagg atttgcttgg attttccggc
    10501 cggcgatggt gtagactgaa ttccccgtca aggaccgtcc cctattcatc atgcacaaaa
    10561 ggaggtgagg tatgaagagg gcattcctgg ttcccctgat cctggccttt gccttgaccc
    10621 ttgtttcggt gtcctttgcc gaagagtccg gaaggctcga tctcaaggtg ggcgacgaag
    10681 tctacgtttg cggatgcggc aagggctgtc cctgcgacac catcgccatg aagccgggca
    10741 agtgcgtttg caagagaaag ctggtcaagg ccaaggtcgt caaggttgaa gaaggcaaag
    10801 ccaccatgct tgtcggcaag aaggaaacgg tgttcaaaac cattgggaaa tatgcctgtg
    10861 cctgcgggcc gggttgtgac tgcggcacca tcagccagct tccgggcaaa tgcgcctgcg
    10921 gcaaagccat gaaaaaagtc tcggacgtca agaagaagtc cacctgatgc ccctcctgtg
    10981 ccaccccccc ctgccgggtg gctcggggat tgtcggctcc cttcagccgt gcgactgctt
    11041 ttgcgcagac cagggggagt caccccgata aaagataaag ggtgttggag accgatggag
    11101 atcttcccgc acccttttct cgttcttcaa ggggcccgcc gggagcaggg caacttgtcc
    11161 tgaagaggcc tcccgggaga atgacggatc cgcatcacga ctctctggag tcttgatgcg
    11221 gatacgcccg gcatgagttt gaaaaaagtc cccgctgcat ttcggcgggt ctttcctact
    11281 ccttatcccg cttgtgtttt ccctgttgtc cttgctgctg acgctgctgc tgttgttgtt
    11341 gacgctgctg catctgctgc tggcgttgtt gctgttgttg ctggcgctgc tgcatctgct
    11401 gctgacgctg ctgctgttgt tgttgacgct gctgcatctg ctgctggcgt tnnnnnnnnn
    11461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctgttggc gttcttggcg ttgctgcatt
    11581 tgttgctgac gctgctgttg ttgttgacgc tgctgcatct gttgctggcg ttgctgctgt
    11641 tgttgctggc gctgctgctg gcgctcctgg cgttgctgca tttgttgcgg gcgctgttct
    11701 ctctgttgtt gacgctgttg cagttgctgc tggcgctgct gttggcgttg ttgcctcacg
    11761 tcctgaccct ggggctgacg ttcctgtcgt ctttgccgcc gctcctcctg catctgttgg
    11821 cgtcgttccc gctgctgctg ttgttggact tcacgtttct gtctgcggtc ctgatccatt
    11881 tgctgtcggc gttcttcccg ggtcctgccc cgatcgtccc ttcgagcctt ttcccgctcg
    11941 aggcggcgtt cttcccgggc cttggcgcgc tcgtcgcgat ccatcctgcc ccgatcatcc
    12001 cttcgagcct tctcccgctc gatgcggcgt tcttcccggg ccttggcgcg ctcgtcccgg
    12061 tccatcctgc cccgatcatc ccttcgagcc ttctcccgct cgatctggtc cctcttgggg
    12121 ggccggatcc ggtcgcgatc ccccttgcgg tcctccctac ggtctcccgg tctgcgtaca
    12181 tcccgatctc gaacctgccg cccctgcttc ttcaagtccc tattggccac cttgggcttc
    12241 tgaagcctgg catctccgga cggtcttcct tctttaaggt tcttgaccct gcgttcataa
    12301 tccctggcgt tgattttgcg ctcctgtgcg gccagcttac ggttctgatt gatgcgattg
    12361 agatttgccc tcggttttcc tttatagacg gcattggtga aattgcgcct ggctcgacgc
    12421 gaatcgtaat tgttgatgac ggttttgttt atgaccttgg tagtcttgta gttgtttatg
    12481 atcaccgttt tattgatatt ccgaattctg acgctggagt accggtgaac tccccaaaaa
    12541 ttcacctggg ggaccaacac ggcgtggccc acataggcca gagaaccgat agcgaggccc
    12601 acacccaacg cggccacccc cacgcctccg accaccacgg cgcccgggaa ccatgggcga
    12661 tgacagtagt agggttccca cggagccagg ggtacccaac cgatatccgc ttcggaatga
    12721 atccaggcga ctctcccggg attccagcag cagcagcagc cgtaggacgg gcccacgtga
    12781 acccgaacca ccggaggcgc ccagtaccag caactgcagt catcgatgta ggcccaactg
    12841 ccgtaatgat gagtcgtata accgaagggc tcttcgggaa tccagcaatt gtcgtcgtac
    12901 cattccgtcc atcggccttc gctgtaagga gcccagtccg agtcaacgtg tgtcggccgc
    12961 cacatcatcc ggtgttcgcc gttgtagtag actcgatccc accttccgtt ttcctccagt
    13021 tcgtatgcct gatcatggag actctcgggg atgtatcgca ccgaatctcc cttgacctga
    13081 aggcgtcggc tccaaaggtt gtctcgttcc gtgttccaat cgtcccaggc gacgtccacg
    13141 gtgccgtcgt cagcagtcac ctggctgccg tcggaaacca gggattccga cccggcgatc
    13201 acttcgtact tgctctgatc cttctcggtg acgaaatcaa cggtcccacg gatggcaatg
    13261 atctcgagag actcatctcc cacgtaaagg tcgaagaccg ttgccgcccc tgcaagaacg
    13321 tatccgtacg gagtcgtcac gcggaccact ccgtccttgc tcttgttgta gaaccgcgcc
    13381 actccggagg ccacatccat ttccgtcaag tcgtcctgca aggagatgag ctggatctgg
    13441 gtgtcttctc ccatgcggac ttgggtgctg ttgggaagga ggagctcaac cctggagtcc
    13501 ttatcggaat agagggcgtc ctcgaagccg aaaggagcgt ccttgaccgt gatcacccaa
    13561 tcgtcgtctt cggggacata gcgcagcacc tgtccctcga cgtgtgaaat ccggccgacc
    13621 aggactccgc catcatcgcc tccctgggcc tgggcaagga cgcaaggcgc ggttgagagc
    13681 agaaccgcaa gcgccagtat tgagaatctc gccagcctta tcatacccgt tcctttctaa
    13741 gcctgtaatg gtggttgaag agttgagcgt ttacgagatg ttgaccgact atatggaaag
    13801 tcacacgacg tgatcacccc tctcatggtt cccccctaat aacccataca gacgtttgtg
    13861 aagctggaac atattacaaa acattgagat tcgttgccat ataattcgtt tgcccaaccg
    13921 ccaagcgaca gctcggaccc cagccccgag atcgggcatg cgcagacgag tcgtggatcc
    13981 tgatcgccga ccaaggcgcc ctttcgtgca gggtcccccg ggcaaaggcc gggaagcggt
    14041 ccagtttccc ctttcctatc cctcgggaat ttgccatcat cgggattgaa aggagggaca
    14101 ccctatgcaa agacaagaga cacccgagcg tctcaccgtg aaggccgcgg caaggaccct
    14161 gaacgtgtct accaagacca ttcatcgata tcttgcaaaa ggctttctaa caaagattaa
    14221 ggagggtacc cggaccctcg tctccctcca ggaactgacg gcatatctgg gacgggtgtc
    14281 tcagggacag ggaccaggac tggaggggac acaacccagg acacaaaagg gacagagtgg
    14341 ggacacggtg accctggatc gagagcgtta tgaaagtctt ctcatggaaa tcggggaact
    14401 gaggaagggg agccggctca tgggggagct caagcaactc gtggagggaa gagatcaggg
    14461 ctcggggccg gacagaaaag aactggagga gctggcggcc cgcgtccagt acttggagtt
    14521 gcggggcctg gaaagagaga agcaatgggt cgagctcttg tctcgggtga aggacctgga
    14581 ggccgacctc tcgaggatga agatgcagaa gccgtggtgg caaaagtagc aggatcagca
    14641 acgggggaca aaggacccgg gcttggggag tccggaaaaa ccctcggtcc cgactccggg
    14701 cacttatccg ggcccgcccc gtcatccggc gccgagggag gcatgggggg cgtcggggga
    14761 cccgagttgt cgaggcaatc cccccggggc tccccggagt cccctaccct ctttccttca
    14821 ccgcttttcc gatcttcctc ccgaattcca gacatcgttg caggtcgtca tgggtcggtg
    14881 cgtagtggac ccgtattccc ggttcgagca catcgaattt catttccttg agtgcgtcgt
    14941 tgatcacctt caccgcttcc ccactccatc cgtaagagcc gaaagcggcc gcgatcttgt
    15001 tcacgggacg cagtcccttc atataacaaa gcagatcggc catggtgggt aggattccgt
    15061 tgttgagggt gggagaaccc agaatcaagg ccccggcatc caggacttcc gtgatgatgt
    15121 cgcttcgatg gttggtcttg aggttgagaa tccgcacgct gaccccttcc tccataagac
    15181 ctttggcgat ggatcgagcc atgatctcgg tgctttgcca catggtatcg tagatgagca
    15241 aggccttgct cttcggcttc tggaggctcc atgttgcgta ggagtccaga attttcgaca
    15301 cattcgaccg ccagatcaat ccatggtcgg gagcgatcat gtcgatggcc agacccagct
    15361 ccttgacctt tgccaggacc ttctgaacca agggcgagta cgggagcaga atgttggcat
    15421 agtacctggc tgcgtggtgg aagagttcgg gcaagtccac ttggtcgtcg aagcgttcac
    15481 tggtggccca gtgctggccg aaaccgtcgc tggagatcaa caggcgggcc tcggggacgt
    15541 aggagagcat gctgtcgggc cagtgcaaca tgcgggtttc gatgaactgc acggaccgcc
    15601 tccccagggg gatattcctg ccggtttcca cgacttcgta gggccagtct tccctgtggt
    15661 agtgctgaat cagggccttt ttccccatgg gggaacagat gagcttttcg ggccggatga
    15721 gatccaccat ctccggcaca catcccgaat gatccatttc cacatgattg actatgaggt
    15781 aatcgatcct ttgcggctcg acgaccttcc ggatattgtg aatcaaagtg tccttgaagg
    15841 gcttcttgac cgtgtcgaag agggcgacct tttcgtccat gaggaggaaa gcgttgtagg
    15901 tcgtaccctt ggggacggaa tagccgtgga agtcccggat attccaatcc acggctccaa
    15961 cccagtaaag gtccttcagt acctcgaaag attgcatgac taccccccct aacgagtgag
    16021 caaatcggac taccggctca tttcagtcgc atcccgtgcc gggcaccggt ttgaacatct
    16081 ttttgcctgc cccgcaaacg gggcatttcc agtcttcggg aagatcgtcg aaggaggtcc
    16141 ccttttcgat tttccccttt cggtcccctc tcttggggtc atagaggtat ccgcagtttg
    16201 tggtttggca ttgataaagg caatcggaac cgcccatagt tttccctttc tccggatgaa
    16261 attcgctgca gccgcgggga gcccctacca gttttctccc aggatctcgt aatgagctcg
    16321 aggatgtgcg catgcgggac agagctccgg ggcttcagcc ccctcgtgaa ggtagccgca
    16381 gttgatgcat cgccagatga cggcggtttc cctttgaaag actcgatttg cggaaatatt
    16441 gccggccagg gccttgtacc tcttctcatg ctgtttctct gctacggcga tggcctcgaa
    16501 gacctccgcg atttccggga gaccttcctc ccgggcaacg gccgcgaatt ccggatacat
    16561 gaccgtgtgc tcgtgattct cgccggccgc cgcttcccgg aggttctcga gggtgcttcc
    16621 aatgcgccct gcgggatagg tcgccgtaac ggacacctcg cccccttcca gaaacttgaa
    16681 gagcctcttg gcgtgttcct tttcctggtt cgccgtctct tcgaaaatag cggcgatttg
    16741 cacgaagccg tccttctttg cctggctgga aaaatacgtg taccggtttc tggcctggga
    16801 ttcccccgaa aatgctgtga gaaggttctt ttccgtcctg gtgccgacta gtttgggcat
    16861 gttatcctcc cctctgagag tcacgaattc ctgcctgggt tttacggctt cggtaccgct
    16921 atcgagaata agccatggtg agtcgaagaa ccactactaa ctaatcagca ggggttctat
    16981 tgtcaatgcc gctcccggca tgctaccatg aaagccaatc caatttgtcc ctggaaggag
    17041 aacggtgagc aggtcttcct cgatcccaga cccagtcaag gagcaggagt tgcaggaaga
    17101 agaaaaccgc cttcgtcgtc tgcgaaggct ggtggatttt tcccttgccc tcatcgccca
    17161 gtcggacctc gctctggaag aggctcagaa ccttcatcgg atggtgcgca acacggcatc
    17221 ccgactgttt cccggcaaag aagccgcctt cgatctcatc tatacccctc gttttcgtcg
    17281 cctcattgcg gaaaagtatg ctttacagta aaaaggggaa ggcaatccgg aaccgatttc
    17341 ggattgcccc cggagatgcc tttgcatacg ctctaccctg cggagggacc cttcttcgag
    17401 gggggcccga cacgaaaggc gaccgtcggt ctatggatct atggatttta aaacttcagc
    17461 gggaagcgac gaggatcgca tccagacatc gcattccgga attctacgtt cgattcaagg
    17521 cccccatggc cgtggcacgc aagcttttct tcaccaactc ttcggtcgtg tatcttcgag
    17581 gcatggttcg accaatcctg caggacgaga tgggccacgg cctgctccac agcacgcggg
    17641 tcggtcttga tgcggcaacc ctcatcgatg tggaactgat gtccagcccc atgagccggg
    17701 aagaggtgga gcggctcatg gtgctgggac tggtggccgg gcttctccac gacatctgcc
    17761 gcaacgagag caaccatgcg gagtccgggg cactggaagc ggccagggtt ttgcagaacc
    17821 ttcccatgac ccgggaggag agccggtgca tctgcctggc gattcgaaac cacgaggctt
    17881 ttgtggcgcc ggttctc
//
LOCUS       DAOOVC010000074        17786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2178_length_17786_cov_5.910609, whole genome shotgun sequence.
ACCESSION   DAOOVC010000074 DAOOVC010000000
VERSION     DAOOVC010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17786
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2178_length_17786_cov_5.910609"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLM79_10540"
     CDS             <1..295
                     /locus_tag="PLM79_10540"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF015883.2"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOV86791.1"
                     /translation="VEAEVRLYDRLFAVEDPSDGEEGIHFKDYLNPRSLEVLTGCRLE
                     PSLKAAEPGSLYQFERMGYFCADSRDCRPGSLVFNRTATLRDTWAKIRQKIQG"
     gene            518..736
                     /locus_tag="PLM79_10545"
     CDS             518..736
                     /locus_tag="PLM79_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86792.1"
                     /translation="MKKILSIFVAMAFAFTLSLPLTSFAQTQTGTKQEQTEKKKTKKK
                     KAKKATKKAPEKKSSKAKKSAPKAEEKQ"
     gene            1230..2561
                     /locus_tag="PLM79_10550"
     CDS             1230..2561
                     /locus_tag="PLM79_10550"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOV86793.1"
                     /translation="MVSCHTPEDVLRLTSERQVSFIQLWFTDVLGQLKSFSVTSDELP
                     LAFREGMGFDGSSIEGFARIEESDMVAVPDPTTFQFLPWRPQEKPVGRMFCDILNPDG
                     SPYEGDPRYVLKRMLRKAAEKGFTLYVGPELEYFYFKSDSAPEIIDRGGYFDVTPLDG
                     GNDLRRQTIFALQQMGIQVEYSHHEVAPSQHEIDLRYDEALRMADKTQTYRIAVKEVA
                     RQNGVYATFMPKPLFGQNGSGMHTHQSLFLGERNAFFDPTDRFHLSRLARQYTAGILK
                     HAKEIIAITNQWVNSYKRLVPGYEAPVYISWARRNRSTMVRIPMYKPGKEKATRIEFR
                     APDPACNPYLAFAVMLAAGLRGIEREYELPDPIEEDIFEMSPEERERRGIHSLPGSLY
                     EAIWACERSELVRETVGAHIFQKFIENKKIEWDLYRIQVSGYEIERYLPIF"
     gene            complement(2636..5389)
                     /locus_tag="PLM79_10555"
     CDS             complement(2636..5389)
                     /locus_tag="PLM79_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOV86794.1"
                     /translation="MERLMAKHWHHIAGAEVVDLLQTDPDRGLDEFEVKHRQERFGPN
                     LITGKGGKGPLVRLLLQFHQPLIYILLAAGAVTAALGEWVDSGVIFGVVLVNAAIGFL
                     QESKALEAIAALARTMVAETTVLRAGLKRRISFTEVVPGDLVLLQPGDKVPADMRLIE
                     IRDLQVDESSLTGESVPVKKKRGTLGPDTVLADRRNMAYASTLVTYGQGSGVVVAIGN
                     ATEVGRISSLIAAAPDLQTPLTRKIGHFSHLLLYAILALAGITVLIGILRGQPLLEMF
                     MAAVALAVGAIPEGLPAALSITLAIGVARMAKRRAVIRRLPAVETLGSTTVICSDKTG
                     TLTENQMTVQAISAGGEAFEVTGTGYEPSGTVLRQGEPVTPGQFPVLMECLRAGLLCN
                     DSVLLEKDGRWEVQGDPTEGALLVSAAKAGLSAREAAVQFPRLDVIPFESERQYMATL
                     HTGEQAGPQVAYVKGAAEILLDRCTEALDAAGARTRLDRDWVMSEVERMASGGLRVLA
                     FAKKEISPESAPFDHEDVADGLCFLGLQGMIDPPRAEAITAVKACHTAGIRVKMITGD
                     HAVTASAIAERIGLVESSTSSPAKETAITGRALAGMPDRELIEVVEKTSVFARVDPEQ
                     KLRLVEALQARGHIVAMTGDGVNDAPALKRANIGIAMGITGTEVAKEAADMVLTDDNF
                     ATIEAAVEEGRGVFDNLTKFIVWTLPTNLGEGLVILVAILAGTTLPILPVQILWINMV
                     TAVLLGLTLAFEPKEPGIMLRPPRDPKTPILTCYLLWRILLVGGMLCAAAFGLFEREL
                     HRGASEAQARTVAVNVFVAAEAFYLLNCRSLTKSMFQLGPFSNPWLFVGVTLMALLQV
                     LYTYVPAMNWMFHGAPIPPAAWGSVLAAGLLVYLVISFEKWIRLRLTASGQATETATG
                     SSG"
     gene            complement(5669..6895)
                     /locus_tag="PLM79_10560"
     CDS             complement(5669..6895)
                     /locus_tag="PLM79_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOV86795.1"
                     /translation="MRNAFGRIPAGHPDRRGSCRARAPWTRLPAGLAIMLLWIVWTVS
                     AAQGASLFRSVVTFGDSLTHNDVLGIAYDNPQSLYGRDPMEAFFDRAASGGDELASFA
                     VAGSKAGNLKAQLAAYAAAVELDLVRKGSLFSIEIGGNDLMDEIHRLARNPPRVSPAT
                     DRIINRIVNSMRSASAFLRKTHPQARILLWTLPDVTRIPKFWDEFSPQQQENIRAHIL
                     RGNRAIRRLAADSRVIILDLHEVLRRTTETPPILLGRPLLPPPAWGEYDCVFADPIHP
                     TAVANALIANEAVQTIRTAWGLTIPLFNERQLARMAHIRSALAPTDSPLLHYETSRPA
                     AITGELLPWSLDYGPPTPSSRGEDDTRTAGPAGKRADLRLKSRPDYVRCVLTPGDIGA
                     VSALKNPEGRSGRTGV"
     gene            complement(6955..8031)
                     /locus_tag="PLM79_10565"
     CDS             complement(6955..8031)
                     /locus_tag="PLM79_10565"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HOV86796.1"
                     /translation="MSRILLIDVGAGTMDVLFHDMESGLQCKAVVRSPVLTVAEQITR
                     TPGNLLIKGVEMGGGSLAAILRQRALRDEVLISASAAMTLHHQEDRVRSWGIRVIPDA
                     EADEFRPTAGFSVLTFADLDRERLRSLFSCFGVPFEMDSVGVCAQDHGIPPPGSGHLD
                     HRHRVFAEALDRDPFPHALLFPHDEIPGTFHRLQSIARCAMNLPTPEAWVMDSGMAAI
                     LGASMDSSARRAERVLVLDAATSHTVGAALDRGEITGFFEYHTRDVTLPRIESLLEAV
                     ADGKLRHSRILEEGGHGAYIRKALGFDRVQDIVATGPRRRILDGSRLSIRFAAPGGDN
                     MMTGNVGLLEAICRRKGLPSPQWI"
     gene            complement(8215..8547)
                     /locus_tag="PLM79_10570"
     CDS             complement(8215..8547)
                     /locus_tag="PLM79_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV86797.1"
                     /translation="MEVIAEKLDKALKLKVKGRMDAITAPDFEKECAAWIEGGENDFI
                     VDLGELEYISSAGLRSVLITAKRLKTNNGRICFCNTTGMVQKVFSISGFQSMFPMYDT
                     LEEALAHR"
     gene            complement(8538..10505)
                     /locus_tag="PLM79_10575"
     CDS             complement(8538..10505)
                     /locus_tag="PLM79_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOV86798.1"
                     /translation="MFRDKGLAFKLILFILVCTVSIFGVAFAYDYYTSRDTVLKNVEE
                     NAANLTLSTVHQIESILKSVERVSLNTAALIEQQSFTAPELVSLVETMVSRNREIYGM
                     TVSFEPYAFDPESLYFAPYTCRTPDGRIRSYPPEYFAYPYFYWDWYQLPKELGRPVWT
                     EPYFDEGAGNAVMSTFSTPFYRHRDGGNRFQGVSTADLSLEWLADVVRQVKIYETGYA
                     FLISQNGVFVTHPHTELIMRRSIFSIAEERLDYRLREVGRAMIRGERGFVSITDFVSG
                     KKSWLYYAPLPSSKWAIGVIFPEDELFADVNQLGRQVLTIGGVGFLVLAFIVAWIAQT
                     ITRPLRSLADTTVAIAHGNLDIDLPEFPSRDEIGKLTNSFRDMRAALKEYIQNLAATT
                     AAKERMESELKIARVIQMSFLPKQFPRLSHRYSLEISAKLEPAREIGGDFYDYFLMDS
                     SHLLVTAGDVSDKGIPAALFMAVTKTLIKGMAEPGMEPSTILEKVNRELCLENESMMF
                     VTLFCGILDLETGEFRYSNAGHCPPILLPSRETPAWLDTPAGFVLGVDEDSSYETSGI
                     FLAPGDGIFLYTDGVTEAKSASNNFFSEQGLLELVRSNGEASADELVHTVFSTVTDFC
                     AGVPLFDDVTILAVRYRGTKHPQNGGLETWK"
     gene            complement(10492..11556)
                     /locus_tag="PLM79_10580"
     CDS             complement(10492..11556)
                     /locus_tag="PLM79_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV86799.1"
                     /translation="MVKRSFIYAALLFLLSGLTVRAETTAARPFVFLPQWHPQAQFAG
                     YYVALEKGFYKDRGIDLKILRGGPDRPVTDLLEKGIADFCTMFLSTAIQRRSGGMKLV
                     NIGQIMQHSSLMLVAKKSSGISSPRDMNGKRVGLWGADFQIQPRALFQRQGISPIIVP
                     QTTTMNLFLRGGVDVASATWYNEYHILLNSGLSPDELNVFFFHSYGLDFPEDGIYCME
                     KVLEENPEACCAFVRATIDGWLYAFAHPEEALDIVMKVVNENTLPTDRIHQRWMLGRM
                     YELICPVLPTLASGGPADVPYMQGRKEPSPAPQKGCPMGRLEEEDYNTVVRVLTAAGL
                     IQEVPRYSDFHRKCESDVQR"
     gene            12007..13815
                     /locus_tag="PLM79_10585"
     CDS             12007..13815
                     /locus_tag="PLM79_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOV86800.1"
                     /translation="MKKERISGLMRCNHGEEKLELRKLLSELAADGWISRHDSQRVLS
                     QAREKDLENIHPLVYLSDRRLTRPSPPHELLTLEGLTGWLARKVELPYFRIDPLKIDV
                     AIVTSLVSYAYASRFKILPVEVNEREVVIATAEPFVTEWVEELERILRLKISRVIANP
                     SDISRYLVEFYALTESVKGAVWKADSGKIGGVVNLEQLVELGRAGKLDANDRYVVNIV
                     DWLLQYAFDQRASDIHLEPRRDTSDVRFRIDGVLHAVYQLPTVVMGAVVSRLKVLGRM
                     DLAEKRRPQDGRIKTKTQAGAEVELRLSTMPTVFGEKLVMRIFDPQVTVRSFEELGFD
                     DHELKSWNRMVNSPYGIILVTGPTGSGKTTTLYSTLKQLARPEVNVCTVEEPIEMVEP
                     RFNQMQVQPSIGLDFAGGLRNLLRQDPDIIMVGEIRDLETAEMAVQAALTGHLVFSTL
                     HTNDAVSAIVRLMDIGVPAYLIRATLLGVVAQRLVRTLCPHCKRPGTIEPQLWQSLIH
                     PWKMDPPARAFHPRGCLECRETGYLGRVGLYEILTTSATLRPLIRTESDVARLRDQAV
                     REGMLPLQISGAKKICSGITTLEEVFRVAGSSDQMQ"
     gene            complement(13844..17572)
                     /locus_tag="PLM79_10590"
     CDS             complement(13844..17572)
                     /locus_tag="PLM79_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86801.1"
                     /translation="MKFLKAWIIPAFLFGVCVFLLQSAYTEVKRKSIEQLNVEQRLLA
                     RQAAKGIESFFDHYLHLLQGLSRVDHVIAADEEGLRMMHLFLEAHGGDLQGIVRLDGS
                     GKILRMLPSDTDLTDLSYREHVQTLLQTRQPVVSEVFASNPGGQSVAIHVPVYKDSRF
                     DGSLGFLIPFDRLARKYLEDIRLMKDGYAWVISRSGIELFCPVPGHIGNSVFDNCKDF
                     PSILDMAREMVKGKEGTTTYVFDKLRGESTEAVVKHAVYMPVRLHNTFWSIVVATPEH
                     EILESIQGLRDRWLLIAGILGVSGVFWLFGFVRAYLLAHEVSRRRKTEADLLESEKRY
                     RQLVELAQEGIWVTDVAGNTTFVNPRMAEMLGYSPEEMSGRPFCAFAAPSAVEDCRTY
                     MEREQEGTRTQWELEFLRKDGTPVIGSLSTCSILDDQGNPVGSLALVADVTQHRRAEA
                     GIRESRLQMMRILEGFPVPAFFIGKDHRVIYWNRAIEELTGIRSETVIGTNEHWRAFY
                     AAERPCMADLLADETPEALSRLYEGKYGPWKLLEEAYEATDFFPALGSKGKWLHFTAV
                     AIRDSQGALVGVIETLQDITERKRTEDALQESRQRLADIIDFLPDATFVVDREGRVIA
                     WNRAMEEMTGVPASHMLGKGDFEYALPFYGERRPLLIDLVLKPDPAFESLYDPIDRSA
                     SVLSGEAYFPALRGSEAYLLGKASVLSDSKGNVVGAIESIRDITRRKKAEAALRIAEE
                     KYRSIFENAIEGIYQTTLDGRFLSVNPAFAGMLGYDSPEELIGAMTDIRTQLYANPNH
                     RTRLLREIEARGAVRDFEVRFLRKDGKPAWVSLNVHGVRDETGTIRHIEGSAQDITER
                     RLLEARLAQAHRMEAIGTLAGGIAHDFNNMLSPILGYSELSLLEIPENTRLRHNIEQV
                     IQAAYRARDLVKQILTFSRNTPGELKPVRPGMIIREALGLLRPSLPSTIEIHTNLDED
                     AVHSTVIADSTGIHQVLMNLCTNAAHAMRETGGVLTIGLDDVDLDADTARQHQDLKPG
                     PYVRLSVSDTGHGMEDSVKERIFDPYFTTKGPSEGSGMGLAVVYGIVKSLSGGIEVFS
                     RPGRGTTFEVYLPRSGTSPEPIRDSLPGILPGKGRILVVDDEKVLVEMIKEMLESLGY
                     EVSVRYTSMGALEFFRDHSESFDAVITDQTMPHMTGIQLAREILKIRGDVPVILCTGF
                     SEIIDEETAQHYGIKAFLMKPVAMQQLAEVVRTVLNRE"
BASE COUNT         3494 a         5349 c         5514 g         3429 t
ORIGIN      
        1 ggtggaggcg gaggtgaggc tctacgaccg cctgtttgcc gtcgaggacc ccagcgatgg
       61 ggaggagggg attcatttca aggattacct gaacccccgt tctctcgaag tcctcaccgg
      121 atgccggttg gagccgtcgc tgaaggccgc cgaaccgggg agcctctacc agttcgagcg
      181 catggggtat ttttgcgcgg attcccgcga ttgtcgtccc ggctcccttg tcttcaatcg
      241 tacggcgacc ttgcgggaca cctgggccaa gatccggcaa aaaatacagg ggtagggaag
      301 gggaaagtct cgatcctcgg attcgctccc gccgggtgcg gccggaaacg accgtctccc
      361 ccgggcattc gatcctcctg cccgtagaat caggaattcc gaagctcaga atcagggttt
      421 cggaaataga caggacggag gggaccgggt taagctgtgc gcggtttttg tcgagggatt
      481 ctcaaagaag ccgcatccat aacgaaaggg accaagcatg aaaaaaattc tttccatctt
      541 cgtggcaatg gcattcgcat tcaccctgtc gcttccgcta acctcctttg cccagaccca
      601 gacgggtacc aagcaggaac aaaccgaaaa gaagaagacc aaaaagaaga aggccaagaa
      661 ggccaccaag aaagccccgg aaaagaagtc ttccaaggct aagaaatccg ctcccaaggc
      721 tgaagaaaag caataatccc ccgatcccgc aacctgcttc tttgaggaaa tctcctcccc
      781 acttcccggc ccgggagtgg ggaggagttc tcttgggccc ccgggccgca gaacccaatc
      841 ccgcaccttc tcccgatctc gggtctcccc ggggcaacct ttccccccca tccgggaagt
      901 cttcctcatt tctccctgga ctgatcgctt tggccggtac gaatcccgtt tggtcatccg
      961 gcgcaggcgg aaatcgactg agagcgtttc cacccgctgg atgccgccgt cgcgggcatg
     1021 acgggttgat gctctcggaa tcgatccact cgggttctcc tctctttcgt cctttccctc
     1081 tctccccgcc gggccccaag accggcctcc cgaggggcac cccgactcgg gtcgacattg
     1141 ccgttggagg gggcttcctt tgatggtagt atcccatcgt tgagtgctta cactccattt
     1201 caacggtttc gtgcccaagg aggagaagaa tggtctcatg tcatacgccg gaggacgttc
     1261 tgaggctgac cagcgagagg caggtgtcgt tcatccagtt gtggttcacc gatgtcctgg
     1321 gacagctcaa gagcttttcc gtgacctcgg acgaattgcc cctggcattc agggaaggga
     1381 tgggctttga cgggtcctcc atcgaagggt tcgcccgcat cgaggaaagt gacatggtgg
     1441 cggttcccga ccccacgacc tttcagtttc ttccgtggcg tccccaggaa aagcccgtgg
     1501 gaagaatgtt ctgcgacatc ctgaatcccg acggaagccc ctacgaggga gacccccggt
     1561 acgtattgaa acgcatgttg aggaaggcgg cggaaaaagg attcacccta tacgtgggcc
     1621 cagagctgga gtatttctac ttcaagtcgg attccgcccc cgaaatcatc gaccggggcg
     1681 gctacttcga cgtgactccc ctggacggtg gaaacgattt gcgacgccag accatttttg
     1741 ccctgcagca gatggggatc caggtggaat acagccacca cgaagtcgct cccagtcagc
     1801 acgagatcga tctgcgctac gacgaagccc tccgcatggc cgacaaaacc cagacctatc
     1861 gaatcgcggt gaaggaagtg gcgcgacaaa acggggtcta tgccaccttc atgcccaagc
     1921 ccctcttcgg ccagaacggc agcggcatgc acactcacca atccctgttc ctgggagaac
     1981 gaaacgcctt cttcgacccc accgaccggt tccacctctc caggctggcc cgccaataca
     2041 ccgcaggcat cctcaagcat gccaaggaaa tcatcgccat caccaaccag tgggtcaatt
     2101 cctacaagag gctcgtgccc ggctatgaag cgccggttta catctcctgg gcccgccgta
     2161 accgctccac catggttcga attcccatgt acaaacccgg gaaagaaaaa gcgacgcgga
     2221 tcgaattccg tgcgccggac cctgcctgca atccttacct ggcctttgcg gtgatgctgg
     2281 cggcgggtct tcgaggcatc gaaagggagt acgagctccc agaccccatc gaggaggaca
     2341 tttttgaaat gtccccggaa gagcgggaga ggcgaggaat ccattctctc cccgggagcc
     2401 tttacgaagc catatgggcc tgcgaacgaa gcgagctggt tcgcgagacc gtgggcgccc
     2461 acatcttcca aaagttcatc gaaaacaaga agatcgaatg ggacctgtac cgaatccagg
     2521 tgagcgggta cgagatcgaa cggtacctgc ccattttttg acgggccgcc cataagaagc
     2581 ggatccgtcc gaaacccggt cctgcagacc gcaaacggcg aaccgaacgg acctttcacc
     2641 cggaagagcc ggtcgccgtc tccgtcgcct gcccgctggc ggtgagtctt agccggatcc
     2701 atttctcgaa gctgatcacg aggtagacaa ggaggcctgc ggccaggacg gatccccatg
     2761 ctgcgggggg gatgggcgcg ccgtggaaca tccagttcat ggcgggcaca taggtgtaga
     2821 ggacctggag gagggccatg agggtgacgc ccacaaagag ccagggattg gaaaaggggc
     2881 ccagctgaaa catggacttg gtcaacgagc gacagttgag caggtagaag gcctcggcgg
     2941 ccacaaagac gttcaccgcc acggttcgcg cctgggcctc gctcgccccc cggtgcagtt
     3001 cccgttcgaa gaggccgaac gcggcggcac agagcatacc gcccaccagg aggatgcgcc
     3061 agaggagata gcacgtcagg atgggggtct tcggatcgcg ggggggccgc aacatgatgc
     3121 cgggctcttt cggctcgaag gcaagggtca ggcccagcag gacggcggtc accatgttga
     3181 tccacaggat ctggacgggg aggatgggca gggttgtccc tgcgaggatg gccacgagga
     3241 tcacgagacc ttcccccagg ttggtgggca gggtccatac aatgaacttg gtgaggttgt
     3301 cgaagacgcc ccgaccttcc tccactgcgg cttcgatggt ggcaaaattg tcgtcggtga
     3361 ggaccatatc ggcggcttcc ttggccactt cggtgccggt aatccccata gcgatgccga
     3421 tattggcccg tttgagcgcc ggggcgtcgt tcaccccatc tcccgtcatg gccacgatgt
     3481 gtccccgggc ctggagcgcc tccaccaggc gcagcttctg ctccgggtcc acccgggcaa
     3541 aaaccgacgt cttttccacg acctcgatga gttcccggtc cggcatcccg gccagggctc
     3601 ttcccgtgat tgcggtctct ttcgccgggg aagaagtgct gctttcgacc aggccgattc
     3661 tttcggcaat ggccgatgcc gtgacggcgt ggtcccccgt gatcatcttg acgcggatgc
     3721 ccgccgtatg acaggccttc acggccgtga tcgcctccgc ccgcggcggg tcgatcattc
     3781 cctgcaaacc caggaagcaa agaccgtccg ccacatcttc gtggtcgaag ggagccgact
     3841 cgggagagat ttcctttttg gcgaaagcca gcacgcgcag gccgccggaa gccatcctct
     3901 cgacctcact catcacccag tctctgtcca accgagtgcg ggcgccggcg gcgtcaaggg
     3961 cttccgtaca ccggtcgagg aggatctccg ccgccccttt gacgtaggcg acttggggtc
     4021 cggcctgctc ccccgtatgc aatgtcgcca tgtactgacg ttcggattca aaggggatca
     4081 catccagccg cggaaactgc accgccgctt cccgggccga aaggcccgct ttggcagcgg
     4141 aaaccagcag cgccccctcg gtggggtccc cctgcacctc ccaacggcca tccttttcca
     4201 ggaggacgct gtcgttacac agcagcccgg cccgcaggca ttccatcagc accgggaact
     4261 ggccgggagt gacgggttcg ccctgcctca gaacggttcc cgaaggttcg taccctgtcc
     4321 ctgtgacttc gaaagcttcc cccccggcac tgatggcctg gacggtcatc tggttttcgg
     4381 tcaaagttcc cgttttgtcc gaacagatga cggtggtgct gcccagcgtc tccacggcgg
     4441 ggagcctgcg gatgacggcc cgccgcttgg ccatgcgagc gaccccgatg gcgagggtga
     4501 tgctcagggc agcggggagc ccctccggga tcgcgcccac ggccagtgcc accgccgcca
     4561 tgaacatctc gaggagaggt tggccccgca ggataccgat cagcaccgtg atccccgcca
     4621 aggccaggat ggcatagagc agaaggtggc tgaaatggcc gatcttgcgc gtgagcgggg
     4681 tctgaaggtc cggtgccgcc gcaatgagcg aggaaatgcg cccgacttcc gtggcattgc
     4741 cgatggcgac caccacgccg ctcccctgac cgtaggtcac cagggtcgag gcgtacgcca
     4801 tattgcgacg atccgccaga acggtgtcgg ggccgagggt tcctcgtttc tttttgaccg
     4861 ggaccgactc gccggtcagg ctcgattcat ccacctgaag gtcccggatc tcgatgaggc
     4921 gcatatcggc gggcaccttg tcgcccggct gcagcaggac gagatcgccc ggcaccacct
     4981 ccgtgaagga gatgcggcgt ttgagcccgg cccgcagcac cgtagtctcg gccaccatgg
     5041 ttcgggcaag agcggcaatg gcttcgaggg ccttggactc ctgaagaaaa ccgatggcgg
     5101 cgttgaccag caccaccccg aaaatcacgc ccgagtcgac ccattcccca agggcggcgg
     5161 taaccgctcc ggctgccagg aggatgtaga tgagcggctg atggaactgc agcagcaggc
     5221 gcacgagggg gccttttccc cccttgcccg tgatgagatt gggaccaaag cgctcctggc
     5281 ggtgtttgac ctcgaattca tccaggcccc ggtccgggtc ggtctgcaaa agatcgacga
     5341 cctccgcgcc tgcaatgtgg tgccagtgtt ttgccatgag tctttccatg gaacggccct
     5401 ccttaggggg gttgaataag aatcggtgaa ccggtctctg ggcgattggg taagccggga
     5461 cagtggatcg gcggcggttt gaattgcgtg cgtcaggttc cggaaattgg atcagggcat
     5521 acggcgcctg ttttcgtcac gaatgcgccc tggccgacgc ctcgagccgc ggaagggcct
     5581 ccgggaacgc gtccctactc gtcggacgcg gccccgtcgg gccgcgaggt tgagcctcag
     5641 cccttttgct cctttacgag acccaggttc agacaccggt tcgtcctgag cgaccctcgg
     5701 ggttcttcag ggccgagacg gcaccaatgt cgcccggagt gagcacacag cgtacatagt
     5761 ccggccggct cttcaaccgg aggtccgccc ttttcccggc gggaccggca gtccgggtgt
     5821 cgtcctcacc tctcgacgac ggggtgggag gcccgtaatc tagggaccag ggtagcaatt
     5881 cgccggtgat ggcggccgga cgagaggtct cgtaatggag aaggggggag tccgtcgggg
     5941 cgagagcgct gcggatgtgg gccattctcg ccagttgccg ttcgttgaaa aggggaatcg
     6001 tcaatcccca agcggttctt atggtttgaa cggcttcgtt ggcaatgagg gcgtttgcca
     6061 cggcggtggg gtgaatggga tcggcaaaga cgcaatcgta ttctccccag gcgggaggcg
     6121 gcaggagtgg acggcccagg agaatgggag gcgtctccgt ggttcgcctg agcacctcgt
     6181 ggagatccag gatgatcacc cgggagtccg cggccagcct ccggatggcg cgattgcccc
     6241 gcagtatgtg agcccgaatg ttttcctgct gctggggact gaactcgtcc cagaacttgg
     6301 gaatccgcgt cacatcgggc aaggtccaga ggaggatccg ggcctgcgga tgggtcttgc
     6361 gcaggaatgc cgaggccgat ctcatggaat tcacgatccg gttgatgatc cggtccgtgg
     6421 cagggctcac tctcggggga tttcgagcca ggcgatggat ctcgtccatg aggtcgttgc
     6481 ccccgatctc gatgctgaac aggctgccct tgcggaccag gtccagttcg acggccgcgg
     6541 cataggcggc caattgggcc ttgagatttc cggccttgga tccggcgacg gcaaaggaag
     6601 ccagttcatc cccgcctgat gcggccctgt cgaaaaacgc ctccatgggg tccctgccgt
     6661 agaggctctg gggattgtcg taggcgatgc cgagcacatc gttgtgggtg agactgtctc
     6721 cgaaagtgac cacggaccgg aaaaggcttg cgccttgcgc ggccgaaacg gtccagacga
     6781 tccacaagag catgatcgcc aagccggcgg ggagccgggt ccatggagca cgggccctgc
     6841 atgatccgcg acgatcaggg tgcccggcgg ggatacgacc gaatgcgttt ctcatggttg
     6901 gaatcctcct tgcccgggtc ggtaaagctt cccggccttt ttccgtgcgg ggtctcatat
     6961 ccattgggga gaggggaggc cctttctgcg gcaaatggcc tcgagcagcc cgacattgcc
     7021 ggtcatcatg ttgtcccccc cgggtgccgc aaagcgaatg gacaggcgcg atccgtcaag
     7081 gatacgcctc ctgggtcccg tggcgacgat atcctggacc cggtcgaagc ccagggcctt
     7141 tcgaatgtaa gccccgtggc ctccttcttc aaggattcgg gagtgccgga gcttgccgtc
     7201 ggctacggcc tcgaggaggg actcgatgcg agggagggtc acatcccggg tgtggtactc
     7261 gaaaaagccc gtaatctcgc cccggtccag ggcggcaccc acggtgtggg aagtggccgc
     7321 gtccaatacg aggacccgtt ccgcccggcg agccgaagaa tccatggagg ctcccaggat
     7381 ggcggccatg ccgctgtcca tgacccaggc ctcgggggtg ggcaggttca tggcgcaccg
     7441 ggctatggat tggagccgat ggaaggtgcc tggaatttcg tcgtggggga agaggagagc
     7501 gtgggggaag ggatctcggt ccagagcctc ggcaaagact cgatggcggt gatccaggtg
     7561 accggatccg ggagggggaa tcccgtggtc ctgggcgcag actccgacgg agtccatttc
     7621 aaaggggacc ccgaaacagg agaacaggga ccggagtcgt tcccggtcca ggtccgcaaa
     7681 ggtcaaaacg ctgaaaccgg cggtggggcg aaactcgtcc gcctcggcgt ccggaatgac
     7741 ccgaatgccc caggagcgga cccggtcttc ctggtggtga agggtcatgg ccgcggaagc
     7801 ggaaataagg acctcgtccc gaagggccct ctggcggaga attgcggcca aggagccgcc
     7861 ccccatctcc acccccttga tgaggagatt ccccggagtc cgggttatct gctcggccac
     7921 ggtaagaacc ggggaacgca ccacggcctt gcattgcagg ccggattcca tgtcgtggaa
     7981 aagcacgtcc atggtgcccg cgcccacatc aatcaagaga atcctgctca cgagtctctc
     8041 cggtacatga acctgcggtc ctgtgggaac gacgttcgag gctcccggcg ggtgcaggac
     8101 ctgccgggag cctcccaacc gtcggacggg gcagggtgcc ttcccgcccg ggaaaagccg
     8161 cgcctccgga ctccccacgg ttgatgtcgc ccgattcgaa tgccgtctca cccatcagcg
     8221 gtgagccagc gcctcttcca gggtgtcgta catggggaac atggattgga accccgaaat
     8281 gctgaaaacc ttctgaacca ttcccgtcgt gttgcagaaa cagatccgcc cgttgttggt
     8341 ctttagtctc ttggcggtga tgagcacgct ccgaaggccc gcgctgctga tgtattcgag
     8401 ttcacccagg tccacgatga agtcgttttc gccgccctcg atccacgcgg cacattcttt
     8461 ttcgaagtcg ggggccgtga tggcgtccat gcggcctttg accttgagtt tcaatgcctt
     8521 gtccagcttc tcggcaatca cttccatgtt tcgagtcctc cgttttgggg gtgtttcgtc
     8581 cctctgtaac gaaccgcaag gatggtgacg tcgtcgaaca ggggcactcc ggcacaaaaa
     8641 tccgtcaccg tgctgaaaac cgtgtgcacc agttcgtcgg cggaagcctc cccgttggaa
     8701 cgaaccagtt ccaggaggcc ttgttcggag aaaaaattgt tcgaggcgct cttggcctcg
     8761 gtcactccgt cggtatacag gaaaatcccg tccccggggg ccaaaaagat cccggaagtt
     8821 tcgtaggacg agtcttcgtc cactcccagg acaaacccgg cgggggtatc gagccatgcg
     8881 ggggtttccc gggacggcag caggatgggc gggcagtgtc ccgcgttgga ataccggaac
     8941 tccccggtct ccaggtccag gatcccgcaa aagagggtca cgaacatcat ggattcgttt
     9001 tccaggcaca gttcccggtt gaccttctcg agaatggtcg agggttccat gcccggctcg
     9061 gccatcccct tgatgagagt cttggtgacg gccatgaaga gagctgcggg gatccccttg
     9121 tcggatacgt ctcccgcggt caccagcaag tgggaggaat ccatgagaaa gtagtcgtag
     9181 aaatcccctc cgatttcccg cgcgggctcc agcttggcgg agatctccag ggaatagcgg
     9241 tgggataggc ggggaaactg tttgggcaga aagctcattt gaatgacccg ggcgatcttg
     9301 agctcgctct ccatgcgttc cttggcggcg gtggtggccg ccaggttctg aatatactcc
     9361 ttcaaggcgg cgcgcatgtc ccggaaggag ttggtgagtt ttccgatctc gtcccgggaa
     9421 gggaattcgg gcaggtcgat atccaggttt ccgtgggcga tggccacggt ggtgtcggcc
     9481 agggacctca aaggcctggt gatggtctgg gcaatccacg ccacgatgaa ggccaggacc
     9541 aggaaaccga cacccccaat ggtgaggacc tggcgcccca attggttgac gtcggcgaaa
     9601 agctcgtcct cgggaaaaat gaccccgatg gcccacttgc tggaaggcag aggggcgtaa
     9661 tacagccagg atttctttcc ggaaacgaag tccgtaatgg acacgaatcc ccgctctccc
     9721 cggatcatgg cccgtcctac ctcccggagg cgatagtcga gcctttcttc ggcgatgctg
     9781 aaaatgctgc ggcgcatgat cagctccgta tgcgggtgag tcacgaacac cccgttttgg
     9841 gagatgagga atgcgtagcc cgtctcgtag atctttacct ggcggacgac gtcggccagc
     9901 cattccaggg agaggtccgc ggtcgagacg ccctggaacc ggtttccccc gtcccggtgc
     9961 cggtagaaag gagtcgagaa ggtggacatg acggcgttcc cggccccctc gtcgaaatac
    10021 ggctccgtcc aaacgggtcg gcccagttct ttgggcaact ggtaccaatc ccagtagaaa
    10081 tagggatagg caaagtactc gggaggatag ctccggatcc gtccgtcggg ggtgcggcag
    10141 gtgtaagggg cgaagtaaag ggactcggga tcgaaagcgt agggttcgaa cgagacggtc
    10201 atgccgtaaa tctcccggtt tcgagacacc atcgtctcca ccagggagac cagctccgga
    10261 gcggtaaaag actgttgttc gatgagggcc gccgtgttga gggacacccg ctccacgctc
    10321 ttgagaatgc tttcaatctg gtgaaccgtg gacagggtga ggtttgccgc gttttcctcc
    10381 acgttcttca gcacggtgtc ccgggacgtg taatagtcgt aggcgaacgc caccccgaag
    10441 atcgagacgg tgcataccag gatgaacagg atgagcttga aggcgaggcc tttatctctg
    10501 aacatcggat tcacacttcc tgtggaagtc ggaataacgg gggacctcct ggatgagtcc
    10561 cgcggcggtg aggacccgaa cgaccgtgtt gtagtcctct tcttcgaggc gacccatggg
    10621 gcatcccttt tggggggccg gtgagggttc ctttctgccc tgcatgtagg gaacatctgc
    10681 cggacctccg gacgcaaggg tgggaaggac cgggcagatc agctcgtaca ttctacccag
    10741 catccatcgc tggtggatgc gatccgtggg cagggtgttt tcattcacca ccttcatgac
    10801 gatgtccagg gcttcctcgg gatgggcaaa ggcgtagagc cacccgtcga tggtcgcacg
    10861 aacgaaggca caacaggcct cggggttctc ctcgaggacc ttctccatgc agtaaatccc
    10921 gtcttcgggg aagtccaggc cgtacgaatg aaagaagaag acgttgagct cgtcggggct
    10981 caggccggaa ttcaagagga tgtggtattc gttgtaccag gtggccgagg ccacatcgac
    11041 ccccccgcgc aggaagaggt tcatggtggt ggtttgaggg acgatgatgg gggagatccc
    11101 ctggcgctga aacagggccc ggggctgaat ttggaaatcg gccccccaca atcccacccg
    11161 ctttccgttc atgtcccggg gcgacgagat cccgctggat ttcttggcga ccagcatgag
    11221 ggaagaatgc tgcatgatct ggccgatgtt taccagcttc atccccccgg acctcctctg
    11281 gattgccgtg gaaagaaaca tggtgcaaaa gtcggcgatc cccttttcga gaaggtccgt
    11341 aaccggcctg tccgggccac cccgcaggat cttgagatcg attccccggt ctttgtaaaa
    11401 ccccttttcc aaagcaacgt agtagcctgc gaattgagcc tggggatgcc actggggaag
    11461 aaaaacaaaa ggacgagccg ccgtcgtctc ggctcgaacc gtgaggccgg agagcaaaaa
    11521 gagcagggcg gcgtaaatga agctcctttt caccatggcc tgccgggacc ttcctggggg
    11581 ccccctggaa tccggggctc gggagaaccg cccctccgcc gggcgccggg aaccgggcac
    11641 tgactgcgac tttcggcttg cgccgtgatg acgatcggct tcaaaggcat catagctccc
    11701 tcgcggtgtc gcactcctcc cgggaagaaa aggggcctct cccttcggag gtttcccccg
    11761 attcgttccc ctccggacca gttctgtata gcacattttc caaagttgag cgatgatccc
    11821 tcagcacccg cggccaatga aaatctttgg tcggccgggt tcggaccccg ggagccccgg
    11881 ccgggatggt cctgtgccgg agtccccgtt tgcgcctgca cggttttttt gcgaaaagaa
    11941 ccgttgacgg gcttttgagg ttccggcaga gtttctccac ggcaggtcca atgggagcga
    12001 tgggtcatga aaaaggaacg gatctcgggt ctcatgcgct gcaatcacgg agaggaaaaa
    12061 ctggagcttc gaaagctcct gagcgagctt gccgcagacg gctggatttc acggcacgac
    12121 tcccagcggg tcctttccca ggcaagggag aaggacctgg aaaacatcca tcccctggtc
    12181 tacctctcgg accgccggct gacccgccct tcgcctcccc atgaactcct gaccctggaa
    12241 gggttgaccg gatggctggc ccgcaaagtc gagcttccct attttcggat cgatcctttg
    12301 aagatcgatg tggccatcgt aaccagcttg gtttcttacg cctacgcttc ccgattcaag
    12361 atcctcccgg tggaagtgaa tgaacgggag gtggtgattg ccacggcgga accttttgtc
    12421 acggaatggg tggaggagtt ggagcgaatc ctgcggctca agatctcccg ggtcattgcc
    12481 aacccgtcgg acatctcccg ctacctggtc gagttctatg cattgaccga gtcggtgaaa
    12541 ggggcggtgt ggaaggcgga cagcggcaaa atcgggggag tggtcaacct cgagcagctg
    12601 gtggagctgg gccgggcggg caagctcgat gccaacgacc gctatgtggt caacattgtg
    12661 gactggctcc tccagtacgc cttcgatcag cgggccagcg acattcatct cgagccgaga
    12721 agggacacca gcgatgtgcg gtttcgcatc gacggggtcc ttcatgccgt gtaccagttg
    12781 cccacggtgg tgatgggagc cgtggtgagc cgcctcaagg tcttgggccg catggacctg
    12841 gcggaaaagc gccgccccca ggacggccgc atcaagacca agacccaggc gggggccgag
    12901 gtggaattga gattgtccac catgcccacg gtttttggcg aaaaactggt catgcgcatc
    12961 ttcgacccac aggtgaccgt gaggagcttc gaagagttgg ggttcgacga tcacgaactc
    13021 aagagctgga atcggatggt caacagccct tacgggatca tcctcgtcac cgggcctacg
    13081 ggttcgggca agaccaccac cctctattcc accctgaaac agctggcaag acccgaagtc
    13141 aacgtctgca cggtggagga acccatcgag atggtggagc cccggttcaa ccagatgcag
    13201 gtgcagccct ccatcggtct ggatttcgct ggggggttgc gcaacctcct gcgccaggac
    13261 ccggatatca tcatggtggg agaaatccgg gacctcgaga cggccgaaat ggcggtgcag
    13321 gccgccctga cgggccactt ggtcttttcc actctgcaca ccaacgatgc cgtctccgcc
    13381 attgtgcgcc tcatggacat cggggtaccg gcttatctca ttcgggcaac cctcctgggg
    13441 gttgtggccc agcggctggt gcgaaccctt tgcccccact gcaaacggcc gggcaccatc
    13501 gagccgcagc tctggcagtc cctcattcat ccctggaaga tggacccgcc cgcccgggcg
    13561 ttccaccccc gggggtgcct cgagtgtcgt gagacgggat acttgggtcg ggtcggcttg
    13621 tacgaaattc tcaccaccag tgcgaccctg cggcccttga tccggacgga atcggacgtg
    13681 gcccggctcc gggaccaggc ggtccgggaa ggtatgctgc cgctgcagat cagcggagcc
    13741 aagaaaatct gctcgggcat cacgaccctg gaagaggtct tccgcgtggc cggctccagc
    13801 gatcagatgc agtaggacga tagcccccgg agctcgaacc ggctcattcc cggttcaaga
    13861 cggtccggac cacctcggcc agctgctgca tggccacggg cttcatgaga aatgccttga
    13921 tcccgtaatg ctgggccgtc tcctcgtcaa tgatctcact gaaaccggtg cagaggatca
    13981 caggcacatc cccacggatt ttcaggattt cccgggccag ctggatgccc gtcatgtgag
    14041 gcatggtctg gtcggtaatg acggcatcga agctctcgga gtggtctcgg aagaactcga
    14101 gggcccccat gctcgtgtat cgcacggaca cttcatagcc caggctttcg agcatctctt
    14161 tgatcatctc cacgagcacc ttttcatcat ccaccacgag gattcgccct tttccgggaa
    14221 gaatgccggg gagagagtcc cggatgggtt ccgggctcgt tccgctcctg ggcaggtaga
    14281 cctcgaaggt tgtgccccgg cccggccggc tgaaaacctc gatcccgccg gacaggctct
    14341 tcacaatccc gtagactacc gccagtccca taccgctccc ttcactgggt cccttggtgg
    14401 tgaagtacgg atcaaaaatc cgctccttca cggaatcctc catgccgtgt cccgtgtcgg
    14461 aaaccgacag cctgacgtag ggtcccggct tcagatcctg gtgttgccgg gcggtgtcgg
    14521 catcgaggtc cacgtcgtcc agaccgatgg tcaacacgcc gcccgtctcg cgcatggcgt
    14581 gggcggcatt ggtgcaaagg ttcatgagga cctggtggat gccggtcgag tccgcaatga
    14641 cggtggaatg aaccgcatct tcatccaggt ttgtgtgaat ctcgatggtg gaaggaagag
    14701 agggacgaag cagccccagg gcttcccgaa tgatcatgcc cggacgcacc ggcttgagct
    14761 ccccgggggt gttgcggctg aaggtgagga tctgcttgac caggtccctg gcacgatagg
    14821 ctgcctggat gacctgctcg atgttgtggc gcaatcgcgt gttctcgggg atctccagca
    14881 gggagagctc ggaataaccc aggatgggtg agagcatatt gttgaaatca tgggcgatcc
    14941 cacctgccag ggtcccgatg gcctccattc tatgggcttg agccaggcgg gcttccagga
    15001 gtctgcgttc cgtgatgtct tgagcactgc cctcgatgtg gcggatggtc ccggtctcgt
    15061 cgcggacccc gtgaacattg agggaaaccc aggcgggttt cccgtctttg cggagaaacc
    15121 ggacctcgaa gtcccgcacc gcgccccggg cctcgatctc ccgaaggagc cgagtccggt
    15181 gattggggtt ggcgtagagc tgggtgcgga tgtccgtcat cgcgccgata agctcctcgg
    15241 gcgaatcgta tccgagcatt ccggcgaaag cgggattgac gctgagaaaa cggccgtcca
    15301 gggtggtctg gtagatgccc tctatggcgt tttcgaaaat gctccgatat ttctcttcag
    15361 cgatcctcag tgccgcctcg gccttcttgc gccgtgtgat gtctcggatg gattcgatgg
    15421 ctccaaccac gttccccttg gaatcggaca ggacgctggc cttccccaaa agataagcct
    15481 cggatcctcg aagagcagga aaataagcct cgccggacag gacggacgca ctcctgtcta
    15541 tgggatcgta cagggattca aaggccgggt ccggcttgag gacaagatcg atgaggagcg
    15601 gccttctctc cccgtagaac ggcagggcat attcgaaatc ccccttgccc agcatatggg
    15661 atgccgggac gccggtcatt tcttccatgg ccctgttcca ggcaatgacc ctgccttccc
    15721 gatccaccac gaaggttgcg tcgggtagga agtcgatgat gtccgccagc cgttgtcggg
    15781 attcctggag cgcgtcctcc gtccgcttac gttcggtgat gtcttggagg gtctcgatga
    15841 cgcccaccag agccccctgg gagtcccgga ttgcgacggc cgtaaagtga agccatttcc
    15901 ccttcgagcc caaagccggg aaaaaatccg tggcttcgta agcttcttcc agcagcttcc
    15961 acggcccata ctttccttca tacagccggg aaagggcttc gggggtctcg tccgccagta
    16021 ggtcggccat gcaggggcgc tccgccgcgt aaaaagcccg ccagtgttcg ttggtcccga
    16081 tcacggtttc cgaccgaatc cccgtgagtt cctcgatggc cctgttccaa tagatcaccc
    16141 ggtggtcctt cccaatgaag aaagccggga cgggaaaacc ctcgagaatg cgcatcatct
    16201 ggaggcggct ttctcggatg ccggcttcgg cccgtcggtg ctgggtcacg tcggccacca
    16261 aggccaggga tccaacggga ttcccctgat cgtccaggat ggagcaggtg ctcaggctgc
    16321 caatcaccgg agtcccgtcc tttcggagaa actcgagctc ccattgggtc cgggtgccct
    16381 cctgttcccg ttccatgtaa gtcctgcagt cctcgacggc ggagggggcc gcaaaagcac
    16441 aaaacggacg tcccgacatc tcctcgggcg aataccccag catctccgcc atccgaggat
    16501 tgacgaaggt ggtgttcccc gcaacgtccg tgacccaaat cccctcctgg gccaattcca
    16561 cgagttgccg gtagcgtttt tcgctctcaa ggagatccgc ctccgtcttt cggcgcctgg
    16621 agacctcgtg ggcgagcagg tacgcccgaa cgaaaccgaa gagccaaaac acgccgctga
    16681 cccccaggat cccggcgatg agcagccacc tgtccctgag tccctggatg ctttccagga
    16741 tttcgtgctc gggggtggcc accacgatgg accaaaacgt attatggagg cgaacgggca
    16801 tgtaaacggc gtgtttcacc accgcttccg tggattcgcc gcgtaacttg tcgaacacgt
    16861 aggtggtcgt cccctctttg cctttgacca tttcccgggc catgtccagg atggagggaa
    16921 aatccttgca gttgtcgaag accgagtttc cgatatgccc tgggaccggg caaaagagct
    16981 cgatcccgct ccggctgatc acccaggcat atccgtcctt catgagccga atgtcttcga
    17041 gatactttct cgcaagccga tcaaagggga tcagaaaccc caaggaaccg tcgaacctcg
    17101 aatctttgta taccggcaca tgaatcgcca cgctctgacc tcccggattg gacgcgaaaa
    17161 cttcgctcac cacgggttgt cgagtctgca gaagggtttg aacgtgttcg cggtaggaga
    17221 gatcggtcag gtccgtgtct gacgggagca tccggaggat tttcccgctt ccgtccaggc
    17281 gaacgatgcc ctggagatcc cctccgtggg cctccagaaa gaggtgcatc atccggagac
    17341 cttcctcgtc ggccgcgatg acgtgatcga cgcgggaaag cccttggagc agatggaggt
    17401 aatggtcgaa aaagctttca atgcccttgg ctgcttgcct ggccaggagt cgttgctcga
    17461 cgttgagctg ttcgatgctt ttccgtttca cttccgtgta ggcggactgg agaaggaaga
    17521 cgcacacacc aaacagaaac gccgggatga tccacgcttt caggaacttc acttcaacct
    17581 ccgtaacagg gacgcctgcg gaaacccatg atgccgcagg tcttggaaat cagcgaaaga
    17641 tatccatgat gtcgccgtgc gggcgaatcc gcgggatggg tgaccgggcc ggacgcaggg
    17701 gaccgccggc acggcaaaga tcgaggattg ccgaccggat acccgccttg gtccgaacca
    17761 cacccctccc cgggaatcgg ctccac
//
LOCUS       DAOOVC010000075        17722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2187_length_17722_cov_7.327220, whole genome shotgun sequence.
ACCESSION   DAOOVC010000075 DAOOVC010000000
VERSION     DAOOVC010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17722
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2187_length_17722_cov_7.327220"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLM79_10595"
     CDS             <1..260
                     /locus_tag="PLM79_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86802.1"
                     /translation="VLSTIRYFRDEYEAHILNKECPARVCRDLIRFKVNEEKCTKCGS
                     CYKACPVDAISWAKKQPAVIDTSKCMKCRSCILACRFHAID"
     gene            323..3037
                     /gene="fdhF"
                     /locus_tag="PLM79_10600"
     CDS             323..3037
                     /gene="fdhF"
                     /locus_tag="PLM79_10600"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1400..1402,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOV86803.1"
                     /translation="MDKDTITLTVNGKDFKGKKVQGKKGQTVLEVLRANDVYVPTLCF
                     HPKMPPYGGCRLCIVEIDNMRGLPPTCTTPAVDGMVVNTHTPKVVRVRKTVLELLVAY
                     GDHNCLLCEQTGNCELQNLVYEHGIDHIRYKTDFVPKPRDDSNPMIIRDHNKCVLCGR
                     CVRACLQVQMNGVIDVAARGSDAYITTFNNKPLMESSCVFCGECVQACPVGALTEKKA
                     YRLGRPWESTKVRTTCPYCGVGCQLWLHVKDGRITKVTGVEDGEPNRGRLCVKGRFGY
                     DFLYSEERLRTPLIRENGTFREASWDEALGLVASKFKEIIQKHGPDAIAGVSSSRSYN
                     EDSYQMQKLLRSVIGTNNVDNCARTUHAPTVAGLAKSFGSGAMTNSFADFAKAQMIFA
                     IGTNMTEAHPVASTFVKNAVEDGARLIVVDPRRTGLADWAQLHVSIKVGSDIAFLNAM
                     MNVLITEDLYDKEYVASNTVGFEELKAKVMEYPPEKAAPICGVDADVIRDVARRLASV
                     KPALLAYTLGITEHTCGMNNVLSCANLQMLLGNVGFEGGGVNPIRGQNNVQGACDMGA
                     LPNVFPGYQRVDDPKAQAKFKAAWKVDSLPEKPGLMMPQMMEGLADGRIKGFYIFGEN
                     LANSEPDIRHVEHLLASAEFLVCQDIFPTETTRFAHVILPAAAWSENEGTFTNSERRV
                     SRCRKVSEPPGISMPNWRIFKELARHMGHEWESNSAQELWDNEISVLAPALAGIKYAR
                     IENNGLQWPVPAQDHPGTPVLHKNGQFTSGKGRFVAVDWTPPAEVPDKEYPLVLSTGR
                     RLFHYHTRTQTGRAVGLNEILGEETADLSPQDAEALGIVDGEMIRVKSRRGEVKVKAK
                     VTDRMPEGMVWMAFHFREACANWLTNPAFDPVTQTAEYKACAVKIEKL"
     gene            3395..4513
                     /locus_tag="PLM79_10605"
     CDS             3395..4513
                     /locus_tag="PLM79_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV86804.1"
                     /translation="MEPANERLICNVKSIRKARGLSQTQLAKLVGVQRQAIYDMESGR
                     YMPNTSVALRLARHLQCKVEDLFSVEEPDLVQPVTLVGPAVQTGSRLRLGRVRNRLVG
                     FPLDGKEMLNDALLSADGLLDPQSGRIRLFQSASRLDRTALLLGCDPAFSILSAHVER
                     QTREARVVCRFSSSFRALEGVASGIAHVGGTHLHNSRQGEANLLLAQSALADTRATLL
                     AFSFFEEGLMVAAGNPLGIKSVGDLAGKDVRFVNREEGAALRILLDEHLARAGVRTES
                     IRGYNHLVSNHTQGALMVVYGLADAALGLRAVAAAHGLDFVPIEAVRCDLVVPVDLMD
                     HPAVALILDVLQTRSFREELASLPGYESSCTGKVVGNT"
     gene            4993..5673
                     /gene="modB"
                     /locus_tag="PLM79_10610"
     CDS             4993..5673
                     /gene="modB"
                     /locus_tag="PLM79_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOV86805.1"
                     /translation="MSQSLIPLLLTLKVAGFATLAAFLIGVSSAFLLTRVRFPGREWA
                     DAILTLPLVMPPTVMGYFLIVVFGRKGWIGHWLYDALGISLMFTWQGAVLASSVVSLP
                     LVFKAARSAFEGVDSNLERAARTLGLSETAVFFRVSLPLAWRGILAGTMLAFARAMGE
                     FGATLMVAGNLPGKTQTLSLAVYEAVQAGNDRLAATLVLITSLVCVVILVSSGKILKT
                     QYLKEVDR"
     gene            5670..6443
                     /gene="modA"
                     /locus_tag="PLM79_10615"
     CDS             5670..6443
                     /gene="modA"
                     /locus_tag="PLM79_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOV86806.1"
                     /translation="MKEKLLVVIAAALALLGMPPISPAKGMAEQELIVSAAASLTNAF
                     QEIAGKFAAANPGTKVIPNFAASGALLQQMEKGAPVDVFASADQKTMNQAQEKRLIFP
                     ETRKNFVSNGLVLILPVGSKTPVQGSRDLLAKEVTRVSLGNPETVPAGRYAREALENE
                     GLWEKLGSKLIPGDHVRQVLDYVARGEVDAGFVFATDAAQAKDKVRVVALLQQHQPIL
                     YPIAVAAATGKKDLAQRFVDFVLNREGQEILSRYGFGKP"
     gene            6445..7164
                     /locus_tag="PLM79_10620"
     CDS             6445..7164
                     /locus_tag="PLM79_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV86807.1"
                     /translation="MVIRVEVEKRMSSRAGQFELRVAFSSRDRCVVLFGPSGSGKTLT
                     LQSIAGLVTPDAGKIALNGQVLFDSQNGINVPSRHRNIGYVFQDYALFPHLTVMDNVG
                     FGLRRTWHRGLSREDRRQIMEFLGIFEISQLAGSFPADISGGQKQRVALARALIRKPD
                     LLLLDEPFSALDTLLRRRLREELVKIRHTFDLPMVMVTHDPEDIRVFAETLVVYEAGR
                     VRESRHFLMDNGIDLEEVLLA"
     gene            7334..7585
                     /locus_tag="PLM79_10625"
     CDS             7334..7585
                     /locus_tag="PLM79_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOV86808.1"
                     /translation="MQQCECEPPHPGEKHATEHPEYPERQGGRSEGRPGNGESEGRLG
                     GGNLIPSIVTVEAAQDLGGKVGDEVRVLIKATSIMIGKG"
     gene            complement(7817..8470)
                     /locus_tag="PLM79_10630"
     CDS             complement(7817..8470)
                     /locus_tag="PLM79_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HOV86809.1"
                     /translation="MASGKSILVDTSKCTACRGCQVACKQWNGLPGTKTEQLGNYQNP
                     QDFSADTYKVVRFADGKLENGKPYWYFFSDMCRHCLDPPCVDAIQGYVDGGAVKDEAT
                     GAVTYTDKSKEAPFDEVRSSCPFDVPRQNEKTKVLAKCTMCIDRITHDRPPACVKSCP
                     TGAMIFGERSQILDLAKKRVEELKKTHPKAEALKADEVRVIFVVTDEPKKYHKYAAG"
     gene            complement(8483..11533)
                     /gene="fdnG"
                     /locus_tag="PLM79_10635"
     CDS             complement(8483..11533)
                     /gene="fdnG"
                     /locus_tag="PLM79_10635"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(10946..10948),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HOV86810.1"
                     /translation="MGITRREFLTVSGAMGAGLALSSLGMDLGPIRAYAQEIRKVDKL
                     KTAKESTSICCYCSVGCGLLCSTDTKTNKILNIEGDPEHPISEGALCAKGAGIFQTTA
                     ANEHRLTKVLYRAPGSDKWQVKSWDWAIGEIAKKIKKTRDAHFIVKNSKGQVVNRVEA
                     IAHMGSSNVDNEECWAITAMVRALGLIHIDHQARVUHGPTVPALAESFGRGAMTNHFI
                     DLQHADVFLIQGSNAAENHPISFKWVTKAQERGAKVIHVDPRFTRTSARADVYAALRS
                     GTDIAFLGGMIKYILDNHRYFEEYVKNYTNASFLVDDKFGFDDGLFSGYDPNTRKYDK
                     VSWAIKRDAGGIPERDMTLQNPRCVFQLLKKHYSRYDLEKVSSITGTPVEDLKTVYEL
                     YSSTGVKDKAGTIMYALGQTHHTTGVQNIRTMCMVQLLLGNMGICGGGVNALRGEPNV
                     QGSTDFAILYHYLPGYLAAPRASQVTLEDYLKKNTPKSTDPRSANWWQNYPKYAVSLL
                     KSWYGDKAAKENDFGYEWVPKLDDGQDASVMYLFDQMYEKKIKGFMCIAQNPACSLPN
                     SNKVRKAMANLDWFVHVNIFDNETASFWKGPGMDPKKIKTEVFLLPASASVEKEGSQT
                     NSGRWVQWKYRAADPPGDAISTGDIVYRIMAKVKELYKKQKGGRFPEPILNLKWDYAD
                     EKGNFSALKVAKAVNGYFLEDVTVGDKVYKKGDCVPSFALLQADGKTSSGCWISCGSF
                     AQDGTNLMARRKKTDPTGLGLYPEWSWAWPVNRRIIYNRASVDLEGRPYNPQRAVLAW
                     KDGKWVGDVPDGPWPPMADAEKGKYPFIMKPDGVGSLFGPGLGDGPFPEHYEPVESPL
                     PKNLMSSQLNSPIIKLFKGDCDVYASCNPKYPFVMSTYTCTEHWCTGGITRWQSWLTE
                     AQPQAYVEMSLELAHLRGIKNGDKVKVESARGSLECVAMVTPRFRPFKVDGQVIHQVG
                     MTFNYGWLFPKDCGDSANLLTPTVGDGNTMTPEYKAFMVNVTKA"
     gene            complement(11548..11700)
                     /locus_tag="PLM79_10640"
     CDS             complement(11548..11700)
                     /locus_tag="PLM79_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86811.1"
                     /translation="MGFPREAVGARQGEGNRLLLFEGSAVRMSPGIGVFVKSMTENVE
                     WREFRP"
     gene            complement(11887..12249)
                     /locus_tag="PLM79_10645"
     CDS             complement(11887..12249)
                     /locus_tag="PLM79_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOV86812.1"
                     /translation="MKRPSPTVRLHLWLENGEGVYFGMGRAMLLLKVQELGSLKKAAD
                     QMGMSYRAAWGKIRKSEEILGIRLIASNGCRKEGCQLTEEGLRITEKFLRWFQEVEDD
                     ALRKAGEIFDCRVERYGS"
     gene            complement(12622..13197)
                     /locus_tag="PLM79_10650"
     CDS             complement(12622..13197)
                     /locus_tag="PLM79_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV86813.1"
                     /translation="MKVVAFNGSPRKDGNTTILLRCVLERLDGEGIGTELIQLSEKRI
                     EDCRSCYRCFENQDRECSNKEDDLNECIAKMVEAEGILLGSPTYFANVAPRMKALIDR
                     VGLVAFANPGMFRHKVGASLTAANRLGACHTLDAMNRFFYCFEMFLAGSNYPNMALGL
                     EKGDVEKDEVGMETMRLLGVNMALLLKKLHS"
     gene            complement(13624..13893)
                     /locus_tag="PLM79_10655"
     CDS             complement(13624..13893)
                     /locus_tag="PLM79_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86814.1"
                     /translation="MSMWFFDEMEESAEYLRLREEALQCEREYLEGMRALQEAERQLR
                     NHPDGENLRLRVEEIRGRLAEMEARCRWISSDVPMEMALWAPPHG"
     gene            14410..14961
                     /locus_tag="PLM79_10660"
     CDS             14410..14961
                     /locus_tag="PLM79_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV86815.1"
                     /translation="MIEIRFHGRGGQGAVTSAELTALAAIESGKHAQAFPSFGPERRG
                     APVMAFVRVSDTPISTREKVYEPNIVVVLDPTLLKLVNVEAGLRKGGMVIVNTTKSAE
                     ELRKETGIKAKLAVVDASKIAVETMRVPITNTTMLGALVKAAPEVISMESLAGPIDHR
                     FGPIAAKNMNSCKRAFDETKVEG"
     gene            14965..15258
                     /locus_tag="PLM79_10665"
     CDS             14965..15258
                     /locus_tag="PLM79_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86816.1"
                     /translation="MAKETGIISWKELNLGIAITTPGNAAELKTGDWAAQRPVTDREK
                     CTKCGQCYIFCPDMVYSKDAEGYYVQNYYHCKGCGICAKECPVDAITMVQEGD"
     gene            15260..16438
                     /locus_tag="PLM79_10670"
     CDS             15260..16438
                     /locus_tag="PLM79_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOV86817.1"
                     /translation="MSKHVGMEVSIAAAEAVGLCDVDVVAAYPITPQTHIVEHLSEMV
                     ADGHLDAEFVPVESEHSAMSCCIGSSAAGARTFTSTCSQGYALMSELCYIAASLRLPI
                     VMAVANRALSAPISIWNDHADLMYARDTGWIQLIGEFGQEVVDLMPIAFRIAEDKRVL
                     LPVQVNLDGFTLSHVIEPFLMPDKDEVKKFLPPFKPQLRLDPANPVTFGPVGMPDVYT
                     EARKAQDEALKNSKPVIQEIFDEWGKQFGRKYKLIETYKSEDADTIIVTMGSIAQTAM
                     TAIDHLRSQGKKVGLVRFRVFRPFPAKELFDAIKGAKNLVLLDRVSAFAGGEISAPVA
                     LEIKSVLYDRKHYPKVGNFLVGLGGRDVTVQNFEEMVERIAAAKDGELGAYEFINVRE
                     "
     gene            16439..17431
                     /gene="porB"
                     /locus_tag="PLM79_10675"
     CDS             16439..17431
                     /gene="porB"
                     /locus_tag="PLM79_10675"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HOV86818.1"
                     /translation="MGIASEALKNFKGFNLKNLPEVEPVSPGHRACQGCGEVLALRQA
                     MKAIGTNVVVCSATGCMEIITSPFPQTAWRVPWIHVAFENAAAVASGVEAAYKAMNRK
                     GIINQPDTVFVGYGGDGATADIGMQALSGALERGHNFIYMCFDNEAYMNTGIQRSSST
                     PYGAGTTTSPPGKKSIGQSTWKKNMSAIAVAHGVPYVATASPAYPIDLMNKAKKAAMV
                     PGPAYLHIYSPCPTGWRCGIEDGIEVAKLAVQSKVFPLYEVIDGVYYLSRKVAKPKPV
                     AEYFKPQRRFRHLTEAEVAKIQARVDQEYEALLKLCEVGAKEKEKEKQTEASEK"
     gene            17535..17609
                     /locus_tag="PLM79_10680"
     tRNA            17535..17609
                     /locus_tag="PLM79_10680"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17567..17569,aa:Asn,seq:gtt)
BASE COUNT         3611 a         5385 c         5193 g         3533 t
ORIGIN      
        1 ccgtgctttc caccatccgg tacttccggg acgagtacga agcccacatc ctcaacaagg
       61 agtgcccggc ccgggtatgt cgggacctca tccggttcaa ggtgaacgag gaaaagtgca
      121 ccaagtgcgg gagctgctac aaggcatgcc ccgtggacgc gatttcctgg gccaagaagc
      181 agcccgccgt catcgatact tccaagtgca tgaaatgccg gtcgtgcatc ctggcttgcc
      241 ggttccacgc catcgattga catagggaaa aacggccaag gcgatactga tttccgaatc
      301 tcaaacaagc gagagagagt ctatggataa ggacacgatc acccttacag tcaatgggaa
      361 agattttaag gggaagaaag tccaggggaa gaaggggcag accgtcctcg aggtccttcg
      421 ggccaacgac gtctacgtac ccaccctgtg ttttcacccc aagatgcccc cgtacggagg
      481 atgccgcttg tgcattgtgg agatcgacaa tatgcgcgga ttgcctccta cctgcaccac
      541 tcctgccgtc gacggtatgg tggtgaacac ccacaccccc aaagtggtcc gggtgcgaaa
      601 gacggtcctc gagctcctcg ttgcctacgg ggaccacaac tgcctgctgt gcgagcagac
      661 gggcaactgc gagctccaga acctcgtcta tgagcacggc atcgaccata tccgctataa
      721 gaccgacttc gtcccgaaac cccgggacga ttccaacccc atgatcattc gggaccacaa
      781 caagtgcgtc ctgtgcggcc gatgcgtccg cgcctgcctc caggtccaga tgaacggggt
      841 catcgacgtg gcggcccgcg gctccgacgc ctacatcacc acattcaaca ataagcctct
      901 catggaatcc tcgtgcgttt tctgcgggga gtgcgtccag gcctgtccgg tgggagccct
      961 taccgaaaag aaggcctatc gcctgggacg cccgtgggag agcaccaagg tccgcaccac
     1021 ctgtccctac tgcggagtgg gctgccagtt gtggctccac gtcaaagacg gccgaatcac
     1081 caaggtgacc ggtgtggaag acggagaacc caaccggggt cgcctgtgcg tcaagggccg
     1141 cttcggctac gacttcctct actccgagga aaggctccga actcccctca tccgggaaaa
     1201 cgggacgttt cgggaggctt cctgggacga agccctggga ctggtggctt ccaagttcaa
     1261 ggagatcatc cagaagcacg gccccgacgc catcgccggg gtgagctctt cccgaagcta
     1321 caatgaagac tcctaccaga tgcagaaact gcttcgttcg gtcatcggaa ccaacaacgt
     1381 ggacaactgc gcacgtactt gacacgcccc caccgtcgcc ggtctggcga aatctttcgg
     1441 ctccggggct atgacgaatt cctttgcgga cttcgccaag gcacaaatga tctttgccat
     1501 cggcaccaac atgaccgagg cccatcccgt ggcgtccacg ttcgtgaaga acgccgtgga
     1561 ggacggggcc cggctgatcg tggtggatcc tcgccgcacg ggcctcgccg attgggccca
     1621 gctccacgtg tccatcaagg tgggaagcga tatcgccttc ctcaacgcca tgatgaacgt
     1681 cctgatcacc gaggatctct acgacaagga atacgtggct tccaacaccg tgggattcga
     1741 ggaattgaaa gccaaggtca tggaataccc gcccgagaag gcggccccga tctgcggggt
     1801 cgacgccgat gtcatccggg acgtggcccg gcgcctggct tcggtcaaac ccgcccttct
     1861 tgcctacacc ctgggcatca ccgagcacac gtgcgggatg aacaacgttc tctcctgcgc
     1921 caacctacag atgcttctgg gcaacgtggg gttcgagggc ggcggcgtca accccatccg
     1981 gggtcagaac aacgtccagg gcgcctgcga catgggagcc cttcccaacg tgttccccgg
     2041 ctaccagcgg gtggacgatc ccaaggccca ggccaagttc aaggccgcat ggaaggtgga
     2101 ttccctgccc gaaaagcccg gcctcatgat gccccagatg atggaaggcc tcgccgacgg
     2161 ccgcatcaag ggcttctaca tcttcggcga gaacctggcc aacagcgaac cggatatccg
     2221 tcacgtggag cacctcctgg cctcggcgga attcctggtt tgccaggaca tcttccccac
     2281 ggaaaccact cgattcgccc atgtgatcct gcctgcagcc gcatggagcg agaacgaggg
     2341 gaccttcacc aacagcgagc ggcgggtgag tcgctgccgc aaggtgagtg aaccgccggg
     2401 aatttccatg cccaactggc ggattttcaa ggaactcgcc cggcacatgg gacatgaatg
     2461 ggaatccaac agcgcccagg aactctggga caacgagatc tcggttctgg cccccgctct
     2521 ggcgggcatc aagtatgctc gaatcgagaa caatggtctc cagtggcccg tgcccgccca
     2581 agaccacccg gggaccccgg tgcttcacaa gaacggtcag ttcacctccg ggaaaggtcg
     2641 gttcgtcgcc gtggactgga ctccccccgc cgaggtgccg gataaggagt acccgttggt
     2701 gttgagcacg ggacggcggc tcttccacta ccacacccgc acccagaccg gtcgagcggt
     2761 cgggctcaac gaaatcctgg gcgaggaaac ggcggacctg tcgccccagg atgccgaagc
     2821 cctgggaatc gtcgacgggg agatgatccg ggtgaaatcc cgccggggcg aagtgaaggt
     2881 caaggccaag gtaacggacc ggatgcccga agggatggtg tggatggcct tccatttccg
     2941 agaagcctgc gccaactggt tgaccaaccc ggccttcgac cccgtcaccc aaacggcgga
     3001 atacaaagcc tgtgcggtga agatcgagaa actgtagagt gaaacctcgg gaagggtccc
     3061 ctccgggact cttcccgttt ccattccgcc gccttctccc ccccgatttt cctccccggg
     3121 aaaacccatc gaacccacaa gcctgcctat tggatccccg gcgcacccac gcccacggac
     3181 ttgcccggag ggagccgtta ccagtttgca gaggactatt ggctcaaaat atccagcgct
     3241 tatcttgagc ggacggtcgt cgggagcacg gaggacatgt catttgcaaa ggactgtccg
     3301 aacgtgtatc ctgtattgta aaccatgcca ttgggtccgg atccgaaagg aaatccttcc
     3361 aagtaacggg gcgacaggaa aaggaaaccg aacggtggaa ccggcaaacg agcgcctgat
     3421 ctgtaatgtc aagtccatac ggaaggctcg agggctctcc cagacccaac tggccaagtt
     3481 ggtgggagtg cagcggcagg ccatttacga catggaatca gggcgctata tgcccaacac
     3541 aagcgtggcc ctgcgcctgg ccaggcacct gcaatgcaaa gtggaggacc tcttctccgt
     3601 ggaagaaccc gacctcgtcc aacccgtcac cctggtcggc ccggcggttc aaaccggtag
     3661 ccgcctccgc ctgggcaggg tccgcaaccg cctggtgggt ttccccctgg acgggaaaga
     3721 gatgctcaac gatgcgctcc tgagcgcaga cggcctcctg gacccccaaa gcgggcgcat
     3781 acgcctcttt cagagcgcgt cccgcctgga ccggacggcc ttgctcctgg gatgcgaccc
     3841 ggccttctcc atcctgagtg cccatgtgga gcgccaaacc cgcgaggcac gagtcgtctg
     3901 caggttttca tccagctttc gggccctgga aggcgtggcg tcgggaatcg cccatgtggg
     3961 gggaactcat ctccacaact cccggcaagg ggaagccaat ctcctcctcg cccaaagcgc
     4021 ccttgccgac accagggcga ccctgctggc cttttccttt ttcgaagagg ggctcatggt
     4081 cgctgcgggc aatcccctgg gaatcaagag cgtcggagat cttgccggaa aggatgtgcg
     4141 gtttgtgaac cgggaggaag gggcggccct gcggattctg ctggacgagc acctggcgag
     4201 ggcgggggtt cgcaccgaaa gcatccgcgg atacaaccac ctggtctcga accacaccca
     4261 gggagccctc atggtggtct acggcctggc cgacgcagcc ctggggcttc gcgccgttgc
     4321 cgcagcccac ggcctggatt tcgttcccat cgaggccgtt cggtgcgacc tggtggttcc
     4381 cgtcgatctc atggaccatc ccgccgtcgc attgatcctc gatgtgctcc aaacgcgctc
     4441 gtttcgcgaa gaactggcgt ccctgccggg gtacgaatct tcctgtaccg gcaaggtcgt
     4501 cggcaatacc tgaaagaacc tccgaccgcc ggtgccgctt ctttggcccg gcacgttcca
     4561 tcccacccgg acggtccccg gaaccccgca acgggaaatc tcctttcccg tgctccaccc
     4621 attccgggca cttcccccct tgaacccctc cggaaacgga agcgtttgct gcgctccccc
     4681 aaaaccgttg ccggattcgg aacggagagc aggtaccgtg gcgcggccgg cactcattga
     4741 gcacgttggg gaccgtccct cggtggaacc cgtccgtcgc tggaaggcat gggggcgaca
     4801 agcagctcga agagatggcg gctgagcctg gttgcggaca agttcccgag gccccgggac
     4861 ggatcccggc ccttcgagcg ggcatttatg ccgtcgaaaa tggacatccg gtttgcatta
     4921 gactgtgcgt caatgtatag tgtcgtctat atacgacgca tgtcgggatc tcgagaaagg
     4981 acgagccgat ccatgtccca gagcctcata ccgctgctcc tgaccttgaa ggtggcggga
     5041 ttcgccaccc tggcggcctt cctgatcggg gtctcctccg cctttctgtt gacccgggtg
     5101 cggtttcccg gcagggaatg ggccgacgcg atcctgaccc tgcccctggt catgccgcct
     5161 accgtgatgg gctatttcct gatcgtggtg ttcgggcgca aaggctggat cgggcattgg
     5221 ctctacgacg ccctgggcat cagtctcatg ttcacctggc agggggcggt acttgcttcg
     5281 tccgtcgttt ccctgcccct ggttttcaag gccgcacgca gcgcttttga aggggtcgac
     5341 tccaacctgg aacgggcggc ccggaccctg ggcctgtccg agaccgcggt gtttttccgg
     5401 gtgtctttgc ccctggcctg gcgcgggatc ctggcgggaa ccatgctggc ctttgcccgg
     5461 gctatggggg agttcggcgc caccctcatg gtggcgggca acctgcccgg caagacccag
     5521 accctctccc tggccgtgta cgaagcggtc caggcaggca atgaccgact ggccgccacc
     5581 cttgtgctca ttacctcgtt ggtttgcgtg gtgattctcg tctcatcggg gaagattctc
     5641 aaaacccaat atctcaagga ggttgatcga tgaaggaaaa gctactggtt gttatcgcgg
     5701 cggccttggc ccttctcggc atgcccccga taagcccggc gaaagggatg gcggaacagg
     5761 aactcatcgt ttccgcggcg gcgagcctca ccaatgcctt ccaggaaatc gccgggaaat
     5821 tcgccgccgc aaacccggga acgaaggtga tccccaactt cgccgcctcg ggggccctgt
     5881 tgcagcagat ggaaaaggga gcgcccgtgg acgttttcgc ctcggcggac cagaaaacca
     5941 tgaaccaggc ccaagaaaag aggctgatct ttcccgagac ccggaagaac tttgtgagca
     6001 acggcctggt gctgatcctt cccgtgggtt ccaaaacccc cgtccagggc tcgcgggatc
     6061 tcctggccaa agaggtcacc agggtgtccc tgggaaatcc cgagacggtt cccgcgggcc
     6121 gatacgcccg ggaagccttg gagaatgaag gtttatggga aaagttgggt tccaagctca
     6181 tcccggggga ccatgtccgc caggtgctgg actacgtcgc ccgaggcgag gtggacgccg
     6241 gatttgtctt tgccaccgat gcggcccagg ccaaggacaa ggtgcgggtc gttgcccttc
     6301 tccaacagca tcagcccatc ctctacccca tcgccgtggc ggcggccacc gggaagaagg
     6361 accttgccca acgcttcgtt gatttcgtgc tcaaccggga aggacaggag atcctttcca
     6421 ggtacgggtt tgggaaaccc tgacatggtc atacgcgtgg aggtggaaaa aaggatgagc
     6481 tcccgggccg ggcaattcga gctccgtgtg gccttttcct cccgggaccg gtgtgtcgtc
     6541 cttttcggcc cgtccggttc cgggaagacg ctcaccctgc aatccatcgc gggcctggtg
     6601 acgcccgatg caggaaaaat cgccctcaac ggacaggtgc tcttcgattc ccaaaacggc
     6661 atcaatgtcc cttcgcgcca ccggaatatc ggctacgtct tccaggacta cgccctcttc
     6721 ccccacctca ccgtgatgga caatgtgggg tttggcctga gaagaacatg gcaccggggc
     6781 ctgtcccggg aagaccggcg ccaaatcatg gaattcctcg gaatttttga gatctctcag
     6841 cttgccggga gttttcccgc cgacatttcc ggaggccaga agcagcgcgt ggccctggcc
     6901 cgagccctga tccgcaaacc ggacctgctg ctcctggatg aacctttttc cgccctcgat
     6961 accctcctgc gcaggaggct ccgggaagag ctggtaaaga ttcggcacac cttcgacctc
     7021 cccatggtca tggtcaccca cgaccccgag gatattcgag tcttcgccga gaccctggtg
     7081 gtttacgaag cgggccgggt tcgagagagc cggcattttc tcatggacaa cgggatcgac
     7141 ctggaggaag tgcttcttgc gtgaccgtcc cgtcgaacga gctcgtctcc cctgcattca
     7201 agtctgtgcg cggcggaatg tgctccggca cactgggccg caggtggatt tcggggcgat
     7261 tccggacgcc gccgcccaac tcgaaaggtc tgtccaccga ttgtcccgat taggtcgaca
     7321 gggcggtggg gaaatgcagc agtgtgaatg tgaaccccct catccagggg aaaagcatgc
     7381 aactgaacac ccggaatatc ctgaaaggca aggcggtcga tctgaaggaa ggccgggtaa
     7441 tggcgaaagt gaaggtcgac tcggtggtgg gaacctcatc ccctcgatcg tcaccgtgga
     7501 ggccgctcaa gatctgggcg gcaaggtggg cgatgaagtc cgcgtcctga tcaaggcgac
     7561 cagcatcatg atcggaaagg ggtagccggc ggcggagggg gaagaaacta tcccgctgct
     7621 tcacccaaag cccatggggc ggggttttcg ccccgcccca cgtcgtgcct catccggtgg
     7681 tttcccgggg cggcttccgg ccggaaggcc cgcaccccaa ggaagtcccg ccgggaggat
     7741 tctcgaatat ccaagaaccg aatcggcccc ccgggggaaa accccgccgg ggtgcctgga
     7801 ggacctgcct gcggacctac cctgcagcgt acttgtgata cttcttgggc tcatccgtca
     7861 ccacgaagat cacccggact tcgtcggctt tgagggcctc ggccttggga tgggtcttct
     7921 tgagttcttc tacccgtttc tttgccaggt ccaagatctg ggaacgctcg ccgaagatca
     7981 tggctcccgt gggacaggac ttcacgcatg ccgggggccg gtcgtgggtg atgcgatcga
     8041 tgcacatggt gcatttggcc aggaccttgg tcttttcatt ctgcctcggg acatcgaacg
     8101 ggcaggaact gcgcacttca tcgaagggcg cttccttgga tttgtccgtg taggtgacgg
     8161 caccggttgc ttcgtctttc acggctccgc catccacgta cccctgaatg gcgtccacgc
     8221 aggggggatc gaggcaatgg cgacacatgt ccgagaagaa gtaccagtaa ggtttgccgt
     8281 tttccaattt accgtccgcg aagcgaacca ccttgtaggt atcggccgag aagtcctgcg
     8341 ggttctggta gttgccgagc tgctcggtct tggtacccgg cagaccgttc cattgtttgc
     8401 aggcaacctg gcatccccgg caggcggtac acttggaggt atccaccaat atggattttc
     8461 cgctagccat tgatcaaccc tcctatgcct tggtcacgtt gaccatgaac gctttgtatt
     8521 ccggggtcat ggtgtttccg tcccctacgg tgggggtgag caggttggcg ctgtcgccgc
     8581 agtccttggg gaaaagccac ccgtaattga acgtcattcc cacctggtgg atcacctgtc
     8641 cgtccacctt gaacgggcgg aagcggggag tgaccatggc cacgcattcc agggagccgc
     8701 gtgccgattc caccttcacc ttgtccccat ttttgatacc ccgaagatgg gccagctcga
     8761 ggctcatttc cacgtacgcc tggggctgcg cttccgtgag ccaggactgc caccgggtga
     8821 tgcctcccgt gcaccagtgt tcggtgcacg tgtaggtgga catgacgaag gggtacttgg
     8881 gattgcagct cgcgtacacg tcgcagtcgc ccttgaacag tttgatgatg gggctgttga
     8941 gctgggacga catgaggttc ttgggcagcg gactctccac cggttcgtag tgctcgggga
     9001 agggaccgtc gcccaagccg ggaccgaaga gggacccaac tccgtcgggc ttcatgataa
     9061 aggggtattt tcccttctcg gcgtcggcca tgggaggcca cggaccatcg ggaacgtccc
     9121 ccacccactt gccgtccttc caggccaaca ccgctcgctg gggattgtac gggcggcctt
     9181 cgagatccac cgaggcccgg ttgtaaatga tgcggcggtt gacgggccag gcccaggacc
     9241 attcgggata gagtcccagg cccgtgggat ccgtcttctt ccttcgagcc atgaggttgg
     9301 tgccgtcctg ggcgaagctt ccgcaagaga tccagcagcc gctcgaggtc ttcccgtcgg
     9361 cttggagcaa ggcgaaggac gggacacagt cgcccttctt gtagaccttg tctcccacgg
     9421 tgacgtcttc caggaaatac ccgttgacgg ctttggcaac cttgagggcg ctgaagttgc
     9481 ctttctcatc ggcgtaatcc catttcaggt tcaggatggg ctcggggaac ctccccccct
     9541 tttgcttctt gtagagctct tttaccttgg ccatgatgcg atagacgatg tccccggtgg
     9601 agatggcgtc ccccggagga tccgccgccc ggtatttcca ctgaacccat cggccgctgt
     9661 tcgtctggct tccttccttt tccaccgaag cggaagcggg aaggaggaac acctccgttt
     9721 tgatcttttt gggatccatg ccgggtccct tccagaagga ggccgtctcg ttgtcaaaaa
     9781 tgttcacatg gacgaaccag tcgaggttcg ccatggcctt gcgcaccttg ttggagttgg
     9841 ggaggctgca agcgggattc tgggcgatgc acatgaaccc tttgatcttt ttttcataca
     9901 tttggtcaaa gaggtacatg accgaagcat cctgtccgtc atccaacttg ggcacccact
     9961 catacccgaa atcgttctcc ttggcagcct tatccccgta ccagctcttg aggaggctta
    10021 cggcgtactt gggatagttc tgccaccagt tggccgatcg aggatccgtg gacttggggg
    10081 tgttcttctt gaggtaatcc tcgagggtta cctgggaggc ccggggagcc gccaggtagc
    10141 cggggaggta atggtacagg atggcaaaat cggtggaacc ctgcacgttg ggctccccgc
    10201 gcagggcatt gaccccgccg ccgcagatgc ccatgttgcc caggagcagc tgcaccatgc
    10261 acatggtgcg gatattttgc accccggtgg tgtgatgggt ctggcccagg gcgtacatga
    10321 tggtgcccgc cttgtctttg acccccgtcg aagaatacag ttcatagacg gtcttgaggt
    10381 cttccacggg ggttcccgta atggaagata ccttttcgag gtcgtaacgg gaataatgct
    10441 tcttcagcaa ctggaagaca cagcggggat tctgcagggt catatccctt tcgggaatcc
    10501 cgccggcatc ccgtttgatg gcccaggaca ccttgtcgta cttgcgggtg ttgggatcgt
    10561 agccggaaaa gaggccgtcg tcgaagccga atttgtcatc caccaggaag gaagcgttgg
    10621 tgtagttttt cacatattct tcgaaatatc tatggttgtc caggatgtac ttgatcattc
    10681 ctcccaggaa ggcgatgtcg gtgcccgagc gcaaagccgc atacacgtcg gctcgagcgg
    10741 aggtcctggt gaagcggggg tccacgtgaa tgaccttggc gcccctttcc tgcgccttgg
    10801 tgacccactt gaaggagatg ggatggtttt ccgccgcgtt gctcccctgg atgaggaaga
    10861 cgtccgcatg ttggaggtca atgaaatgat tggtcatggc gccgcgtccg aacgactctg
    10921 ccagagccgg tacggtgggc ccgtgtcaga cccgggcctg gtgatcgata tggatcagtc
    10981 ccagggcccg caccatggcc gtgatggccc aacattcctc gttgtccaca ttggagcttc
    11041 ccatgtgggc aatggcttcc acgcggttga ccacctgccc cttgctgttc ttgacgataa
    11101 aatgagcatc ccgggtcttc ttgatcttct tggcgatttc cccaatggcc cagtcccagg
    11161 acttcacctg ccacttgtcg cttcccggag cccggtaaag gaccttggtg aggcggtgct
    11221 cgttggcggc cgtagtctgg aagatcccgg cccccttggc gcacaaggcc ccttcactga
    11281 tggggtgttc cgggtcccct tcgatgttga ggatcttgtt ggttttggtg tccgtgctgc
    11341 aaagaagacc acatcccacc gagcaatagc agcagatgga cgtgctttcc ttggcagtct
    11401 tcagcttgtc cactttcctg atctcctggg cataagcgcg gatgggcccc aaatccatcc
    11461 cgagggatga cagagccagt cctgcgccca tggcaccgga gaccgtcaga aactcccggc
    11521 gcgtaattcc catgctgtcc ctcctaacta aggcctgaat tccctccatt ccacattttc
    11581 ggtcatgctc ttgacgaaaa ccccgattcc gggggacatg cggaccgccg agccctcgaa
    11641 aagcaggaga cggtttcctt ccccttggcg ggcacccacg gcttcccgag gaaagcccat
    11701 gggcccgcag ttcctatgag ccgcgcattc acagatcgca ggggatgaga aacagaacgc
    11761 ttttcgaaaa tccgcaaccg atgatgactt ggggaatagc ccatttgtcc cagtatttca
    11821 atgtgttaag ataagcacat accctgcggg atcggactac caggcacgat gggggtgggc
    11881 gacggttcaa ctcccatagc gctccacgcg acagtcgaag atttctccgg ccttacgaag
    11941 agcatcgtcc tccacttcct ggaaccagcg gaggaatttt tccgtgatcc gcaggccctc
    12001 ctccgtcaac tggcacccct ccttgcggca cccgttggac gcaatgagcc gaatccccag
    12061 gatctcttcg ctcttgcgga tcttgcccca ggcggctcga taggacatgc ccatctgatc
    12121 ggccgctttc tttagggacc ccaattcctg caccttgagg agcaacatgg cccgccccat
    12181 gccgaagtac acgccttcgc cgttttcgag ccaaagatgg agccgaaccg tcggagaggg
    12241 tcgtttcata caatctcccg gtcaggatgg gactgaagtt gccgattcca tcggcggagt
    12301 tgtcgcacgc tatgtatgac atgacatatg gcgtaggcaa gtatatatgg gggaatgtcc
    12361 ctgtcaaggg aaaccggccc cgtactcgga ggatccgcgc cctcaaaatc ggaatggtcg
    12421 aaagaggcac aaatatcctg aaaataagtg aattataccg attttaggtc tttaaggaag
    12481 gtgatgtcgg gattttgcgt tatgctgtac ttgacacata gtagggattg tggcacaagg
    12541 caatcgtcag cttggcaaca ctgtccaacc tcattgggag tcgggccccc aggggcccga
    12601 tttcttctta aggttggagc ttcaggagtg gagcttcttt agcagcagag ccatgttgac
    12661 tcccaggagc ctcatggtct ccatccccac ctcgtccttc tccacatccc ctttctccag
    12721 gcccaatgcc atgttggggt agttggatcc ggccagaaac atttcaaagc agtaaaaaaa
    12781 gcggttcatg gcgtccaagg tgtggcaggc ccccagacga ttggctgccg tcagcgaagc
    12841 ccccaccttg tgtcggaaca tccccgggtt ggcaaaggcc accagaccga cccggtcgat
    12901 gagggccttc atccgcgggg cgacgttggc aaaataggtg ggagaaccca gcagaatgcc
    12961 ctccgcttcc accatcttgg cgatgcactc gttcaggtcg tcttctttgt tcgaacactc
    13021 ccgatcctgg ttttcaaaac accggtaaca ggacctgcag tcttcaatcc tcttttccga
    13081 aagctggatc agttccgttc cgatcccttc cccatcgagc ctttcgagaa cgcaccgaag
    13141 gaggatggtc gtgttcccgt ccttgcgagg actcccgttg aacgccacaa ctttcatcgc
    13201 aggcttccct tctcctgtgg gcacgctttc catcagcatc ggctttccac gtcgccccgc
    13261 agcctacacc aggaaccgcc gggatgcaat gcaccgacag ggtctttggc cgtttcggcc
    13321 tcgtgcggca ccccgagcgg atccgccgcg ggaaaaggat cattgaacct gccgggaggg
    13381 tcccgcccac gggtcggcag tcgggacgac gcccggggca aaactgtgga atgcaggaat
    13441 aggaggcgac aaaggcctcc cttcgcgaag caatccttgg atgtcacaaa cgcatggccg
    13501 aaggcgaatt ccgtacggat ccgggcacgc cgaagtcgac gcttccctat ttcgggattc
    13561 tcattcaatc cggaaggccc acggcaccat cgggccggaa gcaggccttc tcgagatcgt
    13621 cccttagccg tggggagggg cccacagggc catttccatg ggaacgtccg aagagatcca
    13681 tcggcagcgg gcttccatct ccgccagccg cccccggatc tcttccaccc gcaggcgaag
    13741 attctccccg tcgggatgat tccgcaactg cctttccgcc tcctgcaacg cacgcatccc
    13801 ttcgaggtac tccctttcgc attgcagagc ctcttccctg agcctcaggt actctgcgct
    13861 ctcctccatt tcgtcaaaaa accacatgga catgggctcc tccttccggg caagagcaaa
    13921 accctttcaa gattcggaac cggctgaaaa aaaagggctt tccctccacg cagtacggag
    13981 ccctttcccg cccgtcgacg atagggacga gaaccgtccc tgccggacac ttcttcatca
    14041 gaataaggaa aatcgaatcc gaatcaagat cggacaacgc ccaaggaccg gtccccggga
    14101 aaagggatcc ggagccgctt ccgacatccc gccccccggg gtccaagggc ctggtatgct
    14161 caccggttct gagcgaagtc gggctgaaga tccccttgct ttgggcgaaa aagatgcaca
    14221 agcggcgaaa atattcttga cctttttttt tgattgttgc tagatatcaa aaaaagttta
    14281 ctaatgtgag acaaaccctc aaccggaaac agtaacaaga gttctctgtc ggaatgctcg
    14341 agctggaggg gtgcaaggcc tctcggacgt gagcgcggtg gcccgtaacg aagaaattgg
    14401 agtgaagaca tgattgagat tcgttttcat ggtcgaggag gtcagggagc agtcacatcg
    14461 gcggaactca ccgccctggc cgcaattgaa tcggggaagc atgcccaagc tttcccaagt
    14521 tttggcccgg agcgtcgcgg tgcgccggtc atggcgttcg ttcgggtgag cgatacgccc
    14581 atctccaccc gcgaaaaggt gtacgagccg aacattgtgg tcgttctgga ccccaccctt
    14641 ctgaagctgg tgaatgtgga agccgggctc agaaaagggg gcatggtgat cgtgaacacc
    14701 accaagagtg ccgaggaact gcgtaaggaa acggggatca aggccaaact tgcggtggtg
    14761 gatgccagca agatcgccgt ggagaccatg cgggttccca tcaccaacac caccatgttg
    14821 ggggcattgg tcaaggcggc gcctgaggtg atttccatgg aatcactggc aggtcccatc
    14881 gatcatcgtt tcggccccat tgcggcaaaa aacatgaatt cgtgcaagcg cgccttcgat
    14941 gagacgaaag tggagggtta agctgtggct aaagagaccg gaatcatcag ctggaaagag
    15001 ttgaacctgg ggattgccat caccacgccg ggcaacgcgg ccgagctcaa gacgggcgac
    15061 tgggcagctc aacgtccggt aaccgaccgc gagaagtgca ccaagtgcgg acagtgctac
    15121 atcttttgcc cggacatggt gtacagcaaa gacgccgagg gctattacgt tcaaaactac
    15181 tatcactgca agggttgcgg aatttgcgcc aaagaatgcc ctgtagatgc gatcaccatg
    15241 gtacaggagg gcgattaata tgtcgaaaca cgttggaatg gaagtctcca ttgcagctgc
    15301 cgaagcggta ggcctctgtg atgtggatgt ggtagccgcg taccccatca cgccccagac
    15361 tcacatcgtg gagcacctct ccgagatggt ggctgatggt catctggacg ccgaatttgt
    15421 ccccgtggaa tccgagcaca gcgccatgag ctgctgtatc gggtccagcg ccgccggtgc
    15481 ccgcaccttc acatccacct gttcccaggg atacgccttg atgtcggagc tttgctacat
    15541 cgcggcgtcc ctccgtctgc ccatcgtcat ggccgtggcc aaccgggcct tgagcgcccc
    15601 catcagcatc tggaacgacc atgccgatct catgtacgct cgagacaccg ggtggattca
    15661 gctcatcggc gaattcggcc aggaagtggt ggacctcatg cccatcgctt tccggattgc
    15721 cgaggacaag agggtccttc tgccggtgca ggtcaacctg gacggcttca ccctgagcca
    15781 cgtgatcgag cccttcttga tgccggacaa ggacgaggtg aagaaattcc tgcctccctt
    15841 caagccccag ctccgcctcg acccggccaa tcccgtgacc tttggtcccg tgggcatgcc
    15901 ggacgtctac accgaagccc gcaaggccca ggatgaggcc ctcaagaact ccaagccggt
    15961 catccaggaa atcttcgacg aatggggcaa gcagttcggt cgcaaataca agctgatcga
    16021 aacctacaag agcgaggacg ccgacaccat catcgtcacc atgggcagca tcgcccaaac
    16081 ggccatgacc gccatcgacc acctgaggtc ccagggcaag aaggtcggcc tggtccgttt
    16141 tcgcgtcttc cgtcccttcc ctgcaaagga actcttcgac gccatcaagg gcgccaagaa
    16201 cctggtgctt ctcgaccgag tcagtgcgtt tgcgggtgga gaaatttccg ctccggtggc
    16261 cctggaaatc aagtccgtcc tgtacgaccg gaagcattac cccaaggtgg gcaacttcct
    16321 ggtgggcctc ggaggtcgcg atgtgacggt acaaaacttc gaggaaatgg tggagagaat
    16381 tgccgccgcc aaagacggtg agcttggagc ctacgaattc atcaatgtga gggaataaat
    16441 gggaatcgca tctgaagctc tgaagaattt caaggggttt aacctcaaga acttgcccga
    16501 ggtggaaccc gtatcgcccg gtcatcgcgc gtgccaggga tgcggagagg tcctggcttt
    16561 gcggcaggca atgaaggcaa tcggaaccaa cgtcgtcgtt tgcagcgcta ccgggtgtat
    16621 ggaaatcatc acctctccct ttccccagac agcctggcga gtgccctgga ttcacgtggc
    16681 gtttgagaac gccgcagcag tagccagtgg tgtggaagcc gcctacaaag ccatgaaccg
    16741 caagggaatc atcaatcagc ccgataccgt gttcgtcggc tacggcggag acggcgccac
    16801 ggccgatatc ggcatgcagg ccctctccgg cgccctggaa cgaggccata atttcatcta
    16861 catgtgcttc gacaacgaag cctacatgaa cacggggatc cagaggtcct cttcaacccc
    16921 ctacggcgcc gggaccacca cgtcgcctcc gggcaagaag agcatcggcc agtccacgtg
    16981 gaagaagaac atgtccgcca tcgcggtggc ccacggggtt ccctacgtgg caacggcctc
    17041 tccggcctac cccatagacc tcatgaacaa ggccaagaag gccgccatgg ttcccggccc
    17101 tgcctatctt cacatctact cgccctgccc cacggggtgg cgctgtggaa tcgaggacgg
    17161 catcgaagtg gcgaaactgg ccgtgcagag caaagtcttc ccgctctacg aggtcatcga
    17221 cggggtatat tacctgtccc gcaaagtggc caaacccaag ccggtagccg agtatttcaa
    17281 accccaacgt cgcttccgcc acctgaccga ggccgaggtc gccaagatcc aggctcgggt
    17341 ggaccaggaa tacgaagcgc tcctcaagct ctgcgaagtg ggagccaagg agaaggaaaa
    17401 ggaaaagcag acggaagctt ccgaaaaata acggcatcgc gacgggaacc aagtcgctga
    17461 ttgatttccg acgcaccttt tgattgacaa acgaggggtc ctctgttaca gaggtctcct
    17521 cgtttttcgt ttgatccccg gtagctcaat ggcagagcgg gtggctgtta accactaggt
    17581 tggcggttcg agtccgtccc ggggagccaa caacgaatgc aacccgcttc ataacgggtt
    17641 gtttaactgt ttgccgcccc gcgaattcgc ggggcttttt tttgtaagcc ctgaacttcc
    17701 cgggagttac ggaggctgct gg
//
LOCUS       DAOOVC010000076        17588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2210_length_17588_cov_7.487766, whole genome shotgun sequence.
ACCESSION   DAOOVC010000076 DAOOVC010000000
VERSION     DAOOVC010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17588
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2210_length_17588_cov_7.487766"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..557)
                     /gene="cas2"
                     /locus_tag="PLM79_10685"
     CDS             complement(267..557)
                     /gene="cas2"
                     /locus_tag="PLM79_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOV86819.1"
                     /translation="MMVVVSYDVSTMDSEGQGRLRRVAKACKDYGQRVQFSVFECSLD
                     PAQWVRLRDRLLKLIDPEKDSLRFYFLGANWRRRVEHVGAKKVVDPEEPLVV"
     gene            complement(566..1597)
                     /gene="cas1c"
                     /locus_tag="PLM79_10690"
     CDS             complement(566..1597)
                     /gene="cas1c"
                     /locus_tag="PLM79_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOV86820.1"
                     /translation="MRKHLNTLFVTTQGAYLSKEGETVAVKVDGEVRLRLPVHTLGGI
                     VCFGNVLCSPFLLGFCAENDVTISFLTEHGRFLARVLGPVSGNVLLRRIQYRKADDPQ
                     SSAEIAASVLTGKLANCRTVLRRVLRDHEDKVNAPAIQDAASRLTQCLERLGFQTTLD
                     ALRGIEGEAAHGYFSVFRELILAQKEDFTFQERNRRPPLDNVNSLLSFLYTLLMHDVR
                     SALECVGLDPAVGFLHRDRPGRPGLALDVMEEFRPFLADRLALSLINLKKVQGGGFKK
                     TDTGAVIMSDETRKEVIVAYQNRKQEEILHPFLGEKVRVGMLFHIQALLLARSLRGEL
                     DAYPPFIWK"
     gene            complement(1594..2229)
                     /gene="cas4"
                     /locus_tag="PLM79_10695"
     CDS             complement(1594..2229)
                     /gene="cas4"
                     /locus_tag="PLM79_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOV86821.1"
                     /translation="MKYAEDDLLPLSALQHLVFCRRQCALIHLEQVWDENLFTAEGRV
                     MHERVHQVGEESRGSVRIVRGLPLRSLRLGLVGKADVVEFHRVEKNVWRPFPVEHKRG
                     KPKPDESDKVQLCAQGLCLEEMLGIDVPTGSVFYGRTRHRLDVSFDSDLRGETERVAR
                     ELHALIESGNTPPPVYTKKCRTCSLMDRCLPRLLDGKRSVRAYLSKAVEEG"
     gene            complement(2570..2761)
                     /locus_tag="PLM79_10700"
     CDS             complement(2570..2761)
                     /locus_tag="PLM79_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86822.1"
                     /translation="MVTRTYKNMQLKLVRPVRASPKVLLQAYRDFLRERGLPLREKDY
                     HEAGPVPGGGGRDKSYPSP"
     gene            complement(2783..2959)
                     /locus_tag="PLM79_10705"
     CDS             complement(2783..2959)
                     /locus_tag="PLM79_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86823.1"
                     /translation="MQEPENRQKGGAKKIIPPHGGYRDLVFCPMVEIVHDATVVFCNR
                     SRTHEQVVRVVQIG"
     gene            complement(3100..3966)
                     /gene="cas7c"
                     /locus_tag="PLM79_10710"
     CDS             complement(3100..3966)
                     /gene="cas7c"
                     /locus_tag="PLM79_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOV86824.1"
                     /translation="MKRYDFVYLFDVKDANPNGDPDAGNLPRIDPETGEGLVTDVCLK
                     RKIRNYVGLKHQNSPPHEIYFTEGAVLNATHRRAYEAVGEKPEPKKLPKDVAKAKEVT
                     DFMCRNFYDIRTFGAVMSTEINCGQVRGPVQLSFARSIHPIVALEHAITRSSVTNEAD
                     KEKERTMGRKYTVPYALYRAHGFVNPFLAEPPKGTGFGDDDLDLLWESLMNAFQFDQS
                     AARPAGSMAARKLVIFEHESPLGSVPSHELFDRIRIKRKDDSGPARDYGDYVLEIDDE
                     NLPLGVRLITKP"
     gene            complement(3963..5792)
                     /gene="cas8c"
                     /locus_tag="PLM79_10715"
     CDS             complement(3963..5792)
                     /gene="cas8c"
                     /locus_tag="PLM79_10715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOV86825.1"
                     /translation="MILQSLYDLYDRLVRDPEYGISQPGYSLQRISFRLVIKNDGTLH
                     GIEDLRDGNTGKPRLMIVPGGDKPTGKVTEHSVHRKVQFLRNDLPFLVGAMAGNDKEA
                     GLSPATREFEAFKKYHLEVESEISDPDYGAFCNFLRTWRPEEALVHEDWREFAAGQGV
                     IQLLGKTEYLHERPRIRAWWEGKQEAKTKDGVYGQCLITCQFKPIARLHEPKIKGVRG
                     SQTSGAPIVAFDKDKYAFASYGRDGEQGFNAPISQDAAFRYTTALNALLVGSKSDKHR
                     FSLADTTVVFWTKEPSVTEDIFARFALHGSMGTGQEEVQDQSLRQKIEFFFKALREGR
                     EAYSEIDAAPDRTPFFILGLTGQAKGRLGLRFFHRNTVSDLLDNLRRHFADMEISREY
                     GEDTRHPDPEFPAFWQLLDETCPRRNGRADRDKIPPILAGPLLRAVITGSQYPVGLFT
                     AVMRRVHTERMVNYIRASIIKGYLTRNQGKEVSMGLDVNRKDAAYRLGRLFAALEKTQ
                     GDALGTRNATIRDRFYSAASATPGAVFPRLLRTYQHHLAKMEKGKVGREILVREILEP
                     LTDFPSHLNLSDQGLFALGYYHQMRDLWTSKQDREKDEEGEES"
     gene            complement(5797..6513)
                     /gene="cas5c"
                     /locus_tag="PLM79_10720"
     CDS             complement(5797..6513)
                     /gene="cas5c"
                     /locus_tag="PLM79_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOV86826.1"
                     /translation="MAYGVKLLVWGEFASFNRPEMKVERVSYEVITPSAARGILEAIY
                     WKPEMRWVVDRLRVLNPIRFTHIRRNEIDCKISVRSVVSGLKAGCCSLGIAVEDHREQ
                     RAAMILKDVCYGIEAHVEVVKPELKGGKPIEAPEAKHLRQFKERARKGACYHHPYLGT
                     REFPADFSLVEEFPACHESLRGERDLGYMLHDIVFTPNPKGRIVETNQGRRLQAQPRF
                     FPAKMQDGAIEVPPLVSEEA"
     gene            complement(6529..8754)
                     /gene="cas3"
                     /locus_tag="PLM79_10725"
     CDS             complement(6529..8754)
                     /gene="cas3"
                     /locus_tag="PLM79_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOV86827.1"
                     /translation="MPCQSHYAHSLAERPPSEWQPLEEHLRNVAERAGQFAASFGACE
                     WGIHAGWLHDVGKYSLAFQEYLKTQNNVDASIENAPGRVDHSSAGAQYAARMPNLLGH
                     LLAYVIAGHHSGLLDGRSTGSCQEKRLEKSLSPWEHGLKVLPELPKLEGPDFLRIALS
                     RKDAFSVAFFVRMVFSCLVDADFLDTERFMNPDQAAWRKGWPNTVLKHMEDALTDYIG
                     RLDPRGNPVDRQRTLVRNACLHAAERQPGLFSLSVPTGGGKTLASLAFALRHAIINGL
                     QRVVYVVPLMSIIEQNAQVFRRAMKPLADGLGRDPVVEHHSNLDPDNETTMSRLAAEN
                     WDAPLVVTTSVQFYESLFANRTSRCRKLHRLARSVIILDEAQTLPVEYLKPCLRALQE
                     LTDNYRATVLLCTATQPEIQRTKDFEIGLRKPHEIIPDPVRLYRELERVRVENLGGQK
                     DEELSNRLMTEPRVLCIVNTRSHAARLFQAIGSAEGHFHLSGNMCAAHRSEKLERLRK
                     NLEENRVCRVISTQVIEAGVDVDFPVVYRALAGIDSIAQAAGRCNRNGKLPGVGRTYI
                     FRSEHTKSERFIADTIQCAGQILSGHDNPLDLKAVEHYFKLYYWDQRDRWDEKKIMDE
                     FSLHNDRSFPFNLGFGRSAGKFRLIDEAAYRPVIIPWGEEGRRLCERLRAMPAPTTEV
                     LRRLQPFLVQVPPGLWQKHVGTNIQLIHDGIAVLVSPELHYSDDIGLVLDAREPAGVF
                     V"
     gene            complement(9233..9796)
                     /locus_tag="PLM79_10730"
     CDS             complement(9233..9796)
                     /locus_tag="PLM79_10730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOV86828.1"
                     /translation="MADPAKKKAAYQDLFSIPENMTGEILGGELIITPRPSARHALAA
                     SVLGGRLLPPFQFGEGGGPGGWVILHEPEVLLGEDLPVPDLAGWRKERFPGMPAENWF
                     SVAPDWVCEILSPGTVGKDRARKMPVYARHAVKHLWIIDPAVKTLEVFRLESGKWLLI
                     ETVAENARVRAEPFVELELDLDSLWWD"
     gene            complement(10073..11839)
                     /locus_tag="PLM79_10735"
     CDS             complement(10073..11839)
                     /locus_tag="PLM79_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOV86829.1"
                     /translation="MHGFYGRILAVDLSRETSTVEPVPEEVLRECLGGKGLATRLLME
                     RNPPGADPFSAENHLIIATGPCCQSPVWGASRYGVYTKSPLTRLYVESYSGGKVPEAV
                     DSTGFDAVVFTGRAARPTIVSVDPRGARFHEAGDLWGADTFRTEAEARARFAPNKAGY
                     RKPGALVIGPAGEKLLRFALIENDKWRSAGRGGVGAVMGSKQLKAVVFQGDRRREPAQ
                     PGALAQYGKAFSKTARDNAGVKAYRALGTTMMVKILNEAQAFPSKYWTEGSCEHWEKI
                     SGQTFHREHQVRPHACAGCLMACGRLATIQKGRRKGLVIEGPEYETIYAFGGLCMIEE
                     IEEIAYLNDLCDRMGMDTITAGNLCAFTIEAVRRGKVRYEIDYGQPDQIARLLVQIAN
                     REGIGDVLAEGIAHAARQWDLEDLAVHVKGMEPAGYDPRTLKGMGLAYGTSDRGACHL
                     RTTFYKPELAGLIPRDAVEGKARMLIDYEDRLTIFDTLILCRFYRDLYPWEELEKVVG
                     MVTGLPSSREDLGKIASNIADMTRRFNLREGLDPEQDRLPRRLHREALPTGQALTAGE
                     METMLRDYYRIRGWSEDGVPRD"
     gene            complement(11959..13200)
                     /locus_tag="PLM79_10740"
     CDS             complement(11959..13200)
                     /locus_tag="PLM79_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOV86830.1"
                     /translation="MEQTPLLFTPIRIKGLYLKNRITRAPMYVGYGNPDGTVSELLLE
                     HYRLLAASGVSMVVVENAAVDFLGLGAPYMLRVDHDRYIPGLAKLAETIRAQGAYALL
                     QINHAGRFAFTAERLAPSSLQIGDVVPREMSEADIDRTVDAFASAARRVKEAGFDGVE
                     IHGGAGYLLAQFLSPRTNRRSDHYGGSLENRMRFPLRVVDAVLDAVGKDYCVGSRFMA
                     DEWLPELRLSFSLFKMLKRKYRFLSDELPGQALRTDETSIYARELEKKEIAYLSVMAG
                     TLDCFFLLQYLEQEKKEAYMTHFAEAIKKAVPNTPIITAGRIQSPQTGEEILQKGKAD
                     LIGLARVLFADPLWPQKAAGIIQDPIRRCEPSCSVCTQRALYGRPAFCSQWDFDRRDQ
                     FLARVSEMEAFLTKLEERDEI"
     gene            complement(13469..13714)
                     /locus_tag="PLM79_10745"
     CDS             complement(13469..13714)
                     /locus_tag="PLM79_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86831.1"
                     /translation="MASFYRFCMILGLLFLAAAAPMERSESRQGQALTLEPRNTNEMG
                     AVVGPSYTTPGAYGAHDARTGDRTSPYPPYFHPDQGR"
     gene            14009..14281
                     /locus_tag="PLM79_10750"
     CDS             14009..14281
                     /locus_tag="PLM79_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86832.1"
                     /translation="MRIDEEKGGICPLNPREAAEVFELGKRPWIPGVGRDRTMDKQEK
                     TLKGFTVAKRCDICGYRTKITSDTGDRCTLKCVHCGKEYSFYQRRP"
     gene            14522..14830
                     /locus_tag="PLM79_10755"
     CDS             14522..14830
                     /locus_tag="PLM79_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOV86833.1"
                     /translation="MFVVHVFVHVKPDRVEDFKAASLENAARSVLEPGIARFDVIQQI
                     EDPTRFVLVEVYRTPDDPAKHKETAHYRQWRERVEDMMAEPRSSIQYRNVFPGEDGWG
                     "
     gene            14834..15988
                     /locus_tag="PLM79_10760"
     CDS             14834..15988
                     /locus_tag="PLM79_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOV86834.1"
                     /translation="MRFEFAAAGRILFGPGTLEEIPSLVDDLGKKALLVHGRGRERAR
                     RLEEVLHPKGFEVVTFSVWGEPTTDLVEEGVRLARDEKCRVVLGIGGGSVLDTGKAIA
                     ALGPNDGPLLDYLEVIGKGKPLLVPSLPFVAIPTTAGTGSEVTRNAVLASREHGVKVS
                     LRNASMLPRLAVVDPLLTHSLPPTVTASTGMDALTQLIEPFLSNASNPLTDSLCREAI
                     PRVARALPRAFRNGSDSRAREDLALGSLFSGLALANARLGAVHGLAGTLGGMFPAPHG
                     AVCARLLPLVFEANAQALRSRNPLSPVLDRLAETARLLTGKASATPEDAVNRLQSLCS
                     DLCIPSLSAFGVTSGDIPVVAARARKASSMKGNPVPLTDEELGEILLRAL"
     gene            complement(16010..>17588)
                     /locus_tag="PLM79_10765"
     CDS             complement(16010..>17588)
                     /locus_tag="PLM79_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86835.1"
                     /translation="YGACGLPYYVEGVFSDLEMLSRTPVGIPRDADFFRKVKGFKTLT
                     GVEALRIDRRQKTVRVKHLTTGAEEDLPYDKLVLSTGSRPLRPPISGLELDRVWFMRH
                     PEDARSLVKRVEEEGLRRAVVIGAGYIGIEMAEALIQRGLEVSLVEMADQIMPQFLDY
                     EIAALSAKHLRQKGVRVILGERVLGLEGESKVTGVRTDRQSVPADLVVIGVGVQPNEE
                     LAREAGLQCAPRGGIVINSHCQTSDPDIYAGGDCVVNRSTDPACRDLLFFPLGSTANK
                     HGRVIADHIAGISSPFAGVSGTAICKAFDYNIGRTGLTEKQAREKDPDIVTAIWSGPT
                     HPHYIPHNRPLVIKMVASRRDRKLLGVQVVGPGEAAKRLDVAATALFFQAGVDQIANL
                     DLGYAPPYSSPLDPLAAAAHLLLNKMNGIARSISPLEAKERMDRGDDILLLDARTPQE
                     FQKMRLPHEKVVHIPLGALRERANELPRDKDIIAFCMLSMRGYEAQRILNAAGFDRVW
                     FFEGGLVAWPFEVWTAI"
BASE COUNT         3425 a         5441 c         5090 g         3632 t
ORIGIN      
        1 gtttcaatcc acgcccccgc gcggggggcg acattgatct tcgggatcgt tgctgacgaa
       61 ggctttgcag tggattttcc gcgaacatct gcgcgggata cttccccttc gtgaacggcc
      121 ccaaacccaa tactcctaac aacctaaatt acctaatgtt ttcacttccg cgaacctgtc
      181 ctcagcccaa tgcctccaag agtctcgccc cgcctgcaaa atgcccccaa gcccaccaat
      241 gggaacttgc ggttcgcgtt ttcttctcaa accaccaacg gttcttcggg atcgaccacc
      301 tttttcgctc ccacatgttc cacgcggcgc ctccagttgg ccccgaggaa atagaaacgc
      361 aggctgtcct tctcgggatc gatgagtttg agcaggcggt ctcgcaggcg aacccactga
      421 gccggatcca aggagcattc gaagaccgag aactggaccc tctgcccgta atccttgcat
      481 gctttggcga ccctccggag cctgccttgc ccctccgaat ccatggtcga aacgtcgtag
      541 ctgaccacaa ccatcatgac tcatcctatt tccagatgaa cggcggatac gcatccagct
      601 cacccctcaa ggaccgggcc aggagcagcg cctggatgtg aaagagcatg cccacgcgga
      661 ccttctctcc cagaaaagga tgcagaatct cctcttgttt cctgttttgg tacgcgacga
      721 tcacctcctt tctggtttca tcggacatga tgacggctcc cgtatcggtc tttttgaagc
      781 cgcctccctg gactttcttc aagttgatca gggacagagc cagcctgtcg gccagaaaag
      841 gccgaaactc ctccatgaca tccagggcca gtccaggccg accgggccgg tcccggtgaa
      901 gaaacccgac ggccggatcc aggcccacgc attccagggc cgagcgaaca tcatgcatga
      961 gcaaggtgta caggaacgag agaagggaat tcacattgtc caggggcggg cgccggtttc
     1021 tttcctggaa cgtaaaatct tccttttgag cgaggatgag ttcccggaag acgctgaagt
     1081 aaccatgggc cgcctccccc tcgattcccc gcagagcatc cagggtggtt tgaaaaccca
     1141 gtcgctccag gcactgggtc agcctggatg ccgcgtcctg gatggctgga gcgttcacct
     1201 tgtcttcatg gtcccgaagg accctgcgga gcaccgtacg gcaattggcc agttttcccg
     1261 tgagaacgga ggcggcaatt tccgcgctag actgcggatc gtcggccttg cggtactgga
     1321 tccgccgcag caggacattt cccgagaccg gccccaggac tcgggcaaga aacctgccgt
     1381 gctccgtcag gaaactgatg gtcacgtcgt tttcggcgca aaaccccagg agaaagggac
     1441 tgcacaggac attgccgaag cagacgatgc cccccagggt gtgaacggga agcctcagtc
     1501 ggacctcccc gtccaccttc accgccaccg tttccccttc cttgctgagg taggcgccct
     1561 gggtcgtcac gaacagcgtg ttgaggtgtt ttctcatcct tcctccaccg ccttcgaaag
     1621 gtaagcccga acggaccgtt tgccgtcgag cagtcgcggc aagcaccggt ccatgaggga
     1681 gcacgtcctg cactttttgg tataaaccgg cgggggtgtg ttgccggact cgatcaatgc
     1741 atggagttct cgggcgactc tttcggtttc ccctcgaagg tccgagtcga acgaaacatc
     1801 cagccggtgc cgggtccgcc cgtagaagac ggaccccgtg ggaacgtcga ttcccaacat
     1861 ttcttccagg cagagcccct gggcacagag ttgcaccttg tcgctctcgt cgggcttggg
     1921 ttttcccctc ttgtgttcca cgggaaaagg ccgccacaca tttttttcca ctcgatggaa
     1981 ttccaccacg tccgcttttc cgacaagccc caaccggaga gaccgaagag gcagccctcg
     2041 gacgatcctc acgctccccc gggattcctc ccccacctgg tgcacccgct cgtgcatgac
     2101 tcgcccctcg gcggtgaaaa ggttttcgtc ccacacctgc tccaggtgga tcagggcgca
     2161 ctgccgccga cagaacacca ggtgctgcag ggcggaaagg ggcaggaggt cgtcttcggc
     2221 gtatttcatc ggttgctccc gtaccggccg tccggaagga ccccatgggc ttcacggctc
     2281 accgaaagta tcacccgcac ggcctctcat ttcttaatcc gaagcacttg aacccgtgca
     2341 ccgagtctcc ggaggccatt ctcggcggtt tcggatgtgc tgcggcagag gattcctggc
     2401 ccttgttttt caaaacgcat gataaagcct ttccgtgaat cccccgcctt tcaggaactc
     2461 ccgctccccc gagcgaagct gccgatccaa cagggaatcg gtctggtgac ccgggcaaac
     2521 cggggtgttg gcaacagttt caggcagggc cggaggttcg atgaaaaact catggggacg
     2581 ggtacgactt atccctccca ccgccccccg ggaccggccc ggcttcatgg tagtcctttt
     2641 cccggagggg aaggccgcgc tcccgcagga agtcccgata ggcttgcagg agaaccttgg
     2701 gactcgcccg gaccggccta accagcttca gttgcatgtt cttataagtg cgcgtaacca
     2761 tgatgccttc tgtgatgttc cgtcagccga tttgcaccac ccggactacc tgctcgtgag
     2821 tccgggaacg gttgcagaat accactgtgg catcatggac aatttccacc atcgggcaga
     2881 acaccaagtc ccggtagccg ccgtggggcg ggatgatctt cttggcccca cctttctgcc
     2941 tgttttcggg ttcttgcata ggggcctcgt gagactcgtg catccatctc ccgcctgcat
     3001 tttccaatca tgccgggtgc ggcaatggaa tggggcgacc cctcctggcc tatttgggct
     3061 ttaatgtttc tccgcctgcg agcgcggacg catctgaaat caaggcttcg tgataagccg
     3121 gacacccagg ggcagatttt cgtcgtcaat ttccaggaca tagtctccgt aatcacgggc
     3181 cggtccggaa tcgtccttgc gcttgatgcg aattctgtca aaaagctcgt gtgacggcac
     3241 actcccaaga ggactctcgt gttcaaaaat gacgagcttg cgggcagcca tgcttcctgc
     3301 aggtcgagcc gccgattggt cgaactggaa agcattcatg agagattccc agagcagatc
     3361 caaatcatcg tcgccgaacc ccgtgccttt tggcggttct gccaggaaag gattgacgaa
     3421 gccgtgagcc cggtaaagcg catacggcac cgtatatttc ctccccatcg ttcgttcttt
     3481 ctccttatct gcttcgttgg tgacggagct tctcgtgatg gcatgctcga gggcgacgat
     3541 gggatggatg gatcgcgcaa aggacagttg aacgggacct cgcacctggc cgcagttgat
     3601 ctcggtggac atgactgctc cgaaagtacg gatatcgtag aagttgcggc acatgaaatc
     3661 cgtcacttct tttgctttcg ccacatcttt gggcagtttc ttcggttccg gcttttcccc
     3721 aacggcctcg taagcccgcc tgtgcgtcgc gtttagcacc gccccttccg taaaatagat
     3781 ttcgtgaggg ggactgttct gatgtttgag accgacataa ttgcggattt tcctcttcag
     3841 gcagacatcc gtaacgaggc cctccccggt ttcgggatcg atccggggca gatttccggc
     3901 gtcggggtct ccgttgggat tggcatcctt gacgtcgaat agatacacaa agtcataccg
     3961 cttcatgatt cctctccttc ctcatctttt tctcgatcct gcttcgaggt ccacagatcc
     4021 ctcatctggt ggtagtagcc aagggcgaaa agtccctgat ccgacaggtt caaatgggag
     4081 ggaaaatccg ttagcggttc gagaatctcc cgcaccagta tttcccgccc caccttgcct
     4141 ttttccattt ttgccaggtg atgctgatag gtccgaagca gcctgggaaa gacagccccc
     4201 ggcgtggccg aagcagcgct gtagaatcgg tcgcgaatgg tggcattccg cgttcccaag
     4261 gcgtcgccct gggttttctc cagcgcggca aaaagccgtc ccaagcggta ggccgcgtct
     4321 tttcgattca catccaaacc catactgacc tccttgccct ggtttctcgt caggtatcct
     4381 ttgatgatgc tcgcacggat gtaattgacc attctctctg tatggaccct gcgcatcacg
     4441 gccgtgaaaa ggcccaccgg gtattgtgag ccggtgatga cggcccgcaa caaaggtccg
     4501 gccaggatgg gcggaatctt gtcgcgatcc gcccttccgt ttctgcgagg acaagtttca
     4561 tccagtagtt gccaaaaggc cgggaattca ggatcggggt gtctcgtgtc ctccccgtac
     4621 tcgcggctga tttccatatc ggcgaaatga cgtcgcagat tgtccagaag atcgctgacg
     4681 gtgttccgat ggaaaaaccg caaaccgagc cggcccttgg cctgtcccgt caaccctaga
     4741 atgaagaaag gcgttctatc gggagcagcg tcgatttccg agtacgcttc cctcccctcg
     4801 cggagggctt tgaaaaagaa ctcaattttt tgccttaggc tttggtcctg gacctcctct
     4861 tgccctgttc ccatggagcc atgcagcgca aaccgagcaa aaatatcctc ggtgaccgag
     4921 ggctccttgg tccagaaaac cacggtggta tccgccaggg aaaaccggtg cttgtcgctt
     4981 tttgaaccga ccagaagagc gttgagagcg gttgtgtacc ggaaggcggc atcttgggaa
     5041 atcggagcat tgaagccctg ttccccgtcc cggccatagg aggcgaaggc atatttgtcc
     5101 ttgtcgaatg cgacgatcgg ggcacccgac gtctggcttc cccggactcc tttgatcttg
     5161 ggttcatgaa gtcttgcgat gggtttgaac tggcaggtga tcaggcactg accatataca
     5221 ccgtctttgg tctttgcttc ctgttttccc tcccaccacg cgcgtattcg tggccgctca
     5281 tgcaggtatt ccgttttccc caataactgg attaccccct gaccggcggc aaattcccgc
     5341 cagtcctcat gaaccaaagc ctcctccggt ctccaggtcc ggaggaaatt gcagaacgcc
     5401 ccatagtccg gatcgctgat ttcggactca acctcaaggt gatatttctt gaaggcttca
     5461 aattcccttg ttgccggtga aagaccggct tccttgtcgt ttcccgccat ggctcccacg
     5521 agaaaaggga gatcattgcg caagaactgc accttacggt gaacggagtg ttccgtcacc
     5581 ttcccggttg gcttgtcgcc cccgggcacg atcatcaggc gaggtttccc ggtattccca
     5641 tcgcgaaggt cttctattcc gtgcagcgtc ccgtcgttct tgatcacaag ccggaatgag
     5701 atcctctgga ggctgtaccc cggctgggaa atcccatatt ccgggtcgcg gacaagtcga
     5761 tcgtacaaat cgtacaggga ttgaagaatc atcgcatcag gcctcctccg acaccaaggg
     5821 cggcacctcg atggcgccgt cctgcatttt ggcggggaaa aatcggggct gagcctgcag
     5881 gcgacgcccc tggttggttt cgacaattct tccctttgga ttcggggtga acacaatgtc
     5941 gtgcagcatg taccccaaat cacgctcacc tcgcagagat tcatgacagg caggaaattc
     6001 ctcgaccagc gagaagtctg ccggaaactc acgagtcccc agataggggt gatggtaaca
     6061 agcccctttt cgcgctcttt ctttaaactg gcgaaggtgc ttggcctccg gagcctcgat
     6121 cggtttccct cccttcagtt ccggtttgac cacctccaca tgggcttcaa ttccgtaaca
     6181 cacatccttg agaatcatgg cagctctctg ttcgcggtga tcttccaccg caatccccag
     6241 gctgcagcac ccggctttca gcccggacac gacacttcgt accgagattt tgcagtcaat
     6301 ctcgtttcgg cggatgtgag tgaaacgaat cgggttcaag acccgcaatc tgtccaccac
     6361 ccagcgcatt tccggcttcc agtaaatggc ctcgagaatc ccccgggccg cactcggcgt
     6421 gataacctca tagctaaccc gttcgacctt catctcgggg cgattgaacg atgcaaactc
     6481 accccagacc agcaacttca ccccgtatgc cacatcacac ctccctttct agacgaatac
     6541 ccccgccggt tcccgggcat ccaggaccaa gccgatatcg tcggaataat gaagttcggg
     6601 gctcaccagc accgcaatcc catcgtggat cagttgaatg ttcgtcccca cgtgcttttg
     6661 ccataggccg ggagggacct gaacaaggaa gggctgcagc ctgcgaagaa cctcggtagt
     6721 gggagcaggc atggcgcgca ggcgctcgca caggcgtcgc ccttcctcac cccatgggat
     6781 gatcaccggc cggtaggccg cttcatcaat gagccggaat tttccggcgc tccgtccaaa
     6841 cccgaggtta aaaggaaaac tccggtcatt gtggagagaa aactcatcca tgatcttctt
     6901 ctcgtcccaa cggtccctct gatcccagta atacagtttg aagtagtgtt cgacggcctt
     6961 gagatccaac ggattgtcat gaccggagag gatctggccg gcacactgaa tcgtgtcggc
     7021 aatgaatctt tcggacttgg tgtgttccga tcggaagatg taggtcctcc ccactccggg
     7081 caatttgccg tttcgattgc agcggcctgc tgcttgggct atggagtcga tcccggccag
     7141 ggcacgataa accacgggga aatccacatc gaccccggcc tcgatcacct gggtgctgat
     7201 cacccggcac actcgattct cttccaggtt ttttcgaagt ctttcgagtt tttccgatct
     7261 gtgcgcggca cacatatttc cactgagatg gaaatggcct tcggcacttc cgatggcttg
     7321 gaacaatcta gccgcatgac tccttgtgtt cacgatacac aagactcttg gctctgtcat
     7381 gaggcgattg gacagctctt catccttttg gcccccaagg ttctcgaccc ggactcgttc
     7441 caactccctg taaagtcgaa cggggtccgg aatgatttca tgcggtttcc tgagaccgat
     7501 ctcaaagtcc ttggttcttt ggatctcggg ttgagtggca gtgcagagca gaacggttgc
     7561 ccggtaattg tcggtcaact cctgtaaagc acgcagacac ggcttcaggt actcgacggg
     7621 cagtgtttgg gcttcgtcca ggatgatcac cgaccgagcc agccggtgca gttttcgaca
     7681 gcgggatgtc ctgttggcaa acagggattc atagaactgc acggaagtgg tgaccaccag
     7741 gggtgcatcc caattctcgg ccgccagccg gctcatcgtg gtctcgttgt cgggatccag
     7801 gttgctatga tgttcaacca ccgggtctcg acctagtccg tcagccaagg gtttcatcgc
     7861 acgacgaaaa acctgcgcgt tttgctcgat gatgctcatc aaggggacga catacacgac
     7921 ccgttgtagt ccgttgatga tggcgtgacg taacgcaaaa gcgagtgaag ccaacgtttt
     7981 gcccccaccc gtggggaccg acagcgaaaa caatcccggc tgcctctccg ctgcatgcag
     8041 gcacgcattc cggaccaggg tccgttgcct gtcgacggga ttaccccggg gatcgagtct
     8101 cccaatataa tcggtcagag cgtcctccat gtgttttagg acggtgttcg gccatccctt
     8161 ccgccaggct gcctggtcgg gattcatgaa cctttcggtg tccagaaaat cggcgtccac
     8221 aagacacgaa aaaaccattc gcacgaagaa cgctacggag aatgcatcct tcctggacag
     8281 cgcgattcgc aagaaatcag gcccctcgag cttcggaagt tccggcaaga ccttgagacc
     8341 gtgttcccat ggtgatagac tcttttccag acgcttctcc tggcaactcc ctgtggaacg
     8401 accatccagc aaccccgaat gatgacctgc aatcacgtag gctaacagat ggcccaacag
     8461 gttcggcatg cgagcggcgt attgggcgcc cgcactggaa tgatccaccc gcccgggtgc
     8521 gttttcgatg ctcgcgtcca cattattctg ggtttttagg tactcttgaa acgcgagaga
     8581 atatttccca acatcatgca gccacccggc atggatgccc cattcgcaag ctccaaagga
     8641 ggcggcaaat tgccccgctc tttcggcgac attcctgagg tgttcctcga ggggctgcca
     8701 ctctgagggc ggtctttcgg caaggctgtg agcataatgg ctctgacatg gcatggctat
     8761 cgtgcctttt atggggggcg ccaacacacc acaatccggc tctgagcctg gaaacggcaa
     8821 agatcgagcc tcggtgatcc gtgaggacac gggctcccgt gctcttacga gcaattccaa
     8881 agcaatggga cacaaccggc atcggggaag gcccctgggt gcggtcacgt cgtttcaggc
     8941 gggagtcccc ctccggatga ccagaccgga ttctgccgca tatttgaggt taccagggat
     9001 tttttggtga cggcagggta ccacaaggaa cggaggttgt ccacctgagg ggacaccaga
     9061 tgcaggggga gcccatccct gaaaaggccc gggaccggaa tctttgaggt accggctccc
     9121 ggctaacggt tcacggctcc ggggcgcttt cccctacttc atacgccggc cccacggcag
     9181 ccggaggggg gtgaaggacg gcacagccgg gcccgccttc ccggaggaag acctaatccc
     9241 accaaaggct gtccaaatcc agctccagtt ccacgaaggg ttccgcccgg acccgggcgt
     9301 tttcggcaac ggtctcgatc agcagccatt ttcccgattc cagccggaaa acctccaggg
     9361 tcttgacggc cggatcgatg atccagagat gcttcacggc gtgacgggcg tagaccggca
     9421 ttttccgggc ccggtccttg ccgaccgtgc ccggggagag aatctcgcag acccagtccg
     9481 gcgcaaccga aaaccagttc tcggcgggca tgccgggaaa ccgctccttt cgccacccgg
     9541 ccaggtccgg caccgggagg tcctcaccca gcaggacttc gggttcatga aggatgaccc
     9601 aacccccggg cccgcctcct tctccaaact gaaacggagg cagcagcctg cctcccagga
     9661 ccgaggccgc cagggcatgc cgggccgagg gtctcggcgt gatgatgagt tccccgccga
     9721 ggatctctcc ggtcatgttc tcgggaatgg aaaacaggtc ctggtaggca gctttctttt
     9781 ttgcgggatc agccatggaa cgctcctcgc cctcgggttt ccgtccagga tacccccatg
     9841 ccccgggggg atgcaaaccg gaatccacgg ggcggccgcc gcctggcgca cggtctcccc
     9901 aacggagtcc ggcaacggag gggatgggga gcagggaaac cgtgggtgac cggtcgacag
     9961 gatcgaagag cggagcagga tcggggaagc tcgatgcacc ctatgcagcg tcccgtcgac
    10021 tcccggaaac ccgaggctcc gtgacgtccc ggtcgggtcc cgacctcgtc tctcagtccc
    10081 ggggcacgcc gtcctcgctc cagccgcgga tgcggtagta atcccggagc atggtttcca
    10141 tctccccggc ggtgagggcc tggccggtgg ggagagcttc ccggtgaagc cggcggggca
    10201 ggcggtcctg ctcgggatcg aggccttccc gaaggttgaa acggcgggtc atgtcggcga
    10261 tgttggatgc gatctttccc aggtcttccc tcgaggacgg aagtcccgtc accatgccga
    10321 cgaccttttc cagttcctcc caggggtaga gatcccggta aaaacggcaa aggatcaggg
    10381 tgtcgaaaat ggtgaggcga tcctcgtagt cgatgagcat ccgggccttt ccctcgacgg
    10441 cgtcccgggg aatgagtccc gccagctcgg gcttgtagaa cgtggttcgc aggtggcagg
    10501 cgccccggtc ggaagtcccg taggccagtc ccatcccttt gagggtgcgg ggatcgtatc
    10561 ccgcgggttc catgcctttg acgtgcaccg ccaggtcctc gaggtcccac tgccgggccg
    10621 cgtgggcgat cccttcggcc aagacgtccc cgatcccttc ccggttggcg atttgcacca
    10681 ggagccgcgc gatttggtcc ggttggccgt aatcgatctc gtagcggacc tttccccggc
    10741 gcacggcttc gatggtgaag gcgcaaaggt ttccggccgt gatggtgtcc atgcccatgc
    10801 ggtcgcagag atcgttgagg taggcgatct cttcgatctc ttcgatcatg cacaggcccc
    10861 cgaaagcata gatggtttcg tattcggggc cctcgatgac cagccccttg cggcgcccct
    10921 tttgaatggt cgccagccgc ccgcacgcca tgaggcaccc ggcgcacgca tgggggcgaa
    10981 cctggtgctc ccggtggaag gtctgcccgc tgatcttttc ccagtgctcg cagctcccct
    11041 ccgtccagta tttggacggg aacgcttggg cttcgtttag gatcttcacc atcattgtgg
    11101 tgcccaacgc ccggtaggct ttcactccgg cgttgtcccg ggcggttttc gagaaggcct
    11161 tgccgtattg agccagggcc ccgggttggg ccggctccct gcggcggtct ccctggaaaa
    11221 ccaccgcctt caactgcttg gagcccatga cggccccgac tcccccacga cctgcggacc
    11281 tccacttgtc gttttcgatg agggcgaatc gcaggagttt ttctcccgcg gggccgatga
    11341 ccagggcccc cggtttccgg tacccggcct tgttgggagc aaaccgggcc cgggcttccg
    11401 cctcggtacg aaaggtgtcg gccccccaga ggtctcccgc ctcgtgaaac cgcgctcccc
    11461 ggggatccac cgacacgatg gtggggcggg cggccctgcc cgtgaagaca acggcgtcaa
    11521 acccggtcga atccacggct tcggggacct tccctcccga gtaggattcc acgtaaaggc
    11581 gggtcaaagg cgacttggta tagactccgt atcggctggc cccccatacc gggctttggc
    11641 agcagggccc ggtggcgatg atgaggtggt tttccgcgga aaaagggtcc gcgccgggag
    11701 gatttctttc catcagcagc cgggtggcaa gccccttgcc tcccaggcat tcccggagca
    11761 cctcctcggg aaccggttcc accgtagagg tctcccggct caaatccacg gcaagaatac
    11821 gaccgtaaaa gccgtgcatg gctgactcct tttgaccggg agcgtcatgc atcccggggg
    11881 atgaacgcgc catagagaat gtgtgacggg agggaaagag caggaaagag caggagcgca
    11941 tccgggcgga cacgccccct agatctcgtc tcgctcctcg agcttggtga gaaaggcctc
    12001 catttcgctc acacgggcca ggaattggtc ccgtcggtcg aaatcccact gggagcagaa
    12061 ggcgggcctg ccgtacaggg ccctctgggt gcacacggaa cagctgggct cgcagcgacg
    12121 gatgggatcc tgaatgatgc ccgccgcctt ttggggccag aggggatccg cgaaaagcac
    12181 tctcgccagc cctatgaggt ccgccttgcc tttttggagg atctcctcgc cggtttgagg
    12241 ggactgaatt cttccggccg tgatgatggg ggtgttggga accgccttct tgatggcctc
    12301 ggcaaaatga gtcatgtagg cttccttctt ttcctgttcg aggtactgaa ggagaaagaa
    12361 gcagtccagg gtgcccgcca tcaccgaaag gtatgcgatc tccttctttt cgagctcccg
    12421 ggcatagatg ctggtttcgt ccgttcggag ggcctggccg gggagttcgt cggagaggaa
    12481 ccggtacttt ctcttgagca tcttgaacag ggagaaggac agcctcagtt ccgggagcca
    12541 ttcatcggcc atgaagcggc tgcctacaca atagtccttt cccacggcgt caagaaccgc
    12601 atccaccact ctcaggggaa accgcatgcg gttttccagg gaccccccgt agtgatccga
    12661 gcgacgattg gtgcggggag aaaggaattg ggccaaaaga tagcccgccc ccccgtgaat
    12721 ttccactccg tcaaacccgg cttccttgac cctgcgggcc gccgaggcaa aagcgtccac
    12781 cgtccgatcg atgtcggcct cgctcatttc ccgggggacc acatccccga tttgcaggga
    12841 ggaaggcgcc agcctttcgg cggtgaacgc aaatcgaccg gcatgattga tttgcagaag
    12901 ggcgtaagcc ccttgggcac ggatggtttc ggccagtttg gcgagaccgg gaatgtaccg
    12961 gtcgtggtcg acccgaagca tgtaaggagc gcccagcccc aggaaatcca cggcggcgtt
    13021 ttccaccacc accatggaaa cgccggatgc cgccaggagg cgatagtgtt ccaggagaag
    13081 ctcgctgacc gtcccgtcgg gattgccgta ccccacgtac atgggggcac gggtgatgcg
    13141 gttcttaaga tacaatccct tgatgcggat gggcgtgaag aggagtggcg tctgctccat
    13201 gactccttcc tttcctcatg cgacaaagca tcccgggaaa ccttctccct ggcgcgcgga
    13261 aacaacccga acgagaggcc gatgcagtgt gggacgaaag cccgacgccc tccattatac
    13321 gagctcccat ggagcgaaat caaaccgatt ctcacccccg ggagcgggat tcaagagccg
    13381 ggtcaggccc tccgggttcc cctcccccgg tggggacagg gggagggggg aagagaagtc
    13441 cgggtccaaa cggctgggat gccctttctc agcggccctg gtcgggatgg aagtagggag
    13501 gatacggact ggttcggtcg ccggttcgag catcatgagc cccgtaagcc ccgggcgtgg
    13561 tgtaggaagg accgaccacc gctcccattt cattggtatt tctcggctcg agggtaagcg
    13621 cctgcccttg acgggattcg cttcgttcca tgggagccgc agctgccaag aacaagagtc
    13681 ccaggatcat acagaatcgg tagaacgatg ccatcttcgg tctccttctt ctactcttgg
    13741 tgccgaatcg ttgcgctttc acagcataac tttttgccct gttttgtcaa tccacaggcc
    13801 cccggggggc gggggcaggg aatttttctt tccatgatcg cgttttggaa gtagaatgag
    13861 tccgtacgat tctgcactac ggttttcttc ctttcagcct tcatcggggc gcacagggtg
    13921 aaggaatctg aaagaatcgg ccggccgcat cagagcattt cgagtgggac ctttatcctc
    13981 ctgcatcccg aaggggggct gttgcaccgt gaggatcgac gaggaaaaag ggggcatctg
    14041 tccgctgaac ccgagggaag cggcggaggt ttttgagctg ggaaaacggc cctggattcc
    14101 gggggtcggc agggacagga ccatggacaa gcaggagaag actctcaagg ggttcaccgt
    14161 ggcaaaaagg tgtgacattt gcggatacag gaccaagatt acttccgaca cgggcgaccg
    14221 ctgcactttg aagtgcgttc attgcggcaa ggaatacagc ttctatcaac gacgtccctg
    14281 acgtttcgcc gtcccgacac gagtccccgg tcccaacggg tttctcctta catgagagtc
    14341 tccccgaaat cttcgtatcc gaacccggca gttcatccca ccgtccgccc cgcatcacga
    14401 aaaaggtcgc cgggcaagcc gtaagcggca ggcgggcatc tttcatggga atcccccggc
    14461 aacttcctcc tcggggaagc cgggtgtgtt ttccgccgac attcgacagg aaggaggggt
    14521 catgttcgtt gtccatgtct ttgtgcacgt gaaaccggat cgggtggaag atttcaaagc
    14581 cgcctcgctg gagaacgcag cccggagcgt gttggaaccg gggatcgccc gtttcgacgt
    14641 gattcaacag atcgaggatc caactcggtt cgtgctggtg gaggtatacc ggaccccgga
    14701 cgacccggca aaacacaaag agacggctca ctaccgccaa tggcgggagc gggtcgagga
    14761 catgatggcg gagcctcgat cgagcatcca gtatcgcaat gttttccccg gcgaggacgg
    14821 gtggggatag gccatgagat tcgagtttgc cgccgcaggc cggatccttt tcgggccggg
    14881 gaccctcgag gagatcccgt ctctcgttga cgatctggga aagaaagccc tgctggtcca
    14941 cggcaggggc cgggaacgag cgaggcgtct cgaggaggtc cttcacccaa aggggttcga
    15001 agtagtgacc ttttccgtat ggggggaacc gaccacggac ctggtggagg aaggagttcg
    15061 cctggctcga gacgaaaagt gccgggtggt cctgggaatc ggcggaggca gcgttctgga
    15121 cacgggcaaa gccatcgcgg ctcttggccc caacgacgga cccctgctcg actacctcga
    15181 ggtcatcgga aaaggaaaac cgctgctcgt tccctctctc cccttcgtcg ccattcccac
    15241 cacggcgggc accggttccg aagtcacacg caacgccgtc ctcgcctccc gggagcacgg
    15301 ggtcaaggtg agcctgcgca acgcttccat gctgccccgc ctggccgtgg tggatcctct
    15361 tctcacccac agcctgcccc cgaccgtcac ggcgagcacg gggatggatg cccttaccca
    15421 gctcatcgaa cccttcctga gcaacgcgtc caacccgctc accgattccc tctgccggga
    15481 agccatcccc cgggtggccc gggctctgcc ccgcgccttc cggaacggat cggactctcg
    15541 cgcccgggaa gacctggccc tgggaagcct gtttagcggg ttggccctgg ccaatgcgcg
    15601 gctgggggcc gtccacgggc tggcaggaac cctgggcgga atgtttcccg ctccccacgg
    15661 cgcagtatgt gcccggctgc ttcccctcgt tttcgaagcc aacgcgcagg cgcttcggtc
    15721 ccgaaatccc ctctcccccg tcctggaccg gttggcggaa acggctcgcc tgctcacggg
    15781 caaggcatcc gccacccccg aagacgcagt gaaccgcctg cagtccctct gctcggacct
    15841 gtgcattccc tccctgtccg ccttcggcgt cacctccgga gacattcccg tcgtggccgc
    15901 ccgggcccgg aaggcaagca gcatgaaagg aaatcccgtt cctctcaccg acgaggaact
    15961 gggggaaatc cttcttcgag ccctctaggg gaaggaccac ggaccgaaat cagatcgccg
    16021 tccagacctc gaagggccag gccaccagcc ccccttcgaa gaaccacacc cggtcgaagc
    16081 ccgctgcgtt caaaatcctt tgcgcttcat agccccgcat gctcagcata cagaacgcga
    16141 tgatgtcctt gtcccgggga agctcgttgg cccgctccct gagggccccc aagggaatgt
    16201 gcaccacctt ttcgtgggga agtctcatct tctggaattc ctggggcgtc ctggcgtcga
    16261 gcagaaggat gtcgtcccct cgatccatcc gttccttggc ttccagggga gagatggaac
    16321 gggcaattcc gttcatcttg ttcaacagga gatgagcggc ggcggccagg ggatccagcg
    16381 gggaagaata aggcggcgcg tacccgaggt ccaggttggc gatctgatcg acgccggcct
    16441 ggaagaagag ggccgtggca gccacatcca ggcgcttggc cgcctcaccc ggcccaacca
    16501 cctggacccc cagcagtttt cgatccctgc gggatgccac cattttgatc accagggggc
    16561 ggttgtgggg aatgtaatgg ggatgagtgg gaccggacca gatggccgtc acgatgtccg
    16621 ggtccttttc ccgggcttgt ttctccgtca ggcccgtgcg gccgatgttg tagtcgaagg
    16681 ctttgcagat ggccgtcccc gaaaccccgg caaacgggga agaaatcccg gcgatgtggt
    16741 cggcgatgac cctcccgtgc ttattggccg tggaccccaa gggaaagaac aggagatccc
    16801 ggcaggcggg gtccgtggag cgattcacca cgcagtcccc tcctgcatag atgtcgggat
    16861 cgctggtctg acagtggctg ttgatcacga tgccgccccg gggagcgcat tgcagcccag
    16921 cctcccgggc cagctcctcg ttgggttgga cgcccactcc gatgaccacc aggtccgcgg
    16981 gaacgctctg ccggtccgtc cggacccctg tgaccttcga ctctccttcc aggcccaaaa
    17041 ccctttcccc caggattacc cggactccct tttgtcgcag atgcttggcc gaaagtgcgg
    17101 caatctcgta gtccagaaac tggggcatga tctgatcggc catctccacc aggctcactt
    17161 ccagcccccg ctgaatgagc gcttcggcca tttcgatgcc gatgtacccc gctccgatga
    17221 ccaccgcccg ccgcagccct tcctcttcca cccgcttcac caggctccgg gcatcctccg
    17281 gatggcgcat gaaccagact cgatcgagct ccaggccgga aatgggcggg cgcaacgggc
    17341 gactgcccgt ggaaagcacc aacttgtcgt agggaaggtc ttcctcggcc cccgtagtca
    17401 agtgtttcac ccgaacggtc ttttggcgac ggtcgatacg cagggcctcc acccccgtga
    17461 gggtcttgaa tcccttgact ttccgaaaga aatcggcgtc tctgggaatt cccacgggag
    17521 tccggctcag catctcgagg tcggaaaaga ctccttccac gtagtaaggc aacccgcagg
    17581 ctccgtag
//
LOCUS       DAOOVC010000077        17478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2229_length_17478_cov_5.919991, whole genome shotgun sequence.
ACCESSION   DAOOVC010000077 DAOOVC010000000
VERSION     DAOOVC010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2229_length_17478_cov_5.919991"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLM79_10770"
     CDS             <1..242
                     /locus_tag="PLM79_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOV86836.1"
                     /translation="PAWIVAVSAQEATCRRLQFSSGVYPVCEPEHPENWNVYIAEWLK
                     ARGMGGKFAVMTEGPSRRHPETNHRLEIVDLGRGN"
     gene            491..1600
                     /gene="ydiK"
                     /locus_tag="PLM79_10775"
     CDS             491..1600
                     /gene="ydiK"
                     /locus_tag="PLM79_10775"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HOV86837.1"
                     /translation="MTESHRDIPRIFLAVLFIGAIIGTSLWILRPFLGAAIWAVTIVT
                     ATWPLMVTIQNRLWGRRSLAVVLMTVILLCFLVVPFWLAIGTIVSNSDRIAGWVRSMP
                     EFEVPPPPAWLDRVPVVGPDLVTAWEKVAVAGIKDFMKKLAPYGGSAIRWFITEVGGF
                     GVLVLQFLLTVAFSAILYARGEHAASKVRLFGRRLAGPRGENSILLAGQAVRGVALGV
                     VVTALAQSVLAGVGLAIARVPFAAILTAVMFMLAIAQVGPMLVLIPAIIWLYWTSSAG
                     WGTFLLIWTVVVGTMDNFLRPVLIKKGAHLPLLLIFAGVVGGLMAFGLIGIFVGPVVL
                     AIGHTLLLAWIDEEAAPTPPSPPPAQATEGGKPDS"
     gene            complement(1771..3453)
                     /locus_tag="PLM79_10780"
     CDS             complement(1771..3453)
                     /locus_tag="PLM79_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86838.1"
                     /translation="MEETTRSNAARLAIHSFRIAADALLLMGRFHMNARSGQVLQDAL
                     RTLSPEIYGTMNDPRSIELKGLEYVMERLPKGIEECSRFVMTSEEDLGDTPFVMLQPL
                     KRRRTSYRISESEMCFVITRGFSEIYDILTHLTFLYIEAKNIHSKMRDQEGNVTTEWQ
                     EFQKFMEERDPCDGVKLNRALWNLSIILGRTFHETRDSYEYFGRLKNEHKAANDLFEI
                     VHSLGTRMESATEKQADMEVRFTPDLSHVILHQVYGKKWAAGIRETLEALNLEHRPLH
                     IISSNLHSVANLLYGYGAVGGIAANECPPDLYSLVHVLRNRTNEVLEYAKNYGFYEVP
                     DRSGAHVDCQIIDTAKLNWEGFHPDLAWDGNAVGEPKPVILVMDYAFGTQAFELIDSL
                     LQPVMVDGVEKNLDVRSMSVMGKAGTLLGKKGDVMIATAHVLEGSAHNYMIENDLKEE
                     DFEGYRNVFVGPVITVLGTSLQNRDVLETFKSTSWKAVGLEMEGGHYHRAINAAMIRG
                     HISRDVKVRYAYYASDNPLMSGQTLASGSLGAEGVKPTYLITKCILDKILKG"
     gene            complement(3631..4917)
                     /locus_tag="PLM79_10785"
     CDS             complement(3631..4917)
                     /locus_tag="PLM79_10785"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOV86839.1"
                     /translation="MKIRERPLGRGLCLLTLLGVLGLAGLGAIPDAAGLGAGVSLAQE
                     APSRAGSVQPAGGPGLLGEEVTQPTGIAVDSAGNTYVTGQWVNGSGSSNFATIRYDSA
                     GKQKWVRYYNGPAKGNDGSNGVAVDSSGNVYVTGSSENDYGSDSFATVRYDSAGKQQW
                     AKRYSGPTRGSDRPSGIAVDSSGNTYLTGISQSGEQTYDFATVKYDSSGKQLWAKRHA
                     RSGSQLNYPVGLAVSKNSVYVGGSTRGGSGETDLTVLKYDSKTGKRAWAKNFNSGFGP
                     DNPAGIAADQKGNAYLTGWTGNGDFLTVKYDSSGKLVWTRTYNGPGQGYDYTKAVAVD
                     SSGYAYVTGQSLGAGNTTDFATIKYGPGGKRYWVQRFDGKGKGADTPAGIAVDSSGNV
                     YVTGQTQLGSGEDYGFATVKYDSSGKQTWVTYFSGP"
     gene            complement(5038..6474)
                     /locus_tag="PLM79_10790"
     CDS             complement(5038..6474)
                     /locus_tag="PLM79_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOV86840.1"
                     /translation="MAIQKASMQRGLRLAALCCAVGLAVPGMSFNTAAREAGLVAPEE
                     QATVRASEPVISPSKLLRTGGPSGGGPKVVADRQRVYVSGPLPNSSGNTDFTTIQYSS
                     KGKQGWVRRYNGPAGGEDVPQGMAVDGSGNSYVTGSSAGGSGPTDFATVKYAPDGRQE
                     WVRRYNGPAKDYDFPVGVGVDNSGSVYVAGDAGVSENRWDYTTIRYSPAGKEVWNKRY
                     NGPYQGSSSPAALYVDPSGYLYVTGHSQSGENTYDFATVCYTKTGHLDWVSRYARPDS
                     NYNFPVGLVESGGSVYVAGSTRTKSGNTSYTVVKYNRKSGKRAWARHYNSGQGDDDPS
                     AIAAGKNGSVYVTGTSGRGKFQTIKFNRSGDIGWTRSYTETIDYDRPTAMCSDDAGNV
                     YVTGESQGDGSGSDYLTIKYTPGGKVLWVRRFNGAGNGDDTPTDIAVDQDGYTYVTGW
                     SESSQGKNTFATIRYNPNGGQSWVRYFP"
     gene            6862..8685
                     /locus_tag="PLM79_10795"
     CDS             6862..8685
                     /locus_tag="PLM79_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86841.1"
                     /translation="MGKQAEHPGHPAGTGALWKRTVHSLLIVLVAVLAYSNTFDVPFH
                     FDDTPNIVANSLIRDFSNFLDPARARGHPYYDDFKMRYVGFLSFAVNYKLHGLKVPGF
                     HGVNLLIHVLNGLLVYFLVVATLRTPKMRPAVSGDEPVLSRETGTFPIPLFAALLFAA
                     HPIQTQAVTYIVQRFASLAALFYLAALLAYARSRLLSGQAPRALRRGLYFLGLLCTIL
                     AMKTKEPAFTLPLSITLYELLFFPAPSASRWKRLRLLLPFVLTLGTIPLSALDLSGAH
                     PSWLDQLEHKSRILTTLSRQDYFLTQLTVIPVYLRLLFLPAGQNLDHDHPVVRVFLDP
                     RVVLSSLLILCVLLTGVYALRASRFGDPRWRLTAFGIFWFFLTLLPESSIIPIVDVLY
                     EHRLYLPSVGAFLSIASGLSLLEGRPGRNHPRTRLGMVLVGAAVVFALSAATYARNEV
                     WRDEVTLWEDVVSKSPNKDRPHNTLGSLYLNRDRPDLAYRELCRTLEINPDYWAAHVN
                     LGSWYMMEATAAARRREPATRVLERVEKGCRFLRTGPGPGSGKRDDPFPPGVRPGAAA
                     LPGSQGGALKPLALNLPRSPARVVPNLLFSAAVRKPGSILH"
     gene            8880..9086
                     /locus_tag="PLM79_10800"
     CDS             8880..9086
                     /locus_tag="PLM79_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HOV86842.1"
                     /translation="MSVYKIIELVGTSDQSWEEAARTAVETASKSLRDLRVAEVAEMD
                     LKFEEGKILFRVKLKVSFKYEGRS"
     gene            9730..11985
                     /locus_tag="PLM79_10805"
     CDS             9730..11985
                     /locus_tag="PLM79_10805"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOV86843.1"
                     /translation="MIRREDALQYHIQERPGKIEVKSSKPCITQRDLSMAYTPGVAEP
                     CREINKDADRVFDYTVRGNLVAVVTNGTAVLGLGNIGPLAAKPVMEGKAVLFKRFADI
                     DVFDIELGSTDPEEIIRAVKLLEPTFGGINLEDIKAPECFYIEERLKEIMTIPVFHDD
                     QHGTAIISGAALLNALELQRKPIDQVKIVFSGAGAAGIACAELYIKLGVKRENVFMTD
                     SHGLVHHGREQGMNPYKERLANGSRPMTLAEVLKDADVFAGVSAPGVLTPEMIRSMAP
                     RPIVFALANPDPEIGYHEAVRVRDDLIMATGRSDYPNQVNNVLGFPFIFRGALDVRAT
                     AINDEMKIAAVNALADLAKEDIPDSVLKAYGGQVLRFGPEYILPKPFDPRVLVWEAVA
                     VAKAAVASGVARKPIYDWNEYRDALESRLSRSQEIMRRIIHRAQREPKRIVFLEGNEP
                     KILRACQVILDEGIARPILLGERSVIERNIVELGLELDKAVIVDPRNFERFDEYVTEF
                     HRLRSRKGITRKEATKLLTVNRNYFGAMMVRMRDADGLVGGINKHYPETIRPALQVLP
                     LEEKTSVIAGMYMMVFPKDVMFFADTTVNIEPTTEQLAEIAICAADTVIRLLEIKPRI
                     AMLSFSNFGSTRHPLTQKVAEAVKVVKKRRPDLKIDGEMQADTAVIPEILNRVYDFNE
                     LKGKANVLIFPCLDAGNIAYKLMERLGGARAIGPILMGTSRAIHVIQRNCDVEDIVNA
                     TSLAVIDAQEH"
     gene            complement(12110..12541)
                     /locus_tag="PLM79_10810"
     CDS             complement(12110..12541)
                     /locus_tag="PLM79_10810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86844.1"
                     /translation="MSTPWFELTLEFQRKLDYLRLATAVSRLVSEAAAREDLPGSLVD
                     DVELAVSEACTNAIKHSARAGGTARVVLRFRVYEGELVIEVSDRGEGFDIEKVPLPEF
                     DRHPEGGYGLYIIRTVMDEVRYTAAAGVNTLTMKKRLKRPR"
     gene            complement(12538..12897)
                     /locus_tag="PLM79_10815"
     CDS             complement(12538..12897)
                     /locus_tag="PLM79_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV86845.1"
                     /translation="MEILDEIVGKATVLKLNGRLDSASAGSLKDKVKDCAKKGRIHLV
                     IDMAGVDFVDSSGLGSLVACLRSVNKLGGDIRIAAPQSRVRAVFELIRLHHIFEVFDS
                     AGTAVSSFRDAGTGTVS"
     gene            complement(12881..14002)
                     /locus_tag="PLM79_10820"
     CDS             complement(12881..14002)
                     /locus_tag="PLM79_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86846.1"
                     /translation="MDPVIIENIFENLAVGLLVIDPRGEIILANSAASAILGRPGQVL
                     AGKGWADLFLCEENDSFTQVIVDVIWEERLNLRRTVPYVRADGGVLQLSIATSYIRRD
                     EKLAGIVVLLHDVTELHALHLQEKKILEDRNRLQMERTESLNKLAMAVAHQLRNPTTA
                     IGGFAALMLKKAEPGSLQAEYLQQILNSTRRLENLVRSVHDYASLPPVSLRRVPVDVL
                     RESMRRRVMEKSAECSREVELTIHADSLEVEMDPDLLAEALGEVLDNAVESVNPGGGR
                     IEIDLVRQGEMLVVAIRDNGGGIAERDLPYVFDPFFTTKAVGVGMGLCRARRIIAEHG
                     GSLEVESTLGTGTRVVVRIPGRTAAFLTTKGECRYGDLG"
     gene            14977..>17478
                     /locus_tag="PLM79_10825"
     CDS             14977..>17478
                     /locus_tag="PLM79_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86847.1"
                     /translation="MSPYEPHNGLTSSSEARRTRRAVEIARAVFPQAAARARSTKPEN
                     LSNAIRNLDEWLSRDRFADYREALLDLLEIAAQDPAVADELYDSFYRVMPFGTGGRRS
                     KVGIGPNRMNAYMAAMTAQGNAEFILGQLARQGTSVSETDLFAGAWDVREFHRYFAET
                     PALERIRKVIEDKCPALSGLSSRDLSEIAGLVYAGNHIRYVHPVAMRPTPWLSFLINA
                     WGRIVARGPFSGEAAVLNRCRRVLGGIVLSSSHNPYDNNGTKFYEMSGAQTPPHIVEV
                     LQRIGDAVQEIRYFGGDTHSRQGRKAAFEAARKSGMIVVLDEENLEQIDRFYVQNSIE
                     EIQSLYPPQEWKALATPSNPNDPYSPAFGRLIVSFNALNGTGSVGVLKILESAGFPVL
                     RSPGDLPTWEFTEGYGNVPNPEAEKTFNTGMQIGVRRSLAALLSGDLRGLASVSSLID
                     EDGNRVPYAAFSAAGSADEAIRSFREATGKAVRGLELAPSDSSESTLAYLKDHVLRNN
                     ICLLTDPDADRIGLGMQVFRVSEDRRSVRFQWISANDNDESGIVLFRYRLERMVEMAR
                     RGELIRFIEDRRREDGCPAEPGQNHQLVVVNTVVSNPLEAVIAERISSQLEELTGGRV
                     SVKAITHHVGFKFTGEIIDNIKRGNAGLPFEGITGELMTRAGIRGREAFFVLSSEEGE
                     GSLIGYRGSIDKDSGVTGLALAVLAAEQQNRGMTLYEYLMETYDRYGYCKAALEPMVL
                     TGEYGMTMIHDHIMGRMRDELLPAVRGGARPEWPLPDGSDRLVLVGGTDHLDLMKKSP
                     DEDPASWTPTRRNLPDDPARWPAAIRESINILEFYA"
BASE COUNT         3236 a         5641 c         5149 g         3452 t
ORIGIN      
        1 ttcccgcctg gatcgtcgcg gtcagtgccc aggaagccac ctgtcggcgg ttgcagttct
       61 cttccggcgt ctacccggtt tgcgaacccg agcaccccga gaattggaac gtctatatcg
      121 ccgaatggct caaggcccgt ggaatggggg ggaagttcgc ggtgatgacc gaaggccctt
      181 cgagaagaca cccggaaacc aaccaccgcc tggaaattgt ggacttgggc cgggggaact
      241 gaagggaggc cgactcgatc ccaacgccta ccccccttgc ccccgaggga ccggggggtc
      301 cgcaacgggc acgcaccgcc ggacccgatg atcacaccgg accggatgat cgggactgtg
      361 ggggccgaga ggctcgattc tcccggcctc cgggacccgg gcaggacctc gaggggcgca
      421 tggatcggcg atctcgaagg caggaaacgg gttcgagaac ctgccccggt agaaaggaga
      481 ggacccctcc atgaccgaaa gccaccggga cattccccgc atctttcttg cggtgctctt
      541 catcggagcc atcatcggca cctccctgtg gattctcaga ccgttcctgg gggccgccat
      601 ctgggcggtg accatcgtta cggccacctg gcccctcatg gtcaccatcc aaaacaggct
      661 ttggggacgc cgctccctgg cggtggtgct gatgaccgtc atccttcttt gctttctggt
      721 ggtgcctttc tggctggcca tcggcaccat cgtctccaac tccgatcgga ttgccgggtg
      781 ggtgaggtcc atgcccgagt tcgaggtccc gcctcccccg gcctggctgg accgggtgcc
      841 ggtggtgggc cccgacctgg tgacggcctg ggagaaggtt gccgtggccg gaatcaagga
      901 tttcatgaaa aagctggccc cctacggcgg cagcgccatc cgctggttca tcaccgaggt
      961 gggaggcttc ggagtcctgg tcctgcagtt cctgctcacc gtggcctttt cggccatcct
     1021 ttacgcccgg ggcgagcacg cagcctcgaa ggtgagactc tttgggcgcc gcctggcggg
     1081 gccacggggg gagaattcga tcctgctggc gggacaggcc gtacgagggg tcgctctcgg
     1141 ggtggtggtg accgccctgg cccagtcggt gcttgcaggg gtgggcctcg ccatcgcccg
     1201 ggtccccttt gccgccatcc tcacggccgt catgttcatg ctggccatcg cccaggtcgg
     1261 tcccatgctc gtgctgatcc cggcgatcat ctggctctat tggaccagca gcgcgggctg
     1321 gggcacgttc ctgctcatct ggacggtggt ggtgggaacc atggacaact ttctgcgccc
     1381 cgttctcatc aagaaagggg cccatcttcc cctgctcttg atttttgccg gagtggtggg
     1441 cggcctgatg gccttcggtc tcatcggcat cttcgtgggc ccggtcgtcc tggccatcgg
     1501 tcacaccctc ctcctggcat ggatcgacga ggaagcagcc cccacacccc cctctcctcc
     1561 cccggcacag gccacggaag gggggaagcc cgattcgtga caaggccccc ggtctcccgg
     1621 agcggcctgg gtccccccaa tccggaagga accgtcccgg caccgggaca caggcagccc
     1681 acaaggccac gccccggcaa cagcggcgac gaaagcctcg agagcacccg ggtcgagcct
     1741 ttgcggaccg aggcagtcca accctccccg ctaacccttc aggattttgt ccaggatgca
     1801 cttggtgatc aggtaggtcg gtttgactcc ctcggctccc aggctgcccg aagcgagggt
     1861 ttggccgctc atcagcggat tgtccgaagc atagtaggca taccggacct tcacgtcccg
     1921 ggaaatgtgg ccgcggatca tggcggcatt gatggcccgg tggtagtgcc cgccttccat
     1981 ttccaggccc accgccttcc agctggtgct cttgaaggtc tcgaggacgt cccggttctg
     2041 gagagacgtt cccagcacgg tgatcaccgg ccccacaaag acgttgcggt acccttcgaa
     2101 gtcttcttcc ttcagatcgt tctcgatcat gtagttgtgg gcggagccct cgagcacatg
     2161 ggccgtggcg atcatgacgt cccctttctt tcccaggaga gtccccgctt ttcccatgac
     2221 cgacatggaa cgaacgtcga ggtttttttc cacgccgtcc accatcacgg gttgcagaag
     2281 ggaatcgatc aactcaaagg cctgggtgcc aaaggcataa tccatgacga gaatgaccgg
     2341 ctttggctcc cccaccgcgt tcccgtccca tgccaggtcc ggatggaatc cttcccaatt
     2401 cagctttgcc gtatcgatga tctggcaatc cacgtgggcg ccgctccggt ccggcacctc
     2461 ataaaagccg taattctttg cgtattcgag aacttcgttg gtcctgttgc gcaacacgtg
     2521 gaccagggaa tagagatccg gcgggcactc gttggcggcg atccctccaa ccgccccgta
     2581 tccgtacaaa aggttggcaa cgctgtgcag gttcgagctg atgatatgga ggggccggtg
     2641 ctccaggttc aatgcttcca gggtttcccg aatgccggcg gcccatttct tcccgtagac
     2701 ctggtgcagg atcacgtggc tcaggtccgg ggtgaatcgc acctccatat ccgcctgctt
     2761 ttccgtagcg gactccatcc tggttcccag gctgtggacg atttcgaaga ggtcgttggc
     2821 ggctttgtgc tcgtttttca accggccaaa atattcgtag ctgtcccggg tctcatggaa
     2881 agtccggcct agaatgatgc tcaggttcca cagggcacga ttcagcttta cgccgtcgca
     2941 cgggtcccgc tcctccatga atttttggaa ttcctgccat tccgtggtca cgtttccttc
     3001 ctggtcccgc atcttgctgt gaatattctt ggcctcgata tacaggaagg tcaggtgggt
     3061 caggatatcg tagatctcgc tgaagccgcg ggtgatgacg aagcacatct cgctttcgct
     3121 gatgcgatag gaggttctgc ggcgcttgag gggttggagc atcacgaagg gggtgtctcc
     3181 caggtcctct tcggaggtca tcacgaagcg gctgcattct tcgatgccct tgggaagccg
     3241 ttccatgacg tactcgagcc ccttgagctc gatgctcctg ggatcgttca tggttccgta
     3301 gatctcggga ctgagggtcc tcaaggcgtc ctggaggacc tggcccgagc gggcgttcat
     3361 gtgaaagcgc cccatgagca ggagagcatc cgctgcaatc cggaaactgt gaatggccaa
     3421 ccttgcggca ttggatctcg tggtttcttc cataaaccga ccttctgctg ctcccgaacc
     3481 gggggctcca agcgttgaaa cctccctgca accccggggg aagcacggtt ttcctttggg
     3541 cggtcgcgcc gggacgaaag ccccgcaagc cctcgggtcc cccaccctag gaagcaaggg
     3601 tcaaccaggc ttcagggggt gccggtaccc ctagggtccc gaaaaatagg tcacccaggt
     3661 ctgtttgccg ctcgaatcgt acttgacggt ggcaaacccg taatcctccc cggagcccaa
     3721 ctgggtctgt ccggtgacat agacgttccc ggagctgtcc acggcgatgc cggcgggagt
     3781 atccgcaccc tttcctttcc cgtcaaatcg ctgcacccaa tagcgcttgc cgccgggacc
     3841 gtacttgatg gtggcaaaat cggtggtgtt gccggctccc agcgactggc cggtcacata
     3901 ggcatatccc gaggaatcca ctgccacggc cttggtgtag tcgtacccct gacccggtcc
     3961 gttgtaggtc ctcgtccaaa cgagcttgcc ggagctgtcg tatttcaccg tgagaaagtc
     4021 gccgttcccc gtccatccgg tgaggtaggc attgcctttt tgatccgcag caatcccggc
     4081 gggattgtcg gggccgaaac ccgagttgaa gttcttcgcc caggcccgtt tgccggtctt
     4141 cgagtcgtac ttgaggacgg taagatcggt ctcgccgctg ccgccccggg tactgcctcc
     4201 cacgtagacc gaattcttgc tcacggccag tcccacggga tagttgagtt ggctccccga
     4261 ccgggcgtgg cgcttggccc acaactgttt tccggaactg tcgtatttca ccgtggcaaa
     4321 atcataggtc tgctcccccg actgggagat gcccgtaaga taggtgttgc cgctgctgtc
     4381 cacggcgatg ccggagggcc ggtccgaacc cctggtcgga ccgctatatc gtttcgccca
     4441 ctgctgtttg cccgcgctgt cgtagcgcac ggtggcaaaa ctgtcggatc catagtcgtt
     4501 ttccgagctt ccggtaacgt agacgttccc ggaggagtcc acggcaaccc cgttggaccc
     4561 gtcgttgccc ttggcgggcc cgttgtagta gcgcacccac ttctgtttgc ccgcgctgtc
     4621 ataccggatc gtagcgaagt tgctgcttcc cgagccgttc acccactgcc cggtaacata
     4681 ggtgtttccc gcggaatcca ccgcaatccc cgtgggctgg gtaacctcct cccccaacaa
     4741 cccggggccc ccggccggtt gtacggaccc cgcccttgag ggagcctctt gtgcaaggga
     4801 gacacccgct cccaggcccg cagcgtcggg gattgccccc aggcccgcca atcccagaac
     4861 gcccaggagg gttaagaggc aaagcccgcg tcccaacggt ctttcccgaa tcttcatggg
     4921 gtcctccttt ttgttccttc ccgtcctcgg ccctctgccc gtcggaactc cggtgcctcg
     4981 tccgatcgcc cgcgaccccg gtgcagcgga aaggttgcag ataccgggag attccctcta
     5041 cgggaaatac ctcacccagc tttgtccccc gttgggatta taccggatgg tggcgaaggt
     5101 gttcttcccc tgagagctct ccgaccatcc ggtgacatag gtgtatccgt cctggtccac
     5161 ggcgatgtcc gtcggagtgt cgtccccgtt tcccgctccg ttgaaccgcc ggacccagag
     5221 gaccttgccg ccgggagtgt acttgatggt gagataatcg gagccggacc cgtccccttg
     5281 cgactccccc gtcacgtaca cattcccggc atcatccgag cacatggccg tgggacggtc
     5341 gtaatcaatg gtttcggtgt aggaccttgt ccaaccgatg tcccccgacc tattgaactt
     5401 tatggtctgg aacttgcctc ttcccgaggt cccggtaaca tagacgcttc cgttcttgcc
     5461 cgccgcaatg gccgagggat catcatcccc ctgccccgag ttatagtgcc tggcccaggc
     5521 ccttttaccg cttttgcgat tgtacttcac gacggtataa ctcgtattcc cgctcttcgt
     5581 ccgggtcgat ccggcgacat agaccgatcc gccgctctcg accagtccca cagggaagtt
     5641 ataattgctg tccggccggg cgtagcggct cacccaatcg agatgacccg tcttggtgta
     5701 acataccgtc gcaaaatcat aggtgttctc ccccgactgg gagtgccccg tgacgtaaag
     5761 gtagccactg gggtccacgt acagggccgc cgggctgctg gatccttggt agggaccgtt
     5821 gtatcgcttg ttccatactt ccttgccggc gggactgtac cggatcgtcg tgtagtccca
     5881 ccggttctcc gaaacccccg cgtctcccgc cacgtacacg gagccgctgt tgtccactcc
     5941 aaccccgacg ggaaaatcgt aatcctttgc cggaccgttg taacggcgca cccactcttg
     6001 tcggccgtcg ggagcgtact tgacggtggc gaaatccgtg gggccgctgc cgcctgcaga
     6061 ggagccggta acatacgagt tgccggaacc gtccacggcc atcccttggg ggacgtcctc
     6121 gccgccggcc ggaccgttgt agcggcgcac ccacccctgc ttcccttttg aactatactg
     6181 gatggtggta aaatccgtgt ttcccgagct attgggcaag gggccggaga catagactcg
     6241 ctgccgatcc gccacgactt tcggccctcc cccggaaggt ccccccgtgc gcagcaattt
     6301 ggatggggag atcacgggct ccgaggctct cacggtcgcc tgttcctcgg gagcgaccag
     6361 cccggcttcc ctggcggcgg tattgaagct catgcccgga acggccaggc ccacggcgca
     6421 acacagggcc gcaagacgga gtcctctttg catcgatgcc ttttgaattg ccatgagatc
     6481 cccctttaca aaaggttgtc ggtgccttcc tagccggcgg ggtactcccc tactccgctc
     6541 gatccgggac ggccgggcgg caattcatct attgcggatg gttgagttta tttcgatagt
     6601 aataggagtt gctaaaagag gtcaagtagt tccagggagc cctacaagtc tttttttgcg
     6661 actttgtcgg tcctgtcctg tggggggccc caagcgctcg atcccggctt tcggaggggg
     6721 agagacgggc cggactcggg ttctttcgcc ctcacgcccc cccaagaaca ggcaggccgg
     6781 agccaaaacg cggccttcct caggaggaaa ccgcaccggc cgtctctgtg aaaagacctt
     6841 ccgaacgtag gggcgtcttt cgtgggaaaa caagcggaac atcccgggca tcccgccggt
     6901 acgggagccc tttggaaacg gacggtccac tcactgctga tcgttttggt ggccgttttg
     6961 gcctactcga acaccttcga cgtccccttc catttcgacg acacgccaaa catcgttgca
     7021 aactccctga tccgggactt ttcgaacttt ctggaccccg cccgggcccg gggacatccc
     7081 tactacgatg atttcaagat gaggtacgtc gggtttcttt ccttcgcggt aaattataag
     7141 ctccacgggt tgaaggtccc ggggtttcac ggggtcaacc tgctcatcca cgttctaaac
     7201 ggtcttctcg tgtatttcct ggtggttgcc accctgagga ccccgaagat gcggcccgcc
     7261 gtctcgggag acgaaccggt cctctcccgg gagaccggca cgttccccat tcccctgttt
     7321 gcagcgctgc tgttcgcggc ccaccccatc cagacccagg ccgtcaccta catcgttcag
     7381 cgattcgcct ccctggccgc cctcttctac ctggcggctc tccttgccta tgcccgcagc
     7441 aggctgctgt ccgggcaagc ccctcgggcg ttgcgccggg gcctctattt cctgggactc
     7501 ctttgcacca tactggccat gaagaccaag gaacccgcct tcacccttcc cttgagcatc
     7561 accctctatg agctcctttt cttcccggcg ccctccgcaa gcaggtggaa aaggctccgg
     7621 ttgctgctgc cttttgtgct gaccttgggg accattcctc tcagcgccct cgatttgtcc
     7681 ggcgcacacc cgtcctggct ggaccagctg gaacacaaat ccaggattct caccactctt
     7741 tcgagacaag actacttcct cacccagctc acggtaattc ccgtctatct tcgccttctc
     7801 ttcctgcccg cgggccagaa cctggaccac gaccatcccg tggtccgggt tttcctggat
     7861 ccccgcgtgg tcctatcctc gctcctgatc ctttgtgtgc tgctgacggg ggtctacgcc
     7921 ctgcgggcct cccgcttcgg ggacccgcgg tggaggctga cggccttcgg gatcttctgg
     7981 ttcttcctga ccctcctgcc cgagtcgagc attatcccca tcgtggacgt actctacgaa
     8041 caccgactct acctgccgtc cgtgggggcg ttcctctcca tcgcgtccgg actctccctc
     8101 ctcgagggcc gcccgggcag gaatcatccc cgaacccgac tcgggatggt gcttgtggga
     8161 gcggccgtgg ttttcgcctt atcggcggcc acctatgcga gaaacgaggt ctggagggac
     8221 gaggtgacct tgtgggagga cgtggtgagc aagagcccca acaaggaccg accccacaac
     8281 accttggggt ccctgtatct caacagggat cggccggact tggcgtaccg ggagctttgc
     8341 aggaccctcg agatcaaccc ggactactgg gccgcccacg tgaacctcgg gagctggtac
     8401 atgatggaag caacggccgc ggctcgacgc agggaacccg cgaccagggt gttggaacgg
     8461 gtcgaaaaag gctgtcgctt cctacggacg ggccctggcc ctggatccgg gaaacgagat
     8521 gatcctttcc ctccgggagt ccgccctggc gcagcggcgc tccctggttc ccaaggaggg
     8581 gccttgaagc cgctcgcctt gaacctccct cgctccccgg caagagtggt tcccaatctc
     8641 ctcttttccg ccgcggttcg gaagccggga agcatcctcc attgacgatc ctccaagaaa
     8701 tgtgatacgg agaggacatc cccaaacaat ctctcatcgc catcggatgt tcaagctcct
     8761 gcccctaagg cctctcactc atgggctcga cttctgccat gattctggtg gagtaaatgg
     8821 ggtgtttttc ggcagggaaa cccaggttgt cttgctgtgt gtgagaaagg aggaacgtga
     8881 tgagcgtata caaaatcatc gagttggtcg gaacgagtga tcagtcgtgg gaggaggcgg
     8941 cgagaaccgc tgtggaaacg gccagtaaga gtctcaggga tttgagggtc gccgaagtag
     9001 ccgagatgga cttgaaattc gaggaaggca agattctttt tcgcgtgaag ctcaaggttt
     9061 ccttcaagta cgagggacgc agctaatcaa agtccgtccg agggtctgtt ttggccgggc
     9121 aaacccaaga actcacgacc aaggcaccgc aaaccgacct cttccggcaa gcgcggcaaa
     9181 tctacctgtt cccggaaggc atgctgcctt tttcttccag cgtgccttcc gaccctggac
     9241 agccaaatcc aacggtttgg attccggggg ggatcgattc gggttcgcgc aaagcccgtc
     9301 gccacaaagg tccggcgagc tcacccggca ggggcgggat tcccttcccc gcggtgagga
     9361 accgggccct tccgcagccc gcgcaaggga attccccgct ttgaacccct ccgcttctcc
     9421 ttcccccccc gagactcgtg gcaaagatgg cctatttttg tgatttggac gacaatcgag
     9481 ggacttttcc gcgagtctcc gatatcattt tatgtttcct ttggcgggat tgagggaccc
     9541 cgcccggggg tgaggacggg ggccgatgcc ttcctgaacc ctttccagct gtcggccgat
     9601 gacagctttg ttccttcggt gcggcgaagg atccgccgaa ccctgggaag caccctttct
     9661 gccccgggtt atggacgctt tgttcacggg acggttacgc aaacccagtg atttcaggag
     9721 gattgcacga tgattcgtag ggaagatgcc ctgcagtacc acatccagga acgcccgggg
     9781 aagatcgagg tcaagtcttc gaagccctgc atcacccaga gggacctttc catggcctac
     9841 actccggggg tggccgagcc ctgcagggaa atcaataaag atgcggaccg ggttttcgac
     9901 tatacggtca ggggcaacct ggttgcggtg gtgaccaacg gcacggcggt gctggggctg
     9961 ggcaacatcg gacctcttgc cgccaagccg gtcatggaag gcaaggcggt gcttttcaaa
    10021 cgttttgcgg acatcgatgt gttcgacatc gagctggggt ctacggatcc cgaggaaatc
    10081 atccgggccg tcaaactgct cgaacccact ttcggcggaa tcaacctcga agacatcaag
    10141 gccccggaat gtttctacat tgaggaacga ctcaaggaga tcatgaccat cccggtcttc
    10201 catgacgatc agcacggcac ggccatcatt tccggcgccg ccctcctgaa cgccctcgag
    10261 cttcagcgca agcccatcga ccaggtaaaa atcgtgtttt ccggggccgg ggccgccggg
    10321 attgcctgtg ccgaactcta catcaagctg ggcgtaaaac gggaaaacgt gttcatgacg
    10381 gattcccacg ggctcgtcca ccacgggcgg gaacagggga tgaatccgta caaggagcgc
    10441 ctggccaacg ggtcccggcc catgacgctg gccgaggtcc tgaaggacgc cgatgtgttc
    10501 gccggggttt ccgcccccgg ggtactcacc ccggagatga tcaggagcat ggccccgcgg
    10561 cccatcgttt tcgccttggc caatcctgac ccggagatcg gataccatga ggccgtccgg
    10621 gtgagggacg atctgatcat ggccacgggc cgttccgact atcccaacca ggtgaacaac
    10681 gtgctggggt ttcccttcat ttttcgcgga gccctcgatg tacgggccac ggccatcaac
    10741 gacgagatga agattgccgc cgtcaacgcc cttgccgacc tcgccaagga ggacattccg
    10801 gattcggtcc tcaaggccta cggaggccag gtactccggt tcgggcccga gtacatcctt
    10861 cccaagccgt tcgatccccg ggtgctggtg tgggaggccg tggccgtcgc caaggccgcg
    10921 gtggcctccg gggtcgcccg aaagcccatc tacgactgga acgaataccg ggatgccttg
    10981 gagagccgcc tgagccgctc ccaggaaatc atgcgcagaa tcatccaccg ggcccaaagg
    11041 gaacccaagc gcatcgtttt cctcgagggc aacgagccga agatcctccg agcctgccaa
    11101 gtcatcctgg acgaaggcat cgcccggccg atccttctgg gagaaagaag cgtcatcgaa
    11161 cggaacatcg tcgaattggg gctcgaactc gataaggccg taattgtcga cccccggaac
    11221 tttgaacgct tcgacgaata cgtcaccgaa ttccaccggt tgcgctcccg caagggcatc
    11281 acccgcaaag aggccaccaa gctcctgacg gtcaaccgca attacttcgg ggccatgatg
    11341 gtccggatga gggacgccga cggtctggtg ggcggaatca acaagcacta cccggaaacc
    11401 attcggcctg ccctgcaggt cctgcccctc gaggaaaaga cttccgtcat cgcgggcatg
    11461 tacatgatgg tctttcccaa agacgtgatg ttctttgcgg acaccaccgt aaacatcgag
    11521 cccaccaccg agcagctggc ggagatcgcc atttgtgccg ccgacacggt gattcgcctc
    11581 ctggagatca agccgcggat cgccatgctt tccttcagca acttcgggag cacccggcac
    11641 ccgctgacac aaaaggtggc cgaggccgtg aaggtcgtga agaaacgccg acccgacctc
    11701 aagatcgacg gcgagatgca ggccgacacc gccgtgattc cggagatcct gaaccgggtt
    11761 tatgacttca acgaactgaa aggcaaggca aatgttctca tctttccttg cctggatgcg
    11821 ggcaacatcg cctacaagtt gatggaacgt ctcggcggcg ccagggccat cggccccatt
    11881 ctcatgggaa ccagcagggc cattcacgtg atccagcgaa actgcgacgt cgaggacatc
    11941 gtgaacgcga cttccctggc cgtcatcgac gcccaggaac actgagggca ggtcgtcaca
    12001 gggagagcct tcgagggggc tgcggtattt ggaagtcacg ccatatctcg atagaaactc
    12061 ggcacttgac ggggtatcgc ggggcaggaa cagacgattc cccggggacc taccggggcc
    12121 tcttgagtct cttcttcatg gtgagggtgt tgacgccggc ggcggcggtg tatcggacct
    12181 catccatgac ggtgcggatg atgtaaaggc cgtagccgcc ttccgggtgt cggtcgaatt
    12241 ccggaagagg gaccttctcg atgtcgaacc cttctccccg atcactgacc tcgatgacca
    12301 gctccccctc gtagactcga aagcgaagga ccacccgggc tgtccccccg gcccgggccg
    12361 agtgctttat ggcgttggta caggcctcgc tcaccgccag ttccacgtcg tcgacaagac
    12421 tgcccggtag atcttcccgg gcggcggctt cgctcaccag ccgcgacacc gccgtggcca
    12481 ggcgcaggta gtccagtttg cgttgaaatt ccagggtcag ctcgaaccac ggcgtgctca
    12541 tgagacggta cccgtcccgg cgtcccgaaa gctgctcacg gcagtccccg cactgtcgaa
    12601 gacctcaaag atatgatgca gacggatgag ctcgaaaacg gccctcaccc ggctttgcgg
    12661 agccgcaatc cggatatctc ctcccagctt gttcacggac cgcaggcacg ccaccaggct
    12721 gcccaacccc gagctgtcca cgaaatccac cccggccatg tcgatcacca ggtggatccg
    12781 tcccttcttg gcacaatcct tcaccttatc cttcaggctc cccgccgatg cggaatcgag
    12841 gcgcccgttg agcttcaata ccgttgcctt gccgacgatc tcatccaaga tctccatatc
    12901 tgcattctcc tttcgtggtc aggaaagcgg cagtgcggcc gggaatccgg acgacgaccc
    12961 gggtcccggt ccccaaggtg ctttcgactt cgagggagcc gccgtgctcg gcaatgattc
    13021 gccgggcccg gcacagcccc atccccaccc cgaccgcctt ggtcgtaaaa aagggatcga
    13081 agacatacgg caagtctctt tcggcaatcc ctccgccgtt gtcccgaatt gcaacgacca
    13141 gcatttcccc ttgccgaacc aggtcgattt cgatccttcc gccccccggg ttcacggatt
    13201 ccacggcatt gtcgaggacc tccccgaggg cttccgccag gagatcgggg tccatctcga
    13261 cttcgaggga atctgcgtgg atggtgagct cgacctcccg ggagcactct gctgactttt
    13321 ccatgacccg ccgccgcatg gattcacgga ggacgtcgac ggggacccgc cggagggaca
    13381 cgggaggcag ggaagcgtaa tcatggacgc tgcgcaccag gttttccagg cgccgggtgg
    13441 agttgaggat ctgttgaagg tactcggcct ggagactgcc gggctccgcc ttcttgagca
    13501 tgagggccgc aaaacccccg atggcggtgg tgggattgcg cagctggtgt gccacggcca
    13561 tggccagctt gttcaggctt tccgtccgtt ccatctggag ccggttcctg tcctcgagaa
    13621 tcttcttttc ctggaggtgc agcgcgtgga gctccgtcac gtcgtgcagg aggaccacga
    13681 tgcccgccag tttctcgtcc cggcgaatgt aagaggtggc gatggagagc tgcaggaccc
    13741 cgccgtcggc acgaacgtaa ggcaccgttc ggcgcaggtt cagccgttct tcccagatca
    13801 cgtcgacgat gacctgggtg aaagaatcgt tttcttcgca tagaaacagg tccgcccaac
    13861 cctttccggc caggacctgg ccgggacggc cgagaatcgc cgacgccgcg ctgtttgcca
    13921 gaatgatttc cccccgggga tcgattacca ggagccccac ggcaaggttt tcaaaaatgt
    13981 tttcgatgat gacagggtcc acgtgccttc atcctaggtc aaaattctct ctacggcagg
    14041 cagggtcatg tcgtctccga actcgtcgcc ggcctccacg acgagccgtt tctgatgtcg
    14101 cctatagcat agcacggcgc tgatccggtg aaagaggcga aatgccttta ccagcaggct
    14161 tttttttgcc ggtgtgataa ggcggagacc gatgaaaacc agggttgtcc ccctacgaaa
    14221 tttccggagc acaggagcga gggaagagcc gtgttttccg ccgtgaggaa gtaggactcc
    14281 cgcccgggca attcccctcg aaatttcctc gaaagcggac aaacgctccg gaaagaagaa
    14341 gagccgtcag cccccgccgg atcaaaaggg gcccgaacgg cgtccgccgg tctcaaaggc
    14401 gggaatgttc aaaaggtttc cgtcatggcc tggactactc cgcagaggga gacccaaggg
    14461 tcgacaaaac aaagagcaac acatccccgg attaagcgat ttggccgcca cgaatcccgt
    14521 ttcgtcattc ccgcgccgtc gggaatccac gaaaagcgcg catacccacc gggtcccctc
    14581 cgtcgccggc atgacgggtt gatgctcccg aaatcgatca actcaggaca tcgtctcgaa
    14641 gagggtcgag gacccgcatt ggccggcagg ataagcttct tgaccgctca gagcgcatgt
    14701 gctaggcgtg atgctccagg agagattttc cctcgctccg cttctcccta gcccgaacgg
    14761 aacggtcggc acttcccggg gatcttgaac ccggtctcca ggcgatcatg tctccgggac
    14821 ggggtcgggg tgagaggaaa cttccccatt tccgggcaag tcaaacggcc cggggctttc
    14881 atggacatcc cgggaacgaa gcacggcagg acacaccatg aggccgacgg cccggggctc
    14941 cccggacatc cccaaccgcc cacacggaga cgcgagatgt cgccctatga acctcacaac
    15001 ggcctgactt cctcttccga agcccgtcgc acccggcgag ccgtcgagat cgcccgggcc
    15061 gtttttcccc aagcggcggc ccgggccagg agcaccaagc ccgaaaatct ttccaacgcg
    15121 atccgaaacc tggacgagtg gttgagccgg gatcgtttcg cggactatcg cgaggccctt
    15181 ctcgaccttc tcgagatcgc ggcgcaggac ccggccgttg ccgatgaact ctacgacagc
    15241 ttctaccgcg tcatgccctt cggcaccgga ggcaggcgat ccaaggtagg aatcggaccc
    15301 aaccggatga acgcctacat ggccgcaatg acggcccagg gcaacgccga gttcatcctc
    15361 gggcaactgg cccggcaagg gacctccgtt tccgaaaccg acctcttcgc gggggcctgg
    15421 gacgtccggg aattccaccg atattttgcg gaaaccccgg ccctcgaacg cattcgaaag
    15481 gtcatcgagg acaagtgccc ggccctctcg ggactctcct cccgggacct ctcggaaatc
    15541 gcggggctgg tgtacgcggg caatcacatt cgctacgtcc accccgtcgc catgcgcccg
    15601 accccgtggc tctcctttct catcaatgcc tggggacgga tcgtcgcccg gggccccttt
    15661 tccggggaag ccgccgtttt gaaccggtgc cggcgggtct tgggcggcat cgtcctgagc
    15721 tcgagccaca acccttacga caacaacggg acgaagtttt acgaaatgag cggcgcccag
    15781 accccgccgc acatcgtcga ggtgctgcag cgcatcgggg atgccgtcca agaaatccgc
    15841 tacttcggcg gggacactca ttcccggcaa ggccgcaagg ccgccttcga ggccgcgagg
    15901 aaaagcggca tgatcgtggt tctggacgag gaaaatctcg agcagatcga ccggttctac
    15961 gttcagaact ccatcgaaga gattcaaagc ctgtatccgc cccaggagtg gaaggcgctc
    16021 gccacgccca gcaaccccaa cgatccctat tctccggcct tcgggcggct catcgtctct
    16081 ttcaatgccc tgaacggcac cgggagcgtc ggcgttctca agatcctcga gagcgccggg
    16141 tttcccgtcc tgcgcagccc cggggacctt cccacctggg aatttaccga ggggtacggc
    16201 aatgtcccca atcccgaggc ggaaaagacc ttcaacacgg gcatgcagat cggggtccgg
    16261 cggagcctgg cggccctgct ctcgggagat cttcgcggcc tggcgtccgt ctcgagcctc
    16321 atcgacgagg acggcaaccg ggtgccttac gcggcgtttt ccgcggcagg gagcgccgac
    16381 gaagcaatcc ggtccttccg agaggccacc ggcaaggccg tccggggtct cgagctcgcc
    16441 ccctccgact cctcggaaag caccctcgcc tacctcaagg atcacgtgct tcgaaacaac
    16501 atctgcctgc tgaccgaccc ggacgccgac cggatcggac tgggcatgca ggtcttccgg
    16561 gtctccgaag accgcaggtc cgtgaggttc cagtggatct cggccaacga caacgacgaa
    16621 tcgggcatcg tgcttttccg gtaccggctc gaacggatgg tggagatggc ccggcgcggg
    16681 gaactcatcc ggttcatcga agaccggcgc cgggaagacg gctgccccgc cgaacccggt
    16741 cagaaccacc aactggtggt ggtcaacacc gtggtgtcga accccctcga ggccgtcatc
    16801 gccgaaagga tttcctccca actggaggaa ctcaccggcg gcagggtttc cgtcaaggca
    16861 attacccacc atgtgggctt caagttcacc ggcgagatca tcgacaacat caagcggggg
    16921 aatgccggcc tgcccttcga agggatcacc ggggaactga tgaccagggc gggaatccgc
    16981 ggcagggagg ccttctttgt cctctcgtcc gaagaagggg aagggtccct gatcggctac
    17041 cggggcagca tcgacaagga cagcggcgtg acggggctgg ccctggcggt cctggccgcc
    17101 gaacagcaga accggggcat gaccctctac gaatacctga tggagaccta cgaccgttac
    17161 ggctactgca aggccgccct cgaacccatg gtcctgacgg gcgagtacgg aatgaccatg
    17221 atccacgacc atatcatggg gcgtatgcgc gacgagctgt tgcccgcggt ccgggggggg
    17281 gcgcggccgg aatggcccct gccggacggc tccgaccgcc tggtcctcgt gggcgggacg
    17341 gatcacctgg atctcatgaa aaaatccccc gacgaggacc ccgcctcatg gactcccacc
    17401 cggcgcaacc tccccgacga tcctgcccgg tggcccgccg ccattcgcga gtccatcaac
    17461 atcctcgagt tctacgcc
//
LOCUS       DAOOVC010000078        17450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2234_length_17450_cov_6.626444, whole genome shotgun sequence.
ACCESSION   DAOOVC010000078 DAOOVC010000000
VERSION     DAOOVC010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17450
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2234_length_17450_cov_6.626444"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /gene="waaF"
                     /locus_tag="PLM79_10830"
     CDS             <1..1112
                     /gene="waaF"
                     /locus_tag="PLM79_10830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOV86848.1"
                     /translation="PEERRFFESVWTRWSGAHPRGKSPRGFKTILVRCPNWVGDVVMA
                     TPAFDCIRQNFPHARIVALVRRYARGVLEDGPWFDEILDCEDRSPGGFLRTVQTIRRQ
                     KPDLAVILPNSVRSALAARLAGVPRIAGYRRNGRSLLLTSGPEPRRTSGKILPIPMVE
                     YYLDLCRWMGWKVPTDPKPSLFMSPGLEEKAEGILREHGILPGDRIIGINPGARFGSS
                     KCWPPEYFARLADLFALEGNGKLLLLVGPGEGDIARAILRATRAPILDTSPYKVDLAL
                     LKPLVSRCSLMITNDTGPRHYAVALGVPAVVIMGPTDPRYTAANLENTLVLREDLDCS
                     PCHKKVCPTDHRCMTRITPETVYRAGVELLKSREP"
     gene            1109..2599
                     /locus_tag="PLM79_10835"
     CDS             1109..2599
                     /locus_tag="PLM79_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOV86849.1"
                     /translation="MTAISYRDRWVSFRNHGRDLEELSRLASQIALSFMDHYYQNGRF
                     EEESIRLLCEMATHFEDPRWNRAASSALFEIIVEGLCDDFEEFQPETYNRVMSEVIDF
                     CRRLPEGKELDEALRDFGIATDRDLLARLERTRSTRAYPGISRQPRKVLVLSRVTIGA
                     DVAITSVILQRLETIFPGAEFVLLGSPRLREIFGGHRNLRVREVEYVRRGGLFERFSA
                     WHEALAAVREETRQTSPDHCLLVDPDSRFSQLGVLPLTRNHDCLFFDSRGSASYLSRM
                     NMAELANHWLNGVFGVSDFRYPRVWLPEGTLEAAHSACSAIRACGCRHLIAVNFGVGG
                     NWRKRAGPDFERKLLERLLENPDTVILLDKGSGDNEEAQVRVLLEALRSSNHSCEETR
                     FGSLGEKPLSHGVIGVQCSIGEMGALISRCDEFIGYDSACQHISASLGIPTYTVFAGS
                     NNTRFIRRWSACGPARRRIIHVDTLSSPGPLDVEGIISRIMDARSQ"
     gene            2596..4116
                     /locus_tag="PLM79_10840"
     CDS             2596..4116
                     /locus_tag="PLM79_10840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86850.1"
                     /translation="MKILFLYPNANSQLGFNYGVAHMAAVLKEAGHSVSFRQLCDEMG
                     PLPSREEFIQFVREAAPDLIGFSVVTNQWGYARTLARWAREAVSVPLVIGGIHATVAA
                     REILETGLFDYVFRGECEEAFLEFVEKFQRGEDLHDLSNLGLVYRGSVRINPLRRLPD
                     LTALPPKDYGILDFQEMIDAKGGWVGLMGSRGCPFSCTYCFNHLMVQQYRKDLGCGFR
                     DLGYIRHFRVDQIIAEIQYLTANYRNIRMFIFDDDLFTFRKGFVKEFCREYKRVSDIP
                     FVVNAHVGFFDEDRARALADANCRIVKFGVESGSERIRRRIMNRHTSNEKILSSIRIA
                     RNFGLHASIFLMIGLPFETREDVMETVRLMATAKPGRFRWSFFFPFPGTRAHELSLQG
                     GYIDLEKMNTLMNFTDSSCLKFGEEHDLFLEKVGRVFPWFVNAHSDLPVRDYYRRRVE
                     DLLALSASEWEKASPGLIEEDGVLSEAFAQKGLSHYAVKYNRFMGVISDYFLQEGA"
     gene            4279..4785
                     /locus_tag="PLM79_10845"
     CDS             4279..4785
                     /locus_tag="PLM79_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HOV86851.1"
                     /translation="MENKLLQLAVQIVHAQASHAKMSGEEIEAVLMKVYNSLYKIEQA
                     EAEGRPLLPEELAVAPTPGGEPAFRSEPRSSIHDDKVYCLECGAEFRQLTANHLRMHR
                     LTPREYKRKWGFPLKQPLAARILTSLRSRSAKKRGLPVKLKEYLEDRKKRKGYGPDRG
                     SVSHSASG"
     gene            complement(4872..5405)
                     /locus_tag="PLM79_10850"
     CDS             complement(4872..5405)
                     /locus_tag="PLM79_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86852.1"
                     /translation="MTARTEESSQYRRPMEDEEWRSVVLELRRGLARLDSRQVQARVL
                     GIRQGLNCLDAMMSRYCELTCGSCPDRCCHGKNIFYNRADLLFLASSGLDLPPGQTRA
                     RHGEPCRYLGERGCDLDRARRPYVCVWFLCEPQVKLFRQEPPHFQRQALKWMESIRRS
                     RLEIESLFERASRKDGR"
     gene            complement(5445..6098)
                     /locus_tag="PLM79_10855"
     CDS             complement(5445..6098)
                     /locus_tag="PLM79_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HOV86853.1"
                     /translation="MTGKEQLLLEIYHRLLDEFGPQHWWPADTPLEVIVGAILTQNTS
                     WKNVEQAVRVLKDNELLDLHALGEVPVGQLASLIRSAGYYNQKAQKLKAFVRWVEEHW
                     QGDLGRFLGQETAQLREELLGVRGIGPETADSILLYAANQPSFVVDAYTHRIFSRHGW
                     VQEQISYWELQEFFMSNLQPDTALFQEYHALLVRTGHLFCRRKPRCEPCPLRGWKGE"
     gene            complement(6115..7128)
                     /gene="amrS"
                     /locus_tag="PLM79_10860"
     CDS             complement(6115..7128)
                     /gene="amrS"
                     /locus_tag="PLM79_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOV86854.1"
                     /translation="MREARWWRRVDESLVHCFLCSQQCRIPPNERGKCGVRENREGTL
                     WSLVYGRVIARNVDPIEKKPLYHFHPGSRSYSIATVGCNFRCLFCQNADISQAPVEYG
                     NIWGPEIPPHEIVEQARRTRCTSISYTYTEPTIFMEYALDVAEPAHRAGIKNVFVTNG
                     YLSRESLKSLAPFLDAANVDLKAFDDDFYGKQCGARLKPVLKTLEGLKEAGIWFEVTT
                     LLIPGLNDGEESLRRLAGFLAALDPSIPWHISRFHPMYHMTDRPVTGVAALHRAREIG
                     IEAGLRYVYVGNVPGDDGENTYCHRCGNLLIDRFGFTMRIRGLREGKCEQCSTVLAGV
                     GIS"
     gene            complement(7125..7985)
                     /locus_tag="PLM79_10865"
     CDS             complement(7125..7985)
                     /locus_tag="PLM79_10865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV86855.1"
                     /translation="MGYTFGYEDSLRYRDWFRSEPGRTALAVEKELLARLWAPSCPQR
                     VLEVGCGTGVFSEWFAQQGHRVTGIDPSPYMLQIARSQLPWNIHFHKGCAEHLPFDDN
                     AFDMVALITTLEFVEDPVTALTEALRVARRTVLLGVLNRYSLITLERWIEACWRPTVY
                     RYARFFSIPDLRHMMEEILRGPVRLRWRTCLSLPLGALKYTHRLERSRWMQWLPLGHF
                     IGMRLDLTYPLQTVQQPLFAELPSAVGHGGLRSACWYSSFKKRSNSAGPRSQAAYGDE
                     GGPLWKGGGA"
     gene            complement(8190..9851)
                     /gene="ilvD"
                     /locus_tag="PLM79_10870"
     CDS             complement(8190..9851)
                     /gene="ilvD"
                     /locus_tag="PLM79_10870"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOV86856.1"
                     /translation="MRSDIMKKGIEKAPHRSLFRAMGYTQAELDRPIIGIANSVNEII
                     PGHIHLDKITEAVKAGVRHAGGTPVEFGTIGVCDGIAMNHDGMRYALASRELIADSVE
                     VMAMAHPFDGLVLVPNCDKIIPGMLMAALRLNIPSVMVSGGPMLSGRFRGNPVDLVSV
                     FEGVGSYKTGQMSAEELEELEENACPGCGSCAGMFTANSMNCLTEALGLGLPGNGTIP
                     AVTAARIRLAKLAGMKIIELVSRNLRPRDIATRNAFENAMAVDMALGCSTNTVLHVPA
                     IANEAGIELDLDLFNTVSARTPHLCSLRPGGAHFLEDLDAAGGVQGIMKVLADAHMIH
                     LDGMTVTGETVGANLRKVRKIDHEVIRPLDNPYHKEGGIGILYGNLAPEGAVVKQSAV
                     DPEMLCRKGRARVFASESAAASAILGGAVVPGDVVVIRYEGPKGGPGMQEMLTPTAAI
                     IGMGLGKDVALITDGRFSGGTKGAAIGHISPEAAEGGPIALVEEGDEILIDIPNKKLE
                     LLVDETVLKQRREKWTPPEPRVKEGYLARYAQMVTSGSRGAVLRR"
     gene            10073..10510
                     /locus_tag="PLM79_10875"
     CDS             10073..10510
                     /locus_tag="PLM79_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOV86857.1"
                     /translation="MDDENRKALYPIGIVAELLSIHPRTLRIYEQEGLIRPARRGGKR
                     FYSNNDLQWLKCLRKLLSDEGLNIAGIKKLLTLAPCWKIRGCPEEIRKQCPAILNFPV
                     PCWDLVPRECKENSQSCSDCEVYLNKMNHVMMQRYKEEPSPEK"
     gene            10533..11468
                     /locus_tag="PLM79_10880"
     CDS             10533..11468
                     /locus_tag="PLM79_10880"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86858.1"
                     /translation="MPTIFGPVPSRRLGRSLGIDVVPHKTCTYNCVYCEAGPTTCLTL
                     DRQAFLDPDRVLGELADYFRNHPGSVDVLTFAGAGEPTLYLPLGDLIREIKKRYPSYP
                     LMVLTNGSLLWDPSVRRDLMEADRVVPSLDATIEEVFRRVNRPHPRLKLSRILEGLFA
                     FRQEYPRPLHVEVALVRGVNESRAALENLRDILDALDPPRVELNTVVRPPAVPGVMGL
                     EPHEMEKARRILGRGRTEIIGSFLHPPVVGSDANLEERILAMIRRRPCDPQEMARSLA
                     VEPHELESASRRLRKKGVVSKQRFGDREYLVPSEK"
     gene            complement(11511..14231)
                     /locus_tag="PLM79_10885"
     CDS             complement(11511..14231)
                     /locus_tag="PLM79_10885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOV86859.1"
                     /translation="MGKGTVVGGALLVLVFGLVGPVFSSGDRLSIPSIPDTLRPFVQQ
                     VLSSQPGDVFVVLKNGLTVLVHPKLQNDVVSAQVLVRAGLIYEGKYRGSGISHYLEHV
                     LSGGSTRSFTEAQAKERLQAIGGESNAFTSYDRTGYYINSSSKHWKDSLDLLISYVTE
                     SILDPQEVAREKSVIQQEIKMGENNPGSELWKLYMNTAYRNSPVRDPIIGYEEVFLKL
                     DRDALEDYYSRRYHPENMVVVVSGRVDPWKVIEFVYTKTKDFRRKVETALPGTTEPPQ
                     VSPRRQERELPIVRLTQAILGFPSVDLHHPDLYALDVLALLLGQGESCNLVCRLKDRE
                     NQVLSVSATNWTPSYVHGQFMVNVTLPPQNWPQAISIIHDEMDRFKREPVKPKELEKA
                     KKSIIAQHICGKESSHARGSSLAVSYLETGDPYFEETYVDGIRRVTAEEVRRVARRYL
                     DRGRMNVAATHPPGGEKAAQSAPVAPATAADDPPVRFAELPNGLRTLLKRDASLPRVT
                     IQLYGLGGLSLEGSQRPGISAFTASLLTAGTGKRNKMDILRAIEDVGGTIESKSDNNS
                     YHIAVKVLKEDLDVALDIVADLVRNARFPESEIEKKRTETLMALRRLDENWQSEVLRL
                     FKRNYFQQSSYGQDRLGTEESVGSFSRREILSFYRKMVNPRHSVLAVYGDLDVEKTSR
                     TVREKFQGWRGVQATLPPWPDETRPLTADRLVEKKNEKTSAALFIGTNGLAVDSEDRP
                     ILDVLDAVLSGRGSPCGRLFDALRGKEDLVYVVSGFPFYGRKAGYFGVLTQTTPSNLE
                     KVQEIVLENLRKMAREPISPEDLQSAKDTIHTSQALQMESLDAQARTAALNEVLGLGW
                     DHEDRYMQRVQAVTAEEVQQLARELFSNTLIVRTIPQAAGETPPSTARLK"
     gene            complement(14447..15211)
                     /locus_tag="PLM79_10890"
     CDS             complement(14447..15211)
                     /locus_tag="PLM79_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86860.1"
                     /translation="MEHSRVALAPFLADCPEVLTLGVRPGFEDYSAEERQTLRKASRI
                     FFPTPRFVSVFHAAGLPCFPSYYSYRYQRSRLLQNALFQWVGIPHLGGRAYYGPRQKE
                     AILRDFPTPFLLTGPFARPDTVHVVNCGEDLRGLRDRYNPVLVRPLVSWRRQVRLVFL
                     RHGCIGAQVLTASRCGTSTWEPVSTGEKNLDSVIRQTRGLLRGAQIDDILVHWGHDGE
                     SWKLIEMLRPPLRVQTPSGVVRRHQLVGRLIRESIL"
     gene            complement(15274..16704)
                     /locus_tag="PLM79_10895"
     CDS             complement(15274..16704)
                     /locus_tag="PLM79_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86861.1"
                     /translation="MFVKTLNIVLAEWIKIPSDGLSPLLERKIHERLVFTNPDYEARH
                     NRGEWIGNIPAQISCIRQKGRHYLLPRGFLDQLQELCKKYQQPYRIVDKRRSFEPLSL
                     EFHGELKSYQQEAAEAILEREFATLIGGHKSGKTVIALYAIAQRRQPTLILIPKLDLL
                     DGWLTKISNFLQIPGSEVGVFSGGVHQIGNTITVAHTGEVMRHWRKLWDRIGFLIVDE
                     CQRCPSKVLTYLIPNFDCRYMLGLSNTAQRKDRLSRLVYYHLGDVVYSINEKDAREGR
                     GIIHAHVVARPTPFEYPYHSRSDYQPMLEALARDEGRTRLIADDIESELREVPQPLLV
                     LTTETVGQGALEAELGKRGIPVMPLKVESLAGEEDVEEPPLPDACPENVEWPTGPVAV
                     LVTPNTLAQCSANLKARVLFLAVPIYFRKHLANALRNLYVNGNDQKLKIYDYVDHRIS
                     LLENYFRMRSYNYGVHPDVLLNSGPL"
     gene            17167..>17450
                     /locus_tag="PLM79_10900"
     CDS             17167..>17450
                     /locus_tag="PLM79_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86862.1"
                     /translation="MEDQSSDHLLILKQLALEYQQKYGELEQFVRNTEPQRLLIQLRN
                     QGELATSRFRSFEVALLMALSQRAGEQDGDATLRLATDLCRCFDEMRILF"
BASE COUNT         3405 a         5322 c         5215 g         3508 t
ORIGIN      
        1 cccccgagga acgtcggttt ttcgagtccg tttggactcg atggtcgggg gctcatccac
       61 gaggcaaaag cccccggggg ttcaagacca tcctggtgcg ctgtcccaat tgggtcggcg
      121 acgtggtcat ggccactccg gcctttgatt gcatccggca gaatttcccc catgcgcgta
      181 ttgttgcctt ggtgcgcagg tacgcccggg gagtcctgga agacggtccc tggttcgatg
      241 agatcctcga ttgcgaagat cgatctccgg ggggcttttt gcggacggtg caaaccatcc
      301 ggaggcaaaa gccggacctg gccgtaatcc tgcccaactc cgttcgatcc gccctcgcgg
      361 cccgccttgc cggggtcccg cggattgcgg gctatcgacg caacgggagg agcctgctcc
      421 tgacctcagg tcccgagccc cgaaggacct ccgggaaaat cctgcccatc cccatggtcg
      481 agtattacct ggatctatgc cgctggatgg ggtggaaggt gcccacagac cccaaaccca
      541 gcctgtttat gtcgcccggc ctcgaggaga aggcggaggg aatcctccgg gaacacggaa
      601 tcctgccggg agaccggatc atcgggatca atcccggcgc ccggtttggt tcgtccaagt
      661 gctggccgcc cgagtacttc gcccgactgg cggacctgtt tgccctcgaa ggcaatggaa
      721 agctcctgct gttggtgggt cccggggagg gcgacatcgc ccgggccatc ctccgtgcga
      781 cccgggcccc gatcctggac acgagcccct acaaggtgga tctcgctctc ctcaaaccct
      841 tggtttcccg gtgttccctg atgatcacca acgataccgg tccccggcac tacgccgtcg
      901 ccttgggggt cccggccgtg gtgatcatgg gacccacgga cccgagatac accgccgcaa
      961 acctggaaaa caccctcgtc ttgcgggaag acctggattg ctccccatgc cacaagaaag
     1021 tctgccccac ggaccaccgg tgcatgaccc gaatcacccc cgaaacggtt taccgggcgg
     1081 gggtggaact cctgaagagc agagagccat gaccgccatc tcctaccggg accgatgggt
     1141 ctccttccgg aaccatggac gagatctcga agaattgagc cgcctggcat cccagatcgc
     1201 tctttccttc atggatcact actatcaaaa cggtcgtttc gaagaggagt ccattcgcct
     1261 tctttgcgaa atggcgaccc atttcgagga cccccggtgg aacagggcgg cctcttcggc
     1321 tctctttgaa atcatcgtgg aagggttgtg cgacgacttc gaagaattcc agcccgagac
     1381 ctacaaccgg gtgatgagcg aggtgattga tttctgccgc cgacttcccg agggaaagga
     1441 actcgatgag gccctccggg atttcggcat cgccaccgat cgggacctcc ttgcccgact
     1501 ggaacgcacc cgctcgaccc gggcttatcc gggaatcagt cgtcaacccc ggaaggtcct
     1561 ggttctttcg agggtcacca tcggtgcgga cgtggccatc accagcgtca tcctgcagcg
     1621 cctggagacg atctttcccg gggcggaatt tgtgctcctg ggaagtcccc ggctgcggga
     1681 gattttcgga gggcacagga acctgagggt gagagaggtc gagtatgtga ggcggggagg
     1741 actcttcgag cgattctccg cctggcatga ggccctggcc gccgttcgcg aggaaacccg
     1801 acagacctcc ccggaccact gcctcctggt ggaccccgac tcccgcttct ctcaactggg
     1861 cgtgttgccc ctcacccgga atcacgactg cctgtttttc gacagccgcg ggagcgcttc
     1921 ctacctgagc cgaatgaaca tggcggaact ggccaaccac tggctcaacg gggtcttcgg
     1981 ggtttccgat ttccgttacc cccgggtgtg gcttcccgag gggaccctcg aggcggccca
     2041 ctccgcgtgc tccgccatcc gagcctgcgg gtgccgacat ttgatcgccg tcaatttcgg
     2101 agtgggaggc aactggcgaa agagggccgg gccggatttc gagaggaagc tgttggagcg
     2161 tctcctggaa aaccccgaca cggtcatcct cctggacaag ggctccgggg acaacgagga
     2221 agcccaggtc cgggttctcc tcgaagccct gcgctcctcg aatcattcat gcgaggaaac
     2281 ccggttcggc tccctggggg aaaagcccct ctcccacggg gtcatcgggg tccaatgttc
     2341 catcggggaa atgggggctt tgatctcccg gtgcgacgag ttcatcgggt acgactccgc
     2401 ctgccagcat atttccgcct ccctgggaat tcccacctac acggtatttg ccgggtccaa
     2461 caacacccga ttcatccgca ggtggagtgc atgcggaccg gcccggcgca ggatcattca
     2521 cgtggatacc ctctcgagtc ccggtcccct cgatgtggag ggaatcatct cccgaatcat
     2581 ggatgccaga agccaatgaa gattctcttt ttatacccca acgccaactc gcaactgggc
     2641 ttcaactacg gcgtggccca catggccgcc gttctcaagg aggccggaca ctcggtgtcg
     2701 ttccggcaac tgtgcgacga gatgggtccc ctgccctccc gggaagaatt cattcaattc
     2761 gtccgggaag ccgccccgga cctcatcggc ttttccgtgg tcaccaatca atggggttat
     2821 gcccgaaccc tcgcccgctg ggcacgggag gccgtgtcgg tccccctggt catcggggga
     2881 attcatgcca ctgtggcggc gcgggaaatc ctcgagacgg ggctcttcga ctatgtcttt
     2941 cggggagaat gtgaagaggc gtttctggaa ttcgtcgaga aatttcaacg gggagaagac
     3001 ctgcacgatc tctcgaacct gggcctggtg taccggggat ccgtgcgaat caaccccctg
     3061 cgccgcctgc ccgacttgac cgccctccct cccaaggatt acgggattct tgacttccag
     3121 gagatgatcg acgccaaggg cggctgggtg ggcctcatgg ggtcccgggg ctgcccgttt
     3181 tcctgcacct actgcttcaa ccacctgatg gtgcaacagt accgcaaaga cctgggctgc
     3241 gggtttcgtg acctgggcta catacggcac ttccgcgtgg atcaaatcat cgcggagatc
     3301 caatacctga cggcgaacta ccgaaatatc cgaatgttca tcttcgacga cgatcttttt
     3361 acctttcgga aagggttcgt gaaggaattc tgccgggaat acaaacgggt atccgatatc
     3421 ccgttcgtgg tgaacgctca tgtcggcttc ttcgatgaag accgggcgcg ggcgcttgcc
     3481 gacgccaact gtcgaatcgt gaagttcggc gtcgagagcg ggagcgagag gattcgccgc
     3541 cggatcatga atcgccacac cagcaacgag aagatccttt cctccatccg catcgcccgg
     3601 aatttcgggc tccacgcctc catcttcctc atgatcggat tgcccttcga aacccgggag
     3661 gacgtcatgg aaaccgttcg cctcatggcc acggccaagc ccggccggtt tcgatggtcc
     3721 ttcttctttc cctttcctgg gacgagggct cacgagctca gtctccaagg cggctacatc
     3781 gatctcgaga agatgaacac cctcatgaac ttcacggatt cctcctgctt gaaattcggc
     3841 gaggaacacg acctgttcct ggaaaaggtc ggccgggtct ttccctggtt tgtcaacgcc
     3901 cattcggatc tgcccgttcg ggactattac cggcgacggg tggaagacct tctcgccctg
     3961 tccgcctcgg aatgggagaa ggcatccccg ggactgatcg aggaagatgg cgtcctgtcg
     4021 gaggccttcg cccaaaaggg cctttcccac tacgccgtga agtacaatcg gttcatggga
     4081 gtgatctccg attactttct ccaagagggg gcttgaggac tcgaaaccga ttcgggacat
     4141 tcccggatcg gggattcccc ccaccctccg cgcatccttc tccttatctt tacaacgatt
     4201 ggaaaagtca ccatatttca ttcacggatt tctggaacag ccggagatcc gggtcctggg
     4261 aatgatggag gtgcttctat ggaaaacaag ctgctgcaat tggcggtgca gattgtgcac
     4321 gctcaggctt ctcatgccaa aatgtcgggt gaggagatcg aggccgtcct catgaaagtc
     4381 tacaactcac tctacaagat agaacaggcg gaagcagaag gtcgtcccct tttgccggag
     4441 gaactcgccg ttgctccaac cccgggagga gagccggcgt tccggtcgga gccccgctcc
     4501 tccatccacg acgacaaagt gtattgcctc gaatgcgggg cggagttcag gcagctgacg
     4561 gccaatcatc tgcgcatgca ccggttgacc ccccgggaat acaagcgcaa gtggggattt
     4621 cccttgaaac agccgttggc cgcgagaatt ctcacgagcc tgagaagtcg ctccgccaag
     4681 aaaaggggac tgcccgtcaa gctcaaggag tacctggaag accggaagaa gagaaagggt
     4741 tacggcccgg atcggggaag cgtgagtcac tcggcatccg gttgagggaa ctgatgtcgg
     4801 tcgtgtttcg gcagggaacc gggcctcgag gatcgttcgt ttcagcccgg cgcggggcgg
     4861 tggagcagcg actaccggcc gtcttttctt gaagctcgct caaagagact ctcgatctcc
     4921 aggcgcgagc gccggatgga ttccatccat tttagcgcct gtcgctggaa gtgcgggggt
     4981 tcctgccgga agagtttgac ctggggttcg cacaggaacc agacgcagac gtagggccgc
     5041 cgggcccggt ccagatcgca ccctcgttct cccaggtacc gacagggctc gccatgccgg
     5101 gcccgggttt gacccggggg cagatccaac ccggaagaag ccaggaaaag caggtccgcc
     5161 cgattgtaaa agatgttctt tccatggcag catcggtccg ggcacgaacc gcaggtgagc
     5221 tcgcagtagc ggctcatcat cgcatccagg cagttcagcc cttgccggat gccgaggacc
     5281 cgggcctgaa cctgccggga atccaggcgc gccaatcctc ttctcaactc caaaacaacg
     5341 gagcgccatt cctcatcctc catgggacgg cggtattggg aggactcctc ggttcgagcc
     5401 gtcaagagac cctcgcgtgg ggattcgggg gcggacatcg tcgtctattc ccctttccat
     5461 cccctgaggg ggcagggttc acaacggggt ttgcgtcggc aaaacaggtg tcccgtgcga
     5521 accaggaggg cgtggtactc ctggaacagg gccgtatccg gctgcaggtt ggacatgaaa
     5581 aactcttgga gctcccagta cgagatttgt tcctgtaccc agccgtgcct cgaaaaaatg
     5641 cgatgggtgt acgcatccac cacgaaggaa ggttggttgg cggcgtagag gagaatgctg
     5701 tcggcggttt cgggtccgat tccccggacg cccaggagtt cttcccgcaa ttgggccgtc
     5761 tcttgaccca ggaaccggcc gaggtctccc tgccagtgct cttccaccca ccggacgaat
     5821 gccttgagct tctgcgcctt ttggttgtaa tacccggccg agcggatcag ggaggccagt
     5881 tggccgacgg gaacctctcc aagggcgtga aggtccagga gttcgttgtc cttgaggacc
     5941 cggacggcct gttccacgtt tttccaggag gtgttttggg tcaggatggc ccctacgatc
     6001 acttccagag gcgtatcggc gggccaccag tgttgagggc cgaattcgtc gaggagtcga
     6061 tggtagatct ccaggagaag ttgctctttc cctgtcattc gttcaggtcg atttctagga
     6121 gattcccacc ccggcaagaa ccgtggaaca ctgttcgcac tttccttccc ggaggcctcg
     6181 aatccgcatg gtgaaaccaa aacggtcgat gagcaggttg ccgcaccggt gacagtaggt
     6241 gttttccccg tcatctcccg ggacattgcc cacgtatacg tagcgcagcc ccgcctcgat
     6301 cccgatctcc ctcgcccggt gcagggcggc cacgccggtg accgggcgat ccgtcatgtg
     6361 gtacatggga tggaaccggc tgatgtgcca ggggatggag ggatccaggg ccgccaggaa
     6421 ccccgccaac cggcgcaagg attcttcgcc gtcgtttagc cccgggatga gcagagtggt
     6481 gacctcgaac cagatccccg cctctttgag cccctccagg gtcttgagca ccggcttgag
     6541 ccgggccccg cactgtttcc cgtagaagtc gtcgtcaaag gccttcaaat ccacgttggc
     6601 tgcatccaga aagggggcga gggactttag ggactctcga gacaggtacc cgttggtgac
     6661 gaagacattt ttgatccccg cccggtgagc cggttcggca acgtccaggg cgtattccat
     6721 gaagatcgtc ggttccgtgt aggtgtagga aatggaggtg cagcgagtcc gtcgagcttg
     6781 ctctacgatc tcgtggggcg gaatttcggg gccccagatg tttccgtact cgacgggggc
     6841 ctgggagatg tccgcgtttt ggcagaagag acaccgaaag ttgcagccca ccgtggcgat
     6901 ggagtaactt cgacttccgg gatggaagtg atacaggggc tttttttcga tggggtccac
     6961 gttcctggcg atcactctcc cgtacacaag gctccacagg gtcccctcgc ggttttcgcg
     7021 cactccgcac ttgccccgct cgttgggtgg gattcggcac tgctgggaac acaggaagca
     7081 atgcaccagg gactcatcga ctcttctcca ccaccgggcc tccctcatgc cccccctccc
     7141 ttccacagcg gacccccttc gtctccgtac gcggcctggg agcggggccc cgcggaattc
     7201 gaccgcttct tgaaggagga ataccagcag gcggatcgca gccctccgtg gcccacggcg
     7261 gagggcaact cggcaaacaa cggctgttgg acggtctgga ggggataggt caaatccagg
     7321 cgcatgccga tgaaatgacc cagaggcagc cattgcatcc accgggagcg ttccagtcga
     7381 tgggtgtact tcagggcacc caggggcagg ctgaggcacg tcctccatcg aagtctcacg
     7441 ggaccgcgga ggatttcctc catcatgtgt cgaaggtccg gaatgctgaa gaaccgggcg
     7501 tatcgataaa cggtaggccg ccagcaagcc tcgatccacc gttcgagggt gatgagggaa
     7561 taacggttga ggactcccag gagcacggtc ctccgggcga cccgcagggc ttcggtaagg
     7621 gcggttacgg ggtcctctac gaattccagg gtggtgatga gggccaccat atcgaaagca
     7681 ttgtcgtcga aaggaaggtg ttcggcgcag cctttatgga agtgaatgtt ccagggcaat
     7741 tggcttcgag cgatctggag catatacggg gagggatcga tcccggttac ccggtgtccc
     7801 tgctgggcaa accattcgga gaagaccccg gtgccgcacc cgacttcgag gaccctctgg
     7861 ggacacgaag gggcccacag gcgggcgagg agttccttct cgacggcaag ggccgtccgc
     7921 cccggctccg accggaacca gtcgcgatag cgcagggaat cttcataacc gaaggtgtaa
     7981 cccacagaag tccaccatcc caatgaggca ccccgacccc aaaaccgggg gggccgccgg
     8041 gagctgcccc ccgcgtcgcg gtgttcccct cggcaacaag ggtcgacgga acctcccgag
     8101 acgcttaccg gctttcttga acagccactc cgcgcagact ccacccaaga cgtcgcccac
     8161 gagcttgggc gggtctcgat ccctcggggc tagcgccgga gaacggctcc ccgggacccc
     8221 gaggtgacca tctgggcgta acgcgcgaga tagccttcct tcacccgggg ttcgggcggg
     8281 gtccatttct cccgccgctg cttcaggacc gtttcatcca ccaggagttc cagcttcttg
     8341 ttgggaatgt cgatgaggat ctcgtcgcct tcctcgacca gggcaatggg tcccccctcc
     8401 gcagcttcgg gggaaatgtg cccgatggcg gcgcccttgg tccctccgct gaaacgtccg
     8461 tccgtaatca aggccacgtc cttgcccaac cccatgccga tgatggccgc cgtcggcgtg
     8521 agcatttcct gcatgcccgg cccccccttg ggaccttcat accggatgac caccacgtct
     8581 ccgggaacga cggctccgcc cagaatggcc gaagccgcgg cggattcgct ggcgaacacc
     8641 cgagctcgac ccttccgaca gagcatctcc gggtctaccg ccgactgctt gaccacggcc
     8701 ccttcgggtg cgagatttcc gtacagaatg ccgatgcccc cttccttatg gtaaggattg
     8761 tccaggggcc ggatgacttc atggtcgatc tttcggacct tccggagatt tgcccccacg
     8821 gtttccccgg tgaccgtcat cccgtccagg tggatcatat gggcatcggc cagcaccttc
     8881 atgattccct ggactccgcc cgcggcgtcc aggtcttcga ggaaatgggc gccgccggga
     8941 cgcaggctgc acaggtgcgg cgtgcgggcg ctgacggtgt tgaaaagatc caggtccagc
     9001 tcgatcccgg cctcattggc gatggcggga acgtggagaa cggtgttggt ggaacagccc
     9061 agggccatgt ccacggccat ggcattttca aaggcgtttc gcgtggcgat atcccgggga
     9121 cggaggttcc gcgagaccaa ctcgatgatt ttcatcccgg caagtttggc cagccggatt
     9181 cgggccgccg tcaccgcagg aatggtcccg tttcccggca gccccagacc cagggcctcg
     9241 gtcaggcagt tcatggaatt ggccgtgaac atcccggcgc aggacccgca cccggggcat
     9301 gcgttctctt ccaattcctc caattcctcg gcgctcatct gtccggtctt gtaggaaccg
     9361 accccctcga acacggatac caggtccacg gggtttcccc ggaacctccc cgaaagcatc
     9421 ggtcctccgc tcaccatgac ggacgggata ttgagacgca aggcggccat gagcataccc
     9481 ggaatgatct tgtcgcagtt gggaacgagg accaagccgt cgaagggatg agccatggcc
     9541 atgacttcca cggaatccgc aatgagttcc ctgctggcca gagcataccg cataccgtcg
     9601 tggttcatgg cgatgccgtc acagactcca atggtgccga actcgacggg agtgcccccc
     9661 gcgtggcgaa ccccggcctt gaccgcctcg gtaatcttgt ccaggtggat gtgacccggg
     9721 atgatttcgt ttacggaatt ggcaatcccg atgatgggac ggtccaattc ggcctgggta
     9781 tatcccatgg ctcggaacaa cgagcggtgt ggagccttct cgattccctt cttcatgatg
     9841 tcactgcgca tatttcttct cctaaatggt ggttctgccg ggtatgcaac cccgcttggg
     9901 cgttacggtc ctttgcgttc ggtatccaat ttcaatagca ctgatgcagg caaaaggaaa
     9961 ggtctctcat gaagccccgg cgaggaaaaa attggactat caaactcgta gattatataa
    10021 ttctttgaat atagacgacc cggcaacggt ccgccctaca ggaggttggt ccatggatga
    10081 tgaaaaccgc aaggctcttt accccatagg gatcgttgcg gaattgctca gcatccaccc
    10141 ccgcacgctg agaatctatg aacaggaggg tttgatccgc cccgctcgaa ggggcggcaa
    10201 gcgcttctac tcgaataacg atcttcagtg gctcaagtgc cttcgcaagc tcctgagcga
    10261 cgagggactc aacatcgccg ggatcaagaa actgctcacc ctggccccct gttggaagat
    10321 ccggggctgt cccgaggaga tcagaaagca gtgccccgcc atcctgaatt tccccgtgcc
    10381 ctgctgggac ctggtccccc gggaatgcaa ggaaaacagc cagtcctgtt ccgattgcga
    10441 ggtctacctg aacaagatga atcacgtcat gatgcagcgg tacaaggaag aaccttcacc
    10501 cgagaaatga ggggtcgcct cgaccagctc ctatgccgac catctttgga cccgtccctt
    10561 cccgccgcct gggaaggtcc ctgggcatcg acgttgttcc ccacaaaaca tgcacctaca
    10621 actgcgtgta ctgtgaagcg gggcccacca cgtgtctgac cctggatcga caagccttcc
    10681 tggaccccga tcgggtcttg ggggaactgg cggactattt ccgaaaccat cccggcagcg
    10741 tggacgttct caccttcgcc ggcgccgggg agcccaccct ttatcttccc ttgggtgacc
    10801 tgatccggga aatcaagaag cgctatcctt cctatcccct catggtcctc accaacggct
    10861 ccctcctctg ggacccttcc gtgcgccggg acctgatgga agccgaccgg gtcgttccgt
    10921 ccctggacgc aaccatcgag gaggtttttc gccgagtgaa ccgtcctcat cctcgcctga
    10981 agctatcccg gatcctcgag gggctgttcg cttttcgcca ggagtatccc cgtcctttgc
    11041 atgtggaggt tgcgctggtt cgcggagtga acgaaagtcg ggccgccttg gagaatcttc
    11101 gggacatctt ggacgcactg gatcccccga gggtcgagct gaacacggtg gtgcggcctc
    11161 ccgccgtccc gggggtgatg gggctcgagc cgcacgagat ggagaaagcc cgccgaatcc
    11221 tgggccgggg tcgaacggag atcatcggga gcttcctgca cccgccggtg gtcgggtcgg
    11281 acgcaaacct ggaggagcgg atcctggcca tgatccgcag acgaccgtgc gacccccagg
    11341 agatggcacg atccctggcc gtggaacccc acgaactgga atccgcatcg aggagactcc
    11401 ggaaaaaggg cgtggtttcg aagcagcgat tcggggaccg ggagtatctg gttcccagtg
    11461 aaaagtaagg agaaaccgtc tcccggatcc ccggttgaat cctacggaac ctatttcagc
    11521 cgggccgtgg agggcggcgt ctctccggcg gcttggggta tcgttcgcac aatcaaggtg
    11581 ttggaaaaca gctcccgggc caactgctgc acctcttcgg ccgtcacggc ctgaaccctc
    11641 tgcatgtagc gatcttcatg gtcccatccc aatcccagta cttcgttgag ggccgccgtc
    11701 cgggcctgtg catcgaggct ctccatttgc agggcctggc tggtgtgaat cgtgtccttg
    11761 gcactctgca gatcttccgg gctgatcggt tctcgggcca ttttcctgag gttctccagg
    11821 acgatttcct gcaccttctc gaggtttgaa ggggtcgttt gggtaaggac cccgaagtaa
    11881 cccgcctttc tcccgtaaaa gggaaagccg gagaccacgt acaccaggtc ttctttgccc
    11941 ctcagggcat caaagagccg tccgcaggga gagcctctcc cggacaacac ggcatccaga
    12001 acatccagga tcggtcgatc ctcgctgtcg acggccagtc cgttggtgcc gataaaaaga
    12061 gccgccgacg tcttctcgtt cttcttttcc accagtcgat cggcggtcag aggccgggtc
    12121 tcgtcgggcc acgggggcag agttgcctga acgcccctcc acccttggaa cttctcccga
    12181 acggtgcggg aggtcttttc cacgtcgaga tctccataga cggccagcac cgagtgcctc
    12241 ggattcacca tcttccggta gaaggacagg atttctctcc gggagaagga tcccacggat
    12301 tcctccgtcc ccagccgatc ctgtccgtag gacgattgtt ggaagtagtt cctcttgaac
    12361 aggcgcagaa cttcggactg ccagttctca tcgagacgac ggagggccat gagggtttcc
    12421 gttcttttct tttcaatttc actctccggg aaccgggcat tgcgcaccag gtcggccaca
    12481 atatccaggg caacatccag gtcctccttg aggaccttca cggcaatatg ataggagttg
    12541 ttgtcggact tgctctcgat ggtgcccccc acgtcttcga tggcccgcag gatgtccatc
    12601 ttgttccgct ttccggtccc cgcggtgagc agcgacgccg tgaaggccga aatgccggga
    12661 cgttgggaac cttccaagga gagtcctccc agtccgtaga gctggatggt gacccgggga
    12721 agagacgcgt cccgtttgag gagggttcga agaccgttgg gcaactcggc aaaccgaacg
    12781 ggcgggtcgt ctgcggccgt tgcgggagcg accggggccg attgcgcggc tttctccccg
    12841 ccgggggggt gagtcgccgc aacgttcata cgccccctgt ccaagtatcg ccgggccacc
    12901 ctgcggacct cctccgccgt cacccggcgg atgccgtcga catacgtttc ctcgaagtag
    12961 gggtccccgg tttccaggta agagacggcc agggaggacc cccgggcgtg cgaggactcc
    13021 ttgccgcaga tgtgttgggc gatgatgctc tttttggcct tctccagttc cttgggcttc
    13081 accggttctc tcttgaaacg gtccatctcg tcgtggatga tcgagatcgc ctggggccag
    13141 ttctggggag gcaaggtcac attgaccatg aattggccgt ggacgtagga tggggtccaa
    13201 ttggtcgcac tgacgctcaa gacctggttt tcccggtctt tgagcctgca cacgaggttg
    13261 cagctctccc cctggcccaa caggagggcc aggacgtcca gggcatagag gtccgggtgg
    13321 tgcaggtcca cggaggggaa cccgaggatc gcctgggtaa gccgcacaat ggggagttcc
    13381 ctctcctgcc ggcggggact cacctggggg ggctccgtcg tcccgggcaa cgccgtctcg
    13441 acctttcgcc ggaaatcctt ggtcttggtg taaacaaact cgatcacctt ccacggatcc
    13501 accctgccgg acaccacgac caccatgttc tcgggatggt atcggcggga atagtagtcc
    13561 tcgagggcgt cccgatccag cttcaggaac acttcctcgt agccgatgat ggggtcccgc
    13621 acggggctgt tgcgataggc cgtgttcatg taaagcttcc acagttcgct cccgggattg
    13681 ttctctccca tcttgatctc ttgctggatg acggactttt ccctggccac ttcctgcggg
    13741 tccaggatgc tttcggtaac gtaggagatg agcaggtcca gggagtcctt ccaatgcttg
    13801 gaagagctat tgatgtaata gccggtgcgg tcgtaggagg tgaaggcgtt gctctctccg
    13861 ccaatggcct gcagtcgttc cttggcctgg gcttccgtga aggatcgggt cgaccctccc
    13921 gaaagcacgt gctcaagata gtgggaaatc cccgatcccc ggtacttccc ttcgtagatg
    13981 agccccgccc tcacaaggac ctgggcggac accacatcgt tttggagctt cggatgaacc
    14041 agcacggtca agccgttctt gaggaccaca aagacgtcac cgggttggga cgataagacc
    14101 tgttgtacaa aaggccggag ggtgtccggt atggagggga tggagaggcg atccccggag
    14161 gagaaaacgg gaccgacaag cccgaaaacc aagacaagaa gcgccccacc aacgacggtt
    14221 ccttttccca tggatttttc ctttctgcag gcactggctc gaacctgact cacaaccgct
    14281 gcaagacgtc ctacaaggag ggcaaaaccc ctcctgtttc aggataggta ctcccttaca
    14341 gaacataaaa tccccggagg gacaaggcgg ccaaggagcc gtgaaccccg gtcctccggc
    14401 cgttcccttg ggatttgcat tccctgtgaa tcggggggcc gaagagctac aggatggatt
    14461 cccggatgag acgcccgacc aactggtgac ggcggaccac accagagggg gtttgaactc
    14521 ggaggggagg cctcagcatc tcgatcagct tccaggattc cccgtcatgt ccccagtgga
    14581 ccagaatgtc gtcgatctgc gccccccgca ggagaccgcg ggtttgacga atcaccgaat
    14641 cgaggttctt ctctcccgtg gaaacgggct cccaggttga agtgccgcat cgagaggcgg
    14701 tcaggacctg tgcgccgatg cacccatgcc gcaggaagac cagtctgacc tgtcgccgcc
    14761 atgagaccag gggtcgaacc agcaccgggt tgtaccggtc ccgcagccct cgcaggtctt
    14821 cgccgcagtt gaccacgtgg accgtatcag gccttgcgaa aggtcccgtg agcagaaacg
    14881 gtgtcgggaa gtcccggaga atggcctcct tctgtcgagg gccatagtag gctcgacctc
    14941 ccagatgggg aatccccacc cattggaata gggcattttg aaggagacgg gagcgctggt
    15001 agcggtaaga gtaataactg gggaagcagg gcagacccgc cgcgtgaaaa accgagacaa
    15061 aacgaggggt cgggaaaaaa atccgggagg ccttacggag agtctgtctt tcttcggccg
    15121 agtagtcctc gaagccgggt cgaactccca gggtgagtac ctccgggcag tcggcaagga
    15181 acggcgccaa ggcaactcgg gaatgttcca ttctctccct ctcgaacctc ccgttcatcc
    15241 gtccgagtca aaggaaaccg ggacacggga ggctcagagg gggccggagt tcaggagcac
    15301 gtcggggtgc actccgtaat tgtagctcct catacggaag taattctcca aaaggctgat
    15361 tcgatggtcc acgtagtcat agatcttgag cttctgatcg ttgccgttga cgtacaggtt
    15421 acgtaaggcg ttggccagat gctttcggaa gtagatgggc acggccagga acagcacccg
    15481 tgccttgagg ttcgcggaac actgggcgag ggtgttggga gtgacgagca cggctacggg
    15541 acccgtcggc cattccacgt tctcaggaca cgcgtcggga agagggggtt cctcgacatc
    15601 ctcctccccc gcaagcgatt ccaccttgag gggcattacg ggaatgcctc gtttccccag
    15661 ctccgcttcc agggctccct gtcccaccgt ttccgtagtc agaaccagga gaggttgcgg
    15721 aacttcccgg agctccgatt cgatgtcgtc ggcgatgagg cgcgttctcc cttcatcccg
    15781 ggccagggct tccaacatcg gctgataatc cgaccgggag tgataggggt attcgaaggg
    15841 ggtaggccgt gccaccacgt gggcatggat gatccctcgg ccctcccggg catccttctc
    15901 attgatcgaa tagacgacgt ctcccaggtg gtagtacacc agccgcgaca gccgatcctt
    15961 gcgctgggcc gtattggaaa gacccagcat gtagcggcag tcgaagttgg ggatgaggta
    16021 cgtcaggacc ttggaaggac atcgctgaca ctcgtccacg atgaggaacc cgattcggtc
    16081 ccagagcttc ctccaatgtc gcatcacctc cccggtgtgg gccacggtga tggtgttgcc
    16141 gatctggtgc acccctcccg agaacactcc cacctcggaa cctggaatct gtaggaaatt
    16201 tgagatctta gtgagccacc cgtccagaag gtccagcttc gggatgagga tcaacgtggg
    16261 ctggcgcctc tgggcaatgg catagagggc aatcaccgtc tttccactct tgtgcccgcc
    16321 gatcagagtg gcaaactccc gctccaggat cgcctccgcc gcttcctgct gataggactt
    16381 cagctccccg tggaattcca gggacaaggg ttcgaaggaa cgtctcttgt cgacgattcg
    16441 gtaaggctgc tgatacttct tgcaaagctc ctgaagctgg tccaggaaac ctctgggcaa
    16501 aaggtaatgg cggccctttt gtcggatgca gctgatttgg gcaggaatat tcccgatcca
    16561 ttctccccgg ttgtggcgtg cctcgtagtc gggattggtg aagaccaggc gttcatgaat
    16621 ctttcgttcc aagagaggag acaagccgtc cgaaggaatc ttgatccatt ccgcaagcac
    16681 gatgttgagc gtctttacga acattcacca cgacctttct agcattggct tttcccaaag
    16741 attggattca ttccgctact ccggatctcc aatgacggag aagattcctc tcttgtcgcg
    16801 ttcaggacca cccacggaaa cggggggacg gttggttgtt cgattggctt ccatttcgac
    16861 ggtctgccca cttcgggttg agttaaggag tttgcaattt tcatacccga accccgcaac
    16921 cggaaaccaa cccctgtccg ccaccgagag aaccggtccc cacgggccaa gagagttccc
    16981 gaggaccgcc cggcggctcc ccttgccctc acggagccaa aagcgacccg ttcgcaggaa
    17041 ccgtttgaca gctccttctg attccaatag gttaaacgcc gaggccattt tgacccacac
    17101 atcggaggaa cctggggtca ggttcgtcca ctccccggga acccgcttgg agcaaaggac
    17161 tttatcgtgg aagaccagtc gtcggatcat cttctgatcc ttaagcaact cgccctggaa
    17221 taccagcaga agtacggaga actggagcaa ttcgtccgga acaccgaacc ccaacggctg
    17281 ctcattcaac tgcgcaacca gggggagctc gccacttctc gattcaggtc cttcgaggtc
    17341 gcactcctca tggctctgtc tcaacgggcg ggggaacagg acggcgacgc aaccctccgc
    17401 ctggccacgg acttgtgtcg gtgtttcgac gaaatgagaa tcctgtttca
//
LOCUS       DAOOVC010000079        17440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2235_length_17440_cov_7.572793, whole genome shotgun sequence.
ACCESSION   DAOOVC010000079 DAOOVC010000000
VERSION     DAOOVC010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17440
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2235_length_17440_cov_7.572793"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLM79_10905"
     CDS             <1..482
                     /locus_tag="PLM79_10905"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOV86863.1"
                     /translation="SPSASKVPFIVIRISLGIVFFAASADKILHPAPFARIIHNYQIL
                     PSSLVNLTAIILPWTELLLGLFLLAGWWLYGTVLLSGALFTVFLGALAFNAARGLDIH
                     CGCFSTSLEAGGGTVWTMVRDLGFWVMALYLGCRLFLRGRPSERGKQGEDPRCPPLT"
     gene            561..1367
                     /locus_tag="PLM79_10910"
     CDS             561..1367
                     /locus_tag="PLM79_10910"
                     /inference="COORDINATES: protein motif:HMM:NF025729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HOV86864.1"
                     /translation="MRKQRFRGFMTLLGMFVLAWALADPALADIFMKQKQHTDGFTMM
                     GQTQPPKDTVQTLWMSADKLRSDDDKHSVLILLDKQTVYLIDHARKTYQEVPMDPGKM
                     AAEAMGDDEDMTPEEKQQLMNMARGMMKMEVTVVETGEKKKIGNWNCKKYLQTVNTMM
                     GPMTSEIWATEDLKVDYSLYARFSAAMLAMQPGMGKNFNNLAEEMKKIRGVPVLTTTT
                     TKVMNSTMKSSQELLEFKDAKAPQGTFDLPRGYTKQAMGGGAGGPGMRRP"
     gene            1501..2115
                     /locus_tag="PLM79_10915"
     CDS             1501..2115
                     /locus_tag="PLM79_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86865.1"
                     /translation="MPFIRFPGLCVCMMFALGFCLVIPGGSVRADEVIDSIQQAMDSY
                     KKGDFTEAENNLNYASQLIRQKKSSDLEAFLPAPLTGWKAGEVKKQALGQAMFGGMVS
                     VERDYRKGSSRVTVKIVTDSPLVQSMMMMLTNPLLATSDGGTMKKIGGRKAVVKFKDG
                     NQRGEINLIVANRILVTVEGSEVTEKDLADYASAIDYKKLEALQ"
     gene            2749..3318
                     /locus_tag="PLM79_10920"
     CDS             2749..3318
                     /locus_tag="PLM79_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOV86866.1"
                     /translation="MSRENIGARIRGFREQRGMSLEELADRTGLDNRFVEAVEQENVY
                     PSLGPLLKIARTLGIRLGTFLDDQLTDDPLIVRLEDRKEELSMLTRKGRPVALRFYSL
                     GRGKSDRHMEPFFIEVLPESATEKKLSSHEGEEFIVVLSGEIEVIYGQETHVLKKGDS
                     IYYNSVVPHYLSCKGDHKAEIYAVLYFPE"
     gene            3337..5004
                     /locus_tag="PLM79_10925"
     CDS             3337..5004
                     /locus_tag="PLM79_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV86867.1"
                     /translation="MDQPALFPLTLGQILDRAIAAYPDNDAVVYTDRNFRLTYREFGN
                     LVDRLAKGLMALGVQKGEKIAIWATNVPYWVVLQFASAKIGAILVTVNTLYKITELAY
                     LLGQSESENIFIIDGYRDTDYLQTLYELVPELKTHQRGYLKSPNFPHLKRVFFLGHEK
                     HRGMYSIAEVLALSSMVGDEEYLARQSLLDTHDVVNMQYTSGTTGFPKGVMLTHYNIG
                     NNGYWIGENQALGPRDRVCLPVPLFHCFGCVLVVLAAVSHCSTLVILETFDPVQVMAS
                     VEMEKCTAMNGVPTMFIAVLDHKLFNKFDYSSLRTGIMAGSPCPAEVMRRVMDKMNMK
                     EITICYGLTEASPVVTQTRVHDDLRKRVETVGRPMPEIEVRLVDPETNVPVGIGVQGE
                     LCCRGYNVMKGYYNMPEATAQAIDAEGWLHSGDLAVMDEDGYISITGRYKDMIIRGGE
                     NIYPREIEEFLYKMPGILDVQVVGVPSRVYGEEVGAFVVLREGFDLTPEDIRDFCRGK
                     IARYKIPKYVAFVKSYPMTASGKIQKFKLREMAAELFGETGQKGGGS"
     gene            5001..5759
                     /locus_tag="PLM79_10930"
     CDS             5001..5759
                     /locus_tag="PLM79_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV86868.1"
                     /translation="MKCPKCGADQRDESPECSRCGIVFEKYRAYLESRERDGHSETAA
                     LGDPDAQETGGLWNSLKEYVLFVEPRVNPFHLVGRILVYLLLFFWGWKFILTPMESNY
                     VGSSFLHAVNLPFHEAGHVIFSFLGEFMGVLGGFLGQLIMPLVCFGALLFGTRDPFGA
                     SAALWWTAESLMDSAPYINDARAQVLILLGGVTGREAPGFHDWNNLLGRLGLLPHAHA
                     IARTVYSLGIALMLVTFLWGAVLLYRQYKNVESL"
     gene            complement(5833..6789)
                     /locus_tag="PLM79_10935"
     CDS             complement(5833..6789)
                     /locus_tag="PLM79_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86869.1"
                     /translation="MEKRILLTVDGSEKGFKAVSIVGDLLKDQTGYRLVLYHCVQQLA
                     RFYPGELCEVEQSCRLPLSDQEKVGCAVLDESKRLLVGAGFPADRIDTKLKMDSTDPA
                     LDILREAEGEKIRTIALGRRGRGQLETLLLGGVTSKVAQYARYRTVWIVDTPVFQTRK
                     VLVALEGASDARTLTPYAAEYLAPISDLHFTFLHLMPPVPPTFWDDGHILDPSERKDR
                     QARIEKWRSDWIEQAEEFLEEARGQIAGCGVPRERIRTHVLPTKQGIARDLLTEIAEH
                     QYQVVVMGKRSFHERKPFLLGSHANKVMQNVQGAVLCLVDAQ"
     gene            complement(7005..8918)
                     /locus_tag="PLM79_10940"
     CDS             complement(7005..8918)
                     /locus_tag="PLM79_10940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOV86870.1"
                     /translation="MQRYFGPEGILAQKLEHFEYRPSQLEMAQTVYHCLKTEVPLMVE
                     AGTGTGKTWAYLVPAILTGKKIVVSTGTKTLQDQILDQDIPLLKKLLFPRLQAVCLKG
                     RGNYLCRRRFSEFAYQPAFHSREEAKLFRRFQQWAGRTKSGDRAEIPWLPDQFQAWSE
                     VCSGTEHCLASQCEDQSRCFVHRLRMEAAQAQLVVVNHHLFFADLALRKKALTEVLPD
                     YDGVVFDEAHQLEDIVGLYFGVQFSSRGLRELALDLARECRKVLPKGEKVRDLLKTAQ
                     HLEALTRDFQHTMQGFAGSGGRFLLDSPRVGEGFDRVCGKVVRALERMGERLLSFREE
                     NPVLDSLDRRGAEMARGITQLQARKDASLIYWFEVNHGSVVCHGTPVEVSSILEECLF
                     ARRSAVVLTSATLTVGSSFQYLRESLGLPSETRELSAPSPFAYEDQAVLYIPPDFPSP
                     TDPTFCRCLAEQALLIFRKTRGRGLFLFTSYRNLHQVHELLKDRLPYPILVQGQKPKR
                     VLLAEFKERIDSVLMATSSFWQGIDVPGESLTCLLIDKVPFEVPDDPIGSSRMKHLES
                     RGKNAFYQYQVPRAVIQLKQGLGRLIRSSRDRGVMAVFDVRLLQKSYGALFLQSFPPC
                     RRVRGIEDMDAFL"
     gene            complement(8930..11299)
                     /locus_tag="PLM79_10945"
     CDS             complement(8930..11299)
                     /locus_tag="PLM79_10945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOV86871.1"
                     /translation="MNHSDSSRRVPKKSSFRWLVFTLLGLGFCLVLGVTAILGGFYLQ
                     LTRSLPNITSLKNYHPPLVSSVYASDGTLIGEFFAERRYLVPLEELPKALVQAFLAVE
                     DVRFYEHGGVDLMGIIRAMFKNIQAGEIVQGGSTITQQVVKSLLLTPERTWIRKLKEA
                     ILAYRIDHYLTKDEILYLYLNQIYFGSGAYGIEAAARTYFDKHAKDLKLNEIALLAGL
                     PKAPSRYSPFVKYSVARERQQYVLQRMTEVGFITQDEARQAFNAPLDLVKPKHWTLKE
                     LDHFTEEVRRQVEARYGREILYKEGLRIHTSLDPRAQQIAKKALDQGLRELDKRHRRY
                     RGLHENIPKDDWPQYLTQLAARNGELGPGKVVGALVLSYDPKAKTCLLHLGSSQGKLP
                     ATGWQWAGLSVTRAEKMFRPGDVIRVRLDKQQDGRTWLAALEQDPGMEGALLAMEVET
                     GRVVSMVGGRDFEKSQFNRCTQSLRQPGSSFKPIIYAAALDKGYTEASVLIDSPFVRP
                     DHSLRGVWAPSNYDRQFWGPILLRKALIHSRNVVTVKLLDDIGVHYAIDYARKLGITS
                     PLTPTLALALGSSGVTLMEMLTAYSAFANLGERVEPTLIDRIYDRDGRLLEERQVRRE
                     SVISPQTAYLMNHLLQGVIEEGTGQRAKKLGRPAAGKTGTTNELKDAWFIGYTPNILA
                     GVWIGYDDHTIPLGKGETGGRAACPIWVYFMEEYLKNEPILAFPIPPGIVFARVGQSA
                     GTEENPEGSRGVLAAFAGAPPTSTKVARDSGESPPPSSSAESFFKSDLF"
     gene            11582..12610
                     /locus_tag="PLM79_10950"
     CDS             11582..12610
                     /locus_tag="PLM79_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOV86872.1"
                     /translation="MKALHFITHGGPEVLRYGDVPEPVPGPRQVRVRVRACAVNHLDV
                     WVRRGWPGLDLSMPHWGGSDIAGVVDSVGSEVTQWRVGDRVVANPGWCPVEDEFTLRG
                     EHSMSPQYRILGEQVPGGFAEYALVPAGNLAAMPDSLDFPEAAAPLVVTITAWRMLIH
                     RAGLRAGESVLIVGAGGGVNTMAVQIARLAGATVYVVAGGPEKARKAEELGADYVIDR
                     SLRDWDKEMLKLTRRRGVDVVVDNVGAATLPTSMKVAARGGRIVIVGNTSGPLAELDL
                     RYIFSKQIQLIGSTLGNHQDFLEVLALLWKGKLRPVIDRVMPLSRGREALQRIEHGDP
                     FGKIVLVP"
     gene            complement(12646..14046)
                     /locus_tag="PLM79_10955"
     CDS             complement(12646..14046)
                     /locus_tag="PLM79_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86873.1"
                     /translation="MSKLPASQVKILVVDDERSMREFLEIMLRKDGYWVQTAANGKEA
                     LDLIRKNFFDLVITDIRMEPVGGLEVLKRCKRVSPQTVVIIISAYASTETAVAAMNEG
                     AYDYLPKPFNIEEMRAVIRNALETRGGEEDRPARPGDGPLHFGCLIGESPAMRKVYEL
                     IQRVAATSSNVLVTGESGTGKELVAKAIHRQSPRRDQPFVVVNCGGVPESLIESELFG
                     YRKGAFTGATTSRKGLVEAAQGGTLFLDEIGELSPVLQVKLLRLVQDKTIKMIGDTVD
                     ISVDVRIISATNRDLEKMVIEGTFREDLFYRLNVLPIRIPPLRDRGEDVPILAQHFLN
                     KFCRQFGKDIRKISSYAMDILNHYDFPGNVRELEHIIERGVALESSSIVLPDSLTLAT
                     YKKIRTDSLLPGRPTIPPEGMNLDEYLAEIEKKILQQALQQTQGVKFKAAELLSISFR
                     SFRYRLIKYGLATEEE"
     gene            complement(14043..15359)
                     /locus_tag="PLM79_10960"
     CDS             complement(14043..15359)
                     /locus_tag="PLM79_10960"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOV86874.1"
                     /translation="MEREKGPGSYLFRGGRVIDPTHEIDLCADVLILNGQLAEIRPRI
                     ELGADKGAEVREIDVSGKWIVPGLIDMHVHLREPGQEYKETIASGTSAAVAGGYTSVA
                     CMPNTSPVNDCAAVTQFILQRAREEGSCRVFPVGSISKGLLGENLSEFGELKDAGAVA
                     VSDDGRPVMNSLMMRRALEYAGAFDLLVISHAEDLQLSEGGLMNEGPVSTLLGLRGIP
                     GVAEEAMVARDLLLAEWTRSRIHIAHVSSAGSVRLIREAKRRGVMVTAETAPHYFTLT
                     DEAILSFDPVFKVNPPLRRTEDVQAVKTALADGTIDTIATDHAPHSVIEKDIEFEFAA
                     NGIIGLESALPLILELVREGTLSPSDAVAKVSTNPARILGLPLGTLARGNPVDMTILD
                     PDEIYRLTPETLRSKGCNCPFLGKDLQGRAVMTFVRGIVAYSRLDS"
     gene            complement(15451..16368)
                     /locus_tag="PLM79_10965"
     CDS             complement(15451..16368)
                     /locus_tag="PLM79_10965"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOV86875.1"
                     /translation="MSLKRKDVLGIRELEADEIEHVLNLAASLKEINLRPIKKVPTLR
                     GKTIIHLFYEPSTRTRTSFDIAAKRLSADTYSIAASTSSLVKGETFLDTLKNLEAMKP
                     DVFIIRHPASGTPHRLAEHTAASVINAGDGMHEHPSQALLDMLTIREHKGKLEGLTVV
                     IVGDIAHSRVARSNIWGLRKMGAQVIVCAPPTLMPVDMDSMGVQVCRRIEEAIPEADV
                     LMVLRLQKERMQQMLLPSLREYSIYYGINSSRMEKAKKEVLIMHPGPVNRGVEISPEV
                     ADGSRSVILDQVTNGVAVRMALLYLLGQR"
     gene            16613..17083
                     /gene="greA"
                     /locus_tag="PLM79_10970"
     CDS             16613..17083
                     /gene="greA"
                     /locus_tag="PLM79_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOV86876.1"
                     /translation="MQRVPITKDGFERLKTELQRLQKEERPVIIRAIEEARGHGDISE
                     NAEYEAAKEKQGLIERRILDLTQKLSECQIIEPSDEGNGKVVFGSTVVMEDLETHEVT
                     TYRLLGPYEADVGSGTISVTSPLGKALIGKEEGEEVRVQTPKGIRNFEILEVLY"
     gene            17248..>17440
                     /locus_tag="PLM79_10975"
     CDS             17248..>17440
                     /locus_tag="PLM79_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86877.1"
                     /translation="MRCKSMVAVAAFFPAVLWLAPMAMGAPFCRQKDFEGVWNVYAMG
                     SGEWGPWWQACKMVVARDGM"
BASE COUNT         3570 a         5079 c         5191 g         3600 t
ORIGIN      
        1 ggtctccatc ggcttcaaag gttcctttta tcgtcatccg aatctccctg ggaatcgttt
       61 tctttgcggc gagtgcggac aagatccttc atccggcccc ttttgcccgg atcatccaca
      121 actaccagat cctgccctcg agcctggtca acctgaccgc catcattttg ccctggacgg
      181 aattgctcct gggcctcttc ctccttgccg gatggtggct ctacggaacg gtgttgctca
      241 gcggcgctct tttcaccgtt ttcctggggg cccttgcgtt caatgcggcc cgggggctgg
      301 acatccattg cgggtgcttt tccacctccc tcgaggccgg agggggaacc gtctggacca
      361 tggttcggga cctgggattc tgggtcatgg cactgtattt gggctgccgt ctctttttga
      421 gagggcggcc ctccgaaaga gggaagcaag gggaggaccc gaggtgccct ccactcacct
      481 agacgaacct tttgcccgtc aaccaccccc gttgttgcgg agaatccagt caaccaccgg
      541 accggtaacg aggagatctt atgagaaaac agcgatttcg aggtttcatg acccttttgg
      601 gaatgttcgt tcttgcgtgg gccctggccg atcctgccct ggccgacata ttcatgaaac
      661 agaagcagca cacggacggc ttcaccatga tgggccaaac ccagcccccc aaagacaccg
      721 tccagaccct ctggatgtcg gcagacaagc tgcgcagcga cgacgacaag cactccgtcc
      781 ttatcctcct ggataaacaa acggtctacc tgatcgacca cgcccggaag acctaccagg
      841 aagttcccat ggatcccggc aagatggccg ccgaagccat gggggacgat gaagacatga
      901 ccccggagga aaaacagcag ctcatgaata tggcccgggg aatgatgaag atggaggtga
      961 cggtcgtcga aacgggggaa aagaagaaga tcggcaattg gaactgcaag aaatacctcc
     1021 agacggtcaa caccatgatg ggtcccatga cctcggaaat ctgggccacc gaagacctca
     1081 aggtggacta cagcctctat gcccgtttca gcgcggccat gttggccatg cagccgggca
     1141 tggggaaaaa cttcaacaac ctggccgagg aaatgaaaaa gatccgcgga gtcccggtct
     1201 tgaccaccac cacgacgaag gtgatgaaca gcaccatgaa atcatcccag gagctcctgg
     1261 agttcaagga tgcaaaggcg ccccaaggaa ccttcgatct gccccgaggc tacaccaagc
     1321 aggccatggg gggaggagcc ggcggacccg ggatgcggcg cccgtaaaac cttttcatcc
     1381 gtcatgcttt ccctcgcttt ccccgagggg ttttccgtgc ggctttccca ccgtcccatt
     1441 cacggggacg gtttcatggg cacggcagga aaccggtttt ccatcgttaa ggagaccgtc
     1501 atgcctttca ttcgattccc aggtttgtgc gtatgcatga tgtttgccct gggcttctgc
     1561 ctggtcatcc cgggcgggag cgtccgggcg gacgaagtga tcgattccat ccaacaggcc
     1621 atggacagct acaagaaggg ggatttcacc gaggccgaaa acaatctcaa ctacgcatca
     1681 cagctcatcc ggcagaagaa aagcagtgat ctcgaggcgt tccttcctgc tcccctgacg
     1741 ggttggaagg ccggcgaggt gaagaagcag gccctaggcc aggcaatgtt tggtgggatg
     1801 gtttcggtgg aacgggatta ccggaaaggg tcctcgagag tcaccgtgaa gatcgtgacc
     1861 gattcccccc tggtccaaag catgatgatg atgctcacca accctctcct cgccacctcc
     1921 gatggaggaa ccatgaagaa aatcggcggt cggaaggcgg tggtgaaatt caaggacggc
     1981 aatcaacggg gcgagatcaa cctcattgtg gccaaccgta tattggttac ggtggaagga
     2041 agcgaagtaa cggaaaagga tctcgcggat tatgcgtccg ccatcgacta caagaagttg
     2101 gaggcccttc aataacggag tcgcatcctg gtcttcgaaa gactgccgtg cccccgtagg
     2161 gtaagcccat ggccgggccc cggcaaaatc ttcatgtttt cgaggcggtg gccccatctc
     2221 tccgttgcac cggtgtcgcg gcagagcttt gctccccccc cggaatgcgc tccgcacttc
     2281 cttcgggagg ccttttccct cacctccggt cgccggggtg gacggcgggg gacctgatgc
     2341 tgcggccctc gatccgtctt ggcccctacg gggaagcaat cggttacgga agactctcgg
     2401 cggccggagt ccccctcggg gacgcagcgc ctcattttgc cctttcggaa tctcgccaac
     2461 caagctataa agcccataca ggttgattcc gcagtcgcag gacattggga atccggcctg
     2521 tgatgcgggg cttcgcgccg gagcaggggg agggtccaac atttggagcc caaagaagtc
     2581 cgccggtgtt ccctcgcaga tcgtcgcttc tcgtttcaag ttccgacacc aaccacccat
     2641 tttcatgact cgtcttgccc ccggcgggac ggcgccgtgc tgtgattgaa aacgagcggg
     2701 aattctccgg aaaatgtccc agacaaccca tgatgaggag aggcagcagt gagcagggaa
     2761 aacatcggcg cacgaattcg aggttttcgt gaacagagag ggatgagctt ggaggagttg
     2821 gcggaccgca cgggtctgga taaccgcttt gtcgaagcgg tggaacagga gaacgtatat
     2881 ccgtccctgg ggcctttgct caaaatcgcc aggactctgg gaatccgtct gggaaccttt
     2941 ctggacgatc aactcaccga cgaccccctc atcgttcgcc tggaagaccg gaaagaagag
     3001 ctcagcatgc tcacgaggaa gggaagaccc gtggccttga ggttttattc tttggggcgg
     3061 ggaaaaagcg accggcacat ggagccgttc tttatcgagg tcttgccgga atcggccacc
     3121 gaaaagaagc tttcttctca cgaaggagaa gaattcattg tggtcctttc gggggagatc
     3181 gaggtgattt acgggcagga aacccatgtt ctgaagaagg gggacagtat ctattacaat
     3241 tccgtcgttc ctcactatct gagctgtaaa ggcgatcaca aggcagaaat ctacgcggtg
     3301 ctctactttc ccgaataacg aacacgtgag gttaccatgg accaacccgc tttgtttccc
     3361 ttaaccctgg gtcagatcct cgatcgggcc atcgcagcct acccggacaa cgacgccgtg
     3421 gtttacacgg accgcaattt tcgcctcacc taccgggaat ttggaaatct cgtggaccgt
     3481 cttgccaagg gtctcatggc tttgggagtc caaaaggggg agaagattgc catatgggcc
     3541 accaatgttc cctactgggt ggtgctccag ttcgcctcgg ccaagatcgg ggccattctc
     3601 gttacggtaa acaccctcta taagatcacg gaactggctt atctcttggg tcagtccgaa
     3661 agcgaaaaca tattcatcat cgacgggtac cgggatacgg actatctcca gacgctctac
     3721 gaactggttc ccgagttgaa gacccatcag cgcggttacc tgaaaagccc caactttccg
     3781 cacctcaagc gagtcttctt cctgggacac gaaaagcatc gggggatgta ctccattgcc
     3841 gaggtgttgg ccctgagcag catggtcggc gacgaggagt acctggctcg ccagtcactc
     3901 ctggacaccc acgacgtggt gaacatgcag tatacctccg gcaccaccgg tttccccaag
     3961 ggcgtgatgc ttactcatta caacatcggg aacaacgggt actggatcgg ggagaaccag
     4021 gccctgggtc cccgggatcg agtctgtctt cccgtaccgt tattccactg cttcggctgt
     4081 gttctggtag tcctggcagc ggtctcccac tgttccaccc ttgtgatcct ggagaccttc
     4141 gatcccgtgc aggtcatggc ttcggtggag atggagaagt gcaccgctat gaacggggtc
     4201 cccaccatgt tcatcgccgt tctggatcac aagctgttca acaagtttga ctacagcagc
     4261 ctgcgaaccg gcatcatggc aggatcgcct tgtccggcgg aagtcatgag gagggtcatg
     4321 gacaagatga atatgaagga gatcaccatc tgctacgggc tgacggaagc ttcccccgtc
     4381 gtgacccaaa cgcgggtgca cgacgacctt cggaagcggg tggaaaccgt gggacgtccc
     4441 atgccggaga tcgaggtgag gcttgtggac cccgaaacca atgttcccgt gggcatcggc
     4501 gttcaggggg agctgtgctg tcgcggctac aatgtcatga agggctacta caacatgccc
     4561 gaagccaccg cccaggccat cgacgccgaa ggctggctcc actcggggga tctggccgtc
     4621 atggacgaag acggatacat ttccatcacc ggtcggtaca aggacatgat cattcgaggc
     4681 ggcgagaaca tctatccccg ggaaatcgag gaatttctct acaagatgcc gggaatcctc
     4741 gacgtacagg tggtgggagt ccccagccgg gtctacgggg aggaggtcgg ggccttcgtc
     4801 gttctgcggg aaggatttga tctcactccc gaggacatcc gggatttctg ccggggcaaa
     4861 atcgctcgat acaaaatccc caagtacgtt gccttcgtca agagctaccc catgacagcc
     4921 agcgggaaga tccaaaaatt caagctgcgg gaaatggctg cggaattatt tggggagacc
     4981 ggccaaaagg ggggcgggag ttgaaatgtc ccaagtgcgg ggccgaccag cgggatgaaa
     5041 gtcccgaatg ctcccggtgc gggattgttt ttgaaaaata ccgggcttat ctcgagtccc
     5101 gggaaaggga cggacattcg gaaacggcgg cactcggcga cccggatgcg caagagaccg
     5161 gagggctttg gaacagtctc aaggagtacg ttcttttcgt agagccccgg gtcaatccct
     5221 ttcatctggt gggaaggatc ctggtttatc tgctgctgtt cttctggggc tggaagttca
     5281 tcctgacccc catggaatcc aattacgtgg ggagctcttt cctccacgcc gtcaacctgc
     5341 cttttcacga agcgggccac gtcattttct cttttcttgg ggaattcatg ggagttctgg
     5401 gagggtttct cgggcaactg atcatgcccc tggtatgttt tggggcgctc cttttcggca
     5461 cgagggaccc tttcggcgca tccgcggcct tgtggtggac cgcggaaagc ctcatggact
     5521 cggcccccta catcaacgat gcccgggctc aggtcttgat cctcctgggg ggagtaacgg
     5581 gaagagaggc accggggttt cacgactgga acaaccttct gggaagattg ggcctgctgc
     5641 cccacgccca cgccattgcc cgcacggtct actctctggg aattgccttg atgctggtga
     5701 ccttcctctg gggggcagtc ctcctctacc gccagtacaa aaatgtggaa agcctttgag
     5761 agcgcccgga cgaggtcgtc ggaggactcg gtctttccgt ttttcccctt tcaggacgaa
     5821 ggagaaaggc ctttactggg cgtccacaag acaaaggacg gccccctgaa cgttttgcat
     5881 cactttgttg gcgtgactgc ccaacaggaa cggctttctt tcgtgaaagc tcctttttcc
     5941 catcaccacg acctggtatt ggtgctcggc aatttccgtg agcagatccc gggcgatccc
     6001 ctgtttggtg ggcagcacgt gggttcgaat ccgttcccgg ggaacgccgc acccggcgat
     6061 ctgtccccgg gcttcttcca ggaattcctc cgcctgctcg atccagtcgg acctccattt
     6121 ttcgatccgc gcctggcgat cctttctttc ggagggatcc aggatatggc cgtcgtccca
     6181 gaaagtggga ggaacgggag gcatcaagtg caggaaggtg aaatgcaggt ccgaaatggg
     6241 ggcgagatat tcggctgcgt agggcgtgag ggttcgcgca tcggaggctc cttccagggc
     6301 cacgaggact tttcgcgtct gaaaaaccgg agtgtccaca atccatacgg ttcggtaccg
     6361 ggcgtattga gcgaccttgc tggtaacgcc gcccaggagt agggtttcca actgccccct
     6421 gcctcgcctg cccaaagcga tggtgcggat cttctctcct tccgcttcgc ggaggatatc
     6481 caaggccggg tccgtgctgt ccatcttgag ctttgtgtcg attcgatccg ccgggaaacc
     6541 tgccccaacg agaagcctct tggattcatc caggacggca catcccacct tttcctggtc
     6601 gctcaacgga agcctgcagg actgttccac ctcacagagt tctcccgggt aaaaacgggc
     6661 gagctgttgg acgcagtggt agaggaccag gcggtacccc gtctgatctt tgagcaggtc
     6721 ccccacgatg gagactgcct tgaatccctt ctccgaaccg tctaccgtca gcagaattct
     6781 tttttccatc cttctctctc cctgccccta acccggcatt ttcggcacat cgcggggcac
     6841 ggggatttgg ccgggccttc gggttttcga gtcgggccga gccccgtgcc tcgcccgggt
     6901 tgttctatga gccgtccgcc ttccggagga atgaacaacc ccatggacct cactatctca
     6961 ttttaagaac tatttcacaa ctcaaaacat cccggcggag ggtcctacag aaatgcatcc
     7021 atgtcctcga tcccccggac ccggcgacag ggaggaaagc tttggagaaa aagggctccg
     7081 tagctcttct gaaggagcct cacatcgaaa acggccatga cgccgcgatc ccgggaagac
     7141 cggatgagcc tccccagccc ctgcttcaat tgaatgacgg cacgaggcac ctggtactgg
     7201 taaaaggcgt tcttcccccg ggattccagg tgcttcattc gcgaggaacc gatggggtcg
     7261 tcggggacct cgaaggggac cttgtcgatg agcaggcacg ttagggattc cccgggcacg
     7321 tcgatcccct gccagaaaga gctggtggcc atgagaacgg aatcgatcct ttccttgaat
     7381 tcggcaagaa gcactctttt gggcttctgg ccctgcacca ggatgggata gggaagccgg
     7441 tctttgagaa gctcatggac ctggtggaga ttgcgatagc tggtgaaaag aaaaagtccc
     7501 ctgccccggg ttttgcggaa aatgaggagg gcctgttcgg ccaggcaccg gcaaaaggtc
     7561 ggatccgtag gagaaggaaa atccggggga atgtagagaa cggcctgatc ctcataggcg
     7621 aagggggagg gagcggacag ttcccgggtt tcggaaggca aacccaaaga ctccctcagg
     7681 tactgaaagg agctgcccac cgtgagggtt gccgaggtca gtaccaccgc cgatcggcga
     7741 gcaaacaggc actcttccag gatggaggat acctcgaccg gcgtcccgtg gcagacgacc
     7801 gacccgtggt tcacttcgaa ccagtagatg agggacgcat ccttccgggc ttgcagctga
     7861 gtaatccccc gagccatttc ggctccccgg cgatccaacg agtccaagac ggggttttct
     7921 tcccgaaagg aaagcaaccg ttctcccatt cgctccaggg ctcgaaccac cttgccgcaa
     7981 actcggtcaa atccctcccc cacccggggg gaatccaaga gaaaccgacc gccggagcct
     8041 gcgaaaccct gcatggtgtg ctgaaaatcc cgggtcaagg cctcgagatg ctgggcggtc
     8101 ttcaggaggt ctcggacctt ttcccccttg gggagaacct tccggcattc ccttgccaaa
     8161 tccagggcga gctccctcag tccccgactg ctgaattgga ctccgaaata caggcccacg
     8221 atgtcttcca gttgatgagc ttcgtcaaag acgaccccgt cgtagtcggg cagaacttcc
     8281 gtcagggcct tctttctcag ggcgaggtcc gcgaagaaga ggtggtggtt caccaccacg
     8341 agctgggcct gggccgcttc catccgcagc cgatgaacga agcaccggga ttgatcttcg
     8401 cactgggatg ccaggcagtg ttcggtgccg gaacaaacct cgctccaggc ctggaactgg
     8461 tccggaagcc agggaatctc ggcacgatcc ccgctcttgg tgcgaccggc ccactgttga
     8521 aaccgtcgga agagcttggc ttcttcccgg ctgtgaaagg cgggttgata cgcaaactcg
     8581 gaaaagcggc gcctgcagag gtagtttccc cttcctttga gacacaccgc ctgcagccgt
     8641 ggaaaaagaa gtttcttgag aagaggaatg tcctgatcca ggatctggtc ctggagggtc
     8701 ttggtgcccg tggaaacgac aatctttttc ccggtgagga tggcgggaac gagatacgcc
     8761 caggtctttc ccgtacccgt ccccgcctcc accatcaagg gcacttccgt cttcaggcag
     8821 tgatagacgg tctgggccat ctccagctgg gagggccgat attcaaaatg ttcaagcttt
     8881 tgggcgagga ttccctcggg gccgaaatag cgctgcattc atcccggttt caaaaaaggt
     8941 ccgatttgaa aaaggattcc gcggaggaag agggcggtgg cgattccccg gaatctcggg
     9001 caaccttggt cgaggtcggc ggcgccccgg caaatgcggc caaaaccccc ctcgaaccct
     9061 cggggttctc ctcggttccc gcgctctggc ccacgcgggc aaacacgatc cccgggggga
     9121 tgggaaaggc aagaatcggc tcgtttttca ggtattcttc catgaagtac acccagatgg
     9181 gacaggcggc gcgcccgccg gtttctccct ttcccagggg aatggtgtgg tcgtcgtagc
     9241 cgatccagac tcccgccagg atgttgggag tgtacccgat gaaccaggcg tccttgagct
     9301 cgttggtggt gccggtcttt cccgccgcgg gcctacccag cttcttggcc ctctgccccg
     9361 ttccctcctc gatcaccccc tggagaagat ggttcatgag gtaggcggtt tgaggagaaa
     9421 tcacgctttc tcgacgcacc tggcgctcct ccagcaatcg accgtcccgg tcatagattc
     9481 gatcgatgag ggtcggctcc acccgctctc ccaagttggc gaaggccgaa taggccgtga
     9541 gcatctccat gagtgtgacc ccggaggatc ccaaagccag ggccagggtc ggcgtgaggg
     9601 gcgaggtaat gcccagcttt cgggcatagt cgatggcata atggaccccg atatcgtcca
     9661 ggagttttac ggtcaccacg tttcgggaat ggatgagggc tttgcgcaaa aggatgggcc
     9721 cccaaaactg gcggtcgtag ttgctgggtg cccaaacccc tctcaggctg tgatccggtc
     9781 gcacgaaggg cgaatcgatc aggacggacg cctccgtgta ccccttgtcc aaagcggcgg
     9841 cataaatgat gggtttgaag gaagaaccgg gctgacgcag ggattgggtg catcgattga
     9901 attggctctt ctcgaagtcc cttcccccca ccatgctgac gactcgcccc gtttccactt
     9961 ccatggcgag caaagcccct tccatgcccg ggtcctgttc cagggccgca agccaggtcc
    10021 ggccgtcctg ctgcttgtcc agccgcacgc gaatcacatc ccccgggcga aacatctttt
    10081 ccgctctcgt gaccgacaag ccggcccatt gccaaccggt tgcgggcagc tttccttgcg
    10141 aggatcccag gtgaagcagg caggtcttgg ccttggggtc gtaggacagg accagggccc
    10201 ccaccacttt gccgggaccc aattccccgt tcctggccgc caattgggtc agatactggg
    10261 gccagtcatc cttggggatg ttctcatgaa gcccgcggta acgtctgtga cgtttgtcga
    10321 gctcccgaag cccctgatcc agggcctttt tggcaatctg ctgtgcccgg ggatccaggc
    10381 tggtgtgaat cctgagaccc tccttgtaga ggatttccct cccgtagcgg gcctccacct
    10441 gtctgcggac ctcttcggta aaatggtcca gctctttcaa ggtccaatgt ttgggtttta
    10501 ccaggtccag gggggcgttg aaggcctgtc gcgcctcatc ctgcgtgatg aatcccacct
    10561 ccgtcattcg ctgaagcaca tactgctgtc tttcccgggc gacggagtac ttcacgaaag
    10621 gggagtatcg gctgggggct ttgggcaaac ccgccagaag ggcgatctca ttaagtttca
    10681 agtccttggc atgcttatcg aaataggttc gtgccgcggc ctcgatcccg taagcgcccg
    10741 acccaaaata gatctggttc aggtagagat agaggatttc gtccttggtg aggtaatgat
    10801 cgatgcggta agccagaatg gcttctttga gcttgcggat ccaggtgcgt tcgggagtca
    10861 gcaggaggga tttcaccacc tgctgggtga tggtgctgcc cccctgaacg atctctccgg
    10921 cctggatgtt cttgaacatg gcccggatga tccccatgag gtccaccccg ccgtgttcgt
    10981 aaaacctcac gtcttcaacg gcaagaaacg cctggaccag ggccttgggc aactcctcca
    11041 aaggcaccag gtatcttcgt tcggcaaaaa actcgccgat gagcgttccg tccgaggcgt
    11101 aaacgctgga aacaagggga ggatggtagt ttttcaggga ggtgatgttg ggaaggctgc
    11161 gggtcagctg gagatagaag ccccccagga tcgccgtcac ccccaggacc aggcagaaac
    11221 ccaatcccaa caacgtaaaa accaaccagc gaaaggagct cttcttggga actcgtctcg
    11281 aagagtccga gtggttcata gttctcacct catgcacaat ggaacttctt tgtcgagacc
    11341 ccgtaagaat cctcgaagtg ggatggtgtg gccaacggtg cagtctccaa cggatgacaa
    11401 atgactataa accatgaggc gtccgttggc aaataccgga aataggagaa gttcttgatc
    11461 ctcgaggaat cgtctttatc ttctgtggaa gcccgatgag gactcaaaag gaaccggtgc
    11521 ccacgggggg caccggtctt cgagacggac agaaatcaac gatctcaaga agcaggcgat
    11581 tatgaaggca cttcatttca ttacacacgg cggaccggaa gtcttgcgat acggggacgt
    11641 gccggaaccg gtgccgggac cccggcaggt tcgagtgcgg gtccgggctt gcgccgtcaa
    11701 tcacctggac gtctgggttc gccggggctg gcccggcctg gatctctcaa tgccccattg
    11761 gggcggttcc gacatcgcgg gggttgtgga ttccgtggga agcgaggtca cacaatggcg
    11821 cgtaggggat cgggtggttg cgaacccggg ctggtgcccc gtggaggacg aattcactct
    11881 ccggggggag cactccatga gcccccagta ccgcatcctg ggggaacaag tccccggagg
    11941 atttgccgag tacgccctgg tgccggccgg aaaccttgcg gccatgccgg actccctcga
    12001 ctttcccgaa gccgccgccc ccctcgtggt caccatcacg gcctggagaa tgttgatcca
    12061 ccgggccggg cttcgggccg gggagtccgt cctgatcgtg ggggcggggg gaggagtcaa
    12121 taccatggcc gtccagattg cccggctcgc gggagcaacg gtctacgtgg tggccggagg
    12181 cccggagaag gcgaggaaag ccgaggaact gggtgcggac tacgtcatcg accgatccct
    12241 tcgggattgg gacaaggaga tgctcaaact cacccggcgc aggggagtcg acgttgtggt
    12301 ggacaatgtg ggagcggcca ccctgccgac cagcatgaag gtggcggcgc gaggggggcg
    12361 gatcgtcatc gtgggaaata ccagcggccc cctggcggaa ctggacctgc gatacatctt
    12421 cagcaagcag atccaactca tcggcagcac cttgggcaat caccaagatt tcctggaagt
    12481 cctggcactc ctgtggaagg gaaagctcag gccggtcatc gaccgggtga tgcccctgag
    12541 ccggggccgc gaagcgcttc agcggatcga acacggcgat cccttcggca agatcgtcct
    12601 ggttccctga tcttcaagag ggtccccggg ccccggcggg cccctctact cctcttcggt
    12661 ggccaggccg tacttgatga ggcgataccg gaaggatcgg aagctgatgg acaggagttc
    12721 cgcggccttg aatttgactc cctgggtttg ttgcagggcc tgctggagga tcttcttctc
    12781 aatttccgcg aggtattcgt ccaggttcat gccttcgggc ggaatggtgg gccggccggg
    12841 caaaagggag tccgtccgga tcttcttgta ggtagccaag gtgagactgt cgggaaggac
    12901 gatgctggag gattccaggg ccacccctcg ctcgatgatg tgttccagtt cccgaacatt
    12961 gcccggaaaa tcgtagtggt tcaggatatc catggcatag gaggatatct ttcgaatgtc
    13021 tttgccgaac tgcctgcaaa acttgttcag aaagtgctgg gccaggatgg gcacatcctc
    13081 cccccggtcc ctcaaggggg ggatgcgaat gggcaggaca ttcaaccggt agaagaggtc
    13141 ctcgcgaaac gtcccctcga tgaccatctt ttcgagatcc cggttggtgg cggagatgat
    13201 tcggacatcg acggagatat ccacggtatc cccgatcatc ttgatggtct tgtcctggac
    13261 aaggcgcaag agcttgacct ggagcaccgg agacaattct ccgatttcat ccagaaacag
    13321 agtccctccc tgggccgctt ccaccagtcc ttttcggctg gttgtagccc ccgtgaaggc
    13381 tcctttccga tatccgaaga gctcgctttc gatcagggat tcgggaacgc ctccgcagtt
    13441 gaccaccaca aacggttggt cccgccgggg gctctgccga tggatggcct tggccacgag
    13501 ttcttttccc gtgccgcttt ccccggtgac gaggacgttg ctcgaggtcg ccgccacgcg
    13561 ttggatcagc tcatagacct tgcgcatggc cgggctttcc ccgatgaggc agccaaagtg
    13621 cagggggccg tcgccgggcc gggccgggcg atcctcttcc cccccgcggg tctcgagggc
    13681 attgcggatc accgctcgca tctcttcgat gttgaagggt ttgggaaggt agtcgtaggc
    13741 gccttcgttc atggcagcca cggccgtttc cgtgctggcg taggcggaaa tgatgatgac
    13801 caccgtctga ggagacactc tcttgcaccg cttgaggacc tcgagccccc ccaccggttc
    13861 cattcgaatg tcggtgatca ccagatcgaa gaaattcttc cggatcaagt cgagggcttc
    13921 cttcccgttg gccgccgttt ggacccaata gccgtccttc cgaagcatga tttccagaaa
    13981 ttcccgcatg cttcgttcgt cgtcgaccac gagaattttc acctggctgg ccgggagttt
    14041 gctcatgaat ccagtcggga gtaggcgacg atgccgcgga caaaggtcat gacggcccgt
    14101 ccttgtaagt ccttgcccag gaaggggcaa ttgcagcctt tggatcgaag ggtctccgga
    14161 gtgagccggt agatctcgtc cggatccaga atggtcatat ccaccggatt tccccgggca
    14221 agggtgccca ggggcagccc gaggatccgg gcgggattcg tggagacctt ggcgacggcg
    14281 tccgaggggc tcaacgtccc ttccctcacc aactcgagga tgaggggaag tgcggattcg
    14341 aggccgatga tcccgttggc cgcaaattca aactcgatgt ccttttcgat cacgctgtgg
    14401 ggggcatgat ccgttgcgat ggtatcgatg gttccgtcgg ccagggccgt ttttacggcc
    14461 tgaacgtcct ccgtccggcg aaggggaggg ttgaccttga agaccgggtc gaacgagagg
    14521 atcgcttcgt ccgtcaaggt gaagtagtgg ggagcggtct ccgcggtcac catcacgcct
    14581 cgtctcttgg cctcccgaat cagcctcacg gacccggcgc tgctcacgtg cgcgatgtgg
    14641 atgcggctgc gggtccattc cgccaggagc aggtcccggg caaccatggc ttcttcggcg
    14701 acgccgggga tcccgcgaag tcccagaagg gttgaaacgg ggccctcgtt catgagccct
    14761 ccctcggaaa gttgaaggtc ctcggcatga ctgatgacca gcaagtcgaa cgccccggca
    14821 tactccaacg cccgtctcat catgaggctg ttcatgaccg gccgaccgtc atcgctaacg
    14881 gctacggccc ccgcgtcctt caattcccca aactccgaca ggttctcccc cagcagtccc
    14941 ttgctgatgg acccgacggg aaagaccctg caggagcctt cctcccttgc tctttggaga
    15001 atgaattggg tgacggccgc gcaatcgttt accggagagg tgttgggcat gcacgccacc
    15061 gaggtgtacc ctccggccac ggcggccgag gttccgctgg caatggtttc cttgtactcc
    15121 tgcccgggct ccctcaggtg gacatgcata tcgatcagcc cgggcacgat ccatttgccc
    15181 gaaacatcga tttccctgac ctcagcgcct ttgtccgccc ccagttcgat gcggggccga
    15241 atctccgcca gctgcccgtt caggatcaac acatcggcac agaggtcgat ttcatgcgtg
    15301 ggatcaatga cgcgtccgcc ccgaaacagg tagctgcccg ggcctttctc cctctccatt
    15361 ccacaagctc cttcgaatcc ttcgtacccc cgggacttcc cgggaatggg tgcgggagga
    15421 aacagacatt cttccccccg aatcccgcct ctaccgttgt cccagcaggt aaagcagcgc
    15481 cattcgcaca gccacgccgt tggtgacctg gtccaggatc accgaacggg acccgtcggc
    15541 cacctcggga ctgatttcca ccccccggtt gacggggccc gggtgcatga tgaggacctc
    15601 cttcttcgcc ttctccatcc ttgaggagtt gattccatag taaatggaat actcccgcag
    15661 agaggggagc agcatctgct gcatccgctc cttttgaagt cggaggacca tcagcacatc
    15721 ggcttccgga atcgcttcct cgatccgccg gcacacctgc acccccatgg aatccatgtc
    15781 cacgggcatg agggtcgggg gagcgcaaac aatcacctgc gcgcccatct tgcgaagacc
    15841 ccagatgttg gacctggcca cccggctgtg ggcaatgtcc cccacaatga ccaccgtgag
    15901 gccctccagc ttccctttgt gctctcgaat ggtcaacatg tccaggaggg cctgactggg
    15961 atgctcgtgc atgccgtccc ccgcgttgat caccgaggcc gccgtatgct cggcaaggcg
    16021 gtggggagta cccgaagccg ggtgacggat gatgaatacg tccggtttca tggcctccag
    16081 gtttttcagg gtatccagaa aggtttcccc cttgaccagg gagctcgtgg aagcagcaat
    16141 gctgtaagtg tccgcgctga gccgttttgc agcgatgtca aaggaggtgc gggtgcgggt
    16201 gctgggttcg tagaaaagat gaatgatcgt cttgccgcga agagtgggaa ctttcttgat
    16261 gggcctgagg ttgatctcct tgagggatgc ggccaggttc aaaacgtgtt cgatctcgtc
    16321 tgcctccagt tcgcggatcc ccaatacatc ttttcttttg agcgacatga atcatcctcg
    16381 tcaaggtggg tggtcggaat catcccctta tcctcgctac tctagcacga aaccggatcc
    16441 ccgttccaac aaattcgagg gaaccccctt cggtccccgg gcaaggaccg tgcttccacc
    16501 gcacatctgc cgcggggtcc cacggaccct cccgggcaaa acctctccgg ttgagcttga
    16561 cagaaattca tgagccaatt aaatgtaagc atttgatccg tggaggaatg gaatgcaaag
    16621 agtgcccatc accaaggacg gcttcgagag gctgaaaacc gaattgcagc ggctccagaa
    16681 agaggagcgg cccgtcatca tccgtgccat cgaggaggcc agaggacacg gagatatcag
    16741 tgagaacgcc gagtatgaag ccgccaaaga aaaacaaggc ctcatagagc gacgcatcct
    16801 ggatctcact caaaagctca gcgagtgcca gatcatcgaa ccctcggacg agggcaacgg
    16861 gaaggtcgtc ttcgggagca ccgtggtcat ggaagacctg gagacccatg aggtgacgac
    16921 ttatcgcctg ttgggtccct acgaggccga tgtgggctcc ggcaccattt ccgtgacctc
    16981 tcccctggga aaggcgttga tcggaaagga agagggggaa gaggtccggg tacagacccc
    17041 aaaggggatc cgcaacttcg aaatcttgga agtcctttat tgaaggtgcc gacccctccg
    17101 ttttccgccg accctcgttt ttccgttgat gccccgtagg cccctgagta cgggaaaacc
    17161 gtcgtttttg ccggccaagg aatgtgctgt tcgaggaacc aatgagtagg attcgcctca
    17221 ttcatttcga aataggaggg atcggcgatg aggtgtaaat cgatggtggc ggttgccgcc
    17281 ttttttcccg ccgttctttg gctggcccca atggcaatgg gtgctccctt ttgccggcag
    17341 aaggacttcg agggagtatg gaacgtgtac gccatgggct ccggagaatg gggtccctgg
    17401 tggcaggcat gcaagatggt cgtggccaga gacggcatga
//
LOCUS       DAOOVC010000080        17165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2273_length_17165_cov_7.230742, whole genome shotgun sequence.
ACCESSION   DAOOVC010000080 DAOOVC010000000
VERSION     DAOOVC010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2273_length_17165_cov_7.230742"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1370
                     /locus_tag="PLM79_10980"
     CDS             81..1370
                     /locus_tag="PLM79_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HOV86878.1"
                     /translation="MPEDLKKMYRTVMEDHFPDSLRIFFGDQELRYRKRRWKFPDEKS
                     GELIEKGLRYGENPGQEAALYELVGGHLELGKCRFIEPDRGLVSAIREEDMLQEGKHP
                     GKINLTDLDNGLNILKFLMGKPAAVILKHNNPCGAAHGESLARAYDRANRADRIAAFG
                     GCVVFNRPVDKETARLVAQNYLEVVAAPDYEEGTLEILKARSNLRIIQVARIDRLADY
                     VPWRFVDFKSLTDGGLIVQQSPLNRVRDGRDLKLAEAAHDGKLYRIRRGPSEQEIRDL
                     LFGWFVEQGVTSNSVLYVKEECTVGIGTGEQDRVGVAEIAVFKAYRKYADALCFDRYG
                     ISYKDLELAVEKGQRPKEEKLEIDRETQEAKGGLPGSTMVSDAFFPFRDGVDVGLRQG
                     ITAVIQPGGSMRDWEVVEACNEAGATMVFTGQRAFKH"
     gene            1654..5340
                     /locus_tag="PLM79_10985"
     CDS             1654..5340
                     /locus_tag="PLM79_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV86879.1"
                     /translation="MRKKGGCFFSLLSFLWIAGAGQVWSQAPGILSNPPSIPCGTEYP
                     LLLPGFLLLALGVFVAAFLVQRSRRLSAERQVRESEERYREIFENAVEGRFRTTLDGR
                     TLSVNPAFARMFGYSSPREMIESGKDLDREAHFRPRDHGPLIELLRERGEVRDFELET
                     CRKDGSRFWMSVTARTVLDPAGTLQCIEGTCVDITERKHAEEALRRSQQFIESILNTI
                     PVRVFWKDKDLKYLGCNAIFARDAGCRAPEDLVGKDDFEIPWHHQADRYRKDDLSVLE
                     SGRPKLLIEEPQTTPSGDLIHLLTSKTPLRNPDGEIIGILGAYLDITERKKAEAALEE
                     SERRFKLVLETLPVGFWLVDKEGRLLMANRAGERIWGGSLVAEPEEYGTLRAWRLPGR
                     EPVPAKDRALHRALHRGETTSEELLEIETFDGQRKFVLSWASPVESPSGEILGAFLIH
                     QDVTESVRVTEALRESEEKYRAIFENATEGIFQNTVEGKYLKVNPAFARMFGFSSPRE
                     MVDAVSDIAEQLWVNPQERRRMVEVLRERHRVEGWEAEVYRRDKSRFWISINAHTVRD
                     SSGNILYLEGTNVDITARKLAEEAALQAQQEWSATFDAMNDAVALIDLDGRILKCNAT
                     MARLLERAPSEIIGFRCHTLVHGTGTPIPECPLRRAIRSGSRETMDLAVGDRWFRVSA
                     DPFFGVNGEMAAMVHIMTDITESKRAEQKLKESEAAYRTFFEQSPLSVTLNRMNGEFV
                     EVNQTFCRTFGRGAWEVMGKTPVELGIIDEKTLETMAEILRREGGVLTGFEWSSTLPD
                     GRLMHAVTTAKTVEMKGETMVLSLIQDITERKSADEQQKRLKEQLFQAQKLEAVGRLA
                     GGIAHDFNNILSAIMGYCELARLELSAETRVACDLDRVLEACERARDLIAQISTFTRR
                     GEEESHPMLVIPVVKEALKLLRASIPSTIEIRRSIPAEDIIVKADPTRVHQIVMNLCT
                     NAYHAMMKDGGVLHVSAEEIDLGEQPSAPDADLPSSPCFVLTVRDSGQGIPPEILDKV
                     FDPYFTTKQKDLGTGLGLAVVKGIAEQLGGRVEVSSELGKGSTFRVFLPSIKIPVLES
                     DSRETVMERGSERILFVDDEKNIAEIGKRMLEELGYRVFAFSDSVEALDFFHRDPEQV
                     DLVVTDYTMPLMTGDKLAAAILEVRPDVPVILCTGYRKGFSEEQAAAVGIARLVPKPL
                     AMASFSKLIRSVLDERARVGAQDG"
     gene            5333..6748
                     /locus_tag="PLM79_10990"
     CDS             5333..6748
                     /locus_tag="PLM79_10990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86880.1"
                     /translation="MARVLILDDETHVLAVLQELMVSMGHEPVCCSSLDKGVRIAREQ
                     IFDLIFLDLDFPNGNGLDVLPTLVHAPYSPQVLIITGAGTRNAAEIAFRHGAWDFVRK
                     PFNLGEVKVYTSRALQYRDERLASAAPRFLKRDSIIGSSPVLQSCLEELARAAFGDAS
                     VLITGETGVGKELFARALQENSPRSSQPMIVADCGAIPETLAESIFFGHEKGAFTGAG
                     QARQGLIKQAHGGTLFLDEIGEMPLALQTRLLRVLQERRFRPIGSHQEVESDFRLVAA
                     TNQDVDLMVREGRLRQDLLFRIRAIQIRLPPLRERKEDIPALVTHHLVRLGKLYGTDI
                     KGVSAEFLNALLVYDWPGNVRELVHAVERAFSAAMDTGTIHPFHLPPEIRARSVFQGL
                     EKEPGKPGMNMEPPDLPGMLEFPTLKDYLDAAEKHYLLALMQRVAGRREEAVRLSGLS
                     QSKLYQALKQHAIPGFRGSRT"
     gene            7120..7548
                     /locus_tag="PLM79_10995"
     CDS             7120..7548
                     /locus_tag="PLM79_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86881.1"
                     /translation="MRVLLYAGVEGEVASRLVSCLAENASTSTLEVHFTPERFIPRLL
                     KIPRDFHTVLVIAGKEELARLSGVWDHCLPAKLILILKEWEDELLGVALRLAPSFMLS
                     TGHELREVGLVLAKIEMDRRRKCRAAVGGVPGIRWGPYAH"
     gene            complement(8250..8843)
                     /locus_tag="PLM79_11000"
     CDS             complement(8250..8843)
                     /locus_tag="PLM79_11000"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HOV86882.1"
                     /translation="MSSKTLIVAVTGASGALYAKLLLRHLWEQGVPVHLIPSGAGKLV
                     YQLEVGTPLEEDLPPTVRCFDNKDFSAPPASGSVSYHGMVIVPCTMGTLAAVANGLSL
                     NLIHRAADVCLKERRPLVVVPRETPLNRVHLANMLRLAEAGGIILPAMPGFYHHPRSL
                     EDVGRFIVARILDQLGFPQDLVPPWNPEPEIFPGIAS"
     gene            complement(8869..9672)
                     /locus_tag="PLM79_11005"
     CDS             complement(8869..9672)
                     /locus_tag="PLM79_11005"
                     /inference="COORDINATES: protein motif:HMM:NF005556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate
                     synthase"
                     /protein_id="HOV86883.1"
                     /translation="MSGKALRLKRLFSKGDGRLVVFPLDHGVTFGPIPGLRQLAGVIG
                     MGVRAGVDAFVLHKGAMAFLETIPPPLPAVLMHLSASTGIGASIRRKVLVGSVEEALC
                     RGADGVSVHINLGGEHEPEMLRDLGSVGAACQRWQVPLLVMIYIRGIHAPSPVPDTAV
                     AHGARVAAELGADVIKIPLPDDVELLAEIANSFPVPVIMAGGSKEPDPRLFLGRVEQA
                     LDAGFSGVAVGRNVFQNEKPQCLLRCICSMVHKRMNAQGAWDEWAGELF"
     gene            complement(9731..10591)
                     /locus_tag="PLM79_11010"
     CDS             complement(9731..10591)
                     /locus_tag="PLM79_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOV86884.1"
                     /translation="MGPSPPTGLKLGKIGFLNVLPIHYPLERGILPHPFQIVSGAPAH
                     LNELMARGDLDLSVVSSIEYARHHERYYILPNLSISCRGPVRSVVLMSRVPVGELSGK
                     KVLVSAESHTSVALLRILFRFRLGVQAEFEVGNCSRELSHERYPAAFLAIGDEALRLR
                     THALYPHMLDLGEAWHSWTGLPFVFALWILQRDAVERWNGQLEPALDTLIRAKRWGAD
                     HLDSVCREASRRGVLGYRGLYEYYRCLRYDLGDEELEGLRLFYRFLMDIGEIGAEPPL
                     DLFSSLAGAA"
     gene            complement(10564..11862)
                     /locus_tag="PLM79_11015"
     CDS             complement(10564..11862)
                     /locus_tag="PLM79_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV86885.1"
                     /translation="MDLQPGPIRGTRLRTGEVPRSPVLHRAAWVMTMEEPPLCDGAVL
                     TQSGRIVAVGAFRALRSRCPAQTTVRDHPDCALLPALVNAHTHLELTGLEGQIALPQP
                     GFARWLEELMPLRRALSAQSQKTALEDGERRLFQEATALCADITNGATLACRDSFSGT
                     ERTPILEVIGFDRPDLESALGLEVRASLDAAVGAGTCGGLGAHACYSTSPPVIREINE
                     ATRTHGLLFSIHVAEHEDEIEFLQTGTGACRDLLEKLGKWIPGWEPPGSTPIQYLDDL
                     GVLDERTLLVHCVHLTRQDWQIVASRGCSVCFCPRSNRNLNVGRAEIARAAERNIPWA
                     LGTDSLASNRDLSLFAEACAAMESNPQVSPDEFLRAVTRGGARALHVQQDYGSIRPGK
                     RDALIAVSLPASLRKAPLSETILYRGNEGEWTWVHHPRPD"
     gene            complement(12041..13099)
                     /gene="mqnC"
                     /locus_tag="PLM79_11020"
     CDS             complement(12041..13099)
                     /gene="mqnC"
                     /locus_tag="PLM79_11020"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOV86886.1"
                     /translation="MLTERSGPSDRSRRLTFPEAADLYANADFYELGRRAHEVRMRKH
                     PEPVVTYVVDRNINYSNVCVCGCRFCAFFRPPDHPEAYVLSRQELGAKIEETLRLGGT
                     QILLQGGHHPLLPLSFYEEMLAFIKERYPIHIHAFSPPEIVHFARLEGMGIPEVLGRL
                     RAAGLDSIPGGGAEILVDSVRRKVSPGKCTAGEWLNVMEEAHAQGLRTTATMMFGHEE
                     GPVHRLEHLFALRELQDRTGGFTAFIPWAFQPTNTAISRFPETAVSYLRLLAVSRLVL
                     DNFDNVQASWVTMGPKIAQLALQFGANDFGSTMIEENVVAAAGVHFRMPVDEIRRVIR
                     ACGFQPRQRTMDYGRVTG"
     gene            complement(13115..14218)
                     /gene="mqnE"
                     /locus_tag="PLM79_11025"
     CDS             complement(13115..14218)
                     /gene="mqnE"
                     /locus_tag="PLM79_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOV86887.1"
                     /translation="MLDSEIYRKLGLADIREKVADGGRLSLEDGQRLFDCPDILAVGS
                     LAHHVRTRLHGNAAFYVLNQHINYSNVCVNGCRFCAFGRKKGDPLAFQMSVEEVLDKV
                     RSRLGEPITEIHIVGGCHPDLPFSYYEEMLAGVRALRPSARIKAFTAVEIAHFARLGG
                     LSVREVLVRLRASGLDMLPGGGAEIFSPRVRQLLCPTKISGPEWLELSRQAHGLGIQT
                     NATMLFGHLETTRERLEHLVAVRELQDETRGFICFIPLPFQTANTGISDIEPTTGIDE
                     LKTLAVSRLMLDNFPHVKAYWIMLTVKLAQVALHFGADDFDGTVMEERIGHMAGAQSE
                     QALTRSRLERIIVEAGFEPVERNCFFEPVSRNP"
     gene            complement(14293..15003)
                     /locus_tag="PLM79_11030"
     CDS             complement(14293..15003)
                     /locus_tag="PLM79_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV86888.1"
                     /translation="MDLPKTFSRLENDKQQRILDTAIEEFARHGFRQASLNRVVQRLG
                     IAKGSIFQYFGNKEGLFQVIFGHAVEMVRRSLRQVKQETAESDFFERIRKSLLAGISF
                     IDQHPRIYQIYLKMIFQEDFPLRTAFLQQVHLFSAEYLRPMVQRGLDRGELRQDLDPD
                     MTVFFLDALMDRFLQAYCVSFLDAGAGLYQAPPDQLDKKVKEFIRLLREGIGRPPHGE
                     PRREPDPPPMGDPDKRTP"
     gene            15066..15860
                     /locus_tag="PLM79_11035"
     CDS             15066..15860
                     /locus_tag="PLM79_11035"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86889.1"
                     /translation="MSTNQTPPQTRVALGKRLRSCPGIPCHGVKPNWKDYSLEVRRAV
                     QRADLVCYPSTLYEDVLNSLGRRTFPRNYYRFMGNKIQQTHLFQLLEVPHPRTRLYYG
                     RNRAQRILEDFSPPFVAKDPVGSSQGLGVWLVTCREDLDRYLVGHHPAYIQEYLPIDR
                     DLRVVLVKGKAVHAYWRAGREGEFRHNVSRGGRISFEEVPPEAVSFAEEVGRRCGFEE
                     VGLDICRFEERFYVLEANMVFGLEGFRAMGLDISRILCDRVLRENP"
     gene            complement(16220..>17165)
                     /locus_tag="PLM79_11040"
     CDS             complement(16220..>17165)
                     /locus_tag="PLM79_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86890.1"
                     /translation="LEKNLDTLVRIDPGRASFKEHLSQARIHAEELAHALHKYAQQIT
                     FDPQRLSAVEERLALLHRLGKKYGGSVEAMLRRTEELRKELNEAEDVGMQEEELQNAL
                     EEARRRYLEEAGRLSRSRREAADRLAAEVVGTLARLDMPSVRFRALFSPEDVPTDPGE
                     DLFSPRGIDQVEFLLSANLGEDLKPLSKVASGGELSRILLSLKGLLGRKGDAETLIFD
                     EVDAGIGGRTAELVGLQLRRLARKQQVICITHLPQIACCGDGHYRVAKETVGQETLTG
                     IRRLSPEERIEELARMLGGIDISEKARAHAAEMLKSLR"
BASE COUNT         3473 a         5135 c         5278 g         3279 t
ORIGIN      
        1 agttttgatt tgatagaagc gtacgggtat cataaaaagg tctggagaaa atccgggcct
       61 ttttttatgg agggacgatt atgccagaag acttgaagaa gatgtatcga acggtcatgg
      121 aagaccattt ccccgattcc ctgcgtattt tcttcgggga ccaggaactt aggtatcgca
      181 agcgaagatg gaaattcccc gacgagaaat cgggggaact catcgaaaag ggtttgcgat
      241 acggagagaa tccgggacag gaagcagcgc tgtacgagtt ggtgggcgga cacctggaac
      301 tggggaaatg ccgcttcatc gagcccgacc ggggactggt cagcgccatc cgggaagagg
      361 acatgctcca ggagggaaag catccgggaa agatcaacct caccgacctg gacaacggcc
      421 tcaatatttt gaaattcctc atggggaaac ccgctgcggt catcctcaag cacaacaacc
      481 cctgcggagc cgctcacggg gagtccctgg cccgggccta cgatcgggcc aaccgcgcgg
      541 atcgcatcgc cgcctttggg ggctgtgtgg tgttcaaccg gccggtggat aaggaaacgg
      601 cccggctcgt ggcccaaaat tatctcgagg tcgtggccgc cccggactac gaggaaggca
      661 ccctggagat tctcaaggcc cggtccaacc ttcggattat ccaggtggcg aggattgatc
      721 gcctggcgga ttatgtgccc tggaggttcg tggatttcaa atccctgacg gacgggggcc
      781 tgattgtgca acagtcgccc ctcaaccgcg ttcgagacgg ccgggatctg aaattggccg
      841 aagccgccca cgacgggaag ctctaccgca tccgtcgggg accctcggag caggaaattc
      901 gggacctgct cttcggatgg ttcgtggaac agggagtcac ttccaattcc gtgctctacg
      961 tgaaagaaga atgcaccgtg gggatcggca ccggcgagca ggaccgggtg ggggttgcgg
     1021 agatcgccgt cttcaaagcc taccggaaat acgcggatgc cctctgtttc gaccggtacg
     1081 gaatctcgta caaagacctg gaactggcgg tggaaaaagg ccaaaggccc aaagaagaaa
     1141 agctggaaat cgaccgcgag actcaagaag ccaaaggggg actgccggga tccaccatgg
     1201 tgtccgatgc ctttttccca tttcgggacg gagtggacgt gggcctgcgc cagggcatca
     1261 ccgccgtgat acagccgggg ggttccatga gggactggga ggtcgtggaa gcctgcaacg
     1321 aagccggcgc caccatggtg ttcacgggac agagggcatt caaacattga gggcctgagc
     1381 cggttcccgg atctttggca ggccatcttc tgccggaatg cccttctttt cctgcgtttt
     1441 cgaccttttc gtcccgagag ggccgctcgg aggcgggcgg tcccggggag cctccaacgg
     1501 gttgcggccc cgaaggtttt gtctcggccc tcacgattcc acggacaaag gtcccaccaa
     1561 ggacccgccc atggggcggc cgtcggccga gacttatcga ctggtgtata ggttcgcggg
     1621 cgttagtctc tttcgagaag aaggacgagg gccatgcgga agaagggcgg ctgctttttc
     1681 tctctattgt ctttcctgtg gatcgccggg gccgggcaag tctggtccca ggcccccggc
     1741 atcctttcca acccgccttc aatcccctgc gggaccgaat acccccttct cctcccgggg
     1801 ttcctgctcc ttgccctggg tgtttttgtc gccgcattcc tggttcagag gagtcgcagg
     1861 ctctctgcgg aacgacaggt gcgggaaagc gaggagagat accgggagat cttcgagaat
     1921 gccgtcgaag gcaggtttcg gacgaccctc gatggacgaa ccctcagcgt caatcccgct
     1981 tttgcccgga tgttcgggta ttcctccccc cgggagatga tcgaatcggg taaggatctc
     2041 gaccgggagg ctcatttccg tccccgggat cacggcccct tgatcgagtt gctcagggaa
     2101 cggggcgaag ttcgggattt cgagctggag acctgtcgca aagacgggag caggttttgg
     2161 atgtccgtca ccgcacgcac ggtcctcgat cctgcgggca cccttcaatg catcgaaggc
     2221 acctgcgtgg acatcaccga gcgaaagcat gccgaggaag cgttgcggcg ctcccagcag
     2281 ttcattgaga gcatcctcaa cacgatccca gtacgggtct tttggaagga caaggacctg
     2341 aaatacctgg gctgcaacgc gatcttcgcc cgagacgccg gttgccgggc ccccgaggac
     2401 ctggtgggca aggacgattt cgagattcca tggcatcacc aggcggatcg gtacagaaag
     2461 gacgaccttt cggtcctaga aagcggacgc cccaagctcc tgatcgagga accccaaacc
     2521 accccgagcg gcgacctcat tcatctcttg accagcaaga cccccctgag gaatccggac
     2581 ggagagatca tcggcatcct gggagcctac ctggacatca ccgaacgtaa gaaggccgaa
     2641 gcggccctcg aggaaagtga gagacgattc aagctggtcc tggagaccct tcccgtcggc
     2701 ttctggctgg tggacaagga gggcaggctc ctcatggcca accgggcggg ggagcggatc
     2761 tggggaggaa gtctcgtggc ggaaccggaa gaatacggga ccctccgggc ttggcgtctc
     2821 ccgggccgcg aacccgtccc ggcaaaagat cgggcgctac accgggcgct acaccgggga
     2881 gagacgacct cggaagagct tctcgagatc gagaccttcg acggacaaag gaaattcgtt
     2941 ctcagctggg cctcccccgt tgaaagccct tccggagaga tcctgggagc cttcctgatc
     3001 caccaggacg tgacggaatc cgtccgggtg accgaagcgc ttcgggagag cgaagaaaag
     3061 tatcgagcca ttttcgaaaa cgccaccgag gggatttttc agaataccgt ggaaggcaag
     3121 tacctgaagg tcaatcccgc ctttgcccgg atgttcggct tttcatcccc ccgggaaatg
     3181 gtggatgcgg tttccgacat cgccgagcaa ctatgggtga atccccaaga gcgccgccgg
     3241 atggtggagg tgctccggga acgccacagg gtcgaagggt gggaagcgga agtctatcgc
     3301 agggacaaga gccggttttg gatctccatc aacgcccata cggtgcggga ttcgtcaggg
     3361 aatatcctct atctcgaagg caccaatgtc gacatcacgg cccgcaaact tgccgaagag
     3421 gcggctctcc aggcccaaca ggagtggtcg gccacctttg atgccatgaa cgatgccgtt
     3481 gccctgatcg acttggatgg ccgcatcctg aaatgcaacg cgaccatggc ccggttgctg
     3541 gagcgggccc cttcggaaat catcggtttc aggtgccata ccctggtcca cggaacgggg
     3601 accccgatcc ccgaatgtcc cctacgtcgc gcaatccgtt ccggaagcag ggaaaccatg
     3661 gatctcgccg tgggagaccg ctggtttcgc gtgagcgcag atcccttttt cggagtcaac
     3721 ggcgaaatgg cggccatggt gcacatcatg accgacatca ccgagtccaa gagagcagaa
     3781 cagaagctca aggagagtga ggcggcctat cgaaccttct tcgaacagag ccctctgtcc
     3841 gtcaccctga accggatgaa cggagaattc gtggaagtga atcaaacctt ctgcaggacc
     3901 tttggacgcg gtgcttggga ggtgatgggc aagaccccgg tggaactggg gatcatcgat
     3961 gaaaagaccc tcgaaaccat ggctgaaatc ctgagaaggg agggaggcgt cctgacggga
     4021 ttcgagtgga gctccaccct gcccgacggc cgcttgatgc atgcggttac cacggccaag
     4081 accgtcgaaa tgaagggtga aaccatggtg ctctctctca ttcaggacat caccgagcgc
     4141 aaatctgcgg atgaacagca aaagaggctg aaagaacagc tcttccaggc ccaaaaactc
     4201 gaggccgtgg gtcgtttggc cggggggatt gcgcatgatt tcaacaacat tttgtcggcc
     4261 atcatgggct attgtgagct ggcgcgcctc gagctgtctg cggagacccg ggtcgcttgc
     4321 gatctggacc gggtcctgga agcatgcgag agagccaggg acctcatcgc gcagatctcg
     4381 accttcaccc ggagaggtga ggaagagtcg caccccatgc tcgtcatccc cgtggttaag
     4441 gaggccctga agcttcttcg ggcgtccatc ccttccacta tcgagatccg ccggtccatc
     4501 ccggcggagg acatcattgt gaaagcggac cccacccggg ttcaccaaat cgttatgaac
     4561 ctgtgcacca acgcctatca cgccatgatg aaagacggcg gggtcctcca cgtgagtgcg
     4621 gaggaaatcg atctgggaga gcaaccttcg gcgcctgacg cggatttgcc ctcgtcgccc
     4681 tgcttcgtcc tcaccgttcg agactccggt cagggcattc ctcccgagat tcttgacaag
     4741 gtgttcgacc cctacttcac caccaagcaa aaagacttgg gaacaggcct gggtcttgcc
     4801 gtggtcaagg gaatcgcgga acagttggga ggcagggtcg aggtctccag cgaattggga
     4861 aaggggtcca ccttccgtgt tttcctgccc agcatcaaga tcccggtgtt ggagagcgat
     4921 tcgcgggaga cggtcatgga gcgcggcagt gagcgaatcc tgttcgtgga cgatgaaaaa
     4981 aacattgcgg agatcggcaa aaggatgctc gaggaactcg gataccgggt tttcgccttc
     5041 tccgacagtg tggaggccct ggattttttc caccgggacc cggaacaggt tgaccttgtc
     5101 gttacagact atacaatgcc cttgatgacc ggggacaagt tggcggcggc gatcctcgag
     5161 gtgcggccgg acgtgcccgt cattctctgc acggggtaca ggaaagggtt ttccgaagaa
     5221 caggcggcgg ccgtcggaat tgcccggctc gttcccaagc ccctggccat ggcctccttt
     5281 tccaagctga tccgaagcgt gctggacgaa cgtgcccgtg ttggagccca agatggctag
     5341 agtcctgatc ctagacgacg aaacgcatgt cctggccgtg cttcaggagt tgatggtcag
     5401 catggggcac gagccggtat gctgcagctc cctggacaaa ggagtccgaa tcgcccggga
     5461 acagattttt gacctcatct ttctcgatct tgattttccc aacggcaacg ggttggacgt
     5521 gctgcccacc ctggtacatg caccctattc gccccaggtg ctcatcatta cgggagcggg
     5581 gacccggaat gccgccgaga tcgcctttcg gcacggggcc tgggattttg tgagaaagcc
     5641 cttcaacctg ggagaggtca aggtctacac ctcgagggcg cttcaatacc gtgatgaaag
     5701 actcgcaagt gcagcccccc ggttcctgaa acgggattcc atcatcggaa gctcgccggt
     5761 ccttcaaagc tgcctggaag agttggctcg ggctgccttc ggagacgcca gcgttctcat
     5821 taccggagag acgggggtcg gaaaggagct cttcgcccga gccctccagg aaaacagtcc
     5881 ccgatcctcc cagccgatga tcgtggccga ctgcggtgcc atccccgaaa ccctcgccga
     5941 gagcatcttt ttcggccatg aaaagggagc gttcacggga gcgggccagg ctcgccaggg
     6001 tctcatcaag caggcccacg gcgggaccct ctttctggat gagatcggag aaatgccctt
     6061 ggctctccag acaagactgt tgagagtctt gcaggagagg cggttcagac ccatcggctc
     6121 ccaccaggaa gtcgaaagcg attttcgcct ggttgcggcc acgaaccagg acgtggattt
     6181 gatggtccgg gaaggtcgcc tgagacagga tctgttgttt cgcatccggg ccattcaaat
     6241 ccggctcccc cccttgaggg agagaaaaga ggatattccc gccttggtca ctcaccacct
     6301 ggttcgactc ggcaagctct acggaacgga catcaaggga gtttccgccg aatttctcaa
     6361 cgcgctcctc gtctacgatt ggccggggaa cgtgcgggaa ctggttcatg ccgtcgagag
     6421 ggctttttcc gctgccatgg ataccggcac catccatccc ttccatctgc ctcccgagat
     6481 acgggcgaga agtgtctttc aaggcctcga aaaggaaccc gggaaacccg ggatgaatat
     6541 ggaacctccc gaccttcccg ggatgctcga attcccaacc ctcaaagact accttgacgc
     6601 cgcggaaaaa cactatctgt tggctctcat gcaaagggtt gcggggcgaa gagaagaagc
     6661 cgtgagactt tcaggactgt ctcagtccaa gctctaccag gccctcaagc agcacgccat
     6721 tcccggcttt cggggatccc gaacctgaag tccccaacga ccggggctcc ccctccatga
     6781 attctcccga tctgcgtctt caacaccccg aattccaatt ccaaggaaga ttctttccac
     6841 cagcgaagaa atctctgtgc tccggcggag gctccgtgtg ccgtcccccc ctgagaaatc
     6901 actaactcct ggtttttcct tgtataaatc gaagtccccc cacccggaat cgatcggcac
     6961 gttccttgcc acacggagcc ggggaaggag tgcgttcctg atcacaaatg agcccaaggc
     7021 tccgtcccga gacggggtct gccggggatc gaggacggag agcaaatgcg ggagctcaaa
     7081 acaagggtct gtgaacattc cgaaccggag attcccgaca tgagggtgct tctctatgcc
     7141 ggtgtcgaag gggaggttgc gtcccggttg gtttcctgcc tcgcagaaaa tgcgtcgacg
     7201 agcactctcg aggttcattt tacccccgaa agattcatcc cgcggctgct gaagatcccc
     7261 cgggatttcc acacggtcct ggtgattgcg ggaaaggagg agttggctcg gttgagcggg
     7321 gtgtgggacc attgtttgcc cgcaaagctc atcctgatcc tcaaggaatg ggaagatgaa
     7381 ctgctgggtg tcgccctccg gcttgctccc tctttcatgt tgtctaccgg gcacgagttg
     7441 cgtgaagtcg ggttggtgct ggccaagatc gagatggacc ggagaaggaa gtgccgggcc
     7501 gcagtgggtg gcgtaccggg aatccggtgg ggcccctacg cccattgaag tcgactggat
     7561 gagcttacga accggggttt tgggaaagag gccgactttc caggcagagg tgaagacctg
     7621 cacggacgca cgacgccatt gcgccgcgtc gggcatgact tgaagaagac gcactggtga
     7681 aaggatggga cgggagcatg cgcgattcgt ccgggaatcc gggtgccggc cacggcagat
     7741 acctcatgtt cggtctcaac aatgaagaat acggcgtggc cgtcctgaag gtgagggaga
     7801 tcgtcgggat gttgccgctg aggccgattc cccgaacacc tccattcgta aagggggtaa
     7861 tcaacctacg gggcggaatc gtccccgtga tcgacctcaa actgtgcttg ggactgaaag
     7921 cggagagggg caacggccgt acctgcatca tcgtgctgga cttgagcgtg gaagactcgc
     7981 tcgttgccgt cgggatcctc gtggactggg tctcggaggt gaaacacatc ggggcgaatc
     8041 aagtgcagag tccgccgttg ttgggcatgg gcccccgagc tcgcttcatc gaggggatct
     8101 acatgaccga gggaggggtc aggatactcc tcgatatcga gaaagtcctc cgtgacgaga
     8161 gtcttgccgt cccgggccct tcagagtggg cacggcagca gactcttgcc cgggaaagcg
     8221 agcaatgcgg aagagaaccc gggtttttgt caggaagcga tcccggggaa gatctcgggt
     8281 tcagggttcc agggtgggac caggtcctgt ggaaatccca gctggtcgag aatccgggcc
     8341 acaatgaacc ggccgacgtc ttcgagactc cggggatgat ggtaaaaacc gggcatggcc
     8401 gggagaatga tgccccccgc ctccgcaagc cgtagcatat ttgccaggtg aactcgattg
     8461 agaggcgtct cacggggtac caccaccagg ggccttcgtt cttttagaca gacatccgcc
     8521 gcccggtgga taagattcag ggacaggccg ttggcgacgg ccgccagggt ccccatggtg
     8581 cagggaacga tcaccattcc atgataggaa accgatccgc ttgccggagg agcggaaaag
     8641 tccttgttgt cgaagcaccg tacggtcggc ggcaagtcct cctcgagggg ggtccccacc
     8701 tccagttggt aaacgagctt tcccgcaccg gacggaatca aatgaaccgg aactccctgc
     8761 tcccagaggt gcctcagcag gagtttcgca tagagggccc cgctggcccc ggttacggca
     8821 acgataaggg tctttgagga caagcccccc tccctttcca ccgttttctc aaaaaagttc
     8881 cccggcccat tcatcccaag ccccctgagc gttcatcctc ttgtggacca tggagcagat
     8941 gcatcgaagg aggcactggg gtttctcgtt ctggaagacg tttctcccaa cggcaacccc
     9001 gctgaatccc gcatcgagag cctgctcgac cctcccgagg aaaagccgag gatcgggttc
     9061 cttgcttcca ccggccatga tgacgggaac cggaaaggag ttcgcgattt cggcgagcaa
     9121 ttccacatcg tcgggcaagg gaatcttgat cacgtcggcg cccagttccg cggctacccg
     9181 ggctccatgg gccactgccg tgtcgggtac cggagagggc gcgtgaattc cccgaatgta
     9241 gatcatgacc aggagaggaa cctgccatcg ctggcaggcc gctccgacgc tgcccaagtc
     9301 ccggagcatt tcgggctcgt gttctcctcc caggttgatg tggacggaaa ccccgtcggc
     9361 cccgcggcac agggcttcct cgacactccc caccaacacc tttcggcgaa tcgaggcgcc
     9421 gatcccggtg cttgcggaaa ggtgcatcaa gacggcgggg aggggcggag ggatcgtttc
     9481 caggaaggcc atcgccccct tgtgcaggac gaaggcgtcc acccccgccc ggacccccat
     9541 gccgatgact cccgccaact gtcgcaaccc ggggatcggc ccgaaggtga ctccgtgatc
     9601 cagggggaat accaccagtc gcccgtctcc cttggagaaa agccgcttca gcctcaaggc
     9661 ctttccgctc atttgcttgt ctcccgaaga atcccgctgc gtctcttccg tcggctcacc
     9721 ccgggggcaa tcaagccgcc cccgccagcg aggaaaacaa gtccagggga ggctccgccc
     9781 cgatctctcc gatgtccatc aggaaccggt agaagagccg gagtccctcc aattcttcgt
     9841 ctcccagatc atatctcagg catcgataat attcataaag tccccggtag ccgagcaccc
     9901 cccggcggga tgcttcgcgg cacacggaat ccaggtggtc cgcgccccac ctcttggcac
     9961 ggatgagcgt atccagcgca ggctccagtt ggccgttcca tcgttccacg gcgtcacgct
    10021 ggaggatcca gagggcaaac acaaagggga gccccgtcca ggagtgccac gcctccccca
    10081 ggtccagcat gtggggataa agggcgtggg tgcgaagcct cagggcctcg tctccaatgg
    10141 caagaaacgc cgcgggatac cgttcatgag acagctcccg gctgcaattt cccacctcga
    10201 attccgcctg gacccccaaa cgaaaacgga acaggatgcg aagcaatgcc acggacgtgt
    10261 gggactcggc actgaccagg actttctttc ccgaaagctc tccaacgggt acgcggctca
    10321 taagcaccac gctcctcacc ggccctcggc aactgatgga caggttggga agaatgtaat
    10381 agcgctcgtg atgccgggca tattcgatgg aggacacgac actcagatcc aggtcccccc
    10441 gggccatgag ctcgttgagg tgagcaggag ctccggaaac gatttgaaag ggatgtggaa
    10501 ggatcccgcg ctccagggga tagtggatgg gcaggacgtt caaaaacccg atttttccca
    10561 gcttcagtcc ggtcgggggt gatggaccca tgtccattct ccttcgtttc ctcggtacag
    10621 gatggtttca ctcagtggag ctttccgcag ggatgcagga agggagaccg caatcagcgc
    10681 gtccctcttt ccagggcgga tcgatccgta gtcctgctga acatgaagag cccgggcgcc
    10741 tccccgggta acggccctca agaactcgtc gggggaaacc tgcgggttgg attccattgc
    10801 cgcgcaggct tcggcaaaca ggctcaggtc ccggttgctg gcaagactgt ccgtgcccag
    10861 ggcccaggga atgttccgct cggcagcccg ggcgatttcc gcccggccga cgttgagatt
    10921 ccggttgctt cggggacaga aacacacgga acaaccccgt gacgcaacga tttgccaatc
    10981 ctgccgggtg aggtgtacgc agtgcaccag aagggtcctt tcatccagga cccccaggtc
    11041 gtcgagatac tggatggggg tgctcccggg aggctcccag ccgggaatcc acttgcccag
    11101 tttttcgaga agatcccgac aagcgccggt ccccgtctgc agaaactcga tctcgtcttc
    11161 atgttccgcc acatggatgg aaaatagaag gccgtgggtc cgagtcgcct cgttgatctc
    11221 tcggatgacg ggtggcgacg tggagtagca cgcatgagcc cccagtcctc cgcaggttcc
    11281 cgccccgacg gccgcatcca gggatgctcg aacttccagg cccagggctg attccaggtc
    11341 cggtcgatcg aaaccgatga cttcgaggat gggcgttctt tccgtccctg aaaagctatc
    11401 ccggcaagcc agggtggctc cgttggtgat gtccgcacaa agggccgtcg cctcctggaa
    11461 aaggcgccgc tctccgtcct ccagggccgt tttctgggat tgggccgaaa gggcgcggcg
    11521 caaaggcatg agttcctcga gccaccgggc gaaacccggt tgcggcaacg caatttgtcc
    11581 ttcaagccct gtgagctcga gatgggtgtg ggcgttcacc aatgcgggca atagagcgca
    11641 atcagggtgg tcgcgaaccg tcgtttgggc cggacaccgg gatcggagtg cccgaaaagc
    11701 gcccacggcg acgatccggc ccgactgggt caggaccgct ccgtcgcaca agggaggctc
    11761 ctccatggtc atcacccagg cggcccgatg caggacgggc gaacggggaa cttctccggt
    11821 ccggagcctt gtcccgcgta ttgggcctgg ttggagatcc atgaatcctc ccgtacattc
    11881 gttcttccac ggaatttccc gggaaaaaga aaagtcccgg gagccctcta cccgaagaca
    11941 acccgtcccc ttccccagcc tccccgcccg gggggcttcg cttcgctccg cccaccccac
    12001 accgctccgc ccaccctgca tcgcctcccg gcttgtcggc ctatccggtc acccgcccat
    12061 aatccatggt cctttggcgg ggttggaacc cgcaggcccg gatcaccctt cgaatttcgt
    12121 ccacgggcat ccggaaatgg acccccgccg ccgccacgac gttttcctcg atcatggtgg
    12181 atccgaaatc gttggctcca aattgcaggg cgagctgggc gatcttgggt cccatggtga
    12241 cccaggacgc ctggacgttg tcgaaattgt ccagcaccag gcgcgatacg gccagcagcc
    12301 tcaggtaaga gaccgccgtt tccggaaacc gggagatggc ggtgttggtg ggttgaaagg
    12361 cccacgggat gaaggccgta aagccccccg tgcgatcctg caactcccgg agggcaaaga
    12421 gatgctccag ccggtggacg ggtccttctt cgtgaccgaa catcatggtg gccgtggtcc
    12481 gcagcccctg ggcgtgggcc tcttccatga cgttcaacca ttcgccggcc gtgcatttcc
    12541 cgggggagac cttacgtcga accgaatcca ccaggatttc cgctccccct ccggggatgg
    12601 aatccaggcc cgcggccctc agccgtccca ggacttccgg aattcccatg ccttcgagcc
    12661 gggcgaagtg aacgatctcc gggggagaaa aggcatgaat gtggatgggg tagcgttcct
    12721 tgatgaaggc cagcatttcc tcgtagaagg aaagtggcag caacgggtgg tggcctccct
    12781 ggagcagaat ctgggttccc cccaggcgaa gggtttcttc aatcttggct cccagttcct
    12841 gccgggacaa cacataggct tccgggtgat cgggaggccg aaaaaaggcg cagaagcggc
    12901 atccgcacac gcagacgttg gaatagttga tgttgcggtc caccacgtag gtcaccacgg
    12961 gttccgggtg tttccgcatg cggacctcgt gggcccgacg ccccagttcg tagaaatcgg
    13021 cgtttgcata gagatccgcg gcctcgggga acgtgaggcg gcggcttcga tcgctgggtc
    13081 ctgaccgctc cgtcaacatg ttcggggtgc cttctcaagg attccgggag accggttcga
    13141 agaagcagtt gcgctcgacg ggctcgaatc cggcctcgac aatgatccgc tccagccggg
    13201 agcgcgtgag ggcctgttcc gattgggccc ccgccatatg cccgatccgc tcctccatca
    13261 ccgttccgtc aaagtcgtcg gccccaaagt gcagagccac ctgggccagc ttgacggtga
    13321 gcatgatcca gtaggctttg acgtggggga agttgtccag catcaggcgg ctgacggcca
    13381 aggtcttgag ctcgtctatg ccggtggtgg gttcgatgtc cgaaatcccc gtgttggccg
    13441 tctggaacgg cagcggaatg aagcagatga acccccgggt ttcatcctgg agctctcgaa
    13501 cggccaccag gtgttcgagc ctttcccggg tggtttccag gtgtccaaag agcatggtcg
    13561 cattggtttg gatcccgaga ccgtgggcct ggcgactgag ctccagccat tcagggccgc
    13621 tgatctttgt gggacaaagc agctgacgca cccgggggct gaagatttcg gcgcctccgc
    13681 ccggcagcat gtcgagcccc gaggcacgaa ggcggaccag gacctcccgg acggaaagcc
    13741 ctcccagcct ggcgaaatgg gcgatctcga cggcggtgaa ggccttgatc cgggccgagg
    13801 gccgcaacgc tctcaccccg gccagcatct cttcatagta agaaaacggc aaatccgggt
    13861 ggcacccccc aacgatgtga atctccgtta tgggctctcc gaggcgggac cggaccttgt
    13921 ccagcacctc ctcgacggac atctggaagg cgagcgggtc cccctttttg cgtccgaacg
    13981 cgcaaaagcg acagccgttg acgcagacgt tcgagtaatt gatgtgctgg ttaagaacgt
    14041 aaaaggcggc gttcccatgg agccgggtgc gcacgtggtg agccaaagag cccacggcga
    14101 ggatgtcggg gcagtcgaag agtctctgcc cgtcctccaa gctcaaacgc ccgccgtccg
    14161 cgaccttttc acggatgtcc gccaagccca gtttccggta gatttccgag tccaacatga
    14221 gggaccgtcc ttttcctgga agcctgccac ccaaccacgg catcggacct gccgggggaa
    14281 gtcggttcct tttcaggggg ttcgcttgtc gggatctccc atcggtggag ggtccggttc
    14341 ccgccgaggt tcaccgtggg gaggcctgcc gattccctcc cggagcagcc gaatgaactc
    14401 cttcaccttc ttgtccagct ggtcgggagg agcttgatac aagcccgccc ccgcatcgag
    14461 gaaggaaacg cagtatgcct gaagaaagcg gtccatgagg gcgtccagga agaacaccgt
    14521 catgtccggg tccaggtcct gccgaagttc cccgcgatcc agcccccttt gcaccatggg
    14581 cctcaggtat tcggccgaga acaaatgaac ctgttgcaga aatgccgtgc gtaggggaaa
    14641 atcctcctgg aaaatcattt tgaggtagat ctggtagatt cgcgggtgct ggtcaatgaa
    14701 cgagatcccg gccagcaggc tcttgcggat tcgttcgaaa aaatccgatt ccgccgtttc
    14761 ctgcttgacc tgccgcagag agcgtcgaac catttcgacg gcatgaccga atatgacctg
    14821 gaagaggcct tccttgttgc cgaagtattg gaagatggaa cccttggcga tccccagccg
    14881 ctggaccacg cgattgaggc tggcctgccg gaaaccgtgc cgggcgaact cctcgatggc
    14941 ggtgtccagg atgcgctgct gcttgtcgtt ttccaacctg ctaaaggttt ttgggaggtc
    15001 catggcaagc acacttcttg ggttccaaaa atgaccaccc ggtcattata ggagaattcg
    15061 cccgaatgtc aacgaatcaa actccgccac aaacccgggt ggccttggga aaacgcctcc
    15121 gatcctgtcc gggaattccg tgccacgggg tgaagcccaa ctggaaagat tactccctcg
    15181 aggtccgtcg ggccgtccaa agggcggacc tggtctgtta ccccagcacc ctctatgaag
    15241 atgtgttgaa ttccctggga agaaggacct ttccccgaaa ttattaccgg ttcatgggaa
    15301 acaaaattca gcaaactcac ctgtttcagc tcctggaggt cccccaccca aggacccgtc
    15361 tctactatgg ccgaaaccgg gctcagcgca tcctcgagga cttctcccct ccctttgtcg
    15421 ccaaggaccc cgtgggctcc tcccagggac tgggggtctg gctggtgaca tgccgcgaag
    15481 acctggaccg ctaccttgtc gggcatcatc cggcctatat ccaggaatat ctccccatag
    15541 accgggacct gcgggtggtg ctcgtcaaag ggaaagcggt ccatgcctac tggagagcgg
    15601 gacgggaggg cgaattccgc cacaatgtct cccgcggggg gcgcatatcc ttcgaagagg
    15661 tcccgccgga ggccgtaagt tttgccgaag aggtcggtcg acggtgcgga ttcgaggagg
    15721 tgggcctgga catctgccgg tttgaagaaa ggttctacgt tctcgaggcc aacatggtgt
    15781 ttgggctcga agggtttcgc gcgatggggc tggacatctc ccgaatcctc tgcgaccggg
    15841 tcctgaggga gaacccttga accgcattcc ggcctgcgtc ccggcacccg ctggtcgacc
    15901 cgggcttcgg ctttcggggt tgttcggacc cggtcgagga cccaaaccgg gcgatcggtt
    15961 gccgttgtac ggcttcaacg ggcgggcaca aagcccacca ctacggtgac gggggaacgg
    16021 gttgtgcagg ggcggggccc cttccccgcc ctccgggatc gccggcaggc gcaacctgca
    16081 aatccctttg gacacccggt ttatccccgc cggcagggac cgccgtcgga tggggcatga
    16141 ccggcaagca tcccccggtt gggcggaaat tcctccggtg cctttcccga ggggatttgt
    16201 tgcggcgggc agatccaggt cagcggaggg atttcagcat ttcggccgca tgagcccggg
    16261 ctttctcgga aatgtcgatg ccccccagca tccgggcgag ttcctcgatg cgctcttccg
    16321 gggagaggcg acgaatgccc gtgagggttt cctgccctac ggtttccttg gccacccggt
    16381 agtggccgtc cccgcagcat gcgatctggg gcaggtgggt gatgcagatg acttgctgtt
    16441 tccgggccag gcgccggagc tgcaacccta ccagctccgc cgtgcggccg ccgattccgg
    16501 catccacttc gtcgaatatg agggtttccg catctccttt tcggccgagg agacccttca
    16561 aggaaagaag aatcctcgaa agttcgcccc ccgaagccac cttggacaaa ggtttcaggt
    16621 cttcgcccaa gttggccgaa agcaggaatt ccacttgatc gatcccccgg ggggaaaaga
    16681 ggtcttcccc ggggtcggtg gggacatctt ccggggaaaa gagggctcgg aacctcaccg
    16741 agggcatatc cagccgggcc agggttccca ccacttcggc ggccagtcgg tcggccgcct
    16801 cccgcctcga tcgggacaac ctgcctgctt cctcaaggta tcggcggcgt gcttcctcga
    16861 gggcattctg cagctcttcc tcctgcatgc cgacgtcttc cgcctcgttc agctcctttc
    16921 ggagctcctc cgtgcggcgg agcatggctt cgacgctgcc tccgtatttc tttcccaggc
    16981 gatggaggag ggcaagtcgt tcctccacgg ccgagagcct ctgggggtcg aacgtgatct
    17041 gctgggcgta cttgtgaaga gcatgggcca attcctcggc gtggattcgg gcctgggaca
    17101 gatgctcctt gaaagatgcc cggcccggat cgattcggac cagggtgtcg agattcttct
    17161 cgagg
//
LOCUS       DAOOVC010000081        16781 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2328_length_16781_cov_6.261031, whole genome shotgun sequence.
ACCESSION   DAOOVC010000081 DAOOVC010000000
VERSION     DAOOVC010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16781
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2328_length_16781_cov_6.261031"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            589..1800
                     /locus_tag="PLM79_11045"
     CDS             589..1800
                     /locus_tag="PLM79_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HOV86891.1"
                     /translation="MQHRGEIQDADRHTSPGSEAEADPSVPFYTELPQVREESLPLPE
                     AILFPDQGPEQAQSTSVVVAQAQRQAWRRWEDGYALTSGSPQWDKLIAELASEIKTRH
                     YSRKTLKTYAHWSRRFQGFLKNKPPGSVSSKDVKEYLTYLAVQCRVSASTQNQAFNAL
                     LFLFRHALKRDFGDFKDVPRAKKSKYIPVVLSRKEIDTVLKHLQSPYDLVVKLLYGCG
                     LRLFECLQLRVKDFNLEARILTVHGKGKKDRTVPLPETIVPELTAQIELVKKVHDEDL
                     RAGYAGVFLDDRLEKKYPSAAKDFVWQWFFPQKTLTQVSSTQELRRYHLHETHVQGAL
                     YEAVRRARLTKRVTSHTFRHSFATHLLQANYDIRTIQELLGHSDVKTTMIYTHCIPSK
                     TAKEAKSPLDF"
     gene            complement(2475..3620)
                     /locus_tag="PLM79_11050"
     CDS             complement(2475..3620)
                     /locus_tag="PLM79_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOV86892.1"
                     /translation="MSRSANPGRPCLLQGNEAIVEGALAAGCRFFAGYPITPATEISE
                     GMSARLPALDGTFIQMEDEIAGLGAVIGASLAGAKAMTATSGPGFSLMQENLGFACIA
                     EVPCVIVNVMRGGPSTGLPTHVSQGDVMQARWGTHGDHPIIVLAVSTTWDCFHGTVRA
                     FNLSEKYRTPVIILADEVVAHTREKIFLPDPREIEVLERIRPSVPPEWYIPYEDNSRG
                     VPDMGVFGDGYRYHVTGLVHDVRGFPTERPDEITALVSRLHRKINQHFPDIQTVDFFL
                     ADDAEVLVIAYGSVARSARSAVIEARERGVKAGLAKLVTLWPFPRQILEPFLRRVRAV
                     LVPELNMGQMSREVKRVNQGMTWVETLNRIDGNLITPKEILARLLKL"
     gene            complement(3739..4047)
                     /locus_tag="PLM79_11055"
     CDS             complement(3739..4047)
                     /locus_tag="PLM79_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86893.1"
                     /translation="MAKKEAGNRRPSKVAPASPQGSEPGEEGDKKRFDIDIFRDWCKS
                     CGICAAFCPRECIALDENGSPVIDRGDRCTGCGWCELHCPDFAISVRPRRAKKPPEED
                     "
     gene            complement(4440..5447)
                     /locus_tag="PLM79_11060"
     CDS             complement(4440..5447)
                     /locus_tag="PLM79_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOV86894.1"
                     /translation="MDSHSLILTILIVLVALSFDFINGFHDSANSIATVVSTRVLSPQ
                     SAVVWASFFNFTAIFIAGIPVAKTIGTGIVQPGVVNNFLVLAALGGAIAWNLITWWLG
                     LPSSSSHALIGGLMGAGFVQGGTAVLQWNGILKTAAFIVLSPAIGLVLGLGIMIVVLN
                     LSVRSNVFKTDRVYRRLQLFSAAVYSVSHGMNDAQKTMGIIAMVLYTGGYLGESFYVP
                     FWVVFLCYLFIALGTMAGGWRIVKTMGSRITRLEPIGGFAAETAAAASIIGASMAGIP
                     VSTTHTITGAIVGVGAVRRLTAVRWGVAGSIVWAWILTIPLSAAISALLFLGFKALHL
                     C"
     gene            complement(5440..6060)
                     /locus_tag="PLM79_11065"
     CDS             complement(5440..6060)
                     /locus_tag="PLM79_11065"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOV86895.1"
                     /translation="MRLIPKNEKFFDHFEELGALIEKGGLLFLDMLNHYEHSEAKLPT
                     LKEIEHDADRVTHIIYSKLHQSYITPLDREDIHALARKMDSILDLIEASAIRMHLYKV
                     KNPTREIIELAVILNNAIGLVLKVVRGLRHKKDHPMVMEWLVEINTLENEGDYILRQA
                     MARLFERETDPFDLIKLKEITELVEEAIDTCEDVSNIVEGIILKHG"
     gene            complement(6242..6967)
                     /locus_tag="PLM79_11070"
     CDS             complement(6242..6967)
                     /locus_tag="PLM79_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOV86896.1"
                     /translation="MVTLPIAHTDRTYTLNPSKIIALGLNYRDHIAESGSVKVRGFTD
                     EIPSEPILFPKTPNVLIGPEEPIVLPAFLEGYGFEKLRTDYEAELAIVIGKRCKNLDP
                     RDARGVVFGYTCMNDVSQRNLQTADRSGWFRGKSLDTFGPIGPCIVLPEDLGDPQELG
                     LQCRLNGRVVQDSNTRYMIFPIPEILAFVSKNFTLMPGDVIATGTPSGVGPIEHGDVV
                     EVEIQGIGTLRNPVRSESAGSRP"
     gene            complement(7011..7811)
                     /locus_tag="PLM79_11075"
     CDS             complement(7011..7811)
                     /locus_tag="PLM79_11075"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOV86897.1"
                     /translation="MSRSKGRNVPDPAEPDRAPKRHRVEFSTKHVFTGLFALVLVLAW
                     MFMLGVLAGRGLPLIDSEDLSLRAHLVRFIGLDKEVAPERPDAAETWDSPKKMLETLS
                     YYEDLAQKESPPAPETDQPAPAAPQPRNSSKTSPAEKTPATPDASKAGGKDKAAPPKT
                     AKETPKEATKEAAKEAVKEAPPPEIAAGHFTLLIASLRDAENAQKLVEQLKSKGYPSR
                     LEPLDLQESGRWNRILVGSFQSREEALRFAADFNRKERLEGLVIREPH"
     gene            complement(7836..9512)
                     /gene="argS"
                     /locus_tag="PLM79_11080"
     CDS             complement(7836..9512)
                     /gene="argS"
                     /locus_tag="PLM79_11080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOV86898.1"
                     /translation="MGIVRKRVEETLSRAVARVLDSPTLPDSLSIELDIPKFSQHGHY
                     ASNAALSLSRILKRPPREIASRIIENLESDPGMIRKVEIAGPGFINFFIQPAAWLPIL
                     SQIHREGKTYGTQTVGKGRKVQVEFVSANPTGPLHIGHGRGAAIGDVLANILEVCGYE
                     VQREYYINDAGNQMNTLGSSLYYRYRELLGKEVAFPEGHYQGDYMRDLARDFLEKEGE
                     KYRDSAMDEVLPVFARFAGDRILEGIRQDLASFGVTFQEWFSEQTLHDRGEILGTIEA
                     LEKLGFVYEAEGAKWFRSTAFGDEKDRVVVRANGISTYFAADLAYHKNKFERGFHMVV
                     DIWGADHHGYVDRMSAGVQALGRDRADLRIILVQLVNLLRGGKPVAMSTRAGEFVTLR
                     EVIDEVGKDAARFIFLTRRTDSPLDFDLDVAKMQTNDNPVFYVQYAHARLSSLFEVAG
                     QRGISCRWAEGGEEPPLHRLTVPQELEILQQLGEYPQVLLNCVKSLEPHYIPYYLHEL
                     VSLFHSYYNRNRILGDDPELTQARLYLASALRRVIGNALGVLGVAAPEKM"
     gene            complement(9593..10720)
                     /gene="alr"
                     /locus_tag="PLM79_11085"
     CDS             complement(9593..10720)
                     /gene="alr"
                     /locus_tag="PLM79_11085"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOV86899.1"
                     /translation="MPSSWIEIDLGALRHNYQQVCRRLHPATRVLGVVKSDAYGHGMV
                     PVARELEKSGVHFLAVSKFWEARELRSAGIRVPILVLLGLEPGEMEEAVRLQVRPVLF
                     RPDHARELSRAARGRDLPAPFHLKVDTGMGRLGVPWDRTQGFLEEILALPGLELEGVV
                     SHLAVADEADKAYSKIQVSRFRHVLNLLSRKGLEVTYAHISNSAGVLDIPEAHFQLVR
                     PGIMLYGSSPSREILRPADLRPVMTFKCKVLQLKDVPPGSPIGYGRTFVTQRNTRVAT
                     LPVGYDDGYPRLLSNRGAVLVRGRRAPVAGRVSMNMITVDVTGIPDVREDDEAVILGS
                     QGSEQITADEIADLSGTISYEIYCNMGRHACKVFLNGLDSA"
     gene            10850..11053
                     /locus_tag="PLM79_11090"
     CDS             10850..11053
                     /locus_tag="PLM79_11090"
                     /inference="COORDINATES: protein motif:HMM:NF021366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOV86900.1"
                     /translation="MMLKGYLLTVAGLICFFEGLPYLATPDQLKRWLLKVCTLPSSYL
                     RLLGGALMILGLLLVYWGRRLGG"
     gene            11057..12160
                     /gene="queA"
                     /locus_tag="PLM79_11095"
     CDS             11057..12160
                     /gene="queA"
                     /locus_tag="PLM79_11095"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOV86901.1"
                     /translation="MKSRDCLESDPAPSSPGYSLEDYDYPLPESLIAQKPCSRRESSR
                     LLVLDRAGKRVQHRRFDDLARFLDPRDVLVVNDTRVVPARLHGTKETGGHVEMLVLEP
                     FKKPESEARDGYRCLIKSSKHPKEGSVILLGNGARLTVLSPVRDGMARIAFPDTVPLS
                     DLLEEVGEVPLPPYIRRDRPGDFPEDVADYQTVYAQRPGAVAAPTAGLHFSLPLLGEL
                     DRLGVTRVPVTLHVGYGTFSPIRVEDIRLHRMHAEFAEIPPGSAEIIERARREGRRIV
                     GVGTTVVRILEWVCHRFGAVLPFSGYCDHYIIPGYRFHVIDAMVTNFHLPKSTLLLLV
                     SAFAGRDRILEAYREAVRASYRFFSYGDAMLIL"
     gene            12176..13288
                     /gene="tgt"
                     /locus_tag="PLM79_11100"
     CDS             12176..13288
                     /gene="tgt"
                     /locus_tag="PLM79_11100"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOV86902.1"
                     /translation="MNFEILAIDPSCRARRGRLQTRHGTVQTPVFMPVGTQGSVKGVS
                     PDELESLGTEILLGNTYHLYLRPGADRIARLGGLHSFMHWNRSILTDSGGFQVFSLAK
                     IRKIEEEGVVFQSHIDGSRHRITPEGAVRIQEQLGSDIAMCFDECTSYPIPYEYARQS
                     MELSVRWARRCKDAQTHADQALFGIVQGSVFPDLRRECLDRLMEIGFQGYALGSLSVG
                     EPKEAMLAVLEALVPGMPSYAPRYLMGVGTPEDLVEGVHRGVDLFDCVMPTRNARNGM
                     LFTSRGNLQIKNSRYAEDPDPVEPGCTCYTCLRFSRAYLRHLYLSRELLAYRLNTLHN
                     LHYYLSLMHKMREAVEAGTFLQWRRDFYAGREASEI"
     gene            complement(13380..14468)
                     /locus_tag="PLM79_11105"
     CDS             complement(13380..14468)
                     /locus_tag="PLM79_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86903.1"
                     /translation="MAVNVKEIPGANRAAEAANLEWAYRSIVSSSQQKLFRTVLTEAQ
                     SVDDPGTSCGTDSLQADLQSIQVARDRGQNIDAMKQGGVSAPRGGEMNDLATSALLNL
                     AAVTGRRDQLAAYYSQAVASRRAGAATAPGASSASQPLSGRPLPREVGRLGELSARFE
                     SGEMGIKAIGYDEKGGTSYGSYQIASRVGTMRQFLSYLEDHAPEWARRLKAAGPADTG
                     SRQGGMPAEWQKIAEEDPVRFARLQRDFIEESHYFPAMEEIYERTGVNIGKQPRALRE
                     VLWSTAVQHGPRGAANIFCRAIEESRTEGGPGPLRDLVRSIYDSRSTQFSGSNPDIRG
                     SVLRRFREERALALALLAAENRPSEAPA"
     gene            14946..15143
                     /locus_tag="PLM79_11110"
     CDS             14946..15143
                     /locus_tag="PLM79_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86904.1"
                     /translation="MPGKFEIDRSPNGGCCVCSMNERELYLVREASSGRLVEVCSCCL
                     LERVDDFVLDNTRPWPGLERS"
     gene            15799..16194
                     /locus_tag="PLM79_11115"
     CDS             15799..16194
                     /locus_tag="PLM79_11115"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOV86905.1"
                     /translation="MGMIARGRPLKGLDMAEENEEHLAEKEAMVLQDEHYQTVAFLAK
                     SLSDENRLRIILCVSNGKKSVSSIVEELGISQPLVSHHLKELKRSLLVKIERSGPFIY
                     YELVDPRILDVIRTLSTVATDLLTTRKTF"
     gene            16221..16397
                     /locus_tag="PLM79_11120"
     CDS             16221..16397
                     /locus_tag="PLM79_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86906.1"
                     /translation="MEKIRKIVGRMDPEKALEEISEVLKVLFSALGEEARSQFLWDLL
                     GESSGDKVSSLVHL"
     gene            16410..16670
                     /locus_tag="PLM79_11125"
     CDS             16410..16670
                     /locus_tag="PLM79_11125"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOV86907.1"
                     /translation="MGEGVDPTQMCQRLVDKVAQSKQLMTMTDPDLLVLFEDWFEELE
                     EEIVSLVRERGPLDPGELAERTGLSLRGATFLLSKLKREGKL"
BASE COUNT         3253 a         5099 c         4999 g         3430 t
ORIGIN      
        1 tctacatcaa acctgaaaat aatgcacgca tatttgttgc tttttcaaca ccctgataga
       61 tgccagatcg aagccagcaa gtccgacaat ttcctttatg tcgtaaaaag taaaattgtt
      121 ttgcagagtt gctcgaagag cattccatgc ttggtctata tttttcattt tgccctttcg
      181 attatagcgc ctaacaattc attgagtagt ttcctctcaa tgccaggaaa tcttgagagt
      241 gtcaacgtga taatagaatt aaattgacag atctatagga atttattagt taagtcacgt
      301 tgttcaatat cctgttattc ctacaaagga ggtttctgct caatgcagcc tgttccgatt
      361 tcaattgtta ggggctttga gacaattctt gaaaaacgac gcattccggt gactcaacgg
      421 gcaaattaca agaagtggct caggtatttt tgggactttt gcgccaagta tcctgtcccg
      481 gaagcccgat ctgaccaagt gcgtttgttc atcgataagt tgcgggaaaa gaaacagccc
      541 ccgtttcagc aaaatcaggc agcccatgcg atttcccttt attttcagat gcagcacaga
      601 ggcgaaatac aggatgcgga tcgccacacc agtccgggat cggaggccga agcagacccc
      661 agtgtaccat tctatacgga gcttccgcaa gtccgggagg agtctctccc tttgcccgaa
      721 gcgattctct tcccggatca gggaccggag caggcacaga gcacttccgt tgttgttgcg
      781 caggcgcaac ggcaggcctg gcgccgctgg gaagacggct atgcccttac ctcaggctcg
      841 ccccaatggg ataaactgat agccgagctt gcctccgaga tcaagacccg gcattactcc
      901 agaaagacgc ttaagaccta tgctcattgg tcacgccggt tccagggttt tctgaagaac
      961 aaaccgccgg gatcggtatc atccaaagat gtaaaagaat acctgaccta tctggcggtt
     1021 caatgccgtg tctcagcctc cactcagaac caggcgttca atgcgctgct ttttcttttc
     1081 aggcacgctt tgaagcggga tttcggggat ttcaaagacg ttcctcgagc caagaaatcg
     1141 aaatatattc cggtagtcct ttcccgaaaa gaaatcgata ccgtcctgaa gcacctccaa
     1201 tccccttatg atctcgtggt caaattgctt tacggctgcg gactcaggct gttcgaatgt
     1261 ctgcagcttc gggttaagga tttcaacctc gaggcccgaa tcctcacggt ccatgggaaa
     1321 gggaaaaagg accgcacggt ccccctgccg gagacgattg taccggaact gactgcccaa
     1381 attgagctgg tgaagaaggt ccatgatgaa gatctcagag cgggatatgc cggggtcttt
     1441 ctcgatgacc ggcttgagaa gaaatatccc tcggcagcta aagattttgt atggcagtgg
     1501 ttttttccgc agaagacact gacgcaggtt tcctcgacac aagaactgcg gcgataccat
     1561 ctgcacgaga cgcatgttca gggcgcactc tacgaggccg tgcgcagggc caggctcacg
     1621 aaacgcgtca cttcccacac ctttcgtcac agttttgcca cccacctttt gcaggcgaat
     1681 tacgacattc gaaccatcca ggagctcctc ggccacagcg atgtgaagac cacgatgatc
     1741 tacactcatt gcataccaag caagaccgcg aaagaggcga agagccctct cgatttttga
     1801 gagaatgggg tggcaagtcg gtctgtggcg agtaagccat cacccggtgg gtaccccggc
     1861 cgccagaagg tgttggtcag gtccccaatc ccgactttgg tgcataccgc cttcatcccg
     1921 tgctgaatat caacagatcc tctccagcat gccaatcatg ttgggcatct atatcaagag
     1981 ccaccggaag cacctgacgg aaccggaaca cgttctgatg atgcctgaaa cctttcttcc
     2041 gaacggcgtt ggatgtccaa tggcagcgtc tttggcacga ccaaaatcat ccacatcttc
     2101 atgatgaaga ttgcgtacaa ctttgcgttg cgggtgaaat cctgcggtga tcgtttggct
     2161 tcgggactca ttcttttggg gatgaggttg agaaaagctg cgatgacagg ggtgagtcgg
     2221 catgaatata tggcaggacc tgtgttatga tggccaactc ctctgctgac atttgaagga
     2281 tggaaagctt gtttcagcct ccgacaagag acagtatcgc acaagtcggg tgtgattgca
     2341 atgaaatatc ttttcatttc aaagacttat tgcattctcc gtaatgtgcc gatccttcag
     2401 tcaacagcat gggggccggg ggggaggcgg atcggccccg tccggcaaca gccgtttccg
     2461 cggtgaggcc cctcctagag tttcaagagg cgggccagga tttccttggg ggtgatcagg
     2521 ttcccgtcga tgcggttgag ggtttccacc caggtcatcc cctgattgac gcgcttcacc
     2581 tcccgggaca tctgccccat gttcagctcg ggcaccagga cggcccgaac cctgcgcaga
     2641 aagggctcga ggatctgccg cggaaagggc cacagggtca ccagctttgc caggccggcc
     2701 ttcacccccc gctcacgggc ctcgatcacc gcacttcggg ccgaacgggc cacggacccg
     2761 taggcgatca ccagcacctc ggcgtcgtcg gccaggaaga aatcgacggt ctggatgtcg
     2821 ggaaaatgct ggttgatctt tcgatggagc cggctgacca gggccgtgat ttcatcgggc
     2881 cgttccgtgg gaaatcccct tacgtcgtgg accagccccg tcacgtgata gcggtacccg
     2941 tccccgaaca cccccatgtc cgggactccc cggctgttgt cttcgtacgg gatgtaccac
     3001 tcggggggca cgctgggccg gatccgctcc agcacttcga tttcccgcgg atcgggaagg
     3061 aagatcttct cccgggtgtg ggccaccact tcatcggcca ggatgatgac cggggtgcgg
     3121 tatttttccg agagattgaa cgcccgcacc gtaccatgga agcaatccca ggtggtggaa
     3181 accgccagaa cgatgatggg gtggtctccg tgagtccccc atcgggcctg catgacgtcc
     3241 ccctggctca cgtgggtggg aagccccgtg ctgggtcctc cccgcatgac attgacgatg
     3301 acgcagggga cttcggcgat gcatgcgaat cccaggttct cctgcatgag ggaaaacccc
     3361 gggccgctgg ttgccgtcat ggccttcgcc cccgccaggg aggccccgat gacggccccc
     3421 agcccggcaa tctcgtcttc catctggatg aacgtaccgt ccagggcggg cagacgagcc
     3481 gacattccct cactgatctc cgtggccgga gtgatgggat agcccgcaaa aaagcggcac
     3541 cctgcggcaa gagcgccctc gacgatggct tcattgccct gcagcaggca gggacgtcct
     3601 gggtttgcgg aacgagacac ccgttttcct cccggctttt cccggcaacc tctcgattgg
     3661 gtcgccgggt gatgcttacc actgcaaaag tccgtcgaat gggaccggat cgggcgggaa
     3721 aatcccccgg ccggaggttc aatcctcctc cggcggcttt ttcgccctgc gcgggcgaac
     3781 gctgatggca aaatccgggc agtgcagctc gcaccatccg cagcccgtgc agcggtcgcc
     3841 ccggtcgatg acgggagacc cgttttcatc cagggcaatg cattcccggg ggcaaaaggc
     3901 ggcgcaaatg ccgcaggact tgcaccagtc ccgaaatatg tcgatgtcaa accgcttttt
     3961 gtccccttcc tccccgggct cggacccctg tggggaagcg ggagcaactt tggacggtcg
     4021 gcgatttcct gcctctttct tggccatgag aagccccact gtggatttcg gggaatggaa
     4081 cccggaaagc ccgtgttgcc cgactcgggg aaaaaccgcc tcggaccccc cctcctctcc
     4141 gggggagtgg tccgaggagg gcgtcgaaat cccggctatt accggcggcc acccccgatc
     4201 ggcacggacc tcctcctctc cgggggagga ggaaaaccgg gctctcaggc aatcacttag
     4261 cccaaaattc ccgacgtttc aatggagatg ggggcgcccc cccggtcgtc cctaggaccc
     4321 ttgcatgcgc gggtccccgg tagagaccgt ttgcccctgc ggcccgacga ccctttgatg
     4381 cccggtgcac aagcagggtc gaacggcggt ttgcccctgc acccgggacc ggccggggtt
     4441 cagcaaaggt gcagggcttt gaagccgagg aagagcaggg cggagatggc ggccgacagc
     4501 ggaatggtga ggatccatgc ccagacgatg ctcccggcca ccccccaccg cacggcggtg
     4561 agtcgccgca cggcccccac ccccacgatg gctcccgtga tggtgtgggt ggtgctcacg
     4621 ggaattcccg ccatggacgc cccgatgatg gaagcggccg cagcggtttc cgccgcaaaa
     4681 cctccgatgg gctcgagccg ggtgatccgg gatcccatgg ttttcacgat acgccatcct
     4741 cccgccatgg ttcccagggc gatgaacagg tagcacagaa acaccaccca gaagggcacg
     4801 taaaacgatt cccccaggta tccccccgta tagaggacca tggcgatgat gcccatggtc
     4861 ttctgggcgt cgttcatgcc gtgactcacg ctgtagacgg cggcggaaaa caactggagc
     4921 cttcgataca cccggtccgt cttgaacacg ttgctcctga ccgacaggtt cagcaccacg
     4981 atcatgatgc ccagtcccag gaccagcccg atggcggggg agagcacgat gaaggccgcc
     5041 gtcttgagga tcccgttcca ttgaagcacg gcggtccctc cctggacgaa acccgctccc
     5101 atgagccccc cgatgagggc gtgggatgag ctgctgggca ggcccagcca ccaggtgatg
     5161 aggttccacg cgatggctcc ccccaaggcg gccagcacga ggaaattgtt caccaccccc
     5221 ggctggacga tccccgtccc aatggtcttg gccacgggga ttcccgcaat gaagatggcc
     5281 gtgaagttga aaaaggaagc ccacaccacg gcactctggg gcgagagcac ccgggtggac
     5341 acgacggtgg cgatggaatt ggccgaatcg tgaaaaccgt tgatgaaatc gaaggatagg
     5401 gcaacgagga cgatcaggat cgtcaggatc agcgagtgac tatccatgtt tcaggataat
     5461 tccttccacg atgttggaga catcttcaca ggtgtcgatg gcttcttcca ccaactcggt
     5521 gatctctttc agcttgatga ggtcgaaagg gtccgtttcc cgctcgaaaa ggcgagccat
     5581 ggcctggcga aggatataat ccccctcgtt ctcgagggtg ttgatttcca ctaaccactc
     5641 catgaccatg ggatgatctt tcttgtggcg aagaccccgc acaaccttca ggacaaggcc
     5701 gatggcgtta ttgaggatga cggcaagctc gatgatttcc cgggtggggt ttttcacctt
     5761 gtagagatgc atgcggatgg ccgatgcttc gatgaggtcc aggatggaat ccatcttgcg
     5821 ggcaagggca tggatgtctt cccggtccag gggcgtgatg tacgactggt ggagcttgga
     5881 atagatgata tgggtcactc ggtcggcgtc gtgctcgatc tctttgaggg tgggaagttt
     5941 tgcttcggaa tgctcgtagt ggttcagcat atcgaggaag agcaaccctc ccttttcgat
     6001 gagggctccc agttcctcga agtggtcgaa aaatttctcg ttcttgggga tgagtctcat
     6061 ggaaacaagc agcctcctaa acgaaacggg ttgaggaaac gaggcggatc ggtccttctt
     6121 tgctgcttgg agctcccgag aattgcttgt ggctggtgaa taccacgacc acggggaagc
     6181 gtcaaggaaa gggtgcgcga gggtgaggac gacgatcggg cccccggatt ctccggcccg
     6241 gctatgggcg agacccggcg gattccgacc tcaccgggtt tctcagggtg ccgatccctt
     6301 ggatctccac ctccaccacg tctccgtgct cgatggggcc gaccccgctg ggagtacccg
     6361 tggcgatgac gtccccgggc atgagggtga aattcttgga cacaaaggcg aggatttcgg
     6421 gaatgggaaa aatcatgtac cgggtattcg agtcctggac cactcgtccg ttgagtcgac
     6481 actggagccc cagttcctgg gggtcgccca ggtcctcggg aaggacgatg cagggaccga
     6541 tgggaccgaa ggtgtccagg gattttcccc gaaaccatcc cgaacggtct gccgtctgga
     6601 ggttgcgctg gctcacgtcg ttcatgcacg tgtagccgaa gacgacccct cgggcgtccc
     6661 ggggatcgag gtttttgcac cgttttccga tgacgatggc caactcggcc tcataatccg
     6721 tgcgaagctt ctcgaagccg tacccctcga gaaatgccgg aagcacgatg ggttcctcgg
     6781 ggccgatgag cacgttgggg gtcttgggga agaggatggg ctcggagggg atctcgtccg
     6841 taaatccgcg aaccttcacc gagccgcttt cggcaatgtg gtcccggtaa ttgagaccca
     6901 gggcaatgat cttgctgggg ttcagggtgt aggtgcggtc ggtgtgggca atgggcaacg
     6961 ttaccacggg gtccttcctc ctcgattcca agggcggccg gtcatcccgg tcaatggggt
     7021 tcccggatga ccagcccttc cagtcgttcc ttgcgattga agtccgcggc aaaccgcagg
     7081 gcctcctccc tgctctgaaa agaccccacg aggatccggt tccatcggcc gctctcctgg
     7141 agatccaggg gctcgagacg ggaaggatac cccttggatt tcaactgttc caccagcttc
     7201 tgggcgtttt cggcatcccg aagggaagca atgaggagag tgaagtgtcc cgcagcgatt
     7261 tccgggggcg gagcttcttt gaccgcctcc ttggcggcct ctttggtcgc ttccttggga
     7321 gtctccttgg ccgtcttggg aggcgcggcc ttgtccttac cccctgcctt cgaggcgtcc
     7381 ggggttgcgg gggtcttctc ggccggggac gtcttggagg aattcctggg ttggggagcg
     7441 gccggtgccg gttggtcggt ttccggggcc gggggtgatt ctttctgggc caggtcctcg
     7501 taatagctca atgtctcgag catcttcttg ggactgtccc aggtttccgc cgcatcgggc
     7561 cgctcggggg ccacttcttt gtccaatccg atgaagcgaa ccaggtgggc ccgcagggac
     7621 aggtcttcgg aatcgatcaa gggaagccct cttcccgcca aaacccccag catgaacatc
     7681 caggccagga ccagtaccag ggcgaacaac cccgtgaaca catgcttcgt gctgaactcg
     7741 acacggtgcc gcttgggggc gcgatcgggc tcggccgggt ccggaacgtt cctgcccttg
     7801 gatcgactca tggcctggtc tcctggtcag ccggtctaca ttttttcggg cgccgcaacc
     7861 cccaacactc ccaacgcgtt tccgatgacc cggcgcaatg ccgacgccag gtacagcctt
     7921 gcctgggtca attccggatc gtctcccagg atgcggttcc ggttgtagta gctgtgaaac
     7981 agggagacga gctcgtggag gtagtagggg atataatgag gttccagaga tttgacgcag
     8041 ttgagcagca cctggggata ttcccccagc tgctggagga tctcgagctc ctggggcacg
     8101 gtaagccggt gcaacggggg ttcctcgccg ccctcggccc accggcacga aatcccccgt
     8161 tgcccggcca cctcgaaaag gctcgagagc cgtgcgtggg cgtactgaac atagaaaacg
     8221 gggttgtcgt tggtctgcat cttggccacg tccaggtcga aatccagggg actgtccgtg
     8281 cgccgcgtca gaaaaatgaa ccgggcggca tctttgccca cctcgtcgat gacctcgcga
     8341 agcgtcacga actcccccgc ccgggtggac atggcaacgg gcttgccccc ccggaggaga
     8401 ttgaccagtt gaaccaggat gattctcaag tcggcccggt cccgccccag ggcctgaacg
     8461 cccgccgaca tgcggtccac gtacccgtgg tggtccgctc cccagatgtc caccaccatg
     8521 tgaaatccgc gttcgaactt gttcttgtga taagccaggt ccgcagcgaa gtaagtgctg
     8581 atcccgttgg ctcgaacgac cacccggtcc ttttcgtccc cgaaggcggt gctgcgaaac
     8641 cacttggccc cttccgcctc gtacacgaag cccagcttct ccaacgcttc gatggttccc
     8701 aggatttccc cccggtcgtg gagggtctgc tcgctgaacc attcctggaa ggtgaccccg
     8761 aagcttgcca ggtcctgccg gattccctcg aggatccggt ccccggcaaa ccgggcaaag
     8821 acgggaagca cctcgtccat ggcggaatcc cggtactttt ccccttcctt ttccaggaag
     8881 tcccgggcca ggtcgcgcat gtagtccccc tggtagtgcc cctcgggaaa ggcgacctcc
     8941 ttgcccaaaa gttcccggta ccggtaatag aggctgctcc ccagggtgtt catctggttg
     9001 cccgcgtcgt tgatgtagta ttcgcgctgg acctcgtagc cgcacacctc gaggatgttg
     9061 gccagcacgt ccccaatggc cgctccccgt ccgtgaccga tgtggagcgg tcccgtgggg
     9121 ttggcgctga cgaattccac ctggaccttt cggccctttc ccacggtttg agtcccgtag
     9181 gtcttcccct cccggtgaat ctgggaaagg atggggagcc aggcggcggg ctggatgaaa
     9241 aagttgatga accccggccc cgcgatttcc acctttcgga tcattcccgg gtcagactcc
     9301 aaattctcga tgatccggga cgcgatttcc cggggaggtc tcttgagaat gcgactcagg
     9361 gaaagggccg cgttgctggc gtaatgaccg tgctgggaga atttgggaat gtccagttcg
     9421 atgctcaggg aatcgggaag ggtgggggaa tcgaggaccc gagcgacggc ccgggagagc
     9481 gtttcctcga cccgttttcg cactatgccc atggcgatgg tatcctccta tcggttcgta
     9541 aacggagacc ggtttcccgg gtccccggcg ctcactcact tgcgccggac cttcaggccg
     9601 aatccaaccc gttcaagaag accttgcacg cgtgtcggcc catgttgcag tagatttcat
     9661 aactgatggt cccggaaaga tccgcgatct cgtctgccgt gatctgttcg ctcccctgtg
     9721 agcccaggat caccgcctcg tcatcttccc ggacgtcggg aatccccgtg acatccacgg
     9781 tgatcatgtt catggaaacc cggcccgcta ccggggcccg ccgtccccgc accaggaccg
     9841 ccccgcggtt ggagagaagg cgggggtagc cgtcgtcgta tcccacgggc agggtggcca
     9901 ctcgcgtgtt ccgttgggtg acgaaggttc ttccgtaccc gatggggctt cccggaggga
     9961 cgtccttgag ctggaggacc ttgcacttga acgtcatgac ggggcgaagg tccgccgggc
    10021 gaaggatttc ccgggaaggc gaagacccgt aaagcatgat cccgggccga acaagctgga
    10081 aatgggcctc gggaatgtcc agcacccccg cactgttcga gatgtgagca taggtcacct
    10141 cgaggccctt gcgggacagc aggttcagga cgtgacggaa ccggctcacc tggattttgc
    10201 tgtaggcctt gtcggcttcg tcggccacgg ccaggtggga cacgaccccc tcgagctcga
    10261 ggccgggcag ggccaggatc tcctcgagaa acccctgggt ccggtcccac ggaacgccca
    10321 gtcgccccat ccctgtgtcc accttcaagt ggaaaggggc cggaaggtcc cggccgcggg
    10381 cggcccggct caactcccga gcgtggtccg gtcggaacag gacgggacgg acttgcagac
    10441 ggacggcctc ttccatctcc ccgggttcga gacccaggag caccaggatg ggtacacgga
    10501 tccccgcaga ccggagctcc cgggcttccc aaaacttgct caccgcaaga aagtggaccc
    10561 cggacttttc gagctcgcgg gccacgggga ccattccatg gccgtaggca tccgacttga
    10621 cgacccccag gacccgggtg gcgggatgca gccgcctgca cacctgctgg tagttgtgcc
    10681 gcaacgcccc cagatcgatt tctatccaac tcgacggcat tccctcatcc cgcgaacccg
    10741 ttcggccctc ttctttcccc ggcggacccc atgagatctc tttgcaagaa acggcaaata
    10801 tgttaggcaa ttcggcacca aaagaaaagg gaatcttgga aaggccacca tgatgcttaa
    10861 aggctacctg ttgaccgttg cgggtttgat ctgcttcttc gagggactgc cctacctggc
    10921 cacccccgac cagctcaaga ggtggcttct caaggtctgc accctgcctt cctcttacct
    10981 gcgcctcctg ggaggtgctt taatgattct cggcctgctc ctggtttatt ggggccgacg
    11041 cctgggggga tgagccgtga aatcccgaga ttgtctcgaa tcggatccgg cgccgtcgag
    11101 tcccggctac tccctggagg actacgacta ccccctgccg gaatccctca tcgctcagaa
    11161 accctgctca aggagggaat cctcccgcct gctggtcctg gaccgcgccg ggaaaagggt
    11221 gcaacaccgc cggtttgacg atctggcccg gtttctcgac cccagggacg tgctggtggt
    11281 aaacgatacc cgcgtggtcc cggcgcgtct ccatgggacc aaggaaaccg gcgggcacgt
    11341 ggaaatgctg gtcctcgagc ctttcaaaaa gccggagtcg gaagctcggg acgggtaccg
    11401 ctgcctcatc aagtcatcca agcatcctaa agagggaagc gtcatcctcc ttgggaacgg
    11461 agcccgtctc acggtcctct ctcccgtccg ggacggaatg gcccgaatcg ccttccccga
    11521 caccgtgccc ctttcggacc tgctcgagga agtcggagaa gtgcccctgc ctccctacat
    11581 ccgccgagat cggcccgggg attttcccga agacgttgcc gactaccaga ccgtctacgc
    11641 ccaaagaccc ggggccgtag cggcccccac ggcgggcctc cacttctccc tccccctcct
    11701 cggggaactc gaccggctgg gggtgacacg ggtgcccgtc accctccatg tgggttacgg
    11761 gaccttttcc cccatccggg tggaagacat tcgccttcac cggatgcacg cggaatttgc
    11821 cgaaatcccc ccgggttccg cggaaatcat cgaacgggcc cgaagagaag gaaggcgcat
    11881 cgtcggggtc ggaacgacgg tggtgaggat tctcgagtgg gtttgccacc ggttcggggc
    11941 cgtcctcccc ttttccggct attgcgacca ctacatcatt cccgggtacc ggttccacgt
    12001 gatcgacgcc atggtcacga actttcatct tcccaagtcg accctgctgc tgctggtgtc
    12061 ggcttttgcg ggccgggatc gaatcctcga ggcgtaccgg gaagcggtcc gcgcctcgta
    12121 ccggtttttc agctacggcg atgccatgtt gattctctga caggacctat ttctcgtgaa
    12181 ttttgaaatc ctggccatcg acccttcctg ccgagcccgg cgcggtcgac tccaaacccg
    12241 ccacggaacg gtgcagaccc ccgtcttcat gcccgtggga acccagggct ccgtcaaggg
    12301 cgtttccccc gacgaactcg agtccctggg aaccgagatc ctcctgggga acacctacca
    12361 tctctacctg aggccggggg ccgaccggat cgcccgcctg ggggggctcc attccttcat
    12421 gcactggaat cgctcgatct tgaccgacag cggaggattc caggtcttca gcctcgccaa
    12481 aatccgcaag atcgaggagg aaggagtggt ctttcagtcc cacatcgacg ggtcccgcca
    12541 ccgcatcacc cccgaggggg ccgtccggat ccaggagcag ctgggatccg acatcgccat
    12601 gtgcttcgac gaatgcacct cctatcccat cccctacgaa tacgcccgtc aatccatgga
    12661 gctttccgtc cgatgggcgc gacggtgcaa ggacgctcaa acccatgccg accaagccct
    12721 gttcggcatt gttcaaggca gcgtgtttcc ggacctgaga cgggaatgcc tggatcgact
    12781 catggaaatc ggtttccagg gatatgccct gggaagcctt tccgtggggg aacccaagga
    12841 ggcaatgctg gcggtcctcg aagcccttgt cccgggcatg ccctcctacg cccctcgata
    12901 tctcatgggg gtgggaacgc ccgaggacct ggtggaaggc gtgcaccggg gggtggacct
    12961 tttcgactgc gtcatgccca cccgcaacgc ccgaaacggg atgctcttta cctcaagggg
    13021 aaacctccag atcaagaaca gccgatatgc cgaggacccg gaccccgtcg aacccgggtg
    13081 cacctgctat acctgcctgc ggttttcccg ggcctacctg aggcacctgt acctttcgag
    13141 ggagctcctg gcctatcgcc tgaacaccct tcacaaccta cactactacc tgtctttgat
    13201 gcacaaaatg agggaagccg tcgaggcggg gacgttcctg caatggcggc gggacttcta
    13261 tgccgggcgg gaagcctcgg agatttgaat ccgctccctt cccgcccctg gagccgttct
    13321 cgatcggcaa ggtcgacctg ccgggggccg ttgcccgggt atcgctttct gcgggttttc
    13381 taggcagggg cttcggaagg tcggttctct gcggccagca gggccagggc cagggccctt
    13441 tcctcgcgga accttctcaa taccgacccc cggatgtccg ggttcgaacc ggaaaactgg
    13501 gtggaccggg aatcgtagat ggaccgaacc aggtcgcgga gaggcccggg accgccttcg
    13561 gtccggcttt cttcgatggc ccgacagaag atgttggcgg caccccgcgg accgtgctgc
    13621 accgccgtgc tccaaaggac ttccctcaag gcccggggct gcttgccgat attcacgccg
    13681 gtcctctcgt agatttcttc catggccgga aagtagtggc tttcctcgat aaaatcacgc
    13741 tgcaaccggg caaaccggac ggggtcctcc tcggcaatct tttgccactc ggcgggcatg
    13801 ccgccctggc gactgcccgt gtcggccggt ccggccgctt ttaggcgccg ggcccactcg
    13861 ggggcatggt cctccagata gctgagaaac tggcgcatgg ttcccacccg ggaggcgatc
    13921 tggtaactgc cataggacgt gccccccttc tcgtcgtaac cgatggcctt gattcccatt
    13981 tcgccggatt caaaacgggc ggacagttcc cctagccttc cgacttcccg cggcaagggt
    14041 cggccggaaa ggggctggga tgccgaggag gcccccggcg ccgttgccgc cccggctctc
    14101 cgggacgcaa cagcctggct gtagtaggcc gccagctggt ctcttctgcc cgtgaccgca
    14161 gccaggttca gcaaggcgct ggtggcgaga tcgttcatct ccccccctcg aggggcagaa
    14221 accccgccct gtttcattgc gtcaatattt tggccccggt cccgggcgac ctggatgctc
    14281 tgcaggtcgg cctggagcga atccgtcccg cagctcgtgc ccggatcgtc aacggattga
    14341 gcttcggtca ggaccgttcg aaagagcttc tgttgactcg aggagacgat ggaacgatag
    14401 gcccactcga ggttggcggc ctccgccgca cggttggctc ccgggatttc cttgacattc
    14461 acggccatac actctccctt tgcggcatcg tggatgtttc caccggcaat ttagcaagat
    14521 ccatgctcac agaggccgcc ctcggtccgt ccccggggag cgcacggacc ggggacgctc
    14581 cgtttcctcg gagaagaacc ccttcttttc cttccttccg cattcacccc gcccgaaaaa
    14641 aatggtatga agagatcccg taatggttgc tgcacgtgag aacacaggca cttttttccc
    14701 tccggcgctg cccaaccctg catctttttc cttcgaacga tttcttcatg cccttccccc
    14761 cggcaacgac agtcttgaaa aagaccgggg agaagatctt cctttctgcg caggcgaagt
    14821 ggagagcggg ttttcttccc ttgttcgagc tgctgccttt cttcgtaatc ccttggcccc
    14881 tttttccccg ggggatctcc gggagcgccg tccacgggag acctccgagg aagcgagaat
    14941 cggtcatgcc cggcaagttt gaaatcgatc gttccccgaa cggcggctgc tgcgtctgtt
    15001 ccatgaacga gcgggagctt tacctggtgc gggaggcatc gagcggcagg ctggtggagg
    15061 tctgttcctg ctgcttgctg gaaagggtcg atgattttgt cctcgacaac acccggccat
    15121 ggcccggcct ggaacgatcc tgaacgcccc cgcgttccac ggctgatcga aatcacaacg
    15181 gatgcccgac accgtccctt cccgccctcg actttcgaaa catccgattg aagagtcccg
    15241 tccctgaaaa tccaaccaac ggcgctcttg gcgggcgggg acggggcccc gcccctgcat
    15301 gcaggacggt ctgccggtag gctcgggggc gggctttctg cacacccgtg aaccgggaaa
    15361 agatcggtca tcttgggttt gagtgttgac cttttaggcc gggagcatat aaatatacac
    15421 tgatatgtta ctgagtttgt gcgcctggat ctgcgtagag tgaaacgatt ccaagagcac
    15481 tcgggcgtca tgcccgggac ggcgggtact cggcggatat gctcgccctt tcccggcttc
    15541 cccctgcgtg ggaatcgtcc gtgccacggg ttgcgccccg tttggtggaa gtttctcctt
    15601 ccgcaggaag gacgaaacgg gattcggacc ggccgaatcg ctcgatcccg gtgccgctcg
    15661 gatcgtgatt gcggccgccg acggctgcac ggcgatcttg cagccgctca tttcattgcg
    15721 ctgaagaagg cgagcgttgt ctgcccggaa gccgccgggc ccgtctctgc cggttcggca
    15781 tggaaacagg acggatgaat gggaatgatc gcccgaggaa ggccgttaaa gggcctcgat
    15841 atggcggaag agaacgagga acatctcgcg gaaaaggagg caatggtgct gcaagacgag
    15901 cactatcaga cagtggcgtt tctcgcgaag agtctttcgg acgaaaaccg cctgcggatc
    15961 atcctttgcg tgagcaacgg caagaagtcc gtctcgagca ttgtcgagga actcggcatc
    16021 tctcagcccc ttgtttccca ccacttgaag gagctcaagc gctcgcttct cgtcaagatt
    16081 gagcgcagcg gccctttcat ctactacgag ctcgttgacc cgagaatcct ggatgtcatt
    16141 cgcactttga gcaccgtagc gacagacctg ctgaccacaa ggaagacctt ttaggccggg
    16201 gggaggtgac cggaatccat atggaaaaga tccggaagat cgttggccga atggaccctg
    16261 aaaaggcttt ggaagagatt tccgaggtct tgaaggtcct cttctccgcg ttgggcgaag
    16321 aggcgcgctc gcaattcctc tgggacctcc tgggggaatc gagtggggat aaggtgtcga
    16381 gcctggtcca cctgtgactg gccgaatgca tgggcgaagg tgtcgacccg acgcagatgt
    16441 gccagaggct ggtggacaag gttgctcaat caaagcagct catgaccatg acggatccgg
    16501 atcttctggt gctcttcgag gactggtttg aagaactgga agaggagatc gtgtcgctgg
    16561 tgagggaacg tggtcccctg gaccccggtg agcttgccga aaggaccggg ctttcgctgc
    16621 gaggggcaac gtttctcctc tcaaaactca agcgtgaagg aaagctctaa cacccgattc
    16681 atgcccggca ggaaaggttt ccatgcagaa ccggaagacg acggtcgtag ccctggcggc
    16741 gatactgctc acggtcgggg ccctgtggtt cgccaaccgc g
//
LOCUS       DAOOVC010000082        16775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2329_length_16775_cov_6.490730, whole genome shotgun sequence.
ACCESSION   DAOOVC010000082 DAOOVC010000000
VERSION     DAOOVC010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16775
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2329_length_16775_cov_6.490730"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PLM79_11130"
     CDS             complement(<1..217)
                     /locus_tag="PLM79_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86908.1"
                     /translation="MIKRITVENYMAHKRSTIELAEGVTVITGPNNSGKSALVEAVRS
                     VAQNPPSGRHAVRHGARSAVVRIELDTG"
     gene            495..1637
                     /locus_tag="PLM79_11135"
     CDS             495..1637
                     /locus_tag="PLM79_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOV86909.1"
                     /translation="MSKLFESSRIGTLILDNRFVRSATWEGMAGEDGAATPPLTDLMV
                     RLARGKVGLIITGHAYVRREGQAGPRQLGVYGDELLAGLRQMASAVHEAQGKIVLQIA
                     HAGVHAASNLTGLEPLGPSVLDHPQAPRCRSMSGEEIREVAAAFGRAAERARTCGFDG
                     VQVHAAHGYLLSQFLSGFYNRRTDSYGGSLENRARFLCHVVSEIRRVSGRDFPLLVKI
                     NSEDFVPGGLTVEEMIRVARRLEELGVDGIELSGGTILSGKYNAVRRGDVTLEGEGYY
                     RQAAKRCKESLGIPVLLVGGIRSFPVAERFVEEGYADFISLSRPFIREPNLVARWSSG
                     DIRRSECLSDNLCFKPAMRGPGVYCLTEEIRNRTASGPKKTTGTMG"
     gene            2177..3118
                     /locus_tag="PLM79_11140"
     CDS             2177..3118
                     /locus_tag="PLM79_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV86910.1"
                     /translation="MNPEKMKVLLLVDGSYQAYEAVNYAGSVLPASRCEVTLFHVMSK
                     VPDAFWDLENDPVWHQKILTVRGWEKQQEHKIYDFMERSRQLLRKMGFGDDAVRVEIR
                     ERKEGIARDIVHEAGRGYDAVMMGRKGHGSLDESVLGSVASKLMGKLGERAVWVIGGK
                     PGTEKILIAMDPSEGAMRALEHAVRTLNGSAREICLVHVVRGVAGSDKGLEEFFPEEY
                     RRGLLEEAKEAVRGTFEKARERLVASGIPDPQISTRVITGVTSRAGAVMNLAREGDFG
                     TIVVGRRGISKVEAFDMGRVSNKLIQMAKDRALCVVG"
     gene            3328..3822
                     /gene="nuoE"
                     /locus_tag="PLM79_11145"
     CDS             3328..3822
                     /gene="nuoE"
                     /locus_tag="PLM79_11145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOV86911.1"
                     /translation="MVPSRLRDELRESVVRAEHPREKAVDVMFALQDHYGYMNDRAMA
                     EAAEILGMTPLELEELATFYDFIYREPVGKYVIRVCDSAVCWMNGLESVLDHLQSRLG
                     ISTGETTPDGLFTLLPVCCIGYCDRSPAMLVNRKVYGNLTGERIDEILEDLRRQAALS
                     QRND"
     gene            3815..5107
                     /locus_tag="PLM79_11150"
     CDS             3815..5107
                     /locus_tag="PLM79_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV86912.1"
                     /translation="MIDGMYPRVLFQNRKPDRIATLDEYRQNGGYRALERVLKEHSPR
                     EVCRIIEASDLRGRGGAGFPAGKKWLAVPEDGAYPRYLAANADEMEPGTFKDRVLIHA
                     DPHLILEGMTLAGYSFGASKAFLFVRPSYEGIVRIMEREIGIAQRAGYLGKNILGTDF
                     SFEIVVHRSGGRYICGEATALLNAIMGKRPNPTKPPPYPTVKGLWERPTVVNNVETLA
                     NVPHILRNGPEWFKGLGLKEKTAGTKLFCVSGKVNRPGCYELPMGVPLSEIIEEHAGG
                     LQPGSELKAVLPGGASTRFLTPDHYHVPMDFDDLGAIGHRLGTAAIMVFDRNTCLVGA
                     TLNLTRFFARESCGWCTPCREGLPYLRELLDRIENGEGKEEYLPLIRTMCAQLWNSYC
                     ALAPGAASPIESLLSFFEDEVREHIVQKRCPFPSVSGS"
     gene            5306..7759
                     /gene="nuoG"
                     /locus_tag="PLM79_11155"
     CDS             5306..7759
                     /gene="nuoG"
                     /locus_tag="PLM79_11155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOV86913.1"
                     /translation="MPKLIIDGLEIEVPSGTKVIEAAERLGIVIPRFCYHEALGSVGA
                     CRMCAVKFLQGPFKGVQMSCMIDAQDGMVVSTQDEEAVLFRKRVIEWLMLNHPHDCPV
                     CDEGGQCLLQDETVSGGHGVRRYPGRKRTYRDQFLGVFLAHEMNRCIHCYRCARFYQE
                     FAGGRDLGTLCLGDRVYFGRFEDGQLESPFSGNLVDLCPTGVYTDRTARYRARRWDLQ
                     RAPSLCLHCSLGCNTIANARYRSVVRIEARYNARVNGHFICDRGRFGFSWANGEPGEL
                     ERPREARAGGEPVSLDHAVVKITEALGAILDSAGPSAVACLGSGRSSLEVLESLKRFC
                     AALGISGPDLFLTSSAARKVRRAVSLLKPHLACSLREIEEADSILVIGCDPLGEAPML
                     AYSLRQANRKGAPVTVLDPRPVELPFSFELISADPRDLNACLRFLSSGGSGEVPSEIR
                     TSLERVVRELDGRHRPVIVCGTEMVEETAPELAAGLAEFLGGQADGARLFYVLPGPNA
                     YAAGLFSETETGSFEETLGSIEKGIVKALVVVETDPMGFHPDRERVQRALDALDLLVV
                     LDYLPSLTVDRADVFLPTSTVFEAGGHFVNQEGRVQFAHPTHRGGIPIRGEHPPRIFR
                     DFVPGETRPAGEILGMLLERMGPPGGPDSGTPDRSPVRNAPAPVRDLPGLFSDLPPEG
                     QRIFSAGDPAGSPAKVESRGSAPPLDGNFLLLPTDSTFGTEELSSYSPAIRSVAGGPA
                     VFVHRADADRMGWREGDAVALDLAGGLIKVRVAPRDRMARRVLVLPRHWSLDWRKIRG
                     GPLLIPESRIRKIRESAPL"
     gene            7806..8807
                     /locus_tag="PLM79_11160"
     CDS             7806..8807
                     /locus_tag="PLM79_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOV86914.1"
                     /translation="MNANEWTPGQILDTSGFYWKTCTLHCGVKLGVFSVLGEERLGSR
                     EVARRLGADPRATAMLLDALAAMKLLVKSRDGYANTSSAQTFLRKDSPQYLGHILMHH
                     HHLVNSWFQLDAAVRTGKSVSGSSSSTRDEEWREAFLMGMFNLAMNIAPRIAEEVDLS
                     GRRHLLDLGGGPGTYAIQFCKKNPALRATIFDLPTTRPFAQKTVARFGVEDRVDFQEG
                     DYLEGEITGRFDVAWLSQILHGEGPEGCRSILRKAVAALEPGGLILVHEFILDNTRDR
                     PLFPALFSLNMLLQTAEGQAYSDGEIREMLSEAGVTDIRRLPFEGPNQSGILAGVIP"
     gene            complement(8868..9443)
                     /locus_tag="PLM79_11165"
     CDS             complement(8868..9443)
                     /locus_tag="PLM79_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV86915.1"
                     /translation="MKAVAFNGSARKDGNTAVLIKTVFEELEKEGIETELVQLAGRKI
                     RGCTACFKCFTNKDQRCSVKEDYANECIEKMLAADAVILGSPTYFADVTAELKALIDR
                     AGLVAIANDSMLRRKVGAAVVAVRRGGAVHAFDTINHFFLINQMIVPGSIYWNMGVGL
                     EKGEVAKDEEGLRTMKVLGENMAWLLKAIRG"
     gene            complement(9522..10508)
                     /locus_tag="PLM79_11170"
     CDS             complement(9522..10508)
                     /locus_tag="PLM79_11170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOV86916.1"
                     /translation="MKVFVTLHLPRELLTEVEKTHEVEMNLEDRPLDRGELLDAVGDK
                     DGLLCSIHDRIDEELLERASALKVIANFGVGYDHVDLEAATRRGIPVGYTPGVLTDAT
                     ADIAFALILAVARRVVEGDELVRSGRFPVWAPFNFLGRQVAGKTLGIIGMGRIGKAVA
                     RRAAGFDMRVLYHNPNPLLPEEETEYGSQWVSLETLLKESDFVSLHVPLTPRTRHLID
                     RDQLALMKPSAFLINTSRGPVVKESALVEALRAGNILGAGLDVYENEPALAPGLADFP
                     NVVLLPHVGSATYETRTDMGRMAVENLLAGLAGQRPPNCLNWERLPGGKGKK"
     gene            complement(10637..11695)
                     /locus_tag="PLM79_11175"
     CDS             complement(10637..11695)
                     /locus_tag="PLM79_11175"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOV86917.1"
                     /translation="MTTTAEIQKILAEGVARGKRAAVATVVERSGPGPRGPGSQLVLW
                     ADGTMMGSVGGGILEGVIEERGRQVLDNGRPAWVSLDLGSGAEEDCGMVCGGKVRVLV
                     DFIDGDRPRVRSFYGEILSELQARRQFHVVTSVRGTEELFTNQFLLREESVASSRSDI
                     SEELPRDLLRNVRKIAWRLLDFESVRYFVHPHGFLGDVYIFGCGHVAQQLAPLCRLVG
                     FRVVVLDDREEFANRERFPSADEIRVLESFEGVTDLLPIDPECYLVIVTRGHRSDGLV
                     LSQCLRTHAGYIGMMGSRKKRDAAYAALTREGFTEQDLQRVFCPIGLAIGAETPEEIA
                     VSIVAQLIEVRANRLRRL"
     gene            12085..12714
                     /locus_tag="PLM79_11180"
     CDS             12085..12714
                     /locus_tag="PLM79_11180"
                     /inference="COORDINATES: protein motif:HMM:NF016811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HOV86918.1"
                     /translation="MKKSLGIPLTVLFGLACFVAGARFSGIPASEHCRLPHSRVLYYV
                     DPLNPAHKSDQPGLTPYGLKLDPVYENARFSRNGPSPLASTGIGGGSGKPETMAVPTE
                     PVSRAKETSSPRVPGWSGTRKPDTRADAEVQREIDPVCKMPVLPDRAARAGLAADYRD
                     KTHYFCSRWCKERFQKMPQQVLSRPAEKPIPLAQSAMPGPVNREDPGSH"
     gene            complement(12708..15320)
                     /locus_tag="PLM79_11185"
     CDS             complement(12708..15320)
                     /locus_tag="PLM79_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV86919.1"
                     /translation="MLRFLDFFRKGKALEGESPEQAAFRKKYQHFENLLAANNKALEL
                     MTELEQTCYGPKPFSLEYVIGQTESLLSYVNRIADELNALSNNKFVRLFDAVERIGVS
                     VLQELVRKRTVEAGRFTLPMQDLSLERLAEVGGKAASLGEVYNRVHLPVPRGFAITAY
                     ACSHFMHFNRLYEESERVLKGLDVEDTEKLLECSRKIQSLILDAPLPPDLENAVLNEV
                     DSLIRELGPDIRMAVRSSATCEDSEASFAGQHSSVLGVGRSNFLQAYKEVVASTFNPR
                     AIYYRRSKGYPDEYVIMSVLCLVMVNAKSSGVLYTRDPNDPRRDVILVNGVWGLGVSA
                     VEGSAATDFFVVDKARRQILSTWTAPKETMLVLADPEGLEERKVPPELRTRPSLESDQ
                     LLSLAEAGILLEEHYGQPLDIEWALDSSNRIVLLQSRPLNVDLKTVRGDVPTLGSDLE
                     SLRRKFPDNPILLHQGVTASRGKASGIAYVLTSDHNLLNIPVGSILIAKQTSPRYVSI
                     LGRVQAIVTDVGSITGHMASVAREFGVPTLVGTGNATEVISHGEEITVDATNCLIFQG
                     RVESILERKKAVNPMKGSPTYKACHAALKKMAVLNLTDPENENFSPDGCRTVHDVVRF
                     AHEMSMREMFRISDRVEMDKRYAVRIKAPLPMKILAVDLQGGLNIPPGASEATMEQVT
                     SIPFQALLRGMTHPDVRWVGAVGVDWRGFATIMAESVFRDPTMDDRMGGPNYVVLSNE
                     YFNFNSRLGYHFAVIDAYCGPQVNDNYVAFSFKGGAADIGRRSRRALLIAKILKRLGL
                     KTTLKGDMVRGEIKKYDCSELLTRLDMLGRLIGAVRLLDMVLSDDGRIDWYVDEFFNG
                     NYSFQRKAQGTSGQ"
     gene            complement(15525..16775)
                     /locus_tag="PLM79_11190"
     CDS             complement(15525..16775)
                     /locus_tag="PLM79_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOV86920.1"
                     /translation="MKGTRGDQRDRGTIGKPRTSGARKTQGSRGKLMQMFQGKPIYLD
                     YNATTPIDPRVAEAMLPYLLEHFGNPSSSHVYGRKAKEAVDKARTQVAEMLGCREEEI
                     VFTSGGSESNNYAIKGIARALRSRGRHLVASTIEHPAVTEVCRHLEEEGFRTTLVPVD
                     GNGRVDPRDVEAAITAETILISIMHANNEVGTIQPVEEISRIAHSRGILVHSDAAQSV
                     GKIPVRVHELGVDLLSVAGHKLYAPKGIGALFVRSGVQPDKLLDGANHEMNRRAGTEN
                     VAQIVGLGEACAMVRENQSSYEAHLRRMRDRLEEGLAARLSEVRVHGHPRERIPNTSS
                     LGFRGLSATQILSELRTVAASAGAACHSDHVCVSGVLQAMQVPLEYAVGTIRFSVGRF
                     TSADEVDTAVREIAAVVARLRQGR"
BASE COUNT         3036 a         5111 c         5289 g         3339 t
ORIGIN      
        1 cccccgtatc cagctcgatc cgaaccacgg cgctcctggc gccgtgacgg acggcgtgcc
       61 taccggaggg cgggttctgg gcgacggatc ggacggcttc cactagggcg ctcttcccgg
      121 aattgttggg tccggtgatg accgtgactc cttcagcgag ctcgatggtc gagcgtttgt
      181 gggccatgta gttttcgacg gtaattcgtt tgatcatcgt tcctgcggtc gaatgcctcc
      241 ttgccggaat tgaggaacct gctatcggaa ccctgggctt gagcgccgaa ttctaccgga
      301 tccacggatt ctgaccagag ctatttgact ccccaaaccg gagtcatgcc ggcccgggag
      361 ggctgtcgga gagggaaggt ttgaattgtc tccaaagccc gcaacgaata gaatgtcccg
      421 ctacggcgca gtgggcgccc aggaccggtt tccccggtgc cggatgttcg ttggaggtgt
      481 tttgggagtg gaacatgtcg aagctttttg aatcgtccag gatcggcacc ctaatccttg
      541 acaatcgttt tgttcgatcg gcgacctggg aaggcatggc cggggaggac ggagccgcga
      601 cgccccccct gacggacctg atggtgcgtc ttgcccgggg caaggtgggg ctgatcatca
      661 ccgggcacgc ctatgtgcgg cgggaggggc aggcggggcc tcgccagttg ggggtctacg
      721 gggatgagct ccttgccggt ttgcggcaga tggcttccgc ggtgcacgag gcccaaggca
      781 agatcgtgct gcagattgcc catgcggggg ttcatgccgc ctcaaacctg acgggcctcg
      841 aacctctggg gccctctgtt ctcgatcatc cccaggcccc ccggtgtcgt tccatgtccg
      901 gggaggagat tcgggaagtg gcggcggctt tcggccgagc tgccgagcgg gcccgcacct
      961 gcggctttga cggcgttcag gttcacgcag cccacgggta tctcttgagc cagttcctgt
     1021 ccgggttcta taatcggcgc acggactcgt acgggggaag cctcgagaac cgggcgaggt
     1081 ttctctgcca cgtggtgagc gaaattcggc gggtctcggg gagggatttc cccctcctgg
     1141 tgaagatcaa ctccgaagat ttcgttccgg gaggcctcac ggtcgaagaa atgatccggg
     1201 tggcgcgccg actcgaagaa ttgggcgtgg acggcatcga gctcagtgga gggaccatcc
     1261 tctccgggaa gtacaacgcc gtgagaagag gagacgtgac ccttgaaggg gaggggtatt
     1321 atcgacaggc ggcaaaacga tgcaaagaat ccctcgggat tcccgttctc cttgtggggg
     1381 gaattcgttc ctttcccgtg gccgaaaggt tcgtggaaga aggctatgcc gacttcatct
     1441 ccctgagcag accgttcatt cgcgagccaa acctcgttgc ccgctggtct tccggggata
     1501 tccgaagaag tgagtgtctc tcggacaacc tttgcttcaa acccgccatg cggggtcccg
     1561 gcgtctattg cctcacggaa gagatcagga acaggacggc ttccgggcct aaaaaaacca
     1621 ccgggacgat gggctgatcc cttggagtcc gggaggttcc ctttccgcgg gaggggaaat
     1681 cctctccggg aaaaggtctt ccggagcact catcactgat cggcgaatgg gtctcttccc
     1741 cctgaagacc gtacctgtgg atcctattgt aagaaatgct gaaaggatgc gggagaaccg
     1801 tccgtgagaa gcaacccggg tggcttcccg ttccggaatc tggtaggatg aatgccgggg
     1861 cccggaaccg caggggaaat gggttgttac cggtttacag aggatcgtcg ttccggcgga
     1921 cctcggattc gacgcggcag gcgccgaaat gcaggaagga gggccggttt tcgcccacgc
     1981 agcatggatc ggccccttcg ttgaactggg cctgcggcag gcaattccgt gcaggggcgc
     2041 ttcccggttc cgaaatccgc cctcggtttc ggccccgtcc agcggcaccg gcacgggcct
     2101 ccggcaggag gtccggctag gacccggccg gaggccgagc aagaggtgaa ccaaggcgag
     2161 cgtgaaaggg gactgcatga atcctgaaaa gatgaaggtg ttgctcctgg tcgacggatc
     2221 ctaccaggca tatgaggcgg tcaattatgc cggcagtgtg ctgcctgcgt cccggtgcga
     2281 agtcaccctc tttcacgtga tgagcaaggt ccccgacgcc ttctgggacc tggaaaacga
     2341 tcccgtttgg caccagaaga tcctgacggt tcgtggctgg gagaaacagc aggagcacaa
     2401 gatctacgac ttcatggagc gttctcggca gctcctgagg aagatggggt tcggtgacga
     2461 tgcggtcagg gtggaaatcc gtgagaggaa agagggcatt gcccgggaca tcgttcatga
     2521 agccggtcgg gggtacgatg ccgtcatgat ggggcgcaag ggacacggtt ccctcgatga
     2581 atccgtgctg ggcagcgttg ccagcaaact catgggaaag ctgggcgaac gggcggtttg
     2641 ggtcatcgga ggaaaacccg gcaccgagaa aatcctgatt gccatggatc cttccgaagg
     2701 tgccatgcgc gccctcgagc atgcagtccg cactctaaac gggtccgccc gggagatctg
     2761 cctggtgcac gtggttcgag gggtggccgg ttcggacaag ggtctcgagg aattcttccc
     2821 cgaagagtac cggagggggc tcctcgagga ggccaaggaa gccgttcggg gcaccttcga
     2881 aaaagctcgt gaacgcctgg tggcctcggg aattccggac ccgcagattt ccaccagggt
     2941 catcacgggg gtgaccagcc gggcgggtgc cgtcatgaat cttgctcgtg agggcgactt
     3001 cgggaccatt gtggtgggtc gcaggggaat cagtaaggtg gaagctttcg acatggggcg
     3061 ggtcagcaac aagctcatcc agatggccaa ggacagggcc ttgtgcgtgg tcggatgacc
     3121 aagaaccgat ccatgaacac gggttgatcc aaaacaggtc gttttccctc tcgcggcctc
     3181 tcccatccgg ggagggcggg aaattccctc tcccggcttc ccacccctat tccttgactt
     3241 ttcccggctc acgtttagta ttttcctacc gtggcgcatc ttggaacgtg gcgtattttg
     3301 ggaccgggat ggaagcaggg tgggtgcatg gtgccgagcc gtcttaggga tgaattgcgg
     3361 gaaagtgtgg ttcgggcgga acatccgcgg gagaaggcgg tggatgtcat gtttgccctg
     3421 caggaccatt acgggtacat gaacgaccgg gccatggcgg aggcggccga aatcctgggc
     3481 atgactcccc tcgagctgga agagttggcc accttttatg atttcattta ccgggagccc
     3541 gtaggaaaat acgtcattcg tgtttgcgac agcgccgtct gttggatgaa cggactcgag
     3601 agcgttctgg atcatcttca atcccggttg ggcatttcca cgggagaaac cacccccgac
     3661 ggcctgttca ctctccttcc cgtctgctgt atcgggtact gcgatcgatc ccccgccatg
     3721 ctggtgaacc gaaaggttta cggcaacctc accggggaac gaatcgacga aatcctcgag
     3781 gatcttcgcc gacaggccgc cctgagccaa aggaatgatt gacggcatgt acccccgggt
     3841 gcttttccaa aatcgaaaac ccgaccgcat cgccaccctg gacgaatacc ggcagaatgg
     3901 gggatatcgg gcgctggaac gggtgctcaa agagcattcg cccagagagg tctgcaggat
     3961 catcgaggcg tcggacctga ggggccgggg aggagcgggg tttcccgccg ggaaaaagtg
     4021 gctggccgtc cccgaagacg gagcctatcc ccggtacctg gccgccaatg ccgacgagat
     4081 ggagccgggg accttcaagg atcgagtgct catccatgcg gacccgcacc tgatcctcga
     4141 ggggatgacc cttgccgggt actctttcgg cgcgtccaag gcgttcctct tcgtgcgtcc
     4201 ctcctacgaa ggcatcgtcc gtatcatgga gcgggagatc gggattgccc aacgggcggg
     4261 ctacctggga aagaacattc tcgggaccga cttttccttc gagatcgtgg ttcaccggag
     4321 cggcggacga tacatctgcg gcgaggcgac ggccctgctg aacgccatca tgggcaagcg
     4381 gcccaatccc acaaagcctc ctccctaccc gacggtcaaa ggcctttggg aacgccccac
     4441 ggtggtgaac aacgtggaga ccctggctaa cgtgccccac attctccgaa acgggcccga
     4501 atggtttaag ggcttgggcc tgaaggaaaa gaccgcggga acgaagcttt tttgcgtcag
     4561 cggaaaggtg aaccgccccg gttgctacga acttcccatg ggggtcccgt tgagcgagat
     4621 catcgaagag catgcggggg gcctgcaacc gggttccgag ctcaaggccg tgcttccagg
     4681 cggggcctcc acccgcttcc tcacgcccga tcattaccac gttcccatgg atttcgacga
     4741 cctgggcgcc atcggccaca ggctgggcac ggcggccatc atggtgttcg atcgcaatac
     4801 ctgcctggtg ggagccacct tgaacctgac ccggttcttt gcccgcgaat cctgcggctg
     4861 gtgtaccccc tgccgcgaag gcctccccta cctgagggag cttctcgatc gcatcgagaa
     4921 cggtgagggg aaagaagagt acctgcccct cattcggacc atgtgcgccc aactatggaa
     4981 ctcctactgc gcattggccc cgggagccgc ctctcccatc gaaagccttc tgagtttttt
     5041 cgaggacgag gtgcgggagc acatcgttca aaagaggtgt cccttcccgt cggtttcggg
     5101 gtcgtagttg ggtgccggcg ctcttcccat cgggggccgg ctcccgtgaa cccatgggtc
     5161 ttggttttgc cccggcgccc caaggggggc gatagggacg attgcgccgg ggaaacgagt
     5221 tggatgtccc ggtcatcccg ggtccgggga catccccgac cccgccggaa ccgacgatcc
     5281 gtggtcgccg ggagaaggtc atcccatgcc gaaactgatc attgacgggc tggaaatcga
     5341 ggtcccctcg ggaacgaagg tcatcgaggc ggccgagcgc ctgggcatcg tcattccccg
     5401 cttttgctat cacgaagccc tggggtccgt gggcgcatgc cgcatgtgcg cggtcaagtt
     5461 cctccagggg ccgttcaagg gcgttcaaat gagctgcatg atcgacgccc aggacggcat
     5521 ggtggtctcc acccaggacg aagaagccgt gctttttcgc aaacgggtga tcgaatggct
     5581 catgctgaat catccccacg actgccccgt gtgcgacgag ggaggccagt gcctcctcca
     5641 ggacgaaacg gtctcgggag gtcacggcgt tcggcgctac ccgggcagga aacgaaccta
     5701 ccgggaccag ttcctgggag tcttcctggc ccatgagatg aatcgatgca tccactgtta
     5761 ccggtgtgcc cgcttctacc aggagtttgc ggggggccgg gacctgggga ccctgtgcct
     5821 gggagaccgc gtttatttcg ggcgcttcga ggacgggcag ctcgagagcc ctttttccgg
     5881 gaacctggtg gatctttgtc ccaccggcgt ttacaccgac cgaacggccc gctaccgggc
     5941 caggcgatgg gacctccaac gggcaccgtc cctctgtctc cattgctccc tggggtgcaa
     6001 caccattgcc aacgcccgct atcgctccgt ggtgcgcatc gaggctcgct acaacgctcg
     6061 ggtgaacggc cactttatct gcgaccgggg ccggttcggg ttttcctggg ccaacgggga
     6121 accgggggag ctggaacggc cccgtgaggc ccgggcgggg ggggaacccg tgtccctgga
     6181 tcatgccgta gtgaagatca cggaggcatt gggggcgatt ctcgattctg ccggaccttc
     6241 cgccgttgcc tgcctgggat ccgggcgatc cagcctcgag gtcctggaga gcttgaaacg
     6301 cttttgtgcc gccctgggga tttcgggacc ggacctgttt ctcacttctt ccgcggcaag
     6361 gaaggtaagg cgggccgttt ccctcctgaa accccacctg gcttgttctc tcagagagat
     6421 cgaagaggcg gattccatcc tggtgatcgg ctgcgatccc ttgggggaag cgcccatgct
     6481 ggcgtattcc ctgcggcagg cgaaccggaa gggggccccc gtgacggtgc tggatccccg
     6541 gcccgtcgag cttcctttct ccttcgagct catttcggcg gatccccggg acctaaatgc
     6601 ctgtctgcgt ttcttgtcct cgggggggag cggggaggtt ccgtccgaga tccggacctc
     6661 cctggaaagg gttgttcggg agctagacgg ccgtcacagg cccgtgatcg tctgcgggac
     6721 ggaaatggtg gaggaaaccg ccccggagct tgcagccggg cttgcggaat tcctgggcgg
     6781 gcaagcggac ggggcgcgtc ttttctacgt gcttcccggc ccaaacgcct acgcggccgg
     6841 attgttttcg gagacggaaa ccgggtcttt cgaggagacc ctgggctcca ttgaaaaagg
     6901 cattgtgaag gccctggtgg tggtggaaac cgaccctatg ggattccacc cggaccggga
     6961 gcgggtccaa agggctctgg atgcgctcga tctcctggtg gtcctggatt atctcccttc
     7021 cctgacggtg gaccgggcgg acgtttttct cccgacttcc accgttttcg aggccggggg
     7081 gcattttgtg aaccaggagg gaagagttca gtttgcccac ccgacccacc gagggggcat
     7141 tcccattcgc ggggaacacc cgccccggat cttccgggat tttgttccgg gggaaacccg
     7201 acccgccggg gagatcctgg ggatgctgct cgaaagaatg ggcccgcccg gggggcctga
     7261 ttccgggacc ccggatcgat ccccggtccg gaacgccccg gccccggtac gcgatctgcc
     7321 gggccttttt tcggatcttc ccccggaagg gcagcggatc ttttccgctg gggatcccgc
     7381 agggtccccg gcgaaggtcg aaagccgggg gtccgccccg cccctcgacg ggaatttcct
     7441 gctgcttccg accgattcga ccttcgggac ggaagaactt tcttcctact ccccggccat
     7501 ccggtccgta gccggaggac ccgccgtctt cgttcatagg gcggatgcgg accggatggg
     7561 atggcgggaa ggggacgccg ttgccctgga cctggcgggg ggactcatca aggttcgcgt
     7621 cgcccccagg gaccgcatgg ctcgccgggt cctggtcctg ccccgccatt ggagcctcga
     7681 ttggcggaaa atccgggggg ggcccctgtt gattccggag agtcgcattc gaaaaatccg
     7741 agagagcgcc cccctgtgag gggccaaagc cggcggccga cgccgtcaag agaaagcagg
     7801 tggatatgaa cgcaaatgaa tggactccgg ggcagatcct cgatacttcg ggattttact
     7861 ggaagacgtg cacccttcat tgcggtgtga aactgggggt tttctccgtc ctgggggagg
     7921 agcggctggg gagtcgggag gtcgctcgaa ggctgggggc ggacccccgg gctacggcca
     7981 tgctcctgga tgcccttgcc gccatgaagc tcctggtgaa atcccgggac ggttacgcca
     8041 acacttcttc ggctcaaacg ttcctgcgca aggattcgcc ccagtacctg gggcacatcc
     8101 tgatgcacca ccatcacttg gtgaactcgt ggttccagct ggatgcggcg gtgagaacgg
     8161 gcaagtccgt gtccgggagt tcctcctcga cccgggacga agaatggagg gaggccttcc
     8221 tcatggggat gttcaacctg gccatgaaca tcgctccccg aatcgccgag gaagtggatc
     8281 tgtccggccg tcgacatttg ttggacctgg gaggcggtcc tggcacctac gccattcaat
     8341 tctgcaagaa gaacccggcg ttgagagcca ccatcttcga ccttcccacc acccggcctt
     8401 tcgcccaaaa gaccgtcgcc cggttcgggg tcgaggaccg ggtggatttc caggaagggg
     8461 actacctcga aggggagatc accgggcgct tcgatgtggc ctggctgtcc cagatcctgc
     8521 acggcgaagg ccccgagggg tgtcgatcca tcctccgaaa ggcggttgcg gccctggaac
     8581 ccgggggatt gatccttgtc catgaattca ttctcgacaa cacccgggac cgacccctct
     8641 ttccggcgct cttttccctg aacatgctcc tccagacggc ggaaggccag gcctattccg
     8701 acggagaaat tcgggagatg ttgtcggagg ccggggtcac ggacatccgc cgactcccct
     8761 tcgaagggcc caaccagtcg gggattctgg cgggggtgat tccctagaat tcagctttcc
     8821 ggaagggccg aagggttctc gaggttctct tttccgggca ggggccatca tccgcggatg
     8881 gctttgagca gccatgccat gttttcgccc aggaccttca tggtgcgaag gccttcctcg
     8941 tccttggcga cctcgccttt ttccaggccc actcccatgt tccaataaat ggaccccggc
     9001 acgatcattt gattgatgag aaagaaatga ttgatggtgt cgaaggcgtg gacggcgccc
     9061 cctctgcgta ccgccaccac cgcggcaccc acctttcttc gcagcatgct gtcgttggcg
     9121 atggccacca ggcccgcccg atcgatgagg gccttgagtt ccgccgtcac gtcggcaaag
     9181 taggtgggag aacccaggat gacggcgtcg gccgccagca tcttctcgat gcattcgttg
     9241 gcataatcct ctttgacgga gcatcgttgg tctttgttgg tgaaacactt gaaacaagcc
     9301 gtgcagcctc gaatcttcct gcccgccaat tgcaccagtt ccgtctcgat tccttccttt
     9361 tccagttcct cgaaaaccgt tttgatcaaa accgccgtgt tgccgtcttt tctcgcactg
     9421 ccgttgaatg ccaccgcttt catgccgtcg tgctccttcc tgtccgtccg taggaccgcc
     9481 gacacttgag catttgtccg agcagccctc ggaagttcac ttcatttctt tcccttgccc
     9541 ccgggcagac gttcccagtt gaggcagttg ggaggccttt gtcccgccag gccggccagg
     9601 aggttctcca cagccatccg tcccatatcc gtccgggtct cgtaggtggc gcttcccacg
     9661 tggggcaaga gcaccacgtt ggggaaatcc gccagtcccg gcgccagtgc gggttcgttt
     9721 tcataaacgt ccagtcccgc acccaggatg ttcccggcac gcagtgcctc gaccaaagcg
     9781 gattccttga ccacggggcc cctcgatgta ttgatgagaa aagccgaagg tttcatgagg
     9841 gccagttggt cccggtctat gagatggcgg gtccggggag tcaggggaac atgcagactc
     9901 acaaagtccg attccttcaa cagggtttcc agggacaccc actgggaccc gtattccgtt
     9961 tcttcctcgg gaagcagggg gttcgggttg tggtagagca ccctcatgtc gaaccccgcg
    10021 gcgcgccggg ccacggcctt gccgatacgc cccatgccga tgattcccag ggtctttccc
    10081 gcaacttgtc gacccaggaa gttgaaaggt gcccagacgg gaaacctgcc cgaacggacc
    10141 agttcgtcgc cctccaccac ccttcgggcc acggccagga tcagggcaaa ggcaatgtcc
    10201 gccgtggcat cggtgaggac ccccggggtg tagcctacag gtattccccg gcgagtggcc
    10261 gcttccaggt ccacgtggtc gtatcccaca ccgaagttgg cgatgacttt cagagcgctc
    10321 gccctctcca ggagctcttc gtcgatgcgg tcgtgaatgc tgcaaagaag tccgtccttg
    10381 tcgccgacgg catcgaggag ctctcctcgg tcgagaggac ggtcctcgag gttcatttcg
    10441 acttcatggg tcttttccac ctcggtcagc aactcccggg gcaaatgcaa ggtcacgaac
    10501 actttcatgg gaccctcccg atgacatggg tgcggattgg atttcgagcc tcggcccggg
    10561 gactcggttt ccggcttttc caccactggc gggagacttc attaccgttt cccccctcgg
    10621 gtttgcaagt cgaaattcag aggcgccgga ggcgattggc gcgaacttcg atgagttgag
    10681 cgacgatgct gacggcgatc tcttcggggg tttccgcccc gatggccagt cctatggggc
    10741 agaagaccct ttgcaggtcc tgttccgtaa atccttcccg ggtcagggcg gcataggcgg
    10801 catccctttt cttgcggctg cccatcattc cgatgtaacc cgcatgggtc ctcaagcact
    10861 gggacagaac caggccgtcg ctccggtgcc cccgggtcac gatgaccagg tagcattcgg
    10921 ggtcgatggg caaaaggtcc gtgactccct caaaggactc aagcacccgg atttcgtcgg
    10981 cggaaggaaa cctttcccgg ttggcgaatt cctcccggtc gtccagcaca acgacccgaa
    11041 agcccaccag tcggcacaag ggcgccaatt gttgggctac gtgcccgcag ccgaagatat
    11101 acacgtcccc aaggaaaccg tggggatgga cgaaatagcg aacggattcg aaatcgagaa
    11161 gccgccatgc gatcttccgg acgttccgga gaaggtcccg gggaagctct tccgagatgt
    11221 ccgaacgact cgatgcgacc gattcttccc gcaagagaaa ctggttggtg aaaagctcct
    11281 cggtcccccg aacggaggtc accacgtgga attgccgccg agcctgcagc tccgagagga
    11341 tttccccgta aaaggaccga acccgcgggc gatccccgtc gatgaaatcc accagcaccc
    11401 ggaccttgcc cccgcagacc attccacagt cttcttccgc accggacccc aggtccaggg
    11461 aaacccaggc ggggcggccg ttgtccagca cctggcggcc ccgctcttcg attaccccct
    11521 ccaggattcc gcctcccacg cttcccatca tggtgccgtc ggcccaaagg accagttggg
    11581 accccggacc ccggggaccg ggtccggatc gttccaccac cgttgccacg gccgctcgtt
    11641 tgccccgggc aacaccctca gccaggatct tctgaatttc ggcggtcgtt gtcatgaccg
    11701 aagcctcctc ggttgcgggg aagaacccgg ccgaacgggt cccctccctc catcggaccg
    11761 gatggaaccg caagaatacg ggaagcccgt gctcgaggga ccctaccggg cctcttctcc
    11821 ccggttcctt ttctcggcac agtacggacg cccgtctgcc cgtaagcttc taccacgaaa
    11881 atacgggatg caccgtattt acttaaacga ggcgaccgga gataaggggg ccatggaagg
    11941 gcgaatcgcc ggaaaccttg ctcgggaagc gggaccccgt tgccggggaa gggtttgggc
    12001 ggctttggcc gccccccgga agacgggagc tcccgttcgc cggggtgggg ctgtggggaa
    12061 ggacaccggg gggcgtgcaa acccatgaag aaatctctgg ggatcccgtt gacggtactg
    12121 ttcggcctgg catgctttgt tgccggggcc cggttcagcg ggattccggc ttcggaacat
    12181 tgccgtttgc cccacagccg cgtgctctat tacgtggacc ccctgaaccc ggctcataaa
    12241 tccgatcagc ccggcctcac tccctacggg ttgaagctcg atccggtcta cgagaacgca
    12301 agattctccc ggaacgggcc ctctcctctt gcttcgaccg gtataggggg cgggtcgggc
    12361 aaaccggaga ccatggcggt gccaacggag ccggtctccc gagcgaagga aacgtcgtcc
    12421 cctcgagtcc cgggatggtc gggcacgcgg aagcccgaca cccgggccga tgccgaagtg
    12481 cagagggaaa tcgacccggt ctgcaagatg cccgtcttgc cggaccgggc ggctcgagcg
    12541 ggcttggcgg cggattaccg ggacaagacc cactattttt gttcccggtg gtgtaaggaa
    12601 cggtttcaaa agatgcccca acaagtcttg agccgcccgg ccgagaaacc cattcccctg
    12661 gcccaatcgg ccatgcccgg tcccgtgaat cgagaagacc cggggtctca ttgaccgctt
    12721 gtcccctggg ccttgcgctg aaatgaataa ttcccgttga aaaactcatc cacgtaccag
    12781 tcgatccttc cgtcgtcgga caggaccata tccaggaggc ggacggcgcc gatgagccgt
    12841 ccgagcatgt ccaggcgcgt gaggagttcc gaacagtcgt atttcttgat ttctccgcga
    12901 accatgtccc ctttgagggt ggttttcaac cccagtctct tgaggatctt tgcgatgagc
    12961 agggcccttc gggacctgcg cccgatgtcc gccgctcccc ctttgaaaga gaaagccacg
    13021 tagttgtcgt tcacctgagg gccgcagtag gcgtcgatga cggcgaaatg gtaccccagg
    13081 cgggagttga aattgaagta ctcgttggac aggaccacgt agttggggcc tcccatccgg
    13141 tcatccatgg tcgggtcccg aaacacggat tcggccatga tggtggcgaa gcctctccaa
    13201 tccactccca ccgctcccac ccagcgcaca tccggatgag tcatgcctcg aagcagggcc
    13261 tggaagggga tcgaggtcac ctgctccatg gtggcttcgc ttgcccccgg ggggatattc
    13321 aggccgcctt gcaggtccac ggccaggatc ttcatgggaa ggggcgcctt gatgcgcacg
    13381 gcgtagcgtt tgtccatttc caccctgtcg ctgatgcgga acatctccct catggacatc
    13441 tcgtgggcaa atcggacaac gtcgtgcacc gttcggcaac cgtcggggct gaagttctcg
    13501 ttttcggggt ccgtgaggtt cagtacggcc atcttcttca aggccgcgtg acaggccttg
    13561 taggtagggc tgcccttcat gggattcacc gctttcttgc gctcgaggat gctctccacc
    13621 cgtccctgga agatgaggca gtttgttgca tccaccgtga tctcctcccc gtgggagatc
    13681 acttccgtgg cgtttcccgt tcccaccagg gtgggcaccc cgaattccct ggccaccgag
    13741 gccatgtgtc cggtgatgct tcccacgtcg gtcacgatgg cctggacccg ccccaggatg
    13801 gacacgtagc ggggcgaggt ctgcttggca atgagaatgg agcccacggg gatattgagc
    13861 aggttgtggt ccgaggtcag gacgtaggcg atgccgctgg cttttccccg ggacgccgtg
    13921 actccctggt gaaggaggat gggattgtcc ggaaacttcc tgcgcaggga ttcgaggtcg
    13981 cttcccaggg tggggacgtc cccccggacg gtcttgaggt ccacattgag cgggcgcgac
    14041 tggagcagga cgatccggtt gctcgaatcc agggcccatt cgatgtccag gggttggccg
    14101 tagtgttctt cgagcaagat ccccgcttcg gccagggaga ggagctgatc ggactcgagg
    14161 ctgggccggg ttctcagctc cggggggacc ttccgttctt ccagcccctc ggggtctgcc
    14221 aggaccagca tcgtttcctt gggggccgtc caggtggaga gaatctgccg acgggccttg
    14281 tccaccacga aaaagtccgt ggcggcggat ccctccacgg cgctgacgcc cagaccccaa
    14341 accccgttga ccaggatcac gtcccgacgg ggatcgttgg ggtcccgagt gtacaggact
    14401 ccgctggact tggcgttgac catcaccagg cacaggacgc tcatgatcac gtattcgtcg
    14461 gggtaaccct tgctgcggcg atagtaaatg gcccgggggt tgaaggtgct ggccaccact
    14521 tccttgtagg cctggagaaa attgctgcga cccaccccga ggaccgacga atgctgcccc
    14581 gcaaagctcg cctcgctatc ctcgcaggtg gcgctgctgc gaaccgccat gcggatgtcg
    14641 ggtcccagct cccggatgag agaatcgacc tcgttcaaaa cggcattctc gagatcgggg
    14701 ggcagagggg cgtcaaggat gagggattgg atcttccggc tgcactccag cagcttttcc
    14761 gtgtcctcca catccagtcc cttcaggacc cgctcggact cctcgtacaa ccggttgaaa
    14821 tgcatgaagt ggctgcaggc gtaggcggtg atcgcaaatc cccggggtac gggcaggtgg
    14881 acccggttgt acacttctcc caggctggcc gctttgcctc ccacctcggc caggcgctcc
    14941 aggctcagat cctgcatcgg cagggtgaag cgccccgcct ccaccgttct cttgcgcacc
    15001 agctcctgga gaacgctcac tccaatacgt tccaccgcat cgaaaagccg gacgaacttg
    15061 ttgttggaaa gggcattcaa ctcatcggcg attcgattga cataggaaag caggctttcg
    15121 gtctgcccga tgacgtattc cagggaaaac ggcttcggcc cgtagcaggt ctgttcgagc
    15181 tccgtcatga gctccagggc cttgttgttg gctgccagca ggttctcgaa atgctggtac
    15241 ttcttgcgaa acgccgcctg ctcgggagat tctccctcaa gggccttgcc tttccgaaag
    15301 aagtcaagaa acctcaacat atccacctcc ccgtccgttg gtcgttcgtg gtgaaaatcc
    15361 gtggtctttg ggacaaggcc ggcgccctcg gtgcttgctg cctgcaggtg cgtacaaaaa
    15421 ccttcgggag tgcgcaaaga aaagaaggtc ggtccccccg gtgcattccc ttcgggtgag
    15481 ccccggacgg acccggaacc gtcgagaacc cgatcccgtt gaagctagcg gccttgccgg
    15541 agtcttgcga ccaccgccgc gatctcccgg acggcggtgt ccacctcgtc cgccgaagtg
    15601 aaccgcccca ccgagaaacg tatggtgccc acggcgtatt caaggggaac ctgcatggcc
    15661 tgaaggaccc cggacacgca cacatggtcg ctgtgacagg ccgctcccgc ggaagccgcc
    15721 accgtccgga gttctgagag gatctgcgtg gcgctcagcc cgcggaagcc caagctggag
    15781 gtgttgggga ttcgttcccg ggggtgaccg tgcacgcgca cctccgagag ccgggctgcg
    15841 aggccttcct cgaggcggtc ccgcatccgc ctcaggtgtg cctcgtagga ggactggttt
    15901 tcccggacca tggcacaggc ctctcccagg ccgacgattt gggcaacgtt ttccgttccg
    15961 gctcgccggt tcatctcgtg gtttgccccg tcaaggagct tgtccggttg aacgccggat
    16021 cgaacgaaca gcgccccgat ccccttgggg gcgtagagct tgtgaccggc cacggacagc
    16081 aggtccactc ccagctcgtg cactcgaacg gggattttcc ccacggactg ggccgcatcc
    16141 gaatggacaa gaatcccccg ggagtgggcg atgcgggaaa tttcctccac gggttgaatc
    16201 gtcccgactt cgttgttggc gtgcataatg gaaatgagga tcgtctctgc agtgatggcc
    16261 gcttccacgt cccgggggtc cacccggccg tttccgtcca cgggaacaag ggtcgttcgg
    16321 aagccttctt cctccaggtg acggcagact tccgtcacgg ccgggtgctc gatggtcgag
    16381 gcgaccaggt gccttccccg ggaacgcagg gcgcgggcga tccctttaat ggcgtagttg
    16441 ttggattccg aacccccgct ggtgaagacg atttcttcct cccggcagcc caacatctcg
    16501 gcgacctggg tccgtgcctt gtccaccgct tccttcgcct ttcggccgta gacgtgggag
    16561 ctggaaggat ttccaaaatg ctcgagaagg taggggagca tggcctcggc cactcggggg
    16621 tcgatgggag tggtggcgtt gtagtccagg taaatgggct ttccttgaaa catctgcatg
    16681 agtttccctc tcgatccttg ggtctttcgt gcccccgatg tcctcggttt tccgatggtc
    16741 ccgcggtccc tttggtcccc tcgcgttcct ttcaa
//
LOCUS       DAOOVC010000083        16583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2356_length_16583_cov_6.238565, whole genome shotgun sequence.
ACCESSION   DAOOVC010000083 DAOOVC010000000
VERSION     DAOOVC010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2356_length_16583_cov_6.238565"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..479)
                     /locus_tag="PLM79_11195"
     CDS             complement(81..479)
                     /locus_tag="PLM79_11195"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOV86921.1"
                     /translation="MEFERRKRRHTHIDTAPLVDVVFNLLLFFVITYNVTADPAIKVR
                     LPEAATADAAAEEPLVITLERQGNVYVGEEATQREDIPRIVRQRLGEMAGPSVKIRAD
                     RDAPVGMLVAVVDGVKLAGCSAFSIVVEKN"
     gene            complement(469..1017)
                     /locus_tag="PLM79_11200"
     CDS             complement(469..1017)
                     /locus_tag="PLM79_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOV86922.1"
                     /translation="MIDFFHKGGVLMWPILCCSVVALALIVAKGFQFRGVLRRISPSI
                     DLVIREKPGEIAPVIDGIQRGLDDKQVAFIGTKQIRSLEKGLGVISLISVIAPLLGFT
                     GTVTGMISAFMTIASHGGSRVDPSMVAGGIYEALITTAGGLFVAIPSHIALHFLEDRL
                     DEIAVRMKDVAMALCERRANGI"
     gene            complement(1010..1459)
                     /locus_tag="PLM79_11205"
     CDS             complement(1010..1459)
                     /locus_tag="PLM79_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOV86923.1"
                     /translation="MASEDSHPTEQTAARHRGPESSRVRETGPQDPDARSSISDGEGR
                     AGQGPSDLFFGTGDGPRFLTKSLPRYPRLARELGKEGTVLLQLTINERGQLMNVEVLR
                     SAGSDFDEEAVRAVKNSTFKPAVRGGKPIMCRARLPIRFVLRSHGND"
     gene            2601..4814
                     /locus_tag="PLM79_11210"
     CDS             2601..4814
                     /locus_tag="PLM79_11210"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOV86924.1"
                     /translation="MEGVFGKTASRRTASCLMLGLLLACLALCRPLLASEYQEDVLSY
                     FGAGGGPGVAEYAADLGIHWSRRAVDVNENTYDSAALLSGVDQMVTENQAVGVVSLGI
                     INPRKVSGWLTAEQFSRAVQSVVERYDGDGVDDMPGLTHPIKVWELVNEYSSSGSIGY
                     EGLSQSDFLDFMVQGAQALKNTCSDCLLAYDPFDKEDSKALLQVLPADTIDLISFHTY
                     SPLDVPADPASCYYAPNFKGLLQELGIDGKPVWVTEYAFYDHEGAVFNPGYVPAGDQT
                     DNARWMVQTTAWGFGSGLFSRIIYAEIPPPKDAQTDERLLWMALLDSEGNKREIYHAY
                     KKLIALLDGFKSVTELFLGEDVYGFLFDKETERIYVLWVREGVAPRPGVLLSGLDADE
                     VTLIRSVPDSRGNFETSTLTPVEGTVSLDLENTPVFLRDAPGPTAAFSAVPTTGVLPL
                     AVEFTDLSTGAVTTHFWDFGDGETSSEQNPSHTYKTAGSYTVSLTVSGVNGSDTETKA
                     SYITVTNQPPTVTIEATDPVASESGSNRGTFTVSRTGDPGAQLEVLYEVGGSAANGKD
                     YRALSGRLTLPAGASSATIRVIPVDDNVRESDETVTLSLLADDSYEVGTPSEATLTIG
                     DNDPTVTIEATDPVASEKGKDPAVFTVRRTGNRRASLTVVYSIQGTAKNGVDYSKLSG
                     RVKIPEGKASASVRVVPLRDDRKEGNETVILTLVESSSYHVGSPSVAKAVIRKDD"
     gene            5877..6635
                     /locus_tag="PLM79_11215"
     CDS             5877..6635
                     /locus_tag="PLM79_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOV86925.1"
                     /translation="MIDLGWLGQISFTWEFFAAVMSIVLIDLVLAGDNAVVIAMAVKN
                     LQGRKRRMGIILGSGGAVVIRVVCTFLVARLLNMSFVKLVGGAVILWIAVKLLTEGTR
                     EECRGKECSSVLQALWIIIVADLSMGIDNMLAVGAASHGNLFLLLFGLILSIPMVVFL
                     SAWLSNLMDRYPIILWLGAAVLGKVGGEMMITDPWVRGLLNPPKWTEYAVMAFFVVFV
                     CMLSKWIINRRKGGVVPEPASVQASVVSSNPEAA"
     gene            7125..8135
                     /locus_tag="PLM79_11220"
     CDS             7125..8135
                     /locus_tag="PLM79_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86926.1"
                     /translation="MKSGSNDRCLCTGGTKLKECCVDKRKADERQAMLDAAVREVREL
                     LESRSFASLEEANAFLNRHMQQRNRAPEDDFDGLSSGQMHRLLHYPFESPDLAAFPAC
                     LDVVPDAPILRLFSLLVEKLGDQGLKATTTGNLPRNLCREIALTYWGEEKYREVFRYG
                     ELRTEPMFFDLHVTRIVSGLSGLVRKYRGKFIVSNGCRRLLNKQGLAGIYPRLFREFA
                     TKYNWAYWGGRSEIPLIQQSFLFTLYLLRRHGTEWRSCRFYEDWFLRAFPAVLNEVVP
                     IGEYLSPEKVLRGSYSWRCLDRFAGFLGLVEIRRDPGKGLRGDFLVRKLPLLDHVVQF
                     HL"
     gene            8166..8444
                     /locus_tag="PLM79_11225"
     CDS             8166..8444
                     /locus_tag="PLM79_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86927.1"
                     /translation="MDPLHDLPAAAKPETLTRLIARLADLRPELRRECFDDPKKHVTL
                     TTEQKILSEGEIVMALWGELYPDLEDPDSYGGGEEEFAKVIPGCGKPV"
     gene            8667..10232
                     /locus_tag="PLM79_11230"
     CDS             8667..10232
                     /locus_tag="PLM79_11230"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOV86928.1"
                     /translation="MGGREKNPGSFREVRGSRRVSWAVLILGWVFLSQPVCWAKPTTA
                     GQAETVVRNWLRLDPQPLGAPLGGPGKEVRTVCDKEGRDLFHVVSLHPTGFVIIAGDD
                     LVEPVIAFVARGRYDPSETNPLTAIVTEDMTRRLDLVRTIPVEAAKEESGGAARGWME
                     ASLEKWVFLQRARGGERPEVSSGLEKVSDLRVAPFVSSKWGQGEEGSEYCYNYYTPKH
                     YFCGCTAAAMAQLLRYHRHPSDLVGKKRFRIAVDGKNSYATTRGGDGAGGAYAWARMP
                     LNPGPATGEEERRAIGALTYDAGVTIHTRYAGAGSAAGLPDVREALDSFFQYSNARYG
                     SSPTGPISLKDLCPMINPNLDAKLPVLMSVIRSGGAHVVLSDGYGYNLSTLYHHLNLG
                     WNGSDDAWYHLPNIPTSRYRYSLIADVVYNVFPTGTGEIVSGRVTNAGGEPLKGVRVR
                     AEVNGSLVGSAHTDRYGIYALPGLPSKSSIELRLNKPGYTFRPRTVETGRTDDFRVRT
                     GNLWGVNFSARSQ"
     gene            complement(10607..12568)
                     /locus_tag="PLM79_11235"
     CDS             complement(10607..12568)
                     /locus_tag="PLM79_11235"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV86929.1"
                     /translation="MRKLTLGTRLTLGGIVIVVVPLLIIGLFAIMKASNALTALSREQ
                     AANVTAKLADMTQVALSSELKVVKELSQEPLIIDTAAKVAAANAEEYGADIQRLNRRL
                     SGIMKQIGNEYESILVTNSKGGIVADGSNGEYANLSISNRDYFLAAGKGKTNVGTVVK
                     SQKTGNPVAPICAPIYGPTGEFLGSVSIPLKIDFLVEKIAATKMGATGYAFMTDAGGR
                     VIAHPRRDLILELDLKNAKGMESIVSNMLAQKAGVESYVFEGIPKIAGYAPVGLTGWS
                     VAVTQPDEEFLAPAHAIRNGIFLVGIVFLGLTLVLVFFFARSISRPIARVVQGLGEGS
                     HQVAAASAQVSASSQSLAEGASEQAASLEETSSSLEEMASMTKQNADHANQANSLMVE
                     MTQVVKESNAAMAQLTTSMSEISRASEETSKIIKTIDEIAFQTNLLALNAAVEAARAG
                     DAGAGFAVVADEVRNLAIRAADAAKNTANLIEDTIKKIKEGTEQVNKAGDAFGLVASS
                     ATRMGELVSEVAAASNEQAQGISQVNKAVAEMDKVVQQNAANAEESAAASEELNAQAE
                     QMNAYVADLVAVVGGNGKKGGNGRMVLTARAGNAGENGKSMSPAFGLTRDPGHTGKRQ
                     IGKQRAVSRELEVRPEQVIPLGEDEMGRF"
     gene            complement(12786..14678)
                     /locus_tag="PLM79_11240"
     CDS             complement(12786..14678)
                     /locus_tag="PLM79_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOV86930.1"
                     /translation="MRDRDKTKVQLIQELKELRRWVADSKARQPRNTPATPSFDTTAA
                     RLNAILKAFDGFVYVCSRDYRIEFMDERLVRRKGYDGTGGLCHRVLHERDSVCPWCAN
                     EHVFRGETVRWETRGPKDDRWYDVISAPIVNPDGTLSKLELIRDVTERVWHEREIKLL
                     NRLYRVLSRVSQAVVRATSPEVFLEEACRVIVDEGGFLLAWIGYVDEESHRVVPTAIW
                     GGAYDYAHGISVYADDRPEGRGPTGSCIRHKRPFVYNDFLHDQPTKPWHERAAGFGIK
                     AAAAFPIESGGRVWGALTIYSDEIGFFGLEDVKLLEKVAGDIGFALDNLERERRRQKA
                     EAELRESQQQLSYIIDFLPDATLVVDKERKVIAWNRAVEEMTGVRKEDILGKGDYAYA
                     VAFYGKPRPMLLDMVVTGGAEPAAGYDFVERKGNQLRAEGHAPNAFQGRGAYVWGTAA
                     PLYDREGNIAGAIESIRDISKRKAMESALQQREKALENKTHQLEEMNAALNVLLQKRA
                     DDEKELQESVLANLKEMVLPYLDKIKQSRLDETQRTYIGILESHLNEVLSPFLKNLST
                     KFTGLTPTELQVAGLVKDGKTTKEIARLLGVAPKTVSAHRYNLRVKLGLKNEKINLRS
                     HLLSHG"
     gene            complement(14747..15175)
                     /locus_tag="PLM79_11245"
     CDS             complement(14747..15175)
                     /locus_tag="PLM79_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOV86931.1"
                     /translation="MADVFSEQQRSWIMSRIHGSNTRPELQVRKVLHAMGFRYRLHYS
                     KLPGKPDIALPGRKKAIFVHGCFWHGHPDCPRSKLPSTNVEFWKAKIGRTMERDRENA
                     EALARLGWEVLVLWSCETRRRKVLIQKLEDFLNGPIEPEQ"
     gene            15781..>16583
                     /locus_tag="PLM79_11250"
     CDS             15781..>16583
                     /locus_tag="PLM79_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02452 family protein"
                     /protein_id="HOV86932.1"
                     /translation="MDEPGISRELAARYGRQAVEIIESGEYVSPSGRVVQIGHRVKRS
                     VEGTVSYPPDRPFQESARGPFATGITVQNTTTLEAARTLIVAGDFPAVLNFASATHPG
                     GGFLSGARAQEESLARSSALYACIRNDPMYVFHRSLRDPLYTNYAIYSPDVPVFRADD
                     GSLLEGPYTVAIITSPAVNAVRLDPARRNEIGPAMWRRILKVLSIGLHHGHDALVLGA
                     WGCGAFGNDGREIAGLFHRALEDNFKGAFRHIVFAVLDRSRGRKVIGPFR"
BASE COUNT         3126 a         4997 c         5064 g         3396 t
ORIGIN      
        1 tgtcagtctc acgcgagatg tagggcttcg ctttaaaccg ggatgaccag agagcgggcg
       61 ggcggatgaa cctctcttct tcaattcttt tccacaacga tgctgaaggc cgagcagcct
      121 gccagcttaa caccgtcgac cacggcgacg agcatcccca ccggtgcgtc ccggtccgcc
      181 ctgatcttga cggagggtcc cgccatttcg ccgagcctct ggcggacgat ccgggggatg
      241 tcttctctct gtgtcgcctc ttcccctaca tacacgttcc cctggcgctc cagggtgatg
      301 accaggggtt cctcggccgc ggcgtcggcc gtagccgcct ccggaagccg gaccttaatg
      361 gccgggtccg ccgtcacgtt ataggtgatc acaaaaaaga gaagcaggtt gaagaccacg
      421 tccaccaggg gggcggtgtc gatgtgggtg tggcgcctct ttcttctctc aaattccatt
      481 cgctctcctc tcgcagagtg ccatggcaac atccttcatg cgcacggcga tctcatccag
      541 ccggtcttcc agaaaatgca gggcgatgtg gctgggaatg gccacaaaga ggcctccggc
      601 cgtcgtgatg agcgcctcgt agatgcctcc ggccaccatc gagggatcga cccggcttcc
      661 gccgtggctt gcgatggtca tgaaagccga gatcatgccc gtcacggtgc cggtaaagcc
      721 cagaaggggg gctattaccg atatgagtga gattacgccc agccccttct cgagacttct
      781 tatctgttta gtgccgatga aagcgacctg cttatcatcc agccctctct ggatgccgtc
      841 aatgacaggg gcaatctctc cgggcttttc ccggatgacc aggtcgatcg aaggggaaat
      901 ccgtcgaagg acgcctcgaa actggaatcc tttggcaaca atgagggcca gcgcgaccac
      961 agagcagcaa aggatgggcc acatgaggac cccgcctttg tggaaaaaat caatcatttc
     1021 catgactcct caaaacgaat cgaataggca ggcgggccct gcacataata ggcttgcctc
     1081 ctctcacggc aggcttgaag gtggagttct tcaccgctcg aacggcttct tcatcgaaat
     1141 ctgatccggc cgatctcaac acctcgacgt tcatgagctg ccccctctca ttgatggtca
     1201 actgcagaag cacggttcct tccttgccga gctcacgggc cagccgggga tacctgggaa
     1261 gagatttggt cagaaagcgc ggcccgtcgc ccgttccgaa aaacaagtcc gaaggtccct
     1321 gaccggccct gccttctcca tcggaaatcg acgaacgagc atctgggtct tgagggccgg
     1381 tttctctcac ccggctggat tcgggtcctc gatgccgggc ggccgtttgt tctgtgggat
     1441 gggaatcctc cgaagccatc tggggctgag actgcacccc cgccggcttt tctcgccgag
     1501 ggggcatcac cgggcgcact ggtggtttct tgaccggctg aagcggcttc gggacttccc
     1561 cggcaagcag ggtcggccaa ggggtgtcct cggcaagagg aagtggttgg gtcctggggt
     1621 ggttgtcctt ggattcctcc ggttgggggc ccgagatcgg cagagacgga gcgggagccg
     1681 ttggcggctt gttgccggcc gttgcgggat tctgcgtctc cgccgacgtg ctccctttgc
     1741 ggccctcctg aacaatgaca aaccgcaggg cctcgcaagg acgttccgga gggagtctct
     1801 caggaccggg gacgaggaag acacccgcgt gaaacagaac ggaaaacaag ataccttgcc
     1861 agagcttcat ccgaactgat gaaatcctcc cccgaagttt ctcccccggg gtggccgtga
     1921 aaaaagccgc gacttccccg gttcgccgca ttcgcacggc ttctggggcg ttcgcggccc
     1981 tgtcggtttc agatcggacg aactcccggc cgtgatctgc agcgagcctt cccggccggg
     2041 tccaaaggaa atcggcactc aggggtcccg gttggtatga caccttcatg gtgcactgta
     2101 gcctgggccg acagggccta ttatcgacgc cggtcgcctt gcgtcaagcg atttccgaca
     2161 taccccggtt ttgcctcatg cagagttgcc tccgcccgat cgactcactg ctcctctcga
     2221 ttccgcaaac cgggcccgac ggtttcctct cgtcagaaac ccggcggacc cggaacctac
     2281 cgtggctgta cggcgatgaa tccggcttcg tggaagactg gcgggtgcag tcggccgaga
     2341 gcggtcgcca cccgagtgag acaaggtttt cccaaggaag atttgcggtc gggaaggttc
     2401 ttaccggtcc cctacggcga ccgggtcctc atggttcccc cgacgggaca aggatcaagg
     2461 agactggttg cggccgttga cggtccccga atcatcgctc ccctattgaa gactgttgac
     2521 tcccaacggc agtcgggact acaaaaagcg aattcatcga agtctcgtcg gtcggcggac
     2581 ggatctgata aggatacgac atggaaggag tgttcgggaa aaccgcctct cgtcgcacgg
     2641 cctcctgcct gatgctgggc ctcctgcttg cctgcttggc cctttgccgt cccctcctcg
     2701 catcggaata ccaagaggat gttctctcgt atttcggcgc tggtgggggg ccgggagttg
     2761 cggagtacgc tgccgacctg ggcattcact ggtcccgaag ggccgtcgac gtcaatgaaa
     2821 acacgtacga cagcgcggcc cttctttccg gagtggacca gatggtgacc gagaaccagg
     2881 cagtgggggt ggtcagcttg gggatcatca accccaggaa ggtgtccggc tggctcacgg
     2941 ccgagcagtt ctcccgggcc gtgcagtccg tcgtggaaag atacgacggg gacggcgtgg
     3001 acgacatgcc gggtctgacc caccccatca aggtatggga gctggtcaac gaatactcga
     3061 gctcaggcag catcggctac gaaggactgt cccaatcgga cttcttggat ttcatggttc
     3121 agggggctca ggccctcaag aacacctgct ccgattgcct gctggcctac gacccctttg
     3181 acaaagagga ctcgaaggcc ctgctgcaag tgctcccggc cgatacgatc gatctcattt
     3241 cctttcacac ttacagtccc ctggacgtgc cggccgatcc ggcctcttgc tactacgcac
     3301 ccaatttcaa gggactcttg caggagttgg ggatcgacgg aaaacccgtc tgggttacgg
     3361 aatacgcctt ttacgaccac gaaggcgccg tgttcaatcc cggttacgtg cccgccgggg
     3421 accagacgga caacgcgcga tggatggtgc aaaccaccgc atgggggttt ggatcgggac
     3481 tcttctcaag gatcatttat gcggaaatcc ctccccccaa ggacgctcag accgacgaga
     3541 ggcttctgtg gatggcgctt ctggactccg agggcaacaa acgggaaatc tatcacgcct
     3601 ataagaagct catcgcattg ctggacggct tcaaatccgt gaccgagctt ttcctggggg
     3661 aggacgtcta cgggttccta ttcgacaagg aaaccgagcg gatctatgtc ctatgggtga
     3721 gggaaggggt cgcccccaga ccgggagtcc ttctttcagg gttggatgcc gacgaggtca
     3781 ctctcatccg gtcagtgccc gattcccggg ggaatttcga gacgtccacc ctcacgcccg
     3841 tcgaggggac cgtatccctc gacctcgaaa acactcccgt cttcctgcgc gatgctcccg
     3901 gtccgacggc cgctttcagt gccgttccga ccacgggggt ccttccgctg gccgtggagt
     3961 tcacggattt gtccaccggt gccgtcacga cccatttctg ggattttggg gacggagaaa
     4021 ccagctcgga acaaaacccg agtcacacct acaagaccgc ggggtcctac acggtgagcc
     4081 taacggtgtc gggtgtgaac ggttcggaca cggaaaccaa ggcttcctat attacggtaa
     4141 ccaatcaacc gcccaccgtg accattgagg ccacggatcc cgtcgcctcg gaatccggct
     4201 cgaaccgagg gaccttcacc gtgtccagga ccggggatcc cggggctcaa ctcgaggtcc
     4261 tctacgaggt gggcggaagt gccgcgaacg ggaaggatta ccgggcgctc tcaggcaggc
     4321 tgacgctccc tgccggagcc tccagcgcga ccattcgagt gattcccgtg gacgacaacg
     4381 tgcgggagtc ggacgagacc gtgacccttt ccctgcttgc cgacgattcc tatgaagtgg
     4441 gcactccctc cgaagccacc ctcaccattg gcgacaatga tcccaccgtg accattgagg
     4501 ccacggatcc cgtcgcctcg gaaaagggca aggatcccgc cgttttcacg gtgcgccgca
     4561 ccggaaatcg aagagcctcg ctcaccgtgg tctactccat ccagggtact gcgaaaaacg
     4621 gcgtggatta ctcgaagctc tcgggtcggg tgaagattcc cgaaggcaaa gcctccgcct
     4681 ccgttcgagt ggttccttta agggacgaca ggaaagaggg caacgagacc gtgatcctga
     4741 ccctggtgga aagcagctcc taccacgtgg gctctccctc tgtggccaag gccgtcattc
     4801 gcaaggatga ctaatgcctg agccgaacga ttcttgccgc ccatccccca tccaacggcg
     4861 ctccccacag acgcggacga tccctcgagg atcggttcac ccgtcaccga gagcgggctt
     4921 cgtgcctgcc ctccgaggcc ctgcaaccgc aggaaatcgg tcatttgggg cagtgtccgg
     4981 caggttcgtt tccccggggg tgggccgccg gggtgcaaga ccccggtctt cggaacgttc
     5041 cgggggggac cccgcttcac tcctgcaatc cggaggtttg tacccatccg gaagaaccat
     5101 gtcgactcgg ggaccgggca tcccgagaaa agacccacca agaacccctt cctggtttcg
     5161 atggatactt tgcattcctg agaaccgggc gggattcgcc aggaacgagc acggcaggag
     5221 actctcgaga ttcttgattt cccttctcga cgtctggatg caaaagaaaa ggtccctgat
     5281 ccggacgaag tcgaggccaa gtacgggcac acggtggcct gaagattttc ctgcccgggg
     5341 cagagcatgc aaagccccag aagataccgg tcctggcggg gcaaaggacg gaggcctcaa
     5401 tggccatcgt cgatctttcc gcttggagcc catgaggaga tgaaaccgcg gccggcaagg
     5461 gacttcgcct tgtcgaggag aaggaagtcg gcgggggtgt ggtttctccc cggctgaatg
     5521 gaccaaggtt ccagggactc cggattgagt gaagctgcaa cccctcccct ttgtctgcag
     5581 agggaaccgg gccttgaaac cttcggggaa accatggggg gaacactttc catgatgaga
     5641 accccgttga caggtccctg cggtttcact agtttggtgg aaatcgtaaa ggggagtagc
     5701 aaccggccga cgggcggccg gaccgtgtcc cgtcactacg gtggcgattt ccacccggga
     5761 gcggatccaa gcaacagtgg ttggacttgc aagaccttta ctcacagcat gggccgtcat
     5821 gccgcgagtg agggtctttt tgtttggacg cggcgaaaac gaaacgagga gtcaacatga
     5881 tcgacctggg ttggttgggg cagatttcgt tcacttggga attcttcgca gccgtgatga
     5941 gcatcgttct cattgacctc gtcctggcgg gggacaacgc cgtggtcatt gccatggcgg
     6001 tcaaaaacct gcagggtcgc aaacgccgga tggggatcat cctgggttcc ggcggggcgg
     6061 tcgtcatccg ggtggtctgc acctttctgg ttgcccggtt gctcaatatg tcctttgtca
     6121 agctggtggg cggagcggtc atcctctgga tcgccgtaaa gctgctcacc gaagggaccc
     6181 gggaggaatg ccgcggcaaa gagtgcagca gcgtcctgca ggccctctgg atcatcatcg
     6241 tggcggacct cagcatgggc atcgacaaca tgcttgccgt gggcgccgcc agtcacggaa
     6301 acctgttcct gctgctcttc ggcctcatcc tttccattcc catggtggtg tttctgagtg
     6361 cttggctctc gaacctcatg gatcgctacc cgatcattct ttggctgggt gcggcggtgt
     6421 tgggcaaagt gggcggtgag atgatgatca ccgacccatg ggtgcgcgga ctgctcaacc
     6481 cgcccaagtg gaccgagtac gccgtcatgg ctttctttgt cgtctttgtc tgtatgctga
     6541 gcaaatggat catcaacagg cgaaaaggcg gggtcgttcc ggaacccgcg tcggttcaag
     6601 cctcggtcgt ctcatcgaac ccggaagccg cctgatcgac gagtcctacg aaatcaaccg
     6661 gcggggagga acgggtcgtt cctccccgcc ggtcgggttg cctctcttcc ggcccgcagg
     6721 ggcctccttc tcaatttcca aggcaagagc ttccccctga aaacggcgtt cgtccggttg
     6781 agcgaaaaca gaaaaaatct tccgggtgaa aacggaatag gaatggatct ccggaccgat
     6841 tcacggtagc ctgtcttttt attgtcgccg atgaactccg tctcgaccac gggagttggg
     6901 gtcttccgcc agccgcccgg gggcgggtcg aggggcagag ctatgtggaa accaagaccg
     6961 accttgggcc ggattacctg agacattacc gatggaaaac agcaccggaa ccggctgaat
     7021 acgtcttgag gcgaaacctt gcttcatccc cctcgcaagg gaggaagcgt aggtcgtgcg
     7081 ctcaaatgtc ttggtaaggc catcgaggcg ctgaggaggg aatcatgaag agcggatcca
     7141 atgatcgctg cctctgtacg ggcggaacaa aactcaagga atgctgcgtc gacaaacgga
     7201 aagccgatga acggcaagcc atgcttgatg cggcggtaag agaggtgagg gagctgctcg
     7261 aaagccgcag ctttgcctcg ctggaagagg ccaatgcctt cttgaaccga cacatgcaac
     7321 agcgcaaccg ggcgcccgag gatgacttcg acggcctgtc gtccggacag atgcaccgtt
     7381 tactgcacta cccgttcgag tcgcccgacc tggcggcttt tcccgcctgc ctggatgtgg
     7441 tccctgacgc tccgatcctt cgtctcttca gcctgttggt tgaaaaactc ggcgatcagg
     7501 gtctaaaggc gaccactacc ggcaatctcc cgcgtaacct gtgccgggag attgccctga
     7561 cctattgggg agaggagaag taccgggaag ttttccgtta cggggaactg cgaacggagc
     7621 cgatgttttt tgatctgcat gtcacccgaa ttgtttccgg actgagcgga ttggtaagaa
     7681 agtacagggg aaaattcatt gtgagcaacg ggtgccggag gttgctgaac aagcagggtt
     7741 tggccgggat ctacccgcgg cttttccgtg aatttgccac aaaatacaac tgggcctatt
     7801 ggggcggacg gagtgaaatc cccctgatcc agcaatcctt tctgttcact ctctacctgc
     7861 ttcggagaca tggaaccgaa tggcgatcgt gccggttcta cgaggattgg ttcctgcgag
     7921 ccttcccggc ggtgttgaat gaggtcgtgc caatcgggga atatctctcg ccggaaaagg
     7981 tcctgcgcgg ttcttattca tggcgctgcc tggacaggtt tgccggcttc ctgggactgg
     8041 tggaaatccg gcgggatccc ggtaagggtt tgcgcgggga ttttctcgtg agaaaacttc
     8101 cactcctgga tcacgtcgtc caatttcatc tatgaagtga gaagacgtcc aagaggaaag
     8161 aatcggtgga tccgctccat gatttgccgg cagcggccaa gcccgagaca ttgacccgct
     8221 tgatcgcccg ccttgccgat cttcgtcccg agctgcgccg cgagtgcttc gatgatccga
     8281 aaaagcacgt caccctcacc accgagcaga agattctctc ggaaggggaa atcgtaatgg
     8341 cgctctgggg ggaactgtac cccgatctcg aggacccgga cagctacggc ggcggcgagg
     8401 aggagttcgc aaaggtcatt ccgggatgtg gaaaaccggt ctgaggcgga tgccgcgcga
     8461 cttccttcgg ccgtgctttt ggccgccggg cggccccccc gcaggattcc ggcagcaaag
     8521 acggtcttgc ccaccgccgg aggttgtgta gggttggcct ctgcgggtcc gtggcggtct
     8581 ttggcgggtc ggcccccaac cccgggtgga ccgggaagac gtcgtatccc gggggtcaac
     8641 gattccggca aggaaaggga ttggaaatgg gaggtaggga aaagaatccg ggttcattta
     8701 gggaggttcg aggcagccgc cgcgtctcct gggccgtttt gatcctaggg tgggtgtttc
     8761 tgtcccagcc ggtgtgctgg gccaaaccca ccacggcggg ccaggcggaa acggtggttc
     8821 ggaactggct ccggctggat ccgcagcccc tgggcgcccc cctgggcggc ccgggaaaag
     8881 aggtgagaac ggtctgtgac aaggaaggaa gggatctttt ccacgtggtc tccctccatc
     8941 cgaccggctt cgtgattatc gcgggcgacg acctggtgga gcccgtgatc gccttcgttg
     9001 cccgaggccg gtacgacccg tcggagacaa atcccctcac ggccatcgtg accgaggaca
     9061 tgacaagacg cctcgatctg gtaagaacga taccggtgga ggcggcgaaa gaggagtccg
     9121 gcggagcggc ccgagggtgg atggaggcga gcctggaaaa gtgggtcttc ctgcagcggg
     9181 ctcgcggggg ggagcgcccg gaggtgtcct ccggactcga gaaggtctcc gatctgcggg
     9241 tggctccttt cgtgtccagc aagtgggggc agggggagga aggatccgag tactgttaca
     9301 attactacac cccaaaacac tacttctgcg gttgtaccgc cgcggccatg gctcaactgc
     9361 tccgttacca tcggcacccg tcggacctcg tggggaagaa aaggttcagg atcgccgtag
     9421 acggcaagaa ttcctacgca accacccggg gaggcgacgg ggcgggggga gcctatgcct
     9481 gggcgcgaat gcccctaaac ccgggtccgg ccacgggcga ggaagaaagg cgggccatcg
     9541 gcgccctgac ctatgacgcc ggggtcacga tccatacgag atatgccggt gcggggtccg
     9601 ccgccggact cccggatgtg agggaagcac tcgattcttt ctttcagtat tccaacgccc
     9661 gctacgggtc ttcaccgacc ggccccattt ccctcaaaga cctctgcccc atgatcaatc
     9721 ccaacctgga tgcgaaactg ccggtcctga tgagcgtcat ccgctcgggg ggagcccacg
     9781 tggtgttgtc ggacggatac ggatacaacc tctcgaccct gtaccatcac ctgaacctgg
     9841 gttggaacgg gagcgacgac gcatggtacc acctgcccaa tatccccacg tcccggtacc
     9901 gttacagcct gattgccgac gtcgtctaca acgtatttcc cacggggacc ggagaaatcg
     9961 tgtcggggcg cgtgaccaac gcgggcggcg agccgctcaa gggggtaaga gtgagggcgg
    10021 aagtgaatgg gtccctcgtc gggtcggctc acaccgaccg gtacggaatc tacgctttgc
    10081 cggggttgcc gtccaagtcg tccatcgagc tccgcctgaa caagcccggg tatacgttcc
    10141 ggcctcgaac cgtcgaaacg gggcgaaccg acgattttcg cgtccggacc ggcaaccttt
    10201 ggggggtgaa tttttcagcg cgttcccagt gaaggggagg acccgggcca cccaacaggg
    10261 gcctccccgg cgccgaaccg ggcggccaag aaaagtgcag gaagcgcttg cgcaggtgtg
    10321 ccgggtggtt cgttgtccag gggtccctgt ccatgttggc aacgaagata gcgggtttgc
    10381 ccgccaaaga acccggcctt ctcctttcac cctgcgccgt ctttcccatc cggaacactg
    10441 cggatgagct cgaccgtgcc tcttgggcta aggggtttcc aggctgggtg ctctccctcg
    10501 cacatccatc gggtggactg cgtgaaaccc actgtggaga caaccctcgg gaagcagggg
    10561 aggggcggaa caaatgaatc cccacccccc ccatcgaggc ctgctcctag aaccgtccca
    10621 tctcgtcttc tcccaacggg atgacctgtt cgggccggac ctccagttcc cgggaaaccg
    10681 cccgctgttt cccgatttgt ctttttccgg tatgtccggg gtcccgcgtg agcccaaagg
    10741 ccggcgacat gctttttcca ttttccccgg cgttcccggc ccgggccgtc agcaccatgc
    10801 ggccgttgcc ccccttcttt ccgttcccgc cgaccacggc cacgaggtcc gccacgtagg
    10861 cattcatctg ctcggcttgg gcgttgagct cttcggacgc cgcggccgat tcctcggcgt
    10921 tggcggcatt ttgctggacc accttgtcca tttccgccac cgccttgttg acctgggaaa
    10981 tcccctgggc ctgttcgttg gatgcggcgg caacctcgct caccagttct cccatccggg
    11041 tcgcgctgga ggccaccagt ccgaaggcat ccccggcctt gttgacttgt tccgtaccct
    11101 ctttgatctt cttgatcgtg tcctcgatca ggtttgccgt gttcttggca gcgtcggcgg
    11161 cgcggatggc caggttgcgc acctcgtcgg ccaccacggc aaacccggcc cccgcatctc
    11221 ccgctcgtgc ggcttccacg gccgcgttca gggccaggag gttcgtctgg aacgcaattt
    11281 catcgatggt cttgatgatc ttcgaggtct cctcgctggc ccgggaaatc tcgctcatgg
    11341 aggtggtgag ctgggccatg gccgcgttgg actccttcac tacctgggtc atctccacca
    11401 tgagactatt ggcctggttg gcatgatcgg cattctgctt cgtcatggaa gccatttctt
    11461 ccagggacga ggaagtctct tccagggagg cggcctgttc ggaggcccct tccgcaaggg
    11521 actggctgct tgcggatacc tgggcggagg ccgcggcaac ctggtgggag ccttccccga
    11581 gaccctggac cacccgggcg atcggcctgg aaatactgcg ggcaaagaag aataccagga
    11641 ccagggtcag tcccaagaaa acaatcccta ccaggaaaat gccgttgcga atggcgtggg
    11701 cgggggccag gaattcttca tccggctgag tcacggccac gctccaaccg gtcaggccca
    11761 ccggcgcgta tcccgcaatc ttggggatcc cctcgaaaac gtaggactcg actccggctt
    11821 tttgagccag catgttgctc acgatgcttt ccattccctt ggcgttcttc aggtccagct
    11881 cgaggatgag atcccttctg gggtgagcga tcacgcgccc gcccgcatcg gtcatgaagg
    11941 catagccggt ggctcccatt ttggttgcgg caatcttttc caccaggaag tcaatcttca
    12001 gaggaatgga gacggacccc agaaactcgc ccgtgggccc gtagatggga gcgcagatgg
    12061 gggccacggg gttgccggtc ttttgggact tgaccacggt cccgacgttg gtctttccct
    12121 tcccggcggc caggaagtag tcgcggttgg aaatgctgag atttgcgtat tccccgttgc
    12181 tcccatcggc cacgatccct cccttggagt tcgtgaccag gatggattca tactcgttgc
    12241 cgatttgttt catgatgccg gagaggcgcc gattcagcct ctggatgtcc gccccgtact
    12301 cctccgcgtt ggctgcggca accttggcgg ccgtatcaat gatgagcggc tcctgggaca
    12361 attccttcac cactttgagc tcgctcgaaa gggcgacctg ggtcatgtcg gcgagcttgg
    12421 cggtcacgtt ggcggcctgt tcccgggaaa gggccgtgag ggcgttggaa gccttcatga
    12481 ttgcgaaaag gccgatgatc agcagcggca ccaccacgat gacgatgccg cccagtgtga
    12541 gtcgtgtacc cagagtcagt ttcctcatga ttccctcgat cctctcttct tacgtagtca
    12601 acggatgccg gtcgaattgg ttttggggca gtcgccggtc cgggccgatg aagtcgcctc
    12661 ggttggtgct ttgcgtctac ccgggaaacg caagcagcac ggctgcctgc tggggactat
    12721 cggcagaaga aaggaaaaag tcgataccgg aaaatcacct atggaatgcg tagaaagggt
    12781 gggtactacc cgtgagaaag aaggtgggat cgaagattga tcttctcgtt cttcaatccc
    12841 agtttcacgc gcaggttgta gcggtgggcc gagacggtct tgggggcgac tcccagcagc
    12901 cgggcgattt ccttggtggt cttgccgtct ttgaccaggc ccgccacctg gagttccgtg
    12961 ggagtgaggc ccgtgaactt ggtggagagg ttcttcaaga agggggacag gacttcgttg
    13021 aggtgagact caagaatccc gatgtaggtc ctttgggtct catcgagccg gctctgcttg
    13081 atcttatcga ggtaaggcag gaccatttcc ttcaagttgg ccaggacgct ttcctggagc
    13141 tccttttcat cgtccgccct tttctggagc aacacgttca aggcggcatt catctcctcg
    13201 agttgatggg tcttgttttc cagcgccttc tctcgttgct gcaaagcgga ctccatggcc
    13261 ttgcgcttgc tgatgtcgcg gatggactcg atggcccccg caatatttcc ctcccggtcg
    13321 tagagagggg ccgccgtgcc ccaaacgtag gcgccccttc cttggaaggc attgggggca
    13381 tgcccttcgg cacggagctg gttccccttc cgctcgacaa agtcgtaacc ggcggcaggc
    13441 tcggcgcccc ccgtcaccac catatcgagg agcatgggcc ggggttttcc atagaaggca
    13501 acggcatagg cgtaatcccc cttcccgagg atatcctctt tcctcacgcc ggtcatctcc
    13561 tcgacggccc ggttccaggc aatgaccttt cgttccttgt cgacgaccag ggtggcatcg
    13621 ggcaggaaat cgatgatgta ggacagctgc tgctgggatt ccctcagctc ggcttcggcc
    13681 ttttggcgtc ggcgttccct ttccaggttg tccagggcaa agccgatgtc tccggccacc
    13741 ttttccagga gtttcacatc ttccaggcca aagaacccga tctcgtcgga atagatggtc
    13801 agagcgcccc acacccgtcc ccccgattcg atggggaaag cggcggcggc cttgatcccg
    13861 aaacccgcgg cccgctcgtg ccacggcttg gtgggctggt cgtgcaggaa gtcgttgtac
    13921 acaaacgggc gcttatggcg aatacaactg cccgtgggcc cgcgcccctc gggacgatcg
    13981 tcggcgtaca cggaaatgcc gtgggcataa tcgtaggccc cgccccaaat ggctgtgggc
    14041 acaacccggt ggctttcctc gtccacgtag ccgatccagg ccaggagaaa gcccccttcg
    14101 tccacgatca cccggcaggc ctcttccagg aaaacctcgg gggaggtggc cctcaccacc
    14161 gcctggctca cccggctcag cactcggtag aggcgattga gcaatttgat ctcccgctcg
    14221 tgccacactc gttccgtgac gtcccggatc agctccagct tggatagagt gccgtccggg
    14281 ttgactatgg gggcgctgat cacatcgtac cagcggtcgt ctttggggcc ccgggtttcc
    14341 caccgaacgg tctctccccg aaatacatgc tcgttggcgc accacgggca aacggaatcc
    14401 ctctcgtgga ggaccctatg gcagagcccg cctgttccat cgtatccctt tcgccggacg
    14461 agccgctcgt ccatgaactc gatgcgatag tcccgcgaac aaacgtaaac gaacccgtcg
    14521 aaggccttca ggatggcgtt caaccgggca gcggtcgtat cgaaggaagg ggtcgcaggc
    14581 gtgtttcggg gttggcgagc cttcgagtcg gcaacccacc ggcgcaattc cttcaattct
    14641 tgtatgagct gcactttggt tttatcccgg tctctcatgg ttgaatttcc tggaaacaac
    14701 tccctcgttc cttgaactgc ccacgggatc gggcaggagg gaggaatcat tgctcgggct
    14761 cgatcggacc gttgagaaag tcctcgagtt tttggatcag aaccttcctg cgccgggtct
    14821 cgcaggacca gaggaccagg acctcccagc ccagccgggc caaggcttcg gcattctccc
    14881 ggtcgcgttc catggttcgc ccgattttgg ccttccagaa ttccacgttg gtggacggaa
    14941 gcttggacct gggacaatcg ggatgtccgt gccagaaaca gccgtggacg aaaatggctt
    15001 tcttgcgccc ggggagcgcg atgtccggtt ttccgggcag cttcgaataa tgcagccggt
    15061 agcgaaagcc catggcatga aggacctttc tgacctgcaa ctcgggccgg gtgttgctcc
    15121 catgaattcg agacatgatc cagcttcgct gttgttccga gaacacatcc gccattgaat
    15181 tccttgtggg cgcaaaagac gtttctcccg accgcccctc tccgagaacc cccacccggc
    15241 ttgcccctct cccctcgagg gcccacgttc agtgtagggg catgcccggg agcagtcaac
    15301 gaagaaccgg gtcccgtgcc ggggacgccg gcgggctccc ttggatcaac ccggcccgcc
    15361 gtggagacga cgtctcgagg atcgcccgac ctcgatcggc ttccggtcgg ggtcgactcg
    15421 tggcaggcgc acgcggttgg tgtgggacat tttttagttt cgatgataag atcgtgaagc
    15481 cctcatgtta ggcaagggaa ttcaacgggt cccaacccat gacggtttcg taataagtcc
    15541 tgaagagtgc gtttcttcga ccctaaacga ttgaattcat taggtgccgt tccgagaaga
    15601 acgacttctt tcgggatccc gacgactcat gggttccgat tcctttgaga gtctttgccg
    15661 ggttttcccg gcagccccgg gtttcgggtc gaaatcgaca caaggccgaa gggggctcga
    15721 ggccccggca agtccggcag atgaaaccgt tttacgggaa cccgcaggca ggtggtgagc
    15781 atggacgaac ccggaatttc ccgcgagttg gccgcccggt acggccgcca ggccgttgaa
    15841 atcatcgaat ccggggaata cgtgtctcct tcgggtcgag tggttcaaat cgggcaccgg
    15901 gtaaaacgct ccgtcgaggg gacggtctcc taccctccgg atcgtccttt ccaagaatcg
    15961 gcccggggcc catttgctac cggaatcacc gtgcaaaaca ccacgaccct cgaggccgct
    16021 cgcaccttga ttgtcgcggg ggattttccg gcggtgctca actttgcttc ggcaacccac
    16081 ccggggggcg ggtttctctc gggggcccgg gcccaggagg agtccctggc ccgttcatcg
    16141 gccctttatg cctgtatccg gaacgatccc atgtacgtgt ttcaccggtc gcttcgggac
    16201 cctctttaca ccaattacgc catctattct cccgacgtgc cggtcttccg agccgacgac
    16261 ggcagtctcc tggaagggcc ctatacggtg gcgatcatca cctccccggc cgtgaacgcc
    16321 gtccggctgg acccggcaag aagaaacgaa atcggcccgg ccatgtggcg gcgtattctc
    16381 aaagtactgt ccatcggtct ccatcacggt cacgatgccc tggtcctggg ggcctggggc
    16441 tgtggggcct tcggcaacga cggccgtgaa atcgccgggt tgtttcacag ggctctcgag
    16501 gacaatttca agggggcctt ccgacacatt gtgtttgcgg tcctggatcg gtctcggggc
    16561 cggaaggtca tcggtccctt ccg
//
LOCUS       DAOOVC010000084        16583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2357_length_16583_cov_6.024262, whole genome shotgun sequence.
ACCESSION   DAOOVC010000084 DAOOVC010000000
VERSION     DAOOVC010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_16583_cov_6.024262"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLM79_11255"
     CDS             complement(<1..491)
                     /locus_tag="PLM79_11255"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOV86933.1"
                     /translation="MILSTHQPYFCPYPGFFFKAMESDIFVLLDSVQFPRGTTWISRN
                     RFKNDQGTLWLTIPVYKKGLGLQRIDRVRICYDGNWTGKHRESIRVAYGRSPYLEEHW
                     PLMDRVFSGNYETLVELNLEVIGYVAHLLGVRAEILRLSELEIHTRGTALILEICRTL
                     GADR"
     gene            complement(578..1360)
                     /locus_tag="PLM79_11260"
     CDS             complement(578..1360)
                     /locus_tag="PLM79_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV86934.1"
                     /translation="MGPDGGSRVDITEIEVRSALSVSRIPGVQYTINPYVGCEHGCRY
                     CYACFMSRYSRHHKDAPWGSFVEAKVNLVDLLARELPRKRKRSTVFLSSVCDPYQPAE
                     ERYRLTRGCLELLAAHGWGIEILTRSPLAGRDVDVLRGAAVVSVGFSIPTDNERVRQV
                     LEPNSPTIAQRVEALKEVRESGVSTWVFVAPMLPMDPLRLYAMIHPHVDSVLVDGLNY
                     SFRVKEIFHRNGWGYALSGQYAHRTGRKLLDLFGEKARQVCA"
     gene            complement(1758..2666)
                     /locus_tag="PLM79_11265"
     CDS             complement(1758..2666)
                     /locus_tag="PLM79_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV86935.1"
                     /translation="MRLLFSSDLHGNIPLYEELLRLAEEEGADAVILGGDLLPLKGPF
                     YQRIREQIEFVSSYLGPALEDFRSRHPHKGLYLLHGNTDWLPAVRLLEQFAQTGLFHF
                     LHGTIHSVGGSYRIAGYAHVPPTPFIMKEFERLDLRTSPLPVQQTTPCCSNGSRIVVV
                     NSDEHFRSRPSIEEELETLVKPPEGSGSLYVLHSPPQGTGLDVITSGEPVGSRAIRNF
                     IQRRQPRVTLHGHVHEAPAVSGVYASLIGKTLCINPGQTPTVLHAVFFDPDDLPGSLE
                     HTVYGRYSPNHADGGLDAASREQGEP"
     gene            3060..3737
                     /locus_tag="PLM79_11270"
     CDS             3060..3737
                     /locus_tag="PLM79_11270"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOV86936.1"
                     /translation="MAENLSFFKTLREGMVKEIEEDVKLTRSKIGKTALVPEVMRVMG
                     KVPRHEFVPLNLKSHAYENRPLPIGYGQTISQPYIVALMTDLLEPAPGQKVLEIGTGS
                     GYQAAVLAELVGRVYSIEIIPELAREAQARIKRLGYGNIEIRQGDGYYGWEDQAPFDG
                     IIVTAAGSHIPPPLVQQLKPGGKMVIPVGGSFMTQQLVVVEKLSDGKVRSRQVLPVAF
                     VPLTGGH"
     gene            3741..6203
                     /locus_tag="PLM79_11275"
     CDS             3741..6203
                     /locus_tag="PLM79_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOV86937.1"
                     /translation="MAVGLSVFCLSAASLAYELLLMRLLSIAQWNHFAFMIISLALLG
                     YGASGTFLVLCRRRIARRYAQSFVANAVLFSVSVQVCFSLARTIPLNPLEILWDSGQW
                     VFLFETYLLLSFPFFFAANGIGLTLTRFAGQVHRIYLFDLTGAGLGAGGVLLLLFYRT
                     PLEALKIVSALGFLSAVLAASSQRKELRHSRSVGVLLLAGGLFLAGSWAAGPGQVRIS
                     EYKGLSRSLRVPDARILREATSPLGWLAVVESPRIPFRYAPGSSLNCTREPPPQLGIF
                     IDGDSMTPVTRFDGNGETVSYLDCLPSVLPYFLLDRPEVLVLGAGGGGEVLSALVYGA
                     KSVDAVELDPNLVRLVRDPYGAFAGGIYSHPFVRVHIGDARGYLARSPRRYDLIHAAL
                     PDLSGAGASSAGGLSETYLLTVEAMGEYLEHLRPGGLLVLTRWLRIPPRETLKLVATV
                     IAAMERSGIRDPQNRLALVRSWNTATLLVKNGSFSREDLQRIETFCEERSFDVDYVPG
                     IRSGKGDRFNLLEEPYLVEGTLALLGRDRDDFIERYKFSVAPATDDRPYFFHFFRWKS
                     LPEFLSLSQKGGLPLLEWAYPLLILTLVQAATAAFLLVLLPVFFTGTSAMAPRGRMRT
                     GIYFSCLGLAFLFVEIALIRKFTLFLHHPLYSATVVLSGMLVFAGLGSGVSERAATRI
                     EKSLGPGSRGKVAAVVIFLIVSAALAELFFMPGVFRYFAGSSDFAKMAISLGVLAPLA
                     FLMGMPFPLGLALLREQTPERIPWAWGVNGCSSVVSAVLAAVLAIHAGFNGVMLAALL
                     LYGMGALAFWNFPAPATRGKEP"
     gene            6209..6613
                     /locus_tag="PLM79_11280"
     CDS             6209..6613
                     /locus_tag="PLM79_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86938.1"
                     /translation="MKKKVLFVCYGNSCRSILAEALARHHWKDSVEAASAGMDPLGYV
                     TPYTLAVLREIHVSTDGLESKAASAVDFSKIDLVVNLTNHPIRPYLPWKFPGKVIDWY
                     VTDPFGGTLDTFREARDAIEWLILKQLPRWLD"
     gene            complement(6623..6994)
                     /locus_tag="PLM79_11285"
     CDS             complement(6623..6994)
                     /locus_tag="PLM79_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86939.1"
                     /translation="MEAVKLDGPNGREFLFTLERRPSSLDDERGLRPFVYAVSPRGLE
                     ARWRGTALSRPLLDAVFLSPEGGILCALHRGDSFLNLDPGSLKTRTAVYRWNGFGFSG
                     IEDPGLQKACEERFEPLGPDR"
     gene            complement(6957..7520)
                     /locus_tag="PLM79_11290"
     CDS             complement(6957..7520)
                     /locus_tag="PLM79_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86940.1"
                     /translation="MVDFDVSPRGLETAGILKDKNGRFRLFLWNLEEEAVGRWMEVPG
                     SMELRSLACHPRGRNVFVSGGEGKAWGIWRVDLAREKPSFHPVLKSPREIRRIVPGPR
                     PFITRYDPKTGTPVPAYRIFYGSKNPAGTYSVRSITEARRTKERQRVFPGRVFHRGNP
                     GRKNRVEKEGGRDGVHGSGEARRPQRP"
     gene            8204..8569
                     /locus_tag="PLM79_11295"
     CDS             8204..8569
                     /locus_tag="PLM79_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HOV86941.1"
                     /translation="MGVVFKGIQSLGGNGVKGFHCIQCLSFISSSDLFLKLNGAQRHS
                     FVNPEGIYCEFYSFLSCPGAVPHGRDTEQDSWFPGYAWTPAFCVFCGNHLGWHYRAVS
                     VTSRPMEFWGILMDQVSVR"
     gene            8663..9322
                     /locus_tag="PLM79_11300"
     CDS             8663..9322
                     /locus_tag="PLM79_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV86942.1"
                     /translation="MKILNGLHGFLWTSYQANNCNAFLLDAGARILIDPGHLRLFDHV
                     KTGLRQLGLEVKDLDLVICTHGHPDHVESAKIFKKAGVQFAMHPRDWELGREMAVALG
                     ASVDPASLAPDFFLGEGDLDVKGLRLEVLHTPGHSPGSICLYWKEKKALFTGDLIFKD
                     GLGRTDLPGGNGEELKQSIRRVSNWDVEWLLPGHGDFIGGSTEVRRNFARVEEFWFVY
                     I"
     gene            complement(9416..9946)
                     /locus_tag="PLM79_11305"
     CDS             complement(9416..9946)
                     /locus_tag="PLM79_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86943.1"
                     /translation="MAEIKSTLELILEKTRHMTLTEEEKKEQARADLLISLRVLVQKY
                     QNGVLSLRDFASEYDRVRSSSREDAAGLLREELFRQLDPQKDNTSVLTLLGEICSVAV
                     SGITAVLDESREAVSRALEERTKHKLEELRNDRGISGSAVLPNLEADPRWKEDRERIR
                     DDYALRLARECARLTP"
     gene            complement(9984..10664)
                     /locus_tag="PLM79_11310"
     CDS             complement(9984..10664)
                     /locus_tag="PLM79_11310"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV86944.1"
                     /translation="MKQAPHAGRRSSRPVFSVCFLLLCVTVLALLSSCSYGDRKTTHH
                     AKPAPPPPEPPKEFQPYFDVNLGCPLPLVTNPILYVFKEKRRLLVVDRGILVREYRVG
                     LGSHPIGDKVMRGDGRTPEGEFFVCVKNPNSRFYKSLGLNYPTPRHAQEAMNWGKISQ
                     EQYRKIVDACSSQSVPPWNTPLGGAIMIHGGGTYADWTEGCVAVQNSTMDELFQIVSV
                     GTPVVIMP"
     gene            complement(10765..11589)
                     /locus_tag="PLM79_11315"
     CDS             complement(10765..11589)
                     /locus_tag="PLM79_11315"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV86945.1"
                     /translation="MGRIRYRSLWSLAVAWCLLYGCSKQYVDLRQIEDPPPSEGVYLP
                     PEEPRRKSRVKGPSILDKGVKRRQISEKDLAAACERNAGMDRTACLEILARLNSKDRS
                     YIPEDLKNKRPLLVPNSFEAYKDWSPLPKHIPELQKVPKLILVVKGIPFLGWYENGVL
                     AADSHICIGKQWGWTRTGMYSVLDKDINHFSQSYPNAYGEPAWMPFAIRVYERVWIHA
                     GDVVGGFCSHGCINVPINSAEKLFYWADLKTPVLILDSLDRLEAELKKIAGTGASG"
     gene            11928..12395
                     /gene="nrfH"
                     /locus_tag="PLM79_11320"
     CDS             11928..12395
                     /gene="nrfH"
                     /locus_tag="PLM79_11320"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOV86946.1"
                     /translation="MAVRNFLGRHRRFLQVFGTLAILGCVVGAFLSFGPPGLYAKSET
                     PEFCAGCHVMESQYENWFHHGAHRRVKCIDCHLPNDHTARHLARKGIDGMWDAYVFYS
                     GRVPDTITLSQSGAALVQENCFRCHGEMVSRINEDRNCWQCHRRVSHKISGAM"
     gene            12434..13921
                     /locus_tag="PLM79_11325"
     CDS             12434..13921
                     /locus_tag="PLM79_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOV86947.1"
                     /translation="MDRRGSFCGVVWPAILAACLGLSACAPEKPQPVRTGTIADGDYN
                     PANWGKVYPLEYESWAKTKDPRPAGKSKYKKGYDTDLVIYDKLSEFPYMPLLFNGWGF
                     GVEYNEPRGHHYMVIDQLEIDPSRLKAGGACLTCKTPFAPKLQQEMGKAYFADPYMEV
                     HAKIPKEFQQMGVACTDCHDNRTMSLQLSRWTLQKALADMGRDAAAVGRQESRMMVCA
                     QCHVTYVIPKDAEMKSTAVFFPWQGSSTGNISVENIIKVVRSDPSHLEWKQAVTGFKV
                     GFIRHPEYEFFSYKSVHYQAGVACADCHMPYIRVGANKISDHDVMSPLKNDLRGCQQC
                     HTESTEWLRNRIYTIQDRTVSLMNRAGYATAVAAKLFEMVHKAQGEGKKIDEALYERA
                     KDLYLDAFYRVIFIGAENSVGFHNPSEAGRICGDAVAMASKSESLLRQALAAAGVPVP
                     VDVRLEMAKYLQERGVKKLKFRPEFEFADPFNIQPRLTPAASLGK"
     gene            complement(14145..14942)
                     /gene="thiM"
                     /locus_tag="PLM79_11330"
     CDS             complement(14145..14942)
                     /gene="thiM"
                     /locus_tag="PLM79_11330"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOV86948.1"
                     /translation="MIQRICSGLEVLRARRPLVHNITNYVVMNFTANVLLALGASPVM
                     AHAVEEVEEMVALSSSLVVNIGTLSTPWIESMLEAARFASKRGIPLVLDPVGAGATQL
                     RTTTARTLLTEARVTVVRGNPSEILALAGEKAETKGVDSRADSRGARRAAAFLAKEYE
                     TVVAVTGATDFVTDGRKTVEVRNGHPLLQKVTGTGCAVSAVVGAFAAVEEDPLAAATG
                     ALVVFGVAGELAGVSNPRPGTYGVLLLDALDRVGASDLRERAGITES"
     gene            complement(14939..>16583)
                     /locus_tag="PLM79_11335"
     CDS             complement(14939..>16583)
                     /locus_tag="PLM79_11335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86949.1"
                     /translation="RDILVSAEILPTGGEDSVLWLIRDITEHRQAAKALREREKLFRT
                     LTENTPIGIALLSADRRFEYFNPHFTQLFGYTLMDLPDEDSWFAAVYPDPVYRAERLR
                     EWREHFHSRDPHGTARQRTLTLRCRDGSEKTVQSHSVSTEDGRILITFRDVTEQRRLE
                     AQLRQSQKMEAIGTLAGGIAHDFNNILAPILGYTEMALNEIPEGSRLHRNLSLVRAAS
                     HRAKDLVKQILAFGRQSEKELKPLRIHLVVKEAMVLLRATLPSTIEIRHEVVLDGEPE
                     MVMADPTQVHQVMINLCTNAAHAMRDKGGTLGVRLVRVTLASPLETHYTQIPPGSYVK
                     LTVSDTGHGIPPEDLHRVFEPYFTTKAPGEGTGMGLAVVYGIVSNHGGGITVESEPGR
                     GSAFHVFFPVVPPAKPKTEAPVPGTIPSGSGRILFVDDEPMLVGLGQYALRQFGYETV
                     ALSDSREALALFREDPHRFDLVVSDYTMPHMTGTELAVEMLRMRPDIPIVLCTGFSAS
                     ITEEQAKELGIRSFLLKPVSPVLLAQTISALLVEKERGK"
BASE COUNT         3074 a         4969 c         5201 g         3339 t
ORIGIN      
        1 cggtcggccc cgagagtccg gcagatctcg aggatgaggg ccgtgccccg ggtgtgaatc
       61 tccagttccg agagccgcaa aatctcggcc cggaccccca gaagatgcgc cacgtagccg
      121 atcacctcca ggttcaattc caccaacgtt tcgtagtttc cggagaatac ccggtccatg
      181 agcggccagt gctcttccag gtacggggat cggccgtagg caacccggat gctttcccgg
      241 tgtttcccgg tccaattccc gtcgtagcag atgcgcactc ggtcgatcct ctgaaggccc
      301 aaccccttct tgtagaccgg gatggtcagc cacagggttc cctggtcgtt cttgaaccgg
      361 tttcgagaaa tccacgtggt gccgcgggga aactgaacgg aatccagcag gacgaaaata
      421 tcggactcca ttgccttgaa aaagaatccc gggtacgggc aaaaataggg ctggtgggtc
      481 gaaaggatca tggttcgtct ccggcggcaa agcggcggac cgtctcaact acctcaagga
      541 tccccgtttt tgcggccgcg tgcggccagc ccatccctca tgcgcaaacc tgccgggcct
      601 tttcgccaaa aaggtccagg agctttcggc cggtccggtg ggcgtactgc ccgctaagcg
      661 cataccccca gccgttccgg tgaaagattt ccttcacccg gaaggaatag ttgagcccgt
      721 ccaccaacac ggaatccacg tggggatgaa tcatcgcgta taaccgcaac gggtccatgg
      781 gaagcatggg ggccacaaag acccaggtcg agacccccga ttcccggacc tccttgaggg
      841 cctccacccg ctgggcaatg gtgggggagt tgggttccaa cacttggcgg acccgttcgt
      901 tgtccgtggg gatggagaac ccgacggaaa cgacggcggc gcctcgcaga acgtccacgt
      961 cccttcctgc caggggagac cgggtaagga tttcgatgcc ccatccgtgg gccgccagga
     1021 gctcgagaca tccgcgggtc aaccggtacc gttcttcggc cggctgatac gggtcgcaga
     1081 cgctggagag aaaaacggtg ctcctcttcc gcttgcgcgg gagctcccgg gccaggagat
     1141 ccaccaagtt gacctttgcc tccacgaagc ttccccaggg tgcatccttg tggtgccggg
     1201 aataccgaga cataaagcag gcgtagcagt aacggcaacc gtgttcgcat cccacgtacg
     1261 ggttgatcgt gtactgcacg cccgggatcc tggaaacgct caaggcgctc cggacttcga
     1321 tttccgtaat gtccacccgg ctgccgccat ccggtcccat ggggttctcc actcctcttt
     1381 cctttgcacc cgagtggggc gattccgaaa gcatcggggc gtcatgcccg cgagggcgga
     1441 cccttaacca gcatgcatgc tctttccgga tttcccgcct gagcggaaat gacgaaacgg
     1501 tgtagaatgg cgaaacggga ttcataccgg gcaaatcgct cactccacct ctgtatttta
     1561 ccacgatcgg acgtgcagga taccaattcg gcacactccg cccaacccgc tatcgaaggt
     1621 gccgattgcc gtgcggcgct catgaatgga atcccacggt tgccggggaa gccataggcg
     1681 gccgaagcgg agcgtcgttt cgtcttcctc ccgacggaac gtcccggcga aaatccggcc
     1741 tcggtccgcg ggacggttta tggttcgccc tgctccctcg aggctgcatc caggcctcca
     1801 tcggcgtggt tcggggagta tcgtccgtag acggtgtgct cgagggaacc gggcaggtcg
     1861 tccgggtcga agaagacggc gtgcaaaacc gttggggttt gacccgggtt gatgcaaaga
     1921 gtcttcccga tcaaactcgc gtagactccg ctcacggccg gggcctcgtg cacatgtccg
     1981 tggagggtca cccggggttg gcggcgttgg atgaagttcc ggatggcgcg actccccaca
     2041 ggttctccgc tggtgatgac gtccagcccg gtcccctggg gaggggaatg gagaacatag
     2101 aggctccctg aaccttccgg cggtttcacc agggtttcga gttcttcttc aatggacggg
     2161 cgggaacgga aatgttcgtc ggaattcacc accacgatcc tcgacccatt gctgcagcag
     2221 ggggttgtct gctgcacggg aaggggactg gttcgcaggt ccaatcgttc gaattccttc
     2281 atgataaaag gcgttggagg aacatgggca tagcccgcaa tccggtaaga cccgcccacg
     2341 gagtggatcg tcccgtggag aaagtgaaag agtcccgttt gggcgaactg ctccagcagg
     2401 cggacggcag gcaaccagtc ggtgttgccg tggagcaggt aaaggccctt gtgcggatgg
     2461 cggcttcgga agtcctcgag ggcgggtccc aggtaggagg aaacaaactc gatttgttcc
     2521 cggattctct ggtaaaaagg tcccttgagg ggaagaaggt ccccgcccag gatgaccgcg
     2581 tccgcccctt cttcttccgc caaccgcaga agttcttcgt acagggggat gtttccgtga
     2641 agatctgagc tgaacaagag tctcatgagc cgattttcac ctcggggaag gcccttccgt
     2701 ctgtcccggg aaaacccggc acggggaacg cccccttttt cgaggacaag gagaccggaa
     2761 tgatttcgat ccttctttca tttccgcccg gctggaatgg ggccccggca cgatccggcc
     2821 ccggagtccg ccgcgacgga tacccgggct ttccccgcgc gattccaggc acggcatccg
     2881 gggacggccc ccgcgagtcg gggtcgcgtg attcctcggc tgaatccggg gccctcgcgg
     2941 acccgacccc gaaatccgtg cacccgggcc cggtggttcc cgtcatccgg agatcggacc
     3001 ccctccgcgt cgccgcggtg cttcttttcc ttgtctgcgt gtggggggcc cgacccgtca
     3061 tggcggaaaa tctctccttt ttcaagaccc tccgggaagg aatggtaaag gaaattgaag
     3121 aggatgtgaa gctgacccgc tcgaaaatcg gcaaaacggc cctggtcccc gaagtcatga
     3181 gggtcatggg aaaggttccc cgccatgagt tcgtgccgct gaacctgaaa tcccacgcct
     3241 atgaaaaccg tccccttccc atcgggtacg ggcagaccat atcccagccc tacatcgtgg
     3301 ccctcatgac ggacctcctg gaaccggcgc cggggcagaa ggtgctcgag atcggtaccg
     3361 ggtccggtta ccaggcggcg gtcctggcgg aactggtcgg ccgggtgtac tccatcgaga
     3421 tcattcccga gcttgcccgg gaggcccagg cccgcatcaa gaggctgggc tacggcaata
     3481 tcgagattcg acagggggat ggatattacg gatgggaaga tcaggctccc tttgacggga
     3541 tcatcgtcac cgccgccgga agccacatcc cgcctcccct cgttcagcaa ctgaaaccgg
     3601 gcgggaaaat ggtcattccc gtgggaggtt ctttcatgac ccagcaactg gtggtggtgg
     3661 aaaaactatc cgacggcaag gtgcgctcga ggcaggtcct gcccgtggcc ttcgtcccgc
     3721 tcacaggggg ccattgaccc atggcggtgg gcctgtccgt cttttgcctc tcggccgcct
     3781 ccctggccta tgaactgctg ctcatgaggc ttctcagcat tgcccagtgg aatcacttcg
     3841 cgttcatgat catcagcctg gcactgctgg gatatggagc aagcgggacc ttcctggtcc
     3901 tctgtcggcg caggatcgcc cggcgatatg cccaaagctt tgttgccaat gcagttcttt
     3961 tcagcgtttc ggtgcaggtc tgtttttccc tggcccggac aatccccttg aacccgctcg
     4021 agatcctctg ggactccggg cagtgggtgt ttcttttcga gacctacctt ctcctttcct
     4081 ttcccttctt ttttgcggcc aacgggattg gcttgaccct cacccggttc gcggggcagg
     4141 ttcaccggat ctatttattc gatctcacgg gggcgggact gggcgccggg ggcgtcctat
     4201 tgctgctgtt ctatcggacg cccctcgagg ccctgaagat cgtgagcgcc ctgggtttcc
     4261 tgtcggcggt tttggcggcg tcgagccaga gaaaggagct acgccactct cgatccgtgg
     4321 gggtcttgct cctggcgggg gggctcttcc ttgcggggag ctgggcggcg ggacccgggc
     4381 aggtgcgcat ttcggaatac aagggactca gccgatccct tcgagtcccg gatgcaagga
     4441 ttctgcggga agcaaccagc ccgttgggct ggctggccgt ggtcgaaagc ccccggattc
     4501 cgtttcgcta cgccccgggc tcgagcctca actgcacccg ggaacctccc ccccagctgg
     4561 gcatcttcat cgacggggat tccatgactc ccgtcacgcg cttcgacggc aatggggaaa
     4621 cggtgtctta tctcgattgc ctcccgtcgg ttctgcccta tttcctgttg gatcgccccg
     4681 aggtcctggt gctgggggcg ggaggaggcg gggaggtcct ttcggccctg gtgtacgggg
     4741 ccaaatccgt ggacgccgtc gaactggacc ccaacctcgt ccgcctcgtc cgggatcctt
     4801 acggcgcctt tgccgggggc atctacagcc atccgttcgt tcgcgttcat atcggggacg
     4861 ctcgcggcta cctcgctcga tccccccggc gctacgatct cattcatgcg gctcttccgg
     4921 acctgtccgg cgcaggggct tcgagcgccg ggggattgag cgaaacctac ctccttacgg
     4981 tggaagccat gggcgaatac ctggaacacc tgcggccggg gggccttctt gtgttgaccc
     5041 ggtggttgcg gattccgccc cgggaaaccc tgaagctggt tgccaccgtg atcgccgcca
     5101 tggaacggtc gggaatccgg gacccccaaa accgcctggc cctggttcgc agctggaata
     5161 ccgccaccct gctggtgaaa aacgggtcct tttctcggga ggatcttcaa aggatcgaaa
     5221 ccttctgcga ggagaggtcc ttcgacgtgg actacgttcc gggaattcgt tcggggaagg
     5281 gggatcgctt caacctgctg gaagagccgt acctggtcga ggggaccctg gccctgctgg
     5341 ggagggatcg agatgatttc atcgagcgct acaagttttc cgtggctccc gccacggacg
     5401 atcgccccta tttcttccat ttctttcggt ggaagagcct ccccgaattc ctttcgctga
     5461 gccagaaggg tgggctgcct ctcctcgagt gggcctatcc cctcttgatc ctgaccctgg
     5521 tgcaggccgc gacggccgcc ttcttgctgg ttctgctccc ggtcttcttc acagggacca
     5581 gcgcaatggc gcctcgaggc cgaatgcgca ccgggatcta tttctcgtgc ctgggactgg
     5641 cgtttctctt cgtggaaatc gctctcattc ggaagttcac tcttttcctg catcatccgc
     5701 tgtattcggc cacggtggtg ctgagcggaa tgctggtttt tgcaggcctg ggaagcgggg
     5761 tctcggaaag ggcggcaacc cggatcgaga agagcctggg gccgggttcc cggggaaagg
     5821 tcgcggcggt ggtgatcttc ctgatcgtga gtgcggccct ggcggaattg tttttcatgc
     5881 ccggggtttt ccgctatttt gcggggtcga gcgatttcgc aaaaatggcg atctccctgg
     5941 gggttctcgc tcccctggcg tttctcatgg ggatgccctt tcccctgggc ctggccctcc
     6001 tcagggaaca aaccccggag aggatcccct gggcctgggg agtcaacggc tgcagctccg
     6061 tcgtcagcgc ggtcctggcc gccgttttgg caattcacgc gggattcaac ggcgtgatgc
     6121 tggcggccct tcttctttac ggcatgggag ccttggcctt ctggaatttc ccggccccgg
     6181 caacccgggg aaaggagccg tgaacccaat gaaaaagaag gtgctcttcg tttgttacgg
     6241 caattcctgt cgcagtattt tggccgaagc cctggcccgg caccactgga aagattcggt
     6301 ggaagcggcc tcggcgggca tggacccctt gggctacgtg accccctaca ccctggcggt
     6361 cctgcgggaa atccacgttt ctacggacgg gctcgagtcc aaggccgcct cggccgtcga
     6421 tttctcgaag atcgacctgg tggtcaatct caccaaccac cccatccggc cctaccttcc
     6481 gtggaaattc ccgggaaagg tgatcgactg gtacgtgacg gaccccttcg gaggaaccct
     6541 cgataccttt cgagaggcac gggacgccat cgagtggctg atcctgaaac agctccccag
     6601 gtggctggac tgacgggccc cgtcaacgat ccgggccgag gggttcgaac cgctcctcgc
     6661 aggccttttg cagtcccggg tcctcgatcc ccgaaaatcc gaaaccgttc cagcggtaaa
     6721 cggccgtgcg ggtttttagg gaaccgggat cgaggttgag gaaggaatcg ccccggtgga
     6781 gggcgcacag gattccgccc tcgggggaaa ggaagaccgc gtccaatagg ggccgggaaa
     6841 gagcggttcc ccgccatcgg gcctcgagac cccgggggga cacggcgtac acaaaagggc
     6901 gcaggccccg ttcgtcgtcc agggacgacg ggcgcctctc gagggtgaaa agaaattcac
     6961 ggccgttggg gccgtcgagc ttcaccgctt ccatggacac catcccgtcc cccttccttc
     7021 tccacacggt ttttccttcc cgggttccct cgatgaaaga ctctcccagg gaaaaccctt
     7081 tgccgctcct tggtccgacg ggcctcggtg atcgagcgaa cggaataggt gcctgcgggg
     7141 tttttcgatc cgtagaagat tcgataagcc ggaaccggcg ttccggtctt tgggtcgtaa
     7201 cgggtaatga aaggacgggg gcccggaacg atcctccgga tctcgcgggg ggacttgagc
     7261 acagggtgaa acgagggctt ttcccgggca aggtccacgc gccagatgcc ccaggctttt
     7321 ccctccccgc ccgacacgaa gacgtttcgc ccccgggggt gacaggccag ggatcggagc
     7381 tccatggacc cgggcacctc catccaccgt ccgacggcct cttcctcgag gttccacagg
     7441 aacaaccgga accggccgtt cttgtccttg aggatccctg ccgtttccaa tccccgggga
     7501 gaaacatcga aatccaccac ctgagcggca tcggggaggt tcagggttcg gaccggttgc
     7561 gggcgagggg ctcaccgaaa gctcctcttc gaaaaaaacg gtttccgacc ggggggccgt
     7621 cggcagggcg aagaggagca gaacaacgag tacgcagacg agaatttttt ccatggtcgt
     7681 tctcctgttc catgcgatgg gggggcggga atacccggca gagagtagca tctccttcgg
     7741 ggaagatcgc ggggtcaggt ctccgtatgg ctgacctcag gggtcgtgtc cgcgtatggg
     7801 gtgtcccggc cgtggagaga gtgtaggggc gaccggccgg tggcccccaa ccaggcgtgt
     7861 ggggtgcaat ccccctcttg ttcacgggac ggtcctgcga ccccatgggt ggacttcctg
     7921 ttggggcagg ggtatcggcc ttggggcctc gacctccggc taatggctgc aaaccctccg
     7981 ggttcgcccc aaggcggcga tctatccccg cctgatcgca gaggtgggta acgggcggag
     8041 gcctcgacgg accgttccgc cgggtttgac ttccgtcggt ccggggttgt atgcttccag
     8101 aacggcaggg tgagggagcg gagaagaaaa ccgggggcgg ttggttccgg attcgggttt
     8161 ccaccaacga gggcaatgtt tcggaaacgg tcgagagagg atcatggggg ttgttttcaa
     8221 gggaattcag agtctcgggg gaaacggggt caaaggattt cactgcatcc agtgtctcag
     8281 cttcatcagt tcttcggatc tttttttgaa attgaacgga gctcaacggc attccttcgt
     8341 gaaccccgag ggcatctatt gcgagttcta ctcctttctt tcctgtcccg gggcggtgcc
     8401 gcacggccgg gacacggagc aggattcgtg gtttcccggc tacgcgtgga ctccggcttt
     8461 ctgcgtcttt tgcggcaatc atctgggatg gcattaccgg gcggtttccg tcacgtcccg
     8521 gcccatggag ttctggggaa ttctcatgga ccaggtgagt gttcgctagg ggacccgtaa
     8581 ggcccggctc gcccggcgcc ggcatcgaat ccccggacgg acgggcccgg caaaacggcg
     8641 atcattggga gaggatgggg aaatgaaaat tctgaacggc cttcacggct tcctctggac
     8701 ttcgtaccag gccaacaact gcaatgcctt cctcctggat gcaggggcgc ggatcctgat
     8761 cgacccaggg cacctgcgac tcttcgacca cgtgaaaacg ggccttcggc agttggggct
     8821 cgaggtgaaa gatctcgacc tggtgatctg cacccacggg catcccgatc acgtggaatc
     8881 cgccaagatt ttcaagaagg ccggggtgca atttgccatg cacccccggg actgggaact
     8941 gggccgggaa atggcggttg ccctgggggc ttcggtggat ccggcttccc ttgctccgga
     9001 ctttttcctc ggggaagggg acctcgacgt gaaggggctg cgcctcgagg tcctccatac
     9061 ccccgggcat tctcccggct ccatctgtct ttactggaag gaaaagaagg cgctctttac
     9121 cggggacctg atcttcaagg acgggctggg tcgaacggac cttcccgggg gcaacggcga
     9181 agagctcaaa cagagcatcc ggagggtctc gaactgggac gtcgaatggc tcttgccagg
     9241 ccatggggat ttcatcggcg ggtcgacgga ggtccggcgg aactttgcgc gagtggaaga
     9301 gttctggttt gtctacattt agcgtggaat ctgccgggag gtcccggcgc gaaatccgct
     9361 cgacgattgt cccctcgatg ggccgagggg ggcggccgca tccccccggt ccggctcatg
     9421 gggtcagccg ggcgcattcc cgggcgagcc tgagggcgta gtcgtcccgg attcgctccc
     9481 ggtcttcttt ccatcgaggg tccgcttcca ggttgggcag gacggcgctg ccggagatgc
     9541 cgcgatcgtt gcggagttcc tcgagcttgt gcttggtgcg ttcctccaag gcccgggaca
     9601 cggcttcccg ggattcgtcg agaaccgccg tgatgcccga cacggcaacg gagcagatct
     9661 ccccgaggag ggtcaggacg gaggtgttgt ccttttgggg atccagctga cgaaagagtt
     9721 cttctcgaag caaccctgcc gcatcttccc gggaagagga gcgaacccgg tcatattcgg
     9781 acgcaaagtc ccggagactc aaaacgccgt tttggtactt ttggacgagc actctcaagg
     9841 agatcagaag gtcggctcgg gcctgctcct tcttctcctc ttccgtaagg gtcatgtgac
     9901 gggttttctc caggatcaat tcgagggtac ttttgatctc ggccatgggt aatcctcctt
     9961 gaaaccttcc tgcccgggag aacctagggc atgatcacca ccggcgtccc cacggatacg
    10021 atttggaaca gttcatccat ggtggagttc tggaccgcaa cacaaccttc ggtccagtcg
    10081 gcgtaggttc ctcccccgtg aatcatgatg gcgcccccca ggggggtgtt ccaaggagga
    10141 accgactggg aactgcaggc atccacaatt ttgcggtact gctcctggga aatcttcccc
    10201 cagttcatgg cttcctgggc gtgccggggc gtggggtagt ttaggcccag ggacttataa
    10261 aacctgctgt tcggattctt gacgcacacg aagaattccc cctcgggggt tcgcccgtcg
    10321 ccccgcatca ccttgtctcc gatgggatgg gaacccagtc ccacgcgata ttcccgtacc
    10381 aggatgcccc ggtcgaccac cagcagcctc cgcttttcct tgaacacgta gaggatgggg
    10441 ttggtcacca ggggtagagg gcagcccagg ttcacgtcga aatagggctg gaattccttg
    10501 gggggttccg gcgggggtgg agcgggcttg gcgtggtggg tggtctttcg gtccccataa
    10561 gaacaggaag aaagaagcgc gaggaccgtc acgcaaagca gaaggaagca gacggaaaaa
    10621 acgggacgcg acgatcgacg gcctgcatgc ggtgcttgct tcatggtgat tcgactccgg
    10681 tgactccaga aagatgtttg caaatcccag ggctccggtc aaggtcgcca acgacccgag
    10741 cccgtatcct ccccccccgg ggggttaccc ggatgctccg gtgcccgcaa tctttttcaa
    10801 ttccgcctcg aggcggtcca gggaatccag gatcagaaca ggggttttca gatcggccca
    10861 gtaaaagagt ttctccgccg agttgatggg aacgttgatg cagccgtgag aacagaagcc
    10921 gccaacgacg tcccccgcat gaatccacac ccgttcgtac acgcgaatgg caaagggcat
    10981 ccaggccggt tctccgtaag cattgggata gctctgggag aagtggttga tgtctttgtc
    11041 cagcacggaa tacatgccgg tcctggtcca tccccattgc tttccgatgc agatgtgact
    11101 gtctgccgcc aggaccccgt tttcatacca cccgaggaag gggatccctt tcaccacaag
    11161 gatgagcttg ggaacttttt gaagctccgg gatgtgcttg ggcaggggac tccaatcctt
    11221 gtaggcctcg aaactgttgg gtacgagaag gggacgcttg ttcttgaggt cctcgggaat
    11281 ataggaacgg tccttggaat tgagccgggc gaggatttcc aggcaagcgg ttcggtccat
    11341 accggcattt ctttcacacg cggccgccag gtccttctcc gagatctggc gccgtttcac
    11401 ccccttgtcc aggatcgagg ggcccttgac ccgggacttt cggcgcggtt cttccggggg
    11461 gaggtagacc ccctcggacg ggggcggatc ctcgatttgc cgcagatcca cgtactgctt
    11521 ggaacaaccg tagagcaaac accaggcaac ggcgagactc cagagagaac gataccggat
    11581 tcttcccaaa gggattcctt tccttgaagg tccccgggct tttccccggg gccgattcat
    11641 agggatcttt acaacggttc gaatcgtccc gcaagtttct tcaaaacgct tctcgttcaa
    11701 atcaattgac ttaagtcaaa agaatatcta cagggatccg gtatgctcgc tttgcagctt
    11761 caatgacccc atcgctcatg cgcggacttc atttccagcc ggcccctgtc gagaaccacg
    11821 ccttcacgga cgcgacttgc cctgatttgt gcagtgcacg tcccgtttgt gggaagagac
    11881 ggcgtgacgc ccttttcaat ccggatttag aagggagaag aaggccaatg gcggttcgaa
    11941 atttcctggg aagacacagg cgattcctcc aggtgttcgg cactctcgcc attctgggat
    12001 gcgtggtggg tgccttcctg agcttcgggc ctccgggctt gtacgccaag tcggagaccc
    12061 cggagttctg cgccgggtgc cacgtcatgg aatcccagta cgaaaactgg ttccaccacg
    12121 gagcccaccg gcgggtaaaa tgcatcgatt gtcatctgcc caacgaccat acggcgcgac
    12181 acctggcccg gaagggcatc gacgggatgt gggatgccta tgtcttttac tcgggtcgag
    12241 tccccgacac cattaccctg tcccagtccg gggccgccct cgtccaggag aactgctttc
    12301 gatgccacgg agaaatggtg tcgcggatca acgaagaccg gaactgctgg cagtgtcacc
    12361 gcagggtgtc ccacaagatc agtggagcca tgtaatggga agggctgaga gctcaacgaa
    12421 aaggaggtcg agtatggatc gtcgaggatc cttttgtggc gtggtctggc cggctattct
    12481 cgcggcctgc cttggattgt ccgcttgtgc tccggaaaag ccgcagccgg tgagaaccgg
    12541 gaccatcgcc gacggagact acaatcccgc caactggggg aaagtctacc ccctcgagta
    12601 cgagtcctgg gcaaagacca aggatccccg gcccgcgggc aagagcaagt acaagaaggg
    12661 atacgacacc gatttggtca tctacgacaa gttgagcgaa tttccctaca tgccgctgct
    12721 gttcaacggt tgggggttcg gcgtcgagta caacgagccc cgaggacacc actacatggt
    12781 gatcgatcag ctcgagatcg acccgtcccg tttgaaggcg ggaggcgcct gtcttacctg
    12841 caagactccc ttcgccccca agctgcagca ggaaatgggg aaggcctatt ttgcggatcc
    12901 ctacatggag gtgcatgcca agattcccaa ggagtttcag cagatggggg tggcctgcac
    12961 ggattgccac gacaaccgga ccatgtctct ccaattgtcc cggtggaccc tacagaaggc
    13021 cctggccgac atgggaagag acgcggccgc cgtcggccgc caggaaagcc gcatgatggt
    13081 gtgcgcccag tgccacgtga cctacgtcat ccccaaggac gccgagatga aatccacggc
    13141 cgtcttcttc ccctggcagg ggagcagtac gggcaacatc tcggtggaaa acatcatcaa
    13201 ggtggtgagg agcgatccgt cccacctcga gtggaaacag gcggtgaccg ggttcaaggt
    13261 gggattcatc cggcatccgg aatacgagtt cttcagctac aagagtgttc actaccaggc
    13321 gggagtcgcc tgtgccgact gccacatgcc ctacattcgg gtgggggcca acaagatttc
    13381 ggaccacgac gtcatgagtc ccctcaagaa cgatctgcgg ggatgtcagc agtgtcacac
    13441 ggaatcgacg gaatggctga gaaaccggat ctacaccatc caggaccgca ccgtgtccct
    13501 gatgaatcgt gcgggctatg ccacggcggt ggcggcaaag cttttcgaaa tggtccacaa
    13561 ggcccagggc gaaggaaaaa agattgacga agccctctac gaacgagcca aggacctgta
    13621 cctggatgcc ttctaccggg tgatcttcat cggtgcggaa aattccgtgg gatttcataa
    13681 tcccagcgag gcggggcgaa tctgcggaga cgcggtggcc atggcgtcca agagcgagtc
    13741 ccttcttcgc caggccctgg cggcggccgg ggttcccgtt cctgtggacg tccggctcga
    13801 gatggccaaa tacctccagg aacggggagt caagaagctc aagtttcgcc ccgagttcga
    13861 attcgccgac ccgttcaaca tccagccgcg gttgactccg gcggccagcc tgggcaagta
    13921 accgctcgag gatctcaccc ggtccaaacg ccagcgccgc ctgtcacgac gacccaaggg
    13981 ggattgacaa ggctccgggg gttgagggtg aaaagggtaa tgcaccgcct cccacggcgg
    14041 tgtccaaaat cgagggccta tccggggaag ggggactccc ccgttcacgg atgggctctt
    14101 ttttgtgaag ggccttcccc cgatgcgcgc atcgctcgcc cttgtcagga ctcggtaatc
    14161 ccggctcttt cccggaggtc cgatgcgccg accctgtcca gggcgtccag gagcagcacc
    14221 ccgtaggttc ccggacgagg gttggaaacc ccggccaact ccccggcaac tccgaaaacc
    14281 accagcgctc ccgtcgccgc cgccaggggg tcctcttcca cggccgcaaa ggccccgaca
    14341 accgccgaaa cggcgcatcc cgttcccgtt accttttgca gcaggggatg accgtttcgc
    14401 acttccacgg tctttcgccc gtcggtgacg aagtccgtgg ctccggtgac ggcaaccacc
    14461 gtttcatatt ctttggccag gaatgcggcc gcccggcgtg ctccccggga gtccgcccgg
    14521 gaatccactc ccttggtttc cgccttctcc cccgccaggg caagaatttc cgaaggattt
    14581 ccccgcacaa cggtgacccg ggcctcggtg aggagggttc gagccgtcgt ggtgcgaagc
    14641 tgggtcgccc cggctcccac cgggtccagc accaggggga tgccccgctt cgacgcaaac
    14701 cttgcggcct cgagcatgga ctcgatccag ggggtggaaa gggtgccgat attcacgacc
    14761 aggctcgagg aaagggcgac catttcttcc acttcctcca ccgcgtgggc catgaccggg
    14821 gaagccccca gggccagcaa aacattggcg gtgaaattca tgaccacgta attggtgatg
    14881 ttgtggacca gcggccgccg ggctcggaga acctcgaggc cggaacagat tcgttggatc
    14941 attttcccct ttccttttcc accaggaggg cggagatggt ttgagccagc aggacgggac
    15001 tgaccggttt caaaagaaag gaccggattc ccagttcctt ggcctgttct tcggtaatgg
    15061 atgcgctgaa gccggtgcac agcacgatgg ggatgtcggg gcgcatgcgc agcatctcca
    15121 cggccagttc ggtgccggtc atgtggggca tggtatagtc gctgacgacc aggtcgaaac
    15181 ggtgtgggtc ctcccggaaa agggccagag cttcccggga gtcggacagg gcgacggtct
    15241 cgtatccgaa ttggcgaagc gcgtactgac ccagtccaac gagcatcggt tcgtcgtcca
    15301 cgaacaggat tctgcccgag cccgacggaa tcgttcccgg gacgggcgct tcggttttgg
    15361 gctttgccgg cggcaccacg ggaaaaaaga cgtgaaaggc gctcccccgg ccgggttcgc
    15421 tctccaccgt gatcccccct ccatggttgc tgacgatgcc gtacaccacc gccagtccca
    15481 ttcccgttcc ttcccccggg gccttggtgg tgaaataggg ctcgaaaacc ctgtgcaggt
    15541 cctccggggg aatgccgtgg ccggtgtcgg atacggtgag cttcacgtag cttcccgggg
    15601 ggatttgggt gtaatgggtc tcgaggggtg aagccagggt aacccggacc aggcgcaccc
    15661 ccagggtccc gcccttgtcc cgcatggcgt gggcggcgtt ggtgcacaag ttgatcatca
    15721 cctggtggac ctgggtgggg tccgccatga ccatctcggg ttcgccgtcc aggacgacct
    15781 cgtggcgaat ctcgatggtg gaaggaaggg tcgcccggag aagcaccatg gcttccttga
    15841 ccaccaggtg aattcgcagg ggtttgagct ccttctcgga ctgccggccg aaggcgagga
    15901 tctgcttgac caggtccttg gctcggtggg acgcggcacg aaccaggctt agattgcggt
    15961 gcagtcggct gccttccggg atttcgttga gggccatctc ggtgtagccc aggatcggcg
    16021 ccaaaatgtt attgaaatca tgagcaattc ccccggccag ggtgccgatg gcctccattt
    16081 tctgggactg gcgcagttgt gcctccaatc gccgctgttc ggtgacgtct cggaaggtga
    16141 tgagaattcg gccgtcttcc gtggacacgg agtgggactg gaccgttttt tcggagccgt
    16201 ccctgcaccg cagggtcagg gttcgttgtc gggctgtccc gtgggggtcc cggctgtgga
    16261 aatgctctct ccattcccgg agacgttcag cccggtaaac ggggtccggg tacacggcgg
    16321 caaaccagga gtcttcgtcg ggcaggtcca tgagggtata gccaaagagc tgggtgaagt
    16381 gcggattgaa gtattcgaac cggcggtccg cactgaggag ggcgatgccg atgggagtgt
    16441 tctcggtcag agtccggaag agcttttccc tctcccgcaa ggcctttgcc gcttgcctgt
    16501 gttcggtgat gtctcgaatc agccaaagca ccgagtcctc cccacccgtg ggaaggattt
    16561 ccgcggaaac caggatgtcc cgg
//
LOCUS       DAOOVC010000085        16307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2405_length_16307_cov_6.021905, whole genome shotgun sequence.
ACCESSION   DAOOVC010000085 DAOOVC010000000
VERSION     DAOOVC010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16307
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2405_length_16307_cov_6.021905"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLM79_11340"
     CDS             <1..234
                     /locus_tag="PLM79_11340"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86950.1"
                     /translation="HGLGQGGMPMERSGRILGAHILGPRASDLIAECVLAMQVGGSAE
                     DLAGTIHGHPTLSEAVHEAAAAVRRCSIYAPRP"
     gene            454..1656
                     /locus_tag="PLM79_11345"
     CDS             454..1656
                     /locus_tag="PLM79_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86951.1"
                     /translation="MNNKLRIVVTGLAATYPFGGVFWDYLQYVLGFRRLGHEVLYLED
                     TGKWCYDPSAGTFVEDGRNNAAFLSAELHRLDPSLEGHWFYRDSTGKTYGRQWRDVVS
                     FCRSADLFLHVSASCWMREEYFAASRVAFIDSDPMYTQASIPGYLDGSLDEEARSRVD
                     TLLQHDVFFTFGENVGASDCRIPTELFRWIPTRQPVVLDCFRDHRIPVSARRRVLTTV
                     ASWEPSEKGPVVRGVAYGGKSIEFNRFMDLPSRSVLPLELAMSGRAPRDRLTAAGWGV
                     RDGYEVSANPWIYRDYLADSLGEWSVAKNAYVLSRSGWFSCRTACYLALSVPAVVQDT
                     GFDRFIPTGEGLFAFNTPEEAVEAISRIESEPERHAAAAAAVAAEYFDSDMVLRQLIE
                     DAFSSSSN"
     gene            1767..2765
                     /locus_tag="PLM79_11350"
     CDS             1767..2765
                     /locus_tag="PLM79_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOV86952.1"
                     /translation="MYSPIVFRCLLAVLGVVLWAAATSPCRAGMVSLTFDDGLSSVYS
                     EAYPVLKRHNLKATVGMVVNRITSGNDDYISLQEAHELQDAGWEIASHGLTHQRPIDI
                     PQYFEDEVVEGWEPDGTNAEVYEAKYDYHSVAGLLENGKPLKEFTRLEDVRRAKGSFY
                     FDRLIGALHVHPFNPMNAEREDIRSYSYQRELCESKIALEKLGFKISTYITPFNYWTE
                     EMASLSKLYYSQVANGKNMANRKETCDLNWLYRFNVLSMGKTQDAVRLIQKHAVEQDE
                     WVIFCLHGIEDEMGWEPWSADNLEELAAWLQKNQIRVVTVSEGAALYSSVPQARRR"
     gene            2773..3942
                     /locus_tag="PLM79_11355"
     CDS             2773..3942
                     /locus_tag="PLM79_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV86953.1"
                     /translation="MKGRPLKIVVAGFAIGFPMGGQLWMMMHYVLGLKQLGHEVLFLE
                     DTSDWSYAYDPVRRSYDVDSSYGRRVLEDFFRRNGLEGQWVYNSALENRIYGRTRQEL
                     DRFCSEADLWLSLSGTNPLRENYMRCRVKAVIDTDPVFTQLKIRDDEITRDYFKAHDV
                     CFTWGYNLPSLKTRVPLSGIDWKPTLPPILLEKWAPVSPPGRSYNTIGFWDTKGRDIV
                     LDGEVFSWRKRPKYERLIDLPGQLPGLPLEIAFMGIEDDGPRYADHGWILRDPVAVSA
                     DPYNYMDYIKNSRAEFTVAKDQNVKLKSGWFSDRSASYLAAGRPVVTEDTGFSAYLPV
                     GEGIFPYETREEAIESIRTIERDPVHHSRAALHLAEEHFDARKVLTEILREVGLA"
     gene            3993..4892
                     /locus_tag="PLM79_11360"
     CDS             3993..4892
                     /locus_tag="PLM79_11360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOV86954.1"
                     /translation="MRGAALDPGPTQRLAWVDVTKGISILWIVFFHYFKTYAKGRYPW
                     PLDPGFLSESLARCEFASLLSNLVCLADSLFAAVSHLGFHAVGVFLLMSGFGLTFSLA
                     KTGNPAEGWAGWYRSRLLRLFPMYWIAHFIYVVSPLVARPEPIDSRFFLSFLGNRVYP
                     IETLFYYMNPAWWFVGLLLQLYLVFPLLFRMLQKLGIGRFILVSTLITLLSRTLLLFV
                     LQANGDYLQGGFFGSRLIEFAAGMALGYAYRKQHDLADSSSSTGKPWRQGPASMPWES
                     RATSDYGLTCLRTLSSAWVFPSC"
     gene            4943..5134
                     /locus_tag="PLM79_11365"
     CDS             4943..5134
                     /locus_tag="PLM79_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86955.1"
                     /translation="MARVGVYSYGLYLLHQPYLITLGENTRQLAMIPSTLLALAVITA
                     IALPCMVLEKSVNRLMRHW"
     gene            complement(5150..6946)
                     /locus_tag="PLM79_11370"
     CDS             complement(5150..6946)
                     /locus_tag="PLM79_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV86956.1"
                     /translation="MQSSFPTIRKVFPYLWPYRVPFLAALGQVFLISACELLKPWPLK
                     IIIDNVLSNQPLDWIWLGGLQQDTLLVVACIGMVVIYVLLGSILLLNNYTTIKIGQRM
                     VNDLRGDLYNHLQRLSLSFHSRRQVGDLLYRVAADTYSIQSLTMNGVFPIVSAVVLLV
                     GMFVIMIRMDWVLTLLALGVCPLLLLSISVFGKTLTSAATTARQQESEVYSLVQRAMS
                     AMRVIQAFTKEEEEHRRFMRASEQSLGSSLRLYVLQTFYSGLVNVIMAVGTAVVVWVG
                     ARHVMSGTLSVGEIVVFTSYLASLYGPINTISQTWGMLQGSKVGVQRVFEILEVERDL
                     PEGTRTFSEDGARGEIEVQGVSFHYVPEQPVLRKIDLKVRKGQKVAIVGPTGVGKSTL
                     VSLIPRFYDPQSGRILIDGTDVKEFQLKSLRSQISMVLQPPLVFPMTIRENIAYGRPS
                     ATPDEVVFAARLARIHETVERLPQGYETIVGEQGATLSEGERQRITIARAILRNAPIL
                     ILDEPTSSVDAETEALIMDGLNELTAGRTTFIIAHRLSTVRQADLILVLRSGEIVEQG
                     TFEELIARDGPFSVLYHTQFGLGGEKENGLFG"
     gene            7218..8180
                     /locus_tag="PLM79_11375"
     CDS             7218..8180
                     /locus_tag="PLM79_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOV86957.1"
                     /translation="MHLRKRCAVTGGAGFLGSHLCERLLRDGGDVLCLDNFYTGSKRN
                     ILHLLSDPYFELYRHDITYPLYVEVDEIYNLACPASPIHYQNDPVQTTKVNVHGSINM
                     LGLAKRLKAKILQASTSEVYGDPKVHPQNESYWGHVNPIGRRSCYDEGKRCAETLFFD
                     YHRQHGLKIKVARIFNTYGPRMHPNDGRVVSNFIVQALKGEPITIYGDGSQTRSFCYV
                     DDLVDGLVRLMNSPPDFTGPVNLGNPGEFTILELAEKVKEMTGSSSQFIFKELPSDDP
                     VMRRPDIGLANKVLGWEPKVPLHEGLRKTVDYFDDLLRTGNMAD"
     gene            8481..8969
                     /locus_tag="PLM79_11380"
     CDS             8481..8969
                     /locus_tag="PLM79_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86958.1"
                     /translation="MRKRLLGLTLLFLWMSADPVFSGALSEIELTDGSLLRAEIVSMD
                     FGKITLRSPTLGTIQADKSRVRNIRILADSLGATQRRTAADTSVGEQVKILQEQIASD
                     QELMTRILDLQNDPDFQSILADPEVMQAVNSGDVQKLVSNPKFMRLLDKPSVRGITQG
                     MK"
     gene            complement(9080..9859)
                     /gene="scpB"
                     /locus_tag="PLM79_11385"
     CDS             complement(9080..9859)
                     /gene="scpB"
                     /locus_tag="PLM79_11385"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF008506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HOV86959.1"
                     /translation="MDLISKHLEDKIGTITFRNERKRNCLSRVLLEQLMEALEDLKAQ
                     KTQVVVLRAPAGSKVWSAGLDIGEFPKSGRDPLAYDDPLEQVLRAIQRFPAPVIAMVE
                     GSVWGGACDLAFTCDIVVGTGSASFAITPAKVGIPYNASGILHFVNIVGLHIAKEMFF
                     TAAPITAERAEKYGILNRLVPPEELESVTYGMARQIVRNSPLAISVIKEQLRILGNSH
                     PLSPETFERIQGLRRRVYDSEDFIEGKRAFLEKRAPVFRGE"
     gene            10159..11055
                     /locus_tag="PLM79_11390"
     CDS             10159..11055
                     /locus_tag="PLM79_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86960.1"
                     /translation="MSKHPIRLCFILLWFWIAGLPAGGHGTDLPPSANQEEMETTVLH
                     VDKYAVYGPNLIFYWDPGMSKQKIGALTSAAERLRNKKARITYRVREDAPKRSRFILM
                     DIAAAPKESKPSTEAPQAKAPSEERSRMGPRGEPDSDLDREEGPASLEPEPDLPLFDD
                     EETPRTTFLPVTISRKEILRFIEKCMDANTRKNLDGALACYDHEVDYYGKGKVDKEFI
                     RKDKGYYFRQWDTISSSIEGDVVIIVTDEPGVQIAKFVSLFEVTKGSKTVRGRAENLW
                     KILKVDGQLKIMDEKQRILPPR"
     gene            complement(11327..11842)
                     /locus_tag="PLM79_11395"
     CDS             complement(11327..11842)
                     /locus_tag="PLM79_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86961.1"
                     /translation="MSKKMWLLGLALIFAVASSAAAQTTPTAVPVDVKPNVCPNPLNL
                     RSSGYLMIGILGTADLDVNNVDPVSVRVEGVAPKASIINVDKATPYEPFLGKTGPTQC
                     NEDGADGYPDLILKFRSQQILKALRDQGVELQKGTPVVLHVTGALRDGTPIEGEDVVV
                     IKGKKPKKSRD"
     regulatory      complement(12042..12126)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            12974..13204
                     /locus_tag="PLM79_11400"
     CDS             12974..13204
                     /locus_tag="PLM79_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86962.1"
                     /translation="MVRLFKCKAVGVNVLELTVEERFGRADARCRLAYENIDLPDEPL
                     AACSTGREVPIPGDRPRLIRFKDTDQGGWRVY"
     gene            13500..14738
                     /locus_tag="PLM79_11405"
     CDS             13500..14738
                     /locus_tag="PLM79_11405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOV86963.1"
                     /translation="MKETFQKFADLFHRVDPSATFAFEFWDGSSSGFGSHPAVVLRLK
                     TPKAAEQILKKGFYGLGEAYVSGRAEIDGDLRELLRLGFASRIEESGPHWLQLLQNFR
                     HQCMTRISPHRPDKDVPRHYDLGDELFALFLDPTMTYSCAYFKSGDVSLEEAQLSKYD
                     HIARKLLLQPGERLLDVGCGWGGMLIFAARNYQTDGLGNTLSRNQYEYARRKILELGL
                     NGRVEVVLQDYRQCTGKFDKFVSIGMLEHVGKDFLGDYMKKVAGLLKKGGLGLLHTIG
                     KETESITDPWITRYIFPGGYLPSLAEIVGNMGRAGLSILDIENLRLHYSRTLDHWAAN
                     FDRNLDRARRLFGEKFVRMWRFYLLSCSAGFRYGATRVYQVLFSNGLNNDLPTTRDHV
                     YLGESPGWAAFEEPLASCAS"
     gene            complement(14839..14915)
                     /locus_tag="PLM79_11410"
     tRNA            complement(14839..14915)
                     /locus_tag="PLM79_11410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14879..14881),aa:Arg,seq:cct)
     gene            complement(15092..15745)
                     /locus_tag="PLM79_11415"
     CDS             complement(15092..15745)
                     /locus_tag="PLM79_11415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HOV86964.1"
                     /translation="MRRGTPVFGTVMVLIMVGLFQASHAAKTAYVTETHEIALRTGPS
                     TQNRVLGMVPPGTGVEVIKTRNEWSQVRFTGPSGESKEGWVQNRFLSDSPTKELETEI
                     LALKEKIAGLEQEKELLSQKEREGKDKLARLVTDYEVLKSGSANFLKLKSEYDSLKTA
                     LNSAQDNIQKLIQENKNLKLSERIKWFVAGALVLFVGFVFGMAAGRYQRKRRATQYR"
     gene            16065..>16307
                     /locus_tag="PLM79_11420"
     CDS             16065..>16307
                     /locus_tag="PLM79_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOV86965.1"
                     /translation="MLVDTHAHLDFPEFVHDIPGVLHRAHEMGVTTILTIGIDLESSL
                     EAARLAQQYDSIFASAGIHPHGARSLSNEDCDTLKAL"
BASE COUNT         3313 a         4893 c         4720 g         3381 t
ORIGIN      
        1 cacggcctcg gacaaggtgg gatgcccatg gagcgttccg gccggatcct gggggctcac
       61 atcctgggcc cccgggcctc ggacctgatt gcggaatgcg tgctggccat gcaggtggga
      121 gggagtgcgg aagatctcgc cggaacgatc catgggcatc ccaccttgtc cgaggccgtg
      181 catgaggccg ccgccgccgt gagaaggtgt tccatctacg ctccgcggcc gtagtcctct
      241 tctccacgac tctatttccc acaaattcgc tttcccagat ccgcgggggt atgttatctt
      301 cccgctggtc tgtgaatggg ctccaagccc gcggcaagcc atgcacgaag tctgccgggg
      361 gtggggttgc acccgggggg accccggaga gggagggctc cacgggccgg ggaaacacga
      421 gccccttgcg gatggcctgc ccaggaatcg aggatgaata acaaactccg aatcgttgtc
      481 accggacttg cggcaaccta tcctttcggc ggggtctttt gggactatct gcaatatgtg
      541 ttgggctttc gccgactcgg tcacgaggtt ctgtacctgg aagacaccgg gaaatggtgt
      601 tacgaccctt cggccgggac ctttgtggaa gacggaagga acaatgcggc ctttctttcc
      661 gccgaactgc accggctgga cccttccctt gaagggcatt ggttctaccg ggactcgacg
      721 gggaaaacct atgggcggca atggcgggat gtggtctcgt tttgccgtag cgccgatctt
      781 ttcctgcatg tttccgcatc ctgttggatg cgcgaagagt atttcgccgc atcccgggta
      841 gccttcatcg acagcgatcc catgtacacc caggcatcca tccccggcta cctggacgga
      901 agcctcgatg aggaagcccg atcccgggtc gataccctgc ttcaacacga cgtgttcttt
      961 accttcggcg agaatgtggg agcttccgac tgcaggattc ccacggagct cttccgctgg
     1021 attcccactc ggcagcccgt ggtgctggac tgcttcaggg accaccggat tccggtgtcc
     1081 gcccgcaggc gggtcctcac caccgtggcg tcctgggaac cgtccgagaa gggccccgtg
     1141 gtgaggggag ttgcttacgg gggcaagagc atcgaattca accggttcat ggatcttccc
     1201 tcccgcagtg tgttgcctct ggaactggcc atgagcggtc gcgctccccg ggacaggctg
     1261 acggctgcgg gctggggggt gcgggacggg tacgaagtct ctgcaaatcc ttggatttac
     1321 cgggactatc tggccgactc cctgggggaa tggagtgtgg ccaagaacgc ttacgttctc
     1381 agccgaagcg ggtggttctc ctgcagaact gcctgttacc tcgccctttc ggtgcccgcc
     1441 gtggtccagg acacgggatt tgaccggttc attcccacgg gagagggcct tttcgccttc
     1501 aatacgccgg aggaagccgt cgaagccatt tcccggattg agagcgagcc cgaaagacat
     1561 gctgcggcgg ctgcagccgt cgccgccgag tacttcgatt cggacatggt gctccgacaa
     1621 ctgatcgaag atgccttttc gtcctcgtcc aattgaatcc aagggcatcc aacaagaggg
     1681 aggccaagaa acgatcccgt tcgggatgag gttggatggg gaccggcggc aaattcatga
     1741 ccccagttcc ctggagagaa ctcgacatgt acagtcccat cgtcttccgc tgcctgctcg
     1801 ccgtcctcgg ggtggtcctt tgggccgccg caacgtctcc ctgccgcgcc ggaatggtca
     1861 gcctcacctt cgatgacggc ctcagtagtg tttacagcga ggcgtatccg gtactcaagc
     1921 ggcacaatct caaagccacc gtggggatgg tggtgaaccg catcacctcg ggcaacgacg
     1981 actacatcag cctccaagag gcccacgaac tccaagacgc cggatgggag atcgcgtctc
     2041 acggcttgac ccaccagagg cccatcgata tcccgcaata cttcgaagac gaggtggtgg
     2101 agggttggga accggacggc accaatgccg aggtctatga agccaagtac gattaccaca
     2161 gcgtggcggg gttgctggaa aacggaaagc ccctcaagga attcacccga ctggaggacg
     2221 tccggcgagc caagggcagt ttctatttcg accgactcat cggggccctc cacgtccacc
     2281 ctttcaaccc gatgaacgcc gaaagggaag acattcgatc ttattcttac cagcgggaac
     2341 tgtgcgaatc caagatcgcc ctggaaaaac tgggcttcaa gatcagcacc tacatcactc
     2401 cgttcaacta ttggaccgag gagatggctt ccctcagcaa gctctattat tcccaggtgg
     2461 ccaacgggaa aaacatggcc aaccgcaagg aaacctgcga cctcaactgg ttgtatcgtt
     2521 tcaatgtcct cagcatggga aaaactcagg acgccgtccg attgatccaa aagcacgccg
     2581 tcgagcagga cgagtgggtg attttttgtc tgcacggaat cgaagatgag atggggtggg
     2641 agccatggtc cgcggataac ctggaagaac tggcagcctg gctgcagaag aaccagatcc
     2701 gagtggtgac cgtttccgag ggagccgccc tctattcatc cgtgccacag gcacgaagga
     2761 ggtagggatc ggttgaaggg acgaccgctc aagatcgtgg ttgccggatt cgccattgga
     2821 ttccccatgg gcggacaact ctggatgatg atgcattatg tgctgggttt gaaacaactg
     2881 gggcacgaag tgcttttcct cgaggatacc tccgactggt cctacgccta cgatcccgtg
     2941 cgccggtcct atgatgtgga ttccagctac ggacggcgag tgctggagga tttcttccgc
     3001 cgcaacgggc tcgagggcca atgggtctac aacagcgccc tggaaaaccg gatttacggg
     3061 aggactcggc aggaactgga tcggttctgc tccgaggcgg acctgtggtt gagtctttcg
     3121 ggaaccaatc ccctgcgcga aaattacatg cggtgcaggg tcaaggccgt catcgatacg
     3181 gaccccgttt ttacccagtt gaagattcgg gacgatgaaa tcactcggga ttacttcaag
     3241 gcccacgacg tttgcttcac ttggggctac aatctaccat ccctgaaaac ccgagtcccc
     3301 ctttccggca tcgactggaa accgactctg ccccccattc tgctggaaaa gtgggctccg
     3361 gtatctcccc ccggaaggtc gtacaacacc atcgggttct gggacacgaa ggggcgggac
     3421 atcgtcttgg acggagaagt cttttcctgg cgcaagcgac cgaaatatga acgactcatc
     3481 gatcttcccg gccaactccc cggcctgccc ctggagattg ctttcatggg aatcgaggac
     3541 gacggacctc gttatgccga ccatgggtgg atccttcgag accccgtggc cgtatctgcg
     3601 gatccgtaca attacatgga ctacatcaag aattcccggg ccgagttcac ggtggccaag
     3661 gaccagaacg tcaagctcaa gagcgggtgg ttttccgacc ggagcgcatc ctatcttgcg
     3721 gcagggcgcc ccgttgtgac cgaagacacg gggttttccg cctatcttcc cgtgggagag
     3781 gggatttttc cctacgaaac gagggaggag gccatcgaga gcatacgaac catcgaacgg
     3841 gatcccgttc accattcccg ggcggccctg caccttgccg aggaacactt cgacgctcga
     3901 aaggtgctca cggaaatcct tcgtgaggtg gggctcgcct gatcctgcaa cccgggaacg
     3961 gcttcgggga gccaaatcct gcggggagaa ccatgagagg ggcggcgctg gaccccggcc
     4021 cgacgcaacg gctggcctgg gtggatgtga ccaaggggat ttccatcctc tggatcgtct
     4081 ttttccatta tttcaagacc tatgccaagg gccgttatcc ctggccgttg gacccggggt
     4141 tcctgtccga atccctggcc cggtgcgaat tcgcctccct tctttccaac ctggtttgcc
     4201 tggccgacag cctctttgcc gccgtctccc acctgggctt ccacgcggtg ggggtcttcc
     4261 tactcatgag cgggttcggg ctcactttct ccctggccaa gaccggaaac cccgccgaag
     4321 gctgggcggg atggtaccgg agccgcctgc tccgactctt ccccatgtac tggatcgctc
     4381 acttcatcta cgtggtttcc cccctcgtgg cgcgaccgga acccatcgat tcccgcttct
     4441 ttctgagctt tctgggaaac cgcgtgtatc ccatcgagac cctgttctac tacatgaacc
     4501 cggcatggtg gttcgtggga ctcctcctgc agctctacct ggtgtttccc ctcctctttc
     4561 gcatgctcca aaaactgggc attgggcgct tcatcctggt gtccaccctg atcaccctcc
     4621 tgagccggac attgctgctc ttcgtcctcc aggccaatgg agactacctt caagggggct
     4681 ttttcggcag tcggcttatc gagtttgcgg cgggaatggc cctgggttac gcctaccgta
     4741 agcaacacga ccttgcggat tcttcctctt caaccggaaa accctggcgg caggggcctg
     4801 cctctatgcc ctgggaatcg agagcaacgt ccgattatgg tcttacgtgt ttgcggaccc
     4861 tctcatcggc gtgggtcttt ccatcctgtt gattcacttg agcgaagctg tgggcaatcg
     4921 attggatcaa ggggggcggg tattggcccg cgtcggggtg tactcctacg ggctctacct
     4981 ccttcaccag ccttacctca taaccctagg ggagaacacg cggcaactgg cgatgatccc
     5041 ctcgaccctc ctggccttgg cggtcatcac agccatcgcc ctgccctgca tggtcctgga
     5101 aaaatccgtg aaccgactca tgcggcactg gtaacgccct cggcctccgt catccgaaca
     5161 gcccgttctc cttttcccct cccaggccga actgggtgtg atagagcacg gaaaagggac
     5221 cgtcccgggc aatcaactcc tcgaaggtcc cctgctccac aatctctccg gaacgaagga
     5281 ccaggatgag gtccgcctgg cgcacggtgg agaggcggtg ggcgatgatg aaggtggtgc
     5341 gcccggccgt gagttcgttg aggccgtcca tgatgagggc ttccgtctcc gcgtccacgg
     5401 aagaagtcgg ttcgtccaga atgaggatgg gggcgtttct caaaatagca cgggcaatgg
     5461 tgattctctg tcgttctccc tcggataagg tggctccctg ctccccgacg atggtttcat
     5521 atccctgggg aagacgctcg acggtctcgt gaatccttgc cagccgcgcc gcgaagacaa
     5581 cctcgtccgg ggtggccgaa ggtctcccgt atgcgatgtt ttcccggatg gtcatgggaa
     5641 aaaccagggg aggctgcagc accatggaga tctgactcct tagggacttc aattggaatt
     5701 ctttgacgtc cgtcccatca atgaggatcc gccccgattg cgggtcgtag aaccggggaa
     5761 tgaggctcac cagggtggac tttcccacac ccgtggggcc tacgatggcc accttctgcc
     5821 ccttgcgaac cttcaaatcg atcttccgaa gcaccggctg ctcgggaacg taatggaaag
     5881 agaccccttg gacctcgatt tctccccggg ccccgtcctc ggaaaatgtc ctggttcctt
     5941 ccggcaaatc cctttccacc tccaggattt cgaaaaccct ctggaccccg actttggaac
     6001 cttgaagcat tccccacgtc tggctgatgg tgttgatggg accgtaaagg gaagcaagat
     6061 aagaggtgaa cacaacgatt tcgccgacgc tcagggtccc ggacatgacg tgccgggccc
     6121 ccacccacac caccacggcg gtcccgacgg ccatgatcac gttgacgagc ccggaataga
     6181 aggtctggag cacgtagagg cgcaaactgg atcccaggct ctgttcgctg gccctcatga
     6241 accgtcggtg ctcttcttcc tccttggtaa acgcctggat cacccgcatg gccgacatgg
     6301 cccgttggac cagggagtag acctcgcttt cctgctgccg agcggtggtc gccgccgatg
     6361 tcagggtctt gccgaacacg gagatggaca agaggagcaa agggcacact cccagggcaa
     6421 gaagggtcag cacccaatcc attcgaatca tgatcacgaa catgcccacc aggagaacca
     6481 ctgcggaaac aatggggaaa accccgttca tggtcagact ctggatggag tacgtgtctg
     6541 cggccactcg gtacagcagg tcccccacct ggcggcgact gtgaaaggac agggacaacc
     6601 gttggagatg gttgtacagg tcccctctca ggtcgttgac catcctttgc cctatcttga
     6661 tggtggtgta gttgttcaac aacaggatgc ttcccaggag gacgtagatg accaccatcc
     6721 cgatgcaggc aacgaccagg agggtatcct gctgcaggcc gcccagccaa atccagtcca
     6781 ggggttgatt gctcaggaca ttgtcgatga tgatcttcaa aggccagggt ttcagaagct
     6841 cacaggcact gatgagaaac acctggccca gagccgcaag aaaagggacc cggtaaggcc
     6901 aaaggtaggg aaagactttc cggatggtgg ggaaactgct ttgcataaga ctcgatcact
     6961 gcctcaaaac gttggagttg aacccaaagg acgcgccctc tcacggtttc ttgccgggag
     7021 ccgccgcgaa acggttggac ggacactaga ccaataggcc cccgtaatca acagaaattc
     7081 atgaaccagt tctcaattgt caaggaaggg aacgtgaatt agactggagc cttacagagg
     7141 tggtcgaacg gacggcccga ggggcctgtg aaagagcacc taacagccgg gtttgtttcg
     7201 aaaagaaagg agctttgatg catttgcgta aacggtgtgc tgtgaccggc ggcgccggtt
     7261 ttttgggttc acacttgtgc gaacggctgc tcagggacgg aggggatgtg ctttgcctgg
     7321 acaattttta caccggaagc aagagaaaca tcctgcacct gctttccgat ccctacttcg
     7381 aattgtatcg tcacgacatc acgtatccct tgtacgtgga agtggacgag atctacaacc
     7441 tggcttgccc ggcctccccc atccattacc agaacgatcc cgttcagacg acgaaagtga
     7501 acgtccacgg ctccatcaac atgctgggac tggccaagcg actcaaggca aagattcttc
     7561 aggcatccac ctccgaggtg tacggagatc ccaaagtcca tccccaaaac gaatcctatt
     7621 ggggtcacgt gaatcccatc ggccgcaggt cctgctacga tgagggaaag cgttgcgcgg
     7681 agactctctt cttcgactac catcgtcagc acggcctcaa gatcaaggtg gcccgcattt
     7741 tcaacaccta cggcccccgc atgcatccca acgacgggcg cgtcgtttcc aacttcatcg
     7801 tccaggccct caaaggggaa cccatcacca tctacgggga cggatcccaa acgcgatcct
     7861 tctgttacgt ggacgacctg gtggatgggc ttgtacgcct catgaattcc cccccggatt
     7921 ttaccggccc ggtgaacctg ggcaacccgg gagagttcac cattctcgaa ctggccgaaa
     7981 aggtgaagga gatgacgggg tcctcttccc aattcatttt caaggaactg cccagcgatg
     8041 atcccgtcat gcggcgtccg gacattggcc tggccaataa ggtcctgggc tgggaaccca
     8101 aggtgccgtt gcacgaagga ttgcgaaaga ccgtcgatta ctttgacgac cttctacgca
     8161 cggggaacat ggccgattag gcgcaactgc ccggaaaagg gctccccggg acgcgtcccc
     8221 aacgatggtc gtgccggggc aggccgcacc ccatgaagac cttccggatg ccctgccgtc
     8281 cccaaaggac ggcagcactc cttcagggtc ggttgcgagc ctcgagcccg gggcggctcg
     8341 cctctcgaac ggcgtttgtc ggcaacggtt tgtggggagc ttcactcgag atggatccgc
     8401 agctcgaccc caaccttcct gccgcgggag agggtcctct ttcttgcccc tgacgggaac
     8461 gatcggagga gacggccctc ttgagaaaac ggcttttagg actgacgctc ttgttcctgt
     8521 ggatgtcggc ggacccggtc ttttccggtg cgctctccga gatcgaattg accgacggga
     8581 gcctccttcg ggccgaaatc gtatccatgg atttcgggaa gatcaccctc cggtccccga
     8641 ccctgggaac cattcaggcg gacaagtccc gggttcgaaa cattcggatt cttgccgatt
     8701 ccctgggtgc gacccaaagg cggacggcag cggacacgtc cgttggagag caggtgaaga
     8761 tcctgcagga gcaaattgcc tcggaccagg aactcatgac caggatcctg gatttgcaaa
     8821 acgacccgga cttccaatcc atcctggcgg atccggaagt gatgcaggcc gtgaactccg
     8881 gggacgtcca aaaactggtc tccaatccaa agttcatgag gttgctggac aagccctccg
     8941 ttcgcgggat cacccagggc atgaaatgac cccgggccgg tttccatgga aatcagggac
     9001 ccccgtttgc cccacgatgg ctgcggcgac aaaagtcccg gggcaaggcg cattcttgcg
     9061 cagcctttgc gcctcccctt cattcccccc ggaagaccgg ggcgcgtttt tccaggaagg
     9121 cccgtttgcc ctcgatgaag tcttcgctgt cgtacacccg gcggcgcaac ccttgaatcc
     9181 gctcgaacgt ctccgggctc aggggatggg aatttcccag gatgcgcaac tgttccttga
     9241 ttaccgagat ggcgaggggg gagttccgaa cgatctgtcg cgccatgccg taagtgaccg
     9301 actccagttc ttcgggggga accagccggt tgaggatgcc gtatttttcc gcccgttcgg
     9361 cggtgattgg agccgcagtg aagaacattt ccttggcgat gtgaagaccg acgatgttca
     9421 cgaaatgaag aatgccggag gcgttatagg ggatccccac cttggcgggg gtgatggcga
     9481 agctagccga gccggttccc accacgatgt cgcacgtgaa ggccaggtcg caggctcccc
     9541 cccacacact gccttccacc atggcaatga ccggagccgg aaaccgctgg atggcccgca
     9601 acacctgctc cagagggtcg tcgtaagcaa gagggtccct tccggacttg ggaaactcgc
     9661 cgatgtccag gcccgccgac cataccttgg agcctgcagg ggctcgaagc accacgactt
     9721 gggtcttttg ggccttcaag tcttccagtg cctccatcaa ttgctcgagg agaactctgc
     9781 tcaggcaatt gcgcttccgt tcgtttcgaa aggtgatggt tccgatcttg tcttccaggt
     9841 gtttcgaaat gaggtccatg cttccactgc ctccgttagg tccgacaccc tcccgaaaaa
     9901 tgagggcgtt gcctctttcc atggcgcgat atcgggggcg gggacaaccc ccctcccaaa
     9961 aatttctttt gaaagaaatc gacatgcaca ttctgcacac catttatttg acaattctca
    10021 aaacttgcaa taaaaattgc acggtttttc cgcacgactc acaaaatgag tagggcgagg
    10081 ggcttggatt tacaaaatcc aggcctttcc actttctggg acatgctttt tttcaggagt
    10141 cgtcaacaga ggcacaggat gtcgaagcac ccaatcaggc tttgtttcat actactctgg
    10201 ttctggatcg ccgggttgcc cgccggagga catggaaccg acctccctcc ttccgccaac
    10261 caggaggaaa tggaaaccac ggtccttcac gtggacaaat acgccgtgta cggccccaat
    10321 ctcatcttct actgggaccc cgggatgagc aagcagaaga tcggggccct gacttctgcg
    10381 gcggagcggc ttcgcaataa gaaagcccgc atcacgtatc gagtacgcga ggatgcaccc
    10441 aaacgcagcc gattcattct catggatatc gcggcggctc ccaaggaatc caagccctcg
    10501 accgaagccc ctcaggccaa ggctccctcg gaggaaagga gccggatggg accccggggc
    10561 gagcccgact cggacttgga ccgggaggaa ggccccgcct ccctggaacc cgaaccggac
    10621 ctgcctctgt tcgacgacga agagaccccc cggaccacgt tcctgcccgt caccatttcc
    10681 aggaaggaaa tcctgcggtt catcgagaaa tgcatggatg ccaacacccg caagaacctc
    10741 gacggggccc ttgcatgcta cgaccacgag gtagactatt acggcaaggg gaaggtggac
    10801 aaggagttca tccgcaagga caaaggctac tactttcgac aatgggatac catatcttcc
    10861 tccatcgagg gggacgtggt gatcattgtg accgacgagc cgggggtaca gatcgccaag
    10921 ttcgtgtccc tgttcgaagt cacgaagggg tccaagaccg ttcgcggccg agcggaaaac
    10981 ctctggaaga tcctcaaggt cgacgggcaa ctcaagatca tggacgaaaa gcaacggatc
    11041 ctccctcccc gatgacgagc ctcaagggta ggacgaagag acgggaatcc gggtgtgggg
    11101 aaggcaagga cagacacggg tcttcgaagt tgggctcggt tctccccccc ctcctccgcc
    11161 aaggaagccc cggcttcgcc gatgcttgca gtgcgggaag agaagaaggc cggactgatc
    11221 gacggcctcc tgtgcgtgaa ggcatgcatc ttccgcaacg gaagtatagg aagagaagaa
    11281 ggccggatcg agctccggcc ttctggggcg gcgcgggtgg ccgagactaa tctctggact
    11341 tcttgggttt cttgcccttg atcaccacca catcttcgcc ttcgatggga gttccgtccc
    11401 tcaaagcacc ggtcacatga agaacgaccg gagttccttt ttgcagttcc actccctggt
    11461 ctctcaaggc cttcagaatc tgctgggagc gaaacttcag gatgaggtcc gggtatccgt
    11521 cggcaccgtc ctcattgcac tgggtgggtc ccgttttgcc gaggaacggt tcgtacggag
    11581 tggccttgtc tacgttgatg atggaggctt tcggtgccac tccctccacc cggacggata
    11641 ccgggtccac attgttcaca tccagatccg ccgttcccag gattccgatc atcaggtagc
    11701 cgctgcttcg cagattcaat ggattgggac aaacattggg cttcacgtct acgggcacgg
    11761 cggtgggagt ggtttgagcc gccgccgagc ttgctacggc aaagataaga gccaaaccca
    11821 aaagccacat ctttttcgac atcctacccc tcattgggat cctcctcggt tgggcgagtt
    11881 tgttccctcg gcccggacga tcgtaaaacc gggcctctcc ttcttggtgg tttcgccggg
    11941 agatcctaag ggaaactgct gcgagacaaa cctgccatgg cgctcctccc tggttgcaaa
    12001 aggttgacga tgagcggcac agcttgcgga tgctgtgttc gcctggcagc cctgctgccg
    12061 tatcccggcg gacttaggcc agcggctttg cgccccgccc tttcgggcgg tttgccctgt
    12121 acaaacgaaa cagcggcttt atgaactgtc ggtcgggatt ccaatcccct tgagcggatt
    12181 tttcacaacc atgccctgag gtccttcgct tccccgcttc gccgccgcct tgactttcag
    12241 caagaacggt gccggttgca tcccgcaacc cggctgcccg tgaattcccg tccttttcgg
    12301 gacggcgtta tgggggttgg tcgggaatgg aggcacccct tccctaaacg caatccttgg
    12361 acgaaggtga ggaccctact tcccgatgaa gcgaccttct ccaagtttcg tcgtttcggc
    12421 cgtatttcgt caaggcaacc gttgtttgga ggcaatcgcg gccggcttgc ccccttcgag
    12481 tttccgggcc tctgcaaccc gcgcaagcgg tctgttccgg attttgggcg tttacgtttc
    12541 ctaatcatga aaaaactttt gacttccccc cagggctttc aaaatgcgga agcggcaatc
    12601 cggggcacag gggcggaacc ggtttgaaag caagacacag gaaaactccg tgaagcaggt
    12661 cggttggcgg atgttgttgg cagggatcct cgttgcagga ctcccttggg tggaggctgc
    12721 ggaagagtag gacaaggaac gggcgtccca gctcatcgcg acccacctga aattgcccca
    12781 attcggccgt tcttccggca ttcaggcatt ttccggcgca aatgcccggt acgtcccgca
    12841 tgtaagaaac ccggaatttg gtcaagtgcg ccctgccggc gcccttcgac caaaaccgtt
    12901 atgccgcccc acggacaggg gatatccctg gacctggagg ggacctggta ggcggctccc
    12961 cagggtcaaa catttggtac ggcttttcaa gtgcaaggcc gttggcgtca acgtgctgga
    13021 gttgacggtc gaggaaagat tcggcagagc ggacgcccgg tgccggcttg cctacgaaaa
    13081 tatcgatctt cccgacgaac ctctggccgc ctgttcaacc ggcagggaag tcccaatccc
    13141 gggcgaccgt ccccggctga ttcgcttcaa ggataccgat caaggcggat ggcgggtcta
    13201 ctagggaacc cccttccgat tcctcccggc agccccgggg cccaggcccg aggtcgaccg
    13261 gccgggtcct actacggccg aaatgtcccg cccccggaga ttttcggaga attttacacc
    13321 cggcgcctca aggcttgact caaaaacctc ccgcgtttta tcattgaaac aagcggaagc
    13381 tcccgttcct cctacaattc ctccgacgac tacccggaaa ttgctccgtc cttgaagatg
    13441 gaggggtcta aaggtccgtt cttggcttca tgggaatcag gaatgagggg ggcaccttta
    13501 tgaaagaaac cttccaaaaa ttcgccgatc tctttcatcg tgtggatccc tcggcaacct
    13561 ttgcttttga attttgggac ggaagcagct ccgggtttgg gagtcatccg gccgtcgtgc
    13621 ttcgtctcaa aactccgaag gcggccgagc agatcctcaa gaagggcttt tatggcctgg
    13681 gagaagcgta cgtttcgggg agagcggaga tagacggcga ccttcgcgaa ctgctgcgcc
    13741 tggggtttgc ctcccgaatc gaggaatccg gaccccattg gttgcagctg cttcagaact
    13801 ttcgccacca gtgcatgacc aggatttctc cgcaccgccc cgacaaagac gtccctcggc
    13861 actacgacct gggagatgag ctcttcgctc ttttcctgga cccgaccatg acgtactcct
    13921 gcgcctattt caaatccggg gacgtttccc ttgaagaggc ccaactgagc aagtacgatc
    13981 acatcgcccg aaagctcctt cttcaacccg gcgaaaggct gctcgatgtg ggatgcgggt
    14041 ggggaggcat gctcattttc gccgcccgga actaccagac cgacggcctg ggcaacaccc
    14101 tttcacgcaa ccaatacgag tatgcccgtc gcaaaatcct cgaattgggc ctcaacgggc
    14161 gtgtggaagt ggtgctccag gattatcgtc aatgcacggg aaaattcgac aagtttgttt
    14221 ccatcgggat gctcgagcac gtgggcaagg acttcctggg agattacatg aaaaaggtcg
    14281 ccgggcttct caagaaaggc ggactgggcc tgttgcacac aatcgggaag gagaccgagt
    14341 ccatcacgga cccgtggatc acccggtaca tattccccgg cggctatctc cccagcctcg
    14401 ccgagatcgt cgggaacatg gggcgtgcag ggctaagcat cttggacata gagaatctcc
    14461 gcctgcacta cagccgcacc ttggatcact gggcagccaa cttcgacagg aatctggacc
    14521 gggcgagaag gttgtttggg gaaaagttcg ttcgaatgtg gaggttctac ctgttgtcct
    14581 gctcggcagg cttccgttac ggggccaccc gcgtctacca ggtcctgttt tccaacgggc
    14641 tgaacaacga cctgccgacg actcgagatc acgtctacct gggggaaagt ccggggtggg
    14701 cggctttcga ggaacccctg gcgagctgtg cgtcctgagc ggggtcgagc aagggtcgtc
    14761 cgggccccct cttccataaa cccgcatgca tagaacacga ggggggctgc ggccgtcccc
    14821 gcagcccccc ggctgatctg gtgcccccga caggactcga acctgtggca ccgagattag
    14881 gaatctcgtg ctctatccgc ctgagctacg ggggcattct gagtggctct ctccttagca
    14941 tacgggtctt tttccgtcaa ccggttcatc cccgatgcaa tcaagtcctt ggggaacgcg
    15001 gatccccgga acctctgccc gcaaccgcca tgagaaaccg ccccccagcc ggacccgccc
    15061 ctcgtcgggt gcaccgggac ccaacctttt ttcagcggta ctgggtggcg cggcgcttcc
    15121 tctggtatcg cccggcagcc atcccgaaca caaacccgac gaaaagaacc aaggctcccg
    15181 ctacgaacca cttgatccgc tcggagagct tcaaattctt gttctcctgg atcaatttct
    15241 gaatgttgtc ctgggccgaa ttcaacgcgg ttttcaagga atcgtattcg gacttcaatt
    15301 tcaggaaatt ggcggaaccg ctcttgagca cctcgtaatc cgtcaccagc cgggcaagct
    15361 tgtctttgcc ctcccgctct ttttggctca gaagctcctt ttcttgttcc agcccggcga
    15421 tcttttcctt gagggccagg atttccgttt ccagttcctt ggtggggctg tcgctcagga
    15481 agcggttctg tacccagcct tccttggatt ctcccgaggg cccggtgaat cgcacctggg
    15541 accattcgtt tcgtgtcttg atcacctcca ctcccgtgcc gggaggcacc atgcccagga
    15601 ccctgttctg ggtgctgggc cccgtgcgaa gggcgatctc gtgcgtttcc gtcacgtacg
    15661 cggtctttgc agcgtgactt gcctgaaaca ggccaaccat gataagtacc atcaccgtgc
    15721 cgaaaaccgg ggtccctcgt cgcattggcc catcctgtcc tttcgtggtt gggttgatcc
    15781 ggcttgggtg cgccggagcg gagttttgga ttatagtgag acccggcgcc cgtagcaagg
    15841 caaaacggcc gcgcgcgccg gaccgggcct ctcgagggcg ggcaatgacg cggaggtcct
    15901 gcgccctccg gcgaactgcg gagcaggctc ccggccggga aaacagtatg aaattccggt
    15961 attggcatag gccggaaatg acgccggggc gtggatcgac ggcatcgagg acgggccctt
    16021 ttcggcccgg gagcgtttgt gtttacttgt gttcggaggg ctttgtgctg gttgataccc
    16081 acgctcacct ggatttcccc gagtttgtcc acgacatccc cggtgtgctg caccgtgccc
    16141 acgagatggg cgtcaccacg atcctcacca tcgggatcga cttggaaagc agcctcgagg
    16201 ccgcccggtt ggctcaacaa tacgactcga tctttgcctc ggcgggaatc catcctcatg
    16261 gggcccgaag cctcagcaac gaagattgcg acaccctcaa ggccctg
//
LOCUS       DAOOVC010000086        16160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2428_length_16160_cov_5.756473, whole genome shotgun sequence.
ACCESSION   DAOOVC010000086 DAOOVC010000000
VERSION     DAOOVC010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16160
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2428_length_16160_cov_5.756473"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="PLM79_11425"
     CDS             complement(<1..1569)
                     /locus_tag="PLM79_11425"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV86966.1"
                     /translation="MATVKLTINGREVEIEQGSTILQAAKKAGIYVPTLCYHPFLPLE
                     EACRICVVEEIRGGWSSQVAACVYPVRNGMVIETDSEKVLDARRTIMELLLSNHPNDC
                     MTCYATGECELQDMAHRYRVKDSVFRGARQSHPVDPDPNPVLHLDMNKCILCRRCIRA
                     CHDVEGADIWTKVGRGFEQRISTAFDLPLDEAGCESCGHCADFCPVDAIGFRAGRGTV
                     RAWQYRRSSTVCLQCASGCRAEYDTFHGKVVRVLGDFNSPANSGALCKRGRFNLDFMN
                     SPERLTTAFVRNESGTPEAVSVEEGLRAAAQGLKRVRDQRGENSLAVLCGGSLTNEEY
                     YLAQKLARGALATNHVDSVGGPWQQAMADGLSGVLGFGASTNPLSDIARAGAVLVLGS
                     DTLEKHAIAALRARKAAREGAVLLVAHPEKVLLTKTATLHLPMKNGSEGALLAGILKT
                     VVAEGLHDRNYVAAHTQDFQKLEKSLEKINLSSVAAQTGIPEERILKAARLFAGRKPA
                     CLVYGIDPGTSPDNE"
     gene            complement(1641..2510)
                     /locus_tag="PLM79_11430"
     CDS             complement(1641..2510)
                     /locus_tag="PLM79_11430"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV86967.1"
                     /translation="MRASLDSLKDLGRVIRERTSSLLPRVQKKEEPSVAPSEPKTDLD
                     RFCNPDDRKPCITVCSPASPCECDCESVRRSLVRELEERRIPRTVGRAKTGCGGSCKS
                     GPFIGFPQRGFFYLGVRPEDVPEIVRETVLGGKILFPFLSVNPDRAYRSDILFEKETG
                     MLAAIHEGVCMVETAKYFLDFDEGLSCGKCAPCRIGMKRMQEIMERVVSGNGTLPDME
                     QIRILCATMRETPHCEFAVTSSRPVLSALTYFEDEFMAHIEGKECPAGVCRELVELQR
                     KKAVRQRLTAKKR"
     gene            complement(2613..4034)
                     /locus_tag="PLM79_11435"
     CDS             complement(2613..4034)
                     /locus_tag="PLM79_11435"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HOV86968.1"
                     /translation="MMKWVLFIPELYYVVMAAVFFGWSIHRKGEPRRLFGWALVLSAV
                     GVVVTLGSVQLEGMLFFDAYRVDFFSQVFKFLLALGTFWVIFTCSELNGIEERQHAEF
                     YFFLSVCTLAMMMLVSSVELLTIYVSIELSSYSLYLLVPMRRQVDEYVETGIKYFMVG
                     VTASAFMLFGISLLFGVTHTTYVGKILQVLPGLIREPAAVVGLVLALSGFFFKQALFP
                     FHIWAPDVYQGAANQVTTYIATASKMAAAAMVIRFVGFSGGESPGFVYLLIGLSVVSM
                     TFGNLVAIIQKDIKRLLAYSSIAHAGYMLIGILSMTQRGYASAIYYAVAYLVMTFACF
                     MVILKVADDGHNLQIKELAGLHRRSPLLAMTLMMGLFGLAGIPPTVGFTGKFLVFAAA
                     LEKGYLWLVIVGMINATISLYYYLIVIKAAYLLEPLKPYPGVRVDPWTRALSIVLVIL
                     MVYLGIFPDQFVALTEAAARSLL"
     gene            complement(4062..5537)
                     /locus_tag="PLM79_11440"
     CDS             complement(4062..5537)
                     /locus_tag="PLM79_11440"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOV86969.1"
                     /translation="MTPMETAPFPILSTILLSTIVGLLVILFIPEDRPKLVKQVSLLF
                     AGVGLVLCLWLYYAYDVQKGGFQFVERFLWVKSLGIYYFNAVDGINLPMLLLTAVVLF
                     TGVWTMWELEVRVKEFFAFMYLLVAGVYGVFMSMDLFWIFVWYDVSLFPMYPLIAIWG
                     GTRKEYGGMKLTLYLLAGSALILPGILYLWVQGGAQTFDLLELQRIGFPLGVQKSAFV
                     MLYLGFGILAAAWPFHTWSPVGHVAAPTAVSMIHAGVLMKLGAYGILRLGMTLCPEGL
                     AYWADFMAVLATVGIVYGAFVGLAQTDLKYVIGYSSVSHMGMVGLGLSTMTVNGINGA
                     VFQMFAHGIMTALFFSAVGYIYDKTHTKMIPELGGLSKTMPMASAFFIVAALAGVGVP
                     CMASFWAELLVFVSAVKTYPVRGILAVLALVVGALFMLRVVQKTFYGEKNERWAHIPD
                     VPPFLMTPRIILIGFILFFGLFPKMLLDVIQSAVVPFVAGL"
     gene            complement(5669..7615)
                     /locus_tag="PLM79_11445"
     CDS             complement(5669..7615)
                     /locus_tag="PLM79_11445"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOV86970.1"
                     /translation="MAWIFKDSVLLAAALATCLPFFSMAIILLFTRKNPRLSCTISIS
                     AVTVSLGCALYLLRTLWDARVPLEYRVSWLAADNLVVPYGYLLDPLSLLMLTIVAVIS
                     FLVQVYSVGYMAGDPGYSRYYAFQSLFAWAMMSMVVAPSMLQLFIFWELVGLASYFLI
                     GFYYEKWSASQAGKKAFVMTRLGDVGFFLGLIILFLGYGNLGITDINEAARANAISPY
                     LASIAAFLIFCGVVGKSAQFPLITWLPDAMEGPTPVSALLHSATMVAAGVYMFSRIFP
                     FFAVSPDAMSVALAVGTVTMLLASTMAMATYDLKQVWAYSTVSQLGFMVMALGAGGYF
                     AGVFHLTTHAGFKALLFLCSGIFIHEYHTNDMRIIGRRGGRKRGIPMVCMTVGALALS
                     GVFPMAGFFSKEAILGVLAGHPNKIWLVAGLLGALMTAYYSFRLIFFLLFPKGGVEPP
                     SHGPNSHGHEPHEEEHRGHIFWTMAWPVMILAGVTLVLGFCQSSLESFLMGAGTHGAV
                     PAAHDAAGEHGAHGAGHYVLLAAAVGLALAGIAWAWVEFGRKKASREGFLARLPRTRE
                     LFGRRWYMDDFFRKFLDRVVYAGFTSLFTRNDRRVIDGAIDGICAGTVGGGRLFSFLQ
                     SGMLQYNLLLMITAVGIVVLLFLL"
     gene            complement(7622..7930)
                     /gene="nuoK"
                     /locus_tag="PLM79_11450"
     CDS             complement(7622..7930)
                     /gene="nuoK"
                     /locus_tag="PLM79_11450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOV86971.1"
                     /translation="MNTLETYLVVAAVLFCLGLLGVLQRRNLIGILISIELMLNGANI
                     NFMAFNRFLAPEPAVGQIIALFVMGLAAAEAAIGLSIILALFRRLHSINIERAQRLRG
                     "
     gene            complement(7930..8451)
                     /locus_tag="PLM79_11455"
     CDS             complement(7930..8451)
                     /locus_tag="PLM79_11455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOV86972.1"
                     /translation="MSFPGVENLAQMAFWVTLGFTLTGAFIAVFPRNILYNVLGLSLC
                     LAGIGGIYLCLGSFFIALMQWLIYIGAITIAIVFAIMLSRPLYTAVPERKVPKVALAT
                     AAGAVFLVAVLGVIFKTNWKPAVERGKDWSVAAIGHLLLTRYDLVFEVISLVLLVAII
                     GAIVTAGYSRRLS"
     gene            complement(8478..8927)
                     /locus_tag="PLM79_11460"
     CDS             complement(8478..8927)
                     /locus_tag="PLM79_11460"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HOV86973.1"
                     /translation="MGYVKDILIGGNSLIQGMKVTARRLFRPVVTVQYPREELELSPA
                     YRGHIELRRFKETGTHRCIACGTCQRTCPSNVIKVQGVKERAKSPKTATHYVIDFTRC
                     SLCGLCVECCPTQTLRYSMEYQLEGGSRWDGVIDLMARLEKTASQGS"
     gene            complement(9072..10052)
                     /gene="nuoH"
                     /locus_tag="PLM79_11465"
     CDS             complement(9072..10052)
                     /gene="nuoH"
                     /locus_tag="PLM79_11465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOV86974.1"
                     /translation="MGTETGFELIRVVIGLVLVLAFSQLNALFLVWLERKVAGHIQLR
                     LGPMEVGPHGLLQTIVDGIKLVGKELIVPLAADKKLFVIAPILVFMPALIGFLVLPFG
                     PDLIIRDMNVGVLLVFSFSTFTVLAILAGGWASNNKYALLGAVRSVAQNVAYEIPLLL
                     AAMSVVMMVNSLRFSEIVEAQNRIWFIFVQPVAGLIYLICATAETNRAPFDIPEAESE
                     LVAGFHTEYSGMRFGLFFIAEYTNMFVVSAVATSLFFGGWHGPFGFGLGIPGIVWFLI
                     KTYILIFVVMWVRWTFPRVRFDQLMNFSWKFMIPLSLANLVVTAMVLKLL"
     gene            complement(10072..11193)
                     /locus_tag="PLM79_11470"
     CDS             complement(10072..11193)
                     /locus_tag="PLM79_11470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOV86975.1"
                     /translation="MAEQFRKPVVDQIYTLNLGPQHPSTHGVLRMLLDLDGEYIVKAD
                     PTIGYGHRGHEKMGENRTYKQFMPNTSRMDYLSGMLYNHGFALAVEKLAGIEVPARAR
                     YIRVICAELNRISSHLLWFGTYVMDLGGFTPFLYAFDDRERILDLLDRVSGSRLTYSY
                     CRFGGVARDVDSGFVEGTRSFLQRLRSRFPDYHSLVTENVIFIYRTRGVGVVPAELAR
                     QYGVTGPNLRACGIAYDVRKVEPYEVYDRFEFEVPVGVNGDALDRYNVRLAEMEQSCR
                     IIEQALDQLPPGPYLNEKAPFRVRPPKGEVYQAFESARGLTAYYLVSDGSACPYRCHI
                     RVPSFGNLHVLEEVLKGTLVADAISILGSIDVVVPEIDR"
     gene            complement(11186..11659)
                     /locus_tag="PLM79_11475"
     CDS             complement(11186..11659)
                     /locus_tag="PLM79_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOV86976.1"
                     /translation="MARLNEVLEKLRQILPADTLSFRDPSRTGTSCCARVGLDRVREV
                     ASIFARGGFFLESLTGLDFQDSKELVYHFNTYEPGARVAVRVLCPHGEAAPTLSDLFR
                     SASWQEREVWEFFGIPFAGHPDLRPLLLPEDADFHPLTKTFGVVNAYRKREEIYG"
     gene            complement(11647..12186)
                     /gene="nuoB"
                     /locus_tag="PLM79_11480"
     CDS             complement(11647..12186)
                     /gene="nuoB"
                     /locus_tag="PLM79_11480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HOV86977.1"
                     /translation="MGTKKLGSGEPVIRFALLDEVLNLARANSLWPMTFGLACCAIEM
                     MAAGASRFDLDRFGAGVFRPSPRQSDLMIVAGTISKKMASAIQMLWDQMPNPKWCMAM
                     GNCAISGGPFEYEGQYAIVPGADLIVPVDVYVPGCPPRPEGLIQGFLALEDKITGGEN
                     RNLLRRFHRLGGKKKSWLG"
     gene            complement(12168..12548)
                     /locus_tag="PLM79_11485"
     CDS             complement(12168..12548)
                     /locus_tag="PLM79_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOV86978.1"
                     /translation="MLSKELMTDFLSVGLFLLAGLVFAVIPILVSSLIVRRSKGPQRD
                     ETYESGMHTIGSAWIQFTVAYYIYALIFLAFDVDVLYLFPVALVYGQYALRDFVEVII
                     FVGILSLAIVYAWRKGVFEWERRS"
     gene            complement(12563..14416)
                     /locus_tag="PLM79_11490"
     CDS             complement(12563..14416)
                     /locus_tag="PLM79_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV86979.1"
                     /translation="MNRLSSVQDLEVLRREILARQSKMLKWIRVCCGPACHSRGGEKL
                     ARAFQRYVEENNLSDRYLVKPTGCNGFCAEGPTVVIEPDNLFYGHVGIERVPEIMERT
                     VIGGELIEDLLYVDRETGSRVLREREIPFYAKQLRLVMEDTAANHTTEIDDYISRGGY
                     SALARALSEMTPEQVIAEVKGAGLRGRGGGGFPAGVKWESCRRARGEIKYVMCNADEG
                     DPGAYMDRAVLEGNPHVVIEGMIIAAYAIGAGEGYIYCRYEYPLAVKNGNHALDRARA
                     YGLLGKNILGTGFDFDIYLNRGAGAFICGESTALMASLEGKIGEPRGKHIHTVEKGLW
                     NRPSNLNNVETYANVPQIINRGGKWYASIGTERSKGTKIFSLVGKINNTGLVEVAMGT
                     PLRKIVYEIGGGIPQGKRLKAVQTGGPSGGCIPEELIDLPVDYEKLKEAGSMMGSGGM
                     IVMDEETCMVDVARYFLNFLTEESCGKCVPCRVGVKRMHEIVTAICEGRGTPADVDQL
                     EKMGRTLQEASLCGLGKSAPNPVLSTIRYFRHEYEAHVRDRKCPAKVCKPLLTYEIDT
                     EKCTGCGRCRIKCPSGAVIGAKKECHCIDEELCVRCGICIESCKFGAITIE"
     gene            complement(14427..14900)
                     /locus_tag="PLM79_11495"
     CDS             complement(14427..14900)
                     /locus_tag="PLM79_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV86980.1"
                     /translation="MELNLTDVDAIIDHWGPKPDSLLQIMLDINHRFRFLPRESMERV
                     AERLRMPVSQVFGVANFFKVFRLKPQGRVLVHVCTGTACHVKGTPRLLDRIQQDLHLR
                     PGDTTEDLSLTLETVNCVGACASAPVVVVDGVTYGQATPTEISQLAKKLMETNKA"
     gene            complement(15037..>16160)
                     /locus_tag="PLM79_11500"
     CDS             complement(15037..>16160)
                     /locus_tag="PLM79_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOV86981.1"
                     /translation="GTVGPSARTKVDPPKILVIDDEEIARISCERVLKREGIRVTLAS
                     GGREGLDVLMRDPHDLVLVDLKMPEMDGMEVTRRIRALDPNIVAIIITGYATIESAVA
                     VMKEGAYDYLPKPFTPDELLIVVRRGLEKRRLDRESRALREEKEAMERNFITMVTHQL
                     RSPLAAIYQYLEVLLSDLGGELGEMQREMIERARERLEGLLELINDWLDMSRIRAGEI
                     VDRLRAVALRTCVDAALAGLELSARERGVSVSTAIPPDLPEVMVDPESFREALSNVIS
                     NAVKYNREGGRIWIRAMERDGWVVVEVEDTGYGIREAEIPFIFEQFYRSKASEIRRQQ
                     GTGLGLSIIRKILQAHGGKVEVRSELGKGTTFSLFINKA"
BASE COUNT         3357 a         5030 c         4819 g         2954 t
ORIGIN      
        1 ttcgttgtcg ggagaggtgc cggggtcgat cccgtagacc aggcacgcgg gcttcctgcc
       61 cgcaaacagc cgggcggctt tcaggatcct ttcctcggga attcccgtct gtgcggccac
      121 cgaagacagg ttgatcttct cgaggctctt ctccagtttc tgaaaatcct gggtgtgggc
      181 ggcaacgtag ttgcggtcgt gcaatccttc ggccacgacg gttttcagga tccctgcaag
      241 gagggcccct tcggagccgt tcttcatggg caggtgcagc gtggccgtct tggtcaggag
      301 gaccttttcg ggatgggcca cgaggagcac cgccccttcc cgggccgcct ttcgagcccg
      361 aagagccgca atggcgtgct tttcgagggt gtccgacccg agtaccagga cggctcccgc
      421 ccgggcaatg tccgagaggg gattggtcga ggctccgaac cccaaaaccc ccgaaagccc
      481 gtccgccatg gcttgctgcc acggtccccc gacgctgtcg acgtgattgg tcgccagggc
      541 tccccgggcc agtttttggg ccaggtaata ttcttcgttg gtgagagatc ccccgcacaa
      601 gaccgcaagg gagttttccc ccctctggtc ccggacgcgc ttgagtcctt gggcggcggc
      661 gcgaaggccc tcttccacgg aaactgcttc cggtgttccc gactcgttgc gcacgaaagc
      721 cgtggtgagc ctttcgggag aattcatgaa gtcgaggttg aagcgtcccc ttttgcacaa
      781 ggctcccgag tttgccgggg agttgaaatc ccccaggacc cgtaccacct tcccgtggaa
      841 ggtgtcgtac tcggcccggc accccgatgc gcactggagg cacacggtgg aactccttcg
      901 atactgccag gcccgcacgg ttccccgccc ggcgcggaag ccgatggcgt ccacggggca
      961 gaaatcggcg caatgaccgc aggattcaca gcccgcctcg tccaggggca gatcgaacgc
     1021 cgtcgagatc cgctgttcga acccccgccc caccttggtc cagatgtcgg ccccctcgac
     1081 gtcgtggcac gcccgaatgc accggcgaca caggatgcac ttgttcatgt caagatgaag
     1141 aacgggattg gggtccggat ccacgggatg gctctgccgc gctccccgga acaccgagtc
     1201 cttcacccgg taccggtggg ccatgtcctg gagctcgcat tcccccgtgg cgtaacaggt
     1261 catgcaatcg ttggggtgat tggacaggag cagctccatg atggtccgcc gcgcatcgag
     1321 gaccttttcg gaatccgtct cgatgaccat tccgttgcgc acgggataca cacaggccgc
     1381 cacctggctg gaccagcctc cccggatctc ctcgaccacg cagattcgac aggcctcctc
     1441 caggggcaag aagggatggt aacacagggt tgggacgtag atccccgcct tcttggcggc
     1501 ctgaagaatg gtggacccct gttcgatttc aacttcccgg ccattgatcg taagcttcac
     1561 cgtcgccata gtactttttc cctttaggtt gctttcgcca tgttgggatc ctaagaagtg
     1621 gttgctctcc tttggccctg ttacctcttt tttgccgtga gcctctggcg aacagctttt
     1681 ttcctctgca actccaccag ctccctgcac accccggccg gacactcttt gccctcgata
     1741 tgcgccatga attcgtcctc gaagtaggtc aacgcactga gcaccggccg actgctcgtg
     1801 accgcaaact cgcaatgggg agtctcccgc atggtggcgc agagaattcg aatctgttcc
     1861 atgtcgggga gcgtgccgtt tccggaaacc acccgttcca tgatttcctg cattcgtttc
     1921 attccgatcc tgcagggtgc gcacttgccg caggaaaggc cttcgtcgaa atccaggaag
     1981 tacttggccg tctccaccat gcagacccct tcgtgaatcg ccgcgagcat cccggtttct
     2041 ttctcgaaga ggatgtcgct ccggtaggct cgatccgggt tgacggaaag gaagggaaaa
     2101 aggatcttcc cgcccagaac ggtctctcga acgatttccg ggacatcctc gggacgcact
     2161 cccaggtaaa agaaaccccg ttggggaaac ccgatgaagg gtccggattt gcaggacccg
     2221 ccgcacccgg tcttggccct gcccacggtc cgagggattc gccgctcctc gagttcccgg
     2281 acgagggacc ggcgtacgga ttcgcagtca cactcgcagg ggctcgcagg ggagcaaacg
     2341 gtgatgcagg gcttgcggtc gtcgggattg cagaaccgat cgaggtccgt cttcggctcc
     2401 gacggggcga cggagggttc ctccttcttc tgcacccggg gcagcaggga ggaggtcctc
     2461 tctcgtatca cccgccccaa atctttgaga ctatccaacg aagctctcat gcatttcctt
     2521 ttcttcggat cttcccgggc gggctttttc cggaacccgc gattcgaggc accgggtccg
     2581 gggacgaatc ccccccgccc tccccgggga agctagagca ggctgcgcgc cgccgcctcg
     2641 gtcaaggcga cgaactggtc cggaaagatt cccaggtata ccatcaagat caccagcacg
     2701 atgctcaggg cacgggtcca gggatccaca cggacccccg ggtacggttt caagggttcc
     2761 aggagatagg ccgccttaat gacaatgagg tagtagtaga gggaaatggt ggcattgatc
     2821 atgcccacga tgacgagcca caggtagccc ttttccaggg ctgcggcaaa gaccaggaac
     2881 tttcccgtaa atcccaccgt gggcggaatc cccgccagcc cgaagagccc catcatgagg
     2941 gtcatggcca ggagggggga acgccggtgc aggcccgcca gttccttgat ctgcaggttg
     3001 tggccgtcat cggcaacctt gaggatgacc atgaagcatg caaaggtcat taccaggtac
     3061 gcgacggcgt agtagatggc cgaggcgtag cccctctggg tcatggaaag gatcccgatg
     3121 agcatgtacc ccgcatgggc aatgctcgag taggccagaa gcctcttgat gtccttctga
     3181 atgatggcca ccaggttgcc gaaggtcatg gagacgacgg aaaggccgat gagcaggtag
     3241 acaaaaccgg gactttcccc gccggagaat cctacgaatc ggatgaccat ggcggcggcg
     3301 gccatcttcg aggccgtggc gatgtaggtg gtcacctggt tggctgcgcc ctggtacaca
     3361 tccggggccc agatgtggaa gggaaagagg gcctgcttga aaaagaaacc cgagagggcc
     3421 aggaccagcc ccaccacggc tgcgggttcc cggatgagac ccggcagcac ctggaggatc
     3481 ttgcccacat aggtggtgtg ggtcacgccg aagagcaggc tgatgccgaa gagcatgaag
     3541 gccgaagccg tcacccccac catgaagtac ttgatgcccg tctcgacgta ttcgtccacc
     3601 tggcgccgca tgggtacgag caggtacagg gaataggagg aaagctcgat ggatacgtaa
     3661 atggtgagca gttccacgga actgaccagc atcatcatgg ccagggtgca gacggaaagg
     3721 aagaagtaga actccgcgtg ctgcctctcc tcgatgccgt tgagctcgct gcaggtgaag
     3781 atcacccaga acgtccccag ggccagcagg aatttgaaga cctggctgaa gaaatccacc
     3841 cggtaggcat cgaaaaagag catgccttcg agttggacgg acccgagagt gacgacgacc
     3901 cccacggccg acaggaccag ggcccagcca aacaggcgtc tcggttcccc tttccggtga
     3961 atggaccaac cgaagaagac cgctgccatg accacgtagt acaattcagg gataaagagg
     4021 acccatttca tcatcacgaa tcattccccc ccactcggcg ctcatagacc ggccacaaac
     4081 gggaccacgg cgctctggat cacgtccagg agcatcttgg gaaacagccc gaaaaacagg
     4141 ataaacccga tgagaatgat gcggggcgtc atcaggaacg gcggcacatc cgggatgtgc
     4201 gcccaccgct cattcttttc accgtagaaa gtcttctgga ccacccgaag catgaacagg
     4261 gctcctacca ccagggccag aacggccagg attcctcgga cgggataggt cttcaccgca
     4321 gagacgaaga ccaggagctc cgcccagaag ctggccatgc agggaacgcc gaccccggcg
     4381 agggccgcca cgataaagaa cgccgatgcc atgggcatgg tcttggacag tccccccaat
     4441 tccgggatca ttttggtgtg ggtcttgtcg tagatgtatc cgacggcgga gaagaagaga
     4501 gccgtcatga tgccgtgggc gaacatctgg aagaccgcgc cgttgattcc gttcaccgtc
     4561 atggtggaca gccccagccc caccattccc atgtgggaga cgctcgagta gccgatgacg
     4621 tacttcaaat ccgtctgggc cagtcccaca aaggcgccgt agacgatccc cacggtggcg
     4681 agaacggcca tgaagtccgc ccaataggcg aggccttccg ggcagagggt catgcccagg
     4741 cgcaggattc cgtaggctcc cagcttcatg aggacccccg cgtggatcat gctcaccgcc
     4801 gtcggggccg ccacgtggcc cacgggagac caggtgtgga acggccaggc cgcggccagg
     4861 attccgaacc ccaggtagag catcacgaag gccgacttct ggaccccgag ggggaacccg
     4921 attcgctgca gttcgagaag atcgaaggtc tgggcccctc cctggaccca taggtagaga
     4981 attcccggca agatcagggc gctgccggcc agcaggtaca gggtgagctt catccctccg
     5041 tattctttgc gggtgcctcc ccagatggcg atgagggggt acatgggaaa cagggagacg
     5101 tcgtaccaga cgaaaatcca gaagaggtcc atggacatga agaccccgta gaccccggca
     5161 acgagcaggt acatgaaggc aaaaaattcc ttcacccgaa cttccagctc ccacatggtc
     5221 cacacgccgg tgaacagaac cacggccgtc aggagcaaca tgggaagatt gatgccgtcc
     5281 acggcattga aatagtagat ccccaggctc ttgacccaca ggaaccgctc gacgaactgg
     5341 aacccgccct tctgcacgtc gtaggcgtag tagagccaga ggcacaagac caggcccact
     5401 cccgcgaaca acaggctgac ctgcttgacc agcttgggtc gatcctcggg aatgaacagg
     5461 atgaccagca acccgacaat cgtggaaagc aggattgtcg acaagatggg gaaaggagcc
     5521 gtttccattg gcgtcatagg acatccaccg atcccaaaag ttctcagtta tgcttctgcc
     5581 gcacccgggg gcagaactcg attcgcctgc ccgccgtctt tccccgccag cccccgagga
     5641 ccggggaccc tgcgggcaca aaccgtccct agaggaggaa gagcaagacc acgatgccca
     5701 cagcggtgat catcagtaga aggttgtact ggagcattcc cgattggaga aagctgaaga
     5761 gtcgcccgcc tccgacggtc ccggcgcaga tgccgtcgat ggccccgtcg atcacccgcc
     5821 ggtcgttgcg ggtaaaaagg ctggtgaagc cggcatacac cacccggtcc aggaatttcc
     5881 ggaagaagtc gtccatgtac caccgtcggc cgaaaagttc ccgggttcgc ggcaaccggg
     5941 caaggaagcc ttcccgggaa gctttcttcc gaccgaactc cacccaggcc caggcaatcc
     6001 ctgcgagtgc caacccgacg gcggccgcca gcagcacgta gtgacccgct ccgtgagccc
     6061 cgtgttctcc tgcggcgtca tgagcggcgg gaaccgcccc gtgagtcccg gctcccatga
     6121 gaaagctctc cagggacgac tggcagaacc ccaggaccag ggtgactccc gccaggatca
     6181 tcaccggcca ggccatggtc caaaagatat ggccgcggtg ttcttcctcg tggggttcgt
     6241 gaccgtgaga attcggcccg tgggaggggg gttcgacgcc ccctttgggg aagagcagga
     6301 agaagatgag ccgaaagctg tagtaggccg tcatcagggc ccccagcagg cccgccacga
     6361 gccaaatttt gttgggatga ccggccagga ctcccaggat cgcttccttg ctgaaaaagc
     6421 ccgccatggg gaaaaccccg gacagggcca gggcccccac cgtcatgcag accatgggaa
     6481 tcccccgttt ccggcctccc cggcggccga tgatgcgcat gtcgttggtg tggtattcgt
     6541 ggatgaaaat cccggagcac aggaacagca gggccttaaa ccccgcgtgg gtggtcaggt
     6601 gaaacacccc ggcaaagtag ccccccgcac ccagggccat gaccatgaac cccagttggc
     6661 tcacggtgga ataggcccat acctgtttca agtcgtaggt cgccatggcc atggtggacg
     6721 caagaagcat ggtgacggtg ccgacggcca gcgccacgct catggcatcg ggggaaacgg
     6781 caaagaaggg aaagatcctg ctgaacatgt acacgcccgc cgcaaccatg gtggccgaat
     6841 ggagaagggc gctcacgggg gtcggacctt ccatggcgtc gggcaaccag gtgatgaggg
     6901 ggaactgggc gctcttgccg acgacgccgc aaaagatcag gaaggcggcg atacttgcca
     6961 ggtacggaga gatggcgttg gcccgggccg cttcgttgat gtcggtgatg cccaggtttc
     7021 cgtacccgag aaacaggatg atgagcccca gaaagaagcc cacgtctccc aggcgggtca
     7081 tgacgaaggc cttcttccca gcctgagaag cgctccattt ctcgtagtag aacccgatga
     7141 gaaaatagga agccaggccc accagttccc agaaaataaa cagctgaagc atgctggggg
     7201 ccaccaccat gctcatcatg gcccaggcaa acagggactg aaacgcatag taacggctat
     7261 aacccgggtc tccggccatg tagcccacgg agtacacctg gacgaggaag ctgatgacgg
     7321 ccacgatggt gagcatcaac aggctgaggg gatccagcag gtagccgtaa ggaacaacca
     7381 ggttgtcggc cgcaagccac gaaacccggt actcgagggg gacccgggca tcccacaggg
     7441 tgcggagcag atacagggcg cacccgaggg agaccgtcac cgccgagatg gaaatggtgc
     7501 acgacaggcg ggggttcttc cgggtgaaaa gaaggatgat tgccatggaa aagaaaggca
     7561 ggcacgtggc aagagcggct gccaacagga ccgaatcttt gaatatccag gccataggat
     7621 cttaacccct caatctctgt gcgcgctcaa tgttgatgga atgcagccgt cggaacagcg
     7681 ccaggatgat gctcaaccca atggcggcct cggccgcggc caatcccatc acgaacaggg
     7741 cgatgatctg ccccaccgcg ggctcgggtg ccaggaaccg gttgaacgcc atgaagttta
     7801 tgttggcccc gttgagcatc agttcgatgg agatgaggat cccgatgagg ttccgtcgtt
     7861 ggaggacccc caggagcccc aggcaaaaca ggacggcggc aacaaccagg tatgtttcaa
     7921 gggtattcac tagctcaacc tcctcgagta tccggccgtc acgatggctc cgatgatggc
     7981 cacaaggagc accagggaaa tgacttcgaa gaccaggtcg tagcgggtca gcaaaagatg
     8041 cccgatggcc gccaccgacc agtctttgcc tctttccacc gcgggcttcc agttcgtctt
     8101 gaagatcact cccaggaccg ccaccaggaa gaccgcaccc gccgccgtag ccagggccac
     8161 cttcgggacc ttcctttcgg gaaccgccgt atagagcgga cgcgacagca tgatggcaaa
     8221 cacgatggcg atggtgatgg ccccgatgta gatcagccac tgcatcaagg cgatgaagaa
     8281 gctgcccagg cataggtaga tcccgccgat tcccgcaagg cacaaagaca atcccagaac
     8341 gttgtataga atgttcctgg gaaagaccgc gatgaaggct cccgtaaggg tgaatcccaa
     8401 ggtaacccag aaggccattt gcgccagatt ctccacgccc ggaaaactca tgccctcttc
     8461 ctttctcccc tatgggtcta cgagccctga gaggccgtct tttcgagtct cgccatgagg
     8521 tctatgacgc cgtcccaccg ggacccgccc tccagttgat actccatcga gtaccggagg
     8581 gtttgagtgg ggcagcattc gacgcagagc ccgcagaggc tgcagcgggt aaagtcgatg
     8641 acataatggg tggccgtctt gggactcttg gcccgctcct tgaccccctg gactttgatg
     8701 acgttggacg ggcacgtcct ctggcacgtc ccgcaggcga tgcagcggtg ggtcccggtt
     8761 tccttgaacc gcctgagctc gatgtggccg cggtaggcag gggagagctc gagctcctct
     8821 ctcggatact gcaccgtcac aacgggccga aagagccggc gagccgtgac cttcattccc
     8881 tggatgaggc tgtttccacc gatcagaata tccttgacgt atcccatacg gcctctttcc
     8941 tgtcgttcat gaatacacac accggccaac gccgggggaa ggcctccggt gcggggcgac
     9001 cggaaacccg cgaaccgcat ctcccttctc cggggtgcct cccgcggcaa gcccaaaacc
     9061 ggctcgtccc gctacagcag cttcaacacc atcgccgtca ccacgagatt ggccaggctc
     9121 agcgggatca tgaacttcca tgagaaattc atgagctggt cgaaccgaac ccgggggaac
     9181 gtccagcgga cccacatgac cacaaagatg aggatgtagg tcttgatcaa aaaccaaacg
     9241 attcccggaa tccccaggcc aaacccgaag gggccgtgcc acccgccgaa gaagaggctt
     9301 gtggccacgg cgctcaccac gaacatgttg gtgtattccg caatgaaaaa gagcccgaac
     9361 ctcataccgc tgtattccgt gtgaaacccg gcaaccagtt ccgattcggc ctcggggatg
     9421 tcaaaggggg cccggttggt ctccgccgtg gcgcagatga ggtaaatgag ccccgctaca
     9481 ggctgcacaa agatgaacca gatccggttc tgggcctcga cgatctcgga gaaccggagg
     9541 gaattgacca tcatcaccac gctcatggcc gccaggagca gagggatctc gtaggccacg
     9601 ttctgggcca cggaacggac ggcccccagg agggcgtact tgttgttgga agcccagccg
     9661 cccgccagga tggcaagaac ggtgaacgtc gagaaagaaa agaccagcag gaccccgacg
     9721 ttcatgtccc gaatgatgag atcgggcccg aagggcagga ccagaaaacc gatgagggcg
     9781 ggcatgaaca cgaggatcgg ggcaatgacg aagagttttt tgtcggcggc aagagggacg
     9841 atcagttcct tgcccaccag cttgattccg tccacgatgg tctgcaacag cccgtggggt
     9901 cccacctcca tgggccccag gcgaagctgg atatgcccgg ccacttttcg ttcgagccaa
     9961 accaggaaaa gggcgtttag ctgggaaaag gccaggacca ggaccagccc gatcaccacc
    10021 cggatcaact cgaagcctgt ttcagttccc atttcggcag cgtcctctca cctaacggtc
    10081 tatttccgga acgaccacgt cgatacttcc caggatcgag atggcatcgg ccaccagggt
    10141 gcccttgagc acctcctcga gcacatgaag gttcccgaag ctgggcaccc gaatgtgaca
    10201 gcgatacggg caggcactgc cgtcactcac caggtagtag gccgtgaggc cccgggcgct
    10261 ctcgaaggcc tgatacacct cgcccttggg ggggcgaacc cgaaacggcg ccttctcgtt
    10321 caggtacggg cccgggggga gctggtccag ggcctgctcg atgatccggc aactctgctc
    10381 catttccgcc aggcgcacgt tgtaccggtc cagagcgtct ccgttcaccc cgacggggac
    10441 ttcgaactcg aaccggtcgt acacctcgta gggctcgacc ttcctcacat cgtaggcaat
    10501 cccgcaggca cggaggttgg gccccgtaac cccgtattgc ctcgccagct cggcagggac
    10561 gacccccacc ccccgggtgc ggtagataaa aatcacgttc tcggtaacca ggctgtgata
    10621 atcgggaaat cggctccgta gccgctggag gaaggaacgg gtgccctcca cgaaccccga
    10681 gtccacatcc cgggcgaccc ctccaaaccg gcaataggaa taggtcagcc gggacccgct
    10741 cacccggtcc aggaggtcca ggatccgttc ccgatcatcg aaggcgtaga ggaagggagt
    10801 gaagcccccc aggtccatga cgtaggtccc aaaccagagg agatggctcg agattcggtt
    10861 gagctcggca cagatgaccc ggatgtagcg cgcccgggca gggacttcga tccccgccag
    10921 cttctccacc gccagggcaa acccgtggtt gtagagcatc cccgacaggt agtccatgcg
    10981 gctggtgttg ggcatgaact gcttgtaggt ccggttttcc cccattttct cgtggcctcg
    11041 atggccgtag ccgatggtgg ggtccgcctt gacgatgtac tcgccgtcca ggtccaggag
    11101 catcctcaac acgccgtggg tgctggggtg ctggggcccc aggttcagcg tgtagatctg
    11161 gtcgacaacg ggttttcgaa attgctcagc catagatctc ttctcgtttt cgataggcgt
    11221 tcacaacccc aaaggtcttg gtcagcgggt gaaaatcggc atcctcgggc aggaggagcg
    11281 gacgaaggtc cgggtgcccg gcaaaaggaa tgccgaaaaa ctcccagact tccctctcct
    11341 gccaggaggc ggaacggaaa agatcgctca gggtgggcgc cgcctcccca tgggggcaaa
    11401 gcacccgtac ggccacccgg gcccccggct cgtaggtgtt gaaatggtag accagttcct
    11461 tggaatcctg aaaatccaac ccggtcaagg attccaggaa gaatcccccc cgagcgaaaa
    11521 tcgaggccac ctcgcggacc cgatcgagtc ccacccgggc acaacagctc gtgccggttc
    11581 gggaaggatc ccggaacgaa agggtgtcgg cgggaaggat ttggcgaagt ttttccagta
    11641 cctcgttcag ccgagccatg atttcttctt tcctcccagg cggtgaaaac gtcgcaggag
    11701 gttgcggttt tccccgccgg tgatcttgtc ttccagggcc aggaacccct ggatgagacc
    11761 ctccggccgg gggggacatc cggggacgta aacgtctacg ggcacgatga ggtcggctcc
    11821 gggaacaatg gcgtactggc cttcgtattc gaagggcccc ccggaaatgg cgcaattgcc
    11881 catggccatg caccacttgg ggttgggcat ctggtcccag agcatctgaa tggccgaagc
    11941 catcttcttg gaaatggtcc cggccacgat catcaggtcc gactggcgtg gggaaggacg
    12001 aaagaccccc gccccgaaac ggtccaggtc gaaccgggat gctcccgccg ccatcatctc
    12061 gatggcacag caggcaagac cgaaggtcat gggccacagg gaattggccc tggccaggtt
    12121 cagcacctcg tccaggagag cgaatcggat caccggctcc ccggagccta gcttcttcgt
    12181 tcccactcga agactccttt tctccaggcg tacacaatgg ccagggagag aattcccaca
    12241 aagatgatca cttccacgaa atcccgcagg gcgtactggc cgtacaccag ggccacgggg
    12301 aacaggtaga gcacgtccac gtcgaaggcc agaaagatca aggcgtagat gtaataggcc
    12361 accgtaaact gaatccatgc gctcccgatg gtgtgcatgc cgctctcgta ggtctcatcc
    12421 cgctggggac ccttgctgcg ccgaacgatg agggaggaga ccaggatggg gatcacggca
    12481 aaaacgagac ccgccaggag gaacagcccc acggacaaaa aatcggtcat gagttctttg
    12541 gaaagcattc catgctccga ttctactcga tggtaatggc tccgaattta cagctctcga
    12601 tgcagattcc gcatcgcacg cagagttcct cgtcgatgca gtggcattcc ttcttggcgc
    12661 cgatgacggc tcccgaggga cacttgatcc ggcaacgccc gcacccggtg cacttttccg
    12721 tgtcgatttc ataggtgagc agaggcttgc acacctttgc cgggcatttg cggtcccgca
    12781 cgtgagcttc gtactcgtgg cgaaagtagc ggatggtgct cagaaccgga ttgggagcgc
    12841 ttttccccag tccgcacagg gaggcttcct ggagggtcct gcccatcttt tccaactggt
    12901 cgacgtcggc gggagtcccc cttccttcac agatggccgt cacgatttca tgcattctct
    12961 tgacgcccac ccgacaggga acgcacttgc cgcaggattc ctcggtgagg aaattcagaa
    13021 agtagcgggc aacgtccacc atgcaggttt cctcatccat gacgatcatg ccgcccgacc
    13081 ccatcatgga ccctgcctcc ttgagcttct cgtagtccac gggcaggtcg atgagctcct
    13141 cggggatgca tccccccgac ggtccccccg tctggacggc tttgagccgc tttccctggg
    13201 gaattccgcc cccgatttcg tagacgatct ttcgaagcgg ggtccccatg gccacttcca
    13261 cgaggcccgt gttgttgatt tttcccacca gggagaagat cttggtgccc ttgctgcgct
    13321 cggtcccgat ggaggcgtac cacttgcccc cccggttgat gatctgaggg acgttggcgt
    13381 aggtctcgac gttgttgaga ttgctgggac gattccagag ccccttttcc accgtgtgga
    13441 tgtgcttgcc gcgaggctcc ccgatctttc cctcgaggga agccatcagg gccgtggatt
    13501 ccccgcagat gaaggctccg gccccccgat tcaggtaaat gtcgaagtcg aacccggtgc
    13561 ccaggatgtt cttccccaaa agaccgtagg cccgggcgcg atcgagggca tggttcccgt
    13621 ttttcaccgc caggggatac tcgtaccggc agtagatgta gccttccccg gcgccgatgg
    13681 cgtaggcggc gatgatcatt ccctcgataa ccacgtgggg atttccctcg aggaccgcac
    13741 ggtccatgta ggctccgggg tccccctcgt cggcgttgca catgacgtac ttgatctccc
    13801 cccgggccct ccggcaggat tcccacttga ccccggcggg aaatcctccc cctccccttc
    13861 cccgcaggcc cgcccctttg acctcggcga tgacctgctc gggagtcatt tccgagaggg
    13921 ccctggccag ggccgagtag cctccccggg aaatatagtc gtcgatttcc gtagtatggt
    13981 tggccgccgt gtcctccatg accaggcgca attgctttgc ataaaaaggg atctcccgct
    14041 cacgaaggac ccggctcccc gtttcccggt ccacgtacag gaggtcttcg atcagctcgc
    14101 cgccgatcac cgttcgttcc atgatttccg ggacccgctc gatccccacg tgcccgtaga
    14161 aaagattgtc gggttcgatc accaccgtgg ggccttcggc gcaaaatccg ttgcatcccg
    14221 tcggcttcac caggtagcgg tcggaaaggt tgttttcctc cacgtagcgt tggaacgccc
    14281 gggcgagctt ttctcctccc cgggaatggc aggcggggcc gcaacacacc cggatccact
    14341 tcagcatctt gctttgccgg gcgaggatct cccggcgcag gacttccagg tcttgaacac
    14401 tgctcagtcg attcatagcc ggtttcctag gccttattgg tttccatcaa cttcttggcc
    14461 aactggctga tctcggtggg agtcgcctgc ccgtaggtga cgccgtcgac caccaccacg
    14521 ggagccgaag cgcaggctcc cacgcaattg acggtctcga gggtgaggga caggtcttcg
    14581 gtggtgtcgc cgggtcgcag gtggaggtcc tgttggattc ggtccaggag gcggggggtt
    14641 cctttgacgt ggcaggcggt ccctgtgcaa acatgtacaa ggacccgacc ctggggcttg
    14701 aggcgaaaga ccttgaagaa attggcgacg ccgaacacct gactgacggg catccggagc
    14761 cgctcggcca ctcgttccat gctttcccgg ggcagaaagc ggaaccggtg attgatgtcc
    14821 aacatgatct gaagaaggga atcgggtttg ggtccccaat ggtcgataat ggcgtccacg
    14881 tccgttaggt tcaactccat gaagagccct ccatccggcc gcccccgggg ggattgcgga
    14941 ccggcaaagc tggtttgcga cttgccctcc atacgtggca caatcgctgg gggtcgtcaa
    15001 gtgaaatatt gttcatagtg acccgcacaa aattctttag gccttattga tgaacaagga
    15061 aaaggtcgtt cccttgccca attcgctgcg gacctccacc tttcccccgt gggcctggag
    15121 aatcttgcgg atgatggaaa ggcccagtcc cgttccctgc tggcgccgaa tctcgctggc
    15181 cttgctccgg taaaactgtt cgaagatgaa cgggatctcc gcctcccgga tcccgtatcc
    15241 cgtgtcctcc acctccacca ccacccaacc gtcccgttcc atggcccgga tccaaattcg
    15301 gccgccttcc cggttgtact tgacggcatt ggagatgacg ttgctcaggg cttcccgaaa
    15361 ggattccggg tctaccatca cctcgggcag gtccgggggg atggccgtgg aaacgctcac
    15421 ccccctctcc cgtgccgaaa gctcgaggcc tgccagggcc gcatccacgc aggtccgcag
    15481 tgcaacggcc cggagccggt ccacgatttc cccggcccgg atgcggctca tgtccaacca
    15541 gtcgttgatg agctccaaca ggccctcgag ccgttcccgg gcacgctcga tcatctcccg
    15601 ctgcatctct cccaactctc cccccagatc gctgagcagc acctcgaggt actggtagat
    15661 tgcggccagg ggggatcgaa gctggtgggt gaccatggtg atgaaattgc gctccatggc
    15721 ctccttttcc tccctaagag cccgggattc ccggtccagc ctgcgcttct ccaggccccg
    15781 gcgcaccacg atgaggagct cgtcgggtgt gaagggcttg ggcaggtagt cgtaggcgcc
    15841 ctccttcatg accgccacgg cgctctcgat ggtggcatat cccgtgatga tgatggccac
    15901 gatgttggga tccagggcgc ggatcctccg agtaacctcc atgccgtcca tctccggcat
    15961 cttcaggtcc accaggacca ggtcgtgggg atccctcatg agcacgtcca ggccttcccg
    16021 acccccggac gccagggtca cccggatccc ctcgcgcttg agcacgcgtt cacacgagat
    16081 ccgcgcgatc tcttcgtcgt caatcaccaa gatcttggga ggatccactt ttgtccttgc
    16141 cgaggggcca accgtgcctc
//
LOCUS       DAOOVC010000087        16052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2444_length_16052_cov_6.602613, whole genome shotgun sequence.
ACCESSION   DAOOVC010000087 DAOOVC010000000
VERSION     DAOOVC010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16052
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2444_length_16052_cov_6.602613"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLM79_11505"
     CDS             complement(<1..935)
                     /locus_tag="PLM79_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOV86982.1"
                     /translation="MTLLPAPIIYTERTECQDCFKCIRECPVKAIKVENACAAVIPQL
                     CILCGHCVEICPNGAKHVRDDLQRAQTLLKEKKKVFASLAPSFISEFPDVPAHAIIRA
                     LKRLGFYGVSETALGAERVSGSIAALLQDRTPRVLISTACPVVVTFLGKYYPQYAACL
                     SPIVSPVLAHGQLLRETYGPDIGIVFFSPCIAKKGEADANPHLLDVALTFEDLRRWFM
                     SEPFSFDTAERGEDDVFVPSSAQEGALYPIDGGMVAGVKAGCSVMEAECMSFSGIRNI
                     HKALAGLGEMRLTHPLFLELLACEGGCVNGPKANQR"
     gene            complement(942..1205)
                     /locus_tag="PLM79_11510"
     CDS             complement(942..1205)
                     /locus_tag="PLM79_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOV86983.1"
                     /translation="MSGTKQPLELAICMGSSCFSRGNKRNIKLIKEYIERHGLCGKVV
                     MKGHLCEGLCKDGPNLTVDGQVFNCVDSATILALLDQHLKIQE"
     gene            complement(1202..2305)
                     /gene="hydE"
                     /locus_tag="PLM79_11515"
     CDS             complement(1202..2305)
                     /gene="hydE"
                     /locus_tag="PLM79_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOV86984.1"
                     /translation="MKPMDGDGLLGWLREEDPDKIESLWEWANTVRRRTVGDEVHLRG
                     LIEISSFCRRRCLYCGLRADNSLIERYRMSRREILESARHAQRLQFGTVVLQGGEDPG
                     CSADWVASVIRELKKESSLAVTLSLGERSYGELALWREAGADRYFLRFETSDAALYRR
                     IHPSLPSIPSDRLSILRSLRGLGYEIGSGVMIGIPGQSYRSLVEDILLFRSLDLDMIG
                     VGPFLPHPATPLGTSPRQFSRTDGEQVPATELMVTKVIALSRIFCPEANIPSTTALAT
                     LNPERGYELGLERGANVVMPNLTPHPYRSRYEIYPKKAGTGRTSFDAGEIRDRIRSMG
                     RTVGKGPGKRSPGCLRVPGSKILMGVEQFGEQP"
     gene            complement(2302..3537)
                     /gene="hydF"
                     /locus_tag="PLM79_11520"
     CDS             complement(2302..3537)
                     /gene="hydF"
                     /locus_tag="PLM79_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOV86985.1"
                     /translation="MQKTPKSMRIHIGLFGRRNVGKSSLLNAITRQQVAIVSEVAGTT
                     TDPVEKPMELLPLGPVLFIDTAGLDDEGALGDLRTRKTRQVFDRTDLGVLVTEAGTWT
                     SFEELVVQELQSRKVPGVIVVNKCDLSPGNSNSLSASLPPGIPVVRVSALTGEGLGAL
                     RRALLDQAPAELIDNPAIAGDLVGAGELAVLVVPIDKEAPKGRLILPQVQTIRDLLDS
                     DAYCMMVKDRELRKALQQLRNPPRLVITDSQAFREVAAVVPPGIPLTSFSILFARFKG
                     DLITQVRGAMAVEELRNGDQVLVAEACSHHPIGEDIGRIKIPQWLTRHVGSRLKFRHV
                     QGHDFPEDLSPYRLVIHCGACMWNRREMLGRILRCRQAGVPITNYGVVIAYSLGILER
                     ALEPFPAALETYRDLKEPR"
     gene            complement(3717..5120)
                     /gene="hydG"
                     /locus_tag="PLM79_11525"
     CDS             complement(3717..5120)
                     /gene="hydG"
                     /locus_tag="PLM79_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOV86986.1"
                     /translation="MVRSKTSSSFVNESAVAAALEGPHPGDDPVRVREILARATELKG
                     LDPEEVAALMSVRDPELMGELFSTARSVKEAVYGPRIVLFAPLYISNYCHNECLYCAF
                     RAGNRDIRRRALSQEEIAREVEILVGQGHKRLLLVAGEGYPAEGFSYILNSIATVYKT
                     RVHRGEIRRVNVNLAPCTLEEFRELKAAGIGTYQLFQETYHRETYARMHPKGRKREYE
                     WRITAFDRAMEAGIDDVGMGVLFGLYDWRFEVLALLQHIQHLERTFGVGCHTISVPRM
                     EPAAGSEIADNPPHPVSDRDFEKLVAILRLAVPYTGMIMSTRETPEIRRNTFALGVSQ
                     ISAGSRTNPGGYSDGIHEEDAQFQLGDHRSLDEVVADLSAMGYIPSFCTACYRLGRTG
                     ADFMELAKPGDIKHRCDPNALSTFLEYLLDYASPETLAAGEKLIRTHLDRMDDRHGKT
                     AAKMLDRVRKGHRDVYI"
     gene            5379..5888
                     /locus_tag="PLM79_11530"
     CDS             5379..5888
                     /locus_tag="PLM79_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86987.1"
                     /translation="MKTMQSGLIRWRTILRSAGFALLLTGIAPLFSGPAWAIQSHPAP
                     EGLYAHQLAHFLFIVAMALLSYWLQVNHFTVQRGWRLIQVSCLLFLVWNGVAILGHWS
                     EEMLPAGVLLGNSDWGQRILFQGNPAAYLYFVLKMDHLVCVPAMACLLLGIRSLYREA
                     LEERGPSHG"
     gene            5881..7431
                     /locus_tag="PLM79_11535"
     CDS             5881..7431
                     /locus_tag="PLM79_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV86988.1"
                     /translation="MVDSWSMAPVWFTDVGGSALMILLSLGAVVYAVRLVRVQPSNVL
                     WTYLLWLTAALCGFALSRGVGHIAKRLLLLMEMPDIWAVLKPYSGSFNTLAFAVVAAI
                     TFFFQRVHRIHTEILRDQEALERASAEVMRLNLGLESLVEQRTHELSRSERRYRRIFE
                     GSMDMIFVLDAEARFVDINGAGVEALGYSSPEEVVGKVSFEQVFACRAQYGELMGDLS
                     SRGYVQARECTLVARDGSERFILLSATVRKDDRGRVQSFEGIAKDVTARVHMQRQLQQ
                     ADKLASLGQISTGIAHEINNPLGIMLGYTQLLLRDMQQGSQLHDDLKTIEKHARNCKV
                     VVEDLLKFARGTRTRRTSVNVGQCLREVLSLLAHQFELDNIRIQTEIREDLPELRADG
                     EKLKQVFMNLLMNAKQAISQSGTITVRTRLDPREDALLVSISDDGCGISPENLFKIFD
                     PFFTTKPVGEGTGLGLSVSYGIIQDHQGRIDVSSRPGRGSTFTVVLPVSPPANESPGS
                     SPVERRQP"
     gene            7428..8912
                     /locus_tag="PLM79_11540"
     CDS             7428..8912
                     /locus_tag="PLM79_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV86989.1"
                     /translation="MKDPSMSLPSSATPPGPPDPDSDRDDPKPVSRLLIVDDEEDMLR
                     LLKRSLPPDLACEVETASNALRAFEVLEAAPFDVVLADIRMPGMDGMEFLERLQKEHG
                     EITVVMMTAYGTIDLAVQAIKSGAYDFITKPFEHEKLVHLLERALERSRLVHENLRLQ
                     RRLRDHESFQEIIGAGSRMQKVFEMVRLVSKTDVTVLITGESGTGKDMAARAIHRLSH
                     RSAGAFVTINCPNLPENILESELFGYRKGAFTHATQDKKGLFLEAQGGTIHLDEIGDI
                     SLTLQSKLLRVLQEKEIRPLGQTKSVRIDTRILASTNQDLPAKIRENQFREDLFYRLN
                     VLSIHMPPLRERKEDIPLLVEHFLGKYCKEFRKEPKTVEGSFLRRLMDLPWRGNVREL
                     ENFVRRGVLLAPGPVIRAEDLDWPEGSAPVCPTEGALTHLSYREAKARVLDRFHREYL
                     TAVLSRHNGNVTRAARECGLERQALQQVLKRYGISPREVGGGSG"
     gene            9229..9618
                     /locus_tag="PLM79_11545"
     CDS             9229..9618
                     /locus_tag="PLM79_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86990.1"
                     /translation="MGTDVATILVFDEEQDSRTLIQRVLERGGHEVLACERVPRAEEL
                     ASTRDPDLAVVHVGENWGGPGGIVSLLRAANRRLRVIIVADYDGRRGEECAANEILVP
                     KPLDLETLESTVRSILLGTSEKRRSDF"
     gene            9646..10818
                     /locus_tag="PLM79_11550"
     CDS             9646..10818
                     /locus_tag="PLM79_11550"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV86991.1"
                     /translation="MNKKGWMIVGLIVAVALVGTAIWTEPGMAGKLEDAISQAPVGTG
                     KGMIDPKAEPGFMGIPGAPKTYWILNILWGIWVGWIFSSVGAFGGVMAGVGHITVFGL
                     ADYGKSFKQTCPVLNKLLTDSVRVCNQYLVGASAMISSTTYYRMGRLVLPLGLCLAGG
                     GMLGAYLIPTLTAGKVDFSSYVGYFGIVVFLVAGVLFYETTPRGQASRKAAKQAAKAF
                     EDANIRKTAGTEGKTDSKSLGVHITHLGIKKITFTFYGVEFSFNPLWPVVGGFVIAAI
                     SAFIGVGGGFLYVPFLTSVAGLPMFIVAGTSALAVLVSMITSIFTFMFMKSIPVDFPL
                     ISTEMIGVFIGSVLGPVTSKYIPEVWLKRLFVVLALYVGIGYTTKGFMGRSILPGL"
     gene            10837..11439
                     /locus_tag="PLM79_11555"
     CDS             10837..11439
                     /locus_tag="PLM79_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86992.1"
                     /translation="MEMHPVYLAMDPYLIRLYRITGCSFADFLLGTFVVAFVALFVGE
                     VTASLAFLAAKKSIDRTTKDVIRYRNLSMEALAAGDKDAYEAANKLANDAFGQSFFKQ
                     IALSSAFLWPLFLALGWLNARFSGVEFTFVTAPYTVGVGCIFITLYVASYLLFRRIKY
                     RLPHFRRIKTMLDAYDARSVEVKKLSDVFPLPDSAVQRTS"
     gene            11461..13584
                     /locus_tag="PLM79_11560"
     CDS             11461..13584
                     /locus_tag="PLM79_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV86993.1"
                     /translation="MSESARKKVYSVCAVCSVRCPVEVDVEGGAVKHIWGNPHLMGGR
                     YLCPRGTAQKVFQTDTERVQYPMIRDGERGSGKWRKATWEEALDYVAQKLRGIIDQYG
                     GESVVLADRGGPFTDLHKAFIKALGSPNYFNQHACCSNSVHNAHNAMAGQRRNTVTYD
                     WKNCKYTVLYARNILESIGTKEAKDFMDAREKGMKVVYLDARWTYTAAKSDRFFILRP
                     GTDYAFNLALIHVLVKEKLYDEEFVRNWVLGMKELTAFIEPYTPEWAEKETGVSADEI
                     RAVARELTQAAPSVILHQGWMTARHEDDYYFRRSIYILYGLLGAYEAPGGLLFNKNET
                     HCGYKPLRKFVDLPPRVDKKRFDGIGWKYKHLSADYGLGQMLPYAILNEDPYPIKAFL
                     CYRFDPLSSYPDPEAFKEGLMKLDLLVSVDVNYSHIAWVSDVILPEAMFLERTDPVIV
                     KGGPKPALWIRRQAVEPRYDSKPKWWIIKQLAERLGVGQYFPYNTMEEMIAWQLQDMG
                     FKLSDLEEKGCIELSKEPILFDRKTGLTFKTPSKKLEFVSSMLEENGIPSFPPYRSPA
                     PPPEGHYRLITGKIAVHTQGTTLNNLYLNEIQSENKLWINSEEAKKLGIKAGDAVEVS
                     GGGIAQTGIAAVSEYVHPEVVYTLHGYGREVPLQTRAYKKGMRDNTLMKGLLKVAVGG
                     NCPVADCFIRIRKASRGDEGQERTL"
     gene            13581..14093
                     /locus_tag="PLM79_11565"
     CDS             13581..14093
                     /locus_tag="PLM79_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV86994.1"
                     /translation="MSSYYIYQDQERCIGCRACELHCKTENDVPVGPSFCKIIQVGPI
                     LRGHIPRVHFVFMPCFHCEAPWCVSVCPTGAMQKRDRDGIVFVDHSLCIGCKACITAC
                     PWGAPQWNPRTGTVIKCDYCKDRIDEGLEPACVTGCTTAALKWVSPAESTRMRREFFA
                     RALAGSRSIP"
     gene            14121..>16052
                     /locus_tag="PLM79_11570"
     CDS             14121..>16052
                     /locus_tag="PLM79_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV86995.1"
                     /translation="MSSPPFFSPGPGADKDRIVYDTDGLKRESSAPCQLHCPAGIDVP
                     SYVALVGHGRYEEAVEVIRRDNPLPWVCGLVCPNPCESWCQRRHLDKPIAIKDVKGFA
                     ARMVWEKGLDYRNPEPKNRFEEKVAVVGSGPAGLSAAYFLAWEGYPVTVFEAMQEPGG
                     LLIAGIPEYRLPRHVVRREIEALRKMGIEFVVNTPVGKDLTLDHLRKDGYKAFFLGVG
                     AWDSVRIGIEGEDEYPQVIPVLDFLKDVSFGKKIKPANSVAVIGGGNAAIDAARTLVR
                     LGAQSVSIVYRRTRSEMPAHFEEIIQMAEEGIHVHQLTVPVKIGGSYGKVDYLECVMA
                     TLGEPDESGRRRPVPIPDSNYRMPVGAVVSAIGQRTDVLAYPGLGALDLSRYGNIQVR
                     ERGRQTNIPDVFAGGDAVTGPATVIQAIAGGKKAARGIHAYLRGMDPPWEAVPRPRSM
                     VEPILMDYRQKAFIQRQEIPLIDLDRRIHTFDQVELGLDEIAARQEARRCMRCDVCER
                     CGKCAEVCHERLGVSAIAFHHSGESSLILKDYVHGLPRCIGCGACVNICPTGALQMED
                     RGDTRILSMCGTVINKIRLDRCEGCGAYHVPTVLVHHVSNLVGVPHELVDRKLCPGCK
                     RIVHAAAVAGSPPVFPQPAA"
BASE COUNT         3098 a         4925 c         4904 g         3125 t
ORIGIN      
        1 cgctgattgg ccttgggacc gttgacgcac cctccttcgc aggcaagcag ttccaggaag
       61 agggggtggg tgaggcgcat ctcgccgagc cccgccaggg ctttgtgaat gttgcgaatg
      121 ccggaaaagg acatgcattc cgcttccatg acgctgcacc cggccttcac tcccgccacc
      181 atgccgccgt cgatggggta cagggcaccc tcctgggccg aggagggaac aaagacgtca
      241 tcctctcctc tctctgccgt gtcgaaggaa aaaggctcgc tcatgaacca tcgtcggagg
      301 tcctcgaagg tgagggcgac atccagcagg tgggggttcg cgtccgcctc tccctttttg
      361 gcgatgcatg ggctgaagaa gacgatcccg atatccgggc cgtaggtctc ccgcagcaat
      421 tggccgtggg ccaggaccgg ggagacgatg ggagacaggc aggcggcata ctggggataa
      481 tacttgccca agaaggtgac caccaccgga caggccgtgg atatgagcac tcgaggcgta
      541 cgatcttgaa gcaaagcggc aatgctgccc gaaacccttt cggcccccag ggcggtttcg
      601 gagacgccgt aaaaccccag acgcttcagg gcccgaatga tcgcgtgggc cggaacgtcc
      661 gggaactcac tgatgaagga cggagccagg gaggcaaaga cctttttctt ctctttgaga
      721 agggtctggg cccgttgcag gtcgtcgcgc acatgcttgg ctccgttggg acagatctcc
      781 acacagtggc cgcacagaat gcataactgg gggatgaccg ccgcgcaggc gttttcgacc
      841 ttgatggcct tgacggggca ctcgcgaatg cacttgaagc agtcctggca ttcggtcctc
      901 tccgtataga tgatgggagc gggcagaagg gtcattcctc tctactcctg gatcttcagg
      961 tgttggtcga gcaacgcaag gatcgtcgcg gaatccacgc agttgaagac ctggccgtca
     1021 acggtcagat tgggtccgtc cttgcacaac ccttcgcata gatgtccttt catgaccacc
     1081 ttgccgcaaa gaccatggcg ctcgatgtat tccttgatga gtttgatgtt gcgcttgttg
     1141 cctctggaaa agcatgaact gcccatacag atggccagtt ccaggggttg tttcgtgccg
     1201 ctcatggttg ttctccaaat tgttcaacac ccataaggat cttgctcccg gggaccctca
     1261 ggcatcctgg agaccgcttc cccgggcctt tgccgacggt acgtcccatg gaccgtatgc
     1321 ggtcccggat ctcacctgca tcgaacgagg tccgaccggt ccctgccttc ttggggtaga
     1381 tttcgtacct gctgcggtat ggatgggggg tgaggttcgg catgacgacg ttggcgcccc
     1441 gctcgaggcc cagttcgtac ccgcgctcgg gattcagggt cgccagggcg gtcgtgctgg
     1501 gaatgttcgc ctcggggcaa aaaatcctgg acaatgcaat taccttggtc accatgagct
     1561 ccgtggcggg gacctgttcc ccgtcggtcc tcgagaattg ccggggagag gtgcccagag
     1621 gcgttgccgg gtgaggcaga aacggaccca ccccgatcat atccagatcc aacgaccgga
     1681 acaacaggat atcctccacc aggctgcggt aactctgacc ggggattccg atcatgacgc
     1741 cgctgccgat ttcatacccc agccctcgga gggaccggag aatggatagg cggtccgagg
     1801 ggatcgaggg aagggaaggg tggattcgtc ggtacagggc ggcatcggac gtctcgaatc
     1861 gcaggaagta acggtcggct ccggcttccc tccacaacgc cagttcgccg taggaccgtt
     1921 ctcccaggct caaggtcacg gccagggagc tttccttctt gagttcccgg attaccgagg
     1981 ccacccagtc tgcggaacat cccgggtctt cgcctccttg caacaccacc gtgccgaatt
     2041 gcagcctctg cgcatggcgg gcgctctcga ggatctcccg gcggctcatc cggtagcgct
     2101 cgatgaggga gttgtccgcg cgcaacccgc agtagaggca acgccgcctg cagaagctcg
     2161 agatctcgat caatccccgc aggtggacct cgtctcccac ggtccgcctg cggacggtgt
     2221 tcgcccattc ccagagggat tcgatcttgt ccgggtcttc ctcccggagc catccgagca
     2281 gtccgtcgcc gtccatgggc ttcatcgcgg ttccttgagg tcccgatagg tctccagtgc
     2341 ggccggaaaa ggctccagcg cccgctccag gatccccaga ctgtaggcga tcacgacccc
     2401 gtaattggtg atgggaaccc cggcctgtcg gcaacgaagg atccggccga gcatctccct
     2461 gcgattccac atgcaggccc cgcaatgaat caccagccgg tagggagaaa gatcttcggg
     2521 gaagtcgtgt ccctgaacgt gccggaactt cagccgggag cccacatgtc gggtgagcca
     2581 ctgggggatt ttgatccgcc cgatgtcctc cccgatggga tgatgactgc aggcctcggc
     2641 aacgaggacc tggtccccgt tcctgagttc ttccacagcc atggctcccc ggacctgggt
     2701 gatgaggtcc cccttgaatc gagcgaaaag aatcgagaag ctggtcaaag ggatccccgg
     2761 aggcaccacg gcggccacct ctcggaaagc ctgggagtcg gtgatcacca gtcgcggcgg
     2821 gttcctcagc tgttgaaggg cctttcgcag ttcccgatcc ttcaccatca tgcagtaggc
     2881 atcgctgtcc agcaggtctc gaatggtctg gacctggggc aaaatgagcc gtcccttggg
     2941 ggcttccttg tcaatgggaa ccaccaggac cgcgagttct cccgctccca ccaggtcgcc
     3001 cgcaatggcg gggttgtcga tgagctcggc cggggcctgg tccaagaggg cccggcgcag
     3061 cgcccccagc ccctctccgg tcaaagcgct cacccgcacc acgggaattc cgggcggcaa
     3121 ggaggctgaa agggagttcg agttccccgg actgaggtcg cacttgttga cgacgattac
     3181 cccggggacc ttccgggatt gcaattcttg caccacgagc tcttcgaagc ttgtccaggt
     3241 gcccgcttcg gtcacgagga ctcccaggtc ggtccggtcg aagacctggc gcgtcttgcg
     3301 ggtgcgcagg tcccccaggg ccccctcgtc gtccaggccc gccgtgtcga tgaagagcac
     3361 cggccccagg gggagcagtt ccatgggctt ttccaccggg tcggtggtgg tgcccgccac
     3421 ctccgatacg atggcgacct gctggcgtgt gatcgcattg agcaggctcg atttgcccac
     3481 gttgcgccgc ccgaacaacc cgatgtggat gcgcatgctc ttgggtgtct tctgcatgaa
     3541 atccccgtcc tgcggttctt gttcgccgga tgccctcggt atttggccgt cggcaaggag
     3601 gaatgggttc aaggtcccat ccgggttttg cggtggggcc cccgaaacgg cacacgccct
     3661 cgggggcgga tgaacccttc gaaccgtgtc gagagaatcc ggcgggatcc gcgccgtcaa
     3721 atgtagacgt cccgatggcc cttgcgaacc cgatccagca tttttgcggc cgttttcccg
     3781 tgccggtcgt ccatgcggtc cagatgggtc cggatgagct tctcgcctgc ggccagggtt
     3841 tccggggagg catagtccag caggtactcg agaaacgtcg acagggcgtt gggatcgcac
     3901 cggtgcttga tgtccccggg cttggccagc tccatgaaat ccgcccccgt tcgccccagg
     3961 cgataacagg cggtgcagaa ggaaggaatg tagcccatgg cggacaggtc ggcaacgacc
     4021 tcgtccaagg aacgatggtc ccccaactgg aactgggcgt cctcctcatg aatcccgtcg
     4081 gaatatcccc cggggttggt gcggctcccg gcggagattt gggagactcc cagggcgaag
     4141 gtgtttctcc ggatttccgg cgtctcccgc gtggacatga tcatgcccgt gtagggcact
     4201 gccaggcgga gaatggccac gagcttttcg aaatcccggt cgctcacggg atgcggggga
     4261 ttgtcggcga tttcggaacc cgcggcgggc tccatgcggg gaacactgat ggtatggcac
     4321 cccaccccga acgtgcgctc caggtgttga atgtgctgca ggagggccag gacctcgaag
     4381 cgccagtcgt aaagcccgaa gagcaccccc attcccacgt cgtcgattcc ggcttccatg
     4441 gcgcggtcaa aggccgtgat gcgccattcg tactcccgct tgcgcccctt cgggtgcatc
     4501 cgtgcgtagg tttcccggtg ataggtttcc tggaacagct gataggtccc gattcccgcg
     4561 gccttgagct cccggaactc ttcgagggtg cagggggcca ggttcacgtt gaccctgcgg
     4621 atctctcccc ggtgaacccg ggtcttgtag accgtggcaa tggagttgag aatgtaggaa
     4681 aacccctcgg caggatagcc ttcccccgcc acgagcagga gtcgcttgtg gccctgcccc
     4741 accaggattt ccacttcccg ggcgatttct tcctgggaga gggcccggcg gcggatgtcc
     4801 cgattgccgg cccggaaggc gcaatagagg cattcgttat gacagtagtt ggaaatgtaa
     4861 aggggagcaa acaaaacgat gcgggggccg tagacggctt ccttgacgga tcgtgccgtg
     4921 gagaaaagct cccccattag ctcggggtcc cggacggaca tcagggcggc cacttcctcg
     4981 gggtcgagcc ctttcagttc cgttgcccgg gcaagaatct cgcgaacccg cacgggatcg
     5041 tccccgggat gcggcccctc gagagcggcc gcaacggcag attcattcac gaaagagctg
     5101 cttgtcttcg atcgaaccat aaccttcacc cgagcggtca cacgtcaatt gtgacagcga
     5161 tcgcaagcaa gaactgaaga accaccggga cggggaaccg agtcaatcgc tgtgatttta
     5221 ttccctttga caattctttc aagacgttcc tgcggaaaaa gcggtgtcgc attcgctccc
     5281 catgccccat gaaattctcg aaaaggtatg gtaggtatag gggtgcgcac gcgggccttg
     5341 tcggtgccgt ccaacaaccc cgggggacga ttgcttccat gaagacgatg caaagtgggc
     5401 tcatccgttg gcggacgatc ttgaggtcgg caggattcgc ccttctgctc accgggatcg
     5461 ctcccctctt ttccggtccc gcctgggcca tccagtccca tcccgccccc gaggggcttt
     5521 atgcccatca gctggctcat tttctcttca tcgtggccat ggcccttctt tcctactggc
     5581 tgcaggtgaa tcacttcacg gtccaaagag gttggcgcct cattcaggtc tcctgcctcc
     5641 tgtttcttgt ctggaacggg gtggcgatcc tgggccattg gtcggaggaa atgcttcctg
     5701 ccggggttct gttggggaat tccgactggg gccaacggat cctcttccag ggaaaccccg
     5761 cggcctacct ctactttgtc ctcaagatgg accacctggt ttgtgtgccc gccatggctt
     5821 gcctgctcct gggaattcgc agcctctacc gggaggccct cgaagagcgg ggaccctccc
     5881 atggttgatt cctggtccat ggcccccgtc tggtttaccg acgtgggggg gtcggccctc
     5941 atgatccttt tgtccctggg agccgtggtg tatgccgttc gcctggtcag ggtgcagcct
     6001 tccaatgtcc tttggaccta tctcctctgg ctcaccgcgg ccctctgcgg ttttgccctg
     6061 tcccggggag tggggcacat cgccaagaga ctcctattgc tcatggagat gccggatatc
     6121 tgggcggtat tgaagcccta cagcggctcc ttcaataccc tcgccttcgc ggtggtggcc
     6181 gccattacgt tctttttcca gcgggtgcac cggatccaca cggagatcct tcgggaccag
     6241 gaggccctgg aacgggcttc agccgaagtg atgcgcctca acctcggcct ggaatccctg
     6301 gtcgagcagc ggactcacga actgagccga tccgagagga gatatcgccg catttttgaa
     6361 ggttccatgg acatgatttt cgtcctggac gcagaggccc gcttcgtgga catcaacggc
     6421 gcaggggtcg aagccctggg gtattcgtct cccgaagaag tggtgggaaa ggtttccttc
     6481 gaacaggtct ttgcctgccg tgcccagtac ggggaactca tgggcgatct ttcgtcccgg
     6541 gggtacgtcc aggcccggga gtgcaccctg gttgcccggg acggctccga acggttcatc
     6601 cttctcagcg ccaccgtgcg gaaggacgac cggggccgtg tccagagctt cgagggcatc
     6661 gccaaggacg tgaccgcccg ggtccacatg cagcggcagc tccagcaggc agacaagctg
     6721 gcttccctgg gacagatttc cacgggaatc gcccatgaaa tcaacaaccc cctgggaatc
     6781 atgttgggct acacccagct gctccttcga gacatgcagc aggggagcca gctccacgac
     6841 gatctcaaga ccatcgaaaa acacgcccgc aactgcaagg ttgtggtgga agatctgctc
     6901 aagttcgccc ggggcacccg aacccgccgc acatcggtca atgtcggcca gtgcctgcgc
     6961 gaggtgttgt cgctgctggc acaccagttc gaactggaca acatccggat tcaaacggag
     7021 atccgggagg atttgccgga gctccgagcc gatggggaga agctcaaaca ggtcttcatg
     7081 aacctcctta tgaacgccaa gcaggccatt tcccaatcgg gcaccatcac cgtccggacc
     7141 cggctggacc cgcgggagga cgccctcctg gtttccattt ccgatgacgg ttgcggcatt
     7201 tctcccgaga acctgttcaa gatcttcgac ccgttcttca ccaccaaacc cgtgggggaa
     7261 gggaccggcc tgggactttc ggtgagctac ggaatcattc aggaccacca ggggcgtatc
     7321 gacgtctcga gtcgccccgg caggggaagc accttcaccg tggtcctccc ggtgagcccc
     7381 cccgcgaacg aatcccccgg ctcctctccc gtggaaagaa gacaaccatg aaggacccct
     7441 ccatgtccct gccttcttcg gcaacgccgc ccgggccccc ggaccccgat tccgaccggg
     7501 acgacccgaa gcccgtatcc aggctcctca tcgtcgacga cgaggaagac atgctccggc
     7561 tgctcaagcg cagtcttccg ccggacctgg cctgcgaggt ggaaacggcc tccaatgccc
     7621 tccgtgcctt cgaagtgctc gaggccgcac ccttcgatgt cgtcctggcc gacatacgca
     7681 tgcccggcat ggacggcatg gaattcctgg agcgccttca aaaagagcac ggcgaaatca
     7741 ccgtcgtgat gatgaccgcc tacggaacca tcgatcttgc cgttcaggcc atcaagagcg
     7801 gggcctatga tttcatcacc aagcctttcg aacatgaaaa gctggttcac ctgctggaaa
     7861 gggccttgga acgaagccgg ctggttcacg aaaacctgcg cctccaaaga cggcttcggg
     7921 atcacgagag cttccaggag atcatcggtg ccgggtcccg gatgcagaag gtcttcgaaa
     7981 tggttcgcct ggtcagcaaa acggacgtca cggtgctcat cacgggggag tcgggtaccg
     8041 gaaaagacat ggcggcccga gcgattcacc ggctgagcca tcggagcgcc ggggcgtttg
     8101 tgaccatcaa ctgccccaac cttcccgaga atatcctcga gagcgaactc ttcggatacc
     8161 ggaaaggcgc cttcactcat gccacccagg acaagaaggg tctttttctc gaggcccagg
     8221 gaggcacgat tcacctggac gaaatcgggg acatttccct caccctccag agcaagctcc
     8281 tgcgagtcct ccaggaaaaa gaaattcggc ccctggggca gaccaagagc gttcgcatcg
     8341 acacccgcat ccttgcctcc accaaccagg acctgcccgc caaaatccgg gagaaccaat
     8401 ttcgcgaaga cctgttctac cggctcaacg tcctgtccat ccacatgccg cccctcaggg
     8461 agcgcaagga ggacatccct ctcctggtgg aacacttttt gggaaaatac tgcaaggaat
     8521 tcagaaaaga gcccaagacc gtggagggct ccttcctgcg ccgcctcatg gatctgccct
     8581 ggaggggaaa cgttcgggaa ttggaaaatt tcgtgcggag gggggtcctc ctggcgccgg
     8641 gtccggtcat acgggccgaa gacctggact ggcccgaagg ctcggccccc gtctgcccca
     8701 cggaaggggc cctaacccac ctttcctatc gagaggccaa ggcccgggtc ctggaccggt
     8761 tccaccggga atacctgacg gccgtcctgt cccgtcataa cggcaacgtc acccgggcgg
     8821 cccgggaatg cgggctcgag agacaggccc tccagcaggt gctgaagcgc tacgggatca
     8881 gccccagaga ggtgggaggg gggagtggtt agtgggttgg ttgatcggtt ggggtgaagg
     8941 tgcagcaaaa ggattgcagt gcaaggtttt ttctccctta aagacaatgt attgaatccg
     9001 cgatagaaaa tccgtttggt ccctccccgg gcgcaaggat tctcttgcgc cgggggatcc
     9061 cgtcgccaaa tcccgccgtg tttctcaaat cctgtttatc tcaccattgc aatggcttat
     9121 ggacaaagac ccatccaggg gccgcggaag aatccgccgg cacgatcctt gttccatgcc
     9181 cggtggccgg gcgaggcccg acaaggacgg attctcagga actcaatcat gggaacggat
     9241 gtggcaacca tcctcgtatt cgatgaggag caagattccc ggacgttgat tcaaagggtc
     9301 ctggagcggg gcgggcatga ggtcctcgct tgcgagcggg taccccgggc cgaggaactg
     9361 gcctcgacga gggacccgga cctggccgtc gtccacgtcg gcgagaattg gggcggaccg
     9421 ggagggatcg tttctctcct cagggccgcc aatcgtcgtc tccgggtcat catcgtcgcc
     9481 gactacgacg ggcgaagggg ggaggaatgc gcggccaatg agattcttgt tcccaagccg
     9541 ttggacctcg aaaccctgga atccacggtt cgctctattt tattgggaac ctcggagaaa
     9601 cgcagatcgg atttctaaat aaaccaaatc cccaaggaga caggcatgaa caagaaaggc
     9661 tggatgattg tgggtctaat cgttgcggtg gcgttggtgg gcaccgcgat atggacggag
     9721 ccgggcatgg cgggcaagct cgaggacgcc atttcccagg cgccggtggg gacgggaaag
     9781 ggcatgatcg atcccaaggc cgagcccggg ttcatgggaa ttcccggggc tcccaagacc
     9841 tattggatcc tgaacatttt gtgggggatc tgggtcggct ggatcttttc ctcggtgggg
     9901 gccttcggag gcgtcatggc gggggtcggc cacatcacgg tcttcggcct ggcggactac
     9961 ggcaagagct tcaagcagac ctgcccggtc ctcaacaaac ttctcaccga ttcggtgcgg
    10021 gtgtgcaacc aatacctggt aggggcttcg gccatgattt cttcgaccac ctattaccgc
    10081 atgggacgac tggtgctccc cttgggcctg tgcctggcgg gaggcggaat gctgggcgcc
    10141 tacctcatcc cgaccctcac cgccggaaaa gtcgatttca gctcctacgt tggatacttc
    10201 ggaatcgtgg tcttcctggt ggcgggcgtc cttttctacg aaacgactcc cagggggcag
    10261 gcgagccgga aagcggccaa acaggcggcc aaggccttcg aggatgccaa tattcgcaaa
    10321 accgccggaa ccgaaggaaa gaccgactca aagtccttgg gagtacacat tacccatctg
    10381 gggatcaaga agatcacctt cacgttctac ggggtggaat tctcgttcaa ccccctctgg
    10441 cccgtagtgg gaggcttcgt catcgcggcc atttccgcct tcatcggggt cggcggcggc
    10501 ttcctctacg tcccgttcct gacctcggtg gcgggtcttc ccatgttcat cgtagcgggg
    10561 acttcggccc tggccgtcct ggtgagcatg atcacctcga ttttcacctt catgttcatg
    10621 aagagcattc ccgtggactt tcccctcatc agcacggaga tgatcggggt cttcatcggt
    10681 tccgtgttgg ggccggtcac ctccaagtac atccccgaag tgtggctcaa acggctgttc
    10741 gtcgtcctgg cgctctacgt aggaatcggt tacaccacca agggattcat gggccggtcc
    10801 attttgcccg gactctagga acattggagc aactccatgg aaatgcaccc ggtttacctg
    10861 gccatggacc cgtacctgat ccggctctac cggatcacgg gttgcagctt tgcggacttt
    10921 ctcctgggaa ccttcgtggt ggccttcgtc gcccttttcg tgggagaggt caccgcttcc
    10981 ctggccttcc tggccgccaa gaaatccatt gacaggacca cgaaagatgt gatccgctac
    11041 cggaatctgt ccatggaagc cctggcggcg ggagacaagg atgcctacga ggcggccaac
    11101 aaactggcca acgatgcctt cggacaatcc tttttcaagc aaatcgccct gtcctcggct
    11161 ttcctgtggc cgctgtttct ggccctggga tggctgaatg cccggttttc cggggtcgaa
    11221 ttcaccttcg tcacggcgcc gtacaccgtg ggagtcgggt gcattttcat caccctctat
    11281 gtggcatcct atctcctgtt tcgccgaatc aagtatcggt tgccccactt tcgaaggatc
    11341 aagaccatgc tggatgccta cgatgcccgg tcggtcgagg tgaagaagct gtcggatgtt
    11401 tttcctctcc ccgattcggc cgttcagcgg acttcgtaaa gtagaacaga ggaagagaac
    11461 atgtcggaat ctgccaggaa gaaggtttac agcgtttgtg ccgtgtgcag cgttcggtgc
    11521 cccgtcgagg tggatgtgga aggcggcgcc gtcaagcaca tttgggggaa tccccacctc
    11581 atgggcggcc ggtatctgtg tccccgggga acggcgcaaa aggtgttcca gacggacacc
    11641 gagcgcgtcc agtaccccat gattcgggat ggggagcggg ggtccggaaa gtggaggaag
    11701 gctacctggg aggaagccct ggattacgtc gcccaaaaac tcaggggcat catcgatcag
    11761 tacggcggag agagcgtggt ccttgcggac cggggaggtc ccttcaccga tctgcacaag
    11821 gccttcatca aggccctggg gtcgcccaac tacttcaacc agcacgcctg ctgttccaac
    11881 agcgtccaca acgctcacaa cgccatggcg ggacaaaggc gcaataccgt cacctatgac
    11941 tggaagaact gtaagtacac cgtcctgtat gcccgcaaca tcctcgaatc catcggcacc
    12001 aaggaagcca aggatttcat ggacgcccgg gaaaagggaa tgaaagtggt ctacctggac
    12061 gcccggtgga cgtacacggc ggccaagtcc gaccggtttt tcattcttcg tcccgggacc
    12121 gattacgcct tcaacctggc cctcatccac gtcctcgtca aggaaaagct ctacgacgag
    12181 gaattcgtcc ggaactgggt gctggggatg aaggagctca cggccttcat cgagccctac
    12241 actcccgaat gggccgagaa ggaaacgggt gtctcggcgg acgaaattcg cgccgtggcg
    12301 cgtgaactga cccaggccgc cccctcggtc attctccacc agggctggat gacggcccgg
    12361 cacgaggacg actactactt tcggcgctcc atctacatcc tgtacggtct cctgggggct
    12421 tatgaagccc cgggagggct cctcttcaac aagaacgaga cccattgcgg ctacaagccc
    12481 ctgcgcaagt tcgtggatct tccccccagg gtggacaaga agcgcttcga cggaatcggc
    12541 tggaaataca agcacctgtc cgccgattac gggttggggc agatgctccc ctacgccatc
    12601 ctcaacgagg atccttaccc catcaaggcc ttcctctgct accggttcga tcccctgtcc
    12661 tcgtacccgg atcccgaggc cttcaaggaa ggcctcatga agctggattt gctggtttcg
    12721 gtggacgtca attacagcca cattgcctgg gtgtcggacg tgatccttcc cgaggccatg
    12781 ttcctggaaa gaacggaccc cgtcatcgtc aagggaggac ccaaaccggc cctctggatt
    12841 cggcgccagg ccgtcgagcc ccgctacgac agcaaaccca aatggtggat catcaagcag
    12901 ctggccgaac gactgggagt cggccaatat tttccctaca acacgatgga agagatgatc
    12961 gcctggcagc tccaggacat ggggttcaag ctctcggacc tggaggaaaa gggttgcatc
    13021 gagttgtcca aagaaccgat ccttttcgac cgcaagaccg gtttgacctt caaaacgcct
    13081 tcgaaaaagc tcgagttcgt ctcgagcatg ctcgaggaaa acgggatccc gtcctttcct
    13141 ccctaccgga gccccgcgcc gccgcccgaa ggccactatc gtctcatcac cggcaagatc
    13201 gccgtccaca cccagggaac gaccctcaac aacctctacc tcaacgaaat ccagtcggag
    13261 aacaagctct ggatcaacag cgaagaggcc aagaagctgg gaatcaaggc cggggacgcg
    13321 gtggaagtct ccgggggcgg aattgcccag acgggaattg cggcggtcag cgaatacgtc
    13381 cacccggaag tggtttacac cctccacggg tacggccggg aagtgcctct ccaaacgagg
    13441 gcctacaaga aggggatgag ggataacacc ctgatgaagg ggctgctgaa ggtggccgtg
    13501 ggaggcaact gcccggttgc cgattgtttt atccgcatca ggaaagcgtc tcggggcgac
    13561 gaaggacagg agcgaaccct atgagcagct actatattta ccaggaccag gaaaggtgca
    13621 tcggatgccg ggcatgtgag ctgcactgca agaccgaaaa cgacgtaccc gtggggccgt
    13681 ccttctgcaa gatcatccag gtgggcccca tcctcagggg tcacattccc cgggtccact
    13741 tcgtcttcat gccctgcttt cattgcgagg ccccctggtg tgtttccgtc tgtcctacgg
    13801 gggccatgca gaaacgggac cgggacggga tcgtctttgt ggaccattcc ctgtgcatcg
    13861 ggtgcaaagc gtgcatcacc gcatgtccct ggggggctcc ccagtggaat ccccggacgg
    13921 ggaccgtcat caagtgcgac tactgcaagg accgaatcga cgaggggctc gagcccgcct
    13981 gcgtgacggg ctgtaccacg gcggccctca aatgggtctc ccctgcggaa tccacccgca
    14041 tgaggcggga gtttttcgct cgagccctgg ccgggtcccg gtccattccc tgatacccct
    14101 ggatatttcg ggagccgatc atgtcttctc ctccgttctt ttcccccggg cccggcgcgg
    14161 acaaggaccg catcgtttac gacaccgacg gtctgaagag ggaatcgtcc gccccctgtc
    14221 agcttcactg tcctgccggg atcgacgttc cctcctacgt tgccctggtc ggccacggcc
    14281 ggtacgagga ggccgtggag gtcatccggc gggacaatcc ccttccgtgg gtgtgcggcc
    14341 tggtttgtcc caacccgtgc gagtcctggt gtcaacgacg gcacctggac aaacccattg
    14401 ccatcaagga cgtgaagggg tttgcggcgc gaatggtttg ggaaaaaggc ctcgattacc
    14461 gaaaccccga gcccaagaac cgtttcgagg aaaaggtggc cgtcgtcggg tccggtccgg
    14521 ctgggctgtc ggcggcctat ttcctggcct gggagggtta cccggtcacg gttttcgagg
    14581 ccatgcagga acccggcggc ctgctcatag ccggaattcc cgaataccgg cttcctcgtc
    14641 acgtggttcg ccgggagatc gaagccctcc ggaagatggg gatcgagttc gtggtgaaca
    14701 cccccgtggg caaggacctc accctcgatc acttgcgtaa ggacggctac aaggccttct
    14761 tcctgggcgt gggagcctgg gacagcgttc gcatcggcat cgagggagag gacgaatatc
    14821 cccaagtgat tcccgttctc gatttcctca aggacgtgtc tttcgggaag aagatcaaac
    14881 cggccaacag cgtggccgtc atcgggggag gaaacgccgc catcgacgcc gcccgcaccc
    14941 tggtgcggct gggcgcccag tccgtgagca tcgtgtatcg gaggacccgc tcggagatgc
    15001 ccgcccactt cgaggaaatc atccagatgg ccgaagaagg gatccatgtc catcagctca
    15061 cggtgccggt caagatcggg ggaagctacg gcaaggtgga ttacctcgag tgcgtcatgg
    15121 ccaccctggg ggaaccggac gagtcggggc gccgtcggcc cgtacccatc cccgactcca
    15181 actaccggat gcccgtgggc gcggtggtgt cggccatcgg ccagaggacc gacgtgcttg
    15241 cctacccggg actgggggcg ctggacctgt cgcggtacgg aaacatccag gttcgggagc
    15301 gcgggcggca gaccaacatt ccggacgtct ttgccggggg ggatgcggta acggggcccg
    15361 ccaccgtcat ccaggccatc gccgggggca aaaaggcagc ccggggaatt catgcctacc
    15421 tgaggggaat ggaccccccg tgggaagcgg ttccgcggcc ccgatccatg gtcgagccca
    15481 tccttatgga ctaccggcag aaggctttca tccagaggca agagatccct ctcatcgacc
    15541 tggaccggag gattcatacc ttcgaccagg tggaattggg actggacgag attgcggctc
    15601 ggcaggaagc ccggcggtgc atgcggtgcg acgtgtgcga gcggtgcggc aagtgcgccg
    15661 aggtctgtca cgaaaggttg ggggtctcgg ccatcgcctt tcaccattcg ggggagagct
    15721 ccctgatcct caaggactac gttcacgggc tgccgcgctg catcggttgc ggagcctgcg
    15781 tgaacatctg ccccacgggg gcccttcaaa tggaagaccg gggagacacg cgcatcctgt
    15841 ccatgtgcgg gaccgtcatc aacaagatcc ggctggaccg gtgcgagggt tgcggagcct
    15901 accatgtccc cacggtcctg gttcaccacg tgtcgaacct cgtgggtgtt ccccatgaac
    15961 tcgtggaccg gaagctgtgc cccggatgca aacggatcgt tcatgccgcc gccgtcgccg
    16021 ggtccccacc ggtcttcccc cagcccgccg cc
//
LOCUS       DAOOVC010000088        16029 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2449_length_16029_cov_6.176975, whole genome shotgun sequence.
ACCESSION   DAOOVC010000088 DAOOVC010000000
VERSION     DAOOVC010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16029
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_16029_cov_6.176975"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..84)
                     /locus_tag="PLM79_11575"
     tRNA            complement(10..84)
                     /locus_tag="PLM79_11575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Thr,seq:cgt)
     gene            413..697
                     /locus_tag="PLM79_11580"
     CDS             413..697
                     /locus_tag="PLM79_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV86996.1"
                     /translation="MSKCIGSLNISLMCDEHDTAGEFFTLSASKYIQKFIGTSLPPDM
                     RALISSGQPSRWRIGDMDVIIDFICAQSARSKQSSREMKLVITNGDKALG"
     gene            1789..4848
                     /locus_tag="PLM79_11585"
     CDS             1789..4848
                     /locus_tag="PLM79_11585"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOV86997.1"
                     /translation="MTRRMKTIIAEALVFVLVLSSASMAQNLQAVPINPYPQQTIPSP
                     PTMPGGYAQSMGTPQAQAQMSPLGPAPIAQPQPQVYHPGQIAPAPGVVGATSYPAGMQ
                     TPQTFPVQPGVPATQDISQAVPSSAFATTEEEPPSPFEQYVSGVIPSTLSFDIKQFGY
                     DVFYKPSPTTFVPLKPVPGPSIQQPRGTTQPGGGFPGVPTQGVPGSVVSPQAPGGTLQ
                     GWPGMIPSGVPGQQVPQLSPAQPGLQPGGASSALSQKAAQLQGLLGPGANVQALVPQA
                     QSPQARGQVPGTATSAAAALQSQIPSGTSVPYLRNNQYPLLQPVADVPVGPDYVLGPG
                     DEVRVAVWGSVEGTWFVSVDRDGNITLPKVGVVGVAGLTYQELKEVLQKQLSKYYTDF
                     EMNVSLGALRSIRVYLVGNARTPGAITVSSLSTIINALMVSGGPTKTGTLRNIELKRK
                     GETITHLDVYDFLIRGDKTNDTRLMPEDVILIPPIGPIVGIAGNVATPAIYELKGKTR
                     LSEGIKMAGGVTAAAYLQRVQVERVSKKQNKIILDLNLDKVKGKDDIYLEDGDIVKVF
                     PINTVVTNRVVLQGSVRRAGEYEWKPGMRVKDLIPNTEALLPNTLFTYAAIERVVPPD
                     YHPEYVTFSLEGLLIRKEEKENVKLEPFDVVTVYAKPDKLEKQVVQITGAVNTPGQFE
                     FRPNMKLSDLVNIAGGLRPYAFMNSAEVTRVIPTQQGPKTEQIYVNLSEALAGNPQQD
                     ITLMENDYLFVRSVPDWQIYKKVYVYGEVNFPGEYAFKKGETVSSVIERAGGYTSRAY
                     LRGTVFTRVSTKQVQQAQINEMVDRLEREMAAAGTADLTVATDPVEARVIQYQNAQKR
                     VFVEGLRRVEAKGRMVVRLDHPAKMKKTSFDIPLEEGDMIFIPTNPQTVQVLGAVYNQ
                     TSFVYEPKRDYMYYVTKTGGYTSSADKKAVYVLKCDGTSVKPGSGLTWDHDSNEWRTG
                     GSVVLEPGDTIIVPDQLDKMGWYVALKGITEVIYRIAVGTGILFRAF"
     gene            5064..6038
                     /locus_tag="PLM79_11590"
     CDS             5064..6038
                     /locus_tag="PLM79_11590"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOV86998.1"
                     /translation="MKAMILAAGLGTRLRPLTLVRPKPLVPVMGTDLLTYWVHRLHRE
                     GFEAVVVNAFHLHGQMVKAVSERPWPLPVHMCVEPRLLGTAGGLRNALDFFGDEPLAV
                     VNSDVICNVSVADLYEDHLRSGCPASLLVHDFSPFNNVVVAQDGRIVQFGDRPAPPGH
                     DGEPARIMAFTGIYCLTPSILSDVPPGHPRELVPLLTECLSKGMRVRAVTRPPFFWRE
                     IGTLDSYRRVHRELSLREGESPGPLPAGRSFRSEGPAVENSDLSLRGFVVMGRNCRVG
                     KEAVLEDTILWDHVEVKARSSLNNCIVTDGAVVEGNHANEVILGETGL"
     gene            6035..7099
                     /locus_tag="PLM79_11595"
     CDS             6035..7099
                     /locus_tag="PLM79_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOV86999.1"
                     /translation="MKLSAGLEEDLAHLLDEVLPGLWESTDRARVLPMPGDGSDRRFY
                     RIHRGARRMVAVLSPRKEGSKVDENDSYFLIGTHLASRGVPVPRFYWGDPLNGCFLLE
                     DVGDCHFQSLAVRGPSDLEHLYGRALRLLVYLHRHAPEGFDAGFCFDTAVYDPRFVYT
                     RELEYFRTFFLNSLLNLEVPEEELRRDFEDLAEVSGTDERQWVIHRDFQSRNLMVHGG
                     RLRLLDFQGMRYGPPAYDLASLVTDPYVCLPVGIQERLVWEYWKQARRFLGGTRERFW
                     DRYRAVRLSRNVQALAAYAFLGKTKGKNRFLRFVPRAWKQLFAWVKGPCRGRYPRFER
                     RLEQVERQGIPRRVELFRDT"
     gene            complement(7469..9253)
                     /locus_tag="PLM79_11600"
     CDS             complement(7469..9253)
                     /locus_tag="PLM79_11600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOV87000.1"
                     /translation="MDRSVQEVIWNLEDLYAHAGDPRLQEDRARCRKESEAFSRAYRG
                     RVASLNGEELHRAVSRLEVLQERTRRLLSFSNLYFATRTQDSAASALRQAHVELAAAL
                     HRDTLFFEVEWNGIPEAAARALLEHPALASYRHYLQKIRRYQPHTLTEPEERILAEMG
                     PVAASAWGNLFDKVLGRLRFGVEARTQSEVLSDLYHPMREIRRRAAMDLSEGLRSVLP
                     ILGHVFNTVLLDKAITDRIRRYPHWLRERNLENETDDQVVKALIDAAVSRYDLVNRYY
                     RLKRDLLGYEELLDYDRYAPLPHVPTRTFRWDEAAEIVLSAFGRFSREMREIAAFFFD
                     QGWIHAPVLPGKRNGAFSDPTVPSCHPYVLVNFTGTPRDVMTLAHELGHGVHQYLARQ
                     QGLFNSDTPLTMAETASVFGEMLVFRHLLDQTSDSSERLGLLCAKLEDIFATVFRQVA
                     LNRFEDRIHNLRRTSGELDVEEISLCWTSTQEEMFGNSVRLLDHYRTWWAYIPHFIHQ
                     PGYVYAYPFGELLVLSLFRQYQEEESAFVPRYLELLASGGKAPPGELLRPFEIELSDP
                     LFWQKGLDLIRGILEEAEAQAGRLRTEP"
     gene            complement(9307..10749)
                     /locus_tag="PLM79_11605"
     CDS             complement(9307..10749)
                     /locus_tag="PLM79_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOV87001.1"
                     /translation="MNYKRVPVSEVIGIVGEPHVVTSREERYCYGYDASKLKGMPDCV
                     AFPGSTREVSQLLELANRELFAVYPRGAGSGMVGAAVPEDGGLVLVLNRLNRVLEIDP
                     DNMIAVVEPGVVTGAFQKMLAPHGLFYPPDPASLQFSTIGGNIAMCSGGPRAVKYGVT
                     RDYVLGLEVVLPTGRVLHTGTRTMKGVVGYDLTRLLVGSEGTLGVITQAVLRLIPAPE
                     SVRTLLASFASLEDSVRSVCDIVRNRVIPCALELMDQATIQVVENHLSLGLPREAEAL
                     LLIEVDGPDSILDAEAEKIRSICRQNRVSDIETARSPAERDRLWRARRAVSPALGRIK
                     SGKINEDVTVPRTQIPLLIRGIRELAARYDLLIVCFGHAGDGNIHTNIMVDRKDPDEL
                     LRAEKAVRELFRMVLDLGGTLSGEHGVGITKSPFIEWELGREGMETMWAVKKALDPLN
                     ILNPGKMFVPNRAFFACEPSIPGTSGTERR"
     gene            11052..12221
                     /locus_tag="PLM79_11610"
     CDS             11052..12221
                     /locus_tag="PLM79_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOV87002.1"
                     /translation="MEKDPVLLGCEVLAQRAPSWFRSARLGLLSNQASVTSRFEHTRS
                     VLERAGGRFVSLFSPQHGFFAEKQANMIESADSCLESIPVYSLYGAVRQPTREMLRSV
                     DVLLIDVQDVGTRVYTFGTTMGLCMEAAVGSGTKIVVLDRPNPIGGTRAEGNVLKAEY
                     RSFVGRYPIPMRHGLTLGELALFIQAECRVACDLEVVPMQGWNRGDLFPDTGLPWVFP
                     SPNMPSWETAVLYPGMVLLEGCNVSEGRGSTLPFQLFGAPFIDQKRLGEYLHDLHLEG
                     VAFRPICFEPMFDKWQGRTCCGFQIHVTDPRKFRPYNLGLHLLRGFLTTCRDDFQWLP
                     PPYEYERHKLPMDILLGDGCIREAIEAGANIEELEKGWISELREYREKARGCFLY"
     gene            complement(12215..13678)
                     /gene="nadE"
                     /locus_tag="PLM79_11615"
     CDS             complement(12215..13678)
                     /gene="nadE"
                     /locus_tag="PLM79_11615"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOV87003.1"
                     /translation="MISKDPETLVRESVQWLRCRAKRPGTKGFVVELTGGLDSAVTAA
                     LAKLAVGNAVTGLILSCRTAKAQLQDAWATARHMGIPHESLDLGPVWDTFTSVLPRAS
                     KAANEELKDHLRAAALLHFAAVRQCLVLGHGNRSDWEIGNFTKYGDFVGDVFPLFHLL
                     RSQVHDIARLLRIPQRLVKKAPASPALPDLPRGSAGKVTYEMLDAFLEGDLLRVPLPV
                     QDQIRKAQERTAGKRNLFQRFCPHAGPSEESVTEFEDHEAYDKSIEALTLISKAITSD
                     HYLEDILRLIVMVTAEVMNSSVCSLWLLDEKEQVLRLRATQSIDSEYLKDRVLKVGEG
                     IVGKVALEGRPYAVSNVLGDPIYKEKDLARRMKLVSMLSIPMRVKDRVIGVINCYTSH
                     PHAFSDLQMNVLTTVANQAAVAIEKTELMVQTRVIQEELANRKITERAKDLLVKRLSI
                     SGEEAYRWLQKRSMNTRKSMREVSEAVLLTMEGLESQ"
     gene            complement(13713..14378)
                     /gene="lepB"
                     /locus_tag="PLM79_11620"
     CDS             complement(13713..14378)
                     /gene="lepB"
                     /locus_tag="PLM79_11620"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOV87004.1"
                     /translation="MSILSEKEAEKPISKSALREYAEAIIIAVLLALVIRAFVVQAFK
                     IPSGSMKSTLLIGDHILVNKFIYGIKIPFWNKEILHFSDPKRQDIVVFKYPVDPSKDF
                     IKRVIGLPGDTVRIQDKVVYVNDQRLVEPYAVFSDSKIMPAGLSTRDNMGPIIVPLNC
                     LFVMGDNRDESYDSRFWKFVDIKELKGKAFIIYWSWNRDGELTWKPTESYVRWNRIGK
                     WLH"
     gene            14537..15589
                     /gene="pilM"
                     /locus_tag="PLM79_11625"
     CDS             14537..15589
                     /gene="pilM"
                     /locus_tag="PLM79_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOV87005.1"
                     /translation="MFGKKENIVGLDIGSHSIKMVQVSAKNGSLKLLNLGVASVPREA
                     FAEGRVSKADQVARCIQQLASHLKIKDKLVATSISGYEVMIKKIELPMMTEAELENRM
                     QVELGQYIPYNIEEVDVDYQIMDLVKERPNYMDVLLVAAKKESVNDYVSLIRMAGLDP
                     VVVDVDFFALSNAYEAVYGLQEENLVLLDIGANKAIMNITSKGIPIFTRGISIGGNQI
                     THRVLEQFKVGPEEAERLKLGDASDKGSVHDLEDIYVSVVQNWVNEFQRAIDFYYSNF
                     PERKISRILLSGGSSRIPGLNQLFRESLEVPVELFNPLSQLEYDSKVFDSAYLDYVGP
                     QMAVSLGLALRKAKLK"
     gene            15586..>16029
                     /locus_tag="PLM79_11630"
     CDS             15586..>16029
                     /locus_tag="PLM79_11630"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOV87006.1"
                     /translation="MIQINLLPVRTRKKRETGKQFIVLYLLCVLLAGGVIGYLWISKE
                     SEIAALNKRLAQVQAEVKKYAQYEVMLQELKSKKEVVDRKRGVVEDLQRDRDSIARLL
                     AVLSIQVPSEKMWFERLAQTANNVTVDGVALSNEAIVEFMRNLESS"
BASE COUNT         3317 a         4496 c         4870 g         3346 t
ORIGIN      
        1 ggattgaatt ggagccggcg atgggattcg aacccgcgac ctgctgatta cgaatcagct
       61 gctctacccc tgagctacgc cggcccaatg gatgccaggg tctatagcac gcaggtttgc
      121 gggtggtcaa ggagaaaggg gaatgaatct ccccgcttaa cctgttcccc ttgggcagac
      181 ccctgtgtca ggctgacccg gacccccaga gaccttccct caaagcagct tatgaaatga
      241 ttccgtgtaa gtacctacaa tacttgtatg ttcatggttt atggccctaa agacccgtga
      301 tggcacagga attgtataaa gggccggtgg atttgagacg gatacccggg agaggagaga
      361 ggaaagcgct ctttctccct acagtcggcg tttcttgaaa ggatcgtttc ccatgtccaa
      421 atgcatcggt agtctgaaca tttcgctaat gtgcgacgag cacgacacgg ccggcgaatt
      481 ctttaccctg tccgccagca agtacatcca gaagttcatc gggaccagcc tgcctccgga
      541 tatgagggcc ctcatctcct ccggtcagcc cagtcgatgg agaatcggcg atatggatgt
      601 catcatcgat ttcatctgtg cgcaatcggc ccgctccaaa cagagtagtc gagagatgaa
      661 actggtcatc accaatggag ataaggcact cggttagccc gtcctctgcg ccggcatgaa
      721 ccgcgtgcat ccccgcaagc cggcgccggg aggtattccc ttgtacgacc gcaggacaca
      781 ttcaagcgag gagagcgact tccgggactt gtgccccgtg ctcgagttcc cggggtcgat
      841 gccctcgctt gatggcccga gcggccttcc ggcccatgct tcgtcatccc ccatattccc
      901 ctcagccctt tttgtgaaat ccgggaacgt gccgagcgat acccggaacc cagattgatg
      961 cacggcttgc gctcacgagc gggcccggcg gcccgtgttg tctgaacgag gtgatccctg
     1021 tccgtgcagg gtcccgcctt tcttcccacc atgcccgaga ccggtccgtc cggatcggga
     1081 ggatcttttg gggacgggtt ttcaggccaa aatcgggagc actcgctccc ggtgggtccg
     1141 gcagacgagg ccttgatcac ggctttccac catggcaatg tcgccttgac agacgtttga
     1201 aacttcatga taatgccgca ccagcttggg gaagatagag gaataattgt cctctagtat
     1261 ataatggact gcatttatta gatgaaaaat tatatggcaa tatccgtgcc cggagaggtg
     1321 agtgcctatc aatctcctat tatattgagc gacttggctg gtacgattcc cgctttgtcc
     1381 tttttgcgaa agcggggctc ctctgcaacg gattgcaggc tccttgcacg aacggttcgc
     1441 cgggtaaacg gggacccgac aagagcgtgc gcgccctccg gatgctcgcc ttggcgggca
     1501 tttgcgggcc gatgcttttg gaatccctca aacttgggaa cgtgcaacag gactggttgc
     1561 gcagccaaaa tgagtcttgg catcaggcct ctgtaccgct tctccacctc gtcgctcctg
     1621 gagggccggg tctgcaaacg cagggagcat ttgggctccg ggccttcggg aggatgaagc
     1681 ggagacccgc ttgcaagggt tcgataccgg gcaggcacaa tgtccctcac ctcttcagga
     1741 gggtaatgag aaggtcccga atcaggatca agatgcggag tctgcacaat gacacgtaga
     1801 atgaaaacca tcatcgcgga ggcactggta ttcgtcttgg tgctgagcag cgcctccatg
     1861 gcccagaatc tgcaagcggt tccgatcaat ccgtatcccc agcagacaat cccgagtccc
     1921 cccaccatgc ccggaggata tgctcaatcg atgggaactc cccaggccca ggcccagatg
     1981 tctccattgg gaccggctcc cattgctcag cctcaacccc aggtgtacca tcccgggcag
     2041 atcgccccag caccgggcgt tgtgggtgcc acctcctacc ctgcaggcat gcaaacccct
     2101 caaacatttc ctgtccagcc cggagttcct gcaacccagg acatttctca agcggttccg
     2161 tcttcggcct ttgcgaccac ggaggaagag ccgccctccc cctttgagca gtatgtctcg
     2221 ggagtcattc cgtccacgtt gtcctttgat atcaagcaat tcggctacga cgtgttttac
     2281 aagccgagcc ccacgacttt cgttcccctc aaaccggtcc ccgggccttc aatccagcag
     2341 ccgagaggca ctacccagcc ggggggggga ttccccggtg ttccgaccca aggggttccc
     2401 ggcagtgtcg tttctcccca ggcaccgggg gggacgttgc agggttggcc ggggatgatt
     2461 ccctcggggg ttcccggaca acaggtcccc caactgagtc ctgcacagcc gggattgcag
     2521 ccggggggag cttcttccgc cctgtcccag aaggccgccc agcttcaggg actcttgggg
     2581 ccgggagcca acgtacaggc cttggttccc caggcgcagt cgccccaggc ccgagggcag
     2641 gttccgggaa ccgcaacatc cgctgcggcg gcgcttcagt cccagatccc ctcgggcacg
     2701 tccgttcctt atctgcgaaa taatcagtat cctctcttgc agccggtcgc agacgttccc
     2761 gtgggaccgg actatgtcct gggtccgggg gacgaggtgc gcgtggccgt gtggggcagc
     2821 gtggaaggaa cctggttcgt gagcgtggac cgtgacggaa acatcacctt gcccaaggtg
     2881 ggggtcgtcg gggtggcggg ccttacctac caggaactca aggaggtcct tcaaaaacaa
     2941 ctgtccaagt actacacgga cttcgaaatg aacgtcagcc tgggtgcgct tcgctcgatt
     3001 cgcgtctacc tcgtggggaa tgctcgaacc ccgggcgcca tcaccgtatc ttccctttcg
     3061 accatcatca acgcgctcat ggtatccggc ggccccacca agacggggac tctgagaaac
     3121 atcgagctca aaagaaaagg ggagacgatc acccacctgg acgtgtacga cttcctcatt
     3181 cgaggcgaca agaccaatga tacccgactg atgcccgaag acgtgattct gattccgccc
     3241 atcggtccca tcgtgggaat tgcggggaac gtggccaccc ccgccatcta cgaactcaag
     3301 ggcaagacca ggctctccga agggatcaag atggcgggag gggttaccgc tgcggcctat
     3361 ctccagaggg tccaggtgga acgggtatcc aagaagcaaa acaagatcat tcttgatttg
     3421 aacctggaca aggtgaaagg caaagacgac atttacctgg aagacggcga tatcgtcaaa
     3481 gtcttcccca tcaataccgt ggtgaccaac cgggtggtcc ttcaagggag tgtccgaagg
     3541 gcgggcgagt atgagtggaa gccgggcatg cgggtcaaag acctgatccc caacaccgaa
     3601 gccctgttgc ccaataccct gttcacctat gcggccatcg aaagggtggt ccctcccgat
     3661 tatcatccgg agtatgtgac ctttagcctg gaaggcctgc tcattcggaa ggaagaaaag
     3721 gagaacgtca agctcgagcc cttcgacgtg gtgacggtct acgccaagcc ggataagctg
     3781 gagaagcaag tcgtccagat cacgggggcg gtgaatacac cggggcaatt cgagtttcgt
     3841 cccaacatga agctctccga ccttgtgaac attgccggtg gtttgagacc ttacgcattc
     3901 atgaattcgg cggaggtcac ccgggttatt cccacccaac agggtcccaa aaccgaacag
     3961 atctacgtca acctgagcga ggctctggcg ggcaatcctc agcaggacat caccctgatg
     4021 gaaaacgact atcttttcgt ccgatcggtg ccggattggc agatctacaa gaaggtgtac
     4081 gtgtacggtg aggtcaattt ccccggcgag tacgccttta agaagggaga aaccgtctcg
     4141 tcggtgattg aaagagcggg cgggtacacc tcccgagcct atctgcgggg tacggttttc
     4201 acgcgggtga gcaccaagca ggtgcagcag gcccagatca acgaaatggt ggatcgcctg
     4261 gaaagggaga tggcggcggc cgggaccgcg gatctgaccg tggccacgga ccctgtggag
     4321 gcgagggtga ttcagtatca gaacgcccag aagcgggtct tcgtggaagg gttgcgcagg
     4381 gtcgaggcca aggggcgtat ggttgtcaga ctggaccacc cggccaaaat gaagaaaact
     4441 tcctttgaca tccctctgga agaaggcgac atgatcttca ttcccaccaa tccccagacg
     4501 gtgcaggtgc tgggggccgt gtacaaccag accagctttg tgtatgagcc caagagggac
     4561 tacatgtatt acgtcaccaa gaccggggga tacaccagca gtgcggacaa gaaggcggtc
     4621 tatgtgctca agtgcgacgg cacctccgtc aagccgggta gcggtctgac atgggaccat
     4681 gactccaacg agtggcgaac cggcggcagt gtcgtgttgg aaccgggaga caccatcatc
     4741 gtaccggatc agctggacaa gatggggtgg tatgttgcct tgaagggaat cacggaagtg
     4801 atttaccgga tcgccgtggg aaccgggatc ctgttccgcg ctttctaaca acccattggc
     4861 ggaacaagct ccgggagctt gcgaaccagg atttgagggc gggggcggcg cgttcgacct
     4921 cgccccttgc cgttccttcg ccgggccgca ctgggaggcc ttccagtggc ggccgtttta
     4981 ttggggaagg atcgccgggg tcggctcaag ggttatggct ttccggcgta aggtcccgca
     5041 tctcccaggg aggtatgcgc agcatgaaag ccatgattct cgccgcagga ctcgggaccc
     5101 gattgcgacc tctcaccctg gttcgaccca aaccactcgt gccggtgatg ggaacggacc
     5161 tcctgaccta ctgggttcac aggctccacc gggaggggtt tgaggcggta gtcgtcaacg
     5221 catttcatct tcacgggcag atggtgaagg cggtctcgga gcggccctgg cctttgcccg
     5281 tccatatgtg tgtggaaccc aggcttctgg gcactgcggg ggggctgaga aacgccctgg
     5341 atttctttgg agacgaaccc cttgccgttg tcaacagcga tgtgatctgc aacgtgtccg
     5401 ttgcggacct ctacgaagat caccttcgct ccggttgccc cgccagccta cttgttcatg
     5461 acttttcccc cttcaacaac gtggtggtgg cgcaagacgg cagaattgtg caattcggcg
     5521 accggccggc accgccggga cacgatgggg aaccggcccg aatcatggcg tttacgggaa
     5581 tctactgtct caccccgtcg attctctcgg acgttcctcc ggggcatccc cgggaactgg
     5641 tccctttgtt gacggaatgc ctctccaagg ggatgcgggt tcgcgccgtg acccgtccac
     5701 cttttttctg gcgagaaatc gggaccttgg acagctaccg gcgcgtgcac cgggaattgt
     5761 ctctccggga gggggagtcc ccgggaccgt tgcctgcggg acggtctttc cggtctgagg
     5821 gtcctgccgt ggagaattcg gatctttctc tgcgaggatt cgtcgtcatg ggtcggaatt
     5881 gtcgagtggg caaggaggcg gtgctcgagg acaccatcct gtgggaccac gtcgaggtca
     5941 aagcccgcag cagcctgaac aactgcatcg tgaccgacgg ggccgtggtg gaaggaaatc
     6001 acgccaacga ggtgatcctg ggggagacgg gattgtgaag ctgtcggcag gattggaaga
     6061 agacctggca cacctgctcg acgaggtgct gcccggattg tgggaatcca cggaccgggc
     6121 ccgggttttg ccgatgcctg gggacggctc cgaccggagg ttttaccgca ttcaccgagg
     6181 agcgcgaagg atggtcgccg tcctatcgcc ccggaaagag ggatcgaagg tcgacgaaaa
     6241 cgattcctac ttcctcatcg ggactcactt ggcttcccgg ggtgtgcccg tgccccggtt
     6301 ctattggggc gaccctctca acggatgttt tctcctggaa gatgtgggcg attgtcattt
     6361 tcagtctctg gctgttcggg gtccttcaga ccttgagcac ctctacggca gggcgttgag
     6421 gcttctcgtg tacctgcatc ggcacgcccc ggaagggttc gatgccgggt tctgtttcga
     6481 taccgccgtc tacgatcctc ggttcgtgta cacccgggaa ctagagtatt ttcggacttt
     6541 tttcctgaac tcgctgctga accttgaggt ccccgaggaa gagttgcgtc gggatttcga
     6601 ggacttggcg gaagtctcgg gaaccgatga gagacaatgg gtcatccacc gggatttcca
     6661 gagcaggaac ctcatggttc atgggggtcg tttgcggctc ctggacttcc agggaatgcg
     6721 ctacggacct cccgcctatg acctggcatc cctggtgacg gacccttacg tatgtctgcc
     6781 cgtcgggatt caggaaaggc tggtctggga gtactggaag caggcccgcc ggtttctggg
     6841 aggaacccga gagaggtttt gggaccgcta ccgagcggtg cgcctctcga gaaatgttca
     6901 ggcccttgcc gcctacgcct ttctgggaaa aacgaagggg aagaatcgat ttctcaggtt
     6961 cgtcccccgt gcctggaagc agttgtttgc atgggttaag ggcccttgtc ggggcaggta
     7021 tccgaggttt gaacggcgcc ttgaacaggt cgaaaggcaa ggaatcccga ggagggtcga
     7081 actttttcgt gacacatagg cctcaaactg ttatagggca gggtccaata tcaacacagg
     7141 tcggcataga aaggagagga cctccatggc gtcgaaaacg aaacataccc ggctcgtacg
     7201 gaagcgcaag cacagaccga acaaaatcaa cgtcaaaacg gaccggcaac ggatcgtgaa
     7261 aaatttggaa gttctggcca aggtcaccgg caccgcttcc tgacccaacc gcgaaggacc
     7321 tgcccgacca tcgactccgc cccggccccg agacaggaaa cgatccgcgg cgacggacgg
     7381 aaagacgggt ccgccgttcc cggaacaccc cgacgggagc ggggtcgcgg gatcatttcc
     7441 ccgggacccc gttttgccgt cgccgggatc agggttcggt ccggagcctg cctgcttgcg
     7501 cctcggcttc ttcgagaatt ccacggatca aatccagacc cttttgccag aaaagaggat
     7561 cggacaactc gatctcgaag ggtcggagca actccccagg cggggccttt cctcccgaag
     7621 ccagcagttc caggtagcgg ggcacgaaag cgctctcttc ttcctggtat tgtcggaaga
     7681 gggagagtac caggagttct ccgaaaggat acgcgtacac atatcccggc tgatggatga
     7741 agtggggaat gtaagcccac caggtcctgt agtgatcgag cagccgcact gagttgccga
     7801 acatctcctc ttgggtcgag gtccagcaaa gggagatttc ttccacgtcc agttcgcccg
     7861 aagtgcggcg caggttgtgg attcgatctt caaaacggtt cagggcaacc tgccggaaga
     7921 cggtggcgaa gatgtcttcc aacttggcgc agagcagtcc cagtctttcc gaagaatccg
     7981 aagtctgatc cagtagatgc ctgaatacca gcatttctcc gaagaccgat gcggtttccg
     8041 ccatggtgag gggagtgtcg ctgttgaaga gaccttgttg cctggccaga tactggtgaa
     8101 ccccgtgccc cagttcatgg gcgagggtca tgacgtcccg gggggtgccg gtgaagttca
     8161 ccaggacata cgggtggcag gaaggaacgg ttgggtcgga aaaggcaccg tttcgcttcc
     8221 ccggaagaac cggggcatga atccatcctt ggtcaaaaaa gaaggcggca atttcccgca
     8281 tctcccggga aaaccggccg aaggccgaga ggacaatctc tgcggcctcg tcccaccgga
     8341 aggtgcgggt cgggacgtgg ggaagggggg cataccggtc gtaatccaag agttcctcat
     8401 acccgagaag gtcgcgtttc agtcggtaat aacggttcac caggtcgtac cgggacactg
     8461 cggcatcgat cagggctttc accacttggt cgtctgtttc gttttccagg tttcgttccc
     8521 ggagccagtg gggatagcgg cgaatgcggt cggtgatggc cttgtccagc aggaccgtgt
     8581 tgaagacatg gcccagaatc ggcaggacgc tcctcaatcc ttcgctcaga tccatggccg
     8641 cccggcgccg gatctcgcgc atcggatggt aaagatcgct gaggacctcg gattgggtcc
     8701 ttgcctcgac gccgaacctc aaacggccca ggaccttgtc gaagaggttg ccccaggcgg
     8761 aagccgccac gggtcccatt tccgccagga tgcgctcttc gggttccgtg agggtgtggg
     8821 gctggtagcg acggatcttt tggagataat ggcggtagga agccagggcc ggatgttcca
     8881 agagtgcccg agccgccgcc tcgggaatcc cgttccattc gacctcgaaa aaaagagtgt
     8941 ctcggtgcag ggcggcggca agctcgacgt gggcctgccg cagggcgctt gccgcggagt
     9001 cctgggtccg ggtcgcgaaa tacaggttgg aaaacgaaag cagcctgcgg gtgcgctcct
     9061 ggaggacctc caaacggctg acggcccggt ggagttcttc cccattcaga gatgccacac
     9121 ggccgcgata ggctcgggaa aaagcctccg attccttccg gcaccgggcc cggtcttcct
     9181 gcaggcgggg atccccggca tgtgcgtaga gatcctcgag gttccagatc acctcttgca
     9241 ccgaacggtc cattccgttc ccttccttga tcgccatagg gaactcgttt cccggggaga
     9301 ggaagcctat cgtctttccg taccggatgt tccggggatg ctgggctcac aggcaaaaaa
     9361 ggcgcggttg ggaacgaaca tcttcccggg gttcaggatg ttcagcgggt ccagggcctt
     9421 cttgacggcc cacatggtct ccatcccctc gcgtcccaat tcccactcga tgaaggggga
     9481 cttggtgatg cccactccgt gttctcctga aagagtgccc ccgaggtcca gaaccatccg
     9541 gaaaagctcc cggacggcct tctcggcccg caggagttca tccgggtcct ttcggtccac
     9601 catgatattc gtgtggatgt tgccgtcccc ggcatgacca aaacagacga tgagaaggtc
     9661 gtacctggcg gccaattccc ggatgccccg gatgagcagg gggatctgcg ttcggggcac
     9721 ggtcacgtcc tcgttgatct tccccgactt gatgcggccc agggccgggg agacggcacg
     9781 gcgtgcccgc cacagtcgat cccgctcggc cggggagcgg gccgtctcga tgtcggatac
     9841 gcggttttgc ctgcagatgc tccgaatttt ctccgcttcg gcgtccagga tggaatccgg
     9901 tccgtccacc tcgatcagga gtaaggcctc cgcttcccgg ggcaggccca gggacaggtg
     9961 gttttccacc acctggatgg tcgcctgatc catcagttca agtgcacagg gaatcacccg
    10021 gttgcggaca atatcgcaca ccgagcgcac ggaatcctcg agacttgcaa aagaggccaa
    10081 gagggtccgc acgctctccg gggcgggaat cagcctcaga acggcctgag tgatgacgcc
    10141 gagggtccct tcggaaccca ccaggaggcg ggtcagatcg tatcccacca ctcccttcat
    10201 ggttcgagtc cccgtgtgga gtacccttcc cgtgggcaag acgacctcga gtcccaacac
    10261 gtagtcccgg gtgacgccgt acttcacggc cctggggcct ccgctgcaca tggcgatgtt
    10321 tcccccgatg gtggaaaact ggaggctggc gggatcggga ggataaaaca acccgtgggg
    10381 cgccaacatc ttctgaaagg cccccgtaac cacccccggc tccaccacgg cgatcatatt
    10441 gtccgggtcg atctccagca cgcggttcaa ccggttgagc accaggacca ggcccccgtc
    10501 ttcggggacg gcggccccca ccatcccgct tcccgcaccc cgggggtaga cggcaaagag
    10561 ctcccggttg gccagttcca ggagttgaga gacttcccgg gtgcttcccg gaaaggcgac
    10621 gcaatcgggc attcctttga gtttgctggc gtcgtacccg tagcagtagc gttcttcccg
    10681 gcttgtcacc acatggggtt cacccacgat tccaatgact tccgaaacgg ggacgcgttt
    10741 gtagttcatc gtttcaccct gccctgccgg agcccgttgg gatgagtttg aacggcttca
    10801 cgatgggaag aggcgatctc ccggacgagg gccctcatgc ccaaaaaccc gtgccggaag
    10861 gtcgcccgac acgacccagg gaacggcatc caaatcgctt ccgactatag tgaagcttgc
    10921 tttctcttgc aacctccgtc cggttgcgtt tgaatgtcgg caaagaacgg gctgaggaga
    10981 gggtgcattg tcgcaacggc gtggaaagcc gagatggagc tgcggcgggg aaatccggag
    11041 ggattggaac ggtggagaaa gatccggtgc tgttgggatg tgaagtcttg gcgcaaaggg
    11101 cgccttcgtg gtttcgttcg gcgcggctgg ggttgctgtc caaccaggcg tccgtcacaa
    11161 gcagattcga gcatacccgt tcggtgttgg agcgggcggg aggacgtttt gtgagcctgt
    11221 tttctcccca gcacggcttt ttcgccgaga agcaggccaa catgatcgag tcggcggact
    11281 cgtgcctgga atccattccc gtttacagcc tctacggtgc ggtccgtcag cccacccggg
    11341 aaatgcttcg gtccgtcgac gtgttgttga tcgacgtgca ggacgtgggg actcgtgtgt
    11401 atacctttgg gacgaccatg gggctctgca tggaggcggc ggtcgggagc gggacgaaaa
    11461 tcgtggtcct ggaccgcccc aaccccatcg gcgggactcg tgcggagggc aatgttctca
    11521 aggccgagta ccgatccttc gtgggtcgat acccgattcc catgcgccac ggattgacct
    11581 tgggggaact ggcgttgttc attcaagcgg agtgtcgggt tgcctgcgac ctcgaggtcg
    11641 tccccatgca aggctggaat cggggggacc ttttcccgga caccggactg ccctgggtct
    11701 ttccttcccc caacatgccc tcctgggaga ccgccgtcct ttaccccggg atggtgctcc
    11761 tggagggctg caatgtcagc gagggcagag ggtctacgct gccttttcag cttttcggag
    11821 cacccttcat cgaccagaaa cgcctgggcg agtatctcca cgacctgcat ctggaggggg
    11881 tggcgttccg tcccatctgc ttcgagccca tgttcgacaa gtggcagggg agaacctgct
    11941 gcggtttcca gatccacgtg acggaccctc gcaaattccg gccctataac ctgggactac
    12001 accttcttcg gggatttctc accacctgca gggacgactt ccagtggctg cctcctccat
    12061 acgaatacga gcggcataag ttgcccatgg acattctgtt gggcgacggg tgcatccggg
    12121 aagccatcga ggcgggggca aacatcgagg aactcgaaaa ggggtggatc tccgagttgc
    12181 gggagtatcg ggagaaggct cggggctgtt tcctctattg actctccagc ccctccatgg
    12241 tcaggagaac ggcctcggag acttccctca tggattttcg ggtgttcatg ctccttttct
    12301 ggagccaccg gtaggcctct tccccggaaa tggacaggcg cttcaccagc agatccttgg
    12361 ctcgttcggt gatctttcgg ttggccagct cttcctgaat gacccgcgtc tggaccatga
    12421 gttccgtctt ctcgatggct accgccgctt gattggccac ggtggtgagc acgttcatct
    12481 gaagatcgct gaaagcatgg gggtgggagg tgtagcagtt gatgacaccg atgacccggt
    12541 ccttgacccg catggggatg ctcaacatgg acacgagttt catgcgccgg gccaggtcct
    12601 tttctttgta gatgggatct cccaggacat tggaaaccgc gtaaggacgg ccctcgaggg
    12661 cgactttccc cacaatgccc tcccccacct tgagaacgcg gtccttcagg tattcgctgt
    12721 cgatggattg ggttgccctg agccgcagga cttgttcctt ctcgtccagc agccataagg
    12781 aacagacgct cgagttcatg acctccgccg tgaccattac gatgaggcgc aggatgtctt
    12841 ccaggtagtg gtccgaggtg atggccttgc tgatgagggt gagggcctcg atactcttgt
    12901 cgtaggcttc gtgatcctcg aattccgtca ccgattcttc cgacggaccg gcgtgcgggc
    12961 aaaagcgctg gaagaggttc cgcttacctg ccgtgcgctc ctgggctttc cggatctggt
    13021 cctgaacggg gagtgggacc cgaagcaggt ccccttccag gaaggcgtcg agcatctcgt
    13081 aggtcacttt ccccgcggag ccccggggaa gatccggaag ggcgggggat gccggagcct
    13141 tcttgacaag gcgctggggg atccgaagca gtcgggcgat gtcatggacc tggcttctca
    13201 ggaggtggaa aaggggaaag acatccccga caaaatcccc gtacttggtg aagtttccga
    13261 tttcccagtc gctgcgattc ccgtgaccca ggaccaggca ttgacggacg gctgcaaagt
    13321 gaagaagagc tgccgcacgg aggtggtcct tgagctcttc gtttgcagcc ttggaggctc
    13381 gaggaagcac ggaagtgaat gtgtcccata ccgggcccag atccagggat tcatggggta
    13441 tccccatgtg tcgagctgtc gcccacgcgt cttgaagctg ggccttggcc gtacggcagg
    13501 aaaggatcag cccggtaacg gcgttgccca cggcgagttt tgcaagagcg gccgtcacgg
    13561 ccgaatccaa tccccccgta agctcaacca caaacccttt ggttcccggg cgttttgctc
    13621 tgcagcggag ccattggacg gattctctta ccagggtctc ggggtctttg gaaatcatat
    13681 ccgttccatc ccaggtcccg ggaacgaaga ggtcaatgga gccacttgcc gatgcggttc
    13741 caacggacat agctttcagt gggtttccag gtaagctcgc cgtcccgatt ccaggaccaa
    13801 tagataatga aggccttccc cttaagttcc ttgatgtcca cgaatttcca aaagcggctg
    13861 tcgtagctct cgtcccggtt gtcccccatg acgaagagac aattgagagg aacgattatg
    13921 ggacccatat tgtcccgggt gctgagaccg gccggcatga ttttgctgtc gctaaaaacg
    13981 gcatagggtt caaccagcct ttggtcgttg acgtagacga ctttatcctg tattcgtacc
    14041 gtatcaccgg gtaagccaat gactcttttg ataaaatctt tggagggatc cacgggatat
    14101 ttgaatacga cgatgtcctg gcgtttgggg tccgaaaagt ggaggatctc cttgttccaa
    14161 aaggggatct ttatcccgta aatgaatttg ttgaccagaa tatggtcccc gatgagcaga
    14221 gtggatttca tggagccgga ggggatcttg aaggcctgga ccacaaaagc tcggatcaca
    14281 agggccagca ggacggcgat gatgatggcc tcggcatatt ccctaagagc gctcttggag
    14341 atgggttttt cagcttcttt ttcagagaga atggacaacg ggtttcctcc tttgaaggcg
    14401 acataatccg tgcgaatgca ggcgtcgctt actgtaataa gtttcttgca agttttcaag
    14461 gaaggatggt agactaggaa cgtcttgaaa tgtcaatgga aaccttgcac ttactcgtca
    14521 ggggcgggcc cactctatgt tcggaaaaaa agaaaacatt gtcggtttgg atattgggtc
    14581 ccacagcata aagatggtcc aggtgagtgc gaaaaacggg tcgctcaagc tgttgaacct
    14641 cggagttgcc tcggttccgc gcgaagcctt tgctgaggga agggtgagca aggccgatca
    14701 ggttgccagg tgtatccagc agttggcaag ccacctcaag atcaaagaca agctggtggc
    14761 aacttccatt tccggctatg aggtgatgat taagaagatc gaattgccca tgatgacgga
    14821 ggcggaactg gaaaaccgga tgcaggtgga gttgggccaa tatatcccat acaacataga
    14881 agaggtggac gtcgattatc agatcatgga ccttgtcaag gaacgcccca attacatgga
    14941 tgtcctgctg gtggccgcca agaaggagtc ggtcaacgat tatgtaagcc tgatccgaat
    15001 ggccggattg gaccctgtag tggtggatgt ggattttttc gccctgagca atgcttatga
    15061 agcggtttat gggctacagg aagaaaacct tgtattgttg gacattggcg caaataaagc
    15121 tattatgaat atcacctcta aaggaattcc tattttcact cgagggatat ccattggggg
    15181 caaccagatc acccaccggg tcttggagca gttcaaagtc ggccccgagg aagcggagcg
    15241 cctgaaactg ggagacgcct ccgacaaagg gtccgtccat gatctggagg atatttacgt
    15301 ctcggtggtt caaaactggg tgaatgaatt tcagcgtgct attgattttt attacagtaa
    15361 ttttccggaa cggaagatca gtcgaattct cctgagcggg ggctccagca gaattcccgg
    15421 cctgaatcaa ttgtttcggg agagtctgga ggtacccgta gaactgttca accccctttc
    15481 tcagctcgaa tatgattcca aggtattcga ttccgcgtac ctggattacg ttggaccgca
    15541 aatggccgta tctttgggac tggctttaag aaaggctaag ttgaaatgat tcaaatcaat
    15601 ttgctcccag tccgcaccag gaagaagagg gaaacgggaa aacagttcat cgtcctgtac
    15661 cttctttgcg ttctgttggc aggcggcgtc atcgggtatc tgtggatctc caaggagagc
    15721 gagattgccg cactcaacaa gcgcctggct caagtgcagg cggaagtcaa gaagtacgcc
    15781 cagtacgagg tcatgctgca ggagctgaaa agcaagaaag aggtcgtgga tcggaaacga
    15841 ggagtggtgg aagatctgca gagggaccga gacagcattg ctcgcctgct tgccgtcctc
    15901 agcatccagg tcccttcgga aaagatgtgg tttgagaggc tggctcaaac ggcaaacaac
    15961 gttaccgtgg acggtgtggc tctgagcaac gaggccatcg tggaattcat gcgcaatctg
    16021 gaatcgtcg
//
LOCUS       DAOOVC010000089        16024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2450_length_16024_cov_6.264826, whole genome shotgun sequence.
ACCESSION   DAOOVC010000089 DAOOVC010000000
VERSION     DAOOVC010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 16024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16024
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2450_length_16024_cov_6.264826"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLM79_11635"
     CDS             <1..555
                     /locus_tag="PLM79_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87007.1"
                     /translation="VRQALPIPTLFVLPWSPPALPASTLQRLVQGRNMAVAAPVAGAE
                     EMGSSITLLAPDGPVLLPSNAVSVKADFGPSRLALARLDALRHPELVTALSKQGCDFV
                     VTGTGTLTPDDLLLLGVRTIERTAVAVAGTNGAAVFEPPVGHAPWGETLLHGRGTCVA
                     RLHTNSLRNKRFHDRVDMELLLRR"
     gene            952..1692
                     /locus_tag="PLM79_11640"
     CDS             952..1692
                     /locus_tag="PLM79_11640"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOV87008.1"
                     /translation="MEAVRKGAVHHSPTVFFRANDVGAGGRAFEALCRLFRRLQVPLA
                     MAVVPAWLSPVRKRQLFQAAPLEEALWGWHQHGWRHVNWQKTGDKSEFGEHRPYEKQW
                     RDIWQGRRKMKECFRGFMADVFTPPWDRLSPATLRVLDQLGFKGVSMGAAVPKGTKSA
                     SALRYFRTQLNLHERDSKDAASDFHDLLEEITALLSRKKLIGITVHHHRMTSFAFEFL
                     EKLILLLDQQADCRFVGFRDLLESDEHE"
     gene            1685..2962
                     /locus_tag="PLM79_11645"
     CDS             1685..2962
                     /locus_tag="PLM79_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87009.1"
                     /translation="MSKKPSLVFADLEGKIYDWSELEMVGRGGKGFQRLETGDWIPLP
                     EGSELFLLPRRYPVGYHPQRRTFETLSRNPFDRGKPVQAVAAFVAPAHTQLFLAAYQT
                     LPEAPLLPLFAYTAVGWLDGRFVAAALRIDSDQRQDFRHFDPDRIRRNARRRMERDRS
                     NRLVQHLGKCALSYGCPAARNLFLSRWEAPLPTSPVCNARCLGCISLQERTGLKATQE
                     RIRFVPTPEEIAEVAVPHLKEAERAVVSFGQGCEGEPLLQGPTLEKSVKLMRTATPRG
                     TIHLNTNGSLPRRVAKLCEEGLDSIRVSLNSCRPGPYQRYFRPRDYSFESLIESLKIV
                     KSRGGFASINYFVHPGFTDSEEEWSALRRFLDETRINLIQMRNLNIDPEWYEKSLGLP
                     EDDRSMGMRRLIDRLRSSYPDVRLGYFNPPVRG"
     gene            2985..4445
                     /gene="lhgO"
                     /locus_tag="PLM79_11650"
     CDS             2985..4445
                     /gene="lhgO"
                     /locus_tag="PLM79_11650"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HOV87010.1"
                     /translation="MTKPFDIAFVGAGIVGTASAMKMVRANPGLRVAVIEKEDAPGRH
                     QTGHNSGVIHSGLYYRPGSLKARLCVEGARQLVEFCREKGVPFELCGKVVLAVREDEI
                     MRLHDLHTRGRANGVPDLRILHPDQVRDIEPHARSLLGLHVPSTGIVDFGRVTQAYVR
                     EFLSGGGSLFLGHRVAAVLRDSSLIHLETGRGRVSARSLVNCAGLYSDRVARMAGLNP
                     PCQIVPFRGEYYRIRSDRSRLVRNLIYPVPDPRFPFLGVHFTRMLDGTVEAGPNAVLA
                     YAREAYRKDRVVWSELLETLSYPGFRRMAGRYWRAGWHEILRSYSRTLFARALRNLVP
                     EIRREDLLPGGAGVRAQALGPDGALLDDFVLLKEERMVHVLNAPSPAATSWPLRTLSC
                     RRRPRSFEPETAAFRAPTGPSRHAVAVEGTAWPCLDGWAGQTAGTAPSSRPRPCPPFR
                     LPHERTHRIRHPCVGLRAGGPSRCCLRRGWETGAWR"
     gene            5453..7159
                     /locus_tag="PLM79_11655"
     CDS             5453..7159
                     /locus_tag="PLM79_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOV87011.1"
                     /translation="MSQGSLLEAFRTELNLQEDQTKQLQSSYFVEGLPLMDSFEQIAV
                     TDEHRVLRVLARHLDLPLLERQDYPEKPVLLEGVSMFFLRKHSVLPIHMDSGRVRVVV
                     NNPLNLPMLNTLTQYFLGMELQLCLGLREEIRTAIDRLYGTAAKEAEALSRSAELGSL
                     NGDSFEEDLEHLKGLAQEAPIVRMVNVLISRALDMRASDIHFEPFDRGFQVRCRVDGV
                     LFDLDRPPKSMQAAIISRLKLMANLNIAERRLPQDGKIKLKFGNREVDVRVSTVPTIF
                     GESLVLRLLAQESVDYSLASVGMDPQQLGFIEQLIERPFGMILVTGPTGSGKTTTLYG
                     VLRKLNSVTRKIITVEDPVEYQINGINQIQVKPQIDLTFANALRSLVRQDPDVLLIGE
                     IRDKETADIAIESALTGHLVLSTLHTNDAPGAITRLRDLGTESFLIADSLLAILAQRL
                     VRILCPHCKEPYPAREADIQRLKEVLEEVPHTTLYHNKGCDRCGFTGFRGRTAIFELL
                     PISETVRSAIVGGKDAGSIAQLGYREQYRPLLHHGYRKALDGVTTLSEVLRVTSLLSW
                     TG"
     gene            7219..8406
                     /locus_tag="PLM79_11660"
     CDS             7219..8406
                     /locus_tag="PLM79_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOV87012.1"
                     /translation="MAYYQYHARDTMGGTHRGVMEAASEVEAAGKLKTEKLYPVKIKP
                     VRSQRPRRVPEEHIIRFFYDLSDLFYAGLPVDRALALISTNQTHKTFRQVVQGLLGDV
                     QGGSDLSGAMGKYRDVFGNLSDHMVRAGEAGGTLGPILKRLAEYLEQRRTFRQSMISS
                     MIYPLILLGVSAVSMVILLVYVIPKFARIFHDLNQKVPFLTNLMLEAGMFLKDYGWIL
                     PLAFGAVFFGGRALYRKPKVRKKVDKILFRMPLTRYLILHSELTRFCRTLGTMIEAGV
                     PLLRALSLGEQLVINSVLREALVPLHREIKAGHSMSNFFRSHSMFPVRVGTMLRIAEE
                     QGNLGQGLMGLGDHFEKELQRTLQRIMALMEPAVIIATGLMIGTIVLSMFTAIFGINE
                     IQF"
     gene            8409..8876
                     /locus_tag="PLM79_11665"
     CDS             8409..8876
                     /locus_tag="PLM79_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV87013.1"
                     /translation="MAPSPLTRLKSRGFTLVELMVVLVILAITVGLVLPRVGGGWKRM
                     EEREFVQDFVRTLRSARLRAMNTGTIAIFRIRGPERLYGFEVPPETAIPLNVDIYADR
                     LEQDPFTGDHVVLFYPDGSLSGSDLQILFDQQRSFHIFIHPLFGTVQVTRTEP"
     gene            8873..9382
                     /locus_tag="PLM79_11670"
     CDS             8873..9382
                     /locus_tag="PLM79_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV87014.1"
                     /translation="MKQPAAANPPESAKSPSPGREDLHLRPGRTARTRPARGRRLIAG
                     ADSRKGFTLLEVMVSLTIGLILLGGLMGLISVSLHYGQRLRHKSEVQPVLEAAAQTIL
                     AFPEKAGEGALVMESFPDKPAVEIRLARVFEKDETTPPTLGELYRVQLLYRGQMLEFS
                     ILVPTPPTQ"
     gene            9410..10132
                     /locus_tag="PLM79_11675"
     CDS             9410..10132
                     /locus_tag="PLM79_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOV87015.1"
                     /translation="MRRLRQKPSNFPANNRGFTLVELVLATLISTLVIGILSVSLAFS
                     LRVWERQQNQKPKDILSLLNLLKWQLAQFDPEPIALGSETEPIFRGDETSLAFATDYS
                     TKAICKGVPAVVRYVFVPGEQAVYYAEVPLHPYDPDGIKRFLELRPQKHRGWPPFHAV
                     PVAQFSLAYIAKDSVSEQAGPWEGEESVPAGVVVSWNDGSNDVATLRFVVPNSLFHRK
                     PQSSLQPTGPGRLQGTGRRKLF"
     gene            10132..11043
                     /locus_tag="PLM79_11680"
     CDS             10132..11043
                     /locus_tag="PLM79_11680"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOV87016.1"
                     /translation="MRNQRGVVIVAVLWICSLLLWFALQIGTETRLRGEEQVHEIRRS
                     QALHLAIGGAYEALARMGQPLPTGLEDAFGKSVRGKNTLRRDSAEENWQPDGTGHIVE
                     YQTGEALVFIEPEGRKVNVNRANREQLKMVFERTGLAEDRIEQLADVTADFLDNDDLP
                     RLHGGEKDYYQRLGLPYPPFNGPLLSLDQMLLIPGITQDLFFGYGPRENEDRNEASPP
                     PVPGLPGNNSLFRMFTVYGTNTTLPSGELEEEIQEKIETWRPGEIYRLLSCGKAYTGP
                     PVVVLWLIVRFQPQLPGGYEVLYRKIL"
     gene            11071..12672
                     /locus_tag="PLM79_11685"
     CDS             11071..12672
                     /locus_tag="PLM79_11685"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOV87017.1"
                     /translation="MLRGLVKRRPRQLVAVYVEPERIEVLRARRHWRSWRIESTEQFP
                     VPRGEGLYDFLQRLALRPRDKKETALVLFIPRTFYNLHREHYPSTLRDQLQEAIAFDW
                     QENVFVEEEQSLHFCGPPVGVDHQISVPIFSMQRDLYDKLYQALGAALFDVFVVIPSA
                     LVYKALLPSIVPRDEEESSLEIVGRFLEPHRLEIHRYLKGEFLDSFLVSRNLDTLRLF
                     REALACIGNGQCPPERSIHLLCSDGERAEDYVLAWKDENLPMQIHKSPEPLLLHWVNY
                     LLQQDKVQTFNAPLLLKPWKVPKVVWPMLALAAMFAAFAAYQVHSLGQMQQSSKRIKR
                     QIAQLETQWKPVEQLQTRVGKFEEDRKTLNEFNQEGYPLLEMLSLLTQITPEDTWLNY
                     FSIRKGQMVLRGESKSAIKYLSELSKVEGFGDVRFSSPVTRNPSSDLERFNVQVQIDV
                     EKLKKTTDSLGIQTLDTPAADAGGESVAESPEPATAQIPPLRVPEKEQDEEETEDAEE
                     AEEAEVTTADTSESEEEDPEEEQGR"
     gene            12690..13277
                     /gene="pilO"
                     /locus_tag="PLM79_11690"
     CDS             12690..13277
                     /gene="pilO"
                     /locus_tag="PLM79_11690"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOV87018.1"
                     /translation="MKYPKISWSEIQKYRLASLGIAATILALVVVHVFWLAPVLSEKE
                     ILENRLKQQQDLMQKYEDKLKQAHSIRENLVKKEAELREMQKRLFRGNDPYQLAASLG
                     ETFSPKGGGKAKLEIKTYQVIASKEYGLYQEVHLRFNLMTTVEGLHFFLDRVGKFQTA
                     ILVKEINIQKIQKKGGPDLVVNVILAALMEKGEKS"
     gene            13428..15896
                     /gene="gspD"
                     /locus_tag="PLM79_11695"
     CDS             13428..15896
                     /gene="gspD"
                     /locus_tag="PLM79_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOV87019.1"
                     /translation="MKWYRAFLSLLLAAMLAACAQPSPLGRGQADTGIRAPRPPASAA
                     RMPREISTDLSKSADATSADAAKKKPVLPSVTAKPSYGLMSPPSDQKPEKLPLSDKEK
                     QKVVLNFEKADVAEVTNQIFGDYLKLNYVLDPGLQGRLSFYLEGEFTKEELYQMILKA
                     YEANNISIASRKGIFYIQPLQKGSSSSLPLATKALLQDEKAGARPVIVLYRLQFMEAK
                     QALNVIKTFLTPGRPVASDNLTNSLIFVEDAENARSILDVLKALDINILREVSMEIVP
                     VQGMSPMDAAQGMEALVSKMSLFKESTVKSNVAFIPLPNFGGVLVLAQNPEVLRIAKS
                     WLTALDVQGKEAGEQVYVYFVQNGLAGDIADILNQVFGLGGTTGGRRLQQQVVRSSRQ
                     FGGTGQGLGGTGGFSGRTFGGTSTGTGGFGSSGGFSSTGRTSGFSSAAQSGDGSGTST
                     GTSGTSSFGTGAGAAARRARVPGGLGAPGQTLPNILSGEAVIIPDEVNNAIVIRANAN
                     DYAKIRKTIETLDILPRAVLIEVMIAEVTLNKELQYGLQWFFQDIGIKIGGEEGKFTI
                     AKVNPGAGTSGTGTDGTGGSNVNPDNTNRNRRTFLNLAPSATGGLSTFWGTLPHNGVR
                     DVAVLLDLLGSKTTVNVLSTPTLLATDNQQASITVGGREPIQTGQTLLEGGVSTNNIF
                     TTIQYEETGIILNVTPHINAGGLVRLEVEQTIRNVAEQTTQGINSPRFTERNVKTTLL
                     AQDGSTVVIGGIIEDRRSDGKTGIPVLQDMPIIWPLFSTTDKKMNRTELIIAITPHVV
                     DHRESDVTREFLGKLKQLKKRIEN"
BASE COUNT         3196 a         5030 c         4725 g         3073 t
ORIGIN      
        1 gttcgtcagg ccctgcccat tcccaccttg ttcgtgctcc cctggagtcc acccgccctc
       61 cctgcgagca ctctccaaag gctggtgcag ggccgcaaca tggccgtcgc ggcccccgtg
      121 gcgggagcgg aggaaatggg atcgtccatc actctccttg ccccggacgg ccccgtcctc
      181 ctgccttcga acgccgtttc cgtcaaggcg gacttcggcc cttcccgcct ggccctggct
      241 cgcctggatg ccttgcgtca ccctgaactg gtgacggccc tatccaagca ggggtgcgat
      301 ttcgtggtga cgggaacagg aacgctcacc ccggacgatt tgctcctgct gggggtcagg
      361 accatcgagc ggacagcggt ggcggtggca gggaccaacg gggcggccgt cttcgaaccc
      421 cccgtcggcc atgccccttg gggagaaacc ttgctgcacg gtcggggaac ctgcgtggcg
      481 cggctccaca cgaactcctt gaggaacaag cgattccatg accgggtgga catggagctg
      541 cttctgcggc gatgagcccg aatcctcgtt tttcccgccg catgcggcct gcccattcat
      601 tgcccttctg ccgatcccgg ctacggagcg caaatcatgg ttttccaagg ccaaaggtac
      661 gcacttgacg aaagagggtc ggttcgctac cctaggaact acctcgcagt gggggaattt
      721 tccaaacgtt gtaccgatct catcctttct gtaaacaatg tgagcgtggg gtttaaagac
      781 ttgctgttcg tgactgtacc gaccgggtgt ccgggatgaa aaggaatcgc tgccattttt
      841 agaggtgcta tcgccaagac ttcggacgtc gtcggaccga ctcctcccat tcggccgcgc
      901 cagttctcgg ccctgtggca cagtgccccg gatcgttggg aggaactcct cctggaagcc
      961 gtccggaagg gtgccgtcca tcacagtccg accgtttttt tccgcgccaa cgacgtgggg
     1021 gccgggggcc gcgcttttga ggccctgtgc cgactcttcc ggcgacttca ggtccccctg
     1081 gccatggccg tggtgcccgc ctggctgagt cccgtacgaa aacggcagct gttccaggcg
     1141 gcccccctgg aggaggccct ttggggatgg caccagcacg ggtggcgtca cgtgaactgg
     1201 caaaagacgg gggacaagtc cgaattcgga gagcatcggc cttacgaaaa gcaatggcgg
     1261 gacatctggc agggacggcg aaagatgaag gagtgttttc gaggcttcat ggccgacgtg
     1321 ttcactcccc cctgggaccg actctccccg gctaccctgc gggtcctgga ccagttgggg
     1381 ttcaagggcg tctccatggg ggcggccgtc cccaagggca cgaagtccgc ttcggcgttg
     1441 cgatacttcc gcacccagct gaacctccac gaaagggact ccaaggatgc cgcgtccgac
     1501 tttcatgacc tgctggaaga aatcacggca ctcctcagca ggaagaaact catcgggatt
     1561 accgtccatc accatcgcat gacctcgttt gcctttgagt tcctcgagaa gctcatcctc
     1621 cttctggacc aacaggcgga ctgccgcttc gtcggcttcc gagacctcct ggaatccgac
     1681 gagcatgagt aagaagccgt ccctggtatt tgcggatctc gagggcaaga tctacgactg
     1741 gtcggaactg gagatggtcg gcagaggggg aaaggggttt caaagactcg agacgggtga
     1801 ttggattccc ctgcccgagg gcagcgagtt gtttctgctc ccccggcgtt accccgtggg
     1861 gtatcatcct caacggcgaa ccttcgaaac cctctcccgt aaccctttcg accgcgggaa
     1921 acccgttcag gcggtggcgg ccttcgtcgc cccggcccac acccagctct tcctggccgc
     1981 ctaccagacc cttcccgaag cccccctgct cccgcttttc gcctacaccg cggtgggctg
     2041 gctcgatggc cgtttcgtgg cggccgccct gcgaatcgat tccgaccagc ggcaggactt
     2101 ccgtcatttc gacccggacc ggatccgtcg aaacgcgcgg cggcgcatgg aaagggaccg
     2161 cagcaaccgc ctggtgcagc acctgggcaa gtgcgccctc tcctacggct gtcccgccgc
     2221 aagaaatctc ttcctctccc ggtgggaagc ccctcttccc acctcccccg tatgcaacgc
     2281 ccgttgcctg ggctgcatca gcctgcagga acgcacgggt ctgaaggcta cccaggagcg
     2341 catccgtttc gttcccaccc ctgaagaaat tgccgaggtg gccgttcctc atctgaagga
     2401 ggcggaaaga gccgtcgtgt ccttcgggca gggctgcgag ggagaacccc tgctccaggg
     2461 accgaccctg gagaaatccg tgaaattgat gcgaacggcc acgccacgag gaaccatcca
     2521 cctcaacacc aacggaagcc tcccccggcg ggtggccaaa ctgtgcgagg aaggcctgga
     2581 cagcatacgc gtcagcctca acagctgccg cccgggacct taccaacgct acttccggcc
     2641 tcgggactat tcttttgaaa gcctgatcga gtccctgaag atcgtgaaaa gccgcggggg
     2701 ctttgcctcc ataaactact tcgtgcatcc gggattcacc gactccgagg aggaatggtc
     2761 cgcccttcgc cggttcctgg acgaaacccg catcaatctc atccagatgc gcaacctcaa
     2821 tatcgatccc gaatggtacg agaaaagcct ggggctcccc gaagacgacc gtagcatggg
     2881 catgaggcgc ctcatcgacc gtctgagatc gtcctacccg gacgtccgtt tgggatattt
     2941 taatccgccg gtgaggggct agagaggatt cccggagacg aagcatgacg aaaccctttg
     3001 atattgcctt tgtgggggca gggattgtgg ggacggcctc ggccatgaag atggtccggg
     3061 ccaaccccgg cctgcgggtg gcggtaatcg agaaggagga cgctcccggg cgacaccaga
     3121 cggggcacaa cagcggcgtc atccactcgg ggctctacta tcgccccggt tctttgaagg
     3181 cgaggctgtg cgtcgaggga gcccggcaac tggtggagtt ttgccgagaa aagggggttc
     3241 ctttcgaact gtgcgggaag gtggtgctcg ccgtccggga ggacgagata atgcggctcc
     3301 acgacctgca cacccggggc agggccaacg gggtaccgga cctgcggatc ctgcatcccg
     3361 accaggtccg ggacatcgaa ccccatgccc ggagtctgct ggggctgcat gttccttcca
     3421 ccgggatcgt cgattttgga agagtgaccc aggcatacgt ccgggagttc ctgtccgggg
     3481 gagggtccct attcctgggc catagagtcg cggcagtcct cagggattcg tccctgattc
     3541 acctcgagac gggtcgcggg agggtatcgg cccgttccct ggtcaactgc gcagggctgt
     3601 attccgaccg ggtcgcccgg atggccgggc tgaatcctcc ctgtcaaatc gtgcccttcc
     3661 ggggggaata ctaccgaatt cgctccgacc ggagccgcct ggtccgaaac ctcatctacc
     3721 cggtgccgga cccccggttc ccgttcctgg gggtccactt cacccgaatg ctggacggaa
     3781 cggtggaagc cggtcccaac gcggtcctcg cctatgcacg ggaagcctac cgcaaggacc
     3841 gggtagtctg gtcggaactc ctggagacct tgagctaccc cggatttcgc cggatggcgg
     3901 gccgctactg gcgagccgga tggcacgaga ttctccgctc ctattcccgg actttgttcg
     3961 cccgggcact gcgaaacctg gtcccggaaa tccgcaggga agacctgttg cccgggggcg
     4021 caggagtccg ggcccaggca ctgggacccg acggggcact cctagacgat tttgtccttc
     4081 tcaaagaaga gcgcatggtg catgtgctca acgccccttc ccccgcagcc acttcctggc
     4141 cattgcggac attatcctgc aggaggcggc ccagatcctt tgaacccgaa accgccgcct
     4201 tccgtgcccc gacggggccg tcccgtcatg ctgttgctgt ggaaggaact gcatggccct
     4261 gccttgacgg ctgggctggg caaacggcag ggaccgcccc tagctctcgt cctcgtccat
     4321 gtcctccgtt tcgacttcct catgaacgga ctcatcggat ccgccatccg tgtgtgggac
     4381 tgcgggcggg cgggccatca cgttgctgcc tccgccgcgg gtgggaaacc ggggcgtgga
     4441 ggtaacctcc ggtggctcac cgccgtggga gggcgcggga agccgttctc tcctgcccga
     4501 gggtcccccg gggggtgggc tgccctgccc cgccgaagcg gtccccggcg gttgaggggt
     4561 ctggggcgca ggcggaacca cttttccttc ggcaaaaaga ctcaccacgt acgtctgccc
     4621 gtccttttcc agggaaacgc tccggggttc gatgcgaacg acctgatact cggccagctt
     4681 ctctccttcc cgaaccatga caaagggcga ttgggccttt ccggacttat ccttgccgga
     4741 aggctggcct ttcaatcgaa tgagggcctt cttgacgtca ccgcaaatga agacgccgtc
     4801 cagttggatg gacttgggct ggagccccgg ctggttggct tggggtgtgg gagcggcctg
     4861 ctccgaggga ggttttcggt cctgggcaaa gaggttcttc tccaccatga tgggagcggc
     4921 cccaaagcaa gtgcccgttt cgaatgcgaa ccacatcgcg agaaaacccg cgcaaagatt
     4981 cctcgagcct ttcatggaac tctccttccc gagggggccc cgtcgccgcc cccccggacc
     5041 gacccgttcc ccttccggaa atcctcgtcc gtcattgccc gagagcagga gcccggcata
     5101 tccgtccact tgggcgggag cagtcttgag ccccctatcc tccgccatga cgaggggttc
     5161 cgatgaaacg acccccttgg ggccaaaggt ctccggcccg cgcccggaat cctgcgaacc
     5221 atccccgagc cggcggatcg tccgctacta tatcgtctct tggtctccgt gaccagagga
     5281 caatggacgg ttccttcccg tcttccggcc cccgggggga aaaccagttt ctcttttccg
     5341 accgcccggg gaggagtccc ccgagtttcg gtctgcgtgg gatcgtttac tttcaaagat
     5401 caatttgtta agaatgctcc gaaagattta caggcctttt cgaggagcct ctatgagtca
     5461 agggtcactt ctggaagcct tccgcaccga actcaacctc caggaagatc aaacgaagca
     5521 gctccaatcc tcctattttg tggagggatt gccgctcatg gactccttcg aacagatcgc
     5581 cgtcacggac gaacatcggg tcttgcgggt gctggcccgg catctggacc tgcctctgct
     5641 ggaaaggcag gactacccgg aaaagcccgt cctgctcgag ggcgtttcca tgtttttcct
     5701 gcgcaagcat tccgttctcc ccattcacat ggacagtggc cgggttcggg tggtggtgaa
     5761 caaccccctg aacctcccca tgttgaacac cctcacccag tatttcctgg gcatggagct
     5821 ccagttgtgc ttggggctgc gggaagaaat ccgcacggcc atcgatcgtc tctacggaac
     5881 ggcggccaag gaagccgagg cgttgtcccg ttcggccgag ttgggttccc tgaacgggga
     5941 ttccttcgag gaagacctgg aacacctcaa agggttggcc caggaagcac ccatcgtaag
     6001 aatggtgaat gttttgatct cccgggccct ggacatgagg gcctcggaca tccatttcga
     6061 accgttcgac cggggcttcc aggtacgttg ccgggtagac ggggtgctct tcgacctgga
     6121 tcggcctccc aaaagcatgc aggcggccat catcagccgc ctgaagctca tggcaaacct
     6181 caacattgcc gaaagaaggc ttccccagga cggaaagatc aagctcaaat tcggcaaccg
     6241 ggaagtggat gtgcgggtct ccaccgtgcc caccattttt ggggaaagcc tcgttctcag
     6301 gctcctggca caggagtcgg tggactacag ccttgcctcg gtgggaatgg acccccagca
     6361 gttggggttt atcgaacaat tgatcgagcg gcccttcggg atgatcctgg ttacggggcc
     6421 cacgggctcg ggcaagacca cgacccttta cggagtcctg agaaagctca attccgtcac
     6481 ccgaaagatc atcaccgtcg aagatcccgt cgaataccag atcaacggga tcaaccagat
     6541 ccaggtgaaa ccccagatcg atctcacctt tgccaacgcc ttgcgttccc tggtccgcca
     6601 ggaccccgac gtgctgctca tcggggaaat ccgggacaag gagacggcgg acattgccat
     6661 tgaatcggcc ctcaccggcc acttggtctt gtcgaccctt cacaccaacg acgccccggg
     6721 ggccatcacg cgcttgcggg acctgggcac cgagtccttt ctcattgcgg attccctgct
     6781 ggccattctc gcccagcgcc tggtacgcat tctgtgccct cactgcaagg aaccttaccc
     6841 cgcaagggag gccgacatcc aacgcctgaa ggaggtcctc gaggaagtcc cgcacactac
     6901 gctctatcat aacaaaggct gcgaccggtg cggttttacg ggctttcggg gtcgaactgc
     6961 catcttcgag ctcctcccca tctccgagac cgtacggtcg gccatcgttg ggggaaagga
     7021 cgcgggcagc atcgctcaac tggggtaccg ggagcagtac cgccccctgt tgcaccatgg
     7081 gtatcgaaaa gccctcgacg gagtgaccac gctctccgag gtccttcgag tcaccagtct
     7141 tctgtcctgg accgggtaac ggaagaaacc cccaccaccg gaatcccatt gctgcggaaa
     7201 ggatgggcat ctaagacgat ggcatattac cagtaccatg cccgggacac catgggaggg
     7261 acgcatcggg gggtcatgga agccgcctcg gaggtcgagg cggcgggaaa gctcaagacc
     7321 gaaaagctct acccggtgaa gatcaagccc gtgcgcagtc agcgcccccg gcgggtgccc
     7381 gaagagcaca tcatccggtt cttttacgac ctttcggatc tattctacgc gggccttccc
     7441 gtggatcgcg ccctggccct catcagcacc aaccagaccc acaagacctt ccggcaggtg
     7501 gtccaggggc tcttggggga tgtccagggg ggaagcgatc tgtccggtgc catgggcaag
     7561 tatcgggacg tcttcggaaa tctttcggac cacatggtgc gggcggggga ggccggcggc
     7621 accctgggac cgatcctcaa gcgcctggcc gaatacctgg aacagcgccg cacctttcgc
     7681 cagagcatga tctcctccat gatctatccc ctcatactgc tgggagtgag tgcggtatcc
     7741 atggtcattc tactggtgta cgtcatcccg aagtttgccc ggatctttca cgatttgaat
     7801 caaaaggtcc ctttcctgac caatctgatg ctggaagcgg gcatgttcct caaggattac
     7861 ggttggatcc tgcccttggc cttcggtgcg gtctttttcg ggggaagggc cctgtatcga
     7921 aagcccaagg tccgaaagaa ggtggacaag atcctgtttc gaatgccgct cacccggtac
     7981 ctgatcctgc acagcgagtt gacccggttc tgcaggaccc tggggaccat gatcgaagcg
     8041 ggggtccccc tgctgcgggc tttgagcctg ggggaacaac tggtgatcaa ttccgtgctt
     8101 cgagaagcgc tggtgcccct gcaccgggag atcaaggccg gtcactccat gagcaacttc
     8161 ttccgttccc attccatgtt tcccgttcgt gtgggaacca tgttgcgcat tgccgaggag
     8221 caggggaacc tgggacaggg cctcatgggg ctgggagacc atttcgaaaa agagctccag
     8281 aggaccctcc agaggatcat ggccttgatg gaaccggcgg tgatcattgc cacgggcctc
     8341 atgatcggca ccatcgttct cagcatgttc accgccattt tcgggatcaa cgagatacag
     8401 ttttagccat ggcgccaagt cctttgaccc ggctgaaatc ccgggggttc accctggtgg
     8461 agctaatggt ggtgctggtc attttggcga tcaccgtcgg actggtcctg ccccgggtgg
     8521 gaggcggctg gaagcggatg gaggagcgcg aattcgtgca ggactttgtc cggactctgc
     8581 ggagcgcccg gttgcgggcc atgaacacgg gcacgatcgc catatttcgc attcggggcc
     8641 ccgagcggct ctacggtttc gaggttccgc cggaaacggc catccctctc aacgtggaca
     8701 tctacgccga ccggttggaa caggaccctt tcaccggcga tcacgtcgtt cttttctacc
     8761 ccgacggcag tctttccgga agcgacctgc agatcctctt tgaccagcag cgttccttcc
     8821 acattttcat tcaccctctc ttcggcacgg tccaggtcac cagaacggaa ccatgaaaca
     8881 gcccgcggca gccaaccctc ctgaatcggc gaaatcgcct tcccccggcc gcgaggatct
     8941 tcacctccgc cccgggagga cggcgcggac aaggcctgcc cgggggcgcc gcctgattgc
     9001 cggggccgat tctcgaaagg gatttaccct gctcgaggtc atggtcagcc tcaccatcgg
     9061 gctcatcctc ctgggtggcc tcatggggct gatttccgta tccctccact atgggcagcg
     9121 gttgaggcac aagagcgagg tccagccggt gctcgaggcg gcggcccaaa ccattctggc
     9181 gtttccggag aaggccgggg agggagccct ggtcatggaa tcgtttcccg acaaacctgc
     9241 ggtggaaatc cggctcgcac gggtattcga aaaggatgaa accaccccgc ccaccctggg
     9301 agaattgtac cgggtgcagc tcctgtaccg gggccagatg ctcgagttca gcatcctcgt
     9361 gcccactccc cccacccagt gagcggcgga ttctcgagga cctttactga tgcgacgcct
     9421 caggcaaaaa ccctcgaatt tcccggcaaa caaccgagga ttcaccttgg tggagctggt
     9481 tcttgccacc ttgatctcga ccctggtgat tggaatcctg tcggtgtccc tcgcgttttc
     9541 ccttcgtgtc tgggagcggc agcagaacca gaagcccaaa gatatcctca gtctcctgaa
     9601 cctgctcaaa tggcagctgg cccagttcga ccccgagccc atcgctctcg gttcggaaac
     9661 ggaaccgatt ttcaggggcg acgagacctc cctggccttt gccacggact attccaccaa
     9721 ggccatctgc aagggggtcc ccgccgtcgt tcgatacgta ttcgtccccg gagagcaggc
     9781 cgtttactat gcggaggtgc ctctgcatcc ctacgacccc gacgggatca agagattcct
     9841 ggaactaaga ccgcagaaac accgggggtg gccgccgttt cacgcggtgc cggtcgctca
     9901 gttttcccta gcctatattg caaaagacag tgtgagcgag caagccggtc cttgggaggg
     9961 ggaagagtcg gtccctgcgg gagtggtggt gtcctggaac gacggttcca acgatgtcgc
    10021 caccctccgg ttcgtggtgc ccaactctct ctttcaccga aagcctcaaa gctccttgca
    10081 accgacgggc cccgggagac ttcagggcac cggtcggagg aagcttttct gatgcggaac
    10141 caacgagggg tcgtcatcgt cgccgttctc tggatctgtt ctctgctcct gtggttcgct
    10201 ctccagatcg ggaccgaaac ccgcctcagg ggagaggagc aagtccacga aatccgcagg
    10261 tcccaggccc ttcacctggc cattggaggg gcctacgaag ccctggcacg catgggacaa
    10321 cctcttccca cgggccttga agacgctttt ggaaagagtg tccgagggaa aaacaccctc
    10381 cgacgggatt cggccgaaga gaactggcaa ccggacggaa cggggcacat cgtcgagtat
    10441 caaacggggg aggccctggt tttcattgag cctgaaggtc ggaaggtgaa cgtcaaccgg
    10501 gcgaaccggg aacagctcaa aatggtcttc gagcggaccg gcctggccga agacaggatc
    10561 gaacaactgg cggacgtgac cgcagatttt ctggacaacg acgacctgcc gcgcctccac
    10621 ggcggagaaa aggactacta ccagcgcctg gggcttccgt atccgccctt caacggtcct
    10681 ctgctgagcc tggatcaaat gttgctcatt cccgggatta cccaggacct cttcttcgga
    10741 tacggtccga gggaaaacga agaccggaac gaggcctccc cgccccccgt tcccggcttg
    10801 ccaggaaata attccctatt tcgaatgttt acggtttatg gtaccaatac gaccttgccg
    10861 tccggtgagc tcgaggaaga gatccaagag aagatcgaga cttggcgacc gggagaaatc
    10921 taccgcttac tttcctgcgg caaggcttat acgggtcctc ccgtggtggt gctttggctc
    10981 attgtgcgtt tccagcccca gctcccgggc gggtacgaag tgctctatcg gaagattttg
    11041 tgagggtcga tccaccggga atcaacgaga atgctgagag gtcttgtcaa gcggcgcccc
    11101 aggcaattgg tggcggtcta tgtggaaccg gagcgcatcg aagtcttgcg tgcccgtcga
    11161 cactggcgct cgtggcgcat cgaatccacg gaacaattcc cggtaccccg gggagagggg
    11221 ctctatgact tcctccagag gctcgctctg aggccccggg acaaaaagga aacggcgctg
    11281 gtcctcttca ttccccggac tttttacaat ctccatcgag agcattaccc ctccaccctc
    11341 cgggaccagc tgcaggaagc catcgccttc gactggcagg aaaacgtctt tgtcgaggag
    11401 gaacagtccc ttcatttctg cggccccccc gtaggggtgg accaccagat ctccgttccc
    11461 atcttctcca tgcagcgcga tctctacgac aagctctacc aggccctggg cgccgccctt
    11521 ttcgacgtct tcgtcgtcat cccctcggcc ttggtctaca aagctctcct cccctccatt
    11581 gtgccccgag acgaggagga atcgtccctg gagatcgtgg gaaggttcct cgagccccat
    11641 cgactggaaa tccaccgcta tctcaagggt gaattcctgg actccttcct cgtcagccga
    11701 aacctggaca ccctgcgcct ctttcgggaa gccctggcat gcattggaaa cggccagtgt
    11761 ccgccggagc ggtccatcca cctgttgtgt tccgacggcg agcgcgccga ggactacgtc
    11821 cttgcttgga aagacgagaa tctgcccatg cagatccaca agtccccgga accgctgctc
    11881 ctccattggg tgaactacct cctccaacag gacaaggtcc aaaccttcaa cgcccccctg
    11941 ctgctcaaac cctggaaagt gcccaaagtg gtctggccca tgctggccct ggcggccatg
    12001 tttgccgcct ttgccgccta ccaggtccat tcgttgggac agatgcagca gagttccaag
    12061 cgtatcaaga ggcaaattgc ccagctcgaa acccaatgga aacccgtgga gcagctccag
    12121 acccgcgtgg gcaagttcga agaagaccgg aaaaccctca acgaattcaa ccaggaaggc
    12181 tatcccctcc tggaaatgct gtccctcctc acccagatca cccccgagga tacctggctc
    12241 aactactttt ccattcgaaa gggccagatg gtgttgcggg gagaaagcaa gtccgccatc
    12301 aagtaccttt cggagctctc caaggtggag gggttcgggg atgttcggtt ttcatccccc
    12361 gtgacccgaa atcccagctc cgacttggaa cggttcaacg tccaggtaca gatcgatgtg
    12421 gagaagctca agaaaacgac ggattcgttg ggcatccaaa ccctcgacac cccggcggcc
    12481 gacgccgggg gagaatccgt ggcggaaagc cccgagcccg caacggcaca aatcccgccc
    12541 ctgagagtcc cggagaagga acaggacgag gaggaaacgg aggacgcgga ggaagccgag
    12601 gaagctgaag taacgacggc ggacacatcg gaatcggaag aggaagatcc cgaagaagag
    12661 cagggccgtt gacccgaggg ggggtctcga tgaaatatcc gaaaatttcc tggagcgaaa
    12721 ttcagaaata ccgcctggca agcctgggaa tcgcggccac gatccttgcc ctggtcgtgg
    12781 tccatgtgtt ctggctggca cccgtccttt ccgagaagga aatcctggaa aaccgtctga
    12841 aacagcaaca agacctcatg caaaagtacg aggacaagct caaacaggcc cattccatcc
    12901 gggaaaacct ggtcaagaag gaagccgagt tgcgggagat gcagaagcgg ctgtttcgag
    12961 gcaacgatcc ttaccagctg gcggcatcgc tgggggagac cttctctccc aagggagggg
    13021 ggaaggccaa gctggaaatc aagacctacc aggtcattgc cagtaaggaa tacggcttgt
    13081 atcaggaggt gcacctgcgg ttcaacttga tgaccaccgt ggaggggctc catttcttcc
    13141 tggatcgcgt gggcaaattt cagacggcca tcctggtcaa ggaaatcaac atccagaaga
    13201 ttcagaagaa gggaggtccc gacctggtgg tgaacgtgat cctggcggcc ctgatggaga
    13261 agggagaaaa gtcttaaaat cccgtgccgt ttggtctata cttctccacc cgcaaagtga
    13321 cggcgcgctt tcgtccctac tgggacctcc tcctcgggtt cggaacggaa tcttcttcga
    13381 gtcctgtggc gactcgacaa acgatttgtc atgaatgagg cttacgcatg aaatggtacc
    13441 gggccttcct ttcgctcctc ctcgccgcca tgctggccgc ctgcgcccag ccttccccac
    13501 tgggacgcgg acaggcggat acgggaattc gagcccctcg gcctcccgcc tccgccgccc
    13561 ggatgccgcg ggagatttcc acggatttgt ccaagtccgc cgacgcgacg tcggccgacg
    13621 cggcaaagaa gaagcccgtc ctgccgtcgg tgaccgccaa gccttcctac gggctcatga
    13681 gtcctccctc ggatcagaaa cctgaaaagc ttcccctgtc ggacaaggag aaacagaagg
    13741 tcgttctgaa cttcgaaaag gcggacgtgg ccgaggttac caaccagatt ttcggcgatt
    13801 atctcaagct caattatgtt ctggatccgg gcctccaggg acgcctcagc ttctatctcg
    13861 agggggaatt caccaaggaa gagctctacc agatgatcct caaggcctac gaggccaaca
    13921 acatctccat tgcaagccgc aagggcatct tttacatcca gcccctccaa aaggggtcca
    13981 gcagcagtct gccgctggcc accaaggcgt tgctccagga tgaaaaggcg ggggcaagac
    14041 cggtcattgt cctctaccgt ttgcagttca tggaagccaa gcaggccctc aacgtcatca
    14101 agaccttcct cactccgggc aggcccgtcg cttccgacaa cctgaccaac agcctcatct
    14161 tcgtggagga cgccgaaaac gcccgaagca tcctggacgt gctcaaggcc ctggatatca
    14221 acatcctccg ggaggtcagc atggaaatcg tccccgtcca ggggatgtct cccatggatg
    14281 cggcccaggg catggaggcc ctggtcagta aaatgagtct cttcaaggaa agcaccgtga
    14341 agagcaacgt tgccttcatt cctttgccca atttcggcgg ggtcctggtc ctggcccaga
    14401 atcccgaagt tttgagaatc gccaagtcct ggctgacggc cctggacgtc cagggcaagg
    14461 aagccgggga acaggtctat gtgtacttcg tccaaaacgg gctggccgga gacatcgccg
    14521 acattctgaa ccaggtcttc ggcctgggcg gcaccaccgg cggccgccgg ctgcagcagc
    14581 aggtggtccg ctcctctcgg cagttcggag gcacggggca ggggctggga ggaacgggcg
    14641 gattctcggg gcggaccttt ggaggcactt ccaccgggac gggaggcttt gggagctcgg
    14701 gaggcttttc ttccaccggc cgaacctccg ggttctcctc cgccgcccag tccggagacg
    14761 gctccggtac ctcgacggga acgagcggca cctcctcttt cggaaccggt gccggggcgg
    14821 cggcaagaag ggcccgggtc cccggagggc tgggagcccc ggggcaaacc ctccccaata
    14881 tccttagcgg cgaggcggtc atcattcccg acgaagtcaa caacgccatc gtcattcgag
    14941 ccaacgccaa cgactatgcc aagatcagga agaccatcga gaccctcgac attctccccc
    15001 gggccgtcct catcgaggtg atgattgccg aggtcaccct caacaaggag ctccagtacg
    15061 ggctccaatg gttcttccag gacatcggca tcaagatcgg gggggaggag gggaagttca
    15121 ccatcgccaa ggtcaatccc ggggccggca cctcgggaac ggggaccgac gggaccggcg
    15181 gatccaacgt gaacccggac aataccaacc gcaaccggag gaccttcctg aatctggccc
    15241 ccagcgcaac gggaggtctg tccaccttct ggggcaccct gcctcacaac ggggttcggg
    15301 atgtggccgt tctccttgac ctcctgggtt ccaagaccac cgtcaacgtc ctgtccacgc
    15361 cgacccttct ggccacggac aatcagcagg catccatcac cgtgggcgga cgggaaccca
    15421 ttcagaccgg ccagaccctc ctcgagggcg gggtttccac caacaacatt ttcaccacca
    15481 ttcagtatga agaaacgggg atcatcctga acgtgacccc ccacatcaat gcggggggac
    15541 tcgtgcggct ggaagtggaa cagaccattc ggaacgtggc cgaacagacc acccagggga
    15601 tcaattctcc ccgttttacc gagagaaacg tcaagaccac cttgcttgcc caggacggct
    15661 ccaccgtggt cataggcggc attatcgagg atcgccggtc cgacgggaaa acggggatcc
    15721 cggtgcttca ggacatgccc atcatctggc ccctcttttc cacgacggac aagaaaatga
    15781 accgcacgga gctcatcatc gccatcacgc cccacgtggt ggaccatcgc gagagcgatg
    15841 tgacccgcga gtttctcggc aagttgaagc agctgaagaa acgcatcgaa aactaggtgg
    15901 ggctgcgcag gaatcgggag tttggcgcat ggtgtgagag gggctctgat acgaagctgc
    15961 attcaagccg aaactgccca tccgcaggac tcagcccttt gctcgaaatc ttcactcagc
    16021 atgt
//
LOCUS       DAOOVC010000090        15866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2482_length_15866_cov_6.611410, whole genome shotgun sequence.
ACCESSION   DAOOVC010000090 DAOOVC010000000
VERSION     DAOOVC010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15866
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2482_length_15866_cov_6.611410"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(960..1427)
                     /locus_tag="PLM79_11700"
     CDS             complement(960..1427)
                     /locus_tag="PLM79_11700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOV87020.1"
                     /translation="MSETGFVLKVESHPYFQRFKPEHLAILEKCASMAEFEPGSHLFH
                     EKERATRQYLIVEGRVALDLHVPGRDPWTLMTAGEGGIVGYAWAYPPHRYYYTCRAVD
                     KTRAIVLDAEYLAKKCKEDTDFGYEVMVGCTSIMAERLQAAELQLLNLVLKCY"
     gene            complement(1662..2672)
                     /locus_tag="PLM79_11705"
     CDS             complement(1662..2672)
                     /locus_tag="PLM79_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOV87021.1"
                     /translation="MHDPWLIVVMIVLVALVFDYTNGAHDSANAIATVVSTKVLSPGT
                     AVVMAAVLNFVGALVGTSVAHTVGGGIVHPGMVTGSQTLVLSALLGAIFWNALTWYLG
                     LPSSSSHGLIGGLMGAAVAHGGWQTLNYHSIFMKVLVPLILSPMAGLTGGYLLMLGLA
                     WLCFRANPGKANKTFRRLQVLSSAFMATSHGLNDAQKTMGIITLALFMFHWIPTMEVP
                     LWVKLASALVMGLGTATGGWRIVKTMGHKIFKLEAIHGFAAETSAAMVIALASSVGAP
                     ISTTHTISSSVLGVGASKRLSAVRWGVAGNMVVAWVLTIPMAALVSGGIQLFFCWVGF
                     ND"
     gene            complement(2665..3303)
                     /locus_tag="PLM79_11710"
     CDS             complement(2665..3303)
                     /locus_tag="PLM79_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOV87022.1"
                     /translation="MAFSLFPRSAKFFDLFIRQSQKLVQAATILNDIFQNYSNVEEKC
                     AQIHILEEEADLISREIARQLSLTFITPIDREDIHHINIAQEEIQNLIDAISTRIGLY
                     GFSNIRTTAKNLISTIMLMINETDKMVLDLSRKRNVEDKYNYVKQLKNDCETLLSMAL
                     AEMYDSRLSIPEECIDVIDMIKWTQIYDRIEECVYRTGRLANIIEGITLKNA"
     gene            complement(3650..4807)
                     /locus_tag="PLM79_11715"
     CDS             complement(3650..4807)
                     /locus_tag="PLM79_11715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV87023.1"
                     /translation="MIEAAPKKGRILSLSLAHLMNDWYMNYIQTLLPFLIAGGMAVSK
                     GAFLVSAFTITSSLLQPVAGYLVDQRNQRWLVYVGTLWMGVLLSLVGVLSSYPLMVFL
                     VTLAGLGTAAFHPQASAMVTAVSGRKKGFFQAVFITFGNIGWALTPLVVVPFTQEYGM
                     ESTPYFALPGVLVALLLWGTAPRLSAGSRRPSPPPLWDTLRRAGWELVKVVSVVALRS
                     LSYFSLVAFLPLYLQHRDVSLVHGGRLLFLMLFMGAMGGLGGGFLSDRFGRKPVIVGS
                     LVLSTPLFFLFLRTDGPVSWLFLALGGAALLASFSVTVVVAQEIISRNAAMAAGLMLG
                     FGIGIGGLGVGLMGLLVERIGIDKAIGLLISLPLAAGLLGLSLGKRKGVEE"
     gene            complement(5315..6917)
                     /locus_tag="PLM79_11720"
                     /pseudo
     CDS             complement(5315..6917)
                     /locus_tag="PLM79_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810975.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            7172..7756
                     /locus_tag="PLM79_11725"
     CDS             7172..7756
                     /locus_tag="PLM79_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV87024.1"
                     /translation="MNLKEKIILEACRLFSLKGYMSTSIQDVMAASGASKGGLYNHFK
                     SKEELFRAVLDHARTVWREKNLVGLDRIDSPMGKLIRLVENYRDNYLLDAESFPGGCF
                     FINLSVDLDDQDPALAVELERGFTGLRGMILRLLEESKRMGELKEGVYVNDVGEVVFA
                     SIMGAAVAFGQEKSRVRLNRTITAILNYLKGLLR"
     gene            7857..8993
                     /locus_tag="PLM79_11730"
     CDS             7857..8993
                     /locus_tag="PLM79_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOV87025.1"
                     /translation="MIDFTFTEEQDMFRKAAREFAETRVAPFVPEMEKTGKVPPDLVK
                     ALGEAEMMAITIPEQYGGLGLGFVARMIALEEISRISVATGMMLQVLALGIDPIVRFG
                     NEAQKKKYLPGLARGERLATVAVTEPTGGSDPTGGRTVYSKDGTDYVITGRKCFITNS
                     HIADVVTLLAKDENDPKAFSAFIVEKGTPGFKATHEEHKVGMKGCNTGELSLEKCRVP
                     AENLVGREGKGLRVTMSAIGDVGRGGMVGCALGLHTACLEASLKFAKDRILYGKPISV
                     LQTIQNKLAEMKIDLEAGRLLAYRAAAMQDRGEPSSNEFAVAKYFTTETAQKAAKMAV
                     DIHGGYGCMEEYAVSRYLRDAFVLGPSAGTSDIMKVIIARWVLA"
     gene            9059..9853
                     /locus_tag="PLM79_11735"
     CDS             9059..9853
                     /locus_tag="PLM79_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV87026.1"
                     /translation="MKIVVCVKAVPDPKEICRIRIDPVSKTLTRCEVPLVVNLLDKHA
                     LEAALAIKESSGAFVTVLSMGPPPADRIVKECMSLGADRGILLSDRAFAGADAFATAH
                     TLARGIEKAGPFDVIFCGMASSDGATEWVGPQIAAFLDLPVVTMVRKFESRQGDRWLV
                     LADWQNGYRRVEVRLPALFTVTRELNRPRALSFSGILKARSKTIEHWDLSSLGVSADS
                     VGAQGSPTLVTEISPVENRRDAEMLRGTLEEKADQFVSRLAELGIV"
     gene            9867..10889
                     /locus_tag="PLM79_11740"
     CDS             9867..10889
                     /locus_tag="PLM79_11740"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOV87027.1"
                     /translation="MKKRLGPVWVMAEQRGCEVETVSLQLLGKARRLADELGVACESV
                     ILGHNLGEAARRLIAGGADRVFVGDAPHLALYQSLPYRDILCALVEEHKPEILLLGST
                     FLGRELAPLAAARLKTGLAAHCTGLRHDKDGNLEQQIPAYGGLMSIVCPNRRPQMATV
                     AKGVFKAAEPDENRKGEIVPVRVPGETELGVRTLEIVQEAPPEIELEEADVVVCGGAG
                     AGSAEGWEIVRELAVALGAALGCTRPVVDEGWAPLDAMIGQSGKMVSPRVYIGVGLSG
                     EQQHMVGVVGAQFMAAVNQDPKSAIFQQVDLGVVEDCRKFLPVLIEKIKAFRKERTFC
                     RPPSVS"
     gene            11041..12342
                     /locus_tag="PLM79_11745"
     CDS             11041..12342
                     /locus_tag="PLM79_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV87028.1"
                     /translation="MGDMKKLAELVKELQGRLAVCMRCGLCRSVCPIFAQTGREPDVA
                     RGKLALLDGIIEGVFRGPRGVNDQLRRCLLCGACGANCPSGVKTLELFLKARALLAGY
                     LKLSPLKRLLFRALLPHPKVFDRLLEWGSGLQGIFLKPVDDFLGTSCGRVPSPLSGRR
                     RLKAPALVPFHRRVSSLDRPAGKSGLRVGFFVGCLIDKVFPNVAEAILEALEKHGVGV
                     FLPGDLGCCGIPALSAGDATAFGRLVRHNLEKLTSEPFDFLVTGCATCTATIRNLWPV
                     LYPDIEDGKRAQVRALAAKTLDISQFLADKAGPLHGTGPTSGDAVPLAYHDPCHLAKS
                     LGVTAQPRALLRANGLYRFEEMAEADECCGCGGSFNLEHYDLSASIGRRKRENIARSG
                     CKTVATGCPACMLQLSDMLSQAGDRIRVKHAVEIYAEVLRP"
     gene            complement(12369..13760)
                     /locus_tag="PLM79_11750"
     CDS             complement(12369..13760)
                     /locus_tag="PLM79_11750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV87029.1"
                     /translation="MTFEELHLTPPILKALSRCKHHHPTPVQAEAIPKVLTGRDLVAS
                     AQTGTGKTAAFVLPALQRLTTNPLPSKTGVRILVLTPTRELATQITEAARAYGRYTNV
                     HSVAIYGGMPFGDQIRALAQIPDMIVATPGRLIDHIWRGRILLSQVELLVLDEADRML
                     ELGFIDDVEFISDAVPEECQRVLFAATMGDATARLAQKFMNDPDRVRIAPGKITHDRI
                     EQRLHMADNLVHKNRLLGHLCKDPSLTRAIIFSATKRDADSLARQLNQQGYASAALHA
                     DMSQAARNRTMANMRRGKIRLLVATDVAARGLDITGISHVINFDLPRSPEDYVNRIGR
                     TGRAGESGTAISFASRADLPYLDRIEHYLGHNLSVHVVPGLEPEHPVHRHNGSGKGKA
                     RPAGSNVPGKQGRHRPRSAGGQGIGGGGSQAGVGRLARERRNGKAAGESANGKPRRTP
                     HHGDRVTAGKASL"
     gene            complement(14189..14521)
                     /locus_tag="PLM79_11755"
     CDS             complement(14189..14521)
                     /locus_tag="PLM79_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOV87030.1"
                     /translation="MNIYVGNLSFKTTERELQEAFAVFGEVGSVRIITDGYTGKSKGF
                     GFVEMPNTAEAKAAMEGLNGKELGGSVLRVNEAKPREDRRGGGRREGGHFGGRGRGGG
                     GGGGGRRF"
     gene            15617..15853
                     /locus_tag="PLM79_11760"
     CDS             15617..15853
                     /locus_tag="PLM79_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87031.1"
                     /translation="MHSRAFVVAGAMKDDLLSDFQAAVLMGIEKGITLNEFRKDFDSI
                     VKRHGWSYKGGRGWRSSVIFKPKFVKNFYLTAPK"
BASE COUNT         3204 a         4777 c         4798 g         3087 t
ORIGIN      
        1 attcgtaatc agcaggtcgc gggttcgaat cccatcgccg gctccaattc aatccataac
       61 cgggccggga tccacttttc gccgttctcc atcctccccg attgatccct gccctgtccg
      121 cacttgtggg gatttcgcgc tccgccacat cgccttggca actgctccca acatagccgg
      181 atcgcgaaac gacgagagac caaagcactg gagtgtcacg gaatacatat gtgtcacaga
      241 cttggaccga cactcgtggc ggcatctccc acaagcaccg caggcaagac gatccccttg
      301 ccgtggtgcc tttggcgtct gaagcggggt ttgccgcagg aatgccggct atcccacacc
      361 ctttccacgg gtcccggcga ggggacttcc gcgccggact cctgcttcga gtcgcctgcg
      421 cggaagcgat ccccggggtg gggatgcggg atgaggcctt ccggccagaa agggatcgac
      481 agaccggcag cgggaaattc ctgaccatcc ttgacgattg aatggaagag ggaatttccc
      541 gggcttgccg tggggtcgga tcaagccgat tcacccgcaa ggacttgcag ccgttgaggt
      601 tgctgttgat cgagagtgaa gacggattgg atcccgggtt tgtgggaagg atgagcaaag
      661 cggccgctca agacccactc caacgatttt gcgggcccgc tgccttctgt gagaacgaga
      721 aaaaggcccc tcgattctcc gggaaaggct gctgaagaga atcgaggggc acaaaaagga
      781 aaggatacga gggaaagagg gaccctccga aggatcgtct tccctcccgc aatgttgccc
      841 ggggacggcc ttggggagag cttcttacaa ggcccgggtc gatgacggca aattcagcgg
      901 ggggctcccc cgttcacaaa gcggtgcagg gtccacggac cttctcccgg cggcattcgt
      961 tagtaacact tgagcaccag gttgagcaac tggagctcgg cagcctgaag gcgctcggcc
     1021 atgatgctgg tgcatccgac catgacttcg tagccaaaat ccgtatcttc tttgcacttc
     1081 ttcgcaaggt attccgcatc caaaacaatc gcccgggtct tatccacggc gcggcaggtg
     1141 taataatagc gatgaggggg ataggcccac gcatatccca caattccgcc ctcgccggcg
     1201 gtcatgagcg tccaggggtc tcttcccggg acatgcaggt ccagagcaac tcgaccttcc
     1261 acgatcagat actgacgggt agccctttcc ttttcgtgga acaggtggct ccccggttca
     1321 aattcagcca tcgaggcgca tttctcaagg attgccaagt gctcgggttt gaatctttgg
     1381 aaataaggat gggactccac tttcaacacg aatcctgtct cactcatatt cgacctccat
     1441 tggtgacgat gaattccaag atggacttgg agaagggaca ttcgatgata aaagagccgc
     1501 tttccccgaa taacacccgt catggttagg tgtcaacaaa catttgctcc cacggaacgg
     1561 gcatgaacgg gacgtcccac gggggtggat cccggccgca tgccggaggt ccgaggacat
     1621 cggcccccac gcggagaagc ccgtgggcat tggcggcccc tctagtcgtt gaaaccgacc
     1681 cagcaaaaga acagttgaat cccccccgat accagcgccg ccatggggat ggtcagcacc
     1741 caggcaacca ccatgtttcc ggccacaccc cagcgcacgg ccgaaagccg cttggaggca
     1801 cccaccccca ggaccgaaga ggaaatcgtg tgggtcgtgc tgatgggagc ccccacgctc
     1861 gaggccaatg caatcaccat cgccgcagag gtctcggcgg caaatccgtg gatggcttcc
     1921 aacttgaaga tcttgtgccc catggttttc acgattctcc atcctcccgt ggccgttccc
     1981 agacccatca ccagggctga ggccaacttg acccacaggg ggacttccat ggtgggaatc
     2041 cagtgaaaca tgaacagggc aagggtgatg atccccatgg ttttttgggc gtcgttgagg
     2101 ccgtgactgg tggccatgaa ggccgacgac aggacctgca gcctacggaa ggtcttgttg
     2161 gcttttcccg ggttggccct gaagcagagc cacgcaagac ccagcatgag gagatagcct
     2221 cccgtgagac ccgccattgg cgagaggatc aagggcacga ggaccttcat gaaaatcgag
     2281 tggtaattca gggtctgcca tccgccgtgg gcgaccgccg cacccatcaa tcccccaatg
     2341 aggccgtggg aagaggaaga aggcaggccc aggtaccagg tgagggcgtt ccagaaaatg
     2401 gctcccagca gggccgagag caccagggtc tgagacccgg tgaccatccc ggggtgaacg
     2461 atcccgcccc cgacggtgtg ggcaacgctt gttcccacca gggcccccac gaagttcaaa
     2521 acggcggcca tcaccactgc agtcccgggc gaaagcactt tggtggatac gaccgtggca
     2581 atggcgttgg cactgtcatg agcgccgttc gtgtaatcga acaccaacgc caccagcacg
     2641 atcatgacca caatcaacca aggatcatgc attcttgagc gtaatcccct ctatgatatt
     2701 ggcgagccgg ccggttcgat acacgcattc ttcgattctg tcatagatct gggtccactt
     2761 gatcatatcg atgacatcga tgcattcttc gggaatagaa agccgggaat catacatctc
     2821 ggccagggcc atggacaaca aggtctcgca gtcattcttc aactgcttga catagttgta
     2881 cttatcctcg acattcctct tccggctcaa atcgaggacc attttgtccg tttcgttgat
     2941 catcaacatg atcgtagaaa tcaggttttt tgccgtcgtt cgaatgttgg aaaaaccata
     3001 caacccgatt cttgtggata tggcatcgat gaggttttga atttcttcct gagcgatgtt
     3061 gatgtgatga atgtcttccc ggtcgatggg ggtgatgaag gtgagagaaa gctgccgggc
     3121 gatttcacgg gagatgaggt ctgcctcttc ttccaggatg tggatctgag cacacttctc
     3181 ttccacgttc gagtagtttt ggaagatatc gttcaagatg gtggcggctt ggaccagttt
     3241 ttgactttgc ctgatgaaaa gatcaaaaaa cttggctgac ctgggaaaca agctgaaggc
     3301 catcttgcca cactccctat ccgaatacct gcagcaaaag atcccttacg acgagatacc
     3361 tttttaaagc atgtatgtat attttgccac cttcaagtgc gattcggcac gaaaaggcag
     3421 gcggaatgag cccggaagcc tcccaagaac cccttcaccc ctggaggcag cgcccttgaa
     3481 tcgtctcacg gcgtcgtttc agcccgggtg cgaatccgga cttcacgaca ttctccgaac
     3541 ccccgcttct ctgggaagag agccccgccg tatccccggt tggcggtggg ctgccgcttc
     3601 atggggttca cccggcggac ttcaaggcaa ccgtgtcggc ccctcgggat cattcctcga
     3661 ccccttttct cttccccaga ctaaggccca ggagtcccgc cgccaggggt agggagatga
     3721 gcagtccgat agccttgtcg atgccgatcc gttccaccag caggcccatg agaccgactc
     3781 ccagtccgcc gattccgatt ccaaacccca gcatgagccc cgccgccatg gcggcgttcc
     3841 tggagatgat ctcctgggct accaccacgg tcaccgagaa agaggccagg agagccgcac
     3901 ctccgagggc caggaacagc caactcaccg gtccgtccgt ccggaggaaa agaaaaaaca
     3961 ggggggtcga gagaaccagg gaacccacga tgacgggttt tcttccgaat cggtccgaca
     4021 ggaatccgcc cccaagcccc cccatggctc ccataaagag catgaggaaa aggagccgac
     4081 ctccgtgcac gagcgacacg tccctgtgct ggaggtagag cggcaggaac gcgaccaggc
     4141 tgaaatagga cagggaacgc agggccacca ccgacacgac cttgacgagc tcccacccgg
     4201 cgcgtcgcag cgtatcccac aggggcggcg gagaaggtct ccggctcccg gccgaaagcc
     4261 ggggcgccgt gccccagagc agcagagcca ccaggacgcc gggcagggca aagtacgggg
     4321 tggactccat gccgtactcc tgggtgaacg ggaccacaac gaggggcgtc agcgcccacc
     4381 cgatattgcc gaaggtgatg aagaccgcct ggaagaagcc cttcttccga ccgctcaccg
     4441 ccgtcaccat ggctgaggcc tgaggatgaa aggccgccgt ccccagcccc gccaaggtca
     4501 cgaggaagac catgagggga tagctgctca aaacaccgac caggctcagg aggaccccca
     4561 tccagagggt gcccacgtag accagccacc tctggttcct ctgatcgacc aggtagccgg
     4621 cgaccggctg gagaagagaa gaggtgatgg tgaaagccga aaccagaaaa gcccctttgc
     4681 tgaccgccat gccccctgcg atcagaaagg gcagaagggt ctgaatgtag ttcatgtacc
     4741 agtcgttcat cagatgagca agggagaggg aaaggatcct tcccttcttg ggtgctgcct
     4801 cgatcatggg attccctgtt aggccgttat ggtgtggacg gttcaactta gggccgggga
     4861 gttcgcgccc ggctactttg cacaacctgt gccgtatccg tcaagcatct tgacttggga
     4921 tccgcggcac cgggcagatc cccggcgagg cggtctctcg aacgaggcac ccaggccccg
     4981 acttcctccc ggtttggccc gggccccgtc cgtccgctcc aagggggacc ccgcagaagc
     5041 cccttccgct ttcccttcca ggactcgaga ctgtacaggt ttacacaaat gcccgacaga
     5101 atacgtcccg gaccaacggg acggggaaat catgtgaaag gatggccttc ccaaggaaaa
     5161 gggaggacac caatgacggc tgcaggttcg aaggagtcct cctcgtggga agaggacccc
     5221 ttgggactgg aacccgggaa gggggacgga caatgggcat acgcaccccg agggcgtgcg
     5281 ggccgtccag tcaaggaggg gggaagaaag cctctcagct ccccgctgcg accttttcca
     5341 accggatggc gcaaaccttg tactcggggg tcttgctgat ggggtccatg gccgggctgg
     5401 tgagcaggtt ggcggcagct tcctcgaagt ggaggggcat gaacaccatg ctttcctgga
     5461 cccgttcggt gatgcgaacc ctggacaaga gagaacctcg gcgactggtc acccgcacat
     5521 agtccccggt tgcgatgtcc aaccccctgc cgtcctcggg atgaaggtcc gtgatcaact
     5581 cgggcatttc cttttccagg tgcgcacgtc tccgggtcat ggtccccgtg agataatggg
     5641 aatattccgt ccccgtggtc atccagaaag ggaactcttc gttggggact tccgtgggaa
     5701 tccggatcgc tcaccacggg gttctctccc aggatgacca tcgcctggat cgttccatcc
     5761 acgcagccct gtatcacctc caggatggtg agccccacct gggagggaag cttggcatgc
     5821 cacgcggcct cgaatttctt ccgattgtcc ggaaccgtta cgggctggta tcccggaaat
     5881 acattgggca gtccccccat gtcgcaagct ccctggacat tgttctgacc ccggaaggga
     5941 ttgaccccgg tggacggcct cccgatctgc ccgcacagca tggcaaggtt cgccagaccc
     6001 ttgacgttgt ccaccgccgt ccagtgttgg gtgatcccca gacaatagac gacggcgccg
     6061 gccttggacc gggcatagag acccgccacc tcgacgatgg tggacacatc cacccccgtg
     6121 atatccgaca cccgctcggg aggatattct tcgatcctct tcagaaaagc ctccacgttt
     6181 tccgtgcgtt catccaggaa ggcctggtta tgccagccgt tcttgaggat gacgtgcatc
     6241 atcccgttta aaagggccac gtcccttccc aggcggtgtt gaacgtggat gtcggcaatt
     6301 cgggagatgt gggttttccg gggatccacc acgatgagct ttcccccgtt ggccttgcac
     6361 cgcatgagcc tcgtggaggc gatgggatgg gccgcggtgg tgttggaacc aatggcaaag
     6421 atgcagtcgg cttcttccag ttcctcgaag gagttggtcg tggcgccgct gccgaaggcg
     6481 agggcaagac ctgccaccgc gggaccgtgt cggaggcggg cgcggtgatc cacgttgttg
     6541 gttccgatgg cggtgcggac gaacttctgg aacaggtagt tttcctcgtt ggtgcatcgg
     6601 gcggaggtca agcagccgat gctttcggga ccgtaggcgt ctcgaatcct gcaaagctcc
     6661 cctgcagcat gctccatggc cttctcccag ccgcattccg cctggacgtt cccctttctc
     6721 aggaggggtt tcccgagcct gtcggggtgt tacacgaatt cgtggacgtt ccatcccttg
     6781 atgcacagtt tcccctgatt ggtcgggctg gttttggatg gcagggtgca cacgatccgg
     6841 ccgtccagga cttccagcac gagaccgcat ccgcaggcgc aatagggaca cgtggtgacg
     6901 atcgacctgt aatccatccg gttttcccct ctcgatgaaa aatcggttga ctgattcccg
     6961 acgatccccg cctccgggtc ggcaggagcg ttatgggcca tggggaataa atcactcgga
     7021 atgtaaagac caagcatggg tcccgggcaa gtctccttac atccatgaga ggagcccggg
     7081 gatcgccttt ccccttgacg ggacccgctg ccttccgcta aaaataggac cgaccggtcc
     7141 gactctttcg ggggcactgc ccaaaaaaag catgaatctc aaagaaaaaa tcatcctcga
     7201 agcctgcagg ctcttttccc tcaagggcta catgagcacc tccatccagg acgtcatggc
     7261 ggcctcgggg gcatccaaag gcggccttta caatcatttc aagagcaagg aagaactctt
     7321 ccgggcggtt ctcgaccatg ccaggaccgt ctggcgtgag aagaacctgg tggggttgga
     7381 ccgcatcgac agccccatgg ggaaactcat ccgcctggtc gagaattacc gggacaacta
     7441 tctcctggat gccgagagct ttcccggcgg atgctttttc atcaatctgt ccgtggacct
     7501 ggacgaccag gaccccgctc tggccgtgga actcgaacgg ggttttaccg gcctccgagg
     7561 gatgatccta cgactcctgg aagaatccaa acgaatgggg gaactcaagg aaggcgtcta
     7621 tgtgaacgat gtgggggagg tcgtcttcgc aagcattatg ggtgcggccg tcgccttcgg
     7681 gcaggagaaa tctcgggtcc ggttgaaccg aaccattacg gccatcctca attacctcaa
     7741 gggactcctt cgttagagga tcgtccccgg aacgagaaat tttcgcgaat tgcagaccga
     7801 cgggtcggta gggaaggctc aggaaattcc accccaacca tgaggaggag agtccgatga
     7861 tcgatttcac cttcaccgaa gaacaggaca tgttccgcaa ggcggcccgg gaatttgccg
     7921 agacccgtgt ggctcccttc gtgccggaaa tggaaaagac cggcaaggtc cccccggacc
     7981 tggtcaaggc cttgggcgaa gcggaaatga tggccatcac catccccgaa caatacggcg
     8041 gtttgggact cgggttcgtg gcccgaatga tcgccctcga agagatcagc cggatttccg
     8101 ttgccacggg aatgatgctc caggttttgg ccttgggaat cgaccccatc gtgcgattcg
     8161 gaaacgaggc ccagaagaag aagtacctcc ccggcttggc caggggcgaa cggctcgcga
     8221 ccgtggcggt caccgaaccc acgggaggtt ccgatcccac gggaggccgc accgtgtact
     8281 ccaaggacgg gaccgattac gtcatcaccg gccgcaaatg cttcatcacc aactcccaca
     8341 tcgccgatgt cgtgaccctt ctcgccaagg acgaaaacga tcccaaagca ttttccgcgt
     8401 ttatcgtcga gaagggcacc ccggggttca aggccaccca cgaggaacac aaggtcggga
     8461 tgaaggggtg caataccggg gaactgagcc ttgaaaagtg ccgggtgcct gcggaaaacc
     8521 tcgtgggccg ggaggggaaa ggactgcgag tcaccatgtc ggccatcgga gacgtggggc
     8581 ggggcggcat ggtgggttgc gctctggggc ttcataccgc ctgtctcgaa gcctccctca
     8641 agttcgccaa ggaccggatc ctttatggaa agcccatcag cgtcctccag accatccaaa
     8701 acaagctggc cgaaatgaag atcgacctcg aggccggacg gctcctggcc tatcgcgccg
     8761 cagccatgca ggaccgggga gagcccagca gcaacgagtt cgccgtagcc aagtacttca
     8821 ccaccgagac cgcccaaaag gccgccaaga tggcagtgga catccacggc gggtacggct
     8881 gcatggaaga atacgccgtg agccgctacc tgcgggatgc ctttgtcctg ggaccttccg
     8941 ccggaaccag cgacatcatg aaagtgatca tcgcccggtg ggttcttgcc tgatccggaa
     9001 atctccggcg accgtcctgc gtcggctgtg gccggagact cccattctcg aggatgcgat
     9061 gaagatcgtc gtttgcgtca aggccgttcc ggaccccaag gagatctgca ggattaggat
     9121 cgaccccgtc tccaagactc tgacccggtg cgaggtgccc ctcgtcgtca atcttctgga
     9181 caaacatgcc ctggaagccg cccttgccat caaggaatct tccggggcct ttgtgaccgt
     9241 cctcagtatg ggacctcccc ccgccgaccg catcgtcaag gaatgcatga gcctcggggc
     9301 cgatcggggc attctcttgt ccgaccgggc ttttgccggg gcggatgcat ttgccaccgc
     9361 ccataccctg gcccgaggca tcgagaaggc aggccccttc gacgtgattt tctgcggcat
     9421 ggcgagcagc gatggggcca ccgagtgggt tgggccccag attgccgcct tcctggacct
     9481 tcccgtggtg accatggtcc gaaagttcga atcccgccag ggcgatcgat ggctcgtcct
     9541 ggccgattgg cagaacgggt atcgaagggt ggaggttcgc ctgcccgccc tgttcaccgt
     9601 cacccgggag ttgaatcgcc cccgtgcttt gagcttttcc gggattctca aggcacgcag
     9661 caagaccatc gaacactggg acctctcctc tttgggagtc tcggccgatt ccgtgggggc
     9721 gcaaggttct cccaccctcg tcaccgaaat ttccccggtt gaaaatcggc gggatgcgga
     9781 aatgctccgg ggaaccctcg aggaaaaggc cgatcaattc gtctcccgcc ttgcggagct
     9841 gggaatcgtg tagggaagga tgccccatga agaagcgctt gggtccggtg tgggtgatgg
     9901 cggaacaaag gggctgcgag gtggagaccg tatcccttca gctcttggga aaagcaaggc
     9961 ggttggccga tgagctgggc gtggcgtgcg aatccgtgat cctgggccac aacttgggag
    10021 aagcggcccg gaggctcatt gccgggggcg ccgatcgcgt ctttgtgggg gacgctcccc
    10081 acctggccct gtatcagtct cttccctacc gggacattct ctgtgccctg gtcgaggaac
    10141 ataaacccga gattctcctc ctgggatcga ccttcctggg ccgggaactg gcaccccttg
    10201 cggcggcccg gttgaaaacc ggcctggccg cccattgcac cggattgcgc catgacaagg
    10261 acgggaacct cgagcagcaa attcccgcct acggggggtt gatgtccatt gtgtgcccca
    10321 accggcgtcc ccagatggcc accgtcgcca agggagtgtt caaagcggcg gagcccgatg
    10381 agaaccgaaa gggggaaatc gtgcccgttc gagtaccggg agagaccgaa ctcggggtga
    10441 ggaccctgga aatcgttcaa gaagcgccgc cggaaatcga gctcgaggaa gcggacgtgg
    10501 tggtttgcgg gggtgccggg gccggaagcg cggaaggatg ggaaatcgtc cgggaactgg
    10561 ccgtggcgct gggagccgcc ctgggatgca ctcgaccggt ggtggacgaa ggatgggccc
    10621 ctttggacgc catgatcggt caaagcggga aaatggtgag tccccgggtc tatatcgggg
    10681 tgggtctctc gggggaacag cagcacatgg tgggggttgt gggcgcccaa ttcatggcgg
    10741 ccgtcaacca ggacccgaaa tccgcgattt tccagcaagt cgacctgggc gtcgtcgagg
    10801 attgccgcaa attcctgccg gtcctcatcg agaagatcaa ggctttccga aaagaaagaa
    10861 ccttctgcag gcctccctcc gtttcgtagg acaattcatg gacatggggg tcggctcccg
    10921 cctccagaga cgttcgggct tatccgaaaa caggggccgc ctgcagtctt cggacaggcc
    10981 cccggtattc ggcggggcaa cgtccgtccc caagacagag agaatggagg ggaaacgagc
    11041 atgggcgata tgaagaagct cgccgaactg gtcaaggaac tccaaggccg gttggcggtc
    11101 tgcatgcgtt gcggcctgtg ccggtcggtc tgcccgattt tcgcacaaac cgggagggag
    11161 ccggacgtgg cccggggcaa gcttgccctc ctggacggaa tcatcgaagg ggtgttccgg
    11221 ggtccccgcg gggtgaatga tcaacttcgg cgctgcctcc tgtgcggcgc atgcggtgcc
    11281 aactgtccca gtggggtaaa gaccctcgag ttgtttctca aggctcgggc cctcctggcg
    11341 ggttacctga agctttcccc tctcaagagg ctcctgttcc gcgccctgtt gccccacccg
    11401 aaggtttttg acaggctgct ggaatggggg tccggcctcc agggcatttt cctgaaaccc
    11461 gtggacgatt ttctaggaac gtcctgcggg cgggtgccgt cgcccctgtc gggccgacgt
    11521 cgcctgaagg cgcctgcttt ggtccccttt caccgcaggg tgtcgtccct ggatcggcct
    11581 gcaggaaagt cgggactccg ggtgggcttc tttgtggggt gcctcatcga caaggtattt
    11641 cccaacgtgg cggaggcgat cctggaggct ctcgaaaagc acggagtggg agtctttctc
    11701 cccggggacc tgggatgttg cggcatcccc gccctgtccg ccggggacgc gaccgctttc
    11761 ggcaggctgg ttcgccacaa cctcgaaaag ctgacttccg aaccctttga ttttctggtc
    11821 acaggatgcg ccacctgcac cgccaccatt cggaacctgt ggcccgtcct gtacccggac
    11881 atcgaggacg gaaagagggc ccaggtccgg gccctggcgg cgaaaactct ggacatcagc
    11941 cagttcttgg cggacaaggc gggacctctt cacgggaccg gaccgacctc aggagatgcc
    12001 gtaccccttg cctaccacga tccctgccac ctggcgaaat cgttgggagt gacggcccag
    12061 cctcgagccc ttctaagggc caacggcctc taccggttcg aggagatggc cgaagcggat
    12121 gaatgctgcg gatgcggagg cagcttcaac ctcgagcatt acgatctttc cgcctccatc
    12181 gggagacgca agcgcgaaaa tattgcccgg tccgggtgca aaacggtggc caccggctgc
    12241 ccggcctgca tgctgcaact gtccgatatg ctttcccagg cgggggaccg cattcgagtc
    12301 aaacacgccg tcgagattta tgccgaagtc ctgaggccgt aagcaccgct cccgcccata
    12361 cggacggact acagggaggc cttcccggcc gtcacccggt ctccgtggtg cggagtccgt
    12421 ctcggtttgc cgtttgccga ctccccggct gccttcccgt tgcgtctttc ccgggcaagc
    12481 ctgccgacac cggcttggct cccgccgcct ccgatcccct gccctcccgc gctcctgggt
    12541 cggtgccgtc cttgctttcc cgggacgttc gatcccgccg ggcgggcctt gccctttccg
    12601 gacccgttgt gccggtgaac ggggtgttcc ggctccaatc cgggaaccac atggacggac
    12661 aggttgtgcc cgagatagtg ttcgatgcga tccagatacg ggagatcggc ccgggatgca
    12721 aaggaaatgg cggtgcccga ttcccccgcc ctgccggtcc tgccgatacg gttcacgtag
    12781 tcttcggggg aacggggcag gtcgaagttg atgacatggc tgatgcccgt gatgtccagg
    12841 cctcgggcgg caacgtccgt ggcgaccagc agccggatct tgccccggcg catgttcgcc
    12901 atggtgcgat tgcgagcggc ctggctcatg tccgcgtgca gagctgcaga ggcatatcct
    12961 tgctggttga gttgtcgggc caggctgtcc gcgtcccgct tggtggccga aaagatgatg
    13021 gcccgggtca aggacgggtc cttgcacagg tgccccaaca gtcgattctt gtggaccagg
    13081 ttgtccgcca tgtgcaaccg ctgttcaatc cggtcatggg tgatcttacc cggggcaatc
    13141 cggacccgat ccgggtcgtt catgaatttt tgagccaacc gggcggtcgc gtcccccatg
    13201 gtggcggcaa acaagaccct ctggcattct tcgggcacgg cgtccgagat gaattctacg
    13261 tcatcgatga agcccagttc cagcatccga tcggcctcgt ccaggacgag cagttccacc
    13321 tgggaaagca ggatccggcc gcgccagatg tgatcgatga gccgtcccgg cgtggcgacg
    13381 atcatgtccg ggatttgggc cagggcccgt atctggtctc cgaagggcat tccgccgtaa
    13441 atagccacgg aatggacgtt ggtataccgt ccgtaggctc gcgccgcttc ggtgatttga
    13501 gtggccagct cgcgggtggg agtgagcacc aggatgcgca cgccggtttt cgaggggaga
    13561 ggattggtcg tcaaacgctg gagggcgggc agaacaaaag cggccgtctt tccggtgccc
    13621 gtttgggccg aagcgaccag gtcgcgtccg gttaagacct tgggaatggc ttccgcctgg
    13681 acgggggtcg ggtgatggtg tttacaccgg gaaagggcct tgaggatagg gggggtcaaa
    13741 tgaagctctt caaaagtcat acgctgatac atccttcatg gttgccggat tcgagcgggt
    13801 ccccccgggg acttgctgcg ctcgaaacag cacacgttcg tgggtaaaac aagctgcagg
    13861 ggatagcccg cggaggagct tgaaactgca gcatccaaat tggatcggcc tgcgacatcg
    13921 ccgccaacca atcgcgccgc agcaatgaaa aagattgctg ttgtcaaggg gttagggtcc
    13981 gatggggcta acgtggcggg ggtaaaaagg gaaggactcc ttcacttccg ttcccaccat
    14041 ggggccttgt gatcagggat ataaacattt tttcgccggg gagtcaaaca acaaccggac
    14101 cctgttttct cggcatgttc ccggaagggt gcttcatctc gcccgcactc cccggtatct
    14161 ccaccgggaa agcggcagtg cttcggtctt agaaacgtct tcctcctccg cctcccccgc
    14221 cccctcggcc ccttccgcca aagtgtccgc cctccctgcg gccgccgcct cggcgatctt
    14281 cgcggggttt tgcttcgttc actctcagga cggagccgcc aagttctttg ccgttcagcc
    14341 cttccattgc cgcctttgcc tcggccgtgt tcggcatttc cacgaatccg aaccccttgg
    14401 acttgccggt gtagccgtcg gtgatgatcc tgaccgatcc cacctcccca aatactgcga
    14461 aggcctcttg gagctctctt tcggttgtct tgaaggacag gttgccgacg taaatgttca
    14521 tggaaacccc tttcgttgag caagagacaa ctttgtgccc tatggagacg ggagcatgca
    14581 cgggtccgag cacttcccgg ctccggggga ccatctatgc caatcctgtg gacaccacct
    14641 caatctcagg agacctttgg ctgagccggc gagatggaag ggtactgtgg ggtggcaggc
    14701 gggaaaggcg gtatttgccg cgattcagga agaagccacc aggcccttgg tcacactcac
    14761 cttaaagcac tcgggcggaa atggcaatcc ccaaacggga ggcgaccctt aatcgctgtt
    14821 gacaggcatc ctcatcgagt cctatgaaag agcctgtttg acgacgggaa ccatcggacc
    14881 gccgctttcc cggtcgagga cctcgggggg cgaccgaggg aagagcgccc cgacttccgc
    14941 gagaaaattc aggccggacc catgacgact tgcgacaaga aacgatccta tcgacagctg
    15001 acgttttccc ccctccgggc cgttttccag agatgcctct ggtgttgcga ccatcagagt
    15061 gccgaagtcg ccgcctgccc ctcggtgaaa tgccccctgc atcccttccg cctgcggaag
    15121 gctccgggct cgaaccggct tctgctcaag gtcattcggc aaaaatgcct ggaatgtgtc
    15181 gcagggactc gttcggaggt cgaaaaatgt gcctccgtcc agtgcccgtt gtgggaattg
    15241 cggcttcccg gggacaaact tcctcctccc gggtcgtctc ctgcgtcttg gaccgaaccc
    15301 ttttgacccg ctcccccatc cacccgtttc ttcccggact tctttccacc ctctccccag
    15361 gaacggcctt ttgtcgataa ggcccatcga cccccccgca cagaaatgcg tgtccgggga
    15421 ggggcggagt caccgtcaag acctaatctg accgattccg agagcatccg cccgtcatgc
    15481 ccgccaaggc gggcatccag aggttccgca ccctcttcat ggatttccgc ttgcgcggga
    15541 atgacgagag gcgatcgact ttttccggca gaaggtcagg ctcccgagcc ggacctggac
    15601 cgaactctgg gagggcatgc acagccgggc attcgtggtg gccggcgcca tgaaagacga
    15661 cctgctctcc gattttcagg ccgccgttct tatgggcatc gagaagggga tcactctcaa
    15721 cgaatttcga aaagacttcg acagcatagt gaagcggcac ggctggagct acaagggcgg
    15781 gcgcggatgg agatcttcgg tgatctttaa accgaagttc gtaaagaatt tctatctgac
    15841 agctcctaaa tgattgtaac atttaa
//
LOCUS       DAOOVC010000091        15748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2500_length_15748_cov_6.464793, whole genome shotgun sequence.
ACCESSION   DAOOVC010000091 DAOOVC010000000
VERSION     DAOOVC010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15748
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2500_length_15748_cov_6.464793"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLM79_11765"
     CDS             <1..526
                     /locus_tag="PLM79_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOV87032.1"
                     /translation="LGARHLFPQARTVLDIGGQDTKVISLSSQGKVMKFEMNDRCAAG
                     TGKFLEFMATALQVPLESFGEFARRADKRITINSMCTVFAESEATSLMARGERPENIA
                     MGLHLAVFQRTLAMLRRVGLQMPLVFAGGVAHNECAVRILEELLGEPVMVPEDPDAVG
                     ALGAALWGAKARVP"
     gene            523..2562
                     /locus_tag="PLM79_11770"
     CDS             523..2562
                     /locus_tag="PLM79_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV87033.1"
                     /translation="MSERPTLCRVCSENCGILVKRDRRGTRIRGNPDHPVSKGFLCFR
                     GKNYGEVHDANTRLDSPMVRSGSHWTKVSWEDALGLLAERLLDARERHGPQSVVFYKG
                     ESLKHQEIARYMQHLSFGFGTPNYVSVGSLCHHSSAFSHALTYGGTAQPDLHRIRLAI
                     LWGTNPAISAPRTFLDLKKAAGRGMKLIVVDPSRTQSASHADLHLSVRPGSDGFLALA
                     FLKHALEEETVSPELASSDGWEELNRLLRRVSFGSLLEETGIEVEEFRNAAAMIFENT
                     PGWISTGLGLELQPNGFQSLRAIACLQPVLDPRNRAAGFRAPLKPLPGSDRYPPMPPS
                     IGSQRWPLYTGSLREGQGMQLTRAILRDDPYPVRAMLVAGGNPVLTFPGTRFQTAAYE
                     KLDFLAVVDLFMTPTARKAHLVLPAATFLETVELHDYGVVGKPYLGLVQPVAPSNKGR
                     PAWQWIFDLARRLELSALFPWKDNREALMGALSGTGVTLEDLEKSASAVLPYSPEVSP
                     ETTWRTRTGKVQFYSKELAALGYSGLPVPEDLRLPVSTGAPFPFWLSTGDRVLGFQHT
                     QFRNAPTAGNLMKKPILDIHPDSAARLGVRDGEKVAVTSRYGRLEIRAALCPEVRRDS
                     LRMSHGWESCNANDLTDSEYFDPVTGFPWMRALPVSVEKIGERSRGRRDGPSPSK"
     gene            2784..2999
                     /locus_tag="PLM79_11775"
     CDS             2784..2999
                     /locus_tag="PLM79_11775"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOV87034.1"
                     /translation="MRFGARNKIKAKVVSVKKGDVMSLIKFDVDEPVHMASVLTTESV
                     EDLNLGVGDEVELVIKAIHVLPVKKGS"
     gene            3888..4748
                     /locus_tag="PLM79_11780"
     CDS             3888..4748
                     /locus_tag="PLM79_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV87035.1"
                     /translation="MKDKPECGTRRTFLKSALMATIGAALPTGMARAGAAFSQDSLQV
                     WSCGGLAEAFMPANEMYEKRAGVKISYTGAFAAALGKSLLGSATTEVFGGRVLDLAKK
                     LRQTGKMVYFRPLCFTSYVMVTPKGNPAGIREIKDMAKPGVRVVLAPDASPPGGQAAQ
                     GVLKKAGILDAVMKNAVTLGSCVQRTMDDIVGGKGDVSIVELRITRMPAFEGKMDIVC
                     IPEDYFPPPPLTFTIGVMKDAKDRALADDYVDFITSNEGQSFFDRCGFIPAVSDKGRE
                     LIEKLGVKDV"
     gene            4741..5547
                     /locus_tag="PLM79_11785"
     CDS             4741..5547
                     /locus_tag="PLM79_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV87036.1"
                     /translation="MSNAEIAVPKNLSRAHGAISTIRLTFFRRLSQLALIGVLGQWSF
                     YGIFRCPFLVPYISCRNCPVITCHGRILTMFWGFWLLLPVSVLLFGRAFCGWACPGGF
                     VNQMLGKLAPLKLRVRNVFTRFAPFGQYLGLAVALYLFFALGQPRADVPIRVGEFFKS
                     VALTFEHAGLLWLVRSFFVLGFLVLGLIAANSWCRFACPTGSLLEALKHLSLFRFYKT
                     DQCNHCNQCLKACEMGTRPDEMNCTNCGDCMSACPTDAIRFGRKKPSRDV"
     gene            5540..6511
                     /locus_tag="PLM79_11790"
     CDS             5540..6511
                     /locus_tag="PLM79_11790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87037.1"
                     /translation="MSESLCSNDGVLRHPCFHPGARSRYGRVHLPVAPTCNIQCSYCN
                     RAYDCTNESRPGVTSRTLEPEEAAAYLDEVLYRMPFISVAGIAGPGDCFADPGRTLAT
                     LEIIRRSHPNLHLCFSTNGLNVAEHMDALAELNVRFATVTINAVDPRVGAMIYTRVAG
                     GDVALQGLDAAAFLMERQMDAVARLKARGLIVKVNTVVIPGVNDIHSAAVAERIVRLK
                     ADLHNLIALIPVPGTPFQGTAPPSPERMTLLRRAAEAFIPQMRHCVRCRSDAVGLLHD
                     DRSCMGAHPALEPQAAGTSAGSGTCSVDLRVVGGNGSLRDVHRLSMP"
     gene            complement(6797..7270)
                     /locus_tag="PLM79_11795"
     CDS             complement(6797..7270)
                     /locus_tag="PLM79_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase P"
                     /protein_id="HOV87038.1"
                     /translation="MLEIHIPGDDRPMNLSHLVLDYNGTVACDGRLISGVRERLETLS
                     AGLEIHILTADTFGSVHEALAGIPCRLSIIPRQEQAQAKLRYVERLGPNSCVCVGNGR
                     NDERMLREAVLGIAVVQTEGAAVTAVLAADVVTSGILDALDLLLHPLRLMATLRS"
     gene            complement(7293..7997)
                     /locus_tag="PLM79_11800"
     CDS             complement(7293..7997)
                     /locus_tag="PLM79_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV87039.1"
                     /translation="MVLNSVDLALCPGELTVIVGPSGSGKSTLLRAVNRLNECFPGCR
                     TRGEVRIRLDGRWGNVYDDFLPLPELRRKAGMVFQTPGVLPFSIRKNLAMPLKTTLGI
                     AGSVLAERIERALREASLWEEVKDRLDDAATTLSGGQQQRLCLARALALEPQILLLDE
                     PTASLDFRAARKIEQLLTSLKQRYTVLAVSHSLGQTRRLADKVYVLREGVIGQEISGE
                     CLQDPKTFEKLIEDAF"
     gene            complement(8057..8935)
                     /gene="pstA"
                     /locus_tag="PLM79_11805"
     CDS             complement(8057..8935)
                     /gene="pstA"
                     /locus_tag="PLM79_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOV87040.1"
                     /translation="MKKNLDFLMTGYARLCAVAVFLATSLLLGFLIKRGLSSVDLKLF
                     FGDTHWLSAITGTQPVFEGIWPAVVGTLFLVLLSSAISIPTGVASGVYLSEYASGRVR
                     SFLGFAVDVLSGTPSIVMGLFGFTMILFLRNTLLPQARPSLFLAAVCIALLILPYVIR
                     TTQTALEAIPEHLRLLGPSLGLSHWHNIRYLLVPLAGRGILSGVILAIGRAAEDTAVI
                     LLTGVVARAGLPASLWDKFEALPFRIYYLAAEHRSQAELDQGFGAALVLLLLTGFLFL
                     AAFFLHAKFEKRWKTH"
     gene            complement(8919..9731)
                     /locus_tag="PLM79_11810"
     CDS             complement(8919..9731)
                     /locus_tag="PLM79_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOV87041.1"
                     /translation="MAILFMLALLVYFCLPLITSDGMSAVLSWEWKPFSGHFGILPMC
                     LVSLLLSVFALGLAFPAAIGICGFVTVLAPRGLARFFLILIHFMTSIPTVIYGFVSVF
                     LLVPLVRDRLGIGTGFSFLTAGLTLSLLILPTIVLIFHARLQQIAPSLRLTAEAMGLT
                     PARQFLHVLIPASSRGLIVAAVMGFGRAVGDTLISLMVAGNAPQIPSSLFDSVRTLTA
                     HVALVVATDSQSLSYRSIFASGLILFLLMGGANFLIRRLDDGIGKDRHEEEP"
     gene            complement(9844..10689)
                     /locus_tag="PLM79_11815"
     CDS             complement(9844..10689)
                     /locus_tag="PLM79_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOV87042.1"
                     /translation="MGILRKRMNVVAVLSVFFLLGWFRSAALCGALDGFAGLEGTIGI
                     AGGTAHIPVMNDAAKAIMTFNPKIRITVEGGGSGVGVQKVGEGLVDIGNTGRAVSQEE
                     IAKYGLKSFPFALDGVAVAVHPDNPVDNLSQQQVRDIFAGSITNWKDVGGKDGSIHLF
                     CRDEASGTREVFWEKLLKKGPVAGTANIVSSNGAMKMAISRDKDAVGYVGIGHVDQSL
                     KAVKIESVAATQENASNGSYPVVRKLYMNTKGEPARLTKAFIDYILGPEGSEIIRRHG
                     YIPLE"
     gene            complement(10998..11486)
                     /locus_tag="PLM79_11820"
     CDS             complement(10998..11486)
                     /locus_tag="PLM79_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87043.1"
                     /translation="MIRNIHLDPTLKKQLKVLRKAGGRGAAAAVHARNIIDQLLSDVR
                     RNLNQVGRMTRYGEARIKNCVKYDLVRGYRLIGILQGEDLFFVFLGSHDECDHWLRNN
                     AGIENIPSKRRSSTLSVPPEDPDPRESPRVQEPEALEDEILFREIDERDLRIIFRGLC
                     KA"
     gene            12265..13215
                     /locus_tag="PLM79_11825"
     CDS             12265..13215
                     /locus_tag="PLM79_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV87044.1"
                     /translation="MSLLLSGCGEPNPRLYRVGILSGLDTFAATVDGFKAKMTELGYI
                     EGKTIAYETERTNFDQEAEERILRRFVDERVDLILVFPSEASVAAKEITRGTSIPVVF
                     CQTNIEGTDLVRSVLEPGENLTGVRFPGPDIAVKRLEVLLELAPRAKRIWVPYGRNVP
                     IVPDQLTALRLEAARGGIDLVEAPADGADDLLADLEMREKTADPGIDAILLISEPLAK
                     TPTVFPTMIRFAYRHRIPVGGFAISHEGHSTVFGVATDNLAVGRTAAIQAHKVLRGIP
                     AGTIPVVSAESYFHINYRAAQELGLSVPEGLLRQANEIVR"
     gene            13273..15699
                     /locus_tag="PLM79_11830"
     CDS             13273..15699
                     /locus_tag="PLM79_11830"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HOV87045.1"
                     /translation="MKTRVETFSSGRKLSTTLAGAFFLFSAVVLLLYTGLMGLYDYRT
                     LEALISSRQQLIARDAAKAVSGFIHDNFSVLETAIWLTDFDTQREVERRRILQSLLGL
                     RPAIRRLVLLDPHDQVLARASRLSMQASGQFTDRRLEELLSGQDRKAKRRMSTVTIDP
                     VTGEPLVWLAVPVTDAFEDVRGTLIAELNLKSMWDIVDQLRVGETGYVYVADREGNLL
                     AFQDTSRVLKGGRVSHIRVVADFIRNKPAVQPVAVERFQGIKGSTVVGTYAPLTMPDW
                     AVVTELPWEEAYRVMARNLAAAVAFTLGIAILAGIFGVRVARRMAKPVIQLTETASRI
                     AAGEPDLKAEVGGPREVACLAVAFNSMTSQLRQSLVDLEDRFGELKRTEEALRLSEQR
                     LRLALEATSDGIWDWNLETGRVYFSPRYYTMMGYEPDEFPASYENWKRWVHPVDLEAA
                     EKLVWQAIQDRTSYTIEFRFRAKDGDWRWILARGKVVETDEAGKAVRVSGTHSDITPR
                     KRVEEALEKRILALTQPLGSVENIALEDILNLSDLQHLSDLFAKAFGVASLITRPDGT
                     PITQPSNFTSFCSEIVRKTPRGLEKCNHSDAIIGRHNPSGPNIQPCLSAGLCNAGASI
                     TLGGSHVANWLIGQVRNEGVDEEKIMEYGREIGADEGVFRAAYLEVPVMSQEQFDNTA
                     HVLFAVANQISNSAYQNILQARFISERKQAEESLRKYERIVSSSQDVMALINTDYVYE
                     TVNESLLKLQGRSREEIVGRTVSKVMGEKTFRHKLKARLDQALSGQTVHFQESFDYGE
                     TGRRVMDVNY"
BASE COUNT         3286 a         4473 c         4816 g         3173 t
ORIGIN      
        1 cctgggggct cgccatctgt ttccccaggc ccgcacggtt ctggacatcg gcgggcagga
       61 caccaaggtc atctccctgt cgtcccaagg gaaggtgatg aagttcgaga tgaacgaccg
      121 ctgtgcggcg ggcacgggaa aattcctgga attcatggcc acggccctcc aggtgcccct
      181 cgagtccttt ggggagttcg cccggcgggc ggacaaacgc atcaccatca acagcatgtg
      241 tacggttttt gccgaaagtg aagccacctc cctgatggcc cggggggaac gtcccgaaaa
      301 cattgccatg ggactgcacc tggccgtctt tcagcgcaca ctcgccatgc tgcgtcgtgt
      361 gggcttgcag atgcccctgg tctttgccgg gggagtcgcc cacaacgaat gcgccgtgcg
      421 gatcctcgag gaactgctgg gagagcccgt gatggtgccc gaggatccgg atgcggtggg
      481 ggcgttgggg gctgccctct ggggagccaa agcgagggtc ccgtgagcga acggcccacc
      541 ctatgtcgtg tctgcagcga aaactgcgga atcctggtca aacgggatcg gaggggaaca
      601 cgcatccgcg gaaacccgga ccacccggtc agcaagggtt tcctgtgttt ccggggaaag
      661 aactacggtg aggtgcacga tgcgaatacc cgcctcgatt cccccatggt acgcagcggg
      721 tctcactgga cgaaggtttc ctgggaagac gccctgggac ttcttgccga gaggctcctg
      781 gatgctcggg aaagacacgg gccccagagc gtggtatttt acaaaggcga atccttgaag
      841 caccaggaaa ttgcccggta catgcagcac ctttcgtttg gcttcggaac ccccaactat
      901 gtctccgtgg gcagtctctg ccatcattcc tccgccttca gtcatgccct cacttatgga
      961 ggaacggctc agccggatct tcatcgaatc cggctggcga tcctgtgggg aacgaacccg
     1021 gccatatcgg ccccgcgcac ctttctggac ctaaagaaag cggccgggag agggatgaag
     1081 ctgatcgtgg tggacccatc ccggacccag agtgcttctc acgcggatct ccacctttcg
     1141 gttcggcccg gttccgatgg gttcctggct cttgcttttc tcaaacacgc cctggaagag
     1201 gagacagtct cgccggagct cgcgtcttcg gacggctggg aagaactgaa ccggctcctg
     1261 cgaagggtct ccttcggttc tctgttggaa gaaacgggga ttgaagttga ggagttccgc
     1321 aatgccgctg ccatgatctt cgaaaacact cccgggtgga tttccacggg actgggactc
     1381 gaacttcagc cgaacggctt tcagtccctc cgggccattg cctgtctgca gcccgtcctg
     1441 gatccccgga accgtgcggc gggattccgc gctccgctca aacctctccc cggctcggat
     1501 cgatacccgc ccatgccgcc ttccatcggg tcccaacgat ggcccctgta caccgggagc
     1561 ctcagggaag gtcagggcat gcagttgacc cgggccattt tgagggatga tccctatccg
     1621 gtgagagcga tgctggtggc cgggggtaat ccggtgttga cctttcccgg gacccgcttc
     1681 cagacggcgg cctatgagaa actggacttc cttgcggtgg tggacctgtt catgaccccg
     1741 acagcccgaa aagcgcacct ggtgctccct gcggccactt ttctggagac cgtggaactc
     1801 catgactacg gcgttgtcgg aaagccttac ctgggactgg ttcaaccggt ggccccctcg
     1861 aacaagggac gcccggcctg gcaatggatt ttcgacctgg cgcgacggtt ggaactttcc
     1921 gcgttgtttc cctggaagga caaccgtgaa gcgctgatgg gagccctttc gggcaccggt
     1981 gtgaccctgg aagatctcga aaagagcgct tccgccgtcc tgccctactc gccggaagtc
     2041 tcccccgaga ccacctggcg gactcgaacc ggcaaggtcc agttttactc gaaggaattg
     2101 gccgccttgg ggtactccgg actgcccgtc ccggaagatc tgcgcctccc ggtttccacc
     2161 ggggctccct tccccttttg gctgagcacg ggggatcggg tgctcggctt tcaacacacc
     2221 cagtttagga acgcccccac tgccgggaac ctcatgaaga aaccgatcct ggacattcac
     2281 ccggacagtg ccgcacgttt gggggtccgg gatggggaaa aggtggccgt gacgagcagg
     2341 tacgggaggc tggagatccg ggcggctttg tgcccggaag tccgtaggga cagcctgcga
     2401 atgtcccatg ggtgggagtc gtgcaacgcc aatgacctga ccgattccga atactttgac
     2461 ccggtcacgg ggttcccctg gatgcgggcg ctccccgttt cggtggagaa aatcggagag
     2521 cggtcgagag gtcggcggga cggcccgtct ccctccaagt aggtcccgga ttgggcgatt
     2581 ggaccggggg ggatcctgtt tcgtcattcc cgcgatgcgg gcaaccggaa agagcgtgca
     2641 tacccgccgt cgccggcatg acgccctgat gctttcggaa tcgctcaact caagcgatag
     2701 tgacaatgga tttcgggatg cccatgatcc cggggtctgg gccgtttacc gtgagagaag
     2761 aactcggagc gaaggaggag accatgagat tcggtgccag aaacaagatc aaggcaaagg
     2821 tggtttcggt gaagaagggg gatgtgatgt ccctgatcaa gttcgatgtg gacgaacccg
     2881 tccatatggc gtccgttctg actacggaat cggtggaaga tctgaacttg ggtgtggggg
     2941 acgaagtgga gcttgtgatc aaagccatcc atgtgttgcc ggtgaagaag ggatcgtaga
     3001 aaccctgagt ctgccggaat caatccccgg cttgttgtcg tttgcgtcgg cgacggagga
     3061 cccctcgacg cgcccgttgt gggcgtaatg ggaacgggcc caaggcctta ggctgcagaa
     3121 gccgatcctc cgaaccacaa cggagcggcg gataagccgc gacgggcggg aaggccgtag
     3181 gtttttccgc ccggagtcgg cttcatctgc gggttgcgcg gcgcgaagcg tccgcgacaa
     3241 cttcgttttg gggggaacac ggacatcctg gtcaaagagc ctggattgag cgattcggcc
     3301 gggacgaatc ccggttcctc atccctatga aagcgaaact ttcatgcaac ggggcgcggc
     3361 ccgttgcatg aaggattccc gcgcaggcgg gcatccacga agagcgtgtc cacccattgg
     3421 agatgtccgc cgtcgcgggt acgacggcct gatgctccgg gaatcgctca acgcgggaga
     3481 gggagattct tgtcgttgga gcctgaacag gggacggctt cctccggatg atgattccaa
     3541 caatatccac tccgtatcac cgaacaaaga aatctcacgt caaacggcta ttttctcagc
     3601 aattctcaag gaatcgcccc cggaaggggc cctcgggaat tgtccatttg atgaattcta
     3661 tggattgaac ctcggaagac ggcagaaggg gcggcaaaaa taaccgattc acacaatagg
     3721 catcggcgat cctacggcga tgtcccagcg gggaaaaccg attagacact gagtggttcc
     3781 ggatgctatc agagcggcct gatgtcggtt gttgtctgca tgcatccttc tgagcggccg
     3841 gggggtgatc tactgttcat actgggagag acgggaggag tcggcgcatg aaagacaaac
     3901 cggaatgcgg aacacgcagg acttttttga agtcggcgct tatggcgact atcggtgcgg
     3961 ccttaccgac gggcatggcg cgagcgggag ccgccttcag tcaggattcc ctgcaggtgt
     4021 ggtcgtgcgg cggacttgcc gaggcattca tgccggcaaa cgagatgtat gagaagagag
     4081 ccggggtgaa gataagctac acgggggcct ttgcggcggc cctgggaaag tcgctcctgg
     4141 gaagcgccac gacggaagtc ttcggaggca gggtcctcga tcttgccaag aagctaagac
     4201 aaaccgggaa gatggtttac ttcaggcctc tttgcttcac gagctatgtg atggttacgc
     4261 ctaaggggaa cccggcaggg atccgggaga taaaggacat ggccaaaccg ggtgttcgcg
     4321 tggtccttgc cccggatgcg tcgcctcccg gaggccaggc agcccagggg gtcctcaaga
     4381 aagccgggat tctcgatgcc gtcatgaaaa atgccgtgac ccttggaagc tgcgtacagc
     4441 gcaccatgga cgatatcgtt ggaggaaagg gcgatgtctc catcgtagag cttcggataa
     4501 cccgaatgcc tgctttcgag ggcaagatgg acattgtctg catccctgaa gactatttcc
     4561 cgccgccgcc gctcaccttt accatcggcg tcatgaaaga tgcaaaagac cgcgctcttg
     4621 cagatgacta tgtggacttc atcacatcga acgaaggtca gtcctttttc gatcgttgcg
     4681 gcttcattcc ggccgtctcc gacaagggcc gggagctcat cgaaaagctg ggggtgaagg
     4741 atgtctaacg ctgagattgc cgtcccgaaa aacctgagcc gggctcacgg agcgatttcc
     4801 accattcgcc tgaccttttt tcgaagactc agccagcttg ccttgatcgg cgtgctcggg
     4861 cagtggtcct tctacgggat cttccggtgt ccctttctgg ttccctacat cagttgtcgg
     4921 aactgccccg tgattacctg ccatggccgt atccttacca tgttctgggg tttctggctg
     4981 cttctgccgg tttcggtgct tctcttcggg cgtgccttct gcgggtgggc ttgtcccggg
     5041 gggttcgtaa accagatgct cgggaagctc gccccgttga agcttcgagt gagaaacgtt
     5101 ttcaccaggt ttgcaccctt cggacagtat ctggggctcg ccgtggccct ctacctattt
     5161 ttcgctctgg gtcagccccg agccgacgtt cccattcgtg tcggcgagtt cttcaagtcc
     5221 gtggctctta ccttcgagca cgccgggctc ctctggctgg ttcgatcctt ctttgtgctt
     5281 ggtttccttg tcctggggct catcgcggca aactcatggt gccgctttgc gtgccctacc
     5341 ggcagcctgc tggaagctct caagcatctt tctctcttca ggttttacaa gaccgaccag
     5401 tgcaaccact gcaatcaatg cctcaaggcc tgtgagatgg ggacccgccc ggatgagatg
     5461 aattgcacga actgcggcga ttgcatgagc gcatgtccaa cggatgcaat ccggttcggg
     5521 cgaaaaaagc cgtcccgaga tgtctgaatc cctgtgctcg aatgacggtg tcctgcgtca
     5581 cccttgtttt catccagggg cgagaagccg ctacggccgt gtccatcttc cggtggctcc
     5641 aacctgcaac atacaatgct cttactgcaa cagagcctat gattgcacaa acgagagcag
     5701 gccgggtgta acaagcagga ctcttgagcc ggaggaggct gcggcctatc ttgatgaagt
     5761 gctctatcgc atgcctttca tctcggttgc aggcattgcg ggtcctgggg attgctttgc
     5821 cgatccgggg agaactctcg caacactcga aatcatccga aggtcccacc ccaacctgca
     5881 cctttgtttc tcgacgaatg ggttgaatgt agccgaacac atggatgcgc tcgcggaact
     5941 gaacgttcgg tttgcgaccg tcacgatcaa tgcggttgat ccaagggtgg gtgcgatgat
     6001 ctacacgcgg gttgccggag gagacgttgc gcttcagggg ttggacgcgg cggcttttct
     6061 gatggagcgc cagatggatg cggttgcaag gctcaaggca agaggcctga tcgtcaaggt
     6121 gaacacagtg gtcatccccg gtgtgaacga catccattcc gcggccgttg ccgagaggat
     6181 cgtgcgcctt aaagccgatc tgcacaatct gattgccctg atccctgttc ctggaactcc
     6241 ttttcagggt accgccccgc catccccgga gcgaatgact cttcttcgtc gtgccgcgga
     6301 agcttttatc ccgcagatgc gccactgcgt tcggtgccga tccgacgccg tggggcttct
     6361 tcatgacgac aggtcctgca tgggagctca cccggccctc gaaccccagg ctgccgggac
     6421 ctcggccggg agtggaactt gttcggtaga cctccgagtg gtgggaggaa acggaagcct
     6481 tcgagatgtg cataggcttt ccatgcctta gcagatgcga acattcagac gcaactgaag
     6541 tgaaggcgca gctcgcaagg cgtaatcctt tcccgtcccg ggacacacga aacagagctt
     6601 catcgtatcg aggttcctca attgagagtc gccattctag aaagaaggcc catcgccgta
     6661 aaattgaaat tcgaaatact ttagccgcaa gttggtagga tatagcaatc aatatagttc
     6721 gatatgcatc aatatgtcta tgtcgtttca gaggaacaaa tccttccatc ctccagccaa
     6781 tcaagcggcc tttcctctag gatcgaagcg tcgccatgag cctcaatgga tggagcaaca
     6841 gatccagtgc atccaggatc ccggaggtca cgacatcggc tgccaggacg gcagtcacgg
     6901 cggctccctc cgtttgcacc accgctatcc ccaataccgc ttcccgaagc atccgttcgt
     6961 cattgcggcc gttgcccaca caaacgcaac tgttcggacc caggcgctcg acgtagcgga
     7021 gcttcgcttg cgcctgctct tggcgaggga tgatcgacag ccgacaaggg atcccggcaa
     7081 gtgcctcatg aacgctccca aaggtgtccg ccgtgaggat gtgaatctca agcccggctg
     7141 agagtgtctc caggcgttct ctgaccccgg aaatgagccg cccgtcgcag gcaacggttc
     7201 cgttgtaatc cagaaccaga tgggaaagat tcatgggccg gtcatctccc gggatatgga
     7261 tttcaagcat acttcaatct ccttcctcat gctcagaagg catcttcaat gagcttctcg
     7321 aatgtcttgg gatcttgcag gcactctccg gaaatttcct gtccgataac tccttcgcgc
     7381 aggacataga ccttgtcggc caggcgtcga gtctggccga ggctgtggga aacagccaaa
     7441 acggtgtaac gttgtttcag gctggtcaag agttgctcga tcttccgagc ggccctgaag
     7501 tcaagggatg ccgtaggttc gtccaacagg aggatttgag gttccagggc cagtgcgcgg
     7561 gcaaggcaga ggcgttgctg ctggccgcct gaaagggtcg tggcggcgtc atcgagcctg
     7621 tctttcactt cctcccaaag ggaggcttcg cgcagtgccc gttcgatgcg ctcggccagc
     7681 acactcccgg cgatgcccag ggtggtcttg agcggcatgg cgagattttt tctgatggaa
     7741 aaagggagca caccgggggt ctggaaaacc attccggcct ttctgcgaag ttccggaagg
     7801 gggagaaaat cgtcgtaaac gtttccccat cggccatcga gccggatccg cacttctccc
     7861 cgagtccggc agcccgggaa gcattcgttc aaccgattga cggccctgag caaagtggat
     7921 ttgcccgatc ccgaaggccc cacgatgact gtcagttctc ccgggcaaag ggcgaggtcg
     7981 acggagttca agaccatctt tcccgagaaa gcaaccgaga gatctcgaat cgtaacggtc
     8041 gcgctgctcg aaggtgctag tgtgttttcc atcgcttctc aaattttgcg tgcaggaaaa
     8101 aggctgccag gaagagaaaa ccggtcagca gcaggagcac gagagccgcg ccgaatccct
     8161 gatcgagttc ggcttggctt ctatgctcgg ccgccaggta gtagatgcgg aagggaagcg
     8221 cctcgaactt gtcccaaagg ctggcgggaa ggccggcacg ggccacgact cccgtcagga
     8281 gaatcacggc cgtgtcttcc gcggcccgcc cgatggcgag aatcaccccg ctcagaatcc
     8341 cccggcctgc cagaggcacc aggaggtacc ggatgttatg ccaatggctg agcccaaggc
     8401 tcgggccgag cagccgcaga tgctccggga tcgcttcaag agccgtctgg gtcgttcgga
     8461 tgacataagg cagaatcagg agtgcaatac agaccgctgc gagaaagagg gaaggtcttg
     8521 cctgtggaag cagggtgttg cgaagaaaga ggatcatcgt gaatccgaag agccccatga
     8581 cgatggaggg agtgccggac aggacatcca cggcaaatcc caggaacgag cgtacccgtc
     8641 ctgaagcata ttcggaaagg tagacgccgc ttgcaacacc ggtcggaatg gagatggccg
     8701 aagagaggag gaccaggaaa agagtcccca caaccgcagg ccatatgcct tcaaataccg
     8761 gctgtgtccc ggtgatggcc gaaagccaat gggtgtcgcc gaaaaagagc ttcaagtcga
     8821 cggagctcaa tccccgtttg atcaagaaac ccaggaggag cgaggtggcc aggaagaccg
     8881 caacggcaca gagccgggcg tagccggtca tgagaaaatc aaggttcttc ttcatgccgg
     8941 tcctttccta tcccgtcatc cagccttcga atgaggaaat tggctccgcc catcagcaga
     9001 aagaggatga gacccgaggc gaagatggac cgataggaca ggctctggct gtctgttgcc
     9061 acaaccaggg cgacgtgggc cgtgagcgtt cggacggaat caaagaggga gcttgggatt
     9121 tgtggggcgt ttccggcgac catgagcgaa atcagggtgt ccccgacggc ccttccgaat
     9181 cccatcaccg cagccacaat aaggcctcgc gaggatgcag ggatgagtac gtgaaggaat
     9241 tgccgtgcgg gagtgagccc catggcttcg gcggtgagcc gaagggaagg ggcgatctgt
     9301 tgaagccggg catggaagat gaggacgatg gtcggcagga tgagcaggct gagtgtgagc
     9361 ccggccgtca gaaaggagaa gcccgtaccg atcccgagcc ggtccctcac caggggaacg
     9421 agcaggaaga cggaaacgaa gccgtagatg accgtcggga tgctcgtcat gaaatggatc
     9481 aaaatcagaa agaaacgagc aagacccctc ggggcaagaa cggtgacaaa accgcagatt
     9541 ccgatggctg cgggaaatgc caggccgaga gcaaacaccg aaaggagaag ggatacaagg
     9601 cacatgggaa ggataccgaa atggccggaa aaaggtttcc actcccaaga aagaactgcg
     9661 ctcatcccgt ccgaggtgat gagcggcagg cagaaataga cgagcagggc gagcataaaa
     9721 agaatcgcca cggcggaaat tcctgtgacg atcaagaggc tccgttgaat ccctctttcg
     9781 gagatcaggc cggcacgatt cgcccccatc cctgtcccgc tgtttccata caagcgattc
     9841 atactactcc agggggatgt agccgtgcct cctgatgatt tccgaacctt cagggcccag
     9901 gatgtagtcg atgaatgcct tggtgagcct cgccggctct cccttggtgt tcatgtagag
     9961 ctttcgaacc acgggatagc ttccgttcga ggcgttctcc tgggtggcgg caacggactc
    10021 gatcttgacg gctttgaggc tctggtccac gtgtccgatc cccacatagc cgacggcgtc
    10081 cttgtcccgg gaaatcgcca tcttcatggc cccgttggag gaaacgatgt tggccgtgcc
    10141 tgcgacgggt ccctttttca gaagcttctc ccagaagacc tcgcgagtgc cgcttgcctc
    10201 atcgcggcag aaaaggtgaa tggaaccgtc cttcccgccg acgtccttcc agttggtaat
    10261 ggagcccgcg aagatatccc ggacctgctg ctgggagaga ttgtccacag ggttatcggg
    10321 atgaaccgcg acagcgactc catcgagggc gaagggaaag gatttcaagc cgtacttggc
    10381 gatttcctct tgggatacgg cccgaccggt gtttccaatg tccaccagac cctccccgac
    10441 cttctggact ccgacacccg aacctccacc ctcaacggtg atccggatct tcgggttgaa
    10501 ggtcatgatc gccttggctg catcgttcat gacggggatg tgtgccgtgc cgcctgcaat
    10561 gccaatcgtc ccttcgagcc ccgcaaaccc gtccagggct ccgcaaaggg cagcggaccg
    10621 aaaccaaccg agaagaaaaa agaccgaaag aacggcgacc acattcatgc gtttgcgcaa
    10681 aatacccatg ctgttcccct cccaatgaac ccttgtctgc cttgttggtt ttcatgcttc
    10741 cggtcctgcg cgtgccggcc ggatctcgcg tcacgtcatc cctgcggaat aaagactccc
    10801 attgaacggg gcgcaacccg ttgaatggac gattccccca cagccgggaa tcgatgatga
    10861 tcgtgcacac ccggtggatg cccgcctatg cggacatgaa gccctggtgc tcatggaatc
    10921 gccaaaatca aaaaacccct tcacatttcc cgggccgaag ttcccatggg atcgccgaag
    10981 ggcgggacag ggtgaggtca agctttgcac agacctcgga agatgatccg aaggtccctt
    11041 tcatcaatct cacgaaacag gatttcgtcc tcgagggcct caggttcttg tacgcgcggg
    11101 gattcccggg ggtccgggtc ctcgggggga acggacaggg tcgagcttct ccgcttcgaa
    11161 gggatgttct cgatgccggc attgtttcgg agccagtggt cgcattcgtc atggcttccc
    11221 aggaacacaa agaacaggtc ttccccctgg aggatgccga tgagccgata accccggacg
    11281 aggtcgtatt tcacacagtt cttgatcctt gcctctccgt accgggtcat tctgccgacc
    11341 tggttaagat ttcgacgcac atccgagagt aactggtcga tgatgttccg ggcatggacg
    11401 gcggcggcgg cgcctcgtcc ccctgccttc cggaggacct tgagctgttt cttgagggtt
    11461 ggatccaggt gtatgttgcg aatcatgtca ctcctcgacg gcctcggtcg gcccccccgc
    11521 aagaccgcac ttgcaaatga tcccgggcaa atccccctgc gtgggcattc atcctccccg
    11581 gtcggctccg cacccacgcc tcgcgcacag accgctcgca gaaattgcat gcggccttga
    11641 ccttgcgcct ccgttcatcc cgtgtcgctt acggaagaag tgcagggacc cgggaagggg
    11701 caatgaagca cgaaacccat gccgcgggct tcccggccga gcgaacaaac cgccccgagg
    11761 atcggttacc actcggaaac gtttctgtct aatcggtttt ttgcgggtct tggccggtgc
    11821 ttcatcggaa acggcaatga atccaaagga tcggagaccc attcacggtg gaccggggac
    11881 ccggcgccgt ggggcacggt gccgggtcaa aaccccccga ggcgcttggg gattctcctt
    11941 ggccgtgata tgcttcgatt ccgccgttga gaatggatgt tggggaagga tacatccccc
    12001 ttggggctct cgtttcgtgg acgtctcggt gctctgtggg gatctgtcct cccatggggt
    12061 gaaatctcga aggttggatt ctcgtcgtgg acggcacgat ttgctgttcc gtgcccaagg
    12121 aggtacctcg tggccgggag cgccgtcagg gggaagcggc ggggaaggag cctcactcaa
    12181 agaaggactg tgtcgggcat gatgaaaggt cggttttctg gaagaaggac ccgaaatttc
    12241 caggttgtcc tggtcggaat ggcagtgagc ctgcttctga gcggatgcgg cgagccgaac
    12301 ccccggctgt accgtgtggg gattcttagc ggcctggata cgtttgccgc cacggtggac
    12361 ggtttcaagg ccaagatgac cgaactgggg tatatcgagg gcaagactat tgcatatgaa
    12421 acggaaagga ccaatttcga ccaggaggcg gaggagcgga tccttcgaag atttgtcgac
    12481 gaaagggtgg acttgatcct cgtattccct tcggaggcat cggtcgctgc caaggagatc
    12541 acacggggaa cctcgatccc ggtagtgttt tgccagacca acatcgaggg tacggacctg
    12601 gtccgaagtg tgttggaacc gggggagaat ctcaccggtg tacgctttcc cggaccggac
    12661 atcgccgtca agcgcctgga ggttctcctt gagctcgcac cccgggccaa acggatctgg
    12721 gtgccctatg ggaggaatgt ccccatcgtg cccgatcagc tgacggctct tcgtttggag
    12781 gcggcaagag gcggcattga cctggtggaa gctcccgctg acggcgccga cgatttgttg
    12841 gcggatctgg aaatgaggga aaagacggcc gatccgggga tcgacgcgat cctgctcatc
    12901 tccgaacccc tggcgaagac ccccacggtc tttcccacca tgatccgctt tgcgtacaga
    12961 caccggatcc ccgtcggggg attcgctatc tcccacgaag gccattcaac ggtgtttggt
    13021 gtggccacgg acaatcttgc cgtaggcagg actgcggcca tccaggcaca caaggttctg
    13081 agggggattc cggccggcac catcccggtg gtttcggctg agagttattt ccacatcaac
    13141 tacagggcgg cgcaagaact gggcctgagt gtcccggaag ggttgttgcg acaggcaaac
    13201 gagatcgtcc gctagtcgga aatccattgc acttgcggct ccggagagcc gcaaaggtgt
    13261 cggagaagag ccatgaagac ccgggtggag actttttctt cagggcggaa actgagcaca
    13321 accttggcgg gggccttctt cctcttcagt gcggtcgtac tcctcctcta caccggcttg
    13381 atgggacttt atgattacag gaccttggaa gcgctcattt caagccggca gcagctgatt
    13441 gcccgagatg cagccaaggc cgtgagcggc ttcatccacg ataacttcag cgttctggaa
    13501 acggcgatct ggttgaccga ttttgacacc cagcgggagg tggaacggag gcggattctc
    13561 cagagtttgc tgggactgcg tcccgctatc aggagactcg tgctgttgga cccccacgat
    13621 caggtcctgg cccgggcgtc tcggctctcg atgcaggcat ccggccaatt cacggaccgc
    13681 cgtctcgagg aactcctctc ggggcaggat cgaaaggcca agagacgaat gagcacggtc
    13741 accattgatc ccgtgaccgg cgaacccctc gtctggctgg cggtcccggt taccgacgcc
    13801 ttcgaagacg tcaggggaac actcatcgcc gagctcaatt tgaagtccat gtgggatatc
    13861 gtggaccaac tccgggtggg cgagaccgga tacgtctatg tggccgacag ggaaggcaac
    13921 ctgctcgcgt tccaggatac ctcccgggtg ctcaaggggg gaagagtgtc tcatatccgg
    13981 gtggttgccg acttcatccg gaataagccc gccgtgcaac ctgttgcggt agagcgattt
    14041 cagggaatca agggatccac ggtggtggga acctatgctc ccttgaccat gccggattgg
    14101 gccgttgtca ccgaactgcc gtgggaagag gcgtaccggg tgatggcgcg caatctggcc
    14161 gcggccgtcg ccttcacact ggggatcgcg attctggcag ggatcttcgg agtgcgggtg
    14221 gcaaggcgta tggcgaagcc cgtcatccag ctgacggaga cggcctcccg gattgctgca
    14281 ggggagccgg acctcaaggc ggaggtgggc ggaccccggg aagtggcttg cctggctgtc
    14341 gcattcaaca gcatgacgtc tcaactgcgg cagagcctcg tggatctcga ggatcgcttt
    14401 ggcgaattga agcgcacgga agaggcttta cgcctcagcg aacaacggct tcgcctggcc
    14461 ctcgaagcga ccagcgacgg gatttgggac tggaatctcg agaccggccg ggtgtatttc
    14521 agcccccgct attacaccat gatggggtac gaaccggacg aattcccggc ctcttatgaa
    14581 aactggaagc gatgggtcca cccggtggat ttggaggcgg ctgagaaatt ggtttggcag
    14641 gcaatccaag accgcacctc gtacaccatc gagtttcgct tcagggcgaa ggatggagac
    14701 tggcgatgga ttctggctcg gggcaaggtg gtggaaacgg atgaggccgg aaaggccgtt
    14761 cgggtgtccg gaacccattc ggacatcacc ccgcgcaaac gggtggaaga ggcactcgag
    14821 aagcgcattt tggcgttgac ccagcctctg ggttccgtcg aaaacatcgc cctcgaggac
    14881 atcttgaatc tttccgatct ccagcatttg tcggatctgt tcgccaaggc cttcggagtt
    14941 gcatccctca tcacccggcc ggacgggacc cccatcaccc agcccagcaa cttcacttcc
    15001 ttttgcagtg aaatcgtccg caaaactccc cggggactcg aaaagtgcaa ccattccgac
    15061 gcgatcatcg gacggcacaa tccgtccggc cccaatatcc aaccttgctt gagtgccggg
    15121 ttgtgcaacg ccggggccag cattacgctc ggcggatccc atgtggccaa ttggctgatc
    15181 ggtcaggtca gaaacgaggg cgtggacgaa gaaaaaatca tggaatacgg tcgggagatc
    15241 ggtgcggacg aaggggtctt ccgcgccgca tacctcgagg tgcccgtcat gtcccaagag
    15301 caattcgaca atacagccca tgtccttttc gccgttgcaa accagatctc caacagcgcc
    15361 tatcaaaaca tcctccaggc gcggttcatt tccgaacgaa aacaggcgga ggagagtctt
    15421 cgcaagtacg agcgcatcgt ttcctcttcc caggacgtga tggccctcat caacaccgat
    15481 tacgtctatg aaaccgtcaa cgaaagcctc ttgaagttgc agggacggtc ccgcgaagag
    15541 attgtgggcc ggacggtttc caaggtgatg ggggagaaga ctttccggca taagctgaag
    15601 gcgcgcctgg accaagccct ttccgggcaa acggtccact ttcaggaatc cttcgactac
    15661 ggagaaacgg gtcgacgagt catggatgtc aattactgaa cttttgcaga atgcatgtaa
    15721 gtgactgaaa aggtttgaaa tgattggg
//
LOCUS       DAOOVC010000092        15589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2529_length_15589_cov_6.907558, whole genome shotgun sequence.
ACCESSION   DAOOVC010000092 DAOOVC010000000
VERSION     DAOOVC010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15589
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2529_length_15589_cov_6.907558"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..186)
                     /locus_tag="PLM79_11835"
     CDS             complement(19..186)
                     /locus_tag="PLM79_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87046.1"
                     /translation="MALVVQKYGGTSVGNPDRIRSVARRVVDRKKAGDHLVVVLSARA
                     GDTDRLSAWPL"
     gene            502..2037
                     /locus_tag="PLM79_11840"
     CDS             502..2037
                     /locus_tag="PLM79_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HOV87047.1"
                     /translation="MKILFVYPRVAETFWSFKHALRVVARKSAFPPLGALTVAAMLPS
                     DWTKRLVDMNVKELSDQDLLWADYVFISAMIAQKESTHQVIRRCRSLGVKIVGGGPLF
                     NAYKEDFSHLDHLVLGEGEITVPQFVQDLRAGCPKSRYESQDHPPLHHTPIPLWELID
                     LNDYVSMSVQYSRGCPFNCEFCDIIVMNGRIPRTKSDDQMIAELDALYRRGWRGSIFI
                     VDDNFIGNRKKVKRLLRRIIQWQADRRGRLNFFTEASVDLAEDEELMDLMVAAGFNKV
                     FLGLETPSEESLKECGKAQNLMRNLSESVRRIQSRGLAVMGGFIIGFDSDPPDIFQRQ
                     VQFVQRNGIVTAMIGLLTAIPGTRLYRRLEEEGRLLFKASGNNTDTSGSLNFVPRMDR
                     NIIIGGYRWVMNTLYSPEMYYRRILAFLNQHKPVVKTHLESNDLFTFLRSLWYLGVED
                     QKNGHNYYWSLVKEGLTRYRKSFADIVTLSIYGYHFRRLFWSPYLPVNWEWWKGHVLS
                     ADP"
     gene            2273..2611
                     /locus_tag="PLM79_11845"
     CDS             2273..2611
                     /locus_tag="PLM79_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HOV87048.1"
                     /translation="MMEEMKISRKDIGIRLLFTILFLIVFEVLKLVVQIVVLFQFVCL
                     LITRKFSSPLRVFSNKVATYAYRVIRYVTLNENTRPFPFRELPEPMEEAEEPVVFGCS
                     ECKGAGETRD"
     gene            complement(2767..3282)
                     /gene="tsaE"
                     /locus_tag="PLM79_11850"
     CDS             complement(2767..3282)
                     /gene="tsaE"
                     /locus_tag="PLM79_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOV87049.1"
                     /translation="MQELTFRSHDEERTLALGSFLGSRLEPGDVLALWGELGAGKTLF
                     ARGIAWGLEVPREVPITSPTFAFIHEYEGRLHLYHMDLYRLSHPDELDTLPWKEALYG
                     RGAAVVEWPDRLGADLPPERWDIEIRVTGDASREILLRAVGKRNGARLELWAPDLFDL
                     RSESGEPCRRG"
     gene            complement(3270..4844)
                     /locus_tag="PLM79_11855"
     CDS             complement(3270..4844)
                     /locus_tag="PLM79_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOV87050.1"
                     /translation="MRIATASEMAQLDRRTIEEVGIPGIVLMENAARGAAGFFLEVFP
                     DLLRKRITVVAGSGNNAGDGFVLARIFQNSGGQVRVVCLRPPDGLQGDALTNYRILEN
                     MGTTIHIWDEEGDFSSQWRWIEESDVIIDAVLGTGLRNEVRGTYRKVIEGINDLGVPV
                     LAVDIPSGLHAGTGKPMGAAIRARATATFGLIKLGHLLEPGTAYVGRLSVVDIGIPVH
                     IVESADIRRWWLDHELLSTWIAPRPPAAHKGNAGHAVVLAGSPGKTGAAALACLGAGR
                     VGAGLVTLLIPSSLNPILEVKLTEAMTVPIPETEDHSPSEAALPLILDFLQDKQALAM
                     GPGISLHSSTQTLVKNLLVQSPCPMVLDADAITAMAPEPERFKDAPHPVVLTPHPGEM
                     SRLTGKSTREIQENRVETAGEFAVKYGVTLVLKGHRSLIASPDGRLAVNSTGNPGMAC
                     GGMGDVLTGMIAGFLAQGFPAFEAACLGVYIHGAAADRRMGEISSRGLLASDLLEEIP
                     GVIGRLEGFEAPSCKS"
     gene            complement(5017..5397)
                     /gene="acpS"
                     /locus_tag="PLM79_11860"
     CDS             complement(5017..5397)
                     /gene="acpS"
                     /locus_tag="PLM79_11860"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOV87051.1"
                     /translation="MGVYGVGIDLVRVERLEKSLEKWGARFRERVFTAHEIEHCSRRK
                     NSVSCLALRFAAKEAFAKALGTGIRAPLSWLDIEVRNHAGGKPEVFLSSRAARYCRDR
                     GILAWHLSLTDDGAYGAAVVVLES"
     gene            complement(5399..6130)
                     /locus_tag="PLM79_11865"
     CDS             complement(5399..6130)
                     /locus_tag="PLM79_11865"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOV87052.1"
                     /translation="MKGGSVAKVSINVDHVATVRQARLAAEPDPVMAAALAELAGAQG
                     IVVHLREDRRHVQDRDVEVLRRTVKTKLNLEMAATPAMIQTALRIKPDLVTLVPEKRQ
                     ELTTEGGLDVVLYEESLKEVIKTLHDAEIAVSLFINPDPKHIKTAHKLETDYVEIHTG
                     SFADAKTFAQRKEEFDRVLTAARLAHKLGLGVHGGHGVDYRNILWLRSITEIEEFSIG
                     HSIIARAVLVGMEKAVREMIELVNG"
     gene            complement(6165..6560)
                     /gene="ybeY"
                     /locus_tag="PLM79_11870"
     CDS             complement(6165..6560)
                     /gene="ybeY"
                     /locus_tag="PLM79_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOV87053.1"
                     /translation="MGYTEAELSVAIVDDAQMRELNRKYRGVDSTTDVLSFSMTEGEF
                     GDVCPELLGDVVISAPTARILAEEYSCPLSTVLDLLLVHGILHLVGHDHGREEEETER
                     MFETTVELMKLLGHSEETFSWYLRTDSEE"
     gene            complement(6547..9024)
                     /locus_tag="PLM79_11875"
     CDS             complement(6547..9024)
                     /locus_tag="PLM79_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOV87054.1"
                     /translation="MEKEKRDSDKTKTKPKRAPATRYLPDFVQNLRHRNTAILLGLSF
                     ILACLLTPSFVTTSPKYQLGDIADRNTKAARDLLILDEAATAKKKEEALRQAPMVYDL
                     DETVAHRIQESVERTFQSMRDFLHHPPAPPPVPDVSLDGGGDVPPAYDNGSSIDLPAL
                     AQVKKEFQSTLGIQVPDEIFFELLERRWSRDIQDKVTQLLVSLLDRGILTREAIQVQS
                     RNQNLLLRKMGSQEEFLFTPPHSYLDIEDARKLARMRPYEDGWDSKEMRVVAYLASHL
                     LQPNLTFNLQETERRRELASSAVKPVYIQVAKNEMLVREGQRIGPEELLKLKANDQKA
                     SRGHRAVIFIGLLLFATVCIWVVVHVTREHLPARRMETRDYLFLAILLVFALALGAAA
                     QWMAELSAESSTHFGSKTLLYAVPLTTGAMMACIFFGVTVSLLFSLILTLFASMLFGR
                     DFGLFFYFLIGSFVAAHCTSSCRTRMVPIKAGLLVGGANVVLILLSALVLDQWVILRV
                     LTNAFFGLCGGIFAGILVTGFTPLVEMSFGYTTDIKLLELATMDQPLLQELMVQAPGT
                     YHHSIIVGNMVEAAAKSIGANSLMAKVAAYYHDIGKIKKPIYFIENQFDCENRHEKLA
                     PSMSSLILISHVKDGVELGRQHRLGKAILDIISQHHGTSFISFFYKKAQEAREKAKAG
                     RGTELPPINMDDYRYPGPKPQTKEAGLVMLADVVEAACRSLTEPTPARIQGMVNRLIN
                     NIFSDGQLEDCELTLKDLHEIARRFNQILATIHHKRIEYPATAAGEAKGKTDAPDSGQ
                     RESKSDRDRNAAGREISRADLKRLGIH"
     gene            complement(9037..10020)
                     /locus_tag="PLM79_11880"
     CDS             complement(9037..10020)
                     /locus_tag="PLM79_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOV87055.1"
                     /translation="MQASSVQLSFEDNRLVQHLAGDQNRHLKTMEKQLQIQVHLRGNQ
                     FTLVGDPPQVELAHRLIQELADLIQRGYPLYGSDILYAVGILKENIHRKLRDIFLDQV
                     FIASNKRTITPKSLKQKEYIEAIRHYDIVFGIGPAGTGKTYLAMAMAVAAMTKEQVRR
                     IVLVRPAVEAGEKLGFLPGDLAEKVNPYLRPLYDALHDMMDFEKASALLQRGAIEVAP
                     LAFMRGRTLNDSFVILDEAQNTTTEQMKMFLTRLGHNSKAVITGDITQIDLPENKPSG
                     LVEAIQLLKEIQGIQFVFFTNRDVVRHRLVQDIIQAYERAEHRRADAVNLP"
     gene            complement(10158..10976)
                     /locus_tag="PLM79_11885"
     CDS             complement(10158..10976)
                     /locus_tag="PLM79_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOV87056.1"
                     /translation="MIEVLQFEFMQNALLAGLLAGIACGLIGTLVVVNRIVFISGGIA
                     HAAYGGVGMAFFFGFSPLLGAAGFSLMISFLMALVTIRNKQRADTIIGVLWAVGMALG
                     IILIDLTSGYNVDLMSYLFGSILTVPTSDLLLMGAVDLAVIGLVSFFYNDFLALSFDE
                     EFSLAVGVPVKGLYFLLLAMMALSVVMVIRVVGLILVIALLTIPPYIAERYCGSLRSM
                     MVFSVVLNCLFTLTGLGISYAYNLTSGATIILVAAVCFFLVLLGDRLRRGRSRA"
     gene            complement(10978..11745)
                     /locus_tag="PLM79_11890"
     CDS             complement(10978..11745)
                     /locus_tag="PLM79_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOV87057.1"
                     /translation="MIAGAFAIEVDDLWFSYDGNLVLRGVNLRIEHGDFLAILGPNGS
                     GKTTFLKLLLGILTPSRGTIRIYGKEPGKIADRIGYVPQDTSVNKGFPISVLDVTLMG
                     RLGQPGRSRRYSTADRSVAQSSLERVGMWEYRDRPIGKLSGGQRQRVYIARALTAQPD
                     ILFMDEPTASTDKEFQTELYDFLKELNESMTVVVVSHDLSVLSSYIKSVACLSQTLYH
                     HDAAEITSEMIEKAYHCPVELIAHGLPHRVLRKHEDH"
     gene            12203..13324
                     /gene="mutY"
                     /locus_tag="PLM79_11895"
     CDS             12203..13324
                     /gene="mutY"
                     /locus_tag="PLM79_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOV87058.1"
                     /translation="MPNDLKKTYPRIRESMLRWFRDNRRDLPWRRSYDPYRVWISEIM
                     LQQTRVQTVLPYYERWMERFPHVRSVALAPEEDLLRCWEGLGYYSRARNVHRTSKILV
                     EAYGGEFPRDYDSVRRLPGIGAYTAGALMSIAFNEDFPVVDGNVKRVFHRVFNLGNGR
                     GAHESDRTYWALAAELLPRGQARDFNQALMELGALVCLPSNPSCPRCPLEGFCASLGA
                     GAVHEKPPRTRKKTSAIEVSVGLLFRGEEVFIQKRPPEGLMPNLWEFPGGKIRDDETP
                     EEALVREFREELNLGVRCLDPVAVIRHAYTSFRVILHAYRCALREGGQEPVLRAAVEA
                     RWVGLPQLDDYAFPAANRKLIEILVGRRHPDGRSRGKEA"
     gene            13326..14498
                     /locus_tag="PLM79_11900"
     CDS             13326..14498
                     /locus_tag="PLM79_11900"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOV87059.1"
                     /translation="MSNLSSLAGFVVAGMLLSLLLGCAGRDLRPGQAETAGGPIRPGD
                     IVEARSGMRISAESLSDRLAQVQIVYGGETHTSPADHRVQLEILRSLHAKNPRLVLAM
                     EMFPREVQPVLDRFSRGLIALEDFPGEVGWETVWGFPFPLYRDLLEFAQHHGLQVLGL
                     NAPHAVVQKVARSGLASLSPEERARIAREFRPENPRHREYVRKEFDDHVKETIKDFQT
                     FYEAQLTWEETMAETLADHLRSAPPGEQVVVIVGRGHLHYKLGIPRLVAERLAHSFKT
                     VIPLPVDHAEGSSDRELGDYYWITEKTEPAHRGRLGILIRPDSSGEGVEVVSVIAESP
                     AAKAGFRKGDIIVGVNDVVVKTPQDLHRAMAHKGKIHAVSVKRNGGEETMVVDLSP"
     gene            14684..14866
                     /locus_tag="PLM79_11905"
     CDS             14684..14866
                     /locus_tag="PLM79_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87060.1"
                     /translation="MATVMPEGEAIRKAIKWISEELKEHPEKTPTQLVGEATLRFDLS
                     PKEGDFLLGFYSKASE"
     gene            15053..15128
                     /locus_tag="PLM79_11910"
     tRNA            15053..15128
                     /locus_tag="PLM79_11910"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15086..15088,aa:Ala,seq:cgc)
BASE COUNT         3154 a         4602 c         4738 g         3095 t
ORIGIN      
        1 ggtccgggga agggctagtc acaagggcca ggcggataac cggtccgtgt cccccgcccg
       61 agccgacagg accaccacca ggtgatcgcc cgcctttttt cgatccacaa cgcgccgggc
      121 cacggaacga atccggtcgg gattgccgac cgatgtcccc ccatatttct gtactaccag
      181 agccataata cccgagacct ccctatccat ccgggtcctg tgacccaact ttttgtggtt
      241 tctttcgctt gcgaatctac ggccggatag aggcgcggag agaagagcgg aaagagcaaa
      301 cgactcctcg taacacaaat ggacttcaat gtgtataggt tttttggcga ggatccgtgg
      361 gagggtccaa tgcctgaaag gcccttggga tcatccccgg gaggggaaac ggaccttctt
      421 gctttctggg tggttgggcg gtacactgag atccatcatg gcgctgacca tccaccttcc
      481 agcattcggt gaggggcttt tatgaagatc ttgttcgtgt atccgcgagt ggccgagacg
      541 ttttggagct tcaagcatgc cttgcgggtg gtggcacgca aatcggcatt tcctcccctg
      601 ggggccctca ccgtcgccgc catgcttccg tccgactgga cgaagcggct ggtggacatg
      661 aacgtcaagg aactgagcga ccaggacctt ctctgggccg actacgtatt catcagtgcc
      721 atgattgccc agaaagaatc cacccaccag gtgatccggc ggtgccgcag cctgggcgtg
      781 aagatcgtcg gcggaggacc cctcttcaac gcctataagg aggatttctc tcacctggat
      841 cacctggtcc tgggggaagg ggaaatcacc gttccccagt tcgtgcaaga tctgcgggca
      901 ggatgtccca aatcccgcta cgagtcccag gatcaccccc ccctccacca cacgcccatt
      961 cccctatggg agctcatcga cctgaacgac tatgtaagca tgagcgtcca gtattcccgg
     1021 ggttgccctt ttaactgcga attttgcgac atcattgtca tgaacggccg tattccccga
     1081 accaagagcg acgaccagat gatcgccgag ctggatgccc tctaccgaag ggggtggaga
     1141 gggtccatct tcattgtaga cgacaacttc atcgggaaca gaaagaaggt aaaacgcctt
     1201 ctcaggagaa tcattcagtg gcaggcggac cgcaggggcc gcctcaactt cttcaccgag
     1261 gcgtccgtgg acctggccga agacgaagaa ctcatggacc tgatggttgc tgctgggttc
     1321 aacaaggttt tcctgggcct cgagacgccc tcggaagaaa gcttgaagga gtgcggaaaa
     1381 gcccagaact tgatgaggaa cctgtcggaa tcggtacgaa ggatccagag ccggggtctc
     1441 gcggtcatgg gcgggttcat catcggcttc gacagcgacc cgccggacat cttccaaaga
     1501 caggtccagt tcgttcaacg aaacggcatc gtcacggcca tgatcggtct tctcacggcg
     1561 attcccggca cacgcctcta ccgccgcctg gaggaagaag gccgcttgct gttcaaagct
     1621 tccggaaaca atacggacac ctccggcagc ctgaactttg ttccccggat ggatcgaaac
     1681 atcatcatcg gcggctaccg gtgggtgatg aacaccctct attcccccga gatgtactac
     1741 cggcgtatcc tggcatttct gaaccagcat aaaccggtgg taaagaccca tctcgagagc
     1801 aacgatcttt tcaccttcct gcgttctctt tggtacctgg gagtcgagga tcagaagaac
     1861 ggccacaact actactggag cctggtcaag gagggcctca cccggtaccg gaagtccttt
     1921 gccgatattg tgaccctgtc catttacggg taccattttc gcaggctctt ctggtccccg
     1981 tacctgcccg tgaactggga atggtggaaa gggcacgtgc tgtccgccga cccctgagag
     2041 cgtcctcgaa aaagtcccgt ttgcccttcg ggccgaaaca cggcgaaaca aagaatgccc
     2101 ggccccggtc ttttatcgaa gccggcattt ctcgtgttcg gctcgaagaa ttcgttttct
     2161 ccgggggccg agtggaaatg ggggtgagga aaaagagtgg cagacaccgg gattttggat
     2221 tgactttcat gggggaaaag aaggaacgct accgctaaaa aggaggttat gcatgatgga
     2281 agaaatgaag atatcgcgaa aggacatcgg gatacgcttg ctgtttacga tcttgtttct
     2341 cattgtcttc gaggtcctga agctggttgt gcagatcgtg gtcctctttc agttcgtctg
     2401 cttgctgatt acccgcaaat tctcttcgcc gctccgtgtc ttctccaaca aagtggccac
     2461 ctatgcctat cgggtcatcc gatatgtgac cctcaacgaa aacacgcgac ccttcccctt
     2521 ccgggagctt cccgagccca tggaagaagc ggaggaaccg gtggtgttcg ggtgttccga
     2581 gtgcaaaggg gccggggaga cccgcgattg atcccccgaa atggcggggt gttgtggttg
     2641 aattgagtgg tgtggttccc cccctcgagg gggggattcc cggaccggga gttgactgat
     2701 tcaacaggat ttccgggaag gagtgcgggg ggaaaggtcg gacgcccgga gacggcggaa
     2761 agacgctcat ccccggcggc aaggctcccc ggattcgctc cgcaggtcga agaggtccgg
     2821 cgcccaaagc tccagccggg caccgtttct ttttcccacg gcccgtagga gaatctcccg
     2881 gctcgcatct cccgtaaccc ggatctcgat atcccagcgt tcagggggca agtccgctcc
     2941 gagtcggtcg ggccattcca ccacagccgc tccccggccg tagagggcct ctttccaggg
     3001 cagggtgtcc aattcgtccg gatggctcag ccggtacaga tccatgtggt aaaggtgcaa
     3061 gcggccttcg tattcatgga tgaaggcaaa ggtggggctg gtgatgggga cctcccgggg
     3121 tacttcgagc ccccatgcga ttccccgggc aaaaagggtc ttccccgcgc ccagttctcc
     3181 ccaaagggcc aatacatcgc cgggttccag ccgggatccc aggaaacttc ccagggcaag
     3241 ggtgcgctct tcgtcgtggg agcggaatgt cagctcttgc atgagggcgc ctcgaagcct
     3301 tccaggcggc cgatcactcc ggggatttcc tccaaaagat ccgaagccaa aagcccccgg
     3361 ctcgagatct cccccattcg gcggtcggcc gcagccccgt gaatatagac tcccaggcac
     3421 gcggcctcga aggccggaaa gccctgggcc aggaagcctg cgatcatgcc cgtgaggacg
     3481 tcgcccatcc ctccgcaggc cattccgggg tttcccgtgc tgttgacggc aagcctgccg
     3541 tccggggaag cgatgaggga ccgatggccc ttgagaacga gggtcacccc gtatttcacg
     3601 gcgaactccc cggccgtctc cactcgattc tcctggattt cccgggtgga ttttccggta
     3661 aggcgggaca tctctccggg gtgaggagtc aacaccacgg gatgcggcgc atccttgaat
     3721 cgttcgggtt ccggggccat ggccgtgatg gcgtcggcat cgagcaccat gggacagggc
     3781 gattgaacca ggaggttctt gacgagggtc tgggtgctgg aatggagaga tatccctggg
     3841 cccatggcca atgcctgttt gtcttgaaga aaatccagga tgagaggcag tgcggcctca
     3901 ctgggggaat gatcctcggt ctccgggatg gggaccgtca tggcttcggt caatttcacc
     3961 tcgagaatgg gattgaggct tgacggaatc aggagggtta ccaggcctgc gcccacccga
     4021 ccggctccca ggcaggcaag ggccgccgcc cccgtttttc ccggagaccc ggccaacacc
     4081 acggcgtgcc ccgcgtttcc cttgtgagcg gcggggggcc gcggagcaat ccaggtactc
     4141 agcagctcgt ggtccagcca ccatcgccgg atgtcggcgg actcgacaat gtgaacggga
     4201 atgccgatgt ccacgacgct gagcctcccc acgtaggccg tacccggctc caggaggtgc
     4261 cccagtttga tgagtccgaa ggtggccgtg gcccgggcgc gaatggccgc ccccatgggc
     4321 tttcccgtgc cggcatgaag ccccgaggga atgtccacgg caagcaccgg gactcccaga
     4381 tcgttgatcc cttcgatgac cttccggtag gtgccccgga cctcgttccg gagccccgta
     4441 cccaaaacgg cgtcgatgat gacgtcgctt tcctcgatcc atctccactg ggaggagaag
     4501 tctccctcct cgtcccaaat gtgaatggtc gtccccatgt tttcgagaat gcggtagttg
     4561 gtgagggcgt ctccctgaag accgtccgga gggcgcaggc acaccactcg aacttggccg
     4621 ccgctgtttt ggaagatcct cgccaagaca aagccgtctc cggcgttgtt gccgctcccg
     4681 gcaacaacgg tgatcctctt tcgaagcagg tcgggaaaga cctcgaggaa gaaccccgcc
     4741 gccccccggg ccgcgttttc catgagaacg attcccggga ttccaacctc ttcgatggtt
     4801 cggcggtcca gttgcgccat ttccgacgcc gttgcaatgc gcatatcggt ctccgcgtct
     4861 gttccccgtg ccgctcccgg gggggcactc tcccgaggga gcggtgttcc ggagggatcg
     4921 gggagagact ccgaggccgg aagtgcccgg tttcctcccc caatcctcgc taaaggtccg
     4981 ggaaaatggg tcgaatggat gggaccggcc ggggagctaa ctttcgagta ccaccactgc
     5041 agctccgtaa gccccgtcgt cggtgaggct caggtgccac gcaaggatgc cccggtcccg
     5101 gcaataccgg gcggcccggg aggagagaaa gacctcgggc ttgccccccg catggttgcg
     5161 aacctcgatg tccagccagg acagaggcgc ccggatcccc gttcccaggg ccttggcgaa
     5221 ggcctctttg gcggcaaacc gcaaggcaag gcaggaaacg gagtttttcc tgcgggagca
     5281 gtgctcgatc tcatgggcag taaacacccg ctcccggaaa cgggcccccc atttctccag
     5341 gctcttttcg agtctttcaa cgcgaaccag gtcgatgccg actccgtaga cgcccatctc
     5401 agccgttcac cagttcgatc atttcccgaa ccgccttctc catccccacc aaaaccgctc
     5461 ttgcgatgat cgaatgcccg atgctgaatt cctcgatttc ggtgatgctg cgcaaccaaa
     5521 ggatgtttcg gtaatcgact ccgtgtcctc cgtggacccc caggcccagt ttgtgagcca
     5581 gccgggcggc tgtcaagacc cggtcgaact cttcctttcg ttgggcaaag gtcttggcgt
     5641 cggcgaagct tcccgtgtga atctccacgt agtccgtttc cagcttgtga gcggtcttga
     5701 tgtgcttggg gtccgggttg atgaaaaggc tgacggcgat ttccgcgtca tgaagggtct
     5761 tgatgacctc cttgagggat tcttcataca acaccacgtc cagtccccct tcggtggtga
     5821 gctcctgcct cttttcggga accagggtca ccaggtccgg cttgatgcga agggcggtct
     5881 ggatcatggc gggcgtggcg gccatttcca ggttcagctt ggttttcacc gttcggcgca
     5941 aaacctccac atctcggtcc tggacgtggc gcctgtcctc gcgcaaatgc accacaatcc
     6001 cctgggcccc cgccaactcg gcgagagcgg ccgccatgac gggatcaggc tcggcggcca
     6061 accgtgcttg tcggaccgtc gccacatgat ccacgttgat cgaaaccttg gccacacttc
     6121 ctcctttcat ccaatgcttc cacgaggggc actccccgcg gggatcattc ctcggaatcc
     6181 gtccgcaaat accaggagaa ggtttcttcc gagtgtccca ggagcttcat gagctccacc
     6241 gtggtttcga acattcgttc cgtttcttcc tcttcccttc cgtggtcgtg gccgaccagg
     6301 tgcagaatcc cgtgaacgag cagcaggtcc agcaccgtcg agaggggaca ggagtactcc
     6361 tcagccagga tccgggccgt gggggcggaa atgaccacgt cccccaaaag ctccgggcac
     6421 acatccccga attctccttc ggtcatggaa aaggaaagga cgtcggtggt cgagtccacc
     6481 cctcgatatt tccggttgag ctccctcatc tgagcgtcgt ccacaatggc gacgctcaac
     6541 tccgcctcag tgtatcccaa ggcgtttaag gtccgctcgg ctgatttctc gaccggcggc
     6601 attacgatct cgatctgatt tggattcacg ctgacctgaa tcaggggcat cggtcttccc
     6661 tttggcctcg ccggccgcgg tggccgggta ttcgatccgc ttgtgatgga tcgtggcaag
     6721 gatctggttg aaacgccggg cgatttcgtg gaggtctttg agggtgagct cgcagtcctc
     6781 gagttgcccg tcactgaaaa tgttgttgat gagccggttc accattccct ggatcctggc
     6841 gggggtgggt tccgtcaggg agcggcacgc ggcttccacc acgtccgcca gcatcaccag
     6901 cccggcctct ttggtctgag gtttgggacc cgggtagcga tagtcgtcca tgttgatggg
     6961 aggcagttcc gttccccggc cggctttcgc cttctctcgg gcctcctggg cctttttata
     7021 gaagaacgag atgaagctgg taccgtgatg ctgactgatg atgtccagga tggctttgcc
     7081 caggcggtgc tgcctgccca gctcgacgcc gtctttcacg tgggaaatga gaatcaggct
     7141 gctcatggaa ggagccagct tttcgtggcg gttctcgcaa tcgaactggt tttcgatgaa
     7201 atagatgggc ttcttgatct ttccgatatc gtggtaatag gcggccacct tggccatgag
     7261 cgagttggcg ccgatggact tggccgccgc ctcgaccatg ttgccgacaa tgatgctgtg
     7321 atgataggtg cccggggcct ggaccatcaa ttcctggagg agcggctggt ccatggtggc
     7381 cagctccagc agcttgatgt ccgtggtgta cccgaagctc atttccacca agggagtgaa
     7441 accggtcacc aggatcccgg caaagatccc tccgcagagt ccgaaaaagg cattggtcag
     7501 cacccgcagg atcacccact ggtccaggac aagggcgctg aggaggatga gaacgacatt
     7561 ggcccccccc accagcaggc ccgccttgat gggcaccatc cgagtgcggc acgaggaggt
     7621 gcagtgggcg gccacgaaag agccgatgag aaaatagaaa aacagtccga agtctctgcc
     7681 gaaaagcatc gaggcaaaga gggtcaaaat cagggagaag agcagggaga ccgtgacccc
     7741 gaagaagatg caggccatca tggcccccgt ggtcagcggc acggcgtaaa gaagggtctt
     7801 cgacccgaag tgggtggaac tctccgccga caactcggcc atccattggg cggcggctcc
     7861 cagggccaga gcgaaaacca ggaggattgc cagaaaaaga taatcgcggg tctccatccg
     7921 gcgcgcggga aggtgctccc gggtcacgtg gaccaccacc cagatgcaga cggtggcaaa
     7981 aagaagcagc ccgatgaaga tcaccgcccg atgcccgcga gacgccttct ggtcattggc
     8041 tttcagtttc aagagctctt cgggcccgat tcgctgcccc tcccgcacga gcatctcgtt
     8101 cttggccacc tggatataga ccggttttac ggcgctggaa gccagctctc ttcgccgctc
     8161 ggtctcctgg aggttgaagg tgaggttcgg ttgcagcagg tgcgacgcca ggtaggccac
     8221 caccctcatt tccttggaat cccagccgtc ctcgtagggg cgcatgcggg ccagttttcg
     8281 cgcatcctcg atgtccaggt aggaatgggg aggggtgaag aggaactcct cctgggagcc
     8341 catctttctc aacaacaggt tctggttgcg gctttgcacc tggatggctt cccgcgtgag
     8401 gatgccccgg tccagaaggg agaccagcaa ctgggtcacc ttgtcctgga tgtctcggga
     8461 ccagcgcctc tcgaggagct cgaagaagat ttcgtccggc acctggatgc ccaaggtgct
     8521 ttggaactct ttcttgacct gggccagggc gggcaaatcg atcgaggaac cgttgtcgta
     8581 ggcgggcggg acgtcccccc cgccatccag ggaaacatcc ggaaccgggg gaggggcggg
     8641 cggatgatgc aggaaatccc gcatggattg aaaggtcctc tccacggatt cctggatcct
     8701 atgggccacc gtctcgtcca ggtcatacac catgggtgcc tggcgcaggg cctcttcctt
     8761 cttttttgcg gtggccgcct cgtccaggat gaggaggtcc cgggcggcct tggtgtttcg
     8821 gtcggcgatg tctcccagtt ggtacttggg actggtggtc acgaaagagg gtgtgagcag
     8881 gcaggccagg atgaagctca gtcccaggag gatggccgtg ttccgatgcc gcaggttctg
     8941 cacgaagtcg ggcagatacc gtgtggccgg ggcccgcttg ggcttggtct ttgtcttatc
     9001 cgagtcccgc ttctcctttt ccatgctttt ccgatcctaa gggaggttta ccgcatcggc
     9061 ccttcggtgc tctgcgcgct cgtaggcttg aatgatatct tgaacgagtc ggtggcgcac
     9121 cacgtcgcga ttggtgaaaa acacgaactg aattccctgg atctccttca acaattggat
     9181 ggcttccact aggcccgaag gcttgttttc gggaagatcg atctgggtga tgtccccggt
     9241 gatcaccgcc ttggaattat gtcccagccg ggtgagaaac attttcatct gttccgtggt
     9301 ggtgttttgg gcttcgtcca gaatgacgaa agaatcattg agggtgcgtc cccgcatgaa
     9361 cgccaaggga gccacctcga tggcgccccg ctggagaagg gcggacgcct tctcgaagtc
     9421 catcatgtcg tgaagggcgt cgtaaagcgg acgcaggtag gggtttacct tttcggccag
     9481 gtcccccggg agaaacccca gtttctcccc cgcttccacg gcgggccgca ccaggacgat
     9541 tcgtcgcacc tgctccttgg tcatggccgc caccgccatg gccatggcca ggtaggtttt
     9601 cccggtcccg gcagggccga tgccgaagac aatgtcgtag tggcggatgg cttcgatata
     9661 ttccttttgt ttcaggctct tgggcgtaat ggttcgcttg ttggaagcaa tgaacacctg
     9721 gtccagaaag atgtctctca actttcgatg gatattctcc ttgaggatcc cgacggcata
     9781 aaggatatcg cttccgtaca gggggtatcc gcgctggatc aggtccgcca gctcctggat
     9841 cagccggtgg gccagttcca cctggggcgg gtccccgacc agggtaaact gattgccccg
     9901 gagatgaacc tggatctgca actgcttttc catggtcttg agatggcggt tctgatcccc
     9961 cgccaaatgc tggaccaggc ggttgtcctc gaaggacagc tgcacgctgc ttgcctgcaa
    10021 agaattgttc gtggcagcct cgaccaatgc ctgttgccct caaccatcaa accaaagggg
    10081 ttttccgcga tgtccatcgc ggtgagtgcg caacaatatc atgtgcccgg ggcagagaca
    10141 actgtttcca gtttcgctca ggcccggctc cgcccgcgcc tcaaccggtc ccccaaaagg
    10201 actaggaaga agcacacggc ggcaaccagg atgatagtgg cgccggaagt caggttgtaa
    10261 gcataggaaa tccccagccc ggtcagcgtg aacaggcagt tgagaaccac cgagaagacc
    10321 atcatggatc gaagggagcc gcagtagcgc tcggcgatgt agggaggaat ggtcaagagg
    10381 gcaatcacca ggatcaaccc caccacccgg atgaccatca ccacggacag ggccatcatg
    10441 gccaggagca ggaagtacag ccctttgacg ggaaccccca ccgcaaggga gaattcttcg
    10501 tcgaaggaca atgccaggaa atcattgtag aaaaaagaga ccagcccgat gaccgcaagg
    10561 tccacggccc ccatcagcag caggtccgag gtcggcaccg tgaggatgct cccgaaaaga
    10621 tagctcatca ggtccacgtt atagcccgac gtcagatcga tgaggatgat tcccagggcc
    10681 attcccaccg cccacagaac cccgatgatg gtgtcggccc gctgcttgtt ccggatggtg
    10741 accaaggcca tgaggaagga aatcatcagg gaaaacccgg ccgctcccaa caggggggaa
    10801 aatccgaaaa aaaaggccat tcccacccct ccgtaggcgg catgggcaat ccctcccgag
    10861 atgaacacaa ttcggttgac gaccaccagg gttccgatca gtccgcacgc gatgccggca
    10921 agcagaccgg ccagaagtgc gttttgcatg aattcgaact ggagcacctc gatcatgtca
    10981 gtggtcctcg tgctttcgca ggacccggtg gggcagcccg tgggcgatca gctccaccgg
    11041 gcaatgatag gccttctcga tcatctccga ggtgatctcc gccgcatcgt ggtgatacag
    11101 ggtctggctc aaacaggcta ccgatttgat atagcttgat aaaacgctca agtcatggct
    11161 tacgaccacg acggtcatgg attcattgag ctccttgaga aagtcgtaga gttcggtctg
    11221 gaattccttg tccgtgctcg ccgtaggctc gtccatgaac aggatgtccg gctgagccgt
    11281 cagtgcccgg gcgatgtaca cccgctgccg ttgcccgccc gagagctttc caatgggcct
    11341 gtcccggtac tcccacatgc cgaccctttc cagggagctt tgggccacgg accgatccgc
    11401 cgtcgagtac ctgcgggaac ggcccggctg acccagccgc cccatgaggg tcacgtccaa
    11461 aaccgagatg ggaaagccct tgttgacgct tgtgtcctgg ggaacatagc cgatacggtc
    11521 ggcaatcttt cccggttcct tcccgtagat gcggatggtg ccccgcgacg gggtgaggat
    11581 tcccaggaga agcttgagaa acgtggtctt gccgctcccg ttgggaccca ggatcgccag
    11641 aaaatccccg tgctcgattc gcaggtttac accccgcagc acaaggtttc cgtcgtagga
    11701 aaaccacagg tcgtcgactt cgatggcgaa agcgcctgca atcattggtt cggtccttct
    11761 ctttggatcg aacccggccc ccggggcaac gggttcgagt gcccgaaaac ctccgaggag
    11821 gaattcgttc ctcaacggtt ttccaagcga agcccctccc acgaccgggg agaagccttc
    11881 ggaattccac ggcaatttcg ggaagccccg ggaccccgaa ttccagggtc gtgaaacccg
    11941 ccggttgctc cgttccgact caattcccga gagagggcat gttgcggagc acgaggttgg
    12001 tttcgggcag agatcgatcc gggaaggatt ctttttgcct gaagggacgg gggcctccca
    12061 cattccaaaa aaacccgcac cggaagaata gtttacaaac actcggaatg caactatctg
    12121 tatccatgga aacgctttgc ttggccggaa acggctgccg aaacttcccg gacggtgaaa
    12181 cgggtgggag gaaacaaacc cattgcccaa tgatttgaag aaaacctatc cccggattcg
    12241 ggaatccatg ctccgctggt tccgggacaa tcggcgggac ctgccgtggc gcaggtctta
    12301 cgatccctac cgggtttgga tctcggagat catgctgcaa caaacccggg ttcagaccgt
    12361 gctgccgtac tatgagcgat ggatggaacg cttcccccac gtacgttccg tggccctggc
    12421 acccgaagag gaccttctcc ggtgctggga gggcctcggg tactactctc gagcccgaaa
    12481 cgttcacagg acctcgaaga tcctggtgga ggcctacggc ggggaattcc cccgggacta
    12541 tgattccgtg cgacgtcttc cggggattgg agcctacacg gcaggggctc tcatgagcat
    12601 tgccttcaac gaagatttcc ccgtggtgga cggaaacgtg aaacgggtct tccaccgggt
    12661 tttcaacctt ggaaacggcc ggggagccca cgaatccgat cggacatact gggcccttgc
    12721 ggcggagctc cttccccggg ggcaggcgcg ggatttcaac caggcactca tggaactggg
    12781 agccctggtg tgccttccct ccaatccttc ctgtccgcgg tgtcccctcg aggggttctg
    12841 tgcctccctg ggggcgggcg cggtgcacga aaagcccccg aggacccgca agaagacctc
    12901 cgccatcgag gtttccgtgg gactgctgtt tcgtggggaa gaagtgttta tccagaaaag
    12961 gcctcccgag gggctcatgc ccaacctgtg ggagttcccc ggaggcaaga tccgggacga
    13021 tgagaccccc gaagaggccc tggtgcggga gtttcgggaa gaattgaatc tgggggtccg
    13081 ctgcttggac ccggtggccg tcattcggca tgcgtacacg tcctttcggg tgattctcca
    13141 tgcctatcgc tgcgcgcttc gggagggtgg gcaagagccg gtgctcaggg ccgccgtgga
    13201 agctcgctgg gtcgggcttc cccaattgga cgactacgcg tttcccgctg ccaatcgaaa
    13261 actgatcgaa atcctcgtgg gccgacgtca ccccgacggg cggtcccggg gaaaggaagc
    13321 ctgatatgtc gaatctttcg tcccttgcgg gtttcgtagt ggcggggatg ctgctttccc
    13381 tgcttttggg ctgtgcgggg cgagaccttc gacccgggca ggcggaaacg gccggaggac
    13441 ccatcaggcc gggggacatc gtcgaggctc gaagcggaat gcgcatttcc gcggaatccc
    13501 tttcggacag gctcgcccaa gtgcagatcg tctacggggg agagacccac accagcccgg
    13561 cggaccaccg ggtgcagctc gagatccttc gaagtctcca tgcaaagaat ccccggttgg
    13621 tcctggccat ggaaatgttt ccccgggagg tccagcccgt gttggatcgc ttttcccggg
    13681 gcctgatcgc cctcgaggat tttcccgggg aagtgggatg ggaaaccgtg tgggggtttc
    13741 cgtttcctct gtatcgagat ctcctggaat ttgcccagca ccatgggctc caagtcctcg
    13801 ggttgaatgc cccgcacgcc gtggtgcaaa aggtggcaag gtccggcctg gcgtctctct
    13861 cccccgagga acgagcccgc atcgcccggg aattccgccc ggaaaacccc cgacaccggg
    13921 aatacgtccg caaggaattc gatgatcacg tgaaggaaac catcaaggac ttccagacct
    13981 tctacgaggc tcagctcacg tgggaggaga ccatggcgga aaccctggcg gatcacctgc
    14041 gctcagcacc cccgggagaa caggtggtgg tgatcgtggg gcgaggacac cttcactaca
    14101 agttgggaat tccccgcctg gtggcggaac ggcttgccca ttcgttcaag acggtgattc
    14161 cccttcccgt ggaccatgcg gaaggttcgt cggaccggga gctgggggat tactactgga
    14221 ttaccgaaaa aacggagccc gctcatcggg gccggttggg gatcctgatt cgtcccgact
    14281 cgtccgggga aggagtcgag gtcgtctcgg tcatcgcgga aagcccggcg gcgaaggccg
    14341 gtttcaggaa gggagacatc atcgtcgggg tcaacgacgt cgtcgtcaag acgccccagg
    14401 acctccatcg agccatggct cataaaggaa agatccacgc cgtctcggtc aagagaaacg
    14461 gcggggagga aacgatggtg gtggatctgt ctccttgaac ctttcgggaa agcccggtca
    14521 caaggggagc cggtgcgatg cccggaggcc gatggcgtgc aaagatcgca aaagacccga
    14581 tccccggggt cccgggaacg ggaacgcagt agtacggatg gacggcgacg gcagagtgta
    14641 gtcaattcta ccaagggaac catgggaaca aaggagcggg atcatggcga cggtaatgcc
    14701 cgaaggggaa gcaatcagga aagccatcaa gtggatctcc gaggaactaa aggagcatcc
    14761 ggaaaagacg ccgacgcagt tggtagggga ggcgaccctg cggtttgacc tctcgcccaa
    14821 ggagggggac ttcctgctgg gcttttacag caaggcgtcg gagtagggaa tcgcggaaag
    14881 aagagcgggt cggtgcgttc gggcgaaggc cccagaggtc gaagcgcctg gaccgggccc
    14941 cggcgggaaa ccctggggcg gacgcccccg gggggctgca taccccccgg ggcgatccat
    15001 gattcttgac attccggaca agagatacta taggcaaatc ctgcaaccga gtggggatgt
    15061 agctcagctg ggagagcgca gccttcgcaa ggctgaggtc gtcggttcga tcccgatcat
    15121 ctccaccaat cgattcatta aaatgcgaaa tccaaccaac cgggaggcct gatccccaaa
    15181 ttcacgggga tcaggccttt acattggggt catggtggaa ccgggaagcc cgcaacctct
    15241 ccggagttcc ggtctcacag gcggccggtt cgagtctatt cggttgaaca gggaggcata
    15301 cggcattgca gggtccgaag atcacgcggc tgaacttccc gaacaccggc tgcccgtctt
    15361 cgcccctccc gctcacaagg gtccttctcc gccgtacccc ataaacccgc acgcacccag
    15421 aggcaaaaaa gcggagaacg gagccggcgg caaatgcgat accctgcccg aagtctggtt
    15481 ctccaacagg aatttacgag taaaagggtc aaaaccgtag acaaacgcgg tagtgtaaga
    15541 tcttttgtgt aaattgtaag cagtagaaaa aaaggcgctt ttccattaa
//
LOCUS       DAOOVC010000093        15564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2536_length_15564_cov_25.829260, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000093 DAOOVC010000000
VERSION     DAOOVC010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15564
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_15564_cov_25.829260"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLM79_11915"
     CDS             complement(<1..923)
                     /locus_tag="PLM79_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOV87061.1"
                     /translation="MRRMKRFWLPVVVLLLSAVFRPASPVPAASMDRLTVSGAFLNPQ
                     MKPVKEVEIQVLVNGRVVKSMGKDEEIASGSKGAFSAQFQLPPGTLPASKVEIKAQKP
                     SWEPLPPTVLKVVESGVDDQGNRLFETARNFTLARAVTPAFWIATLILLGVYVVISFE
                     WMHRTLAALLAAALILFVTYTLGSYDKSFYILSFEDAMGAIDLNVIFLLLGMMIFVGV
                     LKKTGMFQFLAYKSYAWARGNVFILSVVLQVLTAVISAFLDNVTTMLLVIPVTIEIAL
                     TLRVHPLTFLIPQVFASNVGGTATLIGDPPN"
     gene            complement(1092..1724)
                     /locus_tag="PLM79_11920"
     CDS             complement(1092..1724)
                     /locus_tag="PLM79_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOV87062.1"
                     /translation="MVRVLVADDHAVVRHGLKLILAEEFHLLHFGEARNSQEVLEQTS
                     GQEWDIVILDITMPGRSGLDVLKELKQLHPKLPVLVLSMHPEDQFAVRVLKAGAAGYM
                     TKENAPEELVKAIKKVLAGGKYVSAALAEKLAFQIEIDREKQPHESLSDREYQVMRMI
                     ASGKTVKEIADELALSIKTISTYRTRVLEKLNMKTNAELTHYAVKNCLVD"
     gene            complement(1741..3918)
                     /locus_tag="PLM79_11925"
     CDS             complement(1741..3918)
                     /locus_tag="PLM79_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV87063.1"
                     /translation="MQLFPFSSLRSRLILLILLAVLPSLGLTLYTGLEEHQIQMAQVQ
                     ENTLRLTRLAAGDLVQVIEGARQLLIGLAQLPEVGRGDSEVCGTLFSNLLRQYPYYLN
                     LGVIDSAGNLSCSALPFQGPVSMGSRVYVRGAINTASFTISPYQIEKITGEATINFVY
                     PVLGGRDGGQSAVFAALDLKWFKQIELEAQLPKGAELIVVDHNGTILARYPEPNQWVG
                     QFVPAELIVQAAISRHGEPSLVETRGVDGVRRLYGFSVVRGSPDSDIYVSIGITKEAA
                     FGSVNRMFVRNMVGVGLVGVLALAAAWLGGNAFILRRVSRLVNATQKLASGDLSVRIG
                     PPYEKKDELCVLGRSFDEMAESLEQRTSQLHQAEVRYRTLVEQIPMITYIAPLGRVGN
                     PLYISPQVKTILGFSREEWLTDSFLWLKQLHPEDRERVLRDISNSAVNHRGGGFCLEY
                     RIYSKEQRLLWVRDEAVVVQNEDGQPQFLQGILLDITGRKSSEEQLRRSHERLRDLAA
                     HIEAAREDERTRIAREIHDELGQSLTGLKMDVAWVEKRLSASRDSGATPLLLDKIQSI
                     KQIIAATIQCVRKITTELRPGILDDLGLLAAIEWQAMDFQNRMGISCEFLTLPESLEV
                     DGKRSSSVFRIFQEILTNIARHANATRVFVCLKEEAGSLVLEVKDNGRGITEREVRGG
                     KSFGILGMRERALLLGGEFDIRGAQGQGTTVRVRIPLEAPTDN"
     gene            complement(4122..4406)
                     /locus_tag="PLM79_11930"
     CDS             complement(4122..4406)
                     /locus_tag="PLM79_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87064.1"
                     /translation="MSTLHLLGQFVYGFLIFFVFSGFVFMWKSAQTGGVVSHRGIFQM
                     VFALGCIFAFGFTSMDKAHLLWAVPLGYLISLTPLGNYVGRMVSAYGARK"
     gene            complement(4624..5937)
                     /locus_tag="PLM79_11935"
     CDS             complement(4624..5937)
                     /locus_tag="PLM79_11935"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOV87065.1"
                     /translation="MDEGTLLWLITGLTAVFFVVGVPIFLCIGVWTTLASLAIGFTIS
                     NIGITSYQGISSYALLAMPLFILTGDLIGAGGIAVKLSALAAAILRPIRGGMALATIM
                     TCSTFAAISGSNSATVATIGRIMIPEMQKQGYNPAFAAANAASGGIVGILIPPSVLMV
                     IYGFMTNLSVIDLFTAGWGPGLCVSLGLAGAALFSARKNKWGEVGPFVFKDVFKAFWG
                     AKFGILAVFLILFLIYGGYSSPTESAGVVAFYCLLAGMFLTRELKLRDIPEVFLSSGR
                     VNGMLAPVIAISIVLQQVFNIIGLHEGVYDLIHTFKAPSLMLLAMMAAIVFFGCIMES
                     ISVCIILSPIFAPIAVSMGMNPYHFALTFIVGLSLGFVTPPFGLDLFVASGITGIPYG
                     KIIKWIPPFLVGICLAWLAVAFIPAISLTLFKIVGGGGAAASSGF"
     gene            complement(6015..6551)
                     /locus_tag="PLM79_11940"
     CDS             complement(6015..6551)
                     /locus_tag="PLM79_11940"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOV87066.1"
                     /translation="MAKPKWLTYLDKNVENVLIFPMYFFMMMIMAIDVIRRNVVHTQW
                     AWGNYVCISLFVWFSWLGCSYNIKQRAHLRLSSLRDMLPKGAQFGLLMLDYVLWIAFA
                     AIAGYFSWFQIVRLYNAGSLVYGAEWIPQWVVPLCIPVSFTVIVFRVLQCAWEDIQAM
                     IKKEPLQTQPRGGLETQV"
     gene            complement(6777..8144)
                     /gene="dctP"
                     /locus_tag="PLM79_11945"
     CDS             complement(6777..8144)
                     /gene="dctP"
                     /locus_tag="PLM79_11945"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOV87067.1"
                     /translation="MKKEEEKKKSSLTEKTVSRRDFTRIAATFGFTSTFLAYHGLAQA
                     GTPPTAMMLAQAAKETEQERAKVKAKVKLRYGTAGHDIESTWVAKVGTYDFVRDLEER
                     TDGAIQVQLMEANSICGEMTCAEKCIQGIIDLYLASTNNASTTCPYLNILDFGALWPS
                     RASLYSFAMDYRSEDLLREPMRRLYGIEMLFGDYGLRGFFMSKVKYGQGTPALDTLDK
                     LKATGAKIRTTGTLFGLKSMQLMGVNPVAISYEEVVDAVRQGAIDGAEAWEIPFNMIH
                     FTEFTGQYLYLKYCSGNWVTGMNVKSLDRIPADLRDKFMESCYLAQVATLGKEEASIY
                     VKAGSGELADPPPGSEHWKCNIRNIQWSKEEMDKLEKVISPKHNPQPWAEWREKLNKM
                     YGRGDVYEKLYEIAHEVPASAYAIDIIPKRWWKPNPPWWNKPDTAPWRRGSGYFAMNL
                     ERRKQ"
     gene            complement(8234..8878)
                     /locus_tag="PLM79_11950"
     CDS             complement(8234..8878)
                     /locus_tag="PLM79_11950"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOV87068.1"
                     /translation="MSILAEIQKAVFDGDESKTIDLVNKALAEGIAAQEIVDNGLVKG
                     LHDCGAGYEAGQKFIPEMLMAAEAMKSSMKILKPHLSAGSEGGNLGKVVLATVEGDVH
                     DIGLELVATMLGSAGFDVVFMGPDVPTEKIIATVKEEKPKLLGLSALLTNTMDVMPFI
                     LKKLEKEGLRNSLKVIIGGAPVSQDFADRIGAEGYAYDAAAAVPVSKKLVSQLA"
     gene            complement(8893..10044)
                     /locus_tag="PLM79_11955"
     CDS             complement(8893..10044)
                     /locus_tag="PLM79_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOV87069.1"
                     /translation="MSNYYMYDRCKEVTPMERMFACMNGEKTDRVPIHVYPRWAGLEY
                     IGVTFKEAWEDPDLYVKGQIMAQERFQYDCVIDYDMMGPVEEAIGATLKYPEDDVPQI
                     AEPFIKTKEDMKKAKWKLDPRKDGNMPKVLYAIRRLKEELIKRGKGKQQVPIMTWGSC
                     PSRTAACLTGFKEWFLIVARDPDWAVELMENALDGWLQMVIAAHEAGADCVWVNDPVS
                     SADCISREHYEKLTQPYERKFIEAIRRETGMKIIFHPCGNWHDRLDLIYQNQADGYFL
                     GPMNIDLCVEKAVETNQKGLSRVKFLNGNYGAATKTSTADRKPVSYATTLKFGTPDEL
                     LVECKTVIDTVEGADIRCMLGPNCWEPKNTPYVNRDATVFAARKFGAYK"
     gene            complement(10663..>11823)
                     /locus_tag="PLM79_11960"
     CDS             complement(10663..>11823)
                     /locus_tag="PLM79_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87070.1"
                     /translation="PTVYPTVYPTVYPTVYPTVYPTPGTVLKDRNWAITDIQAKLLCF
                     LIQEESRTTSRANLQKALDLSANTIKYALKALLSEGFIISTKRYAKGRFQGFSYTLND
                     SLCAEFLKKRGSHENQESTNPVYPTVYPTVYPTVYPTVYPTDPEPPQSVPDRLPDRLP
                     DRLPDLDPSSSSNIYIYKTTTTREDPSTRPSTRPSTRPSTRPTECEIEQVLNTVPELG
                     YWREKGLTARQVEEWMKISDTNLSDMIQSLCHCRYEMVDMKLEETKPVENVFNWFFRM
                     IERVGHYPAPKGYKSFLAKRIERERQLIQDREREIAEIEAIREKKRSQERELAFQRMM
                     NEPEGELFRACVDRLPPMAKQIDRNGRMFEEVMRSTFEEYLREKEQDHVITS"
     assembly_gap    11824..11923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12161..12979)
                     /locus_tag="PLM79_11965"
     CDS             complement(12161..12979)
                     /locus_tag="PLM79_11965"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOV87071.1"
                     /translation="MGGIVISVVNNKGGVGKTTATVNLAEALARRNQRVLVIDMDTQC
                     NATSLLLPTDVRIRNSLYELLDPEETEERNIEDFIHPTKLGNVFCIPNVSDTASLEPI
                     MIHRALTQKNPEVFFKLRRMLRQHIQENFDFAIIDNPPNMGTFVLCSLYASDFIVVPV
                     KAGSAFSVEGLIKAINLIDSVRDNGNKDLRFLRLLINKADRRTFISRANIEQIRQSFS
                     EQRVFNTIVPVNTAFERAESQGSTIFQYDQTSSGARSYRELARELLAIFDKNGD"
     gene            13425..14027
                     /locus_tag="PLM79_11970"
     CDS             13425..14027
                     /locus_tag="PLM79_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOV87072.1"
                     /translation="MKNRNHPPKGSRIAVDPIRRLSDIENIKKMLQGRKSRDLLLFTI
                     GVNSGLRSGDILAIKLNQVRHLKPGDRLVIKERKTGKQNILMVNKAIYKAIRDYVKDV
                     QSTSLEEEFLFKSRKGKNQPIKVESLNRMIKEWCRSINLDGNFGAHTLRKTFGYIQRK
                     YYGVGVEVLAKRFNHSNPSTTMKYLGISDQEVDAILMHDI"
BASE COUNT         3439 a         4189 c         4310 g         3526 t
ORIGIN      
        1 atgttggggg gatcgccgat gagggttgcg gtgccgccca cgttggacgc aaagacctgg
       61 gggatgagga aagtcagggg gtggaccctc agggtgaggg cgatctcgat ggttaccggg
      121 atcaccagga gcatggtggt gacgttgtcc aggaaggccg aaatcaccgc ggtcaagacc
      181 tggagaacca cggacaggat aaacacgttc ccccgggccc aggcatagga cttgtacgcc
      241 aggaattgga acatgcccgt cttcttcaac actcccacga agatcatcat ccccaggagc
      301 aggaagatca cgttgaggtc gatggccccc atggcgtcct cgaaggagag gatgtaaaag
      361 gacttgtcgt agcttcccag ggtgtaggtg acaaacagga tgagggccgc ggccaggagg
      421 gccgccaggg tccggtgcat ccactcgaag ctgatgacga cgtacacccc caacagaatg
      481 agggtggcga tccagaaagc aggggttacc gctcgggcca gggtgaagtt gcgggccgtt
      541 tcaaaaagcc ggtttccctg atcgtccacc cccgactcca caactttgag aaccgtgggc
      601 ggaaggggct cccaggaagg cttctgcgcc ttgatctcca ccttggaggc aggaagagtc
      661 cccggcggca actgaaactg cgccgagaac gcccccttgc tgcccgaggc gatctcttcg
      721 tccttgccca tggacttcac cacccggccg tttaccaaga cctggatttc cacctccttc
      781 accggcttca tctgcgggtt cagaaaagcc ccggagacgg tgagccgatc catggaagcc
      841 gccggcaccg gagaggcggg ccggaagacg gcggatagga gtaacacaac aacaggcagc
      901 caaaatcgtt tcattcgacg catagaacca atcctctcgc gtagttgtga atcacatctt
      961 ttcatgacgt ataagcgagg tttccgggca aggtggaagg tagcatcgag gaagggaaag
     1021 tctgtcggct tgttcctgat atttctgtag gacttcacgg tttgcgtttg tcggcctttg
     1081 tcctacagga atcagtccac aagacaattc ttgaccgcat agtgggtcag ttccgcattg
     1141 gtcttcatgt tcagcttctc caagaccctt gttcgatacg tgctgatcgt tttgatgctc
     1201 agggcgagct cgtcggcaat ttccttcacg gtttttcccg aggcaatcat gcgcatcacc
     1261 tggtattccc gatccgagag gctctcgtgg ggctgttttt cacgatcgat ctcgatctgg
     1321 aaggccagtt tctccgccag ggcggcactc acgtactttc ctccggccag cactttcttg
     1381 atggctttga ccagttcctc gggggcgttt tccttggtca tgtaaccggc agctcccgcc
     1441 ttgaggacac gcaccgcaaa ctgatcttcc gggtgcatgc tcagaaccag aacgggaagc
     1501 ttcggatgca gttgcttcaa ttctttgagc acatccagcc cgcttcttcc gggcatcgta
     1561 atatccagga tcacgatgtc ccattcctgt ccggaggtct gctccaacac ttcctggctg
     1621 ttgcgcgctt ccccgaagtg aagcaggtga aattcttccg ccaggatgag cttcagtccg
     1681 tgacggacca ccgcgtggtc gtctgccacc agaactctta ccatggtccc ccccgatccc
     1741 ctagttgtcc gttggtgcct cgagcggaat gcgaaccctg accgtcgttc cttgtccctg
     1801 ggccccccgg atgtcgaatt cccctcccag caaaagggcc ctttcccgca tccccagaat
     1861 gccgaaggac ttgcctcctc ggacctctct ttcggtgatc ccgcgcccat tgtccttcac
     1921 ctcgaggacc aggcttcccg cttcctcctt caggcatacg aaaaccctgg ttgcattggc
     1981 atgtcgggcg atattggtca ggatctcctg gaagattcgg aaaacgctgg aagaccgctt
     2041 cccatccacc tccaggcttt ccggaagggt caggaattca cacgaaatcc ccatcctgtt
     2101 ctggaagtcc atggcctgcc attcgatggc cgccagaagc cccaagtcgt ccagaattcc
     2161 cgggcgcaac tccgtggtga tcttgcggac acattgaatg gtggcggcaa tgatctgttt
     2221 gatcgactgg atcttatcca gcaacaacgg cgtagctccg gaatccctcg atgcggagag
     2281 tcttttctca acccaggcca cgtccatctt gagtcccgtg agagattgcc ccagctcatc
     2341 atggatctcc cgggcaattc gagttctctc atcttcccgg gccgcctcga tgtgcgcagc
     2401 aaggtcccgc agccgctcat gggagcgtct caattgttcc tcgctggatt ttcgtccggt
     2461 aatgtccagc aggatgccct ggagaaactg cggctgccca tcctcgtttt gaaccaccac
     2521 ggcttcgtcc cggacccaca gcaggcgctg ctccttcgag tagatccgat attccaggca
     2581 gaagcctccc ccccggtggt tcacggcgct gttggagatg tcccggagca cccgttctcg
     2641 gtcctcgggg tgaagctgct tgagccacag gaaggaatcg gtaagccatt cttcccggga
     2701 aaacccgaga atcgtcttga cttgagggct gatgtagagg ggattgccca cccttcccag
     2761 gggggcgata taggtaatca tcgggatctg ctcgaccagg gtccgatacc ggacttccgc
     2821 ctggtggagc tgggaagtcc tctgctccag ggattccgcc atttcatcaa aggaacgtcc
     2881 caggacgcaa agttcgtcct ttttttcgta cggcggaccg attcggacgc tcaggtcccc
     2941 ggaagccagt ttttgggtgg cattcaccag gcgggaaacc ctccgaagaa tgaaagcgtt
     3001 tccacccaac catgcggcgg caagggccaa aacacccacc agccccacac cgaccatgtt
     3061 cctgacaaac atgcggttga ccgagccaaa ggccgcctcc ttggtgatgc cgatgctcac
     3121 atagatatcc gaatccgggg accctcgaac cacggaaaat ccataaagtc ggcgaacgcc
     3181 gtccacccct cgcgtctcca ccagggaggg ttccccatgg cgggaaatcg ccgcctgcac
     3241 gatgagctcc gccggaacaa attggccgac ccactggttg ggttccgggt accgggccag
     3301 gatggtcccg ttgtgatcga caacgatcaa ctccgcccct ttgggcaact gcgcctcgag
     3361 ttcaatttgt ttgaaccact tcaaatccag ggccgcaaaa acggcgctct gacccccatc
     3421 ccgacccccc aaaaccgggt acacgaaatt gatggtcgcc tccccggtga ttttctcgat
     3481 ttgataaggg ctgatggtaa agctcgcggt gttgatggcg cctcgaacat acacccggct
     3541 tcccatcgat acgggtcctt gaaaaggcag cgcactgcag gaaaggtttc ctgcggaatc
     3601 gatgactccc aagttcaggt agtagggata ctgcctcaga aggttcgaga acagggtgcc
     3661 gcacacctcc gaatctccac ggccgacctc cggaagctgg gcaaggccga tgaggagctg
     3721 tcgcgcccct tcaatcacct gcaccaggtc cccggcggca agccttgtga ggcgaagggt
     3781 gttctcctgc acctgggcca tttgaatctg atgttcttca agtcccgtgt acagcgtcaa
     3841 gcctaaggag ggaagcaccg ccaggaggat caacaggatc agccggctgc gaaggcttga
     3901 aaagggaaaa agctgcatgg gttcctcgat cacccccgga ggcgcatcgg ttggacggat
     3961 cgttgttcag gcggctgaaa tgctgcttca gcattgtgaa aatggcatgg aaaacctcaa
     4021 aagaccatcg ggaacagtct gattttcggg taggatttca aggatgaggg ttgtcggtcc
     4081 ttctcctaca aacgccggaa ggacccgggc gatgcagacc tttattttcg ggccccgtag
     4141 gcagaaacca tgcggcccac ataattcccc aggggagtca acgagatcag gtatcccagg
     4201 ggaaccgccc agagaagatg cgccttgtcc atggaggtga acccaaacgc aaagatgcat
     4261 cccagggcaa aaaccatctg gaaaatcccc cggtgactga cgaccccccc ggtttgtgcc
     4321 gacttccaca tgaagacaaa ccccgaaaac acgaagaaga tcagaaaccc gtagacgaat
     4381 tgtcccagga gatggagagt gctcatggtc cggtttccct cgatatcatg cccttgcatg
     4441 tccgtacatt gcgtgggtcg ctcttccgcc acgactccgt caaagaagaa aagccctgcc
     4501 ccggaaaggt cccgggaggc agggctttct attgtatccc gtcttgtcct tgggaaaaca
     4561 ggagaaagcg ggggaaattc ttaccttccg tgggaggtat tccccttggg taaggggaaa
     4621 tcctcagaac cccgagcttg ccgctgcacc tccgcctccc acgatcttga agagggtaag
     4681 ggagatggcc gggatgaagg caacggcgag ccatgccaga caaatcccga ccaggaaagg
     4741 gggaatccac ttgatgatct tgccgtacgg aattcccgtg atcccggagg ccacaaacag
     4801 gtccagccca aagggaggcg tgacgaaccc cagggacaat cccacgatga acgtcagggc
     4861 gaaatggtag gggttcatcc ccatggaaac ggcgatgggg gcaaagatcg gcgataagat
     4921 gatgcacacc gagatggatt ccatgatgca tccgaagaaa acaatggccg ccatcatcgc
     4981 caggagcatg aggctcggcg ccttgaacgt atggatgagg tcataaactc cctcgtggag
     5041 cccgatgatg ttgaagacct gctggaggac gatggaaatg gcgatgaccg gcgccagcat
     5101 gccgttgacc cggccgctcg aaagaaacac ctccggaatg tccctcagct tgagctccct
     5161 ggtcaggaac atgcccgcca gaagacagta gaaggccacc acccccgcag actcggtggg
     5221 cgagctgtag cccccgtaga tgagaaacag gatcaggaaa accgcaagaa tgccgaactt
     5281 ggcgccccag aaggccttga acacatcctt gaacacaaag gggcccactt ccccccattt
     5341 gttcttccgg gccgaaaaca aggcggcacc ggcaagcccc aggctcacgc aaagccccgg
     5401 tccccagcct gccgtgaaca gatcgatgac gctgaggttg gtcatgaacc cgtagatgac
     5461 catgaggacg ctgggaggaa tcaaaatccc cacgatcccg cccgaggcgg cgttggctgc
     5521 cgcaaatgcg ggattgtaac cctgcttttg catctccggg atcatgatgc ggccgatggt
     5581 ggccaccgtg gccgagttgg agccggaaat ggcggcaaac gtgctgcacg tcatgatggt
     5641 ggccagggcc atcccccctc gaatgggacg aaggatggcg gccgccaggg ccgagagctt
     5701 gacggcgatc cctcccgccc cgatgaggtc ccccgtcagg atgaagaggg gcatggccag
     5761 gagggcgtag ctgctgatgc cctggtagct ggtgatcccg atgttgctga tggtaaaccc
     5821 gatggccagg gaggccaggg tggtccatac gccgatgcag agaaaaatcg gcactcccac
     5881 cacgaaaaac acggccgtaa ggccggtaat aagccagagc aaagttcctt catccatgga
     5941 tgccttcctt tctcgcccag agagatcggc actccccccc gatccctcaa tccgtcctaa
     6001 tcgcccggtc cctgctacac ctgggtctca agccctcccc tgggttgggt ctggagcggc
     6061 tccttcttga tcatggcctg gatgtcttcc caggcgcatt gaaggacccg aaagacaatg
     6121 acggtaaagg agaccgggat gcagaggggg acaacccatt gcgggatcca ctcggccccg
     6181 tacaccaggc tcccggcgtt atagagccgt acgatctgga accaggaaaa gtagccggca
     6241 atggcggcaa aggcaatcca gaggacatag tccaacatca agagtccgaa ctgggcccct
     6301 ttcgggagca tatcccgaag actgctcagc cgaaggtggg ctcgctgctt gatgttgtag
     6361 gaacaaccca gccaggagaa ccacacgaac aaactgatgc atacgtagtt tccccaggcc
     6421 cattgggtgt gcaccacgtt tcgacggatg acgtcgatgg ccatgatcat catcatgaag
     6481 aaatacatgg gaaatatgag aacgttctcg acatttttgt cgagataggt aagccatttt
     6541 ggttttgcca tagcgttccc caccttgccc cttcaagcgc aggagcatgc ttgcaggttt
     6601 ttcgataatt ggatgacggg ccgcctccat ccgatcgcga acagacagga tgaaacagac
     6661 cggtgagtcc ggagaaaatg tccgatgagg aagcgaaggt tccacccaac ttccggaaac
     6721 cacaaaacat ccgtcttcct cccaagacgg atgttttgtg gacatcaaga cccctcttat
     6781 tgctttcttc tctccaagtt catggcgaaa taaccgcttc ccctcctcca gggggccgtg
     6841 tccggcttgt tccaccaggg cggattcggc ttccaccatc tcttgggaat gatgtcgatg
     6901 gcgtaggccg aggccggaac ctcgtgggcg atttcataga gtttctcgta gacgtctcct
     6961 cgaccgtaca tcttgttgag tttctctctc cattcggccc agggttgggg gttgtgcttg
     7021 ggagagatca ccttctcgag tttgtccatt tcttccttgg accactggat gtttcggatg
     7081 ttgcatttcc agtgctcgct gcccggaggg ggatcggcga gctcgccgga gccggccttg
     7141 acgtaaatgg aggcctcttc ttttcccaag gtcgccactt gcgccaggta gcaggactcc
     7201 atgaacttgt cccgaagatc ggcggggatc ctgtccaggc tcttgacgtt catcccggtg
     7261 acccagtttc cggagcagta cttgagatac aggtattgac ccgtgaattc cgtaaaatga
     7321 atcatgttga aggggatttc ccaggcttcg gcgccgtcga tggctccctg ccgcacggcg
     7381 tccaccactt cctcgtagga gatggccacg gggttcaccc ccatgagctg catgctcttc
     7441 aggccgaaga gggtacccgt ggtgcggatc ttggctcccg tggctttgag cttgtccagg
     7501 gtatcgagag ccggtgttcc ttggccgtat ttgaccttgc tcatgaagaa accgcgaagg
     7561 ccgtagtcgc cgaaaagcat ctcgatgcca tacaaccttc tcatgggttc ccggagcaga
     7621 tcttcgcttc ggtagtccat ggcgaaagaa tagagagaag cccggctggg ccagagggcc
     7681 ccgaagtcca gaatgttcag gtaaggacag gtggtggatg cattgttcgt ggaagccaga
     7741 tataaatcga tgatgccctg gatgcacttt tccgcgcagg tcatttcacc gcagatggag
     7801 ttggcttcca tcagttgaac ctggatggcc ccgtcggtcc tttcctcgag gtccctgaca
     7861 aaatcatacg tgccgacctt ggcgacccag gtggattcaa tatcatggcc tgccgttccg
     7921 taacggagct ttaccttggc tttgactttg gctcgttcct gctcggtctc cttggccgct
     7981 tgggcgagca tcatggccgt gggaggcgtg cctgcctgag ccagaccatg ataggcaagg
     8041 aacgtcgagg taaagccgaa cgtggctgca atcctggtaa aatctcttcg ggatactgtc
     8101 ttttcggtaa gagaactctt cttcttttct tcttccttct tcatcaggtc cccctttcga
     8161 ttcctaatgt tgatgcctgt acccggttcc tcctgatgga agcaagtata ccaggtacag
     8221 gcaattcaag gaactatgcg agctgactca ccagcttctt ggaaacaggg accgctgccg
     8281 ccgcgtcgta ggcgtaacct tccgccccga tccggtcggc aaaatcctgg gacaccgggg
     8341 ctccgccgat gatcaccttg agggagtttc tcaatccttc tttctcgagc ttcttgagaa
     8401 tgaagggcat cacgtccatg gtattggtca gaagggccga gagtcccagc aacttgggct
     8461 tttcttcctt gaccgtggcg atgatcttct ccgtgggaac atccggtccc atgaagacca
     8521 cgtcgaagcc ggcgctcccg agcatggtgg caaccagttc caggccgatg tcgtgtacgt
     8581 cgccttccac ggtggcgagt actaccttgc cgagattgcc tccttccgac ccggcggaca
     8641 ggtgaggctt caagatcttc atggacgact tcatggcttc cgcggccatg agcatctccg
     8701 gaataaactt ctgcccggct tcatatcccg cgccgcagtc atggagtcct ttcacaagcc
     8761 cgttgtccac gatctcctga gccgcaattc cttcggcaag ggccttattc acgagatcta
     8821 tggtcttgga ctcgtctcca tcgaaaactg ccttttgaat ttccgctaat atgctcacct
     8881 tgttcctcct ggctatttgt atgcgccaaa cttccgtgcc gcgaagaccg tcgcatcccg
     8941 gttgacgtag ggggtgtttt tgggttccca gcagttggga ccgagcatac atcgaatgtc
     9001 cgcaccttcg acggtgtcga tgacggtctt gcactcaacc agtaactcgt ccggggtccc
     9061 gaacttcagg gtggtcgcgt aggaaaccgg cttccgatct gcggtggagg ttttggtggc
     9121 cgcaccgtag ttgccgttga ggaatttcac tcgagacaaa cctttctggt tcgtctccac
     9181 cgctttttcc acgcaaaggt cgatgttcat ggggccgaga aagtacccgt ccgcctggtt
     9241 ctggtaaatg agatccaggc gatcgtgcca gttgccgcag gggtgaaaaa tgatcttcat
     9301 gccggtttcg cggcggatgg cttcgatgaa ttttcgctcg tacggttggg tcagtttctc
     9361 ataatgttcc cgggagatgc agtcggcgga ggagacggga tcgttgaccc acacgcagtc
     9421 ggcaccggct tcatgggctg cgatgaccat ctggagccat ccgtccagag cattttccat
     9481 aagttctaca gcccaatccg ggtccctggc aacaatgagg aaccattcct tgaagccggt
     9541 caaacaggcg gccgttcggg acgggcagct tccccacgtc atgatgggca cttgctgctt
     9601 tcctttcccc cgtttgatca gttcttcctt gagacggcga atggcgtaaa ggaccttggg
     9661 catgtttccg tctttgcggg ggtccaactt ccatttggcc ttcttcatgt cttccttggt
     9721 tttgatgaag ggctccgcga tctgaggaac atcgtcttca ggatacttca gggtggctcc
     9781 aatggcttct tccacgggtc ccatcatgtc gtagtcgatg acgcaatcgt attggaaccg
     9841 ctcctgagcc atgatctgcc cctttacgta cagatccgga tcctcccagg cttccttgaa
     9901 agtgaccccg atgtattcca ggcccgccca ccgtggatat acatgaatgg gaactcggtc
     9961 cgtcttctcc ccgttcatgc atgcaaacat tctctccatg ggagttactt ctttacagcg
    10021 atcatacatg taataattgg acatttgatt cttcctccta agagtttgtg ctatgaaatg
    10081 ttggtgggtt acgagggtgg atcgttcatt ctgccttgat ggctccatta ccaagatcgc
    10141 taagctcatc gaatgaccag acaacaaatt ccccttcgca agtccctcaa gcgaaggcaa
    10201 cgttccttca acaagcctcc tttcttcgca aaaaaaacgg gacgatgtgt tgcttccaaa
    10261 ccccacaaat ttcctaggtc tttatcaaat ttagtgccaa attttagtcc cccctcaaat
    10321 cccacctttc ccatttccgc actccatcct cccgggaaaa accccttgcc cctacctcag
    10381 tgcacgacaa ttttgccggc gtcccctcaa gcacccacct cctcccgact ctccatacgc
    10441 tcccgaatgc cgttttctct tttgtagcat tgtacaaaag atgtcatgtg acatgtatgt
    10501 taggttacgg tgtcaacaac cagcacacct tcggtcggag accttgaccg tactcgcata
    10561 agaagagatc cttcgtcccc caactctctc cccatcttca tggagccagt tgatcgactt
    10621 tctgaggccg gtctgaaatc ttcgtccgtc tcaatcgaga tatcatgatg ttattacatg
    10681 gtcttgttct ttctcccgga ggtactcttc gaacgtactt ctcatcactt cctcgaacat
    10741 gcggccattt cggtcaattt gcttggccat gggcggaagt cgatccacgc aggcccggaa
    10801 aagctctccc tccggttcgt tcatcatcct ctgaaaggcc agttcccttt cctgactccg
    10861 cttcttttcc cgaatcgcct cgatctccgc aatctctctt tcccgatcct ggatcaattg
    10921 gcgctctcgt tctatccgct ttgccaggaa agatttgtac cccttgggag ccgggtaatg
    10981 ccccaccctc tcaatcatcc tgaaaaacca attgaacacg ttctccaccg gcttggtttc
    11041 ttccagcttc atatccacca tttcataccg acaatgacac agcgactgaa tcatatcgga
    11101 gagattcgtg tccgatattt tcatccattc ttcgacctgt cgtgcggtca gtcccttttc
    11161 tctccagtac cctaattccg ggacggtgtt caggacttgt tcgatttcac actcggtcgg
    11221 tcgggtagac ggtcgggtag acggtcgggt agacggtcgg gtagacgggt cctccctagt
    11281 agtagtagtt ttatatatat atatattact actactacta gggtcgaggt cgggtagacg
    11341 gtcgggtaga cggtcgggta gacggtcggg tacagattgg gggggctcag ggtcggtcgg
    11401 gtagacggtc gggtagacgg tcgggtagac ggtcgggtag acggtcgggt agacgggatt
    11461 tgtggattcc tggttttcgt gactgcctct ttttttcaga aactcggcgc aaagagaatc
    11521 attgagagtg taggagaacc cctgaaaacg accctttgcg tagcgcttcg tgctgatgat
    11581 gaacccttcg gataggagcg cttttagcgc gtattttata gtgttggcgc tgaggtccaa
    11641 tgctttttgg aggttggcgc gactggtggt tcgcgattct tcttgaatga gaaaacaaag
    11701 aagttttgct tgaatgtccg taatggccca attgcgatct ttgagcactg ttcccggggt
    11761 cgggtagacg gtcgggtaga cggtcgggta gacggtcggg tagacggtcg ggtagacggt
    11821 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtagacg gtcgggtaga
    11941 cggtcgggta gacggtcggg tagacggtcg ggtagacgag gaggcggatg gatcgttgga
    12001 agggggggga tcgtcagggg gagtggccgg ttcctgggat gaaattttct cgattttctt
    12061 atttgcctcc tcgaggtctt cttttcgtac gagttgatat ccctcgagga gagacagacg
    12121 gcgccgggtg gagtttgcca tggtgcgctc cccttttcgg ttaatcgcca ttcttgtcga
    12181 agatggctag aagctctcgg gccagttcgc ggtaggatcg tgcaccggac gatgtctgat
    12241 catactgaaa aatggtggat ccttgggatt cggcacgttc gaaagcggta ttgaccggca
    12301 caatggtgtt gaagacccgt tgttcggaaa acgattggcg aatctgttca atgtttgccc
    12361 gactgataaa ggtgcgccga tccgctttgt tgatgagaag gcgcagaaaa cggaggtctt
    12421 tgtttccgtt gtcccggacg ctgtcgataa ggttgatggc cttgatgagt ccctccacgc
    12481 tgaaagcgct tccggccttg acggggacca cgataaaatc ggaggcgtaa agggaacaca
    12541 agacaaaggt tcccatgttg ggcgggttgt cgatgatggc gaagtcaaag ttctcttgga
    12601 tgtgttgccg aagcattctc cgcagtttga agaagacctc cgggtttttc tgagtcaggg
    12661 cccgatgaat catgatgggc tccaaggatg ccgtatccga aacgttgggg atacaaaaaa
    12721 cgtttcccag cttcgttgga tggataaagt cttcgatgtt gcgttcctcg gtttcttcgg
    12781 ggtcgagcag ttcgtatagg ctgtttcgaa tccgcacatc ggtgggaaga agaagggagg
    12841 ttgcgttgca ctgggtgtcc atgtcgatca caaggacccg ttgatttcgc ctggcgaggg
    12901 cttccgccag gttcaccgta gcggtggtct tgcccactcc tcccttgttg ttgacaacgg
    12961 agattacgat gccgcccatg gggacctccg tgatccgatg tggaaagtat gagaatggtg
    13021 ttaaatttcg ccaataaact tagattgtaa gtatcgagga tgtcaatcga aagttatgta
    13081 tgatctgaga aaaatcgatg aaggaatgaa aaacttccgg ttgaaggcag gataaaggaa
    13141 gcccctcgat cccccgcccc ttcctcaccg gatggttttc cttgtcgccc cgcatccttc
    13201 gatttggata ccgcacaaga ttgatcgagt tcgaaagaac tgtcgatacg aggaacgcca
    13261 accttttgta aaaagttaag attgggttgg agaagattta tcccgtaaat agaatcggtt
    13321 ggtcatgaaa tcgagaggaa ttccggaatt cgtccggttg gaatgtaatc tattgaagcc
    13381 tttcacatct atacttaaca ttcatcctca ggaaaggcgt attgatgaaa aataggaatc
    13441 atccacccaa aggcagccga attgccgtag acccaataag aagactctcg gatatagaaa
    13501 atatcaaaaa aatgttacag ggaaggaaat cgagggatct tttactgttc acgataggtg
    13561 taaacagcgg gttgagatca ggagacatcc ttgctattaa gttaaaccag gtgagacatt
    13621 tgaagccggg tgaccggttg gtgatcaaag aacgaaagac tggaaaacaa aacatattga
    13681 tggttaacaa ggcaatttat aaagcgatac gagactatgt gaaggacgtt caatcgactt
    13741 cattggaaga ggaatttctt tttaaatcca ggaaaggcaa aaaccaaccc ataaaggtgg
    13801 agagcttgaa tcgtatgatt aaggaatggt gccgatccat caacctggac ggaaactttg
    13861 gggcccacac gctccggaaa acgttcgggt acatccagag gaagtactac ggagtcggcg
    13921 tcgaggtcct tgccaaaagg ttcaatcatt ccaatccgtc caccacaatg aagtacctgg
    13981 gaatcagcga tcaggaggtc gacgcgatcc tgatgcatga catatgatgg ggcacccgac
    14041 agcgacatcc gagacggtcc gaaaacatct ttgatgatcc cgcaaaaagt cgttctttcc
    14101 aaaacggcac ctcgtgaatt cataccgggt tgcgcccagg ggcgatctga catgctgagt
    14161 gaagattttg agcaaacggt tgaatcctgc gggatggcag tttcggcgtg aatgcaactt
    14221 cgtatcaatc ggttaggatc gaagaacgca cttttcgggc ctttttagga gaccatcatc
    14281 ttttttgttg cgatgcaaag aatgggaagg ttcatggacc cccgaaagtc gtcggagccc
    14341 catcagggac gactgcaccg ggatagccgg ccgagacgtt gttgtacgga ttggcccagt
    14401 tgggtgcaaa gggattcacc cgataagtgc tctgggcgaa cgggttcacg gtggtaagcg
    14461 gattgggcgt aaaggggttg atcgtcgtcg gggggacaaa tgaaacactg ctttggccca
    14521 tagggctcga ggcggtgctc ggcatgacga aaaatctttt tgaccgaata gaagaagacc
    14581 cttgaggatt cgacggggtt gtggggttga tgcctgcatt cgtggaagaa tactcggcaa
    14641 aaccggcacc tccttcggga acggtcccgg cagtggcgga gggaggacca aaagcggagg
    14701 gggaacttac gggattgatt cccgatgggc ttgtggaatt tggaatagag gcgctgccgg
    14761 tggtggtgcc ttgaccaaag aggttgcccg tagagctgaa aatggaggcc ataggattgg
    14821 aggacatggt ggttgccact gaggaagttg aggaggtgga tgtaggcgag gtggctggat
    14881 tgaggaggct tccatatcga ccataatggc tggaaatgtt gtccgggcgg taaaggcttc
    14941 caacaccata aagggatggg gaagaagtac ccgtggagga ggacgcgtca ggagtgggcg
    15001 ctttgtagat acggggagcc cgagcttttc cacccccgcc cccggcgttt cggacctggc
    15061 ctgcaaaagc aacgcttgca tgaagaaacg acacggacaa tataaaaaca aataattttc
    15121 tcacgagcac cctctccttt ccaggacctt cgagggcatt gtatagccct gcatcggaag
    15181 atatcaaccg ctaaatcccg caaaccccgc cccatcctcc tggaaattcg gagggagaaa
    15241 ggatcttccg ggcccgcaaa ccatcgctgg aggcatggaa tggtctctca ttttgcagga
    15301 tcgttcatgc tcgcggggag attccgaagg ccgtttgttt agttcgttca gggtcatagt
    15361 tctcatcgag ggctggaaaa cgagggcctt tccagctttt agcccggagc agagccgcat
    15421 tgggcgtggc cttgatcaaa aagatatggt atgtagctga atgtttacgg gggaggagat
    15481 gatcgatctc aaatgtggtc gggatgcttc ggagttagga attcgaaccc atgaaggatg
    15541 gaacatcacc gctttcttgg acca
//
LOCUS       DAOOVC010000094        15279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2598_length_15279_cov_6.926038, whole genome shotgun sequence.
ACCESSION   DAOOVC010000094 DAOOVC010000000
VERSION     DAOOVC010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15279
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2598_length_15279_cov_6.926038"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="PLM79_11975"
     CDS             <1..1525
                     /locus_tag="PLM79_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HOV87073.1"
                     /translation="SAPPGKGEEFGNPHSRNNTADHSKFKELQQEFKSGPEVTRACLT
                     CHTEAARQIQKTIHWTWIVPDAKPGVKMGKGGNVVNNFCVSLYSNEPRCTSCHAGYGW
                     KDKDFDFASQDKVDCLVCHEQSGTYRKFPAGAGHPADEPKKMGDQTFNPPEWSKVAQS
                     VALPTRKNCGTCHFFGGGGEGVKHGDLDAGLMRPEKALDVHMDADGLNFTCTRCHTTS
                     KHDIAGRPYKMSEITDRRSLLNDDQMDRITCYSCHSETPHKEGSKANDHTNRVACQTC
                     HIPSFARKNSTKMFWDWSQAGLRDDTGKPVVRMKDGRAVYDGMKGSFVWERNVVPEYF
                     WFNGKLQYTLLPDKIDPSGTVSINRVTGSPDDVNSRIYPFKVHRGRQPYDVRNNTLVA
                     PHLFGKDDAAYWKTYDWSKAIRAGMDYAGIPYSGEYGFVDTSYLYQTTHMVAPKENAL
                     SCNECHVRGGRLEKIRGVYIPGRDSATGMNLLGWGGVFAASLGVIGHGTLRFVRRKK"
     gene            1553..2182
                     /locus_tag="PLM79_11980"
     CDS             1553..2182
                     /locus_tag="PLM79_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOV87074.1"
                     /translation="MERLYLYTRFERFWHWAQAALILSLVVTGLEVHGTYRLLGFQRA
                     VEIHNGAGIAWFILYVFIPFWQATTGEWKHYIPTTKKLMAVVFYYGYGIFRGDAHPVP
                     KSERNKHNPLQRLTYLAIAVVLIPLQVFTGLLYYSYNSWPQLGLSIHLRTLATIHTAG
                     AFGLLIFLIVHIYMTTTGHTLTCHLKAMCTGWEEVPKQLPVEGTLDKAA"
     gene            2338..2541
                     /locus_tag="PLM79_11985"
     CDS             2338..2541
                     /locus_tag="PLM79_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87075.1"
                     /translation="MALDEPRDTDEVKTINEFTWVMEKSLQQQTGLVTVDFVNYGMGS
                     SFLVTSEVPVNKSKAGCGTCCSH"
     gene            complement(2703..5111)
                     /locus_tag="PLM79_11990"
     CDS             complement(2703..5111)
                     /locus_tag="PLM79_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOV87076.1"
                     /translation="MGRGHTKWFLLGVLGVLCLALFRTYSFGEMKSVGAVGEFKSTDT
                     LEDLKAKIRDNGYGFTVGRNWVFDMTPEQKERFFSRRPPKVPRAGPEKGMGPLAKHLG
                     KTALPSSFDWRSYNGRSYIGPVRNQGNCGSCYSFAAAAAAEGTYNVAVGNYDGDAEDF
                     SEAFIAWCLGKYGPYSEHFGGCDGADYDYYELEALTQEGIIRESDFPYTVSDPGSCKH
                     WSDPTLVFQNWYRIPCGDIDAMKTALMTYGPLDAAVYVGSAFEAYESGIYEDTRTSCG
                     ANPCYYTATNHAVSLVGWDDNGGDGYWILRNSWGTSWGEQGYMRIKYTSAVVSCEAAY
                     LVFNCNDTKLVVQGLAPSANFSVPPDSPVLVKAQVSNECGGVIAGAQVTASFSNGDAG
                     IPLFDDGNHGDANANDGIYANNWTPVTVQGSTVITLNASKNGYTAASTTISGAVGQTW
                     TEGFEGSLFPPTDWTLGQTNPRQTWNSSPDNPHGGAHCAAVYYDEELEAQDELLMTPT
                     LTFTNTGTLSFWSSGSIHWGVEPFDNYDVEVWLIFDDWDGGSGDDVLLGKADDSWAGD
                     WAYARSTFDLRPHLQSKPARIGFRYVGTDGAAVYLDDVRVSTGSGQGTGSLRVTLTPD
                     AAVTAGARWKVDDGSWKQNGDTVAGLAAGTHNVTFRKIAGWMGPETQTVTVKAGETGD
                     LSAAYRKKPTVKVTASDHQASEAGRNKGTFTFTRTGHKSEALTVSYSVKGTAVSGKDY
                     SALSGKVVIPRGKASAKVVVSPVNDKRYEGRETVIVTVKSDSSYLVGSPSRAKVFIYD
                     DDGN"
     gene            complement(5519..5800)
                     /locus_tag="PLM79_11995"
     CDS             complement(5519..5800)
                     /locus_tag="PLM79_11995"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV87077.1"
                     /translation="MSVKVEDKLFLNRYRVDEESHLHIADPRVCTVQCSDQPCLYLCP
                     ANVYRLEKDRISVAYEGCLECGSCRIGCPHFNIEWRFPQGGFGIRHKFG"
     gene            complement(5797..7101)
                     /locus_tag="PLM79_12000"
     CDS             complement(5797..7101)
                     /locus_tag="PLM79_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87078.1"
                     /translation="MDEQFDVAVVGAGPAGISAACILAEKGVRTVVLERGEYPGAKNL
                     FGGVLYGHDLAQIVPDYRDRNCPIERNIVESRIWYLSRNGGYSLSYRDRVFELQGKFN
                     AFTTGRARFDRWFAEQAKDRGALILCSTVVTDLLRDSNRRVIGVRTDRPDGDLRARVV
                     LLADGINSPLAKKTGFRPEPKPAEVALSVKEVIGLPEEVIEERFGVESGSGVTVEILG
                     DVTWGMNGVAFLYTNKRSLSVGIGANLLHFSQNRVRPYEVLEAFKQHPMVAPFIKGGK
                     PQEYTAHWLAEGGFDTIPQLCGDGFLITGDSAMLFNALHREGTNLAMASGRMAAETVL
                     EAMDRGDFTRRGLEGYGRRLQESYVLRDMKKYRRFPHLLQEHKEIFTTLPELSSLAAR
                     EMLTVNGVPKKEKQRAIWNAVRRKMSPFRLLGLLWDAWRSVR"
     gene            complement(7216..8469)
                     /locus_tag="PLM79_12005"
     CDS             complement(7216..8469)
                     /locus_tag="PLM79_12005"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOV87079.1"
                     /translation="MAKNTKGIEVAEILPDRCIACRICIGECPTAAIELTPDGVAHID
                     PELCIGCGKCHESCPVDAVKFERKMKRRLAREERGALPEPVGGYDGVAVFIEVAGGAG
                     VQVSWELIGKARELARALNSRVLGFVLGSGVDAVAREAVEYGCDDVYVLDDPRLLQYL
                     PAVYGRALADLCEKVRPEILLMGATHLGRDLAAVVATLLKTGLTADCTGLSIEEETRL
                     LLMTRPTFGGNIMATIFCEHRRPQMSTVRPMVMRPPEKDPRRQGTIHHPPWEPPPMDL
                     PVLVDFIQGVSPGSGIDITRSPALVVAGRGACDPSSFHLIEELAQAVGGTVACSRPVV
                     ESGFMPYERQVGQTGRTVAPKLYIGIGVSGAIQHLVAIQGAEKILAVNSDPKAPIFKV
                     ADVGIVGNYLEVVPELIAQLKKRTG"
     gene            complement(8476..9312)
                     /locus_tag="PLM79_12010"
     CDS             complement(8476..9312)
                     /locus_tag="PLM79_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV87080.1"
                     /translation="MHIVCCIKQVPDTAEVKIDPDTNTLVRTGVESISNPYDIVAVEA
                     ALRIRERYGGRVTALSMGPPQAESSLREALAMGVDDGILLSDRAFAGSDTLATSYTLG
                     CAVRRLSLDDPVTLVLCGKQAIDGDTAQTGPGIATRLGFTQFTYVTEVDWVDLENGRI
                     QVKRKLEDGLEVIRGPLPALLTVELELAKPRRASLPALVRSLRAPVANWDAKAISASP
                     KELGLKGSPTWVRRISSPPAKTGGPLFYAGEDLRESVAQTLDILFSDKTFAAKFMNRW
                     EA"
     gene            complement(9567..9815)
                     /locus_tag="PLM79_12015"
     CDS             complement(9567..9815)
                     /locus_tag="PLM79_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOV87081.1"
                     /translation="MASRVREIDLTKIHPNLRFLCDNEAIDELCLSIKCHGQEEPIHI
                     CFMGSEFRIVHGEKRWRACKKLGMTRIKAIIVEEEEVD"
     gene            10225..12714
                     /gene="leuS"
                     /locus_tag="PLM79_12020"
     CDS             10225..12714
                     /gene="leuS"
                     /locus_tag="PLM79_12020"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOV87082.1"
                     /translation="MNSRYVPGKIEKKWQDIWAQEKLFQVKEDESREKYYLLEMFPYP
                     SGRIHMGHVRNYSIGDVVARFLTMNGCNVLHPMGWDAFGMPAENAAIKAKIHPARWTY
                     ENIDYMRSQLKKLGFSYDWTREFATCDPSYYRWEQVFFLKMLERGLAYRRSSYVNWCD
                     TCRTVLANEQVEGGACWRCDQPVVQKEMEQWFFKITEYVEELLDYTYKLPGWPERVLT
                     MQQNWIGKSHGSKLLFPLAAHEGEITVFTTRADTLFGATFMSLAPEHPLVETLCAGKP
                     QEKEVLAFVERTKRAKRSDRVSELLEKEGVFTGSYCINPATRENIPIYVANFVVMEYG
                     TGAVMAVPAHDQRDFEFARKYGLPIRVVIKPAEAGDVPSPESLEAAFEDDGILVNSGK
                     FSGLASAAARDAVTAWLAEEKLGDFSVQYRLRDWGISRQRYWGAPIPVVHCPRCGIVP
                     VPEEDLPVVLPTNIELLPNGASPLPTCESFVKTPCPRCKGPARRETDTMDTFVESSWY
                     FARFACADYDRGPLDRQRVKYWLPVNQYIGGIEHAVLHLLYSRFFTKVLRDMGYVDID
                     EPFLNLLTQGMVIKDGAKMSKSKGNVVDPDEMIEAYGADTVRLFCLFASPPERDLEWS
                     DQGVDGASRFLSRVWRMVYDNLASLKGAGRLGSEEKLSGGLEDLRRKTHQTIKKVTED
                     IRDRFHFNTAIAAVMELVNLLYQVLDNKEEKRAYWSVVREAADSVILMLSPFVPHIAE
                     ELWQALGNSGSILHAPWPRWNEKALASAEVLIVIQINGKLRSRITVPADFSEEAIKQA
                     ALADPKTLEYLGSKTPKKVVLVPKKLVNIVV"
     gene            12880..13422
                     /gene="lptE"
                     /locus_tag="PLM79_12025"
     CDS             12880..13422
                     /gene="lptE"
                     /locus_tag="PLM79_12025"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOV87083.1"
                     /translation="MKRLCPKLFRLCCLVAVVLFNGCGYHFTGEGPGPRPGLTKIAVP
                     LFENKTSEPELESLFATALRKEFLQKGSMQVVPVDQAEAIFRGTVKSISTSAVSHLEV
                     EKTIESRLYVTLDIRCEDARDGTILWQDRNFTYYKVFLQYLSPKNPNPIIGYESRRAA
                     LEFLAGEMSVRIHDRFLNSF"
     gene            13425..14438
                     /gene="holA"
                     /locus_tag="PLM79_12030"
     CDS             13425..14438
                     /gene="holA"
                     /locus_tag="PLM79_12030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOV87084.1"
                     /translation="MLPGELHQRIERGDIAPVYLFLGEAAFLMEEAWEHLVRTCFSKT
                     ARRFKGERFLAKECPASDVIEKLSTVPMFGSRRLILVSGVEAWNKDQRNALTSYLKAP
                     SPHGCLVLRAAQSKGIESLESCVASVGAVVHFKTPAEKDAPRWLRERAQYHGKQLSPD
                     GAMFLVEQVGVDLFRLERELEKLAAYVGSRSRIELRDVQEVVGSQRFFTVFEMLRFLG
                     RRKAHKALVSLRSLILLGEAPLAILGMLARQVRILWQIKEGIEEGLSTADLAKKLRLP
                     PYVAKNLAGEASLFSHGNLMEIHQALREADLALKSTGTAPEKTLEGLVLKFCGKKPSG
                     FSP"
     gene            complement(14484..14753)
                     /gene="rpsT"
                     /locus_tag="PLM79_12035"
     CDS             complement(14484..14753)
                     /gene="rpsT"
                     /locus_tag="PLM79_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOV87085.1"
                     /translation="MANHKSAIKRAKQSEARRQRNRARKTRMKNVVKSLETAIQSKAV
                     DAAVEGLKEAISIIDKTAAHGVIHKNKASRKISQLTRKVNSLQAV"
     gene            complement(14943..>15279)
                     /locus_tag="PLM79_12040"
     CDS             complement(14943..>15279)
                     /locus_tag="PLM79_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87086.1"
                     /translation="VWNVYAMGSGEWGPWWQACKMVVGRDGKIKDGECTDSMGDKSPI
                     VGGAVNLSRNCLLSGEFKFKDVEEGIVFTATINHGTLEHAKQMAAGVGKNDNAQIFNF
                     TAIRQPKNK"
BASE COUNT         3025 a         4526 c         4612 g         3116 t
ORIGIN      
        1 atcggcgccg ccggggaagg gggaggagtt cggaaatccc cattccagga acaacacggc
       61 ggaccactcg aagttcaagg aactgcagca ggaattcaaa tccggtcccg aggtgacccg
      121 ggcgtgtctt acctgccaca ccgaggctgc ccggcagatt cagaagacca tccactggac
      181 ctggatcgtt cccgacgcca aacccggggt caagatgggc aagggcggaa acgtggtgaa
      241 caatttctgc gtcagtctgt attccaacga accccgttgc acctcgtgtc atgccggtta
      301 tggatggaaa gacaaagatt ttgattttgc gtcccaagac aaggtggatt gtcttgtgtg
      361 ccacgagcag tcggggacgt accggaagtt tcccgccggt gccggtcatc ctgccgatga
      421 acccaagaaa atgggagacc aaacgttcaa tccgcccgaa tggagcaagg tggcccagtc
      481 ggtcgctctg cccacgcgca aaaactgcgg cacctgccac ttcttcggtg ggggcggcga
      541 aggggtcaag cacggcgacc tggatgccgg cctcatgaga ccggagaagg ccctggatgt
      601 ccacatggat gccgatgggc tcaatttcac ctgcactcgc tgtcacacga cctcgaaaca
      661 cgacatcgcc gggcgcccct ataaaatgtc ggaaattacc gaccggcgaa gccttctcaa
      721 cgatgatcag atggaccgga ttacctgtta ttcctgccac tcggagactc ctcacaagga
      781 aggctccaag gcaaacgatc acaccaatcg ggtggcctgc cagacctgcc atattccctc
      841 ctttgcccga aaaaactcca cgaagatgtt ttgggactgg tctcaggcgg gcttgcggga
      901 cgacacggga aagccggtgg tgcggatgaa ggacggaagg gcggtctacg acgggatgaa
      961 agggagcttc gtgtgggaaa ggaacgtggt ccccgaatat ttttggttta acgggaaact
     1021 ccagtacacc ctgttgccgg acaagatcga cccgtccggg accgtgtcca tcaaccgggt
     1081 aacgggttcc cccgatgatg tcaattcccg gatctatccc ttcaaggtcc atcgggggcg
     1141 tcagccctac gacgtccgga acaacaccct ggttgcccct caccttttcg gcaaggacga
     1201 cgccgcctat tggaagacct atgactggag caaggcgatc cgggcgggga tggactatgc
     1261 cggaattccg tacagcgggg aatacggctt cgtggacacc tcgtatctct accagaccac
     1321 ccacatggtg gcccccaagg aaaatgcctt gtcctgcaac gaatgccacg tccggggcgg
     1381 aaggcttgaa aagatccggg gggtctacat cccgggacgg gacagcgcga cgggcatgaa
     1441 cctgctggga tggggagggg tctttgccgc atccctgggc gttatcggtc acgggaccct
     1501 gcgattcgta cgccgcaaga agtagaaagg agaacccatc atgcaatccg ccatggaacg
     1561 actttatttg tatactcgtt tcgaaaggtt ctggcattgg gcgcaggcag cacttatctt
     1621 gagtctcgtt gtgaccggat tggaagtgca cggcacctac cggctgctgg ggtttcagcg
     1681 ggcggtggaa atccataacg gcgccgggat cgcctggttc attctctacg tgttcattcc
     1741 tttctggcag gcgaccacgg gggagtggaa acattacatc cccaccacca agaaattgat
     1801 ggccgtggtc ttttattacg gttacgggat cttccggggg gacgcccatc cggtgcccaa
     1861 gagcgagcgc aacaagcaca accctttgca gcggctgacc tacctggcca tcgccgtggt
     1921 tctgatcccg ttgcaggtgt ttacaggtct gctgtattac tcgtataact cgtggccgca
     1981 actgggtttg agcatccacc taagaacttt ggcgacaatt cacacggcgg gagcatttgg
     2041 attgttgatt tttttgattg ttcatattta tatgaccacg acaggtcata cactcacctg
     2101 tcatctgaag gccatgtgca ccggatggga agaagttccc aagcagcttc cggtggaagg
     2161 aactctggac aaagcagcct gaaggcaagc tccttcagca gaaaggaagc ggaaaagcga
     2221 atggttacgg tatcggagaa tgcagaaagc gccttgaggg attacttcaa agggaagaac
     2281 gccagtccca tccggatttt tcttcagtcc gggggctgag gaggaccttc gttatccttg
     2341 gctctggacg agccgagaga cacggacgag gtaaagacca tcaacgagtt cacctgggtg
     2401 atggagaaat ccttgcagca gcagacaggc ttggtgacgg tggactttgt caactatgga
     2461 atggggtctt ccttcctcgt cacctcggaa gttcccgtga acaaatccaa agccggttgc
     2521 ggcacctgct gcagccactg aagcgcccgt cttttgccca ccaaagaaaa gaggggaggc
     2581 ccggtcgctc tcccctcttt tctttcctga cgatcttcct gggttcgttg tcggctttct
     2641 gcacagtccc tctttttacc ggagctcctg acacatcccg aacttcttga ccccggcgaa
     2701 gactaatttc cgtcgtcgtc atagatgaaa accttggccc gggaggggga acccacgagg
     2761 taggatgagt cggacttcac ggtgacgatc acggtctccc ggccctcgta tctcttgtcg
     2821 ttgacgggag acaccaccac tttggcggaa gcctttccgc ggggaatcac caccttgccc
     2881 gaaagcgccg agtagtcctt gccggacacc gcggtgccct tcaccgagta ggataccgtc
     2941 agggcttccg acttatggcc cgtgcgggtg aaggtgaagg tccctttgtt ccgaccggcc
     3001 tccgaggcct gatgatccga ggcggtgact ttcaccgtcg gtttcttccg gtatgcggcg
     3061 gaaaggtccc cggtttctcc agccttgacg gtgacggtct gggtctcggg acccatccaa
     3121 cccgcgattt tgcgaaaggt cacgttgtgg gtgcccgcgg cgagacccgc aaccgtgtct
     3181 ccattctgct tccaggaccc gtcgtccacc ttccagcggg cccccgccgt gacggccgcg
     3241 tccggggtga gggttactcg gagcgatccg gtgccctggc cggaaccggt tgagactctg
     3301 acgtcatcca ggtagaccgc cgctccgtcg gtacccacgt accggaatcc tatgcgcgcg
     3361 ggttttgact gaagatgagg cctgaggtcg aaggtcgacc gggcgtaggc ccagtctccc
     3421 gcccagctgt cgtcggcttt tcccagcaag acgtcgtccc cggaacctcc gtcccaatcg
     3481 tcgaagatca gccacacttc cacgtcgtag ttgtcaaaag gctcgacccc ccaatgaatg
     3541 ctgcccgaag accaaaaact gagggtaccc gtgttggtga aagtgagggt cggggtcatg
     3601 aggagctcgt cctgggcctc cagctcctcg tcgtaataga cggcggcgca gtgggccccc
     3661 ccatgggggt tgtccgggga agagttccat gtctgacgcg ggttggtctg cccgagggtc
     3721 cagtccgtgg gaggaaacag gctcccttcg aacccttcgg tccaggtttg ccccaccgcc
     3781 ccggaaatgg tggtgcttgc cgcggtgtag ccgttcttgc tcgcgttcaa ggtgatgacc
     3841 gtcgagcctt ggacggtgac gggggtccag ttgttggcgt aaatgccgtc gttggcattc
     3901 gcgtcgccgt ggtttccgtc gtcgaagagg gggatgccag catcgccgtt gctgaagctt
     3961 gccgtcacct gggccccggc gatcactcct ccgcactcgt tgctcacctg tgccttgacc
     4021 aaaaccgggg aatcgggcgg aaccgagaag ttcgccgaag gggcaagtcc ttggaccacc
     4081 agcttggtgt cgttgcagtt gaaaaccagg taggccgcct cacaactgac tacggcggag
     4141 gtgtacttga tgcgcatata gccctgctcg ccccagctcg ttccccaact gttgcgaaga
     4201 atccagtacc cgtccccgcc gttgtcgtcc catcccacca ggctgacggc gtggttggtg
     4261 gccgtatagt aacagggatt ggcgccgcag gaggtcctgg tgtcctcgta gattccactt
     4321 tcataggctt cgaaggcgct gcccacgtag acggcggcat ccaaggggcc gtaggtcatg
     4381 agggcggttt tcatggcatc gatgtcccca cagggaattc gataccagtt ctgaaagacc
     4441 agggtcgggt ccgaccaatg cttgcaggaa cccggatcgc tgacggtgta agggaagtcg
     4501 ctctcccgga tgatgccttc ctgggtcagg gcttcgagtt cataataatc gtagtcggca
     4561 ccgtcgcatc cgccgaagtg ttccgagtag ggtccgtatt ttcccaggca ccatgcgatg
     4621 aaggcttcgg aaaagtcctc ggcatcgccg tcgtagttgc cgacggcgac gttgtaggtg
     4681 ccctccgccg ccgcggcggc ggcgaaggaa taacaggagc cgcaatttcc ctggttgcga
     4741 accgggccga tgtaggatcg gccgttgtag cttcgccagt caaaagagga cggaagcgcc
     4801 gtcttgccca gatgtttggc aagaggtccc atgccctttt ccggtcccgc ccggggaacc
     4861 ttgggcggac gacgcgagaa aaagcgttcc ttctgctcgg gggtcatgtc gaaaacccag
     4921 ttgcggccca ccgtaaagcc gtacccgttg tcccggatct tggccttcag gtcctcgagg
     4981 gtgtcggtgg atttgaattc cccgacggcc ccgacactct tcatctctcc aaaagaatag
     5041 gtccggaaca acgcaagaca gagaacaccg agaactccca gcagaaacca ctttgtatgc
     5101 ccgcgtccca taagatttcc tcgcgaaatg tcgtctcagg gtccgtccga atggaaacgg
     5161 gtcccccctt tttcaggcgg atcacccttc ccggggctga ggggaccttg cctcttgcac
     5221 aaacggcttg agcccgaaga tggatgaaaa cgctctctct tcatgggttc ttctctgcca
     5281 accgaaagat gcttacggca ggctcgcccg gatccttgag cccaaagcca gggccgggat
     5341 ctttccgggc ggacggtttc ccggattccg gggacgggcc tgaacggagc cgccctgggg
     5401 aaagcgaggc cggtcccgac attacgcaga atcccggggg cgaggtccga cggaggcggc
     5461 ctgcaccgga gaagcggctc tgccgccggg atgacgcggg ggtcgttcgc cccgactctc
     5521 agccgaactt gtgccggatg ccgaaccctc cttgagggaa ccgccattcg atgttgaaat
     5581 gggggcatcc gatccgacaa gagccgcatt ccaggcatcc ctcataggcc acgctgatgc
     5641 ggtccttttc caaccggtag acgttggcgg gacataaata gagacagggc tggtccgaac
     5701 actggacggt gcagacccgg gggtccgcga tgtgcaggtg cgattcctcg tccacccggt
     5761 agcgattcaa aaacagcttg tcttcgacct tgacgctcat cgcacgcttc tccatgcatc
     5821 ccacaagagg cccaggagcc ggaacgggga catcttccgt cgtacggcgt tccaaatagc
     5881 cctctgcttt tctttcttgg ggacgccgtt caccgtgagc atttcccttg cggccaggga
     5941 gctcaattcc ggcaaggtgg tgaaaatctc cttgtgttcc tggagcaggt gaggaaatcg
     6001 ccgatatttc ttcatatccc ggaggacata ggattcttgg agacgcctcc cgtatccctc
     6061 cagtccgcgc cgggtgaagt ccccccggtc catggcttcc aacaccgttt cggcagccat
     6121 ccttcccgag gccatggcca ggttggttcc ttcccggtgc agggcgttga agagcatggc
     6181 ggaatctccc gtgatgagga acccatcccc gcatagttgg ggaatcgtgt cgaagcctcc
     6241 ttcggcgagc cagtgggccg tgtactcctg gggctttccg cccttgatga agggagccac
     6301 catgggatgc tgtttgaagg cttcgagcac ttcgtaaggc cggacccggt tttgtgagaa
     6361 atgaagaagg ttggcgccaa tgccgacgga gagggatctc ttgttggtat agaggaaggc
     6421 gaccccgttc atcccccacg tgacgtctcc caggatctcg acggtaaccc cggacccgga
     6481 ttcgaccccg aaacgctcct cgatgacttc ttccgggagg ccgatcacct ccttgacgga
     6541 cagggcgacc tcggcgggtt tgggctcggg ccgaaacccc gtctttttgg ccagggggga
     6601 gttgattcca tcggccagga ggactacccg ggctcgaagg tccccgtcgg ggcgatccgt
     6661 cctcaccccg atcacccgac gattcgagtc ccgcaggagg tccgtcacca ccgtgctgca
     6721 caggatgaga gccccccggt cctttgcctg ttcggcaaac caccggtcga agcgggcccg
     6781 gcccgtggtg aacgcattga attttccctg gagttcgaag acacgatccc gataggacag
     6841 gctgtatccc ccgtttcgag aaaggtacca gatgcgggat tcgacgatgt tgcgttcgat
     6901 gggacaattg cggtctcgat aatcgggcac gatttgggcg agatcgtgcc catagaggac
     6961 tcctccgaaa aggttcttgg ctccggggta ctcgcctcgc tcgagtacga ccgttctcac
     7021 tcccttttcg gccagaatgc aggcagcgga gatgcccgcc ggtccagcgc ccacaacggc
     7081 tacgtcaaat tgctcgtcca tggattcgtt cctgctacgg ttccctctgt ctcgcggagg
     7141 cccctcgtgg aaaattcccg ggacaacttc cgccctgccg acaagcccgg aaatggaagc
     7201 cggcatgccg ggcgatcagc ccgtccgttt cttcaactgg gcaatgagct ccgggaccac
     7261 ctccaggtag ttgcccacga ttcccacatc ggccaccttg aatatgggag ctttggggtc
     7321 cgaattgacg gcgaggatct tctcggcgcc ctggatggct accaggtgct ggatggcccc
     7381 cgaaaccccg atcccgatat agagtttggg ggccacggtc cggccggtct gacctacttg
     7441 ccgctcgtag ggcatgaagc ccgattccac cacgggccgg gaacaggcca ccgttcctcc
     7501 caccgcctgg gccagttcct cgatgaggtg aaaggaggag gggtcgcaag cccctcttcc
     7561 cgcgaccacc agggccgggg agcgcgtaat gtcgatcccg ctccccgggg agactccctg
     7621 aatgaagtcc acgagaaccg ggagatccat ggggggaggc tcccacggtg gatggtgaat
     7681 ggtcccttgg cgccggggat ctttctcggg cggccgcatg accatgggcc gaacggtgct
     7741 catctggggc cgcctgtgtt cgcagaaaat ggtggccatg atgttgcccc cgaaagtggg
     7801 gcgcgtcatg agcaggagac gggtttcctc ctcgatggaa agccccgtgc agtctgcggt
     7861 gagccccgtt ttgagaaggg tcgccacgac cgccgccaaa tctcggccca ggtgggttgc
     7921 gcccataagc aggatttccg gtcgcacttt ctcacaaagg tcggcaaggg cccttccgta
     7981 cacggcgggc aagtattgga gcaggcgcgg atcatccagc acgtacacgt cgtcgcaccc
     8041 gtattcgacg gcttcccggg ctacggcatc cacgccgctt cccagcacga acccgaggac
     8101 tcgggagttt agtgcccggg caagctcccg ggccttgccg atgagctccc aggaaacctg
     8161 aactccggcg cctccggcca cctcgatgaa gaccgccact ccgtcgtatc cgcccaccgg
     8221 ctccgggagg gcccccctct cctcccgggc aagccgccgt ttcatctttc gctcaaactt
     8281 caccgcgtcc accgggcagc tctcgtgaca cttgccgcat ccgatgcaca gttccgggtc
     8341 gatgtgggcg accccgtccg gagtgagttc gatggctgcg gtcggacact ccccgatgca
     8401 aatccggcag gcaatacagc gatcggggag gatctccgcg acttcgattc ctttggtatt
     8461 cttggccatg ccggatcagg cctcccatcg gttcatgaat ttggctgcaa aggtcttgtc
     8521 ggaaaagagg atgtccaggg tctgggcgac ggattcccgg aggtcctcgc ctgcgtagaa
     8581 cagggggcct ccggtcttgg cggggggaga ggaaatccgg cgaacccagg ttggggagcc
     8641 cttgaggccc agttccttcg gggaggcgga aatggctttg gcgtcccagt tcgcaacggg
     8701 ggcgcgcaag ctgcggacca gggccggaag ggaggctcgt cggggttttg cgagctcgag
     8761 ctcgaccgtg agcagggccg gaagggggcc gcgaatgact tccaatccgt cctccaattt
     8821 ccgcttcacc tggatgcgcc cgttttccag gtccacccaa tccacctccg tgacataggt
     8881 aaactgggta aagccgaggc gggttgcgat tcccggtccc gtttgggcgg tatccccgtc
     8941 gatggcctgt tttccgcaca agaccagggt tacgggatcg tccagggaga gtctccggac
     9001 ggcacagccc aaggtgtagc tggtggccag ggtgtccgac ccggcgaacg cccggtccga
     9061 gagaagaatc ccgtcatcga cccccatggc cagggcttcc ctcaacgagc tttccgcctg
     9121 gggcggaccc atggaaaggg ccgtgacccg gcctccgtac cgctcccgaa tgcgtagggc
     9181 cgcttccact gcgacgatgt cgtacgggtt ggaaatggac tccacgccgg tgcgcaccag
     9241 cgtattggtg tcggggtcga tcttgacctc ggcggtatcg gggacctgtt tgatgcagca
     9301 aacaatatgc atggatcatc ctcggcgaag gggtcttttc tcaggggcga agggaaggag
     9361 caccgatggc ccttcctgcg gcgaaccccg gacagggtcg ccgagtgccg ccggggaaat
     9421 caccgacttt gtggaaaggc gggcccgagc ctcctcttct gtcaaggggt cggaaggtga
     9481 gcatctcaag gatcggaatc cgcggggaaa gggaaaaagg ttcgaggaaa gaatgaacga
     9541 aagggagagc tcgagacggg aaaatctcaa tccacttctt cctcctccac aatgatggcc
     9601 ttgatccggg tcatccccag cttcttgcag gctcgccatc gcttttcccc gtgtacgatg
     9661 cgaaactcgc tgcccataaa acagatgtga atgggttctt cctgcccgtg acacttgatg
     9721 ctcaaacaga gctcgtcaat ggcctcgttg tcgcagagga agcggaggtt gggatggatt
     9781 ttggtgaggt cgatctctcg caccctggat gccatgggcc cctcctgttc gagcacgttc
     9841 ccgttgaaat tcgcacgggt cctttccaaa aaccttacgg gagaagtgtt tcgaaggcaa
     9901 gaatgaattg ccggatcccg ttccccccat gcccgcccga tagccggatg cactctgcgc
     9961 cctcccggtt ttcggccggg accccggttg tcgaggacgg tgtccgttgg aagcggccaa
    10021 ggtttcgcgg tcggggaagg gccggtatgc ccttctgcga tcccggggcg gaaggagagc
    10081 aggcacccgg gtcaaggttg ccaaacgggt ccgcctgtgc tagaaagtct ccacttttcc
    10141 agggtttgac acacgacagg gtggaagttc tcgacagacg gtggtttcac ccgcactcca
    10201 tagacgaacg ggaacaggtg atccatgaat tcaaggtatg tacccgggaa gatcgaaaag
    10261 aaatggcagg atatctgggc ccaagagaag cttttccaag tgaaggagga tgagtccagg
    10321 gagaagtact acctcctgga aatgtttccc tatccttcgg gtcgtattca tatggggcac
    10381 gtccggaact attccatcgg ggacgtggtg gctcggttcc tgacgatgaa cggctgcaac
    10441 gttcttcatc ccatggggtg ggatgccttc ggcatgcccg cggaaaacgc cgccatcaag
    10501 gccaagattc acccggcccg gtggacctac gagaacatcg actacatgag gtcccagctg
    10561 aaaaagttgg ggttttccta tgattggacc cgggaatttg ccacctgtga cccgtcctac
    10621 taccgatggg agcaggtctt cttcctgaaa atgctggaac gcggcctggc ctaccgtcgc
    10681 agttcctatg tgaactggtg cgacacctgc cggacggtgc tcgccaacga gcaggtggaa
    10741 ggaggggcct gctggcgatg cgatcagccc gtggtgcaaa aggaaatgga gcagtggttc
    10801 ttcaagatca ccgaatacgt ggaagagctg ctggactaca cctataagct gccggggtgg
    10861 cccgagcgcg tcctgaccat gcaacagaac tggatcggca aaagccacgg gagcaagctc
    10921 ctcttccccc tcgccgccca tgaaggcgag atcacggtct tcaccacccg ggccgacacc
    10981 ctctttggag ccaccttcat gagccttgcc ccggagcacc ccctggtgga aaccctctgt
    11041 gcgggaaagc cccaggagaa ggaagtcctg gcctttgtgg agagaaccaa acgggccaag
    11101 cgcagcgacc gggtctcgga actgctcgaa aaggaagggg tgttcaccgg cagctattgc
    11161 atcaacccgg caacccggga aaacatcccc atctatgtgg ccaatttcgt ggtgatggag
    11221 tatggaaccg gcgccgtcat ggctgtgccc gcccatgatc agagggattt cgagttcgcc
    11281 cgcaagtacg ggcttcccat ccgggtggtg atcaaaccgg cggaggccgg ggacgttcct
    11341 tctccggaaa gcctcgaggc ggccttcgaa gacgacggaa tcctggtgaa ttccgggaaa
    11401 ttcagcgggt tggcgtcggc agcggcaagg gatgccgtca cggcatggtt ggcggaggag
    11461 aaactgggag acttctcggt ccagtaccga ctgcgcgact ggggaatctc ccggcaacgg
    11521 tactgggggg ctcccattcc cgtcgtccac tgtccccggt gcggcattgt tcccgtccct
    11581 gaagaggacc ttccggtagt cttgcccacg aacatcgagc ttctccccaa cggagcttct
    11641 ccgctcccca cgtgcgaatc ctttgtgaag accccctgtc cccggtgcaa agggccggcc
    11701 cggagagaaa ccgataccat ggataccttt gtggaatcct cctggtactt tgcccgcttc
    11761 gcctgcgccg attacgaccg gggtcccctg gaccgccaaa gagtcaagta ttggctcccg
    11821 gtgaatcaat acatcggagg gatcgagcat gcggtcctgc acctcctcta ttcccgcttc
    11881 ttcaccaagg tccttcggga catgggctac gtggacattg acgagccgtt tctcaacctt
    11941 ctcacccagg gcatggtcat caaggacggc gccaagatga gcaagtccaa agggaacgtg
    12001 gtggatcccg atgaaatgat cgaggcctac ggtgccgata cggttcggct cttctgtctc
    12061 ttcgcttccc cccccgaaag ggacctggaa tggagcgatc aaggggtgga cggggcatcc
    12121 cggttcctgt cccgggtgtg gagaatggtc tatgacaacc tggcctccct aaagggggcg
    12181 gggcgtttgg gaagcgaaga gaagctttcg ggaggcctcg aggatctgcg tcgcaagacc
    12241 caccagacca tcaagaaggt caccgaggac attcgggacc ggtttcattt caatacggcc
    12301 atcgcagccg tcatggaact ggtcaacctc ctgtaccagg tcctggacaa caaggaggag
    12361 aagagggctt actggtccgt cgtgcgggag gccgcggatt ccgtcatcct catgctttct
    12421 cccttcgttc cccatatcgc cgaggagctc tggcaagcct tggggaactc cgggagtatt
    12481 ctccatgcgc cctggccccg gtggaacgaa aaggccctgg cctccgccga agtgctcatc
    12541 gtgatccaga tcaacgggaa actgagaagc cgcatcaccg ttcccgcgga tttctcggaa
    12601 gaagcaatca agcaagccgc tctggcggac ccgaagaccc tcgagtacct ggggagcaag
    12661 acgcccaaga aggtggtcct ggttcccaag aagctggtga atattgtcgt ctgagagaag
    12721 gcgatccgcc aatagtcccc gggttttgtc cagatcaatc cccaccgggc ctgcctggac
    12781 ggaggcaggg caggagcccc ggcgtaagcc gttgaatcca accggttccc caaacgcgga
    12841 cgcgccccgg cccaatgcct ccgcgcagga ggagaaatca tgaaacgcct atgtccgaag
    12901 ctcttccgcc tgtgttgcct ggttgcggtc gtccttttca acgggtgcgg ctaccatttt
    12961 acgggggaag gaccggggcc ccgcccgggc ctgacgaaga ttgccgtccc cctgttcgag
    13021 aacaagacgt cggagccgga attggagagt ctctttgcaa cggccctccg gaaagaattt
    13081 ctacagaagg gatccatgca ggttgtgcca gtggaccagg cagaagcgat cttccggggg
    13141 accgtcaaga gcatttccac ttcggccgta tctcacctgg aagtggagaa gaccatcgaa
    13201 tcgcggctct acgtcaccct cgatatccgt tgcgaagacg ctcgggacgg caccattctc
    13261 tggcaggatc ggaacttcac ttactacaag gtttttctgc aatatctcag ccctaagaac
    13321 ccaaatccca tcatcgggta cgaaagtcga cgggcagctt tggaattcct tgccggcgag
    13381 atgtccgtcc gcatccatga ccgttttcta aacagtttct gaccatgttg ccgggggaac
    13441 ttcaccagcg aatcgaacgg ggggacatcg ctccggtcta cttgttcctg ggcgaagccg
    13501 ccttcttgat ggaggaagcc tgggaacatc tggtgcggac atgtttctcc aaaacggctc
    13561 gccgattcaa gggagagcga tttcttgcca aagagtgtcc tgcgtcggac gtgatcgaaa
    13621 agctttccac cgttcccatg ttcggttccc ggcggctgat cctggtctcc ggcgtcgagg
    13681 catggaacaa ggaccagagg aacgccctga cctcatacct gaaggctcct tcccctcacg
    13741 ggtgcctggt cctgagggcg gctcagagca agggaatcga gtccctggag agttgcgtgg
    13801 cttcggtggg ggccgtcgtg catttcaaga cccccgcgga aaaagatgca ccacggtggc
    13861 ttcgggagcg tgcccagtac cacgggaagc agttgtcccc ggatggagcg atgttcctcg
    13921 ttgaacaggt tggagtggac ctgtttcgct tggaacggga attggagaaa ttggcggcct
    13981 atgtagggag ccgctccagg atcgagctcc gggacgtgca agaggtggtg ggttcccagc
    14041 gctttttcac ggtcttcgaa atgctgcgct tcctgggccg gcgcaaggcc cacaaggccc
    14101 tggtgtctct caggagcctg atccttttgg gggaagcccc cctggcaatt ctgggaatgc
    14161 tggcccggca ggttcgaatt ctgtggcaga tcaaggaagg aatcgaggag ggattgtcca
    14221 cggcggacct ggccaaaaaa ctgaggcttc ctccctacgt ggcgaagaac ctcgccgggg
    14281 aagcgtccct gttttcccat gggaacctga tggagatcca ccaggccctc cgggaagcgg
    14341 acctggcctt gaaaagcacg ggaaccgcac cggagaaaac cctcgagggt ctggtattga
    14401 aattctgcgg aaaaaagccc tcagggtttt ccccctgagg gccggaagtt tcttcttgtg
    14461 ggaggaccca gcccggaaaa gcctcagacg gcttgaagcg agttcacttt tctcgtcagc
    14521 tgggagattt ttctggaagc cttgttctta tgaatcactc catgggcagc cgtcttgtca
    14581 atgatcgaga tcgcttcctt cagtccctcc accgcggcat cgacggcctt ggattgaatt
    14641 gccgtctcga ggctcttgac gacgttcttc atgcgggtct ttcgtgcacg gtttctctgg
    14701 cggcgtgctt cactttgctt ggcgcgcttg atggcggatt tgtgattggc caacgctagg
    14761 tcctcctggt tgctcaaatt ctgcggtttt tcgctgcccc cgatccggga ccggcgggca
    14821 ccatcagagc attattctat agcccgcccg caccgcctgt gtcaaccctt tttgtcataa
    14881 acacaagccg gggagccaac cgccgctttt ggggattttt cgatcgtcca gtcttcgacg
    14941 ccctacttgt tcttgggctg tcgaatggcg gtgaagttga agatctgggc gttgtcgttt
    15001 tttcctacgc ccgccgccat ctgcttggcg tgctcgaggg ttccgtggtt gatggtggcc
    15061 gtgaaaacga tcccttcctc cacgtccttg aacttgaatt ccccgctgag gaggcaattg
    15121 cgactaaggt tcacggcacc tcccacgatg ggggatttgt cgcccatgga gtccgtgcac
    15181 tccccgtcct tgatcttgcc gtctctgccc accaccatct tgcaggcctg ccaccaggga
    15241 ccccattctc cggagcccat ggcgtacacg ttccatact
//
LOCUS       DAOOVC010000095        15264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2601_length_15264_cov_6.723913, whole genome shotgun sequence.
ACCESSION   DAOOVC010000095 DAOOVC010000000
VERSION     DAOOVC010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15264
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_15264_cov_6.723913"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLM79_12045"
     CDS             <1..675
                     /locus_tag="PLM79_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV87087.1"
                     /translation="RVRGYGPSRFSFNVKGGRCEGCKGEGTRIVDMFFLPDVSVTCPL
                     CGGSRYRDETLEVKFKGKSIAEVLQMTVYEASNHFENFPSVRHKLEVIQEVGLGYLRL
                     GQSATTLSGGEAQRVKIAAELGRKSAVDALYLLDEPTTGLHLDDIRRLLHMLQKLVDL
                     GHTAILIEHHPDVVKTADYVVDLGPEGGQQGGNVVAQGTPEEVAQVESSHTARYLRPL
                     LGGRER"
     gene            complement(704..1336)
                     /locus_tag="PLM79_12050"
     CDS             complement(704..1336)
                     /locus_tag="PLM79_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOV87088.1"
                     /translation="MKFAKIPMATINRLSIYMRTLQELLEDEVDVISSERLAKQCGVN
                     PAQIRKDLAYFGEFGVRGVGYRVNDLVNQIREILGLNRPWNLAMVGLGNLGSALIRHG
                     NFTKHGYLFTAAFDVDVHKVGKRLPNGLIINHIDECEEVIKEREVHIGIIASPANAAQ
                     SVANQLVLAGINGILNFAPVQIQVPDCCHVENVDFTIKLDAIAYHLSLGV"
     gene            complement(1537..1908)
                     /gene="atpE"
                     /locus_tag="PLM79_12055"
     CDS             complement(1537..1908)
                     /gene="atpE"
                     /locus_tag="PLM79_12055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006007371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOV87089.1"
                     /translation="MTKKVGLFTTLFVLGLTTLALAADAGASDKAEGLRFFVYSAVAA
                     GLGIAIAAFGCGIGQGMAVKGAVEGIARNPDASGKVTVTMLIGLAMIESLSIYALVVS
                     LILIYANPVAKLIQTFVGLAK"
     gene            complement(2000..2719)
                     /gene="atpB"
                     /locus_tag="PLM79_12060"
     CDS             complement(2000..2719)
                     /gene="atpB"
                     /locus_tag="PLM79_12060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOV87090.1"
                     /translation="MEHPILFLNLILEKLGFHLVGPDEAKSLMDFLLQPHVTYAWVVM
                     ILLLILGKMAAGTLKMVPEKGQNFFEVVITGIEDFMIGVTGEEGRFVFPLIATLGMFI
                     LLSNYLGMIPGFFSPTANINTTAAMALIVVIFTHVIGVKFHGVKYIKHFMGPVWWLTP
                     LIMPIEIIGHLARVLSLSIRLFGNIFGEELVLAILFFLAGMYLAPLPMMLLGLFTGFI
                     QAFIFCLLSMMYFAGAIEHAH"
     gene            complement(2723..3124)
                     /locus_tag="PLM79_12065"
     CDS             complement(2723..3124)
                     /locus_tag="PLM79_12065"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOV87091.1"
                     /translation="MKNGQLEKRLLRRIEGTNAALLVSLPLIAWGLYSKEVALAVLTG
                     GIIATLSFRVLKWQLQRAFLRPGRLPTKGGLFVSYYLRFLGTMLVVFLVMVYGWSKPI
                     PLLVGLSVVMLSIVLVGGREFLAMMLKRGDE"
     gene            complement(3143..3364)
                     /locus_tag="PLM79_12070"
     CDS             complement(3143..3364)
                     /locus_tag="PLM79_12070"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOV87092.1"
                     /translation="MKNETRKYLRQLAMASTLGFQVALSVFIGLAMGVWLDSWLGTFP
                     WLALVFMIFGVAAGFLNYYRFAKKQQQDE"
     gene            complement(3439..4740)
                     /gene="hemL"
                     /locus_tag="PLM79_12075"
     CDS             complement(3439..4740)
                     /gene="hemL"
                     /locus_tag="PLM79_12075"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOV87093.1"
                     /translation="MGWNRSEEFYEEAQKWIPGGVNSPVRSARAVGTHPIFVRKGRGC
                     RLWDEDGNEYIDYVGSWGPLILGHAHPLVVEAVRKAVENGTSYGIPTRLEIEMARKIV
                     EMVPSMEMVRMVNSGTEATMSAVRLARGFTGRKKIIKFNGCYHGHGDSLLVQSGSGVM
                     TLGIPGSPGIPEEIVQHTLSLPYNRLEDLHQAMARIGNQVAAVIVEPVAANMGVVLPH
                     ADFLPGLRKVCDEHGALLIFDEVITGFRLGPGGAQERYGIMPDLTCLGKIIGGGLPVG
                     AYGGRREIMQKMAPAGDVYQAGTLSGNPLAMSAGLATLEVLSRPGVYEELAEKTAYLT
                     SGLEAAANDSGVPVTMNVLESLGSGFFTQGPVVDLETALQANTRAYGVFFREMLKRGI
                     YLAPSQFEAFFVSLAHEKKDLDCTIEAAGPAFMRVREVFGA"
     gene            complement(4897..6462)
                     /locus_tag="PLM79_12080"
     CDS             complement(4897..6462)
                     /locus_tag="PLM79_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOV87094.1"
                     /translation="MSAELIINANFYETRVALVENGQVAELFVERSSDRGIAGNIYKG
                     RVVRVLPGMQAAFVDIDLEKAAFLYVSDVHHPLEDMNRLFLDSCGVEPGEEQPQEPTE
                     PENDELSEVMESVLPFEPVPIEDRLQEGQEILVQVAKEPIGHKGARITTHITLPGRHL
                     VLMPTMDHIGVSRRIENEKERKRLRELMCSIRPPRCGFIVRTAADGSEPEKLQAEMDF
                     LLKLWQNVQRRSEHASVPSLVYQELDVTLRAVRDLFTKEVDRLIIDSEAEYRRVVSFI
                     ETFMPSLKSSVELYQGEEPIFDCYGIEMEVQRALSRKIWLKSGGYIVIEITEALTAID
                     VNTGRYVGKRNLEETILKTNLEAVKEIAYQLRLRNIGGIIIIDFIDMEKEANREKVFN
                     ALREAVRKDKSKTNILHMSELGLIEMTRKRTKENIGRTLCEPCFYCEGEGHLKSTQTV
                     CYEILRELERERPDFFGKSILVTVHPQVAARFCDEESAQLERLEETLHARIVVKGDAT
                     VHLEQYEITPIES"
     gene            complement(6440..8986)
                     /locus_tag="PLM79_12085"
     CDS             complement(6440..8986)
                     /locus_tag="PLM79_12085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOV87095.1"
                     /translation="MPLAERLLFEVLKPGRYLCSEVNAYRKSFEKASTRFVLAFPDVY
                     EVGMSHLGLRLLYHLLNRLEGVVADRVYTPWTDFEGKLRESGEPLRGIESGMPLKAFD
                     FVGFSLQYELSYTNLLTTLDLAGIPAASEDRREGMPWVVGGGPCAYNPEPLARVLDFV
                     VLGEAEEVLPEIIQVFESWKASQGTRTEFLETVAAIPGIYVPSFFDDSYHADGTIRAL
                     RPRHPHLSVLRKRIIRNLDETSPIPDTPLVPMVDIVHNRLSLEIARGCTRGCRFCQAG
                     FIYRPVRERNPGTILQKAEEALARSGYEELSVLSLSTGDYCRIVPLLTGLMDRFAHEK
                     VAVSLPSMRVGTLTPELMGLIRRVRKTGFTLAPEAGSERLRQVINKGISDEDLLSTAR
                     TAFGLGWRLLKLYFMIGLPTETEDDLDAMVRLCLEVWKLAKPSKSSINLSIATFVPKP
                     LTPFQWVGQISREEMEDRLERLKARLRRPGIHIRWNRPDQSVMEAVFARGDRRMGEVL
                     MRAWRSGARFDGWSETFRPEIWERAFEQSGLSPSFYAQRERSPEEILPWDHLSAGVKR
                     DFLWEESLRAGKGEYTPDCRRGRCSSCGVCDHKTVQPELRKEAEPLVLSTQSNAGAQA
                     DEEVVYLLRYSKTGVMRFYGQLEIARSFQRAIRRSGLPAAYSRGFHPHLKLSFAGALP
                     LGLESLVEEACLTLLGHVDPRRVQIDLNRVLPPGLTVEEVAPAPRTGDRPRSRKATYL
                     VEPLSPWEVRSLCQSRAKNPWEILVKKTKRGEIRAKLEEILLDLRPRGDTALEMDLYE
                     GEQVCFRPTAILEQLLREPAQRFMGCLIRKIASSELLDTKGDEDVCRAHH"
     gene            complement(9024..10217)
                     /locus_tag="PLM79_12090"
     CDS             complement(9024..10217)
                     /locus_tag="PLM79_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOV87096.1"
                     /translation="MKSDRIYLDHIAGTPVPPTVVEAMLPFLNEHYGNPASIHHDGEI
                     PQDALFRSREKVAQLIGAQPEEIIFTSCGTESNNLAVKGVSLAGVRQGRHIVVSSIEH
                     YSVLYAAKALERLGFEVTEVPVDRYGQVDPQEVCKALRPDTVLVSVMHANNEVGTIQP
                     LAQIAPHTRERGIVLHADAVASVGRIPVDVEALGVDLLSLSANQFYGPKGVAALYCRK
                     GVRLWPLFHGGGQEDGRRTGTENVPGIVGMGKAAETAVETLSRRMQHCRELETLLRQQ
                     ISERIDDIQFNGHPVEHIPGLVNVSFRYVEGEALLLHMDMRGISVAGASACMSITAKA
                     SHVLEAMGVLGGGALGTLLFSMGEENTRDEIYKTAETLEAVVKMLRAMSPLYNRVGAG
                     TPGKS"
     gene            complement(10340..12937)
                     /locus_tag="PLM79_12095"
     CDS             complement(10340..12937)
                     /locus_tag="PLM79_12095"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOV87097.1"
                     /translation="MAKKKESKKSSKAGLPARSSPPSLVGPSTRRSFWILLGIFFLSG
                     LCGLMYEVVWTRLLVLVFGSTTYAVATVLAVFMLGLSLGSRLFGTLTERFANPQRIYG
                     LLEIAVGLYAFLFLPGIRFVERLHSAAFPYVYDIPHLLNLVRILIAGLAILLPTLLMG
                     GTIPLVTQALAGSRLSVGRDVGRLYALNTLGAASGSFLSAFVIIPHWGLHWTFYIGGV
                     VNIGVGVVAVLLRPVPPALDSEPPGSPFEKGPATSLSGSQSLWALSAFFFTGLLSMVY
                     ENAWTRALVLVFGTSIYAFATMLTTYLLGLGLGSILFGPWADRIRRPFPAYSILLTLV
                     GISVLATTPLLGSLPGFFLGVFGGQDKSWGHITLLEFAVSFLVMLVPTLCNGAGFPLV
                     TRILAHARPIRIGRAAADAYAFNTAGCILGSLATGFLLIPWLGVEQTLLLGGALNLVL
                     GAVLLPAGGNIPKPWVRFLAPAALTGLAVVAFFALPSWDKKVMTSGVYVYSPSMAKAA
                     ASTVESYMSKYNLLFYKDGTSETVAVLESPEGARFLRINGKTDGSDRGDIYTQSLLGI
                     LPALYRPDASDNLVIGLGTGITLGSLLDFSVDRVDVLEISSAVVEASRLFREANGDAL
                     RSPRVHLRILDGRTWLMAMPRRYDIVTSEPSHPWQTGNANLFTLEFFGMVRERLKPGG
                     VVCQWLPYYHMDREHFRILLKTFQTAFPHVHVWVANTDALLIGSAEEPGLSCEDLRSR
                     MKTPGIRDRLGKIGIHDLPDLLSFFYLDTPAVKRFVQGVRQVNSDWFPILEFHAPKYV
                     MGKVKTEIFFDLLNLSYGSKMPLVGDEDLRALHRQRVRQRAKFYEEWYIPDAVIRDMI
                     GKASVYPEG"
     gene            complement(13342..13551)
                     /locus_tag="PLM79_12100"
     CDS             complement(13342..13551)
                     /locus_tag="PLM79_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87098.1"
                     /translation="MRIQKAYFNDKYEASVVCGNCLKKQAINALQFKKHANRIIAKCS
                     CGSIFLVSLRRAGGAPTRMASVLGS"
     gene            14780..>15264
                     /locus_tag="PLM79_12105"
     CDS             14780..>15264
                     /locus_tag="PLM79_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87099.1"
                     /translation="MYLGRILVAGHGGHIADAEIVIDPAASADTIRIPQLHDLDGQQA
                     PFHSPGRCGRLRLPYDVRIDNQDPNTAFWSNYTSKKPSVLVGKLDLRTNRWTAERRLD
                     LPKEVLDFGKTTTKTLYCAPGQTERYFLPIFMGYPGFIDVIDKKDLSLKRRVMMASNP
                     EL"
BASE COUNT         3268 a         4565 c         4434 g         2997 t
ORIGIN      
        1 agggtgaggg gctacggacc cagccgcttt tctttcaacg tcaagggtgg gcgctgcgaa
       61 gggtgcaagg gtgaggggac ccgcatcgtg gacatgtttt ttctgccgga tgtttccgtc
      121 acctgtcccc tgtgcggggg aagccggtac cgggacgaga ccctggaggt gaagttcaag
      181 gggaaatcca ttgcggaagt gctccagatg acggtgtacg aagcctcgaa tcatttcgag
      241 aacttcccgt cggtgcggca caaattggag gtgattcagg aggtgggatt ggggtatctg
      301 cgcctggggc aatcggccac cactttgtcc ggcggggagg cccaacgggt gaagattgcg
      361 gcggaactgg gccggaaatc ggcggtcgac gccctatatc tcctggacga gcccaccacg
      421 gggctccacc tggacgacat ccgaagactg ctccacatgc tccagaagct ggtggatctc
      481 gggcataccg ccatactgat cgagcaccat ccggatgttg tgaagacggc ggactacgtg
      541 gttgatttgg gtcccgaagg ggggcagcag ggagggaacg tagtcgccca aggaactccc
      601 gaggaggtcg cgcaagtgga gtcttctcac acggcccgct atttgagacc tctgttgggc
      661 gggagggaac gatagccccc ctcgggtcct tccccggagc tgatcagacg cccagggaca
      721 ggtgataggc aatggcgtcc agcttgatgg tgaagtccac gttctcgacg tggcagcagt
      781 cgggcacctg gatctgcacg ggggcgaaat tcaggatccc gttgatcccg gccagcacca
      841 gttggttggc cacactctgg gccgcgttgg ccggggaggc gatgattcct atgtgtacct
      901 cccgttcttt gatcacctct tcgcattcgt cgatgtgatt gatgatcaga ccgttgggaa
      961 gccttttccc gaccttgtgg acatccacgt cgaaggcggc cgtgaaaagg tatccgtgct
     1021 tggtgaagtt tccatgtcgt atgagggccg aacccaggtt ccccagaccg accatggcga
     1081 gattccaggg acggttcagc cccaggatct ctcggatttg attgaccaga tcgttgactc
     1141 ggtaaccgac tccccgaacg ccaaattctc cgaaatacgc caggtccttt cgaatctgag
     1201 cgggattgac gccgcattgc ttggcaaggc gctctgagga aattacgtcc acttcatctt
     1261 ccagtagttc ttgcagggta cgcatgtaga tggagagccg attgatcgta gccattggga
     1321 ttttggcaaa tttcatcctc agacctcgac aacgttttca ccggaacggc aggggagatg
     1381 ttgcattttc cggcaaaaac gggctaacta tgtgataaga ttcacattat tccccaaaaa
     1441 aataggcctg gtgaagacct cgcctgcgga ccgggaacca agcccgggga atctgtcccc
     1501 gggcttgggt gttcccgtcc aaactgcgcg ggggtcctac ttggccagac ccacaaacgt
     1561 ctgaatcagc ttggctacag gatttgcgta aatcaggatc agtgagacga ccagggcgta
     1621 aatcgagagg gactcgatca tggccaaacc gatcaacatc gtcaccgtaa cctttccaga
     1681 agcgtcgggg ttgcgcgcga tcccttcgac tgcgcccttc accgccattc cctggccgat
     1741 gccgcatcca aaagccgcaa tggcgatacc cagcccggcg gccaccgccg aatagacgaa
     1801 gaaccgaagt ccttcggcct tgtccgaagc gcctgcatct gcggcaagag ccagcgtggt
     1861 aagacccagt acaaacagcg tcgtgaacaa ccctaccttc ttcgtcataa cccgacctcc
     1921 tttgtatgtg tggaacattg aaaatggttg agtgggaaaa tatgtgaagc ggcctttcct
     1981 ccaaccccgt accgccggtc taatgggcgt gttcgatggc tcccgcaaaa tacatcatgg
     2041 agagcaggca gaagatgaac gcctgaatga acccggtaaa gagacccagc aacatcatgg
     2101 gcaggggggc gaggtacatg cctgccagga agaacagaat cgccaacacc agttcttcgc
     2161 cgaagatgtt tccaaaaaga cggatggaca ggctcagtac ccgggccagg tgcccgatga
     2221 tttcgatggg catgatcagg ggggtcagcc accagaccgg ccccatgaaa tgcttgatgt
     2281 atttgactcc atggaatttc accccgatga cgtgggtgaa gatcaccacg atcagggcca
     2341 tggctgccgt cgtgttgatg ttggcggtgg gggagaaaaa ccccggaatc atccccaggt
     2401 aattgctgag aaggatgaac atgcccaggg tggcaatgag gggaaagaca aagcggccct
     2461 cctcgccggt caccccgatc atgaaatctt cgatccccgt aatcaccacc tcaaagaaat
     2521 tctggccctt ttccgggacc atcttcaggg tgcccgcagc catcttgccc agaatcagca
     2581 acaggatcat cacgacccag gcataagtga cgtggggttg aagcaggaaa tccatcaagc
     2641 tcttcgcttc atcaggtccc accagatgaa accccagttt ttccaagatc agatttaaaa
     2701 acagaattgg gtgttccata ggttactcgt cccccctctt gagcatcatc gccaaaaatt
     2761 cccttcctcc aaccaacaca atactgagca tgaccaccga caatcccacc agcaggggga
     2821 tcggtttcga ccagccgtag accatgacca ggaagaccac cagcatggtt ccaaggaagc
     2881 gcaggtagta ggaaacgaac agccccccct tggtcggcag gcgcccgggt ctcagaaacg
     2941 ccctctggag ctgccatttg aggacccgga agctgagggt tgcaatgatg ccccccgtca
     3001 aaaccgccag ggcgacctcc ttggagtaca acccccatgc aatcaaagga agggacacaa
     3061 gcaaagccgc gtttgtccct tcaatgcgcc gtaaaagcct cttctccagc tgcccgtttt
     3121 tcacaaaacg tctctggtcc gcctattcgt cttgttgttg cttcttggca aaacggtagt
     3181 aattaagaaa tcctgcggcg actccaaaga tcataaagac gagcgcaagc cacgggaaag
     3241 ttcccagcca ggaatccagc cacacgccca tggccaggcc gatgaagacg gataaagcca
     3301 cctggaaccc cagggtgctg gccatggcca gctggcgcaa gtatttccgg gtctcatttt
     3361 tcatggggca acttcgtgta aatgttcaca gagttcctag cacaggggcc gaaccgagtc
     3421 aatgcttttt ttctcgactc aggccccaaa aacctcgcga acccgcataa atgcagggcc
     3481 tgccgcctct attgtgcaat caaggtcctt tttctcgtgg gccaggctca cgaagaaggc
     3541 ctcgaactgg gaaggagcca gatagattcc ccgcttcagc atttcccgga aaaaaacccc
     3601 gtaagctcga gtatttgcct ggagggccgt ctccaggtcc accaccggcc cctgggtgaa
     3661 aaagccgctc cccagggatt ccaggacatt catggttacg gggaccccgg agtcgttggc
     3721 ggcggcctcg aggcccgagg tcagatacgc ggtcttctct gccagctctt cgtagactcc
     3781 cggtcggctc aaaacctcta gggtggccag cccggcactc atggccaagg ggtttcccga
     3841 aagggtcccc gcctggtaga cgtcccctgc gggagccatt ttctgcatga tctcccgcct
     3901 cccgccgtaa gcccctacgg gcaatccccc tccgatgatt tttcccaggc aggtgaggtc
     3961 gggcatgatg ccgtatcgct cctgggcccc gccgggcccc aatcggaatc ccgtgatgac
     4021 ttcatcgaag atgaggagcg ctccgtgctc gtcgcagacc ttccgtaacc ccgggaggaa
     4081 atccgcatga ggcaacacca ctcccatatt agcggcaacc ggctccacaa tgacggccgc
     4141 cacctggttt cctatgcgcg ccatcgcctg gtgcaggtcc tcgagacggt tgtagggcaa
     4201 ggaaagggta tgctgtacga tctcttccgg aatgccggga ctgccgggaa tccccaaggt
     4261 catcaccccc gatcccgact ggaccagcag actgtccccg tgaccgtgat agcagccgtt
     4321 gaacttgatg atcttctttc gaccggtgaa gcctcgggcc agccgaacgg cactcatggt
     4381 ggcctcggtt cccgaattga ccattcgcac catttccatg ctgggcacca tttccacaat
     4441 tttgcgggcc atttcgattt ccagtcgggt gggaatgccg taggacgtgc cgttctccac
     4501 ggccttgcgg acggcctcga ccaccagggg gtgggcatgg cccaaaatca gcggccccca
     4561 ggacccgaca taatcgatgt actcattgcc gtcttcatcc cagagacggc acccccggcc
     4621 cttgcggacg aagatcggat gggtccccac ggcccgtgcg gaccgcaccg ggctgttcac
     4681 ccctccgggt atccactttt gagcttcttc atagaactcc tcggaacgat tccatcccat
     4741 ggcttcatcc tctctagggt cggactcgcg cgtaggcccg aagctcctgc cgggccgttg
     4801 cggcatcgat gaacatttca gggtcttggg tcgcccgagc cgggagcagg gggaaaggaa
     4861 cggagcctcg tctgcctggg cgcaccaacc atgccctcag gattcgatgg gagtgatctc
     4921 gtactgctcc aggtggaccg tcgcatcccc tttgacaacg atccgcgcat ggagggtttc
     4981 ctcgagccgc tccaattgtg cgctctcttc gtcacagaaa cgtgcggcca cttggggatg
     5041 aacggtcaca agaatgcttt ttccaaagaa atcgggacgt tctctttcca gttctctcag
     5101 gatctcgtag cagaccgtct gggtagactt gagatgtccc tccccctcac agtagaagca
     5161 cggttcgcaa agagtgcgcc cgatgttttc cttggtgcgt tttcgagtca tctcgataag
     5221 ccccagctcc gacatgtgga ggatgttggt cttgctcttg tcttttcgca ccgcctctct
     5281 gagggcgttg aacactttct cccggttggc ttccttttcc atgtcgatga aatcgatgat
     5341 gatgataccc ccgatattgc gcaatcgcag ctgatacgcg atctccttca ccgcttcgag
     5401 gttggtcttg aggatcgtct cttcgagatt cctcttgccc acatatcgac cggtgttcac
     5461 gtcgatggcc gtgagggcct cggtgatttc gatgacgatg tatccccccg acttgagcca
     5521 tatcttccgg ctcagggccc tctgcacctc catctcgatg ccgtagcagt cgaagatggg
     5581 ctcttctccc tggtaaagct ccacggagct cttgagggaa ggcatgaagg tctcgatgaa
     5641 gctgaccacc ctgcgatact cggcctcgga atcgatgatc agtcggtcga cctccttggt
     5701 gaaaagatcc cttacggcgc gcaacgttac gtccagttcc tggtagacca gggatggcac
     5761 ggatgcgtgt tcggagcggc gctgcacgtt ttgccagagc ttcaggagga aatccatttc
     5821 cgcctggagc ttttcaggct cggagccgtc ggcggcggtt cggacgatga agccgcaacg
     5881 agggggacga atggaacaca tgagctccct tagccgtttg cgttccttct cgttttcgat
     5941 tcgccgggag actccgatgt gatccatggt gggcatcagg accaggtgtc tgccgggaag
     6001 ggtgatgtga gtggtgatcc gggctccctt gtggccgatg ggctccttgg cgacctgcac
     6061 gaggatttcc tggccttcct ggagtcgatc ttcgatgggg acgggctcga aggggagcac
     6121 cgattccatg acctcgctca attcgtcgtt ttcgggctcc gtgggttcct gaggctgttc
     6181 ttcccccggt tccactccgc aggaatccag gaacaaccgg ttcatgtctt ccagagggtg
     6241 gtggacgtcg ctcacgtaca ggaaagcggc cttttccagg tcgatgtcca cgaaggcagc
     6301 ctgcatgccg ggcaacacac gaacgacccg gcccttgtag atattgcctg caatgccgcg
     6361 atcggagctt ctttccacga aaagctcggc gacctgtccg ttctcgacca gcgccacgcg
     6421 cgtctcatag aagttggcat taatgatgag ctctgcagac atcctcatct ccctttgtat
     6481 cgagcaattc gctcgaggca atcttgcgaa tgagacagcc catgaacctc tgggcgggtt
     6541 ccctcagaag ttgctccagg atggcggtgg gacgaaaaca tacctgctcc ccttcgtaaa
     6601 gatccatctc caaggccgta tccccccggg gacgcaggtc gaggagaatc tcttccagct
     6661 tcgcccggat ctcccccctc ttggtcttct tgaccaggat ctcccaaggg ttcttggccc
     6721 gggactgaca caggctcctc acctcccacg ggctcaaggg ctccacgaga taggtcgcct
     6781 tccgggacct gggcctgtcc cccgttcgag gggccggagc gacctcttcc accgtcagtc
     6841 ccggcgggag gacacgattc aggtcgattt gaacccgtcg gggatccacg tgtcccagaa
     6901 gggtcaggca ggcctcttcc accaggcttt cgaggcccag gggaagggcc cccgcaaagg
     6961 agagcttcaa atggggatga aaccctcgac tgtaggccgc aggcaggccg ctcctgcgaa
     7021 tggcccgctg gaagctccgg gcaatctcca gctgcccgta aaaccgcatc acgcctgtct
     7081 tgctgtatcg aaggaggtag acgacttcct catccgcctg agcccccgca ttggattggg
     7141 tggaaagaac gagaggttcg gcttccttcc ggagttccgg ctgaaccgtt ttgtgatcgc
     7201 acactccaca agaactgcat cggccccggc ggcagtcggg agtatactct cccttgcctg
     7261 cccgcaggga ttcctcccac agaaaatccc gcttgacccc tgcggaaagg tgatcccagg
     7321 gcaggatctc ctccggagac ctttctcgtt gggcgtaaaa ggaaggggac aatccgctct
     7381 gttcaaaggc ccgttcccag atttcgggtc ggaaggtctc ggaccagccg tcaaaacggg
     7441 caccggatct ccatgcccgc atcaaaacct cccccattct gcggtcccca cgggcgaaca
     7501 cggcttccat cacgctttga tccggccggt tccaacgaat gtggatgccc ggccggcgca
     7561 atcgagcctt tagccgttcc agccggtcct ccatctcctc ccgggagatc tgtcccaccc
     7621 attggaacgg agtgaggggt ttggggacaa aagtcgcaat ggacaagttg atggaggact
     7681 tggaaggttt cgccagtttc cagacctcca ggcacaggcg caccattgca tccagatcgt
     7741 cttcggtctc cgtggggagg ccgatcatga aataaagctt caacaggcgc catcccagtc
     7801 cgaaggccgt gcgggccgtg gacagaaggt cctcgtcgga aatccccttg ttgatcacct
     7861 gcctcagtcg ctcactgccc gcttcggggg ccaaggtgaa ccccgtcttg cggacccgcc
     7921 ggatcagtcc cataagctcc ggcgtcaagg tccccaccct catggacggc agggaaaccg
     7981 ccaccttctc gtgggcgaaa cggtccatga ggccggtgag caggggaaca atccggcagt
     8041 agtcccccgt gctcagagac aacaccgaca gctcttcata cccgctgcgg gccagggctt
     8101 cttccgcctt ttgcaggatg gtgccggggt tcctctctcg gaccggtcga tagatgaacc
     8161 ccgcctggca gaagcggcat ccgcgagtac atccccgagc gatctcgagg ctgagccgat
     8221 tgtgaacaat gtccaccatg ggaaccagag gcgtatcggg gatgggggag gtctcatcga
     8281 ggttccgtat gattctcttt cgaagtacgg acagatgggg atggcggggc cggagggctc
     8341 gaatcgttcc gtcggcgtga taggagtcgt cgaaaaagga ggggacgtag atccccggga
     8401 tggcggcgac ggtctcgagg aattccgttc gggttccctg agaggccttc caggactcga
     8461 aaacctggat gatttccggc aggacttcct cggcctcgcc caacacaacg aaatcaagga
     8521 cccgagccag gggttcggga ttgtaagcac aaggcccgcc ccccaccacc cacggcattc
     8581 cctcccgtcg gtcctctgat gccgcgggaa tgcccgccag gtccagggtg gtcaggagat
     8641 tggtgtagct gagttcgtac tggagactga atccgacgaa gtcgaaagcc ttcaggggca
     8701 tcccggactc gattccgcgc aggggttccc ccgattcccg gagtttgccc tcaaagtcgg
     8761 tccagggggt atagacccgg tcggcaacaa ctccctccag ccggttcaag agatggtaca
     8821 ggagacggag tcccaggtgg ctcatcccga cttcatacac atcgggaaag gccagcacaa
     8881 agcgggtcga agctttctca aaactcttcc gataggcatt cacctcgctg cagaggtaac
     8941 ggccgggctt gagtacctca aacaggagcc tttccgctaa gggcaagtct tttctcgggg
     9001 aaggggtcat tccataccgc ccatcatgat ttcccggggg tgcccgcccc gactcggttg
     9061 tacaaagggg acatggctcg caacatcttc acgacggcct cgagggtctc cgcagtcttg
     9121 tagatctcgt cccgggtgtt ttcctccccc atgctgaaca gaagggttcc caaagccccg
     9181 ccccccagga ctcccatggc ttcgaggaca tgggacgcct tggcggtgat ggacatgcag
     9241 gccgaggctc ccgcgacgct gatgccgcgc atgtccatgt gtaacaacag ggcctctcct
     9301 tccacgtagc ggaacgaaac gttgaccagg ccggggatat gttccacagg gtgtccgttg
     9361 aactgaatgt cgtcgatacg ctcggagatc tgttggcgaa gcagggtctc caactcccgg
     9421 caatgctgca tccgtcgaga cagggtctcg acggccgtct cggccgcctt ccccatgccc
     9481 acgatccccg ggacgttttc cgttcccgtt cggcgcccgt cctcctggcc tccgccgtgg
     9541 aagaggggcc acaggcgcac gcccttgcgg cagtaaaggg cggcaactcc tttgggaccg
     9601 tagaactggt tggcggaaag actcagcaga tcaactccca acgcttccac atccacggga
     9661 attcgtccca cggaagcgac ggcgtcggcg tggagaacaa tcccccgttc ccgagtgtgc
     9721 ggggcaatct gcgccaacgg ttggatcgtt cccacctcgt tattggcgtg catgacggac
     9781 accaggaccg tgtccgggcg cagggccttg cacacttcct ggggatccac ctgcccgtag
     9841 cggtccacgg ggacctccgt gacctcgaag cccagccgtt cgagggcctt tgcggcgtac
     9901 aagaccgaat agtgctcgat ggaagacacc acgatgtgcc tgccttgccg aaccccggcc
     9961 aacgacactc cctttacggc caggttgttg gattcggtcc cgcaggaagt gaagatgatc
    10021 tcttcgggtt gggcgccgat gagctgcgcc accttctccc ggctcctgaa gagggcgtcc
    10081 tgagggattt ccccgtcgtg atggatgctt gccggatttc catagtgttc gttgaggaaa
    10141 gggagcatgg cttccaccac agtaggagga acgggggtac ccgcaatgtg atccagatag
    10201 atccggtcgc ttttcattgc tgcagcctcc acacgcatcg tctgtcggca aacccggaaa
    10261 aggaaaagcc gaacgttaca acatacttat tcatccaaat cggagtcaat caaaatcacc
    10321 gggacaacgg gctcgatccc tatccctcgg ggtagacgct ggccttccca atcatgtccc
    10381 ggattaccgc gtccgggatg taccactctt cataaaactt cgcccgttgc ctcaccctct
    10441 gccggtggag agccctcagg tcttcatccc ccaccagagg catcttggag ccgtaggaca
    10501 ggttcaacag atcaaagaaa atctcggttt ttaccttccc cattacatac ttgggcgcat
    10561 ggaactccag gatgggaaac cagtccgaat tcacctgtcg gactccctgc acgaaccttt
    10621 tcaccgcagg ggtatcaaga tagaaaaagc tcaacaggtc cggcaggtcg tgaatcccga
    10681 tctttcccag ccggtcccga atgccgggcg tcttcatgcg gctgcgcaaa tcctcgcaag
    10741 acagcccggg ttcctcggca gacccaatga gcagcgcatc cgtgttggca acccacacat
    10801 gcacgtgggg aaaagccgtc tggaaggtct tgagcaggat gcggaagtgt tcccggtcca
    10861 tgtgatagta cggaagccat tggcacacga cccccccggg cttcaatcgt tctcgaacca
    10921 tgccaaaaaa ttccagggtg aagagattgg cattccccgt ttgccacgga tgggaaggtt
    10981 ccgaggtcac gatgtcgtag cgccggggca tggccatgag ccaggtcctc ccatccagga
    11041 tccgcaggtg cacccgggga gaacgcaagg cgtccccgtt tgcctcgcga aagagccggg
    11101 acgcctccac caccgcggag gagatttcca gaacgtccac acgatccacc gaaaagtcca
    11161 ggagacttcc cagggttatt cccgttccca ggccgatcac caggttgtcg gaggcgtcgg
    11221 gacgatagag ggcgggcaaa attcccagga ggctctgggt gtagatgtcg ccccggtccg
    11281 atccgtcggt cttcccgttg atccggagaa agcgggctcc ttcgggtgac tccaacaccg
    11341 ccacggtctc cgaggttccg tccttgtaga ataaaaggtt gtacttggac atgtacgatt
    11401 ccacggtgga ggcggccgct ttggccatgg acggggaata gacgtacacc ccggaggtca
    11461 tgaccttctt gtcccaggac ggaagtgcaa agaaagcaac cacggcgagg cccgtcagag
    11521 ccgccggagc gaggaacctg acccacggct tgggaatatt gccgcccgcc ggcagcaaga
    11581 cggcccccag caccaggttc agtgctccgc ccaagagaag ggtctgctcc acccccagcc
    11641 atgggattag gaggaacccc gtagccagag atccgagtat gcagcctgcc gtgttgaaag
    11701 cgtaggcgtc ggccgccgcc cggccgattc ggatgggtcg agcgtgggca aggatgcggg
    11761 tcaccagcgg gaaacctgcg ccgttacaaa gagtggggac aagcatcacc agaaaggaga
    11821 cggcgaattc cagcagagtg atgtgtcccc agctcttgtc ctgccccccg aagaccccca
    11881 ggaagaaacc cggaagggaa cccaggaggg gagtcgttgc caggaccgag attcccacca
    11941 gggtcaggag gatggaatag gccgggaagg gcctcctgat tcggtcggcc cacggcccga
    12001 agaggatgct ccccagtccc agccccagaa gataggtggt cagcatggtg gcgaaggcat
    12061 agatggaggt tccgaaaacc aggaccagcg cccgggtcca ggcgttctca taaaccatgg
    12121 aaagaagccc ggtaaagaag aaggcggaca aagcccacag ggattgggac ccggaaagag
    12181 aggttgccgg ccccttctcg aagggagacc ccgggggctc cgagtcgaga gccgggggga
    12241 ccggtctgag caggacggct accactccca ccccgatgtt cacgactccc ccgatgtaaa
    12301 acgtccagtg gaggccccag tgagggatga tgacaaatgc gctgagaaag gaacccgagg
    12361 cggctcccag cgtattcaaa gcgtagagcc ttccaacgtc tctgccgacg gagagtcgag
    12421 atccggcaag ggcctgggtc acgaggggaa tggtccctcc catcaggagg gtcggaagca
    12481 ggatggcaag gcccgcaatg agaatcctca cgagattcaa cagatgggga atgtcgtaaa
    12541 cgtaaggaaa tgccgcagag tgaagccgtt ccacgaaccg gatgccgggt agaaacagga
    12601 aggcatatag cccgacggca atttccagca gcccatagat ccgttgggga ttggcaaaac
    12661 gctcggtcag agtcccgaac aggcggcttc ccagagagag tcccagcatg aataccgcca
    12721 agaccgtggc tacggcgtag gtggtgctgc cgaagacgag aaccaggaga cgggtccaga
    12781 cgacctcata catcagtccg cagagtccgg acaaaaaaaa gatccccagc aggatccaaa
    12841 aacttcgccg ggtcgacggc ccgacaaggc tgggcgggga ggaccgagcc ggtaaaccgg
    12901 ccttcgagga tttcttggat tctttcttct tggccatcct cgtcacacgt ccttgctgag
    12961 atctcgtttg cccgattccg agcgtgccgc agggagtgct cgaaacgcgc ttcgggcaag
    13021 ggccgccccg aaaaagggtc ggaaacgcgt tacctttgta gcgcctccgg ttaagagtgt
    13081 aaagatattc tctggggcac cgcgggcaaa gaaagcccgg agcttgccgc cccaaactcc
    13141 ctgtctgctt ctcacaggcg gtcaagggag gccggatcaa aggaaggacc tctccccggg
    13201 gacggggaga ggctttgatg agaaacaacc tggtaggctg aggattggcg agcaaaaagg
    13261 tcgaacgtaa aatgtaatgt tacagatctg tctccttgag gcgggttccg gatcgaatgg
    13321 gacgatctcg tgggttcgcc tttaggaccc caaaaccgag gccatccggg tgggggcgcc
    13381 gccggccctt cggagggaca ccaggaaaat ggaaccgcag ctgcacttgg cgatgatgcg
    13441 gtttgcatgt ttcttaaact gtaaggcgtt gatggcctgc ttcttgagac aattgccgca
    13501 caccacggag gcctcatact tgtcgttgaa atatgccttc tggattcgca tttccaccta
    13561 tctcctcgat gaacttccct gacacaacgc gtacaaagcc ggcttgtttc ccggcggccg
    13621 ggaaaaccac ccaagccgcc gtgtccccgt ggttcctggg gacctgctct ttccggtgga
    13681 gccatgtcgc tcccatggag gggcggggac ccttccttcc ggcgcgggca ctctgaaagt
    13741 cgcacgggcg tatcctggga ttagcaatat tgatgccaga tcatgacggg cgggggaccg
    13801 tccccgggag gaaacgcggg tttcgcgtcc cttgaaggac ccggtccaaa gggccttgcc
    13861 gggaggagac tctcgggcgg tgaacttctt gtccggaatt ccgcacggtt ttcaagagtt
    13921 gcaggacaac cggaagatcc tccgtggaaa ggctcaggag gttcctgatg caaaaatcag
    13981 gaaactcctg atggacatcc cgtggaggcc atgctttcat gaggccagct tagagagagg
    14041 attacaggtc gagaaggagc attgccgctt ggccttcaca atcctctctc tctattttga
    14101 tttccctcgg gcgaccgcga tccccatccc atccttcccg ccgagggaaa acgccgcggt
    14161 tgttctcaac cggcccgggt tcccccctat gcctgctcac tgcaaatgca ggacgctcaa
    14221 agagaaacaa gatgctccga accttgatcg tttcccatgg gatacctcgg gaccttcgag
    14281 ggggcgtgag caggggggga gaccctcttt ccatggtggg gggaggacac cggccccggg
    14341 tttggggcgg ggcagtgagc ttctcttggc ggccgtgagt aaagaaacag gatcggtatc
    14401 tgtacggaat gacttggcat tctagtaggt gaaatcatga acggggacac ctacctttgg
    14461 tattgataga atacctacaa atgaccgccg gtcccgtcga tgccgagagc aattcaaggc
    14521 gccggtcgac gggaaaagaa cgaaagcggg agcatctccc ccgatcaagg ctgccgtgcc
    14581 cttccccatt ccatccgcct cacggggttt ccatgggcaa accacccttc ggcagtcatc
    14641 ccagggcgtc caaagcggcg tttcgccaat cacggagatc tttatgcaga gaaacggaat
    14701 caagtgggtc ctcttccttc tgtcgggagc cgtcaccctt gcggtctagg ccggactgct
    14761 cagcggaagc caaacgggca tgtacctggg aagaatcctc gtggcgggtc acggcgggca
    14821 tatcgcggat gccgagatcg tcatcgatcc cgcggcctcc gccgacacca tccgaatccc
    14881 ccaactacac gatctggacg ggcaacaagc tccatttcat tcccctggga gatgcggccg
    14941 actacgcctc ccctacgacg tgagaatcga caaccaggac cccaacacgg ccttctggtc
    15001 caactacacc tccaagaagc cgtcggtcct ggtgggaaaa ctggacctgc ggaccaaccg
    15061 atggacggct gaaagaaggt tggaccttcc caaagaggtc ctggactttg gaaagacaac
    15121 caccaaaacc ctctactgcg ccccgggaca aaccgagagg tatttcctcc ccatcttcat
    15181 ggggtacccg ggtttcatcg acgtcatcga caaaaaggat ctgagtctca aacgtcgggt
    15241 catgatggca agcaacccgg agct
//
LOCUS       DAOOVC010000096        15049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2654_length_15049_cov_5.752634, whole genome shotgun sequence.
ACCESSION   DAOOVC010000096 DAOOVC010000000
VERSION     DAOOVC010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15049
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2654_length_15049_cov_5.752634"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            974..1516
                     /locus_tag="PLM79_12110"
     CDS             974..1516
                     /locus_tag="PLM79_12110"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOV87100.1"
                     /translation="MTRRELLKMALLGVQPFITFPVEEVAAYVAGKRYSGELSFHNRH
                     TGETLTTRYIGKNGLFDADGYAKLNRFFRCIHTNETIPINPNLFLLLDTLRCRLGARE
                     RPFLLYSGYRCPSFNRQLMREDSGVARNSYHMRGMAADVCMDGVSLKDMKRTAKTLKG
                     GGVGAYRDFVHVDVGPVRSW"
     gene            1769..2398
                     /locus_tag="PLM79_12115"
     CDS             1769..2398
                     /locus_tag="PLM79_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87101.1"
                     /translation="MRGKTAFFPVLLWLMIWAAIASAASSKAIDESNEYGGITEERTF
                     SAGDAAFDVDGIQKLVSYYDGNGNVRVRETHFTEASSGKDGVEKLVRRFSDRGSPASD
                     EYDFRDDSRIAKGVARLVRHYGEDGRKTHEEYFYTDTSSRKDNVFRMVRYFDAKGKKI
                     RDELHSTGTYAAMTGFSKLVRAYDGDENPAGVQVYDRDGKIVEEEPKSR"
     gene            3062..3187
                     /locus_tag="PLM79_12120"
     CDS             3062..3187
                     /locus_tag="PLM79_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87102.1"
                     /translation="MGRLCSNPLSAVLAPAFLVLALTGCKSGGRVRMRFLEETRK"
     gene            3225..5576
                     /locus_tag="PLM79_12125"
     CDS             3225..5576
                     /locus_tag="PLM79_12125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOV87103.1"
                     /translation="MDNVSPTYTWEWEGVISGLDCADCAATLARDIEKMEGVKTASLN
                     FATSRLRIEYDPASFTLKRMQKELRRAGYGLARQDEFQRAVVMIGGMDCADESTIIEK
                     QLKRLAGVGDLKFNLVAREVVIEYDPSRVKLEDIIRAIDATGMKGRLGGSELAAESFW
                     ERHKHVVLTCLSGAFILAAFLFSWAGYPHAVTDPLYVLAMVTGGWFIARKGLMAVRTL
                     SLDMNFLMMTAAIGAGVIEEWLEGASILFLFSVANILQNLTMNRARKAIRSLMELSPS
                     EVIVRRNGRDERVPVDAVRIRETIVIRPGEKIGLDGRVVEGSSYVNQAPITGESMAVE
                     KNPGDAVYAGTINQNGSLEVEVTHDHKDTTLSRIIHMVEEAQAQKAPSQGFVEEFAKY
                     YTPAVIGAAVLVATVPPLAFGLPFDAWFYRALVLLVVSCPCALVISTPVSIVSGLTVA
                     ARNGVLIKGGVYLEKAGSLKTIAFDKTGTLTGGVPVVTDVVALNGHSEADVLTLAAAV
                     ECRSEHQIGAAIVREAAKRGISCAVPADFQAMTGKGVKARLNGRTLYIGNHRLIKELG
                     KCSAVIDAKMLGFESERKTAVIVADEDQAVGIIAISDEVRAESRAAIEELKASGIEKI
                     VMLTGDNHGTARAIAESLDVDEVHAEMMPEDKATIISKLMREHGKVAMVGDGVNDAPP
                     MALATIGIAMGAIGTDTALETADIALMTDDLSRLPFLVRLSRKTVGTIKQNVVFSILV
                     KAVFIILAVSGLATLWMAVGADMGASLLVIFNGLRLLAVKGKE"
     gene            5697..7268
                     /locus_tag="PLM79_12130"
     CDS             5697..7268
                     /locus_tag="PLM79_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HOV87104.1"
                     /translation="MIGKWRKSLENRRYGLKTVTAVAMSFLTAGVLLTAGFNWTGPSS
                     AQNSEAEKHVSAEPSAPVPAPSLAPLFGQPFGASFAPLTEKLTLSVVNVKVTKIENAA
                     IHGPQGPGEEPYDPYDEFFGNGDRFHRNEGRFPGNGGNQLVQGAGSGVIISSDGSILT
                     NNHVVEGAREVRVILGDKRELSARVVGRDPKTDLAVLKVAAGKDLPAAAMGDSESLKV
                     GDWVLAIGNPFGLGETVTSGIVSAKGRVIGAGPYDDFIQTDASINPGNSGGPLFNMKG
                     EVVGINTAVMAHGQGIGFAIPINTAKPLIPQLVSKGKVTRGYLGVNIQSITPEIASAL
                     KLGERKGALVADVFDGSRAERAGIEHGDLIVAYNGKEVKSSQDLATMVASSSVGEEVT
                     VTVLRHDNEKELRIRIARLQPDRADLEELEGQFQEQLGMMLEDVTPRVAREHRIRLEY
                     GAYVADVQPGGPADEAGIQEGDVIVEVNHQPVKSVDEVEEQVAGADDQAPVLFLVHRG
                     RGMIYIALSNRAQNP"
     gene            7265..7714
                     /locus_tag="PLM79_12135"
     CDS             7265..7714
                     /locus_tag="PLM79_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87105.1"
                     /translation="MIQLHSGCPGVRLLVVEDEKKVACFIKKCLEEEGFSVDVALDGE
                     TGIRLATAEAHDVIILDFRLLQMSGIEVVGALRRNGSAASVLLLSANPTVEEIVLGLD
                     SGADDFLAKPFVSRELAARIQTLVRRRAQVQRAHLRASPGCHQESEK"
     gene            complement(7739..8632)
                     /locus_tag="PLM79_12140"
     CDS             complement(7739..8632)
                     /locus_tag="PLM79_12140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOV87106.1"
                     /translation="MAKQLSAMGQHRRSLWVTFAFTFIYFLVELIGGILTNSLALLAD
                     AAHMMTDVGGLALALFASWIASKPATPSKTYGYYRVEILAALLNGVILFLLSFYIVYE
                     AYRRFQSPPEVASLPMLAVAVVGLAVNLIGIWNLRGGSKESLNVQGAFLEVVSDTLGS
                     VGVIVAAGIMAATGWYYADPIFGVAIGLFILPRTWKLMSQAVHILLEGTPSHINPKAV
                     EDAMCAEPGVLTVHHLHVWTITSGIEALSGHVVLVEGTKADAAQRILENLNQRLKNEF
                     GIDHTTLQVEYTDLVSGENGP"
     gene            complement(8713..9765)
                     /locus_tag="PLM79_12145"
     CDS             complement(8713..9765)
                     /locus_tag="PLM79_12145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOV87107.1"
                     /translation="MRSGAPVAIATAVLFGASTPFSKILLGRVDPMLLAGLLYLGSGL
                     GLTLWRLVRMGFQKRGNGEASLRAADLPWLGGAILAGGVAAPVLLMIGLKATSASSTS
                     LLLNLEGVFTATLAWFVFKENFDRRILLGMVVITLGCVVLSWSGEEPFFISWGSLAVI
                     GACFSWGIDNNLTRKVSEGEPLEIAAAKGLIAGAVNTAIALASGAALPAPWPLFASAC
                     VGFLGYGISLMFFVLSLRHLGTARTGAYFSLAPFVGAFIAILILGEGVTLSFLAGAVL
                     MGIGVWLHLTECHEHEHQHDAFEHNHSHVHDEHHRHHADCLLQDDTAHSHVHAHCEVL
                     HHHPHYPDIHHRHEHC"
     gene            complement(9978..10424)
                     /locus_tag="PLM79_12150"
     CDS             complement(9978..10424)
                     /locus_tag="PLM79_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOV87108.1"
                     /translation="MNEMLLCGGYPALFFLSFAASTLLPVGSEWLLVALILKGFSPAM
                     TVAVATFGNYLGACSTFWIGSYGSPILIRKVLRIDEASQERASRYYNRYGVWSLFFSW
                     LPIVGDALCLAGWLFGVGFLRFSLLVFGGKLVRYASVAVITHVASG"
     gene            complement(11024..11836)
                     /locus_tag="PLM79_12155"
     CDS             complement(11024..11836)
                     /locus_tag="PLM79_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87109.1"
                     /translation="MKGKIVTEALLEMYYFQAIRDHFASAFGARVFRILKPSQRQEGW
                     VGFDQGWVLTELSNEQFYSELETALPSGATSLSRFYFGFFLQFKVVEIMNRRSRYSPR
                     GFTADYFRSELDLTRSKESGAIQHHLLIALSGINGASVAYACPMFFNIDAIYDPPDLN
                     HLRCVPVTDRTPPFDEGERHFIAIQNPTDNWGFWCSQPVYMRHVSFTDWVSKNTENGP
                     KPMDGMQLRDLIESSASVAREHAFWLDSDERKDPSSLLPRSFTVVEMRATEP"
     gene            complement(11971..13059)
                     /locus_tag="PLM79_12160"
     CDS             complement(11971..13059)
                     /locus_tag="PLM79_12160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOV87110.1"
                     /translation="MKPIEKKKGNSLEFLTASYFQAHGYLVRRSVKLSVAAGTAEATD
                     IDLLAIRFNTPLAEERVVVDCKDRKKSHPFERILWTLGLSSFSRAQRAVVVTPKVPWQ
                     AREFAAQGAVEILDALDLETFLDSAGGKIQAFGEADPEVANQFEVCKKHMGEENKHLL
                     REDLKLRQMLTIGSPLTNFNRVVATFSMIKKQPAGIRDDVAWISRYICFNAAVVAAVM
                     LVRFSAESKWTPEKDWSAYARKKLTYGDTSPVKAQQLAKLAFGTDFFEGLPPPHYTDE
                     IIEVIKALISRPDIAVQVPYALDYHLLGCVLGKTPKDAVHPVLGARQEEVLKMGKRIL
                     SALAYAAEMDVSIWEDENTSTVPKDRAR"
     gene            complement(13056..14357)
                     /locus_tag="PLM79_12165"
     CDS             complement(13056..14357)
                     /locus_tag="PLM79_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87111.1"
                     /translation="MSIAGTKPAMTKSRMGAWIINTSKHLYNFDNTNPSFTSLENLFF
                     AGKCGSLLVKISADATEQLSDTKVKAHARLCGISGPELRIYLDTLKASGCIDWDNTHS
                     VYEVLAFTRQRVLETASQILLGAVGITPIEQVLPEFLEYCLVRPRLEAEAKAFLSERL
                     KEKDVNHLLGLVANFELLGVLPIRSKSDRLFFNSYQFGNRAEDIGNALSALSTELREK
                     LNELLEKVASRPGIPPEDTGIPVEIRKMAIGLGLVEVSKVPSSAGDAKFLTLPRLAPP
                     SVGVETERLEEDVFHHAKILLSSLRFGELRSSLSRGRIIDPPVLVNALLQRDRVGPCT
                     AIGQDYTLPEGEGIIRTVRASHKPGNQFYMELRRREPAEIVLNILESGSVSTVSGSML
                     QQSLELPTGFVGPEASRVPARSRLVKNDPETMKRFLEALRT"
     gene            14736..>15049
                     /locus_tag="PLM79_12170"
     CDS             14736..>15049
                     /locus_tag="PLM79_12170"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOV87112.1"
                     /translation="MYPTRLVERLTGATANQLKYWARTGLVCPAREGKCFFFSFKEIV
                     KLRVLVSLRKKGLSLQKVRVGIENLASILPDAEPLTRLIIYTDGVDMIVAEKGNYFSA
                     IT"
BASE COUNT         3431 a         4109 c         4312 g         3197 t
ORIGIN      
        1 cgaccggcct gtcgaacgct ttcactcgat caccgcgacc ctcgagaagg attttgagcg
       61 tcctcagcaa tgaggcatca ttttcgacga ttacaatctc catactcaac actctgttca
      121 aatggctctc caccttgcgg caaaattcca tacggaaaat gatcggattc aatccgtggt
      181 agccagtgtt tctggcttaa gatatagcaa aaagagtgcc attcgaagaa gtgccttgct
      241 ggaacgcgat gttcagggat tgaacctcgt gtctcatcat acttcggaca ggacgagcgg
      301 ttcgcaatga gggcatgtcg gcagaatccc ttgaacgaag ccttcagtcg gcaaggatcg
      361 tgcattgatc accacccgtt ccgtgggctg aggcaagcag ggtgtgtgaa tagactcgaa
      421 cctggtgaaa cactggcaga ctggatgaaa gaaagaagtt acaggcagag cgggacttgc
      481 tcccggatag tctctcgatt ggcagtcctt ctggttcttc tcatcgcctg tctcgtgcac
      541 atctcgcatg cctgcacccc aaagaatgcc ttactgccgc gaaggtctcc cacccgcgtt
      601 caggctttca tgcagggtcc acccccagat gccggggggg cgtggctcgg gatgtctttt
      661 gaacgcgaac aaccgccact actcccgacc gccctgttca ctctcatgcc aacagacccc
      721 ttttttccgg gccgattgtg cccgggagag tctgaaagcc accaacagga atatcttgat
      781 gcgcatgtcc aggtgcgcgc ccctccgctt acatgcctct gtacccgatc tgtatgaatt
      841 aaacccatta agaccataat ctttttgttt gcttgcctga aagagaggag aagctacaga
      901 tccgccgccc tctaccgacg tgaggcaacg acaccaaaaa gtgtccaggc aagccaatgt
      961 aggaggtaag cgagtgacga gaagagagct tttgaagatg gctctccttg gggtgcagcc
     1021 cttcattacg tttcccgtcg aggaagttgc cgcgtacgtg gcgggtaaac gatattcagg
     1081 agagctctct ttccacaatc ggcacacggg agaaactctc acaacccgat acatcggcaa
     1141 gaacgggctt tttgatgccg atggctatgc gaagctcaac cgcttctttc ggtgtatcca
     1201 tacaaacgaa accattccca tcaacccgaa cctgttttta ctcctggata cgctccgctg
     1261 ccggctgggg gcacgagaac ggcccttttt gctctactcg ggttatcgct gtcccagctt
     1321 caacaggcag ctcatgcggg aagattcggg agtggccagg aacagctatc acatgagggg
     1381 catggctgcc gatgtctgca tggatggcgt ttcgctgaag gatatgaaac ggaccgcaaa
     1441 gactctgaag gggggaggcg tcggtgccta tagggacttc gtgcacgtgg acgtaggccc
     1501 ggtgcggtcc tggtagaatg gctcaagggg gcgcccgctt cttgagtatt acggtcccgt
     1561 tgtggaggcc cctgcatccc gaaaacggat cagtctttgg agacttcaat acggggaggc
     1621 gtcccagctc gcatgctgta attcaccctt gcgaatggcg taatccttgc ttaatcccct
     1681 gatcgtacat tcgttcggca ctcttccttg tgcactcgga gagtgccgtc atccagcgtg
     1741 gattcttgaa aaagaggagg agagactggt gagaggaaag accgcgtttt tccctgtgct
     1801 cctgtggctc atgatttggg cggctatagc cagcgctgcg agctcgaagg caattgacga
     1861 atcgaacgaa tacggaggaa ttaccgaaga gcggactttt tccgcgggtg acgccgcttt
     1921 tgatgtagac ggcatacaga agcttgtgtc gtattacgac ggcaatggga atgtccgcgt
     1981 gagggaaacc catttcacgg aagcatcctc tggcaaggat ggtgtcgaga agctcgtacg
     2041 gcggttcagt gataggggaa gcccggcgag tgacgaatac gatttcaggg atgattcgcg
     2101 cattgccaag ggcgtcgcca ggttggtcag gcattacggc gaagacggaa gaaagaccca
     2161 tgaagagtac ttctacacgg acacctcatc gaggaaggat aacgtcttca ggatggttcg
     2221 gtatttcgat gcaaaaggaa agaaaattcg cgatgagctg cactcgacgg gcacgtatgc
     2281 agccatgacc ggcttttcga agctggttcg cgcctatgac ggcgacgaga accctgccgg
     2341 tgtccaggtg tatgaccgcg acggcaagat cgtggaagaa gagccgaagt cgcgctgaca
     2401 gcgaatcttc aagccgggtg gttgttttgc cgcccacaag ggctgtttcg agaatagcat
     2461 cggccctctt cgaggctatt cctgcttgaa agatctcgca cccatcgtcc attgcggagt
     2521 gaacaaatcg tgcaacagcg ttggaaaaaa gtttcatttg atgaggactg gttgaaaaga
     2581 agtttcaatt gatgaggact gattgacctt gttttggcag aaggttagta tttaagcgtt
     2641 gccgtcaaca tcaaggtgaa gcttttatga acaggaaacc tatcgtatgc ctcctgctgc
     2701 tcgtggcttt cggcgtctgc ctgcttcacg cggctccgat gtcgcaggga gaggctgatt
     2761 acgggtatcc ggggcatttc cactgttcga gtcctggaca cgtgttcgtc ccgtccgcag
     2821 cgggattagc cgatctttcg gtcttgccgc tggcgggccg gcctttttcc aaccgggtca
     2881 atccagccct ggatgatctt gtgctcttgc ttttcaaacc tcctcgattt tccgcctgat
     2941 tttttctcaa agccttctta gatccatatt aagaggaaga agaagcacgc tccaggcagg
     3001 tgacttccac ttaaagcacc gcctgccgcg agatatcttt ctacaactaa ggaaaaaaag
     3061 catgggaaga ctctgctcga atccattgag cgccgtgctg gctccggcgt ttctggtact
     3121 ggcgttgacc ggttgcaaga gcggcggcag ggtacgcatg cggttcctcg aagaaacacg
     3181 aaagtgacca gacgcggtga tcggatgatc aaggagtggt tcatatggat aacgtttcac
     3241 caacctatac gtgggagtgg gaaggagtca tttcgggcct cgactgcgcg gattgcgccg
     3301 ctaccctcgc acgggacatc gagaaaatgg aaggcgtgaa aacagcctcg ctcaattttg
     3361 ccacctcgag gctcaggatt gaatacgatc cggcctcctt cacgcttaaa aggatgcaaa
     3421 aggagctgag aagggccggc tacggcctgg cgcggcaaga tgaattccag cgcgccgtcg
     3481 tcatgatcgg cgggatggac tgcgccgatg aatcgaccat catcgaaaag cagctcaaaa
     3541 gactggcggg cgtgggagac ctcaagttca acctggtggc ccgcgaggtt gtcatcgaat
     3601 acgatccttc cagggtcaag ctcgaagata tcattcgagc catcgatgcg accggcatga
     3661 agggaaggct cggggggagc gagctcgccg cggaatcttt ctgggagcgg cacaagcacg
     3721 tggtcctcac ctgcctctcc ggagccttca tactggcggc gtttctcttc tcatgggccg
     3781 gctaccccca cgcggtgaca gaccctctct atgttctggc gatggttaca gggggctggt
     3841 tcattgccag aaaagggctg atggccgtta gaacgttgag cctcgacatg aactttctca
     3901 tgatgaccgc cgcaatcggg gcgggagtca tcgaggaatg gctggaagga gcctcgatcc
     3961 tgtttctctt ctcggtggcc aacatcctcc agaatctgac catgaaccgg gccaggaaag
     4021 ccatacgctc gctcatggag ctctctccca gcgaagtcat cgtaaggagg aacggccggg
     4081 atgaaagggt gccggtggac gcggttcgga tccgggaaac gatagtcatc aggccgggag
     4141 aaaagatcgg cctggacggc cgtgtcgtgg aaggctcttc ctatgtcaac caggccccca
     4201 tcaccgggga atccatggcg gttgagaaaa acccgggcga cgccgtatat gcggggacca
     4261 tcaatcagaa tggctcgctc gaagtggaag tgacccacga ccataaggac accactcttt
     4321 cgcggatcat ccacatggtc gaggaagccc aggcacagaa ggcgccgtcg cagggcttcg
     4381 tggaggaatt cgcaaaatac tacacccccg ccgtcatagg tgccgccgtg ctggtggcga
     4441 cggtgccgcc gctggccttc ggccttccct tcgatgcgtg gttctaccgg gcgttggtgc
     4501 tgctggtggt ctcatgccca tgtgccctgg tgatctcgac tcccgtttcc atcgtttcag
     4561 gccttaccgt tgccgcccga aacggggtgc tcataaaagg aggcgtatac ctcgagaaag
     4621 caggctcgct taaaacgatc gccttcgaca agacgggaac tcttacaggc ggcgtccccg
     4681 tggtgacgga cgtcgtggcg cttaacggcc attcggaagc ggatgtcctc accctggcgg
     4741 ctgccgtgga atgccgctcc gagcaccaga tcggggccgc catcgtgcgg gaagcggcaa
     4801 aaagaggaat aagctgcgcc gtcccggccg attttcaggc catgaccgga aaaggggtga
     4861 aagcccggct caacggtcgg accctctata tcggcaatca caggctcatc aaggagctgg
     4921 gcaaatgcag cgctgtaatc gacgcaaaga tgctcggttt cgaaagtgag cggaaaaccg
     4981 ccgtgatcgt ggccgacgag gaccaagccg tcgggatcat cgccatctcc gatgaggttc
     5041 gcgcagaaag ccgggctgct atcgaggagc tcaaggcgag cgggatcgaa aagattgtga
     5101 tgctcacggg ggataatcac gggaccgcgc gagccattgc cgaatctctc gacgtggatg
     5161 aggtccacgc cgagatgatg ccggaagaca aggcaacgat cataagtaag cttatgaggg
     5221 agcacggcaa ggtcgccatg gtcggcgacg gtgtcaacga cgccccaccc atggcccttg
     5281 ccaccatcgg tatcgccatg ggcgccatcg gcacggatac cgccctcgag accgccgaca
     5341 tcgccctcat gaccgacgat ctttcccggc tgccgtttct ggtacgcctc agcaggaaga
     5401 cggtcgggac catcaagcag aacgtggtgt tctcgatcct ggtcaaagcc gtctttatta
     5461 tacttgccgt ttcgggcctg gccaccctgt ggatggccgt cggggcggac atgggcgctt
     5521 ccctgctggt gatattcaac ggcctgaggc ttcttgccgt aaagggaaag gagtgatcgc
     5581 gcgagtgttt gaactgatgg ttcaagagtc ggcaatcccg actcctttcg ttaatgatga
     5641 gaaatattgg taagaccggt aacatcgtca gtatcgatga tagaggagat agaagaatga
     5701 tcggcaaatg gagaaaatcg ctcgaaaaca ggcgctatgg attgaagacg gtgacggccg
     5761 tggccatgtc atttctgaca gccggggttc tcctgacggc cgggttcaac tggacgggtc
     5821 cctcgtccgc gcagaacagc gaagcggaga agcacgtgag cgcggaaccc tcggcacccg
     5881 ttcctgcccc ctccctcgct ccactgttcg gccaaccttt tggggcatca ttcgccccgc
     5941 tgacggagaa gctcacgctc tcggtggtca atgtgaaggt gaccaagatc gaaaacgcgg
     6001 cgatacatgg gcctcaaggc cctggtgagg agccgtacga tccctatgat gagttcttcg
     6061 gcaacggcga ccggtttcac cgcaacgaag gccggtttcc gggcaacggt ggaaaccagt
     6121 tggtgcaggg ggccgggtcg ggggttatca taagcagtga cggctccata ctcaccaata
     6181 accatgtggt ggaaggcgcc agggaggtga gagtcatcct gggagacaag cgtgagctca
     6241 gtgccagggt cgtagggcga gaccccaaga cggacctggc ggtattgaaa gtcgccgccg
     6301 gcaaggacct gccggcggcg gcaatgggcg actccgaaag tttgaaggtt ggcgactggg
     6361 tactcgccat cggtaacccg ttcgggctgg gcgagaccgt cacttcgggg atcgtgagcg
     6421 ccaagggccg ggtcatcggg gccggtccgt atgacgactt cattcaaacg gacgcctcga
     6481 tcaatccggg caattcaggg gggcctcttt tcaacatgaa aggagaggtg gtcgggatca
     6541 atacggccgt catggcccac gggcagggaa tcgggttcgc gatacccatc aatacggcca
     6601 agccgttgat tcctcagctc gtgagcaagg gaaaggtgac ccgaggctac ctcggggtca
     6661 atattcagag catcactccg gaaatcgcct cggccctgaa gctcggtgag cgcaagggag
     6721 cccttgtggc cgacgtcttt gacgggtctc gagcggaaag ggcaggcatt gaacacggtg
     6781 acctcatcgt tgcctacaac ggcaaggaag tgaaaagcag ccaggatctg gccacaatgg
     6841 tcgcatcgag ctccgtgggc gaggaggtca ccgtgacggt cctgcggcac gataatgaaa
     6901 aggagctcag gattagaatc gcaagactcc agccggaccg ggccgacctc gaagagctcg
     6961 aaggacaatt ccaggaacaa ctgggcatga tgctggagga cgtgacacct cgagtggccc
     7021 gggagcatcg aattaggctc gaatacggcg cgtacgtcgc ggacgttcag ccgggaggcc
     7081 ctgccgatga ggcgggcatc caggaagggg atgtcatagt cgaggtgaac caccagccgg
     7141 tgaaatccgt cgatgaggtc gaagagcagg ttgccggggc ggacgaccag gctccggtcc
     7201 ttttcctggt gcacaggggc aggggaatga tctacatcgc cctgtcaaac agggcacaaa
     7261 acccgtgatc cagctccatt cggggtgccc gggagtgcga ctcctggttg tggaagacga
     7321 gaagaaagta gcctgcttca tcaagaaatg cctggaagag gaaggcttca gcgtcgatgt
     7381 ggccctggac ggcgaaaccg gcattcgttt ggccacggcg gaagctcatg acgtgatcat
     7441 cctcgatttc cgcctgctgc aaatgagtgg aatcgaggtc gtgggagcgc tccgccgcaa
     7501 tggctcggcg gcgtccgtac tgcttctatc cgccaatccc accgtcgagg aaatagtcct
     7561 cggactggac tcgggcgctg atgacttcct cgccaaacct ttcgtgtcca gggaattggc
     7621 tgccaggatt caaacccttg tgagacggcg cgcccaggtg cagcgggcgc accttcgcgc
     7681 ctcccccggt tgccatcaag agagtgagaa atgagtgaaa cgtcctgaaa ttcagggcct
     7741 aaggcccgtt ttcccctgag accagatccg tgtactccac ctggagtgtc gtgtgatcaa
     7801 taccgaattc attcttaagc cgttggttga gattttcgag gattcgttgt gccgcatcgg
     7861 ctttggttcc ttcaacaagc acaacatgtc cgctcaaggc ttcgatcccc gatgtgattg
     7921 tccacacgtg gaggtgatgc acggtgagaa ccccgggctc ggcgcacatg gcatcctcga
     7981 ccgccttcgg gttgatatgc gagggtgtgc cctcgagtag gatgtgaacc gcctggctca
     8041 tgagcttcca tgtgcgaggc aggatgaaga gaccgatggc cacaccgaaa atcggatcgg
     8101 catagtacca tcccgtagcc gccatgatgc ctgcggcaac gatgacaccg acagatccaa
     8161 gggtgtcact cacgacttcc agaaaggcac cctgtacgtt cagactctcc ttcgagcccc
     8221 cacgaaggtt ccagatgccg atcaggttga cggcaaggcc taccacagct acggcaagca
     8281 tgggaaggct tgccacctcg ggcggggatt ggaagcggcg gtaggcttca taaacgatgt
     8341 agaaagagag cagaaaaagg atgacgccgt tgaggagcgc cgcaaggatc tccacacggt
     8401 aatagccgta ggtcttgctc ggggttgccg gctttgaggc tatccaggaa gcaaacaggg
     8461 ccagggccag cccgccgaca tccgtcatca tgtgggcggc gtcggcaagg agcgccagac
     8521 tgttcgtaag aatcccgcct atgagctcaa ccaggaagta tatgaatgtg aatgcaaagg
     8581 taacccatag tgagcgtctg tgttgcccca tcgcggaaag ttgcttcgcc atatcgtgcc
     8641 tccttgtcca acatcctcct gctcaaatca attccaaaca agggtggaag gaagttgtcg
     8701 tcggtctgtg ctttaacagt gctcgtgccg gtggtgaata tccggatagt gcggatggtg
     8761 atgcagcact tcacagtgcg cgtgcacgtg ggagtgggcc gtgtcatcct gcagcagaca
     8821 gtcggcgtga tgccggtggt gctcatcatg aacatggctg tgattgtgct cgaaggcatc
     8881 gtgctggtgc tcgtgctcat ggcactcggt aagatgcagc cacacaccga tgcccatgag
     8941 gacggcgccg gcaaggaaac tcagggtgac accttctccg agaatcagaa tcgcaataaa
     9001 agcgccgacg aagggagcca aagagaagta cgctcctgtt ctggcagttc cgaggtgtct
     9061 cagggaaaga acaaagaaca tgagactgat accgtatcca agaaacccca cacaggcaga
     9121 ggcaaacagt ggccacggag cgggaagagc ggcaccgctc gccagggcga tagccgtatt
     9181 gacagctccc gcaatgagac ccttggccgc ggcaatctca agcggttctc cttccgaaac
     9241 cttccgcgtc agattgttat cgattcccca ggaaaagcag gccccgatca cggcaagaga
     9301 cccccaggag atgaagaagg gctcctcacc cgaccacgat agaacaacac accccaaagt
     9361 gataactacc atgcccagta ggatgcgccg atcgaagttt tccttgaata caaaccaggc
     9421 gagcgttgcc gtgaagaccc cttcgagatt caggagaaga gaggttgaag atgccgacgt
     9481 tgccttgagc ccgatcatga gcaggacggg agctgcaaca ccgcctgcca gaatggctcc
     9541 tccaagccat gggagatcgg ccgctcttag agaggcctct ccattgcccc tcttctgaaa
     9601 ccccattcgt accagccgcc aaagcgtaag gcccaatccg gagcccaggt acaggagccc
     9661 cgccaggagc atcggatcga ctctgccgag gaggattttt gaaaacgggg tgctcgcccc
     9721 aaagagtacg gcggtcgcaa tggcaaccgg agctcctgat cgcatgaatt ttggcatact
     9781 ctttgtgacc cttcggactt gcatgttgcc ccctgaactt tggaccggga acacagggaa
     9841 cccgtctctt ctataaagtt aaagcctcag aggcatattc gcccttactc actacagagg
     9901 tgcgcatcct tcttacacac cttttgcccg gtaagctagt cctgcctgcc ccggtaatgg
     9961 gactggtcga tccttttcta tccagaggcc acatgcgtga tcacggcaac agatgcatac
    10021 cgaacgagct ttccaccaaa cacgagcaaa gagaatcgga ggaagcccac tccgaacagc
    10081 cagccggcaa ggcagagggc gtcccccacg atgggcagcc atgagaagaa aagggaccag
    10141 acgccatagc ggttgtagta cctgcttgcc ctctcctggg aggcctcatc gatgcgcagc
    10201 acttttcgaa tcaaaatagg ggacccgtag ctgccgatcc aaaatgtcga gcacgccccg
    10261 aggtagttgc caaaagtagc gaccgccact gtcatggctg gactgaagcc tttgaggatc
    10321 agagccacga gcagccattc cgaacctacg ggaagaagtg tcgaagccgc aaagctcaga
    10381 aaaaacaggg caggatagcc gccacagaga agcatctcgt tcatcgggat gcccttagtt
    10441 ggaaaaagcg gcagcacctc tgccggagga gcccgtagat cacgatctgt taagcattgc
    10501 gggctaagat tgggtgcccc tgattataac caaggtcaaa tgctttctcc agttcttccg
    10561 ccccgccaag gtaagggggg ccgagtaatg cggccggttt gccaaaaaga ccttcaccaa
    10621 ggcaagcccg cagttcgtcg aagaacagtc catgctcgtc gggggcgacg ccaagcacat
    10681 agttcgccag tgcgtcatgg acctcatgac ctcaccattc cgcctacatc gaaatctcgg
    10741 atcatggccc ttccaccatg cctgccgcat gctaatcggg aggtcatcct ctgctacatt
    10801 ccgaggaagt cagctggctg ggctcaactc aattctggtg tgatcacagg ttaatccatt
    10861 cccgaaaagt gctctcttcc gggatcgacc tgcgaggtgg aattgtcaga gtagggttcc
    10921 ctggcgatcg cttgatagcc gccggtatag cgtggaagag cctccaggca agacggcacc
    10981 gtgaaagcac agtgcgaagt caggacattg gtttagaaca cgatcacggt tcagttgctc
    11041 tcatctctac tacggtaaac gatcgtggca gcaatgatga cgggtccttg cgctcatcag
    11101 agtccagcca gaaggcatgc tcgcgggcga cactggcgga tgattcgatc aagtcccgga
    11161 gctgcattcc atccatgggt ttggggccat tttccgtgtt cttgctgacc cagtcggtga
    11221 acgagacgtg tcgcatgtaa acgggttgac tacaccagaa gccccaattg tcagtgggat
    11281 tctgaatagc tatgaaatgt ctttctcctt catcaaaggg cggcgttcgg tccgtgactg
    11341 gtacgcacct gaggtgattc aaatcaggcg gatcatagat ggcatcgata ttgaagaaca
    11401 tcgggcaggc atatgcgacg gatgctccgt ttatcccgga taaagctatg agcaggtggt
    11461 gctgtatggc accggattcc ttcgatctcg tcaggtccag ctccgagcgg aagtagtctg
    11521 cagtgaagcc ccgaggagag tatctgcttc tccggttcat tatctccact actttgaact
    11581 gaagaaaaaa gccgaagtag aaccgggaaa ggctcgttgc tccggatggg agcgcggttt
    11641 ccagctcgga atagaactgc tcattggaga gctcggttaa aacccaaccc tgatcgaagc
    11701 ccacccagcc ctcttgcctc tggctgggct taagaatgcg gaatacacga gccccaaagg
    11761 ccgaagcaaa atggtctctg atggcctgaa agtagtacat ctccagtaga gcctccgtga
    11821 cgatcttgcc cttcataggc tttccccctc tcaaacgagc cggacaacct ctgccatccg
    11881 aagacctccc aaaattgact cacggggctc cccaaatttc cagtgattcc tcgctgtgca
    11941 acaaagttct cagctttgta gcccgctaat ctaacgtgcc ctgtccttgg gaactgtcga
    12001 cgtgttttca tcttcccaaa tgctgacgtc catttcggca gcataggcca gagccgacag
    12061 aatccgtttt cccatcttga gaacttcctc ctgccttgct cccagtacgg gatgcactgc
    12121 atcctttggt gtctttccca gcacacagcc cagaaggtgg taatccagtg catagggaac
    12181 ctgtacagca atgtccgggc gcgatatcaa ggccttgatc acttcgatga tttcgtccgt
    12241 ataatgcggc ggaggcagac cttcaaaaaa gtcagtccca aaagcgagct ttgcaagttg
    12301 ctgtgccttt accggagacg tgtccccgta ggtgagcttc tttcgggcgt aagctgacca
    12361 gtctttttca ggtgtccact tcgattctgc ggagaacctg acgagcatga cagcagccac
    12421 tacggcggcg ttgaagcaaa tgtagcggct tatccaggca acatcgtccc tgataccggc
    12481 gggctgtttc ttaatcatcg agaacgttgc caccacgcgg ttgaaattcg taagaggtga
    12541 gccaatcgtc aacatctgcc tcagtttgag gtcctccctc aaaaggtgtt tattctcttc
    12601 ccccatatgc ttcttgcaga cttcgaattg attggcgact tcaggatccg cttcaccaaa
    12661 ggcctggatc tttccgcccg cactatcgag aaatgtctcc aggtccagcg catccaggat
    12721 ttcaaccgcg ccctgagccg cgaactcgcg ggcttgccag gggactttcg gagtcacaac
    12781 caccgcccgt tgcgcacgcg aaaaggaaga aaggccgagc gtccagagta tgcgctcaaa
    12841 ggggtgggac ttctttcggt ccttgcagtc cacaacaacc cgttcctcgg cgagaggagt
    12901 gttgaaccga atcgcaagga gatcgatatc ggttgcctcc gcagtgcctg ccgcgactga
    12961 aagctttacg cttcgtctga ccaggtatcc atgagcctga aagtaggagg cggttagaaa
    13021 ctccaggctg tttcccttct tcttttcgat cggcttcatg tcctcaacgc ctcaaggaat
    13081 cgtttcatgg tttcaggatc gttctttacc agcctggatc ttgcgggcac tctggatgcc
    13141 tcgggaccta cgaatcctgt cggcagctcc agactttgct gcagcatgct cccactcacg
    13201 gtcgatacgg aacctgactc gagaatattc aacacgattt cagcaggctc cctccggcgc
    13261 agctccatgt aaaactggtt tcccggtttg tgacttgccc ggactgtacg gataatcccc
    13321 tctccctctg gcagggtgta gtcctgacct attgctgtac aaggtcccac tcggtcgcgc
    13381 tgaagcaagg cattgacgag cactggcgga tcgataatcc tgccgcgtga cagcgaggag
    13441 cgtagctcac caaatcgcag agacgataga agtatcttcg catggtggaa gacatcctct
    13501 tcaaggcgct cagtctcgac gcctacagac gggggagcga gtcggggcag agtaaggaat
    13561 tttgcatcgc ctgccgagct tggcactttc gatacctcga ccaatccaag tccgatggcc
    13621 atcttcctga tctcaaccgg tatccctgta tcttcaggtg gaatcccagg gcggctggcg
    13681 actttctcca gtagctcgtt gagtttctcg cgtagttccg tggacagggc tgaaagagca
    13741 ttcccaatgt cctcggcgcg attgccgaac tggtaactgt tgaaaaagag ccgatcgctc
    13801 tttgaccgaa taggcaaaac cccaaggagc tcgaaatttg ccaccaggcc aaggaggtga
    13861 ttcacatcct tctccttcaa tctttcagag agaaaggctt tcgcttctgc ctccaggcgt
    13921 ggtcgaacca aacagtattc aaggaattcc ggcaaaacct gctctatggg tgtaatccct
    13981 acagcgccaa ggaggatctg cgaagccgtc tcaaggacgc gctgtcgtgt aaaggccaga
    14041 acctcgtaaa cagagtgcgt gttgtcccaa tcgatgcacc cggaggcctt tagcgtatcc
    14101 aggtagatcc ttagctccgg gccgctgatt ccgcatagtc gtgcatgtgc cttcactttc
    14161 gtatcggaga gctgttccgt tgcatcggct gagatcttga cgagaagact gccgcacttt
    14221 ccggcgaaaa agaggttctc cagtgaggtg aagcttgggt tcgtattgtc aaaattatac
    14281 aggtgcttgc tggtgttgat gatccatgcg cccattctgg attttgtcat tgccggcttc
    14341 gtgcccgcta tactcattca ttactcctgg ggaactgtct tgatggcgtt cctgtctttg
    14401 ctcaatcagg actacgtctt tgcctctgtc accgatgcat ttgcgcacac cgatcgagga
    14461 acacttccag cgatgccggc cgttttgccg tcaggccggt ctccatgatg agttaggctc
    14521 cattatgctc ccgtttttgt ttacaatcaa ggtagagacc ggtccatttt ccagacgtga
    14581 ccggtcgcta tcgctatcat tcctttcttc gtgcaagcgg gcgcattccg agatcacgtg
    14641 tcaattttca cttgaccatg ggaacagtta tgtctaaact gggttaaaaa tgcgccgtat
    14701 tgtaagcttt ttcaactgaa ggcggggttt aggaagtgta tccgacacgg ctggtagaga
    14761 gactcaccgg ggcgactgcg aatcagttga agtattgggc gcggaccggc ttggtttgcc
    14821 ccgcgagaga agggaaatgc ttcttctttt ccttcaagga gatcgtcaag ctgcgcgtgc
    14881 tggtctcgtt gagaaagaag gggctgagcc ttcaaaaggt gagagtcggg atagagaatc
    14941 tggcctccat attgccggac gctgaacccc tcacgcgcct gatcatttac acggacgggg
    15001 tggacatgat cgttgcggag aaggggaact acttcagtgc aataaccag
//
LOCUS       DAOOVC010000097        14873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2692_length_14873_cov_5.676407, whole genome shotgun sequence.
ACCESSION   DAOOVC010000097 DAOOVC010000000
VERSION     DAOOVC010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2692_length_14873_cov_5.676407"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLM79_12175"
     CDS             <1..1192
                     /locus_tag="PLM79_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87113.1"
                     /translation="DFFYQLRRAGIPVSLRYVLELYRGLQRGLAANLDQLFLLSRLIF
                     VKKLEHYDAFEQVFSSFFLGRETEGKPMDWEDLLAGKPFHDWLRDQIGQGLLTPQQLR
                     DYDTEELLRRFWETVLAQRGEHHGGNTWVGTRGRSPYGHGGLHGGGVRVHGSGLYGTA
                     QKVIEKRNYLQYSGASRLSTENLRQVLASLKSLQPAGPETDLDVDESIALTARNGGEI
                     ELMFRRELRNRLKVMVFLDNGGWSMAPYVNLVRTVFRRVRDVCRELKVFYFHNCIYGT
                     VYEDEPRTRGVPWEKLLNESRDSRVILFGDANMAPAELMASYGSLDVHSMVRKPGIQW
                     LKELRNTFPCSVWLNPIPKDRWSRESSTIRTLGRIFPMEDLTLGGIQAAVAVLNTQGR
                     ALDR"
     gene            1230..2012
                     /locus_tag="PLM79_12180"
     CDS             1230..2012
                     /locus_tag="PLM79_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87114.1"
                     /translation="MSYLDQEVRRLLGRAVQGYGLIRDGDRIAVAVSGGKDSLLLLWL
                     LRERLARIPIRYDLVAVHVDPGFDQETSERLEAFFRSEGFDYEILHTDHGIRAHGPEN
                     RENPCFLCARLRRTALFQAASRLGCGKIALGHNQDDFIETFFINICYSGQVSTMVPRQ
                     SFFGGEIVVIRPLALVPAARLRRLAGQLGLPVLENPCPSAKANKRHEVRSLLRDLYRK
                     NSKVRGNVFHAMSHINTEYLPPPLRPQHRKNPPPPETTEDEP"
     gene            2009..4708
                     /gene="polA"
                     /locus_tag="PLM79_12185"
     CDS             2009..4708
                     /gene="polA"
                     /locus_tag="PLM79_12185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOV87115.1"
                     /translation="MNSKKTFYLIDGSSYIYRAFYALPRLTGPRGMPTQAVNGFARIL
                     RKVLREKEPDYVCVVFDAPGPNFRHSLYEAYKATRQKMPEDMAVQIPYIKDLVRCWGI
                     PQLEVAGYEADDLIAALTRTAREKGVDVTIVSGDKDLLQLVRDPSVRQWDPQKDKVYE
                     EKTVEERFGVQPAHMRDYLSLVGDSTDNVPGVKGVGDKTARQLLERWKTLDNLYAHLE
                     EVSRESLRKTLGEHKEEAYLSWELVALKSDAPVEEDLSRYTPSPVLGPRLRRLYEELG
                     LRKLLEELPQEEADEAPASTEAVEKPPWEDRRVTSIEELHRLVERLRGEESFSFSLDV
                     APSNPMRADLRGIAFCREDRVSYYVPLAEKDAGDGSGIPLQAVLDEIRELFGRSTPVK
                     IGADLKSSWIILRRYGVELSGMDLDPTVANYLLDPGAQGHSLDRLVQEHLGETISGGN
                     ESRNTEGPAPPSSSRNAPAASRDAVLCRRLAPVLRRKLEETGLYPLYRTLEFPLIRVL
                     ADMEYRGIRVDSRRLEDLSLELEKAMDQRAGVIFQLAGCSFNIQSPKQLSHILFDKLN
                     LRVLKKTKTGPSTDVRVLEELAGDHPIVEQVLAFRSLAKLRGTYAESLPRLVHPETGR
                     IHTSYNQTVAATGRLSSSDPNLQNIPIRSEEGRKIREAFVPSTGSLLLSADYSQIELR
                     ILAHYSRDEHLTEAFRTGEDVHQRTAMELFGVAADQVLPEMRRRAKTVNFGIIYGMGP
                     FGLAQRLRIPNPEAKKIIEQYFRRYEGVRKFIDDCVEQARLRGYSETLLGRRRPIPEL
                     QSRNQNMRQLGARLAINTPVQGTAADLIKKAMIDLFQALEGRKMKTAMLLQVHDELVF
                     EVPRGELEEAKELIREGMEKVWELAVPLRVDMGWGTNWAEAHP"
     gene            complement(4781..5065)
                     /locus_tag="PLM79_12190"
     CDS             complement(4781..5065)
                     /locus_tag="PLM79_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87116.1"
                     /translation="MKVFVGGLVAAFLGLIGIFAFFGKFMQLMAGAVPLMLLVGGAMA
                     AYLGYDEVKDKLPFPKKKEAEGAQAKDEAERYKAEVERLRAELEKAKAGS"
     gene            complement(5278..5805)
                     /locus_tag="PLM79_12195"
     CDS             complement(5278..5805)
                     /locus_tag="PLM79_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87117.1"
                     /translation="MTVRARRALFPAFLGLVAAVGSFAAEPRIAVESDLVAQAPEESS
                     TATEITEESAPVRNLGGILDEIRVPDRTAREFTQLYLNHRQELLRILSDRPALIWQTL
                     ELVLEALPALKAVEEREGRIYLDKKLYRKVDSFLGTFEQYCSLELASDLRKIQRFVLD
                     RMEEIPNDQVVIDLN"
     gene            5915..6973
                     /locus_tag="PLM79_12200"
     CDS             5915..6973
                     /locus_tag="PLM79_12200"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOV87118.1"
                     /translation="MRLVALLKRKRFWVAASVGFFILVYTIKKADLETLLENLQDIGR
                     GWALVAVAAGLVSYLCIAAVLHYLLRGTNHSLPFSTTFRISLLSCTLNYIWAMGGLSG
                     VAAKVYLLAKENISPSSTLSISMVHGFLTNTVAVLFIYLGFFFFYSKNKMNAEQMELA
                     VVLLLFAFVLTWLTIQTMIHESFRKRLWQFLLYIANAVCRKLRRPHWIKQDRAESFFR
                     HFNANMNLLVTNRRILLAPASYALLDWFFMFLCLKYAFYAVHYPVDNWTLLVGFSVGI
                     FANLFSLTPASIGIMEGSMAASFYWMGLDYNHALLATLLYRFAYFFLPILISIFYYKQ
                     FFRSSVEEIEVEEVPKKT"
     gene            complement(6983..7435)
                     /locus_tag="PLM79_12205"
     CDS             complement(6983..7435)
                     /locus_tag="PLM79_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87119.1"
                     /translation="MTSLFPNDRQREHGGPAAWDRRVTWLLVLLLAVPLPFARFETWP
                     GGAVISEHPVTPWSRFRICFWEPPEGDPREDRYEFTWRGQVLPRESAPPLFLYVPSLR
                     PPALKWQDNPEIPLNHSFFQGQLIRLETYWQPLVLWPLRMLWRSVSGG"
     gene            complement(7496..8287)
                     /locus_tag="PLM79_12210"
     CDS             complement(7496..8287)
                     /locus_tag="PLM79_12210"
                     /inference="COORDINATES: protein motif:HMM:NF017944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HOV87120.1"
                     /translation="MKTGILLTAFGTRVPEARAVLGSVASPVREHFPDRVVRWAYTSG
                     VVRRKLAEEGTFIDSPEAALARFAEEGFERIDLWSLHVIPGKEFHDLARTARGFHRAK
                     HGLPEIRLTRPLLSSYEDLRRVVQCLLEGLPAERNPEEEAVLFMGHGSAHHPADLIYA
                     AMNFALGEEDENVFVATTQGFPSLAQILPRLDRGRLRKVHLLPFMLTPGEHIRTDMAG
                     DHPESWKSILEHRGLECSISLKGLAENPGVMEIWLDHLRNAQPLG"
     gene            8379..8678
                     /locus_tag="PLM79_12215"
     CDS             8379..8678
                     /locus_tag="PLM79_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87121.1"
                     /translation="MTVDEVRKRLDEIEQLAGDLLETPEDERIGIYRAMDPLFLQLEG
                     WWRESPDPSLLEQVEELKLRILVTARLQEPDGESDESRHLWALEILESLRSAVAR"
     gene            complement(8826..9017)
                     /locus_tag="PLM79_12220"
     CDS             complement(8826..9017)
                     /locus_tag="PLM79_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87122.1"
                     /translation="MSAILKKDGKPMGLFPLKFEGTPSHFAGDLKIEETGEYEAIVYA
                     YDPANGNTGLDSVTFKAVK"
     gene            complement(9066..9602)
                     /locus_tag="PLM79_12225"
     CDS             complement(9066..9602)
                     /locus_tag="PLM79_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87123.1"
                     /translation="MGKVCRLLGLGLLLGMLSASAAWAVPTEIIVRVKTKDAKFLGTN
                     MGGALVTIKDAHTGELLAKGRTAGSTGSNERIMETPHRRGVPLSDETSAKFLAVIDLE
                     EPRLLQVEAMGPLSGLQAANRASNTQWVLPGKHITAGDGWMIELPGFAVKVLSPSPNA
                     SVQSLPNTVPIRAYAALM"
     gene            9866..11398
                     /locus_tag="PLM79_12230"
     CDS             9866..11398
                     /locus_tag="PLM79_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HOV87124.1"
                     /translation="MSNVRSFTRLTPQEAALHISHGNTVAFSGFANAGAPKAIPRALA
                     ARARELHDRGEPFKIRVLTGGSGGADIDEALSRAEAVSWRAAFQSSPTLRKQINRQEV
                     EYVDMHLSHVPTTVSAGFFGKVDVAVVEAVEITPDGRVYLSTSIGASPTFLRCAERVL
                     IEINASHSPRLREMSDVIILSPPPHRNPIPIQGPLTKLGWSYAVVDPRRVMGVVENNE
                     PDDVPPFDQPGAASEQIAEHVVRFLFDEMRVGRIPESFLPLQAGVGNIANGVMAALGR
                     NPYVPPFKMFSLVLQDSEIGLMEEGKITGASATSLTLSPEVLDRVYRHMDFFVPKIVL
                     RPQEIANHPAVVRRLGVIAMNAVLEVDIYGNANSSHVYGTDVMNGVGGSGEFIRNSYI
                     SIIMCPSVAKGGRISSIVPMCPHIDSNEHSIQIVVTDQGLADLRGLGPMQRAKAIIEN
                     CAHPAYRETLYRYIEESRLGHIRHNLRTCFELHRNLARYGAMLPDLELWDEGEQPKPA
                     SR"
     gene            11461..12963
                     /locus_tag="PLM79_12235"
     CDS             11461..12963
                     /locus_tag="PLM79_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HOV87125.1"
                     /translation="MPFPILSPEEAAAHVFDGATVAFSGFTNAGAAKVIPRAIAQYAR
                     NLHDGGKPFKIRVLTGASSGASIDEPLARADAMSYRAPYQSGPRLRTQINRQEVEYVD
                     MHLSHLPQTVLSGFHGKLDFAVIEANEITPDGRVYLTSSIGASPTYLKYADRVFIELN
                     DYHSMRLREMADIFILPPPPHRSPVPLHEPLTRVGLPYAVVDPRRVLGVVKTSEPDQV
                     PDFAPSDEKGRRIAEHVVRFLVDEMRAGRIPDEFLPLQAGVGNVANGVMAGLGDSPDI
                     PRFKMYTEVFQDSLVDLMERGKLLAASTTSLTLTPAVLRKIYDNMDFFVPRIVLRPQE
                     LSNHPGVITRLGVIAMNTALEVDIYGNVNSSHVFGTDIMNGVGGSGEFTRNAYLSIYM
                     CPSVARGGKISAIVPMCPHVDNNEHSVQVVVTEQGLADLRGLGPMQRAQTIIERCAHP
                     LYRDYLHRYVERSRVGHIRHDLELCFELHRNLMETGSMLPDRDLSAADVS"
     gene            13249..>14873
                     /locus_tag="PLM79_12240"
     CDS             13249..>14873
                     /locus_tag="PLM79_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOV87126.1"
                     /translation="MATRKILLGNEAIAHGILESHCTVATSYPGTPASEVLRTLIRLR
                     EEHGLDLHLEWSVNEKVAFEVALANSYVGRRSAVVMKQVGLNVAADPLMSAAYTGVKG
                     GFVLVVADDPGPHSSQTEQDSRFFAMMAKVPVFDPSSPAEARELICKAFELSEKYEIP
                     VMLRPTTRVCHAREDMEISAVSPEAPFPLFEKNPARWAATPKFRLELHRVLNEKIHRL
                     LEETDRPPRLVNEDSLPSQPDPHSRRKESRSCLVASGTALAHLCEVLRDLDLWDKVPL
                     YQVLMPYPLSSAFRETLLERYERILVLEETYPVMELQFQDAAKVHGRRNGVVPSQGEL
                     LPETVEKILREFLGLPAPREIQVPSIPGRRPTLCAGCPHRATFFAIKKAFPKGIYPGD
                     IGCYTLGLNLGVVDTVLCMGASVSQATGLYFAHRSGRKDAPVICATIGDSTFFHSGIP
                     ALVNAVVQDARFVLVIMDNGTTAMTGHQVNPCTGVDALAVAVGNVHMLEGRQAGLDLE
                     LIRTLCRRIEVPLVLHGGTGIRPAALREAIQLG"
BASE COUNT         2845 a         4687 c         4426 g         2915 t
ORIGIN      
        1 cgatttcttt taccagcttc gccgggcggg cattcccgtg agtctccgct acgtgctcga
       61 gctctaccgg ggactccaaa ggggcctggc cgcgaacctg gaccaacttt ttctcctctc
      121 ccgcctcatt tttgtcaaga aactcgaaca ctacgatgcc ttcgaacagg ttttcagctc
      181 ctttttcctg ggacgcgaaa ccgagggaaa acccatggac tgggaggacc tcctggcggg
      241 aaaacccttc catgactggc ttcgagacca gatcgggcag gggcttctga ccccgcagca
      301 gcttcgggat tacgacacgg aagagctcct ccgaaggttt tgggagacgg tgctggccca
      361 gaggggcgag catcatgggg gaaacacctg ggtgggcacc cgaggcaggt ctccctatgg
      421 tcacggcggg ctccacgggg gaggtgtgcg ggtccacggc tcgggtcttt acggcaccgc
      481 tcagaaggtc attgaaaaac gcaactacct gcagtacagc ggggcgtccc gtctttccac
      541 ggaaaacctt cgccaggtcc tagcctccct caagagccta cagcctgcgg ggcccgaaac
      601 ggacctggac gtggacgaga gcatcgcctt gacggcccgc aacggcgggg agatcgaact
      661 gatgttccgg cgcgagcttc gcaaccggct gaaggtcatg gtgttcctgg acaatggcgg
      721 ctggtccatg gctccttacg tgaacctggt gcgcacggtt tttcgtcgcg tccgggatgt
      781 ttgccgggag ctgaaggttt tctactttca caactgcatc tacggaaccg tgtacgagga
      841 cgagccccgc acccggggcg tcccgtggga aaagctcttg aacgaatccc gggattcgag
      901 ggtcattctg ttcggagacg ccaacatggc cccggcggag ctcatggcct cgtacggctc
      961 cctggacgtg cacagcatgg ttagaaagcc gggcatccaa tggctaaaag agctgcgaaa
     1021 cacttttccg tgcagcgtct ggctgaaccc catccccaag gatcgctggt cccgggaaag
     1081 cagcaccatc cgcaccctgg ggcgcatttt ccccatggag gacctcaccc tgggggggat
     1141 ccaggcggca gtagcggtgc tcaacaccca gggccgggcg ctggatcgtt aaggacctgg
     1201 gcacttcaaa ggatggatcg tcggacctca tgagttacct tgaccaggaa gtgcgccggt
     1261 tgctgggacg ggccgttcag ggatacgggc tcatccggga cggggaccgt atcgcagtgg
     1321 cggtatccgg gggcaaggac agcctcctgc tcctctggct gctccgggag cgcctggcta
     1381 gaatccccat tcgctacgac ctggttgcgg tgcacgtgga cccgggattc gaccaggaga
     1441 cctcggaacg cctcgaggcc tttttccgtt ccgaagggtt cgactacgag atccttcaca
     1501 ccgaccacgg aatccgggct catggcccgg aaaaccggga gaacccctgc tttctgtgcg
     1561 cgagacttcg ccgcaccgcc ctcttccagg cggcttcccg actggggtgc ggaaagatcg
     1621 ccctgggtca caaccaggat gatttcatcg aaaccttctt catcaatatc tgttacagcg
     1681 gccaggtgtc gaccatggtg ccccggcagt ctttttttgg cggcgagatc gtcgtgattc
     1741 gccccctggc cctggtgccc gccgcccggc tgcggcgtct ggccgggcaa ctggggctgc
     1801 ccgtcctgga gaacccttgc ccctcggcga aagcaaacaa gcgtcacgag gtccgaagcc
     1861 ttctccggga cctttaccgg aagaattcca aggtacgggg aaacgtgttt cacgccatga
     1921 gccacatcaa cacggagtac cttccgcccc ccctgaggcc ccaacaccga aagaatccac
     1981 ctccacccga gacaaccgag gacgagccat gaactcgaag aagacctttt acctgatcga
     2041 cgggagttct tacatctacc gtgcctttta tgccctgccc cgtctcaccg gtccccgggg
     2101 catgcccacc caggcggtaa acggttttgc ccgcatcctc cggaaggtcc ttcgtgagaa
     2161 agaacccgat tacgtgtgcg tggtcttcga cgcaccgggc cccaattttc gccattccct
     2221 ctacgaggcc tacaaggcca cccgccagaa gatgcccgag gacatggcgg tgcagattcc
     2281 ctacatcaag gacctggtgc gctgctgggg aattccccag cttgaagtcg ccgggtacga
     2341 ggccgacgat ctcatcgcgg ccctcactcg aacggcccgg gaaaaaggag tggacgtgac
     2401 cattgtgtcc ggggacaagg acctgctcca gctcgttcga gatccctcgg tccggcagtg
     2461 ggaccctcag aaggacaagg tgtacgagga gaagaccgtc gaggagcgct ttggagtcca
     2521 gcccgctcac atgcgggatt acctttctct ggtgggggac agtacggaca acgttcccgg
     2581 ggtcaaaggg gtgggcgaca aaacggctcg gcagctgctc gaacggtgga aaaccctgga
     2641 caatctctat gcccacctcg aggaggtctc ccgggagtcc ttgagaaaga ccctcggcga
     2701 gcacaaggag gaggcgtacc tttcctggga actggttgcc ctcaagagcg acgcccccgt
     2761 cgaggaggat ctttcccgtt ataccccttc acctgttctg ggaccccggc tgaggcgcct
     2821 ctatgaagaa ttgggccttc gaaagctcct ggaagaattg ccccaagagg aagcagacga
     2881 ggcccccgcc tcgaccgaag ccgtggaaaa gcccccgtgg gaagatcgaa gggtgacgag
     2941 catcgaggaa ttgcaccgat tggtggagcg ccttagaggg gaagaatcct tcagcttctc
     3001 cctggatgtc gcgccctcca acccgatgag ggccgacctt cgcgggatcg ccttctgccg
     3061 ggaagatcgc gtttcctatt atgtcccctt ggcggagaag gacgccgggg acggctcggg
     3121 aattcctctc caagccgtgc tcgatgaaat ccgggaactc tttggccgga gcacccccgt
     3181 aaaaatcggc gccgacctca aatcctcgtg gatcatcctt cgacgctacg gggtcgagct
     3241 ctcggggatg gacctggacc ccacggtggc gaattacctt ctggatcccg gtgcccaggg
     3301 ccatagcctc gatcggcttg ttcaggaaca cttgggcgag acgatttccg gaggcaatga
     3361 atcccggaat acggagggac ccgccccgcc gtcttcgagc aggaacgccc cagccgcctc
     3421 ccgggatgcg gtgctctgcc gtcgtctcgc ccccgttttg cgcagaaagc tcgaagaaac
     3481 gggtctctat cccctctacc ggaccctcga gtttcccctc attcgagtcc tggcggacat
     3541 ggaataccgg ggaattcgag tggattcccg gcggctcgaa gacctttccc tagagctgga
     3601 gaaggccatg gaccagagag cgggcgtcat cttccagctg gccgggtgtt cgttcaacat
     3661 ccagtctccc aaacagctgt cccacatcct gttcgacaag ctgaacctga gggtcctcaa
     3721 gaagaccaag accggacctt ccaccgacgt gagggtgctc gaggagctcg ccggggacca
     3781 tcccatcgtg gaacaggtcc ttgccttccg cagcctggca aagcttcgag ggacttatgc
     3841 cgagtccctt ccccgcctgg tccatcccga gaccgggcgc atccatacct cgtacaacca
     3901 aacggtggcc gccacgggga ggctgagcag ctcggacccg aaccttcaga acattcccat
     3961 ccggtccgag gaaggccgga agatccggga ggcttttgtg ccgtcgaccg ggtccctgtt
     4021 gctgtcggcc gattactcac aaatcgagct tcggatcctt gcccactaca gcagggacga
     4081 gcacctgacc gaggccttcc gtaccggcga ggacgtccac cagcgcacgg ccatggagct
     4141 gttcggggtc gccgccgacc aggtgcttcc cgagatgcgg cggcgggcaa agaccgtcaa
     4201 cttcggaatc atctacggca tgggcccttt cggactggct cagaggttgc gaatccccaa
     4261 cccggaggcc aaaaaaatca tcgagcagta tttccggcga tacgaagggg tccgcaagtt
     4321 catcgacgac tgcgtcgagc aggcccggct gcggggatac agcgagaccc tcctgggaag
     4381 aaggcgtccc attcccgagt tgcagagccg caaccagaac atgcgccaac tgggggcgcg
     4441 cctggccatc aatactcccg tgcagggaac ggcggcggac ctcatcaaga aagcgatgat
     4501 cgacctcttt caggccctcg aaggccggaa gatgaagacg gccatgctgc tccaggtcca
     4561 cgacgagctg gttttcgaag taccccgggg ggaactggag gaagccaagg agctcattcg
     4621 agagggcatg gaaaaggttt gggagctggc ggtgcccttg cgggtcgata tgggctgggg
     4681 gaccaactgg gcggaggctc acccgtagag gggtaaaaaa agccgtctcg catgagacgg
     4741 cccaggcttg cccgtaaaat gaatatctgc gaacgggagt ttagctcccg gctttggcct
     4801 tttcgagctc ggctcggagt ctttccactt cagccttgta gcgttcggcc tcatctttgg
     4861 cctgagctcc ttcggcttcc ttcttcttcg ggaatgggag cttatccttc acctcgtcgt
     4921 agcccaggta ggccgccatg gcaccgccga ccagcagcat caggggcacg gcccccgcca
     4981 tgagctgcat aaacttgccg aagaaggcaa atatcccgat gagccccagg aacgctgcaa
     5041 ccagtccacc cacaaacacc ttcataatcc ttcctccttc tcctaaattc gcggcttgaa
     5101 gagacgatat tcctttctta acggtttgat tctcctggca aatgaacgtg tggcatccac
     5161 agggccaccg gcgaaaaagt ggctaatgct tagcacgctc ccccgtagaa tgcaagtgga
     5221 aattctggtc tatacgctga tggattcccg cacaagcgga cccggaatcc gcagacgtca
     5281 gttcaggtcg atgaccacct gatcgttggg aatctcctcc atcctgtcca ggacgaacct
     5341 ctggatcttc ctcaaatccg acgccaactc gaggctgcaa tactgttcga aggtgcctag
     5401 aaagctgtcg accttccggt agagcttctt gtccaggtaa atccttcctt ccctttcctc
     5461 gacggctttc agggcaggca gggcttcgag gacgagctcg agggtctgcc agatcagggc
     5521 ggggcgatcc gagaggatgc gcagaagctc ctgtctatga tttagataca attgggtgaa
     5581 ttcccgagcc gtccggtccg gcacccggat ctcgtccagg attcccccga ggtttcgaac
     5641 gggagcgctc tcctccgtga tttccgtcgc cgtcgaagac tcctcgggcg cctgcgcgac
     5701 aaggtcggac tcgacggcga ttcggggttc ggcggcaaaa gatcccactg cggcaaccag
     5761 tcccaggaaa gccgggaaaa gagctcttcg tgcacgtacg gtcataaaat ttccttttcc
     5821 catccccgac ctattttgtt atggtgaccc gggggtcggt ctcgcatccg gtatgattcc
     5881 agacgattcc actcacggca ccagtcgagg agcgatgaga ttagtagcac ttctgaaacg
     5941 aaagcgattt tgggtcgctg cgtcggtcgg gttcttcatt ctggtttaca ctatcaaaaa
     6001 agcagacctt gaaaccctcc tcgagaactt gcaggacatt gggcgggggt gggccctggt
     6061 agccgttgcg gccgggctcg tcagctatct gtgcattgcc gccgtcctcc actacctgct
     6121 ccgaggaacg aaccatagcc tccccttttc gaccaccttt cgcatctccc tgctctcttg
     6181 caccctcaat tacatttggg ccatgggcgg gctcagcgga gtggcggcca aggtgtacct
     6241 tcttgccaag gaaaacattt cccccagcag caccctttcc atttccatgg ttcacggttt
     6301 tctgaccaac acggtggcgg tcctgttcat ctacctgggc tttttctttt tttattccaa
     6361 gaacaaaatg aacgccgagc agatggaact ggccgtcgtc cttcttctct tcgcctttgt
     6421 cctcacctgg ctgaccatcc aaaccatgat ccacgaatcg ttccgcaagc gcttatggca
     6481 gtttttgctc tatattgcaa acgccgtttg caggaagctg cgccgtcccc actggatcaa
     6541 gcaggatcgg gccgagagct tttttcggca tttcaatgcc aacatgaacc tcctggtgac
     6601 caatcggcgg attctgctgg cccccgccag ctacgccctg ctggactggt tctttatgtt
     6661 cctgtgtctg aaatatgcgt tctacgccgt gcactatccc gtggacaatt ggaccctgct
     6721 ggtgggtttt tccgtgggga tctttgccaa tctcttctcc ctgaccccgg cgtccatcgg
     6781 catcatggag gggtccatgg cggcttcctt ctactggatg ggcctcgatt acaatcacgc
     6841 ccttctcgcc accctgttgt atcgatttgc ctacttcttc ctgcccattt tgatcagcat
     6901 cttttactac aagcagttct tccggtcctc ggtggaagaa atcgaggtgg aagaggtccc
     6961 gaaaaaaacg tgacccgtct tttcaacctc cggagaccga acgccagagc attcgcaacg
     7021 gccagaggac caaaggctgc cagtaggttt ccagtcggat cagctggccc tggaagaagg
     7081 aatggttcag ggggatctcg gggttgtcct gccatttcag ggcgggaggt cgcagggacg
     7141 gaacgtacag gaagaggggc ggtgcggatt cccggggcag gacttgtccc ctccaggtga
     7201 attcatatcg gtcttcccgg gggtcccctt ccgggggttc ccaaaagcag atccgaaagc
     7261 gactccaggg ggtgaccggg tgttccgaga tcacggctcc cccaggccat gtctcgaagc
     7321 gggcaaaggg aagaggcacg gccagcagaa gcaccaggag ccacgtaacc cggcggtccc
     7381 acgcggcggg ccccccgtgt tcccgttgcc ggtcgtttgg aaacaaggag gtcatcggga
     7441 tcttcctttg aaataggtgg ctttccggcc gccgcaaagg ggggaagcgg ccggatcatc
     7501 ccaggggctg ggcgttgcgc aggtggtcca gccagatttc catgaccccg ggattttcgg
     7561 ccagcccctt gagactgatc gagcactcga ggccccggtg ctccaggatg gacttccaac
     7621 tctcggggtg gtctcccgcc atgtcggttc gaatgtgttc cccgggcgtc agcatgaagg
     7681 gaagcaggtg cactttgcgg agccttcccc ggtccaggcg gggcaagatc tgcgccaggg
     7741 acggaaaccc ctgggtggtg gctacgaaga cgttctcgtc ttcctccccg agggcaaagt
     7801 tcatggcggc ataaatcagg tctgcgggat ggtgggcgct cccatgcccc atgaatagga
     7861 cggcctcctc ctcggggttg cgttcggccg gaaggccctc gagaaggcat tggaccaccc
     7921 gccggagatc ttcgtaagaa gagagcagag gtcgtgtcag gcgaatttcg ggaagaccgt
     7981 gcttcgcccg atggaagccc cgggccgtgc gggccaggtc atgaaattcc ttcccgggga
     8041 tgacgtggag agaccagagg tcgatccgtt cgaatccttc ttccgcaaat cgggccaggg
     8101 ccgcttccgg ggagtcgatg aacgtccctt cctcggccag ctttcgacgc acgaccccgg
     8161 atgtgtacgc ccatcggaca acccggtcgg gaaaatgctc ccgaacgggg gatgccacgg
     8221 accccaggac cgcccgggcc tccgggaccc gggttccgaa ggccgtgaga agaattccgg
     8281 ttttcataag ccgatggtcc cttgttccca tcccccgcag caatccgggg cagggtatgg
     8341 ggctgaatcc atgattcctg ctccaggagg tttcatccat gacggtggac gaagtacgaa
     8401 aacgattgga cgaaatcgag caactggcgg gggatctgct cgagaccccc gaagacgaac
     8461 gcatcgggat ttacagggcc atggaccctc tgtttctgca actcgaaggt tggtggcgcg
     8521 aatcccccga tccgtccctc ctcgagcaag tcgaagagct caagctccgc atcctggtga
     8581 cggcgcggct ccaagagccg gatggcgagt ccgacgagag ccgccacctt tgggcactcg
     8641 aaattctcga atcccttcga agtgccgtcg cccggtgatt gggtcctgcg caggtcggct
     8701 tcccaatccc atgggcgagt cctcggcctg ggaatgcggc cgtaggggac tggacccggg
     8761 gtccgcggcg ggattccgcg ggatttcccc gaaggcgcct gggcccgccg ggaccccccg
     8821 cccggtcact tcaccgcctt gaaagtgacg ctgtccaggc cggtattgcc gttggcggga
     8881 tcgtacgcgt aaacgatcgc ctcgtactcc ccggtttctt cgatcttcaa gtctcctgcg
     8941 aaatgactgg gtgtcccttc aaacttcagg ggaaaaagcc ccatgggttt tccgtctttc
     9001 ttgagaatgg cggacacttc gaatttctcg gagtcccaca agccgcccgg cttgatgggg
     9061 caccctcaca tgagcgccgc gtaggcccgg atgggtaccg tgttgggcag cgattgaacc
     9121 gaggcgttgg gagacgggga caggaccttc accgcaaatc cgggcagctc gatcatccaa
     9181 ccgtcccctg cggtgatgtg tttgccggga aggacccact gggtgttcga tgcgcggttt
     9241 gcggcctgga gtccggacag gggacccatg gcttccacct ggaggagccg gggctcttcc
     9301 aggtcgatga ccgcgaggaa ttttgcggag gtttcatccg aaagcgggac cccgcgccgg
     9361 tggggtgttt ccatgatgcg ctcgttgctt ccggtagagc ctgccgtgcg tcccttggca
     9421 aggagttctc ccgtgtgggc gtccttgatg gtgaccaggg cgcctcccat gttggttccc
     9481 aggaacttgg catccttggt ttttacccgc acgatgattt ccgtgggcac ggcccatgcc
     9541 gccgatgcgc tcaacatccc cagcaagagc cccaaaccca atagcctgca caccttcccc
     9601 atgacggccc tcctcccggc atttcttccg caacaccccg ttctttcggc caggacctgc
     9661 gagaccggtc ctctcatcca tcggagaatt gtactcgggg cactttccgt ttacaatagc
     9721 cgggcacctt ggacctcgag ggtcaccgga ccccgaggcc ccacgaaccc cctgcacggt
     9781 ctttccctgc aatcttgccc ggttttcgtc acgagtcggc tgaaggtcga ggaacggcag
     9841 ggttcatgaa accgggagat ttgtcatgtc gaacgttcgt tcgtttacca ggttgactcc
     9901 gcaggaagct gcgctgcaca tttcccacgg aaataccgta gccttcagcg gttttgccaa
     9961 tgcgggagcg cccaaggcga tcccccgggc cctggccgcc agggcccgag agctccatga
    10021 ccggggagag cccttcaaga ttcgagtcct caccggtggc tccgggggcg ccgacatcga
    10081 tgaagccctg agtcgggccg aagccgtttc ctggagggcc gccttccaga gttccccgac
    10141 cctgcgcaag cagatcaacc ggcaggaggt ggaatatgtg gacatgcacc tgtcccatgt
    10201 tcccaccacg gtttccgccg gttttttcgg gaaggtggac gtggcggtgg tggaggccgt
    10261 cgagatcact cccgacggga gggtctacct gtccacctcc ataggggctt cccccacttt
    10321 tctgcggtgc gccgaacggg tcctgatcga aatcaacgcc tcccattccc cgaggctccg
    10381 ggaaatgagc gacgtgatca tcctttcccc tccgccccat cgcaacccca ttcccattca
    10441 gggtcccctg accaagttgg gctggtccta cgccgtggtg gaccctcggc gagtcatggg
    10501 agtggtggaa aacaacgagc cggacgatgt gcctcctttc gaccaacccg gggccgccag
    10561 cgagcagatc gccgaacacg tggtgcggtt tcttttcgat gaaatgcggg tcggacgaat
    10621 ccccgagagc ttcctgcccc tccaggccgg ggtgggcaac atagccaacg gggtcatggc
    10681 ggccctgggc cggaacccct acgtgccccc gttcaagatg ttttccctgg tcctccagga
    10741 ctcggagatc gggctcatgg aagaggggaa aatcaccggg gccagcgcca ccagcctcac
    10801 cctcagcccg gaagtcctcg accgggtgta ccgccacatg gacttcttcg tcccgaagat
    10861 cgtcctgcgc ccccaggaga tcgccaatca ccccgccgtc gtgaggaggc tcggggtcat
    10921 tgccatgaac gccgtcctcg aggtggacat ctacgggaac gccaactcgt cccatgtcta
    10981 cggcaccgat gtgatgaacg gagtgggagg cagcggagaa ttcatccgca acagctacat
    11041 ctccatcatc atgtgcccgt ccgtcgccaa gggagggcgc atcagctcca ttgttcccat
    11101 gtgtcctcac atcgacagca acgagcattc gatccagatc gtggtgacgg accagggcct
    11161 ggccgacttg cggggcctgg gtcccatgca gcgggccaaa gccatcatcg aaaactgcgc
    11221 ccatcccgcc taccgggaga ccctgtatcg atacatcgaa gaatcgaggc tgggtcacat
    11281 tcgccacaac ctgcgaacct gcttcgagct ccaccgcaac ctcgcccggt acggtgcgat
    11341 gcttcccgac cttgagcttt gggatgaggg ggagcaacca aaaccggcgt cccgttaagc
    11401 cgagcggcat gaacgggacg gcccatggat cggccgtccc atagattcag gagcacagga
    11461 atgccttttc cgattctctc ccccgaggaa gccgcagccc acgtctttga cggtgcaacg
    11521 gtcgccttca gcggattcac caatgcgggc gccgccaagg tgatccccag ggccatcgcc
    11581 caatatgccc ggaacctcca cgacggtggc aagcctttca agatccgggt cctgacgggg
    11641 gcctcgagcg gggccagcat cgatgaaccc ctggcccggg cggatgccat gtcctaccgg
    11701 gccccctacc agagcggtcc tcggttgcgc acccagatca atcgacagga agtggagtac
    11761 gtggacatgc acctgtctca cctcccccag acggtgctca gcggttttca cggaaagctg
    11821 gatttcgccg tgatcgaagc caacgaaatc actcccgacg gccgcgtgta tctcacctcc
    11881 tccatcggtg cctcgccgac ctacctgaag tacgccgatc gagttttcat cgagctcaac
    11941 gactaccact ccatgcgcct gcgggaaatg gccgacatct tcatcctgcc ccctccgccc
    12001 caccgatccc ccgttcccct tcacgagccc cttacccggg tgggattgcc ttacgccgtg
    12061 gtggaccccc gcagggtcct gggagtggtg aaaacctcgg aacccgacca agtcccggac
    12121 tttgccccct cggatgaaaa ggggcgccgg attgccgagc acgtggttcg cttcctggtg
    12181 gacgagatgc gggcgggaag gattcccgac gagttcctgc cgttgcaggc cggggtgggc
    12241 aacgtggcca acggggtcat ggcgggcctg ggcgacagcc cggacattcc ccgcttcaag
    12301 atgtatacgg aagtgttcca ggattccctg gtggatctca tggagcgggg aaagcttctg
    12361 gcggccagca ccaccagcct cacgctgact cccgcagtcc tgcgcaagat ctacgacaac
    12421 atggactttt ttgtgccccg gatcgtactt cgtccccagg aattgtccaa tcatcccggg
    12481 gtgatcacgc gcctgggggt cattgccatg aacacggccc tggaagtgga catctatggg
    12541 aacgtgaatt cttcccacgt cttcgggacg gacatcatga acggagtggg aggcagcgga
    12601 gagttcaccc gaaatgcgta tctttccatc tatatgtgtc cgtcggtggc tcggggaggc
    12661 aagatttccg ccattgtgcc catgtgcccc cacgtggaca acaacgagca ctccgtccag
    12721 gtggtggtga cggagcaggg actggcggac ctgcgggggt tgggtcccat gcagagggcg
    12781 cagaccatca tcgaacggtg cgctcatccc ctctatcgcg actatttgca ccgatacgtc
    12841 gaaaggtcgc gggtggggca catccgccac gacctcgagc tttgtttcga acttcaccga
    12901 aacctcatgg agaccggatc catgctcccg gaccgggacc tttccgctgc ggacgtttcc
    12961 tgacaggcga tatccgggac ccctcgcccc aaacatccgt tcccctcggg ttcgctccgg
    13021 ggaaggcgcc gggcgtcgag ttcctcaccc ccgggaaaac cggggattcg ggttttcgta
    13081 tccggaagga agcggaacgc cgggtattgt gaagggagtc tttgaacccc cgttcccacc
    13141 cgctttgccg gtcacgtctc ttgacccgcc gggattgctt tgctagaaga aagcccttcc
    13201 ctggttccct cgatgcgcgg aagagtaaag acacggaagg agctttcctt ggcaaccaga
    13261 aagatcctct tgggcaacga agccatcgcc cacggaatcc tcgagtccca ttgcacggtc
    13321 gcgacctcct acccgggcac tccggcctcg gaggtcctgc gaaccctgat tcgccttcgg
    13381 gaggagcatg ggctggacct tcacctggaa tggtcggtga acgagaaggt ggccttcgaa
    13441 gttgccctgg ccaacagcta cgtggggcga cggtcggcgg tggtcatgaa gcaggtggga
    13501 ctgaacgtcg cggcggaccc gctcatgagc gccgcctaca cgggagtcaa aggcggcttt
    13561 gtgctcgtgg tggcggacga tccgggacct cacagctccc agacggaaca ggacagtcgc
    13621 ttctttgcca tgatggccaa ggtccccgtg ttcgatccct cttctcccgc ggaagcccgg
    13681 gaattgatct gcaaggcctt cgagctgtcg gaaaaatacg agatccccgt gatgctgcgg
    13741 cccaccaccc gggtctgcca cgctcgcgag gacatggaaa tctcggccgt gtcccccgag
    13801 gctccctttc ccctcttcga aaagaacccg gcgcgatggg cggcaactcc caagttccga
    13861 ctcgagctcc accgggttct caatgagaag atccatcggc tcctggagga aaccgaccgc
    13921 cctcctcgtc tggtaaacga agactccctt ccctcccagc ccgatccgca ctcccggagg
    13981 aaagaaagca ggagttgcct ggtggcttcc gggacggccc tggctcatct ttgcgaggtg
    14041 ctccgcgacc ttgatctgtg ggacaaggtg cccctctacc aggtgctcat gccctaccct
    14101 ctttcctccg ccttccgtga aacccttctc gagcgctacg agcggatcct ggtcctcgag
    14161 gagacctatc ccgtcatgga gttgcagttc caggacgcgg ccaaggtcca tggaaggaga
    14221 aacggggtcg tcccttccca gggcgagctt cttccggaga ccgtggaaaa gatcctccgt
    14281 gagttcctgg ggctgcccgc cccccgggaa atccaagtcc cctcgatccc cggcagacgc
    14341 cccactctct gtgccggatg cccccatcgg gccacctttt tcgccatcaa gaaggcgttt
    14401 cccaagggca tctatcccgg ggacatcggc tgctacaccc tggggctgaa cctcggggtg
    14461 gtggacacgg tcctgtgcat gggggcctcc gtcagccagg ccacggggct gtacttcgcc
    14521 caccggtcgg gccgcaagga cgcaccggtc atttgtgcca ccatcgggga ttccactttt
    14581 tttcactcgg ggattccggc cctggtcaac gcagtggttc aggatgcccg gtttgtcctg
    14641 gtgatcatgg acaacggcac cacggccatg acgggccacc aggtgaaccc ctgcaccggc
    14701 gtggacgccc tggcggtggc ggtcggcaac gtgcacatgc tggagggccg ccaggcgggg
    14761 ctggacctgg agctgatccg gaccctgtgc cggcgcatcg aggttcccct ggtgctgcac
    14821 ggcgggaccg ggatccggcc cgcggcgctg cgggaggcca tccagttggg gat
//
LOCUS       DAOOVC010000098        14852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2696_length_14852_cov_5.809488, whole genome shotgun sequence.
ACCESSION   DAOOVC010000098 DAOOVC010000000
VERSION     DAOOVC010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 14852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14852
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2696_length_14852_cov_5.809488"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PLM79_12245"
     CDS             <1..1012
                     /locus_tag="PLM79_12245"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV87127.1"
                     /translation="FVCGEETALIQSIEGKRGIPQYRPPYPVQSGLFGRPTVINNVKT
                     LSSVPLILAKGGRWYSAIGAQNSPGTAIFSVVGNVKHPGLVEIPMGTTLRTLIFDICG
                     GIPKKKRFKAVQIGGPSGGCLPESFLDTSVDFDSLVQTGAIMGSGGLVVMDEDSCMVN
                     VARYFLEFTQKESCGKCTFCRIGTRHLLNTLNRITRGQGKEEDLALLESLSEDIKEGA
                     MCGLGKTAPNPIITSLRYFREEYEAHVRERRCPAKMCRAMTAFYIDPEKCSRGCEVCV
                     GCCPVEAIFSAKDRKKAVDQSRCVKCGECAVVCPPEYDAVRKVSPPELAPVVERPQTR
                     TK"
     gene            1009..1644
                     /locus_tag="PLM79_12250"
     CDS             1009..1644
                     /locus_tag="PLM79_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOV87128.1"
                     /translation="MIELTIDGKPIRAQEGKSLLRVCLENGIYVPNLCFLEEMTNPPA
                     SCRLCFVDVQGEKRPVPSCKVQPKPGMVVVTDTPRVRELQRSALRLLLSVHRAECRQC
                     GSNRKCELQRMVKFLGGVRLKPRRYENLARELPPDQDHGVVKYRLQRCVLCGRCVYVC
                     AKLHPIPVLTFAKRGFDTIISAYGREDARELCRECRACVRVCPVSALEIGE"
     gene            2055..2864
                     /locus_tag="PLM79_12255"
     CDS             2055..2864
                     /locus_tag="PLM79_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV87129.1"
                     /translation="MFKRCVLVGVLAVAATVIGWSLQATQVCAVEGIHYDGSSQIYWA
                     LVKDSMEAFTKETGIKVTAEDRKTQDAVPSLVSGRCNVGGLARKMKLAEKSQSQDLTE
                     TLIARDHIAVFVPAGSKAEEVTKEQLRKVFSGQITDWKDLGDDPGPIQVVIPQTKTTC
                     NRNFTELVMGDAPFAQSSVITETAGAVLDAAKGKRAISFISFGAVSKTADFKVLKVDG
                     KKPGENGYPIAQEMYFATMGPPSGDVKKYVDFFLTGSGKEIILKQGLLTVQ"
     gene            3045..4910
                     /locus_tag="PLM79_12260"
     CDS             3045..4910
                     /locus_tag="PLM79_12260"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV87130.1"
                     /translation="MRVRAKLGTRIILQTMILSLVPIFVVTGLNVMNAKNEMEKLVRQ
                     DFTNMIGFVWEIMEAYEALVKEAEIGEEIVLILQAREQEKNFIIKEDEASIKAWHAIM
                     GEIKKSPVYVGDVPASLKHYEAVFEKFSRGMLANIGDLAQAGQALEAEIKKTVKTVKY
                     AEYQEAIRTKVIGPKNSDGTRDLNKGIRVGATGYIFFLKPDGKLMGHPTLESKTLANA
                     ELARIIGQRKDGYTTYRENGRDKIAFFKYYAPWDWIVVIDAVQSEVMNVSNIIKVGLS
                     VAGTFTLLVAVLNFFFVRSITRPIHNLLDGLSEGANQVASASSQVSTASQSLADGASA
                     QAASLEETSSSLEEMSSMTRQNAENARQANDLTRDTNRVVNQANSVMTELTRSMQEVS
                     TASQETSKIIKTIDEIAFQTNLLALNAAVEAARAGEAGAGFAVVAEEVRNLAMRSAEA
                     ARNTSVMIEGTVRKIQDGVKLLGRTNEAFQEVARHTDQVGQLVERIAAASNEQAQGIE
                     QVSRAAAEMDRVTQQTAASAEESASASAQLSAQAEQMLSSLDDLLKLIGRNGNGNQES
                     RGAGPRDEEPRAGAQRVYAAVRKPVAASGELPDAKTGCAALPSSRPTPGRKLIDG"
     regulatory      5315..5400
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5655..6548
                     /locus_tag="PLM79_12265"
     CDS             5655..6548
                     /locus_tag="PLM79_12265"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV87131.1"
                     /translation="MLRDLSNEEIKALHHELLLKDILAVAERLPPFPDVAWKVIPLIR
                     RMAPVQEIEAVIRYDQAITARVLALSQSAYYGRKQSAATLKEAVLILGAQRLVQVILV
                     ACAARYFQGRDPGCDVEERKLWEHSVATALTAEILARRLSHRKVLAVYTASLLHDIGK
                     TVLDLYAKMYLHTSLRRMRGKGAGFLEAERNALGIDHQELGVMIARRWKFPPEVVAAI
                     GHHHCPLKAPSDLDIASIVYAANRMTHLRTSDEDTVELKQLESDPVFIGLGLSGNAIE
                     DIEVAAREAMKEVVSFLTSPW"
     gene            complement(6729..8270)
                     /locus_tag="PLM79_12270"
     CDS             complement(6729..8270)
                     /locus_tag="PLM79_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87132.1"
                     /translation="MILLVHPPTTKACEPPAGIARLAGALRSHGVRFRVWDANLGGLV
                     DLLERIPPDGDRRTRRAVKNRTLFLERLRSPAGYQSLDRYKQAVGGLDHLLKTAALPF
                     GVRLGLANFEHTRRLPVRSADLLDAAETPRDNPYYSHFSRKLRQILEEDSPSVAGFSL
                     SYLSQALCTFAMMGFLRREAPGIRIALGGSLLTSWVKGPHWKNPFGGLVDHCIPGPGE
                     DRLLRLAGIDPEPGRIYAPDYSFAGPADYLSPFPVIPYSASSGCYWKRCAFCPEQAEG
                     NPYRPIPSDRVLQDLGLLTRAGQTALVHFLDNAMRPSLLEKLMRRPPGVPWYGFARVT
                     SALTDQSYCRDLKRAGCVLLKLGIESGDQEVLDRENKGIRLEDASGALRTLHEAGIGT
                     YVYLLFGTPAESYPSALRTLDFTVRHGNEIDFLNLSIFNLPVGNADSSLSTAPHYEGD
                     LSLYTGYEHAGGWDRKRVRQFLDREFRRHPGIAQILRREPPVFTSNHAPFFVRDRDLP
                     NPAGR"
     gene            complement(9044..13309)
                     /locus_tag="PLM79_12275"
     CDS             complement(9044..13309)
                     /locus_tag="PLM79_12275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOV87133.1"
                     /translation="MVRPRGKKSRFGFLLALGSLLAFQMTYFSAAPPAPLAETWTVYG
                     EAGMKATVEYHFSARAELDVNQSNEVWRIYRVMGDGSPQFSLTAKVLEGGTPPGGEFS
                     QFAQLNLSSTLPNGNTHDIWISPIYDGQSGTLSGGLPMGFYYDQWVDLAPHDGFMWFA
                     AQYYLDPACGATLECNRDHAGGPCENPGTPFTWPPQGAGSSASGSSGQRSACSDCRGG
                     CAAQGLPVWYVNLELLNLVIQDTDFRYDSFGRTVAMQRTWNPPVPNQDGMFGRGWSFA
                     YESSAVSYNVAQGEAGTDPMGLVFLNLGTGRRFQYTASVYEGVGTGQVTIRYVCRNTM
                     DNPRPLIVTGYLDEATRAERFIVRDQDAKITYYYGQTALGDVNGGRAHLLTSIEDRDG
                     NLITLTHGANDRLERLTDASGRETEFAYDGNNHCTAMTTFDGKQAFYHYDAAGNLVET
                     VDLAGNASTYTYDAKGHVTELATNGNGTTFTWTDASGYWKVASATNSLGHMERYEEVN
                     GGVRVIDPRGGVTTCTVTPDGTGGYSRSVTDRLGTTTTIFYTNNYASPYNLPPGLPVL
                     CRRSDLSVPLWTADYDPVGNLVTFTDAMGASTFFTYDDRRNVTGITDAVNHAWRFAYD
                     GRDRLLEAVSPTGGRLSLLWDALGNLSQITRPSGATYLLGYDPHGNLAAITNPLGQKT
                     TVAYDATGLNPASIVDPLGRTTALTFDANRHLTQVTNPDGTSRHYGYDCCALTSYQDE
                     NGQTTVVERDSLQRPLSVTDPLGNVRSFDYDGNGNVVAWTDPLGQISTHTYDAANRWV
                     GWTNPVGAIVQMTYDTRGNLTSLTDPGSGTTGFTYDNNDHPVSVTDPLAWTQALSWNL
                     LGQFTLLTNARGRAVGFEYDEDGRLVARKHDGSPFAGYAYDNDGNLTTVVDATGTTTY
                     VHNAQGRVIEVQYDIGRATLAYDAAGNLTSITYPSGLRTDYTYDARNRVIAMSWGDHA
                     ITFGYDAVGNLLSEFRSNGVNSMYTYDANNRSTGIEHARGAEVIAGFQYSRNPVGSII
                     EETNRLPLAPSFKEKTSVMEYNDANQLVSMDGNPCTYDQDGDLADMGDTFSATYDPEN
                     RLTAVTQGGNQKIYSYDGHGNRRSVEDQAGISREYYDPFGRLLFETDASGSITAQYVY
                     DGMRLVAMMKGLDAYFYHFEKTGNTIALTDRTGAVANAYAYEPFGAIIASSGQVRNPF
                     TFVGAFGVRDEGGGIYLMRNRYYDAVTGRFLQKDPIGFMGGINLYAYVGNNPVDRMDP
                     QGTAGPFDAINSLYQAKKIGESCPVFGPVAAAFASAQIGCDQEIPDSVKAEARKKAIQ
                     SSEECSRNQLPQQWVFRLYVFLVLQALEQGSTLPSGLHGERRSDQWLPTGTWGGVSDV
                     FEDLIRMPYGNTPNEKAADWISRFLDTVVEGKGFERILYEMGH"
     regulatory      13855..13940
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(14264..14395)
                     /locus_tag="PLM79_12280"
     CDS             complement(14264..14395)
                     /locus_tag="PLM79_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87134.1"
                     /translation="MKDLSGKTRDSLLILLMHLPTAKACEPQGGGARPAGALRSRGV"
     gene            14560..>14852
                     /locus_tag="PLM79_12285"
     CDS             14560..>14852
                     /locus_tag="PLM79_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOV87135.1"
                     /translation="MEYDEGTMGRIFTLRLGDGDRLPDTIEVFAQAKRIRNALVFYLG
                     GAADGSRLVVGPEENRGDAVVPMIHTLRGAQEVLAVGTLFPSESGEPVLHMHAA"
BASE COUNT         2971 a         4320 c         4673 g         2888 t
ORIGIN      
        1 cttcgtttgc ggagaagaga cggccctcat ccagtccatc gagggcaaac ggggcatccc
       61 ccagtatcgg ccgccttacc cggtccaatc cggcctcttc ggcaggccca cggtcatcaa
      121 caacgtgaag accctgtcct cggtgccgct cattctcgcc aagggcgggc gctggtatag
      181 cgccatcggg gcccagaaca gtcccggcac ggcgattttc tccgtggtgg gaaacgtcaa
      241 gcacccggga ctggtggaaa tccccatggg gaccaccctt cgcaccctca tctttgacat
      301 ttgcggtggg atccccaaga agaaacggtt caaagcggtg caaatcggag gcccttcggg
      361 gggatgcctt cccgaaagct tcctggacac ttccgtggac ttcgattccc tggtccagac
      421 cggggccatc atgggctccg gcggcctggt ggtcatggat gaagacagtt gcatggtgaa
      481 cgtggcccgg tactttctcg agttcaccca gaaggaatcc tgcgggaaat gcaccttctg
      541 ccgaatcggg acccgtcacc ttctcaacac tctcaaccgg atcacccgag ggcaggggaa
      601 agaagaagat ctcgccctgc tcgaatcgct gagtgaagac atcaaagagg gagccatgtg
      661 cggtctggga aagacggctc ccaaccccat catcacgtcc ctgcgatact ttcgggaaga
      721 atacgaggcc catgtccggg aaaggcgatg tccggccaag atgtgccgtg ccatgaccgc
      781 cttctacata gacccggaaa agtgctcgcg gggatgcgaa gtctgtgtgg gatgctgccc
      841 ggtggaagcc atcttctccg ccaaggatcg gaagaaagcg gtggatcaga gccgatgtgt
      901 gaagtgcggc gagtgcgcgg tggtctgccc tcccgaatac gatgccgtga gaaaggtatc
      961 gccgcccgaa ctggctcccg tggttgaaag gcctcagacg cgaacgaaat gatcgaactg
     1021 accatcgatg ggaagcccat ccgggcgcaa gagggaaaga gcctgctccg ggtctgtctg
     1081 gaaaacggca tctatgtccc taatctatgc ttcctggagg agatgacgaa tccgcccgcc
     1141 tcttgccgct tgtgctttgt ggatgtccag ggagaaaagc gaccggtgcc ctcgtgcaaa
     1201 gtccaaccca agcccggcat ggtggtggtg acggacactc cccgggtaag agagctccag
     1261 cggtctgccc ttcgactgct gctttccgtc catcgggccg aatgtcggca gtgtgggtcc
     1321 aaccgaaagt gcgaactgca gcgaatggtg aaattcctgg gaggggtgcg tctcaaaccc
     1381 cgcaggtacg aaaaccttgc ccgggaactc ccgccggacc aggaccacgg cgttgtaaaa
     1441 taccggcttc agcgctgcgt gctctgcggc cgatgcgtgt acgtgtgcgc aaagctgcac
     1501 ccgatcccgg tgttgacctt tgccaagagg ggcttcgaca cgatcatcag cgcttacggg
     1561 cgggaggatg cccgggagct ttgccgggaa tgtcgcgcct gcgtccgcgt ctgcccggtg
     1621 agcgcgctgg agatcgggga ataagggatg ggccgggggg cgaggggcta aaggttgatt
     1681 ggttagttgg cgaggggctg gttggttttg ctcgtttaag tttgtgagct acgttgcggc
     1741 gcctctctct ctttaaggtt ccccccaaag tctagacaaa aaacgggtca acctaagctt
     1801 tttttcacct cctccgacca gcctttctga acgggctgtg ttatgcttcc tctgcccacc
     1861 caccctacac cattccccag cctcccggct tctcgtccca aattacttct caagcttttg
     1921 cagtcccgaa ccgatggata ggacatgcga catttgcccg caggcgggga atatcgaggt
     1981 tccggttttt cggagcgact cgctgcggga ttgtgacgag gcaaaggttg gggattatgc
     2041 agaagggggc aggtatgttc aagcggtgcg ttctggtggg tgtgttggcg gtggctgcga
     2101 ctgtgattgg ctggtctcta caggcgacgc aagtctgcgc ggtggaagga attcattatg
     2161 acggatcctc tcagatctac tgggccctgg tgaaagattc catggaggcc ttcaccaagg
     2221 aaacgggaat caaggttacc gccgaggatc gaaagaccca ggatgcggtt ccgtcgctgg
     2281 tttcgggcag gtgcaacgtt ggcggcctgg ccaggaagat gaaactggcc gaaaagtccc
     2341 aaagtcaaga cctgacggaa accctcattg ccagggatca tatcgccgtt ttcgtccctg
     2401 cgggcagcaa ggccgaagag gttaccaagg aacaacttcg aaaagtcttt tcaggccaga
     2461 tcaccgattg gaaggacctg ggcgatgatc ccggccccat ccaggtggtg attcctcaaa
     2521 ccaagaccac ctgcaaccga aatttcaccg aattggtcat gggggatgct ccttttgccc
     2581 agtccagcgt cattaccgag acggcggggg ccgtgttgga tgcggccaag ggcaagcggg
     2641 ccatctcctt catttcgttt ggagccgttt ccaagacggc cgatttcaag gtgctcaaag
     2701 tggacggcaa gaagcccggg gaaaacggct atcccatcgc ccaggagatg tactttgcca
     2761 ccatgggacc gccgtcgggg gacgtgaaaa agtatgtcga ctttttcctt acgggatcgg
     2821 gaaaggagat catcctcaaa caaggcttgc tgacggttca gtagctgaga gagggccttg
     2881 ggtcctcccc ggtgcctatc ggttttgagg gaagtgtccg ggttgtccct cctctccttg
     2941 tccaaacgcc ggggagagga ggggatcgga gaggggggga cttcatccga tcgcctctgt
     3001 tgacgacgtt cccgttccct ccctccaggt gcaggaggca gaccatgaga gtgcgcgcaa
     3061 agctgggtac gaggatcatc ctgcagacca tgatcctctc cctggtcccg atatttgtcg
     3121 ttacgggcct caatgtcatg aacgccaaga acgagatgga gaaactcgtt cgccaggact
     3181 tcaccaacat gatcggcttc gtctgggaga tcatggaagc ctacgaagcc ctggtcaagg
     3241 aagccgagat cggagaagag atcgtcctga tcctccaggc ccgtgagcag gaaaagaact
     3301 tcatcatcaa ggaggacgaa gccagcatca aggcgtggca cgccatcatg ggggagatca
     3361 agaaatcccc cgtgtatgtg ggggatgtgc ccgcaagtct caagcactac gaagcggtgt
     3421 tcgaaaaatt cagccggggc atgctggcca atatcgggga ccttgcccag gcgggacagg
     3481 ccctggaagc cgaaatcaag aaaacggtca agaccgtcaa gtacgcggaa taccaggagg
     3541 ccatccggac caaggtcatc ggtcccaaga attccgacgg gactcgagac ctgaacaagg
     3601 gcatccgcgt gggagccacc gggtacatct ttttcctcaa gccggacggg aagttgatgg
     3661 ggcatccgac cctcgaatcc aagaccctgg ccaatgcgga actggcgcgg atcatcggtc
     3721 aacggaagga cgggtacacc acctaccggg aaaacggtcg ggataagatc gcgtttttca
     3781 aatactacgc cccttgggat tggattgtgg tgatcgatgc cgttcagagc gaagtgatga
     3841 acgtgagcaa catcatcaaa gtggggttga gcgtcgcggg gacctttact cttctcgtcg
     3901 ccgttctcaa cttcttcttc gtgcggtcca tcacccggcc cattcacaat ctcctggacg
     3961 gcctgtccga aggggccaac caggtggcgt cggcatcttc ccaggtctcg accgcaagcc
     4021 agtccctggc cgatggagct tccgcccagg cggcgtccct cgaggagact tcctcgtctc
     4081 tcgaggaaat gtcgtccatg actcgccaaa acgcggaaaa tgctcggcag gccaacgatc
     4141 tcacccggga taccaaccgg gtggtgaacc aggccaacag cgtcatgacc gagttgaccc
     4201 gttccatgca ggaggtttcc acggccagcc aggagacctc taagatcatc aagaccatcg
     4261 acgagatcgc ctttcaaacc aacctgttgg ccctgaatgc cgccgtcgag gcggctcgtg
     4321 ccggagaagc gggggccggg tttgcggtgg ttgccgagga ggtgagaaac cttgccatgc
     4381 ggtcggccga agccgccagg aatacctcgg tgatgatcga aggcaccgtc aggaagatcc
     4441 aagacggggt caaattgttg ggaaggacca atgaggcctt tcaagaggta gcccggcaca
     4501 cggaccaggt cggccagttg gtggaaagga ttgcggcggc ctccaacgaa caggcccagg
     4561 ggatcgaaca ggtgagcagg gccgcggcgg aaatggaccg ggtgacccaa cagacggcgg
     4621 cgagcgccga ggaatcggcc agtgcgtcgg cacagctcag cgcccaggcg gagcaaatgc
     4681 tgtcgagtct ggatgatctg ttgaagctca tcggccgcaa cgggaacggg aatcaggaaa
     4741 gccggggcgc agggccccgg gatgaggagc cccgggccgg ggcgcaacgc gtctatgccg
     4801 cggttcggaa gcctgttgcg gcatcgggag aacttccgga cgcaaaaacc ggctgcgcgg
     4861 ccctgccttc ttcccgtccg actcccggga ggaagctcat cgacggttag aaaccctggg
     4921 acggtatgga agcccaaagt ccccccccac ggggctccgg ggccgccggg cggggacgat
     4981 tcgcctgagg gggattctcc aacgacgggg caagacgtgg atcgagtctc gacgggcgca
     5041 gacagaccac gccgacgccg tctcctacgg cggcgccggc acaaagtctc ttggaagtag
     5101 acgaacaccc gaagggggaa gtcgcggttt cgttgcacga ggagaggaaa ggcccgcccg
     5161 gaagggcggg tgcaccgact gcgcatgcag cggcggggaa aatttgggcg tcgagcaatc
     5221 gcttgacctc ccaagaagaa cctgcttaaa tgggtgccaa agcagtgaat ggtttcgaga
     5281 gtcgccgggg ttggaaggca gggctccggc aagggctcga taaaggcaaa ctgctcgaaa
     5341 gggtaggacg caaagcttct ggcctaagtc cccaacggat aaggtcgcag ggctgccggg
     5401 cgaatcgcat gagagtagat cctttcaaga gtatctccgg gtttcctccg cgtttttggt
     5461 tctcctttcc ctgcaatcgc tcgctttttc catggacaca ggtttgggtt gtccggatag
     5521 gcatccgttg ggtcgcggtt cttgcggaac catggaaccg tgtttcggcc tcgttgtttc
     5581 cgggggtccc ggcaaaatgg acggaaccct tcggaaccgc cgcaaaaccg atccggtcct
     5641 gtgaggagtg agcaatgcta agagacctca gtaacgaaga aatcaaggct ctgcatcacg
     5701 aactcttgtt gaaggacatc cttgcggttg cggagagact gccgccgttt cccgacgtgg
     5761 cgtggaaggt cattccgctc attcgcagga tggctcccgt ccaggaaatc gaggcggtga
     5821 tccgatacga tcaggccatc accgcaaggg tccttgcctt gagtcaatcg gcctattacg
     5881 ggcggaagca gagtgccgca accttgaagg aagccgtgct cattctgggg gcgcagaggc
     5941 tcgtccaggt catcttggtg gcgtgtgcgg cccgatattt tcaagggagg gatccgggat
     6001 gcgacgtcga ggagcgaaaa ctgtgggaac attcggtggc gacggctctt accgccgaaa
     6061 tcctggctcg caggctgtcc caccggaagg tcctcgccgt ctacacggcc tcgctgcttc
     6121 acgatatcgg caagaccgtg ctggacctct acgcaaagat gtacctccac acgagcctga
     6181 ggcggatgag gggaaaaggg gcggggttcc tggaagcgga acggaatgcc ctgggcatcg
     6241 atcaccagga gttgggagtg atgattgccc ggaggtggaa attcccgccg gaagtcgtgg
     6301 ccgccatcgg gcatcatcat tgccccctca aggcgcccag cgacctggac attgcgtcca
     6361 tcgtctatgc ggccaaccgc atgacccatt tgaggaccag cgacgaggat acggtggaac
     6421 tcaagcagtt ggagtcggac cccgtgttca tcgggttggg gttgagcgga aacgccatcg
     6481 aggacatcga agtcgccgca agggaggcga tgaaggaagt agtgtccttt ttgacctcgc
     6541 cttggtgacc gtggacagtc gcccagggga acccttcccc tggggccggg cgcccggcat
     6601 cccggcaagg ccacggcgat gggctcccgg ggaggatccg ccgggaacga ccccgccggg
     6661 gatcccttcc gcctgcttcg gggagaagtc acggcgcctc ccggcgcatc cggtcttccc
     6721 tcgagttttc aacggcccgc aggattcggg agatccctgt cccgaacaaa gaagggagca
     6781 tggttggacg taaagacggg cggctcccgc ctcaggattt gcgcaatccc cggatgtctt
     6841 cggaattccc ggtccagaaa ttgcctcacc ctcttgcgat cccacccccc ggcgtgttcg
     6901 tagcccgtgt agagagacag gtctccttcg tagtgcggcg ccgtcgacag ggatgaatcg
     6961 gcgtttccca ccggcaggtt gaaaatcgac aggttcagga aatcaatctc gttcccgtgg
     7021 cggacggtga agtccagggt gcggagcgcc gaaggataag actccgcggg agtcccgaaa
     7081 agcaggtaca cgtaggtgcc gatgcccgcc tcgtgaaggg tccgaagggc cccggaggca
     7141 tcctccaggc ggattccctt attttcccga tccagcacct cctggtctcc ggattcgatc
     7201 cccagcttca gcaaaacgca cccggccctc ttgagatccc tgcagtagct ttggtccgtg
     7261 agggcggagg tgacccgggc aaagccgtac caagggactc cggggggacg gcgcatcagc
     7321 ttttccagga gggaaggcct catggcgttg tccaggaagt ggaccagggc cgtttggccg
     7381 gctcgggtga gaaggcccag gtcttggagc acccggtcgg aggggatcgg ccggtacggg
     7441 ttgccctcgg cctgttccgg gcagaaagcg caccgcttcc agtagcaacc cgaagaagcg
     7501 ctgtacggaa tgaccggaaa cgggctcaaa tagtcggcgg gcccggcaaa cgagtaatcc
     7561 ggagcgtaga tccgcccggg ttcgggatcg attcccgcca ggcgcaggag gcgatcctcg
     7621 ccggggccgg ggatgcaatg atccaccagg cctccgaagg ggtttttcca gtgcggcccc
     7681 ttgacccacg aggtgagcag gctcccgccc agggcgattc gaatcccggg agcctccctc
     7741 ctcaaaaaac ccatcatggc aaaggtgcat agggcctggc tgaggtagct gagggaaaac
     7801 cccgccaccg agggggaatc ctcttcgaga atttgacgga gcttccggct gaagtgagaa
     7861 taatacggat tgtcccgggg ggtttcggcg gcatcgagaa ggtccgcact tcttacgggg
     7921 agccgacggg tgtgctcgaa gttggccagg cccagacgga cgccgaaggg cagggcggcc
     7981 gttttcagca ggtgatccag gccccctacg gcctgtttat atcgatccag gctctgatac
     8041 ccggcaggag aacggagacg ttccaggaag agggtacggt tcttgaccgc acgccgggtc
     8101 cgtcgatcgc cgtcgggggg aatgcgttcc agcaggtcca cgagtcctcc cagattggcg
     8161 tcccaaaccc ggaagcgtac gccgtgggaa cgcagggccc ccgccaggcg ggcaatgccg
     8221 gccgggggtt cacaggcctt ggtcgtgggg ggatggacga ggaggatcaa aagggaatcc
     8281 cttgtccttt ccaatgactc tttcatggcc gccgtacggg cgggcagccc ggaggcggat
     8341 tcccgcgtgt tccatctcct cgattcggaa ggcaagcgac aatgcccccc tgtccatcgg
     8401 gaccggcttt cccacaggac gtctccgatc cgtcaacaaa acctcttttc ccttctcctc
     8461 catcctgagg agtctgctca ggtttgcctt ggtttcccgg attccgacat gctagcttgt
     8521 catagcatgc ttcccgaatg tggttaccgt agacgctttc aagcaaggcc atggtagtca
     8581 cgttggaggg cagggccaag ccaatgcaaa tccgggatcg ccctgcccgg gcccaatccc
     8641 ggaatcgtct ttggggaagc gcatcgagca ggtcgtgaat accccggatg gggtctcctg
     8701 tgggttgctc atctccgctt cgatgggcaa gcaacccttt gaagttacac acaaggtgta
     8761 gggtagctta atgtgtaagg ggaggcctcc tatggcgaca aaactcgccg tccacgacaa
     8821 acggatcgcg gaagcccgcc tgatcggcag acatcccata agagaaggac gtggtctccg
     8881 aagcgctcct tgaatatacc cggcaccggc ccggcgaccc taggaggatg cggcagaacc
     8941 gcgggtaccc gaaccatgat ggttttcctc acttcttgaa caactcgggg tccggcttgg
     9001 cttgcaggcg caacaccatt cttccgggat gcactatcag cggtcagtgt cccatctcgt
     9061 acagaatgcg ctcgaagccc ttcccctcaa ccacggtatc aagaaacctg gaaatccaat
     9121 ctgccgcctt ttcgttgggt gtattgccat agggcatcct aatcaggtcc tcaaagacat
     9181 ctgaaacgcc cccccacgtc ccggtgggaa gccactgatc cgaccgtctc tcaccatgga
     9241 gaccggacgg gagagtactc ccttgttcca gagcctgcaa tacgagaaag acatagagcc
     9301 gaaacaccca ctgttgcgga agttggttcc ttgagcattc ttccgatgac tgaatcgcct
     9361 tttttcgagc ttctgctttc accgaatccg ggatctcttg atcgcatcct atctgtgcac
     9421 tggcgaaagc cgccgctaca gggccgaata ccgggcagga ttcgccgatt tttttcgcct
     9481 gatataaaga gtttatcgca tcgaatggac ctgctgttcc ctggggatcc atcctgtcca
     9541 ccggattgtt cccgacgtag gcgtagaggt tgatccctcc cataaacccg atggggtctt
     9601 tctgcaggaa ccttcccgtc accgcgtcgt agtaccggtt tctcatgaga taaatcccgc
     9661 caccttcgtc ccgcacaccg aaagccccga caaaggtgaa cgggttccgc acctgtccgg
     9721 aggaggcgat aatggcccca aagggctcgt aagcatacgc gttcgccacg gcccccgtgc
     9781 gatcggtgag ggcgatcgtg ttgccggttt tctcaaaatg atagaaatag gcatccagtc
     9841 ccttcatcat ggcaaccagg cgcatgccgt cgtagacgta ttgagccgtg atgcttccgc
     9901 tcgcgtccgt ctcgaacagg aggcgtccaa aaggatcata atactccctg gagattccgg
     9961 cctgatcctc cacgctccgc cggtttccgt gcccgtcata gctgtaaatc ttctgatttc
    10021 ctccctgcgt aacggccgtc aggcgattct cggggtcgta ggtggcggag aaagtgtcgc
    10081 ccatatctgc caggtccccg tcctgatcgt aagtgcatgg attcccgtcc atactgacga
    10141 gctggtttgc gtcattgtac tccatcacgc tcgtcttctc cttgaaggag ggggccaggg
    10201 gcagtcggtt ggtctcttct attatactgc cgacgggatt tcgactgtat tggaagccgg
    10261 cgatcacttc cgctcccctc gcgtgttcga tccccgttga ccggttgttt gcgtcgtatg
    10321 tgtacatgct gttgacgccg ttggaccgga actccgagag caggttgccc acggcatcgt
    10381 agccaaaggt gattgcgtgg tccccccacg acatggcgat cacccggttt cgcgcgtcat
    10441 aggtgtagtc cgtcctcaaa ccggagggat aggtgatgga ggtaaggttt cccgccgcat
    10501 cgtaggccag agtggctcgc cctatgtcgt attggacctc gatgacacgg ccttgggcgt
    10561 tgtggacgta ggtcgtcgtc ccggttgcgt ccacgacggt ggtcaggttc ccgtcgttgt
    10621 cgtaggcgta accggcgaag gggctcccgt cgtgtttcct ggcgaccaga cgcccatcct
    10681 cgtcgtattc gaacccgacc gcccgtcccc gcgcattcgt cagcaacgta aattggccca
    10741 gcaggttcca cgaaagcgcc tgtgtccagg caagggggtc ggtaacgctc actggatggt
    10801 cgttgttgtc gtaggtgaac cctgtggtgc cggagcccgg atccgtcaga gacgtgaggt
    10861 tccccctcgt gtcgtacgtc atctgcacaa tcgcgccaac cgggttcgtc catcccaccc
    10921 aacgatttgc agcgtcgtat gtgtgggtgg aaatctgccc gaggggatct gtccaggcga
    10981 caacgttccc attgccgtcg tagtcaaaac tgcggacgtt gcccaggggg tccgtgacgc
    11041 tcaagggccg ctggagcgag tcccgctcca cgaccgtggt ttgcccgttc tcgtcctggt
    11101 aggacgttag ggcgcagcaa tcataaccgt agtgccggga agtgccgtcc gggttcgtga
    11161 cctgagtgag gtgtcggttc gcgtcgaagg tcagggcggt cgtcctgccc agggggtcga
    11221 cgatcgaggc cgggttcagc ccggtcgcgt cgtaggcgac ggtggtcttc tgaccgaggg
    11281 ggttcgttat ggcggcgaga tttccatgcg gatcgtagcc aaggaggtat gtggcaccgc
    11341 tgggacgggt gatttgagag aggttcccga gggcgtccca caaaagggag agcctccccc
    11401 cggttgggct caccgcctcc aggagccggt cccgcccgtc gtaggcaaac ctccaggcgt
    11461 gattcacggc gtccgtaatg ccggtcacgt ttcgacggtc gtcgtaagta aaaaacgtcg
    11521 aagcgcccat ggcatccgtg aaggtgacca ggttgccgac cgggtcgtaa tccgccgtcc
    11581 agaggggcac ggaaaggtcc gatctccggc acaataccgg caggccgggg ggaagattgt
    11641 atggagaggc ataattgttc gtgtagaaga tcgtggtcgt ggttccgagc cggtccgtaa
    11701 cgctccggga gtagccgccg gtgccgtcgg gagttaccgt gcaggtcgtc acgccgcctc
    11761 tggggtcgat cacccggacg ccgccgttca cctcctcgta tcgttccata tgtccgaggc
    11821 tgtttgtggc ggaggcgacc ttccagtagc ccgaggcatc ggtccaggtg aatgtggtgc
    11881 cgttgccgtt cgtggccagc tcggtgacat gccctttcgc atcgtaggtg tatgtgctcg
    11941 cgttgcccgc caggtccacc gtctccacca gattccccgc tgcatcgtag tggtagaacg
    12001 cctgcttgcc gtcgaaggtc gtcatcgcgg tgcagtggtt gtttccgtcg tacgcaaatt
    12061 ccgtctcgcg cccggaggcg tccgtcaatc tttccagccg gtcgttcgcc ccgtgggtca
    12121 gggtgatgag gttgccgtcc cggtcctcga tggaggtcag gaggtgcgcg cgacctccat
    12181 tgacgtcccc caaggctgtc tgtccgtaat agtaggtgat cttggcgtcc tggtcccgga
    12241 cgatgaaccg ctcggcccgg gtggcttcat cgaggtaccc ggtgactatg aggggcctcg
    12301 ggttgtccat cgtgttccgg cacacgtagc gtatcgtcac ctggccggtg ccgacacctt
    12361 catacaccga tgccgtgtac tgaaacctcc ggccggtgcc gaggttgagg aacacgagcc
    12421 ccatggggtc cgtccccgcc tccccttgag cgacattgta gctcaccgcg gaggactcgt
    12481 aggcgaagga ccaaccgcgg ccgaacatcc cgtcctggtt gggcacagga gggttccacg
    12541 tcctttgcat ggccactgtc cgtccgaagc tgtcatagcg gaagtcggtg tcctggatta
    12601 ctagattgag cagctccagg ttgacgtacc agacggggag gccctgggcc gcgcatcccc
    12661 cccgacaatc cgagcacgcg gagcgctgtc ccgaggaccc ggatgcggag gagccggctc
    12721 cttgcggggg ccaggtgaag ggcgtgccgg ggttctcgca gggacctccg gcatgatcgc
    12781 gattgcactc gagggtagct ccgcacgcgg gatcgagata gtactgagcg gcaaaccaca
    12841 tgaatccgtc atggggtgca aggtcgaccc actggtcata ataaaagccc atcggcagtc
    12901 caccggatag ggtccccgat tggccgtcgt agatggggct gatccaaata tcgtgtgtat
    12961 tgccattcgg cagagtggaa gataggttga gctgcgcaaa ctggctgaat tccccgccag
    13021 ggggggtgcc cccctccagc acctttgcgg tcaaggaaaa ctgtggagac ccgtctccca
    13081 tgacccgata gattctccag acttcgttag actgattcac gtccagctca gcccgtgcgg
    13141 agaaatggta ctcgaccgtt gccttcatgc cggcttcgcc gtaaacggtc caggtttcgg
    13201 cgagcggcgc cgggggggcc gctgaaaagt acgtcatctg aaaagcgagc agcgagccga
    13261 gcgccagtag gaatccgaag cgactcttct ttccccgcgg acggaccatg gtggttctcc
    13321 tttgctgata gcagaccctg ctcccaatgc cgccgcaaac cgggtcggca gcgcaccggc
    13381 tcatcgcgat gagaaagaag gatatgccgt atcctagttt tgttttcccc accaatcaac
    13441 accaatgttt taccggcagg ctttcgcacc gtggcagtct cgacagctcc ctcgcactcc
    13501 cgtcaaaggg cttcaactct tcccgctaga gcgcgaaagc tgcgggaagg cggatccatc
    13561 gccggtctta gcccaaaagc gtgaaggcag cacagttcac cggcaggaga tggtcccgct
    13621 tgacggtcag catggcctgg cgcggcgccc gggccggaag atgccgtctc gccgcaaagg
    13681 gagaggaaag gtccaaccgg aaggggctgc tgcgcaaact ggggatgcgg ccgcgaggaa
    13741 aatccggaca tctggcaatc cctcaacctc acaaaaagaa cattgttgag tggatgccaa
    13801 ggcagtggat gttttcgaga gtcgccgggg ttggaaggcc gcaccccggc aagggctcga
    13861 taaaggcaaa ctatccgaaa ggacaggacg cacagcttcc ggcttgggtc ctcaacggaa
    13921 aaggtcgcag ggctgccggg cgaatcctat gagagtggat cgcgacaaga gttcttgcgg
    13981 gtttcctccg cgttttgggt tctcgtttga agaaatcaag gctctgcatc acggactctt
    14041 gttgtaggac atccttgcgg ttgcggagag gctgccgccg tttcccgata tggtgtggaa
    14101 ggccgttccg ctcattcgaa gaatggctcc ggtcgaggga gtcgaggcgt cccggaaaga
    14161 cgtcacgggg cttgaccgca cgccggctcc ggctgtcgcc gtcgggagga gggcgtctca
    14221 gcaggtttcc gagtccttgc aggtcggtgt cccaaacccg gaactacacg ccgcgggacc
    14281 gcagggcccc cgccgggcgg gcgccgccgc cctggggttc acaggccttg gccgttggga
    14341 gatgcatgag aaggatcagg agggaatccc ttgtctttcc cgataagtct ttcatggccg
    14401 gcgtccgggc gggtatctta tggaaggtcg tcccccttgt atagtatcat ccccgtccgg
    14461 gcgcggtcga aatcggtcgt gagaacgccc accggccgac ctcggggatc catcagccgg
    14521 gggagccgaa ggaaacctat cgggaggaga aacgcaggca tggaatacga tgaaggaacg
    14581 atgggccgga tctttaccct ccggctggga gacggagacc ggttgcccga caccatcgag
    14641 gtgtttgccc aggccaaaag gattcgaaac gccctggtgt tctatctggg aggcgccgcc
    14701 gacggaagcc gcctggtggt gggccccgag gaaaaccggg gggacgccgt cgttcccatg
    14761 attcacaccc tccggggcgc ccaggaggtc ctggccgtgg gaacgctgtt tcccagcgaa
    14821 tccggggagc cggtcctcca tatgcacgcg gc
//
LOCUS       DAOOVC010000099        14517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2771_length_14517_cov_6.085120, whole genome shotgun sequence.
ACCESSION   DAOOVC010000099 DAOOVC010000000
VERSION     DAOOVC010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 14517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14517
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2771_length_14517_cov_6.085120"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="PLM79_12290"
     CDS             complement(<1..1223)
                     /locus_tag="PLM79_12290"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOV87136.1"
                     /translation="MKRFSLGPFFTRLASPGELLVLFATVCFLTFALGMPPSCSWAAS
                     SVNVPLDHWAYGALDKLDGFGLIHSDLQGTRPFSRKEFARLIQEAATEKARLGKDLPS
                     LPSYLLEKLQREFKRELSTLKAEEGRSSLSHIKPLQEFQARYVYTDGDPRHYRGYAMG
                     SKGMNATEGNPMVNNNDGLIYGQHHNLQLRFSTSADFKDWASAYLEPYLLVRQGDGRP
                     GDFDEVQADLLRGYGKLAPWNVEVLVGRDSLWWGQGRHGELLMSNNAFPLDMVKLSNP
                     EPVILPWYLGYLGPFKYTLFLSRLEDLLVMSPPPNREPYYTDVGFFGVRANFKPHPVF
                     EMGISITTIFGGDRQPSLSWKDIAGFFGVASGSNVKMNQLAELDFRFQLPFLRNIELY
                     GSYAGEDTGGLEYPE"
     gene            complement(1747..1998)
                     /locus_tag="PLM79_12295"
     CDS             complement(1747..1998)
                     /locus_tag="PLM79_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87137.1"
                     /translation="MKREDRKSKVRAERVDTKPGSIGHMTVDQIKERTIGENLNVTCP
                     ECGEIHLTLEDIEEAEKKKITKTAKYRKIRAEAVARTDR"
     gene            complement(2223..2672)
                     /locus_tag="PLM79_12300"
     CDS             complement(2223..2672)
                     /locus_tag="PLM79_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV87138.1"
                     /translation="MPFEPLILAFACDWCSYPAADLAGTVRMQYPTNIRIIRVTCSGM
                     VHPNLVVEAFHQGADGVMVMGCHPGECRYLDGNTKAEARMAIIEDVLSVLGVEKERFR
                     LVWLSSAEAERFVELVKEMTESVRRLGPSPLGFDEAPALGPGDPSGE"
     gene            complement(2724..5771)
                     /locus_tag="PLM79_12305"
     CDS             complement(2724..5771)
                     /locus_tag="PLM79_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87139.1"
                     /translation="MLLQEKIGAVMVVGGGIAGIQAALDLADSGYYVYLVERSGAIGS
                     TMAKLAKTFPTNDCSMCILSPKLVECSRHLNIELKTLTEVVKIAGEVTNFTVTLRQQP
                     RYVDMDRCIACAKCSRRCPRDVPDEFNERIGFRKAIYVRYPQAVPLKYQVDPEHCDRL
                     LGGDCRICEDACPAHALRFDDQVREYSVRVGSIIMAPGYRTFDPRGIATWGYGVFPNV
                     ITSIELERYLSASGPTGGRLLRPSDRKEVRKMAFLQCVGSRDYTPPSHGYCSSVCCMT
                     AIKEAMISMDRVQDLEVSVFYMDMRTHGKGFERYYERAKARGVRFHRCRVHSLEPLAD
                     HRLYFRYITDEGKQEKDEFDLVVLSVGMESPPEAIRLAELAGAELNHNRFVVTSSLSP
                     VTTSRPGIFVCGAFSGLRDIHQAVVEASAAAGAAAIPLTDVRYNQWRQREFPPERDVS
                     RGPPRIGLFLCECGTNISGIIDMEALERYGATLPHVVYTERNLFACSEDSQIALRRRI
                     EEEGLNRIVIAACSPHTHEALFRNTLKTCGLNENLIEMANIRNQASWVHALEPAAATN
                     KAKDLIRMAVAKVTLLQPLPCVSVNVNPLALVIGGGMAGMVAALGLADQGFPVHIVEK
                     TSELGGNARHLFKTWKNEDVPPFVNDLITRVKEHELITVHMRSRVVHAEGFVGNFRST
                     IQKNATHSVIDHGAAVIAVGGQAYKPDEYGYGQSQRVFTTLEFDKLHQVGDERVKYGS
                     NFAFIQCVGSRTPERPYCSRVCCTHAIQSAIELVEENPERNVFVLYRDIRSFGQREEL
                     YRRARELGVIFINYDIHEKPEVRVRENGLQLVVWDHVLHERVSLSADVLVLSTAIVPQ
                     PEVRELANLYKIPVDADGFLQEAHGKLRPVDCAAEGIFLAGLAHYPKPIEEAVAQAQA
                     AVSRAVTVLSKRRIDLESVRAMVDPERCDGCALCLDVCPYHAISMEDPRRLGGKQRIH
                     ISAAKCKGCGLCQATCPKDGVNVGGFTNRQLSAQVKAAIKKEI"
     gene            complement(5827..7269)
                     /locus_tag="PLM79_12310"
     CDS             complement(5827..7269)
                     /locus_tag="PLM79_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083764131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV87140.1"
                     /translation="MIVLRVSQLAERLGVHRNTIRNWIKSGKLPTRSVDGKRYLITEE
                     DFSRLCQQFGLDGSSMRLKYVPGGPVIEKPAVKPDENLRKIGTRSLRLRPDPQWADVC
                     VTCGSCASACPLSGVDGMDPRKAIRMAVLGLEDELIESQWPWKCTLCGKCEEACPMRV
                     EIVSLLQLVRRMRPRERIPGPLHKGVLMCIEKGNNLGIPKDDFVSLLREMGREMNEDG
                     YLGFSAPIDVRGANLMVTVNSKEPFAEPDDMKFWWKIFYAAGESWTIPSEYWEGVNWG
                     FLTGDDDAMRTIVGRIVQNMHRLECKTLLLPECGHAYYATLLGLHRWFKDDLQTFEVV
                     SVFDLLYRYLQEGRIRVDPSRHPQLTTYHDSCNYGRHSLKAFGKAYFEEGRAITRQCA
                     PNFVEMYPNRAGNYCCGGGGSAWAVPFKEERVFHGRFKARQIENTKAKLVIASCHNCR
                     DQIMKSLRKEYNLDIQVKYLWELVADSLVL"
     gene            complement(7266..7760)
                     /locus_tag="PLM79_12315"
     CDS             complement(7266..7760)
                     /locus_tag="PLM79_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOV87141.1"
                     /translation="MLSKVLHCSTLILGCGNPLFGDDGFGPAVIDHLLSRYTLPDPVA
                     AVDVGTGMGSFLVDLLLFPQKPRRIFILDAVSDSDRRPGEIFELPLSSLTGKDTTVIT
                     LHEFPSAQVLQELEQQAGVKVHILVVQAQSIPKEVSPGLSPPVLEAVSQASLWLYRQV
                     EEEP"
     gene            complement(8020..8499)
                     /locus_tag="PLM79_12320"
     CDS             complement(8020..8499)
                     /locus_tag="PLM79_12320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOV87142.1"
                     /translation="MVLILGYGNSLRRDDGAGLLLAEKLERLWMDAGVEVKCLLVHQL
                     TPDLAEEISRQGLDSVVFVDARVVAGPPDDLTIHLEPLEPSEMSLSPGHQSDPGSLLL
                     IARELFGRVPTAWLVTVPGVDFGLGESLSPVAQEALDHIPSCLNLPPGRRDAPFPPS"
     gene            complement(8543..9997)
                     /locus_tag="PLM79_12325"
     CDS             complement(8543..9997)
                     /locus_tag="PLM79_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOV87143.1"
                     /translation="MAQTLHIEPVTRIEGHGKISIHLDDAGQVTDARFHVTEFRGFEK
                     FCEGRTYPEMPGITMRICGICPVSHMLASAKACDNIQSAKVPPTAIKLRRLINHAQYV
                     QSHALSFFHLSAPDLILGMESDPATRNVVGIIQANPELGRRAIRLRQFGQEIIRGVTG
                     KSVHPSWATPGGVRDPLKTEQIEYIKKNLPEAFETVEIALNWLKDHSDRFEREKATYG
                     DFPSLYIGLVTPEGGLEHYDGLLRVMDADGKILEPGLPLDRYREIIAEAVEPWSYLKF
                     PYYKPYGYARNGDGVRPGMYRVGPLARLNLCDFAGTPLADRELRQFRRLANSPGKPVS
                     SSFHFHYARLIELTFALERIAELVADPDILDTHIRSQAKVNELRGVGIMEAPRGTLIH
                     DYEVDETGILKKVSLLVATGNNNLAMNKTVEQIAKEYLSGATITEGLLNRVEHGIRLY
                     DPCLSCSTHAVGKMPLHVQVVDSKGEILDERYRD"
     gene            complement(10083..10634)
                     /locus_tag="PLM79_12330"
     CDS             complement(10083..10634)
                     /locus_tag="PLM79_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOV87144.1"
                     /translation="MTTQNSKVRLATTWFGGCAGCHMSFLDLDERLIDVLGLVDIVFG
                     PVVDIKKFPDNVDVTLIEGAVANSDHVKEAREIRRKSKIVISFGDCAVTGNVPAMRNA
                     LKIDDVLTAVYAEGPGKSPPSGELLDIMPVLLPKVLPVHQVIPVDLYLPGCPPNPDRI
                     FAAVAALLKGEPVTLPEEMRSFG"
     gene            complement(10647..11372)
                     /gene="hoxU"
                     /locus_tag="PLM79_12335"
     CDS             complement(10647..11372)
                     /gene="hoxU"
                     /locus_tag="PLM79_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxU"
                     /protein_id="HOV87145.1"
                     /translation="MSVVTLTIDEKLVSAQAGKTVLDAAREAGIDIPTLCHLEGLTPR
                     GGCRLCLVEMEGNPRLLASCVTQVQEGMIIRTHTERIVKYRKMMIELLLAERNHVCAV
                     CVMNGHCELQAMAAKLGVDHVRYDYLNPALAMDASHERNVRDPNRCILCARCVRVCDE
                     VEGAHCLDMMGRGIHCNVITGMNQPWGKAKSCTACGKCVQVCPTGALFKQGATVAEME
                     KQHDFLIWILDGRDKKIWHWSRQ"
     gene            complement(11432..13093)
                     /locus_tag="PLM79_12340"
     CDS             complement(11432..13093)
                     /locus_tag="PLM79_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOV87146.1"
                     /translation="MNREELKVTAQVELDHQARFRCRILCCMSTPCISSGAESVLAAL
                     TRSIEAGQMQTEVESVATGCMGPCSRGPLVTVKTEGKPDVIYENVTPDMVDSILARHV
                     QGKTAPELDDKVLPNDIPFFAKQEKIVLSASGLVDPERLESYIARDGYSALGHALREM
                     TPEEVCEAIAGSGLRGRGGAGYPTGLKWHMVRKAPGERKYVVANGDEGDPGAYMDRTL
                     MESDPHRVLEGMAIAGYAVGAEQGYIYVRGEYPLAAKRLEKAIRQAERKGLLGNRIMD
                     SPFNFRIDLRMGAGAFVCGEETALMQSIMGRRGQPVPRPPYPAQKGLWGGPTLINNVE
                     TFGNIAMIIAKGASWYAGFGTEKSKGSKTFALAGKVVNTGLIEVPIGITLREIVYEIG
                     GGIPGGRAFKAAQTGGPSGGCIPAEFLDVPIDYESLAKLGSIMGSGGLIVMDETSCMP
                     DVAKFFMEFCMDESCGKCNPCRGGTVQMHRILEHITDGSATMDDLTKLEDLCMMVKET
                     SLCGLGQTSPNPIYSTLRYFRDEYEAHIRDHHCPAGVCQFGAKAQ"
     gene            complement(13256..13786)
                     /gene="hoxE"
                     /locus_tag="PLM79_12345"
     CDS             complement(13256..13786)
                     /gene="hoxE"
                     /locus_tag="PLM79_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bidirectional hydrogenase complex protein HoxE"
                     /protein_id="HOV87147.1"
                     /translation="MAETSPKKKDAAEHPMGDPRFKGIDRVLKRQQYQQDALIEVLTA
                     AQEAFGYLTEDAMAYVARQLRLPFSWVYGVATFYHFFSLKPQGEHSCIVCLGTACYVG
                     KAADIVEALEGGFAVKAGQTTEDGKLTLTTTRCLGCCGLAPVVVLDNAVLGKETPETT
                     VEKVRSVVAGGNGGGA"
     gene            complement(14184..>14517)
                     /locus_tag="PLM79_12350"
     CDS             complement(14184..>14517)
                     /locus_tag="PLM79_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87148.1"
                     /translation="VVQQNAADAEESASSSEEMNAQAEQLRAFVQDLAVIVEGEVDGS
                     RKKKPRTPDGAGDRPGGLMSIVRAFLRPEAKASLPGDTTRGEEQTKNPMLLLRQEPSP
                     RDRETRDF"
BASE COUNT         2737 a         4343 c         4476 g         2961 t
ORIGIN      
        1 tcttcgggat actcgaggcc gccggtgtct tctcccgcgt aggacccgta gagctcgatg
       61 tttctcaaaa acgggagctg gaaccggaag tcgagctccg ccagctggtt catcttgacg
      121 ttggagcccg atgcgacacc gaaaaatccc gcgatgtcct tccaggacag ggaaggctgt
      181 cgatctcctc cgaaaatggt ggtgatggaa atgcccatct cgaagacggg gtgaggtttg
      241 aaattggcgc gaacgccgaa aaaccccacg tcggtgtagt agggttcccg gttgggggga
      301 gggctcatga cgagcaggtc ctcgaggcgg gagaggaaaa gcgtgtattt gaacggcccc
      361 aggtagccca ggtaccaggg aaggatgacc ggctccggat tggacagctt caccatgtcc
      421 agggggaagg cgttgttgct catgagcagt tccccgtgtc tgccctggcc ccaccacagg
      481 gagtctctcc cgacgagcac ttccacgttc cagggtgcca gctttccgta gcctcgaagc
      541 aagtccgcct ggacctcgtc gaaatccccc ggtcggccgt ccccctggcg gacaaggagg
      601 tagggctcga ggtaggcgga cgcccaatcc ttgaagtccg cactggtgga gaaccggagc
      661 tgcagattgt gatgctgacc gtagatgaga ccgtcgttgt tgttcaccat gggattgcct
      721 tcggtggcgt tcattccctt ggaccccatg gcatagcccc ggtaatgacg ggggtcgccg
      781 tccgtgtaca cataacgggc ctgaaattcc tgcaggggtt tgatgtggga gagggaagac
      841 cttccttctt ccgctttcag cgtggacagt tccctcttga actcccgctg gagcttttcc
      901 aggagatagg agggcaggga ggggagatct ttgccgaggc gcgctttctc cgtcgcggct
      961 tcctggatga ggcgggcaaa ttctttccgg gaaaagggtc gcgtgccttg aaggtcggag
     1021 tggatcaggc cgaatccgtc cagcttatcc agggccccgt aggcccagtg gtccaggggg
     1081 acgttgaccg aggacgccgc ccaggaacag gaagggggca ttcccagggc aaaggttagg
     1141 aaacagacgg ttgcgaaaag gacaagaagc tctcccgggg atgcaaggcg agtgaagaac
     1201 ggtccgagac tgaagcgttt catgcttttc ctttttcata tcctgcggat ttcaacggct
     1261 cctcccggct tgcgccgggc cccccgccgg gacccctcga gatcgcgccc cctgtcccgg
     1321 agaaatgaaa acgaaaaaaa aggcgctacg gctcggtcgg catgcttttt accgctcatt
     1381 gtcgaagatg gtcccggagg gacatgcacg gcggcgatca ttccggagcg atagcctctg
     1441 aagggcagac cggcgcaaaa ccgttcccgt tccggaggtt gaagacggca ttgcagacat
     1501 gggatcttgg gccgggaagg attcccagca agagtgaata ctcaaggaac cccaaaagat
     1561 caagtgcaat ttctcccccc caaacggcgc aacggagcga tcccccgcag ccttggaggg
     1621 gcttgcccac agggtccccg aggaccattc ccgcaacggt tcgcgcccca gtcggggggc
     1681 aacggaagtc cgcaggaacc cattgggacc tggggagaag agggggcccg gaagggaggg
     1741 aaagggtcag cgatccgtgc gggctacggc ctcagctcgg atcttccggt atttagccgt
     1801 cttggtgatt ttcttctttt ccgcttcttc gatatcttcc aaggtgagat gaatctcgcc
     1861 gcattcgggg caggtcacgt tgaggttctc gccaatggtg cgctctttga tctggtccac
     1921 ggtcatgtgt ccaatggacc cgggtttcgt gtcaactcgt tccgctcgga ccttcgactt
     1981 tctgtcttcc cgcttcatgc caggctcctc gtcattggct ggtttcttgg accgaactgg
     2041 gtgccacgtc gggattcgga aatgcccgga ggacgtgtgg ccgaatgaac cgcaaggctt
     2101 tctcctatat aaagccggaa agcaaccgga tgcaaggatc tttccatcca cggccgcgaa
     2161 aagttgtgca aaatgattgt gcattgtgcc tacggcctac caaagggggg aagaaaggag
     2221 aatcactcgc ccgaaggatc cccgggaccc agggcgggtg cttcgtcgaa tcccaggggg
     2281 gacggtccga gtctcctcac ggactcggtc atttccttga cgagttccac gaaccgttcg
     2341 gcctcggccg aggacagcca aaccagccgg aaccgctcct tctcgacccc caaaaccgac
     2401 agaacgtcct cgattatggc catcctggcc tcggccttgg tgttgccgtc caggtaacgg
     2461 cattcgccgg ggtggcatcc catgaccatc accccgtcgg cgccctgatg gaacgcttcc
     2521 accaccaggt tgggatgaac catcccgctg catgtaaccc ggatgattcg gatattggtc
     2581 gggtattgca tccgaaccgt cccggccagg tctgccgcgg gataggaaca ccagtcacag
     2641 gcgaatgcga gaatgagggg ttcaaagggc atcgggtttt ccctccaaag caacagactt
     2701 tagagactgc gcggaagcga ggatcagatt tccttcttga tggccgcctt gacttgggcc
     2761 gagagctgtc ggttggtgaa cccgcctacg ttgaccccgt ctttgggaca ggtggcctga
     2821 caaagcccgc aacccttgca cttggcggcg ctgatatgaa tacgctgttt gcctcccagt
     2881 ctgcggggat cttccatgct gatggcgtgg tacgggcaca catccaggca cagggcgcag
     2941 ccgtcgcagc gctcggggtc caccatggct cggacggatt ccaggtcgat tcggcgcttg
     3001 gacaagacgg tcaccgcccg ggacacggcg gcctgggcct gggccaccgc ttcctcgatg
     3061 ggcttgggat aatgggccag gcccgccagg aagatccctt cggcggcgca gtccacgggg
     3121 cgaagtttgc cgtgggcttc ctgcaaaaag ccgtccgcat ccacgggaat cttgtagaga
     3181 ttggccagct cgcgcacctc gggctggggc acaatggccg tggacaagac caggacgtcg
     3241 gcgctcaagc tgacccgttc gtggagaacg tgatcccaga ccaccagctg gagcccgttt
     3301 tcccgcacac ggacctccgg tttttcatgg atgtcgtaat tgatgaagat cactcccagt
     3361 tcccgcgccc tccggtacaa ctcctccctc tggccgaagg agcgaatgtc ccggtagagt
     3421 acgaagacgt ttcgttcggg attttcttcc acgagctcga tggcggactg gatggcgtgg
     3481 gtgcagcaga ccctcgagca atagggcctc tcgggggtcc tcgatcccac gcactgaatg
     3541 aaggcaaaat tcgacccgta tttgacccgc tcgtctccca cctggtgcag cttgtcgaac
     3601 tcgagggtgg tgaagacccg ctgagactgg ccgtatccgt attcatccgg cttgtaggcc
     3661 tggccgccca cggcaatgac ggcggccccg tggtcgatga cggagtgggt ggcgttcttc
     3721 tggatggtgg aacgaaagtt gcccacaaat ccctcggcat gcaccacccg gcttctcatg
     3781 tgcacggtga tcaattcgtg ttccttgacc cgggtgatga gatcgttcac gaagggagga
     3841 acgtcctcgt tcttccaggt cttgaagagg tgcctggcgt tgccgcccaa ttccgaggtt
     3901 ttctccacga tgtgaacggg aaacccttgg tcggccagtc ccagggccgc caccatcccg
     3961 gccatccctc cgccgatcac cagggccagg ggattcacgt ttacggaaac acacgggagg
     4021 ggctgaagga gggtcacttt tgcgacggcc atgcggatca ggtccttggc cttgttcgtg
     4081 gcggctgcgg gctccagggc atggacccag gaggcctgat tgcggatgtt ggccatctcg
     4141 atcaagtttt cgttcaaacc gcaggtcttc agggtattgc ggaacagggc ctcgtgggtg
     4201 tgaggggaac aggcggcgat cacgatgcga ttgagcccct cctcctcgat tctgcggcgc
     4261 aacgcgatct gagagtcctc ggaacaagcg aacaggttcc tctccgtgta gaccacatga
     4321 gggagcgttg ccccgtacct ttccagggcc tccatgtcga tgatccccga gatgttggtc
     4381 ccgcattcgc acaggaacag accgatccgg ggcggtcccc gggacacgtc ccgttcggga
     4441 gggaactccc gctggcgcca ttggttgtac cggacgtccg tgaggggaat ggcggcggct
     4501 cccgccgcag ccgaagcttc caccaccgcc tggtgaatgt ccctcagacc ggaaaacgcg
     4561 ccgcatacga agatcccggg cctgctggtg gtgacggggg acagggagga ggtgaccacg
     4621 aagcggttgt ggttgagctc cgctcccgcc agctccgcca gccgaatcgc ctcgggaggg
     4681 gattccatgc ccacggaaag gaccaccagg tcgaattcgt ccttctcctg ttttccttcg
     4741 tcggtgatgt agcgaaaata gaggcgatga tccgccaggg gttccaggga atggacccgg
     4801 cacctgtgga acctgactcc ccgagccttg gccctctcgt agtaacgttc gaagcccttc
     4861 ccatgggttc gcatgtccat gtagaagacg gaaacctcga ggtcctggac ccggtccatg
     4921 gatatcatgg cttccttgat ggcggtcatg cagcagacgg aggagcagta gccgtgggaa
     4981 gggggagtgt agtcccggct gcccacgcac tggaggaagg ccattttccg gacttccttc
     5041 cggtccgaag gcctgaggag ccgacctccg gtggggcccg aggcggaaag gtagcgttcc
     5101 agctcgatgg aggtgatcac gttgggaaag accccgtatc cccacgtggc aatgccccgg
     5161 gggtcgaagg tgcgatagcc gggggccatg atgatggagc cgacccgaac ggagtattcc
     5221 cgcacctggt cgtcgaaacg caaggcgtgg gcgggacacg catcctcgca gattcggcag
     5281 tctcccccca gcagccggtc acagtgttcg gggtccacct ggtatttgag ggggacggcc
     5341 tgaggatacc gaacatagat ggccttgcgg aacccgatgc gctcgttgaa ctcatccggt
     5401 acgtcccggg gacaccttcg ggaacacttg gcgcaggcga tgcaccggtc catgtccacg
     5461 tatcgaggct gctgacggag ggtgacggtg aaattggtca cttcccctgc gatcttgacc
     5521 acctccgtca gggtcttcaa ttcgatgttc aggtgccggc tgcattccac cagcttagga
     5581 gaaaggatgc acatggagca gtcgttggtg ggaaacgttt tggccagctt ggccatggtg
     5641 ctcccaatgg cccccgagcg ttccaccagg tagacgtagt acccgctgtc ggccagatcc
     5701 agggccgcct ggatcccggc gatgcctccc cccaccacca tcacggcacc gattttctcc
     5761 tgcagcagca tgtcgtgtgc tcccgtaccc taattcttcc cgttgccgcg gatgttcgcc
     5821 ggtaggctag agcaccaggg aatccgccac cagctcccag agatacttca cctggatgtc
     5881 cagattgtat tcctttcgaa ggctcttcat gatctggtcc cggcagttgt gacaggaggc
     5941 gatcaccagc ttggccttgg tgttctcaat ctgccgggcc ttgaaccgcc cgtggaaaac
     6001 ccgctcctcc ttgaaaggga ccgcccacgc acttccgccg ccgccgcagc agtaattgcc
     6061 cgcgcggttg ggatacattt ccacgaagtt gggagcgcac tggcgggtga tggcacgccc
     6121 ctcctcgaag taggcctttc cgaaggcctt gagggaatgg cgcccgtagt tgcaggagtc
     6181 gtggtaggtg gtgagctggg ggtgccggga ggggtccacg cgaatccgac cctcctgaag
     6241 gtaccggtac agcaggtcga aaaccgaaac cacctcgaag gtttgcaagt cgtccttgaa
     6301 ccatcgatgc agccccagga gggtcgcata ataggcatgg ccgcattccg gaagcagcag
     6361 ggtcttgcac tcgagccggt gcatgttttg gacgattctc cccacaatgg tcctcatggc
     6421 gtcgtcgtcc cccgtcagaa agccccagtt gacgccctcc caatactccg aagggatggt
     6481 ccaggattcc ccggcggcgt agaagatctt ccaccagaat ttcatgtcgt cgggttcggc
     6541 gaagggctcc ttggagttga ccgtcaccat gaggtttgct ccccgaacat caatgggggc
     6601 ggagaagccc aggtaaccgt cttcgttcat ttcccgcccc atttcccgga gcagggatac
     6661 gaaatcgtcc ttggggatcc ccaggttgtt ccccttctcg atgcacatga ggacgccctt
     6721 gtgcaggggg cccgggattc gttcccgggg tcgcatcctt cggaccagct gcagcaggga
     6781 gacgatttcc acccgcatgg gacaggcctc ttcgcacttg ccgcacaagg tgcacttcca
     6841 cggccactgg gattcaatga gttcgtcctc gaggcccagg accgccatgc gaatggcctt
     6901 ccgggggtcc atgccgtcta cgcccgaaag gggacacgcg ctggcacagc tcccgcaagt
     6961 gacgcatacg tccgcccact gggggtcggg gcgcagtcga agagagcggg tgccgatctt
     7021 tctcaagttt tcgtcgggtt tgacggcggg cttttcgatc accggccccc cgggaacgta
     7081 cttcaacctc atggaagagc cgtccagacc gaactgctgg cacaatcggg agaaatcctc
     7141 ttccgtgatg aggtatcgct tgccgtccac ggaacgagtg ggaagcttgc cgctcttgat
     7201 ccagttgcgg atggtgttgc ggtggactcc gagcctctcg gccaactggc tcactctcag
     7261 gacaatcatg gttcttcctc gacttgacgg tacaaccaca gggacgcttg ggaaacggcc
     7321 tccaggaccg ggggggaaag gccgggggaa acctccttgg ggatggattg ggcctgtacc
     7381 accaggatgt gaaccttcac gcccgcctgc tgttccaact cctgcaggac ctgagcggaa
     7441 ggaaactcat ggagggtgat gaccgtggtg tccttgccgg tgaggctcga gaggggaagc
     7501 tcgaaaatct ccccgggccg ccggtccgag tcggaaacgg catccaggat gaagatcctt
     7561 cgaggctttt gagggaagag caggaggtcg accaggaagc ttcccatgcc cgtgcccacg
     7621 tccacggcgg ccacggggtc cggcagggtg taacggctca gcaaatggtc gatgacggcg
     7681 ggtccgaatc cgtcgtcccc gaaaagggga ttgccgcaac cgagaatgag ggtggagcag
     7741 tgcagcacct tgctgagcat gacgttcatc cgcgctgaag atggctgagg tcccggattc
     7801 actcccccat cttgtgcact ttgtgcacga gaaacggtgg ttgtatacta cgtttgcaaa
     7861 gtgaaatttg tcaacaggag tttcgcataa cgaaaaaaga ttcacaggga atagggcatt
     7921 cttctcccca aggctgcagg ctttgccggg acggccggag aaggtggtcc caacggctcg
     7981 gagacggttt ccgggggata gagaagaagg gccttggaat cacgagggag gaaacggtgc
     8041 atcccggcgc ccggggggaa ggttcaggca ggaagggatg tggtccaggg cctcttgggc
     8101 caccgggctc agggattctc ccaggccgaa atccaccccg ggaacggtga cgagccatgc
     8161 ggtcggcact cgaccgaaaa gctcccgggc aatcagcaga agggagcccg ggtcggactg
     8221 gtggcccggg gacagggaca tttcggaggg ttccaggggt tcgaggtgga ttgtgaggtc
     8281 gtccggggga ccggcgacca cccgggcgtc cacgaagacc accgaatcca ggccctgccg
     8341 ggaaatttcc tcggctaggt ccggggtgag ctggtgcacc aggaggcact tcacctcgac
     8401 cccggcatcc atccagagtc tctcgagctt ctcggcaagc aacaagcctg cgccgtcgtc
     8461 acggcgcagg ctgtttccgt atccgaggat caaaaccact gtttcgttcc cggtgaaggt
     8521 gcgggaaagg ctcgtcgtgt ttttagtccc gataacgctc gtcgaggatc tctcccttgg
     8581 aatccaccac ttgcacatga aggggcattt tgcccacggc atgggtcgaa cagctcagac
     8641 aggggtcgta gagccgaatg ccgtgctcca cccggttcag gaggccttcg gtgatggttg
     8701 caccgctcag gtactccttg gcgatctgct ccacggtctt gttcatggcc aggttgttgt
     8761 tccccgttgc caccaggagg ctgactttct tcagaatccc cgtctcatcc acttcgtagt
     8821 cgtggatgag ggtgccccgg ggtgcctcca tgatccccac tccccgaagt tcgttcactt
     8881 tggcctggct ccgaatgtga gtatccagga tgtcgggatc ggccaccagc tcggcgatac
     8941 gttcgagggc gaaagtgagc tcgatgagcc gggcatagtg gaagtggaag ctgctgctga
     9001 ccggtttgcc cggtgaattg gccagcctcc ggaactgcct caattctcgg tccgccaggg
     9061 gggtccccgc aaaatcacag agattgagac gcgccagggg tcctacccgg tacattccgg
     9121 gccggacgcc gtcaccgttt cgggcgtagc cgtagggttt gtagtagggg aacttgaggt
     9181 agctccaggg ttccaccgct tcggcgatga tctcccggta ccgatccagg ggcagtcccg
     9241 gttccaggat cttcccgtcg gcatccatga cccgcagaag gccgtcatag tgttcgaggc
     9301 cgccttccgg ggtcaccagc ccgatgtaga ggctggggaa gtccccgtag gtcgcttttt
     9361 cccgctcgaa ccgatcggag tggtccttga gccagttcaa ggcgatttcc acggtctcga
     9421 aggcctcggg caagttcttc ttgatgtact caatctgctc ggtcttgaga gggtcccgga
     9481 cccctccggg ggtagcccag gagggatgga cgctctttcc ggtcaccccc cggatgatct
     9541 cctggccgaa ctgacgcagg cggatggccc gacgccccag ttcggggttg gcttggatga
     9601 ttcccaccac gttccgggtg gccgggtcgc tttccatccc caggatgagg tccggagcac
     9661 tgaggtgaaa aaagctcaag gcgtggctct ggacgtattg ggcatggttg atgaggcggc
     9721 gaagcttgat ggccgtcggc gggaccttgg cgctttggat gttgtcgcag gcttttgccg
     9781 aagccagcat gtgactcacc gggcagatgc cgcagattcg catggtgatg ccgggcattt
     9841 ccggataggt ccttccttcg cagaatttct cgaagccccg gaattccgtc acgtgaaacc
     9901 tggcatccgt cacctggccg gcgtcgtcca ggtgaatgga aatcttgccg tggccttcaa
     9961 tccgagtgac aggttctata tggagtgtct gtgccatagt cgtccttctt taccgacaga
    10021 ttttctggtt cgctgggata cggacgaggg tcgggtccgt cgtccggccg ctctttacgg
    10081 aactacccga agctgcgcat ctcctcgggc agggtcaccg gttccccctt gagcagggcc
    10141 gcgacggcgg caaaaatgcg gtccggatta gggggacatc ccggcaagta aaggtccacg
    10201 gggattacct ggtgaaccgg gaggaccttg ggcaacagaa ccggcatgat gtccagaagc
    10261 tccccgctgg ggggggactt gccggggcct tcggcataga cggccgtgag cacgtcgtcg
    10321 atcttcaggg cgttgcgcat ggcggggacg tttcccgtca ctgcgcagtc gccgaaggag
    10381 atgacgatct tgctcttgcg gcgaatctcc ctggcctcct tcacgtggtc cgaattggcg
    10441 acggctccct cgatgagggt cacgtccacg ttgtccggga acttcttgat gtccaccacg
    10501 gggccgaaca caatgtccac cagccccagg acatcgatga gccgttcgtc caagtccaga
    10561 aagctcatat ggcagccggc gcaaccgcca aaccacgtgg tggccaaccg tactttactg
    10621 ttttgtgtgg tcatgtcggg cttctcctat tgtctggacc aatgccatat ctttttgtct
    10681 cgaccatcca gaatccagat caggaagtca tgctgcttct ccatttcggc caccgtggct
    10741 ccctgcttga agagcgcccc cgtgggacat acctggacgc acttgccgca tgcggtgcag
    10801 ctcttggcct tcccccacgg ctggttcatt ccggtgatga cgttgcaatg aattccgcgc
    10861 cccatcatat cgaggcagtg ggccccttcc acctcgtcgc agacccggac gcaccgtgca
    10921 cacaggatgc accggttggg gtcccggaca ttacgctcgt ggctggcatc cattgccagg
    10981 gcgggattca ggtagtcgta gcgcacgtgg tccacaccga gctttgccgc catggcttgg
    11041 agttcgcaat ggccgttcat gacgcagacg gcgcatacat gattgcgctc ggccaggagg
    11101 agctcgatca tcatcttgcg gtacttgacg atccgctcgg tgtgagtccg gatgatcatg
    11161 ccctcctgga cctgggtaac gcacgaggcc aggagcctgg ggtttccctc catctcgacc
    11221 aggcaaaggc ggcagccgcc ccgaggggtc agcccttcca ggtggcagag ggtgggaata
    11281 tcgatcccgg cttcccgggc agcgtccaga acggttttgc ccgcttgggc gctgaccagt
    11341 ttttcatcga tggtaagggt gacaaccgac attatgatac ctccgttggt gatgggaccg
    11401 ttcgaggttc cgaaaacact ccttcgcgag cctattgggc cttggcgccg aactggcaca
    11461 ctccggccgg gcagtggtga tccctgatat gggcctcgta ctcatcccgg aagtaccgca
    11521 gggtgctgta aatcgggttg ggggaggtct gacccaagcc gcacaggctg gtttccttca
    11581 ccatcatgca caggtcctcg agcttggtca ggtcgtccat ggtggccgag ccgtcggtga
    11641 tgtgctcgag aattcggtgc atctggaccg tgcctccgcg gcagggattg catttgccgc
    11701 agctctcatc catgcagaat tccatgaaaa acttggctac gtccggcatg cagctcgtct
    11761 cgtccatgac gatgagtccc ccggagccca tgatggagcc cagttttgcc aggctctcat
    11821 agtcgatggg gacatccagg aactcggcgg gaatgcagcc cccgctgggc cctcccgtct
    11881 gggccgcctt gaaggcacgc ccgccgggaa tcccgccgcc gatctcgtag acgatttccc
    11941 ggagggtgat tccgatgggg acctcgatca agccggtgtt caccaccttc ccggccaggg
    12001 cgaaggtttt ggagcccttg ctcttctcgg tgccgaaacc ggcataccag gaggcccctt
    12061 tggcgatgat catggcaatg ttgccgaagg tctcgacgtt gttgatcaga gtggggccgc
    12121 cccaaaggcc tttctgggcg gggtaggggg gccgcggtac gggctgtccc cggcgtccca
    12181 tgatggactg catcaaggcc gtctcttcgc cgcagacgaa ggcccccgcc cccatgcgca
    12241 ggtcgatgcg gaagttgaaa ggactgtcca tgatccggtt tcccaggagg cccttgcgct
    12301 cggcctgccg gatggccttc tccaggcgtt tggcagccag gggatactct ccgcgaacat
    12361 aaatgtaccc ctgttcggct cccacggcgt acccggcaat ggccatgccc tcgaggaccc
    12421 gatggggatc cgactccatc agggtgcggt ccatgtaggc gcccgggtct ccttcgtctc
    12481 cgttggccac cacgtatttt cgttcccccg gcgccttgcg taccatgtgc cacttcaacc
    12541 cggtgggata cccggcgcct ccgcgcccac gcagcccgct cccggcaatg gcctcgcaga
    12601 cctcctccgg ggtcatctcc cgcagggcat gacccagggc cgaataaccg tccctggcga
    12661 tatagctctc cagacgttcc ggatccacca acccgctggc ggacagaaca atcttttcct
    12721 gtttggcaaa aaacggaatg tcgttgggca gaaccttatc gtccagctcg ggagcggtct
    12781 ttccctgaac gtgccgggcc aggatggaat ccaccatgtc cggcgtcacg ttctcgtaga
    12841 tgacatccgg ttttccttcc gtcttgactg tgaccagagg tccccggctg cagggtccca
    12901 tgcatcccgt ggccaccgat tccacctccg tctgcatctg gccggcctcg atggaacgtg
    12961 tcaaggccgc caggaccgac tcggcgccgg aggaaatgca tggagtgctc atgcagcaga
    13021 gaattcgaca gcgaaatctc gcttgatggt ccagttccac ctgggccgtt accttgagtt
    13081 cttctcggtt cataacgctg tattctcctc agattctgac gttgtccagt cgattcgccc
    13141 gtgaaccttg cgcaggcctg ctctcgtcct cgtgtcgggg agtgccccgg agcttgcgta
    13201 agagcccgac gcggttgcct cctggaacgg gttcccctgc aatgggcaac caagatcagg
    13261 ccccaccgcc gttgcccccc gccaccacgg aacgcacctt ttccaccgtg gtctccggcg
    13321 tctccttgcc cagaaccgca ttgtccagaa cgaccaccgg cgccaatccg cagcagccta
    13381 ggcaccgagt ggtggtgagg gtgagcttgc cgtcctcggt ggtctggccc gccttcaccg
    13441 caaaaccgcc ctccagggcc tccacaatgt ccgcagcctt gccgacgtaa caggcggtac
    13501 ccaggcagac gatgcaggaa tgctccccct ggggcttgag ggaaaagaaa tggtagaagg
    13561 tggccacgcc gtatacccag ctgaagggaa gtctcagttg ccgtgccacg taggccatgg
    13621 cgtcctcggt gagatagccg aaggcctcct gggccgccgt gaggacctcg atgagcgcat
    13681 cctgttgata ctgctgacgt ttcaacaccc gatcaattcc cttgaatctc gggtccccca
    13741 tgggatgctc cgctgcatcc tttttctttg gactcgtctc ggccatagca gtttcctccg
    13801 tggtgccgat tcccgtgttc gttttcgcct ttttgttagg tatctcaact agctagagaa
    13861 atacccactt cgcccgcaca tgcagaccgc ctcgtttttg gttgctcaaa aaggaaggaa
    13921 ctccataaac ccggtttgca agaaacaacg cttgaaatcc acttaacagg tatgaacgac
    13981 gacttttacc cggacatgac cataatgaag gaaaaagacc aagtcaacaa aattttaact
    14041 gatataaaat attgttcaat attttcaaac caagacgggg agcggccggg cgggattcga
    14101 gaagggatgg ggcgaaagag ggagagggcg gggaaagatc cgccctcccg gttttcgacg
    14161 ggcactggtc gtgcgggtct ttgctagaag tctctcgtct cccggtcgcg aggagacggt
    14221 tcctggcgga ggaggagcat tgggttcttg gtttgttcct cgccgcgggt cgtatcgccg
    14281 gggagggaag ctttggcctc ggggcgcaag aaggctcgga cgatggacat gagtccaccc
    14341 ggccgatcgc ctgcgccatc cggtgtcctt ggcttcttct tccggctccc gtcaacctct
    14401 ccttccacaa tgacggccag atcttgaacg aaggctcgca actgttccgc ctgggcgttc
    14461 atctcctccg aggacgaagc ggattcctcg gcatcggcgg cattttgctg cacgacc
//
LOCUS       DAOOVC010000100        14313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2822_length_14313_cov_6.655983, whole genome shotgun sequence.
ACCESSION   DAOOVC010000100 DAOOVC010000000
VERSION     DAOOVC010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14313
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_14313_cov_6.655983"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLM79_12355"
     CDS             <1..1131
                     /locus_tag="PLM79_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOV87149.1"
                     /translation="LFMFLAIAFLEDTGYLARVAYMLDRVFRTFGLHGNSVMAYVISG
                     GIAGGCAVPGVMAARTLRSSRERLATLLTAPFMNCGAKLPVLAMLIAAFFPTRHAQVM
                     FLLTVLSWILALLLAKLFRTTILRGPSTPFLLELPPYRLPTFRGLLIHTWERTRQYIQ
                     KAGTIILAMSVLFWALMAFPGLPDSQKRRYEEQRSEILARFPPDLRIPLERNSIQPKD
                     LPSELVPWYEQLKAADRAEAQAALQHSWAGRMGIALESLSRWTGFDWRTNVALVAGFA
                     AKELIISTFGTAYSMGVVTQEEGGALSRQLASDPGWNPLRAFALILFIMLYAPCFATI
                     TCIIRESGSWKWGLFSAAFNTGAAFLISLLVYQGGRWLGIGT"
     gene            1147..1353
                     /locus_tag="PLM79_12360"
     CDS             1147..1353
                     /locus_tag="PLM79_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOV87150.1"
                     /translation="MWQMVLVLLVVAAVSVYIVRHFVRITRSETPTCGHCAGCPCTPS
                     STVPRECNPEQGNPCACENPPQEG"
     gene            1396..2490
                     /gene="aroB"
                     /locus_tag="PLM79_12365"
     CDS             1396..2490
                     /gene="aroB"
                     /locus_tag="PLM79_12365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOV87151.1"
                     /translation="MELTLELPGSPPRSSRVRIGEDILENLPESLSSRWEGSRIFWIW
                     DRAVHALWHPRLREMGWRPSSEDEWILFPASESNKRLSQLEELARRLVRAGADRRSVL
                     VAVGGGVTGDVVGFLASVYMRGIPHCQVPTTLLAQVDSSIGGKTGVDLPEGKNLLGTF
                     HQAHSIWMDIRFLETLPPEEFRQGMAEVIKTAMIGDPSLWEYLESHSEDVRSRRSESL
                     LRVVSSCCRLKARVVEADERESGYRMVLNLGHTVGHALEKTTGYAFRHGDAVAVGMAA
                     AARISLNRGELSMKNLQRLESLCRFWGLPTTLPGGSPPDEILAALSADKKRFLGVNHF
                     VLPRAMGEVTVRSDIALEEVRDVLRAMSSS"
     gene            2745..3836
                     /locus_tag="PLM79_12370"
     CDS             2745..3836
                     /locus_tag="PLM79_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87152.1"
                     /translation="MAEHSPHHPPPRSEALLWDILESVTDAIVTIDEDHKVVLCNRAA
                     EKMFGYLPEEMIGQDVSPLIPSSHRVLHKGYVERYLATGVPRVIGMLRECHAQRKNGE
                     SFPVEISYSVSRSGERTYFTAVIRDISQRKQMERDIHFMERLVDIGKAVSQITHEIRK
                     PLMLIGGFAHQLRSCDGLQADEKSRRKLDIILKEVQRLESLLNGIRLATRPGTSGRKQ
                     LLSVRKLLEETFELFESTLRETRIRFTAELDPDPLMVEGDSDQLKQVFLNMMQNAVEA
                     MNGVGTLTVGTRIGDGTIQVLIRDSGPGIPVEIRERIFDPFFTTKTEGTGLGLAISRN
                     IVQDHGGAISVESSSRGTTFLLQLPPGFP"
     gene            complement(3900..4583)
                     /locus_tag="PLM79_12375"
     CDS             complement(3900..4583)
                     /locus_tag="PLM79_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOV87153.1"
                     /translation="MGQVAIIGLGNFGYYLGRELYLKGYDVIGLDIQQDVVQRAKNDL
                     SQGVVADSTDKEILLALGIGQVDLAVVTIGTNMLASILTTFHLKEIGVKKVFAKALSE
                     EHGQILVRMGADEILFPEKDLAVSLARRIENPNLLEYLPTVEDYGIFELDDPGALSGM
                     SLRQMDLINRFGIQVLAVRDREKDRLAFIPKGDYVVRGKDLLILLGTSQSMKRLKKEI
                     AGKSFGSRG"
     gene            complement(4593..5966)
                     /locus_tag="PLM79_12380"
     CDS             complement(4593..5966)
                     /locus_tag="PLM79_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOV87154.1"
                     /translation="MTRFLKHLSPSLYPVVSFLLLILTGSLVLTFLPTRSGPGISFID
                     ALFMVTSASCVTGLTVLDIGTEFTLWGQLTLLACIQLGGLGIMTFSTVMILALGRSIS
                     FRSRFVVQDVFAHSPRADFHILLRRVILFTAGIEAAGTLVLFIRFKDRLEFTDAWYSA
                     AFHSISAFCNAGFSLFSDNLAGFRGDVLVNLTVTSLIILGGIGFMVLHELVRSSRDWR
                     SWSYFWNQLSLHSKIVLFTTGLLLVGGTLFFLVCEWSNTLKDLPLREKLLASYFQSVT
                     PRTAGFNTLDYGRMSNITLLGTVMLMFIGASPGSTGGGVKTSTMGVFLAIIRARFKGS
                     DHTHVFKRSIPEETINRAFGTFVVSVCIVLLGTLGLLVSESAGVTLSQSRGQVIELLF
                     ETTSAFGTVGLSMGITPKLSSWGKFILVLIMFTGRLGPLVIATAIQPAQQKTGRFYYA
                     EERIMIG"
     gene            complement(6062..6838)
                     /locus_tag="PLM79_12385"
     CDS             complement(6062..6838)
                     /locus_tag="PLM79_12385"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOV87155.1"
                     /translation="MAAFQLGDWVLLTSQKGKKWLARVEDAPYSSHLGGIRMTDVVGK
                     EEGDCLETTKGAKLFLFRPTLQDYIYRMERQTQIIYPKDLAAIVFYGDVRPGDVILES
                     GLGAGALCLALLRAVGGRGTVISVEKRAKFARQAMANIHKFLNCKPENHWVVVADIQD
                     FSMTAKVDRIFLDLPEPWHAVGSLAGLLRPGGILVSLSPNVGQVQLMHRELKGNGFAN
                     VVTFELLQREWKVDELRARPVDRMIAHTGFITVARKVPVP"
     gene            7454..8464
                     /locus_tag="PLM79_12390"
     CDS             7454..8464
                     /locus_tag="PLM79_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOV87156.1"
                     /translation="MPILFGILLLSGSPCHARSLKAIVLTDAAGLGDKGFNDVCWQGV
                     LRAKKDFGISSQFLQSREQADYIANLSLAAQSADIVVTLGYLFSDAVREVVPRYPRVR
                     FIHIEGDVVGPNVVSYDFNSQEGGFLAGLVAGLYSKTRKVGVVGGVDIPPVEAYVSGF
                     RAGVMTAEKARGEPIEAVVASAGSFNDPVKGKSLAMAMIERKVDVVFRCAGNTGIGVL
                     EAVKQTQDVFLIAEDLDQDAEIPGRVLTSTLKRMDVAVYGAVRDIVEGRFSGGHHWLG
                     AKEGAVDITEMKYSRERFDAKDLARIRKARELLRGGTLKVPKRFGEVKDFPAPDLDTE
                     SS"
     gene            8485..10095
                     /locus_tag="PLM79_12395"
     CDS             8485..10095
                     /locus_tag="PLM79_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV87157.1"
                     /translation="MKPADFPSHDPVLSLRGVSKRFGSLWANRDISLDIRAGEVLAVV
                     GENGAGKSTLMNLLYGRLQPDSGEIHVHGRRVRFSHPRQALAAGIGMVHQELQIFPQL
                     TVLENIVAGSTQSRSPWVRHRERAMEIGSLCSNFGFDLPLEGTAGELPFARRQEIEIL
                     RALYRDARILILDEPTSLLAPPEVEKLFRFLGSLRTQGRTILLISHRLSEVFALADRI
                     GILRRGRLIGTYPVEEVSREEIAGRMIADSSIREPKADVPSADRFPLESEERDTGIPL
                     LRLQNLHTPSWSRDAGIRELSLEVYPGEILGVGGVVGNGQRSLAAAVAGIGPISRGRL
                     FLEGVEFTRSSIAERLEMGIRRLSANPPEETFAPHRSLWENLLLGFQRRPAHRKGGLL
                     RKAEILRWTIERLEEYEAVYSNPRKPLSTLSGGNRQKAALAHAFAGTPRLLILEQPGR
                     GLDVHSQERLHRRIRAMSSRGVSFLFFSYDLDELLSVSHRIAVLYRGRLAGIVSRRDA
                     SLQLLSRWMFGLPTSDGEDLRSREGPLQ"
     gene            10092..11210
                     /locus_tag="PLM79_12400"
     CDS             10092..11210
                     /locus_tag="PLM79_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV87158.1"
                     /translation="MTRPDTAIFRSLREAGRALSAPLAAMGCGLLVCSIIVFLSGTHP
                     WDFLAVTWKGSWGSGQAVAATLGKTTPLLLTGLAVSIAYQAGFLNIGCEGQLTLGALC
                     AATLAALDLPLPRFVLVPCLALAGACTGALWAYPACLLKQRRGVHEVITTLLLNYVAI
                     HLAQFLVLGPLGDGSAMGRTPEIPSKAALDPLWESGALGVTAAPPAALLLALGVQFWK
                     SRTVWGYEATMTGSNPAAALCAGISTDRLQRRLFLLSGAFAGLAGSLEILAVHHRFYN
                     AFSPGYGFDGITVAFLVNAAPGWLWLSSLLLAGLRSADKWLQILLGVSPNIIVVIQAV
                     LLLAAASQGRLRERAAALFERTRRKPGGPSFPAEGRAP"
     gene            11256..12167
                     /locus_tag="PLM79_12405"
     CDS             11256..12167
                     /locus_tag="PLM79_12405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV87159.1"
                     /translation="MTASHFIPLTLIKATPILLACLGGLLSELSGVVNFALEGMLLMG
                     AFGAVWASFVTGSPWIGLLGGMAGGMLVGLLHAGASLSLRANQIVSSIALNLLAAGGT
                     GMFLNQLFEVYGTSPAVPGLPTLAQSLSAFFPSSMPAGPWVRGLSLMVPMALTLGIAV
                     AALLKWSRWGLDLRACGENPYAAAGAGIPVLRMRFTAVVAGGALSGLGGAYLSIGELS
                     QFVEQMSHGRGYLAIAALILGRWHPVGALGAAVFFGFCEVLSEWLAVRWPRFPHQGFL
                     CLPYLLCVLVLFCPLGKRRPPSALGKL"
     gene            complement(12496..13257)
                     /gene="hisF"
                     /locus_tag="PLM79_12410"
     CDS             complement(12496..13257)
                     /gene="hisF"
                     /locus_tag="PLM79_12410"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOV87160.1"
                     /translation="MEPIKIMPCLDMKEGRVVKGINFVNIRDAGDPVENAAFYEKEGA
                     DELAMLDIAATLENRKTRLEWVKSVSSVIQIPLTVGGGISSMEDIELVFKAGASRVSM
                     NSAAVQNPDLVRQAARTYGSQKITVAIDGKRNPQMPSGFELVVSGGTRPVGKDAVQWA
                     GQCQDLGAGVILPTSMDGDGTRAGYDLEFTRAISDAVSLPVIASGGAGELEHFYEGVV
                     RGGAQILLAASVFHYRTLSIRQVKEYLKSRGLPVL"
     gene            13466..>14313
                     /locus_tag="PLM79_12415"
     CDS             13466..>14313
                     /locus_tag="PLM79_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV87161.1"
                     /translation="MLDKEKISKLRISQQDPGTPPPRRKRRWLPALLLAGIAAVAATV
                     LYQKGLLAPAVSVRTASAVLVYPSQAVTDFNASGYIVAQRKAAVSSKGTGRLETMEVR
                     EGSRVKDGDVLARLENDDLREERAQAAAQLNTAGAELARAQSELLTAEKHWNRLKGLW
                     YDRRAVAQSDYEAAEDRYLKAKAAIEAARANIAAQEANLRRISVLIEYTIIRAPFDGV
                     VLKKDADVGEVVAPFGSATNAKAAVVTMADMTSLMVEADVAESFLHKVREGQPCEIRL
                     DSIPDER"
BASE COUNT         2629 a         4559 c         4358 g         2767 t
ORIGIN      
        1 ctgttcatgt ttctggccat tgcctttctc gaagacaccg gctacctggc ccgggtcgcc
       61 tacatgctgg accgggtctt tcgaaccttc gggctccatg gaaattccgt gatggcctac
      121 gtcatttccg gcgggattgc ggggggctgc gccgtccccg gcgtcatggc cgcccggacc
      181 cttcgcagct cgcgcgagcg cctggccacc cttctcaccg ctccgttcat gaactgcggc
      241 gccaagctcc cggtgctcgc catgctcatc gccgcttttt tccccactcg tcatgcccag
      301 gtgatgtttc ttttgaccgt tctttcctgg atcctggccc tcctgctggc caagctcttc
      361 cgaaccacca tcctgcgggg gcccagcact ccgtttctcc tcgaacttcc tccgtaccgt
      421 ctgcccacgt tccgggggct cctcattcac acctgggagc gcacccggca gtatatccag
      481 aaagcaggca ccatcattct ggccatgtcc gtcctcttct gggcactcat ggcctttccc
      541 ggtctgccgg attcccaaaa gcggaggtac gaggaacaac gatccgaaat cctcgcccgt
      601 tttcccccgg acctccgaat tcctctcgag cggaactcga tccagcccaa agatcttcca
      661 tcggagctcg ttccatggta cgagcaactc aaggctgcgg atcgcgccga ggcccaggcg
      721 gccttgcagc attcctgggc tggaagaatg ggtatcgccc tcgagtccct gagccggtgg
      781 acgggatttg actggcgaac caacgtggcc ctggtggcgg gcttcgctgc aaaggagctc
      841 atcatctcca cctttggcac ggcctactcc atgggggtgg taacccagga ggaagggggg
      901 gccctgagcc gacagctggc gtcggacccc gggtggaatc ccctgagagc ctttgctctc
      961 atcttgttca tcatgctgta cgccccgtgc ttcgcgacga tcacctgcat cattcgggaa
     1021 tccggttcct ggaaatgggg gctgttttcg gcggctttca acaccggcgc ggcattcttg
     1081 atttcccttt tggtctacca gggcgggcgc tggctcggga tcgggacata aacgagggaa
     1141 gaacctatgt ggcagatggt tttggtgttg ctcgtggtgg ctgccgtgag cgtttacatc
     1201 gtccgccatt tcgtacggat cacccggtcc gagactccaa cgtgcggcca ttgtgccggg
     1261 tgcccctgca caccctcatc cacggtcccc cgggaatgca acccggagca gggcaatccc
     1321 tgcgcctgcg agaacccgcc ccaggaagga tgacggtttg gagcgacgtt gtgcaagatt
     1381 gtgcgggagg tcgagatgga gctgaccctg gagttgcccg gttctcctcc ccggagttcc
     1441 cgggtgagga tcggggaaga catcctcgag aacctgcccg aatccttgtc ttcccggtgg
     1501 gaaggctccc gcatcttctg gatctgggac cgggccgttc atgccctgtg gcaccccagg
     1561 ctgagagaga tggggtggcg gccctcctcg gaggatgaat ggattctctt tcccgcctcg
     1621 gagtccaaca agcgtctttc ccagctcgag gagctggccc gcaggctggt tcgagccggg
     1681 gccgaccggc gcagcgtcct tgtggccgta gggggcggcg tgacgggaga cgtggtgggc
     1741 tttctggcat ccgtctacat gcgggggatt ccccactgcc aggtccccac cactctgctg
     1801 gcccaggtgg acagcagcat cggggggaaa acgggcgtcg atcttcccga aggcaagaac
     1861 ctcctgggga cctttcacca ggctcattcc atctggatgg acattcgatt cctggaaacc
     1921 ctgcctcccg aggaatttcg ccagggtatg gccgaagtaa tcaagacggc catgatcgga
     1981 gacccgtccc tctgggaata tttggaatcc cattcggagg acgtccgatc ccgccgcagc
     2041 gagtcgctcc tgcgggtggt ctcctcctgc tgtcgcctca aggcccgggt tgtggaggcc
     2101 gacgagaggg agtccggcta ccgcatggtg ctcaacctgg ggcacaccgt cggacacgct
     2161 ctcgagaaga ccaccgggta cgccttccgc cacggggatg ccgtggccgt cgggatggct
     2221 gccgcggccc ggatttcgct gaaccgggga gaactttcca tgaagaatct tcaacggctg
     2281 gaaagcctct gccgtttctg gggacttccc accaccctcc ccgggggctc ccccccggac
     2341 gaaatccttg ccgccctatc cgccgacaag aagcggttcc tgggtgtgaa tcactttgtc
     2401 ctgcctcggg cgatgggaga ggtgactgtt cggtccgaca tcgctctcga agaagtccgg
     2461 gacgtcctcc gggccatgag ctcctcctga cccatcccgc accggggagg aattcttcct
     2521 gcgcctcgcg gggggtctca aggctgggct tttggttcat catggttact ttggttcatc
     2581 atggttacaa tgtgggtaaa gaaccggccc cggaagagca gggcttagcc gcccctggcg
     2641 gccgcaaaag ttccggcgaa ggatgtggat tggggagtga cggcgcggct atctcccccc
     2701 acccggccct cggtctcacg gcaaaatccc cggcaggagg tcccatggct gagcactcac
     2761 cacatcatcc accccctcgg tctgaggctt tgctgtggga catcctcgag tccgtcacgg
     2821 acgccatcgt caccatcgac gaggatcaca aggtcgttct ctgcaaccgg gccgcggaaa
     2881 agatgttcgg gtatcttccc gaagagatga tcggacaaga cgtttctccg ttgattccct
     2941 cctcgcaccg ggtcttgcac aaggggtacg tggaacgata cctggcaacg ggggtccccc
     3001 gggtcatcgg gatgctgagg gaatgccatg cccagcgcaa gaacggggaa tcctttcccg
     3061 tggagatttc ctattccgtt tcgaggtccg gggaacgcac ctatttcacg gcggtcatac
     3121 gggacatttc ccagcgcaag cagatggaac gggacatcca tttcatggaa aggctcgtgg
     3181 acatcggcaa agccgtctcc cagatcactc acgaaatccg caagcccttg atgctcatcg
     3241 gggggttcgc tcaccaactg aggagctgtg acggtttgca ggccgacgag aagtcccgcc
     3301 gcaagctgga catcatcctc aaggaagtgc agcgtctcga atcccttttg aacggcattc
     3361 gcctggccac acgcccgggt acctcgggaa ggaaacagct cctttccgtt cgcaagcttc
     3421 tcgaggagac cttcgagctg tttgaatcca ccctccggga aacccggatc cgattcaccg
     3481 ccgagctgga tccggacccc ctcatggtcg agggggactc ggaccagcta aagcaggtct
     3541 ttctcaacat gatgcaaaac gccgtggaag ccatgaacgg agtgggcact ctcaccgtag
     3601 ggacccggat cggggacgga acgatccagg ttctcatccg ggacagcggt cccggaatcc
     3661 ccgtcgaaat ccgggagagg atcttcgatc cctttttcac aaccaagacc gaaggcaccg
     3721 gcctgggtct cgccatttcc cgcaatatcg tccaggacca cggaggcgcc atctccgtgg
     3781 aatcctcgag ccggggaacc accttcctgc tgcagcttcc cccgggattt ccctagcata
     3841 ttctcgattc cgagacgctg tgcaaggggc accgcccacc accaaagggg gggattcgat
     3901 cagcccctgg agccgaaact cttgcccgca atctcttttt tcaaccgctt catggactgg
     3961 ctcgtcccaa gcaggatgag gaggtctttc cccctcacca cgtaatcgcc cttgggaatg
     4021 aaggccagtc gatccttttc ccggtcccgt acggccagga cctggatccc gaaccggttg
     4081 atcaggtcca tctgccggag ggacatgccc gacagggcac ccggatcgtc cagctcaaaa
     4141 atgccgtagt cctccaccgt cggcaaatat tcgaggaggt tcgggttttc gatccttcga
     4201 gccaggctga cggccaggtc cttttcgggg aaaaggatct cgtcggcccc catgcgaacg
     4261 aggatctgtc cgtgttcttc gctcaaggcc ttggcgaaca cctttttcac cccgatctcc
     4321 ttgagatgaa acgtcgtgag gatgctggcg agcatattgg tgccgatggt caccacggcc
     4381 aggtccacct gcccgatgcc cagggccaga aggatctctt tgtccgtgga atcggcgacc
     4441 actccctggc tgagatcgtt cttggccctt tggaccacgt cctgttggat gtccaggccg
     4501 atgacgtcat agcccttgag gtagagctcg cgacccaggt agtagccgaa atttcccaga
     4561 ccgataatgg cgacctgacc cattgaagga cctcacccaa tcatgattcg ctcttccgcg
     4621 taatagaatc gcccggtttt ttgctgcgcg ggctggatgg ccgtggcgat gaccagggga
     4681 cccaaccggc ccgtaaacat gatcaacacc agtatgaatt tgccccacga ggaaagcttc
     4741 ggggtgatcc ccatactcaa acccaccgtg ccgaaggccg aagtcgtctc gaagagaagc
     4801 tcgatcacct gtccccggct ctgggacaag gtcacccccg cggattcgga aacgagcaga
     4861 cccaacgttc ccagcaagac gatgcagacc gacaccacaa aggtgccgaa ggcccggttg
     4921 atggtctcct ccgggatgga ccgtttgaac acatgggtgt gatccgaccc tttgaaacgc
     4981 gcccggatga tggccagaaa tactcccatg gtgctggtct tcaccccgcc ccccgtggac
     5041 ccgggagagg ctccgatgaa catgagcatc accgttccca gcagagtaat gttgctcatg
     5101 cgtccgtaat ccagggtatt gaagcccgcc gttcgcggag tcaccgactg aaagtaggaa
     5161 gccaggagtt tttcccggag gggaaggtcc ttgagggtat tggaccattc acacaccagg
     5221 aaaaagaggg tcccgccgac cagcaggagg ccggtggtga aaagcacgat cttggaatgg
     5281 agggagagct ggttccagaa gtaagaccag gagcgccagt ccctggagga acgcaccaat
     5341 tcgtggagga ccatgaagcc gatgccgccc aagatgatca ggctggtcac ggtcaggttg
     5401 accaggacat ccccccgaaa acccgccagg ttgtcggaaa acaagctgaa cccggcgttg
     5461 cagaaggcgc tgatggaatg aaaggccgcg gaataccacg cgtccgtgaa ttccagccgg
     5521 tccttgaagc gtataaaaag cacgagagtg ccggcggcct cgatccctgc cgtgaagaga
     5581 atcactcggc gcagcaggat gtgaaaatcc gcccggggac tgtgggcgaa gacgtcctgg
     5641 accacaaacc gggagcgaaa ggaaatcgag cggcccaagg cgagaatcat gaccgtggaa
     5701 aacgtcatga ttcccagtcc ccccagctgg atgcaggcaa gaagagtcaa ctgtccccaa
     5761 agggtgaatt ccgtgccgat gtcgaggacg gtaagacccg tcacgcagct tgccgaggtg
     5821 accatgaaca gggcgtcgat gaaggagatg cccggcccgc ttcgggtggg caggaaggtg
     5881 agaacgaggc tgcccgtgag aatcagcagc aggaaactca ccactgggta gagggacggg
     5941 gaaaggtgct tgagaaatcg cgtcatccca aggtcctcga aatgaaccct cgcttccggg
     6001 cgaacgtcac ggggccacta tacagcggtc ccgagccctt gacaaatgtc gggtccatgc
     6061 cttatgggac gggcaccttg cgggccacgg tgatgaagcc ggtgtgagcg atcatgcggt
     6121 cgacgggccg ggcccgcagc tcgtccacct tccactcgcg ctgcaggagc tcgaaggtca
     6181 ccacgttcgc aaagccgttg cccttgagct cccggtgcat gagctgcacc tgccccacgt
     6241 tggggctgag actgaccagg attccccccg gcctgaggag gcctgcgagg gatccgacgg
     6301 cgtgccacgg ttccggcaga tccaggaaga tccggtccac cttcgcggtc atggaaaaat
     6361 cctggatgtc ggccaccacg acccaatggt tctcgggctt gcagttcagg aatttgtgaa
     6421 tgtttgccat ggcctgccgg gcaaacttgg ctcgtttttc cacggaaatg acggtcccgc
     6481 ggcctcccac ggcgcgcagc agcgccaggc acagggcccc ggcccccagc cccgattcga
     6541 ggatgacgtc cccgggacgc acgtccccgt agaagacgat ggccgccaga tccttgggat
     6601 agatgatctg ggtctgccgc tccattcgat agatgtagtc ctggagagtg ggccggaaaa
     6661 gaaagagttt ggctcccttg gtggtctcga ggcaatcgcc ttcctccttt cccaccacgt
     6721 ccgtcatgcg aattcccccc aagtgtgagg aatagggggc atcctcgacc cttgccagcc
     6781 acttctttcc tttctgggac gtcagtagga cccaatcccc caactggaac gcagccatga
     6841 tctccacctt gtccttcccc ggttgatttc cgacgcaaaa tcccctcccc gggcaggaag
     6901 ggcacccggg aaggagatcg aggtgcagtg ggaatttcca ccaccatttc atccgcagac
     6961 ggcatgccgc ggtatcccgg ccaaaccctt tcgggtccgg aaggcgaaaa gaggctcctc
     7021 gccgggcgaa tcccggcaaa cggtgggtgc accatagaga aaggcgaggc tttccgtcaa
     7081 cttgattttg tcccaccgga aagaacggcc ccggaaaagc tggtctctgc ggccccaaac
     7141 gtggtatcat tctttttggc ctgcacccat cgaggcctcc tttcgcacaa gaaggaccgt
     7201 gattccgaac aagactgtct ccccccgggg agaggcatgg aaagggcggg aagttccaat
     7261 ttgcggactc gcccgggaaa tccaaccggt cgcgccgtct ccctgagagg cccatggacg
     7321 ggggtgtagg gaggacgacg cccgcaccag ggacggacgg aaaggagtgc gaaccttgag
     7381 gaaatccacc ctgaatccgg atggtcgcaa aacccgtgga cttcccgttc atcgttgtct
     7441 ttggggagga atcttgccga tccttttcgg catcctgctt ctttcgggct ccccctgtca
     7501 cgcccgctcc ctcaaggcca ttgtcctcac ggatgcggcg ggactgggag acaagggctt
     7561 caacgacgtc tgctggcagg gggtcctccg ggccaagaag gatttcggga tttcttccca
     7621 gtttcttcaa tcgagggaac aggccgacta cattgccaac ctgagcctcg ccgcccagag
     7681 cgccgatatc gtggtgaccc tgggatatct cttttcggac gccgtccggg aagtggttcc
     7741 ccggtacccc cgggtccggt tcatccacat cgaaggggac gtggtgggcc ccaacgtggt
     7801 ctcttatgac ttcaactccc aggaaggggg attcctggcg gggctggtgg cggggctgta
     7861 ttcgaaaacc cgcaaggtgg gagtggtcgg cggagtggac attccaccgg tggaggccta
     7921 cgtgagcggc ttccgggcag gagtcatgac ggcggagaag gctcgcggcg aacccatcga
     7981 ggccgttgtg gcctcggccg ggtccttcaa tgacccggta aaaggcaagt ccctggccat
     8041 ggccatgatc gaacggaaag tggacgtggt ttttcgatgc gccgggaata cgggcatcgg
     8101 agtcctggaa gcggtgaagc aaacccagga cgtctttctc attgccgaag acctggatca
     8161 ggacgccgaa atccccggca gagtcctcac cagcactctc aaacgcatgg acgtggccgt
     8221 ctacggagcc gtccgggaca tcgtggaggg aagattttcg ggagggcatc actggctggg
     8281 agccaaagaa ggggccgtgg acatcaccga aatgaagtat tcccgggaac ggttcgatgc
     8341 caaagacctt gccagaatcc ggaaagcccg ggaactcctg cgtgggggaa ccttgaaagt
     8401 ccccaaacgg ttcggagagg tgaaggactt cccggcaccc gacctcgaca cggaaagttc
     8461 ctgatcctcc ccggcccgcc gcccatgaaa ccggccgatt tcccgtccca cgatcccgtt
     8521 ctttccctcc gcggggtttc caagcgattt gggtccttgt gggccaaccg ggatatttcc
     8581 ctggatatcc gggcggggga agtcctcgcc gtcgtcggtg aaaacggggc gggcaaatcc
     8641 accctcatga acctcctcta cgggcgcctg caaccggact cgggagagat ccatgttcac
     8701 ggcaggcgcg ttcgtttttc ccacccccga caggccctgg ccgccggaat cggcatggtt
     8761 caccaggagc tccagatctt cccccaactg acggtcctgg aaaacatcgt cgcgggctcg
     8821 acccaatccc gctccccctg ggtgagacac cgggagaggg ccatggaaat cggaagtctc
     8881 tgctcgaatt tcggcttcga ccttcccctc gaagggacgg ccggggagct gcctttcgcc
     8941 cgtcgccagg agatcgagat ccttcgagcc ctgtaccggg acgcgcgcat cctcatcctg
     9001 gacgagccca ccagcctgct ggctcccccc gaggtcgaaa agcttttccg atttctcggt
     9061 tcccttcgaa cccagggccg taccatcctc ctgatcagtc atcgactgag cgaggttttc
     9121 gccctggccg accgcatcgg catcctccgg cgcggtcgcc tgatcgggac ctatcccgtg
     9181 gaggaagtga gtcgagagga aatcgccggg cgcatgattg cggattcctc gatccgggag
     9241 cccaaggcgg acgtcccctc ggcggaccga ttccccctcg agtccgagga gagggacacg
     9301 ggaattcccc tgctccggct tcaaaacctt cacaccccct catggagccg ggacgccggg
     9361 atccgggagc tttccctcga ggtctacccg ggagaaatcc ttggggtggg gggagtggtg
     9421 ggaaacggac agcgatccct ggccgccgcc gtggccggga tcggacccat ctcccgggga
     9481 aggcttttcc tcgagggggt cgagttcacc cggtcttcga tcgcggaacg gctcgagatg
     9541 ggaattcgcc gcctttcggc caacccgccc gaggaaacct tcgcacccca ccggtccctg
     9601 tgggaaaacc tgctcctggg cttccagcgc cgccccgccc accggaaggg cggcctgctc
     9661 cgtaaggccg aaatcctccg gtggaccatc gaacggctcg aggaatacga agccgtatac
     9721 tcgaatcccc ggaaaccgct ttccactcta tcgggaggaa accgtcaaaa ggcggccctg
     9781 gcccacgcgt tcgcgggaac tccgcggctg ttgatcctcg agcagcccgg ccgcggcctg
     9841 gacgtgcatt cccaagaacg cctgcatcgg cggattcggg ccatgagctc ccggggcgtg
     9901 tccttccttt tcttttcata cgacctggac gagctgcttt ccgtcagtca ccgtattgcc
     9961 gtgctctatc gaggtcgcct ggctgggatc gtttctcgaa gggacgcttc cctccagctg
    10021 ctgagccgct ggatgttcgg cctcccgacg tcggacgggg aagatcttcg ctcacgggag
    10081 ggccccctcc aatgactcgg ccggatacgg caattttccg ttcgctgaga gaagcgggcc
    10141 gggcgctttc ggccccgctt gcggccatgg gatgcgggct cctggtctgt tccatcattg
    10201 tcttcctttc gggaacccat ccctgggatt tcctcgcggt cacctggaag gggtcgtggg
    10261 gaagcggcca ggcggttgcg gccaccctgg gaaagacgac tcccctgctg ttgaccggcc
    10321 ttgccgtgag catcgcctac caggcgggct tcctcaacat cgggtgcgaa gggcagttga
    10381 ccctgggggc actctgcgcc gcgacccttg ccgccctgga tcttccgctc ccgcggtttg
    10441 tcctggtgcc gtgcctggcc ctagcggggg cctgcaccgg cgccctgtgg gcgtatcccg
    10501 cctgcctgct gaaacagagg cgaggggtcc acgaagtcat cacgaccctg ctcctgaact
    10561 acgtggccat ccacctggcg caattcctgg tgctggggcc cctgggagac gggtcggcca
    10621 tgggccgaac ccccgaaatc ccctccaaag ccgccctcga tcccctctgg gagagcggtg
    10681 ccctgggagt caccgccgca cccccggcgg ccctcctcct ggccttgggg gtacagttct
    10741 ggaaatctcg aaccgtgtgg ggatacgaag ccaccatgac cgggtccaac ccggcggcgg
    10801 ccctttgtgc ggggatttcc acggaccgcc tgcaacgacg gctgtttctc ctgagcggag
    10861 ccttcgccgg attggcgggc agcctggaaa tcctggcggt ccatcatcgc ttctacaacg
    10921 ccttttcccc aggctacggc tttgacggca tcaccgtcgc cttcctggtg aacgcagctc
    10981 ccggatggtt gtggctgagc tctctgctgc tcgccggcct gaggtctgcg gacaagtggc
    11041 tccaaatcct cctgggcgtc tcccccaaca tcattgtcgt gatccaggcc gtgctgctgt
    11101 tggctgcggc ctcccagggg agactgcggg agcgggcggc cgccttgttc gagcgtacaa
    11161 gacgcaagcc cggaggaccc tccttcccgg cggaaggccg agccccgtag ccggaggtgg
    11221 ttcgaacacc tatacaagga gtccccgttt catccatgac ggcttctcat ttcatcccgc
    11281 tgaccctgat taaggcgacc cccatcctgc tggcatgcct cgggggcctc ctctccgagc
    11341 tttcgggagt ggtgaatttc gccctcgagg gcatgttgct catgggggcc ttcggggcgg
    11401 tctgggcttc cttcgtgacc ggttctccgt ggatcggcct gctgggagga atggcggggg
    11461 gaatgctcgt gggactcctc catgccgggg cttccctgtc ccttcgagcc aaccagatcg
    11521 tgagctccat tgccctgaac ctgctggccg cggggggcac cgggatgttc ctgaaccaac
    11581 tcttcgaggt ctacggaacc tctcccgccg taccgggtct tcccaccctc gcccagagcc
    11641 tctccgcttt ctttccttcc tcgatgcccg caggaccctg ggtccggggt ctttctttga
    11701 tggttcccat ggccttgacc ttgggaatcg ccgttgccgc actcctgaag tggagccggt
    11761 ggggattgga cctgcgggcc tgcggcgaga atccttatgc ggcggcgggg gcgggcattc
    11821 ccgtgcttcg catgcgcttc acggccgttg tggcgggggg ggcattgtcg gggctcgggg
    11881 gggcctacct gtccatcggc gagttgtccc agtttgtgga gcagatgagc cacggcaggg
    11941 gctacctggc catcgcggcc ctgatcttgg gccgatggca tcccgtgggc gctttggggg
    12001 cggcggtctt cttcgggttc tgcgaagtcc tgtccgagtg gctggccgtt cggtggcctc
    12061 gtttccccca ccagggattc ctgtgcctgc cgtatcttct ttgcgtcctg gttctttttt
    12121 gccccctggg caaacgccga cctccttcgg ccctgggaaa gctctaggcc ccatcctccg
    12181 ggtcgccggg agagaggcgg cccgccgggg gcgtggttca tggcgatttg agacccatgg
    12241 gttcgtcgtc ccgagggtga agacgggccc ctgagttgag cgaatccgaa agcatcaggg
    12301 ctcatgcccg cgacggcggg caccccgcag gtatgcacgc tctttccgga ttcccgcctg
    12361 cgcgggaatg aagaaacggg actcgtacca gtcaaatcgc tcaatccagg gggcagatgc
    12421 gcccttttca agggtttccg ggtccgattc cccggttccc gcccggaaaa ccggacgaga
    12481 ttcggagcac gaaactcaga ggaccggcaa tccccggctc ttgaggtact ctttcacttg
    12541 ccgaatgctc aaggtacggt agtggaacac ggaggcggcc agcaggattt gggccccgcc
    12601 ccggaccacc ccctcgtaga aatgctcgag ctccccggcc cctcccgagg caatgacggg
    12661 aagactgacc gcgtcggata tggcccgggt gaattcgagg tcatagcccg cccgggtccc
    12721 gtccccgtcc atgctggtgg gaaggatcac cccggctccc aggtcctggc actgcccggc
    12781 ccattggacg gcgtccttcc ccacgggccg ggttcctccg gacaccacca gctcgaatcc
    12841 cgaaggcatt tgagggtttc gcttgccgtc gatggccacg gtgatctttt gggagccgta
    12901 ggtacgggcc gcctggcgca ccaggtccgg gttttggacc gcggcactgt tcatcgagac
    12961 ccgggaagcc ccggccttga aaaccagctc gatgtcttcc atgctcgaga tcccgccgcc
    13021 cacggtcagg ggaatctgaa tcacggaaga cacgctcttg acccactcga gacgggtctt
    13081 ccggttctcg agggtggccg caatatcgag catggccagt tcgtcggctc cctccttttc
    13141 gtagaaagcg gcgttttcca ccgggtcccc ggcgtctcga atgttcacga aattgattcc
    13201 cttgaccact cgtccttctt tcatgtccag gcatggcatg attttgatcg gttccatgac
    13261 aattcccttc taccggctgc acggcctgcc gacgggcaag gcgctgtttc gcgactcttt
    13321 tggggccatc ccctcgttct ccaccgccac caagatgtgt tgacattcca acggggctca
    13381 gtttacaaac ttccgacctg atgaaaaagc cggaaattct cccgggccgc ggaaaacaac
    13441 cgatcccttg acgaggggtg ggaccgtgtt ggacaaggaa aagatttcaa agctgagaat
    13501 ttctcaacag gacccgggaa ctcctccgcc caggagaaag cgaaggtggc tcccggcgct
    13561 gctcctggcg gggatcgcgg cggtagccgc caccgtcctc taccaaaagg gactcctggc
    13621 tcccgccgtc agtgtccgga ccgcctccgc ggtgctggtg tatccttccc aagccgtcac
    13681 cgacttcaat gccagcgggt atatcgtggc ccaacgcaaa gccgccgtat cgtccaaggg
    13741 gaccgggaga ctggaaacca tggaggtgag ggagggcagc cgggtcaagg acggggatgt
    13801 gctggcccgc ctcgaaaacg acgacctgag ggaagagcgc gcccaggcgg cggctcagct
    13861 gaacaccgcc ggggccgagc tggcccgggc ccagtcggag ctcctcaccg ccgagaagca
    13921 ttggaaccgg ctgaagggcc tctggtacga ccgccgggcc gtggcccagt cggattacga
    13981 agccgcagag gatcggtatc tcaaggcaaa ggccgccatc gaggccgccc gggccaatat
    14041 agcagcccag gaagccaatc tgcgccgaat ttcggtgctc atcgaataca cgatcattcg
    14101 agcccccttc gacggggtgg tcctcaagaa ggacgcggac gtgggcgagg tggtcgcccc
    14161 cttcgggtcc gccaccaacg ccaaggccgc agtggtcacc atggcggaca tgacctctct
    14221 catggtcgag gccgacgtgg ccgaatcctt tctgcacaag gtccgtgagg gtcagccctg
    14281 cgaaatccgc ctggattcca tccccgacga gcg
//
LOCUS       DAOOVC010000101        13874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2953_length_13874_cov_6.474347, whole genome shotgun sequence.
ACCESSION   DAOOVC010000101 DAOOVC010000000
VERSION     DAOOVC010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2953_length_13874_cov_6.474347"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..433)
                     /locus_tag="PLM79_12420"
     CDS             complement(62..433)
                     /locus_tag="PLM79_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87162.1"
                     /translation="MTDRTLRALLGAAAVSLVLGRLLYWLGELWGATAAMAGAGIAAL
                     VSMICARMARVGVGNVAWFLVPTLLFTIVPAAYKGWKFFMRETSIPDRMWELAPILLS
                     FVIPLGIILLVYLQLRRRESG"
     gene            complement(528..1064)
                     /locus_tag="PLM79_12425"
     CDS             complement(528..1064)
                     /locus_tag="PLM79_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HOV87163.1"
                     /translation="MKHHAFSLEPFSSAMPLPIRITGSVFRDSDLLTFRSTLSGLPAD
                     AVIPEAADRAVRKHHLWEETCFELFLARKGSTPYWEFNLSAAGHWNAYRFSDYRQGMQ
                     EEETFGTLPFEVQRTVRSVTVTLEIELDFMHPGDHAIEAGVAAVVRHGGGTMTYWALT
                     HCGPQPDFHRRESFLLTV"
     gene            complement(1061..2158)
                     /locus_tag="PLM79_12430"
     CDS             complement(1061..2158)
                     /locus_tag="PLM79_12430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOV87164.1"
                     /translation="MEILFSAADRFKAKGTVLDVRPHGNGNIHDTFLVSLDFPETRCF
                     ILQRVNTRVFGRPELVTANIRTLTDHVGKRLEREAPNSRQRWEIPRILPDREGSDHWI
                     APDGSFWRAMSFIESARSFETVQGAEHALEVGYGLGTFHRLIHDLPAERLADTLRGFH
                     VTPHYLQRFESVLAARPPGLSPEIRYALKFVEDRKAGVGVLEDARTRNRLRLRPIHGD
                     PKVDNILIDPETAQAVAMIDLDTVKPGLIHTDIGDCLRSCCNPSGEETQDWEAVRFEP
                     DLCRSILQGYLSTAGGFLTADDHDFLYDAVRLIPFELGLRFLTDHLEGDVYFKVRYPG
                     HNLVRALVQFKLTESIESLSGPLRAVLRELR"
     gene            complement(2220..3239)
                     /locus_tag="PLM79_12435"
     CDS             complement(2220..3239)
                     /locus_tag="PLM79_12435"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV87165.1"
                     /translation="MRMPRRQTRPTFRAFLLVFVLCTLASPAVASQDDPLRSWNEGPA
                     KHAVVDFVQRVTAPGGKDFVPEPERIAVFDHDGTLWAEQPLYVQDVCILDRIKALAPE
                     HPEWKDIEPFKSALAGDIKAVLTGGGPALLELVVAATAGLTSAELEAMAKNWISRARH
                     PRFDRLYTDLAYQPMLELLGYLRANGFKTFIVSGGGIHFLRSFAERVYGVPPEQVVGS
                     SLKKKFELRDGHPVIVELPEVQMVNDGLGKPVGIHLHIGRRPIAAFGNSDGDLAMLQW
                     TMAGDGPRFALLVHHTDGEREWAYDRTSMVGRLDKALDEAVAKGWAIVDMKRDWKVVF
                     PFEKR"
     gene            complement(3264..4295)
                     /locus_tag="PLM79_12440"
     CDS             complement(3264..4295)
                     /locus_tag="PLM79_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOV87166.1"
                     /translation="MHQSRFFHISPSGELARFTAIEEAPARTREGGFVWLDFSHPTRE
                     ELLPLVDLFALHPLAVEDCLDEKQLPKMEDYPRNTFFVFNTFDFSHHTLSVHELDVVL
                     GDRFLITVRANAADDQALLQDLPRILERNIENARQGPAFLLHVLLDQIVDRKFLAIEA
                     VEEDLDSAEETILADLARFNPAELLHLRRDLLTLRKTLFHEREILVKICRKDCPFISD
                     KAVYLYRDIYDHLTKSFELTESCRDIVTSLMEMYLSMLNNHMAKSANETNVTVKRLTF
                     ITTVFMPLTFLTGVLGMSEFSMIIGPENWKTGYPIFLAATAIVAVLNYYLLKWLDRPD
                     SSRTGPQAP"
     gene            complement(4357..7056)
                     /locus_tag="PLM79_12445"
     CDS             complement(4357..7056)
                     /locus_tag="PLM79_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV87167.1"
                     /translation="MFQTLPRKLRQVAEDDPRKVAVVYRDREERIEIPYGDLLESSLG
                     VASWLQARGVARGDRVALILENRPEWPMSYFGILFSGAVAVPIDPQSKPDIVSYILET
                     TRATFVFTSSGLSWKNTVLPSSLKGFVSVGGPNPSLPHAVSFSEIPRVVSTSADFPEA
                     GLEDLASIIFTSGTTGLPKGVMLTHKNFYANYLGVVRLDAVTSQDNFLSVLPLHHAFP
                     FMANLLTPLFYGARITYVSTLRAEVIFQCLKEENVTVLALTPQVLQFFYHGIQQRIRG
                     MPLGSGRLLFRLLDFTWKRSRRTGKNLAEPILSRIRAGFGSRFRFFVSGGAKLDEDLA
                     RSFFKLGLMVLEGYGLTETSPVVSINPPQAPRIGSVGKPLWGVEVRIFQPDADGVGEI
                     LLRGANVMKGYYRNEEATREAVRDGWFHSGDLGCLDADGYLYVRGRIKDLIVLSSGKN
                     VSSEEVAAHYAQAPSIKEIFVMAAPTQDRLVALVVPDFQYFRESGETDLYGKIKWDLE
                     YYSQKIESYKRIRGFVLHHKELPKTRLGKIERYRVEEIYREKIRETRGKTQPPPEESL
                     SPTARRVLGVLEARTEIREISPDDHLELTLGLDSLGRVELLTSMEKEFGVRIEESRFH
                     EVFTVDELVRLVESLEKQGGAEVRESARSWLEVLQARPPESLLRKVGIVPSWSARAVT
                     LFMAVILDPLFSILFRLRVHGREHLPHPPYILSPNHASYMDGFLVFSSVPFSQRLRLY
                     FQGYRIIFENPVVRRLLKLIRVIPVDSARSLTEAMQASAHVLRHGKVLCVFPEGARTV
                     GGEMQPFKKGAAILAKELNVPVVPTRIVGSYEAWSPTRTFPRPSPIGVFFGRPRSWQE
                     LRHRGWELDPNLGEYDAVTRGLREEVLRLGPEPPSESRSIKTN"
     gene            7803..7949
                     /locus_tag="PLM79_12450"
     CDS             7803..7949
                     /locus_tag="PLM79_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87168.1"
                     /translation="MAIVPAIIGGGLFFHLFENWTGVVVWEIVLLFLLSLVISKGDKR
                     AAAH"
     gene            7961..8164
                     /locus_tag="PLM79_12455"
     CDS             7961..8164
                     /locus_tag="PLM79_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOV87169.1"
                     /translation="MECKQEQNLKNCNCSYDPCARKGTCCDCLRYHLKSRQLPGCCFP
                     EKAERTYDRSFEHFARLVAEKKI"
     gene            8548..9810
                     /gene="hisS"
                     /locus_tag="PLM79_12460"
     CDS             8548..9810
                     /gene="hisS"
                     /locus_tag="PLM79_12460"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOV87170.1"
                     /translation="MERIQAVRGMNDILPGEIGWWQQVEEAARRIFQDFGYVEIRTPV
                     LEKLELFTRSIGENTDIVEKEMYAFPDRKGEWLALRPEATASIVRSFIQNDLYADPLA
                     RKFYSMGPMFRHERPQKGRYRQFHQVDVEAFGIDDPMLDAEIMFMLRLFLQKVGLAGV
                     GLHINSLGCRDCRKSYRESLRVFLASQAEGLCPDCLRRREVNPLRVLDCKVERCKTVL
                     ETAPVLLETLCDPCRDHFSRVQDYLSRLDTPFAVDPRMVRGLDYYMRTTFEVVTDRLG
                     AQSAVGGGGRYNGLIRDLGGPDLPGIGFAVGMERLVLLLQQQGGMPRRNARFFVATLG
                     SAAREEGFLLVQRLRSAGIGAELDYEGRSLKSQMRRADKLGARFVFILGEDELGRGEI
                     QLRDMREKNQRIVPLGEAVRHARTLPTG"
     gene            9903..11762
                     /gene="aspS"
                     /locus_tag="PLM79_12465"
     CDS             9903..11762
                     /gene="aspS"
                     /locus_tag="PLM79_12465"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOV87171.1"
                     /translation="MDQLETAREDSRSAASVEAGRELKPDSLEDWERSHSCNVLGPGD
                     VGKVVVLMGWVHRRRDHGGLIFVDLRDREGVTQVVFDPQHDVEAHARAHVLRNEFVIA
                     VEGTVRPRPEGMTNPKLVTGEIEVVISRLKILNPSRTPPFQIEDDLDVGENIRLRYRY
                     LDLRRPRLYGNLLFRHRAAQLTRNYLSERGFLEVETPVLTKSTPEGARDYLVPSRVNP
                     GKFYALPQSPQLFKQLLMVAGFDRYFQIVKCFRDEDLRADRQPEFTQVDMEMSFLREQ
                     TVYDLIEGWIVRIFGELLELNLPIPFPRMTYADAVDRYGTDRPDLRFGLQLGDITDLA
                     SASEFRVFRQAVERGGLVKVVRLPGGSGLSRKELDDLIDFVKVFGAQGMAWVKIQPDG
                     WQSPVTKFLSEEVRTGIARRLGAETGDILFFVADQRKVVHDALGNLRVRLGQQAGMVD
                     PREYRFVWVTHFPMFEWDQDEKRYTSVHHPFTAPLEEDLDLLETAPERVRSRAYDLVL
                     NGIEIGGGSIRNHRRDIQERIFRALGLTDREVEEKFGFLLEALHYGAPPHGGIAFGLD
                     RLIMLLTGSGSIRDVIAFPKTQKATCLMSGAPSEPDVRQLLELCVKVEKERRS"
     gene            11767..13599
                     /locus_tag="PLM79_12470"
     CDS             11767..13599
                     /locus_tag="PLM79_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL-associated domain-containing protein"
                     /protein_id="HOV87172.1"
                     /translation="MARWNSKKKVLDHEHSRFWRHSLMEVDEPNLMREIFPYEEVPRI
                     DFDHKLLPIDPAEDMFITDTTFRDGQQARPPYTVKQIGAIFDFLHRLSGPNGVIRQSE
                     FFLYTDKDRKALDLCRSKGYRYPEITGWIRAVESDLRLVAEAGLPETGILTSVSDYHI
                     FLKLKADRKGVMAKYLSIVRAALDKGIVPRCHFEDVTRADVYGFCVPFAIELMKLREE
                     SGIDIKMRLCDTLGFGVTYPGSALPRSVPKLVRAFIDDAGVPGHLLEWHGHNDFHKVL
                     VNTATAWLYGCSGANGTLLGFGERTGNAPIEGLIMEYIGIKGSVHGIDTTAVTDAVEY
                     FKEELGYRIPPNYPFVGEEFNATSAGIHADGLVKHEEIYNIFDTGKLLKRPISVIITD
                     KSGVAGVAYWVNNRLKLEGDRRLDKRHPGVLKIHKRVMKEYEQGRNTSISNEELERWA
                     RRYLSEYFVSEFDQLKQKAYALAAHMVEEVVESEAVRSMDSGRQEAVLQTLLDLNPFI
                     QYIYITDAQGIKITRNITHIVDKAKYESARVGEDLSDRPWFIEPMKNGRVHLTDFYTS
                     RYTGALCITVSAPIRNEVEEIVGILGMDMRFEDLAKMEADGDAF"
     gene            13622..>13874
                     /locus_tag="PLM79_12475"
     CDS             13622..>13874
                     /locus_tag="PLM79_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87173.1"
                     /translation="MKTASCSQGVRAIGFQGRSGLRLAVGFGFLVLVLLESCAKPPPP
                     SPTIPPPPGLRSQKPYQIRGIWYYPLPTAEGYVEEGVASW"
BASE COUNT         2759 a         3946 c         4484 g         2685 t
ORIGIN      
        1 ggagggcctg aacccatcgg cctgcccggg tcgggaaaca tcccccgggc aggcccccaa
       61 tctaccccga ttccctcctt cgcagctgca ggtagaccag caggatgatt cccaggggaa
      121 tcacgaagct caaaaggatg ggggccagtt cccacatccg gtccgggatg ctcgtctctc
      181 tcatgaagaa tttccatccc ttgtatgccg ccggaacgat ggtgaaaagc agggtgggga
      241 ccagaaacca cgccacgttc cccactccga ccctggccat tcgggcgcag atcatgctca
      301 ccagggcggc gattcccgcc ccggccattg ccgccgtagc tccccaaagc tcacccagcc
      361 agtaaaggag ccggcccagg acgagagaaa cggcggcggc acccaggagt gccctgaggg
      421 tcctgtccgt catggcagtg cgctcctttc ttccgacgcg gcattagggt cctcggtcgt
      481 gggccgagga aaggatttgg aaaactcctg cactctttcc cctttcttca gaccgtcagg
      541 aggaaactct cccgccgatg aaagtccggc tgaggtccgc aatgggtcag ggcccagtac
      601 gtcatggtcc cgcctccatg ccggactacg gccgccaccc ccgcttcgat ggcgtgatct
      661 ccggggtgca tgaaatccag ttcgatctcg agggtgacgg tgacggaacg gacggtcctt
      721 tgaacctcga agggcaaggt cccaaaggtt tcttcttcct gcatgccctg tcgatagtcg
      781 gaaaagcggt acgcgttcca gtggcctgcg gcggagaggt tgaattccca gtatggggtg
      841 gatcctttgc gggccaggaa gagctcgaag caggtctcct cccacaggtg gtgctttcga
      901 acggcccggt ccgccgcctc cgggatcacc gcatctgcgg gaaggcccga gagggtgctc
      961 ctgaaggtga gcagatcgga gtcccggaaa acgctcccgg tgatgcggat gggtaacggc
     1021 atcgccgagg aaaacggctc gagggaaaac gcatgatgct tcatcgcaac tcccggagga
     1081 cggcccggag tggaccggac agggattcga tgctttcggt gagcttgaac tgcaccaggg
     1141 ctcgaaccag gttgtgcccc gggtatcgga ccttgaaata gacgtctccc tcgaggtgat
     1201 ccgtgagaaa tctcaggccc agttcgaacg ggatgaggcg aacggcgtcg tagaggaaat
     1261 cgtggtcgtc tgccgtgaga aagcccccgg ccgtggagag atacccctgg aggatggatc
     1321 ggcacagatc gggttcgaag cggaccgctt cccagtcctg ggtttcttct cccgacggat
     1381 tgcagcagga acggaggcag tcgccgatgt ccgtatggat caatccgggc ttgaccgtat
     1441 ccaggtcgat catggccacg gcctgcgccg tctccgggtc gatcaggatg ttgtctacct
     1501 tgggatcgcc gtggatggga cgaagacgaa ggcgattgcg ggtgcgggcg tcttcgagga
     1561 ccccaacccc ggcctttcgg tcctccacga acttcaaggc ataccgaatc tccggagaaa
     1621 ggccgggagg tcgtgcggcc aggacggatt cgaagcgttg caggtaatgg ggagtgacgt
     1681 ggaagcccct gagcgtgtcc gccagtcgtt ccgccggaag gtcgtgaatg aggcggtgaa
     1741 atgttcccag cccgtagccg acctccaggg cgtgttccgc tccctgcacg gtttcgaagg
     1801 agcgggccga ttcaatgaag ctcatggccc gccagaagga cccgtcgggt gcgatccaat
     1861 ggtccgagcc ttcccggtcg ggcaggatgc gggggatttc ccagcgctgc cgggagttcg
     1921 gggcttcgcg ctcgagccgt ttgcccacat ggtccgtgag ggttcgaatg tttgccgtga
     1981 caagttcggg gcgaccaaac acgcgggtgt tcacccgttg gaggatgaag caccgggtct
     2041 ccgggaaatc gaggctcacc aaaaaggtgt cgtgaatgtt cccgtttccg tggggccgaa
     2101 cgtcgaggac ggtacccttc gctttgaacc ggtctgcggc ggaaaacaga atttccacaa
     2161 gctctcctca aaaaggtccc ggggcctact ggacgcgggg tcgttccaat cctgactcat
     2221 caccgcttct cgaaggggaa aacgaccttc cagtcccgtt tcatgtccac gatggcccag
     2281 cccttggcga cggcttcgtc cagggctttg tccaggcggc cgaccatgga ggtgcggtca
     2341 taggcccact cgcgttcccc atcggtgtgg tgcacgagga gggcaaagcg gggaccgtct
     2401 cccgccatgg tccactggag catcgcaagg tctccgtcgg agttgccgaa agcggcgatg
     2461 gggcgtcgtc ctatatggag gtgaatcccc acgggtttgc cgaggccgtc gttgaccatc
     2521 tggacttcgg gcagttccac gatcaccgga tgtccatccc gcagctcaaa cttcttcttt
     2581 aggctgcttc ccaccacttg ctcgggaggc accccgtaga cccgttcggc aaaggagcgc
     2641 aggaagtgaa tgcccccccc gctgacgatg aaggtcttga agccgttggc gcgaagatag
     2701 ccgagcagtt ccaacatggg ttggtaggca aggtccgtgt aaagacggtc gaaccgcggg
     2761 tgcctggccc gggaaatcca gttcttggcc atggcttcca attctgcgct ggtcagaccg
     2821 gcggtggcgg caacgaccaa ctcgagaaga gccggtcctc ccccggtgag caccgccttg
     2881 atatccccgg ccagggcgga tttgaagggc tcgatatcct tccattccgg gtgctcgggc
     2941 gccagcgctt tgattcgatc gaggatgcac acatcctgga cgtagagagg ctgctcggcc
     3001 cacagggtcc cgtcgtggtc gaacacggcg atgcgctccg gctcggggac aaaatccttt
     3061 cccccgggag cggtcacgcg ctgaacgaag tctacaacgg cgtgcttggc cgggccttca
     3121 ttccatgaac ggaggggatc gtcctgggag gcgaccgccg gggatgcaag ggtgcacagc
     3181 acgaaaacca ggaggaaagc gcggaaggtc gggcgggtct gtcttcgggg cattctcatg
     3241 gaaagtttcc tctttcctcg ggttcagggt gcttgtgggc cggtccggct tgagtccggc
     3301 ctgtcgagcc atttcaacaa gtagtagttc aggaccgcaa cgatcgccgt tgccgccagg
     3361 aagatcggat accccgtttt ccagttctcc gggccgatga tcatggaaaa ctcggacatg
     3421 cccaggaccc ccgtcagaaa ggtcaagggc atgaacacgg tggtgatgaa ggtcaggcgc
     3481 ttcacggtca cgttggtttc attggcggat tttgccatgt gattgttgag catggacagg
     3541 tacatttcca tgaggctggt gacgatatcc cggcaggatt ccgtcagttc gaaggacttg
     3601 gtcaagtggt cgtagatgtc ccggtagaga taaacggcct tgtcggagat gaacgggcaa
     3661 tccttgcggc agattttcac gaggatctcg cgttcgtgaa agagagtttt gcgcagagtc
     3721 agcaggtcgc ggcgcaggtg gaggagttcg gccggattga atcgggcaag atcggccagg
     3781 atggtttctt cggcgctgtc caggtcttcc tccacggctt cgatggccag gaacttgcgg
     3841 tccacgatct gatccagcag gacgtgcagg aggaatgccg gaccctgccg ggcattctcg
     3901 atgttccgct cgaggattcg aggaaggtct tggagcaggg cctgatcgtc tgcggcgttt
     3961 gcgcgaacgg tgatgagaaa ccggtccccc aggaccacgt ccaattcatg cacggacagg
     4021 gtgtgatggg agaaatcgaa ggtgttgaac acgaaaaagg tattccgggg atagtcttcc
     4081 atcttgggaa gctgtttttc gtccaggcaa tcctcgacgg caagaggatg cagggcgaaa
     4141 agatccacca gggggaggag ttcctcacgg gtcgggtgtg aaaaatccag ccagacgaag
     4201 cctccctccc gggtgcgggc cggggcctcc tcgatggcgg tgaatcgagc gagttcgccc
     4261 gagggggaaa tatgaaagaa ccgggattgg tgcatggcct tcggagtgcc tccaatgtgg
     4321 tttcacgacc cttctaatcg gttctccgtt cccctgtcaa tttgtcttga tggaacggct
     4381 ctccgagggg ggttccgggc cgagtcggag aacctcttcc cgaaggcccc gggtgacggc
     4441 gtcgtattcg cccaggttcg gatcgagttc ccagcccctg tggcgcaact cctgccacga
     4501 ccggggcctg ccgaagaaaa ccccgatggg tgagggccgg gggaaggtcc gggtcgggct
     4561 ccaagcctca taggaaccca cgatgcgggt ggggaccacg gggacgttca gctcctttgc
     4621 caggatggcc gctcccttct tgaacggctg catctcgcct cccaccgttc gtgcgccttc
     4681 cggaaaaacg cacaggacct tgccgtgacg cagcacatgg gccgatgcct gcatggcttc
     4741 cgtcaggctt cgggccgaat ccacgggaat tacccggatg agctttagaa gtctgcggac
     4801 cacggggttt tcaaaaatga tccggtagcc ctgaaaatag agccgcagcc tttgggagaa
     4861 aggaacggag ctgaagacga gaaaaccgtc catgtagctg gcatggttgg gactgagaat
     4921 gtaaggcgga tgcggcaggt gctctcttcc gtgcactcgg aggcggaaga ggattgaaaa
     4981 caggggatcc agaatcacag ccatgaaaag agtcactgcc cgggccgacc agctcggaac
     5041 gatcccgact ttgcgaagaa gggactccgg cggtcttgcc tgcaggactt ccagccagct
     5101 tcgggccgat tcccgaacct cggctcctcc ttgcttttcc agggattcca ccagccggac
     5161 gagctcatcc accgtgaaga cctcgtgaaa ccggctctcc tcgatgcgga ccccgaactc
     5221 cttctccatg gaggtcagca attccacccg acccagggag tccagcccca gggtcagctc
     5281 gagatgatcg tcgggggaga tctctcggat ttccgtccgg gcctccaaaa cgcccaggac
     5341 tcttcgggcc gtaggggaca ggctttcttc ggggggtggc tgggtctttc cccgggtttc
     5401 ccggatcttt tcccggtaga tttcttccac cctgtagcgt tcaatctttc ccagacgagt
     5461 cttgggcagc tccttgtgat ggagcacaaa cccgcgaatt cgcttgtagg attcgatctt
     5521 ctgcgagtag tattcgaggt cccacttgat ctttccgtag aggtccgttt cccccgattc
     5581 ccgaaaatat tggaaatccg gaaccaccag ggccaccagg cggtcctggg tgggagcggc
     5641 catgacgaag atctccttaa tggaaggtgc ctgggcgtag tgggcggcga cttcttcgga
     5701 agagacgttc ttgcccgaac tgagcacaat gaggtccttg atacggccgc gaacgtagag
     5761 gtatccgtcg gcatccaggc aacccaggtc cccgctgtga aaccaaccgt cccggacggc
     5821 ctcccgggtg gcctcctcgt tgcggtagta tcccttcatc acgtttgcgc cccgaaggag
     5881 gatctctcct accccgtcgg cgtcgggttg gaaaatcctc acctcgaccc cccacagggg
     5941 ctttcccacg gacccgatcc gtggggcttg gggcgggttg atgctcacca cgggagaggt
     6001 ttccgtaagg ccgtagcctt ccaggaccat cagtcccagc ttgaagaagc tccgggcgag
     6061 atcctcgtcc agtttcgctc ctcccgagac gaagaagcga aatcgagaac caaacccggc
     6121 gcgaatccgg gaaaggatgg gttcggcaag gtttttcccg gtgcgccggg atcgtttcca
     6181 ggtgaaatcc agcaaccgga aaaggagtct ccccgagccc aaaggcattc ctcgaatccg
     6241 ctgttggatg ccgtggtaaa agaattggag cacctggggg gtaagagcga ggacggtcac
     6301 gttctcctcc ttcaagcatt gaaagatgac ttccgccctg agggtgctca cataggtgat
     6361 ccgagccccg taaaagaggg gggtgagcag gttggccatg aaagggaagg cgtgatgaag
     6421 ggggagtacc gacagaaagt tgtcttggga ggtcacggcg tccaaccgca ccacgcccag
     6481 gtagttggca tagaagttct tgtgggtgag catcacgcct ttaggaaggc cggtggtgcc
     6541 ggaggtgaaa ataatggagg caaggtcctc gaggccggcc tcggggaaat ccgccgaggt
     6601 ggatacaact cggggaattt cggaaaagga cacggcatgg ggaagggatg ggtttggtcc
     6661 gcctacggag acgaacccct tgagggacga gggaaggacc gtgttcttcc aggagagccc
     6721 cgaagacgtg aaaacgaagg tcgccctggt tgtttcgaga atataggaga cgatgtcggg
     6781 tttggactgg ggatcgatgg ggacggcgac ggctccggaa aagagaattc cgaaatagct
     6841 catgggccat tcggggcggt tttcgagaat caaggcgacg cggtctcccc gggccacccc
     6901 ccgggcctgg agccaggatg cgacgcccag ggaagattcg agcagatcgc cgtacgggat
     6961 ctcgatgcgt tcttcccggt ccctgtagac aacggcaact ttccggggat cgtcctccgc
     7021 cacttgtcgc agcttccggg gcaatgtctg aaacatggca tccctcatga ttctaggctc
     7081 gcctgcgggg agcgggccca aggcgtcccg ggtggggacc gggaaaccca ccggcccttt
     7141 cattgaagag tcctttctac aatattggat gtgcattcca caacccgggc cggcggtcgg
     7201 cctttttcgg aggcggtcgg gctttggcgg tcccgggtcg ggggaaccct cgtgaagatg
     7261 atgaaatatc atcaaaatga gaacctgtgg agagattttc ccgaagatcg ccggaccgga
     7321 atcccggggc cgggtcgagg tcttccgggc catatccgat cgtccggaag acgggatgag
     7381 acccggaggg ggctttcgcg ggaagggcgg cgggactcga gacgccggcc ccgggcaggc
     7441 cgggagccga aggagggact ctcttgcgaa agggactcga gggaagtgtt cacggaacct
     7501 ccgggccaca aaaagaaaga gagccggctg ggtaagtcgc ctggctctcc gtcgcttgga
     7561 agtggggcat gtgttggtgg aatctgaccc ttctttatgc aagaatgtgg ccagatgtgg
     7621 aagacgagtc gagaaaagtc gtctcgaagg cacggcgccg ggcaaggggc gcagagctgt
     7681 cgaggaaagt tcccgttgaa accggcgaga ccttgtgtta ggaaaggcgc gagggtcgct
     7741 aacaaattcc aaggggaggg aaggatatgt caaattgggc ttggcgtgcc ggaatggtca
     7801 ttttggcaat tgttcctgcc atcatcggtg gagggctgtt cttccacctg ttcgagaact
     7861 ggacgggggt cgtggtgtgg gaaatcgttc tgctgtttct cttgagcctc gtgatttcca
     7921 agggggacaa gcgggcagcg gcccattgag gatttgaggc atggaatgca agcaggaaca
     7981 gaacctgaag aattgcaact gcagctacga tccctgcgcc cggaagggga cctgctgtga
     8041 ttgcctgcgt taccatctca aaagccgaca gctcccggga tgctgtttcc cggaaaaggc
     8101 cgaaagaacc tacgaccgct ctttcgagca tttcgcccga ctggtggccg aaaagaagat
     8161 ctgactagat tctagaaccc ttgttgcacg aggtgagcgg ttcctggagc cgctcatttt
     8221 atttggggaa acgataccac tcggggtagc ggcaacgggc ggagaaccga tggatgggcg
     8281 ggtgcccgtg gtaagggccg ggcggtcggg ggaaatcggg aatcctgctc tcgggcgctg
     8341 gagtgagcgg gtggggagcg gttccgaggt ttcccttgac ttttcacaag gcgtttctta
     8401 aggaaaagtc gcctaccggg gaggtcccgg tttcgttttt gggaattttt tcgaggggct
     8461 gccgttccgg ctggggagag atccctgcag agggagtctg ggggctttgg gggcggcact
     8521 cccgcggatt tgggtcgagg attcttcatg gaacggatac aggccgttcg aggcatgaac
     8581 gacatcttgc ctggggaaat cggctggtgg cagcaggtag aggaggcggc ccgccggatt
     8641 ttccaggatt ttggatacgt ggaaattcga actcccgtgt tggagaaact ggagctcttc
     8701 acccggagta tcggggaaaa caccgacatt gtggaaaagg agatgtatgc tttccccgat
     8761 cgaaaagggg aatggcttgc cttgaggccc gaagccacgg catccatcgt tcgctctttc
     8821 atccagaacg acctgtatgc cgatcccctg gcccggaagt tttactccat ggggcccatg
     8881 tttcgccacg aacgtcctca gaaaggacgg taccgccagt ttcaccaggt ggatgtggaa
     8941 gcctttggaa tcgacgaccc gatgctggac gcggaaatca tgttcatgct ccggcttttt
     9001 ctccaaaagg tcggactggc gggagtggga ctccacatca attccctggg ttgccgggat
     9061 tgccgaaagt cctaccggga aagccttcgg gtgtttctgg cctcccaagc ggaaggcttg
     9121 tgccccgatt gcctgcgccg ccgtgaagtg aatcccctgc gggtcctgga ctgcaaggtg
     9181 gaacgttgca agaccgtcct cgagacggcg cccgtgctgc tggagaccct ctgcgacccc
     9241 tgcagggatc atttttcccg ggtgcaggac tacctttcca ggcttgacac cccttttgcc
     9301 gtcgatcccc gcatggtccg ggggctggac tactacatgc gaaccacctt cgaggtggtt
     9361 acggaccgcc tgggggctca aagcgccgtg gggggcggcg gtcgctacaa cgggctgatc
     9421 cgggacctgg gaggcccgga cctgccgggg atcgggtttg ccgtgggcat ggaacggctg
     9481 gtacttctcc ttcaacaaca gggcgggatg ccccggagga atgcccggtt ctttgtggcc
     9541 accctgggat ccgcggccag ggaggagggg tttctgctgg tccagaggct caggagcgcc
     9601 gggatcgggg ccgagctcga ttacgaaggg cggagcctga agagtcaaat gcggcgggcg
     9661 gacaagctgg gggcccggtt tgtgttcatc ctgggggaag atgagctggg acggggggaa
     9721 atccagttgc gggacatgag ggagaagaac cagaggattg tgcctttggg cgaggcggtg
     9781 cggcacgccc ggactctgcc gacggggtga cggcggacgc attgccggac cctctcggga
     9841 ccgggcgtct tattcaggac ccgggtggat cattcccatt tgggtcacca ggaggatcac
     9901 gagtggatca gctcgaaaca gcgcgggagg attcccggtc ggcggcctcg gtggaagccg
     9961 gccgagagtt gaaaccggat tcgttggagg actgggagcg atcccattcc tgtaatgttt
    10021 tggggccggg ggacgtgggg aaagtcgtcg tcctcatggg ctgggtacac cgaagacggg
    10081 accacggggg actcatcttc gtggacttgc gggaccggga aggggtcacc caggtggtct
    10141 tcgatccgca gcacgacgtc gaggctcatg cccgggccca tgttctgcgc aatgagttcg
    10201 tgattgccgt tgaggggacg gtacggccgc gccccgaggg gatgaccaac cccaagctgg
    10261 tcacggggga gatcgaggtc gtgatttccc ggctaaagat cctgaaccct tcccggaccc
    10321 ccccctttca gatcgaagac gacctggacg tgggagaaaa cattcggctc cggtaccgct
    10381 acctggattt gaggcgcccg cgcctgtacg ggaacctgct cttccgtcac cgggccgcgc
    10441 aactgacccg caattacctg tccgaacggg ggttcctgga agtggagacc ccggttctca
    10501 ccaagagcac ccccgagggt gctcgggatt acctggtccc cagccgggtg aaccccggaa
    10561 agttctacgc cctcccccag tccccccagc tcttcaagca gcttctcatg gtggcgggat
    10621 tcgatcgata cttccagatc gtcaagtgtt ttcgagacga ggacctgcgt gcggaccgcc
    10681 agccggaatt cacccaggtg gacatggaaa tgtccttcct gcgggagcag acggtctatg
    10741 acctcatcga gggctggatc gttcggatct tcggggagct cttggagctg aatctgccga
    10801 ttccctttcc ccgcatgacg tatgcggatg ccgtggatcg atacgggacg gatcgcccgg
    10861 acctgaggtt tgggttacag ttaggcgaca tcaccgatct ggcttcggcg tcggaatttc
    10921 gggtctttcg ccaggccgtg gaacggggag gactggtcaa ggtcgtccgc ctgcccgggg
    10981 gaagcggcct ctcccgcaag gaactggacg atctcatcga cttcgtcaag gtcttcggcg
    11041 cccaggggat ggcctgggtc aagattcaac ccgacggatg gcagtcgccc gtcaccaagt
    11101 tcctgagcga ggaggttcgt acggggatcg cccgacgtct cggggccgag acgggagaca
    11161 ttctcttttt tgtggcggac cagcggaagg tggttcacga cgcgttggga aacctgaggg
    11221 tgcgcctggg gcagcaagcc gggatggtgg atccccggga ataccggttc gtgtgggtca
    11281 cccatttccc catgtttgaa tgggaccagg acgagaagag gtacacgtcg gtgcatcatc
    11341 cgttcacggc tcccctggag gaagacctgg atcttctcga gacggcgccg gaacgagtga
    11401 ggagccgggc ctacgacctg gtgctgaacg ggattgaaat cggcggaggc agcatccgaa
    11461 atcatcgccg ggacatccag gaacggattt tccgcgccct gggtttgacg gatcgggaag
    11521 tggaggagaa gttcggattt ctcttggaag cgcttcatta cggggctccc ccccacggcg
    11581 ggatcgcttt cggtctcgac cgcctcatca tgctcctcac gggttccggg tccatccggg
    11641 acgtgatcgc tttccccaag acccagaagg ccacgtgcct catgagtggg gcgccttccg
    11701 aacccgacgt gcgccagctc ctggagctgt gcgtcaaggt tgaaaaagag cggaggagtt
    11761 agtctcatgg ctcggtggaa ttccaagaag aaggtcctgg atcacgagca ttcgaggttt
    11821 tggcgccaca gtctcatgga ggtggacgag cccaacctca tgcgggagat cttcccttac
    11881 gaggaagtcc cccgcatcga tttcgaccac aaactgctcc ccatcgaccc ggccgaagac
    11941 atgttcatca ccgacaccac gttccgggac ggtcagcagg cccgaccccc gtacacggtc
    12001 aagcagatcg gcgccatctt cgacttcctg caccggctgt cggggcccaa cggggtcatc
    12061 cgccagagcg agttctttct ctacaccgac aaggaccgaa aggccctgga cctctgccga
    12121 tccaaagggt accggtaccc cgaaatcacg ggctggatcc gggcggtgga gtcggacctg
    12181 cggttggttg ccgaggcggg ccttcccgag accggcatcc tgacttccgt ttcggattat
    12241 catatcttcc tcaagctgaa ggcggaccgc aaaggggtga tggccaagta cctgagcatc
    12301 gttcgagccg ccctggacaa gggcatcgtt ccccgctgcc acttcgagga cgttacccgg
    12361 gccgacgtgt acggtttttg cgtgcccttc gccatcgagc tcatgaagct gcgggaagaa
    12421 agcggcatcg acatcaaaat gcgcctctgc gacaccctgg gttttggggt cacctacccc
    12481 ggctcggcgc tccccagaag cgtacccaag ctggtgcgcg ccttcatcga cgatgccggg
    12541 gtgcccgggc accttctgga atggcacggg cacaacgatt tccacaaggt cctggtcaac
    12601 accgccaccg cctggctcta cggctgcagc ggggccaacg gcacgctctt gggcttcggg
    12661 gagcggacgg gaaacgcccc cattgaaggg ttgatcatgg aatacatcgg gatcaagggg
    12721 agcgtgcacg gcatcgacac cacggccgtc accgacgccg tcgagtattt caaggaagag
    12781 ctgggctacc gaatcccccc caactatccc tttgtgggcg aggaattcaa cgccacaagt
    12841 gcggggatcc acgccgacgg cctggtgaag cacgaggaga tctacaacat cttcgacacc
    12901 ggcaagctcc tcaaaagacc catcagcgtc atcatcaccg acaagtccgg ggtggccggg
    12961 gtggcctact gggtcaacaa ccgcctcaaa ctcgaagggg atcgcaggct cgataaacgc
    13021 cacccggggg tgctcaagat ccacaagcgg gtgatgaagg agtacgaaca gggaaggaac
    13081 acgtccattt ccaacgagga actcgagcga tgggctcgac gttacctctc cgagtacttc
    13141 gtcagcgagt tcgaccagct caagcagaag gcttatgctt tggccgccca catggtggag
    13201 gaagtggtgg aatccgaggc cgtccggagc atggattccg ggcgccagga agccgttctc
    13261 cagaccctgc tggacctcaa ccccttcatc cagtacattt acattaccga tgcccagggg
    13321 atcaagatca cccggaacat cacccacatc gtggacaagg ccaagtacga aagcgcccgg
    13381 gtgggagaag acctctccga ccggccctgg tttatcgaac ccatgaaaaa cggccgggtc
    13441 cacctgacgg atttctatac gtcccggtac acgggggccc tgtgtatcac cgtttccgct
    13501 cccatccgga acgaagtgga ggagattgtg gggatcttgg gcatggacat gcgctttgaa
    13561 gaccttgcca agatggaagc cgacggggac gccttctaag tctcaaggag ggtcgggtcc
    13621 tttgaagacc gccagctgtt cccagggtgt ccgcgccata gggtttcagg gaaggtccgg
    13681 cctgaggttg gctgtgggat tcggcttcct tgtcctcgtt ctcctcgaaa gctgcgccaa
    13741 accgccgccc ccttctccca ccatacctcc ccccccgggc ctgcgcagcc agaagcccta
    13801 ccagatccgc gggatctggt actaccccct tcccaccgcg gaaggttacg tggaggaagg
    13861 agttgccagc tggt
//
LOCUS       DAOOVC010000102        13702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_2990_length_13702_cov_6.687624, whole genome shotgun sequence.
ACCESSION   DAOOVC010000102 DAOOVC010000000
VERSION     DAOOVC010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2990_length_13702_cov_6.687624"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLM79_12480"
     CDS             complement(<1..518)
                     /locus_tag="PLM79_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87174.1"
                     /translation="MRSRDVRHLSEDRILQSHVDGTMLTEEDRKHLSECAECRLAAAE
                     IGDSLKAFTQLAERIAPKPSRKPSILPREHAAFSRTSQWRRPFLTGFATASVLVILSG
                     IVWNQSFRTDDMAQLHEEMIQDQLLIAEIDKLEENALPTVYVEISDGQGPDLDMDGFE
                     EDKALFHGSREG"
     gene            complement(515..1264)
                     /locus_tag="PLM79_12485"
     CDS             complement(515..1264)
                     /locus_tag="PLM79_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOV87175.1"
                     /translation="MGVLAPPGMGERTAPEIADKPLGTKIGVGLLQDGQCSRAMAHST
                     APHSEASRRPEPESVAALVEQAKGGSTNAFEQLVDLYHEEIFRMVFYRTQSRMDAEDL
                     TQDIFFLMIKHIASLQEPLRFRSWLYRIAVNRVRDFHRKKRFLTLFGITGPEPGDGDQ
                     GRTEVAEEPRVLDQLIQHEFWAQVTRLSKSLSRLEREVFFLRFTEQFGIKEIAQILGK
                     SESAVKTHLYRALKKFKDNTAFSKLLQGDRA"
     gene            complement(1752..2471)
                     /locus_tag="PLM79_12490"
     CDS             complement(1752..2471)
                     /locus_tag="PLM79_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOV87176.1"
                     /translation="MPTLTLRYEQTPIKEYHLKKGQSLTIGRKSTNDVVIENLGVSGH
                     HAKIDSIDDNFLLTDLQSRNGTFVNEKLISSHWLKHGDLITIGKHTLAFAFAEGETRP
                     PAADESAMEQTMVIETDQYQSMLAKSFPQAEQQIERQTVGVLSFLAGGAGDVKLTKKL
                     TRVGKDSSCDVVVGGLMVGKVAATISQRPAGYYLSYVGGMAKPKVNGELVKESVKLEE
                     FDIIEIGSSKLQFVTDYSYKK"
     gene            complement(2502..3344)
                     /locus_tag="PLM79_12495"
     CDS             complement(2502..3344)
                     /locus_tag="PLM79_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOV87177.1"
                     /translation="MVVVESAGLTDVGRKRKANEDSLLVDDDLQLYIVADGVGGRNAG
                     EVASRIVVQTIQDYMKRFKEGGTVEELEDVDHSLSKEANRLLAGVRLANWGVNQLAGS
                     NEAYHGMGSTVAAVYIADGIFVAVNVGDSPIYLVHSRDIERISVLHTVLEEHKALDPR
                     TQARLGERFRHMLTRAMGIAETVKPDICEIQCFKDDILVLSSDGLSDLVAPEEIRDTV
                     LRERPEKACRSLVDLANERGGDDNITVVVVKVKETKQDNTGWLGRFFQAWKNLLSVFP
                     KRTQ"
     gene            complement(3463..5997)
                     /locus_tag="PLM79_12500"
     CDS             complement(3463..5997)
                     /locus_tag="PLM79_12500"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV87178.1"
                     /translation="MNILKKRPELFLGLVLTVLFVWLGLVRMEFLDALELKFYDLRMK
                     LRGEAAAPSDIVMVEIDDDSIEKLGRWPWPRSLVAEGIKKINTGSPKIIGLNLMFSEP
                     EQNVALKEIRQLEALFEKVVSPQGGDSILAFRRALQDAQARLDNDGKFAQALKEAGII
                     VLPVFFKGSIAGGEPTEEATRILSGQSLQNVRVPAGLRPPSANDIVLPLPEFLESSKG
                     LGHINIRYDLDGKARREQLVYEYRGLYVPSYTLKLAALYLNVPGEKLRAVLGTSIHLD
                     NLEVPMTVNSELLVSFKGHHGSFKKYSFFDVINDKIPAGVFKNKLVLVSTTASGIMNP
                     LSTPTDPTMSVGEFSANVIWAILNRQFVQQPVWDFTAELVLILLVGLVITFLFPRLKA
                     LTAASAFLILLFVLLGGSLHLFVSKGLWFSVTYPVVMLLLGYIGVVSLQYFVTETTKE
                     KVEGESAETNRMLGLSFQSQGMLDMAFDKFRRCPVDEGMKDILYNLALDYERKRMFNK
                     AVAIYEHIEAHDGRFKDIGDRKKKLIQASETIVLGAGGPGEPLMGTGTDVRPTLGRYE
                     IVKPLGKGAMGVVYLGQDPRIHRITAIKTFRFTEDFDPEEVKSMKEKFFIEAESAGTL
                     SHPNIVTIYDAGEEQELAYIAMEYLEGEDLQKYTREEELLPVRKVMDYAADIADALDY
                     AHQKGIVHRDIKPANIMLLKTGIVKITDFGIARITSASQTQTGVVKGTPFYMSPEQIS
                     GQKVDGRSDIFSLGVMMYQLLTGKTPFSGENLAALMHQIMNVPHPDPRQYNPKIVKPL
                     VDILNKSLEKDREKRYQRASQMASHLRQLGRKIDEMVQAKKTGSSP"
     gene            6462..7649
                     /locus_tag="PLM79_12505"
     CDS             6462..7649
                     /locus_tag="PLM79_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV87179.1"
                     /translation="MKRSGFQESGKMIRSLRISAVGWVLLLFVLSGCSKDAAGNPKGE
                     DPRKKTAIPVTVAEAEQKNVPIQLRAIGNVQAYNTVTIRAQITGELLAVHFKEGQEVK
                     KGDLLFTIDPRPFEARLKQAEALLAKERAQLANARKQVERYGSVVKRGYVPEEQFDQI
                     SANAAALEAAVRADEAAVEMARLELKYCYIRSPINGTMGEVKVHQGNLIKANDNDNPM
                     VTIRQVSPIYVSFSIPERDLPAVKKYMATGKLGVTSLVPGLESDPVQGELDFVDNTVD
                     PKTGTIQLKAVYENRDKKLWPGQFVHVVLTLATQYDAVVLPSQAVQTGQGGQYVFVVK
                     PDFTVDYRPVTAGRILDRDIVIEKGIAPGEMAVTDGHLRLAPGSKVSLVENNGKKTQE
                     SRS"
     gene            7646..10804
                     /locus_tag="PLM79_12510"
     CDS             7646..10804
                     /locus_tag="PLM79_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV87180.1"
                     /translation="MNLSEVYIHRPVMTTLVMMSILLFGIMGYRSLPVSELPNVDFPT
                     IQVSATLPGSSPETMASSIATPLEREFSTIAGLDSMTSTSALGITLITLQFAMDRNID
                     GAAQDVQTAISKAQRLLPADMPSPPSYRKVNPADQPILYLALSSPILPLSMVNEYAET
                     LMAQRISMVSGVAQVQIFGAQKYAVRVQVNPETLSKLGIGLDEVEKAISQGNVNLPTG
                     TLYGRHQAFTVQATGQLMEASAYRPLIVAYRNGSPVRLQDIGRVVDSVENDKVASWYN
                     DARTKTRAIVLAIQRQPGTNTVEVADAIKELLPTFRAQMPASIDLNVLYDRSVSIRHS
                     IDDVKFTLYLSMILVILVIFLFLRNLSATVIPSLALPMSIVGTFAVMYLLGYSLDNLS
                     LMALTLSVGFVVDDAIVMLENIVRHMEMGKGRLQAALDGSREIGFTILSMTLSLAAVF
                     IPLLFMGGILGRLLHEFAVTIGVAILVSGFVSLSLSPMLCSRFLTPPGEKGRGRLYRV
                     SERFFDGMLRWYDSSLRWVLRHRPSTMVVSLSLVFLTLYLFYAIPKDFIPSQDTGQIF
                     GFTEAAQGISFKAMVEHQLRVADVIRKDPAIESFMSAAGATGWSPTGNSGRVFIRLKP
                     RSERKDSVDRIIQRLRRELAEVPGINTFLQNPPAIQIGGRLTKSLYQYTLQAPEIDEL
                     YRWAPQLEAKLRALPGLQDVTSDLQITNPQVVVEIERDKALALGVTAEQIENTLYNAY
                     GSRQISTIYTPTNQYWVILEVQPEFQMSPAALSMLYVRSDTDRLVPISTVARLKQSVG
                     PLTVNHTGQLASVTVSFNLRPGVALGEAVNQIANAVRELRLPATITTSFQGTAQQFQS
                     SMKGLLVLLIVAILVIYIVLGILYESFIHPLTILSGLPAAGVGALLTLMIFNVDLSIY
                     AFVGIIMLVGIVKKNAIMMIDFALSAQRNDGKSPGEAIYDGCILRFRPIMMTTMAALM
                     GTLPIALGFGAGAEARRPLGLAVVGGLLLSQFLTLYITPVVYLYMESVQTWVARRVKT
                     GRGSGDRAGPKKESPVAVDEFP"
     gene            10881..>13702
                     /locus_tag="PLM79_12515"
     CDS             10881..>13702
                     /locus_tag="PLM79_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOV87181.1"
                     /translation="MNIPELFIRRPVTTTLVMMSILLFGIMGYRSLPVSELPNVDFPT
                     ILVSANLPGSSPETMASSVATPLEREFSTIAGLDSMTSTSALGVTQITLQFSLDRDID
                     GAAQDVQTAISKAQTLLPSDMPAPPSYRKVNPADQAILYLAVSSRILPLSTVHEYAET
                     LMAQRISMVSGVAQVQIFGAQKYAVRVRVNPETLSKLGIGLDEVEKAIVRANVNLPTG
                     TLYGTHQAFTVQATGQLMEAAAYRPLIVAYRNGSPVRIEDIGQVTDGVENDKIASWFV
                     DQGLPVRAIVLAIQRQPGTNTVEVANSIKGLLPMFRAQMPASLDLNILYDRSTSIHDS
                     IEDVKLTMYLSILLVVLVIFLFLRNLSATTIPSLALPMSIVGTFAVMYLLGYSLDNLS
                     LMALTLSVGFVVDDAIVMLENIVRHMEMGKGRLQAALDGSREIGFTILSMTLSLAAVF
                     IPLLFMGGILGRLLHEFAVTIGAAILVSGFVSLSLSPMLCSRFLAAPGEERRGRIYVA
                     SERFFQGMLHCYDASLRWVLRHRGWTMALSVGLLIVTVYLFVVVPKDFVPSQDTGQIF
                     GFTEAAQGISFEAMVERQMEVVNVVLQDPAVEAFMSTVGATGASPTGNTGRLSVRLKP
                     REERDANADEVIQRLRRKLASVFGINTFLQNPPAIRIGGRLTKSLYQYTLQSPEIGEL
                     YTWAPQLEARLRTLPGLQDVTSDLQITNPQVVVEIERDKALALGVTAEQIENTLYNAY
                     GSRQISTIYTPTNQYRVILEVQPEFQMSPAALSMLYVRSNTGRLVPISTVARLTQSVG
                     PLTVNHTGQLASVTVSFNLKPGVALGEAVKQIERAVRDLRLPASITTSFQGTAQQFQS
                     SMKGLWVLLIVAILVIYIVLGILYESFIHPLTILSGLPAAGVGALLTLMIFRVDLSIY
                     AFVGIIMLIGIVKKNAIMMIDFALSA"
BASE COUNT         2646 a         4138 c         3947 g         2971 t
ORIGIN      
        1 atgccttccc tcgacccgtg gaaaagcgct ttgtcttcct cgaagccgtc catgtccaaa
       61 tcggggcctt gcccgtccga tatctcgaca tagacggtgg gcaaggcgtt ttcttcgagc
      121 ttgtcgattt cggcaatgag aagctgatcc tggatcatct cctcgtgtag ttgagccatg
      181 tcgtccgtcc gaaagctctg attccagacg atcccggaaa ggatgaccag gacggatgcg
      241 gtggcaaacc cggtgagaaa cgggcgccgc cattgggatg tgcgggagaa tgccgcgtgt
      301 tccctgggaa ggatcgaagg tttccgggac ggtttcggcg cgatcctctc cgccaactgc
      361 gtgaaagcct tcagggagtc cccgatctcg gctgcagcaa gccggcattc cgcacactcc
      421 gaaagatgct tccggtcttc ctccgtcagc atcgtcccat cgacgtggga ctgcaagatc
      481 cggtcttcgc tcaggtgtcg aacgtcacgg gacctcatgc tctgtctccc tgcaaaagct
      541 tcgaaaaagc cgtgttgtcc ttaaacttct tcaaggctcg gtatagatgc gtcttgacgg
      601 cactctcgct cttccccagg atttgcgcaa tttccttaat gccgaattgc tccgtaaatc
      661 tcaggaagaa gacctctcgt tccagcctcg acaaagactt ggaaagtctg gtcacttgtg
      721 cccagaactc gtgctggatg agctgatcaa ggaccctggg ttcctcggcg acttccgtgc
      781 ggccctggtc cccgtctccc ggctcgggac cggtgatccc aaagagcgtc aggaagcgct
      841 tcttgcggtg gaagtcccgg acccgattta cggcgattcg atagagccat gagcgaaagc
      901 gcaagggttc ctgaagggat gcaatatgtt tgatcatgag aaagaatatg tcttgggtca
      961 ggtcttcggc atccatgcgc gactgagttc gatagaagac catgcgaaag atctcctcat
     1021 gatacaaatc caccagctgc tcgaaggcgt ttgtgctgcc ccctttggcc tgttccacca
     1081 gtgctgcgac ggattccggt tccggcctcc gggaagcttc gctatgggga gcggtcgagt
     1141 gcgccatggc cctgctgcat tgtccgtcct gcaagagccc cactccgatt ttcgttccca
     1201 agggcttgtc ggcgatttcc ggagcggtcc tctcgcccat gccaggaggc gccaagacac
     1261 ccattcagcc tcatccccgc ctggcgactg ttcccattac tccaccctcc gttttcattc
     1321 tcatctgtca ccggaattta ctctatatag acgacggatg gtatccgttt gtctacgatg
     1381 gggaccccaa aaaagcgtga ggtgaagatt ttcggacggt gcgagggatt tccgcgaaga
     1441 aggcagagaa accgccggcc gttccctttg tgctgcgcca aaggaccttt ttcaagcccc
     1501 ggaggatgag cccttgtcgc gaaacgacaa aaagaaggat ggaatctgaa ttgagcgatc
     1561 cgctgtcagc tccatggtat ctcaaacttt tcggaagttc cccgttttgg ggggagccgg
     1621 agcggttcga ccacaggaaa tttcagagct gacggcgaag cgctaaccgc tcgatttgga
     1681 tagaaacgac ccgcgaccgg ccccgctgcg gggtcgttga ccctgttggc ccggtctggt
     1741 ggccgtgggc gctatttctt atacgagtag tcggtgacaa attgaagctt gctggacccg
     1801 atctcgatga tgtcgaactc ctcgagcttg accgactcct tgaccaactc cccgttcacc
     1861 ttcggttttg ccattccacc cacatagctc agataatagc ctgcaggtcg ctggctaatg
     1921 gtggcggcga ctttgcccac catgagcccc ccgacgacca cgtcgcagga gctgtccttc
     1981 ccgacccggg tgagcttctt ggtcagcttc acgtctccgg cccctccggc caggaaagag
     2041 agcaccccga cggtttgcct ttcgatttgc tgctccgcct gggggaaact ctttgcaagc
     2101 atcgactggt actgatcggt ttcaatgacc atggtctgtt ccatggcgct ctcatcggcg
     2161 gccggggggc gggtctctcc ctcggcaaag gcgaaagcca gggtgtgttt gccgatggtg
     2221 atgaggtcgc cgtgtttcag ccagtgggaa gagatcagct tctcgttgac gaaggtaccg
     2281 ttccgactct gaagatcggt caggaggaaa ttgtcatcga tggagtcgat cttggcatgg
     2341 tgcccggaca cccccaggtt ttcgatgacc acatcgttgg tggatttccg accgatggtc
     2401 agggattgcc ccttcttgag atggtactct ttgatcgggg tctgctcgta tcgaagagtc
     2461 aacgttggca tcgcaaagct ctcttttctg gatcggttgg atcactgggt ccttttgggg
     2521 aaaaccgaca aaagattctt ccaggcctgg aagaatcgac ctagccaccc ggtattgtct
     2581 tgtttggttt ccttcacctt gaccaccacg acggtaatgt tgtcgtcgcc tccgcgctcg
     2641 tttgccaggt ccaccaggga ccgacaggcc ttttccgggc gctcccgcaa gaccgtatcc
     2701 cggatctctt cgggagccac caggtcgctc aacccgtcgg aactgaggac caggatgtcg
     2761 tctttgaaac actgaatctc acagatgtcc ggcttcacgg tttcggcaat ccccatggcc
     2821 cgggtgagca tatggcggaa cctctcgccc agacgtgcct gggtcctggg gtccaatgcc
     2881 ttgtgctcct ccaggaccgt gtgcagcacg gagatcctct cgatgtcccg ggaatgaacc
     2941 aggtagatgg ggctgtcccc tacgttgacg gcgacgaaga tcccgtcggc aatatagacg
     3001 gccgccaccg tcgagcccat cccgtggtaa gcttcattgc ttccggccag ctgattgact
     3061 ccccagttgg ccagacggac accggcaaga aggcggttgg cctctttgga gagactgtga
     3121 tccacatcct ccagttcctc gacagttccg ccctctttga accgcttcat gtagtcctgg
     3181 atggtctgga ccacgattcg gctggccact tctccggcat tgcggccccc gaccccatcc
     3241 gcgacgatgt agagctgaag atcgtcgtcg accagcaaag aatcttcgtt ggcctttctc
     3301 tttctcccta cgtccgtcag tcctgccgat tcgacgacca ccatagcgcg aaactctagc
     3361 tatattcagg aggttggttg ccagtcattc ccatgggtcc catagtataa agatttttca
     3421 tcctttacaa gctcatatac aggtccatgg cgcggcgaat tcctatgggg atgaaccggt
     3481 cttcttggcc tggaccatct cgtcgatctt tctgcccaat tgtcgcagat ggctcgccat
     3541 ctgggacgcc cgctggtagc gcttttcccg gtctttctcc agggacttgt tgaggatgtc
     3601 caccaggggc ttcacaatct tggggttgta ctgccggggg tccggatggg gcacgttcat
     3661 gatctggtgc atgagggcgg caaggttctc tcccgaaaag ggggtcttgc ccgtgaggag
     3721 ctggtacatc atgactccca gggagaagat gtccgacctc ccgtccacct tctgccccga
     3781 aatctgctcg ggggacatat agaacggagt gcccttgacg acccccgtct gggtctgcga
     3841 ggcggaagtg atgcgggcga tgccgaaatc cgtgatcttt acgatcccgg ttttcaggag
     3901 catgatattg gcgggcttga tgtcccggtg cacgatgccc ttttggtggg cgtaatccag
     3961 agcgtcggca atatcggccg cataatccat gactttgcga accggaagca gctcttcctc
     4021 ccgggtgtat ttctggagat cttctccctc caggtactcc atggcgatgt aggccagctc
     4081 ctgctcttcc cctgcgtcgt aaatggtcac gatgttggga tgggacaagg tccccgcact
     4141 ttcggcctcg ataaagaact tctctttcat gctcttgact tcttcgggat cgaagtcttc
     4201 ggtgaatcga aaggtcttga tggcggtgat ccggtgaata cgcgggtcct gccccaggta
     4261 gaccactccc atggctcctt tccccagggg cttcacaatt tcatatctgc ccagggtggg
     4321 acgcacgtcc gttcccgtgc ccatgagggg ctcgccgggt ccgcccgctc ccagaacaat
     4381 ggtctcactg gcctgaatga gcttcttctt ccggtctccg atatccttga acctgccgtc
     4441 atgggcttcg atatgctcgt aaatcgccac ggccttgttg aacatcctct tgcgctcata
     4501 gtccagggcc aggttgtaga ggatgtcctt cattccctca tccacggggc accggcggaa
     4561 tttgtcgaag gccatgtcca gcatcccctg actctgaaaa gacagcccca gcatgcggtt
     4621 ggtttccgcc gattctcctt cgactttctc tttggtggtt tcggtcacga aatattgcag
     4681 actgaccacc ccgatgtatc ccaggaggag catgaccacg ggataagtga cgctgaacca
     4741 gagccccttg gaaacgaaga gatggaggga cccgcccagg agcacgaaaa gcaggatcag
     4801 gaacgcggaa gccgccgtca gtgctttgag tcgggggaac aggaaggtga tcaccaggcc
     4861 cacaaggagg atgagcacaa gctccgccgt gaagtcccac acgggttgct gcacgaattg
     4921 ccggttgaga atggcccaga tcacgttggc cgagaattcc cccaccgaca tggtggggtc
     4981 cgttggggtg ctcaagggat tcatgatgcc cgaggccgtc gtgctcacca gcaccagctt
     5041 gttcttgaac acgccggcgg ggattttgtc gttgatcacg tcaaagaagg agtatttctt
     5101 gaatgagccg tggtgtccct tgaagctcac caggagctcc gaattcacgg tcatggggac
     5161 ctccaggttg tccaggtgaa tggaggtccc cagaacggcc ctcaattttt ccccgggcac
     5221 attcaggtaa agcgccgcca gcttgagggt gtaggaggga acgtaaagcc cccgatactc
     5281 gtacaccagc tgctcccttc tcgccttacc gtccaagtcg tagcgaatgt tgatgtgacc
     5341 cagtccctta ctgctctcga ggaactcggg caagggcagc acgatgtcgt tggccgaagg
     5401 cggacggagt ccggcaggaa cccgcacgtt ctgaagggat tgcccggaga gaatccgggt
     5461 tgcctcctcg gtcggctccc ctccggcgat gcttcccttg aagaaaaccg gcaaaacaat
     5521 gatcccggcc tctttgagtg cctgggcaaa cttgccgtcg ttatcgaggc gcgcttgggc
     5581 gtcctggagg gcccggcgga atgcaaggat cgagtctccg ccctggggcg aaaccacttt
     5641 ctcgaagagg gcttcaagtt gtcggatctc tttgagggcg acgttctgct ccggttcgct
     5701 gaacatcaga ttgagtccga tgatcttggg ggaccccgtg ttgatcttct tgatcccctc
     5761 cgccacgagg gagcgcggcc aaggccagcg tcccagcttt tcgatggaat cgtcgtcgat
     5821 ttccaccatg acgatatcgc tgggggccgc agcctctccc cggagcttca tgcgaaggtc
     5881 atagaacttg agctcgaggg catccaggaa ctccatgcga accaggccca gccagacgaa
     5941 aaggaccgtc aacaccaaac caaggaatag ctccgggcgt ttcttgagga tgttcattct
     6001 gggtccacct gccgtcgaac ggttcgagaa cgggatcggc ttctttgccg ctgagaagat
     6061 ttcaaccctt ttgtgtggtc tacagtctcg ctgaacggag gtttcatgtc aatgcaaaag
     6121 ctatggaatt ccctacgtat tccaggggat gaaaagattt tgtgcctgaa tatcggatgg
     6181 accgtcgagg ggaggagagg gcctttccct ttcggccggg ggcccgattg tgccgaccgc
     6241 tttcgggcga agacaagaag ccgtgattcg gcttcttccg ggcagaccgt ccaggggggg
     6301 gacccgaccc gggttttgct tttccccctt cgacggtttc ggtataattc gtcggtcata
     6361 ggacggttat tcgcccgcat tgaagaaaca aattgcacaa gccgttcccg ggcggctccc
     6421 atgcacagaa acatgctggt ggcgggaaga aggtttttgg aatgaaacga tccggtttcc
     6481 aggaaagtgg gaagatgatt cgatccctga gaatttcggc agtgggatgg gtgctcttac
     6541 tttttgtcct ttcgggttgt tccaaagacg ccgccggaaa ccccaagggg gaggaccccc
     6601 ggaagaagac ggcaatcccc gtgacggtgg cggaggctga gcagaagaac gtccccattc
     6661 agttgcgtgc catcgggaat gttcaggcgt acaacaccgt gacgatccgg gcacagatca
     6721 cgggtgaact gctcgcggta catttcaagg aagggcagga agtcaagaag ggggatctgc
     6781 tgttcaccat cgatcccagg cccttcgagg cccggctcaa acaggcggaa gccctgctgg
     6841 ccaaagagcg ggcccaactg gcgaacgccc gaaagcaggt tgaacggtac ggttcggtgg
     6901 tgaaaagggg ctatgtgccc gaagaacaat tcgatcaaat cagcgccaac gccgcggccc
     6961 tcgaggccgc cgttcgggcc gacgaggccg cggtggaaat ggccaggctc gagctcaaat
     7021 attgctacat ccggtctccc atcaacggaa ccatgggaga agtcaaggtg caccagggaa
     7081 acctcatcaa ggccaacgac aacgacaatc ccatggtgac catcaggcag gtgagtccca
     7141 tctatgtcag tttctcgatc ccggaaagag acctcccggc ggtgaaaaag tacatggcga
     7201 cgggaaagct gggggtgacc agcctggtcc ccggcctgga aagcgatccc gtccaggggg
     7261 agctcgattt tgtggacaac accgtcgacc cgaaaaccgg gaccattcag ttgaaagccg
     7321 tttatgagaa ccgggacaag aagctgtggc ccggccagtt tgtgcacgtc gtcctgaccc
     7381 tggccaccca gtacgacgcg gtagttctgc cgtcccaggc cgttcagacg ggccagggcg
     7441 gtcaatacgt cttcgtggtc aaaccggact tcaccgtgga ctaccgtccc gtgacggccg
     7501 gcaggattct ggatcgggac attgtcattg agaaggggat tgcaccgggt gaaatggctg
     7561 tgaccgacgg ccatttgcgg ctggccccgg gatccaaggt gagcctggtg gaaaacaacg
     7621 ggaagaagac ccaggaatcc cgttcatgaa cctgtcggag gtttacattc accgccccgt
     7681 catgaccacc ctggtcatga tgagcatcct tcttttcgga atcatggggt accggtccct
     7741 gccggtaagt gaactcccca acgtggattt ccccaccatc caggtgtccg cgaccctgcc
     7801 cggatccagt ccggagacca tggcatcctc cattgcaacc cccctcgagc gcgaattttc
     7861 caccattgcc gggctggatt ccatgacgtc caccagtgcc ctgggcatta ccctcatcac
     7921 cctccagttt gccatggacc ggaatatcga cggggcggcc caagatgtcc agacagccat
     7981 atccaaggcc caacggctcc tgcccgcgga catgcccagt cctccgtcct accggaaggt
     8041 gaacccggcg gaccagccca ttctttacct ggccttgagt tctcccatct tgccgctttc
     8101 catggtcaac gagtacgccg aaaccttgat ggcccagcgc atctccatgg tgagcggagt
     8161 ggctcaggtc cagatcttcg gcgctcagaa gtatgcggtg cgggttcagg tgaatcccga
     8221 aaccctgagc aaactgggca tcgggctgga cgaggtggaa aaggctatct cccagggaaa
     8281 cgtgaacttg cccacgggga ccttgtacgg gaggcaccag gctttcaccg tccaggccac
     8341 cggccagttg atggaagcct ccgcctatcg gcccctcatc gtcgcctatc gaaacgggtc
     8401 ccccgtccgg ctccaggaca tcggccgggt ggtcgacagt gtggaaaacg acaaggtggc
     8461 cagttggtac aacgatgccc ggaccaagac ccgggccatt gtcttggcca tccagcggca
     8521 gccggggacc aataccgttg aggtggcgga cgccatcaag gagcttctgc ccacctttcg
     8581 agcccagatg cccgcatcca tcgacctgaa cgtcctctat gaccggtcgg tgtccattcg
     8641 acactccatc gacgacgtga aattcactct gtacctgagc atgatcctgg tcatcctggt
     8701 catctttctt ttcctgcgca acctgtccgc cacggttatc cccagcctgg cccttcccat
     8761 gtccattgtg gggaccttcg ccgtgatgta cctgctggga tacagcctgg acaacctgtc
     8821 cctgatggcc ctgaccctca gcgtggggtt cgtggtggac gacgccatcg tcatgctcga
     8881 aaacatcgtg cgccacatgg agatggggaa ggggaggctc caggcggccc tggacgggtc
     8941 ccgggagatc gggttcacca tcttgtccat gaccttgtcc ctggcggcgg tgttcatccc
     9001 cctgctgttc atgggaggaa tcctgggtcg cctgctccac gaatttgccg tgaccattgg
     9061 agtcgccatc ctggtttcgg ggttcgtctc cctgtccctg tctcccatgc tctgcagccg
     9121 gttcctgact ccccccgggg agaaggggcg ggggcgcctg taccgggttt cggagcggtt
     9181 tttcgacgga atgcttcgct ggtacgactc gagtctccga tgggtgttgc gtcaccggcc
     9241 ctccacgatg gtggtttccc tctccctggt tttcctcacc ctctacctgt tctacgccat
     9301 tcccaaggac ttcatcccca gccaggacac ggggcaaatc ttcgggttta ccgaggcggc
     9361 ccaggggatt tccttcaagg ccatggtcga gcaccagctt cgggttgcgg acgtcatccg
     9421 aaaggacccg gccatcgaga gcttcatgtc ggcggcgggg gccacggggt ggagtcctac
     9481 gggaaattcc ggaagagtgt tcatacggct caaaccccgg tccgaacgca aagacagcgt
     9541 ggaccggatc atccagcgcc ttcggcggga actggccgag gtccccggaa tcaacacctt
     9601 tcttcaaaat cccccggcca tccagatcgg cggccggttg accaagagcc tctaccagta
     9661 caccctccag gcgcccgaga tcgacgagct ctaccggtgg gccccccagc tggaggcgaa
     9721 gctccgggcg ctgcccggac tccaggacgt gaccagcgac ctccagatca ccaatcccca
     9781 ggtggtggtg gaaatcgagc gggacaaggc cctggccctg ggagtcacgg cggagcagat
     9841 cgaaaatacc ctctataatg cctacggttc ccgccagatc tccaccatct acacccccac
     9901 caaccagtac tgggtgatcc tggaagtgca gccggaattc cagatgtctc ccgccgccct
     9961 ctccatgctt tacgtgcggt cggatacgga ccggctggtt cccatctcca ccgtggcccg
    10021 gctcaaacag agcgtgggtc ccctcacggt gaaccatacc ggtcagctgg cttcggtgac
    10081 cgtgtcgttc aacctcaggc ccggcgtggc cctcggggag gccgtgaacc agatcgcaaa
    10141 cgccgtaagg gagcttcggc ttccggcgac catcaccacg agcttccagg ggacggccca
    10201 gcagttccag tcctccatga aagggttgct ggtccttctc atcgtggcaa tcctggtaat
    10261 ctacatcgtc cttggaatcc tgtacgagag cttcatccat cccctcacca tcctttcggg
    10321 tcttccggcc gcgggagtgg gcgccctgtt gaccttgatg atcttcaacg tggatttgag
    10381 catctatgcc tttgtgggca tcatcatgct cgtgggaatc gtaaagaaaa acgccatcat
    10441 gatgatcgat tttgccttga gtgcccagag aaacgacggg aagagtccgg gagaggccat
    10501 ctacgacggg tgcatcctcc ggtttcgccc catcatgatg accaccatgg cggcgctcat
    10561 gggaaccctg cccatcgccc tggggttcgg ggccggggcc gaggctcggc ggcccctggg
    10621 gctggcggtg gtcggaggat tgctcctttc ccagttcctc accctgtaca tcacccccgt
    10681 ggtgtacctg tatatggaat cggtccagac atgggttgcc cggcgggtca agaccggcag
    10741 ggggtccgga gaccgggcgg gtcccaaaaa ggaatctccg gttgccgtgg atgaattccc
    10801 gtagcccctc gatcgaggga ggtttcccga gggttgtgtt gctgttcctt gactccatct
    10861 gaagcgagag tgaaccctgc atgaacattc cggagctctt tatccgccga cccgttacca
    10921 ccaccctggt catgatgagc atccttcttt tcgggatcat ggggtaccgg tctttgccgg
    10981 taagtgagct ccccaacgtg gactttccca ccattctggt gagtgccaac ctcccggggt
    11041 ccagcccgga aaccatggcc tcctcagtgg cgacgccttt ggagcgcgag ttctccacca
    11101 tcgcgggtct ggattccatg acttccacca gcgccctggg ggtcacccag atcaccctcc
    11161 agttttccct ggaccgggac atcgacgggg cggcccagga tgtccagacg gccatctcca
    11221 aggcccagac cctgctgcct tcggacatgc ccgcccctcc ctcctatcga aaggtaaacc
    11281 cggcagacca ggcgatcctc tacctggcgg ttagttcccg gatcctcccc ttgtccacgg
    11341 tgcacgaata cgccgaaacc ctcatggccc agcgcatctc catggtgagc ggggttgccc
    11401 aggtccagat ttttggggca caaaaatacg ccgtgcgggt tcgcgtgaac cccgaaaccc
    11461 tgagcaagct gggaatcggc ctggacgaag tggagaaagc catcgtccgg gcgaatgtga
    11521 acctgcccac gggaaccctg tacgggaccc accaggcttt caccgtgcag gccacggggc
    11581 agttgatgga agctgccgcc tatcggcccc tcatcgttgc ctatcggaac ggttcccccg
    11641 ttcgcatcga agacatcggt caggtgacgg acggggtcga gaacgacaag atcgccagct
    11701 ggttcgtgga ccagggactt cccgtccggg ccatagtgct ggccatccag cgtcagccgg
    11761 gcaccaacac cgtcgaggtg gccaactcca tcaagggcct tcttcccatg tttcgtgccc
    11821 agatgcccgc ctctctggat ctgaacattc tctacgaccg ctccacctcg attcatgatt
    11881 ccatcgaaga cgtgaagctg accatgtatc tgagcatcct cctggtggtg ctggtgattt
    11941 tcctgtttct tcgcaacctc tcggcaacca ccattcccag tctggccctt cccatgtcca
    12001 ttgtggggac cttcgccgtg atgtacctgc tgggatacag cctggacaac ctgtccctga
    12061 tggccctgac cctcagcgtg gggttcgtgg tggacgacgc catcgtcatg ctcgaaaaca
    12121 tcgtgcgcca catggagatg gggaagggga ggctccaggc ggccctggac gggtcccggg
    12181 agatcgggtt caccatcttg tccatgactt tgtccctggc ggcggtgttc atccctcttc
    12241 tcttcatggg ggggatcttg gggcggctgc tccatgaatt cgccgtgacc atcggagcgg
    12301 ccatcctggt ttccggtttc gtttccctgt ctctttctcc catgctttgc agcagattcc
    12361 tggccgctcc gggagaagaa cggcgcggtc ggatctacgt cgcttcggaa cggttcttcc
    12421 agggaatgct ccactgctac gatgccagtc ttcggtgggt cttacggcat cgagggtgga
    12481 ccatggcgct ttccgtcggg ttgctgatcg tgaccgtgta tcttttcgtg gtggtcccca
    12541 aggacttcgt tcccagccag gacacgggac agattttcgg tttcaccgag gcggcccagg
    12601 ggatttcttt cgaggccatg gtggaacggc agatggaagt ggtgaacgtg gtcctgcagg
    12661 acccggcggt ggaagccttc atgtccacgg tgggggctac cggcgccagc cccacgggaa
    12721 acacgggccg cctctccgtt cgactcaagc cccgggagga aagggacgcc aacgccgacg
    12781 aggtgattca gaggctgcgg cgaaagttgg cttcggtttt cggaatcaat acttttctgc
    12841 agaacccgcc cgccatccgg atcgggggcc gactcaccaa gagcctttac cagtacacgc
    12901 ttcaatcgcc cgaaattggg gaactgtata cctgggctcc gcaattggaa gcccggctgc
    12961 gcactctgcc cgggcttcag gacgtgacca gcgaccttca gatcaccaac ccccaggtgg
    13021 tggtggaaat cgagcgggac aaggccctgg ccctgggagt caccgcggaa cagatcgaaa
    13081 acaccctgta caatgcctac ggttcccgcc agatctccac catctacacc cccaccaacc
    13141 agtaccgggt gatcctcgag gtgcagccgg aattccaaat gtctccggcc gccctgtcca
    13201 tgctctatgt gcgatccaat acgggtcgcc tggttcccat ctccaccgtg gcccggctga
    13261 cccagagtgt ggggcccctc accgtgaacc acaccggtca gctggcttcc gtgaccgtat
    13321 cgttcaacct caagcccggg gtggccctcg gggaagcggt gaagcaaatc gaacgggccg
    13381 tccgggacct tcggcttccg gcgagcatca ccacgagctt ccaggggacg gcccagcagt
    13441 tccaatcttc catgaagggc ctgtgggtcc ttctcatcgt ggcgattcta gtcatttaca
    13501 ttgtgcttgg gattctgtat gagagcttca ttcacccgtt gaccatcctt tcgggtctgc
    13561 ctgcggcggg ggttggcgca ctgttgacct tgatgatctt tcgggtggat ttgagcatct
    13621 acgccttcgt gggcatcatc atgctcatcg ggatcgtgaa gaagaatgcc atcatgatga
    13681 tcgattttgc cctgagcgcc ca
//
LOCUS       DAOOVC010000103        13664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3009_length_13664_cov_6.866265, whole genome shotgun sequence.
ACCESSION   DAOOVC010000103 DAOOVC010000000
VERSION     DAOOVC010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3009_length_13664_cov_6.866265"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PLM79_12520"
     CDS             complement(<1..753)
                     /locus_tag="PLM79_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOV87182.1"
                     /translation="METAKNRLEDSDLSELSRCRARVRNLKECFRVSGLINSTLELEE
                     VLEHIMTASREVLGADACSLLLVDESTEELVFEVAQGPVAHKLKTGLRLKKGEGIAGH
                     VFQTGEPLIIVDAYRDERFHPDFDRKTGYRTQSILCVPLKIKDRVIGVSQVINKVDGT
                     AFLPEDAELLSLLCAHAAVAVENARIHRALLRKQQIESDLAFATSIQRSFLPPGVPQL
                     EGYRFHALYRSALEVGGDFYDFIPLEGQRLGIL"
     gene            1095..1940
                     /locus_tag="PLM79_12525"
     CDS             1095..1940
                     /locus_tag="PLM79_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOV87183.1"
                     /translation="MLRFLLVLLTGVFLIPVPCMHANAADPPTPKVKDLILATTTSTV
                     DSGLLDVLIPVFEKKTGHRVKTIAVGTGQALAMGEKGEADVLLTHAPEAEKKLVDSGT
                     VLNYLLVMHNDFIVVGPPEDTAKIKGKSSVDAFKAISQAQAVFISRGDDSGTHKRELS
                     VWKKAEVSLTGAKWYQQSGQGMGATLLMASEKQGYTLTDRATYLSQKKNLKLDILSEG
                     DKALLNIYHVMQVNPEKFPKVNGEGAKAFAEFMVAPETQKMIGEFGKDKFGQSLFFPD
                     AGKTL"
     gene            2379..2624
                     /locus_tag="PLM79_12530"
     CDS             2379..2624
                     /locus_tag="PLM79_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87184.1"
                     /translation="MKMFVWRHSKKLSSWSMFDEPHIYRDNYLRAEVSVLADSLEEAL
                     QWLESSGEWNIEELRRIEPRVVAADQPGIVWKSIDFG"
     gene            2661..2819
                     /locus_tag="PLM79_12535"
     CDS             2661..2819
                     /locus_tag="PLM79_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87185.1"
                     /translation="MDVMSSPEVVRSMPGGIPGSELAVIPRAGHGSNLENFDEFTRKK
                     TEFPEKLE"
     gene            3113..4573
                     /locus_tag="PLM79_12540"
     CDS             3113..4573
                     /locus_tag="PLM79_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HOV87186.1"
                     /translation="MKKRPGFQDWSRVRSFVFFLCLTAALLPAPATRAADGQRPSMAP
                     ENPAFQQYIEDVEFGRLEPLMVGDFPSGIVPPPVKFPRKAIESVEPMRAIPSAYDLRK
                     KGRVTSVKDQGSCGSCWAFGTYGSLESRLMPDKAWNFSEENLIENHGFDKGPCDGGNY
                     EMSTAYLARWSGPIKESDDPYNHGGGVGVSAAYKPVKHVADIVFLPERATPKDNTAVK
                     QTIMKYGAVAGYIYYSNSYYDSTNYTYYYGGSSSVNHGVAIVGWDDSFSRSKFKAYGG
                     KLPAGNGAFIVKNSYGTTYGQKGYFYVSYYDTSIEPSMAFNLVDSPTKYNQIFQYDPL
                     GAITGVGYSSTTAWGANIFKSSTTFTLKAVGFHTLVPGTSYTAYVYKGVTAGQPRSGT
                     QVAKVSGKFPMAGYHTVPIGSVGIQSGQRFSVVVCFNTPSYTYPVPVEARIAGYSSKA
                     TSTTGQGFISSAGSTWQDIYNSFANSSVCIKAMGVK"
     gene            complement(4608..5360)
                     /locus_tag="PLM79_12545"
     CDS             complement(4608..5360)
                     /locus_tag="PLM79_12545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOV87187.1"
                     /translation="MKTWDSKKRGPAKEGLGDGISYSVREDPRARRVILKISPEKGLE
                     VVVPRGFDGRRIPGILIEKKDWIHETLQRFSGRNDSGQEPFALPDSIWLRAVDERYVV
                     EPAGDDEPGFQLVLNGKVDWRVRGEVRDARHLGRVMRPWLKRVGTAHLAPWLGRIGRE
                     TGLGFRTLQVRGQKSRWGSCSSRGTISLNYKLLFLPPELVRYILIHELCHTVHPNHSP
                     RYWNLVARFEPAYRDLDRAMRTARAHVPGWAG"
     gene            complement(5341..5994)
                     /locus_tag="PLM79_12550"
     CDS             complement(5341..5994)
                     /locus_tag="PLM79_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOV87188.1"
                     /translation="MERPIDHFWQIRLNQVKKTLEEHNFEAFVADNVEEARRLLIEEI
                     VPKTGAKSVSWGGSLTFIGTGLYEKMKAVPGLEVVDTYDKSIAPEAMFERRRQALLAD
                     LFITGTNAVTESGELVNLDMIGNRVSAIVFGPRNVVILVGRNKIVADLNDAMIRVKLY
                     AAPVNAMRLDKKVPCVKTGECEDCKSPDRICNAWSIVEKSFPKGRIKVILINEDLGF"
     gene            6291..7064
                     /locus_tag="PLM79_12555"
     CDS             6291..7064
                     /locus_tag="PLM79_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOV87189.1"
                     /translation="MARFRPVFIEGRDLDDTWFQLLAQLYEHGRRYEITAGSYEGSQR
                     VAYDFVSGFIHYPHSRPLAPRVPENSPLPPPTTDEEIDKYFVNYLMCSDAAVHEDYRY
                     ATWVVGGDYELPRFQKPGLAPVDKETVRVPNQLEWIVRHFREKGHGNEHCFLTVGYPE
                     SNFAYDLPYSNPNERKTSPCLRGLDFRIVEGQLLTHVIYRSWDLASGWATNMGGFTLL
                     NEYMAAEIGVEPGPLSFSCKSLHCYEHAFEYLKLRLGKA"
     gene            complement(7126..7290)
                     /locus_tag="PLM79_12560"
     CDS             complement(7126..7290)
                     /locus_tag="PLM79_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87190.1"
                     /translation="MIMYVKDKKSNKWHWCENCSNYPTSYNVETRSTKPNSDLCDECK
                     AKEKAGECRQ"
     gene            7478..8287
                     /locus_tag="PLM79_12565"
     CDS             7478..8287
                     /locus_tag="PLM79_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOV87191.1"
                     /translation="MNNPVGADYVLLDSPAILSRLFYPREDWRPGDWEPPGEEVAIPV
                     EEGVEVGGRFHLSDPHGCNLLFFHGNGEIVADYDDIAPIFGRMGINFLPVDYRGYGRS
                     GGSPSASSMMRDSHEIFRYVRKWLDENKHSGPLMVMGRSLGSASALELAFHYPEQLDA
                     VIVESGFAYTMALLRFLGVDVEGMGLGEADGFGNIEKIRSFGKPTLLIHAELDSIIPF
                     SDAQALFDACGARDKTLVKIEGADHNNLFLVGLPTYLSSLKTLADKVKAGT"
     gene            complement(8300..9298)
                     /locus_tag="PLM79_12570"
     CDS             complement(8300..9298)
                     /locus_tag="PLM79_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOV87192.1"
                     /translation="MSVSSCMNAVVFDEGSIRLVEVPIPEPLPGEVLIRTLLTGICRT
                     DRELFEGYYGFRGVPGHEFVGRVERAPGRKEIEGSRVVADINIGCGECSWCRADVPKH
                     CLSRRAVGIQGWDGAFAQYLKVPEANVHLVEAGIRDEEAVFAEPLAAALDVSQQVHIR
                     SSDRVLVMGDGKLGLLIALCLRTLTPHLVLLGRHSEKLETARKQGIEVFNVSTPQDDP
                     SAPSPPGSFDVVVEATGSDLGIQEALAFLRPRGTLVLKTTSRVPSSVNSARIVVDEIK
                     IAGSRCGDLRLALSYLKRRLLDVTPLIDGIYPSAEFETAFRHAFGPGGKKILLRWS"
     gene            10235..11965
                     /locus_tag="PLM79_12575"
     CDS             10235..11965
                     /locus_tag="PLM79_12575"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOV87193.1"
                     /translation="MKKNLLLLGLAAIAMVVWLPSVLSAQTPPAGPSDILIASGVVTN
                     AQGKPVKEVQVHPLVNGKKLELKEEILTSQDGRYVLRLVLPPGTLPEAKISLEACKPA
                     YANSGEIRLDRVVKEGEDHQGNQVYLANRDVKMARAVTPAFWIASLVLVGVYVLIALE
                     LMHRTLAAFLGASLLLVITYTAGTFNPDYVILTYEDAVRAIDMNVILLLMAMMIIVGV
                     LKRTGVFQWMAYKSFQLARGNVFALSAVLMVVTAVTSAFLDNVTTMLLIIPVTIEIAL
                     TLKIDPVTLLMPEVFASNVGGTATLIGDPPNIMIGSYAKLSFLDFVQNLTFICVICLI
                     FTVVYFLYWYRKEYARAKVGDVQALLKHLREEYRITDKTLLIQGGLILGVTVFLFIIH
                     GALHMEPSIAAMIGAAVLLAVSRTNIVEILEHEIEWPTLIFFMMLFIVVAGAEETGLI
                     QTIADWVNVVSQGSLVVAILMILWVSAVASAIVDNIPFTATMLPISAYLTTTIPGAEG
                     GILWWALALGACLGGNGTMIGASANVVTVGMAERAGHPISFLHYMKVAVIPTIVTIVL
                     ASLWLLFVEI"
     gene            12077..13057
                     /locus_tag="PLM79_12580"
     CDS             12077..13057
                     /locus_tag="PLM79_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOV87194.1"
                     /translation="MPVISRSSFSPPRFLRNAHVQTVVPNMVRAVKGVSYTRVRIATP
                     DDDFLDLDFSPVGSPRTAIVVHGLEGNSRRPYVLGMIRALNARGWNGVGMNLRGCSGE
                     PNRKLRFYHSGDTGDLQTVLDYVWGGNSGGKTALIGFSLGGNIVLKYLGERGSSAANL
                     ICAAAAFSVPCHLSSSSTRMALPSNRFYTARFLRSLRDKITQKMERFPGKIDDEGYEA
                     IRTFHEFDDRYAAPIHGFKDAEDYYARASSKPVLRRIRVPTLLVNALDDPFLAPPCYP
                     YEEARDNPYLFLETPSSGGHVGFWSSGIHGSSWAERRAVEFLEPWGRQTS"
     gene            complement(13062..13586)
                     /gene="tpx"
                     /locus_tag="PLM79_12585"
     CDS             complement(13062..13586)
                     /gene="tpx"
                     /locus_tag="PLM79_12585"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOV87195.1"
                     /translation="MAERFGAVTFQGNPLTLTGNEQKVGNVAPDFEVLDNGLSPVPFS
                     SYRGNVVILTSVPSLDTPVCDLETRRFNQEAGKLGEDVVVLTVSMDLPFAQKRWCGAA
                     GVTQVVTLSDHRDAAFGLAYGLLIKELRLLARAVLVVDRKGIIRHYELVKEVTHEPDY
                     DAALAAAKECASEK"
BASE COUNT         2728 a         4119 c         3993 g         2824 t
ORIGIN      
        1 caggatcccc aggcgttgcc cttccagggg gatgaagtcg tagaagtccc ccccgacctc
       61 gagagccgac cggtagaggg catgaaaccg atatccttcc agctggggaa cccccggggg
      121 caggaagctc cgctgaatgc tcgtggcaaa ggccaggtcg ctctcgatct gctgcttgcg
      181 caacagggcc cggtgtattc gagcgttctc gacggccacg gccgcatggg cgcacaaaag
      241 ggagaggagt tcggcgtctt ccgggagaaa cgcggtcccg tccaccttgt tgatcacctg
      301 ggaaaccccg atcacccggt ccttgatctt cagaggtaca caaaggatgg attgggtccg
      361 ataccccgtc ttccgatcga aatcgggatg gaaccgctcg tcccggtagg cgtccacaat
      421 gatgaggggc tcccccgtct ggaacacgtg accggcgatt ccctcgccct tcttcaaacg
      481 caaaccggtc ttcagcttgt gcgccaccgg cccctgggct acctcgaaga ccaattcctc
      541 ggtcgattcg tccaccagca gaaggctgca ggcgtcagcc cccagaacct cccgggaggc
      601 ggtcatgata tgctccagga cctcctccag ttccagggta gagttgatca gaccggagac
      661 tcgaaagcac tccttcaggt ttctcacgcg cgctcggcat cgcgagagct cggaaagatc
      721 ggaatcttcc aggcggttct tggccgtttc catgaattcg ggcttgatct cctggcggct
      781 gggtacaccc gggacctgag aaaatggcgg cccagggacg agcgccatcg aacccacacc
      841 caggattgaa acggaaatcg ggtcccacga gcagagaagt cagagaacca tttctccggg
      901 ttttcgtcaa tattcttttt gtccgctttc ccttggggtc tatgtccgaa ttgacacatc
      961 ctgtcggctg agataccctt ggcttccgaa cccaaggggg tcccccctct tcacccggga
     1021 ccgttcccgg aggagggagg ctcttttgga cccggcatcc aaacccatca taaacctttc
     1081 acaggagggg cacgatgtta cgatttttgc ttgttttgct cacgggcgtc tttttaattc
     1141 cggtaccctg catgcacgca aacgcggccg atcccccaac ccccaaggtc aaggacctga
     1201 tccttgccac caccacctcc accgtggatt cgggtctttt ggacgtgctg attcccgtct
     1261 tcgagaaaaa gaccggccat cgagtcaaga ccatcgccgt gggaacggga caggccctgg
     1321 ccatggggga aaagggggaa gccgacgttc ttctgaccca cgcccccgaa gccgagaaga
     1381 agctcgtgga ctcgggtacg gtcctgaatt acctgctggt catgcacaac gatttcatcg
     1441 tggtgggtcc tcccgaagac accgccaaga tcaagggcaa atccagcgtg gatgcgttca
     1501 aggccatttc ccaggcccag gccgtcttca tttcccgggg agacgattca gggacccaca
     1561 agagggaact gtccgtctgg aagaaagccg aagtcagcct caccggagcc aagtggtacc
     1621 agcagtccgg gcaggggatg ggggccactt tgctcatggc ttcggaaaag caggggtaca
     1681 ccctgacgga tcgagccacc tacctgagtc agaaaaagaa cctcaaactg gatatcctct
     1741 ccgagggaga caaggccctc ctgaacatct atcacgtgat gcaggtcaac ccggagaaat
     1801 ttcccaaggt gaacggggaa ggagccaagg cgttcgcgga attcatggtg gcccctgaaa
     1861 cccagaaaat gatcggggaa ttcggcaagg acaagttcgg ccagtccctt ttcttccccg
     1921 atgcgggaaa gaccctgtag caacccggcg gaccgatccc cgggaacgag ttcgctccgc
     1981 aaaaccatgc acccaaggtc tttccatccg ggggttgccc ctgcagatcg agggcgaccc
     2041 cccggccgcc gattgtaggc tttccagggg gagggcgggc ggtgcccccc ttcgagcccg
     2101 cctcctcaca tctttccggg agactcccgg cctgcactcc gggcatactc tcgtccccaa
     2161 ccctacaatg gaaagggccc tcccagggcg ccgttgcgcc gacttgggca aaaactccgg
     2221 actgggaaat tgggggcgac tccgctacga tgggccggcg gtcgctcaag gggcttgttt
     2281 tgtctgcggc cctgcgaaac gatggggaca agaaaccact tgggaggcag ttctcccatt
     2341 ccgtcgaaca cccaaacgtt ccggagcggg gttccaccat gaagatgttt gtttggcgtc
     2401 acagcaagaa gctctcgagt tggtccatgt tcgatgagcc tcatatctac cgggacaact
     2461 atctcagggc cgaagtctcc gttctggccg attccctgga ggaagccctg cagtggctcg
     2521 aatcttccgg ggaatggaat atcgaggaac tgagaagaat cgagccgcgg gtcgtcgctg
     2581 cggatcagcc ggggatcgtc tggaaatcca ttgattttgg gtagacgagg cgggtttctc
     2641 aggatcggac ggggtcttcc atggacgtca tgagttcacc cgaagtggtg cgatccatgc
     2701 ccggagggat cccggggtcc gaacttgccg tcattccccg ggcggggcat ggttcgaacc
     2761 tcgagaattt cgacgagttc acccggaaga agacggaatt tccagagaag ctcgaatgaa
     2821 agcacccgtg gaagccctct tttcacgctt tgccggaggg ggaagctctc gattacggat
     2881 aacccggatt ttctgagaca tcctcactct gcctccgagg gaacaaccgc ttccggagcg
     2941 ggctgcgacc gcttctcgga ccaaaaagag ttgctttttg cgattcattc ttcataatga
     3001 gcgagggtct tttcagggcc ttgagctttt tgaccgatgt atccggatga ctaccatccg
     3061 catcccccgg ttttgtttca tgaccgtcac tcttcaaaag gaggtagccg cagtgaaaaa
     3121 gcgacccggt tttcaagact ggtcacgggt tcggagcttc gtgtttttct tatgcctgac
     3181 ggcggccctg ctgccggccc cggcaacccg ggccgccgac gggcaaagac cctcgatggc
     3241 tcccgaaaat ccggctttcc aacagtacat cgaggacgtc gagttcggcc ggttggaacc
     3301 gctcatggtc ggagattttc cctcggggat cgtgcctccg cccgtgaagt ttccccggaa
     3361 ggccatcgag tccgtggagc ccatgagggc cattccgtcc gcttacgacc tgcgcaagaa
     3421 gggccgggtc acttcggtca aggaccaggg aagctgcgga tcctgctggg ccttcgggac
     3481 ctacggttcc ctggaatccc gcctgatgcc cgacaaggcg tggaactttt ccgaggagaa
     3541 cctcatcgaa aaccacggtt ttgacaaggg cccctgcgac ggcggcaatt atgaaatgtc
     3601 cacggcctac cttgcccgtt ggagcggtcc catcaaggaa tccgacgatc cttacaacca
     3661 tgggggagga gtgggcgtgt cggccgcata caagccggtc aagcacgtgg ccgatatcgt
     3721 gttcctgccg gagcgggcga ctcccaagga caacactgcg gtaaagcaga cgatcatgaa
     3781 atacggggcc gtggccggct acatttacta cagcaattcc tactatgact cgaccaacta
     3841 cacctactac tacgggggat cgagcagcgt caaccacggg gtggccattg taggttggga
     3901 cgattcgttt agccgcagca agtttaaagc ctacggcgga aagttgcccg cgggcaacgg
     3961 cgccttcatt gtgaaaaaca gctacgggac gacgtacggg cagaaggggt acttttacgt
     4021 ttcctattac gacaccagca tcgagccgtc catggctttc aacctcgtgg actcccccac
     4081 caagtataat cagatcttcc agtacgaccc cctgggggcc atcaccgggg tcgggtattc
     4141 ctcgactacg gcgtggggtg ccaacatctt caaaagcagc accaccttca cgttgaaagc
     4201 cgtgggcttt cacaccctgg tgcccggcac ttcctacacc gcctacgtgt ataagggggt
     4261 cacggcggga cagccccgga gcgggaccca ggtggccaag gtgagcggca agttccccat
     4321 ggcgggatat cacacggttc ccatcgggtc cgtgggaatc cagtcggggc agaggttctc
     4381 cgtggtggtt tgcttcaaca ctccctccta cacctacccc gttcccgttg aggcacgtat
     4441 agccgggtac agcagcaagg ccacgtccac cacgggacag ggtttcatta gttccgccgg
     4501 aagcacctgg caggatatct acaactcctt tgccaacagc agcgtctgta tcaaggcgat
     4561 gggggtgaaa tagcctggga gaaaacggac gggccgggag accgcgtcta cccggcccag
     4621 cccggaacat gggcccgtgc ggtccgcatg gcgcggtcca ggtcccggta tgcgggttcg
     4681 aaccttgcca ccagattcca gtaccggggg gaatggttcg ggtggaccgt gtggcaaagc
     4741 tcgtggatca aaatatatcg aaccagttcg ggggggagaa agaggagctt gtaattgagg
     4801 ctgatggttc ccctggatga acagctgccc cagcggctct tctggcccct cacttgaagg
     4861 gtccgaaagc ccaggcccgt ttcgcggccg atcctcccga gccagggggc aaggtgggcc
     4921 gtgcccaccc gcttgagcca gggccgcatg accctcccca ggtgacgggc gtctcggacc
     4981 tctcccctca cccgccagtc cactttcccg ttgaggacca gttgaaatcc cggttcgtcg
     5041 tctccggcgg gttccacgac gtagcgctcg tccacggccc ggagccaaat ggaatcgggc
     5101 agggcaaacg gctcttgccc cgagtcgttc cgtcccgaaa accgctggag ggtctcgtgg
     5161 atccagtcct ttttctcgat caggatgccg gggattcttc gtccgtcgaa cccccgggga
     5221 accacgacct cgagtccctt ttcgggggaa atcttcaaaa tcacccttcg cgcccggggg
     5281 tcttcccgaa cggaatagga aatgccgtct ccgagaccct ccttggcggg ccctctcttc
     5341 ttagaatccc aggtcttcat tgatcaggat taccttgatc cgccctttgg gaaacgattt
     5401 ctccacgatg gaccaggcgt tgcaaattcg gtccgggctc ttgcagtctt cgcattcccc
     5461 ggtcttgacg caggggacct tcttgtccag gcgcatggcg ttcacgggag ccgcatacag
     5521 cttgacgcga atcatggcgt cgttgaggtc ggccacgatc ttgttgcgcc ccaccaggat
     5581 gacgacattt cgagggccga acacgatggc ggacacgcga ttcccgatca tgtcgagatt
     5641 gaccagttcg ccgctttcgg tgacggcgtt ggttcccgtg atgaacaggt cggccaggag
     5701 cgcttgtctg cggcgctcga acatggcttc gggggcgatg gacttatcgt acgtgtcgac
     5761 gacctccaac cccggcacgg ccttcatctt ctcgtacagg cccgttccga tgaaggtgag
     5821 ggagcctccc caggagacgc tcttggcacc cgttttgggg acaatctcct cgataaggag
     5881 tctccgagct tcctcgacat tgtcggccac aaaggcttca aagttgtgtt cttcgagtgt
     5941 ctttttcacc tggttcaacc ggatttgcca aaagtgatcg atcggccttt ccatgggacc
     6001 ctccctggtt gatgggcaaa cggtcaaaac ccttcgggcc gaagtccgat cctccggccc
     6061 ccgggtgaat ggggaaaacg gccctgcggg cttgtcagtt ccgcatgcaa tgccgtaata
     6121 tacacgagcg aagccaccat gatgcaaatt gggccggaca tcaagtgtta tcgtccggtc
     6181 aaatcccgaa ccccaggaag aaccgcggac gtctttccgg cccccgaagg ccggaagcgc
     6241 cctttccgtt tccccaatga ctttcccttt gacttcccga ggagtctccc atggcacgat
     6301 tccgccccgt tttcatcgaa ggaagagatc tggacgacac gtggtttcag ctgctggcgc
     6361 aactctacga gcacggaagg cgatacgaga tcaccgccgg gtcctacgag gggtcccagc
     6421 gggttgcata cgactttgtt tccgggttca tccactatcc ccattcgaga cccctggccc
     6481 ctcgggttcc cgaaaacagc cctctgcctc cccccaccac ggacgaggaa atcgacaagt
     6541 acttcgtcaa ctacctgatg tgctcggatg ccgcagtcca tgaagactat cgctacgcca
     6601 cgtgggtcgt ggggggagac tatgagcttc cccggttcca gaaaccggga ctggctcccg
     6661 tggacaagga gacggtccgg gttcccaacc agttggagtg gattgtccgc catttcaggg
     6721 agaaaggaca cggaaacgag cactgctttc ttacggtggg atacccggaa tcgaacttcg
     6781 cttacgatct accctacagc aatcccaacg agcggaaaac cagcccgtgc ctccgagggc
     6841 tggacttccg catcgtagaa ggtcaactgc tcacccatgt catctaccgt tcctgggacc
     6901 tggcctcggg atgggccacc aacatgggag ggttcacgct gctcaacgag tacatggccg
     6961 cggagatcgg ggtcgaaccc ggacccctgt ccttctcgtg caagtccctg cactgctacg
     7021 agcatgcatt cgaatacctg aaactgcgac tgggaaaggc gtgaggtcct tcgtgcccca
     7081 tcatcatcga ggctcagtca atccggggga ccggcggccc ttaccctatt gccgacattc
     7141 ccccgccttc tccttggcct tgcactcgtc gcacaagtcc gagttgggct tggtgctccg
     7201 tgtctcgacg ttgtagctgg tcggatagtt ggaacaattc tcacaccagt gccatttgtt
     7261 gcttttcttg tctttcacat acatgatcat ggccgctctc cttgaggatc cgtgggggca
     7321 acatgcgagg cacaacggaa cccgtcgggg ttccattttc caacattccg agaatacggc
     7381 aaagttctgt tttgtcaaaa cggaaatccg ggggaaagcc cgtgtctttg cccgaggatc
     7441 cgctccgtcc cttcaattcc tgcgagaggt gatcaggatg aacaatcccg ttggtgccga
     7501 ttatgtattg ctggacagcc cggcaattct ctcacgtctg ttctaccccc gagaagactg
     7561 gaggccgggg gactgggagc ccccggggga ggaagtcgcc atcccggtgg aagagggcgt
     7621 cgaggtggga ggaagatttc accttagcga tccccacggc tgcaacctcc tgttttttca
     7681 cggtaacggg gaaattgtgg ccgactatga cgacattgcc cccattttcg gccgcatggg
     7741 aatcaacttc ctccccgtag attaccgcgg atacggacgc tccggcggat ccccttcggc
     7801 atcctccatg atgagagact cccatgagat ctttcgttat gtgcggaaat ggctcgacga
     7861 gaacaagcat tccggtcccc tgatggtcat gggtcggtct ttggggagtg cctcggccct
     7921 tgaactggcc ttccattacc cggaacaact ggatgccgtc attgtggaga gcggttttgc
     7981 atacacgatg gccctgctgc ggtttctggg agtggacgtg gaggggatgg gtttggggga
     8041 ggcggacggg tttggaaata tcgagaagat tcgctccttt ggcaagccga ccctcctcat
     8101 tcacgcggaa ctcgattcca tcatcccgtt ttccgacgcc caggccctct tcgatgcctg
     8161 cggcgcccgg gacaagaccc tggtgaagat cgagggagcc gatcacaaca atcttttcct
     8221 cgtggggctc cccacgtacc tttcttccct caagaccctg gcggacaagg taaaggccgg
     8281 cacctgatcg gcggcggggt caggaccacc gaaggaggat cttttttcct ccggggccaa
     8341 aggcatgccg gaaggctgtt tcaaactcgg cggaagggta gatgccgtca atcaaagggg
     8401 tcacgtccag gaggcgacgc ttgaggtagg agagggccag tcggaggtct ccgcatcggg
     8461 accccgcgat cttgatttcg tccacgacga tccgggccga gttcacggag gatggaaccc
     8521 gggacgtggt ctttagaacc agggtccccc ggggtcgcag gaaggccagg gcttcttgga
     8581 ttcccaggtc gctcccggta gcctccacca ccacgtcgaa ggaacccggc ggggacgggg
     8641 ccgaggggtc gtcttgggga gtagaaacgt tgaagacttc gatcccctgt tttcttgccg
     8701 tctcgagctt ttccgaatgc cttcccagca ggacgagatg aggggtgagg gttcgaaggc
     8761 agagggcaat gaggagcccc agcttgccgt ctcccatcac cagcacccgg tccgagcttc
     8821 ggatatggac ctgctgggaa acgtccagag ccgccgctag gggctccgcg aataccgcct
     8881 cctcgtcccg gatgccggct tccacgagat gaacgttggc ttcgggaacc ttgagatact
     8941 gggcgaatgc gccgtcccat ccctgaatgc ccacggccct tcgggacagg cagtgcttgg
     9001 gaacatcggc ccggcaccag gaacactcac cgcacccgat attgatgtcc gccaccactc
     9061 tgctgccctc gatttctttt cgcccgggag ccctttccac ccggcccaca aactcatggc
     9121 ccggcactcc ccggaagccg tagtagccct cgaacagttc gcgatccgtg cggcagatcc
     9181 ccgtcaggag ggtccgtatg aggacctccc cgggcagagg ttccggtatg ggcacttcca
     9241 ccagccgaat ggaaccttca tcaaaaacca cggcattcat gcacgaagaa acagacattt
     9301 gtccttcagt cgttcggagt tctagggagg aggatacgct gcaagtgaat cttacgattg
     9361 gttttctgtc aacacaaatt tccggcatga ctcccgaccc cggccatggg cgtccgctgc
     9421 cctatggaac aggggcttcc tggtaagcgg gagaggggac gaggacccgg aggccttgaa
     9481 ccctttttct ttgacatgtg gattggagac cggttatatg acccgcaaga caaaatgaat
     9541 aagggtcgtg ggtccaagag atagtggtgc tcttggaggt taaggttcag aggatagtcg
     9601 ggccgctatt cacccccggg gtatgggtgg agggcggctt tttcgtcgtg ttcgggttgc
     9661 ggccgtcaag gaaagctcag gttgatcagg ccggttcccg agtgcggtcg atttttcgca
     9721 agagcaggta aatccgggac cgccgtccaa ttcccgtcac gggagcggat ggcggttttt
     9781 ttgtgctcga ttcgaaagga caaaccgccg gaacaaggga acagtaccgg gggtccggaa
     9841 gtgaaggcca ctttcggagg atagacgcag gggatagtcg ggccgctatc cgtctcaccg
     9901 aacacgggtg gggaggagag cgatcctttc ccggggctga taccccgttg ccgcctcccg
     9961 cccaccgaaa cacgggaggt gaacccatgg atgccggcaa tactccgtcc gctgttcgca
    10021 ggacaatccc cattccagaa ccgcgtttct ccaactccca aggagaagac tatgtcccgg
    10081 cccgaatgga cacagtgcct gaatgcggat tcggtgtgtc tgccgccgtc tccgggtcca
    10141 actccgcaac aacggacccc atcggctgca tcctggtccc ggatcgcggc cgggaaggca
    10201 ttcaccccat ccatccacag aaggaatcag tcgcgtgaaa aagaatctcc tgttgttagg
    10261 cctggcggca atcgccatgg ttgtatggct tccctcggtt ctctcggccc agaccccgcc
    10321 cgccgggcct tcggacatcc tgatcgcatc gggagtcgtc accaacgccc agggcaagcc
    10381 ggtcaaggaa gtgcaagtac atccgctggt aaacggcaag aagctggagc tcaaggaaga
    10441 aatccttacc tcccaggacg gaagatacgt gttgcgactg gttctccctc ccgggaccct
    10501 gcctgaagcg aaaatctccc tggaggcatg caagccggcc tacgccaatt ccggggaaat
    10561 ccggctggat cgcgtggtga aggagggaga ggaccaccag ggaaaccagg tgtacctggc
    10621 gaaccgggac gtgaagatgg cccgtgccgt caccccggcc ttctggatcg cgagcctggt
    10681 ccttgtaggg gtgtatgttc tcattgccct cgaactcatg caccggacct tggcggcctt
    10741 tctcggggcc tcccttttgc tggtcattac ctacaccgcc gggactttca acccggacta
    10801 cgtgatcctc acctacgagg acgccgtccg ggccatagat atgaacgtca ttttgctcct
    10861 catggccatg atgatcatcg tcggggtcct caagaggacg ggggtgttcc aatggatggc
    10921 ctacaaatcc ttccagttgg cccgggggaa cgtcttcgcc ctttccgccg ttctcatggt
    10981 ggtcacggcg gtgacttccg ccttcctgga caacgtaacc accatgctcc tcatcattcc
    11041 cgtgaccatc gagatcgccc tcaccctcaa gatcgatccc gtcacccttc tcatgcccga
    11101 ggttttcgcc tccaacgtcg ggggcacggc caccctcatc ggggaccctc ccaacatcat
    11161 gatcggctca tacgccaagc tttctttcct ggatttcgtc cagaacctca cgttcatttg
    11221 cgtcatctgt ctcatcttta ccgtcgttta ttttctctac tggtaccgga aggaatacgc
    11281 ccgggcaaag gtgggggacg tgcaggcact tctcaagcac ctgcgcgagg aatatcggat
    11341 taccgataag acgctcctca tccagggagg gctcatcctg ggggtcacgg tcttcctgtt
    11401 catcattcac ggagcgctcc acatggagcc cagcattgcc gccatgatcg gggccgccgt
    11461 gctgctggcg gtgagccgca ccaacatcgt ggaaatcctc gaacacgaaa tcgaatggcc
    11521 caccctgatc tttttcatga tgctcttcat cgtggtggcg ggagccgaag aaacggggct
    11581 gatccaaacc atcgccgact gggtgaacgt ggtgtcccag gggtctctgg tggtggcaat
    11641 cctgatgatt ctgtgggttt cggccgtcgc ctcggccatt gtcgacaaca tccccttcac
    11701 ggcgaccatg cttcccattt cggcctatct caccaccacc atcccggggg ccgaaggggg
    11761 catcctgtgg tgggccctgg ctttgggtgc ctgcctgggc ggcaacggaa ccatgatcgg
    11821 ggccagtgcc aacgtcgtca cggtggggat ggcggaacgg gcggggcatc ccatctcgtt
    11881 cctgcactac atgaaggtgg cggtcatccc gaccatcgtc accattgtgc tggcctctct
    11941 gtggctgctt ttcgtggaga tttagaaaag ggaagaggag acctggaatc cgggacgatc
    12001 ttgtggtagg aacggggagg tcgcgactca ttgccgccga gaatttccat gattcccaag
    12061 ggtcgaggta tctcgcatgc cggtgatttc caggtcgtcc ttttctcctc cccggtttct
    12121 gagaaacgcc catgtgcaga ccgtcgttcc caatatggtt cgagcggtga aaggcgtctc
    12181 ctacacccgg gtgcggattg ccactcccga cgacgacttc ttggacctgg atttttcccc
    12241 ggtggggtcc ccccggaccg ccatcgtggt ccacggactc gagggaaact cccgcaggcc
    12301 ctatgtgctg gggatgatcc gggcgttgaa cgcccgtggg tggaacgggg tcgggatgaa
    12361 ccttcggggc tgcagcgggg aacccaaccg aaagctgaga ttttaccaca gcggggatac
    12421 cggggacctg cagacggtgc tggattatgt ctggggcggg aattcgggcg ggaaaacggc
    12481 cctaatcggt ttcagcctgg ggggaaatat cgttttgaag tacctggggg aacggggaag
    12541 cagcgcggcg aatctcatct gcgccgccgc cgctttctcc gtcccgtgtc atctttcctc
    12601 gagctccacc cggatggccc ttccttccaa ccgtttttac acggcgcgtt ttcttcgctc
    12661 cctccgagac aagattacgc agaagatgga aaggtttccc ggaaagatcg atgatgaagg
    12721 ctacgaggcc attcgaacct ttcacgagtt cgacgaccgt tacgccgctc ccatccacgg
    12781 attcaaggat gccgaagatt actacgcccg ggcaagcagc aaacccgtcc ttcgccgcat
    12841 ccgcgtgccg accctcctgg tgaacgccct ggacgacccc tttcttgctc ccccgtgcta
    12901 tccttacgag gaagctcgag acaatcccta tctgtttctg gagaccccgt cctcgggcgg
    12961 tcacgtgggg ttttggtcct cgggaatcca cggctcgagc tgggcggaac gtcgggcagt
    13021 ggaattcctc gaaccttggg ggagacaaac ctcctgaaag gctatttttc ggacgcgcat
    13081 tcttttgcag cagccaacgc cgcgtcgtaa tccggctcgt gggtgacttc cttcaccagt
    13141 tcataatggc gtatgattcc cttgcgatcc accaccagga ccgcccgggc cagaaggcgg
    13201 agttccttga tcagcagccc gtaggccaat ccgaaagccg cgtccctgtg gtcggacagg
    13261 gtgacgacct gggtcactcc cgccgcaccg caccaccgtt tctgggcgaa gggaagatcc
    13321 atgctgacgg tcaatacgac cacgtcttcc cccagctttc ccgcttcctg gttgaaacgt
    13381 cgggtctcga gatcgcacac gggggtgtcc agggacggga ccgaggtgag aatgacgacg
    13441 tttccccggt acgatgagaa cgggacgggc gataagccgt tgtccagcac ttcaaagtcc
    13501 ggggcgacat tgccgacttt ttgctcattt ccggtgaggg ttaggggatt tccctgaaag
    13561 gtgaccgcgc caaatcgttc cgccatagga cctcctcgcg agttttgggt gattgtggaa
    13621 ctttcgttga aggagccggg agtttcttat tcgcaaccgg acag
//
LOCUS       DAOOVC010000104        13281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3102_length_13281_cov_6.444579, whole genome shotgun sequence.
ACCESSION   DAOOVC010000104 DAOOVC010000000
VERSION     DAOOVC010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13281
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3102_length_13281_cov_6.444579"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            464..1849
                     /locus_tag="PLM79_12590"
     CDS             464..1849
                     /locus_tag="PLM79_12590"
                     /inference="COORDINATES: protein motif:HMM:NF015905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HOV87196.1"
                     /translation="MALVTEIAEFVSRRSYEDLSENARQQLKVRVLDSLACAVGALDG
                     EPVKRVREQMEDFGGNPRCTLIGGQRTSPDRAAFYNGALVRYLDFNDSYLARHETCHP
                     SDNLGAVLAASEYAGKTGKDFLAALAAAYQVQCRLSDAAPVRSKGFDHTTQGSYAVAA
                     GVSKALGLDVEKTAHAMAISGTAFNALRVTRTGALSHWKGLAYPNTAFGATHAAFLAM
                     RGITGPAEVFEGNKGFMETISGPFEIDWSREDLERVTTTIVKKYNAEIHSQSAIEGIL
                     EMKERFGFRGEDILGIEVNTFDVAFHIIGGGEEGDKTAVATKEEADHSLPYILAAAVL
                     DGSVMPEQYRLERIGRRDVQDLLRRVVVRPRTRLSNRFPLEMPCRLTVRLKNGETLEH
                     EKADYEGFHTRPLPWEGVVRKFDLLAGPHVSGDLGTSIRQAVGELESIRVEELTRLLA
                     RVGSAGEGRNP"
     gene            complement(2706..3332)
                     /locus_tag="PLM79_12595"
     CDS             complement(2706..3332)
                     /locus_tag="PLM79_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOV87197.1"
                     /translation="MDLIASATGADALDRSFKATCCGAGIGIPKKEIGLALMDRLLTG
                     ATACGAHAVVVCCPLCRTNLDLHQPKVLRKKGMNFPRPLPILYYTELLGIAFGLDSVR
                     SGFKCHFVNPTPLLKGRCRACEKTCPVQAVCLESQAREWGLEAGAVVLSGGFEPALVP
                     QTGEYGHGRYAKVVTALEYERMLSATGPFGGHLRGPSDRQKPRRTAWI"
     gene            complement(3221..3787)
                     /locus_tag="PLM79_12600"
     CDS             complement(3221..3787)
                     /locus_tag="PLM79_12600"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOV87198.1"
                     /translation="MQSMSWDYRGSIRHVMEALDIRLTEIPDWTCCGATAARSLDEHM
                     AVILPARNLARAANFGLDPAVACPMYFRRLSRTREVLLHERADIAWSIPKAPVVLDLA
                     RTLAEPPFLDRIRRIARGALEGLRVVCYYGCQVVRPPGSRDMQTTKTPGTWTSLRPPR
                     EPTLSTDPSRPPAAERGSESPRRKSASP"
     gene            complement(3842..4420)
                     /locus_tag="PLM79_12605"
     CDS             complement(3842..4420)
                     /locus_tag="PLM79_12605"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV87199.1"
                     /translation="MNPSGSTLQVGGRERLWSQALAADSGIQVGSCCQCLRCTNSCPV
                     GPFMDLKPHQVVRLVQLGEKEQVLQSSAIWICLSFEMCSTYCPNEIDVAALMGRLKIS
                     VVSSCKKPAERDIALFHRVFLEVLHAHGRMNDLQLLRRFKLESLLQGRLPGQEDLAED
                     LSLAWEPWKRRRLRVLPERSRGAVEIRKVLLQ"
     gene            complement(5092..5559)
                     /locus_tag="PLM79_12610"
     CDS             complement(5092..5559)
                     /locus_tag="PLM79_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HOV87200.1"
                     /translation="MKMWVDADACPGPIKDLILRAARRLQVPAVFVANKHIGLPDSPY
                     LSAVRIGADPEAVDQYLVRQAREGDLVVTQDIPLASALVRKGVVVITPRGELHTGENI
                     GERLAMRNFMQEIREAGVITPPHKPFSSRDKQRFSDTLDRELTRLFNAKSPRG"
     gene            complement(5659..6837)
                     /locus_tag="PLM79_12615"
     CDS             complement(5659..6837)
                     /locus_tag="PLM79_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87201.1"
                     /translation="MKALLSFLMLILLFPPQPLSAGDVESFNSRLFDLQYFTRPFERA
                     AVAGGAWEGAEGSAASILQNPAALALVHKPELLTYWTFNHLNGQTYALKRVIPPGPGH
                     PKVERICERPTARLSDMGVYQAFRLESVPGTFGIGADALWNDLGDNDLSRIEQSGFRV
                     GLSWGVPLGDDLSVGYGLTYLDDTWHWDVTWAKFSPGIPVPQNVDYLLKSSASSWRHR
                     FGLRGNVGSSFRYGLEADLGHGSTDNEWNGRDTGGDNAMHHYGIRIGLEYSLSSKAVL
                     AVEGEWHETSIEFGRHSPDIGRDDAVAKWDTSVIRPMLGFKYSVTDAWQLLAGYRYSR
                     FNTVDLCTHGADTDYGTFSLGTAFSLFHKRLSIRWNLEYSMVDPDGEIMNLLSLNAVF
                     "
     gene            complement(6903..8321)
                     /locus_tag="PLM79_12620"
     CDS             complement(6903..8321)
                     /locus_tag="PLM79_12620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOV87202.1"
                     /translation="MTEKHPPQPIEPPEKPGLAQWLKRRILGKEQDALVPPAAVEDAE
                     LPESVGRYVIRGILGQGSMGVVYLATDPYIQRTVAIKVSRSTIGATGDKANSFRQRFF
                     LEAQSAGRLSHPNIVAIYDAGIYRNSYYITMEYIDGHTLERFCTQGALLPVRKVLEIL
                     YVACRALDYAHRKGIVHRDIKPSNIMLTDTGTVKITDFGIAHIKSEGTMTMGIVGSPS
                     YMSPEQVKEENLDQRSDIFSTGCVLYELLTGEKAFPGDQYYSILYRIAQEDPKSILEI
                     RGDLPELLWDICRRALAKAPADRYQSCLDFALELRAAIRSIREARSRDTPDTTAELLC
                     GVSFFEDFTPEQVRQILDASSLIKVFSGRVIVTEGQIDDCFYVLLSGGVTVQKGGKDL
                     AVLRRGDCFGEMAYLSSRAREATVVAQTDCILLKISATLLDASPESLQILFLRKFALT
                     LVQRLSRAQKASTGAGAPKTTE"
     gene            8735..9271
                     /locus_tag="PLM79_12625"
     CDS             8735..9271
                     /locus_tag="PLM79_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87203.1"
                     /translation="MWKHILIAGVVLVVGLMAVQSPKTEVRIEGRNISIDSGSHHLEG
                     TLDPEATYLIFIKDEGSTVNTFSGDAFVTVLPLKTAEQLRAQFGDFFHCNSPGAGQAM
                     RSMLSVILVSDLEAVKEKVSRAMSLVRKSQIPVVRFRGFRVHVTRQTYAKMEVVDHTG
                     TTLFYLNDFEILKSNYLQ"
     gene            complement(9287..10834)
                     /locus_tag="PLM79_12630"
     CDS             complement(9287..10834)
                     /locus_tag="PLM79_12630"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HOV87204.1"
                     /translation="MKTPKVRKPFVVCTAAFFAFLLGKLLLPSPAAAQPDVDSELRAA
                     PLNAAFVDYVRKSASGGLESRTFAGRFLGYVPPPVDLSSVNRGPLSPHMGSAALFPPK
                     FDLRAVKRVTPVRDQGRCGSCWAFGTMASLESCILKDGEKWDFSEADLNQNHGFDDRI
                     CEGGTFFMSSAYLARWSGPFAEATYPYPFAGTQSSQHASDPSVKKHLQEVLFLPNREG
                     PTDNGAIKWAVFNHGAVAMSFYVKGAYYNSEKSSYYCPDIEENNHGVAIVGWDDHYDR
                     SNFKTMPPGNGAFIVKNSWGEGWGDRGYFYLSYYDINVRDLTLFGGTESAANYKMAYQ
                     YDPQGWVVSIGFNSHTAWMANIFQGSPEGSRISAVGFYTPEKDCEYDIYVYDRVAVDY
                     DRIKKNSPTDGKRVFALTRQTMPLAGYHTVKLPKRVPITPFKKFSVVVRLTTSNSTEP
                     IAVECPIRVGASPNSFAAGANAMAGQSFVSADPKKKGWSDLTTRTDSSDQIDFLKANV
                     CLKAFAK"
     gene            11518..12807
                     /locus_tag="PLM79_12635"
     CDS             11518..12807
                     /locus_tag="PLM79_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87205.1"
                     /translation="MLSLDMKTIVFSYLANSTVCLLVLFLLWRQSRKRFDGIGFWVLD
                     YLFQTAGLALIVLRGSIHEWLSVVVANTLIVGGALLGYIGLERFVGKRSSQVRNILVL
                     GAFVTIHAYFAFVQPNLAARNLNLSLGLLILCGQCAWLLLYGVEPGMRSLTRLVGVVF
                     GLYSLLSVVRIVESLVGPNPGTDFFRSGAFDRSVLLSYQVLFLLLTYSLVLMVNKRLL
                     GDIKIQEEKFAKAFRHSPNAITLTRLFDGTIMEVNEGFSKITGYEYGEAIGKTTVDLH
                     LWNSERDRLSAVEELAKTGRIQDRELQLRKKSGEIVTGLLSAEVFRMNHREFILSSLT
                     DISDRKRAEAEREEHIRELRQAMARVKQLSGLLPICASCKKIRDDQGYWTQIEAYIRD
                     RSEAEFSHGICPECAKKLYPEISGRVLSKGTTDDRGQ"
     gene            13081..>13281
                     /locus_tag="PLM79_12640"
     CDS             13081..>13281
                     /locus_tag="PLM79_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87206.1"
                     /translation="MTPEGLPGINQGLMVAKPQVGKRRPAENQRLIRQMSTPGLKKGN
                     GNGKAPTCLTPIEQEGRTSRVHV"
BASE COUNT         2662 a         3882 c         4181 g         2556 t
ORIGIN      
        1 cttcccccag cccgccgcca caagcgattg atttctttaa ttctccgtcc gggtcgcgcg
       61 gttccgtcca cgagacgggc gggaccaccg cccgagaggg gagtccgccc cttcaagcgc
      121 cgccgtggga ggcggtgctg ccgtttgttt ccatgttcct ccgcagcccg atgtctaccc
      181 tcctttgaaa acgcccgaaa tcggccttct acccaggtga tttcccgtcc cgcggcttcc
      241 cgcttccttg cttgggagag gaggggacgt tcggtccgcg gcatcccagg gggcggcggt
      301 agggcggtcg tggatgatta tcatacactc aacccgcaga ttcacccgtc gcgggcaggg
      361 cggaggccgg cctcgaaggg aagggctcct ttgcccgagg atgcgcaccg tgcggcgtgt
      421 cgtgcggcat gggaagaagc tcgatacaat gggaggaaaa accatggcct tggtgacgga
      481 gatcgcagag tttgtctccc ggaggtccta cgaggacctt tccgagaatg cccggcagca
      541 actcaaggtg agagtcctgg acagcctggc ttgtgccgtc ggagccctcg atggagagcc
      601 cgtgaagcgg gtgagggaac agatggagga cttcggagga aacccccggt gcaccttgat
      661 cggcggccag cgcacgtctc cggatcgtgc cgccttttac aacggtgccc tggttcggta
      721 tctcgatttt aacgacagct atcttgcccg gcacgagacc tgccatccca gcgacaacct
      781 gggagcggtc ctggccgcat cggaatatgc gggaaaaacg gggaaagatt tcctggcggc
      841 ccttgccgcg gcctaccagg tccaatgccg cctcagtgac gcggcccccg taagaagcaa
      901 gggcttcgac cacaccaccc agggatccta tgcggtggcc gcgggggttt ccaaggcgtt
      961 ggggctggac gtggaaaaga ccgcccacgc catggccata agcggtacgg ccttcaacgc
     1021 cctcagggtt acccggacgg gagccctctc ccactggaaa ggcctggcct atcccaacac
     1081 cgcctttggg gcaacccacg ccgcctttct tgccatgcgc gggattaccg ggcccgcgga
     1141 agtcttcgaa ggcaacaagg gtttcatgga aaccatttcg ggtcccttcg aaatcgactg
     1201 gtcccgggag gacctcgaac gggtcaccac caccatcgtc aagaaataca acgccgaaat
     1261 ccattcccaa tccgccatcg aggggatcct ggaaatgaag gagcgattcg gcttcagggg
     1321 ggaagacatc ctgggcatcg aggtgaatac cttcgacgtg gcgttccaca tcatcggggg
     1381 cggagaggaa ggcgacaaga ccgcggtggc caccaaggaa gaggcggatc acagtctgcc
     1441 ctatatcctg gcggccgccg tcctcgacgg aagcgtgatg ccggagcagt accgcctcga
     1501 gcgcatcgga agacgggacg tccaggacct gctgaggcgg gtggtcgtcc gtcctcgaac
     1561 ccggctcagc aaccgtttcc ccctcgagat gccctgccgc ctgacggtcc gcctcaagaa
     1621 cggggaaacc ctggagcacg agaaagcgga ttacgaaggg ttccacaccc ggccgctccc
     1681 gtgggaaggc gtggtccgaa agttcgacct cctggccggc cctcatgtga gcggggacct
     1741 gggcacgagc atccggcagg ccgtcgggga gctcgagagc attcgggtgg aagaattgac
     1801 ccggctcctg gcacgggtcg gttcggccgg cgaaggacgg aacccttgag cctggaggga
     1861 gagaatcatg aatgatacca ggttgcgccc aaggtcgccc tgacatgctg agtaaagatt
     1921 tcgagcaaag ggctgagtcc tgcggacggg cagtttcggt ttgaatgcac cttcgtatga
     1981 caatgggcag gttgatgaga aatccaaaaa tcccggctcc cacaaaattc cggaatccca
     2041 cggccatcag gaaatagaag gtccattggt gagggatggc aatggatttt tggcagacct
     2101 cgcacttggc ccgggtcagt ttcatgaaat cccgggcatc cagggttggc agggtcaggg
     2161 gaaccacttc catggcggaa aagacggcgg cgcatgcggc ggcggcactg gcaacggatt
     2221 cggggatgtc ccgggggccg tccagggtcc cgcatacgta gatgcccacc cgggaggtat
     2281 gacagggccg ttcgaggtcg gggggccacg aacccatagg agttcaattc cagccccagg
     2341 cttttgagga gaggcgcgag gctcgaggaa ggtttcatcc ccgccgaaag caccacctgg
     2401 tcgaattcct cccgcctgtg ctcgtagccg gtacggtcgt aatattcaat gaacaggttg
     2461 ttgttgacgg ggttccagat cacccgggag ggcatgcttc ccacgaagcg cacgccgtat
     2521 gggtccttgg ccctgaggac gtaccggtca aagtcctttc cctgaccccg aatgtccagg
     2581 aagaagatga ccgcttcgct ttccgggtcg tggtcccggg tgaggacggc ctgcttgacg
     2641 gcggccatgc agcagacgga agaatagaag tttcggcctc gagcgctgtc cctcgaggca
     2701 acacactata tccaggccgt tctgcgaggc ttttggcggt ccgaaggccc gcggaggtga
     2761 ccgccgaagg gaccggtggc cgaaagcatg cgctcatatt cgagggcggt gaccaccttc
     2821 gcataccgtc catggccgta ttcccccgtc tggggcacca gggcgggctc gaagcccccc
     2881 gaaagaacca cggcccccgc ttcgaggccc cattcccggg cctgactctc caggcaaacg
     2941 gcctgtacgg gacaggtctt ctcgcaggcc cggcatcgtc ccttcagcag aggagtggga
     3001 ttcacaaagt ggcacttgaa tccggaccga accgaatcga gcccgaaggc gatccccagg
     3061 agttccgtgt agtaaagaat gggcaaaggc cgggggaaat tcatgccctt tttgcggagg
     3121 accttcggct gatgaaggtc caaattcgtc cggcacaggg gacagcagac cacgaccgcg
     3181 tgggccccgc aggcggtcgc cccggtcagg agcctgtcca tcagggcgag gccgatttcc
     3241 ttcttgggga ttccgatccc cgctccgcag caggtggcct tgaaggatcg gtcgagagcg
     3301 tcggctcccg tggcggacgc aatgaggtcc aggtgccggg ggttttcgta gtctgcatat
     3361 cccgtgagcc gggggggcga accacctggc aaccgtaata acaaaccacc cgcagaccct
     3421 cgagggctcc ccgggcgatc cgtcggatgc gatccaggaa aggcggctcg gctaaggttc
     3481 gcgccaggtc cagcaccacc ggtgctttgg ggatggacca ggcaatatcg gcccgctcgt
     3541 ggagaagcac ttccctggtc cggctcagac gccggaaata catgggacag gcaacggccg
     3601 ggtcaagccc gaaatttgcc gcccgggcca gattcctcgc aggcagaatg acggccatgt
     3661 gctcgtcgag gctgcgggcg gcggtcgccc cgcagcaggt ccagtcgggg atttccgtca
     3721 accggatgtc cagggcctcc atcacgtgcc ggatggatcc ccggtaatcc cagctcatgg
     3781 attgcaaggc acagccgggg gtaataggcc atcgttttta tgaaggacct cccttccgac
     3841 cttactggag gaggaccttg cgaatctcga cggctcctcg gctccgttcg ggaagaactc
     3901 tgaggcgacg gcgcttccac ggttcccagg ccagggaaag gtcttcggcc aggtcttcct
     3961 ggccgggcag gcgcccttgc agcaagctct cgagcttgaa acgccggagc agttggagat
     4021 cattcatgcg gccgtgggca tgaagaacct ccaggaagac ccggtgaaac agggcgatgt
     4081 cgcgttcggc gggtttcttg caactggaga ccaccgagat cttgagacgg cccatgagtg
     4141 ccgccacgtc gatttcgttg gggcagtacg tcgagcacat ctcaaacgag aggcaaatcc
     4201 agatggcgga ggattgaagg acctgctcct tctcacccag ttggaccagg cggaccacct
     4261 ggtggggctt caggtccatg aaagggccga cgggacagct gttggtgcag cgcaggcatt
     4321 ggcagcagga ccccacctga attccgctgt cggcggcgag ggcctgagac cacagccgtt
     4381 cgcgaccgcc tacctgcaat gtcgatccgg acggattcat gttcatgctc cctggcagga
     4441 atggggtttc agaggagccg gggcggtcgg tcgggcagga acgccgcatc cgaagggttc
     4501 tcgaacctcg aggggttcgg ttcggcaacc acgggaacgg actgggaaaa gcgaaaatgg
     4561 acaacggaaa cggcgaaaag tttctcctct tcttcgttac ggccaaacgg gcccttcacc
     4621 cgaaagccac gccccgggca agtgtgcttc caggagaaga actctctcaa aattcccctc
     4681 cgggatgtct tttacgcccg gtgggcgggg gcatgaggct tacatgagcc ctcctcccgg
     4741 ggggaaaaaa gcaaaaccga cccttccgat agccattcat ttcccttggg taaccccccg
     4801 gctttgccga gggactccca gagtttgaca gttccgggag tccgttttgc cagtaggcgc
     4861 tgtagcacca accaagcctg aacacagtat tccggtcacg ctgttacggc tgccttgaat
     4921 catgatgatg aaatgaagca cagctgacct ggtgagcgcg tagcagcacg agtttacaca
     4981 gtgcttcaag ccgctttgag cggtccacgg ttttacaagc ctccggcttt gccggaggta
     5041 cctgactcta ccgaaggaaa cagccaaagg aaacaaaggg gggaagggtc gctatccccg
     5101 gggagatttt gcattgaaga gccgggtgag ttcgcggtcg agggtgtccg agaaccgctg
     5161 tttgtctcgt gaggaaaacg gcttgtgagg gggagtgatc acccccgcct cccggatttc
     5221 ctgcatgaaa ttgcgcattg caagccgttc cccgatattc tccccggtgt ggagctcccc
     5281 tcgaggcgtg atgaccacca ctcctttccg caccagggcc gaggccaggg gaatgtcctg
     5341 ggtgacgacg aggtcccctt ctcgagcctg ccggaccagg tattggtcca cggcctcggg
     5401 atctgcgccg atccggaccg cggacaggta aggggagtcg ggaaggccga tgtgtttgtt
     5461 ggccacaaag actgcgggga cttggagtct tcgggccgct cgaaggatca ggtccttgat
     5521 tggacccggg catgcgtctg catccaccca catcttcatt ggcgggcttt caagggttcg
     5581 ggagtcgtct tccggggccc gcgccgggcc ccggaccgga ggggcgtttc ccattggacc
     5641 cgcccccggg cacatccttc aaaacactgc gttcaaactc agcaaattca tgatctcgcc
     5701 gtcggggtcc accatggagt attcgaggtt ccagcgaatg ctgaggcgct tatggaacag
     5761 ggagaaggcg gtcccgaggg aaaaggtgcc gtagtcggtg tccgcgccgt gggtgcacag
     5821 gtccacggtg ttgaacctgc tgtagcggta cccggccagg agctgccagg catccgtgac
     5881 cgagtacttg aaacccagca tgggacgaat cacggaagta tcccacttgg ccaccgcgtc
     5941 gtcacgaccg atgtcgggag aatgccgccc aaactcgata ctcgtttcat gccattcccc
     6001 ttcgacggcc aggacggctt tcgagctcag gctgtactcc aagccgattc gtatgccgta
     6061 atggtgcatg gcgttgtcgc ccccggtatc ccttccgttc cattcgttgt cggtggaccc
     6121 gtgtcccagg tccgcttcca gcccgtagcg aaagctcgat cccacattgc ccctgagccc
     6181 gaaccggtgc cgccaactgc tcgcgctgct tttcaacagg taatccacgt tttggggaac
     6241 ggggatgccg ggtgaaaact tcgcccacgt gacgtcccag tgccatgtgt cgtccaggta
     6301 ggtaagcccg tagccgacgg acaggtcgtc tcccaggggg accccccagg ataggcctac
     6361 tcggaagccg gattgctcga ttcgggacag gtcgttatcg cccagatcgt tccacagggc
     6421 gtccgccccg atcccgaagg tcccggggac ggattccagc cggaaggcct ggtagacgcc
     6481 catgtccgac aatcgagccg tgggtctctc gcaaatccgt tccaccttgg gatgacccgg
     6541 tcccggcggt atgacccttt tgagcgcgta ggtctgaccg ttgagatgat tgaaggtcca
     6601 gtaggtgagc aattcaggct tatggaccag ggccagggct gcggggttct gaaggatcga
     6661 ggcggcggag ccttcagccc cctcccacgc cccgcccgct acggcagcgc gttcgaaggg
     6721 ccgggtgaag tactgcaggt cgaagagccg ggagttgaag gattccacgt ccccggcgct
     6781 caggggttgg ggcgggaaga ggaggatcaa catcaggaag gacaagagag ctttcatggg
     6841 aacctccgac ggcggccgag aaggaacggt cggctaagag cagtcagcga tgagcgggac
     6901 ggtcattcgg tggtcttggg tgcgcctgca cccgtcgatg ccttctgagc cctcgagaga
     6961 cgctggacca gggtgagggc aaattttctg agaaacaaga tctgcaaact ctcgggggat
     7021 gcgtccagca gagttgcgct gatcttgagc aggatgcagt ccgtctgtgc gacgacggtt
     7081 gcttcccggg ctcggctgct gaggtaggcc atctccccga agcagtctcc tcttcgcaaa
     7141 accgcgaggt ccttgccgcc cttttgcacc gtcacacccc cgctcaagag gacgtagaag
     7201 caatcgtcaa tctggccctc ggtcacgatc acccgccccg aaaagacctt gatgaggctg
     7261 ctggcatcca ggatctggcg tacctgttcg ggcgtgaagt cttcaaaaaa agacaccccg
     7321 caaagcagtt cggcggtggt gtccggagtg tctctggacc gggcttccct gatgcttcgt
     7381 atggctgctc tgagctcgag ggcgaaatcc aggcaggatt gatagcggtc ggccggggcc
     7441 ttagccagag ccctcctgca aatatcccac agaagttcgg gcagatcgcc gcgaatctcg
     7501 aggatgctct tggggtcttc ctgggcaatc cggtacagga tggaatagta ttgatctccg
     7561 ggaaaggcct tctccccggt caggagctca tacaaaacgc aacccgtgga gaagatgtcg
     7621 ctccgctggt ccaggttctc ttccttgacc tgctcgggag acatgtagct gggacttccg
     7681 acgatgccca tggtcatcgt cccttcggac ttgatgtgag cgatgccgaa atccgtgatt
     7741 ttcacggtcc cggtgtcggt gagcatgatg ttggacggct tgatgtccct gtgcacaatc
     7801 cctttccggt gcgcataatc aagggcacgg caagccacgt agaggatttc cagtaccttg
     7861 cgcacgggca gaagagcccc ctgggtgcaa aaccgctcga gggtgtgtcc gtcgatgtat
     7921 tccatggtga tgtagtagga atttcggtaa atcccggcgt cgtagatggc gacgatgttc
     7981 gggtgtgaca gcctcccggc ggattgggct tcgagaaaga acctctggcg gaagctgttg
     8041 gccttgtccc ccgtcgcgcc tatggtggag cgggagacct tgatggccac ggtccgttga
     8101 atgtaggggt ccgttgccag gtagaccacg cccatgcttc cctggcccaa aattcctcga
     8161 attacgtagc gaccgacgga ctcgggcagc tcggcatcct cgacggcggc cgggggaacg
     8221 agggcgtcct gctccttgcc caaaatgcgc ctcttcagcc actgggccag gccgggtttt
     8281 tccggaggtt caatgggctg tgggggatgc ttctcggtca tgggcttgtt cctctccaaa
     8341 gccgcggagg aaggccgttg attggaaagt tgaccgcgaa cctgcagggc attgggaccg
     8401 aatcgggacc cgtcaagtca agaaaagtaa tgccacaact gatgggaatg aaccatgata
     8461 aaacgcatcg tccccgggtt ttgggtgcat tgtgtgcttc ggactcagat gaacctcatg
     8521 ggaaagggac ccggtgccga tcattgaata ggtcccatga tgcgcgactc cccggggacg
     8581 cgatccccat gggacccgcc cccggatgct cgatacaggt ttccgccaag ttcagggcga
     8641 tcctgccgtg ggggctcggg ggccggaccc ccgcaaccgc ggtggggagg gggcacaccc
     8701 agattgcccg tcgagacgtc gaaaggattc atccatgtgg aagcatatcc tcatcgcagg
     8761 ggttgtgttg gtggttggcc tgatggccgt tcagagtccc aagaccgagg tccgcatcga
     8821 ggggcggaac atcagcatcg attccgggag ccaccatctc gagggaactc ttgatcccga
     8881 agccacgtat ctcatcttca tcaaggacga gggatccacc gtgaacacct tttccggaga
     8941 cgccttcgtc acggtgctac ctttgaaaac agcggaacag ctccgggctc agttcgggga
     9001 tttcttccac tgcaattcgc ccggggcggg tcaggccatg cggagcatgc tgagcgtcat
     9061 cctggtatcc gacctcgaag ccgtcaagga gaaggtctcc cgggcgatgt cgctggtgag
     9121 gaagtcccag atccccgtgg tccgcttccg ggggttccgg gtccatgtga cccggcagac
     9181 ctacgccaag atggaggttg tggaccatac gggcacaacc ctcttctacc tgaacgattt
     9241 cgagatcctc aaatccaact atctgcagta acggtccagg aacggtctat tttgcgaagg
     9301 ccttgagaca cacgttcgct ttcagaaaat cgatctggtc cgaagaatcc gtgcgcgttg
     9361 tcaggtcgct ccatcccttc ttcttcgggt ctgcgctcac gaagctctgt cccgccatgg
     9421 cgttggcccc ggcggcaaaa ctgttgggag acgctcccac ccggatggga cactcgacgg
     9481 cgatgggttc ggtggagttg ctcgtagtga gtctgacgac caccgagaat ttcttgaagg
     9541 gcgtgattgg tacccgtttg ggcagtttca cggtgtgata acccgccaat ggcatggttt
     9601 gccgggtcag ggcaaagacc cttttcccgt ccgtggggct gttcttcttg atccggtcat
     9661 aatcaacggc caccctgtcg tagacgtaga tgtcgtattc acaatccttt tcaggagtgt
     9721 agaaaccgac ggcggaaatc cgtgatcctt ccgggctccc ctggaaaatg ttggccatcc
     9781 aggcagtatg gctgttgaac ccgatggaga cgacccaccc ctgggggtcg tattgatagg
     9841 ccattttgta gtttgcggcg gattcggttc cgccgaacaa agtgagatcc cttacgttga
     9901 tgtcatagta agacaggtag aaatacccgc ggtctcccca accctctccc cagctgttct
     9961 tcacgataaa tgccccgttg ccggggggca tcgtcttgaa gttgctcctg tcgtagtggt
    10021 cgtcccaacc cacaatggcc actccatggt tgttctcctc gatatcgggg caatagtacg
    10081 atgatttctc ggagttgtag taagcccctt tcacatagaa ggacatggca acggccccgt
    10141 ggttgaagac ggcccacttg atggccccgt tgtccgtggg accttctcgg ttgggcagaa
    10201 agaggacctc ttgcagatgc ttcttcaccg agggatcgga agcatgctgg gaggattggg
    10261 tgccggcgaa aggatacggg taagtagcct ccgcaaaggg gccgctccac cgggcgaggt
    10321 atgccgagga catgaagaag gtgcctcctt cacagatccg atcgtcgaac ccgtgattct
    10381 ggttcaaatc ggcttcggag aaatcccact tctccccgtc tttcaggata caggattcca
    10441 gcgaggccat cgtgccgaag gcccagcagg agccgcaacg gccctggtcc cgaacggggg
    10501 tcactctctt cacggcccgc aggtcgaatt tgggcgggaa gagcgcggcc gaccccatat
    10561 gaggagacag agggccccga ttcaccgagg ataggtccac agggggaggc acgtacccga
    10621 ggaagcgacc ggcgaaggtc cgggactcga gtccgcctga agccgatttc cgaacataat
    10681 ccacaaaggc ggcgtttagc ggcgccgccc tcagttcgga atccacgtcc ggttgggccg
    10741 cagcgggcga cggaagcagc agctttccca acaagaatgc gaagaacgcc gcggtacaaa
    10801 caacgaacgg cttccgaact ttcggggtct tcatgttgtc tcctctccat tgggttggtt
    10861 tccgccgatg tggcccttcc ccatttccgg acggggacaa cttcgattcc aatacccgcc
    10921 ccaatctctc ccacccggga accgacccgg ttctcccgtt gccgaattcc ccctcttttc
    10981 gtttccaaac tcggctaagg aagaaaaaga gaggggcccg cctgctgaaa cgacgggagt
    11041 cccgaagact ctatcacgaa gaatgaacgg agtgaaagcc ttaaggggga agaagccggg
    11101 ggcggaatga aagtccaccg cttcgaggtc tgccgtccat gctgatccgt cgggacagag
    11161 tcggtactcc atctgcgggg attgccgccg ttgggcatca ttccgtgctt tggaatgccg
    11221 aggcggacgg caggcccccg gacttgccgc catggcgcga gactcgagag gagatcgcca
    11281 gggaaagacg gcatacaatt tgctataaga ctgtgccgag agaaacccgg gagcgggaat
    11341 cgagggacgc aaaagggcca gtggattcca ccgcccgatc cccggctccg gcctccggga
    11401 tgagcgttcc cgtgccgagt tgcggcccgc cgagtctgcc tgtgccgccg caaaccgatc
    11461 ttcaggaggt ggaagagtcg acgcagcccc cttccatgcc tttcaaggag ccccaggatg
    11521 ttgtcgctgg atatgaaaac cattgtcttc agctatctgg ccaactcgac ggtctgcctg
    11581 ttggtcctgt tccttttgtg gcgtcagagc cggaagcgtt ttgacggcat aggcttctgg
    11641 gtgctcgatt acttgtttca gacggcgggg ctggctctca ttgtgctgcg cggcagcatt
    11701 cacgagtggc tgtcggtggt ggtggccaac accttgatcg ttggaggcgc cctcctgggg
    11761 tatatcggac tggaacggtt tgttgggaaa aggagctccc aggtgcgaaa catcctggtg
    11821 ctgggggcct tcgtgacgat ccacgcctac tttgcttttg tccagcccaa cctggccgcg
    11881 cgaaacctca acctttccct gggattgctg atcctgtgcg gtcagtgtgc gtggctcctg
    11941 ctgtacggag tggaacccgg catgcggtcg ttgacccggc tggtgggcgt agtgtttggc
    12001 ctctactcgc tgctcagcgt cgtgcggatt gtggaatcct tggtcgggcc gaacccgggt
    12061 acggacttct tccggtcggg cgccttcgat cgctcggtgc tgctttccta ccaggtcctt
    12121 ttccttcttc tcacctacag cctggtgctc atggtcaaca agcgcctgtt gggggacatc
    12181 aagatccagg aggagaagtt tgccaaggcg ttccgtcatt ctcccaacgc catcaccctt
    12241 actcggcttt tcgacggaac gatcatggaa gtcaacgaag gcttctcgaa gatcaccggc
    12301 tacgagtatg gggaggccat cgggaagacc accgtggacc tgcatctttg gaatagcgag
    12361 agagaccggc tgagcgcggt ggaagagctc gccaagaccg gcaggatcca ggacagggaa
    12421 ctgcagcttc gcaaaaagtc gggggaaatc gtcaccggcc tcttatcggc agaggtcttc
    12481 cgcatgaacc accgggaatt catcctgtcc agcctcacgg atatcagtga tcgcaaacga
    12541 gccgaagccg aacgggaaga gcacatccgg gaactgcggc aagccatggc ccgggtgaag
    12601 caattgagcg gcctgctgcc gatttgcgcc tcctgcaaaa agatcaggga cgaccaaggt
    12661 tactggacgc aaatcgaggc ctacattcgg gaccgttcgg aggcggaatt cagccacggc
    12721 atctgtccgg agtgcgccaa aaagctatac cccgagatat ccgggagggt gctctccaaa
    12781 ggaacgaccg acgaccgtgg gcagtaatgg tgggggggga gagggccgtt gcttcccggg
    12841 aggtctcggg gatttgcgga tgtacgggtc tgtcgtgacc tcgtgaacgc cccagctcag
    12901 gtagtgccgg ggattagcgg acgtacggat tttgccccca agggccccga aacggtcgat
    12961 gtcgaggttt tgcagggcgg ccacgatgaa accacggata ccggggcaac gacccgctgg
    13021 ttcggcgagc ccgccgggga ccgtttcggg ggaaggccgc agcggcggga caaagtccca
    13081 gtgacgccgg aaggcctgcc ggggatcaac caaggtctga tggttgcgaa accgcaggtt
    13141 ggcaagcgac ggccggccga aaaccaacgg ctcatccggc agatgagtac cccggggttg
    13201 aaaaaaggga atggaaacgg caaggcccca acttgtttga cacccatcga acaggaaggg
    13261 agaacaagcc gtgtccacgt c
//
LOCUS       DAOOVC010000105        12820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3224_length_12820_cov_6.603134, whole genome shotgun sequence.
ACCESSION   DAOOVC010000105 DAOOVC010000000
VERSION     DAOOVC010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12820
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3224_length_12820_cov_6.603134"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PLM79_12645"
     CDS             <1..485
                     /locus_tag="PLM79_12645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV87207.1"
                     /translation="EQQVYWVPTLFTMKAYASLLEPGSLESRVAALNLESQLEQVSRA
                     RAMGVLLAVGTDSGSLGVHHGTSFVEELKLLQEAGLDLHEILRAATLHGAALLGVEDE
                     CGPLRVGSPATFVALLGPPSRLIESLSRPTVVCIRGVDVLRGSMPIPGPRPGPLCSFP
                     "
     gene            702..2633
                     /gene="ftsH"
                     /locus_tag="PLM79_12650"
     CDS             702..2633
                     /gene="ftsH"
                     /locus_tag="PLM79_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOV87208.1"
                     /translation="MNPFYKNLALWLVISLMVILLFNMFHQPQPSTQETTYSQFLNSV
                     QKREVTSVTIQGNHVLGTYVNGKSFRTFAPRDAELIRNLRDHGVEIQAKPEEESPWYM
                     TILISWFPMLLLIGVWIFFMRQMQMGGGKAMSFGKSRARLLNESSKKVMFDDVAGVDE
                     AKEELQEVVEFLKDPKKFTRLGGRIPKGVLLVGAPGTGKTLLARAIAGEAGVPFFTIS
                     GSDFVEMFVGVGASRVRDLFMQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRQVVVPVPDIRGREGILKV
                     HTSRKPLSSDVDVKTLAKGTPGFSGADLENMVNEAALLAARKNKDFIDMADFEAAKDK
                     VMMGLERKSMILSEEEKRTTAYHEAGHALVARLLPGADPLHKVTIIPRGRALGLTQQL
                     PQDDRHTYSREYLLDNISILMGGRVAEELVFDQRTTGAANDIERATQLARRMVCEWGM
                     SDSLGPLSFGKREEQIFLGREIAQHRDFSEHTAVEIDREVRRIVEENYQRARETVKDH
                     LDLLHGIAHALLERETLDLRDIDAIISETRPDLVEKTAEPAGGSASPARAQKTPGDSK
                     EAVSGSSPPASPTEADPES"
     gene            2699..3580
                     /gene="folP"
                     /locus_tag="PLM79_12655"
     CDS             2699..3580
                     /gene="folP"
                     /locus_tag="PLM79_12655"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOV87209.1"
                     /translation="MSVSPRKTFRLELGDRVLELGSRTLIMGILNVTPDSFSDGGRFS
                     DFPRAVEHARELIAAGADILDVGGESTRPDADGAPLDIELDRVIPVIESVRKFSDVPI
                     SIDTTKAEVARQALRAGANLVNDVSALRFDNRMTDVLAATKAPVILMHMQGSPKTMQK
                     QPFYHSLLSEILAFLEERIQYAVDHGVSRSQILVDPGIGFGKNVSHNLRLIRDLDLLH
                     CLDRPILLGASRKRFIGAVLDKSVDDREVGTAVANSFAIHAGVHILRVHDVAYHRQVV
                     RMGDALRSCPDFGETTG"
     gene            3715..>4091
                     /locus_tag="PLM79_12660"
     CDS             3715..>4091
                     /locus_tag="PLM79_12660"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOV87210.1"
                     /translation="MEKGTFKMLLAMDIGNTNTVLGLFRDDEIVQDWRIRTEVNATVD
                     EFGILIRNLFAFAELPMDSVKHVIISCVVPPVLNTADRFCRKYFGVSPFIVGPGIKTG
                     MPIFYDNPKEVGADRIVNAVAAY"
     assembly_gap    4092..4191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4192..4386
                     /locus_tag="PLM79_12665"
     CDS             <4192..4386
                     /locus_tag="PLM79_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantothenate kinase"
                     /protein_id="HOV87211.1"
                     /translation="YGYAALVDGIIQRIKREVHVETRVIATGGLATLIAGECPAIEEV
                     DDFLTLKGLKIIFERNRKGP"
     gene            4383..5276
                     /locus_tag="PLM79_12670"
     CDS             4383..5276
                     /locus_tag="PLM79_12670"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOV87212.1"
                     /translation="MKPRALQPGDLLALVAPSSPFDPDRFEQGSRILEDLGYRLLPGN
                     HIHKRRGYLAGSESERAQDLMRAFAQPDVSAVVCIRGGYGSSRLLPWLSFPRLGGQPK
                     IFLGFSDATFLHMAFQTRLKWVTFHGPNLMNLAEHPEFAADFQKALSGKQDFTWSFGE
                     DNVLRHGTGRGRLMGGNLTCMAHLLGTPYFPDLEGAVLMIEDCSEALYRLDRLFNHLK
                     LAGKIEGLAGLVLGQFKDCCTCGQIRDMILESVNTCSFPVVAGLPFGHGLQNRIIPLG
                     VPFSLNTYEGKLQALENPLVL"
     gene            5254..6468
                     /locus_tag="PLM79_12675"
     CDS             5254..6468
                     /locus_tag="PLM79_12675"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOV87213.1"
                     /translation="MKTLSSSEHPSLGRLDRLFCEALSQKVFSGASLLIADGSRILLE
                     QSWGKTRVDGPCVDPQTHFDLASLTKPLFTTPLCMISLGMGKFRLEDTLDRFWASNVS
                     REKTAITIRHLLSHSSGLPAYKPFYSALLKIPAPQRRTMLLRWILETPLLHPPGKAAV
                     YSDLGYILLGILLETVWEQSLDRLLERMLRSFRSGTPWTREANGSESSAEELSPADEV
                     AFAPLQVFPDPELPPVRMGKKRLSYAATEHCPWRRRLLEGEVHDENAYCLGGIAAHAG
                     LFGTPRNVRTVLDFLWKARSTAPAHIPWLRDVVGEFWERQRWVPQSTWALGFDTPTPG
                     QSSAGSRFSHLSVGHLGYTGTSFWLDPDRELLVVLLSNRVYPSRQNDKIRAFRPKLHD
                     LAVDVYEELQRV"
     gene            6446..7876
                     /locus_tag="PLM79_12680"
     CDS             6446..7876
                     /locus_tag="PLM79_12680"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOV87214.1"
                     /translation="MKSSNEYKTIYLMGIGGIAMGTLAGMLKDSGYEVLGSDQNLYPP
                     MSTHLESLGIPLFQGYRAENLRVRSVDLVIVGNVIRRENPEAQAVLEDGIPHMSMPQA
                     IAHFFLGDHQSMVVAGTHGKSTTSSLLGWILAQGGMDPSVFVGAFLNNWAASYRLGKG
                     PFMVLEGDEYDTAFFDKGPKFLHYRPKLGIITSVEFDHADIFPNLESIVEAFRAFVRL
                     IPSDGHLILNGDDPICRMLARECGGSVVTYGRSEHADWRILDRDCDQGRASCVYFDPV
                     RGERRKLVTSLMGRHNLGNALAAVVAATLAGVSEHDIRESLVSFRGVKRRQDWVGNVR
                     GIRVMDDFAHHPTAVSETLLAVRECHPEARLIAAFEPRTNSSRRKVFQEDYATAFKVA
                     DVICVKSPPGLESIPEPERLDAAKLVRDIARGGRDAHLFEGTDLLIRFLSDFCGPGDL
                     VVCMSNGSFDGLPHRLTEALRNRTPV"
     gene            7900..8463
                     /locus_tag="PLM79_12685"
     CDS             7900..8463
                     /locus_tag="PLM79_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOV87215.1"
                     /translation="MPRILLHSCCGPCTLYPLQKLREGNWVVHPFFYNPFIQPYQELS
                     RRIEAFEQVVEKTSARAILRKDYDLEGFFREVVFRESRRCHYCYSVRLNAAARTAKKS
                     RMDAFTTTLLYSKHQNHELICSIAQEASIRNGIPFHYEDFRLGRAEGQSRARSLGIYR
                     QQYCGCIYSEKERFTPRDDPGPEEGKE"
     gene            complement(8526..8810)
                     /locus_tag="PLM79_12690"
     CDS             complement(8526..8810)
                     /locus_tag="PLM79_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV87216.1"
                     /translation="MAERKKDFTHAHSTLCKGEGGRTGDWRSVRPRIDFSRCTPCKNN
                     RPSCFICWLYCPEAVVKRTIPVEIDLEYCKGCGICAEECPAKAIEMVEEP"
     gene            complement(8857..9450)
                     /locus_tag="PLM79_12695"
     CDS             complement(8857..9450)
                     /locus_tag="PLM79_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV87217.1"
                     /translation="MKRRRIDVRWHGRGGQGAVTAAMILAEAAYYEGYRGVTAAPFFG
                     AERRGAPVIATTRFGREPIRTFSLVEYPDIVVVLDETLLKVVSVAAGVRPDGLVLVNS
                     PRPPEEIHLDAPLNVATTDAGEWAKRAGLVVAGNVIVNTSILGGVAGATGLISLENLE
                     KALRDHFGEEAGDRNVQAARSAYSGVRMLKDFTLACA"
     gene            9642..10079
                     /locus_tag="PLM79_12700"
     CDS             9642..10079
                     /locus_tag="PLM79_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87218.1"
                     /translation="MSDQERPLRSVFLKNGLEVRFFDGSKKTAGDRWQVRLIMEVPLK
                     VEESYFSDLPNPREAFEGFVGEFTTRTFFRREKVRNFIDEKEVGSLLKSLMDESLDAG
                     ARYLESGEFPRRAILKQYFDWEKDRSWQRCYREHLEGASGAKK"
     gene            complement(10165..10716)
                     /locus_tag="PLM79_12705"
     CDS             complement(10165..10716)
                     /locus_tag="PLM79_12705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOV87219.1"
                     /translation="MKPFQKVAGSEGNLDSIPVRVNYLTPLDAINSPEVFVYKEKRRL
                     YIIQDQVLVRDYPVGLGFSPQGDKQLEGDGRTPEGKFFVCMKNSSSRFLKSLGLNYPD
                     KKHAERAFFSGVITPLEFRDILLAFENKATPPWNTVLGGKIFIHGGGAHKDWTEGCIA
                     LYNSDMEELFRIASVGTPVSIRP"
     gene            complement(10940..>12820)
                     /gene="pilQ"
                     /locus_tag="PLM79_12710"
     CDS             complement(10940..>12820)
                     /gene="pilQ"
                     /locus_tag="PLM79_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOV87220.1"
                     /translation="LVLLCCAGCTAPGITGKGAAADVQAADPGEKAGTSAAVVTGVGQ
                     KRTDSLLGIQVQDLADETIIRLAGNGAFHDYQFRRLGDDRFALELGDVTRKETLPTLP
                     TSSKRARLVYADTQATQGVQIIGTLKGPLDRYVLNDVGNDLLLTLYLAETPVAPATVS
                     AARTASADPAAKKSAVKPGGKAAAQAAAGKTAKPQTQTAKVQARTPEVPGTPETGDPL
                     RKRYTGKPISLDLLDADLRNVLRLIADITGSNIVIEPDVTGKVTLKVEQVPWDQVLDM
                     VLSMNDLGKEQLGNVIRVARQAKLKDEWKEQTQSLKAKQELIEATKDLGEISTAYLTV
                     NYASPADIAAKISEIKSEKGKISVDERTSLILYSDFPARINAARELLGRLDKATPQVL
                     IEARIVTMNSDLSRELGIDWKLQTSHITRSSTVPPFTQNFAVNSPPSSLFGFTWAQVI
                     GETLVNVDLKLSALETATQLKVMAAPRVLTINNVKAVITQGTQIPYLQLSESGAVAAT
                     QFKDAVLELQVTPHITPDQKVRLEIQAKQDEPTSVTGATQPGIETRKIATELLVDDGN
                     IVVIGGVIRNRDAYTRSATPGVHRIPIIGRLFKSESEETRRTEMLIFISPKIVEATRN
                     PV"
BASE COUNT         2510 a         3822 c         3704 g         2684 t
ORIGIN      
        1 cggagcaaca agtgtactgg gtccccaccc tttttaccat gaaagcttac gcatcgctcc
       61 ttgagccggg cagcctcgag agccgggttg ccgctctgaa cctcgagtcc cagttggaac
      121 aggtctcgag ggcacgggcc atgggggtcc ttctggccgt gggcaccgat agcggaagcc
      181 tgggcgtcca tcatgggact tcgtttgtgg aggaactcaa gctcctgcag gaagcggggc
      241 tggaccttca cgaaatcctt cgcgccgcaa ccctgcacgg agccgccttg ctgggcgtgg
      301 aagacgagtg cggacccctg cgcgtgggct cgcctgccac atttgtggcc ctcttgggcc
      361 ccccgtcccg gctgatcgag tccttgagcc gacccaccgt ggtctgtatt cggggagtgg
      421 acgtgttgcg gggttccatg ccgatccccg gcccccgacc cggtccgctt tgttccttcc
      481 cctaatggaa ggaatctgtt atagacctga gctgaaccgg cagcccaagg gtcattgccc
      541 gcagccaaag gccgaacgcc cgttcggcat gggatcaacc attgccgtcc atcggctcat
      601 gccgtatccc cgaagttggg tgcggatggg ccgtgtccgt gttggggcaa ccgttcagcc
      661 aagcatcttc tttggaaaag aagtctcgag gaggtccacg attgaatccg ttttacaaga
      721 acctagcgct gtggctggtc atcagcctca tggtgatcct gttgttcaac atgtttcatc
      781 agcctcagcc gtccacccag gagaccacat acagccagtt tttgaattcg gttcagaaac
      841 gggaagtcac cagcgtgacc atccagggaa atcacgtcct ggggacctac gtcaacggca
      901 agtctttcag gacctttgca cccagagatg cggagctcat tcgcaatctg cgggatcacg
      961 gcgtcgagat ccaggccaag ccggaagaag aatccccgtg gtacatgacc atcctcatca
     1021 gctggtttcc catgctcctg ctcatcggcg tctggatctt tttcatgcgc cagatgcaga
     1081 tgggcggcgg aaaggccatg agtttcggca agagccgggc gcggcttctc aacgagagct
     1141 ccaagaaggt catgtttgat gacgtggccg gggtggatga agccaaagag gaactccagg
     1201 aagtcgtgga gtttttgaaa gacccgaaaa aattcacccg cctgggaggc aggattccca
     1261 agggagtgct gctggtggga gctcccggaa cgggcaagac cctcctggcc cgggccatag
     1321 ccggggaggc cggcgttccc tttttcacca tcagcgggtc ggatttcgtg gaaatgtttg
     1381 tgggcgtcgg ggcctcccgg gtcagagacc tgttcatgca ggggaaaaag aacgcccctt
     1441 gcatcatctt catcgacgag atcgacgccg tgggtcgtca ccggggagca ggactgggag
     1501 gcgggcacga cgaacgggaa cagaccctca accagttgct ggtggaaatg gacggatttg
     1561 aatccaacga gggcgtgatc ctgattgccg ccaccaaccg tcccgatgta ctggacccag
     1621 ccctgctgcg cccgggaagg ttcgaccgcc aggtggtggt gcctgtcccg gacattcgcg
     1681 ggcgcgaagg catcctgaag gttcatacct cccgaaaacc tctttcttcc gacgtggatg
     1741 tcaagaccct tgccaagggg actccggggt tttccggcgc agacctggaa aacatggtca
     1801 atgaagccgc cctgttggca gcgaggaaaa acaaggattt catcgacatg gccgacttcg
     1861 aggccgccaa agacaaggtc atgatgggac ttgagcgcaa aagcatgatc ttgagcgagg
     1921 aagaaaagcg gaccaccgcc taccacgaag cgggacatgc cctggtggca aggctcctgc
     1981 cgggggcgga ccccctccac aaggtgacga tcattccgcg ggggcgggcc ctgggactca
     2041 cccaacagct gccccaggac gaccggcata cctattcccg ggaatacctg ctggacaaca
     2101 tttccatcct catgggaggc cgtgtggccg aggaattggt gttcgaccag cggaccacgg
     2161 gagccgccaa cgacatcgaa cgggcgactc agcttgcccg tcgcatggta tgcgaatggg
     2221 gaatgagcga ctcgttgggg ccgctcagtt tcggaaagag agaggaacag atcttcctgg
     2281 gacgggaaat tgctcagcac cgggacttta gtgagcacac cgccgttgaa atcgaccggg
     2341 aagtgcgccg catcgtggag gagaactacc agcgggcccg ggagaccgtc aaggaccacc
     2401 ttgacctgtt gcatgggatt gctcatgcac tcctcgagag agaaactttg gaccttcggg
     2461 acatcgacgc catcatttcg gaaaccaggc ccgatttggt ggaaaagacc gccgagcccg
     2521 ccggagggtc cgcatccccg gcccgggctc aaaaaacccc aggagattca aaggaggccg
     2581 tctccgggtc ctccccgccc gcctccccca cggaagcgga tcccgagtct taagccctcg
     2641 gatggaaggg ccggggccgg atcgagtttg cgctgaaaac ctctgaaacg agagtcccat
     2701 gagtgtttct cctcgaaaga cattccgcct ggagttgggc gaccgggtct tggagctggg
     2761 ttcccggacc ctgatcatgg ggatcctcaa cgtgaccccg gattcctttt ccgacggggg
     2821 ccggttctcc gattttcccc gggccgtcga gcatgctcgg gaattgatcg ctgcgggagc
     2881 ggacatcctg gacgtcggag gggaatccac gaggcccgac gccgacggcg cccccctgga
     2941 catcgagctg gaccgggtca tccccgtcat tgaaagcgtt cggaaattct ccgacgtccc
     3001 catctccatc gacaccacca aggccgaagt ggccaggcaa gcgctccgag cgggtgcgaa
     3061 cctggtcaac gatgtgagcg ccctgcgatt cgacaatcgc atgaccgacg tcctggcagc
     3121 caccaaggcc cccgtgatcc tcatgcacat gcagggctcc cccaaaacca tgcagaaaca
     3181 gcctttttac cactcactgc tttcggaaat cctcgccttt ctggaagaac gcatccagta
     3241 cgccgtcgac cacggcgtga gccgcagtca aatcctcgtg gacccgggga tcggtttcgg
     3301 caagaacgtc agccacaacc tgcggctcat ccgggacctc gatcttctcc actgcctgga
     3361 ccggcccatt ctgctgggag cttcacgcaa gcgcttcatc ggcgccgtcc tggacaaatc
     3421 cgtcgatgat cgagaggtgg ggacggccgt ggccaacagt tttgccatcc atgcgggggt
     3481 ccacatcctt cgggtccacg acgtcgccta ccaccgacag gtggttagga tgggagacgc
     3541 ccttcgcagc tgtccggatt tcggggagac gacaggctga tccccgggca tcgggttccg
     3601 cgagaagggt ccccacgccg atccgtcgag gggtgatttc cgactcccga agggggactg
     3661 tctcgaccag gtccggcaag ggaccggccc cgttttggaa attcaccgaa aagaatggaa
     3721 aagggaacct tcaagatgct cctcgccatg gacatcggaa ataccaatac ggttttgggt
     3781 ctgttccgag acgatgagat cgtgcaggat tggcgaattc gaaccgaggt caatgctacg
     3841 gtggacgaat tcgggatcct cattcgtaac ctctttgctt tcgcagagct tcccatggat
     3901 agcgtgaagc atgtgatcat ctcctgcgtg gtgccccccg ttctgaatac ggcggaccgc
     3961 ttctgccgca aatacttcgg tgtgtccccc ttcatcgtgg gtccggggat caagaccggg
     4021 atgcccatct tttacgacaa tcccaaggag gtgggggccg acaggatcgt gaacgccgtg
     4081 gcggcctatg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntacggttat
     4201 gccgctctgg tggacggaat cattcagcgg ataaagcggg aagtccatgt ggagacccgg
     4261 gtgattgcca ccggaggact ggcaacactc attgccgggg aatgccccgc catcgaggaa
     4321 gtggacgatt tcctgaccct caagggcctc aagatcattt tcgagaggaa tcgaaaggga
     4381 ccatgaagcc tcgagccctg caaccgggag acctcctcgc cctggtggct ccctccagcc
     4441 ccttcgatcc ggaccgattc gagcagggaa gccgaattct ggaggacctt ggctatcgac
     4501 tcctgccggg aaatcatatc cacaagcgcc gggggtacct ggccggcagt gaatccgagc
     4561 gtgcccagga cctcatgagg gcctttgcgc aaccggacgt ttccgcagtg gtttgcatcc
     4621 gcgggggata cggaagttcc cgcctccttc cctggctctc ctttccccgc cttgggggtc
     4681 agccgaagat tttcctgggc ttcagcgatg cgacttttct gcatatggcc tttcaaactc
     4741 gcctgaaatg ggtcaccttt catggaccca atctcatgaa ccttgccgaa caccccgagt
     4801 ttgccgccga ttttcagaaa gccctgtcgg gaaagcaaga cttcacctgg tctttcggag
     4861 aagacaacgt ccttcgccac ggaacggggc gcggcaggtt gatgggagga aaccttacgt
     4921 gcatggccca cttgctggga acgccctatt tccccgacct ggagggcgcc gtcctcatga
     4981 tcgaggattg ctccgaggct ctctatcgcc tggatcgtct cttcaaccat ttgaagctcg
     5041 ccgggaagat cgagggactt gccggtcttg ttctggggca gttcaaggac tgctgcacct
     5101 gcggacagat ccgggacatg atcctggaat ccgtgaacac ctgctcgttt cccgttgtgg
     5161 ccggtttgcc ctttgggcac ggactccaaa accgcatcat ccccctgggc gtcccctttt
     5221 ccttgaacac ttacgaagga aaactccagg cccttgaaaa ccctctcgtc ctctgaacat
     5281 ccctccctcg ggaggctgga ccggcttttt tgcgaggccc tcagccagaa agtcttctcg
     5341 ggagcgagtc ttttgatcgc cgacgggtcc cggatcctct tggagcagtc ctggggaaag
     5401 acccgtgtcg acggtccttg cgttgaccct cagacccatt tcgatcttgc gtccctcacc
     5461 aagcctttat ttacgacacc cctttgcatg atatccctgg ggatgggcaa gttccgcctg
     5521 gaagacaccc tggatcgctt ctgggcttcc aacgtttccc gcgagaagac cgcgatcacc
     5581 atccgacacc ttctgagcca ttcttcgggg cttcccgcct acaagccttt ttattcagcc
     5641 ctcttgaaga tcccggctcc acaacgccgg accatgttgc tgcggtggat tctcgagacg
     5701 cccctcctcc atccccccgg gaaggctgcc gtgtacagcg acctgggtta catcctgctg
     5761 gggatcctcc tggaaactgt ttgggagcag agcctggatc ggctgctgga gcgaatgctt
     5821 cgatccttcc gatccggtac cccttggacc cgggaggcaa acggctccga gagctctgcc
     5881 gaagagttgt cccctgccga cgaggtcgcc tttgccccct tgcaggtatt tccggacccc
     5941 gagttgcccc ccgtaaggat ggggaagaag aggctttcct atgcggcaac cgagcactgt
     6001 ccatggcgcc gacgtctcct ggaaggggag gttcacgatg aaaacgccta ttgtctcggg
     6061 ggaatcgcgg cccatgccgg cctttttggc acccctcgaa acgtccggac cgtcctggat
     6121 ttcctgtgga aggctcgttc gactgctccc gcccatattc cgtggcttcg ggacgtggtc
     6181 ggagagttct gggaaagaca acggtgggtg ccccaaagca cctgggcgct gggattcgac
     6241 acccctaccc cgggacaatc cagtgcgggc agccgtttca gccacctgag tgtcggtcac
     6301 ctgggctata cggggacctc tttctggctg gatccggatc gggaacttct cgtggtgttg
     6361 ctctcaaacc gcgtgtatcc ttctcggcaa aatgataaga ttcgggcctt tcgtccgaaa
     6421 ctccatgact tggcggtgga tgtgtatgaa gagctccaac gagtataaga cgatctatct
     6481 catgggcatt ggcggaatcg ccatgggaac cctggcgggc atgctcaagg acagcggcta
     6541 cgaagtactc gggtcggatc aaaacctcta tccgcccatg agcactcacc tggagagtct
     6601 cgggatcccc cttttccagg gatatcgagc cgaaaacctc cgggttcgtt ccgtggacct
     6661 ggtcatcgtg ggaaacgtca tccgccggga aaatcccgag gcgcaagcgg tcctggaaga
     6721 cggcattcct cacatgtcca tgccccaggc catcgcccac ttcttcctcg gggatcacca
     6781 aagcatggtg gtggccggaa cccatggaaa gagcaccact tcttctcttc tcgggtggat
     6841 tctagcccag gggggaatgg accccagcgt ctttgtcgga gccttcctga acaactgggc
     6901 agccagctac cggttgggaa agggaccctt catggttctc gaaggcgacg aatacgacac
     6961 cgcctttttt gacaagggcc ccaagtttct tcactacaga cccaagctgg gcatcatcac
     7021 cagcgtggaa ttcgatcacg cggacatctt ccccaacctc gaaagcatcg tggaagcctt
     7081 tcgggccttc gtacgattga tcccctccga cggacatctc atcctgaacg gagacgaccc
     7141 catctgtcga atgttggcac gggaatgcgg gggtagcgtc gtcacctacg gcaggtccga
     7201 acacgcggat tggcgtatcc tcgatcgcga ctgcgaccaa gggcgggcgt cctgcgtcta
     7261 tttcgacccc gtacgggggg aacgacgcaa attggtgacg agtctcatgg ggcgccacaa
     7321 tctgggcaat gccctggcgg ccgtggtcgc cgccactctg gccggagtga gcgaacacga
     7381 cattcgggaa tccctggtct cttttcgagg cgtcaaacgc cgccaggatt gggtcggcaa
     7441 cgtccgggga atccgcgtga tggatgactt tgcccatcat cccacggcgg tttcggagac
     7501 ccttctggcc gtgagggaat gtcacccgga ggcccggctc attgcagcct ttgaaccccg
     7561 caccaactcc agcaggcgaa aggtctttca ggaggactac gccacggcct ttaaggtcgc
     7621 ggacgtgatc tgcgtcaaat cccccccggg actcgagagc attcccgaac ccgaacgcct
     7681 cgatgcggcg aaactggtcc gagacattgc ccggggcggc cgggacgcgc acctcttcga
     7741 gggcaccgat ctcctcatac gcttcctttc cgacttttgc ggtccggggg atttggtggt
     7801 ctgcatgagc aacgggagtt ttgacggtct tccccatcga ctgacggagg ccttgagaaa
     7861 tcgcacgccc gtttgatttc tcggcccgga atcgatctca tgccaagaat cctcctccac
     7921 agctgttgcg gcccatgcac cctctatcct ttacagaaat tgcgcgaggg gaactgggtg
     7981 gtgcaccctt tcttctataa ccccttcatc cagccctacc aggagctatc gagaaggatc
     8041 gaggcctttg aacaggtggt cgagaagacc tccgcccggg ccatcctgcg caaagactat
     8101 gatctcgaag gtttcttccg ggaggtggtg tttcgggaga gtcgtcgctg ccactactgc
     8161 tattccgttc gtttgaatgc agcggcccgc acggcaaaaa aaagccgcat ggatgcgttc
     8221 accaccactc tgctttacag caagcatcaa aatcatgaac tcatttgcag cattgcccag
     8281 gaggcttcca ttcgaaacgg aatccccttc cattatgagg atttccggct gggccgggcc
     8341 gagggacaat cccgggcaag gtcgctgggg atctaccgac agcagtactg cgggtgcatc
     8401 tacagcgaaa aggaacgctt cacgccccgg gacgaccccg ggccggaaga agggaaggaa
     8461 tgaggaggga agcaggttcc ttgggaggaa aggtccggag gccccccacc ggacgagggc
     8521 aatccctagg gttcttccac catctcgatg gccttggcgg gacactcttc ggcacagatc
     8581 ccgcagccct tgcagtattc caggtcaatt tccacgggaa tcgttctttt taccaccgct
     8641 tcgggacaat acagccagca aatgaaacag gaagggcggt tgtttttgca gggtgtgcac
     8701 ctggagaaat caatccgggg cctgaccgat cgccagtccc ccgttctccc tccttccccc
     8761 ttgcagaggg tggagtgggc gtgggtaaaa tcctttttcc tttccgccat gattcgtcct
     8821 tatctcccag gcaggcggtg ctgccaactc atcgggtcaa gcgcacgcca gagtgaaatc
     8881 cttgagcatc cgcaccccgg aatatgccga gcgggccgcc tgcacgttcc ggtctccggc
     8941 ctcttccccg aaatgatccc gcagggcctt ctccaggttt tccaagctga tcagccccgt
     9001 ggccccggca acccccccca gaatggaggt gttgacgatg acgtttccgg ccaccaccaa
     9061 cccggcccgt ttcgcccatt cgcctgcatc cgtggtagcg acgttcaacg gcgcatccag
     9121 gtggatctct tcgggaggcc tgggactgtt gacgaggacc agcccgtcgg gtcggactcc
     9181 cgcggcgacg gacacgacct tcaataaggt ctcgtcgagg accacaacga tgtccggata
     9241 ttccaccagg gaaaaggtcc ggatgggttc ccgcccgaag cgcgtcgtgg caatgaccgg
     9301 cgcacctctg cgctccgctc caaaaaacgg agccgcggta actccccggt atccctcgta
     9361 ataggcagct tcggccagga tcatggccgc agtaacggca ccttgtccac ccctgccgtg
     9421 ccatcgtaca tcaattcgtc tgcgcttcat ccggttccca ctcttttgga tcgtcccacg
     9481 gcggtcgaaa gcaccccaat tctttggtgc ctgtttaata ccgctttgat ggggtggagt
     9541 caacaggatg cgcccgaatt gtccgatctt gaacttttcg atccgtttgg taatagattt
     9601 tctgcgtttg gcaatcagga caacctttgg gagggcggga gatgagtgac caagaacgac
     9661 ctttgagaag cgtgttcctg aaaaacggtc tcgaggtgcg ctttttcgac ggatcgaaaa
     9721 agaccgccgg ggaccgatgg caagtccgac taatcatgga agtccctctg aaggtcgaag
     9781 agtcctactt ttccgacttg ccgaacccca gagaggcctt cgagggtttt gtgggcgagt
     9841 tcacaacccg gaccttcttt cgccgggaga aggtgcgcaa cttcatcgat gaaaaggagg
     9901 tggggagcct cttgaaatcc ctcatggacg aatccttgga tgccggtgcg cgttacctgg
     9961 agtccgggga gtttcccaga agggcgattc tgaagcagta tttcgattgg gaaaaggatc
    10021 ggtcctggca gcgttgctac cgggagcact tggagggagc gagcggggca aaaaaatagc
    10081 gggcggatag gttccgcccg ctctgagggc ccatttttcc ggaccgtgaa ggtcgccgat
    10141 ttttccttgg cgggacccgc gcacctatgg gcgaatggag acgggagtcc ccaccgaggc
    10201 aatccggaaa agctcttcca tatcgctgtt atagagggcg atgcagcctt cggtccagtc
    10261 tttgtgtgcg cctcctccgt gaatgaagat ctttcccccc agcaccgtgt tccacggagg
    10321 ggttgccttg ttttcgaagg caaggaggat atcgcgaaat tccaggggtg tgatcacccc
    10381 ggagaagaag gctctctccg cgtgcttttt atcaggataa ttcaacccca gggatttcag
    10441 aaatcggctg gagctgttct tcatacagac aaagaacttg ccctcgggag ttcttccatc
    10501 cccctccagc tgcttatccc cctgggggct gaaccccagg cccaccggat aatcccgaac
    10561 cagcacctgg tcttgaatga tgtaaagccg tcgtttctct ttataaacaa aaacttccgg
    10621 gctgttgatg gcgtccaagg gggtgaggta attcaccctc acgggaatcg agtctaaatt
    10681 cccctccgag cccgcaacct tttgaaaggg tttcacgcct ttttccgtac gcgcggaatc
    10741 cagagtggat cccgatttct gcatggaaca accgctcagg acgaaaaaca gacccaccag
    10801 accccacaga atgcagcgtc tacttgacga aacacccatc ctcatcctca acgttcccct
    10861 cttgccacaa tcccttggta caagcgactt ttcgtatccg gctccccctc gacggagccc
    10921 tttgagtgaa gcttttattc tacacgggat tcctcgtggc ttccactatt tttggactga
    10981 tgaagatcaa catttcggtc ctgcgcgttt cctcggattc cgacttgaac agccgcccga
    11041 tgatgggaat tctgtggacc cccggggtgg ccgaccgagt gtaagcatct cggttgcgaa
    11101 tcactccccc gatgaccacg atgttgccgt cgtccaccag caattccgtt gcaatcttgc
    11161 gggtttcgat ccccggctgg gtcgcccccg tgacgctggt tggttcgtcc tgcttggcct
    11221 ggatctcgag tctcactttc tggtcggggg tgatgtgcgg agtgacttga agctccaaaa
    11281 cggcgtcctt gaactgggtg gcggccaccg ctcccgattc gctcaattgc aggtagggga
    11341 tctgggtgcc ttgggtaatg acggctttga cgttgttgat ggtcaggact cggggggccg
    11401 ccatgacctt gagctgggtg gcggtttcga gggccgaaag cttcaggtcc acgttcacca
    11461 gggtttcccc gatcacctgg gcccaggtga atccgaaaag gctggatgga ggactgttga
    11521 cggcgaagtt ctgagtaaac ggaggaacag tgctggaccg ggtgatatgg ctggtctgca
    11581 gcttccagtc gatcccgagt tcgcggctca ggtccgagtt catggtcacg attcgggcct
    11641 cgatcagcac ctggggagtg gctttgtcca accggcccag gagttccctg gcggcattga
    11701 ttcgagcggg aaaatccgaa tacaggatca ggctggtccg ttcatcgacg gagatcttcc
    11761 ctttctcgct cttgatttcg ctgatcttgg cggcaatgtc tgcgggagag gcgtaattga
    11821 ccgtaagata ggccgtcgag atttcgccca ggtctttggt cgcttcaatg agttcctgtt
    11881 tggccttgag gctttgggtt tgctctttcc actcgtcttt cagcttcgcc tgacgggcga
    11941 cgcgaatcac gtttcccagt tgttccttcc ccaggtcgtt catggacaga accatgtcca
    12001 ggacctgatc ccaggggacc tgttccactt ttagggttac cttccccgtc acatcgggtt
    12061 cgatgacaat gttgcttccg gtaatgtccg caatgagccg aaggacgttg cgcaggtccg
    12121 cgtccagcag gtccaggctg atgggttttc cggtgtatcg ctttcttaga ggatcgcccg
    12181 tttccggagt tccgggaacc tcgggcgtcc gagcctgcac ctttgccgtc tgggtttggg
    12241 gcttggctgt ctttcccgcg gcggcctgtg cggccgcctt gcccccgggt ttcaccgcgg
    12301 acttcttggc tgccggatcg gcggaggccg tccgggccgc agaaacggtc gccggtgcga
    12361 ccggggtctc cgccaaatac agcgtgagaa gcaggtcgtt tcctacatca ttgagtacat
    12421 aacgatccag gggacctttg agggtcccga tgatttgaac gccttgggtt gcctgggtgt
    12481 cggcatagac cagtctggct cgtttggaag aagtcggcag ggtcggcagg gtctccttcc
    12541 gggtaacatc tcccagttcc agagcaaatc ggtcgtcccc caggcgtcgg aactgataat
    12601 cgtggaacgc cccgttgccg gccaaacgaa tgatcgtctc gtccgcaagg tcctgaacct
    12661 ggattcccaa aagactatcg gttcgtttct gcccgacccc cgtcacgacc gccgcggacg
    12721 tgcccgcctt ctcccccggg tccgctgctt gaacatccgc cgccgcgccc tttccggtaa
    12781 ttcccggagc cgtgcaaccc gcgcagcaca gaaggactag
//
LOCUS       DAOOVC010000106        12812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3230_length_12812_cov_6.483813, whole genome shotgun sequence.
ACCESSION   DAOOVC010000106 DAOOVC010000000
VERSION     DAOOVC010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_12812_cov_6.483813"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3206)
                     /locus_tag="PLM79_12715"
     CDS             complement(<1..3206)
                     /locus_tag="PLM79_12715"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOV87221.1"
                     /translation="MLRSILEVCWPRKDILEGTFNPEIFSAGLSQVMSAYRGGTSIAH
                     KLYTDADQFFGDGTYPTEGLRMVLADVFGRIAGQNPPAIHRLETAFGGGKTHLLIALV
                     HLGFRGRELASVTQNIVDSSLLPAAGEVSVVGIAGDEIPVHKPEGVRLVPYTLWGEIA
                     YQIGGEALYRTVEQEATSFAAPDAGFLDRILSGRKAIIMLDELAQYAARLEAARPNGG
                     EQLAAFLMVLNGYARTHRGISVVMTLASQSDAFGRQTERLRDLLSQVRGENVSEDEAL
                     GLAQRAEAASRSVVFRDASTVVPVRAGEISRILAVRLFDSCDPRAAVDAANAYMEMYA
                     KSGAALPERALRSDFRDVMASHYPFHPTFIDFLNTKLSTLETFQGTRGVLRVLALTIR
                     SIWGKKLKVPMIHSCHVDFRDSRTLNEIIGRTGSGDLLPVINADIGGPDTSSLDTGRS
                     QAELADRKNPHPAGHPLHEYTWKTVFLHSLVGRSQGLGSNLFGISEKDALFEIAFPGL
                     TPPQVETALREIKESAYYLRSDHGRYYASLEPSVNIVLAQIRRGISEPSVDQLLDTSV
                     RKLITGAGSSFRVIHDVSLPEHMPDNVNQPSIAVIAVNAKKVDGMDFLTTVGPNRPRI
                     QQNQLFLLVPSTVNVKGEVWNEDRVRRYMETLNRLKDLARWVLAMRRLKEKPENYGIH
                     ASKLNDPEFVSRQAERELALQTSLAQIYDTVWYPSASGQITHKEIKTGGGEGGFSILE
                     EMRRILQAEGELITADRAATLETLQALSGLFFQLDQTPALAAIEESFLCKRHWPVLEQ
                     STLLGTIIREGVNRGIWCLFRMGSAESTKPEVIYSTESGGIPLNLDLKAPGWSIVTVQ
                     GAKQREWIGETIDPAKIEQWVTSLAFEKKACTISDVVDEVRERHGEVPRDKITEAAEK
                     LIRSDKLYTYTGKPGQTEKPDTLVHGPPAIVHQAKSDDVIIVPSEASRRGWVTAKRNV
                     LNLSGTEALRAILPLLPRLGSLYSKGARSTVKSLDLSDLELPDGGRLRVSLEEVPPGS
                     MKKLGEFFEVLAGVIRQGGNTEVFLEIQEPDEDCLFIQA"
     gene            3485..3982
                     /locus_tag="PLM79_12720"
     CDS             3485..3982
                     /locus_tag="PLM79_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOV87222.1"
                     /translation="MSTEVFESAKETIGRAKTADELRAAQAVVLPILLGISDHAVGQV
                     IGRSRVSVVRMRKEFKQLHTPKGVAARNWGGRRSGYMTVDQEREFLSRFFNHGSQGSA
                     VNADEIKEAFEVLVGHKVAKTTIYRMLDRHGRRKITPRPHHPKSGAKARKSFTKTAKN
                     GDSMR"
     gene            4208..4540
                     /locus_tag="PLM79_12725"
     CDS             4208..4540
                     /locus_tag="PLM79_12725"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV87223.1"
                     /translation="MCSRHPDEFILMGLDGAGWHRAGNLRVPANMHLMFLPPYGPKLN
                     PVEPIWKSIRENRFPKAVFKNVDGVENQPEKALAALESGPDAVARLTGFPWIRWYQHA
                     RTLVLNAR"
     gene            complement(4640..4715)
                     /locus_tag="PLM79_12730"
     tRNA            complement(4640..4715)
                     /locus_tag="PLM79_12730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4680..4682),aa:Arg,seq:ccg)
     gene            complement(4819..5283)
                     /locus_tag="PLM79_12735"
     CDS             complement(4819..5283)
                     /locus_tag="PLM79_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87224.1"
                     /translation="MLRVKECMKVTIEFAIKTVSPSGEVTEVPPEVCSFVFGVDVQYP
                     SVETAIMNKCVGDRVQVYVPPEELFGHADEALIRELPRADYKQERLKPGKMYREMKKK
                     CLVQFLVKELKEDVIVADFNDPRAGTHAEFDILIKDIREATKEEMTPSCARP"
     gene            5437..6237
                     /gene="pgeF"
                     /locus_tag="PLM79_12740"
     CDS             5437..6237
                     /gene="pgeF"
                     /locus_tag="PLM79_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOV87225.1"
                     /translation="MTTRPEPCNPPSPSLERHVFPGLSRVPGLVHGIFKRHGGVSLPP
                     YAALNVAWDNGDNPRFVHENLSRIEKDLGLDCLAAGRQVHGDRILLVDENALGRAETR
                     GHLVITPPGDALVTRLRGVGLLIKIADCQAVFLVDPVQNVVANVHCGWRGSVQELPAK
                     VVRFLRDRLGCRPEDLLAAVSPSLGPCCAEFRNYRTELPASFAAYQAAPLHFDFWAVT
                     RRQLEEAGVRPDRIEIAQVCTVCESRDYFSYRGEKVTGRNAAVIGWQA"
     gene            6262..7131
                     /locus_tag="PLM79_12745"
     CDS             6262..7131
                     /locus_tag="PLM79_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOV87226.1"
                     /translation="MTSFQEDGRVVLHEKVSQQTYRLRIHSPRISAQALPGQFVMLQV
                     QKGIDPLLRRPFSFHRLLPGEDSFEILYRVVGRGTWVLSRTPPGAVLSILGPLGNGFL
                     IPSPQPSGVLIIAGGIGIAPLYELLIRLSDGRIPGREPPLRLLYGARTADELLPMSCF
                     HELGASVSFATDDGSLGYRGTVPELLGLLTAKEKLEPQLVYACGPLGMQYRVAVWVKE
                     RGIPSQLSLESLMACGLGACLGCALPAPHPDDPLRPHYVHVCKDGPIFEAGAIEWQKI
                     VGQQTIAPTFLFS"
     assembly_gap    7170..7179
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7180..8047
                     /locus_tag="PLM79_12750"
     CDS             <7180..8047
                     /locus_tag="PLM79_12750"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOV87227.1"
                     /translation="RELGAVVVKGISLEPLPGNPPPRLVETPGGLINAIGLENVGLEA
                     FLAQKLPYLEGMSVPVIVNIFGNTLEEYGELARRLDGVPGIVGLEINISCPNVKQGGM
                     VFGTDPSMAASVVGTVRKAASLPLITKLTPNVTRIGDIARAVVEAGSDMVSCINTLAA
                     MAVDVFSRRPKLANVVGGLSGPAVKPVALRCVYEVLQAVSCPVIGIGGIQNATDALEF
                     LLLGARAVQVGTANFVRPTVTVEILEGMRRFLQFQKIRNIEDYIGTLDTSCLFPFAEE
                     TCPVPGTDGREA"
     gene            complement(8065..8649)
                     /locus_tag="PLM79_12755"
     CDS             complement(8065..8649)
                     /locus_tag="PLM79_12755"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV87228.1"
                     /translation="MLVLGIYGSPRENGNTDRMLDAFLEGAASRKCDIERIRVRDLEI
                     RGCLECGACDATGECVQEDDMVQVYPLLEQASHIVVASPIFFYGVTGQLKLLIDRSQA
                     SYMKRELAGKAGTPPDPAAVRKGFLLSAGATRGKRLFECTVLCVKYFFDAVNAEYAGD
                     LCFRQLEEKGAVDRHPTALEECRSAGEAFVRSSV"
     gene            9144..9554
                     /locus_tag="PLM79_12760"
     CDS             9144..9554
                     /locus_tag="PLM79_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOV87229.1"
                     /translation="MADCIFCRIVAGQIPCAKLYEDEYVLGFLDINPINPGHSLVLPK
                     RHYAVLTEVPDRELEACIGAAKRVARAVSRSVGSAGLNLLQNNGRAAGQLIEHVHFHL
                     IPRDPGDGFLTSWPGKPYPSGQMEAVLEKIKAAL"
     gene            9633..10229
                     /locus_tag="PLM79_12765"
     CDS             9633..10229
                     /locus_tag="PLM79_12765"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87230.1"
                     /translation="MSTIRLLPAIALILAMTSLSWADYQTGAAAFTEGDYTTALRELE
                     PLAVQGDASAQFLLGVMYGIGEQVRQDYAESFRWFHEAALQGHAAAQLCLGMMYREGK
                     GTEQNLPAAVKWFGKAADQGNANAQFILGEMYRTGEGVRKDPVQAHVWFNLAAIRGRK
                     DAAEARDSLARKMTSRQIAQAHAKARNWKRSSQKNPAR"
     gene            complement(10231..11202)
                     /locus_tag="PLM79_12770"
     CDS             complement(10231..11202)
                     /locus_tag="PLM79_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HOV87231.1"
                     /translation="MEKGISRFLEHHFRHFNAAALMDAAKAYRDFVDSGGHMLVALAG
                     AMSTAEIGLTLAEMIRQDKVHAVCCTGANLEEDLFNLVAHDFYVRVPHYRDLSPAEEE
                     DLLRRGMNRVTDTCIPEEEAVRRIGDVMLQCWKDAENAGEQRLPHEYFYQVIRSRALE
                     ASYQIDPRDSWLVAACEKDLPLFVPGWEDSTVGNIYAAHCMRGNLGNLYTVRGGVEYM
                     LKLADWYTRVGKDRSMGFFQIGGGIAGDFSISVVPILRQELHLDVPLWGYFCQISDST
                     TSYGSYSGAVPNEKITWEKLGVDTPRFIVESDATIVAPLIFSYVLGA"
     gene            complement(11430..12650)
                     /locus_tag="PLM79_12775"
     CDS             complement(11430..12650)
                     /locus_tag="PLM79_12775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV87232.1"
                     /translation="MKNLWFYRTRSALCASAVALSVGVLFSVLSAVGGFEKALVRESR
                     SMGIDFVVAPANCPHEVASLLLYGSVSPSFLDESIVVEIRKTEGVSLATPLAILSVSG
                     IDGKEKLSVYGYEMAHLAKIKPGWQVLGEIPVGWDAIAEQKVLIGWDVAGRYGLEIGD
                     RLPVSGLAKSLVVRRMVRKTYGRDDAFVFAPLRVVRQMAVSVLGMQVIEVKDPSCALQ
                     SKITPDPVSAVAIQVEDPARVRQVIRDIETRLPGIEIVPVAQAMESLSELASSAGVFV
                     LAVSVIVLLVSMTAVMNSLLMTVFEMRGQLGMMRAMGASGSDILRFILWFSVIPATAG
                     GITGIGLTHLASPLIARLVETAAPRVWLVMEGFDPVIALKCLLLSTALGCGAGILPAW
                     RASRLKPLDAVRTG"
BASE COUNT         2573 a         3853 c         3859 g         2517 t
ORIGIN      
        1 gcctggatga aaaggcagtc ttcatcaggc tcttggattt caagaaaaac ctccgtattc
       61 cctccctgtc gaatgacccc ggcaagcacc tcgaaaaact cccccagctt tttcatggat
      121 cccggaggga cctcttccag gcttacgcgg agtctgcctc cgtccgggag ttccaggtcg
      181 gagaggtcga gtgatttcac cgtcgagcgg gcgcctttgg agtaaaggct acccaggcgg
      241 ggtaaaaggg gcaggattgc cctcagggct tcggtgccgg aaaggttgag cacattgcgc
      301 ttggcagtga cccagcctct cctggacgct tccgatggaa cgatgatgac atcgtcggat
      361 ttggcctgat gcacgatggc gggtggccca tggacgagcg tgtcgggctt ttcggtctgt
      421 cccggcttcc ccgtgtaggt atagagcttg tcggaacgaa tgagcttctc cgccgcctcc
      481 gtgatcttgt ccctggggac ttccccatgg cgctcacgca cttcgtccac cacgtcggaa
      541 atagtgcagg cctttttttc aaaggcaaga gaggtgaccc actgctctat cttggcggga
      601 tcgatggtct ccccgatcca ttcccgctgt ttggcaccct ggacggtgac gatggaccaa
      661 cccggggcct tgagatccag attgaggggg ataccccccg attccgtgct gtaaatgacc
      721 tcaggctttg tgctctctgc gcttcccatg cggaaaagac accatatgcc cctgttcacg
      781 ccttcccgga tgatggttcc caggagggtc gattgttcaa gaacaggcca gtgacgcttg
      841 cagagaaagc tctcctcgat tgcggcaagt gcgggcgtct gatccaactg gaaaaaaaga
      901 ccggagaggg cctggagggt ctcgagcgtg gcggcccggt ccgccgtgat gagctcgcct
      961 tccgcctgaa ggatccggcg catttcctca aggatggaaa agcccccttc tccccctccc
     1021 gtcttgattt ctttgtgtgt aatctggccg gaagcggaag gataccagac ggtgtcgtag
     1081 atctgcgcaa gggaggtctg aagggccaat tccctttcag cctggcgact gacgaattcg
     1141 ggatcattga gcttggaggc gtggattccg tagttctccg gcttttcctt gagcctccgc
     1201 atggcgagga cccagcgggc caagtccttc aggcgattga gggtttccat gtagcggcgt
     1261 accctgtcct cgttccatac ctcgcccttg acgttgaccg tgctgggcac cagcagaaag
     1321 agctggtttt gctgtatgcg gggccggttt gggcccactg tggtgaggaa gtccatccca
     1381 tcgaccttct tggcgttgac ggcaatgacg gcgatggatg gttggttcac attgtccggc
     1441 atgtgttccg gaagcgagac gtcatggatc acgcgaaacg agctgcccgc tcccgtgatg
     1501 agtttcctca ccgaggtgtc gagcagctgg tcgacggagg gttcacttat gcctctccgt
     1561 atttgtgcaa ggacgatatt gaccgagggt tcgaggctcg cgtaatagcg gccatggtcg
     1621 gagcgaagat aataggcgct ttccttgatc tcgcggaggg ccgtttccac ctggggtggt
     1681 gtcagacccg ggaaagcgat ctcgaacagg gcgtccttct ccgagatgcc aaagagattg
     1741 gagccaagac cctgacttcg gccgaccaga ctgtgaagaa ataccgtctt ccacgtgtat
     1801 tcgtggaggg ggtggccggc ggggtgcggg ttcttcctgt ctgcaagctc tgcctggctg
     1861 cgccccgtgt cgagggagga ggtgtccggc ccgccgatat cggcgttgat gaccggcagg
     1921 aggtcaccgc ttcccgtccg accgatgatc tcgttcaagg ttcgggaatc tctgaagtcc
     1981 acgtgacagc tgtggatcat gggaaccttg agcttcttgc cccatatgct ccggatagtc
     2041 agagccagaa ctcggaggac cccacgggtg ccctggaagg tttcgagagt ggagagcttg
     2101 gtgttgagga agtcgatgaa tgtgggatgg aagggatagt gggaggccat gacgtcccgg
     2161 aagtcgctcc gaagggccct ttcgggaaga gcggcaccgc tcttggcata catttccatg
     2221 taggcgttcg cggcatcgac ggcggctcgt ggatcgcagg aatcaaaaag ccgaacggcc
     2281 aggatccgtg agatttcgcc tgcgcgaacg ggaaccaccg ttgatgcatc ccgaaagacg
     2341 acgcttcggc ttgcagcctc ggccctctgc gccaatccca gggcttcgtc ctcggagacg
     2401 ttctcacccc tcacttgaga caggagatcc ctgagacgtt cggtctgacg accgaaggcg
     2461 tccgactggc ttgccagtgt catcacgacg gagatgcccc tgtgggtcct tgcgtagccg
     2521 ttgagcacca tgaggaacgc cgccagctgt tcccctccgt tggggcgggc cgcttccagg
     2581 cgtgcagcat actgcgccaa ctcgtcaagc ataatgatgg cttttcggcc ggagaggatg
     2641 cggtcgagaa agcctgcgtc cggagccgca aaggaggtcg cttcctgttc gacggttcga
     2701 tagagggctt ccccgccgat ctgataggca atttcgcccc agagggtgta ggggacgagc
     2761 ctgacgccct ccggcttgtg cacggggatc tcatcgccgg cgataccgac caccgacact
     2821 tcacctgcag ccggcagaag agagctgtcg acgatgtttt gcgtcactga cgcaagttcg
     2881 cgaccccgaa acccaagatg aacaagggcg atgagaaggt gggttttgcc ccctccaaaa
     2941 gctgtttcaa ggcgatggat tgccggaggg ttctgccccg cgatccgccc gaaaacatcg
     3001 gcgagcacca tgcggagacc ttcggtgggg tatgttccgt caccgaagaa ctggtccgca
     3061 tccgtgtaaa gcttgtgggc tatggatgtt ccgccacggt atgcgctcat aacctggctc
     3121 aggccggcac tgaagatttc ggggttgaat gtgccctcca gaatgtcctt tctgggccag
     3181 cagacctcga ggatggatcg cagcatatcc acttctcccg tgagctgaaa tctatgtgca
     3241 aggaacgtat tgggtcttta aaccatacac tgcacacttt tttagaccag gttggacctg
     3301 aatacaagcg aagcggcccc cgacgatgcc ggacaacttg cggaaagatg gcggttgacc
     3361 aaactcccgt caataccgga cagcagactt gttggacgaa tactacgttg cgtgcaagat
     3421 gacatgtatg gtacaatgca atcctggatg taaagggggg atgccgatgc caaggaaagt
     3481 caaggtgtcc actgaggtat ttgagtcggc gaaggaaacg attgggcggg ccaaaacggc
     3541 tgatgagctc agagcggctc aggctgtcgt tttgcccatc cttctcggca tttccgacca
     3601 cgctgtcgga caggtgattg ggcgttcccg tgtgagcgtg gtccgaatgc gcaaggagtt
     3661 taagcaactg cacaccccaa aaggggtcgc ggctcgcaac tggggaggcc gccgctccgg
     3721 ctacatgacc gtggaccagg agcgggaatt cctttcccgc ttcttcaatc acggatcgca
     3781 gggaagcgcc gtcaacgcgg acgagatcaa ggaggccttt gaagtgctgg tggggcacaa
     3841 ggtggcgaag acgaccatct atcgaatgct cgatcgtcac ggccggcgaa agatcacacc
     3901 aagaccgcat catcccaaga gtggggccaa ggccagaaag agttttacaa aaactgcgaa
     3961 aaatggtgac tcgatgcggt gaagcgccga caagcacagg agatcgcctc cgcttgaggt
     4021 tcctggaaga ggggcgcttt ggaaggatca gcacaccccg gcgctgccgg gctcctcgcg
     4081 gcgtgcgccc aagggtgcgc gaggacaggt atgcctacgt ggccgtcact cctcataacg
     4141 ggatgatgga tccccatatt ctgatcgagg tgagcgagca ggcagtgtcc cttttttttt
     4201 tacggaggtg tgtagccgcc acccggatga attcatcctc atggggctcg acggagccgg
     4261 ttggcatagg gcagggaatc tgagggtgcc ggcaaacatg cacctgatgt tccttccgcc
     4321 ttatggtccg aagctgaacc cggttgagcc tatctggaag agcatccgcg aaaaccggtt
     4381 cccaaaggcg gtcttcaaga acgtggacgg agtggaaaac cagccggaga aggctcttgc
     4441 cgcccttgaa agcggtccgg acgccgtagc tcggctgacc gggttcccat ggatccgttg
     4501 gtatcagcat gcacgcacct tagtattgaa tgcacgttga aagcgaccac gtcgaacaga
     4561 ttccggccgt atgttgggga tagcgttggg ggtaagaaag caaaggcccg gacgctttcg
     4621 gctaagccct tgattttctt ggagggcgac tcggggatcg aacccgagac ctttggttcc
     4681 ggagaccaac gctctatcca ctgagctagt cgccctcacc gggaaacacc ttccaccgtc
     4741 tttcgaccat gtggctgagc ttgggtaaca caggcgggaa cgaatgtcaa tccgcaggat
     4801 tcagggcggc cccggcaatc acggccgggc gcaggaagga gtcatctcct ccttggttgc
     4861 ttcccggatg tccttgataa gaatgtcgaa ctcggcgtgg gttccggccc ggggatcgtt
     4921 gaagtcggcc acgatcacgt cctccttcaa ttccttcacc aggaactgaa ccaggcactt
     4981 ttttttcatc tcccggtaca tcttgccggg tttgagccgc tcctgcttgt agtcggctcg
     5041 aggcagttcc cggattaagg cctcgtccgc atggccgaag agttcctcgg gaggaacgta
     5101 tacttggact cgatctccca cgcacttgtt catgatggcc gtttcaaccg aaggatattg
     5161 gacatccacc ccaaagacga aactgcagac ttcgggaggg acctccgtga cctccccgga
     5221 cggggacacg gtcttgatgg cgaactcgat ggtgactttc atgcactctt tgactcgcag
     5281 catgggaatc ctctcttgca gggggatttt ggataccgac ccaacataat gcccggtggg
     5341 ttattgtcaa taaaaccttg ctctcccttt cgattgaggg taagcaatac gcgacgtgaa
     5401 gacacagtgc cacggaccat ggaaggcgat gttcccatga caacccggcc ggaaccctgc
     5461 aacccgccgt ccccttccct cgaacgccat gtgtttcccg gtctatcccg ggtcccgggg
     5521 ttggtgcacg ggattttcaa gcggcatgga ggcgtcagtc ttccgcccta tgccgccctc
     5581 aacgtggcat gggacaacgg ggacaacccc cgatttgttc atgaaaacct ctcgaggatc
     5641 gagaaggacc tggggctgga ctgcctggcg gccgggcggc aagtacacgg ggaccgaatc
     5701 ctcctggtgg acgaaaacgc cctgggccgc gccgagaccc gcggccacct ggtcatcacg
     5761 ccgccggggg atgccctggt cacgcgactg cgcggggtgg ggctcctcat caagatcgcc
     5821 gactgccagg ccgtttttct cgtggatccc gtccaaaacg tcgttgccaa tgttcattgc
     5881 ggatggcggg ggagcgttca ggagcttccc gccaaggtgg tacgtttcct ccgggaccgt
     5941 ttggggtgcc gccccgagga ccttcttgcg gccgtcagcc catccctggg tccgtgctgc
     6001 gcggaattcc gcaactaccg caccgagctg cccgcctcct ttgcggccta tcaagcggcg
     6061 ccgctgcact tcgacttctg ggccgtcacc cggaggcagt tggaggaggc cggggtccgg
     6121 ccggaccgta ttgaaattgc ccaagtctgc accgtgtgcg agtcccggga ttacttctcc
     6181 taccgggggg aaaaggtgac gggacgaaac gccgcggtca tcggctggca agcctagaag
     6241 caccgtgtcg aggaacctca gatgacttcg ttccaggaag acggtcgggt ggtccttcac
     6301 gagaaggtgt cccagcagac gtaccggctt cgtattcatt ccccccggat ctccgcacag
     6361 gcactgccgg gccaatttgt catgcttcaa gtgcaaaagg ggatcgatcc cctgctgaga
     6421 agaccctttt cctttcatcg cctccttccc ggagaagact ctttcgaaat cctttaccgg
     6481 gtggtgggac gagggacctg ggtcctttcc cggacgcctc cgggagccgt cctttccatc
     6541 ctgggcccgc tgggaaacgg gttcctaatc ccctctcctc aaccgtccgg ggtgctgatc
     6601 attgcggggg gaatcggcat tgcgcccctc tatgagctgc tgatccggct gtcggacggg
     6661 cgaattccgg gccgggagcc gcccctgcgg cttctctacg gagcgcgcac cgcagacgag
     6721 cttctcccca tgagctgctt ccacgagttg ggggcttccg tttccttcgc cacggacgac
     6781 ggcagtctcg gataccgcgg gaccgtcccg gagcttcttg ggctgctgac ggccaaagaa
     6841 aagctcgaac ctcagctggt ttacgcctgc gggcccttgg ggatgcaata ccgggtggcc
     6901 gtgtgggtga aggaacgggg aatcccctcg cagctttccc tggaatccct catggcctgc
     6961 gggttgggag cgtgtctcgg gtgcgcgctg cccgctcccc atcccgacga cccgcttcga
     7021 ccccattatg tccacgtgtg caaggacgga cccatctttg aggcaggagc cattgaatgg
     7081 caaaagatcg tggggcaaca aacgattgcc ccgacctttc tgttcagcta gggcccctga
     7141 gacttcgaaa ccccgtcatg gtcgcttcgn nnnnnnnnnc cgggagttgg gggccgtggt
     7201 ggtcaagggg atctccctgg aacccctccc ggggaatccg cctccccgcc tggtggagac
     7261 ccccgggggc ctcatcaatg ccatcggcct cgagaacgtg ggtctcgagg cgtttctggc
     7321 ccaaaagctt ccctacctcg agggcatgag cgttccggtc atcgtaaaca ttttcggaaa
     7381 caccctcgag gaatacgggg aacttgcccg aaggttggac ggggtgccgg gaattgtcgg
     7441 cctcgagatc aatatcagct gccccaatgt gaaacaagga ggaatggttt tcgggaccga
     7501 cccctccatg gcggcctcgg tggtgggaac cgtccgaaag gcggcatctc tgcccctcat
     7561 taccaagctg acccccaacg tcacccgcat cggagacatc gcccgggccg tggtggaagc
     7621 cggaagcgac atggtctcct gcatcaacac cctggcggcc atggcggtgg atgtcttttc
     7681 ccggaggccc aagttggcca acgtggtggg gggattgtcg gggcctgcgg tcaaacccgt
     7741 ggcgctacgc tgcgtctacg aagtcctgca ggcggtttca tgccccgtga tcggcatcgg
     7801 cgggattcaa aacgccaccg atgccctgga gtttctcctc ctgggcgccc gggccgtcca
     7861 ggtggggacg gccaacttcg tgcgccccac ggttaccgtc gagatcctcg agggaatgcg
     7921 gcggttcctc caatttcaga agatccggaa catcgaggac tacatcggga ccctggatac
     7981 ctcgtgcctg ttcccttttg ccgaagagac ctgcccggtg ccggggaccg acgggcgcga
     8041 agcgtagcgg gcgaggcgcc ggtcttagac cgaggaccgg acgaaggctt cgcccgccga
     8101 gcggcattct tccaaggccg tgggatgccg atccacggcc cccttctctt ccaactgccg
     8161 gaagcagagg tcgcccgcgt attcggcgtt gacggcatcg aaaaaatact tcacgcaaag
     8221 aacggtgcat tcaaaaagcc tcttgccccg ggtggctccg gcgctcagga gaaacccttt
     8281 ccggacggcc gcgggatcgg ggggggtccc tgcctttccg gccaattccc gcttcatgta
     8341 ggaagcctgg gaacggtcga tgaggagctt gagctgaccc gtgacgccgt agaagaagat
     8401 cggggaagcc accacaatgt gggaagcctg ttccagcagg ggatagacct gcaccatgtc
     8461 gtcttcctgg acacactccc ccgtggcatc acaagcaccg cattccaggc agccccgaat
     8521 ctcgaggtcc ctgacgcgga tgcgttcgat gtcgcatttc ctgctcgcag ccccctcgag
     8581 aaaagcatcc aacatcctgt cggtatttcc gttttctcgt ggggaaccat agatgccgag
     8641 cacaagcatg gaccggtctc ctttccgaaa tgagcggttg gccctgggca gccgtgcgat
     8701 tcaaccgaga tatccctggg cggcttccgg tgaaacagcc cccgagaagt cctgggccgg
     8761 ggttcccggc ctgccgaaaa catggggccg tcggcaaaca ctcgaggacc cctcgattcc
     8821 tccgggtcct tttcgagcgg caacctccgg aaaccggcaa atcccgggcg cggtgcctgc
     8881 caccggaaac ggcccttcca taagcggtac ccattataga ccaggggagc ggccaaggac
     8941 acggctgaaa cacttctttg acaggacccg ggccccctcc ggcaaatttg cccgggggaa
     9001 gcctttcccg ccccggccct cctcccgggt ccggattcgc ggctgcaggg gacatcattc
     9061 tcacactgga cgaatcgatc gaaataccct aacttaaccc tcagcgcccg attcaccccg
     9121 gaggaaccat aaggaggaac cccatggccg actgcatttt ctgtaggatc gttgcggggc
     9181 agatcccctg cgcaaagctt tacgaagacg agtatgtgct cggcttcctg gatatcaacc
     9241 ccatcaaccc gggtcactcc ctggtgctgc ccaagcgcca ttacgcggtg ctcaccgaag
     9301 tcccggacag agaactggaa gcctgcattg gggcggccaa gcgggtcgcc cgggcggttt
     9361 cccggtccgt gggaagcgcc ggtctgaacc ttctgcagaa caacggcagg gcggcgggac
     9421 aactgatcga gcacgtccac tttcacctga ttccccggga cccgggggac ggatttctta
     9481 cctcctggcc cggcaaacct tatccgtccg ggcagatgga agccgtgctc gaaaagatca
     9541 aggccgccct ctagagacgg ctccccggtg tttgccccgg gagtcacccc aacggcctgt
     9601 gctgacaaga accatccatt ggagcccata gagtgagcac gattcgactt ttgccggcta
     9661 ttgcactgat cctggccatg accagcctgt cgtgggccga ctaccagacg ggagcggcgg
     9721 cctttaccga aggggattac accaccgccc ttcgggaact ggaacccctg gcggtacagg
     9781 gcgacgcatc cgcccaattt ctgttggggg tgatgtacgg aatcggggaa caggttcgcc
     9841 aggattatgc cgaatccttc cggtggttcc acgaggcggc cttgcagggg catgccgccg
     9901 cccagctttg cctgggcatg atgtatcgcg aaggcaaggg caccgaacaa aacctgcccg
     9961 ccgcggtaaa atggttcggc aaagccgccg accagggaaa cgccaatgcc cagttcatac
    10021 tgggggaaat gtatcggacg ggcgaggggg tgcgaaaaga cccggtgcag gctcacgtat
    10081 ggttcaacct ggccgccatc cggggccgaa aagatgccgc cgaggcgaga gactccctgg
    10141 cccggaagat gacctcacga cagatcgccc aggcccacgc caaggctcgc aactggaagc
    10201 ggtcgtccca aaagaacccg gctcgctagg tcaggctccc aggacatagg agaaaatgag
    10261 cggggccaca atggtcgcat ccgattcgac aatgaaccgg ggagtatcca ctcccagctt
    10321 ttcccaggtg atcttctcgt tgggcacggc gccggaataa gagccgtagc tcgtggtgga
    10381 atcgctgatc tggcagaaat agccccatag gggaacatcc aggtgcagtt cctgtcgcag
    10441 aatgggcacc accgagatgg agaagtcccc ggcaatccct cctccgattt gaaagaaacc
    10501 catggaacgg tccttgccca cccgggtgta ccaatccgca agtttgagca tgtattccac
    10561 gccgccccgc accgtgtaaa ggttcccaag gtttccacgc atgcagtggg ccgcgtagat
    10621 gtttcccacc gtcgagtctt cccatccggg gacaaagagg ggaaggtcct tctcgcaggc
    10681 cgccacgagc cagctgtccc ggggatcgat ttgataggaa gcctccaatg cccggcttcg
    10741 aatcacctgg tagaaatatt cgtggggcag gcgttgttcg ccggcgttct ccgcgtcctt
    10801 ccagcactgc agcatcacat ccccgatgcg gcgaaccgcc tcttcttcgg ggatgcaggt
    10861 atcggtcacc cggttcatcc ccctgcggag cagatcttct tcctcggcag gactgaggtc
    10921 ccggtaatgg ggaactcgaa cgtagaaatc gtgagcaacc aggttgaaga gatcttcctc
    10981 gagattggcc cccgtgcaac agacggcatg aaccttgtcc tgccgaatca tttcggccag
    11041 ggtcaggccg atttccgcgg tgctcatggc cccggccagg gctaccagca tgtgcccccc
    11101 cgagtccacg aaatcccggt aggccttggc cgcatccatc agggccgccg cattgaagtg
    11161 ccgaaaatga tgttccaaga aacgcgagat acccttttcc atgatcgatg aaccctctct
    11221 gctgaaagct gtccccgccg gggaaccttt cccgggaatg aagaaaccat actgttttcc
    11281 gatagttatg gaatcatggc ccggggtttg acacggccaa aggtacccta tttttgcccg
    11341 tggttcaagt cataccaagg aaattcagga agtcgacggg ccgaaacccc ttccaaacac
    11401 cgggaaagaa gggcggacgg aagagtctat cacccggttc gaacggcatc gaggggcttc
    11461 agccgggagg ctctccatgc cgggaggatt ccggcaccgc acccgagggc cgtggacagg
    11521 agcagacact tgagggcgat cacgggatca aagccttcca tgaccagcca tactcggggc
    11581 gccgccgttt ccacgaggcg tgcgatcaag ggggaagcca ggtgagtgag gccgatgccc
    11641 gtgatccctc cggcagttgc cgggatcacg gaaaaccaca ggatgaaccg aagaatatcg
    11701 gacccggatg ctcccatggc ccgcatcatc cccagttggc ccctcatttc gaaaacggtc
    11761 atgagcaatg agttcatcac cgccgtcatg ctgaccaaaa ggacgatgac ggaaaccgcc
    11821 aggacaaaga cgccagccga ggaggccaac tccgagaggc tctccatggc ctgggccacg
    11881 gggacgattt ctatgccggg aagcctggtt tcgatgtccc ggattacctg cctcacgcgc
    11941 gccgggtcct ccacctggat ggcaacggcg ctcacggggt ccggagtgat cttggactgc
    12001 aaggcgcaag agggatcttt gacctcgatg acctgcatgc ccagaaccga caccgccatt
    12061 tgccggacca ccctcagggg agcaaagacg aaggcgtcgt ctctgccgta tgtcttccgg
    12121 accatgcgcc tcaccaccag gcttttcgcc aggccggaaa cgggaagccg gtccccgatc
    12181 tccaggccgt atcggccggc cacgtcccag ccgatgagga ccttttgctc ggcaatggca
    12241 tcccagccca cggggatctc tcccagcacc tgccagccgg gcttgatctt ggccaggtgg
    12301 gccatctcgt atccgtagac ggacagcttc tcctttccat cgatgcccga aacgctcagg
    12361 atcgccaggg gagtggccag gcttaccccc tccgtttttc gaatctcaac cacaatggat
    12421 tcatcgagga agctcggcga gacactcccg tatagaagca gggaggccac ctcgtgggga
    12481 caattggcgg gagcgaccac gaaatcgatt cccatggatc ggctttcgcg gaccaaagcc
    12541 ttctcaaaac cgcccacggc ggacaagacc gaaaacaaca cccccacgga aagggccacg
    12601 gcactcgcgc acaaggcact ccgagtccga taaaaccaca aattcttcaa ggccaacccc
    12661 caaacagtca tccctgtccc ccagtcgtat gggaatccgc ccgtctgctc ttggcggtcg
    12721 accgaccctt aggtgggtgt aggtgcagtt ggtggggagc tccgggttgc ttgccatcat
    12781 gacccgacgt ttgagactca gatccttttt gt
//
LOCUS       DAOOVC010000107        12765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3240_length_12765_cov_7.053816, whole genome shotgun sequence.
ACCESSION   DAOOVC010000107 DAOOVC010000000
VERSION     DAOOVC010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3240_length_12765_cov_7.053816"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1344
                     /locus_tag="PLM79_12780"
     CDS             163..1344
                     /locus_tag="PLM79_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HOV87233.1"
                     /translation="MSTVLIIGAGGVGSVVTHKCAQVPEVFSKIVLASRTLEKCQRIR
                     DRLSRPIEIAQVDADDVRQLTRLMENTRPDLVVNVALPYQDLTIMEACLETGVHYLDT
                     ANYEPPEEARFCYRWQWDYHDRFRERGLMALLGCGFDPGVTNVFCAHALKNHFDEIHF
                     VDIMDCNAGQHGHPFATNFNPEINIREVTAPGKYFEDGRWIETEPLSVCETFDFPGIG
                     PREMYLMYHEELESLAKNLPGLRRIRFWMTFSREYLTHLRVLENVGMTRIDPVDFQGR
                     KIVPLQFLKALLPDPASLAPSYSGKTNIGCMIRGLKDGKPRRYYVYNICDHGDAYREV
                     KAQAISYTTGVPAMIGALMILTGTWRGAGVFNVEQFDPTPFMEKLAEYGLPWTEEFLD
                     P"
     gene            1356..2492
                     /gene="nspC"
                     /locus_tag="PLM79_12785"
     CDS             1356..2492
                     /gene="nspC"
                     /locus_tag="PLM79_12785"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOV87234.1"
                     /translation="MIRELAPLPTPCYVVDEASILRNLRVLDSVRERTGCRILLALKG
                     FALFPLFPLIRGYLNGVAASSVHEARLGFEEFGGEVHTFSPGFKEEDMGELLRYSSHL
                     VFNSFSQWDFFRPILRSFPQAPQVGLRVNPEHSEVKVPLYDPCAPFSRLGVTRESFRG
                     RNLDGISGLHFHTLCELNADSLERTLAAFEEKFGDCLPGLKWVNFGGGHHITRQDYDL
                     DRLCRILTDFRKRYPLEVYLEPGEAVALHAGWLVASVVDLVRNRMDIAVLDTSAAAHM
                     PDVLEMPYRPEILGAGRPGEFPHTYRLGGVTCLAGDVIGDYSFPEPLEVGTRLVFLDM
                     AHYTMVKNTTFNGVQLPAIAVRDLENRVRVVRSFGYEDYRNRLG"
     gene            complement(2489..3445)
                     /gene="miaA"
                     /locus_tag="PLM79_12790"
     CDS             complement(2489..3445)
                     /gene="miaA"
                     /locus_tag="PLM79_12790"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOV87235.1"
                     /translation="MEAYAPPPPVVLLAGPTAVGKTTVSLDLAVALGCEIVNADSMQV
                     YRFMDIGTAKPGREERARVPHHLLDVADPDEPFDAARYLRMGQGILSELHGRGVLPLV
                     VGGTGLYMKALTRGLCEGPPSDEGVRAILRREEKEKGLEFLFEELSRVDPAAARRLHP
                     HDRQRILRALEVFRLTGQPLSARQSRHGFSQSPYRTVKVCLHRDRQELYERIDRRVER
                     MMEAGFVEEVRRLLDMGYGPELKPMQSLGYRQIVSFLRGACSLDSAISRIQRDTRSYA
                     KRQLTWFRGDPEFHWFHPRDRQVILEFVRAELRRVPDTAGQR"
     gene            complement(3454..5262)
                     /gene="mutL"
                     /locus_tag="PLM79_12795"
     CDS             complement(3454..5262)
                     /gene="mutL"
                     /locus_tag="PLM79_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOV87236.1"
                     /translation="MGKIVILPDVLCNQIAAGEVVERPSAVVKELVENSIDAGSRRIA
                     VYALQGGRKEIRVVDTGAGMSSEDALLALERHATSKIRSTEDLQAIRTLGFRGEALPS
                     IAAVSRFELVTRDGDSLAGTKIRMEGGILRDVRETGCPVGTMITVRDLFFNLPARRKF
                     LRSTDTEMSHISDHFLRLALAHPAIHFQLFHEERTQYDFAPSPGLEDRAAQVLGVESA
                     RKLRSFSGSNGSLSVRGLAGLPELQRTNAHSLFLFVNGRPVWERMLSRAVLNAYDTLL
                     PAGRFPVAVLFVDLSPALVDVNVHPTKREVRFRNPAEVLETVRSVVREALVNRSGAGQ
                     VGGTTSWPISPESLWDRKRQYSMETQTRFHRPLRGLSGELAEAISPVDPEGAAEPLES
                     VAARGGEEKFEGEQSAAGPPVFSSLPILGQLANTYILLEARDGLLLIDQHAAHERILY
                     EELSAQTQPDAGQRLTQPVVLHLLPREAAVLRRWLPQLAAVGFGVEPFGQDSFLIDAV
                     PSVLGLCRPDELLRELLDMSREEEKTARWDFLAALAKTAACHGSVRAGQRLAVQEIRH
                     LLETLDRRKIPLTCPHGRPIWHKLTYPEIARFFRRT"
     gene            5406..6854
                     /locus_tag="PLM79_12800"
     CDS             5406..6854
                     /locus_tag="PLM79_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87237.1"
                     /translation="MQSNPYQSLVSLLSNILEAYTTAFFLLDPKGRQLKLAASQSLSK
                     FLPPRVSLPLEQSGILSQVHKVGQTIHLDKVHEAASNLAATVPFYREGESHIKGLFAI
                     PVGDGAGVLYVDTKYSWGFNDKQQKWIHEIAAVLGELVSRQNCLAQQKTYGRILELLH
                     SLDQATLKQSALEDYCNEVIRELADFLAAEYGFVAVRDPGEAHHYLFACTPNVPRSLT
                     TQRFLTKQGLVGWVFQNQRPLLVTRLNPQSPDHFLFTPSEALPHHGTFWGMHLALSLG
                     HAVVPAFMSRTPTEWSVDDQYAVSHVLLFLNLLLERFYFRDECEHLQCYEFSTGLYNA
                     PTFESRMEAILNASMRDSTPFTLSLVQFEPWQLLWTKASPKEVRQYQMELASTVCEAL
                     PSNTLVGHLAENRFGLLFLGASPEEARHLQAHLLDFDPRAFARKTKGVRLKPYLSMAA
                     FPQDGTRVEELWPTAYQRLYAAFRTKSDSAGQ"
     gene            6942..7964
                     /locus_tag="PLM79_12805"
     CDS             6942..7964
                     /locus_tag="PLM79_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOV87238.1"
                     /translation="MLDRLLSYFSKDLAMDLGTANTLIYLRGKGIVLNEPSVVAINQD
                     SHQVIAVGQEARNIIGRHGQRVVTIRPLKDGVIADFEVTSVMIKYFLAKILKRRQLVR
                     PKLVVAVPTGITAVEKRAVIEAAEQAGARHIHLVEEPMAAAIGAGLPIDQPFGNMVVD
                     IGGGTTEVAVISMFAVAYSESVRVAGDEANEAILRYMQRERQMIISEVLAENVKMKIG
                     TAAPLEEPQSMEITGKEILTGIPRTFTITDEEIREAIREPVTTIVESVKRALEKTPPD
                     LASDIHEKGFWLAGGGALLKGLDRLLAQSTNLKVNIADDPLTAIVRGAGAVIEHLDYY
                     RQVFIN"
     gene            complement(9067..9852)
                     /locus_tag="PLM79_12810"
     CDS             complement(9067..9852)
                     /locus_tag="PLM79_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87239.1"
                     /translation="MKRWINICLGSFVWFPVLVLLWGNAFPVWAEGRSETQKALDEYE
                     AAVDARRQMLIDEAGREKYGEEKWEEMKRNAKKKGIPWYVLYDTNANEYQQPFTRPEK
                     ILTANNLASAVSGNPSKCSDAKWQVGVLANYLRNNPGDSEAADAMSKLQAAIAANCPK
                     EPPPPPEDSSPPPPPSISFGEVTGQAFDPETGVLTYTDGEGVHTVNVPPGVTVNLVDP
                     EGNTTKVTRPPKPPKPPTDDSTGGGKGTGSGPSGGGSGGGGGG"
     gene            complement(10640..>12765)
                     /locus_tag="PLM79_12815"
     CDS             complement(10640..>12765)
                     /locus_tag="PLM79_12815"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HOV87240.1"
                     /translation="NVPPTPANYAQRSGRAGRQGQPGLIFTYCGAINSHDQYFFHQRT
                     EMVAGSVRPPRLDLTNEALVKAHLHALWLAQVRLPLGRSIEEVIDTIDESLPMREHAA
                     ATIQLSESSAYALHERARRVLAVDLGGLDGSDWFGDEWITHVLNQAPVEFDRAFNRWR
                     ELYRAASRQLVESQNLVRRAQRREEQDDARRREEEALRQLNLLRQINTTREESDFYPY
                     RYLASEGFLPGYNFPSLPVRAWVPREEGEFIARPRFLALREFGPGNIIYHEGRKWELM
                     SFQAPPGGLDERRTQKRFCNVCGTFCDMGQGFELCPNCRTTFDGSNSLIATLLEMPNV
                     RCRGRDRITCDEEERRRRGFDLTVAYRFAQESAGNPRIQEADVVCGGTPVLRLTYAPA
                     ATLMRVNHGWRSATQPGFLVDFENGEVLSGGTATGAQQGRPRRPELVRLCVQGTQNLL
                     LVRFSRPELRNDPVLESTLQYALQRGCEQFFQLEEAELAAERIGTGDHRAILLYEAGE
                     GGAGVLRRLVDEADGMARVAAEALTRCHFDVEGNNLRPDCHAACYECLMSFSNQHEAI
                     NLDRHGILVHLLDLVSSITRPRVNGRDWASHLAWLRSLTDSRSVLERNFLDILASRGY
                     RLPDYAQHAIPDPHCIPDFFYSPNVCIFCDGSVHDEPPQAERDRVLRSELRSRGFRVI
                     AIRYDRSIDEQIAQSPDVFGHGSSR"
BASE COUNT         2412 a         4078 c         3804 g         2471 t
ORIGIN      
        1 acatcctcgc ctaggccggc cgattgactc cccaaaaaca tgtttctata ataccccgat
       61 ttcgataggg ccctagttgt tccaccctcc tgtggaagcc cggtttttgc cccatggaga
      121 tgcaggattc caacgacttg agacgaaagg ggttcccttc atatgagcac ggtcttgatc
      181 atcggagcgg ggggggttgg cagcgtcgtg acccacaaat gtgcccaagt tcccgaagtc
      241 ttttccaaaa tcgtcctggc cagccgcacg ctggagaaat gccaaagaat ccgggaccgg
      301 ttgtcccggc ccatcgaaat cgcacaggtg gacgccgacg atgtccggca gctgacccgg
      361 ctcatggaaa acacccgccc ggacctggtc gtcaacgtcg ccctgccgta ccaggacctg
      421 accatcatgg aagcctgcct cgagacgggg gtccattacc tggacacggc caactacgaa
      481 ccccccgagg aggcccgctt ctgctaccgg tggcagtggg actaccacga ccggttccgg
      541 gaacgggggc tcatggccct cctgggctgc gggttcgacc ccggggtgac caatgtcttc
      601 tgcgcccacg ccctcaagaa tcactttgac gagattcatt tcgtggacat catggactgc
      661 aacgccgggc agcacgggca ccccttcgcc accaatttca acccggagat caacattcga
      721 gaggtgaccg ctcccggaaa atacttcgaa gacggccggt ggatcgagac cgagcccctg
      781 tccgtctgcg agacgttcga tttccccggc atcggaccca gggagatgta cctcatgtac
      841 cacgaggagc tggaatccct ggccaagaac cttcccggac tccgaagaat ccgcttctgg
      901 atgacctttt cccgggagta cctgacccac ctgagggtcc tcgagaacgt gggcatgact
      961 cgaatcgatc ccgtggactt ccagggccgc aaaatcgttc ccctccagtt tctcaaggcc
     1021 ctcttgcccg acccggcctc cctggctccc agctactccg gcaagaccaa catcggctgc
     1081 atgatccggg gcctgaagga cggaaaacct cgaagatact atgtctacaa catttgcgac
     1141 cacggggacg cctaccggga agtgaaggcc caggccatct cctataccac gggagtcccg
     1201 gccatgatcg gggcgttgat gattctcacg gggacgtgga ggggcgcggg agttttcaac
     1261 gtggaacagt tcgatccgac gcccttcatg gaaaaactgg cagaatacgg gctcccctgg
     1321 acggaagagt tcctggaccc gtgacggaac ccggaatgat ccgagaatta gcccctcttc
     1381 caaccccctg ttatgtggtc gacgaggctt ccatccttcg gaacctccgg gtgctggatt
     1441 ccgtccggga acgcacggga tgcaggattc tcctggccct caagggcttt gccctgttcc
     1501 ccctgttccc tctcatccga ggctatctca acggggtggc ggcaagctcc gtccatgaag
     1561 cgcgcctggg attcgaggaa ttcggcgggg aggttcacac cttttccccc ggcttcaagg
     1621 aggaagacat gggggagctc ctccgataca gctcccacct ggtgttcaat tccttttccc
     1681 agtgggactt ctttcggccc attcttcggt ccttccccca agccccccag gtgggcctcc
     1741 gggtaaaccc cgagcactcg gaagtgaagg tgcccctcta cgacccctgc gcaccgtttt
     1801 cccggctggg cgtcacccgg gagagttttc ggggccgcaa cctggacggc atttcgggcc
     1861 ttcattttca taccctctgc gagctcaacg ccgattccct ggaacggacc ctggctgcgt
     1921 ttgaagaaaa gttcggggac tgccttccgg gcctaaaatg ggtgaatttt gggggaggtc
     1981 accacattac ccggcaggac tacgatctgg atcgcctttg ccggatcctg acggatttca
     2041 ggaagcgtta ccccctcgag gtctacctcg agccgggaga ggccgtggcc cttcatgccg
     2101 ggtggctggt ggcctcggtg gtggaccttg ttcgcaaccg gatggacatc gccgtcctgg
     2161 atacctctgc ggcggcccac atgccggacg ttttggaaat gccctaccgg ccggagatcc
     2221 tgggggccgg gcggcccggg gagtttcccc acacctatcg cctgggggga gtgacctgtc
     2281 ttgcgggaga cgtcatcggg gactattcct tcccggaacc cctcgaggtg ggcacacggc
     2341 tggttttcct ggacatggcc cattacacca tggtgaagaa cacgaccttc aacggggttc
     2401 aactgcccgc cattgccgtt cgcgacctgg aaaatcgggt gcgggtcgtt cgaagcttcg
     2461 gctacgaaga ctaccgaaat cggctgggct agcgctgtcc ggcggtgtcc ggcaccctgc
     2521 gaagctctgc tcggacaaac tcgaggatta cctgccggtc ccggggatga aaccagtgga
     2581 actccgggtc cccgcgaaac caggtgagct gtcttttggc gtagcttcga gtatcccgct
     2641 ggatccggga gattgccgaa tccagggagc acgctccccg aagaaacgag acgatctgcc
     2701 ggtatcccag ggactgcatg ggcttgagtt cgggaccgta gcccatgtcc agcaggcgtc
     2761 ggacctcctc cacgaacccc gcttccatca tccgttccac ccgccggtcg atcctctcat
     2821 agagctcctg ccgatcccga tggaggcaca ccttgacggt ccggtacggg ctttgggaga
     2881 atccatggcg gctctgccgg gccgacaggg gctgtcccgt caggcgaaac acctccaggg
     2941 cacgaagaat cctctgccgg tcgtgaggat gaagccgccg ggcagcggcg gggtccaccc
     3001 gggaaagctc ctcgaacagg aattcgagac ccttttcctt ttcctctctt cgaaggatcg
     3061 cccgaacccc ttcgtccgaa gggggtccct cgcacaggcc ccgggtcaga gccttcatgt
     3121 agagacccgt gcctcccacc accaggggga gaacgccgcg cccgtggagt tcgctcagga
     3181 tcccttggcc catgcgaagg tagcgggcgg cgtcgaaggg ttcgtccgga tcggccacgt
     3241 ccaggaggtg gtgtggaacc cgggcgcgtt cctccctgcc gggcttggcc gtcccgatgt
     3301 ccatgaagcg atacacctgc atggaatcgg cattgacgat ttcgcaaccc agggcaacgg
     3361 ccaggtcgag ggagacggtc gtttttccta cggcggtggg gcctgccagg aggacaacgg
     3421 gtggaggcgg tgcgtaagct tccatgggtc cctctaggtt cgccgaaaaa accgggcgat
     3481 ttcgggatag gtcagcttgt gccagatggg gcggccgtgg ggacaggtga gagggatctt
     3541 tctgcgatcc agggtttcca ggaggtggcg tatctcttga acagccaatc gttgcccggc
     3601 gcgaacggac ccgtggcagg cggccgtctt cgccagggcg gcgaggaaat cccagcgggc
     3661 cgttttttcc tcctcccggc tcatgtccag aagctccctc agaagctcgt cgggacggca
     3721 caggcccagg acggagggaa ccgcatcgat gagaaagctg tcctggccga agggctcgac
     3781 cccgaagccc accgccgcca gttgaggaag ccaccgccga aggaccgccg cttcccgggg
     3841 aagcaggtgg agcaccacgg gttgggtaag ccgctgcccc gcgtcgggct gagtctgtgc
     3901 cgaaagttct tcgtagagga tccgctcgtg ggcggcgtgc tgatcgatga gaagcaggcc
     3961 gtcgcgggct tccagcagga tgtaggtgtt ggcaagctgc cccaggatgg gcagggaaga
     4021 gaagaccggg gggccggcgg ccgactgctc cccctcgaac ttttcttccc ccccgcgggc
     4081 tgcgacggac tcgaggggct ccgcagcccc ttcggggtcc accggagaaa tcgcctcggc
     4141 cagttctccg gataaacccc gcaggggccg atggaaccgg gtttgggtct ccatggaata
     4201 ctgccgtttc cggtcccaaa gggactcggg ggagatcggc cacgaggtcg tcccgcccac
     4261 ctgccccgct ccggaccggt tcaccaaggc ttcacggacg acgcttcgca ccgtttccag
     4321 gacctccgcc gggtttcgaa atcggacctc tctcttggtg ggatgaacgt ttacgtccac
     4381 cagggcgggg gacaagtcca caaagagcac cgcaacggga aatcttcctg cgggcagcag
     4441 ggtgtcatag gcattcagaa cggcgcggct caacatccgt tcccaaaccg gtcttccgtt
     4501 gacgaacagg aacagggaat gggcgttggt gcgctggagc tccggcagcc cggccaggcc
     4561 ccgtacggaa agggagccgt tggacccgga aaaggaccga agctttcggg cggattccac
     4621 gcccaggacc tgggcggccc gatcttcgag gccgggggag ggggcaaagt cgtactgggt
     4681 cctctcctcg tgaaacagct gaaaatggat ggccggatgg gccagggcca gccgcagaaa
     4741 gtggtcggaa atgtggctca tttccgtgtc cgtggaacgt aggaattttc tgcgggcggg
     4801 aagattgaaa aagaggtccc gcacggtaat catggtccct acgggacagc ccgtctcccg
     4861 aacgtcccgg aggattcctc cctccatgcg gatcttggtc ccggccaggg aatccccgtc
     4921 ccgggtgacc agctcgaatc ggctcaccgc cgcaatgctg ggcagggcct ccccccgaaa
     4981 ccccagggtt cgaatggcct gcaggtcctc cgtcgagcgg atcttgctgg tggcatggcg
     5041 ttcgagggcc agcagggcgt cttccgagga catcccggct cccgtgtcca ccacccgaat
     5101 ctccttgcgt cccccctgaa gggcataaac ggctatgcgc cggctcccgg cgtcgatgct
     5161 gttttccacg agctctttga ccaccgccga aggccgttcc accacctctc cggccgcaat
     5221 ctgattgcat agaacatcgg gcaggatgac gatctttccc atcttattct cactttacat
     5281 ttggtgcttg tcgtctcttt ggggtataga acacattcta gaaagcatgg ccggacggcg
     5341 gcgcctctgc cccgaagacg acacgaaaat gaatcagcgg cagccaaccc ggggagaacg
     5401 cacgaatgca gtcgaatcct tatcagtccc tcgtgagtct actcagtaac atactggaag
     5461 cctacacgac agcttttttc ctcctcgatc ccaaaggccg ccaactcaag ctcgccgctt
     5521 cccagtcctt gagcaaattc ctcccccccc gcgtcagcct gcccttggag cagagcggca
     5581 tcctgtccca ggttcacaag gtgggacaga cgatccacct ggacaaggtg cacgaagccg
     5641 cctcgaacct ggcggccacc gttccctttt accgcgaggg agaatcccac atcaaggggc
     5701 tctttgccat tcccgtggga gacggtgccg gggtcctcta cgtggatacc aagtacagct
     5761 gggggtttaa cgacaaacag cagaagtgga tccacgagat cgcggccgtt ctgggagagc
     5821 tggtgagccg ccagaattgc ctggcccagc agaagacgta cgggcgcatc ctggagcttt
     5881 tgcattccct ggaccaagcg accttgaaac agtccgccct cgaggactac tgcaacgagg
     5941 tgatccggga actggccgac tttctggctg ccgagtacgg gttcgtcgcc gttcgggacc
     6001 ccggggaggc gcatcattac ctgtttgcgt gcacgcccaa cgtgcctcgc agcctcacca
     6061 cccagcgatt cctcacgaag caggggctgg tgggttgggt cttccagaac cagaggcctc
     6121 tgctggttac ccgtctgaat ccccagagcc cggatcattt tctgttcacc ccttccgagg
     6181 ctctccccca ccacgggacc ttctggggaa tgcatcttgc cctctccctg ggccacgccg
     6241 tggtgccggc attcatgtcg cgaaccccca cggaatggtc cgtggacgac cagtatgccg
     6301 tttcccacgt cctgcttttt ttgaatcttc tcctggaacg gttctacttt cgggacgagt
     6361 gcgaacacct gcagtgctat gaattctcga cgggcctgta caacgctccc accttcgaat
     6421 cccgaatgga ggcgatcctc aacgcatcca tgcgggacag cacccccttc accctttccc
     6481 tcgtccagtt cgaaccctgg cagctgctgt ggaccaaggc ctcccccaag gaagtgcgtc
     6541 agtaccagat ggagttggcc tcgacggtgt gtgaagccct cccgtccaac accctggtgg
     6601 ggcacctggc ggaaaaccgg ttcggactcc tgtttctcgg ggcatccccc gaagaagccc
     6661 ggcacctgca agctcacctc ttggatttcg atccccgggc cttcgccagg aagaccaagg
     6721 gggttcgcct caaaccctac ctgagtatgg ccgcctttcc ccaggacggg acccgggtgg
     6781 aggagttgtg gcccacggcc tatcagcgtc tgtacgccgc ttttcggaca aaaagtgatt
     6841 ccgcgggcca ataaaggaat tgaccagttg aagcattgtc taatagactc tgacgccgtt
     6901 acagtgagcc gccgtggacc gacagacaag aagggtcatc gatgttagat cgtttgctca
     6961 gttacttctc caaagatctg gccatggact tgggcaccgc caatacgctg atctacctgc
     7021 ggggaaaggg gatcgtgctc aatgaaccct ccgtggtggc catcaatcag gacagccacc
     7081 aggtgatcgc cgtcggtcag gaagcccgga acatcatcgg gcgtcacggg cagcgggtgg
     7141 tgaccattcg gcccctcaaa gacggagtca ttgccgactt tgaagtgacg agcgtcatga
     7201 tcaagtattt tctggccaag atcctcaaga ggcgccagct ggtgcggccc aagctggtgg
     7261 tggccgtccc aacgggcatt accgccgtgg aaaaacgggc cgtcatcgag gcggcggagc
     7321 aggcgggcgc ccgacatatt cacctggtgg aagaacccat ggcggcagcc atcggtgccg
     7381 gtcttcccat cgatcagccc ttcggaaaca tggtggtgga catcggcggc ggcaccacgg
     7441 aggtggcggt gatttccatg tttgccgtgg cgtacagcga atccgtgcgg gtggccgggg
     7501 atgaggccaa cgaggccatt ctccgctaca tgcagcggga acgccagatg atcatcagcg
     7561 aggtcctggc ggagaatgtg aagatgaaga tcgggaccgc cgctccgctc gaagagcccc
     7621 aatccatgga aatcaccggg aaggaaatcc tgacggggat tccccggacc tttaccatca
     7681 cggacgaaga gatccgggaa gccattcggg agccggtgac gaccatcgtg gaatccgtca
     7741 agcgagccct ggaaaaaact cctcccgacc tcgcttcgga catccacgaa aagggtttct
     7801 ggctggcggg aggaggcgcc cttctcaagg gcctcgaccg gcttttggct caaagcacca
     7861 atctcaaggt gaacatcgcc gacgaccccc tcaccgccat cgttcggggt gcgggagccg
     7921 tcattgaaca tctggactac taccgccagg tcttcatcaa ctaggaccgc tcttcgttcc
     7981 taccgacgtc tcccggctcc gggcacaacc gactccaagg tcctcctcca cgaggaccgg
     8041 ttggcctttt caccctccgg aaagcccccc ggggtcatcg atgccctacg ggccctgctg
     8101 ccgttgcttc ttgtgtccct ttccctgtcc tccttgccct tgctgctgag gccgagcctg
     8161 ggaaggctga tgcctcggct gctggggtcg gacctgtgcc ggggaaggct ggtgacgcgg
     8221 ctgcctctga gctccctgcg cctgttgagc cccgctccgg gaccggggtt gagccgcccg
     8281 cggatcttcc cgctgccggg aaccctgcga ttgcatccgg gaggatcgag gccctctgcg
     8341 atcttcccgg aggcccgccc gatctcccct tccgcgatcc ctcccctgca tccgagaccc
     8401 tgcatcccgg gacgggtccg gataccgttg ccgggaatcc ctgcgccgtt ccatttcccg
     8461 ggaaggtcga ccctcgtccc gctcacggga cgaaaccgct cggtccctct cccgacgctt
     8521 gcttccctcc gctccttccc gcgacctcac atccccgtcc cgggaagacg accgggaagc
     8581 ccggtctccc cgctcatcgt ccgacctgac ggaccgcccg gctcgatccc ctcgagacgg
     8641 gcgaaccggg cctctgcggt ccgcgtgatc cccgtcccgg accgagtctt cccccttgcg
     8701 ccggggggaa cgaatcgagg aacgccggga aggatcgccg cggtcctcat cccgacccaa
     8761 tcgatcttcc ttgtctcggc ctccacgatc cgccttcctg gcggactcgc cctcttccct
     8821 ggagggacga accttttccc cacggtccga ggagtcgtcc gatccggagc gctttcccgg
     8881 ttttcccgac cgcttctcct tcctggagac cctgggacct tccttgtccg cctcctcttt
     8941 cgaggctttc ttcttgtcct tgtccgtttg ggcggagggt tgcttgtcct gggaatcacc
     9001 ggtttccgcc tgctttttgg cacccgaacc cggggtgccg gagctgctgg gtcagccgct
     9061 gcccggtcag ccgccgcctc caccgccgga cccgcctccg gaagggccgc taccggtccc
     9121 cttgcccccg ccggtcgagt cgtccgtggg aggcttggga ggcttgggag gccgggtgac
     9181 cttggtcgtg ttcccttccg ggtccaccag gttgacggtc acgccgggag gcacgttgac
     9241 cgtgtggact ccttccccgt cggtgtaggt cagcaccccc gtctcgggat cgaaagcctg
     9301 gcccgtgacc tcgccaaagg agatggaagg cggggggggc ggagaagaat cctccggggg
     9361 gggtgggggt tccttggggc aattggccgc gatggccgcc tggagttttg acatggcatc
     9421 ggccgcctcg gagtcgccgg ggttgtttcg gagataattg gcgagcactc ccacttgcca
     9481 cttggcgtcc gaacacttgg aaggatttcc gctcaccgcc gatgccaggt tgttcgccgt
     9541 caggatcttt tccggccgag tgaacggctg ctggtattca tttgcgttcg tatcatagag
     9601 cacataccac ggaatgccct tcttcttggc gttccgcttc atttcctccc atttttcctc
     9661 cccgtacttt tcgcggccgg cttcgtcgat gagcatctgt cggcgggcgt ccactgccgc
     9721 ctcatattcg tccaacgcct tctgggtctc actgcgcccc tcggcccaca ccggaaaagc
     9781 gttaccccat aataggacca gtaccgggaa ccaaacgaaa cttcccaaac aaatattgat
     9841 ccagcgcttc atgttgcccc tcctcctggg gaaagaccct cccgccaaag ggggcccgct
     9901 ccccaaagcc ttgttgtttg ccgggatttc actcaaacca cgggggccgg aaaagaccgg
     9961 tctcccgccc tgggataggc ccgtctcctt ccggacaacc cgacagtcgg gagaaattgc
    10021 cttgacagct tgaccgccgc tgcgagcgca ccgggggttc gccggtggcg cgaccctctc
    10081 gaacgaagaa ggatcgctcg tggacatctt caggcactca aatcgaagag gaaccgggaa
    10141 cggagtagga agataccggg tccgcaatcc gcaaacacgg caaaaaggac aagatatgtc
    10201 aatgggttga gaagtctacg gaatcccgca cggaccccgg aaggacctca tggggaatcc
    10261 cgggaaggcc cccgcagcat gcccccaaca tgcccggcca ggcaggccgc ttcccccgaa
    10321 cggccctgat ttccccctct ttttcgccgg attacgggag agaaaccagg atgcggggct
    10381 gcagaaccgc acccccgtcc atttgggcca attcaaacat agagtaacaa attcacatca
    10441 aatcaacaaa aacaatattc gctcgcaaaa agaaaactcg gcagcagggg aaaagggcgg
    10501 ccgggcggag gtcggtgagc ggtccggccg gttccgtgcc ctgtgcatac tccgctttcc
    10561 gcactattca gagatcatcc tatggttcga ccctcgccgc gagacagtgg atcaaccgtc
    10621 tcacttggca actctcaacc tacctcgacg agccgtggcc gaacacatcg ggagattggg
    10681 cgatctgttc atcgatgctg cgatcgtagc ggatggcgat cacgcgaaat ccccggctgc
    10741 gcaattcgga tcgtagtacg cgatctctct cggcttgggg cggctcgtcg tggacggacc
    10801 catcgcaaaa gatacaaaca ttagggctgt agaagaaatc ggggatgcaa tggggatcgg
    10861 ggattgcatg ctgggcgtaa tcagggaggc ggtagcctcg tgatgctaag atgtccagga
    10921 aattccgttc gagcacggat cgtgaatccg tgagggatcg cagccacgcc aagtgggaag
    10981 cccagtctcg gccattcact cgaggtcggg tgatgctgga aaccaagtcc agcagatgta
    11041 ccaggattcc gtgccgatcc aggttgatgg cttcgtgctg attgctgaag ctcatgagac
    11101 attcgtagca ggctgcatga cagtcaggac gcaaattgtt tccttcgacg tcaaaatgac
    11161 agcgggtgag agcctcggcg gcgactcggg ccatgccatc ggcttcgtcg acaagccgac
    11221 gcagcacccc ggctccgcct tcgccggctt catacaggag gatggcccgg tgatccccgg
    11281 ttccgattct ctccgccgcc agctccgcct cttcgagctg gaagaactgt tcgcatccgc
    11341 gctgcagcgc gtactggagg gttgactcga gtacgggatc gtttcgcagc tccgggcgtg
    11401 aaaagcgtac aagaagcaag ttctgtgtgc cctggacgca gaggcgtacg agctccggac
    11461 gccgcgggcg tccttgctgg gcacctgttg ccgtgccgcc gctcaacacc tcgccgtttt
    11521 caaagtccac gagaaagccc ggctgcgtag cggagcgcca tccatggttg actcgcatga
    11581 gcgttgccgc aggcgcgtag gtcagacgca aaacgggggt cccaccgcac acgacatccg
    11641 cttcctggat cctcgggttt cctgcggatt cctgagcaaa tcggtacgca acagtcagat
    11701 caaaccctcg tctgcgccgc tcctcttcgt cgcacgtgat tcgatcacgt cccctgcacc
    11761 ggacattggg catttccaat aaagttgcaa tcaggctgtt ggatccatcg aaggttgtcc
    11821 ggcaattggg acacaattcg aatccctggc ccatgtcgca aaacgttcca cagacgttgc
    11881 agaatctttt ttgagtgcgt cgttcatcca agccaccggg cggggcctga aagctcataa
    11941 gctcccactt tcggccttcg tgataaatga tgttcccggg tccgaactcc cgcaaagcca
    12001 ggaatcgcgg ccgagcgatg aattccccct cctcgcgcgg cacccaggcc cgcaccggca
    12061 gggaagggaa gttgtagccc ggaaggaagc cttcactcgc cagatatcga taaggataaa
    12121 agtcgctttc ctcgcgcgtt gtgttgatct gtcgcaatag gttcaattga cgcagcgctt
    12181 cctcctcgcg gcgccgggcg tcatcctgct cctcgcgccg ttgtgcccgg cggaccaggt
    12241 tttgggactc gaccagctgt cggctggcgg ctctgtagag ctcccgccag cgattgaagg
    12301 cccggtcaaa ttcgacgggc gcctgattca agacatgagt gatccactcg tcaccaaacc
    12361 aatcactccc gtcaaggcca cccaaatcca cagccaggac ccgccgagcc cgttcatgta
    12421 gagcataagc ggacgactcg ctcagttgaa tcgtcgccgc tgcatgctcg cgcatgggca
    12481 ggctctcatc aatcgtgtcg atcacctctt cgatggaccg gccgagcggc agccgaacct
    12541 gtgcaagcca cagagcgtgc aagtgggctt tgacgagggc ctcgttggtg aggtccaatc
    12601 gaggcggccg gacgctgccg gcgaccattt cggtgcgctg atggaaaaaa tattggtcat
    12661 gactgttgat ggcgccgcaa tacgtgaata tgaggccggg ttgcccctgg cgcccggcac
    12721 ggccgctgcg ctgggcgtaa ttggcgggtg tgggaggaac gttcc
//
LOCUS       DAOOVC010000108        12765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3241_length_12765_cov_6.016758, whole genome shotgun sequence.
ACCESSION   DAOOVC010000108 DAOOVC010000000
VERSION     DAOOVC010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3241_length_12765_cov_6.016758"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLM79_12820"
     CDS             complement(<1..571)
                     /locus_tag="PLM79_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87241.1"
                     /translation="MNLKGNVPAPGARVLVRDAEWLVRKVDRTSTGGQAVTVVGISEL
                     VRDKEAVFLTEVEDLAKPIEVLDPAKTNLVRDSSSSYQASLLYMESLLRQAPPTDENL
                     YIGHRAAMDPVPYQLVPAVQALRQPRQRILIADAVGLGKTLEAGILLSELIRRGRGKR
                     ILVVVVKSMLTQFQKEMWSRFTIPLTRLDS"
     gene            1742..1814
                     /locus_tag="PLM79_12825"
     tRNA            1742..1814
                     /locus_tag="PLM79_12825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Leu,seq:caa)
     gene            2290..3957
                     /locus_tag="PLM79_12830"
     CDS             2290..3957
                     /locus_tag="PLM79_12830"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HOV87242.1"
                     /translation="MRRGYTIGLVLLFLGWWLSSVDAATYYVRPDGGTAAQCTGQADA
                     PYPGTGVRQACAWAHPFWALDRSGNWKIHGGDTLILAPGNYRMGFGAPNTRWCSRDYP
                     WDCHLPPLPSGPAPDQPTRILGDGWDRGCVKKPRLWGTQRALQVLDLTDTSNAVIECL
                     EITDRSGCALDHCKASVRCPRNDYPFGNYADLGLYAEDSSNVVLRNLDIHGMAWGGIR
                     AGRIKDWLVEDVRLAGNGGVGWDGDIGRRSSNRGTLTFRRMTVEWNGCPESYPGRVPK
                     NCWGQETCGGYGDGMGLARSGGHWVVEDSVFRYNVSDGLDLLYVGVDYPKSLIELRRS
                     MAYGNGGNQFKIGGDSTVVNCLAVGNCGFFYGKSYARKMGPVDSGDHCRAGGGAVAVF
                     LGRGDTASIVNSTIAGQGWALLDAACITKDFPDQPSCNGTESLTLMNNVFQGYQVFYL
                     DYKRLTDFVGDGDPDNFTSSARVDYNIIHKAEVSSPTGPNNLFENPRLENGAIHVFDG
                     HLRSGSPARDSGLPVGSVSGLVPGEDIERNARPVGNGVDRGAYEYGN"
     gene            4532..5053
                     /locus_tag="PLM79_12835"
     CDS             4532..5053
                     /locus_tag="PLM79_12835"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOV87243.1"
                     /translation="MIVRPTATKKRPASPVAYTAVLLAALTLMLGFFRASTATSTGEG
                     TNTMEEVIWKLEEAYFTDLYKAEYEGVLELVHPQFLGWPGNLGKPVGKEEGAAFTKKL
                     VPQPTPCAVRIERGGLQISGDTALTPYTVYVSCSEGSGRMSTQGSRITHTWVMRNGRW
                     QLLGGMSREVKQE"
     gene            complement(5176..5421)
                     /locus_tag="PLM79_12840"
     CDS             complement(5176..5421)
                     /locus_tag="PLM79_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87244.1"
                     /translation="MEIKKRTRKKEKERPCGGMLHDLDRTQKAMKDSGPCGRKAFMAS
                     VGSRSVDDFRKNAGEFPQLPELNSMCQYHSREESSRR"
     gene            5495..7537
                     /locus_tag="PLM79_12845"
     CDS             5495..7537
                     /locus_tag="PLM79_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV87245.1"
                     /translation="MKRGFVLLVPGAAFLLSLFSVVVCAQTTPGQYGGETAEMEAIVV
                     TASKAPKAKSDVTQAVDTLTEEEIDTYIGSKRNIQELLMYQPGFFIDVLSRNDANWGS
                     VGGLPHKYSTFMLDGLPIDSFVDPQSLDPWAFQRIEIQRGPASVLYPNYLFMDFAGNE
                     TPLAGTVNLILRDRIDNPMTKLSAEYGSYNTINGRFYHQNKVGNFHFFLGGQYEQSDY
                     TNYGTDPSWLNMIDDPEYRKIRLYGHGTYFFGNSDEHKLSVFAHNTWHDGDAGRPNRD
                     FEHQYTTLNADYQVPISPCVMARAKVGYRNYDRTWEEDNFPANLALREEDGVKQEIVP
                     GDFSVSFKHLDGGLFTAGTDFQVATYKTFAEVTRKVTGNDATASQNGVYVQEEYKWKD
                     WVFRAGGRYNYTHHDYDRIGGVVPGVDDASWDKLLWSAGVRYNVLESLSFYSNAGTSF
                     VVPGIKSIGGTLKPEDEGVPGKNGQLPNPDLKPESGLGWDFGIDYRATKRLSLGARVF
                     LNKVDDQIVDIVVSQDPSQSKSVNSGKTTAYGVEIEAKHRLNSWLQWFANYTYTNSNV
                     ENPTNPDQDDVEVPFVPEHMGNIGFQAFLPYDIMAAAWLHVAGEIFDSTSKSNRNGFT
                     HYELINAKIQKALVKKEAYKLDCYLDLYNITNNKFTMPWQFQDPGFAASGGLKFVF"
     gene            7796..8833
                     /locus_tag="PLM79_12850"
     CDS             7796..8833
                     /locus_tag="PLM79_12850"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV87246.1"
                     /translation="MNRFSFLRAFLLGIVLVSPGIIPHVCIASELVASSRITVTDFRG
                     KTISLDRPAGRIVCLIESALSGLYMLGAADRVVGISANVYQGDVYPYYAAMDPRIAEK
                     SLPAPGNWDFVNIERVIALDPDLVIIWSRQEESIRAIEERGIPVFGVFIGSFEDIYRE
                     MRALGKLTGTSERAGQLIDAAKEELTRIGAKTQTVGKRPRVYFMWSQGELETSGSKST
                     VHELITLAGGENVCGGIEQEHLVVNMERVLAWDPEVIVLWVNERKGPKDILDSPIWQG
                     VRAVRTQNVHELPDVFSSDLWTLKFQYVVKLLAEWCHPDVYGEADLEKERREIFVKLY
                     GEKILPALSPR"
     gene            8868..9878
                     /locus_tag="PLM79_12855"
     CDS             8868..9878
                     /locus_tag="PLM79_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOV87247.1"
                     /translation="MKRPAIRWILYGLPVPVLALSLLVGPSDVMSPGQAVLWMARRLA
                     GFSGQADAKEALVSAILWNVRLPRVLLTFLVGGALAASGNSIQALFRNPLVCPYLLGL
                     SSGGAFGAALALTTSWIPLQPSAFLFGLLAVTSSYLLARVRGRIATVSLILSGVIVSG
                     IFTSLLTILQFLTDPFKLQTIVHWTMGNLHNATWEKVRSAAPLILASTACLFLMRWRM
                     NVLALGDEEMRAVGLNPEREKLLLILPATLAASASVAVAGVIGLAGLMVPHMVRMMIG
                     PDNTHTLPACFAFGGSFLLLVDNLSRTTASFELPVGVFTTLAGGPFFIFLLKRAGIGW
                     ET"
     gene            9875..10663
                     /locus_tag="PLM79_12860"
     CDS             9875..10663
                     /locus_tag="PLM79_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87248.1"
                     /translation="MNDALRVERLSFRYGKRLVLDDIHLVMREGTFTVLLGKNGSGKS
                     TLLRVMAGLAQCGSGKIELLGKDLRSLSASSRARILGFLPQQHRPVFPFSVEDVVLTG
                     RACHVTLIPGKKDREKAFEAMDRVGIMHLAGRSFTELSGGEQQMVMIARVLAQEPRII
                     LLDEPTAHLDFVNQARLLDLIRELVDSGLTVAAVLHDPNLAFLYGDEFVFLKNGRLQS
                     VSSSQKPWDGSLLNAVYDMDVHTVPYGKRALVIPGGDKKSVDHS"
     gene            11539..>12765
                     /locus_tag="PLM79_12865"
     CDS             11539..>12765
                     /locus_tag="PLM79_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOV87249.1"
                     /translation="MEIPSSKDERSSNPRKTRPVLVMGATGYIGGRLVPLLLELGYTV
                     RVMGRSLPKLESRPWATHPRLEAVQGDVLDIHSLKKAVQGCWAAFYLVHSMNTSTSDF
                     SYLDRVAAWNMVEASGSSTLERIIYLGGLGDESDPALSKHLRSRLEVARILRSGSVPV
                     TTLRAAMILGSGSASFEILRYLVDRLPIMLAPPWVHTPVQPICIRNVLHYLVGCLEHD
                     ETVGQAFDIGGPEILTYEQLIKIYAQAAGLRERVVIPVSFISPRWSPLWISLVTPVPA
                     PLARALTEGLLNKVVCRDERIRRIIPQELMTCRQAIDRAIEKIERHRVEACWTDAGRL
                     LPPEWSYCGDEPYAGGTVLACAYRVRIKASAGEIWEPIVRIGGQTGWYYGGPLWRIRG
                     WLNKLIGGPSLMRGRRD"
BASE COUNT         2546 a         3720 c         3899 g         2600 t
ORIGIN      
        1 cggagtccag ccgggtgagg gggatggtga agcggctcca catctccttc tggaactgag
       61 tgagcatgct cttcaccaca accaccagga tgcgtttccc tcggcccctg cggatgagtt
      121 cactcagaag gatcccggct tccagggtct ttcccagccc cacggcatcg gcgatgagga
      181 tgcgctgcct cggttgcctg agggcctgga cggccgggac cagctggtag gggacggggt
      241 ccatggcggc ccggtggccg atgtagaggt tctcgtccgt tggaggtgcc tggcgaagga
      301 ggctctccat gtagaggaga gacgcctggt aggaacttga ggagtcccga accagattcg
      361 ttttcgccgg gtcgagaact tcgatgggtt ttgccagatc ctcgacctcc gtgaggaata
      421 cggcctcctt gtctctcacc agctccgata tgccgaccac cgtgacggcc tgccccccgg
      481 tggaagttcg atcgaccttg cgcaccagcc attccgcgtc gcgcacgagc accctggctc
      541 ccggcgccgg gacattgcct ttgaggttca tccttgcttc cttcagtttt gctgaatcca
      601 cgcaaagaaa accaatgcca aactccgttt gccgcaaaac ccgtctgggt gcggaaaaag
      661 acccgtcatg gttcatggtt gccgcgacat tggggcgtca ggaatcccag tgcccgcatg
      721 atttccaggt agcggtatag tgaaacaact ccgccattcc agcaagaccg aaaacatccc
      781 gccacggtag gatggagagt catgggtgct gatcggggtg tagcggccca agaaaaggtg
      841 gccgtccgca tggagaatgt accgaaaggg tatcatcgtt tccatgatgt cgacttcaac
      901 ggtttcaaca tcgactaggt cgccgcggga cctggtggaa tctttcgcat cgaaaccaag
      961 agccacaggg gaggtatcgc ttcgaacggt gacaatctac tgctgaacgg caagactccc
     1021 gtacccggac gcaaacgaag gatcgtgaac gattcttccg gaaacagaca tccaaagaat
     1081 ggagggtcaa gcctccctct gcttcgagaa tgcttacgcg aggattcgtg aaccggtgaa
     1141 tgccatcacc atgtccggta tcggctaccg gaatggattc ctctttcggc agccccatat
     1201 gtcatcaagc gggaagatgt ggagcggatt gcccgcattc tttcttggtg aatttcaagg
     1261 aatcggcgca aaggaaggca gccgtcgggc cgacttcttg tcgtggcatg gcatactgcg
     1321 ttcacccttc aattgcccac ggtttcgtgg tatagaccta ggcacctacg gcagccgttc
     1381 atccgatgtc gagttacacg attccgagag catcggggct tcatgcccgc gaaggcgggc
     1441 atccaacggg taggcaccct ctttccgaat tcccgcttgc gtgggaatga cgaaagggag
     1501 ttccgaccgc ccaaatcgct ccatccgggc tggtgctgtg ggggtgtcca agatacactg
     1561 tgaagagcag catacccctc tggtctcgca atcgcacagt tcgctctccg tcaggcaaac
     1621 cggcggaacc acacctgatg gccccgtaag agttcgttct tctcgggacg acacctcatg
     1681 aattcaatcg gttagagccg aagaaacggg ctcttcagga ctttttgcga aatcgtccat
     1741 gattgcaata ggttgttcgt cgtcgggggc tcatgacccg aaggtcggag gttcaaatcc
     1801 tccctgcgct gccatgagca aaccggggaa ctcgggggcg gaccttcggt cccttttcct
     1861 gggggacttt cgttctccag ccttcacacg gacggttgga acttccaacc gatcccctcg
     1921 aaccggccgc gagggaagaa ccgtgatgaa gtcgtgcgcc tcggtaggcg gcgcaccatc
     1981 gaggggtgct gggacggggg cgggaatgga tcgggattct ccttcgaacg tgagatccga
     2041 gagtgttcgt ttcgacggaa tacgtcgccc tccgccttcc ggttgcccgt cctcgtccga
     2101 ttgacgcgga gttcgccggt tttatgggaa gcgccccggg aatggacagg aatccacatc
     2161 gcaacgcggc tcatgggatt ggtcccggct gccgtcgccg ttgaggtcgt tctcggcggg
     2221 gtggcggtcg tttttcgcgg ctttgtccaa cggcaagggt cccatgggtc tggagaagga
     2281 gggagagcca tgaggagagg gtacacgatc ggcttggttc tgttgttcct tggctggtgg
     2341 ctgtcgagtg ttgatgcggc cacctactac gtgagaccgg acgggggcac ggccgcccag
     2401 tgcacgggtc aggccgacgc gccgtatccc ggcacgggag ttcgacaggc gtgcgcctgg
     2461 gcacatccct tctgggccct ggaccgctcg ggaaactgga agatccatgg gggcgacacc
     2521 ctcatcctgg cgccaggcaa ttacagaatg ggcttcggag cccccaacac ccgttggtgc
     2581 tcccgggatt atccctggga ctgccacctc ccgcccctgc cttcgggccc cgccccggac
     2641 cagccgacca gaatcctggg ggacggctgg gaccggggct gcgtcaagaa accccggctc
     2701 tgggggaccc aacgagccct ccaggtgttg gatctgacgg acacctccaa cgccgtgatc
     2761 gagtgtctgg agatcaccga ccgctccggc tgcgcactgg atcactgcaa agcatccgtc
     2821 cggtgtccca gaaacgacta cccgttcggc aactatgcag acctggggct ttatgcggag
     2881 gactcttcca acgtggtgtt gagaaacctg gacatccacg gtatggcatg gggcggcatt
     2941 cgggcgggtc ggatcaagga ctggcttgtc gaggacgtac gcctcgcagg caacggggga
     3001 gtggggtggg atggagatat cggccgacgc agctccaatc ggggtaccct gacctttcga
     3061 aggatgaccg ttgaatggaa cggatgcccc gaatcctacc ccggccgggt ccccaaaaac
     3121 tgctggggcc aggagacctg cggcgggtat ggggacggca tgggactggc ccgctccggg
     3181 ggacattggg tggtcgagga ttccgtgttc cgatacaacg tctccgacgg actcgacctt
     3241 ctctacgtgg gggtcgatta tcccaagtcc ctgatcgagt tgagaaggtc catggcttac
     3301 gggaacggag gaaaccagtt caaaatcggg ggggattcga ccgtcgtcaa ctgccttgcc
     3361 gtgggcaatt gcgggttctt ctacggaaag tcctatgccc gaaagatggg tcccgtggac
     3421 tccggcgatc attgccgtgc cgggggaggg gccgttgccg tctttctggg ccggggagac
     3481 accgcatcca tcgtcaattc caccattgca ggccaaggtt gggcactgtt ggacgccgcc
     3541 tgcatcacca aggactttcc ggaccagccg tcctgcaacg gaacggagtc gctcaccctc
     3601 atgaacaatg tgtttcaggg ctaccaggtc ttctacctcg attacaaacg actgacggat
     3661 ttcgtggggg atggagatcc ggataacttc acatcgtctg cccgggtcga ctacaacatc
     3721 atccacaagg cggaagtttc gagtcctacg ggccccaaca acctgttcga gaacccacgg
     3781 ttggagaacg gcgcaattca tgttttcgac gggcacctgc ggtccggcag cccggcccgg
     3841 gattccgggt tgcccgtggg cagcgtttcc gggttggttc ccggggaaga catcgagagg
     3901 aatgcccggc ctgtgggcaa cggagtggac cggggcgcct acgagtacgg gaactgatcg
     3961 ccgtttcggg cgggagggag cgcctccacg ggaaccagca acccgatcga gacgcctgcg
     4021 ggcttccggc gaaaagtctg cccacccccc attgggattg ggaggtcggg agcgaggggg
     4081 aatctctccc ctcgttcccg aaccggcacc ccatcttctc gataagcgcc ccctcggcga
     4141 actgctcttc gatcttggcc gcaagccgcg ttgtcctctc gtcgaacggc tcgccgtcat
     4201 cctgctgtgc ctcggcgcag atgcagcggc gaccctttgc cgccccgcgc ttccccaatg
     4261 ggaaacggac gtcggcacgg accggtctcg atgttaccat gggtcttgtg gcgcgttgaa
     4321 ctccgagctt ttgactcccg gatctttggc cgtgtatctg aagactcccg gacgtcaccc
     4381 gtcggatgga agccaaagcg gctccgcccc gggagaattc aaagctcacg gctgaaggct
     4441 aatcgcttgt ggataagacc caaccggtac atccggcgga ggactttcag ccgctgctga
     4501 tccgcaggtt gggccgacat gaggagggaa cgtgatcgtt cggccgacag cgacgaagaa
     4561 gagacccgct tcccctgttg cctatacggc agtgctgctt gcggcactga ctctgatgct
     4621 cgggtttttc cgggcatcca cggcaacgag cacaggagaa gggacaaata ccatggagga
     4681 agtcatctgg aaactggaag aggcatactt cacggatttg tacaaagccg agtacgaagg
     4741 tgttttggag cttgtgcatc cgcagtttct cggatggccc ggcaacctgg gtaagcccgt
     4801 cggcaaagaa gagggcgcgg cgttcacgaa gaagttggtc ccacagccaa cgccgtgcgc
     4861 cgtcaggatt gaacgtggtg gtctgcagat atcgggagat acggcattga ctccgtatac
     4921 cgtatacgtg agctgttccg aggggagcgg aaggatgagc acgcaggggt cgcggatcac
     4981 ccacacgtgg gttatgagaa acgggcgatg gcagctactg ggcggtatga gtcgcgaggt
     5041 caagcaggag taggtcggag gaaagtcccc gacctgagat cgtgcgggtt gcttcgcgtc
     5101 cggtgatttg ggcacccgcc ctcttcctat cctccgggct gccctctcca tgactccttg
     5161 ctcctatttt tgaaactatc ttcttgaaga ttcctcgcgg ctgtggtact ggcacatcga
     5221 attcagctcc ggaagctgag gaaactctcc ggcgtttttc ctgaaatcat cgacacttcg
     5281 acttccaacc gaagccatga aggctttacg accgcaaggg ccggaatcct tcatggcttt
     5341 ttgtgttcta tccaggtcat gaagcatccc cccgcaaggt cgctcttttt ccttctttct
     5401 tgtccgtttt ttgatttcca ttcatccggt accgctttcg gaagggccac tccggagcgg
     5461 gcgcggcttg tatgggagaa aggagcagag taagatgaag agagggtttg tgcttcttgt
     5521 tccgggggcg gcatttttgc tttcactctt ttccgtcgtg gtgtgcgccc agacaacacc
     5581 gggtcagtac ggcggtgaga cggcggagat ggaggccatt gtggtaacgg catccaaggc
     5641 gccgaaggct aaatcggatg taacacaggc tgtggatacg cttaccgagg aggaaatcga
     5701 tacctacatc ggcagcaaaa ggaacattca ggagctgctt atgtatcagc cgggtttttt
     5761 catcgacgtg ctgtcgcgaa acgacgccaa ctggggctcg gtgggggggc tcccgcacaa
     5821 atacagtacc ttcatgctcg acgggcttcc aatcgactcc ttcgtcgatc ctcagagcct
     5881 ggatccctgg gctttccaga ggatcgaaat ccagcgcggc ccggcgtcgg tgctctaccc
     5941 gaactatctc ttcatggatt tcgcgggaaa cgaaacgccc cttgccggaa cggtgaacct
     6001 gatcctgcgg gaccgaatcg acaatcccat gacgaagctc tccgcggagt acggctcata
     6061 caacaccatc aatgggcgct tctatcacca gaacaaggtg ggcaacttcc acttcttcct
     6121 gggcggccaa tatgagcagt cggactacac gaactacggc accgaccctt cctggctgaa
     6181 catgatcgac gacccggaat accggaaaat caggctctac ggccatggga cttacttttt
     6241 cggcaactcc gacgagcata agctttcggt gtttgcgcac aatacctggc acgacggtga
     6301 cgcggggcga cccaaccgcg atttcgagca ccagtacacg accctcaacg ccgactatca
     6361 agtccccata agcccctgcg tcatggcccg cgccaaggtc ggctatcgca actacgaccg
     6421 cacctgggag gaagacaact tccccgcgaa cctcgccttg cgggaggaag acggcgtcaa
     6481 gcaggagatc gttccaggag acttctcggt atcgttcaag cacctcgacg gaggcttgtt
     6541 caccgccggc accgatttcc aggttgccac ctacaagacc tttgccgaag tgactcggaa
     6601 ggttaccggc aacgatgcca cggctagcca gaacggagta tacgtgcagg aggagtacaa
     6661 gtggaaagac tgggtgttcc gggcgggagg ccgatacaac tacacgcatc atgactatga
     6721 ccgcattggc ggggtggtgc ccggagttga tgatgcatcc tgggacaagc tgctgtggag
     6781 tgcgggtgtg cgttacaatg tcctcgagtc tctgtctttt tactccaatg caggcacaag
     6841 cttcgtggtt cccgggatca agtccatcgg cggcacgctc aagcccgagg acgagggcgt
     6901 gcccggcaag aacgggcagc ttcccaatcc cgacctgaag cccgaaagcg gactcggttg
     6961 ggatttcggc atagactacc gggccacaaa acgcctgtcc ctgggtgctc gagttttcct
     7021 caacaaggtg gacgatcaga tcgtggatat cgttgtgagc caggacccct ctcaatccaa
     7081 gtccgtcaat tcggggaaga cgaccgccta tggcgtcgag atcgaggcca agcacaggct
     7141 gaattcctgg ctacagtggt tcgccaacta cacgtacacc aacagcaacg tggaaaaccc
     7201 gacaaacccc gaccaggacg acgtcgaagt tcccttcgtt ccggagcaca tgggaaacat
     7261 aggctttcag gctttccttc cgtacgacat aatggcagca gcgtggcttc acgtggcggg
     7321 ggagatcttc gacagcacat ccaaatcaaa caggaacggg ttcacccact acgagctcat
     7381 caacgcgaag attcaaaagg cgttggtcaa gaaggaggcc tacaagctgg actgctatct
     7441 cgatctctac aacatcacca acaacaagtt cacgatgccg tggcagttcc aggacccggg
     7501 gtttgccgcg tcgggtggac tgaagtttgt gttctagtgc gatgtcaaag gtgtcctacc
     7561 gggtccgcga cgggcggcag ggtgggcaaa agcagagggt tgccggtccc ggatcgcaca
     7621 acggaggcgg ataatactgc gcccacccta cgatttcaac cgggcgaagc tgaaatgacg
     7681 gagcacccgc agcgaacggt gtagggtgaa atccggacag tgggaggcag accggcggac
     7741 ggcgattcat atccgctctt tcccgctcac cgttccctcc cctggaagga cagcgatgaa
     7801 tcggttctct tttcttcggg cgtttctgct ggggatcgtt cttgtttcac cgggaatcat
     7861 tccccacgtg tgcattgcgt ccgagttggt cgcatcctcg aggatcacgg tgacggattt
     7921 tcgcgggaag acgatttccc tggaccgccc cgccgggagg atcgtctgcc ttatcgagag
     7981 cgccctcagc ggactgtaca tgttgggggc cgcagaccgc gtggtcggca tctccgccaa
     8041 tgtctaccag ggtgacgttt atccctacta cgccgccatg gacccgagaa ttgccgaaaa
     8101 gagcctccct gctccgggga attgggattt cgtcaacatc gagcgggtga ttgccctcga
     8161 tcccgatctc gtcatcatct ggtcccgtca ggaagagtcc atacgagcca ttgaggagcg
     8221 gggcatcccg gtgttcgggg tctttatcgg gtcattcgag gacatatacc gggagatgcg
     8281 cgccctggga aagctgacgg ggacctcgga gcgggcaggg caactgatcg acgccgcaaa
     8341 agaagagctt acgcggatcg gagcgaaaac acaaaccgtc ggcaagcgtc cgagggtcta
     8401 tttcatgtgg tctcagggag agctcgagac atcgggaagc aagagcaccg tgcacgaact
     8461 gatcaccctg gcgggaggcg agaacgtgtg cggaggcatc gaacaggagc acctggtggt
     8521 gaacatggag agggtcctgg cgtgggaccc cgaggtcata gtcctgtggg tcaacgaaag
     8581 aaaaggacca aaggacatcc tcgatagccc tatatggcag ggcgtgcggg cggtgcggac
     8641 tcaaaacgtc cacgagcttc cggatgtctt ttcaagcgat ctttggaccc tcaagttcca
     8701 gtatgtagta aagctgctgg ccgaatggtg ccacccggac gtctacggtg aagcggacct
     8761 ggagaaggaa aggcgggaga tcttcgtgaa gctctacggt gagaagatct tgcctgccct
     8821 ttccccccgg taaaccagtt cgactcgcaa cccccgagag gtgtcctttg aaacgtcccg
     8881 cgatccgttg gattctctac ggccttcctg ttcccgtgct ggccctctcc cttcttgtgg
     8941 gaccctccga cgtgatgtct cccggtcagg cggttttgtg gatggcgcgt aggcttgcgg
     9001 gattctccgg gcaggcggac gcaaaagaag cgctcgtgag cgccatcctc tggaacgtgc
     9061 gtcttccgag ggtccttctc acctttctgg tagggggcgc cctggctgcg tccggcaaca
     9121 gcatccaggc tctctttcgc aatccgctgg tttgccccta tctcttggga ctttcttcgg
     9181 ggggcgcctt tggagcggca ttggcgctca ccacttcctg gatacccctc cagccctcgg
     9241 ctttcctctt cgggctgctc gccgttacat cgagctacct tcttgcccgc gtcagggggc
     9301 ggattgcgac ggtttctctc attctctccg gagtcatcgt ctccgggatc ttcacctcgc
     9361 ttcttaccat ccttcagttc ttgaccgacc ccttcaagct ccagaccatc gtgcactgga
     9421 ccatgggaaa cctccacaac gccacctggg agaaggtacg ctcggcagcc cctctcattc
     9481 ttgcaagcac ggcatgtctc ttccttatgc gatggcgcat gaacgtcctt gccctcggag
     9541 acgaggaaat gcgggcggtg ggactcaatc ccgagcggga aaaattgctg ctcatcctgc
     9601 cggcaacgct ggccgcatcc gcctccgtgg ccgtcgccgg cgtcatcggc ctggcaggtc
     9661 tgatggtgcc tcacatggtg cggatgatga ttgggccgga caatacgcac accttgcccg
     9721 catgctttgc tttcggggga tctttcctcc tcctcgtgga caacctgtcg agaacaacgg
     9781 cttccttcga actccccgtg ggggtgttca cgacgctggc gggcggcccc tttttcatct
     9841 ttcttttgaa aagggccggg atcggatggg aaacatgaat gacgccctac gagttgaacg
     9901 cctgtccttc cgctacggca aaagattggt ccttgacgac attcacctgg tcatgcggga
     9961 aggaacattc accgtgctcc tcgggaagaa cgggagcggc aagtccacgc tccttcgcgt
    10021 catggccggc ctggcccaat gtgggagcgg caagatagag cttctgggga aggaccttcg
    10081 gagcctttcc gcctcgagtc gggcaaggat tttagggttc ctgccgcagc agcaccggcc
    10141 cgtctttccc ttttccgtgg aagatgtggt cttgacgggg cgggcatgcc atgtgacgtt
    10201 gattccagga aaaaaggacc gtgaaaaggc gtttgaagcc atggacaggg taggaatcat
    10261 gcaccttgcg ggacgatcct ttacggagct ctccggcggc gagcaacaga tggtcatgat
    10321 cgcccgcgtg ctggcccagg agccccgcat catccttctt gacgagccaa ccgcccatct
    10381 cgattttgtc aaccaggccc gactgctgga tctcatcagg gaactggtgg attcgggact
    10441 cactgtggcg gccgtcctgc acgaccccaa cctcgccttc ctctacggcg atgaattcgt
    10501 atttctgaaa aacgggcggc tgcagtccgt gagcagctcc caaaagccgt gggacggaag
    10561 ccttctcaat gcggtctatg acatggacgt tcataccgtt ccctatggca agcgcgcatt
    10621 ggtcattccc ggaggggata agaaatccgt tgaccactct tgaccggatg cctgcgccgt
    10681 cattcccctc cgggccgatt gttgtccaca aacagaaatg tcctcttttg ccggaaatcc
    10741 ccgaaagcga caaaccacaa gtcggaaggt caacgacatc gagacgcctg attcagggaa
    10801 ttggcgacaa ccgtgtcgtt cgggctaccg cattgcggtg ccttgcgggt catgccggtg
    10861 aggacacgga ctcgaaaaag accctccata gcccgatggg gccgggggta acggaaggtc
    10921 ccccatcgaa aatcaccggt gcccgtgagg gtcgtcctga gggtttcgcg gcccgcaagt
    10981 ctcgaaacat gcgaaggggt taggggcagc ggggtatcct ttgtgggggc ggcatccggt
    11041 cccgcttgtc tgccgcacgg ccgggtcttt cttccataaa ttgatggtcc cgtaaaagtc
    11101 tctttcttgg aacggcaccg aaggaattca atcggttagg gtcgaggaga cgcgctctcc
    11161 ggggcctttt cccaaaccat cataaattgg aatgcgtctc ttcgacagcc gttttgccca
    11221 gtgccgggtc atcgacccgg gagcggaccg ccgttgggcg acggcaaccg gaagcgtgcc
    11281 tgaaatccgg ggcgacggcg gcgcattcgc tgttgggggg gggaactctt ccttcccgtg
    11341 cttccccaat ggaaaatagc cgtcggcatg gaccggtttc aatgttacta taaagttacg
    11401 caggattcgt gcagatcact tgtggatggg ccctgaaggt tcacgggggg ggggacgttc
    11461 gagatggaga ctgctttccg gcaggagact ccgggcctgg gcaaagaagc aaggcgcggt
    11521 ggagagggga cattcgagat ggagattcct tcctcgaagg atgagcgcag ctccaaccct
    11581 agaaagacca gacccgtcct ggtcatgggc gcaacgggat acatcggggg ccgcctggtg
    11641 ccgttgcttc tcgagttggg atacacggtg cgggtgatgg gacggtcttt gcccaagctg
    11701 gaatcgcggc cctgggcaac tcatccgagg ctcgaagcgg tgcaggggga cgtgctggac
    11761 atccactccc tcaagaaggc ggttcaaggg tgttgggcgg ctttttacct ggtccactcc
    11821 atgaacacgt ccaccagtga tttcagctac ttggaccggg ttgccgcctg gaacatggtg
    11881 gaagcctcgg gcagttcgac cctcgagcgc atcatttacc tcggggggtt gggggatgag
    11941 agcgacccgg ccctgagcaa gcacctccgt tcgcgcctcg aagtggcccg aattctgcgc
    12001 tccgggtccg tcccggtcac gaccctgcgg gccgcgatga tcctggggtc cggaagcgcc
    12061 tccttcgaga tcctgcggta cctcgtcgac cggctcccca tcatgctcgc acccccctgg
    12121 gtgcacacgc ccgtccagcc catctgcatc cgaaacgtcc tccattacct ggtgggctgc
    12181 ctggaacacg atgaaaccgt cgggcaggcc ttcgatatcg gaggacccga gatcctcacg
    12241 tacgaacaac tgatcaagat ctatgcccag gcagccgggt tgcgggaaag ggtggtgatt
    12301 cccgtttctt tcatatcccc tcggtggagc cccctttgga tctccctggt gaccccggta
    12361 cccgcgcccc tggcacgagc cctgaccgaa gggctcctga acaaggtggt ttgccgcgac
    12421 gaacgcatac gccggatcat tccccaggag ttgatgacct gccgacaggc catcgatcgc
    12481 gccatcgaaa agatcgagcg gcatcgggtc gaggcctgct ggacggatgc gggccgactc
    12541 ctccctcccg aatggagcta ctgcggagat gagccttacg cggggggaac ggttttggcc
    12601 tgtgcctatc gggtgcggat caaggcatcc gccggggaaa tctgggagcc catcgtccgg
    12661 atcggaggcc aaacgggctg gtactacggc gggcccctgt ggcgaatccg cgggtggttg
    12721 aacaagctca tcggcgggcc cagcctaatg cggggaaggc gggat
//
LOCUS       DAOOVC010000109        12706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3258_length_12706_cov_7.106790, whole genome shotgun sequence.
ACCESSION   DAOOVC010000109 DAOOVC010000000
VERSION     DAOOVC010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12706
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3258_length_12706_cov_7.106790"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="groEL"
                     /locus_tag="PLM79_12870"
     CDS             complement(<1..668)
                     /gene="groEL"
                     /locus_tag="PLM79_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV87250.1"
                     /translation="MAAKEIKYDAEARDRIMNGVSILADAVKVTLGPKGRNVVIDKSF
                     GAPLVTKDGVTVAKEIELADKFENMGAQMVKEVASKTSDVAGDGTTTATILAQAIYRE
                     GSKLVAAGHNPMALKRGIEKAVEKAVAELKNLSKPTKDQKEISQVGTISANNDPTIGN
                     IIAEAMSKVGKEGVITVEEAKSMETTLEVVEGMQFDRGYISPYFVTDPERMETVLEEP
                     YLLV"
     gene            complement(722..1009)
                     /gene="groES"
                     /locus_tag="PLM79_12875"
     CDS             complement(722..1009)
                     /gene="groES"
                     /locus_tag="PLM79_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOV87251.1"
                     /translation="MKLKPLSDRIVVKRMEEEEKTAGGIIIPDTAKEKPQQGKVVAVG
                     DGKYSEDGKRTPLTVQEGDRILFSKYAGNEIKIEGEDLLIMREDDVLAIVQ"
     gene            1434..2111
                     /locus_tag="PLM79_12880"
     CDS             1434..2111
                     /locus_tag="PLM79_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOV87252.1"
                     /translation="MQHGSSNTIRQARPTRREFAVFTLLTFLMIGSSAWAVRSLPSSG
                     VVTAVFDGDTILLDTRDRVRYLGIDAPEVSHGKEASDCMGEEAAKFHSQMVLHKRVFL
                     HYDRERRDDHGRLLAYVHLPDGRCANVELLRGGYAFVRRSREGFARLNEFLGIQREAV
                     QARRGWWSKCPGTPAGEYLGNRRSFVLHVPHCPWGKRTEARNRERFPSRWRAFEEGYR
                     PCRECKP"
     gene            2137..2598
                     /locus_tag="PLM79_12885"
     CDS             2137..2598
                     /locus_tag="PLM79_12885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV87253.1"
                     /translation="MNRRYPDRPMVGVGAIIFRGREVLLVQRGREPSYGKWSIPGGLV
                     ELGENLRDAVEREVREESGLEVEVEDLAVTLDRVILDENRRIEYHYVLLDFLCRWKGG
                     EPRAATDVLRCEFVSLDALSGYSLTQGTEEAVRRVYRLSLGERQKIYDPDL"
     gene            2745..3668
                     /locus_tag="PLM79_12890"
     CDS             2745..3668
                     /locus_tag="PLM79_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOV87254.1"
                     /translation="MQHLGRIVWKKVAQGTDDPEAIGCLLERHISLDQPRKVLLNELV
                     SAFTELGQSLKHDPPALPPSLPRIIQGFQQFARKAEIPPDELCSSLIEGMILHAHHED
                     GTRTAPKPSTQEKILDAALEVFSHKGFHVATVDEIAERAGVGKGTLYRYFSNKETLFN
                     ELVRLRLEELERDANAALNDRDNVLTMIAKYLRVYFEFFDRNQRLYRVIVQERLDVGK
                     QVQDLYVKKVMRRLPALKRKVYEASQQGVFKDVDFQTVFYGVMGFSHGVIQKWLAREC
                     SYSLVEELPTVLEVLFYGFVKDSQSLPKRVP"
     gene            3686..3925
                     /gene="acpP"
                     /locus_tag="PLM79_12895"
     CDS             3686..3925
                     /gene="acpP"
                     /locus_tag="PLM79_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOV87255.1"
                     /translation="MNAMRNKVVDIIANQLGVDKEIVTPEANVVDDLGADSLDVVELV
                     MALEEAFDLEIPDEEAEKIRTVKDIFEYLEKSLQA"
     gene            complement(4064..4264)
                     /locus_tag="PLM79_12900"
     CDS             complement(4064..4264)
                     /locus_tag="PLM79_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87256.1"
                     /translation="MNPDDEKVLRIAKEIVVKFIELGRVSPGNFDAHFRSVFWTVKDT
                     VVNAQAPDWSSELSKPGETGEE"
     gene            4584..5045
                     /gene="mraZ"
                     /locus_tag="PLM79_12905"
     CDS             4584..5045
                     /gene="mraZ"
                     /locus_tag="PLM79_12905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOV87257.1"
                     /translation="MVVLEKSYFRGQSVARLDAKGRLRIPAKFREVLQHRQSDSVIIT
                     WLDKCLVVYPIDYWQQIEAKVLGLSQIQPQHRAFVRQFISGAEECPFDGQGRVLIPPL
                     LREKAQLGQEVLLAGLLTNFEIWNKGAWDQQNDWGREHFREIALDIAHTGF"
     gene            5052..5996
                     /gene="rsmH"
                     /locus_tag="PLM79_12910"
     CDS             5052..5996
                     /gene="rsmH"
                     /locus_tag="PLM79_12910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOV87258.1"
                     /translation="METKSPVGHVPVLGKAAVDLLGCSPGKLYVDGTVGGGGYTEAIL
                     QRSAPDGRVLGLDWDEEAVERVRERFSHEPRLVLRKAGFSDLHAILEEMHWGPVHGIV
                     IDLGVSTFQLDDPRRGFSFLRQGPLDMRMDLGISETAADLVNSLSQEDLANLIFRLGE
                     ERYARPIARAIVTRRRRRPFTGTLDLAAVIETVVPRTRDTLRIHPATRTFQALRMAVN
                     QELESLEAFLSRVLGDLRSGGRLCVVSFHSLEDRMVKDRFRSWARSCRCPEDLPVCRC
                     EGRPLGKLLTRKAVRPDPEEVKRNPRARSARLRAVEKI"
     gene            6009..6338
                     /locus_tag="PLM79_12915"
     CDS             6009..6338
                     /locus_tag="PLM79_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87259.1"
                     /translation="MHTLKLSPELLSREKADSGTGLWKWLVVVGTLAAVFACGAVFYV
                     WLTIERVQNGYRLARLHDEYEQLLSVQRKLRLEWVRLQDPFHMEEVGRIQLGLVPPRP
                     DQKISLR"
     gene            6436..8430
                     /locus_tag="PLM79_12920"
     CDS             6436..8430
                     /locus_tag="PLM79_12920"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOV87260.1"
                     /translation="MDARNGRNFLSARYWSCYAFCNVLVLSVLCLILFKALRLQVWEH
                     QVWLQRERAQMETTFQVPTYRGSIYDRQGRLLAYSVPQHSLYADCEMVENSAQLATRL
                     APILGEPEATLEKRLASGRKFVWLKRHLTDQQALAVEKLRARGLNLIQEYRRFYPHRQ
                     IAGHVVGFVGRDGTGLEGIEKSFDRVLRQSTTTFSQHRDGVRKYLWLNADPPPEIKEN
                     FGVTLTIDAYLQYLCESELEKCVQKYRAASGEVVVMDPDTFEILAMANWPSFDPNLEG
                     KKNAEIWRNRSITDFFEPGSTFKVFLVSAALEEGSVREKDRVFCENGKCVLAGHMVND
                     VHPYGWLTIPEVIKYSSNIAAGKLALQLGGERYHRHIKGFGFGSRTGVELPGEARGLV
                     RSWKKWRPIDLATTGFGQSIGVTALQLTAGIGCIANGGEWKQSRVVLGIVDSDSRPTE
                     PLRTKPTRRTIQSKTAQQIRDMMQSVTQEGGTGVNAAPEGYSAAGKTGTAQMLDPATK
                     RYASNKYTSLFTGFVPAEKPRLVISVVIHEPHGAIYGGVVAAPVFKNVAAKALPYLGV
                     SPSPGRTGNGPKLKLVNAPKKESTKSPPAVENAASSKMPDVSGLSLQEALQRLAPLDA
                     QVKLRGRGTVVSQSPEVGTPLKSKGTVELVLNDVRETGTR"
     gene            8492..10024
                     /locus_tag="PLM79_12925"
     CDS             8492..10024
                     /locus_tag="PLM79_12925"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOV87261.1"
                     /translation="MQKALGTLLDGLEVLDARGNIGTPIGGVAYDSRQVSRGDLFVAV
                     RGTRQDGGAFIRDAVQRGAQAIVAESEPENGVALPWIRVPDSRRALARLSANFFDHPS
                     RGLNLIGVTGTNGKTTTTLLLEGILAQGGHGVGVLGTLGYRWPGTRRAAPMTTPESLD
                     LQRLLHEMRGAGVTHVVMEVSSHSLALGRVEGCFFKAGVFTNLSRDHLDFHGSMEEYF
                     AAKSLLFTQFLSSGRERGASVINLDDPYGERLARSVAGEIWTYSVDRPEATVRVRGAD
                     LGTRGIRAELITPAGLLKIESPLLGRLNLYNLLSAASAALSVGIPGEAVRSGLAEIHS
                     VDGRLQQVAVPSRLDFQVVVDYAHTPDAMEKSLSCLREMTRGRLVAVFGCGGDRDRGK
                     RPLMGKTAARWADLVVITSDNPRTEVPEEIIREIEPGVREEGMPYLDPDREDLPARGY
                     SVVADRRKGIELALSWARAGDVIFIGGKGHETYQIVGTTVLPFDDRVVVREFLGSGEE
                     SS"
     gene            10046..11440
                     /gene="murF"
                     /locus_tag="PLM79_12930"
     CDS             10046..11440
                     /gene="murF"
                     /locus_tag="PLM79_12930"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOV87262.1"
                     /translation="MNWTRSDILEATRGTLLQGSPVGSVGRISTDTRSIEPGDAFVAL
                     RGENHDAHDFLGAAVEKGAETLVVSRLPDERPVPAPSHVTVVRVPDTLTALGQLARFH
                     RSRFTVPVAGITGSNGKTSTKEMVSAILGQGRKVLKNRGNFNNLVGVPLTLLSLEPDH
                     EIAVVEMGINVPGEMDRLVHMAVPTAGIVTNVHPAHLEGLGSLDRILEEKGKLLSSLG
                     AGGVAVINLDDERLETFSKRLECRRVTYSLQKQEAMVALGGPVEVKEGVSVFPLRLGK
                     ETVPARLSVLGMHQVQNAVAAAALAWALGESPESVVRGLSLHQPVKQRMQMHRLAGER
                     ILIDDTYNANPQSMLASVRTVVAASSGRPVLAVLGEMRELGPGSAALHYEVGFQVGAS
                     GIRFLITMGEFGREIQRGARDAGLSASSCFHAGSHEEAVERLRDWMPRDAWIVVKGSR
                     GMTMERVVEGMMDS"
     gene            11437..>12706
                     /gene="murD"
                     /locus_tag="PLM79_12935"
     CDS             11437..>12706
                     /gene="murD"
                     /locus_tag="PLM79_12935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOV87263.1"
                     /translation="MKAMRPGAAMPKKALVVGLGISGRSVCELLFRHGVRVTATDLKR
                     REEFGGMLDSLEAKGCGLRLGTHRVEDFLEADQIIVSPGIPLEIEPLRKAAQKGIEIV
                     GELEWAWRMTTTPVIAVTGTNGKTTTTSLIGEMLRRAGKQVFVGGNIGTPLSEWVASG
                     READFLVLEVSSFQLDTAPRFCPEIGILLNVTEDHLDRYDGFSSYADSKLSLFKRQTS
                     SHLAILNGDDPVCTARAGDLPGRVLFYSRNDSTAHAVLRNREVRVRLPGGEDYLFSLK
                     GSSLRGVHNEENILAAGLVAASLGIPREVLEQTVRQYRVLPHRLQWVRTWMGIDFYDD
                     SKGTNVGAVVKALENFRQPVLLLLGGKDKLGSYSPIGERMKRLGKAVYAFGEAAPRMV
                     QELDPWVSVRSFANLPEAFREAVKDARSGDV"
BASE COUNT         2489 a         3668 c         4054 g         2495 t
ORIGIN      
        1 cagacaagga ggtagggctc ctcgagaacc gtttccatgc gttcgggatc cgtcacaaaa
       61 tagggagaaa tgtagccccg gtcgaattgc atgccctcaa ccacctcgag ggtcgtctcc
      121 atgctcttgg cttcttccac cgtgatgacg ccctccttgc ccactttgct catggcctcg
      181 gcaatgatgt ttccgatggt ggggtcgttg ttggcactga tggtgccgac ctgggagatt
      241 tctttctggt ctttggtggg tttgctcagg ttcttcaatt ccgcaaccgc tttttccacc
      301 gccttctcga tcccgcgctt cagggccatg ggattgtggc ctgcggccac gagctttgag
      361 ccctcccggt agatggcctg ggccaggatg gtcgcggtgg tggtcccgtc tcccgccaca
      421 tcggaggtct tgctcgccac ctccttgacc atctgcgctc ccatgttctc gaacttgtcg
      481 gcgagctcga tttccttggc taccgtaacc ccatccttgg tgacaagagg tgcgccaaag
      541 ctcttgtcga tgaccacatt gcggcctttg ggtcccaggg taaccttcac cgcatccgcc
      601 aagatgctga cgccgttcat gattctgtcc ctggcttcgg catcgtactt gatttctttt
      661 gctgccatag ggtctttcct ccaaaagatc tcatgcgttc cctgatttca gttcgtggtt
      721 cctactgcac aatggccagg acatcgtcct cgcgcataat gaggagatct tcgccctcga
      781 tcttgatttc gtttcccgcg tacttgctga aaaggatgcg gtccccctct tggacggtca
      841 agggcgtcct ctttccgtct tccgaatact tcccgtctcc tacggccacc acttttccct
      901 gctggggctt ttcctttgcg gtatcgggaa tgatgatccc tcccgccgtc ttctcctcct
      961 cctccatgcg cttgacaacg atccgatcgg acaaaggctt gagcttcata ccaacactct
     1021 cctttgggca ttgggtcatg ccgccgtgct tgatttgggt tttgattggg tcgagatttt
     1081 cgaccgagaa agattggcac tctttgcgag tgagtgctaa cagctatttt tctaatccga
     1141 ggatcgaggt tgtcaagggg ggccccccaa ggctctcgaa aactcggctc ggggcgattt
     1201 ctgcgaagac cttgaaggat gaagcggttt gtgagaagga gaaggatgcg gatttttcct
     1261 cgggcctgga cccacggcag acctttgccc cgggtccctt catccgccaa agaccggggc
     1321 ggaaacggga acacccggtc gattcgagaa acaagacgaa tgtgccggga aaaacagccc
     1381 gataccggac tgcccgggtc tgaggggccc accggaacca ggagggaaaa agaatgcaac
     1441 acggttcttc gaacacaatc cgccaggcac ggcctacgag gcgggaattc gcggttttca
     1501 ccctgctgac cttcctcatg atcggttctt ccgcttgggc ggtccggagc ctgccgtctt
     1561 cgggggtggt cacggccgtg tttgacggag acacgatcct cctggacacc cgggatcgag
     1621 ttcgttacct gggaatcgat gcgccggaag tctcccacgg caaggaagca tccgactgca
     1681 tgggggagga ggccgcaaaa ttccactcac aaatggtcct tcacaagagg gttttcctcc
     1741 attacgaccg ggaacgccgg gacgatcacg gccgtttgct ggcctacgtt catcttcccg
     1801 acggcaggtg cgccaacgtc gagctgcttc ggggagggta tgctttcgtg cgcagaagcc
     1861 gggagggctt cgcccgcctc aacgagttcc ttggaattca gcgggaagcc gtccaggctc
     1921 gaaggggctg gtggagcaag tgtcccggga ctcccgcggg ggaatacctt ggaaatcgcc
     1981 gctcgttcgt gctacacgtc ccccattgcc cttggggaaa gcggaccgag gctcgaaatc
     2041 gagagaggtt tccctcccgg tggcgggcgt ttgaggaggg atatcgaccg tgccgagagt
     2101 gcaagccgta gacacagaga ttttgctggg ggtgagatga atcgacgata tccggatcgt
     2161 cccatggtgg gggtgggggc catcatcttt cgaggacggg aggtcttgct ggtccagagg
     2221 ggccgggaac cctcctatgg gaagtggagc atcccggggg gcttggtgga gctgggagaa
     2281 aacctccgag acgccgtaga acgcgaggtc cgggaggagt ccggcctgga ggtggaggtc
     2341 gaggaccttg ccgtgaccct ggaccgggtg attttggatg agaaccggag gatcgagtat
     2401 cattatgtgc tcctggactt cctttgccgg tggaaaggag gagagccccg agcggctacg
     2461 gatgtgctcc gatgtgagtt cgtgtccctg gatgccttgt ccggctattc gttgacccag
     2521 gggacggagg aggccgttcg gcgggtgtat cggctgagcc tgggcgaacg gcagaagatt
     2581 tacgacccgg atttgtaggc gccgggggaa agttacccgg accttttggg gggcgtttcg
     2641 gggaggggag atctttcttg actttcccgg atgcctgtgg tttatgactg accagtccgt
     2701 cactggggga atcggaggcg tttggaaagg aaaccaacca caccatgcaa cacttgggac
     2761 gcatcgtctg gaagaaggtc gcacagggaa ccgacgatcc cgaagccatc ggctgccttc
     2821 tggaacggca catctccctg gaccagcccc gaaaggttct tctgaacgag ctcgtctccg
     2881 ccttcaccga actgggccaa tccctcaaac acgacccccc cgccctccct ccttctctcc
     2941 caaggatcat tcaagggttt caacaatttg cccggaaagc ggagatcccc cccgatgagc
     3001 tctgttcctc tctgatcgaa ggaatgatcc tccatgccca tcacgaagac ggaacgcgaa
     3061 ccgcccccaa gccgtccacc caggaaaaga tcctggatgc ggccctggaa gtattttccc
     3121 acaagggatt tcacgtggcc accgtggacg agatcgccga gcgcgccggc gtgggaaagg
     3181 gcaccttgta ccgctacttc tccaacaagg agaccctgtt caacgaactg gtgcggctgc
     3241 gcctggaaga gctcgagcgg gatgccaatg cagccctcaa cgaccgcgat aacgtgctca
     3301 ccatgatcgc caaatatctg cgggtctatt tcgaattctt cgaccggaat cagcgcctct
     3361 accgggtcat tgtccaggag cggctggatg tggggaaaca ggttcaggac ctgtacgtaa
     3421 agaaggtgat gcggcgtttg cccgccctga agcggaaggt gtacgaagcc agtcagcaag
     3481 gcgttttcaa ggacgtggac tttcaaacgg tgttctacgg ggtgatggga ttcagccacg
     3541 gcgtcatcca gaagtggctg gcccgggaat gttcctactc cctggtggag gaactcccca
     3601 ccgttttgga agtccttttc tacggatttg tgaaggattc ccagtctctc ccgaaaagag
     3661 tgccctaagg aggaaatcaa tggacgtgaa tgcaatgcgg aacaaggtgg tggatattat
     3721 cgcgaatcag ctgggcgtgg acaaagaaat cgtcaccccc gaggccaacg tggtggatga
     3781 tttgggggcg gattccctgg acgtagtgga actggtcatg gccctcgagg aggcgttcga
     3841 cctggagatt cccgacgaag aagccgaaaa gatccgcacg gtcaaagaca ttttcgaata
     3901 cctggaaaag agccttcagg cctagtcccc gtactcaacc aagcccagct cccccatcct
     3961 ttccttgttc cggcgtgaga ggatggggga tcttcttccg ccccccttcc gcccttccgc
     4021 gccgggtctt cccggcccta ccgcccccag gaatccggcc cggctattct tccccggtct
     4081 cgcccggttt ggaaagctcc gaagaccaat cgggggcctg ggcattcaca acggtatcct
     4141 tcacggtcca aaagacgctt cggaaatgag catcgaagtt gccgggggac accctcccca
     4201 gctcgatgaa cttgacgacg atctccttgg caatgcgcag aaccttctcg tcatccggat
     4261 tcatggatct ccccttgcgg tttcgacata aaaaagcctc tgctgtttgg agggaaggcc
     4321 ttttgggggg tactagcaga ggcttctttt ttgggcgagc gaaatacccc ggaaggcatc
     4381 tcagggccca ccctctccat agcacaggag gggtccggaa tgcaacggga aataatgcgc
     4441 ggtttcgagg acttaagggg cggggctccc aaggggaaaa aagcggcccg gtgggcaaac
     4501 cgggtcagtt tgggtttgac aacccccaat gagcggtgtt taatgggccg tcagtggcgg
     4561 aaagtggagg aaagggggcg gaagtggtcg tcttggagaa atcctatttc aggggtcaat
     4621 ccgtagctcg cctggatgcc aaaggacggc tgcgaatccc tgccaagttc cgcgaggtcc
     4681 tgcagcaccg ccagtcggat tcggtcatca tcacctggct ggataagtgc ctggtggtgt
     4741 atcccatcga ttactggcag caaatcgaag cgaaagtcct cggtctctcc cagattcaac
     4801 cacaacatcg agcgttcgtg cgccaattca tctcgggagc tgaggaatgc ccgtttgacg
     4861 gtcaagggag agtcctgatc ccgcccctcc ttcgggagaa ggcccagctc ggccaagagg
     4921 ttcttctggc cggattgctc accaacttcg agatctggaa caagggtgcc tgggatcaac
     4981 agaacgactg gggcagggag catttccggg aaattgccct agacattgca cacaccggct
     5041 tctgatccga catggaaacg aagtcgcccg tcggtcacgt ccccgtcctt ggtaaggcgg
     5101 ctgttgatct gctcggatgt tcccccggca agctctacgt ggacggaacc gtgggcggcg
     5161 ggggatatac cgaggcaatc ctccagagat cggcccccga cgggagggtc ttgggcctgg
     5221 actgggacga ggaagctgtc gagcgggttc gggagcgttt ttcccacgag ccccgcctgg
     5281 tgctgcgaaa ggccggtttt tcggacctcc atgcgatctt ggaagaaatg cattgggggc
     5341 ccgtgcacgg aatcgtcatc gatttgggcg tgtccacgtt tcaactggat gatccccggc
     5401 ggggattcag tttccttcgg caaggacctc tggacatgcg catggatctc gggatttccg
     5461 aaaccgcggc cgacctggtc aattcccttt cccaagagga cttggcgaac ctgattttcc
     5521 gattggggga agagaggtac gcccgtccca ttgcccgggc catcgtgacc cgccgacggc
     5581 gaagaccttt cacggggacc ctggatttgg ccgccgtcat cgaaacggtg gtcccccgga
     5641 cgagggatac cctgaggatt cacccggcca cccgaacgtt tcaggccttg cggatggcgg
     5701 tgaatcagga attggaatcg ctggaagcct ttctatcccg ggtcttgggg gacttgaggt
     5761 cgggggggag gctttgcgtc gtttcgtttc attctctgga agaccggatg gtgaaggacc
     5821 ggtttcgatc ttgggcccga tcctgccggt gtcccgagga cctgcccgtc tgcagatgcg
     5881 aaggcaggcc cctggggaag cttctcacga ggaaggccgt ccgaccggac cccgaggagg
     5941 tgaaacgcaa ccccagggcc cgcagcgccc ggcttcgggc ggtggaaaag atctaggggg
     6001 agggagtcat gcataccctg aagctgagtc ccgaactgct gagccgggag aaagcggatt
     6061 ccggcacggg gctgtggaag tggctggtgg tcgtcgggac cctggcggcg gtgtttgcct
     6121 gcggggccgt attctacgtt tggctcacta ttgaacgggt gcaaaacggg taccgactgg
     6181 caaggctcca tgacgaatac gaacagctgc tctcggttca aaggaaactg cgcctggaat
     6241 gggtgcgtct ccaggacccc ttccacatgg aagaggtcgg ccggatacag ctggggctgg
     6301 ttccaccccg gcccgatcaa aagatcagcc tgcgttgacg cgcccaacgc agaaagaggt
     6361 cccggaaacg agtttgccgc aattggttac ggccgcgatg ttcgcggcac gggagtccgc
     6421 agggagtggt gaggaatgga tgcacgaaac ggcaggaatt tcctgagcgc tcgatactgg
     6481 tcctgctacg cgttctgcaa cgtcctggtc ctttccgtgc tttgcctcat tcttttcaag
     6541 gccctgcgcc tccaggtgtg ggaacatcag gtctggctcc agagggaacg ggcacagatg
     6601 gagacgacct ttcaggttcc cacataccgg ggttccatct acgaccgcca gggaaggctc
     6661 ctggcctact cggtccccca gcactccctc tacgccgact gcgaaatggt ggaaaactcc
     6721 gcacaactgg cgacccgtct ggctcccatc ctgggagaac ccgaggcgac tttggagaaa
     6781 agactggcca gcggccgcaa gttcgtctgg ctcaagcgtc acctcacgga ccagcaggcc
     6841 ctggccgtcg agaagctgcg cgcccggggg ctcaacctca tccaagagta tcgccgattc
     6901 tatccccacc ggcagattgc cggtcatgtg gtcgggtttg tgggacggga cgggacgggg
     6961 ctcgagggta ttgagaaatc tttcgaccgg gttttgcggc agagcacgac caccttttcc
     7021 caacacaggg acggggttcg caagtacctg tggctcaatg cggacccgcc gcccgaaatc
     7081 aaggaaaact tcggggtgac gctcaccatc gacgcatacc tccaatacct gtgcgaaagc
     7141 gaactcgaga agtgcgtcca aaagtaccgg gcggcctcgg gtgaggtggt cgtgatggat
     7201 cccgacacct tcgaaatcct ggccatggcc aactggcctt ctttcgatcc caatctcgag
     7261 ggaaagaaga acgcggaaat ctggagaaac cgatccatta ccgatttctt cgaaccgggt
     7321 tccaccttca aggtgttcct ggtgagcgcg gccctggagg aggggagcgt ccgggaaaag
     7381 gaccgggttt tttgcgaaaa cggcaagtgc gtcctggcgg gccacatggt caacgacgtc
     7441 catccttacg gatggctcac catccccgag gtgatcaagt attcgagcaa cattgccgcg
     7501 gggaagttgg ccctccagtt gggcggagag cggtatcacc gacacatcaa ggggttcggt
     7561 ttcggttccc gcacgggggt cgagctcccc ggagaagccc ggggattggt tcgcagctgg
     7621 aagaagtggc gccccatcga ccttgccacc acggggttcg gccagagcat cggcgtcacg
     7681 gccctgcagc tcacggccgg gatcggttgc atcgccaacg ggggagaatg gaagcagagc
     7741 cgggtggtcc taggaatcgt ggacagcgat tcgaggccga cggagcccct gaggaccaaa
     7801 cccacccgcc ggacaattca atccaagacg gcgcagcaga tccgtgatat gatgcaaagt
     7861 gtgactcaag aaggcggtac cggagtgaat gcggctcctg aaggatattc cgcggcaggc
     7921 aagaccggca cggcacagat gctggaccct gccacgaaac ggtatgcttc caacaaatac
     7981 acgtccctgt ttaccggttt tgtccctgca gagaagcctc gcctggtcat cagcgtcgtc
     8041 atccacgaac cccacggggc catctacggt ggagtggtgg ctgcccccgt cttcaagaac
     8101 gtggcggcaa aagcgctccc gtacctgggg gtgtcgcctt cccccgggag aaccggcaac
     8161 ggtccgaaac tgaagctggt gaatgcaccg aaaaaggaat cgaccaagtc tcctcctgcc
     8221 gtcgagaacg cggcatcgag caagatgccc gacgtttcgg gattgagtct tcaagaggcc
     8281 cttcaacgac tggcccccct ggacgcccag gtgaagctgc ggggccgagg gaccgtggtg
     8341 agccagtctc ccgaggtggg tacgccgttg aagtccaaag gaaccgtgga attggtgctg
     8401 aacgacgtcc gggaaaccgg gactcgatga ggttgcttaa ggggtcatga atccgggggg
     8461 cgcgggagcc ccccaaagga acggacggca aatgcagaag gcactgggta ctttgttgga
     8521 tggcctggaa gtcctcgacg cccggggaaa catcggcact cccatcggcg gggtggccta
     8581 cgacagccgg caggtctcac ggggagacct gtttgtggcg gtccgaggaa cccggcagga
     8641 cggcggggcg ttcatccggg acgccgtcca acggggagcc caggccatag tggccgaatc
     8701 cgaaccggaa aacggggtcg ctctgccctg gatccgggtc cccgacagtc gcagggccct
     8761 ggctcgtctt tcggcaaact tcttcgacca tccctcccgg ggattgaacc tcatcggggt
     8821 gacggggacc aacggaaaga caaccaccac cctgctcctg gaagggatcc tggcccaggg
     8881 gggccatggc gtcggggtcc tgggcaccct gggatatcga tggcccggaa cccggcgggc
     8941 cgcacccatg accactcccg aatccctcga cctgcagcgt ctcctccacg agatgcgggg
     9001 agccggggtc acccacgtgg tcatggaagt ctcctctcat tccctggcct tgggccgggt
     9061 ggaaggctgt tttttcaagg cgggcgtttt caccaacctg agccgggacc acctggattt
     9121 tcacgggagt atggaagagt actttgcggc caagtccctg cttttcactc aattcctatc
     9181 ttcgggccgg gaacgcggag catcggtcat caaccttgac gatccatacg gcgaacgact
     9241 ggcccggtcg gtggcggggg aaatctggac ctactcggtg gatcgccccg aggcgacggt
     9301 ccgagtccga ggcgcggacc tggggacccg gggaatccgg gcggaactga tcaccccggc
     9361 gggactcctg aagatcgagt ccccgctgct gggaaggctc aacctgtaca atctcctgtc
     9421 ggcggcttcc gcggcccttt ccgtgggaat tcccggggag gccgtcagga gcggtctggc
     9481 cgaaattcat tccgtggacg ggcgtctcca gcaggtggcc gttccctcgc gcttggactt
     9541 ccaggtggtg gtggactacg cccatacccc cgacgccatg gaaaagagcc tgagttgtct
     9601 ccgggaaatg actcgaggac ggctggtggc ggtctttgga tgcggcgggg accgggaccg
     9661 gggcaagcgc cccctcatgg gaaagactgc cgcccgttgg gccgacctgg tggtcatcac
     9721 cagcgacaat ccccggacgg aggttcccga ggagattatc cgggaaatcg agccgggggt
     9781 gcgggaagaa ggcatgccgt acctcgatcc cgaccgggag gatcttcccg cccgggggta
     9841 ctcggtggtc gcggaccgca ggaagggcat cgagctggcc ctgtcctggg ctcgtgcggg
     9901 agatgtgatc ttcatcgggg ggaaagggca cgaaacctat cagattgtgg ggaccaccgt
     9961 ccttcccttc gatgatcgcg tggtggtccg ggagtttctg ggcagcgggg aagaatcctc
    10021 gtagccgcgc agggtgagca tgaccatgaa ctggacgaga tcggacatcc tggaagccac
    10081 ccggggcacc cttctgcagg gttcccccgt cggctccgtc ggcaggattt ccaccgacac
    10141 ccgcagcatc gaaccggggg acgccttcgt tgccctgcgg ggggaaaacc acgatgccca
    10201 cgatttccta ggggcggcgg tggaaaaggg agccgaaacc ctcgtggttt cccgactccc
    10261 ggacgaacgg cctgttccgg cgccctccca tgtgaccgtc gttcgagtgc cggacacctt
    10321 gacggccctg ggacagctcg ctcgattcca ccggtcccgg ttcacggttc ccgtggcggg
    10381 gatcaccggc agcaacggaa aaaccagcac caaggagatg gtgtccgcca ttttggggca
    10441 gggcaggaag gtgctcaaga accgcgggaa cttcaacaac ctggtgggag ttcccctcac
    10501 gctcctgtct ctcgagccgg accacgagat tgccgtggtg gagatgggga tcaatgttcc
    10561 cggggagatg gatcggctgg tgcacatggc cgtgccgacg gccggcatcg tcaccaacgt
    10621 tcatccggct cacctcgagg gactggggtc cctggatcga atcctcgaag aaaagggaaa
    10681 gctgctctcg agcctgggag ccgggggcgt ggccgtcatc aacctggacg acgagcggct
    10741 ggaaacattc tcgaaaaggc tcgagtgcag gcgggtgacc tattccctgc aaaagcagga
    10801 agccatggtg gctctcgggg gtcccgtgga ggtgaaggaa ggggtcagtg tgtttcccct
    10861 gcgtctggga aaggagaccg tccccgcccg gctttcggtt ttggggatgc accaggttca
    10921 aaacgccgtg gccgccgcag cccttgcctg ggcattgggg gaatcccccg agagcgttgt
    10981 ccgtggtctt tccctgcatc agccggtgaa gcagcgtatg cagatgcacc ggcttgccgg
    11041 agaaagaatc ctcatcgacg atacgtacaa cgccaatccc cagtccatgc tcgcttcggt
    11101 gcggaccgtg gtggccgcaa gctcggggag acccgtcctg gcggttctcg gggagatgag
    11161 ggaactgggg ccggggagcg ccgctcttca ttatgaagtg gggttccagg tcggggcctc
    11221 gggaattcgc ttcttgatca ccatgggaga attcgggcgg gagatccaac gaggtgctcg
    11281 ggatgccggg ctttccgctt cgtcgtgctt ccatgcggga agccatgagg aagcggtgga
    11341 gcggctccgg gattggatgc ctcgggatgc ctggatcgtc gtaaaggggt cccgaggtat
    11401 gaccatggag cgggtcgtgg aagggatgat ggactcatga aagcgatgcg gccgggtgcc
    11461 gccatgccca agaaggccct ggtggtgggg ctgggaatat cgggaagatc ggtctgtgag
    11521 ctgctttttc gtcacggtgt ccgggtgacc gccacggacc tgaaaaggcg ggaagaattc
    11581 ggaggaatgc tggattccct cgaagcaaag ggctgcgggc ttcgcctggg gacccaccgg
    11641 gtggaggact tcctggaggc cgaccagatt atcgtgagtc ccgggattcc cctcgagatc
    11701 gaacccctgc gaaaagcggc ccaaaagggg atcgagatcg tgggagagct cgagtgggca
    11761 tggcgcatga ccacgacccc ggtcatcgcc gtgaccggca ccaacgggaa gaccaccacc
    11821 acctccctca tcggcgaaat gctgcggcgg gcgggaaagc aggtcttcgt ggggggaaac
    11881 atcgggactc ccttgagcga gtgggttgct tccggtcggg aagcggattt cctcgtgttg
    11941 gaggtcagca gctttcagtt ggacacggct cctcgattct gcccggagat cggcatcctg
    12001 ctaaacgtca ccgaagacca tctggatcga tacgacggct tctcttccta cgcggactcg
    12061 aagctgtccc tcttcaaaag gcaaacctcg tcccacttgg cgattctcaa tggggacgat
    12121 cccgtttgca cggcgcgcgc cggggatctc ccaggccggg tgctgtttta cagccgcaac
    12181 gattccacag cccatgccgt tctccgaaat cgggaagtgc gggtgcggct gccgggagga
    12241 gaagactact tgttctctct caaggggtcg tctcttcgcg gagtgcacaa cgaggagaac
    12301 atcctggctg cgggccttgt cgcggcgtcc ctggggatcc cccgggaagt cctggaacag
    12361 acggtccggc aataccgggt tttgccccat cggctgcaat gggtgcgcac ctggatgggg
    12421 atcgatttct acgacgattc caagggaacc aacgtggggg cggtggtcaa ggccctcgag
    12481 aacttccgac agccggtgct gctcctcctg ggaggaaagg acaagctggg gtcctactcc
    12541 cccatcggag aacggatgaa gaggctggga aaagccgtct atgcctttgg agaggcggct
    12601 ccccgcatgg tccaggagct cgatccctgg gtttccgtcc gatcctttgc gaacctgccc
    12661 gaagcctttc gggaggccgt aaaagacgca cgatcgggag acgtgg
//
LOCUS       DAOOVC010000110        12540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3315_length_12540_cov_5.856468, whole genome shotgun sequence.
ACCESSION   DAOOVC010000110 DAOOVC010000000
VERSION     DAOOVC010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3315_length_12540_cov_5.856468"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLM79_12940"
     CDS             <1..445
                     /locus_tag="PLM79_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOV87264.1"
                     /translation="RPDLLAEAHGPAVQKGSSMKPVLVGFLLVSALTAGTLSWFASSN
                     PDGLEWSISKTTGKEELEAPGDTVHAFLAKIQEKIAFLPGYDFKDTASSQHPSEGSTE
                     SREEPWPAVNAGTTVAGLVGGAMTLLAVSVIAFGLRRLQARPNER"
     gene            474..935
                     /locus_tag="PLM79_12945"
     CDS             474..935
                     /locus_tag="PLM79_12945"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOV87265.1"
                     /translation="MNVFEEFKQLVLELEKQTVRYALVGGVAMAFYAEPRFTRDIDLL
                     VDPEDFDKIKGIIEKYGYLESASPWTFPNVAIEFHRFLKVVDEEDEMLIDILVAGNEE
                     VGNIIRNAVEAESEEGKVMIAHKRDLIWLKRTRDSKQDQADIEKLEDDKDR"
     gene            919..1056
                     /locus_tag="PLM79_12950"
     CDS             919..1056
                     /locus_tag="PLM79_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87266.1"
                     /translation="MTKIDRVLKKLSELYHFNRKLKAYKLKEKANKRLEPATRPRGDN
                     Q"
     gene            1349..2161
                     /gene="cbiQ"
                     /locus_tag="PLM79_12955"
     CDS             1349..2161
                     /gene="cbiQ"
                     /locus_tag="PLM79_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOV87267.1"
                     /translation="MARIETSVFDLSQLDLLSCGQTVVHRLDPRVKVLTTLFFTGIVV
                     SFPKYEIAPLLPFALYPAVLISLGNLPPGHLMKKVLWAAPLAVLIGIFNPLLDTAILV
                     QLGPFSLSGGWISFGAILVKFLLTVTTALCLVATTGFNAVCTALDRMGVPRGFVIQLQ
                     FLYRYIHVLSDDAARMSRARSLRSFNGRGMGMQVFASLVGQLLLRTLDRARRIHAAML
                     CRGFDGEIRLLRPLNIRATDIGFLAGWCGLFVFLRLYNLPRWLGRLAAGTAL"
     gene            2158..2910
                     /locus_tag="PLM79_12960"
     CDS             2158..2910
                     /locus_tag="PLM79_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87268.1"
                     /translation="MSHHIVEVKDLHFAYPDGHKALEGVSLRITHGERVGVVGANGAG
                     KSTLLLHLNGTLIPQSGTVRVGDFPLTRKTLPHVRRTVGMVFQDPDDQLFMPTVYDDV
                     AFGPLNLGFPHEEVHDRVMKALETVGADHLRDRPPYRLSGGEKRAVAIATVLSMAPDI
                     LVMDEPTSNLDPRSRRQLIRLLDTFEHTLIVATHDLDMVLDLCPRTVVLKDGHVAADG
                     PAREMLRDEPLLHACNLEKPLVLQACPVCTRV"
     gene            complement(2951..3871)
                     /gene="era"
                     /locus_tag="PLM79_12965"
     CDS             complement(2951..3871)
                     /gene="era"
                     /locus_tag="PLM79_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOV87269.1"
                     /translation="MTKSSEQRPFKSGYVALVGAPNVGKSTLLNGIMGEKISITAPKP
                     QTTRNRILGILTRPDCQAVFVDTPGIHKARDPFNKILVDTALSTLNEVDVVCFLLEVP
                     ETHKHLDSFILENLRRIRSPVILVVNKVDRLPHKPDLLPLMDHYRNLFPFHAIVPTSA
                     LKGDGVEELLGEILGLLPEGPRYYPEDYSTDQPERFLAAELIREKIFHLLRQEVPYAV
                     AITVDQFKEEPDRNLIDIEATIHVERDSQKSILIGRQGRMIKEIGKRARGDIEILLGC
                     RVFLGLFVRVTRDWRKNPRSLREFGYLQNR"
     gene            complement(3868..4950)
                     /locus_tag="PLM79_12970"
     CDS             complement(3868..4950)
                     /locus_tag="PLM79_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87270.1"
                     /translation="MNRNRIYPVFLPHLGCTFRCVYCNQNVTSGTAPFSRDPSRVLPR
                     ILEDIEELSRSAVACRKPGQIAFYGGTFTALPTEILEEVLDAAAFHVRRGTFTGIRFS
                     TRPDAVTRTVCSLLKSFPVQTVELGVQSLSDEVLRESRRGYGADAVLDALQRVRSSRW
                     DVGFQLMPGLPGDSPDRFLGTVTDTLAMKPDFVRLYPVLVLPGTTLAGWYEAGRYRPL
                     SFEEAIRWCARAYDRFHGAGIRVVRMGLHAGPELTNPGSVLAGPFHPSFGYLVRVRWW
                     RNRIDEAIRTRPAFGKGARLILWVGERFVSEVLGPDRSNVSYWLECRGLENVQVQGDK
                     NLHRGEYRIVLESESMPRPQGERSFS"
     gene            complement(5009..5539)
                     /locus_tag="PLM79_12975"
     CDS             complement(5009..5539)
                     /locus_tag="PLM79_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87271.1"
                     /translation="MERTVETKTGERIRLRVERDAHQVPQVYAVVGDGEGGEERRLLC
                     HGWVVRRLPEGICGGIHAQGVFLEVDRKDWDAVLAEREDLRDRDNLPDIHLVRVFSRG
                     DRFTIDGYSLSARVDRATWNRIKVHMLEVDSFENGEVLEGDHFRGWIIRQGMESEVER
                     LLDVKAHLRLRSRQGG"
     gene            6171..6698
                     /locus_tag="PLM79_12980"
     CDS             6171..6698
                     /locus_tag="PLM79_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:6570..6572,aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOV87272.1"
                     /translation="MRPEKDDKGWNPYLAGALTGLLLVASTWVTGKYVGASTSFARSA
                     GMIEQLFAPERVKIMEYFVKEKPIVEWQWMFVAGIALGAFLSAVTSGTFRWKALPDMW
                     EERFGPSRGKRAAVAFLGGAVALFGARLADGUPSGHGLSSSLQLAVSGFIALVGFFAG
                     GIPVARLLYGGGKQS"
     gene            6695..7222
                     /locus_tag="PLM79_12985"
     CDS             6695..7222
                     /locus_tag="PLM79_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOV87273.1"
                     /translation="MMTLIYGLVTGVIFGFLLQKGRVLRYDKQLGALRFLDMTIVKFM
                     FTTILVSMVGVYLLKDLGLVKLAPKATMVGANALGGIVFGIGWGLLGYCPGTSLGAVG
                     EGRWDAIWGIAGGLVGAALFAETYPFMKNTVLKWGNYGPITLPQVLGINHWILIAVFV
                     AGGIALFAWFEKKGL"
     gene            complement(7228..7986)
                     /gene="rnc"
                     /locus_tag="PLM79_12990"
     CDS             complement(7228..7986)
                     /gene="rnc"
                     /locus_tag="PLM79_12990"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOV87274.1"
                     /translation="MSQSSVLEELEQALHYRFRKGNLLFQSLVHRSYVHENPHLRQAD
                     NETLEFLGDAVLGLAVSHLLLDRFPQCREGELSRFRSSIVNEKELAKVATDLDLGRCL
                     LLGKGEELTGGRNKPSLLADTLEAVLAAVYLDGGMDEAIRVVSVLFHEYFNAGDANLS
                     LRTLDKDYKTQLQEITQARHRMTPVYQLKAEEGPDHNKTFSMSVVLGERVLAHGSGKS
                     KKEAQQEAARRALDLLQSESDFPGAGSESPGVQE"
     gene            8088..8675
                     /locus_tag="PLM79_12995"
     CDS             8088..8675
                     /locus_tag="PLM79_12995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV87275.1"
                     /translation="MLVWIQHPRPSSRRFLFLDRDGVINVDSSKYIKNWDEVRFYPDA
                     LDALRFLRQQSVQGILISNQSVVNRGFTSIETLWDIHDRMLDRIRRAGGDVLAAFYCP
                     HRPDEACACRKPSPRMILYAAGIFSIETAETYLIGDRSSDILAGGDSGCRTVLLERPD
                     SEHPPEGAWAGRQPDERYPSLLEAVTALWKQGKLS"
     gene            8740..9411
                     /locus_tag="PLM79_13000"
     CDS             8740..9411
                     /locus_tag="PLM79_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HOV87276.1"
                     /translation="MFYPPFLLFFLFAFLFLLLFAFALVQFGIFSWAFTKLGISPEHL
                     FSLLFLSIVGSMINIPIRKIPIETDMQDPDIVSFFGMRFRPPRRQRVREMVLAVNVGG
                     ALIPTGLSVYLFFHSPHPFRMLLALAAVTCVVYRAAKPVPGLGIATPMFIPPLVAALA
                     ALLLDPRSAPATAYTAGTLGTLIGADLLHLDKLKELRAPVASIGGAGTFDGIFLTGIL
                     AVLLA"
     gene            9448..10578
                     /locus_tag="PLM79_13005"
     CDS             9448..10578
                     /locus_tag="PLM79_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV87277.1"
                     /translation="MASKVFFIDLAVRNNQGLLDRIPSLLEAAGIAEVVPDRALTAIK
                     IHFGERGNTAFVRPLLVRPIVDAVAGAGGRPFLTDAGTLYVGTRGNAVDHLNTAMLNG
                     FAYSVVGAPLVIADGLDGGDEVAVPVELKHCRVAYVASAVAKARALVSVAHFKLHEAA
                     GFGGAIKNLGMGSASRKGKMAQHSDSGPLLEASKCIGCGLCREQCAHDAITLVDRADA
                     ESSLPMASIDHGLCVGCARCIHTCPQGALGINWEKDLPRFMERMVEYTAATLKGKEEN
                     LLCINFLTQVSPACDCYPFADSPVVADIGVVASRDPVAVDQASMDLVNAQPPLGSSCI
                     KDSPHASHDKVRAVYPQVQWEHQLAYAEQLKMGSRTYDLVKL"
     gene            10712..10810
                     /locus_tag="PLM79_13010"
     tRNA            10712..10810
                     /locus_tag="PLM79_13010"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10747..10749,aa:Sec,seq:tca)
     gene            complement(11024..12232)
                     /locus_tag="PLM79_13015"
     CDS             complement(11024..12232)
                     /locus_tag="PLM79_13015"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HOV87278.1"
                     /translation="MDELRGFHEQFRDCFTRSEPREHFRLYMSGQFSPLERKSIEPIA
                     LQIEDGKVRAMQRFVSDVVWDEPLMLRRYHTLVAEDMGDPEGVLILDESGFAKKGDDS
                     IGVARQYCGSTGKVDNCQVGVFAAYASRHGYAFLDKRLYLPRNWFEETHSERRRKCRM
                     PVELTFQTKPQLAAEMYRDLRAEGIVPFKYVVADSVYGANPDFIGAVEGRVGTTYFVS
                     MPSDTLCWLQGPSTEQRAVRGKGGTKVRRAVSDGEKKPVDFATLAGLIHSFFWYRRKV
                     SEGTKGPIEYEFTKRRVTLCKGGLPDKTVWLVIKRTLGTNREYSYYISNAPVSARLEL
                     FVWLSGVRWAIEQCFEEAKSELGMDHYEVRKYPGWNHHMLTCMLAHFFLRHLSIKSGK
                     KSTVHYTVAA"
BASE COUNT         2419 a         3819 c         3683 g         2619 t
ORIGIN      
        1 tcgacccgac ctgctggcgg aggctcacgg cccggccgtg caaaagggtt cgtccatgaa
       61 gcccgtgctt gtcgggttcc tcctggtgtc cgccctcacc gccgggaccc tctcctggtt
      121 tgcttcctcc aatcccgacg ggctggaatg gtccatctcc aagacaacgg gcaaggagga
      181 actcgaggcc cccggggaca cggtccatgc tttcctggca aagatccagg agaagatcgc
      241 ctttctgccc ggctacgact tcaaggacac cgcgtcctcg caacatccct cggaaggctc
      301 aacggaatcc cgggaagagc cctggcccgc cgtcaatgcc gggactaccg tggccggttt
      361 ggttggagga gccatgacgc tgttggcggt gtccgtgatc gccttcggct tgaggaggtt
      421 gcaggcccgg cccaacgagc gttaggggtt tggtcgccca ttaggaagaa agcatgaatg
      481 tatttgaaga attcaaacag cttgtgttgg agttggaaaa gcagacagtg aggtatgccc
      541 tggtaggtgg ggttgctatg gccttctacg ccgagccgcg atttacgagg gacatcgatc
      601 tcctcgttga ccccgaagac ttcgacaaga taaaaggcat tatcgagaag tatggatatc
      661 tcgaatccgc gtcgccatgg acattcccga acgtagccat cgagtttcac cggttcttga
      721 aagtcgtaga cgaagaggat gaaatgctga tcgatattct cgtcgccggg aatgaggagg
      781 tcggaaacat aatccggaat gcggtggaag cagagtcgga agagggaaaa gtgatgatcg
      841 cgcacaaacg tgatctgatc tggcttaaga ggacgaggga ctccaagcag gatcaggcgg
      901 atatagaaaa gcttgaagat gacaaagatc gatagggttc tcaaaaagct cagtgagctt
      961 taccatttca atcgaaagct caaagcctac aaattgaagg aaaaggccaa caaacggttg
     1021 gagccggcca cccgaccacg cggggacaat cagtagtatc ttttcggaca tctgggccgg
     1081 gtggtcgggt gccgctcaac cgtggaggtg cggatgtccc ggggtcgtgt cccgcagacc
     1141 gccgcaacga cgagagcccg tggccgggtc ttctcatgga ttccacacct gcccgaaccg
     1201 gggttccgta cgccggggga atccccggcc aaggcccgtt tacggatacc cccccttcgg
     1261 ggaacgctcc tcaccggtgt tccccggaaa ggacgggtcc ccaaaccctc ctctccggga
     1321 gatcttcgtt cccgggaaac gacctgtcat ggcaagaatt gagacttcgg tttttgacct
     1381 ttcccaactg gatcttttgt cctgcgggca gaccgtcgta catcgcctgg atccgcgggt
     1441 gaaggtgctc accacgctgt tctttacggg gatcgtggtg tcgtttccaa aatacgagat
     1501 cgctcccctc ctcccctttg ccctttatcc cgccgtcctg atttcccttg gaaacctgcc
     1561 cccgggacat ctgatgaaga aggtcctctg ggccgctcca ttggctgtgc tgatcggcat
     1621 cttcaacccg ctcctggata ccgcaatcct cgtgcaactg ggtccctttt ccctttccgg
     1681 cggatggatt tcctttgggg ccatcctggt gaaattcctc ctgaccgtga ccacggccct
     1741 ttgcctggtg gccacaacgg gcttcaacgc cgtttgcacc gccctggacc ggatgggggt
     1801 tccccggggc tttgtgatcc agttgcagtt cctttatcga tacatccatg ttctcagcga
     1861 cgacgcggcc cgcatgagtc gggctcggtc cttgcggtcc ttcaacggca ggggcatggg
     1921 catgcaggta tttgcatccc tggtgggaca actgctcctg cgcaccctgg accgggccag
     1981 gcgaatccat gcagccatgt tgtgccgggg cttcgacggc gagatccgcc ttttgcggcc
     2041 tttgaacatc cgggcgacgg atatcggatt tcttgcgggt tggtgcggcc tctttgtttt
     2101 cctgaggctt tacaatcttc cccggtggct gggacgcctg gcggcaggaa cggccttatg
     2161 agccaccaca ttgtggaggt gaaggacctg catttcgcct atcccgacgg gcacaaggcc
     2221 ctcgagggag tctcccttcg catcacccac ggcgagcggg tgggggtcgt gggagccaac
     2281 ggggccggga aatccaccct gttgctccac ctgaacggga ctctcatccc ccaatcgggg
     2341 acggtgcggg tcggggactt tcccttaacc cggaaaactc tcccccatgt gcgcaggacc
     2401 gtgggcatgg ttttccagga cccggacgac cagctcttca tgcccacggt ttatgacgat
     2461 gtggccttcg gccctctgaa cctgggcttc ccccatgagg aggtacacga ccgggtcatg
     2521 aaggccctgg agacggtggg agccgaccat ctccgggacc ggcctcctta ccggctttcg
     2581 gggggcgaaa agcgtgccgt cgccattgcc acggttcttt ccatggcccc ggatatcctg
     2641 gtaatggacg aaccgacctc gaacctggac cccagatccc ggcggcagtt gatccgactc
     2701 ctggacacct tcgagcacac gctcatcgtc gcaacccacg acctggacat ggttctcgac
     2761 ctctgcccgc gcaccgtggt cctcaaggac gggcacgtgg cggccgacgg ccccgcccgg
     2821 gaaatgctcc gggacgaacc gctcctgcat gcctgcaacc tcgaaaagcc cctggtcctc
     2881 caggcttgcc ccgtatgcac ccgagtctag ctccccgggc cgcttcgccc cggtgccgcg
     2941 ttcctgaatc ctaccggttc tgcaaatagc cgaattcccg gaggctccgg ggatttttcc
     3001 gccagtccct ggtcacccgg acgaaaagcc ccagaaacac gcggcatccc agcagaatct
     3061 cgatgtctcc acgggcgcgc tttccgattt ccttgatcat ccggccctgc cggccgatga
     3121 ggatggactt ttgggaatcc ctctccacat gaatggttgc ctcgatgtcg atgagatttc
     3181 ggtccggctc ctccttgaac tgatccacgg tgatggccac ggcatagggg acttcctggc
     3241 ggaggaggtg aaagatcttc tcccggatca attccgccgc gagaaagcgc tcgggctggt
     3301 cggtggaata gtcttccgga tagtatcgcg ggccttcggg gagcagtccg aggatttcgc
     3361 ccaggagttc ctcgaccccg tctcctttga gggccgaagt ggggacaatg gcgtgaaacg
     3421 gaaacaggtt tcggtagtgg tccatgagag gcaacaggtc cggcttgtgg ggaagacgat
     3481 ccaccttgtt gaccacaaga atcacgggcg aacggatcct cctgaggttc tcgaggatga
     3541 aggaatccag gtgcttgtgg gtttccggaa cctcgagaag gaagcagacc acgtcgacct
     3601 cgttcagggt ggacaaggcc gtatccacga ggatcttgtt gaaggggtcc cgggccttgt
     3661 gaatgccggg cgtatccacg aaaacggcct ggcagtcggg ccgggtaagg attcccagga
     3721 ttcggtttct cgtggtctgg ggcttgggag cagtgatgga aatcttctcc cccatgatgc
     3781 cgttcaggag ggtggacttg cccacgttgg gagccccaac gagtgccaca tacccggatt
     3841 tgaaggggcg ctgttccgat gatttcgtca tgaaaaggac ctttctccct gtggacgggg
     3901 catgctctcg ctctccaaaa cgatccgata ttccccccgg tgcaggttct tgtctccctg
     3961 aacctgaacg ttctccagtc cccggcactc gagccagtag gacacattgg agcggtccgg
     4021 tcccagcact tcactcacga accgttctcc aacccacaag atcaggcggg cccccttccc
     4081 gaaggccgga cgggttcgga tggcctcatc gatccggttt cgccaccagc gaaccctcac
     4141 caggtatcca aaggacgggt gaaaggggcc ggccagtacg ctccccggat tggtcagctc
     4201 aggacccgca tggagcccca ttcgaacgac tcgaatcccg gccccatgaa accggtcgta
     4261 ggcccgggca caccaccgga tcgcttcctc gaaggacaaa ggccgataac gcccggcttc
     4321 gtaccacccg gccagggtcg ttcccggaag gaccagcacc ggatagaggc ggacaaaatc
     4381 gggcttcatg gcaagggtgt cggtgaccgt gcctagaaaa cggtccgggc tgtctcccgg
     4441 cagacccggc atcagctgaa agcccacatc ccacctcgag gaacgcaccc tttgaagggc
     4501 atcgagcacc gcatcggccc cgtagcctcg cctgctctcc cggagcacct cgtcggagag
     4561 actctggacc cccagttcca ccgtttgcac tggaaaggat ttcaggaggg aacagaccgt
     4621 tcgagtcacc gcgtccgggc gggttgagaa acggatgccg gtaaacgtcc cccggcgcac
     4681 gtgaaaggcg gctgcgtcca ggacttcttc caggatttca gtcggcagag ccgtgaaggt
     4741 tcccccgtag aaggcgattt gtcccggttt gcggcaggca acggcgctcc gggacaactc
     4801 ttcgatatcc tccaggatcc tcggaagaac ccgggacgga tcccgggaaa aaggcgcggt
     4861 cccgctggtg acgttctggt tgcagtaaac acaccgaaac gtacacccca ggtgcggcaa
     4921 aaaaaccggg taaatccgat tcctgttcac tgcgatcctt cccggggacc ggagaagtgc
     4981 ctcccgtggg tacggcaacc ctgcgggttc acccaccctg tcgcgacctc agtcgcaggt
     5041 gggccttcac gtccagcaac cgttccacct cgctttccat gccctggcgg atgatccagc
     5101 ctcgaaaatg atccccttcg aggacctccc cgttctcgaa gctgtccacc tcgagcatat
     5161 ggaccttaat cctgttccag gtagcccggt ccacccgggc gctcagggaa tagccgtcga
     5221 tggtgaaccg gtctccccgg gaaaataccc gcaccaggtg aatgtcggga agattatccc
     5281 gatcccgcag gtcttcccgt tccgccagga cggcgtccca atccttccgg tccacttcca
     5341 ggaagacccc ctgggcatgg atccccccgc aaatgccctc gggtaaacga cgcaccaccc
     5401 agccgtggca aagaagccgc cgctcttccc ccccctctcc gtcgccgacg acggcataaa
     5461 cctgggggac ttgatgggcg tcccgctcga ccctcaatcg aattcgttcc cccgttttcg
     5521 tttcaaccgt tcgttccatg aaacctcctc cggaaggcac gcccattccc aaccatgcct
     5581 gacaccgggc gcggccccaa aagtaaccct gctcctgtat catatccccg aaaactttcg
     5641 gcttgtccac cctcatatgc tcgagaggtc ctcttgtctc atttcctccc cgagggacct
     5701 tgaatctcgg ggccgaggtc cgggcggacc gaaagcgcga ccttgcgaga ctccggtcga
     5761 gccaccccgg acggcaggga ttggttccgt ttttcactat tcgatcgctc ttttctcagt
     5821 catggaaccc ttagccgaac agaccgtccg gcttccacgg atagaagtcg gcacaccggg
     5881 agggagttcc cttgggctcc cctgcaaaag acgaggggta ccccctccgg acgagccccg
     5941 gctttacaaa aaagaggggc gtgattaaaa gggtcgggaa tccgaggttc ccccacccgt
     6001 tcccggggga gttcgacgca gatcaaacaa catcgaacaa cccgaaaccc acgccgggaa
     6061 gggcttcgtt tgccataaac cccggggacc cgcagtccca aggcgccaat ccgcacccct
     6121 cgatttcttc ggaggggaag acatagaaac ctatgaagga ggacgcaacc atgagaccgg
     6181 aaaaagacga caagggatgg aacccgtacc ttgcgggcgc cctcaccggc ttgctgcttg
     6241 tagcctctac ctgggttacc ggcaagtacg tcggcgcatc cacttccttt gcccgttccg
     6301 cagggatgat cgaacagctg tttgccccgg agagggtcaa gatcatggaa tactttgtca
     6361 aggagaagcc catcgtcgag tggcaatgga tgttcgtggc gggcattgcc ctgggggcgt
     6421 tcctgtccgc cgtcacctcg gggaccttcc gatggaaggc gctgccggac atgtgggagg
     6481 aacgtttcgg tcccagccga gggaagcggg cggccgttgc cttcctgggc ggagccgtcg
     6541 ccctgttcgg agcccgcctg gcggacggct gacccagcgg acacgggctg agcagttcgc
     6601 ttcagctggc cgttagcggg ttcatcgccc tcgtgggctt ttttgcaggc ggaatccctg
     6661 tagcccgact tctttacggc ggagggaaac agtcatgatg acactgatct acggcctggt
     6721 taccggcgta atcttcggct tcctgctgca aaagggaaga gtgcttcgct acgacaagca
     6781 actgggggcc ctccgtttcc tggacatgac catcgtcaag ttcatgttca ccaccatttt
     6841 ggtgagcatg gttggggtat acctgctcaa ggacctgggc ctcgtgaaac tcgcccccaa
     6901 ggcaaccatg gtgggggcca acgccttggg aggaatcgtt ttcggcattg ggtggggcct
     6961 gttggggtac tgccccggga catctttggg ggcggtggga gaaggccggt gggatgctat
     7021 ttggggcatt gcgggcggtt tggtcggagc cgcactcttt gccgaaacct accccttcat
     7081 gaagaacacc gtgctcaaat ggggaaatta cggccccatc accctgcccc aggtgctggg
     7141 aatcaaccac tggatcctca ttgcggtctt tgtggcgggg gggatcgccc ttttcgcctg
     7201 gttcgagaaa aagggtcttt agatgcgcta ctcctgaacg cccggagact ctgatcccgc
     7261 ccccgggaaa tccgactccg attggaggag atcgagggcc ctgcgggcgg cttcctgctg
     7321 ggcttccttt ttgcttttgc ccgatccgtg ggccagcacc cgctcaccca ggaccacgct
     7381 catggagaag gtcttgttgt gatcgggtcc ttcctcggcc ttcaattgat acacgggggt
     7441 cattcgatgc cgagcctggg tgatctcctg gagctgggtc ttgtaatcct tgtccagagt
     7501 ccgaagggac aggttggcgt ctccggcatt gaaatactca tgaaacagga cactcacgac
     7561 ccggatggcc tcatccattc ccccgtccag gtagaccgcc gcaaggactg cctcgagggt
     7621 gtcggccagc agggagggct tgttccttcc tcccgtgagc tcttctcctt ttcccagcag
     7681 gagacaccgc cccaggtcca ggtccgttgc caccttggcc agttccttct cgttgacgat
     7741 ggaagaccgg aaacgggata gctccccttc ccggcactgg ggaaagcgat ccagcaggag
     7801 atggcttacg gccaggccca acacggcgtc tcccagaaat tccagggttt cgttgtctgc
     7861 ctgacgcagg tgcggatttt cgtgaacata ggaccggtgg accaaggact ggaagagtag
     7921 atttcccttg cggaaacggt agtgcagggc ttgttccagt tcctcgagga cgcttgattg
     7981 actcatttca ttttgctacc ttacaaacgt ggttatggaa cccttccaat tcttcttgga
     8041 tccgaatggg atgtcaagag cgaaaggttt tcccgcgagg agccctgatg ctggtgtgga
     8101 ttcaacatcc caggccttcc tcccgccggt tcctgttcct ggaccgggac ggggtcatca
     8161 atgtagacag cagcaagtac atcaagaatt gggacgaggt ccgcttctac ccggacgccc
     8221 tggatgccct tcgattcctg aggcaacagt ccgttcaggg catcctcatt agcaaccagt
     8281 cggtggtgaa ccgggggttc acgtccatcg aaaccctctg ggacatccac gaccggatgt
     8341 tggaccgtat tcggcgggcg ggtggagacg tcctggccgc gttttactgc ccccatcgac
     8401 cagacgaggc ctgtgcgtgc cgaaaacctt cccctcgaat gattctctac gccgccggga
     8461 tcttctccat tgaaacggcc gagacctacc tcatcgggga tcggagctcg gacatcctgg
     8521 ctggcgggga ttccggctgc aggaccgttc tcttggaaag accggactcg gaacaccccc
     8581 ccgagggggc atgggccggc cgacagcccg acgagcgata tccgtccctc ctggaggccg
     8641 tgacggcgct ctggaagcag gggaagctgt cgtgacgggg gctccgtccc cgccgacccc
     8701 atggcacccg aagaaatttc gtttccggaa ggagttccca tgttctatcc tccctttctc
     8761 ctgttttttc tgttcgcctt tctctttctt ctccttttcg cctttgcatt ggtccagttt
     8821 gggattttct cgtgggcctt caccaagctg gggatctcac cggagcacct tttctccctc
     8881 ctgtttcttt ccatcgtggg aagcatgatc aacattccca tccgaaagat tcccattgaa
     8941 accgacatgc aggacccgga catcgtttct tttttcggca tgcggttccg cccgccgcgc
     9001 cgacagagag tccgggagat ggtcctggcc gtgaacgtgg ggggcgctct gatccccacg
     9061 ggcctctcgg tctacctgtt cttccattcg cctcatccgt ttcggatgct gctggctctt
     9121 gccgcggtga cctgcgtggt gtaccgggcg gcaaagccgg tcccggggct tggaatcgcc
     9181 acgcccatgt tcattccgcc cctggttgcc gccctggcgg ccctcctgct ggacccccgt
     9241 tcggcaccgg ccacggccta tacggcgggc accctgggca cactcatcgg ggcggacctg
     9301 ctgcacctgg acaagctgaa ggaattgaga gccccggtgg cttccatcgg cggagcggga
     9361 accttcgacg gcatttttct tacggggatc ctggccgtac tgctcgcatg atccacaacg
     9421 cgaaccaccc ataacgtgag gggaactatg gcaagcaagg tgttttttat cgacctggcg
     9481 gtccgcaaca accagggact cctggaccgg attccgtccc tgcttgaggc ggcgggaatc
     9541 gccgaggtag tgcccgaccg cgcccttacg gccatcaaga ttcacttcgg ggagcgggga
     9601 aacaccgcct ttgtgcggcc gctgctggtg agacccatcg tggacgccgt cgccggggcc
     9661 gggggacggc cgtttctcac cgacgcgggg actctttacg tgggcacccg gggcaatgcc
     9721 gtggatcacc tgaataccgc catgttaaac gggtttgcct actcggtggt gggggctccc
     9781 ctggtcatcg ccgacggcct ggacggcggg gacgaagtgg cggtccccgt ggaactgaag
     9841 cactgccggg tagcctacgt ggcgtcggcc gtcgccaagg cccgggccct ggtgtccgtg
     9901 gcccatttca agctgcatga ggcggcgggc ttcggtgggg ccatcaagaa cctgggaatg
     9961 ggatcggctt cgcgcaaggg caagatggcg cagcactcgg actcgggccc cctgctggag
    10021 gcctccaagt gcatcggctg cggcctgtgc cgggagcagt gcgctcacga cgccatcacc
    10081 ctggtggatc gcgcggacgc cgaatcctcc ctgcctatgg cctccatcga ccatggcttg
    10141 tgcgtaggct gtgcccggtg cattcacacc tgtccccagg gggccctggg catcaattgg
    10201 gaaaaggatc tgcctcggtt catggaacga atggtggagt atacggcggc gaccctcaag
    10261 ggaaaggaag agaacctgct ttgcatcaac ttcctgaccc aggtctcccc cgcatgcgac
    10321 tgctatccgt tcgcagactc ccccgtggtg gccgacatcg gggtggtggc ctcccgggat
    10381 cccgtggccg tcgaccaggc atccatggac ctggttaatg cccaaccgcc cctggggtct
    10441 tcctgcatca aggactcgcc ccacgcttct cacgacaagg tgagggccgt ctacccccag
    10501 gtccagtggg aacaccagct ggcatacgcc gagcagttga agatgggaag ccggacctat
    10561 gatttggtga agctgtaagg ggcgggggat tgcggggtcc gggtcggcgg ggactttcgc
    10621 aagccggttt cgccggggag cccgcccggg gacggccggg attggaaatc gcacttgtca
    10681 aacggggaac ctttgagtaa atagatcact cggaagcgcc aagctcactg gtggggctcc
    10741 cggacttcaa atccggtgcg gggtagtgaa ggctgtcccg ggtgggttcg attcccatgc
    10801 gcttccgcca tcttacaggg acagccctct ttggagggct ttttgttttg gttacgcgca
    10861 cgaatgcagg tacccctaca acgccactta tgttccgcgc agcgatttcc tttttgcatg
    10921 agaacggtaa gcacggtgat ttctcgcctg aatccatgga acgatgacaa ggagatcctc
    10981 gccggcaagg accctcatgg gcaatagggc ttgaaagagg aatctaagct gcgacagtgt
    11041 aatgcacggt gctttttttc cccgatttga tgctgaggtg ccggaggaag aagtgagcca
    11101 gcatgcaggt gagcatgtga tgattccatc ccggatattt cctgacctca tagtgatcca
    11161 tgcccagttc ggattttgcc tcttcgaagc attgctcgat agcccagcgc acgccgctga
    11221 gccatacgaa gagctccagt cgcgcgctca ccggtgcgtt gctgatatag taggaatact
    11281 cccggttggt gccgagagtt cttttgataa cgagccaaac cgtcttgtcg gggagccctc
    11341 ctttgcagag ggtgacgcgt ctcttggtaa attcatattc aatgggtccc ttggttccct
    11401 cggaaacttt gcggcgatac caaaagaagg aatgaatcag gccggccaac gtcgcaaaat
    11461 ccaccggctt cttttcgcca tcggaaaccg cccgtctgac tttcgtgccc cctttgccac
    11521 ggaccgctct ttgttcggtg gacggtccct gcaaccagca caaggtgtcc gacggcatcg
    11581 agacaaagta agttgtaccc acacgccctt caacggcgcc aatgaagtcc ggattggcgc
    11641 catatacgga gtcggcgacg acgtacttga aagggacgat accctcggcg cgcaggtctc
    11701 ggtacatctc cgcggccagt tgaggcttgg tctgaaaggt taactcgacg ggcattctgc
    11761 attttcggcg tcgctccgag tgggtctctt cgaaccagtt gcgaggtagg taaagccgct
    11821 tgtccagaaa ggcatagcca tgacgagatg cgtaggcggc gaaaactccg acctggcaat
    11881 tgtcgacctt cccggtgctg ccgcaatatt gcctggcgac cccgatcgag tcatcccctt
    11941 tcttggcgaa tccggattcg tcaaggatca gcaccccttc gggatccccc atgtcttcag
    12001 cgaccagggt atggtatcgg cgaagcatga gcggttcatc ccacactacg tcgcttacaa
    12061 aacgctgcat cgcgcgaacc ttgccgtcct cgatctgaag cgcgatcggc tcgatggact
    12121 ttctctccag cgggctgaac tgaccggaca tgtaaagccg gaaatgctct ctgggttcgc
    12181 ttctcgtgaa gcaatctcga aattgttcgt ggaatccacg aagttcgtcc atgaatcctt
    12241 ccacgtcatc cctttccgga gagaactttg gaatgggata tgaatcgcca tgacaacggg
    12301 attccgaccg catagcccac ctcctttacc atcgtagtcg ggttgaggtg gattatgcaa
    12361 caaaacgaaa gtgggttcaa gatttatatg tcgttgtagg gctatgtttc tgccaccgct
    12421 aataaatcca atgctcttca atccatgcaa agaacaaggg acgaggggct aaactgcaaa
    12481 acttcagtta catgcattct gccaaagttc agttttcagt agcttgacaa ccgggctggc
//
LOCUS       DAOOVC010000111        12451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3338_length_12451_cov_7.294773, whole genome shotgun sequence.
ACCESSION   DAOOVC010000111 DAOOVC010000000
VERSION     DAOOVC010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12451
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_12451_cov_7.294773"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLM79_13020"
     CDS             <1..429
                     /locus_tag="PLM79_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV87279.1"
                     /translation="FAGAAAGFTLPEFRTLAGHAAENVFSATLWTPQVPYPGARQYYQ
                     SYLKRFGSEPDYHGAEAYASVYVVADALKRAKELTPGGVREALAKTDMMTAFGPVRFT
                     SYDNKTQQNSLPTFLVQWQKGALETVWPRTVATKTYVFSE"
     gene            complement(658..3006)
                     /locus_tag="PLM79_13025"
     CDS             complement(658..3006)
                     /locus_tag="PLM79_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV87280.1"
                     /translation="MKIFSRGIAGKILGGSTILILVGVVVGGAGYISLDRVIRAGNMS
                     TSANDVQAKILEARVLEKEFILTKDKQVYEKLVRTLGELEKSSSQLLAAGTESRGVEE
                     IRQAGQVYEKAAAELKRLEEEDAAVLTELQKVAASISSIAAEESSKALAKTKSDILQA
                     NTETLREYSREKIRGVVSVGHDVLKHYHDVGISQAAALDALRNMHFDGDNYFFVVQEN
                     LTLVAHGSNRKLEGMDFGKIQDKKTGKTFMREVVDAAMKDGESLTEYYWTKPGAGEAI
                     FPKLTYGKYFKPWGLIVCAGVYVDDIEKQIHRTAGLFESGIDRLQQANAINDLTLQAR
                     LNALYYFAFATNAEKVAEKISLLTNLKVATDALKKDAGAYLEKFNQRVANNEKRRQEI
                     GRINEAAERTLKASTGISSGAMDTFGKSVSGGKGMIIAFILVGLVGGFVLAFFLARGI
                     THPINRAMAGIEEASEQVRAASEQVESASQELAEGSSEQAASLEETSSALEQMAAMTR
                     QNADNANEATKLMAESGRVVEQANQSMSLLTASMGQITEASEQTQKIIKTIDEIAFQT
                     NLLALNAAVEAARAGEAGAGFAVVADEVRNLAMRAAEAAKNTADLIEGTVKRIKEGSQ
                     LVEKTNAEFNQVTGSVIKSGELVREIAAASQEQSQGIGQVSHAVAEMDKVTQKSASSA
                     EESASASQQMKAQAVQLRGYVEDLARLVGGTVQGALSLSGKEKAAVTRVLSVSAAPPS
                     PVPGAPPQRKGNGKALLSAASLFRRGKTQPLLPQDDHEYRDF"
     gene            3509..3952
                     /locus_tag="PLM79_13030"
     CDS             3509..3952
                     /locus_tag="PLM79_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87281.1"
                     /translation="MVSDRDSWLCGQIVEKAQDGVIYSDREGLIRLWNSGAERIFGYS
                     TGEALGQSLDLIIPEPLRARHWEGYGKVMETGKSRYESDLLAVPALRKDGSRISIEFT
                     VVPILDRSGQMDGIAAVIRDVTDRWLREKATRQRLAQLEAQCSKE"
     gene            4209..5945
                     /locus_tag="PLM79_13035"
     CDS             4209..5945
                     /locus_tag="PLM79_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOV87282.1"
                     /translation="MHELDLLPNIGLAIVAATFFGLLAKILKQPLLLAYLAAGIALGP
                     RMGFGLIEDEASIILIAEIGLILLLFIIGLEIDLKRLLSAGRTLLVSGLSQFPICVAM
                     GSGFFVLLGFKMGGDRFDALYLAVAMALSSTMIVVKILYDKFELSTLPGRITLGILVF
                     QDIWAILFLALQPNLLNPGASILLLSLAKGAGLVAFSLLMSRYVLPHVFTVIAKVPEL
                     LLVTAIAWCFLISGIAEQMGLSMEMGALVAGVSLSTFPYNVDVIAKVINIRDFFVTLF
                     FVGLGLQIPMPTASLLGYAFVASFFLVATRFFSIFPILFSLGNGLRASLIPSVNLSQM
                     SEFSLVIASIGLSLKHIDPQVVGILTFVFAITSVASTYMIQYNHEIQQKMAPVLQRLG
                     CRDIDRETAGDEEAGRPKEIVFLGFFREASSIFHELEVLGNGEDLALTEKILVVDFNP
                     LVHSELNKRGVSCTYGDISSADTLKHAHIDGAKTLVCTIPDSILRGTTNEKLLLMSRR
                     LCPGSRVIVTANDLQTAMRLYEQGADFVFIPRIHSARQAARIISSSLREDLERYREAE
                     MTNLRERNEILG"
     gene            6125..6547
                     /gene="rnk"
                     /locus_tag="PLM79_13040"
     CDS             6125..6547
                     /gene="rnk"
                     /locus_tag="PLM79_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HOV87283.1"
                     /translation="MAKKRTIYVTEFDLNRLNKLIEALGDEPEMRDRKYLEELDEELH
                     RARVVPPKSIPRDVITMNSKVRVRDLDSGTETTYQLVFPGDANVQQNKISILAPIGTA
                     IIGYRAGDVVEWEVPAGRKRMKIEEILYQPEAAGDFHL"
     gene            complement(7195..8364)
                     /locus_tag="PLM79_13045"
     CDS             complement(7195..8364)
                     /locus_tag="PLM79_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV87284.1"
                     /translation="MFLLTQYSYRNLWVRRLTTLLTSGGMGLVVFVFSAVLMLADGLE
                     KALVSTGSRDNVVFIRRSAEAEVQSIIERDQAQILESLPEIDTNSLGQKLVARELVVL
                     ISLQKLQTRSLANVLVRGVNPQFSRELRPQIRMVSGRPFHPGAAEIVIGKNIAQRFSV
                     GGLGQSLSFGMRDWTIVGVFDAEGTGFDSEVWGDVDLMMASFRRPVYSSMIAKLRDPS
                     DFEILRNRVLDDPRLTVEAKRETSYYAAQSELMARFIRILGITLTLIFSMGAVIGSMV
                     TMYAAVSYRTAEIGTLRALGFRRTTILLAFLTESVFVSFIGGLIGLGAASFMHRLTIS
                     TLNWQTFSELSFQFTLSTSIARSALLFATTMGLVGGLLPALRAACLNIVTALRYR"
     gene            complement(8416..9687)
                     /locus_tag="PLM79_13050"
     CDS             complement(8416..9687)
                     /locus_tag="PLM79_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87285.1"
                     /translation="MNQVAEKHWQYLSRVLEESIRALNARETEAFDGSVSKFLDASGT
                     LGLTELAAMADKFAQFRSGHLTQGWNEEAAATLSFSMGALLEKMQTTSYGPEFVSGLG
                     EVSLYLDFFAEADSVEPTKEKEEFPESPGPERRIGPEIEIAPSADGRDGPLRSPSAAD
                     LAAPLCEPQEPGETNRVSVPSAAVSPETEAKESAGRAPWTAQPSIPVALDPPSVIPAA
                     QTGTGSRAEAVDLSEDPPGYVMDIFDWYKELLDFDPASPAFEFLAEEFCLKGMWKEAS
                     EVCRRGLVHHPNHLRGRILLGWALWKLGEGEESEIILQTARKEIEKNALLYRVLAEIA
                     DHRRQRELSLSFLDLYHRLCPRDREEPSAVPQSPELPEGLPGPEEGVLGILEAWMLGL
                     ETLPAGPSPKRVLFDDSDREELRGLLKKALH"
     gene            9961..11313
                     /gene="mgtE"
                     /locus_tag="PLM79_13055"
     CDS             9961..11313
                     /gene="mgtE"
                     /locus_tag="PLM79_13055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOV87286.1"
                     /translation="MVDKLKQEQVRQWIRDENREALLHAFEDLHPADAAEQLAGLSPS
                     EVARYVELVGPAETLPIFEFVPFEMQRETLEHLPRSILPRLIELMSPDDRADLVEELD
                     EKSQERLLSLLMQAERQELLKLITYPEGSAGGHMTTDYAFLRPDVSIGTALDHLRMQA
                     PKKETIYYVYVVDLARRLIGFLSLRDLIMEHPERYVREVMNKNVIAVRVDQDIEEVAK
                     VMSRYDFLAIPVVDAEQRLVGIITYDDVFDVMAEEATEDMYLLANLDTDEKITSPLPR
                     SVKLRVPWLLVNLCTALLAAYTVSLFSDTISAFVTLASLMPIVAGIGGNAGTQSLTVV
                     VRALALGQIKVHGNLPILLKQVGIGLFSGLICGFVMGAVAYLWHHNIWLSLILWMAMT
                     ANLVIAGSFGAIVPITLRKFNLDPALGSSIFVTMATDVGGFLTFLGLATLLLHHLMGP
                     "
     gene            complement(11366..>12451)
                     /locus_tag="PLM79_13060"
     CDS             complement(11366..>12451)
                     /locus_tag="PLM79_13060"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOV87287.1"
                     /translation="QLNWFPDVFHLHDWQSALVAPYQHVHWRYDPNFSGSSVVFTIHN
                     LAYQGLFPARQFALTRLPSEVFTIQGMEYWGQCNLLKAGLVYGDAITTVSPRYSREIQ
                     QPEFGHGLDGVLRDRKDRLLGILNGIDYSQWDPATDPLIPQRYSARDLGGKGECKKDL
                     LSDLGFGQESRERPLLAMIGRLTPQKGFDLLAAVLDEFSALPVSLVVLGAGDPELEDR
                     LRRCADELPGQVRTIFRFDERLAHRIEAGADIFLMPSRYEPCGLNQMYSLRYGTVPVV
                     HATGGLDDSVTDVLRDPEGGTGFKFYEYTPSALMSAVRSALEWYEDRGRWEELQRRGM
                     AQDFSWNRSARQYLEVYRTVAERKKRL"
BASE COUNT         2440 a         3805 c         3612 g         2594 t
ORIGIN      
        1 ttcgccggtg cggctgccgg ctttaccctg cccgagtttc gcacccttgc gggccatgcg
       61 gcggaaaacg ttttctccgc gactctctgg accccccagg tcccttaccc gggggcgcgc
      121 caatattacc agagctacct gaaaagattc ggatccgagc cggactacca cggtgccgag
      181 gcttatgcct ccgtctacgt ggtggcggac gccctgaaga gggcgaagga gctgaccccc
      241 gggggcgttc gcgaggccct ggcgaaaacc gacatgatga ccgctttcgg gcccgtgagg
      301 ttcacgtctt acgacaacaa gacccagcag aactcgttgc cgactttcct ggtccagtgg
      361 caaaagggag ccctggagac cgtctggccc cggaccgtcg ccaccaagac ctacgtgttc
      421 tccgaatgaa cattgccggg aagaggcttt gggccccggc gcccccgagt ggcatacgcc
      481 tcacaaggat cagacacttc cggcggcttg cccctgcagg acgaccgggg ctttccgctt
      541 ccccgagcgg tgattgaggg ggtggggagc ggattttgga ggagggatca aaagaatccc
      601 ccggcctcca agaccggggg atctgtcctt tcctcgccgc acccgtttga agcgcggtta
      661 gaagtcccgg tactcgtggt cgtcctgggg aagaagcggt tgagtctttc ccctgcgaaa
      721 cagggacgcg gcagacagta gggccttgcc gttgcctttt cgctggggag gtgcaccggg
      781 cacaggggaa ggaggggccg cagaaaccga caggacacgg gtcacggcag ccttttcttt
      841 cccggaaaga ctcaacgccc cctggacggt acctcccacc aatcgggcca aatcctcgac
      901 gtaacccctc aactggaccg cctgggcctt catttgctgc gatgccgatg cggactcctc
      961 agcactggac gcactcttct gagtgacctt gtccatctcc gccacggcat ggctgacttg
     1021 gccgattccc tgggattgct cctgagaggc ggcggcaatt tcgcgaacca actccccgga
     1081 ttttatcacg ctccccgtca cctggttgaa ttcggcgttg gtcttttcaa ccagttggga
     1141 gccttccttg atcctcttta ccgttccttc aatgaggtcg gccgtgttct tggccgcctc
     1201 cgccgcccgc atggcaaggt ttcgcacctc atcggccacc acggcgaacc cggcccccgc
     1261 ctcaccggct cgagccgctt ccacggcggc gttcagagcc agcagattgg tctggaaggc
     1321 gatttcgtca atggtcttga tgatcttctg agtctgctcg ctggcctcgg tgatttgtcc
     1381 catggaggca gtgagaagag acatgctctg gtttgcctgt tccaccacgc gaccggactc
     1441 ggccatgagt ttggttgcct cattggcgtt gtcggcgttt tggcgggtca tggcggccat
     1501 ctgttccagg gccgaggagg tttcctcgag ggaggccgcc tgctccgaag acccctcggc
     1561 cagctcctgg ctggcactct ccacctgctc cgaagccgcc ctcacctgct cggacgcctc
     1621 ctcgatgccc gccatggctc gattgatggg atgggtgatc cccctggcca ggaagaaagc
     1681 caggacgaat ccgcccacca aacccacgag aatgaaggca atgatcatcc cttttccccc
     1741 ggagacactc ttgccgaacg tgtccatggc cccggagctg atccccgtgg atgccttgag
     1801 ggtcctctcc gccgcttcgt tgatccggcc gatttcctgc cgacgtttct cgttgttggc
     1861 cacccgttga ttgaatttct cgaggtaagc gcccgcatcc tttttcaaag cgtccgtggc
     1921 caccttaagg ttcgtgagaa ggctgatttt ctccgcgacc ttctccgcgt tggtggcgaa
     1981 ggcgaaataa tacaaggcgt tcagccttgc ctggagggtg agatcgttga tggcattggc
     2041 ctgctgcaac cggtcgatgc cgctctcgaa aagccccgcc gttcgatgga tttgtttttc
     2101 gatgtcgtcg acgtagacgc ccgcgcatac gatcaagccc cagggtttga agtacttccc
     2161 gtaagtgagc ttggggaaga tggcctcccc cgctcccggc ttggtccagt aatattccgt
     2221 gagagactca ccgtccttca tggccgcatc caccacttcc ctcatgaagg tcttgccggt
     2281 cttcttgtcc tggatcttgc cgaaatccat tccttccagt ttccggttgc ttccgtgggc
     2341 gacgagggtc aggttctcct ggaccacgaa gaagtaattg tctccgtcga aatgcatgtt
     2401 tcgcaaagca tccagggctg cggcctggga gatgcccacg tcgtgatagt gtttcaaaac
     2461 gtcatgcccg acggacacaa cccctcggat cttctctcgg gagtactccc tgagggtctc
     2521 ggtgttggcc tgcagaatat ccgatttggt ttttgccaga gccttggaag attcctccgc
     2581 ggcaatggaa ctgatgctcg cagcgacttt ctggagctcc gtcagaaccg cggcatcttc
     2641 ctcttcgagc cttttcagct ctgcagccgc tttctcataa acctgacctg cctgccggat
     2701 ctcctcgacc ccgcgactct ccgtcccggc ggccaggagt tgggaggagg atttctccag
     2761 ttcgcccaaa gtccggacca gcttttcgta gacttgtttg tcctttgtga gaatgaactc
     2821 cttttccaag acccgggctt ccaggatttt tgcctgtacg tcattggcgg aggtactcat
     2881 gttgcccgct cggatgaccc tgtccaggct gatgtaaccc gctcctccga cgacgacccc
     2941 aaccaggatc aaaatcgtgg agccgcccag gattttccct gcgattcccc tgctgaagat
     3001 cttcatgccc ctgccctccc tcggttggtc ttccctgcaa gtgcctcctc tgttaccctc
     3061 tgccgaaaac gattccgatt cccctgcatc cgcaccttgc ggcgctatcc tctccccttc
     3121 gcttttgggc gggtcgcgga ggggatggga aaccgcccaa gaacagcatt cgacacaaca
     3181 tgtggcggcg tcggttccaa gagccttctc atcgcaatcg atctacggtg tgggaaggcg
     3241 ctccgcccga gaaacccatc cgtggatcag gcaacaaccg tcgttgggaa cattcatctt
     3301 gggtcacgtt cacgggaacg atgggttttg ctctaaccta tcggcgtagc gcatcgattt
     3361 gattagacaa attttcgaaa aaaaatcgag aaacctcccc gagcttgaat ccgtcgggtt
     3421 ccctctcggg attctgatgg caaaagacac gggggaaggg tattctccat atccgtgagg
     3481 aaacagcgga cctgtaagga gattcaacat ggtgtcggat cgagattcat ggttgtgcgg
     3541 acagattgtg gaaaaggccc aggacggcgt gatttattcc gatcgggagg gactcatccg
     3601 gctgtggaac tccggagccg agcgcatctt cgggtactcg acgggggaag ccctggggca
     3661 atccctggac ctcatcattc ccgagccgct gagggcgcgt cattgggagg gctacggaaa
     3721 ggtgatggag accggcaaga gtcgatatga atccgatctt ctcgcggttc cggcgctcag
     3781 aaaggacgga agccgcattt ccatcgagtt taccgtcgtg cccatcctgg atcggagcgg
     3841 gcaaatggac gggattgccg ccgtcatccg ggacgtgacc gatcgttggc tgcgcgaaaa
     3901 ggcaaccagg cagcggttgg cccaattgga agcgcaatgc tcgaaggaat aatccccctc
     3961 atgggctgaa aacgaaaccg acccgttttc ccgcgaggac tccgccgtcg cgcccggggc
     4021 gaacggacgg acccgcggcc tccccgacga agcccttgac caagcctgcc acatttgcaa
     4081 tgaatgaagt gtccgggtcc gccacggcca aatcggccgt gcagcggaca cgaccgttcc
     4141 cgaaaatcgg gacggcggcc tgtcttgtcg ggaaatgagc cgtcggttcc tctgaaaggg
     4201 tttttgcgat gcatgaactg gatttgttgc cgaacatcgg cctggcgatt gtggccgcca
     4261 cttttttcgg tctcctggcc aagatcttga aacagccttt gctgttggcg tacctggcgg
     4321 cggggatcgc cctgggaccg agaatgggat tcgggttgat cgaggatgag gccagcatca
     4381 ttctcattgc ggaaatcgga ctgatccttc ttctcttcat catcggcctc gagatcgacc
     4441 tcaagagact cctctctgcc gggcgaactc tcctggtttc gggcctttcc cagtttccca
     4501 tctgcgtggc gatgggcagc ggcttttttg tcctcctggg cttcaagatg ggaggagacc
     4561 ggttcgatgc gctttatctt gccgtagcca tggcgctgag cagcacgatg atcgtggtga
     4621 aaatcctcta cgacaagttc gagctctcca ccttgccggg gcggattacc ttgggcattc
     4681 tcgtgttcca ggacatctgg gccattctct ttctggccct ccaaccgaac ctgctgaatc
     4741 ccggagcatc gattctcctg ttgtctttgg caaaaggagc gggactggtg gccttcagcc
     4801 ttctcatgag ccgatacgtt ctgccgcacg tctttacggt catcgccaag gtccccgaac
     4861 tgctgctggt caccgccatc gcctggtgtt tcctcataag cgggatcgcg gaacagatgg
     4921 ggctttccat ggagatggga gccctggtgg ccggggtgag tctgtccacg tttccctaca
     4981 acgtcgatgt gatcgccaag gtgatcaaca tacgggactt ttttgtgacg ctgtttttcg
     5041 tcggcctggg tctccagatc cccatgccca cggcgtccct cctcgggtat gccttcgtcg
     5101 catccttctt tctggtcgcc acccggtttt tctccatctt ccccattctt ttctccttgg
     5161 gaaacggcct gcgggcgagt ctcattccct ccgtcaacct ctcgcaaatg agcgagtttt
     5221 ccctggtcat tgcctccatc gggctgagcc tgaagcacat cgatccccag gtggtcggga
     5281 ttctgacctt cgtcttcgcc atcacttcgg tcgcctcgac ctacatgatc cagtacaatc
     5341 acgagatcca acagaagatg gcgccggttc tccagaggct cggctgcagg gatatcgaca
     5401 gggaaacggc cggtgacgag gaggcaggcc gaccgaagga aatcgtattc ctgggattct
     5461 tcagggaggc gagttccatc ttccatgagc ttgaggtcct cgggaacggg gaagacctag
     5521 ccctgaccga aaagatcctg gtcgtggact tcaatcccct ggtccacagc gaactcaaca
     5581 aacggggggt ctcctgcacc tacggcgata tttccagtgc ggacaccctg aagcacgctc
     5641 atatcgacgg ggccaagacc ctggtctgca ccatcccgga ctccatcctt cgaggcacca
     5701 ccaacgagaa attgctgctc atgtcgagac gtctctgtcc aggctcccgg gtgatcgtca
     5761 ccgccaacga cttgcagacg gcgatgcggc tctacgagca gggggccgac tttgttttca
     5821 tcccgagaat ccattccgcc cggcaagcgg cccggatcat ctccagctcg ttgagggaag
     5881 acctggagag gtaccgagaa gcggaaatga cgaaccttcg ggagcgaaac gaaattcttg
     5941 gctgaggcaa gaaggtcctt cccctggtcc gcgtccggga accccggctc gaggggggaa
     6001 gcatgcgccg accggctaac ggcaaggtct tcggcttcga gggaaagcga ttcggacccg
     6061 gcaacgacca tggatccccg ggaagcgacc tcgggtccgc cggaatcgaa aggagaggca
     6121 acgtatggca aagaagagaa ccatctatgt cacggaattt gatttgaacc ggctcaacaa
     6181 gctgattgaa gcgctgggag acgaaccgga gatgcgcgat cgcaagtacc tggaagaact
     6241 cgacgaggaa ctgcaccggg ccagggttgt gcctcccaag agcattcccc gggatgtgat
     6301 caccatgaat tccaaggtcc gcgtgaggga tctggactcc gggacggaga cgacctacca
     6361 gctggtattt cccggggacg cgaacgtgca acaaaacaag atctccatcc ttgcccccat
     6421 cggcaccgcc atcatcgggt accgcgccgg agatgtggtt gaatgggaag ttccggcagg
     6481 gcgaaagaga atgaagatcg aagaaatcct gtatcagccc gaagcggcgg gggactttca
     6541 cctttagccc gaacccccgg tggcgggcgg gcgcggctgc ctgggcgatt cccggttcgg
     6601 cccttgggga tctcctttca cgggcgccca cgggaatccc ctcaccagga tgtgtcttcc
     6661 cgcaggatcg gcctcctcga ggagttcctc catggggtgc tggcctagga gggccacgtt
     6721 cttgagccct ttccctgcag gacacccttt cggaaacctt cgagggtggt cccacaagcc
     6781 ctacaaccga cggggacagg tgcatctcga gccgagacct tggtgagctc ccggaaggtg
     6841 gccaggtctc cctccgccct caggagggcc aggtaggggt cgctcatccc ctctcaccaa
     6901 atggaacggg aaagaaggct gcgttgcgca gcttcctgtt gctccgggca agccccgccc
     6961 tcatttccgc ctgcttgatt tccctcttta cacggcggct tccgcaaggc tcttcatggc
     7021 gggatgggcc cgactctcta tggagtcccc ctcttgtccc aatcgctccg gtcgtccccc
     7081 ttgcattgcc gggattgcag gggacctgct cgtggcatgc gaatccgagt atttcagaaa
     7141 aactcagggg gtcccgatgc gttccgggtt gaaggctttc cattcctcga accttcaacg
     7201 atagcgcagc gccgtgacga tgttcagaca agcggcccgc agggctggaa gcagccctcc
     7261 caccaggccc atcgttgtcg caaagagcag agcgcttcga gcgatggagg tggagagggt
     7321 gaattgaaaa gaaagctcgg aaaacgtctg ccagttcagg gtggagatgg tcagccgatg
     7381 catgaaggat gcagcgccca gaccgatcaa ccctccgatg aaactcacaa aaacggattc
     7441 cgtcaggaag gccaggagga tggtcgttct gcgaaacccg agggcgcgga gggtcccgat
     7501 ttccgcggtt cgataggaga ctgcggcgta catggtcacc atggagccga tgacggcccc
     7561 catgctgaag atcagggtga gcgtgattcc caggattcgt atgaaacggg ccatgagttc
     7621 ggactgagcg gcataatagc tggtttcccg tttcgcctcc acggtaagcc tcgggtcatc
     7681 gaggacccga ttccgcaaga tttcgaagtc ggaagggtcc ctgagcttcg cgatcatcga
     7741 agaatagaca ggacggcgga aagaagccat catcaggtcc acatctcccc acacttccga
     7801 atcgaatccc gtcccctcgg catcgaaaac gcccacaatg gtccagtctc gcatgccgaa
     7861 actgaggctc tggcccaacc ctcccacgga aaacctttgg gcaatgttct tcccgatgac
     7921 aatctccgcg gccccgggat ggaacgggcg gccggagacc atgcggattt ggggccgaag
     7981 ctctcgggaa aactgcggat ttaccccacg gacgagcacg ttggcaaggc ttcgggtctg
     8041 aagcttttgc aggctgatta aaacgaccag ttcgcgggcc accagcttct gacccaggct
     8101 gttggtgtcg atttccggca ggctttccag gatctgtgcc tggtctctct cgatgatgct
     8161 ctggacttcc gcctccgcgg acctgcggat gaaaaccacg ttgtcccgac tgcccgtgct
     8221 caccaaggcc ttttcgagac cgtcggccag catgaggacc gccgagaaga cgaacaccac
     8281 caatcccatc ccccccgagg tcaggagggt ggtcagcctc cgcacccaca gatttctata
     8341 gctgtattga gtcagcagga acatgagagg cggtgctcgt tcctttgaga cagtgtggcc
     8401 gggcgtttcc cgagctcaat gcagggcctt tttcagcaac cctctcagct cttcccgatc
     8461 cgaatcgtca aacaggaccc gtttcgggga agggccggcg ggaagtgttt ccagtcccaa
     8521 catccatgcc tccaggatgc ccaggacccc ctcctccggc cccggaagtc cttcaggaag
     8581 ctcaggagac tgcgggacgg ccgaaggctc ctcccggtcc cggggacaga ggcgatgata
     8641 gaggtccagg aaggaaaggg acagttctct ctgtcttcgg tgatctgcga tttccgccag
     8701 gactcggtac aggagggcgt tcttctcgat ctccttgcgc gcagtttgca ggatgatctc
     8761 ggattcctcc ccctcaccca atttccatag agcccatccc agcagaatcc gaccccggag
     8821 atggttcgga tgatggacga gaccccgacg gcagacttcc gacgcttcct tccacattcc
     8881 tttgaggcag aattcctccg ccaggaattc gaaagccggg gaggcgggat cgaaatccag
     8941 gagctctttg taccaatcaa aaatgtccat cacatagccg ggggggtcct cggaaagatc
     9001 gacggcctcg gcacgggacc ctgtcccggt ctgcgccgca ggaatcacgg aaggaggatc
     9061 gagggcaacg gggatcgaag gctgggcggt ccatggagca cgacccgccg actccttggc
     9121 ttccgtttcg ggagacacgg cggcagaggg caccgagacc cgattcgtct ccccgggctc
     9181 ttggggctcg cagagaggag ccgcgaggtc cgcagcactc ggagaccgca gaggaccatc
     9241 ccgtccatcc gcggacggtg caatttcgat ctccggaccg attctcctct cggggcccgg
     9301 ggactcggga aattcctcct tttctttcgt cggctcgacc gagtcagcct cggcgaagaa
     9361 atccaggtaa agggaaacct cccccagtcc gctgacaaat tccggaccat agcttgtggt
     9421 ctgcatcttt tccagcaagg cgcccatgga aaagctcaag gtcgcggcag cctcttcgtt
     9481 ccagccttgt gtgagatggc ctgatcgaaa ttgcgcaaat ttgtccgcca tggctgccag
     9541 ctccgtcagg cccaacgtcc cggacgcatc caggaacttg ctcaccgacc catcgaaggc
     9601 ctcggtctct cgggcattga gggctcggat ggattcctcg agtacacggc tcagatactg
     9661 ccagtgcttt tccgccactt ggttcattcc ccggcctccc gccttggatg ggttaatcga
     9721 tcacgccgaa ccacccaggg atttaacggt atcgtccttc cagggtcaag gaagaaaatg
     9781 gaacggcttc ggcgacggac gccccatcct cctgcagatt ccccgacaaa tcattgctca
     9841 gctgccgaaa ggtccgtatt cgttgaaaag caccctccgt tctgctacta atgcaacctc
     9901 cccgcaggaa caaggttggt agagcactct cctatcttta aggaagtccc ggagacggcc
     9961 atggtggata aactgaagca agaacaggtg cgacaatgga ttcgagacga gaatcgtgaa
    10021 gcccttctgc acgcatttga agatctccac ccggcggacg cggccgaaca actggcgggg
    10081 ctcagcccct cggaagtggc ccgatacgtg gagttggtgg gaccggctga gacgctgccc
    10141 attttcgaat tcgtcccctt cgaaatgcag agagaaaccc tggaacacct tcctcgatcc
    10201 atcctgcctc gactgatcga gttgatgagc ccggacgatc gagccgacct ggtggaggaa
    10261 ctggacgaaa agtcccagga gagactgctc tctcttctca tgcaggccga gaggcaggaa
    10321 ctcctcaagc tgatcacgta tccggaggga agtgccggtg ggcacatgac cacggactac
    10381 gcgttcctcc ggcccgacgt gtccatcgga accgcactgg atcacttgcg gatgcaggcc
    10441 cccaagaagg aaaccatcta ttacgtttac gtggtggacc tggctcggcg gctcatcggt
    10501 ttcctgtctc tccgggacct gatcatggaa cacccggaac gctatgtgcg ggaagtgatg
    10561 aacaagaacg tcatcgccgt gagggtggac caggatatcg aagaggtggc gaaggtgatg
    10621 agccggtacg actttctggc cattcccgtg gtggacgccg aacagcgcct ggtagggatc
    10681 attacctacg acgacgtgtt cgacgtgatg gcggaagagg ccacggaaga catgtacctc
    10741 ctggccaacc tggatacgga cgagaagatt acctctccct tgccccggtc ggtgaaattg
    10801 cgggtcccgt ggctgctggt caacctttgc acggcgctcc tggcagccta caccgtgagc
    10861 ctgttttcag acaccatcag cgcatttgtg accttggctt ccctcatgcc catcgtggcc
    10921 ggaatcggag gcaacgccgg cacccagtcc ctgaccgtgg tggttcgagc cctggccctg
    10981 ggacaaatca aggttcacgg gaacctaccc attctgttga aacaggtggg aatcgggctt
    11041 ttcagcggcc tgatctgcgg gttcgtcatg ggggcggtgg cctatttgtg gcaccacaac
    11101 atctggcttt ccctgatcct ctggatggcc atgacggcca acctggtcat cgccggatcg
    11161 ttcggggcga tcgttcccat cacccttcga aagttcaacc tcgatcccgc cctgggttcc
    11221 agcatctttg tcaccatggc cacggatgtg gggggattcc tcacgtttct gggactggca
    11281 accctcctgc tccatcacct catgggaccc taggcccaag gcatggagcc tcgagacggc
    11341 ttcccgccgg ggcggcggcg gattttcaca gcctcttctt gcgttccgcc accgtccggt
    11401 agacctcgag gtactgccgg gcactccgat tccaggagaa atcctgagcc atcccccttc
    11461 tctgcaactc ttcccatcgc ccccggtctt cgtaccactc caatgccgac cggacggcgc
    11521 tcatgagggc cgatggagtg tactcgtaaa acttgaagcc cgtcccgcct tcgggatccc
    11581 gcaggacgtc cgtgaccgag tcgtccagcc cgcccgtggc atgcaccacg ggcacggttc
    11641 cgtaccgcag actatacatc tggttgagac cgcagggttc gtagcgggac ggcatgagaa
    11701 agatatcggc gccggcctcg attcgatggg ccagccgttc gtcaaatcga aaaatggtcc
    11761 gaacctgccc cgggagctca tccgcgcatc ttcgcaggcg atcttccagc tccgggtctc
    11821 cggcacccag gaccacgagg gaaacgggca gagccgaaaa ttcgtccaga accgccgcga
    11881 gaaggtcgaa gcctttctga ggggtgagcc tgccgatcat ggccagcagc ggtctttctc
    11941 ggctctcttg cccgaatccc aggtccgaaa gaaggtcctt cttgcactcg cccttcccac
    12001 ccaagtcccg ggcggaatac cgttggggaa tcagcgggtc cgttgccggg tcccattggg
    12061 aatagtcgat cccgttgagg attcccagga gccgatcctt tctgtcccgg agcactccgt
    12121 ccagaccgtg gccgaactcg ggctgctgga tttcccggct gtagcgcgga ctgacggtgg
    12181 tgatggcgtc gccatagacc aggcccgcct tcagaaggtt gcattggccc cagtattcca
    12241 tcccctggat ggtgaaaact tcggagggaa gacgggtgag ggcgaattgc cgggcgggga
    12301 agagtccctg gtaggccagg ttatggatgg tgaagaccac ggaggacccg ctgaagttgg
    12361 ggtcatatct ccagtgaaca tgctgataag gagccaccag cgcgctttgc cagtcgtgaa
    12421 gatgaaacac gtcgggaaac cagttcaact g
//
LOCUS       DAOOVC010000112        12344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3363_length_12344_cov_6.605257, whole genome shotgun sequence.
ACCESSION   DAOOVC010000112 DAOOVC010000000
VERSION     DAOOVC010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3363_length_12344_cov_6.605257"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(593..669)
                     /locus_tag="PLM79_13065"
     tRNA            complement(593..669)
                     /locus_tag="PLM79_13065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(633..635),aa:Met,seq:cat)
     gene            complement(846..1439)
                     /gene="hisB"
                     /locus_tag="PLM79_13070"
     CDS             complement(846..1439)
                     /gene="hisB"
                     /locus_tag="PLM79_13070"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOV87288.1"
                     /translation="MSNERKSRIERETKETRIETEWSLDGQGIAEIQSGVPFLDHMLT
                     LFAVHGFFDLKIRARGDLEVDAHHTVEDMGICLGRALSAALGDRKGIRRYGSAVVPMD
                     EARATVVLDLSNRPYLVWEVPDLPDRVGEFETELVPEFFRAFCHHGGVTLHITVSCGR
                     NTHHILEAVFKAWGRALDRAVGLDPRRTEVPSSKGSL"
     gene            complement(1506..2432)
                     /locus_tag="PLM79_13075"
     CDS             complement(1506..2432)
                     /locus_tag="PLM79_13075"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOV87289.1"
                     /translation="MRIVVIGQAAFGEKTLEALLEKGENVVGVFTPPDKPGGKADPLK
                     SLAASRNIEVIQPAAYRDEAVFDRYRDLKPDLAILAFVTAIIPARYFEAATHGAICYH
                     PSILPRHRGASAINWAVMMGDTRTGLTIFWPDGGIDTGPILLQKEIEIRPEDTTGSLY
                     FNHLFPMGIQAIVESVEMIKEGRAPRIPQKEEGATYEPPCDDRVARIDWNRSAVEIYD
                     WIRGCDPQPGAYSYLKGEKIRFFGAKLVPDIAPQPPGTISHIGDEGILVSVTFGNILV
                     TKVRSPSEGKLSAHELAAKKGWVVGDCFEQKP"
     gene            complement(2454..3515)
                     /locus_tag="PLM79_13080"
     CDS             complement(2454..3515)
                     /locus_tag="PLM79_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOV87290.1"
                     /translation="MEKKDQTLRIAAISLSLCVGAVLMVAKFYAYWLTQSSAILSDAL
                     ESIINVVASGFGLGSILLAAKPPDKTHPYGHGKIEYFSAGFEGALIVFAAIGIVRSGW
                     SQLWQPRELPALTGGLWILLGTMVVNLFLGLGLLRVGARTRSLALVADGKHVMTDVYT
                     SAGVLVGLAAVGYSGWYWMDGAIACLVAVNILYIGGKLIRESFAGLMDASDPALLEEI
                     SLFIAGHRKETWIDIHRLRAWRSGNRVHVDFHLILPRDLTLDQAHNEVTQLEEILRHH
                     LGRDAETLIHAEPCIDPECPICARDPCELRREETQRQPLWHQEVLTARPETEERPTTP
                     GVETEPGSQPSPKDSKPSP"
     gene            4006..6045
                     /gene="uvrB"
                     /locus_tag="PLM79_13085"
     CDS             4006..6045
                     /gene="uvrB"
                     /locus_tag="PLM79_13085"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOV87291.1"
                     /translation="MKPFALRADWEPTGDQPQAIAQLTEGLLAGTREQTLLGVTGSGK
                     TFTMAQVIANLQVPALVIAPNKTLAAQLYGEFKAFFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIAKDSSINETIDKMRHSATRSLLDRRDVIIVASVSCIYGLGSPESYREMLLQL
                     RTNTEVSREQVLHKLVEIQYTRNDEDFHRGVFRVRGDVLEIFPSYEEDRAIRVEFFGD
                     EVDTIKEVDPLTGRTLRYLESVAVYPGTHYVTSRDNLERAVGDIQRELAEVLDSFRRE
                     GKLLEAQRLEERTRLDIEMLLELGYCSGIENYSRHLDGRGAGTPPATLLDYFPDPWIL
                     FVDESHITIPQLRGMYRGDRSRKETLVRYGFRLPSALDNRPLSFEEFESKVRQVVYVS
                     ATPGPHETSRTRGHVVEQIIRPTGLVDPAIEVRPADYQVDNLVGEIRTRTASGHRVLV
                     TTLTKRMAEDLTEYLAELNIRVRYMHSDIDTLERIELVRDLRLGEFDVLVGINLLREG
                     LDIPEVSLVAVLDADNEGFLRSERSLIQTAGRAARNESGTVILYANQVTDSIRRAMEE
                     TDRRRAIQLAYNREHGIVPRSVQKEIPDILAEYREREPAAAFERVEEPRARYEAGSEA
                     SLEARISSLEKAMKDAASRLEFEKAAMLRDEIRHLRRRQLFAETPGETLQQMSGP"
     gene            complement(6024..6962)
                     /locus_tag="PLM79_13090"
     CDS             complement(6024..6962)
                     /locus_tag="PLM79_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOV87292.1"
                     /translation="MELFISGSLAYDRIMDFPGHFADHILPHKIHVLNVCFNINGLVE
                     KFGGTAGNIAYTLAMLKENPFIVATCGDDFERYARWLARNGMPTRWIKKIPGVLTAGA
                     YITTDLDDNQITAFNPGAMAYEADIPPLNSGRENALVFIAPGNKKDMLAFAEEARSAR
                     VPFFFDPGQSLNIWSGEELREAVKGALCFVSNDYELSLFLQMTGWNLDTLRKQCSVIL
                     TTRGPEGAVLDVAGDQTLIPALPVSGVLDPTGAGDAFRAGVLKGYTLGLDWQICCQMG
                     TVAASFVVECYGTQEHRLDWGTFCNRYEAHYGPLIC"
     gene            7220..7966
                     /locus_tag="PLM79_13095"
     CDS             7220..7966
                     /locus_tag="PLM79_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87293.1"
                     /translation="MRARPVRPFLYCLGVVLVLHWMADVHGVHAGALRITMQDGTSLE
                     VPYYWEEGGEIKFEIAGGVAGLPKGQVASVQEILSAREFDPEVLLQPPIPAAEVNEKQ
                     VLQELLEAKLPSSGTGQKVDPEEGTRLLRQAGAVRESRPAGERIYGPTINLEGDFIEL
                     VKSENEGLMLEMRKVISSRTELKSQQLVLVLYDGEGSVLQRKTCRVQPLDLDLQTRQK
                     LDLRGKLYLVTATLKPDQRIKRYEITAAQP"
     gene            complement(8249..9763)
                     /locus_tag="PLM79_13100"
     CDS             complement(8249..9763)
                     /locus_tag="PLM79_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87294.1"
                     /translation="MNAFLIYVRDENFYQLLPPKRTQSPPGNGRVRVMAMPPLGIQTL
                     APVLRLHGHGVRLFDTCHPQMKAEQIARAVIEDPPDVIGISFLSTTTYPAAKRMAGRL
                     RSANPRIPIIVGGAFASGNSDYILRDCPSIDCVGVGEGEELLPDYMNRLDDPGAVQGL
                     VWRDGRQIVRNPLRPLIRDLDQFPYPDRTSLPIDYIESLPLDVPAVLSLKKFCTMQTS
                     RGCPYSCIYCDIPSLTQGRWRSRSAAHVLGEMQELNDMGYRSVYLTDDHFLLKRKRIR
                     DICRGIVERKLEFTWGCEGRVDSLAVDQLADMSRANCTFLAFGVESGTQKILDRLNKR
                     QTLEQISKAVSTAKRNGIQRVHGFFVIGSPGETKGDIRASFRFAADLELDTFNFNRLC
                     AYRGTPLWREYVGEGILDDEQDWDKWFKCSDIDPTVLPGETLNRLRSRGYALLFAHRL
                     FRRPIQTLRLLKAFGRHMKIRDMLRLLLSPFRRRNLSRSPDLPAARQAAKPCSS"
     gene            complement(10133..11503)
                     /locus_tag="PLM79_13105"
     CDS             complement(10133..11503)
                     /locus_tag="PLM79_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOV87295.1"
                     /translation="MTENRSEEILAYYITSHGFGHAVRSFEIIRSLRRKAPGVRIVLV
                     SDLPEFLLEQVRDPGVFFRRKKADLGLVQLDSLRFDLEATFLSVREMYQNRGVLVDEE
                     LEFLEKTGVRLLVSDVGFLPLVAAARAGIPAVAVGNFTWHWIYESYAREDPRWAPLVE
                     WVGGCYRRCSLFLRLPMHGEDPEWSEGVEDVPLVARRARLSPNEVRKILDCPPDRKLY
                     LIAFGALDLSPEAQRRLEERKEALFLYRHPLRFDFANGRSVDSCEQLSYADLVGAVDA
                     VVTKPGYGIVSDCLAHGTPMIYTDRGFFIEYEVLVREIRRHLTWAYLSSDDLVAGNWS
                     PALEAIRKSPRKVPSIRDDGADVCARRILSLLNEETAVFPIEEVLDLHAFDPKEVPDL
                     LDDYLRAAFQAGMTRVRIIHGKGSGVLARRVQSLLSRHPLVTSSRSATPDSGGWGATV
                     AFLKET"
     gene            11642..>12344
                     /locus_tag="PLM79_13110"
     CDS             11642..>12344
                     /locus_tag="PLM79_13110"
                     /inference="COORDINATES: protein motif:HMM:NF036766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOV87296.1"
                     /translation="MSRIKVSFQPMDETVQADEGERVLDIASRAGIDISNLCGGEGVC
                     GRCRVQVRRGAVNFSTKHLAFLDKKDLEEGYVLACQSRVRGEDVEVRVPSESVIEEEQ
                     VLTFDRILAFETPTPLPRRAAPPKIPYFQPLCRKMYLELPQPTLSDSLSDLDRIYRAI
                     QKRMPGVHPDIHYTCLRGLGELIRENDWKITASVHLRDRDRPRIRAVEGGNTSSRNFG
                     VAVDVGTTTIVAKLVD"
BASE COUNT         2503 a         3755 c         3738 g         2348 t
ORIGIN      
        1 gaaggactgg tccgtcttca ggccccctgc aacgcccgga agattttcac ggtaagacca
       61 ccaatttttt gtctagacaa tgggggaaac cttagccggc actctggagt tccaggagga
      121 cggaaagtcc agaacactgg gtttcgtcat tgaggatggt cgtatcgaag attatgtcga
      181 cgccatgtaa gcatcatcct gcatcgttct tgcccgtcga gaatatctgg tgaatgccct
      241 gggcgaaaga acccttagac gcttccactt gattaagatc accaacctgt tcgattcaaa
      301 tgagggttag aattctttat ttctttatgc aaaaaacggt tccgaaaccc gcaaaatcgc
      361 cctaaagttc tttgcacata aaatgatcga attcaaaacc accgatcccg ttataaccac
      421 accaggtcca cgtttagctc acaaaagtct cactaaattg gctctagcaa ataatcattc
      481 tatatgctca cccccagcgg ttgttcgtcc tgcctgttgg ggatgcgggt ggggacggtc
      541 ccaaacgtcg acaaaaaagg gctcggattt tcacccgagc ccttgatttt cttggtagcg
      601 gggggaggat ttgaacctcc gaccttcggg ttatgagccc gacgagctac ctggctgctc
      661 caccccgcgt cagcaatgcg ccgtacctta ttcacattgc ctccctttgt caaggaagga
      721 tgctcgcgca tttgaaaaag taatcgatta taaccgcgct tggcgaacaa gccaagagcc
      781 ccggcaaaaa tccggggtct gaaacaagac ttccactcgg gcggcccaag aagccggttc
      841 ccttcttaga gggaaccttt ggacgacggg acctcggtgc gccgggggtc cagaccgacg
      901 gcccggtcga gggcccggcc ccaggccttg aacacggcct ccaggatgtg gtgggtattc
      961 cttccgcagg acacggtaat gtggagggtc acgcctccgt ggtggcaaaa agctcggaag
     1021 aattccggaa ccagctccgt ctcgaattcc cccacccggt cgggaagatc cggcacctcc
     1081 cagaccaggt acggacgatt ggaaaggtcc agcacgaccg tcgcccgggc ctcatccatg
     1141 ggcaccaccg cgctgccgta tcttcggatt cccttccgat cccccaaggc cgccgagagg
     1201 gcccggccca ggcagatccc catgtcttcc accgtgtggt gagcatccac ctccaggtct
     1261 ccccgggccc ggatcttcag atcgaaaaac ccgtgcacgg caaagagggt cagcatgtga
     1321 tccaggaaag gcaccccgga ttggatctcc gcaatccctt gcccgtccaa agaccattcc
     1381 gtttcgatcc gagtctcctt ggtttctcgt tcaatccggc ttttgcgctc attgctcatg
     1441 ggtcacccgt ctttctgcgt atcgagtccg tgttggattc tgctttgggg ggaggttcct
     1501 gtcgatcaag gtttctgttc gaagcaatcc cctacgaccc agcctttctt tgccgccagc
     1561 tcgtgagcgg agagcttccc ttccgagggg gaccggactt tcgtcaccag gatattcccg
     1621 aaggtaaccg acaccaggat cccctcgtcc ccgatgtggg aaatagtgcc cgggggttgg
     1681 ggggcaatgt cgggaaccag tttcgccccg aagaaccgga tcttttcccc cttcaggtag
     1741 gagtaggctc ccggctgggg gtcgcagcct cgaatccagt cgtagatttc caccgcggat
     1801 cggttccagt cgatcctcgc cacccgatcg tcgcaggggg gctcgtaggt ggccccttct
     1861 tccttctggg gaatccgggg ggcccgccct tccttgatca tctccacaga ttccacaatg
     1921 gcctggatcc ccatgggaaa caggtggttg aagtacaggg accccgtggt gtcctcgggc
     1981 cggatctcga tttccttctg gaggaggatg gggcccgtat cgattcctcc gtccggccaa
     2041 aagatggtca gacccgttcg ggtgtctccc atcatgaccg cccagttgat ggcgctcgcc
     2101 ccgcgatgcc ggggcagaat cgacggatgg taacagatgg ctccgtgggt tgccgcctcg
     2161 aagtaccttg ccggaatgat ggccgtgacg aaggccagga tcgccaggtc gggtttcaga
     2221 tcccggtagc gatcaaacac agcctcgtct cgataggcgg ccggctgaat cacctcgatg
     2281 ttccgggatg cggccaggga cttcagggga tcggctttgc cgcccggctt gtcgggaggt
     2341 gtgaagactc cgaccacgtt ctcacccttt tccagcaatg cctccagggt cttttcccca
     2401 aaagcggcct gaccgatgac cacaattctc atcttcccct ctcctttttt ctttcagggt
     2461 gacggtttcg agtccttggg ggacggttgg gaccccggtt ccgtttccac cccgggggtg
     2521 gttgggcgct cttccgtctc gggacgggcc gtcagcacct cttgatgcca aaggggctga
     2581 cgctgggtct cctcccggcg cagttcgcac gggtcccgtg cacagatggg acattcgggg
     2641 tcgatacagg gctccgcgtg gatgagtgtc tccgcgtctc ggcccaggtg atgccggagg
     2701 atctcttcga gctgagtcac ctcgttatgg gcctggtcca gggtgagatc ccggggaaga
     2761 atcaggtgaa aatccacgtg gacccgattg ccggaccgcc aggccctcag ccggtggata
     2821 tcgatccacg tttccttgcg atgcccggca atgaaaagag agatttcttc gagcaacgcc
     2881 gggtccgaag catccatgag cccggcgaag gattcccgga tcagttttcc tccgatgtag
     2941 aggatgttga cggccacaag gcacgcaatg gccccgtcca tccagtacca gcccgaatac
     3001 cccaccgccg ccaaccccac gagaacgccc gcggaggtgt acacatccgt cattacgtgc
     3061 tttccgtccg ccaccagggc cagggatcga gtcctcgccc ccacgcgcag caatcccagt
     3121 cccaggaaca ggttcaccac catggtgccc aagaggatcc acaagccgcc cgtcaaggcg
     3181 gggagttccc ggggttgcca cagctgactc catcccgacc gcacgatccc gatggcggca
     3241 aagacgatga gggcaccttc gaagcccgcg gaaaagtact cgatctttcc atgcccgtag
     3301 ggatgggtct tgtccggcgg cttggcggcc agcagaatgg aacccaatcc aaagccgctt
     3361 gccaccacgt tgatgatgga ctcgagggcg tccgatagga tggcggatga ctgcgtcagc
     3421 caataggcgt agaacttcgc gaccatgagg accgctccca cacagaggga caggctgatg
     3481 gcggcgattc gaagggtttg atcctttttt tccatgctct ccaccgggaa cgaagcgatt
     3541 gccgtgagac cctttcattc cattctaggg gaacacgttt ccctccacaa ggggaaggtc
     3601 ctttgaggat tcgggttcct ccaccccgcc aaatctcgcc gaaaagcccc cggtcgaggg
     3661 ggcggtcatc ggcgtccgct gcgccgggcg ggcgaccatt cgaggcgggt cccttctcga
     3721 cacgggcgac gggggcatct ctcgggtcga tgagcctctg cattggaaaa gggacgcgct
     3781 ccggttatag tggaaagaat acccgacgcc tgcttccccg ccgtaagccc ccagcgcccc
     3841 ggggagggta acgaaaacgc ctcctacgcc cgggaaagga gatgcaaagg ggcggcttca
     3901 acccgggggg aataaggcct cgtgcgggga aacgacccag gaatcgcctc ccggaaaagg
     3961 gagaccatcc ttcaacagct ttccaggggg gacttcggaa gacccatgaa accttttgcg
     4021 ctcagggcgg actgggagcc tacgggcgac cagccccaag ccatcgcaca actgaccgag
     4081 ggcctccttg ccgggacccg ggaacagacc ctcctggggg tcaccggttc gggaaagacc
     4141 tttaccatgg cacaggtcat tgcaaacctg caagtacccg ccctcgtcat cgcacccaac
     4201 aagaccctgg ccgctcaact ctacggcgag ttcaaggcct ttttcccgga aaatgccgtg
     4261 gagtactttg tctcctacta tgactactat cagcccgagg cctacgtacc ccaaacggac
     4321 acttacatcg ccaaggactc gtccatcaac gagaccatcg acaagatgcg ccattcggcc
     4381 acccggtctc ttttggatcg cagggacgtg atcatcgtag ccagcgtttc gtgcatttac
     4441 gggttgggat ctcccgaatc ctaccgggag atgcttctcc agcttcgtac caacacggaa
     4501 gtgtcccggg agcaggtcct gcacaagctc gtggagattc aatacacccg caacgacgag
     4561 gactttcatc gaggggtttt ccgggttcgg ggggacgttc tcgagatctt cccctcctac
     4621 gaagaggacc gggccatccg ggtggaattc ttcggcgacg aggtggacac catcaaggag
     4681 gtggatcccc tgacggggag gaccctccgt tacctcgaga gcgtcgccgt ctacccgggg
     4741 acccactacg tgaccagccg ggacaacctg gaacgggccg tgggcgacat ccagcgggaa
     4801 ctggccgagg tcctcgattc tttccgccgg gagggcaaac tgctggaagc ccagcgtttg
     4861 gaggaacgta cccggctgga catcgagatg ctcctggaac tgggctactg cagcggcatc
     4921 gagaattact cgcgacatct cgacgggcgc ggggcgggaa cgccccccgc caccctcctc
     4981 gattatttcc cggacccctg gatcctcttt gtggacgaga gccacatcac cattccccag
     5041 ttgcggggca tgtaccgcgg agaccgctcc cgcaaggaaa ccctggttcg ctacggtttt
     5101 cgactgcctt cggccttgga caaccgaccc ctgtccttcg aggaattcga atccaaggtg
     5161 cggcaggtgg tctacgtctc ggccactccc ggcccccacg agacgtccag gactcgaggc
     5221 cacgtggtgg agcagatcat ccgacctacg ggcctggtcg accccgccat cgaggtccga
     5281 cccgccgact accaggtgga caatcttgtg ggcgaaatcc gcacacggac ggcatccggc
     5341 cacagggtcc tggtgaccac cctcaccaag cgaatggccg aggacctgac ggaatacctg
     5401 gcggagctca acatccgggt ccgctacatg cattcggaca tcgataccct ggagcgcatc
     5461 gagctcgtgc gggatcttcg cctgggagaa ttcgacgtgc tggtgggaat caacctgctg
     5521 cgcgaggggc tcgacatccc ggaagtctct ctggtagccg ttctggatgc ggacaacgag
     5581 ggctttcttc gttcggagcg gtccctcatt cagacggcgg gacgggccgc gcgaaacgag
     5641 tccggcacgg tgatcctcta cgccaaccag gtaacggatt ccatccggcg ggccatggaa
     5701 gaaacggatc gccgacgggc catccagctg gcctataacc gggaacacgg cattgttccc
     5761 cggtcggtgc aaaaggaaat cccggacatt cttgcagagt atcgggagag ggaaccggcc
     5821 gccgcattcg agagggtgga ggaaccgcgg gcccgctatg aggcgggctc cgaggcatcc
     5881 ctcgaggcaa ggatttcaag cctcgagaag gccatgaaag atgcggcgtc ccggctcgag
     5941 ttcgagaaag cggccatgct ccgggatgag atccggcacc tgaggcgccg gcagttgttt
     6001 gcggaaacgc ccggggaaac ccttcagcaa atgagcgggc cgtaatgggc ctcgtagcga
     6061 ttgcaaaagg taccccagtc cagtcggtgt tcctgggtcc cgtagcactc gaccacgaaa
     6121 gaggcggcta cggttcccat ctggcagcaa atctgccagt ccaggcccag ggtgtatccc
     6181 ttgagaaccc ccgcacggaa cgcgtccccg gcgcccgtgg gatcgagaac gccggaaacg
     6241 ggcaaggccg gaatgagggt ctggtccccc gccacatcca gcaccgcccc ttcgggcccc
     6301 cgggtggtga gaatgacgga gcattgcttg cggagagtgt ccaggttcca gcccgtcatc
     6361 tggagaaaaa gggacagctc gtagtcgttg gatacgaagc acagggcccc cttcaccgcc
     6421 tcccgaagct cctcgccgct ccagatattc aggctctgac ccgggtcaaa gaagaagggc
     6481 acccgggcgg atcgagcctc ctcggcaaac gccagcatgt ccttcttgtt cccgggggcg
     6541 atgaacacca gggcgttctc cctgcccgaa ttgagagggg gaatatcggc ctcgtaggcc
     6601 atggcgccgg gattgaacgc ggtgatctgg ttgtcgtcca ggtccgtggt gatgtaggcc
     6661 cccgccgtga gcaccccggg aatctttttg atccaacggg tgggcatgcc gttgcgggcc
     6721 agccaccggg catagcgttc gaaatcatca ccgcaggtcg ccacgatgaa gggattttcc
     6781 ttgagcatgg ccagggtgta agcgatgttg ccggccgtcc cgccaaattt ttccaccagg
     6841 ccgttgatat tgaaacatac gttgagcaca tggatcttat ggggtagaat atgatccgca
     6901 aaatgtccgg gaaaatccat gatgcgatca tatgccaatg acccggagat gaatagttcc
     6961 atagacctag gtctccgttc ttcttgggga aagtctttct ctcatccgtg ttgcggcggg
     7021 cctacttccc gtatctgcgg gaatccggag gatgcgggag tttcgcccgg ggagtccgtc
     7081 cccctcgccc gtagccgtcc gggacacgga gaggaaacga gcccgtccgg ttcgatccga
     7141 tgccaaaccg ggctttttcg catggttctc tatactaaaa aacctacatg gggcaaagtc
     7201 aaaaaggatg aaaagaatca tgagagctcg acctgtgaga ccattccttt attgcctggg
     7261 ggtagttttg gtgttgcatt ggatggccga cgtccatggc gtccacgcag gagccctgag
     7321 aatcaccatg caggacggga cttccctcga ggttccctac tactgggagg agggcgggga
     7381 gatcaagttc gagattgccg ggggggtcgc cgggctcccc aaaggtcagg tggcatcggt
     7441 gcaggagatc ctctccgccc gggagttcga ccccgaagtg ctgctccaac cgcccatccc
     7501 cgccgccgaa gtgaatgaga agcaggtcct gcaggaactg ctcgaggcca agcttccttc
     7561 ctccgggacc ggtcagaagg tggaccccga ggaaggaacc cggttattgc gtcaagcggg
     7621 agccgtccgg gagagtcgtc ccgccggtga acggatctac ggcccgacca tcaatctcga
     7681 aggggatttc atcgaactgg tgaagtccga aaacgagggt ttgatgctgg agatgcgcaa
     7741 ggtcatcagc agtcggacgg aattgaagag tcagcagctt gtgctggtgc tctacgacgg
     7801 ggagggcagc gttctccaga ggaagacctg ccgggttcag cccctggacc tggaccttca
     7861 aacccggcag aaactcgacc tgcgaggaaa gctctacctc gtcacggcca ccctcaagcc
     7921 cgaccagagg atcaagcgat acgagatcac ggcggcccaa ccctagcagg accttttacc
     7981 tacatctttg ctattggacg agataccagg ggtcaggtct ccgtttggct tgaccatagg
     8041 ggtcacatct ccgtatggtc cgtatggctt gtcccgtagg tggaaagggg gcaggggcga
     8101 ccggccgctc gcccctacca ggcgtgcgag gcgcaatccc gctcttgtcc gcgggacggg
     8161 ccgcccttct ttccatcctc ttcggaaggc cccggggggg tagatgcatt gcccgcctgc
     8221 agggaacccc tttcgaccct cgacggattc aggaagaaca gggtttggcc gcctgcctcg
     8281 cggcgggaag atcggggctc cgactcaggt ttctccgtcg aaaggggctc aagagcaatc
     8341 tcagcatatc ccgaatcttc atgtgacggc cgaaggcctt caggagacga agggtttgga
     8401 tcgggcgccg gaagaggcgg tgggcaaaaa gcagggcata gccccggctg cgcagccggt
     8461 tgagggtttc gccgggcagg accgtgggat cgatgtccga acacttgaac catttatccc
     8521 aatcctgctc gtcgtcaagg attccctccc cgacgtattc ccgccataaa ggggtccccc
     8581 ggtaggcgca caagcgattg aaattgaagg tatccagttc gaggtccgcc gcgaaacgaa
     8641 aactggcccg gatgtcccct ttcgtttctc ccggagagcc tatgacgaag aacccgtgca
     8701 ccctttggat cccgttccgc ttggccgtgc tcactgcctt ggaaatctgt tcgagggtct
     8761 ggcgcttgtt cagccggtcc aggatcttct gggtcccgga ctcgaccccg aacgccagga
     8821 aggtgcagtt ggcccggctc atgtcggcca actgatccac ggccagggaa tccacccgtc
     8881 cttcgcaacc ccaggtgaat tccagctttc gttccacgat cccccggcag atgtcccgga
     8941 tgcgcttgcg cttgaggaga aaatggtcgt cggtgagata aacggagcga tagcccatgt
     9001 cgttcagttc ctgcatttct cccagcacgt gtgccgcgga ccgactgcgc catctccctt
     9061 gggtgagaga agggatatcg cagtagatgc acgagtaggg gcagccccgg gaagtctgca
     9121 tggtgcagaa ttttttcagg gacaggacgg cgggaacgtc caggggcaac gattcgatgt
     9181 agtcgatggg aagactggtc cggtccggat aagggaactg atccaggtcc cgtatgaggg
     9241 gtcgcagcgg gttgcgaacg atttgcctgc cgtctcgcca caccaggccc tgcaccgccc
     9301 ccggatcgtc gagacggttc atatagtccg gcagaagctc ttctccctct cccaccccga
     9361 cgcagtcgat ggaagggcag tcccgcagga tatagtccga attcccgctg gcgaaggctc
     9421 cacccacgat gatgggaatc cgggggttcg ctgatcgaag gcgaccggcc attctcttgg
     9481 ccgcagggta cgtggtcgtg gaaaggaacg aaatcccgat gacgtcgggc ggatcttcga
     9541 tcacggcccg ggcgatctgt tccgccttca tttggggatg gcaggtgtcg aagagcctca
     9601 ccccgtgtcc atgcaggcgc aagaccggag ccagggtctg gatccccagg gggggcatgg
     9661 ccatcacccg gactcggccg ttgccgggcg gggactgggt ccgtttgggc ggcagcagtt
     9721 ggtagaaatt ctcgtctctt acgtagatca ggaaggcatt catcaaaaaa ttctcccgtt
     9781 cgggagactc tccggcgcct tccccccgga gggacggagc cggatgcaag tctcgttaaa
     9841 ataccggcgc acaggattcc gttccctccg catcgggtag gacttacggg ccgaataatt
     9901 ctcgatcagg ccgggggctg agggctgaga aggtaaccga ttggtgggga caattcaagg
     9961 gcagtcccga ttgcccctga aaagagtttt tccgggcacc gaagcccgga agggttcggg
    10021 attggaccaa ggccgtcccg gccaaggcac cgggcatggg acttcccggg gaggcgggcg
    10081 gctctttcgc cgggggacgc ttcttccggg cgcggccgtc gcgactccgc cctcaggttt
    10141 ctttcaggaa cgccacggtc gctccccagc cgcccgaatc cggggtcgca gaccgagaag
    10201 aggtcaccag gggatgcctc gagagaaggc tttggactcg acgggccaaa actccgctgc
    10261 ccttgccgtg gatgatgcgc actcgggtca ttcccgcctg gaaagcggcc ctcaggtaat
    10321 cgtccaggag atccgggacc tctttggggt caaacgcatg caaatccagg acctcttcga
    10381 tgggaaaaac ggccgtctcc tcgttcagga gggaaaggat acgccgggcg cagacatcgg
    10441 ccccgtcgtc ccggatggaa ggaaccttcc tgggactttt ccggatggct tccagggccg
    10501 gggaccagtt gcccgccacc aggtcgtcgg aggacaggta agcccaggtc aggtggcgcc
    10561 ggatctcacg gaccaggacc tcgtactcga tgaagaagcc gcggtccgtg tagatcatgg
    10621 gggtcccgtg ggccaggcaa tcggacacga tgccgtaccc tggcttggtg acgacggcat
    10681 ccaccgcccc cacgagatcc gcgtaggaaa gttgctcgca ggaatccacg gaccgaccgt
    10741 tggcaaaatc gaagcgaaga ggatgtctgt acagaaacaa ggcttccttc ctctcttcga
    10801 gcctgcgctg ggcttccggg gacaggtcca gggccccaaa ggcaatgagg tagagttttc
    10861 ggtcgggggg acagtccagg atttttcgaa cctcgttggg actgagccgg gctcggcgcg
    10921 ccaccagggg cacatcctcc accccttcgg accactcggg atcctccccg tgcataggaa
    10981 gtctcagaaa caggctgcac cggcgatagc acccgccgac ccactccacc aggggagccc
    11041 atcgaggatc ctctcgggcg taggattcat aaatccagtg ccaggtgaag ttcccgacgg
    11101 ccacggcggg gattcccgcc cgggccgcag ccaccagggg caggaacccg acatcggata
    11161 ccaggagccg aacgcccgtc ttttcgagga actccagttc ctcatcgacg aggacccccc
    11221 ggttctgata catttcccgg acactcagga aggtggcctc gaggtcgaat cgtaagctgt
    11281 ccagctggac cagccccagg tccgccttct ttcttcgaaa gaagaccccc gggtcccgga
    11341 cttgttccag caggaattcg ggaagatcgc tcaccaggac gattcggacc cccggagcct
    11401 tcctgcgcag ggagcggatg atttcaaagg accgaaccgc gtggccaaac ccgtgggagg
    11461 tgatgtaata agcaaggatc tcttcggaac ggttttcggt catggagctt ctcgtcgggg
    11521 tgaggtccgt tgaaagacgg ggagaggtcg acccgggccg attttgggac ggcgtgagaa
    11581 tagcggttcc ctctcgggga gtcaatcccg tgaaaggtcc ggaaaccagg gaggtgaatt
    11641 catgagcagg atcaaggtat ccttccaacc catggatgag acggtgcagg ccgacgaggg
    11701 ggagagggtc ctcgacatcg cctcccgcgc cgggattgac atcagcaacc tgtgcggagg
    11761 cgaaggcgtc tgcggtcgat gccgggtgca ggtccggcgg ggtgccgtca atttttccac
    11821 caagcacctg gccttcctgg acaaaaaaga cctggaagaa ggctacgtat tggcttgcca
    11881 gtcccgggtc cgcggggaag acgtcgaggt gcgggtccct tccgagtccg tcatcgagga
    11941 agagcaggtc ctcaccttcg atcgaatcct ggccttcgaa acccccacgc ctttgcccag
    12001 gagggccgcc ccccccaaga ttccctattt ccagccctta tgccgaaaga tgtatctcga
    12061 gcttccccaa cccaccctgt ccgacagcct gtcggacctg gaccgcatct atcgggccat
    12121 ccagaaaaga atgccggggg tccatccgga catccactac acgtgtttgc ggggcctggg
    12181 agaattgatc cgggaaaacg actggaaaat cactgcctcc gtccacctga gggatcgaga
    12241 ccgtccccgt atccgcgccg tcgagggagg caacaccagc agccgaaact tcggggtggc
    12301 cgtggatgtg ggcaccacca ccatcgtggc caagctggtg gacc
//
LOCUS       DAOOVC010000113        12234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3389_length_12234_cov_6.646687, whole genome shotgun sequence.
ACCESSION   DAOOVC010000113 DAOOVC010000000
VERSION     DAOOVC010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_12234_cov_6.646687"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..832
                     /locus_tag="PLM79_13115"
     CDS             212..832
                     /locus_tag="PLM79_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87297.1"
                     /translation="MASKGKKPDLESHHKKYLAPIIKQFPFLLRITEWKDYPAPVLVV
                     KERPLSAGDNSPRRKSAEPDSGSKSVEIGFIAGEPQRLCLPIFRQIVERVRDRGGVPL
                     DLQRFLSIEGLRLRMNLPLDEEAGSKLGLIFRLQLRVKEFDRIELMARRIARFTAEEA
                     SYWLSRTTHFGPDANRWAVSGMRIMLGGQPKDPAVERMLEKLRTGS"
     gene            902..1036
                     /locus_tag="PLM79_13120"
     CDS             902..1036
                     /locus_tag="PLM79_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87298.1"
                     /translation="MNEREPEMTNKEIAIKTIEQLPPDATWEDIQERISFVAGARRGL
                     "
     gene            1221..1457
                     /locus_tag="PLM79_13125"
     CDS             1221..1457
                     /locus_tag="PLM79_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOV87299.1"
                     /translation="MDMPTRGSRVLEEALSLPPAERAEIAERLLSSLDPPTQAALDRL
                     WAEEAEDRIDAFEGGEITAIPASRVFHHLPKNIK"
     gene            1454..1759
                     /locus_tag="PLM79_13130"
     CDS             1454..1759
                     /locus_tag="PLM79_13130"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOV87300.1"
                     /translation="MKVLFLEAAEQELIEAEEYYNGQREGLGFEFSEEVRATINRIIE
                     YPDAWSPISRRTRRCRTKRFPYGIIYQVMEEVLLIVAVMHLQREPESWRRRVSKRLR"
     gene            complement(2061..2747)
                     /locus_tag="PLM79_13135"
     CDS             complement(2061..2747)
                     /locus_tag="PLM79_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87301.1"
                     /translation="MKLIYHFPPELLPPILNIISQLRAKSSWEIECCIQDDRTNWIEF
                     ICDNTMPQSQGLKRNGTTSCRRIKHNHVTWFRLNGVCICPSIPILVFWSPNPLRCLPR
                     NPKLRPRRIPIDALNAEATAGFGPWERNRQALLLPTLQHRIVRQTRILGPDAGNQPEI
                     LVTPVLFRLQRAKHERVHGQELTAVFALLNAGEEISREGPRHAVDDGPDPFYAELAHK
                     GVGVRVRRTQ"
     gene            2850..5126
                     /locus_tag="PLM79_13140"
     CDS             2850..5126
                     /locus_tag="PLM79_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87302.1"
                     /translation="MDGFIYTRQVTCPNCGGEAPLLNTCWLSKEAGDLWGVKVVTDGK
                     PRNGKVYFQTYRVTKGKGPGGEDPNFATVDRGVGRCVHCKQAITGEEIKAQARGESPR
                     GRWKDRLYAVVAVRLEPKLDKHGKPQRFKSGPKAGEIKTQKVRFFRPPNELDLRALQM
                     AEERLKAKWDEWEAAGLIPTEWIPDGNDMRPVLYGMPRWCDLFTPRQLLGHLTLVEGL
                     NRLKPRIIAELGLDRARAVVTYLQFAIDKGLDYNSKQTRWEYTRGIVKGTFGRHDFSL
                     KWTFGEMIFTGPNSGAAWGLSQVIDAYRGIAELVEPVHAHLENPSPLSVTILNQTAAH
                     MPQIEDKTVDLVCMDPPYYNNVQYAELSDYFYVWQKRTLKDLYPDIFHSRLTNKREEA
                     VANPVRDGSAKAAKKAYEKMMGEIFSECRRVLKDEGLLTLMFTHKQQEAWETLTRSLI
                     EAGWIITASFPVESEASASLHQRDMAAAASSIFITCRKRERAHNDPAMWTGLGGRGVQ
                     RRITDAVQNALEEFVPLNLNPVDEMVACYGRALHVLSQNWPVMDGDEPVGPMRAMLEA
                     SRVVAENQVRRITGGRVKVGDLDTETGMALTLFGIYGLNAFPYDEALSLSRSLNIALT
                     NKPAGYRVEGRMIGINQEACGGTARSRGAGEQEVGYHAPLVRSGSKLRLARPEERDER
                     RMEQPQTDWDVLHGLIRAYRRGDAPVAQGYLNRHAGENPAKVLDLLDVWAAEVSEERL
                     RKEAEAMRFDLNLRAGNL"
     gene            5126..9004
                     /locus_tag="PLM79_13145"
     CDS             5126..9004
                     /locus_tag="PLM79_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV87303.1"
                     /translation="MRAGGAESATFKDFPWRISYRTSTLKPDGTSVDILHDFYIPALQ
                     RAVSYDRVAGFFRSTSLAAAAQGFSAFVGREGKARFVVGADLDPEDVLAILEGGRERF
                     EAALNRELGEPSKWPEKVVNGVQLLGWMVAHGHLEIKVAFRVHRESGQPIRFESVEDG
                     YVHMKWAVFSDERGNRIYISGSLNESKSALVLNAENIDVHCDWRGETDRLRAIEAVQE
                     FDLLWEDGNPAFKVLSLPEAVRQRLVEISQAVKRPLEIDGTSVAPVEVSPPSAMELLK
                     FALLRDGPRLPGGKFVGMETTPIVPWPHQSVVARRLIASWPASFLLCDEVGLGKTIEA
                     GLAIRSLVLSGLARRVLIAAPASLTRQWQREMAAKFHLPFRRALGGRPVRHEVLLPYE
                     RELPSESLFAPDLTIVSTGLLTRDDRQSDLKGAAPFDIALLDEAHCARRKNPNEGTRA
                     NPRYGNIYRIIDGELSRKAENLLLATATPMQMHPVEVSDLLKLTRRVGSFQLDPSLTM
                     IFYDVLGDVVSGKDISEREWEFLRRVVLSIRMLDPEHWKFIEKSVLDGRTRIPARHWL
                     EYGRVPKGTDRRRMLRLIFSAAPLSRAMLRHTRALLEVYRNRGRLDGMLAERQVLPLS
                     GITFTDQERVVYEQLETYCRELARQMSGKKDARGRSAIGFYLSFLRLRFASSLYAIRE
                     TVRRRMERVEATLQFHEGAALDDPEGWDAEDLLEEGDDDSEVVKTVLKDRTPEDLWWE
                     REHLKGMLKNLHDLSGQSSKMTELLKILQHRLVPGGDRIRQTVVFTRFFDTLTDIVSR
                     LRGANPRLLIGTYSGSGGQYLNPATLSLVGTERDIIKHKFLRGEIDVLVCTDAAAEGL
                     NLQTADFLVNFDLPWNPMKVEQRIGRIDRIGQRHKTIYVSNLCYVGSAEEIVYGRLWK
                     RLTDAGAVVGTQQISLLPVTTEEFHELAENPAFAKKLEKVAKERAILFQKHTASREIP
                     PDELFEIYERIEQDESARKVPVGLDDIWEALSNSTYLRELGCAPVFHAEQRVLKVSNI
                     PGVSQDSAVTTSRTTYEVGVTDLEERLHFATYGDAVFDAILGHVGEFELPGCLCRLET
                     RVPGLETEMIAYAAASIDEHGNPACRLLTSYQDLNGLYLDETRTLTDDDVKSALEALQ
                     RQAVEDFRTHLAVPRLKAENEVCANSQLILDYLTISGLIRERQFLGKSETLFWREIDA
                     IHEEYKDRENLRIRKIPAKIASRLSGLLFDVIIPQMGEEAFIDASQPLLKAAIDAICR
                     VADAMKVKKSELTTDDVLARVDRLVQQGFV"
     gene            9222..9533
                     /locus_tag="PLM79_13150"
     CDS             9222..9533
                     /locus_tag="PLM79_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87304.1"
                     /translation="MRNSRLDNGNLDKARRLLWPIKRKCGGKISWADPVILAGKVAPE
                     SMGFKTFGFGGGREDVRKQPRIGSRLEEQLRHRQRRRYHYQRPRGHMDRHADEAGRIE
                     P"
     gene            9789..10394
                     /locus_tag="PLM79_13155"
     CDS             9789..10394
                     /locus_tag="PLM79_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOV87305.1"
                     /translation="MRRRVIPSLTAAVLLLLPCAGLADRYQIDPAHTSLTFSVRHFGI
                     SNVKGHFDEFSGSIVLENGAIREASGTIQVNSINTGVRQRDDHLRTADFFDAPNYPQI
                     LFKTGRVEKSGEETVLVGDFTMRGVTRELRLPVRLNGPIKDPKGNLRIGLEAKTGLNR
                     KDYGIRYHAALETGVPLVGDEVAIEINAEAIKEVPGETGTQ"
     gene            complement(10653..>12234)
                     /locus_tag="PLM79_13160"
     CDS             complement(10653..>12234)
                     /locus_tag="PLM79_13160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87306.1"
                     /translation="SEQDEGSVVLEFNVRDTGVGIPNERLAYIFDAFSQADGSMTRKF
                     GGTGLGLAISKQLCEMMGGRISVVSEVGRGSTFSFTAKLKKQPSARRPQEEAVRDLKN
                     IRVLIVDDNAVSRKILHQQVVSWGMANGCAEDGAQALEKMRRAYERGEAYDVVILDRT
                     MPGMSGLELARAIRSDPDLSGARIILMTSVGEIEEMERTREAGVLACVTKPVRQSQLL
                     DAIVSVLSVPSARATEPREVEIPSTPGPAGLSGHVLLAEDNPVNQEVARGMLESLGCT
                     VVVVNDGREALEAVSRGSYDAVLMDCQMPEMDGYEATRLLRQSGRPEIPVIALTAHAM
                     EGDREKCLAAGMNDYLSKPFTREELGRILEQWLPKRFPGRSPASGPSSGSSPAGEDMD
                     SRPEPPTSTTSQDREGDAPDGDFIDWEVWNALRELEQKDDPDFLTMLIGLYVENTPSL
                     VRDLREAAASDDWNGLRRAAHSLKSSSANLGAKIPAALCRQLEALGQAGSTRDTSSII
                     ARLEKEIARFVDAAQRSL"
BASE COUNT         2577 a         3581 c         3747 g         2329 t
ORIGIN      
        1 cggaggtttt tcttgaaatc caagagcctg atgaagactg ccttttcatc caggcggtcc
       61 ggaaaaaata ggacggaacc ttcatgagcc cctgaaacaa tcgtgttgaa tcggattgaa
      121 acggcctgtc tgccaccccc ttggaccggg gggggcactc caaaccggca accgctgaga
      181 aacctttcaa ccggaaaggg aatttgggaa tatggcatcc aaagggaaga aaccggatct
      241 ggaatctcac cacaagaagt acctggcacc gatcatcaag cagttcccct ttctgcttcg
      301 gattaccgaa tggaaagact atccggctcc cgtgctcgtg gtcaaggaaa ggcccctttc
      361 agcgggggat aactccccac ggagaaagtc agcagaaccg gactcaggct ccaagtcggt
      421 ggaaatcggg ttcattgcgg gggaacccca gagactctgc ctccccatct ttcgacagat
      481 tgtggagcgg gtcagggaca gaggtggagt gcctctggat ctgcagcgct ttctctccat
      541 cgagggtttg agacttcgaa tgaacctgcc cctggacgag gaggccggct ccaagctcgg
      601 tctcatcttt cgcctgcaac tgcgagtgaa ggagttcgac cgcatcgaac tcatggcgcg
      661 ccgcattgcc cgattcaccg cggaggaagc gtcgtactgg ctttcacgca ccacccattt
      721 cggacccgat gccaaccggt gggccgtctc cggaatgcgc atcatgcttg gggggcaacc
      781 caaggacccc gcggtggaga ggatgctcga gaagctgcgg acagggagtt gaacgcgaag
      841 gtcccgacct ccctaatccc cacagccgtc ggggcatagg ataaaatgaa cgagaaagat
      901 catgaacgag agagaacctg aaatgacaaa caaggaaatc gccataaaga ccattgaaca
      961 attgccgccg gatgcgacgt gggaggacat ccaggaacgc atcagcttcg tcgcgggtgc
     1021 gcgccggggg ctttgagagc tcgacgaagg caggggaatt tcccatgaca aggtgaaacg
     1081 gaagccggcc gaatggcttt cgggtcaata gtgggtagtt caatgcttga cgaggatatc
     1141 tcgaaaggcg tcaaaggccg tcatggttgt tttctcgttt ccacgttctc acgtgggaac
     1201 tgccaaagca aaagggggta atggacatgc cgacaagggg ttcgcgggtg cttgaagagg
     1261 ctctttccct tcctccggct gagcgggccg agatagccga gcgtctgctt tccagcctcg
     1321 atccgccgac acaggccgca ctcgataggt tgtgggctga ggaagccgaa gaccgcatcg
     1381 atgctttcga gggtggggag atcacggcaa ttcccgccag ccgggtcttt caccatctgc
     1441 caaagaacat caaatgaaag tcctctttct cgaagcggcc gagcaggagc tcatcgaggc
     1501 agaggagtac tataacggtc aaagggaagg gctcgggttc gagttctccg aagaggtccg
     1561 ggcgacgatc aacaggatca tcgagtatcc ggatgcctgg agtcccatct ccaggagaac
     1621 caggcgctgc cgtaccaagc gcttccctta cggaatcatc taccaggtaa tggaggaagt
     1681 cctcctgatc gttgcggtca tgcacctgca acgtgagccc gagtcctgga gaagacgagt
     1741 ttccaaacgc ctccggtgaa tcccggaagt tcccttcgcg gacgctgaca tttgcctcgc
     1801 gaccatgctc atttctcgcc tgcctggagg gtacgatcga gccttgaacc ggagccgccc
     1861 gaatcacggc tccgcagttg aatggagtac acttggaccc gaaagatcaa cggctcatcg
     1921 agcgcggatt tccctgtcat caagttggag cggagactca acgggagcgc ggtgccagtt
     1981 cagcactacc gcctctttat ttcctgcatg tctggtgggc caggcgcccg ctgacccctt
     2041 ccagagcggc catcctggcg tcactgagtc ctgcggacac ggaccccgac acctttgtgc
     2101 gccaactcgg catagaacgg gtccgggccg tcgtcaacgg cgtgccgtgg accctcacgg
     2161 gagatctcct cgcccgcgtt gagcaaggca aagacggcag tgagttcctg cccgtggacc
     2221 cgttcgtgct tcgcgcgctg caggcggaac aggaccggcg tgacgagaat ctccggttga
     2281 ttgccggcat cagggccaag gatccgagtc tggcgaacga tccggtgttg aagcgttggg
     2341 aggaggagtg cttgcctgtt cctctcccat ggcccgaagc cggcggttgc ctccgcgttg
     2401 agcgcgtcaa tggggatccg gcgtgggcga agcttcggat tgcgtgggag acagcgcagg
     2461 ggattcggac tccagaagac aagtatgggt atggacgggc atatgcaaac cccgttaagc
     2521 ctaaaccaag tgacatggtt gtgcttgatc ctacggcagg aggtggttcc attccttttg
     2581 aggccttgag actggggcat agtgttatcg caaatgaact caatccagtt ggtgcgatca
     2641 tcttgtatgc aacactcgat ctcccaactc gatttggccc ggagctggct gatgatattg
     2701 agaattgggg gaagcagctc tggagggaaa tggtagatca gcttcatgat cttttccctg
     2761 catcgccgcc ggctggtgaa gaaaaggccc gtcttgccca ccatctaagc caatgcccag
     2821 agcttatccc acaatatagc agtgagagct tggatggctt catttacacc cgccaggtga
     2881 catgccccaa ctgcggaggg gaagcaccgt tgctgaatac ctgctggctt tccaaggagg
     2941 ctggtgacct ttggggcgtg aaggtggtca ctgatggaaa gcctcgaaac ggaaaggtct
     3001 actttcagac ctaccgagtg accaaaggga agggccccgg tggagaagac ccgaatttcg
     3061 caaccgtcga tcgtggggtt ggccggtgtg tccactgcaa gcaggccata accggcgagg
     3121 agatcaaggc tcaggcacgg ggagaatcgc cccgaggccg atggaaagat cgcctctacg
     3181 ccgtggttgc cgtgcggttg gagcccaaac tcgacaagca cggaaagccg cagcggttca
     3241 agagcggacc caaggctgga gagatcaaga cccagaaggt tcgcttcttc cgtccaccca
     3301 atgagctgga cctcagggcc ctccagatgg cggaggaacg tctcaaggcc aaatgggacg
     3361 agtgggaagc cgcgggtctc attcccacgg aatggatacc ggacgggaac gacatgcgtc
     3421 cagtcttata cggcatgcca cgttggtgcg atctcttcac tccacggcaa ctcctcgggc
     3481 acctcacgct cgtggaaggc ctcaaccggc tgaaaccgcg gatcatcgcc gaactcggct
     3541 tggatcgggc ccgggcggtg gtgacctacc tccagtttgc catcgataaa gggctcgact
     3601 acaacagcaa acagacgcga tgggaataca ccagggggat tgttaagggc acctttggcc
     3661 gccacgactt ttccctcaag tggacctttg gagagatgat tttcaccggg ccaaactcag
     3721 gggccgcatg gggattgtca caggtgattg atgcttaccg aggtatcgcc gagctcgttg
     3781 agcccgtaca tgctcatttg gaaaaccctt cgccactttc tgtgaccatt ctcaaccaaa
     3841 ccgcggccca catgccgcaa atcgaagaca agaccgtgga cctggtctgt atggaccccc
     3901 cctactacaa caatgtccag tacgccgagc tttccgacta cttctacgtc tggcagaagc
     3961 gcaccctcaa ggacctgtac ccggacatct tccacagccg gctcaccaac aagagggaag
     4021 aggcggtggc gaaccctgta agggacggtt cggcaaaggc agccaagaaa gcctacgaaa
     4081 agatgatggg ggagatcttt tccgagtgcc ggcgtgtgct caaggatgag gggctgctga
     4141 ccctcatgtt cactcacaaa caacaggagg cctgggagac actcacccgg tctctcatcg
     4201 aggccggatg gatcatcacg gcttcctttc cggtagagtc cgaagcttct gcatcactcc
     4261 accagagaga catggccgct gcggcaagct ccattttcat cacgtgccgc aagagagaaa
     4321 gggcgcacaa cgacccggcc atgtggaccg ggcttggcgg caggggcgtg cagcgccgga
     4381 tcaccgacgc cgtgcaaaat gcccttgagg agtttgtgcc tctcaacctc aatcccgtgg
     4441 atgaaatggt cgcgtgctac ggcagggccc ttcatgtgtt gtcccaaaac tggccggtca
     4501 tggacgggga tgagccggtc ggccccatgc gggccatgct cgaggcgagc cgggtggtgg
     4561 ccgaaaacca ggtgaggcgc atcacggggg gacgcgtcaa ggtgggcgat ctggacacgg
     4621 aaacgggaat ggccctgacc cttttcggca tctacggctt gaatgctttc ccttacgacg
     4681 aggcattgag cctctcccgc tccctcaaca tcgccctgac caacaagccc gccggctacc
     4741 gggtggaggg tcggatgatc gggatcaacc aggaggcctg cggaggtacg gcaagaagcc
     4801 ggggagccgg ggaacaggag gtgggatacc acgctcccct ggttcggtcg ggttccaagc
     4861 tccgactggc caggcccgaa gaacgcgatg aacgaagaat ggaacagccg cagacggact
     4921 gggacgtgct ccatggcctc atccgggcgt acagaagggg ggatgctccc gtggcgcaag
     4981 gttacctcaa ccggcacgcc ggggagaatc ccgccaaggt cctggacctc ctcgacgttt
     5041 gggctgcgga agtctccgaa gagagactca ggaaagaggc cgaggctatg cgcttcgatc
     5101 tcaatctcag ggccgggaat ctctaatgcg tgccggcgga gcggaatccg caacctttaa
     5161 ggacttcccc tggcgcattt cctatcggac ttccactctg aagcccgatg gaacctccgt
     5221 ggacatcctc cacgacttct acatccctgc gttgcagcgt gcggtgagct acgacagggt
     5281 ggccgggttc ttccgatcca cttcgcttgc cgcggcagcc caggggtttt cggccttcgt
     5341 gggcagggaa ggcaaggcgc ggtttgtcgt tggggccgat ctcgatcccg aggatgtcct
     5401 ggctatcctg gaaggaggcc gggagcggtt cgaggccgcc ttgaacagag agttgggcga
     5461 gccgtccaag tggccggaaa aggtcgtcaa tggagtccag ctgctcggct ggatggtggc
     5521 tcacgggcac ctggagatca aggtcgcctt tcgtgttcac cgggagagcg ggcaacccat
     5581 ccggttcgag tccgtcgagg acgggtatgt ccacatgaag tgggcggtct tttcggacga
     5641 acggggaaac aggatctaca tttccggatc cctcaacgaa tccaaatcag ccctggtgct
     5701 caatgcggaa aacattgacg ttcactgcga ctggcggggt gagacggacc ggctcagagc
     5761 cattgaagcg gtccaggagt tcgatctgct ttgggaggac gggaatcccg ccttcaaagt
     5821 cctgagcctt cccgaagccg taaggcagcg cctcgtggaa atctcccaag ccgtgaaacg
     5881 gcctttggag atagacggaa ccagcgtggc accggtggag gtgtcccctc cctctgccat
     5941 ggaacttctc aagttcgccc tgctgaggga cgggcccagg cttcccgggg gcaagttcgt
     6001 gggcatggag accacgccca tcgttccatg gccccaccag tccgttgttg caagaaggct
     6061 catcgcctcc tggcctgcga gtttcctgct gtgcgacgag gtgggtctcg ggaagaccat
     6121 cgaagcgggt ctcgccatcc ggtcccttgt cctctcgggg ctcgccaggc gggttctcat
     6181 tgcggcgccg gccagcctca cccggcagtg gcaaagagaa atggcggcca agttccatct
     6241 cccgttccgg agggccctcg ggggaaggcc ggtccgccac gaagtcctcc tcccctatga
     6301 gcgggagctg ccttccgagt ccctcttcgc cccggatctc accattgttt ccacgggcct
     6361 gctcacaagg gacgatcggc aatcggacct caagggggct gcgcccttcg acattgcgct
     6421 cctggacgaa gcccattgcg cacgaaggaa gaaccccaat gagggaacca gggcgaaccc
     6481 ccgatacgga aacatctatc gcatcattga cggtgagttg agccgaaagg ccgaaaatct
     6541 gcttctggcc acggcgactc ccatgcagat gcaccccgtg gaagtgagcg atctcttgaa
     6601 gctcacccgc cgtgttgggt cgttccagct cgaccccagc ctcaccatga tcttttacga
     6661 tgtgcttggc gacgtggtga gcggcaagga catctcggag agggagtggg aattcctccg
     6721 gagagtggtc ctgtccatcc ggatgctgga ccctgagcac tggaagttca tcgagaaatc
     6781 cgttttggac gggcgcacca gaattccggc gcgccactgg ctggaatacg ggcgagttcc
     6841 caagggaacg gaccgcagga ggatgctgcg cctcatcttt tcggccgccc ccctctccag
     6901 ggccatgctc aggcataccc gcgccctgct cgaggtctac cgcaacaggg gccggctgga
     6961 cgggatgctc gccgaaaggc aggtcctgcc attgtccggg atcaccttca cggatcagga
     7021 acgggtggtc tacgagcagc tcgagaccta ctgccgggaa ctcgcccggc agatgtccgg
     7081 gaagaaggat gcacgagggc ggagcgccat cgggttttat ctcagtttcc ttcggctgcg
     7141 atttgcctcg agtctgtacg cgatccgaga gacggtccgc cgccgcatgg agcgggtcga
     7201 ggcaaccctg cagttccacg aaggggcagc cctggacgat ccggagggtt gggatgcgga
     7261 agaccttctt gaggaagggg atgacgactc ggaggtggta aagacggtcc tcaaggacag
     7321 gacccccgag gatctttggt gggagcggga acatctcaag ggcatgctca aaaaccttca
     7381 cgacctctcc gggcagtcct ccaagatgac ggagcttctc aagatccttc agcaccggct
     7441 tgttcccggc ggagacagaa tcaggcagac ggtcgttttc accaggttct tcgatacgct
     7501 cacagacatt gtgtcgcggt tgcggggggc aaacccccgc ctgctgatcg gcacctactc
     7561 gggaagcggc gggcagtatc tcaacccggc aacgctttcc ctggtgggga ctgagcggga
     7621 cattatcaaa cacaagttcc ttcgcgggga gatcgacgtg ctggtgtgca cggacgccgc
     7681 cgcggaaggt ctgaacctgc agacggcgga tttcctggtg aacttcgatc tcccatggaa
     7741 ccccatgaag gtggagcaga ggatagggcg catcgaccgc attgggcaaa ggcacaagac
     7801 catctatgtc tccaacctgt gctatgtggg ttccgcagag gaaatcgtct acggccgcct
     7861 ctggaagcgc ctcaccgatg ccggagcggt ggttgggaca cagcagatct ccctccttcc
     7921 ggtcacaacg gaagagtttc atgaactggc cgagaaccct gcctttgcca agaagctgga
     7981 gaaagttgcc aaagagcggg ccatcctttt ccagaagcat accgcatcac gggagattcc
     8041 gcctgatgag cttttcgaga tctatgaaag gatcgagcag gacgaatcgg cgaggaaagt
     8101 gccggtaggt ctggacgaca tatgggaagc tctgtcgaac tcgacctatc tgcgtgaatt
     8161 gggatgtgcc cctgtcttcc acgcagagca aagagtcctc aaagtttcca acatccccgg
     8221 ggtgtcccag gattcagccg tcacaacctc caggacgacc tatgaggttg gcgttaccga
     8281 ccttgaggaa cgcctgcatt tcgcaaccta tggggatgcc gtcttcgatg cgatcctggg
     8341 tcacgtgggt gaattcgagc tgccgggttg cctatgcagg ctggaaacca gagttcccgg
     8401 cctcgagacc gagatgatcg cttacgctgc ggcatccatc gacgagcacg gcaatcctgc
     8461 atgcaggctt ctcacgtcgt atcaggacct gaacggcctt tatctcgatg aaacccgaac
     8521 actcacggac gacgacgtga agtcggctct ggaagccctg cagcggcagg cggttgagga
     8581 ttttcgaacg catctggcgg ttccaaggct caaagcggaa aacgaggtgt gtgccaactc
     8641 gcagttgatc cttgactatc tcaccatttc cgggctcatc cgagagcgcc agttcctcgg
     8701 aaagagtgaa acgcttttct ggcgtgaaat cgacgccatc catgaggagt acaaagaccg
     8761 ggagaacctc cgcatccgca aaatcccagc gaagatcgca tcccgcctct ccgggctcct
     8821 cttcgacgtc atcatccccc aaatgggaga agaggccttc atcgacgctt cccaaccact
     8881 cctaaaagct gccatcgatg ccatctgccg tgtcgcagac gccatgaagg tcaagaaatc
     8941 cgagctcacc accgacgacg tcctggctag agtcgacaga ctggttcagc agggttttgt
     9001 gtgaagagac tctttgtcgg acgtggacag ggttggaact ggggtgttaa gcttcagacg
     9061 gaattggagt tttccttcgt atgcgttaac caccggcgac ggaggaaaat ggaatgagca
     9121 ggaaacgcac gaattacagt cccgaggaaa aggccctctg tgccgatgtg acggagattc
     9181 cttccctgtg ataggtgttg agggtgggga aggaggcgga catgagaaac agccggctcg
     9241 acaacgggaa cctcgacaag gcgcgccgct tgctttggcc gatcaaacgg aaatgcggtg
     9301 ggaagatttc atgggccgac cccgtgatcc ttgcgggcaa ggtcgctccg gagtcgatgg
     9361 gattcaagac cttcggcttc ggcggtggcc gcgaggatgt ccggaagcag ccccgaatag
     9421 ggtctcggct ggaagagcag cttcggcacc ggcaaaggcg ccgataccat taccagcggc
     9481 ctcgaggtca catggaccgc cacgccgacg aagcgggccg catcgaacct tgagggggat
     9541 tgcccgcgct aaacggccgg ctttctcaac catcatccac aaaggaaagg accttatgac
     9601 cagtctcaga ggaaccgcaa cggaaccaaa cctgttaaag tccatttgcc ggtgagagcc
     9661 aggcgcgaaa tcggtacacc taatcctgct tgccttggag gggtccgatc ggggaaggac
     9721 ggcgagaagg cgggtgcttc attccccttg cccgtcaccc cgaaccttcc cacaagaaag
     9781 gagcgatcat gcgaaggaga gtgatcccga gtttgaccgc cgccgtccta ctgctgcttc
     9841 cgtgtgcggg cctggcggac cgctaccaaa tcgatccggc ccacaccagt ctcaccttct
     9901 ccgtccgaca ttttgggatc agcaacgtga aggggcattt tgacgagttt tcgggctcga
     9961 tcgtgttgga aaacggggcg atccgggagg cctccggcac gatccaggtc aacagcatca
    10021 acacgggcgt tcggcagcgg gacgatcatc tgcggacggc ggattttttc gacgccccca
    10081 actacccgca gatccttttc aagaccggga gggttgaaaa gagcggtgaa gaaaccgtcc
    10141 tggtcgggga tttcaccatg cggggggtta cccgggagct gcgattgccg gtgcggttga
    10201 acggccccat caaggacccc aagggaaacc tccgcattgg gctggaagcc aaaaccggcc
    10261 tgaatcgaaa agactacgga atccgctatc atgccgccct ggagaccgga gtgccgcttg
    10321 tcggggacga ggtcgccatc gaaatcaacg ccgaagcgat caaggaagtc cccggcgaaa
    10381 cggggaccca gtgagagacg gatacccggc gctttcccgg atttccgccc gcaccccgta
    10441 ccccgccccg gcggctagtc ggccgaaaac acggcggtca ccgacccgtt tccgtgaagc
    10501 tggtattggc gaggtgaaag ccctgcagct ccacgtcgct tttctcacat cccagggcat
    10561 gagtcgggtg ttctttgacc ggcggatggg agagaagtcc gcttccttta aggggtgtgg
    10621 tccgggcgaa gacggtgttc cttttgggag cttcaaaggg acctttgtgc cgcgtccacg
    10681 aaccgggcga tttccttttc aagacgggca atgatcgacg aggtgtcccg ggtgctgcct
    10741 gcctggccca gggcttccag ctgccgacac agggctgcgg gtatttttgc ccccaggttg
    10801 gcgctgctgg atttcaggct gtgggcggcc cgcctcagcc cgttccagtc gtcggacgcc
    10861 gcagcctccc gcaagtctcg aacaaggctc ggggtatttt ccacatagag gccgatcaac
    10921 atggtcagaa agtcggggtc atctttctgt tcgagttccc tgagagcgtt ccacacttcc
    10981 cagtcgatga aatccccatc gggagcgtct ccttcccggt cttggctcgt tgtggaggtg
    11041 ggcggctccg ggcgtgagtc catatcctct cccgccggag aagacccgga cgaaggtccc
    11101 gaagcagggg accgcccggg aaagcgtttg gggagccact gctccagaat gcgacccaac
    11161 tcctcccggg tgaagggctt gctgagataa tcgttcatgc ctgcggcaag gcatttttca
    11221 cggtcccctt ccatggcgtg tgccgtcagg gcaatgacgg gaatctcggg ccttccgctt
    11281 tgccggagga gccgggtagc ctcgtagccg tccatttccg gcatttggca gtccatgagg
    11341 actgcgtcgt aggaacctcg ggaaacggcc tccagggcct ctctcccatc gtttaccacc
    11401 accacggtgc atcccaggct ttccaacatg ccccgggcca cctcctggtt gacgggatta
    11461 tcctctgcca ggagaacatg accgctcagc ccggccgggc cgggagtcga cgggatttcg
    11521 acctcgcggg gctccgttgc ccgggccgac gggacgctca ggacggacac aatggcgtcg
    11581 agaagctgcg actgccgaac gggtttggtc acgcaggcga ggacgcccgc ctcccgggtt
    11641 ctttccatct cttcgatctc cccgacggac gtcatcagga tgatacgagc gccggatagg
    11701 tcgggatcgg accggatggc tcgggccagt tcgaggccgc tcatccccgg catcgtcctg
    11761 tccaggatga ccacgtcgta ggcttcgccc cgctcatagg cccgccgcat cttttcgagg
    11821 gcctgggccc cgtcttcggc gcacccgttt gccatgcccc aggacaccac ctgctggtgg
    11881 aggattttcc gactgacggc attgtcgtcc acaatcagta cccgaatatt cttcaaatcc
    11941 cggacggctt cctcctgggg gcgccgggcc gacggttgct tcttcagctt ggccgtaaag
    12001 gagaaggtgc ttccccggcc cacctcgctc accaccgaaa tccggccccc catcatctcg
    12061 cagagctgct tggagatggc caggcccagt cccgtgccgc cgaacttgcg ggtcatggag
    12121 ccgtccgcct gggagaaggc gtcgaagatg taggcaagcc tttcgttggg aatcccgacg
    12181 cccgtatcgc ggacgttgaa ttcgagaaca acggagccct cgtcttgttc cgac
//
LOCUS       DAOOVC010000114        12129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3426_length_12129_cov_6.938794, whole genome shotgun sequence.
ACCESSION   DAOOVC010000114 DAOOVC010000000
VERSION     DAOOVC010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3426_length_12129_cov_6.938794"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLM79_13165"
     CDS             <1..365
                     /locus_tag="PLM79_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOV87307.1"
                     /translation="EDLCVAAADATMNVMVEVLDTIEPRVTKELCLEADTAEMLLFEL
                     LQELIFWKDAEQLLLRVIAARIRQEEGRFFLVARAAGERLDPEKHELVVDVKAVTFHR
                     YRVEPAARGWEATVILDI"
     gene            548..1567
                     /gene="tsaD"
                     /locus_tag="PLM79_13170"
     CDS             548..1567
                     /gene="tsaD"
                     /locus_tag="PLM79_13170"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOV87308.1"
                     /translation="MIILGIETSCDETAAAVVKDGTVVLSDVVASQVAVHTPYGGVVP
                     ELASRKHVEAILPVIGRALEQADLTREAPDAVAVTQGPGLVGALLVGLSAAKAMSYAL
                     DKPLIAVNHLEAHMHAAFLGKGPPEKPLVCLVVSGGHTALYQVDPGGGARLLGGTRDD
                     AAGEAFDKVAKLLGLGYPGGVVIDRIAPQGNPQAFPFPKAFMEKESLDFSFSGIKTAV
                     ANFVRRHGLPGSGPQGGPYRLEDLAASFQEAVVEVLVTKVVRAANGAAVRDVAVVGGV
                     AANERLRMRLREEAAYHGLTLHLPESRYCTDNAVMVAAAGYYAWKRAGFCPNPLDLDA
                     RSRWY"
     gene            1577..2437
                     /gene="rsmA"
                     /locus_tag="PLM79_13175"
     CDS             1577..2437
                     /gene="rsmA"
                     /locus_tag="PLM79_13175"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOV87309.1"
                     /translation="MLISPNQYYRMTGIKSQKPLGQHFLTQPCTALRIVESAQIRDSD
                     VVVEVGPGLGALTQFLLPKGCALHLVELDGNLASFLREAVSRRSPAVQIHEMNVLDFD
                     FRTLARSVGQSLVVVGNLPYNISSPLIFSLLEAHPAFSRAVFMVQKEVGLRLAADPGT
                     GEYGVLSVLLGMYAQTKILFGVGPGQFFPRPKVDSLVVRMDFSGEGLAGELPFSFLRI
                     LVSTAFQQRRKTLRNSLKDFSLRRGAKVEEVLSLAQIDPGRRPETLSPLDFVRLGAVL
                     KGSRNPVVAG"
     gene            complement(2506..3429)
                     /locus_tag="PLM79_13180"
     CDS             complement(2506..3429)
                     /locus_tag="PLM79_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HOV87310.1"
                     /translation="MDIHRLEVFCKVVDLQSFTKAAEAVYLTQPTVSEHIRALEEMVG
                     EKLVDRLGREVLPTPAGKILYQYARTMIQVRDEAIQALESYKGNLSGHLSVGASTIPG
                     TYILPEMLGSFKSLFPSIQTTLIISGSSQIISRLLDGSIELGLVGAKWSDRRILSEEI
                     FSDELVLTVYPEHPWAGLGEISLQQLAGEPFIQRERGSGTRIVMEQALSSRGFDPAQL
                     HVIAEMGSTEALRQGIKARIGISILSYQAVAEDADRGTLVTVGIEGLRLFRPFYLVRR
                     KKRHPSPLGTAFVEHLRTQAADRLNRRADPE"
     gene            complement(3472..5493)
                     /locus_tag="PLM79_13185"
     CDS             complement(3472..5493)
                     /locus_tag="PLM79_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOV87311.1"
                     /translation="MLSVQGISKSLGKKELFRDVSFHLHRGEKVGLIGPNGSGKTTLF
                     HIMLELAEPDSGTIVKSKCLRCGHLPQQWIPLQGKTVLAYAMDVDQQVRSVRSRLRDL
                     QARLEKETDRERIEELALEQTGLLERLEHLGGYDLEARAAKVLAGLGFDDRQMHQSVA
                     GLSGGWGMRLELARLLLEEPDLLLLDEPTNHLDLESLMWLESYLVGTASSVVIISHDR
                     TFLNRVVQRIFELDQGRFQEYRGTYDSYLEEKARRKEILTATYKNQQERIRQVERFIE
                     RNRVRKDRAKQVQSRLKLLEKMEQVGPVREEAHVRFSFPEPPRSGRNVFELSKVHKAY
                     GDHSVYAGIDLTIERGERVAFLGANGAGKSTLLKILAGVETITGGERLLGHNVVLGYY
                     AQYQWEQLHADRTVLEEASSVAGDLTQTALRSLLGAFLFRGDDVSKKIAVLSGGEKAR
                     LILCKLLLQRPNVLLLDEPTNHLDIPSRVVLETSLADFPGTLCFISHDRHFINAVATQ
                     TLLVEEGRVQLFPGNYDDFQDVWKSRVRPEAPHATEAAPREQTAPAELSPKKDPSRKR
                     AEAQWRNELYRLKKPLQERLDQTESRLEAAQTRWQELNKALALPDTYRDSAKAQELIR
                     EHRECQARIEQLTQVWEEAALAIEDLEAGFRQQKEDPDRERGGKSSSIS"
     gene            6043..7233
                     /locus_tag="PLM79_13190"
     CDS             6043..7233
                     /locus_tag="PLM79_13190"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOV87312.1"
                     /translation="MRVPAGEIARRLVAFQELLSRSGIDAAIIRQNTDLYYFTGTVQD
                     AHLIVPASGRPLFMVRRDPERALSQSSLRPIHPLRSIRALPAAVREACGTESPRVLGL
                     ELDVLPANTFFFYDEKLFPKQQIRDVSGLIRRVRMIKSPWELEMMQGAAGLSRTVSDA
                     VPRFLREGISELELSIELEAVARRAGHLGLIRLRGFNMDMYFGHVLSGPEAAVPAYSD
                     TATGGLGLSPAFGQGPSLRVIRAREVVSVDTMMNYHGYLNDQTRNFCIGEPPERLQKA
                     YTFVREVHRRFREIARPGSVCAELYHKVCEWAEAEGWTEYFMGYGEDRVSFVGHGLGL
                     EVDELPLIARGQETILEAGMTFAFEPKIILPGTGIAGLENTYVVDPDGLRSLNTAPED
                     LMLL"
     gene            complement(7798..10686)
                     /locus_tag="PLM79_13195"
     CDS             complement(7798..10686)
                     /locus_tag="PLM79_13195"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87313.1"
                     /translation="MKISSRLYLILGTVFLLCACSIVFLVSHQMRNQALHEAESKALI
                     ILDRDLATHDYFQHELKPKLFKWSDAFRSEDFFDPSWMSSTYVIRQIDRNFKLLNPGN
                     YYDKTCAVDARNPENEADDYERPFLEELNRNPSVVARSCVRFFDGQPYFVVMRRGLLM
                     EQHCLKCHGNPKDAPRDMIRHYGAERSFHRRVGETVSAISIRIPLTAAYRQTEAFSWK
                     LSGILLIALAFLFIVQHWVNNRLLLKPLGRMHEKALQIAASDEHLGEEIPLPQGQELR
                     ELTEAFNLMSERLRERNDELRRLNIQLQLDIARRERAEEALRESEACLCAAIESLPFD
                     FWIRNPRGQFVLQNSKSRENYGDMIGKQPEEVCVDEAVLAAWQRTFQRAMAGELTQEE
                     VEYRTPAGKKTYHNVVAPVLDGEQTLGILSANIDITERKNAEEELLRYREHLENLVEY
                     RTTELRMANEQLRQEVAERRKIEEVLRESEERYRSLIETAHEGIWVIDADGNTTFIND
                     KMTHLLGYSAEEIAGKSFFDFMNDEWKTVAESFLERRRGGIREQLDFKFLRKDGSELW
                     AIVSTNPLFAKDGRYAGALALITDITDRKAAEEALLKSEEKFRNIYQESPIGIELYNA
                     RGELIDINRSCLEMFGILHEEEVLGFQLHRDPNVPEEIMNRVRRGEMVRFETTFDFDK
                     IREMNLYATHKQGIAWLDVLITPLRTAGILGGYLVQIQDITERKRSEQTLRESEKQLR
                     FLSTQLLSAQEEERVRMAREVHDSIGSSLTGIKISLENLLCDFEKGVLKPESIQVLIT
                     TAQNSIRECRRIMTDLRPSILDDYGIVATTEWLCEQFQAIYRNILIHKTLDIQEKDVP
                     ENLKIILFRIMQEALNNTAKYSKAQSVHLLLRKRPECIELRIEDDGVGFDPASVLAKS
                     NCEKGVGLTSMKERAELFGGIFSIFTAPGKGTSIRVSWPCETLAAVAC"
     gene            complement(10816..>12129)
                     /locus_tag="PLM79_13200"
     CDS             complement(10816..>12129)
                     /locus_tag="PLM79_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOV87314.1"
                     /translation="PNYANVSSYVALTGRTVNIEDVYETEIFDFTGPKKYDASTGYRS
                     KSMLVIPMKNHEDDIIGVLQLLNALDPETGEVIGFSQELVSLIASLASQAAVALTNAQ
                     LIQDLRNLFYSFIRSIATAIDEKSRYTGGHIRRVVDLTMMIVEAVNETRQGPLAAVHF
                     SADEVEELRLAAWMHDVGKITTPEYVVDKSTKLQTIFDRIELLETRFDLVGKTIEEQY
                     LLEKLQGLSREKPPAPCLEDPDSKRENALQALEQDRAFVRGCNDPDRFLSDADLERLR
                     QIARKTYRLNGRVLPYLTEDELYNLSTRKGSLTEKERETIRQHVVMTRRILSEIPFPK
                     KLRHVPLYAAGHHEKLDGSGYPLGLKVHQLPIQARIIAIADVFEALTARDRPYKDPLT
                     LPQAMKVLQSMSDAGHIDPHLFQVFVDSGIHQHYADKELTSPETP"
BASE COUNT         2310 a         3469 c         3690 g         2660 t
ORIGIN      
        1 tcgaggattt gtgcgttgcg gcggcggacg ccaccatgaa cgtcatggtg gaggtgctgg
       61 ataccatcga accgcgggtg acgaaagagc tttgcctgga ggccgatacg gcggagatgc
      121 tcctcttcga actcctccag gaactcatct tctggaaaga cgccgagcag ctgctcctcc
      181 gggtgatcgc tgcccggatc cggcaggagg aaggccgatt ctttctggtt gcccgggcgg
      241 cgggagagcg ccttgacccc gagaagcatg aactcgtcgt ggatgtgaag gccgtaacct
      301 ttcatcgcta tcgggtggaa ccagctgccc gaggctggga agccacggtc atcctggaca
      361 tttgagaaga tgagccgtgc agtcgagggg gaacatcggt ctttgctgaa aaggacgcgg
      421 ccattgtttt caacgggcct tcaaacgtgt taccttcgga tggaccgaga cggctgccgc
      481 cgcacggcgt actgaaagta gggcctcatc gaggcaaagg cagggagcgg gagggcagga
      541 acagggcatg atcattctgg gcatcgagac ttcgtgtgac gagacggcgg cggctgtggt
      601 caaggatggg accgtggtgc tgtccgatgt ggtggccagc caggtggcgg tgcatactcc
      661 gtacggaggg gtggtcccgg aactggcttc ccgaaagcac gtggaagcga tccttcccgt
      721 catcggccgg gccttggaac aggcagacct cacccgggag gccccggatg ccgtggccgt
      781 gacccagggc cctgggctgg taggtgccct cctggtcgga ctcagcgccg ccaaggccat
      841 gtcctatgcc ctggacaagc cgctgatcgc cgtcaatcat ctcgaggcgc acatgcacgc
      901 agcctttctg gggaaaggcc ccccggagaa gcccctggtt tgcctggtgg tttccggagg
      961 gcacacggcc ctgtaccaag tggaccccgg ggggggggcc cggcttctgg gcggaacccg
     1021 ggacgacgcc gccggagagg ccttcgacaa agtggcgaaa ctcctgggcc ttgggtatcc
     1081 cggcggagtc gttatcgaca ggatcgcccc ccaaggcaac ccccaggctt ttccttttcc
     1141 caaggccttc atggaaaagg aatccctgga tttcagcttc agcgggatca agaccgcggt
     1201 ggccaatttc gtccgtcggc acgggctccc cggttccgga ccccaagggg ggccttatcg
     1261 cttggaagac ctggcggcga gttttcagga ggccgtagtg gaagtcctgg taaccaaggt
     1321 cgtccgggcc gccaacgggg cggcggtccg ggatgtggcc gtggtggggg gagttgccgc
     1381 caacgagcgc cttcgaatgc gcctgcggga ggaagccgcc taccacggat tgacccttca
     1441 tcttcccgaa tcgcgctatt gcaccgacaa tgcggtcatg gtggccgccg ccgggtatta
     1501 tgcctggaaa cgtgccggat tttgcccgaa tcccctggac ctggatgccc ggtcccggtg
     1561 gtattgaccc gaccccatgt tgatcagtcc caaccaatac taccggatga ccgggatcaa
     1621 gtcccaaaaa cccctggggc agcatttcct gactcagccg tgcacggccc tgcgcattgt
     1681 ggagagcgct caaatccggg actccgacgt ggtggtggaa gtgggccccg ggttgggggc
     1741 tctgacgcag ttcttgcttc ccaagggatg cgccctccac ctggtggaac tggacggaaa
     1801 cctggcgtcc ttcttgaggg aggccgtgtc ccggcgttcc ccggcagtcc aaatccatga
     1861 aatgaatgtg ctggattttg acttccgcac attggcccga tcggtcggtc aatccctggt
     1921 ggtggtgggc aacctgccct acaacatctc ctcccccctg atcttttccc tgcttgaggc
     1981 ccatcctgcc tttagccggg ccgtcttcat ggtgcagaag gaagtggggc tgcgcctggc
     2041 tgcggatccc ggcacggggg aatacggggt cctttcggtt ctactgggaa tgtacgcaca
     2101 aacgaagatt ctcttcggtg ttgggccggg ccagttcttt ccccggccca aggtggactc
     2161 gttggtggtg cgtatggact tctcggggga aggcctcgcc ggggagcttc ccttttcatt
     2221 ccttcgaatc ctggtgagca ccgcctttca gcaaagacgc aagactctcc ggaacagtct
     2281 gaaggatttt tcgttgcggc ggggggctaa ggtcgaggaa gtgctctccc ttgcccaaat
     2341 cgatccgggt aggcgccccg agactcttag ccccttggat ttcgtccggc tgggggccgt
     2401 cctgaaaggg tcccgaaatc ccgtggtcgc gggataagcc tgcatcgccc ggaccaaacg
     2461 ccgtacgggg gaatcccgga ccttttccga cccgagggga ttcccttatt cgggatccgc
     2521 cctgcggttc agccggtccg cggcctgggt gcgcagatgc tcgacaaagg ccgtgcccag
     2581 gggggaggga tgtcgcttct tccttctcac caggtaaaac gggcggaaga gccgcagtcc
     2641 ctcgataccc accgtcacca gggtcccccg atccgcgtct tcggctacgg cctggtagga
     2701 gaggatggaa atgccgatcc gggccttgat gccctggcgc agcgcttccg tacttcccat
     2761 ttccgcgatg acgtgaagtt gagccggatc gaaccctcgg gaggagaggg cctgttccat
     2821 gacgatccga gtgcccgaac ctctttcccg ctggatgaag ggttctcccg cgagctgctg
     2881 cagggagatt tcccccagac ctgcccacgg gtgctcggga taaaccgtca agaccaactc
     2941 gtccgaaaag atttcctccg aaaggattcg ccggtcgctc cacttggctc ccacaagtcc
     3001 cagctcgatg cttccatcca aaagtctgct gatgatctgg gagctgcccg agatgatcag
     3061 ggtggtctgg atggagggaa agagggattt gaaggacccg agcatttccg gaagaatgta
     3121 ggtcccggga atggtgctgg cgccaacgga caggtgcccg gacaggttgc ctttatagct
     3181 ttccagggct tgtatcgctt cgtctcttac ctggatcatg gttcgggcgt actggtacag
     3241 aatcttgccc gcaggggttg gaaggacctc gcgtcccaac cggtccacca gcttctcgcc
     3301 caccatttct tccagtgccc ggatatgttc gctcaccgtg ggctgcgtaa gatacaccgc
     3361 ctcggccgcc ttggtgaagc tctgaagatc gactactttg cagaacactt ccaaacgatg
     3421 aatgtccatc aattccgctc ccgatgctgc gagaggacgt gcgctgccgt ttcaagaaat
     3481 gctcgaactc tttccccccc gttcccggtc cgggtcctcc ttctgctgcc ggaagccggc
     3541 ttccaggtcc tcgatggcca gtgccgcctc ttcccatacc tgggtgagct gctcgatgcg
     3601 ggcctgacac tcccggtgtt cccggatgag ttcctgggct ttggcggagt ctcgataggt
     3661 gtcggggagg gccaaagcct tgttgagctc ctgccaccgg gtttgagccg cttccagccg
     3721 ggactccgtc tgatcgagcc gttcctgcag cggcttcttc aagcggtaga gctcatttcg
     3781 ccattgggct tccgctcgtt ttcgggatgg gtccttcttc ggcgacaatt cggcgggagc
     3841 cgtctgttcc cggggggcgg cctccgtggc atgtggagcc tcgggacgaa ccctgctttt
     3901 ccacacgtcc tggaaatcgt cgtagttgcc gggaaaaagc tgtacccgac cctcttccac
     3961 cagcagggtt tgagtggcaa cggcattgat gaaatgccga tcgtgactga tgaagcagag
     4021 ggtgccggga aaatccgcca gggaggtctc caggaccacc cgggacggaa tgtccaggtg
     4081 gttggtgggc tcatccagga ggagcacgtt gggacgctgc agaagcagtt tgcagaggat
     4141 gagtcgggcc ttttcacctc cgctcagaac tgcaatcttc ttggaaacat cgtctccccg
     4201 aaacagaaag gctcccagca agctgcgaag ggccgtctgg gtcaagtctc cggcaacgga
     4261 ggaagcctcc tcgaggaccg tccggtcggc gtgcagctgt tcccattgat attgggcata
     4321 gtaccccagg acgacgttgt gtcccaggag tctctctccc ccggtgattg tttccactcc
     4381 ggccagaatc ttcaacaggg tgctcttccc ggcgccgttg gcgcccagga acgccacccg
     4441 ctccccccgc tcgatggtca gatcgatccc ggcatagacg ctgtggtccc cgtaggcctt
     4501 gtggaccttg ctaagctcga agacattcct cccggacctg ggaggttccg gaaaagaaaa
     4561 ccggacgtgg gcctcctcac gaactgggcc gacctgctcc atcttctcca ggagcttcaa
     4621 acgactttga acctgtttgg ctcggtcttt ccgcacccgg tttcgctcga tgaagcgttc
     4681 cacctggcgg attctttcct gctggttctt gtaggtggcg gtgaggattt ctttcctccg
     4741 ggccttctct tccaggtagg agtcgtaggt tcctcggtat tcctgaaacc ggccttgatc
     4801 gagctcaaaa atcctctgga cgacccggtt gagaaaggtg cggtcgtggg aaatgatcac
     4861 cacggaagaa gcggtcccca ccagatagct ttcgagccac atcaaggact cgaggtccag
     4921 gtggttcgtg ggttcgtcca agagaagaag gtccggttct tcgagcagga gacgggccaa
     4981 ttccagccgc atgccccagc cgccgctaag acccgctacg gactggtgca tttgccggtc
     5041 gtcgaacccc aggccggcaa gaaccttggc tgcccgggct tccaggtcat atccccccag
     5101 gtgctcgagt cgttccagca gtcccgtctg ttcgagggcc agttcctcga ttcgctcgcg
     5161 atccgtctcc ttttcgagac gcgcctggag gtcccggagc cggctgcgaa cggaccggac
     5221 ctgctgatcg acatccatgg cgtacgccag gaccgtcttc ccttgcagag gaatccattg
     5281 ctggggaaga tgaccgcaac gcaagcactt ggacttgacg atcgtgcccg aatcgggttc
     5341 cgccagttcg agcatgatgt ggaacagggt agtcttcccc gatccgttgg ggccgatgag
     5401 cccgaccttt tccccccggt gcaggtgaaa ggaaacatcg cgaaacagtt cctttttccc
     5461 gagggatttg ctgattcctt gaaccgacaa cacgctgtat gccgctccta cgattggatg
     5521 tgccgcaaag cttttcaaga aaacgatatg acataaccgg tttttaggaa acagaaaacc
     5581 gcgggaggcg gaatgccgga gccgggaatc ccagccaaag cagccaaagg gtttacggag
     5641 cgtgctgaaa cgtgctaaac aggaggcagg tggtcggtgc cgagtggggg gggcattctc
     5701 acgggaaggc ggctctttag acgggcgtgc accctctgga ttcgcacgct tccatcgccg
     5761 gtgcgctttc ggacgcggcg actcatcggt tttcggcgac gcacagggcg ggtccccttt
     5821 tccggcatct tgtgggcgag ggaatccact gcggctttca gccatggttt cagagtgccg
     5881 gattcccctg gggggccgcg atttcttgcc tcttggagga caaggtgtga aggggtctcg
     5941 gtcatcgagc acagggacgc ccgagagcgt gtgttggaaa cagaggctct cgattgagcc
     6001 tgaccgcact ttcggccttc ttcgggagta ggatggacca ggatgagagt ccccgccggt
     6061 gaaattgccc gaaggctggt ggccttccag gagcttcttt ccaggagcgg gatcgacgcg
     6121 gcaatcattc ggcagaacac cgacctgtat tattttaccg ggacggttca agacgctcat
     6181 ctcattgtgc ccgcctcggg acggcccctt ttcatggtga gaagggaccc ggaacgcgcc
     6241 ttgtcccagt cttccctgcg tcccattcat cctcttcgaa gcatccgggc acttccggcg
     6301 gccgtccggg aggcatgcgg caccgagtct ccccgcgtgt tgggactcga gctggacgtc
     6361 ttgcctgcca acacgttctt cttctacgat gagaagctct ttcccaaaca gcagatccgg
     6421 gacgtaagcg ggctcatccg ccgggtcagg atgatcaagt cgccgtggga actggagatg
     6481 atgcaggggg cggccggttt gtcccggacc gtctccgatg ctgtgcccag gttcttgcgg
     6541 gagggaatct cggagctcga gttgagcatc gagctcgagg ctgtggccag gagggccggt
     6601 catctggggc tgattcgtct tcggggattc aacatggaca tgtatttcgg gcatgttttg
     6661 tccggacccg aagcggccgt tcccgcctac tcggacaccg ccacgggcgg cctggggctg
     6721 agccccgcct ttggccaggg gccgagcctg cgggtgattc gagcccgaga ggtggtgagt
     6781 gtggacacta tgatgaatta tcacgggtac ctgaacgatc agacccgcaa tttctgcatt
     6841 ggtgagccgc cggagaggct tcaaaaggcc tacacgttcg tccgggaggt tcatcgaagg
     6901 tttcgggaaa tcgctcgacc gggatccgtc tgcgcagaac tctaccacaa ggtctgtgag
     6961 tgggccgaag ccgaaggctg gacggaatac ttcatgggat acggggagga cagggtctcc
     7021 ttcgtggggc atggattggg actcgaagtg gacgagcttc ccctcattgc cagggggcag
     7081 gaaacgatcc tggaggccgg gatgacgttt gccttcgagc ctaagattat cctgccggga
     7141 acggggatcg cgggtctcga gaacacgtac gtggtggacc cggacggcct tcgttccttg
     7201 aacacggcac cggaagacct gatgttgctg tgaagccgag ggtcctgccc cattgccccc
     7261 cgaaaccggg gggaacctcc tgttcatcgt acgagacgac atgcacctct acgacctgta
     7321 tgcggttggg agagcagaat atggtgtttg tatcccggtt taccgggtcc caagatgagc
     7381 aatatttccc gctgtatatc aagttgcgct taaggtcgcc ctaacatgct gagtgcagat
     7441 tacgagcaaa gggctgaatc ctgcggatgg gcagtttcgg cttgagtgca acttcgtata
     7501 aggggataca tacagcccgc cgcctacagg cgacccaatt ttccgcctgc acagaccatg
     7561 aatcatgcat aaagcccgtg ggctttacaa gcgaagcgtt ctgtagggac ggggccctcc
     7621 ctgcccgccg ggatcgccgt tggatgggga atgatcctca agaatcgctc ggttaccgtc
     7681 ggtcgcttcc gaaacaagcg atcggcctct agggatgagc gttgaaattt cccgtggttg
     7741 agccgccttg gcttccaccc ggccggcgac tcttctcccg gcgattgtcc taatgcccta
     7801 gcaggcaacg gctgcaaggg tctcgcacgg ccaggacaca cgaatggacg ttcccttgcc
     7861 cggcgcggtg aagatcgaga aaatgccccc aaaaagctcg gcgcgttctt tcatgctggt
     7921 caggcccacc cccttttcgc agttgctttt ggccagaacc gaagccgggt cgaatcccac
     7981 tccgtcgtct tcaatcctca attcaatgca ttccggtctc ttgcgcagga gcaagtgaac
     8041 ggattgcgcc ttgctgtact tggcggtgtt gttgagcgcc tcctgcatga tccggaaaag
     8101 aatgatcttc aggttctcgg gaacgtcctt ctcctggatg tccagggtct tgtggatgag
     8161 gatgtttcgg taaatggcct ggaactgttc gcaaagccat tcggtggtgg ccacgatgcc
     8221 gtagtcgtcc aggatggaag gccgcagatc ggtcatgatt cttcggcatt cgcggatgga
     8281 attctgggcg gtggttatga gaacctggat ggattcgggt tttagaacgc ctttttcaaa
     8341 gtcgcagagg agattctcga gactgatctt aatgcccgtc aaggaggatc cgatgctgtc
     8401 atgaacctcc cgagccattc ggaccctttc ttcttcctgg gcggacagca gttgggtgga
     8461 aaggaaacga agctgttttt ccgattccct caaggtttgc tcggaacgct ttctctcagt
     8521 aatatcctga atttggacca gataccctcc cagaatcccg gcggttctaa ggggcgtgat
     8581 caagacgtcc aaccaggcaa tcccctgctt gtgggtggcg tagaggttca tttcccggat
     8641 cttgtcgaag tcaaaggtcg tttcgaaccg aaccatttcc ccccggcgaa cgcggttcat
     8701 gatctcttcc ggaacattgg ggtcccgatg gagttgaaag cccaggactt cctcctcgtg
     8761 aaggattccg aacatctcca ggcaggaccg gttgatgtcg atgagctctc cccgggcatt
     8821 gtagagctcg attccaatgg gggattcctg gtagatgttc cggaatttct cttcgctctt
     8881 gagaagagct tcctctgcgg ctttacggtc ggtgatgtcg gtaatcaggg ccaaagcccc
     8941 cgcataccgc ccatccttag cgaacagcgg gttggtggag acaatggccc aaagctcgga
     9001 cccatccttt ctcaggaact tgaaatccag ttgctcccgg attcctccgc gccgccgctc
     9061 gaggaaactc tcggccaccg ttttccattc atcgttcatg aaatcaaaga aggatttccc
     9121 ggcaatctct tctgcagaat accccagcag gtgggtcatc ttgtcgttga tgaaggtggt
     9181 gttgccgtcg gcatcgatga cccaaatccc ctcgtgggcg gtttcgatga ggcttcgata
     9241 gcgttcttcg ctttccctga ggacttcttc gatcttcctt cgttccgcaa cctcctgcct
     9301 gagttgttcg ttggccatcc gaagctctgt ggttcggtat tccacgaggt tttccaggtg
     9361 ctcccggtag cgcagcagct cttcttccgc gtttttcctc tcggtaatgt caatgttggc
     9421 cgaaaggatc cccagggtct gttccccatc cagaaccggg gccaccacgt tgtgataggt
     9481 tttcttcccc gcgggggtcc tgtattccac ctcttcctgg gtcagctccc ccgccatcgc
     9541 ccgctggaag gttcgctgcc atgcggccag gacggcctca tcgacgcaca cctcttcggg
     9601 ctgttttccg atcatgtctc cgtagttctc tctgcttttg gagttctgaa gcacaaactg
     9661 tccccgaggg ttccggatcc agaagtcgaa gggcaagctc tcgatggccg cacacaaaca
     9721 tgcttcactc tcgcgcagag cctcctccgc ccgttcgcgt cgggcgatgt ccagctgcaa
     9781 ctggatgttc aggcgccgaa gctcgtcgtt acgctctctc aggcgctcgg acatcagatt
     9841 gaaggcttcg gtaagctcgc gcagctcctg cccttggggc aggggaattt cttcacccag
     9901 atgttcgtcg ctggccgcaa tctggagtgc cttctcgtgc atccgtccca agggcttcag
     9961 aaggagtcga ttgttcaccc aatgctgaac aatgaagaga aaggcgaggg cgatcaggag
    10021 tataccggag agcttccagg agaaagcctc cgtctgtcga taggccgccg tgagaggaat
    10081 acgaatggaa atggcggaaa cggtttctcc caccctgcga tgaaagctcc tctcggcccc
    10141 atagtggcga atcatgtccc ggggtgcgtc cttggggttc ccgtggcact tgaggcagtg
    10201 ctgttccatg agcaatcccc ggcgcatcac gacaaaataa ggttgcccat cgaagaaacg
    10261 aacgcaggat cgagccacca cactggggtt tcggttgagt tcctcgagaa agggcctctc
    10321 gtaatcgtcc gcctcgtttt caggatttcg agcgtctacg gcacaggtct tgtcgtagta
    10381 atttcccggg ttcaacaact tgaaattgcg gtcgatctgt cggatgacgt aggtggagga
    10441 catccagctg ggatcaaaaa aatcctccga acggaaggcg tcactccact tgaagagctt
    10501 cggcttgagt tcgtgctgaa agtagtcatg agtggccaaa tcccggtcca ggataatcag
    10561 ggccttggat tccgcctcgt ggagggcttg attgcgcatc tgatggctca ccagaaagac
    10621 aatcgagcac gcgcagagga gaaacaccgt tcccagaata aggtacagac gagaggaaat
    10681 cttcatggtt ggccaacctc ttcgatgggc tcaatcggta tgattcccaa aaccttgcag
    10741 cgagtataac aaatcgggga agaaccctcc aggggacgga atcgtcgggg cttgagggtt
    10801 ctcgacgctg gaatatcaag gggtttcggg ggatgtgagc tctttatccg catagtgctg
    10861 gtgaattccg ctgtccacaa aaacctggaa gaggtggggg tcaatgtggc ccgcgtcact
    10921 catggattgg aggaccttca tggcttgggg gagggtcaaa gggtctttgt aggggcgatc
    10981 cctggccgtc aaggcctcga agacgtccgc aatggcgatg atgcgcgcct ggatgggcag
    11041 ctggtggacc ttgaggccca ggggatatcc ggacccgtcc agcttttcat gatgaccggc
    11101 ggcatacaga ggaacgtgac ggagtttctt gggaaacggg atttccgaaa ggatcctgcg
    11161 tgtcatgacg acgtgctgcc gaatggtttc ccgctctttt tccgtcagac tgcccttgcg
    11221 cgtggacaga ttgtacagct cgtcctcggt caagtaaggc agcaccctcc cgttcaatcg
    11281 ataggttttg cgggcgattt gccgaagcct ctcgaggtcc gcgtccgaaa gaaaccggtc
    11341 ggggtcgttg cagcccctca caaaggcccg gtcctgctcc agggcttgca gggcgttctc
    11401 cctcttggaa tccgggtctt cgaggcaggg ggcaggtggt ttttcacggg agagtccctg
    11461 gagcttttcc aacaaatatt gttcttcgat ggtctttcca acgagatcga accgggtttc
    11521 caacagctcg atgcgatcaa agatggtctg gagcttggta gacttgtcca ccacgtactc
    11581 gggggtcgtg atctttccca catcgtgcat ccatgccgca agtctcaact cttccacctc
    11641 gtcggcggaa aagtgtacgg cggccaaagg gccttgccgg gtttcattga cggcctcgac
    11701 gatcatcatg gtaaggtcca cgactcttcg gatgtgtccc ccggtatacc gcgacttttc
    11761 gtcgatggcg gtggcgatgc ttcggataaa ggagtagaaa aggtttcgca gatcttggat
    11821 gagctgagca ttggtgaggg ccaccgcggc ttgggaagcc agggaagcga taagggagac
    11881 cagctcctgg gaaaagccga tgacttcccc ggtttccggg tccagggcat tgagaagctg
    11941 aagcaccccg atgatatcgt cttcgtgatt cttcatggga atgacgagca tggacttgga
    12001 gcggtacccc gtggatgcgt cgtacttctt ggggcccgta aaatcgaaga tctcggtctc
    12061 ataaacgtct tcgatgttca cggtccggcc cgtcaatgcc acgtaggagg aaacattggc
    12121 gtaattcgg
//
LOCUS       DAOOVC010000115        12114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3430_length_12114_cov_23.242060, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000115 DAOOVC010000000
VERSION     DAOOVC010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3430_length_12114_cov_23.242060"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..582)
                     /locus_tag="PLM79_13205"
     CDS             complement(10..582)
                     /locus_tag="PLM79_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87315.1"
                     /translation="MEIRLKEVEEALLATKGILDVRWIEEKDRTVIRGMEEQLCKQGL
                     GGLGKACNEGVFSVLSREFVCAVLNNNDFRHADVPCLSWVVDDLVIGEEITDLGKLES
                     LKAQKDIKIIGRNFVVYFDRVRQVRGREPSFVFRGLHFPEIEGIAAVRDVVSASPMGA
                     ADSYLKTLYGWDTEDPELGTILIGFNLRES"
     gene            893..3100
                     /locus_tag="PLM79_13210"
     CDS             893..3100
                     /locus_tag="PLM79_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87316.1"
                     /translation="MTRHEDPSPEKRRTARAVLVPLLGLCLVLLVMLALRMEAGQPYK
                     TYQKAYLEARADRLRSSLIATHSEEEIRAQVEALRRTPLRIKEIRPALTGRVERCLTC
                     HDGIEEISPSHPVDAFGCVTCHGGDGRGVTVEIAHRGLIGGRNPSDLAVIEQTCAKPG
                     GQCHAGRTFSVQNSADRVRSGVMATMSGVIASLRYSWAAQPSSIARYASVGVKASKGG
                     TGNRIAGLLTIPLMSPIPGINPTDARGFPIQFSGEAADDQWRKFCARCHLWNKREEGP
                     SAHSSGCAACHALYNADATYEGMDPTLPKDKPGYARQHRLTTHVPVEQCLRCHNRSGR
                     IGLSFTGLQEADGYGTPYRQGRPDLEALSGGRDVRRLVPDVHFEKGLACVDCHTGNDV
                     MGDGRIYSHMRDQVEIRCTDCHGTPSDPPPTRLQGAEDEEALWRARTLKLPGIEGKEV
                     GLTQRGTPLLNLWKESDRMILQGKLDQKEHPCPVITGSITHRIPGHGPDRMECSACHT
                     RWAPQCYGCHDYRRQGEPMLDSMLGIKTAGMWQETRDYYRFEKPVLGVNSRGRVSVFM
                     PGCQVVYTELGPDHQPVPGRDKMVFRGPGLEHGIVSTPISPHATRTEVRSCRECHADP
                     KTLGLGRGFFTAGRNWDENSFTSLVKPEINPMGFAWETLADPRGRPLAASTHPGARPF
                     NQEELKRILRVAPCLPCHGRYDDPIWENPREAFERARLPEHQEKVARVLEGAP"
     gene            3208..5049
                     /locus_tag="PLM79_13215"
     CDS             3208..5049
                     /locus_tag="PLM79_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87317.1"
                     /translation="MGGSHALSCSECHLGREDLPEKAGAHQGLVPNPSDFTRPHFPCG
                     RCHQDQIRWVNRTLMRTAAGIVNQTRFLWGAQPDPGARFGVVDVDHLRLLPEQEASGA
                     LVDDLLRRRCLRCHLGTSGSIASGSFPGEIPVEGAGCSACHVIRGNRGTAKEDPLPPP
                     GTKGGGKTHQITTAVPTSQCLQCHRGNYVGSDYVGLFERDHSAAYNFDAKDPDRPPQL
                     YTHAYHHLLPDVHHERGIHCIDCHPVEEIMGEGSVHSHAGEQVGVRCTDCHGRPGKPP
                     VPREVASSDQRALRAAKLNPSYDLFQGQKVLTTSKGHLLTSTFERDGEWFLLSKADGR
                     VHKIPLLGRSPPDKTPLDHQISEHMERMECSACHGAWTFRDLGFHLVRLDRADYNPWA
                     WLTRQGDPQAEDLLEKNLLKPEDRWDPPASRDWLTGASRPGIWLAGYSLRRWEGLVLG
                     VNERGMVSAMRPQYQYWVSWVDGQGKVLMDSVIPRAGDGRPATAWNSYTPHTIRRQTA
                     ACWDCHDNPGSLGLGQGLLREKDGKTVPLTRPWEDGLGIHFELDQVIDFLGRPLQRTV
                     RPGSRFLGEEAIPRMSAGNPRYMRDLLRYYEGKEAYGDPAGFTGPKR"
     gene            complement(5030..5416)
                     /locus_tag="PLM79_13220"
     CDS             complement(5030..5416)
                     /locus_tag="PLM79_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87318.1"
                     /translation="MSGLTVKQVARRELVAREFLCMLFLSVALMAVSLISPVGYAPLG
                     LEPQAGGEVSAPWLVVWLQVLLRYFPPLLAGIFFPLLILALITFLPWLPRAGEEIPRR
                     LYYWGIHQIAFWVLGGAFAFLTFWGL"
     gene            5971..6597
                     /locus_tag="PLM79_13225"
     CDS             5971..6597
                     /locus_tag="PLM79_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HOV87319.1"
                     /translation="MRLDSFLEHIHPARLSADRIRIRATFCLGGVAFFFFMALVLTGL
                     LLAVYYLPTPEGAREGLHDLNYVIPFGRLMRSVHFWAGQLMLLAVLFHMARVVAARAY
                     MPPRHLNWLVGVGLLVLVAALDFSGYVLRWDSQSFWAAQVMTQLLGQIPAVGDALQQL
                     LVGGPRMGEAGLLRFYVLHCLAGPALLLALIFYHFWRVRKDGRAKQAL"
     gene            7628..9259
                     /locus_tag="PLM79_13230"
     CDS             7628..9259
                     /locus_tag="PLM79_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOV87320.1"
                     /translation="MTKIVSHSFEEYLKLLESFHGNAAPGLIIGGFMVDLALKHLPEG
                     ILFDAVSETPACLPDAVQILTPCTVGNGWLKVMNLGRFALSLYDKYNGRGVRVFLDPK
                     KLEAWQEIKTWLFKLKAKSEQDKDRLLAEIKEAGTEILGIQPVQVQSSLLKKKSRGRI
                     ANCSLCGEPYPVSDGGICRACQGEGPYVIQESREQNIDGPMLKVVALEDAVGKTALHD
                     MTSILPGQSKGPAFLHGQRITIGDLCRLQQMGRQRIYVQEENPLQSEWVHENDAALAF
                     AEAMAGEGITFKTPPREGKINFFADRDGLFHVEEERLERFNTIPGVMCASQKGYNLVD
                     RGRGLAGTRAIPLFLSRTVFEKAMAVLDDGPLFRVLPVREAKVGILVTGSEVFQGLVE
                     DKFIPILTTKIEKFRCRVIRSLIAPDDRDAIRDGVKEILASGVDLLVTTAGLSVDPDD
                     VTRQGLIDAGATDLLYGAPILPGAMTLLARIGQVQVMGVPACALYFKITSFDLLFPRL
                     LAGLTVTRQDLAKMGHGAFCMECKTCTFPKCPFGR"
     gene            9686..11887
                     /locus_tag="PLM79_13235"
     CDS             9686..11887
                     /locus_tag="PLM79_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV87321.1"
                     /translation="MGVTRRDFLKIAGATAGGGIAGSQFVPSLKAMEKASRSKETRVP
                     VFCEMCHWTCGALATVRDGKVVKLEGNPLTPNSRGKLCGRGNGGIGLLYDPDRVKTPL
                     IRAGNRGDGKYKKASWDEALNYVAEKLEHIKKNYGPEAVSLFSVGSTGKHLLTLVAAY
                     GSLNFAQPSGNQCRGAREVGYILTYGDTPGSPERLDLANSKVIVLFGSHLGENMHNSQ
                     VQDFANGLGKGAKLIVVDPRFSTAAGKAHYWLPIKPGTDMALILAWTNILIREGLYDK
                     AYVDAYCIGFNELAEEVSRYTPEWAEKETEIPAGLIVETALEIGRYKPSVMIHPGRHS
                     SWYGDDVQRSRAMAILGAVLGTWGREGGIYLPTKASMKKVIKEDYPEPQKGPIVELCK
                     YPFAIKQGLILGSTTAIREATLTEQPYPVKGWIVLGANVVKAMPNEKETLEAINKLDL
                     LLTIEIMPLETAMVSDVILPSASYLEQAEELEIKKGRSLFVTMGRAAVPPMYESKDGY
                     TIAREIAKRIGVGDFFPWETLEEKNKFQCQALNLDYEELSEKGCIVIPNTDSPYITPE
                     NQPVFKTPSKKIELYSAQLAAKGFDPIPKYTPPQQPPEGHFRLVYGRSPVLSFTRTCN
                     NPILTELHNENSIWVNAKKARELGIKEGEYVVLENQDGVKSNKIRAKVTQRIREDCVY
                     MVHGFGSELKGLRNAYRKGADDQGLITRYTIDPISGSTGLRVNFVRILKEA"
     gene            11890..>12114
                     /locus_tag="PLM79_13240"
     CDS             11890..>12114
                     /locus_tag="PLM79_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV87322.1"
                     /translation="MPRYAMVIDQRRCVGCMACIVACKNENAVPAEHYRTRVVEVVEG
                     EFPNLRMELRSELCNHCDNPPCVYNCPTGAS"
BASE COUNT         2630 a         3381 c         3552 g         2551 t
ORIGIN      
        1 ggagaaaatc tagctttctc ggaggttaaa cccgatcaga atggttccca gttcagggtc
       61 ttcggtatcc catccgtaca gcgttttcag ataagagtcc gcggctccca tgggagatgc
      121 cgagacgaca tcccggactg cggcaattcc ttcgatttcc gggaaatgga gacctcgaaa
      181 aacaaaggaa ggttcccgcc cccgaacttg cctcactcga tcaaaataca cgacaaaatt
      241 cctgccgatg atctttatgt ccttttgggc cttgagggac tcgagcttcc cgaggtccgt
      301 aatttcctct ccaatcacga ggtcgtccac tacccacgac aggcagggca catccgcgtg
      361 ccgaaagtcg ttgttgttga gaaccgcgca cacgaattcc cgggagagga cggaaaagac
      421 gccctcattg caggcttttc cgagaccccc cagcccctgt ttgcaaagct gttcttccat
      481 gcccctgatc accgttcggt ccttttcttc aatccacctc acgtccaaaa tccctttggt
      541 ggccagcagg gcttcttcca cttcctttag acgaatctcc atggttcctt taccacccga
      601 acattgaaaa tcatggacgc acatcgaggg gcggccaaag cgcgtttcgc aggaagaaac
      661 caggcaaaaa ttttgcttgt ggatccctcc gtggttcgtt acttgtgggc cgtgagcctt
      721 cgcccatggg aaaaccgttg gttgcccggg gccgggttgg cagttaccta ttcccccgga
      781 tagaaaaggg gatggtcctc ttctgccgaa ccgataaggt cggcccgaat acgcccgtga
      841 acgaaggtca cgggcatcca ggctccccta cgccggactc aattttacta tcatgacacg
      901 acatgaagat cccagcccgg agaaacggag gactgcaaga gcggtcctgg ttcccctctt
      961 gggtttgtgt ctggttctcc tggtcatgct ggccctgagg atggaggccg ggcagcctta
     1021 caagacgtat caaaaagcat acctcgaagc ccgggcggac cgccttcgtt cttccttgat
     1081 cgcgacacat tcggaagaag aaatccgcgc ccaggtcgag gcccttcgac ggacccccct
     1141 acgaatcaag gaaattcgtc cggctcttac ggggcgggtg gagagatgcc ttacctgtca
     1201 tgacggaatc gaagagatca gcccgtctca tcccgtggat gcgttcggtt gtgtcacctg
     1261 ccatggaggg gacggtcgag gagtgacggt ggagattgct catcgagggc tcatcggggg
     1321 gcgtaacccg tcggacctgg cggtgatcga gcagacctgc gccaaacccg gggggcaatg
     1381 tcatgccggg agaacctttt ccgtccaaaa ttcggcagac cgggttcgat cgggtgtcat
     1441 ggccaccatg agcggagtca ttgccagcct ccgatactcc tgggccgcgc agccatcctc
     1501 catcgctcgc tatgcttccg taggggtcaa ggcctcgaag gggggaaccg gcaaccggat
     1561 cgccggcttg ctcaccattc ccctgatgag tccaattccc gggatcaacc cgacggatgc
     1621 ccgggggttc cccattcaat tttcgggaga agcagcggac gatcaatggc gcaagttctg
     1681 cgcccgatgt cacctttgga acaaacggga agaaggcccc tcggcccact cttcgggttg
     1741 tgcggcatgc cacgccctct acaacgccga tgccacctac gaggggatgg atccgacgtt
     1801 gccgaaggac aagccgggct atgcacgcca acatcggctc acgacccacg ttcccgtgga
     1861 acagtgtctg aggtgccata atcggtccgg acgcatcgga ttgtcgttta cgggcttgca
     1921 ggaagctgat gggtacggca cgccctatcg gcagggacgc cccgacctcg aggccttgtc
     1981 cgggggaaga gacgtccggc gccttgttcc ggacgtccat tttgaaaagg gactggcctg
     2041 cgtggattgc catacgggaa acgatgtcat gggggacggc cgaatctaca gtcacatgcg
     2101 agaccaggtc gagatccgct gcacggattg ccatggtacc cccagcgatc cgccgccgac
     2161 caggctccaa ggggcggagg acgaagaggc cctttggcgg gcaaggaccc tgaagcttcc
     2221 gggcatcgaa ggaaaagagg tcggtctgac ccagagggga acccccctgc tgaacctttg
     2281 gaaagaaagc gaccggatga tcctgcaggg aaagctggat caaaaggaac acccgtgtcc
     2341 cgtcattacg ggaagcatca ctcatcgcat tcccggccat ggaccggatc gaatggaatg
     2401 ctcggcgtgt catacccggt gggcccctca gtgttatgga tgccatgatt accgtcggca
     2461 aggggaaccc atgctcgatt cgatgttggg catcaaaacg gccgggatgt ggcaggagac
     2521 cagagactac taccgctttg aaaaacccgt gttgggggtc aactcccgcg gacgggtatc
     2581 cgtcttcatg cccggctgcc aggtggtata cacggagctc ggcccggatc atcaaccggt
     2641 gccgggccgg gacaaaatgg tttttcgagg gcccggcctt gaacatggga ttgtttccac
     2701 cccgatttct cctcatgcca cacgcacgga agttcgatcc tgccgggaat gtcatgccga
     2761 tcccaaaacc cttggactgg gccgggggtt tttcacagcc ggtcgaaact gggacgagaa
     2821 ttccttcact tcccttgtaa aaccggaaat caatcccatg ggatttgcct gggagaccct
     2881 cgcggaccct cgaggccgcc ccctggcggc gtcgacccat ccgggagcga ggcctttcaa
     2941 ccaggaagaa ttgaagagaa tccttcgggt tgctccctgt ctcccttgtc atggtcgata
     3001 tgacgacccc atatgggaaa accctcgaga ggcctttgaa cgtgctcgac tgccggagca
     3061 ccaggaaaag gttgcaaggg ttcttgaggg ggctccatga ggaccgcatc catcgcccgt
     3121 ttgattgtag ggttcggtat tctctgcctt agttccgcgg ccttcggaac ttcctcttgt
     3181 cttacctgcc acaaggagat ccggggaatg ggcggaagtc atgccctgag ttgctcggaa
     3241 tgccacctgg gcagagagga tctgccggaa aaggcgggtg cccaccaggg actggttccc
     3301 aatccttccg atttcacccg ccctcatttc ccctgcggcc gatgtcatca ggaccagatc
     3361 cgctgggtga atcgcaccct catgaggacc gccgcgggaa tcgtcaatca aacccgcttc
     3421 ctctggggag ctcaaccgga cccgggagcc cgttttgggg ttgtcgacgt ggatcacctc
     3481 aggctcttgc cggaacaaga agcctcgggg gctttggtgg acgatttgtt gcggcgtcga
     3541 tgcctgcgtt gccacctcgg gacatcgggg agtattgcct cggggagctt tcccggtgag
     3601 attcccgtgg aaggggcggg ctgttccgca tgtcacgtga tccgtggaaa ccgtgggacg
     3661 gcaaaggaag acccattgcc tccacccgga acaaagggag gaggcaagac ccaccagatc
     3721 accacggccg ttccgacatc ccaatgcctt cagtgtcatc gaggaaacta cgtgggatcg
     3781 gattacgtgg ggctttttga gcgggaccat tcggccgcgt ataattttga tgcgaaggac
     3841 cctgatcggc cgcctcagct ctacacccac gcctaccatc acctcctgcc ggatgtgcac
     3901 catgaacgag ggatccattg catcgactgt catccggtgg aggaaatcat gggggaaggg
     3961 agcgttcatt cccatgccgg ggaacaggtg ggagtccgtt gcacggattg ccacggtcgc
     4021 ccgggcaaac ctccggtgcc tcgagaggtg gcttcctcgg atcagcgagc gttaagagcg
     4081 gcgaagctga acccatccta cgatcttttc cagggtcaaa aggttctcac aacctcaaag
     4141 gggcatcttc taacgagtac gttcgagagg gatggagaat ggtttttgct ctccaaggcc
     4201 gacggaaggg ttcacaaaat tccgctcctg ggacggagtc ccccggataa aactcctttg
     4261 gatcatcaaa tctccgagca catggagcgc atggaatgtt cggcatgtca tggagcatgg
     4321 acttttcggg atctggggtt tcatttggtt cgcctggacc gggcggatta taatccctgg
     4381 gcctggttga cacggcaggg agacccccaa gcggaggatc tcctggagaa aaacctgctg
     4441 aaacccgagg accggtggga ccctcccgct tcccgggatt ggcttaccgg ggcgtcgagg
     4501 ccgggaatct ggcttgccgg ttattccttg cggcggtggg agggtctcgt cctgggagtg
     4561 aacgagcgag gaatggtgag cgccatgagg cctcagtacc agtattgggt cagctgggtg
     4621 gacgggcagg gaaaggttct catggattcc gtcattcccc gggccggtga cgggcgcccc
     4681 gcgacggcat ggaattccta caccccccat accataagaa ggcagacggc ggcctgctgg
     4741 gactgccacg acaatcccgg gagcttggga ctggggcagg gccttcttcg ggaaaaagac
     4801 gggaagaccg ttccgctcac caggccctgg gaggatggac tgggaatcca ttttgagctg
     4861 gatcaggtga tcgatttcct tgggaggccc ctacagcgca ccgttcgacc gggcagtcgc
     4921 tttttggggg aggaagcgat cccccggatg tctgccggga acccccggta tatgcgtgac
     4981 ctcttgagat attacgaagg caaagaagct tacggagacc cggccgggtt tacaggcccc
     5041 aaaaggtgag gaacgcgaaa gccccgccga gaacccagaa tgcgatctga tggattcccc
     5101 aataatacag ccggcgggga atttcctctc cggccctggg cagccagggc agaaaggtga
     5161 taagggcgag aatgagaagg gggaaaaaga tgcccgccaa caaggggggg aaataacgga
     5221 gaagcacctg gagccaaacg accaaccagg gcgcagaaac ttctccaccg gcctgaggct
     5281 ccagccccag gggagcatag ccgaccgggg agatcaacga caccgccatg agggctaccg
     5341 acaggaacaa catgcaaaga aattccctgg caacgagctc ccggcgagct acttgcttta
     5401 cggtgagtcc cgacatcccg cttcttctcc gattccgtca tcgtttgaaa gagacgactt
     5461 gctcaagaat tgaatctaac cgctttggga ttataggtca ttgaattccg aaataaaacc
     5521 tccggaggca gggaacagga gacgccgggt cttcctggcg ataggttccg ccttgatagg
     5581 gctggcctcg ggggggattc tgtggatgat cggttcgttt ctttccgggt ctcggaatcg
     5641 atcggagccg gaccctgttc attttgggaa cccggacacc tatcccgtag gcagtgtcgc
     5701 cgcccgcggg agggtggtcc ttctgaggga tgaagagggg ttctgggcgg ttaccgccat
     5761 ctgcacccat ctgggatgtc agcccacatt cgttccccaa gaacaccggt ttgtatgtcc
     5821 ttgccatggg agtcgtttcg atcccgaagg tcgggtattg tccggtccgg caaccaagaa
     5881 tctgtccctg gcaactatcc gcttggattc ccaggggttt cttgtagcgt accccgccga
     5941 aaaggcacaa cttggggata ggtttcgccc gtgagactcg attcttttct ggagcacatc
     6001 catcccgccc ggctttctgc agatcgaatt cgcatccgtg ccaccttctg cctgggagga
     6061 gtcgcttttt ttttctttat ggccttggtt ctcacggggt tgctgctggc cgtctactat
     6121 cttcccaccc ccgagggggc gagggaggga ctccatgatc tgaactacgt gattcctttt
     6181 gggcgactca tgcggtccgt ccatttctgg gccgggcaac tgatgttgct tgcggtgttg
     6241 tttcatatgg cgagggtcgt ggccgctcgg gcctacatgc ccccccggca tctcaattgg
     6301 ctggtgggtg tgggcctcct ggtcctggtg gcggccctgg acttttcggg atatgtattg
     6361 cgttgggatt ctcagagttt ttgggcggcc caagtcatga cccagttatt aggacagatt
     6421 cccgccgttg gagatgcgct gcaacaatta ttggtgggag gaccccggat gggagaagcg
     6481 ggtttgctgc gattctatgt cttgcactgc ctcgccggtc ccgccctctt gttggctttg
     6541 atcttttacc acttctggag agtccgaaaa gatgggcggg ccaagcaagc cctctgatgt
     6601 cttctcctgt ccgggttgtc tcgatacaga gatgtcacat gacaggatgt gtatcccgag
     6661 acaggggtgc aatggtttga agggacggca attggaagaa accggccaat tcttacattg
     6721 ccgttgtccc gtgatctggc accgaatttg ttaagaacta cggcgaggct gcgggcttcg
     6781 gtccatgctc tcggtgcgat cgggaagagt ttcggcttga cgggaaaggc tctgtcagag
     6841 aatcttaata agatttcaat aaggttgtgt ctaatgcagg aatttttcct ctagggaact
     6901 cctcggatgc gctggaaggc cgacggcaag gttccttcct tggcaggcgg atggattgcg
     6961 ggggggttcg tgtacccgga aaggagaaaa ggcgaatgaa gttgaaaaag gttcttttga
     7021 ttggtttgtt cgtatttctg gggataggag cggcctgggt gattacggga gtcagccaga
     7081 cgactcctcc cgcatcacaa accgccccgg tggaaacaaa agaaaaggcc aaaacacctc
     7141 ctcccgcgga tgcccaaaag gctactgctc ccacttcgga agcccccggt tccgttgcgg
     7201 ttccggcaac gggagaagcc ccgcctcccc gtaagtttag ccccagcgag ggatgttagt
     7261 tcttccggac gggaaattcc ttaccatgtg gggttgcaag aagtcttacg gttgggaaac
     7321 ctatcccgcc gattccatag aggggagctt ccgattcttt ggaggctcct ctttctcttc
     7381 tgatcccgac ccatgagatc cccccctcgt tcctcccgta tccgggtccc cgactccttt
     7441 ccgaagaagt cctagaaccc aagtcctccc ttgacctctt ggcggtcaat cagttatttg
     7501 cagcgggaat cggcccctta ggtcttgagg ggcaaggctc gagtcttttc cggtccggtc
     7561 cgggtgttcg cgattttatg ggtcctgctt ttccgatgct tcatcgggga aagggaaaac
     7621 tttcggaatg acaaaaatcg tttcccattc ctttgaggaa tatctcaagc tcttggaatc
     7681 cttccacggc aatgcggccc cggggcttat catcggcggt ttcatggtgg atctggcgtt
     7741 gaaacatctt ccggaaggaa tcctgtttga tgccgtctcg gaaaccccgg cctgtttgcc
     7801 cgatgccgtc cagattctga ctccttgtac ggtgggaaac ggatggctca aagtgatgaa
     7861 cctggggcgg tttgccttga gtctctacga caaatacaac ggtcggggag tgcgggtgtt
     7921 tctggatccg aagaaattgg aggcatggca ggaaatcaag acctggcttt tcaaactgaa
     7981 agcaaaatcg gaacaggata aggatcgtct gctggcggaa atcaaagagg ccggcacgga
     8041 aattctgggg atccagcccg ttcaggtgca atcctccctt ctgaagaaga agagccgggg
     8101 gcgaatcgcc aattgttccc tgtgcggcga accctatccc gtcagcgacg gcgggatatg
     8161 cagggcttgc cagggagaag gcccctacgt gatccaagaa tcccgggaac aaaacatcga
     8221 cggacccatg ctgaaggtgg tggccctgga agatgccgtc gggaagacgg ccctgcacga
     8281 catgacttcc atcctccccg gtcagagcaa agggccggcg ttccttcacg gtcaacgcat
     8341 caccataggg gatctttgtc gcctccagca gatgggccga cagcgaattt atgtgcagga
     8401 agagaatccg cttcaatcgg aatgggtcca tgagaacgac gccgccctcg ctttcgccga
     8461 ggcgatggcc ggagaaggga tcaccttcaa gacccccccc agagagggga agattaattt
     8521 ctttgccgat cgagacggcc tgtttcacgt ggaagaagaa cgcctggagc gattcaatac
     8581 gattcccgga gtcatgtgtg cctctcagaa gggatacaac ctggtggacc gggggagggg
     8641 tttggccggg acccgagcca tccccctgtt cctgtccagg accgttttcg aaaaggccat
     8701 ggccgtcctg gacgacggac ccctgtttcg ggtgcttccc gtgcgcgagg ccaaggtggg
     8761 catcctggtc acggggagcg aagtattcca ggggcttgtc gaagacaagt tcattcccat
     8821 cctcaccacc aagatcgaaa aattccgctg tcgcgtaatt cgatccctca ttgccccgga
     8881 cgatcgggat gccattcgcg acggagtcaa agaaatcctg gcttcggggg ttgatctgct
     8941 cgtgaccacc gcaggacttt ccgtggaccc cgacgacgtg acccgtcaag gattgatcga
     9001 cgccggggct acggaccttc tctatggagc tcccatcctg ccgggggcca tgactctgct
     9061 ggcccgcatc ggacaggtcc aggtgatggg agttcccgcc tgtgctcttt atttcaaaat
     9121 taccagcttc gatctcctgt ttccccggct tcttgccgga ctaaccgtta cacgtcagga
     9181 cctggccaag atggggcacg gagctttttg catggagtgt aagacctgca cctttcctaa
     9241 gtgccccttt ggccggtagc cgcactcttc aacccttccc ctcctcgtct cccccctccg
     9301 tctcacgact gcccggaaat ctttccgtgg tctaaacttt gctacgattt cctagttttg
     9361 aggtctgaaa ccggcctccg gtttttgcgc ttcaacggaa ggggcccgcc acccggtcgc
     9421 tccgttgaat ggttgaccgt agagcttctc ggttgattgc cttctgtgaa atagttatat
     9481 cctgctggaa tcggatgtta gaaagaattt agaagaactt ctcctgggaa cgggggaaaa
     9541 aggaagcgac cgaaatatcc atgatgccca tcccctcaaa agccgttccg gaaagtcgga
     9601 acctcgattt gcaaggcttc gttttgggac atgatggaat tccaggtgtg cgcctcgagg
     9661 tcaataggat acaggagaga aaattatggg agtaacccga agggatttcc tcaagattgc
     9721 cggtgcaacc gcaggtggag gtattgccgg ttctcagttc gttccatcct tgaaggcgat
     9781 ggaaaaagca tcccgttcaa aagaaaccag ggtccccgtg ttttgcgaga tgtgccactg
     9841 gacttgcggg gccttggcca cggtgaggga tgggaaggtg gtgaagctgg aaggaaatcc
     9901 gctcaccccc aacagcaggg gaaagctctg cggaagaggg aacggaggaa tcggcctcct
     9961 gtatgatccc gatcgggtca aaactccttt gattcgtgcc ggaaaccggg gggacggaaa
    10021 atacaagaag gcctcctggg acgaggccct caattacgtg gccgaaaagc tcgagcacat
    10081 aaaaaagaac tacggccctg aagccgtcag cctgttttcc gtcggatcta ccggcaaaca
    10141 cctcctgacc ttggtggcgg cctatggcag cctcaatttt gcccaacctt ccgggaacca
    10201 gtgtcgaggg gcccgggagg tcgggtacat cttgacctac ggagacaccc ccggttctcc
    10261 ggaacggttg gacctggcca acagcaaagt gatcgtgttg ttcggatctc acctcggcga
    10321 gaatatgcac aactcccagg ttcaagattt tgccaacgga ctgggcaaag gagccaagct
    10381 gatcgtggtg gatccccggt tctccacggc agcaggaaag gcccactact ggcttcccat
    10441 caagcccggg acggacatgg ccctcatcct tgcctggacc aacatcctca tccgtgaggg
    10501 gctttatgac aaggcgtatg tggatgccta ttgcatcggt ttcaatgagc tcgccgagga
    10561 ggtttcccga tacactccgg aatgggcgga gaaggaaacc gaaatccccg caggtctcat
    10621 cgtggaaacg gccctggaga tcggaagata taagccatcg gtcatgattc atccggggcg
    10681 gcattcctcc tggtacggcg acgatgtcca acgatctcgc gccatggcca ttctcggtgc
    10741 cgttttgggc acatgggggc gcgagggcgg catctatctt cccaccaagg catccatgaa
    10801 gaaggtcatc aaggaagatt atccggagcc ccagaagggc cccattgtcg agctgtgcaa
    10861 ataccccttt gccatcaagc agggcctcat cctgggttcc acaaccgcca ttcgggaggc
    10921 aaccctcacg gaacaaccct atcccgtcaa ggggtggatc gtcctggggg ccaacgtcgt
    10981 caaggccatg cccaacgaga aggaaaccct ggaggccatc aataagctgg acctgctctt
    11041 gaccatcgag atcatgcccc tggaaacggc aatggtttcc gatgtcatcc tgccgtcggc
    11101 atcgtacctg gaacaggccg aggagctgga gatcaagaag ggaaggtccc tgtttgtgac
    11161 catgggcagg gcggcggtac cccccatgta cgagagcaag gacgggtata ccattgcccg
    11221 ggagattgcc aagcgaatcg gggtcgggga tttcttcccg tgggaaaccc tggaagaaaa
    11281 gaacaagttc cagtgtcagg ccctcaacct cgactatgaa gagctcagcg aaaagggatg
    11341 catcgtgatc cccaacacgg acagtcctta tatcacgccg gaaaatcagc cggttttcaa
    11401 aactccctcc aagaaaatcg aactgtatag tgctcagctg gcagcaaaag gatttgaccc
    11461 catccccaag tacacccccc cgcagcaacc cccagagggc catttcagac tggtttacgg
    11521 cagaagcccg gtcctttcct tcacgagaac ctgcaacaac ccgatcctca ctgagcttca
    11581 caacgagaac agcatctggg tcaacgccaa gaaggcacga gagttgggca tcaaggaggg
    11641 agagtatgtg gtcctggaaa accaggacgg ggtcaaaagc aacaaaatac gggcaaaggt
    11701 tacccagaga atccgagagg attgcgtgta catggtgcat ggtttcggca gcgaactcaa
    11761 aggcctccgc aacgcttacc gcaagggtgc agacgaccag ggactgatta caaggtacac
    11821 cattgatcct atctccgggt ctacgggact gagagtgaat ttcgtccgga ttctgaagga
    11881 ggcctaaaaa tgccaagata tgccatggtt atagaccaga gaaggtgcgt cggctgtatg
    11941 gcctgcatcg ttgcgtgcaa aaatgaaaac gccgtgccgg cggaacatta taggacgagg
    12001 gtggtggaag tcgtggaggg agaattcccc aatctccgaa tggagttgcg ttcagagctt
    12061 tgcaaccact gcgacaatcc tccttgtgtg tacaattgtc ccaccggagc ttcc
//
LOCUS       DAOOVC010000116        12002 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3483_length_12002_cov_6.731732, whole genome shotgun sequence.
ACCESSION   DAOOVC010000116 DAOOVC010000000
VERSION     DAOOVC010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 12002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12002
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3483_length_12002_cov_6.731732"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1191
                     /locus_tag="PLM79_13245"
     CDS             412..1191
                     /locus_tag="PLM79_13245"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOV87323.1"
                     /translation="MEIPLFQVDAFTKTLFRGNPAAVCPLEYWLHERILQAIAAENNL
                     SETAFFVAESDLYELRWFTPKVEVDLCGHATLAAAHVILNHRDPSASMVRFHTKSGPL
                     TVSREEDRLVMELPSRPGALCPGPSNLVRALGFEPLEVYFSRDYLVVAPKEETVRGLD
                     PDMDLLSKVDGLGVIVTAPGTESDFVSRFFAPKVGVPEDPVTGSAHCTLIPYWSQRLG
                     KKTLHAKQLSLRGGELFCRDEGDRVSIGGHAVTFLEGRIRF"
     gene            1619..2272
                     /locus_tag="PLM79_13250"
     CDS             1619..2272
                     /locus_tag="PLM79_13250"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV87324.1"
                     /translation="MPDAPRGSILQRDKKTYAIVPRTPVGLLSLQNLEALTEVVRKYE
                     IPIVKITSGQRLALVGIKEEDVEGVWKDLGMDMGKATELCVHYVQACPGTAVCRFGVQ
                     DSLGVGLEVEKLFVGMELPAKLKIGVSGCPMTCGESFVRDLGILGKKNGWTFIFGGHS
                     GASARVGDILAEGLDTQQILDLTRRCLNYYRDHAKKKERAAAFVERVGLEEIKKALL"
     gene            2416..3408
                     /locus_tag="PLM79_13255"
     CDS             2416..3408
                     /locus_tag="PLM79_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87325.1"
                     /translation="MDQSLTDFCRSHEIQWNESPRLQLPSRRTWLIEDTKGGRWIAKR
                     TGPDDRNPQLLKGFSVMHPPFHYPRSVADPAEPFLLYPFLEGKVLAEGLFEQSDVVEQ
                     VVEVIGRIQSIMRSLVLVPFYQEILEGRGGAGTGPSMDRYSLGKIMFVDDMQKVSRRK
                     EMAESYQWMQQCTEEYCTSVRSLGIWPDAPLDVYREQVKNTFSIHVPVVGSSLSHTAF
                     HPEHLILCPDEQLGVVGWQVEPRPRFYMTYTYLAWSLLRSRQPAPADYYRRYLERSSS
                     KAFYKDHHQVFACCLLEQACKWFDEKTSSSYRVPDRNLDEAGLLFRECVLNAAK"
     gene            3838..4503
                     /locus_tag="PLM79_13260"
     CDS             3838..4503
                     /locus_tag="PLM79_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOV87326.1"
                     /translation="MVSFDVCIFDVNGVLLDSNAANALAMGRAFTEDPVLRERVARRY
                     LQLTGIDRGKKIRIIQEEIVGRPFEEGEFECRWEGFKVHGRLSMLQAPLGAGSIEVLT
                     ELGKRNIRRAALSNTPLKELMETLEAHDLTRFLDIIRGGGDWPKSESLRRLLDEFSLS
                     PGKCLFLGDGKGDLAAARSNGVPFVAIDAGRGEFHGEEGFDGPFENLAHWGRRNLGIV
                     ERS"
     gene            4541..5104
                     /locus_tag="PLM79_13265"
     CDS             4541..5104
                     /locus_tag="PLM79_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV87327.1"
                     /translation="MPDRENDISLLLAALKFCAERHRMQRRKDADKTPYINHPIEVAE
                     LLWRVGSVRDLQVILAALLHDLLEDTETRPAEIEERFGRDVLLLVLEVSDDKSLPKSQ
                     RKQLQIDHAPRLSLGAKWIKIADKICNVRDVTCSPPANWSRQRRREYLEWTERVVAGV
                     RGAHPVLEALYDEVLQEGRRGTEAEKP"
     gene            complement(5514..6329)
                     /locus_tag="PLM79_13270"
     CDS             complement(5514..6329)
                     /locus_tag="PLM79_13270"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87328.1"
                     /translation="MSSYFVSFDPRMPMEENLPTFSSMEDPRVVGLVKDAAGVFLPTY
                     VTPSRYDSIVRHSRSWFPRFQAKFRYCGKIRQVRLFRKLGVPHPPSLVFRDPEHLRSF
                     FPKRGSPWGYPFVLKGDTGGGGSRVFPVRHPQELEVRLAGLPPDEPVLIQQWVDHGGK
                     DLRVVVYGDAAVSYFRIGGGTFYNNLCRGGREDHASCPELQEMGRFAALDLCARAEID
                     LAGLDLMFPPRSRPVFVEINFHFGRKGLGGTPGHRRHLSEAVRRWRERILRGL"
     gene            complement(6381..6791)
                     /locus_tag="PLM79_13275"
     CDS             complement(6381..6791)
                     /locus_tag="PLM79_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87329.1"
                     /translation="MKKYLFSTLAFLLLLSGTIHAASTAPNVVGTYGGTAKVVCKAGF
                     FDAKVMLKITKQKGHVLKGTASLEDFPPGESTSISFNGVIMGDTIEMIMGTDTHGRAT
                     ISGTSAEGYVHSIGDFGVIGTYKPNSVWFLIKKQ"
     gene            complement(6909..7583)
                     /locus_tag="PLM79_13280"
     CDS             complement(6909..7583)
                     /locus_tag="PLM79_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87330.1"
                     /translation="MAVDLYTLRRKAILILGPKDLRQTVHVLQLAVRNALSQDAQALV
                     DQAVRAAYREWDQASTFPQRVVQVGMDGNPKPGSPIFPWSPARKVFCFDSDLLVPWGY
                     VGERGIHGYSVRSQEEQAALNDSRAGSRVDGTRARVYRGSRAGKAFGAEHDFSGYGRE
                     APPGMKPADPSFGAREEIGQARMGKIVSLIRLIESKGPRASLADREFLHALKQKASQL
                     AHFPLG"
     gene            complement(8004..8933)
                     /locus_tag="PLM79_13285"
     CDS             complement(8004..8933)
                     /locus_tag="PLM79_13285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOV87331.1"
                     /translation="MTTTTPSKLERILAAGHFAVTSECGPPRSADPEVVAHKAQMLAD
                     FVDAINVTDNQTAVVRQCSLASCIRIKQLGLEPVLQMVTRDRNRIALQSDLLGAASFG
                     IHNVLCLTGDHQSFGDHPNAANVFDLDSTQLIQTVKMMRDECKLLSGFEMKDCPKMFI
                     GAAENPFADPFEIRVSRLAKKVAAGAQFIQTQCIYNMEKFKKWMQGVVDRGLDGKVAV
                     LAGLTPMKSVGMAKYMKSRVPGMDVPDDIIKRLAGVPKDKVAEEGIKICVEQIQELKE
                     VKGVRGFHIMAIEWEEKVPEIVKAAGLYPRPQV"
     gene            complement(8930..9598)
                     /locus_tag="PLM79_13290"
     CDS             complement(8930..9598)
                     /locus_tag="PLM79_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV87332.1"
                     /translation="MIIAKPKPIEEIIETTKEFKKILVAGCDGCVTVCEAGGMKEAQI
                     VGSALRIYFKQQGMDVKVDEISLTRQCDRPYVAQLEEKLKDYDAVVSMACGAGVQFLA
                     EAFKDKPILPGVNTCFIGVTEAKGEWTERCQACGECILATTGGICPIARCSKRMSNGP
                     CGGSANGKCEVNKEIACGWHLIYERLKELGQLDRFERPTSPKNWNTSRDGGPRKVVKE
                     VSQL"
     gene            complement(9595..10050)
                     /locus_tag="PLM79_13295"
     CDS             complement(9595..10050)
                     /locus_tag="PLM79_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271121219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(10003..10005),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV87333.1"
                     /translation="MEEFEPIIIAFCCSYUAYSAADLAGSMRLNYPTNVKIVKLPCSG
                     RVDVIHLMKAIEEGADGVFVAGCLEGDCHYQVGNLRAKKRVAYVRGLLEKIGIGGERV
                     AMYNLSAGQGPRFAEIAREMTEKIRKLGPSPLRGAGNQPSPGPKTEVAA"
     gene            complement(10050..>12002)
                     /locus_tag="PLM79_13300"
     CDS             complement(10050..>12002)
                     /locus_tag="PLM79_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87334.1"
                     /translation="EVWLDTVELANRLGIQLNRHRFAVTDGFLPVAASRPGIYVCGTL
                     QGPKDIPQSVMEASGAAAGTSALLSEVRGTQTKTMELPPERDVSGEEPRIGVFVCNCG
                     INIGGIVDVPAVREYARTLPHVIHVEDNLFTCSQDTQVRMGEVIKEKGINRMVVAACT
                     PLTHEALFRETLLSIGLNKYLFEMANIRNQDSWVHMTDHEKATEKAKDLVRMSVARAA
                     LLKGLRDKKVDIRQSGLVIGGGVAGLNAALNLAQQGFDAVLVEKEAELGGLARRISRT
                     IEGREVQPYLDRLIADVRAHQRIRVFTEAQVVSFKGYKGNFTTKIAWGPDQVEETIEH
                     GVAIVATGAHEYRPKEFLYGQDDRVMTQLELDQLLREKGNGRDPSRWRRAVMIQCVGS
                     RNEENPNCSRICCQSAVKHALELKERNPDMDLYILYRDMRMYGLLEDYYTEARSKGIL
                     FSRFDPDRVPEVSADGGNLEVTFVDHVLQRPVRVPADAVILSAATLANETDQLAGQLK
                     VPRNAFGFFIEAHAKLRPVDFSSEGIYLCGTAHAPKLISESITQALAAASRAGSFLAK
                     KSLSVGGAVAHVDKSLCAACLVCVMSCPYNVPRINEENVSEINEALCQGCGVCVSECP
                     AKAIQLSHYEDDQVASNIHALLEGVA"
BASE COUNT         2311 a         3632 c         3648 g         2411 t
ORIGIN      
        1 ggaaacgtag aagaacattt tttgctgccg atgatcgggg gtcctcatga atcattttca
       61 tcttttctac acgatttcaa tgagatgaat ctacatcaaa cctgaagata atgcgcgcat
      121 atttgttgct ttttcaacac cccgttaggg ggccccgtcg caggatcgtc ctcgatcctg
      181 gtcgtccgga ggttcccagg gagcggtgaa aaagcctctg accttgacga gcagattcgc
      241 accggccgga gaagtccgtg ggccgtggag caaatgcgtc gagaatttgg cacaggagat
      301 tgacggggaa atcaagcgca aagagcaccg ggcgcttgca cccaatcccg agcttcaggc
      361 gcaccggcct cagtgggaaa ccctaacctt accattttcg ggaggtcgct catggaaata
      421 cccctctttc aagtggacgc cttcaccaag acccttttcc gaggcaaccc cgccgccgta
      481 tgcccgctgg aatactggct gcacgagcga atcctccagg ccattgccgc cgaaaacaac
      541 ctttccgaga ccgctttttt cgttgccgaa tcggacctct acgaattgcg gtggtttact
      601 cccaaagtgg aggtggacct gtgcggccat gccaccctgg ccgccgctca tgtcattttg
      661 aatcataggg acccgtccgc ttcaatggtg cggtttcaca ccaagagcgg tcccctgacg
      721 gtatcccggg aggaggaccg gctggtcatg gagctccctt cccggccggg cgctctttgc
      781 cctgggccct cgaacctggt gagagccctg ggcttcgagc cgctcgaggt ctatttctcc
      841 cgggattacc tggtggttgc cccgaaggag gagaccgtcc ggggactcga tcccgatatg
      901 gaccttcttt caaaggtcga cgggttgggg gtcatcgtca cggctcccgg cacggaatcg
      961 gatttcgtat ctcggttctt tgcccccaag gtgggagttc ccgaagaccc ggtgaccggg
     1021 tccgcccact gcactctcat cccctactgg tcccaaaggt tgggaaaaaa gactcttcac
     1081 gccaaacagc tctctttgcg gggcggagaa cttttctgcc gagacgaggg agaccgggtg
     1141 agcatcgggg gccacgcggt gaccttcctc gaggggcgga ttcggttttg acgacggggt
     1201 cggtgtgcgg gccgaaagcc cgaagcttct gaatcggatg tggacggcga gcctttatcc
     1261 ctggtataag ggttggatcc aggcaattcc ctgcgacgga tccatgagga ggccgttccc
     1321 tctccccgcg gcccgtccag ggagttgaca acccggggtt ttccttgccg ggagggcaaa
     1381 aagccccctc ccccctgggc gggagatacc ggaggaaggg aaagcttctg cctgccgatc
     1441 agtcctgggt gtgagaacta cggcttccgg aaaccctttc aaggatcttc cacaagtggt
     1501 gtttttgcct tctcgacgat ctatgccgtg aggggtttgt gtcgccgggt tgccccggag
     1561 attccggccc cggggaatcc agcggaataa atgttgcctg tggacgagga gaaaaatgat
     1621 gccggatgct cctagaggtt cgattcttca aagagacaag aaaacttacg ccattgtgcc
     1681 gcgaactccc gtgggattgc tgagcctcca aaacctggaa gccctcaccg aggtggtgcg
     1741 caagtacgaa attcccatcg tcaagatcac gtcggggcag cgactggccc tggtggggat
     1801 caaggaagag gatgtcgagg gagtttggaa agacttgggc atggacatgg gcaaagccac
     1861 ggagctttgc gtacactatg tccaggcatg tcccggaact gccgtgtgcc gcttcggcgt
     1921 ccaggactct ctcggagtgg gtctcgaggt cgagaagctc tttgtgggca tggaacttcc
     1981 ggccaagctc aagatcggtg tgtcgggctg ccctatgacc tgtggggaga gcttcgttcg
     2041 agacctggga atcctgggga aaaagaacgg gtggaccttc atcttcggcg gccattccgg
     2101 ggcaagcgcc cgggtgggag acattctcgc cgaagggctc gatacccaac agatcctgga
     2161 ccttacccgc cgttgcctga attattatcg ggaccatgcc aagaagaagg aacgagctgc
     2221 ggcgttcgtg gaacgggtgg gactcgagga gatcaaaaag gccctcctct agagtccccg
     2281 tggggtccgg ggaaaaggga ccaagacccc gttggggagt tggttgccga aatcttgacc
     2341 tttcctgcac gcgaaacggc ggttccctcc acccctccct ccgccccgaa aggggccgag
     2401 ggcaaggaga atgccatgga ccaatctctc acggacttct gtcgctccca tgaaattcaa
     2461 tggaacgaat ctccccggct ccagcttcct tcccgaagaa cctggctcat tgaagacacc
     2521 aagggcggga ggtggatcgc caagcgcacg gggcccgatg atcggaaccc tcagcttctc
     2581 aaggggtttt ccgtcatgca cccccccttt cattacccgc gatccgttgc ggaccccgcc
     2641 gaaccctttc tgctctaccc cttcctggag gggaaagtcc tggccgaggg gctgtttgaa
     2701 caatcggatg ttgtcgagca ggtcgtggaa gtgatcggca ggatccagtc catcatgcgc
     2761 tccctggttc tggtcccctt ttaccaggaa atcctcgagg gcaggggagg cgcgggaacc
     2821 ggtccgtcca tggaccgcta ttccctgggc aaaatcatgt tcgtggacga catgcagaag
     2881 gtctcccgtc gaaaggaaat ggccgaatcc taccagtgga tgcaacaatg caccgaggag
     2941 tactgcacct ccgtccggtc cctgggtata tggccggacg cacccctgga cgtctaccgg
     3001 gagcaggtga agaacacctt ttccattcac gtcccggtgg tgggcagcag cctttcccat
     3061 acggcctttc atccggaaca cctcatcctc tgccccgacg agcaactggg ggtcgtggga
     3121 tggcaggtgg agccccgacc caggttttac atgacctaca cgtacctggc ctggagcctt
     3181 ttgcgctccc ggcagcccgc cccggcggac tattatcggc gctacctcga gcgaagcagc
     3241 agcaaagcgt tctacaagga ccatcatcag gtctttgcct gttgtttgct cgagcaggca
     3301 tgcaagtggt ttgacgagaa gacatcgagc agctatcggg tgcccgaccg gaacctggat
     3361 gaagccggat tgctgtttcg ggagtgcgtc ctcaacgcgg caaaatgatt tccagttgcg
     3421 gggcaaagcg atgaaccctc ttaagaaatt catggcaacc gcctgcgaac ggtgtcccct
     3481 ttgccgacac gctcgagaga atcccgacac ctggatcggg aaggccatgg aatggcatgg
     3541 gaaatggtgc cccttttgga aggctcacaa agagttcgat cgggagcgcc ggagcaaagg
     3601 gtagggctgt atcacgcagt ccgatggagt gaccgggaat cgtcgccggt tgtgactgcc
     3661 acggaattgc ccgtccggcc atgcgccgga agcggatcgt gattgcggcg caagccggca
     3721 ttcggctgcg cacaattctg cgggttccgg ggaaacccct gtcggaatgt cgaagtgtct
     3781 gcacagaggt cttcctttcc cttcatgaag gtcccctaga aacccaaagg actcggcatg
     3841 gtttctttcg acgtctgcat ctttgacgta aacggggtcc tcctcgattc caacgcggcc
     3901 aacgccctgg ccatggggcg cgctttcacc gaagatcccg tcctgcggga gcgggtggcc
     3961 cgtcggtatc ttcaactgac gggaatcgac cggggcaaaa agatccggat tatccaggaa
     4021 gagatcgtcg gaagaccctt cgaggagggg gaattcgaat gcaggtggga aggctttaag
     4081 gttcatgggc ggctgtccat gctgcaggcc ccgctggggg ccgggagcat cgaagtgctc
     4141 accgaactgg gaaagcgcaa catccgccgg gcagccctct ccaacacccc tttgaaggaa
     4201 ttgatggaaa ccctcgaagc ccatgacctc acgcggttcc tggacatcat ccggggcggg
     4261 ggggactggc ccaagtcgga atccctccgg aggctcctcg acgaattcag cctctctccg
     4321 ggaaaatgcc tctttctggg ggacggaaaa ggggacctgg cggcagcccg ctcgaacggc
     4381 gttcctttcg tcgccatcga cgcgggaagg ggggaattcc acggcgagga gggcttcgac
     4441 ggcccctttg aaaacctggc ccactgggga cggaggaact tgggcatcgt ggaacgatca
     4501 taggtcgacc aaccaacccg ccatcatggg aacagcttcc atgccggacc gtgagaacga
     4561 catctccctg ctgctggcgg ccttgaaatt ctgcgccgag cgccatcgga tgcagcgcag
     4621 aaaggatgcc gacaaaaccc cctacatcaa ccatcccatc gaagtggcgg aactcctgtg
     4681 gcgcgtggga tccgtccggg acctgcaggt catccttgcc gctcttctcc acgacctcct
     4741 cgaggacacg gaaacccgcc ccgcggaaat cgaggaaagg ttcggaaggg acgttctcct
     4801 cctggtcctc gaggtttcgg acgataagag ccttcccaaa tcgcagagaa agcagctcca
     4861 gatcgaccac gctccccgcc tcagccttgg ggccaagtgg atcaagattg cggacaagat
     4921 ctgcaatgtc cgagacgtga catgttcccc acccgccaac tggagccggc aaaggcgccg
     4981 agagtacctc gaatggacgg aaagggtggt ggcgggtgtg cggggcgccc atccggtcct
     5041 cgaagccctc tacgacgagg tgttgcagga aggcaggaga ggaaccgaag ccgaaaagcc
     5101 ctgaggaggc cgggaggttt gccgggacca tcgaaaacat ggcccggatt tccctgtcaa
     5161 gggggctgct ttcttttccc gtttacaccc ctggcctgcc ctttacccac atctgcgatc
     5221 aggtggggat ggatcgacgc ctccgggcga acccgggggg cgcaaccatt agcagggggt
     5281 cgaggccgca aggccgatac ctccggaaca gctcacaaga ggaactcgac ccatggggac
     5341 gcagaaccgt cccgtggaca agaaagggat tgcgccccac acgcctggta ggggcggccg
     5401 gtctccccta tcaccctctg gacgcacggg acaaggccat acggagatct gcccccggag
     5461 cagcaaaaat gtgggagagg ggcagcccta gcctccagcc cccggcccct cgcctaaagc
     5521 ccccggagga ttcgttcccg ccatcgacgg accgcctcgg acaaatgcct tcggtggccc
     5581 ggcgttcccc ccagtccctt tcttccgaag tggaaattga tctcgacaaa caccggtcgc
     5641 gatcgggggg gaaacatgag gtccaatccc gccaggtcga tctccgctct tgcgcacaga
     5701 tcgagggccg caaaacgccc catctcctgg agctcggggc aggaggcgtg gtcctcgcgc
     5761 ccgccccgac acaggttgtt gtagaacgtg ccgcccccga tgcgaaagta gctcaccgcg
     5821 gcatccccgt acaccaccac ccgcaagtcc ttgcccccgt ggtccaccca ctgctggatg
     5881 aggaccggct cgtccggggg gagcccagcc agcctcactt cgagttcttg agggtgacgc
     5941 acgggaaaga cccggcttcc gcctccgccg gtatctcctt tcaacacaaa gggatatccc
     6001 cagggagaac cccgtttcgg gaagaagctc cgaagatgct cggggtcccg gaaaaccagg
     6061 ctgggaggat gagggacccc cagcttccga aaaagacgaa cctgccggat cttgccgcag
     6121 taccggaact tggcttggaa gcggggaaac caggaacggg agtgtcggac gatggagtcg
     6181 taccgcgacg gggtcacgta ggtggggagg aagaccccgg cggcgtcctt cacgagtccg
     6241 acgacccggg ggtcctccat ggaagagaag gtgggcaggt tctcttccat gggcatccgg
     6301 ggatcaaagg aaacgaagta ggaggacatg ggctttcatc cccggggttg gcgacaccgc
     6361 cgttcaagac cggaaggggt tcattgtttc ttgatcagga accatacgga gttgggcttg
     6421 taagtgccga tcactccgaa atcgccgata ctgtgcacat acccttcggc ggaagtgcct
     6481 gagatggtgg ccctcccgtg ggtgtccgtt cccatgatca tctcgatggt gtcccccatg
     6541 atcacgccgt tgaaggagat ggaagtgctt tccccggggg ggaagtcttc tagggatgcg
     6601 gttcctttga gcacatggcc cttttgcttg gtgatcttga gcatcacctt ggcatcgaag
     6661 aaacccgcct tgcacaccac cttggccgtt ccgccgtagg tgcccaccac gttcggggcc
     6721 gtggatgccg catgaatcgt tccgcttagc agtagtagaa aagccagggt cgaaaagaga
     6781 tacttcttca tgaaagatgc ctccgcttga atatggttcc aaaacctcga ttccgacggg
     6841 catccttcta tcatagactc tcccaacagg taagaaaaat ctgaaaacgg accctaccgg
     6901 gacgaccctt atcccagggg gaaatgggcc aactgggagg ccttctgttt caaggcgtgc
     6961 aggaattcgc gatcggcgag ggaggcccga ggtcccttgg actcgatgag ccgtatgagg
     7021 gaaacgatct tgcccatcct tgcctgtccg atctcttccc gggcgccgaa ggaggggtct
     7081 gcgggcttca tgccgggagg cgcctcccgc ccgtatcccg agaagtcgtg ttccgccccg
     7141 aaggcttttc cggcccggga gccccggtag acgcgggccc gagttccatc cacccgggat
     7201 cccgcccggg aatcattcaa ggcagcctgt tcctcctggg agcgaaccga atacccatgg
     7261 attcccctct cccccacata tccccaagga accagcaggt ccgaatcgaa gcaaaacact
     7321 ttgcgggcag gactccaggg gaaaatgggg gatcccggct tggggttccc gtccatgccc
     7381 acctggacca ctcgctgggg gaacgtcgaa gcctgatccc actcccggta ggcggcccgg
     7441 acggcctggt ccaccagggc ctgggcatcc tgggacaggg cgttgcgaac ggccaattga
     7501 agcacgtgga cggtttgacg aagatccttg ggtccaagga tcaggatggc ttttctccgg
     7561 agggtgtaca ggtctacggc catgggtttg cctccccggg aaaggaggat ttcctttccg
     7621 ctcttgcgtg ggttctggaa cattgatccc cgggtgccgg aggactgctt tggggtgcaa
     7681 ggtgaatatt aggtgaaagt cattcggggg tcaaggattc ttttgggagg cacgattttt
     7741 tcggaaagaa aaaacccgga aacggcctcg gggacgttac cgggtttcaa agggtcggcg
     7801 ggaggcgccc gcaggcgtct cgatatcttg cggattcttc ccggaccctc ctcctgtggc
     7861 ctcggcgtcc caaagaaagg cctggagagg ttccggaccc ttcctctttc ttcgacgacc
     7921 cctcccgtca ggaatgggag cctggagacg gaggccttgg gccgaaagag gatgggtttg
     7981 ggtctttcag aaggtcaatc ggttcacacc tggggtctcg ggtacagccc ggcggctttc
     8041 acgatttcag ggaccttctc ttcccattcg atggccatga tgtggaagcc tcgaactccc
     8101 ttgacttcct taagctcctg gatctgctcg acgcaaattt tgatgccctc ttcggccacc
     8161 ttgtccttgg gcacccccgc caatcgcttg atgatatcgt cgggaacgtc catgcccggg
     8221 acccgggact tcatatactt ggccataccg acggatttca tgggagtgag ccccgccaga
     8281 acggccacct tgccgtccag gccccggtcc accacgccct gcatccactt cttgaatttc
     8341 tccatgttgt agatgcactg ggtctggatg aactgggccc ccgccgccac cttcttagcc
     8401 aagcgggata cccggatttc gaagggatcc gcgaaggggt tctcggcggc cccgatgaac
     8461 atcttgggac agtccttcat ctcgaagccg ctcagcagct tgcactcgtc ccgcatcatc
     8521 ttcaccgtct ggatgagctg ggtggagtcg aggtcgaaga cgttggccgc attgggatgg
     8581 tccccgaaac tctgatggtc cccggtgaga cacagcacgt tgtggatgcc gaaggatgcg
     8641 gcacccagga ggtcgctctg gagggcgatg cggtttcggt cccgggtgac catctggagc
     8701 acgggttcga gcccgagctg cttgatgcgg atgcaggagg ccaggctgca ctgtcgaacc
     8761 acggccgtct ggttgtcggt gacgttgatg gcatccacga agtccgcgag catctgggcc
     8821 ttgtgcgcca ccacttcggg gtcggcgctc cggggaggac cgcattcgga ggtcacggcg
     8881 aaatgtcctg cggcaagaat cctttccagt ttgctgggtg ttgtggtggt catagttgtg
     8941 aaacctcctt cacgactttg cggggtccgc catcccggct cgtgttccag ttcttggggc
     9001 tggtgggccg ctcgaagcga tccagttgcc cgagttcctt cagcctttca taaatcaggt
     9061 gccatccgca cgcgatttcc ttgttgactt cgcacttgcc gttggccgat ccgccgcagg
     9121 ggccgttgga catgcgcttg gaacagcggg cgatggggca gatgcccccc gtggtggcca
     9181 aaatgcattc cccgcaggcc tggcaacgct cggtccactc ccccttggct tcggtgacgc
     9241 cgataaaaca ggtattgacc cccggcagga tgggcttgtc cttgaacgcc tcggccagga
     9301 actggacccc ggccccacag gccatggaca cgacggcatc gtagtccttg agcttctctt
     9361 cgagttgagc cacgtaaggc cggtcgcact ggcgggtgag actgatctca tccaccttca
     9421 cgtccatccc ctgctgcttg aagtagatgc ggagggcgga tcccacgatc tgggcttcct
     9481 tcattccccc cgcttcgcaa acggtcacgc agccgtcaca gcctgcgacg agaatcttct
     9541 tgaattcctt ggttgtctcg atgatttcct cgatgggttt cggcttggcg atgatcatgc
     9601 ggctacctcc gttttcggcc cgggggacgg ctggttgccc gctcccctca ggggactcgg
     9661 ccccaatttc ctgattttct ccgtcatttc tcgagcaatt tccgcaaacc tggggccttg
     9721 tccggcggaa aggttataca tggcaacccg ttccccgccg attccaatct tttcgagaag
     9781 tcctcggaca taggccaccc gcttcttggc tcgcagattt cccacctggt agtgacagtc
     9841 cccctcgagg cacccggcca cgaagacgcc gtcggctcct tcctcgatgg ctttcatgag
     9901 gtggatgacg tccacccgac cgctgcaggg gagcttcacg attttcacgt tggtggggta
     9961 gttcaaacgc atggaacctg ccaggtccgc tgcggagtac gctcagtagc tgcagcaaaa
    10021 ggcaatgatg atcggttcga actcttccat cacgcaactc cttccaacaa ggcatgtatg
    10081 ttcgaagcca cctggtcgtc ttcgtagtgg ctcaattgaa tggccttggc cgggcattcc
    10141 gacacgcaga ccccgcagcc ctgacagagg gcttcgttga tctccgatac gttctcctca
    10201 ttgatccgcg gcacattgta cgggcagctc atgacgcaaa ccaggcaggc ggcgcacagg
    10261 gacttgtcca cgtgagccac ggcccctccc acgctgaggc tctttttggc caggaaggac
    10321 ccggcccggg atgccgcggc caacgcctgg gtgatgctct cgctgatgag cttgggcgcg
    10381 tgggccgttc cgcacaggta gatcccctcg gaggagaagt ccacgggccg aagcttggcg
    10441 tgggcttcga tgaagaagcc gaaggcgttt cgcgggacct tgagctggcc ggccagctgg
    10501 tccgtctcgt tggccagggt cgcggcgctc aggatcacgg cgtccgccgg gacccttacc
    10561 gggcgttgga gcacgtggtc cacgaaggtg acctcgaggt tcccgccgtc ggcgctcact
    10621 tccgggaccc ggtccgggtc gaagcggctg aagaggatcc ctttgctccg ggcctcggtg
    10681 tagtaatcct cgagcaaccc gtacattctc atgtctcgat agagaatgta gaggtccatg
    10741 tcgggattgc gttctttcag ctcgagggca tgcttcacgg cgctctggca acagatccgg
    10801 ctgcagttgg gattctcttc gttgcgggac cccacgcatt ggatcatgac cgctcgtcgc
    10861 caccgggacg gatcccggcc gttgcccttc tcccgcagga gctgatccaa ttcgagctgg
    10921 gtcatcaccc ggtcgtcctg accgtagaga aattctttgg gccggtattc gtgggccccc
    10981 gtggccacaa tggccacccc gtgctcgatg gtttcttcca cctggtcggg tccccaggcg
    11041 atcttggtgg tgaagttgcc cttgtagcct ttgaaagaga ccacctgggc ctcggtaaaa
    11101 acccggattc tttggtgggc tcgaacgtcg gctatgagcc ggtccaggta gggctgcacc
    11161 tcgcggcctt cgatggtgcg ggagatcctg cgggccaggc cccccaactc cgcctccttt
    11221 tccaccagga cggcatcgaa gccctgctga gccaggttca gggccgcatt caacccggcg
    11281 acgccccctc cgatgaccag gccgctctgc cggatgtcca ccttcttgtc ccggagccct
    11341 ttgagcagcg ccgcccgggc cacggacatg cgcaccaggt ccttggcctt ttccgtggcc
    11401 ttttcatggt ccgtcatgtg gacccaggag tcctgattgc ggatattggc catctcgaaa
    11461 aggtacttgt tgaggccgat gctgagaagg gtctcccgga agagggcttc gtgggtgagg
    11521 ggggtgcagg ccgccaccac catccggttg atccccttct ccttgatgac ttcccccatg
    11581 cgcacctggg tgtcctggga gcaggtgaaa aggttgtcct ccacgtggat cacgtgggga
    11641 agggttctgg cgtactcgcg cacggcggga acatccacaa tcccgccgat gttgatgccg
    11701 caattgcaca cgaacacccc gatccggggc tcttccccgc tcacgtcccg ctcggggggg
    11761 agctccatgg tcttggtttg ggtcccccgg acttcactca gcaacgcgct ggtgcctgcg
    11821 gcggccccgg aagcctccat caccgactgg ggaatgtcct tgggtccctg gagagtcccg
    11881 cagacataga tcccgggccg ggaagcggcc acgggcagga aaccgtcggt gacggcgaaa
    11941 cgatgccgat tcagctggat tcccaggcgg tttgccagtt ccaccgtgtc cagccacacc
    12001 tc
//
LOCUS       DAOOVC010000117        11952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3500_length_11952_cov_7.393461, whole genome shotgun sequence.
ACCESSION   DAOOVC010000117 DAOOVC010000000
VERSION     DAOOVC010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11952
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3500_length_11952_cov_7.393461"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..433
                     /gene="rpsJ"
                     /locus_tag="PLM79_13305"
     CDS             125..433
                     /gene="rpsJ"
                     /locus_tag="PLM79_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOV87335.1"
                     /translation="MTNQRIRIRLKAYDHKLLDQSTNEIVSTAKKTGARVAGPIPLPT
                     KIQRYTVLRSPHVDKKSREQFEIRIHKRLIDILDPTQQTVDSLMKLDLSAGVDVEIKL
                     "
     gene            480..1109
                     /gene="rplC"
                     /locus_tag="PLM79_13310"
     CDS             480..1109
                     /gene="rplC"
                     /locus_tag="PLM79_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOV87336.1"
                     /translation="MKTLIGRKLDMTQVFGEDGSVVPITLIEAGPCTVTQVKTPGTDG
                     YSALQIGFGSRKPKNVNRPTKGHMDKAGKGYFRVLREVRVEDTAGFELGQEISVEAFE
                     VGERIDVVGKSKGKGFAGPIKRWGFQRGPAGHGSKNIREPGSTGNATFPGRVIKGKKM
                     AGQKGNKRVTVMNLQIVDVRPEMNLLLVKGAVPGAKNGIVLIRKTNRGK"
     gene            1150..1773
                     /gene="rplD"
                     /locus_tag="PLM79_13315"
     CDS             1150..1773
                     /gene="rplD"
                     /locus_tag="PLM79_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOV87337.1"
                     /translation="MPTVDVYSINRDVVGQLDLRDDVFGVSVKPHVMHQVVLYQLNRR
                     RAGTAKTKGRSEVAGGGKKPWRQKGTGRARSGTSRSPLWRGGGIIHGPQPRSYEMKVP
                     KKVRALGLRMALSQKLADGELTVLDQLQMERIKTRDFCAILQRFDLAKTLVVIPWRDE
                     LVEKSARNLPNVKVLRSEGLNVYDVLNHHHLLLTRETVGKIEEALGS"
     gene            1770..2063
                     /locus_tag="PLM79_13320"
     CDS             1770..2063
                     /locus_tag="PLM79_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOV87338.1"
                     /translation="MNEKTYRILKRPLVTEKSTTEKEAHNKLYFEVDRRANKIEIRQA
                     VERIFKVNVLEVATMNMKGKRKRAGRFFTKKPDWKKAIVTIKPGQRVEFFEGV"
     gene            2081..2902
                     /gene="rplB"
                     /locus_tag="PLM79_13325"
     CDS             2081..2902
                     /gene="rplB"
                     /locus_tag="PLM79_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOV87339.1"
                     /translation="MSNRTVNPTSPGRRFLTTPKFDEITRREPEKALLEPMKKSGGRN
                     NRGRITAWHRGGGHKRRYRIIDFKRNKENIPAKVASIEYDPNRSAHIALLHYADGEKR
                     YILAPIGIAVGDTVLTSQDADIKPGNCLKLANIPLGTVVHNVEMKPGKGGQLARSAGS
                     GAQLIAKEGRHAILRLPSSEMRMVPIECKATVGQVGNTDHENRSLGKAGRTRWYGRKP
                     HVRGVAMNPVDHPMGGGEGRSSGGRHPCTPWGVPTKGYRTRKGKSSDKMIIRRRK"
     gene            2965..3252
                     /gene="rpsS"
                     /locus_tag="PLM79_13330"
     CDS             2965..3252
                     /gene="rpsS"
                     /locus_tag="PLM79_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOV87340.1"
                     /translation="MPRSLRKGPFVDGHLMEKVRRARDTGDRKVIKTWSRRSTIVPEF
                     VGVTLAVHNGKKFIPVFVTENMVGHKLGEFSPTRTFYGHAGDKKSRVKGKK"
     gene            3252..3584
                     /gene="rplV"
                     /locus_tag="PLM79_13335"
     CDS             3252..3584
                     /gene="rplV"
                     /locus_tag="PLM79_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOV87341.1"
                     /translation="MEVRAVSKYLRVSPHKARLVADLVRGKMVADALTTLKFTPKKSA
                     RFINKTLRSAVANAENARTVDVDTLYVKVILVDEGPRLKRWRPRAMGRATRILKRTSH
                     ITVVLAEK"
     gene            3616..4242
                     /gene="rpsC"
                     /locus_tag="PLM79_13340"
     CDS             3616..4242
                     /gene="rpsC"
                     /locus_tag="PLM79_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041442082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOV87342.1"
                     /translation="MGQKVHPIGFRLGVIQTWDSKWFATRDYPKLVYEDRLIRDYIKA
                     RLSHAGIARVEVERAANKAKIRIHTARPGIVIGKKGAEIEALRQDLERKFKREILIDI
                     QEVRRPELDATLVAENVALQLVRRVAFRRAMKRAVTAALKFGAKGVRVACAGRLGGAE
                     MARREWYREGRVPLHTLRADIDYGTALAKTTYGIIGVKVWIFKGEVLP"
     gene            4380..4796
                     /gene="rplP"
                     /locus_tag="PLM79_13345"
     CDS             4380..4796
                     /gene="rplP"
                     /locus_tag="PLM79_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOV87343.1"
                     /translation="MLAPKRVKYRKQQKGRMRGTAYRGSTLSFGDFGLRAEQCGRISS
                     RQIEAARIAITRHVKRGGKVWIRFFPDKPISKKPAETRMGKGKGSPEAWVAVVKPGRM
                     LYEIKGVPELVAREALRLAAHKLPIPTRFVSRQDIL"
     gene            4793..4993
                     /gene="rpmC"
                     /locus_tag="PLM79_13350"
     CDS             4793..4993
                     /gene="rpmC"
                     /locus_tag="PLM79_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOV87344.1"
                     /translation="MKASALRELTKDELVHKLTELREALFNLQFQHATGQLENTAQLY
                     KTKRTIARILSVLTETERKTAA"
     gene            5005..5268
                     /gene="rpsQ"
                     /locus_tag="PLM79_13355"
     CDS             5005..5268
                     /gene="rpsQ"
                     /locus_tag="PLM79_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOV87345.1"
                     /translation="MENLKSNRKTRIGTVLSNKMDRTVVVQVERLIHHPQYHKLIRRR
                     NKFKAHDGDNSCGVGDRVLIQESRPLSKTKRWVVVTILEKASI"
     gene            5342..5710
                     /gene="rplN"
                     /locus_tag="PLM79_13360"
     CDS             5342..5710
                     /gene="rplN"
                     /locus_tag="PLM79_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOV87346.1"
                     /translation="MIQAETQLNAADNSGAKRLYCIKVLGGTRKRYASVGDIIVVSVK
                     EAIPNAKVKKGDVLKAVVVRTRKEVRRPDGSYIRFDDNSAVLINAAKEPIGTRIFGPV
                     ARELRGKQFMKIISLAPEVL"
     gene            5731..6060
                     /gene="rplX"
                     /locus_tag="PLM79_13365"
     CDS             5731..6060
                     /gene="rplX"
                     /locus_tag="PLM79_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOV87347.1"
                     /translation="MEAGSKYRIKKNDMVMVIAGKEKGKTGKVLRVIPKKDRVIVEKL
                     NMVKRHMKPGPQSRQGGILEREAPIHISNLKLICSKCTDPTRVGYRTLEDDRKVRYCK
                     KCSEVID"
     gene            6103..6642
                     /gene="rplE"
                     /locus_tag="PLM79_13370"
     CDS             6103..6642
                     /gene="rplE"
                     /locus_tag="PLM79_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOV87348.1"
                     /translation="MARLRERYQTEIAPKLREKYQYKSPMEIPRITKVVLNIGLGEAI
                     QNIKILDSAAEELGQISGQKPVITRARLSIAAFKLRKGMPIGCMVTLRGNRMYEFLDK
                     LVNVALPRVRDFRGVSPKSLDGRGNYTLGIKEHIIFPEIDYDKIDRIKGMNITIVTTA
                     ATDDEARSLLSLMGMPFRQ"
     gene            6662..6847
                     /locus_tag="PLM79_13375"
     CDS             6662..6847
                     /locus_tag="PLM79_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOV87349.1"
                     /translation="MAKKSLIAKAKRTPKFSVRKYNRCPVCGRPRAYLRKFGICRICF
                     RNMALQGELPGVVKSSW"
     gene            6879..7277
                     /gene="rpsH"
                     /locus_tag="PLM79_13380"
     CDS             6879..7277
                     /gene="rpsH"
                     /locus_tag="PLM79_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOV87350.1"
                     /translation="MAVTDPIADFLNRIKNGQKARFDKVDIPASQMKISIARILKEEG
                     YIKNYKLIRDNKQGILRVHLKYSDIRQAAIADTKRISRPGCRVYVGHSEIPRVRNGLG
                     ISILSTSKGIMTDHQARQEGIGGELLCTIW"
     gene            7342..7884
                     /gene="rplF"
                     /locus_tag="PLM79_13385"
     CDS             7342..7884
                     /gene="rplF"
                     /locus_tag="PLM79_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOV87351.1"
                     /translation="MSRVGKLPIKVPSGVAVAQENDMITVTGPKGTLSCRLPQQLELE
                     IEPEVVRIKRQDDASPTRGLHGLMRSLVYNMVHGVSQGFTVVLEIQGTGYRAEVQNNV
                     LNLSLGYSHPVQFQVPESIKASVERQTVIRLDGIHKELLGQTAARIRALRPAEPYKGK
                     GIRFAGEHIHRKVGKAGSKK"
     gene            7903..8274
                     /gene="rplR"
                     /locus_tag="PLM79_13390"
     CDS             7903..8274
                     /gene="rplR"
                     /locus_tag="PLM79_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOV87352.1"
                     /translation="MVTKANPREIARQRRKKRIRKKIAGTAERPRLSVFRSDKHIYAQ
                     VIDDTRGQTLVAASTLSPEYREASSSKGKVEAAKKVGELVAVKALEKGISSVVFDRNG
                     FIYHGRVRALADAARQAGLNF"
     gene            8307..8801
                     /gene="rpsE"
                     /locus_tag="PLM79_13395"
     CDS             8307..8801
                     /gene="rpsE"
                     /locus_tag="PLM79_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOV87353.1"
                     /translation="MLAVESSQLQMIDKVVHISRVAKVVKGGRRFSFSAIVVVGDGGG
                     RVGFSLGKANEVPEAIRKGMESAKRMMFEVPLVNGTIPHEVLGRFGASSVVLKPASPG
                     TGVIAGGAVRAIMEAAGIHDILTKCIGSRNPHNVVKAAMEGLRSLRSRDAVSKVRGRE
                     LAQK"
     gene            8949..9131
                     /gene="rpmD"
                     /locus_tag="PLM79_13400"
     CDS             8949..9131
                     /gene="rpmD"
                     /locus_tag="PLM79_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOV87354.1"
                     /translation="MATPIQVKLVRSPIGHPEKHRKVLQALGLTRVNKTVNLYATPTI
                     LGAVRKVIHMVEVKER"
     gene            9236..9694
                     /gene="rplO"
                     /locus_tag="PLM79_13405"
     CDS             9236..9694
                     /gene="rplO"
                     /locus_tag="PLM79_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOV87355.1"
                     /translation="MRLEDLAPAKGAKHSKKRVGRGTGSGLGKTAGRGSKGQKSRSGG
                     GTPPWFEGGQMPLQRRVPKRGFTNIFAARFSILNVKDLQRFEPAGTVDIPDLIRAGYL
                     KKGSAGIKLLGEGDISFSIHVRVHKASPSAISKVEAAGGRVELISLPKRT"
     gene            9698..11005
                     /gene="secY"
                     /locus_tag="PLM79_13410"
     CDS             9698..11005
                     /gene="secY"
                     /locus_tag="PLM79_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOV87356.1"
                     /translation="MLTGFQNIGKIPELKRRIINTLLLLAVYRIGAHIPTPGINTEAL
                     AAFFRAAQGTLLGLFDMFSGGALSQLSVFALGIMPYISSSIILQLLTVVIPHLERLSK
                     EGEAGRRKITQYTRYGTVILSIIQGFGIAFGVEGMTGPGGEMVVLYPGWGFRIITVIT
                     LTAGTAFIMWLGEQITERGIGNGISLIIFAGIVAGIPNATISSFRLLSAGEISLFVAL
                     LLVIMMVVVIGLIIFMERGQRRIPVQYAKRVVGRRVYGGQNTHIPLKINTSGVIPPIF
                     ASSIIMFPATVANFIKVPWVQSVADAFSPGHWLYSVLYVGFIVFFCFFYTAIVFNPID
                     VADNMKKYGGFIPGIRPGRPTAEYIDKVLSRITLGGAIYVSAVCVLPTLLIRKFNVPF
                     YFGGTALLIVIGVAMDTLAQIEAHMLSRHYEGFLKKGRIRGRR"
     gene            11100..11741
                     /locus_tag="PLM79_13415"
     CDS             11100..11741
                     /locus_tag="PLM79_13415"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOV87357.1"
                     /translation="MNIILLGPPGAGKGTQAKRMIDRYHIPQISTGDMLRAALKEGTA
                     LGLEAKKYMDQGALVPDTVVVGLVRERIQKDDCSQGFMLDGFPRNVSQAESLDGMLLE
                     LGKKIDKVVCIEVPSSELLARLTGRRTCRACGAGFHVMFDPPKKDKVCDKCSGELYQR
                     DDDNEATVSSRLQVYEAQTKPLIDYYEKQAKLLRINGLGSIQEIFGRVVSALG"
     gene            11753..>11952
                     /locus_tag="PLM79_13420"
     CDS             11753..>11952
                     /locus_tag="PLM79_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOV87358.1"
                     /translation="MIILRSAREIEKIRKACLVVAEILERLREHIRPNITTWDLNVLS
                     EELAARKKAKPAFKGYHGYPHSL"
BASE COUNT         2797 a         3260 c         3729 g         2166 t
ORIGIN      
        1 ccgggaaggc ggacgcaccg tcggcgccgg ggtcattacc gagatcattc agtaaggaaa
       61 ggcaaggatt ttgccatccg aagaggtccc ttgggggacc ccccaggaat aggaatcgac
      121 gaccatgaca aatcaaagaa ttcgcatccg cctcaaagcg tacgatcata agctcttgga
      181 tcagtccacc aatgaaatcg tgtcgacggc caagaagacg ggagcgaggg tggccggccc
      241 catccctctc cccaccaaga tccagcgcta caccgtgctt cggtcgcccc acgtggacaa
      301 gaagtcgcga gagcagttcg agatccgcat ccacaaacgc ctgatcgaca tactcgatcc
      361 gacccagcag acggtggatt ctttgatgaa gctggacctg tctgcggggg tggacgtgga
      421 gatcaagctt taggacctct caaccgtctg gtttgagcag aaaagagtac gttgccatga
      481 tcaagacact tatcggacgc aaattggaca tgacccaggt cttcggcgag gacggcagtg
      541 tggtgcccat cacgctcata gaggccggac cctgcaccgt cacccaagtc aagacccccg
      601 ggaccgatgg ctactccgct ctccagatcg ggttcggcag ccgaaagccc aagaatgtga
      661 accgaccgac caagggacac atggacaagg cgggcaaagg gtacttccgg gtgctgcggg
      721 aggtgcgcgt ggaggacacg gccggttttg agctgggaca agagatatcc gtggaagcct
      781 ttgaagtcgg cgagcggatc gacgtcgtgg gcaagagcaa agggaagggc tttgccggac
      841 ccatcaagcg ctggggattt cagcggggac cggccggcca tgggtccaag aacatccgtg
      901 agcccggctc taccgggaac gccacctttc ccggtcgtgt catcaagggc aagaagatgg
      961 cgggccagaa agggaacaag cgggtcacgg tcatgaacct tcagattgtg gatgtgaggc
     1021 ccgagatgaa tttgcttctg gtcaaagggg cggtccccgg ggccaagaac ggaatcgtgc
     1081 tcattcggaa gaccaatcgg ggcaaataac tcaggaatcg agaggcctgc gggagattcg
     1141 aggaatacca tgccgactgt ggatgtttat agcataaatc gggacgtggt cgggcaactg
     1201 gatctgcgcg acgatgtttt tggcgtgtcc gtaaagcccc atgtgatgca tcaggtggtg
     1261 ctctaccagc tgaacaggcg tcgagccggg actgccaaga ccaagggcag aagcgaggtg
     1321 gccggcgggg gcaagaagcc gtggcgccag aagggtacgg gacgcgcccg ttccggaacg
     1381 tctcggtcgc cgctgtggcg agggggcggc atcatccacg gaccccagcc cagaagctac
     1441 gagatgaagg tccccaagaa agtgcgggcc ctggggctga gaatggccct cagccaaaag
     1501 ctcgccgacg gggaactcac cgttttggac cagctgcaga tggaacgcat caagaccagg
     1561 gatttttgcg ccatccttca gcgcttcgac ctggccaaga ccctggtggt cattccgtgg
     1621 cgggatgaac tggtcgagaa atcggctcgc aatcttccca atgtgaaggt cctgcgatcc
     1681 gagggactca acgtctacga cgtgctcaac caccaccacc tgcttctaac ccgggagacg
     1741 gttggaaaga tagaggaggc attggggtca tgaacgaaaa gacttaccgg atactcaagc
     1801 gtcccttggt gacggagaag agcaccacgg aaaaggaagc gcataacaag ctgtacttcg
     1861 aagtggaccg cagggcaaac aaaatcgaaa tccgccaggc ggtggagcgc atcttcaagg
     1921 tgaacgtcct ggaggtggcc accatgaaca tgaagggcaa acggaaacgg gcgggccgct
     1981 tcttcaccaa gaagcctgat tggaagaaag cgatcgtcac catcaagccc gggcagcgag
     2041 ttgagttttt cgagggcgtg taagacggga gagaacgagc atgagcaata ggacagtcaa
     2101 cccgacatcc ccggggcgcc ggtttctgac gactcccaag ttcgacgaga tcacccgtcg
     2161 ggagcctgaa aaggccctct tggaacccat gaagaagtcc ggtggacgca acaaccgagg
     2221 ccggatcacg gcatggcacc ggggaggcgg tcacaagcgg cggtaccgga tcatcgattt
     2281 taagaggaac aaggagaaca ttccggccaa ggtggccagc atcgaatacg atcccaaccg
     2341 ttccgcgcac atcgccctgc ttcactatgc ggacggggag aagcgttaca tcctggctcc
     2401 catcgggatt gccgtcgggg ataccgttct cacgagccag gacgccgaca tcaagccggg
     2461 aaactgcctc aagctggcca acattcccct gggtaccgtg gtccacaacg tggagatgaa
     2521 gcccgggaag ggagggcaac tggcccggtc cgccggatcg ggagctcagt tgattgccaa
     2581 ggaaggtcgg cacgccatcc tgcggttgcc ttccagcgag atgcgcatgg tacccatcga
     2641 atgcaaagcc acggtcggcc aagtgggcaa tacggaccac gaaaaccgtt ccctgggcaa
     2701 agcgggaagg acccgctggt acggtcgaaa gcctcacgtt cgaggcgtgg ccatgaaccc
     2761 ggtggatcat cccatgggcg gcggggaagg caggagctcc ggtggacggc atccctgcac
     2821 gccgtggggg gtcccgacca aggggtaccg gacacgcaaa gggaaatcca gcgacaagat
     2881 gatcattcgt cgacgcaaat agcctcatag cccggtcccc ccccggaaaa ggggaccggg
     2941 aggcaaaacg cacaggagga taaggtgcct cgctcgctaa gaaaagggcc gtttgtggat
     3001 ggccacctca tggaaaaagt tcgcagggcc agggataccg gggaccgcaa ggtcatcaag
     3061 acctggtccc gaaggtcgac catcgttccc gagttcgtgg gagtcaccct ggccgtccat
     3121 aacggcaaga agttcatccc ggttttcgtc acggagaaca tggtgggaca taagctgggg
     3181 gaattttcgc ccacccggac tttttacggc cacgcagggg ataaaaaatc cagagtgaag
     3241 gggaagaagt aatggaagtg agagcggtta gcaagtactt gcgcgtttct cctcacaagg
     3301 ctcgactggt tgcggatttg gtgcggggca agatggttgc ggacgcgttg acgactctca
     3361 agttcacacc caagaagagt gcccggttca tcaacaagac cttgcgctcg gccgtggcca
     3421 acgccgaaaa tgcccgaacc gtggatgtgg ataccctgta tgtgaaggtc atccttgtgg
     3481 acgagggacc gcggctcaag cgctggcgcc cacgggccat ggggcgagcc actcgaatcc
     3541 tcaagcgtac gagtcacatc accgttgtgc tggcggagaa ataaccttaa cgattgaaga
     3601 tggtggaggt ggagtttggg acagaaagta catccaattg ggttccggct gggtgtcatc
     3661 caaacctggg attcgaaatg gttcgccacc cgtgattatc ccaagctggt ctatgaagac
     3721 cggctgattc gggattacat caaggcccga ttgtcccacg ccggcatcgc ccgggtggaa
     3781 gtcgaacggg cggccaacaa ggcaaaaatc cgcattcata cggcaagacc cggaatcgta
     3841 attggcaaaa agggggcgga aatcgaagcc ctgcgccagg acctggagag gaaattcaaa
     3901 cgggagatcc ttatcgacat ccaggaagtg cgacgtcccg aactcgacgc caccttggtg
     3961 gctgagaacg tggcccttca actggtgcgc cgcgtggcct ttcgccgggc catgaagcgg
     4021 gcggtgacgg cggcgctcaa gttcggggcc aagggagtcc gggtggcctg cgccgggcgg
     4081 ttggggggag ccgagatggc gaggagggaa tggtaccggg aaggaagagt gcccctccat
     4141 accttgaggg ccgatatcga ttacggcacg gctctggcca agaccaccta cggcatcatc
     4201 ggcgtgaagg tctggatctt caagggggag gttcttccct gagagggatg tgccttgcgg
     4261 caagggcgac cggggaacgc cgaacccagt gggatcgggt tccggggggg ccttgccgtc
     4321 gttgcctgcg tgagattatg aacggggcag gttgcagttg agagacagga gacattccga
     4381 tgttagcgcc caagcgggtt aaatatcgaa agcagcagaa gggccggatg cggggcaccg
     4441 cctaccgggg cagcactctg agttttggcg atttcggcct gagggcggag caatgcgggc
     4501 gaatcagctc ccgccaaatc gaggcggccc gtattgccat cactcgccac gtgaagcggg
     4561 gaggaaaggt gtggattcga ttctttcccg acaagcccat ctccaagaaa ccggcggaaa
     4621 ctcgaatggg aaaaggcaag ggctctcccg aggcttgggt tgccgtggtc aagcccggac
     4681 gaatgctcta cgagatcaag ggggttcccg aactggtggc cagggaagcc ctccgcctgg
     4741 cggcacacaa acttcccatt cccactcggt ttgtatctag acaggatatc ctatgaaagc
     4801 gagcgccctt cgtgaattga caaaagacga gctggtccac aagctcacgg agcttcgcga
     4861 ggccctgttc aacctgcagt tccagcatgc aacgggccag ctggaaaaca ccgcgcagct
     4921 ctacaagacc aagagaacca ttgcgcggat cctctccgtc ttgactgaaa cggaacgaaa
     4981 gaccgcggca taaggcgatg aaagatggaa aatctcaagt ccaataggaa gacaagaatt
     5041 gggacggttt tgagcaacaa gatggatcgg acggtggtgg tgcaggtcga gcgtctcatc
     5101 catcatcccc aataccacaa gctcatccgc aggcgaaata agttcaaggc ccacgacggg
     5161 gacaattcct gcggtgtggg cgatcgggtg ctgatccagg aaagccgccc cctgagcaag
     5221 accaaacggt gggttgtggt gacgattctc gaaaaggctt ccatttaggc cagaggctgt
     5281 tgccggtcgc catggtcggg ttcccggcag ggacggcagc aatgggagga cggagtaggt
     5341 catgattcaa gcagaaactc agctcaatgc cgcggacaac tcgggggcaa agcgcctcta
     5401 ttgcatcaag gtcctggggg gcacgcgcaa acggtatgcc agcgtgggcg acatcatcgt
     5461 cgtatcggtc aaggaagcca ttcccaatgc caaggtcaag aagggggatg tcctgaaggc
     5521 ggtggtcgtg cgcaccagga aggaagtacg ccgtcccgac gggtcctaca tccggttcga
     5581 cgacaattcc gccgtcctca tcaatgcggc gaaggaaccc attgggactc ggatctttgg
     5641 gcccgtagcc agggaactgc gaggaaagca atttatgaag atcatctcat tggcccccga
     5701 agtactctag cgggatgaag gacgtattcc atggaagccg gcagcaagta tcgcattaag
     5761 aagaacgaca tggtgatggt cattgccggg aaggaaaagg gcaagaccgg caaggtgctt
     5821 cgcgtgattc ccaaaaagga ccgagtcatc gttgagaagc tcaacatggt caagcggcac
     5881 atgaaacccg gcccccaaag ccgtcagggc ggtatattgg agcgggaagc gcccattcac
     5941 atctcaaacc tcaaattgat ctgttccaag tgtaccgacc cgacccgagt gggctatcgc
     6001 actctggaag acgaccgcaa agttcgttac tgcaagaaat gcagcgaggt catcgactaa
     6061 gagtcacgtc caccgcctat ggtgcgggag tggaggcaag gtatggcgcg gttgcgtgaa
     6121 aggtatcaaa cggagatcgc tccgaagctg agggaaaagt accaatacaa gagtcccatg
     6181 gagattccga ggatcaccaa ggtcgtgttg aacatcggcc tgggagaggc catccagaat
     6241 atcaagatcc tggattcggc agccgaggag ctgggccaaa tcagcgggca aaagccggtc
     6301 attactcggg cacgtctgag catcgccgcc ttcaagcttc ggaagggcat gcccatcggc
     6361 tgcatggtga cattgcgggg aaaccgcatg tatgaattcc tcgacaaact ggtcaatgtg
     6421 gcgctgcctc gggttcgaga cttccgaggc gtgtcgccca agtccctgga cggtcggggg
     6481 aactacaccc tgggaatcaa ggagcacatc atctttcccg aaatcgatta cgataagatc
     6541 gataggatta aaggcatgaa catcaccatc gtcaccaccg ccgcaacgga cgatgaggct
     6601 cggtcccttt tgagcctgat gggaatgcct ttcaggcagt aggctgaaga aggagactac
     6661 tttggcaaag aaatccttga ttgcaaaagc gaaacggacc cccaagttca gtgtcaggaa
     6721 atacaaccgc tgccccgtat gcggacgacc ccgggcttac ttgcgcaagt tcggcatctg
     6781 ccgcatctgc ttccggaata tggcgctgca gggggagctt ccgggcgtgg tcaaatccag
     6841 ctggtaggct ggctggggag tcagaaggag tatctggtat ggccgtaacg gatcccatcg
     6901 ccgatttcct caatcggatc aagaatggac aaaaggcacg attcgacaag gtggatatcc
     6961 ccgcctccca aatgaagatc agcattgcgc ggatcctgaa agaggaagga tacatcaaaa
     7021 actacaagct gattcgggac aacaagcagg gcattcttcg ggtccacctc aagtacagcg
     7081 acattcggca ggccgccatt gcggacacta agcgcatcag caggccgggc tgccgggtgt
     7141 acgtgggaca ctcggagatt ccccgggtaa ggaacggctt gggtatcagc attctatcca
     7201 cctccaaagg gatcatgacc gatcaccagg cgaggcagga agggatcggc ggggagttgc
     7261 tctgcaccat ctggtagcag gcggcctttt tcgtcctcca acctgtggaa ctacggagca
     7321 tcaggctcaa ggagtaacag catgtcacgt gtgggaaagt tgcccatcaa agtcccctcg
     7381 ggagtcgcgg tggcgcagga aaacgacatg attaccgtaa cggggcccaa agggaccctt
     7441 tcttgccgcc ttccgcagca gttggagttg gagatcgagc cggaggtggt gaggatcaaa
     7501 cggcaggacg acgccagtcc cactcgtggg ttgcatggac tcatgcggag tctggtctac
     7561 aacatggtgc acggtgtgag tcaagggttc acggtggtcc tcgagatcca ggggaccgga
     7621 tatcgggccg aggtgcaaaa caacgtattg aacctcagcc tgggttattc ccacccggtg
     7681 cagttccagg tgccggaatc catcaaggct tccgtggaac gtcagacggt gatccgcctg
     7741 gatggaatcc acaaggagct tttgggccag accgccgccc ggattcgtgc cctccgaccg
     7801 gcggagccgt acaagggaaa agggattcgc ttcgccgggg aacacattca ccgcaaagtg
     7861 ggcaaggctg gttcgaagaa gtaggaacga agggaaagca caatggtcac gaaggcaaac
     7921 ccgcgtgaga ttgcacgtca gagacgaaag aagcgtatcc gaaaaaagat tgccggcacg
     7981 gccgagcggc cccggttgtc ggtgtttcga agcgacaagc acatatacgc ccaggtgatc
     8041 gacgacacca gggggcagac cctggtggcc gcctccaccc tttcaccgga atatcgcgag
     8101 gcgtcctcct ccaaggggaa agtggaagcc gccaagaagg tcggggagtt ggtcgccgtc
     8161 aaggccctcg agaagggcat cagcagcgtc gttttcgatc gaaacgggtt catctaccac
     8221 ggccgtgtgc gggccctggc cgatgcggct cggcaggcag ggcttaactt ctaaggatgg
     8281 ggatcttctc atagggaggc atgcaattgc tagcagtgga gtcaagccaa cttcaaatga
     8341 tcgacaaggt ggtccatatc agtcgggtcg ccaaggttgt caaaggtgga cgtcgctttt
     8401 ccttcagcgc catcgtggtg gtgggggacg gcggcggacg cgtcgggttc agccttggaa
     8461 aagccaacga agtacccgaa gccatccgca agggaatgga atcagccaaa cgaatgatgt
     8521 ttgaagtgcc ccttgtcaac gggacgattc ctcacgaggt cctgggtcgc tttggtgcgt
     8581 cctccgtcgt gttgaaaccc gcctcccccg gtacgggggt cattgcgggg ggcgcggtgc
     8641 gcgccatcat ggaagcggcc ggcatccacg acatcctgac caagtgcatc ggatccagaa
     8701 atccacacaa cgtcgtcaag gcagccatgg aaggtctgag gtccttgagg agccgagacg
     8761 ccgtctccaa ggttcgtggg cgggagctcg cacagaagta atctttcggt ccgtcccggg
     8821 gatctcgatc cacgatccgg cgcgggaggg accgccccgg gaagtccggg ggcctgcgcg
     8881 ggacgagtcc aatattccgg gattcccgga acaacccaag gcgacctagg atattgaagg
     8941 aagagacgat ggccaccccc atacaagtca aactcgttcg aagccccatc gggcacccgg
     9001 agaagcaccg caaggtcctt caggcattgg gcctcaccag ggtcaacaag acggtgaacc
     9061 tgtatgccac ccctaccatc ctgggggccg tgcgcaaggt gatccatatg gtggaagtca
     9121 aagagcggta accgccgatc atgtcgcacc ggggacacgt tccgggtgca cggtttcctc
     9181 cattggacgg caattcgttg cctggaatct actccaaacc atcatggatg tatcaatgag
     9241 actggaagat ttggctccgg ccaagggagc aaagcacagc aagaagaggg tcggcagggg
     9301 cacgggctcc gggctgggca agacggcagg ccgcggatcc aagggccaga aatcccgttc
     9361 gggcggggga actcctccct ggtttgaggg cgggcagatg cctttgcagc ggcgagttcc
     9421 caagcgggga ttcaccaaca ttttcgccgc ccgcttcagt atcctcaacg tcaaggacct
     9481 ccaacgcttc gaacccgcgg gcaccgtgga tatccccgac ctcattcgtg ccggatacct
     9541 gaagaagggc agcgccggaa tcaaactctt gggagagggg gacatttcct tttccatcca
     9601 tgtccgggtc cacaaggcaa gtccctcggc catcagcaag gtcgaggcgg cgggaggccg
     9661 ggtggagctg atttccttac caaagaggac ctaacgggtg ctgacaggtt ttcagaatat
     9721 cgggaagatt ccggagctca agcgacggat catcaacacg ttgctgttgc tggccgtcta
     9781 ccgcatcggg gcgcacattc cgaccccggg catcaacacc gaagcactgg ccgcgttttt
     9841 ccgggcggcc cagggaacgc tcctgggcct cttcgacatg ttctccgggg gggctctcag
     9901 ccagctctcc gtgttcgccc tcgggatcat gccctacatc agctcctcca ttatcttgca
     9961 gcttctcacc gtggtcatcc cgcacctgga acggttgagc aaggaagggg aggcggggcg
    10021 ccgcaagatc acccaataca cgcgctatgg gaccgtgatt ctcagcatca tccagggctt
    10081 cggcatcgcg ttcggggttg agggaatgac gggacccggg ggggaaatgg tggtgctgta
    10141 tccgggctgg ggattccgca tcatcacggt catcaccctc acggccggga ccgccttcat
    10201 catgtggctg ggcgagcaga tcaccgagcg gggcatcggc aacggaatct ccctgatcat
    10261 ttttgccggg attgtggccg ggattcccaa tgccaccatc agttcgttcc ggctcctgag
    10321 cgcaggggaa atctcgcttt tcgtcgccct gcttctggtc atcatgatgg tcgtggtgat
    10381 cgggttgatc atcttcatgg agcgcggaca gcgcagaatc cccgttcaat acgccaagcg
    10441 cgtcgtgggt cgtcgggtct acggcggtca aaacacccac atccccttga aaatcaacac
    10501 ctccggcgtg atcccgccca tcttcgcctc ttccatcatc atgttccccg ccacggtggc
    10561 caacttcatc aaggtgccgt gggttcagtc ggtggccgat gccttttccc cgggccattg
    10621 gctttactcg gtcctgtacg tggggttcat cgtcttcttc tgcttcttct atacggccat
    10681 cgtcttcaat cccatcgatg tggccgacaa catgaagaaa tacggaggat tcatccccgg
    10741 catccgcccg ggcagaccca ccgccgaata catcgacaag gtgttgagcc gcatcacttt
    10801 gggtggtgcg atttacgtct ctgccgtgtg cgttcttcct accctgttga tccggaaatt
    10861 caacgtgcct ttctattttg gtgggaccgc gctcctcatc gtcataggag ttgccatgga
    10921 cacccttgct cagattgaag cacacatgct ctcacgtcac tatgaaggtt ttctcaagaa
    10981 ggggcgaata cgcgggagac ggtaggactt gcagaccctg ggtagggggg tgctccggca
    11041 gggtcgaggt catgagacag ggtgttttga cgcaatgatt cagaaaggga ggaacgacca
    11101 tgaacatcat cttgctgggt ccaccgggtg cggggaaggg aacacaggcc aaacgcatga
    11161 tcgatcgcta ccatatcccg cagatctcta cgggggacat gctccgggcc gccctgaagg
    11221 aagggaccgc tttgggactg gaagcaaaaa aatatatgga ccagggggcg ctggttcccg
    11281 atacggtggt ggtgggtctg gtgagggagc gaatccaaaa ggacgattgt tctcagggtt
    11341 tcatgctgga cggatttccc aggaatgtga gccaggcgga atccctggac ggaatgctcc
    11401 tggagttggg aaagaagatc gataaggtcg tgtgcatcga ggttcccagc tcagagcttc
    11461 tggcacggct caccgggcgc cgaacctgcc gggcctgcgg ggcgggcttc cacgtgatgt
    11521 ttgatcctcc caaaaaggac aaggtgtgcg acaagtgttc cggggagctg tatcagcggg
    11581 acgacgacaa cgaagccacc gtgtcttcca ggctccaggt ttatgaagcg caaaccaaac
    11641 ccctgatcga ctactacgag aagcaggcaa agctgctccg aatcaacggg ttgggaagca
    11701 tccaggaaat tttcggccgt gtggtttcag cccttggata gggggcccgg gattgatcat
    11761 tctaaggtcc gccagagaga tcgagaagat tcgcaaggcc tgtttggttg tggcggaaat
    11821 ccttgagcga ctccgagagc acattcgccc caacatcaca acctgggatc tgaatgtgct
    11881 cagcgaggag cttgcagcca gaaagaaggc caaaccggcg ttcaagggat accacggcta
    11941 tccgcactcc ct
//
LOCUS       DAOOVC010000118        11879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3525_length_11879_cov_6.510825, whole genome shotgun sequence.
ACCESSION   DAOOVC010000118 DAOOVC010000000
VERSION     DAOOVC010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3525_length_11879_cov_6.510825"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1704
                     /gene="serA"
                     /locus_tag="PLM79_13425"
     CDS             124..1704
                     /gene="serA"
                     /locus_tag="PLM79_13425"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOV87359.1"
                     /translation="MKLLACDGLHETGLALLQSAEGIQVDLGDSLSAAEIEARIGNYD
                     AVIVRSRTKVTAKLLERASRLRVIGRAGTGVDNIDVAAASSKGILVMNTPGANATAAA
                     EHTMAMMLALARHIPQANQSMREGRWEKKQFMGTELFHQTLGIIGLGRIGSIVADRAL
                     GMKMDVLGYDPYILPEAAAILGVEWVSLEELLARADFITLHTPSTEETRKILNRKTLA
                     QTKAGVRIINCARGDLVDEEALLESLVSGHVAGAALDVFNKEPPTDSPILKLPNVVST
                     PHLGASSLQAQANVARNIAAQILDYLFQGIIRNAVNFPSIRPKDYERIKPYLVLADRL
                     GRLQGQMCTPPQRLEIEFGGPDFQEIPTQPLTQSVIKGLLDPILSEKVNLVNAPLLLK
                     ERQIELVTSTTSEGRGYTGTITVRVRGREAECSAAATVFQEGEIRLVRLNNYRLEAEL
                     DGINLVIQNLDKPGTIGFIGTTLGHYSVNIADMHLSRTPERDRAVAIIRLDHPAPPEA
                     MEALRKHPNILSVQQVTI"
     gene            complement(2092..3183)
                     /locus_tag="PLM79_13430"
     CDS             complement(2092..3183)
                     /locus_tag="PLM79_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87360.1"
                     /translation="MKLTRRKARFLNAGIDEWTAEGILTAEEGRKLRDTIEVVNFDWM
                     RLAKYSFWISLLCLVIAFGAVLGDRLLMGFLAEIFTEERFAWYLCLFFALLACGFYWL
                     GIRRNRKFPERVFSNEALFFLGVMATAAFVVFLGRALDTGSGQFSTLLLISFLIYAVL
                     GLWFPSKLVWVFALLSLGGWFGARTGYVSGWGAYYLGMNYPLRFVFFGLGLTVSSVLF
                     DHIPARREFLKPTRAVGLLYLFIALWILSIFGNYGDIQDWFKAEQWMLLHWAAFFALF
                     AILAILYGVQFDDPMTRGFGITFLFINLYTRYFELFWDSLHKALFFALLALSFWVVGS
                     HAEKIWNVSILRRRPPPKQGVPNPGEPPS"
     gene            complement(3256..6690)
                     /locus_tag="PLM79_13435"
     CDS             complement(3256..6690)
                     /locus_tag="PLM79_13435"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87361.1"
                     /translation="MFPMEFDSQVLSTLPLPGGERIQSVLQVLKVIVLRRVLPYFKRT
                     RGTDSLRLGEISEHLSMVRFEDEENRVRLISLLTGKGPHRSVLIHERVFDYLSFVIPS
                     DPESTLGGASAEERKVLAFAELLLRHQVEHLLYPDEKEREVILGDVGFVMERREKDPT
                     FYRTLRSALSDEMNGIQGRAYLALFDAAEQGLTLDGPVNSILNDCIPGLGDLPPRLIQ
                     ELFPGWDVEIKGRILGECYRRSMSASHTLMERSGFLRTVFRLFAVAVAENEAQALEVL
                     HGFKDKWGLVGLFHELKLAESSVDEKSPRELLALLASALPARPDGEAPVSLPVAVLPH
                     SREPKGAVPRTKTLQDRIEEARNNPNVPRRVLEVIDRNKLNAVGHSGSKYTEFIETLL
                     AIPWGKIHPIRVTPREFEEGLNRSHYGLQTPKEILSDFFTNLIWCYRDFNEAEASVGQ
                     RTGSSFLLVGPPGVGKTSLAISVAQNLGIPYHKLSLGGMRDEADIRGHGFTYEGSKPG
                     AIVQGLIKMEAMNGMFILDEADKTEKFALSTLLEILDPEQNHLFHDKYTQTTVDIDLS
                     NCHFFLTANTLDGVPAVIVNRCEVVQLDRYSVEEKIAIARRYLVRRVRERYRIPREQV
                     FFEPAEEEDLLRFLIRTYTHEAGVRELERILRTLFLRLQRKEVLGGRELFVAVTRERI
                     KQVLKEPTRPRQIGEEDAVGEMLGLGVDMERGVGSVIPIQATRIGIGAGGGGTRRGAL
                     SIVHATGNIEKVMDESRKVATTGILSCAEALGIRIPGENEPVHLHFMGGSTRKDGPSA
                     GGAIALALASLLSGRAVRRDVAMTGEIDTKGRITSVGALDIKIETACNAGCKTVIFPR
                     ENLYGEGGIERFPDALKEELQILSFEEWAGPHPPFDYRRHILQVVAVENIVQAFKVAV
                     LDEGELKALEERFTEHARHVAEVLRSASPTPVVHLQAFQVKTPAELDPEWLRTLVGES
                     DIAPALLVLPGILETVREQTAEARGLLRIFAFEPKTQKLSDLLEELLRSVQSSSSQPV
                     RASLVAPFFLLLRDGILVKDFVNRPGMEGLRLFANNYTRQGVKLKGCKPVLNRVYSVL
                     SMLDDRQLNECPFLANDDGVYVVDLDFIPEKVRLDERRAEKILCACMTRWLSAARP"
     gene            complement(6946..8022)
                     /locus_tag="PLM79_13440"
     CDS             complement(6946..8022)
                     /locus_tag="PLM79_13440"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOV87362.1"
                     /translation="MEIKVQPIDPARRNAKPADESKLVFGKTFSDHMFMMDYESGVGW
                     RDARIVPYQDLCLDPAAMVLHYGQGIFEGMKGYRCRDGKIRLFRPQKNFERFNRSAFR
                     VCMPEIPTETQFEILEALLKLDKDWIPHSVGSSLYIRPTMIATEAALGVRPAKEYLYY
                     IITGPVAAYYAEGFNPVKIYVTDEFIRTVRGGVGEAKTMANYAASLFAAEMAKRKGFT
                     QVLWLDGVERRFVEEVGTMNIFFRIRDELITPPLSGSILPGVTRDSVIQLARHWGIQV
                     TERLIDIDEVMDAVQSGDMKEIFGTGTAAVISPVGEVFYRDQRLRVGNGGVGQWSQKL
                     YDEIVGIQYGEREDPFGWTYQMNI"
     gene            complement(8104..10767)
                     /locus_tag="PLM79_13445"
     CDS             complement(8104..10767)
                     /locus_tag="PLM79_13445"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOV87363.1"
                     /translation="MFQWLQRTKGEEPLSIRKDPFGYSVDQKPSFLFRGWFEKLISKT
                     FMPEDQQKLLDDLGKKGTLVYAVKYRSQLDFVFLSLRLHQLGLPAPQFLFDLHPYAWQ
                     PRWYALKLCGAHLFHYLRNGSLPNPYEGKHYREKVMGGSSGLFFLVGKQGYYERTVRV
                     GNDPLEHLLDIQKQMARPVFIVPLVLLYTRDPGRERRGLLEIFLGQREEQGLLRKVVS
                     FFRSYPNAVLEAGEPLNLQEVLPELSEALGERRKQIFQLRRNLIDSTDTLKRAIVGPL
                     LKSKIELKDIVLHHPRLETFMQRRARSSKQEIWKVRMEADQYLEEIAAGFNFTLFALG
                     EKVLGWMWNNLFDGVHVDMESLHRVKKAARNNTLVYIPTHKSHMDYLILSHVLFHNNL
                     SLPVVAAGKNLAFWPLGPIFRRAGAFFIRRSFKGLKFYAEVFSLYVKTMVQLGHNIEF
                     FIEGGRSRTGKMVLPKLGLLAILIQAVEEGFCPDLVFVPVSICYDHIPEEEAYLKEIT
                     GGVKVEENLGQLVRARRFLKKRYGRVYVQFAEPMSLQRYLARYRGAQDELRPRERHAM
                     YRDFAYRIINSINQVSLVTPHALAASAFLTTASHGVSGAEFQEVCRTFYDYLREQQVR
                     FSNTFRSYNVTIEETLHDLERSKLIGKLKDEDDDLEEEVFTMEDHKRLALEYYKNNVI
                     HFLLPAACVSTSILAQQTFRFSVAQVLEDVAFMKNFFKYEFVYDNEVGNEDLVREVLD
                     IFERMGLLHRMESNDRPFLLSHKGLKAAYAFHGLLRNYFEGYWIVLRSFRYLQKKPYS
                     EKDFLKKVLGLGQKALKLELIERPESISKILFSNALQYYLENGVIEKRVDVKGKDRDQ
                     EWYADAGNRPLVQEYSRQISRFLRSPYFTLQ"
     gene            11085..>11879
                     /locus_tag="PLM79_13450"
     CDS             11085..>11879
                     /locus_tag="PLM79_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87364.1"
                     /translation="MLTELLVHNLAVIRELELSLDAGLNIVSGETGAGKSILVGAVSL
                     LLGGRASQEMVRTGAAEATVQGIFSLSNPLPWNRKLESLGIPPSDRLTVRRSIHRNGR
                     NRVFVNDRMVSVQQLQQLARGLASISGQHEHQILLDPEIHLSLLDQFGGLDAQVAKVG
                     ALFSQWSQHREALHRLRRSKEERADRADFVRFQLQELLSAKLHPEEDRTLEHELNLLK
                     HAAALNDAAKSAYEILYSGRGAVVEQLAFLEKNLDTLVRIDPGRASF"
BASE COUNT         2462 a         3545 c         3563 g         2309 t
ORIGIN      
        1 atccgaaggg cttacttggg ttaaaccgtc gagggcgttc caggcccgtt cttcggaacc
       61 ttcgggactc ccgagaggag aggaactcct gggtccaacg gctacggaag agaggaacaa
      121 cccatgaaac tgctcgcttg cgatggattg cacgagacgg gcctggccct gctccaatcc
      181 gccgagggga tccaagtgga cctgggagat tccctgtccg ccgcggaaat cgaggcaagg
      241 atcgggaatt atgatgccgt catcgttcgc agccgcacca aggtcaccgc caagctcctg
      301 gagagggcga gccgcctccg ggtgatcggc agggcgggaa ccggggtgga caacatcgac
      361 gttgcggcgg caagcagcaa gggcatcctg gtgatgaaca ctccgggggc caacgccacg
      421 gcggcggccg agcacaccat ggccatgatg ctcgccctgg cccgtcacat tccccaggcc
      481 aatcaatcca tgagggaggg ccgctgggag aagaagcagt tcatgggaac ggaactgttt
      541 caccagaccc tggggatcat cgggctgggg aggatcggct ccattgttgc cgaccgggcc
      601 ttgggcatga agatggacgt cctgggctac gatccctaca tccttcccga ggccgcggcc
      661 atcctggggg tcgagtgggt gagccttgag gaactgctgg cccgggcgga tttcatcacc
      721 cttcacaccc cttccaccga ggaaacgcgc aagatcctca atcggaagac cctggcgcaa
      781 accaaggcgg gggttcggat catcaactgc gcccgggggg acctcgtgga cgaggaagcc
      841 ctcctggaat ccctcgtttc gggacacgtg gcgggggcgg ccctggacgt gttcaacaag
      901 gagcccccca cggacagccc gatcctgaag ctccccaacg tcgtctcgac cccgcacctg
      961 ggggcatcga gtctccaggc ccaggccaac gtcgcccgca acatcgccgc ccagatcctg
     1021 gactacctgt tccagggaat cattcgcaac gccgtgaact tcccctccat tcgtcccaag
     1081 gactacgaaa ggatcaagcc ctacctggtg ctggcggacc ggctgggaag gctgcagggg
     1141 cagatgtgca cgccgcccca gcgcctcgag atcgagttcg gaggcccgga tttccaggaa
     1201 atccccaccc agcccctgac ccagtccgtc atcaaggggc ttctggatcc catcctgtcc
     1261 gagaaggtga acctggtcaa tgcgcctctg ctcttgaagg aacgccagat cgagctggtc
     1321 acctcgacca cttccgaagg ccgcggatac accggaacca tcaccgtccg ggtcaggggc
     1381 agggaggcgg aatgctccgc cgcggccacg gttttccagg aaggagagat ccgcctggtg
     1441 cgcctgaaca attatcggct ggaggccgag ctggacggca tcaacctggt catccagaac
     1501 ctggacaaac ccggcaccat cggcttcatc ggcaccaccc tgggacacta ctcggtcaac
     1561 attgccgaca tgcacttgag ccgaactccc gagagggacc gggccgtcgc catcatccgc
     1621 ctggaccacc cggctcctcc cgaagccatg gaagccctgc gcaagcaccc gaacatcttg
     1681 tccgttcagc aggtcacgat ctaggctgcg tatgggaccg ggtgccgaag cttcccggtc
     1741 gttttggggt gcacccctca tgaatgcccg gcggggcagg aggaaagccg ttgcattgag
     1801 gattccgtct ccctccgggg gagaatgagg aaagatcggc cttatacgaa attgcactca
     1861 agtcgaaact gcccacccgc aggattcagc cctttgctcg taatctgcac tcagcatgtc
     1921 agggcgacct tgagcgcaac ttggtattac aaaccccaac cctctactcc gaagcgggag
     1981 agagggaggc gatgcgggga ccgcaattgt acgacattgg cgggcggcgg tgggtggtcg
     2041 gaacaggggc aagcaaggcc tttgtcacgg atctgcggga agtcgaaccc gttaggaggg
     2101 aggttcgccc gggtttggaa ctccctgctt cgggggaggg cggcgtcgca ggatggacac
     2161 gttccagatc ttttccgcgt gggaaccgac cacccagaag ctgagggcca ggagcgcaaa
     2221 gaagagggcc ttgtggagcg aatcccagaa cagctcgaaa tagcgtgtgt acaggttgat
     2281 gaacagaaaa gtgatcccga acccacgggt catggggtcg tcgaactgga ctccgtaaag
     2341 aatcgccagg atcgcgaaga gggcaaaaaa agccgcccag tgcaaaagca tccattgctc
     2401 ggctttgaac cagtcctgga tgtcgccgta gtttccaaag atcgacagga tccacagggc
     2461 aatgaaaaga tagagcagtc ccacggcccg ggtgggcttg aggaactccc gtcgggccgg
     2521 gatatgatcg aagagcacgc tcgagacggt caatcccagc ccgaagaata cgaagcgcag
     2581 gggatagttc atccccaggt aatacgcccc ccatccggac acataccccg tcctggctcc
     2641 aaaccaccct cccagggaaa ggagtgcgaa cacccagacc agcttcgagg ggaaccagag
     2701 tcccaggacc gcgtagatga gaaaggagat caggagcagg gtcgagaatt ggccgctgcc
     2761 cgtatccagg gccctaccca ggaaaaccac gaacgccgcc gttgccatga ccccgaggaa
     2821 gaagagcgct tcattgctga agactctctc ggggaatttc cggttcctcc gtattcccag
     2881 ccagtagaag ccgcaggcga gaagggcgaa aaacaggcac aggtaccagg caaagcgctc
     2941 ttccgtaaag atctccgcca ggaatcccat caacaggcga tcgccgagaa cggcgccgaa
     3001 tgcaatgacg aggcagagga gggaaatcca gaaggaatac ttggcaaggc gcatccagtc
     3061 gaagttcacc acctcgatgg tatctcggag ctttcggcct tcctcggccg tgagaattcc
     3121 ttcggccgtc cactcgtcga tgccggcgtt caaaaaccgg gcttttctcc ttgtcagctt
     3181 caagaacgtc tcctccttgc ggaccgggcc ggggaaccat ggaccgggtc cccgccgtca
     3241 aagggagcct cggggctatg gcctcgccgc cgagagccac cgggtcatgc aggcgcaaag
     3301 gatcttttcg gcccgtcgct catccaaccg aaccttctcc gggatgaagt cgagatccac
     3361 cacgtagacc ccgtcgtcgt ttgccaggaa gggacattcg ttgagctgcc ggtcatcgag
     3421 catggacagc accgaataca cccggttgag gaccggcttg cagcctttga gcttgactcc
     3481 ctgccgggtg tagttgttgg caaagaggcg aagcccttcc attcccggcc tgtttacaaa
     3541 gtctttcacc aggatgccgt ctcggaggag aagaaagaaa ggggcgacca gggacgcccg
     3601 gacgggttgg gaagaagagg attggacgga tcggagcagc tcctccagga ggtccgacag
     3661 cttttgggtt ttgggctcga aggcaaagat ccgcagaagg ccccgggcct cggccgtctg
     3721 ctcgcggacg gtctcgagga tcccgggaag caccagcaat gccggggcga tgtcggattc
     3781 cccgacaaga gtgcgaagcc actccgggtc caattccgcg ggggtcttta cctggaaggc
     3841 ctggagatgg accacgggcg tcggggaggc gctccgcagg acttcggcta cgtgccgggc
     3901 gtgctccgta aatcgctctt ccagggcttt cagttccccc tcgtccagca ccgccacctt
     3961 gaatgcctgc acgatattct cgacggccac cacctggaga atgtggcgcc ggtaatcgaa
     4021 gggcggatgt ggcccggccc attcctcgaa ggagagaatt tgcagctctt ccttgagggc
     4081 atccgggaag cgttcgatgc ccccttctcc gtacaggttt tcccggggga agatcaccgt
     4141 tttgcagcct gcgttgcagg cggtttcgat cttaatgtcc agggcaccca ccgaagtgat
     4201 cctgcccttg gtgtcgatct ctccggtcat ggccacatcc cttcgcacgg cccggccgga
     4261 aagaagggaa gccagggcca aggcgatggc acccccggcc gacgggccgt ctttgcgggt
     4321 cgaacctccc atgaaatgaa gatgcaccgg ctcgttctcg ccgggaatcc ggattcccag
     4381 ggcttcggcg caggaaagga tgccggtggt ggccaccttc cggctctcat ccatgacctt
     4441 ttcaatgttt cccgtggcat gaacgatgct cagagctccg cggcgggtgc ccccgccacc
     4501 ggccccgatt ccgattctcg ttgcctgaat ggggatgacg gaacccaccc ccctctccat
     4561 gtccactccc aggcccagca tctcccccac agcgtcttcc tccccgatct gccggggccg
     4621 ggtcggctcc ttcagcacct gtttgatccg ctcccgggtg accgcaacga agagctcccg
     4681 tccccccagg acctcctttc gctgcagccg aagaaagagg gtccgcagga ttcgctcgag
     4741 ctcccgcact ccggcctcgt gggtgtaggt gcggatgagg aaccgcagga ggtcttcttc
     4801 ctccgccggt tcgaaaaaga cctgctcccg gggaatccgg tatcgctccc gaacccgacg
     4861 aaccaggtaa cggcgggcaa tggcgatctt ttcctcgacg ctgtaccggt ccaactggac
     4921 cacctcgcac cggttcacga tgacggcggg cactccgtcc agggtattgg ccgtaagaaa
     4981 gaaatggcag ttggacagat cgatgtccac ggtggtctgg gtgtacttgt cgtgaaagag
     5041 atgattttgc tcggggtcca ggatctcgag gagggtggac aaggcgaact tctcggtctt
     5101 gtcggcctcg tccaggatga acatgccgtt catggcttcc atcttgatga ggccctggac
     5161 gatggctccg ggcttggatc cctcgtaggt gaacccgtgt cctcgaatgt ccgcctcgtc
     5221 cctcatgccg cccagggaga gcttatggta gggaattccc aggttctggg ccacggaaat
     5281 ggccagggag gttttcccca ccccgggagg ccccaccagg agaaaagagc tgccggtccg
     5341 ctgcccgacg gacgcttcgg cctcgttgaa gtcccggtag caccagatga ggttggtgaa
     5401 aaaatccgag aggatctcct tgggcgtctg cagcccgtaa tgggaacgat tgaggccctc
     5461 ttcgaattcc cggggagtca cccggatggg gtgaatcttt ccccagggaa tggccagaag
     5521 ggtctcgatg aattccgtgt acttcgaccc gctgtggccc acggcgttga gcttgtttcg
     5581 gtcgatgact tccaagacac gccggggaac gtttggattg ttccgggctt cctcgatacg
     5641 gtcctggagc gttttggtcc ggggcacggc ccctttgggc tccctcgaat gcgggaggac
     5701 ggcaacgggg agcgaaacgg gggcttcccc gtccgggcga gccggaaggg ctgaggccaa
     5761 cagggccagg agctcccggg gagacttctc atccaccgag gattccgcca gctttagttc
     5821 atggaaaagc cccaccaggc cccacttgtc tttgaacccg tgaagaacct ccaacgcctg
     5881 ggcctcgttt tccgcgacgg ccacggcgaa gagccgaaag acggtgcgca gaaagccgga
     5941 tcgttccatg agcgtgtggg aggcgctcat actcctgcga tagcactccc cgaggatcct
     6001 ccccttgatc tcgacgtccc atccggggaa cagctcttgg atgagccggg gcggaagatc
     6061 cccgagtccc gggatgcagt cgttcaggat ggagttcacg ggtccgtcca aggtgagtcc
     6121 ttgttccgcc gcgtcgaaga gggccagata ggcccgaccc tggattccgt tcatttcatc
     6181 ggaaagggcg cttcgaaggg ttcggtagaa ggtggggtcc ttttcccggc gctccatgac
     6241 aaaacccacg tctccgagaa tgacttcccg ctccttctcg tccgggtaga gcaggtgctc
     6301 cacctggtgt cgcaaaagca gctcggcaaa ggccaggacc tttcgctctt cggcggaagc
     6361 tccccccaag gttgactcgg gatcgctggg gatgacgaag gacaggtaat cgaaaacgcg
     6421 ctcgtggatg agcacggagc ggtgggggcc tttccccgtg aggaggctga tgagtcggac
     6481 ccggttctct tcgtcctcga accgaaccat ggacagatgc tcggagatct cgcccaggcg
     6541 aagggaatcc gtcccccgtg tccgcttgaa atagggcaat acccggcgca gcacgatgac
     6601 cttgaggacc tggagaacgc tctggattcg ctccccgccc ggcaacggca gggtgctcaa
     6661 cacctgggaa tcaaactcca tgggaaacat gtgcgccctc ccgaagctgg agtcggatgg
     6721 tttgtccggg cacgggaaag gcccggcaga gcccgggact cgaaggggtc ctgggtagcg
     6781 gttccacccg tgcaagaacc cgtggaaaaa agggaaagcc ctggtttgcc ggagggggta
     6841 agcaaaccag ggcttggagg ggttaggaga aggcgacaca ctgtcaacct tgccctaaaa
     6901 gtaaccgacc attgaataaa tttcaattgc tagtgaaatt tatccctaaa tgttcatctg
     6961 gtaggtccag ccgaagggat cttcccgctc tccgtattga atccccacga tctcatcgta
     7021 gagcttctgg gaccattggc ccactcctcc atttcccaca cgcaggcgct gatcccggta
     7081 gaagacctct cccacggggg aaatcaccgc cgccgttccc gttccgaaga tctctttcat
     7141 gtcccccgac tgaacggcgt ccatgacctc gtcaatgtcg atcaaccgtt cggtcacctg
     7201 gatgccccag tggcgggcca actggatcac cgaatcccgg gtcaccccgg gaagaatgct
     7261 gccggagagg ggcggtgtga tgagttcgtc ccggatccgg aagaagatgt tcatggtgcc
     7321 gacttcttcc acgaacctcc gttccacccc atccagccaa aggacctggg tgaacccctt
     7381 ccgcttggcc atttcggcgg caaagaggct ggcggcgtaa ttggccatgg tcttggcttc
     7441 tcccactccc cccctcaccg tacggatgaa ttcgtcggtg acgtaaatct tcacggggtt
     7501 gaatccctcg gcgtaataag ccgccacggg acccgtgatg atgtagtaga ggtattcctt
     7561 ggccgggcgg actcccaggg cggcctcggt ggcgatcatg gtcggccgga tgtacaggga
     7621 ggatcccacg ctgtggggaa tccagtcctt atccagtttc agcagggctt ccaggatctc
     7681 aaactgtgtt tccgtgggga tctccggcat gcagaccctg aaagcggacc gattgaatcg
     7741 ctcgaaattc ttctgcggac gaaagaggcg aatcttgccg tcccggcatc gatacccttt
     7801 cattccctcg aagattccct ggccgtagtg gagcaccatg gccgcagggt ccaagcacag
     7861 gtcttgatat gggacaatcc tggcatcgcg ccaccctacc cccgattcat agtccatcat
     7921 gaacatgtgg tcgctgaagg tctttccgaa gacgagcttg ctttcgtccg ccggtttcgc
     7981 attgcgtctt gcagggtcta tgggttgcac tttgatttcc atcgtaaagc ctccgtccgc
     8041 gaattcgcac tctgaagagt atttctcata tcgagcatgg agtctatagc acgattcatc
     8101 ctcttattgg agggtgaaat aaggagagcg gagaaagcgg ctgatctgtc ggctgtattc
     8161 ctggaccagg gggcggttcc ccgcatcggc ataccattcc tggtcccggt ccttcccctt
     8221 gacgtccacc ctcttttcga tcaccccgtt ttccaggtag tactgaaggg cgttggagaa
     8281 gaggatcttg gagatggatt cgggcctttc gatgagctcc aatttcaggg ctttttgtcc
     8341 caggccgagg accttcttga ggaaatcctt ttcgctgtac ggcttcttct ggagataacg
     8401 gaaggaccgg agcacaatcc agtagccttc gaagtagttt cgaagaagac catggaaagc
     8461 gtaggccgcc ttgagtcctt tgtggctcag gaggaagggt cgatcattgg attccatgcg
     8521 atggagcagt cccatcctct cgaagatgtc caacacctct cggaccaggt cttcgttgcc
     8581 gacttcattg tcgtagacaa actcgtactt gaagaagttc ttcatgaagg ccacgtcttc
     8641 cagaacctgg gccacgctga aacgaaaggt ctgttgggcc aggatggaag tggaaacgca
     8701 ggccgccgga agcaggaagt ggatcacatt gttcttatag tattccaagg ccaggcgttt
     8761 gtggtcctcc atggtgaaga cttcctcctc gaggtcgtcg tcctcgtcct tgagcttgcc
     8821 gatcagcttg cttcgttcga ggtcgtggag ggtttcctcg atggtgacgt tataactccg
     8881 gaaggtattg gaaaaccgga cctgctgttc ccgcaggtag tcgtaaaagg tccggcagac
     8941 ttcctggaac tcggcgccgg acaccccgtg gctggcggtc gtcaggaagg ccgaggcggc
     9001 caaggcatgg ggggttacca gggacacctg gttgatgctg ttgatgatcc gataggcaaa
     9061 atcccggtac atggcgtgcc gttcccgggg tctcaactcg tcctgggctc cccggtagcg
     9121 ggccaggtag cgctgaaggg acatgggttc ggcgaattga acatagacgc gcccatagcg
     9181 tttcttgaga aaacgcctgg cccgaaccag ttgacccaga ttttcctcga ccttgacccc
     9241 gccggtgatc tccttcaagt aagcttcctc ctcgggaata tggtcgtagc agatggatac
     9301 gggcacaaac accaggtccg ggcagaagcc ttcctccacg gcctggatca agatggcaag
     9361 aagcccaagc ttggggagca ccattttccc cgtccggctt ctgccccctt ctatgaaaaa
     9421 ctcgatgttg tgccccagct gaaccatggt cttcacatag agggaaaaga cctcggcata
     9481 gaatttgagc cccttgaagc tgcgacggat aaaaaaggcc ccggcccgtc gaaatatggg
     9541 acccaggggc cagaacgcca ggttcttccc ggccgccacc acggggaggg aaaggttgtt
     9601 gtgaaagagc acgtgggaga ggatcaggta atccatgtgg ctcttgtggg tggggatgta
     9661 gaccagcgtg ttgttgcggg ccgccttttt cacgcggtga aggctttcca tgtccacgtg
     9721 gactccgtcg aagagattgt tccacatcca tcccaggacc ttttccccca gtgcaaacag
     9781 ggtgaagttg aaaccggcgg cgatttcctc gagatactgg tcggcttcca tgcggacctt
     9841 ccagatttcc tgcttgctgg accgggcgcg ccgctgcatg aaggtctcca gccggggatg
     9901 gtgcagaacg atatccttga gctcgatctt gctcttgagc aggggcccca cgatggcccg
     9961 cttgagggta tcggtggaat cgatcaggtt ccttcgcagc tgaaagatct gtttgcgccg
    10021 ttctcccagg gcttcggaaa gctccggcag cacctcctgc aggttcaaag gctcgcccgc
    10081 ctccaacacc gcgttggggt agctgcgaaa gaaagagacc acctttcgaa gcagtccctg
    10141 ttcttctctc tgtcccagga aaatctccag gagaccccgt cgttctcgtc ccgggtcccg
    10201 ggtgtacagc agcacaaggg gaacaatgaa gaccggccgt gccatctgtt tctggatgtc
    10261 taggaggtgc tcgagggggt cgtttcccac ccgaacggtt cgttcataat acccctgctt
    10321 gcccaccagg aagaacagcc cggaagagcc gcccatgacc ttttcccggt agtgcttccc
    10381 ctcgtaaggg ttgggcaggc tgccgttccg caggtagtgg aagaggtgag ccccgcaaag
    10441 ctttaacgcg taccagcggg gctgccaggc gtaaggatgc aggtcgaaga ggaactgagg
    10501 ggcgggaagg cccagttgat gaaggcgaag ggagaggaac acgaaatcca gttgagatcg
    10561 gtatttgacg gcatagacca gggtgccctt cttgcccaga tcgtccagca gcttctgctg
    10621 gtcttcgggc atgaaggttt tggaaatcag cttctcgaac cagcctcgga acaaaaagga
    10681 cggcttttgg tccacagaat acccgaaggg gtcttttcgg atggaaagag gttcttcccc
    10741 ttttgttctc tgcaaccatt gaaacatggg tgggcacctt cttctgttga gtccgttccc
    10801 gggagactcc tcggcccgac tcccaaaccc gcccaaaaca aggaccaacc gggttcaagg
    10861 gggtcccgtg ccgccgattc ccctccagcg gtcggccatg aaatcgaggg catgtataaa
    10921 gcaagatacg ctatcaaaaa aggggggaga aaccaaacta tttgtgggaa attgacggat
    10981 gcgaccaccc gcccccccgg gcttcccgcc tcaaaaatgg ttgattgtgg aatgggactc
    11041 ctccatagtg atgggcacct tcaacggcaa cgggagaacc accggtgttg acagagctgc
    11101 tcgtacacaa ccttgccgtc atccgggagc tcgagctttc cctcgatgcc gggctcaaca
    11161 ttgtttcggg agaaaccggg gcggggaaat ccatcctggt gggagcggtg agcctgcttc
    11221 tcgggggacg cgcatcccag gagatggtcc gtaccggggc cgccgaggcc accgttcaag
    11281 gcatcttttc cctctcaaat cctttgccgt ggaaccggaa gctggagtcc cttggaatcc
    11341 cgccttcgga ccggttgacc gttcgacgca gcatccaccg caacggtcga aaccgggtct
    11401 tcgtgaacga ccggatggtg agtgtccaac aactccagca gctggcccgc gggttggcct
    11461 ccatttccgg ccagcacgag caccagatcc tcctggaccc cgaaattcac ctcagtcttc
    11521 tggaccaatt cggaggactc gatgcccaag tggctaaggt cggtgccctc ttctcccagt
    11581 ggtcccaaca ccgggaggct ctgcaccgtt tgcgtcgcag caaggaagaa cgggccgatc
    11641 gagccgactt cgtccggttt cagctccagg agctcctgtc ggccaagctc catcccgaag
    11701 aagaccggac cctggaacac gaattgaatc tgctcaagca cgccgccgct ctcaacgacg
    11761 cggcaaaatc cgcctacgag atcctctact ccgggcgagg agccgtagtg gagcaactgg
    11821 ctttcctcga gaagaatctc gacaccctgg tccgaatcga tccgggccgg gcatctttc
//
LOCUS       DAOOVC010000119        11713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3585_length_11713_cov_6.237862, whole genome shotgun sequence.
ACCESSION   DAOOVC010000119 DAOOVC010000000
VERSION     DAOOVC010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11713
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_11713_cov_6.237862"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="PLM79_13455"
     CDS             <1..1821
                     /locus_tag="PLM79_13455"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOV87365.1"
                     /translation="ILDEPNTQQVVWDKFRRAFNFQNISVDDLAFIYENTLIREETRK
                     RFGIHSTPPAIAELMVDRLPVESLPQDERYVLEPCAGHGVFLIAALRRLRELLPVSWT
                     NQEKHAYLKDRLTAIEMDTFAAEVCRLSLTLADYPNPNGWKILSEDIFATDVLYRQMK
                     TGRIILCNPPFEDFTPEERGRYGQRVQSVHKPYEVLRRVLTDPPAMLGFVLPKSAIIG
                     GRYGDLQDQIARLYKNIETVALPDRIFAFSDQETVLVLASERNDSEQADVQTRTFWIR
                     DNDRQPLIEKSRLPDEVSKVICRSAAKKPLRIWNPPLAELWEYLERYPRLQEVAEIHR
                     GIEWNISLRENRDRLISPIERPSFVHGLDTAQGKIEPFHAKGFVYLNMDPQYQRTTAH
                     KFAWDKPKVIVNSHRMSRTPWRIVGYPDSKGFVCYQNLFGIWPTNEMNIEVLAAIINS
                     PIINAVFFIQNYGRHNLVDTLEQIPVPLLSKVDGARVTDLVKQYSTSRRQFENPADRA
                     TAVQDSIRTLMKIDALILKAYDLPPKLERKLLEFFRGYKRPLPFEFPDYYPEDFKPYV
                     PLYKFIEMDLKQASAGELLKRITPLDSEEAHDLVLTLEQR"
     gene            1829..2341
                     /locus_tag="PLM79_13460"
     CDS             1829..2341
                     /locus_tag="PLM79_13460"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOV87366.1"
                     /translation="MDGYLLDTNAASVLWDARHADHDKIRAFLESVSPAPVWISIIAL
                     AEVEYGLKTAPAMNLDTHKDVRAEMAKYPEVLEPDKHTIAPYSDLRAELFKSYAPRDR
                     KGRLTKKWVEDLFERTSAKELHGSQENDIWVASLAIQYNLILVTEDRMAPIVEVSKYL
                     PDPLRIQKWR"
     gene            complement(2390..3103)
                     /locus_tag="PLM79_13465"
     CDS             complement(2390..3103)
                     /locus_tag="PLM79_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87367.1"
                     /translation="MDTQSPPSSAVLVVDTCSLLDIVRAPVFDRVSPTDMVNALWIVD
                     ALQTRKTCFHLVVCDWVQSEYRRAIGGVEQDVRQQLEKVWASHNHAAKIAAAFEAQDV
                     ELVDARKCADTVISRGKQIVGQFLALAGSIHASEEDERRAGLRTKEARPPAHRGKGSM
                     ADAVITELALRLSNEMRSGLREPGAVVLLSSNTKDFCEGKVLKTDLQHEFEASGIEYA
                     PTWSAARYACSSSSEGTPS"
     gene            complement(3118..3783)
                     /locus_tag="PLM79_13470"
     CDS             complement(3118..3783)
                     /locus_tag="PLM79_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87368.1"
                     /translation="MSDYGFLPVFRDLIITGGAAEIGRIGDVIEAQLHEGWVRDQARE
                     EESSLIEGSKSLDWLVFNRDSTPEIPAASLFMTKQDDDLAVTNIVPQTVSELSRQQYN
                     LILAEFFETAAEPACRALNLRCTLTEDTLPITHWISEEAASHLLRFSNLANKATGSAH
                     PLDRRRWFAFIAQTVRDESSLDAHTLGMWLVRVGHWPEDSAQQLMIEYEFGRDLLRYV
                     DAQ"
     gene            complement(3780..4940)
                     /locus_tag="PLM79_13475"
     CDS             complement(3780..4940)
                     /locus_tag="PLM79_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87369.1"
                     /translation="MGHWPKVRLSECLQLINDTVPVQRDQEYPNFGIYSYARGLFRKR
                     PIIGLESSATTLRRVRKGQFIYSRLFAFEGAYGIVGDEFDGFFVSNEYPTFICDEARL
                     MPHYLLAYFSMKRVWEAVAKGSKGLGVRRQRVQPEEILANEIPLAPIHVQRSIVSRLE
                     TVVAKARAVVDRLDAIEADAERLLAIRFRESMARADWRAMGEVAPVVRRDVEIEPEQS
                     YTELGVRSFYKGTFHRRTLPGSEFRWQKLFWVYRGDLIFSNIMAWEQAIAIAKPADHG
                     CVGNHRMLTCLPSPDITTSDYLWYYFTTQEGFASIAAASPGTAARNKTLTSDSLMAIR
                     VPVPLLSTQLSFNALQSKVAMMKAKHTSIRKDLDALLPTMLERVFHSERCSP"
     gene            complement(4944..6764)
                     /locus_tag="PLM79_13480"
     CDS             complement(4944..6764)
                     /locus_tag="PLM79_13480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOV87370.1"
                     /translation="MARRIPKAGGQPQTTAQRLNALIKSARDIMRKDKGLNGDLDRLP
                     MLTWIMFLKFLDDMEMIREQEAQIEGVKFKPVIESPYRWRDWAAKEDGITGDELIAFI
                     NQDEAIRPDGSRGPGLFAYLRNLQSANGRDRRDVIANVFRGVINRMINGYLLRDVINK
                     VNGIHFSSSEEIHTLGHLYEIMLREMRDAAGDSGEFYTPRPVVKFMVEVIDPKLGEMV
                     LDPACGTGGFLVEAYTHLAGQCRTVEERRILQERSIFGQEAKPLPYMLSQMNLLLHGL
                     EYPDLVYGNSLAVAIKEIGDKDRVDIIMTNPPFGGEEETGIKSRFPVEMQTSETALLF
                     LQLIMRKLKRSRQSQGKPGRAAVVVPNGTLFGDGVCARIKEDLLKNFNLHTIVRLPQG
                     VFAPYTDIPANLIFFDRRGPTREIWIYELPPPEGRKKYSKTAPIQYEEFAPCLDWWKK
                     REENGRAWRVDFAAIKADALSLAEPLWKKAESERQAAAQCTARLREIDTGLTALNGAE
                     GKSSNKSALQAEQQALIARRQAHEEAAKSAQREGDAVYWPIYNLDLKNPNGREAVEYI
                     PPEKLLSRMIENEREAAALLADIAALVKDDAGIMGSGERG"
     gene            complement(6788..9163)
                     /locus_tag="PLM79_13485"
     CDS             complement(6788..9163)
                     /locus_tag="PLM79_13485"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOV87371.1"
                     /translation="MNEADTCRKYVVPKLVAAGWDEEPHSITEQRSFTDGRIMVSGGK
                     VRRGKQKRADYLLRYTRDFPLAVVEAKSLYATSSSGLEQAKEYAEILGLKFAYSTNGK
                     GICEFDFITGNERELEAFPSPDELWERLRMSEGLNDEQAARLLTAFYLDFEKRARYYQ
                     EVAINRTVQAVLKGRKRILVTMATGTGKTFVAFQVCWKLWSSRWNSTGEYRRPKILYL
                     ADRNILIDDPKDKMFLPFGDARYKIENGEVVKSREMYFAIYQAIAKDERRPGLYREYS
                     PDFFDLIVVDECHRGSARDDSNWREILEYFKPAVQFGMTATPLREDNRDTYQYFGNPI
                     YTYSLRAGIEDGFLAPYRVHRVITTADALGWRPTKGELDRYGREIPDGEYQTGDFERI
                     VALRARTEAIAAHMAEFMRKTDRFAKTIVFCVDQEHAEEMRRALNNLNTDLVKDHPSY
                     ACRVTADEGSVGRGHLSRFQELESDTPVILTTSQLLTTGVDAPMVKNVALARVIGSMT
                     EFKQIIGRGTRVRDDYGKYFFSILDYTGSATRHFADPDFDGDPALVTEETVDDSGQVT
                     DEEIVTPEENSEEPGDEEIVSDPEPPVITDPQPNEPRKYYVDNGVIEIAAHMVYELNE
                     SGKQLRVVKYTDYTADVVRTLYPTAGELRSNWMRADGRDEIVEELSKRGIDFDELAST
                     LRLPEADPFDLLCHVAYSAPLRTRRERAERLRKERKDFFDQYGPEARAVLEDLLGKYA
                     EHGIGQFKIPDALKVPPVSDRGNVVEIIGYFGGADKLREAVATLQSELYAA"
     gene            10131..10400
                     /locus_tag="PLM79_13490"
     CDS             10131..10400
                     /locus_tag="PLM79_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV87372.1"
                     /translation="MEEYLTVKELCDRIKYKEQTIYNLISRGTFVLGRHFLKPTPKKI
                     LFKWSEIKAWIGDAALSGEVGAALDGPEIKDKTATQPGQVSLIRI"
     gene            10500..>11713
                     /locus_tag="PLM79_13495"
     CDS             10500..>11713
                     /locus_tag="PLM79_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HOV87373.1"
                     /translation="MAEVASHANVVTFPQRKKQSEKPRKAGLNRNREGSVRRINGKVY
                     VDFIYLGERVRETSGLEWNEKSAKQVREQLDKIIVSIKAGTFRFADVFPESKNCDFFT
                     AKEREVYRLKQTPEQVACKGFFDSWYELVKSSKRITGRTLLGYKRYMELYLSPFFGSM
                     TFADLNAQTFEKFIVWARQQHYRKKEISGNTINKCFTILKMICKTVSIEYGWGNTYNP
                     FFGFKKLPEDDPYEDIFPFSVEEQQQLIEHLPDHWKPYFRFAFCAGLRPGEQIAIKPE
                     DIDWKQGVLNIRRAMTLDEEGKKVVGRTKNRYSRRTIKLIPVMLDALQEQKRIHDRFQ
                     SEYFFCSTTGAQVNVHNLRARVWIPALKRAGLQFREMKQTRHTFATIALSCGESPLWI
                     ASVMGHRNTAMI"
BASE COUNT         2669 a         3277 c         3141 g         2626 t
ORIGIN      
        1 atcctggatg aaccgaatac acagcaggtg gtatgggaca agttcagaag ggctttcaac
       61 ttccagaaca tttcagtgga tgacctggca ttcatttatg aaaacacgct gattcgggaa
      121 gaaacccgga aacggttcgg tatccacagc actccgccgg ccattgccga gcttatggta
      181 gacaggctcc cggttgaatc attaccccag gatgagcgct acgtgttgga gccctgtgcg
      241 gggcatggcg tctttctcat tgccgcgctg cggcgtctga gagagcttct gcctgtgtcc
      301 tggaccaacc aggaaaagca cgcctacttg aaggatcggc tgacggccat cgagatggac
      361 acctttgccg ccgaagtctg ccgcctttcg ctcacgcttg cggattaccc caatccgaac
      421 ggatggaaga tcctttctga ggacatcttc gcaaccgacg tgctctaccg gcaaatgaag
      481 actggacgaa tcatcctctg caatccaccg ttcgaggatt ttactccgga ggagaggggg
      541 cgatacggcc agagggtgca gagcgtccac aagccctatg aagtgttacg gagagtcctc
      601 acggacccgc ccgccatgtt ggggttcgtg cttcccaaat ccgccatcat cggaggccgt
      661 tacggggatc ttcaggacca gatcgcccgt ctctacaaga acattgagac ggtagccctg
      721 ccggatcgta tcttcgcctt ctccgatcag gagacggtac tggtgcttgc ttcggagcgc
      781 aacgattcag aacaagccga cgtgcagacg cgaaccttct ggatacgcga caatgacagg
      841 cagccgttga tcgagaagag ccgactgccg gacgaagtga gcaaggttat ctgtcgttcg
      901 gctgcaaaga agcccttgcg catctggaat cctcccctcg ccgagctctg ggaatatctg
      961 gaaaggtatc caaggctaca ggaggtagct gagatccata gaggcattga atggaatatc
     1021 tcgctcaggg agaacaggga taggctcata tcgcccattg aaaggccaag ttttgtgcat
     1081 ggattagata ctgctcaagg gaagatagag ccattccacg ctaaaggatt tgtttatctc
     1141 aatatggacc cacagtatca gcgaaccact gcccacaagt tcgcgtggga taaaccaaag
     1201 gtcatagtca atagccacag aatgagcaga accccttggc gaatcgtcgg atatcctgat
     1261 tcgaaaggat ttgtttgtta ccagaatctc tttggaatat ggccgacaaa tgagatgaat
     1321 atcgaggtgc tggcagcgat cataaactca ccaataatca atgcggtatt cttcatccag
     1381 aattatggac gtcataattt agttgatact ctagaacaaa tacctgttcc tcttctcagc
     1441 aaagtagatg gtgcaagggt caccgacctt gtcaaacagt atagcacttc gaggcgtcaa
     1501 ttcgaaaatc cagcggacag agcgaccgct gttcaggaca gcattcgtac gctcatgaag
     1561 atcgacgctt tgatcctcaa ggcctacgat cttccgccga agcttgagcg caagctgctg
     1621 gagtttttca gggggtacaa gcgtccgttg cccttcgaat tccctgacta ttaccccgaa
     1681 gacttcaaac cctacgttcc attgtacaaa ttcattgaaa tggatttgaa acaagccagt
     1741 gccggggagc ttctcaaaag gatcacccct ctcgattcag aagaagcgca cgaccttgtg
     1801 ttgacactcg agcagaggta gcttcgccat ggatggttac ctgctggata ccaatgccgc
     1861 cagcgttcta tgggatgcca ggcatgcgga tcacgataag atcagggcgt ttctcgaatc
     1921 ggtttcacct gcgcctgtct ggatatcgat catcgcactg gcggaggttg aatacggatt
     1981 gaagacagct cccgccatga acctcgatac tcacaaggat gtgcgggctg agatggcaaa
     2041 gtatcctgag gtgctcgaac cagacaagca caccatcgcg ccttactccg atttgcgtgc
     2101 ggagctcttc aagagctatg cgccaagaga ccggaaggga agactcacca aaaagtgggt
     2161 tgaagacctc ttcgagcgca cgagtgccaa agagctccat gggagtcagg aaaacgatat
     2221 ctgggtcgca tcgctggcta tccagtacaa tcttatactc gtaaccgagg atcgcatggc
     2281 gccgattgtt gaggtgtcca agtacctgcc tgatcccctc cgaatccaga aatggagata
     2341 atcggttgca tccggttacc tcgtctctcc cgcttggaat tcccccaatc tatgatggag
     2401 taccttcaga ggatgatgaa caggcgtacc gggcagcgct ccaggttggt gcatactcga
     2461 taccggacgc ttcgaactca tgctgcagat cggtcttgag aactttgccc tcacagaaat
     2521 ccttggtgtt ggagctgagc agtacaacgg ctcccggttc ccttagaccg ctacgcattt
     2581 cgttcgacag gcgcaacgcc aattccgtta tgactgcatc cgccatactt cctttgccac
     2641 gatgggcggg cgggcgtgct tccttggtgc gcaagccggc acgtcgctcg tcctcttccg
     2701 atgcatggat actccctgcc agtgccagga actgaccgac aatctgcttc cctcgtgaaa
     2761 tcaccgtatc agcgcatttc ctcgcatcca caagttcgac gtcctgagcc tcgaaggccg
     2821 cagcgatttt ggctgcatga ttgtgacttg cccacacttt ctccaactgc tgtctcacat
     2881 cctgttcaac gccccctatc gctcgccggt attcactctg cacccaatcg caaacgacca
     2941 gatggaagca agtttttcgt gtctgcaggg catcgactat ccagagcgca ttcaccatat
     3001 cggtggggga gactctatca aacacgggag cgcggacaat gtccagcaat gaacaggtgt
     3061 ccaccaccag cactgcagat gacggggggc tttgcgtatc cataggctcg aacaccgtca
     3121 ctgtgcgtca acgtatctga gcaagtcgcg tccgaattcg tactcgatca tcaactgctg
     3181 ggctgagtcc tcgggccaat gtccgactct gaccagccac atgccgagtg tatgggcatc
     3241 cagtgagctt tcatctctga ccgtctgggc gatgaacgcg aaccagcgcc ttctatccag
     3301 tggatgagcg gagccggtcg ccttgttcgc gagattgctg aagcggagca gatggcttgc
     3361 agcttcctcg cttatccagt gagtgatcgg gagtgtatcc tctgtcagag tgcagcgcaa
     3421 attcagagcc cggcatgcag gttccgcagc ggtttcaaag aactcggcaa ggataaggtt
     3481 gtattgctgc ctcgaaagct cagagaccgt ctggggtacg atattcgtca ctgccaagtc
     3541 atcatcttgc ttcgtcataa agagactggc tgcggggatc tcgggtgtgc tgtcgcggtt
     3601 gaacaccagc caatcaaggc tctttgagcc ctcaatcaag ctgctttctt cttctctcgc
     3661 ctgatcccgc acccatccct catgcagttg cgcttcaatg acatccccga ttcgtcctat
     3721 ctcagcagcg cctccggtta tgatcagatc gcggaacacc ggcagaaaac cgtaatcgct
     3781 catggtgagc acctttctga atgaaatacc cgctccagca tggtgggcag gagggcatcg
     3841 aggtccttgc ggatggaggt gtgcttggct ttcatcatcg ctaccttgga ttggagggcg
     3901 ttaaaggaaa gctgggtgct gaggagagga acaggaaccc gtatggccat caatgagtca
     3961 gaagtaaggg tcttattacg tgcggctgtt cctggcgaag ctgcagcaat actggcaaat
     4021 ccttcttgag tggtgaaata gtaccagagg taatccgagg tagtgatatc gggcgatggc
     4081 aggcaggtta acatgcgatg gttgcctacg cagccgtgat ccgcaggttt ggcaattgct
     4141 atagcctgtt cccaagccat gatgttgcta aagatcaggt ctcctcggta tacccagaac
     4201 agtttctgcc atctaaactc tgatcccggc aaagtacgtc gatgaaaagt tcctttgtag
     4261 aaactacgca cccccagctc ggtgtaactc tgctcaggtt cgatctcgac atcacgccgc
     4321 acaaccggtg caacttcacc catagccctc cagtcggccc ttgccatgga ttcacggaat
     4381 cggattgcaa gcaatcgctc ggcatccgct tctatggcat ccagtctatc gacaacagcc
     4441 cgcgcctttg ccactaccgt ctcaagtcgg gagacgatag agcgctgtac atggatcgga
     4501 gcaagcggaa tctcgttggc aagtatttcc tccggctgaa ctcgctgccg ccgcactccg
     4561 agacctttgc tccccttggc tacagcctcc caaacacgtt tcatcgagaa gtaggcgagc
     4621 aagtagtgag gcatgaggcg ggcttcatca cagatgaatg tcgggtattc gttcgatacg
     4681 aagaagccgt caaattcatc ccccacaatc ccatacgcac cctcgaaggc aaagaggcgg
     4741 ctgtagataa actgcccttt gcgtactcgc ctcagggtcg ttgcactgct ctccagtccg
     4801 atgatcggac gctttcggaa caatccgcgc gcgtagctgt aaatgccaaa gttcgggtat
     4861 tcctggtcgc gttgaaccgg aacagtatcg ttgataagct gcaggcactc tgagagcctg
     4921 actttcggcc aatgacccat ctattatccc ctctcaccgc tccccatgat tccagcgtca
     4981 tccttcacca gagcggctat atcggcgagg agagccgccg cttcccgctc gttctcgatc
     5041 atgcgggaca acagcttttc agggggaatg tactccactg cctctctgcc gttcgggttc
     5101 ttgagatcga ggttgtagat gggccagtat acagcatcgc cttcccgttg agcgctcttg
     5161 gcggcctcct catgggcctg ccgacgggcg atgagggctt gctgttcggc ctggagagcg
     5221 gatttattgg aagacttacc ctctgcgcca ttcaaggcgg tgagcccggt gtcgatctcg
     5281 cgcaggcgcg ccgtgcattg agccgccgcc tgacgctcgc tttcggcctt cttccagaga
     5341 ggctcggcca gcgaaagagc atcggccttt atggccgcaa aatcaacccg ccaggcacgg
     5401 ccgttttcct cccgcttctt ccaccaatcg aggcacgggg caaactcctc gtattggatg
     5461 ggagcggtct tgctgtattt cttgcgcccc tctggcgggg gaagctcata gatccaaatc
     5521 tcgcgggtgg ggccgcggcg gtcgaagaag atcaggttgg cgggaatgtc ggtatagggc
     5581 gcaaataccc cctgaggcag ccgcacaatg gtgtgcaggt tgaagttctt gaggagatcc
     5641 tccttgatgc gggcgcagac tccgtctccg aaaagagtcc cgttgggtac gacgaccgca
     5701 gcccggccgg gctttccctg gctttggcgg gagcgtttga gcttgcgcat gatgagctgc
     5761 aggaagagca aagcggtttc cgatgtttgc atctcgacag gaaacctcga cttgatgccc
     5821 gtctcctcct caccgccaaa cggcggattc gtcatgatga tgtccacgcg gtccttgtct
     5881 ccgatctcct tgatcgcaac ggcaagagaa ttcccgtaga ccaggtccgg atactcgagc
     5941 ccgtggagga gaagattcat ctggctcagc atgtagggaa gaggcttcgc ttcctgcccg
     6001 aatatcgagc gctcctggag gatgcggcgc tcttcaaccg tcctgcactg accggcaaga
     6061 tgggtgtagg cctctacgag gaatccgccg gttccgcagg ccggatcgag caccatctcg
     6121 ccgagcttgg ggtcgatcac ctccaccatg aatttgacga cggggcgcgg cgtatagaac
     6181 tcaccggaat ctcccgcggc gtcccgcatc tcccgcagca tgatttcata gagatgaccg
     6241 agggtgtgga tctcctcgga tgagctgaaa tgaatcccgt ttaccttgtt tatgacgtca
     6301 cggagcaggt agccgttgat catccggttt atgacgccac ggaagacgtt tgcgatcacg
     6361 tcgcgccggt ctcggccgtt ggcggactgg agatttcgaa ggtaggcaaa aagtcccggg
     6421 cctcgactcc catccgggcg gatggcttcg tcctggttga tgaacgcaat gagctcatct
     6481 ccagtaatgc cgtcttcctt ggccgcccag tcccgccagc ggtagggtga ctcgatgacg
     6541 ggcttgaatt tcactccctc gatttgcgcc tcctgttcac ggatcatctc catgtcgtcg
     6601 aggaacttga ggaacatgat ccaggtgagc atcgggagac ggtcgagatc accgttcagc
     6661 cccttgtcct ttcgcatgat gtcacgagcg gacttgatga gagcgttcag tcgctgagcc
     6721 gtcgtttgtg gctggccgcc cgcttttgga attcttcttg ccatgctttt ctattatcct
     6781 ccatagacta cgccgcatag agctcacttt gaagcgttgc aaccgcttcg cgcagcttgt
     6841 ctgctcctcc gaagtagccg atgatttcga ccacattgcc gcggtccgag acaggcggca
     6901 ctttgagcgc atcggggatc ttgaattgac cgatgccgtg ctccgcgtat tttcccaaaa
     6961 ggtcttccag aacggctctc gcctccgggc cgtactgatc gaaaaagtcc tttctctcct
     7021 tcctcaggcg ctccgcgcgc tcgcggcggg tgcggagggg agcactgtaa gccacatggc
     7081 acagaaggtc aaagggatcg gcctcgggca gcctgagtgt ggaagcaagc tcatcgaagt
     7141 cgatccctct ttttgagagc tcctctacga tctcatcccg cccgtcggct cgcatccagt
     7201 tggatcgcag ctcgccggca gtagggtaga gcgttcggac cacatcggcg gtataatcgg
     7261 tgtatttcac aacccgaagc tgcttgccgc tttcgttgag ctcataaacc atgtgcgccg
     7321 cgatttcgat gacgccgtta tcaacgtagt actttcttgg ctcgttcggc tgcgggtcgg
     7381 tgattaccgg aggctccggg tccgatacga tctcctcatc cccgggttcc tcgctatttt
     7441 cttccggtgt cacgatttcc tcatccgtca cctgcccgct gtcatcaact gtttcttccg
     7501 tcaccagagc gggatcgccg tcgaaatcgg gatcggcaaa atggcgggta gccgaaccgg
     7561 tatagtcgag gatgctaaaa aaatactttc cgtaatcatc gcgcacacga gttccacgtc
     7621 cgatgatctg cttgaactcg gtcatggaac cgatcacacg tgccagggcc acatttttca
     7681 ccatcggagc gtccacgcct gtcgtcaaga gttgggaggt ggtgagaata acgggtgtgt
     7741 cgctctcgag ctcctgaaaa cggctgagat gcccccggcc gaccgatcct tcatcggcgg
     7801 taacccggca tgcgtaagaa ggatgatctt tgacgagatc ggtattgagg ttgttgagcg
     7861 cccttcgcat ctcttcggca tgctcctggt cgacgcagaa gacgatggtc ttggcgaagc
     7921 gatccgtctt tcgcatgaac tcagccatat gggcggcaat ggcttcggtt cgggcgcgca
     7981 gagcaacgat tctttcaaaa tctccggtct ggtactcgcc gtcaggaatc tcccgtccat
     8041 agcggtccag ctctcccttg gtgggccgcc accccaaagc gtccgcggtc gtgatcacgc
     8101 ggtgaacgcg atagggggca agaaagccgt cttcgattcc tgcacgcaag ctgtacgtat
     8161 agatgggatt tccaaagtac tggtaggtat cccggttatc ttccctgagc ggcgtcgccg
     8221 tcatgccgaa ttggacggcc ggcttgaagt actcgaggat ctctcgccag ttgctgtcgt
     8281 cgcgagcgct cccgcgatgg cattcatcga caacgatgag gtcgaaaaaa tcgggggagt
     8341 actcacggta gagccccggc cggcgttcgt cctttgcgat ggcctgatag atggcaaagt
     8401 acatctcgcg gcttttcacc acctcgccat tttcgatctt gtaccgggca tccccaaatg
     8461 gaaggaacat cttgtccttg ggatcgtcga tcagaatatt gcggtcggca agatagagga
     8521 tcttgggcct tcggtactca cccgtcgagt tccagcggct cgaccagagc ttccagcaga
     8581 cctgaaaggc aacaaaggtc tttcccgtac cggtagccat ggtgaccagg atacgctttc
     8641 tccccttgag aaccgcctgc accgtgcggt tgatggccac ttcctgatag tagcgagccc
     8701 tcttttcgaa atcgagataa aaggctgtga gcaggcgagc cgcctgttcg tcattgagcc
     8761 cctcactcat gcggagtcgc tcccagagct catcaggaga agggaatgcc tccagttctc
     8821 tttcattccc cgtgatgaag tcgaattcgc agatgccttt cccatttgtg gaataggcaa
     8881 atttaagccc cagaatctcg gcatattcct tcgcctgttc gaggccgctt gaagaagttg
     8941 catagagaga cttggcttca accacagcca gcgggaaatc acgcgtgtat ctcagcaagt
     9001 agtcggcgcg cttctgcttt ccgcgacgca ccttcccgcc gctcaccatg atgcgcccat
     9061 cggtgaaaga cctttgctcc gtgatcgaat gcggctcctc atcccacccg gcagcaacga
     9121 gctttgggac aacatacttc ctgcatgtgt cggcttcgtt cataaagtta gcgtccgcaa
     9181 aaggtaaaaa aaccagctat gtcttaacct cactgtatac cactcaacgc cccggaatga
     9241 aacaacttcg gcccgagcgc gccttcggga aagctccgac ctgcagcaaa atgctctccc
     9301 atgaaccttt tcggaaggat agtttgccct ggcttgtaga atgttcttga caggttcacg
     9361 catagtacta ttatgcaata tatcctacca atggtaggta ctaggaaagg ctgttaggaa
     9421 cagaatggaa tgacttccag tcatggattc agcttaattt ccgacatgaa gcgaggcagg
     9481 acatcagatc catgaagcca ccaagccata aaaaacaact cataagtgag cctcctgctc
     9541 tatttagtca caatagtaca ccgcgcaagt cgaaggcgtt cctctccttt tccacggcac
     9601 gaatgtgcct gcaatgcgac gccattcacc aggcgggttc atgccccaga tgcggccatc
     9661 cggaatggat gcctctgtca cgagttttgg gctgtgactg cctgaagctc agactcactt
     9721 cgtcagactg acagctcatg cacactggta tcgggacatg caagcttctt catgcgcttc
     9781 ttccggtaac gggagaagcc accaaggacc aacaatatgg ccgaaaatga aaagactctc
     9841 caagagcagc attcactgaa cgtggactct gcacaaaatc cggccggtaa ggccgcctcc
     9901 gttgaagccg ttgccgatca ccaccctggc atcaagacgt cagaagcgac tgcatcggat
     9961 agtgcgggaa atgatatgga ggatttttgc ccacgctgca tgcacccgat tgcggattcg
    10021 tcaacagcgt tttgtcccta ctgccgctgt tgcatcaact gctcatgaag actggatgaa
    10081 ttgccggcga gcattccttt gccactggcg ccatcatgag ggggaaaaac atggaagagt
    10141 acctcacggt caaggaattg tgcgaccgca taaagtacaa agaacagacg atctacaacc
    10201 tcatctcccg gggcaccttc gtcctgggac ggcatttcct caaacccact cccaagaaga
    10261 tcctcttcaa atggtcagag atcaaagcct ggatcggcga tgccgctctc tctggagaag
    10321 tcggagccgc gctggatggc cctgaaataa aggacaaaac cgcaacccag cccggccagg
    10381 tcagcctcat acgcatctaa tgaataatga aagctttctt ccgcaacgac tcccgtatct
    10441 gttactatga agacggtgaa aatcagcgga aggaggtgtt gcgatgagag gagcatagaa
    10501 tggcagaagt agcatcacat gccaatgttg tgacttttcc ccagcgcaaa aagcagtctg
    10561 agaagccgcg caaggcgggc ctaaaccgaa acagggaagg atcggtccga aggatcaacg
    10621 gcaaggtcta cgtggatttc atctatctgg gcgaaagagt gcgggagacc tcaggccttg
    10681 agtggaacga gaaaagcgcc aagcaggtgc gcgagcagct cgataagatc attgtctcga
    10741 tcaaggccgg tacgttcagg ttcgcggatg tcttcccgga gagcaagaac tgcgacttct
    10801 tcactgccaa agagcgagaa gtataccggc ttaagcaaac cccggagcag gtcgcctgca
    10861 agggtttctt tgacagctgg tatgaactgg tgaagagctc gaagcgcata actggacgca
    10921 ctctgctggg atataaaagg tatatggagc tgtatctcag cccattcttc gggagcatga
    10981 cctttgcgga cctcaacgcc cagaccttcg agaaattcat cgtttgggca aggcagcagc
    11041 actacaggaa gaaggagatt tccggcaaca ccatcaacaa gtgcttcacg atcctgaaga
    11101 tgatctgcaa aaccgtctcc atagagtatg gatggggtaa cacctacaac cccttctttg
    11161 ggttcaagaa acttccggag gatgatcctt acgaggacat tttccctttc tccgttgagg
    11221 agcagcagca gctgatcgag catctgccgg accactggaa gccctatttc cggttcgcct
    11281 tctgcgccgg actgaggccg ggcgaacaga ttgccatcaa gccggaggat atcgactgga
    11341 aacagggagt tctcaatatc cggagagcca tgacactcga tgaagagggc aaaaaagttg
    11401 tcggaagaac caagaaccgc tacagccgca gaacgataaa gctgattccg gtcatgctcg
    11461 atgcgcttca ggagcaaaag cgaatccacg accgattcca gagcgagtac ttcttctgtt
    11521 cgaccaccgg cgctcaggtg aacgtgcaca atctgcgagc gcgtgtctgg attcctgccc
    11581 taaagagagc cggcctgcaa tttcgggaga tgaagcaaac gcgccacacc ttcgcgacca
    11641 ttgcactctc gtgcggcgaa agtcctcttt ggatcgccag tgtaatgggg caccgcaaca
    11701 cggccatgat cat
//
LOCUS       DAOOVC010000120        11453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3673_length_11453_cov_5.620986, whole genome shotgun sequence.
ACCESSION   DAOOVC010000120 DAOOVC010000000
VERSION     DAOOVC010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3673_length_11453_cov_5.620986"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLM79_13500"
     CDS             <1..297
                     /locus_tag="PLM79_13500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOV87374.1"
                     /translation="LSAQPKNGNLFGHVLRTQKPLWIKHTVPPDLSPLMKPEVLALSG
                     NSPFFLMPILAKGQSIGVVYADRTPSRRELDEESFSSFCFFCHQAGLCLSYISR"
     gene            complement(287..1996)
                     /locus_tag="PLM79_13505"
     CDS             complement(287..1996)
                     /locus_tag="PLM79_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87375.1"
                     /translation="MKVALIFPPAMHPTGPPLGISLLKSFLGERNPHCSVRLWDLNLA
                     YYQKAMEWVRKEKLRIRLKGLDSAATAARVTEACDLFRGRAGIHAFLDLPRYNAAADV
                     YRRFEMVLNGLLETFSRRILVGGRVPPLAEQFFHELIEPIERDSPDLLGFSVLFSQQV
                     HFTLLLAKLLKKPSNRVVLGGATFSVMPEPRRLLGDSVSICLDSRSRGAIPVSFVDSL
                     VLGEGEWGLQGLTLDRDPDLSRVPGLVYRRDGQILSNPGRGTDNLNELPLPDFGDFPL
                     DEYHSPVPVLPYLSSRGCYWNRCAFCTHSKTYFSYREESPEKTAESLEALKDRWGVHC
                     FGLVDEMIHPGRFRRLSRRVLERELDIFYSAYAKPTGGFAPGLLDTLARSGLRVMMWG
                     VESGSQRVLDLMQKGVRKERMEKALRDSHAAGIRNLVFLMFGFPTESREEWEQTVEFV
                     AAHRECIDAVSKSRFVLLAGSRILRDPSSFGIVRILDRPRRDPVSVAYDYEVSRGLSG
                     EEADRAYANLSSVPGMDGRSPFFGIFRDHFLLHAIGPDALEKAGLPAPGRLRDAAAAP
                     VID"
     gene            complement(2016..2183)
                     /locus_tag="PLM79_13510"
     CDS             complement(2016..2183)
                     /locus_tag="PLM79_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87376.1"
                     /translation="MDSEGVTPVVVTDLHMSFGSMVWFMVKWAIAAIPACTILLVLSM
                     IPAGLFAALTR"
     gene            2751..3980
                     /locus_tag="PLM79_13515"
     CDS             2751..3980
                     /locus_tag="PLM79_13515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOV87377.1"
                     /translation="MSGKTPRGNLIEGTMIEKILIVDDSTVNRLLLSSILRKAGYETV
                     EARDGKEAVEQTFQCMPDLILLDVVMPEMDGYQVCSTLKGDDRTREIPIIFLSARTET
                     EDKIRGLDLGGADYVTKPFDKGEVLARVRNQLKIRSLTQKLIQSNRELMEKQKKLDED
                     LRAAAGIQQSLLPQKVPSIEKVEIAWRFQPCDLIGGDIFNVCRLDEANTGIYLLDVSG
                     HGVPSALVTVSVHQMLQPQQGSLVKKRKETPPYYEITSPAEVCSILDREYPIERFDKY
                     FTMVYLVLDTERGRLRYTNAGHPPPILLRGNGGLEFLDKGGPFIGLGGILPYEDEEKK
                     LDRGDKLILYTDGVVEYPNGQGDFYGEQRLVDLLGSGAGLPVGLLLDRLLESLRTFGN
                     GTPFPDDVSLLGFEIRG"
     gene            4006..4482
                     /locus_tag="PLM79_13520"
     CDS             4006..4482
                     /locus_tag="PLM79_13520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87378.1"
                     /translation="MDTDLDQTHEIGKYARTIRFIVPGRLEDVFLVGLGVRGICSSLP
                     LDPEAVYQVELSVVEAVNNAIRHAYRDREGHEVEVLVSLFPDRILFRVIDDGRGMDLE
                     STPVFDFDPEDLEGLPEGGMGLHIIRSVMDRVEYASRGGRNILTMSKAFEKQEKPG"
     gene            4546..5346
                     /locus_tag="PLM79_13525"
     CDS             4546..5346
                     /locus_tag="PLM79_13525"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV87379.1"
                     /translation="MQERRTEPRFEFTSIVLPFLGSRVEDHLCFEYIPLDISTHGLKL
                     AIPRWVVSRERLRDGDEVNLHVPFRLREGNFSRGRIVWSRWDDVHQGQVCGVRMEKAG
                     HRHCRVFFDLDTREFGIDLKQFASPEDLALKVLKDSILLKRGVLIYLNHLVPYFSRVT
                     PYPTDEYPLLKSLFLDDVKRKIEENIQKLEALHRKALEELPSGTEIPVFLDLEELRSL
                     MESEIYLDLFKITFSTEAVLPLLSAIKELEERQYVNYNTLVMLYIRSL"
     gene            complement(5361..6425)
                     /locus_tag="PLM79_13530"
     CDS             complement(5361..6425)
                     /locus_tag="PLM79_13530"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOV87380.1"
                     /translation="MIPPFVHGWLPALLVLLLPALGGVSPERAGAADRSLCWGLALEG
                     HPVTTGMIEAAEKEVGIPARIVVFFLQWPSNPEEGRFPGESLDAVRGKGAVPCITWEP
                     MYYENGREITIPHRRILEGGYDAYLASFAKAAREWGGPILLRFAHEMNLSRYHWGTSM
                     EEYGPSSPEIYRQMFRYVVSAFRKAGAANVLWAFCPNAESVPNVSFDPSSAWNEVSAY
                     YPGADSVDVVGADGYNWGTTQTREENGWDSRWRSFGEIFGPVHAQLRALAPDKPFLVF
                     ETASVVEGGDKGRWVLEAVRTAEEWRLAGITWFHVQKEQDWRFHRGLEGIRLEAVRSR
                     LKCTPGIFEGKKRMGGFRRE"
     gene            complement(6432..8057)
                     /locus_tag="PLM79_13535"
     CDS             complement(6432..8057)
                     /locus_tag="PLM79_13535"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV87381.1"
                     /translation="MPKVIRTDMVAVLTVAAMVSTFVYLIARTFLFLLGDYRWYEKVL
                     GTILLLAEIFILVHSLGYSLNIFRVATHKRSSRAFDSEVADLNSYPPVAILVASYKEP
                     LEVVRDTLTCFYNLTYPNKQLFFLDDTRYDLPGSDPEAMRAYRESVDELCRYMEVNLF
                     RRRWRGAKAGIVNDFLDFIRGNLREGFEFTSYDGKEGRERPKYLIVFDADMNPFPDFV
                     EPLVARMEQNPKLAFIQTPQYYSNFEKNRVARASGLQQAIFYEYICEGKSLEDAMFCC
                     GTNVIFRTEALLDVGGFDESSVTEDFATSLKFHVKGWSSAYLNKVCAFGMGPEDLGGY
                     FKQQFRWALGTVGLFRNIALRFFQAPRLLPMAKWWEYFLSGSHYFVGWVFLVMATCPA
                     LYLLFRIPSFFARPELYFLFFLPYFVLSLTVFLWTLHQRSYRLRDLLVGVLLNAVSFP
                     VYLKASTLAMLGVRGTFGITPKGGSTSLPLRALWPQVGLALVNFAAWIWGIQRLYYER
                     DPAAGLAMNCFWCFYHCLILSTVLFFNHPEETD"
     gene            complement(8102..>11453)
                     /locus_tag="PLM79_13540"
     CDS             complement(8102..>11453)
                     /locus_tag="PLM79_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87382.1"
                     /translation="PVGVGAVDEPRSDEDFPGAISDFEARLTLARLLSYRGDTLDEAV
                     REYRILLEERPGDPRLRAELSQVLIRRKEYPEAIRLLQRVLEANPGDLSARTALARVY
                     LWTGRNEEAVKIFEGLGREVPHPAQALEDLEAVKIFEGLGRVRNLPADVLWDMARAYT
                     WQKEYGKAEGIYELLLERPPRAARADLYTELGNVKLYSGRIDSALECYRKALEVDPDA
                     RAARKQLALALSWSGREEEALPFLAKLHERDPRDKEIAVALARSLAASGRRAEGTSLL
                     EGLAAQNPRDGELLADLADLEAGAGHAARCRELYLRALDLTEDKELLLLRYAERMNLW
                     GDFYRVESVYRDYLARHPRDSEVAVKLAGVLKSAQRYEEAEAMFLELLSRDLEREKAL
                     LGLAEVKLLEKDFQGSLAAAKRLPATDPGNQDALFLQGEALYHAGRYREALWVYQSLA
                     RAEPQKARGALAAAKTCLKLGDEESAEPYLEAASRVDPRNVEVRFYRAGPARATSNDF
                     LSTLLEPGRESPADLVEWARVYASLGYNAAAVSCFREALRQDPASFPARLGLAEVLAA
                     DHQYEPADRLLEGLGEEFPDASKILITRARVLGWGRQYDRAMEVYDRLCRLNPGDPVP
                     RKEKARTAAWGKKMGTARKVYEESYRIPVDRTLEASLEPLVRGADDPRLTKLFERLRQ
                     ASRGGSIYRGYEAFYRELAGISGSLPAPVNAGLEQVRLDLLSSYKIQKAAYLERRSKS
                     LAWDRRFTRALDSYRELIEFQPGNQEALFDYAQVECSLGLCDREAATYEKLLAVDPLH
                     LLAGMALKRQQVRSEPLVELNQAYWDEEGRGGLSRITRYRTDLNVDLPLSCRYRLTLG
                     ATQWIEDPQTGAKSFDAYGPTLKIRGLLNEYVTAAAAWTNKQYTQGDADSTHTGSVQF
                     RFNLRDYARLGLGYERTDELYNGFGIQQGLQADNFWMEASSYLARNFEVKGRAQYLNY
                     NDGNAGRVYSMVAGYEFTDHPRILKLSVTGEYRDTDREHVFHYVGNDLVDITHPYWTP
                     RDYLGTSAVLEWYHDVSKHFFCGSELHFYDLRVGAGTDTEDNPFFRFEAEWHYEFKDR
                     WVVGIKGLFHRSVQWDAAGAWATLKYRF"
BASE COUNT         2310 a         3637 c         3458 g         2048 t
ORIGIN      
        1 ctttccgcac aacccaagaa cggaaacctc ttcgggcacg tgctgcgcac gcagaagccc
       61 ctctggatca agcacaccgt cccgccggac ctatcccccc tcatgaaacc cgaggtcctt
      121 gccctgtcgg gaaattcccc cttctttctc atgccgatcc tggccaaagg ccaatccatc
      181 ggggtggtgt atgcggatcg cacacccagc cgcagggaat tggacgagga aagcttttcg
      241 agcttctgct tcttctgcca ccaggccggc ctgtgtcttt cctacatcag tcgatgacgg
      301 gagcggccgc ggcatctcga agccttcccg gggccgggag tccggctttc tcgagagcat
      361 ccgggccgat cgcgtgcagg aggaagtggt cccgaaagat cccgaaaaag ggggaccgcc
      421 cgtccatgcc cgggaccgac gaaaggttcg catacgccct gtccgcttcc tccccgctca
      481 gtccccggga cacctcgtaa tcataggcta cggaaaccgg gtcccgccgg ggccggtcca
      541 gtatccggac gattccgaag gaagaggggt cccggaggat cctggacccg gccaggagca
      601 caaatcggga cttggacacg gcatcgatgc attcccggtg agcagccacg aattccacgg
      661 tttgctccca ttcctcccgg gactcggtgg ggaacccgaa catcaggaaa accaggttcc
      721 gaatccccgc ggcgtgggag tcccgcaggg ccttttccat ccgctccttt cggaccccct
      781 tttgcatcag gtccaggacc cgctggctgc cggattccac tccccacatc atgaccctga
      841 gcccggagcg ggccagggta tcgaggagcc ccggggcaaa tcctccggtg ggcttggcgt
      901 aggcggagta aaaaatgtcc agctcccgct cgagcacccg gcggctcagg cgtcggaagc
      961 ggcccgggtg aatcatctcg tccaccagcc cgaagcaatg gaccccccat cggtctttga
     1021 gggcctccag gctttccgcc gtcttttccg gcgactcctc ccggtaggaa aaataggttt
     1081 ttgaatgggt gcaaaaggcg catcgattcc aatagcaccc ccgggaggaa agataaggca
     1141 ggacggggac gggcgagtgg tattcgtcca agggaaagtc cccgaaatcc ggcaggggaa
     1201 gctcgttcaa attatccgtt ccacgtccgg gattgctcag gatttgcccg tcccggcgat
     1261 acaccagccc ggggacccgg gagaggtccg ggtctcgatc caaggtcagg ccttgcagtc
     1321 cccattctcc ctctcccagc accagcgaat ccacaaagga aaccgggatt gcgcctcggg
     1381 accgggaatc caggcaaatg gagacggaat cccccaggag gcgccggggc tccggcatga
     1441 ccgaaaaggt cgccccgccc agcaccaccc ggttggacgg ttttttgagg agcttcgcca
     1501 ggagcagagt gaaatgaacc tgctgactaa acaggacgga aaagcccaag aggtcgggag
     1561 aatccctctc gatgggctcg atcagctcat ggaagaactg ctccgccagg ggggggaccc
     1621 tccctcccac gaggattctc ctggaaaacg tttccaggag cccattgagg accatctcga
     1681 agcgccggta tacgtccgcc gccgcgttgt agcgaggcag gtccaggaag gcgtgtatcc
     1741 cggcccggcc ccgaaagagg tcgcaggcct cggtcaccct ggccgcagtg gcggcggaat
     1801 ccagaccctt gaggcggatc cgcagcttct ccttccggac ccattccatg gccttttggt
     1861 agtaagccag gttcaaatcc cacagccgca cggaacaatg ggggttcctc tccccgagaa
     1921 aggacttcaa cagggaaatc cccaagggag gacccgttgg atgcatggcg ggcggaaaga
     1981 tcaaggcaac cttcaccggg cggctcctcg agaatctacc gggtcagagc ggcaaacaag
     2041 cccgccggga tcatgctgag gaccagcagg atcgtgcagg ccgggatcgc cgcaatggcc
     2101 cacttgacca tgaaccacac catggaaccg aaagacatgt gaaggtccgt gaccaccacc
     2161 ggggtcaccc cttctgaatc catcgaatcc ctcctgtcct ggattgatta cacggaatgg
     2221 aaccatacca accccacgtg cctttgccaa ggtccatccc tggatggagc gatttggccg
     2281 gtcggaatcc cgtttcgtca ttcccgcgca agcgggaacc cggaaagagc gttcatgccc
     2341 gccggatgcc cgccgtcgcc ggcatgacgc cctgaggctt tcggaatcgc tcaactcagg
     2401 tcatcgagtt ccccggaggc tcgctccggt ttggaccatt ctttttgggc ccttagcccg
     2461 tggagcactc gacgaacggc ggggcaccgt ccccctcgat ttcaatcccc tcccccgcca
     2521 ccctaattcc tcttccccta attccttgga ttccaggctc gacggtggta agctcccaga
     2581 gacccgggac ccgtccgtac acaggatctc ccctcgcgtc cgtcgacgcc cgccaaggca
     2641 gagggagttt cctccccgcc ctcgacaaag gccgggggag acggcccatg gagggacaag
     2701 cccgtggacc gcctgcggcc gggtccggcg aaggatcagc ctttttcccc atgagcggca
     2761 aaacgccccg gggaaacctc atcgagggaa ccatgatcga gaaaatcctc atcgtggacg
     2821 acagtaccgt gaaccggctg ttgctttcct ccattctgag aaaagccggg tatgagacgg
     2881 tggaggcccg ggacggcaaa gaagccgtgg aacaaacctt ccaatgcatg ccggacctca
     2941 tcctcctgga cgtcgtgatg cccgagatgg acggctacca ggtctgctcg accctcaagg
     3001 gggacgatcg gacccgggag atccccatca tttttctgtc cgcccgaacg gaaacggagg
     3061 acaagatccg gggcctggac ctcggggggg cggactacgt caccaagcct ttcgacaagg
     3121 gtgaggtcct ggcccgggtg agaaaccagc tcaagatccg cagcctgacc cagaagctca
     3181 tccagtccaa cagggaactg atggaaaaac agaagaagct ggacgaagac ctgagagcgg
     3241 ctgcgggaat ccagcagagt cttcttcctc aaaaggtccc gtccatcgaa aaggtcgaaa
     3301 tcgcctggcg gtttcagcct tgcgacctca tcggagggga catcttcaac gtctgtcgac
     3361 tggatgaagc gaacacggga atctatctcc tggacgtcag cgggcacggg gttcccagtg
     3421 ccctggtgac cgtttcggtg caccagatgc tccagcccca acaagggtcc ctggtcaaga
     3481 aacgaaagga aacgcctccc tactacgaaa tcacctcgcc ggcggaggtc tgcagcatcc
     3541 tggaccggga atatcccatc gaacgattcg acaagtactt caccatggtg tacctggtgc
     3601 tggatacgga aaggggccgg ttgcgctaca ccaatgccgg gcaccccccg cccatcctgc
     3661 tgcgggggaa cgggggcctc gaattcctcg acaagggagg ccccttcatc ggcctgggag
     3721 gcatcctgcc gtacgaagac gaggagaaga agctggatcg gggcgacaag ctgatcctct
     3781 acaccgacgg ggttgtggaa tacccgaacg gccagggcga cttttacggg gaacagcgac
     3841 tggtggatct cctgggcagc ggcgccggac tgcccgtcgg ccttctcctg gatcggctgc
     3901 tggaatccct gaggaccttc ggcaacggca ccccgttccc ggacgacgta agcctcctgg
     3961 gattcgaaat ccgggggtaa gagaacctaa agaccaaagg cttccatgga caccgacctc
     4021 gatcaaaccc acgagatcgg gaaatacgcc cgaaccatcc gattcatcgt gcccggcagg
     4081 ctcgaagacg tcttcctggt gggcctggga gtgcgcggca tctgctcgtc cctgcccctg
     4141 gacccggagg ccgtctacca ggtggaactg agcgtcgtcg aggcggtgaa caacgcaatc
     4201 cgccacgcct accgggaccg ggagggacac gaggtcgaag tcctcgtgag cctcttcccg
     4261 gaccggatcc ttttccgggt gatcgacgac ggccggggaa tggacctcga gagcaccccc
     4321 gttttcgatt tcgaccctga agacctcgaa ggcctgcccg aaggcggaat gggcctgcac
     4381 atcatccgat cggtcatgga ccgggtggaa tatgcaagcc ggggagggcg aaacatcctg
     4441 accatgagca aggccttcga aaaacaggaa aaacccgggt agaagaaaag ggcccggcag
     4501 acccgaaaca ggcctgccgg gggaccgcga gacccgagtg gagccgtgca ggaacgacgc
     4561 accgaaccgc gcttcgaatt cacttccatc gtacttccct tcctggggtc gagggtcgag
     4621 gaccacctgt gcttcgagta cattcccctg gacatcagca cccacggact caagctcgcc
     4681 attccccggt gggtggtcag ccgggaacga ctccgggacg gcgatgaggt caatctgcac
     4741 gtcccctttc gcctcaggga ggggaacttc agccggggga gaatcgtctg gtcgaggtgg
     4801 gacgatgtac accagggcca ggtctgcggg gtccgcatgg aaaaggcggg gcatcggcac
     4861 tgccgggtct tctttgacct cgacacccga gagttcggga tcgacctgaa gcaattcgca
     4921 tccccggagg acctggccct caaggtcctc aaggattcga tcctgctcaa acgaggggtc
     4981 ctgatctatc tcaaccacct cgttccctac ttttcgaggg tcacaccgta ccccacggac
     5041 gagtaccctt tgctgaagtc gctgttcctg gacgatgtga aaagaaagat cgaagaaaac
     5101 atccaaaagc tggaagcctt gcatagaaag gctctcgagg agctgccgtc cggaaccgag
     5161 atccccgtct ttttggacct cgaggaactg cgttccttga tggaatcgga gatctacctg
     5221 gaccttttca aaatcacctt ttccacggaa gccgtgttgc cgctcctgtc cgccattaaa
     5281 gagctcgaag agaggcaata tgttaactac aacaccctgg tgatgttgta tatccgctcc
     5341 ctgtaatcct tgaaacgcct tcattcacga cgaaatcccc ccatccgctt tttgccctcg
     5401 aagatcccgg gggtacattt cagccgggaa cgaaccgcct cgaggcggat gccctcgaga
     5461 ccccggtgga acctccagtc ctgctccttt tgcacgtgaa accaggtgat cccggccagt
     5521 ctccattcct cggcggtgcg aaccgcctcg aggacccagc gtcccttgtc tcccccttcg
     5581 acgacgctgg ccgtctcgaa aacgagaaag ggcttgtccg gggcgagggc gcgcaactgg
     5641 gcatgaacgg ggccgaagat ctccccaaag gatcgccagc ggctgtccca tccgttctct
     5701 tcccgggtct gggtggtccc ccaattgtac ccgtccgccc ccaccacgtc cacggaatcg
     5761 gcgccgggat agtatgcgct cacttcgttc caggcgctgg agggatcgaa ggacacgttg
     5821 ggcacggatt ccgcattggg gcaaaaggcc cacagcacat tggccgcccc ggccttgcga
     5881 aaagcggata ccacgtagcg aaacatctgc cggtagatct cgggactcga gggaccgtat
     5941 tcttccatgc tcgttcccca gtggtaccgg gacaggttca tctcgtgggc aaaccgaaga
     6001 aggatgggcc cgccccattc ccttgccgcc ttggcaaagg aggcgagata ggcgtcatag
     6061 cccccctcga ggatgcgccg atgcgggatg gtgatctccc gcccgttttc gtagtacatg
     6121 ggctcccagg taatgcaggg caccgcacct ttcccccgga cggcatccag gctttccccg
     6181 ggaaatctcc cttcctcggg gttggacggc cattggagga aaaacaccac gatccgcgcc
     6241 gggatcccga cctccttttc tgcggcctcg atcatccccg tcgtgacggg atggccttcc
     6301 aaggccaggc cccaacacag cgatctatcg gcggctcccg ccctctccgg gctcactccg
     6361 cccagggccg ggagaagcag aaccagcagc gccgggagcc atccgtgcac gaaaggagga
     6421 atcatggggg atcagtccgt ttcttccggg tggttgaaaa acaggacggt ggacaggatg
     6481 agacaatgat aaaagcacca gaagcaattc atggccaggc ccgcggcagg gtccctttcg
     6541 taatagagcc gctggatccc ccagatccac gcggcgaaat tcaccagggc caggccgacc
     6601 tggggccaga gcgccctgag aggcaaggac gtgctgcccc ccttgggagt gatgccgaaa
     6661 gtcccccgga cccccagcat ggccagggtc gacgccttca ggtagacggg aaagctgacg
     6721 gcgttgagca gaacgcccac aagcaggtcc cgcaatcggt aggatctctg gtggagggtc
     6781 cacaagaata cggtcaggct caggacaaag tagggtagga aaaagaggaa gtagagctcc
     6841 ggccgggcaa agaagctggg gattcgaaag aggaggtaga gcgccgggca ggtggccatc
     6901 accagaaaca cccagcccac aaagtagtga gagcccgaaa ggaagtattc ccaccacttg
     6961 gccatgggaa ggaggcgcgg ggcctgaaaa aagcgcaggg caatgtttcg aaagagcccg
     7021 acggttccca gggcccagcg gaactgctgc ttgaagtagc ctcccaggtc ttcgggtccc
     7081 atcccgaagg cacagacctt gttgagatag gcggagctcc atcccttcac gtgaaacttg
     7141 agggaagtgg cgaagtcctc cgtcaccgac gattcatcga agccccccac gtccaggagg
     7201 gcctcggtcc tgaaaatcac gttggttccg cagcaaaaca tggcatcctc gaggctcttg
     7261 ccctcgcaga tgtactcgta gaagatcgcc tgctgaaggc ccgaggcccg ggcgacccgg
     7321 tttttttcga agttcgagta gtactggggg gtctggatga aagccagctt gggattctgt
     7381 tccattctcg ccaccagggg ttccacgaaa tcgggaaagg ggttcatgtc cgcgtcgaat
     7441 acgatgaggt acttgggtct ttcccttcct tccttcccgt cataggaagt gaactcgaaa
     7501 ccctcccgga gattcccccg gatgaagtca agaaagtcgt tcacgatccc ggccttggcc
     7561 cccctccatc ttcgccggaa caggttcact tccatgtaac ggcagagctc gtcgacggat
     7621 tcccggtagg cccgcatggc ttccgggtcg ctgccgggca ggtcgtagcg ggtgtcgtcc
     7681 aggaaaaaca gctgtttgtt gggataggtc aggttgtaga aacaggtgag ggtgtcccga
     7741 acgacctcga ggggctcctt gtaggaggca accaggatcg ccacgggcgg ataggaattt
     7801 aggtcggcga cctcggagtc gaaagcccgg gaagaccgct tgtgggtggc cacccggaaa
     7861 atgttgaggg aatatcccag gctgtggacc aggataaaaa tctcggccag gagcaggatg
     7921 gtgcccagga ccttctcgta ccaccggtaa tcccccagga ggaagaggaa ggtcctcgcg
     7981 atgaggtaga caaaagtgga aaccatggcg gccaccgtaa gaacggccac catatcggtt
     8041 cgaatcacct tcggcattgc ccttggctcc ccctcccttc gccccccccg gacccggccg
     8101 gtcaaaaccg gtacttcagg gtggcccatg cgcccgcggc gtcccactgg acggatcggt
     8161 ggaacagtcc cttgatcccg accacccaac ggtctttgaa ctcgtagtgc cactcggcct
     8221 cgaaccgaaa gaacgggttg tcctcggtat cggtcccggc acccacccgc agatcataga
     8281 aatgaagctc gctgccgcaa aagaaatgct tggaaacgtc gtgataccac tcgagaacgg
     8341 cactggtgcc caggtagtcc cgaggggtcc agtagggatg ggtgatgtcc accaggtcgt
     8401 ttcccacgta gtgaaacacg tgttcccggt ccgtgtcccg gtattctccg gtaacggaca
     8461 gcttgaggat tcggggatga tccgtgaatt cataccccgc aaccatggag tagacccggc
     8521 ctgcgtttcc gtcgttgtag ttcaggtatt gcgccctgcc cttcacctcg aagtttctcg
     8581 ccaggtagga ggacgcttcc atccagaagt tgtcggcctg caggccctgc tggattccga
     8641 acccgttgta gagctcgtcg gttcgttcat agcccagtcc cagccgggca tagtcccgga
     8701 gattgaaccg gaactggacg gaacccgtgt gggtggaatc cgcatcccct tgggtatact
     8761 gcttgttggt ccaggcggcg gcggccgtga cgtactcgtt caggagcccc cggattttca
     8821 gggtgggtcc gtaggcgtcg aaggattttg ccccggtttg cgggtcttcg atccactggg
     8881 tcgcccccag ggtcaatcga taccggcacg aaagggggag atcgacgttc aaatccgtgc
     8941 ggtatcgagt gatccgggac aggcctccgc ggccttcttc atcccagtag gcttggttca
     9001 gctccaccag gggctcgctt cggacctgct gcctcttcag ggccatgccc gcgagaaggt
     9061 gcaggggatc gacggccagc agtttctcgt aggtcgccgc ttcccggtcg cagagcccca
     9121 ggctgcattc cacctgggca taatcgaaaa gggcttcctg gtttcccggc tgaaattcga
     9181 tgagttcccg gtagctgtcc agggcccggg taaacctgcg gtcccaggcc agggacttgg
     9241 agcgcctttc caggtaggcg gccttctgaa ttttgtagga ggacagcaga tccagccgca
     9301 cctgctcgag gcctgcgttc accggggcgg gcagactgcc ggaaatcccg gccagttccc
     9361 ggtagaaggc ttcgtagccc cggtagatcg atcccccccg ggaagcctga cgcagccgct
     9421 cgaagagctt tgtgagtcga ggatcgtcgg ccccgcgcac caggggttcc agggaagctt
     9481 ccagggttcg gtccacgggg atgcggtagg attcctcgta gacctttcgg gccgtgccca
     9541 tcttctttcc ccaggccgcc gtccgggctt tttcctttcg cgggacggga tccccggggt
     9601 tgagcctgca aagccggtca tagacctcca tggcccggtc gtactgcctg ccccatccca
     9661 gcacccgggc ccgggtgatg aggatcttcg aggcatcggg aaactcttcg ccgagtccct
     9721 cgaggagccg gtcggcgggt tcgtactggt gatccgcggc caggacctcg gccaggccca
     9781 agcgagcggg aaagcttgcc gggtcttgtc tcagggcctc ccggaaacac gagacggccg
     9841 cggcgttgta gcccagggag gcatacaccc gggcccactc gaccagatcg gcgggggact
     9901 cccggccggg ctccaacaga gtgctcaaaa agtcgttgga agtcgcccgg gccggacccg
     9961 cccggtaaaa gcgcacctcc acgtttcgcg gatccacccg ggaggccgct tccaggtaag
    10021 gctcggcact ctcctcgtct cccagtttca gacaggtctt ggccgcggcc agggcccccc
    10081 gggccttttg gggttcggcc cgggccaggg actggtagac ccaaagggcc tcccggtacc
    10141 gtcccgcatg atagagggcc tccccctgga ggaaaagggc gtcctggtta ccggggtccg
    10201 tggccgggag ccgtttcgcc gccgcaagag atccctggaa gtccttttcg agaagcttga
    10261 cttcggccag gcccaggagc gccttttccc gctcgaggtc ccggctcaaa agctcgagaa
    10321 acatcgcttc cgcttcctcg tagcgctggg cgctcttgag gacccccgcc agcttgacgg
    10381 ccacctccga atcccggggg tgccgagcca ggtagtcccg gtagacggat tccacccggt
    10441 agaaatcccc ccacaggttc atccgctccg cgtatcgaag gagaaggagt tccttgtctt
    10501 ccgtcaaatc caacgcccgg aggtaaagct cccgacaccg ggcggcgtgg cctgctcccg
    10561 cttccaggtc cgccagatcg gcgagcagtt ccccgtcccg ggggttttgg gcggccaggc
    10621 cttcgagcag ggacgttcct tcggcccggc gacccgaggc cgcaaggctc cgtgccaggg
    10681 ccacggcaat ttccttgtcc cgggggtccc gctcgtggag cttcgccagg aagggcaagg
    10741 cctcctcctc ccgaccggac cagctcaggg caagggccag ttgtttgcgc gccgcccggg
    10801 catcgggatc cacttccagg gcctttcggt aacattccag ggccgagtcg atccgtcccg
    10861 agtagagctt cacgttgccc aattccgtgt agagatccgc acgggctgcc cggggaggcc
    10921 tctcgagaag gagttcgtag atcccctccg ccttcccgta ttccttctgc caggtgtagg
    10981 ccctcgccat gtcccaaaga acatcggcag ggaggttccg cacccggccc aggccctcga
    11041 agatcttgac ggcttcaaga tcttcgaggg cctgggccgg gtgcggaacc tccctgccca
    11101 ggccctcgaa gatcttgacg gcttcttcgt ttctccccgt ccacagatag acccgggcca
    11161 gggccgtccg ggccgaaaga tccccgggat tggcctcgag gaccctttgc agcagccgaa
    11221 ttgcttcggg gtattctttc cgccggatca agacctggga gagctccgca cgcaaccggg
    11281 ggtccccggg acgctcctcg agaaggattc gatattcccg cacggcctcg tccagcgtat
    11341 cgccgcggta ggaaagaagc cgggcaaggg tcaagcgggc ctcgaaatcg gagattgccc
    11401 cggggaaatc ctcgtcggac cggggctcgt ccaccgctcc caccccaacc ggt
//
LOCUS       DAOOVC010000121        11450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3676_length_11450_cov_6.646424, whole genome shotgun sequence.
ACCESSION   DAOOVC010000121 DAOOVC010000000
VERSION     DAOOVC010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3676_length_11450_cov_6.646424"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLM79_13545"
     CDS             <1..613
                     /locus_tag="PLM79_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV87383.1"
                     /translation="LQEKKFERIGGTRTIQVDVGIIAATNKDLEKAVACGEFREDLYY
                     RLNVIPIRVPPLRERDGDVPLLVHHFLRRHCREKEIPLKKLSKTAAAALDQYAWPGNV
                     RELENLIERLVILTDSDEIGIEDLPPRIRQQQFQKPVSRPFPELTEEGMDLKKTLDEL
                     EDRLILDALKMAGGVKNKAAQLLGLNRTTLIEKMKKKKILYPA"
     gene            complement(750..1043)
                     /locus_tag="PLM79_13550"
     CDS             complement(750..1043)
                     /locus_tag="PLM79_13550"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOV87384.1"
                     /translation="MMPENLKRSFKQFHDEIVANGILDPKTTFMIQLGAAMAVGCYPC
                     MEILTGMAEEKGVSQEELGTVHAIVTAVSAGRVFNQYREVCRRMDVRAAAVRV"
     gene            1223..2143
                     /locus_tag="PLM79_13555"
     CDS             1223..2143
                     /locus_tag="PLM79_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOV87385.1"
                     /translation="MEIRQLRTFQTVAKFLSFNRAAQELHYAQSSISAQIQGLEEELG
                     VQLFDRLGRRVLLTEAGMRLLQYAEKILDLAEETPTGVAGARELRGSLTVKVPETLSV
                     HRLPPVIARFRSMYPKVRLRFVSCTHDGLQKDLRKGITDLAFLLAESIRASDLEVEAL
                     GIEPLVLVAHPGHPLAARKEIRARDLEGETIPLSTVDCSYRKGLELVLGEEKVRYEAA
                     LEYNSVEAVKRCVMSGVGVTVLPEISVAEEVSRGLLKVLPWADEGLEAAALMIWYKER
                     WVSPTLAAFMEVTREVLREAYGWAPRSFDG"
     gene            2184..2981
                     /locus_tag="PLM79_13560"
     CDS             2184..2981
                     /locus_tag="PLM79_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOV87386.1"
                     /translation="MESYIHGYTETEARRLSDQAHTLADLLHHDTVFSPGSRVLEVGC
                     GTGAQTVFLAGRNPGARIIAVDISPDSLEQAKDRIRREGITNVEFRQADVYRLPFGGE
                     RFDHIFVCFVLEHLARPLEALVHLKKLLKPGGSLTAIEGDHGSAYFYPGSEAAQRAIE
                     CQVRLQAGAGGNALIGRELYPLFRRAGFTEVRVSPRMVYVDASRPGLVEGFTRRTFTA
                     MIEGIREKAVGEGILDAETFDRGVRDLLRTTWEDGVFCYTFFKATGR"
     gene            3350..5698
                     /locus_tag="PLM79_13565"
     CDS             3350..5698
                     /locus_tag="PLM79_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87387.1"
                     /translation="MNRFLPVLIAVVLCGWVAGAAPLEAASGTRLQGIRFGKHPDRLR
                     VVLEAEGDRPSRIHPITDDGLMIEFKQLTVKEGLEKSLAKPPPPVSQVVFQEREGTFL
                     VKILLSSVGTSVKSFVLPRNPGYRLAFDLTGGTPAQKEDKGTEAATKAAATAGSPDKA
                     TNPRKGHFSPKIPEPASSHRTAVSVPEFPPNKETKDPAFREADELFLSSQENLAEKAE
                     GIIDLYTRAIKASPASSHIPLALYRSGLTYLAMKDPKKAQECFKKVITDFPGDAMVPQ
                     CWLSLGQIHQQGQSYVESVHALRTALRHPMEKQKMLDAHYALGRVLAGIDAHQEALEN
                     LTKCLSEDGEYYLKRPDILRSLGESYFAVQDYEKSTQYLFWYLNLEKELPDRDWILAK
                     IAEGLLYQRQFELANQVYSYIEKYHAGSEGHTIGMIRKAEFLEQQEEGLRREALEIYK
                     DLALKSLSPSLYKLVMYKLAASEWKEGNYERSLILLDETLRFKAGPGPEEKLLALRAQ
                     VLTDWVKKAHSEKDFARVVELDRQNPYTFLNSDSPDVLARIADSYCSLRLYPKALELY
                     EHLLQRSGNKNQEWIYKTAYVSYLMGETEKSLQILGRVQAESLECRKLELQGRIFFAR
                     KQYREANQTLGKILQNEKVCGPMDPEVGILYAESLMRLEKYGDALSWLPKICKLPGAD
                     PPAIRIKAGLLQGKCHQALKQPQQAIEILEELVPLLDSESQMDQVNYQLSALYLEVGQ
                     PAKAKEKLTRLLESKQELWKAAAQQQLGYIQMKSSGSKTVSF"
     gene            5766..7100
                     /locus_tag="PLM79_13570"
     CDS             5766..7100
                     /locus_tag="PLM79_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV87388.1"
                     /translation="MAVLIVEPDAQEIQAISSWIRTCGSSATATTTARSAMRMVSRDP
                     YEMILVNLNGDPSQVLALLEMSQSLADPPYVVVMSQKADLEDAVEMMKAGAHDFWVKP
                     LSGERLSRTLQWVSEKRERKVLSESSGPLPIITRNPTLLQLKSMAKRVAPSRATVFLQ
                     GESGTGKELFARYIHHHSDRRDKPFVALNCAALPETLMESELFGHEKGAFTGAIRSKE
                     GKFELAHKGTLFLDEVTEIPLHLQSKLLRVLQENEIDRVGGRHPVRVDVRVIASTNRF
                     VEETLREGRFRKDLYYRLNVIPIRIPPLRERKEDIPELCEYFMERYRVIHKCPARQIT
                     PEALALVRSHSWPGNIRELENVIQRAMLLSVGNRLDASSILLDRDPPPASADMELMPI
                     SEMEKRLIQKALASSSGNRTRAAEILGISVRTLRNKLSEYRAGEGDPGGEAE"
     gene            7589..7972
                     /gene="flgB"
                     /locus_tag="PLM79_13575"
     CDS             7589..7972
                     /gene="flgB"
                     /locus_tag="PLM79_13575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOV87389.1"
                     /translation="MDDSSGVFHRTIQAVHDRLSLNSLNQKVISGNIANLNTPGYRAK
                     ETAFEEALRESIEEQTLRLVRSRGQHLDPGDVSKARNSPQLVETGPVDLDTEMMKLAK
                     NSVEYSYMVAMLNKKFTMLRQAIAE"
     gene            7985..8422
                     /gene="flgC"
                     /locus_tag="PLM79_13580"
     CDS             7985..8422
                     /gene="flgC"
                     /locus_tag="PLM79_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOV87390.1"
                     /translation="MDFGSAMKISASGLTAHRTWMNVLSSNLANAHVTKTEAGTPYER
                     KTVIYESVPFAEQLIESLDEVPGEELERVEVREVVSDRREFKKIHDPSHPDADDQGVV
                     QMPNINPIEEMANLLMASRSYEANLAALNTAKQMALKAMDIGK"
     gene            8445..8741
                     /gene="fliE"
                     /locus_tag="PLM79_13585"
     CDS             8445..8741
                     /gene="fliE"
                     /locus_tag="PLM79_13585"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOV87391.1"
                     /translation="MRIDSVVAESLRQDPSLVKGGAQAPGPSFGEQLKAKLLEADQLQ
                     QEADKAMMDGAAKGAVNIHETMIKLEEADISLRMLVKFRNKALEAYHEVMRMQF"
     gene            8775..10370
                     /gene="fliF"
                     /locus_tag="PLM79_13590"
     CDS             8775..10370
                     /gene="fliF"
                     /locus_tag="PLM79_13590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOV87392.1"
                     /translation="MKQLLDLFQRIKTGIQAVPTPQRILYGGLFFVLVGSLGFLAYSV
                     NRTEYSPLYSRLSEEDLAAVVSTLKAKKIPYELTGNSVSVPKDSLHETRLTMASEGVP
                     KGSGVGYEIFDQQKLGSTEFVQKINYQRALQGELARTINRMNEVQESRVHLVMPTESV
                     FVADQKPPSAAVVLKLRSGARLEQQQIQGIVNLVASAVQGLNEDKVTVMSTDGQVLFK
                     KNPVDGSFHLTNLQVQLKTNIEEDLRRKVQSLLEQVVGANRVSTRISADLDFNQVRLT
                     EDTYDPDSSVIRSQQRSVESAQGGEAGAKGNPDAPVNMESQLMKSSAPGGQKTSNRQR
                     ETVNYEINHVSRQITQAPGNIKKLSVAVIVDGPYEMRPNQEGKPVPTFVGRTPEQIKS
                     IEDLVRKAVGYNESRGDQITVSNIPFATDSSGPDFVPAENRWLRMVKEHQRTLLNIVL
                     IVLAFFFVIRPFMKKFRRFAEEVIRLPEAAPAALPGQKAPLGLVELPPEPDSSTKNQA
                     IALVQRSPQQAATIIRAIMREEM"
     gene            10374..11375
                     /gene="fliG"
                     /locus_tag="PLM79_13595"
     CDS             10374..11375
                     /gene="fliG"
                     /locus_tag="PLM79_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOV87393.1"
                     /translation="MAKLSGPQKAAAILLILGESASGEILKHLTTEEIKSLGMQMASI
                     PAVKKELTDELLNEFSKRFYEEGDLYTQGEQFLRNLLPGVLGKEQANDMMSTIEQARQ
                     KIPFKFVREIDARVLANFIKSEHPQTIAIILVHLGHEKASMVLGHLPEALQFEVINRV
                     AHLETVPPDLIREVDEVLEKELLSIGEESHQLLGGVQAVAEILNFSDRRTGENILQAM
                     EDFDSDLAEKVRRLMFVFEDLVAINDSGIRELLKEVRNEELTLALKTASEELKNKVFK
                     NLSQRAVQMLEEELSIMGPVRLSDVEAAQQNILNVARRLEKEGKIMLAGRDSGDAFV"
BASE COUNT         2545 a         3304 c         3473 g         2128 t
ORIGIN      
        1 tctccaggaa aagaaattcg aaaggatcgg cgggacccgc accatccagg tggatgtggg
       61 gatcattgcc gcaaccaata aggatttgga aaaggccgtg gcctgtggtg agtttcggga
      121 ggatctctac tatcgcctga acgtgatccc cattcgagtc cctcccctga gggagcggga
      181 cggggacgtg cctctcctgg ttcaccactt cctcaggcgc cactgccggg aaaaggaaat
      241 ccccctcaaa aagctctcga agaccgccgc cgccgccctg gaccagtacg cctggcccgg
      301 caacgtgcgg gagctggaaa acctcatcga aaggctcgtc atcctcaccg attcggacga
      361 aatcgggatc gaggacctgc ctccccgcat tcggcagcaa cagttccaaa aaccggtctc
      421 ccgccccttc ccggaactga ccgaagaggg aatggacctc aagaagacct tggatgagct
      481 ggaagaccgc ctcatcctgg atgccctgaa gatggccgga ggcgtcaaga acaaggccgc
      541 ccagctgctg gggctcaacc ggaccaccct catcgaaaag atgaaaaaga aaaaaatcct
      601 ctatcccgcc tgaagccttc cgccgtttca tggacgccgc accgcgaagg tccggttccg
      661 cgaagcatgc ccgaggatga aaaccggcga accggcaccc cggttcgccg gccttcatag
      721 aatcgacggg ccgaaagacg aaacgagact cacacccgga cggccgcggc cctgacatcc
      781 atcctccggc agacttcccg gtactggttg aacaccctgc ccgcggaaac cgccgttacg
      841 atggcgtgaa cggtccccag ctcttcttgg gaaactccct tctcctccgc cattcccgta
      901 agaatctcca tgcacgggta gcagcccacc gccatggccg cccccagctg gatcatgaac
      961 gtcgtcttgg gatcaaggat tccgttggcg acgatctcgt cgtgaaactg cttgaagctc
     1021 cgtttcaggt tctcgggcat catgaggctc tccttttttc cggggttgta agctgctgtt
     1081 tgttgtcatt tctttgccgc tcgatccgaa accttgacaa ggacatgcta tcggtataat
     1141 ggaatagtag atggtaacta tcgcgtcaaa cgatttattt cgatgcaacc gatctgtttt
     1201 ttggatcgaa caaggaggtg ccgtggagat tcgtcagttg agaaccttcc agacggttgc
     1261 caagttcctc agtttcaacc gggcggccca ggagcttcat tacgcccagt ccagcatatc
     1321 ggcccagatc caggggctgg aggaggaact gggggtacag ctctttgacc ggctggggcg
     1381 gagggtccta ctgacggaag ccggcatgcg gctgctccag tacgcggaga aaatcctgga
     1441 cctggcggaa gaaaccccca caggagtggc cggagcgagg gagttgcgag gttctttgac
     1501 cgtcaaggtc cccgagaccc tcagcgtcca tcgactgcct ccggtcatcg cccgctttcg
     1561 gtccatgtat cccaaagttc gactgcgttt tgtctcctgc acccacgacg ggctgcaaaa
     1621 ggacctacgc aaaggcatta ccgacctggc ctttctgctg gccgaatcca tccgggcttc
     1681 ggacctggag gtggaagccc tgggcatcga gcccctggtg ctggtggctc accccggtca
     1741 ccccctggct gcgcgcaagg aaatccgcgc ccgggacctt gagggggaga ccattccctt
     1801 gtccacggtg gattgcagtt atcgaaaggg tctggagctg gtcctggggg aggagaaggt
     1861 gcgatacgag gcggccctgg aatacaacag cgtggaagcg gtcaaacgct gtgtcatgtc
     1921 gggggtgggg gtcacggtcc ttcctgaaat cagcgtggcg gaggaagttt cccggggact
     1981 gctcaaggtg ctgccttggg ccgatgaggg tctcgaggcg gccgccctga tgatttggta
     2041 caaggaacgg tgggtgtcgc cgaccctggc ggccttcatg gaggtgacga gggaggtgct
     2101 tcgggaagcc tacggatggg cgccacgaag ctttgacggg tagctggggg caatcacgga
     2161 cggaccatgg ggaggttcgg gaaatggaaa gctacattca cggatacacg gagacggaag
     2221 cccgacgcct ttcggaccag gcccacaccc tggccgatct gctgcatcac gacacggtgt
     2281 tttccccggg gagccgcgtc ctggaagtcg ggtgcggcac gggggctcaa acggtcttcc
     2341 tcgcgggacg gaatcccggg gcccgcatca tcgccgtgga tatttcgccc gattccctgg
     2401 aacaggccaa agacaggatc cgcagggaag ggatcaccaa tgtcgagttt cggcaagcgg
     2461 acgtgtaccg gttgccgttt ggaggggaga ggttcgatca tatctttgtc tgcttcgtcc
     2521 tcgaacacct ggccaggccc ttggaagctc tggtccatct caaaaaactg ctgaaaccgg
     2581 gcggctccct cacggccatc gaaggagacc atggttccgc gtatttttat cccgggagcg
     2641 aagccgcgca gcgggcgatc gagtgccagg ttcggctcca ggccggggct ggcggaaacg
     2701 cgcttatcgg gcgcgagctg taccctcttt tcaggcgggc cggattcacc gaagtgaggg
     2761 tctctccccg gatggtctat gtggatgcaa gcaggccggg gctggtggag gggttcaccc
     2821 ggaggacttt tacggccatg atcgaaggaa tccgggaaaa ggcggtaggg gaaggaatcc
     2881 tcgatgccga aaccttcgac cggggagtcc gggacctcct tcgcacaacc tgggaagacg
     2941 gcgttttctg ttacaccttt ttcaaggcca cgggccggta ggggaagatg gggtcctgcc
     3001 cggggatccg ggtcgtcaaa atgctgacgg agcccccgga atgcatttca tttgtcctcg
     3061 atgcccgtaa ccgggctttg atccccccga gttttccccg gtttgaacgg ggccttatct
     3121 gagaatctcc tcgtttcccc acgatgaatc ttcccgctgg cggggcgtaa cacgacgggg
     3181 tcgcggatcc cggggcgaga ctgcctcggg gggccggggg tgccccgagc tgccgtgcct
     3241 tggcacaagc attgcttttg aaggtccggg agtacgaccg ggctcacaat cctttcttac
     3301 cttgtggacg gacacgaaga tgaccgggac ggagaagaag aaccactcct tgaatagatt
     3361 cctgcctgtt ttgattgcgg tggttctgtg cgggtgggtc gcgggtgcgg ctcccctgga
     3421 agccgcttcg ggaacccggt tgcaggggat tcgatttgga aaacacccgg atcgtctgcg
     3481 tgtggtgctg gaagcggagg gagaccggcc ctcccggatc cacccgatca cggacgatgg
     3541 gctgatgatc gaattcaagc aactgacggt caaggaaggc ctcgagaaga gcctggccaa
     3601 accgccgcct ccggtgtcgc aagtggtgtt ccaggagcgg gaagggacgt tcctggtcaa
     3661 gatcctgttg tcttcggtgg ggacatcggt gaagtcgttt gtgttgcccc ggaaccccgg
     3721 ctatcggctg gcttttgatc tcaccggcgg gacacctgct caaaaggagg acaaggggac
     3781 ggaggcggca accaaggccg ccgccacggc cggttctccc gacaaggcga ccaacccgcg
     3841 gaagggccac ttttcgccaa agatccccga gcctgcttcc tcccatcgaa cggcggtttc
     3901 cgttcccgag ttcccgccga acaaggagac gaaggacccg gccttccggg aggcggacga
     3961 gctcttccta tcctcccagg agaatcttgc agagaaagcc gaagggatca tcgatcttta
     4021 cacccgggcc attaaggcgt ctcccgcctc ttcccacatt cccctggctc tctaccggag
     4081 cggtttgacc tacctggcca tgaaagaccc gaagaaagcc caggaatgct tcaaaaaagt
     4141 catcacggac tttcccggag acgccatggt gccccagtgc tggctcagcc tgggccagat
     4201 ccatcagcag ggccagtctt acgtggaatc ggtgcatgcc cttcgaacgg cgttgcgcca
     4261 tcccatggaa aagcaaaaga tgctggatgc tcactatgcc ctgggaaggg tcctggccgg
     4321 catcgacgct caccaggaag cgctggagaa cctcaccaag tgtctgagcg aggacgggga
     4381 gtactatctc aagagaccgg acattcttcg gtccctggga gaatcctact tcgcggttca
     4441 ggactatgag aagagcaccc agtacctgtt ctggtatctg aacctggaaa aggagctccc
     4501 cgaccgggat tggatcctgg cgaagatcgc cgaaggcctt ctttaccagc gccaattcga
     4561 gctggccaac caggtttatt cctacatcga gaaataccac gccgggtccg aaggacacac
     4621 catcggcatg atccgcaagg cggaattcct ggagcagcaa gaggaggggc tgcgtcggga
     4681 agcccttgaa atctacaagg acctggccct gaaatccctt tccccctccc tgtataagct
     4741 ggtcatgtac aaacttgccg cgtcggaatg gaaggagggc aactacgaaa gaagcctgat
     4801 cctgctcgat gagaccctgc ggttcaaagc agggccgggt cccgaggaaa agctgttggc
     4861 tttgagggcc caggtcctca cggactgggt gaagaaggct cattccgaga aggatttcgc
     4921 ccgggtggtg gaattggatc gtcagaaccc ctatacgttc ctcaattccg attctcccga
     4981 cgttctggcc aggattgccg acagttattg ttccctcagg ctctacccca aggccctcga
     5041 gctgtatgag cacctgctgc aaagatccgg gaacaagaac caggaatgga tctacaagac
     5101 ggcctacgtt tcctacctca tgggagagac ggaaaagagc ctccagatct tgggccgggt
     5161 ccaggccgaa tccctggaat gcaggaagct cgagctccag gggcggatct tcttcgcccg
     5221 aaagcaatac cgggaagcca accagaccct gggaaaaatc cttcagaatg aaaaggtctg
     5281 cggtcccatg gaccccgagg tggggatcct gtatgccgaa agcctaatgc ggctggaaaa
     5341 gtacggagac gccctgagtt ggcttccgaa aatctgcaag ctcccggggg ccgatccgcc
     5401 ggcaatccgg ataaaggcag gtctgttgca gggcaagtgt caccaggccc tcaagcagcc
     5461 ccaacaggcc atcgagatcc tcgaggagct ggtgcctctg ctggactcgg aatcccagat
     5521 ggatcaggtc aactatcagc tttccgctct ctacctcgag gtagggcaac cggccaaggc
     5581 caaggagaag ttgacccggt tgctcgagtc caagcaggag ctgtggaagg ccgcagccca
     5641 gcagcagttg ggctacatcc aaatgaagag ctccggctca aagaccgtgt ccttttgagg
     5701 catgggctgt ttgggggaaa ggatttgcgg gagtgccggg cgtggagtca ggtggaaggg
     5761 ggaatatggc ggtcttgatt gtagagcccg acgcgcagga gatccaggca atcagttcct
     5821 ggatccgcac ctgcggatct tccgccacgg ccaccaccac cgcccggtcg gccatgagaa
     5881 tggtctccag ggacccgtac gaaatgattc tcgtcaatct gaacggggac ccgtctcagg
     5941 ttctggccct tcttgaaatg tcccaatccc tggcggaccc tccttacgtg gtggtcatga
     6001 gtcaaaaggc cgacctcgag gatgccgtgg agatgatgaa ggccggggcg cacgatttct
     6061 gggtcaagcc cttgtcgggc gagcgcctgt cccggaccct tcaatgggtt tcggagaagc
     6121 gggaacgcaa agtcctttcg gagtcgtccg ggcctcttcc catcatcacc cgcaatccca
     6181 ccctgttgca gctgaagtcc atggcaaagc gggtggcccc cagcagggca acggtcttcc
     6241 ttcagggaga gagcggaacg ggaaaagaac tctttgcgcg gtatatccat catcacagcg
     6301 atcgccggga caagcccttc gtggccctca actgtgcggc acttcccgag accctcatgg
     6361 aaagcgagct cttcggacac gagaaagggg cttttaccgg ggccatccgg tccaaggaag
     6421 gcaagttcga actggctcac aaggggaccc tgttcctgga cgaagtcacc gagattcccc
     6481 tccatctcca gtccaagctg ctcagggtgt tgcaggaaaa cgagatcgac cgtgtgggcg
     6541 gccgtcaccc ggtccgggtt gacgtccggg tcatcgcgtc caccaaccgt ttcgtcgagg
     6601 aaacgctgag ggaggggcgg tttcgaaagg atctgtatta ccggcttaat gtaattccca
     6661 tccggattcc ccccctgcgg gagcgcaagg aagacattcc cgagttgtgc gagtatttca
     6721 tggagcggta tcgggtgatc cacaagtgtc ccgccaggca gatcactccc gaagcccttg
     6781 cccttgtccg gagccattcg tggccgggca acatacggga actggaaaac gtcattcaac
     6841 gagccatgct gctgagtgtc gggaaccgcc tggatgcgag ttccatcctc ctggaccggg
     6901 atccgccccc ggcatcggcc gacatggagc tcatgcccat cagcgagatg gaaaaacggc
     6961 tcatccaaaa ggccctggcg agctccagcg ggaaccggac gcgagccgcc gagatcctgg
     7021 gaatcagcgt tcggacgctc cgaaacaagc tgagcgagta ccgggcgggg gaaggggatc
     7081 ccgggggcga ggcggagtga gaagaatttg acgtcatcat tttgacgatt ccatgggttt
     7141 caacaccggg gaggtccacc cctcgcgagg ctccaaaacc ggcagaatag ccaatcccgg
     7201 caagcgtccg gggaacagtc ccggtgccca cctcccatga ccgaccggcg gcttgcttaa
     7261 ccctgcccgc tggccatccc aacccgggcg acagcccacc gagtctatcc cggtcctctt
     7321 ccccccaaag aagcagatag ggaaacctta tcggccactt tccccccgtg atcttccggc
     7381 cgttgccttg ccgcacccgg acagaagccg ggcccccgga ccgtcgaaga agccgaaccc
     7441 cctgtttctg gccgtccggc ggttcttgcg gggctctgct tgggagtcgt agaagccggc
     7501 ggatggggag gaaaggtccc acgtgcccgt ctggtacgag atttgcctca gggactcaat
     7561 acgaacgcat gaaaaaggag gaagcttcat ggacgactct tcaggggtgt ttcatcgaac
     7621 gattcaggcg gtgcacgacc gtctgagtct caattccttg aaccagaagg tcatttcggg
     7681 aaacatcgcc aacctcaaca ctccggggta tcgggccaag gaaacggctt tcgaagaggc
     7741 tcttcgcgag tctatcgagg aacagaccct gcgcctggtg cgttcccggg ggcaacatct
     7801 cgatcccgga gacgtgtcca aggcgaggaa ttcgccgcag ctggttgaaa cgggtccggt
     7861 ggatctcgac acggaaatga tgaagctggc caagaacagc gtggaataca gctacatggt
     7921 cgccatgctg aacaagaagt tcaccatgtt gcgacaagcc attgcggagt aaggagggct
     7981 gaaaatggat tttgggagtg ccatgaaaat cagcgcatcg ggcctgacgg cgcatcggac
     8041 ctggatgaac gtcctttcgt cgaacctggc caacgctcac gtgaccaaga cggaggcggg
     8101 aacaccctac gagaggaaga ccgtcatcta tgagagcgtc ccctttgccg agcaactgat
     8161 cgagtccctg gacgaggtcc ccggggagga attggagagg gtcgaggttc gggaagtggt
     8221 ttcggatcgg cgtgagttca agaagatcca cgatccttcc catcccgatg ccgacgacca
     8281 gggagtggtc caaatgccca acatcaaccc cattgaagaa atggccaatc tcctcatggc
     8341 gagccgttct tacgaagcga acctggcggc actgaatacc gccaagcaga tggccttgaa
     8401 ggcgatggac atcggcaaat aaccgcccat aaggagaaac cgccatgcgc atcgactcgg
     8461 tcgttgccga aagcctgcga caagatccct ccttggtgaa ggggggggcc caagcccccg
     8521 gtccgagctt cggagagcag ttgaaggcca agctgctcga ggccgatcag ctccaacagg
     8581 aggcggacaa ggccatgatg gacggagcgg ccaagggcgc cgtcaacatc cacgagacca
     8641 tgatcaaact ggaagaagcc gatatcagcc tccgaatgct ggtgaaattt cgcaacaagg
     8701 ccctggaggc ctatcacgag gtcatgagaa tgcagttcta ggcaggaacg ttttctcgaa
     8761 ggtacgggtc gtctatgaaa cagctccttg atctgttcca aagaataaaa accggcatac
     8821 aagccgttcc cactccccag agaatcctgt acggagggtt gttcttcgtc ctggtgggga
     8881 gtctcgggtt ccttgcttac agcgtcaacc gaacggaata cagtccgttg tattcgaggc
     8941 tttccgaaga agacctggcg gcggtggtct cgaccctcaa ggccaagaag atcccctatg
     9001 agctcacggg caacagtgtg tccgtcccca aggattctct tcatgaaacc cggctgacca
     9061 tggcttcgga aggagtgccc aaggggtccg gggtcggcta cgaaatcttt gaccaacaga
     9121 agctgggcag caccgaattt gtccaaaaaa tcaattatca acgggccttg cagggggagc
     9181 tggcccgcac catcaatcgc atgaacgagg tccaggaaag ccgggttcac ttggtgatgc
     9241 ccacggagag cgtcttcgtg gccgatcaga agcctcccag tgccgccgtc gttctcaaac
     9301 ttcggtcggg agcccgtttg gagcagcagc agatccaggg aatcgtcaac ctggttgcaa
     9361 gcgcagtcca gggcttgaac gaagacaaag tgacggtgat gagcacggac ggccaggtat
     9421 tgttcaagaa gaaccccgta gacggttcgt ttcacctgac caaccttcag gtgcagctaa
     9481 agaccaacat cgaagaggac ctgcgacgaa aggtccagtc tcttctcgaa caggtggtgg
     9541 gggccaatcg ggtcagcacc cgcatatccg ccgacctgga tttcaaccag gtgcgcttga
     9601 cggaagacac ttacgatccc gacagctccg tcatacgaag tcagcagcga agcgtggaga
     9661 gcgctcaggg cggagaagcg ggagccaagg ggaatccgga cgctccggtc aacatggaaa
     9721 gccagctgat gaagtcctcc gctcccggcg gacagaagac ctcgaaccgc cagcgggaga
     9781 cggtaaacta cgaaatcaac cacgtgagcc gacagatcac tcaggccccg ggaaacatca
     9841 agaagctctc ggtggcggtg attgtggacg gtccctacga aatgagaccc aaccaggagg
     9901 gcaagccggt gccgaccttt gtgggtcgaa cccccgaaca gatcaagtcc atcgaggacc
     9961 tggtgagaaa agcggtggga tacaacgaga gtcgcgggga ccagatcacg gtctccaaca
    10021 ttcctttcgc tacggattct tccggccccg atttcgtccc cgcagaaaac cggtggctga
    10081 ggatggtcaa ggagcaccag aggacccttc tgaacatcgt gctgatcgtg ctggcgttct
    10141 tctttgtcat tcgaccgttc atgaagaagt ttcggaggtt tgccgaggag gtgatccggc
    10201 ttcccgaggc cgctcctgcg gccctgcccg gccaaaaagc gcccctcggc ctggtggagc
    10261 ttcccccgga acccgacagc tcgaccaaga accaggccat tgccctggtt cagaggagtc
    10321 cccagcaggc ggcaaccatc attcgcgcca tcatgcgaga ggagatgtag tcgatggcga
    10381 aactctccgg tccccagaag gcggcagcca tccttttgat cctgggagag agtgcgtcgg
    10441 gggagatcct caagcacctc accaccgagg agatcaagag cctgggaatg cagatggctt
    10501 ccatcccggc cgtcaagaag gagctcaccg atgaactgct caatgaattc agcaaacggt
    10561 tctacgagga gggagatctc tacacccagg gagagcagtt tctgagaaac ctcctgccgg
    10621 gcgtgctggg caaggagcag gccaacgaca tgatgtccac catcgaacag gcccgtcaga
    10681 agattccctt caagttcgtg agggaaatcg atgcccgggt cttggccaac ttcatcaaga
    10741 gcgagcaccc ccaaaccatc gccatcattc tggttcacct gggacatgaa aaggcgagca
    10801 tggttctggg acatcttccc gaggccctcc agttcgaggt gatcaatcgg gtggcccacc
    10861 tggaaaccgt tccgccggac ttgattcggg aggtggacga agtcctggaa aaggagctcc
    10921 tgtccattgg ggaggagagc catcagctgc tggggggagt ccaggccgtg gccgagatcc
    10981 tgaacttttc ggaccggcga acgggagaaa atatcctgca ggccatggaa gactttgatt
    11041 ccgacctggc cgaaaaggtc cgacgcctca tgttcgtctt cgaggacctg gtggcgatca
    11101 acgactcggg gattcgcgaa cttcttaagg aggtgcgcaa cgaagaattg accttggccc
    11161 tcaaaaccgc atccgaagaa ctcaaaaaca aggttttcaa gaacctgtcc cagcgtgccg
    11221 tgcagatgct cgaggaagag ctgagcatca tgggaccggt gcggctaagc gatgtggagg
    11281 ccgctcagca gaacatcctc aacgtggcca gaaggctcga gaaggaaggg aaaatcatgc
    11341 tggcgggacg agacagcgga gacgcctttg tctaggatcc tcaagggata tcccgaagca
    11401 tccattgcct cctttgattt tcggtccatc gaaggggatg tggcggccgt
//
LOCUS       DAOOVC010000122        11444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3680_length_11444_cov_7.635262, whole genome shotgun sequence.
ACCESSION   DAOOVC010000122 DAOOVC010000000
VERSION     DAOOVC010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_11444_cov_7.635262"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3320)
                     /gene="rpoC"
                     /locus_tag="PLM79_13600"
     CDS             complement(<1..3320)
                     /gene="rpoC"
                     /locus_tag="PLM79_13600"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOV87394.1"
                     /translation="MKELYSLFTKPKDPLNFNAVKIMISSPERIREWSYGEVKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECNCGKYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHIELAASVAHIWFLRSLPSKIGNLLDLTLRELEKVLYFDSYIVLDPGETPLTKGELL
                     TEEKYRQALQEYGTGFVAGIGAEAIKQLLMDLDLEKLAAELREEMMKTNSMAKRKKLA
                     KRLKIIDAFRESENRPEWMIMDVIPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLQELNAPDIIIRNEKRMLQEAVDVLFDNGRRGKTITGASKRPLKSLSDMLKGK
                     QGRFRQNLLGKRVDYSGRTVIVIGPNLRLHQCGLPKKMALELFKPFIYNKLEEKGYVT
                     TIKAAKKMVERETPEVWDTLDEVVREFPVMLNRAPTLHRLGIQAFEPILIEGKAIQLH
                     PLVCTAFNADFDGDQMAVHVPLSIEAQAEARVLMMSTNNILSPAHGDPIIVPSQDIVL
                     GIYYMTREKPFARGEGRIFSGREEVRCAYDAGEAELHARIEVRMGNERVRTTVGRILL
                     SEILPPEIPFRLVNRIMNKKSLAQLIDQCYRKAGVKATVILADRLKDLGYEFATRSGI
                     SISIGDMVIPSRKSEILDVAFESVKEIERQYNEGLITEGEKYNKAVDIWAKATEDVAA
                     EMMREIANLELTAGSGERQKIESFNPIYMMADSGARGSKDQMRQLAGMRGLMAKPSGE
                     IIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVSQDVIISE
                     RDCNTIDGIEVEALLEAGEIIQRLSERILGRITQEDIVDPVTGDVLIPMGEEIDEKGV
                     ELVEEAGIERVPIRSVLTCRSHRGVCAMCYGRDLAQGKLVQIGEAIGIIAAQSIGEPG
                     TQLTMRTFHIGGTASKSIERTSVTNRFPGVVRFLNLNTVRNREGDLVAMNRNGEISII
                     TESGRERERYVIIYGAKLKVQDGQPVEADTLLAEWDPFTTPILTEVPGTVKFGDIAEG
                     QTMQEKLDPVTGKSSKVVVEYRDADVRPRISIKDEKGRTARVGEGGVARYFMPVGAIL
                     MVTEGDAVYPGDVLARIPR"
     gene            complement(3337..7440)
                     /gene="rpoB"
                     /locus_tag="PLM79_13605"
     CDS             complement(3337..7440)
                     /gene="rpoB"
                     /locus_tag="PLM79_13605"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOV87395.1"
                     /translation="MATALTHEYRVRKNFAKIHKIIDIPNLIEMQKESYNQFLQRDVP
                     PEARGDRGLQEVFKSVFPIEDFSGTASLEFVQYSFGEVKYDVEECLARGMTYEAPVKI
                     VVRLVVYDVDKEAGTRSIRDIKEQEIYFGTLPLMTDNGTFIVNGTERVVVSQLHRSPG
                     IFFDHDRGKTHSSGKILYSARIIPLRGSWLDLEFDPKDILYVRIDRRRKFPVTVLLKA
                     LGYTTEELLNYFYPSEKVYLGNEKQSEKELSPEILVGSRASEDILHPETGEVLIKKNR
                     KLGKQSLRRLQEVGIRRLPMKTAELVGQVLAQDVIDFETGEIVAECNDSVTESVLGEF
                     LERGIRKIELLHLEGQDVSPSFRNTLLMDKVNAREDALIEIYRRLRPSNPPTLEVATE
                     FFNNLFFNPDHYDLSEVGRLKLNLQLELDVPLDCRTLRKEDILVAVRHLIRLKDSQGP
                     VDDIDNLGNRRVRAVGELLENQYRIGLVRMERAIKERMTLQEVEALMPHDLINAKPVS
                     AVVKEFFGTSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHYGR
                     ICPIETPEGPNIGLIVSLSTYARVNPYGFIETPYMRVESGRVKKDVSYLTAMDEKDYP
                     IAQANAPVGEKGRFVNEQVSARKAGEFVMVPPEEIRYMDVSPNQLVSVSASLIPFLEH
                     DDANRALMGSNMQRQAVPLIQTRSPLVGTGIERIVAKDSGVTIVARRDGVVEYVDATR
                     IVIRATDEEPETGSGVDIYKLIKFQRSNQNTCINQKPLVHHGDVVRKGQIIADGPSTD
                     HGELALGRNVMVAFMSWGGYNFEDSILVSERIAKEDVFTSIHIEEFEVVARDTKLGKE
                     DITRDIPNVGDEALKNLDDSGIIRIGAYIKPNDILVGKVTPKGESQLTPEEKLLRAIF
                     GEKASDVKDTSLRVPPGVEGIVIDAKVFSRKGVERDDRTRMIEDMEIARLMKDQRDEL
                     DIIRKSTVKRLARLLEGKTSEGALKDGKKIYLKKGDVFTEQVLLEIPSGLWESLSVAK
                     EPALSMEIETMADNYRKQVSMVKAVFEEKINKLKRGDELPPGVIKMVKVYVAVKRKLQ
                     VGDKMAGRHGNKGVVSRILPVEDMPYFADGTPIDIVLNPLGVPSRMNVGQVLEIHLGW
                     AAKGLGQQLARMLEEHKERETLNEKLRRIYNDLEYTRYFEGASDEDLQALAADFREGV
                     HVASPVFDGADEEEIKTFLQEAGLPASGQATLYDGRTGLPFDQTVTVGVMYILKLHHL
                     VDDKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWTMEAYGAAYALQEFLTVKSD
                     DVAGRTRMYEKIVKGDNTLEAGLPESFNVLVKELQALALDVRLLEEE"
     gene            complement(7589..7975)
                     /gene="rplL"
                     /locus_tag="PLM79_13610"
     CDS             complement(7589..7975)
                     /gene="rplL"
                     /locus_tag="PLM79_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOV87396.1"
                     /translation="MCNFTKADLIQFIENMTVLELSELIKELEERFGVSAAAPMAMAV
                     MPGAAAAEAAPEEEQTEFSVVITAVGDKKIQVIKEVRAITNLGLKEAKDLVEKVPGTV
                     KEGIPKEEAEAIAKQLTEAGATVEIK"
     gene            complement(7998..8522)
                     /gene="rplJ"
                     /locus_tag="PLM79_13615"
     CDS             complement(7998..8522)
                     /gene="rplJ"
                     /locus_tag="PLM79_13615"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOV87397.1"
                     /translation="MERIQKEQVVSELRDKLQRASAAILTDFKGMSVAEMSELREALA
                     AQEVDYQVVKNTLMRLASRDTGMAVLEPHLKGTNAIAVGYRDPSVPAKILKKFNKTNE
                     KLKIKAGVLGTRLLSPEQVSTLAELPPREELLAKLLGTLNAVPTGFVTVLSGVIRSFI
                     GVLAAVKQKKEEQA"
     gene            complement(8832..9536)
                     /gene="rplA"
                     /locus_tag="PLM79_13620"
     CDS             complement(8832..9536)
                     /gene="rplA"
                     /locus_tag="PLM79_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOV87398.1"
                     /translation="MAKSGKKYTAARSKVDPARRYSFQEALQLAAEIAFCRFDETLDI
                     AVRLGVDPRHADQMVRGTVVLPNGLGKTVRVLAFAKGEKVKEAMDAGADHAGGDELVE
                     KIKEGWLDFDKTVATPDMMGTVGKIGKILGPRGLMPNAKLGTVTFDLAKVVQEIKAGK
                     VDFRVEKTGIVHAPMGKISFGGEKLLQNISAFMDTILRLKPTAAKGTYLRGIAISTTM
                     GPGIQIDPLMVKGILS"
     gene            complement(9582..10007)
                     /gene="rplK"
                     /locus_tag="PLM79_13625"
     CDS             complement(9582..10007)
                     /gene="rplK"
                     /locus_tag="PLM79_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOV87399.1"
                     /translation="MAKKVVANIKLQVSAGAANPSPPIGPALGQHGVNIMEFCKSFNA
                     KTQGQEGMIIPVVITVYADRSFTFITKTPPASILLKKTAQVAKGSSEPNRNKVAKVTK
                     AQVTEIAKLKLPDLNARDLDAAIRTIEGTARSMGIEIEG"
     gene            complement(10051..10581)
                     /gene="nusG"
                     /locus_tag="PLM79_13630"
     CDS             complement(10051..10581)
                     /gene="nusG"
                     /locus_tag="PLM79_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOV87400.1"
                     /translation="MAIKWYIVHTYSGFEHKVKTTLEERIKAEGKQALFGQILVPTEK
                     VIELVKGQRKASSRKFYPGYILVQMELNDETWHLVRHTPKVTGFIGSQERPISLSDEE
                     AQAIIQQMEEGAQKPRPKYRFEKGDEVRVVDGPFASFNGIVDEVMPEKGKVRVLVSIF
                     GRSTPVELDFVQVNRI"
     gene            complement(10685..11029)
                     /gene="secE"
                     /locus_tag="PLM79_13635"
     CDS             complement(10685..11029)
                     /gene="secE"
                     /locus_tag="PLM79_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOV87401.1"
                     /translation="MKLFQKKMGEDGDPAGKGQKAKSPKKSASLPSKPKKIEPARKAS
                     RKSDEPRFKWWKDLREYLREVVYELRKVIWPTRKETIGSTSVVLVIVILAGLFLGAVD
                     VILSRLVRFLTG"
     gene            complement(11216..11365)
                     /gene="rpmG"
                     /locus_tag="PLM79_13640"
     CDS             complement(11216..11365)
                     /gene="rpmG"
                     /locus_tag="PLM79_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOV87402.1"
                     /translation="MRVLITLACTQCKRRNYNTTKNKRTTPEKLSFRKYCRFCGAHTE
                     HKETK"
BASE COUNT         2003 a         3556 c         3203 g         2682 t
ORIGIN      
        1 cgggggatcc tcgccaaaac gtctcccgga tagaccgcat ccccttccgt caccatgagg
       61 atggccccca caggcatgaa ataacgggca actccgcctt ctcccacccg ggccgtcctc
      121 cccttttcgt ccttgatgga gatcctgggc cggacgtcgg cgtcccggta ttccaccact
      181 accttgctgg acttgcccgt cacgggatcc agcttctcct gcatggtctg tccttcggcg
      241 atgtctccga atttgaccgt ccccgggact tccgtcagga tgggggtcgt aaaggggtcc
      301 cattccgcca ggagggtatc cgcctcgacc ggttgcccgt cttggacttt gagcttggcc
      361 ccgtaaatga tcacatacct ttcccgctca cgcccggact cggtaatgat ggagatctct
      421 ccgttgcggt tcatggcaac caagtccccc tcccggttcc ggacggtatt gaggttgagg
      481 aaacgaacca cccccgggaa tcgattggtg accgaagtgc gttcgatgct cttgctggcg
      541 gtgcctccga tatggaaggt gcgcatggtg agctgggtcc ccggttctcc aatggactgg
      601 gccgcaatga tgccgatggc ctccccgatc tgcaccagct tgccctgggc cagatctcga
      661 ccataacaca tggcgcaaac ccctcgatgg cttcgacagg tcaagaccga tcggatgggc
      721 acccgttcga tccccgcttc ctccaccagt tcgacgccct tctcgtcgat ttcctccccc
      781 atgggaatca ggacgtcccc cgtcaccggg tccacgatgt cctcctgggt gattctcccc
      841 aggatgcgct cgctcaggcg ttggatgatt tcccccgctt ccaggagcgc ctccacctcg
      901 attccgtcaa tggtgttgca gtcccgctcg gagatgatga cgtcctggga cacgtccacc
      961 agtcgccggg tgaggtatcc cgaattggcc gtcttcaatg cggtatcggc caaacccttc
     1021 cgggcgccgt gagtggaaat gaagtactgg agaacggtga gtccctcgcg gaagttggcc
     1081 gtgatggggg tttcgatgat ttcccccgag ggcttggcca tgaggccgcg cattcccgcc
     1141 aactgccgca tctggtcttt gctcccccgg gctcccgagt ccgccatcat atagatggga
     1201 ttgaaggatt cgatcttttg cctttcccca ctgcctgcag tcagctccag gttggcgatc
     1261 tccctcatca tttccgcggc gacatcctcc gtagccttgg cccagatgtc caccgccttg
     1321 ttgtactttt ccccctcggt gatgagtcct tcattgtact gtcgctcgat ttccttgacc
     1381 gactcgaagg ccacatccag gatctcggat tttctcgagg ggatgaccat gtcgccgatg
     1441 gatatggaga tcccggaccg ggtggcaaac tcgtagccca ggtccttgag ccggtcggcc
     1501 aggatcaccg tggctttgac ccccgctttg cggtagcact ggtcgatgag ctgagccagg
     1561 cttttcttgt tcatgattcg gttgacaagc cggaaaggga tctcgggggg caaaatctcc
     1621 gacagcagga tccgtcccac cgtggtccgg accctttcgt ttcccatgcg gacctcgatt
     1681 cgcgcatgaa gctccgcttc cccggcatca taggcacacc gcacctcttc ccgcccggag
     1741 aagatgcggc cctctccccg ggcgaagggt ttttcccggg tcatgtaata gatgcccaga
     1801 acgatgtcct gggagggcac gatgatgggg tctccatggg cggggctcag gatgttgttg
     1861 gtggacatca tcagcacccg ggcctcggcc tgggcttcga tggacaaggg tacatggacg
     1921 gccatctggt ccccgtcgaa gtcggcgttg aaggccgtac aaaccagggg gtgaagctga
     1981 atggccttcc cctcgatgag gatgggctcg aaagcctgaa tgcccaggcg atggagggtc
     2041 ggcgcccgat tgagcatgac cgggaattcc ctcaccacct cgtccagggt atcccatacc
     2101 tccggggttt cccgttccac catcttcttg gcggccttga tggtggtgac gtagcccttt
     2161 tcctcgagtt tgttgtagat gaagggcttg aacagctcca aagccatctt cttgggaagg
     2221 ccgcactggt gcagtcgaag gttgggaccg atgacgatca ccgtacggcc cgagtagtcc
     2281 acccgtttgc ccagcaggtt ctgccggaaa cggccctgtt tgcctttgag catgtcgctc
     2341 aacgacttga gggggcgttt gctggccccg gtgatggtct tgccccgccg gccgttgtcg
     2401 aaaaggacgt ccacggcttc ctggagcatt cgcttttcat tccgaatgat gatgtccggg
     2461 gcattgagct cctgaagcct cttgagccgg ttgttgcggt tgatgaccct tcgatagagg
     2521 tcgttgagat cgctggtggc gaagcgcccg ccgtccaggg gcaccaaggg ccgcaagtcg
     2581 gggggcagaa cgggaatcac atccatgatc atccattcgg gtcgattctc cgactcccga
     2641 aaggcatcga tgatcttcaa ccgcttggcc agcttcttgc gcttggccat ggaattggtc
     2701 ttcatcattt cttcgcgaag ttccgcagcc agcttctcga gatccaggtc catgagaagc
     2761 tgtttgatgg cctcggcacc gattcccgcc acaaaacccg ttccgtattc ctggagggcc
     2821 tgacggtatt tttcctcggt gagcagctct cccttggtga gaggggtttc ccccgggtcc
     2881 aggacgatgt aggagtcgaa gtacaggacc ttctccagct cccgaagggt caggtccaga
     2941 aggttgccga tcttgctggg aagactccgg agaaaccaga tgtgggccac cgaagccgcc
     3001 agctcgatgt gtcccatgcg ctcccgccgg accttggact ggatgacttc caccccgcac
     3061 ttctcacaca ccacgccccg atgcttcatg cgtttgtact tgccgcagtt gcattcataa
     3121 tccttgatgg ggccgaagat tctcgcacag aacagcccgt cccgctcggg tttgaacgtg
     3181 cggtagttga tcgtctcggg ctttttcact tccccgtaag accattcccg gattctctcg
     3241 ggggaggaaa tcatgatctt tacggcattg aagttcagag gatctttggg cttggtgaaa
     3301 agactgtaga gctctttcaa agggacctcc ttttccttac tcttcttcca gcaatcgtac
     3361 gtccagagca agggcctgta gctcttttac caaaacattg aacgattcgg gcagaccggc
     3421 ctcgagggtg ttgtcccctt tgacgatctt ctcgtacatc cgggtcctgc ctgccacgtc
     3481 gtcggatttg acggtgagaa actcctgcag ggcgtacgcg gccccgtagg cttccatggt
     3541 ccagacttcc atttctccca gtcgctggcc tccaaactgg gccttgcctc ccagcggttg
     3601 ctgggtcacc agggagtagg ggccgatgga tcgggcgtga atcttgtcgt ccaccaagtg
     3661 gtgaagcttc aggatataca tgacgccgac ggttacggtc tggtcgaagg gaagaccggt
     3721 gcgcccatcg tacagggtgg cctgcccgga tgcgggaagg cccgcttcct gcaagaaggt
     3781 cttgatttcc tcctcgtcgg ctccgtcaaa gaccggagac gccacgtgaa ccccctcgcg
     3841 aaagtccgcc gcaagggcct ggaggtcttc gtccgaggct ccttcaaagt aacgggtgta
     3901 ttccagatcg ttgtagatcc gacgcagttt ctcgttgagg gtctcccgtt ccttgtgttc
     3961 ctccagcatt cgggccagct gctgacccag ccccttggcg gcccagccca ggtgaatttc
     4021 gagcacctgc ccgacgttca ttctcgaagg gactcccagg ggattgagca cgatatcgat
     4081 gggggtcccg tcggcgaagt acggcatgtc ttccacgggc agaatcctgg atacgacgcc
     4141 cttgttgccg tgacgacccg ccatcttgtc ccccacttgg agcttgcgct tcaccgccac
     4201 ataaaccttc accatcttga tgacgccggg cggcagttcg tcgccgcgct tgagcttgtt
     4261 gatcttctcc tcgaagaccg ccttgaccat ggagacctgc ttgcggtagt tgtccgccat
     4321 ggtctcaatc tccatggaca gggccggttc cttggcaacg ctcaggcttt cccagagccc
     4381 gctgggaatc tccagcaaga cctgctccgt gaagacgtcc cctttcttga ggtagatctt
     4441 tttgccgtcc ttgagggctc cttcgctcgt cttgccctcc aacagccggg ccaggcgctt
     4501 caccgtactt tttcggatga tgtccagctc gtcccgctgg tccttcatga gccgggcgat
     4561 ttccatgtcc tcgatcattc gggtgcggtc gtccctctcc accccctttc gggagaagac
     4621 cttggcgtcg atgacgatcc cctccactcc cggcggcacc cgcagggacg tgtccttgac
     4681 gtcgctggcc ttttccccga agatggcccg cagcagcttc tcttcgggag tcagctggga
     4741 ttcccccttg ggggtgacct ttcccaccag aatgtcgttg ggcttgatgt aagcgccgat
     4801 tcggatgatt ccgctgtcgt ccaggttctt gagggcttca tcccccacgt tggggatatc
     4861 ccgggtgatg tcttccttgc ccagcttcgt atcccgggcc accacctcaa actcttcaat
     4921 gtgaatggag gtgaaaacgt cctccttggc aatcctttca ctgacgagaa tcgagtcctc
     4981 gaagttgtag cccccccagc tcatgaaggc gaccatgacg tttctgccca gggccagctc
     5041 gccgtggtcc gtggacgggc cgtcggcaat gatctgtccc ttgcgcacca cgtccccgtg
     5101 atgcaccagg ggcttctggt tgatgcaggt gttctggttg gacctctgaa acttgatgag
     5161 cttgtagatg tccactccgc tacccgtctc cggttcctcg tcggtagccc gaatgacgat
     5221 ccgggtggcg tccacgtact ccaccacccc gtcccgccgg gccacgatgg tcaccccgct
     5281 gtccttggcc acgatgcgct cgatccccgt tcccacgaga ggggatcgag tctgaatgag
     5341 ggggacggcc tgccgttgca tgttggagcc catcagggcc cggttggcgt cgtcgtgttc
     5401 gaggaaggga atgagggagg ccgagacgct caccagctgg ttgggggaca cgtccatgta
     5461 gcggatttcc tcggggggaa ccatgacgaa ttcccccgcc ttccgggcgg acacctgctc
     5521 gttgacgaaa cgccccttct ctcccacggg agcattggcc tgggcgatgg ggtaatcctt
     5581 ttcgtccatg gcggtgaggt agctcacatc ctttttgacc cgcccggact ccacccgcat
     5641 gtagggggtt tcgatgaagc cgtaagggtt cacccgggcg tacgtggaca aggagacgat
     5701 gagaccgatg ttgggacctt ccggggtttc aatggggcag atgcgaccgt aatgggtggg
     5761 gtggacgtcc cgaacttcga acccggcccg ctcgcgggtg agaccgccgg gtcccagagc
     5821 gctcaacctg cgcttatggg tgatttccga aagaggattg gtttggtcca tgaactggga
     5881 gagctggctg gtaccgaaga actccttgac caccgcggaa acgggtttgg cattgatcag
     5941 atcgtggggc atcaaggctt ccacttcctg gagggtcatt ctctccttaa tggcccgctc
     6001 catcctcacc agcccgatgc ggtactggtt ttcgaggagt tcccccacgg cccggactcg
     6061 acggtttccc aggttgtcga tgtcgtccac gggaccctgg gagtctttca gacggatgag
     6121 gtgccggacg gcaaccagga tgtcttcctt gcgcagggtc cggcagtcca gggggacatc
     6181 gagctccagt tgcaggttga gcttcagtct tcccacttcc gaaagatcgt agtggtccgg
     6241 attaaaaaac aggttgttga agaattccgt ggccacctcg agggtgggag ggttgctggg
     6301 gcgcaatcgt cgatagattt caatgagtgc gtcttcccgg gcgttcacct tgtccatgag
     6361 gagggtgttt cggaaggaag gactgacgtc ctggccttcc aggtggagca gctcgatctt
     6421 ccggatgccc cgctccagga actctcccag gacggattcg gtaacgctgt cgttgcattc
     6481 ggcgacgatt tcgcccgtct cgaaatcgat gacgtcctgg gcaagaactt ggcccaccag
     6541 ctcggcggtc ttcatgggca ggcgccgaat ccccacctcc tgcagccgtc ggaggctttg
     6601 cttcccgagc ttccggttct tcttgatgag aacctcgccg gtctccgggt ggagaatgtc
     6661 ctcggaagcc cgacttccca ccaggatctc cgggctgagt tctttttcgg attgcttctc
     6721 gttgcccagg tagaccttct cactggggta aaagtagttg agaagctcct cggtggtgta
     6781 acccagggcc tttaggagca ccgtcacggg aaacttgcgc cgacggtcga tgcgaacgta
     6841 caagatgtcc ttggggtcaa attccagatc cagccaggaa cccctcagag gaatgatgcg
     6901 ggccgaatac aggatctttc cgctggaatg ggttttgccc cgatcatggt cgaagaagat
     6961 gcccggagac ctgtggagct ggctcaccac cactcgctcc gtcccgttga cgatgaacgt
     7021 tccgttgtcg gtcatcaggg gcagggtccc aaagtagatc tcctgttcct tgatgtcgcg
     7081 gatggatcgg gtccccgcct ctttgtccac gtcatagacc accaggcgca ccacaatctt
     7141 gacgggggcc tcgtaggtca tgccccgggc caggcattcc tcgacgtcgt acttgacctc
     7201 tccaaaactg tactgaacaa actccaggga agccgtaccg ctgaagtcct cgatgggaaa
     7261 cacactcttg aagacttcct gaagccccct gtcccctctg gcttccggtg ggacatcccg
     7321 ttgcagaaat tgattgtagc tctccttctg catttcgatg agattgggga tgtcgatgat
     7381 tttgtggatt tttgcaaaat tctttcgtac ccggtattca tgggttaagg ccgttgccat
     7441 gacgtctcct gcaactcaaa gatgggtgga gggccgtagg tcgctcctca aacagggaaa
     7501 tctcagaggg tttacaaaat caaaatcgga aaccgagctg aaggctgtcc cacagtcggt
     7561 ttccgatgca cagaggatcg gggagtagct atttgatctc gaccgtggca cccgcttcgg
     7621 tcaactgttt ggcaatcgct tccgcctctt ccttgggaat cccttccttc accgtccccg
     7681 gcaccttctc caccaggtcc ttggcctcct tgaggcccaa gttggtgatg gcccgcactt
     7741 ccttgatcac ctggatcttc ttgtcgccca ctgcggtgat caccacggag aattccgtct
     7801 gttcttcctc gggggccgct tccgccgccg ccgcccccgg catgaccgcc atggccatgg
     7861 gagccgcggc cgacacgccg aaccgctctt cgagttcctt gatcagctcg ctcagttcca
     7921 ataccgtcat gttttcaatg aattgtatca ggtccgcctt ggtgaaatta cacatcgttt
     7981 gcttcctcca taagtcctta agcttgttct tcttttttct gcttgacggc cgccagcaca
     8041 ccgatgaaag accggatcac gccgctcagg acggtcacga aaccggtcgg caccgcgttc
     8101 aaggtgccca gaagctttgc caggagttcc tcccggggag gcaactccgc cagggtcgac
     8161 acctgctccg ggctaagaag ccgagttccc aggacccctg ccttgatctt gagcttttca
     8221 ttggtcttgt tgaatttctt gaggatcttg gcgggaacgc tggggtcgcg gtagccgacg
     8281 gcaatggcgt tggtcccttt caaatggggt tccagcacgg ccatgccggt atcccgactt
     8341 gcgagccgca tcagggtgtt cttgaccacc tggtagtcca cctcctgcgc tgcaagagct
     8401 tcccgcagct cgctcatttc ggccacactc atccctttga aatcggtgag gatcgccgca
     8461 ctggctcgct gcagcttgtc gcgcagttcc gaaaccactt gctccttctg gattcgttcc
     8521 aaagtctgtc ttccctccct tcgtctgccg gcgtgctttc gtcttacgga ggtctcaccc
     8581 gggggagcgg cccgaagcct tgcggcttcc gaggacccct cgagcgaatt cacgatacat
     8641 tccgacttcc ggatctgctc atgagcccgt gagcctgatt ggttccttca accatctcgg
     8701 taggtccccc ggctttcgga ggattaaaca tctctcggat gtacctaagg tctcagatgt
     8761 ctgaaagcac caagcggcag ctagtctgtg ctcgccgcgg gaattccgag aaatccccgc
     8821 gtatctctcc cctaggacag gatccccttc accatcaggg gatcgatctg aatcccgggc
     8881 cccatggtgg tggatatggc aataccgcgc aggtaggtcc ctttggccgc cgtaggcttc
     8941 aatcgcagaa tggtgtccat gaaggccgag atattttgga ggagcttctc ccctccaaaa
     9001 gagatcttgc ccatcggggc atgaacgatc ccggtcttct ccacgcggaa atccaccttg
     9061 cccgccttga tttcctggac caccttggca agatcgaagg tcacggtgcc cagtttggcg
     9121 ttgggcatga ggccccgggg tcccaggatc ttcccgatct tgcccacggt gcccatcatg
     9181 tccggagtgg ccacggtctt gtcgaaatcc agccatcctt ccttgatctt ttccaccaat
     9241 tcgtccccgc cggcgtgatc cgcccctgcg tccatggctt ccttgacctt ctcgcccttg
     9301 gcgaaagcca gcacgcggac cgtcttgccc aacccgttgg ggaggaccac cgttcccctc
     9361 accatctggt ctgcatggcg gggatccacc cccagcctca cagcgatgtc caaggtctcg
     9421 tcaaagcggc agaaggcaat ctccgcagcc agctgaaggg cttcctgaaa gctgtatcgc
     9481 ctggccggat ccaccttgct gcgtgccgca gtatattttt tcccgctctt tgccatcttc
     9541 tagtctccaa aagtcaccct cccgttttcc tgggaggggg gctagccttc gatctcgatt
     9601 cccatgctgc gtgcggttcc ctcgatggtc cggatcgcag cgtccaggtc ccgggcgttg
     9661 aggtccggca gcttcaactt ggcgatttcc gtcacctggg ccttggtcac cttggcaacc
     9721 ttgttccggt tgggttcgct ggaacccttg gcgacctggg ccgtcttctt cagtaggatg
     9781 gaggccggcg gagtcttggt gatgaaggtg aaagatcggt ccgcatagac ggtgatgacc
     9841 accggaatga tcatcccttc ctgaccctgg gtcttggcgt tgaaggactt gcagaactcc
     9901 atgatgttga cgccgtgctg tcccaaggcc gggccgatgg gaggggaggg attcgccgct
     9961 ccggccgaca cctgcaattt gatgttcgct acaacctttt tagccattcc tgcttgaact
    10021 ccttcagaga gtctgccccg agccggggga tcagatgcgg ttcacttgca caaaatccag
    10081 ttccaccggt gtggaccggc caaagatgct cacaaggacc cgcaccttcc ctttttcagg
    10141 catcacttca tccacgatac cattgaagct ggcgaacgga ccgtccacaa cccggacttc
    10201 gtcgcccttc tcgaaccggt acttgggtcg aggtttctga gcgccttcct ccatttgctg
    10261 tatgatggcc tgagcctctt cgtccgaaag ggaaatggga cgttcctgac ttccgatgaa
    10321 cccggtcacc ttgggagtgt ggcgcaccag gtgccaggtc tcgtcgttga gttccatctg
    10381 aaccaggatg tagccgggat aaaactttcg ggaggaggcc ttccgctgtc ccttcaccag
    10441 ttcgatgacc ttctcggtcg gcacgagaat ttgtccgaaa agagcctgtt tcccttcggc
    10501 cttgatgcgt tcctccaggg tcgtcttgac cttgtgctca aatcccgagt aggtatgtac
    10561 tatgtaccac tttatggcca ccgaaatccc cattcgtcag aattcgattc gaaatcctca
    10621 agtttccctc gggcactccc ctcgggcgac aacctcattt ccccccggcc ttcccgccgc
    10681 cggtctagcc ggtgagaaac cgaaccagcc ggctcaggat cacgtccaca gcccccagaa
    10741 acagccctgc caggatcacg atcaccaaga ccacggacgt tgagccgatg gtttccttgc
    10801 gggtgggcca gatgaccttg cgcagttcat agaccacctc gcgcaggtat tccctcaggt
    10861 ccttccacca cttgaaacgc ggctcgtcgc tcttcctgga agccttcctg gcgggttcga
    10921 tcttcttggg cttcgaaggc agtgaagccg atttcttggg actcttggcc ttctggccct
    10981 tcccggccgg gtccccgtct tctcccatct tcttctggaa cagcttcacc cgattcctca
    11041 ctcccgttgt ctgcctggtt tcacaaaccc ttcaaacccg gtgcggcgct tctgcggccc
    11101 aaacccgccg tcaagggccc cgtcccggaa ggtcccgccc tcttgcggaa ttccctccac
    11161 cggcggtctc cccgtgggga ccccgccgtc ggcggccgag gccgacggga aagccctact
    11221 tggtttcctt gtgttccgtg tgggctccgc agaacctgca atatttcctg aagctcagct
    11281 tttcaggggt cgtgcgcttg ttcttggtcg tgttgtagtt gcgccgcttg cactgggtgc
    11341 acgccaaagt aatcaaaact cgcatgggga agccccttat ctcgattctt tactgaatga
    11401 tctcggtaat gaccccggcg ccgacggtgc gtccgccttc ccgg
//
LOCUS       DAOOVC010000123        11443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3682_length_11443_cov_5.854935, whole genome shotgun sequence.
ACCESSION   DAOOVC010000123 DAOOVC010000000
VERSION     DAOOVC010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3682_length_11443_cov_5.854935"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLM79_13645"
     CDS             <1..496
                     /locus_tag="PLM79_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87403.1"
                     /translation="HRSRILRQGMGIEGAVEVSGPPRFHAAFNAAPGALILPAALSML
                     RPGGRLCLFSGITDPATLQGSWVNEVHYRELTVSGAYGCTRENMTHAVELLLAHGELV
                     NLLVERTISLDQVESVFPSILAGDGFKFVVKMETPGKGLPEHGGSQPLRYKKGNHGNG
                     NAIA"
     gene            471..1550
                     /gene="trpD"
                     /locus_tag="PLM79_13650"
     CDS             471..1550
                     /gene="trpD"
                     /locus_tag="PLM79_13650"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOV87404.1"
                     /translation="MATEMQLREFGRLICRVAAGDYLTREESVEAYRQVILNEQPELQ
                     QGAFLMAHITRGPSVEELSGAWDALDRYDTAKIRTRIEGPVCDIVGTGSDSLKTINCS
                     TPAALIAAACGLPVAKKGARLVTGVSGASDIFELLGIDLNAPLSLAEKCLDQYGLCYL
                     PGEAFLKSGWARLIRSMRFTSAFNIIGPLTTPCQQTDRIVIGAYAPRVCDQLISVLKE
                     IGMKGALAPYGTATGKDPSRGMDEFSLSGPTRVVELRNGKIETYEVTPADFGLKTVDF
                     SRIASCQTAQENARAIREVLEGQTDTPRADFFCMNAAAALYISDFADTYAAGVEKAKN
                     ALASGKALEKLRQLREVQGIPGPHS"
     gene            complement(1837..3006)
                     /locus_tag="PLM79_13655"
     CDS             complement(1837..3006)
                     /locus_tag="PLM79_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV87405.1"
                     /translation="MAWFLAVFLATYTAMHLFVYSRVRALLPTDGPVRPLVLLFLLFM
                     ILAPVNARLSERSGHELLAQGIGWMGYPWMGFVFLAFWGFFFTGVLDLVFRVLGRFTP
                     VSVAPFSGRGPTAVVLLVVGLVWVYGFFEARWVRVERVTLPTSKLPAEYPRLRIAQIS
                     DVHLGILTTHGRLGKLAETIRSESPDLLVCTGDLVDGNAGSMERFAPVLRSITAPMGK
                     YAVTGNHEFYVGVEQALAALDGFGFKVLRGARETVGGVLNVAGVDDPTVGAPMDESTL
                     LSSQTNGLFTLFLKHRPEVGKGSEGLFDLQLSGHTHRGQIFPFGFFTGLIYPMQDGFY
                     RLSRGSNLYTSRGSGTWGPPLRVLSPPEVTVIDLVPVPVAGGPGNRRASFRREAP"
     gene            complement(3048..4115)
                     /locus_tag="PLM79_13660"
     CDS             complement(3048..4115)
                     /locus_tag="PLM79_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HOV87406.1"
                     /translation="MPDSKSPHLILTDRGLAAELLERLVVPILMVGSVFIFGTVGFFI
                     IGETRWSLLECAYMTSITLTTVGYGEVLEFMGANARIFAMVLMWTGMGVTLYAVSTIT
                     AFLVEKNLRTMLRERRMEKQIAALKGHYIVCGAGKTGFNVVKELRATQRACVVLDNGE
                     DRLHSIQKHFKDLFYLCGEPTEEELLRRAGIEKAAGIIAVLNDDSHNLLITVQARFIN
                     PEIKIVTRCTGENLEDKFFRAGANYVVNPAYIGGLRLASEMIRPHVVSFLDRMLRSKD
                     QSIRLEEVTLGAKSPWVGLTIGQTEVYRHTGLIPVALKHPHQEEFFYNPPQSEVLCAG
                     TVLIVIGNSTQLEKLRRYAEA"
     gene            4310..4711
                     /gene="hypA"
                     /locus_tag="PLM79_13665"
     CDS             4310..4711
                     /gene="hypA"
                     /locus_tag="PLM79_13665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOV87407.1"
                     /translation="MHEMSIAQSLLDIVLEESRRHDLASVRVVRLQIGAMAGVVPEAL
                     TFCFELLTRDTLASGALLEIETTAVVARCSGCERLVPVEDRVFLCPHCGDPLLELVSG
                     RELSVMSIEGETGNDHGSDQSSCGPEHPPGE"
     gene            4662..5330
                     /gene="hypB"
                     /locus_tag="PLM79_13670"
     CDS             4662..5330
                     /gene="hypB"
                     /locus_tag="PLM79_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOV87408.1"
                     /translation="MAQIKVPVVQNILQVNERLAAQNRKRFDDAGIFVINLMSSPGAG
                     KTSLLEKTVDALSGEFNMAVIEGDLQTACDAERIQRKGVRAIQINTEGGCHLDGNMIQ
                     IALRELDLEGLDLLVIENVGNLVCPAEFNLGEHEKVMIHSVTEGDEKPLKYPLMFQIC
                     SVLLVNKIDLLPYVDCSLEAIRRASRQLNAGQRILEISCRTGQGLESWFAWLRERIQE
                     RKKP"
     gene            5470..7800
                     /gene="hypF"
                     /locus_tag="PLM79_13675"
     CDS             5470..7800
                     /gene="hypF"
                     /locus_tag="PLM79_13675"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOV87409.1"
                     /translation="MSEGSADHHRRVLVHVHGIVQGVGFRPYLYQLAHAHRLGGWVRN
                     QSDGVEIEVSGPAPRVEDFLHDIPRKLPPLARIVAMRVEDLPFARRDGFRILGSRTRD
                     SRTALIAPDACTCEDCLRELLDPRNRRYRYPYINCTNCGPRYTIIRDIPYDREKTTMS
                     RFVMCRECREEYEDPADRRFHAQPNACWECGPRTWLTDGDGVPVAEGREAILRAVESL
                     EGGAIVAVKGLGGFHLAVRATQDEAVDRLRSRKVREEKPFAVMFPSLEAVRDYCDVNE
                     EEERLLCSSQRPIVLLNRRIEPSSPPIAPGVAPGNRFLGCFLPYTPLHHLLFHGSSFR
                     SLVMTSGNRSDEPIVMDNGDALARLRGIADRFLLHDREIYMRCDDSVTRVLLGKPRPI
                     RRARGYVPVPVFLKDSAPSVLGVGAELKSTVCLTRNRDAFLSQHIGDLENLETFRSFE
                     RTIEHLRKILEIEPELIVHDLHPDYLSTRWALSRDPLPRLAVQHHHAHIAAVIAEHRL
                     SGPVLGLAMDGTGYGPDGTVWGGEILRVEDDSWERLGHFRPLPLPGGDKAVREPWRMA
                     VSALWSLGPAGVEETVRDLRLPWPPDKLKILLQMIEKNLRSPLTSSCGRLFDAVSALI
                     GLRDAVTYEGQAAIELEQAVVPEDGAYSGRIQTVGDKKILDSLPMIAEVIQDLRSGVS
                     RGRISARFHNGLAHLLAQAAQILAEENGLDRVALSGGVFQNATLLERLVGNLTRRGLQ
                     VFAHEQVPSNDACIALGQAYIGTRRLLASTEPSNGR"
     gene            7804..8904
                     /gene="hypD"
                     /locus_tag="PLM79_13680"
     CDS             7804..8904
                     /gene="hypD"
                     /locus_tag="PLM79_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOV87410.1"
                     /translation="MKYLDEYRDPELARNLVKALQRKGSSLPRSRFMEICGTHTVAVF
                     RSGLRDLLPPSIELVSGPGCPVCVTANEDIDRVIWLAKQPNVIITTFGDLLRVPGSSS
                     SLHEQRSRGADVRMVYSTFDALQIAVDHPDREVVFIGIGFETTAPTVAAAVKQAHGKG
                     VKNFSVLSAHKLLPPAMRALLDTKDLGLDGFLCPGHVSTVIGAGAYEEIVNNYRVPCV
                     VTGFEPLDLLQGITMLADLVERGEPRVLNQYRRAVTWDGNPSARRLMAEIFEPCDAPW
                     RGLGTIPSSGLGWRSGWEQYDAARRFSMPAIEVKEHPGCRCGEVLRGKLIPPDCGLFR
                     KVCNPQSPHGPCMVSSEGTCGAYFRYHPTAEE"
     gene            9108..10118
                     /gene="hypE"
                     /locus_tag="PLM79_13685"
     CDS             9108..10118
                     /gene="hypE"
                     /locus_tag="PLM79_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOV87411.1"
                     /translation="MESKTIQLDQGSGGRATHELVRGLFVQAFRNDFLLQMNDSALVP
                     PPGGRLAVTTDSYVVDPVVFPGGNIGSLAVHGTVNDLAMLGAKPLYLTAGFILEEGLP
                     RDLLELVVFSMADAAREAGVSVVAGDTKVVQRGKADKIFINTSGIGVVPEGIRIGGQY
                     ARPGDAVILNGTIGDHGMAVLCKREGLAFENEILSDAASLYGLIGRMIETCPDIHVLR
                     DPTRGGVATTLNEIAQQSRVGIRLFEEALPVREDVIGACEMLGLDPLYVANEGKVLAC
                     VPPESARKVLEVMRSHPLGTRACVIGEVDSSDPGRVFLRTRIGGHRLVDMLRGEQLPR
                     IC"
     gene            10358..11419
                     /locus_tag="PLM79_13690"
     CDS             10358..11419
                     /locus_tag="PLM79_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV87412.1"
                     /translation="MGGGLKVCPRCIRGRWEEARPILEAVHAESRNAFRLPVRPPQNP
                     QGLQCSICLHQCRMGPGETGYCGVRDGSRQAFRHNGRTRALVSYYYDPLPTNCVADWV
                     CPGGTGAGYPQCANDPGPEVGFYNLAVFFEACNFNCLFCQNWTFKKAYGSRPNWHQVR
                     DLTQAVNGKTSCICYFGGDPTTQVPYALRMSEEARREHPRRILRICWETNGSVHPAWL
                     KRMARQSLESGGCVKVDLKAWDSRIHQALCGCDNRRVIENFARLARWIPHRPNPPFLI
                     ASTLLVPGYVEEDEVRGIASFIAGLDRDIPYTLLAFAPQFRMNDFPTTSRDHAESCLE
                     AARSAGLRRVRIGNTHLLR"
BASE COUNT         2212 a         3667 c         3492 g         2072 t
ORIGIN      
        1 ccatcgaagc cgcattcttc gccagggaat gggcatcgag ggtgccgtcg aggtttcggg
       61 acccccgcgc ttccatgcgg cgttcaatgc cgccccggga gcgttgattc tcccggcggc
      121 cctctcgatg ctgcgccccg gcgggcggct gtgcctgttc agcggcatta cggaccccgc
      181 aactctccaa ggctcctggg tcaacgaagt ccactaccgg gaactcaccg tgtcgggcgc
      241 ctacggctgc acccgggaaa acatgaccca cgccgtggag cttctcttgg ctcacgggga
      301 gcttgtgaac ctcctggtgg aaagaaccat ctccctagac caggtcgagt cggtgtttcc
      361 atcgatcctg gctggggacg ggttcaagtt cgtggtgaaa atggaaaccc cggggaaggg
      421 acttcccgag catggaggat ctcaaccctt acgctacaag aaagggaacc atggcaacgg
      481 aaatgcaatt gcgtgaattc gggcggctga tctgtcgtgt ggccgcgggg gactacttga
      541 cccgggaaga atccgtggag gcctatcgcc aggtgatcct gaacgaacag cccgaactgc
      601 agcagggggc gttcctcatg gcacacatca cccgcggacc ctccgtggaa gagctgtccg
      661 gggcctggga cgccctggac cgctacgaca ccgccaagat ccgcacccgg atcgagggac
      721 ccgtgtgcga catcgtgggc acggggtccg attccctcaa aaccatcaac tgttccaccc
      781 ccgccgccct cattgccgca gcatgcggac tgcccgtcgc caagaaaggg gcccggttgg
      841 tcacgggggt ttcgggggcc tcggacatct tcgagctcct gggaatcgac ctgaacgctc
      901 ccctctccct ggccgagaag tgcctggatc aatacggtct ctgctatctg cccggggaag
      961 cctttctcaa gtccggctgg gcacggctca tccgttccat gcgattcacg tccgcgttca
     1021 acatcatcgg tcccctcacc accccctgcc aacagaccga tcgaatcgtc atcggggcct
     1081 acgcgccccg ggtgtgcgac cagctcatct ccgttttgaa agagatcgga atgaaagggg
     1141 ccctggctcc ctatggaacg gccaccggca aggatccttc ccggggcatg gacgaatttt
     1201 ccctgtcggg tcccacccgg gtggtcgagt tgcgaaacgg caagatcgaa acctacgagg
     1261 tcactccggc ggattttggc ctgaagacgg tcgatttcag ccgcatcgca agctgccaaa
     1321 cggcccagga gaatgcccgg gccatccggg aggtactcga gggacaaacg gacaccccgc
     1381 gggccgactt cttttgcatg aacgccgcag cggccctcta tatttccgac tttgccgaca
     1441 cctacgccgc gggggtcgag aaggccaaga acgcccttgc ctcgggcaaa gctctggaaa
     1501 agctccgaca gctccgggag gtccagggaa tccccggccc ccattcttga agcccgcggg
     1561 aacacattct ccgaccggaa ctccccgggg aacgccgatc cggccattct ccgtcggaat
     1621 tccccgaagg tcatccctgt cggcatcccc ttggccgaca tccggggtcg cgcaaatcca
     1681 acggtttcca gccggaagct tgccggagag tcgagatgtg cgtgtgcatg accgtggata
     1741 ggcggcaggg gcggggtgcc ttcctgcacg tcttcgcccc gaacgcggag gccgcaggct
     1801 gggtgaatcg acctcgccgt ggtcaacctg tagttctcag ggcgcttccc gacgaaacga
     1861 tgcccgtcgg ttccccgggc ctcctgccac ggggacgggc accaggtcga tgacggtcac
     1921 ttcgggaggg gagaggaccc gcaggggagg cccccaggtg ccggagccgc ggctggtgta
     1981 gaggttcgaa ccccggctca gccggtagaa cccgtcctgc atgggataga tcaggccggt
     2041 gaagaaaccg aagggaaaga tctggcctcg atgggtgtgc ccggaaagct ggagatcaaa
     2101 cagtccttcg cttcctttcc caacttccgg gcggtgtttc aagaacaagg tgaagagccc
     2161 gttggtttgg gacgacagca gggtgctctc atccattggg gctcccacgg tgggatcgtc
     2221 caccccggct acgttcaaaa cgcctcccac cgtctccctc gccccccgca gcactttgaa
     2281 cccgaatcca tcgagggccg ccagggcctg ttccaccccg acgtagaatt catgattccc
     2341 cgtgacggcg tacttgccca tgggagccgt tatgctccgc agcacgggag cgaaccgttc
     2401 catggacccc gcgttcccgt ccaccaaatc tccggtgcac acgagcagat cgggggattc
     2461 cgagcggatc gtctcggcaa gcttccccag gcggccgtgg gtggtgagga ttcccaggtg
     2521 aacatccgaa atctgggcaa tgcgaagccg ggggtattcc gcgggaagct tcgaggtggg
     2581 aagggtcacc ctttccaccc ggacccatcg ggcttcgaaa aaaccgtaga cccataccag
     2641 ccccaccacc aggagcacga cggcggtggg accccgtccg gaaaagggag ctacggaaac
     2701 gggagtaaac cggcccagga cccggaaaac caggtccagg acccctgtga agaaaaagcc
     2761 ccaaaaagcc agaaacacaa atcccatcca cgggtagccc atccatccga tgccttgggc
     2821 caggagttca tgcccgcttc gttcgctgag gcgcgcattg acgggggcca ggatcatgaa
     2881 gaggaggaac aggagaacca gggggcgaac cggcccgtcg gtgggcagaa gagcccggac
     2941 ccgggaatag acgaacagat gcatggcggt ataggtcgcc aggaagacag cgagaaacca
     3001 ggccatcagg aggtcacatt tcttttccgg atcgtttcat ggggcaatca ggcttcggca
     3061 tagcgacgga gcttttccag ctgagtcgag ttgccgatca cgatgaggac ggttcccgca
     3121 caaagaacct cggactgggg aggattgtag aagaattcct cctggtgggg atgcttcaag
     3181 gccacgggaa tgagcccggt gtggcggtaa acttcggttt gcccgatggt caggccgacc
     3241 cagggcgact tcgcgcccag ggtgacctct tccagccgga tggattgatc cttgctccgg
     3301 agcattcgat ccaggaacga gaccacatga gggcgaatca tctcgctggc cagtcgaagc
     3361 cccccgatat aggccggatt caccacgtaa ttggcccccg cccgaaagaa cttgtcctcg
     3421 aggttttccc cggtacagcg ggtcacaatc ttgatttccg ggttgatgaa ccgagcctga
     3481 acggtaatga ggaggttgtg gctgtcgtcg ttgagaacgg cgatgatccc ggcggccttc
     3541 tcgatcccgg cccgccgcag gagctcctct tcggtgggtt ctccgcacag gtagaacaaa
     3601 tctttgaagt gcttctgaat ggaatggagg cgatcctctc cgttgtccaa caccacgcag
     3661 gcccgctggg tggcgcgcag ttccttgacc acgttgaacc cggtctttcc ggctccgcaa
     3721 acgatgtaat gtcccttcaa cgcagcaatt tgcttttcca ttctccgctc cctcagcatc
     3781 gttcgaagat tcttttccac caggaatgcc gtgatggtgg agacggcgta gagggtcacg
     3841 cccatgccgg tccacatgag caccatggca aagatacggg cattggcccc catgaattcg
     3901 aggacctcgc cgtaccctac ggtcgtgagc gtgatgctgg tcatgtaggc gcattccagc
     3961 agactccacc gggtttcccc gatgataaag aacccaacgg tgccgaagat gaaaaccgat
     4021 cccaccatca ggatgggaac cacaagacgt tccaggagct cggcggccaa cccgcggtcc
     4081 gtcaggatga gatgagggga cttgctgtcg ggcatttcgt ggaaactctc gcgtcgattc
     4141 cggattccat ccaactacgt caaccaactc cccgggagac ccgccccaat tttcagccag
     4201 agcagcatgc gattttccgg gacgggagtc aagaggatcg tttccggtca gattgccaag
     4261 gagaaattcc aatgttatgt agagaacact ttgcagacag ggagtcccaa tgcacgagat
     4321 gtccatcgct caaagccttc tggacattgt tctcgaggaa agccgcaggc acgatctcgc
     4381 gtcggttcgg gtggttcgtc tgcagatcgg ggccatggcc ggggttgtgc ccgaggcttt
     4441 gaccttctgc ttcgagctct tgacccggga caccctcgca tcgggggccc ttttggagat
     4501 cgagaccacc gccgtcgtgg ctcgatgctc cggctgtgaa aggctcgtcc cggtggaaga
     4561 ccgggtcttt ttgtgccccc attgcggaga tcctctgctg gagttggtga gcgggagaga
     4621 actatccgtt atgagcattg aaggggaaac gggaaacgac catggctcag atcaaagttc
     4681 ctgtggtcca gaacatcctc caggtgaatg aacgtctggc cgctcagaac cgcaaacgct
     4741 tcgacgatgc gggcatcttc gtcatcaacc tcatgagctc gcccggcgca ggcaagacgt
     4801 ctcttctgga aaaaaccgtg gacgccctct cgggcgaatt caacatggcg gtgatcgagg
     4861 gagatcttca gaccgcctgc gatgccgaac gcatccagcg caaaggggtc cgggcgattc
     4921 agatcaacac cgaagggggc tgtcatctcg acggcaacat gatccagatc gccttgcggg
     4981 aactggacct cgaggggttg gacctgctgg tcatcgaaaa cgtcggcaac ctggtatgcc
     5041 ctgccgagtt caacctgggg gaacatgaga aggtcatgat tcacagtgtg accgaggggg
     5101 acgaaaaacc cctgaaatat cccctgatgt tccagatctg cagtgttctt ctggtcaaca
     5161 agatcgacct gctcccctac gtggactgca gcctggaagc gatccgccgg gcaagccgcc
     5221 aactgaatgc cggacaaaga atccttgaaa tctcctgccg cacaggccag ggactcgagt
     5281 cgtggttcgc ctggcttcgg gagcgcatcc aggagcggaa gaaaccttga ggcccgagtg
     5341 ctccccccaa gggtgccgca accggacccc gggtccccac cctcctcggt cgggcccgat
     5401 cgtaagccgg ggtcggtttt ccgggagccc cccgaaacaa cgattttacg agcagcacga
     5461 ggcagtcgag tgagtgaagg ttccgcggac catcatcgac gggtcctggt tcacgtccac
     5521 ggcatagtcc agggagtcgg ttttaggccg tacctgtatc agctggccca tgcgcaccgg
     5581 ctgggcgggt gggtgcgaaa ccagtccgac ggggtcgaga tcgaggtttc gggacccgct
     5641 ccccgtgtgg aggattttct tcacgacatc ccgcggaagc tcccccccct ggcccggatc
     5701 gtggccatgc gggtcgagga cctgccgttc gcccgccggg acgggttccg catcctcggg
     5761 agtcggaccc gggattcccg taccgccctc attgcccccg acgcctgcac ctgtgaggac
     5821 tgcctccggg aactcctgga tccccggaat cgacgctacc gctatccata catcaattgc
     5881 accaattgcg gtccccggta caccatcatc cgggacattc cctacgaccg ggaaaagacg
     5941 accatgtccc ggttcgtcat gtgccgggaa tgccgggaag agtacgagga cccggccgac
     6001 cgcaggtttc acgcccagcc caatgcctgc tgggaatgcg gtccacgcac gtggctgacc
     6061 gacggggacg gcgtccccgt ggccgaaggg cgggaagcga ttcttcgggc tgtcgagtcc
     6121 ctcgaggggg gggccattgt ggccgtcaag gggctgggag gcttccatct ggcggtccga
     6181 gccacccaag acgaggccgt ggatcgcctt cgatcgagga aagtccgcga agaaaaacct
     6241 tttgccgtga tgttcccgag cctcgaagcc gttcgggatt actgcgatgt caacgaggag
     6301 gaggaaagac tcctgtgcag ttcccagcgt cccattgtac ttctgaaccg gcgcatcgag
     6361 ccctcgtccc cgcccatcgc tccgggggtt gctcccggaa atcgcttcct gggatgcttt
     6421 ttgccttaca ctcccctgca tcatctcctg tttcacggca gctcgtttcg atccctggtc
     6481 atgaccagcg gcaaccgcag cgacgagccc atcgtcatgg acaacgggga cgccctggca
     6541 cgtctccggg gaattgccga ccgattcctg cttcacgacc gcgagatcta catgcgctgt
     6601 gacgattcgg tcacccgggt ccttctcggg aagccccgac ccattcgtcg cgcccggggc
     6661 tatgtccccg tgccggtgtt tctcaaggat tcagccccct cggtgctggg ggtgggagcc
     6721 gagctcaaga gcaccgtgtg cctcacccgg aaccgggatg ccttcctcag ccagcacatc
     6781 ggggacctcg aaaacctcga gacctttcgg tccttcgagc ggaccatcga gcatctgcgc
     6841 aagatcctcg agatcgaacc cgagctcatc gtccacgacc tacacccgga ctacctgagc
     6901 acccggtggg ccctttcccg ggaccctctg ccccgattgg ccgtgcagca ccaccatgcc
     6961 cacatcgccg cggtgatcgc cgaacaccgg ctctcgggac ccgtcctggg gctggccatg
     7021 gacggcaccg gctacggccc cgacggaacc gtctgggggg gcgagattct ccgggtggaa
     7081 gacgattcat gggaacgact gggacatttc cgccccctgc ctctcccggg aggcgacaag
     7141 gccgtgcggg agccgtggcg gatggcggtt tccgccctgt ggagcctggg tcccgccgga
     7201 gtggaagaaa cggttaggga tttgcggctc ccatggcccc cggacaaatt gaagatcctc
     7261 cttcagatga tcgaaaagaa cctccgttcc cccctcacct cctcttgcgg acgtctcttt
     7321 gacgcggttt ccgccctcat cggcctgcgg gacgccgtca cctacgaagg ccaggccgcc
     7381 atcgagctcg agcaggccgt cgtgcccgaa gacggcgcct attcgggccg gatccaaacc
     7441 gtaggggaca agaagatcct ggattccctg cccatgatcg ccgaggtgat ccaagacttg
     7501 agatccgggg tttcccgggg ccggatctcc gcccggttcc acaacgggct ggcccacctg
     7561 ctcgcccaag ccgctcaaat cctggccgag gaaaacgggc tcgatcgagt ggccctgagc
     7621 ggaggggtct ttcagaatgc aaccctgctg gaacgcctgg tgggaaatct cacccgccgg
     7681 ggcctccaag tctttgccca cgagcaagtc ccctccaacg acgcctgcat tgccctgggc
     7741 caggcctaca tcgggactcg acgactcctg gcatccaccg aaccgtccaa cgggagatga
     7801 gacgtgaaat acctggatga atatcgcgac ccggaactgg cccggaatct cgtgaaagcc
     7861 cttcagcgaa aaggttcttc cctgccccgg tcccggttca tggagatctg cgggacgcac
     7921 accgtggccg tctttcgctc cggcctgagg gaccttctgc cgccttccat cgagctcgtc
     7981 agcggccccg gatgccccgt ctgcgtgacc gccaacgagg atatcgaccg ggtcatctgg
     8041 ctggcgaaac agcccaacgt catcatcacc accttcggag acctcttgag ggttcccggc
     8101 tcttcctcct ccctccatga acaacggagc cggggagccg acgtgcgaat ggtctattcc
     8161 accttcgacg ctcttcaaat cgctgtggat catcccgacc gcgaggtggt gttcatcggc
     8221 atcgggttcg agaccaccgc ccccaccgtg gcggcggccg tcaagcaagc ccacggcaaa
     8281 ggggtcaaga acttctcggt gctctccgcc cacaagctcc tcccccccgc catgcgcgcg
     8341 ctcctggaca ccaaagacct gggtctcgac ggcttccttt gtcccggtca cgtcagcacc
     8401 gtcatcggcg caggcgccta cgaggaaatc gtgaacaact accgggtgcc ctgcgtggta
     8461 accggtttcg aacccctgga tcttctccag gggatcacca tgctggcgga cctggtggaa
     8521 cggggggagc cccgggtgtt gaaccagtac cggcgggccg taacctggga cggcaatcct
     8581 tcggcccggc ggctcatggc agagatcttc gaaccctgcg acgccccgtg gcgagggttg
     8641 ggaaccattc cttccagcgg gttgggatgg cgttccggct gggagcaata cgacgccgcc
     8701 cgccgctttt ccatgcccgc catcgaggtg aaggaacacc cgggatgtcg gtgcggggag
     8761 gtccttcgag ggaaattgat ccctcccgac tgcggcctct tccgaaaagt ctgcaacccc
     8821 caatcccctc acgggccctg catggtttcc agcgagggca cttgcggagc ctacttccgg
     8881 tatcacccga ccgccgagga gtgagggacg acggcaccca aaaggaaagg accccccgag
     8941 tcgaacgatt tggccggcac caatcccgct tcgtctttcc cgcccaggcg gggacccata
     9001 aagagcgtgc ccatcctccg gatggccgcc ttggcgggca ttacgggcca aggctatgaa
     9061 aatcgctcaa atgagggatc cgtcattggc agaagctgga ggaatccgtg gaatcgaaaa
     9121 ccatacagtt ggatcaaggc agcggggggc gcgccaccca cgaactggtg cggggtctct
     9181 tcgttcaggc ctttcgcaac gatttcctgc tccagatgaa cgacagcgcc ctggttccgc
     9241 cccccggggg cagactggcc gtcaccaccg actcgtacgt ggtggacccc gttgtctttc
     9301 cgggaggaaa catcggcagc cttgccgtcc atgggaccgt gaacgacctg gcaatgctcg
     9361 gcgccaaacc cctctacctg accgcgggct ttatcctcga ggaaggcctg ccccgggacc
     9421 tcctcgagct ggtggtgttt tccatggccg atgccgcccg ggaagccggg gtatccgtcg
     9481 tggccggaga caccaaggtg gtccagcggg gaaaagcgga caagatcttc atcaatacct
     9541 cggggatcgg cgtggttccg gaagggattc gcatcggcgg acaatacgcc cgccccgggg
     9601 atgccgtgat cctcaacggc accatcgggg accacggaat ggccgtgctc tgcaaacgag
     9661 aagggctggc cttcgaaaac gagatcctca gcgacgccgc gtccctatac ggcctcatcg
     9721 gccggatgat cgaaacctgc ccggatatcc atgtgcttcg agaccccacc cggggaggcg
     9781 tcgccaccac cctgaacgaa atcgcccagc agtcccgcgt gggaattcgg ctcttcgagg
     9841 aggctcttcc cgtgcgggaa gacgtgatcg gcgcctgcga aatgctcggg ctggaccccc
     9901 tctacgtggc caacgaagga aaggtcctcg cctgcgttcc cccggaatcc gcccggaagg
     9961 tcctcgaggt catgcgctcc cacccgctgg gaacccgggc ctgcgtcatc ggagaagtgg
    10021 actcgtcgga cccgggacgc gtgtttctcc gaacgcgcat cggaggtcat cgcctggtgg
    10081 acatgttgcg gggagagcaa ctgccgcgca tctgctgatt cgatcccgcc cgggggcgat
    10141 gggccggggg caaggggccg gggcccgggg caaggttgaa aacccgcggc cgggaatggg
    10201 aatgaaatga agttggacgg tacggcggtt tctcttggga ttttggggta ccgggatttt
    10261 tccgggagcg ggttgaagcg atatgtggat tgaacgattg gggaagactc ggggttccgg
    10321 cttgacctgc tcggtttgcg gggaaaccgt cccgggaatg ggtggaggcc tgaaggtctg
    10381 cccccggtgc atccgcgggc gatgggaaga ggcgcggccg attctcgagg ccgtccacgc
    10441 cgaatccagg aacgcctttc gccttcccgt tcgcccccct caaaaccccc agggcctgca
    10501 atgctccatt tgcctccatc aatgccgcat gggaccgggg gagaccgggt attgcggggt
    10561 tcgggacggc tcccggcaag cgtttcgcca caacgggcga acccgggccc tggtctccta
    10621 ctattacgat cctctgccca ccaactgcgt ggccgattgg gtttgcccgg ggggaaccgg
    10681 cgcggggtat ccccagtgtg ccaacgatcc gggtcccgag gtggggtttt acaacctggc
    10741 ggttttcttc gaggcctgca atttcaactg ccttttctgc cagaactgga ccttcaagaa
    10801 ggcctacgga tcccggccca actggcatca ggtccgggac ctcacccagg ccgtgaacgg
    10861 caaaaccagc tgcatctgct acttcggggg cgaccccacc acccaggtcc cctacgcgtt
    10921 acgcatgtcc gaagaggccc ggagggaaca tccccgccgg atcctgcgga tttgctggga
    10981 aaccaacggc agcgtccacc cggcttggct caaacgaatg gcccgacaat ccctggaaag
    11041 cggcggttgc gtcaaggtgg acctcaaggc ctgggattcg aggatccatc aggcactctg
    11101 cggctgcgac aaccgccggg tgatcgagaa cttcgcccgg ctcgcccgat ggattcccca
    11161 cagacccaat ccccccttcc tcattgccag cactcttctg gtgcccggct acgtggagga
    11221 ggacgaagtg agaggaatcg cctctttcat cgcgggactg gaccgggaca tcccgtacac
    11281 cctcctggct ttcgctcccc agtttcggat gaacgacttt cccaccacat cccgggatca
    11341 tgcggaatcc tgcctggaag cggcccgcag cgcaggcctc aggcgggtcc gcatcggaaa
    11401 cacccacctg ctgcgctgag cgaagaaggg ctctcaccgg gtc
//
LOCUS       DAOOVC010000124        11362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3709_length_11362_cov_6.741488, whole genome shotgun sequence.
ACCESSION   DAOOVC010000124 DAOOVC010000000
VERSION     DAOOVC010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11362
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_11362_cov_6.741488"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /gene="gltX"
                     /locus_tag="PLM79_13695"
     CDS             complement(<1..801)
                     /gene="gltX"
                     /locus_tag="PLM79_13695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOV87413.1"
                     /translation="MDAVITRFPPSPTGELHIGGARTALFNWLFARHYGGKFILRIED
                     TDQVRSTEASIRTILEAMEWLSLEWDEGPYFQTQRLDLYREYLARLLATGHAYYCECT
                     PQELEARRRAAMEAGKKPKYDGRCRDRGLGPGNNRVIRFRSPEVGTTVLNDLIKGPIF
                     FDNSELDDLVLQKSDGMATYNFAVVVDDITMNISHIIRGDDHVNNTPRQILIYQALGI
                     PLPQFAHVPMILGEDRTRLSKRHGATSVMAYKEMGFLPEALLNYLVRLG"
     gene            1057..1560
                     /locus_tag="PLM79_13700"
     CDS             1057..1560
                     /locus_tag="PLM79_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87414.1"
                     /translation="MRESVKWFALLLMLCAFWGCASNKGELISEPTAAVRSDVKIRVA
                     DVSNDTGKLFDVDVIGLLWNGLEDGLKKRGMLAAGPSGDAPLQIKAHVLKYKKGNAIG
                     RTLLPVGFSTVLYVRAELIQGATVLASAETKRDISFGEGAFARGMWKKVFEEVGEDLV
                     KQLTRKI"
     gene            1696..2256
                     /locus_tag="PLM79_13705"
     CDS             1696..2256
                     /locus_tag="PLM79_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOV87415.1"
                     /translation="MRVRVDEIPQGGRTLHFHWDDGQLKQHLPPRDPFDVTLAKPLNV
                     DLEIDKLTDHIRIEGKVRGTLGLTCHRCLKGLTQGLDEAVNVFLYEQQKAPAQEETQL
                     EADELNYEFFDGEVIDIDQLVLEQILLGLPVKVLCSEECLGLCPRCGANLNDESCGCA
                     KKPKDSPFAKLEAIRQELPNPEDLRK"
     gene            2403..2585
                     /gene="rpmF"
                     /locus_tag="PLM79_13710"
     CDS             2403..2585
                     /gene="rpmF"
                     /locus_tag="PLM79_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOV87416.1"
                     /translation="MGVPKRKTSKARRNKRRSHLHLSGPSLSRCPQCSEMRLPHRVCP
                     TCGYYHDRPVLEFEED"
     gene            2619..3686
                     /gene="plsX"
                     /locus_tag="PLM79_13715"
     CDS             2619..3686
                     /gene="plsX"
                     /locus_tag="PLM79_13715"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOV87417.1"
                     /translation="MRIAVDAMGGDHAPEALIEGVLLARGQGDAEILLYGDEGRTRKV
                     LEQLGGAVPPNVRIVHAPENIGMGEAGPMAIRKKREASLCMAMGALAAGTADAVVSAG
                     NSAAIVATAKHFVGLIPGLRRPAMVAPIPTPGGKVLLLDVGAHPEAHGIHLAQSAALA
                     QAYLKVTEGAPKTRVGLVNLGQEPVKGPKVVQRAHALLSRSRLNFVGNIEPHGLFGDQ
                     ADAVVCDGFTGNILLKMIEGLSEALLQLLEAQLDSRDGVTREKLSQTFSRFQRVHHYQ
                     NVGGAPLLGIQKPVVVAHGRSRGPAVAGAVVLASRLVQDGIFPRIAEELERDGVLAEL
                     KHHNAVLMLESLKTKWGFSPK"
     gene            3765..4934
                     /locus_tag="PLM79_13720"
     CDS             3765..4934
                     /locus_tag="PLM79_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOV87418.1"
                     /translation="MDYFLTEDQKMIQELAAQVAREKVRPVRAQLDEQEAFPWDVMKV
                     LAQSDLFGLYIPEEYGGLGGGIFENCLAVEKLAESCIGVATSFAASGLGAYPILLYGS
                     EDLKKKYLPLIASGQKLAAFALTESGAGSDVSGVKTTAVRDGEFYVLNGTKQWITNGG
                     EAEIYSVLAITDRTKGPRGASFFVVEKGDPGFSFGKKEKKLGIRASATRELVFQDCRI
                     PKDRIIGREGLGFIIAMKTFDKSRPGIGALGVGLAQGALDIAVEYARKRVQFGKPIIH
                     FQAIQHKLADMATKTEASRALIYCAARYMDTDPADSSKVAAMCKVFAGDVAMEVATQA
                     VQVLGGYGYMQDYPVEKMMRDAKILQIYEGTNEIQRNIIGQELNKEYGRLKESFF"
     gene            4947..5738
                     /locus_tag="PLM79_13725"
     CDS             4947..5738
                     /locus_tag="PLM79_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV87419.1"
                     /translation="MHLVVCIKQVPDTKNVRIDPDTHTLVRQGVESIVNPFDLFALEA
                     ALQIKDRLNARITVLTMGPPQAEEALRDTLSRGADEAVLLSDRAFAGSDTWATSTTLS
                     AAIRKMGNVDLVLCGKQAVDGDTAQVGPEVATLLDIPYATFVKKIEVLDDRALKVTRQ
                     TDEGMEIWRLPTPALLTVIKEVGEPRLPSLRHKMRARKAQIPVWGVGDLGLDPGRVGL
                     SGSFTQVVRVFSPPRRGNRVLLHGSPEEQAEQLYRNLKEAKVPGI"
     gene            5735..6934
                     /locus_tag="PLM79_13730"
     CDS             5735..6934
                     /locus_tag="PLM79_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOV87420.1"
                     /translation="MKAVVDPKKCTVCGVCAEACPFGAITVGETSVDVSDECTLCGMC
                     VDTCEFGAISLPEAGAGVSTDSAEYSGIWVYAEWRMGTIHKVSHELLSAGRKLADRRG
                     VALCAVVLGHLLDEDLLRELPEYGADRVYVVDHPRLAHFTDESYANALVELVRLKKPE
                     ILLAGATSMGRSFIPRVAALLGSGLTADCTDLDISDEGLLLQTRPAFGGNVMATIVCP
                     HRRPQMATVRPKVMRPRKIEGRSGEIERLSVPEECLVGRVEVLEVIPERDQTAKLTEA
                     DVIVSGGRGLRKPENFALLEDLAHLVNGAVGASRGAVEEGWIAPSHQVGQTGRTVAPT
                     LYMAVGLSGAIQHVVGMQGSKVIVAVNKDPDAPIFEVANYGIVADLFEFVPALIRRIR
                     QERGEDS"
     gene            6955..7698
                     /gene="fabG"
                     /locus_tag="PLM79_13735"
     CDS             6955..7698
                     /gene="fabG"
                     /locus_tag="PLM79_13735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOV87421.1"
                     /translation="MSDKPVIVVTGASRGIGRAVAVELTEPGAVVVVNYHSKTAEAQE
                     TAAAVEAKGGVPRLYRFGVGDEEAVKEAFKQILQEFKKVDVLVNNAGVVRDNLLALMK
                     VAEWDEVIRTNLRGAFLCSQAVVKPMMRRRYGRIVNVASVVGVTGNAGQCNYSASKAG
                     IIGLTRALAHELISRNITVNAVAPGFIETDMTMRIPEKFREAILDQIPAGRYGAPEEV
                     AAAVKFLASKGAGYITGQVLHVNGGLYMG"
     gene            7771..9045
                     /gene="fabF"
                     /locus_tag="PLM79_13740"
     CDS             7771..9045
                     /gene="fabF"
                     /locus_tag="PLM79_13740"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOV87422.1"
                     /translation="MEKKGMGFTERRRVVVTGIGLVSPLGVGVEENWDALIRGQSGIG
                     PITHFDAGAFATRIAGEVKNYRPEDFIPKKELRKLDPFLKLALGAARLGLEDARLTIP
                     DQLAPRAGVIMGCGLGGLTTIEETHKVLLDAGPKKVSPFFIPMLIGNMAAGLISIYHN
                     AKGPNLSIQTACAAGTHAVGQAFHMIRNGMADIMITGGAESTITPLCVAGFNSMKALS
                     TRNDDPEKASRPFDRDRDGFVLGEGGAVLVLEELGLALERGARIYAEMLGFGTSGDAF
                     HMAAPAPDGEGAARCMQLALEDAGIAPVEVDYINAHGTSTDLNDKYETQAIKKIFGEH
                     AKRLAISSTKSMTGHLLGAAGGIEAAYTALTIARDLIPPTINYENPDPDCDLDYVPNR
                     AREAPVGKAMSNSFGFGGTNGTLVLGKWNGGA"
     gene            9115..9564
                     /gene="rpiB"
                     /locus_tag="PLM79_13745"
     CDS             9115..9564
                     /gene="rpiB"
                     /locus_tag="PLM79_13745"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOV87423.1"
                     /translation="MKIAIACDHGGFELKETVTAHLKSRGCGVTDLGTHGTDSVDYPQ
                     YAMAVVREVLEERADRGILICGSGIGMCMTANRVPGVRAIMASEPYAAKMGRRHNNSN
                     ILCLGGRFIGPGLALEIVDTWLQEPFEGGRHQRRLDLMEELARGFAS"
     gene            9708..10955
                     /gene="glyA"
                     /locus_tag="PLM79_13750"
     CDS             9708..10955
                     /gene="glyA"
                     /locus_tag="PLM79_13750"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOV87424.1"
                     /translation="MSYLDVIDPEITQVLLEEEQRQRGKLELIASENFVSDAVREVQG
                     SVLTNKYAEGYPGKRYYGGCEFVDVAEKLAQQRAKKLFGADYVNVQPHSGSQANMAVY
                     FSVLQPGDTILGMDLRQGGHLTHGSPVSFSGKLFRVVSYGVRRDTETIDYEQLEELAR
                     EHRPRLIIAGASAYPRIIDFSRFRAVAESVGAFLMVDMAHIAGLVCTGLHPNPVPYAD
                     FVTSTTHKTLRGPRGGLILSHREHGKSLDSQIFPGIQGGPLMHVIAAKAVAFQEALQP
                     SFREYQKRVVENAGRLASELTSLGYRLVSGGTDNHLMLIDVAPRGLTGKMAEETLDKA
                     GITVNKNSIPFDPQKPGITSGIRIGTPAVTTRGMTPDHMILVAHFIHRALQAADKELT
                     LQNLRTEVAEFCSAFPLHRADPV"
     gene            11040..>11362
                     /locus_tag="PLM79_13755"
     CDS             11040..>11362
                     /locus_tag="PLM79_13755"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOV87425.1"
                     /translation="MKPTTPHDVYMRQALALAARGRGTTSPNPMVGAVVADRTGVVGK
                     GYHAFPGGPHGEVNALRDAGDRAVGATLYVTLEPCNHFGRTPPCTGAILDAGISRVVV
                     GMRDP"
BASE COUNT         2322 a         3340 c         3656 g         2044 t
ORIGIN      
        1 gcccaggcga accaggtagt tgagcagggc ctcgggaagg aaccccatct ccttgtaggc
       61 catgacggat gtggccccgt gtcgcttgga gagtcgagtc cgatcttcgc ccaggatcat
      121 gggaacgtgg gcaaattggg gcagaggaat gcccagggcc tggtagatga ggatctgccg
      181 gggggtgttg ttcacatggt cgtctccccg gatgatgtgg gagatgttca tggtgatgtc
      241 gtccacgact acggcaaaat tgtatgtggc catcccgtcg cttttctgga gcacgagatc
      301 gtccagttcc gagttatcga agaagatcgg acccttgatc aagtcgttca ggacggtggt
      361 gccgacctcc ggactgcgaa accggatcac ccggttgttc cccggtccca atccccggtc
      421 ccggcatcgt ccgtcgtact tgggcttctt cccggcttcc atggccgccc gccttcgggc
      481 ttccagttcc tggggagtgc attcgcagta gtaggcgtgc cccgtagcca ggagccgggc
      541 caggtattcc cggtagaggt ccaatcgctg ggtttgaaag taaggtcctt cgtcccattc
      601 cagggagagc cattccatgg cctccagaat ggtccggatg gaagcttcgg tagaccgcac
      661 ctggtccgtg tcttcgatgc ggagaatgaa ctttcccccg tagtgtcgtg caaacagcca
      721 gttgaaaagg gcggttctcg cccctccaat gtgaagctcc cccgtcgggc tgggaggaaa
      781 tcgcgtgatc actgcatcca tggaaatcgt gctccttcag aaatgctcca tccgatcgtt
      841 tgcaaaggac tgggccgaac aaatcgccga aaaggtaacg cattcgtgtt cttatgaaaa
      901 ggaaaacctg tacggcaccg gttggcaagg gcacagagaa aacttgccca ttggagtctc
      961 atctggcagt atgggctggc aagaaaaaac ggagggacgc aacgggatca agggcttttc
     1021 atcgcctcgg cggcaagggt ggaagggaac aggatcatga gagaatccgt caagtggttc
     1081 gctttgcttc tgatgttgtg cgcgttttgg ggttgtgcgt ccaacaaggg ggaactgatt
     1141 tcggagccga cggcggccgt tcgctcggat gtgaagattc gcgtggccga cgtgtccaac
     1201 gacacgggaa agctgttcga cgtggacgtg atcgggttgc tttggaacgg cctggaagac
     1261 ggattgaaga agcggggaat gctggctgcg ggaccttctg gggacgcccc cctgcaaatc
     1321 aaggcccatg tgctcaagta caagaagggg aacgccattg ggcggactct tctccccgtg
     1381 gggttcagta ccgtcttgta tgtgcgggcg gaattgatcc agggcgcaac ggtccttgcc
     1441 tcggcggaga ccaagaggga catttctttc ggcgaagggg cgtttgcccg gggcatgtgg
     1501 aagaaggttt ttgaggaagt gggcgaagac ctcgtgaagc agttgacacg gaagatataa
     1561 agggggaacg gggaagaaga acggcgggga cggggattgg tctttgacct catcccgggg
     1621 aaggattaaa attccttgac acgctgaaga ggatttctat actcacccat ttccacaggt
     1681 aggcgagagg gaaccatgag ggttcgcgtg gatgaaatcc cccaaggggg aaggaccctt
     1741 cactttcatt gggatgacgg acagctcaaa caacatctgc cgccccggga tccctttgac
     1801 gtgacgctgg caaagccgct caacgtggac ctcgagatcg acaagttgac cgatcacatc
     1861 cggatcgagg gaaaggttcg agggaccctt gggctcacct gccatcgctg cctcaagggc
     1921 cttacccaag gcctggacga agcggtgaac gtgtttctct acgagcagca aaaggccccg
     1981 gcccaggaag aaacccagct ggaggccgat gagttgaact atgaattctt cgatggcgaa
     2041 gtcatcgata tcgaccaact ggtcctggaa cagatccttc tcggtctccc cgtcaaggtg
     2101 ttgtgctcgg aagaatgcct cggtctctgt ccccgctgtg gtgcaaacct caacgatgaa
     2161 tcctgcgggt gtgcaaagaa gcccaaggat tcgccgtttg ccaagctgga agccatcagg
     2221 caagagcttc ccaatcctga ggacctccga aaataacgtc cccttggaat ccggcacagg
     2281 acggagactt gactgccctt cccggggcgg tcggcaggac ggccttttgc ggggaagctc
     2341 ccggggccgg gcggtcgcga ccgtttttgg ggaattttga aaccagaaga ggagaatgaa
     2401 ccatgggggt acccaaacga aaaacgtcca aggcccgacg caacaagcga cggtctcacc
     2461 tgcacctctc cggaccctcc ctgtcccgct gcccccagtg cagtgaaatg aggctgcccc
     2521 atcgagtctg tccaacgtgc ggctattatc atgaccgtcc cgtgttggaa ttcgaagaag
     2581 attagtcccg cgagccggac cgcaaggggt ctttgcccat gaggattgcc gtagacgcca
     2641 tggggggaga ccacgccccg gaggcgctca tcgaaggggt cctcctggca agagggcaag
     2701 gggacgcgga gattctactt tacggagatg aggggaggac gcggaaggtc ctcgaacaat
     2761 tgggaggggc ggttcctccg aacgtgcgca tcgtccatgc acccgagaac atcggcatgg
     2821 gagaagccgg tcccatggcc atccggaaaa agagggaagc ctctttgtgc atggccatgg
     2881 gggccctggc cgccgggacc gcggatgccg tggtgagcgc gggcaacagt gccgccattg
     2941 tggccacggc caagcatttc gtgggcctga tcccgggatt gagacgcccc gccatggtcg
     3001 ctcccatccc cacccccggg gggaaagtgc tcctgctgga tgtaggtgcc catcccgagg
     3061 cccacgggat tcacctcgct caatccgccg cattggccca agcgtatctc aaggttaccg
     3121 agggggcccc aaagacccgg gtaggactgg tcaacctggg gcaggaaccc gtcaagggac
     3181 ccaaggtggt ccagcgagcc catgccctgc tcagcaggtc ccgcctcaac ttcgtgggaa
     3241 acatcgagcc ccacggtctt tttggagacc aggcggacgc cgttgtctgc gacggtttta
     3301 ccgggaacat cttgctgaaa atgatcgaag gactctccga agccctgctc cagttgctcg
     3361 aagctcaatt ggattccagg gatggggtca cccgggaaaa gctgagccag acgttcagtc
     3421 ggtttcaacg ggttcatcac tatcagaatg tggggggggc ccctttactg gggatccaaa
     3481 aaccggtggt cgtggcccac ggacgctccc gggggcctgc agttgccggt gccgtggtct
     3541 tggcttcccg gttggtccaa gacggaattt ttccccggat tgccgaggaa ctggagaggg
     3601 atggggttct ggcggaactg aaacatcaca atgccgtgtt gatgttggag agcctcaaaa
     3661 ccaagtgggg attcagcccg aagtagcctt gccgccccgg tcccccgtca ccgtcccccc
     3721 ccttatgccg gggggaagga attttcccaa aaggagaaag cacggtggat tatttcttga
     3781 ccgaagacca aaaaatgatt caggagctgg ctgcccaggt ggcacgggag aaggtccggc
     3841 ccgtgcgggc ccagttggac gaacaggagg ccttcccctg ggacgtcatg aaggtgctcg
     3901 cccaatcgga cctgttcgga ctttacatcc ccgaggaata cgggggattg ggagggggaa
     3961 tcttcgagaa ctgcctggcc gtggaaaaac tggccgagtc ctgcatcggg gtggccacct
     4021 cttttgcggc cagcggcctg ggagcctatc ccatcctcct ttacgggagc gaggacttga
     4081 agaagaaata ccttcccctc attgccagcg gccaaaaatt ggctgccttc gccctcaccg
     4141 agtccggtgc gggcagcgac gtttccgggg tgaagaccac cgccgtccgg gacggggaat
     4201 tctacgtcct taacggaacc aagcagtgga tcaccaacgg gggggaggcg gagatctatt
     4261 cggtcttggc catcaccgac cggaccaaag ggccccgggg ggccagcttc tttgtggtgg
     4321 aaaaagggga cccggggttt tccttcggga agaaggaaaa gaagctgggc atccgggctt
     4381 cggccacccg ggagctggtt ttccaggact gccggattcc caaggaccgc atcatcggcc
     4441 gggagggcct gggattcatc attgccatga agaccttcga caagtcccgc ccgggaatcg
     4501 gggcgctggg cgtcggcctg gcccagggag ccctggacat cgccgtggaa tatgcccgca
     4561 agcgggtcca gttcggcaag cccatcatcc acttccaggc cattcagcac aaactggcgg
     4621 acatggcgac caagaccgaa gcctcccgcg ctctgatcta ctgtgcggcc cgctacatgg
     4681 acacggaccc tgccgattcc agtaaggtgg cggccatgtg taaggtcttc gccggggatg
     4741 tggccatgga agtggccacc caggcagtcc aggtcctggg gggatacggc tacatgcagg
     4801 actaccccgt ggagaaaatg atgcgggatg ccaagatcct ccagatctac gaagggacca
     4861 acgaaatcca gcgaaacatc atcggccagg aactcaacaa ggagtacggc cgactcaagg
     4921 agtccttttt ctgagaggag accgtcatgc acctcgtggt ttgcatcaaa caggtgcccg
     4981 acaccaagaa cgtccgcatt gatcccgaca cccataccct ggtgcggcag ggggtggaat
     5041 ccatcgtcaa tcccttcgat cttttcgcct tggaagcggc cctgcagatc aaagaccggc
     5101 tcaatgcccg cattaccgtc ctcaccatgg ggccgcccca ggccgaggaa gccctccggg
     5161 acaccctctc ccgcggcgcg gacgaggcgg tgctcctcag cgaccgagcc ttcgcgggct
     5221 ccgatacctg ggccacttcc accaccctct ccgccgccat tcgcaagatg gggaacgtgg
     5281 atctcgtcct ttgcggaaaa caggccgtgg acggggacac cgcccaggtg ggccccgagg
     5341 tggccaccct cctggacatt ccctacgcca ctttcgtgaa aaagatcgaa gtgttggatg
     5401 atcgcgccct caaggtgacc cgccagacgg atgaaggaat ggaaatctgg cggctcccga
     5461 ccccggccct cctgaccgtg atcaaggaag tgggggagcc gcgcctgccc tccctgcgcc
     5521 acaagatgag ggccaggaag gcgcagattc ccgtgtgggg agtcggggac ctggggctgg
     5581 atcccggccg ggtggggctt tccgggtctt ttacccaagt ggtgcgggtc ttcagtcccc
     5641 ctcgccgagg aaaccgggtc ttgctccacg gctcccccga agaacaggcg gaacagcttt
     5701 accgtaacct caaagaagcc aaggtgccgg gaatatgaaa gccgttgtcg atccgaagaa
     5761 gtgcaccgtc tgcggcgttt gcgccgaagc ctgccctttt ggcgccatca ccgtcgggga
     5821 gacttccgtg gacgtttccg acgagtgcac gttgtgcggc atgtgcgtgg atacctgtga
     5881 attcggggcc atttccctgc ccgaggcggg ggcgggggtc tcgacggact cggccgaata
     5941 ctcggggata tgggtttatg ccgagtggcg catgggaacg atccacaagg tgagccatga
     6001 gctcttgagc gccgggagga aactggcgga ccgaagaggg gtcgctctct gtgccgtggt
     6061 cctggggcat cttttggacg aagacctgct gagggagctc ccggaatacg gggcggaccg
     6121 ggtctacgtc gtggaccatc cccgattggc ccatttcacg gacgaatcct acgccaacgc
     6181 cctggtggaa ctggtgcgcc tcaagaaacc ggagatcctc ctggccgggg ccaccagcat
     6241 gggcaggtcc ttcattcctc gagtggcggc cctcctgggc agcgggctca cggcggactg
     6301 cacggacctg gacatcagcg acgaggggct cctgctccag acccgccctg ccttcggcgg
     6361 caacgtcatg gccaccatcg tgtgccccca tcgccgcccc caaatggcca cggtacggcc
     6421 caaggtcatg cggccccgga agatcgaagg ccgctcggga gaaatcgaga ggctttccgt
     6481 tcccgaggag tgtcttgtcg ggcgggtgga ggtgctcgag gtgatcccgg aaagggacca
     6541 gacggccaag ctcaccgaag ccgacgtgat cgtttccggg ggtcgaggat tgcgcaagcc
     6601 cgaaaacttt gccctcctcg aagacctcgc ccacctcgtc aacggggccg tgggggcttc
     6661 tcgaggggcc gtcgaggaag gctggatcgc tccttcccac caggtgggcc aaacgggacg
     6721 cacggtcgct cccaccctct acatggccgt gggcctgtcc ggcgccattc agcacgtggt
     6781 gggaatgcag ggctccaagg tcatcgtcgc cgtgaacaag gaccccgacg ctcccatctt
     6841 tgaagtggcc aattacggga tcgtggcgga cctgttcgaa ttcgtcccgg ccctgattcg
     6901 aagaattcgg caggaacgcg gggaagattc ctgaaattgc caggaggggg aaccatgagt
     6961 gacaagccgg tgatcgtggt cacgggagcc agccggggga tcgggcgggc cgttgccgtc
     7021 gagttgaccg agcccggggc ggtggtggtg gtgaactacc attccaagac ggcggaagcc
     7081 caggaaacgg cggccgccgt ggaagccaag gggggagttc cccgcctgta ccgattcggc
     7141 gtaggggatg aagaggccgt caaggaggct ttcaagcaga ttctccagga gttcaagaag
     7201 gtggatgttc tggtgaacaa cgcgggagtc gtccgggaca acctgctggc cctcatgaag
     7261 gtcgccgaat gggacgaggt gatccggacc aatctccggg gcgccttcct gtgttcccag
     7321 gccgtggtga aacccatgat gcgccggagg tacggacgga tcgtcaatgt agcctcggtg
     7381 gtcggggtca cgggaaacgc cggacagtgc aactattcgg cctccaaggc gggaatcatc
     7441 gggctgaccc gtgccctggc ccatgagctc atttcccgga acatcaccgt caacgcggtg
     7501 gctcccgggt tcatcgaaac cgacatgacc atgagaattc ccgaaaagtt ccgggaggct
     7561 atcctggacc aaatccccgc gggcaggtac ggggcccccg aggaagtggc cgccgccgta
     7621 aaattcctgg cctccaaggg ggcggggtac atcacgggcc aggtcctcca cgtcaacggc
     7681 ggcttgtata tgggctgacc tgccggttcg ggttgaaagg cttgcttttc attgcgttgt
     7741 ggggtcaacg gctgcaattg gacaaggtgc atggagaaaa aaggtatggg gtttacggag
     7801 cgtcgtcgag tggtggtcac gggcatcgga ctggtatccc ccctcggggt tggcgtggag
     7861 gagaattggg atgcactgat ccggggacaa tccgggatcg gacccatcac ccacttcgat
     7921 gcaggggcgt ttgccacccg catcgccggt gaggtcaaga actatcgtcc cgaggatttc
     7981 attcccaaaa aagagctccg caagctggac ccgtttctca agctggcctt gggggccgct
     8041 cgactgggac tcgaggacgc ccggctgacc atccccgacc agctggctcc cagggccggg
     8101 gtgatcatgg ggtgcggact ggggggcctc accaccatcg aggaaaccca caaggtccta
     8161 ctggatgcgg gccccaaaaa agtcagccct tttttcatcc ccatgctcat cggcaacatg
     8221 gccgccgggc tcatctccat ttaccacaac gccaaggggc cgaacctttc catccagacg
     8281 gcctgcgcgg ccgggactca cgccgtggga caggccttcc acatgattcg caacggaatg
     8341 gcggacatca tgatcaccgg gggggccgaa tccaccatca cccccttgtg cgtggcggga
     8401 ttcaactcca tgaaagccct ctccacgaga aacgacgatc ccgaaaaggc ttcccgcccc
     8461 ttcgatcggg accgggacgg gtttgtgctg ggagaagggg gtgcggtgct ggtgctcgaa
     8521 gagctgggac tggccctgga gagaggggcc cgaatctatg cggaaatgct gggattcggg
     8581 acctccgggg acgcctttca catggcggcc cccgcccccg acggagaagg ggcggcccgt
     8641 tgcatgcagt tggccctcga ggatgccgga atcgcccccg tcgaggtgga ctacatcaat
     8701 gcccacggca cgtccacgga cctgaacgac aagtacgaaa cccaagccat caagaagatt
     8761 ttcggggaac acgccaaaag attggccatc agttccacca agtccatgac gggtcacctg
     8821 ctgggtgccg cgggggggat cgaagcggcc tatacggcgc tcaccatcgc ccgggacctc
     8881 attccgccca ccatcaacta cgaaaatccc gatcccgatt gcgacctgga ctacgttccc
     8941 aaccgggctc gagaagcccc ggtgggcaag gccatgtcca attccttcgg ctttggggga
     9001 accaatggaa ccctggtcct ggggaaatgg aacggagggg cgtgatctcg tcgggaatcc
     9061 ttgatcccgg tacaatgata ttgcatactt aaagtccaaa acaggcagtt cgagatgaag
     9121 atcgccatcg catgtgatca cggtggattc gagctcaagg aaaccgtaac ggctcacttg
     9181 aaatcccggg gctgcggggt gacggacctg gggacccacg ggacggattc cgtggactac
     9241 ccgcagtacg cgatggcggt ggtccgggag gtcctcgagg aaagggcgga tcggggcatt
     9301 ctcatttgcg gaagcggcat cggcatgtgc atgacggcca accgcgttcc cggagtgcgg
     9361 gcgatcatgg cttcggaacc ctatgccgcc aagatgggcc gacgccacaa caacagcaac
     9421 atcctgtgcc tgggcggccg attcatcggt cccggcctgg ccctggaaat cgtggatacg
     9481 tggctgcagg agcccttcga gggcggacgc caccagaggc gcctggactt gatggaggag
     9541 ttggcccggg gattcgcgtc ctaggcggtg ggaagcggca ccgaacccca tcgggagtcc
     9601 cgaggtccag gggggcgggg attcggcgtc gggggcgacc accgaggatg ccgtgggagg
     9661 gttttcggga atgaggcccg tcccgttttg tacacaagga aagcaccatg tcctaccttg
     9721 acgtcatcga tcctgagatc actcaagttc tactggaaga ggaacagcgc cagaggggaa
     9781 agctggaact gatcgcgtcg gagaatttcg tcagcgacgc cgttcgagag gttcaaggtt
     9841 ccgtgctgac caacaagtac gccgaagggt accccggcaa gaggtactac ggaggatgcg
     9901 agttcgtgga cgttgccgag aagctggccc aacagcgggc caagaagctc ttcggggcgg
     9961 attacgtcaa cgttcaacct cactcagggt cccaggccaa catggccgta tatttctcgg
    10021 tgcttcaacc cggcgatacc atcctgggca tggacctgcg ccaggggggg catcttaccc
    10081 acggaagccc cgtgagcttt tcgggcaagc tcttccgggt ggtgtcctac ggagttcggc
    10141 gggatacgga aaccatcgac tacgagcagt tggaggaatt ggcccgggag catcgtcctc
    10201 ggctgatcat cgcgggggca agcgcctatc cgcgcatcat cgatttttcc cggttccggg
    10261 ccgtcgccga gtccgtgggg gcgtttctca tggtggacat ggcccacatc gccgggctgg
    10321 tctgtacggg actgcatccc aatcccgtcc cctatgccga ttttgtgacc tccaccaccc
    10381 acaagaccct gcggggaccc cgggggggac tgattctctc ccaccgggaa cacgggaaaa
    10441 gcctcgacag ccagatcttt ccgggaatcc agggggggcc gctcatgcac gtcatcgccg
    10501 ccaaggccgt ggccttccag gaagccctgc agccgtcctt ccgggaatac cagaaacggg
    10561 tggtggaaaa cgccgggagg ctggcctcgg agctcaccag cctgggctac cggctggtat
    10621 ccgggggcac cgacaaccac ctgatgctca tcgatgttgc cccccgggga ctcacgggaa
    10681 agatggccga agaaaccctg gacaaggccg gaatcacggt gaacaagaat tccatcccgt
    10741 ttgatcccca gaagcccggc atcaccagcg ggatccgcat cgggaccccc gcggtgacca
    10801 cccgagggat gacccccgac catatgatcc tggtggccca tttcatccac cgagcgctcc
    10861 aggccgcgga caaggagctt accctgcaga acctgagaac ggaggtggcg gaattctgct
    10921 ccgccttccc ccttcaccgg gcggatcccg tctgaagaga gaaggggaac ccgagagcat
    10981 gtcccgcccc ggggagaaaa gacgccggac ctcaacccgg cgctcttttt tggatgccca
    11041 tgaagcccac aaccccccat gatgtctaca tgagacaggc cctggccctg gccgcccggg
    11101 gcaggggaac caccagcccg aaccccatgg tgggagcggt ggtggcggac cggaccgggg
    11161 tcgtgggaaa gggataccat gcctttcccg ggggacctca cggggaagtc aacgccctga
    11221 gggatgccgg ggaccgggcc gtcggggcca ccctctacgt gaccctggaa ccctgcaacc
    11281 atttcggacg gacccctccc tgcaccggcg ccattctcga tgcggggatt tcccgggtgg
    11341 tcgtggggat gcgggacccg aa
//
LOCUS       DAOOVC010000125        11168 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3789_length_11168_cov_6.184649, whole genome shotgun sequence.
ACCESSION   DAOOVC010000125 DAOOVC010000000
VERSION     DAOOVC010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11168
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3789_length_11168_cov_6.184649"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            345..1535
                     /locus_tag="PLM79_13760"
     CDS             345..1535
                     /locus_tag="PLM79_13760"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HOV87426.1"
                     /translation="MSPGKALGQRLITKEERVNTYELDDHLILQPDSSNRFLVLNETA
                     GIVWKAHRRGCSLTEICEELSCRFGISRKQAAGDVATVFRTMESAGTIDSEAEVNEKM
                     SFVSAPDRSTSCPAESEKDLRVAYSLWGRPFVVAYGSRRLQDMLHSYLSHLVCKEFAD
                     APLRYALMESEKTYFVLKNGDLLSRTTDAYKVKNTLMLDILAHCHEKERFGAVWHAAA
                     ITCKHACILLPAASGSGKSTLTAALIREGYGYMGDDFIVLSASGFDALAAPFALSIKE
                     TSWPVLKSLYPEIDDLPVYSPSHKTVKFLHPPRESFGLAFRRMPVHCIIHPRYVPGSS
                     ESMEAISPVVSFQSLIAANAWLDTDASNIAGFLRWLERRKSYLMTYSSISGARRLLEA
                     VLTV"
     gene            1532..2707
                     /locus_tag="PLM79_13765"
     CDS             1532..2707
                     /locus_tag="PLM79_13765"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOV87427.1"
                     /translation="MNRIQAAKFLGRLITGPESPEDREQVNRRLRGAMPWELLVDVAG
                     GNLITPALHTALSSLDEYKNLPEDLRTYLAYIHRLNRSRDEDVQSQVCEISRLFNQIG
                     VEPVLLKGAANLFLDIYRDPADRIMHDVDVLVPKEKLSECTNILKENGYRGILKNFSF
                     TEESPHFFILVSRERPVGVELHQYTLPKQYATIYTDRDLARESVRQSVADAVFRLPSP
                     PYHLLHHVCHAHKHWKVYGGPAQNSLRFAVDFTLLCRFYGDQIDWIGIEKRFEAGKLR
                     NALAAYLLATKELCGFGDPLARVGVSRAKERFLRGVFFLFRFHPVFGACKLFHEYRLS
                     IKAMAADPAERIDFLKKLNRKEFYARQGQRLMGIFKKEHALKNLCDMDREGAEYGRD"
     gene            2694..2876
                     /locus_tag="PLM79_13770"
     CDS             2694..2876
                     /locus_tag="PLM79_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87428.1"
                     /translation="MVETNHPNPENQAVADSEKPSEELITRKEAFKKMGKYAAYSAAV
                     VAVLLSSPDKAVASPL"
     gene            complement(3174..4013)
                     /locus_tag="PLM79_13775"
     CDS             complement(3174..4013)
                     /locus_tag="PLM79_13775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87429.1"
                     /translation="MSGREHGRRAGDQVLRVLAGESADQVPVDLSITARPMFDYVQLQ
                     RFQIPLTALPADSVVLNKPDSLWESHRILVLATLAALTILTALVLLLTFSVLRRRRAE
                     QALSESETRYRILFQHANDAVFLADCGTHRIVDCNHKALELAGYTTEELRHMSVEELH
                     PPEERTRVREIFERAAAGESCNEVSDLHCLRKDGGLVDIECNSVRLELGERAHQLIVV
                     RNIAEKRRVEAEKAGLERQLVQAQNVIAHRGVLKEGIHFIQKPFTMRDVARKIREILG
                     TAE"
     gene            complement(4010..4954)
                     /locus_tag="PLM79_13780"
     CDS             complement(4010..4954)
                     /locus_tag="PLM79_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87430.1"
                     /translation="MKTPVVRPKFVAGNIQHRSGDELALDAGVRKIGLRILIRRSLVF
                     RTTCLVALCLVAAAHLLFPALSAAAREVPRLVILNSYHPGFAWSDGEMQGLVERIHEV
                     HPGIELPIEFLDAKRRPGRKHMVRVKDFLLEKYRGRKIDVVVCLDNPALTMALRFRDE
                     LFPGVPLVFAGVSDFSLELANGHRNVTGVAERMDVGGTLEASLALHPRTTRVYVVNDE
                     TLSGRAARREAEPVARSLPGRVEVRFLPAGTFDELVAEMGSLPPDSLASILSFATDTV
                     VESRSLADSTRLLTSAARVPSTAFTKRGWVMELSAAGS"
     gene            5275..6126
                     /locus_tag="PLM79_13785"
     CDS             5275..6126
                     /locus_tag="PLM79_13785"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOV87431.1"
                     /translation="MDRNDPVGAAAEALREAEAVLVTAGAGMGVDSGLPDFRGNHGFW
                     KAYPAIARLGKSFVEMANPEWFHRDPRLAWAFYGHRLNLYRRTVPHEGFRLLLEVARK
                     KPRGYFVFTSNVDGHFQKAGYDESRIEECHGSIHHLQCCEPCSDDIWDGTGLEIDVDE
                     DAFEAHEPLPRCRRCGSMARPNILMFGDWSWVSGRTEDQGRRLSEWVRSVERSRCRAA
                     VVELGAGTAVATVRRLSEQLAHRLGSPLIRINPRDFGSSFSGNIPIPVGAAEGIRAVV
                     SRMGGTA"
     gene            6144..6605
                     /gene="sixA"
                     /locus_tag="PLM79_13790"
     CDS             6144..6605
                     /gene="sixA"
                     /locus_tag="PLM79_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HOV87432.1"
                     /translation="MALYLVQHGKAVPREQDPEQGLSREGIRDVERIGMVAKGYGVRV
                     ASIQHSGKRRARQTAEILASTLDSVQEVREIPGIGPLDDATLLAPSLKSRDNRMLVGH
                     LPFLEKLTSTLLTGSADLRIFKFQNGGIVALDQDPESGSWYIKWTLMPHIS"
     gene            6723..8240
                     /locus_tag="PLM79_13795"
     CDS             6723..8240
                     /locus_tag="PLM79_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HOV87433.1"
                     /translation="MLALFLGALDALVVAAAMPSIVADLGGIHLYSWVFSSYMLTRAT
                     ALPIFGKLCDLYGSRNLFLAAVALFMASSVLAGAADSMPYLIFFRALQGIGAGGNFAL
                     AYVIAADLAPPHKRGKAMGSMAFVWGVASILGPSLGGFMVNYFSWRWIFYINLPLGAF
                     AFLSIALFLKEVRQKKPNASVDYPGALVLTLSVLALLTAFLLGEHWDQWLSPTIPALL
                     VASAAGVFLFARIEKRAPEPILAMEFFRIPAFRTANGAAFCSSFAIFSLSAFTPLYIQ
                     GVLGKSPATLGLAMIPLSLGWSLGALVCGQVIRPHRERRFSIWGALVLAGSTAMILPF
                     TPGTPLWLCSAVLAAAGLGMGFVSISTLLVVQNSLSASDLGIATSSHQFARTLGGTIG
                     IGASGSFFSGALTRWTDHPANARWARDFPQFFGQDLPGNLRHLFQSDLSGMLPQSLQN
                     VLREAVLGGIDIVVGLALAASVLNVLCCFLLPKEGPRSPDAAPPPVNFPVRGGMK"
     gene            8237..9466
                     /locus_tag="PLM79_13800"
     CDS             8237..9466
                     /locus_tag="PLM79_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOV87434.1"
                     /translation="MMEELEKNEPQEEESFEELLKKSRVVSTPLRRGEKVKTVVLKIT
                     PEWTFLDLGGKSEGLLSTKEIQDEEGNPTVKEGDVLEAFFLSSEGNETRFTTRITGKE
                     AAAAYLEDALAGGIPVEGVIEKEIKGGYQVKLPGNVRGFCPFSQMGLPRTRGNLDLTG
                     QRMTFKVIEYEREGRRVLLSNRAILEEARRVQVNALKESLKEGMIVRGTVVSVKEFGA
                     FLDIGAVQGLIPISEIGYGRVEDIHQWLKVGQEVEVMVVKLDWEKERFSFSIKAVLPD
                     PWEDVPAKYPEGSVHRGTVTRPMEYGAFVQLEPGVEGLLPISEFRTGRKVKHARQLVT
                     EGQSLTVKVLSVDGEARRISLGIQWESSEEGDAPVESAAAYAGSGSKSMGTLGDLLTA
                     KGAGKERREPGKGKRKR"
     gene            complement(9507..9884)
                     /locus_tag="PLM79_13805"
     CDS             complement(9507..9884)
                     /locus_tag="PLM79_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV87435.1"
                     /translation="MALNVTSEEKQPGIFTVLPFGRLDSETYWVLEKRIDFMLNEGRA
                     RVVTLDMKGVDYISSMGIRVILKARKGLAQRNGNLLIVNLQPQIKKVFEIINALPTLQ
                     IFASIEELDAYLTEMQRRALEGE"
     gene            complement(9924..10334)
                     /locus_tag="PLM79_13810"
     CDS             complement(9924..10334)
                     /locus_tag="PLM79_13810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87436.1"
                     /translation="MKTIQLSATMESLEPLEAFVLEPARALGAPAEMLGDMKLVLEEV
                     LVNIISYGYPGSRGDIRVDWTADENGTCRIRIRDWGVPFNPLEAPAPDLDVDFTERTV
                     GGLGILLVRQLAHDVWYERDDDSNVLTLCFQLRS"
     gene            complement(10476..>11168)
                     /locus_tag="PLM79_13815"
     CDS             complement(10476..>11168)
                     /locus_tag="PLM79_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOV87437.1"
                     /translation="REIQDVLLSGGDPLLLAPSKLDHILSSLRRISHVGIVRMGTRVP
                     GFSPATVTLELARMLSRHHPLFMNLHFNHPRELTQEAAKACGILADAGIPLGSQTVLL
                     RGVNDDPSVLGELFQGLLRLRVRPYYLMQMDLTRGTAHFRTPLARGLEVLSRLRNRIS
                     GLAMPHLVVDLPGGRGKVPLTPNRVEAREEDRLVFLDYRGERVSYPLLPGEGDELNKW
                     LDSPLRVARESI"
BASE COUNT         2185 a         3369 c         3410 g         2204 t
ORIGIN      
        1 tgtcaaagaa cgagttctca aaaaaatcca cccgggaaag aggggttgag ctcacgccgt
       61 taactcaaca tcctgttggc gccgcctgcg gcgtcgccaa tgcggcgttg cccctatcgg
      121 gtccctgtcc cggccggcgc cgcctgcggc tctgccaacc aggccattgg acccgatatg
      181 cgccttcggc tccgcacgct tcgcgccacg ggtcaacgcc gcattagatt ccagcaacct
      241 ggccgatggg gccggtctcg tgtgtctgct gacccgaaac tcgggacaaa gcctttacgg
      301 cagggttccg cttatcgaag acagactcag aacgctccca atcgatgtcc cccggaaaag
      361 ccttgggcca acgtttaatc acgaaggagg aacgagtgaa cacgtatgaa ctcgacgatc
      421 atctgatcct tcagccggat tcctccaacc gtttcctggt tctcaacgaa acggccggga
      481 tcgtctggaa ggcccatcgg cggggatgct cgctgaccga aatctgcgaa gagttgtcct
      541 gccggttcgg catatcccga aaacaggcgg ccggggacgt cgcgacagtg tttcggacga
      601 tggaatcggc aggcacaatc gattccgaag ccgaagtaaa tgaaaagatg tctttcgtct
      661 ccgccccgga tcgatcgacg tcttgtccgg ccgaatcgga aaaggatctt cgcgttgcgt
      721 attccctttg ggggcggccg ttcgtcgttg cctatggaag ccgacgtctg caggatatgc
      781 ttcattcgta tctgtcgcat ctggtttgca aagaattcgc ggatgcgcct ttgcgctacg
      841 cgctgatgga aagcgaaaaa acgtattttg ttttaaaaaa cggcgacctg ctttcgcgga
      901 caaccgacgc ctacaaggta aagaataccc tgatgctgga cattctggcg cactgccatg
      961 aaaaggagcg attcggcgct gtttggcatg ccgccgccat cacttgcaaa cacgcgtgca
     1021 ttcttttgcc cgccgccagc ggaagcggaa aatccacgct gacggcggcg ctgatccggg
     1081 agggatacgg gtacatggga gacgacttca tcgttctttc cgcatccggt tttgacgcgc
     1141 tggcggcccc tttcgccttg tcgatcaagg aaacaagctg gccggtgctc aagtcgcttt
     1201 acccggaaat cgatgatctt ccggtttatt ctccgtcgca caaaaccgtg aaattccttc
     1261 atccgccccg ggagtctttt ggtttggctt ttcgacgaat gcccgtccat tgcatcatac
     1321 atccaagata tgttcccgga tcttctgaat cgatggaagc aatttcgccg gtggtctcct
     1381 ttcaatcctt gatcgcggca aacgcatggt tggacacgga tgcatccaac atcgccggat
     1441 ttctgcgatg gttggagcga agaaaaagtt acctgatgac gtattcgtcc atttccgggg
     1501 ccagacgtct tctggaggcc gtgttgactg tatgaaccgt attcaggcgg cgaaattttt
     1561 aggcagactg attaccgggc cggaatcgcc ggaagatcgt gaacaagtca accggcgact
     1621 gcgcggggca atgccctggg aacttctggt ggacgtggca ggcggaaatt tgatcacgcc
     1681 ggctttgcat accgctttgt cgtccctcga tgaatacaag aacctcccgg aagacctgcg
     1741 aacgtatctg gcgtatatcc atcgcctcaa ccggtcccgc gacgaggatg tgcaaagcca
     1801 ggtttgcgag atttcacgcc tgtttaacca aatcggcgtg gagccggttc ttctcaaagg
     1861 cgcggccaat ctgtttctcg atatctatcg cgacccggcc gacagaatca tgcacgatgt
     1921 ggatgtcctg gttcccaaag aaaaactgtc cgaatgcacg aacatcttga aagaaaacgg
     1981 ctaccgcggc attttaaaga atttctcctt cacggaagag tcgccgcatt ttttcatttt
     2041 ggtttccagg gagcgcccag tgggcgtgga acttcatcaa tatacgctgc cgaagcagta
     2101 tgcgaccatc tacaccgaca gagatctcgc gcgcgaaagc gtgcggcaga gcgtcgcgga
     2161 cgctgttttc cgtcttcctt cgccgcctta ccacctgctt catcatgttt gccacgcgca
     2221 taagcattgg aaggtttacg gagggccggc gcaaaatagt ttgcgttttg ccgtagattt
     2281 tacgcttctt tgccgttttt atggagatca aatcgactgg atcggaatcg agaaacgctt
     2341 tgaagccggg aaactgagaa atgccctggc ggcatatctt ctggcgacga aggaattatg
     2401 cggctttggc gatccgcttg cccgcgtcgg ggtgagtcgt gcaaaagaac ggtttttgcg
     2461 tggggttttt tttcttttcc ggttccatcc cgtcttcggc gcgtgcaaac ttttccatga
     2521 ataccggctg tccatcaaag ccatggccgc cgatccagca gaacggattg attttctgaa
     2581 gaaattgaac agaaaggaat tttatgctag acagggacaa cggttgatgg gcatctttaa
     2641 aaaagaacac gccttgaaaa atctgtgtga catggatcgg gaaggagccg aatatggtag
     2701 agactaacca tccgaatccg gaaaatcagg ccgtcgcgga cagtgagaaa ccttcggagg
     2761 aactcattac ccgcaaggaa gccttcaaga aaatgggaaa gtacgcggct tattcggccg
     2821 ccgtggtcgc ggttctcctg tcgtctccgg acaaggcggt tgcatcgccg ttgtaataac
     2881 cctacaacgc tacttatgtt ccgcgcagcg atttcctttt tgcatgagaa cggtaagcac
     2941 ggtgatttct cgcctgaatc cgttgaacga tgacaaggag atcctcaccg gcaagaaccc
     3001 tcatgggcaa tagggcttaa aaagggaatc taacaggaag ctgaggacca aggggagcga
     3061 actccccggc ggagtcggaa aacctatccg catgccccat gaggtttgta tcgagctgct
     3121 gtcggccgga ccagggccgc gtgccggggc aggatggtgc gttcggccga tcctcactcc
     3181 gccgtgccca ggatttcccg gatcttccgg gccacgtccc tcatggtaaa cggcttctgg
     3241 atgaaatgga ttccttcctt gagcactcct cgatgggcaa tcacgttctg ggcctggacg
     3301 agttggcgct cgagcccggc cttttccgcc tccacccgtc ttttctcggc aatgttccgg
     3361 accacgatca attggtgggc tcgctctccc agctccaggc gcacgctgtt gcactcgatg
     3421 tccaccagtc cgccgtcttt tcgaagacaa tgcaggtcgg acacctcgtt gcatgactct
     3481 ccggcggccg ccctctcgaa gatctctctc acccgggtcc tctcttcggg aggatggagt
     3541 tcctcgacgc tcatgtgccg gagctcctcc gtggtgtagc ccgccagttc caaggccttg
     3601 tggttgcaat ctacgatgcg atgggttccg caatcggcaa ggaagacggc gtcgttggcg
     3661 tgctgaaaaa gaatccggta tcgtgtttcg ctttcgctca gggcctgttc cgcacgcctg
     3721 cggcgcagga cggagaaggt tagaagaagg accagggcgg tcaggatcgt caaggcggcc
     3781 aaggtggcca ggacgagaat ccggtgggat tcccaaaggg aatcgggctt gttgaggacc
     3841 acgctgtccg cgggcaaagc ggtcaacggg atttggaatc gttggagctg gacatagtcg
     3901 aacatgggcc tggcggtgat cgagaggtcc acggggacct ggtccgcgct ttcccccgcc
     3961 aggacccgca agacctggtc cccggcccgg cgcccgtgtt cccttccgct caagagcctg
     4021 ccgccgacaa ttccatgacc cagccgcgtt tcgtgaacgc cgtagacgga acccgtgcgg
     4081 ccgaagtcag cagccgggtg ctgtcggcaa gagagcggct ctccaccacg gtgtcggtgg
     4141 caaaagagag gatcgaggcc agggaatccg gcggcaggga acccatttcc gccacgagtt
     4201 cgtcgaaagt cccggcggga agaaagcgta cctccacccg ccccgggagg gatcgggcaa
     4261 cgggttccgc ttcccggcgc gcggcacggc cgctcagggt ctcgtcattg accacgtaga
     4321 cccgggtcgt tcgagggtga agggcaaggg aagcctcgag agtgccgccc acgtccatcc
     4381 tttcggctac ccccgtcaca ttcctgtggc cgttcgccaa ctcgaggctg aaatcgctga
     4441 cccctgcaaa gaccaggggc accccgggga acagttcgtc acggaacctc agggccatgg
     4501 tcaaagccgg attgtccagg cagaccacca cgtcgatctt cctgccccgg tatttctcca
     4561 ggagaaaatc cttcacccga accatgtgtt tccgacccgg acggcgcttg gcatccaaga
     4621 actcgatggg gagctcgatc cccggatgaa cctcgtggat tcgctccacg agcccctgca
     4681 tctccccatc ggaccacgca aagccgggat gataggagtt taggatcacc aagcggggca
     4741 cctcgcgggc cgcggcggaa agggccggga agagaagatg ggccgccgca accaggcaca
     4801 gggcaaccag gcacgtcgta cggaaaacga gggaccggcg gatgaggatg cggagtccga
     4861 tcttccggac tcccgcatcg agggcgagtt catccccgga tcggtgttgg atgtttcctg
     4921 cgacaaactt gggacggacg acgggagtct tcatgggcaa ttgggctcga aatggggatt
     4981 ttctccggat tcgactcgac tgaatccgcg gcagaaacta agagatcggg aggaaccttt
     5041 ccaatttccg tgccggctgg gggaaggaat tgtcaaaccc ggtttctcgg atgcagagaa
     5101 ccgaagggtg cccgggactc gagacgttct cccggagacc gaacccgccg ggcagatgct
     5161 ccgattgttc acatccaaat ggaaaggagt gcaccaagtc tgttatgatg agacggttca
     5221 cgggaaatgg accccgctga agcgttcaat cccccaacat ggaggaaggt tcccatggac
     5281 aggaacgatc ccgtcggggc ggcggcggag gcgttgcggg aggccgaggc cgtcctggtg
     5341 acggcggggg cgggcatggg cgtggattcc gggctcccgg acttccgggg aaatcacggc
     5401 ttctggaagg cctacccggc cattgcccgg ctgggaaagt ccttcgtcga gatggccaac
     5461 cccgaatggt tccaccggga cccccggctg gcctgggcct tttacgggca ccggctcaac
     5521 ctctaccgca ggaccgttcc ccacgaaggc ttccgcctgc tcctcgaggt cgcccgaaag
     5581 aaacctcggg gatatttcgt cttcacttcg aacgtggacg ggcatttcca gaaagcgggc
     5641 tacgatgagt cgcgcatcga agaatgccac ggttccattc accatctcca gtgctgcgaa
     5701 ccctgttccg acgacatctg ggacggaacc ggcctggaga tcgacgtgga cgaagacgcg
     5761 ttcgaagccc acgagcccct gccccggtgc cgccggtgcg ggagcatggc ccggcccaac
     5821 atcctcatgt tcggcgactg gtcctgggtc tccgggcgaa ccgaggacca ggggcgtcgc
     5881 ctgtccgagt gggtccgatc cgtggagcgc tcccggtgcc gggccgccgt ggtcgagttg
     5941 ggagccggaa cggccgtcgc cacggtgcgc cgcctctccg agcagttggc ccaccgcctg
     6001 ggatcgcctc tcattcgcat caatccccgg gatttcggca gctccttttc cgggaacatc
     6061 cccatccccg tgggagccgc cgagggaatt cgggccgtcg tttcccggat gggcggtacg
     6121 gcttgaaaga aaggagaacc ggcatggcgc tttacctggt acagcacgga aaggccgtac
     6181 cccgggagca ggaccccgag cagggcctgt cccgagaggg gattcgggac gtggaacgga
     6241 tcgggatggt tgccaaggga tacggagtgc gcgtcgcctc catccagcac agcgggaaga
     6301 gaagggcgcg ccagacggcg gagatcctgg catcgacctt ggattccgtc caggaggttc
     6361 gcgagatccc cggcatcggt cccctggacg atgcgaccct cctggctccc tccctcaaga
     6421 gtcgcgacaa ccggatgctc gtgggacacc tgcccttcct ggagaagctg acctcgaccc
     6481 tcttgaccgg gtccgcggac cttcgcatct tcaaattcca aaacgggggg atcgtggccc
     6541 tggatcaaga cccggagtcc ggatcctggt atatcaagtg gaccttgatg ccccatattt
     6601 cgtgaggggg gaggctttcc attcggactc agggggataa ccaacccagt cccgggagcg
     6661 gactcgaacc ggccgggccg cttcccgcag gggcctcgca ggccctcatc ctgggcgccg
     6721 ccatgttggc cctcttcctg ggggccctgg acgccctcgt ggtggccgcc gccatgccca
     6781 gcatcgtggc ggatctggga ggcatccacc tctacagctg ggttttttct tcctacatgc
     6841 tcacccgggc cacggccctc cccatcttcg gaaagctctg cgacctctac ggcagccgca
     6901 acctgttcct ggcggccgtt gccctcttca tggccagctc ggttctggcc ggtgcggcgg
     6961 attccatgcc ctatctcatc ttctttcgag ccctccaggg gatcggggcc gggggcaatt
     7021 tcgctctggc ctacgtgatc gcggcggacc tggcccctcc ccacaagcgg ggaaaagcca
     7081 tgggctccat ggccttcgtc tggggagtgg ccagcatcct ggggccgtct ctgggcgggt
     7141 ttatggtcaa ctacttctcg tggcgatgga tcttctacat caaccttccc ctgggggctt
     7201 tcgccttcct ttccatcgct ctcttcctca aggaagtccg gcaaaagaaa cccaacgcat
     7261 cggtggacta cccgggcgcc ctggtcctca ccctgtccgt gctggccctc ctcacggcct
     7321 tcctgctggg agagcactgg gaccaatggc tgtctcccac catcccggcc ctcctggtcg
     7381 cctcggcggc cggggtcttt ctgtttgccc ggatcgagaa gcgggcgccc gagcctatcc
     7441 tggccatgga attcttccgc atcccggcct tccgcacggc caacggcgcc gctttttgca
     7501 gcagcttcgc catcttttcc ttgtcggcct tcacccccct ctacatccag ggagtgctgg
     7561 gaaagagccc ggcgaccctg ggcctggcca tgattcccct gagcctggga tggtccctgg
     7621 gcgccctggt ctgcgggcag gtgatccgcc cgcacaggga acggcgcttc agtatctggg
     7681 gcgccctggt cctggccggg agcaccgcca tgatccttcc cttcaccccg ggcactccct
     7741 tgtggctttg ttccgccgtg ctcgccgccg ccgggctggg catggggttc gtgtccatct
     7801 cgaccctcct ggtggtgcaa aacagcctct cggcctccga cctgggcatt gccacttcct
     7861 cccaccagtt cgcccgcacc ttgggcggca ccattgggat cggggcttcg gggagcttct
     7921 tctcgggggc cctcacccga tggacggatc atcccgcaaa cgcccggtgg gctcgggact
     7981 tcccccagtt cttcgggcaa gacctgcccg gcaacctccg gcatctcttc cagtcggatc
     8041 tttcgggaat gcttccccag tctctccaaa atgtgctaag agaagccgtg ctgggcggga
     8101 tcgacatcgt ggtcgggctg gcgctggcag catccgtttt gaacgtctta tgctgttttc
     8161 tactgcccaa agagggccct cgatccccgg atgcggctcc gccccccgtg aatttccccg
     8221 ttagaggtgg catgaaatga tggaagaact ggaaaagaac gaaccgcaag aggaagaaag
     8281 cttcgaagag cttttgaaaa agagccgcgt ggtctccaca cccctgcgca ggggggagaa
     8341 ggtcaagacc gtcgttctca agatcactcc cgaatggacc tttctcgacc tgggcggaaa
     8401 aagcgaagga ttgctgtcca ccaaggaaat ccaggacgag gagggcaatc cgacggtcaa
     8461 agagggcgac gtcctcgagg cctttttcct ttcctcggaa ggcaacgaaa cccggttcac
     8521 cacccgcatc accggcaagg aggctgcggc ggcctatctc gaggacgccc ttgcgggggg
     8581 gattcccgtc gagggagtca tcgagaagga aatcaagggg ggctaccagg taaagctgcc
     8641 ggggaacgtt cgcgggtttt gccccttttc ccagatggga ttgcctcgaa cccggggcaa
     8701 cctggacctc accggtcagc gaatgacgtt caaggtcatc gagtacgagc gggagggccg
     8761 aagggtgctg ctgtccaacc gggccatcct cgaggaggcc cgccgggttc aggtgaacgc
     8821 cctcaaggaa tccctgaagg aagggatgat cgtccggggc accgtggttt ccgtcaagga
     8881 gttcggagca tttctggata tcggggccgt ccagggattg attcccatct ccgagatcgg
     8941 gtacggccgc gtcgaggaca tccaccagtg gctcaaggtc gggcaggaag tcgaggtcat
     9001 ggtggtcaag ctcgattggg agaaggagcg gttttccttc agcatcaagg ccgttctccc
     9061 cgatccgtgg gaagacgtgc ccgccaagta cccggagggg tccgtgcacc gggggaccgt
     9121 gacccggccc atggagtacg gggccttcgt gcagctcgaa ccgggggtcg agggcctgct
     9181 gcccatctcg gagtttcgaa cgggaagaaa ggtcaagcac gcgcgacagc tggtgaccga
     9241 gggccaaagc ctcacggtga aggttctttc cgtagacggg gaagcccggc gcatctctct
     9301 ggggatccag tgggaaagct cggaggaggg ggacgcgccc gtcgagtccg cagccgccta
     9361 tgccgggagc ggatccaaat ccatggggac cctgggggat ctgctgacgg ccaagggggc
     9421 gggcaaagaa cggcgcgaac cgggaaaagg aaagcgcaag cgctgacggg tcgaggcggt
     9481 cgagggcggt gccgcagccg gatgggctac tccccttcca gggcccggcg ctgcatttcg
     9541 gtaaggtagg cgtccagttc ctcgatgctg gcgaagattt ggagagtggg caaggcgttg
     9601 atgatttcga aaaccttctt gatctggggc tgaaggttca cgatgagcag gtttccgttg
     9661 cgctgggcca atccctttct ggccttgagg atcacccgaa tgcccatact gctgatgtag
     9721 tccacccctt tcatatccag ggtgaccacc ctggcccttc cttcgtttag catgaagtcg
     9781 atcctctttt cgaggaccca gtaggtttcg gagtccaacc ggccaaaggg caggaccgtg
     9841 aaaataccgg gctgtttttc ctccgaagtc acattgagag ccatttcccc tccttcttga
     9901 acctcctcga tcctcgaagc ccctcaactc cgcaactgaa agcagagggt caggacattg
     9961 gagtcgtcgt cccgttcgta ccaaacatcg tgggccagct gccgcaccag gagaatcccc
    10021 agacccccca cggtcctttc ggtaaaatcc acgtccaggt cgggagcagg cgcctccagg
    10081 ggattgaacg gcacgcccca gtcccggatt cgaatccggc aggttccgtt ttcgtccgcg
    10141 gtccagtcca cccggatgtc tcccctcgat cccggatacc cgtaggagat gatgttcacc
    10201 agcacctcct cgaggaccag cttcatgtcg cccagcattt cggcaggggc gcccagggcc
    10261 ctggcgggct cgagaacaaa ggcttccagg ggttcaaggg attccatggt agcggacagc
    10321 tgtattgtct tcatgaagac cttgcgtgtc ggtgtcgacc ggcgaaccgg tgcacgggtg
    10381 agtcggcgac ccggcaaaac cgtcggcccg ttttgcccgg gcggcgacct tccggttcaa
    10441 cggagggtag tcaaaaaact cccggttcgc aatggttaaa tggattcccg ggcaacccga
    10501 agcggggaat ccaaccactt gttcagctcg tccccctccc ccgggagcag ggggtaggaa
    10561 acccgctctc cccggtagtc tagaaagacg aggcggtcct cctcccgggc ttccacccgg
    10621 ttgggagtca ggggcacctt cccgcggcct ccgggaagat ccaccaccag gtggggcatg
    10681 gccagcccgg aaatgcggtt tcgcaggcgg gagaggacct cgaggccccg ggccaggggg
    10741 gtccgaaaat gagccgtacc ccgggtgagg tccatctgca tgaggtaata gggccgaacc
    10801 cgcagcctca agagcccctg gaacagctcc cccagcaccg acggatcgtc gttcacccct
    10861 cgaagcagaa ccgtctggct tcccagggga atgcccgcgt ccgccaggat gccgcaagcc
    10921 ttggcggctt cttgggtgag ctcccgcggg tggttgaaat gcaggttcat gaagagggga
    10981 tgatggcggg agagcatccg ggccagctcg agggtgacgg tggccgggga aaacccgggg
    11041 accctggttc ccattcgaac gatccccacg tgggaaatcc tgcggaggga ggaaaggatg
    11101 tgatcgagct tcgacggggc caagaggagc ggatcgcccc ccgagaggag gacgtcctgg
    11161 atttcccg
//
LOCUS       DAOOVC010000126        11137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3802_length_11137_cov_7.405703, whole genome shotgun sequence.
ACCESSION   DAOOVC010000126 DAOOVC010000000
VERSION     DAOOVC010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3802_length_11137_cov_7.405703"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLM79_13820"
     CDS             <1..479
                     /locus_tag="PLM79_13820"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOV87438.1"
                     /translation="ETVLLPLEKAGRVRLEHIRPSGRPMRPREGKVLSVDGDRILFQR
                     RFSKGRYDGLNLPIQPGDYGLTEVQEGAWYVKHSYHRKDGTPIGEYYNINTPVELYPY
                     GARYVDLEVDVIRRAGEKAFLIDQEKLSLLVKEGCIGDRLAIKAMEVAEGLLGSLP"
     gene            510..1820
                     /locus_tag="PLM79_13825"
     CDS             510..1820
                     /locus_tag="PLM79_13825"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOV87439.1"
                     /translation="MDFLPSVSAMARLGAVFFLMLLAIRKKMSLGSAFFLGSLLLGFV
                     FSMSPSGLAVSVFRALLHPKTLSLGIIVCLILVLSRSMELTGRMERLLERFQGLVRAP
                     RLNLIVFPALIGLLPMPGGAVFSAPMVKTLGQSFRLTPRELSYVNYWFRHLWEYWWPL
                     YPGILLTTTLADIDLSLFILVCFPLTLVALGIGFIPLRKLESRSPAEESATGNSPPQV
                     AYFLRELTPFLIVIGLGLGLGQVLSWAAGPGWKDISKEIGLILALLIAIGKVWISGPL
                     TGAQRRKMLTQKSLLQMFSMVAAILVFKGILEDSRAVEAISRELMHWHIPLMPVTAIL
                     PFLVGAVVGITVAFVGTTFPILVSLIQTMGEGPWLAAYLMLALVSGFIGVLVSPLHLC
                     FILSNEYFGTPMGPMYDLLKIPCTLMILAAVLYFFLLRLTPGLS"
     gene            1931..2359
                     /locus_tag="PLM79_13830"
     CDS             1931..2359
                     /locus_tag="PLM79_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87440.1"
                     /translation="MKAKQAWAILSLTWCLLLNLVLAGLLHYLGEQVLLGMSRWMNVL
                     LQITPPGVPEEVQWAYANLGKIVGESRACLAPVVWGLAGVATFVLWWGVYLSGCGYGR
                     RVREEAVSTLPPSETGEDSPIPEGNEPGEDPGNSGPGQQS"
     gene            complement(2367..2582)
                     /locus_tag="PLM79_13835"
     CDS             complement(2367..2582)
                     /locus_tag="PLM79_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87441.1"
                     /translation="MKKIRRPTVHRLHGMLIPGSRYRCRRPDGGWEGNIRSPLVHPEQ
                     LYRQRLPAAAVSFCALFAGLLIASPAR"
     gene            complement(2773..3453)
                     /gene="cysC"
                     /locus_tag="PLM79_13840"
     CDS             complement(2773..3453)
                     /gene="cysC"
                     /locus_tag="PLM79_13840"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOV87442.1"
                     /translation="MTTTKATNITWHHSQVARKDREEILQQRGCTIWLTGLPSSGKST
                     TAYALEHQLIQRGYLAYVLDGDNIRHGLNNNLGFSAEDRQENIRRIGEVSKLMADCGV
                     ITITSFISPYEREREFARTLHKTHGLGFVEVYVHASLATCEKRDPKGLYKKARAGQLK
                     NFTGIDDPYEIPLHPDLVAETERYSPEELACKIIQYLLKLRYLQSEDRRSDLNKAWTL
                     YTASLAAR"
     gene            complement(4093..4473)
                     /locus_tag="PLM79_13845"
     CDS             complement(4093..4473)
                     /locus_tag="PLM79_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87443.1"
                     /translation="MNLGTLAKQLLVVGLGVLSVLVGLLFYRAPDIRAPDHAKVWIHD
                     ETRVYTSPPCFDALLPKLKEGHSYREGTLEEARKLGYAPDKKCQERSGFVQKNRMAVE
                     FLLARFGLLEPLPSRWMPDGNWNW"
     gene            complement(4470..4658)
                     /locus_tag="PLM79_13850"
     CDS             complement(4470..4658)
                     /locus_tag="PLM79_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87444.1"
                     /translation="MPYGAHVSKLEYVTVGGDLKIAVHPKVMEMFHLSPNQDIDMETA
                     QKVLSETERLSGFTRADR"
     gene            5121..6431
                     /locus_tag="PLM79_13855"
     CDS             5121..6431
                     /locus_tag="PLM79_13855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOV87445.1"
                     /translation="MRRCFVSCLLFLFPFCLGSAAHASPPPPVSVSLVKEDVWADRTV
                     REESRGVEITLTFGDECVVIHEDSLSFPEGRLVARDVSPDQLYLVRDLRPMVRRKGYD
                     SLYSHGGFQLAVVKDPAGLTRVKRVSLRPVTGSVVVLERSKVQRLEPNPKVERLLRML
                     DSAHYEQCLSALAEDQPTRYACSDGELDAKNKIKRLFLELGLETEVMAFKNVCGRTCQ
                     KVGGYNVIGIKRGSLRPNEYYLVGAHYDSTSGMPCRMAPGANDNASGTAGVMELARLF
                     SRVETEASLIFVAFSGEEYNQLGSRKYVRRLLNSGLDEKLKAFVVLDMISYYKRNYGI
                     IVEGSNGTTAQKAMVARLARLGRTYTDLAVETTYDYYGSDHEPFLDRGMAGALLIETD
                     WDRYDYYHTRRDKMVYQDIPYAMEALKLAAALLAEGGIIFPADR"
     gene            complement(6844..7221)
                     /locus_tag="PLM79_13860"
     CDS             complement(6844..7221)
                     /locus_tag="PLM79_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87446.1"
                     /translation="MKRKKRRLAHLVAVFFFLVPPCLPPLFSRAAAAANDGNIPGGPP
                     CAERNPGEVAPSAGTESSPRGSFVSADSISTPAVAVYPAGALRVFHRPGCEHHSCTNC
                     TAAYLSREDAVKAGYKPCGICKP"
     gene            7525..8280
                     /locus_tag="PLM79_13865"
     CDS             7525..8280
                     /locus_tag="PLM79_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOV87447.1"
                     /translation="MSKSPLRLSPANAIFLSMLAGLLVGGFFPQAAGYLEPLSTIFIR
                     LVKSLVVPLVFATLVVGIAGHSDLKSVGKMGIKTLLYFEVVTTFALLIGLVMGNLLKP
                     GVGIRLEPLQDLIPEPGRLMTATEVVVNIFPENVADAAARGDLLEVVVFAVLFAAGLY
                     RVDPRKKATLLKFCDALSEAMFKYTAIIMKFAPVGVGAAIAVIVGSKGTAVLWKLGML
                     IFCCYLAIFLFMALVLLPILLLYKIPVRLFLRP"
     gene            8277..8759
                     /locus_tag="PLM79_13870"
     CDS             8277..8759
                     /locus_tag="PLM79_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOV87448.1"
                     /translation="MKGPAVIAFSTSSSEAALPRAMQAMEEPGVSRRIVSFVIPTGYS
                     FNLDGTTLFLSLAALFAAQAAGIELTFQQQVMMGFSLIFASKGAAGIPRASLVVLTGT
                     LASFSIPMSAVALILGVDAVMDMARSATNVIGNCLATAVVARMEGTRFSRPRPASGTR
                     "
     gene            complement(8819..9121)
                     /locus_tag="PLM79_13875"
     CDS             complement(8819..9121)
                     /locus_tag="PLM79_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87449.1"
                     /translation="MDNSLKYGGEHLSRIRTGHEEVEDFHVFSFSDDGKGLREEESRR
                     IFGAFERNETSLGVEGSGLGLSIVQEIAERHGGKVWVEPGNTEGITFYLSISKNLE"
     gene            complement(9106..9405)
                     /locus_tag="PLM79_13880"
     CDS             complement(9106..9405)
                     /locus_tag="PLM79_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87450.1"
                     /translation="MARYRDRLEDLVEERTAALARVNEQLQREIEERRKAEEALRESR
                     QMLESVLDTIPVRLFRKDPDSIFAGSIGPNRTGRRRSRRIGCAFCGLSGISWTTP"
     gene            complement(9499..9834)
                     /locus_tag="PLM79_13885"
     CDS             complement(9499..9834)
                     /locus_tag="PLM79_13885"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV87451.1"
                     /translation="MGASGGVSGPNHGEDVPQENRGGCGECAEKIREAFWMAGADLAR
                     MLYVSPAYEEIWGRSRESLYSNPHCRTEAIHAADREKVHKAVASRMTPGVQCRVSGSA
                     PRRIDPLGA"
     gene            10572..>11137
                     /locus_tag="PLM79_13890"
     CDS             10572..>11137
                     /locus_tag="PLM79_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HOV87452.1"
                     /translation="MADQLFPVPESWTKSAWIDNEKYLQMYKESVDHPERFWGEQAKR
                     LDWFQPWSVVKAGSFDDGVEIKWFVDGKLNVSYNCLDRHLARRGDQTAIIWEADDPAV
                     SKTITYRELHREVCKFANVLKSLGLRRGDRATIYLPMIPELAVAMLACTRIGVIHSIV
                     FGGFSPDSLAGRIQDCRGRVVITADEGLR"
BASE COUNT         2046 a         3439 c         3322 g         2330 t
ORIGIN      
        1 gggagacggt gctcctgccc ctggaaaaag cgggaagggt tcgactggaa cacatccgcc
       61 cttcggggcg ccccatgcgt ccccgggaag gaaaggtcct gtcggtggac ggggatcgca
      121 tccttttcca gcgtcgtttt tccaagggcc gttacgacgg tctgaacctt cccattcagc
      181 ctggggacta cgggttgacg gaagtacagg agggggcctg gtacgtaaag cattcctacc
      241 atcgcaagga cggcaccccc atcggggaat actacaacat caacaccccc gtggagctct
      301 acccttacgg ggcgcgatac gtggacctgg aggtggacgt cattcgtcgg gcgggggaga
      361 aggcctttct catcgaccag gagaagctgt ccttgctggt caaggaggga tgcatcgggg
      421 acagactggc gatcaaggcc atggaggtgg ccgaaggctt gctgggaagc ctcccctaaa
      481 gcgaccgcgg gttttcggat aaggtccaca tggatttcct accgtccgtc tccgccatgg
      541 ctcgattggg ggccgtcttc ttccttatgc tgcttgccat aaggaagaag atgtccctgg
      601 gcagtgcatt cttcttgggt tccctgctcc tgggcttcgt tttcagcatg agcccttcgg
      661 ggttggccgt ttccgtgttt cgagccctcc tccatcccaa gaccctgtcc ctggggatca
      721 ttgtctgcct catcctggtg ttgagtcgta gcatggagct tacgggccgg atggagcggc
      781 tgctcgagcg gtttcaggga ctggttcgag ctcctcggtt gaacctgatc gtctttcctg
      841 ccttgatcgg gctgttgccc atgcccggag gggccgtctt ttccgccccc atggtgaaga
      901 ccctcgggca gtcgttccgc ctgacaccga gggagctgag ctacgtcaac tactggttcc
      961 gccatctctg ggagtactgg tggcccctct acccgggaat cctcctcacc acgaccctgg
     1021 ccgacatcga tttgagcctc ttcattctcg tgtgtttccc cctcaccctc gttgcgctcg
     1081 ggatcggctt catccccctg cgaaagcttg aaagccgttc tcccgccgag gaatcggcaa
     1141 ccggcaactc gccgccgcag gtcgcgtact ttcttcggga gctcaccccc tttctgatag
     1201 tcatcggcct ggggctggga ctgggccagg tcctctcttg ggcggcggga cccgggtgga
     1261 aggacatctc caaggaaatc ggcctcatct tggctctcct catcgccatc ggcaaggttt
     1321 ggatctcggg gcccctgacc ggtgcccagc gcaggaagat gttgacccag aaatccctgc
     1381 tgcagatgtt ttccatggtg gcggccatcc tggtgttcaa ggggattctc gaggacagcc
     1441 gggccgtgga ggcgatcagc cgggaactga tgcattggca cattcccttg atgcccgtca
     1501 cggccatcct cccgttcttg gtcggtgccg tggtgggaat caccgtcgcc tttgtgggga
     1561 ccaccttccc catcctggtt tccctgattc aaacgatggg agaaggtcct tggctggcag
     1621 cctacctgat gctggctttg gtgagcggct tcatcggggt gttggtgtcc cccctgcacc
     1681 tgtgcttcat cctgtccaac gagtatttcg gaacgcccat gggccccatg tacgatctcc
     1741 taaagattcc ctgcaccttg atgatcttgg cggcggtcct ctatttcttc ctgctccgcc
     1801 tgacgcccgg gctttcttga ggaaggggtt caacggcggc gggacgacgg cgatccgccc
     1861 ccggatcgag ggaaccccgg gacgcgacct caacacgcca tccatgttca ccctttgagg
     1921 aaaggaaggc atgaaggcaa aacaagcctg ggcgatcctc tcactgacat ggtgtttgct
     1981 cctgaacctg gttttggccg gattgctgca ctacctggga gaacaggttc ttctgggaat
     2041 gagtcggtgg atgaacgtgc tcttgcagat cacgcccccg ggcgtgcccg aggaagttca
     2101 atgggcctac gccaacctgg gcaagatcgt cggcgaatcc cgggcctgcc tggcaccggt
     2161 ggtctggggc ctcgcgggtg tggcgacctt cgttctctgg tggggagtct acctttccgg
     2221 gtgcggctat gggcgaagag tccgggaaga ggccgtctca acgcttccac cgtccgaaac
     2281 cggggaggat tccccgatcc cggaaggaaa cgagcccggg gaagatcccg gaaacagcgg
     2341 ccccgggcag caaagctgaa gggcgattat cttgcaggac ttgcgatgag caggcctgcg
     2401 aagagggcgc aaaaggagac ggcggcggca ggcagtcttt gccggtagag ttgctccgga
     2461 tggaccaacg ggcttcggat gtttccttcc cacccgccat cgggcctcct gcaccgatat
     2521 cggctgccgg gaatcagcat gccgtgaagt cgatggacgg tggggcggcg aatcttcttc
     2581 aacctctatc ggcaaaatgg acaatccggc gtggaaagcg cctcaggttc cggtgaaggg
     2641 acccgatgag cttcgtcttc tgcggcatca tccgccggga gaagccggtg ggggtgcctg
     2701 ccagggggca cccccacccg ctttccttcg aaggaacgtt cgatcgtttg gcgccggccg
     2761 ccggccggga agtcatcggg cggccaggga ggccgtgtac aaggtccagg ctttgttgag
     2821 gtcgcttcgc ctgtcctcgg actgaaggta gcgcaatttc aaaaggtact ggataatctt
     2881 gcaggccagt tcctcggggc tgtatctttc cgtttccgcg accaaatcgg gatgcagggg
     2941 gatctcgtat gggtcgtcga tccccgtgaa attcttaagc tgtcctgccc gggccttctt
     3001 gtaaagtccc ttgggatcgc gcttttcgca cgtggccagg gacgcatgga cataaacctc
     3061 cacaaatccc aacccatggg tcttgtggag cgtcctggcg aattcccttt cccgttcata
     3121 aggggaaatg aagctcgtga tggtgatgac cccgcagtcg gccatcagct tggacacttc
     3181 tccaatacgc cggatgttct cctgacggtc ctccgccgag aatcccaggt tattgttcag
     3241 cccgtggcga atgttgtccc cgtccagcac ataggccagg taaccccgtt gaatcagttg
     3301 gtgctcgagg gcataggccg ttgtgctctt tcccgaggat ggaaggcccg taagccagat
     3361 ggtgcaccct ctctgctgca gaatctcctc tcgatccttt ctggctactt ggctgtggtg
     3421 ccatgtgatg ttcgtggctt tggtcgtggt cacgtgtgcc tcctgatggt ccctttgaat
     3481 ggattcggat gggtcgcaca aaagtagggg gattgcctga ctgcaccaac gaatcgtgct
     3541 ccgagtgttc gcaacgactt caaatttccg gagaaccact taaatcggtg cataataaga
     3601 cgacatggaa gcctcatgca acggaaaact gacgagctcc aaccgggtcg aatgcagaat
     3661 aagagcagag tcgaacggag gcccaacttg ttgcgggaat ccggcagaag cgtccgtccc
     3721 tccgggtgct cgcccccgcg accccgacgg gctggtgttc tcgggatcgc caagttcgga
     3781 gcagatactc cgcggaggtt cgggaccttt gacgacagga gtgcaggatg accgcaataa
     3841 aaacgctggt gccgaccgtg aggatggcgc cgggattgga tactcatgcc ggcggcgcct
     3901 gtgcggatcg ggacgatagg tcggtccgac cgtcgagact gccgggcatt tcgattcgaa
     3961 cattgcccag cccaaggacg actggatccg tgacgagatg gtgccccgag ccgggctcac
     4021 gagtccaccc gacggaaggg aacctgctaa ggcaccctgc atcgaggtgc atggtgcctt
     4081 tgctgcttta tcctaccaat tccaattgcc gtccggcatc cagcggctgg gaaggggttc
     4141 gaggagtccg aaccgggcca ggagaaattc gacggccatc ctgtttttct gaacaaaacc
     4201 acttcgctct tggcatttct tatccggcgc atagcccagc ttcctggcct cttccagggt
     4261 cccctcccgg tatgagtgcc cctcttttaa tttgggcaac agggcatcga agcagggagg
     4321 cgacgtgtag acccgggtct catcgtggat ccagaccttg gcatggtccg gagcccggat
     4381 gtccggggcg cggtagaaca acaaccccac caggacgctg aggacgccca ggcccacaac
     4441 caggagctgc ttggcaaggg ttcccaggtt catcgatccg cccgcgtaaa gccggagagc
     4501 cgttcggttt ccgacaggac cttctgcgcg gtttccatgt cgatgtcttg attcggcgag
     4561 agatgaaaca tttccattac tttggggtgg acggcaatct tgaggtctcc cccaacggtt
     4621 acatactcga gcttgctgac atgtgcacca tacggcattg ctctttccct cccacgtgct
     4681 gtttggttga attctgcgag gcaaacgacg tgcattgtac ctcatccttc tttgtcttct
     4741 caaggcctgc ccgacgggcg aacgggagga ccccggcccc tccgtttcat gggtcctgga
     4801 gaagaattca acacccgacg aagggtcgca gttctcgttg acatgcagcc gggttcatca
     4861 tagcctcacg gccgtaccct tcgcatcctt ccaggttcca ccccggggtc gggattgagg
     4921 gaaggtccgt tcgcccaggg tcccaggccg gaccgggcaa tcttcgagaa ccgaggtttt
     4981 cgctggagac cgatccagag accgtggata ccgggcgggg acctgcccca tccgtccgat
     5041 cccggacttc gagaacggcg cccgaccttc ctggaccggc tttcaccctc agccgcaatc
     5101 cgaacccagg agagacgcag atgagacgat gcttcgtgtc ctgcctgttg ttcctcttcc
     5161 cgttctgcct cggcagtgcg gcccatgctt ccccgccacc tcccgtgtcg gtatctcttg
     5221 tgaaggagga cgtgtgggcc gaccggaccg tccgggaaga atcccgcgga gtagaaatca
     5281 ctctcacctt cggggatgag tgcgtcgtga tccacgagga ttccctgtct ttccccgaag
     5341 gtcgtttggt ggcccgggat gtgtcgcccg atcagctgta cctggttcgg gatctcaggc
     5401 ccatggtccg gagaaaggga tatgattccc tctattccca cggcggcttc cagttggccg
     5461 tggtgaaaga ccccgccggt ctcacccgag tgaagcgcgt atccttgcgg cccgtcaccg
     5521 gcagcgtcgt ggtcctggaa cggtcgaagg tccaaagact cgagcccaat cccaaagtgg
     5581 agcgactttt gaggatgctg gattcggccc actacgagca gtgcctgtcc gccctggccg
     5641 aggatcagcc cactcggtac gcctgctccg acggagagct cgatgcaaag aacaagatca
     5701 aacgactctt cctggaactg gggctcgaga ccgaggtgat ggccttcaag aacgtctgcg
     5761 ggcgcacgtg ccaaaaggtg ggaggctaca acgtcatcgg aatcaagaga ggatccctgc
     5821 gcccgaacga gtattacctg gtgggggccc attacgactc gaccagcgga atgccttgca
     5881 gaatggcgcc gggggccaac gacaacgcga gcggcacggc gggcgtgatg gaattggcac
     5941 gcctgttttc ccgggtggaa acggaggcgt ccctcatctt tgtggcgttc agcggggagg
     6001 aatataatca gcttggcagc aggaagtacg ttcgacggct gctcaattcc gggctggacg
     6061 agaagctcaa agccttcgtc gtactggaca tgatttccta ctacaagagg aactacggga
     6121 tcatcgtcga aggatccaac ggcacgaccg ctcaaaaagc catggtcgca agactggctc
     6181 gactcgggcg cacctacacg gacctggccg tcgagaccac ctacgattac tacgggagcg
     6241 accatgaacc cttcctggac cgcggaatgg cgggggcctt gctcatcgaa acggactggg
     6301 accgatacga ctattatcac accagaaggg acaagatggt ctaccaggat attccgtatg
     6361 ccatggaggc gctgaaactg gcggccgccc tgttggccga agggggcatc atctttccgg
     6421 ccgatcgttg acggccgagc accgggaact tgattcgaag aaaagcaccc acgaggaggg
     6481 accaccagga acgccttcgg acgagttgcc gccgcgtgga ctgttgccct ggtcgttgcc
     6541 tcgaatccgc cttcgggact ttccaattat gagcacggac gggccggtgt caacgaacac
     6601 ccgacccagc gtaccgccgc ggtcgattgg aggaagggtg ttcaccctgg atgcggccga
     6661 atttgctgcg ctccccccgg gggccccagc cttcgcatcg ggggcgcacc tccctggaaa
     6721 ttcggaccca tcgatccgtc gcgggtggac tgaggagtcg ggagggtttc aacccgctga
     6781 gacgggtcga gaaagggaga aatcccggga agtagcttta tcatgagcgc cgtgcccgcc
     6841 gggttagggc ttgcagatcc cgcagggctt gtagcccgcc ttcaccgcat cctcccggct
     6901 gaggtacgcc gccgtgcaat tggtgcagct gtgatgctca caccccggac ggtggaacac
     6961 ccgcaaggcg cccgccggat agacggccac cgccggggtc gagatcgagt cggcggaaac
     7021 gaaggatcct cggggagaag attccgtccc tgccgacggt gcgacttccc cgggatttct
     7081 ttcggcgcag ggagggccgc cggggatatt tccgtcgttc gcagccgccg cagctctcga
     7141 gaaaagaggg gggaggcaag gcggaaccag gaagaaaaac accgcgacaa gatgggcaag
     7201 acgcctcttt ttgcgtttca tatcccccaa gtcttcttac agagcgtccc gaggaggcgg
     7261 gtcgcgggat gagcgttttc cacagaagac gctccccaaa ggagcggtcg gcttcggcat
     7321 ccgagtcatc atgccaaacg ggtggaggat ctgcaacggg gaaatccccg gcgaggggga
     7381 gcgggggcta ccggccggat tcccttgact tcttgccgct tcggatcgta ccaataggtc
     7441 cagcaaccct ttcgagtccg ggtcctggcg gtggcggacc acccttgccg tctcaggatc
     7501 ccggcatatt ccggagaccc gctcatgtcg aaatcaccgt tgcgtctttc gcccgccaac
     7561 gccattttcc tcagcatgct ggcgggattg ctcgtcggag gattctttcc ccaggctgcc
     7621 ggctaccttg aacccctgag caccattttc attcgactgg tcaagtcact ggtcgttccc
     7681 ctcgtctttg ccaccctggt ggtgggcatc gccggtcaca gcgacctgaa gtcggtggga
     7741 aagatgggga tcaaaacgct cctctatttt gaagtggtga ccaccttcgc cctgctcatc
     7801 gggcttgtta tgggcaacct gctgaagccg ggggtgggca tccggctcga acccttgcag
     7861 gacctgattc ccgagcctgg ccgcctcatg accgccaccg aagtggtcgt caacatcttc
     7921 cccgaaaacg tcgcggacgc ggccgcccgg ggcgatctgc tggaagtggt ggtctttgcc
     7981 gtccttttcg ccgcggggct ttacagggtc gatccacgga agaaagcgac gttgctgaaa
     8041 ttctgcgacg ccctgtccga ggcgatgttc aagtatacgg ccatcatcat gaaattcgct
     8101 cccgtcgggg tcggcgcagc catcgccgtc atcgtgggca gtaagggaac ggccgtcctg
     8161 tggaagctgg gaatgctgat cttttgctgc tatcttgcca tcttcctttt catggcgctg
     8221 gtgctgcttc ccatccttct tttgtacaaa atccccgtac gccttttttt gaggccgtga
     8281 agggcccggc ggtcatcgcc ttctcgacga gcagctccga agcggcgctc ccccgggcaa
     8341 tgcaggccat ggaggagccg ggagtgtccc ggcgcatcgt ttccttcgtg atcccaaccg
     8401 gttacagctt caacctggac ggaacgaccc tcttcctctc cctggccgcc ctgtttgccg
     8461 cccaggcggc aggaatcgag ctgaccttcc aacagcaggt catgatgggg ttctccctga
     8521 tcttcgccag taaaggggcc gcaggcatcc cccgtgcttc cctggtggtg ctcaccggca
     8581 cccttgcctc cttttccatc cccatgtccg ccgtggcctt gatcctgggc gtggacgccg
     8641 tcatggacat ggcccgctcg gccaccaacg tcatcggcaa ctgcctggcc accgccgtgg
     8701 tggcgcgaat ggaaggaacc cggttttcca gaccccgccc cgcctccgga acccggtgag
     8761 cccgcaagtc cctgcagcct caccggcagg gccgaccgcc cgagcgatgc gccctttcct
     8821 attccaggtt cttcgagatg gaaaggtaga aggtgatccc ctctgtgttt cccggttcca
     8881 cccatacttt tccgccgtgt cgttcggcga tttcctgaac gatggagagc cccagacccg
     8941 acccctcgac tcccagagac gtctcgtttc gctcaaatgc cccaaagatt ctcctggatt
     9001 cctcctctct gagccccttc ccatcgtcgc taaatgaaaa gacatggaaa tcttccacct
     9061 cttcgtgccc ggtccggatc ctgctgaggt gctccccccc gtacttcaag gagttgtcca
     9121 tgaaattccg gaaagcccgc agaatgcaca tccgatccgc cttgatctcc ggcgcccggt
     9181 ccggtttggc ccaatcgatc ccgcgaagat cgaatccggg tctttccgga acagcctcac
     9241 gggaatggta tccagaaccg actcgagcat ttgccgcgat tcgcgcaggg cctcctccgc
     9301 tttcctgcgt tcctcgattt cacgctgcag ttgttcgttc actcgagcca gggccgcggt
     9361 ccgttcttcc accaggtctt cgaggcggtc tcgatagcga gccaactctt cttgggcaag
     9421 ctttcgttcc gtgatgtcct cgacgattcc ggcaatgcgg aaacgccgcc cgacgaatcc
     9481 cggatgggga atccccggtc aagcacccag cggatcgatc cgtcggggcg cactacccga
     9541 tactcgacat tgaacccccg gggtcatccg ggaagccacc gctttgtgga ctttctcccg
     9601 gtcggccgca tggatcgcct cggtccggca atgaggattc gaatacaagc tctcccgaga
     9661 acgcccccag atttcttcgt acgcggggct gacataaagc atccgggcca ggtccgcacc
     9721 cgccatccaa aaagcttccc ggatcttttc ggcacattcg ccgcagcctc cccggttctc
     9781 ctgcggcaca tcttccccat ggttgggccc gctcacgcct ccggatgctc ccatttgagg
     9841 cttcctccct tgaacgaaat tggacttcca cccattcggg cctcccgaaa tgccgggagc
     9901 ctccttgtgc cgatgttgtg gtggattgga aaactcacga aagctccctc gcccccgctt
     9961 gggaaaagag ggacttccgt cacccacacg ccctcccgcc gaggcgcggt ccgggtgcga
    10021 aacccggcag cccttgctct ttctcgattg ggcgcaccca cggaggaccc gggttgcctg
    10081 acggagaaac cgaaatccgc atccaagtag gctgcttccc ggatcgacgg tcgtcgggcc
    10141 ggggaacgaa cccttcgttt tccacacggc gttttccaca cggcgctttc cactcggcac
    10201 gggcttctga ttcccggaaa ctcccgggaa cggggcccgt ctcgactacc tgcccggcag
    10261 gccgccctcg gaattcctgc cgggaaaggg gtttcgcccc ggccgaaatt tttcggaaac
    10321 cgacaattat gcccttgaca aagctcgtca tttcatgaag aaatgcccgc ccgtggattg
    10381 ttcgcgattc cccgtccacc gcctgcgaca tctcccgtta cgagccaatt tgaatcccgc
    10441 gttgctcaga ttggcagctg ttgggcagcc tgcttctgcc tgtcgttcct cgcccttccc
    10501 acggccccgg gcccaatgtt gcgtcggcag tcgcggtgta accatcccat ttaaccttga
    10561 aggagcagac tgtggcagat caattgtttc ccgttcccga atcctggacg aaatcggcct
    10621 ggatagacaa tgaaaagtac ctgcagatgt acaaggagtc ggtggatcat cccgagcggt
    10681 tttggggaga gcaggccaag cgcctcgatt ggttccagcc gtggtccgtg gtgaaagccg
    10741 gttccttcga cgatggagtt gaaatcaagt ggttcgtcga cggcaagttg aacgtcagct
    10801 acaactgcct ggatcgccac ctggcccggc gtggggacca gacggccatc atctgggaag
    10861 ccgacgaccc ggcggtgagc aagaccatta cgtatcgaga gctgcatcgc gaggtctgca
    10921 aattcgccaa cgtcctaaaa tccctggggc tgcgccgggg ggatcgggcc acgatctatc
    10981 ttccgatgat tcccgagctg gccgtggcca tgctggcctg cacccggatc ggcgtcattc
    11041 attccatcgt cttcgggggg ttctcacccg attccctggc gggacggatc caggattgcc
    11101 ggggcagggt ggtgatcacg gcggacgagg ggttgcg
//
LOCUS       DAOOVC010000127        11037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3841_length_11037_cov_6.267256, whole genome shotgun sequence.
ACCESSION   DAOOVC010000127 DAOOVC010000000
VERSION     DAOOVC010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11037
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3841_length_11037_cov_6.267256"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /gene="dnaK"
                     /locus_tag="PLM79_13895"
     CDS             complement(<1..1451)
                     /gene="dnaK"
                     /locus_tag="PLM79_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOV87453.1"
                     /translation="MGKVIGIDLGTTNSVVAIMEGKEPKVLPNAEGGRTTPSMVAITD
                     SGERLVGQVAKRQAITNPENTIFAVKRLIGRKFDTKEVQQDVKILPYKIVKAANGDAH
                     LRIRGRDYSPTEISAFILTKMKQTAEDYLGEKVNEAVITVPAYFNDSQRQATKDAGKI
                     AGLNVLRIINEPTAASLAYGLDRKKDEKIAVFDLGGGTYDISILEIGEGVFEVKATNG
                     DTHLGGEDFDQRVIDWLATEFKKDYGIDLRNDKMALQRLKEAAEKAKCELSTTMETEI
                     NLPFITADASGPKHMNIKLTRAKLESLVEDLVERVVPPMEQALRDAGLSRSDIDEVIL
                     VGGMTRMPRVQQKVSEFFGKEPHKGVNPDEVVAIGAAIQAGVLKGDVKDVLLLDVTPL
                     SLGIETLGGVMTRLIERNTTIPTRKSQIFSTATDSQTAVSIHVLQGEREMAGDNKTLG
                     RFELVGIPPAPRGVPQIEVTFDIDANGIVHVSA"
     gene            complement(1571..2167)
                     /gene="grpE"
                     /locus_tag="PLM79_13900"
     CDS             complement(1571..2167)
                     /gene="grpE"
                     /locus_tag="PLM79_13900"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOV87454.1"
                     /translation="MIEASKEREAMEKTEMRQASPPQEQPGNEGVTVSAVGESEAGQI
                     SDPRDEEIKELKDRVLRLAAEMDNTRKRLEREKSDGISFANECLLRELLPVMDNLERA
                     IEHAEKETDCQSLISGVRMTMKGFLDVLAKFGCAPFESAGKAFDPNYHEAILQREDPE
                     KPGKTVLEEYQKGYTLHDRLLRPALVVVSGSGNQEGEG"
     gene            complement(2256..3302)
                     /gene="hrcA"
                     /locus_tag="PLM79_13905"
     CDS             complement(2256..3302)
                     /gene="hrcA"
                     /locus_tag="PLM79_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOV87455.1"
                     /translation="MIELSGRDRQVLEAVVSDYIQSGEPVGSRTVSKRYELRVSPATI
                     RNVMADLEERGLLSQPHTSAGRVPTEKGLRIYLDTLMRSRTLGKEERARIRRAFQENK
                     SDIQAILQQTSRILSSFCRQAGVVLWPSWKATRLKHIEFVRLRPNQVMVILISKTGLV
                     KHSLLEWPEDIKQEELDKYGRYLNERFQDLPLGEVKERILEEMVKEKVLFDALYSRAL
                     EVTQRFFESSLENSEVYIEGQINLLENPEFSDVERLKGILEAFENKSRIVRLLDMSLK
                     VSSGVQIILGPESEFQELQQISLISSPYRHGDTLLGVLGVIGPLRMDYPRIIPVVEFT
                     ASLLSQLLNDPDEI"
     gene            complement(3430..3651)
                     /locus_tag="PLM79_13910"
     CDS             complement(3430..3651)
                     /locus_tag="PLM79_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87456.1"
                     /translation="MKRMWIRLKEGQDLTAVKRMLGELGRIEEDPDDPRRVVLTFRNQ
                     SDSGILMAGYQMSENYMEYFDDFDMIREE"
     gene            complement(4014..4646)
                     /locus_tag="PLM79_13915"
     CDS             complement(4014..4646)
                     /locus_tag="PLM79_13915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV87457.1"
                     /translation="MKKTDKVPLVIESEIFLASEESKTHKARSRILGGRHGDFILLED
                     PVYRVNERLSVPLEGTLVCSYFHEGDVYHFRSRIRKDLGDGLVLMEYPTRFQVEKVRK
                     HHRIFVDIETRLYLDGAKDALGATTTDISEGGCCLVVAALLPVVRNSGCRIELILPDN
                     QQITGLRAKIRNITFSTLKKTTQLGLQFLGPGDQLAKISSFCRFCMFFKV"
     gene            complement(4820..6388)
                     /gene="murJ"
                     /locus_tag="PLM79_13920"
     CDS             complement(4820..6388)
                     /gene="murJ"
                     /locus_tag="PLM79_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOV87458.1"
                     /translation="MGPWNRRQEMGWAAVIMGVSVLLSRFMGLIRDKIISYLFGATRE
                     TDLYFAAFVVPDFINYLLAGAYFSITLIPLLAEHFHKDEEEGWRFLSTVLTWIFLTAA
                     ALTLVAMILAPQLAFFAAPGLDDAALRRLARFLRIVLPAQVCFLLGSCFMAVLYLRKQ
                     FLLPALVPLVYNSMIILGGVILRRRGMEGFCWGVLTGAFLGNFLLPLWAVNKEGGVKF
                     RPRLTHSGLKGFLLLALPLMLGQSIVVLDEQFTRIFASLAGIGAISWLNYGRRIMMVP
                     VGVVAQAAGVASYPFLADLVARKEFPKFHETLNRALLNVCVLLLPLSFWMITVSEPII
                     VLIFQQGKFGAADSVQTTRVLQILLLAVLFWGGQQIVARGYYARQDTLTPAVLGTVTS
                     LVSVPVFYLAGLHYGAPGVAGASGVSVILYTLLLSGWWGRRFGWATFRGLQAALGKTF
                     LVALVCCGPSYWIVRWQFIRDAAHPYLWALGTIGVTALVYGGTFLVLGRLLLGTLLDP
                     FLEKAGPLGRRLLR"
     gene            6766..6842
                     /locus_tag="PLM79_13925"
     tRNA            6766..6842
                     /locus_tag="PLM79_13925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6800..6802,aa:Arg,seq:acg)
     gene            complement(7139..7930)
                     /locus_tag="PLM79_13930"
     CDS             complement(7139..7930)
                     /locus_tag="PLM79_13930"
                     /inference="COORDINATES: protein motif:HMM:NF021627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HOV87459.1"
                     /translation="MTDTLGFIAGLPEDKGRPTAVPTEFQIFPHGRVTLVDGEPFIVD
                     DAAMSAVISAFDSRGLDMVIDYEHQTEGGEYSSPDGKAPAAGWIKSIENRGEDGLWAK
                     VEWTEAAADLLAKKEYRYFSPVFLVSKGERRLVELLRVALTNAPRLNWIKPIVAKNAN
                     PTEKEVEMEFLKRIAKQLGLPESVTQDQVLETIRKVQDSGQEFLKLTAKETGIPETSN
                     SCAGANRVGARGVYGRKEIHCRNSSGRGGVAPFWSTSTCTGILRC"
     gene            complement(8038..9291)
                     /locus_tag="PLM79_13935"
     CDS             complement(8038..9291)
                     /locus_tag="PLM79_13935"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HOV87460.1"
                     /translation="MDYPSTEILAQGLPFQEAIDFFRQKVRLPTRTWTDIWEGMHSRA
                     FVVAGAMKDDLLSDFQAAVLMGIEKGITLNEFRKDFDSIVKRHGWSYKGGRGWRSSVI
                     FNTNMRTAYQAGHYKQMTDPDVLEARPYWRYSAVMDGRTRTQHVAWHNTVLRHDDPWW
                     RTHYPPNGWGCRCTVVNHSGPEVERMKAEGEKLREEAPEMDWQEKEIRLSDGTTRTVR
                     VPNGIDPGWAYNVGETAWGSQLPEEVMDAWRRRGAAAWERMTSGDRLSYGRPRELPLD
                     ITQTSAVSPMQSKAEAEKYLQGILGGDEKIFMAGADLKMPVLVNARSLSEHIPLDRSA
                     FLPFFPELIQEPYEVWLSFEKHKGTGKVVLRARFLKAIQLEKGRGLFMSAQTVRGMLE
                     AWTFFTSKGWKYLESQRRGLLIFGR"
     gene            complement(9264..10889)
                     /locus_tag="PLM79_13940"
     CDS             complement(9264..10889)
                     /locus_tag="PLM79_13940"
                     /inference="COORDINATES: protein motif:HMM:NF017850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HOV87461.1"
                     /translation="MLYDQFGRPIQERDIRSPETRDISVVQVRDRWSSYPSYGLTPQR
                     LARILKEADNGDVVRQAELFEEMEEKDAHLASQLQTRKLSVQGLSWEILPAAEDATSK
                     EIAEFCNFAIDGLPDFDDHILDLLDAISKGYSMMEILWDVSAGQAAIRGLRYVHPKKI
                     TFRDSVYPRVLTDDEPIRGIDPPPYKFAYHRYKARSGYDTRAGIMRVCAWMYLFKNYA
                     VKDWVSFSEVYGMPIRVGKYEPGAGKADKDALLSAVRMLGSDAAGIISKATEIEFIET
                     QKGSGSSGQLIYEGLARFCDAQMSKAILGQTLTSEAGGEKGRGSLALGRVHGDVRQDL
                     VEADCRALAKTITHQILRPLVGFNYGWDAPVPCFRFLYEPPEDLEAYAAIYKILCADM
                     GQPVSQEHLSARFKIPLPQPGETPLAPRRAAGPSPKNEPGGKQPGDAAEDIAKLIAKL
                     AASNRDAADIIADQLQDRAMLEMTGMTDRIRDLVDSAASLEEVRDRLINLYADIDPTR
                     LGEIMQQAFALADLSGRFDAEGGRVWTTHLQRS"
BASE COUNT         2168 a         3404 c         3112 g         2353 t
ORIGIN      
        1 ttggcggaca cgtgcacgat gccgttggcg tcgatgtcga aggtcacctc gatctggggg
       61 actccgcgcg gagccggggg aattcccacc aactcgaatc tgcccagggt cttgttgtcg
      121 cccgccatct cccgctctcc ttgcaggaca tggatggaca ccgccgtttg gctgtccgtg
      181 gccgtcgaga aaatctggct ttttcgggtg gggatggtgg tgttgcgctc gatcagccga
      241 gtcatgactc cacctagagt ctcgattccc agggacaacg gagtgacgtc cagaagcagc
      301 acatccttga cgtctccctt caggaccccc gcctgaatgg cagcgccgat ggccaccacc
      361 tcatcgggat tcacgccctt gtggggctct tttccgaaga actccgatac cttctgctgg
      421 acccggggca tacgggtcat tccacccacc aggatgacct cgtcgatgtc cgaccggctg
      481 agccccgcgt cccgcaaggc ctgttccatg ggcggaacca ctcgttccac caggtcttcc
      541 accagggatt ccagtttggc ccgggtcagc ttgatgttca tgtgcttggg ccccgaggca
      601 tccgcagtga tgaagggaag gttgatttcc gtttccatgg tggtggagag ttcgcacttg
      661 gccttttccg cggcttcctt caaccgctgg agggccatct tgtcgttgcg gaggtcaatg
      721 ccgtagtcct tcttgaattc ggtggcaagc cagtcgatga cccgttggtc gaaatcttcg
      781 cctcccaggt gagtatcccc gttggtggcc ttcacttcga agaccccttc cccgatttcc
      841 aggatggaga tatcgtaggt gccgcccccc aggtcaaaaa cggcgatctt ctcatccttc
      901 tttcgatcca ggccgtaagc aagggaggcc gccgtcggct cgttgatgat tcgaaggacg
      961 ttcaaaccgg cgatctttcc ggcatccttg gtggcctgtc gctggctgtc gttgaaatag
     1021 gcgggcacgg tgatgaccgc ctcgttgacc ttctccccca agtaatcctc cgccgtctgt
     1081 ttcatcttgg tgaggatgaa cgcggagatc tccgtggggc tgtagtcccg gcctcggatc
     1141 cgcaggtgtg cgtccccgtt ggcagccttg acgatcttat agggaaggat cttgacgtcc
     1201 tgttgaactt ccttggtatc gaacttgcgc ccgatcaacc ttttcacggc aaatatcgtg
     1261 ttctccggat tggtgatggc ctgtctcttg gccacctgcc cgaccagccg ctcgccgctg
     1321 tcggtaatgg ccaccatgga cggggtcgtt cgacccccct cggcattggg caggaccttg
     1381 ggttccttgc cctccatgat tgccaccact gagttggttg tacccaaatc gatcccgatc
     1441 actttaccca tgtgatttgc ctcctctgtt cacagccgcc gatctcgatg agtgcgtgct
     1501 tgtatgaccc tgatcctttt atagtgaaat ctaatcttct atccacaggg cttcgtcccc
     1561 gactcatgac tcacccctct ccttcttggt tgccacttcc cgataccacg acgagggcgg
     1621 gccgaaggag acgatcatga agcgtgtacc ctttctggta ttcctccagc accgttttgc
     1681 cgggtttttc cgggtcttcc ctttgcagga tcgcctcatg atagttggga tcgaacgcct
     1741 tccccgcgga ttcgaagggc gcacaaccaa atttcgccaa aacatccagg aagcccttca
     1801 tggtcattcg gacccccgaa ataagacttt gacaatcagt ctccttctcg gcgtgctcga
     1861 tggccctttc caggttgtcc atcaccggga gaagctcgcg aaggaggcat tcgttggcga
     1921 acgaaatccc gtccgacttt tctcgttcca atcgcttcct ggtgttatcc atttccgcag
     1981 ccaagcgaag gacccggtcc ttgagctctt tgatctcctc gtctctgggg tccgatatct
     2041 gcccggcctc gctttccccc acggcggaga cggtcacacc ctcatttccc ggttgctcct
     2101 gtggcggaga agcctgtctc atttccgtct tttccatggc ttcccgttct ttcgatgcct
     2161 caatcattca gacaaatcct ccttcagaac cctggcttta gggaattccc atagggacta
     2221 cccccgtgtc gtttccccgg ccccctgcac ccccttcaga tctcatccgg atcgttgagc
     2281 aattggctca aaaggctggc cgtaaactcc accaccggaa tgatccgagg gtaatccatc
     2341 cgcaacgggc caatgacccc cagcaccccc agcaaagtat ccccatggcg ataaggggaa
     2401 gagatgagac tgatttgctg gagttcttgg aactcgctct cggggcccag aatgatctgg
     2461 acgccggacg aaactttgag agacatatcc aagagccgga cgatccggga cttgttttcg
     2521 aaggcctcga ggattccctt gagcctttcc acgtccgaga attcggggtt ttccagcagg
     2581 ttgatctgcc cctcgatata gacctcactg ttttccaggg aggactcaaa gaagcgttgc
     2641 gtgacctcca aggcacggga atacagtgcg tcgaaaagca ccttttcctt gaccatttcc
     2701 tccaggatcc gctccttcac ttcgcccagg ggcagatctt gaaaacgctc attgagatac
     2761 cggccgtact tgtccagctc ctcctgcttg atgtcttcgg gccactccag caagctgtgt
     2821 ttcaccaatc cggtcttgga gatgagaatg accatgacct ggttgggtct caggcgcacg
     2881 aattcgatat gcttcagccg ggttgccttc caactgggcc acaggaccac cccggcctgc
     2941 cgacagaagc tggacaaaat ccgggatgtc tgctgcaaaa tggcctgaat atccgacttg
     3001 ttttcttgaa aagccctgcg gatccgggca cgctcctcct tccccagtgt cctggacctc
     3061 atgagagtgt ccaggtagat acggagtcct ttctccgtgg gcacccgccc cgccgaagtg
     3121 tggggctggg agagcaagcc tcgctcttcc aggtcggcca tgacgtttcg gatggtggcg
     3181 gggctcaccc tcagttcgta ccgtttggag accgtacggg aacccaccgg ttcgccggat
     3241 tggatgtaat ccgacactac ggcttccagt acctggcgat cccgccccga caattcgatc
     3301 actggagtct ctcccggatg cgattagcac tctgccaaac tgagtgccaa gctagtgctg
     3361 acgccatctt ttgtcaaggt tccgactgcc ccctccccca tcgttaggcc cggctgtccg
     3421 gcaacaaagc tactcctccc ggatcatgtc gaaatcatca aagtactcca tgtagttttc
     3481 cgacatctga tagcccgcca tcaagatccc gctgtccgac tggttgcgga aagtgagcac
     3541 cacccgccgc gggtcgtcgg ggtcctcctc gatacgcccc aattcaccca gcatccgctt
     3601 cacggcagtc aggtcctgtc cttccttcaa ccgaatccac attcttttca tggttcctac
     3661 ctcctcacaa cccattcacc gatctgccgg aagcaaagag cgcttttccc gcacttcgtt
     3721 ggattccaat aaaggataaa gttcaaaaag gccctgtcaa ggaaggatac ccgcgaggag
     3781 tccgcaacag gaaggagccg gggcaaaaag tgccccgaaa ccgcaccgtc gcccttggtc
     3841 gtccatccgg ccaaacgttc cggaccgaac gcttgccggt gtccgaaaga ctatgggggc
     3901 atttcccggc cccggtccaa atcccggttc atccttggtt cccggggccg atctccacgg
     3961 ggaggcgcaa ggggggtttc gaggtccccc tcaggaccat cggcctctcg ggttcatacc
     4021 ttgaaaaaca tgcaaaatcg gcaaaaggaa gaaatcttcg ccaattggtc cccggggccc
     4081 aagaattgga gtcccaactg cgtcgtcttt ttcagggtgg aaaaggtgat gttgcggatc
     4141 tttgcccgga gccccgtaat ctgttgatta tccggaagta tcagctcgat tcgacacccg
     4201 gagttgcgca ccaccgggag cagcgcagcc accacgagac aacatccccc ttcgctgatg
     4261 tccgttgtgg tcgctcccag cgcatccttc gcaccatcca gatacaagcg ggtttcgata
     4321 tccacgaaga tccggtgatg cttccgcacc ttctccacct gaaaacgggt ggggtattcc
     4381 atgaggacca gtccgtcgcc caggtctttg cggatgcggc ttcgaaagtg gtacacgtcc
     4441 ccctcgtgaa agtacgaaca aaccaatgtc ccctccaatg gaacgctcaa gcgttcgttg
     4501 acgcgataca cgggatcttc caaaaggatg aaatccccat ggcgcccccc gaggatccgg
     4561 gaccgggcct tgtgggtctt gctctcctcc gatgccagaa aaatctcgct ctcgatcacc
     4621 aaagggactt tgtccgtttt cttcatggga agccacgcct gtgcctaagg cctgaacgag
     4681 aaaaagatac gggccaatct ttcacgggtg caaaatagct ttccggaccg gcaaagtaaa
     4741 ggcgaatatg ggaagctctc cgcccgaact ccccgctgaa cggagcggcg agatcgccga
     4801 aaagataccg caaccgggtt taccggagga gacgccggcc caggggtccg gccttttcca
     4861 agaagggatc gaggagagta ccgagaagga gacgtcctag gaccaggaat gtcccgccgt
     4921 ataccagggc ggtcacgccg atggtcccca gggcccaaag ataagggtgc gcggcatccc
     4981 ggatgaattg ccaccgcaca atccaataac tgggtccgca gcacacaaga gccaccagga
     5041 aggtcttccc cagggccgcc tgcaaccctc gaaacgtcgc ccaaccgaaa cggcgacccc
     5101 accagccgga gagcagcagg gtgtagagta ttaccgacac accgctggcg cctgccacgc
     5161 ccggggcccc gtagtgcagg ccggccaggt aaaagaccgg caccgacacc aggctggtca
     5221 cggttcccag gacggcgggg gtgagagtat cctgccgggc atagtagccc ctggccacaa
     5281 tctgttgacc tccccagaac aggaccgcca ggagaagaat ctgcaagacc cgcgtggttt
     5341 gaaccgagtc ggccgccccg aattttccct gctgaaagat caggacaatg atgggttccg
     5401 agacggttat catccagaaa gacaggggaa ggagcagaac gcagacgttg agaagcgccc
     5461 ggttcagggt ttcatggaat ttgggaaatt ccttcctggc cacgaggtcc gccaggaaag
     5521 ggtaggaagc gacccctgca gcctgggcga ccacccccac gggaaccatc atgattcgcc
     5581 gtccgtagtt gagccagctg atggccccga ttccggcaag agaggcaaag atccgggtga
     5641 actgctcgtc cagaacaacg atggattggc ccagcatcag cggcaacgcg agaaggagaa
     5701 accctttcaa tcccgagtgt gtcagacggg gccgaaactt gaccccgccc tccttattca
     5761 ccgcccagag gggcaggaga aaattcccca ggaaagcccc cgtcagaact ccccagcaga
     5821 acccttccat tccgcgacgt cgaagaatca ccccccccag aatgatcatc gagttgtaaa
     5881 ccaggggcac cagggccggc agcaggaact gcttgcgcag gtataagacc gccatgaaac
     5941 aggaacccaa cagaaaacag acttgggcgg gcaggacaat cctgaggaag cgggccaagc
     6001 ggcggagggc cgcatcgtcc aggccgggag ctgcaaaaaa agccagttgc ggggccagaa
     6061 tcatggcgac cagcgtcaaa gccgcggctg tgagaaaaat ccatgtcagg acggtggaaa
     6121 gaaacctcca accttcctct tcgtccttgt gaaaatgctc cgccagcaag gggatcaggg
     6181 tgatggaaaa gtaggctccc gccaggagat agttgatgaa atcgggaacg acgaaggcgg
     6241 caaagtacag gtccgtctcc cgggttgctc caaaaaggta ggaaatgatc ttgtcccgga
     6301 taagacccat gaagcgggag agcagcacac tgactcccat gatcacggca gcccagccca
     6361 tttcctggcg acggttccac ggacccatag aggagttctc tctcttctcc cggggtgtct
     6421 tccggctcgg gttctttctt cagcggtctc cccctccgat ccgtttcaga ctattccaaa
     6481 tccccatcgg tgtcaaaaac tgctcccggc ctgtcgctcc cgacggggtc gggccggtgc
     6541 gcatagtcca ccccgggaga tgggtgccgt ctcccaacgg gtccttcctg gaacgggact
     6601 cgcggcgggg ctccctccga gattcgtccg ccgccccaac cggaacgagg acgtgcggac
     6661 aatgtccgcc cataggctcg tccggcccgt gccgcaaact tttccgggag ttttcccttg
     6721 cttgcccggg ggaattttgt tagatcaaag gccaaccaat cggacgcgcc cgtagctcag
     6781 ctggatagag tgccggacta cgaatccgta ggccacaggt tcgaatcctg tcgggcgcac
     6841 caggaaaatc aaggggttac ggagaatccg ccgtagcccc ttttgggtgg tgtgtaaatg
     6901 ctgaagtttg cagtttagcc cctcgtcccc tgttttttgc atggatgcac cgtatcgggc
     6961 gtccggagca tacttcctga atgccttgtt ggcgccgcct gcggcgtcgc caatgcggcg
     7021 ttgcccctat cgggtccctg tcctggccgg cgccgcctgc ggctctgcca accaggccat
     7081 tggatccgat gtcactcgct tgcatgcgcc ttcggctccg caagcttcgc gccacgggtc
     7141 aacaccgcaa aataccggtg catgtagatg ttgaccaaaa aggggcgaca ccacccctcc
     7201 ctgaggagtt cctgcagtgg atttctttcc tcccgtatac accacgtgcc cctactcggt
     7261 tcgcccctgc acaggagttg gatgtctccg gaatccccgt ttcttttgca gttagtttga
     7321 gaaattcctg tccggagtcc tggactttac ggatcgtttc caggacctgg tcctgggtga
     7381 cggactccgg cagccccagc tgctttgcga tacgcttcaa gaattccatc tcgacttcct
     7441 tttcagtagg gttggcgttt tttgcgacaa tgggctttat ccagttcaga cgcggggcgt
     7501 tggtaagggc tacccggaga agttcgacca ggcggcgctc gcccttcgat accaggaaca
     7561 caggggagaa gtagcggtac tcttttttag cgagcaggtc ggcggcggct tcggtccatt
     7621 cgacctttgc ccacaggccg tcctccccgc gattctcgat cgatttgatc catcctgcgg
     7681 cgggggcctt gccgtccggc gatgaatact ctccgccctc ggtctggtgt tcgtagtcga
     7741 tcaccatgtc gaggccgcgg gagtcgaatg cggagatcac ggccgacatg gctgcgtcgt
     7801 cgacgatgaa gggctcgccg tcgacaaggg tcacccggcc gtgcgggaag atctggaact
     7861 cggtggggac ggcggtcggc cgccccttat cttccggcag tccggcgatg aatccgagtg
     7921 tgtccgtcat tgacaaatcc ttgcaattgc actatttcag agattgcagg cgccggtttg
     7981 gtcgcggcct tacaccggct ccgggcgctg caggcgccgc gacgcctgac ggccccacta
     8041 ccttccgaat atcagtagcc cgcgcctttg actttccagg tacttccagc ccttggacgt
     8101 gaaaaaagtc caggcttcga gcatcccacg gaccgtctgc gccgacatga aaaggccccg
     8161 tcctttctca agctgaatcg ccttcaaaaa tctggctctc agtacgactt tcccggtccc
     8221 tttgtgtttt tcgaaactca gccaaacctc gtagggctcc tggattaatt cggggaaaaa
     8281 gggcaggaac gcagaccggt cgagcggaat atgctcggac agagacctcg cattaaccag
     8341 gaccggcatc ttcaaatccg cacctgccat gaaaatcttt tcatctccgc ccaggatccc
     8401 ttgcaaatac ttctcagcct cagccttcga ttgcatgggc gagactgccg aggtctgcgt
     8461 aatatcgaga gggagctccc ttggcctgcc ataagagagc cggtctccgg aggtcatccg
     8521 ctcccatgcg gccgctcccc ggcgtctcca ggcatccatc acctcttcgg ggagctgact
     8581 tccccaggcc gtttcaccca cgttgtacgc ccagcccgga tcgatgccgt tcggcacgcg
     8641 gaccgtccgt gtcgtcccgt ccgatagcct gatctccttt tcctgccagt ccatctccgg
     8701 ggcttcttcc ctgagttttt ccccttcggc cttcatccgc tccacttcag ggccggagtg
     8761 gttgaccacg gtgcaccggc acccccatcc gttgggaggg taatgggtac gccaccacgg
     8821 atcgtcatgt cggagcaccg tgttgtgcca cgcgacatgc tgcgtccgcg tgcggccgtc
     8881 catgaccgcc gaataacgcc agtaggggcg ggcttccaga acgtccgggt cggtcatctg
     8941 cttgtagtgc ccagcctgat aggcagtacg catgttggtg ttaaagatca ccgaagatct
     9001 ccatccacgc ccgcccttgt agctccagcc gtgccgcttc actatgctgt cgaagtcttt
     9061 tcgaaattcg ttgagagtga tccccttctc gatgcccata agaacggcgg cctgaaaatc
     9121 ggagagcagg tcgtctttca tggcgccggc caccacgaat gcccggctgt gcatgccctc
     9181 ccagatatcg gtccaggtcc gggtcgggag cctgaccttc tgccggaaaa agtcgatcgc
     9241 ctcctggaag ggcagtccct gggctaggat ctctgtagat gggtagtcca tacccttccc
     9301 ccctcggcgt cgaagcgccc ggacagatcc gccagggcga aggcctgctg catgatctct
     9361 ccaagccgcg tcgggtcaat atcggcatat agattgatca gccggtcgcg gacctcttcg
     9421 agcgaggcgg ccgaatccac aagatcccgg atccggtctg tcatgccggt catttcgagc
     9481 atggcacggt cctggagctg atcggcgata atgtcggcag cgtcccggtt cgaggcggca
     9541 agctttgcga tgagctttgc gatgtcttcg gcggcgtcac ccggctgctt cccgcccggc
     9601 tcgtttttcg gcgacggccc ggccgctcgc cgcggagcga gcggcgtctc gcccggttgc
     9661 ggaagcggga tcttaaagcg cgcggacagg tgctcctggg ataccggctg gcccatgtcg
     9721 gcgcagagga tcttgtagat ggcggcgtag gcctcgaggt cttcaggagg ctcgtagagg
     9781 aagcgaaagc acgggacagg cgcatcccag ccgtagttga agcccaccag ggggcgaagg
     9841 atctgatggg tgatggtctt tgcaagcgcc cggcagtcgg cttctacgag atcctggcgc
     9901 acatctccgt ggacgcggcc gagggcgaga gatccccggc ccttttcacc gccggcttcg
     9961 gaggtgagcg tctggccaag aatcgccttg gacatctgag cgtcgcagaa gcgcgcgagt
    10021 ccctcgtaaa tgagctggcc ggaagatccg gaacccttct gggtctcgat gaactcgatt
    10081 tcggtcgcct ttgaaatgat tccggctgcg tcggagccca gcatgcggac ggcggacagc
    10141 aaagcgtcct tgtcggcctt gccggcgccc ggttcgtatt taccgacgcg gatcggcatg
    10201 ccgtagactt cggagaacga cacccagtcc ttgacggcgt aatttttgaa cagatacatc
    10261 caggcgcaga cgcgcatgat gcctgccctg gtgtcgtagc cggatcgggc cttgtatcta
    10321 tggtaggcga acttgtaggg aggcggatcg atcccacgga tcggttcatc atcggtcagc
    10381 acgcgcgggt agaccgaatc tcggaaggtg atcttcttgg gatggacgta gcggagaccc
    10441 cggatcgccg cctggccggc tgatacgtcc caaaggattt ccatcatcga gtagcccttt
    10501 gaaatggcgt ccaggaggtc caatatatgg tcgtcgaaat ctgggaggcc gtcgatcgcg
    10561 aaattgcaaa attctgcaat ctctttggat gtggcgtctt cggctgccgg gagtatttcc
    10621 caggaaaggc cctggacgga gagcttgcgg gtctgcagct ggctggcgag atgggcgtct
    10681 ttttcttcca tttcctcgaa cagctccgcc tggcgcacca cgtcgccgtt gtcggcctcc
    10741 ttgaggatgc gggccaggcg ctgcggggtc agcccgtagc tgggatagga ggaccacctg
    10801 tcgcggacct ggacgacgga aatatcgcgg gtttcggggg acctgatgtc gcgttcctgg
    10861 atgggcctgc cgaattgatc gtaaagcatc agtagcaccc cctctgctct gcgaagcggc
    10921 gcccgctgga ggattcgaat tctatgggtc ccgtcggctt gccggcggcg ttggctgcca
    10981 gaatatactt tcccccaaat tttggacagc ctgttaagct tcgatgcagg tcgaaac
//
LOCUS       DAOOVC010000128        11015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3849_length_11015_cov_6.791971, whole genome shotgun sequence.
ACCESSION   DAOOVC010000128 DAOOVC010000000
VERSION     DAOOVC010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_11015_cov_6.791971"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1331
                     /locus_tag="PLM79_13945"
     CDS             165..1331
                     /locus_tag="PLM79_13945"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HOV87462.1"
                     /translation="MTERERTCDKLLLLVGSNPLPNYITALILKPKSIRLFYSPETEK
                     VKKHLWEALTTGLSGAQLEETCIDDVTSAAKVRQAFGSIPSDAHLHYTGGTKIMAAHA
                     RMAFRESGGKDEQASYLDERKGVLRFDDRYEMDLSKQRLRLTLGDVLALHAIKRISED
                     HLQPDGPAGEDAGIIAGAVLNDPSLAKRLYGIHRDEKNKRRSFEKAKADAVILDELVP
                     GDLSVRRIPEAHWKRQAYENWLDFLGGGWLETWCSALVRQIVDGDEIAVGLECKPANG
                     RQCEIDVALVRGHRLYVISCTTDTKTGLCKSKLFEVAMRARQLGGDLARSALVCLLHG
                     SDAKGAFVDQLRNDVADVWDAPNTPQVFGLDDLKEWAGICGRPNMDSLRRWLDS"
     gene            1598..2920
                     /locus_tag="PLM79_13950"
     CDS             1598..2920
                     /locus_tag="PLM79_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87463.1"
                     /translation="MEVALVHKEYDDGSLARTLLDIQIELARTKTERFPSAPLLGLSV
                     TASCKETGLPAIGFESSEPKISLSKEILKRRDKLEEANRHWEAYLKGWDEFAFPSELD
                     HLGRSIGDTSLIGLVHVDGNSVGSKIKKWLMGKSENGVDGDTVRREYLEWSQAIDCLG
                     KTALQAVVNRICQRLEKTVEDRTEKMKVTGKPARLAFELKQEDGRWMLPLRPILLGGD
                     DLTFVCDGRIALDLAETALNVFDTSDIPHLGKITACAGAAMVRVHAPFARAYDLSEKL
                     CASAKRMLKKSNGEGCALDWHIGISRPGETVEGIRERQYRTNGCWLTCRPYRLGSGKN
                     EAGTWKWLSGTLLDDKITGLNEGAWFRRRNKVKAFAELAREGPKSVHAALEAWKVVDR
                     NLQLPQPIAGNGFFAETRTPLIDALELLDLHLMLDAAGEHGAEEGKTL"
     gene            2917..3552
                     /locus_tag="PLM79_13955"
     CDS             2917..3552
                     /locus_tag="PLM79_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87464.1"
                     /translation="MNQNSFIQIELLSDATFSRGEGTAGVVDVEVEHDERGLPFLGGK
                     TIRGLLRDSWLSMHHCFPELAQTTGRVFGPEADCDESSILRIGDAVLDEETRRWIETA
                     ESRPSHSISPAAVLEALTDIRRQTSEDRSTGAPAETTLRAVRVIVRGLTLRAPLHWLA
                     DPTPQDVRCLSLALLAARHAGLSRNRGRGHIRMTLDGDLEKTRNAAKGGSA"
     gene            3549..5225
                     /locus_tag="PLM79_13960"
     CDS             3549..5225
                     /locus_tag="PLM79_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87465.1"
                     /translation="MTFHRYLPYILTLQSPAIITALGGDPNSSSTLPFIPGAAVRGAV
                     AKALGDPGRDTARQKEFRDLIFGGKVGYLNAYPSLGGLRSVPVPVSLRRKKDDPGGNH
                     QVKVVDLTAFDGHPTRDDEPGECWPEEQLAPLGQEFLTIGAAQPVLLQPKINARIHHR
                     RDREKGRAWKDRDGTPHGAIFAFESIDAGQSFQGMIQVWGATEEELDPTENRIKDLLG
                     QAILIGRSRRAGYGGMAALQWGEGQEREVYGPGREGLRPVAGDISKDTVFRLLLTSAC
                     IVRDGSTGQINPAALPELIERRFGRRAKLVRTRWAFSPIGGFNRKWRLELPQVLAVSA
                     GSVFLLQADHNIPVGDLYDIENEGLGERREEGYGRVLFLDAPLPEFSLREPEETAPVS
                     AGDGQPPHVVQDIEKRIVLAQLARKIEQKAADLAGSAGNLPSNSLIGRLRTLLRGEPE
                     EAIETLKRWLRDGSEAERLKRSAMDQLERCRMDGNKHLADWILEATDRDRVLSWLNAE
                     VLAQRCHIISEESAKGILKEKPEEISVKLIDAVLAALAVRNKTEEAGDER"
     gene            5215..6726
                     /locus_tag="PLM79_13965"
     CDS             5215..6726
                     /locus_tag="PLM79_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOV87466.1"
                     /translation="MNANSWQLSNGGGIRPMAARWVITGEMRLETAAHLGGEGDAAVD
                     MAVLRDPKEGVPLLPGTSLAGALRSHLADVLGGYRSSEQQEVEELFGTARSPKRQEPT
                     APFEGAPSDDAGSQSLLIVFDSLGTLPDGAAPEIRDGVAIDPATGTAEAHKKFDFEIL
                     PAGTCFPVRVELIVGDLKKEAQLLVLLVTALNGLGEGDIGLGMRRSRGLGAISVHDWK
                     AVRHDLATPQGWLCWLTSDHEKPIGDHVPGHGSAAEAIRAAFPNLKIEKFADRRRRLL
                     VDLKLDLAGDLLVRSPAADAGAPDVVHLHSAGKPVLPGTGLAGALRAQALRIARLVRE
                     SQGDWDKFVDRLFGPRLEREDANSQGGQVMASRLRISESFIENGEGRRQTRIAIDRFT
                     GGVVRGALFDEQVLAGGNLKVRLELRDPADAETGLLLLLLKDLLSGDIPVGGAVSVGR
                     GVLRGTARVRLPDGAAYEIAKDLSVNEEARKVFDKKIAAFHEAAPFNAEEARS"
     gene            6723..7319
                     /gene="csx19"
                     /locus_tag="PLM79_13970"
     CDS             6723..7319
                     /gene="csx19"
                     /locus_tag="PLM79_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx19"
                     /protein_id="HOV87467.1"
                     /translation="MNRIRGCTIEPLDRQTCLAVLAWVTGGTAPAGCDHGFTWLLAHC
                     RDGVTWGRRENSRWRLSSEPFPDLCPAVSRDNLLETRLFGEKGEIMLWRSDEEFLGRR
                     LVDEPYQPMDWPTRPEEETRILMGYRLLEGPKQGFSRVTTAEGTQHAVPLECTAKDFT
                     GGRWPLRLRVRHYFEQDPQTGAVRVAASRLVHVFKEVR"
     gene            7319..9571
                     /locus_tag="PLM79_13975"
     CDS             7319..9571
                     /locus_tag="PLM79_13975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOV87468.1"
                     /translation="MGRHVNPTVPARSATAPYNFVPLPNRVFVVENGIEVNGKKIRPW
                     KMHDQFVYGSRTGWIDLEITTLTPLFIRGGIIEKGGVWDKRDARLRPEPFTSSNGRPM
                     IPGSSLRGMLRTLVEILSFSKIKLVTDEKPFFRTVAADRIGIAYRNRMVRGNNKPIGG
                     YLRRRGNEWTIVPAAEVLRVHRDELNNQLNLEIPAKPAPKYFPDWKCQHTTCWFQRDI
                     QKNWRVKKISPVRKGDWEEGTLVLTGSAPKKKYDFVFVGEDTGKSISIPEVLWRRFHD
                     DDQLTQWQEKAFPKDKPSEGCRKSKGYLRDGEPVFFLADDSAKTDENPAGLVFFGRAQ
                     MFRFPYDLSPLDLLPVEVRNAGLDMAEALFGMVGQTKNKKDQAIKGRVFFEDAIATSD
                     GPELFEEIIVPRILASPKVTCFQHYLTQDGSKGQKDLTTYLNDDHTTVRGHKLYWHKW
                     ADQGFEAVKESNSHSELLNDLQSGNPNDTQHTVIRPVKAGVVFKGRIRFDNLADMELG
                     ALLSALALQEGCVHRLGMGKALGLGSIKIEWKLHLADRAARYRCWENPGVTEKDGGEF
                     ITAFEKAMIEHARNTWEPIDENMSGLKRIGRLQAMFHLLQWTGKPPFSATEYMELQKF
                     KSRPVLPTPHKVIGQEEPKWKSDPPRPSQEDTASAPTGGTKPDPAIALTPPPPQVAKP
                     VEKGQIRTGKLKQKSGQWVALFEGDHREAVIVNQNRIDPGTAEGCVAEFYITEQSKKA
                     GIKARFEKFV"
     gene            9626..10651
                     /locus_tag="PLM79_13980"
     CDS             9626..10651
                     /locus_tag="PLM79_13980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HOV87469.1"
                     /translation="MTNRDGRPWGGWNLRNGDPLPADDEVVRWLRRADAAAASAETNT
                     LARLDLAETDILVLLHSDTLEGRFCAERLRTYYASSVRETILVHIGKLGYGAAQFTSG
                     LKALVDAAIRRVHQAQDRGIQTVFCATGGFKAEIAFLNLMGALLGIEVVYIHELHREL
                     VRLPRLPLTWDAAFVARHDDFFRWIDSEPRKSAEVESWLKGRPELRPLVEDGGDGHTY
                     LSAAGDLLYKVAAERLSMGPRALWPEADPRPPHEKNHLSGVEHHRATGWERFVDRLCA
                     IDCVTSVHYDSRCAGIRSVKVMDAANGVIGVCYGGPEDVLPLRVETTARGEAQTDLVA
                     SYLQHMK"
BASE COUNT         2429 a         3044 c         3382 g         2160 t
ORIGIN      
        1 acggccaggt gagccagctt gtccgcaagg gctatctgaa gcgcgaaccc cggaaagctc
       61 gtggcattgc gattgtccgc gaacccgagg cctcttttgg ttcgctgcct gaagaagcgg
      121 agatgacaat gccgtccaac gataagccag tgggagggtg acgaatgact gaaagagaaa
      181 ggacatgtga taaacttctc ctgctcgtcg gttccaaccc tttgcccaat tacattaccg
      241 ccctcattct gaaaccaaaa tccatccgcc ttttctacag cccggaaacg gaaaaagtga
      301 agaagcacct ttgggaagcg ctgacgaccg gcctttcggg tgcacaactg gaagaaacat
      361 gcatcgatga tgtaaccagc gcggccaaag tcaggcaggc gtttggctca attccttccg
      421 atgctcacct tcactacacc gggggcacaa agatcatggc agctcatgct cggatggcct
      481 tccgggaatc cggcggcaaa gatgaacagg cttcctacct ggatgagcgg aaaggggtgt
      541 tgcgtttcga cgatcgctat gaaatggatc tgtcaaaaca acgtctccga ttgaccttag
      601 gcgacgttct tgcgcttcac gcaatcaagc gtatcagtga ggaccaccta cagccggatg
      661 gccctgccgg cgaggacgcc gggatcatcg ctggagcggt cttgaatgac ccaagcctgg
      721 caaaaagact ctatggcatc catcgtgacg aaaagaataa acgtcggtct tttgaaaaag
      781 caaaggctga tgccgtaata cttgacgaac tggtgcccgg cgatctgagc gttcggcgga
      841 ttccggaggc tcattggaag aggcaagcct acgagaattg gttggatttt ctggggggcg
      901 gatggctgga gacctggtgc agcgccctgg tgcggcagat tgtcgatgga gacgagattg
      961 ccgtcggcct cgaatgtaag ccggccaacg ggaggcagtg cgaaatcgat gtggcgctcg
     1021 tccgaggcca tcgactctat gtaatctcct gcaccacgga caccaagacc ggcctctgca
     1081 agtcgaagct tttcgaggtg gctatgcggg ctcgtcagtt gggcggggac ctggcccgtt
     1141 cggccctggt gtgccttctc cacggcagcg atgccaaggg cgctttcgtg gatcaattgc
     1201 ggaacgatgt ggccgacgtc tgggacgcgc ccaacacgcc gcaggttttc ggtctcgatg
     1261 acttgaagga atgggcggga atctgcggcc ggccgaatat ggactcactc agaaggtggc
     1321 tcgattcata ggggggatta tgcctgtatt gacagcgatc gatgtgctgg gggtacaacg
     1381 gttcgtcttt ctttcaaacc ggctccggga cgtagtgacc ggctcgtacc tggtccactg
     1441 gagcacctct gcggagggtg cgctcaaggg cttggtcccg acggataaga tccttttggc
     1501 cggcggggga aacgcgattg tcgaaaccgg gtccatgcag gatgcacgct cgcttgcagc
     1561 ccgatatatc cgccgattgc atgacgaggc cccgggtttg gaagtcgcct tggttcataa
     1621 ggagtacgac gacggaagct tggcccggac gcttctcgac attcagatcg aacttgcgcg
     1681 cacaaagacc gaacgcttcc cgtcggcacc tcttctgggc ctttccgtca cggcttcttg
     1741 caaggaaacg ggtcttcctg ctatcggttt cgaatcaagt gaaccgaaga tttccctttc
     1801 caaagaaata ctgaaacgcc gggacaagtt ggaggaagcc aatcggcatt gggaagcgta
     1861 tttgaaaggt tgggacgaat tcgcatttcc gtcggaactg gatcatttgg gtcgaagcat
     1921 tggcgatacc agcttgatcg gcttggttca cgtggatgga aacagcgtcg gcagcaaaat
     1981 caagaaatgg ttgatgggaa aatccgagaa cggcgtggat ggcgacaccg tgcgtcggga
     2041 atacctggag tggtctcagg ccattgactg tcttggaaag acagccttgc aggctgtggt
     2101 caatcgtatc tgccaacgac tcgagaaaac tgttgaagat cgcacggaaa agatgaaggt
     2161 caccggtaaa ccggcgcgtc tggcctttga actgaaacag gaagatggcc ggtggatgct
     2221 tcccctgcga ccgattctgc ttggggggga cgatctgacg tttgtctgcg acggccgcat
     2281 cgcattagac ttggctgaaa cggccctcaa tgtcttcgac acaagcgaca tccctcatct
     2341 aggcaagatc accgcctgcg ccggggcggc catggtccgc gtgcatgcac cctttgccag
     2401 ggcatacgat ttgtcggaaa aactgtgcgc ttccgccaaa cgcatgctga agaagagcaa
     2461 tggggagggc tgcgcgctgg attggcacat cggtatctcc cggccggggg aaacggtgga
     2521 aggcattcgg gagaggcaat atcgaacgaa cggctgctgg ttgacctgcc gcccctatcg
     2581 gctgggctcc gggaaaaacg aggcaggaac ctggaagtgg ctcagcggca cgctcctcga
     2641 cgacaagata acgggcctga acgaaggagc ctggttcagg cggcgcaaca aggtcaaggc
     2701 ctttgccgag ctggcgaggg aaggcccgaa gagcgtgcat gcagccttgg aagcctggaa
     2761 ggtggtggac aggaatcttc agcttccgca gcccatcgca ggaaacggtt tcttcgccga
     2821 gacacgcacg ccgctcatcg acgccctgga gttgctcgat cttcatctga tgctggatgc
     2881 ggctggagaa cacggggcag aggagggaaa gacgctgtga accaaaattc attcattcag
     2941 atcgagcttc tctccgacgc aactttcagc cggggagaag ggacggccgg tgtggtggac
     3001 gtggaggtcg agcacgacga acgggggctg ccgtttctgg gaggcaagac catccgcgga
     3061 ctccttcgcg attcctggct ttccatgcat cactgttttc ctgagctagc gcaaacgacc
     3121 gggcgggtat tcggccccga agccgattgt gacgagtcct ccattttgcg catcggcgac
     3181 gccgtgctgg acgaagaaac ccgccgatgg atcgaaacgg cggaatccag gccaagccac
     3241 tccatttccc cggcagccgt gctcgaggca ttgaccgata tccggcggca gacctcggag
     3301 gacaggagca cgggagcacc ggccgagacc accctgcgcg cggtacgggt gatcgtccgg
     3361 ggactgacgc ttcgagcgcc cctccattgg ttggccgatc cgacccccca ggatgtccgc
     3421 tgcctctctt tggcccttct ggcggcgcgc cacgcggggc tgagccgcaa ccgcggacgg
     3481 gggcatatca ggatgaccct cgatggagac ctggagaaaa cccggaacgc agcgaaagga
     3541 ggatcagcat gactttccac cggtatcttc cctatatttt gacgctgcaa tccccggcca
     3601 tcatcaccgc gcttggaggc gatcccaaca gctcctcgac tctgcccttc attcccgggg
     3661 cggccgtgcg cggggccgtg gccaaggccc tgggcgatcc gggcagggat acagccaggc
     3721 agaaagaatt ccgggatctc attttcggtg gaaaggtagg gtatctgaat gcctatccgt
     3781 ctctgggcgg cctcagatcc gtcccagtcc cggtatctct acgtcggaaa aaggacgatc
     3841 cgggaggcaa tcatcaggta aaagtcgtcg atctcacggc gtttgacgga catcccacac
     3901 gggacgacga gccgggtgaa tgctggccgg aggaacaatt ggcgccgctt ggccaagaat
     3961 ttctcaccat cggcgcggcc cagccggtgc tccttcagcc gaagataaac gcccgcatcc
     4021 atcaccggcg cgaccgcgaa aaaggccggg cgtggaaaga ccgggacggc actccccacg
     4081 gagcgatctt cgccttcgag tccattgatg ccgggcagtc ctttcagggg atgatccagg
     4141 tgtggggcgc aaccgaggaa gaactggatc cgaccgaaaa ccggatcaaa gatttgctgg
     4201 ggcaggccat cctcatcggc cgctcccgaa gggcgggata cggcggcatg gcggcgcttc
     4261 agtggggtga aggccaagag cgtgaggtgt acggaccggg aagagaagga ttgcggcccg
     4321 tggccggcga tatttcaaag gatacggttt tccgcctgct tcttacgtcg gcttgcatcg
     4381 tgcgcgatgg gagcaccggg caaatcaacc cggcggcatt gccggagttg atcgaaaggc
     4441 gattcggccg ccgggcgaag ttggtgcgga caagatgggc cttttcgccc atcggcggtt
     4501 tcaatcgcaa atggcgcctg gaactgccgc aagtcctggc cgtttcggcc ggttcggtgt
     4561 tcctgctcca agccgatcac aacatccctg tcggcgacct ctacgacatc gaaaacgagg
     4621 gattgggcga acgaagggaa gaaggctacg gacgggtcct gtttctcgat gcgccgctgc
     4681 cggagttttc cttgcgcgaa cccgaagaga cggctcctgt gtcggcgggc gacggtcaac
     4741 cgccccatgt tgtccaggac attgaaaaga gaattgtcct cgcgcaactc gcaagaaaga
     4801 tcgaacagaa agcagccgac ttggccgggt ctgcagggaa ccttccaagt aacagcctga
     4861 tcggccggct acggactctc ttgcggggtg aacctgagga ggcgatcgag accttaaaac
     4921 ggtggttgcg ggatggaagc gaggcggagc ggttgaagcg gtccgccatg gaccagctcg
     4981 agcgctgccg gatggatggg aataaacact tggctgactg gatcctggag gcaactgatc
     5041 gcgacagggt tctctcgtgg ctcaatgcag aagtcctggc tcagcgctgc cacatcattt
     5101 cggaagaatc ggccaagggg atcttgaaag aaaaacccga agaaatctcg gtcaagctca
     5161 tcgacgcggt tctggccgcg ttggccgtcc gcaacaagac agaggaggcc ggtgatgaac
     5221 gctaattcgt ggcaattgag caatggcggc ggcatccgtc ccatggcggc ccgatgggtc
     5281 ataaccggcg agatgcggct cgagacggcc gcacacctgg gaggcgaagg cgacgccgcg
     5341 gtggacatgg ccgtcctgcg cgatcccaag gagggtgtgc ccttgcttcc cggcacctcg
     5401 ctggccgggg cgttgcgcag ccatctggcc gatgtcctcg gcggataccg ctcctcggaa
     5461 cagcaggaag tggaggagct tttcggaacg gcccggagcc cgaaacgtca ggaaccgacg
     5521 gctccttttg agggagcccc gagcgacgac gcaggctctc aaagtctttt gatcgtcttc
     5581 gattctttgg gaaccttgcc cgatggggcg gcgccggaaa tccgcgacgg cgtggctatc
     5641 gatcctgcca ccggcacggc cgaggcccac aagaagttcg acttcgagat tctgccggcc
     5701 ggaacgtgtt ttcctgtgcg tgtagaattg attgtcgggg atctcaagaa ggaagcgcaa
     5761 ctcctcgtcc ttctcgtaac cgctctgaac ggtcttgggg aaggcgacat cggccttggg
     5821 atgcgacgct cgcgagggct gggagctatc tcggtccatg actggaaggc cgtccgccat
     5881 gatctcgcca cgccccaggg ttggctctgc tggctgacat ccgatcatga aaaacccatc
     5941 ggggaccatg tgccgggtca cggctcggcc gccgaggcca ttcgagctgc ctttccgaac
     6001 ctgaagatcg agaaattcgc agacagacgc cgccgcctgc ttgtggatct caagctggac
     6061 ctggccggcg accttttggt ccgcagccct gcagccgacg caggtgcacc tgacgttgtc
     6121 catctacaca gcgcgggaaa acccgtgctc cccggaaccg gcctggccgg ggcattgcgc
     6181 gcccaggcgc ttcgaatcgc ccgtctcgta cgggaaagcc aaggtgattg ggataaattc
     6241 gttgaccgcc tttttggccc ccgactcgaa agggaagacg cgaactccca aggtgggcag
     6301 gtcatggcct ccaggctacg gatctccgag tcctttatag aaaacggcga gggccggagg
     6361 cagacgcgga ttgccattga ccgctttacc ggaggggtcg ttcgaggcgc gcttttcgat
     6421 gagcaggtgc tggcgggcgg caacctcaag gtgcgactcg agctgcgcga tccggccgat
     6481 gccgaaaccg gcttgctcct gcttttgctc aaggacctgc tttcgggaga catcccggtc
     6541 ggcggtgcgg tatcggtggg ccggggagtg ctccggggaa cggcccgggt tcggctgccg
     6601 gatggggcgg catacgaaat cgcaaaggac ctttcggtga acgaagaagc ccggaaggtc
     6661 ttcgataaga agattgcggc ctttcatgag gctgctcctt ttaatgcgga ggaggcgcga
     6721 tcatgaaccg gatccgagga tgcaccatcg aaccgctgga tcggcaaacc tgcctggcgg
     6781 ttcttgcgtg ggtgaccgga ggaacggcgc ctgccggctg cgaccatggt ttcacctggc
     6841 ttttagccca ctgccgggac ggcgtgacct gggggcgacg cgagaattcc cgctggcggt
     6901 tatcctcaga gccctttccg gatctctgcc cggctgtttc cagggataat ctcctcgaaa
     6961 cgcgtctttt cggagagaaa ggggagatca tgctctggcg gagcgatgag gaattcctgg
     7021 gtcgtcgcct ggtcgatgag ccctaccaac ccatggactg gccgacccgg cccgaagagg
     7081 agacccgcat cctgatgggg taccgtctcc tggagggacc caaacagggt ttttcccgcg
     7141 tgactacggc cgagggaacc caacacgcgg ttccactgga gtgcacagca aaggatttca
     7201 cgggcggtcg ctggcccctt cgactcaggg tgcggcatta ctttgaacag gacccgcaga
     7261 ccggggcggt ccgtgtcgcc gcaagccgtc ttgtccatgt cttcaaggag gtgcgctgat
     7321 gggccgacat gtaaacccga ctgttcccgc aagatccgca accgcccctt acaattttgt
     7381 tcctttgccg aacagggttt tcgttgtgga gaatggaatc gaggttaatg ggaaaaagat
     7441 acgtccctgg aaaatgcacg accagttcgt gtatgggtcc cgaaccgggt ggattgattt
     7501 agaaataacg actctgacgc ctctgttcat ccgaggaggg ataattgaaa agggcggggt
     7561 gtgggacaaa cgcgatgccc gactgcggcc tgaacccttc acatcctcaa atggcagacc
     7621 gatgattcct ggctcaagcc tgcgcggcat gctccgcact ttggtcgaaa tcctttcctt
     7681 tagcaagatc aaactggtga cagatgaaaa accatttttc cgcaccgttg cggctgatcg
     7741 gataggcatc gcctaccgaa atcggatggt tcgcggaaac aataagccga tcggtggtta
     7801 cctcagaaga agagggaatg aatggaccat tgtccccgcg gcagaggttt tgagggtcca
     7861 tcgagatgaa ttaaacaatc agttaaactt ggagatccca gccaagccgg ctccgaaata
     7921 cttccctgac tggaagtgcc agcacactac ctgctggttc caacgcgaca tccagaaaaa
     7981 ctggagggtc aagaagattt ctcctgttag gaaaggcgac tgggaggaag ggacattggt
     8041 gctaaccggt agcgcgccaa agaaaaagta cgattttgtc tttgtcggag aggacacagg
     8101 caaaagcatc tccattcctg aggtgctgtg gcgccgcttt cacgacgatg atcagctcac
     8161 ccaatggcag gaaaaggcgt ttccgaaaga caaaccctcg gagggttgtc gcaagtccaa
     8221 aggatacctg cgggatgggg agccggtgtt ttttctcgcc gacgattctg ccaagaccga
     8281 cgaaaacccg gcaggactcg tatttttcgg gcgggctcag atgtttcgtt tcccctatga
     8341 cctgagcccg ttggacttgc tgccagtcga ggtgagaaat gccggtttgg atatggccga
     8401 ggctttgttc ggtatggttg gccagaccaa aaacaaaaaa gatcaagcca tcaaggggcg
     8461 tgtctttttt gaggatgcca tagcgaccag cgacggccca gaattgttcg aggagatcat
     8521 tgttccgaga atactcgcct cgccaaaagt tacttgcttt cagcactatc tgacacaaga
     8581 tggttcaaag ggccaaaagg atcttaccac ctatttgaac gacgaccata caacagtccg
     8641 aggtcataaa ctttattggc acaagtgggc cgaccaaggg ttcgaggctg taaaggaatc
     8701 caatagtcac agtgaactgc tcaatgattt gcagtcaggc aatccaaacg acacccaaca
     8761 tacggttatc cgacccgtca aggccggcgt ggtctttaaa ggtagaatcc gttttgataa
     8821 cctagccgat atggagttgg gggcgttgtt gtccgccctc gctcttcagg agggctgtgt
     8881 tcatagattg ggcatgggca aagcgttggg cctcggaagt atcaaaattg agtggaaact
     8941 tcaccttgct gaccgggccg ctcggtatag gtgttgggag aatccggggg ttaccgaaaa
     9001 agacggtggt gaattcatca cggcttttga gaaggcgatg attgaacatg ctcgaaacac
     9061 ctgggagccg atcgatgaga acatgtctgg actgaaaaga atcggcagac tccaggccat
     9121 gtttcatctt ttgcaatgga ctggaaaacc tccgttttcc gcaacggaat atatggaatt
     9181 gcaaaagttc aagtccaggc cggttctacc tacaccgcac aaagtcatcg ggcaagagga
     9241 gcccaaatgg aaaagcgatc cgccgcgtcc atcccaagag gatacagctt cagccccaac
     9301 gggaggtacc aaaccagacc ccgcaatagc actgacccca ccgccccctc aagtcgccaa
     9361 acctgttgaa aaaggccaaa tccgaaccgg taagttgaaa caaaaaagtg ggcagtgggt
     9421 tgccttgttc gaaggtgacc accgcgaagc ggttatcgtc aaccaaaaca gaattgatcc
     9481 tgggaccgcg gaaggctgcg tagcggagtt ctatatcacc gagcaaagca aaaaggccgg
     9541 gatcaaggcc cgctttgaga aatttgtgta aactggagat caattgatgc cccgccaaat
     9601 cctttgcacc gtaggaacat cgctattgac caaccgcgac ggccgcccct ggggcggttg
     9661 gaatctgcgc aacggtgatc cgttgccggc cgacgatgaa gtggtccgct ggctgagaag
     9721 ggccgatgcg gctgcggcca gcgctgagac caacaccctg gccaggctcg atcttgccga
     9781 aacggacatc ctcgtgttgt tgcactccga cactctcgag ggccgctttt gcgcggagcg
     9841 actcaggacc tactatgcct cgagtgttcg ggaaacgatc ctggttcaca tcggcaagct
     9901 gggttatggg gccgcccagt tcacctcggg actcaaagct cttgtagacg cggcgatccg
     9961 ccgggtccat caggcccagg accgtggcat tcaaacggtc ttttgcgcca ccggcggatt
    10021 caaggcggaa atcgccttcc tgaacctcat gggggcgctt cttggaatcg aagtcgtcta
    10081 cattcacgaa ctgcacaggg aattggtccg gctgccgcgt cttccgctca cctgggatgc
    10141 ggcattcgtc gcaaggcacg atgacttttt tcgctggatc gattcggagc cgaggaaaag
    10201 cgccgaagtg gaatcgtggc tgaaaggaag gcccgagctc aggcccctgg tggaagacgg
    10261 cggagacgga catacctacc tttcggcggc cggggacctt ctttacaagg tggccgcgga
    10321 gcgtctctcc atgggaccgc gggcgctctg gcccgaagca gacccaagac caccccatga
    10381 gaagaaccac ctttcagggg tggaacacca tcgggcgacc ggatgggaac ggttcgtgga
    10441 taggctttgt gccatcgact gcgtcacttc ggtccattac gattcacgat gtgcgggaat
    10501 ccgtagcgtg aaagtgatgg atgcggcaaa cggcgtcatc ggcgtttgct atggtggtcc
    10561 ggaagatgtg ctgcctcttc gggtcgagac cacggcccgc ggcgaggcgc agaccgatct
    10621 tgtcgccagt tatctccagc atatgaaata atttgccttt tccacaaggc gcgtgtcgct
    10681 tataacccac cagccctttc ccccaacctg cgaaaccccg tgaacggcgt cgaataacac
    10741 caccgcccgc catgcagcga cccgcgaaac acgcggactt gcggccatcc cgggcctggg
    10801 aatgtcggat aataccaagt tgcgcccaag atcgccctga catgctgagt gaagatttcg
    10861 agcaaaggga tgaatcctgc gggtgggcag ttccggcttg aatgcaactt ggtataagac
    10921 gtgttctctg accttccgag aacctcggtc ggcggaaggc tctctcgatc aatgcttccg
    10981 cccggctccc gaaggctccg atgctgcccg catca
//
LOCUS       DAOOVC010000129        10983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3863_length_10983_cov_6.501373, whole genome shotgun sequence.
ACCESSION   DAOOVC010000129 DAOOVC010000000
VERSION     DAOOVC010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10983
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3863_length_10983_cov_6.501373"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLM79_13985"
     CDS             complement(<1..502)
                     /locus_tag="PLM79_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87470.1"
                     /translation="MERKRVVIVGAVALGPKAACRLKRLRPEFEVTMIDQDEHISYGG
                     CGMPYFISGDVSDVRELMSTSFHMERTPKFFEDAKDIRVRTRTRATAIDRSNKTVHLV
                     DLATGKEEDLPYDHLVLATGSRPNSLPVPGADLPGVMSLGNLRSAIEIKDLITRGKVG
                     KAVIIGG"
     gene            complement(526..732)
                     /locus_tag="PLM79_13990"
     CDS             complement(526..732)
                     /locus_tag="PLM79_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87471.1"
                     /translation="MEPKRAKWRRPIQYRCATCDTDQPFCWTCPCGFMICSECMEENF
                     WGMTCNGINWTCPDCGAIRGFGND"
     gene            complement(750..2996)
                     /locus_tag="PLM79_13995"
     CDS             complement(750..2996)
                     /locus_tag="PLM79_13995"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOV87472.1"
                     /translation="MTDTIGSTAQQANMGSAREDDAVLAKLAVQHGFLTEQQVRDALS
                     FQREREESGTPPPLRQVLLDRGWISRRQLDALQLAADFLSLRKPDRLFGRLAVSRGLV
                     TREQADAASQMQARIYKETRVFRSLGDLLVEQGWLKAGVRDALLARMKSPELPSASSP
                     APPSKPAPAAPSAPAPTPPDATGSGPPGPASAQPKGRAQIRIESETLPDDTHILLEEE
                     SFDLLVSEDKLRAYLRIKASLSEGHGVELLTHALEAQGIVYGLLDPLELEALLQQPPS
                     RKQVWRIARGTDPQPGKDAEIRYCFPTRPKTLAPLEGEGKVDFKDLGEIPRVKKGDLL
                     AEKTPRVPETPGTDVFGNEIAVPKSKELTLLCGSGTEISPDRRRVTARVDGLPQLSLL
                     GRLSVLPELVIPGDVSFETGNIEYEGKITVHGVVQDGFRVKGSALIAREIGKAQIDVS
                     GDVVVYGGILGAKVRSQGNVNAVHIYDSQIEAMGNVVVDKGVVDSKISISGKLTCLRG
                     AVVSSRITARRGIEAAQVGLERSAPSVLVIGIDPLAEREIDRLKESLARHEAERAGHA
                     SLVRPLRERDAVIEARIGELAQVQDRTGLELRQVQSEIRELRERKDTAQLAQAEEYLK
                     EIEAVIGSAARELDALFEEQDEIRGKLARHKEQLRGMDQAIRDLRDELTALTEWNSAA
                     GRPPSVQVQGLVCSGTTVKGVFSSMVFKTDARFIQIREGRVGDTTSETPGKSSEMKLF
                     VTSLARSR"
     gene            complement(3303..4031)
                     /locus_tag="PLM79_14000"
     CDS             complement(3303..4031)
                     /locus_tag="PLM79_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HOV87473.1"
                     /translation="MKEYTIHPLTVGANETDQGIMTYQRGYGKRIWIPIYAFLILGGE
                     ERILVDTGLEQFVVPEEVSTRYGLEILEFESALEKHGLSPGDIDVILHTHLHNDHCEN
                     DYKCPQARIVVQKAEYEFFLDPHPIDHRYYPDLLDGLKVELVEGDTPFRDGIDLLLTP
                     GHTPGGQSIAVQTRNGLAIITGFCCNEMNFPAKGPAVAPGVHLDAVAAYESANRIRDM
                     ADILIPLHELSIGRHKVIPCPVSS"
     gene            complement(4128..4481)
                     /locus_tag="PLM79_14005"
     CDS             complement(4128..4481)
                     /locus_tag="PLM79_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOV87474.1"
                     /translation="MTFKSESFDPAGTLRNLDGDIELFQGLVLVFLEESASLLEEIRD
                     AIGLGDDARVQRAAHTLKGTSGNLGARGTMDAAFRLEVLARDGKVEDFPRAFEDLRAE
                     LDRLTRDLRRTLESP"
     gene            4554..8132
                     /locus_tag="PLM79_14010"
     CDS             4554..8132
                     /locus_tag="PLM79_14010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87475.1"
                     /translation="MTPLVSRPEKADNPTQLLTAIMESVTEGIITIDERGIIEMVNPA
                     AERMFGYSPGELVRKNIEVLVPEPHRGRHDEFLSRFLETGATKTPGPVRETEACRKDG
                     SVFPVSVAVSRFEIGGRLKVVGVFRDISERKRMLANLVESEARYRDMAILQAAVLDGA
                     NYSIIATFPDGIIRSFNAVAQRWLGYSAEEVVGKVTPEIFHDPVELARYAEELSKELG
                     RPIHPGFEVFAAKARQGSPVEREWTYVRKDGSRFPVLLSLTPLLDADGVITGFLGIAG
                     DITDRKRADEALARLASIVESSDDAIIGKSLDGVVLSWNKGAERLYGYRASEMLGRPI
                     SVLTPPGRSDELDGILDRIRRGERVEHFETLRIGKDGRAIDVSLTVSPITDPAGRICG
                     VSAIARDITGRKKAEEQLRRLSMAVEQSPVSVVITDITGRIEYVNPKFTEVTGYSSEE
                     AIGQNPRILKSGDKPPSEYKKLWETITAGGEWRGEFHNKKKNGELYWESASISPIKDD
                     KGNITHFIAVKEDITSLKEAREELAKLSLVASKTDNAVIITDAEGVIEWVNDGFVRLT
                     GYTLPEVVGRKPGSVLQGPLTDRLTVQRIREFLKARVPFTEEILNYHKEGFPYWVSMN
                     VTPIFDDSGRLIRFISIESDVTERRHAEEALRKAKEAADAASRAKTEFLSSMSHEIRT
                     PMNAIVGMAELLWESPLNDEQRKYVQMFRSAGDTLLNLINDILDLSKVESGQIELEAI
                     PFDLAEVIDRTCEVMAVRAHEKGLELACRIMPGLPTHLVGDPGRLRQVLTNLIGNALK
                     FTEQGEIVVEVKCRDLGGAGCTGDGGRQRDLLFSVRDTGIGIEPEKQEVIFEKFAQAD
                     TSITRKYGGTGLGLAITRKLVELMGGKIWVESTVGEGTTFSFSIPFEEAPAPVPKEPL
                     AEVDLAGVKVLVIDDNSTNRLILRETLSRWGAPVFAVGSGEEGIAELQKAKEAGNPYL
                     LVLLDQRMPEMDGFRVAERIKNLPDLAGTAVIMLTSDGMSLATGRSRELGIVSCLTKP
                     VKQSELREAINLTLGRFVPPAPRETPPAPEGLGTLNILLVEDSSANRFLIQAFLKNTG
                     CRIDTAENGQIAVEKFLSNRYDLVIMDMQMPVMDGYTATRTIRSIEKEEGLAPVPIVA
                     LTAHALKEDVQKSLDAGCDAHLNKPIKKAELIGAIMGYLKGPPPRP"
     gene            8610..8954
                     /locus_tag="PLM79_14015"
     CDS             8610..8954
                     /locus_tag="PLM79_14015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV87476.1"
                     /translation="MQLEGYKMGGVLVVRPLGRRIDASESPDFKGKMVDWINQGETRI
                     VLDLSGVDFIDSSGLGAVVSVLKTLGGEGELTIGGLREPVGSLFRLTRLDRVFRIYPS
                     AEDAVKAMSQVS"
     gene            9166..9879
                     /locus_tag="PLM79_14020"
     CDS             9166..9879
                     /locus_tag="PLM79_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87477.1"
                     /translation="MGRGLRNSQNLSFVGMLALTALVTVFAYQEIHREWVLFRKAETA
                     FSKKGFSEAVSLYEEAIAAGARTPTAYRRLGESCLALGRFREALETYEQLLRLYPEDL
                     SAMKNLAALYDQFDRPEDAIALYLRYESRSSLDVPSLLRLANLYQRKQDWESAGSLYR
                     KILDRDPGSVAGRMGLAELLSWTRRYEEAEALYRDVLKEDPGHRRARIQLARVLSWSG
                     RFEESIEEYRTALNPSSGK"
     gene            10035..10742
                     /locus_tag="PLM79_14025"
     CDS             10035..10742
                     /locus_tag="PLM79_14025"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87478.1"
                     /translation="MQSLDPGHGRYRSLPLVFLGLLLCFPPVGYGASREATVGGPGQV
                     GSPETGVAPIQPVQPAPESLQEEIPDWKARWELARLLSYVKRYEESLVEYGRVLEEKP
                     DLWEAKVETAQVLYWSGRQSEASRTLEEIPAGHLDERARLLMADLYVARKAYDKAEPI
                     FRAHLKSHPEDYRAAVKLADVLSWTRRYEEAILLYKEVLNARPDDIQIRRKYAYVLIW
                     AGRPSEAAAELRKTLKP"
     gene            10776..>10983
                     /locus_tag="PLM79_14030"
     CDS             10776..>10983
                     /locus_tag="PLM79_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87479.1"
                     /translation="MSSRISSLRRGLVFVIFALVSFSAFWFPAGAGAVGESRSDEDFP
                     GAISDFEARLTLARLLSYRGDTLDE"
BASE COUNT         2085 a         3323 c         3463 g         2112 t
ORIGIN      
        1 cccctccgat gatcaccgcc tttcccacct ttccccgggt gatgaggtcc ttgatttcga
       61 tggccgatcg caggttcccg agactcatca ctcccggcag gtcggctccc ggcaccggca
      121 gggagttggg acgacttcct gtggccagga ccaggtgatc gtaaggcaga tcctcttcct
      181 tgcccgtggc caggtccacg aggtgaaccg tcttgttgct ccgatcaatg gccgtagccc
      241 gggtgcgggt cctcacccgg atgtccttgg cgtcctcgaa aaacttcggc gtccgttcca
      301 tgtggaaact ggtgctcatg agctcccgaa cgtcgctgac atcccccgag atgaagtaag
      361 gcataccgca tccgccgtag gagatgtgct cgtcctggtc gatcatggtg acttcgaatt
      421 cgggcctgag ccgtttgagg cggcaagccg ccttgggacc cagcgcaacg gcgccgacaa
      481 tgaccacccg ctttctttcc atccctcccc gtccttttcc tgggctcaat cgtttccaaa
      541 accgcggatc gccccgcaat cggggcaggt ccagttgatg ccgttgcagg tcatgcccca
      601 gaagttttct tccatgcatt cggagcaaat catgaagccg cagggacagg tccaacagaa
      661 gggttggtcc gtatcgcatg tggcgcatcg gtactggatc ggccttcgcc atttggcccg
      721 ctttggttcc atggttccgt ctccgttctt caccgggacc gggccaggct ggtgacgaag
      781 agcttcatct cggaactctt gccgggggtc tcggacgtcg tgtcgccgac ccttccttcc
      841 cggatttgga tgaaccgggc atcggtcttg aacaccatgg aggagaaaac ccccttgacc
      901 gtggtcccgg aacacaccag cccctgcacc tggacggaag gggggcggcc cgcggcggag
      961 ttccattcgg tcagggcggt gagttcatcc cggaggtcgc ggattgcctg atccattccg
     1021 cgaagctgtt ccttgtgccg ggccagcttg cctcggatct cgtcctgttc ttcgaagaga
     1081 gcgtccagtt cccgggcggc ggatccgatg acggcctcga tctccttgag atactcttcc
     1141 gcctgggcca gttgggccgt gtccttccgt tcccgaagct cccggatttc gctctgaacc
     1201 tgcctcaact ccaaccccgt ccggtcctga acctgggcca gttccccgat tctcgcctcg
     1261 atcacggcgt cccgttctcg cagaggccga accagggaag cgtgcccggc cctctccgcc
     1321 tcgtgccggg caaggctctc cttgagccgg tcgatttccc gttcggcgag gggatcgatc
     1381 ccgatgacga gaacgctcgg ggcggatcgc tcgagcccca cctgggcggc ttcgattcct
     1441 cttcgagccg tgatcctgga ggaaacgacg gctccccgca ggcaggtgag cttgccggag
     1501 atgctgattt tcgaatccac gacccctttg tccaccacca cgtttcccat ggcttcgatc
     1561 tgggaatcgt agatgtggac ggcgtttacg ttgccctggg accgcacctt ggcccccagg
     1621 atgccgccgt acacaaccac gtccccggag acgtcgatct gggccttgcc gatctcacgg
     1681 gcgatgaggg cggatccctt cactcgaaag ccgtcttgga cgactccgtg gacggtgatt
     1741 tttccctcgt attcgatgtt gcccgtctcg aagctcacgt cgccggggat caccagttcg
     1801 ggcagaacgg acagcctacc gaggagggaa agctggggaa ggccgtcgac ccgggccgtc
     1861 acccgcctgc ggtcggggga gatctcggtg cccgatccgc aaagcagggt gagttccttg
     1921 ctcttgggga ccgcgatttc gtttccaaag acgtccgttc cgggagtttc ggggacccgg
     1981 ggggtctttt ccgcgaggag gtcgcccttc ttgacccggg ggatttctcc caggtccttg
     2041 aaatcgacct tgccctcccc ttcgaggggg gcgagggttt tgggacgagt gggaaagcag
     2101 taccggattt cggcgtcttt tcccggctga ggatcggttc cccgggcgat tctccacacc
     2161 tgcttgcggg acgggggctg ttggagaagg gcttcgagct cgaggggatc gagaagcccg
     2221 tagacgatcc cctgggcttc cagggcgtgg gtcaggagct cgacgccgtg cccttcggaa
     2281 agagaagcct tgatccgcag gtaggctcga agtttgtcct cggagacgag caggtcaaag
     2341 gattcttcct ccaggagaat gtgggtgtcg tcgggcaaag tctcgctctc gatccggatt
     2401 tgcgcccggc ctttcggttg ggcggaagcg ggtcccgggg gtccggaacc ggttgcatcc
     2461 ggaggtgtcg gggcgggcgc ggaaggtgcc gccggggcgg gcttcgaggg aggggccggg
     2521 gaggacgccg aggggagctc cggagatttc atgcgggcca ggagggcgtc cctgaccccg
     2581 gccttgagcc acccttgttc cacgagcagg tcccccaggg atcgaaatac ccgggtttcc
     2641 ttatagattc tcgcctgcat ttgagaggcg gcgtccgcct gttcccgggt gaccagcccc
     2701 cggcttaccg ccaggcggcc gaagagccga tccggcttgc gcagggagag aaagtcggct
     2761 gccagctgga gagcgtccag ctgtcttcga gagatccacc cacggtccag gaggacctgg
     2821 cgcaggggcg ggggggtccc cgattcctcc cgttcccgct ggaaggacaa agcgtctcgg
     2881 acctgttgtt ccgtgaggaa cccgtgctga acggcgagct ttgccaggac ggcgtcgtcc
     2941 tcccgggcgg aacccatgtt tgcctgctgg gccgttgagc caattgtatc ggtcacggca
     3001 ttcccccttt gttcccgtag cgcaggtgaa gaggaccttt ggggcccgta cgcagaaaac
     3061 aggcgcttct tcgttccaat cctccgggtt tccggtatgg ggcctggccg aaaattagcc
     3121 ctcgattccg ggcctctccc accgggggaa aaaacgtgtt ttcacctcca tggatgggaa
     3181 agagcccggg gaggaaggac ggatcggcca tgcacaagca cccgctgatc atatcccatc
     3241 atctctgttt tcgtccaccc tgtcggaatc ctttacggag atttgaaagt gatcttgtga
     3301 aatcatgagg aaaccgggca aggaatgacc ttgtgccgcc cgatggaaag ctcgtgcagg
     3361 gggatgagaa tgtcggccat gtcccggatt cggttggcgc tctcgtaggc ggcaacggca
     3421 tcgaggtgca ctcccggagc cactgccggt cccttggccg ggaagttcat ctcgttgcag
     3481 cagaaccccg tgatgatcgc caggccgttg cgggtctgaa cggcaatgga ctgcccaccg
     3541 ggggtgtgcc cgggggtcag cagcagatcg atgccgtccc tgaagggggt gtccccctcc
     3601 accagctcga ccttcaatcc gtccaggaga tcgggatagt accggtggtc gatgggatga
     3661 ggatccagga agaattcgta ttccgctttt tgcaccacga tccgggcttg aggacacttg
     3721 tagtcgtttt cgcaatggtc gttatgcaag tgggtgtgca ggatcacgtc gatgtccccg
     3781 gggctgaggc cgtgcttttc cagggcgctc tcgaactcga ggatctccag tccgtaccgg
     3841 gtggagacct cttcgggcac cacgaactgc tcgagtcccg tatccaccag gatccgctct
     3901 tctcccccca ggatcaggaa ggcgtagatg ggaatccaga ttctcttccc gtaccccctc
     3961 tggtacgtca tgatgccctg gtcggtttca ttggctccga ccgtcagcgg atggatcgtg
     4021 tattccttca tgctgtctcg ttctccttta cggcttttgt tcatccctta gttgcagggg
     4081 cggggttttc ggcagggttc cggggacgtc ttcgggaacc ggggacttca gggtgattcc
     4141 agggtcctgc gaaggtcccg ggtgaggcgg tccagctctg ctctcaggtc ctcgaacgcc
     4201 cggggaaagt cctcgacctt cccgtcccgg gcaagcacct cgagccggaa agcggcatcc
     4261 atggttcccc gggcccccaa attgcccgag gtccccttga gagtatgggc cgctcgttgg
     4321 acccgggcgt catcgcccag cccgatggcg tcccggattt cctcgaggag ggaagccgat
     4381 tcctcgagga atacgagaac caggccctgg aacagttcga tgtctccgtc caggttccgc
     4441 aaagtgccgg ccgggtcaaa agattccgac ttgaacgtca tattttcatc ccttttctgg
     4501 tacattatac gcgatatggc cccagagagt gagccgaaat caaaggaaac gacatgaccc
     4561 ccttggtctc acggccggaa aaggccgaca acccgaccca gcttctcacg gccatcatgg
     4621 aatccgtcac ggagggaatc atcaccatcg acgaacgcgg catcatcgag atggtgaacc
     4681 cggcagcgga gcggatgttt gggtattctc cgggagagct cgtgcggaaa aacatcgagg
     4741 tcctggtgcc cgagcctcac cggggccgcc atgacgaatt cctctcccgg ttcctcgaaa
     4801 ccggggcgac gaaaaccccc ggtccggttc gagagaccga ggcctgtcga aaggacggtt
     4861 ccgtctttcc cgtctcggtg gccgtgagtc ggttcgagat cggcggtcgg ctcaaggtcg
     4921 tcggcgtgtt ccgcgatatt tcggaacgaa agaggatgct tgcaaacctc gtggaaagcg
     4981 aggcccgcta tcgcgacatg gcgatcctgc aggcggccgt cctggatgga gccaactact
     5041 ccatcatcgc gactttcccg gacgggatca ttcggagctt caatgccgtg gcccagcgct
     5101 ggctgggata ttccgccgag gaggtcgtgg gaaaagtcac ccccgagatc tttcatgatc
     5161 ccgtcgaatt ggcccggtat gccgaggagc tctcgaagga gttggggcgg ccgatccatc
     5221 cgggcttcga ggtctttgcg gccaaagccc ggcagggatc ccccgtggaa agggaatgga
     5281 cctatgttcg gaaagacggc agccgctttc ccgtgctcct gtccctgacc cccctgctgg
     5341 atgccgacgg ggtcatcacg ggttttctgg gcattgccgg ggacatcacc gaccgaaaac
     5401 gggccgatga agccctggca aggcttgcct ccatcgtcga atcctcggac gacgccatca
     5461 tcggcaagtc cctggacggg gtcgttctca gctggaacaa gggcgccgag aggctttacg
     5521 gctaccgcgc ctcggaaatg ctgggccgac ccatctccgt cctgaccccc ccgggccgtt
     5581 cggatgagct cgatgggatc ctggaccgga ttcgacgggg agaacgggtg gaacatttcg
     5641 agacccttcg gatcggaaaa gacggcagag ccatcgatgt ctccctgacc gtttccccca
     5701 tcacggaccc ggcagggcga atctgcggcg tgtccgccat cgcccgggac atcaccgggc
     5761 gcaagaaggc cgaggaacag ctgagaaggt tgtccatggc cgtggagcaa agccccgtca
     5821 gcgtggtcat cacggatatc acgggaagaa tcgagtacgt gaacccgaag ttcaccgaag
     5881 tcaccgggta ttcctccgaa gaggccatcg gtcagaaccc gcgcatcctg aagtccggag
     5941 acaaaccccc cagcgaatac aagaagctgt gggaaaccat caccgcgggc ggcgaatggc
     6001 ggggggaatt tcacaacaag aagaagaacg gggagctcta ctgggaatcg gcatccattt
     6061 ctcccatcaa ggacgacaag ggcaatatca cccacttcat cgccgtcaag gaagacatca
     6121 cgtccctcaa ggaggcccgg gaggaactgg ccaagctttc cctggttgcc agcaagaccg
     6181 acaacgccgt catcatcacc gatgccgaag gtgtcatcga gtgggtcaac gacggcttcg
     6241 tgcgcctcac cgggtacact ctcccggaag tcgtgggccg caaaccgggt tccgtcctcc
     6301 agggtcccct caccgaccgg ctcacggtac agagaatccg ggagtttctc aaggcccggg
     6361 tgcccttcac cgaggaaatc ctcaattacc acaaggaggg gttcccctac tgggtctcca
     6421 tgaacgtgac ccccattttc gacgattcgg ggcgactcat ccggttcatt tccatcgaaa
     6481 gcgacgtcac cgagcgcagg cacgccgagg aggccctgcg aaaggccaaa gaggcggcgg
     6541 atgccgccag ccgggccaag acggagttct tgtccagcat gagccacgag atccggactc
     6601 ccatgaatgc catcgtcggc atggcggagc tcctgtggga aagtcccctg aacgacgaac
     6661 agcgcaaata tgtccagatg tttcggtctg cgggggatac cctcctcaat ctcatcaacg
     6721 acattttgga cctgtccaag gtggagtcgg gccagatcga gctcgaagcc atccccttcg
     6781 atcttgcgga agtgatcgac aggacgtgcg aggtcatggc cgtgcgggcc cacgagaagg
     6841 ggctcgaact ggcgtgccgg atcatgcccg ggctgcccac tcacctggtg ggcgatcccg
     6901 gacgccttcg acaggttctc accaacctca tcggaaatgc gttgaaattc accgaacaag
     6961 gcgagatcgt ggtcgaggtc aagtgccggg acctcggagg ggcgggctgc acgggagacg
     7021 gaggccgcca aagggacctt ctcttttcgg ttcgggatac gggcatcggg atcgagccgg
     7081 agaaacagga ggtcattttc gaaaagtttg cccaggcgga cacgtccatc actcggaagt
     7141 acggcggcac cggcttgggc ctggccatca cccggaaact ggtggagctc atggggggga
     7201 agatctgggt cgaaagcacg gtgggagagg gcacgacctt ctccttcagc atcccgttcg
     7261 aggaggcgcc cgctcccgtc cccaaggaac ccttggcgga agtggatttg gccggggtca
     7321 aagtcctggt gatcgacgac aactccacca accggttgat cctgagagag accctgtccc
     7381 ggtggggagc cccggtgttt gccgtcggca gcggcgagga aggcatcgca gagctccaaa
     7441 aggccaagga agccgggaac ccctacctat tggtgctgct ggatcaacgg atgccggaaa
     7501 tggacgggtt ccgcgtggcg gagcggatca agaatctgcc cgacctggcg ggcacagccg
     7561 tcatcatgct gacttccgac ggcatgagcc ttgccacggg ccgttcccgg gaattgggaa
     7621 tagtgagctg cctgaccaag ccggtcaagc agtcggagct ccgggaggcg atcaacctga
     7681 ccctgggccg gttcgttccc cctgcccccc gggagacccc tcccgccccc gagggcctgg
     7741 ggaccttgaa catcctgctg gtcgaggatt cctcggccaa ccgcttcctc atccaggcct
     7801 ttctcaaaaa caccggatgc cgcatcgaca ccgcggaaaa cgggcagatc gccgtggaga
     7861 aattcctttc gaaccgctac gatttggtga tcatggatat gcagatgccc gtcatggacg
     7921 gttacacggc aactcgaacc atccggagca tcgagaagga agaagggttg gcccccgtgc
     7981 ccatcgtggc cctaacggct cacgccttga aggaggatgt ccaaaaaagc ctggatgcag
     8041 gctgcgacgc ccacctgaac aagcccatca agaaggcgga actgatcgga gccatcatgg
     8101 gctatctgaa gggcccgcct ccacggccat gacgcccgga tgctctcgga atcgttgaga
     8161 ttcggattga atcaactctg cgcggaagat tgggaccttc cccggcggca agacatggaa
     8221 gatatccgtg atccccgttg ggggaacgtt ctcggcaacc cattgaaatt tcagaccatt
     8281 cgagttcggc taattttaga taacaatttc agcaatttcc gtttgattac cgaaggattt
     8341 gagggcattc tgaagcatcg aaacggcgga aagagaaccc ggcgggcacc gacccgctcg
     8401 tcgggggccg tcgagtgccg aggaggcaac gatcgtcaac cacgaacctg agttgagcga
     8461 ttccgagagc atcagggcgt catgcccgcg acggcgggca tccggcgggc atgcatgctc
     8521 tttccggatt cccggatgcg cgggaaggaa gaaacgaggt tcatgccggt caaatcgctc
     8581 aaccaaggac gatgtcggca ggggcgagaa tgcagctgga aggctacaaa atggggggag
     8641 tgctggtggt caggccgctc ggccgacgga tcgatgcttc cgagtcgccg gacttcaagg
     8701 gcaagatggt ggactggatc aaccagggcg agacccgcat tgttttggac ctctccgggg
     8761 tggatttcat cgacagcagc gggttgggag cggtggtctc ggttctcaag accctggggg
     8821 gcgaaggcga gctgactatc ggcggcttga gggagccggt ggggagcctt ttccggctga
     8881 cccggctgga cagggtcttc cggatctatc cttcggcgga agatgccgtc aaggccatgt
     8941 cccaagtgtc ctaggacgcc gtttcgggaa accacgcact tgtattcgtc ctcccggcca
     9001 atgagggggt ccggaaggga ttgaagaacc ggctttcggc cgccggccga aacggcgacc
     9061 cgaaggttcg ggcctgtccc cgagcgcagg aaacggggtc ccgggaactg cccgctgagc
     9121 gggccggagt ccgagggctc ggcggacgcc tacggtgtag tcgacatggg tagaggactt
     9181 cgcaacagcc agaacctcag cttcgtgggg atgctcgccc tgacggccct ggtcaccgtc
     9241 tttgcctacc aggagatcca ccgggaatgg gtccttttca gaaaggccga aaccgctttt
     9301 tccaagaagg ggttttccga ggccgtttcc ctgtacgaag aagccattgc ggccggggcc
     9361 cgcacgccca cggcgtaccg tcgcctggga gaatcctgcc tggccttggg caggttccgg
     9421 gaagccctcg agacctacga acagctgctc cgtctttacc ccgaagacct ttccgccatg
     9481 aagaaccttg cggccctcta cgatcagttc gatcgtcccg aggacgccat tgccctttat
     9541 ctccggtacg aaagcaggtc ttccctggac gtgccgtccc tgcttcgcct ggcgaatctc
     9601 taccagcgaa agcaggactg ggagtcggcc gggtcccttt acaggaagat cctggatcga
     9661 gacccagggt ccgttgcggg acgcatgggc cttgcggaac tgctcagctg gacgaggcgc
     9721 tacgaggaag ccgaggccct ctaccgcgac gtcttgaaag aagaccccgg ccatcgtcgg
     9781 gcccggattc aactggcacg ggtgctgagc tggagcggac gttttgagga gtccatcgag
     9841 gagtatcgaa cggccctcaa tccgtcttcc ggaaaatgaa tctcaagggg agtatcccga
     9901 tgaaaaaaca tgcccgggtt gagcggttcc gaaggcattc gcccgtcatg cccgagaagg
     9961 agggcatccg gagggtatgc acggctcttg agggttcctg cttgcgcggg agtgacaaaa
    10021 cgggacttgg atccatgcaa tcgctcgatc cggggcacgg acggtaccga tccctgccgt
    10081 tggtttttct cgggctcctg ctctgctttc cccccgtcgg gtacggggcc tcccgggagg
    10141 caaccgtggg gggccccggg caggtcgggt ccccggaaac cggggttgct cccatccagc
    10201 cggtccagcc cgcccccgaa agtctgcagg aagagattcc cgactggaag gcccggtggg
    10261 aactggcacg cctgctcagc tacgtcaaac gctacgagga atccctggtc gaatacggaa
    10321 gggtcctcga ggagaaaccc gacctgtggg aagcgaaggt cgagacggcc caggtgctgt
    10381 actggagcgg gcggcaatcg gaagcctccc ggacgctgga agaaatcccc gccgggcacc
    10441 tggatgaaag ggctcggctg ctcatggcgg acctgtacgt ggctcggaag gcctacgaca
    10501 aggccgaacc catcttccgt gcccatttga aatcccatcc cgaagactac cgggcggcgg
    10561 tgaagctggc ggatgttttg agctggaccc ggcgctacga agaagccatt ctgctttaca
    10621 aggaagtcct gaacgcaaga cccgacgaca tccagattcg gcgcaaatat gcctatgtgc
    10681 tcatctgggc cggccgacca tcggaagcgg ccgcggaatt gagaaagacc ctcaaaccgt
    10741 agagatcggt ttccacgttt tccggggagt tgcccgtgtc ttccagaata tcgtccctgc
    10801 gacggggcct cgtctttgtg atcttcgccc tggtttcgtt ctcggccttt tggtttccgg
    10861 ccggggcggg agcggtgggc gagtcccggt ccgacgagga cttccccggg gcaatctccg
    10921 atttcgaggc acgtttgacc cttgcccggc tcctttcgta ccgaggcgat acgctggacg
    10981 agg
//
LOCUS       DAOOVC010000130        10861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3916_length_10861_cov_6.291597, whole genome shotgun sequence.
ACCESSION   DAOOVC010000130 DAOOVC010000000
VERSION     DAOOVC010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10861
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3916_length_10861_cov_6.291597"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLM79_14035"
     CDS             <1..572
                     /locus_tag="PLM79_14035"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOV87480.1"
                     /translation="ILVGDVSGKGIASALTMAKLTSDFRLLAIRQKEPARLVEEINHL
                     FCLRSRRGMFVTLVYLVLDPGNGEVDFINAGHVPPVLWNAARDRFELLKKPGGPPVGI
                     VPGQVYSPGKLHLEPRDCLLLSTDGLIEAKNPQGDRLGWERLESAVRRGDSRVETVYA
                     RTMESLGQFVEDCPQADDITLVLVGVEES"
     gene            572..1015
                     /locus_tag="PLM79_14040"
     CDS             572..1015
                     /locus_tag="PLM79_14040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87481.1"
                     /translation="MSLEEIHGPVFLEVPSDPASLFLVRCLTDRLTQRLGFPQSEVDR
                     VVLAVDEACANIIRHAYGGRLGERIHLTYLVGEERLEIRIRDFGSGTDPVHFKPRELA
                     DVRPGGLGIHFIRSAMDRVEYETPPDGGTLLTMIKLRQPKEGGKE"
     gene            1012..1359
                     /locus_tag="PLM79_14045"
     CDS             1012..1359
                     /locus_tag="PLM79_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOV87482.1"
                     /translation="MTIEVKERTPGVSVVALAGDVDMSTSPRVREVLLPCFQQGTSHV
                     VVDLSRVSYIDSSGIATLVEGLQLSRRGGIRFTLVGVSPSVDAVFELAYLKDVFEMAP
                     GMDELFQGELPRG"
     gene            1352..2152
                     /locus_tag="PLM79_14050"
     CDS             1352..2152
                     /locus_tag="PLM79_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV87483.1"
                     /translation="MAEGPERDRRFGPVILFLQHLTSIAYLFRETLRWMLVKPFQGKP
                     LRVRAIIAQMDEVGVKSIPIVFLVSFVIGIILVLQTAYQLEKFGAVTYAASLASVALT
                     REMAPLLTAIVLAGRVSAAFTAELGTMLVTEEILALEVMAIAPVAYLVVPRFIAMLVM
                     LPCLTALADVVGMFGGYVVGTTAVGIRSTLYIQTSLDALLLKDILTGLFKSFVFAGII
                     AMVGCYMAFVVRGGAEGVGRSTMISVVTSLISIIIADCFFTTVFFLFL"
     gene            2149..2964
                     /locus_tag="PLM79_14055"
     CDS             2149..2964
                     /locus_tag="PLM79_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87484.1"
                     /translation="MNANGQTESIIRVRDLVKSYGGRVVLDGICLEVEKGKVTAIMGG
                     SGCGKSTLLRHLIGAVRPDSGEIWVKGQDITKFGESEMEAYRKRFGMLFQGGALLNSL
                     SVKDNIALPLREHTRLDEKIIGVMVKMKLELVGLRDFEHLKPAQLSGGMQKRVALARA
                     LALDPEIVFYDEPTSGLDPVVSGVIDQLIKNLSRRMNITSVVVTHDTRSAFKIADKMV
                     VLYRGKVVAEGPPEAIQTSPDPLVQQFIAGSPEGPIPLRQSSRDYLEDLLDME"
     gene            2998..4020
                     /locus_tag="PLM79_14060"
     CDS             2998..4020
                     /locus_tag="PLM79_14060"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOV87485.1"
                     /translation="MEVFRTETRVGLLILAALVLVSVGIFITSDIRELWESKRTIELL
                     FPYADGITRGSPVWYAGFEVGEVSGIRIAPGTTDRIAVTAKIDPRARVRKDSRIDIRS
                     LGMMGAKYVEITPGSPDSPELEPGETLEGKGPSSLSEILETGQQVATRLAALVEETRT
                     LVHEVRTESSLKEAIQNANGLLVDLRERGKDLQPIMRKVGSFADSLDQTGANLKKTSG
                     EGGKELTALLKELRDTNRDLQKRIENVELQLTKTLAQIDRGFSEAEGFARGAKTLLVS
                     NEKDITSFIKHLKETTRHLEILTEDLRAHPWKVVWKENGTYDHAVPSTAAENREKGRI
                     GPQGKK"
     gene            4071..4994
                     /locus_tag="PLM79_14065"
     CDS             4071..4994
                     /locus_tag="PLM79_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87486.1"
                     /translation="MIGWDGIWIPGRSRQRRGWSAPACALGLLAVLGLGGCAAGKTDC
                     YSAGVDHQAKGRLPEAARDYRRAMEVNPGDPRPRFNLAVLYQDEGKLDEAARLYRTIV
                     DQDPGYAPAWANLASIREKEGDMSGAEHLYRKAMEVSPGDVTAAGQYGFFLVRRERLG
                     EAEIVFQEIIEKDRSFSNAHYGLGVIHRARGENSQALDCFENAMVYSPSDLMAHLETA
                     ELLVAAGNRAKAVRVLTRATKIAPERASTWLLLGRLLREEGRWKEGERALEQALNMGA
                     PKAEVYTELSQVYERLAEDASRAAADTGAGN"
     gene            5075..5950
                     /gene="glyQ"
                     /locus_tag="PLM79_14070"
     CDS             5075..5950
                     /gene="glyQ"
                     /locus_tag="PLM79_14070"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOV87487.1"
                     /translation="MTFQELILALDKFWSDRGCLIQQPYDLEVGAGTFNPATFLRVLG
                     PEPYRVAYVEPSRRPTDGRYGENPNRLQHYYQYQVILKPSPPDSQGLYLESMKSFGID
                     PLEHDIRFVEDDWESPTLGASGLGWEVWLDGMEITQFTYFQQVGGIALSPVSVELTYG
                     LERIAMYLQGVNNVYDLIWSGKVTYGDVHHQGEVEWSHYNFEEADVGMLFQLFNMFEA
                     ESLRLNEKGLVLPTYDYCLKCSHVFNLLDARGAISVTERTNYIARVRNLARLVAHAYV
                     AQREQMGYPLLKKWG"
     gene            5960..8044
                     /gene="glyS"
                     /locus_tag="PLM79_14075"
     CDS             5960..8044
                     /gene="glyS"
                     /locus_tag="PLM79_14075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOV87488.1"
                     /translation="MEATVGKEFYLEIGSEEIPAGYILPALEAISAQMVRFMDENRIG
                     HGSPSTTGTPRRLVLRVPDVALRQEACSTEIVGPPKQVAFDARGNPTKAAEGFARGQG
                     VSLEAVRIKETPKGEYLCIVKEEEGLPTRELLERMLPDFIAHIPFPKSMRWGSLSISF
                     ARPIHWIVALLGEEVLDFSYGEIPSGNQSLGHRFMSPQWLTVRDYATHLEQLKKHFVY
                     ADTAERKELVRRGIEAAAQRVGGRILEDEELLDEVTQLVEYPHPIVGEFETKYLDLPP
                     ELLITVIKKHQRYFAVTDQDGKLMQYFVTVANTVPKDDAMVAAGNARVVRARLEDARF
                     YYVEDQKVRLEDRAEQLKGVVFHSKMGTSWEKMERFSALARWIGQKLAPETLPIISRA
                     ALLCKADLLSGVVGEFPELQGVMGRAYARLQGEPEEVARAIFEHYLPVRAGGPIPETT
                     AGAVLSIADKMDTIVGCFGVGLIPTGTADPFALRRQTLGIIRIILEKPYRLSLTQLIN
                     EAVPPLESKMTEPQETVKEGVVEFFRGRLHHFLVSQEGFSPDVVEASLAAGIEDLVDA
                     VRRTRALAVFKARPDFESLATAFKRVVNIIKEPETTPVQPELLQNAAEQALFAAFKDA
                     EGVVEGCLAQSDYSAALEAMARLKEFIDGFFDSVLVMDKVEGVRRNRLGLLTRIRDLF
                     ARVADFRKIQTG"
     gene            complement(8299..8997)
                     /locus_tag="PLM79_14080"
     CDS             complement(8299..8997)
                     /locus_tag="PLM79_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87489.1"
                     /translation="MKDFSLTQVADLLPPGEIWVLGLGKFGMLAWKRLEKRFPRASFV
                     LVEEREEKTRGIQERGSLKIVARDAIGFLLDARVPGEVWIIPAVPVHAAFRWLLGRLE
                     PPRAASSIPLPREIDPRVPNPLRSGEETIYTSFAAFVCPDGCDEPDEICTYTKKARPG
                     NLYEELSRLEIPGFRTHVLRSLQLAPGVGGYPGRSLEGLHEEVLKTPGNHLVATSCRC
                     HGVINALNWRETGA"
     gene            9165..9557
                     /locus_tag="PLM79_14085"
     CDS             9165..9557
                     /locus_tag="PLM79_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HOV87490.1"
                     /translation="MGNIFSILDKIAEERILEAMERGEFDDLPGKGKPLRLEDDSQLP
                     PDLRMAYKILKNADCLPPEVQLRKDIRTAQSLLAGIQDAQEKYRQIRKLNYLIMKLNM
                     SRRASVLLEEDQYYCEKVLDRLDSKKSD"
     gene            complement(9618..>10861)
                     /locus_tag="PLM79_14090"
     CDS             complement(9618..>10861)
                     /locus_tag="PLM79_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOV87491.1"
                     /translation="GFNIRQPGIYYGELDHYRYVVAPNDEGEFDYPEGNSNVMNHYAG
                     SGGVPLNSLYKKFLFAYYFGDRNLFFTTKTNQRSKVLFRRNIRERILSLTPFLRLDHN
                     PYIAVTSRGLFWIQDAYTVSDGYPNAAMHVDGDFRFNYIRNSVKIVVDAYHGTVDYYV
                     FDPRDPVIRAYARMYPGFFKDKGAMPPDLLSQVRYPQDLFNAQMSIYAKYHQKDPEVF
                     YQQEDAWEFAATTLGKEVIPIKSYYLTLDLIERGQPGFILLSPMSPKGRANLRALMVV
                     SAQGAHYGKMVAYNFPKGELIYGPSQVNALIQQDTKVAEQFTLWDQIGSRVERGKMII
                     LPIGNVVIYIQPIYLIASTQLNIPELKRLIMTQGQVVVMEPSLEEAYKKLEERLKEES
                     LRIERRYAPAGVKPAPAEPPQ"
BASE COUNT         2187 a         3235 c         3385 g         2054 t
ORIGIN      
        1 ggatcctggt gggggacgtt tcgggcaagg gaatcgcctc tgccctcacc atggccaagc
       61 tgacgtcgga ctttcgcctg ctggccattc gccagaaaga accggcacga ctggtggaag
      121 agatcaacca ccttttttgc ctgcgcagtc gaagggggat gttcgtcacc ctggtctacc
      181 tggttttgga ccccgggaac ggagaggtgg atttcatcaa cgccgggcac gtgccccccg
      241 ttctctggaa tgccgcccgg gatcggtttg agctcttgaa aaagccgggg gggccccccg
      301 tggggattgt gccgggccag gtctattccc ccgggaaact gcatctcgag ccgagggact
      361 gcctgctcct ctcgacggac ggcctgatcg aggccaagaa cccccaaggg gatcgcctgg
      421 gttgggagcg gctcgagtcc gctgtgcgcc ggggcgattc ccgggtcgag accgtttatg
      481 cccgcaccat ggagtccctg ggacagtttg tcgaggattg cccccaggcg gacgacatca
      541 ccctggtgct cgtgggagtc gaggagtcct gatgtccctg gaggagatcc acggcccggt
      601 gttcctcgag gttccgtcgg acccggcctc ccttttcctg gtgcgctgct tgaccgaccg
      661 gctgacccaa cgccttgggt tccctcaatc ggaggtcgat cgggtggtcc tggcggtgga
      721 cgaggcctgt gccaacatca tccggcatgc ctacggcggg cggctggggg agcggattca
      781 tctgacctat ctcgtggggg aggagcggtt ggagatccgg attcgggatt ttggttcggg
      841 gacggacccg gtgcatttca agcccagaga acttgcggat gtccgccccg gaggtctggg
      901 gatacacttc atccggagtg ccatggatcg agtcgagtac gagactcctc ccgacggagg
      961 aaccctgctc acgatgatca agcttcggca acccaaggag ggcgggaagg aatgacaatc
     1021 gaggtcaagg aacgaacccc cggggtgtcc gtggttgccc tggcggggga tgtggacatg
     1081 agcacctcgc cccgggtaag agaggtcctc ctgccgtgct ttcaacaagg gacttcccat
     1141 gtggtggtgg acctgtcgcg ggtgtcctac atcgacagct ccggcatcgc cacgctggtc
     1201 gaggggcttc agctctctcg aagaggcggc atccggttca ccctggtggg ggtgagtccg
     1261 tcggtggatg ccgtgttcga gctggcctat ctcaaggacg tcttcgagat ggctcccggt
     1321 atggacgagc tttttcaagg ggagctcccc cgtggctgaa ggtcccgaac gggatcgccg
     1381 gtttggcccg gtgatcctct tcctccagca cctcaccagc attgcctacc tgtttcggga
     1441 aaccctgaga tggatgttgg tcaagccttt tcagggaaag cccctccggg tgagggccat
     1501 cattgcccag atggatgagg tgggagtgaa gtccataccc atcgtgttcc tggtttcctt
     1561 tgttatcggg atcattctgg ttctccagac cgcttatcag ctcgagaaat ttggggccgt
     1621 cacctatgcg gccagcctgg caagcgtggc cctgacccgg gaaatggctc ccctccttac
     1681 ggccatcgtc ctggcgggcc gggtgagtgc ggcctttacg gccgaactgg gaaccatgct
     1741 ggtgaccgaa gaaatcctgg ccctcgaggt catggccatt gcgccggtgg cctacctggt
     1801 ggttccccgg ttcattgcca tgcttgtcat gctgccctgt ctcaccgccc tggccgacgt
     1861 cgtcggaatg ttcgggggct acgtggtggg caccaccgcc gtgggaatcc gctcgacgct
     1921 ctacatccag accagcctgg atgccctgct tctcaaagac atcctcaccg ggctgttcaa
     1981 gagctttgta tttgccggga tcattgccat ggtgggatgt tatatggcat ttgtggtccg
     2041 gggaggtgcc gaaggggtgg gcaggtccac catgatttcc gtggtgactt ccctcatcag
     2101 catcatcatt gccgactgct ttttcacaac ggtgtttttc ctgttcctat gaacgcaaac
     2161 ggtcaaacgg aaagcatcat ccgggtgagg gacctcgtca agagctacgg ggggcgggtg
     2221 gtcctggacg ggatctgcct ggaggtggaa aagggcaagg tgacggccat catgggcgga
     2281 agcgggtgcg gcaagagcac cctgctgcgc cacctcatcg gggccgtccg tccggattcc
     2341 ggggaaatct gggtgaaggg acaggacatc accaaattcg gcgagagcga aatggaagcc
     2401 taccggaagc ggttcggcat gctgttccag ggaggtgccc ttctcaactc gctttcggtc
     2461 aaggacaaca tcgccctgcc cctccgggag cacacccggt tggacgaaaa gatcatcggg
     2521 gtcatggtca aaatgaagct cgaactggtg ggcctgaggg acttcgaaca cctgaagccc
     2581 gctcaactca gcggcggcat gcagaagcgg gtggccctgg cccgggccct ggccctggac
     2641 cccgagatcg tgttctatga cgagcccacc tccggcctgg atcccgtggt cagcggcgtc
     2701 atcgaccagc tcatcaagaa cctgagccga aggatgaaca tcacctcggt ggtggtgacc
     2761 cacgacacgc gaagcgcttt caagattgcg gacaaaatgg tagtgctcta ccggggcaag
     2821 gtggttgccg aaggcccccc cgaggcgatc cagacctccc cggacccgct ggtgcaacag
     2881 ttcatcgccg ggagtcccga aggacccatc cccttgcggc aatcgagccg ggattacctg
     2941 gaagacttgc tggacatgga atgaggtgaa cctacccagg ctggagaaaa aaaccgtatg
     3001 gaggtctttc gaacggaaac gagggtgggc cttttgatcc ttgccgcgct ggtgctggtg
     3061 agtgtcggga tcttcattac cagcgatatc cgtgagttgt gggagagcaa gaggaccatc
     3121 gaactcctgt tcccttacgc ggacgggatc acccggggct cccccgtgtg gtacgctggg
     3181 ttcgaggtgg gggaggtttc cggcattcgc atcgctcccg ggaccacgga ccgcatcgcc
     3241 gtgaccgcca agatcgatcc ccgggcgcgc gttcgcaagg actcgcgcat cgatatccgg
     3301 agcctgggaa tgatgggagc caagtacgtg gagatcactc ccggctcgcc ggattcgccc
     3361 gagctcgagc ccggagaaac cctcgaagga aaagggccgt cttccctgtc ggaaatcctc
     3421 gagacgggac aacaggtggc cacgcgcctc gccgccctgg tcgaagaaac ccggaccctg
     3481 gttcacgagg tgcgtaccga gtcgtccctc aaagaggcca tccaaaacgc caacgggctg
     3541 ctggtggatc tccgggagcg gggaaaggac ctgcagccca tcatgcggaa ggtgggaagc
     3601 tttgccgatt ccctggacca gaccggcgcc aacttgaaaa agacctcggg agaagggggc
     3661 aaagagctca ccgcactgct caaagagctc cgggacacca accgggatct tcagaagcgg
     3721 atcgaaaacg tggaactgca gctgaccaaa accctggccc agatcgaccg cggcttttcc
     3781 gaggccgaag ggttcgcccg gggggccaag accctgctcg tttccaacga aaaggacatc
     3841 acctccttca tcaagcacct caaggaaacc acgcgccacc tcgagatctt gacggaagac
     3901 ctgagagctc atccgtggaa agtggtctgg aaggaaaacg gcacctatga ccatgccgtg
     3961 ccttcgacgg cggcggaaaa ccgggagaaa gggaggatcg ggccccaggg caagaagtag
     4021 cctgttgcgg cccgcgggtt tcctccccac gcaaatcagg agatcagctc atgatcggtt
     4081 gggatggaat ttggatcccc gggcgttccc ggcagcggag agggtggagt gcccccgcat
     4141 gcgccctggg tcttctcgcc gtcctcgggc tgggcggatg tgccgcgggg aagaccgatt
     4201 gttacagtgc cggagtagac caccaggcca aggggcgcct tcccgaggcg gcccgggatt
     4261 accggcgggc catggaagta aatcctgggg acccccggcc ccggttcaac ctggccgtcc
     4321 tataccagga cgaggggaaa ttggatgaag ccgcccgcct ctaccggacc atcgtggacc
     4381 aagaccccgg gtacgccccg gcctgggcga acctggcctc catccgcgag aaagagggcg
     4441 acatgtcggg tgcggaacac ctctaccgga aagcaatgga agtgagcccc ggagacgtta
     4501 cggccgccgg gcaatacggg ttcttcctgg tccggcggga gcgactgggg gaggcggaaa
     4561 tcgtttttca agaaatcatc gagaaggacc gctccttttc caatgcccat tacggcctgg
     4621 gggtcattca ccgggcccgg ggggagaact cccaggccct ggattgcttc gagaacgcca
     4681 tggtttacag tccctcggac ctcatggccc atctcgagac ggcggaactc ctggttgcgg
     4741 ccgggaaccg ggccaaagcc gtccgggtcc tgacgcgggc gacaaaaatc gccccggaac
     4801 gggcaagcac ctggttgctc ctggggcgtc ttctcaggga agaaggcaga tggaaggaag
     4861 gggaaagggc gttggaacag gccctgaaca tgggggcccc caaggcggag gtgtacacgg
     4921 agctcagcca ggtttatgag aggcttgccg aggacgcctc ccgggccgcc gcggacaccg
     4981 gtgccggcaa ctgatccgac ccgccggtcg aaccggactc gtacccggcg gacggtgtct
     5041 ttttcatgaa ttctcagtcg ttaggagaga gtatatgact tttcaggaat tgatccttgc
     5101 attggacaag ttctggtccg atcgagggtg tctgatccag cagccctacg atttggaggt
     5161 gggggcgggt acgttcaatc cggccacttt ccttcgggtg ctcgggcccg agccctaccg
     5221 cgtcgcctac gtggaaccgt cccgccggcc caccgacgga cgctacgggg aaaatcccaa
     5281 ccggctccag cactactacc agtaccaggt gattctgaag ccttctcccc cggactccca
     5341 gggcctgtac ctcgagagca tgaaaagctt cggaatcgat cccctggaac acgacatccg
     5401 gtttgtggag gacgactggg aatcccccac tctgggagcc tcgggcctgg gctgggaagt
     5461 ctggctggac ggcatggaga tcacccagtt cacctacttc cagcaggtgg gaggaatcgc
     5521 tctgagccca gtgagcgtcg agctgaccta cggcctcgag cggattgcca tgtacctgca
     5581 gggggtcaac aacgtgtacg acctcatctg gagcgggaag gtcacctacg gagacgtcca
     5641 ccaccagggg gaagtggagt ggtctcacta caacttcgaa gaggccgacg tggggatgtt
     5701 gttccagctg ttcaacatgt tcgaggccga atccttgcgc ctcaacgaaa agggactggt
     5761 ccttcccacc tacgactact gtctcaagtg ttcccacgtc ttcaacctcc tggacgcccg
     5821 gggggcgatc agtgtgacgg aacggaccaa ttacatcgcc cgggtgcgca acctggcccg
     5881 tctggtggcc catgcctacg tggcccaacg ggaacagatg ggatatcctc tcctcaagaa
     5941 atggggctag gcggaaagga tggaggctac agtgggaaaa gaattctacc tggaaatagg
     6001 aagtgaagaa atcccggcag ggtacatcct tccggcgctg gaagcgattt ccgcccagat
     6061 ggttcgcttc atggatgaaa accgcatcgg tcacggaagt ccctccacta cgggaacccc
     6121 gcgcaggctg gtgctccggg ttcccgacgt ggctctgcgc caggaagcct gttccaccga
     6181 gatcgtgggg ccccccaaac aggtggcctt cgacgctcgg ggaaatccca ccaaggcggc
     6241 cgaaggcttc gcccggggac agggagtctc cctcgaggcc gtacgcatca aggagacgcc
     6301 caaaggggaa tacctgtgca tcgtgaaaga ggaagaaggg cttcccaccc gggagctcct
     6361 ggagcgcatg ctcccggatt tcattgccca catccccttt cccaagtcca tgcgctgggg
     6421 gagcctcagc atctcctttg cccggcccat tcactggatc gtggcgctcc tgggagagga
     6481 agtcctggat ttttcctacg gggaaatccc aagcggcaac cagtccctgg ggcatcgctt
     6541 catgagcccc caatggctca ccgtaaggga ttacgccacc cacctcgagc aactcaagaa
     6601 acacttcgtg tacgcggaca cggccgagcg aaaggagctc gtccgccggg gcatcgaggc
     6661 cgcggcgcaa cgggtgggag gccgaatcct ggaagacgaa gagctcctgg acgaggtcac
     6721 ccaattggtg gaataccccc atcccatcgt gggggaattc gaaaccaagt acctcgatct
     6781 gcccccggaa ctgctcatca ccgtcatcaa gaagcaccag cgatacttcg ccgtgaccga
     6841 tcaagacggc aaattgatgc aatacttcgt gaccgtggcc aataccgttc ccaaggacga
     6901 cgccatggtg gccgcgggaa atgcccgggt cgttcgagcc cgcctcgagg acgctcgttt
     6961 ctactatgtc gaggaccaga aggttcgtct cgaggaccgg gccgaacagc tcaagggagt
     7021 ggtgttccat tccaagatgg gaaccagctg ggagaaaatg gagcgatttt ccgccctggc
     7081 ccggtggatc ggccaaaagc tggcgcccga aacccttccc atcatttccc gggcggccct
     7141 tctctgcaag gcggacctcc tgtccggggt ggtgggggag ttccccgagc tccagggagt
     7201 gatgggacga gcgtatgcgc ggttgcaggg agaaccggaa gaggtggccc gggccatctt
     7261 cgaacactac ctgccggtgc gggccggcgg tcccatcccg gaaaccaccg caggagccgt
     7321 cctgagcatt gccgacaaga tggacaccat cgtggggtgc ttcggcgtgg gactcattcc
     7381 cacgggcacc gcggaccctt tcgcccttcg caggcagacc ctgggaatta tccggatcat
     7441 cctggaaaaa ccctatcgcc tttccctgac ccaattgatc aatgaagccg ttccgcccct
     7501 cgaatccaag atgacggaac ctcaagaaac ggtgaaagag ggggtggtcg agttcttccg
     7561 aggcaggctg caccatttcc tggtaagcca ggaagggttt tcccccgacg tggtggaggc
     7621 atccctggcg gcgggcatcg aagacctggt ggatgccgtc cggcgcaccc gggccctggc
     7681 ggtcttcaag gcccgtcccg atttcgagag cctggccacg gctttcaagc gggtggtgaa
     7741 catcatcaag gaacccgaga cgacgccggt gcaaccggaa ctgctccaaa acgccgcgga
     7801 acaggccctc tttgcggcgt tcaaggacgc cgaaggagtc gtggaaggct gcctggctca
     7861 atcggactac tctgcagccc tggaagccat ggcccgcctg aaggaattca tcgacgggtt
     7921 ctttgattcg gtcctggtca tggacaaggt cgagggcgtt cgccgcaaca ggctggggct
     7981 cctcacccgc atccgggacc tcttcgcccg cgtggcggat tttagaaaga ttcaaacggg
     8041 ctgaaccagg ccccgagggg aaggctggga agctggggcc cgggagggag aaaaccccct
     8101 gccggtgccc tcgatttccc accgtcccga cctgccgcct cccggcactc caatttctta
     8161 tcccctatct acctcgagcg atcagccttt ggcattttgc cattagcgtt gaactttcca
     8221 gcagttgagc tgttttggct cccacccaac aggtgaccac tctgccaaag agcgaacggc
     8281 caacggcgaa tccctcgatt aggccccggt ctccctccag ttcagggcgt tgatcactcc
     8341 gtgacagcga caactggtgg cgacgagatg gtttcccggg gttttcagga cctcttcatg
     8401 aaggccctcc agggaccttc ccggatatcc ccccactccc ggagccagtt gtaggctgcg
     8461 caggacatgg gtgcgaaagc ccgggatttc caggcggctc agttcctcgt aaaggttccc
     8521 gggccgcgct ttcttggtgt aggtgcagat ctcgtcgggc tcatcgcacc catcgggaca
     8581 aacaaaggcg gcaaagctcg tgtagatcgt ctcctccccg cttctcaggg gattgggcac
     8641 ccgagggtcg atttctcggg gcaaagggat cgaggaagcg gcccttggag gttccagccg
     8701 acccaacagc caccgaaagg ccgcgtgcac cgggacggcg gggatgatcc agacttcccc
     8761 gggcacccgg gcatccagga gaaaaccgat cgcgtcccgg gccacgatct tgagggaccc
     8821 cctttcctgg atcccccggg tcttttcttc tctttcttcc acgaggacaa aagaagcccg
     8881 gggaaacctc ttttccagtc tcttccaggc cagcataccg aacttcccca atcccaggac
     8941 ccagatctcc ccgggaggca gcaagtccgc cacttgtgtg agggaaaaat ctttcatttt
     9001 ctcttttcct cggatttgat acattcccgg aataaagttc cgagcctcct ctttcggcct
     9061 gccgccgaca tccggactcg agggaaaggc cgagaagaaa ggatttcccg ccgggtgtcc
     9121 gaggcttgat tccgggagca cgaaaaaggt caaggaccgc ggacatgggt aacatattta
     9181 gtattctcga taagatcgcc gaagaaagaa tcctggaagc catggaacgg ggggagttcg
     9241 atgatctgcc gggaaagggg aaaccgctcc ggctggaaga cgacagccag ttgcccccgg
     9301 atctgcgcat ggcctacaag atcctgaaga acgccgattg cctccctccc gaggtccagc
     9361 tgagaaaaga catccggact gcccagtcgc tcctggccgg aatccaggac gctcaggaga
     9421 agtatcggca gatccggaaa ctcaactacc tgatcatgaa attgaacatg agccgccggg
     9481 cctccgtcct cctggaagag gaccagtact actgtgaaaa ggtcctggac cgcctcgatt
     9541 ccaagaaatc cgactaaccg acccgccgcc tcccccacct cccggcgatc ccgttattgg
     9601 aacaattgga aaccccgtca ctgcgggggt tccgccgggg cgggcttgac gcccgcgggg
     9661 gcgtaccgcc gttcgatccg caaagactct tcctttagcc gttcttcgag cttcttgtag
     9721 gcttcctcga gggaaggttc catgaccacc acctgcccct gggtcataat gagcctcttg
     9781 agctcgggaa tgttcagctg ggtggaggcg atgagataga tgggctggat gtagatcacc
     9841 acgtttccaa tgggaaggat gatcatcttg ccccgttcca cccgcgatcc gatctggtcc
     9901 cacagggtga actgttcggc cactttggta tcctgctgga tgagggcatt cacctgggaa
     9961 gggccgtaga tcagttctcc cttggggaaa ttgtaggcca ccatcttgcc gtaatgggct
    10021 ccctgggcac tgaccaccat cagggcacga aggttggccc ggcccttggg actcatggga
    10081 gacagcagga taaaacccgg ctgtccccgc tcgatgaggt ccagggtcag gtagtaggac
    10141 ttgatgggaa tcacctcttt gcccagggtc gtggccgcga attcccaggc atcctcctgt
    10201 tgatagaaca cctcgggatc cttctggtgg tacttggcat agatgctcat ctgagcgttg
    10261 aacagatcct ggggatagcg cacctgggac agaaggtccg gaggcatggc tcccttatcc
    10321 ttgaaaaacc ccggatacat cctggcatag gctcgaatga cggggtcccg gggatcgaag
    10381 acgtagtagt ccaccgtccc gtggtaagca tccactacga tcttcaccga gttgcggatg
    10441 tagttgaacc ggaagtcccc gtccacatgc atggcggcgt tgggatagcc gtcggacacg
    10501 gtgtaggcgt cctgaatcca gaagagtcct cgggaagtca ccgcgatgta gggattgtgg
    10561 tccaggcgca ggaagggggt cagggagagg atccgttccc ggatgttgcg ccgaaaaagg
    10621 accttgctgc gttggttggt cttggtggtg aagaagaggt tccgatcccc gaagtagtag
    10681 gcaaacagga acttcttgta caggctgttc aaggggaccc ctccgctccc cgcgtagtgg
    10741 ttcatgacgt tggagttgcc ttcgggatag tcgaactcgc cctcatcgtt gggagcgacg
    10801 acataacggt agtgatccag ttccccgtaa tagatgcccg gctgccgaat gttgaagccg
    10861 a
//
LOCUS       DAOOVC010000131        10798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3936_length_10798_cov_7.153775, whole genome shotgun sequence.
ACCESSION   DAOOVC010000131 DAOOVC010000000
VERSION     DAOOVC010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10798
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3936_length_10798_cov_7.153775"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1588)
                     /locus_tag="PLM79_14095"
     CDS             complement(236..1588)
                     /locus_tag="PLM79_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV87492.1"
                     /translation="MRLTANRTLRRFFLGGVLLASVVAAAMYYPSRAGNSRESFQVGT
                     VQRTDLLSTISATGTVEPEELVDVGAQVAGKIVAFGEDREGKTVDYGSPVEEGTVLAK
                     IDDVLYQADVAQAEAQLGQAKAGLERARADLAQLQAKLAQAERDWARARKLGPSEALA
                     QSAYDAALSAHEVAKANVGVGRAAVTQAEKAVAQAEAAWMRAKQNLGYCTIKSPVKGI
                     IIDRRVNIGQTVVASLNAPSLFLIAKDLTRLQVWVAVNEADIGSIRPGQDVSFTVDAF
                     PGEDFEGKVNKIRLNASMTQNVVTYTVEVNTDNPDGRLLPYLTANVKFILGERQGVLT
                     VPNAALRWTPKTEDVDPEIRRTLASDSPDRRERSRQAGGERAEIGTVWVRNNGFVRPL
                     FVGIGLSDGSRTQIEGEEIREGLEVVVGREVKQGAAPPVSASPFTPKLPGRGRPGGRP
                     "
     gene            complement(1969..2388)
                     /locus_tag="PLM79_14100"
     CDS             complement(1969..2388)
                     /locus_tag="PLM79_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87493.1"
                     /translation="MKRWKLWVGLVSLFLSGMVAGALGTVVISEHMALEVLVGRGPIV
                     EKVLKKLSRELSLTESQRERIAEIVCRTHKELTALRERNKPERDEIMGRSREAMKAEL
                     SAPQQKKLDEFFEQEKTRWAHREHGKAGGGGRKGLCD"
     gene            complement(2480..2812)
                     /locus_tag="PLM79_14105"
     CDS             complement(2480..2812)
                     /locus_tag="PLM79_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87494.1"
                     /translation="MKPPRDDVRRIETALIHYHRNAAGGPAGNPSWQRKLMSGVRRES
                     LSGYDLLQNGFPVGRTVYRFALVTCLTVLLMALIIMDSNVNTQYELAELILSDAAAVD
                     MARSFGVL"
     gene            complement(2809..3414)
                     /locus_tag="PLM79_14110"
     CDS             complement(2809..3414)
                     /locus_tag="PLM79_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOV87495.1"
                     /translation="MGSSEGEAQDWDVITRVVEGDVNAFELLVDRHKARVFGIVYRHV
                     PGDRVEDVAQEVFLEAFRSLKSFSGASPLDHWLSRIAVRCCADFWQRRRGSRETPVSS
                     LSPDSEAWLEKVLTAPSREAFHREAARKEAAEVLAYALDRLSPEDRMALTLVHLEGCS
                     AKQAAELLGWSTISVRVRTHRSKKKLRKILSDLLGNRRGET"
     gene            4057..5361
                     /locus_tag="PLM79_14115"
     CDS             4057..5361
                     /locus_tag="PLM79_14115"
                     /inference="COORDINATES: protein motif:HMM:NF023165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HOV87496.1"
                     /translation="MRRKPRCCSMLIGFLVLCLSLPPGIWAQGSGSGDTFSQQELDQL
                     LAPVALYPDSLLAQILVAATYPLEVVEAARWVKRNKGQSPEALNNALDAKTWDLSVKA
                     LVPFPDVLAMMSEKLDWTQKLGDAFLAQESEVMDTVQKLRRKAKEKGNLKTTAQQKVV
                     VEGESVIIEPANPQVIYVPTYNPTVVYGTWWWPDYPPYYYYPAGAVVTAGMIGFAAGI
                     AVGSAWNWGWGHWDWNHHTVNVNVNRNVNINRNDIRRTDVRTDKWKHEVNHRQNVPYR
                     NQANRDRYGQKGKDSPDNRRDYRGHNPDRADRGSREINQQRGDRASRDMDRARGDRSD
                     FGSRSRPTSDSALRGLQQREHSNAFGNYGRGADVRMDSDRGRQSREASSFGGGRQSFD
                     RGGFDRGGRSGFDHGGFGGGRSGGGGFGGGRGGGGGGHGGRR"
     gene            5451..6425
                     /locus_tag="PLM79_14120"
     CDS             5451..6425
                     /locus_tag="PLM79_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HOV87497.1"
                     /translation="MLSSNRHPARGSKIRLSAVVFLAVAFLLVSFGGNSWAAPAKKQP
                     QQTFPTAEAAVQALIQAMKDHDQKQLLAVLGLQGKHLITSGDPVADREGRERFLKLYE
                     EKNQLTKEGDSKVVLEIGSELWPMPIPIVKVGDAWLFDTQAGKEEILNRRIGRNELSA
                     MKVCLAYVDAQLEYSAKDRDGDGILEYAQKIVSTEGKQDGLFWEATSGAEPSPLGPFA
                     AKAAGEGYTKKKPGGKPSPYHGYFYKVLTAQGKHARDGAREYLVKNNMIGGFALVAYP
                     ARYGSSGIMTFIVNHDGIVYEKDLGKNTAKIARSMRLFDPDSTWKKVQ"
     gene            6705..9194
                     /locus_tag="PLM79_14125"
     CDS             6705..9194
                     /locus_tag="PLM79_14125"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOV87498.1"
                     /translation="MLDITSPVCSPEAPRTLDQIQSLGTLVEDIHRHIVSTLGNEVYG
                     RNKFRYFHGLAYSIRDRLIRLWINTQREYYDSMAKRVYYLSMEFLPGRFLMNYLTNLC
                     MEEGCREELEKLGLALDDLEEVEWDAGLGNGGLGRLASCYLDSIASLKIPGYGYGILY
                     DYGIFYQRIVNGYQEESCDNWRRQGSPWEIQRRENLYEVNFYGRSEAYRDGEGNLRYR
                     WVDTENIMAMPCDILIPGYRGNHVTNMRLWAAQSSRDFNLSVFNLGDYAGAMHDKIMS
                     ENISKVLYPSDKEEQGRELRLKQQYFLVSATFQDIMRRHKKQHSSMAGFADHVAVQLN
                     DTHPSIAIAELMRILLDVERLPWEQAWEICTGTFAYTNHTVLPEALETWPVELIGRLL
                     PRHLEIIYEINHRFLKEVAARYPGDADRLRRMSLIQEGSERRVRMAHLAVVGSHSVNG
                     VAALHTQILKDSLFRDFHQFYPGKFKNVTNGVTPRRWILQANSALSRLITEAIGPGWV
                     NHLERLRDLVPMAEDPEFRRAFARAKMDNKKRLARYVLRKMSMGIDPHTLFDCQFKRL
                     HEYKRQLLNILHVITRYNRIKDDPDGEMTPRTVLMGGKAAPGYFQAKLIIKLFNSVAE
                     VVNHDPDVGGRLRLLFLPNYCISQSEKVIPAADLSQQISTAGMEASGTGNMKFALNGA
                     LTIGTLDGANVEIREEVGPENFFLFGLTAGEVDQLRQTGYDPYGYYAGDPELKRVVDQ
                     IASGVFSPDEPSLFMPIVENLLRNGDYFMVLADYRSYVDTQARVDELFRNKEEWTRRT
                     VLNTANMGRFSSDRAVMEYARDIWGVQPLGS"
     gene            complement(9235..10485)
                     /locus_tag="PLM79_14130"
     CDS             complement(9235..10485)
                     /locus_tag="PLM79_14130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV87499.1"
                     /translation="MTEETTAPEEQSVFFTRQPIFDARRSIWGYELLGCEIKDGIHPV
                     FPGQGGGTVWSAGTYVGLQEAMERGKKIMVGFDKAGILAGLPYALPPSSGVVRVLPGP
                     TPASELDGALRKLRSDGYRIAVEILPRSPLPVHLMAQADILAWDLGRSTPESALFHGI
                     KEHKGNLLARGVKTGEQFQAARHMDFTLFQGSFFKESERVPNRRLGSNEVTRLNLFRL
                     MEGVDPDFKALAAAVRSDVSLSFRLLSYLNSASFGFRQNIQSIDQAVLLLGWQKLKSW
                     LRAVLLVDMAGKDEVPRELAALSLLRGKFLEVLATRVDYWGFNPGTLFLMGLFSLLDA
                     ILGMPMEKVVELLPLDARLKAALQREPNNEYRPLFELLEHLEDADWPALEVLAKNLCV
                     DLQLLKNVFAQAREWSLGFFAQGA"
BASE COUNT         2194 a         3427 c         3174 g         2003 t
ORIGIN      
        1 cccgacccgg atgtgcccat gagagccacg aattctcccc gggagacgct gagggaagcc
       61 cccttgagca cggggacgct gttttccccc aagaggtaca ccttggtgac gtttttcaat
      121 tccacaaatt ccacggattc ttctccccgc ccgcatccaa cccaaattcc gggccgttgc
      181 gccggaatgg gggactcgat gagatccttc gggacaagcg tgtcggcgcg ttgggctacg
      241 gtcgaccgcc gggcctgcct cgtccgggca acttgggggt gaacggactg gccgaaaccg
      301 ggggggcggc cccttgcttg acttcccggc ccacaacgac ctcgagtccc tcccggattt
      361 cctctccctc aatctgcgtc cggctgccgt cgctgaggcc gatcccaacg aacaggggcc
      421 gaacaaaccc gttgttgcgg acccacacgg tcccgatctc cgccctttct cctcccgcct
      481 gccgcgatct ttcccttcgg tccggcgaat cggaagcaag ggttcgacga atttcggggt
      541 ccacgtcttc cgtcttcggg gtccatcgca gggccgcgtt gggtaccgtc aaaaccccct
      601 gtcgctcccc caggatgaac ttcacgttgg cggtgaggta gggcaacagc ctgccgtcgg
      661 ggttgtccgt gttcacctcg acggtgtagg tcaccacgtt ctgggtcatg gacgcattga
      721 gacgaatctt gttcaccttg ccctcgaagt cttctccggg gaaggcgtcc acggtgaagc
      781 tcacgtcctg gccgggacgg atgctgccga tgtccgcttc gttgaccgca acccaaacct
      841 gaaggcgggt gaggtccttg gcgatgagaa acaggctggg agcgttgagg ctggcgacca
      901 ccgtctgacc gatattgacc cgccgatcaa tgatgatccc cttgaccggc gacttgatgg
      961 tgcagtaccc gaggttctgc ttggcccgca tccaggccgc ttccgcctgg gctacggctt
     1021 tctcggcctg ggtcacggcc gccctgccca ccccgacatt ggccttggcc acctcgtggg
     1081 cggaaagggc cgcatcgtag gcgctctggg ccagggcttc cgaaggaccc agcttgcggg
     1141 ctcgagccca gtctctttcc gcctgggcaa gctttgcctg cagttgggcc aggtccgccc
     1201 gggcccgttc caggcccgct ttggcctggc ccagctgagc ttccgcctgg gctacgtccg
     1261 cctggtagag gacatcgtca atctttgcca ggacggtgcc ctcttccacc ggagacccgt
     1321 agtcgacggt cttgccctcc cggtcctctc cgaaggcgac gatttttccg gccacctggg
     1381 cgcccacgtc caccaactcc tcgggctcca ccgtcccggt ggcgctgatg gtcgacagca
     1441 gatccgtcct ttgaaccgtc cccacttgga aggattctcg agaattcccg gcccgggagg
     1501 gatagtacat ggcagccgcc acaaccgaag caagaaggac ccccccaaga aagaatcgtc
     1561 tcaacgtgcg atttgctgtg agtctcatag atccgccgtc tactccccag tgaattcggc
     1621 aagtcccgga tcgaaaccga acccggtgcc ggggttttca caaatccctt gcggagcaag
     1681 gattgctgcc ttttccgaga aacacggccc catctcggcc gtcatccgct tccccattca
     1741 ccgattctcc catgagccgg ggcccgggaa gcttcgaagc ccggcgtgcg acccgaggac
     1801 gcaacgggac gcaagaagag cgatttttcc cgttccatgg gtgtgcataa agctcgcccc
     1861 taggcctgcc gacgaaccga ccccgcccgc cgggagcgcc gttggatggg gaaggaccat
     1921 caagaatcgc tcggttaggg ggaatcccca gggccttggg acaccgtttc agtcgcaaag
     1981 gcccttcctg cctccaccgc cggctttccc gtgctctctg tgcgcccagc gagtcttttc
     2041 ctgctcgaag aactcatcca gcttcttctg ctgcggtgcc gagagctcag ccttcatggc
     2101 ctcgcgactc cgacccatga tttcgtctcg ctcgggtttg ttcctctccc gcagagcggt
     2161 caattccttg tgggttcggc agacgatctc ggcgattcgt tctcgctggc tttccgtcaa
     2221 gctcagctcc cgggaaagct tcttcaggac cttctcgacg atgggtcctc ttcccacgag
     2281 gacttcgagg gccatgtgtt cggaaatcac cacggttccc aaggcgccgg caaccatgcc
     2341 ggacagaaac aaactcacaa gtcccaccca caatttccat cttttcaccg gccaacgcct
     2401 cctttcaccg gacgcccaaa gaaggacccg tcgacgaaac cacgtcggaa gaaatcaaag
     2461 tcccgcatcc ggcctcaaat caaaggaccc cgaaagaacg ggccatgtcc acggcggccg
     2521 cgtcactgag aatgagttct gcgagctcgt attgggtatt gacgttcgaa tccatgataa
     2581 tgagagccat gagcaacacc gtcagacaag tcaccagggc aaagcgatag accgttcggc
     2641 ccaccgggaa tccgttctgc aagaggtcgt aacccgacag ggattcccgg cgaaccccgc
     2701 tcatgagctt ccgctgccag gacggattgc ccgccggtcc cccggccgca ttgcgatggt
     2761 agtgaatgag agcggtctcg attctacgaa cgtcgtctct gggcggcttc atgtctcacc
     2821 tcgcctgttt cccaacaaat ccgagagaat ctttcgaagc ttcttcttcg acctgtgggt
     2881 gcgaacccgc accgagatcg tgctccaccc cagtagttcc gcggcctgtt tcgccgaaca
     2941 accctcaaga tgcaccaggg tgagtgccat ccggtcctcg ggcgaaagtc gatccagggc
     3001 atatgccagg acctccgccg cttccttccg tgccgcttcc cgatggaaag cctctcgaga
     3061 cggcgccgtc agaaccttct ccagccatgc ttcggaatca ggcgataggc tgctgaccgg
     3121 cgtctctcga ctcccccgcc gccgttgcca gaagtccgcg cagcatctca cggcaatgcg
     3181 ggaaagccag tggtccaagg ggcttgcccc cgaaaaggac ttgagggacc ggaacgcctc
     3241 gaggaaaacc tcctgggcca cgtcctcgac gcggtccccg gggacgtgcc ggtagacgat
     3301 gccgaacacc cgggctttgt gacggtccac cagcagctcg aacgcgttca cgtccccctc
     3361 gacaacccgg gtgatcacgt cccaatcctg agcttcgccc tcagaagatc ccataggtcc
     3421 gcattccgtt cccaccgtat cctgtcccga ccctgtcact ccttaagtct cggccgtcct
     3481 gcaaattgtt acaagcctga accgcctgaa tgcggttttt tcggacggac cgagaaacac
     3541 gggcacttcc cttgggaaac caggccctgt gcccgtaaga tttgtggtct tcggcttctt
     3601 gaaccctcca tccccgacgg tcggtccgcc gggaagaagc ttcaggtccg ctctggggaa
     3661 ttgaaatccc ggcaccggtc attcgcggct ttacatgctc tccccgacca tttaaactct
     3721 ttgccgctcc tattcaccat caggaacctt tgaccgattt ggccggtatg aatcccgttt
     3781 cggctttgct gcgaaagggg gactttcatt caaaggggcg cagcccgttg tctgagtgac
     3841 tcccgcgcaa gggggacccg gatagatcgt gcatgcccgc cggatgcctg ccttcacggg
     3901 catgacgccc gaatgctctc ggaaccgctc gaaccgtgag gaatacgggc cgctcaggaa
     3961 tcttcacaaa atgaattgta tcccgtcctt gtccgaaaag cgccttttgc aaaaaaagga
     4021 tcacgttccc ggtgcatcca ggaaggagaa aggatcatga gaaggaaacc gcgctgttgt
     4081 tccatgctca tcggctttct ggtcttgtgc ttgagcctcc cgcccggcat atgggcccag
     4141 ggctccggca gcggcgacac cttcagccag caggagttgg atcagctcct cgcccccgtt
     4201 gccctttacc cggattctct cctcgcccaa attctcgtgg cggccaccta tcctctcgag
     4261 gtggtggaag cggcccgctg ggtgaagaga aacaaggggc aaagcccgga ggcgctcaac
     4321 aacgccctgg atgccaagac gtgggacctg agcgtcaagg ccctggttcc ctttcccgac
     4381 gtcctggcca tgatgagtga aaaactcgac tggacccaga aattggggga cgctttcctg
     4441 gcccaggaga gcgaggtcat ggatacggtc cagaagctgc gccgcaaggc caaggagaag
     4501 gggaacctga agaccacggc tcaacagaag gtcgtggtgg aaggggagtc cgtgatcatc
     4561 gagccggcca atccccaggt catctacgtt cccacctaca atcccacggt ggtttacggg
     4621 acctggtggt ggcccgacta tccgccgtat tactactatc cggcgggagc ggtggtcacg
     4681 gcggggatga tcggctttgc cgcgggcatc gccgtcggat ccgcctggaa ctggggctgg
     4741 ggccactggg actggaatca tcacacggtc aacgtcaacg tcaaccgcaa cgtgaatatc
     4801 aatcgcaacg acattcggcg caccgatgtc cgtacggata agtggaagca tgaagtgaat
     4861 caccggcaga acgtgcccta tcggaaccag gccaatcgcg atcggtacgg ccagaaaggg
     4921 aaagactccc cggacaatcg gcgcgattat cgcggacaca atcccgacag ggccgaccga
     4981 ggttcccggg aaatcaacca gcagcggggg gaccgggctt cccgtgacat ggatcgcgcc
     5041 cggggggacc gctccgattt cggaagcagg tccaggccga cctcggactc ggcactccga
     5101 ggcctccaac agcgcgagca ttcaaacgct ttcggcaatt acgggcgcgg tgcggatgtc
     5161 cgtatggact cggaccgggg tcgtcaaagc cgggaggcct cttccttcgg cggcggacgc
     5221 cagagctttg atcgaggcgg tttcgatcgc ggcggccgca gcgggtttga ccacgggggt
     5281 tttgggggcg gtcgcagcgg cggcggaggt tttgggggcg gtcgtggcgg cggaggcgga
     5341 ggccatgggg ggcgcaggta acgagggccc cgatgctcga atccctattt gcggatggtg
     5401 cgaacaacca ggaaccctga gaacaacccg gtcgaaggga ggaccacaac atgctttcca
     5461 gcaatagaca tccggcaaga ggatcgaaaa ttcgcttgag cgccgtggtt tttttggctg
     5521 tcgcctttct ccttgtttcc ttcggcggca attcatgggc cgctcccgca aagaaacagc
     5581 cccagcaaac cttccccacg gcggaagcag ccgtccaggc cctaattcag gccatgaagg
     5641 accacgacca aaagcagctc ctggctgtcc tggggctcca gggaaaacac ctcatcacct
     5701 cgggggaccc cgtggccgac agggaagggc gcgagcggtt cctgaagctc tacgaagaaa
     5761 agaaccagtt gacgaaggaa ggagacagca aggtggtgct cgagatcggt tccgagcttt
     5821 ggccgatgcc catccctatc gtcaaggtgg gcgacgcctg gctgttcgat acccaggccg
     5881 ggaaggagga aatcctcaac cgcaggatcg gtcgcaacga actcagcgcc atgaaggtct
     5941 gcctggctta cgtggacgct caactggaat actcggccaa agaccgggac ggggacggga
     6001 tcctggaata cgctcagaaa atcgtcagca ccgagggcaa gcaggacggt ctgttctggg
     6061 aggcaacatc gggtgccgaa cccagtcccc tggggccctt tgccgccaaa gccgcgggtg
     6121 aaggctacac caagaaaaag cccggcggca agccttcccc gtatcacggc tatttttaca
     6181 aggtcctgac cgcccaggga aagcacgcac gggacggtgc acgggaatac ctggtcaaga
     6241 acaacatgat cggcggcttt gccctcgtcg cctacccggc ccgctacggg tcctcgggca
     6301 tcatgacctt catcgtcaac catgacggaa ttgtctacga gaaggacctg ggcaagaaca
     6361 cggcaaagat tgcccggtcc atgagactat tcgatcccga cagcacctgg aagaaggttc
     6421 aataggacct ccggcggcct tcaggtcccg gtggggaccc caactcgacc gatcccgtgc
     6481 acccggcggg aaacggcacg acgagtcgcg ggcggggaga aaccccgccc cgtctctcct
     6541 ttccgcaccc atgccggggg ggtcccggct gcccgcaccc caaggtccct ccccgccctg
     6601 aagatggaat tgacaacccg ggacaaatcc cccattctct agactcgaca acccgattcc
     6661 tttcaggaag accctcgctc cgaaccacag aagagtacat tcccatgctc gacatcactt
     6721 cgcccgtgtg ttccccggaa gcgccccgga ctctcgatca gatccagagc ctgggcaccc
     6781 tggtcgagga catccaccgc cacatcgttt ccaccctggg aaacgaggtc tacgggcgga
     6841 acaaattccg ctattttcac ggcctggctt acagcatccg ggatcggctc atccgattgt
     6901 ggatcaacac ccagcgggaa tattacgaca gcatggccaa gcgggtgtac tacctctcca
     6961 tggaattcct ccccggcagg tttctcatga actatctcac caacctgtgc atggaagagg
     7021 gctgccggga agagctggag aaactgggcc tggccctgga cgacctggaa gaggtggagt
     7081 gggacgcggg gctgggcaac ggcgggttgg ggaggctcgc gtcatgctac ctcgattcca
     7141 ttgccagcct gaaaatcccc ggttacgggt acgggatcct ctatgactac ggcatcttct
     7201 accaaagaat cgtgaacgga tatcaggagg aaagctgcga caactggcgc cgccagggca
     7261 gtccctggga aatccagcga cgggagaacc tctacgaggt caacttctac ggaaggagcg
     7321 aggcctaccg tgacggggaa ggcaatctgc gctaccggtg ggtggacacg gaaaacatca
     7381 tggccatgcc ctgcgatatc ctgatccccg gctaccgcgg caaccacgtg accaacatgc
     7441 gcctgtgggc cgcccagtcc agccgcgact tcaacctaag tgttttcaac ctgggcgact
     7501 atgccggggc catgcacgac aagatcatga gcgaaaacat ttccaaggtg ctctacccca
     7561 gcgacaagga ggaacagggc agggagctgc gcctaaaaca gcagtatttc ctggtgtccg
     7621 ccacctttca ggacatcatg cgccgccaca aaaagcagca cagcagcatg gccggttttg
     7681 ccgaccacgt ggccgtgcag ttgaacgaca cccatccttc catcgccatc gccgaactca
     7741 tgcggattct cctggatgtg gaaaggctgc cgtgggagca ggcctgggag atctgcaccg
     7801 gcaccttcgc atacaccaac cacacggtgc tgcccgaggc cctcgagacc tggcccgtcg
     7861 agctcatcgg tcgcctgttg ccccggcacc tcgagatcat ttacgaaatc aaccatcggt
     7921 ttctgaaaga ggtggcggcc cgatatcccg gggacgcgga tcgcctgaga cggatgtccc
     7981 tcatccagga ggggagcgag cgccgggtgc gcatggccca cctggccgtc gtggggagcc
     8041 attccgtcaa cggggttgcc gccctgcaca cacaaatcct caaagacagc ctctttcgag
     8101 actttcacca gttctacccc gggaaattca agaacgtcac caacggcgtt acgccccgtc
     8161 gatggattct gcaggccaat tccgcccttt cccggctcat caccgaagcc atcgggcccg
     8221 gctgggtgaa ccacctcgag aggctcaggg atcttgttcc catggccgag gacccggaat
     8281 tccggcgggc ctttgcccgg gccaagatgg acaacaagaa gaggctggcc cggtacgtgc
     8341 ttcgcaagat gagcatgggc atcgatcccc acaccctctt cgactgccag tttaaacggc
     8401 tgcacgaata caagcgccag ctcctgaaca tcctccacgt cattacccgg tacaaccgga
     8461 tcaaggacga ccccgacggg gaaatgactc cacgcaccgt cctgatggga ggaaaggccg
     8521 cccccggcta tttccaggcc aagctcatca tcaagctttt caattccgtg gccgaggtgg
     8581 tcaaccacga ccccgatgtg ggtggtcgcc ttcggctgct ctttctgccc aactactgca
     8641 tctcccagtc cgaaaaggtc atcccggccg cagacctctc tcaacagatc tccacggccg
     8701 ggatggaagc ctcgggaacg ggaaacatga aatttgccct caacggggcc ctgaccatcg
     8761 gcaccctgga cggcgccaac gtggaaatcc gggaggaagt gggtcccgaa aacttcttcc
     8821 tgttcgggct cacggccggg gaggtggacc agctgcggca aacgggctac gacccttacg
     8881 ggtactacgc gggggacccg gaactcaaac gagtcgtgga tcaaatcgct tcgggcgtct
     8941 tctcccccga tgagccgtcc ctcttcatgc ccatcgtgga gaaccttctg cgaaacggcg
     9001 actacttcat ggtgctggcg gactatcgca gttacgtgga tacccaggcc cgggtggatg
     9061 agcttttccg caacaaggag gaatggacgc gaaggaccgt cctcaacacg gccaacatgg
     9121 gtcggttttc gagcgaccgg gcggtgatgg agtatgcccg ggacatctgg ggtgtgcagc
     9181 cgttggggtc gtagggccgg aatcgttctc ccacctcgat gccctcgaag ggggctatgc
     9241 gccctgggcg aaaaagccca gggaccattc ccgggcctgg gcaaaaacgt tctttaggag
     9301 ctgcaggtcc acgcagaggt ttttggccag gacctcgagg gcgggccagt ccgcgtcctc
     9361 gagatgctcc aaaagctcga agagcggccg gtactcgttg ttgggctccc gttggagggc
     9421 ggccttgagc cgggcgtcca ggggcagcag ctcgacgacc ttttccatgg gcatgcccag
     9481 aatggcgtcc aggagggaaa acaggcccat gagaaagagg gtgccgggat tgaaccccca
     9541 gtaatccacc cgggttgcca ggacctcgag aaactttccc cgcagcaaag agagtgccgc
     9601 caactcccgg ggaacctcgt ccttgcctgc catgtccacc aggagcacgg ccctcagcca
     9661 gcttttcagc ttctgccaac ccagcagtag gacggcctgg tcgatggact ggatattctg
     9721 tcggaaacca aaggaagcgc tgttcagata ggagagaaga cgaaagctca gggacacgtc
     9781 cgaacgaacc gccgccgcca gggctttgaa gtccgggtct acgccttcca tgagacgaaa
     9841 aaggttcaga cgggttacct cgttggaacc gagcctgcga ttgggcacgc gctccgattc
     9901 cttgaaaaag gacccctgga acagggtgaa gtccatgtgc cgggcggcct gaaactgctc
     9961 cccggtcttg acccctcgcg ccagcaggtt tcccttgtgc tccttaatcc catggaaaag
    10021 ggcggattcg ggtgtgcttc gacccaggtc ccaggccagg atgtccgcct gggccataag
    10081 atggacggga agggggcttc gaggaaggat ctcgacggcg atgcggtagc cgtcgcttcg
    10141 cagcttccgg agagcgccat ccagctccga cgcgggcgtc gggccgggca acacgcgcac
    10201 cactccgctc gagggcggaa gagcgtaggg caatcctgcc aggatgccgg ctttgtcgaa
    10261 gcccaccatg atcttcttgc cccgctccat ggcttcctgc aagcccacgt aggtgccggc
    10321 ggaccagacg gtcccgccgc cctgcccggg aaagacgggg tgaatcccgt ccttgatttc
    10381 gcaccccaac agttcgtagc cccaaatgct gcgccgggca tcgaagatgg gctgccgggt
    10441 gaaaaacacg ctctgctctt ccggtgccgt ggtttcctcg gtcatgggaa tctctcttgt
    10501 cgtgccttgg gtgaggaaga aagggaagga aagaacgacc gcccccgaga gttttccggg
    10561 tagcatatcc accggggttc gtcaacagcc tgctgggttg ccaaagaaac cgagagtcgc
    10621 ggcttcgagc cggtttgaac gccgtggggg cgctcctgag gtccgattct ctcaaccatc
    10681 ggatttctct tgtttttttg cgtatgaacg atataacata cgctattaaa ttcgggcagg
    10741 ggccgtggtc tgtcattcag acgagaggtg cagcgaatgg acacagaagg tcgaagtc
//
LOCUS       DAOOVC010000132        10783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3942_length_10783_cov_5.954325, whole genome shotgun sequence.
ACCESSION   DAOOVC010000132 DAOOVC010000000
VERSION     DAOOVC010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3942_length_10783_cov_5.954325"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1344)
                     /locus_tag="PLM79_14135"
     CDS             complement(274..1344)
                     /locus_tag="PLM79_14135"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOV87500.1"
                     /translation="MKPQSNGEANPVKTLIVDDAPMMRKVIGQILAKDPRIQVVGTAA
                     NGQECLNKIRETKPEVITLDIDMPVMNGITTVKNIMVRHQIPVVIISSLVQDGYFAFE
                     SLRLGVVDFLPKPSRVQIGNWDEEEDLVRMRVLAASGMRVHRMRRVRRRRMDRGSCRN
                     GSSPGAVVVMGTTLAGPNTIMHIVTRMAPTFQGAIVALQEIHPKILVPFCSYFNRISP
                     LEVLPVLDSTPLEAGRVYLGSTFRGIHLEKDREATGKLVLQVDRSGEEPINGLFRSAS
                     RCLEDRVCGVLLTGVGEDGSRGMGEIREGGGLTIAQEQDCCVYPNLVEHALRENVVDL
                     VISSEGIAARLQAWVEDKCPVS"
     gene            complement(1328..2902)
                     /locus_tag="PLM79_14140"
     CDS             complement(1328..2902)
                     /locus_tag="PLM79_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87501.1"
                     /translation="METVGSSSPGFRGMVQLRLSDLIQMVCLARSDLVVQVRSGDRNG
                     SIFVKEGRIYHAETARLNGEEAFFEIFRWEDGHFEALPEGENKPPSIQKPWEHLLLEA
                     VRRRDEGSPEGKGPDPGEEAGPERALEASPGAEGVWVEPPWLEEGIDRAIDGLEAIPG
                     ASPDFKPSEAANPRELHRLQVLVVDDSAFFARQLKSLLEKDGAIEVVGVARNGRDALE
                     ILASGPYPDVVTLDAHMPVMPGDTTLKHIMIRHPVPVLILSSFEERSVESIFEFLEVG
                     AVDLFSKPRASDDGEEFGRRLREQVQRVSRARVARFRRRRKRTILQDPGVRRPSERIL
                     AILGAEGAHLEWLRLPLLSLCRQGIVFGFQRISRAFLEGFGRFIEEGCSVPTRLLEAG
                     SVSRPGTFCLGTGNEGLNPQWRPGDSSLTWGPGDETGETWSQAVGTRLAALSSLARGR
                     LDVHVLSSAEPFEPALAEALLASGTRFFLSPPDSVLCEDMVRAVEPYEAAFPNQVLFH
                     GPETLMEAWLDHETAV"
     gene            complement(2967..5753)
                     /locus_tag="PLM79_14145"
     CDS             complement(2967..5753)
                     /locus_tag="PLM79_14145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOV87502.1"
                     /translation="MSSEDFFQEYVDEVQEHLQELESSLLAIERQGTDRESIGQIFRA
                     VHSIKGASAYMGYEKLAHLTHELESRIHGIQTDARPVTPEEISVLLECVDFVGQALVG
                     LQESGAEPSVPESLLQKMRSVLAPGAERLEAGSVREVPGGTAPGTLETREPAKAPKSG
                     EPSRELATSDPFFPTLSADGTEVGPEEIFEEDREIYEIFLGTFQERLRALTGLPALSP
                     GETLSHENGEKAVDLLKEMISSSRYMDYEQVVEVLSQWETSLRGAMGKGRLDRRIFLE
                     HLESYGGKLAAMLPGLQLPGVDDADTISREEVGGTREEEDKELFSIFLDSFGQALGRL
                     AGIRPRQSETRLSPSQVEEAVEAVRRMIASARYMDYEEITGVLGEWAERLEESAKSGP
                     LPFETYGRMLDDYGRRLKRMLPGLNADPPGVGASTDSLSDEIEREIESSLNDLDRAMS
                     RDFVPGRENAVPPDARDRRGLAGDEEGGERDVSVRGPEKPVAQKERIPVFAEELTSSV
                     TLRVDAHKVDQLLNQLGELVVTRSEFIQTSASFRDMLRELAAQGKLTKQELRKFRLLS
                     FRLNESTLSLGRVANDLQASVMRIRMLPISQLFQRFPRVVRDQAMKHGKKVELFVEGG
                     ETEMDKRVLEQMNDPLVQFLRNAIAHGIEPPEERVRAGKPPTGTIRLAAYHAGDYVAL
                     EIEDDGRGIDVQKLRHILQDRRDIASQELNRLSDQELIYAIFLPGVSTRDQVDGSAGR
                     GVGLDVVKENVERMNGSIHVDSFPGRGTRFTIWIPLTVAIIRALLVKAENQIFTLPLT
                     SVSEIHRYRIEDTHTIEGFEVISLRGKTIPLVQLSRLLGMSGKDADRTRQFIVIVSTR
                     FQEVGLVVDGLMGEREVVIKSIEDGVHTFEGFSGATILGDGTVSLILDVSTLLRNMKQ
                     GIGPNAGAEEVTVH"
     gene            complement(5856..6206)
                     /locus_tag="PLM79_14150"
     CDS             complement(5856..6206)
                     /locus_tag="PLM79_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87503.1"
                     /translation="MRRVLVVDDSNLMRHRIVQCLTSAGHQVVGKAKDGNEAVDLYVR
                     VRPDVVTMDITMRGKDGITAAREILAMDPAACIVFYTLLDIPDLAAQVRRVPVRKVVR
                     KGDEADLLKTLSEL"
     gene            complement(6309..8807)
                     /locus_tag="PLM79_14155"
     CDS             complement(6309..8807)
                     /locus_tag="PLM79_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV87504.1"
                     /translation="MFGIKKVRRSLKAKILLGLLVITLIPLGALNLLNYMTLQTQLEE
                     DQASRLVGFSERIAKTIDLTLNERITDVVTWASLENIKNTIQNAEGLGTANQVLEGLA
                     KSYGTFDILQLFDRSGLCIASNYAKFTGTLMVQDQNWFRSVLEGKEYVGDFGNYPLVK
                     EMVPESNGWSMIIGAPVVIHGEVKGVLVGYLRWAAVNHIVEAFPVGQTGYTYLVDRAD
                     MTVIGHPTHEIIGLKLTDPKINVPMVAAAMSNQSRSFLLYDFQNPVTKKLVTRAVGFT
                     HNDGTGRFNKKWAVATGADADEIFAAIPKQRMRNAVISVIFITFLICGSLFISRRISK
                     PVIEASDMMLAITQSLDFTQSIRITGEDEIARMQEAFNGLVLRLRQTFGNIVEGNERV
                     SQVVGQVKEISGRIVVNASEQAQRAQDVLSRIETMGKTAGEVQQNAIESQRSYDDTVA
                     SISALTASIQEVARLAQSQAAMSEEARNIVNLMGETAQQVSGRAALQQQEAMEAAGAA
                     QQMAQSIGEVSDKASQAGKQSEMSFHAAVEGKKAVEEVVQGMQSIAESSEQITEIIEV
                     ISDIADQTNLLALNAAIEAARAGEHGRGFAVVAEEVRKLAERTAESTKEIGSLIKNSA
                     DRVREGMDLAGSSQKALTNIVAAVERSHTWIKEIDTAAAEQTKGIQHVVETMDRLRKL
                     SDEIMQMTSEQVKRRERAANITNDLYGISQQVSAATQEQVKNADQVMKDVSLATQRAE
                     NITSLTSMQKERSASLREVMQEMSTVALNNASGAKNSQQYSENLASVVVDFTRLIAQF
                     KIAQGDGNGRGKPERLVTESGSRSGETGEARLSA"
     gene            complement(8842..9423)
                     /locus_tag="PLM79_14160"
     CDS             complement(8842..9423)
                     /locus_tag="PLM79_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOV87505.1"
                     /translation="MGLPSVLSKVPTQTQEETKAFVQLVGFRLGDEEFGVDILMVQEI
                     IRLPNITPLPNAPGFILGMINLRGKIIPIIDLRRRLRIRGTNLEVNDRKTRILIVEIF
                     NHVTGFIVDSVSEVMKVRTNEIEPTPHLVATSIDAEYIRGVIKLPNRLVMLLDFRQIL
                     KPHEGQQLESVYSDAPEEGPRYGEPNQVVSQGI"
     gene            complement(9466..>10783)
                     /locus_tag="PLM79_14165"
     CDS             complement(9466..>10783)
                     /locus_tag="PLM79_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOV87506.1"
                     /translation="ALDDAAAPIYPAQRELYKLIDGRTPAGAIIQASPLSRPRTLSCL
                     AKWLSMRDIEEEPREPVPSTCRVLIVDDSPLMCGALQSIFSRDSRLEIVGCAHDGIEA
                     LELIDRQKPDVVTLDLQMPRMDGLTTLKHIMIRNPTPVVVLSAFTQETSRWTYESFKY
                     GAVDVMPKPGRPETGETTTEMGLSDRVSQASRVRLQAARYIRKNKCVGADSEDAATAS
                     TKSFAESPLRECRILIVCGTGGFPSLLKILLSVSRVKGLAPMIGCVGMPPRVVSALLP
                     NLEQDMGTKIEILGDPSSCRPGTVYLCSVEDPAGQWGHASPELRPGGTEGGGNEGRPL
                     DRLLGSLARKFGPRLLTILISGTGEDGIRGMESVKQAEGETYVLSPEACLRPDLPRKI
                     LDLGLAREVASTDRAVEVLRKWNDRMMAAAAVDGGLERIARVDPTT"
BASE COUNT         1899 a         3414 c         3200 g         2270 t
ORIGIN      
        1 cgagtcccga ggggtcaagt ctccgtatgg ctttttccgt gcgtgtagag ggtgtagcgg
       61 cgaccggccg gtcgccgctt ccaggcgcgc gcaaatttac ccttgttcac gggacgtttc
      121 cgcgtcccca ggggtcgact tcctcttggg acctgttccg gggggatcgg ccttgggggc
      181 ctcgattccc ggcgaatggt tgcggaccct ccggggtcgt tcggagggga cgatccatcc
      241 cctcctgatc gcagatgcgg gtaaagggga ggcctaggaa acgggacact tgtcctcgac
      301 ccatgcctgc aggcgggcgg caatgccttc gctcgagatg accaggtcta cgacgttttc
      361 tcggagagca tgttccacga ggttcggata cacgcagcag tcctgttcct gggcgatggt
      421 gaggcctccc ccctcccgga tttctcccat gcctcgggac ccgtcttccc ccaccccggt
      481 caaaagcact ccgcagactc gatcctcgag gcaccgggaa gcgctccgga acagtccgtt
      541 gatgggctct tcccccgacc ggtccacctg caatacaagc ttgcccgttg cttcccggtc
      601 tttctcgagg tggatcccgc ggaaggtgga gcccaggtag actcttccgg cctcgagggg
      661 ggtggaatcg agcacgggga gcacctcgag gggactgatc cggttgaagt aagaacagaa
      721 gggaacgagg atcttggggt ggatttcctg gagggccacg atggcgccct ggaaagtggg
      781 agccattcgc gtcacgatgt gcatgatggt gttgggcccg gccagggtgg ttcccatgac
      841 caccacggct cccgggctgc ttccgttccg gcacgagccg cgatccatcc tgcgccgacg
      901 caccctgcgc atgcggtgca cccgcatccc cgaggctgcg agaacgcgca ttcgcaccag
      961 gtcttcctcc tcgtcccaat tgccgatctg cacccgggac ggcttgggca ggaagtccac
     1021 cacgcccagg cgaagggact cgaaggcgaa gtagccgtcc tggaccaggg aactgatgat
     1081 cactacggga atctggtgcc tgaccatgat gttcttgacg gtggtgatgc cgttcatgac
     1141 gggcatgtcg atatcgaggg tgatcacctc gggtttcgtc tcccggattt tattgagaca
     1201 ttcctgcccg ttggcggcgg tgcccaccac ctgaatccgg gggtccttgg ccaggatttg
     1261 cccaatgacc ttgcgcatca tgggcgcatc atccacgatc agggtcttaa cgggattcgc
     1321 ctccccatta gactgcggtt tcatgatcca gccatgcctc catgagggtc tcgggtccgt
     1381 gaaacaggac ctggttggga aacgccgcct cgtacggttc aacggcccgg accatgtctt
     1441 cgcaaaggac cgaatccggc ggactcagga agaaacgggt acccgaagcc aggagggctt
     1501 ccgccagggc gggttcgaac ggttccgccg acgagagcac gtgtacgtcc aggcggcccc
     1561 gggccaggga agatagggcc gccaaccggg tcccgacggc ctgggaccag gtctcccccg
     1621 tctcgtcccc ggggccccag gtcagggaag aatccccggg gcgccattgg gggttcagtc
     1681 cctcgtttcc cgttcccagg cagaaggtgc ccggtcggga gacgctcccc gcctcgagca
     1741 gccgggtggg cacggaacat ccctcctcga tgaagcgacc gaacccctcg aggaaggccc
     1801 gggagattct ctggaaccca aagacaatcc cctgtctgca caaggaaagg aggggcagcc
     1861 gcagccactc gagatgggcc ccttccgccc caaggatcgc caagatccgc tccgacggcc
     1921 tacgcacccc agggtcctgc aggattgtcc tttttcgccg tcgtctgaaa cgagccaccc
     1981 tggctcggga cacccgctgc acctgttcgc gcagtctccg tccgaactct tccccgtcgt
     2041 ccgaggcccg gggtttggaa aagaggtcca ccgcccccac ctcgaggaat tcgaagatgc
     2101 tttccacgga acgttcctcg aagctgctca ggatgaggac gggaacggga tggcgaatca
     2161 tgatgtgctt gagggtcgtg tctcccggca tgacgggcat gtgggcatcc agggtgacga
     2221 catccgggta ggggcccgag gccaggattt cgagggcgtc tcgaccattc ctcgccaccc
     2281 ccacgacctc tatggcgccg tccttctcga ggagactctt gagctgtcgg gcgaaaaagg
     2341 ccgaatcgtc caccaccagg acttgaagcc tgtgcaactc cctggggttt gccgcctcgg
     2401 aaggcttgaa atcaggggag gcgccgggaa tggcctcgag gccgtcgatg gcccgatcga
     2461 taccctcctc gagccacggg ggctcgaccc aaaccccctc ggccccgggg gaggcctcga
     2521 gggccctttc cggtcccgct tcttccccag ggtccggtcc cttcccttcc ggcgatcctt
     2581 cgtctcggcg acggaccgcc tccaggagga gatgttccca gggcttttgg atggaggggg
     2641 gtttgttttc cccctcgggg agggcctcga agtggccgtc ctcccagcgg aagatttcga
     2701 aaaaggcctc ttctccgttc aatcgtgccg tttccgcatg gtagatccga ccttccttga
     2761 cgaaaatgga accgtttcga tcccccgacc gcacttgcac cacgaggtcg gacctagcca
     2821 ggcagaccat ctgaatgagg tccgacaacc gcaactgcac catgccccgg aatcccggag
     2881 aagaagatcc gaccgtctcc atggtttcca cttcttgagg gtccatccac aagggagagt
     2941 cccccctcga tctcagcccg ggtccgtcaa tgaacggtaa cctcttccgc gccggcattg
     3001 gggccgatgc cttgcttcat gtttcgcagc aaggtggata catccaggat cagggagacg
     3061 gtaccgtccc ccaggatggt ggcccccgaa aagccttcga aggtgtgaac cccgtcctcg
     3121 atgctcttga tcaccacttc ccgctccccc atgagtccgt ccaccacgag ccccacctct
     3181 tggaatcggg tgctgacaat gacgatgaac tgccgggtcc ggtcggcatc ttttccggac
     3241 atgcccagaa gtcggctcaa ttgaaccagg ggaatggtct ttcctcggag gctgatgacc
     3301 tcgaacccct cgatggtgtg cgtatcctcg atccgatagc ggtggatttc ggagaccgaa
     3361 gtgaggggta gagtgaagat ctggttctcg gcttttacca acagggccct gatgatggcc
     3421 accgtcaggg gaatccagat ggtgaaccgg gtgccccggc cgggaaagga atccacgtgg
     3481 atactgccgt tcatgcgttc gacgttttct ttgaccacgt cgagtcccac gcctcggccc
     3541 gcggacccgt ccacctggtc ccgggtggaa acccccggca ggaagatggc gtaaatgagc
     3601 tcctggtcgg acagccggtt cagttcctgg gaagcgatgt cccggcgatc ctggagaatg
     3661 tggcgcaatt tctgaacgtc gatgccccgg ccgtcgtcct cgatttcgag ggcgacatag
     3721 tctcccgcat ggtatgctgc aaggcggatg gtgcctgtgg gaggcttacc tgcacgaacc
     3781 ctctcctcgg gaggttcgat cccgtgggca atggcatttc gaagaaactg caccagggga
     3841 tcgttcatct gttcgagcac ccgtttgtcc atctccgtct cgccgccttc cacgaacagt
     3901 tcgacctttt ttccgtgttt catggcctgg tcccgaacca cccggggaaa ccgctggaac
     3961 agctgggaga tcggcagcat ccggatgcgc atgacggaag cctgcaggtc gttggccacc
     4021 ctccccaggg acagggtgga ttcgttgagg cgaaagctca ggaggcggaa ctttcgcagc
     4081 tcctgcttgg tcagcttgcc ctgggccgcc agctcccgga gcatgtctcg gaaggaagcc
     4141 gacgtctgga tgaattccga ccgggtgacc accagttcgc ccagctggtt cagcaactga
     4201 tccactttgt gggcatccac ccgcagggtg accgacgagg tcaactcctc ggcaaagacg
     4261 ggaatccgtt ctttctgagc taccggtttt tcgggacccc ggacggacac atccctctcc
     4321 ccgccttcct cgtctcctgc gagtcctctt cgatcccggg cgtccggggg cacggcattc
     4381 tcccgaccgg gcacgaagtc ccgggacatg gcccggtcca ggtcattgag ggaggattcg
     4441 atttcccttt cgatctcatc cgagagggaa tccgtggaag cgcctacacc gggcgggtcc
     4501 gcattcaggc ccggcagcat gcgtttcaat cgccggccgt agtcatcgag catccgccca
     4561 taggtttcga aaggcaaagg gccggatttg gcggattctt cgagccgttc cgcccattcg
     4621 cccaggactc cggtgatttc ctcgtaatcc atgtaacggg ccgaagcgat catccggcgg
     4681 acggcctcga cggcctcctc gacttgggaa gggctcagcc gggtctcgga ctgccgggga
     4741 cggatccctg cgagtcgacc cagggcttgt ccaaaggagt cgagaaaaat cgaaaaaagc
     4801 tccttgtcct cttcctcccg ggtccctccc acctcctccc gggagatcgt gtccgcgtcg
     4861 tcgacgccgg gcagctgcaa ccccggcaac attgccgcca gtttgcctcc ataggattcc
     4921 aggtgttcca gaaaaatccg tcggtccaga cggccttttc ccatggcccc tcttagagaa
     4981 gtttcccatt ggctcagcac ttccactacc tgctcgtagt ccatgtaccg ggaagagctg
     5041 atcatctctt tgaggagatc gacggccttt tccccgtttt catgggacag ggtttctccc
     5101 ggagaaaggg ccgggagtcc ggtcagggcc ctcaaccgtt cctgaaaggt gccgagaaag
     5161 atttcataga tttcccggtc ctcctcgaag atttcttccg gccccacctc ggtaccgtcg
     5221 gcgctcaggg ttggaaagaa ggggtcgctt gtcgcgagtt ccctcgaggg ttccccggat
     5281 ttcggcgcct tcgcgggctc ccgggtctcg agggtccccg gcgcggtccc gcccggcact
     5341 tcccggaccg atcccgcctc caacctctcc gcccccggcg ccagtacgga ccgcatcttt
     5401 tgaagaaggg attccggcac cgaaggctcc gcaccggatt cctggagacc caccagggcc
     5461 tggcctacaa agtccacgca ttcgaggagc acgctaattt cttccggcgt gacgggccgg
     5521 gcatcggttt gaatcccgtg gattcggctc tccagctcgt gggtcaggtg agccagtttt
     5581 tcatagccca tataggccga ggcgcccttg atgctgtgga cggctcgaaa aatctgtccg
     5641 atggattccc ggtccgtacc ctgccgctcg attgccagca gagagctctc cagttcctgg
     5701 agatgctcct gcacctcatc tacgtattcc tggaagaagt cctccgacga catatgaccc
     5761 tctctttgtc tatctcccaa acggggcgat cggtcccggc gtgttgacgg aaggccaatc
     5821 gcccattccg ggtcctttcc ccggcggatc gttccctaga gttccgaaag ggtcttgagg
     5881 aggtcggcct cgtccccctt ccggaccacc ttcctcaccg ggacccttct cacctgcgcc
     5941 gccaggtcgg ggatgtccag cagggtgtaa aagacgatgc aggccgccgg gtccatggcg
     6001 aggatttccc gggcagcggt gatgccgtct tttccccgca tggtgatgtc catggtcacc
     6061 acatcgggtc gcacccgcac ataaagatcc acggcctcgt tgccgtcttt ggcctttccc
     6121 accacttggt gtccggccga ggtcagacac tggacgatgc ggtgccgcat caggtttgaa
     6181 tcatccacaa ccaaaactcg tctcatgata cgggccattc cttggattgg tgcgttcaca
     6241 tcgttgctta ggcctttccc cggatgggcg cttgcccctc ccccgcctcg aactccctta
     6301 tcgaggaatc acgcggagag ccgcgcctcg cccgtctccc ccgaacggga accggattcc
     6361 gtgacaagcc gctccggttt gccccgcccg ttgccgtctc cctgggcgat cttgaattga
     6421 gcgatcagcc gggtaaagtc cacgaccacg ctggccaggt tctcgctgta ctgctgggag
     6481 ttcttggccc cggatgcgtt gtttagagcc acggtgctca tctcctgcat gacttcccgc
     6541 agggacgcac ttcgctcctt ttgcatgctg gtcaacgacg tgatgttctc ggcacgctgg
     6601 gtggctaagg acacgtcctt catgacctgg tccgcattct tcacctgttc ctgggtggcg
     6661 gcggagacct gttgcgagat cccgtagagg tcgttggtga tgttggcggc ccgttcgcgc
     6721 cgtttcacct gttcggaggt catctgcatg atttcgtcgg acagtttccg caggcggtcc
     6781 atggtctcca ccacgtgttg aatgccctta gtctgttctg cggcggcggt gtcgatctcc
     6841 ttgatccagg tatggctccg ttcgacggcg gcgacgatgt tcgtcagagc cttctgactg
     6901 gagccggcca ggtccattcc ttccctgacc ctatcggcgc tgttcttgat gagtgagccg
     6961 atttccttgg tggattcggc ggttcgttcg gcaagtttgc gaacttcctc ggccaccacc
     7021 gcaaacccgc ggccgtgttc cccggcccgg gccgcttcga tggcggcgtt cagggccagc
     7081 aggttggtct gatccgcgat gtcggagatc acctcgatga tttccgtgat ctgctccgag
     7141 ctctcggcaa tgctttgcat gccctgcacc acctcttcca ccgccttttt cccttccacg
     7201 gccgcgtgga aagacatctc cgactgctta cccgcctggg aagctttgtc cgaaacctcc
     7261 ccaatggatt gcgccatctg ttgggccgcc ccggccgctt ccatggcctc ctgctgctgg
     7321 agggcggcac gccccgatac ctgttgcgcc gtctccccca tgaggttgac gatattcctg
     7381 gcttcctcgc tcatggccgc ctgggactgg gccaaacggg caacctcctg gatgctcgcc
     7441 gtgagcgccg aaatcgaggc caccgtgtcg tcgtaggatc gctggctctc gatggcattc
     7501 tgctggactt ctcccgcggt ctttcccatg gtctcgatgc ggctcaacac gtcctgggcc
     7561 ctctgagcct gttccgaagc gttcaccaca atgcgcccgg aaatctcttt cacctgtccc
     7621 accacctggg acaccctctc gttgccttcc acgatgtttc caaaggtctg gcgcaagcgg
     7681 agcaccaggc cgttgaatgc ctcctgcatc cgcgcaattt catcctctcc cgtaatccgg
     7741 atgctctggg tgaaatccag gctctgagtg atggccagca tcatatccga agcctcgatc
     7801 accggcttgg agatgcgacg actgatgaac agagacccgc atatgaggaa agtaatgaaa
     7861 atcaccgaga tgacggcgtt gcgcattcgt tgtttgggaa ttgcggcgaa gatctcgtcg
     7921 gcatcggccc ccgtggccac cgcccatttc ttgttgaacc ggccggtccc gtcgttatgg
     7981 gtgaatccca cggcccgggt cacaagcttc ttggtcacgg gattctggaa gtcgtagagc
     8041 agaaagcttc gactttgatt ggacatggcg gcggccacca ttgggacgtt gatcttgggg
     8101 tccgtgagct tcagcccgat gatttcgtgg gtgggatgcc cgatgaccgt catgtcggcc
     8161 cggtccacca agtaggtgta cccggtttgt cccacaggga aagcctctac gatgtggttc
     8221 accgcggccc acctgaggta gcccaccagg acccccttga cctccccgtg aatcaccacc
     8281 ggcgccccta tgatcatgga ccacccgttg gactcgggaa ccatttcctt caccagggga
     8341 tagttgccga aatccccgac gtattccttc ccctcgagga cggatcggaa ccagttttga
     8401 tcctggacca tgagcgtccc ggtgaacttg gcataattgg aggcgatgca aagcccggaa
     8461 cggtcgaaga gctgcaggat gtcaaaggtc ccgtaagact tggcaagccc ctcgaggacc
     8521 tgatttgccg tccccaggcc ttctgcgttc tgaatggtgt tcttgatgtt ctcgagggac
     8581 gcccaagtga cgacgtcggt gattcgctcg ttcagggtaa gatcaatggt tttggcgatg
     8641 cgttccgaga aacccaccag acgggaggct tgatcttcct cgagctgtgt ttgcagggtc
     8701 atgtaattga gtagattcag tgcacccaga ggaatgaggg tgatcaccaa gaggccaagc
     8761 aatatcttgg ctttcaacga acggcggact tttttgattc cgaacatggc gcctgtcctc
     8821 cttcatgctc cgtcggtccc atcagatccc ctgggagacg acctggttgg gttctccgta
     8881 cctgggcccc tcctcggggg catccgaata tacgctttca agttgctgtc cttcgtgggg
     8941 ttttagaatc tgtcggaagt ccagcaacat cacgagacga ttgggcagct tgatgactcc
     9001 acggatgtac tcggcatcaa tgctcgtggc cacaagatgc ggcgtgggct cgatttcgtt
     9061 cgttctcact ttcatcactt ccgagacgga atccacgatg aagcccgtca cgtgattgaa
     9121 gatctccaca atgagaatgc gcgtcttccg atcgttgact tcgaggttgg tcccccggat
     9181 tctcagtcgc ctgcgcagat cgatgatggg gatgatcttg ccccgcaggt tgatcattcc
     9241 cagaatgaac ccgggagcgt tgggcagagg ggtaatgttc ggcaggcgaa taatctcctg
     9301 aaccatgaga atatcgaccc caaactcctc gtcccccagg cgaaaaccga ccagctggac
     9361 aaacgctttg gtctcttcct gggtctgggt cggcaccttg gaaagaactg agggcaatcc
     9421 catcgtcatc ctccttaccg caggggaccc cccctgcact tttcttcagg ttgtcgggtc
     9481 gactcttgcg atcctctcca ggccgccgtc cacggcagcg gccgccatca tccgatcgtt
     9541 ccattttcga agtacttcca ccgcccgatc ggtgctcgcg acctcccggg caagccccag
     9601 atcgaggatt ttccggggca ggtcaggtct caggcaggct tcgggcgaca ggacgtaggt
     9661 ttctccctcc gcctgcttta cggattccat gccccgaatt ccgtcctcgc ccgtaccgga
     9721 aatcagtatg gtcaaaaggc ggggtccgaa cttccgggcc agggagccga gcagtcggtc
     9781 caggggacgg ccctcgttcc cgccgccttc cgttccgccg ggacggagct ccgggcttgc
     9841 gtgcccccat tgtccggcag ggtcctcgac cgagcacaga tagacggttc ccggtcggca
     9901 ggaagacggg tccccgagga tttcgatttt ggtccccatg tcttgttcga ggttgggcag
     9961 gagggcggaa accactcgag ggggcatccc cacgcaaccg atcatgggag ccaggccttt
    10021 cacccgggac acggaaagaa gaattttcag gagggacggg aagccccccg ttccgcagac
    10081 aataaggatt ctgcactctc gcagtgggga ttctgcgaaa gacttcgttg aggccgtcgc
    10141 cgcgtcttcc gagtccgctc caacacattt gttttttcgg atgtaccggg ccgcctgcaa
    10201 ccgtacccgg gacgcctggc tcacccgatc ggaaagcccc atctccgtgg tcgtttcccc
    10261 ggtctcgggt cgccccggct tgggcatcac gtccacggcc ccatatttga aagattcgta
    10321 agtccaccgc gaggtctcct gggtgaaggc gctcaggacc acaaccgggg tgggattgcg
    10381 aatcatgatg tgcttgaggg tcgtgaggcc gtccatacgc ggcatctgga gatcgagggt
    10441 gaccacgtcg ggcttttggc ggtcgatgag ctcgagggcc tcgatgccgt cgtgagcgca
    10501 tccgacgatt tcgagacgcg agtctctgga aaagatggac tgcagggcac cgcacatgag
    10561 gggggagtcg tccacgatga gcacccggca ggtggaagga accggctccc ggggctcttc
    10621 ttcgatatct ctcatggaca gccacttggc caggcagctc agggtccggg gccgcgagag
    10681 gggcgacgct tggatgattg ccccggccgg ggtgcgcccg tcgatgagct tgtagagctc
    10741 ccgctgggcc gggtagatgg gtgcggccgc gtcgtccagg gcc
//
LOCUS       DAOOVC010000133        10766 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3947_length_10766_cov_7.664270, whole genome shotgun sequence.
ACCESSION   DAOOVC010000133 DAOOVC010000000
VERSION     DAOOVC010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10766
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3947_length_10766_cov_7.664270"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..991)
                     /gene="meaB"
                     /locus_tag="PLM79_14170"
     CDS             complement(53..991)
                     /gene="meaB"
                     /locus_tag="PLM79_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOV87507.1"
                     /translation="METHYAEQVLGGSIRTLARLISWLEDEDERAFPVLEKLYPHTGK
                     AYVVGITGSHGTGKSTLIGRLTRVIREQGLTVGIIAVDPSSPFTGGALLGDRVRMSDL
                     FNDPGVYICSMATRGFLGGMAGATAEAVKVLDAFGKELILIETVGVGQDEVDIINIAD
                     TVCLLLSPGLGDAIQVMKAGVMEIADVFVVNKADRPGTDGLCREVQFSLAHRRRGAED
                     WIPPVLRTVAVNGVGIRQLWKDIQDHRQYQETSGRLYERRKERLCRDMLRMIHEEIFR
                     RICRSLSENGQLDELVREITENRLDPRRAMRRLTGC"
     gene            1531..2385
                     /locus_tag="PLM79_14175"
     CDS             1531..2385
                     /locus_tag="PLM79_14175"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV87508.1"
                     /translation="MIIPNKQEILQQISRVKDLPPLPSSLERLLAVVQSQVTTPEELE
                     NIIHYDQSLTARVLRVANGAYYGNRGKVTTISRAIALMGFDQVKSVCLLALLLQAFEH
                     GPTLSAAERESLWKHAFCTARLAGRMVERRPWATREKAYLFGLLHDLGKLVMLIYFQD
                     YYLALQDLAREKKVSFRCMELQFGLTHTRLGRWVAVKWGFPEVFQNVMEFHHTPWESS
                     SHRPEVKLIALADILANSRNFPDPASHEIAADCCRDLHVGEDEWEDYRSAVPEVWLEV
                     EHLWRLLS"
     gene            2396..4501
                     /locus_tag="PLM79_14180"
     CDS             2396..4501
                     /locus_tag="PLM79_14180"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87509.1"
                     /translation="MDQLMQEAVRMGLVGAQAQSEEATLFLGMIQRFVELLAQKKGRT
                     NLCKELVRIVIEETSFDNCSIVLWNAQTKNLVLEAAYGLEDLLGTDSASAYHRDLAFP
                     VGEGIAGRVFAEKTPIFVDESLPASIPPVEDAVIHPASLACLPLLDLGVLCLSASQPD
                     RFSDALKRNWEHLSKIMGYLILGASLLPDDSPKTRCPSPLEANGTTRSPDPLSSFSSN
                     LSEQALNRTPQGICILDPLGNILRVNQTIETIQGGTGSDLIGRSPAVLFRDPRIFRTL
                     FEDVKRYDRQERNDVSLVSSRGAVYQADVFLTGLHQDGKVVAYLLVINDITQKKAFAE
                     KLVQTEKLAALGTMAGGVAHDFNNLLMAILGNIQLVMPSVGDEEIRRRLQNIEKAVHD
                     GAHTVRRLQKLTEKDRDSQAAAIPADLVEVVSDVVELTRPRWKDAMERHGSSIAFKLE
                     LEPDCLAAIHPSDLREILTNLIFNAIEAMPEGGTITFRSRSFQKHVVLEIADTGIGMG
                     EEIQSKIFDPFYTTKGIGNSGLGLSVCWSLANRAGGDISVKSRPGKGTVFFIQLPKAP
                     PPTRTSESRRVGDEKRACRILLVEDDPQIMGILRDMLRLKGHRVTATENSEKALILIE
                     EEDFDLVLTDLGMPVVSGWDIAKRAKSKSPEVPVVMITGWGAQYEEEDLTGRGVDLLL
                     SKPLSWDKLLKSIEKLLHR"
     gene            complement(4526..5635)
                     /gene="dnaN"
                     /locus_tag="PLM79_14185"
     CDS             complement(4526..5635)
                     /gene="dnaN"
                     /locus_tag="PLM79_14185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOV87510.1"
                     /translation="MKFQVDKGPLIQVLQRVQGITEKKTSMPILSNCLLRASETNLLE
                     FSATDLELSIWTQTEGKVESPGATTISARKLLEIVREIPQESILMESLPNQRLCLSAA
                     RAQFELAILAAEDFPHMALPQDAPLVSADLSAFKQALQKTLYAISPEDDPFSVAGVYC
                     QPVGTSDLRFVSSDGHRLAYVQVPRESLGNLEVGPGIIIPRKGIQEILRILERETEVS
                     LGIQESSFLLKTPHSFLSIRMLDADYPDYEQIIPRERPFSLEISWENLHSALKRMAVF
                     TNSKWRYVKFMVSNGTLALQAGNPELGNANDVLDVEYQGEEFSIALNVRYVLEAIQVM
                     ESREVRFEWLDAYHGAVFLGADDPGYLGLIMPMVV"
     gene            6334..8097
                     /locus_tag="PLM79_14190"
     CDS             6334..8097
                     /locus_tag="PLM79_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOV87511.1"
                     /translation="MAYDAVRMKDWQISEASEKNMPTPSDWQDRLSLQKDEIIPMGRL
                     CKLDFMKIIERLKDRPDGKYIEVTAITPTPLGEGKSTTSVGLMEGLGKRGKNVGGCLR
                     QPSGGPTMNIKGTAAGGGNALLIPMTEFSMGLTGDINDIMNAHNLAMVALTARMQHER
                     NYNDEQLQRLTRMRRLDVDPTRVEMGWVLDFCAQSLRNIIIGIGGRQDGFMMQSKFGI
                     AVSSELMAILSIVRDLPDLKKRMNEITVAFDKRGKAITTGDLEVGNAMTAWMRNTINP
                     TLMCTAEYQPCMVHAGPFANIAVGQSSIIADRIGLKMFDYHVTESGFAADIGFEKFWN
                     VKCRNSGLIPHVSVLTTTIRALKMHGGGPKVVAGLPLPEAYTKEDLGLLEKGVPNMVH
                     HINTIRKAGINPVVCINAFHTDTKDEIALVRKYAEAAGARCAVSEHWAKGGDGALEFA
                     DAVVDACNEKNEFKFLYPLEMKLRDRVAMIAREVYGADGVSWTPEAEAKAKLLESDAK
                     YDDFSTMMVKTHLSLTHDPTRKGVPKGWTLPIRDVLIYSGAKFLCPCAGTISLMPGTS
                     SDPAFRRVDVDVNTGKVTGLF"
     gene            8185..9075
                     /locus_tag="PLM79_14195"
     CDS             8185..9075
                     /locus_tag="PLM79_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HOV87512.1"
                     /translation="MPAKLLKGAEVAKEIREELKAEVRQLQSKHSVVPGLVTILVGEN
                     PASQSYVRAKQKTAHELGFYSVQDNQPADLSEEALLGLIDKYNRDPKIDGILVQLPLP
                     KHVNENRVLLSIDPGKDVDAFHPVNVGKLMIGEPDYLPCTPAGIQELLVRSGTEISGA
                     EVVVVGRSNIVGKPIAVMLMQKSRGANATVTVCHTRTRDVKYHTRRADILIAAAGVTE
                     YVKGDMIKPGAVVIDVGVNEVGKTPDGKRILKGDVAFDEAVEIASAITPVPGGVGPMT
                     ITMLMKNTVRACKVHNKIKD"
     gene            9299..>10766
                     /locus_tag="PLM79_14200"
     CDS             9299..>10766
                     /locus_tag="PLM79_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87513.1"
                     /translation="MIQRPWKLLLIDDDPGIRKVMTISLEDAGYTVWTAPDGESGIRA
                     FEEKSPDIVITDIRMPGMNGIEVLRRIKELDPDKEVIVATAFGDLDLAVEALQLDASD
                     FITKPIHEDALFVALKRAQERYSTRKELRDYTSLIEERWMQTAEELAKTFHFQKMLID
                     SSIDGIVACDPAGKVMVFNKSMEQMLGYPAKEAVGKMLLDDFFWSGEDKKFHHKLASE
                     EFGGKDRLYLYESTLRDRTGHKIPVQLSASVLFHEGRVAGIVGFFRDLREIRRLTQEF
                     ADQARLLHQDKMISLGKLAASVVHEINNPLAGILNYTRLMIKMLSRGPLSPESAERFA
                     GYLQLMESELSRCSKIVSNLLAFSRKSTLEFREVDVNELVLKCVLLSEHQLTLQNIRV
                     ETRLSEGLPSVQGDFNQLQQCLFNLILNSIDAMPQGGSLTLRTYREDPKGRVVMEVRD
                     TGCGIAEEDLPYIFDPFFTTKTEGKGLGLGLSTVYGIVD"
BASE COUNT         2415 a         3094 c         3022 g         2235 t
ORIGIN      
        1 gagatgattc tacatcaaac ctgaagataa tgcacgcata tttgttgctt tttcaacacc
       61 ctgttaagcg gcgcatcgcg cgccgtgggt ccagcctatt ctcggtgatc tcccgcacca
      121 actcatccaa ctgcccattc tcactcaggg accggcagat acgtcgaaag atctcctcgt
      181 gaatcatccg aagcatatcg cggcacaagc gctcctttct ccgctcatag agccgtccac
      241 tcgtttcctg gtactgccgg tggtcttgga tatccttcca caattgcctg atccccacac
      301 cgttgacggc aaccgtccgg aggaccggtg gaatccagtc ttccgcccct cttcgccggt
      361 gcgccaggct gaactgaacc tccctgcaga ggccatccgt tcccggacga tccgccttgt
      421 tgaccacgaa aacatcggcg atttccatga ctcccgcttt catgacctga atggcatcac
      481 ccagtccggg agagaggagt agacataccg tatccgcgat gttgatgata tcgacctcat
      541 cctggcccac ccccacggtt tcaataagga tcaactcctt gccgaaggcg tccaggacct
      601 tgaccgcctc cgcagtggct ccggccatgc ccccgaggaa tcctcgggtc gccatgctgc
      661 aaatgtatac acccggatcg ttgaagaggt cgctcattcg tactcggtcc ccgagaaggg
      721 ctcctccggt aaaggggctg gaaggatcta cggctatgat tccgaccgtg agcccctgtt
      781 cccgtatcac ccgtgtcagt ctgccgatga gggtgctttt gccggttcca tgcgagcccg
      841 tgattccgac cacataagcc ttgccagtgt ggggataaag cttttccaac actggaaagg
      901 ctcgttcgtc ttcatcctcc agccagctga tcagacgggc aagcgtccgg atggaacccc
      961 caaggacctg ctctgcataa tgcgtctcca tgggaatcgt cctttctttt gatcggctcg
     1021 ggaaccaacg cgctcagcct gcggtatttg tacggctgca aatatcagcc catgactcgg
     1081 caaaatcgtc aattgagggt gaattttttt atcgacgttg agaattccct atggtatggt
     1141 ggcggcgatt attgctcccc taaaatctat catcattttt ttgtggtcaa ggtccgtgtt
     1201 ggaaactggt gctgctgttt gggctccttt gtgctctgcg ggaatttttc ggcagcgggg
     1261 gagtacgcct taaggtacga ataatccttg gtttcaaaag gccatggaaa tctttgtccg
     1321 ttcactccca tttccaagca tccctcagag attgtgctct cgggggccgg atttctcccg
     1381 aaaccttgtc cctgttctta aagcgttgtc attggctcga cggggaacgg ccctcccgtt
     1441 ttgttgtcac gccgatgaaa ttcgatccat ccatagaatc tatgagaacg cagagaggcc
     1501 gcgaccgcac gcaaaggggt gagaatcacc ttgataatcc cgaataagca agaaatcctc
     1561 caacaaatct cccgggtgaa agaccttcca cctttgcctt cctccctcga acggctgctg
     1621 gcggtcgtcc aatcccaggt gaccacaccg gaagagctcg aaaacatcat tcactacgat
     1681 caatccctga ctgcaagagt cctccgggtg gccaacgggg cttactacgg gaaccgggga
     1741 aaggtaacca cgatttcccg ggccattgcc ctcatgggat tcgaccaagt gaagtccgtt
     1801 tgtctgctgg ctttgctcct tcaggccttc gagcatggtc cgacgctttc ggcagccgag
     1861 cgggaaagct tgtggaagca tgccttctgc acggcaaggc ttgccggcag aatggtggag
     1921 aggagaccgt gggctacccg ggaaaaagcc tacttgttcg gcctactgca tgatttggga
     1981 aagctcgtga tgttgatcta ttttcaagac tattacctgg ctctccaaga tctggcccgg
     2041 gaaaaaaagg tttccttccg ttgcatggag ctgcaattcg gcctgaccca cacgcggctg
     2101 ggaagatggg ttgccgtgaa atggggtttc ccggaagtgt tccaaaacgt catggagttc
     2161 catcataccc cctgggagag ttcctcccac cggcctgaag tcaagctcat cgccctggcc
     2221 gacattctgg ccaactctcg gaacttcccc gatccggcct cccatgaaat cgccgccgat
     2281 tgttgcaggg acctgcatgt aggggaggac gaatgggagg attatcgatc cgcggtgccg
     2341 gaggtgtggt tggaggtcga gcatttgtgg aggttgctga gttagaggaa ccaacatgga
     2401 ccagctgatg caagaagcgg tacgaatggg cctggtgggc gcccaggccc aatccgaaga
     2461 agccaccctg ttcctcggga tgattcagag atttgtggag ctcctggccc agaagaaggg
     2521 cagaacgaac ctctgtaagg aactggttcg aatcgtcatc gaggaaacca gctttgacaa
     2581 ctgctccatt gttctgtgga atgcccaaac caagaacctg gtcctcgagg ccgcctacgg
     2641 tcttgaagat ctcctgggca cggattcggc ctcggcctac caccgggacc ttgcgttccc
     2701 ggtaggggaa ggcattgcgg gacgggtttt tgccgaaaaa acgcccatct ttgtggacga
     2761 gtccctcccg gcttccatcc ctcccgtgga ggatgcggtg atccatcctg cctctctggc
     2821 ctgtctgcct ttgctggacc tgggggtcct gtgtctgagc gcctctcagc ccgatcgatt
     2881 ctccgacgcc ctcaaaagga attgggaaca cctcagcaag atcatgggtt atctcattct
     2941 gggagcatcc ctattgccgg atgattcgcc gaaaacccgg tgcccgtcgc ccctggaagc
     3001 aaacgggaca acccgctccc cggatcccct gtcgtccttc tcgtccaatc tgtcggaaca
     3061 ggccctgaac cgcacgcctc agggcatctg catcctcgat cccctgggaa acatcctccg
     3121 ggtcaaccaa accatcgaaa cgattcaggg aggcaccgga agcgacctga taggccgctc
     3181 tcctgcggtt cttttccgag acccccgaat atttcgaacc cttttcgaag acgtgaagcg
     3241 ctacgatcgc caggagagga acgacgtctc ccttgtgagc tcccgggggg cggtctatca
     3301 ggcagatgtc tttctcaccg gcctgcacca ggacgggaaa gtcgtcgcct acctgttggt
     3361 catcaacgac atcacccaga aaaaggcctt cgcggagaag ctggtgcaga cggaaaagct
     3421 ggccgccctg ggcacaatgg ccggtggagt ggcccacgac ttcaacaacc ttctcatggc
     3481 gatcttggga aacatccaac tggtcatgcc cagcgtgggg gacgaggaaa tccgccggcg
     3541 gctccaaaac atcgagaagg ccgttcacga cggggcccac accgttcgaa ggctccagaa
     3601 gctcacggaa aaggaccgtg attcccaggc cgctgccatt cccgcagacc tggtggaagt
     3661 cgtgtcggac gtggtggagc tcacgcggcc ccgctggaaa gatgccatgg aaaggcacgg
     3721 gagctccatc gccttcaagt tggagctcga gcccgattgc ctggccgcca tccacccctc
     3781 ggatttgagg gagatcctca ccaacttgat tttcaacgcc atcgaggcca tgcccgaggg
     3841 gggcaccatt accttccgga gccggtcttt tcagaaacac gtggtcctgg agattgccga
     3901 tacgggcatc ggcatgggag aagagatcca aagcaagata tttgatccct tctataccac
     3961 caagggcatc ggcaattcgg ggctgggact cagcgtatgc tggagccttg cgaaccgggc
     4021 cgggggagac atttccgtga aaagcaggcc cggaaaagga acggttttct ttatccagct
     4081 tcccaaggcc cctcctccca cccggacctc cgaatcgaga agggtaggcg acgagaagcg
     4141 tgcctgccgg atcctgctcg tggaagacga tccgcagatc atgggaattc tcagggacat
     4201 gctccgcctc aaaggtcaca gggtcacggc aacggaaaac agcgaaaagg ccttgattct
     4261 tatcgaagag gaagatttcg acctggtcct gaccgacctg ggaatgccgg tggtcagcgg
     4321 atgggacatt gccaaaaggg ccaagtccaa aagccccgag gttcccgtgg tcatgatcac
     4381 gggatggggg gcccagtatg aagaagagga cttgaccggg cgcggcgtgg atttactcct
     4441 gtccaagccc ctcagctggg acaagctgct caagagtatc gaaaagctgc tccaccgcta
     4501 gagccgccct tgattggtgt tcctcttaga cgaccatggg catgatcagg cccaggtaac
     4561 cgggatcgtc cgctcccagg aacacggccc cgtggtaagc atccagccat tcaaaacgaa
     4621 cctcgcggct ctccatgact tgaatggcct ccaggacgta gcggacattc aacgcaatgg
     4681 aaaactcctc cccttgatac tccacgtcca ggacgtcgtt ggcatttccc agttcagggt
     4741 ttccggcctg caaggcaagg gtgccgttgg aaaccatgaa cttcacatat ctccacttgc
     4801 tgttcgtgaa cacggccatc cgtttcaagg cggaatggag attttcccag cttatctcca
     4861 gggaaaaagg cctttcccgg ggaatgattt gctcgtagtc cggatagtcg gcatccagca
     4921 tccgaatgct gagaaaactg tggggcgtct tcaagagaaa agagctctcc tgaatcccca
     4981 gggaaacctc ggtttctctt tccaggatcc ggaggatttc ttgaatccct ttgcgcggga
     5041 tgatgattcc cggaccgacc tccagatttc ccaaggattc ccggggaacc tggacataag
     5101 ccaaccggtg gccgtctgag gaaacgaagc gaaggtcgga agttcccaca ggctgacaat
     5161 aaacgcccgc cacgctgaag ggatcatcct cgggggaaat tgcgtagagg gtcttctgca
     5221 aagcctgttt gaatgccgag agatcggcgg aaaccagagg ggcgtcctgc ggaagagcca
     5281 tatgcggaaa atcttcggcg gccaggattg ccaattcgaa ctgcgccctt gccgccgaca
     5341 ggcaaagccg ctggttggga aggctttcca tgaggatcga ttcttgcggg atctcccgga
     5401 cgatttccag gagcttcctg gcggaaatgg ttgtggcccc cggagattcc acttttccct
     5461 cggtctgcgt ccaaatactc aattcgaggt ccgttgcgga aaattccaac aggttggttt
     5521 cggaggcccg cagaaggcag ttggacagga tcggcatgct ggtcttcttt tccgtgatgc
     5581 cctggacgcg ctgaagtacc tggatgaggg ggcccttgtc gacttggaat ttcatggttc
     5641 gtcctcggtt atggttgtaa aaatttagac ttacaaaggt tcatcatctt aatagggggg
     5701 tgttcacatg tcaaaaattt tgtcaggttt ttgattttac tgtttatact tgtgaacagc
     5761 ctcccgacat cgggccgccg gttctttccg atttcttccc ggtcccggac gcttccccga
     5821 cttgcacaca ggttgtgaac accgtgtgaa catatttcca taggtttttt aatactaaac
     5881 atgtttaatt catatcaaat gtatccagct gaagtaagga ctggtagatg attttgccat
     5941 aaatcagata gcagaacctt tttctccctt gacaaaagga ccctcctcca gtaggtttta
     6001 gcgtcgttta tatgcagaga agtggtcgct ctgcgggttc tgggtgaata gcatttgttc
     6061 atgtgtagat tgggcgaggc gagaggaacc gaagtcttct agcctaagat actgagtttg
     6121 tcacgtccct aattcacatc cccattcacc gttacaaaag atccgcgccc gcgtctgtcc
     6181 tatgcccgat aacgagccga atcctacaag aaggaggtgt gtggagaaat cttgaaaaat
     6241 tttgccctgt tgtttcgttc atggcccgaa caatagactg gaaagcgttc acaaggatcc
     6301 aggaaagctt ctacccgagg gaggagaaac gaaatggcct atgatgccgt aagaatgaag
     6361 gactggcaga tttccgaagc atccgaaaag aacatgccca ccccgtccga ctggcaggat
     6421 agactgtctt tgcagaagga tgaaatcatc cccatgggga gactctgcaa gttggatttc
     6481 atgaagatca tcgagcgcct caaggaccgg cccgatggca aatatatcga ggtgacggcc
     6541 atcaccccga cccctttggg agagggaaag agcactacgt ccgtgggtct catggaaggg
     6601 ttgggaaagc ggggcaagaa tgtgggcggt tgtctccgtc agccgtcggg cggccccacc
     6661 atgaacatca aggggaccgc agccgggggg gggaacgctc ttctcattcc catgacggaa
     6721 ttctccatgg gactgacggg ggacatcaat gacatcatga acgcccataa cctggccatg
     6781 gtggccctca ccgcccgcat gcagcacgag agaaactaca atgacgagca actccagcgg
     6841 ctcacccgaa tgcgtcggct ggacgtggat cccacccggg tggaaatggg atgggtcttg
     6901 gatttttgcg cccaatccct tcggaacatc atcatcggca tcggaggcag gcaggacggc
     6961 ttcatgatgc agtccaaatt cggaattgcc gtgagctccg aactgatggc cattctttcc
     7021 attgttcgcg acctgcccga cttgaagaaa cggatgaatg agatcaccgt ggctttcgat
     7081 aagaggggca aggcgatcac caccggggac ctcgaggtgg gcaacgccat gacggcatgg
     7141 atgcgaaaca ccatcaaccc aaccctcatg tgcaccgccg agtaccagcc ctgcatggtt
     7201 cacgcgggac ctttcgccaa tatcgctgtg ggacagtcct ccatcatcgc cgaccgaatc
     7261 ggcctcaaga tgttcgacta ccacgtgacc gagagcggat tcgccgcgga catcggcttc
     7321 gagaaattct ggaacgtcaa gtgtcgcaac agcggcctca ttccccacgt atccgtgctc
     7381 accaccacca tccgggcact gaagatgcac ggcggcggcc ccaaagtggt ggcggggctc
     7441 ccgctaccgg aggcctacac caaggaagac ctaggtttgc tcgagaaggg cgttcccaac
     7501 atggtccatc atatcaacac cattcgcaag gcgggcatca acccggtggt gtgcatcaac
     7561 gccttccata cggacaccaa ggatgaaatc gcgttggttc gcaaatacgc cgaggcagcg
     7621 ggggcgcgct gcgccgtatc cgaacactgg gccaaagggg gcgacggtgc cctggagttt
     7681 gccgacgccg tggtcgacgc ctgcaacgag aagaacgagt tcaagttcct gtatccgctg
     7741 gaaatgaagt tgagagaccg ggtggccatg attgcccggg aagtgtacgg tgccgacggg
     7801 gtatcctgga cacccgaggc cgaagccaag gcaaagttgc tcgagtcgga tgccaagtac
     7861 gacgatttct ccaccatgat ggtcaagact catctgagtc tcacccacga cccgacacgc
     7921 aagggagttc ccaagggctg gaccctcccc attcgggacg tcctgatcta ttcgggcgcc
     7981 aagttcctct gcccttgcgc aggaaccatc agcctgatgc cggggacttc ctcggatccc
     8041 gccttccgaa gggtggatgt ggacgtgaat accggcaagg tcacgggact cttctaaaca
     8101 ccggtccccg agtctctcga caacgttagc gatcaccaaa gaggcaggac gagatcgtcc
     8161 tgccctcttt cagggaggaa aaacatgccg gccaagctgc tcaaaggggc cgaagtggcc
     8221 aaggaaatcc gcgaagagct caaagcggaa gtacggcaac tccagtccaa acattccgtg
     8281 gtcccgggat tggtcaccat cttggtgggg gaaaacccgg cgtctcagag ctatgtccgg
     8341 gccaagcaaa agacggccca cgaactggga ttctactccg tccaggacaa ccaaccggca
     8401 gacctgagcg aagaagctct cctggggctc atcgacaagt acaatcgaga cccaaagatt
     8461 gacggcatac tggtgcagct gccgctgccc aagcacgtca acgagaatcg ggtactcctg
     8521 tccatcgacc ccggcaagga cgtggatgcg ttccatcccg tcaatgtcgg aaagctgatg
     8581 atcggagaac cggactatct tccctgcacc cctgcgggaa tccaggagct gctggtccgt
     8641 tcgggaactg agatttccgg ggcggaagtg gtggtggtgg gtcgatccaa catcgtagga
     8701 aaacccatcg cggtcatgct tatgcaaaag agcagagggg ccaacgccac ggtcacggtt
     8761 tgtcacaccc ggaccaggga cgtcaagtac cacacccgcc gggcagacat cctcattgcc
     8821 gcggccgggg tcacggaata cgtcaaaggg gacatgatca aacccggggc ggtggtcatc
     8881 gacgtcgggg tcaacgaagt gggcaagact ccggacggca agcggatcct caagggagac
     8941 gtggccttcg atgaggcggt ggaaatcgcc agcgccatca ctcccgtacc gggaggcgtc
     9001 ggtcccatga ccatcaccat gttgatgaag aacaccgttc gtgcctgcaa ggtacacaac
     9061 aagatcaaag actgaggtcg gtcatcctgc cgaccttatg gaaaaacccg tggaggtccg
     9121 aagtacggct cctcacgatt gagggtccac aatccccgtc cagccggtgt ccgccccgga
     9181 tcgaatccgg ggcccgccgg attcacgatc ggttatggtt gcatcagtgg atgcccattc
     9241 ctggggggct ttaccggatc tgcgggtttt tcttttgacc cggtggaggg tccgccccgt
     9301 gatccaaagg ccttggaagc ttctgttgat cgatgatgac cccggcatcc gcaaagtcat
     9361 gacgatttcc ctggaagacg ccggatacac cgtttggacg gccccggacg gggaatcggg
     9421 catccgggcc tttgaggaga agtccccgga catcgtcatc acggacatcc ggatgccggg
     9481 aatgaacggc atagaggtcc tgcgcaggat caaggagctg gacccggaca aggaggtcat
     9541 cgtcgccacg gcctttggag atttggacct ggccgtcgag gcgctccaac tggatgcttc
     9601 cgacttcatc accaagccca ttcacgagga cgccctgttt gttgccctca aacgggccca
     9661 ggagcgctac tccacccgca aggagctgcg ggactacacg tccctcatcg aagagcgatg
     9721 gatgcagacg gccgaagagc tggccaagac cttccatttt cagaagatgc tcatcgacag
     9781 ctccatcgac gggatcgtcg cctgcgaccc ggccggcaag gtgatggttt tcaacaagag
     9841 tatggagcag atgctgggct acccggcaaa ggaagcggtg ggaaagatgc tcctggacga
     9901 tttcttctgg tccggggaag acaaaaaatt ccaccataag ctggcctcgg aagaattcgg
     9961 gggcaaagat cgactctacc tctacgagtc caccctccgg gacagaacgg gccacaagat
    10021 ccccgtccag ctctccgctt cggtgctgtt ccacgaaggc cgggtggcgg gcatcgtggg
    10081 attctttcgg gacctgaggg agattcgcag gctcacccag gaatttgccg accaggcccg
    10141 tctcctccac caggacaaga tgatttccct gggaaagctg gctgccagcg tggtgcacga
    10201 gatcaacaat cccttggcgg ggatcctcaa ttatacgcgc ctcatgatca agatgttgtc
    10261 ccgcgggccc ctgtcccccg agtccgccga gaggttcgcg gggtatctgc aactcatgga
    10321 aagcgaactg agccgctgct ccaagatcgt ttccaacctt ctggcctttt cccgcaaatc
    10381 caccctcgag tttcgggagg tggacgtcaa cgaactggtc ctcaaatgcg ttctcctgag
    10441 cgagcaccag ctgaccctgc aaaacatccg agtcgagacc cgcctctccg aggggcttcc
    10501 ttccgttcaa ggagacttca atcagctcca gcaatgcctc ttcaacctca ttctcaactc
    10561 cattgacgcc atgccccaag gggggtcatt gacccttcga acgtaccggg aagacccaaa
    10621 gggtcgggtg gtgatggaag tgcgggacac ggggtgcggc attgccgagg aggatctccc
    10681 ctatatattc gatcctttct tcaccaccaa gaccgaaggc aagggattgg ggctgggact
    10741 ttccacggtg tacgggattg tcgatc
//
LOCUS       DAOOVC010000134        10709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_3973_length_10709_cov_5.571804, whole genome shotgun sequence.
ACCESSION   DAOOVC010000134 DAOOVC010000000
VERSION     DAOOVC010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3973_length_10709_cov_5.571804"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PLM79_14205"
     CDS             <1..557
                     /locus_tag="PLM79_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOV87514.1"
                     /translation="PTQQAVTVALRTQQIVAEESGATDTVDPLAGCYYVEAMTDKIEA
                     EIDEYIKKIDAMGGTLVAIEQGFIQKEIQNSAYRFQKEIESGERAYVGINKYIMDEPP
                     PTDLLKVDMKVGEVEAAKLKKLRAERDQAAWKNALDKLREVSKTNENVMPTVIEAVKA
                     KATVGEICDVWREVFGEYRPKEFV"
     gene            602..1006
                     /locus_tag="PLM79_14210"
     CDS             602..1006
                     /locus_tag="PLM79_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOV87515.1"
                     /translation="MTREKKIKIIVAKPGLDGHDRGAKLLARIFAEAGMEVVYTGLRQ
                     TPEMIVSTAIQEDADVVGLSSLSGVHNYFFPKVVELLKEKGLNDVLVVGGGIIPLEDY
                     DFLKQSGVAAVFGPGTPTTQIVDFIQGNVRQK"
     gene            1165..2142
                     /gene="meaB"
                     /locus_tag="PLM79_14215"
     CDS             1165..2142
                     /gene="meaB"
                     /locus_tag="PLM79_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOV87516.1"
                     /translation="MSNNHVQRVLEGSDLAIARLITWLEDEDERALSIMEEIYARTGR
                     AYVIGITGAPGGGKSTLTDKLTRALRRRGQTVGIVAVDPSSPFTGGALLGDRVRMSEH
                     NTDPGVYIRSMATRGFLGGMARATGDVVKVLDASGKDVIIIETVGVGQDEIDVIGIAD
                     TTCLILVPGLGDAIQSMKAGVMEIADIYVVNKADHPGADQLCVEVSVRVEQDLHIKPT
                     GWSPCVVRTVAVEDQGVDLLVEAIDRHRRYLEESGQYLEKRRERTRQETLRMVHNELF
                     RIVRQRLQDSGRLDRLVQEILDHKRDPYSTMREIVNKWLSFPQNQKEAL"
     gene            2139..2552
                     /gene="mce"
                     /locus_tag="PLM79_14220"
     CDS             2139..2552
                     /gene="mce"
                     /locus_tag="PLM79_14220"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOV87517.1"
                     /translation="MKILKVDHIGIAVTSIEDAKKLYTDTLGLPHLGSETVAEQKVTT
                     AFFPVGDTEVELLESTAPDGPIAKFIEGNKGRGGVQHIAFRVENIEEALAELKAKGVA
                     LIDQTPRMGAGGAKIAFLHPKATFGVLVELSERKD"
     gene            3034..4272
                     /locus_tag="PLM79_14225"
     CDS             3034..4272
                     /locus_tag="PLM79_14225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87518.1"
                     /translation="MEWERLLFRILADASPQPLVVTDPERSRFYYVNPAWELRFGWKT
                     EEVGTLNLPDCYPRGSARILAEIAASVSSPSAANRWEGVLEVLDVQGQSHFLRTRVRA
                     LRKPRGKKILAWVLIHDEHEEFPRDKSPSQKEEPYRQVVESQDELICRFLPDGTITFF
                     NGAFGSHFGLRPGERTAANLFSLVPAEEHQRLRAFLNSPSPDFPSFTREYRIPQPGGG
                     FRWLQWTDRAIFDAKGRISGFQSVGRDITEQRITEEVLREARRELLLAVRERTRQLEE
                     ANTALKVLLNKRDEDEIQLRESILSNIRKLVLPNLERLMLTRLDEKQTAYLHLIRSNL
                     EEVSSPFLRGLSLSFPHLSPMETQVAALIADGRQNKEIASLLGISLNTVLTHRYHLRE
                     KLGLKKAKVNLQSFLKTRRF"
     gene            4679..6682
                     /locus_tag="PLM79_14230"
     CDS             4679..6682
                     /locus_tag="PLM79_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV87519.1"
                     /translation="MKKSSLAARLVMGGVIAVLMPLIVIGVFTIHKATNSLTSVSKEE
                     AAGIAHKLAQTCEVALRNELKVVKTLALERGTIEALPQLGKNQGADAAAYSEKLARYL
                     ADVMKSAGSDYESVLISNAEGLVVADGTNGEYKNISMADRDYFKVSKEGKANIGTVVK
                     SKKTGKPIVPVAAPILGPNGQFLGTMAVLLKIDYLVEEIAGTKTGKTGYAFMTDKTGL
                     IIAHPRKELILELNLTTLKGMERIVSEMMGKKSGVEDYVFEGIKKIAGYAPVELTGWS
                     VALTQPEDEFLEGVYAMRNGTILVAGIFLALTIIAVLFFARRISVPIMRVVEGLNEGA
                     DQVAAASAQVSSSSQSLAEGASEQAASLEETSSSLEEMASMTKQNADHANQANALMME
                     IRRVVDGSNSAMRDLTTSMGEISKSSEETSKIIKTIDEIAFQTNLLALNAAVEAARAG
                     EAGAGFAVVADEVRNLAIRAAEAAKNTASLIEGTVKRIKDGTELVTKTGDAFQLVSTS
                     TGKMGELVGEIAAASNEQAQGIDQINKAVSEMDSVVQQNAASAEESASASEELNAQAE
                     QMKGYVKDLVAVVGGNQNGRQRKGWNRFRSKATTETTVDTKPAPAIHPPKRTGNGKVL
                     IRGDGHAVTAQRPKVSGSRSDPKSAQVTPSQILPLDADDFDQF"
     gene            complement(6810..7616)
                     /locus_tag="PLM79_14235"
     CDS             complement(6810..7616)
                     /locus_tag="PLM79_14235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV87520.1"
                     /translation="MILILAIYLGLGAVAGVLAGLLGIGGGLVIVPMLVFSLTWQGIP
                     QDFIMHLALGTSMATIIFTSISSFWAHHKRGAVQWGVVRRIVPGILLGTFLGTCVASH
                     MTTDYLKGSFVVFVYYVAIQLLTNKKPKPSRELPGQAAMFGVGGIIGVVSSLVGIGGG
                     TLSVPFMVWCNITVHRAVGTSAAIGFPIAVAGTLGYIFNGLQVTGLPGHSFGFLYLPA
                     LVGIVCASVLTAPLGVRMAHSLPVDKLKRLFAVLLLAVGTRMLVGLIRQG"
     gene            complement(7717..8463)
                     /locus_tag="PLM79_14240"
     CDS             complement(7717..8463)
                     /locus_tag="PLM79_14240"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOV87521.1"
                     /translation="MSARKTGPTARKYLYYDIVEEIRGMIRKGQLHVGDKLPAERTLA
                     RTFGVSRNCVRQAIQTLAEKNILESRLGDGTYVSLPDRLIRVDTLALTVRRKDLLKDV
                     IEFRLLLEPQVAYLAAARITPEELDRLKVLVCDQDRRVLAEEPDADLDAAFHGQLVKA
                     CRNRVVREVYEKMGDALNESRAEILRSDSRKRSSVVGHLRIIDALEKKDPKGAFEAMR
                     EHLLAVDRVLFGDDGTIEPVSLSLPEGGWV"
     gene            9193..9870
                     /locus_tag="PLM79_14245"
     CDS             9193..9870
                     /locus_tag="PLM79_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV87522.1"
                     /translation="MKVTNEIFQVGGDRFSSPEDAAVYLLCFAGHAALIDAGCGYAQK
                     RILSNIERCGVPLSAVEAILLTHCHFDHAGGAQSLKKALGCSLVAHELDSPYLENGDD
                     EVTAAAWYGSAMMPVTPDRKLQGPRQTLSLGPRTLEAIHIPGHSPGSTAYLAESEGLR
                     VLFGQDVHGPIHPSLRSNLRDYWNSLELLLTLEADVLCEGHYGVYRGKKAVADFIAGF
                     LTGGRSR"
     gene            complement(9915..10430)
                     /locus_tag="PLM79_14250"
     CDS             complement(9915..10430)
                     /locus_tag="PLM79_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV87523.1"
                     /translation="MMRGKWLGFWSILCLMSFLLPTMTHALEWGTKELDTEKLAVKFY
                     SDVAKGGYKLVTTEELKGWIDQKKPMLIVDTMPFEESYKKNHIPGAVQFLFPIEDLNQ
                     MDDKMKADFEKLLGPDKNRLLVFYCGFTKCTRSHNGALWAKKLGYVNVYRHPGGVKAW
                     MEADYPVEKEK"
     gene            complement(10465..>10709)
                     /locus_tag="PLM79_14255"
     CDS             complement(10465..>10709)
                     /locus_tag="PLM79_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87524.1"
                     /translation="VRGSLPAVSGMLVLFLGVLGFGILNQLDVDCGCFSPDELQAQGR
                     LRAAFLRDVGLLGCTAYLFYWRRRRARLHRPAVSLV"
BASE COUNT         2338 a         3207 c         3184 g         1980 t
ORIGIN      
        1 tgccgaccca acaggccgtg acggtagccc tgcgcaccca gcagatcgtg gccgaggaaa
       61 gcggcgccac cgacaccgtc gatcccctcg ccggctgcta ctatgtggag gccatgaccg
      121 acaagatcga agccgagatc gatgagtaca tcaagaagat cgacgccatg ggcggcaccc
      181 tggtggccat cgagcagggc ttcattcaga aggagatcca gaacagtgcg taccggttcc
      241 agaaggagat cgaatccggg gaacgtgcgt acgtgggcat caacaaatac atcatggatg
      301 aaccacctcc aacggatctt ctgaaagtcg atatgaaggt cggagaagtc gaagcggcaa
      361 aactcaagaa gcttcgggcc gagagagacc aggcggcctg gaagaacgct ctggacaagc
      421 tgcgggaagt ctccaagacc aacgagaacg tcatgccgac ggtcatcgag gccgtgaaag
      481 ccaaagccac ggtcggcgag atctgtgacg tctggcggga agtcttcggt gaatatcgac
      541 ctaaggaatt cgtgtagacg atcggaagat gacataacag tctcaagtgg aggagaaatt
      601 catgacacgc gaaaagaaga tcaagatcat cgtagcaaaa cccggtctgg acggtcatga
      661 tcgcggcgcc aagctgttgg ccagaatatt tgccgaggcg ggaatggaag tggtgtatac
      721 gggattgcgc caaacgcctg aaatgatcgt cagcaccgcc atccaggaag acgccgacgt
      781 ggtcggtctc agcagccttt cgggggtcca caactacttc ttccccaaag tggtggaact
      841 ccttaaggaa aaaggcctca acgacgtgct cgtggtgggc ggaggtatca ttccgctgga
      901 agactacgac ttcctgaaac aatccggcgt cgcagccgtt ttcggacccg ggacccccac
      961 gacccagatc gtggatttca tccaaggcaa cgtccgtcaa aagtaaggac ccaacggggt
     1021 gagagggggt cccgtttccg ggcccccttc tttccacccc gaagaaacgc tctacgcggc
     1081 ccatcatccc cctctactcc atgacgaagg tcccgaagcg acgcggcccc aacaagcatt
     1141 ggcagcgaaa ggaacagcag gttcatgtcc aacaatcacg tacagcgagt cctggaaggt
     1201 tctgatctgg cgatcgctcg tttgatcacc tggctcgagg acgaagacga gcgagcactc
     1261 tcgatcatgg aagagatcta tgcccgaacg gggcgagcct acgtcatcgg gatcaccggt
     1321 gctccggggg gaggaaaatc caccctcacg gacaagctca cccgagccct gcgaaggaga
     1381 ggccagaccg tgggaatcgt ggccgtagat ccttccagtc ccttcaccgg gggtgcgctc
     1441 ctgggagatc gggtccgcat gagcgagcac aacaccgatc cgggggtcta tatccgaagc
     1501 atggccactc gggggtttct gggcgggatg gccagggcca ccggggatgt ggtcaaggta
     1561 ctggatgcct ccggcaagga cgtgatcatt atcgagaccg tgggagtcgg gcaggacgaa
     1621 atcgacgtca tcggtattgc ggacaccacg tgtctcatcc ttgtgcccgg ccttggagat
     1681 gccatccaga gcatgaaggc gggggtgatg gagatcgccg acatttatgt ggtcaacaag
     1741 gccgaccacc cgggggcgga ccagttgtgc gtcgaggttt ccgtgcgtgt cgagcaggac
     1801 cttcacatca aacccacggg atggtccccg tgcgtggtca gaaccgtcgc ggtggaagac
     1861 cagggggtgg atctcttggt ggaggccatc gaccggcacc gccggtacct ggaagaaagc
     1921 ggccaatatc ttgaaaaacg cagggagcgc actcgtcagg agacgctccg aatggttcac
     1981 aacgaactgt ttcgcatcgt tcgacagcgt ctgcaggaca gcgggcgact ggatcggctc
     2041 gtacaagaga tcttggacca caagcgggac ccctacagca ccatgcgcga gatcgtgaac
     2101 aagtggctgt cttttcccca aaaccaaaag gaggcgttat gaagatactg aaggtggacc
     2161 acatcggcat cgcggtaacc agcatcgagg atgcgaagaa gctctatacg gataccctgg
     2221 gcctgccgca tctgggcagc gaaaccgtgg cggaacaaaa ggtgaccact gctttctttc
     2281 cggtgggcga tacggaagtc gaactgctgg aatccacagc tccggacggc cccatcgcca
     2341 agttcatcga gggcaacaag ggccgcggcg gggtacagca catcgctttc cgtgtagaga
     2401 acattgaaga agccctggcg gaactgaaag ccaagggagt cgctctgatc gaccaaaccc
     2461 cgagaatggg agcgggcgga gccaagatcg ccttcctgca tcccaaggca accttcggcg
     2521 tcctagtaga actttcggaa agaaaggact agcacccggc cggtttcctc ggaccggcaa
     2581 gggcacggca ttccgtgccc ttttttgttg ataaggcttt tgctctccca accgctccag
     2641 gcaactcgag gcgagggagt cggctttttc aagactcgcc caccgtgaac gccctctcat
     2701 tcctgcagcc ttcctatgga agaaagcctc tcccacccac acggatattt ctggtgcaat
     2761 cgctcccgtc acggtaaaat tttactgatt ctccgcagag cagctccccc gggaggggga
     2821 gcgtggctgc ggcaacggtg agaggtcagg tcccggaaga gtgggggaag accgctcctc
     2881 acgatgttcg gaaaccccgc tccgggacgg ctttccttgg gagcggctgg acgtcttttt
     2941 ccggtggggc cgacggattc ccgataccgg gtccgctgca tgcgcggggc cctcgtaagg
     3001 acctgccccg tcggggtgat aagggcagct gagatggaat gggaacggct ccttttccga
     3061 atccttgcag acgcttctcc gcaacccctg gtggtcacgg acccggagag aagccgcttc
     3121 tattacgtca acccggcctg ggaattgcga tttggatgga aaacggaaga ggtcggcacc
     3181 ctgaatcttc cggactgtta ccctcgaggg tccgcccgga tcctggccga gattgccgcc
     3241 tccgtctcct ccccgtcggc ggcgaatcgg tgggaaggtg tcctcgaggt cctggatgtt
     3301 caaggtcaaa gccacttcct acggacccgg gtccgggccc ttcgcaaacc ccgtggaaag
     3361 aaaatccttg cctgggtgtt gattcacgac gagcacgagg agtttccccg ggacaagtcc
     3421 ccctcccaaa aggaagaacc ataccggcaa gtggtggaat cccaggatga actcatctgt
     3481 cgtttcctgc ccgacggaac catcaccttt ttcaacgggg cttttgggag ccatttcggc
     3541 ctacgccccg gagaacggac cgcagcaaac ctcttctccc ttgtgcccgc ggaggaacat
     3601 cagaggcttc gcgccttcct gaattccccc tcaccggatt ttccctcctt cacccgggaa
     3661 tacagaatac cccaacccgg tggaggattt cgttggcttc aatggacgga ccgggcgatc
     3721 tttgatgcca agggtcggat ttccgggttt cagtccgtag gcagagacat tacggaacag
     3781 agaatcaccg aagaggtcct ccgcgaggct cgccgggagc ttctattggc ggttcgggaa
     3841 cgaacccggc aactcgaaga ggccaacacg gccctgaagg tgctgctgaa caaacgggac
     3901 gaagacgaaa tccaactgcg tgaaagcatt ctttccaaca tccgcaaact ggtcctcccg
     3961 aacctggaaa ggctcatgct cactcgcctg gacgagaagc agaccgccta cctgcacctc
     4021 atccggtcaa acctcgagga ggtgtcgtcc ccttttctga gaggcctctc cctgagtttt
     4081 ccccacctga gcccaatgga aacccaggtt gccgccctca tcgccgacgg caggcaaaac
     4141 aaggaaattg cttccctgct gggcatatcc ctcaacaccg tcctgactca ccggtatcat
     4201 ctgcgcgaga aattgggcct caagaaggcc aaagtcaacc tgcagtcctt cctcaaaacc
     4261 cgccggtttt agtgactcgt catcactagt ttatccccac tctttcaatt gaagacaacc
     4321 caatcccttg acgaatgaag aagtaagacc gtctcggacc ggaaccgccg ggaggcgttc
     4381 cgaagggggg ttgtcgaaaa gaggaagccg acccgggaga tcgcatgggt gtttgcgtcg
     4441 agtgatcgga aagagagctc ttgctcacgt ctcagaggca tccaaccggc tcccttccct
     4501 tgcgtcgccg gggttgggcg ggagagtgga accgggggct ttgagatcgt tggaccaaag
     4561 ggcggagcac atggcgggaa atactgtggt tgatctttct taaccgttct ctcccggcgc
     4621 aaaaagaagc ggggagggaa gagagccgac cgattcggca gactggaacg gggggaacat
     4681 gaagaagtca tcgttagcgg cacgtttggt catgggtgga gtgattgcgg tcttgatgcc
     4741 cctgatcgtc attggtgtct ttaccattca caaggccacc aattctctca catcggtgtc
     4801 caaggaggag gcggcgggca ttgcccacaa gttggcgcag acgtgcgagg tggcgctccg
     4861 gaacgaactg aaagtcgtca agacactggc cctggaacga ggcaccatcg aggcccttcc
     4921 gcaactgggg aaaaaccagg gggccgatgc ggccgcctat tcggaaaagc tggcccgcta
     4981 ccttgccgat gtgatgaaat cggcgggaag cgattacgag agcgtcctca tcagcaatgc
     5041 agaagggctc gtggtggccg acggcaccaa cggggagtac aagaacatca gcatggcgga
     5101 ccgggactat ttcaaggtct ctaaagaagg gaaggccaac atcggtaccg tggtgaaatc
     5161 caagaaaacg ggaaagccca tagtcccggt ggcggccccg attttggggc caaacgggca
     5221 gttcctcgga accatggccg ttttgctcaa gatcgattac ctggtcgagg aaattgcggg
     5281 gacgaagacc gggaagaccg gctacgcctt catgacggac aagaccggtc tgatcatcgc
     5341 acaccccaga aaagagctca tcctggaact caatctcacc accctcaagg gaatggaaag
     5401 aatcgtgtcg gagatgatgg gaaagaagtc cggggtggaa gactatgttt tcgaggggat
     5461 taagaagatc gccggatatg ctcccgtgga gcttaccggc tggagcgtcg ccctcactca
     5521 gcccgaggac gaattcctcg agggcgtata cgccatgcga aacggcacca tcctggtggc
     5581 cggcatcttt ctggccctga ccatcattgc cgttctcttt ttcgccagga ggatttccgt
     5641 gcccatcatg agggtggtgg agggtctcaa tgaaggggcg gaccaggtgg cggccgcctc
     5701 cgcccaggtt tcctcgagca gccaatccct ggccgaggga gcctcggaac aggcggcctc
     5761 cctggaggag acctcctcct ccctcgagga aatggcctcc atgaccaagc agaacgccga
     5821 tcatgccaac caggcaaacg ctctcatgat ggagatccgc agggtggtgg acggctccaa
     5881 ctcggccatg cgcgacctca ccacctccat gggcgagatt tccaagtcca gcgaggagac
     5941 ttccaagatc atcaagacca ttgacgaaat cgccttccag accaacctgc tggctctcaa
     6001 cgccgccgtg gaggcggccc gggccgggga ggccggggcg ggctttgcgg tcgtggcgga
     6061 tgaagtgcgt aacctggcca tccgggccgc cgaagccgcc aagaataccg cctccctcat
     6121 cgaagggacc gtgaaacgga tcaaggacgg gacggaactc gtcaccaaga cgggggacgc
     6181 cttccagttg gtgtccacca gcacgggaaa aatgggagaa ctggtcggag agattgccgc
     6241 cgcctccaac gaacaggccc agggaatcga ccagatcaac aaggccgtct cggagatgga
     6301 ttcggtggtc cagcaaaatg ccgccagcgc cgaggaatcc gcctcggctt ccgaggagtt
     6361 gaacgcccag gcggaacaga tgaaaggtta cgtaaaggat ctggtggcgg tggtgggggg
     6421 aaatcagaac ggccggcagc ggaaagggtg gaatcggttt cgcagcaagg ccacaaccga
     6481 gacgacggtg gacacgaaac cggccccggc cattcacccc cccaagagaa ccgggaacgg
     6541 aaaggtcctc attcgcgggg acgggcacgc cgtgacggcc cagcggccga aggtctcagg
     6601 gagccgttca gatcccaagt cggcccaggt gactcccagt cagatcctgc cgttggatgc
     6661 ggatgatttc gaccaattct aacccccgcg ccccgggtcc ctgacgggcc gcgggacccc
     6721 tcgccgccgt ttccataccc tgccctcggg atggcggcga ggggttctcc acgagaaggt
     6781 ccccgaggtt cctgcaaacc ccaaccccat cagccctgcc ggatcaaccc gaccagcatt
     6841 cgagttccca ccgccagcag cagaacggca aagagccgct tcagcttatc cacgggaaga
     6901 ctgtgggcca tccggacacc cagtggcgca gtcaggacgc tggcgcacac gattcccacc
     6961 agggcgggca aatacaggaa accaaacgaa tggccgggca ggcccgtcac ctgcagcccg
     7021 ttgaatatgt agcccagggt ccccgcaacc gcgatgggga agccgatggc cgccgacgtc
     7081 cccacagccc ggtgcaccgt gatgttacac caaaccatga aggggaccga caaggtccct
     7141 cccccgattc ccaccaggct ggacaccacc ccgatgatgc ctccaacccc gaacatggcc
     7201 gcctgtcccg ggagttcccg ggacggcttg ggcttcttgt tggtgagcag ctggattgcc
     7261 acgtaataga cgaaaaccac gaaggagccc ttgagatagt cggtggtcat gtgggaggca
     7321 acacaggttc ccaggaaggt ccccagcagg atccccggaa cgatgcgccg cacgacgccc
     7381 cactggaccg cgccccgttt gtgatgagcc cagaagctcg atatcgaagt gaagatgatg
     7441 gtggccatgg aagtccccaa ggccaggtgc atgatgaaat cctgaggaat cccctgccag
     7501 gtgagactga acaccaacat gggcacgatc accagtcccc cgccgattcc caacagtccc
     7561 gccaacactc ccgccaccgc tcccagtccc aaatagatcg ccagaatgag aatcatgtcc
     7621 aactcccctt gttcctccaa ggaatccctt ccaacgcctc caagacccac ggtctgccgg
     7681 aacgggaaag atccgtaccg tccgtctttg cggttttcac acccaacctc cctcgggtag
     7741 agagaggctc acaggctcga tggtcccgtc gtcgccgaac aggacgcggt cgacggccaa
     7801 aaggtgttct ctcatggctt cgaaggcacc cttggggtcc ttcttttcca gggcgtcgat
     7861 gatcctgagg tgccccacca cggaactccg cttcctggag tcgctgcgaa ggatctcggc
     7921 gcggctttcg ttcaatgcat cccccatctt ttcatagacc tcccggacca cacgattcct
     7981 gcatgccttt accagttgcc cgtgaaacgc cgcatccagg tctgcatccg gctcctcggc
     8041 caggacccgc cggtcctggt cgcagacgag gactttcagc cgatccagtt cttccggggt
     8101 gatgcgcgcg gcggccaggt acgctacctg gggttccagg agcagccgga actcgatgac
     8161 gtctttcaga aggtcctttc gacggaccgt cagggccagg gtgtccaccc ggatcaagcg
     8221 atccgggaga gatacgtagg tcccgtctcc caggcggctc tcgaggatgt ttttttccgc
     8281 cagggtctgg atggcttgtc gaacgcagtt tcgggagacc ccgaaggtcc gggccagggt
     8341 cctttccgcg ggcagtttgt cccctacgtg gagctgtccc tttcggatca tgccgcggat
     8401 ttcctcgaca atatcgtagt agagatactt gcgggcagtg ggaccggttt ttcttgccga
     8461 catcgacctt gcttcctttt cgtgcctact cgtgctcccg ccctggacaa acggtggtcc
     8521 atccaaaaag gttaataacg cattttggta ctaccaataa cccattccag gcgctgggac
     8581 aaccgatttt gctagggccg ccgcattttt cggaaaacgg gcgcaagccc cccgactccg
     8641 gcccgtcgac cgggaccggt gctccgtggg caggtcggtg gaaccattct ttggaaggag
     8701 ccccgggcag gacccgaggg tcaacgagaa tcgggcctgg gatcaggcag ggtttgatga
     8761 tagactgttt gaaagctcga ggacgcttgg ggctttcccc ctgccctgcc ggcccgaaaa
     8821 ccccggccgt acattgcaaa gccgtcggtc gagggggaga agcctgagtc ggcgtcgtgg
     8881 gatgctgtaa gggctcttgt cgacgtgcgt tcttcacggg attggcgggg cggggagcct
     8941 cgcttcacgg aatccggcgg ccgggtagac ccaagaggag gtttcttgag atcggcagaa
     9001 ggaagcggcg ggtctgtgcg cggccgcagg catcggacgg atcgccgtgc aggcccaagg
     9061 ggaggcccca tcgagaccga ccgcggattc ggattgcgga cgtccggccg tcccccgggg
     9121 taccgactac gaagcacctc aaattttcgc acagggcctt gccccgattc agagaggttt
     9181 gcaggggtcc atatgaaagt tacgaacgaa atctttcagg ttggcggaga ccggttcagt
     9241 tcccccgagg acgctgcggt ttatctcctt tgcttcgccg ggcatgccgc cctcatcgat
     9301 gcgggatgtg ggtacgccca gaagaggatc ctctccaata tcgaacggtg cggcgttccc
     9361 ctatccgccg tggaagccat tctcctcact cactgtcact tcgaccatgc ggggggcgcc
     9421 caatccttga agaaagccct cggctgcagc ctggtggccc acgagctcga ctctccgtac
     9481 ctggagaatg gggacgatga ggtgaccgcc gccgcctggt acggctcggc catgatgccc
     9541 gtgactccgg acaggaagct ccagggtcct cgtcagaccc tgtctctggg accgaggacc
     9601 ctggaggcga ttcacattcc cggccattct ccgggatcca cggcctacct ggcggaatcc
     9661 gagggtttgc gggtactctt cgggcaggac gttcatggtc ccattcaccc cagcctccgg
     9721 tccaatctcc gggactattg gaactccctg gaactgctgc ttaccctgga ggcggacgtc
     9781 ctctgcgaag gccactacgg ggtctatcgg ggaaagaaag cggtggcgga ttttattgcg
     9841 gggtttttga ccggcggccg ttcccgctga agcctcctta cgggaccggc cgacggcccc
     9901 attcttccgg attcctactt ttctttctca acagggtaat cggcttccat ccaggctttg
     9961 actcccccgg gatgacggta gacgttgaca tatcccaact tctttgccca cagagcgccg
    10021 ttatggctgc gggtgcactt ggtgaatccg cagtagaaga ccagtagccg gttcttgtcg
    10081 gggcccagga gcttctcgaa gtccgccttc atcttgtcgt ccatttgatt gaggtcttcg
    10141 atggggaaca agaactgcac ggcaccgggt atgtggttct ttttatagct ttcctcgaag
    10201 ggcatcgtgt ccacgatgag catgggcttt ttctggtcaa tccatccttt gagctcttcc
    10261 gtggtgacca gcttgtaccc gcctttggct acgtccgaat agaacttgac cgcaagcttc
    10321 tccgtatcca attccttcgt tccccactcc agggcatggg tcatcgtggg aaggagaaag
    10381 ctcatgagac aaaggatcga ccagaacccc aaccattttc cgcgcatcat cctcacaact
    10441 cctttccatc gtcacggtcg aaaatcacac caaagaaact gccggtctat gcaggcgggc
    10501 ccgtcgccgg cgccaataga aaaggtaggc cgtgcacccc aggagcccca cgtccctaag
    10561 gaaggcggct ctcaggcgcc cctgggcctg caactcgtcc ggggaaaagc atccgcaatc
    10621 cacgtccagc tggtttagga ttccgaatcc caagaccccc aggaacaaga caagcattcc
    10681 ggaaacggcg ggaaggctcc ctcttacat
//
LOCUS       DAOOVC010000135        10518 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4066_length_10518_cov_5.682500, whole genome shotgun sequence.
ACCESSION   DAOOVC010000135 DAOOVC010000000
VERSION     DAOOVC010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10518
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_10518_cov_5.682500"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLM79_14260"
     CDS             complement(<1..923)
                     /locus_tag="PLM79_14260"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOV87525.1"
                     /translation="MASDLHCFLLGTGGMMPMPERMLTSLAVRLGGRTYLFDAGEGTQ
                     VSWKRLRLGLRGFEVLALSHLHADHCLGVPGMMMLRAQMDFPGPLTVLGPPGTRSFLR
                     DLQSNLDYHLNYPVLVREWETEAGETAYEDPWVRILWRPLEHTRFCLGYRIEEPQRPG
                     RFDPLAARALGVPEGPLWGMLQRGRPVLNEAGESVYPEQVLGPPRRGRSVAYVTDTLP
                     GPNALTLCRDADLAFVEGMFLAEDADHARAKEHMTVDQGAQLGLEAGARLTVLIHLSP
                     RYNGRDRDRLESAVRPHGNRVRVGRDLESYPV"
     gene            1283..1356
                     /locus_tag="PLM79_14265"
     tRNA            1283..1356
                     /locus_tag="PLM79_14265"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1315..1317,aa:Gln,seq:ctg)
     gene            1362..1437
                     /locus_tag="PLM79_14270"
     tRNA            1362..1437
                     /locus_tag="PLM79_14270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1396..1398,aa:Glu,seq:ctc)
     gene            complement(1624..2586)
                     /locus_tag="PLM79_14275"
     CDS             complement(1624..2586)
                     /locus_tag="PLM79_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87526.1"
                     /translation="MPKEITLQELDTGRFIEEKVRDIREAVGDGIAINALSGGVDSST
                     VTLLGHRALGSRLRTVFIENGLMREGEARQVTGWFRELGVAVEVVDARNEFFAALAGV
                     VDPEEKREAVTQTFYRDVFGRIVKESGARHLLQGTILTDIDETVAGIKRQHNVFEQLG
                     IDPQEAFGYRILEPLIELRKDGVRAVGKALGLPSEVFTRIPFPGPALAARVIGAVTPE
                     RIETVRKATAVVEGLLGDTGAFQYLAILHEDRVTGMRNGKRDFGLQIEVRCWDSVDAR
                     VAQPTEISFDVLKRLAREITREVPGVVSVTYNITSKPPSTIEAV"
     gene            3225..4418
                     /locus_tag="PLM79_14280"
     CDS             3225..4418
                     /locus_tag="PLM79_14280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV87527.1"
                     /translation="MGRSAKDPVPTYEALLVLCCVVTSMCYLGSHMRIPVVPLFARTL
                     GADTFQVGAVNASFLFTAALLSLPLGLLSDRLGRKRLITAGLLIISLSSFLLCFSTTV
                     LEMIGIYLLFGTGLAAFAPTMMSFVADFSPPTHLGRSYGWYTLALYGGMSTGPALGGA
                     LAGWVGFQWTFAVSGAVTLAVLFLVAGLLPRAGQVLVNRPSKRPTGEVAKELVKNAPL
                     LACWVATLGGCLGLGMFVTFVPLYAQDQGVRVEQIGFIFGLQALCNALSRIPFGRLSD
                     RAAKRSRLATAGLLGLSVSIAGLGPARGMGAFLLFAAGLGISMGIAFTAIGALISEVV
                     APDARGLAMGGYNSAIYIGMMVSSLVMGAVIREIGFKNAFLIVAVLNLGATGLFHRVF
                     SHSPA"
     gene            complement(4524..5051)
                     /locus_tag="PLM79_14285"
     CDS             complement(4524..5051)
                     /locus_tag="PLM79_14285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOV87528.1"
                     /translation="MTKQHERTASSHTGPHSFQMKSWISCAPFERLLNMEIVEASEGH
                     ATLTMPFLVDYAQGAGLMHGGALVSLADTAVVMAIKSILPPLSHFATIHCETRFLRPV
                     RKGIVTARAKVISRQDREFQGQATLYDEDGSTVLVFSSTFKMAGNAKIRGVVFEGDSA
                     VPNHEPIGPCRKPKD"
     gene            complement(5209..6423)
                     /locus_tag="PLM79_14290"
     CDS             complement(5209..6423)
                     /locus_tag="PLM79_14290"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV87529.1"
                     /translation="MSSDPGIPGRCTLFPDSRLCWKDQTGFVPAKAFDPGNWDAPPRT
                     PAHRTACGGQGRRLGFPLPRGEGWEEEGSAPKAPPPPPYLPKRGRGPFVRPRVNHAGE
                     EKPGKEHGIMKEKIGFMGLGIMGRAMAANIARAGYPLTVYNRTPGKWEALEELEVETA
                     PSPEALAEKSDVVIAMVTGPEALEQLLWGEKGAARAFEPGKVFINMSTVSPSYTKDLD
                     RRLASTGVIFLDAPVSGSKKPAGDGTLLILAGGPEDSVEALTPLLKTMGSQVIYCGRV
                     GQGSMMKMANNLLLTLMIEGLAEALNFGRKGGLSDEAVLEVVLNGPLGCPIFQMKGRA
                     FAERSFDPNFPLKHAAKDLKFLVDTAYELGAPVPVGHTLLHLYRAGVARKWGDLDIGA
                     ILKVLEHLNPAE"
     gene            6853..7140
                     /gene="groES"
                     /locus_tag="PLM79_14295"
     CDS             6853..7140
                     /gene="groES"
                     /locus_tag="PLM79_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOV87530.1"
                     /translation="MKLRPLHDRVVVKRVDEEQKTAGGIIIPDTAKEKPQQGRVLAVG
                     RGRILEDGKVVPLMVKAGDLVLFSKYAGTDIKIEGEEHLIMREDDILAILE"
     gene            7180..8811
                     /gene="groL"
                     /locus_tag="PLM79_14300"
     CDS             7180..8811
                     /gene="groL"
                     /locus_tag="PLM79_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699195.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOV87531.1"
                     /translation="MPAKEIKYYAEARERIMKGVNALADAVKVTLGPKGRNVVLEKSW
                     GSPNITKDGVTVAKEIELADRFENMGAQMVKEVASKTSDVAGDGTTTATILAQAIYRE
                     GSKLVAAGHNPMALKRGIEAAVAAAVEELKRMSKPTKDQKEIAQIGTISANNDATIGN
                     IIAEAMNKVGKEGVITVEEAKSMETTLDVVEGMQFDRGYISPYFVTDPERMEVALEDA
                     LILIYEKKLSNMKDLLPVLEQIAKTGSPLLIIAEDVEGEALATLVVNKLRGTLKVCAV
                     KAPGFGDRRKAMLQDIAILTGGQMISEDLGIKLENIGMHDLGRAKTIRVDKDNTTIVD
                     GAGSKEAIEGRVKQIRTQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATETEMKEK
                     KARVEDALNATRAAVEEGIVPGGGVALLRCLNALDNVKVSGEESFGVSIVKRALEEPV
                     RQIANNAGHEGSVVVEKVRNSSGSFGFNADTEVYEDLTEAGVIDPTKVVRFALQNAAS
                     VSSLLLTTEAMVAEKPKKKEAAPPMPGGPGMEDMY"
BASE COUNT         2022 a         3173 c         3311 g         2012 t
ORIGIN      
        1 acgggatagc tctccaggtc ccttcccacg cgaacccggt ttccatgggg tcggaccgcg
       61 gattccaggc ggtccctgtc ccggccgtta tagcgtggac tgaggtggat gagtaccgta
      121 aggcgggctc ccgcctcgag gcccagttgg gctccttgat ccacggtcat gtgttccttg
      181 gcccgggcat ggtccgcgtc ctcggcaaga aacattccct cgacgaaggc aagatccgcg
      241 tccctgcaga gggtgagggc attcgggccg ggaagggtgt cggtcacgta ggccacggat
      301 cttccccgcc tcgggggacc caggacctgt tccgggtaga ccgactctcc agcctcgttc
      361 aaaacgggcc gtccccgttg gagcatcccc cacaacggcc cttcgggaac gcccagggcg
      421 cgagcggcaa gaggatcgaa tcttcccggc cgttggggtt cctcgatccg gtagcccagg
      481 cagaaccggg tgtgctcgag ggggcgccac aggatgcgca cccatggatc ctcgtaggcc
      541 gtctcccctg cctccgtctc ccattcccgg accaggacgg gatagttgag gtggtagtcg
      601 aggttcgatt gaaggtcccg gagaaacgaa cgggtcccgg gaggtcccag aacggtgagg
      661 ggccccggga aatccatttg ggcacgcagc atcatcatgc cgggcacccc caggcaatgg
      721 tcggcgtgaa ggtggctgag ggccaggacc tcgaaccccc gcaggcccag ccgcagccgt
      781 ttccaggaaa cctgggtgcc ttctcccgca tcgaaaaggt aggttcttcc ccccaaacgc
      841 acggccaggg aggtgagcat gcgctcgggc atgggcatca tgccgccggt tcccaggaga
      901 aagcagtgaa ggtccgaagc catggggttc gatccctgaa ggtggacgaa tgggccggag
      961 gcggccccaa gagatcatag gcgaatcccg ggaaggatgc aaaggagaag ggcacccggg
     1021 cggagggaga cgaaagggga ggggggaagg ggaagaaggt tgggggcaat cgccggaggt
     1081 cggagggcag cgggtagacg ggaaaggatt cgaatcaggg tgaagggacc cgggagggac
     1141 gtaaaaggga gtgggcgtga aggggggagg aacgagcggg aaaccaaggg cccggcggtg
     1201 agatatcccg gccgacgggc cggggactca tcttccttct tgacttctcc cgggaacccc
     1261 attatgtatc tagcgatttt tctgggggat cgtccagcgg caggactgca gactctggat
     1321 ctgcctacct aggttcgaat cctagtcccc cagccatacg aggtcccatc gtctagtggc
     1381 ctaggatatc ggcctctcac gccgaagaca cgggttcgaa ccccgttggg actaccagcc
     1441 aagagagtcg gcaacctgat cttcgatttc gaggggggtt gccgagcctg tttttgggcc
     1501 cccgcgggac cgcacccggg ttcgagggtc ccgggactgc ttcttcgccc tccccgcggt
     1561 cccgatttcg ggacccctcg aaatgacaat gaaggaaaac cgctcaggtc ggctttctat
     1621 aactcaaacg gcctcgatgg tggaaggagg cttggatgtg atgttgtagg ttacgctgac
     1681 cacccccggc acttcccggg taatctcccg ggccagcctt ttgaggacgt caaaggaaat
     1741 ctccgtgggc tgagccaccc gggcatccac actgtcccag cagcggactt cgatctggag
     1801 gccgaagtcc cgcttgccgt tgcgcattcc cgtcacccgg tcttcgtgta gaatggccag
     1861 gtattgaaac gctcccgtgt ctcccaggag accctcgaca accgcggtgg ctttgcgaac
     1921 cgtttcgatc cgctcggggg tcaccgcccc gatcacccgc gcggcaaggg ccggtccggg
     1981 aaacggaatg cgcgtgaata cctccgaggg gagccccagg gcttttccca ccgcccgaac
     2041 gccgtccttg cgaagctcga tgaggggttc gagaatccgg taaccgaacg cttcctgggg
     2101 atcgatgccc agctgttcaa agacgttgtg ctggcgcttg atcccggcaa cggtttcgtc
     2161 aatgtcggtg agaatcgttc cctggaggag gtgccgggcg ccgctttctt tcacgatgcg
     2221 cccgaatacg tcccggtaga acgtctgggt aacggcttcc cgcttttctt ccgggtcgac
     2281 gaccccggcc agggcggcga agaattcgtt gcgggcatct acgacctcga cggcgactcc
     2341 cagctcgcgg aaccaccccg ttacctgccg ggcttcccct tcccgcatga ggccgttttc
     2401 gatgaaaacg gtcctgaggc gacttcccag ggctcgatgt cccaggaggg tgaccgttga
     2461 ggaatcgact ccgccggata gggcgttgat ggcgatcccg tcccccacgg cctctcgaat
     2521 gtcccgtact ttttcctcga taaaacgccc ggtatccagc tcttgaaggg tgatttcctt
     2581 tggcatacga cggcaatcct ttccctggga agtgttcgtg cgattcaaga cggtccggga
     2641 agccgggtcg aggaaagccc atcccgccca gctccggcct tgtgcttccg cctgcttccg
     2701 ccccgggaca ccggggtccc ggacgccgag gctccctgca acgtggcccc gtccggtccc
     2761 cccatactag agacaacccc cggggggatg caagggtcaa aaaggttgcc cgcctctccc
     2821 ttgcttcgaa agggtcccgc cgcctttctg cgtgtccgaa agggggccga ttcgtgcccg
     2881 ccggcccatt cctcttctca ccgacgcaaa tcagtggcgg gggaagcagg gtctggtata
     2941 aaagggatgt agggcacggc cttccagaaa agaacatccg gtcccgggtt cggtgattta
     3001 tgcggcaacc tttccccgtg cggaggatgt acgggatttc ggcccgcagc gggtacggat
     3061 gtcccggggc gggacctcat cccgggtccc tggttttccg gatcgaggga cgaggatgtc
     3121 cggggttttg ggattcttcc aaccggaaaa gggcaggggg cgcaggaaag aggcactccc
     3181 ttcccggaat cccgacggac gcaagcgcaa caaggaatga ctccatgggt aggagcgcca
     3241 aggaccctgt gccgacctac gaagcgctgc ttgtgctttg ttgtgtcgtc acttccatgt
     3301 gctacctggg atcgcacatg cgcattcccg tggtgccgct cttcgcccgc accctggggg
     3361 ccgacacctt ccaggtgggg gccgtcaacg cgtccttcct cttcacggcc gcgctccttt
     3421 ccctgcccct gggccttctt tcggaccggc tggggagaaa gcgtctcatc acggcgggcc
     3481 tgctcatcat ttcgctgagc tctttcctgc tttgcttcag caccacggta ttggaaatga
     3541 tcggaatcta cctgctcttc ggcaccggac tcgcggcttt tgccccgacc atgatgtcct
     3601 ttgtggccga tttttctccg ccgacccacc tgggccgttc ctacgggtgg tacaccctgg
     3661 ccctgtacgg gggcatgagc accggcccgg ccctgggcgg ggcgctggcg gggtgggtcg
     3721 gttttcagtg gacctttgcc gtgtccgggg ccgtcaccct tgccgtcctc ttcctggtgg
     3781 cgggcctgct gccccgggcc gggcaggtcc tcgtgaaccg tccttccaaa aggcccaccg
     3841 gagaggttgc caaagaactc gtaaagaacg ccccccttct ggcctgctgg gttgccacct
     3901 tggggggttg cctgggtctg gggatgtttg tgaccttcgt gccgttgtac gcccaggatc
     3961 agggggtaag ggtcgagcag atcggcttca ttttcggtct gcaggccctc tgtaacgccc
     4021 tctcccgcat tcccttcgga cggttgagcg accgggcggc aaaacggagt cggctggcga
     4081 cggcgggcct cctgggtttg tccgtctcca tcgcggggct cgggccggcc cgggggatgg
     4141 gtgccttcct gttgtttgct gcggggttgg gtatcagcat ggggattgcc ttcacggcaa
     4201 tcggggcgct catttcggag gtggtcgccc ccgatgctcg ggggcttgcc atgggcggct
     4261 acaacagcgc catctacatc gggatgatgg tcagttccct ggtaatggga gcggtgatcc
     4321 gcgagatcgg attcaagaac gcttttctca tcgtggccgt gctgaacctc ggggcgacgg
     4381 ggctgtttca ccgggtgttc agccattccc ctgcgtagcc cgcgattcga ggcgtgcagc
     4441 acccagggag cgtgtgcgag tccacaacgc ggcgaaaacc cgagggcacc cgtacaggag
     4501 tggtttgcag gctcaaaccc gattcaatcc tttggtttcc ggcaaggtcc gatcggttcg
     4561 tgattcggga cggcagaatc gccctcaaaa acaactccgc ggatcttggc gtttccggcc
     4621 atcttgaagg tcgaggaaaa gaccaatacc gtgctgccgt cctcatcata gagagtagcc
     4681 tgcccctgga actctcggtc ctgccggctt atcaccttcg ctcttgccgt gacgatcccc
     4741 ttcctgaccg gtcgaaggaa cctcgtttcg caatgaatgg tggcgaaatg ggacaggggc
     4801 gggaggatgc tctttatggc catgaccacc gccgtatccg ccaggcttac cagggcccct
     4861 ccgtgcatga ggcccgcacc ctgggcatag tccacaagaa acggcatggt cagcgttgcg
     4921 tgcccctcgg acgcctccac gatctccatg ttcaggaggc gctcgaaagg ggcgcaactg
     4981 atccagcttt tcatctgaaa tgaatgcggg cctgtgtgtg aggatgccgt ccgttcgtgt
     5041 tgcttcgtca tgtgcgatgg gtctcccttc cggaacgtga cttccaatga acccggcgcg
     5101 gacgggccga gcggtagcag gacgccacgg cttcgtggtc ccgaccaatg aacccggcgc
     5161 ggaggcgccg agccctgcga agccgggttc ccggctgttc cgggccgctc attccgcggg
     5221 gttcaggtgt tcgaggacct tcaggatcgc tccgatgtcc aggtctcccc atttcctggc
     5281 cactcccgcg cggtacaggt gaagcagggt gtgacccacc ggaacggggg ctcccagttc
     5341 ataggccgtg tccaccagga acttgaggtc cttggcggcg tgcttcaggg gaaaattcgg
     5401 gtcaaaggac ctttccgcga aggcccggcc cttcatttga aagatgggac agcccagggg
     5461 tccgttcaga acgacctcga gcacggcctc gtcggagagc ccgcccttgc gaccgaaatt
     5521 gagggcctcc gccaggccct cgatcatcag ggtcaagagc aggttgttgg ccatcttcat
     5581 catcgagccc tggccgaccc tgccgcaata gatcacctgg gaccccatgg ttttcagcaa
     5641 gggagtgagg gcctcgacgc tgtcctcggg accgcccgca agaatcagca gggtcccgtc
     5701 cccggcgggc tttttcgatc ccgaaacggg ggcgtcgagg aagatcaccc cggttgaagc
     5761 cagccgacgg tccaagtcct tggtatatga gggagaaacg gtgctcatgt tgatgaaaac
     5821 cttgccgggc tcgaaggccc gggcggctcc cttctcaccc cataggagct gttccagcgc
     5881 ctcgggaccg gtcaccatgg cgatgaccac atcggatttt tcagccaggg cttccgggct
     5941 gggagcggtc tccacctcca gttcttccag ggcctcccac tttccgggag tacggttgta
     6001 caccgtcagc gggtaacccg cccgggcgat attggccgcc atggcacggc ccatgatccc
     6061 cagtcccatg aacccgatct tttctttcat gatcccatgt tcctttcccg gcttctcctc
     6121 gccggcgtgg ttgactcgag gacggacgaa gggtcccctc ccccgcttgg ggagataggg
     6181 tgggggcggg ggggctttcg gtgccgatcc ttcctcctcc caaccctctc ctcgagggag
     6241 agggaaccca agtcgacggc cttgcccccc gcaggcggtc cggtgcgccg gtgtccgggg
     6301 gggggcgtcc caatttcccg gatcgaacgc tttggctggt acaaatcccg tctggtcctt
     6361 ccagcacaag cgggaatccg ggaagagcgt acatcgtccg gggatgcccg ggtccgagga
     6421 catcccgagc cgatgctttc agaaccgctc cacttgagat tttccggagc caagtccagg
     6481 agggatgggt gtcttgcccg cctcctccga ccaggtttcc atactgcttc ccagggtcga
     6541 gtgcaagtcc cttggcattt cctggggggg aaagaacggt tcccgtccaa agattggggc
     6601 cgatagacat ggttttcgaa gcttgaagga gaattgcggt tgcggatgga gatcgctgcc
     6661 cgcttcgcgt catggatctc gggggagggg atttttgggg cttgatttga gagggaagag
     6721 tttttatttt aggggatgtt agcactcatg gtaagtgagt gccggccgct tcggtgggct
     6781 tccgaggtct acccatagtg gaagttcggt tggatcccaa ttctgtcatc catgaaggag
     6841 gaacattggc gtatgaagct cagacccttg catgatcgag tcgtagtcaa acgtgtggac
     6901 gaagaacaga aaaccgccgg aggaatcatc atccccgaca ccgccaagga aaaaccccag
     6961 cagggcaggg tcctcgccgt cggccgcggg cgcatcctgg aagacggcaa ggtggtcccc
     7021 ttgatggtca aggcaggcga cctggttctg ttctccaagt acgccgggac cgacatcaag
     7081 attgaagggg aagaacacct catcatgcgt gaggacgaca tcctggccat tttggaataa
     7141 ctcgattttc cattgaaacc cacagggagg aacaacccaa tgcctgccaa ggaaatcaag
     7201 tattacgcgg aagcaagaga acgaatcatg aaaggggtca acgccctggc cgacgccgtg
     7261 aaagtcaccc tgggacccaa gggccgcaac gtggtcctcg agaaatcctg ggggtccccc
     7321 aacatcacca aggacggcgt caccgttgcc aaggaaatcg aactggccga ccggttcgaa
     7381 aatatgggtg cccaaatggt taaggaggtg gccagcaaga cgtccgacgt cgccggagac
     7441 ggaaccacca ccgccaccat tcttgcccag gccatctatc gggaagggtc caagctggtg
     7501 gccgcaggcc acaatcccat ggccctcaag agagggatcg aagccgccgt ggccgccgca
     7561 gtggaagagc tcaaacgaat gagcaaaccc accaaggatc agaaggaaat cgcccagatc
     7621 ggcaccattt ccgccaacaa cgacgccacc atcggcaaca tcatcgccga agccatgaac
     7681 aaggtgggca aagaaggggt catcacggtc gaggaagcca agagcatgga gaccaccttg
     7741 gacgtggtcg agggcatgca gttcgaccga ggatacatct ccccctactt tgtgacggat
     7801 cccgaacgta tggaagtcgc cctcgaagat gccctcatcc tcatctacga aaagaagctg
     7861 agcaacatga aggacctcct gccggtcctc gagcagatcg ccaagacggg cagccccctt
     7921 ctcatcatcg ccgaagacgt ggagggcgaa gccctggcca ccctggtggt gaacaaactc
     7981 cgaggcacgc tcaaggtctg cgccgtgaag gcccccggtt tcggtgaccg cagaaaggcc
     8041 atgctccaag atatcgccat cctcaccggc ggccagatga tttccgaaga cctggggatc
     8101 aagctcgaaa acatcggcat gcacgacctg ggtcgagcca agaccatccg agtggacaag
     8161 gacaacacca ccatcgtgga cggagccggc tccaaggaag ccatcgaagg ccgggtgaag
     8221 cagattcgta ctcaaattga agaaaccact tcggactatg accgtgagaa gctccaggag
     8281 cgcctggcca aactggtggg cggcgtggcg gtcatcaagg tgggggccgc aaccgagacg
     8341 gaaatgaagg aaaagaaggc ccgggtggaa gacgccctga atgccacgcg agccgccgtc
     8401 gaggaaggga tcgtgccggg cgggggcgtg gccctgctgc gttgtttgaa cgccctggac
     8461 aacgtgaagg tctccgggga ggaatccttc ggcgtttcca tcgtgaagcg ggcgctggaa
     8521 gagccggtgc gccagattgc caacaacgcg ggccatgaag ggtccgtggt ggtggaaaag
     8581 gtgaggaact cgtcgggatc cttcggcttc aacgcggaca cggaagttta tgaagacctg
     8641 acggaagcgg gcgtcatcga tcccaccaag gtggtgcggt ttgccctgca gaacgcggcc
     8701 agcgtgtcgt ctctgctgct gaccaccgag gccatggtgg ccgaaaagcc caagaagaag
     8761 gaagcggctc ctcccatgcc cggcggaccc ggcatggaag acatgtacta gaaggccgcc
     8821 cgccttcgtc ccttcacggg tggagtcgaa gccggacggg tcctcgaccc cgcgaagggc
     8881 agtattcgtc tcgatagaaa cccccgggag gaacccgcct cctctcgggg gttcttgtgc
     8941 ccggttccgg gttcgcagcc gcccggcgct tgcgggtgtc gcccatctct ccaaaatcgc
     9001 cgcttccagc acggtgaggc cgcccccacc ccacggggct ttctcgggga gcggctcggg
     9061 aggctcgacc cagcggcgcc cggacgcttt gcctctcttc cggggggtcg ccgtccgccg
     9121 ccctttcgcc tcctccctgg cggccgcttg tgccccgctt acgggggtcg gcccctcttc
     9181 cgagttgaca acttcaccgc catcctgcat agtttgcccc ctctgggact cacggaaaaa
     9241 gcgtttccat ccaatattca gcatcagtac ggcacaagga ccgccaggcg gaaccaaccc
     9301 cttctcccga gagcctcttt ccgtttttgc cttggggagc gggaactcca gggttccggg
     9361 agggttttga tttcgttccc gggtgcgact caaacgcctt gatgcggagg caacgggaac
     9421 ccgtggaagt tccttggatc catccgcgcc cgattctgca ctcccaaccc acaaaccggt
     9481 ggttcggacg gagcgcgcgg cgtgaatcaa tccccggtgc aagcccagac attttcggac
     9541 ccggtccggg agttcaatct tcttggggcc cgggggtgcc gacagcgggt cgtgggcttc
     9601 cccctcagcg ccgtgacgct gggtcgcccg ggatcggcct cccgaggacc gggatgaaac
     9661 acagggctcg agaaccccgc cgctccccgg gcaggatgga cgcccgggga taagcgggtc
     9721 ataaagcatt ccaatatagg ggagattgta gacatccgat ccgcagacgg gcgttgcggg
     9781 ggcggaccag gggcaatatg ttggaatccg tcaccgaatg tcggaagaga gagtacctct
     9841 tcaggctaaa aaacctcatt cttggcacga aagaatggag aaacggaacc cgacggccaa
     9901 acccggatag gtcgggtgct gatggtaggg agctggatga gtccaacggt ttttcgccac
     9961 aagagttttc ggtttcactt cttgtctcgt gaagaccacg catgcacatt cacgttacct
    10021 gtgcccatgg ggaggctcaa tttcggatcg aaccagggat actgttgaca agaagctccg
    10081 gtttgacgga atccgaactg cggctcttgc ccagtgtcat tgaggagcaa gtggatgaaa
    10141 tcaggagcgt ccggcacgcc catttgggac atggaggtca cgaacatctc caaacacggc
    10201 ttttgaatcc ttctccggga agaggaggtt tttctgcctt ttgatgactt cctgtggttc
    10261 gggtccgcgc cggtttccgc catattcagg gtagagatgg tgggaccggg gcatccgtat
    10321 tggccggacc tggacgttga tctgagcgtg gagtccatta agcacccgga aaggcatcct
    10381 cttatctcga agtcaatgcc gaacaagccg acgagcaacc aagtatgagg atcccgtaag
    10441 aagtcgttct tttcggaacg gcacctaaca gggtgttgaa aaagcaacaa atatgcgtgc
    10501 tttattttca ggtttgat
//
LOCUS       DAOOVC010000136        10432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4099_length_10432_cov_6.314831, whole genome shotgun sequence.
ACCESSION   DAOOVC010000136 DAOOVC010000000
VERSION     DAOOVC010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4099_length_10432_cov_6.314831"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1132
                     /locus_tag="PLM79_14305"
     CDS             149..1132
                     /locus_tag="PLM79_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOV87532.1"
                     /translation="MRKGKREGSRNLLVCVVVVVTAGCLCPGLAGNAQAYDLPAVNLG
                     FTSFLDGGPPSGPGLYLTQYLQYYHSGSFNDRNGDKLPLPDPDLDVWVSLTQLVYQSN
                     REILLGGKWGLDLIVPIVGFDLGYAFPGPFPQDNGAGLGDILIGPFLQWDPIMGPRGP
                     LFMHRIELQMILPTGKYDSDRELNPGSNFFSFNPYWAGTFFITPRWTVSGRFHYLWNA
                     ENGDPNRGYVGADDTQAGQAFHFNYATEYEVIPQRLRLGINGYYLKQITDTQMDGHDV
                     PDSKEQVFAVGPGALFHITKDTHLFFNIYFEMAAENRTEGNRFNLRLVHHF"
     gene            1270..1686
                     /locus_tag="PLM79_14310"
     CDS             1270..1686
                     /locus_tag="PLM79_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV87533.1"
                     /translation="MKVHEFMVSKIEFIDSDRTVYDAVEIMVDKRIRSLVIRVGGKSD
                     REAVDGVVTARDIVFKVLAKGLNPKAVKVADIASRPLACVEKDMDFDDAATLMEKANI
                     ARLFVCDGEKVVGVVSLLDVMAAELIMWARGDYDSR"
     gene            1673..2302
                     /locus_tag="PLM79_14315"
     CDS             1673..2302
                     /locus_tag="PLM79_14315"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HOV87534.1"
                     /translation="MILDKILSLGKKERRVTEEIRRHIELLCDACDKFRCALEIGDSD
                     LMREVSELEREGDSVRRRVISHIYDGAFLPYIRPDLCRFVESVDEVFDALADTTIYYG
                     EARVPEAIRGECIRVALLNHRMSDLLRLTYEAMLAGEDLREKTLAIRIFEKKIDDMKF
                     TLRSEARKIPVENYWVGKGLSDFILGVTSISDLIEDASDQLEVLSVILR"
     gene            2451..3413
                     /locus_tag="PLM79_14320"
     CDS             2451..3413
                     /locus_tag="PLM79_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOV87535.1"
                     /translation="MSGIFLGWSLGANHTGNVFGTGVATGTVKYRTAIILTAIFVFLG
                     SYLEGPKCMKTVGALSTLTPIEAFYCALAAGLMMSILTFLAFPGSASQAIIGAVLGAG
                     VLSHTADFTKLYKIVTCWAFTPVFAIFASYSLYHILGYALERTRISMTRRNLIYSIGT
                     LLTGCYGAYCLGSNNVANVTGVYVGSGLLDARTAALIGGISIAAGVLTYSRKVMMTIG
                     KGIVPLDPFSALVSVLASALCLHVFTQIGVPVSSSQAIVGSVVGIGLAGDVQTVSLKM
                     LGRIALGWVLAPTTAGILAYLFIRFDLLALLKSWHFFFLQFHGM"
     gene            3473..4087
                     /locus_tag="PLM79_14325"
     CDS             3473..4087
                     /locus_tag="PLM79_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87536.1"
                     /translation="MVPLKRIWKLYIVYATALLTLVAVTGFVLHGQLKYRLMNQVQEQ
                     LFTLARVLAKSLPDDLDNSAHLHSWCRDYRQVSHLRITVIQSNGRVIGDSEDEIVPED
                     SHLDRPEVAGALVEGSSVAVRFSITMDTDMLYAAYLVEGRQVILRLAMPMSMMKQIEN
                     EVMLFFVIAVYLAPLLAIGIPFAFGKFVLSREEPAERPPRRSRC"
     gene            4107..4535
                     /gene="arfB"
                     /locus_tag="PLM79_14330"
     CDS             4107..4535
                     /gene="arfB"
                     /locus_tag="PLM79_14330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOV87537.1"
                     /translation="MTIRINEQVSIPEQELHFTASHSSGPGGQNVNKVSSRVTLWFDV
                     GDSPSLTPEQKGLIIRRLRNRINKDGVLRIDSQQTRSQAANRELAVERFSELMQGALR
                     RVPTRKKTRVSRAAKLRRLQEKKKRSTMKKERSRKVSFED"
     gene            complement(4630..7623)
                     /locus_tag="PLM79_14335"
     CDS             complement(4630..7623)
                     /locus_tag="PLM79_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HOV87538.1"
                     /translation="METDLETRIHKTGTGLHQMMGEGTPSFFHRAYWTSKMLEWCMQN
                     ESFKTELFRFVDVFPSLSRPETIAGHLHEYFSRPGLDLPRPVRKILEYTSPSTPTGKL
                     IAGRVARVLSTMAGQFMVGESPKKVLGVLERMRSHGIAFTVDLLGEAVVSEAEAGEYV
                     HRYLEWIELLDRVQKDWKPLGTSGGDLDWGRSPKINLSIKTSALYSQMNPCSFEHSVH
                     MAKGRLRPIVHRVMRAGGSITLDMEQVALKNLTLALYRSLREEPEVREYPHTGIVIQS
                     YLRDSEQDLRSILDWAREKDFRLTIRLVKGAYWDSEVITARQNNWPIPVFTRKHETDA
                     NFEKLARIILENHQWVTLACASHNLRSIACVMETARELRVPEDRMEFQLLYGMGESIQ
                     RALSRAGVPLRLYSPVGNLVQGMAYFVRRLLENTANESFLRLSFTEGVPMEDLLKNPR
                     DLAGEGGTHAPAPWELPAFEPVEDRWPPFENEPPLDWTREENRNGFRAALDRVRKSFP
                     REVPLFIGGHYVETERKILSVNPNDPEEIVGEVSSGGRDEGERAVRAARGSFPGWRDT
                     DPVVRAGFLFRAAAQTRKRRLELAALQVFEVGKSWTEADADVCEAVDFLEYYGREMIR
                     LATPRRMGRVPGELSHLWYEPRGVAAVIAPWNFPLAISMGMTSAAVVTGNPVVYKPAS
                     QSPVIGSMIAEIFREADLPPGVFNFVPGPGSELGDVLATHPDVALVAFTGSKAVGLEI
                     IEKAARVPAGSACVKNVIAEMGGKNAVIVDADADLDEAVVHIVRSAFGYQGQKCSACS
                     RLIVLEENCERLLERLKAAVQSLHLGPPEDPKNFVGAVIEPTARERILDYIEIGTVEA
                     KLFLRRDLEGARGFFAPIAVFTEVNPRSPLAREEIFGPVLAVLRVKDFEEALRVANST
                     PYALTGAVFSRSPRNIERARKAFRTGNLYINRGCTGAVVGRHPFGGFGLSGVGSKAGG
                     PDYLLQFMVPKNACENIVRRGFAPW"
     gene            complement(7680..8453)
                     /locus_tag="PLM79_14340"
     CDS             complement(7680..8453)
                     /locus_tag="PLM79_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87539.1"
                     /translation="MAAIPPKTLAKTLTYILVTAPGEFGLFWNPDGTMPWKELYWALQ
                     EDEALRFVRESHVREIRYLGIELPFDLDGSVLRWKASRALPPYPLELHLLPRRLYFAC
                     RPRQLPRIRENGLEAGARPYLPLTADRQAALRIGRRRDPESVVLEIITDEAARAHVPI
                     YRADHGLYLANSIPARFIHFPLLRREDGTEAVTGKKKKTGKVSAGLSATPGSYLLSPR
                     DLGTAGGNFDREPKQPQKKGKGKSGWKRSLGRERQKRTP"
     gene            complement(8495..9409)
                     /locus_tag="PLM79_14345"
     CDS             complement(8495..9409)
                     /locus_tag="PLM79_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87540.1"
                     /translation="MLTNVTGIFYHPSFSRRSYLTQGSRLRHFPRVIEPLLRDPRFRL
                     FESPMVPEDLLLRVHSAQLIREVDRDPLCSTGRRSAGGVVLAGETVARGEIRNAFAFI
                     GAGGHHSGRTFFGGSCCFNDVVLAVQHLRDLDLLHRFAILDTDAHHGDGTRQLVEADP
                     DVLHVCVCHDTYESRDGTKVDVPAPSPYDRFSNREVSSDVDFLYADAALSAFEPRVRR
                     FRPDLIFWYFGFDTHRGDYGDLGLSGTCYTIIADRMKELAEELCSGRLEVVLGGGSRS
                     DIARDVIPPILGILGGVSIPPSSAFSSS"
     gene            9498..9572
                     /locus_tag="PLM79_14350"
     tRNA            9498..9572
                     /locus_tag="PLM79_14350"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9530..9532,aa:Gly,seq:gcc)
     gene            9621..>10432
                     /locus_tag="PLM79_14355"
     CDS             9621..>10432
                     /locus_tag="PLM79_14355"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOV87541.1"
                     /translation="MKKTISSHLVSRNPGRKRSVKEALKQLYSLFHFKDQVLITVDPD
                     PDAIGSALALKRLLWHKVQSTTIGIIRPVRRLNNRTMVRLLKLPLTLLKEKHLSQYDK
                     FLLVDGQPCHNPFFAEISYTAVLDHHPLTGFKAEFTDIRPDYGATSTILTEYLRAAGI
                     KPSQTLATTLLYGIKTDTRSFERHTRVEDIGAFRYLYPLANHNVLRKIEISDLSLSDL
                     KYFHKAVDRKHVVRDRIFVHMDEVPTADLLVIIGEFFLKVHDISWSITSGIV"
BASE COUNT         2122 a         3145 c         3047 g         2118 t
ORIGIN      
        1 tgaggggatc cccgggcgga ttccgtgagc aatccacccg ggaggagagg gcgagagagg
       61 cccggcgaca acccaccatc cgctggttgg gcgtatgccc ggttgaggga tctcatccgg
      121 gccatggaca tgggaaagga ggaacgggat gaggaagggg aaaagagaag ggtcgaggaa
      181 tcttttggtt tgtgtggtgg tcgtcgtgac ggcaggttgc ctttgcccgg gcctggcggg
      241 gaacgcccag gcctatgatt tgcccgcggt caacctggga ttcacgagtt tcctggacgg
      301 aggtcccccg tcgggacccg gcctgtacct gacgcaatat ctgcagtact accactcggg
      361 cagtttcaac gaccggaacg gggacaagct tcctttgccc gacccggacc tcgacgtctg
      421 ggtgagcctc acgcaactgg tctaccagtc caaccgggaa atactgctgg gaggaaaatg
      481 gggtctggat ctcatcgtcc ccattgtggg ctttgatctg ggatacgctt tcccgggtcc
      541 gttcccccag gacaacggag caggactggg ggatatactc atcggtccct ttctgcagtg
      601 ggatccgatc atgggccccc ggggacctct tttcatgcac cgcatcgagc tccaaatgat
      661 cctccctacg gggaaatacg acagcgaccg tgaactgaac cccggcagca atttcttttc
      721 cttcaatccc tactgggccg ggaccttctt catcacgccc cgttggaccg tttccggccg
      781 tttccactac ctgtggaacg cggaaaacgg cgaccccaat cgcggatatg tgggggccga
      841 cgacacccag gcgggccagg ctttccactt caattacgcc acggagtacg aagtgattcc
      901 ccagaggctg cgactgggca tcaacgggta ttacctaaag cagatcacgg acacccagat
      961 ggatgggcac gacgtcccgg acagcaagga acaggttttc gccgtcggtc ccggagccct
     1021 tttccacatt accaaggaca cgcacctgtt tttcaatatc tatttcgaga tggctgcgga
     1081 aaaccgcacc gagggaaacc ggttcaattt gaggctggtg catcatttct agcgggacgt
     1141 cgccgggtgg cgatatccca cggcgcctga gggtgaagcc aattttggcg gcgttgtttc
     1201 ggggttgccg agtcgggact ctttccggaa tgtgcactat ggcgattcgg cggattgcgg
     1261 gggtatgaga tgaaagttca tgagtttatg gtcagcaaga ttgaattcat cgattccgac
     1321 aggaccgtct acgatgcggt ggagatcatg gtggacaagc ggatccgttc cctggtgatc
     1381 cgcgtgggag gaaaatcgga ccgggaggcc gtggacgggg tcgtaaccgc ccgggatatc
     1441 gttttcaagg tgttggccaa aggattgaat cccaaagccg tgaaggttgc ggacatcgct
     1501 tcgcgccccc tcgcctgtgt cgaaaaagac atggatttcg acgacgccgc aaccctcatg
     1561 gaaaaggcca acattgcccg gttgtttgta tgcgacggag aaaaggtggt gggcgtcgtt
     1621 tcgttgctgg atgtaatggc ggcggaactg atcatgtggg caagaggtga ctatgattct
     1681 cgataaaatc ctgtccctcg gcaagaagga gaggagggtt accgaggaga tccgcaggca
     1741 catcgagctg ctttgcgatg cctgtgacaa gttccgctgt gccttggaga tcggggactc
     1801 ggacctcatg cgggaggtga gcgaactcga acgggaaggg gactccgtcc gcaggcgggt
     1861 catctcccac atctacgacg gtgctttcct gccctacatc cggccggatt tgtgccgatt
     1921 cgtggagagt gtcgatgaag tgttcgatgc ccttgcggat acgacgatct attacgggga
     1981 agcccgagtg cccgaagcca taaggggaga gtgcatccgg gtggccctcc tcaaccatcg
     2041 catgagcgac ctgcttcgac tgacctacga ggccatgctg gctggggaag atctccggga
     2101 gaaaaccctg gccattcgga tctttgaaaa gaaaatcgac gacatgaagt ttaccctgcg
     2161 aagcgaagcc cgcaaaatcc ccgtcgagaa ctactgggtc gggaaagggc tgtcggattt
     2221 cattctgggt gtcacctcca tcagcgacct catcgaagac gccagcgacc aactggaggt
     2281 gttgagtgtc atcctccgat gatgtctttg ccgatcaatc ctctttgccg tagcccgcaa
     2341 cgccgcctgc tcccgccggg ctcgagcgca accatccccc gccgagggaa tggaaatctt
     2401 tcccgcccgt tccggccgac cttcacggtg cttcaactat gtggatgact ttgagtggga
     2461 tattcctcgg ctggagtctg ggcgccaatc acaccggcaa tgtttttggg accggggttg
     2521 ccacgggcac cgtcaagtac cgaacggcca tcattttgac cgcgattttc gtcttcctgg
     2581 gatcatacct ggagggcccc aaatgcatga agaccgttgg ggcactctcg accctcaccc
     2641 ccatcgaagc cttttactgc gccctggccg cgggcctcat gatgagcatc ctgaccttcc
     2701 tggcctttcc cgggtcggcc tctcaggcca tcattggggc cgtcctgggg gcaggggtcc
     2761 tatcccatac ggcggatttc accaagctct acaagatcgt tacctgctgg gccttcactc
     2821 ccgtctttgc catttttgcc agctacagcc tctatcacat tctgggttac gcactggaga
     2881 gaactcggat cagcatgacc cgccgaaacc tcatctattc catcggaacc ctcctcaccg
     2941 gctgttacgg agcctattgc ctgggttcca acaacgtggc gaacgttacc ggggtttacg
     3001 tgggttccgg tctcctggac gcccgcaccg ccgccctcat cgggggaatc agtatcgcgg
     3061 ccggcgttct gacttacagc cggaaggtca tgatgaccat cggcaaggga attgttcccc
     3121 tggatccttt ttccgcgctg gtttcggtgt tggccagcgc cctctgtctt cacgttttca
     3181 cccaaatcgg cgtccccgtg tcttcctccc aggccatcgt gggctccgtg gtgggaatcg
     3241 gcctggctgg agacgttcag accgtgagtc taaaaatgtt ggggaggatc gccctggggt
     3301 gggttctggc acccactacc gccggaattc tggcttactt gtttattcgc ttcgacctgc
     3361 ttgccttgct caagagctgg catttctttt ttctgcagtt ccacggcatg tgagagggtt
     3421 acggccgcct gcctaaaacg gcttgttggc gaggcaccat gaggagggcc ctatggttcc
     3481 gctcaaacgc atctggaagc tctatattgt ctatgccacc gcactgctga ccctggtggc
     3541 ggtgaccggt ttcgtcctcc acggtcagct caagtaccgc ctcatgaacc aggtgcagga
     3601 acagctgttc accctggcgc gggtcctggc caagtccctg cccgacgacc tcgacaactc
     3661 cgcccacctc cattcctggt gccgggacta ccgacaggtc tcccatctgc ggatcaccgt
     3721 catccagagc aacggtcggg tgattggaga ttccgaggac gagatcgttc ccgaagattc
     3781 tcacctggac aggccggagg tggccggcgc gcttgttgaa ggttcctccg tcgccgttcg
     3841 cttcagcatc accatggata cggacatgtt gtatgccgcc tacctggtgg agggtcggca
     3901 ggtgatcctt cgactggcga tgcccatgag catgatgaaa cagatcgaaa acgaggtgat
     3961 gctttttttc gtcatcgccg tctacctggc tcccctgttg gccatcggca tcccctttgc
     4021 cttcggcaaa ttcgtcttga gccgggagga acccgcggaa cgtccgccga gacgttccag
     4081 atgctgaaag aaaaggggac atccccatga cgatccggat caacgagcag gtttccattc
     4141 ccgaacagga gctgcatttc acggcatctc acagcagcgg gccgggcggt caaaacgtca
     4201 acaaagtctc cagccgcgtc accctgtggt tcgacgtagg agattcacca agcctcaccc
     4261 cggaacagaa gggcttgatc atccgcaggc ttcggaatcg gatcaataag gacggggtgc
     4321 tcaggattga ttcgcagcaa accaggagcc aggcggcaaa ccgggagctt gccgtggaac
     4381 gattctccga attgatgcag ggggctctca ggcgggtgcc gacaaggaag aaaacgcggg
     4441 ttagccgggc agcaaaactc cgccgccttc aggagaaaaa gaaacgcagc accatgaaaa
     4501 aggagaggtc ccggaaggtt tctttcgagg actaggcacc ccaaatattg aacccgggtt
     4561 tgcgcggtgg tgaaccgccg acggcttcca cgcatgggca tttgccggtc ttcccgggat
     4621 ggaaagggcc taccagggag caaatcctcg ccggacgatg ttctcgcaag cgtttttcgg
     4681 caccatgaat tggagcaggt aatcgggtcc gcctgccttg gaacccaccc cggagagccc
     4741 gaatcccccg aaggggtgcc tgcccaccac ggctcccgtg caaccccgat tgatgtagag
     4801 gttccccgtg cgaaaggcct ttcgagctcg ttcgatgttc ctggggctcc gggaaaaaac
     4861 cgcccccgtc agggcgtagg gagtgctgtt ggccacacgc aaagcctctt caaaatcctt
     4921 gacccggagg acggccagca cgggaccgaa gatctcttcc cgggccaggg gagaacgggg
     4981 gttcacttcc gtgaagacgg caatgggagc gaaaaagccc cgagcaccct cgaggtccct
     5041 ccggagaaaa agcttggcct ccacggtgcc gatttcgatg taatccagga ttctctcccg
     5101 ggccgtgggt tcgatgacgg caccgacgaa attctttggg tcttcggggg gtcccaggtg
     5161 gagactctgg accgcggcct tcagtctttc caggagccgc tcgcagtttt cctcgaggac
     5221 gatcaggcgg gaacaggccg aacacttctg accctggtat ccgaaagcgg atctcacgat
     5281 atggaccacc gcctcatcca ggtccgcatc ggcgtctacg atgacggcgt ttttcccgcc
     5341 catttccgcg atgacgttct tcacgcaggc gctgcctgcc gggacccgtg cagccttctc
     5401 gatgatttcc aggcccacgg ccttgctccc cgtgaaggca acgagggcca cgtccgggtg
     5461 agtcgccaaa acatccccca gttcgctacc gggtcccggc acaaaattga acacccccgg
     5521 cggcagatcc gcctcccgaa agatctcggc aatcatggag cctatgaccg gtgattggga
     5581 tgcgggtttg tagacgacgg ggtttcccgt caccacggcc gcagaagtca ttcccatgga
     5641 aatggccagg ggaaagttcc acggggcaat gacggcggca accccccggg gttcgtacca
     5701 aaggtggctc aattccccag ggacgcgtcc catgcgccgg ggagtcgcca ggcgaatcat
     5761 ttcgcgtccg taatattcca gaaaatcgac ggcttcgcac acatccgcgt cggcttcggt
     5821 ccaggatttt cccacctcaa agacctggag agccgcaagt tcgaggcgcc gcttgcgggt
     5881 ttgcgccgca gcccggaaga gaaatccggc acggaccacg gggtccgtat ccctccagcc
     5941 cggaaaggac ccccgggccg cccgcaccgc tctttccccc tcgtctcttc cccccgacga
     6001 cacctctccc acgatttctt ccggatcgtt gggattgacc gagaggatct tcctctccgt
     6061 ctcgacgtag tggccgccga tgaaaagggg aacctcccgg ggaaaggact ttcgcacccg
     6121 gtccaaggcg gctcgaaacc cgttgcggtt ttcctcccgg gtccagtcca ggggcggctc
     6181 attttcaaag ggcggccacc gatcctccac gggctcgaag gcgggaagct cccagggagc
     6241 cggtgcatga gttcctcctt ccccggcaag gtcccggggg ttttttagaa ggtcctccat
     6301 gggcacccct tcggtgaagc tcagccgcag aaaggattcg ttggcggtgt tttccaggag
     6361 ccttcgaaca aagtaggcca tcccctggac caggtttcct acgggactgt acaggcgaag
     6421 aggcaccccc gcccgggaaa gggcccgttg aatcgactcc cccattccat agagaagctg
     6481 gaattccatt cggtcctcgg ggacccgcaa ttcccgggcc gtctccatga cgcaggcgat
     6541 ggaacgaaga ttgtgagagg cacaggcgag ggtcacccat tggtggtttt ccaggatgat
     6601 tcgagccagc ttttcgaaat tggcgtccgt ctcgtgcttg cgggtaaaga ccgggatcgg
     6661 ccagttgttc tggcgtgcgg tgatcacttc cgaatcccag taggcccctt tgaccaggcg
     6721 aatggtcaga cggaaatcct tttcccgcgc ccaatcgagg atcgaacgca ggtcctgttc
     6781 gctgtcccgc aggtaggact ggatgacgat ccccgtgtgg ggatattccc ggacttcggg
     6841 ttcctcgcgc aggctgcgat agagggccag ggtaaggttc ttgagcgcca cctgttccat
     6901 gtccaacgtg atggacccgc cggccctcat gaccctgtgg acaatcgggc gaagccgccc
     6961 cttggccatg tgaaccgagt gttcgaagga gcagggattc atctgggagt acagggcgga
     7021 ggtcttgatg gagaggttga tcttggggga acggccccag tccaggtccc ccccggaggt
     7081 ccccaacggc ttccagtcct tttgcacccg gtccaggagc tcaatccatt ccaggtaacg
     7141 atgcacgtac tccccggctt ccgcctcgga aaccaccgct tcccccaaca ggtccaccgt
     7201 gaaggcaatc ccgtgggagc gcatccgttc caaaaccccc aggacctttt tggggctttc
     7261 ccccaccatg aactgcccgg ccatggtgga gagcacccgg gccaccctgc cggcaatcaa
     7321 tttcccggtg ggtgtggacg gggaagtgta ctcgaggatc ttccggacgg gcctgggtaa
     7381 atcaaggccg ggccgggaga agtattcgtg aagatgcccg gcaatggttt cgggtcgcga
     7441 cagggaagga aagacatcca caaaccggaa caactcggtc ttgaacgatt cgttttgcat
     7501 gcaccactcg agcatcttgg acgtccagta tgctcgatga aaaaaggaag gggttccctc
     7561 gcccatcatt tgatgcagtc cggttccagt cttgtgaatc ctcgtctcca ggtccgtctc
     7621 catcgtgacg ctctcccttt ggttgaatca aacggttgcg ttgcacccga tatgaatcct
     7681 cacggggtgc gcttctgtct ttcgcgcccg aggcttcgtt tccagccgct cttgcctttc
     7741 cccttctttt ggggctgttt gggttcccgg tcgaaattgc cccccgcggt ccccaagtct
     7801 cgaggggaca ggagatagga tcccggagtg gcggacaagc ccgccgaaac cttcccggtt
     7861 ttctttttct ttcccgtcac cgcctcggtc ccatcctccc ggcgcaacag gggaaaatga
     7921 atgaaccggg caggaatcga gtttgccagg tacagcccgt ggtccgcccg gtaaatcggc
     7981 acatgggccc ttgcggcttc atcggtgata atctccagga ccacggattc ggggtcccgg
     8041 cggcgcccaa tgcgaagggc cgcctgccgg tccgcggtca acggaaggta gggacgagcg
     8101 cccgcctcca ggccgttttc ccggatacgt gggagctgcc ttgggcggca ggcaaaatag
     8161 agcctccggg gcaggagatg taactccaaa gggtaggggg ggagggccct cgaggccttc
     8221 caccgcaaga cggaaccatc caaatcgaaa ggcagttcga tccccagata ccggatctct
     8281 ctcacatggg attcccgaac aaaccgaagg gcttcatcct cctgcagagc ccagtacagt
     8341 tctttccacg gcatggtccc gtcggggttc cagaaaagac cgaattcgcc cggggcggtt
     8401 accaggatgt aagtaagggt ttttgctagc gtttttggcg gaattgctgc caattccttg
     8461 tctcctttag aaaaactccc gcgggagtga acgatcacga ggaggaaaaa gccgaggagg
     8521 gcgggatgga aacccccccg aggattccga ggatcggggg gatgacatcc cgggcaatgt
     8581 cgctgcggga cccgccgccc aggaccacct cgagacgccc ggaacaaagt tcctcggcca
     8641 gttctttcat acgatccgcg atgatcgtgt aacaggtgcc gctcaacccc aggtctccgt
     8701 agtcgccgcg gtgggtgtcg aagccgaaat accaaaaaat gaggtccggt cgaaaccggc
     8761 gaacccgggg ctcgaaggcc gaaagggcgg cgtcggcata gaggaaatcc acatccgaag
     8821 acacttcccg attcgaaaat cggtcgtagg gactgggagc ggggacgtcc actttggttc
     8881 cgtcccggga ttcgtaggta tcgtggcaga cgcagacatg aaggacgtcg gggtccgcct
     8941 ccacaagttg gcgtgtgccg tcgccgtgat gggcatcggt gtccaggatc gcgaaacggt
     9001 gaagcagatc caggtcccgg aggtgttgaa cggccaacac gacgtcattg aagcagcaag
     9061 agcccccgaa gaaggttcga ccggaatgat gccctccggc cccgatgaag gcgaaggcgt
     9121 tccggatctc tccccgggcg acggtttcgc ccgccaggac cactccgccc gcggaccggc
     9181 ggccggtcga gcacaggggg tccctgtcga cctcccggat gagttgggcc gagtggactc
     9241 ggaggaggag atcttcggga accatggggg attcgaacag ccggaaacgg ggatcccgga
     9301 gcaagggttc gatgaccctc gggaaatgcc ggaggcgcga gccctgggta aggtagcttc
     9361 gtcggctgaa gctgggatga taaaaaatgc ccgtgacatt agttaacata acatatctta
     9421 tcggacattg tagtgtaccc gggttttttt ccttgacttt gttcatgcca ttcggtaact
     9481 attcccttcg cacgcgagcg ggaataactc agcggtagag tgcaaccttg ccaaggttga
     9541 agtcgcgggt tcaaatcccg tttcccgctc cacccctttt cccccatcaa gaaccctatc
     9601 gccccctggc cagcctgttc atgaaaaaaa cgatctcttc ccatcttgtt tcccgaaacc
     9661 ccggaagaaa acggtccgtc aaagaggccc tcaagcaact ctactccctt tttcacttca
     9721 aagaccaggt gctcatcacc gtcgatcccg accccgacgc catcggttcg gccctggccc
     9781 tcaagagact cctctggcac aaggtccaga gtaccaccat cggaatcata cgccccgtcc
     9841 gaaggctcaa caaccgaacc atggtccggc tcctcaagct ccctctcacg ctcctcaagg
     9901 aaaaacacct gagccaatac gacaagttcc tcctggtgga cggtcagccg tgccacaatc
     9961 cctttttcgc cgagatttcc tacaccgccg tcctggacca ccaccccctt acgggattca
    10021 aagccgaatt cacggacata aggcccgatt acggagccac ctccaccatt ctcacggaat
    10081 acctccgggc cgcagggatc aaaccctccc agaccctggc aaccacgctg ctctacggca
    10141 tcaagacgga cactcgaagc ttcgagcggc atacccgggt cgaggatatc ggggcctttc
    10201 gctacctgta ccccctcgcc aaccacaacg tcttgcgaaa aatcgagatc tccgatcttt
    10261 ccctctccga tctcaagtat ttccataagg ccgtggaccg gaagcacgtg gtccgagacc
    10321 ggattttcgt gcacatggat gaggttccca cggcggatct cctggtcatc atcggcgagt
    10381 tcttcctcaa agtccacgat atttcctgga gcatcacttc cggcattgtc ca
//
LOCUS       DAOOVC010000137        10222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4202_length_10222_cov_6.154618, whole genome shotgun sequence.
ACCESSION   DAOOVC010000137 DAOOVC010000000
VERSION     DAOOVC010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4202_length_10222_cov_6.154618"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLM79_14360"
     CDS             <1..1029
                     /locus_tag="PLM79_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87542.1"
                     /translation="LPVFLAFTYVWTFPWPAVLPPALVRGFKRLRLEGLRKSPDLLPL
                     VWIFVVLGLFSASQSRLEYYALPCLPAFALLMGKYWEELSRDTAQDRSRKAAGIALGI
                     LTVLLAMAALAAMIILGPQKEVIFHAFQQNYPDAGWVEGPVQLSILDRIHFPTIATLL
                     GTAVCMGLATAALFRGRTVWSVRILAGMMIPVFLLIHWGFRVMEPYHSTRAMAEIVRQ
                     AATPDHIVVFTEPHEYMWVGGITYYTKRLVHILKDPRYDHVSTRRREPPQRFLDNTEL
                     TALWTSDKPVLLVADVTRRDVHPILAAAGSVEVVARIGNLVTYGNQGGSTPAQPLPGP
                     SHWPSPSQ"
     gene            1136..1978
                     /locus_tag="PLM79_14365"
     CDS             1136..1978
                     /locus_tag="PLM79_14365"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HOV87543.1"
                     /translation="MAQLPSSDRADTPREPDGVVIKPPVLFIRTFFRIARAILSILMA
                     VGAAVAIYGARLGMESKFSENWPSVSGIIVTSRVERPPGAEAGGLQAMIVYEYSVAGK
                     KYKGNRVSFNQSSGFAPDDPRKLTARYPRNASVRVYYDPGAPQKSVLEPGFMAASLIP
                     VGIGILLLGIGLAGTRSIRAKEKTFEQEEGLSAPEKSAEPPQPQPVPSVSPGQKRPGS
                     KIPVKKPGWVYPSRQPSEPGPLPPPLPGTTESPLHKLLRYALLAWLVVMLCLAFLRLD
                     WFKS"
     gene            2068..2784
                     /locus_tag="PLM79_14370"
     CDS             2068..2784
                     /locus_tag="PLM79_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOV87544.1"
                     /translation="MTSRIESATSRPAVIFDMDGVLVDSYQAHFESWRRLAEECGRSL
                     TRDEFSSLFGRTSGDILDRVWGAPPPLGERKEMEERKERYYRDILEKEFKPMDGAADL
                     AQALLEHGFLLAVGSSGPPENIALVVHKLGLTGHIPVQITGRDVTRGKPDPQVFLLAS
                     RQLGVRPGDCAVIEDAPAGIAAALAAKMVAVGLAGTVPIEKLRHAHRVVGSLRELTPQ
                     SIARLIREPRAGHQCPPSTR"
     gene            complement(2814..3611)
                     /locus_tag="PLM79_14375"
     CDS             complement(2814..3611)
                     /locus_tag="PLM79_14375"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HOV87545.1"
                     /translation="MSLPAKLRMLPPWLSLSLIIGVVVGFSVAGLLVVRRFIPHHVMK
                     YHSDVTGYIFTTLGLVCGVMLALVIYVVWVQYNNDRVNAGMEAEQTLNLMRGLRLHPN
                     REQAAQVRKALVEYARAVHEKEYPAMRRMEYSGAADEAFGAVWVSLKLMDPRTRGETA
                     VYSEIPGHMDQLARHRFERLMAAHEELPPVRRLSVILEGIVPPAFCRFFGSENFPAHI
                     VMTAMPAFLLAVLIYMIIRLDRPYQSEDHVRPLRYETVLDRISTHER"
     gene            3891..5378
                     /locus_tag="PLM79_14380"
     CDS             3891..5378
                     /locus_tag="PLM79_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV87546.1"
                     /translation="MEATCSIRTSFCGSTGDARREALYYHDGRQDLRGTFSVLSTIPE
                     IERQLPMTSRTFETLMAAKQNLENILDNLMEGLIAHDTERRILFFNRAAEAITGYSRD
                     EVLGRDCHQVFGGPLCGSRCSFCNGPVESLSRLAYSLTVPTKSGESRRIEMSVTGMFD
                     ETGGFMGVLAAFRDVTDLIGLRTRLGEVHRFSGIIGRDHQMLQLYEQIRELALNDFPV
                     HIRGETGTGKELVANAIHNESHRGGGPFVPVNCGALPEGLLESELFGHDRGAFSGAIR
                     DKKGRFELAHKGTLFLDEVAELPKSMQVKLLRVLQQRAFERVGGEKTIRVDVRIISAT
                     NRDLKREMERGNFREDLYYRLNVVPLYLPSLRERKNDIPLLVAHFLEEASNRGHRVAR
                     VSPEAMSLLMDHSWPGNVRELQNAVHFALVKTRGCVIEPEHLPMELRKELCSKSRRGP
                     APKLNPASVEAALAASGGNKSRAAKALNVGRATLYRFLSRSGPVS"
     gene            5810..6016
                     /locus_tag="PLM79_14385"
     CDS             5810..6016
                     /locus_tag="PLM79_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87547.1"
                     /translation="MRLCDENLLKTLELTDRMVEIADQGDMAREDPGCGVLYGVLRDA
                     AFKIRKLAEDERSVHMRKGKWRQD"
     gene            6123..6623
                     /locus_tag="PLM79_14390"
     CDS             6123..6623
                     /locus_tag="PLM79_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOV87548.1"
                     /translation="MANTKTQQNLMEAFAGESQANRKYLAYAKQAEKEGFTQVAKLFR
                     AAAEAETVHAHSHLRVAGGIKQTAENLRDAVAGETHEFKSMYPAMIEAAKEEGNKQAE
                     RTFTYANEVEKVHAELYRKALEKMEAMEPADYYICSVCGYTCENEVPERCPVCNSNSK
                     VFYQVD"
     gene            6828..7274
                     /locus_tag="PLM79_14395"
     CDS             6828..7274
                     /locus_tag="PLM79_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOV87549.1"
                     /translation="MAKIIVIYATRTQQTRKIAELIAEGIRLENVEATVVDVNEATKM
                     TARLSEFDGAVLGSATYHGEMLQPMKTFLFALEKAGLEGKAGGSFGAFGWSGEAPGRI
                     YNTLKHVVRMDMVNGPLMLKSASLGGGIKMAQDYGREIAKKVAAKG"
     gene            complement(7362..7583)
                     /locus_tag="PLM79_14400"
     CDS             complement(7362..7583)
                     /locus_tag="PLM79_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87550.1"
                     /translation="MAYTCKTCGAVAHDPGHLCNPCGDKSRCKFCGTPEVDTTHVCKD
                     KLAQMKYVCRGCGRVAMEEAHLCKPAPIH"
     gene            complement(7715..7966)
                     /locus_tag="PLM79_14405"
     CDS             complement(7715..7966)
                     /locus_tag="PLM79_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87551.1"
                     /translation="MSVDPKVLEQKLKEMYPEIDEHRLALSLCFDPDKDAWIVVLNKG
                     SHELKTHLERKDAEACLEGTQCVYLGVQIGQFVQNFERA"
     gene            8957..>10222
                     /locus_tag="PLM79_14410"
     CDS             8957..>10222
                     /locus_tag="PLM79_14410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87552.1"
                     /translation="MAGRNDGKADRSSPFRPLDFIPHAVVVLRGSKILFANRWTYLVF
                     DWQPDELVGQPLKALCRSEEDCRGLLQELRAWDDSLDVVREGLPCRQKSGKTLICRVS
                     ASFLPKEGRQRLQMLTFDEVTGSAPPDSATVRLSLSQAKEALRKRVRQRTLELQETNR
                     TLRGEIAERKKAEKALRKSERLLADIIEFLPDPTFVVDAKRQIVAWNRAIEDLTGVPG
                     KQMVGKGNYEYAIPFYGTRRPILLDLVFENRHEIEEEYRILERRERVVLGEACFTMTN
                     GRTVCLWGKAAPILDHDGKVIGAIECIRDVTEKKQGEEALRRSEDRFSKAFLSSPTPT
                     LLTVLSEDTILDANESFCRWLGYSPTELIGRTCRDLPLWANAQDQAVFIGDLRERGSL
                     RNEYSRVLTRKGEVRDILVSAEILPTGGED"
BASE COUNT         2059 a         3186 c         3053 g         1924 t
ORIGIN      
        1 cttccggtgt ttctggcctt cacctatgtc tggaccttcc cctggccggc ggttctgccc
       61 ccggccctgg ttcgaggatt caagcggctt cgcctcgagg gcctccgaaa atccccggac
      121 ctcttacccc ttgtctggat cttcgtcgtc ttgggcctct tttccgcttc ccagtcgcgt
      181 ctcgagtact atgccctgcc ctgtctcccg gcttttgcac tgctcatggg caaatactgg
      241 gaggaactct cgagggacac ggcgcaggat cggtctcgga aggccgcggg catcgccctg
      301 ggcatcctta ccgtcctgct ggccatggcc gcccttgcgg ccatgatcat cctgggtccc
      361 cagaaagagg tcatcttcca cgcttttcaa cagaactacc ccgatgcggg ctgggtcgag
      421 ggacccgtcc agctctccat cctggaccgg atccattttc ccaccatcgc cactttgctg
      481 ggtacggcag tatgtatggg gttggcaacg gcggcgcttt tccggggccg gaccgtctgg
      541 agcgttcgga ttttggcggg aatgatgatc cccgttttcc tgctgattca ctggggattc
      601 cgggtgatgg agccttacca ctcgacccgg gccatggcgg agatcgtccg gcaggccgca
      661 acccccgacc acatcgtggt cttcacggag ccccacgaat acatgtgggt cggcggcatc
      721 acgtactaca cgaaaaggtt ggtccatatt ctcaaggacc ctcgctacga ccacgtttcc
      781 acccggcgcc gggaaccccc ccagcgtttt ctcgacaaca ccgaactcac ggcgctttgg
      841 acctccgaca aacccgtgct cttggtcgcc gacgtaaccc gcagggacgt tcaccccatc
      901 ctggcggcgg ccgggtcggt cgaggtagtg gccaggatcg gcaatctcgt gacctacggc
      961 aaccaggggg gatcgacccc ggcccaaccg ctcccgggcc cttcccactg gccttccccg
     1021 tcgcaatagt ggtacaattc gcctcaatcg gacccgggac ccgggggaaa gagcactgaa
     1081 ccccgtcctt cccgcatcct ccggaatcag gtcggcagaa cacaggagtc ctaccatggc
     1141 gcagttgcct tcatccgacc gagcggacac gccccgggag ccggacgggg tggtcatcaa
     1201 gcctcccgtc ctgttcattc ggaccttctt tcggattgcg cgcgcgatcc tctccatcct
     1261 catggccgtc ggggccgcgg tcgccatcta tggggcccgc ttgggcatgg aatccaaatt
     1321 cagcgaaaac tggccctcgg tttccgggat catcgtcacg tcccgggtgg aaagaccccc
     1381 gggggccgag gcgggcggtc tgcaggccat gatcgtctat gaatactcgg tagcgggcaa
     1441 aaaatataaa gggaaccggg tctccttcaa ccaaagcagc ggctttgccc ccgatgatcc
     1501 ccgaaagctc acggcccggt acccgcgcaa tgcgtccgtc agggtctatt acgatcccgg
     1561 agcaccccaa aagtcggtgc tcgaaccggg attcatggcc gcctccctga ttcccgttgg
     1621 aatcggcatt cttctcctgg ggatcgggtt ggccggaacc cgaagcatcc gtgccaagga
     1681 aaagactttc gagcaggagg aagggctctc ggccccggaa aaatccgcgg aaccgcctca
     1741 acctcaaccg gtgccgtcgg tttctcccgg acagaagcga cctgggagca agattcccgt
     1801 caaaaaaccc ggttgggttt atccctcccg gcagccttcc gaacccgggc cccttccgcc
     1861 gcccctcccg gggacgacgg aatcgccgtt gcacaagctc cttcgctatg ccctgctcgc
     1921 ctggctggtc gtcatgctct gcctggcttt tcttcgcctg gactggttca agtcctgaca
     1981 caccgcgccc ccccggcgcg caacggacca ggaagacagg tccctttcgg ggaaaacacc
     2041 ttcggccctt caaggagctc acaggccatg acatcccgca tcgagagcgc aacttcccgg
     2101 ccggcggtca ttttcgacat ggacggagtc ctggtggatt cgtaccaggc ccatttcgaa
     2161 agctggcgac gcctggcgga agaatgcgga aggtccctca cccgggacga gttctcctcc
     2221 ctcttcggcc ggaccagcgg ggatatcctc gatcgggtgt ggggagcgcc tccgcccctg
     2281 ggagaacgaa aggaaatgga ggagcgcaag gagcggtact accgggatat cctggagaag
     2341 gaattcaaac ccatggacgg ggcggccgac ctggcgcagg ccctcctcga acacggattt
     2401 ctgctggcgg tgggttcttc gggtcccccg gaaaacattg ccctggtggt gcataaactg
     2461 ggccttaccg gtcacatccc cgttcagatt accggccggg acgtgacccg gggaaaaccg
     2521 gatccccagg tgttcctcct ggcctcccgg caattgggag tccgacccgg ggattgtgcc
     2581 gtcatcgagg acgccccggc cggaatcgcc gccgccctgg ccgcaaagat ggtcgccgtc
     2641 ggtttggcgg gcacggtccc catcgagaag ctccggcacg ctcatcgggt ggtcgggtcc
     2701 ctgcgggaac tcacgcctca aagcatcgcc cgcctcatcc gcgaaccccg cgcggggcac
     2761 caatgtccac cctcgacccg atgaaggcgt gagcgggctt cgagaccgca tccctaccgt
     2821 tcgtgggtgg agatccgatc gagtactgtc tcatatcgta ggggtcggac gtggtcttca
     2881 ctctggtacg gacggtccaa tctgatgatc atgtagatca gcaccgccaa aaggaaggcc
     2941 ggcatcgccg tcatgacgat gtgggccgga aagttctccg agccgaagaa ccggcagaac
     3001 gccgggggaa cgataccttc gagaatcacc gaaagccgcc tgaccggggg cagctcttca
     3061 tgggcggcca tgagtcgctc gaaacgatgc cgggcaagct ggtccatgtg tcccgggatt
     3121 tcgctgtaga cggccgtctc gccccgggtc cgggggtcca tgagcttcaa cgacacccac
     3181 accgccccga acgcttcatc cgccgcgccg ctatactcca tgcgcctcat ggcgggatat
     3241 tccttctcgt gcaccgcccg ggcatattcc accagggctt ttcggacctg tgcggcttgc
     3301 tcccggttgg gatggagacg aaggccccgc atgaggttca aggtctgttc ggcttccatc
     3361 ccggcgttca cccggtcgtt gttgtactgg acccacacca cgtagatcac caaggcgagc
     3421 atgactccgc agaccagccc gagagtcgtg aaaatgtacc cggtgacatc gctatggtat
     3481 ttcatcacgt ggtggggtat gaatcgcctg acaacgagga gcccggcaac ggaaaacccc
     3541 acaacgaccc cgatgatcag agacaggctg agccacgggg gaagcatcct gagctttgcc
     3601 ggtaaggaca tgctccgacc tttccctgca gcgggagaat caggactcgg cccctccatg
     3661 aacaggccct tgcctctccc cccggccccc gcggtcacga gagagagacg ggaaccggga
     3721 ctgagcgatt ccgagagcat caacccgtca tgcccgcgac ggcgggcatc cggtggggac
     3781 ccaggctctt tccgaattcc cacctgcgaa agaacgacga aaggggactg gtaccggtca
     3841 gatcgctcaa cccgagggaa aagctccctc tgcccggggc cgcagggtcg atggaagcga
     3901 cctgttccat acggacaagc ttttgcggtt ccacgggcga cgcacggcgc gaagccctct
     3961 actaccatga cggccgccaa gacctgagag gaacgttttc ggtcctttcc accattccag
     4021 aaatcgagag gcaacttccc atgacctcca gaaccttcga aaccctgatg gcggcaaagc
     4081 aaaatctgga gaacatcctg gacaacctga tggagggcct gatcgcccac gacacggaac
     4141 gcaggatcct tttcttcaac cgggccgccg aagccatcac cggctacagc agagacgagg
     4201 tcctgggcag agactgccac caggttttcg ggggtcccct gtgcggcagt cgctgttctt
     4261 tctgcaacgg tcccgtcgag agcctgtccc ggctcgccta ttccttgacc gtccccacca
     4321 agagcggtga gtcccggcgc atcgaaatgt ccgtcacggg aatgttcgat gaaaccgggg
     4381 gcttcatggg ggtcctggcc gccttccggg acgtgaccga cctgatcgga ctgcgtaccc
     4441 gcctggggga agtccaccgc ttctccggga tcatcggccg agatcaccag atgctccagc
     4501 tctacgagca aatccgggag cttgccctga acgattttcc cgtccacatt cgaggagaaa
     4561 cgggaaccgg caaggagctc gtggccaacg ccattcacaa cgagagccac cggggaggcg
     4621 gacccttcgt gcccgtgaac tgcggggcat tgcccgaagg cctcctggaa agcgagctct
     4681 tcggtcacga ccggggggca ttctccggcg ccattcggga caagaagggg cgtttcgaac
     4741 tcgcccacaa ggggaccctc ttcctggacg aggtggccga actccccaag tccatgcagg
     4801 tgaagctcct cagggtcctc cagcagaggg ctttcgaacg ggtaggagga gagaagacca
     4861 tccgggtgga cgttcgaatc atcagcgcca ccaaccggga cctgaagcgg gagatggaga
     4921 ggggcaactt ccgcgaggac ctctattacc ggctcaacgt ggttcccctg tacctgccgt
     4981 ccctgcgcga gcgcaaaaac gatatccccc tgcttgtggc ccacttcctc gaggaagcct
     5041 ccaaccgggg ccaccgggtg gcccgggttt ctcccgaagc catgtcgctt ctcatggacc
     5101 actcgtggcc gggaaacgtg cgcgagcttc agaacgccgt tcatttcgcc ctggtaaaaa
     5161 cccggggctg cgtcatcgaa ccggagcacc ttcccatgga actgcgaaag gagctttgtt
     5221 cgaaatccag gaggggtccg gccccgaagc tcaatccggc ctccgtggaa gcagcccttg
     5281 ccgcttcggg aggaaacaag tccagggcgg caaaggccct caacgtcggc cgtgcaaccc
     5341 tgtatcggtt tctgagccga tccggccctg tctcataata cagaactgtt tcaggttgtt
     5401 tttcgagaca tccgcttccc ggcccgagga gctatctggg ccgggcattc ccaggacgtt
     5461 ctcgtccggg tcgaagaggc cccggggggc ggaatgtatc gcgtatgtct cgggtatgag
     5521 ggtgtccccg gggacggtct ttcccatccg acggcctaag ccgtggaatt tgcttggaag
     5581 accgggcatt caaaccgggc ggttgtccgg catgagactt gccagataag gtccaaagac
     5641 gcaaccggac ccccatggtt cccgccccca ttctcccagg gggacgggaa agccgaggaa
     5701 ggtcctgcgg catgtctcgg tcccccgccc tccaataggg gacctttggg gagcatggag
     5761 acccggtatc cggggcaggt tcggagaaat ctcgacggaa ggtgggaaga tgaggctttg
     5821 tgacgagaac cttttgaaga ccctggaact gacggatcgc atggtggaaa tcgccgatca
     5881 gggagacatg gcccgcgaag acccaggatg cggcgttctc tacggggtac tgagggatgc
     5941 ggctttcaag atcaggaagc tggccgagga cgagaggtcc gtccacatgc gaaagggaaa
     6001 atggcggcag gattgatccc cgtggatcct ggccgcgtgc tgaggaagat ccacgatttc
     6061 ctctctgtag tcgaaaccgg tcaacggacg aaacgttcaa cacgatgcgt aaggagtgaa
     6121 ggatggcgaa caccaagacc caacagaacc tcatggaagc atttgcagga gagtcccagg
     6181 ccaaccggaa gtacctggcc tatgccaaac aggccgaaaa ggaaggcttc acccaggtag
     6241 ccaagctctt ccgagcggcg gccgaggcgg agacggtgca tgcccattcc caccttcgag
     6301 tggcgggggg gatcaagcag acggcggaga atcttcgaga cgccgttgcc ggggagactc
     6361 acgagttcaa gagcatgtat ccggccatga tcgaagcggc caaggaagaa ggaaacaagc
     6421 aggccgagcg gaccttcacc tacgccaacg aggtggaaaa ggtccatgcg gaactctatc
     6481 ggaaagccct cgaaaagatg gaggccatgg aaccggcgga ctattatatt tgctccgtct
     6541 gcggctacac ttgcgaaaac gaggtcccgg aacgctgtcc cgtctgcaac tccaactcga
     6601 aggttttcta ccaggttgac tagaacccgg attgagcgat ttggccggca cgaatcccct
     6661 ttcgtcattg ccgcgcaggc gggaatccat gaagaggtgc gggcccgcag gatgcccgcc
     6721 tttgcgggca tgacggtctg atgggcttgg aatcgctcaa aagatgtgca acgcagtccg
     6781 ggtccccggt ggtcccattt ccgttttaga agaagaggag acctgctttg gctaagatca
     6841 tcgttatcta cgcaacacgc acccagcaga cacgcaagat tgccgaactc atcgccgagg
     6901 ggattcgcct ggagaacgtg gaggcgacgg tggtggacgt gaacgaagcc acgaagatga
     6961 ccgcccggct ctcggaattc gacggcgcgg ttctcggttc ggctacctac cacggcgaaa
     7021 tgctccagcc catgaagacc ttcctgtttg cattggaaaa agccggactg gaaggaaaag
     7081 cgggaggctc tttcggggct ttcgggtgga gtggagaagc ccccgggcgg atctacaaca
     7141 ccctcaagca cgtggtccgg atggacatgg tcaacggtcc gttgatgttg aagtcggcct
     7201 ccctgggggg aggaatcaag atggcccaag attacggaag ggaaatcgcc aagaaggttg
     7261 cggccaaagg ttgaggcgtt ggggaggggg gatgggccgc cgaggatccg gcggacatcc
     7321 ctctcctttc gcggacgggg acgatccccc ggaaagatga gctaatgaat gggggccggc
     7381 ttgcacagat gagcctcctc catggccacc cggccgcatc cccggcagac atatttcatt
     7441 tgggccagtt tgtctttgca tacatgggtg gtatccacct cgggcgtacc acaaaacttg
     7501 caccgactct tgtcgccgca cgggttgcac agatgacccg ggtcatgggc gaccgcaccg
     7561 cacgttttac aggtatacgc cattacgcac cttcctttcc tgcacggacc cggggtagaa
     7621 acgagaactc cggccctccc ggtgcgcccc gtcttggcgg gcccgccggc accggggtcc
     7681 cggagagcaa tctccatgcc ccggctcccg atcttcacgc cctctcaaaa ttctggacga
     7741 actgtccgat ctgaactccc aggtagacgc actgagtccc ttccaggcag gcctcggcat
     7801 ccttgcgttc caggtgagtc ttcagttcgt ggcttccctt gttcaacacc acgatccacg
     7861 cgtccttgtc cggatcaaag cagagggaaa gagccagccg atgttcgtcg atctccggat
     7921 acatttcctt gagcttctgt tccaatacct tggggtcgac actcattgcc ttactccttt
     7981 atgcccatgg aacgactctc catggtcctg cgatgaatat cccgcggacc cgacttcatc
     8041 cgctgcttca gggcaactgg tttctaaata aggactggcc tcggtcaaat caaggcgcgg
     8101 ctacccaagg cgagattgtg ttcccttgaa tccgaacgac ccggttgttt ccttgaaaga
     8161 aaccgagact ggaacgtcat cttgtcgccg gttgctcccc aaacggggac gagccatcgc
     8221 ccccgacccg aagacggccc ggccccgggc cgggtttccc acgaagttgc gctcaggtcg
     8281 gaaccgctca tccacagaat tcagccgctt tctccaaatc ttcactccgc agatccgagc
     8341 gatcttgaac cctacccggt ttcaccggaa tccgcgagca aacggccgac tggggagaag
     8401 cgggaagacc ggcgtgaacc cggtctcggc cggggccgac ggagggaaga tctccgacga
     8461 atctcaaaca ggggcttctt tcgtaggggc gcctgtgaga ctcccgtcat cttttcccgc
     8521 tttttctttt ctcccgtttt cgggaaatgg agtataagag gcatcgaacc ttcttatttc
     8581 attcgggggg atcgattcct tggaaagaca aatcctgaaa ccctgccgtc cggggcagga
     8641 agcgtgcggc agccgcctgc gaggaggacc aggcaacctt cccgtcgggg ttttccctga
     8701 ttgcctccct gatcgaacga tttggcccgc acgtatccgc ctcgtcattt ccacgcaagc
     8761 ggaaatccag aaagagcgca tcttctccgg atgcccgccg ccgcgggcgt gatcgccgga
     8821 tgctctcgga atcgctcgga gcaggtgcct gtctttgccg aggaccggca gcctgtgtcg
     8881 atacgcagcc catgagtatt ttgcgacctc cttctgtgca gtttttctct cgatctcgtg
     8941 cggcggagtg ctgagcatgg ccggaagaaa tgacggaaag gcagaccgtt cgtccccgtt
     9001 tcgccccctg gacttcattc cccacgccgt tgtggtgctg agaggctcca agattctttt
     9061 tgccaatcgg tggacgtacc tggttttcga ttggcagccc gatgaactcg tcggacaacc
     9121 cctgaaagcc ttatgcaggt cggaagaaga ctgccggggt ctgttgcagg agctccgggc
     9181 ctgggacgat tctttggacg tcgtccgtga aggacttccc tgccgacaaa agagcgggaa
     9241 gaccctcatt tgcagggttt cggcctcgtt tcttcccaag gaaggtcgcc aacgcctgca
     9301 gatgctgacc ttcgacgagg tgacgggctc ggccccaccc gattccgcaa cggtccgtct
     9361 ttccctcagc caggcaaaag aagccctgag aaagagggtg cgccaacgca ctctcgagtt
     9421 gcaggaaaca aaccggacac tgaggggcga gattgcggag cggaaaaagg cggagaaggc
     9481 ccttcgcaag tccgagcgac tgctggccga catcatcgag tttctcccgg accccacctt
     9541 cgttgtcgac gccaaacggc agattgttgc gtggaatcgt gccatcgagg acctcaccgg
     9601 ggtacccggc aaacagatgg tcggaaaagg caattacgaa tatgcgatac ccttctacgg
     9661 gacccgaagg cccattctcc tggaccttgt tttcgaaaac cgacatgaaa ttgaagagga
     9721 ataccggatc ctggagcgac gggagagagt cgtgctgggg gaagcctgtt ttaccatgac
     9781 caacggcagg acggtctgcc tttgggggaa agcggctccc atcctggacc atgacgggaa
     9841 ggtgatcggt gccatcgagt gcatccggga cgtcacggag aagaaacagg gggaggaggc
     9901 cctgcgccga tccgaggacc ggttttccaa ggctttcctc agcagtccga ccccgaccct
     9961 cctcacggtc ctgtccgaag acaccatcct ggacgcaaac gaaagcttct gtcgatggtt
    10021 gggctattcg cccacggagc tcatcggccg cacatgccgg gacctacccc tgtgggccaa
    10081 cgcgcaggat caggccgtat tcattgggga tctccgggag aggggctccc tgcggaacga
    10141 atactcccgg gttctcaccc ggaagggaga ggtccgggac atcctggttt ccgcggaaat
    10201 ccttcccacg ggtggggagg ac
//
LOCUS       DAOOVC010000138        10178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4221_length_10178_cov_6.798973, whole genome shotgun sequence.
ACCESSION   DAOOVC010000138 DAOOVC010000000
VERSION     DAOOVC010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4221_length_10178_cov_6.798973"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLM79_14415"
     CDS             <1..678
                     /locus_tag="PLM79_14415"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87553.1"
                     /translation="TIVIDGAHNLKDEAGNALLKILEDPPPRNLFLLLTLESQMVLPT
                     LVSRCCRVQFQPLPDEWIAGYLADTFEIPMDLARALARRSDGSRDRARALAEENQAAR
                     QREVLDRVRRLASPSMMDLFDLTADWAQKTRDLEQDLECVKFWVREILLARIGMNDAS
                     GPDSPEDIGSLARRSTREGLLELYDTVELAMQHLRQNANKLLTLEGVCFAIRRGLYGQ
                     GHWNSLS"
     gene            647..1465
                     /locus_tag="PLM79_14420"
     CDS             647..1465
                     /locus_tag="PLM79_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOV87554.1"
                     /translation="MDRVIGIRFRKGGKVYHFDPAGHPLKKGDYVIVRTEQGVGLGEV
                     VEGPYPKDPRIHPQEIKKVDRSATREEIQTYYQNLEFEQDAKAYCLDRIKAHNLAMNL
                     VNVEYFYDGSKIIFYFTADGRVDFRELLKDLVRRLRTRVELRQIGIRNQAKMVGGLGG
                     CGRPLCCASFLKNFHTVSIKMAKEQNLSLNPTKISGACGRLMCCLQYEYETYKELKKD
                     MPKIGKKIEIPEGRGKVIRQNIVDRTITVQMEDGREIEKKLDPVSEETSEKAKS"
     gene            1462..3375
                     /gene="metG"
                     /locus_tag="PLM79_14425"
     CDS             1462..3375
                     /gene="metG"
                     /locus_tag="PLM79_14425"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOV87555.1"
                     /translation="MTKRFYITTPIYYVNAEPHIGHAYTTIVADVMSRFHRLMGYRTH
                     FLTGTDEHGDKIAQAAAEAGTDPKSYADRISNLFRETWPKLNISNDDFIRTTDPRHIK
                     TVQYILQKVYDQGDIYFSNYKGLYCVGCERFYTERELVDGKCPDHDKKPVEREEANYF
                     FRMSQYQDWLISYIRENPDFIQPERYRNEVLSFLREPLEDLCISRPIQRLSWGIPLPF
                     DDRYVTYVWFDALINYLSALGYPDGDLFREYWPVSRHLIAKDILKPHGIYWPTMIRAL
                     GLPPFRHLNVHGYWKTEEGKMSKSHGTVVRPLDLLPVYGLDAFRYFLLREMVFGLDAG
                     FSEEALVQRLNADLANDLGNLFSRTLAMTAKYFQGKVPPFGSDPDENDRALLAQRDQT
                     TVDFLEEVPRFAFHKALISIWALINAANKYIDQVGPWQLFKDPGKADRLRTVMRNLLE
                     VNRLTAVFLTPFLPDTAEKMLHRLGISKAASDVRFEEDGRWGTLREGAPVDKGEALFP
                     RVDSAQKQGAKRPTVPKKEEKTPDTVPIDTFRTLDLRVGVVKEAHNVPKSQKLLRLIV
                     DIGEERQVVAGIAQWYKPADLVGKQVVLVANLQPAKLMGVESRGMVLAVQDGKALRLI
                     APAEPVAPGSRVS"
     gene            complement(5104..6342)
                     /locus_tag="PLM79_14430"
     CDS             complement(5104..6342)
                     /locus_tag="PLM79_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87556.1"
                     /translation="MLTQTDALALLVENADFGVIGLDARLLIAVWNPWMAARTGVAPE
                     AAQGRPLQKVFPHLPDTSLQVIESVLQTGQTRVLSPSLHEPWGLFPADLRQYVRLQPV
                     RDGRKEIRGLLVIIQDMTGPLEFEEEVERLNHEMTRLLNEIKESNAKLLATTRRLEEV
                     NADMEAFVYSVSHDLRAPLRAIQGFAGVLMESRADQLDSLTLDYLGRMDSAARRMDTL
                     IQDLLSYSRVGRAEMNLMPVYLDSLIAEVLQQMEMDIGVRRAIVDVQGPLPVVMGHYP
                     VLVQVLTNLIGNAVKFVPADVVPRVLIRGEAATEGEAPYPRVRLWIEDNGIGIAPEYQ
                     ERIFHVFERLHGADQYPGTGVGLAIVRKGIARMGGCAGVESVPGQGSRFWIELSAADR
                     TSEERNRLPPEPPMPASRWG"
     gene            complement(6502..7476)
                     /locus_tag="PLM79_14435"
     CDS             complement(6502..7476)
                     /locus_tag="PLM79_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87557.1"
                     /translation="MRILVVDDSPYARRRIVRLLAAAGHEISEADSGASALELFQDEA
                     NRPELVTVDLLMPEMDGMELIRRLRAMDFEVPIVAFSADVQQATRAEAFAAGASEFVS
                     KSEDAERLPDVVQELAETRGGVVLTSVQHDMFTEMMNIAMGQAAMALSQLVKRRVLLK
                     VPTVEIMHASAFVSYLEKAADRVGAAVLQKFSGALNGLGSLVFPEHHAATLVRMLLDT
                     DRQLAQLSSSELTVLTEVGNVVINGALGQLGDVMRIRLKIGLPSLVLNRSATETAGML
                     LSASPNARHAAVLVNCLSIDDVNMIVYLVLMLPKRDLKRLIETLGGSE"
     gene            complement(7630..10041)
                     /locus_tag="PLM79_14440"
     CDS             complement(7630..10041)
                     /locus_tag="PLM79_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87558.1"
                     /translation="MTDLQKALLDWMDGVAATGIFTTDVHLNILTWNQWMAAHSDFDR
                     ARAVGCNLLDLYPDLAHRGLDQFYHSVLAGAPQLISHRLHRYLLPMSPQEATPCRRME
                     QTARIDPLYSAGRVIGTVTVIEDVSQRVAREEELRRQLGMHEVLAEVDLAILTLSLED
                     CLRKVVEGTTRFTGAEICLIALKDEGGLKISACGSIGGSCSTVPDVLDETVVGRVIRS
                     GRPHLSNDLKDSAPGEPVRPVHPDSRSLLAVPLTMDEDVIGVLLLESMRPNAFNEWDR
                     RLMESLAAQTVIAIRNGRMHKALQESEGRYRTVTELSMVGIALMQDGRFRYANPAFAR
                     IFGYSVEEVLNDLRPMDVIAPEDRQLISEQLARRLSGEQEQANYRFRGLHKDGQTIHL
                     EVFSRRVAYEGRPAVLATFLDVTREVQIQAQLNQAQKMEAMGTLAGGIAHDFNNILGV
                     VLGYTELALFEAPKDSALHRNLDQVLKAGRRAKDLVKQILAFSRKGEQERQPLAIGLI
                     VKEALKMLRASLPSTIEIKQHIAVSPGTGGGMVMADPTQIHQVLMNLCTNAAHAMEAK
                     GGILEVSLTDADFEAEPLFRHHGLKPGPYLKLTVSDTGHGMDPATLERIFEPYFTTKA
                     PGEGTGLGLAVVHGIVESHGGKIFVESTPGEGSRFHVILPSIQAAPSAAPEACESIPG
                     GTERILFVDDEPALVKMWQTMLDRLGYQVTAFTSSLEALEAFRAEHDRFDLIVTDQTM
                     PHLTGLDLAGECLRIRSGILVILCTGYSKQVSPETARASGIRELLMKPLVPAQLAQAI
                     RRALG"
BASE COUNT         2152 a         3122 c         2947 g         1957 t
ORIGIN      
        1 accatcgtca ttgacggagc tcacaacctg aaggacgagg ccgggaacgc gctcctcaag
       61 atcctcgaag accctccgcc gcgcaatctc tttcttctgc tcaccctgga atcccagatg
      121 gtcctgccca ccctggtttc ccgctgctgc agggtgcagt tccagccgtt gcccgacgaa
      181 tggatcgccg ggtacctggc ggataccttc gaaatcccca tggatctcgc ccgggccctg
      241 gctcgaaggt ccgacgggag ccgggaccgg gcccgggccc tggccgaaga gaaccaggcg
      301 gcacgacagc gggaagtcct cgaccgggtc cgccgcctgg cgagcccgtc catgatggac
      361 ctattcgacc tgacggcgga ttgggcccag aagacccggg acttggagca agacctcgaa
      421 tgcgttaaat tctgggtgcg ggaaatcctg ctggcccgga taggaatgaa cgacgcttcc
      481 ggcccggact ctcccgaaga catcgggagc cttgcccgcc gctccacccg ggaaggcctg
      541 ctggaattgt acgataccgt ggaactcgcc atgcagcacc ttcgacagaa cgccaacaag
      601 ctactgaccc tcgagggtgt ctgcttcgcc attcggagag gcttatatgg acagggtcat
      661 tggaattcgc tttcgtaaag gaggcaaagt ctaccatttc gatccggcgg ggcatccttt
      721 gaagaaggga gactacgtca tcgtccgcac cgaacaggga gtgggattgg gggaggtggt
      781 ggaaggacct tatcccaagg acccccgcat tcacccccag gaaatcaaga aggtggatcg
      841 ctcggccacc cgggaagaga tccagaccta ttatcagaat ctcgagttcg aacaggacgc
      901 caaggcctac tgcctggatc ggatcaaggc ccacaatctg gccatgaacc tggtcaacgt
      961 cgagtatttc tacgacggga gcaagatcat cttctatttc accgccgacg gccgggtcga
     1021 cttccgggaa ctgctcaagg acctggtgcg ccggttgaga acccgggtcg agctccgcca
     1081 gatcggcatt cgaaaccagg ccaaaatggt gggcggtctc gggggatgcg gtcggcctct
     1141 gtgctgcgcc tccttcctga agaacttcca caccgtgtcc atcaagatgg ccaaggaaca
     1201 gaacctgagc ctgaatccca ccaagatatc cggggcctgc gggcggctca tgtgctgcct
     1261 gcagtacgaa tacgaaactt acaaggaact caaaaaagac atgcccaaaa tcgggaagaa
     1321 gatcgaaatc cccgaagggc gaggaaaggt catccggcag aatatcgtgg accgcaccat
     1381 caccgtgcag atggaagacg gtcgggagat cgagaagaaa ctggatccgg tttccgagga
     1441 gacctcagag aaagccaagt catgacaaag cgcttctata tcaccacccc gatctattac
     1501 gtgaacgccg agccccacat cgggcacgcc tacaccacca tcgtcgccga cgtcatgagc
     1561 cgctttcacc ggctcatggg ctaccggacc catttcctca ccggcaccga cgaacacggc
     1621 gacaagattg cccaggcggc ggccgaggcg ggaacggacc ccaagagcta cgcggaccgg
     1681 atcagcaacc ttttccggga aacctggccc aagctcaaca tctccaacga cgacttcatc
     1741 cgcacgacgg acccccggca catcaagacg gttcagtaca tcctgcagaa ggtctacgac
     1801 cagggagaca tctattttag caattacaaa gggctctact gtgtcggctg cgagcggttt
     1861 tacaccgagc gcgagctggt ggacggcaag tgccccgacc atgacaaaaa gcccgtggaa
     1921 cgggaggaag cgaactactt tttccggatg agtcaatacc aggactggct gatatcctac
     1981 atccgtgaaa accccgattt cattcagccc gaacgataca gaaacgaggt cctgtctttc
     2041 ctccgggaac ccctcgagga cctgtgcatc tcgaggccga tccagcggct cagctggggg
     2101 attccccttc ccttcgacga tcgctacgtc acctacgtgt ggttcgacgc cctcatcaac
     2161 tacctttccg ccctggggta tcccgacggg gacctgttcc gggagtactg gccggtgagc
     2221 cgccacctca tcgccaagga catcctgaaa ccccacggga tctactggcc taccatgatc
     2281 cgcgccctgg ggctcccgcc cttccgccat ctcaacgtcc acgggtactg gaagaccgaa
     2341 gaggggaaga tgagcaagag ccacgggacg gtggtgcggc ccctggatct tctgcccgtc
     2401 tacggactgg acgctttccg ctactttctc ctgcgggaga tggtcttcgg actggatgcg
     2461 ggttttagcg aggaggcctt ggtccagcgg ctcaacgccg accttgccaa cgacctgggc
     2521 aacctcttca gccgaaccct ggccatgacc gccaagtact tccaggggaa ggtgccgccc
     2581 ttcgggtccg acccggacga aaacgaccgc gctctcctgg cccagcggga ccagaccacc
     2641 gtggacttcc tggaggaagt gccccggttt gccttccaca aggccctcat atccatttgg
     2701 gccctcatca acgccgccaa caagtacatc gaccaggtgg gtccctggca gctgttcaag
     2761 gaccccggaa aggcggaccg cctccggacg gtcatgcgaa accttctcga agtgaaccgg
     2821 ctgacggccg tgttccttac ccccttcctc ccggacacgg ccgaaaagat gctccatcgc
     2881 ctgggcatat ccaaggccgc ctcggacgtg cgattcgagg aggacggccg ttgggggacc
     2941 cttcgggagg gcgccccggt ggacaaggga gaagcgctct ttccccgggt cgattcggct
     3001 caaaagcagg gggccaaaag acccacggtc cccaagaaag aagagaaaac cccggacacg
     3061 gttcccatcg acaccttccg caccctggac ctccgggtgg gggtggtcaa ggaggcgcac
     3121 aacgtcccca agtcccaaaa gctcctccga ctcattgtgg acatcgggga agaacgtcag
     3181 gtggtggcgg ggatcgccca atggtacaaa cccgcggacc tggtcggaaa acaggtcgtc
     3241 ctggtggcga accttcagcc ggcaaagctc atgggggtcg aatcccgggg catggtcctg
     3301 gccgtccagg acggcaaggc cctgcggctc atcgcccccg ccgagccggt ggcacccggt
     3361 tcccgggttt cctgaaccgt cttctacggt acttccgaaa atacgccgta atatccatgg
     3421 gatctatggg attccgaacc gcggccggaa ccggcgggtg cgtccggaag accttgcgga
     3481 cggctcgcct cgcgccgtgt tccggaccgg tccacaacgc ctcccaaatc cggcggaaag
     3541 gaaatcgaag caggccaagg gcatgatccg gattccgttc ttgctgaggc agatgctttc
     3601 ctttcggaag agccgacatg aggtcaaccg agcggacgtc cgtttcggaa ctctctgcgc
     3661 gttcctgcgc tcgtcatggg gttatccgca gtgccaagcc ttggttcgtg ggatgactgc
     3721 ccacgcatat cccgaagata ttgaacgaat tctcttgccg atgctcgaaa gcagcgctca
     3781 gcagcgattg gaccaacacg tcattggcga aatggaaggg aaatgggcag ctgtcacgct
     3841 agtcgatgcc gcccgctcag ccgtcgaatc cctcatcgac ggctccctgg acgaagcctc
     3901 tctcctcgcc gagggcgaag ccgtcgagca gcggctcgct gagaatccaa gccctcacaa
     3961 gacggaaaag ccacgaccac acgcaccgca atcgactacc tggacaagct catcgggaat
     4021 gccggcaatc cgtccggcct tcgggtggcc ctgcgcagcc ctttttccgc ccgccgcagc
     4081 gagttcgcgg gcatgccgca caccgagcgg gctggggagc cgggcgactg actgccctgg
     4141 ccgaaggaga ggagatttca ctctggccgg agtcaatccc aacccgaggg caaccatgag
     4201 agagctggat cctgcaatat caaggaacaa cgagagctgc gcgggactcc gacactcctt
     4261 gggacgcaca tattccgccc gctgtggtac aagcggcaac accaacccga ttacaaggaa
     4321 tcgcgaaatg tctttgaact ggcgagagta catcacggtt gatccaaatg tgtgccacgg
     4381 caaggcatgt ataagaggaa cacgcatcct ggtctccgac gtgctcgata gtttggccgc
     4441 cggactgacg agaatgggtc ccccaagact cgtcggaggg ggttcggacg gcggcggcac
     4501 acgacgtttc ttcatgacgg tcttaaagac cacttgccca cagacgccat caaagcgtac
     4561 gggcgagcgt cacgctgttc actaattttc tgaaccgggt gtctcaagat gcttgacaca
     4621 ttccgcggac tgcgactttg agagtctcgc atagttcctg cacaacatca gcaccttcct
     4681 gtcggccgca attcgtgtcc caacgaacgc gagcgggatc ggaccaaggc aacgggacga
     4741 atagattaca caaaacgcaa gaatccagct ttttggggcc ttaagcctgc attttcgtga
     4801 cttaaactgc agctttggcc aaagagtccg agcggtccgg ttccaccttt cgctgacttg
     4861 gttcgtgcgt tagcacctcg tcccggacag gttgcgtcct cgtgacccgt ccaggccggg
     4921 aagaagtcca tggctatgcc tccttaagtt tcttgcagga aatccttaag gcgtatagcc
     4981 cttcttccca atcatttcaa accttttcag tcacttacat gcattctgca aaagttcggt
     5041 ctatgaatat tcggtgggtt ggcgtatccc tttgccagaa aatggtctcg ccattctttc
     5101 tcgtcacccc cacctcgatg cgggcatggg gggctcgggt gggagtcgat tcctctcctc
     5161 cgaggtacgg tccgccgcag acagctcgat ccagaagcgg ctgccctggc ccgggaccga
     5221 ctccacaccg gcacatccac ccatccgggc gattcccttg cgaacaatgg cgagtccgac
     5281 gcctgtgccc ggatattggt cagcgccgtg taatctctca aaaacatgaa atatacgctc
     5341 ctgatactcc ggagcgattc cgatgccgtt gtcttcaatc cagaggcgca cccgcgggta
     5401 aggggcttca ccctccgtcg cggcctcgcc gcggatgagc acacggggga ccacatcggc
     5461 gggcacgaac tttaccgcgt tgccgatcag gttcgtaagt acctggacga gaacgggata
     5521 atggcccatc actaccggca gtgggccttg cacgtcgact atcgcgcgcc ggacgccgat
     5581 atccatctcc atctgctgca gcacttcggc gatcaaggaa tcgagataga ccggcatgag
     5641 gttcatctcc gctcggccga cacggctgta gctcagcagg tcctggatga gagtgtccat
     5701 gcgacgcgca gcgctatcca tgcgtccgag ataatcgagg gtcagggagt ccaattgatc
     5761 cgcgcgggat tccatgagaa cgccggcaaa tccctggatg gccctcagag gggcccgcag
     5821 gtcgtgggag acggaataca cgaacgcttc catgtccgca ttgacctctt ccagtctgcg
     5881 tgtcgtcgcc aggagctttg cattggattc cttgatttca ttcaagagcc gggtcatctc
     5941 gtggttgagc cgctccactt cttcttcaaa ctcgagcggt cccgtcatgt cctgaatgat
     6001 aacgaggagg cccctgatct ctttacgtcc atcgcgtaca ggctgcaacc gcacatattg
     6061 acgcaggtcg gccgggaaaa ggccccaggg ctcatgcagg gacggagaca ggacccgtgt
     6121 ctgaccggtt tggagaacgg attcgatcac ctggagagat gtatccggca gatgaggaaa
     6181 aaccttctgt aaagggcggc cttgagcagc ttccggagcc acgccggtgc gtgccgccat
     6241 ccacgggttc catacggcaa tgagcaggcg agcgtccagg ccgataacgc caaaatcggc
     6301 gttttccacc aggagcgcca gtgcgtcggt ttgtgtaagc aattcagacg cttcgttttt
     6361 caaggaatgc atcgcccatg acctctcttt tcaccgcaaa ggcgcaaaga acgcgaagat
     6421 gccgatctca tcatggaacc ctttgcgctc tttgcgcctt tgcggttaac cacagttctc
     6481 caccgaccgg ggaccggacg ctcattcact tccgccaagg gtttcgatca gccgtttcag
     6541 gtcccgcttg ggcagcatca gcaccagata gacgatcatg ttcacatcat cgatagacag
     6601 gcaattcaca agaacagcgg cgtgacgggc gttgggggaa gcgctgagca gcattccggc
     6661 cgtttccgtg gcggagcggt tgagcacaag ggatgggagc ccgattttga gccttattcg
     6721 catgacatcc ccgagttggc cgagggcgcc gttgatcacc acattgccga cctcggtaag
     6781 gaccgtcagt tcggaggagg agagctgtgc gagctgcctg tcggtatcca ggagcatccg
     6841 caccagagtg gccgcgtggt gctcgggaaa caccagggag cccagaccat tcaaggcccc
     6901 ggaaaacttc tgcaggactg cggcgccgac ccggtccgct gctttttcca ggtagcttac
     6961 aaacgcggaa gcgtgcatga tttcaacggt gggcaccttg agaagcactc gacgcttcac
     7021 caattgactg agcgccatgg cagcctgtcc catggcgata ttcatcattt cggtgaacat
     7081 atcgtgctgc acgctcgtga gcaccacgcc tcccctggtt tcagccagct cttgcaccac
     7141 atcggggagc cgctcggcat cctcgctctt ggagacaaac tcggaggccc ccgccgcgaa
     7201 ggcttcggcc cgtgtggcct gctgcacgtc ggcggagaag gcgacgatgg ggacctcgaa
     7261 gtccatggcc ctcaaccggc ggatcagctc catcccgtcc atctcgggca tgaggaggtc
     7321 gaccgtcacc agctcggggc ggtttgcttc gtcctgaaaa agctccagcg ccgaggcgcc
     7381 gctgtccgct tcgctgattt catggcctgc ggcggccagc agccgtacga tgcgccgccg
     7441 tgcatagggt gaatcgtcta ctacaaggat acgcatgact gcctcctgga atgatattct
     7501 caccacagag gacacagaga gcacagagga aaaaaaacag gaagagcaga aagtcacaat
     7561 tgaaaataca aaagaacctc tctgtactct gtgtcctctg tggtgaatac tggatcgaga
     7621 atccctcctt cagcccaagg cgcgccggat ggcctgggcc agttgtgcgg gaacgagggg
     7681 cttcatgagt aattctcgaa tcccgcttgc cctggccgtt tcggggctca cctgcttgct
     7741 gtaacccgta caaaggataa ccagtatacc ggaccgtatg cgaaggcact ctccggcaag
     7801 gtccagcccc gtcaaatggg gcatggtctg atcggttacg atcaaatcga accggtcgtg
     7861 ctccgctcga aaggcttcca gggcttcaag gctgctcgtg aatgcggtca cctggtagcc
     7921 caggcgatcg agcatcgtct gccacatctt caccagggcc ggttcatcgt ctacaaaaag
     7981 aatgcgctcc gttccaccag gaatgctttc gcaggcttcc ggcgccgcgg agggcgcggc
     8041 ctgaatgctc ggcagaataa catggaagcg gcttccctcg ccgggcgtgc tctccacgaa
     8101 aatcttaccc ccgtgacttt cgacgatgcc gtgcaccacg gccagtccga gtcccgttcc
     8161 ctcaccaggg gccttggtgg tgaaataggg ctcgaagatc cgctccaacg tggccgggtc
     8221 catcccgtgg ccggtatcgc tcaccgtgag cttcaggtaa ggtcccggct tcaagccgtg
     8281 atgccggaat agtggttccg cctcgaagtc cgcatcggtg aggctcacct cgaggatacc
     8341 gcccttcgct tccatggcat gggctgcgtt ggtgcacagg ttcatcagga cctgatggat
     8401 ctgggtcgga tcggccatca ccatccctcc gccggtcccc ggcgaaacgg cgatgtgctg
     8461 tttgatctcg atggtggaag gcagggaggc gcgcagcatt ttaagggctt ccttgacgat
     8521 caacccgatg gccagaggtt ggcgctcctg ctcccctttg cggctgaagg ccaggatctg
     8581 cttcaccagg tccttggctc ggcggcccgc cttcaacacc tggtccaggt tccggtgaag
     8641 agcggaatcc tttggagctt caaaaagggc caattccgtg tagcccagca ccacccccag
     8701 gatattgttg aagtcatggg cgatgcctcc cgcgagggtc cccatggctt ccatcttctg
     8761 ggcctggttt agctgcgcct gtatctgtac ttcgcgggtg acatccagga acgtcgcaag
     8821 caccgcggga cgaccttcat aagccacccg ccggctgaac acttcgaggt ggattgtttg
     8881 accgtccttg tggagaccgc ggaaacggta gtttgcctgc tcctgctcac cggaaagacg
     8941 ccgcgcgagt tgctcggaga tcaactgacg atcttccggg gcgataacgt ccatgggcct
     9001 caggtcgtta aggacctcct cgaccgagta gccgaagatg cgggcaaaag ccggattggc
     9061 ataccggaag cggccatcct gcattagagc gatccctacc atggacagct ccgtaacggt
     9121 gcgatatcgt ccttcggatt cctggagcgc tttatgcatc cgcccgttgc ggatggcgat
     9181 gacagtctgg gccgccaggg attccatcaa gcgccgatcc cattcgttga atgcgttggg
     9241 gcgcatggac tcgagcagga gtactccgat gacgtcctca tccatggtca gcgggaccgc
     9301 aagaagcgac cggctgtcgg ggtgcacggg acgaacgggc tcacccggcg ccgaatcctt
     9361 caagtcattg gataggtgcg gccggccgga ccgaatcacc cggcctacga cggtttcgtc
     9421 gaggacatcc ggcactgtgg agcatgagcc gccaatgctg ccgcatgcgg agatcttcaa
     9481 tccgccttca tccttcaggg cgatgaggca gatttcagcc ccggtaaagc gcgtcgtccc
     9541 ctccaccacc ttccgcagac agtcctcgag acttagagtc aaaatggcga ggtccacctc
     9601 ggcgagcact tcatgcatgc ccagttgtcg ccggagctct tcctcacggg ccacccgctg
     9661 ggaaacgtcc tcgatgaccg ttacggtccc gatgacacgg cctgcggaat agaggggatc
     9721 gatacgggcg gtctgctcca tgcgccggca gggagtggct tcctgggggc tcatgggcag
     9781 caggtaccgg tgcagcctgt gggagataag ctgcggcgca ccggccagaa cagagtgata
     9841 gaattgatcc aggccgcggt gcgccagatc ggggtagaga tcgagcaggt tgcaccccac
     9901 ggcccgggcc cggtcgaagt cgctgtgggc tgccatccat tggttccagg tcaagatgtt
     9961 cagatggacg tcggtggtga aaatacccgt ggcggcaaca ccgtccatcc agtcgagcag
    10021 tgctttctgt aaatcggtca tgtgtctcgc tgctcccagc agttatattt ttcaccacgg
    10081 agagcacaga ggacacagag ataaattatt tgtttatttc tctactcaag tttcgcacat
    10141 gttttaagtg ctttccaaca tagcacgatc aattaaat
//
LOCUS       DAOOVC010000139        10073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4259_length_10073_cov_7.508185, whole genome shotgun sequence.
ACCESSION   DAOOVC010000139 DAOOVC010000000
VERSION     DAOOVC010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10073
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_10073_cov_7.508185"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /gene="rimI"
                     /locus_tag="PLM79_14445"
     CDS             <1..532
                     /gene="rimI"
                     /locus_tag="PLM79_14445"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOV87559.1"
                     /translation="PQRSSADSMAIYLEPMTSQDVESILEIESESNLEPWSARCFLEE
                     MGRSYSRVRVARDGHRMGRIVGYICFWLIVDELQILNVAVHKDYRRRGIGRKLLSYAL
                     DTGVVRNARVAVLEVRQSNTSARSLYGQLGFRPLGIRPNYYGGITESAILMELDLKDY
                     RTSEAPRETLGLCVEG"
     gene            537..4808
                     /locus_tag="PLM79_14450"
     CDS             537..4808
                     /locus_tag="PLM79_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOV87560.1"
                     /translation="MNQVLPVDHIQSDALNRNMDLTNVEVVIPEVFEVLREAVKGYAG
                     KEQQAHELLREFHHRFRNWEFVIHETWRYAVSNMRVFRQHPRNGRILALLSQILLEAL
                     IQSESAANRNLAADHLIALWIKVVEEMPAAVAASAPEEEGVDGEETTLETITTMHQGV
                     LRTYLDRLHHLPQNLFEHVMGSYYPLKRLAGKLFRLWHDPSSFPELRRLMERLLVDSY
                     RFWLQRQDPAEWLARQSGGSPEAAPWFDLVSPLSHHRLRSLLSRMEEEIASQEDDRAA
                     VGKMLEFPDYHDIVRAYHALPEKLKAFDDSASGVHPSMLMRLRIMETKGLEGIHEEAL
                     REINFEIEKWVRQEPVDKVRDLLDRVFEVLGGCMRKHPQAALQIVRTVAMEIMDTNDR
                     PLIEYFLHRVIQMGFQTPELGGVNQHWQMDVNPAHLLNVRIWQDIIRKNPSRTRNLLS
                     ALIVNLTLGGIYVRDTDLFQKDVSELLHAPIQPVYNLVKQLTKLFPVYFNQIGAEGQL
                     RAVSTDIDEITGRTDRLIHFLRKQSHVESNNVIVGFIEAIMTFWKTLDKDRIRDHVPP
                     EVFEDVATSGPLVEEVRRIFESIFAAPNIHHASDLLDLTERQIRDLIWAVPDVSERER
                     QRAFLLIQLYQLLHEKYALSFKDIQVHLRKAATIGLPNPGDLVEALDHPDPIRQLEAT
                     LDYLVALKKVILEPSELQITENIYYKRHIAVDIPSMYGSYNERKFDALGLTFRLESLA
                     NLLFEEIIYSFNLSFITHATFFRIARVLPLFIRALEIDGITSNRLARQEELFQRAIEI
                     RRFSHSQYMDIFRGFSEAIKQIIQTNYHAAHEENLELILKQLNNDRLLPRYRRDGHGE
                     TPAERAQRLSESFLRDLIAGTFGLQYFDHFISSILTTLATQKEELSADLLDLLLSYDP
                     EKTLSTVYDPNPKCYDLIHMGNKGYTLAMLHSMGFRVPPAFVITTEYFRCRSVIESLN
                     PPSEDFEQRVMDHIGKLEQEMGRKFACPQNPLLLSVRSGSAVSMPGMMNTFLNVGINE
                     KIVEGLIAQTGEGWFAWDNYRRFLQSWGMSFDIQRNEFDAIMRHYKARYRRQVKREFL
                     PEEIRELALAYKKAILDRGIDFSDDPKEQLFTAIRQVMASWHVPKAVTYREIMGLSEN
                     WGTAVTIQAMVYGNLDTRSGAGVMFTHDPWTSEDKVWVVGDFTLGNQGEDVVGGLVKT
                     LPLSEAQRLSQGERKEHSLETLFPRVYQHLVKVAKDLIYGQNWAPQEIEFTFQGDHED
                     NVFILQSRDMSPRVRRRYPVFRSTESLHNEYVGSGIGVSGGALSGLVAFDLESISQLR
                     REHADKAVILLRSDTVPDDIREISIADGILTGKGGATSHAAIVAHRLGKTCVVGFSKM
                     RLWEVDKKCTIDGHVIHTGDTIGIDGRSGAVYIGAHEIEHVEIVN"
     gene            4874..6208
                     /locus_tag="PLM79_14455"
     CDS             4874..6208
                     /locus_tag="PLM79_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOV87561.1"
                     /translation="MTGEEKLVPMKLGINGLGRIAKLSIWHHVERQYFSELVVNIGRE
                     VGTRLEDIASFIEKDSTYGALHNYLYGSRARRVVEEINDREGTMRINGVKATFLRKSR
                     NPMAIGWRDHDVNLVVDATGAFLDPTLASDETKGSLRGHLAAGAKKVILSAPFKIRDK
                     SREMPADAVTTVMGINDADYDPKKHLLISNASCTTTCLAYMVKPLMDYFGHRRILSAS
                     MTTVHAATSSQQVLDRAPKAKATDLRKTRSILNNIILTTTGAARALGLVIPEMKRIGF
                     IAESVRVPTSSGSLIILVVMIQDESMRNPINREKINQIYRDAVSHYPEGYLVYSEEQN
                     VSSDIIGLPRAAAVIEGHENHTRTAAVRIDMERVLDACNYLNSLGEASNPSSLDVTQI
                     HVEGRSPRNPGQEEKVLEVPVTQAVIYGWYDNELGSYTNMLGDRTVSIARQM"
     gene            6349..7359
                     /gene="gap"
                     /locus_tag="PLM79_14460"
     CDS             6349..7359
                     /gene="gap"
                     /locus_tag="PLM79_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOV87562.1"
                     /translation="MATRVAINGFGRIGRTIFKALLDRKIPLEVVAINDLETPVELAY
                     LLKYDSVHGVWPHEVSGTENELIVDGKSFQCVKISDPAQSPWRDLKVAIVLESSGRFA
                     DRDSCQKHINAGAQKVIVTAPGKGMDATFVIGVNEGTYDPRIHHIVSNASCTTNCLAP
                     IVSLLHDRFVVEHGLMTTIHSYTMDQRLLDALHKDRRRSRAAALSMIPTTTGAAKAVA
                     EVIPEMKGRLDGFAIRVPTPNVSLVDFVCRVGRDVTKEEVNQVMEEAANGPLKGVLQY
                     VKESLVSVDFNHTPFSSNVDAQLTNVIGKRMVKVCSWYDNEYGYSSRVTELAAMMGEM
                     LE"
     gene            7405..8604
                     /locus_tag="PLM79_14465"
     CDS             7405..8604
                     /locus_tag="PLM79_14465"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOV87563.1"
                     /translation="MRTLDDIDVAGKRVFVRVDFNVPLDKNRRVKDDLRIRAVLPTIR
                     KVLDSKGKLILASHLGRPKGKPAEEFSLKPVGEYLARLLDLPVVPASDCVGREVERQA
                     AGMEPGQILLLENLRFHPEEEKNDPEFSRRLASLAEVYVNDAFAVSHRAHASVHGITR
                     FVPVCAAGYQLEKEVKYFREAMEDPKRPVAMIIGGAKVSTKIELLENLVAKADFLLIA
                     GAMANTFLKAQGKEVGKSLVEDDHLETAARLLKKAQERGVKVYLPVDAVAAPALEVCG
                     SAQQVSVDRVPSDLLMLDVGSGTLEVFESVLRTCRTIVWNGPLGAFETTPFHQGTFAM
                     AKFLAGLDALTVVGGGDSAAAVRQAGLEDRMGYVSTGGGAFLEMLEGKTLPGLAALEE
                     CRQRTQP"
     gene            8616..9386
                     /gene="tpiA"
                     /locus_tag="PLM79_14470"
     CDS             8616..9386
                     /gene="tpiA"
                     /locus_tag="PLM79_14470"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOV87564.1"
                     /translation="MKNRRSLVAANWKMHKTVGETVSFLEALQDRIEAYEDREVVVAP
                     PFTALAAARQAMEGNKINLSAQNCHWEEKGAFTGEVSPAMLKDLGCDYVIVGHSERRH
                     LFGETDEIVQKKTASVFAHGMVPILCVGEVLDEREAGRTFPVVNHQVERALDGLNCRL
                     VPALVLAYEPVWAIGTGKTATPDQAQEVHSFLREKLSALFDKTIANKIRILYGGSVKP
                     DNVDALMAQPDVDGLLVGGAGLEVDSFQRIVQYRFPGE"
     gene            9395..9904
                     /gene="secG"
                     /locus_tag="PLM79_14475"
     CDS             9395..9904
                     /gene="secG"
                     /locus_tag="PLM79_14475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOV87565.1"
                     /translation="MYTFTVFIHISACVALILIVLLQTGRGAEIGAAFGGASNTLFGG
                     SGGSTFLSKLTTAAAIVFMLTCLGLTLLSSAPKTKSIMENVRVEVPQGPALPEAKPVA
                     PSAEQPQPAQSVPATGSEAAVPQSTPAQPAEPAAVEQPAKQEETTPPAEKAPAQKPDK
                     APAKGAKKP"
     gene            9988..10073
                     /locus_tag="PLM79_14480"
     tRNA            9988..10073
                     /locus_tag="PLM79_14480"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10023..10025,aa:Leu,seq:gag)
BASE COUNT         2196 a         2986 c         3017 g         1874 t
ORIGIN      
        1 ccctcaaagg agctcagcgg actccatggc gatctacctg gagccgatga cctcccagga
       61 cgtggagtcc atcctcgaaa tcgaatcgga aagcaatctc gagccgtggt ccgcccggtg
      121 tttcttggaa gagatgggac ggagctactc ccgggtgagg gtcgctcgag acgggcaccg
      181 catgggaaga atcgtcggct atatctgctt ctggctcatc gtggacgagc tccagatcct
      241 gaacgtggcg gtgcataagg attatcgccg caggggaatc ggcaggaagc tcctgtctta
      301 cgccctggat acgggtgtgg tccggaacgc tcgagtggcc gtactggaag tgcggcagag
      361 caatacttcc gcccggagcc tctatgggca attgggcttc aggcccctgg gcatacgccc
      421 gaattactac ggaggaatta cggaatcggc cattctcatg gaactcgacc tcaaggatta
      481 tcgaacgtcg gaagctcccc gggagaccct tggactttgc gtggaaggct aactacatga
      541 accaagtcct gccagtcgat cacattcaat cggacgctct gaatcgcaac atggatctga
      601 ccaacgtcga ggtggtcatt cccgaagtat ttgaagtcct tcgagaagcg gtgaaggggt
      661 acgcaggaaa ggagcagcag gctcacgagt tgctgcggga atttcaccac cgtttccgaa
      721 actgggaatt cgtcattcat gaaacctggc gttacgcggt ctcgaacatg cgcgtgttcc
      781 ggcagcaccc tcgcaacggg cgcatcctcg ccctcctgtc ccagattctt ctggaggccc
      841 tcattcaatc ggagagcgcg gccaaccgga acctggcggc ggatcacctc atcgccctct
      901 ggatcaaggt agtggaagag atgcccgcgg ccgtggcggc ctccgccccg gaggaggaag
      961 gcgtcgacgg ggaggaaacg accctcgaga ccatcaccac catgcatcag ggggtcctga
     1021 ggacctacct ggacaggctc caccaccttc cccagaacct cttcgaacat gtcatgggca
     1081 gctattaccc attgaagcgc ctggcgggaa agctcttccg gttgtggcat gatccgtcct
     1141 ccttccccga actccgcagg ctcatggaac gtctcctcgt cgacagctac cggttctggc
     1201 tccagcgaca ggacccggcc gagtggcttg cccggcaatc ggggggctcc ccggaggcgg
     1261 ccccctggtt tgacctggtg tcgcccttga gtcaccatcg attgagatcc ctcttgagcc
     1321 gcatggagga ggaaatcgcc tcccaggaag acgaccgggc ggccgtgggg aaaatgctcg
     1381 agtttcccga ctaccacgat atcgtgcgcg cctatcacgc actcccggag aagctcaaag
     1441 ccttcgacga ttccgcatcg ggcgttcatc ccagcatgct catgcggctg aggatcatgg
     1501 aaaccaaggg cctggaaggg atccacgagg aggccctgcg cgagatcaac ttcgaaatcg
     1561 aaaagtgggt ccgccaggaa cccgtggata aggttcggga tttgctcgat cgggttttcg
     1621 aagtgctggg cggctgcatg cgcaaacacc cccaggccgc cctccagatc gttcgaacgg
     1681 tggccatgga aatcatggat accaacgatc ggccgctcat cgagtacttc ctccaccggg
     1741 tcatccaaat ggggttccag accccggaac tggggggagt gaatcagcac tggcaaatgg
     1801 atgtgaaccc cgcccatctg ctcaacgtgc gcatctggca ggatatcatc cggaagaatc
     1861 cctcgaggac ccggaatctc ttgtcggccc tcattgtgaa cctcaccctg ggcggcattt
     1921 atgtccggga cacggacctg tttcagaagg acgtgagcga gctgcttcac gcacccattc
     1981 agcccgtcta caacctggta aagcaattga ccaaactttt cccggtgtac ttcaaccaga
     2041 tcggcgccga agggcagttg agggccgtat ccacggatat tgacgaaatc accggaagaa
     2101 cggaccggtt gatccatttt ttgcgaaaac agagccacgt ggaaagcaac aacgtcatcg
     2161 tgggcttcat cgaggccatc atgacgttct ggaagaccct ggacaaggat cgtatccggg
     2221 atcacgtccc gccggaggtt ttcgaagacg tggccacttc gggccccttg gtggaggaag
     2281 tccgacgaat tttcgaatcg atctttgccg cacccaacat ccaccacgcc tcggacctgc
     2341 tggacctcac cgaacggcag atccgggact tgatctgggc cgtgcccgac gtctccgagc
     2401 gggaacggca gagagctttc ctgctcatcc agctctacca gctcctccac gaaaaatacg
     2461 cgttgagctt caaagacatc caggtccacc tccggaaagc cgcgaccatt ggcctcccca
     2521 atcccggaga tctcgtggag gccctcgatc acccggatcc catccggcag ctcgaggcca
     2581 ccctcgacta cctggtggcc ctcaagaagg tcattctcga gccctcggag ctgcagatca
     2641 cggaaaacat ctactacaag cgacacatcg ccgtggacat tccgtccatg tacggatctt
     2701 acaacgagcg caagttcgat gccctggggt tgaccttccg gctcgagagc ctggccaatc
     2761 tccttttcga ggagattatc tattccttca acctcagttt catcacccat gccacctttt
     2821 tccgcattgc ccgagttctg cccctgttca tccgggccct cgagatcgac ggaatcacgt
     2881 ccaaccggtt ggctcgccag gaggaactct tccagagagc catcgaaatc cggcgctttt
     2941 ctcactccca gtacatggat atcttccggg ggttttccga agccatcaag cagatcattc
     3001 aaaccaacta tcatgcggcc cacgaagaaa acctggaatt gatcctcaaa caactaaaca
     3061 acgaccggct gctgcctcga tatcgccggg acgggcacgg ggagacccct gcagagaggg
     3121 cccagcgcct gtcggaaagc ttcttgcggg acttgatcgc cggaaccttc ggcctccagt
     3181 acttcgatca cttcatcagc agcatcctga ccaccctggc gacccaaaag gaggagctga
     3241 gcgccgatct gctggacctt ctgctgagtt atgatcccga aaagaccctc tccaccgtgt
     3301 acgaccccaa tcccaagtgt tacgacctca ttcacatggg aaacaagggc tatacccttg
     3361 ccatgctcca ctcgatgggg tttcgagtcc cccccgcgtt cgtcatcacc accgagtact
     3421 tccgctgccg aagcgtcatc gagagtctga atccgcccag cgaagatttc gaacagcggg
     3481 tcatggatca catcgggaag ctggaacagg agatgggaag gaaattcgcc tgtccgcaaa
     3541 accccctgct cctgtccgtt cgcagcggat cggcggtttc catgccggga atgatgaata
     3601 ccttcctgaa tgtgggaatc aatgagaaga tcgtggaggg actcatcgcc cagaccgggg
     3661 aaggatggtt tgcatgggac aactaccgcc gctttctcca gtcatgggga atgtccttcg
     3721 acatccagag gaacgaattc gatgccatca tgcggcatta caaggcacgg taccgcaggc
     3781 aggtgaagcg ggaatttctg cccgaggaga ttcgcgagct ggccttggcc tacaagaagg
     3841 ccatcctgga ccggggcatc gacttttccg acgaccccaa ggaacagctc ttcacggcca
     3901 tccggcaggt catggcatcc tggcacgtgc ccaaagccgt cacctaccgg gagatcatgg
     3961 ggctgtcgga aaactgggga accgccgtga ccatccaggc catggtgtac ggaaacctgg
     4021 atacccgatc gggggccgga gtgatgttca cccatgaccc gtggacctcc gaagacaagg
     4081 tgtgggtcgt gggggatttc accctgggaa accaggggga ggacgtcgtg ggagggttgg
     4141 tgaaaaccct gcccctgtcc gaagcccaac ggctgagcca gggagaacgc aaagagcact
     4201 ctctcgagac cctctttccg cgagtctacc agcacctggt caaggtcgcc aaggacctca
     4261 tctacggaca gaattgggcg ccccaggaaa tcgagttcac cttccaggga gatcacgagg
     4321 acaacgtctt tatcttgcag tcccgcgaca tgtctccccg ggttcggagg cgctacccgg
     4381 tctttcgaag cacggaaagc ctccacaacg agtacgtggg cagcggcatc ggcgtcagcg
     4441 ggggggcact gtccggcctg gtggctttcg acctggaatc catctcgcag ctgcgcaggg
     4501 agcatgcgga caaggccgtc attctccttc ggtcggacac cgtgcccgac gacatccggg
     4561 agatcagcat cgccgacggg attctcacgg gcaaaggggg agctacctcc catgcggcca
     4621 ttgtggcgca tcgactggga aagacctgcg tggtgggctt tagcaagatg cgcctctggg
     4681 aggtggacaa gaaatgcacc attgacggac atgttatcca tacgggagat accatcggaa
     4741 ttgatggacg cagtggggcc gtgtatatcg gtgcccatga gatcgagcat gtggaaatcg
     4801 tgaattagca gactcgcgcc gtcccatgcc gggtcggatt ttcccaattg ttgggggaca
     4861 ggggggagcg gacatgacgg gagaagaaaa actggtgccg atgaagctgg ggatcaacgg
     4921 tttgggaaga attgccaagt tgagcatttg gcaccatgtg gaacgacagt atttctccga
     4981 gctggtggtg aatatcggac gagaagtggg aacgcggctc gaggatattg cgagcttcat
     5041 cgagaaggat tccacttacg gtgcgttgca caactatctg tacggttccc gggcacggag
     5101 ggtcgtcgag gaaatcaacg accgggaagg aaccatgcgc atcaacggcg tgaaagccac
     5161 gtttcttcga aagagccgga atcccatggc catcggctgg cgggatcacg atgtgaacct
     5221 ggtggtggat gcaacgggtg ccttcctgga tcccactctt gcttcggacg aaaccaaggg
     5281 atccctgcgg ggacacctgg ctgcgggtgc caagaaggtc attctttcgg ctcccttcaa
     5341 gatcagggac aagagccgcg agatgcccgc cgatgccgtg accaccgtca tggggatcaa
     5401 cgacgccgac tacgatccca agaagcacct gttgatttcc aacgcatcct gcaccaccac
     5461 atgcctggcc tatatggtga aacccctcat ggattatttc ggacaccggc ggatcctctc
     5521 ggcgtccatg accacggtcc atgcggccac cagctcgcag caggtgctgg atcgagcccc
     5581 caaggccaag gcgacggact tgcgaaagac ccggtccatt ctcaacaaca tcatcctcac
     5641 caccaccgga gcggctcgcg ccctggggtt ggtcattccg gaaatgaagc gaatcggctt
     5701 catcgccgaa tcggttcggg tgcccaccag ctccgggtcc ctcatcatcc tggtggtgat
     5761 gatccaggac gaaagcatga gaaaccccat caaccgggaa aagatcaacc agatttacag
     5821 agacgccgtc agccactatc ccgaaggcta tctggtgtat tccgaggaac agaacgtctc
     5881 ttcggacatc atcgggctgc cgagggcggc ggcggtcatc gagggccacg agaatcacac
     5941 ccggaccgca gcggtccgca ttgatatgga gcgggtcctg gatgcctgca attacttgaa
     6001 ctccttggga gaggcctcca accccagctc cctggacgtg actcagatcc atgtcgaagg
     6061 acgaagcccc cgcaatccag gccaagagga aaaggtcctc gaggttcccg tgactcaggc
     6121 cgtcatttac ggttggtacg acaacgagct gggaagctac accaacatgc tcggggaccg
     6181 aaccgtgagc atcgcgcgtc agatgtagat ccggagggat tcgctcccat gggtgccgaa
     6241 tcctttgaaa gaatggatcc accggcaaac cttccccatc gaggtttgcc gtgaaacggg
     6301 tatggaggaa agaagccgat tccggcgcaa cgtaaaggag gaagttctat ggctactcgt
     6361 gtggcaatca acggttttgg aagaatcggc cgcaccattt tcaaagcgct cctcgatcga
     6421 aagatccccc tggaggtcgt cgccatcaat gacctggaga cgccggtgga gctggcgtac
     6481 cttttgaaat acgactcggt gcacggcgtt tggccccatg aggtttcagg gacggaaaac
     6541 gagttgatcg tggacggcaa gtccttccaa tgcgtcaaga tttccgatcc cgcccaatcc
     6601 ccctggcgtg acctcaaagt ggccatcgtc ctggagagca gcggccgttt cgcggaccgg
     6661 gacagctgtc agaagcatat caacgcaggg gctcaaaagg tcatcgtcac cgcgccggga
     6721 aaaggaatgg acgccacgtt cgtcatcggg gtcaacgagg ggacctacga cccccgcatt
     6781 catcatatcg tctccaacgc gtcctgcacc accaactgcc tggcccccat tgtgtccctg
     6841 ctccatgacc ggttcgtggt cgagcacggg ctcatgacca ccatccactc ctacaccatg
     6901 gaccagagac tcctggatgc tctgcacaaa gaccgcagaa ggtcccgggc ggcggccctt
     6961 tccatgattc ccaccaccac aggtgccgcc aaggccgtgg ccgaagtgat cccggaaatg
     7021 aagggcaggc tggacggttt tgccattcgc gtcccgactc ccaatgtctc cctggtggac
     7081 ttcgtgtgcc gggtggggcg ggacgtcacc aaggaagagg tgaaccaggt gatggaagag
     7141 gcggccaacg gtcccctcaa aggggtcctt cagtatgtca aggaatccct ggtgtccgtg
     7201 gacttcaacc ataccccctt ctcttccaac gtggacgctc agctcaccaa cgtgatcggc
     7261 aaacgaatgg tcaaagtgtg cagctggtac gacaacgagt acggatattc ctcgcgggtg
     7321 accgaattgg ccgccatgat gggcgaaatg ctggaatagg aaatggatgg gattccgacg
     7381 gtagcgccga gaggaggagg cctcatgaga accctggacg acatcgatgt ggcggggaaa
     7441 agggtgtttg tccgcgtgga cttcaatgtt cccctggaca agaaccgccg ggtgaaagac
     7501 gacctgagga ttcgagccgt cttgccgacg atccgcaagg tcctcgatag caaggggaag
     7561 ctcatcctgg cttcccacct gggcaggccc aagggaaaac ccgccgagga gttttccctg
     7621 aagcccgtgg gcgaatacct ggcccgactg ctcgatctgc ccgtcgtccc ggcatcggac
     7681 tgcgtgggcc gggaggtcga gaggcaggca gccgggatgg aaccggggca gatcctgttg
     7741 ctcgaaaacc tgcgctttca ccccgaagag gaaaagaacg acccggagtt ttcccgccgg
     7801 ctggcgtccc tggccgaggt gtatgtgaac gacgccttcg ccgtttccca tcgagcccat
     7861 gcgtcggtcc acgggatcac ccggtttgtg ccggtctgcg ctgcgggcta ccagttggaa
     7921 aaagaggtga agtacttccg ggaggccatg gaagacccca agaggccggt agccatgatc
     7981 atcggggggg ccaaggtctc caccaagatc gaactgctgg aaaatctcgt tgccaaggcg
     8041 gatttcctcc tcattgccgg ggccatggcc aacacctttc tcaaggccca ggggaaggaa
     8101 gtgggaaaat ccctggtgga ggacgatcac ctggagaccg cggctcggct cttgaaaaaa
     8161 gcccaggaac ggggagtcaa ggtctacctg ccggtggatg cggtggcggc cccggccctc
     8221 gaggtgtgcg gatcagccca acaggtttcc gtggatcgag tcccctccga cctgctgatg
     8281 ctggatgtcg gctcggggac cctcgaggtc tttgaatcgg tccttcgaac ctgccgcacc
     8341 atcgtttgga acggtcccct aggggccttc gaaaccactc ccttccacca ggggaccttt
     8401 gccatggcga aatttctcgc agggctggac gccctcacgg tggtaggggg aggagattcg
     8461 gcggcggccg tgcgacaggc gggactcgaa gatcgcatgg gctacgtttc cacgggaggg
     8521 ggagctttcc tggaaatgct cgaggggaaa accctgccgg gcttggccgc gctcgaagaa
     8581 tgccggcaac gaacgcaacc ctaggagatg aatccatgaa gaatcggcgg tcgctcgtgg
     8641 cggccaactg gaagatgcac aagacggtgg gggagacggt ctcgtttctc gaagccctgc
     8701 aggatcgcat cgaggcctac gaagaccggg aagtggtggt ggcccctccc ttcacggccc
     8761 tggcggccgc aagacaggcc atggaaggca ataagatcaa cctttcggcc cagaattgcc
     8821 attgggaaga gaagggcgcc ttcaccggag aggtatcccc ggccatgctc aaggatctgg
     8881 gctgcgatta cgtgatcgtg gggcactcgg agagaagaca tcttttcggg gaaacggacg
     8941 agatcgtaca aaaaaagacg gcgtccgtgt tcgcccacgg catggtgccc atcctgtgcg
     9001 tgggggaagt gctcgatgag cgcgaggcgg ggagaacctt cccggtggtg aaccatcagg
     9061 tggagcgcgc cctggacgga ttgaactgcc gcctcgttcc ggccctcgtc ctggcctacg
     9121 agcccgtgtg ggccattgga acgggcaaga cggcaacgcc ggaccaggcc caggaggtgc
     9181 actcgttcct ccgggagaag ctatccgccc ttttcgacaa aaccattgca aacaaaatca
     9241 gaatattgta tggaggctcc gttaaaccgg acaacgtgga tgccctcatg gcgcagcccg
     9301 acgtggacgg cctcctggtg ggcggcgccg gcctcgaggt cgactccttt cagcgcattg
     9361 tccagtatcg gttccccgga gagtagaaaa cgagatgtat acgtttacgg tattcattca
     9421 tatctcagcg tgcgtggccc tgattctcat cgtgttgctc cagacgggcc ggggcgcgga
     9481 gattggagcg gctttcggag gagcttccaa cacgcttttt ggcggatcgg gaggctccac
     9541 gtttctgagc aagctcacca cggcggcggc cattgtcttc atgctgacct gcctggggct
     9601 gacgctcctg tcctccgctc ccaagaccaa gtccatcatg gaaaacgtgc gggtggaggt
     9661 accccagggg cctgcactac ccgaggcgaa gccggtggcc ccgtcggcgg aacagcccca
     9721 gcctgcgcaa tccgtcccgg caaccgggtc cgaggcggcg gtgccgcagt caaccccggc
     9781 tcaaccggcg gaacccgcag ccgtggagca accggccaag caggaagaaa cgactcctcc
     9841 ggccgagaag gccccggccc agaagcccga caaggctcct gcaaaggggg ccaagaagcc
     9901 gtagcatcgc gtccgtgaac ggtcgacccg ggacaggggc cggtttgggc ttgccaaagc
     9961 cgaccgttgg gatagaatag tctttgtgcc gaagtggtgg aatctggtag acacaccatc
    10021 ttgagggggt ggcggggcga cccgtgccgg ttcgagtccg gccttcggca cca
//
LOCUS       DAOOVC010000140        10060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4263_length_10060_cov_6.332634, whole genome shotgun sequence.
ACCESSION   DAOOVC010000140 DAOOVC010000000
VERSION     DAOOVC010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_10060_cov_6.332634"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLM79_14485"
     CDS             complement(<1..793)
                     /locus_tag="PLM79_14485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOV87566.1"
                     /translation="MKSKDMVRAGWLMDGSSGSIRRDVFLEMENGFVTELGWNGSRGH
                     DPYEDKSHRRVWDLSDCTLIPGLIDCHVHLSMSGTPDRGEREHQLSRTFEEVKPVIEE
                     HLRHHASRGIVAVRDGGDAHGHTLWYKKRWMKEDCVPVRLKSPGKAWRSPGRYGRLIG
                     DPPPEGSSLADCVARYARSADHVKIVNSGLNSLREFGKETLPQFNLDELRMAVRRGEE
                     LGRKTMVHANGKGPVSMALKAGCHSIEHGFFMQKDNLERLAEQQVY"
     gene            complement(881..1318)
                     /locus_tag="PLM79_14490"
     CDS             complement(881..1318)
                     /locus_tag="PLM79_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87567.1"
                     /translation="MILYGEFRQIQSFGLIAVSLVGDAVGIGLDQKELTDYLRTKFRT
                     YLPGVRLEDVAENTDRFLRLVADRDRTIGNVTVRIWVIGNEYPVAYHVRFDAGNFSDP
                     AIWSEEILGHGSSKTAPGAIRKILDEMMQMFSRTFFQVRGREL"
     gene            complement(1918..2370)
                     /locus_tag="PLM79_14495"
     CDS             complement(1918..2370)
                     /locus_tag="PLM79_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87568.1"
                     /translation="MVLKNDFRKASLFVFFALAVMIPLSCSSVSKQKGEQEDLQEIAD
                     AFNSAVRWSDFKTACGFVLPPRKQAFWDQMDEMSNTVRIMDFQIREVSVPQKKNRGTV
                     ILRYRYYHMDSPNMRTKNLQQHWIFSEQEKSWFVAEHNLQSLMEGKKK"
     gene            2939..3364
                     /locus_tag="PLM79_14500"
     CDS             2939..3364
                     /locus_tag="PLM79_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HOV87569.1"
                     /translation="MDQKSLAFWMNHSTNFIEMALRTDKREALHQADGYGKCTRECGD
                     TLEIFLIVRDGRIRSASFQTHGCIYTVACANALVDLVEGKTTEEAWKISEEDIRDFLE
                     TLPDNEEHCAELAVKTLRLALVDLRQTERQPWKKFYPRR"
     gene            complement(3380..4042)
                     /locus_tag="PLM79_14505"
     CDS             complement(3380..4042)
                     /locus_tag="PLM79_14505"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOV87570.1"
                     /translation="MIGKNVIVVATRNRGKSKEIRSFLEAFPVEILDLNDFGPIPEVT
                     EDGETFEENAYKKAAFTAKVLGLPALADDSGLEVEALGGAPGVHSARYAGPGADDESN
                     NRKLLLNMREIRERSARFCCVLSLAVPTGPALTYTATCDGIILEEPRGSSGFGYDPLF
                     YYPPMGKSFGEMTLEEKSRVSHRGKALTELKNEFPQVLRWLDQRAEEEKTLRGLKDIC
                     AE"
     gene            complement(4071..4856)
                     /gene="tatC"
                     /locus_tag="PLM79_14510"
     CDS             complement(4071..4856)
                     /gene="tatC"
                     /locus_tag="PLM79_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOV87571.1"
                     /translation="MTATNDRMPFTQHLEELRKRMIVCAIAVAVGFLISYAFKESIFE
                     ILMKPWVEALPKGQSAKLIYTAPHEAFFTYLKVSLIAGFGLAVPVILFEFWRFIAPGL
                     YENEKRYLIPVVFFSSFFFLGGALFGYFIVFPVGFQFFTSFATEYITPMVSTKEFLTF
                     STRLLLAFGFVFELPIFAFFLAKLGLISSAFLRRQRRVAIVVIFIVAAALTPGPDVFS
                     QILMAVPLLILYEISIWIVHFAARKESALEPTEQKPAASDAGK"
     gene            complement(4917..5426)
                     /gene="tatB"
                     /locus_tag="PLM79_14515"
     CDS             complement(4917..5426)
                     /gene="tatB"
                     /locus_tag="PLM79_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOV87572.1"
                     /translation="MFGIGFPELLLIMAIALIVVGPSKLPDLARALGRGYAEFRRATN
                     ELKETFEQDETVREIKQEFHAAQHQIQFDKLYPDPNEAKEVLSSSAEISRTPLDPSPG
                     PESEATPEVPYGEETSDRTEDPVPLEDFMETASEQPQGPVPPSEGGPTAAGASPESPP
                     HDSNGPKSA"
     gene            complement(5487..5699)
                     /locus_tag="PLM79_14520"
     CDS             complement(5487..5699)
                     /locus_tag="PLM79_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOV87573.1"
                     /translation="MFGFGMFEFLLIVLILVLFCYGGKKFPQIGQDLGRSITEFKKGI
                     RGRSAAPKTPSEQSRQETGRSNPKSP"
     gene            complement(5715..6566)
                     /gene="ccsB"
                     /locus_tag="PLM79_14525"
     CDS             complement(5715..6566)
                     /gene="ccsB"
                     /locus_tag="PLM79_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOV87574.1"
                     /translation="MINSSILLSITTFAYLFCAVFYLAGVVFRHNPLLRVGSLVGLGT
                     LGIQTAGIVLRWVESYQIGYGHAPLSNLYESLVFAAWAIMLIYLVLEHRSKHRVLGIF
                     PALFAFLAMAYASFSTQVDSRIQPLVPALKSNWLIAHVIACFLGYAGFAVATGISILY
                     LVRKSRAANPHPRGILAALPEPRQLDELNYQMVLFGFLWLSIGIITGSVWANSAWGTY
                     WSWDPKETWSLITWLVYAALLHARSMQGWRGRRVAWLSLLGFGCVLFTYFGVNFLLSG
                     LHSYATK"
     gene            complement(6563..7954)
                     /locus_tag="PLM79_14530"
     CDS             complement(6563..7954)
                     /locus_tag="PLM79_14530"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOV87575.1"
                     /translation="MQQKNSAWRTFYEALASVKLSVFLFLLLAVCSILGTLLPQGLSV
                     EELQHRYKPSVVWWMETLSLHDLYHSGWFQILLVLLCVNLLVCSIQRFPKTLKLLQTR
                     DDPVDPAKLLKFGSSFEITTRLPWREAQARLEGIIPGELAPLRLAPGKNGFAAVAEKG
                     RWSPLMVYGVHFSVLLILSGALIGSFFGFKGMMNIPEGQLSDEVLLYQRRQSLLLPFQ
                     VRCDDFDVSLYDTGAPKEYRSDLVILDEGKEALRQTIRVNDPLTYRGVTFYQASYGLI
                     LKSADVELTDKETGKTYTLSLPYREMVRIPGTQDQIEAIQFQQNFSRFGPAVGIVLLR
                     EGQTEPMGSWILVKMPEFHGNRVQNYQVKITKTEEGYYTGLQVKQDPGVWIVYAGFIL
                     LLAGIGITFYTRHRKLWIWADAGGPESSPVRIVVAGRTNKNAVTFEEEFKHLCGRLQL
                     ELATGSGDEGTKR"
     gene            complement(8206..8676)
                     /locus_tag="PLM79_14535"
     CDS             complement(8206..8676)
                     /locus_tag="PLM79_14535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HOV87576.1"
                     /translation="MSVSAKRPMGLGDPIGRAARVTAAIIRRTGRFFLVQRPPEKSFG
                     LFWEFPGGKVEPGESLEESLQREIREELCWDIDVERPFHTVACRVGDLHIELYAYWCR
                     IRGGDLFLREHLSFYWAYPEEFRHFLFTRADQELAEVLATGSRGSLGPKMPSRR"
     gene            8895..9815
                     /locus_tag="PLM79_14540"
     CDS             8895..9815
                     /locus_tag="PLM79_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOV87577.1"
                     /translation="MDDNRINYDVVILGSGPAGLQAAIHASRAKVKVAVLGRIHKSSL
                     YRAHVENYCCTAHSTGEEILVQGREQARAFGAELLDEDVLGLSRGQGGEFLIDLESGK
                     RLAARAMILAMGISRNRLNVPGEKEFLGKGVSYCVECDGNFFRNQSVAVVGNESAACS
                     GALTMLLLAKEVHLVYEKLQVSEHLRYQVENSGIVLHPGRRVREIQGTQGVEAILLDD
                     GQRIEVYGVFIELGAKGALELAAGLDVALDSENMRYIAVNRKQETNVAGVYAAGDICG
                     PPWQMAKAVGEGCVAGLEAASYAKRVRPKP"
BASE COUNT         2222 a         2860 c         2947 g         2031 t
ORIGIN      
        1 agtacacttg ttgctccgcc agcctctcca ggttgtcttt ctgcatgaaa aacccgtgct
       61 cgatggagtg acatcccgct ttgagggcca tggacaccgg ccctttgccg tttgcgtgaa
      121 ccatggtctt ccgtcccaat tcctcacccc gtcgaacggc catccggagt tcgtccaggt
      181 taaattgcgg caaggtctct ttcccaaatt cccgaagact gttcagtccc gagttcacga
      241 tcttcacgtg atcggcgctt cgagcatacc gtgcgacaca atcggccaga gaacttccct
      301 cggggggagg atcgccgata agtcttccgt atctgccggg agaccgccag gccttgcccg
      361 gggacttcag acggacgggg acacaatcct ctttcatcca tcgcttctta taccaaagag
      421 tatgcccgtg agcgtccccg ccgtctctga ccgccacgat gcctcgggag gcatggtgcc
      481 ggaggtgttc ctcgatgacc ggcttgactt cctcgaaggt acgggaaagt tggtgctctc
      541 gttccccgcg gtccggggtc ccggacatgc tcaaatgaac atgacaatcg atcagccccg
      601 gaatcagagt gcagtcggac aggtcccaca ccctgcggtg agacttgtct tcatagggat
      661 cgtgccctct cgatccgttc catcccagtt cagtgacgaa accgttttcc atctcgagaa
      721 agacgtcccg gcggatggaa cccgaagatc cgtccatgag ccacccggcc cgaaccatgt
      781 ctttggattt caagaacttt cgcctcgcgt caaaagggtt tccggatccc gtttgtcgtc
      841 gccgttggga aggttcgagg gggccgttgt cccccccggg ctacaactcc cgaccccgca
      901 cctggaagaa ggtccgtgag aacatttgca tcatttcatc gaggatcttc cgaatggccc
      961 ccggagccgt cttgctggat ccgtgtccca ggatttcctc ggaccagatg gcaggatcgc
     1021 tgaaatttcc ggcgtcaaaa cggacatgat aagccacggg gtattcgttc ccaattaccc
     1081 atatgcggac cgtaacgttg ccgatcgtcc tgtccctgtc ggcaacgagg cggaggaatc
     1141 gatccgtgtt ctcggccacg tcttccagcc gcactcccgg cagataggtc cggaacttcg
     1201 tcctcaggta gtccgtaagc tccttttggt cgagcccgat cccgacggcg tcgccgacca
     1261 gggaaacggc aatcaagccg aagctctgga tctgccggaa ttctccgtaa aggatcaaag
     1321 cttccttgtc cgtaagatcc ccggttttcc cggggctgca gaaaccaaac ccaggaaatg
     1381 gacagcccaa aaacacaaga aggagaagca cgctccctgc cggcccaggg aaccgcagga
     1441 acgagcaacc cccgtggggg cgaaagcctg cgtccgggca attggccggg gttgcggttt
     1501 ccaccgcaac ggcccgtcct ttgagagccc tcgatctcac ccgctcggag tgtggcgagg
     1561 tgctttgcag gtcccggata caacggccga aagaaccttc ttcccgtctc atgactgcat
     1621 gccgtggcta ctcgatgtgg gaaagaaggc ggagaagatt ctccccccgc tgatcagccc
     1681 cctcggctgt ggttccggag gggagaacac gcccggaaca ttccggggga cctccttggg
     1741 ccgaggatgc ccacggcgaa acccctggaa cctttgagcc gattggagac ttgacgggtc
     1801 gtgaggcccg cctccgtcac gattccgtgg aaagaaatct ctccggagag ctggggaagt
     1861 ctcgaagatt gcgtgaaacc atcattgggc tggaggatgc actctattcc ttcaccgcta
     1921 cttcttcttt ccttccatca agctttgaag attatgctcc gcaacaaacc acgatttttc
     1981 ctgttcggaa aagatccaat gctgttggag gtttttggtc ctcatgttcg gactgtccat
     2041 atgataataa cgataccgga ggatgacggt ccccctgttc ttcttctggg gaacggacac
     2101 ctcccggatc tggaaatcca tgatacgaac cgtgttgctc atttcgtcca tttgatccca
     2161 aaacgcctgt ttccgggggg gcaaaacgaa accgcaggcg gtcttgaagt ccgaccaccg
     2221 aacggccgaa ttgaaagcgt ccgcgatctc ctgaaggtcc tcctgttccc ccttctgctt
     2281 gctgacgctg gagcaggaaa gcgggatcat gacggcaaga gcgaaaaaga caaacagcga
     2341 cgccttcctg aaatcattct tcaaaaccat gccgcgattc tccttattcg gtctctcgaa
     2401 tccggggata aacccagctc aatcgttccc gcattgtcaa ccatgcggaa ttttgaatca
     2461 agccccaatt gtgtacgagt tcggcggggt ccgttgccga aggcgcagtc tgttcggtca
     2521 ccgccgcttc ttctttcctt ccatcagacc ttgagaagat cgtgctccgg aacaaaccgc
     2581 aattcttcct gtttggggaa ggtccgcggt ctttccgggg tcaaaggtgg gagggcctga
     2641 ccacgagcac gggacaggct ggttgagaaa cgggcatccg ggtccctccg ggcgagttcc
     2701 ggacgtctgt cggggttgtt tgacggacct tgcggtccca gagcgtttgg agaacgggcc
     2761 gacacgactc tgtccagagg tttgtcgcaa aggtccccca caacctctgc ttcgggatga
     2821 tttgaaaaga cagggggttc cggctaaaat acccgatgta ggctttcaaa ggcgagtctt
     2881 tgctcgaatt gggtttcgaa gctcatcttc tgcgcaaacc gtgtaaaagg aagtagggat
     2941 ggatcagaag agtctggcat tctggatgaa ccactccaca aacttcatcg aaatggcgct
     3001 gcgaacggac aagcgtgagg ccctccacca ggcggacgga tacggcaaat gcacccgtga
     3061 gtgcggagac actctcgaga tctttctgat cgtccgagat ggccggatcc ggtcggcatc
     3121 ctttcaaacc cacggctgca tctacacggt tgcatgtgcc aacgccctgg tggatctggt
     3181 ggagggaaaa accacggagg aagcctggaa gatttctgag gaagatatcc gggattttct
     3241 cgagacgctg ccggacaacg aagagcattg tgcagagctg gccgtaaaga ccctgcggct
     3301 tgccttggtg gacctgcggc aaacggaacg ccaaccctgg aagaagttct atccgcgaag
     3361 gtagagaaac ccctcggctc tactccgcac aaatgtcctt gagcccccgc agggttttct
     3421 cctcctccgc ccgttgatcc agccagcgga ggacttgggg gaactcgttt ttcagctccg
     3481 tgagagcctt accccggtga ctcacccttg acttctcctc gagggtcatt tctccgaagc
     3541 tctttcccat gggagggtag taaaagagcg gatcgtaccc gaaaccgctg gaaccccggg
     3601 gttcctcgag aatgattcca tcgcaggtcg cggtgtaggt gagggccggt cctgtgggca
     3661 cagccaggga aagcacgcag caaaagcggg ccgaccgttc ccggatctcg cgcatgttga
     3721 gcaggagctt gcgattgttg ctttcatcat cggccccggg tcccgcatag cgggcggaat
     3781 gcaccccggg ggcccccccc agagcttcca cttcgaggcc ggaatcgtcg gcaagggcgg
     3841 gcaaccccag aaccttggcg gtaaacgccg ctttcttgta tgcattctcc tcgaaggttt
     3901 ccccgtcttc cgtcacttcc ggaatggggc caaaatcgtt gagatccagg atctccaccg
     3961 gaaaagcctc caggaaactg cgaatttcct tggacttgcc acggtttcga gtcgccacga
     4021 cgatgacgtt ctttccaatc attcttcagg tcctcatgga aacgaggggt ctactttccc
     4081 gcgtccgatg cggccggttt ttgctctgtg ggttccaggg ccgattcctt cctggcggca
     4141 aagtgaacaa tccaaatact gatttcataa aggatgagca aagggaccgc catcaggatc
     4201 tgggaaaaga cgtcgggacc gggagtcaag gccgccgcga cgatgaatat gaccacgatg
     4261 gccactcggc gctggcgtcg caaaaaggcg gagctgatga gtcccagctt ggccaggaag
     4321 aacgcaaaaa tcggcaactc gaatacgaac ccgaaggcaa gaaggagcct ggtggaaaag
     4381 gtgagaaact ccttggtgct caccatgggc gtgatgtatt cggtagcaaa agaggtaaag
     4441 aactgaaatc ccaccggaaa tacgatgaaa tagccgaaga gggctccccc gagaaagaag
     4501 aaactggaga agaacaccac gggaatgagg tatcgcttct cgttttcata gaggccgggg
     4561 gcaatgaatc gccagaactc aaaaaggatt acgggaaccg caaggccaaa gcccgcaatg
     4621 agggaaacct tcaagtaggt gaaaaacgcc tcgtgggggg ccgtatagat gagcttggcc
     4681 gattgccctt tggggagggc ttccacccat ggtttcatga ggatttcgaa gatgctctct
     4741 ttgaaggcat agctgatgag gaaaccgacg gcaaccgcaa tggcgcagac gatcatccgc
     4801 tttcgaagct cttccagatg ctgggtaaaa ggcatcctgt cattggtggc ggtcatggtt
     4861 cgactcactg ttggaactga aggagagacg gcacgggctg cagcgacgac gggggctcat
     4921 gcgctcttgg gtccgttcga atcatgggga ggactctccg gggacgctcc cgccgcggtc
     4981 gggccgccct ccgagggggg gaccggtcct tggggctgtt cgctggccgt ttccatgaag
     5041 tcctccaagg gtacggggtc ctccgtccgg tcactcgtct cctcgccgta gggcacctcc
     5101 ggagttgcct ccgattccgg cccgggtgaa ggatcaaggg gggtccgcga gatctccgcg
     5161 gaggaactca aaacttcttt ggcctcattg ggatcggggt aaagcttgtc gaactggatc
     5221 tgatgctgag ccgcgtgaaa ctcctgcttg atttcgcgga ccgtctcgtc ctgctcgaac
     5281 gtctccttga gttcgttggt cgctcttcga aattcggcgt agcctcgccc caaggcgcgt
     5341 gccaggtcgg gaagcttgct cggccccacc acgatcaggg cgatggccat gatcagtaac
     5401 agttccggga acccgatgcc gaacatggtt ggttggctcc ttgttgtttg agaacagacc
     5461 ggcgaacggg ccgcaggccg tctctcctaa gggctcttgg gattgctccg gccggtttcc
     5521 tgccgacttt gctccgaggg ggtctttggg gcggcggaac ggcctcggat tcccttcttg
     5581 aattccgtaa tgctcctgcc caaatcctgc ccgatttggg gaaacttctt tcccccgtag
     5641 caaaaaagca ccaggatgag cacgatcaac aagaactcga acattccaaa cccgaacacc
     5701 actcacctcc ccgctcactt agtagcgtaa ctgtgcaacc cgctcaacag gaaattgact
     5761 ccaaagtagg tgaagagcac acacccaaaa cccagcagag agagccacgc cacgcgtctt
     5821 ccccgccagc cctgcatgga acgggcatgc aataaggctg cgtagaccag ccaggtaatg
     5881 agcgaccacg tctctttcgg gtcccagctc caataggtcc cccaggcgga attagcccag
     5941 acggaacccg tgatgatccc gatggacagc cagaggaagc cgaacaggac catctggtag
     6001 ttgagttcgt ccagttgccg cggctcgggc aatgcggcca ggattccccg gggatgggga
     6061 ttcgccgctc gggacttgcg aacgaggtag aggatgctga tgccggtggc aacggcaaaa
     6121 cccgcgtaac ctagaaaaca cgcaatgacg tgggcgatga gccagttgct cttgagagcg
     6181 ggcaccaggg gttgaattct cgagtccacc tgggtggaaa aagacgcata agccatggcc
     6241 aggaaggcaa agagggcggg aaagatcccc aggacccggt gcttggaacg atgctcgagg
     6301 accaggtaga tgagcatgat ggcccaggcg gcgaacacca gggactcgta gaggttggag
     6361 aggggagcat gtccgtaccc tatctggtag gattcgaccc aacgcaacac tattcctgcg
     6421 gtctgaattc ccagggtccc caagcccacc aggctgccca ctctcaggag agggttgtgg
     6481 cggaacacta ccccggccag gtaaaacacc gcacagaaca ggtaggcgaa agtcgttatg
     6541 ctgagaagga tggaactgtt gatcatcgtt tcgttccctc atcccccgat ccggttgcga
     6601 gctcaagttg cagacggccg cacaggtgtt tgaattcctc ttcgaacgtc acggcattct
     6661 tgtttgttct tccggccacg acaatcctga cgggagaaga ctcggggccg cctgcatcgg
     6721 cccagatcca gagtttgcgg tgccgagtgt agaaagtgat tccgatcccc gccaacagca
     6781 aaataaaccc ggcatagaca atccagaccc ccggatcctg tttcacctgg aggccggtgt
     6841 agtagccttc ctcggtcttg gtgatcttca cctggtagtt ctggacgcgg ttgccgtgaa
     6901 actccggcat cttcaccaag atccacgatc ccatgggttc ggtttgtcct tccctcaaga
     6961 gcacgatgcc cactgcaggt ccgaaacggc tgaaattctg ttgaaactga attgcctcga
     7021 tttgatcctg ggtgccggga atccggacca tctccctgta cggaaggctc agagtatagg
     7081 tcttcccggt ttccttgtcc gtcaactcga cgtccgccga tttcaggatc aacccgtagg
     7141 aggcctggta aaaggtcacc ccgcggtaag tcagagggtc gttcacccgg atggtttgcc
     7201 gaagggcctc tttcccttcg tcgaggatga ccaagtccga acggtactcc ttgggggccc
     7261 cggtgtcgta gagagagacg tcaaagtcgt cgcagcgcac ctggaacgga aggagcagag
     7321 actggcgacg ctgatagaga agaacttcgt cggagagctg tccttccggg atgttcatca
     7381 tccctttgaa tccgaaaaag gagccgatga gagcgcccga caggatcagc aatacgctga
     7441 agtgcacccc gtagaccatg agggggctcc atcgaccctt ttcggcaacg gccgcaaacc
     7501 cgttctttcc cggcgcgagt ctcaagggag ccagctcacc cgggatgatt ccctcgagcc
     7561 gggcctgcgc ttcccgccag gggagcctgg tggtgatctc gaaactggaa ccaaacttca
     7621 aaagcttggc ggggtccacg gggtcgtccc gggtttgaag gagcttcagg gtcttgggaa
     7681 agcgctggat ggagcatacc aggaggttga cgcaaaggag aaccaacagg atctgaaacc
     7741 atcccgaatg gtacaagtcg tgaagactca gggtctccat ccaccagaca acggacggct
     7801 tatagcggtg ctgaagctct tcgacgctga gcccttgagg cagaagagtt cccaagatgg
     7861 aacaaacggc cagaaggaga aaaaggaata cgctcagttt cacggaagcc agcgcttcat
     7921 aaaacgttcg ccaggctgaa ttcttctgct gcatgaggct ctcttctttg aaaggctctc
     7981 gggtggcggc ttcccgtccc ggaagggcgc gctctgccct gtccgatctg gagaaaaaac
     8041 cgtccattct taacctttta taggtcgcct tgacaagacg aaggaacaaa agagctccgt
     8101 cttcccggcg gtttggcgga tcatcgggac cggggttccc tcgaaagaat gcgggaccca
     8161 ttcccggcaa gaaaaatact ccccggttct acgatcaacc cggattcatc gccgggaagg
     8221 catctttgga ccaagagacc cgcgggatcc cgtcgcgagt acctcggcca gctcctggtc
     8281 cgcccgggta aacaggaaat gacgaaattc ctcgggatag gcccaataga aggagaggtg
     8341 ttcccgcaag aaaaggtccc cgccccggat gcgacaccag taggcgtaaa gctcgatgtg
     8401 aaggtcgccc acccggcagg cgaccgtgtg gaaaggcctc tccacgtcga tgtcccaaca
     8461 gagttcttcc cgaatttctc tttgcaaaga ctcttcgagg ctttcccccg gctccacctt
     8521 gcctccggga aattcccaga agagcccgaa gctcttttcc gggggtctct gtacgaggaa
     8581 gaatctgccc gtacgccgaa tgatggccgc cgtgacccgg gccgcccgcc cgatcgggtc
     8641 tccaagaccc atgggcctct tcgctgaaac actcatgatc ccgggccccc tccttcccct
     8701 ccgccagcct cttcggccct tgtcgaacaa accccaaagt aatcccgaat gcgcgaaaaa
     8761 ggtaccccgg atttcctccg gcccttatat tgatggaatc ctggaacgag gaatggtccc
     8821 caagatcaat gggacagcta aatctttggc gcggtcggag gttgacgaat cgtgggtccg
     8881 aggaggtgga cgaggtggat gacaatcgca taaactacga cgtggtgatc ctggggagcg
     8941 ggcctgcggg actgcaggca gccatccatg cttcccgggc aaaggtgaag gttgcggtcc
     9001 tgggccgcat ccacaaaagc tcgctctaca gggctcatgt ggagaattac tgctgtacgg
     9061 cccattccac gggggaagaa atcctggtgc agggaaggga gcaagcccgg gccttcggtg
     9121 ccgaactcct ggacgaggac gtgttgggcc tgagccgagg tcaaggtggg gagttcctca
     9181 ttgacctaga gtcgggaaag aggcttgcag cccgggccat gatccttgcc atgggcattt
     9241 cccgaaaccg gctcaacgtg cccggggaga aggagtttct gggcaagggg gtcagctatt
     9301 gcgtggagtg tgacggcaac ttctttcgca accaatcggt ggcggtggtg ggaaacgaaa
     9361 gcgccgcctg ttccggggca ctgaccatgc tgcttctggc aaaggaagtc cacttggttt
     9421 atgaaaaact ccaggtgagc gaacatcttc ggtaccaagt ggaaaacagc ggcatcgtcc
     9481 tgcatccggg ccgccgggtc cgggagattc aggggaccca aggggtcgaa gcgatcctcc
     9541 tggatgacgg acaaaggatc gaggtctacg gggtgtttat cgaactgggg gccaagggtg
     9601 ccctggagct tgccgccggg ttggatgtgg ctctggattc tgaaaacatg cgctatatcg
     9661 cggtgaacag gaagcaggag accaacgtgg cgggcgtgta tgccgccgga gacatctgcg
     9721 ggcctccgtg gcaaatggcc aaggcggttg gggaaggctg tgtggcgggt ttggaggccg
     9781 catcctacgc caagcgggtg cggccgaaac cctgatgttt tttttcttga ctccgggccg
     9841 caaatgagta gaatcccgct tcgcttcagg gcgcgcggtg caccaaaaaa aagatgttga
     9901 cagaggaaac gcaccgcagt ataaagagcg tctgcccgca aggaaggagg gtcccgaagg
     9961 aaaaagatgg acccccggga agcggaaaag ggaatgaaaa atttcccctg ttctttggaa
    10021 actaaatagt gggtcgaagg tgctgggcct tttagggaag
//
LOCUS       DAOOVC010000141         9936 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4324_length_9936_cov_5.207368, whole genome shotgun sequence.
ACCESSION   DAOOVC010000141 DAOOVC010000000
VERSION     DAOOVC010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4324_length_9936_cov_5.207368"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLM79_14545"
     CDS             <1..662
                     /locus_tag="PLM79_14545"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOV87578.1"
                     /translation="AAHRGALKARGRTLAVMGCGLDVDYPRSNAALREEIALSGALLT
                     EFVLGTPPAPGHFPQRNRIISGLALGVVVVEAAHRSGSLITARLALEQGREVFAVPGM
                     ARHFRSIGPHRLLKEGAKLVESAEDILEEIAPLVRRSPESVSRERSDVPAQPLNEDES
                     ALWKVMGESPKHIDEISRDLRWPVSRVTSHLLAMELKGIVRQLPGKYFMREAKRGNLN
                     G"
     gene            655..2970
                     /gene="topA"
                     /locus_tag="PLM79_14550"
     CDS             655..2970
                     /gene="topA"
                     /locus_tag="PLM79_14550"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOV87579.1"
                     /translation="MAKSLLIVESPTKAKTLGRYLGKQFIVKASVGHIKDLPKNKLGI
                     DVEHDFHPDYQVIRGKKKVIHDLSEAAKNAEAIYLGPDPDREGEAIAWHIADEVRVEN
                     KPVYRVLFYELTRGAIQKALENPLALNRSLYEAQQTRRILDRLVGYLISPILWQKVKR
                     GLSAGRVQSVALRLLCERENEIRSFESQEYWTIEGRMVPGTAPGEAVEGQEKPRSFSA
                     QLARCEKKKCRIPDVEEANRLLEALRPLSYTVQKVEKKKRTKSPPPPFITSTLQQEAA
                     RKFHSSARRTMSIAQKLYEGVEMGDEGAVGLITYMRTDSTRLSQEAVQAARALIEEVH
                     GKDYLPAKPAVYKAKGRAQDAHEAVRPTDVRRTPEQAARYLSKEEARLYALIWKRFVA
                     SQMAPALYAQTTVDIVGGDYEFRAVGSVLEFDGYQVLYTESHDNGEGSDKDDAWLPEL
                     KEGQPVLLEELIPKQHFTQPPPRFTEASLIRELEELGIGRPSTYATIVSTILDREYCR
                     VENRRLLPTELGWLINNLLVKNFPDIVDVEFTARMEKSLDEVESGQYPYLKLLEDFYS
                     QFARSVREAESSMLNLKTAGWPTSEPCPTCGRPLHIRLSRNGPFVACSGYPKCPFSSD
                     FQRDEKGGIVLVAEAETGEFCEKCNRPMVHRKGRFGTFLACSGYPACKNTRSPRTGIP
                     CPREGCTGELVERVGKGGRHFYGCNRYPECKTAFRDHPVATPCPQCGSPILLEKVMKS
                     GVAKRVCPRPDCKYEERSRAGSGKEKREGAV"
     gene            2967..3761
                     /locus_tag="PLM79_14555"
     CDS             2967..3761
                     /locus_tag="PLM79_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOV87580.1"
                     /translation="MNLHPNLHHLLQSFAFVVGCCLGSFFNVIIHRLPGKGSIVHPGS
                     HCPRCGHPIAFYDNIPLLSFLVLGGRCRHCRERISLRYPLVEVLTGVLTLVLFRTYGP
                     LPQFFIETLFVYLLILIAFIDLDTYLIPDVLSLSGIVAGLALSFFTPRLSWVDSLLGI
                     LLGGGFLYLIAVGYQVLRHKEGMGGGDIKLLAMIGAFVGLPGVVFTVLAASVVGTAAG
                     VWVMVRSRQGLSAMLPFGPFLSLGAVCHLFWGQTFFSWYLERFTGG"
     gene            3877..5199
                     /locus_tag="PLM79_14560"
     CDS             3877..5199
                     /locus_tag="PLM79_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HOV87581.1"
                     /translation="MRYPQIKTVLPGPRAAELIAVDREFVSPSYTRIYPLVAESAQGL
                     WITDPDGNVFLDFTAGIAVCATGHCHPHVVEAVQRQVGRLLHMSGTDFYYTPQIHLAR
                     KLTSLATGAGPRKVYFGNSGAEAVEAAFKLARWHTRRELNLAFFGAFHGRTMGALSLT
                     ASKTIQKKHYNPLVPGITHVPYPYCYRCPHNLCYPSCGIACVTWIEDTLFRTTVPPEE
                     VAAIFVEPIQGEGGYIVPPPEFHRELFRVARNYGILYVADEVQSGMGRTGKMFAMEHF
                     SVAPDILALAKGIASGLPLGATLARSRIMDWEAGSHASTFGGNPVSCQAALATIELLE
                     NGLMENAAQRGQQLKAGLEELQEIYEPIGDVRGLGLMVGMELVKDRETKERAGEWRDA
                     LIQAAFRKGLLLLGCGQNTIRFCPALTVSAREVSVCLEILESVLRELF"
     gene            5456..7249
                     /locus_tag="PLM79_14565"
     CDS             5456..7249
                     /locus_tag="PLM79_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOV87582.1"
                     /translation="MKGKSVIAGVVVVVIALVLLLPALPISWFGETRGSLFAKPTPAD
                     TVIHNGSILTSNDRRPYARALAIRDGKIVYIGSDNGKALSFVGTGTRVIDAKKSLVTP
                     GFVDAHTHALWLGILSPVMTFIYDAGSLEDVARLVRSYGEQNPDLPFIFAIGWKYDYI
                     PNGIPTLRMADDILRDRPLILWSHDGHTGWVNSLALQQMQERNPKAFEYLTPDFDKDH
                     KPTGIFRHFYTINPMDFYTVEEMGGEAIVRKMAQGVAAKLEEGLRVGVTCHHDVMIHR
                     STFDLLLKLKKRGVFRDARVRASHFVNHHLYEDDPATLFLNLASWKRLAGSSTDHFVI
                     GESIKLGIDGVSPNHTALLYKPYKDDKTTKGVASYTAEGFNRLVTLIDSMRLQICTHG
                     VGDAGITRIIDGYEFARKVNGAWDSRHTIEHNSMIVDKDVPRLARLGIHASMQPTHYF
                     GDRATVKALGKRRFLHAHRFGTLQRAKVRLAFGTDYSIVPMNPIYGTIVAVTRLSCLG
                     PPRTMDEFKEMLSFEDVIRHYTLGSAAALHMDRLIGSLEVGKRADLVIFSIDRNEFSR
                     MCNLGIMPPSDILDQLVSMTFVDGRVVYQRP"
     gene            7696..8424
                     /locus_tag="PLM79_14570"
     CDS             7696..8424
                     /locus_tag="PLM79_14570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV87583.1"
                     /translation="MSEDHPEWLFDEARHTGVDYANREVVADYDRQHQGFRDFEKEAE
                     MIVAALDLSENSTVLDVGCGTGGLSVCLARKCKQVYAVDVSQAMIEVLEGKIEAQGLG
                     NITPQRAGFLTYRHRAEAPDAVVVNIALHHLPDFWKQIALCRLHDLLKPGGKMFLADV
                     VFGFDPRTYEEAVEGWLHCMRDLAGPRMADETIVHIRDEFSTWDWVMSGMLERAGFRL
                     EQCLDLMPQMRAYICSKPGEQEPR"
     gene            8619..9401
                     /locus_tag="PLM79_14575"
     CDS             8619..9401
                     /locus_tag="PLM79_14575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOV87584.1"
                     /translation="MTCKCFFVEQADTRGGPVTFTGATAHHMERVARLRVGDTIELRD
                     GQGNAWLGSIDAMGRGAVSVRILGDAEGHRESPLELTLAMAFSRADRMDWSLRQAVEI
                     GIHRFVAFRSRRSQYGLNEKEAAARRQRWWKIAREAMCQCGRVKIPEINILPDLDRYV
                     SQTVHEAAGPSEVLKIVACEEGPRRGLLDLWSQTPWCKQFHAVIGPEGGWSPEDLELL
                     FRAGFQSVHLGPRVLRMETASTVLLGAVQLLWGDFHVAQEGV"
     gene            9404..>9936
                     /locus_tag="PLM79_14580"
     CDS             9404..>9936
                     /locus_tag="PLM79_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87585.1"
                     /translation="MRCHKCGYHSFDYLSECKMCGSDLSSIRDSLGLLSVKPDIPFFL
                     NVLLRGPGAGETAFPTPEDLAATIAEIDLGPELDLDAPLEDFAGTLPQAPPGPAPAQA
                     QTEEIELVLEDLKIAEEEPMIELSDADLDQLSDREQMEGLDLKVDLDLDAEPPLKGLS
                     QAAPGEAAREEPEVGILS"
BASE COUNT         1958 a         2974 c         3143 g         1861 t
ORIGIN      
        1 gcgcggccca ccggggggcg ctcaaggctc ggggacgaac cctggcggtg atggggtgcg
       61 ggctggacgt ggactatccc cggagcaacg ccgccctccg ggaggaaatc gccctttcgg
      121 gggcactact gacggaattc gtattgggga cgcccccggc accggggcat ttcccccaga
      181 gaaaccggat catcagcgga ctggccctgg gggttgtggt ggtcgaggcg gcccatcgga
      241 gcggctccct catcaccgcg cgcctggccc tcgaacaggg cagggaggtg ttcgcggttc
      301 cgggcatggc gcgtcacttc cggagcatcg gtccccaccg gttgctcaaa gagggggcga
      361 aactggtgga atcggccgag gacatcctgg aggaaatcgc ccccctggtt cgacgcagtc
      421 ccgaatccgt ttcccgggaa cgttccgacg ttcccgcaca gcctttgaac gaggacgagt
      481 cggccttgtg gaaggtcatg ggcgagagtc ccaaacacat cgacgaaatc tcccgggatc
      541 ttcggtggcc cgtttcccgg gtgacctccc atctcctggc catggagctc aaagggattg
      601 tgcgccagct accgggtaag tactttatga gggaggccaa gagaggaaat ctgaatggct
      661 aaatcgttgt tgattgtcga atcccccacc aaggcgaaaa ccctgggacg atacctggga
      721 aaacaattca ttgtgaaggc gtccgtcgga cacatcaagg atctccccaa aaacaagctg
      781 ggcatcgatg tagaacatga tttccacccc gactaccagg tcatccgggg aaaaaagaag
      841 gtcatccatg acctgagcga ggctgccaag aacgccgaag cgatctacct ggggccggac
      901 cccgaccggg agggggaggc cattgcgtgg cacattgccg acgaggtccg agtcgagaac
      961 aagcccgtct accgagttct tttctatgag ttgacccggg gcgccattca gaaggccctc
     1021 gagaaccccc tggccttgaa ccgttccctg tacgaggccc aacagacccg gaggatcctc
     1081 gatcgcctgg tgggatatct catttccccc attctctggc agaaggtaaa gcggggattg
     1141 agcgccgggc gggtccaatc ggtggccctg aggctccttt gcgaacggga aaacgaaatc
     1201 cgcagcttcg aatcccagga gtactggacc atcgagggaa ggatggtccc gggaaccgcc
     1261 cccggggagg cggtcgaagg gcaggagaag ccccggagct tttccgccca gctggcccgg
     1321 tgcgagaaga agaaatgccg gattcccgac gtcgaagaag cgaatcgact gctcgaggcc
     1381 cttcgacccc tctcctacac ggtgcagaag gtggaaaaaa agaagcggac aaagtccccg
     1441 cctcccccct tcatcaccag caccctccag caggaggcgg caagaaagtt tcattcttcc
     1501 gccaggcgca ccatgtccat tgcccagaag ctctacgagg gggtcgagat gggggatgaa
     1561 ggagcggtgg gcctgattac ctacatgagg accgattcca cccggctttc tcaggaagcc
     1621 gtccaggcgg ccagggccct catcgaggaa gtccacggga aggactacct cccggccaag
     1681 cctgcggtct acaaggcaaa gggccgggca caggacgctc acgaggccgt tcggcccaca
     1741 gacgtgcggc gaacccccga acaggcggcc cggtatcttt ccaaggagga agcccggctc
     1801 tatgccctca tctggaagcg gttcgtggcc agccagatgg caccggccct ctacgcccag
     1861 accaccgtgg acatcgtcgg aggagactac gagtttcgag ctgtcgggtc cgtgctcgag
     1921 ttcgacggtt accaggtgct ctataccgag agccacgaca acggagaggg gtccgacaaa
     1981 gacgacgcat ggcttcccga actgaaggaa ggtcaaccgg tactcctcga ggaattgatc
     2041 cccaagcagc atttcaccca gccgccgccc cggtttaccg aagcgagcct catccgcgag
     2101 ctcgaagagt tgggaatcgg acgacccagt acctatgcaa ccattgtctc gaccatcctg
     2161 gaccgggaat attgcagggt ggaaaaccgg cgcctgctcc ccacggaact gggctggctc
     2221 atcaacaacc tcctggtcaa gaatttcccg gatattgtgg acgtggagtt taccgcccgg
     2281 atggagaaga gcctcgacga agtggagagc ggccagtatc cttacctgaa gctcctggaa
     2341 gacttctaca gccagtttgc ccgaagcgtt cgggaggcgg aatcgagcat gttgaacctc
     2401 aagacggcgg gctggcccac cagcgaaccg tgcccgacct gcggccgccc tttgcacatt
     2461 cgattgagcc gaaacgggcc gtttgttgcc tgcagcgggt acccgaagtg cccgttctct
     2521 tcggatttcc agcgggatga aaagggaggc atcgtcctgg ttgcggaagc cgagaccggg
     2581 gagttctgcg aaaagtgcaa ccggcccatg gtccatcgca agggccggtt cgggaccttc
     2641 ctggcctgca gcggctaccc cgcctgcaaa aacactcggt ctccacgcac gggaattcct
     2701 tgtccccggg aaggctgtac cggtgagttg gtcgaacggg tagggaaggg ggggaggcat
     2761 ttttacggct gcaaccggta tcccgagtgc aaaacggcat tccgggacca ccccgtggcg
     2821 acgccctgcc ctcagtgcgg aagccccatc ctgctcgaga aggtcatgaa aagcggtgtt
     2881 gccaagagag tctgcccgag gcccgactgc aaatacgagg aacgctcgag ggccgggagc
     2941 ggcaaggaaa aaagggaggg ggcggtatga acctgcaccc caatctgcat cacctgctgc
     3001 aatcctttgc cttcgtggtc ggctgctgcc tggggagctt tttcaacgtc atcattcatc
     3061 ggctgcccgg caagggctcc atcgtccatc ccggttccca ttgcccccgt tgcggacacc
     3121 ccatcgcctt ttacgacaat atccccctgt tgagcttcct ggtcctggga ggccgctgcc
     3181 gccactgccg ggaacgaatc tccctgcgct atcccctggt ggaggtcctc accggggtcc
     3241 tcaccctggt cctctttcgc acctacgggc ccctgcccca attcttcatc gagacccttt
     3301 tcgtctacct tttgatcctc attgccttca tcgacctgga cacgtacctc attccggacg
     3361 tgctttcatt gagcggcatc gtggcggggt tggccctgtc tttcttcact cctcgcctct
     3421 cctgggtgga ttcgctcctg ggaatcctgc tcgggggcgg gttcctgtat cttatcgccg
     3481 tgggctacca ggtcctgcgg cacaaggagg ggatgggcgg gggcgacatc aagctcctgg
     3541 ccatgatcgg cgcgtttgtg gggctgcccg gagtggtttt caccgtcctg gcggcttcgg
     3601 tagtgggaac ggctgcgggc gtttgggtca tggtccgatc caggcagggt ctgagcgcca
     3661 tgctcccctt cggccccttc ctttccctgg gggcagtctg ccatcttttc tggggacaga
     3721 cctttttctc gtggtatctg gaacggttca cgggcgggta aggaattttc tcccgcccgg
     3781 tccgcccgga tggggtccct tctcccggcg cttcttccac ggatcgattt ccccggaaaa
     3841 ggacctcgaa tcttctcagt tcatggagaa atcaccgtgc gatatcccca aatcaagacc
     3901 gttctgccgg ggccccgggc ggcggagctc atcgccgtcg accgggagtt tgtctcccct
     3961 tcctataccc ggatttatcc cctggtggcc gaatccgccc aagggctgtg gattacggac
     4021 cccgacggca acgtctttct ggatttcacc gccgggatcg ccgtgtgcgc caccgggcac
     4081 tgtcatcccc acgtggtcga ggccgtccaa cggcaggtgg gacgcctgct ccacatgtcg
     4141 gggacggatt tctactacac cccccagatt cacctggcac gaaagctgac gagcctggca
     4201 accggggcgg gtccccgaaa ggtctacttc ggcaattcgg gggccgaagc agtcgaagcg
     4261 gccttcaagc tcgcccggtg gcacactcgg cgcgaattga acctggcctt cttcggggcc
     4321 tttcacggtc gcaccatggg ggccctctcc ctgacggcca gcaagaccat ccaaaagaaa
     4381 cactacaatc ccctggtgcc ggggatcacc cacgtgccgt atccgtactg ctaccgctgc
     4441 cctcacaacc tctgttatcc ctcctgcggg atcgcctgcg tcacgtggat cgaggacacc
     4501 ctttttcgaa ccacggtgcc tcccgaagag gtggcggcca tctttgtgga gcccatccag
     4561 ggggagggcg gctatatcgt gccgcccccg gaattccacc gggagctctt ccgcgtggcc
     4621 cgcaactacg ggattctcta cgtggccgac gaggtccagt cgggcatggg gcgcacggga
     4681 aagatgtttg ccatggaaca tttctcggtg gccccggaca tcctcgccct ggccaagggc
     4741 atcgcgtcgg gcctgcccct gggggcgacc ctggcccgct cccggatcat ggactgggaa
     4801 gcgggctccc acgcctcgac cttcgggggc aatcccgtct cctgccaggc cgccctggcc
     4861 accatcgagc tcctggaaaa cgggttgatg gaaaacgccg cccagcgcgg gcagcagctc
     4921 aaggcgggcc tcgaggagct ccaggaaatt tacgagccca tcggagacgt gcgggggctc
     4981 gggttgatgg tggggatgga actggtcaag gaccgggaga ccaaggagag ggcaggggaa
     5041 tggcgggacg cactgatcca ggccgccttc cgcaagggcc ttttgctcct cgggtgcggc
     5101 cagaacacca ttcgtttctg cccggccctg acggtgagcg cccgggaagt ttccgtctgc
     5161 ctcgagattc tcgagagcgt cttgcgggag ctcttttgac gaggatttcg aggcaccctt
     5221 gccgggaaga aaggggggca cacatcgaag cctcgatcag ggcattcggt tcgagtttga
     5281 atgacggggc aaaccccgaa gccgcagctg cagcaatagc cgcaggggtt cggggccggg
     5341 ggggggcgag agacgagagg gttggttagg tggttggctg gaggccagag gtttggcggc
     5401 caggaggtcg ggaggcgggg gagtggtgtg gcaggttgcg gaacaaggag aaaccatgaa
     5461 agggaaaagc gtcatcgcag gggttgtcgt cgtcgtgatt gccctggtgc tcttattgcc
     5521 ggctttgcct atctcctggt tcggagaaac ccgtggaagc ctcttcgcga agcccactcc
     5581 ggcggacacc gtgatccaca acggctccat tttgacttcg aacgaccgta gaccttacgc
     5641 cagggcactc gccatacgag acggcaagat cgtctatatc gggtccgaca acgggaaggc
     5701 tttgtccttt gtgggcaccg ggactcgggt gattgacgcg aagaagagcc tcgtcacccc
     5761 gggctttgtg gatgcacaca cgcacgctct ctggctgggc atcctttctc ccgtcatgac
     5821 cttcatctat gacgccggga gcctggagga cgttgccagg ctcgtgcgct cctatgggga
     5881 gcagaatccc gaccttcctt tcatcttcgc cataggctgg aaatacgact atatccccaa
     5941 cgggatccca acgctccgga tggcggacga catcctgcgg gaccggccgc tgattctttg
     6001 gagccacgac ggccatacgg gttgggtcaa cagcctggcc cttcaacaga tgcaggagag
     6061 aaaccccaag gccttcgagt acctgacgcc ggatttcgac aaggaccata agcccaccgg
     6121 gatcttccgg catttctaca ccatcaaccc catggatttc tacacggtgg aggaaatggg
     6181 cggagaggcc atcgttcgga agatggctca aggtgtggcg gccaagctcg aggaagggct
     6241 tcgcgtgggc gtcacttgcc atcacgacgt catgattcat cggagcacct tcgacctgct
     6301 gctcaagttg aagaagaggg gggttttcag ggatgcgagg gtacgcgcct ctcatttcgt
     6361 caatcatcat ctctacgagg acgacccggc cacactcttc ctcaacctgg cgagttggaa
     6421 acgacttgcg ggaagttcta cggaccattt tgtcatcggg gaatcgatca aactggggat
     6481 cgacggagtg tcgcccaacc acactgcgtt gctctataag ccctacaagg acgacaagac
     6541 caccaaaggc gtggcgagct acacggccga gggcttcaac cgactggtca ctcttatcga
     6601 ttccatgcgt ctgcagatat gcactcacgg ggtgggggac gccggcatca cgcggatcat
     6661 cgacggttat gagtttgcaa gaaaagtcaa cggcgcctgg gactcccggc ataccatcga
     6721 acacaattcc atgattgtcg acaaggacgt ccctcgcctg gcccggctgg gcatccacgc
     6781 atccatgcag cccactcatt atttcggcga ccgggccacg gtgaaggcct tgggaaaaag
     6841 gcgtttcctc cacgcccacc ggttcggaac gctgcagaga gcgaaagtgc ggctcgcctt
     6901 cggtaccgac tactcgatcg ttcccatgaa cccgatctac gggaccatcg tcgccgtgac
     6961 ccggttgagc tgtctcggtc ctcccaggac catggacgaa ttcaaggaga tgctctcctt
     7021 cgaagatgtc atccgtcatt acacactggg gagtgccgcg gcgctccaca tggaccgtct
     7081 catcggctcc ctcgaagtgg ggaagcgggc cgacctcgtg atcttctcca ttgatcggaa
     7141 cgaattttcc aggatgtgca atttgggaat catgccgccc tccgatatct tggaccaact
     7201 ggtatcgatg actttcgtag acggtcgggt ggtctatcag cgcccataga gcggaccccc
     7261 agattcggac cgggagtcgg ggagggaggc ggcaacccgg tggacctgcg gaagcggttc
     7321 gcgccggaac tcgaggcgac aagggtcgtc gtgcccttga agggagagaa atcgttctcg
     7381 gggccctcac cgcagtgggc gaggccgagg gtcggggaac ggcgtgaacc tcgagtaccg
     7441 cacccgcgag ggcgacagtg atcgacttcg accagcccga aaacaacgat tggccggcgg
     7501 tcaatctgtt cagcgtcacc gagaacaggc accgtcgccg gccggacgcg atgccgttcg
     7561 tgaacggtcc gtcgccggtg ctggagcaag cggaggggtt gtccgcggaa tgggcggcgg
     7621 cgtgcaaggc ggcgtccaac cgttgttggc ggcaccgaac cggagcgttg tgtgaagaga
     7681 agaaaagggg ggaggatgag cgaggatcac ccggagtggc tgttcgatga ggcgagacac
     7741 accggcgtcg actatgcgaa tcgggaggtg gttgcggact acgaccggca acatcagggg
     7801 ttcagggact tcgagaagga agccgagatg atcgtcgccg ctcttgactt gtcggagaac
     7861 tcgacggtct tggacgtcgg ctgcggcacc ggcgggctgt ccgtctgcct cgcacgcaaa
     7921 tgcaaacagg tctatgccgt cgatgtttca caagcgatga tcgaggtcct cgagggcaaa
     7981 atcgaagccc agggactggg caacatcaca ccccaacgag ccgggttcct gacctaccgg
     8041 cacagggcag aagcccccga tgccgttgtc gtcaacattg cccttcatca tctgccggat
     8101 ttttggaagc agattgccct ttgccgactc catgacctgc tcaagcccgg gggcaagatg
     8161 tttctggccg atgtcgtctt tggtttcgac ccgaggacct acgaggaagc cgtagaaggc
     8221 tggctgcact gcatgcggga cctcgcaggc ccgcggatgg ccgacgaaac gatcgtccac
     8281 atacgtgacg agttcagtac ctgggactgg gtgatgtcgg gaatgctgga acgtgccggg
     8341 ttccgcctcg aacagtgtct cgacctcatg ccccagatgc gagcctatat atgctcaaag
     8401 cccggagaac aggagccaag atgagcgatt cttcccgttg tcgggtgtgc ataaagcccg
     8461 cccccgggca atgccgcggc gggcttcgta caggcacggg agggtcgatt tcagccccgt
     8521 ggtacccggc ggtcggagaa agacctgtcg agggcccgaa aagttcggta acataggaga
     8581 aagggagccg aaaccgggtc gctttcgagg ataggcccat gacctgcaaa tgctttttcg
     8641 tggaacaggc ggacacccgg ggaggaccgg tgaccttcac cggggccacg gcccatcata
     8701 tggagcgggt ggctcggttg cgggttgggg acaccatcga gctgcgggac gggcagggaa
     8761 acgcctggct tgggtccatc gatgccatgg gccgaggggc ggtttcggtg cggatcctgg
     8821 gcgacgccga ggggcaccgg gagtctcccc tggaattgac cctggctatg gccttttccc
     8881 gggccgatcg catggactgg tccctgcgcc aggccgtgga aatcgggatc catcggttcg
     8941 tggctttccg ctcccggcgg agtcagtacg gcctgaacga gaaggaggcg gcggcgcggc
     9001 ggcagcgatg gtggaaaatt gctcgcgagg ccatgtgcca atgtggtaga gtgaagattc
     9061 cggaaatcaa tatcttacca gacctggacc gttacgtttc ccaaacggtc catgaggcag
     9121 cgggaccttc ggaagtcttg aagatcgtgg cctgtgaaga agggcctcga aggggccttt
     9181 tggacctgtg gtcacaaacg ccttggtgca agcaattcca tgcagtgatc ggaccggaag
     9241 ggggatggtc gccggaggac ctggaactgc tctttcgcgc agggttccaa tccgttcacc
     9301 tgggaccccg ggtcttgcgc atggagaccg cttcaaccgt cctgttggga gccgttcagc
     9361 tgttgtgggg agactttcac gtcgctcaag aaggggtata aacatgagat gtcacaagtg
     9421 tgggtaccat agtttcgact acctttccga gtgcaagatg tgcgggtcgg acttgagctc
     9481 gatccgggac tccctcggtc tcttgagcgt caagccggat atcccgtttt ttctgaacgt
     9541 gctgctccgc ggccctgggg cgggggaaac cgcattcccc actcccgagg acctggcagc
     9601 caccattgcg gaaatcgacc tcggtcccga gctggacctg gacgcacccc tggaggattt
     9661 tgccgggacc cttccccagg caccgccggg tcctgctccg gcacaggcgc agaccgaaga
     9721 gatcgagctc gtactcgagg acctgaagat tgccgaggaa gagcccatga tcgaactttc
     9781 ggacgcggac ttggaccagc tcagcgatcg ggagcagatg gagggattgg acctcaaagt
     9841 cgatctggac ctggatgcgg aacctcccct gaaaggactc tcccaggccg ctcccgggga
     9901 ggctgcgcgg gaggagcccg aggtcggaat cctgtc
//
LOCUS       DAOOVC010000142         9919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4336_length_9919_cov_5.949716, whole genome shotgun sequence.
ACCESSION   DAOOVC010000142 DAOOVC010000000
VERSION     DAOOVC010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9919
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4336_length_9919_cov_5.949716"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLM79_14585"
     CDS             <1..489
                     /locus_tag="PLM79_14585"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HOV87586.1"
                     /translation="IEVARSQIGVLEDPPGSNRGAQVEEYQRSVGIPPGEPWCAAFVY
                     WCFRRAAEQLGIANPVLRTGGVLDHWNRAYEEGIRRVTREQAMEDPSLALPGFIFIID
                     TGAPGGAGHTGLVEKVDGGMLVTIEGNTNEGGGREGIGVFRRSKRKILDINKGFIDYR
                     RA"
     gene            530..2101
                     /locus_tag="PLM79_14590"
     CDS             530..2101
                     /locus_tag="PLM79_14590"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOV87587.1"
                     /translation="MTQTPKTFQKPVKPRGSQVLDSLKSRISPGLPYGEAFLRFRELF
                     LVVGERGLGLAVNLLVLVLVERSHGQAGLGVFSYLWSFYHLTAYVAEWGMSPYVERET
                     ALHYGYAGKQQEVLRDGFQQVFLMALLAGVLCIASAAYDTTHTRIEEKMGAYFLIGLA
                     VPLHNLNKLRLAFLNGQGRHEEAARLQGRKRLVFLGTSFLLMATGLQPSYLVAAFPVS
                     ELILARAGRKRQRLPALRSVWVGVEPLKKTLSQGSRLLLTDDALDVVLYLDLLILGFF
                     VPAWDLGVYAEASILARFFLLIPTSIRPVFRRRFCVLASRSEHLQAAALFHKAALHLF
                     ALHSFVILLLLLFYPEILRFFFWTRGEELSSFRVFGILAPGLLFSASVLACEPLYEAL
                     GRTDSLRNLMMAVFGTTVFLNLTLVPMAGLQGAAWATTLALVLHFLLFTWFLEAPYRW
                     KMNLYLVAGGAVYVTYTVLEALEVGAAPTLVLLPVLLGVQFYGIGFFSEDPGTLGRSN
                     DGGTVHATAGEEEFS"
     gene            2070..3158
                     /locus_tag="PLM79_14595"
     CDS             2070..3158
                     /locus_tag="PLM79_14595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOV87588.1"
                     /translation="MQQPEKKSFLNLGRLRERFRIPPFPWTGKTRVLIAVLALATILY
                     RLMLVYIEPGEFGIKVVRLGANRGVQKQVHGAGLHVVIPGIQQLYRLPMGIQVLELTN
                     YPTTAAYRARQEKAAHIQTSDGFFVDVDVSILYRLVDPYLVFTLIGPGTLYEDNGIIP
                     KAEPILKATLGELTTEEFYNSPLRVKRCEAAKEILNRELNPKGIKVERVMVRYFRYSD
                     EIQKNIEEKKLKDQLVFKNQAEARAATEEATLKKIVQEGEAVLSVKIEEGKAYVTRKR
                     AEKDLYVRTRTAEADLMVKLAEAERVRLKNEALEGEGAERMVGLKMADVYKGLQTVIL
                     PSDGPDGVNPLSLEKTLQLFDVRKGGGK"
     gene            3155..4189
                     /locus_tag="PLM79_14600"
     CDS             3155..4189
                     /locus_tag="PLM79_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HOV87589.1"
                     /translation="MRRILPVLVLGFLLSVPGGCARTTGETEVGVRTVKIGLFAPKGV
                     ENRVYAPGSTYIFLPFVNDWHTFDTKLQNLEMTIHSSRGDRKSRDDLLFKTIDGNDIG
                     LDVIIAYRIDPSQAPYILQYVAKDNRTLREKVVRTVARSKPRDIFGELKTEEFYVADT
                     REAKAQAAKEVLQEILGTMGIVVERVFTKDYRFNPEYQKAIEDKKVADQRVEKNRSAQ
                     HAATEEYKRKLEDAKGEVNKMVASVDGEFRMAQIEVDAYFERQSLLAKAIRAEGIAEA
                     AGIREMNKALLGTGGEALVKLRIAEALQDKKIVLLPVSEGGMNLKTLDMNRLIETLGI
                     RSLAAPAEKP"
     gene            4920..6524
                     /locus_tag="PLM79_14605"
     CDS             4920..6524
                     /locus_tag="PLM79_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HOV87590.1"
                     /translation="MSDQTIQITFLAAGILVLAWMGVLAQRRVKAHEDMSVGGRSFYS
                     WSIFFSLFAFWGGNTIAGIVELAHKDGIASAWFGIARMTMFLLILFITGGAFRRLAMI
                     TLSDFIARRFGSPFLRLLAGIIIAMNFTIFTVSSVVGAAAFFTAILGWSVWTSVLFTV
                     VSFLTYTTLGGMHAISYNGKILVVGQLLALLAAACLGVKMAGWENILKLEPRYFQVLP
                     PTYPSTVLMWFFTFVVNAFVAQAALQIVMSCKTVQEGRRGILYVTLGFIPIVILSPLA
                     GMAAKVLYPEIPSVQAMPMLASNMPSSVLSAVVVLGLYFTTLGWASSCILSGGTVAAH
                     DIYRYFVPNATSAQLIRASRISIFLLSLLTVGFAILLPSGVEFWTIVGFVLRNTALFP
                     LVLVGLFWNMISRRAAIGAALAGGVTGVVWYLIRFPAYLWDAHPMFVGMVVSMVVLLG
                     GTLVEYRGRIGVRPSPKGSVFGLLGMAGVGLTLVYGAKLLKMKCLFAAVGFDVSFLFA
                     AVIFYIRQVHGDGTVVELPLEVEECS"
     gene            6556..7506
                     /locus_tag="PLM79_14610"
     CDS             6556..7506
                     /locus_tag="PLM79_14610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOV87591.1"
                     /translation="MGRESKWASGRLKKIRGMVSAGAGALLLLFPAGGFAGIFDAGSW
                     GYQLQDAVPSELAASAYDVLVIDYSKDGSDSGAYSRRAVESIRRKGKIVLAYFSIGEA
                     EDYRFYWNDRWRPGSPPWLGPENPDWKGNYKVRYWESGWWNVALRPYLDRILDAGFDG
                     AYLDIVDAYWYWRKEPGFTVGIAASKMIRLVEKIGNYARQTFPDFILCPQNAESILDD
                     VPTAKLRKRYLDAIQAIGVEDLFYHYGSSADREYRKAMLKQFAAAGKRIFNVEYVSGD
                     RWKAYLNRACSAPFPVVPYGARPDRELDELVTFPKKPCGP"
     gene            7781..8035
                     /locus_tag="PLM79_14615"
     CDS             7781..8035
                     /locus_tag="PLM79_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87592.1"
                     /translation="MRILFRSPDKNRDGRAILQEYSVIRKKSDKAERSFPHFDRNSDG
                     VLAQEELTRMSDELERLLDAASPQPEAPVWPKTGKGQGGR"
     gene            8069..8326
                     /locus_tag="PLM79_14620"
     CDS             8069..8326
                     /locus_tag="PLM79_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87593.1"
                     /translation="MPYIPTERRTVFDSHLEACAREVRNGGELNYCIYKLASLLIRRI
                     GESYQNLSMCSGAMEHAKLEWVRRRLSPYEDRKILENGDID"
     gene            8508..9416
                     /locus_tag="PLM79_14625"
     CDS             8508..9416
                     /locus_tag="PLM79_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOV87594.1"
                     /translation="MKWLLGFLALCCSAALFPFGGIARAAGTVPVAVSIPPQKYFVEK
                     IGGRWVAVSVMVEPGANPHNYEPKPQQMIQLAAAKIYFAVGDPFEKVWLRKFAAANPA
                     MLIVNTEQGIRKLPMDSHGHGEEAPRHGKSGKKEAIPEGLDPHVWLSPPLVMLQARNI
                     LDAFLRVDPEHRSDYEANYARWVTEIVQLDLYLLEKLSRRDVPREFMVFHPSWGYFAG
                     AYGLKQLSVEVEGKEPKAAELKELIRHAKERNIRSVFVQPQFSGATAKVIAEAVGGRV
                     VMADPLAEDWDRNLREMGDKLSAALP"
     gene            9431..9901
                     /locus_tag="PLM79_14630"
     CDS             9431..9901
                     /locus_tag="PLM79_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOV87595.1"
                     /translation="MCVRCDYSASLESVDLPVTPHRLLVLEVVGNSSYPLSPQEIFET
                     VRRSHPMNRVTLYRILDLLVEKELVERISSGDRTFRYGPAPSANHPPHPHFFCTRCRS
                     MECLQPEALPLDVRSLQRTFPALIEKVEIRLDGICSNCLRRKKARSMRPSSRRP"
BASE COUNT         1835 a         2931 c         3132 g         2021 t
ORIGIN      
        1 atcgaggttg ctcgatccca gatcggggtc ctcgaggacc cgccggggtc gaaccgggga
       61 gcccaggtcg aggaatacca gcgaagtgtg ggcattccac ccggagaacc ctggtgtgcc
      121 gcctttgttt actggtgctt ccgccgggcg gcggagcagt tgggaatcgc caatcccgtc
      181 ctccgcaccg gaggcgtcct ggaccactgg aaccgggcct atgaagaagg catccgccgg
      241 gtgacccggg aacaggccat ggaagatccg tccctggctc ttcccgggtt catcttcatc
      301 atcgacacgg gggcgccggg cggggcaggg cacacgggcc tcgtcgaaaa ggtggacggt
      361 ggcatgttgg tgaccatcga aggcaatacc aacgaaggcg gcggccggga aggaatcgga
      421 gtatttcggc gcagcaagag aaagattctg gatattaaca agggattcat cgattatagg
      481 agagcgtgat ccgacgaaat gagggcccgg aacccccgga ggcgagaagg tgacccagac
      541 tccaaagact ttccagaagc ctgtgaaacc tcgcggttcc caggtcctgg acagcctgaa
      601 aagccgcata tccccgggcc tgccttacgg ggaagccttt ctccggttcc gggaactctt
      661 cctggtggtg ggagaaagag ggctgggact ggccgtgaac ctcctggtgc tggtcctggt
      721 ggaacggagt cacggtcagg cggggctggg cgtcttttcc tatctctggt ccttctatca
      781 cctcacggcc tatgtggctg agtggggaat gtctccctac gtggaaaggg agacggctct
      841 tcactacggc tatgcaggca aacagcagga agtcctgcgc gacggtttcc agcaggtctt
      901 cctcatggcc cttcttgccg gggtcctgtg cattgcctcg gcggcctacg acacgaccca
      961 cacccgcatc gaagaaaaga tgggggccta tttcctcatc ggtcttgccg ttcccctgca
     1021 caacctgaac aagttgcgcc tggcctttct caacggccag gggcgccacg aggaggcggc
     1081 ccggcttcag gggagaaagc ggctggtatt cctggggact tcctttttgc tgatggcgac
     1141 gggccttcag ccgtcgtacc tggtggcggc tttcccggtg agcgagctga tcctggcccg
     1201 ggccgggaga aagaggcaga gacttccggc gcttcgctcg gtgtgggtgg gcgtcgagcc
     1261 cctgaagaag accctaagcc agggctcccg gctcctcctg accgacgacg ccctggacgt
     1321 ggtgctctac ctggacctgc tgatcctggg attcttcgtg cccgcatggg acctgggggt
     1381 gtatgccgag gcctcgatcc tcgcccgctt ctttctcctg attcccacga gcatccgtcc
     1441 cgtcttccgc cggagattct gcgtcctggc ttctcgatcc gagcatttgc aggccgcagc
     1501 gttgttccac aaggcggccc ttcatctctt tgccctgcat tcctttgtga ttctcctttt
     1561 gctcctgttc tacccggaga ttcttcggtt cttcttttgg acccggggag aggagctttc
     1621 ctccttccgc gtcttcggaa ttctggctcc gggtcttctt ttttccgctt ccgttctggc
     1681 ctgcgagccc ctctacgaag ccctgggccg cacggattcc ttgcgcaacc tcatgatggc
     1741 ggtcttcggg acaacggttt tcttgaacct gaccctggtt cccatggcgg gcctgcaggg
     1801 ggcggcctgg gcgaccaccc tggccctggt gctgcacttt ctgttgttca cgtggttcct
     1861 ggaggccccg taccggtgga aaatgaacct gtacctggtg gccggaggcg cggtatatgt
     1921 gacgtacacg gtcctggaag ccctcgaggt cggggcggcc cctaccctgg tgctgctgcc
     1981 cgtgctgttg ggcgttcagt tttacgggat cggatttttc tcggaagatc cagggaccct
     2041 gggtcgttct aacgatggag gaaccgtcca tgcaacagcc ggagaagaag agttttctta
     2101 acctggggag gctccgggag cggttcagga ttcccccttt cccctggacg ggaaagaccc
     2161 gggtcctgat cgccgtcctg gcgctggcaa caatcctgta ccggctcatg ctggtctaca
     2221 tcgagcccgg ggagttcggg atcaaggtgg tgcggctggg ggccaatcgc ggcgttcaga
     2281 agcaggtaca cggagcgggc ctccacgtgg tcatcccggg gatccagcag ttgtaccggc
     2341 tgcccatggg gatccaggtc ctcgaactga ccaactaccc caccacggcg gcctaccgtg
     2401 cccgacagga aaaggcggcc cacatccaga cctccgacgg gtttttcgta gacgtggacg
     2461 tgtccatcct ctatcgcctg gtggatccgt acctggtctt caccctcatc ggccccggga
     2521 ccctctacga ggacaacggc atcattccca aggccgagcc catcctcaag gcgaccctgg
     2581 gggaactcac caccgaagag ttctacaaca gcccccttcg ggtcaaaagg tgcgaagccg
     2641 ccaaggagat cctcaatcgg gagctgaacc ccaagggaat caaggtggag cgcgtcatgg
     2701 ttcgttactt ccggtatagc gacgaaatcc agaagaacat cgaagaaaag aaactcaagg
     2761 accagctcgt gttcaagaac caggcggaag cccgtgcggc caccgaggaa gcgaccttga
     2821 agaagattgt ccaggagggg gaggccgtcc tctcggtgaa gatcgaggaa ggcaaagctt
     2881 acgtcacccg caaaagggcc gaaaaggacc tctacgtccg gacccggacg gcggaggcgg
     2941 acctgatggt gaagctggcc gaggccgagc gggttcggct caagaacgaa gccctcgagg
     3001 gggaaggggc cgagcgcatg gtgggactga agatggccga cgtatacaag ggattgcaga
     3061 cggtgatcct gcccagcgac ggtcccgacg gggtcaatcc cctgagcctc gagaagaccc
     3121 tgcagctgtt cgacgtgcgg aagggaggtg ggaaatgaga agaatcctgc ctgtgttggt
     3181 tctgggtttt ctcttgtcgg tccccggagg gtgtgcccgc accaccgggg agaccgaggt
     3241 gggggtcaga acggtgaaga tcgggctgtt tgctcccaag ggggtcgaga accgggtcta
     3301 tgccccgggt tccacctaca tcttcctgcc cttcgtcaac gattggcaca ccttcgacac
     3361 caagctgcaa aacctcgaaa tgacgatcca ttccagccgc ggggaccgaa agtcccggga
     3421 cgacctgctc ttcaaaacca tcgacggcaa cgacatcggc ctggacgtca tcatcgccta
     3481 ccggatcgat cccagccagg cgccctacat tctccagtac gtggccaagg acaaccggac
     3541 cctccgggaa aaggtcgtga gaacggtggc gagaagcaag ccccgggaca tcttcgggga
     3601 actcaagacc gaggagtttt acgtggcgga cacccgggag gcaaaggccc aggccgccaa
     3661 ggaggtgctc caggagatcc tcgggaccat ggggatcgtg gtggaacggg tcttcaccaa
     3721 ggattaccgt ttcaatcccg aatatcaaaa ggccatcgaa gacaagaagg tcgccgacca
     3781 gcgagtggaa aagaaccgct cggcccagca tgcggccact gaggaataca agcgaaagct
     3841 cgaggacgcc aagggggaag tgaacaaaat ggtggccagc gtggacgggg agttccgcat
     3901 ggcccagatt gaagtcgacg cctatttcga gcggcagtct ctcttggcca aggccatccg
     3961 ggcggaagga atcgccgagg cggcggggat ccgggagatg aacaaggcgt tgttgggaac
     4021 gggcggcgag gcgttggtga agctgaggat cgccgaagcc ctccaggaca agaaaatcgt
     4081 cctgttgccc gtttccgaag ggggcatgaa cctcaagacc ctggacatga atcggctcat
     4141 cgaaaccctg ggaattcgat ccctggccgc ccccgccgag aagccctagc cgaggcggag
     4201 gggtcgggtc tccgtatgag gcggaggggt cgggtctccg tatggctgac ctcaggggtc
     4261 aggtctctgc acggcttgtc ccgtgggtgc ggaggatgta ggggcgaccg gccggccacc
     4321 cctaccgggc gtgcggggcg caaccccact cttgttcacg ggacggtcct gcgaccccat
     4381 gggtgggctt tctcttggga gctgttccgg gggtatcggc cctgcggcct cgagtcccgg
     4441 cgaatggttg cgaacccgcc gggtgcgctc ggaggcggcg atccacccct gcccgatggc
     4501 agatgtgggt aaagggcggc cccagccgag gcggggtgct cccgggggtc cgacccgggt
     4561 tactcacatc ccgggcatcc gagactgatc cgggatactg aattcgtctt cctttccaat
     4621 cctcacccgc cgcggaagag gaggttttct ccatcctgta gcgggatgtt cgcccgtctt
     4681 cccacccctg ccgccgtctt acggctcttt ggtctccgct ctcttcctcc ccttttcggg
     4741 cgacttcgtt gaaatatttc acaggaaggt gcatgctagg cgccgttatg ggaaggatgg
     4801 gtccgctttg cggaccgttc gacgcattcc gcggccgtgg agagaggagt gcgttcaggg
     4861 ccggggaggg tcccgggcga cgtttacggc cgaaaaagga aatggagcgg cacggggcaa
     4921 tgtccgatca aaccattcaa atcacgtttc ttgcggcggg aatcctggtc ctggcctgga
     4981 tgggggtcct cgcccaaagg agggtcaaag cccacgaaga catgtccgtg ggagggcgca
     5041 gcttttattc gtggagcatc ttcttctcgc tttttgcctt ctggggcggg aataccatcg
     5101 ccgggatcgt cgaactggcc cacaaggacg gcatcgcgtc ggcctggttc gggatcgccc
     5161 gcatgaccat gttcctcctg atcctgttca tcacgggagg agcctttcgc aggctggcca
     5221 tgatcaccct gtccgacttc atcgcccggc gctttggatc cccgtttctg cggttgcttg
     5281 cgggaatcat catcgccatg aatttcacca tctttacggt gagttccgtg gttggagccg
     5341 cggccttctt tacggccatc ctgggatggt ccgtctggac gtcggtcctc tttaccgtgg
     5401 tttccttcct tacctacacg accctggggg gaatgcatgc catttcctac aacgggaaga
     5461 tccttgtggt gggccagttg ctggccctcc tggcggcggc gtgcctgggt gtgaagatgg
     5521 ccgggtggga aaacatcctg aaactggaac cgcggtattt tcaggtcctg cccccgacct
     5581 acccgtccac ggtgctcatg tggttcttta ccttcgtggt caacgccttc gtggcccagg
     5641 cggccttgca gatcgtgatg tcgtgcaaga cggttcagga agggcgaaga ggaatcctgt
     5701 acgtcaccct cgggttcatc cccatcgtga ttctgtcccc cctggcaggc atggcggcca
     5761 aggtcctcta tccggaaatc ccctccgtcc aggccatgcc catgttggcc tccaacatgc
     5821 cttcttccgt cttgtccgcc gtggtggtgc tgggtcttta cttcaccacc ctcgggtggg
     5881 cgtcctcgtg catcctttcg gggggcaccg ttgcggccca cgacatctac cgctatttcg
     5941 tgcccaacgc cacctcggcc caattgattc gagcctcgag gatctccatc tttctccttt
     6001 cccttctcac cgtggggttc gccatcctcc tccccagcgg cgtcgagttc tggaccattg
     6061 tggggttcgt tttgagaaac acggcccttt tccccctggt cctggtgggg ctcttctgga
     6121 acatgatttc ccgcagagcg gccattgggg ccgctttggc cggaggggtg acgggagttg
     6181 tctggtatct catccgattt cccgcgtatc tctgggatgc tcatcccatg ttcgtgggca
     6241 tggtggtttc catggtcgtc ctcctggggg gaacccttgt ggagtaccgg gggcggatcg
     6301 gggtccgacc cagcccaaag ggatcggtgt ttggcctgct gggcatggcg ggagtcgggc
     6361 tgaccttggt gtatggcgcc aagctcctga agatgaagtg cctctttgcc gccgtgggtt
     6421 tcgacgtctc gtttcttttt gcagcggtga ttttctacat ccggcaagtc catggggacg
     6481 gtaccgtggt ggaattgccc ctcgaagtgg aggaatgcag ctgagccttc ggccaccata
     6541 agacaggagg cagatatggg ccgcgagtcc aagtgggcat cgggacgttt gaagaaaatc
     6601 cgggggatgg tgtcggcggg agccggggcc ttactcctgc tctttcccgc gggaggcttt
     6661 gccgggatct ttgatgccgg aagttggggg tatcagcttc aagacgcggt gccctcggaa
     6721 ctggccgcct ccgcctacga cgtgctggtc atcgattatt ccaaggacgg gagcgattcg
     6781 ggcgcctact cgagacgggc cgtggaatcc attcgcagga agggaaagat cgttttggcc
     6841 tatttcagca ttggggaggc cgaagactac cggttctact ggaacgaccg gtggcggccc
     6901 ggcagtcccc cctggctggg tcccgaaaac cccgattgga aagggaacta caaggttcgc
     6961 tactgggaat ccgggtggtg gaacgtcgcc ctgcggcctt acctggatcg gattctcgat
     7021 gcgggcttcg acggagccta cctggacatc gtggacgcct actggtactg gcgaaaggaa
     7081 ccgggcttca cggtggggat cgcggcatcc aaaatgatcc ggctggtcga gaagatcggc
     7141 aactatgccc ggcagacctt tccggatttt atcctttgcc ctcagaacgc cgagtccatc
     7201 ctggacgacg ttcccaccgc caagcttcgc aaacgctacc ttgacgccat ccaggccatc
     7261 ggggtggagg atcttttcta ccactacggt tcttccgccg accgggaata ccgcaaagcc
     7321 atgctcaaac aatttgccgc tgcggggaag aggatcttca acgtggaata tgtttccggc
     7381 gaccgatgga aagcgtatct caaccgggct tgcagcgcgc ccttcccggt ggttccctac
     7441 ggggcaaggc cggatcggga actagatgaa ctggtcacct tcccgaaaaa gccttgcggg
     7501 ccatgagcca tcgaggggat ctcgcaagcc ttggggtcgg tcgtgcccgc cgccttccgg
     7561 aaaggcggcc ccgggggacc gaacggcaga ttcgaggagc ggacgttccc gggaggacgg
     7621 ctcatgcccc gtccccgtgg gttctccgat ctcgaaaacg ggccttcagg ggccggggag
     7681 acctcatgaa aacgactttt tggatggttg tcctttgggt cgtcccgggg aagtccgggt
     7741 ccgttcgcgt cggcgcccgg ccctccccgg aggcaacgaa gtgaggattc tctttcgcag
     7801 cccggacaag aaccgggacg ggagggccat cctgcaggag tattccgtca tccggaagaa
     7861 gtccgacaag gccgaaagga gttttccgca tttcgatcga aactccgacg gggtgctcgc
     7921 ccaagaggaa ttgaccagga tgtccgatga gctcgaaaga ctgttggatg cggcttcccc
     7981 ccagccggag gccccggtgt ggcccaagac ggggaaggga caaggaggcc gttgatttca
     8041 cggatccccg gaagaaagat gcaaagccat gccctacatt cctacggagc gaagaacggt
     8101 ttttgattcc cacctcgagg cttgtgcccg ggaggtccgg aacggcggcg agctgaacta
     8161 ctgcatctac aagctggcaa gcctcctgat ccgcaggatc ggagagagct accagaacct
     8221 cagtatgtgt tcgggagcca tggagcacgc caagctcgaa tgggtccgca gaaggctctc
     8281 cccttacgaa gaccgcaaaa tcctggaaaa cggggatatc gattagggtt tccttccccg
     8341 ggcggttggt ccttcgatcc ttacttttcc gggttctcca tgcaacttgg ttgcattctt
     8401 cctgtatttc ctttattctt taaaaaaaac cgtgtcgcgg gttttcaggg ccgtgcaagt
     8461 cgtttgatta cggcccatcc tggggaagat aaggaggtct tcggtccatg aaatggttgc
     8521 tgggtttttt ggcattgtgc tgcagcgctg cgctctttcc cttcgggggg attgcccgtg
     8581 ccgccgggac cgtccccgtg gcggtcagca tcccgcccca aaagtacttc gtggaaaaga
     8641 tcggaggccg gtgggtcgcg gtttcggtca tggtcgagcc gggggccaat ccccacaact
     8701 atgaacccaa gccccagcag atgattcagc tggctgccgc gaagatctac tttgccgtgg
     8761 gagatccctt cgagaaggtt tggctcagga agtttgcggc cgccaatcct gccatgctca
     8821 tcgtgaacac cgaacaaggg atccggaagc ttcccatgga ttcgcacggc cacggggaag
     8881 aggccccccg ccacgggaag tcggggaaga aggaagcgat ccccgagggc ctcgatcccc
     8941 acgtgtggct ttcccctccc ttggtgatgc tccaggcccg caacatcctg gacgccttcc
     9001 tccgggtgga tcccgagcac cggtcggact atgaagccaa ctacgcccga tgggtgacgg
     9061 aaatcgttca actggatctg tacctcctgg aaaaactctc ccggcgggac gtgccccggg
     9121 aattcatggt gttccacccg tcttggggat acttcgccgg ggcctatggg ttgaagcagc
     9181 tttcggtgga agtggagggc aaggagccca aggcggccga actcaaggag ctgattcgcc
     9241 acgccaagga gcgaaacatt cggtccgtgt ttgtccagcc tcagttctcc ggcgccactg
     9301 ccaaggtgat tgcggaagcc gtggggggcc gggtggtgat ggcggacccc ctggccgaag
     9361 actgggaccg gaacctgagg gaaatgggag ataagctgtc ggcagctctc ccgtaggagg
     9421 aggcgaaggc atgtgtgtgc ggtgcgatta ttcggccagc cttgaaagtg tcgatctgcc
     9481 cgtcactcct caccggctcc tggtcctcga ggtcgtcggc aacagctcgt atcccctcag
     9541 tccccaggaa atcttcgaaa ccgtgcggcg gtctcatccc atgaaccggg tgaccttgta
     9601 ccgcatcctg gacctgctgg tggaaaagga gctggtggag cggatcagct cgggagaccg
     9661 gacctttcgt tacggacctg ctcccagcgc caaccatccc ccccatcccc acttcttttg
     9721 tactcgatgc cggagtatgg aatgcttgca gcccgaggcg cttcccctgg atgttcgttc
     9781 cctccagagg acctttccgg ccctcattga gaaggtggaa atccgcctgg acggaatctg
     9841 cagcaactgc ctgaggagaa agaaagcccg gagtatgcgc ccgtcctccc gccgcccgtg
     9901 agggagacgc ggggtctcc
//
LOCUS       DAOOVC010000143         9913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4338_length_9913_cov_6.149117, whole genome shotgun sequence.
ACCESSION   DAOOVC010000143 DAOOVC010000000
VERSION     DAOOVC010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9913
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4338_length_9913_cov_6.149117"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLM79_14635"
     CDS             complement(<1..551)
                     /locus_tag="PLM79_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87596.1"
                     /translation="MKKWVLSGLVALLLLLPFRVLAVEEAVSIDLPGASEVSPAIKAY
                     KLIGIPVLPPSSTDVFEVLKGFFGSQADPGVWRLYAYVGGRYVEITGLDQDSVDYGKG
                     WWIISAQAKTLNISGLPFPSFEWSGSMPAGYSILANPFHDTAIVWQDVVDYLSNSPLG
                     LGAYIYKWSDVLGEYVIVTEMLPG"
     gene            complement(732..1433)
                     /locus_tag="PLM79_14640"
     CDS             complement(732..1433)
                     /locus_tag="PLM79_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOV87597.1"
                     /translation="MKSLWVLYLATLTCLIWNLAAAPENGWAAGEKTDPSGVVKLPQP
                     RFQGDVSVEAALARRRSVRDYKREPLALPDVGQLLWAAQGVTHGAGLRTAPSAGALYP
                     LELYVVSGEVEGLPPGIYKYRPASHDIARTAVGDKRAALCSVSLSQWAVKNAPAVVVI
                     TAVPERTTRKYWSRGTRYVHMEAGHAAENLCLQAVALDLGSVIIGAFQDDDVKRVLDL
                     PEEEQPMLIIPVGKR"
     gene            1745..1820
                     /locus_tag="PLM79_14645"
     tRNA            1745..1820
                     /locus_tag="PLM79_14645"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1778..1780,aa:Lys,seq:ttt)
     gene            1933..2010
                     /locus_tag="PLM79_14650"
     tRNA            1933..2010
                     /locus_tag="PLM79_14650"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1969..1971,aa:Glu,seq:ttc)
     gene            complement(1989..2237)
                     /locus_tag="PLM79_14655"
     CDS             complement(1989..2237)
                     /locus_tag="PLM79_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87598.1"
                     /translation="MKPTGDNLPPMGNRFNTEIFHGSARTRFLTIPLRRNTMREKEKT
                     FREDARKAWLYPPLYPGTVLKVFQNSTSLESFGVPNRI"
     gene            complement(3279..3839)
                     /locus_tag="PLM79_14660"
     CDS             complement(3279..3839)
                     /locus_tag="PLM79_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87599.1"
                     /translation="MSLKTGSSANCVPFGLTMDDHSVAVSFFRYRLRKRKPPRKLPLF
                     PLVLFLGLVLPSFAGVNSEEELRKQVPPAHKVTITAYTNVPECTDSTPNETASLLQIR
                     SRHYGKIIALSRDLAQHYRFGDKFQLWIDGKSQVVEYQDLMAARHANRIDLLLPNLRK
                     CRGFGAKDGILVPLGKAGKRSNERTG"
     gene            4094..5644
                     /locus_tag="PLM79_14665"
     CDS             4094..5644
                     /locus_tag="PLM79_14665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87600.1"
                     /translation="MRIRTCREREPSMRGGLAAGSPYDKETAVTVYDYDIGVIGGGAA
                     GLTVAAGTAQLGAGTLLIDREPRLGGDCLHYGCVPSKTLIRTAHVYHLMRRAAEFGLP
                     SVDLPPPDFLEIARRIRSVIGEIQRHDSVERFCKLGAKVEFGEATFADEHSIRLNGSL
                     PSARHWVVATGSSPAIPAIKGLDETPYLTNREIFSLDRLPRSLIILGGGPIGIEMAQA
                     FARLGTDVRVVESGPRILPRDDRDLTDRVMEVLASEGVRFCLSSRAVEVRNPGGERQV
                     VLEDPNGQRTVVRAEELLVAAGRVANIRGLGLESLGIKFDVKGISVDSRLRTARKHIY
                     AAGDVTGLYQFTHAAGYEGGIVVANTVFRLPRKTDYRRLPWCTYTDPELASIGMNEGM
                     ASRAGIEYSVWTEEFEANDRSLAEGARDGKIKLILDAREKALGIQILGPRAGELLGEW
                     VAALNGQVKLTTLASSIHPYPTLGEINKKVVSSYLSPKIFSERIRKGLRLFFNLKGRA
                     CGTETTSS"
     gene            5726..6640
                     /locus_tag="PLM79_14670"
     CDS             5726..6640
                     /locus_tag="PLM79_14670"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOV87601.1"
                     /translation="MDIAQLTGLIQLLMDLAQKVARGEYDRADELLELTRQDRYPAMI
                     RDLAESFGMMLVQVEAREFHLEQMIDELAAKNRQIEATLKKVRLLENIRDQLSKFVPS
                     SVRNLIEQNPENPDLEKRDEDVTVLFLDIAGYTRLSEQVEPEKVNYLIQTYFSSFLDV
                     IFENEGDINETAGDGLMILFRDPDGRAHAANAVRAAVGIRDRAGAINEAHRGLYAPVI
                     VNMGINSGPASVGSTRFEGVSGTRWTFTASGPMTNKAARISSLAADGAILTGHETYRR
                     VAKTFPLEPLGRHRLKGIQKPILVYRVL"
     gene            complement(6779..7225)
                     /locus_tag="PLM79_14675"
     CDS             complement(6779..7225)
                     /locus_tag="PLM79_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV87602.1"
                     /translation="MAYKTIVCGVTGSIPSRRAALEAAVLAGEAQADLVYVYAVDVEF
                     LKTGKAVQLGSEFAEESLAHLGGHMLDFADEIAPSQGVTPKKVVRKGPVLDVLKQVVL
                     EEEADLLVLGHEDRTFFEKILFKGSVEDHVQELESQTGVEVRIVRG"
     gene            complement(7306..8673)
                     /gene="nhaA"
                     /locus_tag="PLM79_14680"
     CDS             complement(7306..8673)
                     /gene="nhaA"
                     /locus_tag="PLM79_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOV87603.1"
                     /translation="MKENVYILEPLFGRMLSPFEQFLRRATAGGIILMSVTVATLLLA
                     NSPWGQFLHDFWEQRAGIGIGGFRVEMTLHHWVNEGLMALFFLLVGLELKREVLVGEL
                     SSLREAVLPVVAAVGGMVVPAILYILVNRDGVEAKGWGIPMATDIAFSLGILVLLSWR
                     IPKSLIVFLAALAIADDLGAVLLIALYYSTSLDIGALKGALAILAVLALLNRGGIRHP
                     LPYGVMGMALWLVLLHSGIHATVAGVLLAFFIPARPVFTPVEFEQRLDHLQETLRYEV
                     EDPEACLQPLKCPVMATVAQNVEKSAKAVQSPLQRMEHHLSPWVTFVVIPLFALSNAG
                     VNFSNMEVSRVLGQPVTWGIVLGLVLGKFAGVSGFSWFAVKCGFARLPSGVCWHHLVG
                     VAWLAGIGFTMSLFINELAFDDPGMAIQAKVGILLASLLAGTIGVAWLLAVASAGKDD
                     DPSPP"
     gene            complement(9703..>9913)
                     /locus_tag="PLM79_14685"
     CDS             complement(9703..>9913)
                     /locus_tag="PLM79_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87604.1"
                     /translation="AGKYVILNLTGTQLHKDGWRDTGIMQIQLNTSTSPMKGTFYEVG
                     TDYDLNSKTYSNHFSRGTLKRINCP"
BASE COUNT         2044 a         3034 c         2950 g         1885 t
ORIGIN      
        1 cccggcagca tctcggtgac gatgacgtat tcccccagga catcggacca cttgtaaatg
       61 taggccccca atcccagggg actgttggac agataatcca ccacgtcctg ccacacaatg
      121 gccgtatcgt ggaaggggtt cgccaggatg gaatatccgg cgggcatgga accggaccat
      181 tcaaaagaag gaaacggaag cccggaaatg ttcagggtct tggcctgggc ggagatgatc
      241 caccacccct tgccgtaatc gacggagtcc tggtccaggc cggtgatctc cacgtacctt
      301 ccgccgacat aggcgtagag tctccacacg ccgggatccg cctgacttcc gaagaaaccc
      361 ttgagcactt cgaacacatc ggtactggat ggcggcagga cggggatgcc gatgagcttg
      421 tacgccttga tggccgggct cacctcggac gctcccggca ggtcgatgga aacggcctcc
      481 tccacggcca acacccggaa aggaagcaac agcaacagag cgacgagtcc tgacagtacc
      541 cactttttca cggcagcccc tccattcggg atgttcgatg cgatcgggca acgatacccg
      601 gatatccttc agcggacctt ccgcattcgt ccagcccatt ttcccgtttc catccggttc
      661 ggccccggcc gaacccggaa gcggcccggt cccccggccc gggtaccgaa aggaatcgcg
      721 aggaatcgat cttatctctt gccgaccggt atgatgagca tgggctgttc ttcttcggga
      781 aggtccagca cccgcttgac gtcgtcgtcc tggaaggccc cgataatcac cgacccaagg
      841 tcgagggcca cggcctggag acagaggttt tcggcggcgt gtcccgcctc catgtgcacg
      901 tagcgggtcc ctcgagacca gtatttccga gtggttcgtt ccggcacggc ggtgatcacc
      961 accacggcgg gagcgttctt gaccgcccat tggctcaggc tcacgctgca gagcgccgca
     1021 cgtttgtccc caaccgccgt ccgggcgatg tcgtgggaag cgggcctgta cttgtaaatg
     1081 ccggggggca acccctcgac ttccccgctc accacgtaga gttccagggg ataaagtgcc
     1141 ccggccgaag gcgccgtgcg cagccccgcc ccatgggtca ctccctgggc cgcccacagc
     1201 agttgcccca cgtccggaag agcgaggggt tctctcttgt agtctcgaac ggatcgcctc
     1261 cgggccaaag ccgcctccac ggaaacgtcc ccttgaaaac ggggttgggg aagcttgacg
     1321 acgcccgacg gatcggtctt ttcccccgca gcccacccgt tttcgggagc ggcggccagg
     1381 ttccagatca ggcaggtgag agttgccaga taaagcaccc acagactctt cacgggaccc
     1441 tcctcattgc gttggaaccc cccggggccg ggatcctcgg caccggcaac cgtgcgggaa
     1501 cggactccca ctatcattgc atgaggcacc cgcggcgagt caagggaaaa cacgggggtg
     1561 cgatcacgtc ttttccccgg acatcccccg ggaatcctcg gccttcacga ggctccccgc
     1621 aacccctcca cccgggggag aagcgggaag gatcgcagcg gggacggaat cggagcgggt
     1681 ttttcggtct tgactccccc cgataccgcg tgctagaagg ctaaggatta tttggtggcc
     1741 ggatgggccg ttaactcagg aggtagagta tctgcctttt aagcagagag tcgccggttc
     1801 gaatccagca cggcccacca ccaaaccatc ctgccgggat ttccggcacc cttccgaccc
     1861 ggcaaccggc ccccggtttt cagaaacacc ttgacaacgg aaggggcatt gagtagaaaa
     1921 cacgggcttt tggtccccat cgtctagcct ggtccaggac accggccttt cacgccggca
     1981 acaccggttc aaatccggtt ggggacgcca aacgattcca acgaggttga gttctgaaat
     2041 accttcaaga ccgtccctgg atagaggggt ggatagagcc aggccttccg agcgtcctct
     2101 cggaaggtct tttctttttc ccgcatggtg tttcttcgaa gagggatggt caggaaccgg
     2161 gtgcgggcac ttccatggaa gatttcggta ttgaaccggt tgcccatggg gggtagattg
     2221 tcccctgtag gcttcaagtg aattcaaggg tctcccccga aggaaatcaa gaattcaatg
     2281 ctctcctgtc ccgcggctgc tgtggctgca agggaggtcc cttgcccgga gggtgaccgg
     2341 aaagtggatg agtacggttc atcgaagcga cgcattcctt tgggcgtggc gtcgttctcc
     2401 acgggcttcg gggagacgtg ctgagaacgg aaggtttgag ggcatgggac cccaaactct
     2461 ggttcatccg gtatgtaagt tgtgggcggc aatcacaaga gacaggggcc actgctatga
     2521 ttttcctgag cggatcgcct cttccggaca aggataaggg tcgcaaatcc caccatacct
     2581 tgccaccgag aatctcaccc ctcttctttg ggagtgctcc tgctgtatgg aacggcaacc
     2641 acccatagca aggtccggca accggttgat gagccatggc ctggacctgg aaagaggtta
     2701 cggaccgtcc aaacaggagc tgatctcttt tctcgagaaa agggcctgac actggattgc
     2761 gttctcaggc aggaatcggg acggtcccgg acactcgggg ccgcgacgca aggctgcgaa
     2821 caagcatgac tcagcagacg ccggccagtg tttctttgag ccgggtctct gaaaaattcc
     2881 tgagccgccg gcctcctgat cgagtacgga gaacatccat ggcgaggatc cgaatgaaga
     2941 tcccgacctg gcaatttgac tgcaatgcga cgcggcaact gccggaccga ccgtcgagac
     3001 gcgacgacgg gatcttgcgg aaaagcaaac tcgaaccggg aagacggatg gagatatcct
     3061 cgccccttta tcccgatgta ttttggcgta ttcccatagg cgttcacaag ggagtaccga
     3121 gagaatcacc ccggcgggac ctggaggatg attgggtggg gtggtacctt ctctcgcgcc
     3181 ccgaacccga ccggatatcg cggatcgcag gcatccccgg ggggcgcccg caagggcgat
     3241 taccccgtcg gcaaaccgag ggcgccttca gccctccctc agcccgtgcg ttcattggac
     3301 ctcttgcccg cctttcccag agggaccagg atgccgtcct tggcaccgaa tccccggcat
     3361 tttcgaagat tgggaagaag caggtcgatt cggttggcgt gcctggcggc catcaggtcc
     3421 tggtactcga ccacctggga ttttccgtcg atccagagct gaaatttgtc gccgaaacgg
     3481 taatgctggg caaggtcccg gctgagagcg atgatttttc cgtaatgccg ggagcgaatc
     3541 tgaagaagag aggcggtttc attgggggta ctgtccgtgc actccggaac attcgtgtag
     3601 gcggtgatgg tgaccttgtg ggccggagga acctgcttgc gaagctcctc ctcggaattc
     3661 acccctgcaa aggacggaag gaccagtccc agaaacagga cgaggggaaa caggggaagc
     3721 tttctcgggg gctttctctt ccgcaaacga taccggaaga aagatacggc gaccgagtgg
     3781 tcgtccatgg tgaggccaaa agggacacag ttggctgaag aacctgtttt cagggacatg
     3841 ccgggaaatg cctccctacc ttaggtcgga atcgagcccg ggtggcggcg gtttggaccg
     3901 caggatgcaa gagcgcacgg catgcccacg gactcggccc tcccggatgg gtttcgcatc
     3961 ttcctgcaac cgaaacatcc attttgacgt tcccggagag gcgaaagtct accgatcttt
     4021 caaaaacccg gatcatcgac tcgcccagag gtttctccgg ttgatttcat ccggaaccct
     4081 actattgact gaagtgagga ttcggacctg ccgggaacgg gagccatcga tgcggggggg
     4141 cctggcagca ggctctccat acgacaagga gacggccgtg accgtttacg actacgatat
     4201 cggggtcatc ggcggaggcg ccgccgggct gaccgttgcc gcagggaccg cccagctggg
     4261 agccggaacg cttcttatcg accgggaacc ccggctgggg ggagactgtc tccattacgg
     4321 gtgcgttccc agcaagaccc tcatccgcac agcccacgtg taccacctca tgagaagggc
     4381 cgctgaattt gggcttccgt ccgtggacct gccgcctccc gactttctcg agatcgcccg
     4441 acgaattcgg tcggtcatcg gggagatcca acggcacgat tccgtggaac gattctgcaa
     4501 actgggcgca aaggtcgaat tcggagaagc cacgtttgcg gacgagcatt ccattcgtct
     4561 caacggctcc cttccctcgg caagacactg ggtcgtcgcc acgggttcct cccctgccat
     4621 ccccgccatc aaaggcctcg atgaaacccc ctacctgacc aaccgggaga tcttctccct
     4681 cgatcgcctg ccccggtcct tgatcattct gggaggagga cccatcggaa tcgagatggc
     4741 ccaggccttc gcgcgcctgg gcaccgacgt ccgggtggtg gaatccggcc cccggatcct
     4801 gcccagggac gaccgggacc tgacggaccg tgtcatggag gttctcgcct ccgaaggagt
     4861 ccgattttgc ctgtcctcga gagccgtgga ggtacgcaac ccgggcgggg aacgccaagt
     4921 ggtcctcgag gacccaaacg gccagaggac ggtggtccgg gcggaggagt tgttggttgc
     4981 cgcgggacgg gttgccaata tccgggggct gggcctcgaa tcactgggaa tcaagttcga
     5041 tgtcaaaggc atttccgtgg attcgagact gcgcaccgcc cggaaacaca tctacgccgc
     5101 cggggacgtc acgggcctgt atcaattcac ccatgccgcc gggtacgaag ggggcatcgt
     5161 ggttgccaac accgttttcc gccttccccg gaaaacggat taccgccggc ttccatggtg
     5221 cacgtacacc gaccccgagc ttgcgagtat cgggatgaac gaaggcatgg cctcgagggc
     5281 cgggatcgag tattccgtat ggacggaaga atttgaggca aacgaccgaa gccttgccga
     5341 gggagcccgg gacgggaaga tcaagttgat ccttgatgcc cgggaaaagg ccctggggat
     5401 tcagatcctg ggaccccggg ccggggaact cctcggcgaa tgggtggcgg ctctgaacgg
     5461 tcaggtgaag ctgaccaccc tggcatcgtc cattcatccc tatcccaccc tcggggagat
     5521 caacaagaag gtcgtgagct cctacctttc ccccaagatc ttttcggaaa ggatcaggaa
     5581 aggacttcga ctgttcttca acctcaaagg cagggcttgc ggaacggaga cgacctcgag
     5641 ctgagagcgg acccttgcct tcccgccgcg gcacggtgca taatgccgcc gggtcttctt
     5701 ccactcttgc gatcgaggca cggatttgga cattgcgcag ctcacgggat tgattcagct
     5761 cctcatggac ctcgcccaaa aggtggctcg gggagagtac gaccgcgccg acgaactgct
     5821 cgaactgacc cgtcaggacc gctatccggc catgattcgg gacctggccg aatccttcgg
     5881 gatgatgctg gtccaggtcg aggccaggga atttcacctc gagcagatga tcgacgagct
     5941 ggcggccaag aaccggcaaa tcgaggcaac cctcaagaag gtgcgccttc tcgagaatat
     6001 ccgggatcag ctctccaagt tcgtgccctc ttcggttcgg aacctcatcg agcaaaatcc
     6061 ggaaaacccg gacctggaaa aacgagacga ggacgtcacc gtgctcttcc tggatatcgc
     6121 cgggtacacc cggctcagcg aacaggtgga accggaaaag gtcaactacc tcatccagac
     6181 ctatttctcg agctttctcg acgtcatctt cgagaacgag ggagacatca acgaaacggc
     6241 gggagacggc ctgatgatcc tctttcgcga tcccgacggg cgtgcccatg ccgccaacgc
     6301 cgttcgagcc gccgtgggaa tccgggaccg ggccggggcc atcaacgagg ctcatcgagg
     6361 cttgtacgcc ccggtcatcg tcaacatggg catcaactcg ggaccggcct ccgtgggctc
     6421 gacccggttc gaaggagttt ccggaactcg atggaccttc accgcatcgg gtcccatgac
     6481 taataaggcc gcccggatca gttccctggc cgccgacggg gccatcctta cggggcatga
     6541 aacctaccgc cgggtcgcca aaacctttcc cctggaaccc ttggggcggc atcgactcaa
     6601 ggggattcag aaaccgatcc tcgtctaccg ggttctctag gcggagcctc cttctgcctg
     6661 cttttcggga ctcgaatcgg gaaggttttc cgactctccc gctgtttgca ggcggaaggt
     6721 catcgtcccc acgtcctttc caacaggttc cccggcgcct tcgctcctcg atccaaccct
     6781 agcctcggac gattctcact tccacccccg tctgactttc gagttcctgc acgtgatctt
     6841 ccacacttcc cttgaaaagg atcttttcga aaaaggtgcg gtcttcgtgg ccgagaacaa
     6901 gcagatccgc ctcttcctcg agaacgacct gcttgaggac gtccaacacg gggccctttc
     6961 gcacgacctt tttgggagtc accccttgag acggggcgat ttcgtcggca aaatccagca
     7021 tatggccgcc caggtgcgcc agggactctt ccgcaaattc cgaacccagc tgcaccgcct
     7081 ttccagtctt gagaaattcg acatccaccg cgtatacata cacaagatcc gcttgcgctt
     7141 ccccggcaag gacagctgct tccagcgccg ccctccggga aggaatggat ccagtcactc
     7201 cgcagacgat cgtcttgtaa gccatgattc gttccctcct gttggaatgg tctccgagcg
     7261 aatcgtgcat caccgctctt tgagggtcgc ggggacaacg ctgggtcaag gcggagacgg
     7321 gtcatcgtct ttcccggccg atgccaccgc gaggagccag gcaacgccga tggtgccggc
     7381 caggagtgaa gccagcagga ttcccacctt ggcctggatc gccatgccgg gatcgtcaaa
     7441 agccagttca ttgatgaaaa gagacatggt gaacccgatc cccgcaagcc atgcaacccc
     7501 cacgagatga tgccagcaga cgccgctggg gagccgggcg aacccgcatt tcaccgcaaa
     7561 ccagctgaaa ccggaaactc ccgcaaattt gcccaggacc aggcccagga cgatccccca
     7621 ggtgaccggc tgtccaagga cgcggctaac ctccatgttg ctgaaattca ccccggcatt
     7681 gctcagggcg aaaaggggaa tcaccacgaa ggtcacccag gggctcaggt gatgctccat
     7741 tcgctgcaac ggcgattgaa ccgccttggc cgacttctca acgttttgag ccaccgtagc
     7801 catcacgggg catttcaggg gttgaagaca ggcttcgggg tcttccacct cgtaacggag
     7861 cgtctcctgc aggtgatcca gccgctgttc gaattccacc ggggtgaaca cgggacgggc
     7921 ggggatgaaa aacgccagca gcaccccggc caccgtggcg tgaatccccg agtgcagcaa
     7981 tacaagccaa agggccatgc ccatcacccc ataaggaagg ggatggcgga ttccccctcg
     8041 attgagcaac gccaggactg ccaatatggc caatgctccc ttgagggcac cgatatccag
     8101 ggaggtcgag tagtagaggg caatgagaag gaccgctccc aagtcgtcgg caatggccag
     8161 cgcagccaag aagacgatga gactcttggg gattcgccag gaaagcagga ccaggatgcc
     8221 cagggaaaag gcgatatccg tcgccatggg gattccccag cccttggctt cgaccccatc
     8281 gcggtttacc aagatataca ggatggccgg gacgaccatt cctcccacgg cggcaaccac
     8341 cgggaggaca gcctcccgca ggctggacag ttcccccacg aggacctctc gtttcaactc
     8401 cagccctacg aggagaaaga aaagggccat gagcccttcg ttcacccagt gatgcagggt
     8461 catctcaact cgaaaaccac cgatcccgat cccggcacgc tgttcccaga agtcgtgcag
     8521 gaactgtccc caaggggaat tggccagcaa cagagtcgcc acggtgacgc tcatgaggat
     8581 gattcctccc gccgtggcgc gcctgagaaa ctgctcgaag ggactgagca tcctgccgaa
     8641 tagaggttcc aggatgtaaa cgttctcttt catcgcgtcg tgcgcttttt cttgggttcc
     8701 tcaaccgatt cggcacgccg ccgcaccagg cgagcgcttc ccataggtcc gaaggatcct
     8761 ctccttgggg cgaaccccgc atcggtaccg gggccgaaat ccaccccaac cgccccggct
     8821 gacggtcttt gcttctttag gttgatcaag gacttccaat ctgcgggacg aaacccgggt
     8881 ccggtcccct gatgctcggg gcccatcggg cggtcgtcgg ttcctgcttc gattcgtacc
     8941 ccacaccggg gacggccttg ccgggcgccg cccccgcatt ccaataaact gatacgcccc
     9001 ggctgggagg tcaagggtta tcggcctttg ggcgcctgtt ttcgaggcgg caatcaccgg
     9061 gttgcgttga ccgtcaggga aagcactctt gcagaaagga ggatttccgc ccggcggacg
     9121 gcgggttggt ggttgggagg aacgaggaga attgcgccaa tccgatggta ccgctcggga
     9181 caatcgaacc gccatttaac cggattctgc gtgagctggg agcaggagct ttccgggaag
     9241 cggagtaact tcctttgggg ggaggatggt tcccgcaact cccgtttcgc tcccggagca
     9301 aatcgatgga gatcgaagat caacaagggg ttcgattccc tgcttccgcc tctgaatagt
     9361 ccgcttgcgc tcttctcctc gtcggcttcc cttagagcag aaagcatgcc ggcattcttc
     9421 ccgaaatccg ccgcatcttg aagagcggga ccgcatcccc ctttgtattc gggaggctcc
     9481 ccgggatagg gggttcggga ggaatcggcg cgggaggatt tgtccgattc cggggactct
     9541 ctccgaaaaa ccggaccttt cttcggcagc atgaagggga tcgtccttcc ggcggacccc
     9601 gggtttagtc cggggcagcc gccggaattc tcgagagagg caaggacggc gtccttggcc
     9661 gaaaggacgg gtattgtgga cccccggttg gaagagaccg ggctaagggc aattgatcct
     9721 cttcaaagtc cccctcgaaa aatgattgct ataggtcttg gaattgaggt cgtagtccgt
     9781 tccgacttcg tagaaggtac ctttcatggg cgaggtggag gtattcaact ggatctgcat
     9841 gattcccgtg tcccgccacc cgtccttgtg caactgggtg ccggtgaggt tcaaaatcac
     9901 gtacttgccc gcc
//
LOCUS       DAOOVC010000144         9880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4350_length_9880_cov_6.367532, whole genome shotgun sequence.
ACCESSION   DAOOVC010000144 DAOOVC010000000
VERSION     DAOOVC010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9880
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4350_length_9880_cov_6.367532"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(121..1080)
                     /locus_tag="PLM79_14690"
     CDS             complement(121..1080)
                     /locus_tag="PLM79_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOV87605.1"
                     /translation="MATCRSVALKVDVDTHDGMRRGVPVLLEVFRRFSVRATFCLAFG
                     PDNAGKALYRVLKDPVFFRKMLRTGAPRLYGWRTILSGTLLPARPTASGFPDLVRQIR
                     AEKHEVIVHGWDHRLWQDHVAAMDESSIRSQFQQAFCAFEQILGVRPRAVAAPGWQAT
                     PRSLRVQDKLNLLYASDLRNAEPCFLEAGGHGFRTLQIPTTGPCVEELLAGGIREEKA
                     ILDALTAPLEAYSFPVLALHAEVEGGPFRGLLERLIPLLRNRYERFSTLEEEARKRLS
                     DGEPIPRRELVFIRLPGRAGRIASASGPPSLVGSGRAGGGRPV"
     gene            complement(1080..2135)
                     /locus_tag="PLM79_14695"
     CDS             complement(1080..2135)
                     /locus_tag="PLM79_14695"
                     /inference="COORDINATES: protein motif:HMM:NF008872.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HOV87606.1"
                     /translation="MKILVLGVNGFIGSHLVRRAMAETDWEVYGMDLADDRLGPLVNN
                     PRFHFIEGDVSINKEWIEYHVKKCDVCLPLVAIATPATYIRDPLTVFELDFEQNLRVI
                     RQCARYGTRVVFPSTSEVYGMCPDSVFNESTSNLVYGPIHKQRWIYACAKQLLDRVIW
                     AMGLHSDLRFTLIRPFNWIGPGLDSLATPKEGSSRVVTQFLGHLLRKEPITLVDSGRQ
                     RRCFTYVDDGLDCLLAILRNENRCCDGKIFNIGNPDNDYSIRDLAELMVEVVREFPNL
                     AHLADEARFQELPADHYYGAAYQDVAYRVPDISTAREILGWEPRTDLREALRLTISAY
                     VQDQEDSVRKLGSPDEL"
     gene            complement(2207..3124)
                     /locus_tag="PLM79_14700"
     CDS             complement(2207..3124)
                     /locus_tag="PLM79_14700"
                     /inference="COORDINATES: protein motif:HMM:NF005414.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HOV87607.1"
                     /translation="MSVVLFGYHNMGCIALKVLTELGIPVNAVVTHRDDPNENRWFES
                     LAEKAGEKKIPVHYSEDLDSPGLLALVRTLAPEVLLSCYYRHMISEKVLAVPRRAALN
                     LHGSLLPRYRGRCPVNWQILHGETVGGVTLHHMVRQADAGDIVGQRAVAIAPDDTAYA
                     LFRKLEPAAEELLREMMPRVMEGTAPRIPQDHSLADYFGGRRPEDGRIDWEWPAGRIY
                     NLIRAVAWPYPGAFSILDGKKILVWWGEVREGGETHGPPGTVTAVGEERWVQTGEGRL
                     KLVTLSWEGGSPDRGFPPHEVLREGDRLL"
     gene            complement(3127..4833)
                     /locus_tag="PLM79_14705"
     CDS             complement(3127..4833)
                     /locus_tag="PLM79_14705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV87608.1"
                     /translation="MTGAEGAQQRRDPAWDCLLYVVAAFLLFWDLGNRGLWVAEGRWA
                     VIAREMLATGDFFHPTINGEPYFDKPLLTYWWIVASSFLTGGVNEWAVRLPGALSGFA
                     AIFCTVRLGSRLWSPEVGRTAGWLLLSSYGFLFWARTGTAEMENLAAVVLAVTWYWVR
                     KDRTDFFTYLVFYLVLFLGAHNKGLGAVVVPVLVLFPDLLMEGRGRILFRPAHLAALF
                     IGVAVYLVPFLVASATRPDYGAAGLDLVFRENIRRYYDPFDHTAPFFMYLVWLPSLLL
                     PWTPLFLASLGSLLPSWKSLDRASRWPARAMMLTFVFYTLSGSRRSYYILPILPFCAL
                     WISAFLCRQDEPGSRIPWKRWGLATTEAAFLVLAVGAMMSPLVRPWIESRTGFIPPAG
                     LQAGLILCGAFCFGVWALSRRKKDFWSRLTGLNQGLAPLGVMAVAVLGAYFCWQQGVL
                     EVYRTEKNFSLALRQVVKDIAPGDIGFYRKYATNVLFYLGSPRPVKVLEDADSVAEFL
                     RSSPPVKVLVSRRGDLPTLLPLLPPPAREGFVLEERSEPWEDKGAWKNKLVAVHLRPT
                     QE"
     gene            complement(5048..6085)
                     /locus_tag="PLM79_14710"
     CDS             complement(5048..6085)
                     /locus_tag="PLM79_14710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87609.1"
                     /translation="MKGIIGIMEQQDAAATSTRPLGTVECGPPAPGPQVSIVTPMFNE
                     EANVVRFCERLFAALGTISESYEVICVDDGSRDRTLELLKREQIVRKNMTVVVLARNF
                     GQHAAVMAGLAASRGDWVITLDADLQNPPEEIPRIVEAFRQGHDLVGTIRTGRQDPLF
                     RKWASRFTNRLVTRISGISLQDFGSMLRGYSREVVQGILANPEFRTFIPALSTFFARK
                     SVEIPVRHEERAGGTSKYSLLKLFSLQLDLVTGFSLWPLRALFFVGTGMAGLGIGLAL
                     LILSFRLYYGSGWAAEGVFTLFAILFCFVGAQFLALGLVGEYVGRIFQAVRRRPPYIL
                     SEIRRNGPFMP"
     gene            complement(6082..7266)
                     /locus_tag="PLM79_14715"
     CDS             complement(6082..7266)
                     /locus_tag="PLM79_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV87610.1"
                     /translation="MTDHLPVRSSFLAFSRPTIGQEEIDEVVDSMRSGWLTTGPKVAR
                     LEGLFAEWSGGREAVAVNSATAGLHVAVAALDLRPEDEVITTPITWPATVNNIELCGA
                     KPVFADVDRETLQIDPREVERRIGPRTRAIIPVHFAGAPCDMDPLREMSRKHGIRLIE
                     DAAHAVGTAYKGRPVGSDADVAVFSFHPIKNMTTGEGGMILCADAAMAARMRRLRFHG
                     ISRDVWKRYSKGGTPQFEVDEPGFKYNMLDLQAAIGLHQFAKLEGFNERRGELAARYD
                     ELFSRVPEIRPLGRAAYPHRHAWHLYVVRLELEAVNLTRDEFLAKLQEEQIGTGLHFP
                     AVHLQRFYREKYGYAPGDYPNAEWNSERLFSIPLYPLLEEQDQLDVVEAMRRIIRRHR
                     KA"
     gene            complement(7263..7637)
                     /locus_tag="PLM79_14720"
     CDS             complement(7263..7637)
                     /locus_tag="PLM79_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HOV87611.1"
                     /translation="MNRFLPLILLGVLLNAVAQLALKQGMRGIGPFAFAWENVLPIGL
                     RVSLNPFVLLGLFCYIVSVVVWLMVLSRVDVSFAYPFLSVGYIVTALAGQLFLGEHVN
                     VTRWAGIFVICFGVYLITRSGS"
     gene            7944..9338
                     /locus_tag="PLM79_14725"
     CDS             7944..9338
                     /locus_tag="PLM79_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOV87612.1"
                     /translation="MIRYAIARPLAGLVALSLCLFLAGGLDAREDLKPQIDAVAEPLL
                     EHRMTVGVAVGLLEGDSVREFGYGRISRQTDRKPDSRTVFEIGSVTKVFVTLALSDMV
                     EEGRVKLSDPVAVYLPDSVRAPQFEGRQITLGHLAEHTSGLPRIPNNFTPSNPLNPYA
                     DYTAEKMYDFLSAFTLPRAPGSCYEYSNLGAGLLGHVLARHTGLDLGALLQERICRPL
                     NLVDTSIRVSPDGQLRLTPGHDLDANPVPYWDFDVLAGAGALHSTVADLVLFVKANMG
                     LLPTPLFAAMEKCRAPRMVTTLGDWHIGLGWHVSADGNTAWHNGGTYGFYSFIGFNRE
                     RKVGLVWLSNSSLWQIPDVNARLLDILAGRSAEPMKFKTPVPLPAETLDRYVGEYRPA
                     PDATLRVRRDGTRLILQNPEGAETALFPESQTRFFLRETSETTVEFVLDEGGNTVRMD
                     LHGKEGDKSAARVR"
     gene            complement(9454..9798)
                     /locus_tag="PLM79_14730"
     CDS             complement(9454..9798)
                     /locus_tag="PLM79_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HOV87613.1"
                     /translation="MSVVEKLYEALKSIQEPKGYYFNKDREKVFQLLEGLIQNRERYG
                     YMCCPCRLASEDREWDRDITCPCEYRAADVAEYGSCYCNLYVSKEWNQGTVPHVFVPE
                     RRPVEKTLGKLL"
BASE COUNT         1958 a         3140 c         3046 g         1736 t
ORIGIN      
        1 cttccggcac cggaccggct gcggacccgt cgtccggacg ccgacgggcg accttccctt
       61 cctcccggca ggactggggc gaatgggcgc actggagaga aatcccgtct tcgatccccc
      121 tcacaccggc cgacctcccc ctgcccgccc tgaacccaca agggatggag gtcccgaagc
      181 cgaggcgatt cgtcccgccc tcccgggcag ccggatgaaa accagctccc gccgggggat
      241 gggttcgccg tcgctcaaac gtttccgggc ctcttcctcg agggtcgaga accgttcgta
      301 tcgattcctg agcagcggaa tgagccgctc gaggaggcct cgaaagggtc ccccctcgac
      361 ttcggcatgg agagcgagga ccggaaacga gtacgcctcg aggggagcgg tcagggcgtc
      421 gaggatcgcc ttctcctccc ggattccccc tgcaaggagc tcttccacgc acggccccgt
      481 ggtggggatc tgtagggttc gaaacccatg ccccccagcc tcgagaaaac acggttccgc
      541 gttcctcagg tcactggcgt aaaggaggtt cagcttgtcc tgcacccgga ggcttcgggg
      601 ggtcgcctgc caaccgggag ccgcaacggc tcggggtcgc acgcccagga tctgttcgaa
      661 ggcgcaaaac gcctgctgga attgggatcg aatggaactc tcgtccatgg cggcaacgtg
      721 atcctgccag aggcggtggt cccagccgtg gacgatgacc tcgtgttttt cggcgcggat
      781 ttgtcggacc aggtccggaa atcccgatgc cgtgggccgg gccggaagca aggtccccga
      841 aaggatcgta cgccacccgt acagtcgggg ggcccccgtg cgaagcattt tcctgaaaaa
      901 gaccggatct ttcagcaccc ggtacagggc cttgccggca ttgtccgggc caaacgccag
      961 gcaaaaggtg gcccgaacgg aaaaccgccg gaagacctcc agcagtaccg gtaccccgcg
     1021 gcgcatgccg tcgtgggtgt ccacatccac tttgagggca acggagcggc aagttgccat
     1081 tagagctcat ccgggctgcc cagtttcctg accgaatctt cctggtcttg aacataggcg
     1141 gaaatggtga gtcgaagcgc ttccctgaga tcggtcctgg gctcccatcc caggatttcc
     1201 cgggccgtgg agatgtcggg gacccggtaa gcgacgtcct ggtaggcggc tccgtagtaa
     1261 tggtcggcgg gaagttcctg gaaccgggcc tcatcggcca ggtgcgccag gttggggaac
     1321 tcccggacca cctcgaccat gagctcggcc agatcccgaa tggagtagtc gttgtcggga
     1381 ttgccgatgt tgaagatctt gccgtcgcag caccgattct cgtttcgcag gatggccaga
     1441 aggcagtcca gtccgtcgtc cacgtaggtg aaacagcgac gctgtcgtcc gctgtccacg
     1501 agagtgatgg gctccttgcg aagcaggtgt cccaggaact gggtcaccac ccgggagctt
     1561 ccttccttgg gggtcgccag gctgtccaaa ccgggcccga tccagttgaa aggtcgaatg
     1621 agggtgaaac gcaggtcgct gtggagcccc atggcccaga tgacccgatc cagcaattgc
     1681 ttggcgcacg catagatcca ccgctgcttg tgaatggggc cgtacaccag gttgctggtg
     1741 gactcgttga agacggaatc cgggcacatt ccgtagactt cgctggtgga gggaaatacc
     1801 acccgggtcc cgtaccgggc gcactgacgg atcacccgga ggttctgttc gaagtccagc
     1861 tcgaacacgg tgagggggtc ccgaatgtag gtggccgggg tggcgatggc caccaggggc
     1921 aggcaaacgt cgcatttctt gacgtggtat tcgatccatt ccttattgat ggacacatcc
     1981 ccctcgatga agtgaaaccg gggattgttc accaacggcc ccagccgatc gtcggccagg
     2041 tccattccat agacttccca atcggtttcc gccatggccc gacgaaccag gtggctcccg
     2101 atgaaaccgt tgacgcccag aacaagaatc ttcacggttc cacgcactcc ttttctagat
     2161 tcaggtttcc cgggtcccgg ccgacctgca ccgggctaca gggttctcaa agcaggcgat
     2221 ctccctcgcg gaggacttcg tggggcggaa aaccccggtc cggagacccc ccttcccagc
     2281 tcagcgtgac cagtttgagt cgcccttccc ccgtttgcac ccaccgctcc tctcccacgg
     2341 ccgtcacggt gcccggaggc ccgtgggtct cccccccctc ccgcacctcg ccccaccaga
     2401 cgaggatctt tttcccgtcc aggatcgaaa aagccccggg gtagggccag gccacggctc
     2461 gaatgaggtt gtagatgcgc ccggcgggcc actcccagtc gatgcgcccg tcttcaggcc
     2521 gcctccctcc aaaatagtcg gcaaggctgt ggtcctgggg aattcgcggg gccgttccct
     2581 ccatcactcg aggcatcatc tctctcaaaa gctcctcagc ggcgggctcg agcttgcgga
     2641 acagcgcgta ggccgtatcg tccggggcaa tggccaccgc cctctgcccc acaatgtctc
     2701 ccgcatcggc ctggcgcacc atatgatgga gggtgactcc cccgacggtt tccccgtgaa
     2761 ggatctgcca gttgacgggg catcggcccc ggtatcgcgg caacagggac ccgtgcagat
     2821 tcagggccgc ccggcgcggg accgccagga ccttctcgga aatcatgtgc cggtagtaac
     2881 aggagagcag gacctccggg gcgagagtcc ggaccaatgc caggagtccc ggggaatcca
     2941 ggtcctcgga atagtggacg gggatctttt tctccccggc cttttccgcc agggattcaa
     3001 accatcggtt ctcgttggga tcgtcccgat gggtcaccac cgcgttaacg ggaatgccca
     3061 gctcggtgag aaccttgagg gcgatgcagc ccatgttgtg atagccgaaa agaacgacgg
     3121 acatggtcac tcctgcgttg gacgaagatg gacagccacc agcttattct tccatgcccc
     3181 tttgtcctcc cagggttcgg acctctcttc gaggacaaac ccttccctcg ccgggggcgg
     3241 caagaggggg agcagggtcg gcagatcccc ccggcgggaa accaggacct tgaccggcgg
     3301 agaagaacgg aggaattcgg ccaccgagtc ggcgtcctcc aagaccttca cgggccgggg
     3361 agatcccagg taaaagagca cattcgttgc gtattttcga tagaaaccga tgtccccggg
     3421 ggcaatatcc ttcacaacct gccgaagggc cagggagaaa ttcttttcgg tgcgatacac
     3481 ctcgaggacc ccctgctgcc agcagaaata ggctcccaat accgctaccg ccatgacccc
     3541 caggggcgcg agcccctggt tcaatcccgt gagtcgactc cagaaatcct tcttccgccg
     3601 ggaaagagcc cagaccccga agcagaaggc cccacagagg atcaagccgg cctgcaaccc
     3661 cgcaggggga atgaaaccgg tgcgactctc gatccagggc cgcaccaggg ggctcatcat
     3721 cgccccgacc gccagcacga ggaatgcggc ctccgtggtg gcgagccccc agcgcttcca
     3781 ggggatccgg gacccgggct catcctgccg gcataggaac gccgaaatcc acaaagcgca
     3841 aaagggcagg atgggcagga tgtagtagct tcttctcgac cccgagaggg tgtagaaaac
     3901 gaaagtcagc atcatggccc gggccggcca gcgggaagcc cggtccaggc ttttccagga
     3961 cggaaggagc gagcccaggg aggcgagaaa aaggggcgtc cacggaagga gtagggacgg
     4021 cagccacacc aggtacatga agaagggagc cgtgtgatcg aagggatcgt agtagcgccg
     4081 gatgttctcc cggaacacca ggtccagccc ggcggccccg tagtcgggtc gcgtggccga
     4141 ggccaccagg aagggaacca ggtagacggc aacgccgatg aaaagggccg caaggtgtgc
     4201 cgggcgaaaa agaatccgcc cgcgtccctc catgagaagg tccgggaaca ataccagtac
     4261 gggcaccacc acggcgccca gccccttatt gtgggccccg agaaaaagga ccaggtagaa
     4321 taccaggtaa gtgaaaaagt cggtccggtc ttttcggacc cagtaccacg tcacggccag
     4381 gaccaccgcc gccaggtttt ccatttccgc cgtgcccgtt ctcgcccaaa agagaaaccc
     4441 gtaggaggac agcaggagcc aacccgccgt gcggcccacc tccggggacc aaagtcggct
     4501 tcccagccgg acggtgcaga agatcgccgc aaagccggac aatgcgccgg gaagccggac
     4561 ggcccactcg ttcactcctc ctgtgaggaa agacgaggct acaatccacc agtaggtgag
     4621 cagaggcttg tcgaagtagg gctcaccgtt gatggtgggg tgaaaaaagt cgcccgttgc
     4681 gagcatctcc cgggcgatga ccgcccatcg accctcggcc acccacagac ctcggtttcc
     4741 caggtcccag aacaggagaa aagccgccac aacgtagagg agacaatccc atgccgggtc
     4801 gcggcgctgc tgtgcccctt cggcacccgt caatccgcac ccccttccgt tgcgggattc
     4861 ggagagggtc cgaccggtgg gccgccagcg tcctcccggc caagaccggg gtccgaaaac
     4921 cgacggaaaa cgtgaattcc ggcctccacc ggaagggcga gctgcctggg acccatcatg
     4981 gacacccgtt tctacgatgt gcggttatcc gcgcttggta cgacacaaca cgggaatggg
     5041 gcggggatca gggcatgaag ggcccgttcc gccggatttc cgagagaatg tagggaggac
     5101 gccgccgcac cgcctggaag atccttccga cgtattcccc caccagcccg agagccagga
     5161 actgcgcgcc gacaaaacag aacaggatgg cgaacaaggt gaagacccct tccgcggccc
     5221 agccggaccc gtagtagagg cggaaggaca ggatcagcaa cgcaaggccg atccccagac
     5281 ccgccatgcc ggtccccaca aagaacaatg cccgcagggg ccacagggag aagcccgtga
     5341 ccaaatccag ctgaagactg aaaagcttca ggagggaata cttggaggtg cctcccgccc
     5401 gttcctcgtg gcggacggga atttcaacgg attttcgggc aaaaaacgtg gagagggccg
     5461 ggatgaaggt gcgaaactcc gggttggcca gaatcccttg gaccacctcc cggctgtagc
     5521 cccgaagcat actgccgaag tcctggaggg agattccgga aatgcgggtt accaggcggt
     5581 tggtgaaacg cgaggcccac ttgcggaaca aggggtcctg cctgcccgtg cggatggttc
     5641 ccaccaggtc gtgtccctgg cgaaacgcct ccacaatgcg cggaatttcc tcgggaggat
     5701 tctgaaggtc cgcgtccagg gtgatgaccc aatcgccccg gctggcggcg agtccggcca
     5761 tcaccgccgc atgctgcccg aagttgcggg ccagaacgac aacggtcatg ttcttgcgga
     5821 cgatttgctc ccttttcaac agctccaggg tacggtcccg ggagccgtca tccacgcata
     5881 tgacttcata ggattccgaa atggtcccca gggcggcgaa cagccgttcg cagaacctga
     5941 cgacattggc ttcctcgttg aacatgggag tcacgatgga aacctggggg cccggggcag
     6001 gcgggccgca ttccacggta ccgaggggcc tggtactcgt tgctgctgcg tcttgttgtt
     6061 ccataattcc gatgatgcct ttcatgcctt tcgatggcgc cggatgatgc gcctcattgc
     6121 ttccaccacg tccaactggt cctgctcttc gagaagcggg tacaagggaa tggaaaagag
     6181 ccgttcactg ttccattcgg cgttgggata atctcccggc gcgtacccgt atttttctcg
     6241 gtagaaccgc tgcagatgga ccgccggaaa atgaaggccc gttccgatct gctcctcctg
     6301 gagtttcgcg aggaattcgt cccgggtcag gttgaccgct tcgagttcga ggcggaccac
     6361 gtagagatgc cacgcatgac gatggggata ggcggcgcgg cccaggggtc ggatttcggg
     6421 gacccgggaa aagagctcgt cgtaccgggc cgccagctcc ccccgtcgct cgttgaaacc
     6481 ttcgagcttt gcgaactggt gaagcccgat ggccgcctgg aggtccagca tgttgtactt
     6541 gaaccccggc tcgtccacct cgaactgggg agttcccccc ttggaatagc gcttccagac
     6601 atcccgggaa atcccgtgaa accgcagtcg cctcatgcgc gccgccatgg ctgcgtcggc
     6661 gcaaaggatc attccccctt ccccggtagt catgttcttg atgggatgga agctgaacac
     6721 ggccacgtcc gcgtccgatc ccacgggacg ccccttgtag gccgttccca ccgcgtgggc
     6781 ggcgtcctcg atgagccgaa tgccgtgctt gcggctcatc tcccggaggg gatccatgtc
     6841 gcagggagcc ccggcgaaat gaacggggat gatggctcgg gtacggggtc cgatgcgtcg
     6901 ctccacctct cgagggtcga tctggagggt ttcccgatcc acgtccgcaa atacgggctt
     6961 cgccccgcac agctcgatgt tgttcaccgt ggcgggccag gtgatggggg tggtgatgac
     7021 ctcgtcctcc ggtctcaggt ccagggcggc cacggccacg tgcaggcccg ccgtggccga
     7081 gttgacggcg acggcctccc ggcctccgct ccattcggca aagagcccct ccaatcgagc
     7141 cactttgggt ccggtggtca accaccccga acgcatgctg tccaccactt cgtcgatctc
     7201 ttcttggccg atggtgggtc gcgaaaaggc cagaaagctc gaacgaacgg gcaaatgatc
     7261 ggtcatgatc cgctccgtgt gatgaggtag acgccgaaac aaatgacaaa aatcccggcc
     7321 cagcgcgtga cgttgacgtg ttcccccagg aagagctggc ctgccagggc cgtcacgatg
     7381 tagccgacgc tgagaaaggg atacgcaaag ctgacatcca cccgggagag caccatgagc
     7441 cagacgacga cgctcacgat atagcaaaaa aggcccaaca agacaaaggg gttcagggaa
     7501 accctcaggc cgatgggaag cacgttttcc caggcgaatg caaagggacc gatccctctc
     7561 attccttgtt tcagggccag ctgggccacg gcgttcaaca gcactcccaa aagaatcaac
     7621 ggaagaaatc ggttcataca aggctctccc cgcgcaaggg tgaaatgggt gaaggagggg
     7681 tcccggcaac ccgctccctt ttagcgcttt tgtccgagga tgtcattacc gggatgccgc
     7741 cccgggagat tcgtccgggg cctatccgca aatggaactc atctccgacc gtctcccgcc
     7801 gggaaaagag ggagcttctc ccctcccggt tgcccggggt tgggacgggc gtggtttcat
     7861 ggatcaagaa ttccactcgc ggcagcccgt ttctgatatg atcgagggga cgattccatc
     7921 cacaaccggg gaggagaaaa gacatgatcc gttatgcaat cgcccggccc ctggccggtc
     7981 ttgtggccct ttctctgtgc cttttccttg cggggggcct cgatgcccgg gaggacttga
     8041 agccgcaaat cgatgcggtg gcggagcctc tcctcgaaca ccggatgacc gtgggcgtcg
     8101 ccgtcggcct cctcgagggc gactccgtcc gggaattcgg ctatggacga atttcccggc
     8161 aaacggaccg caaaccggac agtcgcaccg tgttcgagat cggttcggtc accaaggtgt
     8221 tcgtcaccct ggctctctcc gacatggtcg aggagggccg ggtgaagctg tccgaccccg
     8281 tggccgtgta tttgcccgac tcggtgcggg cacctcaatt cgagggccgg cagatcaccc
     8341 tcgggcacct ggcggaacat acctccgggc ttccccgaat ccccaacaac ttcacgcctt
     8401 cgaaccctct gaacccctat gccgactaca ccgccgagaa gatgtacgat ttcctgtccg
     8461 ctttcaccct gcctcgagcc ccgggcagct gctacgagta cagcaacctg ggggcgggac
     8521 tcctgggcca tgtcctggca cgccataccg ggctcgatct gggggccctt ctgcaagaga
     8581 ggatttgcag gcccctcaac ctggtcgaca cctccatccg ggtgagcccg gacgggcagc
     8641 tccgtctcac cccgggacac gacctggacg caaacccggt cccctactgg gatttcgacg
     8701 tgctggcagg ggcgggggcc ctccattcca ccgtggcgga ccttgtcttg ttcgtcaaag
     8761 ccaacatggg tctccttcca acgccgctct ttgcggccat ggaaaaatgc cgggctcccc
     8821 ggatggtcac caccctggga gactggcaca tcggcctggg ctggcacgtg agcgccgacg
     8881 ggaatacggc ctggcacaac gggggcacct acggtttcta cagcttcatc gggttcaacc
     8941 gggaacggaa ggtaggcctg gtctggctga gcaactcgtc cctctggcag atccccgatg
     9001 tgaatgcccg cctcctggac atcctggccg gcagatccgc cgaacccatg aagttcaaga
     9061 ccccggtgcc gcttcccgct gaaaccctcg accggtacgt gggggagtat cgccccgctc
     9121 cggacgcgac cctgcgggtg cggcgggacg gcacccggct gatcctgcag aatcccgagg
     9181 gagcggagac cgctcttttc cctgaatccc aaacacggtt tttccttcgg gagacttcgg
     9241 agacgaccgt cgagttcgtt ctggacgagg gcgggaacac ggttcgcatg gatctgcacg
     9301 gcaaggaagg agacaaatcg gcggcaagag tgcggtaggt ccgagctttc cccgcacacc
     9361 ggcgcctccg ccgacggacc ccggcgagca ccgcccggga acagggaatg tcgtcccatc
     9421 ccgggtcggg aaggtccgct cttgagggga ctcctagaga agctttccca gggtcttttc
     9481 caccggcctc cgttccggca cgaagacgtg gggaacggtc ccttggttcc attccttgga
     9541 aacgtacagg ttgcaatagc agctgccgta ttcggccaca tccgcggccc ggtattcgca
     9601 cgggcaggtg atgtcccgat cccattcccg gtcttcggaa gcaagcctgc agggacagca
     9661 catgtagccg tagcgctcgc ggttctggat gaggccctcc aggagctgaa acaccttttc
     9721 ccggtccttg ttgaagtagt atcccttggg ttcctggatg cttttcaagg cttcgtagag
     9781 tttttcgacc acgctcatag gtccagcgcc tccttgattt cctcttttcg aaaaccgacg
     9841 atgaccttgt ccccgatgat gagggtgggg aaagagcagt
//
LOCUS       DAOOVC010000145         9721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4433_length_9721_cov_7.055969, whole genome shotgun sequence.
ACCESSION   DAOOVC010000145 DAOOVC010000000
VERSION     DAOOVC010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4433_length_9721_cov_7.055969"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1452
                     /locus_tag="PLM79_14735"
     CDS             199..1452
                     /locus_tag="PLM79_14735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV87614.1"
                     /translation="MKKENTEPASPGNLRESPGLKWIVFSLVCAAFTTVYITQPVLPV
                     IRAEFHVSETRASWTIAAVVLGMALSNLPFGMLSDRYPLRPIILTGGATVASAGFLCA
                     ATSSLAVLVGARFVQGLFLPSLTTCLAAYLARSLPPASLNVVMGTYVSATVAGGLGGR
                     LLGGWIHPPLHWRFAFVSASLMLLGATLAAVKRLPGNGPVRPADTPAAGFLRLLSRKE
                     FLRIYLVAFGGFFAFSSVFNYLPFYLSGSPFGASTELITLLYLSYTVGMVVGPLAGKV
                     SNSIGNGNTMILGAGILGLSIVATLIPSLLLIIVSLVGICIGFFAVHASAAGSLNRKL
                     TGNQGRANSLYVLFYYLGGAVGIYLSGHAYALWGWAGVVGVTVSMLVIPLATGAAERR
                     EVGRALRKAGPLWMRSVQGSGLRKR"
     gene            complement(1551..1955)
                     /locus_tag="PLM79_14740"
     CDS             complement(1551..1955)
                     /locus_tag="PLM79_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87615.1"
                     /translation="MLGILSTLIRKTLSVRESTPWRNLGSGKLQGCRNSRVHETHEEK
                     TSVRQRLSALLRRLGLGLAVLALSLALGTPFPGTTSARGTLENPALFLAAEETEQIDT
                     NPFRPKIPEEYRFRYYRDNFAFRVPVLNLTDA"
     gene            2540..3736
                     /locus_tag="PLM79_14745"
     CDS             2540..3736
                     /locus_tag="PLM79_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV87616.1"
                     /translation="MQVFTAVCLFAFTVFAPAVSWATSPRSPDLGSAVSGTIKIGGAF
                     SLTGGGAALDLEAADGARLALTEINRAGKTLGKTMELVIRDSQSRPDLAEQIARQLVE
                     KDRVVAILGYSDTDSALAVGPVIRKHRIPFITVGATSPKLPEQVGEMMFLACFGDNVQ
                     AAAGAEFAFKHLGGGACLLWDKGMDYTRLLAGYFKRRFIELGGSVVIEEPFDDETPDL
                     SVQIQQLKSLPRSPDFFYIAAMPYNAGMVIRQLREAGFSAPVLGGDGFDTPQIVEIAG
                     PAAERVFFTTHALVDPTRGKEEMKRFITAFMAEYGREPRNSFAALGYDAMRLVVDAVR
                     RAGSTEPSSLKHALEETREFSGLTGTITLSTKSHIPQKSVTILEIKDGKFRWVEDLVP
                     QSVPSP"
     gene            complement(3895..4287)
                     /locus_tag="PLM79_14750"
     CDS             complement(3895..4287)
                     /locus_tag="PLM79_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87617.1"
                     /translation="MAYKSIFLLLWVILLFSQGAGFAKNVDPEKPFRDSWCAEHGGRT
                     DVLLPGGGRAHCVTETHAVEFSFAAHWDRALGRALVHGMRAGKTPGVVLILREEKEFK
                     NWIELVSTISHFNLPVTPWLTGKGAREY"
     gene            4489..5154
                     /locus_tag="PLM79_14755"
     CDS             4489..5154
                     /locus_tag="PLM79_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87618.1"
                     /translation="MRMTATSPNWKVLGLLVVVFAVGACAANGPYYTVSETPSAPPPP
                     PATTVAPPPVSTGLAVGRNEFNYSFDLIDRTPFNIVDLPRSLDILYRKGYDEVRQRGQ
                     ADLVVTVTLSAGAAENPEKRVGHAVGGALGGAAIGAIIGGALGDPGTGAALGAASGGA
                     AGALAPAADYMVRIDVEVYSRELGRSTYESRTINVSRIPPHAVPGVVDQEVSAIFRSL
                     PDR"
     gene            complement(5235..6290)
                     /locus_tag="PLM79_14760"
     CDS             complement(5235..6290)
                     /locus_tag="PLM79_14760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV87619.1"
                     /translation="MNDTTSAPEPRTNHNGFPPDAFSRLDESPDSLFYARDRFVSHLD
                     SLALSTVRRIIGRLVVEDSPTILDLMAGWDSHLPETMHPSRTVGLGLNPNELAANQRL
                     TEWVLHDLNMDPSLPFPSDTFDVVLNTVSVDYLVKPREVFAEVNRVLKPRGLFLVIFS
                     NRMFPEKATRIWKNSTEEERLRLVLDFFRTTPGFLEPRHFESSGKPRPSDDKYAHLGI
                     PSDPVFAVYAEKEGSSSGRRERPAPEQLEEEPPAACATPAERVAGEWRTCPHCGSGLK
                     KWRFPVSPFSNWDIEFVYICFNDSCPYLLRGWAVMRAQGNSGTSYRYAYEPVRKTPLV
                     LPIHTLHDLKNGIVEES"
     gene            complement(6543..6992)
                     /locus_tag="PLM79_14765"
     CDS             complement(6543..6992)
                     /locus_tag="PLM79_14765"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOV87620.1"
                     /translation="MAEEPLIERMKGLLRDNRLCVLATCHENRPHCSLMAYLCGEDAR
                     VIYLVTLANTRKFQNISLNPQVSLLVDSRTQTPGDPSRIQALTVTGTYSPLEDERLEA
                     AILDNMLEVHPHIRGLIHHPAARVLPIRVRSFQLLDGALSSHFLSVE"
     gene            complement(7111..7461)
                     /locus_tag="PLM79_14770"
     CDS             complement(7111..7461)
                     /locus_tag="PLM79_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87621.1"
                     /translation="MRKSRILIALVLAICCMFVASQVMAQTPTKGTKAAGEADQTVKP
                     DKSEQATPEMKEKREGTPGRGTKDAGEADQTVKPKEGTEAPKAPMTEKKEGTPTMGTK
                     DDPKGGPQSTAVPK"
     gene            complement(7800..8987)
                     /locus_tag="PLM79_14775"
     CDS             complement(7800..8987)
                     /locus_tag="PLM79_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOV87622.1"
                     /translation="MIKTTRDFFLDIDHYQYCKNKQFVAGDVFLMQKIREENRVIAVL
                     SDGLGSGVKANVLATLTATMAIKYISNYADVRETAEVIMDTLPVCKVRMISYSTFTIV
                     DVNSTGHARIIEHGNPEYVLLRDGEPVSVPKTPIHLERWHDREVVFSEFDLHIGDRVL
                     FFSDGVSQAGMGRPDYPIGWGRRRVIEQALKWISWQDGISSRQLAMKIALRARQIDAY
                     EPKDDISCGVMYLRHPRTLLVLTGPPFSESKDAELANLVRSFTGRIVICGGTTASIVG
                     RELDREIKMDLENLDPEIPPSSEMEGIDLITEGTLTLSKVSEILERGQKPELMRSNAA
                     TTLASILLDSDVIFFVVGTRVNEAHQDPNLPVELDIRRNIVKKITLLLEEKYLKETHK
                     RFI"
     gene            complement(8984..>9721)
                     /locus_tag="PLM79_14780"
     CDS             complement(8984..>9721)
                     /locus_tag="PLM79_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV87623.1"
                     /translation="PQSMIPRPLAAPVWKGFQPDPVPVKEYSDSIIRQTLQTLNKYGP
                     EDELNCGGCGYDNCRELAKALIDGKAEKVMCVAYMRKLAQKKTNALMEKTPSGIVLVD
                     EELQIIECNLNFAKILGADVEKAFKASPGLEGVPLREIAPFYSLFKTVLEKGEDILNR
                     DLRYKKSVFHIDIFSIEKNRVACGIFQDITKPTIHKEAMIHKAQDVIKKNLATVQQIA
                     YLLGENAAETEVILNSIIHSMTTPELE"
BASE COUNT         1723 a         2742 c         3114 g         2142 t
ORIGIN      
        1 cctggattga gcgatttggc cggcacgcat cccgtttcgt cattcccgcg gacaaacggg
       61 aatccctgaa aggcgtgcat accctccggg tgctgggtgc cggcctgcgt ggacctgacg
      121 aggggatggt ctcggaatcg ctcaaatcag ggattcgcca tgcgtgccgg atttggtcgt
      181 gaaccgatgg cgagaaccat gaagaaagaa aatacggaac ccgcttcccc ggggaacctc
      241 cgggaatctc ccggtctgaa atggatcgtc ttctccctgg tttgtgcggc gtttaccacc
      301 gtttatatca cccagcccgt actgcccgtg atccgggcag aatttcacgt gagtgagact
      361 cgggcctcct ggaccatcgc cgcggtcgtc ctgggaatgg ccctctccaa cctgcctttc
      421 gggatgctct ccgaccgata tcccctgcgg ccgatcattc tgaccggcgg cgcaacggtg
      481 gcctcggccg ggttcctttg cgccgcgact tcgagcctgg cggtgctcgt gggcgcccga
      541 tttgtccagg gcctttttct tccctcactc accacctgcc tggccgcgta cctggcccgc
      601 agtcttccgc ccgcatccct caacgtggtc atgggtacct acgtgtcggc gacggtggcc
      661 ggagggttgg ggggccgtct tctcggcggc tggattcatc ctcccctcca ctggcggttc
      721 gcattcgtga gcgcctctct catgctcctg ggggcgaccc ttgcggcggt gaagcgactg
      781 ccgggcaacg ggcccgttcg tcccgcggat acccctgccg cagggttcct tcggttgctt
      841 tcccgaaaag agtttctccg gatctacctg gtggcgtttg gagggttctt tgccttctcg
      901 tccgtcttca attatctgcc cttttatctt tcggggtccc ctttcggggc ttccaccgag
      961 ctcattacgc tactgtatct ttcctacact gtggggatgg tggtcggtcc cctggcaggt
     1021 aaggtcagca actccatcgg caacggcaac accatgatcc tgggtgccgg catcttgggc
     1081 ctttccatcg tcgcgaccct cattccgtcc ctgctgctca tcatcgtgag ccttgtggga
     1141 atctgcatcg ggtttttcgc cgtccatgcc tcggctgccg gctctctgaa ccgcaagttg
     1201 accggaaacc agggcagagc caattccctc tacgtcctgt tttattacct ggggggagcg
     1261 gtaggaatct acctgagcgg tcacgcatac gcgttgtggg gatgggccgg tgtggtcggg
     1321 gtgaccgtgt cgatgctcgt gattcctctg gcaacggggg ctgcggaaag gagagaggta
     1381 gggagagccc tgagaaaggc gggtccgttg tggatgcgtt cggtccaggg gagcgggctt
     1441 cggaagcggt agaattttac ggagtcctcg ggcaacagcc taagggccgc cacttcgccc
     1501 gggggtcccc gcaccccggg acgaagcgga gttccggcac gaaggatctg tcacgcgtcg
     1561 gtaaggttaa gaaccgggac ccggaaagcg aagttgtccc ggtaataacg gaaccggtat
     1621 tcttccggga tcttgggccg aaaggggttg gtatcgatct gttcggtctc ctccgctgcg
     1681 aggaagagag ccgggttttc cagggtccct cgggcggagg ttgttccggg aaaaggggtc
     1741 cccagggcga gactcaacgc caggaccgcc aacccgaggc ccagccttcg cagcagggca
     1801 ctcagccgtt gacggaccga ggtcttttct tcgtgggtct cgtgcactcg ggagttccgg
     1861 cacccctgaa gctttccgga tcccaggttt ctccacgggg tcgattcccg gaccgagagc
     1921 gtctttcgaa tcagtgtgct caggattccc agcatggtcg ttctccttgg ctgtaggatg
     1981 ttcgttcctt gcggaggtat cccggaggcc ctgtttcagg actgtgagac ggcaggttcc
     2041 ttcggcagat tcctcttttc aagttagaac gtgcagggtt ggtgtcatgg aaccgggcct
     2101 tcgaggggcc cggccgggtg ggagcgggtc tctcatgacc gtattcccct gggattcccg
     2161 gcccggtggg attcccggcc cggacacggc ccatggcggt cgttcaccga agactttgat
     2221 tcccgacgca caggggtccg gtttttgcct ggctcatcct tgccgggtct gatatcctga
     2281 ttgcgtccgc ccctgctctg ggtggttttc ccatagaacc cgtttccgga tcctcggggg
     2341 tccccgcttg gaaatgggcc cggcatgccg gttcagggca ttcagggacg ttcttcttcg
     2401 gagagtgctc cttccgccat gggcccgccg gacacgagcc ccaccgtggg cggcatcctt
     2461 tcgcagaaga cgtctcgaga cgggttttgc cggggaaccg gacaacgatg gacggacctc
     2521 gtgtcaggga ggaagagtga tgcaggtttt cactgcggtt tgcctgtttg cgttcacggt
     2581 gttcgctcct gccgtgtctt gggcaacttc cccccgctcg ccggatctgg gttctgcggt
     2641 gtcggggacg atcaagatcg gaggagcctt ctctctgacg gggggcggtg ctgccctgga
     2701 cctcgaggcg gccgacgggg ccaggttggc cttgacggag atcaacaggg ccgggaagac
     2761 cctgggcaag actatggaac tggtaatccg ggacagtcaa tcccgtccgg acctggcgga
     2821 acagatcgcc cgacagttgg tggagaagga tcgcgtggtc gccatcctgg gatacagcga
     2881 caccgattcc gcattggccg tagggccggt catccggaaa cacaggatcc ccttcatcac
     2941 ggtgggagcg acctccccca aacttcccga gcaggtggga gaaatgatgt tcctggcatg
     3001 tttcggcgac aacgtgcagg cggcggcggg agccgagttc gccttcaaac acctgggtgg
     3061 cggcgcttgc ctcctttggg acaaagggat ggactacact cgccttctgg ccgggtattt
     3121 caagcgccgg ttcattgagc tggggggcag cgttgtgatc gaggagccct tcgacgatga
     3181 aacgccggat ttatccgtcc agattcagca attgaaatcc ctgccccggt ccccggattt
     3241 tttttacata gccgccatgc cctacaatgc cgggatggtg attcggcagt tgcgggaggc
     3301 cgggttttcg gcccccgtcc tgggaggcga tgggtttgac acacctcaaa tcgtagagat
     3361 tgccggacct gcggccgagc gggtgttctt caccacccat gcgctggtgg accccacccg
     3421 gggaaaagag gaaatgaaga ggttcatcac ggccttcatg gcggagtatg ggcgggaacc
     3481 ccggaattcc tttgcggccc tgggatacga tgccatgcgc ctggtggtgg acgccgtccg
     3541 tcgagcgggt tccacggagc ccagctccct gaagcacgcc ctggaggaaa ccagggagtt
     3601 ttcggggctt accgggacga tcaccctgag cacaaagtcc cacattcccc aaaaatccgt
     3661 taccatcctc gagatcaagg acggcaaatt ccgctgggtg gaagatctcg ttccccagtc
     3721 ggtcccttct ccgtaggctg gaaggccggg ggccaggggc tttaggctag aggccgggaa
     3781 gcgagaaggc acgtggggcg ggctgcgctt cgctccaccc accctacctc ccggccggtc
     3841 tcgccccatg cccctgtcgt gtcctgccgc ccggcaggcc cggatctagg gcgttcagta
     3901 ctcccgggcg cccttgcccg taagccaggg ggtaacgggc aggttgaagt ggctgatggt
     3961 ggaaaccagc tcgatccagt ttttgaactc cttttcttcc cggaggatca agaccacgcc
     4021 cggcgtcttt cccgctcgca ttccgtggac gagtgccctg cccaaagcgc ggtcccaatg
     4081 ggcggcgaag gaaaactcga cggcatgagt ctccgtcacg cagtgggccc gccctccgcc
     4141 cgggagaagc acgtcggttc ggcctccgtg ttccgcacac cacgagtccc gaaagggttt
     4201 ttccggatcg acgttcttgg caaagcccgc tccctgggag aaaaggagga tgacccacag
     4261 gagcaggaag atcgatttgt atgccatggc caaagggttt ccttttcttt gaggaccggc
     4321 gtttccggag tacggttgcg ggggacgctt cgggtccttg gatggtccgt cctggttagc
     4381 gctttacaaa aaccgtcaac ggatttaatt atgaaacgtc ttattagcaa gagttgagag
     4441 caggaaagag aatggacgcc ttcattcctt aatggcagga gcagaccgat gagaatgacc
     4501 gcaacaagtc ctaattggaa ggtgttgggg ctcttggtgg tggtgtttgc cgtgggagcc
     4561 tgcgcggcga acggtccgta ctacaccgtt tcggagacgc cttcggcccc tcccccgccg
     4621 cccgcaacga ccgtcgcccc cccgcccgtg tcgacgggtc ttgcggtggg gcgcaatgaa
     4681 ttcaactaca gcttcgacct gatcgatcgg actcccttca atatcgtaga cctgccccgt
     4741 tccctggata ttctctaccg gaagggctac gacgaggttc gccagagggg acaggcggac
     4801 ctggtcgtta cggtcaccct ttccgccggg gcggcggaga atcctgagaa gagagtaggc
     4861 catgcggtgg gtggagcctt gggaggggcc gccatcggtg ccatcatcgg aggagccctg
     4921 ggagatcccg ggacgggggc cgccttgggt gcagccagcg gaggcgcagc gggggccctg
     4981 gcaccggccg ccgattacat ggtgagaatc gacgtggaag tatacagtag ggagttgggc
     5041 aggagcacct acgaaagccg gaccatcaac gtgtcccgta ttccccccca cgccgtgccg
     5101 ggggttgtgg accaggaagt ctccgccatc ttccgaagcc tgcccgatcg ttagcattct
     5161 cgtccctttc ctgcattctc ttcttatgga aaagcacggg ctctgccggg tcccgtgctt
     5221 ttcgtccttt tgggtcagga ctcctccaca attccgttct tcaggtcgtg cagggtgtga
     5281 atgggcagga ccaggggggt cttgcgcacg ggctcatagg cgtatcgata agaggtcccc
     5341 gagttccctt gtgcccgcat aacggcccag ccccggagca aataagggca cgaatcattg
     5401 aaacagatgt agacaaattc aatgtcccag ttgctgaagg gactgacggg aaacctccat
     5461 ttcttgaggc cggaaccgca gtgcgggcat gtccgccact ctcctgcaac ccgctcggcc
     5521 ggcgttgcgc acgcagcagg aggttcttcc tccagttgct ccggtgcggg ccgctctctt
     5581 ctccccgagg aagatccctc tttttccgcg tagacggcaa agacggggtc gctgggaatg
     5641 cccaggtggg cgtacttatc gtcggagggg cggggttttc cgctggattc gaaatggcga
     5701 ggctctagaa agcccggagt ggttcgaaag aaatccagga ccagacggag tcgttcctct
     5761 tcggtggaat tcttccagat ccgggtggcc ttttcgggga acatccggtt ggaaaagatc
     5821 accaggaaca gtccgcgggg cttgagaacc cggttgactt ccgcgaacac ttcccggggc
     5881 ttcacgaggt agtccacgga caccgtgttg aggaccacgt cgaaggtgtc cgaggggaac
     5941 ggcagagagg gatccatgtt gagatcgtgg aggacccatt cggtgaggcg ctggtttgcc
     6001 gccagttcgt tgggattcag ccccagtccc acggtcctcg aagggtgcat tgtttcgggg
     6061 agatgggagt cccaaccggc catgaggtcc aggatggtcg gcgaatcctc caccaccagc
     6121 cgaccgatga tcctccggac ggtggacagg gcaagggaat ccaggtggga gacgaagcgg
     6181 tcccgggcgt aaaagaggga atcgggggat tcgtcaagcc gggagaaagc gtccgggggg
     6241 aatccgttgt ggttcgtgcg gggctccggt gccgatgtcg tatcgttcat agtcatcctc
     6301 cataaaaaaa agccgagttt ctaaacccgg ctcgtggccc catttctggg caggtttgcg
     6361 tggattcttc caattttcgc tttacttaat tcctgcaggg gaaatgtcaa ggaggggagg
     6421 acgggggtcc cggtttttgg gggcctacgg gcgcaggaag ggtcgggggg gctgcacggg
     6481 agacgtccca tcgattccgg tctttggact cgatgggaag gcggaccggc gacttgcccc
     6541 ggctactcca ccgacaggaa atgagaactg agtgcgccgt cgagaagctg gaaagaccga
     6601 acgcggatgg gaaggacccg ggcggccggg tgatgaatca gcccccggat atgggggtgc
     6661 acctcgagca tgttgtcgag gatggccgct tccagacgtt catcttccag gggtgaatag
     6721 gtgccggtca cggtgagggc ctggatccgg gacgggtccc ccggggtttg ggtacggctg
     6781 tccacgagca ggctcacttg ggggttcaga gaaatgtttt gaaattttcg agtgttggct
     6841 agagttacca ggtagatcac ccgtgcgtct tccccacaga ggtaggccat gagcgaacag
     6901 tggggcctgt tttcgtgaca ggtggcaagg acgcacaagc ggttgtcccg gagaagccct
     6961 ttcattcgct cgatcaacgg ttcttcagcc atggtaccct tcttccgcgg attcaagcct
     7021 cccgcggtgt gggaccgcgc aggaagtttg ctgaagttag gcaggcctga atccaagttc
     7081 ttttcaatgg accaatgtcg gtagggcctg ttatttgggg accgctgtgg actgaggacc
     7141 cccctttgga tcgtctttcg ttcccatggt aggagtccct tccttcttct ccgtcatggg
     7201 ggctttgggc gcttcggttc cctctttggg tttcacggtc tggtctgctt ctccggcgtc
     7261 cttggtgcct cttccgggcg tgccttcgcg tttttccttc atttcgggcg tggcttgctc
     7321 ggacttgtcg ggcttgacgg tctggtctgc ttcgccggcg gctttggtgc ccttggtcgg
     7381 cgtttgggcc atgacctggc tcgctacaaa catgcagcaa atagcaagaa cgagtgcgat
     7441 cagaatcctg gacttcctca tgaaccctcc ttatcgaaca actaggcctt gcctaacttc
     7501 agccgatcag aaatgtggtg ccttgcagtc aagaagatgg tttgtgtgaa atgatatgct
     7561 cgatacaggg ggaaattcta gtgatcttgc aggaaaacac aatgaaattc tgaagggtgg
     7621 aatcaagaag ctgcaccgga cccgggtccg gccgggaaat cgcctctttc ctgtagcggc
     7681 ggggaggtgc gggtggtctg cgaccggccc cggagagggt gaaacccggg gatcggtccc
     7741 ggcggccgcg cgaaatagaa cttcgtcccc gggaccgacg gcggacggtt ccgttggtgt
     7801 cagatgaagc gcttgtgggt ttctttaaga tacttctctt ccaggagcag ggtgatcttc
     7861 ttgacgatgt tgcgccgaat atcgagctcg acggggagat tggggtcctg gtgggcttcg
     7921 ttgacccggg ttcccaccac gaagaagatg acgtcgctgt ccaacaggat cgaagcgagg
     7981 gtcgtggcgg cgttggaccg catgagctcg ggtttttgtc cgcgttccag gatttcggaa
     8041 accttgctca gggtcagggt gccttcggtg atgaggtcga ttccttccat ttccgaagag
     8101 ggggggatct cgggatccag gttctcgagg tccattttga tctcccggtc cagttcccgt
     8161 ccgacgatgg aagcggtggt cccgccgcaa atgacgatcc gtccggtgaa ggaacgcacc
     8221 aggttggcca gttcggcgtc cttggattcg gaaaacgggg gacccgtaag gaccaagagg
     8281 gttcgtgggt gtcgcaggta catgaccccg caggagatgt cgtccttggg ttcataggca
     8341 tcgatttgtc gggcgcggag ggcaatcttc atggccagtt gacgggagga gatcccgtcc
     8401 tgccaactga tccacttgag ggcctgttcg atgacccgcc ttcgtcccca gccgatagga
     8461 taatccggcc tccccatgcc cgcctgactg acgccgtcgg agaagaaaag gacccggtct
     8521 cctatgtgca ggtcgaactc ggaaaaaacc acctcccggt cgtgccacct ctcgagatgg
     8581 atgggggtct tggggacgga gacgggttct ccgtccctga gcagcacgta ttccgggttg
     8641 ccgtgctcga tgatgcgggc gtgcccggtg ctgttgacgt ccacgatggt gaaggtggaa
     8701 tagctgatca tgcgcacttt gcataccgga agggtgtcca tgatcacctc ggccgtctcc
     8761 cgcacatcgg cgtaattcga aatatacttg atggccatgg tggccgtgag ggtagccagg
     8821 acattggcct tgacgccgct gcccaggccg tccgagagta cggcgatgac ccggttctcc
     8881 tcccggatct tctgcatgag gaagacgtct cccgccacga attgcttgtt cttgcaatac
     8941 tggtaatggt cgatgtccag gaagaaatcc ctcgtggtct tgatcattcc agctccggcg
     9001 ttgtcatgga atggatgatg gagttcaaaa tcacctcggt ttcggcggca ttctcaccca
     9061 acaggtaagc gatttgttga actgtggcca agttcttctt gatgacgtcc tgagctttgt
     9121 gaatcatggc ctctttgtga atcgtgggct tggtgatgtc ctggaagatc ccgcaggcca
     9181 cccggttttt ctcaatggaa aagatgtcga tgtggaagac cgacttcttg tagcgcaagt
     9241 cgcggttgag gatgtcttct cccttttcca ggacggtctt gaagagggaa taaaaggggg
     9301 cgatctcccg cagaggcact ccttccaggc cgggggaggc cttgaaggcc ttttccacgt
     9361 cggctcccag gatcttggcg aaattgagat tgcattcgat gatctggagt tcctcatcca
     9421 ccagcacgat gccgctgggg gtcttttcca tgagggcatt ggtcttcttc tgggccagct
     9481 ttcgcatgta ggccacgcac atgacctttt cggccttgcc gtcgatgagg gccttggcaa
     9541 gctcacggca gttgtcgtag ccgcagcctc cgcagttgag ttcgtcttcc gggccgtact
     9601 tgttgagggt ctggagggtc tggcgaatga tgctgtcgct gtattccttc acgggcaccg
     9661 ggtccggttg aaagcctttc caaacggggg cggctagagg gcgtgggatc atggactggg
     9721 g
//
LOCUS       DAOOVC010000146         9681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4453_length_9681_cov_6.338251, whole genome shotgun sequence.
ACCESSION   DAOOVC010000146 DAOOVC010000000
VERSION     DAOOVC010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4453_length_9681_cov_6.338251"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..417
                     /locus_tag="PLM79_14785"
     CDS             208..417
                     /locus_tag="PLM79_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87624.1"
                     /translation="MEPGKELIVAARVRLAAKLPKRYDGLCDFRLTARLRTMESHGDA
                     VEKRVPIVGPKKASMGREDGEGFHS"
     gene            complement(576..1004)
                     /gene="tnpA"
                     /locus_tag="PLM79_14790"
     CDS             complement(576..1004)
                     /gene="tnpA"
                     /locus_tag="PLM79_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOV87625.1"
                     /translation="MSRFRKLSHTIWHCQYHIVWVPKYRYRVLIGPVRDAAHSGIQAI
                     CGYAGCEVVELNVQRDHVHMIVMIPPKVSISDLMGRVKGQTSIKLFKQFHQLRKKPYW
                     GNHFWARGYCVDTVGIDADMIRKYVRYQEKKERTEEQLTF"
     gene            1458..1706
                     /locus_tag="PLM79_14795"
     CDS             1458..1706
                     /locus_tag="PLM79_14795"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOV87626.1"
                     /translation="MPVVKASSKGQIVIPKELREKLGIGAGRKLLLRLVGNHMEIVPL
                     PDEPVKALRGMLKAETSLAAELLEERRRDDACDEKRHV"
     gene            1687..2085
                     /locus_tag="PLM79_14800"
     CDS             1687..2085
                     /locus_tag="PLM79_14800"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOV87627.1"
                     /translation="MKSVTFDSHAILKFAQDERGADRVEELLRAAEKAQVRAYVNEIN
                     LGEVYSITMRRLGAESARRFLDQFSTLTVERVAATWEVIESAAELKARHALSYADCFA
                     AATALKHGASIVTGDPEFKKVEHLVPIEWI"
     gene            complement(2280..2357)
                     /locus_tag="PLM79_14805"
     tRNA            complement(2280..2357)
                     /locus_tag="PLM79_14805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2320..2322),aa:Pro,seq:ggg)
     gene            complement(2435..>3479)
                     /locus_tag="PLM79_14810"
     CDS             complement(2435..>3479)
                     /locus_tag="PLM79_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV87628.1"
                     /translation="VQNGLEFTRLKEENRLWRQKIQRRCRMTGDSTVVQSLRDQIRRA
                     APSNATVLISGENGTGKELVARMIHSLSRRNRLPMIEVNCAAIPEELIESELFGHEKG
                     SFTGAYERRKGKFDQANGGTLFLDEIGDMSLKTQAKILRIIQEQVFERVGGSRPIQVD
                     VRIVAATNKDLTREIEEGTFRQDLYYRLNVIPIRVPPLRERIEDIPLLVEDFMTEMAH
                     ESTMGRKKLDPEVVRVLQQYSWPGNVRELRNFVERLLIMSPGQIIRAEDLPSDFTGRL
                     PNPPANHDPYHCTTLKEARCRFERQFLLQKLKLYDWNVSLTAQQIGLERSHLHRKMRF
                     LGIREAEDAPGSS"
     assembly_gap    3480..3489
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3490..3829)
                     /locus_tag="PLM79_14815"
     CDS             complement(<3490..3829)
                     /locus_tag="PLM79_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87629.1"
                     /translation="MKPRILVVDDEISILNSLRGVLQDEGYLVSTAASGEEALEEVRK
                     ELPDLVILDIWMPRMDGLDVLEAMKGLAPSLPVIIISGHANIETAVKATRMGAFDFVE
                     KPLSLERILVS"
     gene            complement(3855..6026)
                     /locus_tag="PLM79_14820"
     CDS             complement(3855..6026)
                     /locus_tag="PLM79_14820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87630.1"
                     /translation="MTLIFLLTLFFGVLEGWFFKLQPDLPLVGNIFLFALIHLNVILL
                     LLLVYLVLRNLVKLLFERKRNVLGHKLRTRLIVAFVGLAVIPTIPLFWLATQFIFSSL
                     DYWFSQKVEQSLEQAVGLAKEYLDQEGRDLLYDAQVAKGELRGILSEKGSVSSFPGRV
                     PLSLLDFHHLDAMFHFDSAGNLVWTLHRKSVPSDVSDMVQQMSRSDLPKKPQVQRVSL
                     DSRGQRLEGLVALVPDPIGDPDAPGSAELLVCLRFFPDSIAGRLAAITAGYEDYLQLK
                     LLHKPLKTSHFVTFSIVTLLVIFAAIWFAFLLARNITGPIQSLVYATQRIADGDLDVQ
                     MDSDRQDEIGMLMSSFDQMVRDLKEGREKLANAYWELKETNQELDGRRRYMEAVLKNI
                     AAGVVGVDAGGTIRAVNKSAEDTFGLKAEEVRGQHYTRFLQPDHMEIIKSFVSSFRLS
                     GQPYQERRVRVVMGNRPMVLFVKVSTLRDEQDEFMGIVIVLDDLTELEKAQRMAAWRE
                     VARRIAHEIKNPLTPIRLSAQRLRRKYAEWIEEEGSVLDECTQAIINQVDHLKHLVNE
                     FSTFARLPSVQLVPCDLVPIVEESIALYRNNYTQVTFLLEIATPVPQLQLDPEQFKRV
                     LINLLDNAIHAVKDRAGVISIQLFYDPILKIARLECADNGHGLSAEDKVRMFEPYYST
                     KEKGTGLGLAIVASIVADHNGYVRVRDNTPEGTVIIIELPG"
     gene            complement(6098..6673)
                     /locus_tag="PLM79_14825"
     CDS             complement(6098..6673)
                     /locus_tag="PLM79_14825"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOV87631.1"
                     /translation="MTISFICRLSLLFLSGFVSLLPCSAAAAGRAEIVEVRVTPRAEN
                     LELGLRIQNCFTPKMEEAILSGVLTTFRIRIVLERSGPSFLRARLLDTTLDRTVKYEP
                     LGGEFRVKLPEQPDRVLITKDFHEAKGWMSRIRDLPIISLDSLQRGVKYELRIKAELS
                     KFHLPLFFRYIFFFVSMWDFETDWHKVPFSF"
     gene            complement(6827..7774)
                     /gene="lpxC"
                     /locus_tag="PLM79_14830"
     CDS             complement(6827..7774)
                     /gene="lpxC"
                     /locus_tag="PLM79_14830"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOV87632.1"
                     /translation="MAKSNSKQHTIGREVWCEGVGLHSGANVALKLYPAPPNFGIRFK
                     RYDTPGQPPIVAHPWRVVDTVLATSIGVNGVVVATIEHLMAALMGCGVDNVLVEVGGS
                     EIPIVDGSAAPYVDLLTEAGRRAQNASRPCIRVTRPFVVKEGDAYIKALPAQRMELHY
                     VIDFPHPLVGRQEMSWTFDPASFRRDIAQARTFGFLKDVQRLQRMGLALGGSLSNAVV
                     FDEEGVLNREGFRFSDECVRHKILDFLGDLALGGVGLVGRFEAYKAGHSLHNRFIKEL
                     ITREAAARETIPAMLAPPFMGPASIPGLMDPFPGLGKPL"
     gene            8113..9615
                     /gene="thrC"
                     /locus_tag="PLM79_14835"
     CDS             8113..9615
                     /gene="thrC"
                     /locus_tag="PLM79_14835"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOV87633.1"
                     /translation="MKPDQFPPEIQSYLVPSPAGLYLYECLGCGNTFDVGRLLYTCPD
                     CKSVLMIRDREWERLKAVPGDLWRKIFDYRSMLTIPALRGIYRFHELLGSVLPLDAVV
                     YLGEGHTPVIASNERLCDWTGLPVFFKNDGQNPSASFKDRGMASAFSYLNFLLKAGSL
                     TDVLAVCASTGDTSASAALYASYLGDAVKSAVLLPHGKVTPQQLGQPLGSGARVIEIP
                     GVFDDCMKVVEFLSENFPVALMNSKNPWRILGQESYSYEIAQAFDYEVGNLAVAVPIG
                     NAGNITAVLQGFLKLLDLGIISSLPWVVGVQSAHANPVFLYYLETDPRKRSYRPVRVQ
                     PSVAQAAMIGNPVSMPRVMRLVERYRDLAGYEAFQAVEVSEQEIMDAMLWANRNGHVA
                     CTQGGECLAGIRKAVAGGSVPRDRAAVLDATAHALKFAVFQEKYFSDSFEPEFSISPR
                     PELQNAPTCIPVPEDLPSPLSRKDLSPSELKAYVEYAAGKVAGILGLESE"
BASE COUNT         2034 a         2833 c         2853 g         1951 t
ORIGIN      
        1 cccccttcaa ggccggccac gatttccagc ccagacagag ataggtcgcc ctgggagact
       61 cgaaggagag ccggtggttc aaggggcgag cggcatccgg cgacggagtc gcccggtatc
      121 gagcactgct gatcggcatc tccccttacg aggacaaagg acatctcgtc ttcactgcgc
      181 aggaaggggg gatgggtaac cccggagatg gagccgggca aggaactgat cgtcgcggca
      241 cgtgtccggc tggccgcaaa gctccccaaa agatacgacg gattatgcga tttccggctc
      301 acggcgcggc tgagaaccat ggagtcccat ggggatgccg tggaaaaacg ggtcccgatt
      361 gtggggccga aaaaggcatc catgggaagg gaggacggcg agggattcca ctcctgacgg
      421 attcgcaaaa agccttcacg tagccgtttc gactctataa actagtaaaa aaccccaccc
      481 aaagggtggg gctttaaatt caccccccgt aggggggcaa agccaacccc cagagggggc
      541 tggcctaagc cgtgccgcct ttgagtttgt aggtttcaga aggtcaactg ctcttcagtt
      601 cgttcctttt tttcttgata ccgcacatac ttgcggatca tgtctgcgtc gataccaacg
      661 gtgtcgacgc agtagcctct agcccagaag tgatttcccc agtatggctt ctttcggagc
      721 tgatggaact gtttgaagag cttgatcgat gtctgacctt tcactcggcc catcaagtcc
      781 gatatggaaa ccttgggcgg gatcatgaca atcatatgga catgatccct ttgtacgtta
      841 agttcgacga cttcacatcc agcgtagccg catatcgcct ggatccccga atgtgcggca
      901 tcccttactg ggccaataag aacccgatac cgatacttcg gaacccatac gatgtggtac
      961 tgacaatgcc agatcgtatg tgataacttt cggaacctgc tcactgctcc ctccttcttc
     1021 tcgatgttgc ggcaacaact cgagttgagt gatagcagtg agtgggcctc tggccaaacc
     1081 cattcgggac cggcatagcc agtcccacat gaccacgccc aaagggcgtg gatttctacg
     1141 ccgcattaaa attccgaggt tcggttttga ggaaacgact tttacgcgat catcttccct
     1201 gttctgacag gggcaggaac ggtgaaaacc aatgcaccgt tccttaccct cagaaaagtg
     1261 cgaaaggagg catgcagctt cgtgtgtgcg tcgtttcgag tgatccgcca ctcgatctcg
     1321 aatgacgcat caacctgaaa aatgttcggg cgtcattttc cgcgcgggat ggagcaggtg
     1381 ctttaccacc accacaccca tgatctgtta gactcaatcc agaaacgtaa gcgtaaataa
     1441 aaggaggtaa gagaaagatg ccggtggtaa aagcatcatc caaggggcag atcgtcattc
     1501 ccaaagagct tcgggaaaaa ctggggatag gggccggcag gaagctgctg ctccgcctcg
     1561 tgggaaacca tatggagatc gttccgctgc cggatgagcc ggtgaaggcc ttgcgcggaa
     1621 tgctcaaggc ggaaacttcc ctggcggcgg agttgcttga ggaaagaagg agggacgacg
     1681 cctgcgatga aaagcgtcac gtttgattcc catgccattt tgaagtttgc ccaggacgaa
     1741 aggggagcag accgggtgga agaactcctg cgcgcagccg aaaaggcgca agtgcgcgcc
     1801 tacgtgaacg aaatcaacct gggagaagtc tattccatca ccatgagaag actcggcgcc
     1861 gagtcggcac ggcgatttct ggaccagttt tccaccctga ccgtggaaag ggttgccgcc
     1921 acctgggaag ttatcgagtc cgctgctgag ttgaaggccc gtcatgccct ttcctacgcc
     1981 gattgcttcg ccgccgccac ggcgctaaaa cacggggcct ccattgtgac cggagacccc
     2041 gaattcaaga aggttgagca cttggtccct attgaatgga tatgagccgg acgggtcagg
     2101 aaacgagggc actgagtcaa gaatgatccc atccgtttgg ccaaacaggc cgacgaggca
     2161 tcgaggattt cctcaagacc actctcccct cttgttttcc ccctctttgc cgtcttccaa
     2221 aaagaaaagg ccccttgcgg gcaaggagcc gattttcttc ggggatttct tgtgttgtgt
     2281 ggtcgggacg agaggatttg aacctccgtc ctcttgcacc ccaagcaagc gcgctaacca
     2341 ggctgcgcca cgtcccgaca cagggggtag ttatcactga tccccatacc gtgtcaaggt
     2401 gcattgtggg tccgggatgc cctccggcgg gagctcagga gctgccgggg gcgtcttcgg
     2461 cctcccgaat gcccagaaac cgcatcttgc gatggaggtg gctgcgctcg agcccgatct
     2521 gctgggccgt gagggagacg ttccaatcgt agagtttgag cttctggaga agaaactgcc
     2581 gctcgaaccg gcaccgcgcc tccttcaggg tggtgcaatg gtaggggtcg tggtttgcgg
     2641 gcgggttcgg cagccgaccc gtgaaatccg aggggaggtc ttcggcccgg attatctggc
     2701 cgggggacat gatgagaagc cgctcgacga aattcctcag ctcccggaca ttccccggcc
     2761 aggagtattg ctggagtacc cgaaccacct cggggtccag tttcttgcgg cccatggtgc
     2821 tttcgtgggc catctccgtc atgaagtcct ccaccaagag cgggatgtcc tcgatcctct
     2881 cccggagcgg gggcacccgg atcgggatga cgttgaggcg gtagtagagg tcctgtcgga
     2941 aggttccctc ctcgatctcc cgggtcaggt ccttgttggt ggcggccacg atgcgaacgt
     3001 ccacctggat gggccgggag cctcccaccc gttcgaacac ctgttcctgg atgatcctga
     3061 ggatcttggc ctgggtcttg aggctcatgt ccccgatttc gtccagaaac aaggtgcccc
     3121 cgttggcctg gtcgaacttg cccttccgtc gttcgtaggc cccggtgaaa gacccctttt
     3181 cgtggccgaa gagttcgctt tcgatcagct cctcggggat ggccgcgcag ttgacctcga
     3241 tcatgggaag ccggttgcgg cggctcaggg aatgaatcat ccgggccacc aactctttcc
     3301 ccgtcccgtt ttccccgctg atcagcaccg tagcgttgct gggggcggcc ctccggatct
     3361 ggtcccgaag ggactgcacc acagtactgt ctcccgtcat ccggcaacgc ctctggatct
     3421 tctgccgcca gaggcggttc tcctccttga ggcgggtgaa ctcgaggccg ttttgcaccn
     3481 nnnnnnnnnc agagaccaga attctttcca gggaaagcgg cttttccacg aaatcgaagg
     3541 cccccatccg ggtggctttc actgccgtct cgatgttggc gtggcccgaa atgatgatga
     3601 ccggaagaga aggggccagg cctttcattg cttccagcac gtccaggccg tccatccggg
     3661 gcatccagat gtccaggatc accaggtccg gcaattcctt gcggacctcc tcgagggctt
     3721 cttcccccga tgccgccgtg gatacgagat agccctcgtc ctggagcacc ccgcgcaggg
     3781 aattcaggat gctgatttcg tcgtccacca caagaatcct cggtttcatc gcaccgctgt
     3841 cgcgacctcc ttctctaacc cggaagttcg atgatgatca cggttccctc gggggtgttg
     3901 tcccggaccc gcacgtagcc gttgtgatcc gccacaatgc tggccacgat ggccaggccc
     3961 aggccggtac ccttctcctt ggtcgagtaa tacggctcga acatgcgcac cttgtcctcg
     4021 gcggagaggc cgtggccgtt gtcggcgcat tccagccggg caatcttcag aatgggatca
     4081 tagaagagct gaatgctgat gaccccggcc cgatccttca ccgcgtgaat ggcgttgtcc
     4141 agcagattga tgaggacccg cttgaattgt tccgggtcca actggagctg gggaacaggg
     4201 gtggcgatct cgagtaggaa agtgacctgg gtgtagttgt tccggtacag ggcgatgctc
     4261 tcctccacga tgggaaccag gtcgcacgga accagttgca cactgggaag gcgggcgaag
     4321 gtggagaatt cgttgaccag gtgcttcagg tggtccacct ggttgatgat ggcctgggtg
     4381 cattcgtcca ggacggaacc ctcctcctcg atccattccg catacttgcg ccggagtcgc
     4441 tgggcgctca aacggatggg cgtgagcggg ttcttgattt cgtgggcgat ccggcgggcc
     4501 acctcccgcc atgccgccat ccgctgggcc ttttccagtt ccgtcaggtc gtccagaacg
     4561 atgacgatcc ccatgaattc atcctgctcg tctcgaaggg tggatacctt cacaaagagc
     4621 accatggggc ggttgcccat gaccacgcga acccggcgtt cctggtacgg ctgaccgctg
     4681 aggcgaaacg agctcacaaa ggatttgatg atctccatat gatccggctg aagaaatcgc
     4741 gtgtaatgct ggccgcgaac ctcctcggcc ttcaaaccga aagtgtcctc ggcggatttg
     4801 tttacggcgc gaatcgttcc cccggcatcc acccccacga ctccagccgc aatgtttttc
     4861 aaaaccgcct ccatgtaacg gcgacgccca tccagttcct ggttggtttc cttgagctcc
     4921 cagtaggcgt tggccagctt ttcccgccct tccttgaggt cccggaccat ctggtcaaag
     4981 gaactcatga gcatgccgat ttcgtcctgg cggtccgagt ccatctgaac gtccaggtcc
     5041 ccgtccgcaa ttcgttgagt ggcatacacc aatgattgaa tgggtcccgt aatgttccgg
     5101 gcaagaagga aagcaaacca gatggcggcg aaaatcacca gcagggtgac aatggaaaag
     5161 gtgacgaaat gagaagtctt gagcggcttg tgaagcagct tcagttgaag gtaatcctcg
     5221 tagcccgccg tgatggccgc cagccttccg gcgatggagt cggggaaaaa ccgaaggcag
     5281 accaggagtt cggcgcttcc cggggcgtcc gggtctccga tggggtcggg gaccaaggcc
     5341 accagtccct cgagcctctg accccggctg tccagggaga ccctctgaac ctgcggtttc
     5401 ttgggaaggt cgcttcggga catctgttgg accatgtcgg aaacgtccga aggcacgctc
     5461 ttccgatgaa gcgtccatac caggttgccc gccgaatcga agtgaaacat ggcgtccagg
     5521 tggtgaaaat ccagcagaga cagggggaca cgcccgggaa aggaagaaac cgagcccttc
     5581 tccgaaagga tgccccgaag ttcgcccttg gccacctggg cgtcataaag caagtccctg
     5641 ccttcttggt ccaggtattc cttggccagc cccacggcct gttccaaaga ttgttccacc
     5701 ttttgactga accagtagtc caggctcgag aagatgaact gggtcgccag ccagaaaaga
     5761 gggatggtgg gaatcacggc caacccgacg aacgcaacga tgagtcgcgt tcgcagcttg
     5821 tgacccagca cattcctttt acgctcgaag agcagcttga cgaggttgcg caggaccagg
     5881 tagacgagaa ggagcaacag gatgacgttc agatgaatca gggcaaaaag gaagatgttt
     5941 cccacgaggg gaagatccgg ctggagcttg aagaaccacc cttccaggac cccgaagaag
     6001 agggtgagca aaaagatgag agtcacccag gtgcgttcgc gctgtcgtcg cctttgtcgg
     6061 agcaggtcct cgggaaggta cattggacaa cggagtgcta gaatgaaaag ggcactttgt
     6121 gccagtcggt ctcaaaatcc cacatagaca cgaagaaaaa aatatatcgg aaaaataggg
     6181 gtagatgaaa tttcgagagc tcggccttga ttctcagctc gtacttgaca cccctctgaa
     6241 ggctgtccag cgagatgatg ggaaggtccc gaattcggct catccaccct ttggcttcgt
     6301 ggaagtcttt ggtaatgagc acccgatccg gttgttccgg gagtttcacc cggaattcgc
     6361 cgcccagggg ctcgtacttg accgtgcgat ccagggtcgt gtccaacagc ctggcgcgca
     6421 gaaaggaagg cccggacctc tccagcacga tccggattcg gaacgtcgtc agcaccccgc
     6481 tcagaatcgc ctcttccatc ttgggcgtaa agcagttctg gatgcgaagc cccagttcga
     6541 ggttctctgc ccggggcgtc acgcgaacct ccacgatttc cgccctgccc gccgccgctg
     6601 cggaacaggg caataaggat acaaaccccg agagaaagag gagggagagg cggcaaataa
     6661 aggatattgt cattcttttc agctagttat ggaataggat cgttcactcg aaccgattct
     6721 aacgaaccgg gcggggaatg acaagggctt tatgggggag agggaaggaa gggaaaccgc
     6781 ggcccgagcc gtgccgccca gggccgcggt cccccggaag aattcgttac agaggtttcc
     6841 ccagaccggg aaaggggtcc atgagcccgg ggatggaggc cggacccata aacggaggag
     6901 ccaacatcgc gggaatcgtc tctcgagcgg ccgcctcccg agtgatgagt tccttgataa
     6961 accggttgtg aagggagtgg cccgccttgt aggcttcgaa cctgccgacc agtcctacgc
     7021 cgcccaaagc cagatcgccg agaaaatcca ggatcttgtg gcggacgcac tcgtccgaga
     7081 agcgaaatcc ttcccggttg agcacccctt cttcgtcgaa cacgactgca ttggaaagag
     7141 acccgcccaa tgccagcccc atgcgctgga gcctttgcac gtccttgaga aacccgaagg
     7201 tgcgcgcctg ggcaatatca cgacggaagc tcgccggatc gaaggtccag ctcatctcct
     7261 gccttcccac caaggggtga ggaaagtcga tgacgtagtg cagttccatt ctttgagccg
     7321 gtaaggcctt gatataggcg tcgccctctt tgaccacgaa gggacgagtc acccggatgc
     7381 aggggcgcga cgcgttttgc gcccggcgtc ccgcttccgt caagagatcc acgtagggag
     7441 cggcgctccc gtccacgatg gggatttccg atccgccgac ctccacgagc acgttgtcca
     7501 cgccgcaacc catcaaggcc gccatgaggt gctcgatggt ggcaaccact accccgttga
     7561 ccccgatgct ggtggcaagg accgtgtcca caacccgcca ggggtgagcc acgatgggag
     7621 gctgtccggg agtgtcgtaa cgcttgaagc ggatcccgaa gttcgggggc gccgggtaaa
     7681 gcttcaacgc cacgttagcc cccgagtgca ggccaactcc ttcgcaccac acttccctgc
     7741 cgatggtatg ctgtttcgag tttgattttg ccaagcccct tttcctcctc ggcggctcct
     7801 aagggcaaca ttcgtgccac aacccatggc gctgcatttg gatcggggtc tttcagagcc
     7861 gcaagggttg cggcctgtat ttactaagaa tcccgaccgg ggtcaagaat cgaatggcaa
     7921 aacgggccgg tcccgcttag agtggcataa aatccacaca ggaagaggat ctgtgttgca
     7981 tttatgccac accccaattg agtcttgatc ccgagggtcc aaaccgatta tgtacacctt
     8041 tgattccaga accgatgccg accgaggctt cccgtcgggg aagcctccct cgatccaagg
     8101 gagccgacgt ccatgaaacc agaccagttt cccccggaaa tccagtcgta tctcgttccg
     8161 tccccggccg gcctgtacct ctatgaatgc cttggctgcg ggaatacctt cgatgtgggg
     8221 cggttgctct acacctgccc ggactgcaaa agcgtcctga tgatccggga ccgggaatgg
     8281 gagaggctga aggccgtccc gggagacctc tggcgcaaga tcttcgacta tcgcagcatg
     8341 ctgacgatcc ccgccctgag agggatctac cggttccacg aacttctggg ttccgttctc
     8401 cccctggatg ccgtggtata cttgggggag gggcacaccc cggtcatcgc cagcaacgaa
     8461 cgcctctgcg actggaccgg gcttcccgtt ttcttcaaga acgacggcca gaaccccagc
     8521 gcgtccttca aggaccgggg catggccagc gctttcagct atctcaattt cctgttgaag
     8581 gcgggaagtc tgacggacgt gctggccgtt tgcgcctcca cgggggacac ctcggcttct
     8641 gccgccttgt acgcttccta tctgggggat gccgtaaaat cggcggtgct cctgccccac
     8701 gggaaggtca ctccccagca gttgggacag cccctgggca gcggggcccg ggtgatcgag
     8761 attccgggcg tcttcgacga ctgcatgaaa gtggtggaat ttctctcgga aaacttcccc
     8821 gtggccctga tgaattcgaa gaacccatgg cggattctgg ggcaggaatc ctacagctac
     8881 gaaatcgccc aggcgttcga ctacgaggtg ggaaacctgg cggtggcggt ccccatcggc
     8941 aacgcgggaa acatcacggc ggttctccag ggctttttga agctcctcga cctggggatc
     9001 atttcgtctc ttccgtgggt cgtcggggtc cagtcggctc acgccaaccc ggtcttcctg
     9061 tactacctcg aaaccgatcc ccggaaacgg tcctaccgtc ccgtgagggt tcagcccagc
     9121 gtggcccagg ccgccatgat cggaaatccc gtctccatgc ctcgggtcat gcgcctggtg
     9181 gaacgatacc gggacctggc gggctacgaa gcctttcaag ccgtcgaggt atcggagcag
     9241 gagatcatgg acgccatgct gtgggccaat cgcaacggtc atgtggcctg cacccaggga
     9301 ggagaatgcc tggccgggat tcgcaaagcc gtggcgggag gatccgtccc ccgggaccgc
     9361 gctgcggttc tggacgccac ggcccacgct ctaaagttcg ccgttttcca agagaagtac
     9421 ttttcggact ccttcgagcc ggaattttcc atcagccctc gccccgagct tcaaaacgct
     9481 cccacctgca ttcccgtgcc cgaggacctt cccagtccgt tgagccgaaa ggacttgagt
     9541 ccgtcggagc tcaaggcgta cgtggaatac gcagcgggga aagtcgccgg gatcctgggc
     9601 ctcgagtccg aatgacccgc atcgcaacaa gccgggaaag gagaatcgtc caatgaacgg
     9661 cattttgccc tacaataata a
//
LOCUS       DAOOVC010000147         9634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4470_length_9634_cov_5.959286, whole genome shotgun sequence.
ACCESSION   DAOOVC010000147 DAOOVC010000000
VERSION     DAOOVC010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9634
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4470_length_9634_cov_5.959286"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLM79_14840"
     CDS             complement(<1..476)
                     /locus_tag="PLM79_14840"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOV87634.1"
                     /translation="MDVCGTLPSPSSPNDPGNGSPRSSAPASGKAALGVDPFSCLPLR
                     LVLLLVLLAGQAVYAHAREEKEKSPSPPQVRLIQFKGVRSLSTGELKKIFTTKEKRFK
                     FFGKAPLDEEVLGKDLDLLVKYYQSRGFYHARVVSHKVLPLVGKDVRVEIQIEEGPP"
     gene            complement(534..1340)
                     /gene="ybgF"
                     /locus_tag="PLM79_14845"
     CDS             complement(534..1340)
                     /gene="ybgF"
                     /locus_tag="PLM79_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HOV87635.1"
                     /translation="MDASNRRDLRAGVILIMIGTLFGCASTQETSTMQQSIGMLHERV
                     QNLENRVRSAEGQGGKSADLYARLEELQLKVGALNGRIEELDRRIDRMSKSQASVSSA
                     PPVSATPAPSLSAQEPSAPPDAPKSSAPAQPVITIPEKAEKEPTEKGLYDKALDLFHQ
                     GRYDGARKEFQAFLNKYPKSELADNALYTIGECYYSEKRYQDAIATFQQVLDRYPKGN
                     KVPHTLLKQGAAFQALGDSTAARILYERLLEKYPDTPQAQAAEKKLKQLR"
     gene            complement(1599..2162)
                     /gene="pal"
                     /locus_tag="PLM79_14850"
     CDS             complement(1599..2162)
                     /gene="pal"
                     /locus_tag="PLM79_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HOV87636.1"
                     /translation="MRRTRGMTAVLVGFFLVAGTIGCAKKVGPQPGAVPGAGPGGAGV
                     GTGAGMGPDDARWRELGLNSEPERREFLQRAQSFENEDVYFDFDSYVLSDPAKRVLDT
                     KVEFLKRYPKVKVTVEGHCDERGTNEYNLALGERRANSAYQYLVNSGVNAQRLGSVSY
                     GEERPIAPGHDEASWAKNRRAHFVLNY"
     gene            complement(2359..3669)
                     /gene="tolB"
                     /locus_tag="PLM79_14855"
     CDS             complement(2359..3669)
                     /gene="tolB"
                     /locus_tag="PLM79_14855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOV87637.1"
                     /translation="MKYVRLLTAVLMVLLWTDSGRAQRVYIDITQPSFAPISIAVPDF
                     KFLSSEQVQLSKEMSGALAGDLEFSGIFRPLDPKGFLEDPQTMGLNAAEIRFNTWQSL
                     QAEFLVRGAYQVQGNSIRLEARLFDVFGSKMILGKVYEGDARNWRAMIHRFADEILLL
                     LTGERGVFDTKITFVQSQGKAKEIFVSDFDGGNAVQVTHDQNLNLSPTWSPDGTQIAY
                     VSFKDGNTKIYAANLLDGTQRLLSGHPGLNIAPAWRPGSRDLAATLSRGGNPDIYLIS
                     SSGDILQKLVQGWSINVSPSWSPDGRKLAYVSNETGTPQIYVLDVGSGQKRRITFSGN
                     YNTSPAWSPKGDWIAYSGSVGGRHNIFIIKPDGSDVRQLTRGEGDNESPTWSPDGRMI
                     AFSSTRQGGSAIWMLLTNGAGTRRVTRMPGSQELPDWSPRLGGR"
     gene            complement(3725..4657)
                     /locus_tag="PLM79_14860"
     CDS             complement(3725..4657)
                     /locus_tag="PLM79_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOV87638.1"
                     /translation="MEALSLSPRRLSREEVLAGMGGSVLGHVLIFGAALLLPSMMPRS
                     TTPLQFTTVNLVSMDQLGGGGAVAPPGSKSLKSEGPKESSASKSAASSQRGKSSPVVP
                     VKRLRMEESAPRDHAIKKLEAPQIAKAPENRQASAAIDKDLDKLIPKAKTSSPASSAD
                     QADRKPAQSEQSAGADGARGVDRSREKGTSTSPAGSSKGSGSGGVGANREGGQVALAR
                     RLYYTEIWNAIRRQWTLPESLKSQQLEAVLVLVVRRDGKVMNIRFEKQSGQPLFDESV
                     MRAVRKADPLPAFPEIYSPPQEEIGLRFRPEDLA"
     gene            complement(4705..5160)
                     /gene="tolR"
                     /locus_tag="PLM79_14865"
     CDS             complement(4705..5160)
                     /gene="tolR"
                     /locus_tag="PLM79_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOV87639.1"
                     /translation="MAMQTNGNGSRGQLVSEINVTPFVDVMLVLLIIFMVTAPMMTQG
                     IDVNLPESSAPAIPTEEERLMVTITKDQQVYINEYSVDLDALGAKLAALYQNLERRKG
                     VFLRADESIPYGFVVQVMGTIRQAGIDQIGMVTEPLKSPPAKDPGSRGK"
     gene            complement(5355..6161)
                     /gene="tolQ"
                     /locus_tag="PLM79_14870"
     CDS             complement(5355..6161)
                     /gene="tolQ"
                     /locus_tag="PLM79_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOV87640.1"
                     /translation="MVNSLILTAYAAAEVVAQPYQRAGNGVWDMVVHAGPMVKFVMLS
                     LLGLSVACWCVILLKYRLFRRARKETDRFVDYFRQRKNYSLLYKDSQLLEDSHLAQIF
                     QTGYLELNRLSKSLETKNLQELKVNPEVVLESVDRAIQGATMAERQRFERYLPLLATT
                     GSTAPFIGLFGTVWGIMTSFQEIGLKGAANLAVVAPGISEALIATAMGLAAAIPAVVG
                     YNHFATRIRMIDHEMSHFSADFLNILKRDLMRKGKQEESSAEPQRLAVQE"
     gene            complement(6151..6924)
                     /locus_tag="PLM79_14875"
     CDS             complement(6151..6924)
                     /locus_tag="PLM79_14875"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOV87641.1"
                     /translation="MDPRYPQIAERIASSRYVIALTGAGISVESGIPDFRSEDGLWSR
                     YDPLEYGHIDSFRMNPAKVWRMLLEMDLLLSRANPNSAHYALARMESSGYLKEIITQN
                     VDSLHQRAGSVRVIEFHGNGRELRCDGCGRSFDRGALTLETVPPVCPCGGSLRPNFVF
                     FGEMIPPSVLEEARLAARSCDMMLIVGTSATVAPASHLPHLAKQNRAFIVEVNPMPTM
                     LSSALVDIQLAESAGKALTAILSELERRDSGKRTEYNGE"
     gene            complement(7063..8157)
                     /locus_tag="PLM79_14880"
     CDS             complement(7063..8157)
                     /locus_tag="PLM79_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOV87642.1"
                     /translation="MTALKKAYVSVAVMIFLMGAAPAATWSSAQTVAQMGAMQVPFYS
                     IPTRLDFCGEPVPLDMEEVRERFDREFTIVVYSHAQVFLWLKRAQRFFPWIEKQLVHN
                     NLPNDLKYVAVAESDLLVGATSPAKAAGPWQFIPSTGSNYGLSQTGQIDERRDFELAS
                     NSAFRYLRDLQAMFQNWTLAVAAYNCGEKRIQEEIRRQKVNSYYLLKLPMETERYIFR
                     ILAIKEVLSNPAKYGYMLPKGAGYQPVRTDRVALNLKHTTPLQMIAEAASITYRELKV
                     LNPAFISDTLPKGSHTVKVPEGKGRELPQRLDALLATYQPPFVHHKVARGETLSGIAA
                     KYQVSARSICEWNQLKGNKVLAGQTLKIAR"
     gene            complement(8398..8814)
                     /gene="ndk"
                     /locus_tag="PLM79_14885"
     CDS             complement(8398..8814)
                     /gene="ndk"
                     /locus_tag="PLM79_14885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOV87643.1"
                     /translation="MERTLSIIKPDGVARNLVGEIIKRFEEAGIGIVAMKMVHLSREE
                     CRAFYHVHRNRPFFDSLTEYMSSGPIVAMVLDGEDVIRRNRELMGATNPKDAAPGTIR
                     HAFGSDVEKNVVHGSDSAENAAVEIAFFFNALEICG"
     gene            complement(9147..9566)
                     /locus_tag="PLM79_14890"
     CDS             complement(9147..9566)
                     /locus_tag="PLM79_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOV87644.1"
                     /translation="MARGSIIGVLMIVLIGLLMWTGRVGAQPETIVLEHKDVFKELQR
                     PAVKFNHEVHAGAYPDCVECHHVYETKGGKKVNVWSGEGQPCSECHKIEDSGKIPALR
                     EAFHENCTGCHRKLAQGGKKTGPVMCGECHVWPKAAK"
BASE COUNT         1804 a         2999 c         2821 g         2010 t
ORIGIN      
        1 ggagggcctt cctcgatctg gatctccacc cgaacgtcct ttcccaccag gggcaggacc
       61 ttgtgggaca ccacccgggc gtgatagaag ccgcggctct ggtagtattt caccaagaga
      121 tccaggtcct tgccgagaac ttcttcgtcc aggggggcct ttccgaagaa cttgaatcgc
      181 ttttccttgg tggtgaaaat cttcttgagc tcgcccgtcg aaagggatcg aacccccttg
      241 aattgaatca agcggacctg gggcggggaa ggcgatttct ccttttcctc ccgggcatgg
      301 gcataaaccg cctgcccggc gaggaggacc aggagaagga ccaggcgaag ggggaggcaa
      361 gaaaagggat caactccgag ggcggccttt ccggatgccg gcgccgaact ccggggcgat
      421 ccattccccg gatcgttggg cgatgagggt gatggcagag ttccacacac atccatacgg
      481 tatccgggtc caagacgggg gctcacgccg cgggttgtgg gtccctcgaa tctctaacgc
      541 agttgcttga gcttcttttc ggctgcttga gcctggggag tatccggata cttctccaag
      601 agccgttcgt agagaatcct tgccgccgtc gaatccccca gggcctggaa ggccgccccc
      661 tgtttgagca gagtgtgggg caccttgttt cctttagggt atcggtccag cacctgttgg
      721 aaggttgcaa tggcatcctg gtagcgtttc tcggaatagt aacactcccc gatggtgtac
      781 agggcgttgt cggccagctc ggacttcgga tacttgttga gaaacgcctg aaattctttt
      841 ctcgccccgt cgtaccgacc ctgatgaaag agatcgaggg ccttgtcgta gagccccttt
      901 tccgtcggtt ccttctcggc cttttccgga atggtgatca cgggctgcgc aggggcggag
      961 gacttggggg cgtccggtgg ggccgagggt tcttgagcgg agagggaagg ggccggagta
     1021 gcggacacgg ggggggctga ggaaacggag gcctgggatt tggacatacg gtcgatgcgt
     1081 cgatccaact cctcgatgcg gccgttgagc gccccgacct tcagctggag ttcttccaac
     1141 cgggcataga ggtccgcact ctttccgccc tgaccttccg cactgcgaac tcgattttca
     1201 aggttctgca cgcgttcgtg gagcatgccg atgctctgtt gcatggtgga ggtttcctgg
     1261 gtagacgcgc agccgaacaa cgttccaatc atgatcagaa tgactcccgc tcttaggtct
     1321 cggcgattgg aagcatccat aggctttcct tcctccgcaa tttggatgga gatcggctga
     1381 gggcagcccc tcccgggggg tgcacctccg aagaatcata cccgggcgga acaatgctcc
     1441 ggactcgagg gcccggctgg gacgggatcg aggcccgccc ctatcgaggg accgatccgg
     1501 gcgagacggg tccctgctcg aggccgccgc tgatttcccc cggttcatgg ggtcggggcc
     1561 gtttatcgaa gatgcgcaac ggcccccgcc ccatcgagct agtagttgag aacaaaatgg
     1621 gccctgcggt tcttggccca ggatgcctcg tcatggcccg gagcgatggg acgctcttcc
     1681 ccgtagctca ccgatcccaa ccgctgggca tttaccccgg aattcaccag gtattgatag
     1741 gcactgttgg cccgccgttc tcccagggcc aggttgtact cgttggtgcc gcgctcgtcg
     1801 caatgccctt ctacggtcac cttgaccttg ggataccgtt tgagaaactc gaccttggtg
     1861 tccaggaccc ttttggccgg atcgctcagc acgtacgagt caaagtcgaa gtagacgtct
     1921 tcgttctcaa agctctgtgc ccgttgaaga aactcgcgac gttcgggttc cgagttcagg
     1981 cccaattccc gccaccgggc gtcgtccggt cccatgcccg cacccgtgcc cacccccgca
     2041 cccccggggc ctgctccggg taccgcgccg ggctgaggcc cgactttctt ggcacagcca
     2101 atcgtacccg ccacgagaaa aaaccccacc agaacggccg tcatccctct agttcgcctc
     2161 atatccatcc tcctccatgc agacctgttg agagatccct ttcgaaaagc ccctaaatcc
     2221 aggtgattgt ttaccaatcc ggtacctttc ctcccccgtt gacgagggag gtcgggcgga
     2281 gagcggctct cggaccctcc tcccctcccc caaccctgcg ttcaaggcga ggggccggga
     2341 cgcctccccg ggatacccct aacggccgcc gagtcgcggc gaccagtccg gcagttcctg
     2401 ggaaccgggc attcgagtga cccttcgagt cccggcaccg ttggtcagca gcatccagat
     2461 ggcggagccg ccctgccgag tcgagctaaa agcaatcatg cgtccgtccg gcgaccaggt
     2521 aggcgattcg ttgtcccctt ccccccgcgt gagctgacgg acgtcgctcc cgtccggttt
     2581 gatgatgaag atattgtgcc tcccgcccac ggagccgctg taggcgatcc agtccccctt
     2641 gggagaccag gcgggggagg tattgtaatt gccgctgaag gtgattcttc gcttctggcc
     2701 cgacccgaca tccagcacgt agatctgcgg ggtgccggtt tcgttggaaa cgtaggcgag
     2761 tttccttccg tcgggagacc agctgggaga cacgttgatg ctccatccct gcaccagttt
     2821 ctggaggatg tcccccgacg acgagatgag gtagatgtcg gggttgcctc cccgggaaag
     2881 ggtggccgcc agatctcggc tgccgggcct ccaggccgga gcgatgttca agcccgggtg
     2941 tcccgacaag agcctctggg tcccatccag gagattggcc gcataaatct tggtgttccc
     3001 gtccttgaag ctcacatagg caatctgggt cccatcgggg ctccaggtcg gggagaggtt
     3061 gaggttttgg tcgtgggtga cctgaacggc gttgcctccg tcaaagtccg aaacaaagat
     3121 ctctttggcc ttcccctggg actgaacaaa ggtgatcttg gtgtcgaaca ctcctctttc
     3181 gccggtaagg agcagcagga tttcatcggc aaaccggtgg atcatggcgc gccagttgcg
     3241 ggcatcccct tcgtagacct ttcccaggat catcttggac ccgaacacgt cgaagaggcg
     3301 agcctcgagg cgaatggagt tcccctggac ctggtaggcc cctcggacca ggaattcggc
     3361 ctgcaggctc tgccaggtgt tgaaacgaat ctccgcggca ttgagcccca tggtctgggg
     3421 atcctcgagg aaccccttgg gatccagggg ccgaaaaatg ccggaaaact cgaggtctcc
     3481 cgccagggct ccgctcatct ccttggacag ctgcacctgt tccgagctca aaaacttgaa
     3541 atcggggacg gcaatggaga tgggggcgaa actgggctgg gtgatgtcga tgtaaacccg
     3601 ctgggcccgg ccggaatcgg tccacagcag taccattaaa acggccgtga gcaaacgaac
     3661 atacttcatc acactgagac ccctgttctg ggtggaatcc attttggtaa aggccgggtg
     3721 cgacctatgc cagatcctca ggccgaaaac gcaggccgat ctcctcttgg ggagggctgt
     3781 agatctccgg aaaggcgggc aaagggtccg ccttacgcac ggcgcgcatc acggattcat
     3841 cgaagagcgg ctggccggat tgcttctcga aacggatgtt catgaccttt ccgtccctgc
     3901 gaaccaccag cacaagaacg gcctccagtt gctgggactt gagggactcg ggcagggtcc
     3961 actgtcggcg gatggcattc cagatttcgg tatagtaaag gcgacgggcc agggccacct
     4021 gccctccttc gcgattggcg ccgactcctc ccgagccgct gcccttggaa gacccggccg
     4081 gagaggtcga agtgcccttc tccctgctcc gatccacccc tcgagcccca tcggccccgg
     4141 ccgattgctc gctttgggca ggtttcctgt cggcctgatc ggccgaggaa gccggagagg
     4201 aggtcttggc tttggggatc agcttgtcca ggtctttgtc gatggcggcc gaggcctggc
     4261 ggttttcggg ggcctttgcg atttgggggg cctccagttt cttgatggcg tggtcccggg
     4321 gagccgactc ctccatccgc aaccgtttga ccggcaccac gggagaggac ttgccccgct
     4381 gggaggaagc cgcgctcttg gaggccgaag attctttcgg gccttcggat tttagggatt
     4441 tgcttcccgg gggagccacg gccccgcccc cacccagttg gtccatggag acgagattta
     4501 cggtggtgaa ctgcagcggg gttgtggatc ggggcatcat cgacggcaaa aggagcgccg
     4561 ccccgaaaat gagcacatgc cccaagaccg aaccgcccat tcctgcgagg acctcttccc
     4621 tcgacagtct gcgaggtgaa agactcagtg cttccatcct tcaatcctcc acacccatgc
     4681 ccggcaacaa tccccattcc tgctctattt gccccgggat ccggggtcct tggcgggcgg
     4741 gcttttcagg ggttccgtaa ccattccgat ctgatcgatc ccggcctggc gtatcgttcc
     4801 cattacttgc accacaaacc cgtaggggat ggactcgtcg gccctcagga aaacgccttt
     4861 cctgcgctcc aggttctgat acagggcggc cagcttcgcc ccgagggcat ccaggtccac
     4921 ggagtattcg ttgatgtaga cctgctggtc cttggtgatg gtcaccatga gccgctcttc
     4981 ctccgtagga atcgccgggg cggaggattc gggcaggttc acatcgatgc cctgggtcat
     5041 catgggggcc gtcaccatga aaatgatgag gagaaccaac attacgtcta caaaaggcgt
     5101 cacgttgatt tccgagacca actggccccg ggagccgttc ccgttcgttt gcatcgccat
     5161 ggttgccgac ctttgtcaaa tcacccgggg gatcagcccg gggcgttgcc gtaaagggga
     5221 ttcaaaaacc cggcctgcgg cattgcttgc cctcggtccg gaaaggagga ccgaggcccg
     5281 atgtttcccg tcccgccaaa ccctcacgcg gggccggatt tcggtcggtc gcggagaccc
     5341 gccgggcccg gacctcattc ctggacggcc aaccgctggg gttccgccga agactcctcc
     5401 tgctttccct tgcgcatgag gtcgcgcttc aggatgttga gaaaatccgc ggaaaaatgg
     5461 ctcatctcgt gatcgatcat ccggattcgt gtcgcgaaat gattgtagcc caccacggcg
     5521 ggaatggcgg ccgccaggcc catggcggtg gcgatgaggg cctcggagat ccccggggcc
     5581 accaccgcca ggttcgcggc gcccttgagc ccaatctcct ggaaactggt catgatgccc
     5641 cacacggtgc cgaagagccc gatgaaggga gccgtgctcc cggtggtggc caggaggggc
     5701 aggtagcgct cgaaccgctg gcgctcggcc atggtggccc cctggatcgc ccggtccacg
     5761 ctctcgagca ccacctcggg attgaccttc aattcctgga ggttcttggt ctccagggac
     5821 ttgctcagtc gattcagctc gagatagccc gtctggaaga tttgcgccag gtgactgtct
     5881 tccagaagct ggctgtcctt gtaaagaagg gaataattct ttctctgtcg gaagtaatcc
     5941 acgaaccggt ccgtttcctt ccgagccctc cgaaacagtc ggtacttgag caaaatgaca
     6001 caccagcagg caacggacag gcccaacagg gacagcatca cgaacttgac catgggacct
     6061 gcgtggacga ccatgtccca gaccccgttg cccgccctct ggtagggctg tgcaaccacc
     6121 tccgccgccg cataggcggt taaaatcagg ctattcacca ttgtactccg ttcgcttccc
     6181 ggaatcccgg cgctccagct cggagaggat ggccgtgagg gccttccccg ccgattccgc
     6241 caactgtatg tccaccaggg cgctcgaaag catcgtaggc atggggttca cttccacgat
     6301 aaaggccctg ttctgctttg ccaggtgggg caggtgagag gcgggagcca ccgtcgccga
     6361 agtccctaca atgagcatca tgtcgcagga tcgagcggca agacgggctt cctccaagac
     6421 cgacggcgga atcatctctc cgaaaaaaac aaaattcggt cgcagcgagc ctccgcacgg
     6481 acacaccggg ggaaccgtct ccagggtcag agcccctcga tcgaagctcc ggccgcaacc
     6541 gtcgcagcgc agctccctgc cgttcccgtg gaactcgatc acccgcacgc ttcccgcccg
     6601 ctgatggagg ctgtccacgt tctgggtgat gatttccttc aggtagccgc tgctttccat
     6661 gcgggccagg gcataatgag cggaattggg attggcccga gacaacagaa ggtccatttc
     6721 caaaagcatc ctccagacct tggccgggtt catccgaaaa gaatcgatgt gcccgtattc
     6781 cagagggtcg tagcgagacc aaagcccatc ctcgctccga aagtccggaa tcccgctctc
     6841 aacggaaatc ccggcccccg tcagggcaat cacgtagcgg ctcgaggcga tgcgttcggc
     6901 gatttgtgga tagcgagggt ccattgtcca aatttcattg aatttctaat gccttatgtc
     6961 ggcggtatcc taagtccctc ccggggcctt gtcaaggcat tttgccaaga gattccgttt
     7021 gctgcggccg atccgaccaa gggcagggtt ttcctcccga cactacctgg caatcttgag
     7081 cgtttgtccc gcaagtacct tgtttccctt gagttgattc cattcacaaa tgctccgggc
     7141 actgacctgg tacttggcgg caatgccgct caaggtctcc ccccgggcca ccttgtggtg
     7201 cacaaaggga ggttgatagg ttgccagcag ggcgtccaga cgctggggca attccctccc
     7261 cttcccttcg ggaactttca ccgtgtggga acctttggga agggtgtccg agatgaaagc
     7321 aggattcagg accttgagct cccggtaagt aatggaagcg gcttcggcga tcatctgaag
     7381 gggggtggtg tgtttgagat tcaaggccac ccggtcggtt cgcaccggct ggtaacccgc
     7441 ccccttgggg agcatgtacc cgtacttggc ggggttgctc agcacctcct tgatggccaa
     7501 aattcgaaag atgtagcgtt cggtttccat cgggagcttg agcaggtaat agctgttcac
     7561 cttctggcgc ctgatttcct cctggattcg tttctccccg caattgtaag cggccacggc
     7621 cagggtccag ttttgaaaca tggcctgcag gtcccgaagg tagcgaaagg cactgttgga
     7681 cgccagctcg aagtcccgcc gttcgtcaat ctgtcccgtc tgactcaggc cgtagttgct
     7741 gcccgtgctg gggatgaatt gccacggacc tgcggccttg gccggggagg tggctcccac
     7801 gagcaggtcg ctttcggcaa ccgccacgta cttgagatcg ttgggcaagt tgttgtggac
     7861 gagctgcttt tcaatccaag ggaagaaccg ctgggcgcgc ttgagccaca gaaagacctg
     7921 ggcgtgactg tacaccacga tggtgaactc ccggtcaaaa cgctcccgga cttcttccat
     7981 atccaggggc acaggctccc cgcaaaaatc caaccgggtc gggatcgaat agaagggaac
     8041 ctgcattgcc cccatctggg ccacggtttg agcggagctc caggtagctg cgggagcggc
     8101 ccccatcaga aaaatcatca ctgcaacgga cacgtaggct tttttcagcg cagtcatgca
     8161 cttccccttc ccctgacttt cgtgaaccag ggacccggct taccccacgg gcaccgcatc
     8221 cgggtacctt cgaacaaaga cccgccccat ccctctgccc gttcctccac tacggggacg
     8281 acgccgggga agtcttgatc atcccgggaa tcccccgaaa aatcaaggga gaacgcccgg
     8341 ggacccggtc ccccccatgt cctaacccga tgatgacccg gatacccgtc cgtacttcta
     8401 cccgcagatt tccagggcat tgaaaaagaa agcgatctcc acggcggcat tctccgcgct
     8461 gtcggaccca tggacgacat ttttctccac atcggatcca aaggcgtgac gaatggttcc
     8521 cggggccgca tccttgggat tggtcgcccc catcagttcc cggtttctcc ggatgacatc
     8581 ttccccatca agcaccatgg ccacgatggg ccccgaagac atatattccg tcagactgtc
     8641 gaagaaaggc ctgttccggt gcacgtgata gaaggcccgg cactcctccc ggctcaaatg
     8701 gaccattttc atggccacga tgccgatccc ggcctcctcg aatcgtttga tgatttctcc
     8761 aaccaggttt cgggccactc catccggttt gatgatggac agggttcgtt ccattcggct
     8821 tcctccgttc caggatatcc cggggggcga ctccccttcg ggtccctact ctgatgcctc
     8881 tagtccgagg aaaagtcaaa cggcttttca ttccaggggc aaaagtgcct gccccggggg
     8941 gtcttgcccc cgggacccga ctcgagagca aaaaaaagac gacgccggtt tcttttgctt
     9001 cttccggcgc cgtctttcat atgttacggg gttggggggc atgcccccga atccgggatc
     9061 gttacctgcc ggtgctccgg agcgattggc aaaggtcccg aagtctccgc cgtcggcccc
     9121 gggaccccaa tcccataagc ttcacctcat ttggcggcct tgggccatac atgacactcg
     9181 ccgcacatta cgggtcccgt tttctttccc ccctgggcaa gctttcggtg gcatcccgtg
     9241 cagttttcgt gaaacgcttc ccttaaggca gggatcttgc cggaatcctc gatcttgtga
     9301 cattcggaac agggctgccc ttcgccgctc cacacgttga cctttttccc gcccttggtc
     9361 tcgtagacgt ggtggcattc cacgcaatcc gggtaggcgc cggcgtggac ctcgtgattg
     9421 aacttgaccg ccggtcgctg cagttccttg aagacgtcct tatgttcgag cacgatggtt
     9481 tccggctgcg cccccacccg accggtccac atcagcaatc caatcagcac gatcatcaaa
     9541 acgccgatga ttgaccccct cgccatgcgt ccttctcctt ttcttgggta ccgaaccacc
     9601 attgctcccc tcccccggga cctactcttc cccc
//
LOCUS       DAOOVC010000148         9535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4525_length_9535_cov_5.068776, whole genome shotgun sequence.
ACCESSION   DAOOVC010000148 DAOOVC010000000
VERSION     DAOOVC010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9535
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4525_length_9535_cov_5.068776"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..695)
                     /locus_tag="PLM79_14895"
     CDS             complement(192..695)
                     /locus_tag="PLM79_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV87645.1"
                     /translation="MLFCFNAAEVFQIAVEIEENGKAFYDKARELVTDPEVKKLFADL
                     ALEEVSHKKKFEDFKAQLPSGLAAPTVSDPQHELDLYIKMMADQHVFRTTKELDAQVS
                     KIQSAADALKLAIQFEKDSVLFFLSMQEATCEGKGRDLVTLLVKEEQEHVRRLSLQLR
                     KITHSAG"
     gene            complement(726..884)
                     /locus_tag="PLM79_14900"
     CDS             complement(726..884)
                     /locus_tag="PLM79_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOV87646.1"
                     /translation="MDRYVCSICGYVYDPALGDPENGIAPGTPFEDLPDDWVCPPCGA
                     PKSEFEKQ"
     gene            complement(1011..2102)
                     /locus_tag="PLM79_14905"
     CDS             complement(1011..2102)
                     /locus_tag="PLM79_14905"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV87647.1"
                     /translation="MALRLSFRFRVVVVSWALFVLALFPALNHYFAECDRALASRARD
                     DARRQLEFMDYLLRQGHAPGGALGLQEWVRTMGKQSRLRITYVGRGGRVIADSELSSE
                     EVADLDRHVSRPEIMGAGELEIGISTRYSATMGTELLYAAKKVEGFDDLPPGVLRIAV
                     PLLPVPGVFGRFGIPLLWILAGSLAALSLVAHFATRRYRRVLEDLSRASAALASGDLH
                     QRFAADSREEALPAVQSMERLARNLQGMLRESSRKDRILEAVTEEMSEAFLLLDADGR
                     VRRANQRFCDLFPGSVPAEGHRPLELVRSPELQEAVDEVLRGKDLPGDPGSRRPAFHW
                     RGRSHQAVVIPIREEGETTGAVALFTAAG"
     gene            complement(2507..2902)
                     /locus_tag="PLM79_14910"
     CDS             complement(2507..2902)
                     /locus_tag="PLM79_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV87648.1"
                     /translation="MAVAAGYKNVNWMRVGIKGWHKAFYAVDSDSKLFMTLMDLNQGN
                     PSSWVVDLPEAGTLKNPTFLDIRPAARFEAGHLDRAINIPYAEMWTFENFEKLNKSKD
                     IVLIHDDPVVSGAFAMTLRLLDYRAFILQ"
     gene            complement(2927..3217)
                     /locus_tag="PLM79_14915"
     CDS             complement(2927..3217)
                     /locus_tag="PLM79_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87649.1"
                     /translation="MSEAPKGQTPTSLEGVRVVSPKEARKIQGEGGLLIACYTHMTDW
                     VQARPVGSVHITMDVPKDHKRTDFDLKDAEFDLSALPADKDRPIVLFCADDM"
     gene            3792..4151
                     /locus_tag="PLM79_14920"
     CDS             3792..4151
                     /locus_tag="PLM79_14920"
                     /inference="COORDINATES: protein motif:HMM:NF028155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004869 - cysteine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin domain-containing protein"
                     /protein_id="HOV87650.1"
                     /translation="MKLKSLLLIVLVSVAVSSTGSCQDMAEPRIPRAGGWFPASVDEP
                     EVRAAADRAVAVRSATAGEELKLIAVREARSQVVAGRNFELVLAVERNGKRVSAKAVV
                     WAKLDGTYQLTQWSWEK"
     gene            complement(4285..5949)
                     /locus_tag="PLM79_14925"
     CDS             complement(4285..5949)
                     /locus_tag="PLM79_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOV87651.1"
                     /translation="MKEHHTVSRYLLGRLHEIGIQHLFGVPGDYVLDFLDEVIAGPIQ
                     WVGNCNELNAGYAADGYARLNGAGAAVVTYGVGGLSILNAVAGAYAEHVPLVVISGAP
                     PSRRRESGAMVHHLVSDYFLQLDIYRKLTADAVILTDPSTAPEEIDRVLGHCIARKLP
                     VYLEIPADIAVAPCRRPGKLHEIPLPPSDPAVLAECVSEAAEKINRADHPLVLAGVEL
                     VRFGLGGDALRLVETVEIPFSTMVSSKSALPELHPQFVGLYQGGWSREAVRRQVEESD
                     CLLSLGVWMTDLDTGMFSMNLDDRRMIAAGGGQVRIGHHYYPQIRLKDFIERLTPAVR
                     PRSYLASHPAESYRPKPPFAPEKSKMLTAPRLYQCIEHFLDDRMILLAEPGDAFCAAP
                     EFHIEEAENFLVQSYYASIGYCTPAALGVALACPGKRPVVLTGDGAFQMTAQEVSTLI
                     RHRCPALILVINNDGYLIERLLHEDGPYNDLQRWDYAGLPRVFDSGSRSVGMRVSTED
                     ELDGAMKIAAGEKDRLVLIEACLPNRDCSAGLRRLGDTFRKARQKR"
     gene            complement(6032..7240)
                     /locus_tag="PLM79_14930"
     CDS             complement(6032..7240)
                     /locus_tag="PLM79_14930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOV87652.1"
                     /translation="MKRTSRYWYPGLILLSVFWFSGNADGRTSLDSVLQPYLSSYDLP
                     AVAAGVVRDGKVIAAGAVGTRRVGTHIPVTLQDRFHIGSDTKAMTALMAAMLVEEGKL
                     RWDTAVAEVFPELAEEMDPRLRRVTLEQLLSHTSGIPKDNEEIIKSYLEAMTQDGNLD
                     AARYWLVRQMSRRPLEFEPGTGFAYSNTGYVFVGAMIERLEKTTWEEAITRRVFIPLQ
                     LKTAGLGPQASMGKIDAPVGHAVVDGRPQALLAGPNGDVPPVMGPAGTVHLSVLDFAR
                     WAGWNAGEGKCKPALVKPETLRKLHTPVISTPEKKDAAPGTPRGGKYALGWGWMTVDW
                     APYPLLYHGGSNSMNLAHVWIDPQRDLAMVFVTNIGGPKADAALLHLAHKLYSKYGRK
                     GIGPLSTTGR"
     gene            complement(7316..8206)
                     /locus_tag="PLM79_14935"
     CDS             complement(7316..8206)
                     /locus_tag="PLM79_14935"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HOV87653.1"
                     /translation="MIRHRRRFLIALAILCLPALEPVRAETQGHVSPGGTEVALPLPL
                     SSGSTVRPARFHLARNTDRSASRTNATYSSESHTRRLWDENYPFYLKILNMPFNRELL
                     SGRLDEKIFRNYIIQDYHYLQNYRKVYGILLAKAPDETAARFIVTLIHGIDEEIARIH
                     HTYMKKLHISGEELSSTPPYPSTEFYNSFLIKTAALEPFEVGLAATLPCHWIYYQLGV
                     DMKKSQPGKESKYREWIDGYGAGPWEDSETKKVVDFVEDWMRKTTDETRSRMKKAYET
                     AMKLEYLFWDGVYRDVKWVE"
     gene            complement(8457..8873)
                     /locus_tag="PLM79_14940"
     CDS             complement(8457..8873)
                     /locus_tag="PLM79_14940"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOV87654.1"
                     /translation="MSHPAAKPDRLGITGFRRGASYTILYAARSRAVNAAVAWYGQIK
                     MPHTPGVRTVSPLEVSGKITASFLGLYGDADAVIPAADVRGMEAVMKAVNRTAEFVLY
                     PGAPHALYADYRPTSYHADAAKDAWNRCLEWFKKYL"
     gene            9286..>9535
                     /locus_tag="PLM79_14945"
     CDS             9286..>9535
                     /locus_tag="PLM79_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87655.1"
                     /translation="MSDRTKLCGRYERKCNALFLLSLLIFLHPGTAISKTPSYGIILI
                     GQSNAEGQGENSSLNESMLNVPDSVEFYDKGERILRYDQ"
BASE COUNT         1788 a         2857 c         3029 g         1861 t
ORIGIN      
        1 ccccgggaat cggctccact ctccaattct gtttgcgggg gcagtagggg ggctgatgga
       61 ataatgagta ctatgcccga ggaaaaagca gaagaaaaat ccgtcgctgc cgaaaagttt
      121 tcccgggagc gacggatgcg cctttcgagg aagcggaatc cggatttcaa cttctccgag
      181 accgaagagg cttacccggc cgaatgagtg atcttgcgaa gctgcaagga aagccttcgg
      241 acgtgttcct gttcttcctt caccaacagg gtcaccaggt cgcgaccctt gccttcgcag
      301 gtggcctcct gcatgctcag gaagaacaga accgagtcct tttcaaactg gatggccagt
      361 ttcagggcgt ccgcggccga ctgaatcttc gagacctggg cgtccagttc tttggtggtg
      421 cgaaacacgt gctggtcggc catcatcttg atatagaggt ccagctcgtg ttgcgggtcg
      481 ctcaccgtcg gcgccgcaag cccggacggg agctgcgcct tgaagtcctc gaacttcttc
      541 ttgtgggaca cttcttccag ggccaggtcc gcaaagagtt tcttcacttc ggggtcggta
      601 accagctccc gggccttgtc gtaaaacgct ttgccgttct cctcgatttc cacggcaatc
      661 tggaacactt ccgcagcatt gaaacaaaaa agcatgagca tctcctcctt ggggcagggc
      721 cggttctact gcttctcgaa ctcgctcttg ggagctccac agggcgggca gacccagtcg
      781 tcggggaggt cctcgaacgg ggttccgggt gcaatcccgt tttccgggtc gcccagggcc
      841 gggtcgtaaa catacccgca aatgctacag acgtaacggt ccatcaaagc ttctcctcct
      901 cttgtcctct gggtagtccg tctatgataa gcgctccgaa tgaatctgcc catatcacgg
      961 ggaatgattt cgtgcaacgg attcgtgagc gtcaggggac ccattcggtt ctaaccggcg
     1021 gccgtgaaaa gggcgacggc cccggtggtt tccccctcct cgcggatggg gatcaccacg
     1081 gcttgatggg atcgccctct ccaatggaaa gccgggcgcc tcgaacccgg gtccccggga
     1141 aggtcctttc cccgcaacac ctcgtccacg gcctcctgga gctccggact ccggaccagt
     1201 tccaaggggc ggtgcccttc ggccgggacg gacccgggaa acaggtcgca aaacctttgg
     1261 ttcgcacgac ggaccctgcc gtcggcgtca aggagcagga acgcttcgga catctcctcg
     1321 gtgacggcct cgaggatccg gtcttttctc gaggattccc ggagcatccc ttgaaggttt
     1381 cgtgccagtc gttccatgga ttggaccgcg ggcaaggctt cctcgcggct gtccgcggca
     1441 aaccgctgat gaaggtcccc ggaagccagg gcggcggacg cccgggaaag gtcttcgagg
     1501 acccggcggt agcggcgagt cgcaaaatgg gcgacgaggg acagggctgc cagggagccc
     1561 gccaggatcc acagaagggg gatgccgaat cggccgaaga ccccggggac ggggaggaga
     1621 ggaacggcaa tgcgcaagac tcccggggga aggtcgtcga acccttccac cttcttggcc
     1681 gcatacagga gttccgtccc catggtggcg ctgtagcgag tactgatgcc gatttcgagt
     1741 tccccggctc ccatgatttc aggccgagag acgtgacggt cgaggtcggc aacttcctcg
     1801 gaagaaagct ccgagtccgc aatgacccga cctccgcgac ctacgtaggt gatgcgcaac
     1861 cgggattgct tgcccatggt gcggacccac tcctggaggc ccaaggcgcc gccgggggcg
     1921 tgcccttgac ggagcaggta gtccatgaac tccaattgcc gccgggcgtc gtcccgggcc
     1981 ctgcttgcca gggcccggtc gcattctgca aaataatggt tcaaggccgg gaagagagcc
     2041 aagacaaaca aggcccagga gaccacaacg actcgaaacc ggaaagaaag cctcaaggcc
     2101 ataccattga ccctccggga cctctcgggt ccccgttgcg ttgagtgtgg aaaagggtat
     2161 ccgccgggac ggtttcccgg gagcgccccc cgttcaagcc ctgccggtga gctcattgaa
     2221 cgggtgcgtt tcgggaaccc gccgggtatt gtaccggttg gaggccgatt gtcgaaacct
     2281 tatcttgggg gtccgaagga gggagacagg gcccgcaggc aaggatcgcc tgcaggcccg
     2341 ggggtgggca atgcggttga cgaaagccgt gttcgtcctt cccgcacagt cgggaatccg
     2401 ggtttttcca gggattcccg gacttcctct ccccgcctgg ttcctgcaag gagaggggga
     2461 gggtaagtca accgcattgc ggggggcaga ggtcgagacc ggacgactac tgcagaatga
     2521 acgcccggta gtccaacagg cgcagagtca tggcgaaggc gccggaaacc accggatcgt
     2581 cgtggatgag gacgatatct ttactcttgt tcagcttttc gaagttctcg aaggtccaca
     2641 tctcggcgta ggggatgttg atggcccggt ccaggtgacc tgcttcaaac cgggcggccg
     2701 ggcggatatc caggaaggtc ggattcttca gggtcccggc ttcgggcaag tccaccaccc
     2761 agctggacgg attcccctgg ttgaggtcca tcaatgtcat gaacagcttg gagtcggaat
     2821 ccaccgcgta aaaggctttg tgccatccct tgattcccac ccgcatccaa ttcacgttct
     2881 tgtagcctgc ggcaactgcc atcctggcag cgtgatagga cttccatcac atgtcgtcgg
     2941 cacagaagag cacgatgggc cggtccttgt ccgcggggag agccgaaagg tcaaattccg
     3001 cgtccttgag atcgaaatcc gtacgcttat gatccttggg tacgtccatg gtgatgtgga
     3061 cggagcccac cggccgggcc tgtacccaat cggtcatgtg ggtgtaacag gcaatcagca
     3121 ggccgccctc tccctggatc tttctggcct ctttgggcga taccaccctc acaccctcta
     3181 aactggtggg ggtctgccct ttgggtgcct cactcatgtt gctctccttc cgctggtgga
     3241 tgattgaagg tttctccccc cgtcgctgta ttcccaggac gggtcggaaa acctctatca
     3301 aaaccgatgc caatgagttt ccctgcggtt ttacaggaaa atggatgcgg gaaggacccc
     3361 ccgcatcctg tacccggaga cgcatctgtt ccgtgacatg tcccggggac caggcactct
     3421 gccgggcgac ggctccgtgc cggcgggggg cggtcttctc cctgaatcta ttcccaaaac
     3481 ccgggacgaa tggggtcccc gaccgggcag gcttcgcgca ttcctgcagg gttggggagg
     3541 ttcaaatttt gccaaggatg ccgaaacaaa tggtatcctg cggacgtggg accccccggc
     3601 gtcttgtcga ggcaggccgg aacccgaaaa gaagggaccg aggacggcgt tcgccgggga
     3661 ggagacggac ggtcgtcggt tgttcgaaag acgacactcg attttgtcgg gaagagcagg
     3721 cggatcgcgc cataggaaag gcgatatgca agccgtctac tccttggggc gcggtcccgg
     3781 aggaggatcg gatgaagctg aaatcactgc tgctgattgt cctggtatcc gtggctgtga
     3841 gctcgacggg gtcctgccag gatatggcgg agccgcgcat ccccagggcg gggggctggt
     3901 tcccggcttc ggtggacgag cccgaagtcc gggccgcggc cgaccgggcc gttgcggtgc
     3961 gatccgctac agcgggagaa gagctgaagc tgatcgcggt acgggaggcc cgatcgcagg
     4021 tggtggccgg gcggaacttc gagcttgtcc tggccgtcga gcggaacggg aagcgggtgt
     4081 cggcaaaggc cgtcgtctgg gcgaaactgg acgggaccta ccagttgacc cagtggtcct
     4141 gggaaaagta gaccccgcat cccgggcacc tctttccatt tccctgcggc tgggggttcg
     4201 ggatcgcgct gccgggtttg gccgtcggcc cgtccatcct tatgtccctg tgaaagacgg
     4261 ccccccgagg ggcgaggcgg ggacctaccg tttctgccgg gcctttcgaa acgtatctcc
     4321 caggcgccgg aggcctgcag aacagtctcg gttcggcagg caggcttcga tgagcaccag
     4381 ccggtccttc tccccggcgg cgatcttcat ggccccatcc agttcgtcct cggtggaaac
     4441 ccgcatgccg acggaacggg atcccgagtc gaacacccgg ggcagtcccg cgtaatccca
     4501 cctctggaga tcgttgtacg ggccgtcttc atggaggagc cgctcgatga ggtagccgtc
     4561 gttgttgatg accaggatca gggcgggaca gcggtgccgg atgagggtcg agacctcttg
     4621 ggccgtcatc tggaaggccc cgtcccccgt gagcaccacg ggacgctttc cgggacacgc
     4681 cagggccacc cccagggccg ccggggtgca atagccgatg gaggcgtagt agctctggac
     4741 caggaagttc tccgcctcct cgatgtgaaa ttccggcgcg gcacagaagg cgtctcccgg
     4801 ttccgccagc aggatcatcc ggtcgtccag gaagtgttcg atgcattgat aaaggcgggg
     4861 tgcggtcaac atcttcgatt tttcgggcgc gaacggcggc ttgggccgat aggattccgc
     4921 cgggtgggac gccaggtaag aacggggtcg gaccgccggg gtcagccttt cgatgaaatc
     4981 cttgaggcgg atttgagggt aataatggtg gccgatgcga acctggccgc ctcccgcagc
     5041 gatcatgcgc cggtcgtcca ggttcatgct gaacatgccc gtatccaggt ccgtcatcca
     5101 gacccccagg gacaggagac agtcggattc ttccacctgc ctgcgcaccg cctcccggct
     5161 ccaccccccc tggtagaggc ccacaaactg ggggtgaagt tccggcaggg cggacttgct
     5221 gctgaccatg gtcgagaacg ggatctccac cgtttccacc aggcggaggg cgtcgccccc
     5281 cagaccgaag cgaaccagtt ccacccccgc cagcaccagg ggatggtccg cccggtttat
     5341 cttctcggcc gcctcggaaa cgcactccgc cagcacggcc gggtccgaag gcggaagagg
     5401 gatctcgtgg agttttcccg ggcgccgaca cggggcgacg gcgatgtccg ccgggatctc
     5461 gagatagacg gggagcttcc gggcgatgca atggcccagg acacggtcaa tctcctcggg
     5521 ggcggtgctc gggtccgtga gaatgaccgc atccgccgtg agcttgcggt agatgtccag
     5581 ctgcaaaaaa tagtcggaca ctaggtgatg caccatggcg cccgactccc tgcgccggga
     5641 cgggggagct ccgctgatca cgaccagggg cacatgctcc gcataggccc ccgcaacggc
     5701 gttcagaata ctgagccctc cgaccccgta ggtcacgacg gcggcaccgg ctccgttcag
     5761 gcgggcatag ccgtccgccg cgtacccggc gttgagttcg ttgcagtttc ccacccactg
     5821 gatgggaccg gcaatgactt cgtccaggaa gtccaggacg taatccccgg gaaccccaaa
     5881 gaggtgttgg atcccgattt catgcagacg accgaggagg taccgagaaa ccgtgtggtg
     5941 ttctttcatc ccaatctcct ttacggacgg ttgccccgag aggtgccgag gccgtgagac
     6001 ccgaggccgc cgccgacccc gcacaaaacc cctacctgcc cgtggtggag agcgggccga
     6061 ttcctttcct accgtatttc gagtacagct tgtgggcaag gtggagcaag gccgcgtccg
     6121 ccttgggacc gccgatgttc gtgacgaaga ccatggccag gtcccgttgg gggtcgatcc
     6181 agacgtgagc caggttcatg gaattggatc ccccgtgata gagaagggga tatggagccc
     6241 agtcgacggt catccagccc cacccgaggg cgtatttgcc gccccggggg gttccggggg
     6301 ccgcatcctt cttctccggc gtcgaaatga ccggggtgtg gagtttcctc aaggtttcgg
     6361 gctttaccag ggccggtttg cactttcctt ccccggcgtt ccatcccgcc caccgggcga
     6421 aatccaaaac ggacaggtgc accgtcccgg cagggcccat gaccgggggg acgtccccgt
     6481 tgggaccggc caagagggcc tggggccttc cgtcgaccac ggcatgcccg acaggggcat
     6541 cgatcttgcc catggaagcc tggggtccca aaccggcggt cttcaattgc agggggatga
     6601 agacccgccg ggttatcgct tcctcccagg tcgtcttttc aagtcgctcg atcatggcgc
     6661 ctacgaagac gtatcccgtg ttcgagtaag caaagccggt gccgggttcg aattccaggg
     6721 gccgccgact catctgccgg accagccaat agcgggcggc atcgaggttg ccgtcctggg
     6781 tcatcgcttc caggtaggat ttgatgatct cctcgttatc cttgggaatc ccgctggtat
     6841 gggagagcag ctgctcgagg gtgacccgcc gcaacctcgg gtccatttcc tcggccagct
     6901 cggggaagac ctcggcaacg gcggtgtccc atcggagctt tccctcttcc accagcatgg
     6961 ccgccataag agcggtcatg gccttggtgt cggagccgat gtggaagcgg tcttggaggg
     7021 tgaccgggat gtgggtcccc accctgcgcg tgcccacggc accggcggca atcacttttc
     7081 cgtcccggac cacccctgcg gctaccgcgg gcagatcgta tgagctcagg tagggttgaa
     7141 gcacggaatc cagggaggtt cgaccgtcgg cgttcccgga gaaccagaaa acgctcaaca
     7201 ggatcaaccc ggggtaccag taacgggagg tgcgtttcat gagagatcgc ctcctttgca
     7261 atgtcgcttt attgggtccg ggaccgaaaa gggccgcggt gagtcctgcc cgccctcact
     7321 ccacccactt cacgtccctg tatacaccgt cccaaaacag gtattccagc ttcatggcgg
     7381 tctcgtacgc cttcttcatc ctgctccggg tttcgtcggt ggtcttccgc atccagtcct
     7441 cgacgaaatc gaccaccttc ttggtctcgc tgtcctccca cgggcctgca ccgtatccgt
     7501 cgatccattc ccggtacttg ctctccttcc ccggttggga ttttttcatg tcgacgccca
     7561 gctggtagta gatccagtgg cagggcaacg tggccgccaa acccacctcg aagggttcga
     7621 gggctgcggt cttgatcagg aaggagttgt aaaactccgt gctgggataa gggggcgtgc
     7681 tcgacagctc ttccccgctt atgtggagct tcttcatgta agtgtggtgg atccgggcga
     7741 tttcctcgtc gattccgtgg atcaaggtca caatgaacct ggccgccgtc tcgtccgggg
     7801 ccttagccag gaggatcccg taaacctttc ggtaattttg gaggtaatgg tagtcctgga
     7861 tgatgtagtt ccggaagatc ttctcgtcga gccggccgct gagcagttcc cggttgaagg
     7921 gcatgttcag gatcttcagg tagaagggat agttctcgtc ccaaagccgc cgggtatgac
     7981 tctccgaaga gtacgtcgcg ttggtgcggg aagccgaccg gtccgtgttt cgggccaggt
     8041 ggaaacgggc gggccggacc gtggaaccgg atgaaagggg aagcgggagc gcgacctccg
     8101 ttccgcccgg ggaaacgtga ccctgggttt cggcccggac gggctcgagg gcgggcaggc
     8161 aaaggatcgc cagggcgatg agaaaccggc gtcggtgccg gatcatggga ttcctccttt
     8221 ccagggccta atcggaaggg tgaacggcga attgaattgg gagcgtcctc cgggatttgc
     8281 cggttcaggg tgcgagtata acagggtttc ccgtcacttc gccagagggg aacgggattg
     8341 aaaatctgcg aatttacccc gtgcccggga tccatgttcg tggttcccgg aggcacgggg
     8401 attgagtgaa acgaggggct gccggatctc tcagacagag aagcggtgtg tcccgtctag
     8461 aggtactttt tgaaccactc gaggcaccga ttccaggcat ctttggccgc atcggcatgg
     8521 tagctcgtcg ggcggtagtc ggcgtaaagg gcgtggggcg ccccgggata aaggacgaat
     8581 tccgccgtcc ggttcaccgc tttcatgacg gcttccattc ccctcacgtc cgccgccggg
     8641 atgactgcat ccgcatcccc gtaaagcccc aggaaagagg cggtaatctt gcctgagacc
     8701 tcgagggggc tcaccgttct caccccgggt gtgtggggca tcttgatttg tccgtaccag
     8761 gcgaccgccg cgttcacggc gcggcttctt gcggcgtaaa ggatggtgta ggaggcgccc
     8821 cggcggaagc ccgtgatccc cagccggtcg ggtttggctg ccgggtggga caattcccgt
     8881 tgatgttttc ttcattcaag gacttgacat tcccggtata cactttcgca ttcgcgatga
     8941 cttgctcctg ggtgatacgg cacccggtgc cggcctgtac cgaggacact ccttccctgg
     9001 gcgaatcgta agagtaggct tctccagtgc cggagagaag accgggcgcc ggatttccac
     9061 cctttgcgcg tgccgtcaag gcgaatgttt ttctcatccg gcggcgagtt ttcatgaaag
     9121 acgccccgcc gccgcaccgg gaagggcgtt ccggaacggg atccggtgga gccaaaagag
     9181 caggcactgt agcggatttg ttgacgaacg tttaggggag aggccctgca agctgcgaag
     9241 atctcgggaa gttgtggtgg gcgggagagg attcgaacct ccgacatgtc agacaggaca
     9301 aagctttgcg ggcgttacga acgaaaatgt aacgcacttt ttctgctatc gctcctcatc
     9361 ttcctccatc caggcaccgc catcagcaaa actccatcct acggaatcat cctcatcggc
     9421 caatcaaacg ccgaaggcca aggagaaaac tcctccctca acgaatccat gctcaatgtt
     9481 cccgattccg tcgagttcta cgacaaagga gaacgaatcc tccgatacga tcaac
//
LOCUS       DAOOVC010000149         9444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4570_length_9444_cov_6.517627, whole genome shotgun sequence.
ACCESSION   DAOOVC010000149 DAOOVC010000000
VERSION     DAOOVC010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9444
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_9444_cov_6.517627"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PLM79_14950"
     CDS             complement(<1..1732)
                     /locus_tag="PLM79_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOV87656.1"
                     /translation="MRRATCVSSLAKSFFAPSWNSITQAKVPLHLFQGVGSGTAPSEV
                     FEPLFGEANVLEVIQVFENRLAGVKRLSGENVRMKFVPAQVMYLLQDPRAKRNIRSLL
                     RFMAILVFFIGLYSILFHFIMEFEGRDFSWITGLYWTLTVMSTLGFGDITFSGDLGRV
                     FSILVLLSGIVFLLIMLPFTFIQFFYAPWLEAQSKSRAPRELPEGEAGHVILIGFDPI
                     AMSLISRLKQYGHPHAVLVTDVNLALDLYDRGYRVVVGEPDDPETYRRLRADRAAMIV
                     ALEDDMKNTNIVFTVREISKHVPVVANAEEEDSVDVLELAGATHVFQFTKILGEFLAR
                     RTLAGRTSSNMIGHFGELLIVEAPAAHSPLVGRTLLNCGLREATGMIVVGWWEQGVFQ
                     IPKLSDTIQSNTVLVLAGSQDQLARYDDFVGKRSVPDAPALILGGGRVGRAAAQVLAN
                     KGLDYRIVEKNPKVAERCSNIIPGSAADLETLVKAGIRQTPSIFITTHNDDVNIYLTI
                     YCRRLRPDVQILSRATLDRNFNILHTAGADLVMSHASLAAHTIFNLLRPGKVLMLTEG
                     LNIFQVRAHKS"
     gene            complement(2356..2433)
                     /locus_tag="PLM79_14955"
     tRNA            complement(2356..2433)
                     /locus_tag="PLM79_14955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2396..2398),aa:Pro,seq:tgg)
     gene            complement(2852..4027)
                     /locus_tag="PLM79_14960"
     CDS             complement(2852..4027)
                     /locus_tag="PLM79_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOV87657.1"
                     /translation="MEPVDRRLDHPDPLPGDAGEGEARPGEAESGLEAENAELRKRLN
                     GVLEAAAENERIWRHFTEIERILFRTRDLNQLVEELLLEIKDRFEPDQVTLLLCHQDL
                     IQEYFPALSGDSEPIGDGTWIVPCPAAVGVFVCRDPHKPALLSGEELRKLGEYLPDSP
                     EPVQSGVLIPLCVHEVHFGFLFLGSLDAERYRPDDGTDLLEQLGIKIALCMENCLTYE
                     KVKDFSIQDPVTGLLNFFQIHTVLEREFRRARRLEEPLSVLIIDLDFFQDEEGRLDIG
                     GRILTHVANLLKATLPQGEAFLGRFGSDEFLAVLPRVPGEEAREGIPYLAQTIRKSPF
                     KLENAAILIHAHIGVGSMQEHTRRPQELLDAAYAELCQAKLQRRTVGGRPTADRETG"
     gene            complement(4038..4532)
                     /locus_tag="PLM79_14965"
     CDS             complement(4038..4532)
                     /locus_tag="PLM79_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HOV87658.1"
                     /translation="MRRPVLGVIGTGKTAPGLEELALEVGRQIARCGAILVCGGLGGV
                     MEAAARGAKEAGGITLGILPGAGPDDANRYIDIPVATNMGQARNAVIVHTADALIAVD
                     GEYGTLSEMAMALKIGKPVIALEPRFSVPGLVVVKQPKEAVEEAISFVRARCPRLWDA
                     QTGW"
     gene            complement(4697..5341)
                     /locus_tag="PLM79_14970"
     CDS             complement(4697..5341)
                     /locus_tag="PLM79_14970"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOV87659.1"
                     /translation="MINFQKARDRMVETQLIGRGIDDDRVLIAMRKVPRHLFVDEALQ
                     EQAYNDHPLPISDKQTISQPYIVALMTEALHLKGHEKVLEIGTGSGYQAAVLAELAHR
                     VFSMERFASLAHRAGQVLQKLGYRNVIVRVGDGTLGWPEESPFDGIVVTAGAPKVPQT
                     LVDQLAVKGRLVIPVGDRISQELIVVERAPEGIRKHNLGGVRFVDLVGKWGWEE"
     gene            complement(5469..7382)
                     /locus_tag="PLM79_14975"
     CDS             complement(5469..7382)
                     /locus_tag="PLM79_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87660.1"
                     /translation="MKGSSEFPTYDTPGPGAQGPSWCRTLRDHLLLCGHPRKAVREEA
                     LNRVLLFLEDGEPRSYGHLLEAVSSLGPESRLLPPILWRVLCDWMALVHRPGPRTKAR
                     DPVRDFLNKPSGAFFSCLFHRPVPLGLWADFLPPVFFFPGTGRSVSVQRTRWRLLRNR
                     VRRTGSPPWAEPTCRDLLPLWRKKAPRRGRPVPSGRWLRIGRPVLMAPAGNEKIRGSF
                     PGLRQSYWAGCGDRLRSFLDGLIRCQERELALIRDLARTVSLGSRQVVLSWHNATLAA
                     AGGWAFESIEERFPSRCAWEDFKKRVKGRLRDARGRDAARLSRMADLWEERKKRLLAP
                     KVVHVLWEGRFREAVDPGWAEEEVPNRRLARALFPAEELLEGPAMGRFHWSGIRAPHQ
                     KDPREETAAVWLDAGPRWTEGLLRLLILVEEAQGLLDRGTLDWFVLPWIDKFFISSLR
                     ELDHEYLGLLFDLLRDSGIDPLVLLWEDTARVQSPSLQIALDRLASRGYPFRGIGIFH
                     SGGVDLRDAPEIILEEQATVRLFALRPLFEGGRHRSFQELLGHPDPGFFREYNSSWKD
                     NLCFLYAGTQVRPLVSLQWETEPFPAWIALPGGKLPFGRFLRQRLRRQVPGKENGNPD
                     PFALDYARWANLL"
     gene            7698..7991
                     /locus_tag="PLM79_14980"
     CDS             7698..7991
                     /locus_tag="PLM79_14980"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOV87661.1"
                     /translation="MLGCWRRRNGCSAETAVSEACGAQPLSRAQAGRRLKVAGIAGGR
                     HVCARMAAMGIYPGVEMELLCAGCGSPCLVRVHGGTLSLGAGVSHKILVTPVA"
     gene            8117..8362
                     /locus_tag="PLM79_14985"
     CDS             8117..8362
                     /locus_tag="PLM79_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOV87662.1"
                     /translation="MPMIPMRKMLAGQEGVVTRVTARGELGRRIRDMGLVPGTPIYIQ
                     GRAPLKDPVAVRVRDFTLSLRNNEADFIEVEVADSHG"
     gene            8355..>9444
                     /locus_tag="PLM79_14990"
     CDS             8355..>9444
                     /locus_tag="PLM79_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOV87663.1"
                     /translation="MGEAPRPFLVALAGNPNCGKTTLFNAVTGARQHVGNYPGITVEK
                     KEGFCSFDGFPIRLVDLPGTYSLTAYSPEELVARDMLIHERPAAVINIVDASNLERNL
                     YLTIQLLELGVPLVLALNMVDAAANRGIEIDADELSRRLGVPVVPTIARSGKGKEEVL
                     SRAKDLAGQERPWVPPAISYGPDIDPILERMETLIEAAGFLSHLHPPRWTALKYLEAD
                     EQVMERGRETNPELSARLEHMAEAVGDHLQKTLATYPEAIIADHRYGYITALLKGSVV
                     RRSGKADRLYLSDQLDRVLTHRFLGPVVMMAILYGVYQFTFTYSAAPVAWMESAFSWL
                     GKTVEGVLPQGPLKSLVLSGVIDGVGGVL"
BASE COUNT         1824 a         2933 c         2928 g         1759 t
ORIGIN      
        1 gcgacttgtg agcccggacc tggaagatat tcagtccctc ggtgagcatg aggaccttgc
       61 cgggccgaag gaggttgaaa atggtgtgag cggccaggga agcgtgggac atcaccaggt
      121 cggccccggc cgtgtgcaga atgttgaagt tgcggtccag ggtggcccgg ctgaggatct
      181 ggacgtcggg cctgagcctg cggcagtata tggtcaggta gatgttcacg tcgtcgttat
      241 gggtggtgat gaatatggaa ggagtttgtc gaatccccgc cttgaccagg gtttccaggt
      301 ccgccgcgct gcccgggata atgttcgagc agcgttccgc aaccttgggg ttcttttcga
      361 cgatccggta gtccagcccc ttgtttgcca ggacctgggc ggcggcccga cccaccctgc
      421 ccccgcccag gatgagcgcc ggggcgtcgg ggaccgacct ttttccaacg aaatcatcat
      481 agcgtgccaa ctggtcttgg gaacccgcca acacgagaac tgtgttcgat tgaatggtgt
      541 cgctcagttt tgggatctgg aatactccct gctcccacca acccaccacg atcatgcccg
      601 tagcttcccg caggccgcaa ttgaggagcg tgcgccccac cagcggggag tgggctgcgg
      661 gggcctcgac aatcagcagt tctccaaagt gcccgatcat gttggagctt gttctcccgg
      721 ccagggtgcg gcgcgccagg aactccccca ggattttcgt gaactgaaaa acatgagtcg
      781 ccccggccag ctccaaaacg tccacggagt cttcctcctc tgcattggcc accaccggga
      841 cgtgcttgga aatttcgcga accgtgaaaa cgatgttggt gttcttcatg tcgtcttcga
      901 gggcgacgat catggcggca cgatccgccc gcaaccttcg gtaggtttcc gggtcatcgg
      961 gctcgcccac cacaacccgg tagccccggt cataaaggtc cagggccagg ttcacgtcgg
     1021 tcaccaggac cgcatgggga tgaccgtact gcttgagccg actgatgagg ctcatggcaa
     1081 tggggtcaaa gccgatgagg atcacgtgcc cggcttcgcc ctccggcaac tcgcggggtg
     1141 cgcgggactt ggactgggcc tccagccagg gggcatagaa gaactggatg aaggtgaagg
     1201 gcagcatgat caacagaaag acaatgcccg agaggagcac caggatggag aagacgcgac
     1261 ccaggtcgcc ggagaaggtg atgtctccaa agcccagggt ggacatcacc gtgagggtcc
     1321 aatacagtcc cgtgatccag gagaaatccc gcccttcgaa ttccatgatg aaatggaaca
     1381 ggatactgta gagaccaatg aagaagacca ggatcgccat gaaccggagg agagaccgga
     1441 tgtttctttt ggcccgaggg tcctggagca ggtacatgac ttgtgccgga acgaacttca
     1501 tgcgaacgtt ctccccagaa aggcgtttta cgcccgcgag gcgattctcg aatacctgga
     1561 tgacctcgag gacatttgcc tcgccgaaaa gcggctcgaa gacctccgag ggggccgtac
     1621 ccgaacctac accctggaag aggtggagcg ggaccttggc ctgggtgatt gagttccaag
     1681 agggagcgaa gaaggacttg gccaaactcg agacgcaggt cgcacgacgc attggaactt
     1741 ttcttcgtga ccgcgtcgcc aacctggacg agcctcgcag catcggacga gccctcaagg
     1801 gctcacggtt gggcgagttc tggaagtacc gggtcgggat taccgcatca tctgcaagat
     1861 ccaagacgaa gggctgatcg tcttggtagt ccggatcggg aatcggcgtg aaatctaccg
     1921 aatttgacgg ctcccctttc caaaattcca tccacggtac ttgccctacc tgttcgcccc
     1981 cgtaccgaac ccatccggat accgaaaatc tgctctccga ggtgcatgtc gcagccaacg
     2041 cccctccctc ggccgcctcg gacccctcaa ctcagggtgc gaaatcctgt ccgtctcagg
     2101 ccggaatctc cttttcgtta cctcactttt tgggccgcgt ccatggctgg attccacgag
     2161 acttgcaggg accggcaatt cccggagttg aacgggcagc ttggtgggac ctgaaatctc
     2221 gtgttgcgga gaaaaaaaag aacatcgata tagcatcgat gctcctctcg aactcgagcc
     2281 gcacgtatga cccgccagcg acggtgtacc gcccaggaat cggggcgcca taagccggct
     2341 gcttcgttat ggaattggtc ggggcgagag gatttgaacc tccgaccccc tgctcccaag
     2401 gcaggtgcgc taaccagtct gcgctacgcc ccgacaagga ccccgtttgg ttatcacagg
     2461 ccctcgggaa aagcaacgcg catttcccct ggtacggcgc gggaagacgg gaactgccgt
     2521 cggtcggctt ttccggggat cgacgaaatc ggagcggaga ttttctcccg gccgggtcgc
     2581 tctccgggaa aacgttctca cggcatcgcc gggtcgagcg gaagccgacc cacggcggca
     2641 actttcggtc gggcgggccg tccggcggac cattgcggcc catgggcaaa ggaagatctc
     2701 tctccccaac ggggagaagg gcgagtgagc ggtgcggtcg gatatttcta caacagccct
     2761 tttcccggcc gggtcgagcg gagcttctcc cttccctcga tcccatggaa agggccgaag
     2821 aagggggcgc ggatctcgtt tggatccgtt ctcagcccgt ttcccggtct gcggtggggc
     2881 ggcccccgac ggtccttcgc tgcagcttcg cctggcaaag ttccgcatag gccgcgtcga
     2941 gcagttcctg gggacgccgg gtgtgctcct gcatcgaccc cactccaatg tgggcatgga
     3001 tgaggatggc ggcgttttcc agcttgaagg gcgacttgcg gatggtttga gccaggtacg
     3061 gaatcccctc ccgggcttcc tccccgggaa cccggggcag caccgcaaga aactcgtcgc
     3121 tcccgaaacg gcccaggaac gcttccccct ggggcagggt ggccttcaac aggttcgcca
     3181 catgggtcag gattctccct ccgatgtcca accttccttc ttcgtcctgg aaaaaatcca
     3241 ggtcgatgat caggaccgaa agcggttcct cgagtcttcg ggccctgcga aactcccttt
     3301 cgaggaccgt gtggatctgg aagaaattga gcagtcccgt caccgggtcc tgaatggaga
     3361 aatccttgac cttttcgtag gtcaggcagt tttccatgca aagggcgatc ttgattccca
     3421 gctgttccag gaggtccgtc ccgtcgtcgg gtcggtagcg ttccgcgtcc aggcttccca
     3481 ggaacaggaa cccgaagtgg acctcgtgga cgcaaagggg aatgaggacc cccgactgca
     3541 ccggctccgg ggaatccggg agatactccc cgagcttgcg gagctcttcc ccgctgagca
     3601 gcgccggctt atggggatcg cggcagacga aaacccctac cgccgccggg cacggcacaa
     3661 tccaggtgcc gtccccgatg ggctcggagt cccccgagag ggccgggaaa tactcctgga
     3721 tcaagtcctg gtggcagaga agaagagtga cttggtccgg ttcgaagcga tccttgattt
     3781 cgaggagaag ttcctccacc agttggttca ggtcccgagt gcgaaagagg attctttcga
     3841 tttccgtaaa atgcctccag atcctctcgt tttccgccgc agcttccagg accccgttca
     3901 gtctcttgcg caactcggcg ttttccgcct cgaggccgga ttccgcctcc ccgggccgag
     3961 cttccccctc tccggcgtcg ccgggaaggg ggtcggggtg atctaacctc cgatccacag
     4021 gttccatgaa cgaagcctca ccatccggtt tgagcatccc aaagccgcgg gcaacgcgcc
     4081 ctgacaaacg aaattgcctc ctccaccgcc tctttgggct gcttcaccac caccagcccg
     4141 gggacggaga acctaggctc gagggcgatg accggtttgc cgatcttcag ggccatggcc
     4201 atctccgaca gggttccata ttccccgtcc acggcaatga gggcgtccgc cgtgtgcacg
     4261 atcacggcat ttctggcctg tcccatgttg gtggcaacgg gaatgtcgat gtagcggttg
     4321 gcgtcatccg gcccggctcc gggcaggatc cccagggtga ttccacccgc ttccttggct
     4381 ccccgggccg cggcttccat gacgcctccc agcccgccgc acaccaggat cgcgccgcac
     4441 cgggcgatct gccgaccgac ctcgagggcc aattcttcga ggccgggggc ggtcttcccc
     4501 gttccaatga ctcccaggac aggccttctc atggactcct aacctatacc gagcgattct
     4561 tgagggtcat atcccgtcca acgacgctcc cggcgggcgg gaacggggcc ccgcccccac
     4621 aggacggttc gcctgcaggc ccgggggcgg gcttgctgca catccctaca acgtggaaaa
     4681 tggctcattt ggggtcctac tcttcccacc cccattttcc cacgaggtcc acaaagcgca
     4741 ctccccccag gttgtgcttc cgaattccct cgggtgcccg ttccaccacg atgagttcct
     4801 gggaaatgcg gtcccccacg gggatcacca gtcttccctt cacggccaac tgatccacca
     4861 gggtttgggg gaccttgggt gctccggccg tgaccacgat gccgtcgaaa ggactttcct
     4921 cgggccagcc cagggtgccg tcccccaccc gcacgatcac gttcctgtag cccagtttct
     4981 ggagcacctg cccggcacgg tgagccaggg atgcgaatcg ttccatggaa aagacccggt
     5041 gggccagctc ggcaagcacg gccgcctggt acccggaacc ggtgccgatc tcgaggacct
     5101 tctcgtgccc cttgaggtgc agggcttcgg tcatgagagc gacgatgtag ggctgggaga
     5161 tggtctgctt gtccgaaatg ggcaaagggt ggtcgttgta ggcctgctcc tggagggcct
     5221 cgtccacgaa caggtggcgg ggcacctttc gcatggcgat caagacgcga tcgtcgtcga
     5281 tgccgcggcc gatgagctgg gtttccacca tccgatcgcg cgctttttgg aaattgatca
     5341 tgagcgaacc ctggttgtgt cacacatccg ccacgggatc cgtcggccgc cgtttcatcc
     5401 gttagagcta atgacaaagc aaaatggttg tcaatggctt tttgcaggct ttgtgcaccc
     5461 ttggaccgtc aaagcaggtt ggcccagcgc gcgtaatcga gagcgaaagg gtccgggttg
     5521 ccgttttcct ttcccgggac ttgtcgtcgc agtcgctgac ggaggaagcg gccgaaggga
     5581 agctttcccc ccgggagtgc gatccatgcg ggaaaaggct ccgtttccca ctgcaaggaa
     5641 accaggggcc gaacctgggt gcccgcgtag agaaaacaaa ggttgtcctt ccaggacgaa
     5701 ttgtattcac ggaagaaacc cgggtccggg tgccccagga gttcctggaa ggaacggtgc
     5761 cggcccccct cgaaaagggg tcgcagagcg aagagccgca ccgtcgcttg ctcttcgagg
     5821 atgatttccg gggcgtcccg aagatcaacc cctcccgaat ggaaaatccc gattccccga
     5881 aacggatacc cccggcttgc caggcgatcc agggcgattt gaaggctggg gctttggacc
     5941 cgggccgtat cttcccagag gagcaccagg ggatcgattc ccgagtcccg gagcaggtca
     6001 aagagcaggc ccaggtactc gtgatccagc tccctgaggg aggagatgaa aaacttgtcg
     6061 atccagggca ggacaaacca gtcgagggtt ccccggtcca acaggccttg ggcttcctcc
     6121 accaggatca agagccgcag gagaccctcc gtccaccgag ggcctgcatc gagccaaacc
     6181 gccgccgtct cctcccgggg atccttctgg tgaggggccc ggatgccgct ccagtgaaat
     6241 ctccccatgg ccggaccttc caacagctcc tctgcaggga aaagcgcccg ggcgaggcgt
     6301 cggttcggca cctcctcttc ggcccagccg ggatcgacgg cctcccggaa ccgtccctcc
     6361 cacaaaacat ggacgacctt gggcgccagc aatcgcttct ttcgctcctc ccaaaggtct
     6421 gccatccggg agagccgggc cgcatcccga ccccgcgcat cccggagcct ccctttcacc
     6481 ctctttttga aatcttccca tgcgcatcgg gaaggaaacc tctcctcgat gctttcgaag
     6541 gcccagcctc cggcggcggc cagggtggcg ttgtgccagc tgagcaccac ctgccgggag
     6601 cccagactta cggttcgggc caggtccctt atcaaggcca gttcccgctc ctggcagcga
     6661 atcaagccgt ccagaaagga gcgaagtcga tccccgcaac ccgcccagta ggactgccgg
     6721 aggccgggaa agctcccccg gatcttttca ttgcctgcag gagccatgag cacgggtcgg
     6781 ccgatgcgca gccagcgacc ggacgggacc ggtcttccgc gccggggggc cttctttctc
     6841 cagaggggga gtaagtcccg gcaagtgggt tccgcccagg gcggggaacc ggtccggcga
     6901 acccggtttc tcaaaagcct ccaccgggtc cgttggaccg agacggacct gccggtcccg
     6961 ggaaagaaaa agacgggcgg caggaaatcc gcccacagtc ccaggggaac gggacgatgg
     7021 aaaaggcatg aaaaaaaggc cccggaaggt ttgttcaaga aatctcttac cggatcccgg
     7081 gctttggtcc tcggcccggg ccgatgcacc agggccatcc agtcgcagag gacccgccac
     7141 aggataggcg gcaggagacg ggattcggga cccagggagg aaaccgcctc caggaggtgc
     7201 ccgtaggaac gcggctcccc atcttccagg aacaggagga cccggttcaa ggcctcctcc
     7261 ctcacggctt tccggggatg accgcacagg agcaaatgat ctctgagggt tcggcaccag
     7321 gagggtcctt gggctcccgg gccgggggtg tcgtaggtgg gaaactccga gctgcctttc
     7381 atgggagact cccttcaggc cccgggcatc gcggacgcag gcggcgcgtt cccgcgagcc
     7441 tgcggttcgg aaaggaaaca agccgccgca ggggggtgtt cggagaaaag acatgggcgc
     7501 ccggagctgc gggtctcatg aaatcagcgt tttcatcccc ttacggaaga accttgtccg
     7561 cccggacctg ccgggcgaga ttggagggac cctccagagc atagggacca tgggaaaaaa
     7621 cgcaagaaag atgttgactg cgtataaagt ttggtttacc taattatcca acgcggagga
     7681 attccaagga gcagggtatg ctggggtgct ggagacgtag aaacggatgt tcggccgaga
     7741 ccgccgtatc ggaagcatgt ggagctcaac ccctttctcg tgctcaagcc ggaagaaggc
     7801 tcaaggtggc ggggatcgcc ggaggtcggc acgtgtgtgc ccgaatggcc gccatgggca
     7861 tttacccggg ggtcgaaatg gagcttcttt gtgcgggttg cggatctccc tgcctggttc
     7921 gggtgcacgg cgggacccta agcctgggcg cgggggtttc ccacaagatc ctggtgactc
     7981 cggtcgcttg atcgaagcca caaaatcatg gatggacgga agcggctgag gatacgggca
     8041 gtcgcttttt tccgggcggt tcgggcaagc tctgcttgcc cgcggtgttt ttgagacaga
     8101 gacgggtcat tctcccatgc ccatgatccc catgcgaaag atgctggcag gccaggaagg
     8161 agtggtcacc cgagtgaccg ctcggggcga actgggacga cgaattcggg acatgggcct
     8221 tgtgcccggg acccccattt atatccaggg tcgcgccccc ctcaaagacc ccgtggccgt
     8281 tcgggttcgg gacttcaccc tgagcctgcg caacaacgag gcggatttca tcgaggtcga
     8341 ggtggcggac agccatgggt gaggcgcctc gaccattcct ggttgccctg gccgggaacc
     8401 ccaactgcgg caagaccacc ctgttcaatg ccgtgaccgg cgcgcggcag cacgtgggga
     8461 actatcccgg catcaccgtc gagaaaaagg aaggcttttg ttccttcgac ggctttccaa
     8521 ttcggctggt ggaccttccg gggacctact ccctcacagc ctattccccc gaagagcttg
     8581 tggcccggga tatgctgatc cacgaaaggc ctgcggcggt catcaacatc gtggacgctt
     8641 ccaaccttga aagaaacctt tatctgacca tccagctgct ggagttgggg gtccccctgg
     8701 tgctcgccct gaacatggtc gacgccgccg ccaatcgcgg catcgaaatc gatgcggacg
     8761 agttgtcccg gaggctgggc gtcccggtgg tccccaccat tgcccgatcg ggaaaaggca
     8821 aagaagaggt cctttcccgg gccaaggacc tggccgggca ggagcgtccg tgggtccccc
     8881 cggcaatctc ctacggaccg gacatcgatc ccatcctcga gcgaatggaa accctcatcg
     8941 aggccgcagg atttctttcg cacctgcacc cccctcgctg gacggccttg aagtacctgg
     9001 aagccgacga gcaggtgatg gagcggggcc gggagacgaa ccccgagctc tcggcccgcc
     9061 tcgagcacat ggccgaagcc gtcggggatc acctgcaaaa gacgctggcc acctacccgg
     9121 aggccatcat cgccgatcac cgttacgggt acatcacggc cctgctcaaa ggctccgtgg
     9181 taagacggag cgggaaggcc gatcgcctgt acctttcgga ccagctcgac cgggtgctga
     9241 cccaccggtt cctgggtccc gtggtcatga tggcgattct ctacggagtc taccagttta
     9301 ctttcactta cagtgccgcc cccgtggcct ggatggagtc cgctttctcg tggctgggca
     9361 agaccgtgga gggtgttttg ccccagggcc cgctcaagtc cctggtcctc tcgggcgtca
     9421 ttgacggggt cgggggagtc ttgg
//
LOCUS       DAOOVC010000150         9308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4633_length_9308_cov_6.024425, whole genome shotgun sequence.
ACCESSION   DAOOVC010000150 DAOOVC010000000
VERSION     DAOOVC010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9308
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_9308_cov_6.024425"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1048..1461
                     /locus_tag="PLM79_14995"
     CDS             1048..1461
                     /locus_tag="PLM79_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87664.1"
                     /translation="MSSGRSVLIIEDDKDLVSAMKVVFESKGFSVKTAYNGKDGADAV
                     RKQKPDAIVLDVMMATLTDGFDLAHYLKDTPEFKDIPIIMVSGFPQEMAKLGPESFQS
                     ALQDDWPAAKFLEKPVDPEKVVAAVESILKEAWKP"
     gene            1615..3462
                     /locus_tag="PLM79_15000"
     CDS             1615..3462
                     /locus_tag="PLM79_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOV87665.1"
                     /translation="MRPGYELVKEQIFFRKELRERVLWAIKIRWVFAVLALAGLAVAA
                     PMGLDLPVFPLAAVCAAVLLYNAAFARAARWIEGIDPSEVRPFTLFAHCQISCDLAAV
                     FVAVWLSGGMSSPFLIFALFHIILAGILLPPASTFAYVGAALLLTAVTALAQQLPQLG
                     LPPVSPRLPTCELPGHLGSVVFQYLIFAGAVVFSAFLTTSIRAGLRTKGRNLLRISKE
                     LEASNARLIALYEMVKEIGRHSGLKELLDSATRHAANLMGVRACSIKLLDEQKRYLEF
                     AATYGLSEDYLSQGKLDLSKSPINREIIEGKSHVIGRIDEKDYFQYPENIEREGISSM
                     LCLPLRGNNMTLGVFCIYGDRYYLFGEDDVEFFGLMSDLTGIAIERVKWDLTKSWFMA
                     KAAHNLRAPIGAVLSMVKLVRKGYLGPVNDKQAETLERCEVRMGMLADLISDLLRIGK
                     ERTEMGKSELRPVAAEEILGKLEPMFRDQALQKGIEMNVETRGSGLWVLADANLLDDL
                     FANLISNAVKYTPPGGTVKVFLGMEGDNQVRFEVADTGIGISKEDLPKLFSEFFRTEA
                     AKELVEEGTGLGLVIVKEILDRLGGKIHVESEVGKGTRMSCLIPASRPA"
     gene            3749..3904
                     /locus_tag="PLM79_15005"
     CDS             3749..3904
                     /locus_tag="PLM79_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87666.1"
                     /translation="MGEMTRAMEEVSRSGKEEARRRAVFALWAHGLAGRAPLCYAGKR
                     LHRRVPE"
     gene            complement(4091..5296)
                     /locus_tag="PLM79_15010"
     CDS             complement(4091..5296)
                     /locus_tag="PLM79_15010"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOV87667.1"
                     /translation="MTSRTRTRLIFAFLLFFLDSTALWAGDAEDPRRETSPPQARSII
                     LMVADGLGMANVTAARIHKNGPDGPPLFLELPHVGYQRTYGADGIVTDSAAAASAWAC
                     GEKFNHGEVCLHADSKEPPKSILELAGMKGKATGLVATSTITHATPAAFASHVASRKC
                     ETEIARQYLRITRPDVLLGGGWSRFAPAQPDPCGASGDLLAEARARGYSVVHTESDLR
                     GAVERGASRILGLFAGEAMTPENRRTAESFEPPLWEMTASALKTLGRNPRGFFLLVEG
                     SQIDHANHRKDLPYLLGEVLGFDRAVKTVLDWLEEQPSRKDSTLLVVVSDHETGGFAI
                     HGPRKGLTAKGGSIEPGWSFTGGDTSQKVGHTGVDTPVWSLGPGSSLLNRPAMENTEI
                     YRVMKSVLD"
     gene            complement(5360..6193)
                     /locus_tag="PLM79_15015"
     CDS             complement(5360..6193)
                     /locus_tag="PLM79_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOV87668.1"
                     /translation="MIRGNYHTHCSFCDGALEPEAFVAEALRQGMDPLGFSSHAPLPF
                     PNTFTMKMEQVPDYLRILRELRERYRDSIRVLAGLEIDFVPGIEEYQEGFYSLGLDFT
                     IGSVHYVDADEQGTPWPVDAEPELFDRGIVEVFRGNVRAAVERYYQLVREMVGTNRHT
                     LVGHLDVIKKNNPNGRYFSEDEPWYRDAVEETLEAIVRSPAILEVSTGGIRKNVGIYP
                     GVPILKRCRERGVRITLCSDAHHPDHLSAGYPGVEQLLRDLGFKEIFNHTGNGWEPCP
                     L"
     gene            6781..7773
                     /locus_tag="PLM79_15020"
     CDS             6781..7773
                     /locus_tag="PLM79_15020"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOV87669.1"
                     /translation="MPGEIKRADAVFEGGGVKGIGLVGAVAVTEEKGYRFENVAGTSA
                     GAIVAALIAAGYKAGKLKEIIEGLDYNDFKDKGMLDKIPIIGFALSLGFEKGIYEGDF
                     FEKWLRELLLKASPPVRTFKDLRTGEQDPRYRYKLQVIAADVTRGMMLVLPRDIEDYG
                     IEPDDLDVALAVRMSMSIPFFFEPVILKDKHGLDCYIVDGGVLSNFPVWLFDDKTADP
                     PWPTFGYKLVEPDSGKPNRINGPITLFAALFNTMMDAHDARYIEDADFVRTIPIPTLG
                     VRTTEFDLSRKRSDNLYQSGRGAAEKFFEHWDFANYKKEYREKEAMGRRARVSA"
     gene            8397..>9308
                     /locus_tag="PLM79_15025"
     CDS             8397..>9308
                     /locus_tag="PLM79_15025"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOV87670.1"
                     /translation="MRLTEELEKEYGTLFDTCRVRDENRREVDETVKKILRNRHRYEG
                     VGAQTGVPWYVIAVIHSLECGLKFDRHLHNGDPLSKKTRNVPQGRPPGDPPFTWEFSA
                     RDALTYDDLTRWTHWGLAGTLYRLEAYNGWGYRRNDYGVLSPYLWSYSNHYTRGKFVR
                     DRVFDPEARSKQCGAAVLLKALEEIGAVEFAETPPKRPAEKGRPAYPGKILKQGEKDS
                     ESVRMLQLRLNELGCGPIDPDGDFGSITAAAVRLFQARSVGNDGSPLKMDGEVGPLTW
                     EALFGRRTLTRFVPSASGDPLLVKAIEV"
BASE COUNT         1815 a         2732 c         2972 g         1789 t
ORIGIN      
        1 tgatgtccag aagacagtgg tgtcgggaca ccacacgatg ggcggcgtcg ttgggcagcc
       61 gcaggttgca gtcccgggcg cgcccgatga tgcaggtgga gcgctcctcg aagcggtatt
      121 cgcaacctcg ggtgggccct tccacgactc tcaggctgac tcgtgcaggc atgacgttct
      181 ccctgggctt tatccgatat tggatcagag tatacttcgg cattttcatg gatttggaaa
      241 gaaaaaaagt aggcgagaga ccggtccctg agggcagcct ccacccgggg ctcccggatt
      301 cgggccccgt tcatgagcct caagggaagg gaccgccggt tccatggtcc cgacggtttc
      361 cgggggggcg cggcaggaac ccggccggtt cgcttgttgt tgacttgacc ggatgccttc
      421 agtagtattg aagactcgca agctctaccg aaaggtcgtg tcgtctttac cgggtttgca
      481 tcctgcattc gctcgaacgg cgtttggact atgttctcga aagccggtgt tcttccatgc
      541 ccgaatcccc cgacaagagt cggggtgccc agttttttgc atctttgcct tggtccaaga
      601 agtaccccta tggttttttg atccatcatc ggccgggtgt ggttccggtg tttcattccg
      661 aatcgttgcg gcaataccca ataatcgagc gattccgggg acgacggggc gagcacgaac
      721 acgaggctct gcggagggag acggactgtg ccgtcgcccg acggattcct cggcctttgg
      781 ccggggccgc ctcccgcccg cccgaatcga tgccggcagg gatggactcc ctggacctgc
      841 acgggtcggg gagagcgagt ttaccctcgt agattgcctc ggcggaatca aagtcctgaa
      901 tccccgggtc atgatttttg tagccccgca gaaaggaggg acgcccggag cgttcatgca
      961 tgacggagcg gttgcatcgt tggtcttcaa ttcttgacgc gatcccatgg atcggtaatg
     1021 cggattcaaa cagacaagga gaaagtcatg agcagtggac gcagcgtgtt gatcatcgaa
     1081 gatgacaagg acctggtgag tgccatgaag gtggttttcg aaagtaaggg tttctccgtg
     1141 aagacggcct acaacggcaa ggatggagcc gacgccgtgc ggaagcagaa acccgacgcc
     1201 attgtcctgg atgtcatgat ggccacgctc accgacgggt tcgacctggc ccattatttg
     1261 aaagacaccc ctgaattcaa ggacattccc atcatcatgg tgagcggctt cccgcaggag
     1321 atggcaaagc tgggaccgga atccttccag agtgcgctcc aggacgactg gcctgcggcc
     1381 aagtttctgg aaaagcccgt ggacccggaa aaggtcgtcg ccgccgtgga aagcattctc
     1441 aaggaggcat ggaagccgta agactatgcc cacgtcccgc acgcccctgg gccgggttcg
     1501 ttgaagccgg ggcccagggc gtcgggggcg cggggacctt tgttcgaagg tcggggcccc
     1561 gttcctgtcc aatcccactg ctcgtccatg cgaaccagcc ttcatgggga ccttatgcga
     1621 ccgggatatg agctcgtcaa ggaacagatc ttttttcgca aggagctgcg cgagcgggtc
     1681 ctatgggcca tcaaaattcg atgggttttt gccgttcttg cgttggcggg cctggccgtc
     1741 gcagccccca tgggcctcga tctgccggtg tttcccctgg cggcggtgtg cgccgccgtg
     1801 cttctctaca atgccgcctt cgcccgggcg gccaggtgga ttgaagggat cgacccgtcc
     1861 gaggttcgac ctttcaccct ctttgcccac tgccaaattt cgtgcgacct ggcggcggtg
     1921 tttgtggccg tgtggctttc cggcggaatg tcgagccctt ttctgatctt tgcgcttttc
     1981 cacatcatcc tggcggggat tttgctccct cccgcctcga cctttgccta tgtcggtgca
     2041 gccctgcttc tcacggccgt gacggccctg gcccagcagt tgcctcagct gggattgccc
     2101 cccgtttccc cccggcttcc cacctgtgag ctcccgggac acctgggctc cgtagttttc
     2161 cagtacctga tcttcgcggg ggccgtggta ttttcggcct tcctcaccac ctccatccgg
     2221 gccggcctgc gcaccaaggg tcgcaacctc ctccggatct ccaaggaact cgaagccagc
     2281 aatgcccggt tgatcgccct ctacgagatg gtcaaggaga tcggcaggca ctcgggtctc
     2341 aaagaactcc tggattccgc cacccgccat gcggcgaacc tcatgggggt cagggcctgt
     2401 tccatcaaac tcctggacga gcaaaagagg tatctcgagt ttgccgccac ctacggcctg
     2461 agcgaggact acctgtccca aggaaagttg gacttgagca agagccccat caaccgggag
     2521 atcatcgagg gaaagtccca tgtgatcggg aggatcgacg agaaagacta ttttcagtac
     2581 ccggaaaaca tcgagcggga ggggatctcg tccatgcttt gccttcccct gcggggaaat
     2641 aacatgaccc tgggtgtatt ttgcatctac ggggaccggt attacctctt tggagaggac
     2701 gatgtggaat tcttcgggct catgagcgac ctgacgggca tcgccatcga gagggtcaag
     2761 tgggacctga ccaagtcctg gttcatggcc aaggcggccc ataaccttcg agctcccatc
     2821 ggggcggtcc tcagcatggt gaagctggtt cgaaaaggct acctgggtcc cgtgaacgac
     2881 aaacaggccg agaccctgga gcggtgcgaa gtccgaatgg gaatgctggc cgacctcatc
     2941 agcgacctgc tgcgcatcgg caaggaacga acggagatgg ggaagagcga gctgcggccg
     3001 gtggccgccg aggaaatcct gggaaagctc gaacccatgt ttcgggacca ggccctgcaa
     3061 aagggcatcg agatgaacgt cgagacccgc gggtcggggt tgtgggtcct ggccgatgcg
     3121 aacctcctgg acgacctttt tgccaacctg atctccaacg ccgtcaagta caccccgccg
     3181 ggcgggacgg tgaaggtgtt cctgggcatg gagggggaca accaggttcg gttcgaagtc
     3241 gccgatacgg ggatcgggat ttcgaaagag gacctcccca agctcttttc cgagtttttc
     3301 cgcaccgagg ccgccaagga gctggtggag gaggggaccg gcctgggcct ggtcattgtc
     3361 aaggaaatcc tcgatcggtt ggggggaaag atccacgtgg agagcgaagt aggaaaagga
     3421 acccgcatga gctgtctcat ccccgcatcc cggcccgcat gagcccgcaa gacccgtggg
     3481 ccgtcaagtc ccaaggggcg tgatcgtccg ttccccgaac cattcggcag tcccccggct
     3541 caaggcagcg tcgttgagca gaattcagtc ctggtgcgac ccacagacct ggagaaatcg
     3601 tcaccacgtg tccccgcgcg cccgcttggc gagttcgtta gcggatatgc gcactgaggg
     3661 gatccgggaa tccagtcaag gaggagaaac cgttgttttc cctcggatgg cggaagcaca
     3721 acagggcgtc caaagagtca gcgggcacat gggcgaaatg acccgggcca tggaagaggt
     3781 ctccagatcc ggtaaagaag aagcccgcag aagggcggtt ttcgcccttt gggcccacgg
     3841 gcttgccggg agagctcccc tgtgttacgc gggcaaacgg ctccaccgaa gggtcccaga
     3901 ataatccgtt gtatttcgcg atgcacaatg cccggcgggt gtttcctaag ggcgcggttt
     3961 gcgccgatca aagagaccat tggaggggac tccgggacct gtggcgtgcg gtgttgaagg
     4021 tctttgccgg gtggggaacc cgagggacgt gccgtgacgg tgccgggggc aggactccgc
     4081 gggcagggcc tcagtccagg acgcttttca tcacccggta gatctcggtg ttttccatgg
     4141 cgggacggtt caacaggctg cttccggggc ccaggctcca aacgggggtg tccactcccg
     4201 tgtggcccac cttctgggag gtgtctcccc cggtgaagct ccatcccggc tcgatcgagc
     4261 cgcccttggc cgtaagcccc ttcctcgggc cgtggatggc gaaaccgccc gtttcatgat
     4321 cggatacgac cacgagcaac gtcgagtctt tccgggacgg ctgttcctcc agccagtcca
     4381 ggaccgtctt caccgcccgg tcgaagccca ggacctcgcc caagaggtag gggaggtcct
     4441 tccggtggtt ggcatggtcg atttgggagc cttcgaccag gaggaaaaac ccccgggggt
     4501 ttcgtcccaa ggttttcaag gccgaagccg tcatctccca aagcgggggc tcaaagcttt
     4561 cggctgtccg gcgattttcg ggggtcatgg cctccccggc aaagaggccc aagatccggg
     4621 aagccccgcg ttccaccgct ccccgcaggt cgctttccgt gtgcacgacg ctgtagcccc
     4681 gtgcccgtgc ctcggcgaga aggtcccccg acgctccgca aggatcgggt tgcgcgggag
     4741 cgaaccggct ccagccgcct cccagcagta cgtccggtcg ggtgatccgg aggtattggc
     4801 gggcgatttc cgtttcacac ttgcgggagg caacatggga agcaaaggcg gcaggggttg
     4861 cgtgggtgat ggtggaggtg gctacgagcc ccgtggcttt gcccttcatc ccggccaatt
     4921 cgaggatgga cttgggaggt tccttggaat ccgcgtggag gcaaacctct ccatggttga
     4981 acttctcgcc gcaggcccag gccgaggccg ccgccgccga atccgtgacg atgccgtcgg
     5041 cgccgtaggt gcgctggtac cccacgtggg gcaactcgag gaagagaggg ggaccgtcgg
     5101 ggccgttctt gtggatgcgg gctgcggtga cattggccat gcccagtccg tcggccacca
     5161 tgaggatgat gctgcgggcc tggggagggg aagtctcccg gcgggggtct tcggcatccc
     5221 cggcccacaa tgccgtcgaa tcaaggaaga aaagcaggaa ggcgaagatc agccgggttc
     5281 gggttcttga cgtcatgcag gctcctcgaa aaggagagaa agaggggcaa atcgagattc
     5341 ggcatgaact ggaccggggt cacaacggac acggttccca cccgttgccc gtgtggttga
     5401 agatctcctt gaagccgagg tctcggagca gctgttccac ccccggatac ccggcggaca
     5461 ggtgatcggg gtggtgagcg tccgagcaga gggtgatgcg gactccccgt tccctgcagc
     5521 gcttgagaat gggaacgccg gggtagattc ccacgttctt tcgaatcccc ccggtgctga
     5581 cttcgaggat ggccgggctt cgaacgatgg cctcgagggt ctcttccacg gcgtcccggt
     5641 accagggttc gtcctcggaa aaataccggc cgttcgggtt gttcttcttg atcacgtcca
     5701 ggtggcccac cagggtgtgc cggttggtgc ccaccatctc tcggaccagt tgatagtatc
     5761 gttccacggc ggcacgaacg tttccccgga aaacctcgac gatcccccgg tcgaagagtt
     5821 ccggttccgc atccacgggc cagggagttc cctgctcgtc tgcgtccacg taatgaacgg
     5881 agccgatggt gaagtccagt cccagggagt agaacccctc ctggtattcc tcgatgcccg
     5941 gcacaaagtc gatctcgagc cccgccagga cccggatgga atcgcggtat cgttcgcgaa
     6001 gttcccggag aatccgaagg tagtcgggaa cctgctccat cttcatggtg aaagtgttgg
     6061 ggaagggaag gggggcatgg ctcgagaagc ccaaagggtc catcccctgg cgaagggcct
     6121 cggcgacaaa ggcttcgggt tctagggcgc cgtcgcaaaa ggagcaatga gtgtggtagt
     6181 ttcctcgaat catgaaccgg caggggcctt tctggattgt agtcgaagtc tggacccgga
     6241 tctcaaggga gaaagggtcg gccgttggct tccgcacgaa cctggagcac tcatggtatc
     6301 caaaatgcgg cgcggggtcg agaggggaat ggattccgcc caaaaaccgg actcgagttc
     6361 ccccgatcct tgcccgggac atggccggga gcggcggcca tcgccccttc ctctttcttc
     6421 cttgatccct tggttccttt tctgtataag ggattcatct ttccgacaaa aagcagtgtc
     6481 ctatgggtga aaccggatcc tcctgatctt gtcttccaac gggcgttttg gcgcccaaag
     6541 gcccgggttt gcacgcgcgg gatcgccggt tgtgcggttc ttcccgcagg ctccgaacga
     6601 ctccggagaa acggctgtcc gctcctgtgc ccggggtaga tcctcatccc ggcgtgcccc
     6661 ggattcggtc cggtccggtc gccggaatcc gtgcgaagtg tttgaatccc cacttcggca
     6721 ggcagggcgg cgatcgtgcg ccgttctatg gaaccgtaca cgggatttta aggaggagtc
     6781 atgcccggcg aaatcaagag agcggatgcc gtgttcgaag gaggcggagt caaggggatc
     6841 ggcctggtgg gggccgtcgc cgtgaccgag gagaaagggt atcgattcga gaacgtggcc
     6901 gggacctcgg cgggagccat cgtcgccgct ctcatcgccg caggctacaa ggcaggcaag
     6961 ctcaaggaaa tcattgaagg cctggactac aacgatttca aagacaaggg aatgttggac
     7021 aagatcccca tcatcggttt cgctctcagc ctggggttcg agaaaggaat ctacgaggga
     7081 gatttcttcg aaaaatggtt gagagagctg ctgctcaagg cctcgccgcc cgttcgaacc
     7141 ttcaaggact tgcgaacggg ggagcaggac ccccggtatc gctacaagct ccaggtcatt
     7201 gccgcggacg ttacccgggg aatgatgctg gtcctccccc gggatatcga ggactacggc
     7261 atcgaacccg acgatctcga tgtggccttg gcggttcgaa tgagcatgag cattccgttc
     7321 ttcttcgagc cggtgatcct caaggacaag cacggcctcg attgctacat cgtggacggc
     7381 ggggtcctga gcaactttcc cgtatggctg ttcgacgaca agacggcgga tcccccctgg
     7441 cccaccttcg gctacaaact ggtggagccc gattccggaa aacccaatcg catcaacggg
     7501 cccatcaccc tgtttgccgc cctgttcaac accatgatgg acgctcacga cgcacgttac
     7561 atcgaggacg cggactttgt gcgcaccatc cccatcccca ccctgggagt gaggaccacc
     7621 gaatttgatc tttcccggaa gcgaagcgac aacctgtatc aatccggccg cggggcggcg
     7681 gagaagtttt tcgaacactg ggacttcgcc aactacaaga aagagtatcg ggaaaaggaa
     7741 gcgatgggtc gccgggcgag agtctcggcc tagacccgcc ctcttgtatc tgcgatcagg
     7801 cggggataga tcgccgcctc cgagcgaacc cggaaggttc gcaaccattg gtcgggggtc
     7861 gaggttgcaa ggccgatccc cccgaaacag gtccctatgc ggcgttgacc cgtggcgcga
     7921 agcttgcgga gccgaaggcg catgcaagcg agtgacatcg ggtccaatgg cctggttggc
     7981 agagccggag gcggagccgg ccgggacagg gacccgatgg gggcaacgcc gcattggcga
     8041 cgccgcaggc ggcgccaaca ggaggttgac ccatggggac ggaccgtccc gtgaacaagg
     8101 gtgggattgc gccttacacc ctctgaaccc gcgggacgtg ccgtacggag acccggctcc
     8161 tgaggtcggc catacggaga cctgacccct agtgccgttc cgggaatacc cccttcgagg
     8221 aaagtcgggg gatgtagggg gggcgtaagc gcatcccaat ccctaccccc caccccgaag
     8281 gggaaatcaa accggttgac aatgaaacgg catcgaggga gggaccccct ttccccgccg
     8341 ggaggaggtt ggggcagggg ctgctttcct tggtcgcaag aaagatcgga gacgtcatga
     8401 gactcaccga agagctcgag aaagaatacg ggaccctgtt cgatacctgc cgggtgcggg
     8461 acgagaaccg acgggaggtc gacgaaacgg tcaagaagat ccttcggaac cgacatcggt
     8521 acgaaggggt cggtgcccag acgggggttc cgtggtacgt gattgccgtc attcacagcc
     8581 tcgaatgcgg tctaaagttt gacagacatt tgcacaacgg cgatcccctt tcgaaaaaga
     8641 ccaggaacgt gccccaggga cgtccccccg gagatccgcc cttcacctgg gagttttcgg
     8701 cccgggacgc cctcacttac gacgatctca cccggtggac ccactggggc cttgccggga
     8761 ccctttatcg cctcgaggca tacaacggat ggggctatcg caggaacgac tacggcgtcc
     8821 tttcccccta cctgtggtct tactcgaatc attacacccg ggggaaattc gttcgggacc
     8881 gggttttcga tcccgaagcc cgttcgaaac aatgcggggc cgccgttctg ctcaaggccc
     8941 tcgaggaaat tggggcggtg gaattcgcgg aaactcctcc aaaacgcccc gccgaaaaag
     9001 gtcgtccggc ctatccgggc aaaatcctta agcagggaga aaaagacagc gaatcggtgc
     9061 gaatgttgca gctgcgcctc aacgaactgg gctgcggtcc catcgatccg gacggggatt
     9121 tcggaagcat caccgcagcc gccgtgcgtc tcttccaggc ccgatcggtg ggaaacgacg
     9181 gaagccccct gaagatggac ggtgaggtgg gacccctgac ctgggaggcc ctcttcggga
     9241 gaaggaccct gactcgattt gtcccttccg cctcgggaga tcctttactg gtgaaggcca
     9301 tcgaggtt
//
LOCUS       DAOOVC010000151         9271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4655_length_9271_cov_6.676758, whole genome shotgun sequence.
ACCESSION   DAOOVC010000151 DAOOVC010000000
VERSION     DAOOVC010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4655_length_9271_cov_6.676758"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..1587)
                     /locus_tag="PLM79_15030"
     CDS             complement(124..1587)
                     /locus_tag="PLM79_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV87671.1"
                     /translation="MLNFKEGHSAYAVVLATLAFLLFFSSIGVKELWRVDETRVAGIS
                     ASMARTGDYWMPRLNGEPFLEYPPMYYWATAGFYQVLGRNPFASRLASALCALGSVFL
                     VFLLAREMGFGPPHSFLAGAILATCGEFWAVANRCLVDMMLCFFIVSAMVCFFKVYRI
                     LHEGETSGGWVLYGFLFSLSLGFAILTKSLVGLAVPSVALFFWLVLECLWNRKPYFKS
                     WLALGFGCLLSAVPALFWLVEIYGNYGMEAVRTVAEVNAFGRFTGSQGDHLAPFFYYF
                     GKVPEQFLPWTVFLAAVPALMLVYRKKPVLDRNARFLVCWILFPFLLLVLSASKRPVY
                     LLPLYPAFALLLSLLLRKATAEIPILARFPWDRIWLSFSALVSLVLVIAGFVLAALYN
                     DKFTFRPLFAYYGKSPCAERPLYLYTPTEAVRGAVNYYLDRDVPVIQDETELEKVLDS
                     RSQPACVIAPHFPGIESRGSVKTFVSKGDPWCVMEGR"
     gene            complement(1869..3914)
                     /locus_tag="PLM79_15035"
     CDS             complement(1869..3914)
                     /locus_tag="PLM79_15035"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOV87672.1"
                     /translation="MKASTIIMFALGCGGLGVLYSLITAAWVSKQPAGSERMQQIAEA
                     IREGATAFLNREYKTVAVVMVVLAAALTYLGKWTAIGFVIGTVGSALAGYIGMMVTVK
                     ANVRTTEAAKGGLQAALTVAFKGGSVTGIMVVGLGLIGITGYYLFVKSVAPVEESFHA
                     LVGLGFGCSLMSVFARIAGGIYTKAADVGADLVGKVEAGIPEDDPRNAAVIADNVGDN
                     VGDCAGMAADLYETYTVTLVAAMLLAKTVFGPESLWVEFPLLIGGISIVASIIGTYFV
                     RLGKSQYIMGALYKGLAVAGILAAGAFYFASDWFLKLPGVEQAFSPMGVFIIAVIGLI
                     LTGLIVMITEYFTSKSFSPVKHIAAASVTGHGTNVIAGLAVSMKAAAPPAIVICGAII
                     WAYSLGGGFAGNPGVGLFAIALTAVAMLSMTGIVVAIDSYGPITDNAGGIAEMAELPE
                     GVRAVTDPLDAVGNTTKAVTKGYAIASAGVAALVLFAEYSRSFAAGLAFDLSDPKVIV
                     GLFIGGLLPYYFGSLLMESVGKAAGGVVEEVRRQFREIPGIMEGTAKPEYGKCVDIVT
                     KSAIKEMMLPALIPVAAPLLVGLLVGKEALGGVLIGAIITGLFQAIAMTSGGGAWDNA
                     KKFIEDGMYGGKKSDAHKAAVTGDTVGDPYKDTAGPAINPMIKVVNIVALLIVPLIK"
     gene            complement(4358..6181)
                     /locus_tag="PLM79_15040"
     CDS             complement(4358..6181)
                     /locus_tag="PLM79_15040"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOV87673.1"
                     /translation="MSQNTIPSPGPIREYVRLREEVLQTLDGMMSIEATSRASLGELR
                     EKIATDTFNLVVVGQFKRGKTCLINALLGAELLPVSVVPLTSIVTILTYGTSLEVKVF
                     TRDGRATFVEPDRIAEYVTEQGNPQNVKNVREVVLTYPSEYLKDGVRLVDTPGVGSVY
                     QHNTDVAYQYLPKSDAALFLLSVDQPVSRAELDFLKDVQQYSGKIFFLLNKIDYLTGK
                     DLEDCLEFSEKSLRDVMGQEVKIFPVSAKLALDGKIADDPELLARSRLTEFSRTLDRF
                     LMNEKGKVLLLSATNSLLRLLAQARLEVELELRSLTIPLEDLKEKVRSFEEKKEEILL
                     ESRAFDILLDGEVNRLVRTLDEELAAFKAEKVPEVLAWFEALCDTNRNAPLQELDLLL
                     ENQIREEVQKVFTPWHARLEDKISAGFEGICRRFLGKMDQVVDDLLAFSSQLFSVPYR
                     PILAEALWRTESGFYFKLRDEPVGLDMLADALTLRVPGMVSRRFEKIKDYLFRKAHER
                     IRRKRKQQLVEAVETQAGRIRYDFLDRLEKSKRKFRVEMRGKIEATMEGIGTAVEKGI
                     TLREKGEREIKDRGKFLERELQELAILEGKVARTREAVSGL"
     gene            6553..7443
                     /locus_tag="PLM79_15045"
     CDS             6553..7443
                     /locus_tag="PLM79_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOV87674.1"
                     /translation="MSALSRRHAAFALILAVGFFLLAFALSAEVSEAAPDPFRPLKER
                     LLKDGFSGPSIARVYQGQPSVQFKTVARTFELRESKLNYDQFLNPSSIEKGRRFASEH
                     RTALRDAGQTYGVDPCVIVAILLVETQFGSYTGKTPTVAVLSTFALMDHKSYRDRIWA
                     MLSPREKARWGREAFDRKLMDRSDWAYREVCALVRLAEDRVRVESLRGSVMGAVGWPQ
                     FLPSSLVRYGADGNRDGRVDLFDPWDACHSVANYLRGYGWCRARTREEKEKVIYQYNH
                     SRPYVQTVLGIADRLQPCES"
     gene            complement(7501..8010)
                     /gene="coaD"
                     /locus_tag="PLM79_15050"
     CDS             complement(7501..8010)
                     /gene="coaD"
                     /locus_tag="PLM79_15050"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOV87675.1"
                     /translation="MEKVAVYPGSFDPITNGHLDLLERGLKIFDRIVIAIAANPAKKP
                     LFSLEERIEMIQQSLANSPFQDRVQVDTFQGLLVEYVRKVRANAILRGLRAISDFEYE
                     FQMALMNRKLSNEIETLFLMTAMRWIYISSRIIKEVVTSGGCVNGLVPEPVELKLVEK
                     LRPALQTSL"
     gene            complement(8051..8605)
                     /gene="rsmD"
                     /locus_tag="PLM79_15055"
     CDS             complement(8051..8605)
                     /gene="rsmD"
                     /locus_tag="PLM79_15055"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOV87676.1"
                     /translation="MRIVAGAFRGRRLLSPRSGRIRPTSDRVREAVFSILAHRVSEAL
                     VLDLYAGTGAMGLEALSRGASRAVFVDDHPEAVRLIRSNIQRCGAEDRARVVRGPVDQ
                     EVRKLALQGEGFHLVFLDPPYGKGLLEESVPRLTPLALPTTLAVAEHRVDDLLPPRLG
                     PWLRIDERRYGDTVVSFFRRECPS"
     gene            complement(8602..>9271)
                     /locus_tag="PLM79_15060"
     CDS             complement(8602..>9271)
                     /locus_tag="PLM79_15060"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOV87677.1"
                     /translation="EHRGIIANPNCSTIQMVVALNPIQKEAGIKRIVVTTFQAVSGTG
                     MRAVAELRSQVHSLSHGQQVTPQVYPHQIAFNCFPHIGAFLENGFSEEEMKMVNETRK
                     IFEDPDIAVCATTVRVPVYHGHSESVNIETRRPISVEEVRRLLSQAPGVRVIDEPSQA
                     RYPVPLEAAGQDDTLVGRIRVDPSAENGLAMWVVADNIRKGAATNAVQIAELLVERNR
                     LSVS"
BASE COUNT         1925 a         2823 c         2737 g         1786 t
ORIGIN      
        1 agcgcccttt tttctactgc ttacaattta cacaaaagat cttacactac cggagggcct
       61 gagatctttc ccccaaggcc gcattccccc agggcatgtg gcacatgaat ccggttttgc
      121 cggtcaccga ccctccatca cgcaccatgg atcgcccttg gagacgaagg ttttgacgct
      181 tccacggctt tcgattcccg ggaaatgagg cgcgatcaca caggccggtt gggaccgcga
      241 gtctagaacc ttctccaact ccgtctcgtc ttggatcacc ggaacatctc ggtcaagata
      301 gtagttcacg gcccctcgaa cggcttcggt gggagtgtag agataaaggg gtctctcggc
      361 gcacggggat ttcccgtaat aggcgaaaag gggtctgaac gtgaacttgt cgttgtagag
      421 ggcggccagg acaaatcctg caatcacaag aaccagggaa acgagggctg aaaaagacag
      481 ccagattcgg tcccatggaa atcgagcgag gatcgggatc tcggccgtcg cctttcgcag
      541 gagcagggaa agcaggagcg caaaggccgg atagagaggc aacaggtaaa ccggcctctt
      601 ggatgccgac agcacgagca gcaggaacgg aaacagaatc cagcaaacga ggaaccgggc
      661 attcctgtca aggaccggct ttttccggta gaccagcatc agagccggga cggcagccag
      721 gaacacggtc cagggaagga actgttccgg cacctttccg aagtagtaga agaagggggc
      781 caggtgatcg ccctgggagc ccgtgaacct cccgaaggcg ttcacctccg ccaccgtccg
      841 caccgcctcc attccgtagt tcccatagat ctcgacaagc cagaaaagcg ccggaaccgc
      901 ggaaagcagg cacccgaatc caagggccag ccaggatttg aaatagggtt tccggttcca
      961 caggcactcg aggaccagcc agaaaaagag ggcaacggaa ggaacggcaa ggcccaccag
     1021 ggatttggtc aggattgcga atcccaggga cagggaaaag aggaacccgt acaggaccca
     1081 acccccggac gtttcgccct cgtggaggat tcggtagact ttgaagaaac aaaccatggc
     1141 ggacacgatg aaaaagcaca gcatcatgtc caccaggcag cggttggcga cggcccagaa
     1201 ctccccgcat gtggccaaga tcgctcccgc caggaaggag tgaggagggc cgaatcccat
     1261 ctcccgtgcc agcaggaaga cgaggaaaac ggacccgagg gcgcagaggg ccgaggcaag
     1321 ccgagatgcg aaagggttcc tgcccagtac ctggtagaac cccgccgtag cccagtaata
     1381 catgggagga tactcgagga agggctcgcc gttcaaccgg ggcatccaat aatccccggt
     1441 ccttgccatg gaagcggaaa tccccgccac ccgggtctca tccactctcc agagctcctt
     1501 cactccgatg gaactgaaaa acaacaggaa ggccagggtc gccagaacga ccgcataggc
     1561 cgagtggccc tccttgaagt tgagcataga cacccctcac ccggacacct gcacaggggt
     1621 ttgaaatggg cccgacctgc cgttcgcgga cgcacaggat ctcatcattc gttcgcttgc
     1681 cttgccatac ggccggtccg gacctcctcg atccaggggc gccacggaca aacgctggtg
     1741 gtccgacggg cgctcgcggc actttagcag tatcctcgag ggtgtcaaac ccaggaaaga
     1801 aaaacccccg accccgggga ggggtggggg gtgattcttt tcttttcggc tccgggccga
     1861 caagcggact acttgatcaa cggcacgatc agcagggcca cgatgttcac gaccttgatc
     1921 atggggttga tggccgggcc ggccgtatcc ttgtacgggt cgcctacggt gtcgcccgtg
     1981 acggccgctt tgtgggcatc ggacttcttg ccgccgtaca tgccgtcttc gatgaacttc
     2041 ttggcgttat cccaggcgcc gccgccgctg gtcatggcaa tggcctggaa aagacccgtg
     2101 atgatggcgc cgatgaggac cccgcccagg gcttcttttc ccaccagcag acccaccaga
     2161 agaggtgcgg ccacggggat cagggccgga agcatcattt ccttgatggc gctcttggtc
     2221 acgatgtcca cgcacttgcc gtactcgggc ttggcggttc cttccatgat tccggggatt
     2281 tcacggaact ggcgccgtac ttcctcgacc acgcctccgg ctgctttgcc cacggattcc
     2341 atgagcagcg acccgaaata gtagggcagg aggcctccga tgaagaggcc gacgatgacc
     2401 ttgggatcgg aaagatcgaa ggccagaccg gcggcaaaag accgggaata ttcggcaaag
     2461 agcaccaggg cggccacacc ggccgaagcg atggcgtatc ccttggtgac ggccttggtg
     2521 gtgttgccca cggcatctag gggatcggtg acggcgcgaa cgccttcggg aagttccgcc
     2581 atttcggcga tcccgcccgc gttgtcggtg atgggaccgt aggagtcaat ggccaccacg
     2641 attcccgtca tggatagcat ggccacggcc gtcagggcga tggcaaaaag gccgacgccg
     2701 ggattgcccg cgaaaccgcc gcccaggctg taggcccaaa tgattgcgcc gcagattacg
     2761 atggcggggg gagcggcggc tttcatgctc accgcaaggc ccgcgatgac gttggtgccg
     2821 tggcccgtga cgctagccgc ggcaatgtgc tttacgggac tgaagctctt ggaagtgaaa
     2881 tactcggtga tcatgacgat caaacccgtc aggatcaggc cgatgacggc gatgatgaac
     2941 acgcccatgg gggagaaggc ctgctccacg ccggggagct tcaggaacca atcggaagcg
     3001 aagtagaagg cgcctgccgc caggatgccg gcaacggcca ggcccttgta gagtgcgccc
     3061 atgatgtatt ggctcttgcc cagccgcacg aagtaggtcc cgatgatgga ggccacgatg
     3121 gagatcccgc cgatgaggag gggaaactcg acccacaggc tttcgggacc gaagaccgtc
     3181 ttggccagaa gcatggcggc aaccagggtc acggtgtagg tttcgtagag gtcggcggcc
     3241 atgcctgcac agtctcccac gttgtcccct acgttgtcgg cgatgacggc ggcgtttctg
     3301 ggatcatcct cgggaatccc ggcttccacc ttcccgacca ggtccgctcc gacgtcggcg
     3361 gccttggtgt aaatgccgcc cgcgatacgg gcgaagacgc tcatgaggga acagccgaat
     3421 cccaggccca ccagggcgtg gaaagattct tccaccggag ctacggattt gacgaaaaga
     3481 tagtagccgg tgatgccgat caacccgagg cccaccacca tgatgcccgt cacggaaccg
     3541 cctttgaagg cgacggtcag ggccgcctgc aggccgccct tggcggcttc ggtggtgcgc
     3601 acgttggcct tcaccgtgac catcatgccg atgtagccgg ccagtgccga gccgacggta
     3661 ccgatgacga agccgatggc cgtccacttg cccaggtacg tcagggctgc ggcgaggacc
     3721 accatgacga cggcgacggt cttgtactcg cgattcaaga aggcggtggc gccttcacgg
     3781 atggcctccg cgatctgctg catcctctcg gacccggccg gttgcttgga cacccaggct
     3841 gccgtgatga gggagtacaa cactcccagc ccaccgcatc caagggcaaa catgatgatt
     3901 gtgctggctt tcatttactc ctcctttgaa gtttttcgaa tcagaacctc cgaagacttg
     3961 tccttgggtc ggataccctc catgaattct gatgccctct tcccgttgac tcgcgtcacc
     4021 tcctccttgt gcgtaaattg aacgttaccg gcgcaaccga aatatacggg gctccattta
     4081 gggctttggc aacccgtgga gcttttcaca cgcatggaaa caggcaaagg gatatggagc
     4141 ggagaatcgg aaactcggcg cggcctttat aaaaaagcga acggtggggt cccttgttcg
     4201 ccttgaatcg cagccttatt gcccgtttgt gagcaaatcc actcaaaata gtcacacaag
     4261 agcctgtaac tagatacttt tttcacatgc ggcacaaacc tactttagca tcctgggttt
     4321 gtcaagcaga aaagcggaaa atgtcaaggg gttgacgtca cagcccggag accgcctcac
     4381 gggtacgggc cacctttcct tccaggatcg caagctcttg aagctccctt tccaggaatt
     4441 tcccccggtc ctttatttct ctctctccct tttctcggag ggtgatgcct ttctcaacgg
     4501 ccgtcccgat tccttccatg gtggcctcga tcttcccgcg catctccacc cggaacttcc
     4561 gcttgctctt ctccaaccga tccaggaagt cgtaccgtat tcttcccgcc tgggtctcca
     4621 cggcctccac aagctgctgc ttccgcttcc gacgaatccg ttcatgggcc ttacggaaca
     4681 ggtaatcctt gatcttctcg aaacgacggc tcaccattcc cggcacacgc agggtgaggg
     4741 cgtcggccag catgtccagc cccacgggct cgtccctcag cttgaaatag aagccggact
     4801 ccgttctcca cagggcctcc gccaggatgg gccgataggg aacggaaaag agctgggaag
     4861 aaaaagcgag caggtcgtcc accacctggt ccatttttcc caggaaccgc cgacagatcc
     4921 cctcgaaacc cgccgagatc ttgtcttcga gccgggcgtg ccagggggtg aaaacctttt
     4981 gcacctcctc gcgaatttgg ttctcgagga gcaggtccag ttcctgtaag ggcgcgttcc
     5041 tgttggtatc acagagggcc tcgaaccagg cgaggacctc cgggaccttc tccgccttga
     5101 atgcggccag ttcctcatcc agagtccgga ccaaccggtt cacttccccg tccaggagga
     5161 tgtcaaaagc ccggctctcc aggagaattt cttccttctt ttcctcgaag gaccggacct
     5221 tttccttgag atcttccagg gggatcgtga gggacctgag ctcgagttcg acctccaggc
     5281 gcgcctgggc caggagccgc agcagcgagt tggtggcgga aagaaggagg acctttccct
     5341 tttcgttcat gagaaaccgg tccagggtcc gggaaaactc cgtcaagcgg ctccgagcca
     5401 gcagctcggg gtcatcggcg atcttcccgt ccagagccag cttggcggat acggggaaga
     5461 ttttcacctc ctgccccatg acgtcccgca ggcttttttc cgaaaactcc aggcaatcct
     5521 ccagatcctt cccggtgagg tagtcgattt tgttgagcag aaaaaagatc ttgccggaat
     5581 actgctgaac gtccttgaga aagtccagct cggcccggct cacgggctga tccacggaca
     5641 gaagaaacag ggccgcatcg gacttgggaa ggtactggta ggccacgtcg gtgttgtgtt
     5701 gatagacgga gccgactccg ggggtatcca ccaggcgtac cccgtccttg aggtattcgg
     5761 acggataggt caggaccact tcccgaacat tcttgacgtt ctggggattc ccctgttccg
     5821 tcacgtattc ggcgatcctg tcgggttcca cgaaagttgc cctgccgtcc cgggtgaaga
     5881 ccttgacctc caaggaagtc ccgtacgtga ggatggtgac gatggaagtg agggggacga
     5941 ctgaaacggg cagaagctcg gctccgagga gtgcgttgat gaggcaggtt ttcccccttt
     6001 tgaactgtcc caccaccacc aggttgaagg tatcggtggc gatcttttcc cgaagctccc
     6061 ccagggaagc ccgagaggtc gcctcgatgc tcatcatccc atccaaggtc tgcaggactt
     6121 cctctcgcag ccgcacatac tcccggatcg gccccggact cggtattgtg ttctgagaca
     6181 tccgtccctg ttcctttaca ccgcacccca aacagaagcc ttccgccacc ctgcccggca
     6241 ctcgcacccc ggcaaacccg gacctcgttg cggaccgttt ccgggatacg attccacggt
     6301 cccgggcgga aaggagcgaa ctcgtttccc gaccctacgg cgaatgcccg accgccaaac
     6361 gctgtccaaa cgagaatcct gccccgggct tcttagcggg caagggcacc aggaaacgag
     6421 gaagtgttgc cggaggagtc ttgccgtttc ttgcggagaa ttcgatgtgt acgtataatg
     6481 gaggctcccg taaagcgcaa cagaaaacgt tttctcgtta ccgcggccct tgaacatgaa
     6541 aaaggaggtc ccttgagcgc gttatcccgt aggcatgcgg catttgccct gatcctcgcg
     6601 gtcggcttct ttcttcttgc ctttgccctg tcggcggaag tttccgaagc cgcacccgat
     6661 cccttccggc ccctaaagga gcgactcttg aaagacgggt tttccggccc ttccatcgcc
     6721 cgggtgtacc aggggcagcc ctcggttcag ttcaaaacgg tcgcccgcac gttcgaactg
     6781 cgggaaagca agctcaacta cgaccagttt ctgaatcctt cgtccatcga aaagggaagg
     6841 cgattcgcat ccgaacatcg aaccgctctc agggacgcgg gacagaccta cggggtcgac
     6901 ccgtgcgtca ttgtcgcaat cctgctggtg gaaactcagt tcgggagtta cacgggcaag
     6961 acccccaccg tggcggtgct ttccaccttt gcgctcatgg accacaagtc ctatcgcgac
     7021 cggatttggg ccatgctgtc ccctcgggaa aaggcacgat ggggtcgtga ggcctttgac
     7081 cgcaagctca tggaccgttc cgactgggct taccgcgagg tgtgcgccct ggtccgtctg
     7141 gccgaggatc gtgtccgggt ggagtccctg cgggggtccg tcatgggggc ggtgggatgg
     7201 ccccagttcc tgccgtcgag cctggtgcgc tatggcgcgg acgggaaccg agatggtcgg
     7261 gtggatcttt tcgacccttg ggacgcctgc cacagcgtgg ccaactacct gcgcgggtac
     7321 ggctggtgcc gggctcgaac ccgggaagaa aaggagaagg tcatctacca gtacaatcac
     7381 agccgcccgt acgtgcaaac cgtcctgggc attgcggacc gactgcagcc ctgtgagtcc
     7441 tgacccttcc gggcggtggg acggctgaga cccctttcct ccccgcagcg ccccccgggc
     7501 ttacagggaa gtctggaggg cgggcctcag tttttccacc agcttcagtt ccacgggttc
     7561 gggaaccaac ccgttgacac aaccgccgga ggtgaccact tccttgatga ttcgggagct
     7621 gatgtaaatc cagcgcatgg ccgtcatgag aaacagggtc tcgatttcgt tgctcagctt
     7681 ccggttcatc agggccatct ggaactcata ctcgaaatcg ctgatggccc gaagccccct
     7741 caagatggcg ttggctctca ccttgcgcac gtattccacc aggagtccct gaaaggtgtc
     7801 cacttggacc cgatcttgga acggggaatt cgccagggat tgttggatca tttcgatccg
     7861 ctcttcgagg ctgaagagcg gtttcttggc agggttcgcg gcgatggcga tcacgatgcg
     7921 atcaaagatt ttgaggcctc tctcgagcag gtccaggtga ccgttggtga tgggatcaaa
     7981 agaacccgga tagaccgcca ccttctccat ctttccctcc ttcgtcacag tcgattcaac
     8041 cgacacaacc tcaacttgga cattcccgcc tgaaaaaaga aaccaccgta tccccgtaac
     8101 gacgctcgtc gatcctgagc catgggccca aacgcggggg caaaaggtcg tccacccggt
     8161 gttccgctac ggccagggtc gtaggaagcg ccaacggggt cagccggggg acggattcct
     8221 ccagcagccc ctttccgtag ggaggatcca ggaacaccag gtgaaatcct tccccttgaa
     8281 gcgccagttt gcgaacctct tgatcgaccg gtccccggac gaccctggcc cgatcctcag
     8341 cgccgcatcg ttggatgttg gatcgaatca gccgaacggc ctcgggatga tcgtccacga
     8401 agaccgcccg ggaagccccc cgactcagag cctcgaggcc catggccccc gtccctgcgt
     8461 agaggtcgag cacgagagct tccgaaaccc ggtgggccag gatgctgaaa accgcttccc
     8521 gaacccggtc gctggtaggt cgaatgcgac cgctccgtgg actgagaagc cgcctgcctc
     8581 gaaaagctcc cgccacaatt ctcatgaaac cgaaagacga tttcgctcca ccaggagctc
     8641 ggcgatctgg acggcgttgg tcgcggcacc cttgcggatg ttgtccgcaa ccacccacat
     8701 ggccagcccg ttttcggcgg agggatccac gcggattcgt cccaccaagg tatcgtcctg
     8761 gccggccgct tccaggggga cgggataacg ggcctgggaa ggctcgtcga tcacccgaac
     8821 tcccggagcc tgggagagca accgccgcac ttcctccacg gagatgggcc gccgggtctc
     8881 gatgttcacc gattccgagt gtccgtgata gaccgggacg cgtaccgtgg tggcgcatac
     8941 ggcgatatcc gggtcctcga aaatctttcg ggtttcgttc accatcttca tctcttcctc
     9001 gctgaagccg ttctcgagga aggctccgat gtgaggaaag cagttgaagg cgatctggtg
     9061 gggatagacc tgcggtgtga cttgttgacc atgggacagg gagtgaacct gggaccgcag
     9121 ttccgcgacg gcacgcatgc cggttcccga cacggcctgg aaggtggtca ccacgatccg
     9181 tttgatcccc gcttccttct ggatgggatt caaggcgacg accatctgaa tggtggagca
     9241 attggggttg gcaatgatgc ctcgatgttc c
//
LOCUS       DAOOVC010000152         9018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4801_length_9018_cov_6.682584, whole genome shotgun sequence.
ACCESSION   DAOOVC010000152 DAOOVC010000000
VERSION     DAOOVC010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4801_length_9018_cov_6.682584"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..447)
                     /locus_tag="PLM79_15065"
     CDS             complement(16..447)
                     /locus_tag="PLM79_15065"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOV87678.1"
                     /translation="MQKENSKELIGIVAVSHCRMAEEMIRVAELIAGRMEACKALSFL
                     PDQPVEEMIRQLRDAIKEVDSGRGVLILTDLFGGTPTNLSLSFLGPKVEVVCGMNLPM
                     LIKLAGCREDTPLREAAAMAKEYGRRHISLASEVLSRSVKG"
     gene            complement(458..1330)
                     /gene="rapZ"
                     /locus_tag="PLM79_15070"
     CDS             complement(458..1330)
                     /gene="rapZ"
                     /locus_tag="PLM79_15070"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOV87679.1"
                     /translation="MERKVHIVVVTGLSGSGKSTAIKAFEDLGYFCIDNLPVPLLPGF
                     LSLCERDMPDVRKIALGIDIRERTFLKDYPEIFSSLEEAGYRFEILFLEAPTDTLQRR
                     YSQTRRVHPAATTESLLVDAIGHERAQLQALRNRADRILDTGSTSVHQLKAQLTRTYG
                     LVNEGERLSVQVLSFGFKYGLPFEADLVVDVRFLPNPYFVEELRSLNGCSPEVSSWVL
                     QWATARDFVADYTGLLLKLLPQYIGEGKRYLTVAVGCTGGKHRSVVIAEEIAGALKEK
                     QYFVNVFHRDMYLE"
     gene            complement(1586..2047)
                     /locus_tag="PLM79_15075"
     CDS             complement(1586..2047)
                     /locus_tag="PLM79_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOV87680.1"
                     /translation="MKILDILPKQAIIPELRGSTKKQVLEELIDALVRHKPQLDRDRL
                     MGVLLERERLGSTGIGDGIAIPHGKLKDLDQLTLSFGRSTRGVDFESMDGKPVHLFFL
                     LVAPETSAGIHLRALAKIARLLKNGQVRKRLGTVAGPDEIYAVIRQEDEDF"
     gene            complement(2143..2679)
                     /gene="raiA"
                     /locus_tag="PLM79_15080"
     CDS             complement(2143..2679)
                     /gene="raiA"
                     /locus_tag="PLM79_15080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOV87681.1"
                     /translation="MQISVTFRQIEPSLPLREYAEEKISRVKKHLEEPIEAHVVLKVE
                     KFRHIAEVTIDANGQRFNGTEETEDMYSAIDKLSDTIESQVKKSKEKIRRRKPGNSNK
                     EFGAMPALAAGADEDEETRVIRTEQVYAKPMDLDEAVMQLNLSAGEFMVFTNRHTNHI
                     NVLYRRKDGHYGLIETLS"
     gene            complement(2750..4201)
                     /gene="rpoN"
                     /locus_tag="PLM79_15085"
     CDS             complement(2750..4201)
                     /gene="rpoN"
                     /locus_tag="PLM79_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOV87682.1"
                     /translation="MAAALELKQQLKLTQQLIMTPQLQQAIKLLQLSRLELLETIHQE
                     LETNPLLEEIQEENFEAEEVEAEGSPVEEAYSEVEVAEKIREDFDWESYLEEYNTSTP
                     VLVETDPNQEWPSYDHRLTAPSTLDDYLGWQLRLSDMTDEEKLIGMLIIGNLNVDGYL
                     DAGVDEVAQMAQTTKDRVEEVLAKVQLFDPLGVGARDLRECLLIQARALHPDNDLVAA
                     ILEHHLQYLEVKNYQGLVRVLKSNPEDVKKAIDVILSLNPRPGRAFNEESVEYISPDI
                     YVLNVDGELVILLNEDGMPKLRVSAYYKEAMSHDAVVPADAKDYIQNKLRSAAWLIKS
                     IHQRQRTLYKVAQSIVKLQKEFFEKGIAYLKPMVLRDVAEDVGMHESTISRVTASKYM
                     HTTHGIFELKYFFNSSINSVLGEAVASESVKERIRQLVRDEDASKPYSDQDIVEILER
                     ENIRIARRTVAKYREVLGILPSSRRKKSFWSGK"
     gene            complement(4214..4948)
                     /gene="lptB"
                     /locus_tag="PLM79_15090"
     CDS             complement(4214..4948)
                     /gene="lptB"
                     /locus_tag="PLM79_15090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOV87683.1"
                     /translation="MHAEKHLAVQGLTKSYQKRRVVDEVDLEVRRGQIVGLLGPNGAG
                     KSTTFYMIVGIIRPDKGRVLLNGENILSMPMYMRARKGVTYLPQEPSVFRKLTVEENI
                     LAILETLDLSRKERSERLRDLLDDLKIAHLAKHRADRLSGGERRRVEITRALVTHPDF
                     ILLDEPFAGIDPIAVMEIQGLVRSLKDKGIGVLISDHNVRETLKVCDWAYIMHEGKVL
                     EAGDPVSIAGSDLARRTYLGDQFSLS"
     gene            complement(4958..5539)
                     /gene="lptA"
                     /locus_tag="PLM79_15095"
     CDS             complement(4958..5539)
                     /gene="lptA"
                     /locus_tag="PLM79_15095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOV87684.1"
                     /translation="MTLRSKFFPGRFWTLMVCVAGCWWFPASAGWTAENQAVPSTPKV
                     LKSESPLHIASDRMEVSQKDRTIQFIGHVTVRQDDLVITGDQMIVYGVQEKGKSKAKG
                     AADTPMVERIDRIEVQGGVRITQREKMATSDKAVYYHREDKIVLLGQPKVSQGQDTLQ
                     GRMITLYLAEGRSVVEGGETAPVQAVLHPSRKE"
     gene            complement(5536..6135)
                     /gene="lptC"
                     /locus_tag="PLM79_15100"
     CDS             complement(5536..6135)
                     /gene="lptC"
                     /locus_tag="PLM79_15100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOV87685.1"
                     /translation="MTLVRKMTQSVILLVALGLSAVLLFGVWKGKTQKDHRIVPVSDA
                     SEAEMRLSDMEYTEMQDGKRLWTLKASEAKYFQDQQRTLLTAVHLTLFLEGGDEIRLE
                     SREGVLYAGTKNIELWNSVHAVLPRSYELITDRAFYEHEQQAVYSETPIRLTGPDVEL
                     EGVSWEYRIPQNRAIVSNGVRATLLLEPPKPSTKAKRTP"
     gene            complement(6384..6941)
                     /locus_tag="PLM79_15105"
     CDS             complement(6384..6941)
                     /locus_tag="PLM79_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOV87686.1"
                     /translation="MGPFDLHGDEPIRARILPLRMMIFDVDGVLTDGRIIYLDDGREL
                     KAFDVQDGHGIKLLQRAGIEVALISGRFSGAVEHRARGLGITRVYQDAKVKLEVYERL
                     LSETDLRDEEVGFMGDDLIDIPVMRRVGFSVAVAGAVSHVFPYAHYVTKAAGGRGACR
                     EVCELILQVQNRWESVTQRYFGPLS"
     gene            complement(7260..8075)
                     /gene="kdsA"
                     /locus_tag="PLM79_15110"
     CDS             complement(7260..8075)
                     /gene="kdsA"
                     /locus_tag="PLM79_15110"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOV87687.1"
                     /translation="MSAFDLLGQSIGNEKLFLIGGPCVIESESMTLKIARSLRESCLK
                     LGVPCIFKASYDKANRTSVQSYRGPGLTQGLQVLARVREDTGLPVLTDVHSVEEAQRA
                     AEVVDVLQIPAFLARQTDLLLAAGKTGKPVNVKKAQFLAPWDMRSVIEKVLHTGNRRI
                     LITERGTAFGYNNLVVDMRSISLLSQWEFPVVMDATHSVQLPGGQGNCSGGERRFVAP
                     LAQAAVAAGAHGVFLELHESPDQALCDGPNSLPLTELSPLLERLSKIYRAVRS"
     gene            complement(8205..>9018)
                     /locus_tag="PLM79_15115"
     CDS             complement(8205..>9018)
                     /locus_tag="PLM79_15115"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOV87688.1"
                     /translation="HEVRIAIVGKYIDLKESYKSLNEALLHGAAAHSLRVELDYVDSE
                     NVEKEGGEALLEGAEGILVPGGFGQRGVEGKIKAIGYARQNKIPFLGICLGMQLATIE
                     FARNVAGLEKAHSMEFDHHTPHPVIFLMREWFDYKNNRVIRRDEDSDFGGTMRLGAYP
                     CLLESGSVAHEAYGVSEVSERHRHRYEFNNEFREVLGKAGMRFTGLSPDRNLVEIIEL
                     PDHPWFLGCQFHPEFKSRPMEPHPIFRAFVGSAMEYARGKRPAEFGKEGLCS"
BASE COUNT         1717 a         2741 c         2570 g         1990 t
ORIGIN      
        1 cgccgcgtcg tccttctacc ctttgaccga gcgggacagg acttcgctgg ccagagagat
       61 gtggcgccgg ccgtattcct tggccatggc cgcggcttcc cgcaaggggg tgtcctcgcg
      121 acaaccggcc agtttgatca gcatgggcag gttcatgccg cagaccacct cgaccttggg
      181 tcccaggaag gacaagctga ggttggtggg ggtccctccg aagaggtccg tgagaatcaa
      241 aacccctctg ccggagtcca cttccttgat ggcgtctcga agctgccgga tcatttcctc
      301 caccggctga tcgggcaaaa aggagagggc cttgcaggct tccatgcgcc cggcgatcag
      361 ctcggccact cgaatcattt cttcggccat gcggcaatgg gataccgcca caattcctat
      421 gagctctttc gagttctctt tctgcattcc atttctctta ttccaggtac atgtcgcgat
      481 ggaagacgtt cacgaagtac tgtttctcct tgagggcgcc cgcaatctcc tcggcaatga
      541 ccacggatcg atgcttcccc ccggtgcatc cgacggcgac ggtcaggtag cgttttcctt
      601 ccccgatgta ctggggaagg agcttcagga gaaggccggt gtaatccgcg acgaaatccc
      661 gggccgtggc ccactggagg acccaggaac tcacctcggg ggaacagccg ttgagactgc
      721 gcagctcctc cacaaaataa gggttgggaa ggaaccgcac atccacgacc aggtcggcct
      781 cgaaaggaag cccgtatttg aagccgaagg agagcacctg gacgctgagc ctttcccctt
      841 cattcaccag cccgtaggtt cgggtgagtt gggctttcag ctggtggacc gaggtgctgc
      901 cggtatccag gattcgatcg gcacggttcc gaagggcctg cagctgggcg cgttcatgtc
      961 cgattgcgtc caccaggagg gattcggtcg ttgccgccgg atgtacccgg cgagtctggc
     1021 tgtagcgcct ctggagggtg tccgtggggg cttccaggaa gaggatctcg aagcggtagc
     1081 ccgcttcctc gaggctgctg aagatctcgg ggtaatcctt cagaaaggtg cgctcccgga
     1141 tatcgatccc cagggcaatc tttcggacat ccggcatgtc ccgttcgcag agggacaaga
     1201 accccggaag cagcgggacg ggcaggttgt cgatacaaaa gtagcccaaa tcctcgaagg
     1261 ccttgatggc ggtactcttt cccgatcccg acaagccggt caccaccacg atatggactt
     1321 ttctctccat gttgtcaaaa atcccgccga cctgattcgg ctcgatgcgg attacgcgtg
     1381 aatgggaaga agcaacgttt ccaggggcct gttgggcaag aaaccgaacc aaaggaaatt
     1441 gcggggctgg aacctcctcc ccctcctgcc tccgggggag tcgggcgcat cgcggaatcg
     1501 caccttccgg tgctcggccg acacgagaat tcggagcacg ggagcgactg ggattcaatc
     1561 ccccctggta ctgcggcttc tccgcctaaa agtcttcatc ctcctgtcgg atgacggcat
     1621 aaatctcatc cggaccggcc acagtcccca gtctctttcg tacttgtccg tttttcagca
     1681 gtcttgctat cttggccaaa gctctcaaat gaattccagc ggaggtttcg ggggcaacga
     1741 ggagaaagaa gaggtgcacg ggtttgccgt ccatggattc gaagtccacc cctcgagtgg
     1801 accttccgaa ggaaagggtg agctggtcca ggtccttgag cttcccgtgg ggaatggcaa
     1861 tgccgtctcc aatccccgtg cttcccaatc gttcccgttc gagcaacacc cccatgagcc
     1921 tgtctcggtc caattggggc ttgtgtcgca ccaaggcgtc gatgagttct tccagaacct
     1981 gcttcttcgt gcttccgcgg agctcgggaa tgatcgcttg tttgggtagg atgtccagaa
     2041 tcttcatcaa tgctgtttgc ccttttgaac gggttgaccg ggaatgcccg gcccaggtcc
     2101 cggtgccccg ggcctggtcg ggtctgcatt cccgtcggaa ttctaactca gggtttcaat
     2161 caatccatag tgaccgtctt tgcggcggta caatacgttg atgtggttgg tatggcggtt
     2221 ggtgaagacc atgaattcac ccgcggaaag gtttaactgc atcacggcct catccaggtc
     2281 catgggcttg gcatacacct gctccgtccg aatgacgcgg gtctcttcgt cttcgtcggc
     2341 tcccgccgcc aaggcgggca ttgcgccgaa ttccttgttg gaattgccgg gttttcgccg
     2401 ccggattttc tccttgctct tcttcacctg gctctcgatg gtgtccgaaa gcttgtcgat
     2461 ggccgagtac atatcttccg tttcttcggt accgttgaat cgctggccgt tggcatcgat
     2521 ggtgacctcg gcaatgtggc gaaatttctc gaccttgagc accacatggg cctcgatggg
     2581 ctcctcgagg tgtttcttga ctcgagagat cttttcctcg gcgtattctc gaaggggcag
     2641 tgacggttca atctgacgga aagtcacact gatttgcatg gatggaaacc tccggcgtaa
     2701 aattaaccca attccaacaa cggggaaacc cggtccccat gcggcccttt tatttgccgc
     2761 tccaaaagga tttcttcctt ctgctagaag gcaatatgcc cagcacctca cggtatttcg
     2821 ccaccgtgcg ccgggcaatc cggatgttct ccctttccag gatttctaca atatcctgat
     2881 cgctatatgg cttggacgca tcttcatccc ggacaagctg ccgaatgcgt tccttgacgc
     2941 tttcggacgc cacagcttct cccagaacgc tatttatgga gctgttaaag aaatatttca
     3001 actcaaaaat cccgtgagtc gtatgcatgt atttgctggc ggtgactcga ctgatggtgg
     3061 attcgtgcat gccgacgtct tcggccacat cccgaagaac catgggcttt aggtaggcaa
     3121 tccccttttc gaagaactcc ttttgaagct tcacgatgct ttgggccacc ttgtacaggg
     3181 tccgttgacg ctgatggatg ctcttgatca accaggccgc cgatctcaat ttgttctgga
     3241 tgtaatcctt ggcgtcggca ggcactacgg catcatggct catggcttcc ttgtaataag
     3301 cgctcacccg cagcttcggc atgccgtctt cattcagaag aatcaccaat tccccgtcca
     3361 cgttgaggac atagatgtcc gggctgatgt attcgacgga ctcctcgttg aaagcccgcc
     3421 ccggtcgagg gttcaggctc aggatgacgt caatggcctt cttgacgtcc tcggggttgc
     3481 ttttgagcac ccggacgagg ccctggtagt tcttgacctc gaggtattga agatgatgtt
     3541 cgagaatggc ggccaccagg tcgttgtcgg gatggagcgc ccgggcctgt atcaaaagac
     3601 actccctcaa atcccgcgcc ccgaccccca ggggatcaaa cagctgaacc tttgccagga
     3661 cctcttccac tcggtccttg gtggtttgcg ccatttgagc gacctcgtcc acccccgcgt
     3721 ccaggtaccc gtccacattc aggttcccga taatcagcat cccgatgagt ttctcctcgt
     3781 cggtcatgtc ggagagccgc agttgccagc ccaggtagtc gtccagggtg gaaggggcgg
     3841 tgaggcggtg atcgtaggag ggccattcct ggttgggatc cgtttccaca aggaccggcg
     3901 tgctcgtatt gtactcctcg aggtaggatt cccagtcgaa gtcctcccgg atcttctcgg
     3961 ccacttccac ctcggaataa gcttcctcga cgggcgaacc ctcggcctcg acctcctcgg
     4021 cttcgaaatt ctcttcctgg atctcctcga gaagcggatt cgtctccaac tcctggtgaa
     4081 tggtttccag gagctccagg cgggagagct gcaacagctt gatcgcctgt tggagctgag
     4141 gcgtcatgat cagctgttgg gtgagcttca gttgctgttt cagttccaat gccgctgcca
     4201 ttcctctttc ctgtcaggat agactgaact ggtctcccag gtaggtccgt cgagcaagat
     4261 cgctgccggc gatgctcacc gggtctcccg cctccagcac cttcccttcg tgcatgatgt
     4321 aggcccaatc acacactttg agggtttcac gaacgttatg gtcggaaatc agaacgccga
     4381 ttcccttgtc cttgagagac cgaacaagcc cctggatctc catgactgca atggggtcaa
     4441 tccccgcaaa cggctcatcg agcaggatga agtcgggatg ggtcaccagg gcgcgggtga
     4501 tctccacccg gcggcgctct ccccccgaca gccgatccgc ccggtgcttt gccaggtgag
     4561 cgatcttcag atcgtccagc aaatcccgga gacgctcgct gcgttccttc ctcgaaaggt
     4621 ccagggtctc cagaatcgcc aggatgttct cctccaccgt cagcttgcga aaaaccgagg
     4681 gctcctgggg caaataggtg accccctttc ttgccctcat gtacatgggc atggacagga
     4741 tgttttcccc gttgaggagg acccggccct tgtccggacg aatgatcccc acgatcatgt
     4801 aaaaggtggt ggactttccc gctccgttgg gccccagcag ccccacgatc tgtccccggc
     4861 gaacctccag gtcgacttcg tccaccactc gccgtttctg atagctcttg gtcaaaccct
     4921 ggaccgccaa atgcttctca gcgtgcagcg acaaggttca ctccttgcgg gaaggatgaa
     4981 gaaccgcctg taccggggcc gtttctcccc cttccaccac gctgcgcccc tcggccaggt
     5041 acagggtgat catccgaccc tgaagagtgt cctgtccctg ggataccttc ggctgtccca
     5101 ggaggacgat cttgtcttcc cggtgatagt atacggcctt gtcggaggtg gccatcttct
     5161 ccctctgggt gattctcacg cccccctgca cttcgatccg gtcgatgcgc tccaccatgg
     5221 gagtatccgc cgcacctttc gctttgcttt ttcccttttc ctggaccccg tagacgatca
     5281 tctggtcccc cgtgatcacc agatcgtcct ggcgcacggt gacgtgcccg atgaattgaa
     5341 tggtcctgtc cttctggctc acctccatgc gatcgctggc gatgtgaaga ggagactcgc
     5401 tcttgaggac tttgggagta gagggtacag cctggttctc cgcggtccat cccgcggaag
     5461 ccggaaacca ccagcatccc gcgacacata ccatcagggt ccaaaatcgg ccgggaaaaa
     5521 acttactgcg caatgtcatg gggtcctctt cgctttggtg gaaggtttcg ggggctcgag
     5581 cagcagggtc gcgcgaactc cgttgctcac aatggcccga ttctgcggaa tcctgtactc
     5641 ccagctgact ccctccagtt ccacatcggg ccccgtcagg cggatgggag tctcggaata
     5701 aaccgcctgc tgctcatgct cgtagaaagc ccggtccgtg atcagctcgt aggaccgggg
     5761 aagcacggcg tgaaccgagt tccacaactc gatgttcttc gtcccggcgt aaaggactcc
     5821 ctcgcggctc tccagcctga tttcgtctcc tccctccagg aacaaggtaa ggtgtacggc
     5881 ggtgagaagg gtcctctgct gatcctgaaa gtacttggcc tcggacgcct tcagagtcca
     5941 aagtcgcttg ccgtcctgca tttcggtgta ttccatgtcg gaaagccgca tctcggcttc
     6001 cgaagcgtcg cttaccggaa caatgcggtg atccttctgg gtcttgccct tccagacccc
     6061 gaacaggagc acggcgctca ggcccaaggc caccaggaga atcacgctct gggtcatttt
     6121 gcgcaccaac gtcatgggac tcccttctgc cgggaccgtt gaaacgcccc caaggattca
     6181 ccgggagcga atggtagtga attcaatcgg ttgaccggtc tgcttgggtg aagccatgaa
     6241 gccattccca gtttttccaa tcaaattaac aaattatatt attgcctact gtaacacgag
     6301 cagggagggc tgtaaaggca tattctgtgc caggaagagt cccgtgggcg ggattttcat
     6361 catgggaacc gcaaaaagga gggtcaggag agggggccga agtatcgttg ggtgaccgat
     6421 tcccaccgat tctgcacctg caggatcaat tcgcagacct cccggcaagc cccgcgtccg
     6481 cccgcagcct tggtgacgta gtgggcgtaa gggaagacat gggaaacggc tccggcgact
     6541 gccacggaaa acccgacgcg gcgcatcacg ggaatgtcga tgaggtcgtc gcccataaag
     6601 cccacctcct catcccgcaa gtcggtctcg gacaacagcc tctcatagac ctccagcttg
     6661 accttggcat cctggtacac ccgggtgatg cccagacccc gggctcgatg ttccacggcc
     6721 ccgctgaacc ggccgctgat caaggccacc tcgatccccg ccctctggag cagcttgatg
     6781 ccgtgaccgt cctggacgtc gaaggctttc agctcccgcc cgtcgtccag gtagatgatt
     6841 cggccgtcgg tcagcacgcc gtccacgtcg aaaatcatca tgcgcagggg caggatccga
     6901 gcccggattg gttcatcccc gtggaggtcg aagggtccca tgaagcttcc ttcctagact
     6961 gtgcgatctt gctggtatga gtcccgtttc gtcattccta tgaaagggag actttcattc
     7021 aacggggcgc acccccgtgc atgaaggatt cccgggcagg cggaaatccg tgaaaggcat
     7081 acctggcctt ggatccccgc tgtcgtgggc aggacgggcg gacgctcgcg gattcgctca
     7141 actcaaatcc ttttcggcgg acaatcgggc cgttgccttg catcccggcc tcccggaccc
     7201 aggatttccc gggggatccg ggccgggaga gggtgacccg gcgaaccggg gttggggtcc
     7261 taggatcgta ccgcccggta aatcttcgac aggcgttcca gcaaggggga caactccgtc
     7321 aagggaaggg aattgggccc gtcgcacaag gcctggtcgg gagactcgtg cagttccaga
     7381 aagaccccgt gagctcccgc agccacggcc gcttgggcaa gaggggccac gaaccggcgt
     7441 tctcctcccg agcagttgcc ctgtccgccg ggaagctgaa cgctgtgggt ggcgtccatg
     7501 accacgggaa actcccattg gctgagaagg gagatggaac gcatgtccac caccaggttg
     7561 ttgtacccga aggccgtgcc ccgttcggtg atgaggatcc ggcggtttcc cgtatgaagg
     7621 actttctcga tcaccgatct catatcccag ggagccagga actgggcctt cttgacattg
     7681 acgggctttc cggttttccc ggcggcgagg agcaggtccg tctgcctggc cagaaacgcc
     7741 ggaatctgca gaacgtccac cacttccgcc gcccgctgcg cctcctccac gctgtggaca
     7801 tccgtgagga ccggcaggcc cgtgtcctcc cgcacgcggg caaggacctg cagtccctgg
     7861 gtcaacccgg gtccccgata ggactggacg gaagtacgat tggccttgtc gtaggatgcc
     7921 ttgaaaatac agggtacccc cagcttcagg caggattccc ggagggatcg cgcgattttg
     7981 agggtcatgc tttcggattc gatgacgcag ggccccccga tgagaaacag cttttcgttt
     8041 ccgatggatt gtcccaacaa atcaaaggcc gacacggtca gactcccttc cgggacatca
     8101 gctcgacccc ctgtgcaaaa gcctttcggc cttttgggcg agggggacgt ttggaggaaa
     8161 caagcgccgg gcgcccggga atcgcccctc gatgggggtg ggggtcacga acacagccct
     8221 tccttgccga actcggcggg ccttttcccc cgggcatatt ccatggcgct tcccacgaaa
     8281 gcccggaaga tcgggtgggg ttccatgggc cgggatttga actccggatg aaactggcac
     8341 cccagaaacc acggatgatc gggcagttcg atgatttcca ccaggttgcg atccggcgaa
     8401 agccccgtaa atctcattcc cgccttgccc aggacctccc gaaattcgtt gttgaactcg
     8461 tatcggtggc gatgccgctc ggaaacttcg gaaaccccgt aggcctcatg ggcaacactg
     8521 ccggactcga gcaggcaggg gtaggccccc aaacgcatgg tgcccccgaa gtccgaatcc
     8581 tcgtctcgtc ggatcacccg gttgttcttg tagtcgaacc attccctcat gaggaaaatg
     8641 acggggtgcg gcgtgtgatg gtcgaattcc atgctgtggg ctttttccag gcccgcgacg
     8701 ttgcgggcga actcgatggt ggccagttgc atgcccaggc aaatgcccag gaaggggatt
     8761 ttgttctgcc gggcataccc gatggctttg atttttcctt ccacgccccg ctgtccaaac
     8821 cctccgggca ccaggatgcc ctcggcgcct tccaggaggg cttccccccc ctccttctcc
     8881 acgttctcgg aatccacgta atccaactcc acgcgcagac tgtgagcggc cgccccgtgg
     8941 agcagcgctt cgtttaggct cttgtaggat tccttgaggt cgatgtattt gccgacgatg
     9001 gcgatgcgga cctcgtgg
//
LOCUS       DAOOVC010000153         9001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4810_length_9001_cov_5.642186, whole genome shotgun sequence.
ACCESSION   DAOOVC010000153 DAOOVC010000000
VERSION     DAOOVC010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4810_length_9001_cov_5.642186"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PLM79_15120"
     CDS             complement(<1..1191)
                     /locus_tag="PLM79_15120"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOV87689.1"
                     /translation="MALRGEVGPHFRDRSWAYAYKTSTLREDGSLVDILHEFYIPVLE
                     RSKAYDRVAGYFTSSSLAAASQGYSAFTAHGGRMRLIVGADMDPQDVAAVLSGEEARL
                     EKHLDSELEGTWPEDVTRGIELLAWMVSQGFLELRVAFRLHGKTGEPLPFESQEDGYV
                     HEKWALFTDKAGHRILASGSLNESKTSLVQNAENLIIDCDWWEGPSADRIRDHQKDFD
                     ALWENRHPHFRVLTLPEAVHRKLVRIGERVRIPREVDGTPRKIPPKPTPEEWLAFKVI
                     QMAPKMPGGLWVGMETAPVSPWPHQEVVARRLIENWPSSFMLCDEVGLGKTIEAGLAL
                     RSLILSGVVKRVLVAPPAGLAQQWHREMASKFYLPFRRGVASPVKKHEVLFPDQKWKP
                     ADNLF"
     gene            complement(1194..4445)
                     /locus_tag="PLM79_15125"
     CDS             complement(1194..4445)
                     /locus_tag="PLM79_15125"
                     /inference="COORDINATES: protein motif:HMM:NF018357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOV87690.1"
                     /translation="MTEKENGRRLIEAGFPCHQVGAETQRERGSSSALPPLYYLHVWW
                     ARRPLTPSRAAVLGSILPAGTNPDWFLKELGIEKKVLHMEGGEWVLVGKNKDLIRVND
                     GEAETLIFDTAAAAAFGKENERRTECRKTIETLKKSDPDLAKDPVLLRWEKETQPLKR
                     PTHKAILDVSTIAADPAHIKDRIAFAKSSPVTKALHQEIRWDPEDLYGYERAFTTPPK
                     TSYQDVSVMDITAGGGSIPFEALRLGCRTLANELNPVASVILEATLNYPATYGKDLLK
                     DIERWGNRLIESVARKMDPVTPFSSLPPEEIQVLHAHCKKDPSVIKDFSGPEYDQTGL
                     LYSRQVTCPHCGGEAPLLNTCWLSKADGEPWGVAVLPDGKKKNGTVRFKTYRVTKGKG
                     PNGEDPNYATVSGGKGLCVHCGQGIDEEEIKRQARGESEHGKWRDRLYAVAAVRYQPK
                     LDKNGQIQRYKTGEKAGEIKTEKVRFFRPPNERDFAALDEAERMLKENWNRWEDAGLI
                     PTESFPQGNDMRPVIYGMTRWCDLFTPRQLLGHLFLIEGLNHLKPQILKELGHEKGKA
                     VVTYLQFAIDKGLDYNSRQTRWHYSRGVMINTFGRHDYSLKWTYGEMVFTGPNSGAAW
                     GLSQIMDAYKGIAELLEPVKEKTGGKPPLTLINGTAAAMPQVPDRSVDVVCFDPPYYN
                     NVQYAELSDYFYVWQKRTLKDLYPELFHRRLTNKEDEAVANPDRDGGEKGAKAAYERM
                     MGEIFLEARRILKDDGLMTMMFTHKSQEAWETLTRALIEKGWVITSSMPVESETEQGI
                     HTKDTASAISSIFISCRKAIRGENQPPSSWKGFGSSGVQQKVQTHVEKGLEELAPLKL
                     NPVDRMVASYGRALQVLSENWPVLDGDEPVGPKRAMLEASRVVAEAQFREIAQGQLAL
                     SDLSPEAAMAMLLFGIFGLGDFGYDDALNLSKSLNIALETKTGGYAANGRFIGINTQE
                     SGRRAAGLNAHESGFAAPLVRKGSKLRLALPEERDPRRLATPQTEWDLLQGTIMAYRE
                     GMEISARGYIQNKAGEKAPIVKSLLNVWAEEVAEEALKKEARAILFGLGLSEA"
     gene            complement(4465..5073)
                     /locus_tag="PLM79_15130"
     CDS             complement(4465..5073)
                     /locus_tag="PLM79_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87691.1"
                     /translation="MEKIVRSSGLDLDQHFDTYLKPALKVAGWTLRVTDHSDKPSPVL
                     IVKEHIRIPGEKTPSGKDKTTILDRGALFGDALVRLLPLVRKILSGIRNEQGIPLEAQ
                     RYIEGLPVAYRGNLPLDEEAGFKFALMFKLQERLKELDRVELMARRVEKFSREEAAYW
                     YSRISDFSIEANRWAQAGLRIMLAGQPHDPAIQTMLEKLRRN"
     gene            complement(5078..8308)
                     /locus_tag="PLM79_15135"
     CDS             complement(5078..8308)
                     /locus_tag="PLM79_15135"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOV87692.1"
                     /translation="MGVLKSCQPRKDLLSGSINMEIFTASLSQVANFYKGQKSGHPLY
                     TDAATFFSEGTHFTEDMKMILRDVVFRLGGDTTASALKRLETGFGGGKTHTLIACLHL
                     AKKGKDLAPYVAEFIEKEILPDPGTVDIVAIAGDAVPVKESRGTELKPYPLWTEIARQ
                     LGGTELCSEVTSYLDRLDSPDEAFFESLFAGRKVLILIDELAHYAARWAVAYPRAKTM
                     LATFFMSLLGYASQHPGIAIVVTLAGLHDAFSAQTAELSKTLSDLSSQPVDPDQALTL
                     QQDAARELTTVLARTETVVVPVKAKDLSAVLGKRLFEQIDREEARRTVEEYRQLYERN
                     PSLLPGSLQMEPFLEEMRAAYPFHPALLDLLNKKLSTVPTFQKTRGVLRILALAVRSL
                     WKSGNDVPMIHPGHLDFMDSRTVDELLGRTNNTSMIPVLNADIGTTDTTGLQGGRSNA
                     ERADQENPHPDKIPYHVQTWKTIFLNSLAGQAEGLASPVFGINESDALLSVAQPGLTP
                     AQAKQALEDIKTKAFYLREKNGLYYAVSEPNVNLALSRIRDAVTEEQILTELESKARN
                     LFGKNRQGFLVKPDVIGPQDLPDKLERPLLGVLSIRLTEVDPAAFITTVGKGIPRLYQ
                     NSVFLFIPETVRILRSGEEGRLIPEGEEQRERAKLELYALARNVLAAKILKDTPQNFG
                     ISASQVKEIDFPRLDNDLERRVSLVYRFLAYPVKGGIFALKEIRTAGGEGGDAQFEQI
                     RTVLKENGEIIPSGPVTKEILLGLKTLFFEEKDYISLDRIREKFHVNRGWPVLEEQEF
                     LSTIAREGVKKGFWCLAGKIDEKTGKPEKFYDAEKSDIPLHEELSPDDVLITIPGAKQ
                     RGWGTQSGPTKEEIRRLVESATEEKKGLRVAQVHEHVKGLRDDAAEEIINETLVDFAR
                     KEQLLAYEGDPEQAEKPAKLVSGIEAIRMTPTPELVVITRGEAVRRGWLAEKPNVLKV
                     SGSEGVTVFSSGLLRKLGSLYTRGAKSCFHRFDVTEFDLPTGGRFSLTLRDVSPEGMK
                     QLGEFFEILGDKLTPSSESELILEIKNPDEACPLVQEIQRLLKGKE"
     gene            complement(8333..8683)
                     /locus_tag="PLM79_15140"
     CDS             complement(8333..8683)
                     /locus_tag="PLM79_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87693.1"
                     /translation="MLAESRPWQPDVKGALFDREKALTNGWVEQGGPTPLVGIPKTRG
                     VWAKEIYPVHRCDFQTIVIFLMIKVNAKNQITKTEARVSTVSMMGKVEKLCTELFALD
                     EERANEELQAVLQK"
BASE COUNT         1762 a         2571 c         2618 g         2050 t
ORIGIN      
        1 gaagaggttg tccgcaggtt tccatttctg gtcggggaag aggacctcgt gtttcttgac
       61 gggactggcg acaccgcgcc gaaagggaag ataaaacttc gaggccattt cccggtgcca
      121 ctgttgggcc aatcccgcgg gtggagcaac gaggaccctt ttcaccacac ccgaaaggat
      181 aagggaacgc agggcaagcc ccgcctcgat ggtctttccc aggcccactt cgtcgcagag
      241 catgaagctc gagggccagt tttcgatgag gcgccgcgcc acaacttcct ggtgcggcca
      301 aggggaaacc ggggctgttt ccatgccgac ccagagccct cccggcatct ttggggccat
      361 ctggatcacc ttgaacgcca gccactcctc gggggtgggt tttgggggaa tcttccgggg
      421 ggtgccatcc acctcacggg ggatccggac ccgctcgccg attcggacca gcttgcggtg
      481 aaccgcttcg ggaagggtca ggacccggaa atgaggatgc cgattttccc acaaggcgtc
      541 aaaatccttc tgatggtccc ttatccggtc ggcagagggt ccctcccacc agtcacagtc
      601 aatgatcagg ttctcggcgt tctggacgag ggacgttttc gattcgttca gggagccgga
      661 agcaagaatt cgatgcccag ccttatccgt gaaaagagcc catttctcat ggacgtagcc
      721 gtcctcctga gattcgaagg gaaggggttc accggttttc ccgtgcagtc gaaaggcgac
      781 acggagttcg aggaatccct ggctcaccat ccacgccaga agctcgattc cccgtgttac
      841 gtcctctggc caggttccct ccagttccga atccaggtgc ttttccaggc gggcttcttc
      901 acccgaaagc acggctgcca catcctgggg atccatgtct gcgcccacga tgagccgcat
      961 gcggccaccg tgagccgtga aagcggaata cccctgagag gccgcggcca aagaggagga
     1021 agtgaagtag cctgcaaccc ggtcgtaagc cttggaacgc tccaggaccg ggatgtagaa
     1081 ctcgtggaga atgtccacga gactcccgtc ctcccgaagc gtcgaggtct tgtaggcgta
     1141 ggcccaggac cggtcacgaa agtgcgggcc aacctcacct cgcaaagcca ttctcaggcc
     1201 tccgaaagac ccagcccgaa caggatggcc cgggcctctt tcttcagagc ctcctcagcc
     1261 acttcttccg cccagacatt gagaaggctt ttcacgatgg gagccttttc accggccttg
     1321 ttctggatat agccgcgggc agagatttcc atcccttccc ggtaggccat gatcgttccc
     1381 tgcaggagat cccattccgt ctggggggtc gccagacgac gaggatcccg ttcttcgggg
     1441 agagccaggc ggagcttgct tcctttgcgg acaagcggag cggcaaaacc ggattcgtgg
     1501 gcgttcagcc ccgccgcccg ccggccgctt tcctgggtgt tgatgccgat aaagcggccg
     1561 ttggcggcat acccaccggt cttggtctcc agggcgatat tcagggattt cgacaggttc
     1621 agagcatcgt catagccgaa atcacccaag ccgaagatgc cgaaaaggag catcgccatg
     1681 gcggcttcgg gagaaaggtc cgaaagggcc agctgtcctt gggcaatctc ccggaattgg
     1741 gcttccgcga cgacacggga ggcctcgagc attgcccgct tcgggccgac gggctcgtct
     1801 ccgtcaagaa cgggccagtt ttccgacaag acctgcaggg cgcggccata gctagccacc
     1861 atccggtcca ccgggttcaa tttcaggggg gcgagttcct cgagcccttt ctcgacatgg
     1921 gtttggacct tctgttgaac accggagctc ccaaagccct tccatgagct ggggggctgg
     1981 ttttctcccc gtattgcctt cctgcaggaa atgaagatgg aacttatggc ggaagcggtg
     2041 tcctttgtat gaattccctg ttcagtttcc gactcgaccg gcatcgatga agtgatgacc
     2101 caccctttct caatgagagc ccgtgtcaac gtttcccagg cttcctggct cttgtgggta
     2161 aacatcatcg tcatgagtcc atcgtccttc aggatccgcc gtgcctccag gaagatttcg
     2221 cccatcatgc gctcgtaggc tgccttggcc cccttctctc ctccgtcacg atccgggtta
     2281 gccacggctt cgtcttcctt gttggtgagg cggcgatgga agagttccgg ataaaggtcc
     2341 ttgagcgtcc gcttctgcca gacgtagaaa tagtcggaaa gctcggcata ctggacgttg
     2401 ttgtaatagg gggggtcgaa gcagacgaca tccaccgatc tgtcaggaac ctgcggcatg
     2461 gcggcagcag tgccgttgat gagagtcagg gggggcttgc cgccggtttt ctctttcact
     2521 ggttccagaa gttccgcgat ccccttatag gcatccatga tttgtgagag tccccaggcg
     2581 gctccagaat tcgggccagt gaagaccatt tctccatagg tccatttaag agagtaatca
     2641 tgtcgcccaa aagtgttgat catgacgcct ctgctataat gccaccttgt ttgccggctg
     2701 ttgtaatcca accccttgtc aatggcaaac tggaggtagg tgaccacggc cttgcccttt
     2761 tcatggccca attccttcag aatttgaggc ttcaggtggt tcaacccctc gatgaggaaa
     2821 aggtgcccca aaagctggcg cggggtgaac aggtcgcacc agcgggtcat gccatagatg
     2881 accggccgca tatcattgcc ttgagggaag ctctccgtgg ggatcaggcc ggcgtcttcc
     2941 cagcggttcc agttctcctt cagcatccgc tccgcctcgt ccaaagcggc gaagtcgcgc
     3001 tcgtttggcg gccggaagaa acggaccttt tccgtcttga tttcccccgc tttttccccg
     3061 gtcttgtacc gctgaatctg accgttcttg tcgagtttcg gctggtatcg cacagccgcc
     3121 acggcataga ggcgatccct ccatttcccg tgctcggatt cacctcgggc ctgccgcttg
     3181 atctcttcct cgtcgatccc ctgtccgcaa tggacgcaaa gccccttgcc gccgctcacc
     3241 gtcgcatagt tgggatcctc cccgttgggg cctttacctt tcgtgacgcg gtaggtcttg
     3301 aaccgaaccg tgccgttctt tttctttcca tctggtagaa cggcaactcc ccagggctct
     3361 ccgtctgcct tggagagcca gcaggtgttg agaagcgggg cctcgccgcc gcaatgggga
     3421 caggtcacct gacgagagta gagaagcccc gtttggtcat attctggccc actgaaatcc
     3481 ttgatgacgg aaggatcctt tttacaatgt gcgtggagta cttgtatctc ttcaggaggg
     3541 agggaagaga acggggtcac ggggtccatc tttcgtgcga ccgattcgat caaccggttc
     3601 ccccaccgtt cgatgtcctt taggagatcc ttgccataag ttgctgggta atttaaggtc
     3661 gcctcgagga tgaccgaggc tacagggttc agttcgttgg ccaaggtcct gcaacccaga
     3721 cggagggctt caaacgggat agatccacct cccgccgtga tgtccatcac ggaaacatcc
     3781 tggtaagaag tcttcggagg ggttgtgaat gcgcgctcgt atccgtagag gtcttctggg
     3841 tcccaccgga tttcctgatg aagggccttc gtcacaggtg aggatttcgc gaaggcaatc
     3901 cggtccttga tatgggccgg gtccgcggcg atagtgctga catccagaat ggctttatga
     3961 gtgggccgct tgagaggttg ggtttccttt tcccaccgca ggaggaccgg gtccttggcc
     4021 aggtcgggat cgctcttctt cagagtttct atggttttac ggcattccgt ccgtcgttcg
     4081 ttctcttttc cgaacgcggc agccgccgcg gtatcgaaga tcagcgtttc ggcttcacca
     4141 tcattaactc ggatgagatc cttgttcttt ccgacaagga cccactctcc gccctccata
     4201 tggagaactt tcttttcgat ccccaattcc ttcaaaaacc agtcggggtt ggttcccgcc
     4261 gggaggatgg agcccagaac cgcggcacgg ctgggtgtca aggggcgacg ggcccaccag
     4321 acatgaaggt agtagagagg gggcagggcc gagctggagc ctcgttccct ctgcgtttcg
     4381 gcccccacct gatggcaggg aaatcccgct tcgataagcc ttcggccgtt ttctttctcc
     4441 gtcatggctt tcttgtcatc tccttcaatt ccggcgcagt ttctcgagca tcgtctgaat
     4501 ggctgggtca tggggctggc cggccagcat gatccggagg ccggcctggg cccagcggtt
     4561 ggcttcgata ctgaaatccg agatccggga ataccagtag gcggcttcct cccgggagaa
     4621 cttctccaca cggcgggcca ttagttccac ccggtccagc tccttcagac gctcctggag
     4681 cttgaacatg agagcgaact tgaaacccgc ctcttcgtcc agggggaggt ttccccggta
     4741 agcgacggga agtccttcga tgtagcgctg ggcctccagg gggatgccct gctcgttgcg
     4801 gatccccgag aggatctttc ggacgagggg caaaaggcgg accagggcat cgccgaacag
     4861 ggccccgcga tccaggatgg tcgttttatc cttgcccgaa ggggtttttt cgccaggaat
     4921 gcggatatgc tccttcacga tgaggactgg gctgggtttg tccgaatggt ccgtcacgcg
     4981 cagggtccac ccggcaactt ttagcgcggg cttcaggtag gtgtcaaaat gctggtctaa
     5041 atccaagccg gaggagcgga cgatcttttc catggggtca ttcctttcct ttcaacaacc
     5101 gctggatctc ctggaccagc gggcaggctt cgtcggggtt cttgatctcc aaaatgagtt
     5161 cgctctccga agaaggcgtc agtttatcac ccaggatctc gaaaaactct cccaactgct
     5221 tcatcccttc cggactcaca tcccgcagcg taagggaaaa acgccctccc gtcgggaggt
     5281 cgaattcggt cacatcgaac cgatggaagc agctcttcgc cccccgggta taaagactac
     5341 ccagttttct caataaaccg gacgagaaga ccgttacccc ttcggaaccg gaaaccttca
     5401 ggacattggg cttttctgcg agccagcccc tccggaccgc ttcaccccgg gtgataacga
     5461 ccaattccgg tgttggcgtc atccggatgg cctcgatgcc ggagaccagt ttcgccggtt
     5521 tctcggcttg ttcggggtct ccctcgtagg cgagaagctg ctctttccgg gcgaagtcca
     5581 ccagggtctc gttgatgatc tcctccgcgg cgtcgtcgcg gaggcccttg acgtgctcgt
     5641 ggacctgggc cacccttagg cccttcttct cctccgtggc cgactccacc aggcggcgga
     5701 tctcctcttt cgtagggccc gactgggttc cccacccccg ctgctttgca cccggaatcg
     5761 tgatcaggac gtcgtccggg ctcaactctt cgtgaagagg gatatccgat ttttcggcgt
     5821 cgtagaattt ttccggtttc ccagtttttt cgtcgatctt tccggccagg caccaaaacc
     5881 ccttcttcac gccctcgcga gcgatggtcg acagaaactc ctgttcttcc aggacgggcc
     5941 agccccggtt cacgtggaac ttctctcgga tacggtccag ggaaatgtaa tctttttctt
     6001 cgaagaagag ggtcttgagc ccaagaagaa tttctttcgt gacgggacca gaggggatga
     6061 tctccccatt ctctttcaaa acggtgcgga tctgctcaaa ctgcgcatcg cctccctcgc
     6121 ctccggccgt tcggatttcc ttgagggcga agatcccacc cttaaccgga taggcgagga
     6181 aacggtagac aaggctcacc cgcctttcca ggtcgttatc cagcctgggg aaatcgattt
     6241 ccttcacctg agaggcagaa atgccgaagt tctggggggt atccttcagg attttggccg
     6301 caaggacatt tcgagcaagg gcgtaaagct cgagcttggc cctttcccgc tgttcctccc
     6361 cttccgggat gagacgcccc tcctcgccgg aacgaaggat gcggaccgtt tccgggatga
     6421 acaggaagac gctgttctgg taaaggcgag gaattccctt gcccacggtg gtgatgaagg
     6481 cggcaggatc cacctcggta agccggatgg agaggactcc cagcagagga cgctccagtt
     6541 tgtcgggaag gtcctgagga ccaatgacat ccggtttgac caaaaacccc tgccggttct
     6601 ttccaaacag gtttcgggct ttcgactcca actcggtcaa gatctgctct tcggtgactg
     6661 catcgcggat ccttgaaagg gccaggttga cgttcggctc cgaaacggcg tagtagagcc
     6721 cgttcttttc cctgagatag aacgctttcg ttttgatgtc ctccagggcc tgctttgcct
     6781 gggcgggcgt aagccccggc tgggccacgg agagcagggc atcggattcg ttgatcccga
     6841 agacggggct cgccaatccc tccgcctggc ctgcgaggct gttcaggaag atggttttcc
     6901 aagtctggac atggtaaggg attttgtccg ggtgagggtt ttcctggtcg gcccgttcgg
     6961 cattggagcg cccaccctgg agccctgtgg tatccgtggt cccaatgtcc gcattgagca
     7021 ccgggatcat ggaggtgttg ttcgtgcggc ccaaaagctc gtccacggtc cgggaatcca
     7081 tgaagtccag gtggccggga tgaatcatcg gcacatcgtt tcccgatttc caaagggacc
     7141 gcaccgcgag agcgaggatg cgcaggacgc cacgggtttt ctggaaggtc gggacggtgg
     7201 agagcttctt gttcaacagg tccaacaagg cgggatggaa cgggtaagcg gcgcgcatct
     7261 cctcgaggaa gggctccatc tgaaggcttc ccggcagaag ggatgggttc ctctcgtaga
     7321 gctgtctgta ttcttccacg gtgcggcggg cttcttcccg gtcgatctgt tcaaaaagcc
     7381 gtttaccgag aacggcggag aggtcctttg ccttgacggg taccacgacg gtttctgttc
     7441 tcgccaaaac ggtggtcagt tctctcgcgg cgtcctgctg aagggtcagt gcctggtctg
     7501 ggtccacggg ttggctggag aggtccgaaa gggttttgga aagctccgct gtctgtgcgg
     7561 aaaaggcatc gtgaagccca gccaaggtga cgacgatggc gatgccggga tgttgggaag
     7621 cataacccag gagtgacatg aagaacgtcg caagcatggt cttggcccgg gggtaggcga
     7681 cggcccaccg ggcggcgtag tgtgccaact catcaattag gattaggact ttgcggccgg
     7741 cgaagaggct ttcgaaaaag gcttcgtcgg gggaatcgag acggtccaga taggaggtca
     7801 cttcgctgca gagttcggtt ccgccgagtt gacgggcgat ctccgtccac aggggataag
     7861 gcttcagctc ggtaccccgc gattccttga cggggacggc atctccggcg atggcgacga
     7921 tgtcgaccgt tccggggtcg ggaaggatct ctttctcgat gaattcggca acataggggg
     7981 ccaggtcctt tccctttttc gcgaggtgga ggcaggcgat gagggtgtgg gtcttgccgc
     8041 caccaaaccc agtttcaagg cgctttaggg ccgaggcggt ggtgtctccg ccgagacgga
     8101 aaacgacatc ccgaagaatc atcttcatgt cttcggtgaa atgggttcct tcggagaaaa
     8161 atgtagccgc gtccgtgtag agagggtgcc ctgatttctg tcctttgtaa aagttcgcga
     8221 cctggctcaa agaggccgtg aagatttcca tgttgataga gccggataga aggtcctttc
     8281 taggttgaca cgatttcagc acgcccacgg cagatcctcc ccgtttgtga atttactttt
     8341 ggagtacggc ttgaagctcc tcgttcgctc gctcttcatc gagtgcaaac aattccgtgc
     8401 acagcttttc cacctttccc atcatcgaaa cggtcgaaac acgagcctca gtcttggtaa
     8461 tctgattttt ggcattcact ttgatcatga ggaaaatcac gattgtctga aagtcgcatc
     8521 tgtgaacggg gtagatttcc ttggcccaaa cgccacgagt tttcgggata cctaccaggg
     8581 gagtaggacc tccctgttca acccaaccgt ttgtaagagc cttctcgcga tcgaacagtg
     8641 ctcccttcac gtccggttgc caaggtctac tttccgccag catggcgata cctcctgagg
     8701 agagattaaa gagttcgtag atatctagca catcctttgt agctgcttga ggtttcgaaa
     8761 ttgacatgca tacataacca agcctagttt agcgtcatta acgggtgctt tcaaggaaga
     8821 gcacccctaa ggatattggt tgcaacaagc gacaggataa gtcggtgaaa gaaaaaactg
     8881 taaaaaacac ccctatagtg tttctaaaat aacatccatt tccccgcagc tggagtcact
     8941 ctagggctga agagaaggaa accgtttgtg gcaacctagt ttcgaggaag ccgtaatggg
     9001 g
//
LOCUS       DAOOVC010000154         8859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4906_length_8859_cov_6.753294, whole genome shotgun sequence.
ACCESSION   DAOOVC010000154 DAOOVC010000000
VERSION     DAOOVC010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_8859_cov_6.753294"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            413..1393
                     /locus_tag="PLM79_15145"
     CDS             413..1393
                     /locus_tag="PLM79_15145"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOV87694.1"
                     /translation="MDWLDFLMVRFPVSGVETWVLLPPLVAFVVSTFTSMGGVSGAFL
                     LLPFQVSVLRFVTPAVSATNFVFNIVAIPSGVYRYVKEGRMAWPLTWVVIVGTLPGVF
                     IGYYLRVLYLPDPKAFKFFVGFVLLYIGVRLLYEMTERSQRGKKEMKMLEERFNQHAK
                     SIKGSRAARISAGLPPEAEVKTISWSLRRVEYEFWGQRFSFNTMGMFALAFVVGIIGG
                     TYGIGGGAIIAPFCVAVFHLPVYTVAGAALLGTFLTSVAGVFFYSVMPSTMPCAPDWF
                     LGLLFGVGGFGGMYVGARLQKYVPQKYLKLILGLIITFLAGSYILQYLAG"
     gene            1440..1994
                     /locus_tag="PLM79_15150"
     CDS             1440..1994
                     /locus_tag="PLM79_15150"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOV87695.1"
                     /translation="MEGSDYDLIKAVGKGDEGAFECLMRRYRKSVLNFVYRHIGDRYM
                     AEDLTQEVFLRTYLASARFVPTGKVRTWIFKIAYNLCLNEIKRMARLRRLRDELDAHQ
                     AITCESPAAGGARSGVEEVEAVLEALSRLPEPQRAALLLRAGEGLSYAEIGQVLSLSV
                     SGVESLLFRARTNLRQIVQICGKG"
     gene            2000..2413
                     /locus_tag="PLM79_15155"
     CDS             2000..2413
                     /locus_tag="PLM79_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOV87696.1"
                     /translation="MQCEQARERISAYMDGELDGPTLHQVGDHIEVCASCRALLDGFR
                     KVDDLIRGLHGLDVKDDFARQVLEKVRESDRPPGLMCPGKGALSRVLSFLEPLWELFE
                     AKRAPNTGILEEFDDFPPLSVGFIYFKFWSQVGRG"
     gene            2417..2881
                     /locus_tag="PLM79_15160"
     CDS             2417..2881
                     /locus_tag="PLM79_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOV87697.1"
                     /translation="MDVASFSLGFVAALLLVGLILGGTCVYLGRIRSGRRDVKGYLDL
                     IPDLSREQRERVQEIRRSFLPRVQTIRDNLRRERAELAGLLFEEPVDRSRIFAVAREI
                     LLHQSDLENEVIEHILEEQELLTPSQKRKFHSIIVEQFSSGGLGVHDVRGRQ"
     gene            3512..4702
                     /locus_tag="PLM79_15165"
     CDS             3512..4702
                     /locus_tag="PLM79_15165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOV87698.1"
                     /translation="METNSDDNRSDNGRRAMRFILLMGVVSLFADMTHEGARSITGPF
                     MALLGANATIVGFVAGFGELVGYLLRLPSGYLSDRTGKYWTITIIGYVINMMAVPLLA
                     LAERWEVAALLIIAERMGKAIRNPVRDAMLSHAVGEVGRGWGFGLHEAMDQVGAMLGP
                     LIVAAVFYFRGTYQTAFTVLLIPATLTIGVLLAARVLYPRPRDLESAAPDLNATGLSR
                     VYWLYLGAMVLNAAGYADFPLIGYHFQKTASVPMDWIPVFYAVAMGVDAVAALVFGRL
                     FDREGLSVLIVSALISTFFAPLVFLGGFYGGLAGMALWGVGMGAQESIMRAAIAGMIS
                     AQKRGTAYGVFNTGYGLFWFLGSALMGVLYDWSIPALVTFSMLLQLASVPLLIRVKKE
                     AGVV"
     gene            4829..5662
                     /locus_tag="PLM79_15170"
     CDS             4829..5662
                     /locus_tag="PLM79_15170"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOV87699.1"
                     /translation="MRVYFFGDIHGNLVALEACLRHLSDCRPDSIYCLGDLVGWLPFG
                     DRTLIRMSSSLSIPTVAGNHDLLVAGLFTDHPEQLDRMQATAFNAGLLSTVPGAIDYL
                     LHLPLRLETETFTVVHHSPFHLPLPGNPPTIRSFNYLDGKSLGESLKAWRSYPKRLVF
                     SGHDHVPGIYELPDMVKDPEPADVIVHRPSGGKPLKIKLKADSRYWIKAGSVGGPYRD
                     GVATANSVLYETTSETVTLFRIPYPTDELRRELASHFFVRNLPTLKRYMDLLDQGPFR
                     K"
     gene            6070..6357
                     /locus_tag="PLM79_15175"
     CDS             6070..6357
                     /locus_tag="PLM79_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87700.1"
                     /translation="MEETHEEVELKKRLPRVGQVVRSKKFGTLWRVMEKREVWQHTAD
                     DPETGDPRMVPAIYLSYWKVREGVLPGVGKMMGFVYTLHDTTFETNWEIVQ"
     gene            6680..7201
                     /locus_tag="PLM79_15180"
     CDS             6680..7201
                     /locus_tag="PLM79_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87701.1"
                     /translation="MKWMFFSYSLPARPSKARVYVWRQLKKLGAFNYQSLWIFPFSKE
                     RMNELRKLMDEIEGFKGESLLIQGKPVNRKQEEQVHKAYADSRNEEYREYIKKCEDFL
                     GEIRMETERENFTFAEVEENEEEFQKLRLWLKKIERRDLLGLPLHKAAVERIKSCEKV
                     FEDFARKVYERQS"
     gene            complement(7849..8814)
                     /locus_tag="PLM79_15185"
     CDS             complement(7849..8814)
                     /locus_tag="PLM79_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87702.1"
                     /translation="MANEKSTQVSSSLTWRPESGREPFVYQAKTPGELFGVHAGLMEK
                     TLSPEENLLYLLYSPMYEAQGGHFGIRAFPASHALAVTNRRLVVTSNRHLKAVAPEVH
                     SIPHGSVLTLEIGSALLMGWLSIRFVSETGPGCVTLIFNAAGGWEHFHAAVRTYRRAS
                     GCDRAAQGGETVPWWQVQARTPPALARSLRLLVPETEPCLGWIGSSEMWGIRKRGWKK
                     ISYCLRTNGILVAGSHGLVYIHEEPPLRRELTNFAFNALCIPLEALRSADLVENLESG
                     NRLLCLEFQLGRGSHETCVQAFLDESDMESGKRLSCVLNRHSGSG"
BASE COUNT         1811 a         2379 c         2674 g         1995 t
ORIGIN      
        1 tcaggtacaa agggtgtttt ctcagcccag atatctgagc acccccaacg gctgtcattt
       61 ttttacatca atagaatcct atccttataa gatttgcatg tcaaattgga aatttgtgga
      121 tcgaaacagc agggaggccc ttgagttgag gcgcggaatt cccgtgatgt cccgggaacc
      181 ggctggtttg ggcctcggat acgcttccca tggatgcccg ccgaaatcct atccctcagc
      241 ccagggggcc gatttgaggc gaagcccgaa tattcttcaa aggaaggcaa gattttgcgt
      301 tttcagttgt ttaccggata gatgggataa aggggttcga cgatgcgctt cgagccgtgg
      361 cccctaaata cggttccttg gacttctgca gcagtaaggg aagggagaga tcatggactg
      421 gttggatttc ctcatggtga gatttccggt ctcgggagtg gaaacatggg tattgttgcc
      481 tcccctggtt gccttcgtgg tatcgacctt cacttccatg ggcggggtct cgggggcttt
      541 cttgttatta cccttccaag tgagtgtcct gcggtttgtg actccggcag tgagtgccac
      601 gaatttcgtt ttcaacatcg tcgccatacc cagcggcgtg taccgttatg tgaaggaagg
      661 gcgcatggca tggcccctga cctgggtcgt catcgtgggt acgctgccgg gtgttttcat
      721 cgggtattat cttcgcgtac tctatcttcc cgaccccaag gccttcaaat tcttcgtcgg
      781 gtttgttctt ctttacattg gagtccggct gctctacgaa atgacggaac gttctcaaag
      841 gggcaagaag gaaatgaaga tgctggagga gaggttcaac caacacgcca agtccatcaa
      901 ggggagcagg gctgcccgga tttccgccgg gctgccgccg gaggccgagg tgaaaacgat
      961 ctcttggtct ttgcgccgcg tggaatacga gttctggggg cagcggtttt ccttcaacac
     1021 catgggaatg tttgccctgg ccttcgtggt gggaatcatc ggcggaacgt acgggatcgg
     1081 cggcggagcc atcattgccc ccttctgtgt ggcggtcttt catcttccgg tctacacggt
     1141 cgcaggggct gctctgctgg gaacgttcct aacctcggtt gcgggtgttt tcttctacag
     1201 tgtgatgcca tccactatgc cgtgcgcacc ggattggttc ctgggactgc tctttggcgt
     1261 gggcgggttt ggcggcatgt acgtgggagc cagactgcag aagtatgtcc cgcaaaagta
     1321 cctgaagctg attttgggcc tgatcatcac gttcctggcc ggcagctaca tcctccagta
     1381 ccttgccggg tgaagatttc atggctccgt cgccattcgt cgtgtgagga ttcacgtcca
     1441 tggaggggtc ggattatgac ctgatcaagg cggtcgggaa gggggatgaa ggggccttcg
     1501 agtgcctgat gaggcgatat cggaagtcgg tgctgaattt cgtctatcgg catatcgggg
     1561 accgctacat ggccgaggat ctcacccagg aagtgttcct cagaacgtat ctggcatccg
     1621 ccaggttcgt gccgacgggg aaagtccgaa cttggatctt caagatcgcc tataatcttt
     1681 gcctgaatga aatcaaacgc atggcgaggc tgcggcgact ccgggatgaa ctggacgccc
     1741 accaggcaat cacctgcgaa agcccggctg ccggaggtgc ccggagcggc gtcgaggaag
     1801 tggaagccgt cctagaggcc ttgtcccggc ttccggaacc ccagagagcc gcactgctcc
     1861 tgagagcggg agaaggactg tcgtacgccg agatcggcca ggtgctttca ctctccgtgt
     1921 ccggcgtcga gtccttgctt ttccgagccc gtacgaacct caggcagatt gttcaaatct
     1981 gcggaaaagg atgacaccca tgcaatgtga gcaagcccgg gaaaggatct cggcatacat
     2041 ggacggggaa ctcgatggcc cgaccttgca ccaggtaggc gaccacatag aggtttgcgc
     2101 ttcctgtcgc gcgcttctgg atgggttccg aaaggtggat gatctgatcc ggggacttca
     2161 cgggctggac gtgaaggacg atttcgccag gcaagtcctt gaaaaggtga gggagtcgga
     2221 taggcccccc gggttgatgt gtcccgggaa gggtgcactt tcaagggttt tgagttttct
     2281 ggagcccctc tgggagttgt ttgaggcaaa aagagctccg aataccggga tattggagga
     2341 gttcgacgat tttccgcccc tgtccgtagg gttcatctat ttcaagttct ggagccaggt
     2401 tggtcgaggt tgaagaatgg atgttgcttc cttttccctg ggttttgttg cggccctgct
     2461 cctcgtgggg ctgatcttgg ggggaacatg cgtctatctg gggcgcattc gctccggaag
     2521 gcgtgacgtc aagggatatc tcgacttgat cccggatctt tccagggaac aaagagaacg
     2581 agttcaggaa atccgccgga gcttcctccc tcgggttcaa accatacgag acaatcttcg
     2641 ccgtgagcgg gccgagttgg cgggtctttt gttcgaggag ccggttgacc gatcccgcat
     2701 ctttgccgta gccagagaga tccttctcca tcagtccgat ctcgagaatg aggtgatcga
     2761 gcacattctt gaagagcagg aactgctcac tccatcccaa aagaggaaat tccactccat
     2821 catcgtagaa cagttctcct cgggaggcct gggtgttcac gacgtgcgcg gcaggcaatg
     2881 aatccctcga tttgaacaag ccacccagca ctgcgccggg ttgaccttga cttccgcgaa
     2941 ctgagaaacc gaggctcggg tcgaggtctt cttgcccggg aggccttgta tgccgtcatg
     3001 agcgctcgga gaggctgcaa tgaagaagag tgctgatgct acggagagat gtccggatat
     3061 gcagatatga cgacgtaact atagttacaa aaatatgttg cagtagtatc tcaatccatg
     3121 tatcgtatgt ttggatgctg cgccgagctt tcacaaccgg aaatcggaaa ggagatgaac
     3181 aaggaccggt atctgggggt cgcgatggtg ctctcagggt tgtttttcgg gtggaccatc
     3241 ggctcccact acacgggagc cacgatggga atggcgatgg ctccggagcg atcaagagtc
     3301 ccatggttgc cacgggtctc attgacttct tagtcgtcct gggtgccacc tttgaaagcc
     3361 acaacgtggt gaagacggtg taagtaccgg aatcattcgg ggagaggacc ggtgattccg
     3421 gggctcgcat ggggggagct gggcagccct tatctcgaag gattcccttt tgaaaagaat
     3481 cggagtctca cggagcatca ggaggttggg tgtggagacg aactcggacg acaaccggtc
     3541 cgacaatgga cggagggcca tgaggttcat cctcttgatg ggggtggtga gtctttttgc
     3601 ggacatgact catgaaggag cgcgcagcat cacggggccc ttcatggctc tcctgggtgc
     3661 aaatgccacg atcgtgggtt tcgtggccgg attcggggaa ctggtcggat atctcctgcg
     3721 tctgccgtcg ggctacctca gtgaccggac tgggaaatac tggaccatca ccatcatcgg
     3781 gtatgtgatc aacatgatgg cggtgcccct tcttgccctg gccgagcgct gggaggttgc
     3841 ggccctcctc atcattgccg agcggatggg caaagcgatt cggaaccccg tccgcgacgc
     3901 catgctttcc catgccgtcg gggaagtcgg gaggggctgg ggcttcgggt tgcatgaggc
     3961 gatggaccag gtcggcgcca tgctcggccc cttgatcgtg gccgccgtct tttactttcg
     4021 gggtacttat cagaccgcct tcaccgtctt gctcatccct gcgacgctga ccatcggggt
     4081 gctcctggcc gcccgggtcc tctaccctcg tccccgggac ttggaatccg cggcccccga
     4141 cttgaatgcc accggccttt ctcgggtata ctggctctac ctcggggcca tggtgctcaa
     4201 cgcggccgga tatgcggact ttcctctcat cggctaccat tttcaaaaaa cggcctcggt
     4261 tcccatggac tggattcccg tcttttatgc cgttgccatg ggagtggacg ccgttgccgc
     4321 ccttgttttc gggcgcctct tcgaccgcga aggtctgtcg gtcctgatag tgtccgccct
     4381 gatatccacc ttctttgctc ccctggtgtt tctgggagga ttctacgggg gtcttgcggg
     4441 catggccttg tggggtgtgg gcatgggggc ccaggaatcc atcatgagag cggcaatcgc
     4501 cggaatgatt tcagcccaaa agcgcgggac ggcttacggg gtcttcaata ccggctacgg
     4561 gctgttctgg ttcctgggga gtgctttgat gggggtcctg tatgactggt ctatcccggc
     4621 cctcgtcaca ttctccatgc tcctccaact ggcatcggtc cctcttctca ttcgggtcaa
     4681 aaaggaggcc ggagtggtct gaagtcgctc ttgaaccgca cggatgagcc gaacttgccg
     4741 aatggaggcg agtccgcagg gtgtgccggc aaacagccat aggtttgtcg gccccctata
     4801 ggtgaaaaag tcctgggtgg agggaacgat gcgcgtctac tttttcggcg acattcacgg
     4861 aaatcttgtg gcgttggagg catgtctgcg gcatctgagc gattgccgtc cggactcgat
     4921 ctactgcctg ggagacctcg tgggatggct tcccttcgga gatcgtaccc tgattcgcat
     4981 gagttcctcc ctcagcattc cgacggtggc gggaaaccat gaccttctcg tggccggatt
     5041 gttcacggac caccccgaac aactggatcg aatgcaggca accgctttca atgccggtct
     5101 gctctcgacc gtcccgggag ccattgatta cctgctccac ttgcctctca ggctggaaac
     5161 ggaaaccttc acagtcgtgc accacagtcc gttccacctt ccgctgccgg ggaatccccc
     5221 gacgatccgg agcttcaact acctggatgg aaaatccctt ggagaatccc tcaaagcctg
     5281 gcggtcctat cccaagcgtc ttgttttcag cggtcatgat cacgtgcccg gaatctatga
     5341 actgcccgac atggtcaaag atcccgaacc ggccgacgtg atcgttcatc ggccgtccgg
     5401 gggcaagccc ctgaagatta agctgaaagc agactcccgc tattggatca aggcgggaag
     5461 cgtcggcggt ccctatcgcg atggagttgc gacggccaac tccgtcctct acgaaaccac
     5521 gtcggaaacg gttaccctgt ttcgcatccc ttaccccacg gacgaactcc ggcgtgaact
     5581 cgcttcgcac ttcttcgttc gaaatctgcc gaccctgaaa agatacatgg acttgttgga
     5641 ccaaggtcca ttccgtaagt aggcgcactt gaatctccat cgccgtcgga cccgcttacc
     5701 gcctgtgctt cgcacccgtt cttgcgagtc aatcggttgt tcaccatcat gcggcagggg
     5761 gtttacaggg acaacggaat ggagtctaat gaaagccgaa tcaacgggca acgaagtctg
     5821 ccggaatttc cggccatggg aataatgact tctatcgatg gggtagggtc gctccctcaa
     5881 gcccctaccc ccgggagatc gcgggtctgc tgggggtttt cttttttgcg cagccaaaga
     5941 caaaaggaag atggacgggc cggcaccgga gtaggcttcc ggttttgaat aaggactgga
     6001 ttggtgtccc gtggcggcgc attgccgacg gcgggtccac gggagactgc gtgccaagga
     6061 ggtagggtga tggaagaaac ccacgaagag gttgagctca agaagaggtt gcccagggtg
     6121 ggacaagtgg ttcgaagcaa gaagttcgga acactctggc gggtcatgga gaaacgggag
     6181 gtttggcagc ataccgcgga cgatccggag acgggggatc cacgaatggt cccggcaatt
     6241 tacctcagct attggaaggt ccgggagggg gtcctgccgg gagtcggcaa gatgatgggg
     6301 tttgtgtaca cgcttcacga cacgaccttt gagacaaact gggaaatcgt gcagtgacca
     6361 gtgaaggtcg cggtctccca tggttgttcg gcggggaagg ggagaatcgt ggtgggatct
     6421 ctgaaccgga agaattcgga tgccggcata tcgcaacgat ccgggctgtt cacccgcagc
     6481 gatgatcagc gacggttccg gtcatattct tgcttttgta atcattttcg ggtccatgac
     6541 gggtacgcga aggtcgccgg agaagatagg gatagggctc tccacgttta cttcctgcag
     6601 gagatgatgt aatcgtggtt cccggatgca tcgatctctt tctttcgaaa tggctggaga
     6661 ttttgcgccg gagaatgccg tgaaatggat gtttttttcc tacagtctgc ctgccaggcc
     6721 ttccaaggca agggtctacg tctggaggca actaaagaag ctgggagcct tcaactacca
     6781 gtccctttgg attttccctt tctccaagga acggatgaac gagctccgca agctcatgga
     6841 cgaaatcgag gggttcaagg gagaaagtct cctgatccag gggaagcccg tcaatcggaa
     6901 gcaggaggag caagttcaca aggcctacgc cgattccagg aacgaggaat accgggagta
     6961 catcaaaaaa tgcgaggatt tccttggaga aatccggatg gaaaccgaac gggagaattt
     7021 taccttcgcg gaagtggagg aaaacgaaga ggaattccag aagcttcggc tctggttgaa
     7081 gaagatcgaa cggagggact tgctgggctt accccttcac aaggccgccg tcgaaaggat
     7141 caagagttgc gaaaaggtct ttgaggactt cgcccggaaa gtatatgagc gacagtcgtg
     7201 ataccgggga ggtcccgatc cccttcgccc atcggcagcc tcacaacaag cagcaagcag
     7261 caagcatcac cggagagtat ggggcgcttg gcccgcaagt ccttcaccca ttgaccgagt
     7321 ttgctggaag acaccatggc gagtccaatg caaccggact gcaccggact ccggtcctgc
     7381 ccctacagaa ggccgacttg ccctgaaaac gccaacggcc cttgggtggt ttgcctggcg
     7441 gccccctccg gtgagtcgga agcctcgaac ccgtatggaa tgcacggcac cgaccattgc
     7501 gtcccatgtc cggtgccgga tgctttccgc aaagagatca agacctgtct ttatctgctt
     7561 cccatacgcc tctgggacgg atcgaacgtt cagacctttt tccattgccg actcctgtac
     7621 gagttcaacc ccaaacgcgc caacacctcc tacacctttc attcacccga attgtgtccg
     7681 tggtggtttc cccacccgaa aacgttcttg ccgccgcaaa ccgaatggca caccaatcga
     7741 gccgttggct tgtaccgggg gaagatcggg cggatcatca aaccttgtgc cccggggccc
     7801 aatgtttatc cggggaggga gtcttggtgg aaaaggcttt tgaaatgctt agccggaacc
     7861 tgaatgtcgg ttcaagacgc aggagagcct cttgccgctt tccatgtcgg attcgtccag
     7921 aaatgcctgc acgcaggttt cgtgggaacc tcggcccaac tgaaattcta ggcacagcag
     7981 acgatttcct gattccaggt tctcaaccaa gtcggcggac cgtaaggctt caagaggaat
     8041 acacaaggcg ttgaaggcaa aatttgtcaa ttcccgtctc aacgggggct cttcatggat
     8101 gtagacgagg ccgtggcttc ccgccaccag gatcccgttc gtcctcaagc aataggaaat
     8161 ctttttccaa cctctctttc gaatccccca catttcggaa gaaccgatcc aacccagaca
     8221 aggttccgtc tccggaacaa ggaggcgtag gcttcttgcc agtgcgggag gggtcctcgc
     8281 ctggacttgc caccacggca ccgtctctcc cccttgggcg gcacggtcgc acccggacgc
     8341 ccggcggtag gttcgcacgg ccgcatggaa atgttcccat ccgccggcgg cgttgaaaat
     8401 cagtgtgacg caaccgggtc cggtttcgga aacgaaccgg atggagagcc aacccatgag
     8461 caatgcactg ccgatttcca aggtgaggac ggatccgtgg gggatggagt gcacttcggg
     8521 ggcgacagct ttcagatggc gattggaggt gacgaccagc cgacgattcg tcacggccag
     8581 ggcatgggag gccggaaagg cccggattcc gaagtgcccc ccttgggctt cgtacatggg
     8641 agagtacaag aggtacagga ggttctcctc gggggagagt gtcttttcca tgaggccggc
     8701 atgaaccccg aagagttccc cgggggtctt ggcctggtaa acaaacggtt ccctcccgct
     8761 ttccgggcgc catgtcaagg atgaggaaac ctgggttgat ttctcgtttg ccatcgtcag
     8821 ccgcctccta tgccgttgtt gccggggtgt tgttcacgg
//
LOCUS       DAOOVC010000155         8758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4961_length_8758_cov_6.270252, whole genome shotgun sequence.
ACCESSION   DAOOVC010000155 DAOOVC010000000
VERSION     DAOOVC010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4961_length_8758_cov_6.270252"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..784
                     /gene="ilvN"
                     /locus_tag="PLM79_15190"
     CDS             260..784
                     /gene="ilvN"
                     /locus_tag="PLM79_15190"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOV87703.1"
                     /translation="MKHTLSVLVENAPGVLTRVAGLFSRRGFNIDSLAVGRTEVHDVA
                     RMTIVVEADDRLIEQVTKQLHKLIDVIKISDITTDEYVDRELVLVKVKADPSQRVEIM
                     QMAQIFRARVVDLGRKTLTFECTGNDAKIDAFEESLRPYGIKELVRTGKIAMLRGARF
                     AAVEGSWSEAAKES"
     gene            944..2611
                     /gene="ilvB"
                     /locus_tag="PLM79_15195"
     CDS             944..2611
                     /gene="ilvB"
                     /locus_tag="PLM79_15195"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOV87704.1"
                     /translation="MEITAAEALVRCLEHENVEVVFGYPGGAILPVYDSLHGTSGIRH
                     ILVRHEQGAVHAADGYARVTGKVGVCMATSGPGATNLVTGIANAYMDSIPLVVFTGQV
                     PTSQIGTDSFQEVDITGITIPITKHNYLVKDPQKIPSIVKNAFHIASTGRPGPVLIDL
                     PKDVMQSKIQFKYPSSVRLRGYKPTVKGFAGKIREIAQLIKKSQRPVIYAGGGIMSAH
                     AGAELLQLAETISAPVTNTFMGLSSFPGDHPLFLGMLGLHGTRYANLAVTECDLLIAL
                     GARFDDRVVMKIASFAPKAAVVHIDIDPAEIGKIVSVHVPLVGDVKSVLQDLLPQLEK
                     TDRTAWIDRIRELKEQHPLTYGKEGGLKPQFVIEHLSRLTGGKAVIVTDVGQHQMWVA
                     QYYRFIPPGILVSSGGLGTMGFGLPASIGSAVGRPQQPLILVTGDGSIQMTIQELATL
                     REQNLSFKILILNNYSLGMVRQLQEFYCDGRYMATNFGFHPDFEYLAKAYGMAGYTLK
                     TEKEVLDQLPGILDAPGPAIINCLVDAAENVSPMVLAGKGIDEAIEC"
     gene            2592..3185
                     /locus_tag="PLM79_15200"
     CDS             2592..3185
                     /locus_tag="PLM79_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV87705.1"
                     /translation="MKPSNVNRSLINFFFELGMLKKTPRSGFQFLGSGRQSVADHSFR
                     VALIGYTLARLTPEVDAFKVVCLCLFHDVPEARMGDLNYVNKKYVRADENKAVGDLAE
                     TLPFGPEYKSLLEEYRGQETEEARLVHDADQLDLILELKEQNDLGNAYAGQWIRFALK
                     RLKTPIARELAQETLHTDSAAWWFEGNDHWWVYPDGE"
     gene            3199..4653
                     /locus_tag="PLM79_15205"
     CDS             3199..4653
                     /locus_tag="PLM79_15205"
                     /inference="COORDINATES: protein motif:HMM:NF017870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HOV87706.1"
                     /translation="MKKLVSIVALVVLAALGLWVAATFWFGVKTEESYYTLLRQGSKV
                     ENLRLENESYHRGFWESKARTVLVFQPSPAASAENPPLRIHLAHDIYHGPFPFHKSVE
                     GVGPFEPVTAIIESRLVLNPEIRNQVAELYTQVPELAAVRDVTIISLDGNGEERFVIP
                     PFRRTLGREDPVVVDWKGFTLKIHFTREFKKFVGSFSAPGLEIAGENQALKIREVRSA
                     FNSEESLHGLSLGDASFSLAALEFDEKKENESTSFLMKGLSATTSARASGDTVTFLGA
                     VRADQMAFDGIQAGPGVFELEFRNLDAASLAQLQKTLDELQKHPLPGPPETVQERTLA
                     RYMEILPGLLKRSPELELKQLEVKTGDGDFTGKAMIAYDGAKSGSAVNLPALLAGVSA
                     QAEFQVGERLLRRVSAAFMKDTLIEEMEGEVEEGAGTELDDLVSARIEQQLTALTAQD
                     LIVKKDGYYGIKGAYKGGQILLNGRPLQLQELLR"
     gene            complement(4832..5200)
                     /locus_tag="PLM79_15210"
     CDS             complement(4832..5200)
                     /locus_tag="PLM79_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87707.1"
                     /translation="MTAEGDLVLVYMEGNPSFFARIEEIVPDSKPEWYQVKMLVLQVP
                     LVVITWILRSPYINGEEFTMGGRPVKLVKVVSPEGPGREEAFLGRGIEPEADPEPPGD
                     AADKGKVISLADRRKKDPSH"
     gene            complement(5184..6056)
                     /locus_tag="PLM79_15215"
     CDS             complement(5184..6056)
                     /locus_tag="PLM79_15215"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOV87708.1"
                     /translation="MNLPLLDDSHALQSLIIELLSRKCGCESLLKNEPCEGVRSSSVL
                     LLLGRKVVGPSGTREICLILNKRSRRVRQAGDLCCPGGTMEGPLDLQLARLLTLPGSP
                     LTRWPHWRHLRVHSPEEAELLSQLLATGLREGWEEMRLAPLGLRFLGPLPSQCLLAFR
                     RVIHPMVGWVSWQKRFFPSWEVEKIVWIPLRSLLDESRYARYHLYVPPELEKRFNRRT
                     QDYHCFVHPDGGTRELLWGATFRIVTNLVNLLFGFRPPPLNSLPLISGVLDEGYVYGR
                     DHETGIGGKRDRGR"
     gene            complement(6034..>8758)
                     /locus_tag="PLM79_15220"
     CDS             complement(6034..>8758)
                     /locus_tag="PLM79_15220"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HOV87709.1"
                     /translation="PGAVRDAFSVLRELGAVDDRRRLTPSGRIMARLPLDPRMARMLV
                     QANTEGALREMVILASVLSIQDPRERPLEKEAAADQKHAAFRDPRSDFVTFLKIWGAS
                     LRAEAPTPENPPEAFEPGDPFLLQEGTTPVAPPQESDVEAREATLPGGAPPEKKVGSR
                     SASRLRKFCRENFLSYRRMREWRDVHDEIRDLLEEMKGFRINERPASYEGIHRAVLSG
                     YLSHLAVRKEKNLYMGPRNRRLTIFPGSALFGKGGSWIVSADVVRTSRLYARHVATIE
                     PQWAEELGKHLCRSSYSEPHWEKDRGQVAAYERVTLYGLPIVERRKVNYHRVNPREAR
                     SIFIRSALMTGEIRERHGFLEHNGKLIEQIRELENMTRRRDLLAEEEKIYEFYDSRLP
                     DFADARSLARFVKEGGDALLRMKEEDLLQAPVDLEVMEQYPKFLASRDVRLPLRYAFQ
                     PGESEDGVTVSIPSHLLPRLRPEVFPWLVPGMLREKVTALFKALPKAVRRGLVPLAGT
                     VERVIPCLAFGEGDFFEELSRCVQKVAGVRISPDQWSREALPDHLQMRFELLDPQGRI
                     MAAGRDLPPLVSGLLGEAPDHAWDTARRRWEREGLVSWDFGDLPREIEIGTDSLGNPQ
                     YAYPMLVAEEKGVALRLCRDPIQARRETRRGLLRLVEEAFASELKQLKRQWAFPDHMN
                     AAVFFMGGRTQADRKLREFILREIFELRDPQWPDRSEFLRRVDRAKGHLAQMGGGLWE
                     EVLGVVQQRSLAQSELRRFLAMGRGNAAVQERLSLLLGELEELLPGDFLDRYGASCLR
                     HMSRYLRALTLRAERAYAAPEKDRLKSESLETHRARYRKMLEYLQDRQRDLPSLQQFL
                     EDFRWMLEEYKVSLFAPEVRTRLRVSPRRLEEKWLEWNTLKESAHESSLAG"
BASE COUNT         1784 a         2738 c         2566 g         1670 t
ORIGIN      
        1 ggggaagaaa acgacttttg gctttccaaa gctccgttgc atgagttata gatacatttc
       61 aacccggaat cgagcgattg caccctcatg tggcccccaa attcatctcc tcgcagccgg
      121 aaatccggaa aaaccaatgg tccatgggcc ggccccgggg cgggcaaaac gggggtccgc
      181 cattgtagcg gccccaaccg ggtattttgc tggtttcact gcacaactcg accgtaatgg
      241 gcaaggagga attcctgtca tgaagcacac cctctccgtc ttggtggaga atgctccggg
      301 ggtactgacc cgagtggccg ggctctttag ccggcgcgga ttcaatatcg acagtctggc
      361 cgtgggacgc acggaggttc acgatgtagc acggatgacc atcgttgtcg aggcggacga
      421 ccgtctgatc gagcaggtca ccaagcagct ccacaaactc atcgacgtga tcaagatcag
      481 cgacatcacc accgacgaat acgtggaccg ggagcttgtc ctggtgaaag tgaaagcgga
      541 tcccagccag cgagtggaaa tcatgcagat ggcccagatt tttcgggccc gggtcgtgga
      601 tttgggacgc aagaccctga cgttcgagtg tacgggaaac gacgccaaga tcgacgcctt
      661 cgaggagtcc ttgaggccgt acggcatcaa ggaactcgtc cgtacgggga agatcgccat
      721 gcttcgcggc gccaggttcg ccgccgtgga ggggtcctgg tcggaagcgg ccaaggaatc
      781 ctgaagcagg tcccgcgacg gaagattttt cccccgcttg gccctcaaac gttcgttccg
      841 cctcccgggt cccctgcgga gacgaacggg ggggagctcg attcgtccca gccaaagttc
      901 ggggtcctgg tgaccccggg aaccagtaca ggaggatacg gccttggaaa tcaccgctgc
      961 cgaagcatta gtgcgttgtc tcgaacatga aaacgtggaa gtggtgttcg gctacccggg
     1021 gggagccatc ctgcccgttt acgacagcct tcacggaact tccgggattc gccacatcct
     1081 ggtgcgccac gagcaggggg ccgtccacgc cgccgacggc tatgcccggg tcacggggaa
     1141 ggtcggggtg tgcatggcca cctcgggccc cggcgccacc aacctggtca cgggcattgc
     1201 caacgcctac atggactcca tccccctggt ggtcttcacc ggtcaggtcc ccaccagcca
     1261 gatcgggacg gattccttcc aggaggtgga catcacggga atcaccatcc ccatcaccaa
     1321 gcacaactat ttggtcaagg acccccagaa gattccctcc atcgtcaaga atgctttcca
     1381 cattgcatcc acgggacgcc ccgggccggt gctcatcgat ctgcccaagg acgtgatgca
     1441 gtccaagatc cagttcaagt acccgtcttc ggtgcggttg cgggggtaca aacccacggt
     1501 caagggattc gccggaaaga tccgtgagat cgcccagttg atcaagaagt ctcagaggcc
     1561 cgtcatctat gcggggggcg ggatcatgag cgcccatgcc ggtgccgaac tgttgcaact
     1621 ggccgaaacc atttccgcac cggtcaccaa caccttcatg gggctttcga gctttcccgg
     1681 ggaccacccc ttgttcctgg gcatgctggg actccacgga acccgctacg ccaacctcgc
     1741 cgtcaccgaa tgcgatctgc tcattgccct gggggcccgg ttcgacgacc gggtggtgat
     1801 gaagatcgca tcttttgccc ccaaggccgc cgtggttcac atcgacatcg accccgcaga
     1861 gatcggcaag atcgtttccg ttcacgtgcc cctggtggga gacgtgaaat cggtcctcca
     1921 ggatttgctc ccccagctcg agaaaaccga ccgtacggcc tggatcgacc gaatccggga
     1981 gctcaaggag cagcatcctc tcacctacgg caaagaaggg gggctcaagc cccagttcgt
     2041 catcgagcac ctcagcaggc tcacgggggg gaaggcggtg atcgtcaccg acgtgggcca
     2101 gcaccaaatg tgggtggccc aatactaccg gttcatccct ccgggaatcc tggtatcctc
     2161 gggagggctg ggcaccatgg gcttcgggct gcctgcctcc atcgggtccg ccgtcggacg
     2221 cccccagcag ccgttgatcc tggtcacggg agacgggtcc attcagatga ccatccagga
     2281 acttgccacc ctgagggagc agaatctttc cttcaagatc ctgatcctaa acaactattc
     2341 cctggggatg gttcgacagc tccaggagtt ctattgcgac ggacgctaca tggccaccaa
     2401 cttcggcttc cacccggatt tcgagtacct ggccaaggcg tacggcatgg cagggtatac
     2461 cctgaagacc gagaaggagg tcctggatca gctcccgggg attctcgacg ctcccggccc
     2521 cgccatcatc aactgcctgg tggatgcggc ggaaaacgtc tctcccatgg ttttggcggg
     2581 caagggaatc gatgaagcca tcgaatgtta acaggagtct catcaatttc ttttttgaac
     2641 tgggtatgct caagaagacg ccccgatcgg gatttcaatt cctggggtcg gggcgccaat
     2701 ccgtggccga tcattccttc cgggtagccc tcatcggcta cactctcgcc cgactgaccc
     2761 ccgaggtcga cgctttcaag gtggtatgcc tgtgcttgtt tcacgatgtt cccgaggcac
     2821 gcatgggaga cctgaattac gtcaacaaga aatatgtccg agcggacgag aacaaggccg
     2881 taggcgacct ggccgaaacc ctccccttcg gccccgaata caaatccctg ctggaagaat
     2941 acagaggcca ggaaaccgaa gaggcccggc tggttcacga tgcggaccaa ctggacctga
     3001 tcttggagct caaggaacaa aacgacctgg gcaacgccta cgccggtcag tggattcgat
     3061 ttgctctcaa acgtctcaag acccccatag ccagggaact ggcccaagaa acccttcaca
     3121 cggattccgc cgcatggtgg ttcgagggta acgaccactg gtgggtctat ccggacggag
     3181 aatgaccagg aggaccttat gaaaaagctc gtttcgattg tggctcttgt tgttctcgcg
     3241 gcgttgggac tttgggtcgc agcgaccttc tggtttgggg tcaagacgga ggaaagctac
     3301 tacaccctgt tgcgacaggg ttccaaagtc gagaatcttc ggctggaaaa cgaaagctac
     3361 caccgcggtt tttgggaatc caaagcccga acggtcctgg tgttccagcc gtccccggcg
     3421 gcttcggcgg agaacccgcc cctgcggatt cacctcgccc atgacattta ccatggtccg
     3481 ttcccctttc acaaatccgt agagggcgtc gggccgttcg agcccgtcac cgcaatcata
     3541 gagagccggc tggtgttgaa ccccgagatc cggaaccagg tcgcagagct ttacacccaa
     3601 gtccccgaac tcgcagccgt tcgagacgtc accatcattt ccctggacgg caacggggaa
     3661 gagcgcttcg tcattcctcc gtttcggcgg accctgggcc gggaagaccc ggtggtcgtg
     3721 gattggaaag ggttcaccct caagatccac ttcacgagag aattcaagaa gttcgtcggc
     3781 tctttcagcg cacccggcct ggaaatcgcc ggggagaacc aggccctgaa gatccgggag
     3841 gttcgatcgg cattcaactc cgaagagagc cttcacggat tgtccctggg ggacgcttcc
     3901 ttcagtctcg ccgccctgga atttgacgag aagaaagaaa acgagtccac ttccttcctc
     3961 atgaagggtc tcagcgccac cacttccgcc agggcatcgg gggacaccgt gacgtttctg
     4021 ggcgccgttc gagcggacca aatggcattc gatggaatcc aggcggggcc cggcgtgttc
     4081 gagctggaat tccgaaacct ggacgcagct tccctggctc agctgcaaaa gaccctggac
     4141 gaactgcaaa agcaccccct cccgggtcct ccggaaacgg tgcaggaaag gacccttgcc
     4201 cgctacatgg aaatcctgcc cggtctgctg aaaagatccc cggagctgga gctcaaacag
     4261 ttggaggtca agaccgggga tggagatttc acggggaagg ccatgattgc ttacgacggg
     4321 gccaagtccg gctcggccgt aaacctcccg gccctgttgg cgggagtgag cgcccaggcc
     4381 gaattccagg tgggagagcg gctgttgcgc cgggtatcgg cagccttcat gaaagacacg
     4441 cttatcgagg aaatggaagg ggaggtggaa gagggggccg gcaccgagct cgacgacctt
     4501 gtttccgccc ggatcgaaca gcagctgacc gcgttgacgg cccaggacct catcgtgaaa
     4561 aaagacggat attacggaat caaaggggcg tacaagggcg gccaaatcct gttgaacgga
     4621 cgtcctttgc agctccagga acttctacgg taaccggaac gccccgggga tcggatcttc
     4681 cgttttcccc ttcggatttc gcccggaaga agccgcatgg gaatcggaat tgccgccacg
     4741 accgggccca ccgccccggg ggtcccgccg gaagggcccc ttggcgcctt tggacccacg
     4801 gccgagggac ctcgtccctt cgagggcatc cctagtggga cgggtctttt ttgcgccggt
     4861 ctgcaaggga gatcaccttt cccttgtcgg cggcgtcccc ggggggctcg gggtccgctt
     4921 caggctcgat ccccctgccc aggaaagctt cttcccggcc cggtccctca ggagatacca
     4981 ccttcaccag tttcaccggt cgtcccccca tggtgaactc ctctccattg atgtacgggg
     5041 aacggagtat ccaggtgatg accaccaggg gaacctgcaa taccagcatc ttcacctgat
     5101 accactcggg cttgctgtcg gggacgatct cctcgatgcg cgcaaaaaag gacgggtttc
     5161 cttccatata caccaatacc aaatcacctt ccgcggtcac gttttccccc tatgccggtt
     5221 tcatggtctc ttccgtacac atagccctca tccaggaccc ccgagattag cggcagggag
     5281 ttcaggggtg gaggacgaaa cccaaacagc aggttcacca ggttggtcac aattcgaaac
     5341 gtggcccccc aaagaagctc ccgtgttccc ccgtccgggt gaacaaagca gtggtagtcc
     5401 tgggtacggc gattgaaccg tttctcgagt tcggggggaa catacagatg gtaccgggca
     5461 tagcgcgatt cgtcgagtaa agagcgaagg ggaatccata cgatcttttc cacttcccag
     5521 ctgggaaaaa atctcttctg ccaggaaacc catcccacca tgggatggat gaccctccga
     5581 aaggccagga ggcattgaga gggaagcggt cccaggaatc gcaggcccag gggggccagg
     5641 cgcatctctt cccagccttc cctgagccct gtggccagca actgggacaa gagttccgct
     5701 tcctccggac tatgaacacg caggtgtcgc caatggggcc agcgggtcag gggagatccg
     5761 ggaagggtga gcagacgggc cagctgcaga tccaggggcc cttccatggt tcctcccggg
     5821 caacagaggt cgcccgcctg tcgcactcgt cgcgacctct tgttgagtat gaggcagatt
     5881 tcccgggtcc ccgaaggtcc cacgactttc cgtcccagga gcaacaggac gctcgatgac
     5941 ctcacccctt cacaaggctc atttttgagg agggattcac aaccgcattt ccgggacagc
     6001 agctctatga tgagggactg cagggcatgg gagtcatcca gcaagggaag attcatgggc
     6061 ggattccttc aaagtgttcc actcgagcca tttttcctcg aggcgccgag gggatacccg
     6121 caggcgggta cggacttccg gcgcaaagag gctgaccttg tattcttcca acatccagcg
     6181 aaaatcttcc aggaattgtt gcagggaggg gaggtcccgc tgtcggtcct gcagatactc
     6241 cagcatcttt cggtatcggg cccggtgggt ctcgagggac tccgatttga gtcgatcctt
     6301 ttcgggagcg gcgtaggccc tttcggcccg gagggtcaag gccctcaagt accgggacat
     6361 gtgccggaga caagaagccc cgtatcggtc caggaaatcc ccggggagca gttcctccaa
     6421 ttctcccaac agcaaagaca ggcgctcttg aaccgctgca ttgcctctcc ccatggcgag
     6481 aaaccgccgg agttcggact gagccagact tctttgctgc accacgccca agacttcctc
     6541 ccaaaggcct ccgcccatct gcgccaggtg ccctttcgcc cggtccaccc ttcgtagaaa
     6601 ctccgaccga tcaggccatt ggggatcgcg gagttcgaaa atctccctaa gaatgaattc
     6661 ccgcagcttc cgatccgcct gggtccggcc ccccatgaag aagactgcgg cattcatgtg
     6721 gtcgggaaaa gcccactgcc tctttagctg tttgagctcg gaggcaaagg cctcttccac
     6781 caggcgaagc aatccccgcc gcgtctccct tcgggcttgg atggggtccc ggcagagccg
     6841 cagggccacc cctttttcct ccgccaccag catgggatag gcatactggg gatttcccag
     6901 ggagtcggtg ccgatctcga tctcccgggg caggtctccg aagtcccacg agacaagccc
     6961 ttcccgctcc catctgcgac gcgccgtgtc ccaggcatga tcgggggctt cccccaacaa
     7021 tccggagacc aggggcggga ggtctctgcc cgcggccatg attcgcccct ggggatccaa
     7081 cagctcgaag cgcatctgca ggtggtcggg cagggcttcc cgactccact ggtccgggga
     7141 aatgcgcacc ccggccacct tctgtacgca gcgactcagt tcctcgaaaa agtctccttc
     7201 tccgaaagcc aggcaaggaa tcacccgttc gaccgtgccc gccaggggca ccagtccgcg
     7261 gcgcacggcc ttggggaggg ccttgaaaag ggccgtaacc ttttcgcgaa gcatccccgg
     7321 caccagccac ggaaatactt ccggccgaag gcggggcagc aggtgagatg ggatggaaac
     7381 ggtcactcca tcctcactct cccccggttg aaaggcgtat cgaaggggca ggcggacgtc
     7441 ccgcgaagcc aaaaacttag gatactgctc catcacctcc aggtccaccg gggcctgcaa
     7501 caggtcctct tccttcatgc gcaggagggc gtccccccct tccttcacaa accgggccag
     7561 agaccgggca tcggcaaaat cgggcagtct cgagtcgtaa aactcgtaga tcttctcttc
     7621 ctccgcgaga agatcccggc gccgagtcat gttctccaac tcccggattt gttcgatcag
     7681 ctttccgttg tgttcgagga acccgtggcg ttcccggatt tcccccgtca tcagggcgga
     7741 acggatgaaa atggagcggg cttcccgggg attcacccga tggtagttga cctttcttct
     7801 ctccacgatg ggcaacccgt agagagtgac ccgctcgtag gccgccacct gcccccggtc
     7861 tttttcccaa tgaggttcgg aataagagga ccggcacagg tgcttcccca actcctcggc
     7921 ccactggggc tcgatggtcg ccacatgcct ggcgtacaac cgggaggttc gaaccacatc
     7981 ggcggatacg atccatgagc cccctttgcc gaagagggcc gagccgggaa agatcgtgag
     8041 cctgcggttt cggggaccca tgtagaggtt cttttccttc ctcacggcaa gatggctcaa
     8101 gtatccgctt aggacggccc ggtggattcc ctcgtaggaa gcgggacgct cgttgatcct
     8161 gaaccccttc atctcctcga ggaggtcccg gatctcgtcg tggacgtctc gccattccct
     8221 cattcgccgg taggacagga agttctcccg gcagaactta cgcagccgac ttgcgctccg
     8281 gcttcccacc tttttttccg gcggggcccc acccggcagg gtcgcctccc gggcttccac
     8341 atcgctttcc tggggggggg ccaccggagt ggtcccctcc tgtagcagga aaggatcgcc
     8401 cggttcaaaa gcctccggcg gattctccgg agtcggggcc tccgctcgga ggctcgcccc
     8461 ccagatcttc agaaaagtga cgaaatcgga tcgggggtct cgaaacgccg cgtgtttctg
     8521 atccgccgcg gcttcctttt ccaggggcct ctcccgggga tcttgaatgc tgagaaccga
     8581 cgccaggatc accatttccc tgagggcccc ttccgtgttc gcctgcacga gcattcgcgc
     8641 catccgagga tccaggggaa gccgcgccat gatgcgcccg gacggggtca gacggcgtcg
     8701 gtcgtccacc gcccccaatt cccgcaacac cgagaaagca tcccgaaccg ctccgggg
//
LOCUS       DAOOVC010000156         8719 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_4989_length_8719_cov_5.966644, whole genome shotgun sequence.
ACCESSION   DAOOVC010000156 DAOOVC010000000
VERSION     DAOOVC010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4989_length_8719_cov_5.966644"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..694)
                     /locus_tag="PLM79_15225"
     CDS             complement(65..694)
                     /locus_tag="PLM79_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOV87710.1"
                     /translation="MRVLTEFISKLAVRCLETTGYAGAAVLMALESMIAPVPSEAVMP
                     FVGFLVVDGKWNLYMAVLATSLGSVLGSLVSYSMGAYGGKPLVTRVGKYLLLDHHDLE
                     LTERFFHRRSGTITIFVSRFVPVVRHLISIPAGTGRMPLGPFLTATLAGATLWNTFLL
                     VCGMKLREHWGLVQKYSHQVDIGVAVFLFLGVLWFLRSRLSRKAGPSQV"
     gene            975..2501
                     /locus_tag="PLM79_15230"
     CDS             975..2501
                     /locus_tag="PLM79_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV87711.1"
                     /translation="MAENFSYLELRALHEVARVISRPGELKDQLQQTLDVLSSWLGME
                     RGMISVLDLQTGEAWLDVARGVDVEVDKISYQPGEGITGKVAQTGRPMAIANLKNETH
                     FLDRTGARKNLNRAELAFLCVPIFYQNRVVGVLSTDKLAHRVEDLDREVKFLEAAAEL
                     LGKVVHVRAVEEENRRLRRMLAEARRPITNIIGRSKVVQEVLRLIDQVADTNTTVLIR
                     GETGTGKELVARALHENSRRRKFPLVQVNCAAMPDTLLESELFGHEKGAFTGAVTRRR
                     GRFEEAQGGTIFLDEVGELSPIAQAKLLRVLQEREFQPLGSSRTVKVDVRVITATNKD
                     LEKEVALGTVRSDLYYRLNVFPIFLPPLRERGPDILLLADHFVLKYAREFSKEVARIS
                     TSAIDMLMSYHWPGNVRELENCIERAVLLANGEAIEAWHLPPTLQMKPAGTQNVSRGK
                     LEALVGAFERDLITDALKDVRGNQSQAARLLGTTKRIMQYKVTKYGIDPRRFRVKPQE
                     "
     gene            2673..4313
                     /locus_tag="PLM79_15235"
     CDS             2673..4313
                     /locus_tag="PLM79_15235"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOV87712.1"
                     /translation="MRVALAQINPFVGDFAGNAAKICNFVEEARRRHCDLVVFPELAL
                     LGYPPRDLLDEECFFRASRRYWKTLREAGKDIGIVCGVVSENESGFGKPYHNSALFCY
                     GGERIACAHKMLLPSYDVFDEERYFEPAQNATWVDFKGERLGITICEDIWNLAGYLPQ
                     GLYRCDPVCELRKASVDLVINISASPYHVGKTASVTQLLRTHALGGKVQVLYVNQVGG
                     NDELIFQGHSMAVDAAGNITARGADFREDLVVVDTSTLEGDIHGSELDQTSEVLEALI
                     LGLRDYSRKCGFEKVVLGLSGGVDSALTACVAVLALGADRVMGVAMPGPFNAPESLED
                     ARELAQRLGISFAVVPIGDLFAKACEVLAPVFGDLPADVTEENLQARLRGVVLMSLSN
                     KFNRLLLSTGNKSEVAVGYCTLYGDMNGGLAVLGDVPKTLVYELAWKLTRERGWIPER
                     TLVRAPSAELRPNQKDQDTLPPYEVLDAILAAYVEKRLPAAQIVAGGWDEEVVRWVIR
                     RVDRNEYKRWQAPPILRVTSKAFGMGRRFPIAQGFREV"
     gene            4374..4712
                     /locus_tag="PLM79_15240"
     CDS             4374..4712
                     /locus_tag="PLM79_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOV87713.1"
                     /translation="MKKIEAIIKPFKLDDVKETLSGLGIKGMTVTEVRGFGRQKGHTE
                     IYRGAEYVVDFLPKIKVEVVVPDNQVPEVVESIRKAANTGKIGDGKIFVIPVEECVRI
                     RTGESGEQAL"
     gene            4737..7334
                     /gene="glnD"
                     /locus_tag="PLM79_15245"
     CDS             4737..7334
                     /gene="glnD"
                     /locus_tag="PLM79_15245"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOV87714.1"
                     /translation="MVLSRLPGLLRRLKTLRQEYVDACLNDVPGLISAQAYAQTVLQH
                     LANALGDGSVSRERWCLAAVGGFGRGELSFASDLDLLFVHHQKLPATIEEIVRELTYG
                     LWDAGFEVGHSTASFSHILRLVREDFSVLTNCLEAQFVAGDRALYDQWRESFLEGFGA
                     NGRRRFMRVLQGYCEGRLRRYGESSYILEPHVKEGGGGLRDMHTIRWIAMVFRGNPSP
                     DALRECGWLTAEEKLWLDQAYDFLWRVRLQLHRMTQRRQDQLLFGEQEQMAGRLGFME
                     SEHGSAVEAFMRHYYRQSARIRRITAFVLERARESQRRTPSPGPKRRILPGPFLLEGR
                     HLQFLEPDLVGKDPRLLMTCFREAARHGAHFHHQAGSVIRENLGLWKPSMGEDPRVVR
                     QFFEILLHPQQAFDVLKVMLETGFLQTFLPEFAPLRYRVQYDVYHLYTVDEHLLRTVR
                     ELHRMEREKDEDPRLPGLCDIFVPLENRRILYLAALLHDVGKGNGKGHAARGALMVRT
                     TGARLGLLPEETDLLSFLVEHHLLLAETALKRDLMDEKPVTACAFKIGDREKLRMLYL
                     LTIADSRATGPGAWNTWKASLLRELYAKVDHVLCRGNGKGEDLRRRSEESQARVLRLA
                     QEDPRIEKIRAWLDRVSYRYLASQEPESMLIHYGMEQRVDRETVVLETRQAEGGLWQI
                     TLAVQDRPGLFALITGVLWLRGLNILSADIYTRPYGVALDVLMVEGLPDPLHAQELWE
                     RIRADLVEALHDRTGLDARLAAKKRPSILHQRTLPKKEDRVVIDEDASDFHTVIEVYT
                     WDRPGVLHAVTQTLYENRVSIRLAKISTPGAQVADVFYVTDLGGSKLGSSQSGDRIRE
                     ALLECLARV"
     gene            7739..>8719
                     /gene="glnA"
                     /locus_tag="PLM79_15250"
     CDS             7739..>8719
                     /gene="glnA"
                     /locus_tag="PLM79_15250"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOV87715.1"
                     /translation="MTPKEVLAFAKENGAKMVDFKFMDFVGTWQHFSNPISELSESDF
                     ENGYGFDGSSIRGWQPIHASDMLILPDPATATMDPFTAVPTLTMICNVVDPVTKEKYT
                     RDPRHIAQKAEAYLKFTGIGDTAFIGPEAEFFIFDDIRFDDASQFGYYFIDSVEGIWN
                     SGRMENPNLGYKPRHKEGYFPVPPVDSLQDLRTEMVLVMEQVGIAVECQHHEVATAGQ
                     AEIDMKAAPLVQMADWLQWYKYVIKNVARRNGRTVTFMPKPLFGDNGSGMHTHLSIWK
                     DGRPLFAGDKYAGLSEMALHAVGGVLKHAAALCAITNPTTNSYKRLVPGYE"
BASE COUNT         1756 a         2645 c         2729 g         1589 t
ORIGIN      
        1 cagaagggtt gctccaaccg gtcgagtccc ggagtgagcg atggtctccg ggccctctcg
       61 gcggtcaaac ttgagaaggt cccgcctttc gcgacaggcg cgatcgcaaa aaccagagaa
      121 cgcctaggaa caagaaaacc gcgactccga tatccacctg gtgggaatat ttctggacga
      181 ggccccaatg ttcccggagc ttcattccgc agacgagaag aaaagtgttc cacaaagtgg
      241 cccccgccag ggtggccgtg aggaagggcc ccaggggcat tcggccggtg cccgcgggaa
      301 tggaaatcaa gtggcgaacg accggaacga accgactcac gaagatggtg atggtgccgc
      361 ttcgtcggtg aaagaacctt tcggtgagtt ccaggtcgtg atggtccagg agcaggtact
      421 tgcccacccg ggtgaccagg ggctttcccc cgtaagcgcc catagaatag gaaaccaggg
      481 aacccaggac ggaacccagg ctggtggcta agaccgccat gtaaaggttc cacttgccgt
      541 ccaccacgag aaagcccaca aagggcatca ccgcttcgct gggaacggga gcgatcatgc
      601 tctccagggc catgagcacc gccgctcccg catatccggt cgtctccaga caccggaccg
      661 ccagtttcga aatgaattcc gtcagcaccc tcatatctcc tacgcttgaa gtggaggcac
      721 agcccctcct ctgcctttcg atcggctgaa taataactgc attcgttcgg gtccacaatg
      781 aattctttcc cgggaggggg aagggcgggg cgcacggggc aaagggggtt cggccgctcg
      841 atgagtgagg ggttcagatg gggagggaag tttggtgaca tttttgtgct gatccggtaa
      901 ataaaacccc ggtactggtt ttccaacggt ggactgaacc ctcatgggtc gaactttgac
      961 tacgggaacg gaacatggca gagaatttta gctacctgga gctccgggcc ctgcacgagg
     1021 tcgcccgggt catttcgcgt cccggcgagc tgaaagacca gctccagcag accctggacg
     1081 tgctcagttc ctggctggga atggaacggg gcatgatctc ggtcctggac cttcagacgg
     1141 gggaagcctg gctggacgtg gcccggggag tggacgtgga agtcgacaag atttcctacc
     1201 agccggggga ggggatcacg ggcaaggtgg cccagaccgg tcgtcccatg gccattgcca
     1261 atctcaagaa cgagacccat ttcctggacc gcaccggggc ccgaaagaac ctgaacaggg
     1321 ccgagctggc ttttctttgt gtccccatct tctatcagaa ccgggtggtg ggggtccttt
     1381 ccacggacaa gctggctcac cgggtcgagg acctggaccg ggaggtgaag ttcctggaag
     1441 ccgcagccga gctcctgggg aaagtggttc atgtccgggc cgtcgaggag gaaaaccggc
     1501 gcctgcgaag aatgctcgcc gaagcccgga ggcccatcac caacatcatc gggcgatcca
     1561 aggtcgtcca ggaagtcttg cgcctcatcg accaggtggc ggacaccaac accacggttc
     1621 tcattcgtgg agaaacgggc accggaaagg aactggtggc cagggccctc cacgagaaca
     1681 gccgccgcag gaaattcccc ctggtccagg tcaactgcgc ggccatgccg gacacgcttc
     1741 tcgagagcga gctctttgga cacgaaaaag gggctttcac gggtgccgtg acccggagga
     1801 gagggcgctt tgaagaggcc caggggggga ctatcttcct ggacgaagtg ggggaattgt
     1861 ctcccatcgc ccaagccaag ctgcttcggg tcctccagga acgggaattc cagcccctgg
     1921 gctcgtcgcg caccgtgaag gtggatgttc gggtcatcac ggcaaccaac aaggacctgg
     1981 aaaaagaagt cgccctggga acggtgcgat ccgacctgta ctaccgcctg aacgtctttc
     2041 ccatcttcct gcctcccctc cgggaacggg ggccggacat cctcctgctg gcggaccatt
     2101 tcgtgctcaa gtacgcacgc gagttctcca aagaggtggc ccgcatctcc accagcgcca
     2161 tcgacatgct catgtcctat cactggccgg gcaacgtccg ggaactggag aattgcatcg
     2221 aacgcgccgt gctcctggcc aacggggaag ccatcgaggc ctggcacctg cctcccaccc
     2281 tgcagatgaa gcccgcaggc acccaaaacg tcagccgtgg aaagctcgaa gccctggtgg
     2341 gcgccttcga aagggacctc atcaccgacg cgctgaagga tgttcgaggg aaccaatccc
     2401 aggcggcccg cctcctgggg accaccaagc ggattatgca gtacaaagtg accaagtacg
     2461 ggatcgaccc gcggcgtttt cgcgtcaaac cccaggaatg atccccgaga ggaggctctc
     2521 ggcatcgggg gcgacgggcc gctgcgggga agggatcgag atgggatcgc atctggcccg
     2581 aatcctgcat attctagagg ctcctcgttc ccgtcgattg ctcctataac cgacggccgt
     2641 gacggccgta ccctggatcc aaaggacgaa ccatgcgcgt ggctctggct cagatcaatc
     2701 ctttcgtggg ggattttgcg ggcaacgccg ccaagatctg caacttcgtc gaagaggctc
     2761 gccgacggca ttgcgatttg gtggtttttc ccgaactggc tctcctggga tacccgcccc
     2821 gggatctctt ggatgaagag tgcttctttc gtgcctcccg gcggtactgg aagaccctcc
     2881 gggaagccgg caaggacatc ggcattgtgt gcggggtcgt gtcggaaaac gaatcgggtt
     2941 ttggcaagcc gtatcacaat tcggccctgt tctgctacgg cggagaacgc atcgcctgcg
     3001 cccacaagat gcttcttcct tcctacgacg tcttcgacga ggaaaggtac ttcgaacccg
     3061 cccaaaacgc cacctgggtg gatttcaagg gagaacggct gggcatcacc atctgtgaag
     3121 acatctggaa ccttgccggg tacctgcccc agggactgta tcgatgcgac cccgtttgcg
     3181 agctccggaa agcctccgtg gacctggtga tcaacatttc ggcgtccccc tatcacgtgg
     3241 ggaagaccgc ctcggtgacc cagcttctca ggacccatgc cctcggggga aaagtccagg
     3301 tcctctacgt caaccaggtg gggggcaacg acgaactcat cttccagggc cacagcatgg
     3361 ccgtggacgc cgccgggaat atcacggccc ggggagcgga cttccgcgaa gatctcgtgg
     3421 tggtggacac ctcgactctc gagggggaca tccacggctc cgaactcgat cagacctccg
     3481 aagtcctcga agcgctcatc ctgggccttc gggattactc ccgcaaatgc ggtttcgaaa
     3541 aggtggttct ggggttgagc ggaggggtgg actcggccct gacggcctgc gtggcggtcc
     3601 tggccctggg agcggaccgg gtgatggggg tggccatgcc cggtcccttc aacgccccgg
     3661 agagcctcga ggatgcccgg gagctggccc aaaggctggg aatttccttt gccgtggtcc
     3721 ccatcggcga tcttttcgca aaagcctgcg aggtgctggc ccccgtattc ggggacctgc
     3781 ctgccgacgt caccgaggaa aatctccagg cccggcttcg cggcgtcgtc ctcatgtccc
     3841 tgtccaacaa gttcaaccgg ctgctcctca gcaccgggaa caagtccgaa gtggccgtgg
     3901 gttactgcac cctctacggc gacatgaacg ggggactggc cgtcctgggc gacgtcccca
     3961 agacccttgt gtacgaactg gcctggaaat tgacccggga gcgcggctgg atccccgagc
     4021 gcaccctggt tcgagcgccc tcggcggaac tgcgtcccaa tcaaaaggac caggacactc
     4081 ttccgcccta tgaagtcctg gacgccatcc tcgccgccta cgtggaaaag cgcctgccgg
     4141 cggcccaaat cgttgccggg ggctgggacg aagaggtggt gcggtgggtg atccgccggg
     4201 tggatcgcaa tgaatacaag cgatggcagg cgcctcccat cctgagggtg acctccaagg
     4261 ccttcggaat ggggcggcgg tttcccattg cccagggatt tcgggaggtg tagacgggcc
     4321 tccttcgagg cccggcgggt gcgggcggcg gttcaagagc ggagaacggc actatgaaaa
     4381 agatcgaggc aatcatcaag cccttcaagt tggatgacgt caaggagacc ctctcgggtc
     4441 tggggatcaa gggaatgacg gtcaccgaag tgcgcggctt cggccgtcag aaaggccaca
     4501 cggagatcta ccgcggggct gaatatgtgg tggattttct tcccaagatc aaggtcgagg
     4561 tggtggtgcc cgacaaccag gtccccgaag tggtcgaatc catccgcaag gccgccaaca
     4621 ccgggaaaat cggggatgga aagatctttg tgattcccgt ggaagagtgc gtgcggatcc
     4681 gaacggggga gtcgggggaa caggccctct agacctcgaa gcccacaagg aaacgaatgg
     4741 tactgagccg ccttcccggc ctcctgcgcc gactcaagac cctgcggcag gaatatgtgg
     4801 atgcctgcct caacgacgta cccggcctca tcagtgccca ggcctatgcc caaaccgtcc
     4861 ttcagcacct ggcgaacgcc ctgggggacg ggtccgtctc ccgggagcga tggtgccttg
     4921 ccgccgtggg aggattcggc cggggagagc tcagcttcgc ctcggacctg gacctgctgt
     4981 ttgtccacca tcagaagctt cccgcgacga tcgaggagat cgtccgggag ctcacctacg
     5041 gcctgtggga cgccggtttc gaagtggggc attccacggc gtctttctcc catattctaa
     5101 ggctggtgcg ggaagacttc tccgtcctga ccaattgcct ggaagctcaa tttgtggcgg
     5161 gggaccgggc cctctacgat caatggaggg aatccttcct cgagggattc ggcgccaacg
     5221 gccgccggcg tttcatgagg gttctgcagg gttactgcga gggaagactg cgccggtacg
     5281 gggagtcttc ctatatcctc gaaccccatg taaaggaggg gggtgggggg cttcgggaca
     5341 tgcacaccat tcggtggatc gccatggtct tccgggggaa cccgtccccg gacgccctcc
     5401 gggaatgcgg ctggctcacc gccgaagaaa agctttggct ggaccaggcc tacgactttc
     5461 tctggagggt gcgcctgcag ctgcaccgga tgacccaacg gcgccaggat cagctcctct
     5521 tcggggaaca ggaacaaatg gccgggcgcc tggggtttat ggaaagcgag cacggctccg
     5581 ccgtggaggc gttcatgcgc cactactacc ggcagtctgc ccggatccgc cgcatcacgg
     5641 cctttgtcct cgagcgggcc cgggaatccc agcggaggac cccttccccg ggtcccaaga
     5701 gacgaatcct ccccgggcct tttcttctgg aaggtcgtca tcttcaattc ctggaacccg
     5761 acctggtggg caaagacccc cggctcctca tgacgtgttt ccgggaagcg gcccggcacg
     5821 gcgcccattt tcaccaccag gccgggagcg tcatacggga aaacctgggc ttgtggaagc
     5881 cttccatggg agaagacccc cgggtcgtac gacagttctt cgagatcctt cttcatcccc
     5941 aacaggcctt tgatgtcctc aaggtcatgc tggaaacggg attcctccag acctttcttc
     6001 ccgaattcgc ccccttgcgc taccgggtcc agtacgacgt gtatcacctc tataccgtgg
     6061 acgagcacct gttgcgcacg gttcgagagt tgcaccggat ggaacgggag aaggacgagg
     6121 acccccgtct ccccgggttg tgcgacattt ttgtgccgct cgaaaatcgt cgaatcctct
     6181 atcttgcggc gcttcttcac gacgtcggga agggcaacgg gaaaggacac gcggcccggg
     6241 gggccctcat ggtgcgcacg acgggggcaa ggctggggct cttgccggag gagacggatc
     6301 ttttgtcctt tctcgtggag catcacctgc tcctggcgga gacggccctc aagcgggacc
     6361 tcatggatga aaagcccgta accgcctgtg cgttcaagat cggagatcga gaaaagctgc
     6421 ggatgctcta ccttctgacc attgccgatt cgcgggccac gggccccggg gcctggaata
     6481 cttggaaggc ttccttgctc agggagctct acgccaaggt cgatcacgtt ttgtgccgag
     6541 gaaacgggaa gggcgaggac ctgcgtcgac ggtcggagga gtcccaggcc cgggtgctcc
     6601 ggctcgccca agaagacccg cgaatcgaaa agattcgggc ctggctggat cgagtgtcct
     6661 accggtacct ggcctctcaa gaacccgaat ccatgctgat ccactacggg atggagcaga
     6721 gggtcgaccg ggaaacggtg gtgctggaaa cgcggcaggc agaagggggc ctgtggcaga
     6781 tcaccctggc cgtgcaggac cggccgggcc tgtttgccct catcaccggg gtcctttggc
     6841 tgcggggcct gaacatcctg tcggcggaca tctacacccg cccgtacgga gtggccctgg
     6901 acgttctcat ggtggaaggc ctgccggacc ctctccacgc tcaagagttg tgggagcgga
     6961 ttcgcgccga cctggtcgag gccctccacg accgaaccgg cctcgatgcc cggctggcgg
     7021 ccaagaaaag gccgtccatc ctccatcaga ggaccctgcc gaagaaagag gaccgggtgg
     7081 tgatcgatga agacgcttcg gacttccata ccgtcatcga ggtgtacacc tgggaccgcc
     7141 cgggagtcct ccatgccgtc acccaaaccc tgtacgagaa ccgggtgtcc atccgcctgg
     7201 ccaagatctc gacgcccgga gcccaggtgg ccgacgtctt ctatgtcacg gacctgggcg
     7261 gcagcaaact gggaagctcc cagtccgggg accggatccg ggaggccctc ctggaatgcc
     7321 tggcccgggt atgaagccga gcccgcccgc ggcccggcat tcctcgatct cggtacaatc
     7381 ttgtaccctt tctgtcgagt gcttgtgcgg cccgcagggt gccgaggagg cggatttttc
     7441 gggccttatc ccaggtcgat ccccttcatt ccggctgaat ttcttttcca tgcggcacat
     7501 ttttgttctt tagtgaaaag ccgggtcgac atgcccattg ttagcgccaa tgaaaggttc
     7561 ggtggatgaa acggcttccg gggccgttcc gtgaggaaat tgcaggaacc ggcggccgtt
     7621 ggcaagcaaa ttgcctggac atcccggcga gcctctttca tcgcagagga cgatcccggt
     7681 ggttgaaacg atcccacaca acgacggacc ggcaacagaa accttaggag gatgaagcat
     7741 gacacccaaa gaagtcctgg cctttgccaa agagaacggc gccaagatgg tggatttcaa
     7801 attcatggat tttgtcggca catggcagca tttcagcaat cccatctcgg agctgagcga
     7861 atcggacttc gagaacgggt acgggttcga cgggtcgagc attcgaggct ggcagcccat
     7921 tcatgccagc gacatgctga tcctccccga tccggccacg gcgaccatgg accccttcac
     7981 ggcggtcccc accctcacca tgatctgcaa cgtggtggat cccgtgacca aggaaaaata
     8041 cacgagagac ccccgccaca ttgcccagaa ggccgaagcc tacctaaagt tcacgggcat
     8101 tggggatacg gcgttcatcg gtcccgaggc ggaattcttc atcttcgacg acatccgctt
     8161 cgatgacgct tcccagttcg gctactactt catcgattcc gtggaaggaa tttggaacag
     8221 cgggagaatg gagaacccga acctgggcta caagccccgc cacaaggaag ggtatttccc
     8281 cgtgcccccc gtggactccc tccaggacct gcgcaccgaa atggtcctgg tcatggagca
     8341 ggtggggatt gccgtcgagt gtcagcatca tgaagtggcc actgccgggc aggcggaaat
     8401 cgacatgaag gcggcgcccc tggtccagat ggccgactgg ctccagtggt acaagtacgt
     8461 catcaagaac gtggcccggc ggaatggaag gacggtcacc ttcatgccca agccgctgtt
     8521 tggagacaac gggtccggca tgcacaccca cctgagcatc tggaaagacg gcaggcccct
     8581 ttttgccggg gacaagtacg cgggactgag cgagatggcc cttcacgccg tcggaggggt
     8641 gctcaagcac gctgcggccc tgtgcgccat cacgaacccc acgaccaact cctacaagcg
     8701 gctggtcccc ggttacgaa
//
LOCUS       DAOOVC010000157         8707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5000_length_8707_cov_6.704808, whole genome shotgun sequence.
ACCESSION   DAOOVC010000157 DAOOVC010000000
VERSION     DAOOVC010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8707
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_8707_cov_6.704808"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLM79_15255"
     CDS             complement(<1..276)
                     /locus_tag="PLM79_15255"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699828.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOV87716.1"
                     /translation="MGEKSFRVAVVGATGAVGNQMIRVLEERGFPVGEIKLLASYRSV
                     GKKLPFKGQDIEVEELKESSFRGVQAALFSAGGGISRKFAPIAARSGC"
     gene            complement(320..1390)
                     /locus_tag="PLM79_15260"
     CDS             complement(320..1390)
                     /locus_tag="PLM79_15260"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOV87717.1"
                     /translation="MARKTYRIAVIPGDGTGPEVVREALKALQAVMDREDFRAELVEY
                     DLGGSRYLRTGEVLPASVLDELRTVHAILLGAIGHPEVKPGILEKGLLLELRFQLDQY
                     INLRPVILYPGVDTPLKNKGPEDINFVVVRENTEGLYTGSGGFLRKGTPYEVAVQESI
                     NTRSGVERCVRFAFEYCRKRNQRKKLTLCGKTNVLTFAFDLWDRVFHETAREYPDIRT
                     DYAHVDATCMWMVKNPEWFDVIVTDNMFGDIITDLGAMIQGGMGIAAGGNLNPQGVSM
                     FEPIGGSAPKYTGRNIINPLAAVGALQMLLEHLGEGSAAQRVEGAVRKVVARDLKSLS
                     AGEMGYTTQEVGDLVADHIAKS"
     gene            complement(1444..1950)
                     /locus_tag="PLM79_15265"
     CDS             complement(1444..1950)
                     /locus_tag="PLM79_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOV87718.1"
                     /translation="MKLKGYAWKFGDDVDTDAIIPARYLNTTDPEILAAHCMEDADPE
                     FPAKVRKGDILVAGKNFGCGSSREHAPIAVKASGVGCVIAHSFARIFYRNAFNMGLLI
                     LECPEAAESIVTGDEIEVDPDRGVIVNHTQNKTYHAQPIPPFMQDLVRAGGLMPYVKE
                     QAQKQSGA"
     gene            complement(2007..3266)
                     /gene="leuC"
                     /locus_tag="PLM79_15270"
     CDS             complement(2007..3266)
                     /gene="leuC"
                     /locus_tag="PLM79_15270"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOV87719.1"
                     /translation="MPQTIGEKILAAHAGKDRVHPDELIEVRVDFALGNDITAPLAIQ
                     AFRKAGATRVFDPARIALIPDHFVPNKDIPSAVQAKEVREFAQEQHLVHYYEVGRMGI
                     EHALLPELGLVLPGDVVIGADSHTCTYGALGAFATGVGSTDLAAAMITGKVWFKVPHS
                     VKFVYRGKPRPWVGGKDLILYTIGKIGVDGARYRAMEFTGEVIGSLPMADRFSMCNMA
                     IEAGGKAGIIAPDALTEEYVRGRARREYRIVRADPDARYEAVYDWDISDLDPVVALPH
                     SPANVRMVSELPDISIHQAVIGSCTNGRLEDLRLAARVLRGRKVAPGVRCIIIPATQR
                     IYLDALNEGLLEVFIQAEAAVSTPTCGPCLGGYMGILAPGERCVATTNRNFVGRMGHP
                     QSEVYLSNPAVAAASAVAGRIVHPDAL"
     gene            complement(3401..4243)
                     /gene="pssA"
                     /locus_tag="PLM79_15275"
     CDS             complement(3401..4243)
                     /gene="pssA"
                     /locus_tag="PLM79_15275"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOV87720.1"
                     /translation="MSAEVKKRRPRKRKWKRWKEGEFHRGTYIVPNLFTTANLFAGFF
                     GIVSAINGRYEVAAVAVLISCVFDILDGKVARLTGATSRFGVEYDSLADLVSFGVGPG
                     LLMYLWALKPFGRLGWLAAFVFVACGALRLARFNVQVEVVNKKYFVGLPIPGAAGMVS
                     TSVLFLNDWLVPGSAASTAPLLVATYLLGFLMVSTVPFHSFKDLEITKTKPIAVIFVS
                     IVIVTIIAIHPELMLFLMISAYVLSGPVLFIVHKIKSPQPNPSPEVPVESRPDVGERE
                     ERAP"
     gene            complement(4228..4893)
                     /locus_tag="PLM79_15280"
     CDS             complement(4228..4893)
                     /locus_tag="PLM79_15280"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOV87721.1"
                     /translation="MNARDFRDKAAHIPVAREGVPYLVGAMFVTLTTAVLGHTILTWL
                     GLAVVLLMGHFFRDPERIGTPGETEALSPADGKVLDVERVEDAHFFSRPSTKISIFMS
                     IFDVHVNRVPLSGTVRGISYEKGSFMAAGRARASRENERNWLWIEAETGHDVVLTQVA
                     GLIARRIVCWPRVGDRVIAGERFGLIRFGSRVDVYLPETVEVTVRKGQHVYAGETVLC
                     RLR"
     gene            complement(5099..6100)
                     /gene="ilvC"
                     /locus_tag="PLM79_15285"
     CDS             complement(5099..6100)
                     /gene="ilvC"
                     /locus_tag="PLM79_15285"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOV87722.1"
                     /translation="MRIFYEADADLNVLRGKKVAVIGYGSQGHAQAQNLRDSGVDVIV
                     GELEGTANYQHALEDGFKPVPAREAAAAGDIIQILTQDHLQALIYRQDLAPSMKAGKT
                     LLFSHGFNIHYGQIVPPKEADVIMVAPKGPGHLVRSEYVRGAGVPSLVAVFQNPSGHA
                     METALAYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVTELIKASFDTLVEAGY
                     QPEIAYFECMHELKLIVDLLYRGGMAFMRYSVSDTAEYGDLTRGKRIVTEQTRAEMKK
                     ILNEVQTGAFAKEWILENQAGRPMFSTLREIEKRHLIEEVGSKLRSMMPFLEPVKID"
     gene            complement(6259..6759)
                     /gene="ilvN"
                     /locus_tag="PLM79_15290"
     CDS             complement(6259..6759)
                     /gene="ilvN"
                     /locus_tag="PLM79_15290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOV87723.1"
                     /translation="MDTKNKDRHTIGILVQNVPGVLARVVGLFSGRGFNIECITASET
                     HEPGLTRITLVTTGDENILSQIVKNLNKLINVIKVYDFTDIEFVDREMALIKVRAEQH
                     TRAEVLRIVDIFRAKVVDVSPHFYTLEVTGNESKISAIMELLGQMGIVEVARTGKAAL
                     ARSKKH"
     gene            complement(6773..8491)
                     /gene="ilvB"
                     /locus_tag="PLM79_15295"
     CDS             complement(6773..8491)
                     /gene="ilvB"
                     /locus_tag="PLM79_15295"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOV87724.1"
                     /translation="MKLKGAQIFFECLKREGVEVIFGFPGGSVLDIYHEMPKSGIRHV
                     MVRHEQAAAHMADGYARASGRVGVCLVTSGPGATNTVTGVATANMDSVPIVVFTGQVP
                     TAMIGDDAFQEVDIIGITRPCTKHSYLVKDVNDLARVIREAFYLARTGRPGPVVVDLP
                     KDVIQAVTEFKYPKKVDLPGYRPNLEAHAGQIKRAYQLIKKAKSPVIYAGGGVILSGS
                     SEELTRFAQMIQAPVTATLMGLGCFPAVIPGEKGQMIPNPLWLGMLGMHGTYRANMAV
                     THSDLLLAVGARFDDRVTGKIAGFAPNAKIVHIDVDPTSISKNVPVDIPIVGDCRNAL
                     QKLISLAEAEPVENLESVRAPWLDQVGEWGRKFPLSYEQGPDLIKPQFVVEKIYEVSK
                     GDAIITTEVGQNQMWTAQFFHFNRPRTFLSSGGLGTMGYGFPAAVGAQVAFPDRLVVD
                     IAGDGSIQMNIQELITVVCNNLPVKVAILNNRYLGMVRQWQELFYDKNYSSTCLDSSP
                     DYVKLAEAYGAVGFRAAKPEDVEPVLREAFSVRRPVLMDFLVDPTEGVYPMVPAGKSI
                     SEMLLD"
BASE COUNT         1674 a         2686 c         2522 g         1825 t
ORIGIN      
        1 gcagcccgac cgtgccgcaa tgggagcgaa tttcctgctg atgccccctc ccgccgaaaa
       61 cagggctgcc tgcacccccc ggaaagagga ttccttgagc tcttccacct cgatgtcttg
      121 ccccttgaag ggaagcttct ttcctaccga tcggtacgat gccaggagct tgatctcccc
      181 tacgggaaac ccgcgttcct ccaatacccg gatcatctgg ttgcccacgg ctcccgtggc
      241 cccaactacc gctacgcgga atgatttttc acccatcgct cgtctctttc tcgatcatcc
      301 tgaggtttcg atgccgtctc tacgacttgg cgatatgatc tgccacaagg tctcccactt
      361 cctgggtcgt gtaccccatt tctcccgcgg aaagcgattt caaatcccgg gcgaccacct
      421 tccgaacggc gccttccacc cgctgggcgg cggaaccctc ccccaagtgt tcgagcaaca
      481 tctggagagc gccgacggcg gccaggggat tgatgatgtt cctgcccgta tacttgggcg
      541 ccgatccgcc gatgggctcg aacatggaca ccccctgggg attgaggttg cctcccgcgg
      601 caatgcccat cccgccctgg atcatggctc ccaggtccgt gatgatgtcg ccgaacatgt
      661 tgtcggtgac gatcacgtcg aaccactccg ggttcttcac catccacatg cacgtggcat
      721 ccacgtgggc atagtccgtc cgaatgtccg gatattcccg cgccgtttca tggaagaccc
      781 gatcccagag atcgaaggca aaggtcaata cattggtctt gccgcacaga gtcagtttct
      841 tgcgctgatt ccgtttgcgg caatactcga aggcgaatcg cacgcaccgt tcgacccccg
      901 aacgggtgtt gatggattcc tggaccgcca cctcgtaagg cgttcccttc ctgagaaacc
      961 ccccggaacc cgtgtacaga ccttccgtgt tttcccgcac caccacgaag ttgatatctt
     1021 cggggccctt gttcttcagg ggagtgtcca ctcccgggta cagaatcacc ggccgaagat
     1081 tgatgtactg atccagttgg aagcgaagct cgaggagaag ccccttttcc aggattcccg
     1141 gcttgacttc cgggtgtccg atggcaccca gcaaaatggc gtgaacggtc ctcagctcat
     1201 ccagtacgct cgccgggagg acctcgcccg tacgaaggta gcgactcccc cccagatcgt
     1261 attccaccag ttccgcccgg aagtcttccc ggtccatcac cgcttgtagg gccttcaaag
     1321 cctcccggac aacctccggt ccggtcccgt cccctggaat cacggcaata cgataggtct
     1381 tacgtgccat ggagcaccct cttctcgtgt ctgtgggttt cgtggttcac gggtcctttg
     1441 gaatcaggct cccgattgct tttgggcctg ctccttcaca tagggcatga gtcctcccgc
     1501 ccggacgaga tcctgcataa acggcgggat cggttgggcg tgataggtct tgttttgtgt
     1561 atggtttaca atgactccgc ggtccggatc cacctcgatt tcatctcctg tgacgatgga
     1621 ttccgccgcc tcggggcact ccaggatgag gagccccatg ttgaacgcat tccggtagaa
     1681 gatccgggca aagctgtggg cgatgacgca gccgactccc gaggccttga cggcaatggg
     1741 ggcatgttcc cgggaggaac cgcagccgaa gtttttgccg gccaccagga tatccccttt
     1801 tcgcaccttg gcggggaatt cggggtcggc gtcttccatg cagtgggctg ccaggatctc
     1861 ggggtccgtg gtgttcaggt atcgagcggg gatgatggcg tcggtatcta cgtcgtcccc
     1921 aaacttccat gcatagcctt tcaatttcat agattcatcc tcgctgggca gacggttgca
     1981 ggggaaaccc gttgggccga gccgcgttag agggcgtcgg ggtggacgat gcggccggcc
     2041 acggccgaag ctgcggccac cgcggggttg ctcaggtaca cctcgctctg gggatgtccc
     2101 atgcgcccca cgaaattccg attggtggtg gccacgcagc gctctcccgg ggccagaatt
     2161 cccatgtatc cgcccaggca cggcccgcag gtcggggtgc tcacggcggc ttcggcctgg
     2221 atgaagacct cgaggaggcc ctcgttgagg gcgtccaggt agattctctg ggtcgccggg
     2281 atgatgatgc agcgcacccc cggggccacc ttcctccccc gaaggacccg ggcggccagg
     2341 cgcaggtcct cgagcctgcc gttggtgcag cttccgatga ccgcctgatg gatggaaatg
     2401 tcgggcagct cgctcaccat ccgaacgttg gccggggaat gaggcagggc caccacggga
     2461 tcgagatcgg agatgtccca atcgtagacc gcctcgtagc gggcatccgg gtcggcacgc
     2521 acgattcggt actcccgccg ggcacggccc ctcacatatt cttcggtaag ggcgtccgga
     2581 gcgatgatcc ccgcctttcc ccccgcttcg atggccatgt tgcacatgga aaagcggtcg
     2641 gccatgggca gagacccgat cacttcgccg gtgaattcca tagcccggta tcgggccccg
     2701 tccacaccga ttttgccgat ggtgtaaaga atgaggtcct tacctcccac ccagggtctg
     2761 ggtttgcccc ggtagacgaa ctttacggaa tggggcacct tgaaccagac ctttccggta
     2821 atcatggcgg ccgccaagtc ggtgcttccc actcccgtgg cgaaagcccc cagggccccg
     2881 tacgtacagg tgtgactatc cgccccgatg acgacgtccc cgggaagaac caggcccagc
     2941 tcggggagca gcgcgtgctc gatgcccatc ctgcccactt catagtagtg gaccaggtgc
     3001 tgctcttggg cgaactcccg gacctccttg gcctggacgg ccgaggggat gtccttgttg
     3061 ggtacgaagt gatcggggat gagggcgatg cgggcggggt cgaaaacccg ggttgcgcca
     3121 gcctttcgaa acgcctggat ggccaggggt gccgtgatgt cgttgcccag ggcgaaatcc
     3181 acccggacct cgatcaactc gtcggggtga acccgatctt tccctgcatg agctgccaga
     3241 attttttctc caatggtctg aggcattcct ttccctttcc acaaccaggg gctgccggtt
     3301 gctcgtcacc cccttgggca agaatgacgc atgcaccctt taggggtccc gtgtgacaac
     3361 attttcacaa tccacaaaag accgataacc gacaggagca ctagggtgcc ctttcctctc
     3421 gttcgccgac gtccggccgg gattcgacgg ggacctcagg ggagggattc ggttgtgggc
     3481 ttttgatctt atggacgatg aacaaaacgg gcccggacaa cacataagcc gatatcatca
     3541 ggaaaagcat cagttccggg tggatggcaa tgatggtcac aatgacaatg ctcacaaaaa
     3601 tgacggcgat gggcttggtc ttggtgattt ccaggtcttt gaaactgtgg aagggaacag
     3661 tgctcaccat gagaaaaccc aaaaggtagg tggccacaag aaggggcgcc gtggacgcgg
     3721 cggacccggg gaccagccag tcgttcagga agagcaccga ggtcgacacc atgccggcgg
     3781 ccccgggaat gggcaggccc acgaagtatt tcttgttcac cacttccacc tggacgttga
     3841 agcgcgccaa tcgaagagcg ccacaggcca caaacacgaa ggccgccagc catccgagtc
     3901 gaccgaaggg cttgagggcc cacaggtaca tgagaagacc ggggcccaca ccgaagctca
     3961 ccaggtcggc cagggaatcg tactcgaccc cgaagcgact ggtggccccc gtcaggcggg
     4021 ccactttccc gtccagaatg tcgaaaacgc aggagatgag cacagctacg gccgccactt
     4081 cgtagcgtcc gttgatggcg ctgacgatcc cgaagaaccc ggcaaaaaga ttggccgtgg
     4141 tgaagaggtt ggggacgata taggtccccc ggtggaactc cccttccttc catcgcttcc
     4201 attttcgctt tctgggacga cgcttcttca cctcagccga cataggaccg tctcccccgc
     4261 gtagacatgc tgcccctttc gcacagtgac ttccacagtt tccggaaggt agacatccac
     4321 tcgggagcca aagcgaatca gcccgaagcg ctcccccgca atcacccggt cccccacccg
     4381 gggccagcag acgattcggc gggcaatcaa ccccgccacc tgggtcagga ccacgtcatg
     4441 gccggtttcc gcctcgatcc agagccagtt tctttcgttt tcccgggaag ctcgggcgcg
     4501 gccggcggcc ataaaagacc ccttctcata ggaaatcccc cggacggttc ccgaaagggg
     4561 aacccgattc acatggacat cgaagatgga catgaagatg ctgattttcg tggagggccg
     4621 ggaaaaaaag tgggcgtcct cgacccgctc gacatcaagg actttgccgt cagcggggga
     4681 gagcgcctcg gtctctcccg gggtcccaat cctctcgggg tcccggaaga agtgccccat
     4741 gagcaggacc accgccaggc ccagccaggt aaggatcgtg tgtcccaaaa cggcggtggt
     4801 gagggtgacg aacatcgccc caaccaggta gggaactcct tccctggcta cggggatgtg
     4861 ggctgccttg tctcgaaagt cccgggcatt caacgaacgt tacggctcct tcgcagcaca
     4921 gaagtctcgg gagcaaccca tcgaggtctc cgggtgagga gggcccccac ccgggaaatc
     4981 tcgatgcggt cgccgtttgg ctatccccaa caagcgctcc ctggtcgtgc cgctccgggc
     5041 gggtgccgat ggggagtccc gttgccgggg aatgacccgg tgcggcgtct catgaaggct
     5101 agtcgatctt taccggttcc aggaacggca tcatggatcg gagtttgctt cccacttcct
     5161 cgatgaggtg tctcttttcg atttccctca aggtactgaa catggggcgc ccggcttggt
     5221 tctcgaggat ccattccttg gcgaaggccc ccgtctgcac ctcgttcagg attttcttca
     5281 tttcggcccg ggtctgttcc gtgacgattc gttttccccg ggtcagatcg ccgtattcgg
     5341 cggtgtcgct gacggagtag cgcatgaagg ccatcccgcc gcgatacaga aggtccacga
     5401 tcagcttcag ttcgtgcatg cattcgaaat aggcgatctc cggttgatac ccggcttcca
     5461 ccaaggtgtc gaagctcgcc ttgatgagct cggtaacccc tccgcacaga accgcctgtt
     5521 ctccgaaaag gtccgtctcc gtctcttcct tgaaggtggt ctcgatgacg cccgctcggg
     5581 tggcgccgat tcccttggca tacgccaggg ccgtctccat ggcgtgaccg ctgggattct
     5641 ggaatacggc caccagagaa ggcactccgg ctccccgcac gtactcgctc cggaccaggt
     5701 gccccggtcc cttgggagcc accatgatga cgtccgcctc tttgggaggc acgatttgcc
     5761 cgtaatggat gttgaagccg tgagaaaaca gaagggtctt gccggctttc atggaagggg
     5821 ccagatcctg gcgatagatg agcgcctgca ggtgatcctg ggtcaggatt tgaatgatgt
     5881 cgccggcggc ggccgcctct cgcgcgggga ccggtttgaa accgtcctcg agggcatgct
     5941 gatagttggc cgtcccctca agctctccta cgatcacatc cacaccgctg tctctcaggt
     6001 tctgagcctg tgcatgccct tgacttccat acccgatgac cgccactttc ttgccgcgga
     6061 gcacattcaa atcggcgtcc gcttcgtaaa aaattcgcat gacaccctcc ttacgggact
     6121 cgatgatggg aagaggttcc gccccatccg ggttttctta ccgtccccaa acggggccaa
     6181 gcctcaaaca ggcgacggga ccgcctgccc ccctgcgggg caggcctgcg ggggattccc
     6241 cgcggattcc atccgatgtc aatgcttctt gcttcgggcc agggccgcct ttcccgtgcg
     6301 ggcaacctct acgatgccca tttgaccaag aagctccata atcgccgaga ttttgctctc
     6361 gttgccggtg acttccagcg tatagaaatg ggggctcaca tctaccactt tcgcccgaaa
     6421 aatgtcaacg atgcgcagga cctccgcccg ggtatgctgt tcggcccgca ccttgatcag
     6481 ggccatctcc cggtctacga actcgatgtc ggtgaaatcg tagaccttga tcacgttgat
     6541 caacttattg aggttcttca cgatttgaga gaggatattc tcgtcgcccg tggtcaccag
     6601 ggtgatccgc gtcagtccgg gctcatgggt ttcggatgcg gtaatgcact cgatgttgaa
     6661 gcctcttccg ctgaaaagtc caaccactcg agcgagaacc cccggcacgt tctgcaccag
     6721 gatgcctatg gtgtgtcgat ccttattctt tgtgtccatg cgcgcttcca ggttagtcga
     6781 gtaacatttc cgaaatgctc tttcctgcgg gcaccatcgg atacactcct tcggtgggat
     6841 caaccaggaa atccatcaag accggtcgtc gaacggagaa ggcttcccga agcaccggtt
     6901 cgacgtcttc gggtttcgct gcacggaagc ctacggcccc gtaggcttcg gccagcttca
     6961 catagtccgg ggatgaatcc aggcaggtgc tgctgtaatt cttatcgtag aagagctcct
     7021 gccattggcg caccatgccc aggtagcggt tgttcaggat ggccaccttc acggggagat
     7081 tgttgcacac caccgtgatg agctcctgga tgttcatctg aatgctgccg tcccccgcaa
     7141 tgtccaccac cagccggtcc ggaaaggcca cttgggcccc gacggccgcc ggaaatccgt
     7201 atcccatggt ccccaatccg ccggaactga ggaaggtgcg gggacggttg aagtggaaga
     7261 attgagccgt ccacatctgg ttctgaccca cttccgtggt gatgatggca tctcccttgc
     7321 tcacctcgta aatcttttcc accacgaact ggggcttgat gaggtcgggc ccctgttcgt
     7381 aggaaagagg gaacttgcgc ccccattctc ccacttgatc cagccagggg gcacgaacgc
     7441 tctcgaggtt ctcgacgggc tcggcttcgg ccagggagat cagcttttgg agggcattcc
     7501 tgcagtctcc cacgatgggg atgtccacgg gtacgttctt gctgatgctg gtggggtcca
     7561 catcgatatg aacgatcttg gcgttgggcg caaatccggc gatctttccc gtcacccggt
     7621 cgtcaaaccg ggcgcccacg gccaggagca ggtccgaatg ggtgacggcc atgttggcgc
     7681 ggtaggtgcc gtgcatcccc agcattccca accagagcgg gttggggatc atttgtcctt
     7741 tctcgcccgg gatgacggcc ggaaaacaac ccaaccccat aagggtggcg gtgaccggcg
     7801 cctggatcat ttgggcgaag cgggtaagct cctcggaaga ccccgagaga atgacccctc
     7861 cgccggcgta tatcaccggg gatttggctt tcttgatgag ttgatacgcc ctcttgatct
     7921 gcccggcatg ggcctcgaga ttgggtcgat atcccggcaa atccaccttc ttgggatact
     7981 tgaattccgt gacggcctgg atgacatcct tgggcaaatc caccacgacg ggtcccgggc
     8041 gccccgttcg ggcgaggtaa aacgcctccc gaatcactcg ggcaagatcg ttcacgtcct
     8101 tgaccaagta gctgtgtttg gtgcaggggc gcgtgatacc gatgatgtcc acttcctgaa
     8161 aggcgtcgtc tccgatcata gccgtgggca cttgcccggt aaacaccacg atggggacgg
     8221 aatccatgtt ggccgtagcc actcccgtaa cggtgttggt ggccccgggt cccgaggtga
     8281 ccaagcagac tccgaccctt cccgaggctc gggcgtaccc gtcggccatg tgagccgccg
     8341 cctgttcatg acgaaccatg acgtggcgga ttccggactt gggcatctcg tggtagatgt
     8401 ccagaacgga acctcccgga aatccgaaga tcacttccac gccttctctt ttgagacatt
     8461 caaagaagat ttgagctccc ttaagcttca tacgcgtccc caatcatccc ccggtcctgc
     8521 tcttgtgagg atgccgccga cggtcggcat catttacatt ggtgaaactt tgtttaatct
     8581 aagcaatttt gactagatag agaggtgatt gtaaaaaaat cgcgtcgagc tgtcaacgta
     8641 tggaatgggg ccggggaaga aaacgacttt tggctttcca aagctccgtt gcatgagtta
     8701 tagatac
//
LOCUS       DAOOVC010000158         8697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5005_length_8697_cov_8.060056, whole genome shotgun sequence.
ACCESSION   DAOOVC010000158 DAOOVC010000000
VERSION     DAOOVC010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5005_length_8697_cov_8.060056"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLM79_15300"
     CDS             <1..1001
                     /locus_tag="PLM79_15300"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOV87725.1"
                     /translation="SMLPKVPTMKQTPIETAPADLAELAAILERICFKMQDDQAQQWG
                     ASRPLVEQMSRVRELSGPILERLRASSRETRDRVQAYRERFQEDLLKTWDFFRNAASA
                     PPMTMDDLPPVLKGWFYHDGVFLLRAYPKESVWEEDALSTFVRQLQAVEPQVVGDPVS
                     LHVFASAFRKACLLASIYAVIAVLILLVLTFRRLSLALTAMVPLVAGTLWTVAVMGLA
                     GVDFNLANSIFMPLVVGAGVEYAVVILHRWQEGNIEAGHLPVSTAKGVIGAALTTTVG
                     FGTLMISNHQGIFSLGFVAWAGSIFVLLAALLLLPALLIAVYPASPEGRKERVETP"
     gene            998..1723
                     /locus_tag="PLM79_15305"
     CDS             998..1723
                     /locus_tag="PLM79_15305"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV87726.1"
                     /translation="MIPKHFHQFMVWKKRIVREGLRGRRALARGRLSSRGTLLNRIAF
                     LWILAALSMIPAVTEAAPAPQAATSQVKAVMDDAMNIQTRPDLEGEAHRRERARLIRK
                     LIADNFLAGDMARESLKGHWDRISTKQRTEFQRLFTVLFQDSYTRLVLNFLKKETVEY
                     RGESTVEKDTVVRTVIMRANEHIPVDYHMVRKSGRWFIRDVDIDGVSIVQNYRNKFRQ
                     VIEKTSVDGLLQNMKLQSQATEQ"
     gene            complement(1828..2280)
                     /locus_tag="PLM79_15310"
     CDS             complement(1828..2280)
                     /locus_tag="PLM79_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV87727.1"
                     /translation="MKEIRKILFPVDLSDVSPKIVPNVMSVAEKFGAEIHLLHVAIDF
                     DDHAGLFGPHPGLADVQEDILRRSREQLDEFHQTHFKEYPRILKAIQCGDPVEKILEY
                     VASEGIDLVIMGTHGRKGLDKVLFGSVADQVVKNSPVPVMSVNPYRTL"
     gene            complement(2504..3649)
                     /gene="hpnI"
                     /locus_tag="PLM79_15315"
     CDS             complement(2504..3649)
                     /gene="hpnI"
                     /locus_tag="PLM79_15315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HOV87728.1"
                     /translation="MIQDIFRYGFALLVVCSLGYFGVAFFAARKFFRRGGPDASELPP
                     ASILIPLHGSDVEAYENYASFCLQDYPRYQIVFGVTDPEDPSVPLVRKLGEDFPQVDL
                     ELVVGTERIGRNPKVNNLWNIYRKTKYDRLVLVDSDIRVERDYLRRVVPCLNDGRTGL
                     VTCLYRGRTAPNWASGLEAVGITGEFAPGVLVAWLIQGIRFAFGATIAVTREALESIG
                     GLKAVADYLADDYMLGHLVARKGYGVELSPCIVETMLPPVSFSDMLRHQIRWGRAIKA
                     GRKGGYLGSALIHGTALALLNALLWAGAPSALLLLFLAVGAQMALGWYVGVHFMKDRI
                     LKRYLFLLPLRDLLGLAVWCMVFCGNRVVWRGKTFRISSDGKMTPSS"
     gene            complement(3646..4014)
                     /locus_tag="PLM79_15320"
     CDS             complement(3646..4014)
                     /locus_tag="PLM79_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV87729.1"
                     /translation="MMTMLMIGIIVLSNAAGDVFITKGMKQIGEIPLTKPRRLWLTAG
                     KVATNRNFLLGLCLLAVSFAAFLAVLSWEDMSFIVPATSVVYVVTILGARFILKEHID
                     GLRWLGTCLVCAGVALMCLP"
     gene            complement(4054..4485)
                     /locus_tag="PLM79_15325"
     CDS             complement(4054..4485)
                     /locus_tag="PLM79_15325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOV87730.1"
                     /translation="MKTAITIALAVLAQAAGNTLLSKGMKGIASLGKLQESISPMLIL
                     DVLGNPLIWIGTLCLGLFFALFSLSLSWEDLSLVLPATSIGYVVNVAFAHYFLQEPVS
                     LSRWAGTGLIVAGVILVSRSAAQAKQRESEETLPRSGLGMS"
     gene            complement(4482..5351)
                     /gene="hpnK"
                     /locus_tag="PLM79_15330"
     CDS             complement(4482..5351)
                     /gene="hpnK"
                     /locus_tag="PLM79_15330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HOV87731.1"
                     /translation="MKPAKKLILNADDFGASERVNRAVLRAFEHGILTSCSLMASGPA
                     FDHAVRIAGDHPRLAVGIHLVTVMGKSVLPHVRIPHIVDRNGNFRQNPVWAGLWYQFS
                     GKARQDLRREIEAQFEKFASTGLRLSHVDSHLHMHVHPVLFQMALDLGIQFGAGAMRV
                     PADDLAAFERHYGKASPGARAQWTVFRILTRRMKQKLAEKGFAFCTRVYGHLGSGRME
                     ESYVLGLLDRLPPGAHEIYVHPALLDAGDRPTPDRLQGQTELDILLSPRVKERIRSLD
                     ILAATYKDLEVTA"
     gene            complement(5443..6864)
                     /gene="hpnJ"
                     /locus_tag="PLM79_15335"
     CDS             complement(5443..6864)
                     /gene="hpnJ"
                     /locus_tag="PLM79_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HOV87732.1"
                     /translation="MKTLLLNPSSFEKFDAGASSRWPARREISSFWYPVWLAYPAGMI
                     RESRLLDAPSHGVSPEQTIQLAKDYEFLVVFTSTPGFENDIRLAEAVKDLNPRISVAF
                     VGPHVSVLPEETLRATDAIDFVVRKEFDDALTEFASGKKLEDIAGISFRSNGQIHHTP
                     DRPLLLDLDRLPFAVDTYKRDLDITRYSVPFLLHPFVAFYTARGCPARCTFCLWPQTF
                     SGHRWRTRSTESVVAEFKRAYELFPGLQEIFFDDDTFTWGKSRVLEICEKLKPLKKTW
                     SCTCRVHADYETLKAMKEAGCRLLIVGFESASSTILRNVKKGATAEQGRELMKNCRRL
                     GIAVHGDFIIGLPGETQETIRETIRFAEEIDCETIQVSIAHAFPGTEMYQYLRDRGYF
                     PDGVMADELGHQLPNFEYPGLSRREIMDAVEYFYGRYYFRPRVIFRIVSGAIFDGAKR
                     RRLIQEAREYLQLRARRKKFIRS"
     gene            7326..>8697
                     /locus_tag="PLM79_15340"
     CDS             7326..>8697
                     /locus_tag="PLM79_15340"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87733.1"
                     /translation="MSPNRSQLGLSNLKPGDHLCWVYETDEEHRSVLIPFLSQGVEND
                     EKILFIWDVSSAEDTVRYLRNDSMDVEACLCRGQLSIITAKEGYMRGGFFDPEAMIRL
                     LERESAFAQAQGYSVLRVAIAPPSMPHGLPVHKHFADFETKMNVFMRRQCCLMLCMYD
                     RRRYSSQMLLDMLNSHPVVVLGTSFYENFYYVPPKDLLGVDAATAALRDRLQSLALRK
                     HAEELLRISEANYRAIFNAANDAILLLDMETGAIIDANEKTYEMWGYSPEEFLRLNVE
                     DLISEEPPYQRGDGIRRIEKAAHGIPQLFEWVFKNKVGKRFWGEVNLKRAFNEEKNFV
                     LAVVRDITERKWADKLLRESEERFRSIFHNVYVGVALVDKQGHVLDANEAFCGFLGYS
                     PRELIGMHLSRFTFEEELNEEMQLLDELCSGKRPTHVMDKRYLRKDGEVVWGQLSLSA
                     IRDNEGM"
BASE COUNT         1841 a         2507 c         2595 g         1754 t
ORIGIN      
        1 gctccatgct gcccaaggtc cccaccatga agcagacgcc catcgagacc gctcccgcgg
       61 acctggcaga gctcgcggca atcctggaaa ggatctgttt caaaatgcag gacgaccagg
      121 cacagcaatg gggggcatcc cgtcccctcg tggaacagat gagccgggtg agggagcttt
      181 cgggcccgat cctggagagg ctgcgggctt cctcccggga aacccgggat cgggttcagg
      241 cataccggga gcgttttcag gaggacctgc tcaagacctg ggattttttc agaaacgccg
      301 catcggctcc tcccatgacc atggacgacc tgcctcccgt gctcaagggc tggttctatc
      361 acgacggggt gtttctgctt cgcgcctatc ccaaggaatc cgtctgggaa gaagacgccc
      421 tcagcacctt tgtccggcaa ttgcaggcgg tggaaccgca ggtggtggga gaccccgttt
      481 ccctgcacgt gtttgcatcc gcttttcgca aggcatgcct cctggcctcc atctacgcgg
      541 tgattgccgt tttgatcctg ctggtcctga ccttccgtcg cctttccctg gccttgacgg
      601 ccatggttcc cctggtggcg ggaaccctct ggacagtagc cgtcatgggc ctggcaggag
      661 tggacttcaa cctggcaaac agcatcttca tgcccctggt ggtgggagcg ggggtggaat
      721 atgcggtggt gattctgcac cgatggcagg agggaaacat cgaggcgggg catttgcccg
      781 tgagcacggc caagggggtg atcggtgccg cattgaccac caccgtgggt tttggtactc
      841 tcatgatctc gaaccaccaa gggattttca gccttggttt cgttgcctgg gccggcagta
      901 tcttcgtcct ccttgctgct ctgcttctat tgcctgcctt gttgatcgcc gtgtaccccg
      961 catcgcctga agggagaaag gagcgagtgg aaaccccatg attcccaagc atttccacca
     1021 gttcatggtt tggaagaaac ggatcgtccg ggaaggtctt cgaggtcgca gggctcttgc
     1081 ccggggccgt ctttcttcga gggggactct tttgaaccgg attgccttcc tttggatcct
     1141 cgcagccttg agcatgattc ccgcggtgac cgaggccgcc cccgctcccc aggcagccac
     1201 ctcccaggtg aaagcggtca tggacgatgc catgaacatc cagacgcgac ccgacctcga
     1261 gggagaggcc caccgccggg agcgcgctcg actcatccgc aagttgatcg cggacaattt
     1321 cctggccgga gacatggccc gggagtccct caaaggccat tgggaccgga tctccacaaa
     1381 acagcggacg gaattccaac ggctcttcac ggtcctcttc caggattcct acacacgcct
     1441 ggtgctcaac ttcctcaaga aagagaccgt cgaataccgg ggggaatcca ctgtcgagaa
     1501 ggacaccgtg gttcgcacgg tgatcatgag ggccaacgaa cacattcccg tcgattacca
     1561 catggtccgg aagagcggcc gatggttcat tcgggacgtg gatatcgacg gagtgagcat
     1621 tgtccagaac tacaggaaca agttccggca agtcatcgag aagacttccg tggacgggct
     1681 ccttcaaaac atgaaactac agagccaggc cacagagcag tagaagcggg agggagttct
     1741 tccgtccaac gtccggggag gaaccgggac ggattgcaga cgcttttcgc ccaaagggcg
     1801 cgtcctccgg gaattgccga taccttgtta aagggtcctg taagggttga cgctcatgac
     1861 gggtacgggc gaatttttga ccacctggtc ggcgacgctg ccgaagagca ccttgtccag
     1921 ccctttcctg ccgtgggtac ccatgatgac gagatcgatc ccctcggaag cgacgtactc
     1981 caggattttt tccacgggat ctccgcactg aatggccttc agtatccggg gatactcttt
     2041 gaagtgggtt tgatggaatt catccagttg ttcccgggag cgtcggagaa tgtcttcctg
     2101 gacatccgcc aggcccggat ggggaccaaa aaggcccgcg tggtcgtcaa agtcgatggc
     2161 cacgtggaga agatggatct ccgctccgaa tttctccgca acggacatga cgttggggac
     2221 aatcttgggg gagacatccg acagatccac cggaaatagg attttgcgta tttctttcat
     2281 aagcccctcc tttccgaagg tttcccttcg agtccgtatc ggcaatcccc ggtgaggatc
     2341 tttgagcgcc gccgttttgt ctcgcaatgg gaaaaatcga tggtttcatc ttgatggtaa
     2401 gaagagaatt cccgttggtc aaccgagggg ttacgggggc cgggaggatc tttcgggaca
     2461 atctcgacgg gccggttcgt ggggctaaac cccggaaatc ccttcacgac gagggggtca
     2521 tttttccgtc cgaggatatg cgaaaggtct ttcctctcca caccaccctg ttcccgcaaa
     2581 agaccataca ccaaacggcc aagccgagaa ggtccctcaa gggcagcagg aaaagatacc
     2641 gcttcagaat ccggtccttc atgaagtgga ctcccacata ccatcccaag gccatctgcg
     2701 cccccacggc gaggaacagc agcagaaggg cggatggcgc ccctgcccac aagagggcgt
     2761 tcagtagggc cagggcggtc ccgtggatga gggcgcttcc caggtaaccg cccttgcgac
     2821 cggccttgat ggcacgaccc catcgaatct ggtgcctgag catatccgaa aaggaaaccg
     2881 ggggcagcat ggtctccacg atgcacgggg aaagctccac cccgtaacct ttccgggcaa
     2941 cgagatgccc cagcatgtag tcgtcggcga gataatcggc aacggctttc aaccccccga
     3001 tggactcgag agcctcccgg gtgacggcaa tggtggctcc gaaggcgaat cgaatcccct
     3061 ggatgagcca ggccacaagg actccggggg caaattcccc ggtgattccc accgcttcaa
     3121 gtccggacgc ccaattgggc gcggtccttc cgcgatacag gcaggtgacc agccccgttc
     3181 gcccatcgtt caggcagggc accacgcgcc tcaggtagtc tctctccacc cggatatcgc
     3241 tatccaccag cacaagccga tcgtacttgg ttttccggta gatgttccaa aggttgttta
     3301 ccttgggatt tcgtccgatc cgctcggtac cgaccaccag ttccaagtcc acctgaggaa
     3361 aatcttctcc cagtttccga accaaaggaa cggatggatc ctcggggtcc gtgaccccga
     3421 agacgatctg gtatcggggg taatcctgca ggcagaagga ggcatagttc tcgtaagcct
     3481 cgacgtcgga cccgtggagg ggaatcagga tgcttgccgg agggagttcc gatgcatcgg
     3541 gtcctccacg ccggaagaac ttccgagccg caaagaaagc aaccccaaag tagcccaggg
     3601 aacagaccac caggagagcg aaaccgtagc ggaaaatatc ctggatcatg gcaggcacat
     3661 gagggcgacc ccggcgcaaa ccagacaggt tcccagccat cgcaggccgt caatgtgttc
     3721 cttgaggatg aaacgggctc ccaaaatggt caccacgtac acaacggaag tggccgggac
     3781 gatgaagctc atgtcttccc aggacagaac agccaggaac gcggcaaaac tgacggccag
     3841 caggcaaagc cccaacagaa agtttcggtt ggtggccact ttccctgcgg tgagccacag
     3901 gcggcggggt ttcgttaagg ggatctcgcc gatctgtttc atccccttgg tgatgaaaac
     3961 atccccggcc gcattggaca gaacgatgat cccgatcatc agcatcgtca tcattgggag
     4021 cggttcctat tccggttctt ccgtaaagag ggttcaagac atgccgagcc cggaacgcgg
     4081 cagtgtctcc tccgattccc gttgctttgc ttgggccgcc gaccgggaca cgaggatcac
     4141 cccggccacg atcaatcccg tcccggccca gcgcgacaag gaaacgggtt cttggagaaa
     4201 atagtgggca aacgccacat tcaccacgta tccgatggaa gtggccggga gtacgaggct
     4261 caggtcctcc caggaaaggg agagactgaa gagggcaaag aaaagaccca ggcagagcgt
     4321 cccgatccat atgagggggt ttcccaggac atcgagaatc agcatgggag agatggattc
     4381 ctggagcttc ccgagggagg cgatgccttt catgcccttg ctgagaagag tgtttcccgc
     4441 tgcctgggcc aagacggcca gggctattgt gatggcggtt ttcatgcggt cacttccagg
     4501 tccttgtagg tggcggccag gatgtccaaa ctgcggattc gttcctttac cctcggactc
     4561 aggagaatgt ccagttccgt ttgtccctgc aaccggtccg gggtcggccg atcccccgca
     4621 tcgagcagag ccggatgcac gtagatttca tgggctccgg gtgggagccg gtccaggagg
     4681 cccaggacat aggattcctc catgcggccg ctccccaaat gcccgtatac cctcgtgcag
     4741 aatgcaaaac ccttttccgc gagcttctgc ttcatgcggc gcgtcaagat ccggaacacg
     4801 gtccattggg cccgggcacc gggggaagcc ttcccataat ggcgctcaaa ggcggccagg
     4861 tcgtcggcgg ggaccctcat ggccccggcg ccgaattgaa ttcccaggtc caaggccatt
     4921 tggaacagga ccgggtgcac atgcatgtgg aggtgactgt ccacatggga aagccggagt
     4981 cctgtggagg cgaatttctc gaattgggcc tcaatctctc tgcgaaggtc ttgcctcgct
     5041 ttcccggaaa actggtacca caagcccgcc catacggggt tttggcgaaa gttcccgttt
     5101 cggtcgacga tgtgggggat ccggacatgg ggcaagacgg attttcccat caccgtcacc
     5161 aggtggattc ccacggccag ccggggatgg tctccggcaa tccggacggc gtggtcaaag
     5221 gccggtcccg aggccatcag gctgcagctc gtgaggatcc cgtgctcaaa ggctcgaaga
     5281 accgcccggt tcacccgttc ggaggcgccg aaatcgtccg cattgaggat aagctttttg
     5341 gcgggtttca tgaagtatct cagggcctcg gcggtgaaat gggcaggatt cgtcggcgct
     5401 gcggctggtt cccatggggc cgtctctcgg gggtcgggtc cctcaagagc gaatgaactt
     5461 ctttctcctg gctcgaagct ggaggtattc ccgggcttcc tggatgagcc tcctgcgctt
     5521 ggctccgtca aagatcgccc cgctcacgat gcggaaaatc acccggggac gaaagtaata
     5581 ccgtccgtaa aagtactcca ccgcatccat gatctcccgc cgggagagcc ccgggtactc
     5641 gaagttgggc aactggtggc ccaattcatc cgccatgacc ccgtccggaa agtaaccgcg
     5701 gtcccggagg tattggtaca tctccgtgcc cggaaatgcg tgggcgatgg aaacctggat
     5761 ggtttcgcag tcgatttcct cggcaaaacg aatggtttcc cggatggttt cctgagtttc
     5821 cccgggaagt ccgatgatga aatctccgtg caccgcgatg cccagccggc ggcagttctt
     5881 catcagctcc ctgccctgct ccgccgtggc tccctttttg acgtttctga ggatagtgga
     5941 gctcgcggat tcaaacccga cgataagcag tcggcatccc gcctccttca tggccttgag
     6001 ggtctcgtag tcggcgtgga cgcgacaggt acacgaccag gtcttcttca agggcttcag
     6061 cttttcgcag atttcgagca cccgggactt gccccaggtg aaggtgtcgt cgtcaaagaa
     6121 aatctcctgc aggccgggaa agagctcgta ggcccgttta aactcggcga ccacgctttc
     6181 ggtgcttcgg gttctccatc gatggccgct gaaggtttgg ggccaaaggc aaaaggtgca
     6241 ccgggcggga catccgcgtg ccgtataaaa ggctacgaag ggatgaagca aaaatggtac
     6301 actatatcgg gtaatatcca ggtctcgctt gtaggtgtcc acggcgaagg gcagacgatc
     6361 caggtcgagg agcagggggc gatcgggagt gtggtggatt tggccgttgc tgcgaaagct
     6421 gattcctgcg atgtcctcca acttttttcc cgaagcgaat tccgtcaatg catcatcgaa
     6481 ttccttgcgc accacgaagt caatggcgtc ggtagctcgc agcgtttctt cgggaagaac
     6541 ggaaacgtgg ggtccgacaa aggcgacgct gatgcgggga ttcagatcct ttacggcctc
     6601 cgccaacctg atgtcgtttt cgaaaccggg cgtactggta aataccacca ggaattcata
     6661 atccttggcc aactgtatgg tttgctcggg gcttaccccg tgggaaggag catccaagag
     6721 cctgctttcc cggatcatac ccgctggata tgccagccag acaggatacc agaaggatga
     6781 aatctccctt cgggcaggcc acctcgaact ggcgcctgca tcgaattttt caaaggaaga
     6841 gggattgagc agcagcgttt tcatggagcc tccgttggat cggtcttttg aggggagcac
     6901 tatacagggg gacaaaatcc cctgtcaagg gaggcggcat ggagggttaa ggaagagcgc
     6961 ttcatcccgg gggagacggt tcgaaggatt tcgcgccggg gggtgtaggt ccccgttgtc
     7021 gggtcggtta gaatgaaagg cttcgtggat tcccggggct cggggggctc gaccctgatc
     7081 tatgggctcc cgcccggaga cgcccccccc cggccgtggg ggatacttct tccccccacg
     7141 gagccggcgg agacggaccg gggaaagaat tgttcccttt ccccggaaaa tggccggttt
     7201 tttgaataca aacacgggtt accattggag tgcaatccat gcaggtcgat gacatctctt
     7261 ctccgggaag caggacggat cgtctagggg tcctccgtca atcccgaaac gaggaaacat
     7321 acaccatgtc tccgaaccga tcccaactgg gcttgtccaa tctcaagccc ggagatcacc
     7381 tgtgttgggt ctacgaaacg gacgaagagc acagaagtgt cctcattccc tttctgtccc
     7441 agggagtgga gaacgacgag aagatcctct tcatctggga cgtgtcctcg gccgaagata
     7501 cggtgcgcta tttgcgaaac gactccatgg acgtggaagc ctgcctatgc cgggggcaac
     7561 tgagcatcat caccgccaaa gaagggtaca tgcggggggg atttttcgat cccgaggcca
     7621 tgatccgcct tctcgaaagg gagtcggcct tcgctcaggc ccaggggtac tcggtcctcc
     7681 gggtcgccat agcccctccc tccatgcccc atggtcttcc cgtccacaag cactttgcgg
     7741 atttcgagac gaagatgaac gtgttcatgc gccggcaatg ctgcctgatg ctctgcatgt
     7801 acgatcggcg ccgatattcc tcccagatgc ttctcgacat gttgaattcc caccccgtgg
     7861 tggtgttggg cacctctttc tacgaaaatt tctactatgt tcctcccaag gatctcctgg
     7921 gcgtggacgc ggccacggca gctttgaggg atcgtctgca gagcctcgcc ctacgaaaac
     7981 atgcggaaga gctgctgcgc atctccgagg ccaactatcg agccattttc aacgccgcca
     8041 acgacgccat tcttctcctg gacatggaga cgggggccat catcgacgcc aatgaaaaaa
     8101 cctacgagat gtgggggtac agtcccgaag agttcctgcg cctcaacgtc gaggacttga
     8161 tttccgaaga gcctccttac cagagggggg atggaattcg ccgcatcgaa aaggcggccc
     8221 acggcatccc ccagcttttc gaatgggtct tcaagaacaa ggtgggcaag agattctggg
     8281 gagaggtgaa tctcaagcgg gccttcaacg aggaaaagaa cttcgttctg gcggtggtga
     8341 gagacatcac cgagcgcaaa tgggccgaca agctgctccg ggagagcgaa gagcgttttc
     8401 gatccatctt tcacaacgtg tacgtgggcg tggccctggt ggacaaacag ggccacgttc
     8461 tggacgccaa cgaagccttt tgcgggttcc tgggatattc tccccgggag ctcatcggca
     8521 tgcatctaag ccggttcacg ttcgaagagg aactcaacga ggaaatgcag ctcctggacg
     8581 aactgtgcag cggcaagcgc cctacccacg ttatggacaa gcggtacctg cgcaaggacg
     8641 gggaggttgt ctggggacag ttgagcctct cggccatacg ggacaacgag ggaatgc
//
LOCUS       DAOOVC010000159         8627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5042_length_8627_cov_5.780331, whole genome shotgun sequence.
ACCESSION   DAOOVC010000159 DAOOVC010000000
VERSION     DAOOVC010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5042_length_8627_cov_5.780331"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..366)
                     /locus_tag="PLM79_15345"
     CDS             complement(10..366)
                     /locus_tag="PLM79_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87734.1"
                     /translation="MADARASCVRALLIQLIKYTEQLARCEDDPGVDVGVLADACYQR
                     LAMLQVLTREGLPADCRAEERAAEEHPTIRELMRTLRNRTRGCLGAMERSRDRIGKEL
                     DSDRQVRRAIQAYRGK"
     gene            complement(495..926)
                     /gene="fliS"
                     /locus_tag="PLM79_15350"
     CDS             complement(495..926)
                     /gene="fliS"
                     /locus_tag="PLM79_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOV87735.1"
                     /translation="MYYSDAVNSYQKTRVETVDDLKLVIMCYDATIRDLEDAKKLHQG
                     QAMEATYEKIRHAQDIITELLVGLDYDRGGVIAQNLSKLYNFLLRQLVGINSRKDTAM
                     YDHLIRILSELRSAWEQVRLNSLREVPVPTPAPARAWGVSA"
     gene            complement(989..3649)
                     /gene="fliD"
                     /locus_tag="PLM79_15355"
     CDS             complement(989..3649)
                     /gene="fliD"
                     /locus_tag="PLM79_15355"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOV87736.1"
                     /translation="MGISVSGLSSSFDWQNLVDQLRQVENSRLQTLSTQQSALKKKLS
                     AWSDFESLLSKLKSSAFALRNTDALNYFSASTTSSSSTVTADSLLSVTASTSAAKGTY
                     QVKVINKAQAEKLSSATFSSKTSALGMEGTFLVNGKAVNVTAGDTLENIRTKINQANT
                     GDEASGVVGSIIQESSNAYRLVLTSEVEGARGIGLVNGSSGDILSSLGFNSAGTTLKN
                     PVVGGASSDRFSSSSSSVEALLGIASHDLSGTVVINGTSVAVDLSDSLSTIKDNLNAA
                     FSAAGNRATARLVSETEGANTYYRLEIEGMTGWTDENNVLQALGFVEGKRENVVGVTG
                     GVANTTDGSTAITSSTRFEDIYGYLNPDPANDSITISGKKHDGTSVSTTFNLFNPDTS
                     SKTVGELLTEIQTAFGNVTASVTADGKIQVVDNEGGSSRLSVDLAASLHGTNPGSLSF
                     GTFTEVGTVRQYVLQEGRDASFTIDGVAMTSSSNTVTSAIPGVTLKLAGESADTTVTL
                     DIDLDYDKIKAQIQSFVDAYNEVVKFIKTQTSYNSETKTTGGVLFGDNQLKAMRTSIQ
                     STLIAEVFGTSNLKALSDIGITTDRSTALSLDTKVLQEKLETNFDDVVRLFARSGSSQ
                     NTSLEYFYSTRDTQAGTYAVHIDQAATKASIEGSGIDWGAGYTGSTGDTLTVKDAATG
                     KSITKSFTAGESLGDIVNTLNSLFDNAGIAVSASVNASNQLQLSQDNYGSGKTITLTY
                     EGGTKAALGLSIDSADGTNVAGTIGGVAAQGTGQILKITNTTSAAYGLQIRYTGTSTG
                     DVGDFTFTSGVANQLEKQLYQWMDSVSGSLTIHKNSIQSKIDRYDDRIASTEELIDTK
                     MAMLTKQFQNMESAISKLQSVQSYISSLLG"
     gene            complement(3828..4244)
                     /locus_tag="PLM79_15360"
     CDS             complement(3828..4244)
                     /locus_tag="PLM79_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87737.1"
                     /translation="MHEETLLDRLGRELIESWQVVAAGNGFLVTTDWRLPNDELIEIH
                     VRNVGDRKDLFVVTDGGEVVNVLFSHGFDLTGDRGAMRMAARSAERYGVKIVDGQMVK
                     GAGEEDLHRAVRKVLEAVKEVSCMLWHHLDPPDQVH"
     gene            4728..5498
                     /locus_tag="PLM79_15365"
     CDS             4728..5498
                     /locus_tag="PLM79_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87738.1"
                     /translation="MMKTYGKATAIVVLTFILSGPVLPAHAGEEAKEDKPTVNLSVDV
                     LSQYLWRGYALSSDSAVLQPSATVSYKGFSLNLWGNFDTDEALPNKGARWNETDFTFS
                     YARELFKGFNATVGGTYYSLVNSRYDSVEVFGGVSYAFPWVTVGVTGYREVSHSPGWW
                     VQMDLSKNFPLPCYGLSLDLGAGLGYLVLEDSNYSEFHAGQVFGALNIPLGKYFTVSP
                     RVGVSFPLSDGARREIETVSWDGEDTHVFGGIRLSAAF"
     gene            complement(5589..6038)
                     /locus_tag="PLM79_15370"
     CDS             complement(5589..6038)
                     /locus_tag="PLM79_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87739.1"
                     /translation="MKVFRLSPVLLLLVFLASCSLKPAWDLTGKWEKVDGKETLEFAQ
                     KGVVVLSSGSTTLTSTYKMADAKHLQIEVGSFGPLVFEIQLAGNDLVLKGTSGQELKF
                     KKVLPQPPAKAQAQAQAQPPAKAPAQPPAQAQPQGQGHAQASKPTAK"
     gene            complement(6054..6263)
                     /locus_tag="PLM79_15375"
     CDS             complement(6054..6263)
                     /locus_tag="PLM79_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87740.1"
                     /translation="MGKKVKIGFAGSRECLRALPADQVLRAFLPDSDPHRNPQRRLWA
                     WVVTLPVRGRGSNFVVEVYGIVNEF"
     gene            6774..>8627
                     /locus_tag="PLM79_15380"
     CDS             6774..>8627
                     /locus_tag="PLM79_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87741.1"
                     /translation="MRQYRRLTASILMSLVFLVGAVPPVSAQPKPSEGEGVLVGRISH
                     IDGGQILRFVPEEKDWVATVKDAPFGIDDALYSDPETKGEILMPNGTLLRIGGDTQVQ
                     MIALEDDVTEVDVASGLARLYNNSSRGLIKATTPFGYVLAPEKTTFDLYVGDESVEVI
                     GLRGRVDFVIESDGSRFEVLAGSSSLISDGRQVTSGKGDVDADWDDWNLDREGFWDRK
                     LQTRGESVRYLPEGLHREAYALEENGRWERVEYEGQARYFWRPVHVHADWAPFTVGRW
                     TVWQEDNCWVPDEPFGYVTHHYGNWVYVETARRWYWAPPVVRVAAVPLLNVGYGWYPG
                     RVAWIHSGAEVGWVPLAPFEPYYSHRWWGPRSMVVANVGLANIHINLGGYRYLNHAVV
                     VNQSNFWGVRSYTNVRIKNINKTVIINHYRPAPVVNQTVIKNYNVDRQRYHFTNVKVV
                     KKPHQTVVNRIEKNRNLAGRIGKVNAASLQKSTVNLKQGKLAPEAKIARPKVTNKLVS
                     ENEVNKPKGELKFNERELKKKERASREMQQERARGPEDRPRKPEVVKPDRRPPEDRPE
                     KAREEKGIQSPKELRSPQKEKVQPEQDRRRQREERPQVQEPRQREQQPPRED"
BASE COUNT         1684 a         2520 c         2657 g         1766 t
ORIGIN      
        1 tccccggagt tacttccccc ggtaggcctg gatcgctcgg cgaacctgtc ggtccgagtc
       61 cagctccttc ccgatgcggt cccgggatcg ctccatcgcg cccaggcacc cgcgggtccg
      121 gttccgaagg gtgcgcatga gttcccgaat ggtcgggtgc tcctccgcgg cgcgttcctc
      181 tgcccggcag tccgccggga gcccctcccg ggtaaggacc tgaagcattg ccagtctttg
      241 gtagcacgcg tcggcaagca cgccgacgtc caccccgggg tcgtcctcgc atcgggcaag
      301 ctgttccgtg tacttgatca actggatcag aagggcacgc acgcaggagg ctcgggcatc
      361 cgccatatac tttctcccta gggtttcctg gggaccgccg gtttgaaaaa gggtccttct
      421 tttctctctc cgtggatggg tgacgattgg ttctgctggt tctgcaggcc tcaaacgcga
      481 cagggcccca aagatcatgc ggaaaccccc caggcccttg caggggcagg ggtcggtacg
      541 ggtacctctc tcagagaatt gagacgcacc tgctcccaag cgctcctcaa ttccgagaga
      601 attcgaatga gatggtcata catggcggtg tctttgcggc tgttgatgcc gacgagttgt
      661 cgaaggagga agttgtagag cttgctgagg ttctgagcaa tgacgccccc tcggtcgtag
      721 tcgagaccga ccagcagttc ggtgatgatg tcctgggcgt ggcgaatctt ctcgtaggtg
      781 gcctccatgg cctggccctg gtgcagcttc ttggcatctt ccaggtcccg gatggtggcg
      841 tcgtagcaca tgatcaccag cttcaggtcg tccacggttt cgactcttgt cttctggtag
      901 ctgttcactg catcgctgta atacatgatt ccttacctcc ttcgtcaagg ccgccgttgc
      961 gcggcccggg tcgctacacc ctccctgact atcccagcaa gctgctgatg tagctctgaa
     1021 cggactgcag cttggatatg gccgactcca tattctgaaa ctgcttggtc agcatcgcca
     1081 tcttggtgtc gatgagctct tcggtggatg cgatgcgatc atcataccgg tcgatcttgg
     1141 actggatgct gttcttgtga atggtcaagg aacccgaaac ggaatccatc cactggtaga
     1201 gttgtttctc gagctggttt gccaccccgc tggtgaaggt gaagtcgccc acgtctcccg
     1261 tagaggtccc ggtgtagcgg atctggagac cgtaggccgc ggaggttgtg ttcgtaatct
     1321 tgaggatctg gccggtaccc tgggcggcaa cccccccgat ggtccccgcc acgttggtgc
     1381 cgtcggcgct gtctatggag aggcccaggg cggcttttgt tccgccttca taggtcaggg
     1441 taatggtctt gccgcttccg tagttgtcct ggctcaactg cagttggttc gaggcattga
     1501 cggaggcgct gacggcgatc cctgcattgt cgaacaggga attcaacgta ttgacgatgt
     1561 cgcccaggct ttcgcccgcg gtgaagctct tggtgatgga cttgcccgtg gcggcgtcct
     1621 tgacggtaag ggtatcgccg gtgctccccg tgtatcctgc accccaatcg atcccggagc
     1681 cctcgatgga agccttggtg gccgcctggt cgatgtggac ggcgtaggtg cccgcctggg
     1741 tgtcccgggt gctgtagaag tactcgagac tggtgttttg gctcgatccg cttcgggcga
     1801 agagtctcac cacgtcgtcg aaattggtct cgagcttctc ctgcaacacc ttggtgtcca
     1861 ggctgagagc ggtggatctg tccgtggtga ttccgatgtc gctgagggcc ttcaggttgg
     1921 aggtcccaaa cacctctgcg atcaacgtcg actgaatgct ggtccgcatc gccttgagct
     1981 gattgtctcc gaagagaacc cccccggtcg ttttggtttc gctgttgtag ctggtctggg
     2041 tcttgatgaa cttcaccacc tcattgtagg catccacaaa ggactggatc tgtgccttga
     2101 tcttgtcgta atccaggtcg atgtccaggg tcacggtggt gtcggcgctc tccccggcga
     2161 gtttcagggt tacgcccggg atggcggagg tgacggtgtt ggacgagctg gtcatggcta
     2221 caccgtcgat ggtgaaggat gcgtcccgac cctcctgaag cacatattgg cggacggtcc
     2281 ccacttccgt gaaggtgccg aagctcaagc tgcccgggtt ggtaccgtga agcgacgccg
     2341 ccaggtctac cgaaagccgg ctggacccgc cttcgttgtc caccacctgg atcttgccgt
     2401 cggccgtgac cgatgccgtc acgttgccga aagcagtctg gatttccgtg agcagctccc
     2461 ccaccgtctt cgaactggtg tcggggttga acaggttgaa agtggtggag accgaggtcc
     2521 cgtcgtgttt cttgccggag atggtgatgg aatcgttggc ggggtccggg ttcagatacc
     2581 cgtagatgtc ctcgaatcga gtgctgctcg taatggccgt ggagccgtcc gtggtgttgg
     2641 cgaccccgcc ggtaaccccc acgacgtttt ccctctttcc ttccacaaat cccaaggcct
     2701 gcaacacgtt gttctcatcc gtccacccgg tcatcccctc gatttccaac cggtaatacg
     2761 tattcgcccc ttcggtttcc gaaaccaggc gggccgtggc gcggtttcca gcggcgctga
     2821 aggccgcgtt gaggttgtcc ttgatggtgg aaagagagtc ggaaagatcg acggctacgg
     2881 acgtcccgtt gatgacaacc gtaccggaca aatcgtggga tgcgatgccc aggagagcct
     2941 ccaccgagga ggaagagctc gagaaccgat cgctggaggc accccccacc accgggttct
     3001 tgagggtcgt gcccgcactg ttgaagccca ggctcgagag aatgtctcca ctggatccgt
     3061 tgaccaggcc gattccccga gccccctcca cctcgctggt gagaacgaga cggtacgcgt
     3121 tggaagactc ctgaatgatg ctcccaacca cgccggaggc ctcgtcgccg gtgttcgcct
     3181 ggttgatctt ggttcgaatg ttctcgaggg tgtcgccggc cgtgacgttc accgccttgc
     3241 cgttgaccaa gaaggtgcct tccatgccca gggcggacgt cttggacgaa aaggtcgcgg
     3301 agctgagctt ttccgcctgg gccttgttga tcaccttcac ctgataggtg cctttggcgg
     3361 cactggtcga tgcggtgacg ctcaggagcg aatcggcggt gacggtggaa gaggaggagg
     3421 tggtcgaagc cgaaaagtaa ttgagggcat ccgtgttccg aagagcgaac gccgaggatt
     3481 tcagcttgga aagcagggac tcgaaatcgg accacgccga aagctttttc ttcagggcgc
     3541 tctgctgggt ggagagcgtc tgcaaccggg agttctccac ctggcgcaac tgatcgacca
     3601 ggttttgcca atcgaagctg ctcgaaagtc cactgacgct gattcccatc tctttctcct
     3661 gcgaagattg tcgatgcctt acgcagacta catcgtcgaa ttcgggagaa agctttaagg
     3721 ttttttggat ctcgaatgag gcgggctcag ggaagagggc gataccggaa ggaaaaacag
     3781 ggtcccgggg agacgggaat tctcgggggg ccgggacccc ccttcgttca atggacctgg
     3841 tccggggggt cgaggtggtg ccacagcatg caggagacct ccttcaccgc ttccaagacc
     3901 ttgcggacgg cccggtgcag gtcctcttcc cccgctccct tcaccatttg accgtccacg
     3961 atcttcactc cgtatcgttc ggccgaccga gcggccatcc tcatggcgcc gcgatccccg
     4021 gtcaggtcga agccgtggga aaacaggacg ttgaccacct cccccccgtc cgtaaccacg
     4081 aagaggtcct tgcggtctcc cacgttcctc acgtgaatct cgatgagttc gtcattgggc
     4141 aaacgccaat ccgtggtgac gagaaagccg ttccccgcgg cgacgacctg ccaggactcg
     4201 atgagctcac ggcccagcct gtccaacaac gtttcttcat gcatggtccc gatccttcct
     4261 ccctgggggg taggggtact ccggtctaag gtgtggggcc gttccccgcc acccgaggga
     4321 cgtgcccgtc ctcctgccgg caaccccggg gaaaataaca cccggcaggg aaaatgtcca
     4381 agggaacccc gggccgggat cgacccgaga atacggagac cggaaatgtg tgactagggt
     4441 cccataagag aggcaaaagg gacaaaattg tttcaaaagc tgttgttttt tctcatggta
     4501 ggctgggtat tttctccccg acaatcctcc tttttccctc cgtcgatcaa ggacgtaacc
     4561 ccttgcccga tgcgcaaata gagggacacc gctcctgccg gaccgggtcg gtttccgtct
     4621 tcatgaggcc ccctggccca atttgtgctc taggggggat accgggaggt accgggaacg
     4681 gtcgtctccg agcaagaatc cattcaagaa ggggaaaggg aaaatcgatg atgaaaactt
     4741 acggaaaagc cacagccatt gtcgtcctca cgttcatcct gtccggaccg gtcctcccgg
     4801 ctcatgccgg ggaagaggcc aaagaagaca aacccaccgt caacctttcc gtggacgtcc
     4861 tcagccagta cctctggcgc ggatacgccc tgagcagcga cagtgctgtc cttcagccgt
     4921 cggcaacggt ttcctacaag ggattttccc taaacctgtg gggcaacttc gatacggatg
     4981 aagccctccc caacaagggg gcgcgctgga acgaaacgga cttcaccttc tcgtacgccc
     5041 gggagctgtt caagggattc aatgcaaccg tgggcgggac ctattactcc ctggtgaatt
     5101 cccggtacga ttccgtcgaa gtctttggag gggtttccta cgcctttccc tgggtcacgg
     5161 tcggggtcac cggctatcgg gaggtatccc attccccggg atggtgggtg cagatggacc
     5221 tctcgaagaa cttccccctg ccctgttacg gcctgagcct ggatttggga gccggcctgg
     5281 gatacctggt cctggaagac tcgaactatt cggaattcca cgcaggccag gtctttgggg
     5341 ccctaaacat ccctctgggc aagtacttca ccgtgtctcc ccgggttggg gtgtcctttc
     5401 ccctttcgga cggtgcccgc agagagatcg aaacggtctc ctgggacgga gaggatactc
     5461 atgtcttcgg cgggattcgt ctcagtgcgg ccttctaagg tcccgaccgg tcggcatccc
     5521 ctcctcgaac ctcgatggtg ccaatgggag gggatgcagc ccggatcgat tcggcaaacg
     5581 ggttggaatc actttgcggt cggcttggac gcctgggcat gaccctggcc ctggggctga
     5641 gcttgagccg ggggttgggc cggggctttg gccggaggtt gtgcctgtgc ttgagcttgt
     5701 gctttggctg gaggttgagg cagaaccttc ttgaacttga gttcctggcc ggaggttccc
     5761 ttcaggacca ggtcgtttcc cgcgagctgg atttcgaaga ccagcggccc gaagctcccc
     5821 acctcgatct gcaggtgctt ggcatcggcc atcttgtacg tgctggtcag ggtcgtgctt
     5881 ccactcgaga gaacgaccac gcccttctgg gcaaattcaa gggtttcctt tccatcgacc
     5941 ttttcccatt tgccggtcaa atcccaggcg ggcttcaagg aacaggacgc gagaaaaacc
     6001 aaaagcaaaa gaaccgggga taaacgaaaa accttcatca agactcctcc tctctagaat
     6061 tcgttgacaa tcccatacac ctccaccaca aaattggacc ctctgcctcg caccggcaag
     6121 gtcacgaccc acgcccaaag cctccgttgc ggattacggt gcgggtcgga atccggaaga
     6181 aaggcccgga ggacctgatc cgccggcagg gcgcgcaggc actcccggga gcccgcgaag
     6241 cctatcttta ccttctttcc catgaatctg tccaccttca aatggaaaac cggcaccgcg
     6301 acctgcagtc aagcggcggg cttcccgacc ccgccaaggg gttgaggcat cctcggcacg
     6361 gaccgccggc ccgtgtcgac tttttcgggc tcgtcaccat ccatagggga tcgaaatgag
     6421 ccggggtccc aaacggtgca cataatggta actcgatgtg cagaaatggt actcgatgga
     6481 gaaggggcag ggtccaacaa taatccaagt acctgatgtt tcggtttttc ttctgtggaa
     6541 cggttattgc tcgtccggta gggcctcgga gtccccgcat catgggagcg gtgggacccg
     6601 aagcttctcc ccaaccgccg atcagagcgc aaaggagcca aggaatgtga gagaaggtcg
     6661 agaagagaga acggcttcgc cggttccgtc ccccccggga gcgcccggcg gaaaacttta
     6721 cgaaacgaag atcacagagg acgttttgag caatgacgaa gaggagtgac tcgatgaggc
     6781 aatatcgacg gctgaccgcg agtattttga tgagcctggt ctttctggtg ggggctgtgc
     6841 cgccggtttc cgcccagccc aaaccttccg agggggaagg ggtgctggtg gggcgcatct
     6901 cccatatcga cggggggcag atcctccggt ttgttcccga agagaaggac tgggtggcga
     6961 cggtaaaaga cgcacctttc gggatcgacg acgccctgta ttcggacccg gagaccaagg
     7021 gggagatcct catgcccaac ggtacccttc tgcggatcgg cggggatacc caggtccaga
     7081 tgatcgctct cgaggacgac gttacggagg tggatgtggc ttcggggctg gcccgcctgt
     7141 acaacaacag ctcccgggga ctgatcaagg ccacgactcc tttcggatat gtccttgccc
     7201 ctgaaaagac gacctttgac ctctacgtag gcgacgagtc ggtggaggtc atcgggctga
     7261 gaggccgggt ggactttgtc atcgaatccg acgggagcag atttgaagtc ctggcagggt
     7321 cttcctccct catctccgac ggcaggcagg tgacctcggg aaagggggac gtggacgccg
     7381 attgggacga ctggaacctc gaccgagagg gcttctggga caggaagctc caaacccggg
     7441 gagaatcggt ccggtacctc cccgagggac tccaccggga agcgtatgcc ctggaggaaa
     7501 acgggagatg ggagagggtc gagtacgagg gacaggcccg ctatttctgg cgaccggtcc
     7561 acgtgcatgc cgactgggct ccctttacgg tggggcgctg gaccgtgtgg caggaagaca
     7621 actgctgggt gcccgacgaa cccttcgggt acgtgacgca ccattacggc aactgggtat
     7681 acgtggaaac cgcccgcagg tggtactggg ccccccccgt ggtgagggtg gcggccgtgc
     7741 ccctcctgaa cgtcgggtat ggctggtacc cggggcgggt ggcgtggatc cactcgggtg
     7801 cggaggtcgg gtgggtcccc ctggccccct tcgaacccta ctactcccat cgctggtggg
     7861 ggccccggtc catggtggtg gccaacgtgg gcctcgccaa catccatatc aacttgggag
     7921 ggtatcgtta tctgaaccac gccgtggtgg tgaaccagtc caatttctgg ggcgtgcgca
     7981 gctacaccaa cgtgcgcatc aagaacatca ataagaccgt gatcatcaac cactaccgcc
     8041 ccgcccccgt ggtgaaccag accgtgatta agaactacaa cgtcgacagg cagaggtacc
     8101 atttcacgaa tgtgaaagtg gtgaagaagc ctcaccagac cgtggtgaat cgaatcgaga
     8161 agaatcggaa tctcgccggg cggataggaa aggtgaacgc ggcctctcta cagaagagca
     8221 ccgtcaacct caagcaggga aaactcgccc cggaggccaa gatcgctcga ccgaaggtga
     8281 cgaacaagct ggtttccgag aacgaagtga acaagcccaa gggggagctg aagttcaacg
     8341 aacgggagct caaaaagaag gaacgggcct cccgggagat gcagcaggag cgggcccgtg
     8401 gtcctgagga ccggcctcga aaaccggagg tggtcaagcc ggatcgaaga ccgcccgagg
     8461 atcgccccga gaaagcccgg gaggaaaagg gaatccaatc tcccaaagaa cttcgctccc
     8521 cgcagaaaga aaaggtccaa cccgagcagg atcgtcggcg acagcgggaa gaacgccccc
     8581 aggtgcagga gccacgacag cgggagcagc agcctccccg ggaagac
//
LOCUS       DAOOVC010000160         8614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5048_length_8614_cov_5.802080, whole genome shotgun sequence.
ACCESSION   DAOOVC010000160 DAOOVC010000000
VERSION     DAOOVC010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_8614_cov_5.802080"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLM79_15385"
     CDS             <1..198
                     /locus_tag="PLM79_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87742.1"
                     /translation="QDQLLIAEIDKLEENALPAIYVEISDGQDPDLKTDDLQEDKALF
                     HGVGEGIHTASRSQSERKRRS"
     gene            195..668
                     /locus_tag="PLM79_15390"
     CDS             195..668
                     /locus_tag="PLM79_15390"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HOV87743.1"
                     /translation="MRGKPIGFLVVLLGVMLPAVSFAQEIYPGKWWHVPPIREKLRLS
                     DKERRQLDELYDENRRRLVDLKEEAKREQSELRLLLETEPLKEQSVMAQARRLEEVRS
                     DLAVERLRYVLDVRKILGPDRFQQLKKMYRSSPEMRVPAYPGTPIPKKRPWYRFQ"
     gene            complement(730..1920)
                     /locus_tag="PLM79_15395"
     CDS             complement(730..1920)
                     /locus_tag="PLM79_15395"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOV87744.1"
                     /translation="MAKEDLRADVKKETDPVVREGADSRPSGEKRGGWAGGPAAIALW
                     AALFLLLGGVVGWSLARTLFAPQPTQIPTLHAHVESEWVPVRSPGAGTVRELKADVGH
                     PVEKGDVLVVVRDDGIEARTDKALADVRTLRQRLREAREKPAEVKRRSGPENPVARQA
                     LKSAGERLAVEEQRLRRIAKGTDSDYVLYESNPLPTEPSSSGMSARDMARRRVEEARA
                     EVEALRERREAGGTPEEDPGRLREWEEVLRSADREIEAIREALDAVRREYLLVGPVRG
                     LVMRSLVQVGETVRDGQVLLWVADLDGTWLEADLDPSDVGKVQVGQKVQGILEKPPGT
                     RVDGEIARVELIRGGDKGRAGDPDPSDPSASDPRYRIRVTPQNCPVELVPGMKASVRL
                     GPPR"
     gene            complement(2022..2849)
                     /locus_tag="PLM79_15400"
     CDS             complement(2022..2849)
                     /locus_tag="PLM79_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOV87745.1"
                     /translation="MEEKPKVVIIKKIKKDHGGHHGGSWKVAYADFVTAMMAFFLLLW
                     LLSMVSPEKRAVMALYFKYFSLFERGGKSFMQEGGVNPIIQSGGQEYYETGQEKNGGL
                     TELEEMTGKLMSEVQEQGESFKESIVVDTTELGVRIQIVDRAESPVFLPGSPQLTETG
                     KRLTKMVAGTMMNLPNEIVVEGHSDSSVGKNEPMNNWELSSLRACNAKKQLELDGIKP
                     DKISRVVGFADKSPLIKDNSLDPRNRRISIIFLYGKKQTPANPYGWVWQTPPASNPR"
     gene            complement(2849..3706)
                     /gene="motA"
                     /locus_tag="PLM79_15405"
     CDS             complement(2849..3706)
                     /gene="motA"
                     /locus_tag="PLM79_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOV87746.1"
                     /translation="MFVAIGMFIVLGAVVGGYLMEHGNLSVLYQPAEVVIIFGAALGG
                     FVTASPLKVIKATVGSIGRLFGGKVKTGDDYLEVLMLLNEIFFKIRKEGLVSIEADID
                     DPEKSEIFKKYPGFLKDHHSLAFLTDTLRTLTIMDIDAHELASLLDYEIEAHYEELLV
                     PSKSIATVADSLPGLGIVACVLGVVLTMGKIDSPPSVLGHYIGAALVGTFLGVLMCYG
                     FVGPVAKNLEHMASEDREYLNVLKVAILAFVNSPSPQIAVEFARRVIPADLKPSFLEL
                     ETVLKQAKK"
     gene            complement(3716..4228)
                     /locus_tag="PLM79_15410"
     CDS             complement(3716..4228)
                     /locus_tag="PLM79_15410"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOV87747.1"
                     /translation="MREYSRVDAFIPLKTRLTSLEERKDLHSRTSSECFSATFQPWPD
                     LQDSELAEALKILNAKLDAVLQYLTFGNGDPGTLQGSQVTISGGGMSFYSKEAYGVGE
                     FVEMQFMLPTVSNKVFFVYGEVIHVEEVCGERYRTGVQFTVIDEDIREEIVKFVFEKQ
                     RETLRKQRRL"
     gene            complement(4777..6222)
                     /locus_tag="PLM79_15415"
     CDS             complement(4777..6222)
                     /locus_tag="PLM79_15415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOV87748.1"
                     /translation="MFGDVEVRRVLSTGLLGGWIGLLLCGCMVGPEYRKPATQVPAAY
                     SELPSDRRAVAEPTNLAGWWNAFHDPVLTSLVDRAVQSNKDLKIALARVREARALRTI
                     VAADEYPAINASGSYTRTRQSENATSSASTSSMWQDLFQLGLDAGWEVDLFGGTRRSV
                     EAATADLEASEEGLRDVMVSLLAEIAVNYMELRGNQRRIAIARDNIEAQRQTVELTRA
                     KFQAGLSSELEIAQAEALLSTTESRVPQFESASKKAIHRLGVLLGAGPGALLQELSDP
                     SALPVAPPEIPVGLPSELLRRRPDVRRAERQLAAATARVGVATADLFPRFFLTGNAGW
                     QSVNASDLISPQSRALSFGPSIQWPVFDAGRIRANIRVQNARQEQALAQYEKTVLASL
                     EDVENSLVAYSKEQVTRNHLAQAVDANRRAVEISNELYTKGLVDFLNVLVNQRSLFQL
                     QEQLALSEQAVCTNLVALYKSLGGGWEDRLP"
     gene            complement(6307..6828)
                     /locus_tag="PLM79_15420"
     CDS             complement(6307..6828)
                     /locus_tag="PLM79_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOV87749.1"
                     /translation="MEKKVGEKTIRLIQGDLTELDVDAIVNAANTHLVLGGGVAGAVR
                     MKGGPTIQEECDRIGGTTVGQAVVTTGGNLKARYVIHAVGPRYGEGQEDFKLEQATWN
                     SLLRATEKGLGTIAFPAVSTGIFGFPKDRCARIMLDTAQRFLESEKTTVKEVIFCLWS
                     AEDLRIFEVALGE"
     gene            7358..7699
                     /locus_tag="PLM79_15425"
     CDS             7358..7699
                     /locus_tag="PLM79_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOV87750.1"
                     /translation="MKDKLRQALSAQRVAVVGASQEQLSVGIGPLHNLLSHSFRGEVF
                     PVNPKYDWLLGRKCYPTPEPIHPPPDLAILLLNQHSSLAMAERAALHGVKAVSNVAGG
                     FKEIRAEGAEL"
     gene            7757..8587
                     /locus_tag="PLM79_15430"
     CDS             7757..8587
                     /locus_tag="PLM79_15430"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87751.1"
                     /translation="MGSSNLGLGLHGIFRHLETFPGPTAIFTQSGGVGPTMGYALRSL
                     GTGLSYFIGVGNASVVDVPDYLEILEEDPTVRCFTLFLEGLQNPRLFFETARRVCIRK
                     PIVAYKAGKHEEVSKATQTHTGNLASDTRLYRAMMKQAGICEADSIRKAAAASKALGM
                     LPVPRGDRLCALTFTAGPPIVAMDKLLPAGWQFPDLSPDTLRKVRSIIGEATPVRLRN
                     PVDLTGPGFLPQTYAFGSSRPCSRTPPSMPISSFGTTTPSSGSPWWKWNRFCARPRGR
                     "
BASE COUNT         1638 a         2759 c         2448 g         1769 t
ORIGIN      
        1 caggatcagc ttctcattgc cgaaatcgac aagctcgaag aaaacgcctt gcccgccatt
       61 tatgtggaga tatcggacgg acaggacccc gatctgaaaa cggacgacct ccaggaggac
      121 aaggcccttt tccatggggt gggggaaggc atccacacgg cgagtcgatc tcagtcggag
      181 cggaagagac ggtcatgagg ggaaaaccca tcggatttct cgtggtattg ctgggcgtga
      241 tgctgcccgc tgtttctttt gcccaggaga tctatcccgg aaaatggtgg catgtgcctc
      301 ccattcggga aaagcttcga ctcagcgaca aagaacgacg ccagttggac gagctctacg
      361 acgaaaaccg ccgtcgactg gtggacttga aggaagaggc caaacgggag cagtcggagt
      421 tgagactttt gctcgagacg gaaccgctca aagaacagtc cgtgatggcc caggcccgca
      481 gacttgaaga ggttcggtcg gacctggcgg tggagcgact ccgatatgtc ctggatgtga
      541 ggaaaatcct ggggcccgac cgatttcaac agctcaaaaa gatgtaccgc agctctcccg
      601 aaatgagagt ccccgcctac ccgggtaccc ccatccccaa gaaacgaccc tggtatcgct
      661 tccaatgagc ccccgggaag gagccccctt gcctcggcga aagcacccgc ctcgcgtacc
      721 cgggacaagc taccgaggcg gccccaaccg gacggacgcc ttcatgccgg gcaccagctc
      781 gacgggacag ttctgcggag tcacccgaat tcgatatcgt ggatcggaag ccgaaggatc
      841 ggacggatcg gggtctccag cccggccttt gtcgccccct cgaatcagct ccacccgagc
      901 gatctccccg tccacacggg ttcccggagg cttctcgagg attccctgca ccttctgtcc
      961 cacctggacc tttcccacat ccgaagggtc caaatcggcc tcgagccagg tcccgtccag
     1021 atcggcgacc cacaagagga cctggccgtc ccgaacggtt tctccaacct gcacgaggct
     1081 ccgcatcacc agcccccgca cggggccgac caacaagtat tcccgcctta cggcatcgag
     1141 cgcttcccgg atcgcctcaa tctcccggtc ggcagaccgg aggacctcct cccactcccg
     1201 caaccgcccg ggatcttcct ccggggtccc tccggcctcc cggcgctcac ggagggcctc
     1261 gacctcggct ctagcctctt ccacccgtct tcgcgccatg tcccgggcgc tcatgccgct
     1321 cgaggagggt tccgtcggca aagggttgct ttcgtacaaa acatagtcgg agtccgttcc
     1381 cttagcgatg cgtcgaagtc gctgttcctc gacggccagg cgttccccgg ccgatttcag
     1441 ggcctgtcga gcaacagggt tctccggtcc gctccttctc ttgacttccg ccggtttctc
     1501 ccgggcttcg cggagccttt gccggagagt acgaacgtcg gcaagggcct tgtccgtccg
     1561 agcctcgatt ccatcatccc gcaccacaac gagaacgtcg cccttttcca cgggatgccc
     1621 cacatccgcc ttcagttctc gaacggttcc ggcgccggga cttcggacgg gaacccattc
     1681 ggactccaca tgggcatgga gggtcggtat ttgggtcggt tggggggcga agagcgttct
     1741 tgcaagggac catccgacga cgccccccag gagcagaaat agggcggccc acagggctat
     1801 ggccgcaggc cccccggccc aaccccctct tttttctccc gatggacggg aatcggcccc
     1861 ttcccggacc acggggtccg tctccttctt cacatcggcc ctgagatctt ctttggccat
     1921 ggggtgctcc tggtatgccg gggaagcaaa gtccctcaat gagcggggtg ggcgtccccg
     1981 tcggggaggg accccacggt cggtcctcaa ggttcttcga gtcagcgagg attggacgcg
     2041 ggaggggtct gccagaccca tccgtaagga ttggctgggg tctgcttctt accgtaaaga
     2101 aagatgatgc tgatcctgcg gtttcgagga tccagcgaat tgtccttgat cagggggctc
     2161 ttgtccgcaa atcccaccac tcgactgatc ttgtcgggct tgatgccgtc cagttcgagc
     2221 tgttttttgg cattacacgc ccgcaggctg gacaattccc aattgttcat gggttcattc
     2281 ttccccacgg aggaatccga gtgaccctcc accacgattt cgttgggcag attcatcatg
     2341 gtgcctgcaa ccattttggt gagcctcttg ccggtctccg tgagttgagg acttccgggt
     2401 agaaacacgg gggactccgc ccggtccaca atctggattc gtacccccag ttccgtggtg
     2461 tccaccacga tgctttcttt gaagctctcc ccctgctctt ggacttccga catcaatttg
     2521 cccgtcattt cctccagttc cgtcaggccg ccgttcttct cttgccccgt ctcgtagtac
     2581 tcctgtcccc cggactggat aatggggttc actcctcctt cctgcatgaa ggattttcct
     2641 ccgcgctcga aaaggctgaa gtatttgaaa tagagggcca tcacggcgcg cttctccgga
     2701 gagaccatgg acagcagcca caacagcagg aagaaggcca tcatggcggt gacaaagtcg
     2761 gcgtacgcca ccttccagct tccgccgtga tgccccccat gatccttctt gatcttcttg
     2821 atgatgacga ctttgggctt ttcttccact atttcttcgc ctgcttgagg accgtttcga
     2881 gctcgaggaa gctgggcttc agatcggcgg gaatgaccct tcgggcaaat tccaccgcga
     2941 tctgaggcga cggactgttc acaaaggcaa gaatggccac cttcaacacg ttgagatact
     3001 cccggtcctc actcgccatg tgttccaggt tcttggcaac gggacccaca aagccgtagc
     3061 acatcaacac acccaggaag gtccccacca gggccgcacc gatgtagtgc cccagcacgg
     3121 aagggggtga atcgatcttt cccatggtga gcaccacgcc caagacacag gccacaatcc
     3181 ccaagccggg cagagaatcc gccacggtgg cgatggactt ggatggaaca agcagctcct
     3241 cgtagtgggc ttcgatttcg taatccagca aggatgccag ttcatgagcg tcaatgtcca
     3301 taatggtcaa ggttcgcagc gtatccgtaa ggaacgcaag ggaatgatga tccttgagaa
     3361 accccggata tttcttgaag atctcgctct tttccgggtc gtcgatgtcc gcttcgatgg
     3421 acaccaggcc ttctttgcga atcttgaaga agatctcgtt gagaagcatc aggacctcga
     3481 ggtaatcgtc cccggtcttc accttgcctc caaacagtct gccgatgctt ccaacggttg
     3541 ccttgatcac cttgaggggg gaagccgtga cgaagccgcc caaagccgcg ccaaagatga
     3601 tgactacctc ggcgggctga tacagcacgc tcagattgcc gtgttccatc aggtagccgc
     3661 cgaccacggc tcccagaacg atgaacattc caatggccac aaacatggtt tcagcctaaa
     3721 gcctccgctg tttcctcagg gtttccctct gcttctcgaa aacgaacttc acgatttcct
     3781 ctcggatgtc ttcgtcgatg accgtgaact gcacgccggt tctgtaacgt tcgccacaca
     3841 cctcttccac gtggatcact tccccgtaga cgaaaaacac cttgttggaa acggtgggga
     3901 gcatgaactg catctcgacg aactccccca cgccgtaggc ttccttggaa tagaagctca
     3961 ttcctccccc gctgatggtg acctgactgc cctggagtgt tccggggtcg ccgttgccga
     4021 aggtgagata ctggagaacc gcatccaact tggcgttcag gattttgagc gcctcggcca
     4081 gttcactgtc ctgtagatcg ggccacggct gaaacgttgc cgagaagcac tccgacgagg
     4141 tgcgggagtg aaggtctttc ctttcttcca acgacgtgag ccgtgttttg agggggatga
     4201 acgcatcgac ccgcgagtat tccctcatgt tccgtctgct ctcttggtcc ataatcaacc
     4261 tgccggttcg cccccgaaac ggggttgttc ccatggtgag acgggtctct gtacccgggg
     4321 acaacatccc gtgggcgttt ctgagttttt catgcgttct gcgcgctaag aatgccgtcc
     4381 cgtcctcttt gcccgatgat ccgaagattc cctggggcaa cggcgtctcc cgagctgcag
     4441 tcgcgtggtt tcccggccct gtgcttagtt agctattcgg gtttcggcgg acaaaccttt
     4501 agggaaatcc gagaccgggt gggtgggatc gaggtgaacc acatccgtgg cggccccccc
     4561 tggatccggt ttcggagagg gcgcccccgg ggggaaagca aacggtcccg ggaatcggta
     4621 cggaagattg cggaagcccc cgatggtcag gacggactcg gcccggaccc ggaaaacggc
     4681 atagacccgg atatcggccg aaaggaaggc ccccatgggg ttcgacggcc tgcaatccga
     4741 agcattgctc cgtttggggc cggacctccc cgctcttcac gggagccggt cctcccatcc
     4801 gccccccaga gacttgtaga gggccacgag gttcgtacag accgcctgct cgctgagggc
     4861 cagctgttcc tggagctgga agagacttcg ctgattgacc agcacattga ggaaatccac
     4921 cagacccttg gtgtagagct cgttggagat ttccacggcc cgccggttgg cgtccacggc
     4981 ctgggcgagg tgattgcggg tcacctgctc cttggaataa gccaccaggg aattttccac
     5041 atcctcgagg gacgcgagaa cggtcttttc gtattgggcg agggcctgtt cctggcgtgc
     5101 attctgaacc cggatgttgg cacgaatcct tcccgcatcg aagaccggcc actggatgga
     5161 aggaccgaag ctgagggctc gactttgggg cgagatcagg tccgaagcgt tgacactttg
     5221 ccagcctgca tttcccgtga ggaaaaaccg cgggaagagg tccgccgtgg cgacccccac
     5281 cctggcggtg gccgcagcca gttgtctttc ggcccggcgc acgtcgggac ggcggcgcag
     5341 cagctccgac ggcagcccta cggggatttc ggggggagca acggggagtg ccgacgggtc
     5401 cgacaactcc tggagcagcg ccccgggccc cgctcccagg aggactccca gccgatggat
     5461 ggctttcttg gatgcggact cgaactgggg cacccgggac tcggtagtgg acagcagcgc
     5521 ctcagcctgg gcgatttcca gctcactgct gagcccggcc tggaacttgg ctcgagtcag
     5581 ctccaccgtt tgtcgctgag cctcgatgtt gtcccgagcg atggcgatgc ggcgctgatt
     5641 tccccgcagt tccatgtaat tgaccgcaat ctccgccagc aacgagacca tcacgtcccg
     5701 aagaccctct tccgaggcct cgaggtccgc cgtggccgcc tctaccgacc tgcgcgtccc
     5761 tccaaaaagg tccacctccc aaccggcatc caggcccaac tgaaagaggt cctgccacat
     5821 ggacgaggtg gaagcggagg aggtagcgtt ttcgctctgg cgagtgcggg tatagctccc
     5881 ggaagcgttg atcgccggat actcgtccgc ggccactatc gtgcgcaagg cacgggcttc
     5941 ccgcactcgc gccagggcga tcttcaggtc cttgttggac tgcacggctc gatccacgag
     6001 ggaggtgagc acggggtcat ggaaagcatt ccaccacccc gccaggttcg tcggttccgc
     6061 aacggccctg cggtccgaag gcaattcgct ataggcggca gggacctgcg tggcgggttt
     6121 tctgtattcc ggaccgacca tgcacccgca caaaaggagg ccgatccatc ctcccagaag
     6181 ccccgtggaa aggaccctcc tcacctcaac atccccaaac attcccacac tccctgttga
     6241 cacgtcgttc accgtcgagc tcgaggattc attccacgca ggtccggccc cggaggaccc
     6301 ctccccctat tctcccaggg cgacctcaaa gattctcaga tcttccgccg accacaggca
     6361 gaaaatcact tccttgacgg tcgtcttttc cgattccagg aatcgttggg cggtatccag
     6421 catgatccgg gcgcatcgat ccttgggaaa gccgaagatc ccggtgctga cggcaggaaa
     6481 tgcgatagtg cccaagcctt tctccgtagc gcgaagaagg ctgttccagg tggcttgctc
     6541 caacttgaag tcttcctgtc cttctccgta tcgcggtccc accgcgtgga tcacatatcg
     6601 agctttgagg tttccaccgg tcgtcaccac cgcctgtcct acggtggtgc ccccgatacg
     6661 gtcgcattcc tcctgaatgg tcggtccccc cttcatgcgg acggcaccgg ccaccccccc
     6721 gcctaaaacc aggtgggtat tggcggcgtt gacaatagca tccacgtcca gttccgtcaa
     6781 atcaccctgg atcaggcgaa tggttttctc tccgaccttc ttttccatga cacaccctcc
     6841 tgtgcggttg gcggaaatgc tggttctccc cggttgggtg tgattctacg acgacttgtt
     6901 ccggggccca agagaataag gggttcgctc cggtgatttg cccgaacgcg gtgccggctc
     6961 cccaaccccc tgatacgaag tcgcactcaa gccgagactg cccacccgtt ggattcagcc
     7021 ctttgctcga aaccttcact cagcatgtca gggcgacctt gggcgcaact tggtatgagg
     7081 agtcccaggg ccgggttgtg gggcgtccct gtccagtccg tagaagggat tgcacaaggg
     7141 accgaccgtc gggagagaac ggctcccttt cagcttcatc gaacgcggga ccaggccgga
     7201 gttgatttat ccccggactg tactaacctc tgcgcggctg cgggacccgg tggccgcccg
     7261 gagtcgtttt ccggttcctt tcttgtttgg aaggatgtgc aggctcttcg acccactcgc
     7321 ccacgaaccc ggagttcttc atgccccttt gctcaacatg aaagataagc tgcggcaggc
     7381 gctcagcgcc caacgagtgg ccgttgtcgg agcttcccag gaacaactgt ccgtcggaat
     7441 cggccctctt cacaatcttc tttctcattc cttccgcggg gaggtttttc ccgtaaaccc
     7501 caagtacgat tggcttttgg gacggaaatg ctacccgacc ccggaaccca tccatccccc
     7561 tccggacctg gcaatcttgc ttctcaacca acattcctcc ctggccatgg cggaacgggc
     7621 ggctctccac ggcgtcaagg cggtatccaa cgtggcgggg ggcttcaaag aaatccgggc
     7681 cgaaggggcg gaactgtaag agaaactccg ggagctggcc tttcgacacc agttgccggt
     7741 aattggaccc aacaccttgg gctcttcaaa cctgggtctc gggttgcacg gaatcttccg
     7801 gcacctggag acgttccccg ggccgacggc catctttacc caaagcggcg gggtcggtcc
     7861 gacgatgggg tatgccctgc gaagcctggg caccggtctc agttacttca tcggggtggg
     7921 caatgcttcg gtcgtggacg ttccggatta cctggaaatc ctggaggagg acccaaccgt
     7981 tcgttgcttt accctgtttc tggaaggact gcagaatccg cggctctttt tcgaaaccgc
     8041 ccgcagggtc tgcatccgca agcccatcgt cgcctacaag gcaggcaagc acgaggaggt
     8101 gtccaaagcc acccagaccc acaccgggaa tcttgccagc gacacccggc tctatcgcgc
     8161 catgatgaaa caggccggga tttgtgaagc cgacagcatc cggaaggctg cggcggcatc
     8221 caaggccctg ggcatgctcc ccgttccccg gggcgatcgt ctctgcgccc tcaccttcac
     8281 cgccggtccc cccatcgtgg ccatggataa gttgctcccg gcgggatggc agtttccgga
     8341 cctctccccc gatacccttc ggaaggtccg gtccatcatc ggcgaggcaa cccccgtccg
     8401 acttcggaat cccgtggacc tgacgggccc gggttttctc ccccagactt acgcattcgg
     8461 gtcctcaaga ccctgctcga ggacccctcc ttcgatgcct atttcatcgt ttggaactac
     8521 aaccccctca tccgggtccc cgtggtggaa atggaatcgc ttctgcgcgc gacctcgagg
     8581 ccggtagtgg cggccttccc ggccaaccgg aggc
//
LOCUS       DAOOVC010000161         8614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5049_length_8614_cov_5.302021, whole genome shotgun sequence.
ACCESSION   DAOOVC010000161 DAOOVC010000000
VERSION     DAOOVC010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5049_length_8614_cov_5.302021"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2578
                     /locus_tag="PLM79_15435"
     CDS             <1..2578
                     /locus_tag="PLM79_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV87752.1"
                     /translation="DALTQDVLLAPIESSVIPLPHQIRALSRAISNDRVRYLLADEVG
                     LGKTIEAGLIMRELKLRGLVRRTLVIAPKGLVTQWVAEMRTHFGEEFRLLIPSDFSAY
                     RRIAQEDNLWQSHPQVVCPMDSVKPLDGRRGWSREQVAEYNRERFEDLISAGWDLVIV
                     DEAHRLGGSTDQVARYKLGQGLAEAAPYLLLLSATPHQGKTDHYRRLMSLLDAEAFPD
                     EASITKDRVQPYVIRTEKRRAIDAEGKPLFKPRRTELAPVSWKERHRDQRLLYEAVTE
                     YVREGYNQAITEKRSYIGFLMILMQRLVVSSTRAIRTTLERRLEALEAPQEQLTLFPM
                     VSEEDWADLDGQEQMDTLLKTRLKALKNERAEVKLLLEAAKRCESQGPDAKAEALLDW
                     IYRLQAEEGDPDLKVLVFTEFVPTQEMLYEFLTERGFEVVCLNGSMDLEERKRVQDAF
                     AKDARILISTDAGGEGLNLQFCHVVINYDIPWNPMRLEQRIGRVDRIGQTHTVRAINF
                     VFEDSVEHRVREVLEEKLAVIFEEFGIDKTGDVLDSAQAGQIFDDLYVEAILNPDVVE
                     TKVDEVLKSIQEQARESRQSASVLGSTEDLDPREAQRLLAHPLPHWVERMTVSYLQAH
                     GGKAERKNGAWDLTWPNGERLTNVVFTSKEAEEKPLSRHLTLEDPKVRGLAMRLPPFA
                     PGQPVPVITLPGLAPEIMGFWSLWRISISTVDWNRRRIMPLFLADDGRVFAPTARHIW
                     DQLVTTPPTILRHLDIETSHQAFDQLRGAAERQGKTIYDELVQAHRERLAREREKGEY
                     AFAARRRAIERIGLPQVRNHRLSLLEQEEERFREQLERRAQILPEMAPLLLVRVEGCA
                     HG"
     gene            2571..4577
                     /gene="pglZ"
                     /locus_tag="PLM79_15440"
     CDS             2571..4577
                     /gene="pglZ"
                     /locus_tag="PLM79_15440"
                     /inference="COORDINATES: protein motif:HMM:NF033449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HOV87753.1"
                     /translation="MGSWRDQILSEFTPQVARLTLVADPDGLLLEEGVLEGIRERGFE
                     LIPFEDHVAFRYAYESKFRSRWDRGEKTDLVVVLRSPSSDLDALPYDLLQAGRKLSFN
                     LGDLFPNLSYPVVAALDRADLDALFDAQTKHAPGQLGDNATKEFVLRHVFEIAPELIK
                     QPSDLLRVLLRRHYRGQRIPAILDERFIQVLRQNGLFEDWPLETIIPDAQAFFAFLQE
                     RWPVFLDSLAKPKDDAVHEDAADSGFEFPGPTLLPFDHHDVRIFIDNLFLEGLLQPVP
                     HEQSQALAKTWVAYGIKVSPVENRRRRVEGLLESIEKTIPTVETRHGEWLHFAYRWAE
                     LVALELEPDTTLPEGYRERLDALRSRIDSALTGWVVKRYASLINLPPAPPVMLHHIPR
                     FLARSLGDDRRSKIAFLLVDGLALDQWIALREVLGELDSKLRFRESAVFAWIPTITSV
                     SRQAAFAGKPPIYFPTSIHTTDKEPGLWTQFWVDQGLNANEVAYAKGLGEGDLDKVSE
                     LLSRPRLRVVGLVIDKVDRIMHGMELGAAGMHNQVRQWARQGFMRDLFGLLYDRGFQV
                     YLASDHGNIEAKGAGRPAEGAVADLRGERVRVYSDPRLRAQIKERFPESLEWPSVGLP
                     EDYLALIAPNRAAFVRAGETLVGHGGISVEELLVPLVQIDRRDR"
     gene            4574..5356
                     /locus_tag="PLM79_15445"
     CDS             4574..5356
                     /locus_tag="PLM79_15445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87754.1"
                     /translation="MNSRLTQIGFSQRVRLEWLERTANLVLAGNDEASIYNALQELFE
                     DKLSVGGNAKRGNREKVITILMKIWVRPPRDLHPLQREGLKLLSHLPREDHIAVHWGM
                     AMAVYPFWGAVAAHVGRLLRLQGTAAASQVQRRVREQYGERGTVSRAARRVLRSFVDW
                     EVLKETSEKGIYTAGLSLAIAQVEVIAWLAEAFLHAHPNASVALRTVLDSTSLFPFRL
                     SPVSAAHLVAVSGRLDVLRHGLDQDLIMLREESLPAAKGGGS"
     gene            5404..6198
                     /locus_tag="PLM79_15450"
     CDS             5404..6198
                     /locus_tag="PLM79_15450"
                     /inference="COORDINATES: protein motif:HMM:NF020452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HOV87755.1"
                     /translation="MKPHMEGRATETSSSGDFFDEWLEVRDWTIRRHRKILLREKDGA
                     RDAILDDLRNVVRSHYVSPETMAARIEQLGADKTAELLREHLPTKKGSRSGDLGEILA
                     TEFVTRKLAYEVPILRLRWKDGREMALRGDDVVAFRRDEDDRLSFLKGEAKSRARLTP
                     DIVQQAANALDQDHGRPGRHSVLFVAERLREQGADEMAVLLENAVLNSFSSCEVEHLL
                     FTFSGNSPDAHLSNHLEALRSGRRRYAVGLRIDDHCDFIQAIYEGL"
     gene            6535..>8614
                     /locus_tag="PLM79_15455"
     CDS             6535..>8614
                     /locus_tag="PLM79_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV87756.1"
                     /translation="MAFHLARYAARAYSILPDGTIETNTSFSELALARLLRRSLDELH
                     EQLSTWLLDEVHRDAAVAERLQTDDDYDEYDAIDDILTTSLMRGLALFDHALRTGTTA
                     SANRAKQLLLTGAAAAADLHVVPHWWILTLAAHLIDELWDHSLHERLPKLPPDHPDER
                     WNGLRRDYIHRLRRNSRAAIELWPSQITAAERAIDPADDLIIALPTSAGKTRIAELCI
                     LRALAAGKRVVYVTPLRALSAQVERDLSDTFVPLGFPVSSLYGSAGVEAADADTLRTK
                     EIVVSTPEKLDFALRNDPTIIDDVGLIVLDEGHMLGPGEREVRYEALVQRLLRRDDAG
                     DRRIVCLSALFPETEKMTDLVAWIRRDRPGEPVHSEWRPTRQRFGWIQWEGRAARLQV
                     RVEEEESYVPRFVEAKPPPSGSRRRNTFPQNKNELTLATAWQFAGHGKDVMIYCARRD
                     SVESLGKLILKCGRQGVLSRLGDVTPRIREVMATGKEWLGPNHPAVRCLEYGVALHHG
                     GLPRQFLSEVERLLRSGDCRVTIASPTLAQGLNLSASVLLVPSIWRTQGEKISPVEFA
                     NVAGRAGRAFVDLEGLVLHVVWERNQRKRRWVIQQWEELVAQARASEIESGLLRLTAL
                     LFERIARAADVSIEEVIEYVTGHGEAWDYTELVGERTEVSESEWERDAASLDAAILGL
                     LDVETAIDEIE"
BASE COUNT         1681 a         2601 c         2689 g         1643 t
ORIGIN      
        1 cgatgcgctg acccaggacg ttctccttgc gcccatcgaa tcgtccgtca tcccgttgcc
       61 gcatcagatt cgggctctct ccagggccat ttccaatgac cgcgtgcgct atctcctggc
      121 ggacgaggtg ggcctcggca agaccatcga ggcaggtctc atcatgcggg agctcaagct
      181 ccggggcctg gtgaggcgaa cgctggtgat cgccccgaag ggcctggtga cccaatgggt
      241 agcggagatg cgcacccact tcggggagga gttccgcctg ctcatcccga gcgacttctc
      301 cgcctaccgc cgcatcgccc aggaagacaa cctctggcag agccatccac aggtggtctg
      361 ccccatggat tcggtgaagc ccctggacgg ccggcgcggc tggtccaggg aacaggtggc
      421 cgagtacaac cgggagcgct tcgaggacct gatctccgcc ggctgggacc tggtgatcgt
      481 ggacgaggcc catcgcctgg gtgggagcac ggaccaggtg gcccgctaca agctcggcca
      541 ggggctggcc gaggcagcgc cctacctgct cctgctctcc gccacgccgc atcagggcaa
      601 gaccgaccat taccggcggt tgatgtcact ccttgatgcg gaggcgtttc ccgacgaagc
      661 gagcatcact aaggaccggg tacagcccta tgtcatccgc acggagaagc gccgcgccat
      721 cgatgcggaa ggcaagccgc ttttcaaacc ccggcgtacg gagctggcac cggtctcctg
      781 gaaggagcgc caccgggatc aacggctgct ctacgaggcg gtcaccgaat acgtgcgaga
      841 aggctacaac caggccatca ctgagaagcg cagctacatc ggattcctca tgatcctgat
      901 gcagcgcctg gtggtctcca gcacccgcgc tatccgaacc accctggagc ggcgcctcga
      961 agcgctcgag gcgccccaag aacagctcac actcttcccc atggtctccg aggaggactg
     1021 ggccgacctg gacggccagg agcagatgga caccctgctc aaaacacgtc tcaaggccct
     1081 gaagaatgaa cgggccgagg tgaagctgct cctggaggcg gccaagcgct gcgaaagcca
     1141 gggcccggac gccaaggctg aagcgctcct ggactggatc taccgcctcc aggcggagga
     1201 aggcgacccg gatctcaaag tgctggtgtt caccgagttc gtgccgaccc aggagatgct
     1261 gtacgagttc ctgaccgagc gtggcttcga ggtggtgtgc ctcaacggct ccatggacct
     1321 ggaggagcgc aagcgggtcc aggacgcatt cgccaaggat gcccgcatcc tcatctcgac
     1381 ggacgccggc ggtgaggggc tcaacctcca gttctgtcat gtggtcatca actacgacat
     1441 cccctggaac ccgatgcgtc tggagcagcg catcggccgg gtggaccgca tcggccagac
     1501 ccatacggtg cgcgccatca acttcgtgtt cgaggattcg gtcgagcacc gtgtccgcga
     1561 ggtcctggag gagaaattgg cggtcatctt cgaagagttc ggcatcgaca agaccggtga
     1621 cgtgctcgac tctgcccagg cgggccagat cttcgatgat ctgtatgtgg aggccatcct
     1681 caatcccgat gtggtggaga cgaaggtcga cgaggtcctg aaaagcatcc aggagcaggc
     1741 ccgggagtcc cggcagagcg cttccgtgct cggctcgacc gaggacctgg accctcgcga
     1801 ggcccagcgg ctgctggccc acccgctccc gcattgggtg gagcgcatga cggtaagcta
     1861 cctgcaggct cacggcggca aggctgagag gaagaacggc gcctgggatc tcacctggcc
     1921 caacggcgag cgactgacca atgtcgtctt caccagcaag gaggctgaag agaagccttt
     1981 gagccgccac ttgacgctgg aagatcccaa ggtgcgtggg ctggccatgc gcctacctcc
     2041 ctttgccccg ggacaaccgg tccctgtcat cacgcttcct ggccttgccc cagagatcat
     2101 gggtttctgg tcgctctggc gaatctcgat ctcgaccgtc gactggaatc gtcgccggat
     2161 catgcccttg ttcctggccg acgacggtcg ggtgttcgcg ccgaccgccc ggcacatctg
     2221 ggatcaactg gtgaccactc cgcccacgat cctccggcat ttggacatcg agacctctca
     2281 tcaagccttc gatcaactcc ggggggcggc ggaacgacag gggaagacga tctacgacga
     2341 gctggtgcag gctcaccggg agcgtcttgc tcgtgagcgt gagaagggcg agtacgcctt
     2401 cgctgcgcga cgcagagcca tcgagcgcat cggactgccc caggtccgta accatcgcct
     2461 gagtctcttg gaacaggagg aagaacgctt cagggaacaa ctcgagcgca gggcccagat
     2521 cttgccggaa atggcgcccc tgcttctcgt ccgcgtggag ggatgcgccc atgggtagtt
     2581 ggcgtgacca aatcctgagt gagttcacac cccaggtcgc caggcttacc ctggtcgcgg
     2641 atccggatgg cctgctcctt gaagaaggtg tcctcgaagg gatccgggaa cggggctttg
     2701 agctgatccc gttcgaggat catgtcgcct ttcgctatgc ctacgaatcc aagttccgct
     2761 cccgctggga ccgtggcgag aaaaccgatc tggtggtggt gctgagatcg ccgtcgagcg
     2821 atctcgatgc cttgccctac gacctgctgc aggctggccg caagctctcg ttcaacctgg
     2881 gcgatctctt cccgaatctc agctaccccg tggtcgcggc gctcgaccgg gctgatctcg
     2941 acgccttgtt cgatgcgcag acgaagcacg cgccggggca gctcggcgac aacgctacga
     3001 aggagttcgt ccttcgtcat gtgttcgaga tcgcgccgga actcatcaag cagccatcgg
     3061 acctgctccg ggtcttgctg cgccgccatt accgcggaca acgaatcccg gccatcctcg
     3121 atgagcgctt tatccaggtg cttcgtcaaa acggcctgtt cgaggattgg cctctggaga
     3181 ccatcattcc ggacgcgcaa gcattctttg cgttcctgca ggagcgctgg ccggtgttcc
     3241 tggattcgct cgcaaagccg aaggacgatg ccgtccacga ggacgcggcg gactccggct
     3301 tcgagttccc aggaccgacg cttttgcctt tcgaccacca cgatgtccgc attttcatcg
     3361 acaacctatt cctcgagggt ttgctacagc cggttcctca cgagcaatcg caagcgctcg
     3421 ccaagacctg ggtggcttac ggcatcaaag tctcccccgt ggagaaccgt cgccgccgcg
     3481 tggaggggct gctggaatcc atcgagaaga cgatccccac ggtggagacg cgtcatgggg
     3541 agtggctcca ttttgcctac cgttgggcgg aactcgtcgc cttggagctc gagccggaca
     3601 ccaccctgcc ggaaggatac cgggagcgcc tagatgcatt gagatcccgg atcgattctg
     3661 cgctcaccgg ttgggtggtg aagcgctacg cgagcctgat caaccttccg cccgcgccgc
     3721 cggtgatgct gcatcacatc ccgcggttcc tcgcccgaag cctcggagac gaccggcgct
     3781 cgaagatcgc ttttctgctg gtggacggcc tcgccctgga ccaatggatc gccttgcgcg
     3841 aggtgctcgg agagctggat tcgaagctgc ggttccgtga gagcgccgtt ttcgcctgga
     3901 ttcccaccat cacctcggtt tcccggcagg ccgcctttgc cggcaagcca ccgatctact
     3961 tcccaacgag tatccatacc acagacaagg agccgggcct ttggacgcag ttctgggtcg
     4021 atcaggggct caacgcaaat gaggtggctt acgccaaggg gctgggcgag ggggacctgg
     4081 acaaggtctc cgagctactg agccggccca ggctacgtgt cgtcggattg gtcatcgaca
     4141 aggtcgaccg gatcatgcac ggcatggagc tgggtgcggc gggcatgcac aaccaggttc
     4201 ggcaatgggc caggcagggt ttcatgcggg accttttcgg cctgctttat gaccgcggtt
     4261 tccaggtcta ccttgcttcg gaccacggaa acatcgaggc gaaaggcgca ggaaggcctg
     4321 ccgaaggggc ggttgcggac ctgcgcggcg agcgcgtgcg tgtctactcc gatcccaggc
     4381 tcagggctca aatcaaggag cgattcccgg agtccttgga gtggccctcg gtgggcctcc
     4441 cagaggacta cctcgccctc atcgctccga accgggctgc ttttgtgcgg gcgggtgaaa
     4501 ccctcgtcgg tcatggaggc attagtgtcg aggaactgct cgtccctctt gtccagatcg
     4561 ataggaggga ccgatgaaca gtcgcctgac ccagatcggt ttcagccagc gggtccgtct
     4621 tgaatggctg gagagaacgg ccaacctggt gcttgccgga aacgacgagg cgtccatcta
     4681 caacgccctc caagagcttt tcgaagacaa gctgtctgtc ggcgggaatg ccaaacgcgg
     4741 caatcgggag aaggtgatca ccatcctgat gaagatctgg gtccgccccc cgcgcgacct
     4801 ccatcccctt cagcgggaag gcctcaagct tctctcacac ctgccgcgtg aggaccatat
     4861 cgcggtccac tggggcatgg ccatggcggt ttatccgttc tggggggccg tagcggccca
     4921 tgtggggcga ctgctcaggc tccagggaac ggccgcagcc agccaggtac agcgccgcgt
     4981 gcgggagcaa tatggggaac gcggcacggt atccagagcg gcacgacggg tgctgcggag
     5041 cttcgtcgat tgggaggtcc tgaaggaaac atcggaaaaa ggcatctata ccgctgggct
     5101 ttcactcgcc atagctcagg tggaggttat cgcctggttg gccgaggcct tcttgcacgc
     5161 tcatcccaac gcttcggtgg ctttgagaac ggtcctcgat tccacgagcc tgttcccgtt
     5221 ccgcctgagc cctgtttccg cggctcatct ggtcgcggtc tccgggcggc tcgatgtgct
     5281 gcgacacggc cttgaccagg acttgatcat gctccgcgag gaaagcctgc cagcggcgaa
     5341 gggcggcgga tcatgacgaa agagatagca caggcctgaa gtggatcggg gcgaatgaat
     5401 ggtatgaagc cacatatgga aggtcgggcc accgagacat cttcctcggg agatttcttc
     5461 gacgagtggc ttgaggtccg cgactggacc attcggcgtc accggaagat cctgcttcgc
     5521 gagaaggatg gagcaaggga tgcgatcctc gatgacttgc gaaatgtagt acgaagtcat
     5581 tacgtctccc ctgagacgat ggcagcaagg atcgagcaac tgggcgcaga caaaacggct
     5641 gagctgcttc gggaacatct tcccaccaag aagggctccc gttccgggga cctcggtgag
     5701 atccttgcaa cggaattcgt cactcgaaag ctcgcctacg aagtgcccat cctacggctc
     5761 cggtggaagg acggcagaga gatggcgctg cggggggatg acgtggttgc cttccgcagg
     5821 gatgaggacg atagactctc ttttctcaaa ggggaagcca agagccgcgc gaggctgact
     5881 cccgatatcg tccaacaggc tgcaaacgcg ctcgaccagg accatggacg accgggtcgt
     5941 cactccgtcc ttttcgtcgc ggagaggctt cgagaacagg gtgcggacga aatggccgtt
     6001 ctactcgaga acgccgtttt gaacagcttc agtagttgtg aagtcgagca ccttctgttc
     6061 acgttctccg gcaattcgcc tgatgcccac ttgtccaacc acctcgaggc gctccgaagc
     6121 ggaagacggc gttacgcagt gggtctgcga atcgatgacc actgtgattt catccaagcg
     6181 atctacgagg gtctgtagat gccagagccg gttgcaatac tcgaatcccg aatccagcga
     6241 gcgatcgccc caggtttccg cgatcggctc ttggacaagg gattggctcg cgggctcatc
     6301 tggcgtgatg gtgctcttcc tccagggtcc cctcgcttcc ccaggacact gacggaagac
     6361 ctgctcgatt tcggacacac cgtccttgcg atggcgctcc ggctgcgagg tgtttccccg
     6421 gacagtcccc atctcaaacg agctttccta tgcgcaggcg aagccattga gagtgccgtt
     6481 caccaagggg agccaggacg gactgattcc ggctttcatc gggtgaccgc cgcagtggcc
     6541 tttcaccttg ctcgatatgc ggctcgcgcc tattcgattc tgccggatgg taccatcgaa
     6601 acaaacacat cattctccga gctggctctc gcgcgattgc tgcggcgctc gctggacgag
     6661 ctccacgaac agctctcgac atggttgctg gacgaggtac atcgtgatgc tgcggtagca
     6721 gaacgtctgc agaccgacga cgactacgat gagtacgatg cgatcgacga catcctgacg
     6781 acgtcactca tgcgtggcct cgcgttgttc gatcacgcat tgcgtaccgg aaccacggca
     6841 tctgcaaatc gagccaagca attactactg acgggggcgg cggccgccgc cgacctccat
     6901 gtggtccctc actggtggat cctcaccctt gctgcccatc tcatcgacga gctctgggat
     6961 cacagcctcc atgaacgtct cccgaagctg cctcctgatc atccggatga acgctggaac
     7021 ggactgcgga gagactacat tcatcgcctc cgcagaaaca gccgagctgc aatcgaactc
     7081 tggccatcac agatcacggc agccgagcgc gccattgatc ccgcagacga cctgatcatc
     7141 gccttgccga ccagcgctgg gaagacgcga attgcggagc tctgtatcct cagggctctg
     7201 gcggctggca aacgtgtcgt gtacgtgact ccattgcgag cgctgtccgc tcaagtcgaa
     7261 cgggacctct ccgatacctt cgttcctctg gggttccccg tatcttcctt gtacggatcg
     7321 gccggggtcg aagccgctga cgcagacacg ctacgtacga aggagatcgt cgtatcgaca
     7381 cccgagaaac tggacttcgc tcttcgaaac gacccgacga tcatcgacga cgtcggtttg
     7441 atcgtcctcg acgaagggca catgctcggg cccggggagc gggaggtgcg gtacgaggcc
     7501 ctggtgcagc gtctcctccg gcgagatgac gcaggtgatc gacgtatcgt gtgcctgtcg
     7561 gcattgttcc cagagacgga gaagatgacc gacttggtgg catggattcg acgtgatagg
     7621 cccggagaac ccgtacattc cgaatggcga ccgacccgac agcgattcgg atggatccaa
     7681 tgggagggtc gcgctgcgcg gcttcaggtc cgagtcgagg aagaggagtc gtatgtcccg
     7741 cgattcgttg aagccaaacc tcccccgtca ggttcgagga ggaggaatac ctttccccag
     7801 aacaagaacg aattgacact tgcgacggca tggcagtttg ctggccatgg taaggacgtc
     7861 atgatctatt gtgctcgcag agactcggtg gagtctctcg gaaaactcat tctcaagtgt
     7921 ggacgtcaag gagtgctttc gcggctcggg gatgtcacgc cgcgtatccg tgaggtgatg
     7981 gccactggaa aagagtggct tgggccgaat catccggcag ttcggtgctt ggaatacggt
     8041 gtcgcactcc accacggagg tttgcctcgt cagttcctct cggaggtcga acggctgcta
     8101 cggtcgggag attgtcgtgt gaccatcgca tcaccgaccc ttgctcaagg actcaatctg
     8161 tctgcaagcg tgcttctcgt tccatcgata tggcgtaccc agggagagaa gatctcacca
     8221 gtggagttcg caaacgtggc cggccgtgcg ggacgggcgt tcgtggactt ggaaggattg
     8281 gtgcttcatg tcgtatggga gaggaatcaa aggaagagaa ggtgggtgat ccaacaatgg
     8341 gaagaactcg tagcacaggc gagggcttcc gagatcgaaa gcgggttact ccgtcttaca
     8401 gctttgcttt tcgagcgaat cgcaagagct gcagatgtct cgattgagga ggtcatcgaa
     8461 tacgtaactg gccacggaga agcgtgggat tacacggaac tcgtgggtga gcgcaccgaa
     8521 gtctccgagt ccgaatggga acgtgatgcc gcgtcgctgg atgcggcaat attgggtctc
     8581 ctcgatgtcg agacagccat tgatgaaatc gagc
//
LOCUS       DAOOVC010000162         8420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5176_length_8420_cov_6.438016, whole genome shotgun sequence.
ACCESSION   DAOOVC010000162 DAOOVC010000000
VERSION     DAOOVC010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5176_length_8420_cov_6.438016"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1853
                     /locus_tag="PLM79_15460"
     CDS             60..1853
                     /locus_tag="PLM79_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87757.1"
                     /translation="MEKGQVDIQRALELVLPYFRRHALQVCGGFLTLVAVDFLQLLIP
                     RVIKAVVDGLRAGTATPSRLALYAGLILLIAVFIAALRYFWRRLILGFSRLVELNLRN
                     RMVSHILTLDRAFFQRKTAGEIMALATNDLSSVQLATGMGLVAAVDAGFMGLAAIAFM
                     AYIHPGLTALAVAPMPILAVLTRLLSRRLHRRFKKVQEQFSHLTEFARSTFSSIRLIK
                     AYNQEVSQGKRFEALGEAYVGDNVKLAVIYGTLFPISGFIGNLGLLLVLLYGGKMTIG
                     GELTAGDFVAFVNYLFLMTWPMMALGWVSDLFQRGITSLDRIRNLLDEQPRLQDPVPD
                     APLPRIQGRITLKGLSFTYPGQREPALEDIHLDLPPGSFWGIVGRTGAGKTTLCHLLD
                     RLYPVQDGTYFLDGRDVNRLPLSAVRKAVAYVPQDVVLFSDTIARNLCLGKPEAGREE
                     MIRAARAAAIHDEILAMKDGYETLIGEKGIKLSGGQRQRLAIARALLLDRPILLIDDG
                     LSAVDVDTEQTILRSIGTHLRGRTCIVVSHRIAPLEEAQGILVMEEGRIVDRGTHAEL
                     LQRSRYYGILYGQQGNGDPGRRDRNSLSLTS"
     gene            1874..3622
                     /locus_tag="PLM79_15465"
     CDS             1874..3622
                     /locus_tag="PLM79_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87758.1"
                     /translation="MHNPYDSMDDEAAGHLGDLSLWKRLLGFVRPQRNRVAGAVLLSL
                     IITATGLLLPYLIQLALDGYILKEDLDSPARMQGVARLSLVFVVVVLVGFTANFFQVT
                     VLERAGQEMMHSLRRLLFTHVLNLNVSFFHGQRVGRLTTRLTNDIQNLHEMFTSVIVT
                     LFNEGVRLVGIIAILLRLNWRLALLLSLILPVMLWITAWFGRLSRQAFREIRKHLASI
                     NAFIQEMVTGISVLQLFQREEDTHRRFVGLNEEYYGSALFQIRVFGVFVPLIEVMNSL
                     SLVLIIGYGGMEILGGRMTIGVLAAFIAYMRLFFQPLRELSQKYSVVQSAMASAERIF
                     QLLDTRDFQPASSDAVRLEKVRGIIEFKDVVFEYEPGRPVIHRVSFRIEPGKTLAVVG
                     ATGSGKTTLIHLLERFYDPIEGRIELDGTDLRRLDVTWLRGQIGLVMQDVFIIPGSVR
                     ENILLDGDASEEEIERAVDLAQLGPLMKRLPHGLDTCIGEGGTDLSAGQRQLLSFARV
                     LLRNPRILVLDEATASVDPETEMFVEQAVRAVLMGRTNIVIAHRLSTIQRADQILVMD
                     RGRIVERREWGANLPP"
     gene            complement(3665..4828)
                     /locus_tag="PLM79_15470"
     CDS             complement(3665..4828)
                     /locus_tag="PLM79_15470"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87759.1"
                     /translation="MNLLLPQTIQTLAERTPREGSPVLSVYLHLDPSHPLNQKGGYKL
                     VLDGFLKDIEAQIREETWLRHFEEDAEWVRRKADHYLPKGKSLVLFCDVSESFFFEEA
                     LPIRMGNQAWCGSTPYIRPLLEAIEEYERYGVVLLDRERARFFIITMGSIEEISDIFQ
                     DPPVKHRSMAGSDHMRSQMVFQRRAAKWSESFLKYASDIVNDMVLHYGIDRILLAGPE
                     DVTAELLRMFPKRVASRVVHRMRMAVQAKPAEVMEISFPAIEQIENRQEREAVEDLVT
                     VARKANPTKEKAVLGINGILDAVNQGRVQRLFYPRGVKIPGSQCASCEVLLDHAPTDG
                     KCPYCSSSLTEVEDLLWPASERVLATGGKIQEIRGKEAHEHLRNAGEVGAYLR"
     gene            complement(4946..5644)
                     /locus_tag="PLM79_15475"
     CDS             complement(4946..5644)
                     /locus_tag="PLM79_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOV87760.1"
                     /translation="MTGTVINVGALLVGSLAGVFLGSRLSEPMRRTVLQCLGLITLVV
                     GIQMALETRNILIVMGAMLLGGIAGELLRIHDGLERLGSLLQSLLPPGKRGRVAEGFV
                     TASLVFCVGPMAILGSFQDGISGDFRLLSVKSVLDGFASVAFSAGLGWGVPFAGLTVL
                     LYQGGLTLFAGLFSAILTEPMIHEMTAAGGLIIVGIGIKLLELKEVRLASFLPALAVA
                     PLIVFLIPKVQALF"
     gene            6061..6255
                     /locus_tag="PLM79_15480"
     CDS             6061..6255
                     /locus_tag="PLM79_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87761.1"
                     /translation="MAVWKCNNCGNTVDLASPPEKCPSCKEKCEFVDVSCYIPECGGP
                     GTGHVNPQVFQESYKSDKRK"
     gene            6603..7616
                     /gene="aroF"
                     /locus_tag="PLM79_15485"
     CDS             6603..7616
                     /gene="aroF"
                     /locus_tag="PLM79_15485"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOV87762.1"
                     /translation="MIIVLKPDHQEEDLHRIIRRLEDLGLRTHISKGEQRTIVGAIGD
                     ERLLREVPLESLPGVESVLPILKPYKLVSREFKPENTVIQVDGREIGGNQLQVIAGPC
                     AVESREVLEEIAEELVPLGVNFLRGGAFKPRTSPYAFQGWGEKALKYLAGVRERTGML
                     VVTELMDPRDTVLVCKYADVVQIGTRNMQNFRLLTEVGNIDKPVILKRGMSATIKEFL
                     MSAEYILAQGNEKVILCERGIRTFETETRNTLDISAVPLLKDLTHLPVIVDPSHAVGR
                     ADLVPAMGKAAVAAGADGLLVEVHVRPEEAMSDGPQSLRPRVFRQMLDDCRKIARAVG
                     RDL"
     gene            7639..>8420
                     /locus_tag="PLM79_15490"
     CDS             7639..>8420
                     /locus_tag="PLM79_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOV87763.1"
                     /translation="MREDTELPGFYRSTDDPAPRLLTAESRIGVIGGAGEMGRLFSRF
                     FRERGLHVEVADREASRNNREVVAQSDVVVFAVPLHKTVEIIRDLVPYTRSDQLLMDV
                     SSLKTAPIREMLASSASVVGLHPMFGGRISSFEGQTLVACPVRISPRNWAEVRGMFTR
                     AGIRVKESDPEEHDRMMSIIQVLFHMTTMLKGRVLRELGVDIEDTLEYTSPSYRLEIN
                     LLGRMFAQNGSLYSAIAQMNPYTRDILAHLHQGLDAYERWYG"
BASE COUNT         1663 a         2522 c         2568 g         1667 t
ORIGIN      
        1 tcccttcgag acttgccccg ggagcaggaa atcccgtgaa gcgtcttttc tccccgggga
       61 tggaaaaggg gcaggtggac atccagcggg ccctcgagct ggtccttccc tacttccggc
      121 gccatgccct gcaagtttgc gggggattcc tgaccctggt ggcggtggat ttcctccagt
      181 tgctgattcc ccgagtgatc aaagcggtgg tggacggcct ccgggcggga accgccaccc
      241 cgtcccgtct ggctctctac gccgggctga tcctgctcat tgccgtgttc atcgccgctc
      301 tgcgctattt ctggaggaga ctcatcctgg gcttctcccg gctggtggaa ctgaacctca
      361 ggaaccgcat ggtctcccac atcctcaccc tggatcgggc gtttttccag cgcaagaccg
      421 ccggggaaat catggccctt gccaccaacg acctctcgtc ggttcagttg gccaccggga
      481 tgggattggt tgccgccgtg gatgccgggt tcatgggcct ggcggccatt gccttcatgg
      541 cctacatcca tccgggactc acggccctgg cggtggctcc catgcctatc ctggccgttc
      601 tcacccgcct tctgtcccgg cgtcttcacc gccgattcaa gaaggtccag gagcagttct
      661 cccacctcac ggagttcgcc cggtccacct tttcctcgat ccgtctcatc aaggcctaca
      721 accaggaggt ctcccagggg aaacggttcg aggccctggg ggaagcctac gtgggggaca
      781 acgtcaagct ggcggtgatc tacggcaccc tttttcccat ctccgggttc atcggcaacc
      841 tgggactgct cctggtcctc ctctacggag ggaagatgac catcggggga gagctcaccg
      901 caggggattt cgtggccttt gtgaactatc tcttcctcat gacctggccc atgatggccc
      961 tggggtgggt ctcggatctc ttccagcgag gaatcacctc cctggatcgc attcggaacc
     1021 tcctggacga acagccccga ctgcaggacc cggttccgga tgctccattg ccccgcatcc
     1081 aggggcgcat caccctcaaa ggcctcagct tcacctatcc ggggcaaaga gaaccggccc
     1141 tcgaggacat tcacctggac cttccgcccg ggtctttctg ggggatcgtc gggagaacgg
     1201 gggcgggcaa gaccaccctg tgtcacctcc tcgaccgcct ctacccggtc caggacggga
     1261 cctacttcct ggacggccgg gacgtgaacc ggctgcctct ctcggcggtg cgaaaggccg
     1321 tcgcttatgt cccccaggac gtggtgctct tttcggacac cattgcccgg aacctctgcc
     1381 tgggaaagcc cgaagccggg cgggaggaaa tgatccgggc ggcccgggcc gcggcaatcc
     1441 acgacgagat cctggccatg aaggacgggt acgaaaccct catcggagaa aagggaatca
     1501 agctttccgg ggggcagcgt cagcggctgg ccatcgcccg agccctgctc ctcgatcggc
     1561 ccattctcct tatcgacgac ggcctgtccg ccgtggacgt ggatacggaa cagaccattc
     1621 tgcgctccat cgggacccac cttcgagggc gcacctgtat cgtggtgtcc caccgcatcg
     1681 cccccctcga ggaagctcaa ggaatcctgg tgatggagga aggcaggatc gtggaccggg
     1741 gaacccatgc cgagctgctc caacgcagcc gctattacgg catcctctat ggacaacagg
     1801 ggaacggaga cccggggcga cgggacagga attccctctc cttgacttcc tgaagacaca
     1861 gagcacacgg accatgcaca atccctacga ttccatggat gacgaagcgg cgggccattt
     1921 gggcgacctg agcctgtgga agaggctgct ggggtttgtg cggccccagc ggaaccgggt
     1981 ggcgggggcc gtcctcctgt ccttgatcat tacggcaacg gggctcctcc tgccgtacct
     2041 catccagttg gccctggacg gctacattct gaaggaggat ttggattcac ccgctcgcat
     2101 gcagggggtt gcccggctct ccctggtctt tgtcgtggtg gtcctcgtgg ggttcacggc
     2161 caatttcttc caggtgaccg tactcgagcg ggccgggcag gaaatgatgc attccctccg
     2221 ccggctccta ttcacccatg tgctgaacct caacgtgtcc ttttttcacg gacagagagt
     2281 gggccgactg accactcggc tcaccaacga catccagaac cttcacgaga tgttcacctc
     2341 cgtcatcgtg accctcttca acgagggagt tcgcctggtg gggatcatcg ccattctgct
     2401 ccggctcaac tggcgcctgg ccctgctcct gagcctcatc ctgccggtga tgctctggat
     2461 caccgcctgg ttcggacgac tcagccgcca ggccttccgg gaaatccgca aacacttggc
     2521 ctccatcaac gcattcatcc aggagatggt caccgggatt tcggtgctgc agctgtttca
     2581 gagggaagaa gacacgcacc ggaggtttgt gggcctgaac gaggagtatt acgggtcggc
     2641 cctctttcag atccgggttt tcggggtttt cgtccccctc atcgaggtca tgaactcctt
     2701 gtccctggtc ctgatcatcg ggtatggggg catggagatt cttggaggcc ggatgaccat
     2761 cggagtgctg gccgccttca ttgcctacat gaggctgttc tttcagcccc tgagggagct
     2821 ctcccagaag tattccgtgg tccagtccgc catggcttcg gccgagagga tctttcagct
     2881 gctcgatacc cgggatttcc agcctgcgtc ctccgatgcg gtgcggctcg agaaggtccg
     2941 gggaataatc gagttcaaag atgtggtctt cgagtacgaa cccggtcggc cggtcattca
     3001 ccgggtttct ttccgaatcg agccgggaaa gacccttgcc gtcgtcgggg cgacgggatc
     3061 ggggaagacc accctcatcc atctcctcga aagattctac gatcccatcg aggggagaat
     3121 cgaactggac gggacggact tgagacgcct ggacgtgacg tggttgaggg gccaaatcgg
     3181 cctggtcatg caggacgtct tcatcatccc gggatccgtt cgggaaaaca tcctgttgga
     3241 cggggatgcc tccgaggagg agatcgagag ggccgtcgac ctggcgcagt tgggccccct
     3301 gatgaaacgg ctgccccacg gcctcgatac ctgcatcggg gaaggcggaa cggatctttc
     3361 ggcgggacag aggcagctgt tgtcctttgc acgggtattg ttgcgaaacc cgcgaatcct
     3421 ggtcctggat gaggccaccg ccagcgtgga cccggaaacg gagatgttcg tggaacaagc
     3481 cgtccgggcc gtgctcatgg gccggaccaa catcgtgatc gctcaccggc tgtccaccat
     3541 ccagcgggcg gaccagatcc tggtgatgga ccggggaaga atcgtggaac gcagggaatg
     3601 gggggcaaat cttcccccct gacggtcccg cggctcctcg attcgtcccg gtcccgatgg
     3661 tgccctaccg cagataggct cccacttctc cagcgtttcg caggtgctcg tgcgcttcct
     3721 ttccccggat ttcctggatc ttgcctcccg tggccaggac ccgttccgag gcgggccaaa
     3781 ggaggtcctc gacttccgtt agggaactcg agcagtaggg gcatttgccg tcggtgggag
     3841 cgtggtccag gagaacctcg caggatgcac actgggatcc cgggatcttc acgcctcgag
     3901 ggtagaaaag ccgctgaacc ctgccctggt tgacggcgtc caggatcccg ttgattccca
     3961 ggacggcctt ttccttggtg ggattggcct tcctggcgac ggtgacgagg tcctccacgg
     4021 cttcccgttc ctgccgattt tcaatctgtt cgatggcggg aaaggagatc tccatgactt
     4081 cggcgggttt tgcctggacg gccatcctca ttcgatgaac cacccgggaa gcaacccgct
     4141 tgggaaacat gcgcagaagc tccgcggtga cgtcctcggg ccccgccaag agaatccggt
     4201 cgattccgta gtggaggacc atgtcgttca cgatgtccga ggcatatttc aggaaggact
     4261 cgctccactt ggcggctcgc cgctggaaca ccatctggga gcgcatgtga tcgctccccg
     4321 ccatgcttcg gtgcttgacg ggggggtctt gaaagatgtc cgaaatttcc tcgatggagc
     4381 ccatggtgat gatgaaaaac cgtgctcgtt ctcgatccag caggaccact ccgtagcgtt
     4441 cgtattcctc gatggcttcg aggagcgggc gaatgtaggg ggtcgagccg caccacgcct
     4501 ggttgcccat tcgaatgggc agggcttctt caaaaaagaa ggactcggaa acgtcgcaaa
     4561 agagaaccag gctttttcct ttgggtaggt agtggtccgc cttccggcga acccactccg
     4621 cgtcctcctc gaagtgacgc agccacgttt cttcccggat ctgcgcctcg atgtccttga
     4681 gaaacccgtc caggaccagc ttgtaccctc ccttttgatt gaggggatga gatgggtcca
     4741 agtggaggta gacgctcagc acgggagatc cttcccgggg agtgcgttcc gccaaagtct
     4801 gaatcgtttg gggaagaagc agattcatcg gtctgacctc cttcttgggt tatatggggt
     4861 ccacgtcgat cttgctccaa agacaaaccc gccgggagaa gcagagaaaa gatccgcaga
     4921 ggcaaggtcc gaaacggggg ctttcctaga aaagcgcctg caccttgggg atcaggaaca
     4981 cgatgagggg tgcaacggcc agggcgggta ggaagctggc caaccgaacc tccttgagct
     5041 ccagtagctt gattccgatg cccacaatga tgagacctcc cgcggcggtc atctcgtgaa
     5101 tcatgggctc ggtgagaatg gcggaaaaaa gaccggcaaa cagagtcaat cctccctggt
     5161 acaggaggac ggtcaggccg gcaaagggaa ctccccaccc cagaccggcc gaaaaagcca
     5221 ccgaggcgaa cccgtccagg acggacttga cggacaggag tcggaaatct cccgaaatcc
     5281 cgtcctggaa ggatcccagg atcgccatgg gacccacgca gaagaccagg cttgccgtca
     5341 cgaacccctc agccacccgc ccccgttttc ccgggggcag gagggattgc agaaggctgc
     5401 ccaacctttc cagcccgtcg tgaattcgaa gcagctctcc tgcgatgcct cccaggagca
     5461 tcgctcccat cacgatcagg atattccggg tctccaaagc catttggatg cccaccacga
     5521 gggtgatcag gcccaggcat tggaggaccg tccgacgcat aggctccgag agccgggaac
     5581 cgaggaagac tcctgccagg ctccccacga ggagggctcc cacgttgatc accgtgccgg
     5641 tcaaccgttg cgctccttgc agttttgggg tatggggtcg ggcccggccc cggcgggcct
     5701 cgataggccc aaaaccggcg gtttccaggc aagcaaccga ctccgtaacc taagataatc
     5761 aaagcggttt tcgttgggaa gcaaaatgtc cgccccaacg gcgacctccg ggtgttcggg
     5821 ggaattcccg gccataccct cgcattggtt ccgaaaggaa tacgggaact acaggagggg
     5881 cggttgattt ttcaccctcc gtgcgttaat agtcagcata catttcttgg ttgctgaagg
     5941 gggttccggt ttgtccggtt gtttgagcgg agagccgctt ccgggaaaag gaaacacgtt
     6001 ttcggcgctc ccgagggtga cgaagggatc ataaacccat caactggaaa ggagcaagaa
     6061 atggccgtct ggaaatgcaa taattgtgga aatacggtgg atctcgcgtc tccgcccgaa
     6121 aaatgtccgt cctgtaagga gaaatgcgaa ttcgtggatg tatcctgcta cattcccgag
     6181 tgcggagggc cggggaccgg acatgtgaat ccccaagtgt tccaggagag ctacaagtcc
     6241 gacaagagaa agtaagggac gcacggggct cgacccgctg ttgaccaggt tcgaagaaag
     6301 tgcttgacag gccccggtgc ttttctctaa ggtgcgggca cgaaacgaac cgtcaaggaa
     6361 agcgtgaaat gcatacccga agcgaattct tttattattg gtattatttt accgggcctc
     6421 ccaggggagt ttggggtatg caccgctctt agcgcttgac gattgactgg aaacacaaaa
     6481 gccgtgggta tctcgaacat acctgcggct tttttgtttt ggcgcactcg caaccctccc
     6541 gccggagggg ccgctcccgg ctccccgtgg gacaaacaga ggaagaagga ggaacataag
     6601 ccatgattat cgtcttgaaa ccggatcatc aggaagaaga tctccaccgc atcattcgac
     6661 gcctcgagga cctgggcctc aggactcaca tctccaaggg agaacaacgg actatcgtgg
     6721 gggccatcgg ggacgagcgg ctcctgcggg aagtgcccct cgagtccctg ccgggtgtgg
     6781 aatccgtgct gccgattctg aaaccctaca agctggtaag cagggagttc aaaccggaga
     6841 acacggtcat ccaggtggac ggaagagaaa tcggcggcaa tcaactgcag gtgattgccg
     6901 ggccctgtgc cgtggaaagc cgcgaagtac tcgaagaaat tgccgaggaa ctggttccct
     6961 tgggagtgaa cttcctgcgg ggcggagcct tcaagccgcg gacctctccc tatgccttcc
     7021 agggatgggg agagaaggcc ctgaaatacc tggcaggggt tcgggaacgg acgggaatgc
     7081 tcgtggtgac ggaattgatg gatccccggg acaccgtgct ggtctgcaag tatgcggacg
     7141 tcgtccagat tggaactcgg aatatgcaaa acttccggct gcttacggaa gtgggcaaca
     7201 tcgacaagcc cgtgatcctc aagcggggca tgagcgccac catcaaggaa tttctcatgt
     7261 ccgccgaata catccttgcc cagggaaacg agaaggtgat tctttgtgag cggggtatcc
     7321 gcacttttga gacggagaca aggaacaccc tggacatcag tgccgtcccc cttctcaagg
     7381 acctgactca tttgcccgtc atcgtggatc cgagtcatgc ggtggggcga gcggacctgg
     7441 tgcccgccat gggcaaggcg gccgttgccg ccggggccga cggtctcctg gtggaagtcc
     7501 acgtgcgtcc tgaggaagcc atgagcgacg gcccccagtc tcttcgtcct cgcgtcttca
     7561 ggcaaatgtt ggacgactgt cgaaagattg ctcgagccgt aggccgcgac ctatgaatcc
     7621 cggacaggag ggattgacat gagagaagac acggaactgc ccggctttta tcgctccacg
     7681 gacgatccgg ccccccggct cttgactgcc gagtcccgca tcggagtcat cgggggggcg
     7741 ggagaaatgg ggagactgtt ctcgaggttt tttcgggagc gaggattgca tgtggaggtg
     7801 gccgaccggg aggcttcccg aaacaaccgg gaggtcgtgg cccaatcgga cgtggtcgtc
     7861 tttgcagtcc cccttcataa gaccgtggag atcattcgag acctggtgcc ctacacccgg
     7921 tccgatcagc tcctcatgga cgtgagcagc ctcaagacgg cgcccatccg ggagatgctc
     7981 gcctcctccg catccgtggt ggggctccat cccatgttcg ggggccgcat ctcctccttc
     8041 gaaggacaga ccctggtggc ctgtcccgtc cggatctccc cccggaactg ggccgaagtg
     8101 cgcgggatgt tcacccgggc gggaattcgc gtcaaggaaa gcgatccgga agagcatgac
     8161 cggatgatga gcatcatcca ggtgctcttt cacatgacca ccatgctcaa gggcagagtg
     8221 ctccgggaac tgggggtgga catcgaggac accctcgagt acacgagccc cagctatcgc
     8281 ctggaaatca accttctggg gcgaatgttt gcccaaaacg ggagcctgta ttcggccatc
     8341 gcccagatga acccctatac ccgcgacatc ctggctcacc tgcaccaggg cttggacgcc
     8401 tacgaacgct ggtacgggga
//
LOCUS       DAOOVC010000163         8392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5194_length_8392_cov_8.390428, whole genome shotgun sequence.
ACCESSION   DAOOVC010000163 DAOOVC010000000
VERSION     DAOOVC010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5194_length_8392_cov_8.390428"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1090..2799
                     /locus_tag="PLM79_15495"
     CDS             1090..2799
                     /locus_tag="PLM79_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOV87764.1"
                     /translation="MRLTPLIRNKARELGHDPVKIHHFVRNRIEFSPTYGSLQDAETT
                     LLNGRGNAADISTLLIALLRASGISCRYVEGSIEVSAESFRNWTGGLESTEGALDLAA
                     SGGIPVTGLASGGRIGRVRMEHLWVEAWIDYAPSRGSEHREGDTWIPLDAAYKEMNLT
                     SAADLTRDLPPVLEALLDGWVAGPGDSGAAALSYLTLAGSGIEELAPGISGGYEPVLS
                     AVGSFLFAAAAVHTALIDLAVSRAAGSPDCSCLSEPRPPECDSPREFPVPRDDPLEGP
                     DPCLPDTDQDRRFPAGSEADLSHKEYNLRVTDLSVKVPGGSLEITRRYEADRWVFDHN
                     RFRVVPKCGDLPGCTTVHRVRLNGAEYEPVGGDERLFFRETSQITRDDQDRYTWRDKE
                     GISTRPGRPPGFRGWKTETEGRSFGSSTRGGPGALGGAGPCGSKGRVRLPRGASALGS
                     RRFGKCYELFLRLPGKTRYDHGSFGQPAHRGIRSLGRSRLGDGRTGGGVFFPVLLRRG
                     NQGILLPDHRLLGAGQGGLVRSGRGHEKGGPRGKDRKADPQGGERLPGRRRAGPHHPK
                     GAR"
     gene            2822..4915
                     /locus_tag="PLM79_15500"
     CDS             2822..4915
                     /locus_tag="PLM79_15500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87765.1"
                     /translation="MHPDGREVSYAYEPVFNRRTEKTDERGILTRHSYDASGNLTETV
                     EAANTDSPRTTRYGYDSDANLLFREETGDEKTAPALTRYAYDSWGNRVQEIDPEGGTV
                     AYTHDVGGNVLTRTDALGNRHNFGYDLSGRLIEASDPLGNRKTFSYVAAGNLAKRIDP
                     GGSEWTLSHDERGRPVEIGDPLGASTRYSYNPDGKRTEFQNARGNIFRYVYDPRGRLS
                     RTVDGNGNEVLFHYDGSSGCGSCSFGADRPSRIVFPTFAREFEYDPRGRMTRERKVLS
                     PDTIYEAVYAYDRAGNLVSATDREGKTTRYSYDVLGRPAEVLDAAGGIARYGFDARDN
                     LLLFTDPAGKTTQFSYDRNNRPGKEVRPMGRETLYSWDPAGRLVRKVDPRGQKTEYGY
                     DDGGRLVEIRSLETPGPPSPHKTVSFSYSPTGNLLGYEDGLTSGRFVYDAAYRKIGET
                     LRYGSLELSASISRYPGGLKKSYTGPDGICYEYTYDGNNRVRSVEIPGEGTLLFESYL
                     WLRPTRVRLPGGVLREYSYDPLLRLASTAVKTPSGQIVLGSRHEYDREDHLVLRETED
                     GLETYGYDDLYRLVWADHPVLGRREFGYDAAGNRTSDTRVPGPWSYNDNHELVSYGNV
                     RQEYDAAGNLVRETRELPSGGSRVRGLGLRRRKPPPAGGKRGRGGPFLLPVRSFRPKA
                     REGDLGGPRLLFLRR"
     gene            5022..5348
                     /locus_tag="PLM79_15505"
     CDS             5022..5348
                     /locus_tag="PLM79_15505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOV87766.1"
                     /translation="MHHFYHNDHLGTPMKLTAPEGALSWSARYAPFGLAFPDPASPVP
                     NPLRLPGQYFDPETGLHYNLHRYYDPNTGRNLTRDPIGLEGGMNPYGYAENVPVNLVD
                     PWGLWA"
     gene            complement(5670..5921)
                     /locus_tag="PLM79_15510"
     CDS             complement(5670..5921)
                     /locus_tag="PLM79_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87767.1"
                     /translation="MEITVYNPPKGRLETIAVELTKDNTTWFESYRNSSDIAGITDFE
                     GGLLITEFDYTHPVWIYDTSRADIGYSQKKARQLLRMHV"
     gene            complement(5938..6654)
                     /gene="istB"
                     /locus_tag="PLM79_15515"
     CDS             complement(5938..6654)
                     /gene="istB"
                     /locus_tag="PLM79_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOV87768.1"
                     /translation="MRLPNMARNLEGLLRQAKESGIGYDDFLLDLTGAELQSRAESRL
                     NRRIKESRFPLLKPLETFDLAAVPDLDIRLFRELAGCDYIRELRNVIFLGRSGTGKTH
                     MAAALGIEACKNNFRTRFVTCYGLVNELVEAREERTLQRLIQKVVRCDLLVLDELGYI
                     PFSKEGSALLFHVLAERNEKGSVIITTNLGFAHWTQVFGDATMTAALLDRLTHKAHII
                     NCSWESYRLKQSLSRKSKGA"
     gene            complement(6696..7808)
                     /locus_tag="PLM79_15520"
     CDS             complement(6696..7808)
                     /locus_tag="PLM79_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87769.1"
                     /translation="MDWGTAVAILSGEEVRLKFFCMRSKYSEKHFVRLYPCERQQAFF
                     DAHARAFCFFGGVFPVLIYDNLTSEVRKGLRGLDRVEQQQFCKLKAYYSFEARFCNPD
                     SGHEKGGVEGLVGFARRNYLVPVPEAANLDELNEKILDRCVAYGSRKTAGRDRTVNAL
                     CEEEKSHLVALPEEVFSNVRTGDGRADKYATVIVDKNRYSVPSRCAGFRVKVPLYVDR
                     VEIFSGAKKVATHGRFYGNNKWCLNPDHYLDLIQQRPMAFKSTRPIRQWQESWPESLH
                     VLLQRFCSAQGETKGIKDFVTVLMFYRDHESAEVEAAVKLAAESHISTSEGVRHILTS
                     SANPASAIAPLSATSLPWSTPAPPDVAVYGQLGGVQ"
     gene            complement(7809..8198)
                     /locus_tag="PLM79_15525"
     CDS             complement(7809..8198)
                     /locus_tag="PLM79_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87770.1"
                     /translation="MDQYELVRTGYRVYGKTISELARLTGHSRNTIKKAIRGEPWGYK
                     ERAHQPFPILGDYADMIDQWIESDKDKPGKQRHTARRIYHRLVEEHGFEGSESNVRRY
                     VRFAKMRLGIAGSGAFIPCDPEAGNEA"
BASE COUNT         1634 a         2554 c         2577 g         1627 t
ORIGIN      
        1 atttgctctt gacttggggc aagagttttg tctaggatcg ttttcatgtc gattgggcat
       61 tccccgggag cacccgttcc cgccggggga atccgccggg agtcttcaaa cgatccaacc
      121 attttcctta cacgacccgg aagaaaacgc cgcgctcttt gccgtgggag aggccggacc
      181 ttttttgtga ggccgggtcg ccgcaaagca tcataccgga agggcctcgt cgtggaaaca
      241 cccgcaagag gggggggact gtcggagctc cgggaaaggt ccccgggcca agtgtttttg
      301 agatcaggcg aagcgtttcg aagggagggg aggcagatgg gttcggggct ttccgggttt
      361 gggggattcg gcgggccaag gggccgtttt ggggaaagaa gagggttcgg gcgggccgaa
      421 tgaagcgccc ggggaccttt cggggcgggc tttgcccggc agttgcggcg gcccttttct
      481 tggcgggagg ttccctgctt tttcccgcag cggcgcgggc ggcggatgtc ggggggcttt
      541 cggtcaggaa aggcttcaag ccgaacccgc cgagtggctg aaggacctgt cccgggtcct
      601 ttcggatccc gcccgaagcc gtgatgagaa gaaggaaaga gtgaaagagg ccgggggccg
      661 ggtcttttcc ctggagcggg agatgtcccg gagcctgtct cggctggaaa agagtcttca
      721 ggcctcgggc tatccctcgg aggccttggc ccggcaccgg gagttcggga aaaggtgccg
      781 aaaaaagctc cgaaagctgg ccggggagct ggaaaaagcc gcctcggcaa gctccctttc
      841 ggctgcaaga caaagcctgg ataaggccct ttgggcctgg gaaccgaggc ggcggcggat
      901 cgaggccgaa ggaccccggc cgggggacct ttcccaccgg gcgggggagc cccgaagggt
      961 tcttccccgg gaaagccccg gggagtttct cccaaagagg gcctctccgg gggcaaaatc
     1021 tcccggggcg gcaaagaccc tcgagtacgg gggccccccg ggcccgagga cctttgggaa
     1081 acccccgagg tgcgcctcac tccccttatt cgaaacaagg cccgcgagct cggacacgat
     1141 cccgtaaaga tccatcattt cgttcgaaac cgcatcgagt tttcccccac ctacgggtcc
     1201 ctgcaggacg cggaaaccac cctgctgaac ggccggggaa acgccgcgga catctcgact
     1261 cttctcatcg cccttcttcg ggcctcgggg atttcctgcc gctacgtgga aggaagcatc
     1321 gaggtttccg cggaatcctt ccggaattgg acggggggcc tcgagtcgac ggaaggcgcc
     1381 ctggaccttg ccgcctcggg gggaattccc gtaacgggcc tggcctccgg gggccggatc
     1441 ggccgggtcc gcatggaaca cctgtgggtg gaggcctgga tcgattatgc tccctcccgg
     1501 ggctccgaac accgggaagg agacacctgg attcccctgg acgcggccta caaggaaatg
     1561 aatctcacct cggccgccga tctcacccgg gaccttcccc cggtcctcga ggctttgctc
     1621 gacgggtggg tggccgggcc gggagattcg ggggcggcgg ccttgagtta ccttaccctt
     1681 gcggggtccg ggatcgagga gctcgccccg ggaatttccg gaggatacga gcccgtcctt
     1741 tccgccgtgg ggtcctttct ctttgcggcg gcggccgtcc atacggccct catcgatctt
     1801 gccgtgtccc gggccgcggg gtcccccgac tgttcgtgtc tctcggagcc ccggcccccc
     1861 gagtgcgact ccccccggga atttcccgtt ccccgggacg atcccctgga aggacccgac
     1921 ccgtgccttc cggacacgga ccaagaccgg cgctttccgg cgggctccga ggcggacctt
     1981 tcccacaagg aatacaacct ccgggtgacg gacctttcgg tcaaggtccc gggagggtcc
     2041 ctcgagatca cccgccgcta cgaggccgac cggtgggtct tcgatcacaa tcggtttcgg
     2101 gtggtcccca agtgcgggga ccttcccggc tgcacaaccg ttcatcgagt ccgtctcaac
     2161 ggagcggagt acgaaccggt gggcggagac gaacgtcttt tcttccggga gacctcccaa
     2221 atcacccggg acgatcagga ccgctacacg tggcgggaca aggaaggtat ctctactcga
     2281 ccgggtcgtc ccccagggtt tcgggggtgg aagaccgaaa cggaaggcag gtcttttggt
     2341 tcgagtacga ggggggggcc gggggccctc ggcggtgcgg gaccttgcgg gtcgaagggt
     2401 cgagtacggc tgcctcgagg ggcttctgcg ctcggttcga gacgtttcgg gaaatgttac
     2461 gagctattcc tacgactccc agggaagact cgttacgatc acggatcctt tgggcaaccg
     2521 gcgcaccgtg ggatacggtc cctggggaga agtcgcctcg gtgacggacg cacggggggc
     2581 ggggtatttt ttccggttct ccttcgacga ggaaaccaag gaatcctatt gccggatcac
     2641 cggctcctcg gggctggtca aggaggtctg gttcgatcgg gtcggggaca cgaaaagggt
     2701 ggccctcggg ggaaagaccg taaagcggat cctcaaggag gagaacgcct accgggtcgt
     2761 cgacgagcag ggccgcatca cccgaaagga gctcgatgag cgaaaaaacg tggttcgggt
     2821 ggtgcatccc gacggccgcg aggtttccta cgcctacgag cccgtcttca accgcagaac
     2881 ggaaaagacc gacgagcggg gaatcctcac ccgccattcc tacgacgcct cgggaaacct
     2941 tacggaaacc gtcgaggccg ccaacaccga ttccccccgg acgacccgct acggttacga
     3001 ttccgacgca aatctcctgt ttcgggaaga gacgggagac gaaaaaacgg ccccggccct
     3061 cactcgatac gcgtacgatt cctggggaaa ccgggtccaa gaaatcgatc ccgaaggagg
     3121 gaccgtcgcc tacacccacg atgttggggg aaacgtgctc acccgcacgg acgccctggg
     3181 aaaccggcac aatttcggct acgatctttc ggggcggctg atcgaagcct cggatcccct
     3241 gggaaacagg aagacctttt cctacgttgc ggcgggaaac ctcgcaaaaa ggatcgaccc
     3301 cggggggagc gagtggactc tttcccatga cgaacgagga agaccggtgg agatcgggga
     3361 ccctttggga gcgagtaccc ggtattccta caacccggac ggaaagagga cggagtttca
     3421 aaacgcccgg ggaaacatct tccgctacgt ctacgatccc cggggacggc tttctcggac
     3481 cgtggacgga aacggaaacg aggtcctttt ccactacgac gggtcctcgg gctgcgggag
     3541 ctgttccttc ggggcggatc ggccttcccg gatcgttttt cccaccttcg cccgggagtt
     3601 cgaatacgac cctcggggca ggatgacccg ggagaggaag gtcctttccc cggacacaat
     3661 ttatgaggcc gtctatgcct acgacagggc cggaaacttg gtttcggcaa cggaccggga
     3721 gggcaaaacc acccggtatt cctacgacgt cctgggacgc ccggccgaag ttttggatgc
     3781 cgccggggga atcgcccggt acggcttcga tgcaagggac aatctcctcc tgtttacgga
     3841 cccggcggga aagaccacgc agttttccta cgaccggaac aaccggccgg gaaaggaggt
     3901 gcggcccatg gggcgggaaa ccctgtattc gtgggacccg gcgggtcgcc tggtccgaaa
     3961 ggtcgatccc cgggggcaaa aaaccgaata cggctacgac gacgggggaa gactcgtcga
     4021 gattcgatcc ctcgagactc cgggtccccc ttctccccac aagaccgtct ccttttccta
     4081 cagcccgacg ggaaaccttt tgggctacga ggacggcctc acctcggggc ggttcgtcta
     4141 cgatgcggct taccgcaaaa tcggggaaac cctccggtac gggtccctcg agctctccgc
     4201 aagcatctcc cggtatcccg gcgggctcaa gaagagctac acgggacccg acggaatctg
     4261 ctacgaatac acctacgacg gaaacaaccg ggttcggtcc gtggaaatcc ccggggaggg
     4321 gacccttctt tttgaatcct atctttggct tcggccgacc cgggtgcgcc ttcccggagg
     4381 agtcctccgg gaatactcct acgatcccct cctgcgattg gcctcgacgg cggtaaagac
     4441 cccttcgggc caaatcgtcc tggggtcccg ccacgagtac gaccgggagg accacctggt
     4501 cttgcgggaa accgaagacg ggctcgaaac ctacggctac gacgatttgt accggctggt
     4561 ttgggcggac catccggtcc tgggaagacg ggagttcggc tacgacgccg cgggaaaccg
     4621 aacctccgac acccgggtcc ccggcccctg gtcctacaac gacaaccacg aactggtctc
     4681 ctacggaaac gtgcggcagg aatacgacgc ggcaggaaac ctcgtgcggg agacccggga
     4741 attgccctcg ggaggctccc gggtgcgggg tcttggtcta cgacggcgaa aaccgcctcc
     4801 tgcgggtgga aaacgaggcc ggggaggtcc tttcctccta ccggtacgat cctttcggcc
     4861 gaaggctcgg gaaggagatc tcgggggtcc ccgccttctt tttttacgcc gatgaaggcc
     4921 tcgtgggaga atacggcccg tcgggaaaag aactccgaac ctaggggtat ctgcccgggc
     4981 cccttttcac caccggcccg gtctttttga aacagggggg gatgcatcat ttttaccaca
     5041 acgaccatct gggcaccccg atgaagctca ccgcccccga gggcgccctg agctggtccg
     5101 cccgttacgc tcccttcggc ctcgccttcc ccgaccccgc ctccccggtc ccaaaccccc
     5161 tccgccttcc cggacaatac ttcgaccccg aaacgggact ccactacaac ctccaccgct
     5221 actacgaccc gaacacaggc agaaacctga cccgggaccc catcggactc gaagggggca
     5281 tgaatccgta tgggtatgct gagaatgttc cggtgaacct tgtcgatccg tgggggttgt
     5341 gggcatagct gtggactctg gtggtgctta cgcgactggc tggggtggtc aggtcaatcc
     5401 aattgctgcc attcttcaag gtggttctgc tggtaccgga ttttatttgg gaacgaaggg
     5461 agtaccaggt tacgcgggtt tcggaatgac aatcgaaatt tgaccatctg cgataaccga
     5521 aaattgacca cccttgagca gagagaatcg tcatgggaaa tgggggggtg atcatcctcg
     5581 tgccaccatg ggaccggcag gtgaaacgga gggagcccgg ggggcgaccg gagtttcacc
     5641 tgccggcggg tccaatcgcg gtgccggatt tacacgtgca tccgcagcaa ttgccttgct
     5701 tttttctggc tgtagccgat gtcagccctc gatgtatcgt agatccagac gggatgggtg
     5761 taatcaaatt cagtaatcag caggccgcct tcaaaatccg ttattccggc aatatcactg
     5821 gaattccggt aactttcgaa ccaggttgtg ttgtccttgg ttaattcgac tgcgatcgtc
     5881 tcgagtcgcc cttttggggg gttgtaaacg gtgatctcca taggtcgtgg ctcctcgcta
     5941 tgcacccttt gatttccgac tgaggctttg tttgagccgg tagctctccc agctgcaatt
     6001 gatgatgtga gccttatggg tcaaccggtc caggagtgcg gcggtcatgg tcgcatcgcc
     6061 gaaaacctgg gtccagtggg cgaacccgag attggtcgtg atgatcaccg atcccttctc
     6121 gttccgttcg gccaggacat gaaacagcag tgcggagcct tccttggaaa acgggatgta
     6181 gccaagctcg tccaggacca gcaaatcgca ccgaacgacc ttttggatga ggcgctgcag
     6241 ggttctttct tctcttgcct cgaccagctc attgaccagg ccgtagcagg tgacgaaacg
     6301 ggtccggaaa ttgttcttgc aggcctcgat ccccagagcg gcggccatgt gtgttttgcc
     6361 ggtgccgctt cgcccgagga agatgacatt tctgagctcc cggatgtagt cgcacccggc
     6421 aagctcccga aacaggcgga tatcgagatc cgggacagcc gccaggtcaa aggtttcgag
     6481 gggcttcaaa agcggaaacc tcgattcctt gatgcgacga ttcagcctgc tttccgccct
     6541 ggactgcagc tccgctccgg tcagatccag gagaaagtca tcgtagccga ttccgctctc
     6601 tttggcctgt cgtaggagcc cttccaggtt ccgagccata ttgggaaggc gcagcgtttt
     6661 gagattcgct cacagctcga tcagggtgcc gactgtcatt gcacacctcc caactgtccg
     6721 tagacggcca catcgggagg agccggagtc gaccagggca gtgaggtcgc gctcagggga
     6781 gcgatggctg aagcgggatt ggcggagctg gtcaaaatgt ggcgcactcc ctcgctggtg
     6841 ctgatgtggc tctccgcggc gagcttgacg gcggcttcaa cctcggcgga ttcatgatcc
     6901 cggtagaaca tgagaaccgt cacgaagtcc ttgatcccct tggtttcacc ctgggcgctg
     6961 caaaaacgct gcagaagaac gtgcagggat tccggccagc tctcctgcca ctgtcgaatg
     7021 gggcgggtgc tcttgaaagc catgggccgc tgctgaatca ggtccagata gtggtccgga
     7081 ttcaagcacc atttgttgtt gccgtaaaac ctcccgtgcg tcgccacctt cttggcgccg
     7141 ctgaaaatct cgacccggtc cacataaagc ggcactttta ccctgaaccc cgcgcaacgg
     7201 ctcggaaccg aatagcggtt cttgtccacg atgaccgtgg cgtacttgtc ggcccggccg
     7261 tcgccggtcc ggacgttgct gaagacttcc tcgggtaaag cgacaagatg actcttctcc
     7321 tcctcgcaca acgcgtttac cgtacggtct cgaccggccg tcttgcggct tccgtaagca
     7381 acgcagcggt cgagaatctt ctcgttgagc tcatcgaggt ttgcggcctc cggaaccgga
     7441 accaggtagt tgcgccgtgc aaaccccacc aacccttcga ccccgccttt ctcgtgaccg
     7501 ctgtcggggt tgcaaaaacg ggcttcaaaa ctgtagtagg ccttgagctt gcagaactgc
     7561 tgctgttcca cgcgatccag gcctcgcaac cccttccgga cttcgctggt cagattgtca
     7621 tagatcagaa cggggaaaac cccgccaaag aagcaaaagg cccgtgcgtg ggcatcgaaa
     7681 aacgcctgct gacgctcgca ggggtaaagc cggacgaagt gcttttcgga atacttgctc
     7741 cgcatgcaga aaaacttcag ccggacttct tctccagaga ggatcgccac ggccgtcccc
     7801 cagtccacct aggcctcatt cccagcctcc gggtcgcagg gaatgaaggc accggatccg
     7861 gcaatcccca gcctcatctt cgcgaatctc acatatcggc ggacattcga ttcactcccc
     7921 tcaaagccat gctcttcaac gagtcgatgg tagatccgcc gtgccgtgtg gcgctgcttt
     7981 cctggcttgt ccttatcgct ctcaatccac tgatcgatca tgtccgcata atcgcccaga
     8041 atcgggaacg gctgatgagc ccgctcctta tagccccatg gctcgcctcg tattgccttc
     8101 ttgatcgtgt tacgcgaatg ccccgtcagc ctcgccagct cactgatcgt ctttccgtag
     8161 acccgatacc ctgtccttac cagttcatac tgatccatct tcagcactcc tttccttcca
     8221 atgtggttgc tcgaaaaaaa acacgagcca ctctacatgg agaggtgccg ctcaaggtgg
     8281 tcaattttcg gtgatcgtta cccccctttt ctggtcaatt ttggattatc aaaaccagca
     8341 ggatcccgaa aaacgccacc ccacggcaga ggatggagcg aaagctcacg ac
//
LOCUS       DAOOVC010000164         8081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5393_length_8081_cov_5.655495, whole genome shotgun sequence.
ACCESSION   DAOOVC010000164 DAOOVC010000000
VERSION     DAOOVC010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_8081_cov_5.655495"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /gene="pyk"
                     /locus_tag="PLM79_15530"
     CDS             complement(<1..1079)
                     /gene="pyk"
                     /locus_tag="PLM79_15530"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOV87771.1"
                     /translation="MTLPLNKTKIVCTIGPASQSRAVMEELLLAGMNVARLNFSHGDF
                     SGHKNVIEDLRAASEATGRRLAIMADLPGPKMRIGHFDSEPVFLKPGDPFILTTEDIV
                     GDERRVSMSFAPLPRVVKPGDTLYLNDGLIQLEVRTADGTDVHCRVVVGGELRSRKGL
                     NLPGIDLGISAFTEHDHNCLKFAMENGVDAVSQSFVESAADIRAVRNAAAALGHHPFI
                     IAKIERSTALHYLDEIITEADAVMIARGDLGVELPIEQIPVVQKQIMRKANLWAKPVI
                     TATQMLESMTTNRRPTRAEATDVANAILDGTDAVMLSGESAMGRYPVDSAAMLAKIAA
                     AIEPYRTGIRAGELFRGIDLKGKLPP"
     gene            complement(1272..2489)
                     /locus_tag="PLM79_15535"
     CDS             complement(1272..2489)
                     /locus_tag="PLM79_15535"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOV87772.1"
                     /translation="MRVVVVNSGSSSIKYEVFALQDCSLVVKGLLERIGTREATFRRR
                     WLSDSGAWEELVETRPIADHHEGFEFLLEVAVRYPTTRVAPKAFFGFGHRVVHGGERF
                     RAPVIVDDEVVRQIKDLIPLAPLHNPSNVLAIEAVRTLRPDLPNVAVFDTAFHQSMPP
                     RAFHYALPNELYGMHHVRRYGFHGTSHHYVAKEASRYLGIPEEHLNLVTLHLGNGASA
                     TAVEGGKSVDTSMGLTPLEGLIMGTRCGDLDPAIHFYLSRKIGRPFEEIESVLNKESG
                     LKGICGLNDMREIQRRAEEGDDRAQLAIEMFCYRIKKYIGAYCAVLGRVDAVVFTGGI
                     GENSARIRKESCEGLESLGICLDDRKNAESFRGIAEIQAGESRVKVLVIPTDEELEIA
                     RQTIETIRRARGA"
     gene            complement(2486..4588)
                     /gene="pta"
                     /locus_tag="PLM79_15540"
     CDS             complement(2486..4588)
                     /gene="pta"
                     /locus_tag="PLM79_15540"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOV87773.1"
                     /translation="MSRNLFITGMGPGSGKSAVVLGMMEMLARHGRKLGFFRPVVEGG
                     ANPDPLSALVKSRYPVVSTGEHMFGVDYRTARDLIAEESLEELYSRILEKYAALAGEC
                     DFVLCVGTDYRTVHTTLEFEFNVEVARNLGAPLVPVVVGQGRTVRQILDSVWVLVDSL
                     EANNCDILSVFVNRVSPGDVTDVVNGFREDAPAAGFPLFVLPAHPLLDKPTVAEIRKA
                     LGAEMLHGDPARLDREVAHYKVAAMELPNFLDRLEEGSLVITPGDRSDILIGALAADA
                     ATSYPGIAGVVLTGGFGPAPQVLRLFKGLKRSPVPILDVKLDTFSTASRVSSIEASLT
                     PENSRKIAGALGLFESNVDPALLDHRIDVMRSSRITPLMFQHDLLSRARSRRRHIVLP
                     EGVEERVLRAAEILRLRDVADLTLLGNAVEVRDKITALGLSLGEVEIIDPAASELREE
                     FGRTYYELRKHKGIPLQVALDAMNDVSCFGTMMVYRGIADGMVSGATHTTQHTIRPAL
                     EFIRTRPGCSIVSSVFFMCLPDRVLLYGDCAINPDPDAEQLAHIAVSSAMTAESFGIE
                     PRVAMLSYSTGESGKGTNVDKIKEAARVARQLRPDIKLEGPIQYDAAIDPEVAKIKLP
                     GSEVAGRATVFIFPDLNAGNNAYKAVQRAANAVAIGPVLQGLNKPVNDLSRGCTVADI
                     VNTVAITAIQAQLEGDGS"
     gene            complement(4663..6717)
                     /locus_tag="PLM79_15545"
     CDS             complement(4663..6717)
                     /locus_tag="PLM79_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase"
                     /protein_id="HOV87774.1"
                     /translation="MTGPVSSADEWERTEGAPWPPGASWVEAEKAYNFALFSRHARSV
                     TLLFYTERDPANPVHSRVLDPVLNRTGLVWHCRVPEEELCGAALYAYRVDGPYDPSRG
                     HRFDPQKVLLDPFASSVYFPPGYSRSAAMEPGPTDGKAPLGRLSRGQKPFDWGTAPPP
                     RHTHDLIVYELHVRGFTARANSGVSRDKRGTFAGLTEKIPYLKDLGITAVELLPIHQF
                     DPQEGNYWGYMTLNFFAPHQGYASGDPDGEFRSMVKAFHEAGIEVWLDVVYNHTSEGD
                     ETGPTYSYRGIDNRSYYLTRRDSGEYFNDTGCGNTLNCAHPVVRALVLCSLQHWAIDM
                     RVDGFRFDLASIFTRTLDGSVNTVDPPLIAEISLFGYFREVRLVAEAWDINSYLLGRS
                     FPGLMWRQWNGKFRDDVRAFVKGDPGMVGALMQRLYGSDDLFPEGPVEVYRPYQSVNF
                     ITAHDGFCLYDLTAYNEKHNEANGHDNTDGTNNNLSWNCGWEGDSGVPEEVMTLRRRQ
                     VKNFFCLLMLANGTPMFCAGDEFMNTQKGNNNPYNQDNEITWLDWDLLEKNRDLFRFF
                     RLMIAFRKARPSIGRGRYWRGDVRWYGPTGGVDLSRDSRTLAYCLGGQSLKDKDLYVM
                     INAYWEDLEFTVQEGRADEWLRIVDTGLPSPEDIAEPGKGQLVPSLEYRVRARSIVIL
                     ER"
     gene            complement(6906..7943)
                     /gene="fbaA"
                     /locus_tag="PLM79_15550"
     CDS             complement(6906..7943)
                     /gene="fbaA"
                     /locus_tag="PLM79_15550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOV87775.1"
                     /translation="MPVADYKTYCKMLDRAKENRFAYPAINVSSLTTANAVLKGLAES
                     RSDGILQVSTGGGAFASGSSVKDMALGAISIAEHVHRVADRYNIFVALHTDHCQPQVL
                     DKFVLPLVEETERRRKAGLPNLFNSHMFDGSALPLKENLDIAVPLLERCRRNELILEV
                     EAGVVGGEEDGIKAEGAPAEKLYTTPEDMVEVARRLGSVPGARFLLAATFGNVHGVYK
                     PGHVKLKPSILKDGQDAVENVYGKDARFYLVFHGGSGSLPEEIHEAIDYGVVKMNIDT
                     DTQYAFTRAVADHMFRNYDGVLKVDGEVGNKKTYDPRSYLGSAEAAMAERVKAAVVAL
                     RGVGTTLCTAK"
BASE COUNT         1436 a         2477 c         2572 g         1596 t
ORIGIN      
        1 gggggtaact tgcccttgag gtcgatgcct cggaacagtt cgccggcccg gatcccggtc
       61 ctgtagggtt cgatggcggc ggcgatcttg gcgagcatgg cggcggaatc cacgggatac
      121 cggcccatgg cggattcccc cgagagcatg acggcatcgg tgccgtcgag aatggcgttg
      181 gccacgtccg tggcctctgc acgggtgggc cgccggttgg tggtcatgga ttccagcatc
      241 tgggttgccg tgatgacggg cttggcccat aggtttgctt tgcgcatgat ctgtttctgc
      301 accacgggga tctgttcgat gggcagctcg acgcccaggt ccccccgggc gatcatgacg
      361 gcgtccgcct cggtgatgat ctcgtccagg taatggagag ccgtcgatcg ttcgatcttg
      421 gcgatgatga aggggtggtg acccagggcc gccgcggcgt tgcgcaccgc ccggatgtcc
      481 gcggcgctct cgacgaagga ttggctcacc gcgtccacgc cgttttccat agcaaacttc
      541 aaacagttgt ggtcatgttc cgtgaatgcg ctgatgccca ggtcgatgcc cgggagattg
      601 agccccttgc gcgagcgcag ttccccgccc acaaccaccc ggcagtggac gtcggtgccg
      661 tccgccgtgc gcacctcgag ctggatcaag ccgtcgttca gatacagggt gtctcccggc
      721 ttcaccaccc ggggaagggg ggcgaaagac atggagaccc ggcgctcatc gcccacgatg
      781 tcttcggtgg tgaggataaa cgggtctcca ggtttcagga agacgggttc ggagtcgaag
      841 tgcccgatcc gcatcttggg gccgggcagg tcggccatga tggcaagacg gcgcccggta
      901 gcctcggatg ccgcccggag gtcctcgatc acgttcttgt ggccggagaa atccccgtgc
      961 gaaaaattga gccgagccac gttcatcccg gcaaggagca attcctccat gaccgcacgg
     1021 gactgggagg caggtcctat ggtgcacacg atcttggtct tgttcaaagg cagggtcatc
     1081 gacgtcctcc acggataggg tgccgcatcg gcacgagatg tctcacggca agtacggagg
     1141 cggggttccc ggcggcctcc gcgattcgtt tcccgtcacc gggacagccg gcccggatcg
     1201 tccccgcccc gccgtctttc agcgggtcgg cgacgtgaaa ccctcgtgtg tcgatcatgg
     1261 cacggattcc tttaggcccc ccgggcacgg cggatggttt caatggtctg gcgcgcgatc
     1321 tccagttctt cgtccgtcgg gatcaccagg accttgaccc ggctctcccc ggcctggatc
     1381 tccgcaattc cccggaacga ttcggcgttt tttcgatcat ccaggcagat gcccagggac
     1441 tcgaggccct cgcaggactc ttttcgaatg cgggcggaat tctccccgat cccccccgta
     1501 aagaccacgg cgtccacccg gcccaggacc gcgcaataag cgccgatgta cttcttgatg
     1561 cggtagcaga acatctcgat ggccagttgc gcccggtcgt ccccttcctc ggcgcggcgt
     1621 tggatttccc gcatgtcgtt caggccgcag atgcctttga ggccgctttc cttgttcaaa
     1681 acggactcga tctcctcgaa cggccggcca atttttctcg atagatagaa gtgaatggcg
     1741 ggatccaggt ccccgcagcg ggtgcccatg atcaggccct cgaggggcgt gagtcccatg
     1801 gaggtatcca cgctttttcc gccctccacg gcggtggcgc tggccccgtt gcccaggtgg
     1861 agggtgacca ggttcaggtg ttcttccggg attcccaggt agcgggaggc ctcctttgcc
     1921 acatagtgat gggaggtccc gtggaaaccg tagcggcgta catggtgcat cccgtaaagt
     1981 tcgttgggca acgcatagtg aaacgccctg ggcggcatgc tctggtggaa ggccgtatcg
     2041 aagaccgcca cgttgggaag atcggggcgc agagtgcgga ccgcttcgat ggccagcaca
     2101 ttggacgggt tgtggagggg agccaggggg atcaggtcct tgatttggcg caccacctca
     2161 tcatccacga tcaccggtgc ccggaacctc tctcccccgt ggaccacccg gtggccgaag
     2221 ccgaaaaacg ctttcggggc cacccgggtg gtggggtagc gcacggcgac ctcgagaagg
     2281 aactcgaacc cttcgtggtg gtcggcgatg ggccgtgttt cgacaagttc ctcccatgcg
     2341 ccggaatcgg aaagccagcg ccgccgaaag gtggcttccc gggtgccgat ccgctcgagc
     2401 aaccctttca ccaccaggct gcaatcctgc agggcaaaga cttcgtactt gatggaagag
     2461 ctgccggaat tgacaacgac caccctcatg agccgtctcc ttccagttgt gcctggatgg
     2521 cggtgatggc aacggtattg acgatgtccg ccaccgtgca tccgcgactc agatcgttga
     2581 ccggtttgtt gaggccctgg aggacgggtc cgatggcgac ggcgtttgcc gcccgctgga
     2641 cggctttata agcattgttc ccggcgttga ggtcgggaaa gatgaaaaca gtggcacggc
     2701 cggccacttc actgccggga agcttgatct tggcaacctc cggatcgatg gcggcatcgt
     2761 actgaatggg tccttccaat ttgatgtcgg ggcgaagttg ccgggcgact cgggcggctt
     2821 cctttatctt gtccacattg gttcccttgc cggattcccc cgtggagtag gaaagcatgg
     2881 ccacccgggg ttcgattccg aagctctcgg cggtcatggc ggaactgacg gcaatgtggg
     2941 caagctgttc ggcatcggga tccggattga tggcgcagtc gccgtagagc agaacgcggt
     3001 cgggaaggca catgaaaaag acactcgata cgatggaaca gccgggccgg gtccggatga
     3061 actcgagggc cgggcgaatg gtgtgctggg tcgtgtgggt cgctccggag accatgccgt
     3121 cggcaatccc gcggtacacc atcatggtgc cgaagcagct cacgtcgttc atggcgtcca
     3181 gggccacctg gagcggaatt cccttgtgtt ttcgaagctc gtagtaggtc ctcccgaatt
     3241 cctctcgaag ctccgaggcg gcgggatcga tgatttccac ctcgcccagg gacaacccca
     3301 gggccgtgat cttgtcccgg acctcgacgg cgtttcccag cagggtgagg tccgccacgt
     3361 cccgcaggcg caggatttcc gccgcccgga ggacccgctc ctcgacgcct tcgggaagaa
     3421 caatgtgcct gcgccgggac cgtgcccggc tcagcaggtc gtgctggaac atgaggggcg
     3481 tgatgcggga ggacctcatg acgtcgatgc gatggtccag gagggcaggg tccacgttgg
     3541 attcgaacag tcccagggct ccggcgatct ttctggaatt ctccggggtg aggctcgcct
     3601 cgatggagct cacccgggat gccgtcgaaa aggtgtcgag cttcacgtcc aggatgggga
     3661 ccggcgagcg tttcaggcct ttgaagagac gcagcacctg gggggcaggc ccaaacccgc
     3721 cggtaaggac cactccggca atccccgggt aagaggttgc cgcgtcggcg gctagtgccc
     3781 cgatgagaat gtccgagcgg tccccggggg tgatgaccag gctgccttct tccaggcggt
     3841 ccaggaagtt gggcagttcc atggccgcca ccttgtaatg agccacctcc cggtccagcc
     3901 tcgcggggtc tccatggagc atttcagccc cgagggcctt ccggatttcc gccaccgtgg
     3961 gcttgtccag gagaggatgg gcgggtagga cgaagagggg aaacccggcg gccggtgcgt
     4021 cctcccgaaa cccgttcacc acgtcggtca cgtccccggg ggaaacccgg ttgacgaaca
     4081 ccgagaggat atcgcagttg ttcgcctcga gggaatccac cagaacccag acgctgtcga
     4141 gaatctggcg gaccgttcgc ccctgtccca cgaccacggg caccaagggt gcgcccaggt
     4201 tgcgggccac ctcgacgttg aactcgaatt cgagggtcgt gtgcacggtt cgataatcgg
     4261 tacccacgca caggacaaag tcgcattctc cggccagggc cgcgtatttc tcgagaatcc
     4321 ggctgtatag ctcctcgaga ctttcctcgg cgatcaagtc ccgcgctgtc cggtaatcga
     4381 cgccgaacat gtgttccccg gtggatacga ccgggtagcg actcttgacc agggccgaca
     4441 acggatcggg gtttgccccc ccctccacca cgggacgaaa aaagcccagc ttccggccat
     4501 gccgggccag catctccatc atccccagca cgaccgccga tttgccgctt cccggcccca
     4561 tccccgtgat gaaaaggttt ctggacattt ctcctctccg gcaatctcag cgttgagtgc
     4621 atccgccgtc gcaattcccc cggcccgtgg gatcgagatc tcttatcgtt ccaatatcac
     4681 gatcgaccgg gctcttaccc ggtactcgag gctggggacg agttgtcctt tgcccggttc
     4741 cgcaatgtcc tcggggctcg ggaggcccgt atccacgatt cggagccatt cgtccgcccg
     4801 gccctcctgc acggtaaatt ccaggtcctc ccaataggcg ttgatcatga cgtacagatc
     4861 tttgtccttg aggctctgtc cccccaaaca gtaagcgaga gtgcgggaat cccgggaaag
     4921 gtcgactccg ccggtcggcc cgtaccaccg aacgtctccg cgccagtagc gtccccgtcc
     4981 gatggacggt cgagccttcc ggaacgcgat catgagccgg aaaaaacgaa agaggtcccg
     5041 gttcttttcg agaaggtccc aatcgagcca ggtgatctcg ttgtcctggt tgtaggggtt
     5101 gttgtttcct ttctgggtgt tcatgaactc atcacccgca cagaacatgg gggtcccgtt
     5161 ggcgagcatg agcaggcaga agaagttttt gacctggcgt cttcgcagcg tcatgacttc
     5221 ctccgggacc cccgaatcgc cctcccagcc gcagttccag ctgaggttgt tgttggtccc
     5281 gtcggtgttg tcgtgcccgt tggcttcgtt atgcttttcg ttgtaggccg tcaggtcgta
     5341 gaggcagaac ccgtcatggg cggtgatgaa attcacgctc tggtacgggc ggtagacctc
     5401 caccggaccc tcggggaaga gatcgtcgct tccgtacagg cgttgcatca aggctcccac
     5461 cattccggga tcccctttca cgaaagcccg gacgtcgtcg cggaacttcc cgttccactg
     5521 ccgccacatg aggccgggga aacttcggcc gagcaggtag ctgttgatgt cccacgcttc
     5581 cgcaacgagt cgcacttccc ggaagtatcc gaaaaggctg atttcggcga tcaacggcgg
     5641 atccacggta ttcacggagc cgtccagggt gcgggtgaag atggacgcca ggtcgaaccg
     5701 gaacccgtcc acgcgcatgt cgatcgccca gtgctggaga ctgcacagaa cgagagcccg
     5761 aaccacggga tgggcgcagt tcagggtatt gccgcagccg gtgtcgttga agtattcgcc
     5821 ggaatcccgc cgggtcaggt agtagctccg gttgtcgatt ccccggtagc tgtacgtggg
     5881 accggtctcg tcgccttcgc tggtatggtt gtagaccacg tccagccaaa cctcgatccc
     5941 ggcctcgtga aatgccttca ccatcgagcg gaattccccg tcggggtccc ccgatgcata
     6001 accctggtgg ggggcaaaga agtttagggt catgtaaccc cagtagttgc cttcctgggg
     6061 gtcgaactgg tggatgggaa ggagttcgac cgcagtgatt cccaggtcct tgagatacgg
     6121 gatcttttcc gtgagcccgg cgaaagtccc tcgcttgtcc cggctcacgc ccgaattggc
     6181 ccgtgcggtg aagcccctga cgtggagttc gtagacgatg agatcatggg tgtgacgagg
     6241 aggaggggcc gtgccccagt cgaagggttt ttggccccgg gaaagccgcc ccaggggagc
     6301 ctttccgtcc gtgggtcccg gctccatggc tgccgaacga ctgtatcccg gcggaaaata
     6361 aaccgatgag gcaaagggat ccaggagcac cttttgggga tcgaaacggt gccctctcga
     6421 gggatcgtag ggcccgtcca cccggtatgc atagagagcg gcgccgcaca gctcctcttc
     6481 cggaacccga caatgccaca cgagtccggt ccggttgagg acgggatcca ggactcggga
     6541 atggaccggg ttggcagggt cccgctccgt gtagaaaagc agggtgacac tccgggcatg
     6601 gcgtgaaaac aaggcaaagt tgtaggcctt ctccgcctcc acccaactcg cccccggggg
     6661 ccagggggcg ccttccgttc gttcccattc atccgctgag gaaacaggtc ccgtcatgtt
     6721 cgctcctcga tccgccgatg catcccccca gaaggccggg gaccggttct gtcctccggg
     6781 tatccccgca ggatgtacgg aaagcccggt ggaaaaaaga ttccccctcg ggagtcccgg
     6841 cgaggacgca cgcgcgggtc ggcccccggg ttcgagccgg gggctccggg tgtttcgaat
     6901 gaatcctact tggcggtgca gagggtggtg cctacgcccc tcagggcaac gaccgcggcc
     6961 ttgacccgtt ccgccatggc cgcctcggcc gaaccgaggt aggaccgggg gtcgtaggtc
     7021 ttcttgtttc ccacttcccc gtccaccttg agcactccgt catagttgcg gaacatgtga
     7081 tcggccacgg cccgggtaaa ggcgtactgg gtgtccgtgt cgatgttcat cttgacgacg
     7141 ccgtagtcga tggcttcgtg aatctcctcg ggaagggaac cggagccccc gtggaatacg
     7201 agatagaacc gggcatcctt gccgtacaca ttctccacgg catcctggcc gtctttgaga
     7261 atggacggtt tgagcttgac gtggccgggt ttgtagaccc cgtggacgtt gccgaaggtg
     7321 gctgccagga gaaaccgtgc tcccgggacg gaacccaggc gccgggcgac ctccaccatg
     7381 tcctcggggg tcgtgtagag cttttcggcg ggggcgcctt cggccttgat tccatcctcc
     7441 tcgccgccaa ccaccccggc ctcgacctcg aggatcagct cgtttcgccg acagcgctcg
     7501 aggagaggaa cggcaatatc caggttttcc ttgaggggca gagcgctacc gtcaaacatg
     7561 tgactgttga aaaggttggg aagcccggcc ttgcgacgcc gttccgtttc ttccaccagg
     7621 ggcagaacga acttgtccag gacctggggc tgacaatgat ccgtgtgcag ggcgacgaaa
     7681 atgttgtaac gatccgccac cctgtggaca tgctcggcaa tggatatggc ccccagggcc
     7741 atgtccttca ccgaggaccc cgaggcgaaa gcccctcccc ccgtggaaac ctggaggatc
     7801 ccgtcgctcc ggctttcggc cagccctttg aggacggcgt tggcggtggt cagcgagctg
     7861 acgttgatgg cggggtaggc aaatcggttt tctttggctc gatcgagcat cttgcagtag
     7921 gtcttataat cggcaaccgg catgacatcc tcccgggcgt tgaaatttta ttggcttgcg
     7981 gggcttctcc cgggggaggg tcttagaatc ctccccgtcg gtggaggtag tcggccaggt
     8041 ccgccacccg gcaggaataa ccccactcgt tgtcgtacca g
//
LOCUS       DAOOVC010000165         7973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5478_length_7973_cov_5.830892, whole genome shotgun sequence.
ACCESSION   DAOOVC010000165 DAOOVC010000000
VERSION     DAOOVC010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_7973_cov_5.830892"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLM79_15555"
     CDS             complement(<1..361)
                     /locus_tag="PLM79_15555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87776.1"
                     /translation="MNSPKILVIDDEEIARISCERVLKREGIRVTLAAGGREGLDVLM
                     RDPHDLVLVDLKMPEMDGMEVTRRIRALDPNIVVIIITGYATIESAVAVMKEGAYDYL
                     PKPFTPDELLIVVRRGLE"
     gene            complement(456..872)
                     /locus_tag="PLM79_15560"
     CDS             complement(456..872)
                     /locus_tag="PLM79_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87777.1"
                     /translation="MASKGRILIVDDDPDFVKTTKMILLADGYEVSVATDGQQGLEMI
                     KVIEPDLVLLDIMMESIFEGFSFLGTLRTSPDYARLKATPILMISSVRADTGSRFSFG
                     DEEDMGDIPQPDEYMDKPLKPKDLLQKVASLLRRPA"
     gene            1102..2682
                     /locus_tag="PLM79_15565"
     CDS             1102..2682
                     /locus_tag="PLM79_15565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87778.1"
                     /translation="MWFQKLTWYRRIGTKISVLTSLLILLFMGAYTYLSIQNQRDQFL
                     EQIVRGVSLLSDTIKLSARYDMLLYSPEGLHRLVDTLGKQPSLEKIRIFNFLGEIIYS
                     SDKSEMHLQVDPRAEECYACHAAEKPLERLDTPERTRIFRTAAGDRVLGMINPVYNEP
                     DCYNASCHVHPAEQKVLGVLDIRVSLRNFDEKIAEAEAKILTLGLITLASLALLIKFL
                     IGRFLNNPLRALIEGTHRIAHGDLDSQIEIRSRDEIGIFAESFNRMTRDLKTAKESLT
                     EWGNRLEQMVGERTRDLEEAQKRLIRSEKLASLGKLAAGVAHEINNPLQGVLTFSELL
                     KERFPPDSSEFRDLNVIVQETIRCRNIVRGLLEFSRQSAPEKSSVDLHSLVEEVLRIL
                     ESQESFQNIRVTRNYDPQIPNIMADRDQIKQVFFNILVNASEAMDNAGELRIRTHWCP
                     ERSQVVIRFKDKGPGVEDDYLHQIFDPFFTTKPVGTGLGLAISYGIIKSHRGNIEVRS
                     KKGQGCEVVVSLPADVEN"
     gene            complement(2710..3300)
                     /locus_tag="PLM79_15570"
     CDS             complement(2710..3300)
                     /locus_tag="PLM79_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOV87779.1"
                     /translation="MKITRASDYPSKPSVLINGEMVVFKLMTREDCGALLSFFSRVPD
                     HEAEMLRHDVRDPAVICRWVDHLDYGEVFPLIAWNETLETITAVATLHYKKGIHRHVA
                     DIRVFVGQGYRKLGLGSAIIKELVDIGTRQGLYFLQAEVLTENQLALRAFKQLGFEYK
                     CTLEEYFMSRRKRTYDVALLLKRLRFNMEEDFFYLF"
     gene            3762..4577
                     /gene="proC"
                     /locus_tag="PLM79_15575"
     CDS             3762..4577
                     /gene="proC"
                     /locus_tag="PLM79_15575"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOV87780.1"
                     /translation="MFENVRIGFIGGGNMGEALIKGLLGSSLLKPDQVGLVDVDSARV
                     ELLKERHGIRGYHRMEDLARESRVILLAVKPQTVPDVLSRLSGQLVGRPLVISIAAGI
                     SLAQLEDALPDETPVIRVMPNNPALILQGASALSRGLYVTDEQMRMAVKLFESVGIAL
                     EVEEKCMDAVTGLSGSGPGYVFHLLEALIDAGVLVGLPRATSRELVLQTVKGAVLMAM
                     ETGKHPAELKEWITSPGGTTIRGLQVLEERGVHGAVMSAVQAATLRSQELGKK"
     gene            complement(4753..5499)
                     /locus_tag="PLM79_15580"
     CDS             complement(4753..5499)
                     /locus_tag="PLM79_15580"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87781.1"
                     /translation="MQGKRARHRPAIRLGGTLPRPSKEKMEDKPAGELLDVVLKCDSY
                     GSGEAVAGVLSKLGTPQAQIRVIHTGVGEISKTDLLLALSGSRLVLGFNVGVGPRLEQ
                     WAKEHDVEVRTYRLIYRLVEDIKGILQSLGRSRPEEKVTGKGKVIALFKTRPGGVILG
                     CEVTEGVLAQGSEFRIISAMGPVYRGRIQSLRIETRPVKEATRGQQVGLKIPDFDRAR
                     IGDGVECYQSLSTERPARWSPRGGVFPVDS"
     gene            5755..7452
                     /locus_tag="PLM79_15585"
     CDS             5755..7452
                     /locus_tag="PLM79_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOV87782.1"
                     /translation="MRPQRRKIVLLLGFYVLAYLVFLGERPLLRFDEARYAEIPREMI
                     ESGDWVVPRLNGVRYFEKPVLGYWLTALCLRVFGENNFALRLVPALCAGGTAWTVLWT
                     ASLGGAGISSLGVASAAVLLSSPLFFALGTLNLLDMPLTLFLTLCFAFFFAAYQSGRK
                     SGRVRFLALLGGAAGGAFLVKGFLGLVLPALGILPFLAWEHKLTSWVPRLWIPILTAC
                     LTALPWSLLIHWREPDFWNYFVWEEHWRRFVSEEAQHASPTWYFIPILLVGFFPWSLL
                     LPAALNGLKYAFRDNPLLRLSLCAFALPFLFFSFSSGKLSTYILPCFPPLAILTAAGL
                     LRALEAGEDRYFGKAVRGMIPVCVSAAAGLALVQVPGASFAPYLPEEAWKWVLGSASL
                     LFWAGLTWRAGKTRIPIRKILLVAACPLLAMILYQVAIPAAVEKEISPEEFLRAQKDR
                     VRQGEKLYAEKYLFHAVCWVYKRSDVILFDDPGELDYGLGYEDARDRMVSLKEFISIA
                     REGGEGKLLTLFTRKRIYLEVKDLLPPPVYEARDGGFVVSRYGSVPETTSRRSEAGF"
     gene            complement(7620..>7973)
                     /locus_tag="PLM79_15590"
     CDS             complement(7620..>7973)
                     /locus_tag="PLM79_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HOV87783.1"
                     /translation="LIKDNHVASLGGVAPAVQKAREKVPHTLKIEVEVETLEELVEAV
                     HAGADVVLLDNFSLEDLRKAVEINGGRCLLEASGGINLETVGAVARTGVDFVSCGALT
                     HSARAVDLTMEMITK"
BASE COUNT         1501 a         2471 c         2331 g         1670 t
ORIGIN      
        1 tctccaggcc ccggcgcacc acgatgagga gctcgtcggg tgtgaagggc ttgggaagat
       61 agtcgtaggc gccttctttc atgacggcca cggcgctctc gatggtggcg tatcccgtga
      121 tgatgatgac aacgatgttg ggatccaggg cccgaatgcg ccgggtaacc tccatgccgt
      181 ccatctcggg cattttcagg tccaccagga ccaggtcgtg gggatccctc atgagcacgt
      241 ccaggccctc ccgacctcca gccgccaggg tcacccggat cccctcgcgc ttgagcacgc
      301 gttcgcacga gattcgcgcg atttcttcgt cgtcaatcac caagatcttg ggagaattca
      361 cttttgtcct tgtcgagggg ccaaccttgc ctcgagactc ccttatgaac tcggatcgcc
      421 tttgggggcc ttgggatttc gtctgaaagc ggtctttacg caggccggcg gaggagactt
      481 gccaccttct gcagcaggtc cttgggtttg agaggcttgt ccatgtactc atccggttgg
      541 gggatgtctc ccatgtcttc ctcgtctcca aaggagaacc gggagcctgt atcggcccgg
      601 acgctggaaa tcatcagaat cggagtggct ttgaggcgtg cgtaatcggg ggaggtgcgg
      661 agggtgccca gaaagctgaa accttcgaaa atgctctcca tcatgatgtc gagcaaaacc
      721 aggtccggct cgatcacttt gatcatctcg agcccctgct gcccgtcggt ggctacgctg
      781 acttcataac cgtcggccaa aaggatcatt tttgtggtct ttacaaagtc gggatcatcg
      841 tccacaatca aaatcctgcc ctttgatgcc atgcatacac ctccgggggc tcatgaaaag
      901 ccttgcaaat tgcggttttt ttcaccattg cccagtaaat agcggaatcg cccttgcctg
      961 tcaactgcaa atctagtaac ttacttcaca aatgtttggg caaacgggaa aaaaagaaca
     1021 ttcgttccgg ggtctttccc gttcccggtg tgccggcgga aatccggatc ccgtttcacc
     1081 cccagagcga ggcccgagaa cgtgtggttt caaaaactga cctggtacag gcgtatcggc
     1141 accaagatca gcgtcctcac cagtctgctc atcctcctgt tcatgggggc ctacacctac
     1201 ctgtccatcc agaaccagcg ggaccagttc ctggaacaaa tcgtgcgggg cgtcagtctt
     1261 ctcagcgaca ccatcaagct cagcgctcga tacgatatgc tcctctactc ccccgaaggc
     1321 ctccaccgcc tggtggacac cctgggaaag cagccgtccc tggaaaagat ccgaatcttc
     1381 aattttctgg gtgaaatcat ctactcttcg gacaagtccg aaatgcacct ccaggtggac
     1441 cctcgggcgg aagagtgcta tgcctgtcac gcggcggaaa agcccctcga gcggctggac
     1501 acccccgagc gcacccgcat tttccgcacc gccgcggggg atcgagtcct ggggatgatc
     1561 aaccccgtct acaacgagcc cgactgttac aacgccagct gccacgttca tccggcggaa
     1621 cagaaggtcc tgggagtcct ggacatccgg gtatccctgc ggaactttga cgagaaaatt
     1681 gccgaggccg aggcgaaaat ccttaccctg ggcctcatca ccctggcttc cctggccctg
     1741 ctcatcaagt ttctcatcgg ccgctttctc aacaaccccc tccgggccct catcgaaggc
     1801 acccaccgca tcgcccacgg agacctggat tcccagatcg agattcgttc ccgggacgag
     1861 atcgggatct ttgccgaatc cttcaatcgc atgacccggg acctgaagac ggccaaggaa
     1921 tccctgaccg aatggggcaa ccgcctggaa cagatggtcg gcgagcgcac ccgagacctg
     1981 gaagaggccc aaaagcgact cattcgaagc gagaagctcg catccctggg aaagcttgcc
     2041 gccggtgtgg cccacgaaat caacaatcct ctccaggggg tcctcacctt cagcgagttg
     2101 ctaaaagagc gcttccctcc cgacagcagc gaattccgag acctcaacgt cattgtccag
     2161 gaaaccatcc gctgccgaaa catcgtccgg gggctcttgg agttttcccg acagagcgcc
     2221 ccggaaaaga gctccgtcga ccttcactcc ctggtcgagg aagtccttcg aatcctggaa
     2281 agccaggaat cgttccagaa catccgggtg accagaaact acgacccgca gattcccaac
     2341 atcatggcgg accgggacca gatcaaacag gtgttcttca atattcttgt gaacgcctcc
     2401 gaggccatgg acaatgccgg ggaactgcga atccgcactc actggtgccc tgaacggtcc
     2461 caggtggtca tccgtttcaa agacaagggt ccgggggtgg aagacgacta tctccaccag
     2521 atcttcgacc cgttcttcac caccaagccc gtcggcacgg gcctgggtct ggccatttcg
     2581 tacggcatta tcaagtccca tcggggaaat atcgaggtga gaagcaagaa ggggcagggt
     2641 tgcgaggtgg tggtttccct tcccgcggac gtcgaaaact gaaggtttcg cccctgcgga
     2701 cccctgcctt tagaagaggt agaagaaatc ctcctccatg ttgaagcgga gccgtttcaa
     2761 gagcaaggcc acgtcgtagg tgcgcttccg gcggctcatg aagtattcct cgagggtgca
     2821 tttgtattcg aaacccagct gtttgaatgc ccgaagggcc agctggtttt cggtgaggac
     2881 ctcggcctgg aggaaataca gcccctggcg ggtgccgatg tcgaccagtt ccttgatgat
     2941 ggcggacccc agccccaatt ttcgatatcc ctgccccaca aagacccgaa tgtccgccac
     3001 gtgcctgtgt atgccctttt tgtagtggag agtggccacc gccgttatgg tctcgagggt
     3061 ttcgttccag gcgatgaggg ggaagacttc cccgtagtcc aggtggtcca cccaacggca
     3121 aatgactgcc gggtcgcgaa cgtcgtggcg aagcatctcg gcctcatggt cgggaacccg
     3181 cgaaaagaaa gacagcagcg ctccgcagtc ttctcgagtc atgagcttga agaccaccat
     3241 ctccccgttg atcaggacgc tgggtttgga cgggtaatcg gaagcccgag tgatcttcat
     3301 cctagtggag acctccctcc atggccggcc tgggaagagg gcgaccgaag gcaccccttt
     3361 gggcccagtc ctcgaccgcc ggggatcggg atgccgaggc cggacctgtc tgccctttga
     3421 ggtccatatt accgggaagc ccggagggag acaaggcagt tccttcctca cgagagatcc
     3481 ttgacgaggg cccccctgtt cccaaaggaa atgaaggacg agctttccaa cccccgagga
     3541 gtcgcaagag acgaacctcg ccggcttccc tcgggaacaa gggaccggtg gtgccgacac
     3601 ggtgcggagg gcttgggcgg agacgggacc gggaagaggg aactatggat ttcgagtggc
     3661 agccggccac tcttctgtgg taaggagtcg gggacgacga gcgggtggag gctgctgccc
     3721 gtatcggcat ccacggaagg accgaacgct gggaggggat catgtttgaa aacgtgcgaa
     3781 tcgggttcat cgggggcggc aacatgggag aggccctcat caaggggctc ctgggatctt
     3841 ctctcctgaa accggaccag gtgggcctgg tggatgtgga ttcggcccgg gtcgaacttc
     3901 tcaaggaacg tcacggaatt cgcgggtacc accggatgga ggacctggcc cgggagagcc
     3961 gggtgatcct tctcgccgtc aagccccaaa ccgtcccgga cgttttgagc cggctgtccg
     4021 gtcaactcgt cggccgtcct ctggtcatct ccattgccgc cgggatctcc ctggcacagc
     4081 tggaggatgc gctgcccgac gagactcccg tcattcgcgt catgcccaac aaccccgctt
     4141 tgatcctcca gggtgcgtcc gccctcagcc gaggtctcta cgtgaccgac gagcagatgc
     4201 ggatggcggt gaagctcttc gagtccgtgg gcattgccct ggaggtggag gaaaagtgca
     4261 tggacgccgt cacggggttg agcggaagcg gtcccggtta tgtcttccac ctcctcgagg
     4321 cgctcatcga tgcaggggtc ctggtgggac tgccccgggc cacctcccgg gaactggtac
     4381 tccagaccgt gaagggggcc gtcctcatgg ccatggaaac cggaaaacat cccgccgaac
     4441 tcaaagagtg gatcacgtct ccgggaggca ccaccatccg cggcttacag gtcctcgaag
     4501 agcgcggtgt ccacggcgcc gtcatgagcg cggtgcaggc cgctactctg cgatcccagg
     4561 agttggggaa aaaataggcg cccggtccag ccaacagggc ttcttccacc gtcctcgggg
     4621 gcggaccttc ccttctgcct tcccgaaacg gcagaggtgg gtttcttctt ttgaagacgg
     4681 gcgcctccgt ccttgccctc cctcgacggg agggtctacg aaaggaggaa gaacccgtcc
     4741 tcggggcact cctcacgaat ccaccgggaa cacacccccc cggggactcc accgggcagg
     4801 tctttccgtg gaaagactct gatagcattc cactccatcc ccgattcttg cccggtcgaa
     4861 gtccgggatt ttcaaaccga cctgctgccc ccgggtcgct tccttgaccg gtcgcgtttc
     4921 gatgcgaagg gattggattc ttccgcggta taccggaccc atggccgaga tgatgcgaaa
     4981 ctcggaccct tgggccagca cgccttccgt gacttcgcac ccgaggatga ctcccccggg
     5041 tcgggtcttg aacagggcga tcaccttgcc cttcccggtg actttctcct ccggcctgga
     5101 ccgacccaag ctttgaagga tgcctttgat gtcttccacc agcctgtaga tcagcctgta
     5161 ggtcctcacc tcgacgtcat gctctttggc ccactgttcc aaccggggcc ccaccccgac
     5221 attgaatccc aggaccagcc tgctgccgct cagtgccagg agcagatccg ttttcgagat
     5281 ttccccaacg cccgtatgta tgacccggat ctgagcctgt ggggtgccga gttttgaaag
     5341 caccccggcc acggcttctc cgcttccgta ggaatcgcat ttcaatacca cgtccagcaa
     5401 ctccccggcc ggtttgtcct ccatcttctc tttggacgga cgcgggaggg tccctcccag
     5461 gcgtattgcg ggacgatgac gagcacgttt tccctgcatg gacctcaccc ccttgagacg
     5521 aaatcgcgcg gcgccccggc gcgccgacac acccaatata agctctcccc ggcgacgttt
     5581 cacgggaacg cgcccgttgc ccgaactcac atccgggaag ccgggttttt caacgggggc
     5641 ggattgtgat agaaaggcct ggccgtgcct ccatgggctt cttttgagac gcgcccgagg
     5701 gggacccgtc ggacgggcat gtcctttagg acttatggtg tcgggagatt gcgcttgaga
     5761 ccacaacgcc ggaaaatcgt gctgctgctc ggcttttatg tgctcgccta cctggtcttt
     5821 ctgggcgaac gcccccttct tcgtttcgat gaggcccgct atgccgagat tccccgggag
     5881 atgatcgaat ccggcgactg ggtggtgccc cgcctcaacg gagtgcgata tttcgaaaag
     5941 cccgtgctgg gctattggct caccgccttg tgtctccggg tcttcgggga gaacaacttc
     6001 gccctgcgcc tggttccggc cctgtgcgcc ggaggcaccg cgtggacggt tttgtggacg
     6061 gcttccctgg gcggggcggg gatttcctct ttgggcgtcg cctcggcagc cgtccttctc
     6121 agctctcccc tcttctttgc cctggggacc ctcaacctcc tggatatgcc ccttaccctg
     6181 tttctgacct tgtgtttcgc cttcttcttt gccgcctacc aatcgggtag gaagtccggc
     6241 cgagtccggt tcctggccct cctggggggg gccgcgggcg gtgccttcct ggtcaaggga
     6301 ttcctggggc tggtgctccc cgccctgggg attcttccgt tccttgcctg ggagcataag
     6361 ctcacctcgt gggttccccg cctctggatt ccgattttga ccgcatgcct gacggccctg
     6421 ccgtggtcgc tcctgatcca ctggagggag cccgactttt ggaactattt cgtctgggag
     6481 gaacactgga ggcggtttgt ttccgaagaa gctcagcacg cgagcccgac ttggtatttc
     6541 atcccgattc tcctggtcgg cttctttccc tggagccttt tgctgcccgc ggcgctgaac
     6601 ggtctcaagt acgcctttcg agacaatccg ctcttgaggc tttccctttg tgcttttgcc
     6661 ctccccttcc tgttcttctc attctcgtcg gggaagcttt ccacctacat cctcccgtgt
     6721 tttcctcctt tggcgatcct gacggctgcg gggttgctgc gagccctcga ggcgggagag
     6781 gaccggtatt tcggcaaggc tgttcgggga atgatcccgg tgtgcgtttc agccgccgca
     6841 ggactggccc tggtccaggt tccgggagcc tccttcgcac cgtatctccc cgaagaggcc
     6901 tggaaatggg tcctgggatc tgcaagtctg ctcttttggg cggggttgac gtggcgagcc
     6961 gggaagaccc ggattccaat ccggaaaatc ctccttgtgg ccgcatgccc actcttggcc
     7021 atgatcctct accaggtggc gatccctgcg gccgtcgaga aggaaatctc cccggaagaa
     7081 ttcctgaggg ctcagaaaga cagggttcgg caaggcgaga agctttacgc ggaaaagtac
     7141 ctgtttcacg ccgtctgctg ggtctacaag cgctccgacg tgattctttt cgacgatccg
     7201 ggagaactgg attacgggct gggatacgaa gatgcccggg accgcatggt gagcctgaaa
     7261 gagttcattt ccatagcccg ggaaggtggg gaaggaaagc ttctcaccct gttcacccgg
     7321 aaacgaatct acctcgaagt gaaggatctt cttccccccc cggtgtacga agcccgggac
     7381 ggcgggtttg ttgtgagccg gtacgggagc gtcccggaaa cgacatcccg gagatccgag
     7441 gcaggatttt agcggactcg ggacattagc ggcaacgccc cccctacaag ccggacccgg
     7501 ggacaaggct ggagatggga gctgcctgcc ggggtcttcc gccgtacttg gtaccggtcg
     7561 gcaagtatga aacggctcaa aagaggtgga aagggaagac tcgatgccgg ggtctcgtgc
     7621 tacttcgtga tcatttccat ggtgaggtcc accgcccggg ccgaatgagt cagggcaccg
     7681 cagctgacga agtccacccc cgttcgggcc acggccccca cggtttccag gttgatccct
     7741 cccgaggctt cgagaaggca ccttccccca ttgatttcca cggcttttcg caggtcttcc
     7801 agggagaagt tgtccaggag caccacgtcg gcaccggcat ggacggcttc caccagttcc
     7861 tccagggtct cgacctccac ctcgatcttc agcgtgtggg ggactttctc ccgtgccttt
     7921 tgcactgcag gggccactcc ccctaaactc gccacgtggt tgtccttgat gag
//
LOCUS       DAOOVC010000166         7961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5484_length_7961_cov_6.456615, whole genome shotgun sequence.
ACCESSION   DAOOVC010000166 DAOOVC010000000
VERSION     DAOOVC010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_7961_cov_6.456615"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..792
                     /locus_tag="PLM79_15595"
     CDS             547..792
                     /locus_tag="PLM79_15595"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOV87784.1"
                     /translation="MRTYNIHDAKTHLSRLVEQAANGEPFVIAKAGKPMVKVIPLNAR
                     EPSEIKRFGFMAGQMSVPADFDRMGETEIMKMFEEDA"
     gene            789..1238
                     /locus_tag="PLM79_15600"
     CDS             789..1238
                     /locus_tag="PLM79_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOV87785.1"
                     /translation="MKLLLDTHLLLWAAQGVEHLPVDARTVMSEPENELYFSVASLWE
                     IVIKCDLGRKDFKVDPRVLRRGLLDNGYLELPILSEHAVAVMTLPPIHKDPFDRLLVA
                     QAIVEGITLLTADIQLAKYAGPVQLVQQAGLAGAIFTKKGRNPPPPP"
     assembly_gap    1334..1433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1858..3600)
                     /locus_tag="PLM79_15605"
     CDS             complement(1858..3600)
                     /locus_tag="PLM79_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HOV87786.1"
                     /translation="MKSTPFLKDCLKTFRHDQDKARTPEETVRWARERLSRVDMDVLT
                     RTMRIDTGRLSIPVYISLCGKDSTRLTGTKKQMGKGATPAQSEASAVMELMERFSFFH
                     YIQNHPFPLATMESSAPHVLPVEELLGSIHDSSTPVAKCREFLAQCPLRWATAHNLTR
                     GENRRIPIDWFYLINEYNGPAAGNTREEAVLQSLCEVVERHVGTVISYGKVPTPLIAP
                     ESLEDPAAVELVGKFQRLGIRLYLRDFSLDTGIPTVGALAYDPSTFPGRSEIVFTAGT
                     TTSPEKSLCRALTEIAQLAGDFQNRTSYRPTLPKYETLEEAAFLLKDGEKVSIRSLPN
                     LEDDNFRVEIDRCVRALENKGWDVYTIDVTHPEIGVPAVYTVIPGAQFLDRTRDTDFA
                     QHAARTILRGLPQELVAPQMDRLLGLFGPRYDLTFFYAHSLERAEKPREALALFRKAL
                     HQHPDPSEIASIFVHVASCHKDLGEYREALNALETAEQHNRELKEIYNLRGFCHYRLK
                     EHQKAIAAFERAIEIDPGSAIDYANIGSNLRELGFREEAVRLYQMALELDPDIDFARE
                     NLARLTGEAEKRGR"
     gene            complement(3688..4581)
                     /locus_tag="PLM79_15610"
     CDS             complement(3688..4581)
                     /locus_tag="PLM79_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HOV87787.1"
                     /translation="MKPPLLVETSQKLSRIFPQLSGSQYIAVDTESNSFHAYFERLCL
                     IQVSTPEEDYILDPTVLEDLSPLGEILADPSIEKIFHAASNDVLGFKRDFRFHVRGLF
                     DTAIAGKLLGYKHLGLATFLREHFGVTLDKKWQRWNWAQRPLEEKQLDYARLDTHYLI
                     PLRHRLAARLREQNLWDAARDAFQKACEQTFQEKVFRPGGYLHLRGARLLDADGKRVL
                     KALYLYREQEAKRRDIAPFRILSNETLVRLAQSRPTDLEELVRVKGMPKVYRHPSSAQ
                     QLLQRIRKAESGDGGVPLAHR"
     gene            4864..5409
                     /locus_tag="PLM79_15615"
     CDS             4864..5409
                     /locus_tag="PLM79_15615"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOV87788.1"
                     /translation="MAGVVPMDRTRGTRIPPVPPAGNPPRFLAREDLEVYTYLVDLVT
                     GEAAFELTLSDEYIDGALVGLSPDILRKLRKGEFSYQDYIDLHGYKREQARERVTRFL
                     LESFAKKLRCVLVVSGRGLNSRDKQPVLKKGLVSWLTRAPLKRVVLAFASARSHDGGA
                     GAFYVLLRRNSGKGPVVSPAP"
     gene            complement(5649..6110)
                     /locus_tag="PLM79_15620"
     CDS             complement(5649..6110)
                     /locus_tag="PLM79_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV87789.1"
                     /translation="MTAVSCSSVSEVVRFAIEKEEKAMDFYQKCADRAKNPGIKQFFM
                     EMVQEEQRHRDLLKDLNPATLEGVQLSKPEDLRISDYLVDVKFRDDLTYQEALTIAMK
                     KEEKAYAFYTGWQNKCMHEKTAKLFELLAAEEMKHKRKIETVYDEEILTWD"
     gene            complement(6161..7891)
                     /gene="ade"
                     /locus_tag="PLM79_15625"
     CDS             complement(6161..7891)
                     /gene="ade"
                     /locus_tag="PLM79_15625"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HOV87790.1"
                     /translation="MKWNSERLARVIRVAQGKQPAEMVLRRCRLVNVLTGKVEEADLA
                     IQDGIFVGWGPYEGEKVLDLEGKYVCPGFIDGHIHLESSLLSPAQFCAAAVPWGTSAV
                     VADPHEIANVLGLDGIRYFLDAAEGLPLDIYFNLPSCVPATHIETSGAQLRAADLHSL
                     LPHPRLLGLAEMMNFPGVLLAVPEVLDKLILFQDRGMDGHAPQLAGLGLNAYLATGVG
                     SDHECTVLEEAREKLGKGMAIMIREGSQSKDLAALLPLVDGSTWPRCMLVSDDRHPDH
                     LARDGHMNVSVNLAMELGMDPVRAIALATWTPARHFGLTRRGAVAPGYQADFSVSPTL
                     NPWKPERVFKKGAEVARNGKLTDFSGFSRTATVPPSPMRISRLEAADLEVAAEPGLLR
                     VIAVREGTLLTGEMRVPPRIVDGKAVADTDRDILKLVVFNRYIPDRPPSAAFVHGIGL
                     KKGAVATSVAHDSHNVIAAGAGDEDIVHVVSALREAGGGMAIGEAGSPVEILPLPVAG
                     LMSDQPLEFVVDRLKSLKHRAGKWGCSLNDPFMALSFLALPVIPSLKLTDLGLVDVNA
                     FSFVPLFKTD"
BASE COUNT         1534 a         2334 c         2363 g         1630 t
ORIGIN      
        1 cctgatttga gcgattccga gaccatcccc tcgtcatgcc cacgaaggcc ggcacccagc
       61 gggtatgcac gcttttcagg gattcccgtt tgtccgcagg aatgaggaaa cgggatgcgt
      121 gccggccaaa tcgctcaacc caggacagag tccgacattt gcgggagtat gcggaaacct
      181 caaaaacggt gaactgctcg gagtttacag aaagggaagc ttccgtatgg atgagtatca
      241 aagtttaagc cgcaccctat gaatacgccg tggacaaatc cggtgtgctt cctgatccac
      301 cccagcccca ccccctcctc ggtggtctca accgtgaaag agccctccga cagggcggta
      361 tggccgcgcc acccttcatt tcgccttgaa cctgcccgat tgacttgatg atggcgttca
      421 ccttcccgag aaagatatgt tgccaccgtt tgattgcgga gaaatgaggc tggttcttac
      481 cccttgacat ggcgctggat gacgataaac taagtttatc ggactaagtc ttattgaggt
      541 ggcccgatgc ggacatacaa cattcatgat gccaaaaccc atttatctcg tctggtggag
      601 caggccgcca acggtgagcc gttcgtcatc gccaaagccg gcaagccaat ggtcaaggtt
      661 attccgctca acgcgcggga gccttccgaa atcaaacggt tcggctttat ggctggacaa
      721 atgagcgtgc cggctgactt tgaccggatg ggcgaaaccg agatcatgaa gatgtttgaa
      781 gaggacgcat gaagttgttg ctcgacactc atctgttact atgggcggca caaggcgttg
      841 aacatctacc ggtggatgct cggaccgtga tgagcgagcc tgaaaacgaa ttgtatttca
      901 gtgtcgccag cttgtgggaa atcgtaatca aatgtgactt gggccgcaaa gatttcaaag
      961 tcgatccacg agtgttgcga cgcggcctgt tggacaacgg ctacctggaa ctgccgatcc
     1021 tcagtgagca tgcggtggcc gtcatgaccc taccgccaat ccataaagat cccttcgacc
     1081 gcctgctcgt cgcgcaggcc attgtcgagg gtataacgct actgacggcc gatatacagt
     1141 tggccaaata cgccggtccg gtgcaactcg tccagcaagc ggggctggcc ggggcaattt
     1201 tcaccaagaa aggccgcaac ccgccccctc ctccataata ggggatcatg gaagggccgg
     1261 accacactcg gaatccgtgg tgtggaattc cgtactgata cctcccaaat ggttcgatcc
     1321 tcgaatattt ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgtgccg
     1441 atggggattg ggtcctttga agttcctgat aaaggcatgg agaccgccac gttggcatcg
     1501 gcggacccct cttggtgaga ttgaaacgat ggtcgtgaaa gcgccaccat caaaaccctc
     1561 gagtaacccg gcgtaataca tctccagaag cggcctgaag agaacttgag ttgaggaggt
     1621 gggagttgag cggtgtggaa atacctgccc gaaccttcca cccggtctcc cgcactcggg
     1681 acctccggag catagcgaag ggcagccgct ccctcttttg ccccttgact atgggggcta
     1741 gtcatgggat acgccgcgga caaacccggg tgtgctttcg gacctacacc cagcccgatt
     1801 cgtcccgggt ggtttcagcc aaccaacaac ccgcccgagg tccccggcaa gctcggttta
     1861 cctcccccgt ttttccgcct cccccgtcaa gcgggcaaga ttttcccggg cgaaatcaat
     1921 gtcggggtcc agctcgaggg ccatttgata gagacgaacg gcttcttctc gaaatcccag
     1981 ttccctcagg ttggacccga tgttggcgta gtcgatggcg gagccgggat cgatttcgat
     2041 ggcccgttcg aaggccgcga tggccttttg atgctccttc aggcgatagt ggcaaaagcc
     2101 tcgaaggttg tagatctcct tcaactcacg gttgtgctgt tcggccgtct cgagggcgtt
     2161 caaggcctcc cggtattccc ccaggtcctt gtgacaggat gccacgtgca caaagatgct
     2221 ggcgatctcc gaggggtccg ggtgttggtg cagggccttg cggaacaggg ccagggcttc
     2281 ccggggcttc tccgcccgtt cgaggctgtg ggcatagaaa aaggtaagat cgtaccgggg
     2341 accgaaaagc cccaacaggc gatccatctg aggggccacc agttcctggg gaaggccccg
     2401 caggatcgtt cgggccgcat gctgggcaaa atccgtgtcc cgcgtgcgat ccaggaactg
     2461 ggcccccggg atcacggtgt acaccgccgg gaccccgatc tcgggatggg tcacgtcgat
     2521 ggtgtagacg tcccatccct tgttctcgag agcgcggacg catcggtcga tctccacccg
     2581 gaagttgtcg tcctccaggt tggggagact ccggatggaa accttctccc catccttcaa
     2641 gaggaaggcc gcctcctcga gggtttcgta cttgggaagg gtagggcggt aggaggttcg
     2701 gttctggaaa tctcccgcca gttgcgcgat ttcggtcagt gccctgcaaa gagacttttc
     2761 cgggctggtg gtggttccgg cggtaaagac gatctcgctg cgtccgggaa aggtcgacgg
     2821 atcgtaggcc agggccccca ccgtgggaat tcccgtgtcc agggagaaat cgcgaagata
     2881 gagccggatt cccagccgct ggaattttcc caccagttcg acggccgccg ggtcctcgag
     2941 ggactcgggg gcaatgaggg gggtggggac tttcccatag ctgatgacgg ttcccacgtg
     3001 ccgttccacc acttcgcaca agctttgcaa aacggcttcc tcccgcgtgt ttcccgccgc
     3061 cggtccgttg tattcgttga tcaggtagaa ccagtcgatg ggaatccgcc ggttttcccc
     3121 ccgagtcagg ttatgggccg tcgcccacct caaaggacat tgcgccagaa actcccggca
     3181 ttttgcgacg ggggtggaag agtcgtgaat ggagcccagg agttcctcga cgggcaaaac
     3241 gtgaggggcc gaagattcca tggttgcgag gggaaacgga tggttttgaa tatagtggaa
     3301 gaagctgaag cgttccatga gctccatcac ggcgctggct tcggactggg cgggggtggc
     3361 tcctttcccc atctgcttct tggtccccgt gagacgggtg gagtccttgc cgcacaggct
     3421 gatgtacacg gggatggaaa gcctccccgt atcgattcgc atcgttcggg tcagcacatc
     3481 catgtccacc ctcgagaggc gttcccttgc ccatcggacc gtttcttccg gggttcgggc
     3541 cttgtcctgg tcgtgtcgaa aggtcttgag gcaatccttg agaaaagggg ttgatttcat
     3601 ggatgaacct tctggtgatg gtggtactgg aaaacgttct ttgggggttc ttcccccgga
     3661 ggctggaacc tcggcgaatt cggcgaatca acggtgggcg aggggcactc ctccatcgcc
     3721 gctttccgcc ttccgaatgc gttgcagcaa ttgttgggcg gacgaaggat gccggtagac
     3781 cttgggcatg cccttgaccc ggacgagctc ctcgaggtcg gtgggccggg attgggcgag
     3841 gcgcaccagg gtttcgttgg aaagaatccg gaacggggcg atgtcgcgcc ttttggcttc
     3901 ctgttcccga tagagataca aggccttcaa gactcttttc ccgtccgcat cgagcagccg
     3961 agcccctcga aggtgcaggt atccgccggg acgaaacacc ttttcctgga aggtctgttc
     4021 gcaggctttt tggaaggcat cccgagccgc atcccagagg ttctgctccc ggagtcgggc
     4081 ggcgagccgg tgtctcaagg gaatgaggta atgggtgtcc aggcgggcgt aatccagctg
     4141 cttctcctcg aggggccttt gagcccagtt ccagcgctgc catttcttgt ccagggtcac
     4201 tccgaaatgt tcccggagaa aggttgccag gcccaaatgc ttgtagccca agagcttccc
     4261 ggcgatggcc gtatcgaaca ggccccgcac gtggaatcgg aagtcacgct tgaaccccag
     4321 cacgtcgttg gaagccgcat gaaagatctt ctcgatgctt ggatcggcga ggatttcccc
     4381 caggggactg aggtcctcca agacggtcgg gtccaggata tagtcctctt cgggggtgga
     4441 gacctgtatg aggcacagcc gttcgaagta ggcgtggaag ctgttggatt ccgtatcgac
     4501 ggcaatgtat tgcgatccgg aaagctgcgg aaatatccgg gagagcttct gggatgtctc
     4561 tacgagaagc ggtggtttca tgaaacggtt tcctgtttcc ggctcctatg gtatgaatag
     4621 acggccccgg gacgaggtaa cagcatggcc tgcaagagaa aacgaccctc cctctccaaa
     4681 cgtcgacctc ccagcccaaa acccgctgaa tcggttttct ataccccctt tgtaggatta
     4741 gatcaacagc tgtcgagaaa atcccctcct tcgcctccaa cttccgcaag tcccccggcg
     4801 ggggacgaac cccggccgtc ggagcctccc gaacgggagc aggagaagct tttccgcgaa
     4861 gccatggccg gtgtcgtgcc catggatcgg acccggggaa ctcgtattcc ccctgtcccg
     4921 cccgccggga atcctccccg tttcctggcc cgggaagacc tggaagtata cacgtacctg
     4981 gtggacctcg tcaccgggga agccgccttc gagctcaccc tttccgacga gtacatcgac
     5041 ggtgccctgg tggggctttc ccccgatatc cttcgtaagc ttcgcaaggg agagttcagt
     5101 taccaggatt acatcgatct gcatgggtac aagcgggaac aggcccggga gcgggttacc
     5161 cgcttcctgc tggaaagttt tgccaagaaa cttcggtgcg tcctggtggt ttccggccga
     5221 ggtttgaact cccgggacaa gcaacccgtc ttgaaaaagg gcctggtgtc ctggctgact
     5281 cgagcccccc tcaagcgcgt agtcctggcc ttcgcgtcgg cccgatccca cgacggcggg
     5341 gcaggggcgt tctatgtgct cttgcgccgc aactcgggaa aaggacctgt cgtctctccg
     5401 gccccgtagg gggcgtctgc cccgcgcaca agggtttaat tcagtaggaa tccctattct
     5461 tgcgtccggc cccgcgtctg ccctgcgtgc aagggtttgg ttcctcgaag gggttcggga
     5521 ccggcctgcg tcaccggtca agaagggtgg gaaagtctgt tcccctcgcc ccgaccgccg
     5581 gagagggcac ctttggcccg ggcatgggcg ggcagggcag gggggatgcg aatcttcggg
     5641 aaggcgggct aatcccaggt caggatctct tcgtcgtaga ccgtctcgat tttccgcttg
     5701 tgcttcatct cctccgccgc caggagttcg aagagctttg ccgtcttttc atgcatgcac
     5761 ttgttctgcc acccggtgta gaaggcgtag gccttttcct cttttttcat ggcgatggtc
     5821 aaggcttcct ggtacgtgag gtcatcccgg aatttgacgt ccaccaggta gtcgctgatg
     5881 cgcaggtcct cgggcttgga aagctgcacg ccctcgaggg tagccgggtt caagtccttg
     5941 agcaggtctc gatgccgttg ctcttcttgg accatttcca tgaagaactg cttgatgccg
     6001 gggtttttcg cccggtcggc acacttctgg taaaaatcca tggccttttc ttctttttca
     6061 atggcgaagc ggacgacttc actcacggaa ctgcacgaaa cggctgtcat cgttgcctcc
     6121 tatcggtctg ctgatgattg agtcctgtgg gaggatccca ctaatctgtt ttgaacaggg
     6181 gaacaaacga aaacgcattc acgtccacca ggccgagatc cgtcaatttc agggagggga
     6241 tcaccggcag ggcgagaaaa gacagggcca tgaacggatc gttcaaagag catccccatt
     6301 tcccggcccg gtgtttgagg gacttgagcc ggtccaccac gaattccaag ggctgatcgg
     6361 acatgagacc cgccacgggc aacggcagga tctcgaccgg ggaccccgcc tccccgatgg
     6421 ccatgccccc tccggcctcg cgtagggccg acaccacgtg cacaatgtcc tcatccccgg
     6481 cgcccgccgc aatcacgttg tgagaatcat gggccacgct cgtggcgaca gcccctttct
     6541 tcaggccgat tccgtgaaca aaagccgcgg acggcggccg atcgggtatg tagcggttga
     6601 agaccacgag cttgagaata tcccggtccg tgtcggcaac ggcttttccg tccacgatcc
     6661 ggggggggac ccgcatttcc ccggtgagca gagtcccctc ccgaaccgcg atcacccgaa
     6721 gcaaacccgg ctccgctgcg acttccaggt ccgccgcttc gaggcgggag attctcatgg
     6781 gactcggggg gaccgttgct gtccgcgaaa atcccgagaa gtccgtgagc tttccgttac
     6841 gggcaacctc ggctcccttc ttaaagaccc gctcgggctt ccaaggattt aacgtcgggc
     6901 tcacgctgaa atcggcctgg tagccgggag ccacggcccc ccggcgggtc aggccgaaat
     6961 gccgggcggg agtccacgtt gccagggcga tggcccgaac cgggtccatt ccgagctcca
     7021 tggccaggtt caccgagacg ttcatgtgac cgtcccgggc caggtgatcc gggtgccggt
     7081 cgtcgctcac cagcatgcag cgcggccacg tggaaccgtc gaccaggggc aagagagcgg
     7141 ccaggtcctt ggactggctt ccctcccgaa tcatgatggc catcccttta cccagttttt
     7201 cccgggcttc ctcgaggacc gtgcactcgt ggtccgatcc gactcccgtg gcaagataag
     7261 cattgagtcc caggcccgcc agttgaggtg catgcccgtc catcccgcga tcctggaaca
     7321 ggataagctt atccaggact tccggcacgg ccaaaaggac gccgggaaaa ttcatcattt
     7381 cggccaggcc caggagtcgc gggtgaggca gaagggaatg aaggtcggcg gcccgaagct
     7441 gcgccccgga ggtctcgatg tgggttgcgg gaacacagct ggggaggttg aaatagatat
     7501 cgagcggaag cccctctgcg gcatcgagga agtatcgaat gccgtccagg cccaggacgt
     7561 tggcgatttc atgggggtcc gccaccacgg ccgaggtgcc ccacggcacg gccgcagcac
     7621 aaaactgggc cggactcaaa agggagctct ccaggtgaat gtgaccgtcg ataaagcccg
     7681 ggcacacgta cttgccctcg aggtccagga ccttctcccc ttcatagggt ccccagccca
     7741 caaagatccc gtcctgaatc gccaggtcgg cctcttccac cttgccggtg agcacgttca
     7801 ccagtcggca acgacgcaag accatctcgg cgggctgttt tccctgggca acccggatca
     7861 cccgggccag tcgctcactg ttccatttca tcggttctta tcctcccacc ccctgcaaac
     7921 ccggcgtccg cccttacgac aagtccgagg gcgccgtccc g
//
LOCUS       DAOOVC010000167         7937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5505_length_7937_cov_7.354986, whole genome shotgun sequence.
ACCESSION   DAOOVC010000167 DAOOVC010000000
VERSION     DAOOVC010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7937
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_7937_cov_7.354986"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PLM79_15630"
     CDS             <1..504
                     /locus_tag="PLM79_15630"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87791.1"
                     /translation="SRGRRVDLTRCIGEVAASRRRVQVLSRLQGFQVKLWGDEGWKGL
                     LPESVTYCGPAGHYRELTRIYNATSVNLDINRIYQKDIVTMRVFDVVACRGFVLADHS
                     DALADLFHPGTEIVSYRTLDELPRLVEYFLLRPDQREAVAAAGYERLLKEHTIRKRLQ
                     QMLSRLP"
     gene            517..2322
                     /locus_tag="PLM79_15635"
     CDS             517..2322
                     /locus_tag="PLM79_15635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87792.1"
                     /translation="MNANPTVSLCMIVKDEEANLVACIESVRTLVDEIVVVDTGSRDG
                     TVELARALGARVFSFAWNRDFSAARNESLRHARGEWVLYLDADERLNPDGMGDCLRRA
                     AAVPGVDAYSTPIKNRRNHRGQVYFQVVYNVRMFRRYPGIRFSGEVHERVEPFLEKVN
                     ANIAQAGFFIEHLGYDIERAEEVKKLERNLDLTRKALERDPDDGYALYYLGLTLLGLQ
                     RMEESREALENALEARGLTPNLKALILNLLSYHHILNHCYSQAVRRATESLGSVPVQN
                     TARLFLGFALFQQNDYPNALPHLLRVNEFLRLPPEKRTTRLSQEHSIQEADLWKMIGV
                     CFLQTQDFPRALEYLRKYAIERAQDAEAHRLVGVCALNCQEFDLALDHLTTAKRLGIP
                     ADSLSLPLAYAHLRAGDPSRALRYFEASRETTEEEIAASLQILECFARSPDAMPLLPS
                     LLEKKRSVLARASRGLLDRLLGALADARNYDALVRVLEGIPAEGDDFEALLRGIEVRF
                     GAQGRHGDWVRFLEEFVRARPQNPTVGCLLGTARIRAGDFPKAIEAFTRVRGQCPPEA
                     GVDRILAGLFLKVGDPEKARYHLALSRTAASSAGK"
     gene            2349..3992
                     /locus_tag="PLM79_15640"
     CDS             2349..3992
                     /locus_tag="PLM79_15640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87793.1"
                     /translation="MTTSRFALSVCMIVKNEAHQLGEALDNFADFADELVVVDTGSRD
                     DTQVVASRFTPYVYHFPWCDDFSAARNFSLEKARGRYVLWLDADDRFEPAMQGCLREL
                     KKHFSTGRAFYFILENVDETGPSWSCYQLRCVPNRPDVRFEGRVHEQLYPSVERAGIE
                     GVLTDIVVRHYGYRERGKFLEKTRRNLAILDKERLEGRDDEHVHYYRALAQEALGLFP
                     QAVSSMERALVHLERRIHQRFGDNPSPDLQSVLEGYLFLARNYHRLGEIPSARRNLLR
                     ANALADEDSNVSYRLGCMFQKLGLHLQALECFGKALSTGTRRVRFHPSDRLPPPATLH
                     LHRAFSLFCMNRETEGLRALEEVSSRGISPSEMFEWLGLRALQGGEYQLSLQFYRAAR
                     QRGELSSDGLCNLGLIQAKLGDVCGALECYDAAIEKTPGHPAALANRAHLHFRMNEWD
                     KAEKEFEALVRDRHRDLDLLLPLALISLKKGNRNGFARAERLIFDQVSPPPAVRSSED
                     RFRWLGDVLDAERKGMPAVWARQIARELGPDAPPGSHFL"
     gene            4288..5136
                     /locus_tag="PLM79_15645"
     CDS             4288..5136
                     /locus_tag="PLM79_15645"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOV87794.1"
                     /translation="MGLRINTNIEAMEIHRQLVLTNNKLGDSLKRISSGYRINSAKDD
                     ASGFAVANMFKAKISSMRVAYQNGSEANSMLQVADGAYNKIYDVLVRMKDLATQAASG
                     QTENRDKLQVEFAELQAEIDRIAQSTTYSTKNAASLQLINGSSDISGGITFMVGATNN
                     SQNMIGITLSAACTGALGVGSNLLSIDSVGSARAAMDALDLALGSINTYMGKVGSYQN
                     RLQITMENLSTGIENFSASESTIRDVDMAYEVMNFTREQILQQSGMAMLAQANTAPQQ
                     VLTLLR"
     gene            5438..6286
                     /locus_tag="PLM79_15650"
     CDS             5438..6286
                     /locus_tag="PLM79_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOV87795.1"
                     /translation="MGLRINTNIEAITIHRQLVETSSKLGDSLRKISSGYRINSAKDD
                     ASGFAVANMFKAKISSMRVAYQNGSEANSMLQVADGAYNKIYDVLVRMKDLATQAASG
                     QTENRDKLQVEFAELQAEIDRIAQSTTYSTKNAASLQLINGSSDISGGITFMVGATNN
                     SQNMIGITLSAACTGALGVGSNLLSIDSVGSARAAMDALDLALGSINTYMGKVGSYQN
                     RLQITMENLNTGIENFSASESTIRDVDMAFEVMNFTREQILQQSGMSMLAQANTAPQQ
                     VLTLLR"
     gene            6891..7343
                     /locus_tag="PLM79_15655"
     CDS             6891..7343
                     /locus_tag="PLM79_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87796.1"
                     /translation="MGWRFRVIQAWVLWICVLLPLKVDAVTTTYGGETLEWEVRAGGK
                     PLDPAFQGCEDTSWGVECNYGGVFPSVGEQLSLAAIISPSQWWDGYNCLQMGTMDTES
                     ITGCECKPLMLSGTTLFYPQSLKYSVQIVYMRAQVSPIGRNYTFKVNL"
     gene            7421..7909
                     /locus_tag="PLM79_15660"
                     /pseudo
     CDS             7421..7909
                     /locus_tag="PLM79_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1667 a         2441 c         2337 g         1492 t
ORIGIN      
        1 tcccggggac gaagggtgga cctgacccgt tgcatcgggg aggttgcggc ctcccggcga
       61 cgagtccagg tgctctcgag gctccaagga ttccaggtga agttgtgggg ggacgaaggg
      121 tggaaggggc tccttcccga aagcgtcacc tactgcgggc ccgccggaca ttaccgggag
      181 ctgacccgaa tctacaatgc cacgtccgtc aatctggaca tcaaccgcat ctaccagaaa
      241 gacatcgtga ccatgagggt cttcgacgtc gtcgcctgcc gaggttttgt cctggcggac
      301 catagcgacg ccctcgcgga tcttttccac ccgggaacgg aaatcgtctc gtaccgcacc
      361 ttggacgaat tgccccgtct cgtggaatac ttcctcctcc gtcccgacca gcgggaagcc
      421 gtggcggctg cgggatacga gcgattgttg aaggaacaca cgattcggaa gcgactgcaa
      481 caaatgttga gccgtctgcc gtaggaccgg tccaccatga acgccaatcc caccgtttcc
      541 ctgtgcatga tcgtcaagga cgaggaggcc aacctggttg catgcatcga gagcgtgcgg
      601 accttggtgg acgagatcgt cgtcgtggac acgggttccc gggacgggac cgtcgagctg
      661 gcccgggcgt tgggggccag ggtcttttcc tttgcgtgga accgggattt ctcggcggct
      721 cgcaacgaat ccctgcgcca cgctcggggg gaatgggtcc tctatctgga tgccgacgag
      781 aggctcaacc ccgacggaat gggggattgc ctgcgcaggg ccgcggccgt tcccggcgtc
      841 gacgcctact cgacgcccat caagaaccgg aggaatcacc gggggcaggt ctactttcag
      901 gtggtctaca acgttcggat gttccgacgc tacccgggga tccgattttc gggggaagtg
      961 cacgagcggg tcgagccctt tctcgaaaag gtgaacgcca acatcgccca ggcgggcttc
     1021 tttatcgaac acctgggcta cgacatagaa cgagccgagg aggtgaagaa gctcgagcgg
     1081 aacctggatt taacccgaaa agccctggaa agggaccccg acgacgggta cgccctgtac
     1141 tacctgggtc tcaccctcct ggggctccaa aggatggagg aaagccggga ggccctcgag
     1201 aacgccctcg aggcccgggg gctgactccc aatttgaagg ccctcatcct gaacctgctg
     1261 agctaccatc acattctgaa tcactgctat tcccaggcgg ttcgacgggc aacggaatcc
     1321 ctgggctccg ttcccgtcca aaacacggcc cggcttttcc tgggatttgc cctgtttcag
     1381 caaaacgatt acccgaacgc cctgcctcat ctgctccggg taaacgagtt tctccgtctt
     1441 cccccggaaa aacgaaccac ccggttgagc caggagcact ccatccaaga ggcggacctg
     1501 tggaaaatga tcggggtttg cttcctgcag acgcaggatt ttccccgtgc cctcgaatac
     1561 cttcgaaaat atgcgatcga acgggcgcag gatgccgagg cccatcggct cgtgggtgtc
     1621 tgcgccctga actgtcaaga gtttgaccta gcccttgacc acctgacgac ggcaaaacga
     1681 ttgggtatcc cggccgattc cctttccctg cccctggcct acgcccacct gcgggcggga
     1741 gacccgtccc gggccctgcg gtattttgag gcttcccggg agaccactga ggaggagatt
     1801 gcggcttccc tgcagatcct cgagtgcttt gcccggagtc cggacgccat gccgttgctg
     1861 ccgtccctcc tcgagaagaa acgatccgtc cttgcccggg cttcccgggg cttgctggat
     1921 cgcctgctgg gcgcccttgc cgacgctcga aactacgatg ccctggtgcg ggtcctcgag
     1981 gggatcccgg cggagggaga cgatttcgag gctcttctcc gagggatcga agtccgcttt
     2041 ggagcacaag gcaggcacgg agattgggtc cgattcctcg aggagtttgt ccgtgcacgt
     2101 ccccaaaacc cgaccgtcgg gtgcctcctg ggaaccgcac ggattcgagc gggggatttc
     2161 cccaaggcca tcgaagcgtt cacccgggtg cggggccagt gtcctcccga ggcgggagtg
     2221 gaccgaatcc ttgcgggcct gttcctgaag gtgggagacc cggagaaggc acggtaccac
     2281 ctggcccttt cgcgaactgc cgcgtcttcg gcgggcaaat gaagcccccg gctacgggaa
     2341 tccagggcat gacgacctct cgattcgctc tatccgtctg catgatcgtg aaaaacgagg
     2401 cccaccagtt gggggaagcc ctggacaatt tcgccgattt tgccgacgag ctcgtagtgg
     2461 tggacacggg ttcccgggat gacacccaag tcgtggcttc ccgcttcacc ccctacgtct
     2521 accatttccc ctggtgcgac gacttttccg ccgcccgcaa cttttccctc gagaaggctc
     2581 ggggacgtta tgtcctgtgg ctggatgccg acgatcgatt cgaacccgcc atgcagggat
     2641 gcctccggga actaaagaag catttctcca cggggcgggc cttctatttc attctcgaga
     2701 atgtggatga aacgggacct tcctggtcct gctaccagct ccgttgcgtt cccaaccggc
     2761 cggacgttcg cttcgaggga agggtccacg aacagcttta tcccagcgtg gagagggccg
     2821 gaatcgaagg ggtcctaacg gacatcgtcg ttcgccacta cgggtaccgg gaacgcggca
     2881 agttcctgga gaagacccgg cgcaacctgg cgatcctgga caaagaacga ctcgagggcc
     2941 gagacgacga gcacgttcac tactatcggg ccctggctca ggaggcgctg ggccttttcc
     3001 cgcaggccgt ctcctccatg gaacgtgccc tggtgcacct ggaacggcgg atccaccagc
     3061 gcttcgggga taacccgtcc cccgacctcc agtcggtcct cgagggatat ctttttcttg
     3121 cccgcaatta ccaccgactg ggggagatcc catcggcccg tcgaaacctc ctccgggcca
     3181 acgccctggc cgatgaagac tcgaacgtgt cgtaccgcct gggctgcatg tttcaaaaac
     3241 tggggctcca tctccaggcc ctcgaatgct tcgggaaggc tctatccacc gggacccgga
     3301 gggtacgatt ccacccgtcg gaccggttgc ctcccccggc aaccctccac ctgcaccggg
     3361 ctttcagtct cttttgcatg aaccgggaga cggagggcct ccgggccctc gaagaggtga
     3421 gcagccgcgg gatttctccg agcgaaatgt tcgagtggct gggtctaagg gcgctccagg
     3481 gcggggagta ccagctttct ctgcagttct accgagcggc acgacaacgg ggggaactga
     3541 gctcggacgg cctgtgcaac ctgggcttga tccaggccaa gctgggagac gtgtgcgggg
     3601 cgctcgagtg ctacgatgcg gccatcgaga agactcccgg ccatcccgcc gccctggcca
     3661 accgcgctca cctgcatttc cgtatgaacg agtgggacaa ggccgagaaa gagttcgaag
     3721 cccttgtgcg ggaccggcat cgggacctgg acctgctgct gcccctggct ctcatttccc
     3781 tgaaaaaagg aaaccggaac gggtttgccc gggcggaacg gctgattttc gatcaagtgt
     3841 ctcccccccc agcggtccgg tcttcggaag accggtttcg gtggctggga gacgtcctgg
     3901 atgcggaacg aaagggaatg ccggcggtct gggcgagaca aatcgctcgg gaactgggtc
     3961 ccgacgctcc ccccggctcc catttccttt aacgaggccc cgggacggcc caattgacgg
     4021 acgcatttcc gggagacgtg tgtccgaaga ccgcatcccc cctcctcgcc gtacaaagga
     4081 cgcaagggga gccgatggcg ggaaacatcc gggtccgatc cctcctcgaa aattctctca
     4141 agtccttaga ccatcccacc gattagtaca ccagagacca aagcgggcga tgggccgcgc
     4201 aaggtgattg gaactgtagg gtgggaaagt gcatcggttc aaccatgaca aggacgtcag
     4261 ggtaagcaac ttcaaggagg aacaaacatg ggacttcgaa tcaacaccaa cattgaggca
     4321 atggagatcc accgtcagtt ggttctgacc aacaacaagt tgggcgattc gctgaagagg
     4381 atctcatcgg gctatcgcat caattccgcc aaggacgacg cctcgggatt tgcggtggcc
     4441 aatatgttca aggccaagat ctcttccatg cgggtggcct accagaacgg cagtgaagcc
     4501 aacagcatgc tccaggtggc cgacggtgcg tacaacaaga tctatgatgt tttggtccga
     4561 atgaaggacc tggccaccca ggccgcttcc ggtcagacgg aaaaccggga caagctccag
     4621 gtggaatttg ccgaactgca ggcggaaatc gaccgcatcg cccagtcgac cacctacagc
     4681 accaagaacg cggccagtct gcagctgatc aacggcagca gcgacatttc cggcgggatt
     4741 acgttcatgg tgggggccac caacaacagc cagaacatga tcggcatcac tctcagcgcc
     4801 gcctgcaccg gggccctggg agtcggctcc aacttgctgt ccatcgattc cgtgggcagt
     4861 gctcgggccg ctatggatgc cctggatctc gccctgggtt ccatcaacac ctacatgggc
     4921 aaggtcggca gctaccagaa ccggctgcag atcaccatgg agaacctgag cacggggatc
     4981 gagaacttct ccgcctccga gtccaccatc cgcgacgtgg atatggccta cgaggtgatg
     5041 aatttcaccc gggaacagat cctgcaacag tcgggcatgg ccatgttggc ccaggcgaac
     5101 acggccccgc agcaggtcct gactctgctc cgataaacca tcgagtctat gacagggccc
     5161 cccggcacga aagggggccc ccgtgtacca tccatcctac agttcttgga aaggcggtct
     5221 tcgggccgcc ttttcatttt tcggtgaaaa gtcctttaag ggtataaaat cttctgaaaa
     5281 aagccttaag tgagctccga attccgccga tttaaatgcc agaagccaat gggaacgggt
     5341 tggcgtagga cggagtaagg accaaggagt ggataagtaa attcgctaat agtcatgaca
     5401 aggatgtcta gatacgtcgt ttcaaggagg aacaaaaatg ggacttcgaa tcaacacaaa
     5461 cattgaggca atcaccatcc atcgtcaact ggtcgagacc agcagcaaac tgggggattc
     5521 tcttaggaag atttcatcgg gctatcgcat caattccgcc aaggacgacg cctcgggatt
     5581 tgcggtggcc aatatgttca aggccaagat ctcttccatg cgggtggcct accagaacgg
     5641 cagtgaagcc aacagcatgc tccaggtggc cgacggtgcg tacaacaaga tctatgatgt
     5701 tttggtccga atgaaggacc tggccaccca ggccgcttcc ggtcagacgg aaaaccggga
     5761 caagctccag gtggaatttg ccgaactgca ggcggaaatc gaccgcatcg cccagtcgac
     5821 cacctacagc accaagaacg cggccagtct gcagctgatc aacggcagca gcgacatttc
     5881 cggcgggatt acgttcatgg tgggggccac caacaacagc cagaacatga tcggcatcac
     5941 tctcagcgcc gcctgcaccg gggccctggg agtcggctcc aacttgctgt ccatcgattc
     6001 cgtgggcagt gctcgggccg ctatggatgc cctggatctc gccctgggtt ccatcaacac
     6061 ctacatgggc aaggtcggca gctaccagaa ccggctgcag atcaccatgg agaacctgaa
     6121 cacgggcatc gagaacttct ccgcctccga gtccaccatc cgcgacgtgg atatggcctt
     6181 cgaggtgatg aacttcaccc gggaacagat cctgcagcag tccggcatgt ccatgctggc
     6241 ccaggcaaac acggccccgc agcaggtcct gaccctgctc cggtaatccc gggggtccaa
     6301 cccgggggct cccgctcacg aggcaggggg cccactgaac caaaccatcc cacatggttg
     6361 aaaaaggcgg ctgaccaccg cctttttctt ttggattttc cattccgtgc gatttttaag
     6421 aaccgagccc accggttatt ccctttgcct ttccatccgc cttccagcat tctcgaatca
     6481 ctgctgagtg taactcggtc tcatcttgtc tgcagttctc cgccacacag gaggatgcga
     6541 tgataagaaa agtcccggca gcagtgctca gcattccggc aacccttttc ttttccgcag
     6601 ccgtttccgt cttccggcgg ccgggagagg cgcctcacca gcagggctcc tcttcgagct
     6661 ccgggaacac caggaaaccg cccgtgcacg acaagtcctc accgtcgaag tcaagaagac
     6721 ctgccctatc cctccccgtg ttcacggcga atcaccgcat ccgccgcata agcagaaagc
     6781 tgtccgcatc cacaaaaagc ggtggtccac ctgccgtcca aatcgacgca caaggtccca
     6841 tcccgcccgg agcaggagcg gcggtcgaca attaataacg gaggagtacc atgggctggc
     6901 gcttcagagt cattcaagca tgggttttgt ggatttgtgt ccttttgcct ctgaaggtgg
     6961 acgccgtgac gaccacctac ggaggcgaga ccctcgaatg ggaggtccgg gcaggaggga
     7021 aacccctgga tccggcattc caggggtgtg aggacacttc ttggggtgtg gaatgcaact
     7081 atgggggagt gtttcccagc gtcggcgagc aactctccct cgccgccatc atctctccca
     7141 gccaatggtg ggacggctac aactgccttc agatggggac tatggacacc gaaagcataa
     7201 ccggctgcga gtgcaagccc ctgatgctct cgggaaccac gcttttctat ccccagtcgc
     7261 tcaagtactc cgtgcagatc gtctacatga gggcacaggt gtcccccatc ggccgcaact
     7321 atacgttcaa ggtgaacctg tagtaccttt tcggcagcga cccgaatgcc gtcggcgcac
     7381 gaccccatct caaaggaggc ccgattctta cgacagcctc ggacactgca gatccatgac
     7441 ggcccctgac gggcgggtgc ccgcctacgg ctacgatgtc gccggcaacc ttacgaccgc
     7501 ttcggatttc tacgggaaca ccacgacctt tcaatacgac gcgaacaact accttgtgtc
     7561 catgaccacc ggagataaga ccactcaatt tgcctacggt ggtgaggggt ccggcaagaa
     7621 gatttcttcg gtgacggacg cccttggctg gaccacccgg tacgaccagg cggacgcgac
     7681 aacggtccgt gtcgtcgatc cccgaggggg tgtctgggag tacgaaaacg aagacggaaa
     7741 gaccggcacc accaccgatc ctctgggccg gacgacgtcc agggtctaca agggcggaaa
     7801 cctcgcggct tacctggatg gcaacgccaa tctccggaac atgggtttcg atgcccgggg
     7861 aaacatggta tcctggcagg gtacggtctt gtggtcaacg ccgtcgtgac ccgggccacg
     7921 ggaacctgcg aaagaga
//
LOCUS       DAOOVC010000168         7905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5542_length_7905_cov_6.556561, whole genome shotgun sequence.
ACCESSION   DAOOVC010000168 DAOOVC010000000
VERSION     DAOOVC010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5542_length_7905_cov_6.556561"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="PLM79_15665"
     CDS             <1..1178
                     /locus_tag="PLM79_15665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOV87797.1"
                     /translation="NEGMPRYAAVVCKDITENKAAEQALRESQEILSGIVHSVADYIS
                     MTDDEMRVVWNNAAVNELFGPNLVGKKCHEAYYHRDGVCSECIVKKCLQDGKPHEEEK
                     QILGPDGRRMDVWSTATVAARHPDGRPKLVVKVYRNITEKNRLKAEALRAGHLASLGE
                     LAAGVAHEINNPINGIINYAQILADRNNGDQELARISNRIIKEGDRIANIVGNLLSFA
                     RDRKEEHQPVDVRKVLSDSLGLTEAYFFHKDGIKLVIDVPGELPRIKARSQEIQQVFI
                     NILSNARYALNQKYPGLHKDKILRISAQEIHTDDHRAVRVTFHDQGTGIASHLLDRIC
                     DPFFSTKPVGEGTGLGLSNSYGIVKEHGGNLTFESKFGKYTKVMVDIPCGDRVKDKA"
     gene            1175..2899
                     /locus_tag="PLM79_15670"
     CDS             1175..2899
                     /locus_tag="PLM79_15670"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOV87798.1"
                     /translation="MRAKILVIDDEESIRDTFSIFLTQEGHEVVTAADYAPAIEIISQ
                     DHLDLIFADIVLGGYTGIDVLREVKKRGLDCPVVLVTGKPSVETAAESVRLGAFDYLA
                     KPLRKDNLLRITNQALHHKALLDEKNRIEAEKEGYRLNLEAIFHSVQDGIITVDTEMK
                     VLECNQAAARICGLAPEEIAGKKFTEVMTHCRKSCCRMWGERIDLKTAVREFRVECGH
                     SRHARQLVVLSSSPLKDRNDRFRGAVLLIRDITRLTNLERELKERSKFHDIIGKSRRM
                     QDIYNLLEDLAETETTVLISGESGTGKELVAEALHLSSSRASHPLVKVNCSALSENLL
                     ESELFGHVRGAFTGAVKDKVGRFQLANGGTLFLDEIGDISPRIQLKLLRVLQEKEFER
                     VGDSNTMKVDVRIIAATNRSLREKVRLGEFREDLYYRLKVVEITLPPLRERLEDVPLL
                     IKQFRKHFNNSLKKNIEGISDEAMSLLMNYPWPGNVRELKHAMEHAFILCRGQTILVE
                     HLPADIRESPGTYARKAPVEKQSGITAEQILKALNQTDWNKAKAARVLGISRQTIYRK
                     IEEHRITR"
     gene            complement(3112..4503)
                     /locus_tag="PLM79_15675"
     CDS             complement(3112..4503)
                     /locus_tag="PLM79_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV87799.1"
                     /translation="MEKTPSPKPTILVVDDDVHILEVLDARLSSAGFHVLRAADAHRA
                     MDLIRAHPVRLVISDVRMPGKGGMDLLAEALSLRPDLPVIFLTAYGTIPDAVQAVKAG
                     ATEYLTKPFDGRELLQKVQEVLKSRQPKVAEEGLPPIQQVLSGGNSPAMKELYDLIDR
                     IAPSDVNVLILGESGVGKERVARLIHQRGPRREHPFVVVDCGSTPTGLLESELFGHVR
                     GAFTHAIRDKKGLIEASDRGTLFLDEIGNISPEMQVRLLRFLEDRKIRKIGDLKEIPV
                     NCRVISATNLDLPEEVENGDFREDLYYRLRVVTLRIPPLRDRREDIPLLAAHFVKGFC
                     ESHGLPLATLPPETMKWLCEYPWPGNVRELKNALEAGVVLCKNGVLRASDLHLSGLPH
                     ARHKPPAAPADSLSLEESERHAILRALQQAGWVQKDAAQLLGISRRAIHYKIKKYGIE
                     VAGKKSSEDLPGD"
     gene            complement(4516..5181)
                     /locus_tag="PLM79_15680"
     CDS             complement(4516..5181)
                     /locus_tag="PLM79_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87800.1"
                     /translation="MPWTRILAAAFLGFLGGCGSMPGAHGPVDKPPVISAPVISPSDA
                     ENKTWENGVQAFSREDYQGAQAYFEALTESAENPALSHKALYALACTRLVLAQTSEEF
                     SEALTLWECWSRKASAQGCEEDPRMLTPFLERVTQPILMDEQSEQAARIRKKMESKNI
                     LLYRSLQEYKNLLRDREKEVERMSNRLEAKEKEIRRLRHQIDSLEAIHLKIQEKKKEA
                     SAP"
     gene            complement(5218..6675)
                     /locus_tag="PLM79_15685"
     CDS             complement(5218..6675)
                     /locus_tag="PLM79_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOV87801.1"
                     /translation="MKIRFGIMIKLFVWYFAMVFIFYATILVLFVDIRQIVSISENLV
                     NKNYKISSASKKMIESLLWMEESQNKYDLLKKQDYKQYFISAQREFERNLEEILVLRT
                     REGTAEDPWDRLYQHYRAQLPQSGEGLDEEEPSKVLWIPEAILNDWVQRISKARASNE
                     LAIESEMRTLTDRGRMAVRWGFLGLGFSVLLGLMGGIFLTHSMVRPLRELRKGIRDIS
                     HEGLREPIGITSKDEFGELAGAFNEMAVRLKEEERMRSDFVSMLSHEIRTPLTSIRES
                     VNLIVEEVMGEINDRQRRFLQIASSEIERISDLLNHLLQVSRLEAGGLRIHPVPISPS
                     HFVTGSIDRLAPAAEAKGILVSPQMAPNLPRVMADAEHLQQVMLNLIGNAIKFSPAGT
                     EILVRVDRDFAEGSDRVRFSVSDAGPGIPEKELSMVFHKYYRASGVRKQMDGVGLGLS
                     ISKHIVEAHGGSIWVESQVGRGSTFGFTLPAVSEE"
     gene            7247..>7905
                     /locus_tag="PLM79_15690"
     CDS             7247..>7905
                     /locus_tag="PLM79_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87802.1"
                     /translation="MQKPASVRAAFGVLLLGAFLFTASISSSQTGQNGPWKRIDEGLH
                     LGTFDAPQKSEVGDSKITVLKADPTLYTLKLLCASEHSWKKLTVKEWCRQHQLLAGVN
                     AGMYQEDGYTSVGYMKNFLHINNPGLNGNNTILAFNPTDPSLPQTQIIDRECQDFHSL
                     KNKYQTMIQSIRMISCNRKNVWHPQQGKWSILAIAIDSGGNLLILFSQSPYSVYDFIN
                     NL"
BASE COUNT         1769 a         2346 c         2256 g         1534 t
ORIGIN      
        1 acaacgaggg aatgcctcga tacgccgccg tggtgtgcaa ggacatcacc gagaacaagg
       61 ccgccgaaca ggcacttcga gaaagccagg agatcctctc gggcatcgtc cattccgtgg
      121 ccgactacat cagcatgacc gacgacgaga tgagggtcgt ctggaacaat gcggccgtaa
      181 acgagctgtt cgggccgaac cttgtgggaa aaaagtgcca tgaagcctac taccatcggg
      241 acggcgtctg cagcgagtgc atcgtcaaga agtgcctcca ggacgggaag ccccacgagg
      301 aagaaaagca gattctgggc ccggacggaa gacggatgga cgtttggtct acggccacgg
      361 tggctgcccg gcatccggac ggacgcccca agctggtcgt caaggtctac cgaaacatca
      421 ccgagaagaa tcggctgaag gcggaggccc tacgggccgg gcacctggca tccctgggtg
      481 aactggccgc cggcgtggct catgaaatca acaatcccat caacggaatc atcaattatg
      541 cccagatcct cgcggaccgg aacaacggtg atcaagagct ggcaaggatc tccaacagga
      601 tcatcaagga aggcgaccga attgccaaca tcgtcgggaa ccttctctcc tttgcccggg
      661 atcgcaaaga ggaacaccag ccggtggatg ttcgaaaggt cctgtccgac tccctgggcc
      721 tcaccgaggc ctactttttc cacaaagacg gcatcaaact ggtcatcgac gtgcccggcg
      781 aactcccccg aatcaaggct cgaagccagg aaattcagca ggttttcatc aacatcctga
      841 gcaacgcccg atacgccttg aaccagaaat accccggact tcataaggac aaaattctgc
      901 gtatatccgc gcaggaaatc catacggacg accatcgagc cgtgagggtc acctttcacg
      961 accaggggac cggaattgcc tcccacctcc tggaccgcat ctgcgatcct ttcttttcca
     1021 ccaagccggt gggggaggga acgggcctcg gattgagcaa cagctacggc atcgtcaaag
     1081 aacatggagg aaatctgacc ttcgagagca agttcggaaa atacaccaag gtcatggtgg
     1141 acattccgtg cggcgaccgg gtgaaggaca aggcatgaga gcaaagatcc tggtgatcga
     1201 cgacgaagaa agcatccgag acacgttcag catcttcctg acccaggagg gccacgaagt
     1261 cgtaacggcg gcggactacg cgcccgccat cgagatcatc tcccaggacc acctcgatct
     1321 gatcttcgcg gacatcgtgc tgggaggata cacggggatt gatgtgcttc gagaggtaaa
     1381 gaagagaggc ctggactgcc ccgtggtgct ggtaaccgga aagcccagcg tggaaaccgc
     1441 ggcggaatcg gttcgactgg gggccttcga ctacctggcc aagcctcttc gtaaggacaa
     1501 cctgcttcgc atcaccaacc aggcccttca tcacaaggcc ctcctggacg aaaagaaccg
     1561 gatcgaagcg gaaaaggaag gatatcgtct caacctcgag gccatctttc acagtgtcca
     1621 ggacggcatc attaccgtgg acacggaaat gaaggtgttg gagtgcaatc aggcggcagc
     1681 ccggatttgc ggcttggccc ccgaggaaat cgcgggcaag aagttcaccg aggtcatgac
     1741 ccattgccgc aagtcctgct gcaggatgtg gggggagagg atcgatctga aaaccgccgt
     1801 ccgggaattc cgcgtcgagt gcgggcactc ccggcacgcc cgacaactgg tggtcctcag
     1861 cagttccccg ctaaaggacc ggaacgaccg gttcaggggg gcggtgctgc tcattcgaga
     1921 catcacccga ctgacaaacc tcgagcgaga actcaaggaa cgttcgaaat tccacgacat
     1981 catcggaaaa agccgacgca tgcaggacat ctacaacctc ctggaggatc tcgcggaaac
     2041 ggagaccacg gtcctcatct ccggggagag cggtacgggg aaggaactgg tggccgaagc
     2101 cctgcacctg agcagcagcc gtgcgagtca tcccctggtg aaggtgaact gttcggccct
     2161 ttcggagaat ttactggaga gcgagctttt cggccatgtg cggggggcct ttacgggcgc
     2221 cgtcaaagac aaagtgggac gatttcaact ggccaacgga gggacccttt tcctggacga
     2281 aatcggcgat atctcccccc ggattcagct gaagcttctg cgcgtgctcc aggaaaagga
     2341 attcgagcgg gtcggggatt ccaacaccat gaaggtggat gttcgaatca ttgcggccac
     2401 caatcggagc ttgagagaga aggtgcggct gggggaattt cgcgaagacc tgtactaccg
     2461 cctcaaggtc gtggaaatca ccctgccccc tcttcgagag cgcctcgaag acgtgccgct
     2521 gctcatcaaa cagtttcgca aacacttcaa caactccctc aagaaaaaca ttgaagggat
     2581 ttccgacgaa gccatgagcc tactcatgaa ttacccttgg cccggcaatg tgcgggaatt
     2641 gaaacacgcc atggaacatg ccttcatttt atgccgaggc cagaccattc tggtggagca
     2701 cctccctgcg gacattcgag aaagcccggg cacgtacgct cgaaaggccc ccgtggaaaa
     2761 gcaatcggga atcactgccg agcagatcct caaggctttg aaccaaaccg actggaacaa
     2821 agccaaagcg gcccgagtcc tgggcatcag ccgccagacc atctaccgga aaatcgaaga
     2881 acatcggatc actcggtaga gcgtcgcaga ggggaccttc cgactccgaa ccgagaaaag
     2941 agggaacccg ccggcgctcc gagacaacac aaccctctcg agaacccgat tgtcgtcacc
     3001 cgtaatccgg tacatcgatg tcacgtaaca tgtgtgtatt gttacaggat tcttccccgg
     3061 gagagcggtg acggggggaa aggtttcggg tactgggaca aggtcggagt atcagtcgcc
     3121 gggaaggtct tcggagctct ttttcccggc cacttcgatt ccgtatttct tgatcttgta
     3181 gtgaatggcc cgtcgactga tacccaggag ttgagccgca tccttttgca cccacccggc
     3241 ctgctggagg gcacgaagga tggcatgccg ctcgctttcc tccagggaca acgagtcggc
     3301 cggagctgcc ggcggtttgt ggcgggcgtg gggaagcccg ctcaggtgca ggtcgctggc
     3361 gcgcagcact ccgtttttgc agagcaccac ccctgcctcg agggcgtttt tgagctcccg
     3421 gacatttccc ggccagggat attcgcaaag ccatttcatg gtctcgggtg gaagggtcgc
     3481 aaggggcaac ccgtggctct cacagaaccc tttgacaaaa tgggccgcca gcagggggat
     3541 gtcctcccgc cggtccctca ggggagggat ccgcagggtc accacccgga ggcgatagta
     3601 gaggtcttct cgaaagtctc cgttttccac ttcctcggga agatccaggt tggtggccga
     3661 tatcactcga cagtttacgg ggatctcctt caggtcccca attttgcgga tcttgcggtc
     3721 ttccaagaac ctcaggagac gcacctgcat ttcgggggag atgttgccga tctcgtccag
     3781 aaaaagggtc ccccggtccg aagcctcaat gaggcccttt ttgtctcgga tggcatgagt
     3841 gaacgctccc cgaacgtgac caaacaactc gctctccaaa aggccggtgg gtgtggagcc
     3901 gcaatccacc accacgaagg ggtgctcccg tcgcggccct ctctggtgga tcagccgggc
     3961 caccctttcc ttccccactc cgctctctcc caggatcagc acattcacgt cgctgggagc
     4021 aatccggtcg atcaaatcgt agagctcttt catggcgggg ctgtttcctc ccgataggac
     4081 ctgctggatg ggaggaagcc cttcctcggc gactttgggc tgccggcttt ttaggacttc
     4141 ctggactttt tggaggagtt cccgaccgtc gaagggtttg gtgaggtatt ccgtcgcccc
     4201 ggctttcacg gcctggacgg cgtcggggat ggttccgtag gcggtgagaa agatcaccgg
     4261 caaatcgggg cgcagggaga gggcctcggc caggagatcc atgccccctt tgcccggcat
     4321 ccggacatcg gatatgacca gtctcaccgg gtgtgcccgg atcaggtcca tggcccgatg
     4381 agcatccgca gccctgagga catggaaccc cgccgaggag agtcgcgcat ccaggacctc
     4441 gaggatgtgc acatcatcgt ccaccaccag gatggttggc ttaggactgg gggtcttttc
     4501 cacaaaccac ctttctcacg gggcagaagc ctctttcttt ttctcctgga tcttcaggtg
     4561 aatcgcctcc agggagtcga tctggtgccg gagccgtcga atctcctttt ccttggcttc
     4621 gagcctgttg ctcatccgct ccacctcttt ttcccgatct cgaaggaggt ttttatactc
     4681 ctgtagggag cgataaagaa ggatattctt ggattccatc ttctttcgaa ttcgagccgc
     4741 ctgctcggac tgttcatcca tgaggatcgg ctgggttact cgttccagaa agggggtgag
     4801 cattcgggga tcttcctcgc aaccctgggc ggaagctttc cgactccagc attcccaaag
     4861 ggtgagggct tcgctgaact cctccgaagt ctgagccaga accaatcggg tgcaggcaag
     4921 ggcgtacagg gctttgtggg agagagcggg gttttccgcg ctctcggtca aggcctcgaa
     4981 ataggcctgg gccccctggt aatcctcccg ggaaaatgcc tgaaccccgt tttcccatgt
     5041 tttattctcg gcgtcactgg gggaaatcac gggagcggaa atcaccgggg gtttgtccac
     5101 ggggccgtga gcccccggca ttgaaccgca tcctcccaga aatcccagaa aagccgccgc
     5161 caaaatcctg gtccagggca tagtattcct ccccacgatc ttgcacaaac ccatgaacta
     5221 ctcctcggac accgccggaa gcgtgaagcc gaaggtgctc ccgcgcccca cttgactttc
     5281 cacccagatg ctccccccgt gggcttccac gatgtgcttg gagatactga gccccaggcc
     5341 gactccgtcc atctgcttgc gtaccccgga agcccgataa tacttgtgaa acaccatgga
     5401 cagttccttt tcggggatcc ccggacctgc gtcggacacg gaaaaccgca cacgatccga
     5461 tccttcggca aaatcccggt ctacccggac caggatttcg gttcccgccg gagaaaactt
     5521 gatggcgttt ccgatgagat tcagcattac ctgctggaga tgctccgcgt ccgccatgac
     5581 gcggggaaga ttcggcgcca tttgggggga aacgagaatc cccttggcct cggccgccgg
     5641 ggccaatcga tcgatgctgc ccgtcacgaa gtgggacggg gagatgggga ccggatggat
     5701 gcgcagcccc ccggcctcga gccgggatac ctggagaaga tggttcaaca gatcgctgat
     5761 ccgctcgatt tcactgctgg caatctgcag gaatcgcctc tgccggtcgt tgatttctcc
     5821 catgacctct tcgacaatga gattgaccga ttcgcgaatg gaggtgaggg gtgtacggat
     5881 ttcatggctc agcatggaga cgaagtccga gcgcatgcgt tcctcctcct tgagtcgaac
     5941 cgccatctcg ttgaaggccc ccgccagttc cccgaactcg tccttggaag tgattccgat
     6001 gggctcccgc agtccttcgt gggagatgtc gcgaatgcct ttgcgaagct cccgcagggg
     6061 acggaccatg gagtgggtga ggaagatgcc ccccatgagc cccagcagga cggaaaatcc
     6121 caaccccaaa aaaccccacc gcaccgccat gcggccgcgg tccgtcaggg tcctcatctc
     6181 cgattcgatg gccagctcat tgcttgcccg tgccttggag atcctctgga cccagtcgtt
     6241 gagaatggcc tcgggaatcc acaggacctt ggagggttct tcctcgtcca ggccttcgcc
     6301 cgattggggc aactgggcac gatagtgctg gtacagccga tcccaggggt cctcggcagt
     6361 gccttcccgg gtcctcagaa ccaggatctc ctcgaggttc ctctcgaact cgcgctgggc
     6421 cgagatgaag tactgcttgt agtcctgctt cttgagcaga tcgtacttgt tctggctttc
     6481 ctccatccaa agtaggcttt cgatcatctt cttggaggcg gaggaaatct tgtagttctt
     6541 gttgaccagg ttctcggaaa tgctgacgat ctggcgaatg tcgacgaaga gcaccaggat
     6601 ggtcgcgtag aagatgaaga ccatggcaaa ataccagacg aaaagcttga tcatgatgcc
     6661 gaatctgatc ttcatccgca ttccgtgatt cttcctcgcg tctccgggac cgattcatgc
     6721 cgttgcccag ggcctcagcc gcaaacgagc ggcttaccct cccatcggcc ctgcccgcac
     6781 gggagggaag acccccccac ccctggatgg gaaaggatcg aggagaaggg gaaccgcctg
     6841 cctgagaatc attggataaa gtcacccggg ggaagggagg gcgaaacctt cgtgtgtccg
     6901 cggtcccgca ggtcggccgt cttcggttga ccggaaaatc atagtggaga aagacaacgg
     6961 agtcaattcc gcctctccca cccgggggga aacccttggg aaactgagga agacgccggg
     7021 cgagaagacc cgctgcaaaa agcacagagc ccgcggacgg ggggaacgca tcggaccttc
     7081 cacggcatta cggcttcaca cggagaacga ttggatgtaa gatggtcccg cggatccggg
     7141 gtctttccag agagggaaaa ccatgggatg gatgtggaaa agaacctttc atgacccggc
     7201 ttcacaggag cttccatttc tccgcaaccg catgaggttt ctccgcatgc aaaaacccgc
     7261 ttcggttcgc gccgctttcg gtgtcctcct cctgggcgct ttcctgttca ccgcctcaat
     7321 ctcctcgtcc caaaccggac aaaacggtcc ctggaagcga atcgacgagg gacttcacct
     7381 gggaaccttc gacgctcccc agaaatcgga ggtcggcgac tcgaagatca cggtcctgaa
     7441 agcagacccg actctctaca ctctcaagct cctatgcgcc tcggaacaca gctggaaaaa
     7501 gctcacggtc aaggaatggt gtcggcaaca ccaactgctg gcgggagtga acgccgggat
     7561 gtaccaggag gacggatata ccagcgtcgg ctatatgaag aactttctgc acatcaacaa
     7621 cccgggcctc aacggcaaca acaccatcct tgccttcaat cccaccgacc catccctgcc
     7681 ccaaacccaa atcatcgacc gcgaatgcca ggatttccat tctctcaaga acaagtacca
     7741 aaccatgatc caaagcattc ggatgatctc ctgcaatcgg aaaaacgtat ggcatcctca
     7801 gcaggggaaa tggagcatcc tggccatcgc catcgattcc ggcggcaatc tcctgatcct
     7861 tttttcccag tcaccgtatt ccgtatacga tttcatcaac aacct
//
LOCUS       DAOOVC010000169         7879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5577_length_7879_cov_5.944402, whole genome shotgun sequence.
ACCESSION   DAOOVC010000169 DAOOVC010000000
VERSION     DAOOVC010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5577_length_7879_cov_5.944402"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1324)
                     /locus_tag="PLM79_15695"
     CDS             complement(377..1324)
                     /locus_tag="PLM79_15695"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOV87803.1"
                     /translation="MGGRHNEDTVRRYEGNPILTRADVPYAVETVHNAAVAKHEGSYV
                     MLFRSHLRNGRSVIGLARSDDGFRFQVAPEPFLTPAREGLFAEYEEFGVEDPRICPME
                     GSYLITYSAYSRHGVRIGLARTRDFKVLERVALITQADCRNVVLFPERFRGLFARLDR
                     PHSEISPWSIWLSYSPDLVHWGDSRVVIKPLSYHWDEMKIGPGAPPIKTEKGWLNIYH
                     GVFKTMDGAVYRLGVALHDLEDPARVLGVSDEWILQPEDPWEVTGYVHNVVFTCGAIA
                     EDDGTLKLYWGGADTVMCAGTARIDALIDLCLARPRTPL"
     gene            complement(1308..2651)
                     /locus_tag="PLM79_15700"
     CDS             complement(1308..2651)
                     /locus_tag="PLM79_15700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87804.1"
                     /translation="MLSPIAWRTPPRHYGPWESVVSLLTEGLVRRGIEVTLYATADSV
                     TSGKLKAVCPSGYEEDRELDPKVWECLHISELFECADQFDLIHNHFDFLPLTYLRMTA
                     APVLTTIHGFSSQKVLPVYKKYSGRAYYVAISNADRHPELDYIATIHHGIDLEAFPFK
                     PEQGEYLLFFSRIHPDKGAREAIEIARRARKRLVMAGIIQDRDYFEAEVKPHLDADRI
                     EYVGSVGPERRKDLLGGAFALIHPVNFEEPFGLSVVEAMACGTPVVAFSRGSMPELVV
                     DGVTGFLVASIDEAVKALGRIPSIGRIACRRWVEEKFSAERMIEDYVKVYERILSVRK
                     REDLRPWGFYRTLCDEPNQKVKKIVVNPGQRLSLQRHRYRDEHWFMVKGHARVTRNSE
                     EIHLKAGQSMDIPRGTWHRVMNPGSEHVVFIEVQTGDYFGEDDIERIEDDYGRAA"
     gene            complement(2692..3633)
                     /locus_tag="PLM79_15705"
     CDS             complement(2692..3633)
                     /locus_tag="PLM79_15705"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87805.1"
                     /translation="MPERDLFKRYPGNPIITSENLSYRANSVFNAGAAAMEGQTLLLM
                     RVEDRRGMSHFTAARSRDGWSGWSIDDKPTLLPSPLDHPEEIWGIEDPRITYLEELGC
                     WAVTYTAFSRGGPLVSLACTKDFVKFERYGPVLPPEDKDAALFPVRFKGRWAMIHRPV
                     SNWPGAGADIWISFSPDLRHWGDHRVLIAARKGGWWDANKIGLSPPPLPTEEGWLILY
                     HGVRTTASGSIYRLGLALLDLEDPCNVIARADEWVFSPEESYELMGDVDNVVFPCGWV
                     REGNTVRLYYGGADKCLALAVAEVPELLAWLKEHNVN"
     gene            complement(3670..4896)
                     /locus_tag="PLM79_15710"
     CDS             complement(3670..4896)
                     /locus_tag="PLM79_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87806.1"
                     /translation="MSSGPIIIISSYPPRLCGIATFAEEAREFIEKANPGREVLVISH
                     LDGEGEGVFPIIDMGRHDWWRPVAEKVRELSPYVVHIEHEYGLYEYRDNRGIGDRNEG
                     FLTLLEAIGEYPIVVEPHTVHGRLRDFEANFLYELCRRSHVVLFKCHYQKWRLDWTFP
                     GYGWETPRNVMVVPHGARPDRRWGAHEIPGLREELGLDKIGLAEHVVGMIGWIQTNKR
                     WDILLSMWEEIREEIFRRTGQIWDLLAAVAMRDPGDRSIYEKWKADALELERRGFAHY
                     YEFIPRGEIYYKMMAVCDFIVLPSTDETQSGTLARVIALNKPYITTAPMEGLTAQTLE
                     SEGGLLFTTKKMLRDKVVKLACDENLRFTLGENLRRYLDEVVSWDLVAKRYNEAYELA
                     LEARRTGAPVTLEPEF"
     gene            complement(4814..7282)
                     /locus_tag="PLM79_15715"
     CDS             complement(4814..7282)
                     /locus_tag="PLM79_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV87807.1"
                     /translation="MDRAGLSPPNLHNVALIGNYLPRQCGIATFTSDLLEALSSEAPE
                     TSCWAMVMNDTPEGYPYPDRVRFELNQKNLADYQLAADFLNMSQVDVVCLQHEFGIFG
                     GSFGGHILELLRSLRMPVVTTLHTVLKEPNPRQKVVIRELGALSDRLVVMSRKAEAML
                     LDVYGIAPEKIAVIPHGIPDVPFVDPNFYKDQFGVEGRRVLLTFGLLSPGKGIEHMID
                     ALPEIVSRYPDATYVVLGATHPHVKNDQGESYRLSLQRRARELGVGDHLIFHNRFVDL
                     KELCEFLGAADIYVTPYLNREQIVSGTLAYALGAGKATVSTAYWYAQEMLEEGRGRLV
                     PFRDSKALAREILDLFENETERHAMRKRAYTFCRGMVWKEVARKYLEVFSEVRKEREK
                     HPRPVFHAKTLDAAPPELPQPCFDHLLLLTDDTGILQHAKFTVPDRIYGYCTDDNARA
                     LIAVLLAREMIPEEKDLTRLACRYLGFVHHAYNEENGRFRNFMTYERRWLEDEGSEDS
                     HGRAVWALGVAVALAASESLSAVALNLFNRALPVMTKFHSPRALAFGLVGIHNYLSRF
                     GGDSEVRRVRSVLAGRLYGLYKSNAADGWPWIENTVNYANARIPQAMVLSGWGMQDRE
                     MLEAGLQSLEWLIRIQTDPGGHFVPIGNRGWLTREGHRARFDQQPIEAQCMIEACVDA
                     YRITGERMWTLEAGRCFEWFLGANDLSVSLYDYGTGGCRDGLAADGANQNQGAESTLA
                     WLISLLTMHRLSGLEAVSRSDPAFKGAVDSVHHELVELEADRIQRGHTSISTGDRDVQ
                     RSDYHHQFVSSKALRHSDVCGRSS"
BASE COUNT         1593 a         2277 c         2313 g         1696 t
ORIGIN      
        1 tcaaatggat ttttattcat gagtttcttt taccgaaact tttgcagaat gcatgtaagt
       61 gactgaaaag gtttgaaatg agtgggaaga atggctatac gccttaagga tttccggcaa
      121 gaaacttaag gaggcatagc catggttttc ttcccgatct ggattgtacc gttttggggt
      181 tcgtttcgga actgttttgg tgcaagtggg tttcgatact ccctcggttt ccggtgggtc
      241 ggatgagaac cctcgggttt tcctcccgat ggacgatgag gtctcctctc gagcacgacc
      301 atgctctctc ttgctgcaat cctcttcgct tcttcttcac tgccgcgggg tctttggcct
      361 tggttgcgcc ccgttttcaa agaggtgttc ttggccttgc aagacaaagg tcgatgaggg
      421 cgtcgatcct tgccgtgccg gcacacatga ccgtatcggc ccctccccag tagagcttca
      481 gcgtgccgtc atcctccgct atcgctccgc aggtgaagac cacattgtgc acgtagccgg
      541 tcacttccca ggggtcctcg ggctggagaa tccattcatc ggatacgccg aggacccgtg
      601 cggggtcttc gaggtcgtgc agggcgacac ctagccggta tacggcaccg tccatcgtct
      661 tgaaaacccc gtggtagatg ttgagccagc ctttttcggt cttgatgggt ggggctccgg
      721 gtccgatttt catctcgtcc cagtggtagg aaaggggctt tatgaccacc cgggaatccc
      781 cccagtgaac gaggtccggg gagtaggaaa gccatatgga ccagggagaa atctccgaat
      841 gggggcggtc gagcctggcg aagagcccgc ggaacctttc cgggaacagg acaacgtttc
      901 ggcagtcggc ctgggtgatc agggcgactc tttcaaggac tttgaaatca cgggtgcggg
      961 caagtccaat gcggactccg tggcgggaat aggcgctgta ggttatgaga tacgatccct
     1021 ccatgggaca gatgcgcggg tcctcgaccc cgaattcctc gtattcggca aagagcccct
     1081 cccttgcggg ggtgaggaag ggttcgggtg cgacctgaaa gcggaagccg tcatcgctgc
     1141 gggccagtcc gatcacggat cgcccgtttc gaagatgcga gcgaaagagc atgacgtagc
     1201 ttccttcgtg ttttgccacg gccgcgttgt gcacggtttc aaccgcatag ggcacatcgg
     1261 cccgggtgag gatcggattg ccttcgtagc gcctgactgt gtcttcatta tgccgcccgc
     1321 ccatagtcgt cctcgatgcg ctcgatgtca tcttctccaa aataatcccc ggtttgcacc
     1381 tcgataaaaa ccacatgctc actgcccggg ttcatgaccc ggtgccaagt gccgcgcggg
     1441 atatccatcg actggccggc tttaagatgg atctcttcgc tgtttcgcgt aacccgagca
     1501 tggcccttaa ccatgaacca atgctcatcc cggtacctgt ggcgctgaag actaagcctc
     1561 tgcccggggt tgaccacgat cttttttacc ttctggttcg gctcgtcgca gagggtcctg
     1621 taaaagcccc aggggcgaag gtcctcgcgt ttccgcacgg aaaggattct ttcatagacc
     1681 ttgacgtagt cctcgatcat tctttcggcg ctgaactttt cttcgaccca ccttctgcag
     1741 gcgatgcgcc caatggatgg aattcttcca agggccttca ccgcttcatc gatagaagcg
     1801 accaaaaagc ccgtaactcc gtccacgacc aattccggca tgcttccccg gctgaaggca
     1861 accacgggtg tgccgcaggc catcgcctcc actacggaga gcccgaaggg ctcctcgaaa
     1921 tttaccggat gaatcagggc gaaggctccg ccgagaagat ccttcctcct ctcggggccg
     1981 acactgccca cgtactcgat acggtccgcg tcaaggtggg gttttacctc ggcttcaaag
     2041 tagtccctgt cctggatgat gccggccatg acgagccgct ttcgggccct gcgggctatc
     2101 tctatggcct cccttgcgcc cttgtccgga tggatgcggc tgaagaaaag aagatattcg
     2161 ccctgctcgg gcttgaaagg gaaggcctcg agatcgattc catggtggat cgttgcgatg
     2221 tagtcgagct ccgggtgccg gtctgcgttg ctgatggcaa cgtagtaggc acgtccgctg
     2281 tattttttgt agacgggaag gaccttctgc gaggaaaagc cgtggatggt ggtgaggacc
     2341 ggagccgcgg tcatcctcag gtaggtgaga ggcaggaaat cgaaatggtt gtgaatgagg
     2401 tcgaactggt ccgcgcactc gaaaagctcg gatatatgaa ggcattccca caccttggga
     2461 tcgagctccc tgtcttcctc gtatcccgag gggcaaaccg ccttgagctt gccggaagta
     2521 acggagtcgg cggtcgcata caatgtaacc tctatgcccc tgcgtaccag gccttcggtg
     2581 agcagcgaaa caacgctctc ccagggcccg tagtgccggg gcggagtacg ccaggcaatc
     2641 ggtgaaagca tcgctactcg cattggcaac taccttttca attcagtccc tctaattcac
     2701 attatgctcc ttgagccatg ccaaaagttc gggaacctcc gccacggcaa gggccaggca
     2761 tttgtcggcc ccgccgtaat agagccgcac ggtgttcccc tctctgaccc agccgcaggg
     2821 aaacacgacg ttatccacat cacccatgag ttcatagctc tcttcgggag agaagaccca
     2881 ctcatcggcg cgggcgatca cattgcacgg gtcttcgaga tccaaaagag ccaggcccag
     2941 ccggtagatc gagccgctcg ccgtcgtgcg cactccgtgg tagagaatga gccatccttc
     3001 ctctgtggga agcggcgggg gggagaggcc gatcttgttt gcgtcccacc agccgccctt
     3061 tcttgccgcg atgaggacgc ggtgatcgcc ccagtgcctg agatccggtg aaaaggatat
     3121 ccagatatcg gcccccgctc cgggccagtt ggaaaccggc ctgtggatca tggcccagcg
     3181 ccccttgaaa cggacgggaa agagagctgc atccttgtct tcgggcggaa gcaccggtcc
     3241 gtagcgctcg aacttcacaa aatcttttgt gcaggcaagg gagacgaggg ggcctccccg
     3301 ggagaaggcc gtgtaggtca cggcccagca gcccagttct tcgagataag tgatcctcgg
     3361 gtcctctatt ccccatattt cctcgggatg atccagcgga gagggaagaa gggtgggttt
     3421 atcgtctatg ctccagccgc tccatccgtc gcggcttctt gccgccgtaa aatgggacat
     3481 tccccttctg tcctcgacgc gcatgagaag gagggtttga ccttccattg cggcggctcc
     3541 ggcattgaaa acggagttcg cccggtagga aaggttttcg gacgtgatga tgggatttcc
     3601 gggatagcgt ttgaagaggt ctcgttccgg catgcttcga tttcgctcct ccacaggaaa
     3661 accaacgact cagaattcag gctccaacgt cacgggagcc cccgtgcgcc ttgcctccaa
     3721 ggcaagttca taggcctcgt tgtatcgctt cgccaccagg tcccaggata cgacctcatc
     3781 gagatagcgc ctcaggtttt cacccagtgt gaagcgcagg ttctcgtcgc aggcaagctt
     3841 tacgaccttg tcccggagca tcttttttgt ggtgaaaaga aggcccccct cgctctccag
     3901 ggtctgtgcg gtcagcccct ccatgggagc ggtggtgatg tagggcttgt tcagggcgat
     3961 aacacgggcg agggtgccgg actgggtttc atccgtcgag ggcagtacga taaagtcgca
     4021 gaccgccatc atcttgtagt agatctcacc cctcgggatg aactcatagt agtgggcgaa
     4081 gcctcttcgc tcgagttcga gggcatcggc tttccacttc tcgtagatcg atcggtctcc
     4141 cggatcgcgc atggctacag cggcgagaag gtcccagatc tgaccggttc ggcggaatat
     4201 ctcctccctt atctcttccc acatggaaag caggatgtcc cagcgcttgt tggtttgaat
     4261 ccagccgatc atgcccacaa cgtgttctgc aaggccgatc ttatcgagtc caagctcttc
     4321 tctcaggccg gggatttcgt gggctcccca gcgcctgtcg ggtcttgccc cgtggggcac
     4381 gaccatgacg tttctcgggg tctcccagcc atagcccgga aaagtccagt cgagccgcca
     4441 cttctgatag tggcacttga agaggaccac atggcttctc cggcagagtt cgtagaggaa
     4501 gttcgcctcg aagtcccgaa ggcgcccgtg cacggtgtgg ggctcgacca cgatgggata
     4561 ttcgcctatg gcctcgagga gggtgagaaa cccctcgttt cggtcaccga tgccccggtt
     4621 gtccctgtac tcatagaggc cgtattcgtg ctcgatatgc accacataag gcgaaagctc
     4681 ccttaccttc tcggccacgg ggcgccacca gtcgtgccgg cccatatcga tgatggggaa
     4741 aaccccctcg ccctctccgt caagatggct tatgaccagg acttcccgcc ccggattcgc
     4801 cttttctatg aattcacgag cttcttccgc aaacgtcgct atgccgcaaa gccttggagg
     4861 atacgaactg atgatgataa tcggaccgct ggacatccct atctccggtt gaaattgacg
     4921 tgtgtccccg ttgaatccgg tcggcttcga gctcgacgag ctcatggtga accgaatcga
     4981 cagccccctt gaaagccgga tcgctcctgg ataccgcctc gaggccgctc agccgatgca
     5041 tggtaagaag cgagatgagc caggccaggg tcgattccgc tccctggttc tggttggcgc
     5101 cgtccgccgc aaggccgtcc cgacaacccc ccgtgccgta gtcgtacaga gagacactga
     5161 ggtcgtttgc cccgagaaac cactcgaagc agcgcccggc ttccagcgtc cacatcctct
     5221 cgccggttat tctgtaggca tccacacagg cttcgatcat gcactgggct tcaatgggct
     5281 gctggtcgaa tcttgccctg tgaccctccc gggtaagcca gccgcggttg ccgatgggta
     5341 cgaaatgccc accggggtcg gtttgaatcc ggataagcca ctcaaggctt tgaagccccg
     5401 cctccagcat ctcgcggtcc tgcatgcccc atcccgagag caccatggcc tggggaatcc
     5461 ttgcgttggc ataattcacc gtgttctcga tccaaggcca gccgtcggcg gcgttggact
     5521 tgtagagccc atagagcctt ccggccagga ccgagcggac ccttcgcacc tcgctgtctc
     5581 cgccgaagcg gctgagatag ttgtgaatgc ctaccagtcc gaaagccagg gccctggggg
     5641 aatggaactt cgtcatgacg ggaagcgccc ggttgaagag gttcaatgcg acggcggaaa
     5701 gactctcgga ggctgcaagc gccaccgcca cgcccagggc ccacacggcc ctcccgtggg
     5761 agtcctccga gccctcgtct tcgagccacc ggcgctcata ggtcatgaag ttccgaaaac
     5821 ggccgttttc ctcgttgtac gcatggtgga cgaaaccgag atagcggcag gcgaggcggg
     5881 tgaggtcctt ttcctcggga atcatttccc gggccagaag gacggctatc aaggcccggg
     5941 cgttgtcatc ggtgcaatag ccgtagatgc ggtcggggac cgtaaacttt gcatgctgca
     6001 ggattcccgt gtcgtctgtg agaagcagga ggtgatcgaa acagggctga ggaagctcgg
     6061 gcggtgcggc atcgagggtt ttggcatgaa atacgggcct cgggtgcttc tcccgctctt
     6121 ttcttacttc actgaagacc tcgaggtatt tccttgcaac ctccttccaa accatgccgc
     6181 ggcaaaaggt gtaggcgcgt tttcgcatgg cgtgccgctc ggtttcgttc tcgaaaaggt
     6241 cgagaatctc gcgtgccagg gccttggagt ctcgaaaggg gaccagcctt ccccgtccct
     6301 cctcgagcat ctcttgggcg taccaatacg cagtggaaac cgttgccttg ccggctccaa
     6361 gagcataggc gagggttccc gaaacgatct gctcgcggtt gaggtagggg gtcacataga
     6421 tatcggcggc acccaggaat tcgcaaagct ccttcaagtc cacaaaccgg ttgtgaaaga
     6481 tgaggtggtc cccgacgccg agctctcggg cgcgccgctg gagggaaagc ctgtaggact
     6541 ctccctgatc gttttttaca tgggggtggg tggcgccaag gaccacgtag gtcgcgtccg
     6601 ggtatcggct cacgatctcc ggcagagcgt ctatcatgtg ctcaatccct tttccgggag
     6661 agagaagacc gaaggtcagc aacacccggc gcccctcgac gccgaactgg tccttgtaga
     6721 aattcggatc gacaaaggga acatccggga tgccgtgagg gataacagcg atcttttcag
     6781 gggcaatgcc gtagacatcg agcagcattg cctcggcctt tctgctcatc accacgaggc
     6841 ggtccgagag cgccccaagc tcgcggatga cgaccttctg ccggggattg ggctccttga
     6901 ggacggtgtg aagagtggtc accacgggca tcctgaggct tcggagaagt tccaggatgt
     6961 gccctccgaa gctcccgccg aatatgccga actcgtgctg gaggcacacg acgtccactt
     7021 ggctcatgtt gaggaagtcg gccgcaagct ggtagtccgc aaggttcttc tggttgagtt
     7081 cgaagcgcac tcgatccgga tacgggtatc cttccggggt gtcgttcatg accatggccc
     7141 agcagctcgt ttccggggcc tcggagctca aggcctcgag cagatcgctc gtgaaggtgg
     7201 caatgccgca ctgccgggga aggtaattgc cgataagcgc aacattgtgc aaattgggag
     7261 gggacaggcc ggctctatcc atgagaccgt ctccaggagt cagatgttca tccggggtgt
     7321 cccgaggctg aagctcaacc tcaagggcag gagaccccac cttccacagg cggagtccga
     7381 tggagcacgg aaaaaaatac cggggctgct gcgggaggga aagctcaagg caccgggaga
     7441 gccacgctgt tgcagccgca gcagtcgatc ttttcgagct cggaacacat gatagccagg
     7501 gcgcagaaaa tgtcaagcaa ttagtctcac aatctaaatg gagagatgca gcatgcaagc
     7561 gccatgcggc tcaaacaagc ggatcaaaca acttgataca aggagagaat gcgcctggcg
     7621 atgagacaat gcccctgcaa tgtggtggat cgggtgctat tttttgcctc gtcgcgatcc
     7681 ggtatcgcgt ctcataaatc aaatgaagag ctccgcagac acaagctgga agatcgaccg
     7741 gaagatccgt cccccacgtc atagagctta tcgagaacct gcctggccat tgaccggctg
     7801 ctataattcc ccccagattt tgggcagcat gttaagctta atggaagttc aaaatcttgt
     7861 tttggagagg ggtaaacag
//
LOCUS       DAOOVC010000170         7811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5643_length_7811_cov_5.697396, whole genome shotgun sequence.
ACCESSION   DAOOVC010000170 DAOOVC010000000
VERSION     DAOOVC010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5643_length_7811_cov_5.697396"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="PLM79_15720"
     CDS             complement(<1..1420)
                     /locus_tag="PLM79_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOV87808.1"
                     /translation="MKLEAMAADLNYPSNFIRNIVAEDRKTGKYGGRVMTRFPPEPNG
                     YLHIGHAKSICLNFGIAAEFGGVCNLRFDDTNPGKEEIEYVDAIMEDVRWLGFDWEDR
                     LYFASDYFERLWEYAVELIKMGKAYVCSLSPEAIRDYRGTLTQPGKDSPYRTRSVEEN
                     LDLFQRMRAGEFEEGAHVLRAKIDMASPNLNLRDPVMYRIRKVPHHRTGTTWCIYPMY
                     DFAHGLSDSIEGITHSICTMEYEDHRPLYDWFLEELGVYHPQQIEFARLNLSYTVMSK
                     RKLLQLVREGHVQGWDDPRMPTIAGMRRRGYPPEAIRNFCERIGVGKKESIVDLALLE
                     YCVREELNKRAPRVMGVLKPLRLVLENYPEGQVEELEAVNNPEDPGAGTRKVPFSRVL
                     YIERDDFMEDPPKKFYRLAPGREVRLRYAYFVRCVDVVKDPQTGEVQELRCTYDPATR
                     GGGAPDGRQVKATLHWVSAAHGV"
     gene            complement(1626..1985)
                     /locus_tag="PLM79_15725"
     CDS             complement(1626..1985)
                     /locus_tag="PLM79_15725"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HOV87809.1"
                     /translation="MIELLDLQLPGVVGMRICGRVEKADILRVARAAEETLSRGFKLK
                     VYVELESLEGVSLEAFVEDLRFALPHLKDFKKKAVVSDKGWLTRLGEMTSRFFPAIEV
                     RHFSWEEKQQAREWVAS"
     gene            complement(2020..3504)
                     /locus_tag="PLM79_15730"
     CDS             complement(2020..3504)
                     /locus_tag="PLM79_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOV87810.1"
                     /translation="MRRMRKCATCFCFHILLLCLWNSTICGQVSGAASDEGRFLCVSD
                     IHFNPFQDDALVPRLMQSGVPTWHGILEKSRERKPSPLGEETNYALLISTLKRMNKVC
                     PTPDFILFSGDTLVHEFEKKFGKYSKSQGELRKFVLKTYEFMSYEFNYFFPRVPIYFT
                     LGNNDGYDGDYEIPPSGSFLHAGADLFRRYWIKEGSSGSPGGFEGPGPGPKVLETGGE
                     AAELGFADTFVKGGNYGVRPVRTRHASLIAINSVYFSSRNSYRSQGRQQLEWLEKQLK
                     DARDGDRKVWILMHILPGIDVRATLSHPGQPTVYWDETTRNSRGRTYLQEFKYLMKKY
                     GPTVRGVFSGHSHMDHFRVVPDADRGRGASAFVHVTPALSPKFRNNAAFQLFRYDRDS
                     MALLDYETHYYHPQYNEWEKEYDFRQTYRRSVLTPGSLDKVREAMIESGYVRSAFIRF
                     YDVNNSKDNPITKTHWKAYWCGIASLTRGEFASCLSGAELPGSP"
     gene            complement(3761..5869)
                     /locus_tag="PLM79_15735"
     CDS             complement(3761..5869)
                     /locus_tag="PLM79_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87811.1"
                     /translation="MEDHRNELELYRRQVDDLTVRNLQLQQELTHAAKTARRSRLLAT
                     LIQRAYRSVGTGGGMDAVGGRFLEVLLDTLSVDLGALFKASDEGHSFVCLHSLGLPPS
                     FVPKLRFSSFPAEFLYADSRTSLDPTAGPLRSALGVPFFLWGFDPPSGFALLLGNASA
                     DRPVHCPFEEGDRELVMGALGVFIDIAARRCAQETLIESEEKYRAFFDNAADCMFTLD
                     LDGSFTDVNKATERFSGYPKDRLVGMNFRDYAAEKDHERIVEALGQILSTEEPLQNFP
                     FEVRLENGRDRFFEVHVVPIKKGQRIVGFQGSARDVTDRKRAEKERGLLESRLQTARE
                     MEAVAKLAGGIAHEFNNALHAITGNVELLELLLPDSDPVAECIEPMKISAERMVRLTG
                     QLLAYARGGKYRPTWMPFGRLLEDTLAILQHSVPSTIRLERDLAADLAAVEVDSTQMQ
                     MVLANILTNAREAIDGVGRIRISAGNVVLDRETARRHPGLAAGLHVFVAIEDNGRGMD
                     QKTLDGLFRPFFSTKFQGRGLGMAAVYGIVKNHQGWIGVESELGKGTSVRILLPALPE
                     RPGEEIPRGEFVRGEATILVVEDEEPLLTMTRRMLEQLGYRMIETRCGRDAVDLVRTY
                     EGDIDLALLDILLPDMPGSRVYDAMKKFRPRMKVIVCSGYALDGAVQEILDAGADGFL
                     QKPCSFRTLSAKLSALLDTP"
     gene            6922..>7811
                     /locus_tag="PLM79_15740"
     CDS             6922..>7811
                     /locus_tag="PLM79_15740"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOV87812.1"
                     /translation="MNDRHTTGFAGPRVGSRTETADLRFGGASSGPSIPFRRPLFWIG
                     LVSFFLLFADLGSYALIEGEAMYAEIAREMRVSGDYITPHLNGTRHFDKPAFLYWLIA
                     LSQGLLGETESAARMWPVLATWGTIFVVAGIGSSLYGSTASRLGALVFAACTGPHIFG
                     RLAMPDGILCFWIALAVLGYVRGYPREGDPTGWRRLMFASLGFAALTKGILGLGLGSA
                     VIGLHVLLGGGLRRFLTWDLVAGVLLTLLVAVPWYGALGIRNPDFFGYFFIREHLLRF
                     TGQRYPRDECLSLPVFLAFT"
BASE COUNT         1434 a         2450 c         2297 g         1630 t
ORIGIN      
        1 ccaccccatg ggctgccgac acccagtgga gagtggcctt cacctgcctt ccgtccggag
       61 cccctccgcc ccgggtggcg ggatcatagg tgcagcgcag ttcctgcact tcccccgttt
      121 gcgggtcttt caccacgtcc acgcagcgga caaaataggc gtaccgaagc cgcacctccc
      181 gtcccggggc cagccggtag aatttcttgg gggggtcttc catgaagtcg tcccgctcga
      241 tatagagcac ccgggaaaac ggcacctttc gggttcccgc gccgggatct tcgggattgt
      301 tgacggcctc gagctcttcc acctgccctt cgggataatt ctcgaggacc agccgcaggg
      361 gtttcaggac ccccatgacc cgcggagccc tcttattcag ctcttcccgc acgcagtact
      421 cgagaagagc caggtccacg atgctttcct tttttcccac cccgatcctt tcgcagaagt
      481 tgcggatggc ctcgggaggg tagcccctcc tgcgcatccc ggcaatggtg ggcatccggg
      541 gatcgtccca tccctgcacg tgaccttccc gtaccagctg cagtagtttc cgcttgctca
      601 tgaccgtgta gctgagattg aggcgggcaa attcgatctg ttggggatga tacaccccca
      661 gttcctccag gaaccagtcg taaaggggcc gatggtcttc atattccatg gtgcagatgg
      721 aatgagtgat cccctcgatg gagtccgaaa gaccgtgggc aaaatcgtac atgggataga
      781 tgcaccaggt ggtgcccgtc cggtgatggg ggaccttgcg tatgcgatac atcacagggt
      841 cccgcaggtt gagattcggg gacgccatgt cgatcttggc ccgcaggacg tgggctccct
      901 cttcgaactc ccccgcccgc attcgctgaa acaaatccag gttttcctcc accgaacgtg
      961 tgcggtaggg actgtctttt cccggctgcg tcagggtccc ccggtaatcc cgaatcgctt
     1021 cgggactcaa actgcacacg taggcctttc ccatcttgat cagctcaacg gcgtattccc
     1081 aaaggcgttc gaagtagtcc gaggcgaaat agagccgatc ttcccagtcg aagcccaacc
     1141 accgcacgtc ctccatgatg gcgtccacgt actcgatttc ttccttgccg gggttggtgt
     1201 cgtcgaagcg cagattgcac accccgccga actccgcggc aataccgaaa ttgaggcaga
     1261 tggattttgc gtgcccgatg tgcagatacc cgttgggctc gggaggaaag cgggtcatca
     1321 ctctcccgcc gtacttgccg gttttcctgt cttcagcaac gatatttcgt atgaaattgg
     1381 acggatagtt cagatcggcg gccatggctt cgagtttcaa tcctccttgc ttcttgatcc
     1441 gagggacgat ctggggcggg ccctccggaa tcggtccctc cggcggccgg ggacgggcac
     1501 gcgccgatcc ctttgccccg gcggaagttt agactcaatc cttcccaaat cacaagccca
     1561 aaaccgcctc cgttgccgtg ccgcccgctt cgagttcttt cgggcccctc aaggagcggt
     1621 ccttctcaag acgccaccca ttcccgagct tgctgttttt cctcccagga aaaatgccgg
     1681 acctcgatgg cgggaaaaaa cctggaggtc atctcaccca gccgggtcag ccaccccttg
     1741 tccgacacca cggctttctt cttgaaatcc ttcaaatggg gcaaggcaaa tcggaggtct
     1801 tctacgaacg cctccaggga tactccttcc agggattcca gttccacata gaccttcagt
     1861 ttgaagcccc ggctcagggt ctcttcggcg gcccgggcaa cccgaaggat gtccgccttc
     1921 tccaccctgc cgcagatcct cattcccaca acgcccggca attgcaggtc caacagttcg
     1981 atcatgtcat ccctccctgc tccgatccat atttctcctt cacgggcttc ccgggagctc
     2041 cgcaccggaa aggcaggacg cgaattcccc gcgagtcaga ctcgcgatcc cgcaccagta
     2101 ggccttccag tgggtcttag tgatgggatt gtccttggaa ttgttcacgt cgtagaagcg
     2161 gatgaaggcg gatcggacat atccgctctc aatcatcgct tccctcacct tgtccaggga
     2221 gccgggcgtc aggaccgacc ggcggtaggt ctgccgaaaa tcgtactctt tttcccactc
     2281 gttgtattgg ggatgatagt agtgggtttc atagtccagg agtgccatgg aatcccgatc
     2341 gtagcgaaac agctgaaacg cggcgttgtt cctaaacttg gggctcaaag ccggggtgac
     2401 gtgcacgaag gccgaggccc cccttcccct gtcggcatcc ggcaccaccc gaaagtggtc
     2461 catgtggctg tgcccggaaa aaaccccccg gaccgtggga ccgtacttct tcatcaggta
     2521 cttgaattcc tgcaggtacg tccgaccccg ggagtttcgt gtggtttcat cccaatagac
     2581 ggtgggttga ccgggatgcg acaacgtggc gcgcacatcg attccgggga gaatatgcat
     2641 gaggatccag acctttcggt cgccgtcccg ggcatccttg agctgctttt caagccactc
     2701 gagttgttgc cggccctggc tgcggtagga gtttcgagag gaaaaataga cgctgttaat
     2761 cgcgatgagg gaggcgtgcc gggtccgaac gggcctcacg ccgtaattgc ctcccttgac
     2821 aaaggtgtcg gcaaaaccca attcggccgc ttcgcccccc gtttcgagga ccttcgggcc
     2881 cggacccggc ccttcgaacc ctcccggcga ccccgaggaa ccttccttga tccagtatcg
     2941 acgaaacagg tccgctccgg catgcagaaa gctcccgctg ggggggatct cgtaatcgcc
     3001 gtcgtatccg tcgttgttgc ccaacgtgaa gtagatgggc accctgggaa agaagtaatt
     3061 gaactcgtag ctcatgaact cgtacgtctt caggacaaat tttctcaatt cgccctgaga
     3121 tttggagtac ttcccgaact tcttttcgaa ctcatggacc agggtgtccc cggaaaagag
     3181 gatgaagtcc ggggtgggac agactttgtt catccgtttc aaggtcgaaa tgagcagcgc
     3241 ataattggtt tcctccccga gggggctggg tttcctttcc cgggacttct cgagaatccc
     3301 gtgccaggtc gggacaccgg attgcatcaa tcgcggaacg agggcgtcat cttgaaacgg
     3361 gttgaaatgg atatccgaaa cgcaaaggaa acgcccctcg tcggatgccg ctcccgacac
     3421 ctgtccgcag atcgtggaat tccacaggca aagcaacagg atgtggaagc aaaaacatgt
     3481 tgcgcatttt ctcattctcc tcatgggact ttcctctcaa gacggggccg ggctgcgtcg
     3541 tgccggggat acaggatctt ccgccttctc ccttcccggg atggggatcg ggccgcacgc
     3601 aatgcttgtg agtgaaggtc cctctccccc agggagaggg ttagggtgag ggaaaatcgg
     3661 ttttgaagcc cccttgtacc ccgtaccctc tccccggaag cgggggagag ggagcgttcg
     3721 tcagtccttg gctcaaagcc accggcctcc ggatcttctc tcaaggcgtg tccaaaaggg
     3781 cgctaagctt ggcggacagg gtccggaaac tgcacggctt ctgaagaaaa ccgtcggcac
     3841 cggcatccag gatttcctgg actgcgccgt ccagggcata gccgctgcac acgatcacct
     3901 tcatccgggg ccggaacttc ttcatggcat cgtagacccg gctccccggc atatccggca
     3961 gaaggatgtc cagcagggcc aggtcgatgt ccccctcgta ggtccgcacc aggtctacgg
     4021 catcccgacc gcaccgggtt tcgatcatgc ggtaacccag ttgttccagc attcgccggg
     4081 tcatggtcaa gagaggttct tcgtcctcca ccaccaggat tgtggcctct cctctcacga
     4141 actcgccccg ggggatttct tctcccggcc gctcaggcag tgcgggcagg aggatgcgga
     4201 ccgaggtccc tttgccgagc tccgactcca ccccgatcca tccctgatgg ttcttcacga
     4261 ttccgtaaac ggccgccatg cccagaccgc gaccctgaaa cttggtactg aaaaagggcc
     4321 tgaacaaccc gtccagggtc ttttgatcca tgcctctgcc gttgtcttcg atggcgacaa
     4381 agacatggag gccggctgcg aggcccggat gtcggcgggc ggtttcccgg tccagcacca
     4441 cgttccccgc gctgattcga atgcggccga ccccatcgat ggcttcccgg gcattggtca
     4501 ggatattggc caacaccatt tgcatttggg tggaatccac ttccacggcg gctaggtccg
     4561 ctgccaggtc cctctccagt cgaatggtgg aaggaacgga atgttgcagg atggccaggg
     4621 tatcttccag gagcctccca aagggcatcc acgtaggccg atacttccct ccccgggcat
     4681 aggcaagcaa ctggccggta agacgcacca tccgttcggc ggaaatcttc atgggttcga
     4741 tgcactcggc cacggggtcg ctatcgggca gaagcagctc gagcagttcc acattgcccg
     4801 taatggcatg cagggcattg ttgaactcat gggcaattcc gcccgccagt ttggcgacgg
     4861 cctccatttc ccgggctgtc tgcagccgac tttccaggag acccctttcc ttctcggccc
     4921 gcttgcgatc ggtgacgtcc cgggcgctgc cctggaaccc aacgatccgc tggcccttct
     4981 tgatgggaac gacgtgaact tcgaaaaacc ggtctcggcc gttctccagg cggacctcga
     5041 acgggaaatt ctgaaggggt tcctccgtgg acaggatttg ccccagggcc tcgacgatcc
     5101 tctcgtggtc cttttcggcc gcgtagtccc ggaaattcat gccgaccagg cgatccttgg
     5161 ggtacccgct gaaccgctcc gtcgccttgt ttacgtcggt gaagctccca tcgaggtcca
     5221 gggtgaacat gcaatctgcg gcgttgtcaa aaaacgcccg gtacttttcc tcactttcga
     5281 tcagcgtctc ttgggcacat ctgcgggcgg caatgtcaat gaagaccccc agggctccca
     5341 tgacgagctc ccggtcccct tcttcgaaag ggcaatggac gggccggtcg gcggaggcgt
     5401 tgcccaggag cagcgcaaat ccgctgggcg gatcaaaccc ccacagaaag aacgggaccc
     5461 cgagtgccga gcgcagggga cccgccgtcg ggtcgaggga agtccgggaa tccgcgtaga
     5521 ggaattcggc cgggaacgac gagaaacgga gcttcgggac aaaggaggga ggaagcccca
     5581 gggagtgcag gcagacaaag ctgtggccct cgtccgaggc cttgaagagg gcgccgagat
     5641 cgacggacag agtgtccagg aggacctcca ggaaacgtcc tccgacggcg tccatgcccc
     5701 ccccggtgcc gacggaccga taggcccttt ggatcagggt cgccagcagc cggctgcggc
     5761 gcgccgtttt tgccgcatgg gtcagttcct gttgcagttg gaggtttcgc acggtcaggt
     5821 cgtcgacctg gcgccggtaa agctccagtt cattccggtg gtcctccatc acacttcctc
     5881 gatgcaaaaa tccgtaagga cccccgctca atccgggagg cgggcgaggc ccggcaaggg
     5941 gccgccgcaa catcggggtc aaaccccgcc gggggggacc ccgggacccg gctcctcatg
     6001 caatgcaccg gcccctttga cttgggaacc gctgaaggcg gagaccctcc cggcacaatc
     6061 gatgcgcaga gccgggagcg gtgaggaagg acccggggaa tcgagaaact tccgtatccc
     6121 tcgccggacg gactcgagca cgagctgatg ctcgggggtc atgggctgag cttttgctcc
     6181 ttgggcgatc tccccaatgg agaatgcatc gcagccctta tcaaaaccga tggatatcac
     6241 gggcactctt tcaaggaggg ccgttcacgg atggagagct cttcctccca ccttgcttac
     6301 ccgggaggaa aaccgccggc tctcggcacc agaattacca catttccccc gcgaccacaa
     6361 aaggggaata ctgcctcccg gggccgtgcc gcccatccgt gcttcgtagc cgggaacccg
     6421 gtgccccgcc cgcccgatgc cggtgaagga aggattccct tccctcaggg agaggggtga
     6481 gggaagatcg gccctccaag tcccaccaac cctctccccc gcttccggga agagggaggc
     6541 gatgtgggac cttacttcaa atcacgttga cccgccgtcc ggtcgcggag aagccggaag
     6601 ggcatcttcc cggctttgga tcgaggggga gggacgagac ggcccatcat ggctcctctc
     6661 gatccggagg attcggggcg gacgcattcg ttttttctct caagcgggaa gatttatggt
     6721 actacgcctt acgattgttc cgaggttgag tcgcattcgg gatcgacggt ccggcccacg
     6781 agattcctgg ggccggggga gccgaacggg gtcggttccc cggccttgat ctcaatttcc
     6841 gttgccggac gcctgtttct tgcttggatt cggcgtgccc tcctcgtttt gggaagggcc
     6901 gctttcaagg tacgaacaac gatgaatgat cgccacacaa ccgggtttgc cggtcctcga
     6961 gtcggttccc ggacggaaac ggcggacctc cggttcgggg gtgcctcttc cgggccttcg
     7021 atcccgtttc gtcgccctct cttctggatc gggctcgtct ccttctttct cctgtttgcc
     7081 gatctgggct cctacgccct catcgaaggc gaagccatgt acgccgagat cgcccgggaa
     7141 atgcgcgtga gcggggacta catcacgccc catctcaacg ggacccgcca tttcgacaag
     7201 ccggccttcc tttactggct gatcgccctg agtcagggcc tgctggggga aacggaatcg
     7261 gccgcccgca tgtggccggt tctggccacc tggggaacca ttttcgtggt ggccgggatc
     7321 ggcagctctc tttacgggag taccgcatcc cggctgggcg ctctggtctt tgccgcctgc
     7381 acggggcctc acatcttcgg gcgtttggcc atgcccgacg gcattttgtg cttttggatc
     7441 gccctggccg ttttggggta cgtgcggggg tatccccggg agggggatcc aaccgggtgg
     7501 cgccggctga tgttcgcaag cctgggattt gccgccctga ccaaggggat cctgggattg
     7561 ggcctgggat ccgccgtgat cggacttcac gttcttcttg gcgggggcct gcgccgtttt
     7621 ctcacctggg acctggtcgc gggcgttctg ctcaccctgc tcgtcgccgt cccgtggtac
     7681 ggggccctgg ggatccgaaa tcccgacttt ttcggctatt ttttcattcg cgagcacctt
     7741 ctgcgtttca cgggccagag gtatccccgg gacgagtgcc tttccctacc ggtgtttctg
     7801 gccttcacct a
//
LOCUS       DAOOVC010000171         7799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5657_length_7799_cov_4.024664, whole genome shotgun sequence.
ACCESSION   DAOOVC010000171 DAOOVC010000000
VERSION     DAOOVC010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7799
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5657_length_7799_cov_4.024664"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLM79_15745"
     CDS             complement(<1..383)
                     /locus_tag="PLM79_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOV87813.1"
                     /translation="MADRRLLNAAIQEIRGNLEQLEAVFEGGHCVVLAGPGSGKTKTL
                     TIAMARALLEDVREPRGIACITYNNECAIELETRLSRLGVEPGERVFIGTILSFALTQ
                     VILPYARCAMPEIATGIRVANRSECR"
     gene            complement(376..2169)
                     /locus_tag="PLM79_15750"
     CDS             complement(376..2169)
                     /locus_tag="PLM79_15750"
                     /inference="COORDINATES: protein motif:HMM:NF022840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87814.1"
                     /translation="MYLSRVQIKNFRNFADLDVSLDANIVIVGENRVGKSNFIFALRL
                     VLDNSLPDSARQLKLSDVWDGYDGSASDDGPQIEIHLDFTDFDDDDDLTALLTDYRLA
                     DDHTIARLSYVFRKKDGVEGCPISEADFDFKVFGGADETRAVSPQVRRRICLDVLHAL
                     RDAESDLSTWRNSPLRPLLEDAISQVPEEDLTAVSKAMNKATTQMAKLDPIRELEDEL
                     RKRMAELAGANQDLDAKLAFAPTDPLRLFRAIRVFIDGGKRGISEASLGSANLALLTL
                     KLAEFEWRRQKNERNFTLVAVEEPEAHLHPHLQRKVFSSLFEENEQDGQSLILSTHSP
                     NIASVVPLGSIVLLKDEGSRGTRAYSLANLSLKGEDLEDLQRYLDVTRAEVLFSRGVI
                     FVEGDAEAALLPVFAPSLGHDLDQLGITICNVCGTNFTPYVRLAAALSLPFSVVTDWD
                     PRADGKKAYGWNRSLKLIRAIRDARTDEPLPPRRAQRLRDEERHLRITAERHGIFLNG
                     DTLETEIARTPSLVEPLLNVLREQEFGLRLTERIDEWLEDNSKIDNSQLMLMIGYVSK
                     GRFAERLTEMASGLEPPKYIKLAIEHVVNNG"
     gene            complement(2340..4136)
                     /locus_tag="PLM79_15755"
     CDS             complement(2340..4136)
                     /locus_tag="PLM79_15755"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87815.1"
                     /translation="MEFQRGLNIICGASDTGKSFLVEAVDFLLGGGDPLRDIPARVGY
                     DRARLILEQADGDTFTIERSTSGGGFRRFDGIWLSEDPEANAVILREKHAKGRRDTLS
                     ALLLSIVGLEEQIVRRNKAGETRTLSFRDLARLVIVQEGEIIKRFSPIFSGQWIQKTV
                     ENSVFKLLLTGVDDSAVVGQADKSHTKQGNSSKVELIDEWIAELYAEIDSHGIRREEA
                     EEQLAHLTATIERQRESLKKLRDKLDDRLARRRDILLELEGMSRRIEEVGDLFSRFAL
                     LKTHYNVDLERLAAIEESGCLFMHLDRKPCPLCGALPEMQHQDDACAGNIETVVHAAT
                     AEIAKIRRLLIELDQTVADLTSEAAELNIRKNQLNAQFSELDREIRETISPDLGDAQS
                     AFSELVEKTGEIRHVVDMYERSERLEGQKRDLLETTEESAEIDIPPYIDLSKSVLDDF
                     ATKVKDLLNTWGFPGCDRVYFDEASADIVINGQPRASRGKGLRAITHAAMTIGLMEFC
                     KERALPHPGFVVLDSPLLAYWAPEGPADSLQGSDLKERFYEYLADTHRDSQVIIIENE
                     HPQASLHGRIGLTVFTKNPQEGRYGFFPFVDE"
     gene            complement(4202..4681)
                     /locus_tag="PLM79_15760"
     CDS             complement(4202..4681)
                     /locus_tag="PLM79_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87816.1"
                     /translation="MITPFNSALETGVRSLAILNAVFPDALDLQRLMDFDYLVVHSGD
                     IGGPESLHAPLPLRSGELLVRRGLIEKGLLLMMSRGLVQQITSAIGIQYVASDSAGAF
                     LSALSSTYITRLKECAEWVAVTFSETTDEELHAITRRFFDEWSTQFQAVERPREERQ"
     gene            complement(4683..5786)
                     /locus_tag="PLM79_15765"
     CDS             complement(4683..5786)
                     /locus_tag="PLM79_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87817.1"
                     /translation="MISSDDMKPISPRVAMACSSAEHVSFGVPIPKVIRVATFSPDDW
                     ECFVEEWATSLEDTYVHVARFGGSGDLGVDVVGFVSDRTFNGGWENYQCKRYENPLRP
                     SDIWVEIGKIVYYSFKDEYPPPRKHLFVASRDVGTSLQKLLGDPEKLKEQTKANWGKH
                     CMGEITSTASIPLEGVLLDYFERFDFSIFSAKSVVELIAGHAKTSFHSVRFGGGLPPR
                     PAAAEPPSEHEAIESRYIQQLFEAYEDHLGRSVHKVSDLSAEPKLARDFLRQRERFYH
                     AESLRNFARDTVPEGTFERLQDEIYYSVIDTCEADHHDGFERMRATVAQAAQLPTTSN
                     PLASVILVQDRQGVCHQLANADRLSWVFKDHEV"
     gene            complement(5955..7322)
                     /locus_tag="PLM79_15770"
     CDS             complement(5955..7322)
                     /locus_tag="PLM79_15770"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87818.1"
                     /translation="MDGKDLREGETLELKECWTPRALEDLVAFANQSGGTLLVGVQDD
                     GEVLGFEGNDADLQRIASQIVDSLGLTPSLSVQEYRGKSVLEIHIGPATIPVPFKGRY
                     LTRVGSTNRDMTPEQIARRLMARLGQSWDSLLSTKSLDDVNEQTVRQFLQLARQRLSH
                     LSENEPTHRILDNLNLLDNDRLTNAGVLLFGAQPQRVFPASQIHLGQFRNKVITDDRT
                     LQGDLFGLLKAVMERLQYYLAVRFDVAVAGASLEELQRHETWEYPLDALREAVINALI
                     HRDYAALGEIQIRVHEDRLDIWNPGTLPEGISIDDLRREGHVSKLRNPLLAQTFYYAG
                     LVERWGTGTTRMIAVCRAHGLPEPQFLEESNGFKVTFCKDVYGAERLGKMGLNERQIQ
                     AVLYVKGKGSIGNTEYQKLTGASKPTATRDLKDLQQRGILILRGTGRTVHYVLKDSKA
                     AQRDH"
BASE COUNT         1816 a         2155 c         1894 g         1934 t
ORIGIN      
        1 cgacattcgc tcctgttcgc aacgcggatg ccagttgcaa tctccggcat cgcgcaacgg
       61 gcgtagggca agatgacttg ggtaagcgca aagctgagga tggtaccgat aaataccctt
      121 tcgcccggtt ccacgcctaa tctgctcagt cgagtttcca actcgatggc gcattcattg
      181 ttgtatgtga tacaagcgat accccttggt tctcggacat cttcaagaag agcccgtgcc
      241 atagcgattg tcagtgtttt cgttttgccg cttccgggac ccgccaatac gacgcagtgc
      301 cctccttcga acacagcctc cagttgctct aggttccctc tgatctcctg aattgcggca
      361 ttcagtaatc ggcggtcagc cattgttcac cacatgttcg atcgccagtt taatgtattt
      421 cggcggctcc aggccactgg ccatttctgt caatcgttca gcgaaccggc ctttgctcac
      481 atagccgatc atcagcatta gctgggaatt gtcgatcttc gagttatctt ccagccattc
      541 atcaatgcgt tcagtgagcc taaggccgaa ttcctgctcc ctcagtacat tcagcaaagg
      601 ctcaaccaat gaaggcgtcc tcgcgatttc agtttccaac gtatccccgt tgaggaagat
      661 accatgcctt tcagcggtta tccgcaggtg tctttcttca tccctgagcc tttgcgctct
      721 tctgggtgga agaggctcat cagttcgcgc gtccctgatc gctctgatca gcttgagact
      781 tctgttccaa ccataagcct tcttcccatc cgcacgtgga tcccagtcag tcacaactga
      841 aaacggcagc gacaaagctg cagcaaggcg cacataaggg gtgaaattgg tcccgcaaac
      901 attacagatg gtgataccga gttgatccag atcatggcca agactcggtg caaatacggg
      961 gagcagagcc gcttcagcgt caccttctac gaagatcacc ccgcgggaaa acagcacttc
     1021 agcgcgggta acatcgagat agcgttgcag atcctccagg tcttcacctt tcagagagag
     1081 atttgccagg gaatacgccc tggtacccct gctaccctcg tctttcaata gcacgatcga
     1141 accgaggggc accacactgg cgatattcgg tgaatgtgtg gacaagatca agctctggcc
     1201 atcttgttca ttttcttcga aaagactgct aaagaccttg cgctgcaaat gagggtgcag
     1261 atgcgcctcg ggctcttcaa ccgcaacgag agtgaagtta cgctcattct tctggcgtcg
     1321 ccattcaaac tcagcaagct taagcgtgag tagtgcaagg ttggcagaac cgaggctggc
     1381 ctcactgatc cctcgcttgc caccatcaat gaagactcgg atcgccctga atagtcggag
     1441 cggatcagtt ggtgcgaagg ccaactttgc atcgagatct tggttggctc ccgcaagctc
     1501 ggccatcctt ttccgcagtt cgtcctcaag ctcgcggatt ggatcgagct tcgccatttg
     1561 tgttgttgcc ttgttcatgg cttttgaaac ggctgtgagg tcttcttctg gcacttgact
     1621 gatggcatct tccaaaagag gacgtagggg tgagttgcgc catgtgctga gatcgctttc
     1681 ggcatcccga agcgcatgaa gtacatccag gcaaatgcgc cggcggacct gagggctaac
     1741 cgctcgagtt tcgtcagctc ccccaaagac tttgaagtcg aaatcggcct ctgatatggg
     1801 gcagccttcg actccatcct tctttcgaaa gacgtagctg agtctcgcaa tcgtgtgatc
     1861 gtccgccagc ctgtagtccg tcagcagagc ggtgagatcg tcatcgtcgt cgaagtcagt
     1921 gaaatcgagg tggatttcaa tttgcgggcc atcatcgctg gcgctgccat cataaccgtc
     1981 ccatacatcg ctcagcttga gttgccgagc gctgtccggg agagagttgt cgagtaccaa
     2041 acgaagagcg aagatgaaat tgctttttcc tactcggttc tcacccacta tgacaatgtt
     2101 tgcatccaac gacacatcaa gatctgcgaa gtttctaaaa ttctttatct gaaccctgct
     2161 caagtacatg gttaccccct ccggttccat gccctgtcag actaccatca acatcaaaga
     2221 atcgggctat gtttattgtc ttgtcgtcat cttttcatga aatctgtcaa agcgttcccg
     2281 caaaatccat tgacggcatc gaaggtttta agatcaagct aattcacctt tttcctgcat
     2341 cattcatcaa caaaagggaa gaacccatag cggccctctt gagggttctt cgtgaaaaca
     2401 gtaaggccga tcctgccatg tagactagcc tgtggatgct cattctcaat gatgatgacc
     2461 tgactgtctc tgtgagtgtc agccaagtac tcatagaacc gctctttcaa gtccgatcct
     2521 tgcaagctgt cggcagggcc ttccggagcc caataggcca gcagaggaga gtccaggacg
     2581 acaaagcccg ggtgcggcag cgctctctct ttgcagaatt ccattaaccc gatagtcatt
     2641 gcggcatggg ttatcgcacg caagcccttg cctctgctcg ctctgggctg cccgtttatt
     2701 acgatgtcag ctgaagcctc atcgaaatag acccggtcac aaccgggaaa gccccaggta
     2761 ttcaagaggt ctttgacctt cgtcgcaaag tcgtcaagca ccgactttga gagatcaatg
     2821 taaggtggta tgtcaatctc tgccgattct tctgttgtct ccaggagatc tcttttctgc
     2881 ccttcgagtc gttcagacct ctcatacatg tcaacgacat gccgtatttc accagtcttc
     2941 tcaaccagct cgctaaaagc ggattgagca tcaccaaggt ccggggatat ggtttctcgt
     3001 atctcacggt cgagttcact gaattgagca ttgagctgat tctttcggat attcagctcc
     3061 gcagcctcag atgtcaaatc cgccactgtt tgatcaagct ctattaagag cctcctgatt
     3121 ttggcgattt ctgcagtagc ggcatgaact acggtttcta tgttaccggc acaggcatcg
     3181 tcttgatgtt gcatttcggg cagagccccg caaagtggac agggttttct atccaggtgc
     3241 atgaagaggc acccggactc ttcgatggcg gcaagacgct caagatcaac attgtagtgg
     3301 gtcttcagga gagcaaatcg tgaaaagaga tcgccgacct cctcaattcg tcgggacatc
     3361 ccctccaatt ctaacagaat gtcccgtcgg cgggcgagcc tgtcatctag tttgtctcga
     3421 agcttcttca ggctctcgcg ctggcgctcg atggtggccg taagatgcgc caactgctct
     3481 tccgcctctt ctcgcctgat cccatggcta tctatctcag cgtacaattc cgcaatccac
     3541 tcatcaataa gttccacctt tgaggaatta ccttgcttag tatgagattt gtccgcctgg
     3601 ccgacaaccg cactatcatc aacaccggtg agcagcagct tgaaaactga attctcgact
     3661 gtcttctgaa tccattgtcc cgaaaagatc ggtgaaaagc gcttgattat ctcgccctcc
     3721 tgaactatca ccaaccgagc caagtcccgg aaactgagag ttcgagtttc acctgctttg
     3781 tttcttctga caatctgctc ttcgagaccg acaattgaca gcaatagggc cgatagtgtg
     3841 tcgcgtctgc cctttgcatg tttttcccgg aggatgaccg cattggcttc cggatcttcc
     3901 gacagccaga ttccatcaaa ccggcgaaag cctccaccgc ttgtacttct ttcgatggtg
     3961 aaggtgtcgc catctgcctg ctccaatatt agtctcgccc ggtcataccc aacacgtgca
     4021 gggatgtctc taagcggatc acctccaccg agcaaaaaat ctaccgcttc aacaagaaat
     4081 gacttccctg tgtccgacgc tccacaaatg atgttcaggc cacgctgaaa ctccaagcag
     4141 gcgggttcct cgacacgagg tccggtaaag gaaataccgc gtagttgtaa ggcactccat
     4201 gtcattgtct ctcctcccgc gggcgttcaa ccgcctgaaa ctgggtggac cactcgtcaa
     4261 aaaaacgtcg cgtaatcgca tgcagttctt cgtcggttgt ttcgctgaag gtgacagcca
     4321 cccattcagc gcattctttg agcctcgtaa tgtatgtgga actgagtgct gaaaggaagg
     4381 caccagcact atcagaggca acgtattgga taccgatcgc cgaagttatt tgctgcacca
     4441 gtcctctgct catcatcagc agaagacctt tctcgattaa accacgccgc accaagagct
     4501 ccccagaacg gagtgggagt ggagcgtgaa ggctttcagg cccacctata tctcccgaat
     4561 gcaccacaag ataatcgaag tccatcagac gctggaggtc gagtgcatcc ggaaacacgg
     4621 cattcaatat agcaagagat ctaacgccag tctccagagc actgttaaac ggcgttatca
     4681 tttcacacct cgtggtcctt gaagacccag gaaagcctat ctgcattggc caactgatga
     4741 caaacgccct gacggtcctg gaccagaatt acactcgcaa gcggatttga cgttgtcgga
     4801 agttgagccg cttgagcgac cgttgcacgc atgcgttcaa aaccgtcgtg atggtcagcc
     4861 tcgcaggtgt cgatcacact gtaataaatc tcatcttgta gcctttcgaa cgtcccctcg
     4921 ggtaccgtgt cacgagcgaa gttgcgcaga gactctgcat ggtagaaacg ttcccgctgc
     4981 ctcagaaaat cgcgagccag ttttggctca gccgacaagt ccgagacttt atgcactgac
     5041 cttcccagat gatcttcata cgcttcgaat agctgctgaa tatagcggct ttcgattgcc
     5101 tcgtgctcgc tcggcggttc cgctgctgca ggcctgggag gaaggcctcc gccgaaccgg
     5161 acagagtgaa aagatgtctt ggcatgacca gctataagtt ccacaacaga cttcgcagaa
     5221 aaaatggaga agtcgaacct ttcaaagtaa tcgaggagca ccccctccag tggaatgctg
     5281 gcggttgagg taatctctcc catacagtgc ttgccccagt tagcctttgt ctgctccttg
     5341 agcttttccg gatctccgag caacttttgt agggaggtgc ccacatcacg tgaggcgacg
     5401 aaaagatgct ttcttggtgg aggatattca tctttgaacg aataataaac gatttttccg
     5461 atttccaccc agatgtcgct tggacgaaga gggttctcgt agcgcttgca ctggtaattc
     5521 tcccaccctc cattgaaagt cctgtcgctg acgaatccaa caacatcgac cccaaggtct
     5581 ccggagccgc cgaaccgtgc gacatgcaca taggtatctt ccagtgaagt tgcccattcc
     5641 tcgacgaaac actcccaatc atccggggag aacgtagcga cacgaattac cttgggaatc
     5701 ggaacaccaa aagaaacatg ttcggccgag gaacacgcca tggccaccct tggcgaaatt
     5761 ggtttcatgt cgtcagagct aatcataggt cccggatccc attctgtttc actggcatgt
     5821 aagcatctct cctgtgagct ggcaacacgg gatatccata aacggtaagc cgtgtcgagg
     5881 ccatcttgtt tgagtcaaag gattggcaca aagatgatgc tcgtgcctga tccctttgag
     5941 ccgtaaatga gccgctaatg atccctttga gccgctttcg agtcctttaa aacataatgg
     6001 acagttcgcc ccgtccctct cagaataaga attccgcgct gctgaaggtc cttcaggtcg
     6061 cgcgtagcgg tgggtttgga tgcgccggta agcttctgat attccgtgtt ccctatgctg
     6121 ccctttcctt ttacatagag taccgcctgg atctgccgct cattgagacc catctttcca
     6181 agtctctcgg cgccataaac atctttgcaa aaagtaacct tgaagccgtt cgattcttcg
     6241 agaaactgag gctcaggcag tccatgagcg cggcacactg cgatcatgcg tgtggttccg
     6301 gtgccccacc gttcaaccag gccggcgtaa taaaatgtct gtgccagaag aggattgcgc
     6361 agcttggaga cgtgtccctc ccggcgcaaa tcatcgattg aaattccctc gggcaacgtg
     6421 ccagggttcc agatatcgag ccggtcctca tgaacccgga tctgtatctc tccgagggct
     6481 gcataatctc tgtggatcaa ggcattgatg accgcctcgc ggagtgcgtc cagggggtat
     6541 tcccatgttt catgtctttg gagctcttcc aggctggccc cagcgacagc gacatcaaat
     6601 ctcacagcga gatagtattg aagcctttcc ataacggcct tcaacaaacc gaacagatct
     6661 ccctgcaaag tcctgtcgtc tgtgatgact ttgttcctga attgtcccag atggatttgg
     6721 gatgctggga aaaccctctg aggttgagct ccgaacagga gtactccagc attagtaagg
     6781 cgatcattgt ccaggagatt gagattgtcc aggatccgat gtgttggttc gttttcgctg
     6841 aggtgagaga gtctctgcct ggcaagctgg agaaactgcc ggacagtctg ctcattcaca
     6901 tcatcaaggc tttttgtgga cagaaggcta tcccaactct gtccgagcct cgccatgagg
     6961 cgacgggcga tctgctcggg agtcatatct cggttggtac tcccgacgcg cgtcaagtat
     7021 cgccccttga aagggacggg gatagttgcg ggaccaatat ggatctcgag gaccgacttg
     7081 cctctgtact cctggacaga aagactgggc gtcaacccga ggctatcgac aatttggcta
     7141 gcgatgcgct ggaggtcggc atcgttccct tcaaagccca acacctcgcc atcatcctga
     7201 actcccacca atagcgttcc tccagattga ttggcgaaag caaccaggtc ttccagggct
     7261 ctcggcgtcc agcactcctt cagctccagg gtttctccct ctcgaagatc cttgccatcc
     7321 atacgaaata gactccagtt tctgtttctc tcattcccgg cgccacaaaa gccacaggtt
     7381 tcataccatg tttccgcggt ccatgtaatg catctttacc gaatcatgtc caaggtggca
     7441 gtcttcgccc gtcgacgcat cggtcctttc agtgaagtcg aacaggcgca ctttgaagct
     7501 ggaaaaactc gaacccggtt tgcatcggaa agaaattgtc attcttcctg aaatgccata
     7561 atatcaaagc gatacacaat cggaagtttt gccataattt tgccaaaacg gtgaaagaca
     7621 tcggtaaaga acggtaaaga tagtgaagat cagcggatgg gtgagaatgg ataagtcctt
     7681 gttttgccta aaaaacgtta cctatgttta cccttgttta cttatggcag agggtgattc
     7741 accaattcgt aatcagcagg tcgcgggttc gaatcccatc gccggctcca attcaatcc
//
LOCUS       DAOOVC010000172         7787 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5667_length_7787_cov_6.772763, whole genome shotgun sequence.
ACCESSION   DAOOVC010000172 DAOOVC010000000
VERSION     DAOOVC010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5667_length_7787_cov_6.772763"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..357)
                     /locus_tag="PLM79_15775"
     CDS             complement(106..357)
                     /locus_tag="PLM79_15775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV87819.1"
                     /translation="MPIYEFRCTQCGEVREFLVSGSSDETEMRCEKCGGEELERVLSR
                     VSYVVGSGGGKGAQPTAVTKSCGPGKSCTSFELPGHSKS"
     gene            530..1453
                     /locus_tag="PLM79_15780"
     CDS             530..1453
                     /locus_tag="PLM79_15780"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV87820.1"
                     /translation="MKERQALAPDDVPDNPAFWDWEQLHRQWLNRIQAILKGSDSRVP
                     PTRVRHQVEQTVAYREIVDRWPESSPSDRLRAWKTLLVLTEESSTRVLPVCVQCGECC
                     RKSSPSLYLQDLDLLREGKIPWDKLYTIRRGEPVRSPDRDRLFHLLDERVKIQEKTGS
                     GECVFLDSIHSLCTIYPDRPLQCRAQVCWDPDVAAQFANEPYLTRRDLFQGVEILADI
                     LAEHDRRCGYEKLRAAFQRLGESKGDTIEEVLDLLGYESHFREFFSEKLSIPPTMLPL
                     VFGRSFGDLVVHFGFRVREDTDGSRILLPDP"
     gene            2198..>4767
                     /locus_tag="PLM79_15785"
     CDS             2198..>4767
                     /locus_tag="PLM79_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87821.1"
                     /translation="MKKLLFAFIVMICLLSPIPSSTLADRCSAPAFVAASAPPLVMFV
                     MERDHRLYYEAYNDASDLDEDGMLDVGYKHSIDYFGYFDSYKCYTYVSTGTRRFVPAR
                     VTPNKYCGGAEEWSGNFLNWLSMSRMDVLRKVLYGGTRVTDSSSETVLEGVYIPQDAH
                     SWGKEYAGADTRDLTPWDPPTSPNRHLFCMTSTSEGSPHLIRVARNDSHRIWEWASTE
                     RAVCGGPDTPAGYRQAPVATRGDILDYIVRVQVCNGSVGNEPNCKEYPGGGGVRKPTG
                     LLQIYAEGDGTKVCSKTWKSCNTDSGCGATEGLCVDKTQMYLGLITGSYVKNLSGGVL
                     RKNIWGVLDEINAQSGSFQSSENTQGNIILTLDRMKTVGFRYSDYSYQGTAGGTCGWI
                     TDRPINEGECRMWGNPIGEMMYEALRYFTEKTSPTGNFTYSTTQDAGLSLSKPSWGMP
                     KGSSTLTLYNIFPSCAKGFLLVLSDVNNSYDSDQVPGAYSTFAASTFTGDFPGLDVTA
                     LLNDIGTRESIPGGNYFVGQSQSDFDFICSSKSVSNLSSIRGLCPEEPTKQGHFSPAA
                     MAYYGKSFMTIQTGKPDVTTLAVAMSSAVPDIKVKVGDQSVRIAPVGKSVSGDYNVYT
                     KCAANCTLVRDVNGLHISNCNANAYCPSNQIVDFYVEQITYDADKNLTNATFRVNFED
                     VEQGADHDMDAIVMYQVQPVGSNQVQVTVTSSYAAGSIDQVLGFTISGTSEDGLYLVV
                     RDRDMCGSGTGTCSDANTPAAVGNLPLEWTKTFTVVGNPAGLLKNPLWYAAKYGGFSD
                     SNGNRIPDLTPEWDKDGDGVPDNYFFVDNPLKLYEQMERAFIAILNRGGSAGAVATVT
                     "
     assembly_gap    4768..4867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4868..7539
                     /locus_tag="PLM79_15790"
     CDS             <4868..7539
                     /locus_tag="PLM79_15790"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HOV87822.1"
                     /translation="IAILNRGGSAGAVATVTQQVEGEDIVMRGAFTSYELDPNHFVWK
                     GHLESYWPYGGCSDYLTQSRCEELVGCKWQGSSCTGSLYSFQREERLGQFCFEHHDHC
                     WTAEDHLPTPNNRTIYSMINGSQVAFTSANVCTASDWLNLAGDSLFSTTDCADLVSWV
                     RGTDTWTKARDRDDWILGDIVFSTPVVVQSPSLAAVPSAAVGDCAQTAGHPCSGGCDC
                     SSDCVETCYYCFRECNKTRKKVVYVGGNDGMLHAVVAGIWYSDPNPNVDGDGDGVKDE
                     SHWIYDPNAPNSECDGQVCTGKDQIGKELWAYIPSNILSELKELARPTYGTQGGCKHR
                     FLVDLSPQAWEVYIDPDGNGRRWRTVLLGGERDGGDMLFAIDVTDPDQPKILWEYPIL
                     RNMVQITASGSDYLATLPFLDKTVYDQVKNLPASWSIPYVGKLKIPTNVSFLAANAIP
                     SWTAGTPALTLSNRGPNDLSGWVAIIGDTSRVFNQAELPGTLTAAQKDAALKPHLLAI
                     DIETGVNLFQYLWPMLHGALPAQWPKTVSGTNVVPHSMTSPVVLDLWDSTGHLNPDGY
                     LDHIYLGDLNGNFYGMKFNLDEGTTLGMSMDVWKTKPVAATDVGSNNYRSSREPISVQ
                     PSTALDQDQNVRIYFGTGKFDNLEGTSNDKTDTAKMSFYNLVDSSQRPSITAASSVTK
                     ISDSPTRFTASGGIASGFKMYNFGIDINLHCSSPTYNASCTWVKSDGTQDCCESACAS
                     PANPCWDCIYGLNFPGERVVDSALVAGGLVFFTTFIPKSEPCSAGGDSYFYVLDYQCG
                     SLTTNPFGQSNFVLRSSVQDLTNPGDYAAVTEGTKTVAYVAKLGPGMPSRPVLDSSGE
                     YVFIQTSDAQIHKIKVELLFQPFELRGWKEEPQ"
     gene            complement(7573..>7787)
                     /locus_tag="PLM79_15795"
     CDS             complement(7573..>7787)
                     /locus_tag="PLM79_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87823.1"
                     /translation="QSMGQGTITPRIDYLGYRTTPPPGWMLNWQGYSSFHGLYYRPVG
                     QGSVPLPSGKGNAQSTVGAVTLKVAR"
BASE COUNT         1668 a         2320 c         2186 g         1513 t
ORIGIN      
        1 aatccacccg ggaaagaggg gttgagctca ccccgctaat tcaacatcct gctaagggaa
       61 caggcctcct tgtcctccgt cgctgcagac ggctccttcg ttgaatcagg acttgctgtg
      121 accgggaagc tcgaaactcg tgcagctctt ccccggaccg caactcttgg tcacggccgt
      181 gggctgggcg cccttgccgc cgccggatcc cacgacatag ctcacacggc tcaatacccg
      241 ctccagttcc tctccgccgc acttttcgca gcgcatctcc gtttcgtccg acgaaccgga
      301 aaccaggaat tcacggacct ctccgcactg ggtgcagcgg aattcgtata tcggcatgtt
      361 gtcccctttc ggaccattga gagtgaatcg caaatttgca ggggttttct aacaaatggt
      421 gcccgacctg tcaatgttgg gtgccgagct cgagccatcc cgggattggt caatcggagg
      481 aaattgtgtt ttaatgatca ctcccatgga gaaaggagta ggaatcccta tgaaagaacg
      541 tcaggccctg gcaccggacg atgtcccgga caatccggca ttttgggact gggagcaatt
      601 gcaccgacaa tggctgaacc ggatccaggc catcctcaaa ggctccgatt cccgagttcc
      661 ccccacgagg gttcgccacc aggtggaaca aacggttgcc taccgggaga tcgtggatcg
      721 atggccggag tcttccccat cggaccgact ccgtgcctgg aaaacccttc tggttttgac
      781 ggaagaaagt tccacccggg tgctgcccgt ctgcgtccaa tgtggtgaat gctgcaggaa
      841 aagcagcccg tctctctatc tgcaggacct ggatctgctg cgggaaggca agatcccctg
      901 ggataagctg tacaccattc gtcgaggcga acccgtgcgt tcgccggacc gggaccggct
      961 gtttcatctc ctcgatgaac gggtgaagat ccaagaaaaa acgggaagcg gagaatgcgt
     1021 cttcctggac tcgatccata gtctttgcac catctacccg gaccgtcctc ttcaatgccg
     1081 ggcccaggtc tgttgggacc ccgacgtcgc cgctcaattc gccaacgagc cctacttgac
     1141 gagaagagac ctgtttcaag gagtcgaaat ccttgcggac atcttggccg aacatgaccg
     1201 gcgatgcggg tacgagaagc tgcgggctgc ctttcagcga ctgggagaga gcaaggggga
     1261 tacgattgag gaagtccttg acctgctggg atatgaaagc cactttcggg agtttttctc
     1321 ggaaaagctg tccattccgc ccaccatgct cccgctggtc tttggaagga gcttcgggga
     1381 cctggtggtt cattttggct ttcgggttcg ggaagacacg gacggaagcc gaattctgct
     1441 tccggaccct tgacccccgg gacccgggcg accctgccgg gcgcacaaaa aaataagcta
     1501 accggagggg ctagggccgg ttagcttttg aggaggaaag agttggtttc tttcacaaag
     1561 cacggagttg caagaaaagt accaacttcg acttgattcg cctccggtgt ggtccgtctt
     1621 tgggcggcgc gggattccgg cccgatggac cccggtttct cgggaaagaa acgaaggctt
     1681 cgcggggaaa tcctgccata atcccgccat actcacgcca tccaatcccg gaagccggcg
     1741 caattcaccg cccgagaaag ggttcatcgt attcctgtaa tctcccggag ttcctgttag
     1801 agaattgttg ctctcccggc gaattctatt atacactccc atcagctttg tcgaacctgc
     1861 tgaaatccca gtagacccgt tgggacctcc ggtcttttca ttcagggagg cgagacgctt
     1921 cccgacgggg gagtccggcg gcggtcctct gggcaatcat attttttgga atcaggcaaa
     1981 aaagtctcat ccaattcttc gaatctgccg aatgaagaag agatgaggta tgaagttcct
     2041 ttccgattga ttttccagct tttctcgatc ctccggctcc tggacgccga acggccttca
     2101 gggtttctgt gggatgtctt gtgcaggacg gactctaaag agagacaggc ccggcgtcgt
     2161 ccaggaacaa tcaggatagg tcttgcagga gtgtaagatg aaaaagcttc tctttgcctt
     2221 catcgtcatg atttgcctct tgtctccgat ccccagttca accctggccg acaggtgcag
     2281 cgctcccgcc ttcgttgccg cttccgcccc tcctctggtg atgttcgtca tggaacggga
     2341 ccatcggctc tattatgagg cttataacga cgcctcggac ctggacgagg acgggatgct
     2401 ggacgtggga tacaagcatt ccatcgacta tttcggctac tttgattcgt acaagtgcta
     2461 cacctacgtc tcaaccggca caaggaggtt cgtccccgcc cgggtcaccc caaacaagta
     2521 ctgcggcggg gcggaagaat ggagcggcaa tttcctgaac tggctttcca tgtcccgcat
     2581 ggacgttttg agaaaggtcc tgtacggagg gactcgggtc accgattcaa gctccgagac
     2641 ggtgctcgag ggggtctaca ttccccagga cgcccacagc tgggggaagg aatacgccgg
     2701 tgcggacacc cgggacctga ccccctggga cccccccacg tcccccaacc gccatctctt
     2761 ttgcatgacc agcaccagcg aaggttctcc ccacctcatc cgggtagccc gaaacgattc
     2821 ccaccgcatc tgggagtggg cgtccacgga aagggcggtc tgcggaggac ccgacacccc
     2881 ggccggttac cggcaagccc ccgtggccac tcgcggcgac attctggatt atatcgtgcg
     2941 ggtgcaggtc tgcaacggga gtgtcggcaa cgagcccaat tgcaaagaat acccgggagg
     3001 cgggggagtg agaaagccga ccggcctcct tcagatctat gccgaaggag acggcaccaa
     3061 ggtgtgctcc aagacgtgga aatcgtgcaa caccgattcc ggctgcggcg ccaccgaagg
     3121 cctctgcgtg gacaaaaccc agatgtacct ggggttgatc accgggtcct atgtaaagaa
     3181 tttgagtgga ggggtgctcc ggaagaacat ctggggagtc ctggacgaaa tcaatgcaca
     3241 gagcgggtcg ttccagagtt ccgaaaacac ccagggcaac atcatcctta ccctcgaccg
     3301 catgaagacg gtgggattcc ggtattccga ttactcttat caaggaacgg cgggaggcac
     3361 ctgtggctgg attacggatc ggcccattaa cgaaggggaa tgccgcatgt ggggaaaccc
     3421 catcggggaa atgatgtatg aggcccttcg ctattttacg gagaaaacca gccccaccgg
     3481 caacttcacc tacagcacca cccaggacgc aggcctcagc ctcagcaaac ccagctgggg
     3541 catgcccaag ggcagcagca ccctgaccct ctacaacatt tttccgtcct gcgccaaagg
     3601 gttcctgctg gttctcagcg acgtcaacaa cagctacgat tcggaccagg ttcccggcgc
     3661 atactccact ttcgccgcat ccaccttcac gggagatttt cccggactcg acgtgaccgc
     3721 ccttctgaac gacatcggga cccgggagtc cattcccggg ggaaactact ttgtcgggca
     3781 gagccaaagt gattttgatt ttatctgctc ctccaagtcg gtctcgaact tgagctccat
     3841 ccggggcctt tgtcccgagg aacccaccaa gcaggggcac ttttccccgg cggccatggc
     3901 ctattacggc aagtccttca tgaccataca aacgggcaag cccgacgtca ccaccctggc
     3961 cgtggccatg tcctcggcgg tccccgatat caaggtcaag gtgggagatc aatccgtccg
     4021 aattgctccg gtaggcaaat ccgtaagcgg cgattacaac gtttatacga aatgcgcggc
     4081 caactgcact cttgtccggg acgtcaacgg actccacatc agcaactgca atgccaacgc
     4141 ctattgtccc tccaaccaga tcgtggattt ttacgtcgag caaatcacct atgacgcgga
     4201 caagaacctg accaacgcca cgttccgagt caatttcgag gacgtggaac agggagcgga
     4261 ccatgacatg gacgccatcg tcatgtatca agtccagccc gtcggctcaa accaggtcca
     4321 ggttacggtc acatccagtt atgcagccgg gtccattgac caggtgctgg gcttcaccat
     4381 ctcgggaacc agcgaagacg gcctttatct ggtggtcagg gaccgggaca tgtgcggcag
     4441 cggaacggga acgtgcagcg acgccaacac tcccgccgcc gtgggcaacc ttccccttga
     4501 gtggaccaaa acgttcactg tggtgggaaa ccctgccggt ttgctcaaaa accccctctg
     4561 gtacgcggcc aagtacggcg gtttctccga ctccaacggg aatcgcattc ccgacctgac
     4621 ccctgagtgg gacaaggatg gagacggggt cccggacaat tatttcttcg tggacaaccc
     4681 gttgaaactc tacgaacaga tggagcgggc attcattgcc atcctgaacc ggggaggcag
     4741 cgccggggcc gttgccacgg tgacccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnntca ttgccatcct gaaccgggga ggcagcgccg gggccgttgc cacggtgacc
     4921 caacaggtgg aaggggagga catcgtcatg cggggcgcat tcacttccta cgagctggac
     4981 ccgaaccact tcgtctggaa gggacacctc gagtcctact ggccctatgg cggctgttcg
     5041 gactacctga cccaaagccg ttgtgaggaa ctggtggggt gcaaatggca aggttcgtcc
     5101 tgtaccggga gcctgtattc cttccaaaga gaggagcgcc tgggacaatt ctgcttcgag
     5161 caccacgacc actgttggac ggccgaggac cacctgccga cccccaacaa ccgaaccatc
     5221 tactcgatga tcaacggcag ccaggtggcc ttcaccagcg ccaacgtgtg cacggcgagc
     5281 gactggctga acctcgccgg ggattcgctt ttttccacga ccgattgcgc cgacctagtg
     5341 agttgggtcc gagggacgga cacctggacc aaggcccgtg accgggatga ctggatcctg
     5401 ggcgacattg tcttttcaac gcccgtggtg gtgcaatcac cctcccttgc cgccgtgccc
     5461 agtgccgccg tcggcgattg cgcccagacc gccgggcacc cgtgttcggg tggttgcgac
     5521 tgctcctcgg actgcgtcga gacctgctac tattgcttca gggaatgcaa caagacccgc
     5581 aagaaggtgg tctatgtcgg cggcaacgac gggatgctcc acgccgtggt ggcaggcata
     5641 tggtacagcg acccgaaccc caacgtcgac ggagacgggg acggggtaaa ggatgaaagc
     5701 cattggatct acgatcccaa cgccccgaat tccgaatgcg acgggcaggt ctgcacggga
     5761 aaagaccaga tcggcaaaga gctctgggct tacatcccca gcaatatcct gtcggagctg
     5821 aaagaactgg cccgtcccac ctatgggacc caggggggct gcaaacaccg ttttctggtg
     5881 gatttgtctc cccaggcctg ggaggtctac atcgatcccg acgggaacgg tcgcaggtgg
     5941 cgcaccgtgc ttctgggcgg agagagagac ggtggggaca tgctgtttgc catcgacgtc
     6001 accgacccgg atcagccgaa aatcctttgg gaatacccca tcttgaggaa tatggtccag
     6061 atcaccgcca gcggcagcga ttacctggcg acccttcctt tcctggacaa gacggtatac
     6121 gaccaggtca aaaaccttcc ggcttcctgg tccatcccct acgtcgggaa gttgaagatc
     6181 ccaaccaacg taagctttct ggcagccaat gcgatcccat cctggacggc gggaaccccg
     6241 gccttgaccc tcagcaaccg agggcccaac gaccttagcg ggtgggtggc catcatcggc
     6301 gacacttccc gggtgttcaa ccaggccgaa cttcccggga ccctcacggc agcccagaaa
     6361 gacgcggccc tcaaacccca cctgttggcc atcgacatcg aaacaggtgt gaatctcttt
     6421 cagtacttgt ggcccatgct ccacggggct ttgcccgcgc aatggcccaa gaccgtctcg
     6481 gggaccaatg tcgtcccaca ttccatgacc tcgcccgtgg tgttggacct ttgggacagc
     6541 acgggccacc tcaaccccga cggctacctg gaccatattt acctggggga tttgaacgga
     6601 aacttctacg ggatgaagtt caacctggac gaaggaacca ccctgggaat gagcatggat
     6661 gtctggaaga ccaagcccgt tgccgccacg gacgtgggca gcaacaatta tcgttcttcg
     6721 cgggaaccca tttccgtgca gccgtcaacc gccctggacc aggatcagaa tgtgaggatc
     6781 tatttcggaa cggggaagtt cgacaacctg gaagggacaa gcaacgacaa gacggacacc
     6841 gccaagatgt ccttttacaa cctggtggac agcagccaac gccccagcat cactgcggca
     6901 tcctcggtga caaagatcag cgacagcccg acccgcttca ccgcatcggg cggcattgcc
     6961 tcgggcttca aaatgtacaa cttcggcatc gacatcaacc tccactgcag ctctcccacc
     7021 tacaacgcct catgtacctg ggtgaagagc gacggcaccc aagattgctg cgaatcggcc
     7081 tgcgcttccc cggcgaatcc ttgctgggac tgcatctatg ggctgaattt ccccggtgaa
     7141 cgggtggtgg attcggcgct ggtagccgga ggcctggtct tcttcaccac cttcattccc
     7201 aaatcggaac cctgctccgc cgggggtgac agttacttct acgtcttgga ttaccagtgc
     7261 gggagtctca ccacaaaccc gttcggacaa tccaactttg tcctgagaag ctcggttcag
     7321 gacctcacca acccgggaga ttacgcggcc gtcaccgagg gaaccaagac cgtggcctat
     7381 gtggccaagc tgggacccgg aatgcccagc cggcccgtgc tcgattcctc cggggagtat
     7441 gtcttcatcc agacgagcga cgcccaaatc cacaagatca aagtggagct cctcttccag
     7501 cccttcgagc tcaggggctg gaaagaggaa ccccagtaat ggttgaaatt caagggtgag
     7561 tcgaaggggg cctcaacggg ccactttgag ggtgacggca cccaccgtgg attgggcatt
     7621 gcctttgccc gaaggaagag ggacggatcc ttgccccacc ggtcgatagt agaggccgtg
     7681 aaaggaggaa tatccctgcc agttgagcat ccagcccggg gggggcgtcg tcctgtaccc
     7741 caggtagtcg atccggggag taatggtccc ctgccccatg gactggg
//
LOCUS       DAOOVC010000173         7763 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5686_length_7763_cov_5.036066, whole genome shotgun sequence.
ACCESSION   DAOOVC010000173 DAOOVC010000000
VERSION     DAOOVC010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5686_length_7763_cov_5.036066"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(395..748)
                     /locus_tag="PLM79_15800"
     CDS             complement(395..748)
                     /locus_tag="PLM79_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87824.1"
                     /translation="MKIQAIFEDGILKPTRPLRLKHKLVTIEIPDEEVLDEEVFDGEV
                     IDEELMDEQLGAEQIDVYKKYNLPPEAKAKADEMLARLAAIAEEVLSIPEDQLPELTE
                     KQRERFEAFKLREDR"
     gene            complement(726..1361)
                     /locus_tag="PLM79_15805"
     CDS             complement(726..1361)
                     /locus_tag="PLM79_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87825.1"
                     /translation="MAILTHEGKYPCGFVLRLLDWERYNSARREPEVRNVVPLGEPYT
                     LWVKYRMALQVGDTTIYESQKDTTLVLEDIERLIQELDRLANGKTNAVGFDPIEPDFG
                     LAIRNLTESEASVSLSSAAEIRAGVPTGAVNESDDPAPLFDAQVWIDHPNQVDRFYGG
                     YGPGLYFQAEVADVERFTQELREELKGLGPYFKGNQTTSKNGSKNEDPSHL"
     gene            complement(1478..1810)
                     /locus_tag="PLM79_15810"
     CDS             complement(1478..1810)
                     /locus_tag="PLM79_15810"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV87826.1"
                     /translation="MTEKIDVIEGTMNVFTDLGLPDAAERQTKTRLAYVLNEIIKSRK
                     LKQVEVARLLNIPQPKVSALRNYRLDGFSVERLLGFLTALDQDIEILIRPRSDHMRSG
                     RISVLEVR"
     gene            complement(1803..2207)
                     /locus_tag="PLM79_15815"
     CDS             complement(1803..2207)
                     /locus_tag="PLM79_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOV87827.1"
                     /translation="MTRLPAAPREIPLHWVGSSKKDLLEFPDVVISNMGYALGLAQIG
                     GKHPRAKPWKGHGPGVFEIVDDYRGDTFRAVYTVRFPKAVYVLHAFQKKSKSGIKTPQ
                     SHIDLIGERLKRAAQDYEQRYGQSEPRGKGHD"
     gene            complement(2585..3121)
                     /locus_tag="PLM79_15820"
     CDS             complement(2585..3121)
                     /locus_tag="PLM79_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87828.1"
                     /translation="MSEGKKKLFLYEALELRGELDARIKTLKDCLPETRQNRGRLSLL
                     GGEEGIRRPSSDFDPSAARSALRKLEFKRRKLNSAIQQANFTQKVEYEGELISLNEAL
                     EARKALNERIGELHQQAVSSAYERVIYKEGRDIVEANEPPYSECVEKLEQARLSFRVL
                     NRLLRRASFETVVDFQDE"
     gene            3483..3734
                     /locus_tag="PLM79_15825"
     CDS             3483..3734
                     /locus_tag="PLM79_15825"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HOV87829.1"
                     /translation="MGRLCVDYPQSLPAVLNLSPESFEEEAKMALAVKLFEMGRLSSG
                     QAASLAGVSRVAFLLNCRRFGAASVEWDESELKAEFEEL"
     gene            3739..4233
                     /locus_tag="PLM79_15830"
     CDS             3739..4233
                     /locus_tag="PLM79_15830"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HOV87830.1"
                     /translation="MMPSIVCNTGPLIALAMIDRLDLLKLLFRKVLVPEAVHGEILQG
                     GSASTGLSAYRQASWIKVIRLRKLPDPLLDSNLDVREASVIQLARERRAKLVLIDERK
                     ARKVARNVYGLRLIGSARVLVEARRQGHLESVSEAIRRIREGGYWIHDDIVRLALHEA
                     GESP"
     gene            4285..4680
                     /locus_tag="PLM79_15835"
     CDS             4285..4680
                     /locus_tag="PLM79_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87831.1"
                     /translation="MSLKRSDLIKKLNEASSELLREKGYISFVDVLIKTGKLKQDGYE
                     DWRFGRIPYLEKAITVNLAKVNHMLRTLQENAKHGGLKPSKTAYMSWGKGPKRPLRFS
                     KSGDPNIEQAYATHFLKPKEVRTQSLQED"
     gene            4722..5567
                     /locus_tag="PLM79_15840"
     CDS             4722..5567
                     /locus_tag="PLM79_15840"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOV87832.1"
                     /translation="MVQHETIRSIVHDFLKNRDASASLFGGNISVLRENERGAALSMV
                     INGAKSSYRLITVVEAEEKGYLINFIVELLDPKSLAGEIKDKGAEFFSTMVVRLNEPL
                     PGSPFIFDPKHPSLLCSALADELLDEAVTEDLLKELYAAIHYLVRQYERYYDALLLEK
                     VGKPYPEGFKCKQCGHCCMNLDAHSMSVPQKDVDRWREAGRYDILEWVESIPIGNGEF
                     VHDIWLSPVTGEDVKRCPWLRKLPKKDKYICRIQDLKPTVCRVFPQSRKHAEECRCKG
                     FEDTT"
     gene            5977..7452
                     /gene="casA"
                     /locus_tag="PLM79_15845"
     CDS             5977..7452
                     /gene="casA"
                     /locus_tag="PLM79_15845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HOV87833.1"
                     /translation="MNLLTDRWVPIKDKENGDRPRHVGLRDILCGREPEAAIYLWRDD
                     LEFAALHLLVVLTQVIAAPADARALKEREALPMTEAEYDALTEPYIDWFDLTHPERPF
                     LQIRHEGKETAIQKLFPGLPDGNNHMFFHRPGEIGEICLSCASIVLWNQANNVPSFSG
                     GFKGSLRGGSPITVLLYHPELRRCIWRNVLSEEVIGAHFPQNGSELPVWVTGLPGRGS
                     VVESADIGFARGLLWQPACVELIVEEREGQCNSCGSPSNRLVREFKKQRWPYEVRGLW
                     PHPHGSIRTLQKKGKQGKELLSVFLSLNNTIPPWRQLLDILLEKEDPADGYRRALVLT
                     QYSEVWKGSSLPMAIGGYVSNQAAVVGRRHEVITLSAGWEKRLDELQKIVGLALDLKD
                     ILLNKMKYFFIKVLGTDLGNKAARKGAGSFFERTERLVLAALQNGPPPDLKERLERIA
                     WSVFNELSEPYRHSPKGLTAHAAAKSSLGYALKQLGNKKGE"
     gene            7458..>7763
                     /locus_tag="PLM79_15850"
     CDS             7458..>7763
                     /locus_tag="PLM79_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87834.1"
                     /translation="MAEKLMEESESGEKRTTPEQKREGLEGRHDFIAIRNRFDQLSSG
                     YRAAFRRAASPRDLVEVPAAYRLIPKEENLHGGWQRVLFLLPYIAHAENKRLGAALAV
                     "
BASE COUNT         1824 a         2101 c         2081 g         1757 t
ORIGIN      
        1 ggcggcttga gagccgcttt tcctaatgat ttcaatgttg gtgccgaagg ccggattcgg
       61 ccccgtgctt gaaatcatta ggaaaatcgg cactgcatac caatccgata gccatggaga
      121 gacactgagg gacatggcga gacattttgt tgccgagcag cgagtccatc acatggcctc
      181 aaagccagaa gagcacatat tcagcatgtt tcgaagggca catttttggc ctggttcttg
      241 gactttcttg tggtgcatcc actttttccc gcatggtcag caggaccctt tgaggctcgt
      301 cgagggaagg gaccgttacc atctgctact tggggcatag tcttcagtag gaagaggcga
      361 tccatctcgt tgacgtcgag caacagatcg ctcatcaacg gtcctcgcgc aatttgaatg
      421 cctcgaatcg ttcgcgctgt ttttcggtga gctccggtag ttggtcctcg gggatgctca
      481 ggacttcctc cgcaatggcg gcaagtcttg ctagcatctc gtcggccttc gccttggctt
      541 cggggggcag gttgtatttc ttgtacacat caatttgctc cgcgccgagc tgctcatcca
      601 tgagctcctc atcaatgacc tccccatcga aaacttcctc atcgaggacc tcctcatcgg
      661 gtatttctat ggtgaccagc ttgtgcttta agcgcaaagg tcgggtcggc ttgaggatgc
      721 cgtcttcaaa gatggcttgg atcttcattt ttgctcccgt tcttcgaggt ggtctgattt
      781 cctttgaagt acggccccaa ccctttaagt tcttcccgca attcctgggt aaatcgctca
      841 acgtcggcca cttccgcctg aaaatacagg cccgggccgt agcctccata gaaccggtcc
      901 acctggtttg gatggtcgat ccagacctgc gcatcgaaaa gcggggcggg atcgtcggat
      961 tcgttgactg ccccggtggg cactcccgct cgaatctccg ccgcggatga aagggaaacg
     1021 gaagcttcgc tttcggtcag gtttcttatg gccaggccga agtcgggctc gatggggtcg
     1081 aatccaaccg catttgtctt gccattcgcc agccgatcca gctcctggat cagtcgctcg
     1141 atgtcttcca acacaagggt tgtgtccttc tgactttcat agatcgtggt atcgccgacc
     1201 tgcaaggcca tgcggtactt cacccacaac gtataaggct ctcccaacgg taccacattc
     1261 cttacctccg gttcccgcct ggcggagtta tatcgctccc aatccagaag ccttagaacg
     1321 aaaccacacg gatacttgcc ttcgtgggtc agaatcgcca tgccaaaagc gccttccctg
     1381 tccgattccc ggcttgtaga ctcggccggt ggtacggggt cacacatttg agagagaaaa
     1441 aacttctatc gagtgtgtag ccccctcgtc aatcgaatca gcgcacctca aggactgaga
     1501 tccgtccaga tcgcatgtgg tcgctgcgag gacggatcag gatttcaata tcttgatcga
     1561 gcgccgtcag aaatccaagc aaccgctcaa cagaaaaccc atccagccga taattcctca
     1621 gggcggaaac ttttggttgg ggaatgttca acagccgagc gacctcaacc tgttttaact
     1681 tgcggctctt gatgatttca ttcagcacat aagccaggcg agtcttggtc tgacgttcgg
     1741 cggcatcggg aagacccaaa tcagtaaaaa cgttcattgt accctcaatg acgtcaattt
     1801 tttcagtcat ggccctttcc ccttggctca gattgcccat agcgctgctc atagtcttga
     1861 gcagcgcgtt tgagccgttc gccaatcaga tcgatgtgac tttgcggagt tttaatccca
     1921 ctcttcgatt tcttttgaaa agcatggagc acatacacag ctttcgggaa gcgtacggta
     1981 tagactgccc ggaatgtatc acctcgatag tcgtcaacaa tctcaaaaac tccggggcca
     2041 tggcctttcc agggctttgc ccgcggatgc ttgccgccga tctgcgcaag gccgagcgca
     2101 taacccatgt tgcttatcac cacatcgggg aattcaagca agtccttctt ggacgaacct
     2161 acccaatgca aggggatttc gcgcggagcc gccggcaatc tagtcatgat acaagaatat
     2221 ccctgtttgg gtatattttc aagagaactc actcctcacc ggggtcaggt cggtcaggtc
     2281 tacacatttg acagaagtct ttcttctgtc aaatgtgtag acctgaccca tatgattgcg
     2341 tgaaatccat cgatctaatg cggaaaaagg gtggagacgc ggagccattc agctcaggtg
     2401 ctcagctgct caacttgctc tgcggtcttt cgctgagggg atatggagct ttaccggacg
     2461 gttcaagtcg tcaagccccc aagtatttgg ctgtcacaga agccatgatc tgggctatgc
     2521 tttgtcgcca aagcattcgc acttgctccc ctgtctccgt aaccgtttga tttatcctgg
     2581 aacctcactc gtcctggaag tccaccacgg tctcgaagct tgcccgtctg agcagcctgt
     2641 tgagcacccg gaaagaaagc cttgcttgct ccagcttttc cacgcactca ctgtaaggag
     2701 gctcattcgc ctcaacaatg tcgcgtcctt ctttgtagat gactctctcg tacgcggacg
     2761 aaacagcctg ttgatgcaac tcaccgattc gttcgttgag cgcttttcgg gcctcgaggg
     2821 cctcgttgag gctgatgagc tccccttcgt attcgacctt ctgggtgaag ttcgcctgct
     2881 ggatggcgct gttcagctta cgccgtttga attcaagctt gcggagggcg ctccgagccg
     2941 ccgacggatc gaaatcagag cttggccgtc tgatgccctc ttctcctccg agcaaggaga
     3001 gcctgccgcg attctgccgt gtctccggca ggcaatcttt caaggtcttg attcgtgcat
     3061 ccagctcccc cctcaattcc agagcctcgt acaaaaaaag cttcttcttt ccctcactca
     3121 tggcgcatca cctcccatgc cgacaaagca gatccgaacc tcgcttgatt ctcgcacacc
     3181 accagcccaa agagcaagag ctcatccggc attgtgttgg tcatcgttgg tcatcgagta
     3241 gccgatcaaa acctttcaaa acaaaacctt tgcttccaat tgacacgtaa tgcaagctcc
     3301 gttaaggtga acggagtggc gaccgaatgc ctgagagttc gacgcattct tcaagaagga
     3361 attgaatctt gtatcgcaaa gtacgctcgg cgggcaccac aaacacaaga ggagaccggt
     3421 tcaccaggca cgatctgtgc gaatctgtga agtctgtgga gcgaagaaga aggagtacaa
     3481 aaatgggaag gctatgtgtt gattatccgc agtcgcttcc cgccgtcctc aatctcagtc
     3541 ctgagtcgtt tgaggaggaa gcaaagatgg ctctcgcggt gaaactgttc gagatgggtc
     3601 gtctctcgtc cggacaggcg gcgtctctgg ctggagtttc acgcgttgct tttctattga
     3661 actgccgccg atttggggct gcgtccgttg aatgggacga atccgaactt aaagccgaat
     3721 ttgaagagct ctagccaaat gatgccgagc atcgtctgca acaccggtcc acttattgcc
     3781 ctggcaatga ttgacagact ggaccttctc aagctgcttt tccgaaaggt cctggtccct
     3841 gaagcggtcc acggggaaat actgcaggga ggcagtgcat caacgggatt atccgcatac
     3901 cggcaggcat cctggataaa ggtcataaga ttgagaaaac ttcccgatcc gcttcttgac
     3961 tccaacctgg acgttcggga agcttcggtc attcaacttg cccgcgagcg gagagcaaag
     4021 cttgtgctca tcgatgagag gaaagcacgc aaagttgcac gaaacgttta tggtcttcgc
     4081 cttattggaa gtgctcgcgt gctggttgaa gccaggcggc aaggtcacct cgagagcgtg
     4141 agcgaggcga tccggaggat acgtgagggc ggttactgga ttcacgatga cattgttcgt
     4201 cttgctttgc atgaagccgg agaaagtcca tgaagcctct gatccctgtt ccgcgcaagg
     4261 aaaccgtgag ggttgctata atccatgagt ctcaagcgta gcgatctgat aaagaaattg
     4321 aatgaggcct cgtcggagct ccttcgcgag aagggataca tcagtttcgt cgatgttctc
     4381 atcaagacag ggaagcttaa gcaagacggc tatgaagact ggagattcgg ccgaatcccg
     4441 tatctggaga aagcaatcac ggtcaacctg gccaaagtga accacatgct tcgcactctt
     4501 caggaaaatg caaagcacgg cggtcttaag ccgagcaaaa cagcatacat gtcatggggc
     4561 aaaggaccca agaggcctct caggttcagc aagtctggag atccaaacat agagcaagct
     4621 tacgcgactc attttctcaa accgaaagag gtgcgtacgc aaagccttca ggaagactga
     4681 cagcgaaacc aatccttctg taggagatac tgggcagcgg tatggtccaa catgaaacga
     4741 ttcgatcaat agtccatgat ttccttaaga accgggatgc gagcgcgagc ctttttggcg
     4801 gtaacatcag cgtgcttcgg gaaaatgagc gcggggctgc gctgtcgatg gttataaatg
     4861 gcgctaaaag ctcatacagg cttatcactg ttgttgaggc ggaggaaaag ggctacctca
     4921 ttaatttcat tgtcgagctg ctggacccga agtcattggc cggggagatc aaggacaaag
     4981 gggcagaatt cttttccacc atggtcgtaa gactcaatga acccttgccc ggcagtccct
     5041 tcattttcga cccaaagcac ccgtctcttc tgtgctcggc actggcagac gagctgctcg
     5101 atgaggcggt cactgaagac ctcctcaaag agctctatgc cgcaatccac tacctcgtga
     5161 gacaatacga gagatactac gacgctcttc ttctcgagaa ggtcggcaaa ccctatcctg
     5221 aaggcttcaa atgcaagcag tgcggccact gctgcatgaa tctcgacgcc cactccatga
     5281 gtgtccccca gaaggatgtg gaccggtgga gagaggctgg aagatatgac atcctcgagt
     5341 gggtggagag tataccgatc ggcaatggcg agttcgtcca tgacatctgg ttgagccccg
     5401 tcactggtga ggatgtgaaa agatgccctt ggcttcggaa acttccgaag aaggacaagt
     5461 acatttgcag gattcaagat ctgaagccaa cggtttgcag ggtgttcccg cagtcgagaa
     5521 aacatgccga ggagtgtcga tgcaagggat ttgaagatac cacataaagc accccctaat
     5581 ctcttccccc ttcacgcctt ttgttcgacg caatgattcc ccccatcgca ttactctggt
     5641 agaattatga gcttgcagtc gtattttaag tttcaggttt ttaagtttta ggtgccggcc
     5701 tcccggcttc atccaggagc aaacaaccac tggagcgtga ggaggggtca ggtctacaca
     5761 tttgacagaa gtcttttctc tgtcaaatgt gtagacctga ccccgggacg tgggcttcga
     5821 cgccggcgtc cgataccgaa agcgagaaag cgacgtccca tcgctcctca cacctaaaac
     5881 ctaaaactta aaacctaaaa cccacggatt cagccccaga ttaatctgtg aaaatctgtg
     5941 aaatctgtgg ataaaaacca acaggagacg ccaagcgtga atctgcttac cgacaggtgg
     6001 gtcccgataa aagacaagga aaacggagac aggccccggc acgtggggct gagggatatt
     6061 ctatgcgggc gcgagccgga ggccgcgatc tacctgtgga gagacgatct ggagtttgca
     6121 gccttgcacc ttctggtcgt gctaacccag gtgattgccg cgccggcgga tgctcgggct
     6181 cttaaagaaa gagaagccct gcccatgacg gaggccgaat acgatgccct caccgagccc
     6241 tacatcgact ggttcgatct cacgcatccg gaaaggccct tccttcagat ccggcatgag
     6301 ggcaaggaaa cggcgatcca gaagctcttt ccgggcttgc cggacgggaa caaccacatg
     6361 tttttccaca ggccgggtga gataggagag atatgcctct cgtgcgcatc gatcgttcta
     6421 tggaaccagg caaacaatgt cccttccttt tccggagggt tcaagggaag ccttcgaggc
     6481 gggtcgccca ttacggtgct cctgtaccat ccagagcttc ggcggtgtat ctggaggaat
     6541 gtcctctcgg aagaagtcat cggggctcac tttccgcaaa acgggagcga gcttccggtg
     6601 tgggtgaccg gccttcccgg gagaggcagc gtcgtggaat ctgcggatat cggttttgcg
     6661 agaggactgc tctggcagcc ggcatgcgtc gagctgatcg ttgaggaaag agaggggcag
     6721 tgcaactcat gcgggtctcc gagcaacaga ctggtgcggg agttcaaaaa acagcgctgg
     6781 ccgtacgaag tgagaggctt gtggccgcat ccccacggaa gcatccgcac tcttcaaaag
     6841 aagggcaaac agggcaaaga gcttctttcg gtcttcctca gcctcaataa caccatccct
     6901 ccatggcgac agctcctgga tatcctcctg gaaaaagagg accccgcgga cggctaccgt
     6961 cgagccctgg tgctcaccca gtacagcgaa gtgtggaagg gatcttccct tcccatggct
     7021 atcgggggtt atgtctcgaa ccaggccgcc gtagtgggac ggcgccacga agtgatcacc
     7081 ctgagcgccg gctgggagaa acggctcgat gagctccaaa agattgtggg gcttgcgctc
     7141 gatctaaaag acatcctgct caacaaaatg aagtacttct ttataaaggt gctgggcacg
     7201 gatttgggaa acaaggccgc aagaaaggga gcgggatctt ttttcgagag aacggagcgg
     7261 ctcgtccttg ccgccctcca aaatgggccc cctcccgatc ttaaggagcg cctcgagcgc
     7321 atcgcctggt cggttttcaa cgaactgagc gagccctacc ggcacagccc caaaggcctt
     7381 accgctcacg cggccgcaaa atcatccctc ggatacgcac tgaagcagct cgggaacaaa
     7441 aagggagaat aaaaggaatg gcagaaaagc taatggagga gtcggagtca ggcgagaaga
     7501 gaacaacccc ggagcaaaaa agggagggat tggaaggaag gcatgatttc attgccatcc
     7561 ggaaccgatt cgatcagctc agctcaggat acagggccgc ctttcgccgt gccgcatcgc
     7621 cgagagatct tgtcgaggtt cccgcggcat atcgcttaat tcccaaggaa gaaaacctgc
     7681 acggcggctg gcagcgggtc ctcttcctcc tgccgtatat cgctcatgcc gaaaacaaac
     7741 gccttggcgc cgcgctcgcc gtg
//
LOCUS       DAOOVC010000174         7716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5730_length_7716_cov_6.588174, whole genome shotgun sequence.
ACCESSION   DAOOVC010000174 DAOOVC010000000
VERSION     DAOOVC010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7716
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5730_length_7716_cov_6.588174"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1522
                     /locus_tag="PLM79_15855"
     CDS             104..1522
                     /locus_tag="PLM79_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87835.1"
                     /translation="MVRAAAAEDVSQMIRDAERPETRFADVYGAASAKAELEYIVRWL
                     NDPKQYRQLGLKPPRGILLYGDPGTGKTMLARALAGESRAAFLVESASSFVTKWVGSG
                     PENVRNLFARARRYAPSIIFIDEIDAIGKKREGGPSSRPQEETLNALLTEMDGFGTST
                     TRPVVVLAATNLVEHLDEALKRRFDREIEVDKPDRGAREAFLRSRLRGSPHRQVSDAV
                     IHRMAGQSANMTIADLDRVIQAAGRAASRGDGIITDPIIEEAFETMRMGEARGRVDPG
                     ALLRVARHEAGHCLIGWLRGEKPVQVTIVARGRAGGYVEREADEDRTAYTRREIEGII
                     RQCMGGRAAEILYYGEEDGLSSGVSADLEQATAWAQKMVTSYGMGDEIGPVAVGLGRI
                     EDGPMAVRVAQEAKKIIGGQLDRAIEELTRHKTRLDTLVEGLLDRNRLTRSELEALLG
                     EGPEGTGGKAGKAGGSEAVWSG"
     gene            1809..2270
                     /locus_tag="PLM79_15860"
     CDS             1809..2270
                     /locus_tag="PLM79_15860"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOV87836.1"
                     /translation="MFSFGCGTENPKGLHMSFFRVGEQLESDIILDEHCVGWENIAHG
                     GIVSTILDETMGWAVIAFKRVFFVTRVMEVRYHRPTPIRVPLKAVARIHPKPALRGCR
                     VRGILYDRHGTRLASAQAEMAYLSEKMQALLPESYRREMVRVFREIEQLPP"
     gene            complement(2283..5780)
                     /locus_tag="PLM79_15865"
     CDS             complement(2283..5780)
                     /locus_tag="PLM79_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV87837.1"
                     /translation="MRFEKILTSWSIRTKLLLLIVAVFLPGAGVIIHDGLENRKKDLQ
                     AAKRDATLLVDTLAAQQEKIAMGTKQMLSTLARLPQVRNLDAEACSKLFRELIEHNPL
                     YYAVGASTPDGTMFANNRAFESGTVNIAGQKHVRDALETRDFSVGEYVIGKITRVPSL
                     HYAYPVLDENERLIAVVTAAFRLSEYDALIRKASLPPDSSVLILDHRGVRLYRYPESD
                     AAPLGRPPTIGFDEIGDRDEGTYERNSDDGIYQLFAFKRLRLTEDAPPYLLIAVGIPK
                     DKILQKANIAMLNSLLFLFTAAVVAACLAWFLGNLVLIRPLNHLVAATQEFGSGRMDA
                     RTGLPHTPDELGKLAQSFDNMAALLEQRTMQRKKSAQELIRLYRQNQLILNAAGEGII
                     GLDENGTITFANPAAREILGYDEEELIGRDLHRTIHRVSHDGTLQPLTECPVWLCLHK
                     GVSGRVRDQMLWRKDGTSFPAAYSSTPILEDGRVVGAVVTFRDRSAYKRAEEALQKSE
                     HFVRRVIETTPNLLYIYDLAEKRNIYMNRETRAFFGYGPEEVQTLGWFSSQSPFHPDD
                     LPVIEAHREALSKAGEGDVRGMEFRARHASGEWRWLTSHEVVFARSPEGAVTQILGSA
                     QDITQRKRMEEALGESVASYKALAENLPGIVYRSFPGGQGMLFFNEMLESITGYTSGE
                     LASRGIFGMEPLILEEDRQRVAAAVGNALKERKPFEVSYRLRHKNGSTKHCFEHGRPI
                     YDEGGEFLHVEGVIFDTTERLHLENHLRQAQKMEAIGTLAGGIAHDFNNILAAILIYT
                     QMVLREIPESNPAHADLQQVVQSSLRAKELVKQILAFSRQTEEEKKHPLIVGSIIKEA
                     IQLLRASLPSTIEIRQKIASREDAALIDPTQIHQVLMNLCTNAAHAMREHGGVLEIGL
                     TKVRLGAEEILSWPDLKPGQYLQLTVSDTGHGMHPDTLQQIFDPYFTTKEPGEGTGLG
                     LAVVHGIVKKHGGAVRVRSELGKGSTFDVLIPVIEGDFRESLEEDADMPGGNERILLV
                     DDEEALLLGKKRLFEKLGYGVVAAGNGREALEAFQSRPDHFDLVVTDCTMPHMTGFDL
                     AREIKGIRPDIPIVLSTGGRAADLLEKAREAGILEVATKPLDLADLARMVRRILDQVQ
                     VEEEDEADRPRMRGPDPGL"
     gene            6488..>7716
                     /locus_tag="PLM79_15870"
     CDS             6488..>7716
                     /locus_tag="PLM79_15870"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOV87838.1"
                     /translation="MNNRDRPTSTIPTLARPLLWILLLALFSIGIYLRFYDTWTTDFM
                     ERVESQRRQDLVQLVSVARSAVEPILSEVRRGTLSRDEALQRVRTLVRAMTYEDTYGK
                     NYMFMSAYDGTMLVQPFEPTKEMTNQWDLRDDHGVYIVRELVKAARNRPEGAFVSYHY
                     HLPGLHGTQEKLAYVVGLPEVEAYLGTGMYRQKAIAEQSEIVDRVKRGALWLVLAVLV
                     PLSAAFLFLARRNNLVLAEMETRRRAEGALQESEERYRQLVENANSIILQWKESGEIL
                     YLNDYGRKFFGYDLEEIRGKHVVGTIVPETEETGRDLRAMIADIAARPGSYEKNVNQN
                     MRRDGTKVWIAWTNRIVSDERGRVSEIFSVGNDITEIRRIQKQLEESEERFRTAVEML
                     PLGIAILNRDGVPDFLNR"
BASE COUNT         1473 a         2361 c         2419 g         1463 t
ORIGIN      
        1 gcggcggcgg ctcgaaccat tcgaaggttc cgaagccgga tgaggatctc ctccgggcct
       61 ctcccggcag gaggagatcc tcatccggct tcggaacctt cgaatggttc gagccgccgc
      121 cgctgaagac gtgtcccaga tgatccggga cgccgaaagg ccggaaaccc ggttcgccga
      181 cgtctacggt gcggcctcgg ccaaggcgga actcgaatac atcgtccggt ggctcaacga
      241 tccgaagcaa taccggcaac ttgggctaaa gcccccccgg ggaatccttc tctacgggga
      301 cccgggaacg gggaaaacca tgctggcccg agccctggca ggggaaagcc gcgcggcgtt
      361 cctcgtggaa tcggcttcct cctttgtgac caagtgggtg ggaagcggtc ccgaaaacgt
      421 tcggaacctc tttgcccggg cccggcgcta cgctccgtcc attattttca tcgacgagat
      481 cgacgccatc ggaaagaaac gggagggggg cccgtcgtcc cgtccccagg aggaaaccct
      541 caacgccctt ctgacggaaa tggacgggtt cggcacgtcc acgacccggc ccgtcgttgt
      601 cctggcggcc accaacctgg tcgagcacct ggacgaagcc ttgaagcgac ggttcgaccg
      661 ggagatcgag gtggacaagc cggaccgggg ggcgcgggag gcgtttctgc gaagccggct
      721 ccggggaagc cctcaccgac aggtgtccga tgccgtcatc catcgcatgg cgggccagtc
      781 ggccaacatg accatcgcgg acctggatcg cgtgatccag gcggcgggac gagccgcctc
      841 ccgaggcgac ggaatcatta cggatccaat catcgaggaa gccttcgaga ccatgcgcat
      901 gggggaagcc cgggggaggg tcgatcccgg ggcgctgctt cgagtggccc ggcacgaggc
      961 cgggcattgt ctcatcggat ggctccgggg agaaaagccc gtccaggtga ccatcgtggc
     1021 acgaggccgt gccgggggct acgtcgagcg ggaagccgac gaagaccgca cggcatatac
     1081 ccgacgggag atcgaaggga tcattcggca atgcatggga ggccgggccg cagagattct
     1141 ctactacggc gaggaagacg ggctcagctc gggggtttcg gcagacctcg agcaggccac
     1201 ggcgtgggcg caaaagatgg tgacgagcta cggcatggga gatgagatcg gtcccgtggc
     1261 cgtcgggctg ggaaggatcg aggacggtcc catggccgtc cgggtggccc aggaggccaa
     1321 gaagatcatt ggggggcagc tggatcgggc catcgaggag ctcacccggc acaagacccg
     1381 cctggatacc ctggtggaag gactcctgga ccgaaaccgc ctcacccgct ccgagctcga
     1441 ggctcttttg ggcgaagggc ccgaggggac gggcgggaag gcgggcaagg cgggaggctc
     1501 ggaagcggtg tggagtgggt agagcgaagc gaagcccact gagggtgtaa aactgctctt
     1561 tacccacatc tgggatcagg gggggatgga tcgccgcctc ggggcgaacc cggagggttc
     1621 gcaaccattc gccgggggtc gaggccccaa gacccatacc cccggaacag ctcccaagag
     1681 gaaatcgacc catggggacg cgtcccgtga acaagagtgg gattgcgccc cccatgcccg
     1741 gtaggggctg ccggtcgccc ctgcaccctc tgcacgcacg tcaaaagcca tacggagacc
     1801 tgacccctat gttcagtttc ggatgcggaa cggaaaaccc caaagggctc cacatgtcct
     1861 tcttccgggt gggggagcag ctcgagtcgg acatcatcct ggatgagcat tgcgtagggt
     1921 gggaaaacat cgcccatggg gggatcgtct cgaccatcct ggatgagacc atgggttggg
     1981 cggtgattgc cttcaagcgg gttttctttg tcacccgggt catggaggtg cgctaccacc
     2041 gcccgacccc tattcgagtc cctctcaagg ccgtggcccg catccacccg aaaccggccc
     2101 ttcgaggctg ccgggtccgg ggaatcctct acgaccgtca cgggaccaga ctcgcttcgg
     2161 cccaggcgga aatggcctac ctttccgaaa agatgcaggc ccttcttccc gaatcctacc
     2221 gccgggaaat ggtccgcgtc ttccgggaaa tcgagcaact gccgccttga ttcggacggg
     2281 ttttacaacc cagggtccgg cccgcgcatc cggggtcggt ccgcctcgtc ctcctcctcg
     2341 acctgaacct ggtccaggat tcttcgcacc atccgggcga ggtcggccaa atccagcggc
     2401 tttgtggcca cttccaagat acccgcctcc ctggcctttt ccaggaggtc cgccgcccgg
     2461 cctccggtgc tgagaacaat ggggatgtcg ggccggatgc ctttgatctc ccgggccagg
     2521 tcgaagcccg tcatgtgggg catggtgcag tcggtcacca ccaggtcgaa atggtccggc
     2581 cgggattgaa aggcctccag ggcttcccgg ccgttgcccg cggccaccac cccgtagccc
     2641 agcttttcga aaagccgctt ttttcccaac aacagggctt cttcatcatc caccaggaga
     2701 attcgctcgt ttcctccggg catgtcggcg tcctcttcga ggctttcccg gaaatctccc
     2761 tcgatcaccg ggatcaggac atcgaaggtg ctgcctttcc ccagttcact ccggacccgg
     2821 acggccccgc cgtgtttctt gacgatgccg tggaccaccg ccaggcccag gcccgttcct
     2881 tctccgggtt ccttggtggt gaaataggga tcgaaaatct gctggagggt gtccggatgc
     2941 atcccgtggc cggtgtcgct caccgtgagc tgcaggtatt gtcccggctt cagatccggc
     3001 cacgagagga tttcctcggc gccgagccgg accttggtca ggccgatctc gagcaccccg
     3061 ccgtgctccc gcatggcatg agcggcattg gtgcaaagat tcatgaggac ctggtggatc
     3121 tgagtcggat cgatgagggc cgcatcttcc ctgctcgcaa tcttttgccg gatttcaatg
     3181 gtggacggca gggaagcccg gagcaactgg atggcctcct tgatgatgga accgacaatg
     3241 agcgggtgct ttttctcttc ctccgtttgc ctgctgaagg ccaggatctg cttcaccagt
     3301 tccttggctc gaaggctcga ttgaaccacc tgctgcaggt ccgcatgggc cgggttcgat
     3361 tcgggaatct cccgcaacac catttgggtg tagatgagaa tggcggccag gatgttgttg
     3421 aagtcgtggg cgattccccc ggccagggtc ccgatggctt ccattttctg ggcctggcgc
     3481 aaatggtttt ccaggtgtag acgttcggtg gtgtcgaaga tcaccccctc gacgtgcagg
     3541 aattctcccc cttcgtcgta gatgggcctg ccgtgctcga agcagtgctt cgtggatccg
     3601 ttcttatgcc tcaaacggta ggaaacctcg aagggttttc gctccttgag ggcatttccc
     3661 acggcggccg cgacccgttg ccgatcctcc tcgagaatca gcggttccat gccaaagatg
     3721 ccccgggagg ccagttcccc ggacgtatac ccggtaatgg actcgagcat ttcgttgaag
     3781 aacagcatcc cttgcccacc gggaaaagat cgatacacaa tccccggaag gttttccgcc
     3841 agggccttgt aggacgccac gctttccccg agggcctctt ccatccgttt gcgctgggtg
     3901 atgtcctggg cggagccgag gatctgcgtc accgctcctt cgggacttcg cgcaaaaacc
     3961 acctcgtggc tggtaagcca gcgccattcg ccgcttgcat gccgggctcg gaattccatc
     4021 cccctcacat ctccctcgcc tgctttggac aaagcctcac gatgggcttc gatcacgggc
     4081 aggtcgtcgg gatggaaggg gctttgggaa gagaaccacc ccaacgtctg gacctcttcc
     4141 ggcccgtacc cgaaaaaggc ccgggtttcc cggttcatgt agatgtttcg tttctcggcc
     4201 aggtcgtaga tgtagagcag gtttggggtg gtctcgatga cccggcgcac gaaatgctcg
     4261 ctcttctgca gtgcttcctc ggcccgcttg taggccgacc ggtctcgaaa ggtgaccacg
     4321 gccccgacca cccggccgtc ttccaggatg ggggtgctcg aataggccgc cggaaaactc
     4381 gttccgtctt ttctccaaag catttggtcc cgcacccggc cgctcacgcc cttgtgaagg
     4441 cacagccata cgggacactc ggtaaggggc tgaagggtcc cgtcgtggga gacgcggtgg
     4501 atcgtccggt gcaagtcccg gccgatcaac tcctcctcgt cgtagcccag gatttcccgg
     4561 gctgccgggt tggcgaaggt gatggtcccg ttttcgtcca gaccgatgat cccttccccg
     4621 gcagcattta ggatgagttg attctgccga tagagccgga tgagttcttg ggccgatttc
     4681 tttctttgca tggttctctg ttccagcagg gccgccatgt tgtcgaagga ttgggccagt
     4741 ttgcccagtt cgtccggggt gtgaggcaga cccgtccgtg cgtccatccg cccgctgccg
     4801 aattcctggg tggcggccac caagtggttc aggggcctga tcaagacgag atttcccaga
     4861 aaccaggcca ggcacgccgc gacgaccgcc gcggtgaaca ggaacagcag actgttcagc
     4921 atggcaatgt tggctttctg gagaatcttg tccttgggaa tgcccacggc aatgagcagg
     4981 tagggagggg catcctccgt gaggcgcaat cgtttgaagg caaaaagctg gtagattccg
     5041 tcgtcggaat tcctctcgta ggtgccttcg tcccgatccc cgatctcatc gaacccgatg
     5101 gtgggaggcc ggcccaaggg ggctgcatcg ctctccggat aacgatacag gcgaacccct
     5161 cgatggtcca ggatcaggac ggaagagtcc ggcgggagac tggcctttcg aatcagggca
     5221 tcgtattcgg aaagccggaa ggcggcggtg acgacggcga tgagccgctc attttcatcc
     5281 aggaccggat aggcgtaatg aagggacgga acacgggtga tcttaccgat cacgtattct
     5341 cccaccgaaa aatcccgggt ctcaagggca tccctcacgt gcttctgccc tgctatgttg
     5401 acggtacccg actcgaacgc ccggttgttg gcaaacatgg tcccgtccgg agtcgaggct
     5461 cccacggcat agtagagggg gttgtgttcg atcagttcgc gaaacagctt gctgcaggcc
     5521 tcggcgtcca ggtttcgaac ctggggcaat cgcgccaggg tgctcagcat ctgctttgtc
     5581 cccatggcga tcttttcctg ctgggcggcc agggtgtcca ccaacagcgt ggcgtcccgt
     5641 tttgcggcct gcaggtcttt tttccgattc tccagcccgt cgtgaataat caccccggca
     5701 ccaggcagaa aaacggcgac gatcaagaga agcaatttgg tgcgtatgga ccaggacgtc
     5761 agaatcttct caaaccgcat ggcggcctcc cgttgggaaa acatggttgg tcaccccggg
     5821 cctacccgaa ggcttccggc attcggtcga ggcggccctg gggaccggag cctcctcggc
     5881 gttccatggc gaacgcaaac tcacgggttt gaagggtgac ctcattcttc cggaaacaca
     5941 gcgggctagc cggcgcgcag cgaagatgag aaagagagca caaggatggg ataccaatat
     6001 ttgacggcaa tgggacgcag cagaaatggc ttgttggagc ccccgactct acttgatgca
     6061 gcattgcacg gatgacttta caccaattcc tgccgaatca caacccttcc tttgagacgg
     6121 ggcgtggatc ggcacgcggg actgtcgggc accccccccg attccggact cggggccgct
     6181 tcttcgcccc ccccggggca gacccgcttg gccgggatga ccccgttgcg gcagtggtca
     6241 aatatggagg catctggtaa aatgacgtcc tgtccgttgt gcgaggtgga cgcgtccggt
     6301 cccgggcgaa tctttccggc aaccggattc actcacgtga gaaccgcgga ccttccatcc
     6361 cgaccccgcg tatcccgacg tgcttcggcc cgtgccctcg tggtcgtttt atccatggcg
     6421 attcacaggc acggcggatg cgaacgtctg gaacccagcc gctgggatca actggaagag
     6481 aatgtccatg aacaatcgag atcgcccaac gtctaccatc cccaccctgg ccaggccgct
     6541 tctctggatt ctccttctcg cccttttctc catcgggatc tatctccgat tctacgatac
     6601 ctggaccacc gattttatgg aacgggtgga gtctcagcgc cgacaagacc tggtccaact
     6661 ggtgtccgtg gcccgcagcg ccgtcgagcc catcctgtcg gaggtccgcc gggggaccct
     6721 ttcccgggac gaggccctcc agcgggttcg gaccctggtg cgagccatga cctatgagga
     6781 cacctatggg aaaaactaca tgttcatgag tgcctacgac ggcacgatgc tcgtccagcc
     6841 tttcgaaccc accaaggaaa tgaccaacca gtgggacctt cgggacgacc acggggtgta
     6901 cattgtcagg gaactggtga aagcggcccg gaatcggccc gaaggggctt tcgtgtctta
     6961 ccactaccac ctgccgggcc ttcacggcac ccaggaaaag ctcgcttacg tggtcggcct
     7021 gcccgaagtg gaagcctacc tgggcaccgg gatgtatcgc cagaaggcca tcgcggagca
     7081 gagtgaaatt gtggacaggg tcaagcgggg ggccctctgg ctggtattgg ccgtcctggt
     7141 ccccctgtcg gcggcctttc tttttctcgc ccgccgcaac aacctcgttc tggccgagat
     7201 ggaaacccgc cgcagggcgg aaggggcact gcaggaaagc gaggagaggt accggcaact
     7261 ggtggagaac gccaacagca ttatcctcca gtggaaggag agcggcgaga tcctttacct
     7321 caacgactac ggccggaaat tcttcggcta cgatctcgag gagatccggg gaaagcacgt
     7381 ggtgggcacc atcgtccccg aaaccgaaga gacggggcga gatctgcggg ccatgatcgc
     7441 cgacattgcg gcgagacccg ggagctacga gaagaacgtc aaccagaaca tgcgccggga
     7501 cggcacgaag gtatggatcg cctggaccaa ccggatcgta tccgacgaga ggggccgcgt
     7561 ctccgagatc ttcagcgtgg gcaacgacat cactgaaatc agacgaatcc agaagcagct
     7621 cgaagagagc gaagaacggt tcaggacggc ggtcgagatg ctccccctgg ggattgccat
     7681 cctgaaccgg gacggggtcc ccgatttttt gaaccg
//
LOCUS       DAOOVC010000175         7701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5742_length_7701_cov_6.084881, whole genome shotgun sequence.
ACCESSION   DAOOVC010000175 DAOOVC010000000
VERSION     DAOOVC010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5742_length_7701_cov_6.084881"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..973)
                     /locus_tag="PLM79_15875"
     CDS             complement(197..973)
                     /locus_tag="PLM79_15875"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HOV87839.1"
                     /translation="MRSPLPSFLTLGFMLCSLAACVPRLPVIERPPAPTADQWTAKLL
                     DRALHWQSYQAKLNIRADSSKGKFRFQTVVLAKLPDRFRLETVNPWGQTVALLLLRGD
                     KALLWVPSEKTLYTAQRPEVLVAHFLGIPVHLETLGYGLTGSIPPEMLGNLTSVPGHP
                     RWLQYPGTPEPGRSLTWKFGFPSYALEAVDVLEASWNYTITYEPSVDLEASKFPRKIR
                     FASPQWEMDVTVDQIAPAPPMDDSSFDPPIPSGLRVIRLS"
     gene            complement(970..2682)
                     /locus_tag="PLM79_15880"
     CDS             complement(970..2682)
                     /locus_tag="PLM79_15880"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87840.1"
                     /translation="MGPGNLLFYVSFLFLLLSGACAQMGTVTQSFKPPSTKPPPASLP
                     KADVYFDYLTAQHHLRNSEIDQAIEAYNRALKKEPRSSSLLTELSALYLRQGKIETAL
                     KLAEEAVTYDPSYETVHMLLGQLYAGMARYDQAIASYQRVVELNPSNEDAYLLLGTLY
                     AQVGRFSEAMGVFDQLRDMFPDNPMAVYYRARIFLDMKFYSQAEAIYLEVLALDPTFE
                     NALLDLAYIYEVTDRLQEAQKTYQRILAFNNSSVQALTRLGNLFMREGKLDEALLLFG
                     QLIKLNRKDPESRLKVGIIHLQQKNYEDAIRAFTVLLQEQPHYDQAVYYLGSTYEEKG
                     DLEQAILNFRLVSRNSLLWNTAQIRLALIFAKKKDFRSGMEVLQEAIQKQPDLPATYL
                     YLGMLHEEQKQYEEALKALDRGLEKNSKDVDLLFRKGVVLDKMDRKKEAVEVMRKVLA
                     IEPRNSNALNFVGYSYAEMGVNLVEAKHMIKTALEIEPEDGYIMDSMAWVHYKMGQYR
                     KALEYILEALKRVPQDPVILEHLGDIYLSLGKRNKAAEAYQKSLESQHSEPEKIRQKL
                     EKLR"
     gene            3095..4330
                     /gene="amrB"
                     /locus_tag="PLM79_15885"
     CDS             3095..4330
                     /gene="amrB"
                     /locus_tag="PLM79_15885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOV87841.1"
                     /translation="MTDVKEDYPKLRYGLEGFPVVHEGRRMVLVRDRLGFCDEPLLFA
                     PPMAELLMHMNGENSLRDLQTQYMRITGTLLFLEHLQSVVRELDRRLFLENEGFIRAS
                     AERVARFQQDPVRRMQHAGRSYQLDPEALRAQLNAYFDPKNGGPGLPQPGVSDRPVVG
                     AVAPHIDLQAGGVCFSHAHKAACEGVLPETWVILGTGHEPIENSMALTIKDFETPLGI
                     VACDREYCRELAARVSRDITAGEYAHHREHSVEFQAVFLAHTQPHARIVPILCSFSLE
                     DWEEEKVHIDEAARAMRDLARENERRVGFMASVDFAHVGPRYGDRSVPRAGDVRENLS
                     EDRALLEILERCDAGEFLNRIRRDGNRRRVCGLAPLYVLARVLEGWSEGKTLQQGHAV
                     VDSHNSFVTFAAMIFYGKG"
     gene            complement(4387..5340)
                     /gene="epmA"
                     /locus_tag="PLM79_15890"
     CDS             complement(4387..5340)
                     /gene="epmA"
                     /locus_tag="PLM79_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HOV87842.1"
                     /translation="MNDRTRLARKRPNLQLRSVVLEAVRSFFLEQGFLEVQTPLRAPA
                     PLPELHIDAVPADGWFLLPSPEPFMKRLLAAGYDKIFQISPAFRRNETGRLHHSEYTL
                     LEWYRSHEDYGALQEDCRRLLSHVCRRTGRSGGFTHQGRRLEPEANWERLTVKEAFAR
                     FAGWTPDSNPDPFLFSRDLVEKIEPRLGFPAPCILLDYPAQQAALARIKESDPSVAER
                     FELYWAGIELANGFSELTDAREQRRRFERVLEERAGQGALSYPMPERFLQSLEDLPPS
                     AGIALGMDRLVMILADARSLDEVVAFPPDDEDLSPASDLRC"
     gene            complement(5482..6057)
                     /gene="efp"
                     /locus_tag="PLM79_15895"
     CDS             complement(5482..6057)
                     /gene="efp"
                     /locus_tag="PLM79_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOV87843.1"
                     /translation="MGATLTAGDLRKGLKLEIDGEPYLVVDFEFSKPGKGQALYRCRL
                     RNMITGNQFDRTYRSGDKFNAADLEEQDMNYLYKQDRSYVFMNNSTYEQIELSEEQVG
                     DARNYLIENQTVSILLFQGRPIGIGLPNFVALKVIKADPWVKGDTAAGATKPVTVETG
                     YVLQVPLFIEEGETIKLDTRTGAYVERTKSG"
     gene            6244..7530
                     /gene="eno"
                     /locus_tag="PLM79_15900"
     CDS             6244..7530
                     /gene="eno"
                     /locus_tag="PLM79_15900"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOV87844.1"
                     /translation="MSEIIAVKAREILDSRGNPTVEAEVLLSSGYTGVAAVPSGASTG
                     SREALELRDGDSARYLGKGVLQAVKNVNEEIAPQVLGMDARNQGALDQFLISLDGTEN
                     KGRLGANAILSVSMATARAAADECELPLYRYLGGCAASLMPVPHMNVINGGAHADNNL
                     DIQEFMLVPAGAKTFGEALRMGAETFHHLKKVLKSKGLNTAVGDEGGFAPNLRSNEEA
                     MEVLMEAIRAAGYDPGKDVFIAIDAAASEFYENGVYRLKAEAKPQKSADELVDYYEQW
                     VSRYPIVSLEDGMAEGDWDGWKELTQRLGKRIQLVGDDIFVTNTSILARGISEGVANS
                     VLIKLNQIGTVTETMQAIHMAYRAGYTAVISHRSGETEDTTIADLAVGAGTGQIKTGS
                     LSRSERIAKYNRLLRIEEELGSAACYAGLGTFPCRG"
     gene            7626..7700
                     /locus_tag="PLM79_15905"
     tRNA            7626..7700
                     /locus_tag="PLM79_15905"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7658..7660,aa:Cys,seq:gca)
BASE COUNT         1542 a         2195 c         2431 g         1533 t
ORIGIN      
        1 gtacagagtg tcgaagccga gaatgcgaag ccatcgggcc agcttcccca gcatgacgtc
       61 tgcaaaaaag agcttcctgg gggatgcctc cccttcttcc ggcctggaat gcatggctct
      121 ttcccggggg gtcgtcggcc gtgggtctgc gcgggggtcc cggaaccggg tccgcagatt
      181 tccctcggcc cggccgtcaa ctcaggcgga tgactcgaag cccgctggga atgggaggat
      241 cgaaggagga atcgtccatg ggcggtgcag gggcaatctg atcgacagtg acgtccattt
      301 cccactgggg ggacgcgaag cggatcttcc gggggaactt ggaggcttcg agatcaaccg
      361 acggttcgta agtaatggta tagttccagg atgcctcaag gacatccacg gcctccaggg
      421 cataggaggg aaatccgaac ttccaggtga gcgagcggcc gggttccgga gtgcccgggt
      481 attggagcca acgggggtgt ccgggaacac tcgtgagatt tcccagcatt tccgggggaa
      541 tggagccggt gaggccgtat cccagggttt ccaggtgaac gggaattccc aggaaatgag
      601 ccacaaggac ctcgggacgc tgggccgtgt aaagcgtctt ctccgatggg acccagagga
      661 gggccttatc ccctcggaga aggagcaggg caacggtctg gccccagggg tttaccgtct
      721 ccaaccgaaa ccggtcggga agttttgcca gtaccaccgt ctgaaagcgg aattttccct
      781 tgctgctgtc ggcccggatg ttgagctttg cctggtagct ttgccaatgg agggcgcggt
      841 ccaaaagctt tgcggtccac tgatccgcag tgggggccgg ggggcgctcg atgaccggaa
      901 gccgaggaac gcacgccgcc aaggaacaca gcatgaaccc cagggtgaga aaagagggca
      961 ggggagatct catcggagct tttccagctt ctgtcggatc ttttcgggct cactgtgctg
     1021 ggattccagc gacttctgat aggcttccgc cgctttgttc ctcttcccca ggctcaggta
     1081 gatgtcgccc aggtgctcga gaatcacggg gtcctggggc actcgtttca aggcttccag
     1141 gatgtattcg agggcctttc tgtattgtcc catcttgtaa tggacccaag ccatgctgtc
     1201 catgatatag ccgtcctcgg gttcgatctc gagggccgtc ttgatcatgt gcttggcctc
     1261 caccaggttt acccccatct ccgcatagct gtacccgaca aaattcaggg cgttggaatt
     1321 ccgaggctcg atggcaagaa ccttgcgcat gacctcgacg gcttccttct tccgatccat
     1381 cttgtccaga acgacgcctt tgcgaaagag caggtcgacg tccttggagt ttttctcgag
     1441 cccgcgatcg agtgccttga gtgcctcctc gtactgtttc tgttcctcgt ggagcattcc
     1501 caggtagagg taggtggcgg gcaggtcggg ctgcttctgg atggcttcct gcaggacctc
     1561 catcccgctt cggaagtcct ttttcttggc aaaaatgagg gccagtcgga tctgggcggt
     1621 gttccacagc aggctgtttc gagagaccag gcggaaattc aggatcgcct gttccaggtc
     1681 ccccttttct tcataggtgc ttcccaggta gtacacggcc tggtcgtagt gaggctgctc
     1741 ctgcaggagg accgtaaagg cccgaatggc atcttcgtaa ttcttctgct gaaggtggat
     1801 gatgcccacc ttgaggcgac tctccgggtc ctttcgattg agcttgatga gctgtccgaa
     1861 cagcaggagc gcttcgtcga gctttccctc gcgcatgaag agattcccga gcctggtcag
     1921 ggcctggacg ctcgaattat tgaaggccag aatgcgctgg taggtcttct gggcctcctg
     1981 gagccggtcg gtgacctcgt agatgtaggc aaggtccagg agagcgttct cgaaggtcgg
     2041 gtccagggcc aggacctcca ggtagatggc ttccgcctgg ctgtaaaact tcatatcgag
     2101 aaaaatcctg gcccggtaat acacggccat gggattgtcg ggaaacatat ccctgagttg
     2161 gtcgaaaacc cccatggctt cggaaaaccg gcccacctgg gcatagagag ttcccaggag
     2221 caaataggcg tcctcattgg acggattcag ctccaccacc ctctggtaag aagcgatggc
     2281 ctggtcgtaa cgggccattc cggcgtagag ctgtcccagc agcatgtgca ccgtctcgta
     2341 ggacggatcg taggtcaccg cctcttctgc cagcttgagg gccgtttcga ttttgccctg
     2401 gcgtagatac agggcggaga gctccgtgag aagagagctg gatcgaggtt ccttcttcaa
     2461 ggcccggttg tacgcctcga tggcctggtc gatctcactg ttgcgaagat ggtgctgggc
     2521 cgtgagatag tcgaaataga cgtccgcctt gggaagggaa gcgggtgggg gttttgtgct
     2581 gggaggtttg aaagattggg tcacggttcc catctgggcg caggcgccgc tgagaagaag
     2641 aaacaaaaag gacacgtaga acaggaggtt tcccggaccc atgaacgtca ccgcctaagg
     2701 caagaaaata ctaatagaaa acaatagcct agaactgcga tcaactatca catttgtcga
     2761 gtggcgtcaa tgttttccct tgacggaaag ggtctttttc gggatcgttc aacatccgtt
     2821 tcgggactca agcttgaggg ggccggcaag aggacgaccc cggatgaagc gggaaggatc
     2881 ggaccatcaa agggttgccc aaaacctcct catctgagat aaagaaagga caggtggagc
     2941 ttgttccacc tctcgacggg agggtccggg ggaggctgca aagcctgccc ggggatcggt
     3001 ccccgccacc cgatgaagcg tcggtcgccc ctctccgggg atgcaccggg cggcgccccg
     3061 acgctcccag ccgtgaaccg ggaggacggt gaggatgacg gatgtgaaag aggattaccc
     3121 gaagctccga tacggcctgg aaggttttcc cgttgtccat gaaggtcgaa ggatggtcct
     3181 ggtgcgggat cgattggggt tctgcgacga acccctgctg tttgcgcctc ccatggcgga
     3241 actgctcatg cacatgaacg gggagaattc cctccgcgat cttcagaccc aatacatgcg
     3301 gatcacgggg accctgctct ttctggaaca tctccagtcg gtggtgcggg agctggatcg
     3361 tcgcctgttt ctcgaaaacg agggattcat ccgggcatcg gccgaaaggg tggcccgctt
     3421 tcagcaggat cccgttcgcc gaatgcagca tgccgggagg agctaccagc tggatcccga
     3481 ggctcttcgc gcccaattga acgcctattt cgacccgaag aacgggggac cgggacttcc
     3541 ccagcccggg gtttcggacc gcccggtggt gggagcggtg gcgccccata tcgatctgca
     3601 ggcgggggga gtgtgcttct cccacgctca caaggccgcg tgcgaagggg tcctgcccga
     3661 gacctgggtg atccttggca cgggtcatga acccatcgag aactcgatgg ccctcaccat
     3721 aaaggacttc gagacccccc tgggaatcgt tgcctgtgac cgggagtatt gcagggaact
     3781 cgccgcccgg gtttcccgag acatcaccgc aggcgaatac gctcaccacc gggaacactc
     3841 ggtggagttc caggcggtgt tccttgccca tacgcaaccc cacgcccgca tcgtgcccat
     3901 cctttgttcc ttctccctgg aagactggga agaagagaag gttcatatcg acgaggcggc
     3961 tcgagccatg agggacctgg cccgggaaaa cgaacgccgg gtggggttca tggccagcgt
     4021 ggattttgcc cacgtggggc cgcgatatgg ggaccgatcc gtcccccgcg ccggcgacgt
     4081 tcgcgaaaac ctgtccgaag atcgcgctct cctcgagatc cttgaacggt gcgacgcagg
     4141 ggaattcctg aacaggattc gacgggatgg aaaccgccgc cgagtgtgcg gcctggctcc
     4201 cctttatgtc ttggccaggg tattggaggg gtggagcgaa ggaaagactt tgcagcaggg
     4261 tcacgcggta gtggattccc acaactcttt cgtcactttc gcggccatga ttttctacgg
     4321 caagggttaa atccccggac ggcctgtggg cccatgggtc tcatgcgctg cttcgggagc
     4381 cggtcgtcag caccgaaggt ccgaagccgg ggagagatct tcatcgtcgg gggggaaagc
     4441 caccacttcg tccaggcttc gtgcgtcggc aaggatcatg acaagtcgat ccatccccag
     4501 ggcgatcccc gcactgggag gcaggtcctc caggctctgc agaaatctct cgggcattgg
     4561 gtagctcaag gctccctgac cggctcgttc ctccaagacc ctctcgaagc gcctccgctg
     4621 ctcccgcgca tcggtgagtt cactaaaacc gttggcaagc tcgatccccg cccagtacag
     4681 ctcgaaccgc tcggcgacgg aagggtcgga ctccttgata cgagccaggg ccgcctgttg
     4741 ggccgggtag tccagcagaa tacagggagc gggaaaaccc aggcgaggct cgatcttttc
     4801 caccaggtcc cgggagaaaa gaaagggatc ggggttcgaa tcgggcgtcc aacccgcaaa
     4861 ccgggcaaac gcttccttca ccgtgaggcg ttcccagttt gcctccggct cgagccgccg
     4921 cccctggtgg gtgaatcccc cggaccgacc cgtcctgcgg cagacatggg acaagaggcg
     4981 tcggcagtct tcctgaagag ctccgtaatc ctcgtgggaa cggtaccatt ccaggagagt
     5041 gtattcggaa tgatgcaatc ggcccgtctc gttccttcga aaagccgggg aaatctggaa
     5101 gatcttgtcg tacccggcgg ccaggaggcg tttcatgaag ggctcgggac tgggaaggag
     5161 gaaccagccg tcggcgggga cggcgtcaat atggagttcg ggcaacgggg ccggggcccg
     5221 gaggggagtc tggacctcca ggaacccttg ctcgaggaag aaggagcgaa cggcctcaag
     5281 gacgaccgac cgcaattgaa gatttgggcg ctttcttgcc aggcgggtgc gatcattcat
     5341 ggcgggcgga gtccgttgag ggcctcaccc tcttttcggg agcgaggggc cgcccccgga
     5401 ccccatcccc gggagtccgg gggggtccct gtgagggtgc gttcaatgtc gggtcatttc
     5461 cggggacggt ttaaacggac tctagccgga cttggtccgt tccacgtaag ccccggtgcg
     5521 ggtgtccagc ttgatggttt ctccctcttc gatgaaaaga gggacctgca aaacataacc
     5581 ggtctcgacg gtcacgggct tggtggcccc ggcagccgtg tcccctttga cccaggggtc
     5641 cgccttgatg accttcaagg ccacaaaatt gggaagcccg atcccgatgg gccttccctg
     5701 gaagagaagg atgctcaccg tttgattctc aatgagatag ttcctggcgt ctcccacctg
     5761 ctcttcggaa agctcgatct gctcgtaggt gctgttgttc atgaatacgt agctgcggtc
     5821 ctgcttgtac agatagttca tgtcctgctc ttccaggtcc gcggcgttga atttgtcgcc
     5881 cgagcggtag gtgcggtcga actggttccc ggtgatcatg ttgcggagtc gacagcggta
     5941 tagcgcttga cctttaccgg gcttggagaa ttcgaagtcg acgaccaggt agggctctcc
     6001 gtcgatttcc agcttcaagc cttttctcaa gtcccccgcg gtgagcgtgg ctcccatagt
     6061 ggtccctttc tttagcgttg attaacccaa ccgaattttc tattttaatg gcttatgcgg
     6121 caaccggtca agctactttc cgtctcaacg tcctcatgtc ctacctaggg cgggcggatg
     6181 cccggaacac gagtcccgac gccatcccaa acgggaatac cacaaagagg aggcaaaaga
     6241 accatgagtg aaatcattgc ggtcaaggcc cgagaaatcc tggattcgcg agggaacccc
     6301 acggtggaag ccgaggtcct tcttagcagc ggctacacgg gagttgcggc ggtgccttcg
     6361 ggggcctcga cggggtcccg ggaagcgctg gagctcaggg acggggattc cgcccgttac
     6421 ctgggaaaag gggtgctcca ggcggtgaaa aacgtgaacg aggaaattgc gccccaagtc
     6481 ctggggatgg atgcccggaa ccagggggcc ctggaccagt ttctcatttc cctggacggg
     6541 accgagaaca aggggcgcct gggtgccaac gccatcctgt ccgtgagcat ggccacggcc
     6601 cgggcggcgg cggatgaatg cgaactgccc ctctaccgct atctgggagg ctgtgccgcg
     6661 tccctcatgc ccgttcctca catgaacgtc atcaatggag gggcccacgc cgacaacaac
     6721 ctggacatcc aggagttcat gctggtgccg gcaggggcaa agacctttgg ggaagccctg
     6781 cgcatggggg ctgaaacctt ccaccacctc aagaaggtcc tcaagtccaa aggactcaat
     6841 accgccgtgg gggacgaggg aggattcgcc cccaatctac ggtccaacga agaggccatg
     6901 gaagtgctca tggaggccat ccgggccgca ggatacgacc cgggcaaaga cgtcttcatc
     6961 gccatcgatg cggcggccag cgaattctac gagaacgggg tctatcggct caaggccgag
     7021 gccaagcctc aaaagagcgc cgacgagctc gtggattact atgaacaatg ggtgtcccgt
     7081 tatcccatcg tgtccctgga agacggaatg gccgaggggg actgggacgg ctggaaagag
     7141 ctgacccagc gtctaggcaa gcgtattcag ttggtgggag acgacatctt cgtgaccaat
     7201 acgtccatcc tggcccgggg aatttccgag ggagtcgcca attccgtcct gatcaagcta
     7261 aaccagatcg gcaccgtgac cgaaaccatg caggccatcc atatggcgta tcgtgccggg
     7321 tacacggcgg tcatctctca ccggtcgggg gagaccgagg acacgaccat cgcggacctg
     7381 gccgtggggg ccggaaccgg ccagatcaag acggggtcgc tctcgagaag cgagcgcata
     7441 gccaagtaca atcggctcct gcgcatcgag gaagaactgg gaagcgctgc ttgctacgcg
     7501 gggctcggaa ctttcccttg ccgaggctag gacctcccgg gggaaaaggt cccgcccgcc
     7561 ccatcccggg atcttctcct tgacaccgaa cccgttttcc gataatccat aggcacgttt
     7621 gccgaggcgg catacccaag tggtaaggga gcggtctgca aaaccgttat tcaccagttc
     7681 gagtctggtt gccgcctcca g
//
LOCUS       DAOOVC010000176         7689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5753_length_7689_cov_5.817134, whole genome shotgun sequence.
ACCESSION   DAOOVC010000176 DAOOVC010000000
VERSION     DAOOVC010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5753_length_7689_cov_5.817134"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLM79_15910"
     CDS             complement(<1..424)
                     /locus_tag="PLM79_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HOV87845.1"
                     /translation="MSGSVKTLGSSVDILLAMALQEDLGHGDVTTEAIFDPEHQARAV
                     VFGREPFVLSGSYGFLRVFQLLDSSVRAEPLARDGDLVPAQVPVFRLQGPTRALLAGE
                     RTALNLLQRLCGIATLTRQMVEALQGTSCRLLDTRKTTP"
     gene            complement(466..3147)
                     /locus_tag="PLM79_15915"
     CDS             complement(466..3147)
                     /locus_tag="PLM79_15915"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOV87846.1"
                     /translation="MMTENLLPKAYTFQDVEPRWSTFWEQHNLWTADPESPNPPFSIV
                     IPPPNVTGQLHMGHALNNTLQDILCRYHRLKGFEVLWVPGTDHAGIATQNVVERQLTQ
                     EGLNRHILGREAFLDRVWDWKQRYGGIIINQLKRLGASCDWSRERFTMDEGLSRAVRL
                     VFVRLYQEGLIYRGKRMINWCPRCMTALANIEVEGEELDSHLYHIQYPAAPGKGGVVV
                     ATTRPETMLGDTAVAVHPEDPRYKALVGRKLILPLVERPIPVIGDPYVDREFGTGALK
                     VTPAHDFNDFEIGRKHGLELVQVIGEDGRMNEAAGVYAGLDRHACRKRILKDLEKGGY
                     LVKTESYRHRVGHCYRCRSIVEPMQSLQWFVKTRPLADQAIAAVQEGKTRIVPAKWEK
                     DYFIWLENLEDWCISRQIWWGHRIPAWYCRQCGETAVSLDDPSSCPSCQSTQLEQDPD
                     VLDTWFSSALWPFSTMGWPDRTPELQKFYPTSVLVTAFDILYFWVARMMMMGLHFMGE
                     VPFHTVYVHALVRDAQGQKMSKSKGNVIDPLVTMDQFGTDALRFTLTAFAVQGRDVKL
                     SEERIEGYKHFINKIWNAARLLLMNLEGTETPEKIPAAPYGTVHRWILSRAQRVIEQV
                     QGAVEGYLFNQYANVMYQFAWHEFCDWYLEIIKQDFYGEDRERKIVALTTAAEVFKII
                     LILLHPIMPFVTEEIWQKMPRVSGSLRQARIPSVEKDRIDPEAEEEMELVMEVINGIR
                     NIRGEMNVPPASRVEAVCLNDDSRLRMLVKAHERTICEMARLSRLQVGSAREVPKPKI
                     AAGTMAKNVEVYVLLKDILDFDSESSRLQKELTKLEKEYSFTLKKLSNEDFLQRAPSE
                     VIERERDKGSRLGEKLEKLRSHYERIRTIQASASGAE"
     gene            complement(3284..4021)
                     /locus_tag="PLM79_15920"
     CDS             complement(3284..4021)
                     /locus_tag="PLM79_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87847.1"
                     /translation="MKKQKRSLELEEQEASSSQDLGLVSEDLDEEEIVIELQDVVEYP
                     EDLDDEEGGAENLEVELLDAEPGLDFRELDTKGVEDEDEDLFEHDLLKEFALSEEPVA
                     EKPSPGPEREGPAVEEDFFEKIAREEAESSMEEPELPPGPAPVEAAAPGVDAKSGKAP
                     PEAPPDSSVVSVAAVAAVPVPEVASRELSSSPAENEEINAILDQFLNRIEERVGQMIG
                     EAVEARLPQIVRSLLREEIERIKSQIP"
     gene            complement(4302..5378)
                     /locus_tag="PLM79_15925"
     CDS             complement(4302..5378)
                     /locus_tag="PLM79_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOV87848.1"
                     /translation="MRKDDSGHNVSARPLRPLLEEREEKVLAPEAQRSRLSRGRMRQE
                     SECPLRTSYQRDRDRIVHSKAFRRLKHKTQVFLSPTGDHYRTRLTHTLEVSQIARTIG
                     RALALNEDLIEAVALGHDLGHTAFGHGGERVLNEIVPGGFYHNEQSLRVVDVLERNGR
                     GLNLTLEVREGILKHSKGRTNPILPEPEQRAGTLEGQVVRLADIVAYLNHDLDDAIRA
                     EILKARDIPVQVRDHIGSTHSGRIHTLVDDIIYSSLANGLREVTMSAEMIERVDQLRA
                     FLFERVYEVPQVLEEFGRVSKIIEDLYACFLENDALFEQEIGPLSDSPTRERQVCDYI
                     AGMTDRYALDLYKRLFLPRPWMKI"
     gene            5416..6612
                     /locus_tag="PLM79_15930"
     CDS             5416..6612
                     /locus_tag="PLM79_15930"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOV87849.1"
                     /translation="MKTTLYRYSLKEQLAPFFVCLLGLGLVLITGRLFQLTRLLFVST
                     LTVLDVLELLWYAFPKLLQYALPMAVLLGILLAFVRLNSDNELVALRATGISFRQFLP
                     PVLTLGLLCTLLSACNMFYITPHTNSSFENKLRFLGRTNLPVLLREGTFITSIPKIVF
                     YFRSVNTTDLTIEGIFVQDQRQEKIRPAIVAQRAQILYQRDMSHVTFRIHNGIITRIP
                     ESLKDAQAVSFKTYDLTLSMDELFGTAQDGSKDRDELTWKELLEVIGRMTPDRQVRYL
                     TEMHQRLALPFSCLALGLVGAPLGAVFRHRSRLIGISMGVGVYITYYVLLSAGKGLAE
                     NQLIPSSLAIWLPNGAALLLASYLWNRVDRGWVIQVDALWSSGLRIRDRLRSVFPTDG
                     RPSSRS"
     gene            6609..7688
                     /gene="lptG"
                     /locus_tag="PLM79_15935"
     CDS             6609..7688
                     /gene="lptG"
                     /locus_tag="PLM79_15935"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOV87850.1"
                     /translation="MKIIPRYILRVFFPIGALVLFAFGGLYLVIDFFEKLDDFIENQA
                     SVGDTFLYFFYKLPMILSQGVSMSVLLGVMITLGILSRNREIIAMKAAGVSALAIARP
                     LLLGALLISVAHFAMAETVARAGGFRAQQLWDQQVKKKQGSQSWAFENAWYRGQGVLY
                     QIGLYDHRNKVMLRPSLFFLDGQFRLTQRIDARRLWWNDGVWTAEDGLVLRFEGETTY
                     QERFSEMTLDIEEKPDDFTAMERTPEDLSWFDLVRYGEKIRQEGYNAVPYEVELQLRM
                     AIPFTTFILALLGIAVALHRGLHGGIAAGVGIAVAVAAVYLTVLQTGIALATAGILSP
                     PVGVWVGNVIFASLAGYVWISNPKQ"
BASE COUNT         1371 a         2283 c         2346 g         1689 t
ORIGIN      
        1 ggggtgtggt tttccgggta tccaggaggc ggcaggaggt cccttggagg gcctcaacca
       61 tttgccgggt gagggtggca atgccgcaaa gtctttgaag aaggttcagg gccgtgcgtt
      121 ctccggccag cagggcccgg gtcggcccct ggaggcgaaa gacgggaacc tgggccggga
      181 cgagatcccc gtcccgcgcc agcggttccg cccggaccga ggaatccagc aactggaaga
      241 cccgcaggaa cccgtaggag ccggacaaga caaagggctc gcgtccaaaa accaccgccc
      301 gggcttggtg ctccgggtca aaaatggcct ccgtcgtcac gtcgccgtgg cctaggtcct
      361 cctggagggc catggcaaga aggatatcga cggagcttcc aagggttttt actgaacctg
      421 acatggggcc ggtcggcatt gcttcctact cccgcaggag gcgcctcatt cggcaccgga
      481 ggcgctggcc tggatggtac ggatcctttc ataatgggac ctgagttttt ccagtttctc
      541 tcccaaacgg cttcccttat cccgttccct ttcgatgact tcgctgggcg ccctctggag
      601 gaaatcctcg ttggacagct tcttgagagt gaaggagtat tccttttcga gcttggtcag
      661 ttctttttgc aggcggctgg attcgctgtc gaagtccagg atgtccttga ggagcacgta
      721 gacctcgacg ttcttggcca tggtgcctgc ggcgatcttc ggctttggga cttcccgggc
      781 gctccccacc tggaggcggg agaggcgtgc catttcgcag atggtccgct catgggcctt
      841 aaccagcatg cgaaggcggg agtcgtcatt gaggcagacg gcctccaccc gggatgcggg
      901 gggaacgttc atttcccccc ggatgttccg gatcccgttg atcacctcca tcaccagttc
      961 catttcctcc tcggcctccg gatcgatccg gtccttctcc acggacggga tgcgagcctg
     1021 gcgaaggctc cccgaaaccc ggggcatctt ctgccagatt tcctcggtca caaaaggcat
     1081 gatgggatga aggaggatca ggatgatctt gaacacctcg gcggccgtgg tgagggccac
     1141 aatctttcgc tcgcggtctt ccccgtagaa atcctgcttg atgatttcca ggtaccagtc
     1201 gcagaattca tgccaggcga attgatacat gacgttcgcg tactggttga agaggtagcc
     1261 ctcgacggcc ccctggacct gctcgatcac ccgttgggcc cggctgagga tccatcggtg
     1321 gacggtcccg tagggagcgg cggggatctt ctccggggtt tccgttcctt ccaggttcat
     1381 gaggagcaat cgagccgcat tccagatctt gttgatgaag tgcttgtatc cctcgatcct
     1441 ttcttcggaa agcttgacat ctcttccctg gacggcgaag gccgtgaggg taaaccgaag
     1501 ggcatcggtt ccgaactggt ccatggtgac cagggggtcg atgacgtttc ccttggattt
     1561 gctcatcttt tgaccctggg catcccgaac gagggcgtgg acgtagaccg tgtggaaggg
     1621 gacttctccc atgaagtgga ggcccatcat catcatgcgg gccacccaga aatacaggat
     1681 atcgaaggca gtcaccagga ccgaggtggg gtagaacttc tgcagctccg gggtccggtc
     1741 gggccacccc atggtggaga aaggccagag ggccgagctg aaccaggtat cgaggacatc
     1801 cgggtcctgc tcgagctgcg tgctctgaca ggatgggcag gaggaaggat cgtccaggga
     1861 aacggcagtc tcgccgcatt gcctgcagta ccacgcgggg attcgatgtc cccaccagat
     1921 ctggcgactg atgcaccagt cttccaggtt ttcgagccag atgaaatagt ccttttccca
     1981 cttggcgggg acgatgcggg tcttgccttc ctggacggcg gcgatggctt gatccgccag
     2041 gggccgggtt ttcacgaacc actgcaggga ctgcatgggt tccacgatgc tcctgcatcg
     2101 atagcagtga cccacccgat gccgatagga ttccgtcttg accaggtacc cgcctttctc
     2161 caagtccttg aggatccttt tgcggcacgc gtgccggtcc aggcccgcgt agactccggc
     2221 cgcttcgttc atgcgtccgt cttctccgat gacctgcacc agctccagcc cgtgctttcg
     2281 accgatttcg aagtcgttga aatcgtgggc gggggtcact ttgagggcac cggtcccgaa
     2341 ttcccggtcc acgtagggat ctccgatcac ggggatcggt cgttccacca ggggcagaat
     2401 cagttttcgg cccacgagag ctttgtaacg ggggtcctcc ggatgaacgg ccacggccgt
     2461 atccccgagc atggtttccg ggcgggtcgt ggccacgacc acgccgccct tgccgggagc
     2521 ggccgggtat tggatgtggt agagatgact gtcgagctct tccccctcga cttcaatgtt
     2581 ggccagggcc gtcatacatc gggggcacca gttgatcatc cttttgccgc gatagatgag
     2641 gccctcctga tacagccgga cgaagaccaa gcgcacggcc cgggacaggc cctcgtccat
     2701 ggtgaagcgt tcccgggacc agtcgcagga tgctcccagg cgtttgagtt ggttgatgat
     2761 gatgccgccg tatctttgct tccagtccca cacccgatcc agaaaggctt cccggcccag
     2821 tatgtggcgg ttgagaccct cctgggtcaa ttggcgctct accacgttct gggtggcaat
     2881 tccggcgtgg tccgtcccgg ggacccacag gacttcgaag cctttgaggc gatggtatcg
     2941 gcacaagatg tcctggaggg tattgttcaa tgcatgaccc atatgaagct gccctgtcac
     3001 gttggggggc gggatgacga tgctgaaggg cgggttgggg ctttcagggt ccgccgtcca
     3061 gaggttgtgt tgctcccaga aggtggacca gcgtggttcc acatcctgga acgtgtaggc
     3121 cttgggtaac aagttttcgg tcatcatacc gctcccttgc ggtgctcctt tcggtggtcc
     3181 ggcgggacac gagggcgccg gcttcgaggc ccctgccggg gggattgcgc tgccgggccg
     3241 agaccgccct gccctgccgc cgccggcagg ggcgctcaaa actctagggg atttgactct
     3301 tgatgcgctc gatttcttcc cggagcaggc ttcgaacgat ctggggcaac cgggcctcca
     3361 ccgcctctcc gatcatttgc cccacgcgct cctcgatgcg gttcaggaac tgatccagga
     3421 tcgcgttgat ctcctcattt tccgccgggg aagatgagag ctccctcgat gcgacttccg
     3481 ggacgggcac ggccgcgacg gccgccacag aaaccacgga agaatccggc ggcgcttccg
     3541 ggggtgcctt tccggatttt gcgtcgaccc cgggtgccgc cgcctccacc ggggcgggac
     3601 ccggggggag ttcgggttct tccatggaag attccgcttc ctctcgagca atcttttcaa
     3661 agaaatcctc ctccacggcc ggtccttctc gttccggacc aggggagggc ttctcggcaa
     3721 cgggttcctc cgaaagggcg aattctttca acaggtcgtg ttcgaaaagg tcttcgtcct
     3781 cgtcttcgac ccccttcgtg tccagttccc ggaagtccaa tcccggttcg gcgtcgagca
     3841 gttcgacttc gagattctcg gcgccgccct cctcatcgtc caggtcttcc gggtactcca
     3901 ccacgtcttg cagctcgatg acgatctcct cctcatcgag atcttcggaa accaggccga
     3961 ggtcctggga agaggatgcc tcctgttctt ccagttccaa cgaacgcttt tgcttcttca
     4021 tggcattctt tcgtctccga gtccgggacc gggggtcccg gaatgggtga atggccggga
     4081 gttccagcta ttggaattca ttcccaaaga tggcctgaga tccctcgaat ccttcgtttt
     4141 cattcaagat cccgggaaag gcatgccacc gataccacgg gggcaatggt ccgtcaagcc
     4201 gcatatgagg ggcaggaggc ggcggaggca tctgaggatg tgggtccccg gggaaggaga
     4261 tcgtccggga ccccatgtgg agcgggagct tcaggcctgc ttcagatctt catccacggg
     4321 cgggggagga agagtctttt gtagagatcg agagcataac ggtcggtcat cccggcgatg
     4381 tagtcgcata cctgccgttc tcgggtcggg gaatcggaca ggggaccgat ctcctgctca
     4441 aaaagggcat cgttttcgag aaagcatgcg tagaggtctt cgatgatttt gctgaccctg
     4501 ccaaattcct cgagcacctg gggcacctcg tacacccgct cgaacaggaa agcgcgaagc
     4561 tgatccaccc tctcgatcat ctccgcgctc atggtcacct cccgaagacc gttggcaagg
     4621 ctcgaataga tgatgtcgtc caccagggtg tggattcttc cggaatgagt gctgccgatg
     4681 tggtcccgga cctgcacggg aatatcccgg gccttcagga tctctgcgcg aatggcgtcg
     4741 tccaggtcat ggtttaggta ggcaacgatg tccgcaaggc gcaccacctg cccttccagg
     4801 gtgccggccc gctgctcggg ttccgggaga atggggttgg ttcggccctt ggaatgcttc
     4861 aggatgccct cgcggacttc gagggtgagg ttcagtcccc ggccgtttct ttccagcaca
     4921 tccaccacgc gcaggctctg ttcgttgtgg tagaatccac cgggaacaat ttcgttcaaa
     4981 acccgttctc ctccgtgtcc gaaggccgta tgccccaggt catggcccag ggctacggct
     5041 tcgatgagat cctcgttgag ggccagggcc cggccgatgg tgcgggcgat ctgggacacc
     5101 tcgagggtgt gggtgagcct tgtgcgatag tgatctcccg taggggagag gaacacctga
     5161 gtcttgtgct tcaggcgtcg aaaggccttg ctgtggacga ttcggtcccg gtccctctga
     5221 taggaagttc gcaggggaca ctcggactct tggcgcatgc ggccgcgact gagcctgctt
     5281 cgctgggcct cgggcgccag gaccttctct tcgcgctctt ccaacaaggg gcgcaagggt
     5341 cgtgcggata cgttgtgtcc ggagtcatcc tttctcattg accaaatccg tttcgattct
     5401 gtagagtgcc gctgtatgaa aacaaccctg taccgatact ctctgaagga gcaattagct
     5461 cctttcttcg tttgccttct gggtctgggc ctggtcctta ttacaggccg cctctttcag
     5521 ctcacccgcc tgttgtttgt gtccaccctc acggtgctgg atgttctcga actgctctgg
     5581 tacgcatttc cgaaactgct ccagtacgcg ctgcccatgg ccgtgctgct gggaatcctt
     5641 ctggccttcg tgaggctcaa cagcgacaat gaactggtgg ccctccgggc aaccgggatc
     5701 agcttccggc agtttctgcc tcccgtactg accctgggtc tgctgtgcac cctattgtcc
     5761 gcgtgcaaca tgttttatat cacaccccac acgaactctt ccttcgaaaa caagctgcgg
     5821 tttctgggcc gcaccaatct tccggtcctg cttcgagaag ggaccttcat cacgagcatt
     5881 ccaaagatcg tgttttattt tcgcagcgtc aacacgaccg atctcaccat cgaggggatt
     5941 ttcgtccagg accagcgaca ggaaaagatc cggcccgcca ttgtggccca gagggcacag
     6001 atcctgtacc agcgagacat gagccacgtc acgtttcgca tccataacgg aatcatcacg
     6061 cggattcccg agagcctcaa ggatgcccag gccgtgagct tcaagaccta cgacctcacg
     6121 ttgagcatgg acgagctctt tgggaccgcc caagacggat ccaaggatcg agacgagctc
     6181 acgtggaagg agctcctgga ggtcattggc cgcatgactc ccgatcggca ggttcgctac
     6241 ctgacggaaa tgcaccaacg cctggccctc cccttcagtt gtcttgccct gggtctggtg
     6301 ggcgctcccc tgggggccgt cttccgccac cggagccgtc tgatcggcat ttccatggga
     6361 gtcggggtct acataaccta ttatgttctc ctttcggccg ggaaggggct ggcggaaaat
     6421 cagctcattc ccagcagcct cgccatctgg ctccccaatg gggcggcctt gctcctggcc
     6481 tcgtacttgt ggaaccgggt ggaccgagga tgggtgatcc aggtggacgc tttgtggagc
     6541 agcgggcttc ggattcggga cagactccga tccgtctttc ccaccgacgg gaggccctct
     6601 tcccgctcat gaaaatcatt ccccgctaca tacttcgagt cttctttccc atcggggccc
     6661 tggtgctctt cgccttcgga ggtctctacc tggtcatcga cttctttgag aagttggacg
     6721 acttcattga aaaccaggcg tccgtggggg acacctttct gtattttttc tacaagctgc
     6781 ccatgatcct gagccaggga gtctccatgt cggtgttgct gggggtgatg attaccctgg
     6841 gaatcctcag tcgaaaccgt gaaatcattg cgatgaaggc tgccggagtc agcgctctcg
     6901 ccattgccag gcccctgctc ctgggggcac tgctcatctc cgtcgctcat ttcgccatgg
     6961 cggaaaccgt ggcccgggcc ggagggtttc gggcccagca actctgggac cagcaggtga
     7021 aaaagaaaca gggcagccag tcgtgggctt tcgaaaacgc ctggtaccga ggccaggggg
     7081 tgctgtacca gatcgggctt tacgaccatc ggaacaaggt gatgctcagg ccgtccctct
     7141 tctttctgga cgggcagttt cggctcaccc aacgcatcga tgcccggaga ctttggtgga
     7201 acgacggagt gtggacggcc gaagacggcc tggtcctgcg gttcgaggga gagaccacct
     7261 accaggagag attctcggaa atgacgctgg acatcgagga gaaacccgac gactttacag
     7321 ccatggaaag gactcccgag gatctgagct ggttcgacct ggttcgttac ggggaaaaga
     7381 tccgtcagga aggctacaat gccgtcccct atgaagtgga gctgcagctg cggatggcca
     7441 tccccttcac cacgttcatt ctggccctgt tggggatcgc cgtcgccctc caccggggat
     7501 tgcacggggg cattgccgcc ggggtcggga ttgcggtcgc ggtggccgcc gtttacctta
     7561 cggtcctcca gacggggatc gctctggcga cggcgggaat cctctctccg ccggtggggg
     7621 tttgggtggg caacgtgatc tttgcttccc tggccggtta cgtgtggatc agcaacccca
     7681 agcagtgag
//
LOCUS       DAOOVC010000177         7661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5780_length_7661_cov_3.306206, whole genome shotgun sequence.
ACCESSION   DAOOVC010000177 DAOOVC010000000
VERSION     DAOOVC010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_7661_cov_3.306206"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4178)
                     /locus_tag="PLM79_15940"
     CDS             complement(<1..4178)
                     /locus_tag="PLM79_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87851.1"
                     /translation="MIVSPPEYFIRISKEASRRWDQLESDPVLAGPWHQLFRQVQSPR
                     HVLSELLQNADDAGATWVRASLKDGVFEFAHNGNDFDEVTFQSLCQFGLSNKRHLHTI
                     GFRGVGFRSVFAFGPRVEARTPTITFAFEKKRFTEPRWINVPATEGETIFRVQLDGPS
                     KSAVLETEFKRWLKSPIPLLFFQSIVSLEIQGRVVHKEIVGPGPVSFSERIRLLNEQE
                     SELLCFRSAPEQFPRECLEEIREERGSQELDLPPCAVQIILGTPDPRLYTVLPTDVQP
                     LMPFSLNAPFVQDPARKEIKHPASSPTNAWLLERIGQLAGEAILGWLRNDRMSLGERA
                     RAYELLPKSVESDGSLNKETSLIILKGLRRAITSGQKVLLCHDGTLAEKGEAIDLPAA
                     VHDTWGTEKVLQVFGQGKKKAIAREIPARFIETLETWALVQRLGVTDIARRLILMNPG
                     PPCPEPLENLIFLWNFLQPLWNNWNFWNELQSLKIVPASQRSDLMPSGKLIAVGGKES
                     RISEDDWSFLMSWADIVNPKWVKLMTGTPLDSDHALASDLFNKLKLHERVGIEQVVKF
                     VAERIFTQNPGEAAIRLAHVAARGGVRVPDSFKFLCRDGRWRACSEHLIVENSEDLSQ
                     ILPESLFDSLVVSDAYESGLDQEYLGAWRDWASDVTKSKLSCFPMPVLTRTELYGKEK
                     LSNICVKRGGCAPSSYPIQSRWFELRDYDWKEALWEHWNQKAEDDPSFWKSIGISVVR
                     GWSEKWEETLDASIKQLGHTKAHSLGHGDLEVGWLLRLKSLPCLPDTFGRPAIPAELL
                     RTTSETMALMNIERFVHPDLDHPEYSKVLDLLGVRCQPSGFGTLLDRIRAISRSAVVP
                     VTPVIDLFRALDRATLRISHEELTELRHIFSEEALVFSDGCTWEKLSFIFQSNPENIP
                     GISTIHGELKDLSLWQRLDMAAQPTLETALQWLNEKKSNEVLNSSDREKVVQIVQRAP
                     AIVWQKCGAWLNLSGRWISKEDLRWGAVGHLSGLSLFESFRKKTADLTMLGDVAHDLC
                     REMGLEALDQALEMRLVKAAVTGYTPKPPWIETLGGILSRLRCPDIGSDANTQKCTYD
                     EDRKAAARLSRIGWQSVSELKLAPYMDGQPAGPEKSCKALWHDDTIYAQGSSPFHHRD
                     LVIEISRFFRTVVAKRAVQDCTDREPAWIIAYANEHLELDEEMERAPEPHDKIDDVIR
                     LRTDMEEVQTSEVSFVSTGEEIQTQHSSMVPPPGTAADSPAIEEVDPAQSGVEPEPIA
                     GHGDRKQRQEHLRDVFHNWIENQGFKYQDVTKSFISPDLQIIRRAEPPFQWIRYDREG
                     SELVRYWLGRGSLKKGVELPSEIWNWPLREGLQVLLVIWDSAEQLETYSLAELKRGAE
                     QGLVSLYTAKLMVRA"
     gene            complement(4175..7423)
                     /locus_tag="PLM79_15945"
     CDS             complement(4175..7423)
                     /locus_tag="PLM79_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV87852.1"
                     /translation="MLTRQPLRFLLADDPGAGKTIMAGLFIKELAARGDLQRCLICCP
                     GNLADQWQDELFGRFLLPFEILTKDRLETARTGNAFQEMNLLILRLDQLSRNEELQGK
                     LMQTDWDLIVCDEAHKMSASFFGGEVKETKRHKLGKLLSSITRHFLLMTATPHNGKEE
                     DFQLFMALLDGDRFEGRFRDGVHTVDVSDLMRRMVKEQLYKFDGTPLFPERLAYTVSY
                     KLSDLEAALYERVTQYVREEFNRADALNDDKRKGNVGFALTILQRRLASSPEAIYQSL
                     RRRRERLERRLREEELLQRGAEARLSMGADLPAVTEDDLEDLEDSPNAEIEEIEEKFV
                     DQATAARTIAELKAEIGTLKELEALALQVRQSGKDRKWEELSGLLQNNPEMFDTNGLR
                     RKLLIFTEHRDTLNYLTDRIRTLLGKPEAVITIHGAMGREDRKKAEEAFKQDKSTLIL
                     VATDAAGEGINLQRAHLMVNYDLPWNPNRIEQRFGRIHRIGQTEVCHLWNLVAEETRE
                     GDVYLTLLRKLESERIALGGAVFDILGKAINGKDLRQLLIEAIRYGERPDVRLRLSQK
                     VQEALDRDHLRELLEDRALVHESMDVTRVMQIRQEMERAEARRLQPHFIASFFFEAFK
                     HLGGTFKEREPDRFEIRHVPAAIRNRDRLIGFREPVQLRYERVCFEKDLIHVPGKPMA
                     SFICPGHPILDSTIDLILERYRHILKQGAVFLDENDMGEEIRVLFYLEHSIQDGVADK
                     AGNRRTVSRQLQFVEIDQHGNARSVGYAPYLDYRPPTEEESLLIKQTLKNQDWLRRDM
                     EAQATAHAIRHLVLNHLNEVKMRKQELVTKTMAAVKERLTKEITYWDHRAEELKVQEE
                     AGRINAKINSAKARLRADELTGRLQKRLAELELERQLSPLPPVVIGGALVVPKGLIKY
                     LQGDAGGEPGLFARKTLRVERMAVEAVMAMERSLGFDPRDMSAQKCGYDIESSIPGTG
                     QLRFIEVKGRTDGAETITVTKNEILTAMNKPEDFILAVVLVSASNLGLELGESTAGSF
                     PEAQEGQMPYDGSEQGCKVHYIRQPFRMEPDFGVTSINYDLARLLERAEAPL"
BASE COUNT         1691 a         2077 c         2000 g         1893 t
ORIGIN      
        1 gccctcacca tcaacttagc cgtgtacaaa ctgacaaggc cttgttcggc accccgctta
       61 agttccgcca aagaatacgt ctcgagctgc tcggcgctat cccaaatcac cagcagcacc
      121 tgcaatccct cacggagcgg ccagttccat atctccgacg gaagttcaac gcctttcttc
      181 agtgatcccc gtccaagcca gtagcgtacc agctcgctgc cttcccgatc ataccggatc
      241 cactgaaatg ggggctcagc tcttcgaata atctgcagat caggactgat gaaggatttc
      301 gtgacatcct ggtacttgaa gccctgattc tcaatccagt tgtggaagac atcgcgcaga
      361 tgttcttgcc tttgcttccg gtctccgtgt ccggctatgg gctctggctc tacgccactc
      421 tgagccggat ctacctcttc gatagcaggc gagtccgctg ctgttccagg tgggggaacc
      481 atgctgctgt gttgtgtttg gatctcctcg cccgtggata cgaatgagac ttccgaggtc
      541 tgcacttcct ccatgtctgt cctgagccgg atcacatcgt caatcttatc gtgaggctca
      601 ggagccctct ccatctcttc gtccagctca agatgctcat tagcataggc aattatccag
      661 gccggctccc tgtccgtaca gtcctgaacg gctctcttgg caacgacggt tcggaaaaaa
      721 cgagatatct caatgacaag atctcgatgg tgaaatgggg agctaccctg ggcatagatg
      781 gtatcgtcat gccatagagc cttgcatgat ttctccggtc cagccggttg tccatccatg
      841 tatggggcca gtttgagctc tgaaacagac tgccatccga ttcgtgataa acgggctgcg
      901 gcctttcggt cctcatcgta tgtgcacttc tgcgtgtttg catcagatcc gatgtcagga
      961 cagcgaagcc gcgaaaggat tcctccaagc gtctctatcc aagggggctt aggtgtgtag
     1021 ccagtaacgg cagcctttac gagacgcatc tctagagcct gatcgagagc ctccagaccc
     1081 atctctcggc acaaatcatg tgcaacatcc cctaacatgg tgaggtcagc ggtctttttt
     1141 ctaaaagatt caaagagact cagtccggat agatgcccga ctgctcccca tctcaaatcc
     1201 tcctttgaaa tccaccggcc cgatagattc aaccaggctc cacacttctg ccatacgatt
     1261 gcaggagcgc gctgaacaat ctgaaccacc ttctcacgat cggaagaatt gagtacttca
     1321 ttggatttct tctcgtttag ccactggaga gcggtttcca gggtaggttg cgctgccata
     1381 tccagacgtt gccacaagga gagatctttc aactctccgt ggatggtgct gatgccgggt
     1441 atgttttcag gattggactg gaagataaag gacagcttct cccaggtgca accatcggag
     1501 aatacgagcg cctcttcgga gaagatgtga cgcagttcgg ttaattcctc atgggaaata
     1561 cgcaatgtgg cacgatcgag cgctctaaat aggtctataa cgggagttac cggaacaact
     1621 gcggatcttg aaattgccct gatgcgatcg aggagggtac caaagccgga cggttggcat
     1681 cgtaccccca gcagatccag gaccttcgag tattcggggt gatcgagatc tggatgaaca
     1741 aatcgctcga tattcatgag cgccatcgtc tcggaagtgg ttcgaagaag ctctgcaggg
     1801 attgcggggc ggccgaaagt atccggtagg caaggcaggg atttcaatct aaggagccat
     1861 ccaacctcaa ggtcgccgtg gcccagtgaa tgagctttcg tgtgccccag ttgttttatg
     1921 gacgcatcga gtgtctcttc ccatttttcg gaccaccccc tgacgacgga aatcccaatg
     1981 gatttccaaa acgacggatc gtcctctgct ttctgattcc aatgttccca gagagcttcc
     2041 ttccagtcgt agtcacggag ctcaaaccag cggctttgta tgggatagga ggaaggggca
     2101 catcctcccc ttttgacgca aatattggag agcttctcct ttccatacaa ttcggttcga
     2161 gtgaggactg gcatgggaaa acagctcagc ttgctctttg tgacgtcact tgcccaatcg
     2221 cgccaggccc ctaagtattc ctgatcgagt cccgattcgt aagcgtccga caccacgaga
     2281 gagtcaaaga ggctttcggg aagtatttgg gacagatctt cggaattctc aacgatcaga
     2341 tgctcactgc aggctcgcca gcggccgtct ctgcagagga atttgaaact gtccgggact
     2401 ctgacaccgc ctcgtgccgc tacgtgagcc agtctgatag ctgcttcgcc tgggttttgg
     2461 gtgaagatcc tttctgcaac gaattttaca acctgttcga tgccaactcg ctcatgtaat
     2521 ttcaatttgt tgaacaggtc agatgccaga gcatgatctg aatccaaagg ggtgcccgtc
     2581 atgagcttca cccatttcgg attcacgata tcagcccagg acatcagaaa gctccagtcg
     2641 tcctcggata tcctcgactc tttgccgcca accgctatca gcttgccgga tggcatcaag
     2701 tcactacgct gggatgcagg cacgatcttg aggctctgta gctcattcca aaagttccag
     2761 ttgttccaaa gaggctgcag aaaattccaa aggaaaatga ggttttccaa tggctcaggg
     2821 cagggtggtc ccggattcat caggatcagt cttctggcga tgtccgttac cccaagccgt
     2881 tggaccagtg cccatgtttc caaggtctca ataaatcggg caggtatttc tcgtgcaatt
     2941 gcctttttct ttccttgccc aaaaacctga aggactttct ccgtacccca tgtgtcgtgg
     3001 acagcggccg gcaggtctat ggcttcacct ttttcagcca gagtaccatc atggcacagc
     3061 agcacctttt ggccgcttgt gatggctcgt ctgagacctt tcagtatgat tagagaagtc
     3121 tccttgttga gagatccatc actctccacc gactttggaa gcagctcata cgcgcgggca
     3181 cgttcaccga gcgacatcct gtcatttctt agccatccca gaatggcctc acctgccaat
     3241 tgcccgattc gttcaagcag ccacgcattg gtaggagaac tggctggatg cttgatttct
     3301 tttctggcag ggtcctgaac aaaaggagca ttaagagaaa aaggcatcaa gggctgcacg
     3361 tcggtgggca gcacagtgta gagacgggga tctggtgttc caaggatgat ttgaaccgcg
     3421 cacggaggta gatccagttc ttgggagccc ctctcttctc gaatctcttc gaggcattcc
     3481 ctcgggaatt gctcgggtgc tgagcgaaag cacaacagct cactctcttg ctcatttagc
     3541 aaccggattc tttccgagaa ggagactggc cccggtccga caatctcttt gtgaacaact
     3601 ctgccttgta tttcaaggct aacgatgctc tgaaaaaaaa gaagagggat cggactcttc
     3661 aaccatctct tgaactctgt ttcgagaacg gcacttttcg atgggccatc gagctgcact
     3721 ctgaaaatcg tttcaccttc agttgcaggg acattgatcc agcgaggctc ggtaaagcgc
     3781 ttcttctcaa aagcaaatgt gatcgttgga gtacgggctt caaccctggg gccgaaggca
     3841 aaaaccgagc gaaaaccgac gccacgaaaa ccgatggtat gcaaatgccg tttgtttgag
     3901 agaccaaatt ggcagagcga ttgaaaagtc acttcatcga agtcgttccc attgtgggcg
     3961 aactcgaaga ctccatcttt caaggaagcc cttacccatg ttgcaccggc gtcgtctgcg
     4021 ttttgcagga gctcggacaa tacatggcgg gggctttgta cctgcctgaa tagctgatgc
     4081 caggggcctg caaggacggg atcggattcc agctggtccc aacgcctgct cgcttccttg
     4141 ctgatacgga tgaaatactc gggtggagat acgatcataa cggagcctcc gccctttcca
     4201 agagtcttgc caaatcataa ttgatgctag ttactccaaa atcgggctcc atcctgaaag
     4261 gctgccgtat gtaatgcact ttgcaccctt gctctgatcc gtcataaggc atctgcccct
     4321 cttgtgcctc tgggaaggag ccggcagtgg attcacccag ttccaagccg agattagaag
     4381 ctgagacaag aacaacagcc agaatgaaat cttcaggttt attcatggcg gtaaggattt
     4441 cgttcttcgt cacggtgatg gtctcggcgc cgtctgttct tcctttgacc tcaatgaagc
     4501 gcagttgccc ggttcctgga atggaggatt cgatatcgta gccgcacttc tgcgcactca
     4561 tatctcgagg atcgaagccg agggaccgct ccatggccat cacggcttca acagccattc
     4621 gctcaacgcg gagggttttg cgggcgaaaa gccccggctc ccctccggca tcaccttgga
     4681 ggtacttaat caaacccttg ggaaccacca aggcgccccc tatgacgaca ggaggaagcg
     4741 gcgagagctg ccgttccagc tccagctcag caaggcgctt ctgaagtcgg ccggtgagct
     4801 catcggctcg caacctggct ttggctgagt tgatcttggc gtttatcctt cctgcctctt
     4861 cctggacctt cagctcctct gcgcgatgat cccagtaggt gatctctttg gtgagccgct
     4921 cctttaccgc ggccatcgtc ttggtgacca gttcctgctt gcgcatcttg acttcgttta
     4981 agtggttcaa cacgagatgc cttatggcat gtgccgttgc ctgggcctcc atatcccttc
     5041 taagccagtc ctggtttttc agtgtctgct tgatgagaag gctttcctct tcggtagggg
     5101 gccgatagtc aagatacggg gcataaccca cgcttctagc atttccatgc tggtctattt
     5161 ctacgaactg gagctggcgg gagacagttc gccggtttcc ggccttgtcc gccactccgt
     5221 cctggatgga gtgctccagg tagaagagta cccggatttc ttcacccatg tcgttttcat
     5281 ccagaaacac tgctccctgt ttgagaatat gacggtatct ttcaagaatg agatcgatag
     5341 tggagtcgag aataggatgc cccgggcaaa tgaaggaggc catgggtttc ccgggaacat
     5401 ggatcaggtc tttctcaaag cagaccctct cgtatcggag ttggacaggc tctctgaatc
     5461 ctatgagccg atcccggttc cggatggcgg ccgggacgtg gcggatctcg aatcgatcgg
     5521 gctctctttc tttgaatgtt ccgcccaggt gtttgaatgc ttcgaagaaa aaggatgcga
     5581 tgaaatgagg ttgaaggcgt ctggcttcgg ctcgctccat ctcctggcgg atttgcatga
     5641 ctcgagtaac gtccatcgat tcatgaacca gggctcggtc ttccagaagt tctcgcaagt
     5701 ggtctcgatc cagagcctcc tgaaccttct ggcttagccg caaccttaca tcaggccgct
     5761 caccgtagcg aatagcttcg atgagcagtt gccggagatc tttgccattg atggcttttc
     5821 caaggatatc aaagaccgct cctccaaggg cgatccgttc ggattctaat ttcctcaaaa
     5881 gagtgaggta gacatctcct tcgcgggtct cctccgccac gaggttccag aggtggcaga
     5941 cctcggtctg gccgatgcgg tggatgcggc cgaagcgctg ttcgatacga ttcgggttcc
     6001 acggaagatc gtagttcacc atgagatggg cgcgctgcag gttgatacct tcacccgcgg
     6061 catcggttgc taccaggatg agagtggatt tgtcctgctt aaatgcttct tcggctttct
     6121 tccgatcttc gcgtcccatc gctccgtgaa tagttataac agcttcaggt tttccgagga
     6181 gtgtgcggat gcgatctgtg aggtagttca gggtgtcccg gtgctctgta aagatgagga
     6241 gttttctgcg aaggccgttc gtgtcgaaca tctccggatt gttctgcaaa agtccggaga
     6301 gctcttccca tttcctgtcc tttccgctct gacgaacctg cagggcaagt gcctccaatt
     6361 ccttcagagt gcctatttcg gctttgagct ccgcaatggt tcttgccgca gtagcctggt
     6421 ccacgaattt ctcttcgatc tcctcaatct ctgcgttggg ggagtcttca agatcttcga
     6481 ggtcgtcttc ggtcacggcc ggcagatctg ctcccatcga aagccttgct tcggccccac
     6541 gctggagcag ctcctcttcg cgcagacgtc gctccaaccg ctcacgtcgc cttcgaagcg
     6601 actggtatat ggcttcaggc gaagaggcaa ggcgtctttg gagaatggtc agagcaaaac
     6661 cgacattacc ctttcgtttg tcatcgttca gagcatcagc cctgttgaac tcctcccgga
     6721 catattgagt gacccgctca tagagggctg cttcaaggtc ggagagcttg taggaaacgg
     6781 tataggcgag cctttcgggg aaaagcgggg ttccatcgaa cttatagagc tgctctttga
     6841 ccatccgccg catgagatcg gaaacatcca cagtatggac accatcacgg aatcttcctt
     6901 caaagcgatc gccgtcgagg agggccatga aaagctggaa atcttcctct ttgccgttgt
     6961 ggggtgtggc ggtcatcagg agaaaatgcc gtgtgatgct tgagaggagc tttccaagtt
     7021 tgtgcctctt agtttcctta acctctcctc caaagaagga agccgacatt ttatgggctt
     7081 catcgcagac aatgaggtcc caatcagtct gcatgagctt tccctgaagc tcttcattgc
     7141 ggctgagctg atccaaacgg aggataagta gattcatttc ttggaaagca ttgccggtcc
     7201 gtgctgtttc cagccgatcc ttcgtgagga tttcaaaagg caagaggaaa cgaccaaaca
     7261 gctcatcctg ccactgatcc gcaagatttc ccgggcagca gataaggcac cgctgcagat
     7321 cacctcgggc ggccaattcc tttatgaaca aaccggccat gatggttttg cctgcaccgg
     7381 gatcatctgc caggagaaat cggagaggct gacgcgtaag catctcaccg tagactgccg
     7441 tgatctggtg ggggagcgga tcaatgaggg aagtgtgtac ggcaagatac ggatcgaaaa
     7501 gatgggccat tcggatgcgg taggcctcgg agaccaaccg aaacagaccg gcatcggcct
     7561 caaaagccca gctgcggcct gtctggacta tttcaagcaa ggattcctga tcgcggaaga
     7621 gcagctcatt tgcaacccgc ccgcaattct ccttgtaggt a
//
LOCUS       DAOOVC010000178         7495 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5916_length_7495_cov_6.065188, whole genome shotgun sequence.
ACCESSION   DAOOVC010000178 DAOOVC010000000
VERSION     DAOOVC010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5916_length_7495_cov_6.065188"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="PLM79_15950"
     CDS             <1..1651
                     /locus_tag="PLM79_15950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOV87853.1"
                     /translation="TWGDFPGLLLFSCLALVAYIWTAPFRELYGLESRNALMAKEMLE
                     GGMGYLPTVHGRPYFDYPPLYFWLETLFSRLPGRITTFTAVLPSALAASGLVFLAGCF
                     GRLHGTRTAGLAAVLLATLPDFWIQASHATIDMLLAFHCGLAVYCFLRGEEGASRAGA
                     VLWTAGGLGAVAASFLSKGPIGIVLPAGIWCGYLVWGRRWKSLAVFVPILGVWAGVLT
                     GLHFSLLWKTGGGELVREVIRQQVFHRVVGEANKPLWYYPVHLAILVSPWLFRPASFA
                     WKAIRRDGGRDPFARVDTSRPGILLFRLAWIWVGVVFVIFSLAGSRHARYLLPLHLPL
                     CLLLAYAVEWTDENGDFPRGKGWERFFSILGWLVVGAGAAAFVLRMRDGQPPWVAGFL
                     WIAFSLGGRWGVRFLVPREGNRCTALLALGLATGLMGFQALWEPGLSVRESGRSFVEA
                     VESRASLELPVLLYGISADGDGIKFALYSRRPPGALRFVSSADDPESSPKPAILVFFE
                     KDRPTLSRVLSSCPWTELASGFLHSRPVTALWMEEPCGSSP"
     gene            2099..2950
                     /gene="panC"
                     /locus_tag="PLM79_15955"
     CDS             2099..2950
                     /gene="panC"
                     /locus_tag="PLM79_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOV87854.1"
                     /translation="MRIIELVSEMQQLADQWRRQGKRIGFIPTMGFLHEGHLALMREA
                     KNRADVVAVSVFVNPTQFGPNEDFERYPRDMQRDVRLCTDVGVDVIFAPSVPEMYPEG
                     FQTGIEVSKVTIPLCGRSRPGHFVGVATVVTKLFNSVKPHLAIFGQKDYQQWVVIRRM
                     VQDLNMDIEVVGHPTVREPDGLAMSSRNSYLSPDERQVALRLSRSLKAAQDLVDRGET
                     GGEVILEKVRDILDSGGGLRIDYVQLCHPDTLEEAPRVDEPAVLLLAAFVGKTRLIDN
                     CVLRPGK"
     gene            2990..3346
                     /locus_tag="PLM79_15960"
     CDS             2990..3346
                     /locus_tag="PLM79_15960"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOV87855.1"
                     /translation="MQRVLLKSKIHRAGVTEADLEYEGSLTIDEELMRAADILEFEQV
                     KVYNVNNGARFDTYAISGAPGSGIICLNGAAARMGAKGDRIIIATYGHYSEAEIREHR
                     PKLVFVDSHNRQIKKT"
     gene            3449..4610
                     /gene="metK"
                     /locus_tag="PLM79_15965"
                     /pseudo
     CDS             3449..4610
                     /gene="metK"
                     /locus_tag="PLM79_15965"
                     /inference="COORDINATES: protein motif:HMM:NF014791.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
     gene            4867..6123
                     /gene="ahcY"
                     /locus_tag="PLM79_15970"
     CDS             4867..6123
                     /gene="ahcY"
                     /locus_tag="PLM79_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOV87856.1"
                     /translation="MEFDIRDAALAEKGKLRIEWAARSMPVLRSIGERFAREKPLVGV
                     RLAACLHVTTETAALVQTLKAGGAEVRVCASNPLSTQDDVAASLVVHDAVPVFAVKGE
                     DKDTYYRHIHAALAHRPQITMDDGADLVSTIHAERKELLADIIGGTEETTTGVIRLKS
                     MAAKGVLKYPIVAVNDADTKHLFDNRYGTGQSTVDGIIRATNRLIAGSSFVVSGYGWC
                     GRGVAMRAAGMGAKVIITEVDPLRALEAVMDGYQVMSMAEAAKVGDFFCTLTGDIHVL
                     RKEHFQKMKDGAIVCNSGHFNVEIDLDALVRMAVGRRPIRDFVEEFQLSDGRRIYVLG
                     EGRLVNLAAAEGHPSSVMDMSFANQSLCSEFIVKNHRSLGRQVYRVPVDIDKEIARLK
                     LAAMGVRIDRLTAEQKKYLSSWEMGT"
     gene            6189..7394
                     /locus_tag="PLM79_15975"
     CDS             6189..7394
                     /locus_tag="PLM79_15975"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOV87857.1"
                     /translation="MPVRLWVLGLILLVSLPGNLGCVSQPPEKAGEPVGFEKVPGEDL
                     PGFCDDLDPASLRSALEKSLRYYGRIPPDRVLKLGDRELRAKWLKETLVRFLNLLDAG
                     ELNPGTIARSFDVYRVRFEGSPGRSLVTGYYEPILDGRLAPDQRFCYALYGLPPDLIT
                     IELSAFDSARFSGERLIGRLSGNRVVPYYTRSEIDGGRVLDPFGNQLAWLQDRVDVFF
                     LHVQGSGILRLPDNRHQRVGYAGANGRPYRSIGKYLREKGLLTGEITLQTIRAYLRAN
                     PERVQEVLWHNESYVFFRWVEEGPLGSINVPLTAGRSVAMDPGIHPAGAVLYLESQQP
                     KLDALGNVLRWEPMCRWVVNQDTGGAIKGLGRVDLFCGSGEAAERIAGRLQHPGKVYF
                     LLIKEIPQG"
BASE COUNT         1448 a         2153 c         2418 g         1476 t
ORIGIN      
        1 gacctggggg gacttcccgg gactgttgct cttttcgtgc ctggcactgg ttgcctacat
       61 ctggacggcc cccttccggg aactctacgg cctcgaatcc cgaaacgccc tcatggccaa
      121 ggagatgctc gagggtggaa tggggtacct tcccacggtt cacggccgcc cctacttcga
      181 ctatcctccc ctctacttct ggctcgaaac cctcttttcc agattgcccg gtcgaatcac
      241 caccttcacc gccgtcctac ccagcgccct ggccgcatcg ggcctggttt ttctggccgg
      301 gtgcttcggc cgtctccacg gcacgcggac ggccgggctt gccgccgtgc tgctggccac
      361 cctgcccgac ttctggatcc aagcgagtca tgcgaccatc gatatgctct tggcgttcca
      421 ttgcggtctg gcggtctatt gttttcttcg gggggaggaa ggggcttcaa gggcgggagc
      481 cgtcctgtgg accgcggggg ggctgggggc cgtggccgcc tcctttctca gcaaggggcc
      541 catcggaatc gtgctgcctg cgggaatctg gtgcgggtac ctcgtctggg gccgacgatg
      601 gaagagtctt gccgtcttcg tcccgatcct gggtgtctgg gcaggagtgc tcacaggcct
      661 gcatttttcg ctcctctgga aaacgggcgg gggggaactg gtccgagagg tgatccggca
      721 gcaggtcttc caccgggtgg tgggggaggc caacaaacct ttgtggtact accccgtgca
      781 cctggcgatc ctggtttccc cgtggctctt ccggccggct tccttcgcct ggaaggctat
      841 ccgcagggac ggagggcggg atccttttgc ccgggtcgat acctccagac ccgggattct
      901 cctgtttcgc ctggcctgga tttgggtggg ggtcgtgttt gtgatctttt ccctcgccgg
      961 ctcgcgccac gcaagatacc tcctccccct ccatcttccc ctttgtctgc tgctggccta
     1021 tgcggtggag tggacggacg aaaacgggga cttcccgcgg gggaagggat gggagcggtt
     1081 cttctccatt ctgggatggt tggtggtcgg ggcgggggcg gccgcttttg tccttcggat
     1141 gcgggacggg caaccgccgt gggtggcggg attcctatgg atcgcctttt ccttgggggg
     1201 gcgctgggga gttcgtttcc tggtgcctcg ggaagggaac cggtgcacgg cgctcctcgc
     1261 cctgggactg gccacggggc tcatgggctt ccaggccttg tgggaacccg ggctttcggt
     1321 cagggaatcg ggccgatcgt tcgtcgaggc ggtcgaatcc cgggcttccc tcgagttgcc
     1381 ggtgctgctc tacgggattt cagccgacgg ggacgggatc aagttcgccc tctacagtcg
     1441 gcgaccgccc ggggccctga ggttcgtttc ttcggccgac gacccggaat cgagtccaaa
     1501 acccgccatc ctggtgtttt tcgagaagga caggccgacc ctgtcccggg tgctctcctc
     1561 ttgtccgtgg acggaacttg cctccgggtt cctccattcg aggcccgtaa ccgccctgtg
     1621 gatggaagaa ccgtgcggat cgagcccgta gagtcctagg attccgggcg gtcgtcccgg
     1681 ggagagcggg tggggagggc ttcccggaaa tggagacttc tcggcgggca tcttcccgga
     1741 agaacgccga gggtcccgac ttcgggtcgg aggactcggc cccaagggag cccaaggcgg
     1801 gccgattgaa caggagtttc gggggtgagc ggattttcgt ccggggcaaa acctcccggg
     1861 tctctcgagg gaaagcccaa tgggattaat gacatagccc ttgacaaagc aagccctgcc
     1921 tgttagtgaa caggccagtt cagccagatt tcgctctgct tggatccaaa gaggactgag
     1981 acagaaagga tctcaagatg aagccggagc gccgaaaacg gcgccggcgc acaaagggag
     2041 ccttccggac tgacctcgcg aaggctttta acattttggc ggcaagaagg aggaatccat
     2101 gcgcatcatc gagctcgttt ccgagatgca gcagctggcc gaccaatggc gaaggcaagg
     2161 aaaacgcatt ggttttatcc ccaccatggg ctttttgcac gagggccatc ttgcactgat
     2221 gcgggaggcc aagaatcggg ccgatgtggt ggcggtcagt gttttcgtga atcccaccca
     2281 gtttggaccc aacgaggact ttgagcggta tccccgggac atgcaacggg atgtgcggct
     2341 ttgcacggac gtgggggtgg acgtgatctt tgccccctcg gtcccggaaa tgtaccccga
     2401 aggttttcaa accggcatcg aggtatcgaa agtcacgatt cccttgtgcg gtcgaagtcg
     2461 acctgggcac ttcgtggggg tcgccaccgt ggtgaccaag ctcttcaaca gcgtcaaacc
     2521 gcacctggcg attttcggac agaaggacta ccagcagtgg gtcgtcatcc ggagaatggt
     2581 tcaggacctc aacatggaca tcgaggtcgt ggggcatccg acggttcgcg aacccgacgg
     2641 gttggccatg agcagccgaa atagctattt gagcccggac gagcgacagg ttgcccttcg
     2701 cctgagccga tccctcaagg cggcccagga cctcgtggac aggggggaaa ccggcgggga
     2761 agtgattctg gaaaaggttc gagacatctt ggactcaggg ggaggactcc gaatcgatta
     2821 tgttcagttg tgccacccgg acactttgga ggaagcgccg cgggtggatg agcctgcggt
     2881 gctcctcctg gctgcgtttg tcgggaaaac tcgcctcatc gacaactgcg tgctgcgacc
     2941 gggaaaataa tctcttgaag aagcgattgc tgaacatacg gaggggccca tgcaacgagt
     3001 actgctcaag tccaagatcc atcgagccgg ggtcacggaa gccgacctgg aatatgaagg
     3061 cagcctcacc attgatgagg agttgatgag ggcggccgac atcctggaat tcgaacaagt
     3121 gaaggtctac aacgtcaaca acggtgcccg ttttgacacc tatgccattt ccggtgcgcc
     3181 cgggagcggg atcatctgcc tcaacggggc tgcggcaaga atgggagcca aaggcgaccg
     3241 gatcatcatc gccacgtacg gtcattattc cgaagcggag atccgagaac accggccaaa
     3301 actggtattt gtcgactccc acaaccgaca gatcaagaaa acttgatgtt ccccggcaag
     3361 ccggaaggaa aacgccggcg cccagcgggg tctctcaacg gcccgtactt aaagattcaa
     3421 aagcgagagg agaaaaccct ttatgtccat gaagaacttc ttgttcacgt cggaatcggt
     3481 tgcggaagga caccccgaca aggtggcgga ccagatttcc gacggcattt tggatgccat
     3541 catcgcagag gacaaggcgg cgcgggtggc gtgcgaaacc ctggtcacca cgggactcgt
     3601 cttcctggcc ggggagatca ccacctcttc ctggatcgac atcccggaaa tcgtccgcac
     3661 caccattcgc gacatcggct acaacgattc ctccatgggc ttcgactggg aaacttgtgc
     3721 ggtgatcacc agcatcgaca agcagtcccc ggacatcgcc atgggtgtga accctggaga
     3781 gggcctgttc gaagaacagg gcgccgggga ccagggcctg atgttcggct atgcctgcga
     3841 cgagacccca gtgctcatgc ccatgcccat ctactgccca ccgcatctgc cgaaagctcg
     3901 ccgaggtgag aaagagcggg gtgctgggtt ttctcaggcc cgacggcaag agccaggtga
     3961 ccgtcgagta cgacgaccac aggccggtga ggatcgacac cgtggtggtg gcggcccagc
     4021 atgctcccaa cgtctcctac aacaccatcc gggaatccat catcgaggaa gtcatcaaga
     4081 agaccatccc ggcggaactg ctggacgaca agaccaagtt ttacatcaat tccaccgggc
     4141 gtttcgtggt gggaggaccc ttggccgact gtggaatgac ggggcgaaaa atcattgccg
     4201 atacctacgg cggtcagggc agtcatgggg gaggggcctt ctcggggaaa gaccccagca
     4261 aggtggatcg tacggcctcg tacatggccc ggtacgtggc taagaacatc gtggccgccg
     4321 gactggccga ccgggtcgag gtccaggtgg cgtacagcat cggcgtggcc gagcccgttt
     4381 ccctcatgat tgactccttc gggaccagca agatctcccc cgaccgaatt gcccaaatcg
     4441 ttcgagaggt tttcagtttc aaacccgcca acatgattcg gcacctgaag cttctccggc
     4501 ccattttcaa gaagacttcc tgctacggcc atttcggtcg caatgacccg gacttcacct
     4561 gggagaagac cgatatggtg gacgttctca gggacaaggc cggcttgtag gggggctgct
     4621 cagcatttca cccgcatgga accgccggga attcgtttcc cacgggccaa tgccggttcc
     4681 cgtttgtcgg ggccggcatt gccgggttca aagggtgaaa cccggtggcg caatttgacc
     4741 cagaatggat ccgtgcctga caccaaacga cccagggggt cctgggggcc ggccccgtcg
     4801 tgggggcaag ccgggtttcc ccggggccga ggcggcggga ccaccacatt tttggaagga
     4861 gtttccatgg aattcgacat cagggatgcg gcgttggcgg agaagggaaa actgcgaatc
     4921 gaatgggcgg ctcggtctat gcccgtgctt cgatccatcg gggagcgctt tgcgcgggaa
     4981 aagcctttgg tgggagtccg cctggccgcc tgcctccacg tgaccactga aacggccgcc
     5041 ctcgtgcaga ccctcaaagc cgggggagcg gaggttcgag tttgtgcgtc caaccccttg
     5101 agcacccagg acgacgtggc ggcctctctg gtggtccacg acgccgttcc cgtttttgcc
     5161 gtcaagggag aggacaagga cacctactac cggcacattc acgccgccct ggctcataga
     5221 ccccaaatca ccatggacga cggtgcggac ctggtgagca cgatccatgc ggagaggaaa
     5281 gagctcctcg cggacatcat cgggggaacg gaagagacca ccacgggggt cattcgtctc
     5341 aagagcatgg cggcaaaggg cgtgctcaag tatcccatcg ttgcggtgaa cgacgcggac
     5401 accaagcacc tgttcgacaa tcgttacggt acgggccaga gcaccgtgga cgggatcatt
     5461 cgggccacca atcggttgat tgccggatct tccttcgtgg tgagcggata cggctggtgc
     5521 gggcgggggg ttgccatgcg tgcggcgggc atgggggcca aagtgatcat tacggaagtg
     5581 gatcccctgc gggccctgga ggcggtcatg gacggctacc aggtcatgtc catggccgaa
     5641 gccgccaagg tgggcgactt tttctgcacc ctgacgggcg acattcatgt cctcagaaaa
     5701 gagcatttcc agaaaatgaa ggacggcgcc atcgtttgca actcaggcca cttcaacgtc
     5761 gagatcgacc tggacgccct ggtccggatg gccgtgggcc gaaggcccat tcgggatttt
     5821 gtggaagagt ttcaactgag cgacggccgc agaatctacg tgctgggaga gggcaggctg
     5881 gtcaatctcg cggcggcgga aggacatccc tcgagcgtca tggacatgag ttttgccaac
     5941 cagtccctgt gttccgagtt catcgtgaag aaccacagga gcctgggcag gcaggtctat
     6001 cgggtccccg tggacatcga caaggagatc gcccggctca agctcgccgc catgggcgtc
     6061 cggattgatc ggctgacggc cgagcagaag aagtacctga gctcgtggga gatggggacc
     6121 tagccgaccc gagggatcgg acccggcaat cccccgatcg cgtcggcgga cgggacggag
     6181 cgttcatcat gcccgtgaga ctttgggtat tggggctcat cctcctcgtt tccctcccgg
     6241 ggaacctcgg ttgcgtttcc caaccacccg agaaagcggg tgagcccgta gggttcgaga
     6301 aggtccccgg ggaggacctg cccggttttt gcgacgacct cgatcccgcc tccctgagaa
     6361 gtgccctgga gaaaagcctt cgctactacg ggcgaatccc cccggatcgg gtcctcaagc
     6421 tgggggaccg ggagcttcga gcgaagtggc tcaaggagac gctggttcgc ttcctcaacc
     6481 tgctggatgc gggagagctc aaccccggca cgattgctcg gtcgtttgat gtgtatcgag
     6541 tgcggttcga ggggtccccg ggacggtcgc tggtgaccgg ctattacgag ccgattctgg
     6601 acgggcggct tgcacccgat cagcgatttt gttatgcttt gtatgggttg ccaccggatc
     6661 ttataaccat tgaactttcc gcctttgatt ctgcccggtt ttcgggagaa cgcctcatcg
     6721 gacgcctttc ggggaatcga gtggtccctt attacacccg atcggaaatc gacggaggga
     6781 gggttctgga tcccttcggc aatcagcttg cctggttgca ggatagggtg gacgtgttct
     6841 ttcttcatgt gcaggggtcc gggatcctcc ggttgccgga caatcggcat cagagggtcg
     6901 gttacgcggg ggccaacggc cgcccctatc ggagcatagg caagtacctg cgggaaaagg
     6961 ggctgctgac gggagagatc accctgcaga ccattcgtgc ctacctgcgg gcaaaccccg
     7021 agagggtgca ggaagtcctg tggcacaatg agagctacgt cttttttcga tgggtcgagg
     7081 aagggcccct gggaagcatc aacgtacccc tgacggccgg ccggtcggtg gccatggatc
     7141 ccggcattca ccccgccgga gccgtcttgt acttggaatc ccaacagccc aagctcgatg
     7201 ccctggggaa cgtgctccga tgggaaccca tgtgtcgatg ggtggtaaat caggacacgg
     7261 gaggagccat taaggggctt ggtcgggtgg atctgttttg cggcagcggg gaggctgccg
     7321 aaagaatcgc cgggaggctc cagcacccgg gtaaagtgta tttcctgctg atcaaggaaa
     7381 taccgcaggg ataagaatca tgtaagctcc gcggattctc tgttatactc cagcaagaac
     7441 tgaaacagga ttctcatttc gtcgaaacac cgacacaagt ccgtggccag gcgga
//
LOCUS       DAOOVC010000179         7473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5937_length_7473_cov_8.052844, whole genome shotgun sequence.
ACCESSION   DAOOVC010000179 DAOOVC010000000
VERSION     DAOOVC010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5937_length_7473_cov_8.052844"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLM79_15980"
     CDS             <1..1183
                     /locus_tag="PLM79_15980"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOV87858.1"
                     /translation="RVVDRKKAGDHLVVVLSARAGETDQLIRLARETSPSPDPRELDV
                     LLATGEQITIALFSMAVKDLGMDAVSMTGYQAGIMTSREYGQARIHWIETQPIVEKLN
                     EDKIVVVAGFQGYDDEGNITTLGRGGSDTTAVALAAALGAEVCEIYTDVEGVFTADPN
                     IYSKARKLDKISYEEMLEMASMGAKVLHLRSVEFGMKYNVPIMVLSSFTDSPGTLVTK
                     EDEDMEKVVVSGITYNKNEARITVTNVPDKPGMAAKLFRPISDANVNVDLIVQGSGSI
                     PGRANISFTVPKPHYEKTLEIAREVAREIEAGPVHSNPSIAKISIIGLGMRSHSGVAS
                     KMFETLSRENINIKMITTSEIKISCVIDEKYTELAVRVLHDAFQLEAEPQRNIKIQEE
                     V"
     gene            1234..2823
                     /gene="cimA"
                     /locus_tag="PLM79_15985"
     CDS             1234..2823
                     /gene="cimA"
                     /locus_tag="PLM79_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOV87859.1"
                     /translation="MREVKFYDTTLRDGTQAEDFVLSLQDKLRIALKLDDLGIHYIEG
                     GWPGSNPKDAAFFKEIRNYTLKNAQIASFGSTHHPTKTAETDPNLKALLSTRTPVVTI
                     FGKSWLVHVRDALHITPERNLELIAGSLRYLRPQAKTLFYDAEHFFDGFKDNAEYALA
                     TLQTALEAGADCLILCDTNGGNLPATIQQTMKVVKERLPDAPLGIHAHNDSELAVANS
                     IAAVEMGAVQVQGTINGVGERCGNANLISIIPTLCLKMGIPSLAPENLRNLRRVSRFV
                     LELANVTPNRYQPYVGRSAFAHKGGIHISAVEKNPLTYEHIDPELVGNRRRILVSDLS
                     GRAAIKRKAKDFGIDLSKQDPVALQILEDVKDLENRGFQFEAAEASLELLINRAMGRG
                     KRYFELVGFRVVDQKLNENDDPVAEATIRVKVGGNIEHTAALGNGPVNALDNALRKAL
                     EKFYPQLKEMELSDYKVRVLPGRDGTAAKVRVLIESQDSSDQWGTVGVSHDILEASWQ
                     SLVDSINYKMYKDEKQKSAKC"
     gene            2817..3392
                     /locus_tag="PLM79_15990"
     CDS             2817..3392
                     /locus_tag="PLM79_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOV87860.1"
                     /translation="MLKSFLSRSQWAAITLVAACASLLLLVRLHREDHPPQPGPSPVV
                     FELRGNLSRPGVYFADGPRLTLREALQMGGGLTEGNPPEISRELLDSEIPTGQTLHVS
                     RNPSGEWTVRRDAMSAGARFILGEKLDINRDSEEDLALVPGMRPHFAAAIVRRRAREP
                     WKKVEDLAEIPGVGPKTLEKWRGYLAVPRTP"
     gene            complement(3515..4318)
                     /locus_tag="PLM79_15995"
     CDS             complement(3515..4318)
                     /locus_tag="PLM79_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOV87861.1"
                     /translation="MAVLTISREIEVEGTEIGRSVAETLGYEYWSGAKILERLDARGD
                     QWKKWAESLDNHCPTVWERYDWSFKSFAALLQSALLDCAAQGRAVLVGKGGNFLLGDV
                     PFCLRVHLVGSPRWLARAVMRQEGTDEKTAAWLVEKKDRERSCFLHSLYGRNWEDPKE
                     YDRVFNPEIQPMGEILSTVLDLLKEREKLQTKEAMETLRLRARAARARARILTDPRML
                     LPTLEIALEGDGLVLRGIVHNPEEHRKLERAARELAGEVRLKCDLRYRQ"
     gene            complement(4326..5078)
                     /locus_tag="PLM79_16000"
     CDS             complement(4326..5078)
                     /locus_tag="PLM79_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOV87862.1"
                     /translation="MDLGIFGSIHLNWQFFSALFSIVIIDLILAGDNAVVIAMAVRSL
                     PHHQRRKGILFGAGAAVLLRVVLTFFVAQLLQVNYLKLAGGVLILWIAVKLFVEGSPE
                     ESIHKEAATIGQAVKIIVIADITMATDNMLAVAGASHGNLFLLLFGLGLSIPFVVFTS
                     NLLSMLMDKYPVILYLGAAILGKVGGEMILTDPIIFHYLQPGKYLQYGVEAAFAVGVI
                     VVGKLWIKWMIRSGPEHAHEEAEVSLNPERND"
     gene            5391..7151
                     /locus_tag="PLM79_16005"
     CDS             5391..7151
                     /locus_tag="PLM79_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOV87863.1"
                     /translation="MAENDVKKPAEPDYVVIESGKTTLKDLYTKEDWMAIWMGFLVLI
                     VGLLIFLPRPPEKMNENLAKYNATMKEEAAKAPFKTVAWHQASSAKKGIRARDQAYGK
                     TIREFLDAPGDWTTNPLDALYRSKETADAMNAAAKGAYDKAKAAEDAALAQAKAAEAA
                     AAAAGFKDASLNSEAEKQISAWLAAKDKASKAKSKANVKPFNRVPYLAGLAVILGIFF
                     GIGRAIMGFSFGRFFVGFFAVFVLAVLAYMAETQATMKHWGFGFPLWAIVFGMLISNT
                     IGTPKWVMHAAQTEFFIKTGLVLLGAEILFNKILAIGKPGIFVAWVCTPITLVLTYWF
                     GQRVIKMASKTLNITISADMSVCGVSAAIATAAACRAKKEELTLAIGLSMVFTAICMV
                     AQPAFAKLVGMPQILAGAWMGSTIDSTGAVAAAGAFYGEKALYVAATIKMIQNVLIGV
                     TAFCVAVYWCAKVECAPGQTVSWWEVWYRFPKFVLGFMTASIVFSFIDQSMGKDLSTI
                     MIDQGVLRGFTRIAREWFFALAFASIGLETNFREFKPYFKGGKPITLYVFGQTFQLCL
                     TLLVGYIMFYLVFPEVTASL"
     gene            7153..7446
                     /locus_tag="PLM79_16010"
     CDS             7153..7446
                     /locus_tag="PLM79_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87864.1"
                     /translation="MTIPREDRSESPRRNGSLRRGLNPWWKLVLGIALLLAFFLVVAP
                     LSKHLPGARRMGEIIDERNLRATAIYYTDLEETAEGSAAIRGSLEYPPTPPRP"
BASE COUNT         1613 a         2268 c         2142 g         1450 t
ORIGIN      
        1 acgcgtggtg gatcgcaaaa aggcggggga tcacctggtg gtagtcttat cggctcgggc
       61 gggggaaacg gaccagttga tccgcctggc ccgggagacg agcccttccc cggacccccg
      121 agagttggat gttctgctgg caacggggga acagatcacc attgctttgt tcagcatggc
      181 cgtcaaggac ctgggaatgg atgcggtttc catgaccggc taccaggccg ggatcatgac
      241 cagccgggaa tacggtcagg cgcgcatcca ctggatcgag acccaaccca ttgtggagaa
      301 actgaacgaa gacaagattg tcgtggttgc cgggttccag ggatacgacg acgaaggaaa
      361 catcaccacc ctgggccgcg ggggatccga cacgacggcg gtagccctgg ccgcggccct
      421 gggggccgag gtgtgcgaga tctacacgga cgtggaaggg gtcttcaccg ccgatcccaa
      481 tatttactcc aaggcaagaa aactcgacaa aatcagttac gaagaaatgc tcgagatggc
      541 cagcatgggg gccaaggtgt tgcacctgcg gtccgtggaa ttcggcatga aatacaatgt
      601 gcccatcatg gtgctctcgt ccttcaccga ttcgcccggg accctggtta ccaaggagga
      661 tgaagacatg gaaaaagtgg tggtttcagg aatcacctac aataagaacg aggcccgcat
      721 caccgtcacc aatgtccccg acaagccggg catggcggcc aagcttttcc ggcccatatc
      781 cgacgccaac gtgaatgtgg acctcatcgt tcagggaagc ggaagcattc ccggccgggc
      841 caacatctct tttacggtcc ccaaacccca ctatgaaaag accttggaga tcgcccggga
      901 agttgcccgg gaaatcgagg ccgggccggt tcacagcaat cccagcattg ccaagatctc
      961 catcatcggg ctgggaatga gaagccacag cggggttgcc agcaagatgt tcgagaccct
     1021 gtcccgggag aacatcaaca tcaagatgat cacgacctcg gagatcaaga tttcctgtgt
     1081 catcgacgag aaatacaccg aactggccgt ccgagtcctc cacgatgcat tccagctgga
     1141 agcggaaccc cagagaaaca tcaagatcca ggaggaggtc tgaagacccg gggccgactc
     1201 ctccggcctt gatccttaag gaaggcgatc cccatgcgag aggtcaagtt ttacgacaca
     1261 acccttcggg acggaaccca ggcagaggac tttgtcttgt ccctccagga caagttgcga
     1321 atcgccttga aactggacga cctgggaatc cactatatcg agggaggctg gccgggctcc
     1381 aaccccaagg atgcggcgtt cttcaaggaa atccgaaatt acaccctcaa gaacgcccaa
     1441 atcgcctcct tcggttccac ccatcacccg accaagacgg ccgagacgga cccgaacctc
     1501 aaggccctgc tctccaccag gacccccgtg gtcaccatct ttggaaagtc ttggctggta
     1561 cacgttcggg atgcgctcca catcaccccc gagcgaaatc tcgagctcat tgccggcagc
     1621 cttcgctacc tgcgacccca ggccaagacc ctcttttacg acgccgagca cttctttgac
     1681 gggttcaagg acaacgcgga atacgccctg gcgaccttgc agaccgccct cgaggcaggg
     1741 gccgactgcc tgattctgtg tgataccaac ggaggcaatc ttcccgccac cattcagcaa
     1801 accatgaagg tcgtcaagga acgattgccc gatgctcccc tgggcatcca tgcccataac
     1861 gactcggaac tggccgtggc caactccatc gccgccgtcg agatgggagc cgtccaggtc
     1921 caggggacca tcaacggggt tggggaacgg tgcggaaacg ccaacctgat ctccatcatt
     1981 cccaccctgt gcctgaagat gggaattccg tccctggcgc ccgagaacct tcgaaacctt
     2041 cgacgggtga gccgtttcgt attggaactg gccaacgtga cccccaaccg ttatcagccg
     2101 tacgtgggca gaagcgcttt cgcccacaag gggggaatcc acatcagcgc cgtcgaaaag
     2161 aatcctctca cttacgagca catcgacccg gaattggttg gaaatcggcg aagaatcctc
     2221 gtctccgacc tttcggggcg ggcggccatc aagagaaagg ccaaagattt cggcatcgat
     2281 ctgtccaagc aggacccggt ggccctgcag atactcgagg acgtcaagga tctcgaaaac
     2341 cggggtttcc agttcgaagc ggcggaggcc agtctcgagt tgctcatcaa ccgggccatg
     2401 ggaaggggga agcggtactt cgaactggtg ggattccggg tggtggatca gaagctgaac
     2461 gaaaacgacg atcccgtggc cgaggccacc atacgggtca aggtcggcgg aaacatcgaa
     2521 cacacggcgg ccctgggaaa cggacccgtc aacgccttgg acaacgcgct gcgcaaggcc
     2581 ctggaaaaat tctaccccca actcaaggag atggaactca gcgactacaa agtgcgggtc
     2641 ctgccgggac gggacgggac ggccgccaag gtgcgggtgc tcatcgaatc ccaggattcc
     2701 tcggaccagt ggggaaccgt gggcgtttcc cacgacattc tcgaagcaag ctggcagtcc
     2761 ctggtggaca gcatcaacta caagatgtac aaggacgaga aacaaaagtc agccaagtgt
     2821 tgaagtcctt tctttcccgc agtcaatggg ccgccatcac cctggtggcg gcctgcgcct
     2881 ccctcctgct cctggttcgc ctccaccggg aagaccaccc ccctcaaccg ggtccgtcac
     2941 ccgtcgtgtt tgaactccgg gggaacctct cccgcccggg agtctatttc gccgacggac
     3001 cccggcttac cctccgggaa gccctgcaga tggggggagg cctcaccgaa ggaaatccgc
     3061 cggaaatctc gagggaactc ctggacagcg aaatccccac cgggcaaacc cttcacgttt
     3121 cccgaaatcc ttcgggagag tggaccgttc gtcgggacgc catgagcgcc ggggctcgtt
     3181 tcatcctggg agaaaaactg gacatcaatc gggactcgga ggaggacctt gccttggttc
     3241 ccggaatgcg gccccatttt gccgcagcca tcgtgcgccg ccgcgcccgc gaaccctgga
     3301 agaaggtcga agacctggcg gaaattcccg gggtcggccc caagaccctg gaaaagtggc
     3361 gaggttacct ggccgttccc cgcacaccgt gattccaatc cctaaaaacc gcggatcagg
     3421 gcggacgatc gcccgtttcc gtgccccgtt atccgggatc aagccgggga acggccgggt
     3481 ccggggttgt cacgaagaaa ggtcgccgga tttctcactg ccgataccgg agatcgcact
     3541 tgagccgaac ttctcccgcc agctcccttg ccgcccgttc cagttttctg tgttcttcgg
     3601 ggttgtggac gatccccctg aggaccaggc cgtcgccttc gagagcgatt tcgagggtgg
     3661 gcaggagcat gcggggatcg gtgaggattc gggccctggc cctggccgcc ctggcccgca
     3721 accgcagggt ctccatggcc tccttcgttt gaagtttttc ccgttccttg aggagatcga
     3781 ggacggtgga gaggatttcc cccatcggct gaatctcagg gttgaagacc ctgtcgtatt
     3841 ctttgggatc ctcccaattc ctcccgtaca gactgtgcag gaagcaggac ctctcacggt
     3901 cctttttctc caccagccac gcggcggtct tttcgtccgt cccttcctgc cgcatcacgg
     3961 cccttgcaag ccatcgggga gaaccgacca ggtgaaccct caggcaaaag ggaacgtctc
     4021 ccagaagaaa gttgccccct ttgcccacga ggacggctcg gccctgggcc gcacaatcca
     4081 ggagggcact ctggagcagt gccgcaaaac tcttaaagga ccagtcgtat ctttcccata
     4141 ccgtcgggca atggttatcg agggattcgg cccatttctt ccattggtcc ccccgggcat
     4201 ccagcctctc caggattttt gccccgctcc agtactcgta gcccaaggtt tcggccacgc
     4261 tgcgaccgat ttccgttcct tcgacttcga tttcccggga tatcgtcaag acggccatag
     4321 gcttctcaat cgttcctttc agggtttaag gatacttccg cctcttcatg ggcatgctcg
     4381 ggtcccgaac ggatcatcca tttgatccac agcttgccca ccacgatcac cccgacggca
     4441 aatgccgctt cgaccccgta ctggagatac ttgccgggct gcaaatagtg gaagatgatg
     4501 gggtccgtaa ggatcatctc cccccccacc ttccccaaga tggcggcacc caggtaaagg
     4561 atgacggggt acttgtccat gagcatggaa agaagattgc tggtgaagac cacaaacggg
     4621 atgctcaacc ccaaaccgaa caggaggaga aagagattgc cgtgggatgc ccccgccacg
     4681 gccagcatgt tgtccgtggc catggtaatg tccgcaatga cgatgatctt gaccgcctgc
     4741 ccgatggtgg cggcttcctt gtggatggat tcttcgggag acccttccac aaagagcttg
     4801 acggcgatcc agagaatcaa caccccgcct gccagcttca ggtaatttac ctggaggagt
     4861 tgggcgacaa aaaacgtgag gacgactctc agcagaaccg ccgccccggc gccgaacagg
     4921 attccctttc gccgctgatg gtggggaaga gacctcacgg ccatggcgat gaccacggcg
     4981 ttgtctccgg cgaggataag atcaatgatc acgatgctga agagggcgga aaagaactgc
     5041 caattcagat gaatggaacc gaatatccct aagtccaatc ctttgatctc ctttcttgaa
     5101 ggtgtataca aataagtgcg gctactatat tcgctaggtt ccggcccgtc cagtccaaac
     5161 attcggcacc gcccggcctt cggccgccgg agattctccc cttcggtttc aatcctttcc
     5221 cctgcgggct ccgcgccctg aatagagtat tgtcaagatc ccccgtgcgg atgtagtatg
     5281 gctccggtgt agagaattct tctcaccatt tttgttggat cgtttatggt tctggatgaa
     5341 ttttcttcgg tttcatcttc gaccctccgg ggtccgacag gaggaaggtt atggcggaaa
     5401 acgatgtgaa gaaacctgca gaaccggatt atgtagtgat cgagagcggc aagaccactc
     5461 tcaaggacct ctacaccaag gaagactgga tggccatatg gatgggattt cttgttctca
     5521 tcgtcgggct cctgatcttc ttgccgcgac ctcccgaaaa gatgaatgag aaccttgcca
     5581 agtacaacgc caccatgaag gaagaagccg ccaaggctcc tttcaagacc gtcgcgtggc
     5641 accaggcgtc ttccgccaag aagggaatcc gggcccggga ccaggcatac ggcaagacga
     5701 tccgcgagtt cctggatgct cccggcgatt ggaccacgaa ccccctggac gctctttacc
     5761 gcagcaaaga aacggccgac gccatgaacg cggcggccaa gggggcctac gacaaggcca
     5821 aggcggcgga agatgcggcc ctggcccagg ccaaggcggc ggaagcggcc gcggccgcgg
     5881 cggggtttaa agacgcgtcc ctgaacagtg aggcggaaaa acagatctcc gcatggctgg
     5941 cggccaagga caaggcttcc aaggccaaga gcaaggccaa cgtgaagccg ttcaatcgcg
     6001 ttccctatct ggccggcctg gccgtcatct tggggatctt cttcggaatc ggtcgagcca
     6061 tcatgggatt ctccttcggc cgtttctttg tgggtttctt cgccgtcttt gttctggccg
     6121 tccttgccta catggccgaa acccaggcaa ccatgaagca ttggggattc ggcttcccgc
     6181 tctgggccat cgtctttgga atgctcatca gcaataccat cggcaccccc aaatgggtga
     6241 tgcacgccgc ccagaccgag ttcttcatca agaccgggtt ggtcctgctg ggagcggaga
     6301 ttctcttcaa taagatcctc gccatcggaa agcccggcat ctttgtggcg tgggtgtgca
     6361 cgcccatcac gctcgtcctt acctattggt tcgggcaacg ggtgatcaag atggcctcca
     6421 agaccctgaa catcaccatt tccgccgaca tgtcggtgtg cggcgtttcg gcggccatcg
     6481 ccacggcggc ggcttgccga gccaagaagg aagaactgac cctggccatc ggcctctcca
     6541 tggtctttac ggccatctgc atggtggctc agcccgcctt tgcaaagttg gtcggaatgc
     6601 cacagatcct tgcgggggcc tggatgggaa gcaccatcga ttcgacggga gccgtggctg
     6661 cggcgggcgc cttttacgga gaaaaggccc tgtacgtggc ggcaaccatc aagatgatcc
     6721 aaaacgtgct gatcggcgtc acggcgttct gtgtggcggt atactggtgc gccaaggtgg
     6781 aatgcgcccc cggccagacg gtaagctggt gggaggtctg gtatcgattc cccaagttcg
     6841 ttctggggtt catgacagct tcgatcgtct tctccttcat cgatcagagt atgggaaagg
     6901 acctcagcac catcatgatc gatcaagggg tcctcagagg tttcacccgg atcgcccggg
     6961 aatggttctt tgccctggcc ttcgcctcca tcggtcttga aaccaacttc agggagttca
     7021 aaccctattt caagggagga aagcccatta ccctgtacgt ctttgggcag accttccagc
     7081 tctgcctgac tctgctggtg ggctacatca tgttctatct cgtcttcccc gaggtcaccg
     7141 cgagcctgta ggatgacgat tccaagggaa gaccgctcag aaagcccccg ccgcaacggt
     7201 tctttgcggc gggggttgaa cccctggtgg aagctggttt tggggatcgc cctgctcctg
     7261 gcattcttct tggttgtggc ccctctcagc aaacacctgc ccggggctcg aaggatgggg
     7321 gaaatcatcg acgaacgaaa tctccgggca acggccatct actacacgga cctcgaggag
     7381 acggcggaag gctcggccgc catccgaggc agcctcgagt accctcccac acccccccgg
     7441 ccataagggg ccttcgtgct ccccaaggag cgc
//
LOCUS       DAOOVC010000180         7442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5973_length_7442_cov_6.110600, whole genome shotgun sequence.
ACCESSION   DAOOVC010000180 DAOOVC010000000
VERSION     DAOOVC010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5973_length_7442_cov_6.110600"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            502..3450
                     /locus_tag="PLM79_16015"
     CDS             502..3450
                     /locus_tag="PLM79_16015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87865.1"
                     /translation="MKNRGYHRLRVHVITLILVVPAFVVFYEIRNLTEDYHQTIRSAQ
                     TRLASYASGLSEHATVTLYSAEIALKYVVDRVQEHGLLEPPDEWKIHELLRDAARGYP
                     QFGSLLMVGCDGKLVANSLEFPVIEVQLSDRDYFRFHLEHESTTEIHISKPVMNRLNG
                     LWRFFMTRRLSKRDGSFGGIAGVALDPAYYDRFYQTIDVGKSGRILLIRIDGRVLMQT
                     PFSPEALEVDYAQRPMLAEHLPSFPWGTYRNPTAGFDSTDRLISYHRLEKYPVVATVS
                     MLVDEILAPWRVRLRNGFINLGILLLLVAGLGSLLWNRLRDLEKVQASLLEKQEELRE
                     SQRAMDTLFNNLPGMAYRRSADPDWTLRFASEGAGSLTGYRPAELTQSPQASFFSLIH
                     REDAISARNAVQSALADKRSYRVQYRLISRDQQERWVWDAGTGVWDEAGRVKAMEGFI
                     TDVSELRAVEQALRRSEEKYRTLFEEAINGIVLADARTGIVLDCNRAALAILERSKDQ
                     VVGKPWQPLSPRQSRDDGTASELQLNPGPPLDKMIETKVITPSGANKEIALKASRVLV
                     EGRELVQCIFRDVTEAKKLESRLQQARKMEAIGTLAGGIAHDFNNVLAAIIGYAELVD
                     LDLPASSKVKPFLQRILRAAQRAKDLVAQILAFSRQEGEELRPVQAAPIVSEALKLMR
                     ASLPTTIEIRQSITTGPTVVLANPTQIHQVVMNLCTNAAHAMGKDGGTLEVCLERVDF
                     EEESALPCHDLTPGPFVVLCVRDTGHGMSPDILERIFDPYFTTKGKGLGTGLGLSVVH
                     EIVKRHSGAVTVESEVGKGSTFRVFLPGMDLETQEDAGEAPVAPLGSERILFVDDEPA
                     ITEVGAAMLRRQGYDVVTSTDSVRALELFRKDPRAFDLVITDMTMPQLTGDRLAEELM
                     KIRPGIPVILCTGYNESVSPHRIDSMGIRRLIHKPIGFQQLTQAVRSVLDDAPPFRDP
                     GAGRSPGKST"
     gene            3688..4089
                     /locus_tag="PLM79_16020"
     CDS             3688..4089
                     /locus_tag="PLM79_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOV87866.1"
                     /translation="MKRDPIGSVIAGLVLVLTLLWASATVGITDAPSQVSNIPVKGMV
                     TMVDLGSKECVPCKMMAPIIVELEKEYKNRAAIIFVDVWKQKDQSSQFEIRVIPTQIF
                     FDREGKEVYRHEGFLDKDSIVNMLRTLGVQG"
     gene            4106..4816
                     /locus_tag="PLM79_16025"
     CDS             4106..4816
                     /locus_tag="PLM79_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV87867.1"
                     /translation="MMDQLLLTVNSWMMTGTALAGLGCFLWGMISVLLSPCHMASIPL
                     IVSYVAGQDKGLRPRHAVHYALAFTVGLFITIAMVGIVCSLLGRMLGEVGPYWTVLVG
                     GILLWVAADMMGIAKCSMYGGLLGKIRIKGPAGAFVLGLAYGVLSGSCTFGFIAPILA
                     FITIQQKVATGILFIVLFGLGHSIPIAVAGSSTATVKNLLESSSFHEGGLWFRRCAGV
                     AIGVLGLYFVLRPFVGTT"
     gene            4843..5517
                     /locus_tag="PLM79_16030"
     CDS             4843..5517
                     /locus_tag="PLM79_16030"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HOV87868.1"
                     /translation="MSINDRPTVLACAGIQPELDFLLREGGAFEVRYLDQGLHQTPQK
                     MAGIVQEALDAVPLVVERVILGYGLCSKGILGLASRGRSLVIPRQHDCVSFYLQSFPE
                     ADGANLRTHTYYLTPGWLTLRKDPLTTVEEIYSPRVGREKAVWAMNRELQHYQCFAFV
                     DTGVCDPEPLRKRTMENAEFFGKEYREIRTALDYFRRLVTGPHSEPEFILLKPGEVLE
                     SSAWGL"
     gene            complement(5528..6076)
                     /locus_tag="PLM79_16035"
     CDS             complement(5528..6076)
                     /locus_tag="PLM79_16035"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOV87869.1"
                     /translation="MNYCSRCGTPLVYRTPSGDNRPRFVCETCRTVHYENPKLVVGSI
                     PEWQGRILLCRRSIDPRSGKWTLPAGFLENGETVSQGAAREALEEAGARLTDLVPFAM
                     FNIPYVNQVHLMFRAKLEDPHVWAGPESLEVGFFEEEGIPWNELAFPVVKETLKLFFL
                     DRDRGFRCFHLGDIPPISLDEF"
     gene            complement(6207..7076)
                     /locus_tag="PLM79_16040"
     CDS             complement(6207..7076)
                     /locus_tag="PLM79_16040"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HOV87870.1"
                     /translation="MAGNADIRVTSWVEVQQELFFESWNDGLQRFRSPYVFRGLSDAS
                     YGLKTSLLRLEGPFAKLEQHILRNFRKYAQGICGGFASFWHLLSVAQHHRLPTRLLDW
                     TYSPYVALHFATANLDKSDQDGVIWMVNFRKTHSLLPAPLQALLHDEGAEVFTVELLA
                     GLTPSRDTSGPSAVESSFHAVIQSLKDFDSLAGEEEFLLFMEPPSVDERIVNQFALFS
                     VMPNPERVVDDWLLRFPDLYRRVVIPAELKWELRDKLDQSNITERVLFPGLDGLSDWL
                     RRHYSPRRRGERG"
BASE COUNT         1532 a         2233 c         2143 g         1534 t
ORIGIN      
        1 gtaaaaagtc ctgaagagtg cgtttcttcg accctaaccg attgaattca ttaggttccg
       61 ttccgagaag aacgacttct tacgggatca tcaaggttct cttccgtgtt ggatggagga
      121 gggtgaaacc ctccccccgg acaaccctat ctcctttccc ctctcccttc ccccatcttg
      181 acactcccat gtcggaccga tacattgggt cactcaggaa tctttttctc ccggcagttc
      241 atggcggggg tttcggtttt tgtcctcatt gctttttgtt tgaacaaccc ggggatgggc
      301 aaggaagttc ctccatccgc agtggagtga ttccgatgat cccaggtcca cgcaatcttt
      361 cccccgctca cgggaatctg taggaaaccg atggcgcaac gagacccccg gcaggacggt
      421 ccccgggccg ggtccatccc ggggtttaca ggcgtcggcg gccggacgaa accaacaagt
      481 tgaccagggc cgatcctccc catgaagaac cgcggttatc acagacttcg tgtccacgtc
      541 atcaccctca tcctggtggt gcccgccttc gtcgtttttt acgaaatacg gaacctgacg
      601 gaagattacc atcaaacgat tcgttcggcc cagacccgcc tggccagcta tgccagcggg
      661 ctgtcggaac acgccacggt caccctctat tccgccgaaa ttgccctaaa atacgtggtg
      721 gatcgggttc aggagcacgg gctccttgaa ccccccgacg aatggaaaat ccacgagctt
      781 ctccgagacg cagcccgggg ttatccccaa tttggctctt tgctgatggt ggggtgcgac
      841 gggaagttgg tggcgaactc tctcgaattc cccgtgatcg aggttcaact ctcggatcgg
      901 gactatttcc ggtttcacct cgagcacgaa tccaccacgg agatccacat cagcaaaccc
      961 gtcatgaacc gtctcaacgg tctctggcgt tttttcatga cccgaaggct ttccaaaagg
     1021 gacgggtcct tcgggggcat cgccggggtg gcgttggatc ccgcgtacta cgaccgattt
     1081 taccagacca tcgatgtggg caagtcgggc cgaatccttc tcatccggat cgacggccgg
     1141 gtgctcatgc aaacgccttt ttcccccgag gccctcgaag tggattacgc acaacgtccc
     1201 atgcttgccg agcatcttcc ctcgtttccc tggggcacct accgaaatcc taccgcaggg
     1261 ttcgactcca ccgaccgcct catttcctac catcggctgg aaaaataccc cgtggtcgcc
     1321 acggtctcca tgctggtgga cgaaatcctg gccccttggc gggtgcgtct gagaaacggg
     1381 ttcatcaacc tggggatcct gctgttgctg gtggcgggac tcggctctct attgtggaac
     1441 cgattgaggg acctcgagaa ggttcaggca agtctcctgg aaaagcagga ggagctcagg
     1501 gaaagtcagc gtgccatgga caccctgttc aacaatcttc ccggaatggc ttatcgaagg
     1561 tcggccgacc cggactggac cctccggttt gccagtgaag gggcagggtc tctcacgggt
     1621 tatcgcccgg cggagcttac ccaaagtccc caagcctctt tcttcagcct gattcaccgg
     1681 gaagacgcaa tttccgcgcg caatgccgtc cagtccgcct tggcggacaa gcgatcctat
     1741 cgggttcagt atcggctgat atcccgggat cagcaggaac gatgggtatg ggatgcgggc
     1801 accggggtgt gggacgaggc cggccgggtg aaggccatgg agggcttcat caccgacgtg
     1861 agcgaactca gggcggtcga acaggctttg cgccgctcgg aggaaaaata ccgaaccctt
     1921 ttcgaggagg ccatcaacgg cattgtcctg gccgacgccc gaacggggat cgtcctcgac
     1981 tgcaaccgag cggcgctggc gatcttggaa cgctccaagg accaagtcgt cggaaaaccc
     2041 tggcagcctc tgagcccccg gcagtctcgg gatgatggaa ccgccagcga actgcaactc
     2101 aacccgggcc ctccgctgga caagatgatc gaaaccaagg tgatcacgcc ctccggcgcc
     2161 aacaaggaga ttgccctgaa agccagtcga gttctcgtcg agggccgcga acttgtgcag
     2221 tgtatctttc gtgacgtcac ggaagccaag aagctcgaaa gtcggttgca gcaagctcga
     2281 aagatggaag ccatcgggac ccttgccgga ggaattgccc atgacttcaa caacgttctt
     2341 gcagccatca tcggctatgc cgaactggtg gatctcgatc ttcccgcttc ctccaaagtg
     2401 aaaccttttt tgcagcgtat cttgcgcgct gcccagcgag ccaaggatct cgtggcacag
     2461 atcctggcct tcagtcgcca agagggtgag gaactccgcc cggtgcaggc ggcacccatc
     2521 gtttccgagg cgctcaagct catgagagcg tccctgccga ccaccatcga aattcggcaa
     2581 tccatcacca cgggtcccac ggttgttttg gccaacccca cccaaatcca ccaggtggtg
     2641 atgaatctgt gcaccaacgc cgcccatgcc atgggaaagg acggaggcac cttggaagtc
     2701 tgcctggagc gggtcgattt cgaggaggaa tcggcgctgc cctgccacga tctcaccccg
     2761 ggacccttcg tagtcctctg cgtgagggac accggccatg gaatgtcccc ggatatcttg
     2821 gagaggatct tcgaccccta cttcaccacc aagggaaaag gtctggggac gggcttgggg
     2881 ctctcggttg tccacgagat cgtcaagaga cactcgggag ccgtcaccgt ggagagcgag
     2941 gtggggaaag gcagtacatt ccgcgtgttt ttgcccggga tggatcttga gacccaggag
     3001 gacgccggag aagcgcccgt cgcccctctc gggtccgaga gaatcctgtt cgtcgacgat
     3061 gagccggcca tcaccgaggt gggtgccgcc atgctgagac gccagggata tgacgtggtg
     3121 acctccaccg acagtgtccg ggccctggaa ttgttccgaa aggacccccg ggccttcgac
     3181 ctggtcatta cggacatgac catgccccag ctgacggggg accggctggc cgaggaactg
     3241 atgaaaattc gacccgggat tcccgtgatc ttgtgcaccg ggtacaatga atccgtttcc
     3301 ccgcaccgga tcgactccat ggggatccgc cgcttgatcc acaaacctat cggtttccaa
     3361 caacttactc aggccgttcg atccgttctt gacgacgccc ccccgttccg ggaccccggg
     3421 gcaggtcgct ctcccgggaa atccacctag ggtttccaag ggctgatgat cccgtaagaa
     3481 gtcgttcttt tcggaacgga ccctaatgaa ttcaatcggt tagggtcgaa gaaacgcact
     3541 cttcaggact ttttgcgaaa ccgtcaaggg ctcattttta tgcactttcc acctcccgaa
     3601 ctctcgctaa aggcttgtaa ttttgtcgat tatcctccat atagaggtcg tttcccggac
     3661 gccactatca aaggaaagga gatcggggtg aaaagagatc caattggaag tgtcattgcg
     3721 gggttggttt tggttttgac acttttgtgg gcatccgcca cggtgggcat taccgacgcg
     3781 ccttcacagg tttcgaacat tccggtgaaa ggaatggtga ccatggtgga cctcggaagc
     3841 aaagaatgcg ttccctgcaa gatgatggcg ccaatcatag tggaattgga gaaagaatac
     3901 aaaaaccgcg cggccatcat ctttgtagac gtctggaaac agaaggatca gagctcacag
     3961 ttcgagatcc gggtgatccc cactcagatc ttctttgacc gggagggaaa agaagtctat
     4021 cgacacgaag gttttttgga caaggacagc atcgtcaata tgttgaggac ccttggagtg
     4081 cagggatgac ggggagggaa gaaggatgat ggatcaactt ctcttgacgg tcaactcctg
     4141 gatgatgacg ggaacggccc tggccggact gggttgcttt ctatggggga tgatcagcgt
     4201 gttgttgagt ccctgccata tggcctccat ccccctgatc gtgagctacg tggcggggca
     4261 ggacaagggc ctgcgccctc gacacgccgt tcattacgcc ctggccttca cggtgggact
     4321 gttcattacc atcgccatgg tgggaatcgt ctgctccctt ctgggaagaa tgctgggtga
     4381 ggtgggaccc tattggacgg tcctggtggg agggatcctt ctgtgggtgg cggcagacat
     4441 gatgggcatc gccaagtgtt ccatgtacgg cggtcttttg ggaaagatcc ggatcaaggg
     4501 ccctgcgggt gcctttgtgc tgggtctggc ctacggggtc ctgtcgggat cctgcacctt
     4561 cgggttcatc gcccccattc ttgccttcat caccattcag caaaaggtgg ccaccgggat
     4621 tcttttcatc gtcctgttcg gactgggaca cagcatcccc attgccgtgg ccgggagctc
     4681 cacggcaacc gtcaagaatc ttctcgaaag cagctctttc cacgagggag ggctctggtt
     4741 caggcgatgc gccggtgtgg ccatcggcgt cctggggctc tatttcgttc tccgcccctt
     4801 tgtgggaacc acctagcctc ctgccgcgga gccctcgatt ccatgtccat caacgatcga
     4861 cccaccgtcc tcgcttgtgc cggcattcag ccggaactgg atttcctcct ccgggagggg
     4921 ggcgccttcg aggttcgtta cctggaccag ggtctgcacc aaacccccca aaagatggcc
     4981 gggatcgttc aagaagcgct ggacgccgtg cctctcgtcg tcgaaagggt aattctgggc
     5041 tacggactct gttccaaggg catcctgggg cttgcatccc ggggccggag cctcgtcatt
     5101 cccaggcaac acgattgcgt ttccttttac ctgcaatcct tccccgaagc cgatggggcc
     5161 aatctgcgaa cgcacaccta ttatcttacc cccggttggc ttaccctgcg aaaagacccc
     5221 ctcaccaccg tcgaggaaat ctactcaccc cgggtgggca gggagaaggc cgtgtgggcc
     5281 atgaaccggg agcttcagca ttatcagtgc tttgcctttg tggacaccgg ggtctgcgac
     5341 cccgagccct tgcgcaaacg aaccatggaa aatgccgagt ttttcgggaa agagtaccga
     5401 gagatccgga cggccctgga ctactttcgg cgactggtta ccggccctca ctcggagccc
     5461 gaattcatcc tgctgaaacc gggggaggtt ctcgaatcat ccgcctgggg tctttaggca
     5521 ggttccatca gaattcatcc aaggagatag gcgggatgtc cccgaggtga aaacagcgaa
     5581 accctcgatc ccgatccagg aaaaacagct ttagggtttc cttcaccacc ggaaatgcca
     5641 gttcgttcca tggaatgcct tcttcctcga aaaaccccac ctccagactc tccggtcctg
     5701 cccacacatg ggggtcctcg agtttcgcgc gaaacatcag gtgaacctga ttcacatatg
     5761 ggatgttgaa catggcaaac gggacgaggt ccgtaagccg ggcccccgcc tcttccagtg
     5821 cctcccgagc cgccccctgg gacaccgtct ccccgttttc caggaatccg gcgggcagcg
     5881 tccatttccc ggacctggga tcgatggaac ggcgacacag gaggattctg ccctgccact
     5941 cggggatgga gcccaccacc aacttggggt tttcataatg gacggtgcgg catgtttcgc
     6001 agacaaaacg gggacgattg tcccccgacg gggttcggta aaccagcggc gttccacacc
     6061 ggctgcaata gttcatgacc gacctcctgc tgtgcctcgc ccggttcgcg tccattcagc
     6121 ctgcagaccg tgaccgggaa aaaagggggc tgccatcctt ccctccccag ggggccgggc
     6181 ggcgagtccc gccccctccc ccgctctcac ccccgctccc ctcgcctcct ggggctgtaa
     6241 tgcctgcgca gccaatcgct caggccgtcc aggccgggga agagcacccg ttcggtgatg
     6301 ttggattgat ccagcttgtc ccggagttcc catttgagct cggcaggtat caccactcgc
     6361 cggtataggt ccggaaacct gagcaaccag tcgtccacca cccgctcggg attgggcata
     6421 acggaaaaga gggcaaactg gttgacaatg cgctcatcga cggaaggggg ttccatgaat
     6481 aggaggaatt cttcctcccc cgccagcgaa tcgaagtcct tgagtgattg aatgaccgca
     6541 tgaaaggagg attcgaccgc cgagggcccg gaggtgtccc gggaaggggt caagcccgcc
     6601 aggagctcca ccgtgaagac ttcggctccc tcgtcgtgga gcaatgcctg caggggagcg
     6661 ggcagcaagc tgtgagtttt gcgaaaattc accatccaga tgaccccgtc ctgatcggac
     6721 ttgtccaggt tcgccgtggc aaagtgaagg gccacgtaag gcgaataggt ccagtccagg
     6781 agtcgggtgg gcagccggtg gtgttgagca accgagagca aatgccagaa ggaagcaaag
     6841 ccgccgcaga ttccctgggc gtactttcga aaattgcgca gaatgtgctg ctccagcttt
     6901 gcgaaaggcc cttccaaccg cagcagggaa gttttcagtc cgtaggacgc atcggagagc
     6961 cctcggaaga cgtagggcga ccgaaaccgc tgcaggccgt cattccagga ctcaaaaaag
     7021 agctcttgct ggacctccac ccaggaggtc acccggatat ccgcatttcc agccaacgaa
     7081 attccctccg tggcgacatc ccttcaaacc ggccccggca ccttcaggag gtccgaatcc
     7141 cggatcaagc ctgcgtccct tgctgggaga gtctcgagag taggccttcc ccttcgccgg
     7201 cctgtgaggt gaccgaggag cagacacatc aaatgtgcga ccttccagac ccctttgggc
     7261 gcactccctt cgaaaagaag ggcattccgc tgagcgtaga atcaaggccc aggcaagccc
     7321 accgagattg ccccactccg gggtgacacg gacccccccg tggaatcatc gccaactcct
     7381 tcgggcaggg ggggtgtgtt gggaacttgg gttttagcat acccccaagc ggggctggtt
     7441 tc
//
LOCUS       DAOOVC010000181         7429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5981_length_7429_cov_5.773393, whole genome shotgun sequence.
ACCESSION   DAOOVC010000181 DAOOVC010000000
VERSION     DAOOVC010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5981_length_7429_cov_5.773393"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2299)
                     /locus_tag="PLM79_16045"
     CDS             complement(<1..2299)
                     /locus_tag="PLM79_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOV87871.1"
                     /translation="MKLLLQCIHAWTAWALSRPLLVFGLSAVSAILFFVLSLTHLDME
                     TDQLELISTNHPLIALQEKLDPFNFEDQTTFTVVVEAPTPREAIDYVKAMVPRIREDR
                     KNFRDVFYRIEPRQFRQWAFLYLEKDDLLKIRDALAENAPLIDGLARDPEVLAFFRLI
                     NREMSTRMVGELFTGFLDNPSPTEGSDGGGKPVDLAFLIQALEGLSGFLKGASPYVSP
                     WGNFFHNGYRDLEQEGYFWEGDKRYLVFFVVPQKEEERFNNTQTAMDQLRRMIREARS
                     EFPDVQAGVTGQEALNNDEMETALGDMNRATWFSLAGVWALMLVFLRSVRRSVAKMVS
                     VCIGLAWTFGFAALSIGHLNILSLVFAPLLIGLGDDFGVHWYSRYEEEERNGSGNVGE
                     SIQRVMDRSGPAIVVAGLGISLSFLPFVLTGFRGLVELGIIAGVGILLILLATFVVLP
                     TLTPILGGRLRPGTNSAPQEPGPRDLIRFSPTTARWVLLGVAAVSVLALVSGRRVGFD
                     LNPLRLQSVRAESVIWEKRLVEGAQRSILSAAVFAHSPQEVLEKSRALEKLPSVSEVE
                     NVFSFLPLNQEEKVPLVRSVLADFPEIQTGQGTDDPADIPALVEVMERIRFKMRDENA
                     AEWGAEDATLQQLARARSLTGQIIAELRTGNPEVLDRLKAYRGLFTRDLADQWQLLKE
                     TASVSPMEVKDLPRELRSKFLRDDLYVIRVYPRESVWDEGALGRFVQDLQSVDRQAVG
                     DPVSLYVFAQAFKKACIDASLYAVLA"
     gene            3301..3960
                     /locus_tag="PLM79_16050"
     CDS             3301..3960
                     /locus_tag="PLM79_16050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOV87872.1"
                     /translation="MNSRIPLRTYSPGEEIANSITHGVGVVFSIAGLAVLTAFASVFG
                     TAWHVVGCSIYGTTQILLYTASTLYHSVPQPRAKAVLRVLDHGAIFLLIAGTYTPFTL
                     VNLRGPWGWSLFGLVWGLAVLGIALQGVLIRRRAVFAVVPYVAMGWLVVVAAKPLLES
                     VALGGLILLLAGGLAYTLGSLFYTWHRLPYHHAVWHVFVLAGSVLHFFAVLFYVIPLA
                     A"
     gene            complement(3979..5265)
                     /locus_tag="PLM79_16055"
     CDS             complement(3979..5265)
                     /locus_tag="PLM79_16055"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOV87873.1"
                     /translation="MNPRSPNASDEDFALLGLKPGASPAQIKQAYRELAKKWHPDYFQ
                     QERPGDRRRAEERFKDITAAYRRLSRQWKAFSEKTETDVSGGSGAKRRTRATPGGSAT
                     RAGIWGSWPARFLRSTGPSGQEPPAVKTSRAIRVGIGCILLAACAAWVISRWWPMESR
                     VLRLTDLGPPPVEAPSPPPAHPFPSRGHGAEPVPVPPFLPEAVPDTAAPAAESGPLPP
                     ESSSSREEPPPFFTLGSSREEVLRVQGPPTRIHGQTWVYNLSEVHFKEGHVSRYDNFD
                     GSLKVHLPPSAHPPGQPPHFFSLGSSRNEVLTVQGTPTRVHGNRWYYGFSEVRFRNDH
                     VEGYDNYFGNLYVRLLPSDPLPEGHRRESFTVGASADEVLAVQGTPTSIQGNTWFYEL
                     SSVLFREGKVQYVNNVGGNLRYVPPAENSWKEKGSG"
     gene            5437..6297
                     /locus_tag="PLM79_16060"
     CDS             5437..6297
                     /locus_tag="PLM79_16060"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOV87874.1"
                     /translation="MSKIELSSPLRDAVGAAQKLPPFPEVVWKVMPLVERMAPVAEIE
                     AVIRFDPVITAKVLVLSRSPSFSRGQKITSLRDAIVLLGQQRLVQVVTAACSSTYFMG
                     EAPGYDLREGELWEHSIATALMTEIVAEHLGWPEKTTAFTAAILHDIGKTILHHFVGA
                     YFESIMALVGEKKKGFLDAEREVLGIDHQQLGERIALRWRLPSPVVTAIGFHHQPRQA
                     LVHQQLVSLVYLCDRMASAMGIGCGVDGLMQPNEDDVCIEMGITPRMIERFCADLVDR
                     LGETRRFLSS"
     gene            6477..6737
                     /gene="csrA"
                     /locus_tag="PLM79_16065"
     CDS             6477..6737
                     /gene="csrA"
                     /locus_tag="PLM79_16065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HOV87875.1"
                     /translation="MLVLTRRVGESIRICDDIEVVVTAIDQNKVKIGIRSPRHIPVYR
                     EELYQKIQEDNLRAARMKTGDLDDMLAMLEKEGNPETPLGES"
     gene            6761..7207
                     /gene="fliW"
                     /locus_tag="PLM79_16070"
     CDS             6761..7207
                     /gene="fliW"
                     /locus_tag="PLM79_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOV87876.1"
                     /translation="MRIETTRFGPLELDESTLIRFPWGIPGFETLKRYVLLEHGKGPF
                     QWLQAVDEPAVAFVVCAPEALGFQYKIPREKGETIRLDNWDDLVVLVMVSFDRNHAVI
                     RPHQCGPLLFNSLLKIGYQWTIDARELSRYISGADPAARPSPPSSE"
BASE COUNT         1450 a         2213 c         2309 g         1457 t
ORIGIN      
        1 tggccagcac ggcatacagg gacgcatcga tgcaggcctt cttgaaggcc tgggcaaaga
       61 cgtacaggga gacgggatcg cccaccgcct gccggtccac gctctgcaaa tcctgaacaa
      121 accgtcccaa ggctccttcg tcccacacgg attccctggg gtagacgcgg atcacgtaaa
      181 gatcgtcgcg gagaaacttg gatcgcagct cccggggaag gtccttgacc tccatagggg
      241 aaaccgaagc cgtttccttg agcagttgcc actggtccgc caggtcccgg gtgaagaggc
      301 cgcgataggc tttcagtcga tcgaggacct ccgggttgcc ggttcgcagc tccgcgatga
      361 tttggcccgt gaggctccga gcccgggcca actgctggag agtcgcatcc tcggcccccc
      421 attccgccgc gttttcgtcc cgcatcttga agcgaattcg ctccatgact tccaccaggg
      481 ccgggatgtc tgcgggatcg tccgtccctt gcccggtttg aatttcggga aaatccgcca
      541 ggacggagcg gaccaggggg accttttctt cctggttcag ggggagaaaa ctgaaaacat
      601 tctcgacttc ggagaccgag ggaagcttct ccaatgcccg ggacttctcc agtacctcct
      661 ggggagaatg ggcaaagacg gccgccgaaa gaatggatct ctgggcccct tccacgaggc
      721 gcttctccca gatcacggat tccgcacgga cggactggag acgcagggga ttgaggtcaa
      781 aacccacccg gcggccgctc accagggcaa ggacgctgac cgccgccacc cccagcagga
      841 cccaacgggc cgttgtggga gaaaaccgaa tcaggtccct gggacccggt tcttgggggg
      901 cggaattcgt cccgggccgc aaacgcccgc ccaggatggg ggtcagggtt ggaagcacca
      961 cgaaggtcgc cagaaggatg agcagaattc ccaccccggc gatgattccc agttccacca
     1021 ggcctcgaaa gcccgtcagg acgaaaggca ggaaagacag ggatatgccc aatcccgcca
     1081 ccacgatcgc agggcctgac cggtccatga cccgctggat gctctccccg acgtttccgc
     1141 tcccgtttct ttcttcctcc tcgtagcggc tgtaccagtg aacgccgaag tcgtccccca
     1201 gcccgatcag gagaggtgca aacaccaggg acaagatatt gaggtgcccg atggaaaggg
     1261 ccgcaaaccc gaaggtccag gcaagtccga tacacaccga gaccatcttc gccacggacc
     1321 ggcgcacgct gcgcaggaac accagcatca gggcccagac gcccgccagg gaaaaccagg
     1381 tcgcccggtt catgtccccc agggcggtct ccatctcgtc gttgttgagg gcttcctggc
     1441 ccgtgacccc cgcctgaacg tcgggaaact ccgaacgggc ctctcgaatc atccgacgca
     1501 gctgatccat ggccgtctgg gtgttgttga accgttcttc ctccttttgg ggcaccacga
     1561 agaataccag gtaacgcttg tcgccctccc agaaatagcc ctcctgttcc aggtcccggt
     1621 atccgttgtg gaaaaagttt ccccaggggg aaacgtaggg ggaggcgccc ttgaggaagc
     1681 ccgacagtcc ttccagtgcc tgaatgagaa aggccaggtc cacgggcttg ccgcctccgt
     1741 cggagccttc cgtgggggaa ggattgtcca gaaaccccgt gaagagttcg cccaccatgc
     1801 gggtgctcat ctcccggttg atgagccgga agaaggccag gacctcgggg tcccgggcca
     1861 ggccgtcgat gagaggggcg ttttccgcca gggcgtcgcg aatctttagc aggtcgtctt
     1921 tttcgagata tagaaacgcc cactgccgaa attgtcgagg ttcgatgcgg taaaaaacat
     1981 cccggaaatt cttccggtcc tcacggatgc gggggaccat cgccttgacg tagtcgatgg
     2041 cctcccgggg cgtaggcgcc tccaccacca ccgtgaaggt ggtctggtct tcgaagttga
     2101 aaggatccag cttttcctgg agggcgatta ggggatggtt ggtggaaatg agctcgagct
     2161 ggtccgtctc catgtcgagg tgggtgaggg agaggacaaa gaacaggatg gccgagacgg
     2221 cggaaagtcc aaaaacaaga agcggtcggg acaatgccca ggccgtccat gcgtgaatgc
     2281 attgcagcag cagtttcatt cgaggatctg ttgtgtcctg aaggtatggc tcacggagaa
     2341 aagaccgacc gataagcgcc gggcgtcccc gttaccggct tcccgggttg ctcgtcctcg
     2401 agccgtagcc cgacgtgttt ccggccgttg gtcggcgcgg ccccgcggga aggcccgata
     2461 tgagggcttc cgtgcactca ccgaaacgcg acctgcatct tcttgaaata gttaagggag
     2521 cggacgatgc tcccgtttcg gcggtcttca aataacaact tctcccccga cggtcaacaa
     2581 gatctttgga gtcgacaccc ggtcccacct ccccggaccg gcccgtcctc agcgaaccgg
     2641 ggtgcttgcg gccttcattc ggcggtattc ctccaccgtg agatccacgt cggccgcctc
     2701 aggcatggag catgggaaat ccccaggcgc cggtcccata agagggacct cgtgaggacg
     2761 acaagtgtgt tctggaccga acggcattcc ttggtacccg ccacccttaa ggcagtcggg
     2821 gacccaccgg agggggaaaa gatgatattt ccttttttca caagactcgt tggaacttat
     2881 aggaagcgtt ctccgaccgg atcctttggg ggtccggaag gcaaagaagg tctccgtgca
     2941 tttgagggac ccaaagggtc gacaagatcg acggcaagac cgttttgatt tcgtcgtaga
     3001 cctgtccatc catctcgaca aggccttcgg cgtcatcccc gcggccatcc ccggagaaga
     3061 aaagatcaag aagtctatcc acttacggga accgcccgat ccctgccccc ggcgtcccgt
     3121 gcgccgggga cgctcgtttc cttgacggag tcctgtatgg tgggctaaga agcgacatct
     3181 cggccggatc gccgggggtc gggggggctg gtggaggtcc acgcaccggc aaacaggaga
     3241 tggggagaat ccggatcaat tcgttgcagg tttgccgttt cggtgcggtt tggaaggcgt
     3301 atgaactcga gaatcccgtt gcgcacttac agtccggggg aagaaatcgc caacagcatc
     3361 acccatggcg tgggggtcgt attttccatc gcgggtctgg ccgtgctcac ggctttcgcc
     3421 agcgttttcg gaactgcttg gcacgtggtc gggtgcagca tttacggcac cacccaaatc
     3481 ctcctctaca cggcgtccac tctctaccac agtgtcccac agccccgggc caaagccgtt
     3541 ctgcgcgtcc tggaccacgg cgccatcttc ttgctcatcg cgggcaccta cacccccttc
     3601 accctggtga acctgcgcgg tccctggggt tggtccctct tcgggcttgt ttgggggctg
     3661 gccgtcctgg gcatcgccct gcagggtgtc ctgattcgtc gccgggcggt gttcgccgtt
     3721 gtgccctatg tggccatggg ctggctcgtc gtcgttgccg ccaagcccct cctggaatcc
     3781 gtggccttgg gagggctgat cctgctcctg gcgggaggcc tggcgtacac tttgggcagt
     3841 ctcttctata cgtggcaccg tttgccctac caccacgccg tctggcacgt ctttgtactg
     3901 gccggaagcg tgctgcactt cttcgcggtt ctcttctacg tcatccccct ggccgcttaa
     3961 actccgctcg gcgaccggtc agcccgatcc tttttccttc caagaatttt ccgcaggagg
     4021 gacgtagcgg aggttcccgc cgacgttgtt gacgtattgg accttgccct cccggaaaag
     4081 cacgctggac agttcgtaga accaggtgtt tccctggatg ctggtggggg tgccctggac
     4141 ggcgaggacc tcgtcggcgc tggccccgac ggtgaacgat tcccggcgat gtccttccgg
     4201 gaggggatcc gatggcagaa gacgcacgta gaggttcccg aagtagttgt cgtatccttc
     4261 cacgtgatca ttcctgaacc tcacctcact gaagccgtag taccatcgat tcccgtggac
     4321 acgagtcggg gtcccctgaa cggtgagcac ctcgttgcgc gaggacccca gagagaagaa
     4381 gtgaggaggt tggcccgggg ggtgggcgga agggggcagg tgcactttga gggacccgtc
     4441 gaagttgtcg tagcgagaga catgaccttc cttgaaatgc acttccgaca ggttgtacac
     4501 ccaggtctga ccgtgaattc tggtaggcgg tccctggacc ctgaggacct cttcccggga
     4561 agatcccagg gtgaagaacg gcgggggctc ttcccgggaa gaggaagact cgggggggag
     4621 aggcccggat tccgcagcgg gggccgccgt gtccggaacg gcttcgggca ggaaaggagg
     4681 cacgggaacg ggttccgcac cgtggcctcg agacgggaac gggtgcgccg ggggagggga
     4741 aggggcttcc acgggaggcg ggcccaggtc ggtgaggcgc aagacgcggc tttccatggg
     4801 ccaccaacgg gaaatcaccc aggcggcgca agccgccaaa aggatgcagc cgatgccgac
     4861 ccggattgcc cgggaggttt tgactgccgg cggctcttgg ccggaggggc ctgtggatcg
     4921 gagaaaccgg gccggccaac ttccccaaat tcccgcccgg gtggcggatc cgccgggagt
     4981 cgcccgggtc cgcctcttcg ccccgctccc ccccgaaacg tccgtctccg ttttctcgga
     5041 gaaagctttc cattgccggg aaagccgccg ataggcagcc gtaatgtctt tgaatcgttc
     5101 ttcggccctt cgcctatccc cggggcgctc ttgctgaaag taatccgggt gccatttctt
     5161 ggccaattcc cggtaggctt gcttgatttg agcgggagaa gctccgggtt tcaagcccaa
     5221 aagggcgaaa tcctcgtcgg aagcgttggg ggatcgaggg ttcatgggcg cgaccgttcc
     5281 gggccttgtg gccgacttcg taccgggact ttcccgggaa agactcctcg gcgaggatag
     5341 cggctgggga aaaaatattc aagttattcg gccggctttt cgataggctt gctgtatgga
     5401 agcgaacgcg gtttgtcctt caggcaggat tgaacaatga gcaagatcga gctgtccagc
     5461 cctctccggg atgccgttgg cgcggcgcag aaattgcctc cctttccgga agtcgtatgg
     5521 aaggtcatgc cattggtgga acgaatggct cccgtggccg aaatcgaagc ggtgatccgc
     5581 ttcgatcccg ttattacggc caaggtcctg gtccttagta ggagtcccag tttcagccgg
     5641 ggacaaaaga tcacctccct gagagacgcc attgttctcc tgggacaaca acggttggtc
     5701 caggtcgtga cggccgcctg ttcctcgact tatttcatgg gggaagcgcc ggggtacgac
     5761 ctgcgggaag gagagctctg ggaacattcc atcgccacgg ccctcatgac ggaaatcgtg
     5821 gcggaacacc tggggtggcc ggagaaaacg acggccttta cggcagccat ccttcacgac
     5881 attggaaaga ccattctcca tcactttgtg ggcgcgtatt tcgaatccat catggccctg
     5941 gtcggggaaa agaagaaagg ctttctggat gcggaaaggg aggttctggg aatcgaccac
     6001 caacaactgg gagaacggat tgccctgcgt tggagacttc cttcccccgt tgtcacggcc
     6061 attgggtttc accaccagcc tcgacaggcc cttgttcacc agcagctggt gtccctcgta
     6121 tacctttgcg accggatggc ttccgccatg ggaattggat gcggggtgga cggcctcatg
     6181 cagcccaacg aagacgacgt atgcatcgag atgggaatca ccccgcggat gatcgagcgc
     6241 ttttgcgccg acctcgtgga tcggctggga gagaccagga gatttctgtc ctcctgaaga
     6301 acgaggtttc ttcaggaagc ctcgacgtac cggcgagtcc gggaatcgga cctcctgcct
     6361 cccggggacg accctcgtcg gaaagccctt tcgcagttgc tccggatttg gtagcatgac
     6421 tcctctagcc gggtctcagt cctaggaacg ggatgcctcg atgcgggtga gggtctatgc
     6481 tggtgctcac aagaagagtg ggggagtcca tccggatctg tgacgacatc gaagtggttg
     6541 tcacggccat tgaccagaac aaggtcaaga tcggtatccg cagtcctcgt catattcccg
     6601 tctaccggga agagttgtat caaaagatac aggaagacaa tctgcgggcc gctcgcatga
     6661 aaaccgggga ccttgacgac atgctggcaa tgctggagaa agaaggaaat ccggagaccc
     6721 ccctggggga atcttgaccc tctcattcga ggcggattct atgagaattg aaaccacccg
     6781 gttcggaccc ctcgagttgg atgagtcgac cctcatccgg tttccgtggg ggattccggg
     6841 attcgagacc ctaaaaagat atgtcctcct ggaacacgga aaaggtcctt tccagtggct
     6901 ccaggccgtg gacgaacccg ccgtggcttt cgtggtctgt gcccccgaag ccctgggatt
     6961 ccaatacaag attccccggg aaaagggcga aaccatacga ctggacaact gggacgacct
     7021 ggtggtcctg gtcatggttt ccttcgaccg gaatcatgcc gtcattcgac cccaccaatg
     7081 cggccctctg ttgttcaact ccctgttgaa gatcggttac caatggacca tcgacgcccg
     7141 ggagctttcc cgctacatta gcggagccga ccccgccgcc cggccttccc cgccttcttc
     7201 cgagtgaggt ccgaccggag agatttccaa cgcaacacga cggccgggtc ctgaagggcg
     7261 gtcgtttacg ggtctcggtt gggtccgggg tcttggtttt taaggggtgg aacaggaaag
     7321 gcgatcgaac cccttcgggt ccggtaggat gaaaaggccg actcgaaggt acccaccgaa
     7381 acgcggcgtt ccagcacggc ggatgcccga cgggccgttg cccccctgg
//
LOCUS       DAOOVC010000182         7421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_5989_length_7421_cov_5.189519, whole genome shotgun sequence.
ACCESSION   DAOOVC010000182 DAOOVC010000000
VERSION     DAOOVC010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7421
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5989_length_7421_cov_5.189519"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..428
                     /locus_tag="PLM79_16075"
     CDS             279..428
                     /locus_tag="PLM79_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87877.1"
                     /translation="MDSAGKKDCKWEGRDFTHGTEVCDSERCMRCNDGDWEERFQDLI
                     YGYGP"
     gene            complement(962..2206)
                     /locus_tag="PLM79_16080"
     CDS             complement(962..2206)
                     /locus_tag="PLM79_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOV87878.1"
                     /translation="MTAEAKATMPRWIPLLLGLLGSTTCGMLLYAWSVFIKPLNAEFG
                     WTRAEIAMAFAICCLVFGLVTFPAGRWSDKIGPRKVVMSGGVLLAIGFILSGFIQSKV
                     QLYITYGIIAGLGGGMIYLPPIATAPKWWPDRRALATGFAVVGLGLGSFLMGPLATYI
                     IEKPGFGWRYVFYYCGIAMGVMALIAGAFLEPPPAGWKPAGWAPPAPPAGAAAPKVTR
                     DWTHEEAKKDAKFWLLYLAYFCGSFAGLMVIGHLAGFGRDQGLTAMAAAGAVSSLAFA
                     NAATRILSGLFVDWIGIRVYFAATFALQTLAMIAIFQLGGSFYGLAAIAIIIGWDYGA
                     MFTLFPATCLQFYGPTAQGSNYGLLFTAWGLAGFAGPWVGGWLKDATGTYYIPFLCSA
                     ALCALGTVIIITTKPPEKKHAD"
     gene            complement(2654..3070)
                     /locus_tag="PLM79_16085"
     CDS             complement(2654..3070)
                     /locus_tag="PLM79_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87879.1"
                     /translation="MEEKISELLRKWKGLLEGAWKEMPRHFRNDHTVRRTLQCFWFGR
                     LKDMGFRVVADYLPPRISDRPIDLIALNEEDEIVYAVCFDTVVTLAAVKSLSSFESQN
                     RIIFTIGALEKKVQESRFFLKGDIQHCHLASPGTRS"
     gene            3598..5028
                     /gene="rfaE1"
                     /locus_tag="PLM79_16090"
     CDS             3598..5028
                     /gene="rfaE1"
                     /locus_tag="PLM79_16090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOV87880.1"
                     /translation="MPPSILILGDVMLDQYVWGEVRRVSPEAPVPVVRVCTRTHGLGG
                     AGNVAANLAGLKADAFLLGVCGDDSAGVRLRALIEANGIRGSLSVDPSRKTTTKTRVM
                     AQGQQLMRLDEEDSIPVSPSLEDALLDAVREQLPGCGAVILSDYGKGILTSLGFVRQL
                     IESARTSGVPVLVDPKGTDWERYRGAACITPNLSELQAVAQPFPAEDDAQVAGAAGKV
                     LEKFGLDRILVTRGARGMCLLEKDSKPHFIRSAARQVFDVSGAGDTVIATLAYGIARG
                     DSFEQSARRANLAAGIVVGKLGTQPILLPELEAAVQAEETGSVSPYPRKLASLEDARA
                     RILQWRSTGAVIVFTNGCFDLLHPGHIHLLKQARSLGDRLIIGLNSDESVRRLKGPAR
                     PILSEHDRAAVLGALEAVDLIVVFGEDTPLELISALRPDILVKGSDYTLDTVVGRDLV
                     ESYGGRVRLVPLLDGHSTTRLVRMVG"
     gene            5127..5507
                     /locus_tag="PLM79_16095"
     CDS             5127..5507
                     /locus_tag="PLM79_16095"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOV87881.1"
                     /translation="MGSTKRFEDLECRREARNFVGMVDGLVSKDRFRKDYDLASQVKR
                     SAVSAMANIAEGFHRRGNREFIKFLDCSRSSLAETLSRLHAASDLQYIEEAEMNTLRR
                     QTDLVRKKVNNLMTYLHQTNRPTN"
     gene            5656..7257
                     /locus_tag="PLM79_16100"
     CDS             5656..7257
                     /locus_tag="PLM79_16100"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOV87882.1"
                     /translation="MPGPEDYEAELKRLQKLVAEQRLLGREIVVVMGVGFVGAVMAGV
                     VADSVDRSTGRPNKFVIGMQRPSVRSYWKIPYLNRGIVPVQAEDPEVAPLIRRCVLEK
                     KTLTATFTYDVLSLADVVVVDVQCDYLKETLGNVRQGKADIGALEESLRVLGERIQPD
                     CLVLIETTVPPGTTEYVAYPILKRAFERRGLNDSEPLLAHSFERVMPGRNYVASIRDF
                     WRVCSGINETARKRVTAFLSDVLNVDRYPLTVLDRPIESETCKIVENSYRATILAFLH
                     EWSLFAERNGVDLIKVIEAIKVRPTHSNMIFPGPGIGGYCLPKDGGLGVWAYHTLMGF
                     EDNIFKITPLAIDINDTRALHVTQLVRDALRNMGKIVAASKIALLGASYREDVGDTRY
                     SGSELIVRKLVEMGGDVSVYDPYVRRWWELEKQDTYPAPGLSWARFFRNQERLRELRI
                     ADTMARALQDADALVLAVRHKDFLELDPEEVVEHARGPLAVVDCFGILHDEKIRRFFE
                     LGCEVKGLGRGHIQRIKETVKKGRR"
BASE COUNT         1596 a         2200 c         2219 g         1406 t
ORIGIN      
        1 ctaccgtata atgttgtgtc gactgagttt acgtcccacc aagacccatg accttgagcc
       61 gtttgtgcca tagatcccca cccctgtcaa taattgtgcc aaaaagaact tgggaactcg
      121 gaagcggaag ggcgaggggc gaaaggttac caaccagcca aaccgtcggc cctccaaatg
      181 attatcattc ttgtgtaggt cggtcggcca aagaaacacc ggccttcgat catgaatcaa
      241 agcgggcagc aaaacgccgg cagaaaagga gactttccat ggattccgct ggaaaaaagg
      301 actgtaaatg ggagggccgc gactttactc acgggaccga agtctgcgac tcggagcgtt
      361 gcatgcggtg taacgatgga gactgggaag agagattcca ggacctcatc tacgggtacg
      421 ggccctaatt ctccccctct ctcagggacc gaggccttga aagagcgcag gatgacgcgt
      481 tttttccggg tccggcaaga gagggaaaga tggctgcacc gaaggtcccg ctcaaacccc
      541 tctgtcagcc ggggggatgg agctctttct ccggtgatgg aggggagacg tctttggggg
      601 gaggagcctg ttcacggagg aggcctttgg cggaaattca gggcgcgggg tgatttgtcc
      661 cgtggccccg gaggagggcg gagagagaag gaagggaccg acacggtttg cccgtgctgt
      721 tttgacggca agatgtgacg gaggaaaacc ctgcacaaac acaaaccccc gagacctcgt
      781 ctcgggggtt tgtttgcttt tcatcccggc ggcccttgca cccgaacccg acgggtgggt
      841 tttcatccac cctacaccga tcctttcagt gttccaagag tttgcggctc agggtcgtcg
      901 ccgccaagac gacggagaaa ggaccgggag aatccggcaa ggatcccggc cggggtattc
      961 cttaatccgc gtgtttcttt tcgggcggct tggtcgtgat gatgatgacg gttcccaagg
     1021 cgcacagggc cgcggagcac aggaacggaa tgtagtaggt ccccgtggcg tccttcagcc
     1081 agccgccgac ccaggggccg gcaaatcccg ccagacccca tgccgtaaac aggagcccgt
     1141 agttgctccc ctgcgccgtc ggcccgtaga actggagaca cgtggccggg aatagggtaa
     1201 acatggcgcc gtagtcccac ccaatgatga tggcgatggc cgccaacccg tagaagctgc
     1261 cgcccaactg aaagatggcg atcatggcca gggtctgcag ggcgaaggtc gcggcgaagt
     1321 aaacgcgaat gccgatccag tccacgaaca accccgagag gattctcgtg gctgcattgg
     1381 cgaaggccag ggagctaacg gccccggccg ccgccatggc cgtcaggccc tggtctcgac
     1441 cgaaaccggc caggtgtccg atgaccatca agcccgcaaa agaaccgcag aagtaggcca
     1501 ggtacaacag ccagaacttg gcgtcttttt tggcctcctc gtgggtccag tcacgggtta
     1561 ccttgggagc ggcggccccg gcggggggag cggggggcgc ccaaccggcc ggcttccagc
     1621 ccgcaggggg cggttccagg aacgcaccgg ctatgagggc catgactccc atggcgatgc
     1681 cgcagtagta aaacacgtaa cgccatccaa aaccgggttt ttcaatgata taggtagcca
     1741 acggccccat gagaaaggag ccgagaccca ggcctaccac cgcgaagccg gtagccaggg
     1801 cccgtcgatc cggccaccac ttgggagccg ttgcaatggg gggcaggtag atcataccgc
     1861 cgcccaagcc tgcaatgatc ccgtaggtga tatacaactg gacctttgat tggataaagc
     1921 ccgataaaat gaatccaata gccaaaagga caccacccga catcaccact tttcgaggac
     1981 cgatcttgtc actccaacgt cctgcaggga acgtaaccaa cccgaacacc agacaacaga
     2041 tcgcaaacgc catggcgatc tcggcgcgtg tccacccgaa ctccgcgttc aacggtttga
     2101 tgaagacact ccacgcgtat aaaagcatgc cgcacgtggt gctacccagt aagcccagca
     2161 acaaagggat ccatcttggc atcgttgctt ttgcttctgc tgtcatagtt tgagccctcc
     2221 tttcaaagat caataaagaa tggtgtgcga taagtgcgcc gtgacaatcc gataggttac
     2281 ttttggaaaa gacgatctgg acaccactag aagaggaaca tcagcacggc ggtgacaaca
     2341 ccagggaaaa ccaaagccca caacaaaatg tcactaccga gagagcagga aacatggaaa
     2401 cgaataagac agaaaagctt gaaacctaag aacaatgaca caccagtccc ccttcacctc
     2461 ctttcgacgg catcggttta tgccgagctg tttgtttttc cttctgcaat ccccttaagc
     2521 ccggtatgaa gaaatatgcg catttttgca caatagcaga cccatgtcaa ggcagatcga
     2581 gacccaacaa agatttagcc gctgtcggca ggcagctcct ccttggaccg gggatggttg
     2641 actcaacgga aggtcaggac ctggttcccg gggaggccag gtggcagtgt tggatatcgc
     2701 ctttgaggaa aaagcggctc tcctgcacct tcttctccag ggccccaatg gtgaagatga
     2761 tccggttttg ggactcgaag ctgctgaggc ttttcacggc ggcaagggtc accacggtgt
     2821 cgaagcacac ggcgtatacg atctcgtcct cctcgttcag ggcgatgaga tcgatggggc
     2881 ggtcggagat ccggggaggc aggtagtccg ccaccacgcg gaaccccatg tccttgagtc
     2941 tcccgaacca gaagcactgc agggtccttc ggaccgtgtg atcattgcga aaatgcctgg
     3001 gcatctcttt ccacgccccc tccaacagtc ctttccattt ccgcaacaac tccgaaatct
     3061 tctcttccat ccccttcccc tttcccgtga tctgcagccg gattgccgag gcaactctac
     3121 ctaccgaaaa actcgctccg tgtcaaaccc ttgtgacttc aagcggatag aatgaacaag
     3181 ccatccggcc ttaacagaga gttgacagac atggggtctt gtgctacaag tcggtcaata
     3241 cgaaacatgt ccggagtttc cgaaagcacg gggggggtta agggagtcat tctcataaca
     3301 tcctagtttt cattaattaa gagaagaggg gcataaggag gcggcacgac cgagctcata
     3361 gcacacatcc aaggccggcg aggaacgatt tccttggaag aggcccgagc cggggtgaag
     3421 tgcgaaggat actcttgtcc ttgatccggc ggtgacgacg ccggcaatga agccgccaaa
     3481 ccggaatagc cccgaactgc tttcccggat ccacgggccc ccgcgaccgc accttctcga
     3541 cgggcagaat acgtcgcctg taatcgagtt ggcggagcta tataaaagca aaggaccatg
     3601 ccgccttcca tcctcattct cggcgacgtg atgctcgacc agtacgtgtg gggggaggtg
     3661 cgtcgcgtat cccccgaggc cccggtcccg gtggtccggg tttgtacccg aacccacgga
     3721 ctgggcgggg ccgggaacgt ggcggcaaac ctggcggggc tcaaagcgga cgccttcttg
     3781 ctgggggttt gcggggatga ttcggcggga gtccgcctga gggcgctcat cgaggccaac
     3841 ggcatccggg gtagcctgtc ggtcgacccg tccagaaaga ccaccaccaa gacccgggtc
     3901 atggcccagg gccagcaact catgcgcctg gacgaagaag attccatccc ggtttcccct
     3961 tccctggagg acgcgctcct cgatgcggtc cgggagcagt tgccgggttg cggggccgta
     4021 attctttcag actacggcaa aggcatcctc acatccctgg ggtttgtccg gcagctcatc
     4081 gaatccgccc gcacgtcagg ggtcccggtg ctggtggacc ccaagggaac cgattgggaa
     4141 cgataccgtg gagccgcctg catcactccc aatctttccg agctccaggc ggtggctcaa
     4201 ccttttcccg ccgaagacga cgctcaggtg gccggggcag ccgggaaagt cctggaaaaa
     4261 ttcggcctgg accggatcct ggtgacccgt ggagcccgag gcatgtgcct cctggaaaaa
     4321 gactcgaaac ctcatttcat ccggtccgcg gcccgacagg ttttcgacgt atcgggtgcc
     4381 ggagacacgg tcatcgccac cctcgcttac ggcatcgccc ggggagattc cttcgagcag
     4441 tcggcccggc gggccaacct ggcagccggg atcgtggtgg gaaaactggg tacccagccc
     4501 atattgctcc ccgagctcga ggcggccgtc caggccgagg agaccggctc cgtttccccg
     4561 tacccccgca agcttgcctc tcttgaggac gcccgggcga ggattctcca gtggcggtcc
     4621 accggggccg tgatcgtctt taccaacggc tgtttcgacc tactccaccc ggggcacatt
     4681 catctcctca agcaggcgcg atccctgggg gatcggctga tcatcggact gaactccgac
     4741 gaatccgtcc ggaggctcaa aggccccgcc cgtcccatcc tttcggaaca cgaccgggcc
     4801 gccgtcctgg gagccctgga agccgtggac ctcatcgtgg tcttcgggga agacactccc
     4861 ttggagctca tctcggccct gcggccggac atcctggtca agggctccga ctacaccctc
     4921 gacaccgtgg tcggccgcga cctggtggaa tcctacggcg gccgagtccg tctggtcccg
     4981 ctcctcgacg ggcatagtac gacgaggttg gtgaggatgg ttggttagtc gaggagttgg
     5041 ttggttgggg agttgggggg cgagggggca tccggcaggt gtgtgatata ggcccggggt
     5101 ctggagacaa ttctgaattg gtgattgtgg gttcaaccaa acggtttgaa gatttggaat
     5161 gccggcgtga agctcggaat ttcgtcggca tggtagacgg gcttgtgtcg aaggacaggt
     5221 ttcgaaagga ttatgatctg gcgagtcagg tcaaacgcag tgctgtttcc gccatggcaa
     5281 acatcgcaga gggatttcat cgcaggggca accgagaatt catcaagttt ctcgactgtt
     5341 cgcgatcttc cctggcggaa accctcagcc gccttcatgc ggcttcagac ctgcaatata
     5401 tcgaagaggc ggaaatgaat accctccgcc ggcagacgga tcttgtccgg aagaaggtga
     5461 acaatctcat gacctacctc caccaaacca accggccaac caattgaacc aaccaatgaa
     5521 ccaaccaact aaccaactaa tcaactaacc aaaccgggac ttcccatgaa tccaatccat
     5581 tcatctcaac cttccacctg ttccccttcc cccggacctt cgatttccgt ctgccctgcg
     5641 ggggaggctt ttcccatgcc ggggcccgag gactacgagg cggagctcaa gcggctccag
     5701 aagctcgttg cggaacagcg gcttctcggc cgcgagatcg tggtggtcat gggggtcggt
     5761 tttgtggggg ccgtcatggc cggggtggtg gccgattccg tcgaccgctc cacgggccgt
     5821 cccaacaagt tcgtcatcgg catgcagcgg ccgtccgttc gttcctattg gaagattccg
     5881 tacctcaacc ggggaatcgt acccgtccag gccgaagacc ccgaggtcgc gcccctgatc
     5941 cggcgctgcg tcctcgagaa gaaaaccctt acggcgactt tcacctatga tgtcctttcc
     6001 ctggcggacg tagtggtggt ggacgtccag tgcgactatc tcaaggaaac tcttgggaac
     6061 gtgcgccagg gcaaggccga catcggggca ctcgaggaaa gcctccgagt cctgggggaa
     6121 cgaatccagc ccgactgcct ggtcctcatc gaaaccaccg tgccgccggg caccaccgag
     6181 tacgtggcct acccgatcct caagagggcc ttcgaacgac ggggactgaa cgattccgag
     6241 ccgcttctgg cccactcctt cgaacgggtc atgccgggcc gaaattatgt tgcgtccatc
     6301 cgcgatttct ggcgggtgtg cagcggaatc aacgaaacgg cccgcaagcg ggttacggct
     6361 ttcctgtccg acgtcttgaa cgttgaccgg tatcccctca ccgtcctgga ccgccccatc
     6421 gaaagcgaga cgtgcaagat cgtcgaaaac tcgtaccgtg cgaccatcct ggcctttttg
     6481 cacgaatgga gcctttttgc cgagcgaaac ggggtggacc tcatcaaggt gatcgaggcc
     6541 atcaaggttc ggccgaccca ctcgaacatg atctttcccg ggcccggcat cgggggctat
     6601 tgccttccca aggacggggg actgggggtc tgggcctatc acaccctcat ggggttcgag
     6661 gacaacatct tcaagatcac gcccctggcc atcgacatca acgacacccg ggccctccat
     6721 gtcacccagc tggtccggga cgcccttcga aacatgggaa agatcgtggc cgcatccaag
     6781 atcgccctgc tgggggcttc ctaccgagag gatgtgggag acacccggta cagcggatcc
     6841 gagctcatcg tccgcaagtt ggtcgagatg ggcggggacg tttcggtcta cgacccttac
     6901 gtgcgccgct ggtgggaact ggagaaacag gacacctacc ctgccccggg gctttcctgg
     6961 gcgcggtttt tccggaacca ggaacggctc cgggagcttc gcatcgccga caccatggcc
     7021 cgggcgctgc aggatgcgga cgccctggtt ctcgccgtgc gccacaagga ttttctcgag
     7081 ctcgatcccg aggaggtggt ggaacacgca cggggtccct tggctgtggt cgattgcttc
     7141 ggcattctac acgacgagaa gatccggcga tttttcgaac tgggctgcga agtgaagggc
     7201 ctgggtcgcg gccacattca gcggatcaag gaaaccgtaa agaaaggcag gcgttaggtc
     7261 gtgcggttct tcaacctccc ccgcgaggag cgggaagagg tgcttatggc ggtatcggaa
     7321 atcttgcggg agcgatccga ccatggagtg gctgtgccgc gtggtggctc gatacctgga
     7381 catgaaacgc atcttggatc accaagcccg ggaggctttt g
//
LOCUS       DAOOVC010000183         7147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6270_length_7147_cov_6.633672, whole genome shotgun sequence.
ACCESSION   DAOOVC010000183 DAOOVC010000000
VERSION     DAOOVC010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6270_length_7147_cov_6.633672"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="acs"
                     /locus_tag="PLM79_16105"
     CDS             <1..1393
                     /gene="acs"
                     /locus_tag="PLM79_16105"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HOV87883.1"
                     /translation="DEGLRGGKPIPLKANTDEAVAKCPTVEKVIVVKHTGGRVPWTPG
                     RDLEWGKLMEEASADCPPEEMDAEDPLFILYTSGSTGKPKGVLHTTGGYLLYASMTHQ
                     YVFDYHDGDIYWCTADIGWVTGHSYIVYGPLANGATTVMYEGVPNYPDWSRFWNVVDK
                     HGINTLYTAPTAIRALMRQGEGPVKKTSRKSLRLLGSVGEPINPEAWLWYFNVVGDGR
                     CPIVDTWWQTETGGILITPLPGATALKPGSATLPYFGVKPAIIDPDGHVLEGPAQGYL
                     VITDSWPGMLRTVYGDHDRFKQTYFGAYKGVYFTGDGARRDSDGYYWITGRVDDVINV
                     SGHRLGTAEVESALVAHPTVAEAAVVGYPHDIKGQGIYAYVTLVAGLQPSEQLRKELI
                     QWVRKEIGPIASPDYIQWAPGLPKTRSGKIMRRILRKIAANEISDLGDTTTLAEAAVV
                     EDLIKNRQAALQG"
     gene            1821..2771
                     /locus_tag="PLM79_16110"
     CDS             1821..2771
                     /locus_tag="PLM79_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HOV87884.1"
                     /translation="MRRTGLLYDERFLLHRTGPGHPESHDRLALAHNALKEAGLLEKL
                     TLIEATPTRLKWIERVHSPRHIYRFEEACMLDLGELDHPDNQISPDSYDTALLAVGGI
                     LKTLDLVVKGQLDNAFCAVRPPGHHAESNRPLGFCYFNNVAVAAKYLQEQWGIQRVGI
                     VDFDAHHGNGTQAIFLEDPTVFYYSIHEHPSFSYPGTGRDFERGVGAGEGFTLNSLML
                     PGQGDEECKRLLQRDLFPAFASFKPEVILVSAGFDAHTDDDMSNLNLTSHGFAWIMER
                     LLELAREHSNGRLISILEGGYSLKALPDLIQDHVKILMSK"
     gene            2805..3497
                     /locus_tag="PLM79_16115"
     CDS             2805..3497
                     /locus_tag="PLM79_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HOV87885.1"
                     /translation="MFIDKSMTTDVITIRPETSIAEASELLVRNRVRHLPIIDEDGKL
                     LGIVTDRDIRSAMPSTMSVGEEADRERERISRLEVRDIMTRKVVTLSPMHTLEDALLL
                     MQRTKVGAFPVVDPDGRLVGILSVRDLMRAFINVLGIEEPGTLLGIVVEDKIGQMKRI
                     VDAITEEGISFGSILVARIWEEGKRAVFPYLLTHNVGRVKAKLEDLGFTLLNPMQWSL
                     HQLPKKTDKGSA"
     gene            complement(4016..4393)
                     /locus_tag="PLM79_16120"
     CDS             complement(4016..4393)
                     /locus_tag="PLM79_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87886.1"
                     /translation="MKKLAAGLVVVFVLSIIPAVQALAAPDLLGTYTGQANASAKSGY
                     VQTPATIKITSQKGGLFRGSMKLMNQSVPLYGFLASDREFRAVSEYVVVNGKFSSDYT
                     KISFAVQCVRPEEMYAAKGSVSR"
     gene            complement(4709..4984)
                     /locus_tag="PLM79_16125"
                     /pseudo
     CDS             complement(4709..4984)
                     /locus_tag="PLM79_16125"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            complement(4981..5415)
                     /locus_tag="PLM79_16130"
     CDS             complement(4981..5415)
                     /locus_tag="PLM79_16130"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOV87887.1"
                     /translation="MQRAEAEVFLMENVPQLLGSSEHEEILRGAEALGFACRWAKLCA
                     ADYGVPQARWRAFIVGSRMGDPAPFFPPPKTHFNPADGLPKQSEYVPNPAPWRTVRDA
                     VGDLPLPVKVRIREEPPLRTCISAEIPPSSAFVDTGRYPKKA"
     gene            complement(5740..6306)
                     /locus_tag="PLM79_16135"
     CDS             complement(5740..6306)
                     /locus_tag="PLM79_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87888.1"
                     /translation="MSNPGRAAVAALLLVILSSTGAWGGMPEFCRSDSNADAAACFGT
                     HPPYCSSSSYGDRGACFLANPGYCSSDSNADSAACFASHPSYCSSASYADRGACFAYG
                     GSYCSSDANANTLACFASHPSYCNSTSYADRLACSGARPSYCENENYASSKACAGPGK
                     PTQRQIWETARRRGTPVNVRKLAAELMR"
BASE COUNT         1464 a         2264 c         2134 g         1285 t
ORIGIN      
        1 ggacgagggg ttgcgcggcg gcaagcccat tcctttgaag gccaataccg acgaggccgt
       61 ggccaagtgt cccacggtgg agaaggtgat cgtggtgaag cacaccggcg gacgggttcc
      121 ctggaccccg gggcgcgacc tcgagtgggg caagctcatg gaggaagcct ccgcggattg
      181 cccccccgaa gaaatggatg ccgaggaccc tctcttcatc ctctatactt ccgggtctac
      241 gggaaagccc aagggagtgc ttcacaccac gggaggctac ctgctttatg cctccatgac
      301 ccaccagtat gtcttcgact atcacgacgg ggacatctac tggtgcaccg ccgacatcgg
      361 gtgggtgacg ggtcacagct acattgtgta cggtcccctg gccaacggcg caaccacggt
      421 gatgtacgag ggagtgccca actaccccga ttggtcccgg ttttggaacg tcgtggacaa
      481 acacgggatc aacaccctct ataccgctcc gacggccatt cgagcactca tgcgacaagg
      541 ggaagggccc gtcaagaaga cctcccgcaa gtccttgcgc ctcctgggaa gcgtgggaga
      601 acccatcaac ccggaagcat ggctctggta tttcaacgtg gtgggagacg gccgttgtcc
      661 catcgtggat acctggtggc agacggagac ggggggaatt ctcatcactc cccttcccgg
      721 cgccacggcc ctcaagcccg gttccgcaac cctgccctac ttcggcgtga aaccggccat
      781 catcgatccc gacggccatg tcctcgaggg ccccgcccag gggtacctgg tcatcaccga
      841 ttcgtggccg ggaatgctgc gcacggtcta cggcgaccac gaccggttca aacagaccta
      901 ttttggcgcc tacaaggggg tttacttcac cggggacggc gcccgccgag actcggacgg
      961 ttactactgg atcacgggac gcgtggacga cgtcatcaat gtgtcgggtc atcgattggg
     1021 cacggccgaa gtcgaaagcg ccctggtggc tcaccccacc gtggccgaag cggcggtagt
     1081 cggatatccc cacgatatca agggccaggg aatttacgcc tacgtgaccc tcgtggccgg
     1141 cctgcagcct tccgaacaac tgcgaaagga gctcatccag tgggtgagga aggaaatcgg
     1201 acccatcgcg tcgcccgact atatccaatg ggcgcccggc ttgcccaaga cccggtcggg
     1261 gaaaatcatg cggcgaatcc tacgcaagat cgccgccaat gaaatctccg acctggggga
     1321 caccaccacc ctggcagaag ccgccgtggt ggaagacctc atcaagaatc gtcaggcggc
     1381 cctccagggc tagagctcat gccatggccg ggggcggagg aaggcctctc ctcccccggc
     1441 atccccccaa ggttgtttga ttggcgtccc cccggaggcg gccgggggcc gaaaagcccc
     1501 gtgggttccg gatttcgccc gaacgacggc agcggtttca caccacccca ttccccccag
     1561 ggctcctggg cgggaaatcc ccccgtccaa cccccatctt tcgcggtttt tcccctccct
     1621 tccccccatg gcaaatgccg cggaaagctc cggggtcctt ttcccggccc cggaggcaac
     1681 ccatgatcca tagatgcttc ccgtcaatcc caacctgcaa gaggcggatc cccgggtttg
     1741 gataacgcat ttttagccgg gcgccaaata cggtatactg catggggact gtggatcggc
     1801 cgccaacgag gaaacctccc atgagaagaa caggtctctt gtacgacgag cgattcctgc
     1861 tgcaccggac gggccccggg caccccgaga gccacgatcg gcttgccctc gcccacaacg
     1921 ctctgaagga agcgggccta ttggagaaac tcaccctcat cgaagcaacc ccgacgcgcc
     1981 tgaaatggat cgagcgggtg cattcgcccc ggcacatcta tcgtttcgag gaggcctgca
     2041 tgctcgatct cggggagttg gaccacccgg acaaccagat ctccccggac agctacgaca
     2101 ccgccctcct ggccgtcggg ggaattctca agaccctcga cctggtcgtg aaggggcagc
     2161 tggacaacgc attttgtgcc gtgaggcctc cggggcatca tgcagaaagc aaccgtcccc
     2221 tgggcttctg ctacttcaac aacgtggccg tcgcggccaa gtacctgcag gagcagtggg
     2281 gaatccaacg ggtcggcatc gtggacttcg acgcccatca cggaaacgga acccaggcaa
     2341 tcttcctcga ggatcccacg gtattctact actcgatcca cgaacaccct tccttctcct
     2401 acccggggac cggccgggac ttcgagcggg gcgtgggagc gggggaaggc ttcaccctca
     2461 attccctgat gcttcccgga cagggggacg aagaatgcaa gcggctcctg caacgagacc
     2521 tcttcccggc atttgcttcc ttcaaacccg aggtgatcct ggtttcggca ggttttgacg
     2581 cccacacgga cgacgacatg tccaacctca acctgacttc ccatggcttt gcctggatca
     2641 tggaacgtct gctcgaactc gcccgggagc actccaacgg cagactgatt tccatcctgg
     2701 aaggcggcta tagcctgaaa gcgctgcccg acttgatcca agaccacgtg aaaatcctga
     2761 tgagcaaatg aaattcccac ctccttcgac caaaggacga caagatgttc attgacaaat
     2821 ccatgacgac ggatgtcatc accatccgcc ccgagaccag catcgccgaa gcctccgaac
     2881 tcctggttcg taaccgggtg cgccatctgc cgatcatcga tgaagacggc aaattgttgg
     2941 ggatcgttac ggaccgggat atccgcagcg ccatgccctc gaccatgtcc gtgggggagg
     3001 aagcggacag ggaaagggag cgcatttccc ggctcgaggt ccgggatatc atgacccgga
     3061 aggtggtgac cctttccccc atgcacaccc tcgaagacgc cctgctgctc atgcagagga
     3121 cgaaggtggg agccttcccc gtggtcgacc ccgacggcag gctggtgggc attctatccg
     3181 ttcgagacct catgcgcgcc ttcatcaacg tgctgggcat cgaggaaccg gggaccctgc
     3241 tgggaatcgt ggtggaggac aagatcgggc agatgaagag gatcgtggac gccatcaccg
     3301 aagagggcat atccttcgga tcgatcttgg tcgcccggat ttgggaggaa ggaaagcgag
     3361 ccgtcttccc ctacctgctc acccacaatg tgggccgcgt gaaggcaaaa ctggaggacc
     3421 tgggattcac cctgctgaat cccatgcaat ggagcctcca ccagttgccc aagaaaacgg
     3481 acaagggatc ggcgtgagca cccggggcgg gaacgctgtg ggaagggacc ccgagctgag
     3541 cgaatccgaa agcatcaggg cgttatgccc gcgacggcgg gtatggggcg ggtatgcacg
     3601 ctctttccgg attcccgcgc gcgcgggaat gacgtctccc ccacggaacc cactcctcgc
     3661 catccactcc ggattcccgc atgcacggga atgacaagac gggattcgta ccagacaaat
     3721 cgttcaatcc aggttaacct caccccggcg gtggccggaa tccggaaaga tgcatggaag
     3781 ccggattccc gctttcgccg ccaggacggc agcggctcaa tccaaccgca ttgaaccggg
     3841 gcgggggcct gcatcttttc gagacggcgg gaaaggatgc ccggggaccc ttggggaccc
     3901 gtagcccccc gttcccccgg gggaccgatc gaggagggag gccgacccca tttcccggtc
     3961 catggctgtc gacccgggaa tgagccggcc ctggagacct tcttgcccga agctgctacc
     4021 gggaaacgct ccccttggcg gcgtacattt cctccgggcg aacacattgg accgcaaaag
     4081 aaatcttcgt gtaatccgag ctgaacttcc cgttgacgac cacatattcc gataccgcgc
     4141 ggaattcccg gtcggaggcg aggaatccgt acaggggaac ggactggttc atcagtttca
     4201 tggacccacg aaacagaccg cccttctggg acgtgatctt gatggttgcc ggcgtctgaa
     4261 cgtagccgga cttggccgat gcgttggcct ggccggtata ggttcccagc agatccgggg
     4321 cggctagggc ctgaacggcc gggatgatgg acaggacaaa cacaaccacg agacctgcag
     4381 caagtttctt cattcctctt cccctttcct tgtgcgccat ccgaaccgat tccacctttt
     4441 tccatccgtc gaccgcggga aaactcccgt tcctcgcggg gggccgaatt cgacgaaacg
     4501 cccgacattc ccgccgatgt aagccgtttc cgcctccggg agaaggggcg gcgcctcgtc
     4561 gcatgcccgg gatttcattc ccgggccccg gcccaccata ccagcagagg accgcgaggt
     4621 gcaatagggg atcccccccg gcatcaagga ctgctcccgg gtggccgatc gagaaggcga
     4681 agcccgggat cgggccaagc ggaaccgcgt tcccgatctg ccgggcgatt tccatcttgg
     4741 atccgcgaaa ccggaagtcg tcgggaaacg cctggacccg ggcggcctcc cggtgggtga
     4801 tgggcccatg ttgagccgga taaaggcagc ggcccttccc gggcttgaag aactcggtgc
     4861 ggatggtaca cgcgggccga tcccaccaca accttccgaa aagatcggtc cccccggatt
     4921 cctttcgaat ccggcaggcg ggggtcaagt gaagggcccg gcgctgaagg tcgaaccgtt
     4981 tcatgccttc ttcggatacc gcccggtatc gacaaaggct gagctgggtg ggatttctgc
     5041 cgaaatgcag gtccggaggg gaggctcctc ccggatgcgg actttcacgg gaagaggaag
     5101 atcgccgacg gcgtccctta cggtcctcca tggcgcgggg tttgggacgt attcggattg
     5161 cttcggcagt ccgtcggcgg ggttgaagtg ggttttgggg ggtggaaaga acggcgccgg
     5221 gtctcccatg cgacttccca cgatgaaggc cctccatcgg gcctggggca cgccgtagtc
     5281 ggcggcgcac agtttggccc atcgacacgc aaaacccagc gcctccgcgc cacgaaggat
     5341 ctcctcgtgt tccgaagacc ccagaagctg gggaacgttt tccatgagaa aaacctcggc
     5401 ctccgctctt tgcacgacct ccgggaacgg gcgtcacagc ggcttgcggg aatcccgggt
     5461 gcgctgcttg ttgagaaggc tgaacccctg gcacgggggc ccccgatcac cacgtcggcc
     5521 gcgggaatct tcccaccggg atgatcgaga acggcaagaa tgtctccgtg aacgcagtgg
     5581 tttccgaaat tgcggttgta gtctcggcgg caggcccgtt gaaatcgttg gcccaggcgg
     5641 gcaggaaggg acggccgaat gccggggaaa accccagggt gagtcctccg gccccggaaa
     5701 aaaggtcgat caaccggtat tggacgtggt ttatggcccc tacctcatca actcggcggc
     5761 cagtttccgc acgttgacgg gagttccccg gcggcgcgcg gtttcccaga tctgtctttg
     5821 ggtgggcttg ccgggtcccg cgcaggcctt cgagcttgcg tagttttcgt tctcgcagta
     5881 cgacggcctg gccccggaac aggcgagccg gtccgcgtag cttgtgctgt tgcagtagga
     5941 aggatgggat gcaaagcagg ccaacgtgtt ggcattggcg tcgctggaac agtaggaccc
     6001 gccgtacgca aagcaggctc cgcggtcggc gtaactggcg ctgctgcagt aggacgggtg
     6061 cgacgcgaag caggccgccg aatccgcgtt gctgtcgctg gagcaataac cgggattggc
     6121 aagaaaacag gccccgcggt cgccgtagct cgagctgctg cagtacgggg gatgggtccc
     6181 aaagcaggct gccgcgtctg cgttgctgtc gctcctgcag aactccggca ttccgcccca
     6241 ggcacccgtg gaagacagga tcaccaataa aagtgccgct accgcagcac gccccgggtt
     6301 cgacatggtc cacaccctcc cttcccggcc cgaaggctcc cggaggcaca accccccggc
     6361 tcctcccggc cgaacaacca ccaaagccgc ccacggcccc aaatgcgcca acccgcgatg
     6421 aaaaacccct cacgccgccg acggtgccgt tcaatagagt caatccgctc ccgtatccgc
     6481 accttactca gctatacctc atagatttga gccgccaatg gaactatttt acccgaggca
     6541 cagccagctc ctctcaaccc tgatgattcc ccaaaaagtc gttttcccaa aacggcacat
     6601 aatgaattcg ggcggttata gactaacaaa cgtactcttc agggcttttt acgaaaccct
     6661 caatcttccg ttgagccttc ttctgtgtca tgttgtcctc catccagcgt aacgagagag
     6721 gatgtccgat tctcctggtg acatggtaag caccgtaact gaggagagtt gaagttcacc
     6781 cacccatggt ccgggtgtgg tccccaaagg gacctgcgcc cgccacccaa gtggggcgaa
     6841 gcccggagcg aagtgaaggg caaaacaaaa aatcgtgcgg tgacgtactc tgggagggta
     6901 ccacagaatt ccccccgagt gatcttgcgg ccgactccag ctcctggtaa ggaattcaac
     6961 tgcttatgcg agctccccct acgcgtttat acgtcaatac attattgccc gtcctccatt
     7021 cgctagaatc cgtgagttcg accagcatgc agtggggact acccgtgcgc aattgttcgt
     7081 gcaggctata tactttcacg actagcatca gcggccaaaa cagtagctct gtcgatgtga
     7141 acggtag
//
LOCUS       DAOOVC010000184         7103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6304_length_7103_cov_3.997020, whole genome shotgun sequence.
ACCESSION   DAOOVC010000184 DAOOVC010000000
VERSION     DAOOVC010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6304_length_7103_cov_3.997020"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2303
                     /locus_tag="PLM79_16140"
     CDS             <1..2303
                     /locus_tag="PLM79_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV87889.1"
                     /translation="LDSVGIQRIRSRIPTNHNPFYYYDRAIISIDTLKQDTEYRTYLE
                     NAYWDIIVIDEAHNVAERDGGSSLRAKLARLLAQRSDTLIMLSATPHDGKARSFASIM
                     NMLDPTAIANPDDYTREDIKGLFIRRFKKDIQNQVEKAFKERQIRQVPCRASVREEAA
                     FDAFVDLKFTRIDRTAPAGELFKTTLEKALFSSPAACLETIRNRVNRLRKSEDASWDE
                     DVQTLEDLAREVERITPEHFGKYRKLVELLRDTRHGLGWTGKDTRDRLVIFTERIETL
                     RFLQRHLKQELGLHENQVEVLYGTLSDIEQQRIVEDFGKEEAPVRLLIASDVASEGIN
                     LHFLCHRMIHFDIPWSLMVFQQRNGRIDRYGQEHTPQILYLVTHSDNAKIRGDTRILE
                     LLIQKDEQAVKNIGDPSALMGVYDIEEEEKITARAMEENQSPGEFEASLQAKAFDPLA
                     LLMGEEEPPTGSDAPAVTRSMPTLFRDDFAYLEAAVRHLRQSQPLQVEFLPERRRVDL
                     TPPEDLKQRFRFLPREIWPEDGVFTLSSDPATIQGEIKRSRKDENAWPNIHFLWTLNP
                     VLEWVNDKVVAAFGRHEAPVLGLQGALEPGEVVFILSGLIPNRRSHPLIHRWIGVSFR
                     NRHFHGIEDFQSLLDRTGLASRNFPNTGNDMDLEPLLKLLPEAVARAGAWMSRERKDF
                     EDRINEKLNEHLNALERLRKKQFLHLEARFESMQLPEKLVQGRKEKERREIDRIFDEY
                     FNWIEETMTTEDNPYIQVVAVLKGAD"
     gene            2303..7033
                     /locus_tag="PLM79_16145"
     CDS             2303..7033
                     /locus_tag="PLM79_16145"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOV87890.1"
                     /translation="MAMNLTGITNENEFYSHHYLSAILENDLKDVFKEWKRRDEEEGI
                     RPPYGELRTLARDFFAMRSRVGRERKAGERVAVQREFLQVLLQVLGYPFSLDLKELDD
                     GKRIPVMGEVKRPGGAPELWVLDALTTPGEDEDPLEVPLQAAQYPEGVEVDPGILEMS
                     LEDLITRRVFRLSEPPRWVILASDAQLLLLDRSKWNEKRLLRFDLKEIFDRKEPTTFQ
                     AMAALLHRESVCPSEGLSLLDTLDENSHKHAFAVSEDLKYALREAIELIGNEAVRYLR
                     EKLHEKVYERELAGDLTLECLRYMYRLLFLFYIEARPELGFAPMKADPYRLGYSMESL
                     RDLELVQLTTEESRNGTFLHESLELLFKMIYEGFPPEKDGPGRQLGMVAAPAHHTFRI
                     PPLRSHLFDPARTPILRKVKFRNQVLQKVIELMSLSRGGNRRDRRGRISYAQLGINQL
                     GAVYEALLSYRGFFAETDLYEVKKAGESHDPLNTAYFVKAEDLPKYSDQEKVYNEDGS
                     LRMYPRGTFIYRLAGRDREKSASYYTPEVLTRCLVKYALKELLKDRTADEILKLTVCE
                     PAMGSAAFLNEAVNQLAEAYLQRKQRETGRIIPHDRYGVEKQKVKMVLADNNVFGVDL
                     NPVAVELAEVSLWLNTIHEGAHVPWFGMQLVSGNSLIGARRRVFASELLKPAKRTDKV
                     WLDAVPTRIMPGEERPPKSVYHFLVGDRGMADYADRVVKEMAGDAVRAVNQWRRDFTR
                     PFTDREVSRLERLSGEIDRLWDRHTRDRRDVRRETTDSMSVFGMEGAEAKSGANRPAL
                     TTSEKDRRFLERIHSRGVRNSSAYRRLKLVMDYWCALWFWPMEKADLLPTREEYFFDL
                     SLILEGNVLETTPPEGQLRLFPDNRPKQLSMPAPDKFGMVDVERLCREIPRLEVVCEL
                     SARYRFLHWELEFAEVFADRGGFDLVLGNPPWIKVEWNEGGVMGDREPLFVLKSFTAP
                     QLAALRKETLARYDLRSDYLQAFEEAEGLQNFLNGLQNYPSLKGVQTNLYKCFLPQAW
                     MIGAKSGVSAFLHPEGIYDDPRGGPFREAVYARLRHHFQFENEMKLFQDVDNHGKFSV
                     NVYGECRPGAVGFSHLANLFVPPTVDACFEHHGGGAVPGIKDEANRWNVTGHASRIVP
                     VGEKELALFARLYDSEGTPALQARLPAVHSRELVSVLRKFADQPKRLGDLQGEYYSTV
                     MWDETNAVKAGTIRRETRFPESLGEWVLSGPHFFVGNPFYKTPRRQCGTNRDYDVLDL
                     TELPDDYLPRTNYVPACDPDEYLRRTPRVPWGDQEPVTHHFRLYARRQLSLSGERTLT
                     AAIVPPGAGHIHPVISTTFSDYYQLLQFCGLCNSLVYDFFIKTAGRGDLYESTLRYLP
                     LHEDIPTLVARALMLSCLTSHYANLWAGMFQDSFRQDRWAKSDPRLDNAKFANLTPQW
                     TRHCALRTDYERRQALVEIDVLTAMALGLTLEELKTIYRVQFPVLRQYEADTWYDRNG
                     RIVFTASKGLPGVGFSRPEWEPIKNMKSGTVPRTITDDTLPGGPRERTILYEAPFDRC
                     NREKDYETAWAEFERRFTAKQAVA"
BASE COUNT         1512 a         2314 c         2058 g         1219 t
ORIGIN      
        1 ggctggactc cgtggggatc cagcggatcc gcagccgcat ccccaccaac cacaacccct
       61 tctactatta cgacagggcc atcatctcca tcgacaccct gaaacaggac accgaatacc
      121 gaacctacct cgaaaacgcc tactgggaca tcatcgtcat cgacgaggcc cacaatgtcg
      181 cggaacggga cggcggttca tccctgcggg cgaagctggc caggctcctg gcccagcgct
      241 cggacacgct catcatgctc tccgccactc cccacgacgg aaaggcccgc agtttcgcaa
      301 gcatcatgaa catgctcgac cccacggcca tcgcaaaccc cgacgactac acccgggaag
      361 acatcaaggg cctcttcatc cgccgcttca aaaaggacat ccagaaccag gtggaaaagg
      421 ccttcaagga aagacagatc cgtcaggtgc cctgcagggc gtccgtccgg gaagaagcgg
      481 cgttcgacgc cttcgtggat ctcaagttca cccgcatcga ccggaccgcg cccgccgggg
      541 aactcttcaa gaccaccctc gagaaggccc tcttttcaag ccccgccgca tgcctcgaaa
      601 ccatccgaaa ccgggtgaac cgcctcagga agtccgaaga cgcctcctgg gacgaggacg
      661 ttcagaccct ggaagacctc gcccgggaag tcgaaaggat cactccggag catttcggca
      721 aataccgaaa gctcgtggaa ctcctccggg acacccggca tggtttggga tggacgggca
      781 aagacacccg ggaccgactg gtcatcttca cggaacggat cgagaccctg cgattcctcc
      841 agaggcacct gaaacaagag ctcgggctgc acgaaaatca ggtcgaggtg ctctacggga
      901 ccctctcgga catcgaacag cagcgcatcg tcgaggattt cggcaaggaa gaggcacccg
      961 tgcggctcct catcgcatcg gacgtggcct cggagggcat caacctccac ttcctctgcc
     1021 atcgcatgat ccatttcgac atcccctggt ccctcatggt ctttcaacag cgcaacggcc
     1081 gcatcgaccg ctacgggcag gagcacaccc cgcagatcct ctatctcgtg acccatagtg
     1141 acaatgccaa aatccggggg gacacccgca tcctcgaact cctcatccaa aaggacgaac
     1201 aggcggtgaa aaacatcggg gaccccagcg ctctgatggg cgtctacgac atcgaggagg
     1261 aagaaaaaat caccgcccgc gccatggaag aaaaccagag ccccggggaa ttcgaagcct
     1321 ccctccaggc aaaggcgttc gaccccctgg ccctgctcat gggagaagag gagccgccca
     1381 ccggcagcga tgcgccggcc gtgacccgct ccatgcccac actctttcgg gatgattttg
     1441 cttacctgga ggcggccgtc cggcacctca ggcagtcgca gcccctccag gtggaattcc
     1501 tccctgaacg gcgccgcgtt gacctcaccc ctcccgaaga cctgaaacag cgattccggt
     1561 ttcttccccg ggagatctgg cccgaggacg gcgttttcac cctttcgtcc gacccggcga
     1621 cgatccaggg ggaaatcaaa cggagccgca aggacgagaa cgcctggccg aacatccact
     1681 tcctctggac cctgaacccg gtcctcgaat gggtgaacga caaggtggtc gccgctttcg
     1741 gccggcatga agccccggtg ttgggcctcc agggtgccct agaacccggg gaagtcgtct
     1801 tcatcctctc gggcctcatc cccaaccgca ggagccaccc cctaatacac cgatggatcg
     1861 gggtctcgtt tcgaaaccgt cacttccacg ggatcgagga ttttcaaagc ctcctcgatc
     1921 gcaccggact ggcaagcagg aacttcccca acaccggaaa cgacatggac ctggagcccc
     1981 tcctcaagct ccttcccgaa gccgtggccc gggccggggc gtggatgagt cgggaacgaa
     2041 aggattttga ggaccgcatc aacgagaagc tcaatgagca cctcaatgcc ctggagaggc
     2101 tcaggaaaaa gcagttcctt cacttggagg cgcggttcga gtccatgcag cttccggaaa
     2161 aactcgttca ggggaggaag gagaaggaac gcagggagat cgaccgcatc ttcgacgagt
     2221 atttcaactg gatcgaagaa accatgacca ccgaggacaa cccctacatc caggtggttg
     2281 ccgtgctcaa gggggccgac tgatggcgat gaatctcacg gggatcacca acgaaaacga
     2341 gttctacagc caccactacc tcagcgccat cctggaaaac gacctcaagg acgtcttcaa
     2401 ggaatggaag cgccgggacg aggaggaggg catccgcccc ccatacgggg agctgagaac
     2461 cctcgcccgg gacttcttcg ccatgagaag ccgggtcggc cgggagcgga aggccggcga
     2521 gcgggtcgcc gtccagaggg aattcctcca ggtcctcctc caggtcctgg gctatccctt
     2581 ttccctcgac ctcaaggaac tggacgacgg caaacggatc ccggtcatgg gcgaagtgaa
     2641 acgtcccggt ggcgctcccg agctctgggt tctcgatgca ctgaccacgc ccggcgagga
     2701 cgaggacccc ctggaagtgc cgctccaggc ggcccaatat cccgagggcg tcgaggtgga
     2761 ccccggcatt ctcgaaatga gcctcgagga ccttatcacc cggagggttt tccggttgtc
     2821 cgagcccccc cggtgggtga tcctggcatc ggatgcgcag ctcctgctcc tcgaccgaag
     2881 caagtggaac gagaagcggc tccttcgctt cgacctgaag gagatcttcg accgaaagga
     2941 acccaccacc tttcaggcca tggcggccct tctccaccgg gaaagcgtgt gcccgtccga
     3001 agggctctcc ctgctcgaca ccctggacga aaactcccac aagcacgcat tcgccgtctc
     3061 cgaggacctc aagtacgccc tccgggaagc catcgagctc atcgggaacg aagcggtccg
     3121 gtacctccgg gaaaagctcc atgaaaaggt ctacgaacgg gaacttgccg gggacctcac
     3181 cctggaatgt ctgcgttaca tgtaccggct gctctttctc ttttacatcg aagcccgccc
     3241 ggagctcggc tttgccccca tgaaggccga tccctaccgg ctgggctaca gcatggagag
     3301 cctccgggac ctggagctgg tgcagctcac cacggaagag tcgaggaacg ggaccttcct
     3361 ccacgaatct ctggagcttc tcttcaagat gatctacgaa ggcttcccgc cggaaaagga
     3421 tggcccgggc agacagctcg gaatggtcgc cgccccggcg caccacacgt tccgcatccc
     3481 gcccctgcgc tcccacctct tcgacccggc gcgcactccc atcctcagga aggtgaaatt
     3541 ccgaaaccag gtgctccaga aggtcatcga actcatgtcc ctctccaggg gcggcaaccg
     3601 gagggaccgt cggggacgga tctcctatgc acagctcggc atcaaccagc tcggagccgt
     3661 ttacgaagcc ctcctttcct accggggctt cttcgctgaa acggacctct acgaggtgaa
     3721 gaaggccggg gagagtcacg atcccctgaa cacggcctac ttcgtcaaag ccgaggacct
     3781 ccccaagtac tcggatcaag aaaaggttta caacgaggac ggcagcctca ggatgtaccc
     3841 cagggggacg ttcatctacc gccttgcggg gcgggaccgg gagaagtcgg cttcctacta
     3901 cacccctgaa gtcctgaccc gttgcctcgt gaagtacgcc ctgaaggaac tcctcaagga
     3961 caggacggcc gacgagatcc tgaagcttac cgtgtgcgag ccggccatgg ggagcgcggc
     4021 gttcctcaac gaagccgtga accagttggc cgaggcctat ctgcagcgga aacagaggga
     4081 gacgggccgg attattcccc acgaccgtta cggagtcgaa aagcagaagg tcaagatggt
     4141 ccttgcggac aacaacgtct tcggggtgga cctgaatccc gtggcggtgg aacttgccga
     4201 ggtgtcgctc tggctcaaca ccatccacga gggggcccat gtcccctggt tcggcatgca
     4261 gctcgtgtcg gggaactccc tcatcggggc caggcgccgg gtctttgcgt cggagcttct
     4321 gaaacctgcc aagagaaccg acaaggtctg gctggatgca gttccgacga ggatcatgcc
     4381 cggggaggag cgacccccga aaagcgtata ccacttcctg gtgggggacc ggggcatggc
     4441 cgattacgcg gaccgggtgg tgaaggagat ggcgggggat gccgtccggg ccgtcaacca
     4501 gtggcgcagg gacttcacca ggccgttcac cgaccgggaa gtgagccggc tggagaggct
     4561 ctccggggaa atcgaccgcc tgtgggaccg gcacacgcgg gaccggaggg acgtccggcg
     4621 cgagaccacg gattccatgt cggtctttgg catggaaggg gctgaggcaa agagcggggc
     4681 aaaccggccg gcgctcacca cgtccgagaa ggaccgtcgc ttcttggaaa ggatccattc
     4741 caggggcgtc cgcaactcca gtgcctatcg gcgactgaag ctggtcatgg actactggtg
     4801 cgccctgtgg ttctggccca tggaaaaggc ggatcttctg cccacccgcg aggaatactt
     4861 cttcgacctg tccctcatcc tcgagggaaa cgtcctggag accaccccgc ccgaggggca
     4921 gctccggctt ttcccggaca accggccaaa acaactctcc atgcccgccc ccgacaaatt
     4981 cggcatggtg gacgtggaga ggctctgtcg ggagattccg cgcctcgaag tggtttgcga
     5041 gctgtcggcc cggtaccgct tcctgcactg ggaactggaa ttcgcggagg tcttcgccga
     5101 ccgcggaggc ttcgacctgg tgctggggaa ccccccgtgg atcaaggtgg aatggaacga
     5161 ggggggcgtc atgggcgaca gggagcccct ttttgtgctc aagtccttca cggccccgca
     5221 actcgccgcc cttcgcaagg agaccctggc ccggtatgac ctgcggagcg attacttgca
     5281 ggcattcgaa gaagccgagg ggctccagaa ttttctgaac ggccttcaga actacccgtc
     5341 cctcaagggt gtgcagacca acctctacaa gtgcttcctc ccccaggcct ggatgatcgg
     5401 ggccaagagc ggggtttcgg cctttctcca tcctgagggg atctacgacg atccgagagg
     5461 ggggccgttt cgggaagctg tttacgccag gctccggcac cactttcagt tcgaaaatga
     5521 gatgaagctc tttcaggacg tcgataatca cggaaagttc agtgtgaacg tctacgggga
     5581 gtgccgtcct ggggccgtcg gtttttcaca ccttgcaaac ctctttgtgc cgcccacggt
     5641 ggacgcctgt ttcgagcacc acgggggcgg cgccgttccg ggcatcaagg acgaggccaa
     5701 cagatggaac gtcaccggcc atgcttcgcg gatcgtgccc gttggcgaaa aggagctggc
     5761 tctctttgca cgtctctatg attcggaggg gactccggcc ttgcaggcgc gcctccctgc
     5821 cgttcattcc agggaactgg tgagcgtgct tcggaaattc gccgaccagc ccaaacggct
     5881 gggggacctt cagggggagt attattcaac ggtcatgtgg gacgagacca atgccgtcaa
     5941 agccggcacc atccggcgtg agacgcgatt ccccgagtcc ctcggcgagt gggtgctctc
     6001 agggcctcac ttcttcgtgg gcaacccctt ctacaagact ccccgccgtc aatgcggcac
     6061 caatagggac tacgacgtcc tggacctcac cgaactcccc gacgactacc tcccccgcac
     6121 caactacgtc cccgcctgcg acccggacga gtacctgcgg cgcacgcccc gggtgccgtg
     6181 gggggatcag gaaccggtga ctcaccattt caggctttac gcaaggcggc aactcagtct
     6241 ttcaggtgaa cgcacattga ctgcggcgat cgtgcctcct ggagcgggcc atatccatcc
     6301 cgtcatttcc accacctttt cggactacta ccagttgctg caattctgtg gtctgtgcaa
     6361 ctccctcgtc tatgacttct tcatcaaaac ggcagggaga ggagacctgt atgaatcgac
     6421 tctccgctac ttgccccttc acgaggacat accaaccttg gtagctcggg ctctgatgct
     6481 ctcatgcctg acaagccatt acgcaaacct ctgggcgggc atgtttcaag acagctttcg
     6541 ccaggatcgc tgggcaaaga gcgacccacg cctggacaac gccaaatttg ccaacctcac
     6601 acctcagtgg actcgccact gtgccctgcg caccgactac gagcgccgtc aggccctggt
     6661 ggaaatcgat gtcctcacgg ccatggccct ggggctcacc ctggaggaac tcaagaccat
     6721 ctaccgagtt cagttccctg tccttcgcca gtacgaggcc gacacctggt acgaccgcaa
     6781 cggccgcatc gtcttcacgg ccagcaaggg tctacccgga gtgggttttt cccggcccga
     6841 atgggaaccc atcaagaaca tgaaaagcgg gaccgtcccc cggaccatca cggacgacac
     6901 cctccccggc ggtccccggg aacgcaccat cctctacgaa gcccccttcg accgctgcaa
     6961 ccgggaaaag gactacgaaa ccgcctgggc ggaattcgaa cggcgattca ccgccaaaca
     7021 ggcggtagct tgacctcttg atgaacaatg tgacggaaat gggtgggcac aattgacttc
     7081 acggatatcg ccccaccccc cac
//
LOCUS       DAOOVC010000185         7052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6349_length_7052_cov_5.703873, whole genome shotgun sequence.
ACCESSION   DAOOVC010000185 DAOOVC010000000
VERSION     DAOOVC010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7052
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6349_length_7052_cov_5.703873"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..396
                     /locus_tag="PLM79_16150"
     CDS             76..396
                     /locus_tag="PLM79_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87891.1"
                     /translation="MKRSISVALALIMVVSVVGLSYAAGTIEEQIRGQERRIDQGVAK
                     GTLTPSEAETLRGNLDHIKDRYNKAVRDNILTKSEQNKLKKMLKNNSEMIYKKKHNQV
                     RRLY"
     gene            complement(494..1444)
                     /locus_tag="PLM79_16155"
     CDS             complement(494..1444)
                     /locus_tag="PLM79_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HOV87892.1"
                     /translation="MEDRIKLGVSSCLLGNKVRYDGGHKLDHFLADTLGAYVEYVPVC
                     PEVECGLPVPREAMRLVGDPSSPRLVTIHTGKDHTDRMLEWARRRVRELEQEKLCGFI
                     FKGRSPSSGMERVKVYGEGGMPAKTGVGLFARTFMEHFPLLPVEEDGRLHDPKLRENF
                     VEAIFTLKRWREFLDNRPNPGALVRFHSRHKLLLLSHSPRHYQSMGKLVARAGDISME
                     KLIPEYQSLLLEGLRLKTTGKKNLNVLQHLMGYFKNELTKDEKQELLEIFDLYARGTV
                     PLIVPITLINHYVRKYDQPYLREQHYLSPHPVELQLRNHV"
     gene            1915..2079
                     /locus_tag="PLM79_16160"
     CDS             1915..2079
                     /locus_tag="PLM79_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87893.1"
                     /translation="MKKPRIFIVEDEIVIVRGIEHALKRLGYETAGFALARGAGPRED
                     GGPEGRPGID"
     gene            2045..2287
                     /locus_tag="PLM79_16165"
     CDS             2045..2287
                     /locus_tag="PLM79_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87894.1"
                     /translation="MEGLKVALVLIDIFLAGKMDGIELSQEVAACHQVPFPYITAYSN
                     KEVLERAKGTHPSGYIVKPFRESRLKVAVELALAKE"
     gene            2638..2874
                     /locus_tag="PLM79_16170"
     CDS             2638..2874
                     /locus_tag="PLM79_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOV87895.1"
                     /translation="MSRLSAGLSPENEWSSALSASELRIAAMIKRGLNSEEIADHLSI
                     SPHTVRTHRRNIRKELQILNTHANLSIHLRGKQL"
     assembly_gap    3012..3111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3775..4650
                     /locus_tag="PLM79_16175"
     CDS             3775..4650
                     /locus_tag="PLM79_16175"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOV87896.1"
                     /translation="MSELTRDQLLSAVIAGRGPAYLRGVDVSSLDLSGVGWLVEADLR
                     SADLSHGNLSRAVLKNARLEGANLQGCNLVGANLEGADLRRARLNAANLRMANLAGAD
                     LQHARLVGSNLCAVNLTGANLEGADLEGANLEGADLREAKLQSANFHLANVVGVKLPS
                     LSPSARSQEVPERTSPDVGPHQGFLGSMQHIHLSDVIQLACLARSNLLFQIESPRGKG
                     TIHVRSGRVFHAQVDNLAGEKALFRMLQWDQGRFETFPLPDDAVVSIDKPIEHLLIES
                     MRHRDEIAAICAGDR"
     gene            4749..5138
                     /locus_tag="PLM79_16180"
     CDS             4749..5138
                     /locus_tag="PLM79_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOV87897.1"
                     /translation="MSLILTKGEVDQLDKIVRKELLDAGANHVMIVDMAGNLILERGS
                     LHMNDILALAVLSAANFAATAQIARLIGEEDFTLLFHKGDKRNIHFSRLDKEYILVTL
                     FDENVSLGLIRLRSGSAVEQLLNVFNK"
     gene            5151..5777
                     /locus_tag="PLM79_16185"
     CDS             5151..5777
                     /locus_tag="PLM79_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HOV87898.1"
                     /translation="MAFIDLSRGEIQVKIVYYGPGRCGKTTNLLFIHESMSKQVCGKM
                     VTIDTRGDRTLFFDFLPLGLGRIMNLNIKIQLYTVPGQVMYNSTRKLVLRGVDGVAFV
                     ADSLKVQKHKNIESLENLKENLADEGWDVRQIPLVLQFNKRDLAKSNIPILSVQEMEN
                     DLNSSLGVPCFEASALRGDGVYDTLREISKRTVKGVVRKMGGKLQQGM"
     gene            5854..>7052
                     /locus_tag="PLM79_16190"
     CDS             5854..>7052
                     /locus_tag="PLM79_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87899.1"
                     /translation="MLNPGNTEEKGNTAEIDLDAFDEQIDKEIDKLFTHPSENAGGAP
                     GRGTLGLNDAPPLSSGEVPPPRPFRPDSPSVELKSDPRAATAVPRPQPMAKPAEVFVL
                     ASEKPDQDEAVPVPGAVSGEQELGGFLEALSVAYLSLEWEISRENLVQLELALEKVEP
                     YCNRGPEAAALMKILKSLLQRLRARSSSTTPRVTEMVRDSYMLLRRILQNSSKATVQD
                     RKELNGLIARLRSLKEQSAAAVEKQGWESPPAPRVSADDSAVLSPSVLSSVPKEVPVP
                     AHGDLKTWLGGYWEEANAYMARAEEDAKRLAKIVEILDKVPALKAVSSHMARIRINLE
                     KQVLFQKEKNWEWVTQMERLLAPAERTPPVATQKSNGGEDLVSREASPAATQTPKAPG
                     ETEEGSRT"
BASE COUNT         1493 a         2027 c         2113 g         1319 t
ORIGIN      
        1 ggggggagtg gcggaacatc ggtacagcgt cgttttgacc gtctcaacaa catccggaaa
       61 aggaggagag gactcatgaa aagatccatc agcgttgcgt tggccttgat catggtcgtt
      121 tcggtggttg gactgagcta cgcggccggt accatcgagg agcagatcag gggacaagag
      181 agacgaatcg accagggggt ggctaagggg accttgactc cgtccgaggc ggagacgctc
      241 cgaggcaatc tggaccacat caaagaccgt tacaacaaag ccgtccggga caacatactg
      301 accaagtcgg aacagaacaa gctcaagaag atgctcaaga acaacagcga aatgatttac
      361 aagaagaaac acaaccaggt caggcgtctt tattaacctc taacctcctt tcatccatcc
      421 tgtccggagg cggagaccgg tccctgattg ctgggaggtc ctccgcctcc ggccttttct
      481 tcccctcgag gaatcacacg tgatttcgaa gctgcagctc cacgggatgg gggctcaggt
      541 agtgctgttc cctgagatag ggctgatcgt attttcgcac gtagtgattg atgagggtga
      601 tgggaacgat gaggggcacg gtcccgcggg cgtacaggtc gaagatttcc aggagttcct
      661 gtttttcatc cttggtcagc tcgttcttga aatagcccat gaggtgctga agcacgttga
      721 ggtttttctt ccccgtggtt ttcaggcgca atccctccag cagcagcgac tggtattccg
      781 ggatgagctt ctccatggag atatccccgg cccgggccac cagtttgccc atggactggt
      841 aatgcctggg gctgtgggag agcagcagca gcttgtgccg actgtggaag cggaccaggg
      901 cccccggatt gggtctgttg tccaaaaact cgcgccaccg cttgagggtg aagatcgcct
      961 ccacgaaatt ctcccgcagt ttcgggtcat ggagtcggcc gtcctcctcc accggcagga
     1021 ggggaaaatg ctccatgaaa gtccgggcaa agaggcccac tcccgttttg gccggcatgc
     1081 ctccttcgcc gtagactttc acccgttcca tgccgctgct gggagatctc cccttgaaaa
     1141 tgaacccgca gagcttctcc tgctcgagct ccctcacccg tcggcgagcc cattcgagca
     1201 tccggtccgt gtggtctttg cccgtgtgaa tggtcaccaa tcgcgggctc gaagggtctc
     1261 ccaccaacct catggcttcc cgggggacgg gcaggccgca ttcgacctcg ggacagacgg
     1321 gcacgtactc gacgtaggcg cccagggtgt ccgccaaaaa gtggtccagc ttgtgcccgc
     1381 cgtcgtagcg gaccttgttt cccagcagac atgagctgac tcccagcttg atacgatcct
     1441 ccatctgcca cctcttgatt ggatgaatgg tttcttgccc aggggcggga agccccttcc
     1501 gacgactcga ggggaccggc aggcacccta ccggaatcga ccgatcccgg caccgattgt
     1561 catcctatca tgaaaccccg acgtccgaaa ctcgagttcc ccccgtacgt gtcctcacag
     1621 gaaagacgga ccgggacgca tggagctgcc atcctgcgtt cgcccttcta tttttctccg
     1681 gaacattgcg attggcgggg ttttgtaaaa cagtgtcttc caatgaagag aatccctgca
     1741 atgggttgac cgaggctttg ggtctcttcg attgcgacac cgagcccagc cgccgttctt
     1801 cgaagatccc gcggggatga gtttcaagaa agtgtttggg atgtcgaagg gaaaagtccc
     1861 tcaaagtcat tgggtccaac gaccggccgc ggcgaatccg gaggtcgagt ccccttgaag
     1921 aaaccacgca ttttcatcgt tgaagacgaa atcgtcattg tgcgcggcat cgagcatgcg
     1981 ctgaagaggc tcggctacga gaccgcggga tttgccctgg cccggggagc aggccctcga
     2041 gaagatggag ggcctgaagg tcgccctggt attgattgac atcttccttg ccgggaaaat
     2101 ggacgggatc gaactttccc aagaagttgc ggcctgccac caggtcccat ttccatatat
     2161 cacggcctat tccaacaagg aggtcctgga gagggccaag gggacccatc cctcgggcta
     2221 tatcgtgaag cctttccggg agagccggct caaggtcgcc gtcgagttgg ccctggccaa
     2281 ggaataggag gatcgtagaa cgagggcggc catcgagggt taccggaaga cgatcctgga
     2341 tcttcaaagc gatctcgaac gtcaggcggc ggaagcggcg gcagacgccc gggagctcga
     2401 ggcggcgcac cagaaggtcg gggagcacga ggggaacgtc gatgagcttc ggtgtcggct
     2461 tttcgacctc aaccaggccc tgccggcctt gagtcggcag gtggagagga accgcaagga
     2521 catcgaactc gagattgccg aagccgtgcg gatgaaaata cagcccatgt tgcggaagct
     2581 tcaatcggac ccccccttca gagagaccgg atcgagaagg aaatgctgtc cccgtacatg
     2641 agccggcttt ccgccggctt gtcgcccgaa aacgagtgga gcagcgccct ttcggcgagc
     2701 gagcttcgca tcgcggccat gatcaagagg ggtctcaaca gtgaggagat tgccgaccat
     2761 ctgtccatct ccccgcatac ggtcaggacc caccggagaa acatccgaaa agagctccag
     2821 atcctaaaca cccacgccaa cctctcgatc cacctgcggg gaaagcagct ctgaggtcgg
     2881 ttcggaccgt tatgccgggc cgttccggtg gtgcctttat cgagaaatcc cgcttcccgg
     2941 agacgccggt tcgctgccgc gacatgcaga ccttgtggcc ctcgagatcc cgttgccgga
     3001 actcgcccgc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nccgcgacat
     3121 gcagaccttg tggccctcga gatcccgttg ccggaactcg cccgccgcct tcccggtccg
     3181 gcagagcgcg tagggaaata cctacttcat ccccccagaa accatgcttg accctcccat
     3241 cgtccccggg gcaacatcca tccaagttct caggcaagtc gtaaccgctt ccaaaagccg
     3301 agggggtggc catgaaaccc acgagccatg aaaagggaaa tcatggacgg gggtcccggt
     3361 ccgaggtttt cggtcgggcc gggcaaggtt ggcttgttgt cctggtgttc ggcgggaaca
     3421 tatccggaca agcaagcaga acttggcgag atccctttcc cgggtttggg tagcggggtc
     3481 gttgcccggg aaggaactcg gtcggtcggg acgaggcgcc aagggaccct tgttgcctga
     3541 aggatcgcca aaggatcatc ccccggagac cgctcctccc ggatagcatt tccggccccg
     3601 gtgtcaatga atcgtggaaa agggccgtga ccggcctgcc cggaggaccc tgaaaaaaaa
     3661 ctccttattt atttctgccc gttggacgac cttaggggat gaggggcatt gcacttggaa
     3721 ggattggtgt tccctcggga cgaaaatcga tgcgcattgc ggggtgggtg tgttatgtcc
     3781 gagttgacca gggaccagtt gctcagtgcg gtgattgcgg gaagaggccc cgcgtacctt
     3841 cgcggggtgg acgtctcatc cctggacctg tccggcgtcg gctggctcgt cgaggcggac
     3901 ctccgttctg cggacctttc ccacggcaac ctgagccggg ccgtcctcaa aaacgcaagg
     3961 ctcgagggcg ccaacctcca gggctgcaac ctggtgggcg ccaacctcga aggggccgac
     4021 ctgcggcgtg cgaggctcaa cgcggcaaac cttagaatgg ccaacctggc cggggccgat
     4081 ctccagcatg cccggctggt gggaagcaat ctctgtgcgg tgaatctcac gggagcaaac
     4141 ctcgaggggg cggatctcga gggagcgaac ctcgagggtg cggacctccg ggaagcaaaa
     4201 ctccagtcgg ccaacttcca cctggccaac gtggtggggg taaagctccc gtccctcagc
     4261 ccctccgcac gttctcaaga agtcccggaa cggacctcgc cggacgtggg tccccaccaa
     4321 ggtttcctgg gctccatgca acacattcat ctatcggacg tgatccagct ggcttgcctg
     4381 gcccggtcga accttctttt ccaaatcgag tctccccggg gcaaaggaac catccatgtc
     4441 cgctcaggca gggtctttca tgcccaggtc gacaaccttg cgggagaaaa ggccctcttt
     4501 cggatgctcc aatgggacca gggccggttc gaaaccttcc ctctcccgga tgatgccgtc
     4561 gtttccattg acaaacccat cgagcatttg ctcatcgaat ccatgaggca tcgagatgag
     4621 atcgccgcca tttgcgcggg ggatagataa tcccggggga ttgatgtgag aagggaaata
     4681 gggtccaatt cgagtgccca ggactccccg gtacgatccg aggatctaca ggaaaggttg
     4741 gagatagtat gagtctgatc ttaaccaaag gcgaagtcga ccagcttgat aagatcgtac
     4801 gaaaggaact cctggatgcc ggggcaaatc acgtgatgat tgtggacatg gccgggaacc
     4861 tgattctcga gcgaggctcc ctgcatatga acgacatcct ggccctggcc gtgctctctg
     4921 cggcgaactt cgccgccacc gcccaaatcg cccgactcat cggcgaggag gatttcacgc
     4981 ttctcttcca caagggggac aagagaaaca ttcacttcag caggctggac aaggagtata
     5041 tcctcgtcac cctcttcgac gagaacgttt ccctgggcct gattcgtttg agatccggca
     5101 gtgcggtgga acaattactg aacgttttca acaagtagaa ggaaatatcg gtggctttta
     5161 ttgatttgag caggggtgag atccaggtaa agatcgtgta ctacgggccg ggaaggtgcg
     5221 gcaagacgac gaatctgctc ttcatccacg agtctatgtc caagcaggtt tgcggcaaaa
     5281 tggtcaccat cgacacccgt ggagaccgga ccctcttctt tgactttctg cccctgggac
     5341 tgggaagaat catgaacctc aacattaaga tccagctcta caccgtgccg ggacaggtga
     5401 tgtacaactc cacccgaaag ctggttctcc ggggcgtgga cggggtggcc ttcgttgcgg
     5461 attccctcaa ggtgcagaaa cacaagaaca tcgaaagcct ggaaaacctg aaggaaaacc
     5521 ttgcggacga aggctgggac gtccggcaga tccccctggt gcttcaattc aataagagag
     5581 acctggcaaa atccaacata cccatcctga gcgtccagga gatggaaaac gatctgaatt
     5641 cgagcctggg cgtgccctgc ttcgaggcca gtgccttgag gggggacggg gtttacgaca
     5701 ccttgaggga aatcagcaag agaacggtca agggggtcgt gcgcaaaatg ggggggaaac
     5761 tgcagcaagg gatgtagagc ggggaggtcg ggcttccccg ggccccggcc gggtcccggg
     5821 gtgggtcccc cgaggagaaa agagggaaat tccatgctga atccaggaaa taccgaagag
     5881 aagggcaata cggccgagat cgatcttgat gctttcgacg agcagatcga caaggaaatc
     5941 gacaaactgt tcacccatcc cagcgagaac gccggggggg cccccggccg cgggaccctg
     6001 gggttgaacg acgccccgcc gctgtcgagc ggggaggtcc cgcctccacg cccgtttcga
     6061 ccggactcgc cttccgtgga gctcaagtcc gatccccggg cggcaaccgc agttccccgg
     6121 ccccagccaa tggcgaagcc cgctgaggtc ttcgtcttgg catcggagaa accggaccag
     6181 gacgaggccg tcccggttcc cggtgccgtg agtggggaac aggagctggg agggtttctc
     6241 gaggccctca gtgtggccta tttgagcctc gagtgggaga tctcccggga gaacctggtc
     6301 cagttggaac tggccctgga aaaggtggaa ccctactgca atcggggtcc ggaagcggca
     6361 gccctcatga agatcctcaa gtccctgctc cagaggttgc gggctcgatc ctcctccacc
     6421 acgccccggg ttacggagat ggtccgggat tcgtatatgc tcttgcgccg tatcctccaa
     6481 aattcctcga aggcgacggt gcaggatcgg aaggaactga acggtctcat cgcccggctt
     6541 cgctcgctca aggagcaatc ggccgccgcc gtcgagaagc agggctggga atccccgccc
     6601 gctccccggg tttccgccga tgattcggcg gtcctttccc cgtccgttct ttcttccgtc
     6661 ccgaaggagg ttcccgttcc ggctcacggc gacctcaaga cctggctcgg cggctattgg
     6721 gaagaggcaa acgcctacat ggcccgggcc gaggaagacg ccaagcggct tgccaaaatc
     6781 gtggaaatcc tggataaagt gcctgcgtta aaggcggttt cttcccatat ggcaaggatt
     6841 cgcatcaacc tcgaaaaaca ggtcctgttc caaaaagaga agaactggga atgggtcacc
     6901 cagatggaac ggctccttgc cccggcggaa aggaccccgc ccgtcgcgac gcagaagagc
     6961 aacggcggcg aagacctcgt ctcccgggaa gcgtcgcctg cggcaactca gaccccaaag
     7021 gcgccggggg aaacggagga agggtcccgg ac
//
LOCUS       DAOOVC010000186         6885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6508_length_6885_cov_5.972914, whole genome shotgun sequence.
ACCESSION   DAOOVC010000186 DAOOVC010000000
VERSION     DAOOVC010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_6885_cov_5.972914"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..608
                     /locus_tag="PLM79_16195"
     CDS             333..608
                     /locus_tag="PLM79_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87900.1"
                     /translation="MSNYTRVYDYKEIELAPGEQKGFKLACPEGTNALGGGAVAWPSQ
                     WAINATYPYDHRTWAVWFYSIPQEGAPATTTGRVGIYAVCAVVESMQ"
     gene            708..1313
                     /locus_tag="PLM79_16200"
     CDS             708..1313
                     /locus_tag="PLM79_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87901.1"
                     /translation="MKPSVHNEPSFLVVTDHPSLFHPLLQRGFLVGIREGCTLRDLLE
                     KRLGLSPDSVEEELGTIFLDGRPVDDLDATRLKDGSRLALSAAMPGLVGAALRRGGFY
                     AAFRSDISHREEDPVPGIREAVVWVKLFNLVARTQGPRVLEKGILLERNHLREFLEDH
                     TGDFRAHCRSVRVNGRETDPDGMLAAAGSPGSDRVRLRVLS"
     gene            1733..3070
                     /locus_tag="PLM79_16205"
     CDS             1733..3070
                     /locus_tag="PLM79_16205"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HOV87902.1"
                     /translation="MKRREKNLGAVALAVFLSVTVCMPVSGRGTCSEASETDLPVAGG
                     YTLTVKNNCDFDLYLRGADLSSRLVPDNQLLSAHGGSHQYQVPIPWTSGRIYGCWDDT
                     SKMDIIGGKNAYTMKQHCGFVEMTLKAGGVLSSNITFVDAISLPMRIEAPGGSACTNG
                     GSVTAAFNPADIVSKCPTKVLNNRACISAYYYCADFCRPGDRTCAAYCSKFDPVIKDC
                     KAHYPGCNAGNAVTKDVYGCTPTSFFSSAAGEKYCAAINRGILESYDKQTNPKTFYPQ
                     GKPYNDYSALVHKTVGPIFGFPYDDYPSRLNQGGYLNCQASSQYILTFCPNRGKLIYS
                     YLGDALSWTAWDGDVFRFSGTAEEEVVLRLEAVGDDARGRADLVVRGRDFQEKDRGEL
                     PLAVRLTLPHTGTYQVAVHNAAGGAKAYVGHYRLSIECSRNAWETLKPAHGVE"
     gene            complement(3346..3422)
                     /locus_tag="PLM79_16210"
     tRNA            complement(3346..3422)
                     /locus_tag="PLM79_16210"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3386..3388),aa:Asp,seq:gtc)
     gene            complement(3431..3506)
                     /locus_tag="PLM79_16215"
     tRNA            complement(3431..3506)
                     /locus_tag="PLM79_16215"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3471..3473),aa:Val,seq:tac)
     gene            complement(3594..6044)
                     /gene="lon"
                     /locus_tag="PLM79_16220"
     CDS             complement(3594..6044)
                     /gene="lon"
                     /locus_tag="PLM79_16220"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOV87903.1"
                     /translation="MFKLNRNKEDQQNPVSPVSLPLLPLRDIVVFPSIVVPLFVGRDK
                     SVNALDKAMSSEKRIFLAAQTKAKTDTPGESDIYRIGTIANILQILRLPDGTVKVLVE
                     GEQRARVLHFVPHPDYFHVQVEVLSEPPPNPIQVEALRRGIRSTFEAYAKHNKKITQE
                     ILDAVAAIDEPSRLTDTIASYVPFKLDIKQQILETVDVAQRMEKVFGHLRAEIEIGLT
                     EERIKGRVKKQMEKTQREYYLTEQMRAIQKEMGEKDDFKSELEELEKRIKRKKLSQEA
                     ATKVRAELKKLKLMSPMSAEATVVRNYIDWILSLPWYEKTRDKLDIEGASVILEEDHY
                     GLEKPKERILEYLAVQALVKKIKGPILCFVGPPGVGKTSLARSIARSMNRNFIRLSLG
                     GVRDEAEIRGHRRTYIGALPGKIIQSLRKVKSNNPVFCLDEIDKMSMDFRGDPSAALL
                     EVLDPEQNFAFNDHYLDLDYDLSEVFFITTANNLHSIPPPLRDRMEIIQIAGYTEFDK
                     LNIARNFLVSKQCKANGLQPGNVIFTDEILLYMIRHYTKEAGVRNLEREIASVCRKVA
                     KEVVRQGPETRIELTEELVQEYLGIPKYRYGRTEEKDEVGLAIGLAWTEFGGDILGIE
                     SIIMPGKGKMIITGKLGEVMQESAQAALSYVRSRSARLGIEPDFYQKFDIHVHVPEGA
                     IPKDGPSAGITMATSLVSALTRIPVRSDLAMTGEITLRGRVLPIGGLKEKILAAHRGL
                     LKKVLIPKDNAKDLKEIPAKILQEIEIEVIEHMDDVLVRALALKEGQELFREPEEVEG
                     KAVLFEAKEPPVDEIRAH"
     gene            complement(6157..>6885)
                     /gene="clpX"
                     /locus_tag="PLM79_16225"
     CDS             complement(6157..>6885)
                     /gene="clpX"
                     /locus_tag="PLM79_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOV87904.1"
                     /translation="RGIVYIDEIDKIAKKSDSPSITRDVSGEGVQQALLKILEGTVAN
                     VPPKGGRKHPQQEFIKIDTTNILFICGGAFNYLETIIQSRVGVKGMGFGAEIRSKEDR
                     NIGEILIQVQPEDLIKFGMIPEFVGRMPVIATLNELTEDELVRILTEPKNALVKQYKK
                     LFELEEVRLRFTEGALRAIAKEALRRKSGARGLRSIMENIMLDIMYDLPSHPEIQECI
                     IGEDVILKHETPLLLYRGQNQESA"
BASE COUNT         1232 a         2097 c         2088 g         1468 t
ORIGIN      
        1 aagagcaaca cacccccggg gacccgaaag ctgcacgtcc gactgggaga cgacccgaac
       61 gccaagctcc tgcccgtgga ggtgacggac ctggtgctgc cttcgggaga cgaggttccc
      121 gtcaccgtca tggccaatat ttccgtcatg ggttcggggg aggagcgtgt cgccatggcc
      181 cgggagcttc tcgagctcct ttcccggtac cggatcaacg gggctaaggt tgtatcggag
      241 aggaaccggc agcctccgac aagtggctcc ttcttgcccg gaaaggcgaa aatggagcgg
      301 atgggaagga tggtgaagac ggcaaggccg gaatgtccaa ctacacccgc gtttacgact
      361 acaaggaaat cgagttggcg ccgggggagc agaagggctt caagctggcc tgtcccgagg
      421 gaaccaacgc tctcgggggc ggggccgtgg catggccttc ccagtgggca atcaacgcca
      481 cctatcccta tgaccatcgg acctgggccg tgtggtttta ctccatcccc caggagggag
      541 ccccggccac caccaccggc agagtcggca tctacgccgt ctgcgccgtc gtggaatcaa
      601 tgcagtaaag gtttgcaccg atcgtcggag agagagcacc tcgcagcttc ccgcggagct
      661 ctcttgcccg cccgacaggc ttgccctcgg ccctcggaac gagaaccatg aaaccctccg
      721 tccacaacga accctctttc ctcgtcgtta ccgatcatcc gtccctcttt cacccgctcc
      781 tccagcgcgg cttcctggtc ggaatccggg aggggtgcac cctccgggac ttgctcgaga
      841 aacggctggg cttgagtccc gactccgtcg aagaggaact ggggacgatc ttcctggacg
      901 ggaggcccgt ggacgacctg gacgcgaccc ggttgaaaga cggctcccgc ctggcccttt
      961 ccgccgccat gccggggctg gtgggagcgg ccctgcgccg ggggggcttc tacgccgcgt
     1021 ttcgaagcga catctcccac cgagaagagg atcccgttcc ggggattcga gaggccgtcg
     1081 tttgggtaaa gctcttcaac ctggtggctc gaacccaggg accccgggtc ctggaaaagg
     1141 gaattcttct tgaaagaaac cacctgcggg aatttctcga ggatcacacc ggggattttc
     1201 gtgcccactg caggtccgtc cgggtgaacg gccgcgaaac ggacccggac gggatgcttg
     1261 cagccgcggg ttcccccgga tcggaccggg tccggttgag ggtcctctcc tgacacccgc
     1321 ctgcccggga cctcccgcct tcttccttct tcgataaccc atggacatgc aggaattttc
     1381 ccttcccggt ccttttccac tcgaggcatg agcgccaacc cctggcaggt ttttcccttc
     1441 ccggtctttc cccgttttcc ggggagaatc cggtggaatt ctttgccgca tccctccctc
     1501 gacggcgtgc ctcgactttt cccctccctt gacgggagcg gttcggggag ggggatgaat
     1561 gggcccgcaa tcttccaaga ccgacctccc ttttccttgc gcaaaaccgg ggatcatggt
     1621 ggaatccagc ctggggtcga gaaccggccc cgtcgcgtta cgggtgcttt tgttcccggg
     1681 cggctttcga atcgaagggc gttggcggca tggatcacgg aaggagggtg ggatgaaacg
     1741 cagggaaaag aaccttggag cggttgcttt ggccgtgttc ctgtcggtga cggtctgtat
     1801 gccggtttcc ggccggggta cctgcagcga ggcgtcggaa accgatctcc cggtggcagg
     1861 gggctatacc cttacggtca agaacaattg cgattttgac ctctacctgc gcggtgcgga
     1921 cctctcgagc cgcctggtgc cggacaacca gctcctttcc gctcacgggg ggtctcacca
     1981 ataccaggtc cccatcccgt ggacctcggg aaggatctac ggctgctggg acgacacctc
     2041 caagatggac atcatcgggg gaaagaacgc ctataccatg aagcagcact gcggtttcgt
     2101 ggagatgacc ctgaaagcgg gaggggtcct ttcctcgaac atcaccttcg tggacgccat
     2161 ttccctgccc atgcgcatcg aggccccggg aggaagcgcc tgcaccaacg ggggttcggt
     2221 gacggccgcc ttcaaccccg cggatatcgt cagcaagtgc ccgaccaagg ttttgaacaa
     2281 ccgtgcctgc atcagcgcct actattactg tgcggatttc tgccgccccg gcgaccgaac
     2341 ctgtgccgcc tattgctcga agttcgatcc cgtcatcaag gactgcaagg cccattaccc
     2401 cggctgcaac gcgggcaacg ccgtcaccaa ggacgtgtac gggtgcactc ccacctcctt
     2461 tttcagcagc gccgccgggg aaaagtactg cgcggccatc aaccggggca tcctcgaatc
     2521 ctacgacaaa cagacgaatc ccaagacctt ctacccccag ggaaagccct acaacgacta
     2581 ttccgccctg gtgcacaaga cggtgggtcc cattttcggg ttcccctacg acgactatcc
     2641 cagccggctc aaccaggggg ggtatctcaa ctgccaggcg tcttcccaat acatccttac
     2701 cttttgcccc aaccgcggca agctgatcta ctcgtacctg ggagacgccc tgtcctggac
     2761 ggcttgggac ggggacgtct tccggttttc cggcacggcg gaagaagagg tggttctcag
     2821 gctcgaggcc gtgggggatg atgctcgggg gagagcggac ctggtggtcc ggggccgcga
     2881 cttccaggag aaggaccggg gggagttgcc cctggcggtc cgcctgactc tgccccacac
     2941 gggcacctac caagtcgccg tgcacaacgc ggccgggggg gcgaaggcgt atgtaggcca
     3001 ctatcgcctg agcatcgagt gttcccggaa cgcctgggaa accctgaaac cggcccacgg
     3061 ggtcgagtag aaagaaatcg gggaaagaag ttgcccggcg ggggcggccc cggttcccgg
     3121 cccgtccggt cgagcctgga agcgtccatg cgtccttctt ttccggagag ccgaatccaa
     3181 tgacgggcaa gagtcgccaa gatccggctc accctcaccc tgagggagag ggaacccgtg
     3241 actcaaccgc atcagggatt tccgttcggg gatgaatcct cgatcctgcg gcaaccccgc
     3301 aaaacaaaaa ggagccgacc ccatgggccg actcccggat ttttctggcg gggtcgacga
     3361 gacttgaact cgcggcctcc ggcgtgacag gccggcgtta taaccaactt aactacgacc
     3421 ccgcatgtcg tggtgggcgg aacagggctc gaacctgtga cccccggctt gtaaggccga
     3481 tgctctccca gctgagctat ccgcccggga ccacctgccc ggccagcgtt tccgccttgc
     3541 aggttgaacg agggggaaat gtacttcgag gcccctgcaa agtcaagggt tttctagtga
     3601 gcccggatct cgtccacggg cggctccttg gcctcgaaga gcaccgcttt gccttccacc
     3661 tcctcgggtt cccggaaaag ctcctgtccc tccttgaggg ccagtgccct caccaggaca
     3721 tcgtccatgt gttcgatgac ctcaatctcg atctcctgga ggatcttggc cggaatctct
     3781 ttgaggtcct tggcgttgtc cttggggatg aggaccttct tgagaagtcc tcggtgtgcg
     3841 gcgaggattt tctccttgag tcccccgatg ggcaggaccc gtccccggag ggtgatctcc
     3901 ccggtcatgg ccaagtccga tcgtacggga atgcgggtga gggcggatac cagggaggtg
     3961 gccatggtga tgcccgccga cggaccgtcc ttggggatgg ctccttcggg cacatggacg
     4021 tggatgtcga atttctggta gaaatccggc tcgatgccca ggcgcgccga ccgggacctc
     4081 acgtagctca gggccgcctg ggcggattcc tgcatcactt cccccagttt ccccgtaatg
     4141 atcatcttcc ctttgccggg catgatgatg gattcgattc ccaggatatc cccgccgaat
     4201 tccgtccagg ccagcccgat ggccaggccc acctcgtcct tttcttcggt tctcccgtac
     4261 cggtacttgg gaattcccag atactcctgg accagctcct cggtgagctc gatccgggtt
     4321 tccggcccct gccgcaccac ttccttggcc actttgcggc acacggacgc gatttcccgc
     4381 tcgaggtttc ggacccccgc ttccttggtg tagtggcgaa tcatgtacag gaggatctcg
     4441 tcggtgaaga tcacgtttcc cggctgcagc ccgttggcct tgcactgctt ggagaccagg
     4501 aaattccggg cgatgttcag cttgtcgaat tccgtgtatc cggcaatctg gatgatttcc
     4561 atccggtccc tcaggggagg cggaatggaa tggaggttgt tggcggtagt gatgaagaag
     4621 acctcggaaa ggtcgtaatc caggtccagg tagtgatcgt tgaaggcgaa attctgttcg
     4681 ggatccaaca cctcaagcag ggccgccgag ggatctcccc gaaagtccat gctcatcttg
     4741 tcgatctcgt ccaggcagaa gaccgggttg ttgctcttca cctttcgcag gctctggatg
     4801 atctttccgg gcagcgcccc gatgtaggtt ctccggtggc cgcggatctc cgcctcgtcc
     4861 cgcacgcctc ccagggagag ccgaatgaag ttgcggttca tggagcgggc aatggaacgg
     4921 gcaagggagg tctttcccac cccgggggga ccgacaaaac acaggatggg tcccttgatc
     4981 ttcttgacca gggcctgcac ggccaggtat tccaggatcc gttccttggg cttctcgagg
     5041 ccgtagtgat cttcctcgag gatgaccgat gccccttcga tgtccagctt gtcccgggtc
     5101 ttctcgtacc agggcaggga gaggatccag tcgatgtagt tgcgaaccac cgtggcctct
     5161 gcgctcatgg gagacatcag cttgagcttc ttaagctcgg cccggacttt ggtggcggct
     5221 tcctggctca attttttgcg cttgatgcgc ttctcgagct cctcgagctc ggacttgaag
     5281 tcgtcctttt cccccatttc cttctggatg gcgcgcatct gttccgtgag gtagtattcc
     5341 ctctgggtct tttccatctg ctttttcacg cgacccttga tgcgctcttc ggtgagcccg
     5401 atttcgattt ccgcccgcag gtgcccgaaa accttctcca tcctctgggc cacatccacg
     5461 gtctccaaga tttgctgctt gatgtccagt ttgaagggga cgtaggacgc gatggtatcg
     5521 gtcagccggg agggttcgtc gatggccgcg acggcgtcca aaatctcctg ggtgattttc
     5581 ttgttgtgtt tggcgtaggc ttcgaaggtg gagcgaatcc cgcggcgcag ggcttccacc
     5641 tggatggggt tgggcggagg ttcggagagc acctccacct gcacgtggaa gtagtccggg
     5701 tggggaacga agtggagcac ccgggcccgc tgttctcctt ccaccagcac cttgaccgtt
     5761 ccgtcgggca accgaaggat ctgtaggatg ttggcaatgg tcccgatacg gtagatgtcc
     5821 gattcgccgg gggtatcggt ctttgccttg gtctgggcgg ccagaaagat ccgtttctcg
     5881 gaactcatgg ccttatccag ggcattgacc gacttatccc tgccgacgaa caggggaacc
     5941 acgatggagg gaaacaccac gatgtccctg aggggcaaca gcggaagaga aacggggctg
     6001 acagggttct gctgatcctc tttgtttcgg tttagcttga acatggcacg acactcttct
     6061 tccttggtat cgtataggat gtagccatct gaagtcgtga cttcgaggtg tccgcgacgg
     6121 gggcgtcagg gccgcatcct caaccctgcc ttcccgttac gcagactctt gattctggcc
     6181 tcggtagagc agaagcgggg tctcgtgctt caggatgacg tcttctccga taatgcactc
     6241 ctggatctcg gggtgggagg gcagatcgta catgatgtcc agcatgatgt tttccatgat
     6301 ggaccgaagg ccccgggccc cggactttct tcgcagcgct tccttggcga tggcccgaag
     6361 cgccccttcg gtgaaccgaa gacgaacctc ctcgagctcg aagagcttct tgtattgctt
     6421 caccaaggca ttcttgggtt ccgtcaggat cctcaccaat tcatcttcgg tgagttcgtt
     6481 caaggtggcg atgaccggca tgcggcctac aaattccggg atcatgccga attttatgag
     6541 gtcctcgggc tgaacctgga tgaggatttc gccgatattg cggtcttcct tgctgcgtat
     6601 ttcggcgccg aaccccatcc ctttgactcc gacccgggac tggatgatgg tttcaaggta
     6661 gttgaacgct cccccgcaga tgaaaaggat gttggtggtg tcgatcttga tgaattcctg
     6721 ctgggggtgt ttccttcccc ctttgggggg aacattggcc accgtcccct cgaggatctt
     6781 gaggagggcc tgttggacac cctccccgga aacgtcccgg gtgatggacg ggctgtcgga
     6841 tttcttggcg atcttgtcga tttcgtcgat gtagacgatt ccccg
//
LOCUS       DAOOVC010000187         6850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6546_length_6850_cov_5.910228, whole genome shotgun sequence.
ACCESSION   DAOOVC010000187 DAOOVC010000000
VERSION     DAOOVC010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6546_length_6850_cov_5.910228"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLM79_16230"
     CDS             complement(<1..374)
                     /locus_tag="PLM79_16230"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV87905.1"
                     /translation="MADMHETICRCLSEQANQGIFATDGGLTITSWNRWLELHSGRTT
                     SQMVGCGLLEAYPDLVDRGLDRVYQEALSGRSVMLSQRFHRYLLPMPSAVHDMETASA
                     PHMLQSARISPLTENGRVVGTLT"
     gene            complement(415..1017)
                     /locus_tag="PLM79_16235"
     CDS             complement(415..1017)
                     /locus_tag="PLM79_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87906.1"
                     /translation="MILTEEQKDSLVEVINIAFGRAAGSLSTITGQRVLLSVPNVSVC
                     PIGELHSNLEAFIQGDVATVHQIFSGPIAGSALLVLDYKGAVLLSGLLTEGRVPIERL
                     DASDREVLTEVGNILLNACLGTFGNLLKVHISFSVPRLHVEALGGLLNSLTIDSKELQ
                     IALMVLTNFQLRDSAVGGYLVIVLGVSSLDQLVESIGKME"
     gene            complement(1167..1529)
                     /locus_tag="PLM79_16240"
     CDS             complement(1167..1529)
                     /locus_tag="PLM79_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87907.1"
                     /translation="MARILIVDDSNMARRMLRKILESGGHEVLDEPDGLAGLERFALE
                     KPDLVFLDLSMPGLSGLDVLEKMRRMDPGVRVLIATADVQKLTREVALAGGAAGYIIK
                     PFEKDPVLDAVSAALKEA"
     gene            complement(1559..2605)
                     /locus_tag="PLM79_16245"
     CDS             complement(1559..2605)
                     /locus_tag="PLM79_16245"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOV87908.1"
                     /translation="MDRVMRVLVVDDSAYVRKVVKQMLSKSPFLEVVGTARDGEEALD
                     LVKELRPDVVTLDLIMPNMDGVEFLRRQMTLRPLPVVVVSIASESGEMALAALDAGAV
                     DFVQKPTALATDKIYEISNELVEKVKTAASVSVSRLKFAPTDREKPTVLAAPVRTRGT
                     VDIVVIGISTGGPQALKYLIPRLPADFPVPVAVVLHMPIGYTEMYARSLDAVSPLKVV
                     EAAGDEPVRPGVVFIAPAGRHLKFQGNRERGVTTVLDARPFDTPHRPAVDVLFESAAQ
                     AFGERVLGVVMTGMGADGRAGAAWIKAQGGLVFTEAEESCVVYGMPRSVAEAGLSDRV
                     VALDRMAQAILETI"
     gene            complement(2718..3098)
                     /locus_tag="PLM79_16250"
     CDS             complement(2718..3098)
                     /locus_tag="PLM79_16250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOV87909.1"
                     /translation="MKEEDRFSHKIIGAAIEVHRVLGPGLLESAYEECLCRELSIQEI
                     PYERQKPLPICYKTIVLDCGYRLDLVVAGKVIVELKAVERLDAIHEAQLLTYLKLSSL
                     KLGILINFNVPVLKQGIKRMVNSL"
     gene            complement(3142..3987)
                     /locus_tag="PLM79_16255"
     CDS             complement(3142..3987)
                     /locus_tag="PLM79_16255"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOV87910.1"
                     /translation="MRFYYETLSLSDSEFTILRDLVHERIGLFYDNGKREQLADKLSP
                     LVLESGLSSFLDFYYLLRYDDVNAGPQWERVADALSVQETYFWREMDQVRALVDVLAP
                     AHFASNTCRPLRIWSAGCATGEEPLTIAMALDEAGWLRRGNIEIHASDMSPAAIEKAR
                     KGLYRERSFRNLPPRLKTRYFVSHEGCWQVDPELHSRIRWSVINLRAHREAVSRIPEM
                     SVIFCRNVFIYFSEAGVRSTVRLFYDRLQTPGYLCVGAAESLLKATGDLELEEIGGAF
                     VYVKR"
     gene            complement(4096..>6850)
                     /locus_tag="PLM79_16260"
     CDS             complement(4096..>6850)
                     /locus_tag="PLM79_16260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOV87911.1"
                     /translation="HNVCNNQTAMPEGSATASEACGKPLADLLPDLQPRGLLDRFQGV
                     LEQGTVEVLAPAFHHHLVPCTPEAPSKRFTRMRQRVTLAPLRAEERIVGVLVTIEDVT
                     ARMDRERDLAEQLSSPDEQTRLQAAEALAADESLEPEHTLIGALNDPSWRVRRVAVRG
                     IAGHGGDRAVPALLRALRHEYNNMTVLNSALQVLAMMNLDTVGPLIEFLGDPDTELRI
                     YAASALGETGDLEAAPALIRALDDADPNVRFHAIEALGKLGARDAVEQLCALAESGDF
                     FLAFPALDALRRIGDPAAAPRIVSLLEDGMLREAAVEALGELGGEESVAPLAGLLNSH
                     DGPFRLTASALASLYHRYESRYGEGAHVADLGRRSITPSGAWNLIETLNDPEVQGDTK
                     TLRAVALVLGWLEGPVVQRSLTRLLGQPTVRREVVEALVRHGNRVTDLLIEQLVSEDL
                     DTRKAAAIALGRIGDAGSVAPLIDALEQDEALIGVAAGALAKIGDRSAFEALLGFLGH
                     PDVTVRQSIIAALNSLGHPDMSRRMASLLQDPSPTVRESAVKIAGYFGYPECLDLLFE
                     RCVDPSETVRCAAIEHIAYLEDDRAAPALAEALTDPNPRVRAASARALAHVEGAKSLP
                     HLLTALGDTDPWVRYFSARSMGKFEKLDCGHSVSRGGAEDPSCLRWPLGHEIIEKLIH
                     LVLKDPAIQVRIAAMEALGNVAGARAVPVLSPLTEDANPDIARAAMGALGLIGHPEAL
                     KPILAGLHTSDPVRRQHAIRALGERGGLDAAGALQWLAATDPDDRVVEAAMEALGHMG
                     SSEAVTALLALAEDPRCREACVSALASLGEAKADWVARGLDHAHAEVRRATVQTLARM
                     KRPGATERLLAALEDREPAVRLEAVGALGLLGSRQGRTKLAELARTDPDLGVRAAARK
                     MLG"
BASE COUNT         1370 a         2136 c         2028 g         1316 t
ORIGIN      
        1 gttagggtgc ctaccacgcg gccgttttca gtcaggggag aaatccgcgc ggactggagc
       61 atgtggggcg cagaggccgt ttccatgtcg tggacggcag agggcatggg aagcaggtag
      121 cggtggaacc tctgcgaaag cataacactc cggccggaca gcgcttcctg gtaaacgcga
      181 tccagcccgc gatccacaag atcggggtag gcttccagga ggccgcaccc caccatttgg
      241 gacgttgttc ggccgctgtg cagctccagc cagcgattcc aactggtgat ggtaagcccg
      301 ccatcggtgg cgaaaatgcc ctggttggcc tgctcgctca gacagcggca aatggtttcg
      361 tgcatgtcgg ccatgtttct ctgtgctctc cgtgtcctcc gtggtgaaat gcttctattc
      421 catcttccct atggattcca ccaattgatc cagggaagaa acgcccagca cgatgacaag
      481 atatccaccc acggcgctgt cccgcagttg gaagttcgtc agcaccataa gggcgatctg
      541 gagctccttg gagtctatgg tgagggagtt gagcagaccg cccaaggcct cgacgtgcag
      601 ccgggggacc gaaaaggata tgtgcacctt caaaagattg ccgaaggtcc ccaggcaggc
      661 gttgagcaga atgttgccga cttccgtcaa gacctctcga tccgaagcgt ccagccgttc
      721 gatggggacg cggccttcgg tcaagagccc tgaaaggagg acggcgcctt tgtagtcaag
      781 cacaaggaga gcgctgccgg caatggggcc ggaaaagatc tggtggaccg tcgccacatc
      841 gccttgaatg aaggcctcca ggttgctgtg cagctctccg atggggcata cgctgacgtt
      901 gggcaccgaa agcagcacac gctgaccggt aatggtcgaa agcgaacccg cggcgcgtcc
      961 gaaagcgata ttgatgacct cgaccagtga atccttctgt tcttctgtca gtatcatttc
     1021 gtggctcgct cctttgcgat atggtgaggg gatgaattat taaccacaga ggacacagag
     1081 aacacagaga gaacgaaaaa ggagaggtct tttctctttt ttgtctccgt gccctctgtg
     1141 ctctctgtgg ttaaatctta caaaacctac gcctccttca gggccgcgct caccgcatcc
     1201 agcaccgggt ccttctcgaa gggcttgatg atatacccgg cggccccgcc ggccagggcg
     1261 acctctcgag tgagcttctg aacgtccgcc gtggcgatca ggacccggac accggggtcc
     1321 atgcggcgca tcttctcgag cacatcgaga cccgagagac cgggcatgga gagatccagg
     1381 aataccaggt cgggcttctc cagggcaaaa cgctcgagcc ccgcaagtcc atccggctca
     1441 tccaggacct catgccctcc cgactcgagg atcttgcgca acatgcgccg cgccatgttg
     1501 gaatcatcga caatgagaat tctggccatt catttgtcct ccacgtagtt catcgatgtt
     1561 atatcgtttc cagaatcgcc tgcgccatcc ggtccagggc aaccacccgg tcgctcaagc
     1621 ccgcttcggc taccgaccgg ggcatgccgt aaaccacgca cgattcttcc gcttcggtga
     1681 ataccaggcc gccctgagcc ttgatccagg cggcgcccgc tcggccgtcc gcgcccatgc
     1741 ccgtcatcac tactccaagc accctctcgc cgaaggcctg agcggcggat tcgaagagca
     1801 cgtccaccgc cggccggtgg ggggtatcga agggccgggc atcgaggacg gtcgttactc
     1861 cgcgctcccg gtttccctgg aacttgagat gccgtcccgc gggggcgata aacacgactc
     1921 cgggacggac cggctcatct ccggcagcct ccacaacctt gagcggggag acggcgtcga
     1981 ggcttctggc gtacatctcc gtatatccga tgggcatatg gagcaccacg gccacgggta
     2041 ccgggaagtc ggcgggaagc cgcggtatga ggtacttgag tgcctgggga cctcccgtcg
     2101 agatgccgat caccacgata tcaacggtgc cccgcgttct cacgggcgcg gcgagcacgg
     2161 tcggcttctc ccggtccgtc ggggcaaact tgaggcgtga cacggaaaca cttgcggcgg
     2221 tcttcacttt ctccaccagc tcattgctga tctcatagat cttgtccgtt gcgagggccg
     2281 taggcttctg cacaaagtcg acggcgccgg catcgagggc cgccagggcc atctctccgc
     2341 tttcactggc tatgctcacc acgacgacgg gcagcgggcg aagcgtcatc tgacgcctca
     2401 ggaattccac gccgtccatg ttgggcatga tgagatcaag cgtcacgaca tcgggccgga
     2461 gctccttcac aaggtcgagg gcctcctcgc cgtcccgcgc cgttcctacc acctcgagaa
     2521 aagggctctt cgacagcatc tgtttcacga ccttgcgcac ataggccgaa tcatccacca
     2581 ccaacacgcg catcacacga tccatccttt tctccttgat gaaaagagca tcaattcacc
     2641 gcaaagacgc aaagagcgca aagtctttcc aatacaaatt ccgtctaaat ccttgcagtg
     2701 atatggcttc cccttcttta caaactgttc accatgcgct tgatgccttg tttcagcaca
     2761 ggcacgttga aattgatcaa gattcccagt ttgagagaag acagcttcag ataagtcaac
     2821 aactgagcct cgtgaatggc atcaaggcgt tccaccgctt tcaactcgac gatgaccttg
     2881 cctgccacga ccaggtccaa gcggtatccg caatccaata cgatggtttt gtagcaaatg
     2941 gggagcggct tctggcgttc atagggtatt tcctgaatcg acagttcacg gcatagacac
     3001 tcctcatagg ccgactcgag caaaccgggg ccaagaacgc gatgcacctc gatagccgca
     3061 ccaatgatct tgtgtgaaaa cctgtcctct tctttcattc atttctttct ttgcgctctt
     3121 tgcgcctttg cggtgaatgc ttcagcgttt gacgtatacg aaggccccgc ctatctcttc
     3181 caactccagg tcgccggtcg ccttgagaag cgattccgcc gctccgacgc aaagatatcc
     3241 cggtgtctgc agccggtcgt aaaaaagccg caccgtgctt cgaacacccg cttctgaaaa
     3301 atagatgaaa acattgcggc agaagatcac cgacatctcg ggaatgcggg aaaccgcttc
     3361 ccgatgagcc ctcaagttga tgaccgacca ccggattcgg gaatggagct ccgggtccac
     3421 ctgccagcac ccttcgtgcg acacgaaata tctggtctta agccgagggg ggagatttcg
     3481 aaaagagcgc tcccggtaaa gcccctttcg agctttttct atggccgccg ggctcatgtc
     3541 gcttgcatga atttcgatat tcccccgccg aagccatcca gcctcatcga gggccattgc
     3601 gatggtaagg ggctcctctc ccgtggcgca ccccgcgctc cagatccgca gcggtcggca
     3661 cgtgttggag gcgaaatggg cgggggccaa aacgtccacc agggcgcgca cctggtccat
     3721 ctctctccag aagtacgttt cctgcaccga cagggcgtcg gccacccgct cccattgagg
     3781 cccggcattc acatcgtcgt aacggagaag gtagtagaaa tccaaaaagg agctcaaccc
     3841 gctttcgagg acaagggggg aaagtttatc ggccaactgc tcgcgcttgc cgttgtcgta
     3901 gaaaagcccg attcgttcgt gaacgaggtc acgtagaatc gtgaactcac tatcggaaag
     3961 gctgagtgtt tcgtagtaaa agcgcattgt tacatccgta ctaatttact caccgcaaag
     4021 gcgcaaagga cgcaaagaaa aacctctttt ataaaacctt tgcgctcttt gcgtctttgc
     4081 ggtgaatgtc aaatttcagc caagcatctt ccttgccgcg gctcgtacac cgagatccgg
     4141 gtccgtcctt gccagctccg cgagctttgt ccggccctgc cggcttccca ggagaccgag
     4201 tgcgccgaca gcttcgagcc tcaccgcggg ctcccgatcc tccagggccg caaggagcct
     4261 ttccgtggct cccggccgct tcatgcgggc cagagtttgc actgttgccc ttcggacctc
     4321 cgcatgggca tggtccagcc cccgagcgac ccagtccgct ttggcctcac ccaaggaagc
     4381 gagggcgctc acacaggcct cccggcacct cgggtcctcg gccagggcca ggagggccgt
     4441 taccgcctcg gagctcccca tgtggccaag ggcttccatg gccgcctcca cgacccggtc
     4501 gtccgggtcg gttgccgcca accactggag tgcaccggcg gcgtcgaggc cgcctcgctc
     4561 gcccaaggca cggatagcgt gttggcgcct gacggggtcg gaggtgtgaa gccccgccag
     4621 gatcggcttg agggcctcgg gatggccgat gagcccgagc gcccccatgg cggcgcgtgc
     4681 aatgtccggg ttggcgtcct cggtgagggg agatagaacg ggaacggccc tggctcccgc
     4741 gacgtttccc agggcttcca tggccgcgat gcggacctgg atggccggat ctttcagaac
     4801 gaggtggatg agcttctcga tgatttcgtg gccgagcggc catcggaggc acgatggatc
     4861 ctcggcgccg ccgcgggaaa cggaatgacc gcagtccagt ttttcgaact tgcccatgga
     4921 gcgggcagag aagtagcgca cccacgggtc cgtgtcgcca agcgccgtga gcagatgggg
     4981 cagggactta gccccttcca cgtgtgccag ggcgcgggcc gatgcggccc gcacgcgcgg
     5041 gttggggtcc gtgagcgctt cggcaagggc cggtgccgcc cggtcatcct caaggtacgc
     5101 gatatgctcg atggctgcgc aacgaaccgt ttcggaagga tcgacacacc gctcaaaaag
     5161 gagatccagg cattcgggat agccgaaata acctgcaatc ttcacggccg attcccgcac
     5221 tgtcgggctc gggtcttgga gcagagaggc catgcgccgg gacatgtcgg ggtggcccag
     5281 ggaattgaga gcggcgatga tcgactgacg cacggtgaca tccggatgcc caagaaagcc
     5341 cagaagggcc tcgaaagcac ttcggtcgcc gattttggcc aacgctccgg cggcaacacc
     5401 gatgagggcc tcgtcctgtt cgagggcatc gatgaggggc gcgaccgacc cggcatctcc
     5461 tattcgcccc agggcgatgg cggctgcttt gcgggtatcg aggtcctcgg agacgagctg
     5521 ctcgatgagc aggtccgtca ctcgattgcc gtggcgcacc agggcttcca ccacttcccg
     5581 ccgaaccgtg ggctgaccga gaaggcgggt gagggaacgc tggacgacag gaccttcgag
     5641 ccagccaagg acgagggcaa cggcccgcag cgtcttcgta tctccttgta cctcgggatc
     5701 gttgagcgtc tctataaggt tccaggcccc ggatggagtg atcgaacggc gccccaggtc
     5761 cgcaacatgg gcgccttctc cgtaccggct ttcgtagcgg tgatacaggg acgccagggc
     5821 gcttgccgtc aggcggaaag gtccgtcgtg actgttcaac agcccggcca ggggcgccac
     5881 cgactcttca ccgcccagtt caccgagggc ttccaccgcc gcctcacgca gcatgccgtc
     5941 ttcgagcaga ctcacgatgc gcggggcggc cgccgggtcg ccgattcggc gcagggcatc
     6001 cagggcggga aaagccagga agaagtcgcc cgattcggcc aaggcgcaga gctgctccac
     6061 ggcatcccgt gcccccagct ttcccaaagc ctcgatggca tgaaagcgca cgttggggtc
     6121 ggcatcgtcc agggcacgga tcaatgccgg cgcagcctcc aaatccccag tctctcccaa
     6181 ggccgatgct gcatagatgc gcaattccgt gtcaggatcg cccaggaatt caatcaacgg
     6241 tccaaccgta tccagattca tcatggccag gacctggaga gcgctgttca aaacagtcat
     6301 attgttgtac tcgtgacgca gcgcccgcag caatgcgggc accgcacgat ctccaccatg
     6361 cccggctatt ccccgcacgg caacgcggcg cactcgccaa ctggggtcgt tgagggcgcc
     6421 aatgagggta tgctcgggct ccaggctttc atccgcggcg agagcttcag cggcctgcaa
     6481 acgggtctgt tcgtcgggac tcgaaagctg ctcggcaagg tcccgctcgc gatccatccg
     6541 cgccgtcaca tcctcgatgg taaccaggac gccgacgatg cgctcttccg cacgcagcgg
     6601 ggccagagtg acccgttgcc tcattcgggt gaagcgcttc gacggagctt ctggggtgca
     6661 ggggaccagg tgatggtgaa atgcgggcgc caggacttcc accgttccct gctccaggac
     6721 tccctggaac cggtcgagaa gccccctggg ttgcaaatcg ggcagcagat ccgcaagagg
     6781 ttttccgcaa gcttctgagg ctgtcgcgga accctccggc atggctgttt gattattaca
     6841 gacgttatgg
//
LOCUS       DAOOVC010000188         6827 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6572_length_6827_cov_6.853957, whole genome shotgun sequence.
ACCESSION   DAOOVC010000188 DAOOVC010000000
VERSION     DAOOVC010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_6827_cov_6.853957"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLM79_16265"
     CDS             complement(<1..345)
                     /locus_tag="PLM79_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87912.1"
                     /translation="MTRNPTPASTLQSPPFEPLEEEARECPGVMGIDVGSRSIELAVL
                     EKGRWKEVRKLPTTFDPLGQCRKLLWDAAGYAVVATGYGRKLVADSFPGLEVTTITEI
                     QAYALGARHLFPQ"
     gene            complement(367..1641)
                     /locus_tag="PLM79_16270"
     CDS             complement(367..1641)
                     /locus_tag="PLM79_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HOV87913.1"
                     /translation="MSTPYDAMWEKLNLDLDAHAGLLEVLGKFYGDIYLSQAGRLKGM
                     EYLDFVLSEVHGLRIQELLQAKAQGRKVIGTFCVFVPEEIVLAADAVQVGLCAGAEAG
                     KEAAEKILPRNTCALIKSFVGFKLSRLCPFIESCDLVVGETTCDGKKKAYEIFEEYVP
                     VYVMEIPQMKQKCDRDLWRTEVLRFKEKVEEVTGRTITAEKLGNAVRLVNDRRRTLQR
                     LDRLRAADPVPISGRDVLLINQVSFYDDPVRFTQSVGKLCDELEERVKAGEGVVPPGT
                     PRLMLSGCPMAVPNWKLPYIIESSGAVIVGEESCIGRRNTRDLVTENGGKTDDLIDAV
                     VDRSMKIDCACFTPNRERLDHIVDMARELKVDGVVHYGLSFCQPYAIEAFKVEKSLRD
                     AGIPMLSIETDYSMEDVHQLKTRVEAFVEMVR"
     gene            complement(2026..3375)
                     /locus_tag="PLM79_16275"
     CDS             complement(2026..3375)
                     /locus_tag="PLM79_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOV87914.1"
                     /translation="MVEKVIIVGAAGRDFHNFQVYFKDNPRYRVVAFTAAQIPRIEGR
                     VFPAELAGELYPRGIPIHPESEMVRIIRENRADLVAFSYSDVSHLDVMHKASVAMSEG
                     ADFILIGATYTMLKAKKPVVAVCAVRTGCGKSQTTRKVCSILRKWCRRVVVVRHPMPY
                     GDLRLQAVQRFSSYEDFDRNHCTIEEREEYEPLVDRGIVVYAGVDYGRILEKAQEEAD
                     VLVWDGGNNDTPFYFPDLHIVLFDPHRPGHELRYYPGETNMLMADVAIINKVDTASEE
                     GVRQVEENIRRHNPKARIVYAESPITVEDPARIFGKRVLVVEDGPTLTHGEMPFGAAT
                     IAAQRSGAGSIADARPHAVGSIREAYRKYPHLGPQLPALGYGEEQIRELEATIRAVDC
                     DLVLFGTPTDLSRLLRIDKPVLRARYEYRDHGPPLLEDILEEDLNRLGLPESESERR"
     gene            complement(3407..4099)
                     /gene="aat"
                     /locus_tag="PLM79_16280"
     CDS             complement(3407..4099)
                     /gene="aat"
                     /locus_tag="PLM79_16280"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOV87915.1"
                     /translation="MPVYRLAKPLVFPSPSLAEPDGLLAVGGDLSPQRLLLAYRQGIF
                     PWYSENTPILWWSPDPRLVLIPGELTVSRSLVRVLRKSLFRVSFDRAFRRVVESCAVV
                     PRRYGDGTWIVPEMMDAYEELHRMGYAHSVETWQNGVLVGGLYGVALGRAFFGESMFA
                     LRPDASKVALVHLVRLLIRWGFDLIDCQVVTEHLRRLGAREVPRQQFLSQLEAAVAKK
                     SVRGSWASVVLG"
     gene            complement(4123..6450)
                     /gene="clpA"
                     /locus_tag="PLM79_16285"
     CDS             complement(4123..6450)
                     /gene="clpA"
                     /locus_tag="PLM79_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HOV87916.1"
                     /translation="MINRELELTFSAAVKEAKSRRHEFLTLEHILFALLHDLTGRKIL
                     FHCGADLDELKTRLEKFFAERVEKLPEGTQHDPMQTIGVQRVLQRAIMHVHGAEKKEV
                     DAGDVLAAMFYEESSYAVYFLKSQGVSRLDVLNYISHGISKVGDPGMEEEPGQAIQPR
                     LQGAAQETRRASALESFTVNLIRKAADGLIDPLIAREEEILRTLQILGRRTKNNPIFV
                     GDPGVGKTAIAEGLALKIQRGEVPDAFKNIEIYALDMGAVLAGTKFRGDFEARLKAIL
                     QELKRKPGVILFIDEIHTVVGAGATSGGSMDASNILKPVLVTGGLRCIGSTTYEEYKN
                     HFEKDRALSRRFQKVEIHEPSVEETYKILLGLRSYYEKHHGVRYTDAALRAAAQLGAR
                     HINDRYLPDKAIDVIDEAGAALRLKKDRRVRRIVGPKDIEQVVARIAKIPARSVSSSD
                     QLRLHSLENELRSQVFGQQAAIEALAKAIKRSRAGLRVPDKPIGSFLLIGPTGVGKTE
                     VAKQLAQILGVHFLRFDMSEYMEKHTVSRLIGAPPGYIGFDQGGLLTDAIRKYPYTVL
                     LLDEIEKAHPDIFGILLQVMDHASLTDNNGKKADFRNVILLMTSNAGAREMSSPSIGF
                     GAQEGDSRVAKGMKAVENLFSPEFRNRLDGIVAFNPLSPQIMERIVDKFVRELETQLS
                     EKKIRLELSPAARSWLAEKGHDPTFGARPLARLIQAEIKDVLADEILFGRLKQGGKVF
                     IRRADESGSESEGVLKTESLAFRFFDTPVSETGGG"
     gene            complement(6451..>6827)
                     /gene="clpS"
                     /locus_tag="PLM79_16290"
     CDS             complement(6451..>6827)
                     /gene="clpS"
                     /locus_tag="PLM79_16290"
                     /inference="COORDINATES: protein motif:HMM:NF000672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HOV87917.1"
                     /translation="NLRQGKDSNHGSTFFPGGAAMGDRTPDFREDLKDRVEEELDEPP
                     MYRVLLHNDDYTTMEFVVEVLQQVFHKSPSEATRIMLLVHKSGTGLCGVYTEEIAETK
                     VALVHHLARKNGYPLQCSMEEA"
BASE COUNT         1303 a         2082 c         2050 g         1392 t
ORIGIN      
        1 ctggggaaac aaatggcgag cccccagggc gtaggcctgg atttccgtga tggtggtcac
       61 ctccaggccg ggaaaagaat ccgcgaccag tttgcgcccg tagcccgtcg ccaccacggc
      121 ataccctgcg gcgtcccata gaagcttccg acattggccc aggggatcaa aggtggtcgg
      181 cagtttccgg acctccttcc atcggccctt ctccaacacc gcaagctcga tggatcggga
      241 ccccacatcg attcccatca cccccggaca ttcgcgggct tcttcctcca acggttcgaa
      301 gggagggctc tgcagggtcg atgcaggtgt gggatttcgt gtcatgattc tgcgcccccg
      361 gccctgctac cggaccattt ccacaaaggc ctcgacccgg gtcttcagct ggtgaacatc
      421 ctccatgctg taatcggttt cgatggaaag catgggaatc ccggcgtccc ggagggattt
      481 ctcgaccttg aaagcttcga tggcgtaagg ctggcagaag gaaaggccgt agtgaacgac
      541 gccgtccacc ttcagttccc gggccatgtc cacgatgtgg tcgaggcgtt cccggttggg
      601 ggtgaaacag gcgcagtcga tcttcatgct gcgatcgacc acggcatcga tcaggtcatc
      661 cgtcttcccg ccgttttccg tgaccaaatc ccgggtgttt cgccgaccga tgcaggactc
      721 ttcccccacg attaccgccc ccgaagactc gatgatgtag ggcagcttcc agttgggaac
      781 cgccatggga cacccggaga gcatcaaacg gggggtcccg ggaggcacca caccctcccc
      841 cgctttcacc cgttcctcca gttcatcgca gagtttgccg acgctctggg tgaatcgtac
      901 aggatcgtcg tagaagctga cctgattgat gagaagaaca tccctgcccg agatgggaac
      961 cgggtcggcg gcccggagcc tgtccagtcg ctgcagcgtt cttcgccgat cattgaccaa
     1021 tcggacggcg ttccccagct tttccgccgt gatggttctc cccgtcacct cctccacttt
     1081 ttccttgaac cgcaggacct cggtgcgcca gaggtcccgg tcgcacttct gcttcatctg
     1141 gggaatttcc atgacataca cgggcacata ttcctcgaag atctcgtagg ctttcttctt
     1201 gccgtcacag gtggtttccc ccaccacgag atcgcaggat tcgatgaaag gacagagcct
     1261 cgaaagcttg aagcccacga aggatttgat cagggcacac gtgtttctgg ggaggatctt
     1321 ttccgccgcc tccttgcccg cttccgctcc ggcacacaga cccacctgga cggcgtcggc
     1381 cgcaagaacg atctcttcgg gcacgaagac acagaacgtg ccgatcacct tcctgccttg
     1441 ggccttggcc tgcaggagtt cctggatgcg aagcccgtgg acctcggaga ggacgaaatc
     1501 caggtattcc atccccttga gccgaccggc ctgggaaagg tagatatccc cgtagaattt
     1561 gcccaagact tccagcaatc cggcatgggc gtccaggtcc aggttgagtt tctcccacat
     1621 ggcatcatag ggcgtggaca tgggtctgac ctccttggaa aggaccgatg ggtctgcatg
     1681 gctgatcaca attcagtccc cccgcctctc cggactcgtc ctccccgggt caccccggcg
     1741 ggatgagggt cccgagcccc gggagagcag ggtcccgggg cggctccgca ccgacgcggg
     1801 gttcgacgca gcgacgatga agggaatgac ggggagtact cgggacagtt tcttataatc
     1861 gggccgtttc aatcctacca atcgataatc cggatgctcc gaagcccaag cattgggaag
     1921 gacgatgaaa aaggtccgga aaggacgacg gaccccgggg aagaaggatg aaccggggga
     1981 ccagggggga tgcgcaggga ctgtcctggg gaaccggcaa ggctcctaac ggcgctccga
     2041 ttcgctctcg ggaaggccca gcctgttcag atcttcttct aagatgtctt ccaggagagg
     2101 aggtccgtga tcccggtatt cataacgggc acgaaggacg ggcttgtcga tgcgaagcag
     2161 gcgcgacagg tccgtgggag ttccaaacag gaccaggtcg cagtctacgg cgcggatggt
     2221 ggcttccagc tcccggatct gctcctcgcc gtatcccagg gccggcagct ggggacccag
     2281 gtgggggtac ttgcggtagg cttcgcgaat gcttcccacc gcgtggggac gggcatccgc
     2341 gatggaaccc gccccagatc tttgggccgc aatggtggcg gctccgaaag gcatctcccc
     2401 gtgggtcagg gtgggaccgt cttccactac caggacccgc ttgccgaaaa ttcgcgccgg
     2461 gtcctccacg gtgatgggag actccgcata cacgattcgg gctttggggt tgtgtcgccg
     2521 gatattttcc tccacctgtc gaaccccttc ctcggaagcc gtatccacct tgttgatgat
     2581 ggcgacatcc gccatgagca tattggtttc ccccgggtaa taccggagct catggccggg
     2641 gcgatgggga tcgaacagga cgatgtgaag atccggaaaa tagaaaggcg tgtcgttgtt
     2701 tcccccgtcc cagacgagaa catcggcttc ttcctgggcc ttctcgagga ttcgaccgta
     2761 atccactccc gcgtagacca caatgccccg gtccaccagg ggttcgtact cctctcgctc
     2821 ttcaatggtg caatgattgc ggtcgaagtc ttcataggac gagaatcgct ggacggcctg
     2881 gagtcgaagg tccccgtagg gcatggggtg ccgcaccacc accacccttc ggcaccattt
     2941 ccggaggatg ctgcaaacct tgcgggtggt ttgggatttt ccgcacccgg tcctcacggc
     3001 acacaccgcc accacgggct ttttcgcctt gagcatggtg taggtggccc cgatgaggat
     3061 gaaatcggcg ccttcggaca tggccacgga ggctttgtgc atcacgtcca ggtgggacac
     3121 gtcgctgtag gagaaggcca ccaggtccgc ccggttttcc cggatgatcc gcaccatctc
     3181 cgactccggg tggatgggaa tcccccgggg atagagctcg cccgccagtt cggcgggaaa
     3241 aacgcgcccc tcgattcggg gaatctgggc cgcggtgaaa gcaacgaccc ggtagcgggg
     3301 attgtctttg aaatacacct ggaagttgtg gaaatcccgt ccggcggctc ccacgatgat
     3361 caccttttcg accatgtccc ctccccggaa gtggtggaaa tggaattcag cccaagacga
     3421 cggaggccca ggaccctcga acggacttct tggccacggc cgcttccaat tggctcagga
     3481 attgctgtcg gggaacctcc cgtgccccca gtcgtcgcaa gtgttcggtc accacctggc
     3541 aatcgatgag atcgaagccc catcggatga gcagtcggac caggtggacc agggcgacct
     3601 tggatgcgtc gggccggagg gcaaacatgg attccccgaa gaaggccctc cccaaggcca
     3661 cgccgtacaa accgcccact aagaccccgt tttgccaagt ttccacggaa tgggcgtaac
     3721 ccatccggtg gagctcttca taggcatcca tcatttcggg cacgatccag gtgccgtcgc
     3781 cgtagcgtcg cggcacgacg gcgcaggatt ccacaacccg ccgaaaggca cggtcgaagc
     3841 tcacccggaa gagggatttt ctcaggaccc tcaccaggct ccgactgacg gtcagctccc
     3901 cgggaatcag caccagccgg ggatcggggg accaccaaag gatgggagtg ttctcggaat
     3961 accaggggaa gatcccctgg cgataggcca gcaggagccg ctggggggac aagtcccccc
     4021 cgacggcaag aagaccgtcg ggctcagcca gcgacgggga cggaaacacc aatggttttg
     4081 ccaagcggta gacgggcatg agaactcata gaccggttgc gcctagcccc ccccggtctc
     4141 cgaaaccggg gtgtcaaaga agcgaaaggc aagggattcg gtcttcagga ccccctcgga
     4201 ctcggacccc gattcgtccg cccggcgaat gaagaccttt cccccttgct tgaggcgtcc
     4261 gaagaggatc tcgtcggcca gaacgtcctt gatttccgcc tggatcagtc gggccagggg
     4321 ccgcgctccg aaggtgggat cgtgtccttt ctcagccagc caggatcgag ccgccgggct
     4381 cagctccaac cgaattttct tttcagaaag ctgggtttcc agttcccgaa cgaacttgtc
     4441 cacgatccgt tccatgatct gggggctcag ggggttgaac gccacgatcc cgtccaggcg
     4501 gttgcggaac tctgggctga aaaggttctc caccgctttc atccccttgg ccaccctcga
     4561 atccccttcc tgggcgccga acccgatgga cgggctgctc atctcccggg ccccggcgtt
     4621 ggaagtcatg agtaagatga cgttacgaaa gtcggctttc tttccattgt tgtccgtgag
     4681 ggacgcatgg tccatgacct ggaggagaat gccgaagatg tccgggtgag ccttttcgat
     4741 ctcgtcaagc agcagaacgg tgtaagggta tttccgaatg gcatccgtga ggagtcctcc
     4801 ctgatcgaac ccgatgtagc cggggggagc accgatgagg cgggagaccg tgtgcttctc
     4861 catgtattcg ctcatgtcga agcgcaggaa atggaccccg agaatctggg ccagttgttt
     4921 agccacctcg gtttttccga ccccggtagg accgatgagg aggaaagacc cgatgggctt
     4981 gtcgggaacg cgcaacccgg cccgggatcg cttgatggct ttggccagag cctcgatggc
     5041 ggcctgctgc ccgaacacct gggaacgcaa ctcgttctcc aggctgtgaa gacggagctg
     5101 atccgaggac gaaacactgc gggcggggat cttggcaatg cgggccacca cctgttcgat
     5161 gtccttggga ccgacgattc ttcgcacccg ccgatccttt ttgaggcgca gggcggcccc
     5221 cgcctcgtcg atcacatcga tggccttgtc gggaaggtaa cggtcgttga tgtgccgggc
     5281 ccccagttgc gcggcggccc gcaaggccgc gtcggtgtac cggaccccgt gatgcttttc
     5341 gtagtaagac ctcaacccga gaaggatttt gtaggtctcc tccacggaag gctcgtggat
     5401 ttccaccttt tggaaccgtc ggctcagggc ccggtccttc tcgaagtggt tcttgtattc
     5461 ttcataggtg gtggaaccga tgcagcgcag tccgcccgtg accaggacgg gcttcagaat
     5521 gttggacgcg tccatggaac cgccgctggt ggcccccgcc cccaccaccg tgtgaatctc
     5581 gtcgatgaaa aggatgactc ccggctttcg tttcagctct tgaaggatgg ctttgagccg
     5641 agcctcgaag tcgcctcgga atttggtccc cgccaggacg gcccccatgt ccagggcata
     5701 gatctcgatg ttcttgaaag catccgggac ctcgccccgt tgaattttca gggcaagccc
     5761 ctcggcaatg gccgtctttc ccacccccgg gtctcccacg aagatgggat tgttcttggt
     5821 gcgccgcccc aggatctgga gggtccgaag gatttcctct tcccgggcaa tgaggggatc
     5881 gatgagccca tcggccgcct tccggatgag gttcaccgta aaggactcga gggccgaggc
     5941 ccttcgcgtc tcctgagccg caccctgcag gcggggctgg atcgcttgtc ccggttcttc
     6001 ttccatgccg gggtccccca ccttggatat gccgtgggaa atgtaattca acacatccag
     6061 ccgactcacc ccctgggact tgagaaaata cacggcatag gaactctcct cgtagaacat
     6121 ggccgcgaga acgtccccgg cgtccacctc ctttttctcc gctccgtgca cgtgcatgat
     6181 ggcccgctgg aggaccctct ggaccccgat ggtctgcatg ggatcgtgct gggtgccttc
     6241 ggggagcttc tcgacccttt cggcgaagaa cttctcgaga cgggttttga gctcgtccag
     6301 gtcggcaccg cagtggaaga ggatcttcct gcccgtgagg tcgtggagca gggcaaagag
     6361 gatgtgttcg agggtgagaa actcgtgacg ccggctcttg gcttccttga ccgccgctga
     6421 aaacgtcagt tccaattctc tgttgatcat ttacgcttct tccatgctac actggagagg
     6481 atatccgttt ttcctggcca ggtgatggac cagggcgact ttcgtttcgg cgatttcctc
     6541 ggtgtagact ccgcagagcc cggtgccgct cttgtgaacg aggagcatga ttcgggtagc
     6601 ttccgagggg gacttgtgaa acacctgctg gaggacttcc accacgaatt ccatggtggt
     6661 gtaatcgtcg ttatgcaata ggactcggta catgggaggt tcgtcgagct cctcctcgac
     6721 ccggtctttc aggtcttctc gaaaatcggg agtacgatct cccatcgctg ctcctcccgg
     6781 aaagaaggtg gaaccatggt tgctgtcttt cccttgtctc agattta
//
LOCUS       DAOOVC010000189         6727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6674_length_6727_cov_6.196343, whole genome shotgun sequence.
ACCESSION   DAOOVC010000189 DAOOVC010000000
VERSION     DAOOVC010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6727
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6674_length_6727_cov_6.196343"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="PLM79_16295"
     CDS             complement(<1..1584)
                     /locus_tag="PLM79_16295"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOV87918.1"
                     /translation="MQITCLGAARCVTGSCFLLDDEERYLIDCGLFQGGKQMEALNRK
                     EWGFRPEEISALFLTHAHIDHCGRIPKLVRDGFRGRIFCTLPTAELCKILLMDSAHIQ
                     EMEAEWQSRKRRRRGDPEMTPLYTLPDAEASFPFLETVPVGERIDLHPHLSFSFRNAG
                     HILGSSILELWVGPRDKPHKVVFSGDIGQKDQLIVRDPEAIGDADILFVESTYGNRNH
                     KTMEQSRRELVEAIHYSYNHGEKVIIPAFAVERTQEILYILGESFRKGLIPSMPVYLD
                     SPLAIAATEIFRRRSEFFDEDAMAILNHGQDPFDFPQLIFSRTAEDSIAINQKAGPAI
                     VIAGNGMCTAGRIKHHLKHNLWRKGCAVVIVGFQAEGTVGRKLVEGAPSVKVLGERIL
                     VRARVFTIGGFSAHADQQDLLDWIAHFGNPSLRVYAVHGEESISEVFASLVRQRYGFS
                     THVPGMGEVIPIPPTDRETAARQRQVPRWEQSWMRTLRKAEAVRAMGEKLPPRLLLQL
                     EEMLNRTERELDTVLSEAGK"
     gene            2062..2262
                     /locus_tag="PLM79_16300"
     CDS             2062..2262
                     /locus_tag="PLM79_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOV87919.1"
                     /translation="MSEGRVKWFNDKKGYGFIETKDQGDVFVHHSAIIAEGFRSLSEG
                     QRVSFEVEKGPKGPQAVKVRKL"
     gene            2595..4031
                     /locus_tag="PLM79_16305"
     CDS             2595..4031
                     /locus_tag="PLM79_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87920.1"
                     /translation="MNVVGDLLEKTCKEMIRTILCCIDSATKGTIYRIGPMPQLQAIR
                     ITSGVREDGTDQIRWGLPSVSDYNYPGKSWEQYRDRPNHVLEAMGWCVERQKSWTADN
                     PFEDVRSVGKQLRGEIEDFYHMEPVLVPKKDLYGACHGRVELPEDWQGNPIWHDSPYV
                     VAAVVKIHFKPYTLKRNDRSTKIIKELSRSLGTEILSLSLRETLFRAQKEFTRQRLHS
                     CESLAHELRNAFIKFGFIVSAINAEMGILRGYWEDHLRRVFPDLEWKEKILGRLNVLI
                     SQHRSGLGENPEWKKLAEALVKEQTELVALQLLPAQAEQWVRHKIRPKWKRLLVEYKG
                     LGKDEEEIVRLLGRLRDSLWLGMNPEILRQAEQIPRDLRDRWPPLVYSYYTAEKLPPL
                     REVLQWLEHPDLPVPHKLQIRKVLNCLKAMVEVVPEMEERTNRILLSLKSGGLQGPPI
                     PEALDHYCGSMGAGGSYLDPDELISMSI"
     gene            4054..5181
                     /locus_tag="PLM79_16310"
     CDS             4054..5181
                     /locus_tag="PLM79_16310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOV87921.1"
                     /translation="MPISRGPIRLLRTLPHTEFILSPSGMFLEIDPEGPAIFGYGSCR
                     EFFESQPFHRLCCAAEEGAALLRKLGEEGVVQDYVVEMKRKDGSTFAACITAQAVMEE
                     GGGVFYRGCLQDVTQAIRWHKALLECERLNEHLRESEDRTRRLNQYILQMLMIMSHDI
                     RGPLVAIAATLKLLIRGVYGTMDESVERTLQDLLSRVVRLHGIAEDCLGRAHSVEGSM
                     EVSKEIFDLRQDIIDPVLDELANEIQQREITIDNRLGAIPAGAIQVSVNKVWLKVVYR
                     NLFSNAIKYGGRGCTIAFGFEDHGDLYRLNVYNSGEVVPLEHRDKLFNKFYRVETGDK
                     STSDGVGLGLYMTRQIIREHGGDIWYEARNDGSDFVFTLAK"
     gene            5190..6416
                     /gene="ilvA"
                     /locus_tag="PLM79_16315"
     CDS             5190..6416
                     /gene="ilvA"
                     /locus_tag="PLM79_16315"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOV87922.1"
                     /translation="MITIRDVRQAAEVLRGKILRTPLVYSSTFSKMTGCEVFLKLENL
                     QQTGSFKIRGATFKVLRRRSRIGGQGVVAASAGNHAQGVAMAAAQAGLPATILMPRWA
                     SITKQEATRSYGGQVILEGESIEDGIRKAKELAEGGKTFIHPFDDEDIIAGQGTLGLE
                     IVEELPDADTIVVPVGGGGLISGVSLAAKAHRPKIRVIGAQAAACPAAYRSFREGTPV
                     RVQAEKSIADGISIKQVGQIPFAVMRELVDDVLQVSEEEIGDSIVTLLERKKVLAEGA
                     GAVPLAVLLNSSLGKSCKGKVVLVISGGNVDHPLVERIIRRGLFRRGRIMRFSVCLED
                     APGSLHGLLECIAREEANVLHIYHDRGGRNLPVYQSRVELELETRGPGHAVKILDQLR
                     AAGYSVSPSRHDDAFE"
     gene            6504..>6727
                     /locus_tag="PLM79_16320"
     CDS             6504..>6727
                     /locus_tag="PLM79_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOV87923.1"
                     /translation="MKECDIKLYALSTCIHCRNTKEYLDGCGVKYDCVDVDKLEGDER
                     KEILEEIRKVNPHCSFPTLIIGDKVIVGFR"
BASE COUNT         1446 a         1924 c         2071 g         1286 t
ORIGIN      
        1 cttcccggct tcgctcagca ccgtatccag ttccctctcc gtgcggttca gcatctcttc
       61 cagctgcaag agcagcctgg gggggagctt ttcccccatg gccctgacgg cttcggcttt
      121 ccgcagggtt ctcatccagc tttgctccca cctgggtacc tgcctttgcc gggcggcggt
      181 ttcccggtcg gtggggggaa tcgggatcac ttcccccatg cccggcacat gggtggaaaa
      241 tccgtagcgc tgtcgcacga gggatgcaaa cacctcgcta atgctttctt ctccgtgaac
      301 ggcatagacc cgcaaggacg ggttcccgaa atgggcgatc cagtcgagca ggtcctgctg
      361 atcggcgtgg gccgaaaacc ccccgatggt gaataccctg gcccgcacca ggatcctctc
      421 tcccaggacc ttgacggagg gggctccttc caccagcttt ctcccgacag tgccctcggc
      481 ctgaaacccg acgatgacca ctgcgcagcc cttgcgccac aggttgtgtt tgaggtggtg
      541 cttgatccgg cccgcggtgc acatgccgtt gccggcaatc acgatggcgg ggccggcttt
      601 ctggttgatg gcgatcgaat cttcggcggt ccgggagaaa atcagctgag ggaaatcgaa
      661 agggtcctgt ccgtggttga ggatggccat ggcgtcctcg tcgaagaatt cgctcctgcg
      721 tcggaagatc tcggtggcgg cgatggccag ggggctgtcc aggtagaccg gcatggacgg
      781 gatgagaccc tttcgaaacg attctcccag gatgtagagg atctcctggg tgcgctccac
      841 ggcgaaggcc gggatgatca ctttctcccc gtggttgtag ctgtagtgaa tcgcctcgac
      901 gagttccctg cggctctgtt ccatggtctt gtggtttcgg ttgccgtagg tggattcgac
      961 gaacaggata tccgcgtcgc cgatggcttc cgggtctcgg acaatgagct ggtctttctg
     1021 gccgatgtcc ccggaaaaca ccactttgtg gggcttgtcc cgggggccga cccacagctc
     1081 gaggatcgag gagcccagga tatggcccgc attgcggaag gagaaagata ggtgcggatg
     1141 aaggtcgatt ctctctccca cgggcacggt ctccaagaac gggaacgaag cctctgcgtc
     1201 cggcaacgta tacaggggtg tcatctcggg gtctccccgc ctccttcgtt tgcggctttg
     1261 ccattcggct tccatctcct ggatgtgcgc cgaatccatg agcaggatct tgcatagctc
     1321 cgccgtgggc agggtacaga agatccttcc ccggaagccg tctctgacta gctttgggat
     1381 ccgtccgcag tggtcgatgt gggcatgagt gagaaagagg gctgagatct cttccggacg
     1441 aaatccccat tccttccggt tcaacgcttc catctgcttt ccgccctgaa aaaggccgca
     1501 gtcaatgagg tatcgttctt cgtcgtccag gagaaaacag gacccggtca cgcagcgtgc
     1561 ggcgcccaaa caggtgattt gcatggtcca cctttctgtc ttgaaggctg gagagacggt
     1621 tgtaggccgg catcggggtc cgaggactcg atgggggcgc ccccgggcaa cttcggggaa
     1681 tcccgcatcc gtcgaatccc ttttcggcat ggggcgggtc gccgcagggg gcggcggacg
     1741 gttcccgcgc cccataactt aaatgggaag gcgccggttt tgtcaacgga agtttgggaa
     1801 catccttcgg gggaagaagg tgaaaaagag cggcaacgga gggagcggat ggcgggggcc
     1861 aagagcaggt aaggcattga gataacgaaa gccttgacct ttgagaaata ttgcgctaag
     1921 tagcacctca tctttgtaaa gaggtcgtat tttgaggtcc gccccaatct gttttcgatc
     1981 ccaaaaaacg atggcaaggt cgctctcaaa ggtacgcacc gacattccga aacaaaacca
     2041 tggcaggaaa ggaacaccaa gatgtcggaa ggccgagtaa agtggttcaa tgacaagaaa
     2101 ggctacggat tcatcgaaac caaggaccag ggggacgtct tcgtccatca ctctgcgatc
     2161 atcgccgaag ggttccgctc cttgagcgaa ggacagcggg tcagtttcga agtggaaaag
     2221 gggcccaaag gacctcaggc cgtaaaagtt cgtaaactgt agagagccac aatcgcttcg
     2281 actgcggaat agtagcgaac agccgggaag gttgcccggc tgttcccatg agaatcatcc
     2341 ctctcccatc cccgatatcc tttccctgca gccctgcacc ctcagcatct cgccggaaac
     2401 ggcccgtgtt cacaaatgag ctggaaacct ccccctgaaa ctggtagaat ccaggagcct
     2461 atcggacggg ctggaatccg ttcttgaaga acgagagcgg gtgtttcccc gatggaagtc
     2521 cgggtcctct gcaaggcctt gaaggtggga aagagaaagc acgaagcttg agagggggat
     2581 gttggagcgg gaaaatgaac gtcgtcggtg acctcctcga gaagacctgc aaggaaatga
     2641 ttcggaccat cctctgttgc attgacagcg caaccaaggg aaccatctac agaatcggtc
     2701 ccatgcctca gttgcaggcg atccgaatca cgtccggggt tcgagaagac gggacggatc
     2761 aaatccgctg gggtcttccc tccgtttcgg actacaacta cccgggaaaa agctgggaac
     2821 aatatcggga ccggccaaac cacgtgctcg aggccatggg ttggtgcgtg gaacgccaga
     2881 aaagctggac ggccgacaac cccttcgagg acgtccggag tgtgggaaag cagctgcgcg
     2941 gggagatcga agatttctat cacatggagc ccgtcctggt tccgaagaag gacctgtacg
     3001 gagcctgcca cggtagggtc gaactcccgg aagattggca gggaaatccc atctggcacg
     3061 attccccgta cgtggtggcc gccgtcgtca agattcattt caaaccctac actctaaaga
     3121 gaaacgaccg atctacaaaa atcatcaagg aactttcccg cagcctgggc acggagatcc
     3181 tttctttgag cctcagggaa accctgttca gggctcagaa ggagtttacc cgccagcgcc
     3241 ttcacagttg cgaaagcctt gcccacgagt tgcgaaacgc gttcatcaag ttcggtttca
     3301 tcgtctcggc catcaatgcc gagatgggga ttcttcgggg atattgggag gaccatctgc
     3361 gccgggtctt tcccgatctc gagtggaagg agaagatcct ggggcgactg aacgtgctga
     3421 tttcccagca ccggtccggt ctcggggaaa acccggaatg gaagaaactg gcggaggccc
     3481 tggtgaagga acaaacggag ctcgtggcgc tccagctgct tcccgcccag gcggaacaat
     3541 gggttcgtca caagatccgg cccaaatgga aacgccttct cgtggaatac aagggtttgg
     3601 gaaaggatga ggaagaaata gtacggctgc tggggcgcct gcgggattct ttgtggctgg
     3661 ggatgaaccc ggagatccta cgacaggcgg agcagattcc ccgagacctt cgggaccgtt
     3721 ggccccccct ggtatacagc tattacaccg cggaaaagct tccgccccta agggaggtcc
     3781 ttcagtggct ggaacatccg gacctgcccg tgccccacaa gctgcaaatc cgaaaggtgc
     3841 tcaattgcct caaggccatg gtggaggtgg ttcccgaaat ggaagagcgg accaaccgaa
     3901 tcctcctgtc gttgaaaagc ggcggcctgc agggaccacc catccccgag gcgctggacc
     3961 attattgcgg ctccatggga gcagggggct cctacctgga tcccgatgaa ctgatatcca
     4021 tgtcgatctg agcccggagc aaaggacatt cccttgccga tttcaagagg acccatcagg
     4081 ctcttacgaa cgcttcctca cacggaattc atcttgagcc cttcggggat gtttctggaa
     4141 atcgacccgg agggtcccgc catttttggg tatgggtcct gtcgggaatt cttcgaatcc
     4201 cagcctttcc accgcctttg ctgtgccgcg gaggaggggg ccgcccttct tcggaagctc
     4261 ggggaggaag gggttgtcca ggactatgtg gtggagatga aacggaagga cggcagcacg
     4321 tttgccgcgt gcatcacggc acaggccgtg atggaagagg ggggaggcgt cttctaccgg
     4381 ggctgtctgc aggacgtgac ccaggccatc cggtggcata aagctctcct cgaatgcgaa
     4441 agactcaacg agcacctgcg ggagtccgag gatcgcacgc gacggctcaa tcaatacatc
     4501 ctccagatgc tcatgatcat gtcccacgat atccgggggc ccctggtagc cattgcggcc
     4561 actttgaaac tgctcatccg gggggtctac ggcaccatgg acgaaagcgt cgagaggacc
     4621 ctccaggacc tgctgtcccg ggtcgtccgg ttgcacggaa ttgccgaaga ttgcctgggc
     4681 agggcccatt cggtggaggg gtccatggag gtgtccaagg agatcttcga ccttcgtcag
     4741 gacatcatcg atccggtgct cgacgaactg gccaacgaaa tccagcaacg ggagatcacc
     4801 atcgacaacc gcctgggggc catccccgcg ggggccatcc aagtcagcgt aaacaaggta
     4861 tggctcaagg tggtgtaccg aaacctgttc agcaatgcga tcaagtacgg cggaagaggc
     4921 tgcaccatcg cattcgggtt cgaggaccac ggcgacctgt accgcctcaa cgtgtataac
     4981 agcggggaag tggttcccct cgaacaccgg gataagttgt tcaacaagtt ctaccgggtc
     5041 gaaaccgggg acaagagcac atcggacgga gtgggcctgg gcctctacat gacccgccag
     5101 atcatccgcg agcacggggg agacatctgg tacgaagccc gcaacgacgg ttccgacttt
     5161 gtcttcactc tcgcgaaatg acctcgccca tgataaccat ccgagacgtt cgccaggctg
     5221 cggaggttct ccgggggaag attcttcgca cccccctggt gtattcgtcc acgttcagca
     5281 agatgaccgg ctgtgaggta ttcctgaagc tcgaaaacct ccagcagacc ggctccttca
     5341 agattcgcgg cgcaaccttc aaggtccttc gccgaaggtc ccggatcggg ggacaggggg
     5401 tcgtggccgc ctcggccggc aaccacgccc agggtgtggc catggccgcc gcccaggccg
     5461 ggcttcccgc caccatcctc atgccccggt gggcttccat caccaagcag gaagccactc
     5521 gaagctacgg agggcaggtg atcctagagg gagaaagcat cgaagacgga atccgcaagg
     5581 ccaaagaact ggccgaaggg ggaaagacct tcatccatcc cttcgacgac gaggacatca
     5641 tcgcgggcca ggggaccctg ggtctcgaaa tcgtcgaaga actccccgac gcggacacca
     5701 tcgtggtccc cgtgggggga ggggggctca tcagcggggt gagccttgcc gccaaggccc
     5761 atcgccccaa aatccgagtg atcggagctc aggcggccgc ctgccccgca gcttaccggt
     5821 cctttcggga agggaccccg gtccgggtgc aggctgaaaa gtccatcgcc gacggaattt
     5881 ccatcaagca ggtagggcaa atcccctttg ccgtcatgcg ggagctggtg gacgatgttc
     5941 ttcaggtgag cgaggaagag atcggagact ccatcgtgac cctcctggag aggaaaaaag
     6001 ttttggccga gggggcgggg gccgttcccc tggccgtact gttgaactcc tccctgggga
     6061 agtcgtgcaa gggcaaggtg gtgctggtca tcagcggcgg caatgtggac catccgctgg
     6121 tggagcgaat cattcgccgg ggactcttcc gccggggtcg gattatgagg ttttcggttt
     6181 gcctggagga cgcccccggg tccctccacg gactcctcga atgcattgcc cgcgaggagg
     6241 ccaacgttct gcacatttac cacgaccggg gggggaggaa cctgccggtg taccaaagcc
     6301 gggtcgagct cgagctggag acgagaggcc ccgggcacgc cgtgaaaatt ctggaccagt
     6361 tgagggctgc cggctactcg gtgagcccct cgaggcacga tgacgccttc gagtagccgt
     6421 cggccggaac cctaccgccg accgctcgcg aacgtgtctc cacccgtcaa ccacccctaa
     6481 aggccttcca gcaggagatg agaatgaaag aatgcgacat caaattgtac gccctgagca
     6541 cctgtattca ttgccgaaac accaaagaat acctggacgg gtgcggggtg aaatacgact
     6601 gcgtggatgt ggacaaattg gaaggggacg agcgcaaaga gatcctcgag gaaatccgga
     6661 aggtgaatcc ccactgctct ttccccaccc tcatcatcgg ggacaaggtc atcgtcggtt
     6721 ttcgaaa
//
LOCUS       DAOOVC010000190         6700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6713_length_6700_cov_5.513770, whole genome shotgun sequence.
ACCESSION   DAOOVC010000190 DAOOVC010000000
VERSION     DAOOVC010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6713_length_6700_cov_5.513770"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLM79_16325"
     CDS             <1..279
                     /locus_tag="PLM79_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV87924.1"
                     /translation="TCSFLEQGGSYDVMIVDYMMPELTAGELLERTRGYLSRDGFIIL
                     ISGHTDVVEQLNLKSMGVDIFLPKPLDLELLWEAVGSGGKAESRSMPT"
     gene            295..1677
                     /locus_tag="PLM79_16330"
     CDS             295..1677
                     /locus_tag="PLM79_16330"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOV87925.1"
                     /translation="MKERIGFWQNLYQGLGSLKLALFNLLILAAASVLGTLLPQGASQ
                     EDLQAHYSRAAAGLIELLGLQDLYHTNWFRFLLVLLGVSLIVCTIQRLPKTLRLLRHR
                     EESPTRAKLLKFSHSKEITTVLPIDEAQAHLTRIIAREFAPVRSLESTAGYAGIAEKG
                     RWSRLMVYVVHLSVLMILLGALLGSVFGFKGTMNIPEGASSDEVALSSGHQSMTLPFK
                     VRCDDFDVSYYDTGAPKEFRSDLVLLEEGKEPVQRAIRVNEPLSYRGISFYQASYGST
                     VKQAEVDLTNQDSGKTTRLTLPLGEPMTIPDANDVIELVNYQQNLSGLGAAVGISLKK
                     GEETQPFGSWILVDQPEFHGNRIKNYRIKPTGVDTVQYTGIQVKRDPGIWMIYVGFIT
                     LVVSMGLSFYTSHRKLWVLAYPDRNRTRIIIAGRNNKSSVELERKLDGLYARLHEALN
                     SDEHTKKARA"
     gene            1677..2522
                     /gene="ccsB"
                     /locus_tag="PLM79_16335"
     CDS             1677..2522
                     /gene="ccsB"
                     /locus_tag="PLM79_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOV87926.1"
                     /translation="MNSSLLLSLTTFGYLFCTLLYFSGVVFRFAPLLRWGGIVGFVVL
                     AVQSTGIVLRWVESYAMGYGHAPLSNMYESLVFAAWAIMLIYLIMQWRTGQGSLGVFP
                     SLFAFLAMAYASFSTSVDSKIQPLLPALKSNWLIAHVITCFFGYAAFAVSVGISLVYL
                     AKKRRALDSGSEGILGFFPEPKALDELNYRMILFGFLWLSLGIITGSVWANSAWGTYW
                     SWDPKETWSLITWLIYAAMLHARSMQGWRGNRVAWLSLVGFACVLFTYFGVNFLLSGL
                     HSYGS"
     gene            complement(2932..4800)
                     /locus_tag="PLM79_16340"
     CDS             complement(2932..4800)
                     /locus_tag="PLM79_16340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOV87927.1"
                     /translation="METQQPMNGDPNKSLEVGVVLENKWVILDFIGKGGMGEVYRAHQ
                     LNLDRDVAIKIISQKWLEEINDNDYEAETCVERFLREVKVMAQVRHPDVIQIFDYGTT
                     SIGKGGQDLVIEYIAMEYIPGGTLRSTMSAEGYYPDEERTREWLTLYFLPILDGVCAL
                     HEKGIVHRDLKPENVLLDGKTPKIADFGLARSCRLGPVTESMDIKGTPPYMSPEHFMD
                     LKRTDERADVYSLGKILYEALSGKMKQDQIPFRKASLAEPETPFFQDLDRIIQEATNE
                     DKRERLPSVKSFRQTILDTLETEIKPSPPEAEKAPVLKHKRWKTLVAAPLLLLIGLGI
                     YLFHEESHVSAPSSHSVSPGITQEKTQEKGSEHGAPPPPGVKGQDQDIMRLIPGGTIT
                     LPPSFGPDAGRTIQVESFYLDETLVTNYQYVEFLNRVLSKIKVEDGVVWGDGKVWLML
                     GEVKGGYEPIVFEDGKFRLQLSAYSSHPVLRVSAQGASAYVQFHGSRLITEDEWLYAV
                     ITGKRAVSSESTLQRPVPSASDENAAPSPVLIYQPDAYGIRGMNGSVGEWGVRAWKTS
                     FEGDNKTQDYIVLGACAGSREIGIQGPSPSAVARRPWEASEEVGFRCARSTTPESG"
     gene            complement(4958..>6700)
                     /locus_tag="PLM79_16345"
     CDS             complement(4958..>6700)
                     /locus_tag="PLM79_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV87928.1"
                     /translation="HGFWKFVLSSVEHGLYATDFDFLFKGIGILFAIYLASAFLLYMQ
                     NSLLAHLSQRVVLRVRENLFSHLLFLPHNFFEKVRTGDLTSRISKDTADIQDILEGAI
                     TIMVRSLPTILGILIVSFALDWVYALTFIFVIPLIFWANVLFARSAKEAVRRQRRIEG
                     AMASRAQEAFCHHKAVATLSLENELVEDFRENGRESATHGVDAGRFQGMLTASMDFLV
                     GVTTLAVLLIGGLRTLHGCLTVGQLMVFLSYLNSIFKPIRETSKFTIRLAKSATAVER
                     VEEIMHMSPHEIGATELPHAMKHRRFKGHIEFRDIVFGYQPNSAVLQAIDFSVAPGKM
                     VALVGDSGSGKSSLLHLLMRLYDPQQGVIRIDGTEIRAMKLSSLRKQLSVVLQDSYIF
                     NMTILENIGLAKPGATRAEAIKAAKAAGAHGFIQRLPQGYETPLGEGGSGLSGGQKRR
                     LAIARAILRDAPIVLLDEPTAGLDAASERKVVEALKRLTRNKTTFIVTHQLSTVVDAD
                     LILVLSGGRIVERGVHEQLLRQGGFYASLWQAQHHNGSARDHRPFREPIEITELDKEE
                     ESERSVASRLSMRL"
BASE COUNT         1436 a         1828 c         1820 g         1616 t
ORIGIN      
        1 acctgctcct tcctggagca gggagggtcc tacgatgtca tgatcgtgga ttatatgatg
       61 cccgaactga cggctggtga actgctggag cgtacgagag ggtatctctc gcgagatggt
      121 tttatcattc tcatcagtgg gcacaccgat gtcgttgaac agttgaacct gaaatccatg
      181 ggagtagaca tctttctgcc caagcccctg gacctggagc ttctctggga agcggtgggg
      241 tccgggggga aggcggaaag tcggtccatg ccaacatagc acgaggtagt gtgaatgaag
      301 gaaagaatcg gcttttggca aaacttgtat caaggcttgg gctcactgaa gctggcgttg
      361 ttcaacctgc tgatccttgc ggcggcatcc gttctcggga cgctgctgcc ccagggcgct
      421 tcgcaagagg atttgcaggc tcattacagc cgggcggccg ccgggttgat cgagctgttg
      481 ggtttacagg acctctacca cacgaattgg tttcgtttcc tcctggtgct cctgggtgtg
      541 agcctgatcg tttgtacaat acagcggctg cccaaaacgc tcagacttct tcggcaccgg
      601 gaagaatcgc ctactcgcgc aaagctgctc aagttctccc acagcaaaga gatcaccacg
      661 gttttgccca ttgatgaggc acaagcccat ctgacgcgga tcatcgcccg ggaattcgca
      721 ccggtgcgct ccctggagag tacggcaggg tatgccggta tagccgaaaa agggcgatgg
      781 agccgtctca tggtctacgt ggtccacctg agcgtactca tgatcctgct cggtgcgttg
      841 ctgggatccg ttttcgggtt caaggggact atgaatattc ccgaaggagc ttcatcggac
      901 gaggtggctc tctccagcgg acaccagtcg atgacgctcc ctttcaaggt gcgctgcgat
      961 gacttcgatg tcagctacta tgataccgga gcccccaagg aatttcgatc cgatctggtc
     1021 cttttggaag agggcaagga gcccgtccaa cgggccattc gcgtaaatga gccactcagc
     1081 taccggggaa tcagctttta ccaagcctcc tacggctcca ctgtcaaaca ggcggaagtg
     1141 gatctcacca atcaggattc gggtaagacc accaggctga cgctgccgtt gggagagcca
     1201 atgaccatcc cggacgccaa cgatgtgatt gagctagtca attatcaaca aaacctcagt
     1261 ggactcggcg ctgcggtcgg catcagtcta aagaagggcg aagagacaca gcctttcgga
     1321 tcgtggattc tcgtggatca gccggaattt cacggcaaca ggatcaagaa ttaccgtatc
     1381 aagccaacag gtgtcgatac tgttcagtac acaggcatcc aggtcaagcg cgatccgggg
     1441 atctggatga tctacgtcgg cttcatcact ttggtggtga gcatgggact cagcttctac
     1501 accagtcacc gcaaattgtg ggtcctggcc taccctgatc gcaatcgcac ccgcatcatt
     1561 atcgctgggc gcaacaacaa aagctccgtc gagctggaac ggaagctcga cggattatac
     1621 gcgagattgc acgaagcact gaattccgat gagcacacca agaaagcgag ggcctgatga
     1681 acagctcact tcttttgagt ctaaccactt tcggctacct gttttgcacg cttctttact
     1741 tttccggggt tgtctttcga ttcgcccccc ttctccggtg gggcggcatt gtgggattcg
     1801 tcgtccttgc ggtccaaagc acgggtatcg ttttgcggtg ggtggaatcc tatgcaatgg
     1861 gctacgggca cgcgcctctc tccaatatgt atgaatcgct cgtattcgcg gcgtgggcga
     1921 tcatgctgat ctacctcatc atgcaatggc gcacgggaca ggggtccctt ggggttttcc
     1981 cgtccctgtt cgctttcctg gccatggcct atgcctcctt ttccacctcc gtcgattcta
     2041 aaatccagcc actcctgccg gcactcaaaa gcaactggct tatcgcacac gtgatcacct
     2101 gcttttttgg atatgccgcc ttcgcggtct ccgtcggcat cagcttggta tatctcgcaa
     2161 agaagcgaag agcgctcgat tcgggctcgg agggtatcct gggcttcttt ccagagccga
     2221 aagccttgga tgagctcaat tatcgcatga ttctattcgg gttcctttgg ctttctcttg
     2281 gaatcatcac cggttcggtg tgggccaact cggcctgggg cacatactgg agctgggacc
     2341 cgaaggagac atggtcgctc atcacctggc tgatctacgc ggccatgctg cacgcacgct
     2401 ccatgcaggg ctggcgcgga aaccgcgttg cgtggctctc gctggtcgga tttgcttgcg
     2461 tgctcttcac gtacttcgga gtcaacttcc tgcttagcgg tctgcacagc tacggcagtt
     2521 agaggggttt gaaaaggatt gcctcaacgt aaggaggggg cgggccttcg ttgaggcaat
     2581 cccgagagca aggaggattc ccaagctccg gggctggacg tgaagcaggc taagcggctg
     2641 gtgcgtcatg gtgttattag cctcggaaaa gtgatgcgcg gctgcccgaa ccgctagttc
     2701 ctgcttcgac cgtccagctc cgggtgtttt atcctccctg ccgggtctac gtgtggcgtg
     2761 ttgccaggat cacctcctaa cgatgccctc cgccgtgaca ttcaccattc taaccagtcc
     2821 tcgatgggta gcagcaaaaa tcttcatatc gtccttgcgt gtttgagctc actgagaatc
     2881 agctctctta ccggcttcca cactttgctc ggtcaggccg agcaaagagg atcaaccgct
     2941 ttcgggtgtg gtactcctgg cgcatcgaaa tcccacttcc tcggaggcct cccacggtcg
     3001 ccttgcaacg gcggaagggg atgggccttg tatgccaatt tccctgctgc ctgcgcacgc
     3061 tcccagaact atatagtcct gtgttttatt gtctccctcg aaggacgttt tccatgctcg
     3121 taccccccac tcccccacgc ttccattcat gcctcggatg ccatatgcgt ctggttgata
     3181 gatcagtacc ggggaaggtg ccgcattctc atcagaggca gacgggactg ggcgctggag
     3241 ggtgctctcc gaagagactg cccgttttcc ggttattaca gcgtagagcc actcgtcttc
     3301 cgtgatcagg cgacttccat ggaactgcac gtaagcagac gcgccctgag cgctcacacg
     3361 aagcaccgga tgagaggagt aggccgaaag ctgaaggcgg aactttccgt cttcgaaaac
     3421 aatgggctca tatccccctt tcacctcccc gagcatcagc catacctttc catcgcccca
     3481 aacaacaccg tcttccacct tgatttttga aagcaccctg ttcaggaatt ccacatactg
     3541 gtagttcgtg accagcgtct cgtccagata gaaggattcg acctgaatgg tccttccggc
     3601 atcagggcca aaagatggag gaagtgtgat cgtacctccc gggataagac gcatgatgtc
     3661 ctggtcctga cctttcacac cggggggggg aggagcacca tgctctgatc ctttctcctg
     3721 cgttttctcc tgtgtgatac cgggcgaaac ggaatgagag gatggtgccg acacatgaga
     3781 ctcttcatgg aatagataga tcccgagacc aatgaggagc agcagaggtg ccgcaacgag
     3841 cgtcttccat cgcttgtgct tgagcaccgg agctttttct gcctctggtg gcgatggttt
     3901 gatctcggtt tcgagggtgt ccaaaattgt ttgcctaaag gattttaccg atggaagccg
     3961 ctctcttttg tcttcattcg ttgcttcctg aatgatgcgg tcaagatcct gaaagaaggg
     4021 ggtctcaggt tcggcaagac tcgcttttct aaaagggatc tggtcctgct tcatcttccc
     4081 cgaaagagcc tcgtagagga tctttcccag agaatagaca tcggcccgct cgtccgttct
     4141 cttgaggtcc atgaaatgct ccggagacat gtagggcggg gttcccttaa tatccatgga
     4201 ctccgttacg ggaccaagcc gacaagaacg ggcaagacca aaatccgcaa ttttgggagt
     4261 ttttccatcg agtagaacgt tttccggttt gaggtcgcgg tgcacgatcc ccttttcgtg
     4321 aagagcgcaa accccatcca atatgggcag gaaatacaga gtgagccatt cccttgtgcg
     4381 ctcttcatcg gggtaatacc cttccgccga catcgtggag cgcaatgtgc ctccagggat
     4441 atactccatt gcgatgtatt cgatcaccag atcctgtcca cccttaccaa tggatgtggt
     4501 accatagtca aagatctgga tcacatctgg atgtcgaacc tgggccatga ctttgacttc
     4561 cctgaggaac cgctcaacgc aggtttcggc ctcataatcg ttgtcgttga tctcttcaag
     4621 ccatttctgg gagatgatct tgatggcgac gtcacggtcg aggttgagtt gatgtgcccg
     4681 gtaaacctca cccattccgc ccttcccgat aaaatcgaga atcacccatt tgttctcaag
     4741 gaccacacca acctcaaggg atttattagg gtcgccattc atcggctgct gtgtttccat
     4801 atgttcacta ctcttcacgt gtcaccattt gcggcatttg taggtacagt acacttcctc
     4861 ttcggttcct gctcgacctc tcgccctcca gcctctgaac ttgagcggtt atctccgtgt
     4921 gatcaggaga acgctctctt gacaatagat atgtaaatta cagtctcata gagagccggc
     4981 tggcaacgga ccgttcagat tcttcctctt tatccagttc cgtgatctcg atcggctccc
     5041 gaaaggggcg atggtctcgg gcggacccat tgtgatgttg ggcctgccag agacttgcat
     5101 aaaagccccc ttgccggagg agttgctcgt gaacccctcg ctctacaatt cttccgccag
     5161 aaaggaccag tatcaggtcc gcatctacta cggtagaaag ctgatgagtg acgataaagg
     5221 tcgtcttgtt acgggtaagt ctcttcaacg cctcaacgac ctttcgctct gatgctgcat
     5281 ccagccccgc agtaggttca tccagaagga cgatgggggc atctctcaaa attgcgcggg
     5341 caatagccaa ccgtcttttc tgacctcccg aaagtcctga cccgccttcc cccagaggtg
     5401 tttcgtatcc ttgggggagt ctctggatga acccatgggc tccggcagct tttgcagctt
     5461 tgatggcctc ggccctcgtc gcaccgggtt ttgcgaggcc aatattttcc aagatggtca
     5521 tgttgaagat ataggagtcc tgaagcacaa ccgagagttg tttgcgaagc gaggagagct
     5581 tcatcgcacg aatttccgtg ccgtcgattc gaatcactcc ctgctggggg tcgtagagtc
     5641 gcatgagaag gtgaagtagg ctggacttac cgcttcccga gtctccgaca agggcaacca
     5701 ttttgcccgg tgcaacagag aagtctattg cctgcagcac ggcactgttt ggctggtacc
     5761 caaagactat gtctcgaaac tctatgtgtc ccttgaaacg gcggtgcttc atggcatgcg
     5821 gcaattcggt tgctcctatc tcgtgagggc tcatgtgcat gatctcctca acgcgctcga
     5881 cggctgtcgc ggatttggcc aggcggattg taaatttgct ggtttctcga atgggcttga
     5941 agatgctgtt gagataggaa agaaagacca tgagctggcc tacggtaagg cagccgtgca
     6001 gggtgcgaag cccgcctatg agcaggacag ccaaggtggt aacgccaacg agaaaatcca
     6061 tggaggccgt cagcattccc tggaaacggc cggcatcgac accgtgagta gcactctccc
     6121 tgccgttttc tctaaaatct tctaccagtt cgttttccag agagagtgtt gccacggcct
     6181 tgtgatggca gaatgcctcc tgcgcgcgcg aagccatggc tccttcgata cgtctttgtc
     6241 gtctcactgc ttccttggca cttcgggcaa agagcacgtt tgcccagaag atcaagggaa
     6301 tcacgaaaat aaaggtgagt gcgtaaaccc agtcaagagc aaagcttacg atgagtatac
     6361 caagtatggt cggcaggctg cgcaccatga tggtgatcgc cccctccagt atgtcctgga
     6421 tatctgccgt gtccttggat atgcggctgg tgaggtctcc cgttcggacc ttctcgaaga
     6481 agttgtgggg aagaaagagg aggtgtgaaa agagattctc ccgcactctc agcaccaccc
     6541 gctgactcag gtgtgcgagc agactgtttt gcatgtagag caagaaagcg ctggcgaggt
     6601 atatggcaaa gagaatgcct attcccttaa acagaaaatc gaaatctgta gcgtacagac
     6661 catgctcgac ggatgaaagg acgaatttcc aaaagccgtg
//
LOCUS       DAOOVC010000191         6491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_6945_length_6491_cov_6.329708, whole genome shotgun sequence.
ACCESSION   DAOOVC010000191 DAOOVC010000000
VERSION     DAOOVC010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6945_length_6491_cov_6.329708"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLM79_16350"
     CDS             <1..285
                     /locus_tag="PLM79_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOV87929.1"
                     /translation="EADRVTGKQDAQLLVSDPFSWSEEVAEPRNWLGGKTRGKHSGCG
                     WENLAEILEGGREGLCPPWKISARGSLWWKIRTHQNHFELIRSWFLKAGR"
     gene            324..1154
                     /gene="cysQ"
                     /locus_tag="PLM79_16355"
     CDS             324..1154
                     /gene="cysQ"
                     /locus_tag="PLM79_16355"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOV87930.1"
                     /translation="MGVLEIDLEAVRAVIFEAGKAILEVYRAEDFHVRSKEDRSPLTL
                     ADTRSHEILGRGLRSLYPGIPLLSEEGRETSFAVRKEWPVFWLVDPLDGTKEFIKKND
                     EFTINVALVEAHRPVLGFVYLPVLDLFYIGDVREGCWEVRREERKPLKAAVPDGGKPV
                     RVLKSRSHPSPQVEDLLSLLPSHESMDRGSALKFCAVASGEAEFYPRFGPTWEWDTAA
                     GQAVVEAAGGIVVDPWGNPLSYNKPDLLNGPFLAAADLEWLRNSGLLEKASDLVHKKH
                     "
     gene            complement(1151..2281)
                     /locus_tag="PLM79_16360"
     CDS             complement(1151..2281)
                     /locus_tag="PLM79_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV87931.1"
                     /translation="MNLPDDCGSAPWLWEEILACMQQCVLVMDAEGLIHYSSSTVQNT
                     LGFDPRELKGTNLARLLTPEDLQFLYPNLLCLAANRRAFEGEVMLVRKDRTRFIAFLV
                     TRPCRDLMRGLDWVIVSVEDIHEQKELEKKIIKSHYQDLIQMANGIAHEIRNPLVGIG
                     GFVNRLYHAYGAGQEYREYYDLIMENLRRIENVIQKVEFFARLPEPSFKETSLGNALN
                     RAAESFADQLKARGIRFVSSLGEVTLRIDENLLERVFSVLLENSLAALPDGGTITVDA
                     QMDRAAFTVRFSDNGSGISPEHLPHVFNPFFSTKADGVGIDLALVKRILEIHRGSVGV
                     QSVLGEGTTFLLEFPLDRRRPIRTCLFPREQGAGGKPRDLFA"
     gene            complement(2639..4096)
                     /locus_tag="PLM79_16365"
     CDS             complement(2639..4096)
                     /locus_tag="PLM79_16365"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HOV87932.1"
                     /translation="MTEKKKLTTNAGAPVADNQNIMTAGPRGPALLQDVWFLEKLAHF
                     DREVIPERRMHAKGSGAYGHFTVTHDITQYTKAKIFSQVGKKTDLFVRFSTVAGERGA
                     ADAERDIRGFAIKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPRTNLRSAR
                     NNWDFWTSLPEALHQVTIVMSDRGIPATYRHMHGFGSHTFSLINAKKERFWVKFHLRT
                     QQGIRNLTDEEAEAVIGKCRESHQRDLYESIEKGDFPRWTFFIQVMPEKDAARCPYHP
                     FDLTKVWFHKDYPLIEVGVLELNRNPENYFAEVEQSAFNPANVVPGIGFSPDKMLQGR
                     LFSYGDAQRYRLGVNHHLIPVNASRCPFHSYHRDGAMRVDGNYGSTLGYEPNSYGEWR
                     DQPDFSEPPLSLEGAADHWNHREDEDYYSQPGKLFRLMGPEKQALLFANTARAIHDAP
                     REIQVRHIGNCLKADPAYGKGVADALGIPLSEVPA"
     gene            complement(4285..4716)
                     /locus_tag="PLM79_16370"
     CDS             complement(4285..4716)
                     /locus_tag="PLM79_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOV87933.1"
                     /translation="MQDRMERLLDKLREQGHRITPQRIAVLRVLLESRDHPSVEDIFE
                     KVKVNFPTTSLATVYKTISVLKELGELLELEFSNDHNRYDGNKPYPHPHLICTRCKKI
                     VDPELGGLTDITRELVSDTGYKILSHRLEFYGICPECQERR"
     gene            complement(4822..5697)
                     /locus_tag="PLM79_16375"
     CDS             complement(4822..5697)
                     /locus_tag="PLM79_16375"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOV87934.1"
                     /translation="MPPSSLEAEAGERVPVPGRLQPVDAERVLMRTRVGEVARRIGQA
                     LGELFCPASCAGCGRTWLESRDGFWCGSCREGLPWIRSPKCPLCGRPFPKSPSSSDHL
                     CGNCLRGEFHFQCARSATYHSGVVRDRIHQFKFGGRLHWGPPLSELLVETARREGLTE
                     ADCIVPVPLHVRRLRQRGFNQAALLGRFLGARLHRPVRYDLLYRFQWTDPQTRLHREQ
                     RHANVKNAFRVGNPQHVKGLHVLLVDDVFTTGSTLNECARSLRRAGAGEVTALTVARA
                     LPDWKEDWKEKSGDA"
     gene            complement(5730..>6491)
                     /locus_tag="PLM79_16380"
     CDS             complement(5730..>6491)
                     /locus_tag="PLM79_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOV87935.1"
                     /translation="EWPARSREAEKIISFVQEQFGIRIAPESAIGLKPMSRRGTERLV
                     RRALRYAVEKELPSVTLVHKGNIMKFTEGAFQKWGYELAASEFPRETVSETRVREEYG
                     GVVPEGKIVVKDRIADSMFQQVLLRPEEYHVVATPNLNGDYLSDALAAQVGGLGMAPG
                     ANLGDRCGVFEATHGSAPKYAGMDKVNPSSLILSGCMMLEHLGWREAARLVEQALERT
                     ILERIVTYDLARLMEKAREVRCSEFARAVVERMQE"
BASE COUNT         1251 a         1960 c         1991 g         1289 t
ORIGIN      
        1 gaagcggatc gggtcacggg aaaacaagac gctcagctcc tggtctcgga cccgttctcc
       61 tggtccgagg aggttgccga gcccaggaac tggctggggg ggaaaacccg cggcaagcat
      121 tcggggtgcg gctgggagaa cctcgcggaa attctggaag gcggccgcga gggactgtgc
      181 cccccttgga agatttccgc ccggggcagc ctttggtgga agattcgcac ccaccagaat
      241 cactttgaac tgatccgcag ctggtttctg aaagccgggc gatgaaacgg cctgcggccc
      301 cgtcccagag gaggaacacc gatatgggag tccttgagat cgaccttgaa gcggtaagag
      361 ccgtcatttt cgaggcagga aaggcaattc ttgaagtcta cagggccgag gattttcacg
      421 tccggagcaa agaggaccgt tctcccctca ccctggcgga cacccgttcc cacgagatcc
      481 ttggccgcgg gctgcgatcc ctctatcccg ggattcccct gctctccgaa gaaggcaggg
      541 agacctcttt tgccgtccga aaggaatggc ccgtgttctg gctggtggac ccgttggacg
      601 ggacaaaaga gttcatcaag aaaaacgacg aattcaccat caacgtggcc ttggtggaag
      661 cgcacaggcc cgtcctgggg tttgtgtacc ttcccgtgct cgacctcttc tacatcggcg
      721 atgttcgaga gggatgctgg gaagttcgcc gagaggagcg gaaacccctg aaggcggccg
      781 ttcccgacgg gggcaaaccc gtccgggtcc tcaagagccg ctcccacccg tcgccccaag
      841 tggaagacct gctgagtctt cttccctccc atgaatccat ggatcgcgga tctgccctga
      901 aattttgtgc cgtagcttcc ggggaagcgg agttctaccc ccggttcgga ccgacgtggg
      961 aatgggacac ggcggcgggc caggccgttg tggaagcagc cggcggcatt gtggtcgacc
     1021 cctggggcaa ccccctgtcc tacaataaac cggacctgct caacggtcct ttcctggcgg
     1081 ccgccgacct cgagtggctt cgcaactcgg gacttctcga gaaggcctcg gacctggtcc
     1141 acaaaaaaca ctaggcaaat agatctcgag gtttgccgcc cgctccctgt tctcggggaa
     1201 agagacaggt ccgaatcggt cgccgacgat cgaggggaaa ctccagaagg aaggtggtcc
     1261 cctcccccaa gacggactgg actcccacac tcccccggtg gatctccagg atccgcttca
     1321 ccagcgccag gtcgattcca accccgtcgg ccttggtgct gaaaaaggga ttgaacacgt
     1381 gcgggagatg ttcgggagaa atccccgaac cgttgtcgga aaaccggacg gtgaaggccg
     1441 ctcggtccat ttgggcgtcc acggtgatcg tgccgccgtc gggaagggcc gccagggaat
     1501 tttccaggag aacggaaaag acccgctcca acaggttttc atcgattctc agggtgacct
     1561 cccccaggga ggaaacaaac cggattcccc gggccttcag ttggtccgca aaggattccg
     1621 ccgccctgtt cagggcgttc cccaaagagg tctccttgaa ggaaggctcg ggaaggcggg
     1681 caaaaaactc gaccttctgg atcacgttct cgattcgccg caggttctcc atgatcaggt
     1741 cgtagtattc gcgatattcc tgccctgccc cgtaggcgtg gtagagccgg ttcacgaacc
     1801 ccccgatccc caccagggga ttgcgaatct catgggcaat cccgttggcc atctggatga
     1861 ggtcctggta gtgggacttg atgattttct tctcgagttc cttctgctcg tggatgtcct
     1921 ccaccgagac gatcacccaa tcaaggccgc gcatcaaatc tcgacacgga cgggtaacca
     1981 ggaaggcaat aaaccgggtc cggtcctttc ggaccagcat gacctcgccc tcaaaggccc
     2041 ttcggttcgc cgccaggcaa agcaggttcg ggtacaggaa ctgaagatct tcgggggtca
     2101 ggagacgagc caaattcgtt cctttcagct cccgggggtc aaatcccaac gtgttttgga
     2161 cagtggaact cgagtaatgg atgagaccct cggcgtccat caccaggacg cactgctgca
     2221 tgcacgcaag gatctcttcc cacaaccacg gggcgctgcc acaatcatcc gggagattca
     2281 tccctcctcc tcgatcgggt cttctttaca tgccggtttt ggggatgggg cgtatcggcg
     2341 gtggtacgag cgtcgagtga ggatcatagg acagaggccg catcttgtct atgagtttcg
     2401 ggtgaagagg ggttgccccc tgccgggaag caacccacgg tctccctcct gcacccggac
     2461 gacgttccaa ccagaccgtg cgatccgctc cgggcattgg ccgccggtcc gttgccttcc
     2521 tccgttcgtc cagggcagga caaggactcg gcgggtgcag cccgattcga gacccccctt
     2581 cagaatcccg gcggatcccc gatccccaaa acgcgaaccg cccgaggcgg gtttcggcct
     2641 atgcgggaac ctcggacaag gggatgccca gagcgtccgc cacccccttg ccgtaggcgg
     2701 gatcggcctt gaggcagttg ccgatgtgac ggacctggat ctcccggggc gcatcatgga
     2761 tggcccgagc ggtgttggca aaaagaaggg cctgtttctc gggacccatg aggcgaaaga
     2821 gcttgccggg ctgggaatag taatcctcat cctcccggtg attccagtgg tcggcggccc
     2881 cttccaggct caggggcggc tcggaaaagt cgggttgatc ccgccactcc ccgtagctgt
     2941 tgggctcgta gcccagggtg cttccgtagt tgccgtcaac ccgcatggcc ccgtccctgt
     3001 gatagctgtg gaacgggcaa cgggatgcgt tgacgggaat gaggtggtgg ttcactccca
     3061 aacggtagcg ttgggcatcc ccgtacgaga agagccgtcc ctggagcatc ttgtcggggg
     3121 aaaagccgat gccgggcacc acgttggccg ggttgaacgc cgattgttcc acctcggcga
     3181 agtaattctc gggattgcgg ttcagctcaa gcacccccac ctcgatgagg ggataatcct
     3241 tgtgaaacca taccttggtg agatcgaagg ggtggtaggg gcaccgggcc gcatctttct
     3301 cgggcatgac ctggatgaag aaggtccatc gggggaaatc acccttttcg atgctctcgt
     3361 acaggtcccg ctggtgactt tcccggcact tgccgatgac cgcttcggct tcttcgtcgg
     3421 tcaggttgcg gatgccctgc tgcgtgcgaa ggtggaattt gacccagaac cgttccttct
     3481 tggcgttgat gaggctgaag gtatggctgc cgaaaccgtg catgtgtcga taggtggccg
     3541 ggatcccccg gtcgctcatg acgatggtga cctggtgcaa agcttcgggc aggctggtcc
     3601 agaagtccca gttgttccgg gcgctgcgta agttggtcct gggatcccgc ttgacggcgt
     3661 gattcaagtc cgggaacttg aggggatcgc gcaggaagaa gaccggcgtg ttgttgccca
     3721 ccaggtccca attgccctcc tcggtataga acttgatcgc aaagccccgg atatcccgtt
     3781 cggcgtcagc ggcgccgcgt tccccggcca ccgtggaaaa ccggacgaag aggtccgtct
     3841 tcttccccac ctgggagaat atcttggcct tggtgtattg ggtgatgtcg tgggtcacgg
     3901 taaaatgtcc gtaggctccg gatcccttgg cgtgcatacg gcgctcgggg atcacctccc
     3961 ggtcgaaatg ggcgagcttt tccaggaacc agacgtcctg caggagtgcg ggaccgcggg
     4021 gtccggcggt catgatgttc tggttgtcgg cgacgggggc gcctgcgttg gtggtgagct
     4081 tcttcttttc ggtcatggga attctcctcg ttttggggtg ttggaggtga aggacggccg
     4141 caaggaatcc gtcggtcaaa ggaccggagc aatcgagcgc aacccgcgag atcgctcaag
     4201 gaaaccggaa atgaaaatgg gaacttacaa aagccttgga tctatagaga agaattctcc
     4261 gtagaaaggc aaaaaaagag acctttagcg cctctcctgg cattcggggc agatgccgta
     4321 aaattcgagg cgatgactga gaattttgta ccctgtatcc gagaccagtt cccgggtgat
     4381 gtccgtgagg ccgcccagtt cgggatcgac gatctttttg cacctcgtgc agatgaggtg
     4441 gggatggggg tagggtttgt ttccgtcgta gcgattgtga tcgttgctga actccagttc
     4501 caacagctcc cccagttcct taaggacgga tatggttttg tagacggtgg caaggctggt
     4561 ggtgggaaag ttgaccttga ccttctcgaa gatgtcttcc acgctgggat gatcgcggct
     4621 ttccaacagc acccgcaata cggcgatgcg ctggggggtg atgcgatggc cctgttcccg
     4681 caacttatcg agcaaccgtt ccatgcggtc ctgcattgct tccacgtctc cggctccgga
     4741 cagtgtatcc aaatgttgta gagaattgtt atccgaaaag atccggttcg tcaagaccgg
     4801 aatgaacccc caggggaatt ctcacgcatc tccggacttt tccttccagt cttctttcca
     4861 atccggcagg gccctcgcca cggtcagggc cgtcacctcc ccggcaccgg cccgccgaag
     4921 actccgggcg cactcgttga gggtgctccc cgtggtgaag acatcgtcca ccaggagcac
     4981 gtgcaatcct ttcacatgct gaggattccc cacccggaaa gcgttcttca cgttggcatg
     5041 cctctgctcg cgatggaggc gggtctgggg atcggtccat tggaaccgat agagcaggtc
     5101 gtagcggacc ggccgatgga gccgggcgcc caggaatcga cccaagaggg cggcctggtt
     5161 gaagcctctt tgcctcaggc gccggacgtg gaggggcacc ggcacaatgc aatccgcctc
     5221 cgtcaagccc tctctgcggg ccgtctcgac cagcagctcc gacaacggag gtccccagtg
     5281 aagccgacct ccgaacttga actggtggat gcggtcccga accaccccgg aatgataggt
     5341 ggcggaccgg gcgcactgga agtggaactc tccccggagg cagttcccgc aaaggtggtc
     5401 cgaagaggag gggcttttgg gaaacggccg cccgcacaag gggcacttgg ggctgcgaat
     5461 ccaggggagt ccctcccggc agctcccgca ccagaatccg tctcgagatt ccagccaggt
     5521 ccgcccacat ccggcacagg aagcgggaca gaaaagctct cccagggcct gtccgattct
     5581 gcgggcaact tcccccaccc gggttctcat aaggaccctt tccgcatcca ccggttgaag
     5641 ccgtcccggg acaggcactc tctcccccgc ctcggcttcg agggatgagg gcggcatggc
     5701 tccccttggg agagggcgcc gggaggcctt cattcctgca tacgctccac cacagcccgg
     5761 gcgaattccg agcaccggac ctcccgggcc ttttccatca gacgcgccag gtcgtacgtg
     5821 acgatccttt ccaggatggt tcgctcgagg gcttgctcga ccagccgggc cgcctccctc
     5881 caccccaggt gctccaacat catgcagccc gaaaggatca gggagctggg attcaccttg
     5941 tccattcccg cgtacttggg agcgctgccg tgggtggcct cgaacacccc gcatcggtct
     6001 cccaggttgg ccccgggcgc catgcccagc cctccgacct gggccgccag ggcgtcggaa
     6061 aggtaatccc cgttcaggtt gggcgtcgcc accacgtgat attcctcggg cctcagaagg
     6121 acctgttgaa acatggaatc ggcaatgcgg tccttgacga cgatctttcc ctcgggcacc
     6181 acgcctccgt attcctcccg gacccgggtc tcggagacgg tctcccgggg aaattccgat
     6241 gcggccagtt cataccccca tttttgaaaa gccccttccg tgaacttcat gatgttgccc
     6301 ttgtgcacca gggtgaccga aggcaactcc ttctcgacgg cataccgaag agctcttcga
     6361 accaaccgct ccgttccgcg gcggctcatg ggcttaagac cgatggcgga ctcgggagca
     6421 atccgaatcc cgaattgttc ctggacgaag gagatgatct tttccgcctc ccgggaccgg
     6481 gcgggccatt c
//
LOCUS       DAOOVC010000192         6415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7037_length_6415_cov_6.626887, whole genome shotgun sequence.
ACCESSION   DAOOVC010000192 DAOOVC010000000
VERSION     DAOOVC010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7037_length_6415_cov_6.626887"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..538
                     /locus_tag="PLM79_16385"
     CDS             17..538
                     /locus_tag="PLM79_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOV87936.1"
                     /translation="MEEDVYVAPGAWIIGDVVIGSRSSVWFNTVIRGDEHYIRIGSET
                     NIQDNCSLHVTEGRYPLEIGNRVTVGHRAIVHGSVVEDECMIGMGSIVMDGCRIGRGS
                     LIAAGSVVTMGTVIPPHSLVVGTPGTVKKEITDKERRRMQESIAHYLQLGQDYLKPEL
                     LREKIRIKGFLGD"
     gene            complement(593..844)
                     /locus_tag="PLM79_16390"
     CDS             complement(593..844)
                     /locus_tag="PLM79_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87937.1"
                     /translation="MHMDRAIFDLKASVEATSLYILLCALLDQGEPTSIERARVQWNG
                     TEDGLIQAAKELMTRGVLETRNPLSEKTVLSLNPKGQWR"
     gene            complement(902..1237)
                     /locus_tag="PLM79_16395"
     CDS             complement(902..1237)
                     /locus_tag="PLM79_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87938.1"
                     /translation="MIELIKKSLYAGIGAAVLTKEKIQEMTRRLVEEGKITTEESEKL
                     ADEMVKSGERQWEEMSHGISEAMKRWTENLDVVRRKDLTLVEERIKLLEQRVSLLEAQ
                     DRKEGESNP"
     gene            1549..1833
                     /locus_tag="PLM79_16400"
     CDS             1549..1833
                     /locus_tag="PLM79_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOV87939.1"
                     /translation="MPTVRQSIVDLLCGGEFSAREVSQALGIREKEVFEHLSHIGRSV
                     ARRNKRLVVCPARCLQCAFVFVARGSYKKPGRCPVCKGEHIQDPRYTVRT"
     gene            1987..3099
                     /locus_tag="PLM79_16405"
     CDS             1987..3099
                     /locus_tag="PLM79_16405"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOV87940.1"
                     /translation="MAKVIIRRAGYDYPALRDHFFEMVESLAGDRIGPGGRVLIKPNL
                     LGPAVPDKAMVTHPMVVKAAVEYVLHKGARPQVSDSPAMGSWEKVLKESGISQALQGL
                     DVLCTPFKKSVSVDVGEPFHRIEIAEDALCADVVLNLPKLKTHSQMLLTLGVKNLFGC
                     IVGLRKPEWHFRTGVDREMFAYLLACICRKVNPTVTVLDGILAMEGQGPGKGGVARHL
                     GRLLAGTGAAAVDVAVCRILGLEEDRLLTNRAAKKLGLLREEVELDGDFRPVNDFKLP
                     GITPLVFGPACLHKTMRKHLVQRPVPASNLCKACGDCRRYCPAGAIQQKSKTLIFDYD
                     KCIRCYCCIEVCPYGALRAQEPLLGRVFTEIRNRLS"
     gene            3164..3526
                     /locus_tag="PLM79_16410"
     CDS             3164..3526
                     /locus_tag="PLM79_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87941.1"
                     /translation="MWPLREMRRSKRRSVDWSASLECIHEDLRQSVEVRVVEVCPEGV
                     RLVLKKMQVGPYHLFVGDVPTRLELTIPSPRGPITGEVRGRWYNWDESRGAFCMGAEF
                     VELSAEARTILGEDSSSS"
     gene            3674..4330
                     /locus_tag="PLM79_16415"
     CDS             3674..4330
                     /locus_tag="PLM79_16415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV87942.1"
                     /translation="MGYKAVIFDLDGTLLDTIRDLADTVNEVLKELDLDPHPIEAYKV
                     FVGDGMKNLVLRALPESLREEEALVERALSRTRAVYSERWRLHTRPYPGIPRLLDELS
                     ARKLHLAILSNKPDPSTRDVVREMLPRWSFRAVLGERPWVPRKPHPAGALEIAACLSL
                     PVSDILYVGDTGTDMKTANAAGMFAVGALWGFRTARELLDNGARTLIEKPLDLLSLLP
                     "
     gene            complement(4544..5563)
                     /locus_tag="PLM79_16420"
     CDS             complement(4544..5563)
                     /locus_tag="PLM79_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOV87943.1"
                     /translation="MKWDRSILVTGSAGFIGFHLAEHLLNSGNRVVGVDNLSDYYDVN
                     LKEDRLQLLSRSPNFTFRKLNLQDRSGMEKLFDQHSFDVVVHLAAQAGVRYSLKNPHA
                     YVESNLTGFIHVLEGCRHRQVPHLVFASSSSVYGANTSMPFSVHQNVDHPVSLYAATK
                     KANELMAHSYSHLYGLPCTGLRFFTVYGPWGRPDMALFIFTRAILAGRPIQVFNYGKM
                     RRDFTYIDDIVEGIVRVMAKAPEADPQWSGDSPDPGSSYAPYRIYNIGNHQPVELISF
                     IEALEECLGVKARKELLPLQPGDVPATYADIEDLSRVFGFRPKTSVREGISRFVEWYR
                     SYYGV"
     gene            complement(5855..>6415)
                     /locus_tag="PLM79_16425"
     CDS             complement(5855..>6415)
                     /locus_tag="PLM79_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV87944.1"
                     /translation="IFSSCSFCGLAPFRGRFIEKFIEKVFQEGMMQTSYFQNIAFCTD
                     FSENADEAFTVARDLAWRYGATLHIVHVMVNFSLSPPIHSSYLPIEYDPKFVEQVAEA
                     ARGSIQERYIGLLKEKQPYEIHLLSGYASTEILRLVKEKYVDLIVMGSHGLTGLAHVL
                     FGSTADRVVRKAECSVLTVRLKKPGT"
BASE COUNT         1260 a         1889 c         1985 g         1281 t
ORIGIN      
        1 taataagttt ccacgactgg aagaagatgt ctacgttgcc cccggggcgt ggattatcgg
       61 agacgtggtc atcgggagtc gatccagcgt gtggttcaat accgtgattc gaggggatga
      121 acattatatc cgcatcgggt ccgagaccaa catccaggac aactgcagcc tgcatgtgac
      181 cgaaggccga tatcctctcg agatcggcaa ccgggttacc gtcggtcaca gggccatcgt
      241 ccacgggagc gtggtcgaag acgaatgcat gatcggcatg ggttccatcg tcatggacgg
      301 gtgcaggatc ggcagaggat ccctcatcgc ggccggatcc gtggtcacca tgggaaccgt
      361 gattcccccc cattctttgg tggtgggaac ccccgggacc gtgaagaagg aaatcaccga
      421 taaggaacgc cggaggatgc aggagagcat tgctcactac ctgcaactgg gacaggatta
      481 cctgaagccg gaactcctga gggaaaaaat ccgcatcaag ggcttcctgg gggactgatt
      541 ctcccggaac ccgtctcgtc ggtcgccctg attcgggagg aacggctccc ttctagcgcc
      601 attggccttt ggggtttagg gaaaggaccg tcttttcgga tagtggattc cgggtttcga
      661 ggacgccgcg ggtcatgagc tccttcgcgg cctggattaa accgtcctcg gtcccgttcc
      721 actgtactcg ggcacgctcg atgctggtgg gttctccctg gtccaggagg gcgcaaagca
      781 gaatatagag agaagtagct tccacactgg ctttgaggtc gaagatcgca cgatccatgt
      841 gcatgaaata aggtctcctg agtcggtacg ccaacaagca gaggtcatca ctcgaccccg
      901 gttacgggtt ggattcgcct tccttcctgt cttgagcctc cagcaacgaa acacgctgtt
      961 cgagaagctt gatgcgctcc tccacgaggg tgaggtcttt tctgcgcacc acgtcaagat
     1021 tttccgtcca ccttttcatg gcttcggaaa tgccgtggct catttcctcc cactggcgct
     1081 ccccgctctt gaccatctcg tccgcgagct tctcggattc ttcggtggtg atcttgcctt
     1141 cttccaccaa ccggcgggtc atttcctgga tcttttcctt ggtgagcact gccgctccaa
     1201 ttcccgcgta gaggctcttc ttgatcagtt caatcatgac gatctccttc tcgggttgcc
     1261 cggggaatcc cggctgcatg ggtgcttgac cccatggttc cgcctccccc ccatgttccc
     1321 tatcaatccg acttcgctgg taaaaaatac cactcgcccc ctgatgaggg caagacaatg
     1381 tgccctggag ccgatctttt cctgcatctc ccgacgcaac ggtgctacta taatctacgg
     1441 ggaggctttg atccagccca tgtccgtggt ttattttgcg gtccgaggcg gtcctccaaa
     1501 agggagctgg gggccgaggg ccgaacggga accaacgagg gagcgggaat gccgaccgtt
     1561 cgccaatcca tcgtcgatct gctttgtggt ggggaattca gcgccaggga agtgtcccag
     1621 gctttgggga ttcgcgaaaa ggaggtcttc gagcacctga gtcacatcgg acggtccgtc
     1681 gcccgtcgga acaagaggct ggtcgtttgc ccggcccgct gcctccagtg cgcattcgtg
     1741 ttcgtcgctc gcggttccta caagaaaccc ggtcgatgtc ccgtgtgcaa gggcgagcac
     1801 atccaggacc cccggtacac ggttcgcacg tgaggctccc ggctgcccgg cgttcatgac
     1861 cgccgggatc tgccgaggag aacggggatt gcccacctcc acccactcgc cggggaggac
     1921 gccgcccccg gtccccccct gcaagccggg ccccacggcc ccgggagaag gacaagaagg
     1981 agctctatgg ccaaagtcat catccgccgg gcaggctatg actatcctgc cctgagagac
     2041 cattttttcg aaatggtgga atcccttgcg ggggaccgga tcgggccggg aggccgagtc
     2101 ctcatcaagc ccaacctcct gggccccgcc gtgccggaca aggccatggt cacccacccc
     2161 atggtggtga aggcggccgt cgagtacgtg ttgcataagg gggcccgccc ccaggtatcg
     2221 gacagtccgg ccatgggttc ctgggagaag gtgttgaagg aaagcggaat atcccaggcc
     2281 ctacaaggcc tggacgtcct ttgcaccccc tttaagaaat ccgtctccgt ggacgtgggg
     2341 gaacctttcc accgcatcga gatcgccgaa gacgccctgt gcgccgacgt ggtcctgaac
     2401 cttcccaagc tcaagaccca ttcccagatg ctcttgaccc tgggggtcaa gaaccttttc
     2461 ggctgtatcg tcgggcttcg caaacccgaa tggcactttc gcaccggtgt ggaccgcgag
     2521 atgtttgcct acctgctggc gtgcatttgc cggaaggtga acccgaccgt gacggtcttg
     2581 gacgggatcc tggccatgga aggtcaaggg ccgggcaaag gcggagtggc gcggcatctg
     2641 ggcaggctgc tggcggggac cggggctgcg gccgtggacg tggcggtctg caggattctg
     2701 ggcctcgaag aagatcgcct cttgaccaac cgggcggcaa agaagctggg gttgctccgg
     2761 gaagaagtgg agctcgacgg ggattttcgt cccgtcaacg acttcaagct gcccgggatc
     2821 acgcccctgg tgttcggccc cgcctgcctt cacaagacca tgcgcaaaca cctcgtgcag
     2881 cggcccgttc cggcctccaa cctgtgcaag gcctgcggcg actgcaggcg gtattgtcct
     2941 gcgggtgcca tccaacagaa atcaaaaacg ttgattttcg actacgacaa gtgcattcgt
     3001 tgctactgtt gtatcgaggt atgtccttac ggagccctcc gtgcccaaga gcccctgctg
     3061 gggcgggtct ttaccgagat caggaaccgg ctttcctaag gaccatccca tgagcgccca
     3121 aaggcgcccg ccgtgaagca acgccgggca tggaggcaaa accatgtggc ccctacgtga
     3181 aatgagacgc tccaagcggc gatccgtgga ttggagcgcc tccctcgagt gcattcacga
     3241 ggacctgcgg caatccgtcg aggtcagggt ggtggaagtc tgtcccgaag gcgtacgcct
     3301 ggttctcaag aagatgcagg tcggtcccta ccatctcttt gtcggggacg ttcccacccg
     3361 gctggagttg accatcccgt ctcctcgcgg accaatcacc ggcgaggtcc gcggccgatg
     3421 gtacaattgg gacgagtccc ggggcgcctt ttgcatgggg gcggaattcg tggagctgtc
     3481 ggcggaagcc cggacaatcc tgggggagga ctcctcaagc tcctgatccg ggaccgtcgg
     3541 ggaggctgcc gggcagggtg ccttttcccg gccccgacgg gccggggatg aagatcgggc
     3601 gtcagcgtgt tgtgccgacc gatttgggat cggaaggatt ttcaaggaaa cccactcgga
     3661 acgagaaagc acgatggggt acaaggcagt aattttcgac ctggacggca ctctgctgga
     3721 caccatccgg gacctggcgg ataccgtgaa cgaggtattg aaagaactcg acctggatcc
     3781 tcatcccatc gaagcctaca aggtttttgt gggcgacggg atgaagaatc tggttttgcg
     3841 ggccctcccc gaatccctgc gggaggagga ggccttggtg gaaagggctc tttcccggac
     3901 ccgggccgtt tacagcgagc gatggaggct ccacacccgc ccatacccgg gaatcccccg
     3961 gctgttggat gagctctcag cccgcaaatt gcatctggcg atcttgtcca acaaaccgga
     4021 tccgtccacc cgggacgtgg tccgggagat gctcccccga tggagctttc gggccgtctt
     4081 gggcgagagg ccctgggtac cccggaagcc gcatcccgcc ggtgcactgg aaatcgcggc
     4141 gtgcttgtcc ttgccggttt cggatattct ttacgtggga gacacgggga cggacatgaa
     4201 aaccgccaac gcggcgggaa tgtttgcggt gggcgccctg tgggggttcc gaacggctcg
     4261 ggagctcctg gacaacgggg cccggaccct catcgaaaag cccctggacc ttctttccct
     4321 gctcccctga cgggaccgac cgggaacccg gccggtcttc gggactcgaa tcgagcgatc
     4381 tcatgacccg ggtcgcaaag agcgcaaacc ttcccgagcc ggagcgtgat ctcctccctt
     4441 tgagggggac cttctttggg ggacggactc gaccccccca agggaccccg acggtcttcg
     4501 aatccctcgt cccgggcggg aatccccctc gggttgcctc cgactagacc ccgtaatagg
     4561 aacggtacca ttctacaaac cgggaaattc cctcgcggac ggaggtcttc gggcgaaagc
     4621 cgaacaccct gctcaggtct tcgatgtcgg cgtaggtcgc cggtacgtcc ccgggctgga
     4681 gaggcagcag ctccttgcgg gccttgacgc ccaggcactc ctccagggcc tcgataaaag
     4741 agatcagctc cacgggctga tggttgccga tgttgtagat tcggtaggga gcatagctgc
     4801 tgcccggatc gggggagtcg cccgaccatt gaggatcggc ctcgggggct ttggccatta
     4861 cgcggacaat cccttccacg atgtcgtcaa tgtaagtgaa atcccgccgc atttttccgt
     4921 agttgaagac ctggatgggt ctcccggcga ggatggcccg ggtgaagata aagagagcca
     4981 tgtccgggcg gccccagggg ccgtagaccg taaaaaaccg gagccccgtg cagggaagac
     5041 cgtagaggtg cgagtaggaa tgggccatca gttcgttggc cttcttggtg gcggcataga
     5101 gggagaccgg atggtcgacg ttctggtgga cggaaaaagg catggaagta ttggccccgt
     5161 agacggagct cgacgaggcg aacaccaggt ggggaacctg ccggtgcctg cagccctcca
     5221 agacatggat gaagcccgtg aggttgcttt ccacgtaggc gtgagggttt ttcagggaat
     5281 aacggactcc ggcctgggcc gccaagtgga ccaccacgtc gaaggaatgt tggtcgaaaa
     5341 gcttttccat ccccgaccga tcctgcaggt tcagcttgcg gaaggtgaag ttcggggatc
     5401 gagacagcag ctgcagccga tcttccttga ggttgacgtc gtagtagtcg ctgaggttgt
     5461 ccacgcccac aacccggttt cccgaattca gcaggtgctc agccaggtga aatccgatga
     5521 atcccgcaga acccgtcacc agtatcgatc gatcccattt cacgtcggca acattccttt
     5581 tttcttttcg gcatttcgga agccggattg gagtgagagg aggaatccgc gccgggccgc
     5641 gatcctcgaa agaacgtccc atgggcgggg aatgccggtt cgaggcatcc cggtcgaggg
     5701 cctgaaagga gtccgttcgc tcgtttccga acgtggttcc ggattccttt ggagagtcgt
     5761 gccccgtccc ccggatccgt tcccatgaac acggcaccat cgtttgtaag acacaagggg
     5821 agaatccccg gagggcaggt cggcaggccg gaccctaagt gcccggtttc ttcaggcgga
     5881 cggtgaggac cgagcactcg gccttcctga ccacgcggtc cgcagtgctt ccaaagagaa
     5941 cgtgggcaag gccggtgagg ccgtgggacc ccatcacgat caggtccacg tatttttcct
     6001 tcaccagtcg caggatctct gtggatgcat agcctgaaag caaatggatc tcgtaaggct
     6061 gcttttcctt gagcaggcca atgtagcgtt cctggatgga tcctcttgcc gcttcggcca
     6121 cctgttccac gaacttcggg tcgtactcga tgggcaggta ggacgaatga atgggaggcg
     6181 acagagagaa attgaccatg acgtgcacga tgtggagggt tgcaccatag cgccaggcca
     6241 ggtcccgggc cacggtgaaa gcctcgtccg cattctcgga aaaatccgtg cagaatgcga
     6301 tgttttgaaa atagcttgtc tgcatcattc cctcctggaa aaccttctcg atgaacttct
     6361 cgatgaatcg accccgaaac ggcgcgagcc cgcaaaatga acaggaagag aatat
//
LOCUS       DAOOVC010000193         6400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7056_length_6400_cov_6.707801, whole genome shotgun sequence.
ACCESSION   DAOOVC010000193 DAOOVC010000000
VERSION     DAOOVC010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7056_length_6400_cov_6.707801"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLM79_16430"
     CDS             <1..763
                     /locus_tag="PLM79_16430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699430.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOV87945.1"
                     /translation="RDRGRHALIIYDDLSKQAAAYRQVSLLLRRPPAREAYPGDIFYN
                     HSRLLERAAKLNQELGGGSLTALPVIETQAGDVSAYIPTNVISITDGQIYLEPGLFFA
                     GVRPAINVGLSVSRVGGAAQTKAMKQVAGRLRLDLAQYRELEAFAKFGSDLDKATQAQ
                     LNRGMRLVELLKQPQYKPVPAEKQVTALYAGTRGFLDSVPVEKVREYETQMIAFMERK
                     HPDILAEIKEKNVISDALDGKLKQALQEFAEVFQA"
     gene            803..1675
                     /gene="atpG"
                     /locus_tag="PLM79_16435"
     CDS             803..1675
                     /gene="atpG"
                     /locus_tag="PLM79_16435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOV87946.1"
                     /translation="MATLRDIKRKIDAVKKTSQITKAMNMVAAAKLRGAQANMEKFHP
                     YAQKFNEVVKRLASGVEDASSYMLITPREEVKKVELVLLTADRGLCGSFNNNLIVRAE
                     RFMREKQAAGISVSLTAAGRKGADYFRRRTFEVRKRLTGLLNKPSFSDAQELGQDLIR
                     NFEIGTCDEVYVIYSHFVNMVKQVPTLVQILPITPEKEEETKGYFEYLFEPSHQALLT
                     DLLPTYVFIQLLEAFYQTSVSEHAARMAAMENATNNCKDLVRSLTLTYNKARQAGITK
                     ELMDIVGGAEALKK"
     gene            1717..3126
                     /gene="atpD"
                     /locus_tag="PLM79_16440"
     CDS             1717..3126
                     /gene="atpD"
                     /locus_tag="PLM79_16440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOV87947.1"
                     /translation="MNMGRIVQVIGNVVDVEFEEGKLPPLLTALFVSNPGISDEEDNL
                     VLEVAQHLGDNVVRTIGMDLTDGLIRGMMVKDSGKPIQMPVGKGVLGRVINVVGRAVD
                     GLGKIDTDKFMPIHRPAPAFTEQDTSVKVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMIHNIAMQHGGISVFAGVGERTREGNDLYHEMKDSGVLPRAGLVYGQMTEP
                     PGARARVALSALTVAEYFRDVEGQDVLLFVDNIFRFTQAGAEVSALLGRMPSAVGYQP
                     TLGTDLGELQERITSTTKGSITSVQCVYVPADDLTDPAPATTFAHLDGTVVLSRQIAE
                     LGIYPAVDPLDSTSRILDPNVLGEEHYYTSREVQQILQKYKDLQDIIAILGMDELSDE
                     DKITVSRARKIQRFLSQPFHVAETFTGVAGKYVKMEDTIRGFKEICDGKHDDVPEQAF
                     YMVGSIEEALEKAKQMAAA"
     gene            3147..3551
                     /locus_tag="PLM79_16445"
     CDS             3147..3551
                     /locus_tag="PLM79_16445"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOV87948.1"
                     /translation="MAEKILLEIVTPEKKVLSEQVDIVVAPGEEGEFGVLPNHIPFLT
                     KLKIGELRFRIGANARYVAIMGGFAEVLPDHVTILATAAEEAADIDVVRAKAARERAE
                     ARLREAKDRLEFARAEAALQRAMARLKIAEKA"
     gene            3705..5537
                     /gene="glmS"
                     /locus_tag="PLM79_16450"
     CDS             3705..5537
                     /gene="glmS"
                     /locus_tag="PLM79_16450"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOV87949.1"
                     /translation="MCGIVGYIGAEDGVPLLMEGLKRLEYRGYDSAGIAVINGQSRLQ
                     VVRCRGKIASLEARLREEPMEGTVGIAHTRWATHGKPCDENAHPHRSGPFVVVHNGII
                     ENYSEIKQDLTGKGCFFSSETDTEVIVRLADFEWRSGKEPVKAIRDALRQLRGSYAVV
                     FLNELNPDELIASRNESPLILGLGKGAYYLASDVPAILPYTREVLYLEDGDIVLLRRD
                     GFIIESMDGTRQERKVQRINWNPVMAEKAGYKHFMLKEIYEQPRAVIDTFRSVVDLNE
                     RKVVLGDLNLDARDLEKIQKIFLVACGTSYHACLMGKYVIEKMCRIPVEVDLGSEFRY
                     RMPLMDERTLLFVVSQSGETADTRAALREGLERGAVCRAIVNVVGSSLARMADGVIYT
                     HAGPEIGVASTKAFTTQMVALYLLSLYFAEKLGRMDPGTLSFHLQELIELPRKLELLL
                     GRNEEILGWAKRFSHARDFLYLGRGALFPIALEGALKLKEISYIHAEGYAAGEMKHGP
                     IALIDEKMPVVVLLQQGPLYEKMLSNVQEVKAREGAILALVEGDKDEKIGPAESQIIV
                     PGSSEWLSPILFSIPLQLLAYHIAVLRGTDVDQPRNLAKSVTVE"
     gene            6025..6203
                     /gene="ssrS"
                     /locus_tag="PLM79_16455"
     ncRNA           6025..6203
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLM79_16455"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         1446 a         1680 c         2010 g         1264 t
ORIGIN      
        1 ccgggatcgc gggcgccacg cgctcatcat ctacgacgac ctgtccaaac aggcagcggc
       61 ctatcgtcag gtttccctgc ttttgaggcg ccccccggca cgcgaggctt accccgggga
      121 cattttctac aaccactctc ggcttttgga acgagccgcc aagctgaacc aggagctcgg
      181 aggagggtcc ctgacggccc ttccggtaat cgaaacccag gcaggggatg tgtccgcata
      241 catcccgacc aatgtgatct ccattaccga cggtcagatc tacctggagc cgggtctttt
      301 ctttgccggg gtccgtccgg ccatcaacgt agggctttcc gtgtcccgag tgggcggcgc
      361 cgcgcagacc aaggccatga agcaagtggc gggtcgcctg cgtttggacc tcgcccagta
      421 tcgggagctc gaggccttcg caaaattcgg gagcgatctg gacaaggcta cccaggccca
      481 gctcaaccgc ggcatgcgct tggtggaatt gctcaaacag ccgcaataca agcccgttcc
      541 cgcagaaaag caggtcacgg ccctctacgc cggcacccga ggatttctgg acagcgtgcc
      601 cgtagaaaag gtcagggaat acgagacgca gatgatagcg ttcatggaac gaaagcatcc
      661 ggacatcttg gccgagatca aagagaagaa cgtcatcagc gatgcgttgg atgggaagct
      721 gaagcaggct ctgcaggagt ttgccgaggt ctttcaggca taatggcgtc aggacgaacc
      781 gagagatgag ggagcaggga acatggcgac cctgagggat atcaaaagaa aaatcgacgc
      841 cgtcaagaag acctcgcaaa tcaccaaagc catgaacatg gtggccgcgg ccaaactgcg
      901 cggggcgcag gcgaacatgg agaagtttca tccgtatgcc cagaaattca acgaggtcgt
      961 gaagagattg gcctcagggg tggaggatgc gagctcctat atgctcatta cgcccaggga
     1021 agaggtgaaa aaggttgagc tggtcctgct gacggcggac cggggtctct gcgggagctt
     1081 caacaacaac ctgattgttc gtgcagagag attcatgcgg gaaaaacagg cggctgggat
     1141 cagcgtctcc cttaccgctg cgggccgaaa gggggccgac tactttcgac gccgcacctt
     1201 cgaagtgaga aagagactga cggggctatt gaacaaaccg agcttcagcg acgcccagga
     1261 attgggccag gatctcatcc ggaatttcga gatcggcacc tgcgatgagg tttatgtgat
     1321 ctacagccat ttcgtgaata tggtgaaaca ggtgccgacc ctggtgcaaa tccttcccat
     1381 cacgccggag aaggaagaag agaccaaggg atacttcgag tatcttttcg aaccgtccca
     1441 ccaggcactg ttgaccgacc ttttgccaac ctacgtgttc atccagctcc tggaagcctt
     1501 ttatcagacg agtgtgagtg agcatgcggc tcggatggcc gccatggaaa atgccaccaa
     1561 caactgtaaa gatctggtgc gttccctgac cttgacctac aacaaggcac ggcaggcggg
     1621 cattaccaaa gagctcatgg acattgtcgg gggtgcggaa gctctgaaaa aatagaagga
     1681 atcggggtcc ccaggagtca aggaggtata tgacccatga atatgggaag aatcgtacag
     1741 gtgatcggaa atgttgtgga cgtcgaattc gaagagggaa agcttcctcc tctgttgacg
     1801 gcccttttcg taagcaatcc cggaatcagt gacgaggagg acaacctggt cctggaagtg
     1861 gcccaacatc tgggagataa cgtcgtgcgc accatcggca tggacctcac cgacgggctg
     1921 attcgcggga tgatggtcaa ggattcgggg aagccgatcc aaatgcccgt aggcaagggg
     1981 gtgctgggtc gagtcatcaa cgtggtgggc cgagccgtgg acggtttggg gaagattgac
     2041 acggacaagt tcatgcccat ccatcgaccg gctcctgcct tcaccgagca ggacacctcg
     2101 gtgaaggtcc tggaaacggg ggtcaaggtc atcgacctgc tggtgccgtt tccacgaggg
     2161 ggcaaaatgg ggatgttcgg aggcgccggg gtgggaaaga ccgtcgtcat gatggagatg
     2221 atccacaaca tcgccatgca gcacggagga atttccgtct tcgccggtgt gggggaaaga
     2281 acccgcgaag gaaacgacct ttaccacgaa atgaaggact cgggagttct gccgcgagcg
     2341 ggtctcgtgt acggccagat gacggaaccc ccgggggccc gggcgcgggt ggccttgtcg
     2401 gccctcaccg tggccgagta ctttcgggat gtggaaggac aggacgtgct cctcttcgtg
     2461 gacaatatct tccggttcac ccaggcgggt gccgaggttt cagcccttct gggccgcatg
     2521 ccgtcggccg tcggctacca gcctaccctg ggaacggacc tgggcgaatt gcaggagcgg
     2581 atcacctcca ccaccaaagg gtccattacc tccgtccagt gcgtgtatgt gcccgcggac
     2641 gacctgaccg acccggcccc ggccacaact ttcgcacact tggacggcac ggtggtgctt
     2701 tcccgccaga ttgcggagct ggggatctat ccggcggtgg atccgttgga ttccacatcg
     2761 cgaatcctcg atcccaacgt cctcggagaa gaacattatt acacctcccg ggaggtccag
     2821 cagatcctgc agaaatacaa ggatctccag gacatcattg ccatcctggg catggatgag
     2881 ctgtccgacg aggataagat tacggtgtcg cgagctcgta agatacaaag attcctgtcc
     2941 cagcctttcc acgtggcgga gacctttacc ggcgtggcgg gtaaatacgt gaagatggaa
     3001 gacaccattc gagggttcaa ggaaatttgc gacgggaagc acgatgatgt tcccgagcag
     3061 gccttctaca tggttgggtc catcgaggaa gctttggaga aagccaagca aatggctgct
     3121 gcttaaaggg aaaagatggt gggcttatgg ccgagaaaat cctgctggag atagtcaccc
     3181 ccgaaaagaa ggtgctcagc gaacaggtgg acattgtcgt ggcccctgga gaggaggggg
     3241 agtttggagt cttgccgaac catattccct ttctcacaaa gctgaagatt ggagagctac
     3301 gcttccgaat tggcgcaaat gcacgatacg tggcgatcat gggcggcttt gcggaagtgc
     3361 tgccggacca tgtgacgatc cttgccaccg ccgccgagga ggctgccgac attgatgtgg
     3421 ttcgagccaa agcggcgagg gaaagggccg aggcgaggct gcgcgaagcc aaggatcgcc
     3481 tggaattcgc ccgggcggaa gcagcgctgc aaagggccat ggcacgattg aagatcgccg
     3541 aaaaagcctg atcctttctt tggaaggaac ccacgaaaag cccttctctt ctaggaaggg
     3601 cttttcgttt ttctttacac ttcctcatgc gggtttcata atgattcccg ctcctgggag
     3661 cagagtgccg gaggagctgt cgggatctgg gaggagaatc gtgtatgtgc gggattgtcg
     3721 gctatatcgg ggcggaggac ggggtgcctc ttctcatgga ggggttgaag cgcttggaat
     3781 accgcgggta tgacagtgcg ggcatcgccg tcatcaatgg tcaaagtcgt cttcaagtgg
     3841 tgcggtgcag agggaaaatc gcatccctgg aggcaaggct tcgggaagag cccatggagg
     3901 gaaccgtggg catcgcccac actcggtggg ccactcatgg gaagccttgc gacgagaatg
     3961 cgcatcccca tcggtccggc ccgtttgtgg tggttcacaa cgggatcatc gaaaactact
     4021 cggagatcaa gcaggacctt acgggaaaag gctgcttctt ttcatcggag acggacacgg
     4081 aggtcatcgt acgactggcg gactttgaat ggagatcggg aaaggaacct gtgaaggcca
     4141 ttcgagacgc tctgcgacag ttgagaggga gctacgcggt cgtgtttctc aacgagctca
     4201 accccgacga gctcatcgcc tctcgaaacg aaagcccgct gatcctgggt ctgggaaaag
     4261 gagcttatta tcttgcctcc gacgtgcccg ccatcctgcc ctacacccga gaagtcctgt
     4321 acctggaaga cggagacatc gtccttctca ggagggacgg attcatcatc gagtccatgg
     4381 acggaaccag gcaggagcgc aaggtccaga gaatcaattg gaatcccgtg atggccgaga
     4441 aggcgggcta caagcatttc atgctcaaag aaatctacga gcagcctcgg gccgtcatcg
     4501 acacctttcg cagcgtagtg gacctcaacg aaagaaaggt cgtcctgggt gacttgaatc
     4561 tggatgcccg cgaccttgaa aaaatccaaa agatcttcct cgtcgcctgc gggacttcgt
     4621 atcatgcgtg cttgatgggc aaatacgtca tcgaaaagat gtgtcgaatt cccgtcgagg
     4681 tggatcttgg gtcggaattc cgatacagga tgccgttgat ggacgaacga accctgctct
     4741 ttgtggtgag tcaatcgggg gagaccgcgg atactagggc ggccctgagg gagggactgg
     4801 aacggggggc ggtgtgcaga gccatcgtga atgttgtggg aagcagcctg gcccgaatgg
     4861 ccgacggcgt catctatacc cacgccggac cggagatcgg agtcgcctcg accaaggcct
     4921 tcacgactca gatggtggcc ctttacctgc ttagcttgta ctttgcggag aagctgggcc
     4981 ggatggaccc ggggaccctc tcgtttcacc tccaggaact catcgagctc ccacggaagc
     5041 tggagctgct tctgggacga aacgaggaga tcctcggttg ggcgaagaga ttttcccatg
     5101 cccgggattt cctctacctg ggtaggggcg cactgtttcc catcgccctg gaaggcgccc
     5161 tcaagctcaa ggagatctct tacatccatg cggaagggta cgcggcgggc gagatgaaac
     5221 acgggcccat tgcgttgatc gatgagaaga tgccggtggt agtcttgctg cagcaggggc
     5281 cgctctacga gaagatgttg agcaacgtgc aggaagtgaa ggcgcgagag ggtgcaatcc
     5341 tggcattggt ggagggggac aaggacgaaa agatcggccc cgcggagagt cagatcattg
     5401 tgcccggatc ctccgaatgg ctctccccca tactcttctc cattccgctg cagttgctcg
     5461 cctaccatat tgcggtgctc cggggcacgg acgtggatca gcccaggaat ttggccaaga
     5521 gcgtcacggt cgagtgagga gcaggaaata cttgggagat tgaacttgac ttgtattttc
     5581 ctttttgact agaatgttta cggagttcga aatcttttgt gattatgggt gtttggcata
     5641 tcgttgccct gtgcggcagg ggcgccctga gggcccgatg cctcggaggg attccgccgg
     5701 aacatagcaa caatcggaag tgggagaacc tggggaaagg ctcatcatct tcatatagtg
     5761 tccggatcag gttgcggaga gagaaaagaa acggtacgac gaaacgagga cacgtggtcc
     5821 ggtttcgaag gggcggttgc ttcgcttatg cggttcccgc cggggggagg acctgggagc
     5881 atttacgggg ccgagggaag cctcccaggg gggagcgcgg agcaatgcag cacataataa
     5941 aggtctgcga gcggattgct cgtcgttttt tcggcaaagg gagcgggacc ttcgggggac
     6001 cgctcggtgc tcggccgagg tgtatggccc ctgctgtgtt tgtgatcgat agaacgttct
     6061 ggagccaaca gaaagcagta tgggaaccct ctcttggtcc ggtgcgcaag tcacgccgtg
     6121 aattgcctaa aagatcaacg gggtgcccac ctaggtcgtc cggttcaaga ccgactatcg
     6181 acacggcatg acgcgggggc tccttcgagc tgtttctctc caattcctgc cgtctcaacg
     6241 cgtccttcct ccgagctttt cttgcgtttg ctacactcct aatgttgccg aacgttgcca
     6301 aaagggagcg ctcccggatt ggttcgccga cgagtctttc cctgcaacgg aacaccgacg
     6361 acctctcggg ggtcaaacag gaccgctggg gtcgggcaac
//
LOCUS       DAOOVC010000194         6331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7136_length_6331_cov_5.782027, whole genome shotgun sequence.
ACCESSION   DAOOVC010000194 DAOOVC010000000
VERSION     DAOOVC010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7136_length_6331_cov_5.782027"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..3071)
                     /locus_tag="PLM79_16460"
     CDS             complement(276..3071)
                     /locus_tag="PLM79_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOV87950.1"
                     /translation="MDNLEIQEHLLDLLESGYEVLTESERFAHQLQRRFRSRRMRAAC
                     PGWTSPAAGPLNHWLERFWKSLWPEDWPAPPFLRWRLLSQTVVEVPPPAPLSPGPLLV
                     RDLDESFELCLRYGIDPGSADAPTPLVDWRSRVWLSCRRELEKQGLFYPALLPRKILS
                     VLERDPKRAPLGIAMVGFEFAGHWEKALIGALSSLPGNRHLPLPSPETENRALVYSDP
                     QQEVMGLLEHLARAAEEVPLHSLAVVVLDAPGYSPLLAGGLQDLFGLPLEGDQAAYNL
                     FPEPRLTDQSLYHAALLPLTFAHEGESRQRFTGFLRSPYYAFTARHTRALSQWDRYWR
                     EKGIEGGLEDLTQPVPPFLEDVLPGNGRELRARFRPLMGPHQRPGSHWVRDLKAFWEG
                     FGFPLLAHEIDQIAWDCLQRILDQFEAAFGETPMTGREFTEWLQGAGEKIHVQRRGLE
                     DAGIQVLGGLEIRGLSFQRVYVPALMGKVLPQPVRSFPFLTPHERRRVQGGTAESQFQ
                     FAGHLFAQFHAAAPRVMLSRPLLGLGGDPEPPSPFWPDDREEKQPTRVPWRHFLPGIQ
                     RARWVRAGIAGMKAHQGALTECPAHASVSEEGGKGADEDEFQGDPIRIRRSPQDLSYV
                     GPLSLPEELTASAVEVLVSCPARFFFEAILKIAPLPETGRGVDPRQRGGIIHRILAVF
                     GEMLMCRRRETGGDPERPENILARVVDRELKSLLSIPSWQIEKKRLLGKDDRAPGLLT
                     EWINREMVYLEQGWRWQAFEVPFGGLRLRDCPVSICGRVDRVDIHPDQGIVCWDYKTG
                     RIPTRKEVWEEVVSPQVPIYLMALRQGLIPGVPPDPPSLNGGYIDLRSVRHLDRKLIA
                     QGDRPLDPLLLDHEQKISRLVRDVLEGHIRGRWTEGSCEVSCPYECLCGLRLFQEDSP
                     SPSPSRAPHATPDHQVP"
     gene            3304..5619
                     /locus_tag="PLM79_16465"
     CDS             3304..5619
                     /locus_tag="PLM79_16465"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HOV87951.1"
                     /translation="MKNVFSNVLECIGNTPLILINRMNPNPKVRLYVKWEARNPGGSI
                     KDRPALCMIECAEREGVLTPDKTIIEATSGNTGIGLAMVAAVKGYRIMLVMPETASLE
                     RQKVLKALGAELLLTPGSLGTDGAIEEVYSLVRKDPDRYFMPDQFSNPANPLAHYLGT
                     GPEIYEQTGGKVDAVVITLGTTGTAMGILKAMKDRNPSIRVIGVEPYPGHKVQGLKNM
                     KESYVPAIFDRHAMDRILPVRDEDAFEMARRLAREEGIFAGMSSGAAMAAAVQVAAEM
                     EEGTLVAVLPDGGDRYLSTNLFTTLLEPDFHFFDLLRREKTEFKPVQEGKARLFVTSP
                     PLDQPLTLQEARRFVLADLLTRFLKAKGFSVDQMILIPDLESRTIQGAISTGMDLAAY
                     SQRQMDEVLRDLDRLGVQRARRYPRITDHLDTIPEHTQALIDRELAYEKLRSVYFNLS
                     RSRNYGALSRVDPKKIRVGATVDLDAYEKLNPRDFTLLKRATLTELKHGIYVKTSWGN
                     VVPTWHISAASIALHDLGAPIDIVVSSVDFLFPHLENVREIGEALTGKLFAKVWMVCE
                     RIWSSKEEKGSDQADENTPVRSLTASGTSPDQLRYWFLCTHYRKPIHFTPANLRNTLH
                     GYARLQEFIRRVQYVSCRNEEHKNMGEFLYALEQGFFDALADDLNTPKALAAMFRFTR
                     GVNPILDSGQFSENQRKQILETFQKLNGILGILDLDLEALSPEEEELVKARHEARASK
                     DWAKADLLRRELLDRGIQVVDTPAGTRWTRI"
     gene            complement(6011..>6331)
                     /locus_tag="PLM79_16470"
     CDS             complement(6011..>6331)
                     /locus_tag="PLM79_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87952.1"
                     /translation="TLWQNISLGLAMNQNGNALTTENREYIKRPYRGIPDELWQEMWA
                     WARETKNTGGNIKNVNQPFAARMNAIPLQCKRRITEHAYEEALRLFEATHSIGLAEGV
                     LELL"
BASE COUNT         1262 a         1976 c         1906 g         1187 t
ORIGIN      
        1 ttccaggatg gcctgacgat gcttccctct ggccacgaaa tggatgtccg tccctgctcg
       61 tgccaagagc cccccaaaat aaccaccgat gcccccggaa cccattacgc cgattttcat
      121 gaagtgctcc tcttgtggca aagttgagcg ttgacctgcc tggcgactat cttggacaac
      181 tttgggcctt tatacctgca tgggtccccc aggtcaaccg gccttccccg ccctcggatt
      241 cccacggact tccccacccg agccggcgtg gcggttcacg ggacttgatg atccggcgtg
      301 gcgtgcgggg cacgggaggg ggagggggac ggggagtctt cctggaagag acgaagcccg
      361 cacagacact cgtagggaca ggaaacctcg caggagccct cggtccatcg tcccctgatg
      421 tgcccctcca aaacatcccg gacaagacgg gagatctttt gctcatgatc gaggagcagc
      481 ggatcgaggg gcctgtctcc ctgtgcgatg agtttccggt ccaggtgacg gaccgaacga
      541 agatcaatgt atccgccgtt gagagacggg ggatccgggg ggacaccggg gatgaggccc
      601 tgtcggagag ccatcaggta aatgggcacc tggggagaaa ccacctcttc ccacacttcc
      661 ttcctggttg gaattctccc cgtcttgtag tcccagcaga caatgccctg atccgggtga
      721 atgtccaccc ggtccacccg gccgcaaatg gagacggggc aatctcggag cctcagtccg
      781 ccaaaaggga cctcaaacgc ctgccatcgc catccctgct ccaggtagac catctcccgg
      841 ttgatccatt ccgtgaggag tccgggcgca cggtcgtctt ttcccagcag cctcttcttt
      901 tcgatttgcc aggacgggat ggacagcagg gatttcagct ccctgtcgac gacccgggca
      961 aggatgtttt ccggtcgctc ggggtccccg cccgtctctc gcctgcggca catgagcatt
     1021 tctccgaata ccgcgagaat cctgtggatg atcccgcccc tctgccgcgg gtccacccct
     1081 cttcccgtct cgggcaacgg ggcgattttg agaatcgctt cgaagaagaa gcgcgccgga
     1141 caggaaacga ggacctccac ggcactggcg gtcagttctt ccggaaggga taggggcccg
     1201 acataggaaa gatcttgggg agaacggcgg atgcggatcg gatccccttg gaattcgtcc
     1261 tcgtcggctc ccttacctcc ttcctcggaa acggacgcat gggcgggaca ttccgtgagg
     1321 gcaccctgat gtgccttcat cccggcgatg cccgccctca cccatcgagc ccgttggatt
     1381 cccggcagga aatgtctcca ggggacccgg gtgggctgct tctcctctct gtcgtcgggc
     1441 cagaacgggg aaggaggttc cggatctccg cccaggccga ggaggggccg ggacaacatg
     1501 actcggggag ccgccgcatg aaactgggca aacaggtggc cggcgaattg gaactggctt
     1561 tccgccgttc ccccctgaac ccgccttcgt tcgtggggag tgaggaaggg aaaggatcga
     1621 accggctgag gcaggacctt tcccatgagg gccggaacgt agacgcgttg gaaactgagc
     1681 ccacggattt cgagtccccc gaggacttga atcccggcat cttccaggcc ccggcgttga
     1741 acgtggatct tctcaccggc tccttgaagc cactcggtga attcgcgtcc cgtcatggga
     1801 gtttcgccga aggccgcttc aaactggtcc agaattcgtt gcagacaatc ccatgcaatt
     1861 tggtcgattt catgggcaag gagggggaac ccgaaccctt cccagaatgc tttgaggtcc
     1921 ctcacccaat gagagcctgg ccgttggtgc ggtcccatga gcggccggaa ccgggctctg
     1981 agttctcggc cgtttcccgg aagcacgtcc tcgaggaaag gaggcaccgg ttgggtcagg
     2041 tcttccagcc ccccctcgat ccccttttcc ctccagtacc ggtcccactg gctgagagca
     2101 cgggtgtggc gagccgtgaa tgcatagtag ggggagcgca gaaatcccgt gaaccgttgc
     2161 ctcgactcgc cttcatgggc gaaggtcagg ggcaggagcg ccgcatggta tagggactgg
     2221 tccgtgagcc ggggttccgg aaacaggttg tacgctgcct ggtccccctc gagggggagc
     2281 ccgaacaggt cctgaagacc cccggcaaga agggggctgt aaccgggagc gtccaggacc
     2341 accacggcca aggaatggag ggggacctct tcggccgccc gagccaggtg ctcgagcaat
     2401 cccatgacct cttgctgcgg atccgagtat accaaggccc tgttttcggt ttcaggagac
     2461 ggcaaaggaa gatgccgatt cccgggcaga ctgctcaggg ccccgatcaa ggccttttcc
     2521 caatgaccgg caaactcgaa cccaaccatg gcaatcccga ggggagcccg tttcgggtcg
     2581 cgctccaaaa cggacaggat cttccggggg agaagggccg ggtagaacag tccctgcttc
     2641 tcgagttccc gccgacagga gagccagacc cggcttctcc agtctaccag gggagtcgga
     2701 gcatccgcgg agccgggatc gattccgtag cgcaaacaga gttcaaagct ttcatccagg
     2761 tcgcgcacca ggagaggacc gggagagagg ggggcgggcg gagggacctc gacgacggtt
     2821 tgagaaagca ggcgccatcg cagaaaagga ggggccggcc aatcctcggg ccagaggctc
     2881 ttccagaacc gttccagcca atgattcagg gggcccgcgg ccggagaggt ccatccgggg
     2941 caggccgccc tcatcctgcg gctgcgaaac cgcctttgga gttgatgagc aaacctctcg
     3001 ctttccgtga ggacctcgta gccggattcg agaaggtcca gcaggtgttc ctggatctcc
     3061 aggttgtcca ttccgtcatc cattcaggca aacggcatcc agttggtctt tccgctgcgg
     3121 caccgcttta tataacacgg ggaaacactt tgacaaaaat tccttcggct gcctatatac
     3181 agggtcggga atttccccat cgagcatggg gaactcccgg tttgcccgaa ttcatcccgg
     3241 ggatttcggc gggctcccgt gccggaagga ccgctttccc ccagagagga cccggacccg
     3301 accatgaaga acgtcttttc caacgttttg gaatgcattg ggaatacgcc gctcattctc
     3361 atcaaccgaa tgaatccgaa cccgaaggtt cgtctttatg tgaagtggga agcgagaaac
     3421 cccggcggtt ccatcaagga tcgcccggcc ctgtgcatga ttgaatgcgc ggagcgtgaa
     3481 ggggtcttga ccccggacaa gaccatcatc gaggccacca gcggcaacac cggcatcgga
     3541 ctggccatgg tggcggccgt caaaggctac cgcatcatgc ttgtcatgcc ggagacggcg
     3601 agcctcgagc gccagaaggt cctcaaggcc ctgggcgccg agctcctttt gactcccggt
     3661 tccctgggga cggacggggc catcgaggag gtttacagcc tggtccgaaa ggaccctgat
     3721 cggtatttca tgccggatca attcagcaat cccgccaatc ccctggctca ctacctgggc
     3781 acggggccgg aaatctacga acagacgggc ggaaaggtcg acgccgtggt gatcaccctg
     3841 ggcaccacgg gaaccgccat ggggatcctc aaagcaatga aggatcgaaa tccctccatc
     3901 cgggtgatcg gggtggaacc ctaccccgga cacaaggtcc agggcctcaa gaatatgaaa
     3961 gaatcctatg tccccgccat tttcgaccgt catgccatgg accgaatcct ccccgtgcgg
     4021 gatgaagacg ccttcgagat ggcccgccga ttggcccggg aagaaggcat ctttgcggga
     4081 atgagctcgg gggcggccat ggccgcggcc gtgcaggttg ccgccgagat ggaagaaggc
     4141 accctggtgg cggtccttcc cgacggggga gaccgatatc tgagcacgaa ccttttcacc
     4201 actctgctcg aaccggattt tcatttcttt gatctgctcc gcagggaaaa gacggaattc
     4261 aagccggtac aggaaggcaa ggcccgcctg ttcgtgacca gtccgcccct ggaccagccc
     4321 ctcaccctcc aggaagctcg acgcttcgtc ctggcggacc ttctcacgag gttcctcaaa
     4381 gccaaaggct tttccgtgga tcagatgatc ctcatcccgg atttggaaag ccgcaccatc
     4441 cagggggcca tttccaccgg catggatctc gccgcctact cccagcgcca gatggacgag
     4501 gtgctccgag acctggaccg gttgggagtc cagagggcgc gacggtatcc ccgcatcacc
     4561 gatcacctgg ataccattcc cgagcatacc caggccctca tcgaccggga gctcgcttac
     4621 gaaaagcttc gctcggtcta tttcaacctt tcccggagca gaaattacgg agccctgtcc
     4681 cgggtggacc ccaagaagat tcgagtgggc gccaccgtgg acctggacgc ctacgagaag
     4741 ctgaatcccc gagacttcac actcctcaag cgggccaccc tcacggaact gaaacacggc
     4801 atttacgtca agacctcgtg gggaaacgtg gtgcccacat ggcatatttc cgccgcctcc
     4861 atcgccctgc acgacctggg ggcccccatc gatatcgtgg tgagcagcgt ggacttcctc
     4921 tttccccact tggaaaacgt gcgcgaaatc ggggaggcgc tcaccggcaa gctctttgcc
     4981 aaggtctgga tggtctgcga acggatctgg tccagcaagg aggaaaaggg ctccgatcaa
     5041 gcggacgaga acacccccgt gcgcagcctc acagcttcgg ggacctcccc ggaccagctg
     5101 cgctactggt tcctatgcac ccactaccgg aagcccatcc atttcacccc ggcaaacctt
     5161 aggaacacgc ttcacgggta cgcacgcctc caggaattca tccgccgtgt tcaatacgtc
     5221 tcctgccgaa atgaggagca caagaacatg ggagagttcc tctatgccct cgagcaaggc
     5281 tttttcgacg ctttggcgga cgacctgaac acacccaaag ccctagcagc catgtttcga
     5341 ttcacccggg gggtgaatcc cattctggac agcgggcagt tcagtgaaaa ccagcgcaaa
     5401 cagatcctcg aaacctttca gaagctcaac ggaatcctgg ggattttgga tctggatctc
     5461 gaggccctca gccccgagga ggaagaactg gtgaaggccc gccatgaggc gcgggcctcg
     5521 aaggattggg ccaaggcgga tctccttcgc cgggagctcc ttgatcgcgg catccaggtg
     5581 gtggataccc ctgcgggcac acgttggacg aggatttaat ctccgaagga attcttccgg
     5641 catccccggc ggggagctgt cggcaccgtc ggggacggac ggaacgtcct catcgagtcg
     5701 gcagccgggt cttttccggg gggcgcgggt ttcccccccg agcacaaccc agcaaaaatg
     5761 cgcgtccgtc tccgcccttc aagcagaccc tccttccccc ggacccggga tccggtccgg
     5821 ggcggccccc ggaatccgcg caacgggccc cgttgccggg tttcgccgcg atggaggccg
     5881 tcatgggccc ttcattgggc aaggccgtac tcataagccg gacccgccca ccccccagga
     5941 ctcggacctt tgctcgaaac cttcactggg catgtcgggg cgaccttggg cgcaacttgg
     6001 cattaaaggg ctacaggagc tcgagaacgc cctcggccaa gcctatggag tgggtggcct
     6061 cgaacagtct cagggcttcc tcatatgcgt gttcggtgat ccgtctcttg cattggaggg
     6121 gaatggcgtt catcctggcg gcaaagggct gattcacgtt cttgatgttt ccgccggtat
     6181 tcttggtctc ccgtgcccag gcccacatct cctgccataa ctcgtcgggg attccccgat
     6241 agggtctctt gatgtattca cggttctcgg tggtcagggc gttgccgttc tggttcatgg
     6301 ccaggcccag ggagatgttc tgccacaggg t
//
LOCUS       DAOOVC010000195         6292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7197_length_6292_cov_5.376463, whole genome shotgun sequence.
ACCESSION   DAOOVC010000195 DAOOVC010000000
VERSION     DAOOVC010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6292
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7197_length_6292_cov_5.376463"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLM79_16475"
     CDS             <1..478
                     /locus_tag="PLM79_16475"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOV87953.1"
                     /translation="LGMGSTVVLAWISNLEAIIAHLGDSRAYVLRKGRLHPLTRDHSL
                     VQMLVDFGEIEPGEARTHPARNQITRCVGMPGDPLPEVSIVEIEPRDLLLLCSDGLTG
                     MIEDALIEEILTGCSAADSPLEECARALVDAANNAGGHDNVTVLLIRVDGPHAVDG"
     gene            462..3989
                     /locus_tag="PLM79_16480"
     CDS             462..3989
                     /locus_tag="PLM79_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOV87954.1"
                     /translation="MPLTVECPFCGTRYFKVPDHYLGRSVRCKTCRGKFPAAAASSPG
                     AEPSPPGWRPGSRIGREYEVLETLGEGAFGVVHRVRHRGWNLDLAVKSPRQEVLAAAG
                     GVERFKQEAETWVNLGLHPHIVSCYYVREMQGVPRVFAEYVAGGSLRAWIREKKAADL
                     SRVLDIAVQFAWGLEYAHSRGLVHQDVKPANVMMTVDGTAKVTDFGLARRGTPRAPGP
                     SAPSPDPRATLLGYTPAYASPEQVEGKLVSPKTDLWSFGLCLFELFSGRILWPVGSVA
                     AQVLENFLACPHPFDPLVEMPQSVAELLRRCFQHDPDLRPESMGVVADILREIYREHT
                     GSPYPRIQSRGGTGAADDLNNRALSLLDLGKRQEALDLWNRALEKEAHHPEATFNLGM
                     VLWRSGQKTDEELLQDMEEVKTSHPGHGKIPCLLGLIHMERHDFAAAMKCWDSPEPVG
                     FSFPEFRDAVEECRRRLPNTRKVLRDLEGHTNQVNSVVIGHDGRTALSGGYDRDLRLW
                     DLATGTCLKILQGHGDCVNSVAVDSRGTLALSGSRERQRRLILWDLATGRILRTFEGH
                     KHNVLAVCLSRDGRLAVSGALDGTARLWDTANGTCLQVFPGEKVGVNAVALSRDGRLV
                     LGGGVSGILHLWEASTGRLLRSIAASPGGLIRAVDLAPNAQTAASGDVFGNLCLWDLE
                     TGGCARRFKGHSGEIFGVCFSEDGRSLLSAGGWEKKLRIWEVETGCNRTTLEAENGVL
                     SVALHPGGTLAVTSCRNEIRLWQLHLGGPPFRAPFVLCGARSTEEVLSVRTAFERDLD
                     RARSALARRDWNGALGFVRSARARPGLSRDPAAMDLWLQLYGKLPRKSLKNGWTVRTI
                     RAFQATISFLCLSPDGRLALVLGKDGEALGGTRPDLRLFDVVDGTLLRQLDGEDRGFE
                     EACFAPCGRVAYFARGHTSPQHNRSLGIWNLQTGAVEAEFPTTSQFQSALCAAPDGKR
                     VFLQQGSAVVFYDLAAREFLFYIRDPEGPMEPLYPTPDGRFLLTGTPGGVLRWWDLAT
                     RTPVRTITRQQGTVRRISVSGDGRWAATAGEDQRIRLWNLEDGTPGPVLPGYTGGGPA
                     LSFDGRIVAGSVENRICVWDLETGLLVRTFEGHSEPPDFVRLSRDSATMVSAGRDGTL
                     RRWILDWELEERQTPAEREQPCCTSTTPL"
     gene            4179..>6292
                     /locus_tag="PLM79_16485"
     CDS             4179..>6292
                     /locus_tag="PLM79_16485"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV87955.1"
                     /translation="MQYSPGIEIAMALALNEATVGEFREIRPEHVLMAALKFSEMEGE
                     VLEKSLGNLVPLDLLRDEHRGIRQFLRSRGIDSKPLRRRLRDLLGKGGFPSRGSGLHR
                     SAETRRFFAAAEALAQKEGTEVCSCLHLLCALLDSPTPAVAAVFGPGDSSPAEEEPLP
                     LEEKPPEPARDLGRLTEILKELRGTLLSRVFGQDHAIQTFVEGLFNAEVTAAADTDRR
                     KPKAVFVFAGPPGVGKTYLAECGARTLGKPFRRFDMSGYSDPMGAVLLAGAQRSYQGA
                     GPGQLTEFVRDHPDSILLFDEIEKAHLSVLHLFLQVLDLGSLEDRYLQEPIPFRDTVL
                     IFTTNAGRSLYDDGDTGGAGSGLGIFHRKTVLNALATERDPRTREPFFPPAICSRLAT
                     GYPILFSRLGVCELERIAASELERQGSLLEKQYGKPIQFGPLLPFCLVLREGIPTDAR
                     TVRSQAETFVKSEIFRFCSLNTPEHLRTVWDEARSLRFDLDSAESISEEVRSFLEPRT
                     RFKVLLACRPEPADLWFGGIAEMDRKVAADLADAQNMLAAGDFDLVLLDLWFGQARGS
                     GETAGSQPQTMAQFDHVPVAARGIRRGLELLRILREKVPELPCYLLSSAAGTPFPRID
                     DELLLECVRAGGVRGVIEAPEAFPEKTPGTSPPDLSARIREVAEKLYREKKAAELGSQ
                     NRVLSIRRFRSRMRISSCRERPG"
BASE COUNT         1102 a         2051 c         2000 g         1139 t
ORIGIN      
        1 gctgggcatg ggatcgacgg tggttctggc ctggatttca aacctcgagg cgatcatcgc
       61 tcacctggga gacagtcgtg cctacgtcct gcgcaagggt cggcttcacc ccctcacccg
      121 ggaccattcc ctggtgcaga tgctggtgga cttcggcgag atcgagcccg gggaagcccg
      181 cactcacccg gcgcgaaacc agatcacccg ctgcgtgggg atgccgggag atcccctgcc
      241 cgaggtttcc atcgtcgaga tcgaaccccg ggaccttctc ctcctctgct cggacggcct
      301 gacgggcatg atcgaggacg ccctcatcga ggaaatcctc accgggtgct ctgcggcgga
      361 ctctcccctc gaagaatgcg cccgggcctt agtggatgcg gccaacaacg ccggaggcca
      421 cgacaacgtg acggttctct tgatccgggt ggatggtccc catgccgttg acggttgagt
      481 gtcccttctg cggcacccgg tatttcaagg tacccgacca ctacctgggc cgctccgtgc
      541 gctgcaagac atgccgggga aagttcccgg ccgccgcggc ctcttctccc ggggcggagc
      601 cttccccgcc cggatggagg cccggcagcc gaatcggtcg ggaatacgaa gttctcgaga
      661 ctttgggaga aggggccttc ggcgtggttc atcgggtgcg ccatcgcggc tggaacctgg
      721 accttgccgt gaagagcccc cggcaggaag tgctggccgc tgccggaggc gtggaacgat
      781 tcaagcagga agccgagacc tgggtcaacc tgggcctcca ccctcacatc gtgagctgct
      841 attacgtgcg ggagatgcag ggcgtcccgc gggtgttcgc cgaatatgtt gccgggggaa
      901 gccttcgggc ctggatccgc gaaaaaaagg ccgcggacct ctcccgggtc ctcgatatcg
      961 ccgtccagtt cgcctgggga ctcgagtatg cccacagccg aggcctggtt caccaggacg
     1021 tcaaacccgc caatgtcatg atgaccgtgg acggaacggc caaagtgacg gatttcggcc
     1081 tggctcgaag gggaacgccc cgggccccgg gaccgtccgc cccatccccc gatcctcggg
     1141 ccaccctgct gggatacacc cccgcctacg cctcccccga acaggtggaa ggcaagctcg
     1201 tcagtcccaa gacggacctg tggtccttcg ggctttgcct cttcgagctc ttttcgggaa
     1261 gaatcctctg gcccgttggg tccgtcgccg cccaggtcct cgagaacttc ctggcctgcc
     1321 cccatccgtt cgacccgttg gtcgagatgc cccaatccgt ggcggagctt cttcgacgct
     1381 gttttcagca cgacccggat cttcgccccg aaagcatggg cgtcgtggcg gacattctcc
     1441 gggagatcta ccgggaacac accggatcgc cctacccccg aatccaaagc cggggaggca
     1501 cgggtgccgc cgacgaccta aacaaccggg ccctttcgct cctcgatctt gggaagaggc
     1561 aggaagcgct ggacctctgg aaccgagccc tcgaaaaaga agctcaccac cccgaagcca
     1621 ccttcaacct gggcatggtc ctgtggcgtt cgggacaaaa gaccgacgaa gagcttctcc
     1681 aggacatgga ggaagtgaag acttcccatc ccggccacgg gaagatcccg tgcctcttgg
     1741 ggctcatcca catggaacgt cacgattttg ccgctgcgat gaaatgctgg gacagccccg
     1801 aacccgttgg attctccttc ccggagtttc gggacgccgt cgaagaatgc cgccgaaggc
     1861 tcccgaacac gagaaaggtc ctgcgggacc tcgaggggca caccaaccag gtgaattccg
     1921 tggtcatagg gcacgacggc aggacggccc tgtcgggggg gtacgaccgg gaccttcgcc
     1981 tctgggacct ggctaccgga acctgcctga agattctcca gggacatggg gactgcgtca
     2041 attccgtggc cgtggattca aggggaaccc tggccctgtc cggcagccgc gagcgccaaa
     2101 gacgattgat cctttgggac ctggccacgg gcaggatcct tcgaaccttc gagggtcata
     2161 aacacaacgt tctggcggtg tgtttgagcc gggacggccg gttggccgtc tcgggcgccc
     2221 tcgatgggac ggcccgcctg tgggacaccg ccaacggaac ctgcctgcag gttttccctg
     2281 gagaaaaggt cggcgtgaac gccgtggctc tcagccgtga cggccgcctg gttctcggag
     2341 ggggagtctc gggaatcctg catctgtggg aggcatccac cggccggctc ctccggtcca
     2401 tcgcggcatc ccccgggggc ctgatccgtg ccgtggattt agcccccaac gcccaaacgg
     2461 ccgcctcggg ggacgtcttt ggaaacctct gcctctggga cctggaaacc ggcgggtgtg
     2521 cccgacgatt caaaggacat tccggagaaa tctttggggt ttgtttctct gaagacggga
     2581 ggtccctgct ttccgctggc ggatgggaaa agaaactgcg catctgggaa gtggaaacgg
     2641 gatgcaaccg cacgaccctg gaagcggaaa acggggtcct ctccgtcgct cttcaccccg
     2701 ggggaactct tgccgtcacg tcgtgccgca atgagatccg cctctggcaa ttgcacctgg
     2761 gagggccccc tttccgggct ccgttcgtgt tgtgcggagc ccggagcacc gaggaggtac
     2821 tctcggtgcg gacggccttc gagagagact tggaccgggc gcggtccgcc ctggcgcgcc
     2881 gagactggaa cggggccctg ggttttgtgc ggtcggcccg tgcccggccc ggcctttcga
     2941 gagatccggc cgctatggac ctctggctgc agctctacgg aaaactgccc cggaaatctt
     3001 tgaaaaacgg gtggacggtc cggaccatcc gggccttcca ggcaacgatc tcctttctgt
     3061 gtctgagccc ggacggtcgc ctagccctgg ttctgggcaa agacggggaa gctttggggg
     3121 gtacccggcc cgacttgcgg ctcttcgacg tggtcgacgg gacccttctc cggcaactgg
     3181 acggcgagga tcgcgggttc gaggaggcct gctttgctcc atgcggccgg gtcgcctatt
     3241 ttgctcgggg acacacctcc ccccagcaca atcgcagcct cgggatctgg aatcttcaaa
     3301 caggagcggt ggaagccgaa ttcccaacca cctcccagtt ccaatccgcc ctttgtgccg
     3361 ctcccgacgg aaagcgtgta ttcctccaac agggatcggc ggtcgttttc tacgaccttg
     3421 ccgcccggga gtttctcttc tatatccggg accccgaagg tcccatggaa cccctgtacc
     3481 ctacgcccga cggccgcttt ctcctgacgg gaacccccgg cggggtactg cgatggtggg
     3541 acctggccac ccggacgccg gtccgcacca tcacccggca gcaagggacc gtccggagaa
     3601 tatcggtgag cggcgacggc cgttgggccg ccaccgccgg ggaggaccaa agaatccgat
     3661 tgtggaacct cgaggacggc acccccgggc ctgtcctccc cggctacacg gggggcggcc
     3721 cggccctgag ctttgatggt cgaatcgtcg caggctcggt agagaaccgc atctgcgtgt
     3781 gggacctgga aacagggcta ttggtccgca cctttgaagg ccactccgaa ccccccgatt
     3841 ttgttcggtt gagccgagac agcgcgacga tggtcagtgc agggcgggac ggaaccctgc
     3901 gtcgctggat cttggattgg gaactcgagg agcgccaaac acccgccgag agggaacagc
     3961 cttgctgcac cagcaccacg ccgctctgag ggagccgggt tcgagaccgt ctcaagaccg
     4021 ctttcgcacc gctcatggtc gttccctccg ggggctttcc gggcagtgat tcccgggttg
     4081 gaggccgggg cggcggcggc cgtttcatcc ccgggacgcc ccacggagtg catactccgg
     4141 gtcgacatgc gcatcggaac tgaagaaagg agattgccgt gcaatactct ccgggcattg
     4201 agatcgccat ggccctggcc ctgaacgaag ccaccgtggg agagtttcgc gagatccggc
     4261 cggaacacgt cctcatggcc gccctcaagt ttagtgaaat ggaaggggag gtcctggaaa
     4321 agtccctggg gaacctggtg cccctggatc ttctccggga cgagcaccgg ggaattcggc
     4381 agtttctccg atctcggggg atcgactcga agccgctgcg cagaaggctt cgagatctgc
     4441 tgggcaaagg aggttttcct tcccggggct cgggcctgca ccggtcggcg gaaacccggc
     4501 gcttttttgc cgccgccgaa gccctggccc aaaaggaggg aaccgaagtc tgctcgtgcc
     4561 tccacctgct gtgtgccctc ctggactccc ccactccggc cgttgccgcc gtcttcggcc
     4621 ccggggattc ttcccccgcg gaggaggagc ccctccccct cgaggagaag ccccccgagc
     4681 ctgcccggga tctcgggagg ctcacggaga ttttgaaaga gcttcgtggg acccttcttt
     4741 cccgggtctt cggccaggac cacgcaatcc agaccttcgt ggaaggcctc ttcaatgccg
     4801 aagtgaccgc cgcggcggac acggatcgaa gaaagcccaa ggccgtgttc gtctttgccg
     4861 ggcccccggg agtgggcaag acctatcttg ccgaatgcgg ggcccggacc ctgggaaaac
     4921 ccttccgccg cttcgacatg agcgggtatt cggaccccat gggcgcggtg ctcctggcgg
     4981 gcgcccagcg aagctatcag ggagcgggcc ccggacagct cacggaattc gtgcgggatc
     5041 atcccgattc gatcctcctc ttcgatgaaa tcgagaaggc ccacctttcc gtcctgcacc
     5101 ttttcctcca ggtcctggat ttggggagcc tcgaggacag gtacctccag gaaccaatcc
     5161 cttttcggga caccgttctc atcttcacca ccaacgcggg ccggtctctt tacgacgacg
     5221 gggacacggg aggagccggg tccggcctcg gcatttttca tcggaagacc gttttgaacg
     5281 ccctcgcgac ggaacgggac ccgcgaaccc gggaaccctt ctttcccccg gccatctgtt
     5341 cccgcctggc tacgggatac ccgattcttt tcagccggtt gggggtatgc gagctcgaac
     5401 ggattgccgc ctcggaactc gaacgacagg ggtctttgct cgagaagcaa tatggaaagc
     5461 ccatccagtt cggaccgctc cttcccttct gcctggtgct gcgggaagga atccccaccg
     5521 atgcccggac cgttcgatcc caagcggaga ctttcgtcaa gtccgaaatc ttccgctttt
     5581 gttccctcaa caccccggaa cacctgagga ccgtctggga cgaggcgagg agccttcggt
     5641 tcgacctgga ctccgccgaa tccatcagcg aagaggttcg gtcgtttctc gaaccccgga
     5701 cccggttcaa ggttctgctg gcgtgccgtc cggaaccggc ggacctgtgg ttcgggggta
     5761 ttgccgagat ggaccggaag gtggccgccg atctcgcaga cgcacaaaac atgctggcgg
     5821 cgggggattt cgacctggtc cttctcgacc tgtggttcgg acaggcccgg ggctcggggg
     5881 aaaccgccgg gtcccagccg caaaccatgg cccagttcga ccatgtgccc gtggccgccc
     5941 ggggaatacg ccggggcctg gagctgctgc gcatcctccg ggagaaagtt cccgagcttc
     6001 cctgttacct cctctcctca gcggccggga ctccgtttcc ccggatcgac gacgagcttt
     6061 tactcgagtg cgttcgagcg ggaggggtgc ggggcgtcat cgaggccccc gaggcgttcc
     6121 cggaaaagac cccgggcacg tctcccccgg acctctccgc caggatacgg gaggtcgccg
     6181 aaaagcttta ccgggaaaag aaggccgcgg aactgggatc ccaaaaccgg gtgctatcca
     6241 ttcgaaggtt ccgaagccgg atgaggatct cctcctgccg ggagaggccc gg
//
LOCUS       DAOOVC010000196         6256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7244_length_6256_cov_6.716175, whole genome shotgun sequence.
ACCESSION   DAOOVC010000196 DAOOVC010000000
VERSION     DAOOVC010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7244_length_6256_cov_6.716175"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLM79_16490"
     CDS             complement(<1..726)
                     /locus_tag="PLM79_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV87956.1"
                     /translation="MFRRCLVPLDGSRLAESVLPAVRRFGFPGDCLVTLLHVVEKGAP
                     ASVHGDVHLQEVRQAQTYLESVAAKLRADGLKVELHVHEVPQEDVPRGIADHAKELDK
                     DLIVLCSHGRGGWRRFVFGSNAERVLAHGTTPVLLIRPNENGVAPPVGPHRIVILLDH
                     LPETVLALEAGGELAERSGAELLLLGVVPTVERASGDLSPTSRLAPRTARYLRDMEAE
                     DLAERLAGEVEKLTARRVRAGARV"
     gene            complement(796..2097)
                     /locus_tag="PLM79_16495"
     CDS             complement(796..2097)
                     /locus_tag="PLM79_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOV87957.1"
                     /translation="MGEQDLPSPPPSGSAGTVLAAEAVLDHTGALPWYRRFLPFLGPA
                     FVAAVAYIDPGNFATNIQGGALFGYTLLWVIGASNLMAMLIQVLSAKLGIATGQNLAE
                     VCRDRFPAPLVWIMWGIMELVAMATDLAEFLGAALGFYLLFHIPLLWAGLLTAVITFV
                     VLGLERFGFRPLEAVISALLGVVALCYLVEILLCELQWKEIARHALVPGFEGKESVFL
                     AAGILGATVMPHVIFLHSALTQGRVVVRDPGKLRRLFRFQLMDVVMAMGVAGLVNGAM
                     LIMAASSFHQQGLHRVGTIEEAYRTLTPLLGPMAAGVFAVSLLASGISSSTVGTLSGQ
                     VIMQGFMRFHIPVWIRRGVTLLPSLAVIAGGLDPTRTLVISQVILSFGLPFAVIPLVL
                     FTSNPRIMGVLVNAKVTTLAASVVAGLIVSLNLYLLWTLVS"
     gene            complement(2280..3365)
                     /gene="serC"
                     /locus_tag="PLM79_16500"
     CDS             complement(2280..3365)
                     /gene="serC"
                     /locus_tag="PLM79_16500"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOV87958.1"
                     /translation="MTQRIANFNPGPAALPLPVLEEIQQEFLDFRSSGMSILEISHRS
                     KLFESVLNDAAERTKRLLKLDHRYQVLFLQGGASLQFCMVPMNLAIPGKPSAYVDTGT
                     WASNAIKEARVLEKDVHVVASSQDREYTYIPSSIPVPPGASYLHITSNNTIRGTQWDR
                     FPDAGDTPLVSDMSSDIFSRAFDPAPFGFIYAGAQKNAGPAGVTLVVIRNDLLERVPA
                     NLPTMLKYTTFAGKNSMYNTPPCFAVYVVRLVLKWIEETVGGLEKMENLNRRKAKLLY
                     DYLDSQDFYRGTAEPGSRSLMNVTFRLPSEELEQKFIRESLAESLGGLKGHRSVGGCR
                     ASLYNAVGMEWVESLVSFMKRFVEKNG"
     gene            3604..4455
                     /locus_tag="PLM79_16505"
     CDS             3604..4455
                     /locus_tag="PLM79_16505"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HOV87959.1"
                     /translation="MNIQELAEPRNDLSGGPPWGIDDDSLLEYARRTGSTPGQAQREA
                     LDNGILPLRYEKNLASLDFREQVLLSKSRVLICGCGGLGGVLIHLLARAGVGFMRLVD
                     GDVFAVSNFNRQWFCETGQIGRPKAPATRSRLSAINPFVEVEAFQAHLDEGNGSGFLA
                     GMDLALDALDNLPGRFILGEAACRAGIPYIHAAVAGWWGQISTFPPGFPGDLGLIYGR
                     TRDRNAREESLGVLGPAVSCIASLQSMEALRLLAGRSPAYAGKLLYLDGETGHVEIVP
                     LRGPTAR"
     gene            4472..5989
                     /locus_tag="PLM79_16510"
     CDS             4472..5989
                     /locus_tag="PLM79_16510"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOV87960.1"
                     /translation="MRIEWRISAPPHWQAEALLFFVPEKTPQPPEGLVQWIRQGAQWL
                     AQSPALRDFQGKIQQVAVFYDPGDGPVPRVLCCGLGPPEDFHPERLRMAAAAGLRKCR
                     ELRLKEAALPLSAFAGLPCEAAVALEEAVLGAVTGLHQYDALKTRDVEPTSFPETLCL
                     LAEREPEPAVRQAPFLAEAVSSGILLARDLTVAPANEITPSYLAGTARRLAEAYGFTL
                     DVIDLETAREMGMGAFAAVAQGSREPPAFIVLEHKSLETGFKKPLVVVGKGITFDTGG
                     ISLKPGDKLDSMKQDMAGAAAVLGLFEVLGKTRWERHVVGILPCTENMPGGRAYKPGD
                     VLRTLAGLTVEVISTDAEGRLVLCDALAYARRYSPALILDIATLTGACIVALGKQVAG
                     IMGNRESLVQRIQQVGMDIGERLWPLPLWDLYFDSIKSDVADIKNLSDRAAAAIIGGV
                     FLNQFVPREIPWIHLDIAGTAWSDKETGAFPKGPTGFGVRLFFELLRRWEELGLE"
BASE COUNT         1205 a         1983 c         1953 g         1115 t
ORIGIN      
        1 cacccgggct cctgctcgaa ctcggcgtgc ggtcagcttc tccacttccc cggccaggcg
       61 ttccgccagg tcctccgctt ccatgtccct caggtaacgg gcggttcggg gggccaggcg
      121 gctggttgga gaaagatccc cggacgccct ttccaccgtc ggcaccacgc ccaggagcag
      181 cagctcggct ccgctgcgct cggccagttc cccgccggcc tcgagggcaa gaaccgtttc
      241 ggggagatga tccaagagga tcacgatccg atggggaccc acgggaggag cgactccgtt
      301 ctcgttgggc cggatgagca ggacgggagt ggtcccgtga gcgaggaccc gctctgcgtt
      361 gcttccgaaa acaaaccggc gccatcctcc tcttccgtga ctgcaaagaa cgatcaggtc
      421 cttgtccagt tccttggcgt ggtcggcaat tccccgggga acatcctctt gaggcacctc
      481 gtgcacgtgg agttcaacct tgagaccgtc ggctcggagc ttcgccgcaa cggattccag
      541 gtaagtctgc gcctggcgga cttcctggag gtggacgtcc ccgtggacgg aagccggagc
      601 ccccttctcc accacgtgga gcagcgtgac cagacaatct cccgggaatc cgaacctgcg
      661 tacggcgggc aacacggact cggccaggcg ggaaccgtcc aagggaacca ggcatcgacg
      721 aaacatgggg aaattccgtc tcctctccac gagcctcccg gcaggggcgg cccctcaggc
      781 accgaccccg tcgggtcagc tcacgagggt ccaaaggagg tacaggttca gggagacaat
      841 gagccccgcc accacggaag ccgcgagggt cgtgaccttt gcgttgacca ggacgcccat
      901 gattcgaggg ttgctggtga acagcaccag cggaatgacc gcaaagggca acccaaagct
      961 gagaatgacc tggctgatca ccagggttcg agtggggtcc aaccccccgg caatcaccgc
     1021 cagggacggc aacaaggtca ccccacggcg aatccatacg ggaatgtgaa accgcatgaa
     1081 accctgcatg atcacctgcc cggacagggt ccccaccgtc gaggaggaaa tccccgaagc
     1141 cagcagggaa acggcaaaca ccccggcggc catgggtccc agcagcggag tgagggtgcg
     1201 gtacgcttcc tcgatggtcc ccacccggtg cagcccctgc tgatggaagg aggaagcggc
     1261 catgatgagc atggccccgt tcaccagtcc cgccacgccc atggccatga ccacgtccat
     1321 gagctgaaag cggaagagcc ttcgaagctt tccgggatcc ctcacgacca ctcgtccctg
     1381 ggtcagggcc gagtggagaa agatcacgtg gggcatgacc gtggccccca gaatgcccgc
     1441 cgccaggaac acgctctcct ttccctcgaa gcccggcacc agggcatggc gggcaatttc
     1501 cttccattgg agctcgcaca agaggatttc caccaggtaa caaagagcga ccacccccag
     1561 cagcgcactg atcaccgcct cgaggggccg aaagccgaac cgttcgagtc ccaggaccac
     1621 gaaggtgatc acggcagtga gcagcccggc ccagaggaga ggaatatgga aaagcagata
     1681 gaaccccaag gcggccccca ggaattccgc caggtccgtt gccatggcca ccagctccat
     1741 gatgccccac atgatccata ccaggggggc gggaaagcga tcccggcaga cctccgccag
     1801 gttttgaccc gtggcgatcc caagctttgc ggacaaaacc tggatcagca tcgccatcag
     1861 gttgctcgcc ccgatcaccc agaggagagt atatccgaag agggctcccc cttgaatgtt
     1921 ggtggcgaaa ttacccgggt caatgtaggc gaccgccgcc acaaaagcag gccccaggaa
     1981 aggcaggaaa cgccggtacc agggcaatgc cccggtgtgg tccaacacgg cctcggccgc
     2041 caggacggta ccggcgctgc cgctgggagg gggcgatggg aggtcctgtt cacccacgac
     2101 gatcggatcg atttcgattg gcaggttcct gggtcacgac gagaggtctt ctcgtcccgg
     2161 acgggtgccc gggaaaaccc tcgggcgggg gaaggcctcg gtgtcggggt cctccccccc
     2221 gggagtgtac gaagagaatt tgccgctgaa aaatgcccga agagacaaca accggggatc
     2281 tacccgttct tttccacaaa tcgtttcatg aaactcacca gggattccac ccattccatc
     2341 ccgacggcat tgtacaggga agccctgcat cctcccacgg aacggtggcc cttgagccct
     2401 cccaaggatt cggccaggga ttcccggatg aatttctgtt cgagctcttc actgggaagc
     2461 cggaaggtca cgttcatcaa cgacctcgag ccgggctcgg cggtgcctcg gtagaaatcc
     2521 tggctgtcca ggtaatcgta aagcagcttt gccttccgcc ggttcaggtt ttccattttc
     2581 tccagtccgc ccacggtctc ctcgatccat ttcaggacca gccggaccac gtaaacggcg
     2641 aagcagggag gcgtgttgta catggaattc tttccggcaa aggtcgtgta cttgagcatg
     2701 gtcggcagat ttgcgggcac gcgctcgagc aggtcgttgc ggatcaccac cagggtgact
     2761 cccgcgggac cggcattctt ctgggccccg gcatagatga agccgaaggg ggcggggtcg
     2821 aacgcccggc tgaagatgtc cgaagacatg tcgctcacca ggggcgtatc cccggcatcg
     2881 gggaatcgat cccactgggt tccccgaatg gtgttgttgg acgtgatgtg caggtacgac
     2941 gcgccggggg gcaccgggat cgaggaaggg atgtaggtgt attcccgatc ctgggagctc
     3001 gccaccacgt gaacgtcctt ttccaggacc ctcgcctcct tgatggcgtt gctcgcccag
     3061 gtgcccgtat ccacgtaggc cgagggtttg ccgggaattg ccaggttcat gggaaccatg
     3121 cagaattgca gactcgctcc cccctgaagg aacagcactt ggtagcgatg gtccagtttc
     3181 agcagtctct tggtgcgttc ggccgcgtcg ttcagcaccg actcgaacag cttggaccgg
     3241 tggctaatct ccaatatgga cattcccgac gagcggaaat ccaggaactc ctgttgaatt
     3301 tcctccaaaa ccgggagagg caacgcggcg ggccccgggt tgaagttggc gatgcgctgg
     3361 gtcatactct tcctcctctg gcctcacggg gtataaggac cgcttcatcg tcaagacccg
     3421 gccaagctac tgaggccccg gcggtttgtc aacgagaatt tcccccggga aaccggccga
     3481 cggacgtcgg gcaggaagtc ccgagcctca cggaattgct gaaagaacga ggcggatggg
     3541 ctatgatgaa aaacgggcaa cgaggggtga attcttcggg aaccgagcag gacgaaacgg
     3601 accatgaaca ttcaagaatt ggccgaaccg cggaacgatc tttcgggagg gccgccgtgg
     3661 gggatcgacg acgattcgct cctcgagtat gcccggcgca ccgggtcgac gccggggcag
     3721 gcccagagag aagccctcga caacgggatt cttcccctcc ggtatgaaaa gaaccttgct
     3781 tccctggatt tccgggaaca ggtgcttctc tccaaaagtc gtgtcctaat ttgcggctgc
     3841 gggggcttgg gaggcgtgtt gatccacctt ctggcccggg ccggggtcgg gttcatgcgc
     3901 ctggtggacg gcgatgtttt tgccgtctcc aatttcaatc gtcaatggtt ctgtgaaacc
     3961 gggcagatcg ggcgacccaa ggccccggcg acccgaagcc gtctgtcggc aatcaacccg
     4021 tttgtggagg tggaagcgtt ccaggcgcac ctggacgaag gcaacggatc ggggttcctg
     4081 gcggggatgg atctggccct ggacgccctg gacaacctcc ccggccggtt tatcctgggg
     4141 gaggcggctt gccgggcggg aattccttac atccacgcgg ccgtggccgg gtggtggggt
     4201 caaatctcca cgtttccgcc gggtttcccg ggagacctgg gactcatcta cggtcgaacc
     4261 agggaccgga atgcccggga ggaatccctg ggagtcctgg gacccgcggt ttcctgcatt
     4321 gcaagcctcc agtccatgga ggccctgcgt ctcctggcgg gcagaagccc ggcttatgca
     4381 ggcaaactcc tgtacctgga cggagagacc ggccacgtgg agatcgtccc cctacggggc
     4441 cccacggctc gctgattcga gaggagaaac catgcgtatc gagtggcgga tttcggcccc
     4501 cccccattgg caggcggaag ctcttctttt ctttgtcccg gagaagaccc cccagcctcc
     4561 ggaagggctg gttcaatgga tccggcaggg cgcccagtgg cttgcccaat ccccggccct
     4621 ccgggacttt caaggaaaaa tccaacaggt tgccgtcttc tacgatcccg gggacgggcc
     4681 cgttccccgg gttctctgct gcggcctggg cccccccgag gatttccacc cggagagact
     4741 gcgtatggcc gccgccgcag ggcttcgcaa gtgccgggaa ctgcgtctca aggaagcggc
     4801 ccttcctctc tccgccttcg ccgggcttcc ctgcgaggcc gccgttgctc tcgaggaagc
     4861 cgtcctcggt gccgttaccg gccttcatca atacgacgcc ctcaagaccc gggacgtgga
     4921 gcctacctcg tttcccgaaa ccctttgtct cctggcggaa cgggaacccg aaccggccgt
     4981 ccggcaggca cccttcttgg ccgaagccgt ctcttccggg atcctcctgg cccgggactt
     5041 gaccgtggcc ccggccaacg agatcacccc gtcctacctt gccgggacgg cccggcgact
     5101 ggcggaggct tacggcttca ccctcgacgt catcgacctc gaaacggccc gggaaatggg
     5161 catgggagcc tttgccgccg tggcccaagg cagcagggaa ccccccgcct tcatcgtcct
     5221 ggagcacaag agcctcgaaa ccggcttcaa aaagcccctg gttgtcgtgg gaaagggaat
     5281 caccttcgac acgggaggca tttccctcaa acccggcgac aagctcgact ccatgaaaca
     5341 ggacatggcg ggagccgccg ccgtgctggg tctgtttgag gtcctgggta aaacacgatg
     5401 ggaacggcac gtggtgggaa tcctcccctg cacggagaac atgccggggg ggcgggccta
     5461 caaaccgggg gacgtgctgc ggaccctggc gggcttgacc gtggaggtga tcagcacgga
     5521 cgccgaaggg cgcctcgtcc tgtgcgacgc cctggcctat gcccggcgat attcccccgc
     5581 cttgatcctc gatatcgcaa ccctcaccgg agcctgcatc gtcgccctgg gcaaacaggt
     5641 cgcaggaatc atggggaatc gggaatccct ggttcaacga attcagcaag tggggatgga
     5701 catcggcgag aggctctggc ccctgcccct ctgggacctt tacttcgaca gcatcaagag
     5761 cgacgtggcc gacatcaaga acttgagcga ccgagctgct gcggccatca tcggcggtgt
     5821 ctttctcaac cagttcgtcc cccgggaaat cccctggata cacctggaca tcgcagggac
     5881 cgcctggtcg gacaaggaaa cgggagcgtt ccccaaggga cccacggggt ttggggtccg
     5941 attgttcttt gagctgcttc gacggtggga agaactgggt ctcgaatagg gcgacgggcc
     6001 ggggaaaccc cggcagaccg gagtttcccc aattcccggt tcaaatcctt atggggaggg
     6061 aggaccggaa ctgtgggtcg gggaatcgat ctcctgagcc tcccggcctc ccagcccctt
     6121 actggacttc ctgtcctgga cgaggttttt gcttcttttc tccggcctgg cccggggttt
     6181 tgttttcccg ttgggcactc ggttcccggg gttctctcga ctcacgcatc tgctgccgtt
     6241 cttcccggcg ctgctg
//
LOCUS       DAOOVC010000197         6156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7389_length_6156_cov_6.929356, whole genome shotgun sequence.
ACCESSION   DAOOVC010000197 DAOOVC010000000
VERSION     DAOOVC010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7389_length_6156_cov_6.929356"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1903
                     /gene="ftsH"
                     /locus_tag="PLM79_16515"
     CDS             2..1903
                     /gene="ftsH"
                     /locus_tag="PLM79_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOV87961.1"
                     /translation="MDRFMKHLKGTSGEGDPRGQDPSQRRFHFSFWYFLVALLLVAWV
                     QTYLVEPQRERVTYSEFKQWVRDGRVGSLILGPERVRGELKQEGGSGIQFTTVRVEDP
                     ELVPLLDSKGIQYSGYIENKWIAVLLSWLLPMAVFILLWSFLMRKMSGGPQGVLSVGK
                     ARVKIFAEQEMEITFDDVAGIDEAKTELQEIVEFLKTPEKYRRLGGKIPKGVLLVGAP
                     GTGKTLLAKAVAGEAGVPFFSMSGSEFVEMFVGVGAARVRDLFAQAKTNAPCIIFVDE
                     LDALGKARGLNPVGGHDEREQTLNQLLVEMDGFDPRAGVIIMAATNRPEILDPALLRP
                     GRFDRHVAVDKPDIRGREAILKIHARDVRLAPEVDLKKIAAMTPGFVGADLANLVNES
                     ALIAARRDRENVTMADFHEAADRIIGGLEKKNRAMNPKEKEIVAYHESGHALVAMSVP
                     RADPVSKVSIIPRGIAALGYTQQLPTEDRYLMTRNELMDRLQVLLGGRVAEEMIFGDI
                     STGAQNDLQRATDIARSMVTEYGMSERLGPLTYTRDPRSAHLDLGFRTREREYSEQTA
                     QSIDEEVTRIIEEAHQKSRDILNEHREHLQRLAKVLLEKESVEGEELREFAREVKEGL
                     ARHTEAATH"
     gene            complement(1860..2546)
                     /gene="nfi"
                     /locus_tag="PLM79_16520"
     CDS             complement(1860..2546)
                     /gene="nfi"
                     /locus_tag="PLM79_16520"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697132.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HOV87962.1"
                     /translation="MNHEPTWDLTPQEAKRLQEDLASQVCLRPLPTDFGLLGAADIGY
                     VPSIRRLVAVMMTFLWPGLELVESSHVVCDISFPYIPGLLSFREIPPLLAAHSKLSHP
                     PDVLLCDGHGIAHPRRLGLASHLGLCLRIPTVGVAKKRLCGTHGPLNLRRGNFTPLYL
                     NGDVVGYVYCSRDNVKPLYLSPGHLADLNSSTHLVERCLGKYRIPEPLRQTHRVASEL
                     RLQYGGPDLP"
     gene            complement(2657..4843)
                     /locus_tag="PLM79_16525"
     CDS             complement(2657..4843)
                     /locus_tag="PLM79_16525"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOV87963.1"
                     /translation="MTVLRPESTLQLTPNALTVLKKRYFAKNEKGEPIETPEELFWRV
                     AQAIAQGDAAYDGQAKVEETAYRFYRLMTSLDFLPNSPTLMNAGRSLGQLSACFVLPV
                     EDSIESIFEAIKHAAMIHKSGGGTGFSFSRIRPERDKVLSTHGVASGPVSFMTVFDTA
                     TETIKQGGTRRGANMGILRVDHPDIEHFITCKTDNERLTNFNISVAVTDLFMEAVEKD
                     ADYDLVNPRDGRTVKTLSARRVFDQIVQSAWKNGEPGIIFLDTVNRHNPTPRVGQIES
                     TNPCGEQPLLPYESCNLGSINLANMGQDGSIDYDRLKAVVWDAVHFLDNVIDVNRYPL
                     PKIRELTFSNRKIGLGVMGFADLLIELGIPYNSEEAVSTAERIMEFIQRESKAASAAL
                     AEQRGNFPNYPESLYDKPETPFMRNATTTTIAPTGTISIISGCSSGIEPIFAISFVRK
                     VLDNQELIEVHPAFQAVARRRGFYSEELMKRIAERGSIQDFKEIPEDVRRRFVISHDV
                     TPEWHIRIQAAFQKFTDNAVSKTINFPFTATVEDVQNAYISAYKLGCKGLTIYRDGSR
                     DVQVLNIQRKPKYPQVEPRPRPDKTLGVTERINTGCGKLYVTINRDEYGFCEVFAQMG
                     KAGGCAYSQIEATGRLISLALRSGVKVESVIRQIMGIRCPSPIWQNGEQVVSCSDAIA
                     KVLNHQVQTTVEQSSDLMGACPDCGAFVEHEGGCIVCRACGFSRCS"
     gene            5434..5856
                     /locus_tag="PLM79_16530"
     CDS             5434..5856
                     /locus_tag="PLM79_16530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOV87964.1"
                     /translation="MRLSTRSRYGMRLLLDMAWHYGRGPIQLAVIAKRQGIPLKYLEQ
                     IVIPLKKAHYVQSVRGPRGGHMLAKSPSEINVGEIVFLLERKAQLVECVNNPEVCERS
                     STCVTRTLWKETTEAICEKLNSISLSDIMERSPTELDL"
BASE COUNT         1279 a         1718 c         1916 g         1243 t
ORIGIN      
        1 catggataga ttcatgaaac atctgaaagg aacctccggg gaaggagatc cccgtgggca
       61 ggatccgtcc cagaggaggt tccatttctc gttttggtat ttcctcgtgg ccctcctcct
      121 ggttgcctgg gtccagacct acttggtgga acctcagagg gagcgggtaa cctactcgga
      181 gttcaaacaa tgggttaggg atgggcgggt cgggagcttg atcctgggtc ccgagcgtgt
      241 gcggggggag ctcaaacagg aaggcggttc cggaatccag ttcaccaccg tgcgggtgga
      301 agaccccgaa ctggttcccc tcctcgacag caaggggatt cagtacagcg gctacattga
      361 aaacaagtgg attgccgttc tcctgtcctg gctcctgccc atggccgtct tcatcctcct
      421 gtggagcttt ctcatgcgaa agatgagcgg cggcccccag ggagtgcttt ccgtgggaaa
      481 ggctcgagtg aagatttttg ccgagcagga gatggaaatc accttcgacg atgtggcggg
      541 cattgacgag gccaagaccg agctccagga aattgtcgag ttcctgaaga cccccgaaaa
      601 gtaccggcgc ttgggaggca agattcccaa aggcgtcttg ctggtggggg ctcccggaac
      661 gggaaagact ctcctggcca aagccgtggc gggggaagca ggggttcctt ttttcagcat
      721 gagcggctcg gagttcgtgg aaatgtttgt gggggtggga gccgccaggg tgcgggacct
      781 gtttgcccag gcaaagacca atgccccctg catcatcttc gtagacgaac tggatgccct
      841 gggaaaggcc cgggggctga atccggttgg aggacacgac gagcgggaac agaccctgaa
      901 tcaactcctg gtggaaatgg acgggttcga tcctcgcgcg ggagtgatca tcatggcggc
      961 caccaaccgc ccggagatcc tggacccggc cctccttcgc cccgggagat tcgaccggca
     1021 cgtggccgtc gacaagccgg atattcgggg ccgggaagcc attctgaaga ttcatgcccg
     1081 ggacgttcgg ctggctccgg aagtggacct gaaaaagatt gccgccatga ctccgggctt
     1141 tgtgggggcc gacctggcca acctggtgaa cgaatccgcc ctcatcgccg cccgtcgcga
     1201 ccgcgagaac gtcaccatgg cggattttca cgaggcggcg gacaggatca tcgggggcct
     1261 tgaaaagaag aaccgggcca tgaatcccaa ggaaaaggaa atcgtggcct accacgaatc
     1321 cggccatgcc cttgtggcca tgagcgtgcc ccgggcggac ccggtgagca aagtctccat
     1381 cattccccgg gggatcgccg ccctgggcta cactcagcag ctccccaccg aggaccgcta
     1441 cctcatgacc cgcaacgagc tcatggatcg cctccaggta ctcctgggtg gacgtgtggc
     1501 ggaggagatg atcttcgggg atatctcgac gggcgcccag aacgacctgc agcgcgcaac
     1561 ggacattgcc cggagcatgg tcaccgagta tgggatgagt gagcgtctgg gaccgctcac
     1621 ttacacccgg gaccctcgtt cggctcacct ggacctggga tttaggaccc gggaacggga
     1681 atacagcgag cagacggccc agagcatcga cgaggaggtg actcgaatca tcgaggaggc
     1741 tcatcagaag tcccgggaca ttctaaacga gcaccgggaa cacctccaac ggttggccaa
     1801 ggttttgctg gaaaaggaat ccgtggaagg ggaggaattg agggaattcg cccgggaggt
     1861 caaggaaggt ctggcccgcc atactgaagc cgcaactcac tagccaccct gtgagtctgt
     1921 ctcaggggtt cggggatgcg atacttcccc aggcaacgct ccaccaggtg ggtcgagctg
     1981 ttcaaatccg caagatgacc cggcgaaagg tataggggct ttacgttgtc ccgggaacaa
     2041 tacacatacc ccaccacgtc tccgttcagg taaaggggcg tgaaattccc ccggcgcaga
     2101 ttgagaggac cgtgggtgcc gcacagcctc ttttttgcaa cccccaccgt agggatcctc
     2161 agacacaatc ccagatgaga agccaggccc agacgacgcg gatgggcgat tccatgaccg
     2221 tcacaaagca gcacgtcggg gggatgactc agcttgctgt gggcggccag gagcggtgga
     2281 atttcccgga aggaaagaag acccgggatg taaggaaagg aaatgtcgca aaccacatgg
     2341 ctgctctcca caagctcgag acccggccac aggaacgtca tcatgacggc gaccagccga
     2401 cggatcgaag gcacataccc gatgtccgcc gctcccagca ggccaaaatc cgtggggagc
     2461 ggccggagac aaacctggga ggcgaggtct tcctgcaggc gcttggcttc ctggggggtc
     2521 aagtcccagg tcggttcgtg gttcattgcg ctcatccggc tgtgcccaga agaatgatcc
     2581 cgtgtttcca cagcgcccca tcggggtgta gtgtgaaaat ccctccccca cggccgccgg
     2641 aggggtcgcc cgcgcgctaa ctgcaacgcg agaaaccgca cgctcggcaa acgatgcaac
     2701 cgccctcgtg ttccacgaac gctccgcagt cggggcaggc ccccatcagg tccgagctct
     2761 gctcgacggt ggtttgtacc tggtggttga gcacctttgc aatggcgtcg gaacaggaga
     2821 ccacctgttc cccgttttgc cagatggggg aagggcaccg tatccccatg atctgccgga
     2881 tgacggactc gaccttgacc cccgagcgaa gggccaggga gatgagacgc cccgtggcct
     2941 cgatctggga ataggcgcat cctcccgctt ttcccatctg ggcgaacact tcgcaaaagc
     3001 cgtactcgtc ccggttgatg gtgacgtaaa gctttccgca ccccgtattg atgcgctcgg
     3061 tgacccccag ggtcttgtcc ggtcgaggcc ggggctccac ctggggatac ttgggcttgc
     3121 gctggatgtt gagcacctgc acgtcccgac tgccgtcccg atagatggtc aaacccttgc
     3181 aacccagctt gtaggcgctg atgtaggcat tctgcacgtc ttccaccgta gcggtgaagg
     3241 ggaaattgat ggtcttgctc acggcattgt ccgtgaattt ctgaaaggct gcctgaatgc
     3301 ggatatgcca ttcgggagtc acgtcgtggg agatgacgaa cctgcgacgc acatcctcgg
     3361 ggatctcctt gaaatcctgg atgcttcccc gttcggcaat ccgcttcatg agctcttccg
     3421 agtagaaccc ccttcgccgg gccaccgcct ggaaggcggg atgcacctcg ataagttcct
     3481 gattgtccag gaccttccga acgaaactga tggcaaagat gggctcgatt ccgctggaac
     3541 accccgagat gatgctaatg gtgcccgtgg gagcgatggt ggtggtggtg gcgtttcgca
     3601 tgaacggagt ttcgggcttg tcgtaaaggg actccgggta gttgggaaaa ttcccccttt
     3661 gctcggccag ggcggcggat gccgctttgg attccctttg aatgaattcc atgatcctct
     3721 cagctgtgga aacggcttcc tccgagttgt agggaatccc cagctcgatg agaagatcgg
     3781 caaagcccat gacacccagg ccgattttcc ggttggaaaa ggtcagttcc cggatcttgg
     3841 ggaggggata ccggttcaca tcgatcacgt tgtccaagaa gtgaacggcg tcccagacaa
     3901 cggccttgag acggtcgtag tcgatggagc cgtcttgacc catgttggcc aggttgatgg
     3961 atcccaggtt gcaggattcg taagggagaa gaggctgttc tccgcaggga ttggtgctct
     4021 cgatttggcc cacccggggg gttgggttgt gccggttgac ggtgtccagg aagatgattc
     4081 ccggctcccc gtttttccag gccgattgaa caatctggtc gaaaaccctc ctcgctgaaa
     4141 gagtcttgac cgtccggccg tccctgggat tgaccaggtc gtagtccgca tccttttcca
     4201 cggcttccat gaagaggtcg gtcacggcca cggaaatgtt gaagttggtc aatcgttcgt
     4261 tgtcggtctt acacgtgatg aaatgctcga tatccggatg gtccacccgg aggatcccca
     4321 tgttggcccc tctgcgggtt cccccctgct tgatggtttc cgttgccgta tcaaaaaccg
     4381 tcatgaaaga taccggcccg ctggcaaccc cgtgggtgga aagcacctta tcccgttcgg
     4441 gacggatccg ggagaaggaa aatccagtgc ctccgccgct cttatggatc atggccgcgt
     4501 gcttgatggc ttcgaaaatg ctctcgatgg agtcctccac cgggagaacg aaacaggcgg
     4561 acagctgtcc caggcttcgc ccggcattca tcagggtcgg ggagttgggc aggaagtcca
     4621 ggctggtcat gagtcggtag aaccggtagg cggtttcctc cacctttgcc tgcccgtcgt
     4681 aggcggcgtc cccttgggcg atggcctggg ccacacgcca gaacaactct tccggcgttt
     4741 cgatgggttc acccttttcg ttcttggcga aatagcgttt cttcagaacg gtcaaggcat
     4801 tgggagtcag ctgcagggtg ctctcgggtc tcagtacggt catgggtctg ttttccgttt
     4861 ctttcggtac ttgtcggaat cccgtgctct cggcccgtgt tttcgccggg agcggcgctt
     4921 ccgtcttaat tgtaatcctt ggggtgtcga aaaaaaccct ttcgggcccg gtgccgatgg
     4981 ttgccggccc tcgagtgctc ttggcgggct caaacctcgt gtcatgatag ggaaaaccca
     5041 gtgggagtca aaaggaaaaa ttgtctggaa atcaggagtc tttcggacaa tggtaagaaa
     5101 tcaaaggaaa aagtgcgctg tgtgggcgag gccgtccacg cggatgggag aagagggagg
     5161 ctcccgataa tctcctcgct tcccgggcgg cgagtccggg aggacggatt gtcgagggag
     5221 tgcattcctc ctcgtcgtct acggaaggtt ccatgcgggg ggcgggtttg ctcatttacc
     5281 ggatgaattg gacctatact atatcgaaat gatatcgtat catcgtctcg tgaacaggac
     5341 ggaaaggctg actcacccat gtctgaggac ccacggtcgg gggaatcgtc ggccggggct
     5401 gccgaggaga cacgaaggga agggaggtga acggtgaggc tgtccacgcg cagtcgctat
     5461 ggaatgcgcc ttctgttgga tatggcctgg cattacggcc gggggcccat ccagctggcc
     5521 gtcattgcca aacgtcaggg tattccactg aaatacttgg aacaaatcgt tattcccctc
     5581 aagaaggccc attacgtgca gagtgttcgg ggtcccaggg gggggcacat gctggccaaa
     5641 tcccccagtg aaatcaacgt cggggagatt gtgttcctct tggagagaaa agcgcagctg
     5701 gtggaatgtg tgaacaaccc cgaggtctgt gaacgctcat cgacttgtgt gacccgcacc
     5761 ttgtggaaag agacaacgga ggccatctgc gagaagctca acagcatcag cctttcggat
     5821 atcatggaac gctctcccac ggagctcgat ctttaagccc gccaggtttt cggctcaccc
     5881 ggcagatgat aggaagttgc attcaagccg aaactgccca tccgcaggac tcagcccttt
     5941 actcgaaatc tttacccagc gtgtcagggc gactttgggc gcaacctggc atgagtaccc
     6001 cggggttgaa aaaagggaat ggacacggca aagccccaac ttgtttgacg cccatcaaac
     6061 aggaagggag aacaagccgt gtccacgtcg ataccgtacc gtgctttggg gctgaagggt
     6121 atttgccgga cctcgactca ccaccgtgcc gatgcg
//
LOCUS       DAOOVC010000198         6087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7492_length_6087_cov_5.560179, whole genome shotgun sequence.
ACCESSION   DAOOVC010000198 DAOOVC010000000
VERSION     DAOOVC010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_6087_cov_5.560179"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="rseP"
                     /locus_tag="PLM79_16535"
     CDS             <1..696
                     /gene="rseP"
                     /locus_tag="PLM79_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOV87965.1"
                     /translation="VAAGSPAEQGGLQVGDRVISINDKKLETWENLSGTIEHSTEETL
                     VLKVQRGEETLTLPVKPMVSEAKNLFGETVTRRLIGVTPSGKIQVRKVDPFLAGYYSL
                     VQTYHLSRLFLLTVVKLIERVVPFHTLGGPILIAQMAGQQAQEGLLNLIYFIAVISVN
                     LAVLNLLPIPILDGGHILFFTLEAVLGKPIGIRKLEMAQKVGMVLLLVLMVFVFYNDI
                     MRLIPGHKPDILP"
     gene            716..1417
                     /gene="tsaB"
                     /locus_tag="PLM79_16540"
     CDS             716..1417
                     /gene="tsaB"
                     /locus_tag="PLM79_16540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOV87966.1"
                     /translation="MKILAADTSTPSGSVAVMEGDRLLAEWTFHSGHTHNRRLLKTLD
                     SVLRELGWSLDGVEAFAVTAGPGSFTGLRIGLTTIKTLAWSTGKPYVGISSLDVLAAP
                     LAYSSHPVCAVLDAKKQQVYAALYRGNQRGEPLRASPYQVMGPGELPGRLTEPTILVG
                     DGWLLYRDLFRQAMGESALEPPPAFHTLRAGFLADLAQRRLLCGEAQDPVSSVPLYVR
                     PSEAELRLSPAPAPA"
     gene            1566..2108
                     /locus_tag="PLM79_16545"
     CDS             1566..2108
                     /locus_tag="PLM79_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87967.1"
                     /translation="MHFPLTFFLRNVAAVRKEFQAGKVPARVQRRTLMRLLRQRISCY
                     SQVKSWLKGAGIRPDRIFGHPEVGHSSSPYCNHCGGCCEIPGGMPDFPGESPLPAKGR
                     EWFGEGLGPGHRFCPFLWESGETGRSFCSIHPWRPNPCRIFDHEECAVVKGDPDFTAS
                     CRPEQLLETCRWLVRLLDGR"
     gene            complement(2061..3089)
                     /locus_tag="PLM79_16550"
     CDS             complement(2061..3089)
                     /locus_tag="PLM79_16550"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HOV87968.1"
                     /translation="MVKSRWKTGTLVVATILFFGGLLWMESTAWARAGGGGSSGSRGS
                     RSFSTPRAPSSPSPSAPRQTPSPGAQFPGSPTQPGSGGWFSRSPFLQGMAGGLAGGLL
                     GNMLFGGRGYASPMGGGGGGGIGLLDLIIIGLLIYFGWRFFKKRRAAMNSADPYGPRY
                     DAASGRPFEGSPYYGGAEPEPVQAYDEVERGFAQIRQFDPGFEEESFKEKVQDLFFRI
                     QAAWMNRSLDGIENLVTQEMADYFRKEFASMKQQGIINRLENIAVRKVEPSEVWQEMG
                     KDYVTVLFTANLLDYTVDEKTNQVVQGDRLNPVKFQEFWTFVRDIGSPQWKLSAIQQA
                     DQPPARLQ"
     gene            complement(3429..4229)
                     /locus_tag="PLM79_16555"
     CDS             complement(3429..4229)
                     /locus_tag="PLM79_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV87969.1"
                     /translation="MTREKVIDRAKELLGEGKIKGFIALRERNGHVQPHIFTTVEELT
                     ALNLGDKKRPGDARYPLNKLLVELSRLYPEGTFAVLVRGCDERGIVELVKWHQLDPER
                     LVTVGIPCAEELARACACSKPYPDNSVEGEKVQPASRSPLLEGVEGKSLEDRFQFWME
                     HFARCVKCYGCRNICPMCFCQECSLEEGELIQKGVLPTENPIFHLTRAVHMIGRCIDC
                     GLCEEACPADIPIRALYKKVAEIVAAETGYRPGENPDEKCPYNVYGVS"
     gene            complement(4283..4723)
                     /locus_tag="PLM79_16560"
     CDS             complement(4283..4723)
                     /locus_tag="PLM79_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4670..4672),aa:Sec)
                     /transl_except=(pos:complement(4517..4519),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOV87970.1"
                     /translation="MSHTKSEPKILCFLCTWUAYAAADLAGVSRLQYPTNIRIIRMMC
                     SASVSPHHILRAFQEGVDGVFVGGURLGDCHYLYGNYSTKRRVGFLAELLAFAGIDPR
                     RIRSRWVSSAEAPEFAHEIKEFVQTLRELGPSPLGRRNQADEAA"
     gene            complement(4767..>6087)
                     /locus_tag="PLM79_16565"
     CDS             complement(4767..>6087)
                     /locus_tag="PLM79_16565"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV87971.1"
                     /translation="MAKARDLIRMGVAKATFLEPQQDIESSLVRRAMVIGGGVAGLSS
                     AEALAGMGVEVVLVEKESALGGLLRNLHRLAPGNDRASERIGALIQRVQASPNVQIFL
                     ESKVVDVGGYIGNYDLVVQDRDGQTHPFTVGCVVVATGAVPWQPDGLFGYNGKTVITQ
                     YELEGLLENEAFRAKNMVMIQCAGARSPERTYCSRICCLTAVKNALFVKERSPDTHVH
                     VLYRDIQMYGTENERLLWESRGKGVRYDVYDATRPPVVKEGLVEFYQPLTGEVVEIPC
                     DLVVLSTPLVARPEAPALAQLFRIPADQNNFFLEAHAKLRPLDFATDGIFLAGSARYP
                     ATTAEARAQGLGAASRAGTVLFKDKLVSSALVADINPNTCVGCQGCLGVCPYGAITYV
                     WEERICRVNTILCKGCGNCASTCPSQSARLKGFTPRQLLSQIRAVVA"
BASE COUNT         1162 a         1944 c         1800 g         1181 t
ORIGIN      
        1 gtggccgcag gctctcccgc cgaacagggg gggctccagg tgggagaccg ggtgatcagc
       61 atcaacgaca agaagctgga gacctgggag aatctttcgg gaaccatcga gcattccacc
      121 gaggaaaccc tggtattgaa ggttcagcgg ggagaggaga ccctcaccct ccccgtgaaa
      181 cccatggtca gtgaagccaa gaatctcttc ggggaaaccg tcacacggcg cctcatcggg
      241 gtcactcctt cgggaaagat ccaggttcgc aaggtggacc cctttctggc aggctactac
      301 agccttgttc aaacctacca cctgagcagg ctgttccttc ttaccgtggt caagctcatc
      361 gagagggtgg tgccctttca caccctgggc gggcccattc tcatcgctca aatggcggga
      421 cagcaggccc aggaagggct gctgaacctg atctacttca ttgccgtgat cagcgtgaac
      481 ctcgcggtgc taaacctttt gcccattccc atcctggacg gaggccatat ccttttcttc
      541 accctggaag ccgttctcgg caaacccatc ggaatccgga agctggaaat ggcccagaag
      601 gtgggaatgg tcctgctcct ggtgctcatg gtcttcgtct tctacaacga catcatgcga
      661 ctgatcccgg gccacaagcc ggatatcctg ccctagacgc aacggtgccc ctagaatgaa
      721 gatcctggcc gcagacacct ccaccccttc gggcagcgtg gcagtgatgg agggagaccg
      781 tctcctcgcc gagtggacct tccactccgg ccatacccac aatcgtcggt tgctgaagac
      841 cctggactcc gtgctgcggg aattgggatg gagcctcgat ggggtggagg cctttgccgt
      901 gaccgcgggc ccgggaagct tcacggggct gcgcatcggt ctgaccacga tcaagaccct
      961 tgcctggagc acaggcaagc cttacgtcgg gatctcctcc ctggatgttc tggccgcccc
     1021 tttggcctat tcttcccatc ccgtttgcgc cgtcctggat gcgaaaaaac agcaggtgta
     1081 tgcggccctc taccggggaa accagagggg ggagcccctg cgggcttcgc cctatcaggt
     1141 catggggccc ggggaactgc ccggtcggtt gacggagccc accatcctgg tgggagacgg
     1201 gtggttgctg taccgggacc tgtttcggca agccatgggg gaatccgccc tcgagccgcc
     1261 cccggccttt cacaccctac gcgccgggtt cctggcggac ctggcccaaa gaaggcttct
     1321 ttgcggagaa gcccaagacc ccgtgagcag cgttcccctc tacgtgcgcc cttccgaggc
     1381 ggagctccgc ttgtccccgg cacccgcccc ggcttgaccc tccccagaca ggatgcgaat
     1441 cggtggaagc gatccccgaa ggtgccctct accccccgca catccgccga caatcccggc
     1501 gaatgtggca aaccgtccgc tcccggtggc cgggatcacc ggcagtcctt cggaaaaccg
     1561 catgcatgca ctttcccctg acgttctttc tccgcaatgt tgcagccgta cgaaaagaat
     1621 ttcaggccgg gaaggttccc gcacgggtcc agcgccggac cctgatgcgg ctcctccggc
     1681 aaaggatctc ctgctattcc caggtgaaga gctggttgaa aggggcggga attcgcccgg
     1741 accggatctt cgggcacccg gaggtcggcc actcgtcttc cccgtactgc aatcactgcg
     1801 gcggatgttg tgaaattccc ggcggaatgc cggattttcc cggggagagc cccctacccg
     1861 cgaaggggag ggagtggttt ggggaggggt tgggccccgg ccatcgcttc tgcccgttcc
     1921 tctgggaatc cggagaaacg ggccgaagct tctgcagcat ccatccctgg aggcccaacc
     1981 cgtgtcggat ctttgaccac gaagagtgtg ccgtggtgaa gggcgacccg gattttacgg
     2041 cttcctgtcg cccggagcaa ttactggaga cgtgccggtg gttggtccgc ctgctggatg
     2101 gccgatagct tccattgggg cgatccgatg tcccgaacaa acgtccagaa ctcctggaac
     2161 ttcaccggat tgagccgatc cccctggacc acctggttcg ttttctcgtc tacggtgtag
     2221 tccaggaggt tcgccgtgaa caggaccgtc acgtagtctt tgcccatttc ctgccacact
     2281 tcggaaggtt ccactttccg aacggcgatg ttctccagac ggttgatgat cccctgctgc
     2341 ttcatggatg cgaattcttt gcggaagtag tccgccattt cctgggtgac caggttctcg
     2401 atgccgtcaa ggcttcggtt catccaggcc gcctggatgc ggaaaaagag atcctggacc
     2461 ttctctttga agctttcttc ctcaaacccg ggatcgaact gccggatctg tgcaaagcct
     2521 ctttccacct cgtcgtaagc ctggaccggt tccggctccg ctcccccgta atagggcgat
     2581 ccctcgaaag gacggcccga ggcggcatcg tatcgagggc cgtacggatc ggcggaattc
     2641 atggccgccc ggcgtttctt gaagaacctc caaccgaagt agatgagcaa tccgatgatg
     2701 atcaggtcca agagaccgat gcccccgcca ccgcctccac ccatgggaga ggcatagcct
     2761 cttccgccaa aaagcatgtt ccccagaaga ccgcccgcga ggcctccggc catgccctgg
     2821 aggaacgggc ttcggctgaa ccaccccccg gagccgggtt gagtggggct tcccgggaac
     2881 tgggcgcctg ggctgggggt ttgacgggga gcggagggag aaggagagga aggtgcccgt
     2941 ggagtggaaa agctgcggct gccgcggctg ccggacgatc caccccctcc ggctcgagcc
     3001 cacgcagtgg actccatcca gagcagccct ccgaaaaaca agatggttgc aacgaccaag
     3061 gtacccgttt tccatcgact cttcaccatt cccgtctcct cacccagtgc attcaaagtt
     3121 ctctgccgcc cgacttccaa ccagatcggc ggcgcttctc tcgacgattc acgaaaatca
     3181 aagtaacaaa ggaggattca gaaggcaata gggatcggcc cggggaggcg cccaagcaag
     3241 cacggcggcg ggacttgggc gcccccttcc cgggatgcgg cagggatggt tgggagcgcc
     3301 cgccccgagg tccttggggg ggccggaaaa acggcgggcg gagcgcacgg gggctttgct
     3361 tccgggcatc ctcgcccttc cccccgtgcc ctctctgcaa tgaaatgcgc gacttgaccc
     3421 gggaaaccct acgaaacccc gtagacgttg taggggcatt tttcgtccgg gttctccccg
     3481 ggccgatatc cggtctccgc ggcaacgatt tcggccacct tcttgtacag ggcccggatg
     3541 ggaatgtccg cagggcatgc ctcctcgcag aggccgcaat cgatgcagcg gccgatcatg
     3601 tgaacggccc tcgtcaagtg aaagatggga ttttcggtgg ggagcactcc cttttggatt
     3661 agttcgccct cctcgaggct gcactcctgg cagaaacaca tggggcagat gtttcggcac
     3721 ccgtagcact tcacgcagcg ggcaaagtgc tccatccaaa actggaaacg atcctcgagg
     3781 gacttgccct cgacgccttc cagcagcgga ctccgagaag caggctgaac cttttccccc
     3841 tcgacggaat tgtccggata gggcttggaa cacgcacagg cccgggccag ctcctctgcg
     3901 cagggaattc cgacggtcac caatcgttcg ggatccagct ggtgccactt cacgagctcg
     3961 acaatccccc gttcgtcgca cccccggacc aatacggcaa aggtgccctc ggggtagagg
     4021 cgggaaagct ccacgagcag cttgttgaga ggatatctcg catccccggg ccgcttcttg
     4081 tcacccagat tgagggccgt caattcctcc acagtggtga atatgtgggg ttgaacatgg
     4141 ccgttgcgct cccgcagggc gatgaatccc ttgattttcc cttcgcctag aagctctttc
     4201 gctcgatcga tcactttttc tctggtcatc ggctcttatc tcttggtgga ggctccatgg
     4261 tttttcgcac gccctctgcc ggctatgcgg cctcgtccgc ctgattcctg cggccgaggg
     4321 gcgatgggcc caactcccga agcgtttgga cgaattcctt gatctcatgg gcgaattccg
     4381 gcgcttcggc cgacgagacc caccgggaac ggattcttcg aggatcgatt cccgcaaaag
     4441 ccagcagctc tgcaaggaat cccacccgcc ttttggtcga gtaattaccg tacaggtaat
     4501 ggcaatcccc cagccgtcaa ccgcccacaa acaccccgtc cacaccctcc tgaaaagccc
     4561 gcaggatgtg gtggggggag acgcttgccg agcacatcat ccgaatgatg cggatgttgg
     4621 tcgggtactg caaacgactg accccagcca ggtcagcagc ggcgtacgct caccaggtac
     4681 agaggaaaca cagaatcttg ggttccgatt tcgtgtggct cattccaata caccccttcc
     4741 taggtttggg accggcaatt cgacaatcaa gccacgacag cccgaatctg ggaaagaagc
     4801 tgtcgagggg tgaacccttt caaccgggcg ctctgagacg gacaggtgga agcgcagttg
     4861 ccgcatccct tgcacaggat ggtgttcacc cggcagatgc gctcctccca cacataggtg
     4921 atggccccgt agggacaaac ccccaggcat ccctggcatc ccacgcaggt gttgggattg
     4981 atgtccgcca ccagggcact ggagaccagc ttgtccttga aaaggacggt gcccgcccgg
     5041 gaagccgctc ccagcccttg agcccgggct tccgccgtcg tggcgggata tcgggcggac
     5101 ccggccagga agatgccgtc cgtggcaaaa tccaacggcc tcagtttggc atgagcttcc
     5161 aggaagaagt tgttctggtc cgcggggatc cggaacaact gggccaaggc gggagcctcg
     5221 ggtcttgcca ccaggggcgt ggagagcacc accaggtcgc acgggatttc caccacctcc
     5281 cccgtaaggg gctgatagaa ctcgaccagc ccctccttca ccacgggagg ccgggtggcg
     5341 tcgtagacgt cgtagcgcac cccttttccc ctggattccc agagcaggcg ctcattttcc
     5401 gttccgtaca tctggatgtc ccggtagagg acatgaacgt gggtgtccgg agaccgctct
     5461 ttgacgaaaa gagcattctt tacggccgta agacagcaga tcctggagca gtacgtccgt
     5521 tcgggagacc gcgccccggc acactgaatc atcaccatgt tcttggcgcg gaaggcctcg
     5581 ttttccagca gcccttcgag ctcatactgg gtgatgacgg tcttgccgtt gtagccgaag
     5641 agaccgtccg gctgccaggg aaccgctccc gtcgccacca ccacgcaacc gacggtgaag
     5701 ggatgcgttt ggccgtcccg gtcttggacc accaggtcgt agttgccgat atagcctccc
     5761 acgtccacca ctttgctttc caggaatatt tgcacgttgg gtgaagcctg gacccgttgg
     5821 atgagggccc cgattcgctc ggaggcgcgg tcgtttccgg gagccagtcg gtggaggttt
     5881 ctcagcaagc cgcccagggc gctttccttt tccaccagca ccacctcgac tcccatgccc
     5941 gccagggctt cggcggaaga gagccccgcc accccgcccc caatgaccat ggctcgccgc
     6001 accagggaag actcgatatc ctgctggggt tccaggaaag tggccttggc cacccccatg
     6061 cgaatgagat cccgggcctt ggccatg
//
LOCUS       DAOOVC010000199         6015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7592_length_6015_cov_5.923490, whole genome shotgun sequence.
ACCESSION   DAOOVC010000199 DAOOVC010000000
VERSION     DAOOVC010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7592_length_6015_cov_5.923490"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..1116
                     /gene="trpS"
                     /locus_tag="PLM79_16570"
     CDS             112..1116
                     /gene="trpS"
                     /locus_tag="PLM79_16570"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOV87972.1"
                     /translation="MSERKRILSGMRPTGRLHLGNLHGALDNWIRLQSQYECFFFVAD
                     WHALTTDYASPGQITENTWDMVLDWLSAGLDPRQATLFIQSEVKEHAELYLLLGMITP
                     LPWLERNPTYKEQQQQLDQKDLATYGFLGYPVLQAADIIIYRAHGVPVGKDQIPHVEM
                     TREIARRFNFIYQREVFPVPDVLLTEAPVLPGTDGRKMSKSYGNCIYITEPGENVREK
                     ILKMMTDPARVRRNDPGNPELSPVFALHKLYSSPAEIEEVAEGCRTARIGCIDCKKIL
                     IKNVLARLDPIREKRMELEGKPDQVRETIRQGISSAQKVAAETIAEARRAIGIDPMRT
                     "
     gene            1163..1912
                     /locus_tag="PLM79_16575"
     CDS             1163..1912
                     /locus_tag="PLM79_16575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOV87973.1"
                     /translation="MKGIRLQKVLAQAGLGSRRTAEEIIRQGRVAVNGSIVRELGTRV
                     RPETDDIRVDDAPIGLESRKAVLLFNKPGNCVTTLRDPQGRKTVMDYLPPLGIRLFPV
                     GRLDYDAEGLLLLTNDGLLAHRLQHPRHGVSKIYEVKVRGIPGEEALIRLRSGVPLAR
                     GVTAPAQVSVLRHSSKTSWLKMVLHQGWYRQIKQMGEAVGHPVLKIRRVGYGPLRLGD
                     LKPATFRYLTSKETRRLYGMLGMDEELRQEP"
     gene            1909..3789
                     /locus_tag="PLM79_16580"
     CDS             1909..3789
                     /locus_tag="PLM79_16580"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV87974.1"
                     /translation="MSPKRHGGYSYSLDESVTRSAKRKKQSHFLSVVLWLLLLTVVGA
                     AGLFVFYPSLLSTATSYLPFPKELVAIRLLHNGKEVILLPDTQCLVNPRDSLQLLQVK
                     TDGWVSWGTKVVATDLDVRAIRAQARTLRELMPNESFETPKTVELRALLWNRPIGKVS
                     FLVQLDAKDWLQKANTTADMDRKITYLEKALRENSGNVLVKTQLATLYFDSKRYDEAV
                     QLYKEINEVGKTKPVLERLLTIYQAQKRVDDALMTYLELLRLSEDREEFKEFLGYLQK
                     NKSKEEAGKFLEKHQQEVPKAFHSSLLLVLAELNTQTKNWAKAAATYEKVLKSGVKDP
                     DVYYNLAVTYQHSEDPDKAIHALERYLQKNPADLRSWMQMGELQERKKDYGGARRTYE
                     SVLQKNPGNKEALVRMIAVLEKTNDKAALQAGYEQLVQLQPKNKTLLHNLGVLYYEGK
                     KWDKAADAFEKIASLDPKDVESRKYLLDIYRKQKNTKAEMETLGEMARLDPGNTATLD
                     SLFKAYDSKKDYKGMVAFFRGVAQKKPDSVPVHNYLLYGLLKTGDNKGALKELEELAR
                     LQPKEKKHLRQAARLYESAGNTSEALKKLDQLLKLDPQDKEAKDDYLRLRMQGLSKKK
                     QP"
     gene            4044..4119
                     /locus_tag="PLM79_16585"
     tRNA            4044..4119
                     /locus_tag="PLM79_16585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4077..4079,aa:Val,seq:cac)
     gene            complement(4183..4551)
                     /locus_tag="PLM79_16590"
     CDS             complement(4183..4551)
                     /locus_tag="PLM79_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87975.1"
                     /translation="MEQPRTRSHRKLGNVRDHGGGGKPKGAPFGKHILYSYWKKACSN
                     LGIEGVDLYAGTRHSTMQYMRILGHSPEEVKRLSFHTTNAAMNRYLQINPEELRAGYA
                     QVRKPLLGAVVPIKPNKFGL"
     assembly_gap    4619..4718
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4907..5338)
                     /locus_tag="PLM79_16595"
     CDS             complement(4907..5338)
                     /locus_tag="PLM79_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87976.1"
                     /translation="MDKPRKHTVSPRISTKTKEFYESRFDSLNSGVTYAIESFPTLFR
                     SSLHETKGKFKSNELMFILDVMSSTTINPELIGQHLSYNLADAIHFDQHHTKWGVDAD
                     AFYPKVVALTRAQAAAIELWAYFYHMIDNQDLLEHWCHTLL"
     gene            complement(5416..>6015)
                     /locus_tag="PLM79_16600"
     CDS             complement(5416..>6015)
                     /locus_tag="PLM79_16600"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HOV87977.1"
                     /translation="PDSVEFYDKGERILRYDQHRSRHGIEVSMLHRIALNSTDKWVLV
                     KYCRGGTSSYAWRPDWTRELADITNDADKGNLYIETIKAIRAAEKSHGQPIIWSHILA
                     INGERESRFRQAAHDYTKNMIKIRNALNRDLHASATLIVLTLDLQDSKARPWTSLVRN
                     QQEQMTHVHASGCQRIGIHYDAQGLARIGNRFSQHILAN"
BASE COUNT         1262 a         1623 c         1766 g         1264 t
ORIGIN      
        1 cgggatactg atcctcttgt tgctcattgc cactagggcc tgggaccact ttgtgcgccc
       61 ggtcttgaat ttattcgtga gactaattct ttaggaagga gttcaacatc gatgtcggaa
      121 cgaaagcgga ttttgagcgg catgcgccct acggggcgcc ttcacctggg gaatcttcac
      181 ggagcactgg acaactggat tcgacttcag tcccaatatg agtgtttctt cttcgttgcc
      241 gactggcatg ctctgaccac cgattacgca agtccagggc agattaccga gaacacgtgg
      301 gatatggtcc tggattggct cagcgccggg ctggaccccc ggcaggccac cctgttcatc
      361 cagtccgagg tgaaggaaca tgcggagctc tacctgctcc tggggatgat cactcccctg
      421 ccctggctcg agcgaaaccc cacctataag gaacagcagc agcagctgga tcagaaggac
      481 ctggccacct acggctttct gggctacccg gtgctccagg ccgccgacat catcatctat
      541 cgcgcccacg gcgttcccgt gggaaaagac cagatccccc acgtggagat gacccgggag
      601 atcgcccgcc gtttcaactt catctaccag cgcgaggtct tcccggtccc cgacgtgctc
      661 ctcaccgaag cccccgtcct gccgggaacg gacggtcgga agatgagcaa gagctacggc
      721 aactgcatct acatcacgga acccggggag aacgttcggg agaagatcct gaaaatgatg
      781 acggacccgg cccgggttcg aaggaatgat ccggggaacc ccgagctttc gcccgtcttt
      841 gccttgcaca agctgtattc gtccccggcc gagatcgagg aagtggccga aggctgtcga
      901 acggcccgca tcggctgcat cgattgcaaa aagatcctca tcaagaatgt tctcgcgcgg
      961 ctggacccca ttcgagaaaa gaggatggaa ctcgagggga agccggacca ggttcgggaa
     1021 accatccggc agggcatttc gtcggcccaa aaggtggcgg ccgaaaccat tgccgaagcc
     1081 cgcagggcca tcgggatcga ccccatgagg acctgaaggc gggagtcatg acgaggggac
     1141 ccattcgaga cggtgaggtt ccatgaaggg gatccggctt cagaaagtgc tggcccaggc
     1201 ggggctgggc tcccgtcgaa cggcggagga aatcattcga cagggccggg tggcggtcaa
     1261 cggatccatt gttcgggaat tgggaacccg ggtccgcccg gagacggacg acatccgggt
     1321 ggacgacgca cccatcggcc tcgagtcgcg caaagcggtt cttcttttca acaagcccgg
     1381 caactgcgtc accacccttc gagaccccca gggcagaaag accgtgatgg actaccttcc
     1441 ccccctgggg attcgcctct tcccggtggg acgcctggac tacgatgccg agggattgct
     1501 gctgctcacc aacgacgggc tgctggctca ccgattgcaa catccccgcc acggcgtctc
     1561 caaaatctat gaggtaaagg tccggggaat tcccggcgag gaagctttga tccggcttcg
     1621 gtccggggtc cctctggcca ggggcgtcac cgcccccgca caggtatccg ttcttcgtca
     1681 ttcgtccaag acctcgtggc tgaaaatggt gctgcatcag ggctggtacc gtcagatcaa
     1741 gcagatggga gaagcggtgg gccatcccgt tctcaaaatc cgaagagtgg ggtatggtcc
     1801 cctcaggctg ggagatctca aacccgcaac gttccgctat ctgacctcga aggaaacccg
     1861 gcggctctac ggaatgttgg gaatggatga ggagctgagg caagagccat gagtcccaaa
     1921 agacacggcg gttattctta cagcttggat gaatccgtca cgcgttcggc aaagcgcaaa
     1981 aagcagtcgc acttcctgtc cgtcgtactg tggcttctgc tgctgaccgt ggtgggggct
     2041 gcaggcctct ttgtctttta ccccagtctc ctgtcgacgg cgacctccta cctgcccttt
     2101 cccaaggaac tggtggccat ccggctcctt cacaacggca aggaggtcat cctcttgccc
     2161 gatacccagt gcctcgtcaa cccgcgggat tctttgcagc tcctccaggt caagaccgac
     2221 ggctgggttt cctgggggac caaggtcgtg gccacggacc tcgacgtgcg cgccattcgg
     2281 gcgcaagccc ggactcttcg ggagctgatg cccaacgaat ccttcgaaac gcccaagacc
     2341 gtcgagcttc gagctctgct ctggaaccgt cccatcggca aggtctcctt cctggttcaa
     2401 ctggacgcca aggactggct ccaaaaggcc aacaccaccg cggacatgga ccgcaagatc
     2461 acgtacctgg aaaaggccct ccgggaaaac tccggaaacg tcctggtcaa gactcagctg
     2521 gcgaccctgt acttcgattc caagcgttac gacgaggcgg tgcagcttta caaggagatc
     2581 aacgaggtcg gcaagaccaa gcccgtcctg gaacggctcc tcaccatcta tcaggctcaa
     2641 aagcgggtcg acgacgccct gatgacctac ctcgaactcc tgcgactgtc cgaagaccgc
     2701 gaggagttca aagaatttct cggctacctg caaaagaaca agtccaagga ggaggcgggg
     2761 aagtttctcg agaagcacca gcaggaggtg cccaaggctt ttcacagctc cctgctcctc
     2821 gttttggctg agctcaatac ccagaccaag aactgggcca aggcggcggc cacctacgaa
     2881 aaggtcctca agtccggggt caaggacccg gacgtgtact acaacctggc cgtgacctac
     2941 cagcacagcg aagacccgga caaggccatc cacgccctcg agcgatatct ccagaaaaac
     3001 ccggcggacc tgcgcagctg gatgcagatg ggagagctcc aggagcgtaa gaaggactac
     3061 gggggagcgc gtcggaccta tgaatcggtc cttcagaaga atccgggcaa caaggaagcc
     3121 ctggtgcgaa tgatcgccgt gttggaaaag accaacgaca aggcggccct gcaggcgggc
     3181 tacgaacaac tggttcagct gcagcccaaa aacaagaccc tgctccacaa cctgggggtc
     3241 ctctattatg aaggcaagaa atgggacaag gccgccgacg ccttcgagaa aatcgcctct
     3301 ctggacccca aggacgtgga atcccgcaag tacctcctcg acatctaccg caagcagaag
     3361 aataccaagg ccgaaatgga aaccctaggg gagatggccc gcctcgaccc ggggaacacc
     3421 gccaccctcg attctctttt caaggcttac gattccaaga aggactacaa ggggatggtt
     3481 gcgttctttc ggggagtggc gcaaaagaag cccgattccg tcccggtcca caactatctg
     3541 ctctacgggc ttctgaaaac cggggacaac aagggcgcct tgaaagaact cgaagagctc
     3601 gcccggctcc aacccaagga aaagaagcac ctgcggcagg cggcacgcct ctacgagtcc
     3661 gcgggcaata cctcggaagc cttgaaaaag ctggatcaac tgctcaagct ggacccccag
     3721 gacaaggaag ccaaggacga ctacctgcga ttgcgcatgc aggggttgtc caagaagaaa
     3781 cagccgtgat ccgtcggggt cgagggcgcg tcccggaacc aatggggtcg aggcccgggg
     3841 cgggccgggg agcacgggag ggatttttcc cccgcccggc ggcgccccgg gattcgagat
     3901 ccgttccatt ccccccgagg gcggaaagcg ggaagggatg gaaaaccgcc cggcaaaatc
     3961 cacccgcgca ccgggacttt gctgttgcag tcccggcggt tttgttgtat cagtgtctcc
     4021 gtttcgaggt tccatcgaag cgtgggcggg taactcagcg gtgagagtgc catcctcaca
     4081 cggtggaggt cggaggttcg aatcctctcc cgcccaccaa gaaaacgaat aaaatcaatc
     4141 atttgtgcga ctccaagagt gaagaaaatc gttacaaatg cgttacaggc cgaatttatt
     4201 gggttttatt ggtacgacgg ctccgagcag cggcttgcgc acttgtgcgt agcctgctcg
     4261 gagctcttcg gggttgattt gcaaatagcg gttcatggct gcgtttgtgg tgtggaagga
     4321 gagccgtttg acctcttccg gactgtgccc gagaatgcgc atatactgca tggtgctgtg
     4381 tctggttccc gcgtaaaggt cgaccccttc gatgccgagg ttggagcagg cctttttcca
     4441 atatgaatag aggatgtgtt ttccgaacgg agcccctttg ggctttccac cgcctccgtg
     4501 gtctctaacg tttcccagct tgcgatgaga tcgagtcctt ggttgctcca aggatcgatg
     4561 cctatccttt caagatcgag ttcaattctc catacggatt tgtctgagaa gagcacgtnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat cctttcaaga tcgagttcaa
     4741 ttctccatac ggatttgtct gagaagagca cgtttccgtt ctgggtgaca taagctccca
     4801 ggaatccttc cgggttgtcg gcgatgcgtc cgaggagcca tacggagtcg tagtcgtcgg
     4861 cgaagtcgtg ttccacgatg acgaatttgg tcatgtgttg tgtcctttag aggagggtgt
     4921 ggcaccagtg ttccagcagg tcttggttgt cgatcatgtg gtagaagtat gcccaaagct
     4981 cgatggctgc cgcttgtgct ctggtgaggg cgacgacttt cgggtagaag gcgtccgcgt
     5041 ctacgcccca tttggtgtga tgttgatcga aatgtatggc gtcggcgagg ttgtaggaca
     5101 ggtgttgtcc gatgagttcc gggtttatgg tggttgagct catcacatcc aggatgaaca
     5161 tgagttcgtt gcttttgaat tttcctttgg tttcgtggag agaggatcgg aagagggttg
     5221 ggaaggactc gattgcgtag gtgactccgg agtttaagga atcgaagcgg ctttcgtaga
     5281 attctttggt ttttgtggat attcttggag atacggtgtg tttccttggc ttgtccatga
     5341 tgccccccca ttttctgttg tatgtcgtga tattatgata gagaaatgct ttgcaaatgt
     5401 caattcaaaa tgtggttaat tggcaagaat atgttgtgag aaacggtttc cgatccgtgc
     5461 gagtccttgg gcgtcgtagt ggatgccgat tcgttggcag ccggaggcat ggacgtgggt
     5521 catttgttct tgttggttgc gcacaaggct tgtccatggg cgtgctttgg agtcctgcaa
     5581 atcgagggtg agcacgatca aggttgcgga tgcgtgcaga tctcggttca gtgcgttgcg
     5641 gatcttgatc atgttcttgg tgtaatcgtg ggcggcttgg cggaagcggg attcgcgctc
     5701 tccgtttatt gcgaggatgt ggctccaaat gattggttgg ccgtggcttt tttcggctgc
     5761 acggattgct ttgatggttt cgatgtagag gtttcctttg tccgcgtcgt ttgtgatgtc
     5821 ggcaagttcc cgagtccagt ccggacgcca tgcgtacgat gaggttccgc ctcggcagta
     5881 ttttacgagg acccatttgt cggtggaatt cagtgcgatg cggtgaagca tggacacttc
     5941 gattccgtgt ctggatctgt gttgatcgta tcggaggatt cgttctcctt tgtcgtagaa
     6001 ctcgacggaa tcggg
//
LOCUS       DAOOVC010000200         5952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7672_length_5952_cov_6.570968, whole genome shotgun sequence.
ACCESSION   DAOOVC010000200 DAOOVC010000000
VERSION     DAOOVC010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5952
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7672_length_5952_cov_6.570968"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLM79_16605"
     CDS             <1..1609
                     /locus_tag="PLM79_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87978.1"
                     /translation="DCQGSDSPNPLHFALLQQHLLGTHLVLYVISSRYGLREADFRLL
                     EFIRTLRLDPHTLFVLNVDLDVHPDLEDIEAMCGRVKSELEWVVPDPQLFRFSALLHL
                     ADQVGDALPEKERRLLRHWKKEKALVRKSSQGFQGFRDRMAERIQRQRTDTLLRSILN
                     RLSLVAGSVLDTVKTRKAFLDQDIEVLKESSGPLREKHASMQGMLLTLRNSISGLREA
                     LLQDLDGSADAFFHPTAGMIVPETMELVERFPMDPRVHRELTDLRQALRQLYRVYLEF
                     REGITRFLVERINTRVIEFAREREAFLQARLQQAYESFWTLFQTAVEEYRGGLAQYGV
                     SLEKFEGSRGWDGAFLGRIQPAPFSAAASRAVTRRGTLLLKFGLARLSGWLEDWKSSS
                     GNRGWGGLSRSLTGTPKVVEEAVALIKAETRSELGQSFAAYAGDFKVRYLRRMLDQGI
                     SQLHDEFEMRSEMARLDWTHLVDRSRAAGEDRTVAREALIRAGQILEAMKEELDRWSD
                     SPNPEEYPAEVPASREERPQGEPTAIR"
     gene            complement(1530..2456)
                     /locus_tag="PLM79_16610"
     CDS             complement(1530..2456)
                     /locus_tag="PLM79_16610"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOV87979.1"
                     /translation="MRKQAPDHGPGSPLSVTRWRAEDASRSVPLFQCLASRLKVSPEE
                     ASDLIDFGSVQVNGHRERKPARILEGREEIAVYWPWTGVRRHYEIDPRRIVFQDSCLL
                     AYDKEAGIPSQQTPSDAYNNVFAAAYRYREKLGGKDPYVALHHRLDRETSGILLLALD
                     REVNRHLGNAFRDRRVVKDYAAWVSGTPARDSWVSREPIGRKGGRYRTVPPGEGKPAE
                     TLFWVLSRRENGSLVRARPVTGRTHQIRLHLEAAGHPILGDRLYGGPPEKRLFLHAWC
                     LQLRHPVSGSPLVLPAAVPPDWPEPRPDIPPD"
     gene            complement(2453..2821)
                     /locus_tag="PLM79_16615"
     CDS             complement(2453..2821)
                     /locus_tag="PLM79_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOV87980.1"
                     /translation="MPIYEFRCGDCGKKTEIITLRVSEEVDPCCRHCGSRQVGRIPSR
                     VRVRLSEETRLERMADPSRLGGLDENDPKSMARWMKTMGQEMGEDLGEDVDALVEEAM
                     EAEARGELDPGGTAPEDDLP"
     gene            complement(2912..4111)
                     /locus_tag="PLM79_16620"
     CDS             complement(2912..4111)
                     /locus_tag="PLM79_16620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOV87981.1"
                     /translation="MESASPSTQLPGIVLHPGREKRILRGHRWVFSNEISTNPLSQFE
                     PGSWVEVFSGKGISLGSGYINPRSLIAVRILCPPGVRFSRDFLEERIREAVRRREEFY
                     YPGSSCYRAVFGESDGLPGLVVDRYGDVVVYQVNTLGMVRLEPWVRDLLLEIFRPAAL
                     VCRNDSSVRALEGLSPEKGVVHGGLPEDCRVSIDGIEYSLRPLEGQKTGFYLDQRDNR
                     RAFQRWTAGRRVLDLFCYNGAWSLAAVAGGAIEATGVDQSGDAVEQAAANAALNGLED
                     RCRFVREEAFHFLRSANKSDYDCIILDPPSFAKTRNALAAAKKGYTDLNRRALLLLPP
                     GGILVSCSCSYHVDEGLFEEILLQAAQASGRRLRLLEARTQAVDHPILLAMPETRYLK
                     CCFLEVF"
     gene            complement(4224..4496)
                     /locus_tag="PLM79_16625"
     CDS             complement(4224..4496)
                     /locus_tag="PLM79_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87982.1"
                     /translation="MEMKDFVKAALKKVNKKVAEGSLDKNEEGYDEPEEMLLDWIWIE
                     LKEESPDKDAVIGMDLDDLYEIIEGSADMYEDYQILLESLRKEEES"
     gene            4933..5586
                     /locus_tag="PLM79_16630"
     CDS             4933..5586
                     /locus_tag="PLM79_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOV87983.1"
                     /translation="MAEKQYVIDAMTVQDSWRLFKIIAEFVDGFQTLSDIYPAVSIFG
                     SARVKPGDPVYEKTVEIARKLAQNGFHILTGGGPGVMEAGNKGAREGGGRSVGLNIKL
                     PLEQEPNPYQNVRLDFQYFFVRKVMFIKYAQAYVGMPGGFGTMDEIFEALTLIQTRRI
                     KPFPVILVGKEYFHGLLEWVKNTLLSHNAISREDLEFLTLLDDPDEVVYTIKKYVIV"
     gene            complement(5765..>5952)
                     /locus_tag="PLM79_16635"
     CDS             complement(5765..>5952)
                     /locus_tag="PLM79_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HOV87984.1"
                     /translation="KDLAQVRGTFSIEVANYLLNQKRNELKRLEERYGTSIWIEGSPI
                     LLPHEGQIEFVSRDGAH"
BASE COUNT         1156 a         1818 c         1793 g         1185 t
ORIGIN      
        1 cgactgccag ggcagcgact ctcccaatcc cctccatttt gccctcctgc agcagcacct
       61 gttgggcacc cacctggtgc tctacgtcat cagcagccgt tacggcctgc gggaagcgga
      121 cttcagactc ctcgagttca tcaggaccct tcggctggat ccccacacgc tgttcgtcct
      181 caacgtggac ctggacgtgc acccggatct cgaagacatc gaagccatgt gcgggcgggt
      241 gaaaagtgag ctcgagtggg tggtgcccga tccccagctg tttcgcttct ccgccctttt
      301 acacctggcg gaccaggtgg gggacgccct gccggaaaag gagcgaaggc ttcttcgcca
      361 ctggaaaaag gaaaaggccc tggtgcggaa atcctcgcaa gggttccaag ggtttcggga
      421 ccggatggcc gagcggatcc agcggcaacg gacggacacc cttttgagaa gtattctgaa
      481 ccgcctctcc ctagtggcgg gaagcgttct ggacacggtg aagacccgga aggccttcct
      541 ggatcaggac atcgaggtgt tgaaggaatc ttcgggtcct cttcgggaaa agcatgcttc
      601 catgcagggc atgctgctga ccctccggaa ttccatttcg ggcctcaggg aggccttgct
      661 ccaagacctg gacggttccg ccgacgcctt tttccatccc acggccggaa tgatcgtgcc
      721 ggaaaccatg gaactggtgg aacgttttcc gatggatccc cgcgtacaca gagaactgac
      781 cgaccttcga caggcccttc ggcagttgta ccgggtgtac ttggaatttc gggaaggcat
      841 cacccggttt ctggtggaac gcatcaatac ccgggtgatc gagtttgccc gggaaaggga
      901 ggcgtttctc caggcgcgtc tgcagcaggc ctacgaatcc ttttggaccc tgttccagac
      961 cgccgtcgag gagtatcgcg gcgggctcgc tcagtacggc gtttccctgg agaagttcga
     1021 aggaagtcga ggttgggacg gagcgttcct cgggcgaatc cagcccgccc ccttcagtgc
     1081 tgcggcgagc cgagccgtca cccgtcgggg gacgttgctt ctcaagttcg gcctggccag
     1141 gttgtccggt tggttggagg actggaagtc ctcttcggga aatcgagggt gggggggatt
     1201 gtcccggagt ctcacgggaa cccccaaggt ggtggaggaa gcggtggccc tgatcaaggc
     1261 ggaaacccgc tccgagctcg ggcagtcctt tgccgcctat gccggggact tcaaggtccg
     1321 gtacctgcgc aggatgctgg atcaaggcat ttcacagctc cacgacgaat tcgagatgcg
     1381 ctccgagatg gcgaggctgg actggaccca cctcgtggac cggagcaggg ctgcggggga
     1441 ggatcggacc gtggcccggg aagccctgat ccgggcggga cagatcctcg aagccatgaa
     1501 ggaggaactg gaccgctggt ccgattctcc taatccggag gaatatccgg ccgaggttcc
     1561 ggccagtcgg gaggaacggc cgcagggaga accaacggcg atccgctgac gggatggcgc
     1621 agttgcaggc accaggcgtg caggaagagg cgtttttcag gggggccccc gtacaggcgg
     1681 tctcccagga tcgggtggcc tgcagcctcc aggtgaagac gaatctggtg ggttcgcccg
     1741 gtcacgggtc gcgcacgaac cagggagccg ttctcccgcc tcgagagcac ccaaaagagg
     1801 gtttcggcgg gcttcccttc gccggggggg accgtccgat atcttccccc cttgcgaccg
     1861 atcggttccc gggaaaccca ggaatcccgg gccggggtcc ctgaaaccca ggccgcatag
     1921 tccttgacca cccggcgatc ccggaacgca ttccccaagt gccgattcac ctcccgatcc
     1981 agggccagga gaaggatccc gctggtttcc cgatccaggc gatggtgcaa ggccacgtag
     2041 gggtctttcc cgcccagctt ttcccggtat cggtacgcgg cggcgaacac gttgttgtag
     2101 gcgtccgagg gcgtctgctg actgggaatt cccgcctcct tgtcgtaggc aagcaggcag
     2161 gaatcctgga acacgatacg ccggggatcg atctcgtagt gtcgacggac ccccgtccac
     2221 ggccagtaga cggcgatctc ttcccggccc tcgaggatgc gggcgggttt gcgttcccgg
     2281 tggccgttca cctggaccga cccgaagtcg atgaggtccg aagcctcttc cggggagacc
     2341 ttcaggcggc ttgccaggca ttgaaaaagg ggaacggaac gggaggcatc ctccgccctc
     2401 cagcgggtga ccgacagggg ggaacccggg ccgtgatcgg gtgcctgctt tctcatggaa
     2461 ggtcgtcttc cggggccgtc cccccggggt caagctcccc ccgggcttcg gcttccatgg
     2521 cttcctccac cagggcgtcc acgtcttctc ccaggtcctc tcccatctcc tgacccatgg
     2581 ttttcatcca ccgggccatg cttttgggat cgttttcatc caggcctccc aggcgcgacg
     2641 gatccgccat acgttccagg cgggtctcct cggaaaggcg caccctcacc cgggaaggga
     2701 ttcggcccac ctggcggctc ccgcagtgcc ggcagcaagg atccacctct tcggaaaccc
     2761 tcagggtgat gatttccgtc ttttttccac aatctccgca tcggaattca taaatcggca
     2821 taggcttttc atctcttcac gaaaatcgat gtgcttggtt tcaggattcc taagcttccc
     2881 gtcctgccgg gcggcccgca acacccggga tttagaacac ctcgagaaaa cagcacttga
     2941 gataccgggt ctcgggcatg gccaggagaa tggggtggtc tacggcctga gtccgggcct
     3001 cgaggaggcg gagccgtcgg ccgcttgcct gggcggcctg gagaaggatt tcctcaaaca
     3061 ggccttcgtc cacgtggtag gagcacgagc agctcaccag gattcccccg ggaggcagca
     3121 ggagcagcgc ccttcgattg agatccgtgt agcctttctt ggcggcggcg agggcgttgc
     3181 gggtcttggc gaaggaggga ggatccagaa tgatgcagtc ataatcggat ttgtttgcgg
     3241 atcgtaagaa atgaaaggct tcctcccgaa cgaagcggca gcgatcctcc agcccgttga
     3301 gagcggcatt ggccgcggcc tgctcgacgg cgtcccccga ctggtctacc cccgtcgcct
     3361 cgatggcccc gccggccact gcggcaagac tccacgcgcc gttatagcaa aacaggtcca
     3421 ggaccctgcg gcctgcggtc catcgttgaa aggcacgccg gttgtcccgc tggtcgagat
     3481 agaaccccgt cttttgtccc tcgaggggac gcagcgaata ctcgatcccg tcgatggaca
     3541 cccggcaatc ctcgggcagc cccccgtgga cgacgccctt ctccggggac aggccctcga
     3601 gggcccttac ggaagagtcg ttgcggcaaa ccagggccgc gggacggaag atctcgagga
     3661 gcaaatcccg gacccacggc tcgaggcgaa ccatccccag ggtgttgacc tggtaaacca
     3721 ccacgtcccc gtaacgatcc actacgaggc ccggcaagcc gtcggattcg ccaaatacgg
     3781 ctcggtaaca ggaggatccc gggtagtaaa attcctcccg tcgccgaacg gcctcccgga
     3841 ttcgctcttc caggaaatcc cgggagaagc ggactcccgg aggacaaagg atcctcaccg
     3901 caatcaggga ccgggggttg atgtagccgg agcccaggga gatgcctttg ccggaaaaca
     3961 cctccaccca gctccccggc tcgaactggg aaagagggtt cgtggaaatt tcgttgctga
     4021 aaacccatcg atgtccccga aggatcctct tttcccgtcc cgggtgaagg acgatgccgg
     4081 gcagttgagt gctgggcgat gcggactcca tgactggtgg cttctccccg gccggagctc
     4141 ccggccgtac cggcgggacc cggcgtcttt ccaacaaaaa aagcgcccca caagagggcg
     4201 ccggatgaaa acgggcccga gtcctaagac tcttcctcct tgcggaggga ctcgagcaaa
     4261 atctggtaat cctcgtacat gtctgcggac ccttcgatga tctcgtaaag gtcgtccagg
     4321 tccatgccga tgacggcatc cttgtccggc gattcttctt tcagctcgat ccagatccag
     4381 tccaggagca tttcctccgg ttcgtcgtag ccttcttcat tcttgtccag ggacccctcg
     4441 gccaccttct tgttcacctt tttcaaggct gccttcacaa agtctttcat ttccattcaa
     4501 acggcctccg tcgtattcga ccatgacccc caacaaggat gctgcttcag gtcatattta
     4561 tcgacagttg atgatctggt taagctcaat aagactctcg gattcgatcc tgttcgcagg
     4621 attctattca ttccggtgcc aaatggcaac ccaaacctgg attgtacctg attttaccgg
     4681 ccggagctct cattcaatgg gaccccccgg tggtgcctat tcgtgcccat aagaatagag
     4741 cgtcctgggg aaaagaattc cgaaaaaaac acgtccgaat agaaccggga gcgtgtaccg
     4801 cacaaggatt tccaatagaa acttgatttc ccgcgcttcc ctcccgtaag atggatcggg
     4861 ttgtccctgc tttttgacaa tacccttctt atcactcgcc ggcaccaaga tccattgaaa
     4921 aggaccgaca agatggccga aaaacaatat gtcattgacg ccatgaccgt ccaggattca
     4981 tggcggctct tcaagatcat cgccgagttc gtggatgggt tccagaccct ctcggacatc
     5041 taccccgcgg tttccatttt tgggtctgcg cgggtcaaac cgggagatcc ggtctacgag
     5101 aagacggtgg agatcgcccg caagctggcc cagaacgggt tccatatcct tacggggggc
     5161 gggccaggcg tgatggaggc gggaaacaag ggggcccggg aggggggagg caggtccgtg
     5221 ggcctcaaca tcaagcttcc cctggaacag gaacccaacc cgtatcagaa tgttcggctg
     5281 gattttcagt acttcttcgt tcgaaaggtc atgttcatca aatatgccca ggcctacgtc
     5341 gggatgccgg gggggttcgg caccatggac gagatcttcg aggccctgac cctcatacag
     5401 acccgcagga tcaagccgtt tcccgtcatt ctggtgggca aggaatattt tcacggcctc
     5461 ctggaatggg tgaagaacac cctgttgtcc cataatgcca tctcacgcga ggacctcgag
     5521 tttctcaccc ttctggatga tccggacgaa gtggtctata ccattaaaaa gtacgtgatc
     5581 gtctgatgag gaccggcgga cgagaaacga tccgggcacc ctcgagcctg ccgaacgccc
     5641 ttccggcatt ctttccgcgg aattggggcc gcggacggct tccccccctg cccatatgga
     5701 gccggacgcc gggggaatga cggccccctt cgatcccttc gtcgaccggg agcgtgtgtt
     5761 gcgtttagtg ggccccgtcc cgggacacaa actcgatctg cccttcgtgg ggaagcagga
     5821 tgggggatcc ctcgatccag atggaagtgc cgtaacgctc ttccaggcgc ttcaattcgt
     5881 ttcttttctg gttcaaaaga tagttggcca cctcgatgga gaaggtccct ctcacctggg
     5941 ccaggtcttt tc
//
LOCUS       DAOOVC010000201         5908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7749_length_5908_cov_8.123868, whole genome shotgun sequence.
ACCESSION   DAOOVC010000201 DAOOVC010000000
VERSION     DAOOVC010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7749_length_5908_cov_8.123868"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="ribD"
                     /locus_tag="PLM79_16640"
     CDS             <1..886
                     /gene="ribD"
                     /locus_tag="PLM79_16640"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOV87985.1"
                     /translation="NHFGRTPPCTGAILDAGIARVVVGMRDPNPHVAGGGIRALEERG
                     IVVECGVLEEECRRLNQPFIKHSTTGIPHVTLKAASTLDGRIASRTGDSKWITNERSR
                     RFVHRLRQSRDAVLVGIGTARADDPRLTVRLPGKTPRRQRVRIVLDTRLRLSPESYLV
                     RTAGEAPTWVACGQGAPSAAERALVERGVQVMRLPEEAGRVALVPLLRELGSRGVTSL
                     LVEGGAHVLGAFLDQGLADEFFFFYAPKILADGRGIPMIAGRARPSLREAVEVHDVRI
                     RRFGADWMVFGRFRANLY"
     gene            899..1561
                     /locus_tag="PLM79_16645"
     CDS             899..1561
                     /locus_tag="PLM79_16645"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOV87986.1"
                     /translation="MFTGLIEGTGLLLRMDRRGADAQMVLRASFPMERLALGESISVD
                     GACLTVASFQGDVFAADVSAETLRRTTLGRKTSGSRVNLERALRLGDRLGGHLVSGHV
                     DGIGVLKERRPEGRSLKLSFEIPPDLSVYVVEKGSIAVNGISLTVNACSPGRFEVNII
                     PHTSGETTLGEIRVGEEVNIETDLIGKYVAKMLDSWLNSSRKTEKPGRIDPSFLQKHG
                     FL"
     gene            1741..2979
                     /locus_tag="PLM79_16650"
     CDS             1741..2979
                     /locus_tag="PLM79_16650"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOV87987.1"
                     /translation="MPLATIPEALEDIRQGKMVILVDDEDRENEGDLCMAAEKATPEA
                     INFMARYGRGLICLSLTPEWVDRLRLPMMVSNNLSRFQTAFTVSIEARQGVTTGISAA
                     DRAHTILTAIADNAKPEDLVSPGHVFPLRAKKGGVLVRTGQTEGSVDLARLAGLKQAG
                     VICEVMKDDGTMARMPDLERFSQEHGVKIVAVADIIRYRMQHELFVHKSAEAHIPSAW
                     GGEFRVIAYTNDIDEYTHLALVKGEIDPEEETLVRVHSECITGDVFGSLRCDCGGQLR
                     AALSRIGKEGRGVILYMRNHEGRGIGLLNKIKAYALQEKGYDTVEANQVLGFQPDLRD
                     YGIGAQMLVDLGVRKMRLMTNNPKKVVGLEGYGLKITERVPLEIPPNESNIRYLSTKC
                     TKMGHLLSCVLQEDVHHESL"
     gene            2966..3430
                     /gene="ribE"
                     /locus_tag="PLM79_16655"
     CDS             2966..3430
                     /gene="ribE"
                     /locus_tag="PLM79_16655"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOV87988.1"
                     /translation="MKVYEGKLLAQNLRFGIVVSRFNDFICDRLLGGALDALQRSGAD
                     EKAIDVYKVPGAFEIPLIARKVASTGRYDAVICLGAVIRGATPHFDYVANEVSKGVAH
                     VGLETGVPIAFGVLTTDTLEQAIERAGSKPGNKGWDAAMAAVEMANLIQQIA"
     gene            3437..3877
                     /gene="nusB"
                     /locus_tag="PLM79_16660"
     CDS             3437..3877
                     /gene="nusB"
                     /locus_tag="PLM79_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOV87989.1"
                     /translation="MGRRRRSREIALQILYEMEMAQVPPDQAISLVYEHFEAPSSSRP
                     FTENLVMGVVLHRKEIDQLIAAASEHWRIDRMSIVDRNILRIALFEMLHCEDIPPKVS
                     INEAIDLGKTFGTEDSGAFINGILDHMLLELKESRRIPGDRSPS"
     gene            complement(3894..4151)
                     /locus_tag="PLM79_16665"
     CDS             complement(3894..4151)
                     /locus_tag="PLM79_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87990.1"
                     /translation="MMPRLGEKYKDIEIEVVSKPIAEYQCDEYFELELPVAPAIMVGD
                     EIVVEGSDISQDKLETALCRHLGLPEPEPSKRGLFKRIFGA"
     gene            complement(4232..5584)
                     /locus_tag="PLM79_16670"
     CDS             complement(4232..5584)
                     /locus_tag="PLM79_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOV87991.1"
                     /translation="MAVKLGYRNVYRDPYGYPQWQARGMPVESAPAGLAQTREEPGLP
                     GPLYGWGMLWTLLGIFAGGMALNLTPCVYPLIPITVSYFGGQAAKGGAGRGKLLVHGL
                     NYMTGLALTNSILGVVASLTGSLMGSILQNPIVLLVVASVLVLFATSLFGFWEMRLPS
                     GLTQAAAKSYTGYLGSLFMGLTLGVVAAPCIGPFVLGLLTWVAGMGSPLLGFAVFFTL
                     SLGLGLPLFFLAVFSGQLEKLPRSGDWMIWVRKLMGWVLVAMAVHFIRPVLPGRWSSL
                     PIALVALAAGLHLGWMDQSEARFKAFPWLKGGAGVTWLVLCAFLVTSWTLQGPGIAWK
                     PYSEEVLKEAQYLKKPVILDFYAGWCTPCRELEEVTFHDASVVKEADGRFVMVKVDLT
                     KGGDPVHERLLQQYEIKGVPTIVFLDPSGKERRDLRLVDFLPADQFLGRMAQLNKGGS
                     "
     gene            complement(5624..>5908)
                     /locus_tag="PLM79_16675"
     CDS             complement(5624..>5908)
                     /locus_tag="PLM79_16675"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOV87992.1"
                     /translation="RAVAPKQASWEDVLAQGKAGGYRIITTEELSERYGTDPKGFLLV
                     DTRQEWEYRTGHLQGAVNFPMEPTSWARWRKASALETLLGPDKDRTVIFY"
BASE COUNT         1213 a         1789 c         1817 g         1089 t
ORIGIN      
        1 caaccatttc ggacggaccc ctccctgcac cggcgccatt ctcgatgcgg ggatagcccg
       61 ggtggtggtg gggatgcggg acccgaatcc ccacgtggcg ggagggggaa ttcgagccct
      121 cgaggaacgc ggaatcgtcg tggaatgcgg ggtcctcgag gaggaatgcc gccgcctgaa
      181 ccagcccttc atcaagcatt ccaccacggg aatcccccac gtgaccctca aggccgcctc
      241 gaccctggac gggcgcattg cgtcgcgaac gggcgattcc aagtggatca ccaacgaacg
      301 atcccggcga ttcgtccatc gcctccgcca atcccgggac gccgtcctgg tggggatcgg
      361 aaccgcccgg gcggacgacc ctcgactgac cgtgcggctt ccgggaaaga ccccccgacg
      421 gcaaagggtc cgaatcgtgc tggacactcg attgagactg tctccggagt cctacctggt
      481 ccggaccgcc ggggaggctc ccacctgggt ggcgtgcggg cagggagccc cctcggccgc
      541 agagcgggcc cttgtggagc ggggggtgca ggtgatgcgg ttgccggaag aggcgggccg
      601 ggtggccctg gttcctctcc tccgggagct gggaagccgc ggggtgacca gcctgctggt
      661 ggaagggggc gcacacgttt tgggggcctt cctcgaccag ggtcttgccg atgagttctt
      721 cttcttttac gcgcccaaga tcctggcgga cggccgggga attcccatga tcgcgggaag
      781 agctcgaccg agcctccggg aagccgtgga ggttcacgac gtccgcatca ggagattcgg
      841 cgcggattgg atggtgttcg ggcggttccg tgcgaacctc tattgaaccg gtgtgccgat
      901 gtttacggga ctgattgaag gaacgggtct gctgctgcgg atggatcgtc ggggggccga
      961 tgcgcagatg gtgcttcggg cctcctttcc catggaacgg cttgccctgg gggagagcat
     1021 ttccgtggac ggggcctgtc tcaccgtggc ctcttttcaa ggagacgtct ttgccgcgga
     1081 cgtttctgcg gaaaccctcc ggaggaccac cctgggccgc aagacgtcgg gttctcgagt
     1141 caacctggaa agggcgctcc ggctggggga ccggttgggc gggcacctgg tgagcggcca
     1201 cgtggacggg atcggggttt tgaaggagcg ccgacccgaa ggacgttccc tgaaactctc
     1261 cttcgagatt ccgccggatc tttccgtcta cgtggtggaa aagggctcca tcgccgtcaa
     1321 cggaatcagt ctcacggtga acgcgtgcag cccgggacgc ttcgaggtga acatcattcc
     1381 gcacacctcc ggggaaacca ccctcgggga gatccgggtc ggcgaagagg tcaacatcga
     1441 gacggacctc atcggaaaat atgtggccaa gatgctcgat agttggctga acagttctcg
     1501 gaaaacggaa aaacccggtc gaatcgaccc gtcttttctc caaaagcacg ggtttctgta
     1561 ggggaaaccc gggagggaaa gccgattcca cggggggaca tttccccgga gccttccccg
     1621 gatcgtcggc ttgagatgtg gaattcttcg tgttatagat ccatggatgt aagggcgagg
     1681 cccggcgagg cctccaccgc ttcccttgca caatccctca accaaaggag ctcgatcaag
     1741 atgcctctgg caacgatccc ggaagccctg gaagatattc ggcagggcaa gatggtgata
     1801 ttggtggacg atgaagaccg cgagaacgag ggggacttgt gcatggccgc ggagaaggcc
     1861 acccccgagg cgatcaattt catggcccgt tacggtcgag gtctcatctg cctgtctctc
     1921 acccccgagt gggtggatcg cctgcgcctt cccatgatgg tctccaacaa cctgtcccgc
     1981 ttccaaacgg ccttcaccgt ttccatcgag gcccgccagg gggtgaccac ggggatcagc
     2041 gccgccgacc gggcgcacac cattcttacg gccattgccg ataatgccaa acccgaagac
     2101 ctggtgagcc cgggccacgt atttcccctt cgcgccaaga aagggggagt cctggtaagg
     2161 acgggccaga cggaagggtc ggtggacctg gcccgcctgg cgggactgaa acaggccggg
     2221 gtcatctgcg aagtcatgaa ggacgacggc accatggccc ggatgccgga cctcgagagg
     2281 ttctcccaag agcacggagt caaaatcgtg gccgtagcgg acatcattcg ctaccggatg
     2341 cagcatgagc tcttcgtcca caagagcgcc gaggctcaca tcccctccgc ctggggaggg
     2401 gaatttcggg tcattgcgta caccaacgac atcgacgaat acacccacct cgccctggtc
     2461 aagggggaaa tcgaccccga agaggaaacc ctggtgaggg tgcattccga atgcatcacc
     2521 ggggacgtct tcgggtccct gcgctgtgac tgcggaggac agctgcgggc cgctctgagc
     2581 cgcatcggca aggagggccg cggggtcatc ctgtacatgc gcaatcacga aggccgcgga
     2641 atcggtcttc tcaacaagat caaggcctat gccctgcagg aaaagggata cgacacggtg
     2701 gaagccaacc aggtcctggg gttccagccg gacctgcggg actacgggat cggggcccag
     2761 atgctggtgg acctgggagt gcgcaaaatg cgcctcatga ccaacaaccc caagaaggtg
     2821 gtgggtctcg agggctacgg gctgaagatc accgaacgag tgccgttgga aattcctccc
     2881 aacgagtcca atatccggta cctgagcacc aagtgcacca agatggggca tctgctgagc
     2941 tgcgtcctgc aggaggatgt gcaccatgaa agtctatgaa ggcaagcttc ttgcccagaa
     3001 cctccgtttc ggtatcgtgg tgagccgctt caacgacttc atctgcgatc ggctgttggg
     3061 gggggccctg gatgccctgc agcgaagcgg tgccgatgaa aaggccattg acgtttacaa
     3121 ggtgccgggg gccttcgaga ttcctctcat cgcccggaaa gtggcctcca ccggtcgcta
     3181 tgacgcggtc atctgcctgg gtgcggtgat tcggggagcg acccctcact tcgattacgt
     3241 ggccaatgaa gtctccaaag gggtggccca cgtggggctc gagaccgggg tgcccatcgc
     3301 cttcggagtc ctgaccaccg ataccctgga acaggccatc gaacgggccg ggtccaagcc
     3361 gggaaacaag ggttgggatg ccgccatggc ggccgtggag atggccaacc tcatccaaca
     3421 gatcgcatga tgcctcatgg gccgacgcag acgttcccgg gaaatcgctc tacagatcct
     3481 ctatgaaatg gaaatggctc aggtccctcc ggaccaggcc atttccctgg tctacgaaca
     3541 tttcgaggct ccgtcctctt cccgtccctt cacggaaaac ctggtgatgg gagtcgtcct
     3601 ccaccgcaag gaaatcgacc agcttatcgc ggccgcctcc gaacactggc gcattgaccg
     3661 catgtccatc gtggatcgaa acatcctgcg aatcgccctg ttcgaaatgc ttcactgcga
     3721 ggacatccct cccaaggtct ccatcaacga agccatcgac ctgggaaaga ccttcggcac
     3781 cgaggattcc ggcgccttca tcaacggaat cctggatcac atgctcctgg aactgaagga
     3841 aagccggaga atcccggggg accggtctcc atcctgacgt cgttcacggt tcctcatgcc
     3901 ccgaaaatcc gcttgaagag gcccctcttc gaaggttcgg gctcgggaag ccccaagtgc
     3961 cgacagaggg cggtctccaa cttgtcctgg gagatgtcgg atccctccac cacgatctca
     4021 tctcccacca tgatggccgg cgcaacggga agctccagtt cgaagtactc atcgcattgg
     4081 tattccgcga tgggctttga aacgacttcg atttcgatat ccttgtactt ttcacccaac
     4141 ctgggcatca tccggagaaa attctttcag ggcttgccgt tgggctcatt caagaaaaac
     4201 ctgaccttga gcatctttgt ttctcctttc cctagcttcc gcctttattg agctgtgcca
     4261 tccgccccag aaactggtcc gccggaagga aatccacgag gcgcaggtca cgcctttcct
     4321 tgccgctcgg gtcgaggaac acgatcgttg gaaccccttt gatctcgtat tgctgaagca
     4381 gccgttcgtg gacgggatcg cctcccttgg tcaagtccac tttcaccata acaaaacggc
     4441 cgtctgcctc cttcacaacg gaagcatcgt ggaaggtgac ttcctcaagt tcccggcacg
     4501 gcgtgcacca gccggcatag aagtcaagga tgacgggctt tttcaagtat tgagcctctt
     4561 tcaatacctc ctccgagtag ggcttccagg caatccccgg tccctgaagg gtccatgagg
     4621 tgaccaggaa agcgcagaga accagccacg tgaccccggc tccgcctttc agccacggga
     4681 aagctttgaa tcttgcctcg ctttggtcca tccacccgag atgcaacccg gcagccaggg
     4741 ctacgagggc aatcggcagg gagctccacc ggccggggag aaccggccga atgaaatgca
     4801 ccgccattgc gaccaggacc cagcccatga gcttccttac ccagatcatc cagtcgcccg
     4861 agcggggaag cttttccagt tgtcccgaaa acaccgccag gaaaaacagg ggcaatccca
     4921 ggcccagact cagcgtaaaa aagacggcaa atccaagcaa cgggcttccc atgcccgcaa
     4981 cccaggtgag gagtccaaga acgaagggac cgatgcaggg agctgcaacc acgcccaggg
     5041 tgagtcccat aaagaggctt ccaaggtagc ccgtgtagga ttttgcagca gcctgggtga
     5101 ggccgctggg aaggcgcatc tcccagaacc cgaaaaggct cgtggcaaac aagacaagca
     5161 ccgaggcaac gacaaggagg acgatggggt tttggaggat cgatcccatg aggctgccgg
     5221 taagggacgc aaccactccg aggatggagt tggtgagggc aaggcccgtc atatagttca
     5281 aaccatgcac gaggagcttt ccccggcccg ctcctccctt ggcggcttga ccgccaaagt
     5341 aggaaaccgt gatggggatc aaggggtaga cgcacggagt gaggtttaaa gccatgccgc
     5401 ccgcaaagat gccaaggagg gtccatagca tcccccagcc atatagcggc ccggggagcc
     5461 cgggttcttc cctcgtttga gcaagacccg ccggggcgct ctcaacgggc atgcctcttg
     5521 cctgccactg gggataaccg tacggatctc ggtagacgtt cctgtagcca agctttacgg
     5581 ccacacgggc cgccgagtcg ctgcgtactc acgtgaggcc cgctcagtag aagatgacgg
     5641 tgcgatcctt gtcgggtccc aggagggttt cgagagccga tgccttgcgc catctcgccc
     5701 atgaggtcgg ctccatgggg aagttcaccg ctccttgaag atgccctgtg cggtattccc
     5761 attcctggcg ggtgtctacc agaagaaagc ccttgggatc cgtcccgtat cgttcggaaa
     5821 gctcttcggt ggtgataatc cggtagcccc ccgctttccc ttgggccagg acatcctccc
     5881 atgaggcctg cttgggagca accgcgcg
//
LOCUS       DAOOVC010000202         5859 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7801_length_5859_cov_3.846657, whole genome shotgun sequence.
ACCESSION   DAOOVC010000202 DAOOVC010000000
VERSION     DAOOVC010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7801_length_5859_cov_3.846657"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PLM79_16680"
     CDS             complement(<1..1070)
                     /locus_tag="PLM79_16680"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOV87993.1"
                     /translation="MERSREETYRLIGKAGLIILLLTLGDKVLAVVKEMVMAGRFGIA
                     PSLDAFNIAYAYPAIFVLLASGALVSAFVPLYIEWCQVHSGPEADARARALLLLTTGS
                     FGLIAVGSSFLSPYIFPLFGHGFEAGDKKLGIALQRLLVFLIFLDGTAILLNGLLLAK
                     KRFAALHTAPMFINASIIAFLVFQPQLGIYTLVWGFLAGTLCKLLYLGVTLRISGFRF
                     WARPRFDGVAVRVFFSLAFPLLGSELISNMNILVDQVMATNLSSGSVSTLRYAYRLND
                     LPIQLVVLGLSKAIFPFISESALQNDIEGLRDVYGRCLVFLAFLTLPVVCLVSLFSRE
                     IVSLLLQRGAFDENAARLTADTL"
     gene            complement(1077..2507)
                     /locus_tag="PLM79_16685"
     CDS             complement(1077..2507)
                     /locus_tag="PLM79_16685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOV87994.1"
                     /translation="MLRGDVFPVKVHKAADVVLTGATFVGAYLIKRYLLPEPWGGLTD
                     IPNYTGILLLVVVLWYMSFEMTGLHAAYRRLSFRQSVSRLIQLVTVNLVLTFLFFYLL
                     KIENVSRILMGIFYGLNLATLAWSAWYAREIHRKKGFNLLVVIIGSREAAKEFIEQIV
                     RDPDANFRVLGCLEVSPEEVGKSVTEGIEVIGTLGELQDVLTRHVVDEIIFAMPIDKI
                     WEAERYMAVAEAMGVQIRILPHWHLRKFLMTRPSYYQMYFEEFLASPSLVLTATSRKR
                     LALLVKGVMDYAGAAFGLLIMVPVGLLLALFIFAVSPGPVFYKQVRCGVHGRRFTLYK
                     FRTMVPNAEKLLPNLAHLNEASGPVFKMKDDPRILPFIGKFMRRTGLDEMPQLLNVLR
                     GEMSLVGPRPPTPAEVACYSLQERRRLSMKPGLTCIWQVTPGRNRITFQEWMAMDLAY
                     IDHWSLWLDIRILALTTRAVLFGYGQ"
     gene            complement(2736..3893)
                     /locus_tag="PLM79_16690"
     CDS             complement(2736..3893)
                     /locus_tag="PLM79_16690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV87995.1"
                     /translation="MASISVTGLRGIPATWGGVEHQCEQLYSRLAARGHAVVVYARSG
                     YIPPGTRRHKGIRIVRLPTVRTKHGEAFVHTFLSVLHILKENPRIVHFYSQGPALFAP
                     LVRLLRPRTKVFFTCGGLDWRRKKWEPWASKVLHLGEWCSARFPHYRIMVSEELKRYY
                     EARYGVKAHCILNGVERGTKRAPGFIRSFGLEGRDYALFVGRLVPEKRIEDLITPFLD
                     GSFRSKLVIVGDSAGTEDYVDGLKALASGAPNVLFLGYRYGEVLEELYSNARCFLTAS
                     ELEGLPLTLLEALSFGLVCVCSDIPPHREVVSKTGGRLFPVGDRAALARSIQEMERMT
                     AQELLSFESHVVETVEKHFSWDEAARRLERLYRESLRGEAAGSRMPVCRNR"
     gene            complement(3918..5153)
                     /locus_tag="PLM79_16695"
     CDS             complement(3918..5153)
                     /locus_tag="PLM79_16695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV87996.1"
                     /translation="MGKRRLTTKEQPLSKKMRILHVIDALNHGGAQRLLMLLAEKAPR
                     DRVETGVLVLQPRLELKEALESLGVRVRCCNRERPSILSPHRFLSYVLRNLREIAAFC
                     RSLSADVLHCHLSDAELLGIPSGRLCGVSRVFTTVHYPDLLPKRKPADLRNALRRALT
                     RILYNHLADGVVTVSRDTAEILSRDFGVRPEKIQVILNGVDVASFSPRTPPPDARRSL
                     GFNQKDCILINVARLAPPKGQAFLVEAMARLTAQFPGIRLLFVGDGELRAELEARRAS
                     LRLEDTVVFLGNRSDIADLLALSDIFVFSSVSEGTSLALLEAMAAGKPIVATSIPGNE
                     EVLRHGENAYLVPPGDPDALARGVAFLLDHPDEAQRLAAKGQQEVAAQFDIGQTIARY
                     RALWTGLDTDTVLGAKRTA"
     gene            complement(5218..>5859)
                     /locus_tag="PLM79_16700"
     CDS             complement(5218..>5859)
                     /locus_tag="PLM79_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV87997.1"
                     /translation="MKSMRSSLENIWATILLHAPERPGNLLLCGSTPGEGVTFLSFHL
                     ALFLYLEYNLKVLYVDTDPGGKRPYLQGSSKQSFPGLGAYFNQGRPLDSLILKTEFPG
                     FFILPGGDLENMAKTSTIIGEKESVEALMDYTATHFHAAIFNGTPVLRSPGILGLAKA
                     VDQVVLVCRYKVSRREVSSLALAKLRECGAPVLGVILNDRRYPIPSRVYKILK"
BASE COUNT         1256 a         1713 c         1784 g         1106 t
ORIGIN      
        1 agggtgtccg ccgtcaggcg ggccgcattc tcgtcgaagg ccccccgttg cagcaaaagg
       61 ctcacgatct cgcgggaaaa gaggcttacc aggcagacga cggggagagt caggaaggcc
      121 aggaacacca ggcatcggcc gtagacgtct cggagtccct cgatatcgtt ttgcagggca
      181 ctctcgctga taaaggggaa aatggctttg gacaatccca ataccaccag ttggatgggc
      241 agatcgttca accgataggc gtaccggagg gtggaaacgg acccggagga aagattcgtg
      301 gccatgacct ggtccaccag gatattcatg ttggagatca actcgctgcc caggagcggg
      361 aaggccaggg aaaaaaagac ccggacggcc accccgtcga accggggccg ggcccaaaaa
      421 cgaaacccgg aaattcgaag ggtgactccc aggtagagca acttgcagag ggttcccgcc
      481 aggaatcccc agaccagggt gtagattccc aactggggct ggaagaccag gaaggcgatg
      541 atggaggcat tgatgaacat gggggccgtg tgcagggcgg caaatctctt tttggcgagg
      601 agcaacccgt tgagcaaaat ggcggttccg tccaggaaga tgagaaacac cagcaggcgt
      661 tgcagggcga tgcccagctt tttgtccccg gcttcaaaac cgtggccgaa gagagggaag
      721 atgtaggggc tcaggaagga gctgcccacg gcgataagac caaaggaccc ggtggtgagc
      781 aggaggaggg cccgtgccct tgcgtccgct tcgggacccg agtgaacttg acaccattcg
      841 atgtaaaggg gtacgaaggc ggataccagg gctcccgagg caaggagaac aaagatggcg
      901 gggtaggcgt aggcgatgtt gaaggcgtcc agggagggcg cgatgccaaa ccggcccgcc
      961 atcaccattt ccttgaccac cgccaggacc ttgtccccca gggtcaggag gagaatgatg
     1021 aggcctgcct tgccgatgag ccgataggtc tcctccctgg aacgctccat cggtcctcac
     1081 tgcccgtatc caaagagaac ggcccgggtg gtcagagcaa gaatcctgat gtcgagccac
     1141 agggaccaat ggtcgatgta ggccaggtcc atggccatcc attcctggaa cgtaatccgg
     1201 ttccttccag gggtaacctg ccagatgcat gtcagccccg gtttcatgga cagccgcctt
     1261 ctctcctgca gactgtagca ggcgacctcc gccggggtgg gcggccgggg acccacgaga
     1321 ctcatttctc cccgcagcac attgagcagc tggggcattt cgtccagccc ggtccgcctc
     1381 atgaacttgc cgatgaaggg aagaatcctg ggatcgtcct tcattttgaa caccggaccg
     1441 ctggcctcgt tgaggtgtgc caggttcgga agcaacttct cggcgttggg aaccatggtt
     1501 cgaaacttgt acagggtaaa tcttcttccg tgcaccccgc atcggacttg cttgtagaag
     1561 acgggaccgg gagagacggc aaaaataaac agggccagca ggagacctac gggaaccatg
     1621 atcagtagtc cgaaggccgc accggcgtaa tccatcactc ctttgaccag gagagccaga
     1681 cgcttgcggg aggtggccgt cagcaccagg ctggggcttg ccaggaattc ctcgaagtac
     1741 atctggtagt aggaaggccg ggtcatgagg aactttctca ggtgccagtg aggcaaaatc
     1801 cggatctgca cacccatggc ctcggcaacg gccatgtaac gctccgcttc ccagatcttg
     1861 tcgatgggca tggcgaaaat gatttcgtcc accacgtgcc gggtaaggac gtcctggagt
     1921 tccccgaggg ttccaatgac ctcgatgccc tccgtgacgg actttcccac ctcctcgggg
     1981 ctgacctcga ggcatcccag gactcgaaaa ttggcgtccg gatctcgaac aatttgctcg
     2041 atgaactcct tggcggcctc cctgctgccg atgatcacca ccagcaggtt gaaccccttc
     2101 ttccgatgga tctcccgggc gtaccaggca ctccaggcca gggttgcaag gttcaatccg
     2161 tagaagattc ccatgagaat ccggctcacg ttctcgatct ttaagaggta gaagaagagg
     2221 aacgtgagta ccaggttgac ggtcacgagt tgaatgagtc tcgagacgct ctgccggaag
     2281 ctgagtcgtc ggtaggccgc gtgcaggccg gtcatctcga agctcatgta ccagagaacg
     2341 accacaagaa gaagaatccc ggtgtaatta gggatgtcgg tgagtccgcc ccacggttcc
     2401 ggcaggaggt acctcttgat aagatacgcc ccaacgaagg tggctcctgt gagtacgacg
     2461 tccgccgcct tgtggacctt tacgggaaaa acatcccccc tgagcattcc cttgctccca
     2521 caaccttaag ttgctctgct gtgcctcttc ccgaccgcgc cgccctctcc ctcggcggcg
     2581 aagtcggccc atctgtgccg acgacgtgga cacacacccc gattccttgc ccgcacaacg
     2641 ttatgaaggg ggaatgaagg ggacccggac cgccaaggac ccgatcctcc atcatcccgg
     2701 ccttctctcc ataacgccgg ggagaatccg ctctcctacc ggtttctaca caccggcatg
     2761 cgggacccgg cagcctctcc ccggagactc tcccggtaca ggcgctccag gcgtctcgca
     2821 gcttcgtccc agctgaagtg cttctccacg gtctcaacga cgtgtgattc aaaactgagc
     2881 aattcttgtg ccgtcattct ttccatttcc tgaatacttc ttgcaagggc ggcccggtcc
     2941 cccacgggga ataagcgccc ccccgtcttg ctcaccactt cccgatgggg gggaatatcg
     3001 ctgcacacac agaccaaccc gaaagacagg gcctcgagaa gagtcagggg caggccttcc
     3061 agttccgacg ccgtcaggaa acaccgggcg ttggagtaga gttcctccag gacctctccg
     3121 taccggtatc ccagaaagag gacgttgggc gcccccgaag ccaaggcctt gagcccgtcg
     3181 acataatctt ccgtacccgc gctgtcgccg acgatgacca gcttggaacg gaaggaaccg
     3241 tcgagaaatg gggtgatgag gtcttcgatg cgcttttccg gcaccaggcg acccacaaaa
     3301 agagcgtagt cccgcccttc cagtccgaaa gagcggatga agccgggagc cctcttggtt
     3361 cctctctcca ccccgttcaa gatgcagtgg gcctttaccc cgtaccttgc ctcgtagtag
     3421 cgcttgagct cttccgatac catgatccgg taatgtggaa agcgggcgga acaccattct
     3481 cccaggtgga ggactttgga tgcccagggt tcccacttct tccttcgcca gtccagtccc
     3541 ccgcaggtga aaaagacttt cgttcgaggg cgcaggagcc tcaccagggg ggcaaacagg
     3601 gcgggaccct gggaatagaa atgcacgatc cggggattct ccttaagaat gtggaggacc
     3661 gacaggaagg tgtgaacaaa cgcttcccca tgcttggtcc tcaccgtggg caggcggaca
     3721 atgcgaatcc ctttgtgccg gcgggtgccg ggaggaatgt agccgcttcg agcgtacacg
     3781 acgacggcgt ggccccgggc ggcgagcctc gagtagagct gttcgcactg gtgctcgacc
     3841 cccccccaag tggcgggaat cccccgcagt cctgtcactg aaatggaggc catggccgtg
     3901 tccaactcct cgaaaggtca ggcggttcgt tttgcgccca ggacggtgtc cgtgtccagc
     3961 ccggtccata gcgcccggta tcgggcaatg gtttgcccga tatcgaactg tgcggcgact
     4021 tcctgttgtc cctttgccgc aagtctttgt gcctcgtcgg gatgatccag gagaaaggca
     4081 actccccgcg ccagggcgtc gggatctccc ggtgggacca agtacgcgtt ttcgccgtgg
     4141 cgaaggactt cttcattgcc gggaatggag gtggccacga tcggttttcc cgccgccatg
     4201 gcttccagga gggccaggga agtcccctcg gaaaccgagg aaaagacaaa gatgtcactc
     4261 aaggcgagaa ggtccgcgat gtccgaccgg ttgcccagaa aaaccaccgt atcttccaaa
     4321 cggaggcttg cccgccgggc ctccaactcc gccctcagtt ccccatcgcc gacgaaaagg
     4381 aggcggattc ccgggaattg ggctgtcagc cgagccatgg cttccaccag gaatgcctgg
     4441 cccttgggcg gagccagtcg agccacgttg atgaggatgc aatctttctg attgaagccc
     4501 aggcttctgc gggcatccgg gggcggggtc ctcggggaaa aggaggcaac gtccaccccg
     4561 ttgagaatga cctggatctt ttccggtcgg acaccgaaat cacgagaaag gatttccgcc
     4621 gtgtcccggg aaacggtcac gactccgtcc gccaggtgat tgtagagaat ccgggtcaag
     4681 gcccttcgaa gggcgtttcg aagatcggcg ggcttgcgtt tggggagaag gtccgggtag
     4741 tgcaccgtgg tgaagactct cgagaccccg caaagccgtc ccgagggaat gcccaggagt
     4801 tcggcgtccg ataagtggca gtgaagcacg tccgcagaca gggacctaca gaatgccgcg
     4861 atctcccgga ggtttcggag cacgtaggaa agaaagcgat gggggctcag gatggaggga
     4921 cgttcccggt tacagcagcg aactcggact cccagggatt ccagggcttc tttgagctcg
     4981 agccggggtt gcaggaccag cacgccggtt tccacccggt ctcgaggtgc cttttccgcc
     5041 aggagcatca ggagcctctg ggcgccgccg tggttgagag cgtcaatgac atgcaggatc
     5101 ctcatttttt tggacaaagg ctgttccttg gtcgtcaacc gtcgttttcc cattctccta
     5161 ctgggaacgc ggtccctctc gaatcgggtt cgaaccggag ggggtgtccc gggcacccta
     5221 cttcagaatc ttgtagactc gagaaggaat gggatatcgg cggtcgttga gaatgacccc
     5281 caggaccggg gccccgcatt ctcgaagctt tgcgagagcc agtgagctca cttcacgtcg
     5341 agataccttg tatctgcaga ccagaaccac ctggtccacg gccttggcca atccgaggat
     5401 cccaggagaa cggagaaccg gcgtgccgtt gaagatggcc gcgtggaagt gggttgccgt
     5461 ataatccatg agcgcctcga cactttcctt ctctccaatg atggtgctgg tcttggccat
     5521 gttttccagg tccccgccgg gcaaaatgaa gaacccggga aactcggtct tcaggatgag
     5581 ggaatccagg gggcggccct ggttgaagta ggctcccagc ccgggaaagc tttgcttgct
     5641 cgacccctga agatagggcc tcttccctcc gggatccgta tccacgtata ggactttgag
     5701 gttgtattcg aggtacagga acagggcgag gtgaaaggac aaaaaggtaa ccccttcgcc
     5761 gggggtggaa ccgcacaata gaaggtttcc gggtctctcc ggcgcatgga gcaggatcgt
     5821 ggcccaaata ttctcgaggg aggatcgcat cgacttcat
//
LOCUS       DAOOVC010000203         5858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7802_length_5858_cov_5.997415, whole genome shotgun sequence.
ACCESSION   DAOOVC010000203 DAOOVC010000000
VERSION     DAOOVC010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7802_length_5858_cov_5.997415"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(458..2440)
                     /locus_tag="PLM79_16705"
     CDS             complement(458..2440)
                     /locus_tag="PLM79_16705"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOV87998.1"
                     /translation="MPRPSPSSTSDSRRGRITVFWIVLSFLGSHLLFFLFPRVLPSWN
                     EQVFDRFFVLRSSLPSFQPPYDGTVVHVDVTNSTIRRLGNFYLNRSHYARVMENLGTM
                     GTFAQAYDFIFPARTNETEDEAFLSAARKSANAYFGMAFVLDAVEGPGGSSVQKTAGI
                     ESLEAGRWKLRVEGDASGLYRGSDPLTTFSELAERSRGVGFLNVTADPDGVFRRVPLV
                     VRFQGGFYPSFPFRVACDYLGVPPERIVLRPGKHVLLENARHPLWEKPRDLRIPVDAR
                     GNLRINYAGPWERMKHYNFAAVYRASEDREELALWKEELSGKIVIVSDVATGSADQGP
                     VPTDANLPLSGLHANVLNTILSGAFLQELSFLQGLLLDGALAGCVLVLAALASSYGFP
                     LLCLALAAGVSGLALALFLYAHTLIPLLRPLTAILLSAGSITAVRYIRVSREKAVLRR
                     LFEAYFPPSVVKKLVARPDVLASRGQRKELSILFSDIQGFTTHSARLTPEQIQKSLNE
                     YFDSMVEIVFRHEGTVDKYIGDGLMVFFGDPDPQEDHALRAVRAARDMQKKVRELDAA
                     WARDGGMPLKIRIGINTGEVVVGNMGSKKRLSYTVLGSAVNLAQRLESNAPVGGILVS
                     RRTYELVRDHVPSRSLGEIRVKGLETPVPVYEIPVS"
     gene            complement(2409..3119)
                     /locus_tag="PLM79_16710"
     CDS             complement(2409..3119)
                     /locus_tag="PLM79_16710"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HOV87999.1"
                     /translation="MRRIQTRGTLWGTLIPVFLILASLFPLLSPSFAHSQDQTVTFRW
                     AFGTLIDEGLTLRLEPIREDRSMKSGDHFKMMVEPRTRCFVYVIYHNSQGEMIRLFPY
                     SLEQFPAGFELNKRYYIPQGERWFELDTHPGRETFYLLASARRLVELETLLKTYESAD
                     PEKKPDLTTQILAQIRTERLLHREFAESAERPVAIGGAIRGIEKAQGENRQSIDPIAE
                     VISSTTGFYAKTFTIEHQ"
     gene            complement(3234..4898)
                     /locus_tag="PLM79_16715"
     CDS             complement(3234..4898)
                     /locus_tag="PLM79_16715"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOV88000.1"
                     /translation="MNRVVGMIGLLAFCALSWAPNPAWGMEKTGRPPSSPAAVPGGEQ
                     RTALVIGNGSYETSPLRNPVNDARAIARALAEVGFEVSERENLSQKDMKREIQAFGQK
                     LQKGGVGLFYYAGHGMQVDGKNYLIPIGAKIEHEKHVEYEGVDVGSVLSEMEYARNRL
                     NIVILDACRDNPFARSFRSGAQGLASVNAPIGTIIAYATAPGSVANDGPEDHGVYTGE
                     LIRVMKAPGVKIEDVFKQVRSAVREGTQGRQVPWESSSLEGDFFFMPGSVAAAPVSVA
                     ENKPTVVASSTSRGEASPKAVKSWKEPVSGMEFLWIPGGCFNMGSPETEAGRDADEGP
                     VHEVCLEGFWMAKTETTNGQFRKFQPNHDSRDYDGFSLNGDNQPAVHVTWEDANGFAQ
                     WLKAQNGGKYKFRLPTEAEWEYACRAGSDAARYWGDDPNRACTFENVADHTARNQWGW
                     EESHDCDDGYAATSPVAAFQPNAFGLYDMLGNVWEWCEDTYGIDVYTRHDRNGPVYVN
                     AGVGADRVIRGGYWHGSPRSVRCADRGSGLPNGMNDDLGFRLVREP"
     gene            5474..>5858
                     /locus_tag="PLM79_16720"
     CDS             5474..>5858
                     /locus_tag="PLM79_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88001.1"
                     /translation="MKPGDVFSVKDPEMLRGMEAVCRRYPFRAGRYVCGLVRHPGDPV
                     WKQIVPDPAELEDEDGFEDPLAEESLSPVPNLVHRYPNRVLWLVSARCAAHCRFCTRK
                     RRWRRPVPMTRDRIEEGLEYIRAHRA"
BASE COUNT         1082 a         1782 c         1931 g         1063 t
ORIGIN      
        1 acgccccgag ttgaggggat tgagaccagc tctcggcggt aatcacttaa tcccaaactg
       61 gtgctccgga aggacgcccc gatttgaggg gattgagact agtgctggcc aatgcagcgc
      121 tcagattcac ctgatgcctc cggaaggacg ccccgatttg gagcacgggg tcaggtctcc
      181 gtatggctta ttggctccga ccggagtgcc gagtgcggga gaaccgcttg ctcggttcgg
      241 agggaggggc ggtgcaagcc aatgcgccgt tcctacccct atcggatggg tccgaaagaa
      301 ggttctgatt ggatggggtc cacggggttt tccacctcct cgggaacggg ggggacgtct
      361 ccgggtcggg gtctcatccc tcacccccca accacctcct cggggacggg gggcatcggg
      421 cagcggggcg gggttcgggg cggactgcgg cccttcctca ggacacgggg atttcgtaga
      481 cgggaacggg ggtctcgagg cccttgaccc ggatttcccc cagggacctc gaggggacgt
      541 gatctcgcac cagctcgtag gttcgccggg agaccaggat gcctcccacg ggggcgttgg
      601 attcgagcct ctgggcgagg ttcacggcgg atcccagcac ggtgtaggag agcctctttt
      661 tggatcccat gtttcccacc accacctccc cggtgttgat gccgatgcgg atcttgaggg
      721 gcatgcctcc gtcccgggcc cacgcggcat cgagctcccg gaccttcttt tgcatgtccc
      781 gggccgcccg caccgcccgc agggcatggt cctcctgagg gtccgggtcg ccgaaaaaca
      841 ccatgagacc gtctccgata tacttgtcca ccgttccttc atgccggaac acgatctcga
      901 ccatggaatc gaaatattcg ttcagggact tctggatctg ctcgggcgtc agccgggccg
      961 agtgggtggt gaacccctgg atgtcgctga aaaggattga gagctccttt cgctgccccc
     1021 tcgaggccaa aacgtcgggt cttgccacca gctttttcac cacggagggg ggaaagtaag
     1081 cctcgaagag cctgcgcagc acggcctttt cccgggatac ccggatgtag cgcaccgcgg
     1141 tgatgctgcc cgccgagagc aggatggccg taaggggccg cagcagagga atgagggtgt
     1201 gggcgtagag gaacagggcc agggccagtc ccgaaacccc ggcggccagg gccaggcaca
     1261 ggaggggaaa gccgtaggag gatgccaagg cggccaggac cagcacgcac cccgccaggg
     1321 ccccgtccag gagaagtccc tggagaaagc tcaactcctg cagaaaggcc ccggaaagga
     1381 tcgtgttcaa gacattggcg tgaagaccgc tcaggggcag gttcgcgtcc gtgggcaccg
     1441 gtccctggtc cgccgacccc gtggccacgt cggatacaat gacgatcttt cccgaaagct
     1501 cttccttcca cagggccaat tcctcccggt cttcggacgc ccgatagacg gcggcgaagt
     1561 tgtagtgctt cattcgttcc cacgggcccg cgtagttgat gcgcaggttt ccccgggcgt
     1621 cgacgggaat gcggaggtcc cggggctttt cccacagggg atgccgggcg ttctcgagaa
     1681 gaacgtgttt ccccggccgc aggacgatcc gctcgggggg aacgcccagg tagtcgcagg
     1741 cgacccgaaa gggaaaactg gggtagaatc cgccctggaa ccgcaccacc agagggaccc
     1801 ggcgaaagac cccgtccggg tcggcggtca cgttgagaaa cccgactccc cgggaacgtt
     1861 cggccagttc ggaaaaggtg gtgagggggt ccgagccccg gtacaggccc gaggcatctc
     1921 cctcgacccg cagcttccag cgcccggcct cgagagactc gatccccgcc gtcttctgga
     1981 cggaacttcc ccccggtccc tccaccgcgt ccagcacgaa agccatgccg aaataggcgt
     2041 tggcggactt ccgggcggcg gaaagaaacg cctcgtcttc cgtttcgttg gttcgggccg
     2101 gaaagatgaa atcgtaggcc tgggcgaagg tgcccatggt tcccaggttt tccatgaccc
     2161 gggcgtaatg ggatcgattc aggtagaaat tcccgagccg ccggatggtg gaattggtga
     2221 cgtccacgtg gaccaccgtt ccgtcatagg gcggctggaa ggaaggaagg gaagatcgaa
     2281 gaacaaagaa ccggtcgaag acctgttcgt tccaggaagg gagcacccgc ggaaacagga
     2341 aaaacagcag gtgcgatccc agaaaggaga gtacgatcca gaagaccgtg atccgtcccc
     2401 gccgggaatc actggtgctc gatggtgaag gtcttggcat agaaacccgt ggtggaagag
     2461 atcacctcgg cgatgggatc gatgctctgc cggttttccc cctgggcctt ctcgatgcct
     2521 cggatagctc ccccgatggc cacgggacgt tcggcggatt cggcgaattc ccggtggagc
     2581 agtcgttccg ttcgaatctg ggccaggatc tgagtggtga ggtccggctt cttttcgggg
     2641 tccgccgatt cataggtctt gagaagggtc tcgagctcga cgagcctgcg ggcggaggcg
     2701 agcaggtaga aggtctcgcg ccccgggtgg gtatcgagtt cgaaccaccg ttcaccctgg
     2761 ggaatgtagt acctcttgtt cagctcgaac ccggccggga attgctcgag ggagtagggg
     2821 aaaagccgga tcatttcccc ctgggagttg tgatagatga cgtagacgaa gcaccgggtc
     2881 cggggttcca ccatcatttt gaagtggtct ccggacttca tggaccggtc ttcccggatg
     2941 ggttcgagcc gaagggtgag cccttcgtcg atgagggtcc cgaaggccca tcggaaggtg
     3001 acggtctggt cctgggagtg tgcgaaagag ggggaaagga ggggaaaaag ggacgccaga
     3061 atgaggaaca cgggaatcag ggtcccccaa agggtcccgc gggtttgaat ccgtcgcatg
     3121 gtttcctcct gctcatcatc cttccccggc ccctcccgtg gaaaaacggg agaaagctcc
     3181 tttcccgagg gggggagagg gcggggcgag gggttcttgc tttgggacag gccctagggt
     3241 tcccgcacca ggcggaagcc caggtcgtcg ttcatcccgt tgggaaggcc gcttccccgg
     3301 tcggcgcacc gcacgcttcg agggcttccg tgccagtagc cgccccgaat gacccggtcg
     3361 gctcccacgc ccgcattcac gtagacggga ccgtttcggt cgtgacgggt gtagacgtcg
     3421 atgccgtagg tatcctcgca ccattcccag acgttgccca gcatgtcgta gagcccgaag
     3481 gcattgggct ggaaggcggc caccggggaa gtggcggcgt atccgtcgtc acaatcgtgg
     3541 ctttcttccc atccccactg attccgggcc gtgtggtccg ccacgttctc gaaggtgcag
     3601 gcccggttgg ggtcgtctcc ccagtagcgg gccgcgtcgg aacctgctcg gcaggcgtat
     3661 tcccactcgg cttcggtggg cagccgaaac ttgtacttgc cgccgttctg ggccttgagc
     3721 cactgggcaa acccgttggc atcttcccag gtcacgtgga ccgcgggctg gttgtccccg
     3781 ttgagggaaa agccgtcgta gtcccggctg tcgtggttgg gctggaactt gcgaaactgc
     3841 ccgttggtgg tttccgtctt ggccatccag aagccttcga ggcagacctc gtggaccggc
     3901 ccctcgtcgg cgtccctgcc cgcttcggtt tcggggctgc ccatgttgaa acatccgccg
     3961 ggaatccaga ggaactccat gcccgaaacg ggttctttcc aagactttac ggccttcggg
     4021 gaggcctccc cccgggaagt cgaagaagcg accaccgtgg gcttgttttc ggcaaccgac
     4081 accggggccg ccgcgacgga cccgggcatg aagaagaaat ccccctcgag ggaggaagat
     4141 tcccagggga cctgtcttcc ctgggtgcct tcgcgcaccg ccgagcgcac ctgcttgaac
     4201 acgtcctcga tcttgactcc cggggctttc atgacgcgaa tgagttcccc ggtgtacacc
     4261 ccgtggtctt cgggtccgtc gttggccacg gagcccgggg ccgtggcgta ggcgatgatg
     4321 gtgccgatgg gggcgttgac ggatgccagc ccctgggccc cggaccggaa gctgcgggcg
     4381 aagggattgt cccggcatgc gtccaaaatg acgatgttga gccggtttcg ggcatactcc
     4441 atttccgaga gcaccgaacc gacgtccacc ccttcgtact cgacgtgctt ttcatgttcg
     4501 atcttggcgc cgatggggat cagataattt tttccgtcca cctgcatgcc gtgtccggca
     4561 tagtaaaaaa gccccacgcc ccctttttgg agcttctggc cgaaggcctg gatttcccgc
     4621 ttcatgtcct tctggctgag gttttcccgc tcggaaacct cgaagcccac ctcggccagg
     4681 gcccgggcga tggcccgggc atcgttgacg gggttgcgta ggggcgaggt ctcgtaagac
     4741 ccgtttccaa tgaccagggc cgtccgctgt tcccctccgg ggacggcggc cgggctcgag
     4801 ggcggccgac ccgtcttttc cattccccac gcggggttcg gcgcccagct caaagcacag
     4861 aaagccagca agccgatcat gccgacaact cggttcatag atctccttaa cgctcgaacc
     4921 cagaaagcaa aacagggagt cagcccaccg gaaggtcgga actccctttc ccaatcgttc
     4981 aataggaaat actcctcgaa ctgccgaagc aggccgaaaa cactagcaac acacggatat
     5041 tcccctaccg cgcaaatgca gggcgaattc tattcccgcc atttgccgaa atcaagtctt
     5101 tcgtgttatc agttcccggg cgggtcggga atcgagggcg ggaacccggg acgccaggga
     5161 atagggaaac cccgcgggcc caaaggacgc aaatcggcga cggcgtcgtt cacgggcctg
     5221 ggggcggaaa ccgcgtggga gcaagggacg caaagaacgg aaggaacgaa gggggcggga
     5281 tggacaccca ggacaccgat ccgggttggg cgattcggag agcatcagcc cgtcatggcc
     5341 gccaaggccg gcatccggcc ggtgggtatg gacgctcttc ccggatttcc gcttccgcgg
     5401 gaacgaggag gcgggatccg tgccggccga atcgtgcaac cggggaccga gtgaaaagcg
     5461 gggggccgaa ggcatgaaac ccggggacgt tttctccgtg aaggatcccg aaatgctgcg
     5521 ggggatggaa gccgtgtgcc ggcgctaccc ctttcgagcg ggccgctacg tgtgcggtct
     5581 cgttcgtcat ccgggagatc ccgtctggaa gcagatcgtt cccgacccgg cggaactcga
     5641 ggacgaggac ggcttcgagg acccccttgc cgaagaaagc ctttcccccg ttcccaacct
     5701 ggtgcatcgc tatcccaacc gggtgctgtg gctggtgtcc gcccggtgcg ccgctcactg
     5761 ccggttttgt actcgaaaga ggcgctggcg ccgtcccgtg cccatgaccc gggaccggat
     5821 cgaggaaggc ctcgagtaca tccgcgccca ccgggcgg
//
LOCUS       DAOOVC010000204         5818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7845_length_5818_cov_3.746486, whole genome shotgun sequence.
ACCESSION   DAOOVC010000204 DAOOVC010000000
VERSION     DAOOVC010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7845_length_5818_cov_3.746486"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1817
                     /locus_tag="PLM79_16725"
     CDS             <1..1817
                     /locus_tag="PLM79_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88002.1"
                     /translation="PVCLVRRSKKEIVDGKEKLPSKEKPCEICKNRRTHRLEKWLGRD
                     FGSDSIWISEVADANDRVALITMSLDIEPWLDGTRLDSLRAQAIAEWRKFNPVLKNQP
                     NPIDASTCFESLFQYIKGKLQSFDKADPVLSSLQEGFQYETDWLPFFSKIVEDRVPND
                     HLTWDNLDESGRAAWLSHQLFRKLASPGRIYRFERQTEDFFRELLAEFRDIAAADSNR
                     WRVRRLVIKPDSIGSSGSCKDRQVYNGRYGDAPVSLLYRKKDNDFLTICNLARLLKPE
                     DGKDSLNGITLELKADDSMEVKQLKVKSAEDVPDTLGIYHPVIPLELSPVRFRVLLPL
                     EAASDCVDKAIEKWKEQFARVWDRLPLRVGVVAFPRMMPFQAVIEAARNIEGDLDRIK
                     KTETWRVAECKTRDGVTALCIKSLDHRHELLQTIPIRLPDGREDVFYPYLAVEDKQVR
                     FPLDFKHPDGQVFRHAKDVGSGDGVLVHPSLISTIFMDGTARRFEPLSPRQLTEWLQM
                     RDLWRLISRHAPSQTALRGAWSEIVERREDWQGPDGTWLEEGKTAWLDFARAVFHERL
                     SLRGACLETLVKAAGDELLDWSLEWHMGVLKKQVSGGD"
     gene            1817..2878
                     /gene="cmr4"
                     /locus_tag="PLM79_16730"
     CDS             1817..2878
                     /gene="cmr4"
                     /locus_tag="PLM79_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOV88003.1"
                     /translation="MTNGGNTIKKTKSFETYQFAGMALDPIHVGSGGTRLGRVDNTIV
                     RDPVTRIPKIPGSSLAGVIRAYVAMAKEKYPNCVGLGQPERDGSGGHCGKADCPVCTV
                     FGFAKGIDASGGFAGLAAFSDMQVLLFPVASQLGPRWITCPIALRQTGIEAFSELADL
                     PDEQAVYRKLVKSTETEQKNGTKQDKSQSEASLNLGWLLLPVKTDWQQLDNISKELTN
                     LRIPEYIISRLGVVSDKLFVHIVNSNLEVRTSVAIDPATGAAEKRALFTYEALPRGSV
                     LFWELMCRNPKHFKIGQDDVKAVDSPEKVKDVVTAAHAYLEHLGIGGMGSRGMGRLRV
                     LNNSVSKTEHASLSEKETP"
     gene            2878..3273
                     /locus_tag="PLM79_16735"
     CDS             2878..3273
                     /locus_tag="PLM79_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88004.1"
                     /translation="MNALTFENLDLACAMAGKKIAAKPSKELENLVTNALAVSEDQGL
                     YALFLFLKAQTEKNKEMAKGICDQLFTFLKETPQQAPLLSDNADVFISLQQTAKDLDK
                     LLLARDLTRQALIYARYHARVIEESEVRK"
     gene            3270..4238
                     /locus_tag="PLM79_16740"
     CDS             3270..4238
                     /locus_tag="PLM79_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88005.1"
                     /translation="MTWTVFRWVWRLEAPLFVGMPPAGALNRCRLYLPARAMHGAITS
                     ELARMDGEKKSKFPDYGKFGKEVGENCRFTYLYPAEKLDGKFLAWVPKYLRADHDKKT
                     RDGEQKIGLRWHRMDGKRDFSDRDFRRSLLDSRSGTAIAPESDSASEGTLRETECINP
                     WWRDVSGARTDPKPVLLLGYVFLKNNGFRRQLDNIDTLFVGGDTRYGLGKIHRVELTD
                     LSADISVFGKKVYLDGEEPEMQSNIVWGHALEGSQNQTMKMQGVKELPGRWEIVNLWK
                     RRRENGKEMEEFPAWAPGSCFENTGSQSLEYCWAIDTYGIWEYVPH"
     gene            4275..4910
                     /gene="cas4"
                     /locus_tag="PLM79_16745"
     CDS             4275..4910
                     /gene="cas4"
                     /locus_tag="PLM79_16745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOV88006.1"
                     /translation="MYDEEDLLQLSALQHFLFCPRQCALIHIEQIWEENLYTIEGELL
                     HQRAHSGKSEGRPMKRTEFGMPIRSLELGLSGKADAVEYRADGGIVPVEYKRGRPKKG
                     GADEVQLCAQALCLEEMLGVSIPEGALFYGKVRRRKVVPFSCELRTYVRETTARLHEF
                     IRAGRTPPPEYDEKKCPRCSLLSVCLPKKLSRPASVERYIERMLNEKGDTP"
     gene            4907..>5818
                     /gene="cas1c"
                     /locus_tag="PLM79_16750"
     CDS             4907..>5818
                     /gene="cas1c"
                     /locus_tag="PLM79_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOV88007.1"
                     /translation="MKRLLNTLFVMTQNAYLKKEGETVVVRVGRETKLRVPIITIGSI
                     VCFGDVLMSPSLMGFCAKSGVTISFLSVYGRFLARVQGPVSGNVLLRKEQYRISDDKV
                     KSAQVARTITAAKIANCRTVLLRALRDHGDTIRADETSKTVNVLKRNLELVLGEHDLE
                     RIRGIEGDSARVYFSVFNNLIVSQKADFSFRDRNRRPPLDRVNALLSFVYTMLYHDMR
                     SALEATGLDPAVGFLHRDRPGRMSLALDLMEEMRPFIADRLVLSLINMKQVDRKGFTV
                     SESGAVLMDDACRKTVVASYQKRKQDAV"
BASE COUNT         1442 a         1399 c         1676 g         1301 t
ORIGIN      
        1 gtcccgtatg tcttgtgcgc agaagcaaga aagaaatcgt tgatggaaaa gagaagcttc
       61 cgagcaagga aaaaccgtgc gagatttgta agaatcgacg gactcatcgc ctggaaaagt
      121 ggttggggag agacttcgga tcagactcca tctggatcag tgaggtggcc gatgcgaatg
      181 accgcgtagc ccttatcaca atgagtctgg acatcgagcc ctggcttgat ggaacccggc
      241 tggattctct cagagctcaa gctattgcgg agtggcgcaa gttcaatcct gtcttaaaaa
      301 accagccaaa ccccatcgat gcctccactt gtttcgaaag cctctttcag tacatcaaag
      361 gaaaactcca atcctttgac aaggcagacc ccgtgctgag cagcctacag gaggggttcc
      421 aatacgagac agattggcta cctttcttct ccaaaatcgt cgaagatcgc gtccccaacg
      481 accatcttac atgggacaat cttgatgaaa gtggccgtgc cgcatggctt tcacaccagt
      541 tatttcgcaa gctcgcctcg ccaggccgta tctatcgctt cgagcggcag accgaagatt
      601 tctttagaga acttcttgcc gaattccgcg atatagcggc cgcagactcg aatcgctggc
      661 gcgtgcgccg tctggttata aagccggata gcatcggttc ttccggttcg tgtaaagacc
      721 ggcaggtcta caacggacgc tatggagatg cgccggtaag cctgttgtat cggaaaaagg
      781 acaacgactt ccttaccatt tgcaaccttg cccgtctttt gaaaccggaa gacggcaagg
      841 atagcctgaa cggaatcact ctggagttga aagcggatga tagcatggaa gtcaagcagc
      901 taaaggttaa atcggcagag gacgtgccag atactctggg tatctatcat ccggtgatcc
      961 ctcttgagct ttcgccagta cgctttcgag tgcttttgcc gcttgaggcc gcatcggatt
     1021 gcgtagataa agcgattgaa aaatggaagg agcaattcgc ccgtgtctgg gaccgtctgc
     1081 cgcttcgagt tggcgttgtg gccttcccca ggatgatgcc gtttcaggct gtaatagaag
     1141 cagcgcggaa catcgaaggt gatctggata ggattaagaa gactgaaacg tggcgggtag
     1201 cggaatgcaa aacgcgggac ggcgtcaccg ccctttgcat caagtcgcta gatcatcgac
     1261 acgagcttct tcaaaccatt cccatcaggc tccctgatgg gagagaagat gtgttttatc
     1321 cataccttgc cgttgaggac aaacaggttc gttttccgtt ggactttaag cacccggatg
     1381 gccaggtttt caggcatgcg aaggacgtgg gaagcgggga cggggttctt gtacatcctt
     1441 cacttatttc cacgattttc atggacggca cggccagacg tttcgaaccc ttatctccca
     1501 gacagctaac ggaatggctt cagatgaggg atctctggag gctgataagc cggcatgcac
     1561 ccagtcagac cgcgctccgc ggcgcatggt cggaaattgt cgagcgacgc gaggactggc
     1621 aagggccgga cggaacatgg ctcgaagaag gaaagacggc atggctcgat ttcgcacgtg
     1681 cggtgttcca tgaacgcctg agtcttcgag gtgcttgcct tgaaacgttg gtgaaagccg
     1741 ccggagacga actcctggac tggagcctgg aatggcacat gggcgtattg aaaaagcaag
     1801 tgtcaggagg tgactaatga caaatggtgg aaatacaatt aaaaagacaa agagttttga
     1861 gacctatcag tttgccggca tggcgctcga tcccatccat gtcggctcgg gcggaactcg
     1921 gctgggccgg gtggataaca cgattgttcg cgacccggtg acacggatac caaagattcc
     1981 cggctcgagc ttggcgggag tcatacgagc ctacgtagct atggcaaagg aaaaataccc
     2041 caactgtgtc ggccttggtc agccagagcg cgacggttct ggcggtcatt gcggaaaggc
     2101 cgattgtcct gtgtgcacgg tgttcggttt tgccaaaggc atagacgcaa gcggtggatt
     2161 cgcaggattg gctgcgttca gcgatatgca ggtgctgctt tttcccgtgg cctcacaatt
     2221 gggaccacga tggatcacct gccccatagc attacggcaa accggcattg aggcattctc
     2281 ggaactggcg gacctgcctg atgagcaggc tgtttaccgg aaactcgtca aaagcaccga
     2341 aaccgaacag aaaaatggca ccaaacaaga caagtcgcaa agcgaggcat cattgaacct
     2401 cggctggctt cttcttccag tcaagactga ttggcaacaa ctggataata tatccaagga
     2461 gttgacaaac ttgcgtatac cggaatacat catcagccgc cttggtgtcg tttccgacaa
     2521 actgttcgtc catattgtga acagcaacct tgaagtacgg acctcggttg ccatcgatcc
     2581 agccaccggc gcggccgaga aaagagcgct gtttacctat gaagcccttc ctcgaggctc
     2641 agtgcttttt tgggagctaa tgtgccgaaa tccaaaacac tttaagattg gtcaagatga
     2701 tgtgaaggcg gtcgactcgc cggagaaggt caaagatgtc gttactgccg ctcatgctta
     2761 tcttgaacat ctcggtatcg gcggcatggg aagccgtggc atgggacggc tgcgcgtgct
     2821 gaacaatagt gtgagcaaaa cagaacatgc gagcttatcg gaaaaggaga ccccgtgatg
     2881 aacgcgctaa cctttgaaaa cctggacctt gcctgtgcca tggctggcaa gaagatcgcc
     2941 gctaagccgt caaaggagct tgagaacctc gttacgaatg cacttgctgt ttcggaagac
     3001 cagggtctgt atgccctgtt tctatttctc aaggcgcaaa cggagaaaaa caaggaaatg
     3061 gcaaagggta tttgcgacca gctctttaca tttctaaaag agacgccgca gcaggctcct
     3121 ttactttccg acaatgccga cgtatttatc tcgcttcagc agacggccaa ggaccttgac
     3181 aagttactgc tggcacgcga tctgacccgg caagcactca tctatgctcg ttatcatgcg
     3241 cgagtcatag aagaaagcga ggtgcgaaaa tgacctggac ggtgtttcga tgggtctggc
     3301 gactggaggc gccgcttttt gttggcatgc ctccggcagg ggcgttgaac cgctgccggc
     3361 tttatctacc cgcacgggcg atgcatggag ccattacatc cgaattagct cggatggatg
     3421 gagagaaaaa gtccaaattc ccggattatg gaaagttcgg caaagaagtg ggagaaaact
     3481 gtcgtttcac ttatctctat ccagctgaga aactggacgg taagttcctt gcctgggtac
     3541 cgaaatactt aagggcggat catgataaga agaccagaga tggtgaacaa aaaatcggat
     3601 tacgatggca tcgtatggac ggcaaaagag acttttcaga ccgagatttc aggcgtagcc
     3661 tactagactc ccggtctggt accgcaatcg ctcctgaatc ggactcagcg tctgaaggta
     3721 cactccgaga gacagaatgc atcaatccct ggtggcgtga tgtatctggt gctcgtacag
     3781 atccgaaacc tgttcttctc ctggggtacg tcttcctgaa gaataatggc tttcgaaggc
     3841 agttggacaa tattgatacc ctctttgttg gaggcgacac aagatatggc cttgggaaaa
     3901 tacatcgcgt cgaattgacc gatctatcag ctgacatttc ggtctttggg aaaaaagttt
     3961 atctggatgg agaagagccc gaaatgcaga gcaacatcgt ttggggacat gccctggaag
     4021 gtagccagaa tcaaacaatg aaaatgcaag gagttaagga acttcctggc aggtgggaaa
     4081 ttgttaatct ctggaaaaga agaagagaaa atggaaaaga gatggaagaa ttcccggcct
     4141 gggcgcccgg atcatgtttt gaaaatacgg gaagccagtc attggaatat tgctgggcaa
     4201 tagatactta cggaatttgg gaatatgtgc cccattgata cgtaagcgtg tgaggtgacg
     4261 cgagggaggc ggccatgtac gatgaggagg atcttctgca gctttcggcg cttcagcatt
     4321 ttctcttctg cccgaggcag tgcgccctta ttcacatcga gcagatctgg gaggagaatc
     4381 tctacacgat cgaaggagag ctccttcacc agcgggccca ctcggggaag tctgagggac
     4441 ggccgatgaa acggactgag ttcggaatgc cgatccggtc ccttgagctt gggctttctg
     4501 ggaaagccga cgcggtggag tacagggcgg acggggggat cgttccggtc gagtacaagc
     4561 gggggagacc gaagaaggga ggagcggatg aggtgcagct ctgcgcgcag gcgctatgcc
     4621 ttgaggagat gctgggcgtt tcgattcccg agggcgcact gttctacgga aaggtgaggc
     4681 ggaggaaggt cgtccccttt tcctgcgagc tacggacgta cgtgcgggag accaccgcga
     4741 ggcttcacga gtttattcgt gctggcagaa cgccgccacc tgaatatgat gaaaagaaat
     4801 gtccgaggtg ttccctcctc tccgtctgcc ttccgaagaa gctttcccgg cctgcctccg
     4861 tggaacggta tatcgagcgg atgctcaacg aaaaggggga tactccatga agcggcttct
     4921 taacacgctt tttgtcatga ctcagaacgc ttatctgaag aaggagggtg agactgtcgt
     4981 ggtgcgggtt gggagggaga cgaagctccg ggtaccgatc atcacgatcg gttcgattgt
     5041 ctgtttcggg gatgtgctca tgagtccgtc tctcatgggt ttctgcgcga agagcggggt
     5101 gacgataagc tttttaagtg tgtacgggag attcctcgcc cgggtgcagg gacctgtctc
     5161 tgggaacgtg ctgctccgca aggagcagta ccggatctcc gacgacaagg ttaaatccgc
     5221 acaggtagcg aggacaatca ctgcggcgaa gattgcgaat tgcagaactg ttctcctccg
     5281 cgccttgcga gatcacggtg atacaatacg agccgatgag acctccaaga ctgtaaacgt
     5341 attgaagaga aatctggagc ttgttctcgg cgagcatgat ctcgagagga tacgggggat
     5401 cgagggagat tcggcgcggg tttattttag cgtgttcaat aatctcattg tctcccaaaa
     5461 ggctgatttt tccttcaggg atagaaacag gcgacctccc ctggaccggg tcaacgctct
     5521 cctttctttc gtctatacaa tgttgtacca tgatatgaga tcagccctgg aagcgacggg
     5581 gcttgacccg gcagtcggat ttctccacag ggaccggccg ggaaggatga gcctcgccct
     5641 cgatctcatg gaggagatgc ggccgtttat cgcagaccgt ctcgtcctct cacttatcaa
     5701 tatgaaacag gttgatcgga agggttttac cgtctcggaa tccggggccg ttctgatgga
     5761 tgatgcatgc aggaaaaccg tggtcgcctc ctatcagaaa cgaaaacagg atgccgtg
//
LOCUS       DAOOVC010000205         5759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7938_length_5759_cov_6.136571, whole genome shotgun sequence.
ACCESSION   DAOOVC010000205 DAOOVC010000000
VERSION     DAOOVC010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7938_length_5759_cov_6.136571"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PLM79_16755"
     CDS             <1..1825
                     /locus_tag="PLM79_16755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HOV88008.1"
                     /translation="GNVTRRLYSDSTDLQFGYDARNLPTSSNAIKLERDAEGRVIASE
                     SEGAVFGTAYDDGGRIKSTSYANGAFSVTYTYDPGTGLLANVADDLTHTSVSFFYDGD
                     RRLIGIERSNGVNGSYEWDEAGRLTHIREGSLIDGHITRDASGQVTNMMLDAPLNPDR
                     FLVQDVQILSFDAASRISSKGYRYDPRGRLILSPGHTFTWDGASRLVELDDVQLGYNG
                     FGDLVLRTSGTETTRYFHNYAVDTRPILAERNEADLRFLRYYVWTPEGSLLYMIDGGQ
                     GNSVYFHHFDPSGSTLALTNAEGALTDAYAYNPYGILLRHEGNNPQPFTFVGQYGVRQ
                     EGAGGAIYQMRERYYDASTCRFLSRESDWPQTVDPRMLNPYQYAFNNPISFVDVTGRG
                     PVREFFKKVWNLIWPGAKSRAKEGGKKLVTEGAEEAVATAAEFALGPAVGGVVELLQS
                     TGGGAVSSTAGLLFFTQPRLGDGTIHESIISEKDLPILSDTGLYQRMSEAQVKIDDLY
                     ANLRDPGKLWDKHDLSLARHRMSDLTESLAIMKQEANRRRIIGLRELPWKLLRERYRA
                     HTGQPEEMKQISTAAISKSIQEGDFERARLWLGHLREWESR"
     gene            2283..3269
                     /locus_tag="PLM79_16760"
     CDS             2283..3269
                     /locus_tag="PLM79_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOV88009.1"
                     /translation="MERVQFGQTDMEVSVIVLGTWVTGGWAWGGSDETESLNALLKAF
                     EMGINFIDTAPVYGFGKSEWIVGNALKEWGSRERVFVATKCGLEWDEQERIRRNSKPE
                     RIYREADESLRRLGIEQIDLYQVHWPDTGVPFEASMEAMLRLRELGKIRYIGLSNFNR
                     EQVEECLRHAAVVSLQPPFNVFERGAEEELLPFCADQGLATLVYGGLCRGLLTGKFKG
                     DEKFPRGDLRRADPKFKPDRFMQYVKAVRELEKLAAGYGRTVAQFSLRWALQQKGVTT
                     VIAGARTAAQVEDNVGVSGWKIEPEDLARVEEILNRHIKTPVGPEFMSPRAD"
     gene            complement(3266..4507)
                     /locus_tag="PLM79_16765"
     CDS             complement(3266..4507)
                     /locus_tag="PLM79_16765"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88010.1"
                     /translation="MIEGVPSGMSENKAYQRHLKRVVVGAVHRWSVVVPPEFESLCRR
                     ELETLGLAAAGSGEGIVEFSGKLRDCYAANLHLRTASRVFCSLDPFRAGIAEELRHKA
                     SAVPWELWLNPRIPVRLEVHVRHSRIEHEGLVGEMVLSGLRKRFRERGFPVPDEWRPP
                     PVPAEVSPEDARDSWRQRIYVHLRNNHCVISLDTSGHHLHKRGYRLRHGGAPLRETIA
                     AAVLYRVGWNGSLPLVDGMCGAGTLAIEAALMARNLPPGLTRPFLFQHWPSYGKATWE
                     YLCRKAREGSLERKPGPLVALDRDPDVIETARENASRAGVADDVLWECRDFFSFRPGE
                     RGLRPGLLLLNPPYGRRLRDGGESLYRELGAHLREHFRGWQAAVLAPSLPVAGRMKIH
                     PMRFWKIEHGGLPVVVGMARI"
     gene            4816..>5759
                     /locus_tag="PLM79_16770"
     CDS             4816..>5759
                     /locus_tag="PLM79_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOV88011.1"
                     /translation="MEDRLSIGLLHLDIEHGQTVKNRRSLLTWVEKAARSGARVVVAP
                     ELALSGYSFESLDEAASLAESLAEETIWLLCEAARRWKILLCFGFAERDPSTNILYNS
                     ACVVGPDGRVAAHHRKNVTDRRWACPGTPTQQNLLDTPWGRVGLLICADTYYGLLPRS
                     LAIQGADLLLVPANWPPSGMDPRKIWRARALENGMGVIACNRTGVDRHMDCRSSSSYA
                     VTGEGDVLLDGISESSCLWMVDYPLENGRLASRHRQAAMTRRRPEDYQALYLDVNGMQ
                     DFAGFWRLPRGGELDIRCVVPEGPEQTQAALEAAVRQAL"
BASE COUNT         1181 a         1763 c         1767 g         1048 t
ORIGIN      
        1 gggcaacgtc acccgcaggc tctactccga cagtaccgac ctgcaattcg gctacgacgc
       61 ccggaatctg ccgacttcct cgaacgccat aaagctcgag agggatgcgg aaggccgggt
      121 gattgcttcg gaaagcgaag gagcggtctt tgggaccgcc tatgacgacg gaggcaggat
      181 caaaagcact tcctacgcca acggcgcttt ttcagtcact tacacttacg atcccggaac
      241 cggccttctc gccaatgtcg ccgacgatct cacccacacg tccgtctcgt ttttttacga
      301 cggggaccgg cgactgatcg gcatcgagcg ctccaacggc gtcaacggct cctatgagtg
      361 ggatgaggcg gggcggctca cgcatattcg agagggtagc ctcatcgacg gccacatcac
      421 cagggatgcg tccggccagg tgacaaacat gatgctcgac gctcccctga accccgaccg
      481 cttccttgtg caggatgtgc aaatcctctc ctttgacgcg gcgtcccgaa tttcttccaa
      541 gggatacagg tacgatccga gggggagact catcctatca cccggccaca ctttcacctg
      601 ggacggcgct tcgcggctgg tagagctcga tgatgtgcag ctgggctaca acggcttcgg
      661 agacctcgta ctccgcacca gcggaaccga gacgacgcga tatttccaca attacgccgt
      721 cgacaccaga ccgatcctcg cggagcggaa cgaggcggac ctccgattcc tacgctacta
      781 cgtgtggaca cccgagggct cgctcctcta catgatcgac ggcggacagg gaaacagcgt
      841 gtatttccac cacttcgatc catccggatc caccctggct ttgacaaacg cggaaggcgc
      901 gctcacggac gcttacgcct acaacccgta cggcattctc ttgcgccacg aggggaacaa
      961 tccgcaaccg ttcacctttg tcggccagta cggtgtgcga caggaagggg ccggcggggc
     1021 catctaccag atgcgggaac gctattacga tgcatcgacc tgccggtttc tctcccggga
     1081 atcggactgg ccccagacag tcgatccgcg gatgctcaac ccttatcagt acgctttcaa
     1141 caatccgatc agctttgtgg acgtcaccgg gagggggccc gtcagagagt ttttcaagaa
     1201 ggtctggaat ctgatctggc ccggcgcgaa atcgcgcgcc aaggaaggcg gaaagaaact
     1261 agtcacggag ggcgctgaag aagcggtcgc gacggcggcg gagtttgcgc tgggtccagc
     1321 ggtcggggga gttgttgaac tactgcagtc aacaggcggg ggagctgttt ccagcactgc
     1381 ggggctcctc ttctttacgc agccgaggct cggcgatgga acgatccatg agtcgatcat
     1441 cagcgaaaag gatttgccca tcttgagcga taccgggctg tatcagcgca tgagcgaagc
     1501 gcaggtgaag atcgatgatc tatacgccaa tttgcgcgac cccgggaaat tgtgggacaa
     1561 acacgatctg tcgttggccc gtcaccgaat gagcgatctt acggaaagcc tggcaattat
     1621 gaaacaggaa gccaaccggc gcaggatcat cgggctcagg gagctcccct ggaaactgtt
     1681 aagagaaaga tacagggccc acaccggcca acccgaggaa atgaagcaga tcagcaccgc
     1741 cgccatcagc aagagcatcc aggagggcga cttcgaaagg gcgaggctat ggctgggcca
     1801 cttgcgggaa tgggagtccc gttaggaggc actttcgcat tcgcctgtgc gggaaggtct
     1861 gcgtacggac attgcaggtc ggcctttatg tggccccggg aagagctccc atgttggacg
     1921 gcgacacagc acaaggaatc acccaccatg gagtcgaatc cgagggaaag acgcctcgac
     1981 atgagcaggt cccggcgccc acgctcctcg cgggcgacgg ggcgcacctc caggccgtgg
     2041 agtgttgcca aacttgggtt ggtggacttg ttctcatgac tcgtgccact ctatgcggct
     2101 gatatggagg cggaagtccg acaagcacaa cttttccgga aatttccccg ttgtatccgg
     2161 ctgcgtgacg cagtcgtcct agggaggttc ggatgtgggc accaaagggg caagaggctg
     2221 tggtagagtg tgaagactcg gcgtagaggc ctaaagacac caccaaacaa aggggcggaa
     2281 ccatggaaag agttcaattc ggacaaacgg acatggaggt ttcggtcatc gtattgggta
     2341 cctgggttac gggcggttgg gcctggggag gatccgacga aacggaatcc ttgaacgccc
     2401 ttctcaaggc cttcgagatg ggaatcaact tcatcgatac ggcaccggtc tacggcttcg
     2461 gcaagtcgga atggattgtg ggcaacgccc tcaaggaatg gggctcccgg gagagggttt
     2521 ttgtggccac caagtgcggg ttggagtggg acgagcagga gcggattcga cgcaattcga
     2581 agcccgagag gatctatcga gaagcggacg agagcctccg acgcctgggc atcgaacaga
     2641 tcgacctcta ccaggtgcac tggccggata ccggagtccc cttcgaggcc tccatggaag
     2701 ccatgctgcg gctccgggag ctgggaaaga tccgctacat cgggttgagc aacttcaacc
     2761 gggaacaggt cgaggaatgt cttcgtcacg ccgccgtcgt cagccttcaa ccccctttca
     2821 acgtcttcga acgaggggct gaggaggagt tgctcccctt ttgcgccgat caaggcctgg
     2881 cgaccctggt ctacggcggg ctttgccgag gtctcctcac ggggaagttc aaaggggacg
     2941 agaaatttcc ccggggcgat ttgcggcggg cggatcccaa gttcaaaccc gaccgcttca
     3001 tgcagtacgt gaaagcggtg cgcgaactgg agaagcttgc agccggctac ggccgtacgg
     3061 tggcccaatt ctccctgcgc tgggcccttc agcagaaggg ggtcaccacg gtcattgccg
     3121 gagcgagaac tgcggcacag gtcgaggaca acgtcggggt gtccggctgg aagatcgagc
     3181 ccgaggacct cgctcgagtg gaagaaatcc tgaacaggca catcaagacc ccggtaggcc
     3241 cggaattcat gagtccccgg gccgactaga tcctcgccat gcccaccacg acgggcagtc
     3301 ccccgtgctc gatcttccag aagcgcatgg gatggatctt cattctcccg gcaacgggga
     3361 gggagggagc caggacggct gcctgccaac cccgaaaatg ttcgcgcagg tgggctccca
     3421 gctcccggta aagagactcc ccgccatccc ggagtcgcct cccgtaggga ggattcaaga
     3481 ggagcaaccc cggccgaagc ccgcgttccc cgggtcgaaa cgagaaaaaa tccctgcatt
     3541 cccagagaac atcgtcggca actcccgccc tggacgcatt ctcccgagcc gtctcgatga
     3601 cgtcggggtc tcgatccagg gctaccaagg gtcccggctt tctctcgagg gacccctccc
     3661 gggccttccg gcacagatac tcccacgtgg cctttccata cgacggccag tgctggaaca
     3721 aaaagggacg cgtcagaccc gggggaagat ttcgcgccat gagggctgct tcgatggcca
     3781 gggtgcccgc tccgcacatc ccatccacca ggggaaggct cccgttccac ccgacccggt
     3841 acaaaacggc ggcggcaatg gtttccctca ggggggctcc cccgtgtcgc aggcgatatc
     3901 cccgcttgtg gagatgatgc cccgaggtgt ccaggctgat cacacagtga ttgttcctca
     3961 agtggacata gatccgctgc ctccatgaat ctcttgcatc ttccggggat acctcggccg
     4021 ggaccggggg aggtctccat tcgtcgggca cgggaaaccc ccgctcccga aaccgctttc
     4081 gcaggccgga aaggaccatc tcccccacca atccctcgtg ctcgatgcgg gagtgccgga
     4141 cgtggacctc gaggcgaacc gggatgcggg gattcaacca cagctcccag ggtacggccg
     4201 aggctttgtg ccgaagctcc tcggcaatcc ctgctcggaa cggatcgagg gaacagaaca
     4261 cccgggaagc cgtccgaagg tggagattgg ccgcatagca gtcccggagc tttcccgaaa
     4321 actcgacgat cccttccccc gagcccgccg ctgcaagccc gagtgtttcc agctctcttc
     4381 ggcacaagga ctcgaactcg ggagggacca ccacgctcca gcgatggacg gctcccacca
     4441 caactctttt gagatgcctc tggtacgcct tgttttccga cattcccgaa ggaactccct
     4501 cgatcatcgg tctttcctct gccgttccgt tcaatggcga atcgatcacg atccgggcac
     4561 tgtttcatga actctcggat tccgcaaacg gtttcgatgc agggtcccca cctttccgat
     4621 tgtaaccgag gcctccggtt ttctcctctc actcgcggaa aagggcgcga attccactcc
     4681 cgtctcggcc cgaatattga taaggtggac ctcggacggc aaatcgcttg cggcaaaaca
     4741 cggaacgtgc cgggtcctgg aatcggcaaa ggcgggaccg ccgttcatcc aaggggtctt
     4801 tccacggagg gggctgtgga ggatcgtcta tccatcggac tgctccatct cgacatagaa
     4861 cacggacaaa cggtaaagaa tcgaaggtcc ctgctgacct gggttgagaa ggcggcccgg
     4921 tccggcgccc gcgtcgtggt ggccccggag ttggcccttt cgggctacag cttcgaatcc
     4981 ttggacgaag cagcctccct ggcggaatcc ctggcggagg agacgatttg gttgctgtgc
     5041 gaggcggcgc gccgctggaa aatcctcctc tgctttggat ttgccgagcg ggacccctcg
     5101 acgaacatct tatacaacag cgcctgcgtg gtcggaccgg acggccgcgt ggcggcccat
     5161 catcggaaaa acgtcacgga ccgccggtgg gcctgtcccg gcactcctac ccaacagaac
     5221 ctgttggaca ccccatgggg gcgggtgggg ctcctcatct gcgcggacac ctactacggc
     5281 ctcttgcccc gttccctggc catccagggg gcggacctgc tgctggtccc ggccaattgg
     5341 cctccgtccg gcatggaccc tcgaaagatc tggcgcgcca gggccttgga gaacgggatg
     5401 ggagtgatcg cgtgcaaccg caccggagtg gatcgtcaca tggactgccg gtcctcttct
     5461 tcctacgcgg tcaccggtga gggagacgtg cttctggacg ggatcagcga atcctcctgt
     5521 ctttggatgg tagactaccc tctcgagaac gggcggcttg cttcgaggca ccgccaggcc
     5581 gccatgaccc ggcgtcgccc cgaggactac caggctctct acctggacgt caacggcatg
     5641 caggacttcg ccggattctg gcggttgccc cggggcggcg aattggacat ccggtgcgtg
     5701 gtgcccgaag gaccggaaca gacccaggcc gctcttgaag ccgccgttcg tcaggccct
//
LOCUS       DAOOVC010000206         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7941_length_5757_cov_6.784286, whole genome shotgun sequence.
ACCESSION   DAOOVC010000206 DAOOVC010000000
VERSION     DAOOVC010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7941_length_5757_cov_6.784286"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="PLM79_16775"
     CDS             complement(<1..1785)
                     /locus_tag="PLM79_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOV88012.1"
                     /translation="MSFLARLLQAWTAWVLPRPRFVLGAALVAALASLILASQRLEIQ
                     TDQLELISPDHPLIALADRLEPFNFGEKTVLVLVLEAPKPEDAVAFVQELVPKIQRDR
                     THFEDVLYRVDPETMKQWALLYSEPLDLIHLRDKLDDSAGLIEGITRQPDLLIFLRLV
                     NQEMASRMVGELFTGFLDGPELADDRHEEKGPLDLRFLIQTLSGLSGYLSGTPHFTSP
                     WDSFFTPSSWNLDLEGYFWEAGKKYLLLFVVPEKEKEGFSRAQRSLDRLRSLIRETRE
                     NFPEVRAGVTGQAALNNDQMTTVLGDMTRATWLSLLGVLTILLLTFRSLRRSAFEVFS
                     LGVGICWTLGFATLVIGHLNILSVVFAPLLCGLGVDYGIHWFARYEEEERHVGLDRKA
                     VIRRVADRSGPGILLAGLSAACSFLPLVLTGFRGLMELGIITGAGILLILLADFTLLP
                     ALTVLFVEKRWDFAPSEFLSRNGDLLTFGRPTAVLILVCAGVLCLLGLAGASRVRFDL
                     NPLKLQAPRAEAVVWEKKLIRDSRRSPLYGAVFAESLEEVRTKSSSLEALPVVSEVES
                     ILTLLPQNQGTKIPLLRSMLAKVPNMKQT"
     gene            complement(1841..3253)
                     /locus_tag="PLM79_16780"
     CDS             complement(1841..3253)
                     /locus_tag="PLM79_16780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV88013.1"
                     /translation="MRRTRRGVKGRWMILFLFCLGVFAAEESPASEKPVLTLDQVIGM
                     AVEYSPQIREAEQDVKAAETDLAQAKAGQWAQMDITAFGGPVQDARTPVVLVTPSKDA
                     EGFLRGQIKNRDETSIGFFGRLELSIVQPLYTFGKISNRRDAAAHGLEAQKAAMEKKR
                     GEVILTAKEYYFSYIVARQGIEAADDADTFIQDARRRIKRLIELKAKNADESDLYKLE
                     AFEAEIQQFKVKAQTGAQMAYLALKKHIGYPEDKDFELDRKTLPKDTRALGSQEEYTR
                     KALSQRPEFEQIRRGIEARRALVEAAKADLYPSVFAAAVGSFAGAPGRERMADSYIED
                     EFNHAYMGAILGANWHFDLGIGQARVRKARAEHQKMLHTQEYAEQNIPLEVAKYYQDV
                     IESQASFQAYEKAAVAARKWIVSAFSNFDMGVGPAKDIFDAIDRYGKNQGEYLLSLYN
                     YHVSLAKLSYAVGEYRSQRY"
     gene            3480..4394
                     /locus_tag="PLM79_16785"
     CDS             3480..4394
                     /locus_tag="PLM79_16785"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOV88014.1"
                     /translation="MKNPPCSGPNSRKRRLIVNADDFGFTRGVNRAVERCFTRGTVRS
                     ASLMAGGSAFEDAARLARSLPGLGVGAHLVLTGLRAVSEPGSLPGLADSRGFLPPSPG
                     ALIERLLRFPDARASVFRELNAQLSRIVDAGIRPTHLDSHKHVHILPPVLRSVTDLAR
                     KYSVRWIRNPFEVENAGSLLRGVAPSHRKAFWKQHVKSLVARVFRPGFMGHVGTTGLR
                     LPDRFHGVSLTGLWNEEAALHTIGRLSPGLNEWMFHPGEADEELHRTGTRLTTQREVE
                     MNLLCSSRLKEALEQAGMELTHFGEVKD"
     gene            4391..5410
                     /locus_tag="PLM79_16790"
     CDS             4391..5410
                     /locus_tag="PLM79_16790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOV88015.1"
                     /translation="MNVKDFLESLSRKIPGRIRPDNPRISVVVPLHNEAETLPELYSL
                     IKDTLEPREDPWEMVLIDDGSTDDTLEILKMLHERDPRVVVVQLRRNYGQTPALMAGF
                     DHARGEIIVAMDGDLQHDPREIPNFLAKIEEGYDLVSGWRTSRSDALLTRKVPSRVAN
                     WLMARISGIELHDFGTTFKAYRREILQDLHLYGELHRFVPALSAWHGARMVEIPIRDL
                     GRNRGSSHYGISRTFRVMFDLVTVGFLLRYMTRPLHFFGKIFLACCSLSGIIGLFLAY
                     RKFFAGVHLFQEHGPLSLLAAALMLAGVQFLAIGLLGEILVRIYFESQDKRIYAVRKL
                     YRKEP"
BASE COUNT         1091 a         1784 c         1741 g         1141 t
ORIGIN      
        1 cgtctgcttc atgttgggga ccttggccag catcgagcgc agcaggggga tcttggttcc
       61 ttggttttgg ggaagaaggg tcaggatgct ctcgacctcg gaaaccaccg gcaaggcctc
      121 caacgaagag gacttggtac gaacctcctc cagggattcg gcaaataccg caccgtataa
      181 gggggaacgt cgggaatccc ggatcagctt cttttcccag accacggctt ccgcccgggg
      241 agcctggagt tttagaggat tcaggtcaaa ccggacccgg gatgcgcctg ccaaaccgag
      301 aaggcagaga accccggcgc agacgaggat caggacggcg gtggggcggc caaaggttag
      361 gagatccccg ttcctggaaa gaaattccga cggagcaaaa tcccaccgct tttccacaaa
      421 aagaacggtc aaggcaggca acagggtaaa atccgccagg agaatcagca ggattcccgc
      481 ccccgtaatg attcccagtt ccatgagccc tcggaagccc gtgaggacca ggggaaggaa
      541 ggaacacgca gcgctgaggc ccgccagcag aatcccgggg ccggaacggt ccgcaaccct
      601 tcgaatcacg gccttccgat ccagccccac gtgacgctct tcctcctcgt accgggcgaa
      661 ccagtggatc ccgtaatcca ctcccaaacc gcacagcaaa ggggcaaaga ccacggacag
      721 gatgttcagg tggccgatga ccagggtggc gaaccccagg gtccagcaga ttcctacccc
      781 cagggaaaag acttcgaagg ccgatcggcg cagacttcga aaggtgagga gcaagatagt
      841 caggacgccc aggagcgaaa gccaggtggc ccgggtcatg tccccgagga ccgtggtcat
      901 ctggtcgttg ttcagggctg cctgccccgt caccccggcc cgcacctctg gaaagttctc
      961 ccgggtttcc cggatgaggg acctcaatcg gtccagggac ctctgtgctc tgctgaaccc
     1021 ctccttttcc ttctcgggaa ccacgaagag gaggagatac ttttttccgg cttcccagaa
     1081 gtagccttcg agatccaggt tccaggagga aggagtgaag aaagagtccc acggggacgt
     1141 aaagtggggg gtccccgaca aataacccga aagcccgctc agggtttgga tcaggaacct
     1201 caggtccaag ggtccttttt cttcgtgccg atcgtcggca agttccggac cgtccaggaa
     1261 gccggtaaac aactccccga ccatgcggga ggccatttcc tggttgacca gccttagaaa
     1321 gatcaggagg tccggttgcc gggtgatccc ctcgatgagg ccggccgaat cgtccagctt
     1381 gtcccgcaaa tggatgagat ccaggggttc cgagtacagg agggcccact gcttcatggt
     1441 ttcggggtcc acccggtaga gtacgtcctc gaagtgagtc ctgtcccttt ggatcttggg
     1501 gaccagttcc tgcacgaaag caacggcgtc ctcgggcttg ggcgcctcca acaccagcac
     1561 caggacggtt ttttctccga agttgaacgg ttcgagtcga tcggcgaggg cgatgagcgg
     1621 gtgatcgggg gagatcagct ccagctgatc ggtctggatc tcgagcctct gggacgccaa
     1681 gattagggag gccaaggccg caaccagggc cgcacccagg acgaaccgcg gcctgggcag
     1741 cacccaggcc gtccaggctt gaagaaggcg ggcgagaaaa ctcatgggga agcgtcagtc
     1801 ctttgccgga tccgattcct catggatttc gggtccggat ctagtatcgc tgggatcgat
     1861 actccccgac ggcgtagctg agcttggcca gggacacgtg gtagttgtaa agggaaagga
     1921 ggtattcgcc ctggttcttc ccgtaacggt cgatggcgtc gaagatgtcc ttggcgggtc
     1981 ctacccccat gtcgaagttg gagaaggccg aaacgatcca cttgcgggcg gcaacggcgg
     2041 ccttttcgta ggcctggaaa gaagcctggg actcgatgac gtcctggtaa tacttggcaa
     2101 cctccagggg gatgttctgc tcggcgtatt cttgggtgtg gagcatcttc tggtgttccg
     2161 cccgggcctt gcggactcga gcctgtccga tccctaggtc gaagtgccaa ttggctccca
     2221 gaatcgctcc catgtaggcg tgattgaatt cgtcctcgat gtaggaatcg gccatccgtt
     2281 cccggccggg cgccccggca aaggagccga cggccgcggc gaagacggaa ggataaagat
     2341 cggccttggc ggcttccacc aaagcccggc gagcttcgat cccccgccgg atttgttcga
     2401 attcgggcct ctggctcaag gctttccgag tgtactcctc ctgggatccc agggccctcg
     2461 tgtccttggg aagggttttc ctatccagct caaaatcctt gtcctcgggg tatccgatgt
     2521 gtttcttgag ggccaggtag gccatctggg ctcccgtttg ggccttgacc ttgaactgtt
     2581 ggatttccgc ttcgaaggct tccaacttgt atagatcgct ctcatccgcg ttcttggcct
     2641 tgagctcgat caaccgcttg attcgcctgc gagcgtcctg gatgaaggtg tccgcgtcgt
     2701 cggcggcctc aatgccctgc ctggccacga tatagctgaa gtagtattcc ttggccgtca
     2761 ggatgacttc cccccgtttc ttttccatgg cggctttttg agcctccagg ccgtgagctg
     2821 cggcgtcccg gcggttggaa atcttgccga aggtataaag aggttgaaca atggacagtt
     2881 cgagccttcc gaagaatcca atgctggtct catcccggtt cttgatctgg cctcggagaa
     2941 atccctcggc gtccttggag ggagtcacaa gcacaacggg tgtccgggca tcctgcaccg
     3001 gtcccccgaa ggccgtgatg tccatctggg cccattgccc cgccttggct tgggccagat
     3061 cggtctcggc cgccttcaca tcctgttccg cttcccggat ctgagggctg tactccacgg
     3121 ccatgccaat gacctggtcc agggtcagga ccggcttttc cgacgccggg ctttcctcgg
     3181 cggcgaaaac ccccaggcaa aacagaaaca ggatcatcca acgaccctta acaccccggc
     3241 gagtccttct catgtcaatc cctcccgcat tgtggttctt tctacagaca tcgtggcacg
     3301 atacagtagt tcgcagggat tgaaaaggct tatcggccgc cgggcgcgcc cgtcccttga
     3361 caccccccgg tggttttgtt agcaatgcat cgtcagccgc gggaccggcc ggcccaggga
     3421 ggacccggaa ccggacaggg agcggcaaac cgccaaccac ccagccaccg aggtcttggt
     3481 tgaagaatcc cccgtgttca ggtcccaact cgaggaaacg gcgactcatc gtcaatgccg
     3541 acgacttcgg gtttacccgc ggagtgaacc gagccgtgga gcgatgcttc acccggggca
     3601 cggttcgcag tgccagcctc atggcgggcg gttccgcctt cgaggacgcc gcccggttgg
     3661 ctcggtctct tcccgggctg ggcgtcgggg cgcacctggt cttgacgggt ctcagggccg
     3721 tatccgagcc gggcagcctt cccggactgg cggattcccg agggtttctt ccccccagtc
     3781 ccggcgccct catcgagcgt ctcctgcgtt tcccggacgc ccgggcctcg gttttcaggg
     3841 aactgaatgc ccagttgtcc cggatcgtcg atgcaggcat tcgaccgact cacctggaca
     3901 gccacaagca tgtgcatatc ctgccgcccg tccttcgatc cgtcacggac ctggcccgca
     3961 agtactcggt gcgctggatc cggaaccctt tcgaggtcga gaatgcgggc tcccttctcc
     4021 ggggagtggc tccctcccac agaaaggcct tctggaagca acacgtcaaa agcctcgtcg
     4081 cccgagtttt tcgtcccggg ttcatggggc acgtaggcac caccgggctt cgtctccccg
     4141 accgcttcca cggcgtttcc ctgaccggcc tgtggaacga agaggctgcc ctgcacacca
     4201 tcggacgatt gtccccgggc ctgaacgagt ggatgttcca ccccggagaa gcggatgaag
     4261 agcttcaccg gacagggact cgactgacga cccagcggga agtggaaatg aacctgcttt
     4321 gctcctcccg tcttaaggag gccctggagc aggccggtat ggaactgacc catttcggcg
     4381 aggtgaagga ttgaacgtaa aggatttctt ggaaagcctg tcccggaaga ttcccggcag
     4441 gatccggccg gataaccccc ggatctccgt ggtggttccc cttcacaacg aggccgagac
     4501 cctccccgaa ctgtactcgc tgatcaagga cacgttggag ccccgggaag acccctggga
     4561 gatggttctc atcgatgacg ggagcacgga cgacaccctc gaaatcctca agatgctcca
     4621 cgaaagagac cctcgagtgg tggtggtcca acttcgcaga aactacggcc agacccccgc
     4681 cctcatggcc ggattcgacc atgcccgggg ggagatcatt gtggccatgg acggagacct
     4741 ccagcacgat ccccgggaaa tccccaactt cctggccaag atcgaggagg gatacgatct
     4801 ggtgtccgga tggagaacgt ctcggtcgga tgcgctcctc acccgcaagg tgccctcccg
     4861 ggtggccaat tggctcatgg cccgcatttc cggaatcgag ctccacgact tcggcaccac
     4921 cttcaaggcc tatcggcggg agatcctcca agacctgcac ctctacggag agctgcaccg
     4981 gttcgtcccg gccctttccg cctggcacgg cgcccgcatg gtggagatcc ccatccggga
     5041 cctgggtcgg aatcgagggt cctcccatta cgggatctcc cgtacctttc gagtgatgtt
     5101 cgacctggtg acggtgggtt tcctgcttcg ctacatgacc cggcccctgc attttttcgg
     5161 aaagattttc cttgcctgct gctctctgtc cggcatcatc gggctttttc tggcctaccg
     5221 gaaattcttt gcgggggttc acctgtttca ggaacatggg ccgttgagcc tcctggctgc
     5281 ggccctcatg ttggccgggg tgcagttcct cgccatcgga ctcctgggag aaatcctggt
     5341 gcggatctac ttcgaatccc aggacaagcg gatctatgcc gtgaggaaac tctatcggaa
     5401 ggaaccataa ggttccggag agggctgccc aatccggtgt ccgcaggaac cgccgtgcct
     5461 gccggggtcg gaatccggga gcctctcata gtgcccccgg ggtcaggtct ccgtatggct
     5521 tttcccgtgc atgcagaggg tgtaggggcg accggccgcc cctggcaggc ctgtggggcg
     5581 caatcccact ctcggtcacg ggacggttct gcgaccccat gggccgcctt cctcttgaaa
     5641 cctgttccgg gggtatcggc cctgcggcct cgcccccccg gctcattgca aaccctccgg
     5701 gctcgctcgg aggcggcgat ctatccccgc ctgatcgcag atgtgggtaa agggcag
//
LOCUS       DAOOVC010000207         5722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_7993_length_5722_cov_6.174519, whole genome shotgun sequence.
ACCESSION   DAOOVC010000207 DAOOVC010000000
VERSION     DAOOVC010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7993_length_5722_cov_6.174519"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PLM79_16795"
     CDS             complement(<1..1697)
                     /locus_tag="PLM79_16795"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88016.1"
                     /translation="MNPIAAENERLRERLDRQAAELQRLHGALKESREKFNALLDRTE
                     DVVFICDRHGRILDINRAARHMLGLSPDSTSIESAFDLFAPRDSVHLAQGMEKALESG
                     SCRVEASLLRRDGNRLPVEILSRAVEYPAGTAVLNIARDLSRTRCRESEQVSHSTQLM
                     TLCRLAQDINAALSTDTLARKAMEAVSSCVEADVCLLFLREGGDLVLKGQHPATAVSH
                     AEQGPAHRLGECLCGLAGGEGKPQYSSDIHSDPRCVRNECKEAGFESFAAVPLKSDST
                     ILGVIGLGSRTLRDFAPDAAFLETIANSVAVALRNAVLYEETRESARELQRKLREIEE
                     REQENRRLQDQLAQSQKMEAIGTLAGGIAHDFNNILTSIMGFTELVLYHFPPESPFYE
                     DLNSVLDAAKHAKELIGQILTFSRQDETEYHPLMPGPVIKETLKLLRASLPSTIDIRD
                     SIDPDAGPVLANATKIQQIVLNLCTNAAHAMEDQGGGVLELSLREIPPSPDHKVGPWV
                     EISVKDTGQGIPEEIRDRIFDPYFTTKKKGQGTGLGLAVVHGIVQSCHGHLCVESKTG
                     "
     gene            2308..3594
                     /locus_tag="PLM79_16800"
     CDS             2308..3594
                     /locus_tag="PLM79_16800"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOV88017.1"
                     /translation="MLSSYRESIRYLYGLQKYGIKFGLNCTENLLARLGNPHRAFRSV
                     HVAGTNGKGSTAAMLSSVLKEHGLQVGLYTSPHLVRFTERFRMNDTEVSPERIMRVFG
                     KIYPLLDQRVPPTFFEVVTAMAFAYFAEENADWAVIEVGMGGRLDATNVIKPRVSVVT
                     NISFDHQEFLGSTLSSIAREKAGIIKTGVPVVTGARQPVVQGILKAACLKHKTSLFSL
                     GRDFRVRRNGEGTFQYVGMRRRWSSLKVPLEGPHQTANAALALAALEILERQGDVRLD
                     PAAVQRGLSRVLWPARLEILQDNPLVILDGAHNPQGAESLRDALKNSFSYRHLHLVMG
                     VMADKDIRGIFRRLLPLAETAVFTQPRYARAANPEELRRLARSYIRKHYVIPDPASAI
                     QQAKLLAGPEDLICITGSLYFAGEVKELYGEPTLPE"
     gene            3629..>5722
                     /gene="lptD"
                     /locus_tag="PLM79_16805"
     CDS             3629..>5722
                     /gene="lptD"
                     /locus_tag="PLM79_16805"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOV88018.1"
                     /translation="MAVETFRAKAFRWIFLCLLCWLGGLAGSPTPAAAQTKPPPFPQL
                     SQERIVSSPQLEWKLEASQITYDQNRGTYEAWGGVRLTAENRMMLADWAELNGERRQV
                     ELWGNVYLQYHKDWLRGEHIVWNLDRETGYVDGGLAYFSQNQFYVKGDRISKIGDKEF
                     ELQQGFLTSCDPADSDWKIRYKHLKVEVGGQATARDTSFWVGGVPILYLPYMRLPVQK
                     ERQSGFLLPWGQYSDLNGMEGELPFYWAIRKDMDATFYGRYMEKRGWMSGGEFRIVHE
                     KWGEGVWAVNYLRDQADKDHLRDEGYPYQTSDRYWIRSRHNFALPNDIDGRLDLDLIS
                     DKNFLNEFREGSASFNFSNRIFEDHFGRGFLFDRTSLVRESSLYLDQRRESALLALDV
                     RYWDQLDKTREDVTLQRFPTLYFNMAPSHVPNWPFYYTMENSLTNYWREKGDRGTRLD
                     LYPRLHYPLQWKSYLNFEPSFGVRSTAYYADWEKGFRSEWQERCFPDVHAELSTRVNR
                     VYPLEVGNLVAVQHAFRPEISYEYAPDGYRGFLPHFDRLDGNLSRHDLRYGFTTFLTT
                     KESLKDPLGNPVSDYREIARLKFFQVFHVEKPPESTPDRWFNPDPRLDAVSTEAEGFG
                     EIGMRLDLMPKRYFTLSYDTTVSPDQGDVLQQDVLLTVDSGRGHIARLDYRQRKDVQV
                     DEIIPQLYVNVLPNIF"
BASE COUNT         1117 a         1658 c         1760 g         1187 t
ORIGIN      
        1 cccgtcttgc tctccacgca caggtgcccg tggcagcttt ggacgattcc gtgaaccacg
       61 gccaatccca gacccgtccc ctgtcctttc ttcttggtgg tgaagtaggg atcaaagatc
      121 cggtccctga tttcctcggg aattccctgc cccgtgtcct tgactgaaat ctcgacccaa
      181 gggccgactt tgtggtccgg ggaaggagga atttccctca aactcagttc gaggaccccg
      241 ccgccctggt cctccattgc gtgggcggca ttggtgcaaa gattcagaac gatttgctgg
      301 atcttggtgg cgttggcaag gacgggtccc gcatcggggt cgatggagtc gcgaatgtcg
      361 atggtactgg gaagggaggc ccggaggagt ttgagggttt ccttgatgac gggacccggc
      421 atgagcggat ggtattcggt ctcgtcttgt ctgctgaaag tcaggatctg gccgatgagt
      481 tccttggcgt gcttggcggc gtccagtacg ctgttcaggt cctcgtagaa gggagattcc
      541 ggggggaaat gatagagaac cagttccgta aatcccatga tggaggtcag gatgttgttg
      601 aaatcgtggg caattccccc tgcaagagtg ccgatggcct ccatcttctg agactgggcg
      661 agctggtctt gtagtcgccg attctcctgt tccctctctt caatctcccg gagctttcgt
      721 tgaagctccc gggcgctttc ccgggtttcc tcatagagaa cggcgttcct gagcgctacc
      781 gccacactgt tggcgatggt ttcgaggaag gcggcatccg gggcgaagtc ccggagggta
      841 cgggaaccca ggccgatcac ccccaggatt gtcgaatcac tcttgagagg aacggcggca
      901 aaggactcga aaccggcctc cttgcattcg tttcggacgc atcgcggatc gctgtggatg
      961 tcggaggagt attgaggttt tccctcgccg ccggccaggc cgcagagaca ttcccccagg
     1021 cggtgtgccg gaccttgctc cgcgtgggag acggcggttg cgggatgttg acctttgagg
     1081 accaggtcgc cgccttcccg gaggaacaac aggcacacgt ccgcctccac gcaggaagac
     1141 acggcttcca tcgcctttcg tgcgagggtg tcggtggaca gggcggcatt gatgtcctgg
     1201 gccagtcggc agagggtcat caactgggtg ctgtggctca cctgttccga ctcccggcat
     1261 cttgttcggg aaagatcccg ggcgatattg aggaccgcag ttcccgcggg gtattccacc
     1321 gcccggctca ggatctccac ggggagccga ttcccgtccc ggcgcaagag ggaggcttcg
     1381 acccggcatg aaccgctttc gagagctttc tccatccctt gcgccaagtg aacggagtcc
     1441 cggggagcga ataggtcgaa tgcggactcg atggacgtgg aatccgggga aaggccgagc
     1501 atgtgccggg cagcccggtt gatatcgagg attctcccat gccggtcgca aatgaaaacg
     1561 acatcctccg tgcggtcgag caaggcattg aatttctccc gggattcctt gagggctcca
     1621 tggaggcgct gaagctccgc cgcctgtcgg tccagtcttt ctcgcaggcg ttcgttttcg
     1681 gcagcaatgg ggttcatgaa cggatcactc gcgatttcct ttgccgggct tgcctccgtc
     1741 gggcggtctc ggatggcgga aggcttccgc cccatcctga aaacaagcac ggatgaaccg
     1801 gggagtcttg tccggggacc ggtccctaca ctcctcccgt ccctcaatcc tgggagggga
     1861 aacgactcct tcgcctccgc cgggagcggg ctgggtcagg ctaggattcc cgaacggacg
     1921 gttactctac ctcaagtccc gccggggtgc aatccgtggt caagagggtc gggatcgccg
     1981 ggttgaggtc tcctcccggg attttccgcg attttccccg aatggaaaag gatcggtgag
     2041 cccgtcgtgg ccgggccggg cgggagggtc gagtttccac gttttttctt accgggaatt
     2101 gtggtatgag ttgccccaga ttccgagcac actttctatc gagcgggtcg aagaagtgtc
     2161 atatcgagcc gaagggaagg catcgttgcc ggcaaggccc gtccattcgg acgagttctc
     2221 ccttcgccgc gccgggttcg aaaggacagg gtggaccatt cggacactct ctgagcactc
     2281 agaaccaaat ggagaggacg gaacagcttg ttgtccagtt accgggagtc aatccgctac
     2341 ctgtacggtt tgcagaaata cgggatcaag ttcggcctga actgcacgga gaatcttctt
     2401 gccaggctgg gaaatcccca tcgtgcgttt cggtccgttc acgtggcggg aaccaacggc
     2461 aaggggtcta cggcggccat gctttccagt gtgttgaagg agcacggtct tcaggtcggc
     2521 ctttacacgt cccctcacct ggttcgcttc accgagcgtt ttcgaatgaa cgacacggaa
     2581 gtctctcccg agcgcatcat gcgggtgttt ggcaagatct atccgctgtt ggaccagagg
     2641 gttccgccga cctttttcga ggtggtcacg gccatggcct tcgcgtattt tgccgaggaa
     2701 aatgccgact gggccgtcat cgaggtgggg atgggcgggc ggttggacgc caccaacgtg
     2761 attaagcccc gggtgagtgt cgtgaccaat atctcttttg atcaccagga atttctcggg
     2821 agcactttgt cttccattgc ccgggaaaag gcggggatca tcaagaccgg ggttcccgta
     2881 gtgacgggag cgcgccagcc ggtggtgcag ggaatcctca aggcggcctg cctgaagcac
     2941 aaaacgtctc tattcagcct ggggcgggat ttccgggtgc gtcgcaatgg agagggaacc
     3001 tttcaatacg tgggaatgag gcgccggtgg tcttccttga aggtccccct cgaggggcct
     3061 caccagaccg ccaatgcggc cttggccctt gccgccctgg aaatcctcga gaggcagggg
     3121 gacgtccgtc tggatcccgc agccgttcag cgggggctct cccgggtcct ttggcccgca
     3181 agattggaga tactgcagga caatcccctg gtcatcctgg acggggccca caacccccaa
     3241 ggcgccgaat cgttgaggga tgcgctgaag aattccttct cctaccgcca tctccacctg
     3301 gtgatgggag tcatggcgga caaggacatc cgagggatat tccgaagact gctgcccctg
     3361 gccgagacgg ccgtttttac ccagccgcgc tatgctcgtg cggcaaatcc cgaagaactg
     3421 aggcgtctgg ccaggtcgta catccgaaaa cactacgtca ttccggaccc ggcatccgcc
     3481 atccagcagg ccaagctcct ggcaggcccc gaggacctca tctgcatcac cgggtccctg
     3541 tactttgcgg gagaagtcaa ggagctgtat ggggaaccga ctctaccgga atgaccgggg
     3601 agcccggggt ccgatctcga ggggatcgat ggcagtagaa acgtttcgtg ccaaggcctt
     3661 ccgatggatc tttctttgcc tcctgtgctg gctgggcggc ttggcggggt cccctacccc
     3721 ggccgcggcg cagacaaagc cgcctccctt cccccagttg agtcaggagc gcatcgtcag
     3781 ttcccctcag ctggaatgga aactcgaggc ctcccagatc acctacgacc agaaccgggg
     3841 cacctatgaa gcctggggag gggtgaggct caccgccgaa aaccgcatga tgctggcgga
     3901 ctgggccgag ctcaacggag aaagaagaca ggtggagctg tggggaaacg tctacctcca
     3961 gtaccacaag gactggctcc ggggcgaaca catcgtgtgg aacctggacc gggagacggg
     4021 gtacgtggac ggaggccttg cctatttttc ccagaaccag ttctacgtga aaggggaccg
     4081 catctccaag atcggggaca aggaattcga attgcagcag gggttcctca ccagttgcga
     4141 tcccgccgat tcggactgga aaattcgcta taaacacctg aaggtggagg tgggaggaca
     4201 ggccacggcc cgggatacct ccttctgggt gggaggcgtt cccatcctgt atctgcccta
     4261 catgcggctg ccggtgcaaa aggaacggca gtcgggcttc ctgctcccct ggggtcagta
     4321 ctcggacctg aacgggatgg aaggcgagct tcccttctac tgggccatcc gcaaggacat
     4381 ggatgccacc ttctacggca ggtacatgga aaaacgtggg tggatgagcg gaggggaatt
     4441 tcgaatcgtt cacgagaaat ggggtgaagg ggtctgggcc gtcaattacc tcagggacca
     4501 agcggataag gatcacttgc gggacgaggg gtacccttac caaaccagcg accggtactg
     4561 gattcgatca cgccacaatt ttgcgctccc caacgacatc gatggacggc tggatctcga
     4621 cctgatcagc gacaagaact ttttgaacga attccgagag gggtcggcgt ccttcaactt
     4681 ttccaaccgg atctttgaag accacttcgg caggggtttt ctattcgatc ggaccagctt
     4741 ggtgagagaa tcgagcctgt acctggatca gcgtcgtgaa tcggccctct tggccctgga
     4801 cgtgcgctac tgggatcaac tggacaagac cagggaagat gtgaccctcc aacggtttcc
     4861 gaccctttac ttcaatatgg ctcccagcca tgtccccaat tggcccttct actacaccat
     4921 ggaaaactcc ctgaccaatt actggcgaga gaagggagac cggggaaccc gcctggacct
     4981 ctacccccgg ctgcactatc cattgcagtg gaaaagttac ctgaatttcg aaccttcctt
     5041 tggcgttcga tccacggcgt actacgccga ttgggaaaaa ggttttcgga gcgaatggca
     5101 ggagcggtgc tttcccgatg tgcacgcgga attgagcacc cgcgtgaacc gggtgtatcc
     5161 cctcgaggtc ggcaaccttg tggccgtgca gcacgctttc cggcctgaga tttcctacga
     5221 gtacgctccg gacggatacc ggggctttct ccctcacttc gaccggttgg acgggaacct
     5281 gagccgtcat gaccttcgct atgggttcac cacctttctc accactaagg agtcgttgaa
     5341 ggaccccctg ggaaatcccg tttcggacta ccgggagatt gcgcgcctga agttttttca
     5401 ggtgtttcac gtggaaaagc cccccgagtc gaccccggac cgctggttca atcccgaccc
     5461 tcgattggat gccgtatcca cggaagccga agggttcgga gaaatcggca tgcgcctgga
     5521 cctcatgccc aagcgctact tcaccctatc ctacgacacc accgtttccc ccgaccaagg
     5581 agacgtgctg caacaggacg tcctgctgac ggtggacagc gggcgcggcc acattgcccg
     5641 gctggactac cgccagcgca aggatgtgca ggtggacgag atcatccccc agctatacgt
     5701 gaatgtcctg cccaacatct tc
//
LOCUS       DAOOVC010000208         5711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8011_length_5711_cov_4.667786, whole genome shotgun sequence.
ACCESSION   DAOOVC010000208 DAOOVC010000000
VERSION     DAOOVC010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8011_length_5711_cov_4.667786"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1836)
                     /locus_tag="PLM79_16810"
     CDS             complement(4..1836)
                     /locus_tag="PLM79_16810"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOV88019.1"
                     /translation="MVLQVPERKRRGTTLDHRQVQGRAFRSLDRRHQGPDGNVGVARR
                     VPGGSEGGRQGIERNSNRELTEQVRISGGLSYINIANAQGEVRASSIPGSIGKVKIGD
                     REYFRKAMKGEVNVSNVYLARTTGKPAFAVAAPIRDEGGKIVGILAGVPDLGKFSQQF
                     VDPVKVLRSGYLAVTDSTGVVIAHKDESLIFKMNLNDQDFGREMLRLKRGHLTYEFQG
                     QKKVAFLEQCGQVDWMISVVAPHKEVVEDAHRMALMNVAILFCGLGLVILVLFFIAHS
                     ITRPINRVVEGLSGGAEQVAAAASHISAASQHLAEGASEQAASIQETSSSLEQMSSMT
                     RQNAHNADLARSATAEAFRVIQEAHGSMDELRKSMGEISVASEETQKIVKTIDEIAFQ
                     TNLLALNAAVEAARAGEAGAGFAVVADEVRNLALRAADAAKNTDALIEDTVRKVKDGT
                     HIAAKARDVFDKVASSSGKVGELVGEIAAASREQAQGIDQINKAVAEMDKVVQQNAAN
                     AEESASSSEEMNAQAEQLRAFVRDLEIIVEGEVDGNHKKKPRPPDGAGDRPGPVMSAV
                     RTFLRSETGASLPGDTTHGEEQTKNPMLLLHQEPSPRGRDTRDF"
     gene            complement(1752..1928)
                     /locus_tag="PLM79_16815"
     CDS             complement(1752..1928)
                     /locus_tag="PLM79_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88020.1"
                     /translation="MMKFGLKKKILVPTSILIISMMGASTGINYLWSSKSLNENAVEQ
                     LSTIARSKAELFDL"
     gene            complement(2279..>3556)
                     /locus_tag="PLM79_16820"
     CDS             complement(2279..>3556)
                     /locus_tag="PLM79_16820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOV88021.1"
                     /translation="RSPQDPLRPVMDLLRGEAAELVCSRYGISPGDLEQRLRAYQESR
                     RRLALEDHLTLQRVGRNAPCPCGSGKKFKKCCLPHHEELRRTLPQDRLKEMEDRSRQQ
                     EQLDKEIEKGFDHLHTGSFQKARRIAETLLETFPEHDRLHDILVFCDLAGERYEEAFL
                     RCRRRWQVSLEEKAFYQENGFHKREGVERKHLVHFYSPSTWLEKFWLAQRAMSYAEAY
                     PRVEDARLSAMVEQLRTANDLQRFPQRGEEGFEARKQALAPILGELEIEGPRTLPYLL
                     PLTYNFSWAALFVPDLLKAFGTEDCIRLLGELSMFRFPFFAQRCLQHLESFGERAVAS
                     IEILWEENPAFDELKVGTITVLGNLQVPSSYELLTRLIDHENTYVVRWALEALSRHGR
                     PEAQPSLERARHRLAAEDRIRSLVNELAAGREK"
     assembly_gap    3557..3656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4739..5200
                     /gene="nuoE"
                     /locus_tag="PLM79_16825"
     CDS             4739..5200
                     /gene="nuoE"
                     /locus_tag="PLM79_16825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOV88022.1"
                     /translation="MKKNLQEIFSNHPRQRDALIPILQDIQGIYGYLPAETMKAAARH
                     CRIDPVEVYGVATFYAQFKFRPIGLNTVMVCLGTACHVMGGARILEEVQSQLGVRPGE
                     STEDGVFTLETVACIGACALAPAMVVNKDTHGRMKAEKVTEILNAARNNSN"
     gene            5175..>5711
                     /locus_tag="PLM79_16830"
     CDS             5175..>5711
                     /locus_tag="PLM79_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV88023.1"
                     /translation="MQPETTAIRIPEGKERLLLVCRGTGCESQKAKAIYRGLLDGLRE
                     AGLAESVAVKFTGCHGLCQMGPTVLVEPEGTFYCTVKPEDVPDIINLDLMKGGKVERL
                     LFFDSKSKTNILSYRDMKFFGPQRRIVLRNCGFIDPEDIDDYIAVGGYEAIRKVFGMS
                     QMDVINEVKKSGLRGRGGG"
BASE COUNT         1081 a         1637 c         1721 g         1172 t
ORIGIN      
        1 ttgctagaaa tccctcgtgt cccggccgcg aggggacggc tcctggtgga ggaggagcat
       61 tgggttcttg gtttgttcct cgccgtgggt tgtatcaccg gggagggaag ctccggtttc
      121 ggatcgcaag aaggttcgga cggcggacat gaccggaccc ggccgatcgc ctgcgccatc
      181 cgggggcctt ggcttcttct tgtgattgcc gtcaacctct ccttccacaa tgatctccaa
      241 atcccgaacg aaagctcgca gttgttccgc ctgggcgttc atctcttccg aggacgaagc
      301 ggattcctcg gcattggcgg cattttgctg cacgaccttg tccatttcgg caacggcttt
      361 gttgatctgg tcgatcccct gggcctgctc acgggatgcg gctgcaattt cccccaccaa
      421 ctccccgacc ttgcccgagg acgacgccac cttgtcgaag acatcccggg ccttggcggc
      481 aatgtgggtc ccgtctttga cctttctcac cgtgtcctcg atgagggcgt ccgtgttctt
      541 ggcggcatcg gcggcccgca gggccaggtt ccggacctcg tccgccacaa cggcgaatcc
      601 ggcacccgcc tcccctgccc gggcagcttc caccgcggcg ttgagggcaa ggagattggt
      661 ctgaaaggcg atttcgtcaa tggtcttgac gatcttctgg gtttcctcgc tggcgacgga
      721 aatctctccc atggacttgc gcagttcgtc catggaaccg tgggcctctt ggatcacccg
      781 gaaggcctcg gccgtagcgg aacgggcgag atcggcgttg tgggcgttct gccgggtcat
      841 ggaagacatc tgctccaggg aggaggaagt ctcctggatg gaggccgcct gctcggaagc
      901 cccctcggcc aaatgctgac tggccgccga gatgtgggaa gccgcggcgg ccacttgctc
      961 cgctcccccg ctcagtcctt ccaccacgcg gttgatggga cgggtgatgg aatgagcgat
     1021 gaaaaacagc acaaggatca cgagtcccag gccgcagaaa aggatcgcca cattcatgag
     1081 cgccatgcgg tgagcgtcct ccaccacttc cttgtggggg gccacgacgc tgatcatcca
     1141 gtccacctgg ccgcactgct cgagaaacgc cactttcttc tggccttgga attcataggt
     1201 gagatgtccc cgtttgagtc tgagcatctc ccggccgaaa tcctggtcgt tgaggttcat
     1261 tttaaaaatg agggactcgt ccttgtgggc gatcaccact cccgtggaat ccgtgacggc
     1321 gaggtagccc gaacgtagga ccttgacggg gtccacgaac tgttgactga attttcccag
     1381 gtcgggcacc cccgcaagga tcccgacgat ctttcctccc tcatcgcgga tgggtgccgc
     1441 cacggcaaag gcgggcttgc cggtggttct ggcaagatag acgttggaaa cgttgacctc
     1501 acccttcatc gccttccgga aatactcccg atccccgatc ttcacctttc cgatggatcc
     1561 cgggatggaa gaggcccgga cttccccctg ggcattggcg atgttgatat aggacagccc
     1621 gccggagatc ctcacctgct ccgtcagttc ccgattgctg tttctctcga ttccttgtcg
     1681 tccgccttca gaaccgccag gtacacgcct cgcgacgccg acgtttccat cagggccttg
     1741 atgtcgccga tctagagatc gaaaagctcg gccttggacc tggcgatggt cgagagttgt
     1801 tccacggcgt tttcgttcag ggacttggag gaccagagat agttgatccc ggtggatgcg
     1861 cccatcatcg agatgatcaa gatgctggtg ggaaccagga tcttcttctt gagaccgaat
     1921 ttcatcattg ttctttcctt tctcgaagca cgtacttgtc atccggagcc gccgtcgggg
     1981 tttcagggtg ctgggagcgg tttgcccggg cgggtcccct tcttgcctgg gtgtccgggg
     2041 tcttcggact tgcggtcacg gaaattgcaa tgatcgcgcc attctcgatc ccggtaggga
     2101 gtcattctgc tttgcaccgg tacatcaaga ggatcgatgg ggaggtgcgg acaccggacg
     2161 ggagggaaaa ccccttgggg tcccccccgc gacgccgggg atgcaaggat ccggacgggg
     2221 gcgaggcggt attttgacgt tcccgctgga agaacggcaa ggcaagggaa taagacggct
     2281 acttctcccg gccggcagcc aattcattca ccagggaacg gatccggtcc tcggcggcga
     2341 gccggtgccg ggccctctcg agggatggct gtgcctcggg gcgtccatga cggctcaagg
     2401 cttccagggc ccaacgcacc acgtacgtat tctcatggtc gatgagccgt gtgaggagct
     2461 catagctcga aggcacctga aggttcccca ggaccgtgat ggtaccgact ttgagctcgt
     2521 cgaaggccgg gttttcctcc caaaggattt caatggaagc aacggcccgc tccccgaagg
     2581 actccaggtg ctgcaggcat cgctgggcga agaagggaaa ccggaacatg gaaagttctc
     2641 ccaggagccg aatgcagtct tcggtgccga aagccttgag caggtccggt acaaaaaggg
     2701 ccgcccacga gaagttgtac gtcaggggca gaaggtaggg aagggttcgg ggaccttcga
     2761 tttccagttc tccgaggatg ggggccaggg cttgcttcct ggcctcgaag ccttcctctc
     2821 ccctttgggg aaagcgctgc aggtcgttgg ccgtgcgcag ttgttccacc atggccgaca
     2881 ggcgggcgtc ttccacccgc gggtaggctt cggcgtagga catggccctt tgggcgagcc
     2941 agaacttttc caaccaggtg gacggagaat agaagtgaac gagatgtttc cgttccactc
     3001 cctcccgctt gtggaacccg ttttcctggt agaacgcctt ctcctcgagg gaaacctgcc
     3061 agcggcgtcg gcacctcaga aaggcttctt cgtagcgttc tccggccaag tcgcagaaaa
     3121 ccaggatatc gtggagacgg tcgtgttcgg gaaaggtctc caagagcgtc tccgcgatcc
     3181 ttcgggcctt ttgaaagctt ccggtgtgca ggtgatcgaa gcctttctcg atttccttgt
     3241 ccaactgttc ctgttgccgg gagcggtcct ccatttcttt gagccggtcc tggggcaggg
     3301 tgcgccgcag ttcttcgtgg tggggaagac agcacttctt gaactttttc ccggacccgc
     3361 agggacaagg ggcgttgcgt cccacccgct gcagagtgag gtggtcttcc agggccagcc
     3421 gtcttcgaga ctcctggtag gctctcaggc gctgttccag gtcgccgggc gaaatgccgt
     3481 accggctgca gacgagttcg gccgcttctc ctcgaagcag gtccatcacg ggccgcaaag
     3541 ggtcttgggg agaacgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgc
     3661 ttctcctcga agcaggtcca tcacgggccg caaagggtct tggggagaac ggtccgcact
     3721 tcccgggcga aggttttccg ccatcgctac atcctcttct ttctgaatcg ataggcgcct
     3781 ggaaacggcg catgcgggtg ttgggaccct gccgccggga gggctcacgc cttccggaaa
     3841 ccggaaatcc ccctgcctta tattcaactc cccgggtttt ggccagtgtt atgtgacgat
     3901 tttccgggag aaatcctctt ggtgcggggc gggaaagtcg acggacggcg cgcccggggg
     3961 gcaaaaccga cgggatccgc gaaaaataag gaccgcagtg gggggccttg ccgccgttcg
     4021 ttccctaggg gactaaaggc aggtttcgtg ccggggcggg tccatcgaag tacaaggcga
     4081 cgggttaaaa gaaatcaaaa aagattccca cagaacaaac ttcttcttga cctgtatcaa
     4141 tatgctaaat taaactggtt tcgcgttgaa tcacggttcc cctgcctatg tggttcacca
     4201 taatccgcgt cggccgtcat gacttccggg ggcgcgaggg tgggttccag tctgccgttg
     4261 acccgatccg cctcccgggg gaagttgcct tcccaggctc atgaccgtcc gggcgggaaa
     4321 catccaggag agcaagcgaa gctaaagttg cgtcggtaag gtcaagtcga aaccggtctg
     4381 ttatttcaac ctccagctgt tagcgcgtcg ggtttcgcac tagcttgaat caggatgttg
     4441 cgggatgaac aacgctatga cgggggagag ccctgtacat accggggaat tcctcacctc
     4501 ccggcttcct tcccttcggg ccgccatcac cctctctgtc ttgacctttg accgtgcaac
     4561 cgttccatgg gccgccttca tgcgtcaatg gtgtggcccg ggctgaccag gcctttgccc
     4621 gggtccgatt cacacacggg gtcttcgacg agcggccgct tgcggccgcg cgaaggagtg
     4681 aaaggacaaa ggacacacac agaacccccc acccctaacg atcgaggaaa attgagtgat
     4741 gaaaaagaac ctgcaagaaa tcttttccaa tcatccacgc cagcgagacg ccctcatccc
     4801 gatcctgcag gacatccagg ggatatacgg ctacctgcct gcggagacga tgaaggctgc
     4861 agcgagacac tgccgcattg accccgtgga ggtctacggc gtggcaacct tttacgccca
     4921 gttcaagttc aggcccatcg gtctgaacac ggtgatggtg tgtctgggaa cggcatgcca
     4981 tgtcatgggc ggcgcccgca tcctggagga ggttcagtcc caactggggg tccgcccggg
     5041 agagagtacg gaagacggcg tcttcaccct cgagacggtg gcatgcatcg gggcgtgtgc
     5101 tctggcgccg gccatggtgg tgaacaagga tacccacggc agaatgaaag ccgaaaaagt
     5161 gacggagata ctgaatgcag ccagaaacaa cagcaattag gatcccggag gggaaggaac
     5221 gtctgctgct cgtttgtcgg gggaccggct gcgagtctca gaaagccaag gccatctacc
     5281 gaggattgct cgacggcttg cgcgaggccg gcctggctga gagcgtcgcg gtaaaattca
     5341 ccgggtgtca cggactgtgc cagatggggc ctacggtatt ggtcgagccc gagggcactt
     5401 tctactgtac ggtcaaaccc gaggacgttc ccgacatcat caacctggac ctcatgaagg
     5461 gcggcaaggt agaacgcctc ctcttcttcg actccaagtc caaaacgaac attctcagct
     5521 acagggacat gaagtttttc ggtcctcaac gccggatcgt ccttcgaaac tgcgggttta
     5581 tcgatcccga ggacatcgac gactacatcg ccgtgggcgg ctatgaagcc attcgaaagg
     5641 tcttcggcat gtcccagatg gacgtcatca atgaggtgaa aaaatccggg cttcgaggcc
     5701 ggggaggcgg a
//
LOCUS       DAOOVC010000209         5698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8032_length_5698_cov_4.212298, whole genome shotgun sequence.
ACCESSION   DAOOVC010000209 DAOOVC010000000
VERSION     DAOOVC010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8032_length_5698_cov_4.212298"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="PLM79_16835"
     CDS             complement(<1..908)
                     /locus_tag="PLM79_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOV88024.1"
                     /translation="MSGQADSFEVTQGRHGPIWKIAKGVRVYKSPDGTFEIEVPEKGK
                     RKRRRVEELEQAIEAAKLFALRAGLPLCTKEGKDLPQGPYLFEHAAWNWFALNKGSWT
                     AATIEKYQGLLRNHILPAIGNLPLLEPYERWRERVKKFLVDLREIRSAKMVEVAHAVI
                     SGVFSEAIDSAKVQINPAQGLLKRILPPKKRRVLSKPDPFSKSDLRAVLDSAWKVLSP
                     DRAMAIEVMAKTGMRVGECLAMHIDNLDIRNRLYWIKEKVRHGKWGVPKDGEDRQVDI
                     SEELMTQLQAHLKRLKERMLSEGKPVG"
     gene            1414..2016
                     /locus_tag="PLM79_16840"
     CDS             1414..2016
                     /locus_tag="PLM79_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88025.1"
                     /translation="MREPEPLYRYFVKFHFEGREEPVIYEVGKATTEQISNNLEGTPE
                     LFCWFPTVRGREVAVNLRAIDLVHFLWEPVAGTSTKEEGESIEDDLDIVIYFRNRKEP
                     FISGAAYPVEVSFIFDSLQLGGFHEDPFLWFSDEDDELVVIDARKLLCIEAPSEMIRE
                     GQEEMNKEASELMESMEEPNLQEPIPERTRKSPIPFRRRK"
     gene            complement(2281..2805)
                     /locus_tag="PLM79_16845"
     CDS             complement(2281..2805)
                     /locus_tag="PLM79_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88026.1"
                     /translation="MSDRGLLLCLRVRGLQEMQDFVNECRERNDMVQEPYGYYYLHEN
                     GDLIWKKLRPEIEPGGPVKKVWPLYPGSRGSQWLVAIEALALGAKKSRIDELSAKWGL
                     TDDDALEFVKHATLKDGTPAMRLFKDGNRWCATFHDFVSIEESPAGFGSTALEALAEL
                     AKPDLLRNISREKP"
     gene            complement(2917..3231)
                     /locus_tag="PLM79_16850"
     CDS             complement(2917..3231)
                     /locus_tag="PLM79_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88027.1"
                     /translation="MHKPTAATKEAYRLIEEWCAKNPGTGSTLGLMRLVLSLYNGKTW
                     SHSIPICIWHLDNTRLQWAKYIILDYLDRGESNELLALAEKFAPMVEEFHWPPDEPQK
                     QE"
     gene            complement(3231..3518)
                     /locus_tag="PLM79_16855"
     CDS             complement(3231..3518)
                     /locus_tag="PLM79_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88028.1"
                     /translation="MKVKRVCDNLMIEFDDIDSDPREILEKIKAFRLLFEREPRLMAN
                     GHWRRVEHPNMGQLYPATKFEITVSSIGYFCGYGEGAVQVDPTPESFIGVF"
     gene            complement(3538..>5698)
                     /locus_tag="PLM79_16860"
     CDS             complement(3538..>5698)
                     /locus_tag="PLM79_16860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOV88029.1"
                     /translation="AVTRGKMNEIVVTGHFYRIGKTDRIRAIGAWQQHPRFGLQFVTT
                     SCEILPPVTEEGIRGFLASGMIKGIRDGIARMLVDHFGMKTLEIVRNAPHRLTEAPGI
                     GKERARKIVDSVFEYQEVEKIMVQLHSLGVGSSLAVRIYKHFLKQSGDSASAGEKALQ
                     TIREQPYELTNIWGIGFTTADSIALKLGFRRDDPRRLRAGLIYVLEHATHEGHVCLSR
                     DRLAADAEKILIGHSSTENGTAIGNAMARLEGEHSLRSAIIDGVYFCYLPNLYVAEQS
                     VATGLKAIAAGAVPIAKAEEAPALIARAERNLAIAFAQEQRCAVATALRGGLMVLTGG
                     PGTGKTTITRAIVEVYSGKDKSILLASPTGRASKRLAEATGREALTIHRLLEYDPKQH
                     RFTRNESNPLNADLIIVDESSMLDIVLANHLVKATTPNTTLLLVGDVDQLPSVGPGRV
                     LQNIINSGLATTVMLKEIFRQAQGSLIVRNAHLVNKGEMPLTRNPSGDPPDFFFLTEP
                     DPEKVRETIVGLVKTRLPAKYSYNPTVDIQILTPMRKGPIGVHALNAALQEALNPPGP
                     PELRSFETTYRTCDKVMQIRNNYEKEVFNGDIGIVTGIDTEENTLFVTYPGIGTVGYE
                     ESELGELVLAYATSIHKSQGSEYPVVVVPVHTQHAIMLQRNLIYTAITRGKKFVVLVG
                     TKQALAMAIKNNNAPERFSHLTHLLREMTDEATRHRR"
BASE COUNT         1120 a         1504 c         1628 g         1446 t
ORIGIN      
        1 ccaaccggtt taccctcgga aagcattcgt tccttgagtc gcttcaggtg ggcttgcagt
       61 tgggtcatca gttcctctga gatgtcaact tgcctgtcct ctccatcttt tggtactccc
      121 catttcccgt gcctgacctt ctccttgatc cagtagagcc ggtttcgaat gtccaggtta
      181 tcgatatgca tcgcaagaca ttctccaacc cgcattccgg tcttggccat cacttcgatt
      241 gccatggccc ggtccggact cagtactttc caggctgaat ccagtaccgc tcgaagatcg
      301 gacttggaaa aggggtccgg tttgctcagg acccttctct ttttgggggg aagaattcgc
      361 ttgagcaacc cttgtgcggg attgatctgc acctttgcgc tgtcgatcgc ttcggagaag
      421 actccggaga tcaccgcatg ggctacctca accatctttg ccgacctgat ctcccgcaga
      481 tccaccagga atttcttgac cctctccctc cacctctcgt agggctcaag gagaggaaga
      541 ttcccaatcg ccggtaagat gtggtttctg agaagccctt gatacttctc aatggtagct
      601 gccgtccagc tacccttatt cagagcaaac cagttccagg cggcatgctc gaatagatac
      661 gggccttgag gcaaatcctt cccttctttc gtgcaaagag gaagccctgc cctcaaggca
      721 aagagcttgg cggcttctat ggcctgctca agttcttcga ctcgtctgcg ttttcgtttt
      781 cccttttctg gaacctcaat ttcaaaagtt ccatccgggc ttttgtagac tctcaccccc
      841 tttgctatct tccaaatggg tccgtgcctg ccctgtgtta cttcaaagct gtcagcctgc
      901 ccgctcatcg cgatcgcacc tccttgagaa tgccgtcgag cgatagcccg acgcgctcct
      961 cagtggtgcc tccggcccgg aattcccgta cccattcatc gaattcactg cgtttaaacc
     1021 ggacactgcg accaatccta tagaaaggca tacccttctt catccacgat ttcaaggtgt
     1081 ttatgtgaag agaggtatac tgggaaagct gcttaatggt aagatattca gtgtccatga
     1141 acgcttggat gctcctttga ttgcgttttg gcaggacttt tcaatgcaag ggcgcaacca
     1201 gaagcaagag aagccatgat gtcgaagtcg ggaaccactt cctgaatccc tggctcgatc
     1261 tggatgtgtc cctgcagaca gaagagcgtg cctgccgaac tcattgaagc tgcttcatga
     1321 aagtttccac tgtggatgct cgtttgctga actgatgtct gaccatacga tctcaatcag
     1381 tctcagcccc gagtgaaatg aaggaggaaa taggtgagag agcctgagcc gctttaccga
     1441 tattttgtga aattccattt tgaaggcagg gaagagcccg tcatctacga agtgggtaag
     1501 gccacgactg agcagatcag caacaattta gaaggcacgc ccgaactgtt ttgctggttt
     1561 cctaccgtca gggggcggga agttgcggtg aatcttaggg ctattgacct cgttcatttc
     1621 ctctgggaac cagtggccgg aacgagtacg aaagaagagg gcgagagcat cgaggatgac
     1681 ctcgatatcg tgatctactt tcggaatcga aaagagccct tcatctccgg tgcagcttat
     1741 cctgtcgagg taagcttcat attcgacagt ctccagctcg gaggctttca cgaggatcct
     1801 tttctatggt tcagtgacga agacgatgag ctggtggtca tcgatgcaag aaagctgctc
     1861 tgcattgagg ccccttcaga aatgatcaga gaggggcagg aggagatgaa caaagaggcg
     1921 agtgagctta tggaatccat ggaagagcca aacctgcaag agcctatccc tgagcgaacg
     1981 cgcaaaagcc cgattccttt caggcgtagg aagtgattgt gcgggattac ggtgaacgga
     2041 gccgttgaaa gaccatccgg gagttggcac agcggagttc ggacgcctcc tttcgagtgg
     2101 tggtggctgg cgcccccgcc ccctaccccc aagcttgctg ttgctttgcc gcgaatgcaa
     2161 atgggtagca tatttcagat ccgatcagaa cgggcggtga tgatcacctg gctcgaaagt
     2221 gcgggggccg gagttggcgg agaatactgg cgccagcgat gccgctgtgc ttgaggtcgg
     2281 ctatggcttc tcgcggctta tgttccgaag aagatcgggc tttgcgagct ccgcgagggc
     2341 ctccagcgcg gtgctcccga atcccgccgg gctctcttca atgcttacga aatcgtggaa
     2401 cgtggcgcac caacggttgc cgtctttgaa gagacgcatg gccggcgtgc cgtccttgag
     2461 ggtggcgtgc tttacgaatt ccagggcgtc atcatccgtg aggccccact tggcgctgag
     2521 ctcatctatt ctggattttt tggcgccgag tgccagggct tctattgcga cgagccattg
     2581 ggatcctctg gagccggggt agagtggcca gacctttttt accggtccgc ccggttcgat
     2641 ttccggacgg agctttttcc agatgaggtc gccgttttcg tgaagatagt agtagccata
     2701 gggttcttga accatgtcat tcctctctct gcattcattg acgaagtctt gcatctcctg
     2761 gagaccccgg actcggaggc acaggagaag tcctctatcg ctcatgcgtc ttttccactc
     2821 ggtaaatcac gccttgggca cgagtataac tcatgatcca ggctcagttc actgagatgg
     2881 taattgcttt atgcttcttc actcacctta ctcctgttac tcctgctttt gtggctcatc
     2941 aggtggccag tggaattcct cgaccatcgg tgcgaatttt tccgccagtg cgaggagctc
     3001 attgctctct ccgcgatcga ggtaatcgag gatgatgtat ttggcccact gcaggcgagt
     3061 attgtcgagg tgccagatgc agatggggat cgagtgggac catgttttac cgttataaag
     3121 gctcaggacc aggcgcatga ggcccagcgt ggaacctgtt ccggggttct tcgcgcacca
     3181 ctcttctatc agtcgatagg cttcctttgt tgctgctgtt ggcttatgca tcagaatact
     3241 cctatgaagc tctcgggcgt gggatcgacc tggacggcac cttctccata gccgcagaaa
     3301 tagccgatgg agctcactgt tatttcaaac ttcgtggcgg gataaagctg ccccatgttt
     3361 gggtgctcga cccttctcca gtggccgttt gccatgagcc ggggctctct ctcgaagaga
     3421 aggcgaaaag ccttgatctt ttcgaggatc tccctggggt cggaatctat gtcgtcaaat
     3481 tcgatcatca agttgtcgca gactcgtttg actttcatca ttcctccttt caggtcctca
     3541 ccggcggtga cgggtggctt catcggtcat ctcacgtaac aaatgggtca gatgggaaaa
     3601 gcgctcaggg gcattgttgt ttttgatggc cattgcaagg gcctgtttgg tgccaacgag
     3661 cactacaaac ttcttgcccc tggtgatggc cgtgtagatc aaattgcgct ggagcatgat
     3721 ggcgtgttgg gtatggactg gcacgacaac caccgggtac tcgctgccct ggctcttgtg
     3781 aatgcttgtg gcataagcca ggacgagctc tccgagctcc gattcttcgt aacctaccgt
     3841 gccgatgccg ggataagtca caaagagcgt gttctcctcg gtgtcgattc cggtcacgat
     3901 gccgatgtct ccgttgaata cctccttttc atagttgttc cggatctgca tgaccttgtc
     3961 gcaggttcgg taggtcgtct cgaaggagcg gagctctggg gggcctggcg ggttgagagc
     4021 ttcctggagg gcggcgttta gggcatggac gccgatcggt cccttacgca tgggagtgag
     4081 gatctggata tccacggtgg ggttgtagct gtatttggct ggcaggcgag tcttgaccag
     4141 gccgacgata gtctctcgga ccttctccgg atcaggctcg gtgaggaaaa agaaatcggg
     4201 tgggtccccg gaggggtttc gggtgagagg catttctccc ttgtttacca ggtgggcgtt
     4261 tcgcacgatg agagaaccct gcgcctggcg gaagatctcc tttagcatca cagtcgtggc
     4321 gaggccggag ttgatgatgt tctggagcac gcggccgggt ccaaccgatg ggagctggtc
     4381 gacgtctccg acaagaagga gcgtggtgtt tggggtagtg gcttttacca gatgatttgc
     4441 gagcacgatg tcgagcatgg aactctcgtc gactatgatg agatccgcgt tgagtggatt
     4501 cgactcattc cgggtgaagc ggtgctgttt tggatcgtac tccaggaggc gatggatggt
     4561 gagggcctct ctgcctgtag cctcggccag gcgttttgat gctctaccgg tcggggaggc
     4621 aagaaggatg cttttgtctt ttccggagta gacttcgacg atggcgcgcg tgatggtggt
     4681 ttttccggta cccggcccgc ccgtgaggac catcagccca ccccggaggg cggttgcaac
     4741 tgcgcagcgc tgttcctggg caaaggcgat ggcgagattc ctttctgccc tggcaatcag
     4801 cgccggcgcc tcttcggcct ttgcgatcgg gacggcgccg gcggcaatcg ctttcagacc
     4861 ggtcgcaacg gattgctcgg cgacgtacag gtttggcagg taacagaaat agacgccgtc
     4921 aatgattgcg cttctgagtg aatgctcgcc ctcaagtctt gccattgcat tcccgatggc
     4981 tgtcccgttt tcggtggatg aatgtcctat gaggatcttt tctgcatctg cagcgagacg
     5041 gtcccgagac aggcacacat ggccttcatg tgtcgcatgt tcgagcacgt agatgaggcc
     5101 tgcgcgcaat cttcttggat cgtcccgcct gaagccaagc tttaaggcga tgctgtctgc
     5161 tgtcgtgaat ccgatgcccc agatgttggt tagctcgtag ggttgctcac ggatggtctg
     5221 caatgctttt tctccggctg acgcggagtc ccccgattgc ttcaggaaat gtttgtagat
     5281 ccggacggca agagatgagc ccaccccaag ggagtggagc tgcaccatga tcttttcgac
     5341 ttcctggtat tcgaaaacgg agtcgacgat cttcctcgcc cgttctttgc cgatgcctgg
     5401 agcttcggta agtcgatgcg gggcgtttcg gacgatctcg agagtcttca ttccgaagtg
     5461 gtctacgagc atgcgtgcga tgccatcccg gattcccttg atcatgccgc tcgcgaggaa
     5521 gccgcggatt ccttcttcgg tcaccggagg gaggatttca cagctcgttg tgacgaactg
     5581 gagcccaaaa cggggatgct gctgccaggc tccgatggct cttatccgat ctgtctttcc
     5641 aatcctgtag aagtggccgg taactacgat ttcgttcatc ttgccgcgag tcacggct
//
LOCUS       DAOOVC010000210         5670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8078_length_5670_cov_4.354052, whole genome shotgun sequence.
ACCESSION   DAOOVC010000210 DAOOVC010000000
VERSION     DAOOVC010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8078_length_5670_cov_4.354052"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="PLM79_16865"
     CDS             <1..1609
                     /locus_tag="PLM79_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88030.1"
                     /translation="KAAEKPYEIHEYNIETLKKILAEEAGAEPAIIEAKAHCAYFEEY
                     FEKIGAKTIIVEKDYVDHYFLDDFTGHYVCCFKPYSRCCTRLHFFKIELTLEEFEEIL
                     KNPTEKVSPQRMREIYLGFVVVKPLPQTIIGRTCLKTFDSDGGRRHYPITRNYRACLF
                     GWNLEVDSLAFQEQDKVVAACSTSALWAVLQGTGKIFHHHIPSPVEITKRATDLLPPM
                     PPETRHFPSHGLSVSQMAQAIKQLDLMPHFINPEDEQLLRGNAYSYLMGRIPILMTFA
                     LVDYIDGKPKLRHGLHAVAITGYSLGNPEPVALKASGKPSGLLTVASRINKLYVHDDQ
                     VGPFVRMEFPGAGNDRPLPSELERQNLLYTLSTSWPLQNGSEEVRAVPLSLMVPLFPK
                     IRIPYSTILDVIIELDFVIETFRANAKTSFEDRLEWEIHLTTNNEFKSKMDEANHLGW
                     GIRRKTLMKGLPRYIWRAKAKSAGEDVIDLLFDATDIEQSTLLVHAVPYDRDLFEILR
                     RRSKNPESVRNIKVKSIWKALDWFSKQ"
     gene            2763..3023
                     /locus_tag="PLM79_16870"
     CDS             2763..3023
                     /locus_tag="PLM79_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV88031.1"
                     /translation="MEEYLTIKELCERIKYERQTIYNLIHRGIFKLGEHFLKPSPKKI
                     LFRWSAIQAWLGEISHTEDSPPGPYKPDPVVSKAQKASLIRI"
     gene            3117..4433
                     /locus_tag="PLM79_16875"
     CDS             3117..4433
                     /locus_tag="PLM79_16875"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HOV88032.1"
                     /translation="MANARASQGNVVAFPGPRKRREKKRKSGLNHNKEGSVRNVNGRV
                     YVDFIYLDERVRESSGLDWNEKNAKTVREQLDKIIAAISSGTFRFAEVFPESKNRDLF
                     SEKEKEKYGAKPPTPDEVLFGEYANKWLSLLEGSGRVSGRTLREYKSYLNHYLIPFFG
                     EMTFAELNAHSFEKFILWARQRELKKISVCNKSINKYFVPMKMICKQAAIEYKWSTGF
                     EPFFGHERLPEDDVSEKIVPFTIEEQAKLRQELPDHWKPYFDFAFRTGLRPGEQIAIK
                     PEDIDWPRGLLHIRRSITLDEDGKRTEGTTKNKYSRRTIKLTPAMLEALSAQKAVHDQ
                     FGCEYFFCSREGRPIHLSNVRRRVWIPALKRAGLAIREMKQTRHTFATVALSCGENPL
                     WIAKTMGHRNSEMIIKVYSRYVENIRGTEDGSIMDRIYQVNKGKEE"
     gene            4894..>5670
                     /locus_tag="PLM79_16880"
     CDS             4894..>5670
                     /locus_tag="PLM79_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOV88033.1"
                     /translation="MTDSLTIARELEASIKADLVREEFDITHHRQKKKPITLNDLWDK
                     YLPWAKGNKRTWRNDLYLYDAHIGPRFGSKPLADITPFLLERMKNEMKRSRNKQGKPF
                     AAATIKHVLVLVRRLFNLAGKWNLYQGANPVKSVEMPKVDNQVTEFLSDEELSRLLNV
                     LDNWPYDDSAAFVKFALFTGMRRGELFKLTWDAVDFERDMITLAAPKGGKTATLPICR
                     EAMDAIRDLPRTSRFVFPGKGGKQRTDFKGPWGRIRKAAGL"
BASE COUNT         1440 a         1460 c         1551 g         1219 t
ORIGIN      
        1 caaagccgca gagaagcctt atgagattca cgaatacaac atcgaaaccc tgaagaagat
       61 tcttgccgag gaggcaggag ccgaaccggc catcatcgaa gccaaggccc attgtgcata
      121 ctttgaggaa tacttcgaga agataggcgc caagaccatc attgtcgaga aagactacgt
      181 cgatcactat ttcctggatg attttaccgg ccactacgtt tgctgcttca agccctacag
      241 ccgctgttgc acgcggcttc attttttcaa aatcgagctc actctcgaag aattcgagga
      301 aatcctcaag aaccccaccg aaaaggtaag cccgcagagg atgcgagaga tctatctcgg
      361 attcgtcgtg gtgaaacccc ttccgcagac cataatcgga agaacctgcc tcaaaacctt
      421 tgactctgat ggaggaagaa gacactaccc cattaccaga aactataggg catgcctttt
      481 tggctggaac ctcgaggtgg actcgctcgc ctttcaggaa caggacaaag tggtggctgc
      541 ctgctcgacg agcgccctgt gggcagttct gcaggggaca ggcaagattt ttcatcacca
      601 tataccctcg cctgttgaga tcaccaaacg tgcgacggat cttctgccgc cgatgccgcc
      661 cgagacacga cacttcccga gtcacggcct ttccgtctct caaatggctc aggcgataaa
      721 gcaactcgat ctcatgcctc acttcatcaa cccggaagat gagcaactcc tgcgcggcaa
      781 tgcctatagt tatctcatgg gtaggattcc aatcctcatg acttttgccc tggtggacta
      841 cattgacggg aaacccaaac ttcgtcatgg tcttcatgct gtggcgataa caggctacag
      901 ccttgggaat cccgaacctg ttgcattgaa ggcatccggc aagccatcgg gtcttcttac
      961 tgtggcatcg aggattaata agttgtacgt tcacgacgat caggtcggcc ccttcgtgcg
     1021 catggaattt cccggggcag gaaatgatcg tccgttgccg tctgagcttg agcgtcaaaa
     1081 cctgctgtat accctctcca cttcctggcc gctccaaaat ggctcggaag aggtaagggc
     1141 tgttcctctc tcgctcatgg tcccgttgtt tccaaagatc cgcattcctt acagcaccat
     1201 tttggacgtg atcatcgagc tcgattttgt gatcgagacg ttcagggcaa acgcaaagac
     1261 ttccttcgag gatcgactcg agtgggagat ccacctgacg acgaacaatg aattcaaaag
     1321 caagatggat gaggcaaatc atctcggctg ggggatccgc cgcaagacac tcatgaaggg
     1381 attgccgcga tacatctggc gggcaaaggc gaagtccgcg ggagaagatg tcatcgatct
     1441 tctctttgac gcaacggata ttgagcagtc aaccctgctc gtgcatgctg taccatatga
     1501 ccgtgatctt tttgaaattc tcaggcgcag gtcgaaaaac cccgagtctg tgagaaacat
     1561 caaagtcaag tccatatgga aagccctcga ctggttttca aagcagtagg gcatttagag
     1621 ggatcactgc ccagcactac caacactctg tccttgccgg cctctgcgga ggtcctgcca
     1681 atattgggcc gattccatgt gcgttctctc cgaatgctcg gtgaagcgtc ggcatcggct
     1741 ttttcttgca gattcctctt tcctttcttt cctgatttca tttctgcgta gccttccaga
     1801 acgttttctc gtcttccacg cctgaaagct tttgtgaggc ggcatttgtc actgctcacc
     1861 tcgtcttctc tagtttatgt aaaataattg tgctaaaatc agaataaata attgactatt
     1921 cccacttagg tctgtagcat aagagcatcc ggaacagaac gaaagcctga gaccgcttga
     1981 ggtagccggt caggtgcatg ccgcaagatg agggctgcgg aaccttggag tggatgccgg
     2041 cactgacggc tacgacttgg aaagaagcag aaataaaccg gatggaacga aatgagctac
     2101 gtagcctgcc ggaaacaatc tctcctgaac gaagctcctg tcaagcgcaa aaagcgggag
     2161 gtcgcaaggc ttttcatacc cttctcacgt gcacgcctat gtcttcaatg tgatgcgatt
     2221 catcaattcg aggtttgtcc cagatgcagt ttcaccgagt ggatgccttt gcctgttgtc
     2281 atggggtgtg accttctgaa gaggcggcta tcgaccgctg aatagccgga ggggtagcag
     2341 aagaatacgt ggcgaacgat ttctctcctg agaagaaagc gagaggagtg acaatggtga
     2401 ctcaaggaag ccaatggagg gccaacggtt acaggtatcg cgatagggag agctcagaaa
     2461 atccgaaggc gccgccgcct gtcaccggtt ccagatcaat cgaggatgaa gcccgccttg
     2521 cagacctcag ttccaggaag cgcaaatcca ttgaagaatt ctgtccccga tgcatgcgag
     2581 acgttccgga gaacacatcc agcttctgtc ctcactgtgg ctgctgtttg aattgtctat
     2641 tctgacctga cgacgggggc agccaggagt tcaaccggca aggccggatt aatgacaagc
     2701 tctccatgaa atcttcagga tcttgacccg ctgcagggcg gtacacgagc aaggacggcg
     2761 atatggaaga gtatctcaca ataaaggagc tctgtgagcg tatcaagtat gagaggcaga
     2821 ccatctacaa tctcatccac aggggaatct tcaaactggg tgagcatttc ctgaagccat
     2881 cgcctaagaa gatcctcttc agatggtccg caattcaggc ctggcttgga gaaatctctc
     2941 ataccgaaga ttctcctccg gggccgtaca aacctgatcc cgtagtttcg aaggctcaaa
     3001 aggcaagtct gatcaggatt tgaaaaggca tccttccgca gtttgctcaa tttctggtag
     3061 aatggcgttg gtgaagatca gcggaaggag gtgatagtta gaaaggaatt gtggatatgg
     3121 ccaatgcgag agcatcacaa ggcaatgtgg tggcttttcc aggcccgagg aaacggcggg
     3181 agaagaagcg gaagtccggc ctcaatcaca acaaggaggg ctccgtccgg aatgtgaacg
     3241 gcagggtcta tgtggacttc atctacctgg atgagcgggt gagggagtct tcggggctcg
     3301 actggaacga gaagaatgcc aagactgtcc gggagcagct cgacaagatc atagcggcca
     3361 tcagttccgg cacgttcagg tttgcagagg tcttcccgga gagcaagaac cgtgacttat
     3421 tctctgaaaa ggagaaagag aaatacggtg ccaagcctcc taccccagat gaagtccttt
     3481 ttggcgaata cgcgaacaag tggctttctc tgctggaagg ttcggggagg gtttccggcc
     3541 gcacactccg agagtacaag agctacctga atcactactt gatcccgttc tttggggaga
     3601 tgacatttgc ggagctcaat gcccactcgt tcgagaaatt cattttgtgg gcacgtcaaa
     3661 gggagttgaa gaagatttcg gtctgcaaca agtcgatcaa caagtatttc gtgcccatga
     3721 agatgatttg caagcaggcg gccattgagt acaagtggag caccggcttc gagcccttct
     3781 ttgggcatga gcgactgccg gaagatgatg tgtcggaaaa gatcgtaccg ttcaccatcg
     3841 aggagcaggc aaagcttcgg caggagttgc cggaccactg gaagccgtac ttcgactttg
     3901 cattccgaac cggccttcgg cctggcgagc agatcgcgat caaaccagaa gacattgact
     3961 ggccaagagg gctcctgcac attcgccgtt ccatcactct cgatgaggac ggcaagcgaa
     4021 cggaaggaac cacgaagaac aagtacagca gaagaacgat caagctgacg ccggcgatgc
     4081 tggaagcact cagcgcccag aaggcggtgc acgatcagtt cggctgcgag tacttcttct
     4141 gtagccggga aggaaggccc atccacctga gcaacgtccg gcgaagggtc tggataccgg
     4201 cgctcaagag ggcagggctt gcgatcaggg aaatgaagca aacccggcac acgtttgcga
     4261 cagtggcgct gagctgcggt gagaaccccc tgtggatcgc aaaaaccatg ggacaccgca
     4321 actcggagat gatcatcaag gtctacagcc ggtacgtcga gaacatccgc ggaacggagg
     4381 atggctcgat catggaccgg atctatcagg taaacaaagg taaggaagag taagaggagg
     4441 taaaaaccat ttttggcaaa attatggcaa aacttgggtc caaaaagaaa aaggggctat
     4501 caccctggaa ggctaacccc ttgattttct ttggagccgg cgatgggatt cgaacccgcg
     4561 acctgctgat tacgaatccc ttgcaaacca gttttcccca tttttcgttt ttgcgagatt
     4621 gcttgacaca tcaatggctt agggctatct tgccttcgac gcgttgggtt gattttgggg
     4681 tttgttttcg gaaaatttac acactattta cacacccgac agccgaagcg gaaccgtaga
     4741 ggggggcaaa tgagcatcca gatattttgc acgagatgca aaaccggaag tgcacttggc
     4801 ggcggcgaac actgcccgaa gtgcggcgta ccctttggcc gggataggaa attccgggtt
     4861 caagtctccg tcaaagggga gcgagtaacc cgcgtgacgg acagcctcac cattgcccgc
     4921 gagttggagg caagcatcaa ggccgacctg gtgcgggaag agttcgacat cacccaccac
     4981 cggcagaaaa aaaaaccgat caccctgaat gacttgtggg acaagtacct gccatgggcc
     5041 aaggggaaca aacgaacatg gagaaatgac ctgtacctgt acgatgccca cattggacct
     5101 cgtttcggca gcaagcccct cgctgacatc acaccctttc tgcttgagcg gatgaagaac
     5161 gagatgaagc ggtccaggaa caagcaagga aagcccttcg ctgcggcaac catcaaacac
     5221 gttcttgtcc ttgtgcgccg tcttttcaac ctggccggga aatggaacct gtaccaggga
     5281 gcgaaccccg tcaagtccgt ggaaatgccc aaggtggaca accaggttac cgaattcttg
     5341 tccgatgagg agctatcccg gcttctgaac gtgctcgaca actggcccta tgacgattcg
     5401 gcggcgttcg tgaagttcgc cttgtttacc ggcatgaggc gcggagaact cttcaagctg
     5461 acctgggatg ccgtggactt cgagcgggac atgatcaccc tggcagctcc caaggggggc
     5521 aagaccgcaa ccctaccgat ttgccgggaa gccatggatg cgatccggga cctaccgcga
     5581 acctcccggt tcgtctttcc gggcaagggg ggcaagcagc gaaccgattt caagggacct
     5641 tggggacgga tccggaaggc ggcagggctc
//
LOCUS       DAOOVC010000211         5527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8333_length_5527_cov_7.327668, whole genome shotgun sequence.
ACCESSION   DAOOVC010000211 DAOOVC010000000
VERSION     DAOOVC010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8333_length_5527_cov_7.327668"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLM79_16885"
     CDS             complement(<1..180)
                     /locus_tag="PLM79_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOV88034.1"
                     /translation="METPLRVVVIGGVACGPKTACRLKRLLLDAEVTMIERGSLVSYG
                     ACGLPYYVEGVFSDLE"
     gene            complement(611..1003)
                     /locus_tag="PLM79_16890"
     CDS             complement(611..1003)
                     /locus_tag="PLM79_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88035.1"
                     /translation="MAKRVLVVDDELDMRLFMTTLLETNGFKPYAAEDGKQGLELARK
                     HKPALIIMDVMMPKESGIYLYRELKNDPDLKNIPIIMVSAVAKKTFFHSQRVLDEYEG
                     GTIPEPTEYIEKPPEADELLGAVRKILG"
     gene            complement(1057..1557)
                     /locus_tag="PLM79_16895"
     CDS             complement(1057..1557)
                     /locus_tag="PLM79_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOV88036.1"
                     /translation="MLEYKNILFCTDFSEDANIAFLHALNLAKKYNAKLHILHVPHSP
                     FRYHRVVVDEHISDEKSDEGQAFYDKDVEEKAMERLRATYDKRMGDFGNYLLVVKSGA
                     PDVEIIRYAKKNDVDLIVMGTLGKSEQDRIDHGSTVASVSKYAHCHVMAIRNPAKQFI
                     LPGKMF"
     gene            complement(1628..2803)
                     /locus_tag="PLM79_16900"
     CDS             complement(1628..2803)
                     /locus_tag="PLM79_16900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOV88037.1"
                     /translation="MAEKSETILLVDDEEGIRKVLGISLMDSGYHVLTAASGEEALGI
                     FKEARPEIVLTDIKMPGMDGIELLRRIKEMSPDTEVIMITGHGELDLAIKSLQYEAAR
                     FITKPINHDALDIALKRVRERISMRKELLEYTQEVEQLVEEKAQKLIEAERMAAIGQT
                     VATLAHAIKNIMGGLKGGIYVVEKGIELDNRKYLMQGWQMIKGNVNKIHNLAMDLLTL
                     TKEREPDYRLCDPNEPAREVYQLMLPRARECGISLELQLDSHLTPVYLDPDGIHCCIL
                     NLVTNAIEACMSVECSTKLGEVLIRTVKQNGWAVVYEVIDNACGMDQETRNKIFRTFF
                     STKGTKGTGLGLMITRKIVHEHGGIIQVESEKGCGTQFILRLPGREMSPRDGRVFPG"
     gene            complement(2803..5076)
                     /locus_tag="PLM79_16905"
     CDS             complement(2803..5076)
                     /locus_tag="PLM79_16905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV88038.1"
                     /translation="MFRGFLSIYRSLISRLILLSGLVLLVSIFTWAYFNIQYQKRNTL
                     EALIESVDRLGNTIRLGTHYAMMLNSREDINEIIKNTSRQEGIEKVRIFNKQGQIKYS
                     NLPSEVDQISNIKDDACYICHKTEPPVWEVPIDERIRLFQSPAGYRLLGIISPIYNEP
                     GCSTGCHVHPADKKVLGALDVVVSLESTDRAVSSYERRIIALAIVSFLALSTIIACFL
                     YLFVNRPIKKLIGETQLIGQGKFQYQSDTDGGDEIGELSRAIHQMGRRIVEKQEELNK
                     QRMEYQELFEKVPCYITVQDRNLRLLRYNLEFTRRFSPSPGDFCYEVYKGRSEPCEVC
                     PVLQTFEDGGTHSSEEAGITKDGEITHWMVRTSAIRDTRGEISAVMEVCLDITQMKRL
                     EHEIRRSEEKYRDIFNNMPNPVFVLDRKTLIILDCNESVTPTYGYRRDELIKKSFLDF
                     FEDGEQQQCALEMRNSDHVNQVRHLVKDGRTIYVNMHLSPSEYMGKEALLVVTSDITK
                     RLQAEQQLIQASKMATLGEMATGIAHELNQPLSVIKTASSFLSRKVRRGEPIKGEILQ
                     TMAEEIDGHVDRASMIINHLREFGRKSEVKRERVQVNEVLQKALDIFSQQLKLREIQV
                     VKEFQEDLPLILAVANPLEQVFINLLINARDAIEEKIEKLGSTQIPKRILLKTGSKNG
                     KVTVEVRDTGMGIPKPVLDKIFEPFFTTKKVGKGTGLGLSISYGIVQDYEGTIRVETR
                     ENEGSSFIVEFPVQSEG"
BASE COUNT         1046 a         1581 c         1550 g         1350 t
ORIGIN      
        1 ctcgaggtcg gaaaagactc cttccacgta gtaaggcaac ccgcaggctc cgtaggacac
       61 cagggaacct ctctcgatca tggtgacctc tgcgtccagg agcagccgtt taaggcggca
      121 tgccgttttg ggcccgcagg ccactcctcc gatgacgacc acgcgcaacg gtgtttccat
      181 ggatttcctc cttgcttcaa tgaatccttg ccggaagtcg aaccttcccc ggggttcccg
      241 gtggacttat cctccctatt ggtaagtgag tttactccag gagtgtcctt gaagtccatc
      301 ccggacactg cacccgatcc cgatcgaggc ggcgggcggc gggacggact ccgggcacgg
      361 atggggtccg gagagccgtg ggccctccgg cggacgggaa ctcccccggg tccctccacg
      421 cccctcgggc acccgcgagg gcgaaggcct tatgcccggg gcttttgaaa ggagagggtt
      481 cctggcctcg acccggggtt cgcaggggat tcaggaatac gggaacaaag aactcccccg
      541 caagcggggg aggagaggtc gggaatggtt gtttttcggc gggtggacgg cggagaatcc
      601 tccagtcgat tcagcccagg attttccgga cggcgcccag caattcgtcc gcttcgggtg
      661 gtttttcaat gtattccgtg ggttctggaa tggtgcctcc ctcgtactcg tccaggactc
      721 tctgggagtg gaagaaggtc tttttggcca ccgccgaaac catgataatg gggatgttct
      781 tgaggtccgg atcgtttttc agctctcgat ataggtaaat cccgctctcc ttgggcatca
      841 tcacatccat gatgatgagg gccggcttgt gttttcgggc aagctccagt ccctgctttc
      901 catcctcggc cgcgtaaggt ttgaagccgt tggtttccaa aagagtggtc atgaacagcc
      961 gcatgtccag ttcatcgtct accaccaata ctcttttcgc catgggtctt ccctccagaa
     1021 ttcttcactc gatccgcgtt acaaaaggca gggattctag aacatcttgc ccggcaggat
     1081 gaattgcttg gccggatttc gaatggccat cacgtgacag tgggcatatt tggaaacgct
     1141 ggccacggtg ctcccgtgat cgatcctatc ttgctccgat tttcccaagg tgcccatgac
     1201 gatgaggtcc acgtcgtttt tcttggcata tcgaatgatc tcaacgtcgg gggctccgga
     1261 tttcacgacc aagaggtagt tgccgaaatc gcccatgcgc ttgtcgtagg tcgccctgag
     1321 gcgctccatg gctttctctt ccacgtcctt gtcgtaaaag gcctgtccct cgtcggactt
     1381 ctcatccgag atatgctcgt ccacgacgac ccggtggtaa cggaacgggg aatgaggcac
     1441 gtgaagaatg tgcaatttgg cgttgtattt tttcgccaaa ttgagggcgt gcagaaaggc
     1501 gatgttggca tcttcggaaa aatccgtgca aaaaaggatg ttcttgtatt ccagcatgat
     1561 ggttccttct ccttaggctt caacgatcca agtttctctc ttcatcccaa aacggttcgg
     1621 tcaaacgtta ccccggaaat acccggccgt ctcgggggct catctccctc ccgggaagcc
     1681 gaaggatgaa ctgggtcccg caccccttct ccgattccac ctggataatg cctccgtgtt
     1741 cgtgcacgat ttttctggtg atcatgagtc ccagcccggt ccccttggtg cctttggtac
     1801 tgaaaaaggt ccggaaaatc ttgttcctgg tttcctgatc catgccgcag gcgttgtcga
     1861 tcacttcata gacaacggcc catccgttct gcttgacggt gcgaatgagt acttctccca
     1921 gttttgtcga gcattccact gacatacatg cttcgatggc gtttgtcact aggtttagga
     1981 tgcagcagtg aattccgtcg ggatcgagat acaccggggt tagatgggaa tcgagctgca
     2041 attccagtga aatcccgcac tcgcgagccc ggggaagcat gagctggtat acttcccggg
     2101 cgggttcgtt ggggtcgcac aaacggtagt cgggctcccg ctccttggtg agggtcaaca
     2161 gatccatggc cagattgtgg atcttgttga cgttgccctt gatcatctgc caaccctgca
     2221 tcaggtactt tctattgtcg agttcgatcc ccttttccac cacgtagatg ccgcccttga
     2281 gaccgcccat gatgttcttg atggcgtggg ccaaggtcgc caccgtctgg ccgatggcgg
     2341 ccattctttc ggcttcgatg agcttttgcg ccttttcctc caccagttgc tcgacctcct
     2401 gggtgtattc caggagctct tttcgcatgg agattctctc ccgcacacgc ttgagggcga
     2461 tatccagggc atcgtgattg atgggcttgg tgatgaacct ggccgcttcg tattgaagac
     2521 tcttgatggc caggtcgagc tctccgtggc ccgtaatcat gatgacctcc gtgtccggac
     2581 tcatctcctt gatgcgacgg agaagctcga ttccgtccat ccccggcatc ttgatgtccg
     2641 tgagaacgat ttccgggcgg gcctccttga aaatccccag ggcttcctcg ccgcttgcag
     2701 cggtcaacac gtggtagccg ctgtccatga gcgagatccc caagaccttt cgaatgccct
     2761 cttcatcatc caccaacagt attgtctcac tcttttcggc catcagccct cactctgaac
     2821 aggaaactcc acgataaagc tcgaaccctc gttttcccgg gtctccactc ggatggtccc
     2881 ctcgtagtcc tggacgatgc cgtaactgat ggacaaaccg aggcccgtgc ccttgcccac
     2941 tttcttggtg gtaaagaacg gttcgaagat cttgtcaagt accggtttcg ggattcccat
     3001 ccccgtatcc cggacttcta cggtcacttt gccgttcttg gagccggtct tcaggaggat
     3061 tctcttgggg atctgggtgg aaccgagctt ttcgattttc tcttcgatgg cgtctcgggc
     3121 gttgatcagc aggttgatga acacttgctc caacgggttc gcgacggcca gaatgagggg
     3181 caggtcctcc tggaactcct tgacgacctg gatctctctt agcttcagct gctggctgaa
     3241 gatatccagg gccttctgaa gcacctcatt gacctggaca cgttccctct tgacctcgga
     3301 tttccggccg aattcccgta ggtgattgat gatcatggaa gcccggtcca catggccgtc
     3361 gatctcctcc gccatggttt gcaggatctc ccccttgatg ggctctcccc tgcggacctt
     3421 ccggctcaaa aagctgctgg cggtcttgat gaccgacaaa ggctgattca gctcgtgggc
     3481 gatccctgtg gccatttccc ccagggtggc catcttgctc gcctggatga gctgctgctc
     3541 ggcctgaagc ctcttggtga tgtcgctggt cacgaccagc aaggcttctt ttcccatgta
     3601 ttccgaggga gacaggtgca tgttcacata aatggtcctg ccgtccttga ccaagtgcct
     3661 cacctggttc acatggtccg aattgcgcat ctccagggca cactgttgtt gttccccgtc
     3721 ctcgaaaaag tccaggaatg atttcttgat cagttcgtcc cgccggtacc cgtaagtagg
     3781 cgttacactc tcattacagt ccagaatgat caaggttttt ctgtccagga caaagacggg
     3841 attgggcatg ttgttgaaga tgtcccggta cttctcctcg gatcgtcgga tctcgtgctc
     3901 gagccgtttc atctgggtga tgtccaggca cacctccatc accgcggaga tctcccctcg
     3961 ggtatcccgg atggcggagg ttcgcaccat ccagtgggtg atctccccgt ccttggtaat
     4021 cccggcttct tcactggaat gggttccccc gtcctcgaag gtttggagga ccgggcatac
     4081 ttcgcacggc tccgaacgtc ccttgtacac ctcgtagcag aaatcgccgg gactggggga
     4141 aaaccgtctt gtgaattcga ggttgtagcg aaggagcctc aggttccggt cctgcaccgt
     4201 gatgtagcag ggaacctttt cgaacagttc ctggtattcc atccgctgct tgttgagctc
     4261 ttcctgcttc tcgacgatcc ttcttcccat ctgatggatg gcacgggaca gctcgccgat
     4321 ttcgtcccct ccgtcggtat ccgactggta ttggaacttt ccctggccga tgagttgggt
     4381 ttcaccgatg agcttcttga tggggcgatt gacgaacagg tagaggaaac aggcgatgat
     4441 ggtggatagg gccaggaagc tcacgatggc cagggcgatg atgcgccgct cataggacga
     4501 aacggcccga tccgtgctct cgagggacac caccacgtcc agggccccca ggaccttttt
     4561 gtccgccgga tgaacatggc agcccgtgga acagccgggc tcgttgtaga tggggctgat
     4621 gatccccaga agccggtacc ccgcagggga ctgaaagagg cgaatccgtt cgtcgatggg
     4681 aacttcccag acgggaggtt ccgtcttgtg gcagatatag cacgcatcgt ctttgatatt
     4741 ggaaatctgg tccacttcgc tgggaagatt ggagtacttg atctgacctt gtttgttgaa
     4801 gatccggacc ttctcgatcc cttcctggcg actggtgttt ttgatgatct cgttgatgtc
     4861 ttcccgggaa ttcagcatca tggcgtaatg ggttcccagg cggatggtgt tccccaatcg
     4921 gtctacggac tcgatgaggg cttcgagggt attccttttc tggtactgga tgttgaaata
     4981 ggcccacgtg aaaatactga cgagcaaaac gagacccgac aggagaatga gccgggaaat
     5041 caagctccga taaatgctga gaaaccctcg aaacattgct gatgatccga tcctttcgca
     5101 aagcccacca agcgggaccg tgtccggggg gtggtgtatt tgcccggttc ttgcgctcaa
     5161 cgagtcgtga ccagggcagc catggaagca acttgctggg ggaacggcgg cttgcgcgct
     5221 cagtcggctg cggagaagga aggattggac cctagaaacg gacgatccgg tcaggcgcca
     5281 ccccgtgcca ggggaatctg ccggccccgg gggggccggg atagtcccaa atcccgtctg
     5341 gacgggtctt ttccataaaa actctagcct aaaacaagcc atgggtcaag aatgaagccc
     5401 caccggaggg agtgcgggcg cgggcttgcc gggggccgcc catcccctgc ccggccctgg
     5461 tttcatggga ccctcgtcct gagtcccttc cccctccgat ccccccgaac aagggggtcc
     5521 ggatttg
//
LOCUS       DAOOVC010000212         5521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8346_length_5521_cov_3.933041, whole genome shotgun sequence.
ACCESSION   DAOOVC010000212 DAOOVC010000000
VERSION     DAOOVC010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_5521_cov_3.933041"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLM79_16910"
     CDS             complement(<1..956)
                     /locus_tag="PLM79_16910"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HOV88039.1"
                     /translation="MLYDHIVAIQRKKPLFNRRPLSIEDLVEWCEEIGAKLIQDHRAR
                     HASAYWDGGRPIIRLNPNSSEALQIIILGHEIGHILLGHLEIGSELPFSIHNLFLKNR
                     WERDASVIGYLCLIPTAELIRMVRNDIQGPEELFSELQPMFGDDEKLGMEICVERMKI
                     FIALLDNCGGKCLEGEDCHVCGEAPPRPGPAAAFFNRPPADVRVVGSPYDVEPGGEYR
                     FRSDGANVVRIRFQPPVTGRTIRLFARNVPVAGVSFGEALGNTTAFISGRDRSLILER
                     DPGNQFDPNAIRVIARWRGATGSEHQAQIGWVPRAVAARVAHR"
     gene            complement(988..1494)
                     /locus_tag="PLM79_16915"
     CDS             complement(988..1494)
                     /locus_tag="PLM79_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88040.1"
                     /translation="MTTKAYDRFLAAFRALVDSEGWGVQARLARSVGKTARHINGIYK
                     GKMGASLDLQENIAAFFGMSYEDILARGREILASQSRPARRDEKPVPFQDKLDAVGRN
                     NKLAARLIADEAARAVGYPNFMAAIIDSVGIYPPGWKEYLADEISAYDFWEETKQHVL
                     NQVGKLKE"
     gene            1616..1843
                     /locus_tag="PLM79_16920"
     CDS             1616..1843
                     /locus_tag="PLM79_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88041.1"
                     /translation="MIDGLKHGEQAGLARKIGITPRHMNDIIKQRKRPSRTLSEKLEA
                     ETGISAEAWLFPDKHPNPLLKGNTPLKAPPP"
     gene            2194..3459
                     /locus_tag="PLM79_16925"
     CDS             2194..3459
                     /locus_tag="PLM79_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88042.1"
                     /translation="MRDYGKIYVSFWTSADTSSLSMEAKLLGAYLLSSSHTNMIGCFR
                     LPVQYVCSDLQFASESVLNAFAELSTIGFATYDEKHQWIIIHNFLKWNRIENHNQGKA
                     AMKLAEQIPSRSILNPYMVKSLETFGKHLPESAMKQLRKRIDGGEKPCETHREPFPEG
                     SETVSKGFPKGFETVSEPAGNPEGQESPGAMSDASPPAEMFSASTAAESAGGPESPST
                     ADLETVSKGFPKGFETLPETVRKPFRNQEQEQEQEQEKNKRYSASPAPGEPESAASPP
                     GPSAPDGAVLDQRYLTRRRRKLSGWKLEAFEEFWSTFAYKKGRAEAADAWLDIPDLTR
                     ELAARITKAAAREARARPGLIARGGTPKMAQGWLSARRWEDWENNDQPPDGPGGAHGP
                     KAGDDADPSGIDEEAQYRESIRDAVKRFM"
     gene            3479..4216
                     /locus_tag="PLM79_16930"
     CDS             3479..4216
                     /locus_tag="PLM79_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88043.1"
                     /translation="MVIRIELFGETITLRPACECRLKDLEEREKAARIANLCGLIRAQ
                     GLDDGLYAQMSLESWKRHDSSSEEAAKKLEAYLRNVGHGARNWLFLYGDYGLGKTHLA
                     VSALRYLCLNRQWEPLFIKWSEYCGRIQQSWNDPAADSEYDLWRKPANVPLLVLDDID
                     KRASSEWALGKLYDLIDHRYVRRLPTILTANRGIEALAGFWGRSPQMKDLSRAITSRI
                     VGQLSAAIEFSGKDYRLGKAEVEKVRS"
     gene            4403..4900
                     /locus_tag="PLM79_16935"
     CDS             4403..4900
                     /locus_tag="PLM79_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88044.1"
                     /translation="MIICACDPDCRTPAFALFDGTRLMKWKLLDTGRTGHIDRVLPDI
                     QAIIDAWRPGLLVIENQYLPTGPEAAKRFKPVSQLVAARGMITAIFAISNIEYRLIEP
                     FAWQKTLGGAGLGRDQLKRRSILKATDIAGALIEDHNVADAINIGYFYATRNRLTRKA
                     SECVR"
     gene            5050..5289
                     /locus_tag="PLM79_16940"
     CDS             5050..5289
                     /locus_tag="PLM79_16940"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOV88045.1"
                     /translation="MSPTNMLSEMAMKEYYLVREVAEHFRLSPRTIYRLIERGELAAT
                     RIGGSQRIPASEISNFKPRRRRRKKMKPCPKKSVN"
BASE COUNT         1318 a         1626 c         1504 g         1073 t
ORIGIN      
        1 cggtgtgcaa ctcttgcggc aactgctctg ggcacccagc cgatttgggc ctggtgctcg
       61 ctccctgttg ctccccgcca ccgggcgatt acccggatgg cgttggggtc gaactggttg
      121 ccggggtccc gctcgaggat gagcgatctg tcgcggccgg aaatgaaggc ggtggtattt
      181 cccagcgcct cgccgaaaga aactccggca acgggcacat tgcgggcgaa gaggcgaatg
      241 gtccttccgg tcactggcgg ctggaacctg atcctgacga catttgcgcc atcgcttcgg
      301 aaccggtatt cgccaccggg ctccacatcg taaggagagc cgaccacacg aacatcagcg
      361 gggggccggt tgaaaaaagc ggctgccgga cccggcctgg gcggagcctc tccgcagacg
      421 tggcaatcct ctccttccag acacttgcct ccgcagttgt ccagaagcgc gatgaagatc
      481 ttcatgcgct cgacgcaaat ctccattccg agcttttcat cgtccccgaa catgggctgc
      541 agctcggaaa acagctcttc cggcccctgt atgtcgtttc gaaccatcct gatgagctcc
      601 gctgtcggga tgaggcaaag atagccgatg acggatgcgt cgcgctccca ccggtttttc
      661 aggaagagat tgtggatgga gaagggaagc tcggagccta tttcaagatg cccgaggaga
      721 atgtggccta tctcgtggcc gagaatgatg atttgaaggg cctccgagct gttcgggttc
      781 aaccggatta tcggacgacc accgtcccag taggccgagg cgtgcctggc tcgatggtcc
      841 tggatcagct tcgccccgat ctcctcacac cactcaacca ggtcctcaat ggacagcggt
      901 cttcgattga aaagcggctt cttcctctga atggcgacaa tgtggtcata gagcatgcaa
      961 gcccctccgc ttggtgctct cgaacgatta ctccttcagt tttcccacct ggttcaaaac
     1021 gtgctgtttg gtttcttccc agaagtcgta cgccgatatc tcgtcggcga gatactcttt
     1081 ccagcccgga ggatagatcc cgaccgagtc tatgatggcg gccatgaaat tgggataacc
     1141 cacggccctt gccgcttcgt cggcgatcag gcgggcggcg agcttgttgt tgcggcccac
     1201 cgcgtcgagc ttgtcttgaa acgggaccgg cttttcatcc cttcgagccg gcctggattg
     1261 agaggccagg atctcacgac ctctggccag gatatcttcg tagctcatcc cgaagaaagc
     1321 ggcgatgttt tcctgcaaat caaggctggc tcccattttt cccttgtaga ttccgttgat
     1381 gtgccgggcc gtttttccca ccgatctcgc caggcgagcc tgtacccccc acccttccga
     1441 atccacaagg gcgcgaaatg ccgccaggaa tctgtcgtat gcctttgtcg tcatgaaagc
     1501 attatagaga ttgcggttct attttgcaat aggaaaaata attctatttt tcccttgacg
     1561 atggaaacag tatttctata attagcctcc aaattataga agaggaggtt cgagaatgat
     1621 cgatggattg aagcatggag aacaggccgg cctggccagg aagatcggta ttaccccccg
     1681 ccacatgaac gacatcatca agcaaagaaa acgaccgtcc agaacactca gcgaaaagct
     1741 cgaggccgaa accggcatca gcgccgaggc ctggctcttc ccggacaagc acccaaatcc
     1801 cctgctcaaa ggcaacacac ctctcaaggc cccaccgcca tgatccacag attacacaga
     1861 ttttcacaga tttaagagaa ggttcttcat ctgtgtaaat ctgtgaaatc tgtggataaa
     1921 gatgaagggt acgaagttgc agacgttttt aatttgtacc gaccagacat gccgccacac
     1981 agcaagatca ttcccttggg acagcgaaat gtaaccgagt tgggcggagc ctgcgcgccg
     2041 cgaggcggcg ggttttaggt tttaagtttt aggttttaag tatggggctc gaagtctcac
     2101 accactcctg aatacccact caagtcattc acttaaaact taaaacctaa aacttgaaac
     2161 ccaaaaccca gccccaaact ttcgaagaag accatgcgag attacggaaa gatctacgtc
     2221 tcgttctgga cgtccgcgga tacgtcatcg ctgtcgatgg aagcgaagct ccttggagcc
     2281 tatcttcttt caagctcgca cacgaacatg atcgggtgtt tccggctgcc cgttcaatac
     2341 gtgtgttcgg acctgcaatt cgcctccgaa tcggtcctga acgcatttgc cgagctttcg
     2401 accatcggtt tcgccaccta cgacgaaaag caccagtgga tcatcatcca caacttcctc
     2461 aaatggaacc gcatcgaaaa ccacaaccag ggcaaggcgg ccatgaagct cgccgagcag
     2521 atcccatccc gatccatcct gaatccgtac atggtgaagt ccctcgagac ttttggaaag
     2581 cacctgcccg agtctgccat gaagcagctt cgaaagcgca tcgatggggg cgagaaacca
     2641 tgcgaaaccc accgggaacc gttccccgag ggttccgaaa ccgtttcgaa agggtttccg
     2701 aagggtttcg aaaccgtctc ggaacccgct ggaaacccgg aggggcagga gtcgcccggc
     2761 gccatgagcg atgcaagccc gccggcagaa atgttttccg catcaacggc ggcggaatcg
     2821 gctggaggac ctgaaagccc gtccacagcg gatcttgaaa ccgtttcgaa agggtttcca
     2881 aagggtttcg aaacgcttcc cgaaaccgtt cggaaaccct ttcgaaacca ggaacaggaa
     2941 caggagcagg aacaggaaaa gaataaaaga tattctgcat cgccggcgcc gggtgagccc
     3001 gaaagtgcgg catcgcctcc cgggccttcc gcacctgacg gtgcggtact cgatcaacgc
     3061 tatttgacca ggcgtcggcg gaagctctcc ggctggaagc tcgaggcttt cgaggagttc
     3121 tggagcacat ttgcatacaa gaaaggcagg gccgaagcgg ccgacgcctg gctggatatt
     3181 cccgacctta cccgggagct cgccgctcgc atcacgaaag ccgcggccag ggaggccagg
     3241 gcgcgcccag ggctcatcgc caggggagga acgccgaaaa tggcccaggg ctggctctcc
     3301 gcccgcaggt gggaagactg ggagaacaac gaccaacccc cagacggccc tggcggtgct
     3361 cacgggccga aggccggcga tgatgcggac ccatccggaa tcgatgagga ggcccaatat
     3421 cgtgaatcca tccgagacgc agtcaaacga tttatgtgaa cactgcgggc agccgctcat
     3481 ggtgataagg atcgagctct tcggcgaaac gatcaccctc cggcccgcct gcgagtgtcg
     3541 gctcaaagac ctcgaggagc gtgagaaggc ggcccgcatc gcgaacctgt gcggactgat
     3601 ccgtgcccag ggcctcgatg acgggctata cgcgcaaatg agcctcgaga gctggaagcg
     3661 tcacgactca tccagcgagg aagcggcaaa gaagcttgaa gcgtacctcc ggaacgtcgg
     3721 ccacggagcc cggaactggc tcttccttta cggggactac ggcctcggta agacgcacct
     3781 tgcggtttcc gcgctccgat acctgtgcct gaaccgccag tgggagcccc tgttcatcaa
     3841 gtggtcggaa tactgcggcc gcatccagca aagctggaac gatccggcag ccgacagcga
     3901 gtacgatctc tggcgcaaac ccgccaatgt cccgctcctc gtcctggacg acatagacaa
     3961 gcgagcctcc tccgagtggg cgctcggcaa gctctatgac ctcatcgatc accgctacgt
     4021 ccgccggctc cccactatct tgacggcaaa ccgcggcata gaagccctcg ccggcttctg
     4081 gggcaggagc ccgcagatga aagacctcag ccgagccatc accagccgca tcgtcgggca
     4141 gttgtcggct gccatcgaat tctcggggaa agactaccgg ctgggtaaag cagaagttga
     4201 gaaggtaaga agttgagaag ataagaacat cttttcgacc aatacaatcc acagattaca
     4261 cagattttca cagattgaat cattattgaa tccgttatct gtgtgaatcc gtgaaatctg
     4321 tggatgatca aagcgtcttc tcgacctccc atcttcttat cttctcatct tctcaacttc
     4381 tgcccttcaa aggagctact tcatgattat ctgcgcgtgt gatcccgact gcaggacccc
     4441 tgcattcgct ctcttcgacg gtaccaggct gatgaaatgg aagcttctcg ataccggcag
     4501 aacgggacat atcgacaggg tcttgccgga catccaggcg atcatcgacg cctggcggcc
     4561 gggactcctc gtcatcgaaa accagtatct tccgacggga cccgaagccg ccaagcgctt
     4621 caagccggtc tcccagctcg tcgcggcccg cggcatgatc accgccatct ttgccatatc
     4681 caacatcgaa taccggctca tcgaaccgtt cgcatggcaa aagaccctcg gcggcgccgg
     4741 ccttggaaga gaccagctca agcgccgctc gatcctgaag gccacggata ttgccggcgc
     4801 actgatcgag gaccacaacg tcgcggacgc catcaatatc ggctacttct atgccacaag
     4861 aaaccgcctc acgcgcaagg caagcgaatg tgtcaggtag ccgcagccgg ccggggtcag
     4921 gtctacacat ttgacgagtc aacaaacaat ggaaccgcaa agcctgtccc cgcaggtctc
     4981 aggcggggaa cgcaaagggc gccaagaacc gagttctctt cgcgtccttc gcgccttagc
     5041 ggttcaaata tgagtccaac caacatgctg agcgagatgg caatgaagga atactacctg
     5101 gttcgagaag ttgcagagca tttccgcctc agcccccgga cgatctaccg cctgatcgag
     5161 aggggtgagc tcgccgcaac caggataggc ggctcccagc gcattccggc cagtgagatc
     5221 tcaaacttca aacccaggcg aaggcgcagg aagaaaatga agccatgccc aaaaaagagt
     5281 gtcaactgat gacatttgcg acagctccga tttgcaatcg agtggagcaa gcagttatca
     5341 gggcagcgga acgaggacca attagcggaa aaagcagtca aggcaaccca ttccaattca
     5401 ttcatctctc aaaaaggagg gaaagagcca tggcaacgag aatcgatgta gcaagggcag
     5461 tagcggagaa gcaaaagatc tccatccagg acgccaactc catcatcgga accaccatcg
     5521 a
//
LOCUS       DAOOVC010000213         5486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8399_length_5486_cov_5.708893, whole genome shotgun sequence.
ACCESSION   DAOOVC010000213 DAOOVC010000000
VERSION     DAOOVC010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8399_length_5486_cov_5.708893"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLM79_16945"
     CDS             complement(<1..407)
                     /locus_tag="PLM79_16945"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOV88046.1"
                     /translation="MMNRQRKPKDRLLQEILHLCEQLAELRARELASHPSRDIFFADQ
                     QRCMDVIEFLPDATFIVDGNGKVIAWNRAMEELTGVSKKDVLGKGKFEYSIPFYGKAR
                     PILIDYALAKEPGVDHYYDFVQKDANTIMAEVFA"
     gene            1134..2384
                     /locus_tag="PLM79_16950"
     CDS             1134..2384
                     /locus_tag="PLM79_16950"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOV88047.1"
                     /translation="MEATVNPFKIAQQQLDEVAGILGLDPATHELLRRPMRETRASLP
                     VKMDDGSTRIFEAFRVQYNTARGPAKGGIRWHERETLDTVRALAAWMTWKTAVVDIPL
                     GGGKGGVVCDPKELSEAEKERLARAYVRAFASFLGVTKDVPAPDLYTTPQIMAWMMDE
                     YETILGENHPGVITGKPISLGGSKGRSDATARGGIYVAREAAKLYGINLEEEPMAVMG
                     FGNVGHHAAILGEEILGMKLVAASDSKGGVYNPSGMSPRALLDHKLRNGDFSGFPEGD
                     AITNEELLELPLTVLFPAALENVITEENAPKLRCRICCELANGPTTPEADRILADRDI
                     LVLPDFLANAGGVTVSYFEQVQNAYNLYWEIQEVHWRLDKKMTRAFAGVHDMSQRNKV
                     SLRLAAYLVAVSRVAEACKLRGWV"
     gene            2505..2684
                     /locus_tag="PLM79_16955"
     CDS             2505..2684
                     /locus_tag="PLM79_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88048.1"
                     /translation="MFESAPPGRTPGAAPPALPNPAAFALAVYPTIRFYDGRSANRFR
                     LQELPDPITQVTWSG"
     gene            2973..3494
                     /locus_tag="PLM79_16960"
     CDS             2973..3494
                     /locus_tag="PLM79_16960"
                     /inference="COORDINATES: protein motif:HMM:NF023249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HOV88049.1"
                     /translation="MIAHTDGSHTQHDRKMVEVMTIRDLHKAFEFCILLGVLTNLAGM
                     LLLARLPQPRLPAYYDPRFTEDRFAVVVPYVVGEKGRILSILQRSGMTEIREVEGLRS
                     HESPGVQPGHAPEFFSLLLQLPGNSLGRLGSLEPGDCGPGHVCGQPDRHRQHLRFSGA
                     FSPKEPKGLPKEP"
     gene            complement(3460..5367)
                     /locus_tag="PLM79_16965"
     CDS             complement(3460..5367)
                     /locus_tag="PLM79_16965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HOV88050.1"
                     /translation="MIHGEKGHAPSDSTGPPPAPPFLGIDPRGKTPEDLEREWVENHY
                     RGDIPQLTPRAVFIGGILGGVMSLSNLYIGLKTGWGVGVAITACILSFAVGTVLSKIG
                     LFRSNLSILENNCMQSTASSAGYSTGSMLVSAVPALLMIEGRSLPFGILLLWTIFLAL
                     LGVILAIPMKRHMINIEQLKFPSGIAAAVTLRSLYGAGADALKRGRRLAVSAVLGSGL
                     AFARENLLGWWPAALRLPGMIPIPGTLAGHRLVEWTISFELSGIMIAAGAIVGIKVAC
                     SMMAGALINYGILAPMMHEQGVIRELGYRGIVSWSLWGGTALMVASGLFHFAAQWRTL
                     GRAVGTLRAAFSPSRKEGKGVDDRPAGIEVPGSWFLTGLLVAGVGIVWIQITAFAISW
                     WMALLSVLLTFFLTVVACRAAGETDITPIGAMGKITQLTFGVIAPTNTTANLMTACVT
                     AGAAASSADLLTDLKSGYLLGANPRKQFIAQFLGIFAGAAVIVPAFLLLVPDAAVLGG
                     DRFPAPGAQVWKGVAELLARGLSSLHPTAQWALLVGAVLGVLLAAVEQAASPRLRRFL
                     PSAMGLGISCVIPFWNTLSMFLGALAARLMALRDPDVTDKTVIPVASGLIAGESLMGV
                     LMALWAALWAL"
BASE COUNT         1069 a         1743 c         1692 g          982 t
ORIGIN      
        1 gcaaacacct cggccatgat ggtgttcgcg tccttctgga cgaaatcgta atagtggtcg
       61 actcccggtt ccttggccag ggcgtaatcg atgagaatgg gccttgcctt tccgtagaac
      121 gggatcgagt attcgaactt gccttttccc agcacatcct ttttggacac tcccgtgagc
      181 tcctccatgg cacgattcca ggcaatgacc ttgccgtttc cgtccacgat gaaggtggcg
      241 tcggggagga attcgatgac gtccatgcac ctttgctggt ccgcgaaaaa gatgtctcgg
      301 gaaggatggg aagcgagctc ccgggccctg agctcggcca attgctcgca taagtgcagt
      361 atctcttgga ggaggcgatc cttgggcttc cgctgtctgt tcatcatgcg agtctccgct
      421 gcgaagtcat aggccaacac gaacatgagg atccgaagta ggaagaattg tctccattgt
      481 agcagtactc ctttgaatgt caaagggaag ggcgagaggc cgggaggctc ggaggcggtg
      541 tagggtgggt ggagcgaagc aaagcccacc ggggggcggg gagcgagagg ctagttagtt
      601 ggttggttgg ttggttgggg gcgaggggat cgaggcgagg ggcgaggggc gagaaaccgg
      661 gggcattggt agctttggtg gcgttggttg gggggcgcag ggccgggggt caaaccggag
      721 gcattgggtt tccttggttt ttgggcaaag ggggtttgct tcccatcttc ccatcctccc
      781 ggcttcccag cctccagcct ccagcccctt gccctctgcc aaccaatcta ccaacgctct
      841 ggccgtatcc ttccggttgt ggtaaaggtg gcacgggaag gctgggcaaa tgcgttcttt
      901 ctcccaggga gaccgggctc acctacggcg acgagggttc ttttccgcac ggttgcccgc
      961 ttcggccgcc cctttcccgg ggagccgggg gaaacgacaa aacccgcgcc ccatagaacc
     1021 gaccgaacca aggaaccaat ggatcaacga accatgaaac cggctgaacc aatcgaaccg
     1081 acgaactaac gaaccaactg cccaatccaa cccaaccacc cgaggactcg cccatggaag
     1141 ccaccgtcaa tcccttcaag atcgcccagc aacaactgga tgaggtcgcc ggaattttgg
     1201 gattggaccc ggcgacccac gaactgctgc gccggcccat gcgggagacc agggcctctc
     1261 tccctgtaaa gatggacgac ggatccaccc gcattttcga ggcgttccga gtccagtaca
     1321 acacggcccg gggacccgcc aagggaggga tccggtggca cgagcgggaa accctcgata
     1381 cggtgagggc cctggccgcc tggatgacct ggaagaccgc cgtggtggac atccccctcg
     1441 ggggcggaaa gggaggggtg gtgtgcgatc ccaaggaact ctccgaggcc gaaaaggaac
     1501 gcctggcccg ggcttatgtg cgtgccttcg cttccttcct gggagtcacc aaggacgttc
     1561 cggcccccga cctgtacacc actccccaga tcatggcctg gatgatggat gaatacgaaa
     1621 ccattctggg cgagaatcat cccggggtca tcacgggaaa acccatctcc ctgggaggct
     1681 ccaaaggccg aagcgatgcc acggcacggg gaggaattta tgtcgcccgg gaggccgcca
     1741 agctttacgg gatcaacctc gaggaggagc ccatggcggt aatgggcttt ggaaacgtcg
     1801 gccatcatgc ggcgatcctg ggagaagaaa tcctgggcat gaagctcgtc gccgcatcgg
     1861 actccaaagg cggcgtctac aatccctcgg gaatgagccc ccgggccctc ctggatcaca
     1921 agcttcgcaa cggcgatttt tccgggttcc ccgaaggcga cgccatcacc aacgaagaac
     1981 tcctcgaact gcccctgacg gtcctttttc ccgccgctct cgagaacgtc atcaccgaag
     2041 aaaacgcacc caagctccgg tgcaggatct gttgcgaact ggccaacggc cccaccaccc
     2101 ccgaggcgga ccgaattctt gccgaccgag acatcctggt cctgccggat ttcctggcca
     2161 acgccggggg agtcaccgta tcctatttcg aacaggttca gaacgcctac aacctctact
     2221 gggagatcca ggaagtccac tggcgattgg acaagaaaat gacccgcgcc ttcgcggggg
     2281 ttcatgatat gagccaacga aacaaggtga gcctccggct ggctgcctat ctggtggccg
     2341 tttcccgggt ggccgaggcg tgcaagcttc gaggctgggt ctagagagcc gccctcctgc
     2401 ttatcgaggc ccccccggcc gagctttccc ctcgttttcc ccgctccctg cccccgggta
     2461 ccgccttcgg gcctcgggat cctgccgttg ttcgttcccg ggctttgttc gagtccgccc
     2521 ctcccgggcg cactcccggt gccgcccccc ctgccctgcc gaaccccgcc gccttcgccc
     2581 tggcggtcta tccgaccatc cggttttatg acggcaggag cgccaaccgg ttccggctcc
     2641 aggaactgcc ggaccccatc acccaggtca cctggagcgg ctaggtcgag attcaccacg
     2701 aaacggcacg aaaacgggac atcgccgggg gcgacctgtt gaaggttccc tctcttttcg
     2761 gcagcatcct tgttccggcc ccgcccattc ccacagtatc tccggacacc ttggccattc
     2821 ccctggggca ggggcaccgt tcgttcggtc gcttccccga cgggttgccc gccaatccca
     2881 tggaactgtt cccatgggag atcgagcctc gaagcagggc catatccggc caagatctcc
     2941 gggtcctcct gaaaaagacc ggataggttt tcttgatcgc tcacacggac gggagccaca
     3001 cccagcacga ccggaaaatg gtcgaggtca tgacgatccg ggacttgcat aaagccttcg
     3061 agttctgcat tcttctcggg gtcctgacca acctggccgg gatgctcctc ctcgcccggc
     3121 tcccccaacc aaggcttccc gcctattacg atccccggtt taccgaggac cgatttgccg
     3181 tggtggtgcc ctatgtcgtc ggggaaaagg ggcgaatcct ctccattctt cagcgttccg
     3241 gaatgacgga aatccgggaa gtggagggtc tgagatccca tgaaagcccg ggcgtccaac
     3301 cgggtcatgc gccggaattc ttttccttgc ttctacaact tcctggcaat tcccttggcc
     3361 gtctcgggag ccttgaaccc ggggattgcg gccctggcca tgtctgcggg cagcctgacc
     3421 gtcaccgcca acaccttcgc ttttcggggg ccttttcgcc taaagagccc aaagggctgc
     3481 ccaaagagcc atgagcaccc ccatgaggct ttctccggca atgagacccg aggcaacggg
     3541 aatcaccgtc ttgtccgtga cgtcggggtc tcgaagggcc atgagccggg cggccagggc
     3601 ccccagaaac atggacaggg tattccagaa gggaatgacg caggatatcc ccaaccccat
     3661 ggccgacggc aggaagcggc gaagccgagg ggatgccgcc tgctcgacgg ccgccaggag
     3721 caccccgagg accgctccca cgagcaacgc ccattgggcc gtggggtgca ggctcgagag
     3781 gccccgggcc agcaattccg ccacaccctt ccacacctgg gcccccgggg ccggaaagcg
     3841 atcccctccc aggacggcgg cgtcgggcac cagcaggagg aaggcgggca caatgaccgc
     3901 ggcgcccgca aatatcccca ggaactgggc gatgaattgt ttccgggggt tggcccccaa
     3961 gagatagccc gacttgaggt ccgtgagaag atccgcggaa gaagcggccg cccccgccgt
     4021 cacgcaggcc gtcatgaggt tggcggtggt gttcgtgggg gcgatcaccc cgaaggtgag
     4081 ctgggtgatc ttccccatgg ccccgatggg ggtgatgtcc gtttccccgg cggctcggca
     4141 ggcgaccacg gtaagaaaga aggtgagcag caccgaaaga agcgccatcc accaggagat
     4201 ggcgaaggcg gtgatctgga tccaaacgat tccgacccct gccaccagga ggcccgtgag
     4261 aaaccagctt ccggggacct cgatcccggc cggtcggtcg tcgacgccct tgccctcctt
     4321 tcgggacggg gagaaggcgg cccgaagggt tcccacggcc cttcccaggg tgcgccattg
     4381 ggcggcaaag tggaacaggc ccgaggccac catgagggca gtccctcccc agaggctcca
     4441 ggacacgatc ccccggtatc ccagctcccg gattactccc tgttcgtgca tcatgggagc
     4501 caggatgccg tagttgatca gcgcccccgc catcatgctg caggccacct tgattcccac
     4561 gatggctccg gccgcgatca tgatgccgct aagttcgaag gaaatggtcc attccacgag
     4621 ccggtgtccc gccagggtgc cgggaatcgg gatcattccc gggagcctca gggccgcggg
     4681 ccaccacccc aaaaggtttt cccgggcgaa ggccaacccg gaacccaaaa cggcggacac
     4741 ggccagtctg cggccccgct tgagggcgtc ggctcccgca ccgtacaggg accgcagggt
     4801 gactgccgcg gcaatcccgc tgggaaactt caattgctcg atgttgatca tgtgacgctt
     4861 catgggaatg gcgaggatca ctcccaggag ggccagaaag atggtccaaa ggagaagaat
     4921 tccgaaagga agggaacgtc cctcgatcat gagcagggca ggcaccgcgc tcaccagcat
     4981 gctgcccgtc gagtagcccg cagagctggc ggtggactgc atacagttgt tttcgaggat
     5041 gctcagattg gatcgaaaca gcccgatctt cgagaggacc gtcccgacgg cgaaggacag
     5101 gatgcacgcc gtgatggcca ctcccacccc ccatccggtc ttgaggccga tgtagaggtt
     5161 ggacaggctc atgactccgc ccaggatccc gccgatgaac acggccctcg gggtcagttg
     5221 ggggatgtct ccccggtagt ggttttcaac ccactcccgt tccaaatcct cgggagtctt
     5281 cccccgggga tcgattccca ggaagggcgg ggcgggcggg gggccggtcg agtcgctcgg
     5341 cgcatgcccc ttttccccgt ggatcatacg gcactgcctc caatccggcg agagggacgg
     5401 cggttgcttc cgggtggaag ccgatgcttc ccatacggga tgacgggttt cgttccattc
     5461 cggcgggctt gtatactact cgaact
//
LOCUS       DAOOVC010000214         5413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8529_length_5413_cov_5.259052, whole genome shotgun sequence.
ACCESSION   DAOOVC010000214 DAOOVC010000000
VERSION     DAOOVC010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8529_length_5413_cov_5.259052"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLM79_16970"
     CDS             complement(<1..311)
                     /locus_tag="PLM79_16970"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOV88051.1"
                     /translation="MTEGLRRVFLDWPWIYRGVRSGLAILFLYAGAVKLLGPRAFARV
                     ISGYGLVPDEWLVLVAVGLPVLEVLAGVGLLFDVRGSLPAVFGMLVLFLGVLGFGILN
                     QL"
     gene            complement(696..1487)
                     /locus_tag="PLM79_16975"
     CDS             complement(696..1487)
                     /locus_tag="PLM79_16975"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88052.1"
                     /translation="MNPSESVPQEKPEEGRPFHLEGIALGRTFRVNGGSLQVLEGVRF
                     RISTGEFVCILGRSGCGKTTLLKVVAGFLSPSSGRVLINGRDVRGPGSDRCVVFQEDA
                     LFPWLTVKENVTFGLRYRKKDGPRGLPDVDRLIESVGLARFKDYLPREISGGMKQRVA
                     LARVLVLHPEVLLMDEPFASLDSQTRREMQELLIALWEQTGHTVLFVTHDIEEALLLA
                     DRVLVMDKSPGRIREEIPVALPRPRKPETAEFLGLKRKLLETIGD"
     gene            complement(1484..2287)
                     /locus_tag="PLM79_16980"
     CDS             complement(1484..2287)
                     /locus_tag="PLM79_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV88053.1"
                     /translation="MKAFLKFPNDWRWMEPWVLPGLLVLVWAVCGQAGLAPDYLIPRI
                     QEVGKTAWHYVAGGSPGPFAGRFLTDLKASLLRVGGGFALAVVLGIPLGVLSGRFAWV
                     NRGFSNLIHGLRAVPGITWLPLAVVWFGVGMKTTLFLVAMAAFFPIYMNTVEGVRRIN
                     PLWIDAGAMLGVTGAGALFRILLPGAMPTIVAGLRLGLGISWAYLVLGELTGVPDGLG
                     AAVMDARMLGRVDMIVVGMILIAVIGRSTDRLLVGMLKCLFKSAGRMSS"
     gene            complement(2284..3276)
                     /locus_tag="PLM79_16985"
     CDS             complement(2284..3276)
                     /locus_tag="PLM79_16985"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOV88054.1"
                     /translation="MKGQRAAILLSVLLLFLSGFFLPTATGAGTEPVRVGTWRTAQTL
                     QPFYYERFLSVPVRVFPFTNPADQKLSLLAGSLDLCGTTLALAIQSAARGEPVVVVAA
                     LCNKCSALVVARESTVRTPKDLRDKRIGYVPGTMHEILLRETLARAGLRPDKDVKLVR
                     IDFFDMGTALAGGAVDAFLSGEPFPALSEVKGYGRVLEYPYFDDAVGTINAGLLVTRQ
                     TVLGRPDLVQSLVTAHARATEYLKANPDEWLGKASQFGTDLAVWKRAAENIELTWEMD
                     ESYVQKARALGRRMQEFGIIEKQPDYAALFHQGFVTRAREDLGLPGPGDEETRP"
     gene            complement(3418..4431)
                     /locus_tag="PLM79_16990"
     CDS             complement(3418..4431)
                     /locus_tag="PLM79_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88055.1"
                     /translation="MTWRIDSRVEDLLKKGEDLAGLEVEEAVHLLKIRPGSREMYALM
                     ETADRISRRQFNGKGELHFHIGVNASPCPYNCRFCSLTVEAGVFTQSVEFGPERVLSW
                     AREGQKQGADALNLMSTGTFPLGKLLEIGRFLRDRVSTPLVANTRDLTHEEGEALLEA
                     GFVGAYHSVRLGEGRDTPFSVQKRIQTIRVLRDVGLRWMNCVEPVGPEHTPEEIAELM
                     VLARRYGATYSGVMRRINFPGSPMGDRGMITEMELARMVAVSRLVMGDRVKAHCTHEP
                     NVLSLIAGANLLFPEVGSSPRDRKADTGKGRGTDLSGCGRMLTETGWDPGMPSNCFLP
                     PRA"
     gene            complement(4428..5204)
                     /locus_tag="PLM79_16995"
     CDS             complement(4428..5204)
                     /locus_tag="PLM79_16995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOV88056.1"
                     /translation="MDRGRREETFLISTAAGGIPTAVHYPERLPAPVIVCCHGLFSAK
                     DSSKFVSIGEEWSRQGFVVFRFDFPGCGESRVYPGPDLLSGRVRDLHAVLEHVSTQPW
                     FDGRIGLLGSSLGGTIALLVAASGNHPVRALVCWATPFDLHRLKSAVEEWDVMKELYP
                     AGFSLGSPVTLESLSPVPGVLVLHGRQDESVPWQDAGEIYLRAEEPKRLVLLETGDHR
                     LLDPECRRLALKISRDWFVELGLRPGGEDRELVSGAGSTP"
     gene            complement(5185..>5413)
                     /locus_tag="PLM79_17000"
     CDS             complement(5185..>5413)
                     /locus_tag="PLM79_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88057.1"
                     /translation="YPALVNFLHKTLAPGGMIYLAKNLELPANRFFVELTRCFEFKQT
                     ICKVRNEGEVQEIALYAVRPKQGVSVGPGQA"
BASE COUNT         1074 a         1802 c         1605 g          932 t
ORIGIN      
        1 agctggttta ggattccgaa tcccaagacc cccaggaaca agacaagcat tccgaaaacg
       61 gcgggaaggc ttcctcttac atcgaacagc agacctaccc ccgcaaggac ctccagcacg
      121 ggaaggccga cggccaccaa gaccagccat tcatccggga ccaagccgta tcccgaaatt
      181 acccgggcaa aggcccgggg acccaggagc ttcacggccc cggcatacag gaaaaggatc
      241 gccaacccgg agcggacccc tcggtaaatc cacggccaat ccagaaacac cctgcgcaac
      301 ccctccgtca tgaacccttc cctgccggtg tccggttcag aaaagcacgc gtcccgtcgg
      361 ttccattttc atctgtccca ggagctgcca acggctccca tcggcgatcc aattgagccg
      421 ccgccctcga gggggccgat ccccgttccc ggcgatccgc ggcacatcct cgttaccccc
      481 tcgaatcgta gaaaagcccg atcaagtcaa atcgtttgtt tcgatgcatt cccactcatt
      541 tcattggacg gttcgatgag agaggtccgg gcttgacttt ggagcggcaa ataaactcgg
      601 aaggaacgaa aggaagccgt tgagggaggg gcaggcatcg aacccgatgc ccgttgaggc
      661 aagacctgcg atccgggttt ccgaacggcg cccggtcagt ccccgatggt ctccaggagc
      721 ttgcgcttta agccgaggaa ctcggcggtt tcgggtttcc tgggccgggg aagggccacc
      781 gggatttctt cccggatccg tcccgggctt ttgtccatga ccagaacccg atccgccagg
      841 agcagggctt cctcgatgtc gtgggtgaca aagagaacgg tgtggccggt ctgctcccac
      901 aaggcgatga gcagctcctg catttcccgc cgggtctggg aatcgaggga ggcaaacggt
      961 tcgtccatga ggaggacttc cgggtgcagg accaggaccc gggcgagggc gacccgctgt
     1021 ttcattcccc cggagatctc ccggggaagg taatccttga aacgggccag ccccacggac
     1081 tcgatcaatc ggtccacatc ggggagcccc ctgggcccgt ccttctttcg atacctgagt
     1141 ccgaacgtca cgttttcctt cacggtcagc cacgggaaga gggcatcttc ctggaatacg
     1201 acgcatcggt ccgaaccggg accccggaca tccctcccgt tgatgagcac tcggccggac
     1261 gatggggaca gaaatcccgc cacgaccttg agcagggtgg tcttgccgca cccactccgt
     1321 cccaggatgc agacgaactc tcccgtggaa atccggaaac gaaccccctc gaggacctgc
     1381 agagatccgc cgttgacccg aaaggtcctg cccagggcga tcccttccag gtgaaagggg
     1441 cggccctctt ccggcttctc ttgcggcacg gattccgacg ggttcatgaa gacattctcc
     1501 cggcactctt gaagagacac ttgagcatgc ctaccaggag tcgatccgtg gaccggccga
     1561 tgaccgcgat gaggatcatc cccaccacga tcatgtccac tcgacccagc atccgggcat
     1621 ccatgacggc cgcgcccaag ccgtcgggaa cccccgtgag ctctcccagg accaggtaag
     1681 cccaggatat gcccagaccc agccggagcc cggccacgat ggtgggcatg gctccgggca
     1741 acaggatgcg aaagagggcc cctgcccccg tcacacccag cattgccccg gcgtcgatcc
     1801 acagggggtt gatccggcgg accccttcga ccgtattcat gtagatgggg aaaaaggcgg
     1861 ccatggccac caggaagagg gtcgtcttca tcccgactcc gaaccagacg acggcgaggg
     1921 ggagccaggt gatgccgggc accgcccgga ggccgtggat gaggttgctg aaaccccggt
     1981 tgacccaggc gaaccgcccc gacagcaccc ccagggggat gcccaggaca accgccaggg
     2041 caaaccctcc tcccacccgc agcaggctcg ctttcaggtc cgtgaggaac cttcctgcga
     2101 agggccccgg gcttccgccc gccacgtaat gccaggcggt ctttcccacc tcttgaatcc
     2161 ggggaatgag gtagtcgggt gccaggcccg cctggccgca gacggcccag accaaaacca
     2221 ggagccccgg cagcacccag ggttccatcc accgccaatc gttcggaaac ttgaggaagg
     2281 ccttcatggg cgggtctctt catcccccgg ccccggcagt cccaggtctt ctcgagcccg
     2341 ggtcacgaaa ccttggtgga aaagagccgc gtaatccggc tgtttctcga tgatgccgaa
     2401 ctcctgcatt cgtcgcccca gggcccttgc cttttggacg taggattcat ccatctccca
     2461 agtaagctcg atgttttcgg cggcccgctt ccataccgcc aggtccgtcc cgaattgcga
     2521 ggctttcccg agccattcgt ccgggttggc cttcaggtac tcggtggccc gggcatgggc
     2581 cgtcaccagg ctctgaacca ggtccggccg cccaagaacc gtctgccggg tcaccagcaa
     2641 cccggcattg atggtcccca cggcgtcgtc gaaataagga tattcgagga ccctcccgta
     2701 acctttcacc tcgctcaggg ccggaaaggg ctctcccgaa aggaaggcgt ccacggcccc
     2761 accggccagg gcagtcccca tgtcgaagaa atcgatccgc acgagcttca cgtccttgtc
     2821 cgggcgcagc ccagctcggg ccagggtttc ccgtagaagg atttcgtgca tggtgccggg
     2881 aacatagcct atgcgcttgt ctctcaggtc cttgggggtg cggacggtgc tctcccgggc
     2941 gaccaccagg gcggagcact tgttgcatag ggccgcaacc accaccacgg gttccccccg
     3001 ggcggccgac tgaatggcca gggcaagggt ggtgccgcac aaatcgagac ttcccgccaa
     3061 caaggagagc ttctggtccg cgggattggt aaaggggaaa acccgaacgg gaacggagag
     3121 gaacctctca tagtagaacg gttgcagcgt ctgggccgtc cgccacgtcc ccacgcgtac
     3181 gggctccgtc ccggcacccg ttgccgtcgg gagaaaaaac cccgagagga agaggagcag
     3241 aacgctcaag aggattgccg cgcgttgtcc tttcatgatc gaaaccccct cccgcgaacc
     3301 cgaattccct tccacctttg ccgaaaaacc tccatgaacc ggtgctcggg attggcaaga
     3361 cggagcccat cctttggccg ggctgcatcc cgggagggcg gaaagaagga tcggaagcta
     3421 tgcacgaggt ggaaggaagc agttggaggg catcccgggg tcccagccgg tttcagtcag
     3481 cattcggccg caacccgaaa gatcggttcc acgccctttt cccgtgtccg ccttcctgtc
     3541 ccggggactg gaccccactt ccgggaacag caggttcgca ccggcaatga gagagaggac
     3601 gttgggttca tgggtgcaat gggccttcac cctatctccc atgaccagcc gactcaccgc
     3661 caccatccgg gccaactcca tctccgtgat catcccccgg tctcccatgg gggaaccggg
     3721 gaaattgatc cgccgcatga ctccgctgta tgtggccccg tatcggcgcg ccaggaccat
     3781 gagctcggcg atttcctcgg gagtatgctc gggccccacg ggttccacgc agttcatcca
     3841 cctcaacccc acgtcccgca acacccggat ggtttgaatc ctcttctgaa cggagaacgg
     3901 ggtgtcccgg ccttccccga gtcgaacgga atggtaggcc cccacgaatc cggcctcgag
     3961 gagggcctcc ccttcttcat gggtgagatc ccgggtattg gccaccaggg gagtgcttac
     4021 cctgtccctc aggaagcgcc cgatctccag caatttgccc agggggaacg tccccgtgct
     4081 catgaggttc agagcatcgg ccccctgttt ctgcccttcc cttgcccagg aaaggactcg
     4141 ctcgggaccg aactccacgg actgggtaaa gaccccggcc tcgacggtga gggaacagaa
     4201 ccggcagttg taggggcagg gggaggcgtt gaccccgatg tggaagtgga gctctccctt
     4261 gccgttgaat tggcgacggg agatccggtc cgccgtctcc atcagcgcat acatctcccg
     4321 ggatcccggg cgaatcttca agagatgaac ggcctcttcc acctccaggc ccgccaggtc
     4381 ctcccctttc ttgagaaggt cctccaccct cgagtcgatg cgccatgtca tggggtgctt
     4441 cccgctccgg agacgagttc tcggtcctct ccacccggtc gaagcccgag ctccacaaac
     4501 caatcccggc taatcttcag ggccaggcgt cgacactcgg gatccagcag ccggtggtcc
     4561 cccgtttcga gaaggacgag cctcttgggt tcttcggccc gcaggtagat ttcccccgcg
     4621 tcttgccagg ggacgctctc gtcctgccgg ccgtgaagaa ccaagacccc cggcaccggg
     4681 gaaagggatt cgagggtcac gggagaaccc agggagaaac ccgcagggta gagctctttc
     4741 atgacgtccc attcttccac ggcggatttc aaccgatgga ggtcaaaggg ggtcgcccag
     4801 cacaccagtg cccggacggg atggttgccc gaagctgcca ccagcaaggc gatggtgccc
     4861 cccaggctgg atcccaggag gccgattctc ccatcgaacc agggttgagt cgacacatgt
     4921 tccagcactg catgcaggtc ccgcaccctc cccgagagca ggtccggccc gggatagacc
     4981 cggctttcgc cgcagccggg gaaatcaaac cgaaacacca caaacccttg gcggctccat
     5041 tcctcgccga tggacacgaa cttgctgctg tccttggcac tgaacagacc gtggcagcag
     5101 acgatcaccg gggccggaag gcgctccggg taatgaacgg ctgtgggaat cccgccggcg
     5161 gcggtggata tgaggaaggt ttcttcacgc ctgccccggt ccaacggaca ccccctgctt
     5221 gggacgaacg gcatagaggg cgatctcctg cacctccccc tcgttgcgaa ccttgcatat
     5281 tgtctgcttg aattcgaagc accgggtcag ctcgacgaag aaccggttgg cgggcaattc
     5341 caggttcttg gccaggtaga tcatccctcc cggggccagg gtcttgtgaa ggaagttcac
     5401 cagggccggg tag
//
LOCUS       DAOOVC010000215         5399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8553_length_5399_cov_6.925150, whole genome shotgun sequence.
ACCESSION   DAOOVC010000215 DAOOVC010000000
VERSION     DAOOVC010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_5399_cov_6.925150"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLM79_17005"
     CDS             complement(<1..376)
                     /locus_tag="PLM79_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88058.1"
                     /translation="MHPMKRLPLFSTAVFLAAIFCLHSPAPAATEAFDLLTVSGAFLN
                     PQMKPVKEVEIQVLVNGRVVKPMGKDEHIFSGNNGAFTAQFQLAPGALPGAVVEIRAQ
                     KPSWEPLKPTALKVVESGVDDQG"
     gene            complement(454..1920)
                     /locus_tag="PLM79_17010"
     CDS             complement(454..1920)
                     /locus_tag="PLM79_17010"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOV88059.1"
                     /translation="MASGKMSVDWKRIFFIVLGLALFFGFYFMNDLADAVDPAGRHFP
                     LPSQGRMALGLFLMAAVWWIFEVMPIGATAIAIGLFQVIFGIRSSDQALKDFFDPSVW
                     FIFGSVVMGLAFAHSGLTKRMAYKMLGIVGENTNLILLGTFIIVAGMTLLMAHTAVAA
                     AMFPLLMAIHSLYSDSDKPTKFGKGLFVGMAWAAGAGSIITFLGSARGVAGAGMFKKF
                     TGGVEIGFFEFTWYNLPVGLLMVLFIWLVVIFCFKPERSRIEGLRERVRELARELGPM
                     NTKEKLVLVMVAVVLALFMMKSFSPIPFFKNMDRAAIMMVPTLVFFLAKTLGVEEMEG
                     IPWNIILLFGGAMSIGYCLYDTKAAEWMAVSWVSWFQHAPWLLFVLAIAFLVLCMTNF
                     IMNVAAIAITLPVSLVIANYLGVAPHVIFFASLAAAGMPFNLLIGAAPNAIAYESKQF
                     STGEFFLYGWIPSLILMALLAVFCYFVWPLMGMPVLLK"
     gene            complement(2202..2633)
                     /locus_tag="PLM79_17015"
     CDS             complement(2202..2633)
                     /locus_tag="PLM79_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88060.1"
                     /translation="MKIKVLLVDDEKEFTDTLAQRLEIRDFEVTTASSADEALAQLGK
                     REVDVVVLDVLMPGKDGVAALHEIKKVHPLVEVILLTGHATVESAVEGLKSGAFDYLL
                     KPTETKDLIAKIVQAYRRKADQEERIRQAHIDSIMLRRGWD"
     gene            complement(2689..3168)
                     /locus_tag="PLM79_17020"
     CDS             complement(2689..3168)
                     /locus_tag="PLM79_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88061.1"
                     /translation="MALKLLILHADDLFRRHLSERMRRENYLVFDASPADPVSEVLGR
                     SNFDVVLLGVPGQHRDSLALLKTIKEVRPFTEVILLTALEEHSLYRSIQAMQMGAFDD
                     LLVPLDVHALHGRIREAYQRKKERVKAMRATLRQRRAVRAAENPPVRKSAKKNPAES"
     gene            3626..5119
                     /locus_tag="PLM79_17025"
     CDS             3626..5119
                     /locus_tag="PLM79_17025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOV88062.1"
                     /translation="MNPPRNQHPWPGGMESPDLRAALPFILDEIPLGIAVMDSRRRVV
                     TLNRAMEALTGFDRKTARALACRHVLRSNLCVGRCPARDMNRGSGSVCLEGDLINRER
                     QKIPVRVTFTPIRDARGNDLGFFEAVEDIRMLCALAGKTEGAYAFGQLVGRSPRMEQI
                     YRFLPMIAQSDSSVLLTGETGTGKDLVAEAIHEGSPRAKGPFIKVNCGALPETLLESE
                     LFGHHKGAFTGAVENKPGRFRMAHNGTLYLTEIGDLPLSLQVKLLTFLDDRIVHPLGS
                     TKGFLCDVRVIAATHRDLEGMVRDGTFRQDLLYRLNVVRLQIPPLRERKDDVRLLLDH
                     FLHTFVSRFKKPLKGFSPEALEILLAYSYPGNVRELKNIVEYASNVCREDHIRPDHLP
                     AYLTSPIPGDPPQDEATPAAREPFPAVHEEREAPLDRAAMERRIILEAILKGREEKEE
                     LQWPAIERKMILDALVKARGRRNQAAAVLGWSRSRLYRKMKQHGIEV"
     gene            5177..>5399
                     /locus_tag="PLM79_17030"
     CDS             5177..>5399
                     /locus_tag="PLM79_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88063.1"
                     /translation="MAAKVLICLYGNEVAPRFDLATEVLMAFLEEDGRVHGERVVVLP
                     QASAETLCHMVLTENIQVVICGGIEEEYYQ"
BASE COUNT         1120 a         1662 c         1633 g          984 t
ORIGIN      
        1 ttccctgatc gtccaccccc gattccacga ctttgagggc ggtgggtttc aggggttccc
       61 aggagggctt ctgggctcgg atctccacca cggccccggg caaagccccc ggagccagct
      121 ggaattgggc cgtgaacgcg ccgttgttgc ccgagaaaat atgctcgtcc ttgcccatgg
      181 gcttcaccac cctgccgttt accaagacct ggatttccac ctccttcacc ggcttcatct
      241 gcgggttcag aaaagccccg gagacggtaa ggaggtcgaa ggcctcagtg gctgcaggag
      301 cgggggaatg gaggcagaaa atcgccgcca ggaataccgc cgtggagaaa aggggcaatc
      361 gcttcattgg atgcatggga ccgatcctct tacactttgg ttgagacacc gacttccgtc
      421 actcgacgac caacggacca ttccggacgg gcattacttc agcaagaccg gcatgcccat
      481 caacggccag acaaagtaac agaagaccgc cagcaacgcc atgaggatca ggctggggat
      541 ccaaccatag aggaaaaatt ctccggtgct gaactgtttg ctttcgtagg cgatggcgtt
      601 gggcgccgcc ccgatgagca ggttgaaggg catgcccgca gcggccaggg aggcgaagaa
      661 aatgacgtgg ggggccactc ccaagtagtt ggcgatgacc agggagacgg ggagggtgat
      721 ggcgatggcc gccacgttca tgatgaaatt ggtcatgcac aggaccagga aggcgatggc
      781 cagcacaaaa agcagccagg gcgcatgctg aaaccagctc acccagctca cggccatcca
      841 ttccgccgcc ttggtgtcgt agaggcagta gccgatgctc atggctccgc cgaaaagaag
      901 aatgatgttc caggggatgc cttccatttc ctccaccccc agggtcttgg ccaggaagaa
      961 aaccagggtc ggcaccatca tgatggcggc ccgatccatg ttcttgaaga agggaatggg
     1021 ggaaaaggac ttcatcatga aaagtgccag caccacggcg accatgacca ggaccagctt
     1081 ttccttggtg ttcatgggac ccagttcccg ggccagttcc cggaccctct cccgcaagcc
     1141 ctcgatgcgg gagcgttcgg gtttgaagca gaaaatcacg acgagccaaa tgaagagcac
     1201 catgagaagt cctacgggga ggttgtacca ggtgaattcg aagaagccga tttcgacccc
     1261 gccggtgaat ttcttgaaca tcccggcccc ggccactcct cgcgccgaac ccaaaaaggt
     1321 gatgatggaa cccgccccgg cagcccaggc catgcccaca aacaggccct tgccgaactt
     1381 ggtgggcttg tccgaatcgc tatagaggga atggatggcc atgagcaggg ggaacatggc
     1441 cgccgccacg gcggtgtggg ccatgagcag ggtcatgccg gcgacgatga tgaaagtccc
     1501 cagcaggatc aagttggtgt tctcccccac gatcccaagc atcttgtagg ccattcgctt
     1561 ggtgaggccg gagtgggcga aggcgagtcc catgaccacg gagccgaaaa tgaaccacac
     1621 ggaagggtcg aagaaatctt tcagggcctg gtcggagctc cggattccga agatcacctg
     1681 aaaaagcccg atggcgatgg ccgtagcgcc gatgggcatg acttcgaaaa tccaccagac
     1741 cgccgccatc agaaacagcc ccaaggccat cctgccctgg gacgggaggg gaaagtgcct
     1801 gcccgcaggg tccaccgcgt cggcaagatc gttcatgaag tagaagccga agaacagggc
     1861 gaggcccagg acgatgaaga agatcctctt ccagtcaacc gacattttcc ccgaggccat
     1921 gaccccctct tgggaccctt cttggagacc cgcgcccatc ggaatgcttc tcgtttcttc
     1981 taccatggct gccaactcct ttcaatcctt cgacattcac aacacgtacc ggatggatcc
     2041 aaccctcatt ggggccaaag gcgaaaccgg ccgcccggat tatgtgaagc agctcacatg
     2101 gccgagccaa agaaaaggca gtcatgattc cgctgggctt catgcctgcc cggccggtta
     2161 cgcggcggaa acgggccttg ggaaaactcg ccgcaacccg actaatccca tccccgccgc
     2221 agcatgatgc tgtcgatgtg ggcctgtcga atgcgctctt cctggtccgc tttccgccga
     2281 tatgcctgga cgatcttggc gatgaggtcc ttggtttcgg tgggcttcaa gagatagtcg
     2341 aaagccccgc tcttcaatcc ctcgacggcc gattccacgg tggcgtggcc ggtgagcagg
     2401 atgacttcca ccaggggatg aaccttcttg atctcatgaa gggcggcgac gccgtccttg
     2461 cccggcatca ggacatccag cacaaccacg tccacttccc gtttgcccaa ttgagccagg
     2521 gcttcgtcgg cgctggaagc cgtggtgacc tcaaagtccc ggatctcgag ccgctgagcc
     2581 agggtatccg tgaattcctt ttcatcgtcc accaagagca ccttgatctt catgaaccgt
     2641 tctccttggt tttcctgttg aattctttca ttccgtgctg catcctggtc aggattcggc
     2701 gggattcttc ttggccgatt tccgcacggg aggattttcc gcggcccgga cggcccgcct
     2761 ctgccgcagg gtcgcccgca ttgccttcac cctctccttc tttcgttggt aggcctcccg
     2821 gatgcgtccg tggagcgcgt ggacgtccag gggaaccagg aggtcgtcga aagctcccat
     2881 ctgcatggcc tggatggaac ggtagaggga atgctcttcc agggccgtca ggaggatcac
     2941 ttccgtaaag ggccggacct ccttgatcgt cttgagaagg gccaggctgt ctcggtgctg
     3001 tcccggcact cccaacagga ccacatcgaa gttgctgcgc cccaggacct cgctcaccgg
     3061 gtctgccggg gatgcatcga aaaccaggta attctcccgg cgcatgcgct cggaaaggtg
     3121 cctgcggaaa aggtcgtcgg cgtgaaggat cagtagtttc aaggccattg gcggcagcaa
     3181 tccgtcctgg ggcatcggtt ttttgggccc caaccgatgg cagccgggaa ccggcggatt
     3241 ccggttcggg aaaccccgtg ccgtgctctc aagaggccga ggcgatggga ggccgccccc
     3301 cctgggtctg gggccccctg ccggggagac ggacccttcg agggatttgc ctcgggaccc
     3361 ggtttttcct tgaggcaacg accgtgccgg ccggacctat ccagtaaatc cggccactta
     3421 ccgcgaccgg ttttggggtc tcccggaaaa gaagcggaag ggtgaaacat tatgatgcat
     3481 tatgattctt ttatgataca ttataataca gattgattca aggtttcgaa aagcctgggg
     3541 cggtcgtttc cgggaccccg aaaggacccc cgggaagctt gggccgaatc cccggggtcc
     3601 ccttgaggag agagaagaca agctcatgaa tcccccgagg aaccaacatc cgtggcccgg
     3661 cggcatggaa tccccggacc tgcgggcggc cctgcccttc atcctggacg aaatcccttt
     3721 gggcatcgcc gtcatggact cccggcggcg ggtcgtcacc ctcaatcgag ccatggaggc
     3781 cctcaccggg ttcgatcgca agacggcccg ggcccttgcc tgccggcacg tcctgcgcag
     3841 caacctctgc gtgggccgat gcccggcccg ggacatgaac cggggctccg gttccgtatg
     3901 cctcgagggg gacctcatca atcgggagcg ccagaagatt cccgtgcggg tcaccttcac
     3961 ccccatccgg gacgcccggg gcaacgacct cggttttttc gaggccgtgg aagacatccg
     4021 gatgctctgc gccctggcgg ggaagaccga gggagcctac gccttcggcc agctggtggg
     4081 tcgaagcccc cggatggaac agatctaccg gtttcttccc atgatcgccc aatccgactc
     4141 ctcggtcctc ctgacgggag agacgggcac ggggaaggac ctggtggccg aagccattca
     4201 tgaaggatct ccccgggcca aaggaccctt catcaaggtc aactgcgggg ccttgcccga
     4261 gaccctgctc gaatcggaac tcttcggtca tcacaagggg gctttcacgg gggcggtgga
     4321 aaacaagccc gggcgctttc gcatggccca caacggcacc ctgtatctca ccgaaatcgg
     4381 cgacctgcct ctgtccttgc aggtaaagct cctcaccttc ctggacgacc gaatcgtcca
     4441 tcccctgggg agcaccaagg gcttcctgtg cgatgtgagg gtgattgccg caactcaccg
     4501 ggacctggag ggcatggtga gggacgggac cttccggcag gacctgcttt accggctcaa
     4561 cgtggtgcgc ctccagatac cccctttgcg ggaacgcaag gacgacgtcc ggctccttct
     4621 ggaccacttc ctgcacacct tcgtctcccg gttcaaaaag cccctcaagg ggttttcccc
     4681 cgaggccctg gaaatccttt tggcctactc ctatccgggc aacgtgcggg agctcaagaa
     4741 catcgtcgag tacgcatcca acgtgtgccg ggaggatcac atccgccccg accaccttcc
     4801 cgcctacctc accagtccca tccccgggga ccccccgcag gacgaggcaa ccccggccgc
     4861 ccgggaaccg ttcccggccg tgcacgagga aagggaggcc cctctcgacc gcgcggccat
     4921 ggaaagaaga atcatcctgg aggccatctt gaaaggtcgg gaggaaaagg aagagctgca
     4981 gtggcccgcc atcgagagga agatgatcct ggacgccctt gtgaaggccc gggggcggcg
     5041 gaaccaggcg gcggccgtgc tcggctggag ccgaagcagg ctctacagga agatgaaaca
     5101 acacggcatc gaggtttgaa gggttccggt cccgtccggg aaaaagcctc gaccgcccac
     5161 gactcgaggg gatgaaatgg cggccaaagt cctcatctgc ctgtacggca acgaagtggc
     5221 cccccggttc gatctggcca cggaggttct catggccttt ctcgaggaag acggaagggt
     5281 gcacggggaa agagtggtgg tgctgcccca ggcctcggcg gagaccctct gtcacatggt
     5341 cctcaccgag aatatccagg tggtcatctg cggcgggatc gaggaagaat actaccaat
//
LOCUS       DAOOVC010000216         5246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8871_length_5246_cov_16.574456, whole genome shotgun sequence.
ACCESSION   DAOOVC010000216 DAOOVC010000000
VERSION     DAOOVC010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_5246_cov_16.574456"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLM79_17035"
     CDS             <1..388
                     /locus_tag="PLM79_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV88064.1"
                     /translation="CNHCDNPPCVYNCPTGASHKRKDGTVQVDRDKCIGCKACVAACP
                     YNARYMDPKEGYADKCTFCEHRVREGREPACVATCIGGSRIFGDLDNPKSAVSRILKE
                     VSAHVLLKEAGTQPRVFYVRRLSRPE"
     gene            398..1354
                     /gene="nrfD"
                     /locus_tag="PLM79_17040"
     CDS             398..1354
                     /gene="nrfD"
                     /locus_tag="PLM79_17040"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOV88065.1"
                     /translation="MEITITGTNALTYPSLHIWNWMVAVDLFFGGLAAGLLIMSAIVN
                     LTEGKDKVACVKGPLVAPFCLMIGAFFIWLDLERPFNFFQIMFTFQPSAPMSWGAWGI
                     TLIIPLSFLYGLSTVPAEYKHWLRFEFLKKLSDRLNPYMKPLARINFVLGVFLGIYTG
                     ILLSLFVARPLWNSALLPLVSLSSSIATGAALSAIIARRITSKIFFSRAVVWLLGVQL
                     VIIALFFFSHLTSTAPQRESVMPFFSFTNEYFWYGASTVFFALLLPLGLVLGLVRTKK
                     DYGLEPSGAALLRLKVSSFMVLIGGLIIRLAILYAGQLSKLA"
     gene            1521..3413
                     /locus_tag="PLM79_17045"
     CDS             1521..3413
                     /locus_tag="PLM79_17045"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOV88066.1"
                     /translation="MSPLVQILGQEKAFPADENATLLDVLLATGFSVSADCGGKGRCG
                     RCKVKVVEGATELPPSQTEMRRLSEEELAAGFRLACQQKVAEGLVLEVVEEIYRREVY
                     KRLGIGIGKPLPLAPHVRKVPLPEGKGLEDAFEESGLSGDIDWAAVLSDAVDPRFLRD
                     FDPDPSDSRPPHYQTALTNDSGRVIGLCRPDTELHGIAVDLGTTTIAVYLCNLERGEI
                     LGVETARNPQVSYGADVMSRIKASQDPQNAAHLQELARKTINSCITTISRRHELPLKS
                     LVDAVVVGNPTMIHLLLGAHAQGLAVAPYRPIFHSSLAVRARDLGFSLHPGAAVHTLP
                     LPSAFVGADTVAAWLWAEREIEDRSTLLLDLGTNGEMVLSAKGGLWATSCATGPAFEG
                     ATLTCGMAGIPGAVERVHFKAARLVLKIIGSSTDPRIRPRGICGSGAMSVLAALLQEE
                     ILRPDGGFEIASGHKLLQSTPRGVHFVLAPAKESASGKPVVLHQKDIRDLQFAKGAVA
                     TGIRFLCDAAGIDAPERILLAGAFGNVIDPEDALTIGLIPEVPRENIRGIGNAAGLGA
                     CKALLDIRARKRARDLLREIRVVELGGAPGFKEAFFANLSFPRPDTTLRKDRSVQPRP
                     GKSSPS"
     gene            3410..3835
                     /locus_tag="PLM79_17050"
     CDS             3410..3835
                     /locus_tag="PLM79_17050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV88067.1"
                     /translation="MKRMFLLCMLLGAAFSAPHTQADFEAGMKAAEEGALADAFEEWM
                     VDAQQRGDPRSQFQLGLVFDRGLIGLQDDHEAAKWYRLAADQGHAEAQYRLGLLYYFG
                     QGVEQNHEQALRWFQKASEQGNENAGVFLRTMGSKCDFP"
     gene            complement(4061..4252)
                     /locus_tag="PLM79_17055"
                     /pseudo
     CDS             complement(4061..4252)
                     /locus_tag="PLM79_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
     gene            4410..4520
                     /locus_tag="PLM79_17060"
     CDS             4410..4520
                     /locus_tag="PLM79_17060"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOV88068.1"
                     /translation="MGRFDRITQQPEVMGGKACIRGMRVTVGMDLADTHR"
     gene            4536..5093
                     /locus_tag="PLM79_17065"
     CDS             4536..5093
                     /locus_tag="PLM79_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOV88069.1"
                     /translation="MLIGYARVSTHDQNLELQRKALTMAGCQKIYEDKVSGARAERPG
                     LTKALEMLREGDTLVVWKLDRLGRSVKQLVDLIGELHKQAVQFKSLTDAIDTGSPSGR
                     FFFHVMAALAEMERELTVERTRAGLEVARQLGRKGGRKPKMTDSKIESAKKLLASGIP
                     PRDVAKNLGISVPTLYRWVPASAHA"
BASE COUNT         1082 a         1550 c         1494 g         1120 t
ORIGIN      
        1 ttgcaaccac tgcgacaatc ctccttgtgt gtacaattgt cccaccggag cttcccataa
       61 gagaaaagac ggaacggtcc aggtcgatcg agacaagtgc atcggctgca aagcttgcgt
      121 tgccgcatgc ccttacaatg cccgctatat ggaccccaag gaaggatacg cggacaaatg
      181 caccttctgt gaacacaggg ttcgggaggg gagggagccc gcgtgtgtcg caacctgcat
      241 tggaggctcc cgcatttttg gggacctcga caatcccaag agcgcagtca gccggatcct
      301 caaagaggta agcgcccatg tcttattgaa agaggccgga acccaacctc gggtattcta
      361 tgtcagaaga ctgagcaggc cagaatagga gcttctcatg gagatcacaa tcacaggaac
      421 caatgctttg acttacccga gcctgcatat ctggaattgg atggttgcgg tggatctctt
      481 ttttggggga ctcgctgcag gcttgctgat catgtcggcc atcgtcaacc tcaccgaggg
      541 gaaagacaag gtggcttgcg tgaaaggtcc cctggttgca cctttctgcc tcatgatcgg
      601 tgccttcttc atctggttgg atcttgagcg gcccttcaac ttttttcaga tcatgttcac
      661 cttccaacca agcgctccca tgtcctgggg cgcatgggga atcaccctga tcattccttt
      721 gagcttcctc tacggccttt ccacggttcc ggcggagtat aagcactggc tgagattcga
      781 gtttctcaaa aagctctccg atcgtttgaa cccgtacatg aaaccccttg cccggatcaa
      841 cttcgtcctg ggggttttcc tcggcattta tacggggatt ctcctcagcc tctttgttgc
      901 gagaccccta tggaattccg ccctgctccc gctggtcagc ctgagctcct ccatcgccac
      961 gggggcggcg ttgagcgcca tcatcgccag gagaattacc tcgaagatct ttttctcaag
     1021 ggcggtggtt tggctcctgg gcgtccaact ggtgattatt gctcttttct tcttctctca
     1081 cctcacgtcc actgctcccc agagggagtc ggtgatgcct ttcttttcgt tcaccaacga
     1141 gtatttctgg tatggtgcct ccacggtttt ctttgctctg ctgctgcctc tggggttggt
     1201 tctgggactc gtccggacca agaaagatta cggactggaa ccttccggtg ccgcgttgct
     1261 tcgcctgaaa gtcagctctt tcatggtgct cataggaggt ctcattattc ggcttgccat
     1321 tttatacgcc ggccagttga gcaagttggc ctaacagaag ggcgatacgg gactcaatcc
     1381 tgcacggcct gctcgaagaa cggccggagc cgtgcaaaac ggctcccatc cgagaagggc
     1441 atcgcttttc gcatgtcgtc atcctctcgc ccgtccgatt gagttttcca aatgctcaga
     1501 caaaacaggg aggggaattt gtgagcccgc tggttcagat cctggggcag gaaaaagctt
     1561 ttcccgccga tgaaaatgca acccttctgg atgtgctcct tgccacaggg ttttcggttt
     1621 ccgccgattg cgggggaaag gggaggtgcg gccggtgcaa ggtcaaagtc gtggaaggag
     1681 caacggaact cccgccctcc caaacggaaa tgcgccgcct gagtgaagaa gaacttgccg
     1741 cgggctttcg attggcctgt caacagaagg tcgcggaagg tctcgttctg gaggtcgtcg
     1801 aggagatcta tcgccgggag gtgtacaaga ggctgggaat tggaatcggg aagcccttgc
     1861 ccctggcacc ccatgtccgg aaggtcccgt tgcccgaagg aaaaggtctg gaagacgcct
     1921 tcgaggaatc gggcctttcc ggggatatcg actgggccgc agtgctttcc gatgcggtgg
     1981 atccccgttt tcttcgagat ttcgatcccg acccctccga ttcccgtccc cctcactacc
     2041 aaaccgcatt gacaaacgat tccggaaggg tgatcggcct ttgccgtccc gacaccgaac
     2101 tgcacgggat cgccgtggac ctgggaacca caaccattgc ggtctatctg tgcaacctcg
     2161 agagggggga gattctcggg gtcgagacgg cacgaaaccc ccaggtctcc tacggtgcgg
     2221 acgtgatgag ccggatcaag gcctcccagg acccccagaa cgccgcgcat ctccaggagc
     2281 ttgcccgaaa gaccatcaac tcttgcatca ccaccatatc ccgaaggcat gagttgccct
     2341 tgaagtcatt ggtggacgca gtcgtggtcg ggaaccccac catgatccat ctcctcctgg
     2401 gggcccacgc ccaaggcctg gccgtcgctc cataccgtcc catttttcat tcgagccttg
     2461 cggtccgagc cagagacctg ggtttctctc tccatcccgg cgccgcggtc cacacgttgc
     2521 cccttccttc cgcctttgtc ggggcggata ccgtggccgc atggctgtgg gcggaacggg
     2581 agatcgagga tcgatccacc ctgctcctcg accttgggac caacggagag atggtattgt
     2641 ccgcaaaggg cggcctgtgg gccacctcct gcgccaccgg acccgccttc gagggggcca
     2701 ccctcacctg cggcatggcc ggcatcccgg gagccgtcga gagagtacat ttcaaagcag
     2761 caagactcgt tttgaagatc attgggagct caacggatcc gcgtattcgt cctcgaggga
     2821 tttgcgggtc cggagccatg tcggttctcg ccgctcttct ccaagaagaa atcctccgcc
     2881 ccgacggtgg gttcgagatt gcctccggac acaagctcct tcaatcaacc ccccggggag
     2941 tccactttgt cctggctccc gccaaagagt ccgcatcggg aaagcccgtg gttctccacc
     3001 agaaggatat ccgggatctg cagttcgcaa aaggcgccgt cgcaaccggc atccggttcc
     3061 tttgcgatgc cgccgggatc gatgctcccg aaagaattct ccttgccggg gccttcggaa
     3121 acgtgattga tcccgaagac gccttaacca ttggcctgat tcccgaggtc ccccgggaaa
     3181 acatccgggg gattggaaat gcggcggggc tgggtgcctg caaggccctc ctcgacatcc
     3241 gcgctcgaaa gagagcccgg gacctcctgc gggaaatccg ggtggtggaa ctgggaggag
     3301 ctcccggatt taaagaagcg tttttcgcca acctttcctt cccccgtccg gataccaccc
     3361 tccggaagga ccggtccgtt caaccacgcc ccggaaagtc ctccccctca tgaaacggat
     3421 gttcctcctc tgcatgcttc tgggggcggc gttctcggca ccccatacgc aggcggattt
     3481 tgaagcgggg atgaaggccg ccgaggaagg agccctcgcc gacgccttcg aggaatggat
     3541 ggtcgatgcg caacaacggg gagatccccg atcccagttc caactgggac ttgtgttcga
     3601 tcgaggactg atcggcttgc aggatgatca cgaagcagcc aaatggtacc ggctcgccgc
     3661 cgaccaggga cacgccgaag cccagtaccg cctgggactt ctctattatt tcggccaggg
     3721 cgtggagcaa aatcacgaac aggccctcag atggtttcaa aaggcttcag aacaggggaa
     3781 cgaaaacgcc ggggtctttc ttcgcacgat gggcagtaaa tgcgattttc cctaaattta
     3841 tgcccttaaa acacccttcc gggggtgact tcagaaaacg gatcatagag tacgtcaagg
     3901 ggaggggccg tagcgatctg aatttccctg ggccggcctt ggtgctattg cgccggaagg
     3961 aatcgccggt cggattgatc tgctcccacc ccagtgggga caggaattgc aaaagcccgt
     4021 catcgatgtt ctggccatga tcccgcagcg caatggccga catcacacct ggatagcgca
     4081 tgtagcaacg ctgcacccca aaccccaagt gatttgcagc gccgcgccgt tggcggatga
     4141 tggcgagatc ggcttcactg aaggcataat agcggatcag gtatgccttg gtattgggca
     4201 tcgccggcag gcttttccgc tcggtgccgg acagaatgca acgacacggc atgcatcttc
     4261 ctgcttctta taaacctggg tttttgataa gcggtgcggg tgcgctgttg cggcacgcaa
     4321 aatcaggacg ttgccattct cgataatagc tcttgaaatg ctattctcat ccatccagca
     4381 tttcaatgag tttcgataag gagagaccca tgggacgttt tgatcgcatc acccagcagc
     4441 ctgaggtaat gggaggaaag gcgtgcatcc gcggcatgcg ggtcacggtc ggtatggatt
     4501 tggcggacac gcatcgataa cgcgaggaac aggccatgtt gatcggttat gcgcgcgttt
     4561 caacccatga tcagaatctt gaacttcagc ggaaagcctt gaccatggct gggtgccaaa
     4621 agatctatga ggacaaagtg agcggcgccc gagccgaacg ccccggtctg accaaggcac
     4681 ttgaaatgct gcgcgagggg gacacactcg ttgtctggaa attggatcgc ctgggccgca
     4741 gcgttaagca actggttgac ctgatcggcg aactgcacaa gcaggctgtc cagtttaaga
     4801 gcctcaccga cgccatcgac accggcagcc catccggccg gttcttcttc catgtcatgg
     4861 ccgctctcgc cgaaatggaa cgcgagctca cggtcgaacg cacccgcgcc ggactggagg
     4921 tggcccgtca gcttggtcgc aaaggtggcc gcaagcccaa gatgaccgac agcaagatcg
     4981 agtcggccaa gaagttgctg gccagtggca ttccgcctag ggatgtagcc aagaacctag
     5041 gcatatccgt ccccactctg taccgctggg ttccggcctc ggcgcacgct tagcgtactc
     5101 tgtaatctgt ttttcgagac gaccccttcc ggaaaaaccc accgctcccc cccccaacac
     5161 ggctttcgga tttttcccca tgaaaaatta ccccccctcc cccttgggag gaaggggaaa
     5221 ggggggcggg ctggtgggga acacca
//
LOCUS       DAOOVC010000217         5229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_8909_length_5229_cov_7.536915, whole genome shotgun sequence.
ACCESSION   DAOOVC010000217 DAOOVC010000000
VERSION     DAOOVC010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8909_length_5229_cov_7.536915"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PLM79_17070"
     CDS             <1..369
                     /locus_tag="PLM79_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HOV88070.1"
                     /translation="PSLMRGRRDPRTLYVGDALDFWRVLEVSAPFRLLLLSEMKLPGE
                     AILEFTISPGGQGECELRQVARFLPKGTVGLLYWYSLYGAHQLLFKGMLRTIAREVGK
                     PIVKGPERFEPRLDLVCRID"
     gene            786..>5229
                     /locus_tag="PLM79_17075"
     CDS             786..>5229
                     /locus_tag="PLM79_17075"
                     /inference="COORDINATES: protein motif:HMM:NF014401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FYVE zinc finger domain-containing protein"
                     /protein_id="HOV88071.1"
                     /translation="MSPMTLEQLKGRMADNREFQDYLKWQESRSGRKILGDVALLRAY
                     QGYLLEHLGDVRGQLMDESDLRLSLVLALHFNPLFQQEFRAGSPTLAQRYDKETAVQS
                     SGPVNELMAQVFKQYAAWLTRELDDTMFRLEEAGTDEVLQYEKRRALELRFGKLFDRE
                     LRLAKEEGYTLQLAVQEKEQTKKEVMEFLSQDASSRVTRFNWAIFGNSDKTSAGIDLL
                     NREITRCNRVSGIMRAMLPRYAARPKSREILETQVALAESRRTEAEHYIARLKECKRV
                     KKQARISWQEKTAESEALLKQTDVELLGVSEDLLRGVRQVVPSLREQEAQIGTRLKEL
                     EKTVREIPRLYEEQEKQTNELVRIESILAGDPIRLKYLGNLGFKAGSLPALRRSIAER
                     ARLIEETELRQRHLSEDLAFLNAKYNAMFDRTEAALNDQQELNRLRAKAHDELQWLQS
                     LPRIHRALNVYCSTATVTFELFLSIGQIAAGRACGPVGGTAVAAMGIAGMPITYGLSL
                     PLSGALLTNIQPYDTGQLGISLKLGFSWGADFQPATKDSLEASVGMAIVYDASLQVSD
                     TRGFSTVSTITLATTAEAAIPGALRAAIEMELIKEKRVMTFKDVYHWAAWIGQKWANL
                     RAWAVAARLYRGGGTSQPTAEDLKRLREVADISLKNDKPTRDMLEKVFKYMGEPILRT
                     ECKELFPHIGAEISAAEFFTLKGARERRSEPKFIRRNSDIKTGEVYDEERKGDQWSRS
                     LTVGAGIQVQADYSNVEKDPNPDNVGESLTFTISLPIFDQNAQWISTPAMDPSKGAIG
                     TWVQSHLAPAANKISNFTNPGVFNLFKSSLATNPFLSLSTGLALGTVQVALFLSTLTK
                     RPAPDGKTGKWVLFYWRPVFSTATEIQRTVPLGYGFNMVLGGSVSLSRTYREQLGDNT
                     IVYLQIVYNGLMNISLPGDDGNPRPPEARGPALWKEYVDTQKKTIWRMMHNITLPHTW
                     IAAEVNDREGGAKLIADLKRIMPAPARRSSKGLPSQHKAPYVDVDAPESYPDFSQTLF
                     DKAFDPFQKHLEDFRKSDYLTHKNKDWHRIPIQGGTFNWSMNPIVIAGELYRTHTTQY
                     KLEKGVVDARTALGHRKGASQQVLADVKMVPDEQAPNCMLCGVAFSFIRRRHHCRECG
                     KVFCADCSSHSIELPHRSQFKPVRVCNTCYARLTAAPKQPAQAPSGHKDPGAADHGEP
                     LPRVQDPVSQPHREGFPAATHSPVSHPQKTGMLTSAYVSSQVPGKESLLTSSRRSPEA
                     PRGESPLSSSGSNLGALRGDSGRAQAPVVRGGEVRSEWGEGKDEITVLDEMEHERWWS
                     TRIARPVPSSADRQAYIDFAVSDAQAAQAMREESPASREAQKSKGKISPGGRSSRLKG
                     VTVAEEGRNPLVGKELTNYFDITDVSADGNCLFRSMAVALQRPENQHKAYRRLAVEHI
                     FRHTADFETVYQGNVPAYLYSMSETARYPEDKARWGGSPEILALS"
BASE COUNT         1103 a         1649 c         1586 g          891 t
ORIGIN      
        1 cccagcctca tgcggggaag gcgggatccc cggacgctct atgttggcga tgccctggat
       61 ttctggcggg tgctcgaagt ctccgcccct ttccggcttc tgctcctctc ggaaatgaag
      121 ctcccgggcg aggcgatcct cgagttcacc atctctcccg ggggacaggg ggaatgcgag
      181 ctccggcagg ttgcgcgctt tctgcccaag ggcaccgtcg ggcttctcta ctggtacagc
      241 ctgtacggcg ctcaccagtt gctcttcaag ggaatgcttc gcaccattgc ccgagaggtt
      301 ggaaagccaa tcgtgaaagg acccgagcgg ttcgaacccc ggttggatct ggtctgtcgg
      361 atcgactgat cctccgggtg cctcgatgcc gaaggtcccg aaggggcggc agccaaggcc
      421 gcggacgccg tcccgagatt cagcgggcga taaggatgtg ccggcggcaa gcgggcgggg
      481 caccccggga ggcgacatgg gtcggcctca acgtccccaa cacctgccga aactccctct
      541 ccggaaaacg cccggcacga tcgaggatgt gcttgacggg agcttcggcc caaagtatca
      601 gagtcagtgc tttcgttccg taccacacag accccccgag ggggagggcg tttccgcatc
      661 ccctcctccg cttgtctcct aacagcaacc gtaccgatcc gaaaaattct cctttttttc
      721 gagtcgtctc gggagcgttg tcccttctta cggccacaca ctgcgaagca gccaaaggat
      781 gatctatgag ccccatgacg ctggaacagt tgaaagggag aatggcggac aaccgcgaat
      841 tccaggacta tttgaagtgg caggaaagcc ggtcgggccg gaagatcctg ggcgacgtgg
      901 ccctgctgag ggcctaccag gggtacctcc tcgagcactt gggcgatgtc cggggccagc
      961 tcatggacga gtccgacctt cgcctctccc tggtgctggc cctccatttc aacccgctgt
     1021 ttcagcagga attccgggcc ggaagtccca ccctcgccca aaggtacgac aaggaaacgg
     1081 ccgttcagag ctccggaccg gtcaacgagc tcatggccca ggttttcaag caatatgcgg
     1141 cctggctgac ccgggaactg gacgacacca tgttccggct cgaggaggcc gggacggacg
     1201 aagtcctcca gtacgagaaa cgacgggcgt tggagctgcg gttcggaaag ctcttcgacc
     1261 gggagcttcg cctggcaaag gaggaaggct acaccctcca gctggccgtc caggagaagg
     1321 aacagaccaa gaaggaggtc atggagttcc tctcccagga cgcttcttcg cgcgtcactc
     1381 gatttaactg ggcgatcttc ggaaacagcg acaagacctc ggcgggaatc gacctcctca
     1441 atcgggagat cacacggtgc aacagggttt ccgggatcat gcgcgccatg ctgccccgtt
     1501 acgccgcccg cccgaaaagc cgggagatcc tcgagaccca ggtcgccctg gccgaatcgc
     1561 gccggaccga ggccgaacac tacatcgccc ggctcaagga gtgcaagagg gtcaagaagc
     1621 aggcccggat ctcctggcag gagaagacgg cggagtccga agccttgctg aaacagacgg
     1681 acgtggaact cctcggcgtg agtgaggacc tcctgcgggg ggtccggcag gtggttccgt
     1741 ccctgcggga acaagaagcg cagatcggga ctcgcctcaa ggaactggag aagaccgtcc
     1801 gggagattcc ccgtctctat gaggagcagg aaaagcagac gaacgagctc gtgcgaatcg
     1861 agtccatcct ggcaggggac cctatccggt tgaagtacct tggcaacctg ggattcaagg
     1921 ccggctccct cccggccctt cgccggtcca tcgccgagag ggcccggctc atcgaggaga
     1981 ccgagctgcg ccaacggcac ctgagcgagg atctcgcctt tctcaacgcc aagtacaacg
     2041 ccatgttcga tcggacggaa gcggccctca acgaccagca agagctcaat cggcttcggg
     2101 ccaaggccca cgacgagctc cagtggctcc agagccttcc ccggattcac agggccctca
     2161 atgtttactg ttccacggcc accgtgacct tcgagctttt tctgagcatc ggccagatcg
     2221 ctgcgggccg ggcctgcggt cccgtcgggg ggacggccgt cgcggccatg ggcatcgccg
     2281 gcatgcccat cacctatgga ttgagccttc ctctttcggg ggcgctcctc accaacatac
     2341 agccctacga cacggggcag ctgggcattt ccctcaaact gggcttctcc tggggtgcgg
     2401 acttccagcc ggccaccaag gacagcctgg aagcgagcgt gggcatggcc attgtctacg
     2461 atgcgtccct ccaggtgtcc gacacccgcg ggttctcgac ggtgagcacc atcaccctgg
     2521 ctacgacggc ggaggccgcc attcccggcg cccttcgggc agccatcgag atggagctca
     2581 tcaaagagaa acgggtgatg actttcaaag atgtctatca ctgggcggcc tggatcggcc
     2641 agaagtgggc caacctccgc gcctgggcgg tggcggcccg gctttaccgg ggaggaggga
     2701 cctctcaacc gacggcggag gacctcaagc gccttcggga agttgccgac atctccctca
     2761 agaacgacaa gccgacccgg gacatgctcg agaaggtctt caagtacatg ggggaaccca
     2821 ttctgcggac ggagtgcaag gagctctttc cgcacatagg agcggaaatc tcggcggcgg
     2881 aattcttcac tctcaaagga gcccgggagc gcaggtccga gcccaagttc atccggagga
     2941 acagcgacat caagacgggg gaagtatacg acgaagagag aaaaggggat cagtggagcc
     3001 gatctttgac cgtgggagcg ggaatccagg tgcaggcgga ttattccaat gttgaaaagg
     3061 accccaatcc cgacaacgta ggggagtccc tgaccttcac catctccctg cccatattcg
     3121 atcagaatgc ccaatggatc tcgaccccgg caatggatcc ctcgaagggg gccatcggca
     3181 cctgggtgca gagtcacctg gcgcctgcgg ccaacaagat atcgaacttc acgaatccgg
     3241 gggtgttcaa tctcttcaag agttccctgg ccacgaatcc tttcctgagt ctcagcaccg
     3301 gcctggccct gggcaccgtc caggtagccc tctttctttc gaccctcacc aagcgtccgg
     3361 cccccgacgg caagaccggc aaatgggtcc ttttttactg gcgccccgtt ttcagcacgg
     3421 ccaccgaaat ccagaggacc gttcccctgg gctacgggtt caacatggtg ctgggaggaa
     3481 gcgtgtcctt gtcccgcacc taccgggaac aactggggga caacaccatc gtgtacctcc
     3541 agatcgtgta caacggtctc atgaatatct ccctgcccgg cgacgacggc aatcccagac
     3601 cccccgaggc ccgcggtccg gcgctctgga aggaatacgt ggacacgcaa aagaagacca
     3661 tttggaggat gatgcacaac atcacccttc cccatacctg gattgccgcc gaggtgaatg
     3721 accgggaagg aggcgcgaaa ctcatcgccg acctcaagag aatcatgccc gcgcccgccc
     3781 gccgttcctc caaggggctg ccctcccagc ataaagctcc ctacgtggac gtggacgccc
     3841 cggagtccta ccccgacttc agccaaacgt tgtttgacaa ggcttttgac ccctttcaaa
     3901 agcacctcga agattttcgc aaaagcgatt acctcaccca caagaacaaa gactggcatc
     3961 gcatccccat ccagggaggc accttcaatt ggtccatgaa tcccatcgtc attgccgggg
     4021 aactctaccg aacccacacg acccagtaca agctcgagaa gggcgtggtg gacgcaagaa
     4081 ccgccctggg tcatcgaaag ggggcctccc aacaggtcct ggcggacgtc aagatggtgc
     4141 ccgacgagca ggcccccaac tgcatgttgt gcggggtcgc cttcagcttc attcgccgtc
     4201 ggcatcactg tcgcgaatgc gggaaggtct tttgtgccga ttgctcgagc cacagcatcg
     4261 agcttcccca caggagccaa ttcaaacccg tacgggtctg caacacctgc tatgcgcggc
     4321 tcaccgcggc gcccaagcag cctgcccagg ccccgagcgg ccacaaagac cccggcgccg
     4381 ccgaccatgg ggaacctctc ccgcgggttc aggatccggt gagccaaccc cacagggaag
     4441 gtttccccgc agcgacccac agcccggtga gccatcctca gaagacgggg atgttgacat
     4501 cggcctacgt ttcctcgcag gttcccggaa aggaaagcct gctcacgtcc tcccgccgaa
     4561 gcccggaggc tcccagaggg gaaagcccgc tctcatcgtc tggttccaac ctgggagcac
     4621 tccggggaga cagcggcagg gcgcaggccc cggtggtgcg gggaggtgag gttcgatcgg
     4681 agtggggaga ggggaaagac gaaatcaccg tgctggacga gatggagcac gagcggtggt
     4741 ggagcactcg gatcgccagg cccgttccca gcagcgcgga ccgccaggcc tacatcgact
     4801 tcgccgtttc cgatgcgcag gcagcccagg caatgagaga ggaaagcccg gcaagccggg
     4861 aggctcagaa aagcaagggc aaaatctctc ctggcggcag gtcctcgaga ctgaaaggag
     4921 tgacggttgc cgaagaagga aggaatccct tggtcggcaa ggagttgacc aattacttcg
     4981 acattacgga tgtctcggcg gatgggaatt gcctgtttcg atccatggcc gtcgctctgc
     5041 aaaggcccga gaatcagcac aaggcctatc ggcgcctggc ggtggaacat atcttccgac
     5101 acacggcgga ttttgagacc gtgtaccagg gcaacgtccc ggcctatctt tacagcatgt
     5161 cggagacggc acggtacccc gaagacaagg cacggtgggg tggttccccg gaaatcctgg
     5221 ctttaagcc
//
LOCUS       DAOOVC010000218         5153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9056_length_5153_cov_5.915849, whole genome shotgun sequence.
ACCESSION   DAOOVC010000218 DAOOVC010000000
VERSION     DAOOVC010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9056_length_5153_cov_5.915849"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PLM79_17080"
     CDS             complement(<1..638)
                     /locus_tag="PLM79_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOV88072.1"
                     /translation="MADALISPGVGAAMWGASAALTLYSAAKVKKDPDERKAPLMGVL
                     GAFIFAAQMINFSIPATGSSGHLGGGMILAVLLGPHAAFLTMASILTIQAFFFADGGI
                     LALGCNVLNLGFFPCFVAYPLLYRKIVSGRPTPGRVLAGASVASIVGLQLGALSVVIQ
                     TTLSGISELPMDTFLLLMQPIHLAIGVIEGLVTAAVLNSVGKARPDLLAEAHG"
     gene            complement(975..1505)
                     /locus_tag="PLM79_17085"
     CDS             complement(975..1505)
                     /locus_tag="PLM79_17085"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOV88073.1"
                     /translation="MSLGNIALIGFRTTGKTRVGRILARKLARTFVDMDDFLTQSFGT
                     TIDCWVRRHGWKSFREQESELLSRLAEMDGVVVATGGGVVLNRDNREILKRAFQVVWL
                     KASADTIESRMLADPVSVTQRPPLSSLPLREEIETLLRERTPLYAAAARISVDTDLLC
                     EEGVVERILDLVEREQ"
     gene            1538..2239
                     /locus_tag="PLM79_17090"
     CDS             1538..2239
                     /locus_tag="PLM79_17090"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOV88074.1"
                     /translation="MAAERLTAEAVQKEFRRPILADRFPRLPLLLGRLLRLWHRSCRF
                     TLLGTRYEEEVLSRGPAVLYACWHFAYPAVIYHFRDRNGMLMVSRSRDGEWIARVLEH
                     MGFRSARGSPGKGGGTALRRMIGHIKEGYPGGLIADGSQGPPLVAQKGILVLARHTGV
                     PLVPVSMAARPCWRFPSWDRTVLVKPFGHVALAFGPPIRVAPNASSEDLEGVRRTLQE
                     RLRELTHQCEEALSR"
     gene            2416..2794
                     /gene="rnpB"
                     /locus_tag="PLM79_17095"
     ncRNA           2416..2794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM79_17095"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2829..3554
                     /gene="ispD"
                     /locus_tag="PLM79_17100"
     CDS             2829..3554
                     /gene="ispD"
                     /locus_tag="PLM79_17100"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOV88075.1"
                     /translation="MPKTYAVIAAAGRGLRMGKKTPKQFLEIAGKPILHHTLANVFRA
                     RFLDGIFLVVPEDYLDESRKLADRVREEASRGRDPGGVPIGVIAGGPERQDSVFNALS
                     VLPREVEWVLIHDGVRPFPSARLLEETWETARRTGAAITAVPSTDTVKRVLEREVVQT
                     LAREEIWLVQTPQVFRKDLLLEAYGAARRNGWTATDDAALIERLGKPVTVVEGERSNI
                     KVTTPADVQWAAWYWSSRGNDRE"
     gene            3613..4137
                     /gene="ispF"
                     /locus_tag="PLM79_17105"
     CDS             3613..4137
                     /gene="ispF"
                     /locus_tag="PLM79_17105"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOV88076.1"
                     /translation="MRVGFGYDVHRLVPDRPLILGGSSIPHTHGLLGHSDADVLLHAI
                     CDGLLGAASLGDIGRHFPDSDPEFKGISSLILLERTMELVRQRGWELENVDCTIVCQK
                     PRLAPYIPRMVQEIARATGAPPHCINVKATTTEHLGFAGREEGIAAYATVLLRRGPAR
                     PGEEQTAREPGRGA"
     gene            4236..>5153
                     /gene="cysS"
                     /locus_tag="PLM79_17110"
     CDS             4236..>5153
                     /gene="cysS"
                     /locus_tag="PLM79_17110"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOV88077.1"
                     /translation="MALQVYNTLTKRKEEFVPLEPGKVRLYVCGVTVYDVCHIGHARS
                     AIVFDVIYRYLTHLGFDVTYVRNFTDIDDKILRKCSEENTDYRTIADRYIAGFNQDMA
                     VLGTLPPTFEPLATDHIPQMLEIIRRLVDKGFAYQAGSDVYFEVEKFPEYGKLSGRRL
                     EDMMAGARVDVDVHKRNPLDFVLWKGSKPGEPAWDSPWGPGRPGWHIECSAMGNQYLG
                     ATFDIHGGGKDLIFPHHENEIAQSEAAFGVPFVRYWIHNGFVNINNEKMSKSLGNFFT
                     IRDVLERVHPEALRLFVLSKHYRSPVDFSD"
BASE COUNT         1024 a         1555 c         1619 g          955 t
ORIGIN      
        1 ccgtgagcct ccgccagcag gtcgggtcga gccttcccca cggaattgag gacggcggcc
       61 gttaccaggc cttcgatcac cccgatggca aggtggatgg gctgcatgag caggagaaac
      121 gtgtccatgg gcagctcgga aatccccgag agggtggtct gaatcacaac ggaaagggcg
      181 cccaactgga gcccaacgat ggacgcgacg gaggccccgg ccaggacgcg gcccggggtg
      241 ggacgaccgg aaacgatctt tcggtaaagg agagggtagg ccacgaaaca ggggaagaat
      301 cccaggttta agacgttgca gcccagggcc aaaatgcctc cgtccgcaaa gaagaatgcc
      361 tggatggtca aaatagaggc catggtgaga aaggcggcgt ggggtcccag gagcacggca
      421 aggatcattc cccctcccag gtgtccgctc gatcccgtcg cgggaatgga aaagttgatc
      481 atttgtgctg caaagatgaa agctcccaga acccccatga gaggggcttt cctctcgtcc
      541 ggatctttct tcaccttcgc ggcgctgtaa agggtcagcg ccgccgaggc gccccacatt
      601 gcagcgccca cccccggaga aatcaatgcg tcggccatgt gcatggatcc aattcctttc
      661 cttgcggcac gcgggaccgg gcctcgtggg ctttggcggc ggttctggtc cgccggggtg
      721 ttcctgccgt ggaacaaccg ggtgatttgg cccttcagtg aagcccgacc cgcaaacaaa
      781 acaggccctc gggaccctcc agggatcact caggccttca tggccggaca tgggagttgc
      841 ggggagtgca aaactttggt tctcttcgga gcttcatgct cccgttgaac ctaaactacc
      901 cggtccgtca gggagtgtca atccgatttt gacgttcgat cctgccgccg acaccgggta
      961 ccggtcccgg ggtctcattg ctccctttcc acaaggtcga ggattctctc cacaacccct
     1021 tcttcgcaca ggaggtccgt atctaccgag attcgggctg cggctgcata caggggcgtt
     1081 cgttcccgaa gcagggtttc gatctcctcg cgcaggggca gggaagagag gggcgggcgc
     1141 tgggtaacgg acacggggtc cgccaacatt cgggattcga tggtgtcggc cgacgccttc
     1201 agccagacga cttggaaggc ccgtttcagg atctcccggt tgtcccggtt cagaaccact
     1261 ccaccccccg tggccaccac cacgccgtcc atttccgcca gcctcgagag aagctccgat
     1321 tcctgctctc gaaaggactt ccatccgtgt cggcgcaccc agcaatcgat ggtggtccca
     1381 aaggattggg tgagaaagtc gtccatgtcc acaaaggtcc tagccagctt ccgggcaaga
     1441 atccttccca cacgagtctt ccctgttgtt ctgaaaccga tcaatgctat attaccgagg
     1501 ctcatgaatc catgctcact cgaagtaggt cgataccatg gcggcagaaa ggctcacggc
     1561 agaggcggtg caaaaggagt tccggcggcc catcttggcg gatcgattcc cccggctccc
     1621 cctcttgctg ggacggttgc ttcgcctgtg gcatcgaagc tgtcggttca ccctcctggg
     1681 aacccggtac gaagaagagg tcctctctcg ggggcccgcc gttctctatg cctgctggca
     1741 ctttgcttat ccagccgtga tttaccattt tcgagaccgg aatggaatgc tcatggtcag
     1801 ccgcagccga gacggggagt ggattgcccg ggtgctcgaa cacatgggat tccggagcgc
     1861 ccggggttcc ccgggcaagg gaggcggcac ggcgcttcgc cggatgatcg ggcatatcaa
     1921 agagggctac ccggggggcc tcatcgccga cgggtctcaa gggcctcccc tggttgctca
     1981 aaagggaatc ctggtcctgg cccggcatac cggggtgccc ctggtgcccg tgagcatggc
     2041 ggcccgccct tgctggcgct ttcccagctg ggatcgaacg gtgctggtaa agcccttcgg
     2101 gcatgtggcc ctggctttcg gtccccccat ccgagtggcc ccgaacgcct cctccgagga
     2161 cctcgagggg gtgagaagga ccctgcagga gcgcctcaga gagctcaccc accagtgcga
     2221 ggaagccctt tcccggtgat gtctttgtcg atcctttgtc cactggaacc ttgaaaagtc
     2281 gttggggctt tgatatttgg tccgtggaat ccgcctgcgg ctcgatgcaa cgagcctttg
     2341 gtcgacgggc cgtggagggt cccgggtttt tcctgccgga gggcagggtt tcccgagtca
     2401 ttcggttggg ggtttggagt agagcaaatg atcgctggtt ccgcggaagc ggaacgggag
     2461 gaaagtccgg gctccacagg gcaggatgct gggtaacgcc cagtctcggc gacgaggagg
     2521 acagtgccac agaaagcaaa ccgccccgcc tcgtgcgggg taagggtgaa acggtgaggt
     2581 aagagctcac cagtcccgat ggcgacatcg ggagctaggc aaaccccatc cggtgcaaga
     2641 ccaaatagga gagcgtttga gggcggcccg tccgagctct cgggtaggtt gctggacctc
     2701 gtcggtaacg gcgaggctag aggaatgatc attgcccggg gaccccctcc ggggggccca
     2761 gggaacagaa cccggcttat gatctgctcc agaccccctt tctttttcac cccttgaaac
     2821 gtagccgtat gccgaaaacc tatgccgtca tcgccgccgc cgggcggggt ctccggatgg
     2881 gaaagaagac ccccaagcag tttctcgaga tcgcggggaa gcccattctg caccacactc
     2941 tggccaatgt gtttcgggca cgctttctgg acggcatttt cctcgtggtg cccgaggact
     3001 acctggacga atcccggaag ctcgcagaca gggttcggga agaggcgtcc cggggaagag
     3061 acccgggggg cgttcccatt ggagtgatcg ccggggggcc cgagcggcag gacagcgtgt
     3121 tcaacgcact gagcgtgctt ccccgggaag tggagtgggt cctgatccat gacggagtcc
     3181 gtcccttccc gtcggcccga ctgctggagg aaacctggga aacggcccgg cgcaccgggg
     3241 cggccatcac tgcggtgccc tccacggata ccgtgaaaag ggtccttgaa cgggaggtcg
     3301 tccagaccct tgcccgagaa gagatctggc tggtgcagac cccccaggtt ttccgaaagg
     3361 acctcctgct ggaagcgtac ggagccgcaa gacgaaacgg ctggaccgcc acggacgatg
     3421 cggccttgat cgaacggctg ggaaaaccgg tcacggtggt ggaaggggag cggtccaaca
     3481 tcaaggtcac caccccggcg gatgtgcaat gggccgcctg gtactggagc tcccggggca
     3541 acgaccggga atgacgggcc gggctgtccg cggagattgc ggggaccgga ctcttcgtgg
     3601 aaggaagatc acatgcgggt gggtttcgga tatgacgtgc accggttggt gcccgatcgg
     3661 cccctaatcc tgggcgggag ttccataccc cacacccacg gactcctggg gcattccgat
     3721 gccgacgtcc tgctccacgc catctgcgac gggctgttgg gggccgcgtc cctgggagac
     3781 atcggccgtc atttcccgga ttcggacccc gagttcaaag ggatttccag cctgattctc
     3841 ctggaacgaa ccatggagct ggttcggcaa agggggtggg agctcgaaaa cgtcgattgc
     3901 accatcgtct gtcaaaagcc tcgcctggcc ccttacattc cccgcatggt ccaagagatc
     3961 gcccgggcaa ccggtgcacc gccccattgc atcaatgtga aggccaccac caccgaacac
     4021 ctgggttttg ccgggcgaga agaggggatc gccgcctatg caacggtgct cctgcgaaga
     4081 ggaccggccc gcccaggaga ggagcaaacc gcacgagaac cgggtcgggg ggcatgatcc
     4141 gtccatccgg ccttgcgacc tcccccggtc ctccttcgag ggaggaggca agaaggtcgc
     4201 cgccccggac agagagggaa ggagaaccga cgagaatggc attgcaggtt tacaataccc
     4261 tcaccaagcg caaggaagaa ttcgtccccc tcgagccggg aaaggttcgg ttgtacgtgt
     4321 gcggagtcac ggtctacgac gtttgccaca tcggacatgc ccgttctgcc attgtcttcg
     4381 atgtgatcta ccggtacctg acccacctgg gctttgatgt gacctacgtg cggaatttta
     4441 cggacatcga cgacaagatc ctccgcaagt gttccgagga aaacaccgac taccgaacca
     4501 tcgcggaccg gtatattgcc ggcttcaacc aagacatggc cgtgctgggc accctgcctc
     4561 ccaccttcga acccctggct acggaccata ttccccagat gctcgaaatc atccgaaggc
     4621 tcgtggacaa gggattcgca taccaggccg gttccgacgt ctatttcgag gtcgagaaat
     4681 tccccgagta cggcaagctg tcgggtcgcc ggctggaaga catgatggcg ggggcccggg
     4741 tggacgtgga cgtccataaa cgaaaccccc tcgactttgt cctctggaaa ggcagcaaac
     4801 cgggtgagcc cgcatgggat tccccctggg gcccgggacg ccccggatgg catatcgaat
     4861 gttccgccat gggaaaccaa tacctgggag cgactttcga cattcacggg gggggaaaag
     4921 acctcatctt tccccatcat gaaaacgaga ttgcccagag cgaggccgcc tttggggttc
     4981 cgttcgtgcg ctactggatc cacaacgggt tcgtgaacat caacaacgag aagatgtcca
     5041 agtccctggg caacttcttc accattcgcg atgtgctcga gagagtgcac cccgaggccc
     5101 ttcgcctctt cgttcttagc aagcattacc gaagtccggt ggatttttcc gat
//
LOCUS       DAOOVC010000219         5050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9276_length_5050_cov_6.058659, whole genome shotgun sequence.
ACCESSION   DAOOVC010000219 DAOOVC010000000
VERSION     DAOOVC010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9276_length_5050_cov_6.058659"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PLM79_17115"
     CDS             <1..472
                     /locus_tag="PLM79_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOV88078.1"
                     /translation="HLFSLFHEDRHMAPGESSCLAETPWGPAGLAVCYDIRFPELFRT
                     YALKGAKLVFCPAAFPYPRLEHWKILVRARAIENQMYLVAVNQVGSEDIGPRGSVTYF
                     GTSMIVDPWGRTVVEAGETEEMLLTATFDPDLADEIRKNMRVLQDRRPDIYELG"
     gene            complement(563..1612)
                     /locus_tag="PLM79_17120"
     CDS             complement(563..1612)
                     /locus_tag="PLM79_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88079.1"
                     /translation="MKRCQRCVLPGTYPGIVFNEHGVCNRCLSHEPFHCLGEDALRQV
                     LESCRNRGPWDCLVPMSGGSDSTYVAYRLVRDYGMKVLGYHYDNHFIAPIARGNLARI
                     ENLLGIEIVRESSRLDTRLKRLFIRLNLHKSPLHLLFAFCHGCPDAIWGGARRIARQK
                     RIPLVISSESREEDASYKRTLWDRVKPSPREKIRWAARMPANFALRKCLGRLLEWRYP
                     RPKSWKNPEKIEYFAYRKQDRPAMLSVLTREVGWEHPSGNYPWRFDCLIHPVVEQMTF
                     NLLGMTEKDDLFSVLIREGQMDRREALTILESWEGRRKEQLPLIEGVLKRLGLDVAER
                     SRVLRYCEKGIWSGA"
     gene            complement(1658..2641)
                     /locus_tag="PLM79_17125"
     CDS             complement(1658..2641)
                     /locus_tag="PLM79_17125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOV88080.1"
                     /translation="MIQIWGFSLLALLSVFFFGIPGAHAVSSALVHGKNMHSEVWLGM
                     TKQETIDRIGVPSRIKSEGSCFHYDSLDVAVYFDSSMRAERIYLGRESRSGITRFAPG
                     SVTMDDIFETFGPPSSTWRLTYTPSPLVQNRATVESESSIKKGTVPRDLPLEYRGQRV
                     LYELAHAEGLLKYKYVLDDDGVAFWFDPSKTLYAAVLYPSATRDLPAVSSSGAQGSRT
                     AVVYFDFDKFKVKPAYLPVIREYAGEMRQQPGGVLHIEGHTDSVGSARYNRRLSERRA
                     SSVARLARDQGVSKDRIKKVGFGESRPAATNANPQGRAQNRRVEMRIEPSP"
     gene            complement(2789..3814)
                     /locus_tag="PLM79_17130"
     CDS             complement(2789..3814)
                     /locus_tag="PLM79_17130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV88081.1"
                     /translation="MLLDKRLLLLINWEEEGRWDLYRSIRDRWKRVIILQPYRVPCPG
                     YGTFNRFSTWASEMYLPFLALCKRRQADVVVSWSMRMGVCYGILNRLFPGGNRCRHVL
                     YDFHINLRKKGAGYRRKLRLLRFALGGIDFFLCTSNREADLYSHLFRVPRERIRFFPM
                     TPPRHYLDAYDFPRKDYVLSYGNSDRDYDSLVTAAKESGLPTVILTQTYRFFSPLPSN
                     VRVIRERTVGLDLIRLIASARLVVLPLRDAMVSAGQTAMLETMALGRPLIVSANPATA
                     EYAQHGRDALFFEPGDARALKERMLFLWEHPGEAERLGARARESALEFPNRQVAVFLD
                     TLENLLL"
     gene            complement(3801..4865)
                     /locus_tag="PLM79_17135"
     CDS             complement(3801..4865)
                     /locus_tag="PLM79_17135"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV88082.1"
                     /translation="MKRKMLITGGAGFIGFHSTRFFSRNGWQVAVVDNLSRKGSPYNL
                     ECLRSRATFTHYPTDVRDYPGMKEVFRKEGPFDAVLHLAAQVAVTTSVVNPRHDFEVN
                     ALGTLNLLECVREHSPSGLFLYASTNKVYGKLEDLPLVGTDSRYCFEDTEGVDENRSL
                     DFHSPYGCSKGCADQYVRDYARIYGLHTVNFRQSCIYGDQQLGVEDQGWVAWFIIAGT
                     LGLPVTVYGDGKQLRDLLWIDDLVRLYALACDNGRRLRGGIYNIGGGYANTLSLRELL
                     AFLDRRGVSLQYGHGDWRPGDQRVFVSDNRKAERELGWKPVVGVEEGLDRLIEWVREN
                     RPMLRGMLAAKMPGGSGAAR"
     gene            complement(4862..>5050)
                     /locus_tag="PLM79_17140"
     CDS             complement(4862..>5050)
                     /locus_tag="PLM79_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88083.1"
                     /translation="ASGILALLRDDARRLRMGREAQRLVEEQLSVQRMSAEFERLLLR
                     TYHRKCRSHTTNGEPIRV"
BASE COUNT          999 a         1548 c         1511 g          992 t
ORIGIN      
        1 ccacctcttc agcctgtttc acgaggatcg gcacatggcc ccgggagaat cctcctgcct
       61 ggcggagacc ccctggggtc cggcgggcct ggctgtctgt tacgatatcc ggttcccgga
      121 gctcttccgg acctatgccc tcaaaggggc caagctggtc ttctgtcccg cggcctttcc
      181 ttatccccgc ctcgaacact ggaagatcct ggtgcgggcc cgggccatcg agaaccaaat
      241 gtacctggtc gccgtcaacc aggtggggtc cgaggacata ggaccccgcg ggtccgtcac
      301 ctattttggc acctcgatga tcgtcgatcc gtggggaagg accgtggtgg aggccggaga
      361 aaccgaagag atgcttctca ccgcaacctt cgatccggat cttgcggacg agatccgcaa
      421 gaacatgcgg gtcctccagg accgtagacc ggacatttac gagctgggtt gatctgaaaa
      481 agaccggggc tgaggggttt cccttgccgg tcgagggccg ggggccgggg gccggggggc
      541 ggacgagaaa tcccggaacg gttcaggccc cgctccagat gcccttttcg cagtatctca
      601 aaacccggga ccgttccgcg acatcgagtc ccaagcgctt caggacccct tcaatcaacg
      661 ggagttgctc cttccgtcgc ccttcccatg attccaggat ggtgagggct tctcttcggt
      721 ccatctgccc ctcgcggatc aggacgctga acaggtcgtc cttttccgtc atgcctaaga
      781 ggttgaaggt catttgctcc accaccgggt gaatgagaca gtcgaacctc caagggtaat
      841 tgccggaagg gtgttcccag cccacctccc gggtgaggac cgaaagcatt gcgggccgat
      901 cctgcttccg gtaggcgaaa tactcgatct tttccgggtt tttccatgat ttcggccgag
      961 gatagcgcca ttccaacaag cgacccaggc acttgcgcag ggcaaaattc gcgggcattc
     1021 gggcggccca ccgaatcttt tctcgggggg agggtttcac acggtcccaa agggtgcgct
     1081 tgtaggatgc gtcttcctcc cggctttccg acgaaatcac caagggaatc cgcttctgcc
     1141 gcgcaatcct tcgggccccg ccccagatgg cgtcgggaca cccgtgacaa aaggcgaaaa
     1201 gcaggtgcag gggggatttg tgaagattca gccgaatgaa caggcgcttc aatcgggtgt
     1261 cgagacgact ggactcgcgg acgatttcga tccccagcag gttctcgatc cgagccaggt
     1321 ttccccgggc gatgggggct atgaaatgat tgtcgtagtg atacccgagg accttcatgc
     1381 cgtaatcgcg aaccaggcgg taagcgacat aggtggagtc gcttcctccg ctcatgggca
     1441 ccaggcaatc ccacggtccc cggtttcggc aggattccag gacctgcctg agagcgtctt
     1501 ctcccaggca atgaaacggt tcatgggaaa gacaccggtt acagactccg tgttcgttga
     1561 aaacaatccc cggataggtc cccggaagca cgcatctttg acacctcttc acaaccactc
     1621 ctgatcttgg ggaatgagct cccgctgcgt cccctctcta tggagacggc tcgattcgca
     1681 tctccacccg gcggttttgg gcgcgcccct ggggattggc attggttgcc gccggccggg
     1741 attctccgaa tccgaccttc ttgatccggt ccttggaaac tccctggtcc cgggcgagcc
     1801 tggcgacgct ggaagcccgc ctttccgaga gccgccggtt ataccgggcc gatcccacgg
     1861 aatcggtgtg cccttcgatg tgcaggactc cgcccggctg ctgtctcatc tcccctgcgt
     1921 actcccggat caccggcaga taagcgggtt tgaccttgaa cttgtcgaag tcgaagtaga
     1981 ccaccgcggt ccgcgacccc tgggcaccgc tcgaggaaac ggcgggcagg tcccgggtgg
     2041 cggaagggta gagaacggcg gcgtaaaggg tcttcgacgg gtcaaaccaa aatgcgaccc
     2101 cgtcatcgtc cagaacatac ttgtatttga gtaggccctc tgcgtgagcc agttcgtaca
     2161 agactcgctg tccgcgatat tcgaggggaa ggtcccgggg aaccgtgccc ttttttatgg
     2221 aagattcgga ctcgaccgtc gccctgtttt gtaccagggg agaaggggta taagtgagcc
     2281 tccaggtgct cgaaggggga ccgaaagttt cgaagatgtc gtccatggtg acgcttcccg
     2341 gggcaaagcg ggtgatgccg gacctggatt ctctgcccag gtaaatcctc tcggcccgca
     2401 tgctcgagtc aaagtagacg gccacatcca gcgaatcata gtgaaagcag cttccttcgc
     2461 tcttgattcg actgggaacc ccgattcgat cgatggtctc ctgcttggtc atgcccagcc
     2521 aaacctcgga gtgcatgttc ttcccgtgga ccagggccga ggaaacggcg tgggccccgg
     2581 gtatcccgaa gaagaagacc gacaagaggg ccaaaagaga aaaaccccaa atttgaatca
     2641 tggaaatcct cctattgtat gctgttggcg gcgcatgcct tcatgggttc ggcgcctggt
     2701 ccggccaatc cttgagtggg gacctgcctc accccaaccg gcttccgagc gggggcaaag
     2761 gaggccctct ccagcctgga gttgagtcct aaagcagcag gttttccaga gtatcgagga
     2821 aaaccgccac ctgccggttg gggaactcga gggcggactc ccttgcccga gcacccaacc
     2881 gctcggcctc cccgggatgc tcccacagga aaagcattcg ttccttgagg gcccttgcgt
     2941 ccccgggttc aaaaaagagg gcgtcccggc cgtgttgggc gtattccgcc gtagccgggt
     3001 tggcggaaac gatgagggga cggcccaggg ccatggtttc gagcatggcc gtctgccccg
     3061 cagacaccat ggcgtcccgc aggggaagca ccaccaacct ggccgatgca atgaggcgga
     3121 tgagatccag tcccacggtc ctctcccgga tgaccctcac gttggagggc aggggactga
     3181 agaaacgata ggtctgggtc aggattaccg tgggaagtcc gctttccttc gccgccgtca
     3241 ccagggagtc ataatcccga tcgctgtttc cgtaagaaag cacgtaatct ttccgtggaa
     3301 aatcgtaggc gtccaggtaa tgccggggcg gcgtcatggg aaagaagcgg attcgctccc
     3361 gggggacccg gaagagatgc gaatacaggt ccgcttcgcg gttggacgtg cagaggaaaa
     3421 agtcgattcc tcccagggcg aacctcagga gccgcagctt ccgccgatac cccgcgccct
     3481 tcttccgaag gttgatgtga aaatcgtaca gcacatgcct gcaacggttt cccccgggaa
     3541 acagccggtt gagaatcccg tagcagactc ccattctcat ggaccacgag acgactacgt
     3601 cggcctgtct tcgcttgcat agggcgagaa agggaagata catctccgag gcccaggtcg
     3661 aaaagcggtt gaaagtccca tagccgggac acggcacccg gtagggttga aggatgatga
     3721 cccgcttcca ccgatcccgg atggaccggt agaggtccca ccggccctct tcttcccagt
     3781 tgataagaag aaggagtcgc ttatcgagca gcaccgcttc ctccaggcat ctttgcggcg
     3841 agcatccccc ggagcatggg ccggttttcc cgcacccact cgatgagccg atcgagaccc
     3901 tcctcgacgc ccaccacggg tttccagcca agctcccgct ccgctttcct gttgtcgctg
     3961 acaaagaccc tctggtcccc tggcctccag tctccatggc cgtattgcag ggaaactccc
     4021 cggcggtcca ggaaggccag gagctcccgg agggagaggg tattggcgta tccgcccccg
     4081 atattgtaga tgcctcccct gagccggcgt ccgttgtcgc aggccagggc gtagagccgc
     4141 accaggtcgt cgatccacag gaggtcccgc aactgtttgc cgtcgccgta gacggtcacc
     4201 ggcagcccca gggtcccggc aatgatgaac cacgccaccc atccctggtc ttccaccccc
     4261 agctgctggt ccccgtagat acaggactgt cggaagttta cggtgtgtag tccgtagatc
     4321 cgcgcgtaat cccggacata ctggtccgca cagcccttgg aacagccgta gggggagtga
     4381 aaatccaggg aacggttttc gtcgactccc tcagtatcct cgaagcagta tcgactgtcc
     4441 gtaccgacca ggggaaggtc ctcgagcttt ccgtacactt tgttggtgga tgcgtagagg
     4501 aacaacccgg aagggctgtg ctccctcacg cactcgagaa ggttcagggt gcccaaagcg
     4561 ttcacctcga agtcgtgtcg cggattgacc accgaagtgg tcacggccac ctgggccgcc
     4621 aggtgaagga cggcatcgaa gggcccctct ttccgaaaga cctctttcat gcccgggtaa
     4681 tcccgaacgt ccgtcgggta atgggtgaag gttgcacggc ttcgcaggca ctcgaggttg
     4741 taggggcttc ccttgcggga caagttgtcc accaccgcca cctgccatcc gttccgggaa
     4801 aagaagcggg tggaatgaaa cccgatgaac ccggccccgc ccgtgatcaa catttttctt
     4861 ttcatacgcg tattggctcc ccgttggttg tgtgggatcg gcacttgcga tgataggtcc
     4921 gtaagaggag ccgctcgaac tcggcgctca tccgctgaac gctgagctgc tcctccacca
     4981 ggcgttgggc ttcccgtccc atccgaagcc tccgggcatc gtctcgcagc agggcaagaa
     5041 tcccggatgc
//
LOCUS       DAOOVC010000220         5007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9383_length_5007_cov_3.997375, whole genome shotgun sequence.
ACCESSION   DAOOVC010000220 DAOOVC010000000
VERSION     DAOOVC010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9383_length_5007_cov_3.997375"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLM79_17145"
     CDS             complement(<1..985)
                     /locus_tag="PLM79_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88084.1"
                     /translation="MTVLLALIGASLAIMILVGFLRRNPAGHDEESVRFATRVYAFWL
                     IASAVWGPYFYVLRIPGLFDISLDRLVLMAILAIMGLGAFTGRLFRHRQMRLELLMAL
                     FSLLCLLSMAINGFRPVNPSFPSPWNVFITGYLFPFIAFVFAKNYLTEEADIRFVLQA
                     LFYLGVYLAIISFFEFFGLRKYVYPQYINNPEIWLHLDRARGPFLNSAFNGIALIVGL
                     ACGIHLASEKEGFGRILHLALLALFFPAVFFTLTRSIYLGFLIVMTLFLFFYRTSFPK
                     WRVLALPLAVVMVFLLTQTPKLASKERREGGVLQVEEVAIRMALLQRSFLIF"
     gene            complement(998..2422)
                     /locus_tag="PLM79_17150"
     CDS             complement(998..2422)
                     /locus_tag="PLM79_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88085.1"
                     /translation="MNDVSKRKACQENQHYVKQMAFIFFDRYKLIAGIFTTVFVLTLV
                     WVLSLPPVYRSSAKFSVVIPQALDPLQRETYYDYKNRVIRYLQEQKELILSNTVLTTA
                     VQLMFPELNPELLPKMVQSFREHVEVTPPKGETFEASSVFYITATGSNPHRVYRRASA
                     ITEAYMRGYGETTKAKTDYSYDFFKEQTQTLYNEMLEKEKALREYETKQAVSLIEMLN
                     LDPTKTNLEVGPNALLTQFSRKSHELQEELAGLKTAIAKLEAEAKDDRIPVLLPEMEV
                     TGRAITAFKNRVAQLQIQLNEMRPQFSDQFAPMKQVEKELDLNVRSLRDELNRSVMAQ
                     KITAQSIEAKIQELEKIIAALQERIRSTAEERSRYEHLKQQYLLAKDTYTHSRNQLEQ
                     ARLANALNQEKQFITLVEKPEEPLEPFKPNRPVLILLGFLAGIFVSMATALTVDYFDH
                     TLKTPEDIDCHLCAEVLGSVPRIG"
     gene            complement(2474..3568)
                     /locus_tag="PLM79_17155"
     CDS             complement(2474..3568)
                     /locus_tag="PLM79_17155"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOV88086.1"
                     /translation="MTVLLVPFLVSCSSTSQKQNSEIDLHVQKPEDVTRLKNTETALT
                     VTPEKAALHPEYQTIERIAVPYIPEYRIGPSDVVEIVYHIRYDRTNEEYRLEIQDKIS
                     LHFPYHPQFSTTVLVRTDGRITVPLIGEVHAASKTPRELAALLNREYSKYINNPSITV
                     ALEEFNVKIEELKRAITTAPRGQSKIAPVTPDGRISFPIIGSLHAEGLTVDQLEKIVN
                     DKYETQVRNLRVTLILLEIHHPKFYVVGEVSRPGAYEMTSRISLLDALALANGHKKSA
                     RLSDVVVFRNDGLERPIAFKVDLEAALKQGATHVNLPVKPADIIYVPKTRLDEFNDLV
                     EKVFTRGLYAIAPFQSNFTVNYDIRNPSIR"
     gene            complement(4637..>5007)
                     /locus_tag="PLM79_17160"
     CDS             complement(4637..>5007)
                     /locus_tag="PLM79_17160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV88087.1"
                     /translation="NSSEDRDLPEIPPLEGRAAARFEHGTGVPWWIEFGGRFTAPQKD
                     VDDWFPEDETAGYAVFHLYAGCRPVKNLRIQVGIDNLFNKEYHEHLTREAVFAGGDLS
                     AGDEVPAPGISFFAVGRYDF"
BASE COUNT         1036 a         1406 c         1497 g         1068 t
ORIGIN      
        1 caaaaatcaa gaaagatcgt tggaggaggg ccatccggat ggccacttcc tcgacctgca
       61 gcacccctcc ctccctgcgt tccttggacg ccagcttggg ggtctgggtc agaaggaaaa
      121 ccatgacgac ggccaggggg agagccagga ccctccactt gggaaaggaa gttcggtaaa
      181 agaacaggaa aagtgtcatc acgatcagga aaccgaggta tatggacctc gtgagggtaa
      241 agaaaaccgc cggaaagaaa agggccagca aagccaggtg taagatcctt ccgaatccct
      301 ccttttcaga tgccaggtgg atgccgcacg caagccccac gatcaatgcg atgccgttga
      361 aagccgaatt gagaaacggc ccccgggccc tgtccaggtg aagccaaatc tccggattgt
      421 tgatgtactg tggatatacg tacttgcgca ggccgaagaa ctcgaagaac gaaatgatcg
      481 ccagatacac cccaagatag aaaagcgctt gcaggacaaa ccggatgtcc gcttcctcgg
      541 tgaggtaatt cttggcaaag acgaaagcga tgaaggggaa aaggtagccg gtgatgaaca
      601 cgttccaggg ggaggggaaa gaaggattga cgggcctgaa tccgttgatt gccatggata
      661 gaaggcatag gagggaaaag agggccatga gcagttcgag ccgcatctgc cgatgtcgga
      721 agaggcgacc ggtgaaggca cccagtccca tgatggccag gatcgccata aggaccagcc
      781 gatccagaga gatgtcaaac aatcccggga tgcgcagcac atagaagtag ggcccccaga
      841 cggcggaggc aatcagccag aaagcataaa cccgggtggc gaaccggacg gactcctcat
      901 catgccctgc gggattcctc ctcaagaatc cgacgagtat catgatcgcc agggacgctc
      961 cgatgagtgc cagcagaacc gtcatcctat atcgtgccta tcctatgcgc gggacggaac
     1021 cgaggacttc ggcgcacagg tgacagtcga tatcctcggg ggtcttgagg gtgtgatcga
     1081 agtagtccac ggtcaaggca gtggccatgg agacgaaaat tccggccagg aagcccagga
     1141 ggatcaggac cggacggttg ggcttgaagg gctcgagagg ttcctccggc ttctccacca
     1201 gggtgatgaa ttgcttttcc tggttcaatg cgttggccag gcgcgcctgt tccagctggt
     1261 tcctcgagtg ggtgtaggtg tccttggcca ggagatattg ctgcttgaga tgctcgtacc
     1321 gcgagcgctc ttccgctgtg gatcggattc gctcctggag ggcggcaatg atcttttcca
     1381 gctcctggat cttggcctcg atactctggg ccgtgatctt ctgagccatg acggatcgat
     1441 tcagttcgtc gcgcagggag cggacattga ggtccagctc tttttccacc tgtttcatgg
     1501 gggcaaactg gtcggaaaac tgagggcgca tctcgtttag ctggatctgg agctgggcca
     1561 cgcggttctt aaaggccgtg atggcccgtc ccgtgacctc catttcgggc agcaggacgg
     1621 ggatcctatc gtctttcgcc tcggcttcga gcttggcaat ggcggtcttc agccccgcca
     1681 gttcctcctg aagctcgtgg ctcttgcggc tgaactgggt gagcagggca ttgggaccta
     1741 cctccaggtt ggtcttggtg ggatccaggt tgagcatctc gatgagggac accgcctgtt
     1801 tcgtttcata ttccctcaga gccttctcct tctccagcat ttcgttgtag agggtttgag
     1861 tctgttcttt gaagaagtcg taagagtagt ccgtcttggc cttggtcgtc tccccgtacc
     1921 ctctcatgta ggcttcagtg atggccgaag ccctacgata gacccgatgg gggttggaac
     1981 ctgtcgccgt aatgtagaag acgctggaag cttcaaaggt ctcccctttt gggggcgtca
     2041 cttccacatg ctctcgaaaa ctctgaacca tcttgggcag gagctcggga ttgagctccg
     2101 gaaacatcaa ctggacggcc gtggtgagca ccgtattgga aagaatcagc tccttttgct
     2161 cctgcaggta acgaatcacc cggttcttgt aatcgtagta ggtctcccgc tgcaacgggt
     2221 ccagggcctg gggaatgaca acggaaaact tggccgagga tcgatacacg ggaggcaggg
     2281 acaacaccca gacgagagtg aggacgaaaa cggtggtgaa gatgcctgcg atgagcttat
     2341 atcggtcgaa aaagatgaag gccatctgtt tcacgtaatg ttggttttct tgacaagctt
     2401 ttctctttga cacgtcgttc atggatgcct cccggatgtc ctgagttccc tttccatcct
     2461 acgtcaggga aggttaccgg atactggggt tcctgatgtc gtagttcacc gtaaaattgg
     2521 attggaaagg agcaatcgca tagaggcccc gggtgaacac cttttccaca aggtcgttga
     2581 actcatccag cctggtcttg ggaacgtaga tgatgtctgc gggcttgacc gggagattca
     2641 catgggtggc cccctgcttg agggccgcct ccaggtccac cttgaaggca atgggtcttt
     2701 cgaggccgtc gttgcggaag accaccacat cgctcaggcg cgcacttttc ttgtgaccgt
     2761 tggccagggc cagggcatcc agcaggctga tcctcgaagt catttcatac gccccaggcc
     2821 tcgacacctc tcccaccacg tagaacttcg gatggtgaat ctcgagcagg attagggtca
     2881 cacgaagatt tcgaacctga gtttcgtact tgtcgttgac gattttctcg agctgatcca
     2941 cggtgaggcc ttctgcgtgg aggctgccga tgatgggaaa ggaaatcctt ccgtcgggag
     3001 tgaccggggc gattttgctc tgcccccggg gcgccgtggt gatggccctc ttgagctcct
     3061 cgattttcac gttgaactcc tcgagggcca ccgtgatgga gggattgttg atgtatttcg
     3121 agtactcccg gttcagcagg gcggccagtt cccggggggt cttgctcgcg gcgtgaacct
     3181 ccccgatgag aggcacggta atcctgccgt ccgttcgcac caggaccgtc gtgctgaact
     3241 gcggatggta aggaaagtgc aagctgatct tgtcctgaat ctccagcctg tactcttcgt
     3301 tcgtccggtc gtagcggatg tggtacacga tctcgaccac gtccgaaggg ccgatccgat
     3361 attccggaat gtagggaacc gcaatgcgct cgatggtctg atattccgga tgcagggcgg
     3421 ccttttccgg ggtgaccgtc agggccgttt ccgtgttctt cagccgggtc acgtcctccg
     3481 gcttctgaac gtggagatcg atctccgaat tctgcttctg ggaggtgctc gaacaggaaa
     3541 ccaggaaagg aaccagaagt accgtcacga ggagggccaa agccttccca accgtttggg
     3601 ccgtccggca acatggatga cccttctccg tcggtttcca gcataccctc ggcccgcttt
     3661 tgcagccgtc agcggttctt tccataccac tcctttaaat gggttgtccg cgttgtgttg
     3721 gagctccccg gtacggtcgc ggcaaaaagg ttaagagccg acaatccacc aatggttccg
     3781 agatcgagaa gaggaatgaa ggtccttgga tgcacgccca agggcaactg caataccccg
     3841 gcaaaggggc ttatgcagct ttcgagccgg ctccatcttt cttcgacccg acttcccggc
     3901 cccatgacgc tgttgccgac ttcccaggac ctttgaaatc ccgaaggggt ggaccggacc
     3961 cccggcgtgt ttgccgtcca cgccaagagc cggctgccgt agggccctcc aagtcccggc
     4021 gccctcccgg ccggggatcc cgggttcgcc ggcccaacct tggatcaccg gcgggtccct
     4081 ccgaaccacc cgaatttcgg aatggcgccg gtgaaggccc cggagaggca ccagccgctc
     4141 caacgggtcc attcgccgtt gctgatccac ggtggagggg gcgggcttgt gggccttacc
     4201 cttcccgcct cagtgatctc aatgtcttat cagtgagtaa actggaattc cgaatccgga
     4261 tatcgagaat atcctcacaa caacgagtgg aaaatacaga gactgtgggt tggttgtcaa
     4321 atggaatgtc acaaaggaag cggtaattga ccctgttttg tacgtttttt cggaccaata
     4381 ggtgaatgac tccccccacg ggacttgcct acggaaaaat ccacgggcgt tcgtggggtg
     4441 ccgggtcccc cttatgggcg tcggtgctgg cacgccggac ggcatccccc atcgcagcgg
     4501 caggtgttgc gctcatgccg ttatatcctc cgaccctcct ctccgtcggc cgctcccggg
     4561 ccggccttcg tctcgcgcgc ctctgcgccc gaacggtccc cgtcggggga tccgggcggc
     4621 aagcccggat cgacctttag aaatcataac ggcctacggc aaagaagctg atgccggggg
     4681 ccgggacttc gtctcccgcc gacaggtctc cacccgcaaa caccgcctcc cgggtgagat
     4741 gctcgtggta ttccttgttg aacaggttgt cgatccccac ctggatacga aggttcttga
     4801 cgggcctgca ccccgcgtaa aggtggaaga ccgcatagcc cgcggtttca tcctcgggga
     4861 accaatcgtc cacatccttc tggggggccg tgaaccgacc cccaaactcg atccaccagg
     4921 ggactccggt gccgtgttcg aaccgggccg cagcccgccc ctcgagggga gggatctcgg
     4981 gaagatcccg gtcctcggag ctgtttc
//
LOCUS       DAOOVC010000221         5003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9392_length_5003_cov_6.291229, whole genome shotgun sequence.
ACCESSION   DAOOVC010000221 DAOOVC010000000
VERSION     DAOOVC010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9392_length_5003_cov_6.291229"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1179..1886
                     /locus_tag="PLM79_17165"
     CDS             1179..1886
                     /locus_tag="PLM79_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOV88088.1"
                     /translation="MSLGERLESLRGNRSLGDFAKLFGIHKNTYANYIKQKSVPDSGF
                     ILAVCQKFDLSANWLLMGIGPKFLDQRIHQEMPQTSRGGKGQVLVPLIETFVIDREGD
                     IIHDGIIDHLPLPWDWIDGGDGEGEEDLTKSFLFLRVRGDSMSPTVHRGDIVLVDTRR
                     EKRTFVQTGSIFYVRLPDGERAFKRVAVFQKEGEHKVLFYSDNVTAYPPVEFTVQAMD
                     HLHCHLLGLVRWSYRRF"
     gene            complement(1984..2442)
                     /locus_tag="PLM79_17170"
     CDS             complement(1984..2442)
                     /locus_tag="PLM79_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88089.1"
                     /translation="MPALVSVLVVLCAPCEATVLQIWPDQLKPTGYSTSYYQLPSELR
                     ITDTTKEVDFFAPVSIPVGRTITKMHYYRSAFAANPTTVVLYRVKMGQKPSLVALGYS
                     VAVTNRDIVLVSCALGNAVVVEKDYRYFVRVFIDNQDSDTAVQGIKIVYQ"
     gene            complement(2779..3195)
                     /locus_tag="PLM79_17175"
     CDS             complement(2779..3195)
                     /locus_tag="PLM79_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88090.1"
                     /translation="MRMGRMVLSLGLAGFVAVFLGGVAQAACVQSDIAGTWYIHLTNE
                     DEDGAWRLWAYCTVKVKSTGAVVAGTACKTSQGDTQTINGGKFTVGSDCVWNGNLKIG
                     NGAVIDHGIVNRGKDFVSAVGHDQNGGLIKLDGVKK"
     gene            complement(3403..4338)
                     /locus_tag="PLM79_17180"
     CDS             complement(3403..4338)
                     /locus_tag="PLM79_17180"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HOV88091.1"
                     /translation="MTARRSWILLVLALVFFPLETRPGIDNGRFFLMGDGNLRIHNTH
                     TEKEVGVELLAPDGSFREEGFRKIDEVFGFPTEEKGEHISPRLIFMLDYFCDLAGPDK
                     TVHLVSGYRSPEYNTSLRNAGANAAKTSQHLDGMALDFYLDGIDGKELWELIRRKNCC
                     GVGHYGGKTIHLDSARPRFWEASTSKVRTGESEQNRRIYVSSDFDRYRPGDPVRLSFS
                     SVSDFGFGVGPTVFLVDDPEGKHAVAEARLHGGEGSDCLMIRDRRSSRFLHLTLPSNL
                     HQGRYRLRVDFCSKPFEAMPERVVSNEIEVLKAAP"
     gene            complement(4370..>5003)
                     /locus_tag="PLM79_17185"
     CDS             complement(4370..>5003)
                     /locus_tag="PLM79_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88092.1"
                     /translation="GKRKWKSCSNGVRRFLSRMGKSGEAFAPTLKEAAAMKKSLMRTV
                     PAIALPAAGPVLARTYTFPAKKPILSVDLPDTWRVRLDTEDADVFAQSPHRDIEYILR
                     ELERTAVKANVTDLLNDAVKEADAVSAESVSDPQFTDWKPETVNGMQFLWATGTGKDK
                     ETGGKVNMEVDFFSTDGKREFVLPYYGMPDGERRYEQEIDKIDRSIKRIQ"
BASE COUNT          998 a         1518 c         1446 g         1041 t
ORIGIN      
        1 aggttcccgc cggggagttg aaagacggat gaggccgctg ccacaagaca cccttgagat
       61 cgaaaaggct caccaaaaac gtgaatcaat tcctggatac catcaaatcc tcgaggggta
      121 aaggacgttg acgaccatcg tttcgcggca aaatcacggc tccggcggaa agcaaacgac
      181 ccggcgggcc tcgggtaccc gggcccgccg gatcttgtct cgggacgcag cttgcaacga
      241 aaggcggttc gaggcaagcc gccccgagat catcacttgt cgtcgtcccc ctctttgggc
      301 ttcttcccct cgaaggcgaa ggagaccagg tatcctacga ttccacagag aagaatgact
      361 acgatgatgg tattgaacag tcccatgatt cgtacctcct gaatttcaga atccgcgttt
      421 gtgggcgcag gggggacccc aacggggccc cggacgaatt ctctaccggg caacaggcgc
      481 ctccgggcac gaccgaaaga aatcacctcc tcctcacgtc ccgggacacg ggacttcttt
      541 tcccgttacc cctttcccga gtgcacggtt tggtcgtgca attcccacga ccccatgaac
      601 attttattcc attgcacact atcctgtgtt gtataatcta ttatcaagta cttctatccg
      661 cttcatggac agtatcctac actaaactat tctcgtcaac gtacattatg atgcttttat
      721 ccgctttcat ggatatgttc ggggaagcag cttcaccggg aacagaggag gcgggcactt
      781 tgggagggcc gaaggcacgg ttccggcgta gggcggcacc gtctcaaaca ggggtcatga
      841 atcccctttg cgtgcggatg aatttccgtt cccggtttct caccttcccc cttgacacga
      901 tttccggttc catgtaccaa ttttcttatt aatccaaact tttgtttcca aggatcgagg
      961 gctgccgccc ctcggggcgg gcatcgtccg gcaaggagaa atggaggacg gggggccttt
     1021 ggggcctccc ttctccgaaa tgggggccgt gggacccaag atcggggttt tccggcggga
     1081 agaacggcgg aaatcggagg atgcgggagc cggttcggtt tgccgaccaa ggcctttccc
     1141 gcaaacggct aatgacgatg ggaggggtgg agcggagcat gtccctcgga gagcgtctgg
     1201 agagtctcag aggaaaccga tctctgggcg attttgccaa gttgttcgga attcacaaga
     1261 atacctacgc caattacatc aagcagaaga gcgtccccga cagcggtttt atcctggccg
     1321 tctgccagaa gttcgatctc agtgccaatt ggctgctcat ggggatcggg cccaaatttt
     1381 tggaccagag gatccaccag gaaatgcctc agacctcccg gggagggaaa ggacaggtcc
     1441 tggtccccct catcgaaacc ttcgtcatcg atcgggaagg ggacatcatc cacgacggga
     1501 tcatcgatca tctccccttg ccctgggact ggatcgacgg cggggatggc gaaggcgaag
     1561 aagacctgac gaagtccttt ctctttcttc gagttcgcgg ggattccatg tcccctaccg
     1621 tccaccgggg agacatcgtc ctggtggata ctcgccggga aaagcgcacc ttcgtgcaaa
     1681 cggggtcgat cttctacgtg aggctgcccg acggggagag ggctttcaag cgggttgcgg
     1741 tcttccaaaa ggaaggagag cacaaggtcc ttttctactc ggacaatgtc actgcctacc
     1801 cgcccgtcga gttcaccgtc caggccatgg accatctcca ctgccacctt ttggggttgg
     1861 tgcgctggtc ttaccggcga ttctagaggg ccgagtcgcg ttccaggcca agtccgcatc
     1921 cctccccaca caccaatggg atcaaggggg acaggggcag acggatgagg tttgggcggg
     1981 gagttactgg tacacgatct tgatcccctg gacggcggtg tccgaatcct ggttgtcgat
     2041 gaagactcgg acgaaatagc ggtagtcttt ttcaacgacc acggcgttcc ccagggcgca
     2101 ggaaaccagg acgatgtctc tgttggtcac ggcaacggag tatcccaggg ccacgaggga
     2161 gggtttctgc cccatcttca cccgatagag aacgacggtg gtggggttgg cggcgaaagc
     2221 actgcggtag tagtgcattt tcgtgatggt gcgtcccacg gggatcgaca cgggggcaaa
     2281 gaaatccacc tccttggtgg tgtcggtgat cctcagctcc gagggcaact ggtagtagct
     2341 ggtgctgtag cccgtgggtt tcagttgatc gggccagatt tggaggacgg ttgcctcgca
     2401 cggggcgcaa agaacgacaa gcaccgaaac caaggccggc aaacatcccg cacatctcct
     2461 cctccacttc ccatgcttct tcatggcgtt cctcccatgt cgccgatggg gcggatcccc
     2521 catcggtcgg ccccgtcaaa ccggggctgc ctcctcaccc ctttgggtga ccgttccaaa
     2581 acacgccgta gattccgctt tcccatggga cccttcccca ggcgttcgta cactcgaaag
     2641 gccgccgaga gcgaccgagg tccggtgtcc ggggtgggag cgggagtcac tccggcactt
     2701 ggtcgagcaa cggcatagtc ctccacgcgc catgccccgc cgtgactccc gaaagctcca
     2761 ctcccggttg cggggaaact acttcttcac cccgtccagc ttgatcaggc ccccgttttg
     2821 gtcgtgcccc accgcgctga cgaaatcctt gccccgattg acgatcccat gatcgatcac
     2881 ggcaccgttg ccgatcttga ggtttccgtt ccagacgcaa tcggacccca cggtgaattt
     2941 gccgccgttg atcgtctggg tgtccccctg ggaggtcttg caggcagtgc ccgccaccac
     3001 ggcgccggtg gacttgacct tgaccgtgca gtaggcccaa agccgccagg caccgtcttc
     3061 gtcctcattg gtcagatgaa tgtaccaggt cccggcaatg tcgctctgaa cacaggccgc
     3121 ttgtgcgact ccccccaaga ataccgcaac gaaaccggcc aaccccaacg aaagaaccat
     3181 cctgcccatc ctcatgattg tcctcctctt gtgaattccc cgcgaaagcg gtgggttgtt
     3241 tggcacgaac cccctgcaac gagcccaatt ccccggaagc gggtgtacag ccctgtaccc
     3301 ggggaaattc gtccttggag tggaccccgg tcccgggccg ggcaaaccgc attttctccc
     3361 ttcctttgac ggacggggcc gcacacggga accccggaaa cctcagggag cggccttgag
     3421 aacctcgatc tcgttggaca cgaccctctc cggcattgcc tcgaagggct tgctgcaaaa
     3481 gtcgacccgc aaccggtatc gtccttggtg cagattggac ggcagggtca ggtggagaaa
     3541 acgcgaggag cgccgatccc ggatcatgag gcaatccgaa ccctcccccc cgtgcagccg
     3601 ggcctcggcc acagcgtgtt ttccctcggg atcgtccacc aggaacaccg tcggtccgac
     3661 cccgaagcca aagtcgctca ccgaagaaaa ggaaagccgg accggatctc ccggcctata
     3721 gcggtcgaaa tccgaagaca catagattcg ccggttctgc tcactctctc ccgttcgcac
     3781 cttggaggtc gaagcctccc agaaccgggg acgagccgaa tccaggtgga tggttttccc
     3841 cccgtaatgg ccgactccgc aacagttctt cctgcggatg agctcccata gttccttgcc
     3901 gtcgatgccg tccaggtaaa agtccagggc catgccgtcc aggtgctggc tggtcttggc
     3961 ggcattggcc cccgcgttcc gcagggaggt gttgtattcg gggctccgat agccggacac
     4021 caggtggacg gtcttatcgg gtccggcaag atcgcaaaag taatccagca tgaagatcag
     4081 gcgcggcgag atgtgttccc ctttctcttc cgtaggaaac ccgaacacct cgtcgatctt
     4141 tctaaatccc tcttcccgaa aggacccgtc gggggcgagg agttcgaccc cgacttcctt
     4201 ttccgtatgg gtgttgtgaa ttcgaaggtt gccgtccccc atgaggaaga aacggccgtt
     4261 atcgataccg ggccgggtct cgagagggaa aaagaccaaa gccaagacga gaagaatcca
     4321 tgagcgccgt gccgtcaccg tggtcaggtc ctccgtaagg tttgctgatc tattggatcc
     4381 tcttgatgga acggtcgatc ttgtcgatct cctgctcgta ccgccgctcc ccgtcgggca
     4441 tgccgtaata cgggaggaca aactcccgct tgccgtccgt ggaaaagaaa tcgacctcca
     4501 tgttgacctt gcccccggtc tccttgtcct tgccggttcc cgtagcccag aggaattgca
     4561 tgccgttgac ggtttcgggt ttccaatcgg tgaattgggg gtccgagacg gattccgcgc
     4621 tgacggcgtc ggcctccttg accgcatcgt tcagcaggtc ggtcacgttt gccttgacgg
     4681 ccgtcctttc cagctcccgg aggatgtact cgatatcccg atggggagac tgggcgaaca
     4741 cgtcggcgtc ttcggtatcc agccggactc tccaggtatc cggcaggtca acggatagga
     4801 tgggcttttt cgccggaaag gtgtaggtcc tggcgagaac gggtccggcc gccggcaatg
     4861 cgatcgccgg gaccgtccgc atcagggatt tcttcatggc cgctgcctcc ttgagagtgg
     4921 gtgcaaacgc ttcgccgctt ttccccatcc gggaaaggaa ccgccgtaca ccgttggagc
     4981 aggacttcca ttttcttttg ccc
//
LOCUS       DAOOVC010000222         4957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9503_length_4957_cov_5.413301, whole genome shotgun sequence.
ACCESSION   DAOOVC010000222 DAOOVC010000000
VERSION     DAOOVC010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9503_length_4957_cov_5.413301"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLM79_17190"
     CDS             <1..202
                     /locus_tag="PLM79_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88093.1"
                     /translation="AKRWSPTSFTPRGFDDIQELIQVFGELFRSRGCLVAAASSVFQL
                     PGGNLWKVNEYGDEQCGIPGNP"
     gene            165..1280
                     /locus_tag="PLM79_17195"
     CDS             165..1280
                     /locus_tag="PLM79_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOV88094.1"
                     /translation="MATNNAVFLEILEWFDQTGKELVHRIPEGGSADIKLGAQLIVRE
                     SQAAVFFYNGKAYDAFGAGRHTLTTANIPVLTKALSLPWGFTSPLRAEVYFVNMKVFA
                     NLKWGTRDPVAFKDSQLGLIRLRAFGVFNLQVVQPVLFVNSLVGTQGIFSTEEVEEYL
                     NRVIVSRFNDHMGEHLDSILSLPGRYEELSRGLMERLREDFLRFGIALPQLYINSITP
                     PPEVQQAMDDQSRLAVLNGLDGLVKMKAAMAMEKASESRGEAGTGVGLGLGMMVPGIF
                     ADALKGKREAETGPIQCPDCRQAVEADARFCPRCGHQLVVFDRCSHCGKNLTPHAKFC
                     SRCGHSVSEKPPPRKCGRCGAENLSDSVYCNRCGERI"
     gene            1267..2523
                     /locus_tag="PLM79_17200"
     CDS             1267..2523
                     /locus_tag="PLM79_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88095.1"
                     /translation="MKESDVHSWLVEWRCPQCGAPVVLEETDRILSCGYCRVRLHLSW
                     RGHWRYWLSPGKDLPADPLFVPYWRFRGIVFSCVDREIRHRVADSSHLALGLEGLPAT
                     LGFRPQALKLRPVTPTVGASFLRPETSFRSPSTVAGDAEKRSAAGDGKPPAPYEVFIG
                     ETVSVLYSPVTIRGEKVLDGILGRPLPSVVPSSLESLPFDRFPDELVTFSPMLCPRCG
                     WDLEGEKDSVVLLCRNCRSAWASRGSGLERVDFSVMVEPKEAPLYLPFWQIRARFSGI
                     RLDSYADLVRLANLPKAVQPEWEDRKARLWIPAFKIAPHLFLRLGRALTLFQGEGDPE
                     APLPSSGLHPVSLPPEEAAESLVITVAAFGVPKKTLLPKLPEIRAPMEGYSLCYVPFR
                     VTSAECIQPKIGLSLNRNALDLGRKI"
     gene            complement(2546..3667)
                     /locus_tag="PLM79_17205"
     CDS             complement(2546..3667)
                     /locus_tag="PLM79_17205"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HOV88096.1"
                     /translation="MEGQPASILALVRNLGTSSASDVTVSFFDDAPQADGTFLGSVTK
                     AALEPGASTLAEIPWNTFGQSGRNYLHILVDPEDLIEESNEENNSTILSRHIVPPTLP
                     DLAVSSTDILFSLSDPEEGDPLRVGVTVHNLLNDDTHETLKTLVEDCLTHVHREREGV
                     GFLPNELIPVKIELMSSGTSRELLIEESYPQEIRLWDPAPGEWVAQNPWGIDLHLDGD
                     EEKTLLFYARTPDQEGSYCLETRIFPGPGGGPPDQTLQLTLDVLGDKARLARDILEML
                     GSLSVSRMDLCRLQRAVFHVRHMELREVRDRRDAERAIRDALDAAESLSSITSSDVTE
                     VREKLAGLLRSWEAQWYFMGCGGTRGKTVGLDPTPAGRE"
     gene            complement(3582..>4957)
                     /locus_tag="PLM79_17210"
     CDS             complement(3582..>4957)
                     /locus_tag="PLM79_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HOV88097.1"
                     /translation="RRLAQLPPPIRMCDALSGTGGYLPCPLSIVADLDLDGIPEVIGG
                     NTVYRNDGSILWMKTKLSNGFNAVANLDEDPFPEIVPVTNGAVHVLDHTGDLKWGPVP
                     IPGGGFGGPPAVADMDGDGAPEIGTAGACRYVVYKADGTILWQSVTQDTSSDSTGSTV
                     FDFQGDGQNEVVYGDEHTLRIYRGTDGAILFQVPNSNGTAQEYPIVADVDGDRRAELV
                     VTANNYAWGSQTGIRVFEDAGNSWAKTRPIWNQHAYHITNVGDDGTIPVREANSWEQY
                     NTYRCNTLLPHETLGSPDITASLLSVDRTAYPARVAVFARIGNGGGRSPGFRGGSRLP
                     QRRPAPRGGPAGIDPHHQDPGPGGIRGGFRPMERPGRRDPRFPGRRGPPGKGPRMSTG
                     QQHGSRLVLPGRERSPVHHASRPGRLCRRRRSCTCRAHRRSTGLDPGSGSQPGDLERL
                     RRDGLLL"
BASE COUNT          857 a         1540 c         1570 g          990 t
ORIGIN      
        1 cgcgaaacga tggtcgccaa cgtcctttac ccctcgagga tttgatgata tccaggaatt
       61 gattcaagtt tttggcgagc tttttcgatc tcgagggtgt cttgtggcag cggcctcatc
      121 cgtctttcaa ctccccggcg ggaacctttg gaaggtcaac gaatatggcg acgaacaatg
      181 cggtattcct ggaaatcctt gaatggttcg atcaaacggg caaagagttg gtgcaccgga
      241 tccccgaggg agggtccgcg gacatcaagc tgggcgccca gttgattgtg cgggagagcc
      301 aggcggcggt cttcttttac aacggcaagg cctacgatgc cttcggggcg ggccgtcaca
      361 ccctgaccac cgccaacatc cccgtcctca ccaaggccct gagccttcca tggggcttca
      421 ccagccccct ccgggcggaa gtctatttcg tgaacatgaa ggttttcgcc aacctcaagt
      481 gggggacccg agaccccgtg gcgttcaagg actcgcaact gggactcatc cgactgaggg
      541 ccttcggggt tttcaacctg caggtggtgc agccggtcct tttcgtcaac agcctggtgg
      601 gaacccaggg gatcttttcc accgaggaag tcgaggaata cctcaaccgg gtcatcgtct
      661 cgcgtttcaa cgaccacatg ggagaacacc tggattccat cctgtctctt cccggccgct
      721 acgaggagct ctcccggggg ctgatggagc gtctccggga ggattttctc cggttcggca
      781 tcgccctccc ccagctgtac atcaactcca tcacccctcc gccggaagtc cagcaggcca
      841 tggacgacca gagcaggctg gccgtgttga acggcctgga cgggctggtg aagatgaagg
      901 ccgccatggc catggagaag gcatccgaat cccggggcga agccgggacg ggtgtgggcc
      961 tggggctggg catgatggtg ccgggaattt ttgccgacgc actcaagggg aaacgggaag
     1021 cggaaaccgg gccgatccag tgtccggact gtcggcaggc cgttgaagcg gatgcccggt
     1081 tctgtccccg ctgcgggcat caactggtgg tgttcgaccg ctgttcccat tgcggtaaga
     1141 atttgacccc ccacgcgaag ttttgttccc ggtgcggcca ttccgtgagc gaaaagccgc
     1201 cgccgcgaaa atgcgggcga tgcggggctg aaaatctatc cgattccgtc tattgcaacc
     1261 ggtgcggtga aagaatctga cgtccattca tggctggtgg aatggaggtg cccccagtgc
     1321 ggcgccccgg tggtgctcga ggaaacggac cgaatcctct cgtgcgggta ctgcagggtg
     1381 cgtcttcacc tttcctggag ggggcactgg aggtattggc tttccccggg gaaagacctg
     1441 cccgcggacc ctcttttcgt gccgtactgg aggttccggg ggatcgtttt ttcctgcgtg
     1501 gaccgggaaa tccggcaccg ggtggccgat tcctcccact tggctttggg cctcgagggg
     1561 ttgcccgcca ccctgggatt tcgaccccag gccctgaaac tgaggcccgt cactcccacc
     1621 gtcggggcct cgttcctgcg ccccgagact tccttccgat ctccgtcgac cgttgccggg
     1681 gacgccgaga aacggtccgc cgccggggac gggaaacccc cggcccccta cgaggtgttc
     1741 atcggggaaa ccgtcagtgt tctctattcg cccgtaacga tccgggggga gaaagtcctg
     1801 gacggcattt tgggccggcc cttgccctcg gtcgttccat cctccctgga atcccttccc
     1861 ttcgaccgct tcccggatga gctggtgact ttctctccca tgctttgtcc ccggtgcggg
     1921 tgggacctcg agggagaaaa agacagcgtg gttttgctgt gtcgcaattg ccgttcggcc
     1981 tgggcttccc gggggtccgg gctggagagg gtcgactttt ccgtcatggt cgaacccaaa
     2041 gaggcgcccc tctaccttcc cttctggcaa attcgggcga ggttttcggg gattcgactc
     2101 gattcctacg cggacctggt ccgcctggcc aatctgccca aggccgttca gccggaatgg
     2161 gaagaccgca aggcccgtct ctggatccct gccttcaaaa tcgctcccca tctcttcctg
     2221 cgcctgggaa gggccttgac cctcttccaa ggagaagggg atcccgaagc tccccttccg
     2281 tcctcggggc tccatcccgt gagcctgccc ccggaagaag cggcggagag tctcgtgatc
     2341 accgtggccg cattcggggt gcccaagaaa accctcctgc cgaagcttcc cgaaatccgg
     2401 gccccaatgg aagggtacag cctgtgctac gttccctttc gggtgacttc cgcggaatgc
     2461 atccagccga aaatcgggtt gagcctgaac cgaaatgccc tcgacctggg ccgaaaaatc
     2521 taggcaggcc cggcaacgga cggtttcatt ctcttcctgc gggggtcggg tcgagtccca
     2581 cggttttgcc acgggtcccg ccgcacccca taaagtacca ttgggcttcc cagctccgga
     2641 gaagacccgc cagtttctcg cgaacctccg taacgtccga gctggtaatc gaggacaggg
     2701 actcggcggc atccagggcg tcccggatcg ctcgctccgc atccctgcgg tctcgcactt
     2761 ctcgcagctc catgtgccgc acatggaata cggcccgctg caggcggcac aggtccattc
     2821 tcgagaccga aagggagccc agcatttcca ggatgtcccg ggccagccgg gccttgtccc
     2881 cgaggacatc gagtgtcagc tgcagggttt gatccggggg cccgccgccg ggacccggga
     2941 agatccgggt ctcgagacaa tagcttccct cttggtcggg ggtccgggcg tagaaaagca
     3001 gggtcttttc ctcgtcgcca tccaggtgaa gatcaatgcc ccacgggttc tgcgcaaccc
     3061 attccccggg cgccggatcc cacaaccgga tctcctgcgg gtaactctcc tcgatgagca
     3121 gctcccggct tgtcccggag ctcatcagct cgatcttcac cgggatgagc tcgtttggaa
     3181 ggaagccgac cccttcccgt tcccggtgca catgggtgag gcagtcttcc accagggttt
     3241 tcagggtttc atgggtgtcg tcgtttagca ggttgtggac ggtgacgccg accctcagag
     3301 ggtccccttc ctcggggtcg gacagggaga aaaggatgtc cgtggaagag acggcaaggt
     3361 cgggaagggt gggcggcaca atgtgccggg aaaggatcgt ggagttgttt tcttcgttgg
     3421 attcctcgat gaggtcctcc gggtccacga ggatatggag gtagttcctg cccgactggc
     3481 cgaaggtgtt ccaggggatc tccgccaggg tgctcgcccc gggttcgagg gcggccttgg
     3541 tcacggaacc gaggaaggtg ccgtccgcct gaggagcgtc gtcaaagaag gagaccgtca
     3601 cgtcggaggc gctcgaggtc cccaggttgc gaaccagagc caggatcgag gccggttgac
     3661 cttcgatggg cccggcaggt acaagagcga cgtcttcggc agagacggcc aggccgggaa
     3721 gcatggtgga cgggggaccg ctcccgccca gggagaacga ggcgggagcc gtgttgttgt
     3781 cccgtcgaca ttcttggacc tttccctggg ggtccgcgac gacccggaaa acgtgggtcc
     3841 cttcgaccgg gtcgttccat tggacggaaa cctcctcgaa ttcccccggg gccggggtct
     3901 tggtggtgag ggtcgatccc agcagggccc ccgcgggggg cgggtcttct ttgtggaagg
     3961 cgacttccac cccggaatcc agggctacgg ccccccccgt tgccgatcct tgcaaaaacg
     4021 gccacccggg ccgggtacgc cgtccggtcc accgaaagca gagatgccgt gatgtccggg
     4081 gaccccaggg tttcgtgggg aagaagcgtg ttgcagcgat aggtgttgta ctgttcccaa
     4141 ctgttggctt cccggacggg gatggtgccg tcgtccccaa cgttggtgat gtgataggcg
     4201 tgctggttcc agatgggacg ggtcttagcc caggagttcc cggcgtcttc aaacactcga
     4261 attcccgtct ggctccccca cgcgtaattg ttcgcagtga ccaccaactc cgcccggcgg
     4321 tctccgtcca cgtcggccac aatggggtat tcctgggccg ttccattgga gttgggcacc
     4381 tgaaagagaa tcgcgccgtc cgttccccgg tagatgcgca gggtgtgctc gtccccgtag
     4441 acgacctcat tttgcccgtc tccctggaaa tcgaacacgg tggacccggt gctgtcggaa
     4501 ctggtgtctt gggtgacgct ttgccacagg atggtgccgt ccgccttgta caccacgtac
     4561 cggcatgccc cagccgtccc gatctcgggt gctccgtccc cgtccatgtc ggcaacggcc
     4621 gggggcccac cgaagcctcc ccccggaatg ggaacgggtc cccacttcaa atcgcccgta
     4681 tgatcgagaa catgcacggc gccgttggtc accggcacga tttcgggaaa ggggtcctca
     4741 tccaggttgg ccactgcgtt gaacccgttg gaaagcttcg tcttcatcca gaggatactt
     4801 ccatcgttcc ggtaaaccgt gttgccgccg atcacctcgg gaattccgtc caggtcgagg
     4861 tccgccacga tggagagggg acagggcagg tatccgccgg ttccgctcag cgcatcacac
     4921 atccggatgg gcggcggcag ctgagcaagc ctgcggg
//
LOCUS       DAOOVC010000223         4942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9546_length_4942_cov_5.214651, whole genome shotgun sequence.
ACCESSION   DAOOVC010000223 DAOOVC010000000
VERSION     DAOOVC010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9546_length_4942_cov_5.214651"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..738)
                     /gene="gspG"
                     /locus_tag="PLM79_17215"
     CDS             complement(283..738)
                     /gene="gspG"
                     /locus_tag="PLM79_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOV88098.1"
                     /translation="MGVHRHRNSKDRGHPPRGSAGFTLIELLIVMIILGLLAALVAPK
                     MFQKVGSSKQKAAKAQIALLGTALDAYRLDIGRYPSTEEGLDALRKNPGHTGWDGPYL
                     PKDVPPDPWGRAYLYRSPGEHGDYDLVSLGADGQDGGEGENADVNSWDN"
     gene            complement(853..1917)
                     /locus_tag="PLM79_17220"
     CDS             complement(853..1917)
                     /locus_tag="PLM79_17220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOV88099.1"
                     /translation="MSGKKPYRDFNSYLCERFGCRVQKITLDAGLTCPNRDGSLGRHG
                     CIYCNERGSGTGASRSGLSITQQLRSAKSYLAKKYKAKKFLAYFQSFSNTYGPLERLR
                     SLYEEALADEDVVGLSVGTRPDCVSEEVLDCLEAFSAKSLIWMEYGLQSAKDSTLVTI
                     GRGHSVESFADAVRRTCRRGLPICVHVILGLPGETGEDMMNTARFLAALPIQAVKIHL
                     LYVVRGTLLEQWYRWGTYSCLSREEYVSLAARFLELLPPHVIVQRLTGDPHPEELVAP
                     QWAMDKQGNLQALLTHMGEKGLYQGRLFRGGTEQPAGDPLSPTLAGPGAGTCMGRSGE
                     SSGGSGEHVWCRVDTKPRVG"
     gene            2087..3991
                     /gene="mnmG"
                     /locus_tag="PLM79_17225"
     CDS             2087..3991
                     /gene="mnmG"
                     /locus_tag="PLM79_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOV88100.1"
                     /translation="MKKYDVIVVGAGHAGCEAALAAARMGCSTLLLSISLDTVAHMPC
                     SPSIGGIGKGHLVKEIDALGGEMAKVTDQSAIQYRLLNTKKGPAVRGTRTQNDKVRYR
                     TLMKHTLERQAGLDLKQSLVESLVVEGDRLVGVVDQLGVFFSGRAVVLTTGTFLHGLV
                     HIGRARIPAGRAGEFPSNLLADQLRTLGFVLGRMKTGTPARIRRQSIDLSRFAVQEGD
                     ENPRPFSLSTSRIPLTRLPCYIGRTHKGTHELVRSHIHLSPLYNGSIQGVSARYCPSL
                     EDKVMKFPHKEFHQIILEPEGLDTEEIYASGTGNSLPYEIQLGIVRSIAGLEYADLMR
                     PAYAIEYDFVQPTQLHPTLETKRIRGLYMAGQINGTSGYEEAAAQGMWAGINAALKVR
                     GEPPFLLDRSEAYMAVMVDDLVTKGTREPYRIFTSRAEYRLLLREDNADLRLLEKGYR
                     LGLRSHSAYREMIERREAVRAELQRLAKTFIAPSPRLCSLLESKRSPAVEGSVSLDRL
                     LKRPEIGYEDIEAVCAPSTPLPDSVKEQVEIECKYEGYLRRQEAEVKKFRQFEQVRIP
                     EDFPYGDVPGLSNEVRQKLQEIRPLSLGQASRISGITPAAVSILLVYLKRYGQREGGG
                     APARTEGNIP"
     gene            4110..4541
                     /locus_tag="PLM79_17230"
     CDS             4110..4541
                     /locus_tag="PLM79_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOV88101.1"
                     /translation="MTEGQNERDFIATLRGHGLQVTYQRLAIYQALYDNLDHPSAEVI
                     YQQVRKRFPMISLGTVYKTLERFYEVGLVQKVSPVTEVARYDAKVDPHHHMVCLKCQS
                     ITDLDASGLSHKITLPEGHDFRVLRHQVLIQGYCSDCHKES"
BASE COUNT          973 a         1492 c         1490 g          987 t
ORIGIN      
        1 cagggctgga tcctaagtcc agtgcgtctg ccggttccgc cactctcgcc gggaatgtcc
       61 gcaatcggcc atgaacatgt aatacaatca ctttcacgca ccggtgtcaa ttatatcgga
      121 aaaaaaaggc cgaaaatgac cccccggcag tagacccacc ccaccgcgga tgcttccctt
      181 cgggtgtttt cgaggaatgc ggtctgcagc ttttcaggaa gcaggaaagg tttctctctt
      241 gcgtgaagac cccggcaggc gcaccgttac cccggcgttc ggtcagttgt cccagctgtt
      301 gacgtcggcg ttttcgcctt ctccgccatc ttgcccgtcg gctcccagag ataccaggtc
      361 gtagtcccca tgttctcccg gagatcgata caggtaggcc cttccccaag ggtccggagg
      421 aacgtccttg ggcaggtagg ggccgtccca acccgtgtgc ccggggttct tccgaagagc
      481 gtccaggcct tcctcggtgc tcggatacct tccgatatcc agtcggtacg cgtccagggc
      541 ggttcccagc agggcgatct gggccttggc ggccttctgt ttggagctgc cgaccttctg
      601 aaacatcttg ggggccacca gggcggccaa gagccccagg atgatcatga cgatgagcaa
      661 ttcaatgagg gtaaagcccg cgctcccccg gggggggtgc ccgcggtctt tcgagttcct
      721 atgccgatga actcccatgg cccttctccc tgaaaccttc ggtctgaatt gaaaaggccc
      781 ttgcgggtgg ttttggcgaa cttgcggtcg tctccgtgcc atggggccgg ccgccgtgcc
      841 gtagaagtgt atttaaccga cccggggctt cgtgtcaacg cggcaccaaa catgctcccc
      901 cgaccccccg gaggattccc cggatcttcc catacaagtc ccggccccgg ggccggcgag
      961 ggtgggtgaa agcgggtccc cggcgggttg ttccgtcccg ccccggaaga gccttccctg
     1021 gtacagtccc ttttctccca tgtgggtcag aagggcctga aggtttccct gcttgtccat
     1081 ggcccattgg ggagccacca gttcctcagg gtgggggtct cccgtcagcc gttgaacgat
     1141 cacgtgaggc ggaagcaact cgagaaaccg ggccgccagg gacacgtatt cctcccggga
     1201 caggcacgag tacgtccccc accggtacca ctgctcgagg agagtgccgc gtaccacgta
     1261 aagcagatga atcttaaccg cctggatggg cagggcggcc aggaagcgag ccgtgttcat
     1321 catgtcctcg ccggtttccc cgggcaatcc cagaatcaca tggacgcata tgggcaaacc
     1381 gcggcggcag gtgcgccgga cggcgtccgc aaaggattcc accgaatggc ctctgccgat
     1441 ggtcacaagg gtggagtcct tggccgactg gaggccgtat tccatccaga tcagggattt
     1501 cgcggaaaag gcctcgaggc agtccaagac ctcctcggaa acgcagtccg gccgggtccc
     1561 gacggacagg cccaccacgt cttcatcggc gagggcctcc tcgtacaggg acctcaaccg
     1621 ctccaggggc ccgtaggtgt tgctgaagga ctgaaaatag gccagaaatt tcttggcctt
     1681 gtactttttt gcgagataag attttgcgga tcgcagctgt tgggtgatgc tcaacccgga
     1741 gcgggacgcc ccggttccgg acccgcgctc gttgcagtaa atacagccgt ggcggcccag
     1801 ggacccgtcc cggttggggc aggtcagtcc cgcatcgagg gtgatctttt gcacgcgaca
     1861 gccgaaccgt tcgcacaaat aggagttgaa gtctcgatag ggtttcttgc cggacatggc
     1921 gccacgcaga ggaaacggac cgcttggtgc gggatcgcac acaccctcat ctccaacgga
     1981 cgaccctccg gagtttggga ggatctacag ccccgggttc cgccttggga agctcagaat
     2041 aacacggatg gacggactca tccaataagg aaggcttcaa aagcttatga agaagtacga
     2101 cgtgatcgtc gtgggtgccg ggcatgccgg atgtgaagcg gccctggccg ccgcccgcat
     2161 gggatgctcc acccttctgc tctccatttc cctagatacg gtcgcacaca tgccctgttc
     2221 cccttccatc gggggcatcg gaaaaggcca cctggtaaag gaaatcgatg ccctgggcgg
     2281 ggagatggcg aaagtgacgg accagagcgc catccagtac cgattgctga ataccaagaa
     2341 gggccctgcg gttcgtggga ctcggaccca aaacgacaag gtccggtatc gcactctgat
     2401 gaagcatacc ctcgagcggc aagccggcct ggacctgaag caatccctgg tggaatccct
     2461 ggtggtggag ggagaccggc tggtgggggt cgtagaccag ttgggggtgt tcttttcggg
     2521 aagagccgtg gtgctgacca cgggcacttt tttgcacggc ctggtccata tcggccgggc
     2581 tcggatcccc gcggggaggg ccggagaatt cccctccaac cttctggccg accagttgag
     2641 gactctcggg tttgtcctcg gtcgaatgaa aacgggcacc cccgcccgga tccggcggca
     2701 gagcatcgac ctgagccgct tcgccgtcca ggaaggggat gaaaaccccc gcccgttttc
     2761 tctttccacg tcccggattc cccttacccg gctaccctgc tacatcggca ggacccacaa
     2821 ggggacccat gagctggttc gaagccacat ccacctgtct cccctgtaca acgggtccat
     2881 tcagggggtt tccgcccggt actgtccttc cctggaagac aaggtgatga aattccccca
     2941 caaggaattc caccagatca ttctcgaacc cgaagggctc gacaccgagg aaatctatgc
     3001 aagcggaacg ggcaattccc tgccctacga gattcagttg gggatcgtcc gttccattgc
     3061 cgggctcgag tatgccgacc ttatgcggcc ggcctacgcc atcgagtatg atttcgtcca
     3121 accgacccag ctccatccga ccctcgagac caagcggatc cgcgggctct acatggcggg
     3181 gcagatcaac gggacctccg ggtacgagga agcggccgcc caaggcatgt gggccgggat
     3241 caacgccgcc ttgaaggtcc ggggggagcc tcctttcctg ctggatcgct ccgaggccta
     3301 tatggcggtg atggtagacg atttggtgac caaggggact cgggaacctt accgcatctt
     3361 tacctcccgg gcggaatacc ggttgctgct tcgagaggac aacgccgatc tgcgccttct
     3421 cgagaaaggg tatcgactgg ggcttcgttc ccactcggca tatcgtgaaa tgatcgagcg
     3481 tcgggaagcc gtccgggcgg aactccaaag gctggccaag acctttatcg ctccttcccc
     3541 ccggctgtgt tccctgttgg agtccaaaag gtcccctgcg gtcgaggggt ccgtttccct
     3601 ggatcgcctc ctgaagcgcc ccgagatcgg ttatgaagac atcgaggccg tttgtgcccc
     3661 ctcgactcct ctccccgatt ccgtgaagga gcaggtggaa atcgagtgca aatacgaggg
     3721 gtacctgcgc aggcaggagg cggaagtgaa gaagtttcgg cagttcgagc aggtgaggat
     3781 ccccgaggat ttcccttatg gggacgtccc cgggttgtcc aacgaggtcc gtcagaaact
     3841 gcaggagatt cgccccctga gcctcggtca ggcctccagg atttccggaa tcacccccgc
     3901 agccgtctcc atcctcctgg tttacctgaa gcgatacggc caaagggaag gagggggggc
     3961 tccggcaaga acggagggca acattcccta acatcactat aaaaaaaagc ttgtgcttac
     4021 cctgataact attctagaat aatagcagag tgagtatcat gccgatctta tgctgttgag
     4081 atatcgtttt cgcttggaaa ggggatgaaa tgacggaggg gcaaaacgaa agggatttca
     4141 ttgccacgct acgaggccat ggactgcagg tcacctatca acgtctcgcg atttaccagg
     4201 cgctctacga caacttggat cacccaagcg cagaggtcat ttaccagcag gtgagaaaac
     4261 ggtttccaat gatttctctg ggcacggtgt acaagaccct cgagcgcttc tacgaggtcg
     4321 ggttggtgca gaaggtgagt ccggtcacgg aagtggcccg ctatgacgcc aaggtggacc
     4381 ctcatcacca catggtgtgc ttgaagtgtc agagcatcac ggacctggat gcttcgggat
     4441 tgagccacaa gattacgctg cccgaagggc atgactttcg agtcttgcgg catcaggtgc
     4501 tcatccaggg ctattgttcc gattgccata aggaatccta aggccgaggt tttcccgagc
     4561 ggcaggcaag ggggagaact gcggggagtc gttctcgtgg ggggggtgtc ggcgaaacgg
     4621 atccccgaac ggatgggtgt gtcccggcgc cgggaccttc tgcccctggg gtgagcctcg
     4681 cctccgtcca aggaaacccg ctcctgcctc ccggggatct tccctttccg tgccgcagga
     4741 tctgcggcct catcgcctcc cctcaattct ccggtccgac catctcttcc aaccatcgac
     4801 ccgagcgagg tgcctcccgg gacccgttct cttcccacgg ccggtttgtc cctgtacaga
     4861 agggtgcggg tatgctatga agaaaggtta attgcgcgct ccatttgccc gtttggagcc
     4921 cgtgatcccg gcgcttgtga gc
//
LOCUS       DAOOVC010000224         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9587_length_4929_cov_5.187936, whole genome shotgun sequence.
ACCESSION   DAOOVC010000224 DAOOVC010000000
VERSION     DAOOVC010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9587_length_4929_cov_5.187936"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLM79_17235"
     CDS             complement(<1..540)
                     /locus_tag="PLM79_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88102.1"
                     /translation="MDSFPPWMLWAWEKPERLGFIDVNRVGVAFLSRTCVLMGGRVVV
                     RPRMQPLRIPPGSALMAVVRIETDRRSPPDLGPGQMDELVRAALEAARRPGLGGIQVD
                     FDARVSQRRFYREFIERLRRGLPDSMALSVTALASWCLYDDWMTDLPVDEIVPMVFRM
                     GADTDRVRRHLAAGSGFGCP"
     gene            complement(1088..1654)
                     /locus_tag="PLM79_17240"
     CDS             complement(1088..1654)
                     /locus_tag="PLM79_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88103.1"
                     /translation="MKEQECDVRCRILAEAIREAGAAGRLVGKGTLSARLEERGFGVP
                     GGRDGPDLEALLAMTLAANEDLAAFPSHTGETLYHCPAVLSRTYASILDRKSSPILLI
                     AEEVRRNSAESLRPVPLDLFGEAPFDLSPQEIQAALQSMAGSSEFEDVASVLTDAGAV
                     YLFSTRYLEPGYAAFLAEEAETGPFMNP"
     gene            complement(1651..3768)
                     /locus_tag="PLM79_17245"
     CDS             complement(1651..3768)
                     /locus_tag="PLM79_17245"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HOV88104.1"
                     /translation="MPTTTGGFAGKILRVDLSTGKIGLDDTVERYRDVLGGTGIGYRV
                     MWEEVPAGTAPFDAANKLVFATGVLAGTSVPCSGRTAITTLFPSCWPKALTASGHMGG
                     RFASKLKYAGYDGIIIEGKADKPVWLFIRDGRVEIRDAGHLWGTGIRRTTLEISREMG
                     ADCVVAAIGQAGENRVPLSLIVNSVSHSAGGVGAVMGAKNLKAIGIQGSGSVRIAGDK
                     VEWERLIKFHLSILGGNNQHVVPAFPHPHSEYYHPNSRWVGAPGKRWGGSDSPVEING
                     GLLDPNRIAFRTNSAAFFLGDQAWKHTVRGNGCSGCPIRCHTILKVPAVAAKYGIRDT
                     AQNTCVGLLFGWAFFKSLQGNRRTPAALEACMVGMNLADDLGIWSNYHQLQRDLIKLY
                     SDGTLKSKIGSQEYASYSWDKYESADPQFLFEIIPRIAMAQGELGRALGLGTGHLLEQ
                     WSIPEEEWTRDLSSGYWKMGHARHHANDVDGQCGVIINTQYNRDAQCHSHTNFVQNGL
                     PLEVQKKLAAEIWGSPDAVDGVGNYTPMNIHKARRARWSLIRKELHDTLGLCNWMGPW
                     VASPIKERGYSGDDSLESRLCSTATGQQIGREELDLVGERIFTLHRALTVRDMGEVDM
                     RTRHDLIPPYVFQDPKGAEPFTRGTIRMDPRDMAKAMDLFYEILGWDQATGAPTAETY
                     ARLGLQSVARGLAKKKLLPGVRG"
     gene            complement(3897..4634)
                     /locus_tag="PLM79_17250"
     CDS             complement(3897..4634)
                     /locus_tag="PLM79_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV88105.1"
                     /translation="MSWETEPKGIEEEGRAAGFAVGLSRRRFLFLSGGAVTSLAAMSI
                     GRAWGADAPVVILVNARGLLLADPTRCVGCQRCELACTEFNDGRAQPSVARIKVDRNL
                     NFGPEGPGDVRGTQGSWGIGLFQPDTCRQCPHPVPCATACPANAIQADPETRARVVNA
                     AQCTGCRLCLRACPWNMMSFDDESGKATKCFLCHGRPKCVEACPSGALRLVPWRDVTH
                     EAGLRTAVLPAIPPEAVKGCIECHVGG"
BASE COUNT          869 a         1598 c         1582 g          880 t
ORIGIN      
        1 ggggcaccca aaacccgatc ccgcagcaag gtgccttcga acccggtccg tgtcggcacc
       61 catgcgaaac accatgggaa cgatctcgtc caccggcagg tccgtcatcc agtcgtcgta
      121 caggcaccac gaggccaggg cggtgacgga aagcgccatg gaatcgggca ggccccgccg
      181 aagcctctcg atgaattccc ggtagaatcg tctctgggac acccgtgcgt cgaaatccac
      241 ctggattcct ccaagacccg gccttcgagc cgcttcgagg gccgcccgca ccagttcgtc
      301 catttggccg gggccgaggt cgggtggaga acggcgatcg gtctcgatcc tcaccaccgc
      361 catgagggcg ctccccgggg gaatgcgaag aggctgcatc cggggccgca ccacaacccg
      421 gcctcccatt aggacacaag tcctgctgag aaacgccact cccacccgat tcacatcgat
      481 gaacccgagc cgttcgggct tttcccaggc ccacagcatc cacgggggaa aggagtccat
      541 gcggccgctt cggtaggcga ggttcccagt ggtcccggac ggagttcctt ttggggagga
      601 tcgaggttcg actgcaccgg caagtcccct cagcagagtc ccccatgcgc cgtcatgccc
      661 gtgaaagaca ctccgtgacg cctcgtttgg gattccgacg ccggcccccg ggagcggctc
      721 atccgaggac ccggcgggca tacgttccac cggtgctggg ccaaacacca acaagaggaa
      781 ccaggggatc gcccatttga gctctccggg aatcattctt ctaaaccaca cgattcctga
      841 aagtcattgc ccatccgacg gcgctccctg cgggcggcaa caaagcccgc cctttgaggc
      901 cgtacaacgg aaaaaatcgc tcatcttggg aataggaccg cgaaggaaaa gacccggagc
      961 cgccgagggt cttcctcccc tcccttcctg aggccgcccc cggaacccgt cccgggttcg
     1021 agggccgggc ggggtcgcga ggcccccctg tgagcttgtc cgaaatcggt ctttcgccgg
     1081 ggaaccttca ggggttcatg aaaggccccg tctccgcctc ttcggcgaga aaggccgcgt
     1141 agccgggctc caggtaccgg gtggagaaga gatacaccgc tccggcatcc gtgaggacgg
     1201 atgccacgtc ctcgaactcg gagctcccgg ccatggattg aagggcggct tggatctctt
     1261 gggggctgag gtcaaaagga gcctctccaa aaaggtccaa gggcacgggc cgcaaagatt
     1321 cggccgagtt cctgcgaact tcttcggcga tcaagagaat gggcgagctc ttccgatcca
     1381 agatcgaggc atacgtccgg ctgagcacgg cagggcagtg atagagggtc tcgccggtat
     1441 ggctcggaaa agcggccagg tcttcgttgg cggccagggt catggccagg agggcctcca
     1501 ggtccgggcc gtcccgcccg ccggggacgc caaagcccct ttcctcgagg cgcgccgaca
     1561 gcgtcccctt tcccacaagc cttccggccg cccccgcttc ccggatcgct tcggccagga
     1621 tccggcaccg aacatcgcac tcctgctcct tcatcccctt acccccggca ggagtttctt
     1681 tttcgccagc ccccgggcta cggactggag ccccaagcgg gcgtaggtct cggcggtggg
     1741 cgcccccgtg gcctggtccc atcccaggat ttcatagaag aggtccatgg ctttggccat
     1801 gtcccgggga tccatgcgga tggttccccg ggtaaagggc tcggctcctt tggggtcctg
     1861 gaagacgtag ggagggatga ggtcgtggcg ggtacgcatg tccacctccc ccatgtcccg
     1921 gacggtgagg gcccggtgca gggtaaagat ccgttcgccc acgaggtcca gttcttcgcg
     1981 gccgatctgc tgtcccgtgg ccgtgctgca cagcctcgat tccaggctgt cgtcccccga
     2041 atagccgcgt tccttgatgg gagaggccac ccagggtccc atccagttgc acaggcccag
     2101 ggtatcgtgg agctccttgc ggatcagcga ccacctggcc cggcgcgctt tgtggatgtt
     2161 catcggggtg tagttgccga ccccgtccac ggcgtcggga gatccccaga tctccgccgc
     2221 cagtttcttc tgcacctcga ggggcaggcc gttctggacg aagttcgtgt gggagtggca
     2281 ctgggcgtct cggttgtact gggtgttgat gatgaccccg cactggccgt ccacatcgtt
     2341 ggcatggtgg cgggcatgcc ccatcttcca atatccgctc gacaggtccc gggtccattc
     2401 ttcttccggg atggaccact gttcgagaag gtgcccggta cccagcccca gagcccgccc
     2461 cagttccccc tgggccatgg cgatccgggg aatgatttcg aacagaaact ggggatcggc
     2521 gctctcgtat ttgtcccagg aataggaggc gtactcctgg gaaccgatct tggacttgag
     2581 agtaccgtcg gaatacagct tgatgaggtc ccgttgaagc tgatggtagt tggaccagat
     2641 tcccaggtcg tcggccaggt tcatgcccac catgcaggct tccagggccg ccggggtccg
     2701 acgattcccc tggagcgact tgaagaaggc ccagccgaag agcagtccca cgcacgtgtt
     2761 ctgtgccgtg tcccgaatgc cgtacttggc ggccacggcg gggaccttga ggatggtgtg
     2821 gcagcgaatg ggacacccgg agcagccgtt tccgcgtacc gtgtgtttcc atgcctggtc
     2881 ccccaagaag aacgcggcgc tgttggtgcg aaaggcgatc cggttcgggt caaggaggcc
     2941 gccgttgatc tcgacggggg agtcggagcc tccccaacgt ttgcccggag cccccaccca
     3001 gcgggagttg gggtggtagt attccgaatg cggatgggga aaggcgggca ccacgtgctg
     3061 gttgttgccg cccaggatgg agaggtgaaa cttgatcagg cgctcccact cgaccttatc
     3121 cccggcaatg cgcacggatc cgctcccctg gatgccgatg gccttgaggt tcttggcccc
     3181 catcaccgca cccactccgc ccgccgagtg ggagaccgag ttgacgatca gggacaaggg
     3241 cacccggttt tccccggcct gaccgatggc cgccacgaca caatcggcac ccatctcccg
     3301 gctgatctcg agggtggtgc ggcggatgcc cgtgccccag aggtgcccgg cgtcccggat
     3361 ttccaccctt ccgtcacgaa tgaagagcca cacgggtttg tcggctttgc cttcgatgat
     3421 gatcccgtca tagcccgcgt acttgagctt ggaggcaaac cgtccgccca tgtggccgct
     3481 tgccgtgagg gccttgggcc agcaggaggg aaacagggtg gtgatcgctg ttcggccgct
     3541 gcacggcacg ctggtcccgg ccaggacccc ggtggcaaac accagcttgt tggcggcgtc
     3601 gaagggggcg gtcccggcgg ggacctcttc ccacatcacc cggtagccga tgccggtgcc
     3661 tcccagcacg tcccggtacc tctcgacggt gtcgtcaaga ccgatcttac cggtggaaag
     3721 atccacccga aggattttgc ctgcaaaacc gccggtagtt gtgggcatgc gtgtctcccc
     3781 ggaatgagcg atcgaacgga ggaaggaatc caaaggaatc gaaaaccccg gcgtcgcacc
     3841 cgtccgaaga ccggccggaa gtctcgaccc cgggtctggc cgggccgaat cggtggtcac
     3901 ccgcccacgt ggcactcgat gcatcccttg accgcttcgg gggggatggc gggcagaacg
     3961 gccgtgcgca aaccggcttc atgggtgacg tcccgccagg ggacgaggcg aagcgccccg
     4021 gaagggcagg cctcgacgca cttgggacgg ccgtggcata ggaagcactt ggtggctttt
     4081 ccgctttcgt cgtcgaagct catcatgttc caagggcatg cacgaaggca taagcggcag
     4141 ccggtgcatt gggccgcgtt cacgacccgg gcccgggttt ccgggtcggc ctggatggcg
     4201 ttggcgggac aggccgtggc gcagggcacg ggatgcgggc attgacggca ggtgtccggt
     4261 tgaaagaggc cgattcccca gctgccctgg gtccctcgta cgtcgccggg accctcgggg
     4321 ccgaagttca ggtttcgatc gaccttgatt cgggctacgg aaggctgggc acggccgtcg
     4381 ttgaattccg tgcaggccag ctcacagcgc tggcacccga cgcaccgggt gggatcggcc
     4441 aggagaagcc cccgggcatt gacgaggatg acaacggggg catcggcccc ccaagcccgg
     4501 ccgatgctca tggccgcgag gctggtgacg gccccgcccg aaagaaaaag aaatcgtctt
     4561 cgtgaaagac cgaccgcgaa accggccgcc ctcccctcct cctcgatccc cttgggttcc
     4621 gtttcccaac tcacggaaat cccctctcct cagaccaatg tttgtgccgc ctgtttccat
     4681 cccggggacc acgcaaccgg gccgaagact gcgccctctc gggatctgca gtttgggcat
     4741 taaaccacgg tcgcccttcc cagtaaagac cccatccggc ggccgattct tttccggggc
     4801 gtgagttgtg tgcgtcaagg tctgctggct cgctcaaggc atttgggtat gttccgattc
     4861 gcaaattccg atgagcaagg gccgcggaga atgggtccaa ccgtgactcg tcggaggcgg
     4921 gacgttggc
//
LOCUS       DAOOVC010000225         4902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9648_length_4902_cov_5.456158, whole genome shotgun sequence.
ACCESSION   DAOOVC010000225 DAOOVC010000000
VERSION     DAOOVC010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9648_length_4902_cov_5.456158"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="PLM79_17255"
     CDS             <1..1507
                     /locus_tag="PLM79_17255"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HOV88106.1"
                     /translation="RSLRGGGGVPGFRTLLKSPNERGEAMIIPVILAGGSGTRLWPFS
                     RELYPKQLLPLVDDRTLLQNTVLRLSGMEGIADPIVLCNENHRFLVAEQFRAVNVRPA
                     SILLEPVGRNTAPAVAVAALHAAAADEQVVLLVLPADHHIEDVRAFQDAVQVGARFAR
                     EDYLITFGIVPDAPETGYGYIQGGDRVRTSGGDLEAVKIRKFVEKPDPKTAREYVDSG
                     SYFWNSGMFLFRASRILAEMAQFAPAVVEACRVALEKGVPDLDFYRLDSEAFHACPGD
                     SIDYAVMEKTQSGVMIPLSAGWNDLGSWDALWKAQPKDERQNVSLGDVLVHDVERCYI
                     HSTSRMVAAIGLKDHIVVETSDAVLISSWDRVQEVKSLVQRMKNGDREEALTHRKVYR
                     PWGSYEGLNLGDRFQVKRLTVKPGAKLSLQRHHHRAEHWVVVHGTALVTNNDKQFLLT
                     ENESTYIPLGAVHRLENPGKIPLEVIEIQTGSYLGEDDISRLEDVYRRVSN"
     gene            1714..2850
                     /locus_tag="PLM79_17260"
     CDS             1714..2850
                     /locus_tag="PLM79_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOV88107.1"
                     /translation="MHAVPPGVRGGWIHCDPEGEPAGSGLRNPFGTDSPGCNRIVINL
                     SYLPGAIENYFRDGTRWGCQIAYSYEGRIVDGKFVGSALGSAGGLKKVQDFSNFFDDT
                     FLVVCGDALIDLDVGKLREFHRSRGAVATIALKEVPLSEVYRYGVVAVDETGRILRFQ
                     EKPRPEEAVSTTVNTGIYMFDPAVFEFIPSGVEYDIGGQLLPALVQAGAPFYGISPPF
                     RWVDIGSIPDYWAATRLVMSGKIRGISLPGREIAPGIYTGINLRLNPEKVNLTPPVYI
                     GSSTFIGDGATIVGPTVIGANCIIEGGAILKQCVIDDHVKIADIADISEKIIFSGNYI
                     DPKGQYLDLAEADMEWVITDARKEINISETHLLLKELAHGAGPG"
     gene            2940..3683
                     /locus_tag="PLM79_17265"
     CDS             2940..3683
                     /locus_tag="PLM79_17265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOV88108.1"
                     /translation="MPGRDDRSDSELLEQLYRKFARGGTLIARIRGTAYFFFKKYSWI
                     AVTESTYFIKRLLDVCVSAVMLVVLSPIFLVTALAIKIDSPGPAFYKQTRVGKWGRLF
                     TMYKFRSMILGADKMKQQLLDQNESGGVIFKMKRDPRITRVGRIVRKLSIDELPQLWN
                     VLKGDMSLVGPRPPVPGEVAQYEYTDRRRLDAIPGITCIWQVSGRSEIDFKGQVRLDV
                     QYIENQSFWGDIKLLLKTIPAVLFGKGAY"
     gene            3683..3907
                     /locus_tag="PLM79_17270"
     CDS             3683..3907
                     /locus_tag="PLM79_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88109.1"
                     /translation="MFSETVVIPGIPVDNLGMDEAVERIFAMVEDYRRDRRPRLVTTV
                     NVDFVVNTPTRRLSGLRRLGSARRMIEGTP"
     gene            3904..>4902
                     /locus_tag="PLM79_17275"
     CDS             3904..>4902
                     /locus_tag="PLM79_17275"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HOV88110.1"
                     /translation="MKAVILPTAKTEDLAPLTTWMPEFLLPVVNKPVVEHLIELLVRH
                     GVKEILLVLKHMPYETEQYFGDGSRWGVRLSYSLLTRYRGIADALGRTESSRLEGPLI
                     CLPGDLVTDLDLCGFMDARCRGPGSIVSARPVARSSDPGLSGASAGEAQGPDACPLFI
                     SEEALPSVSALGIPGTPGSPGEGVSVYPVTCGYERIRSPADLVRVNRRVIEGGFKGIL
                     LPGREIEPGIWVGRHCRIHPRARLEAPVLIGSHCDIQAGSVVGPGSVVGDHVIVDEGA
                     SVQESVVLDRTYVGVHTRIHDALVRKNWMLQTPTMLEVYLVYFMIMADPEKGTLSTQ"
BASE COUNT          970 a         1485 c         1522 g          925 t
ORIGIN      
        1 gagaagcctt cgagggggag gtggggtccc gggattccgg acgcttctga aaagcccaaa
       61 cgaacgaggt gaagccatga tcattcccgt cattctggcc ggtggttccg gaacgaggct
      121 gtggcccttt tcgcgggagc tctaccccaa acaactgctc ccgttggtgg acgaccggac
      181 gttgcttcag aataccgtct tgaggctcag cggcatggaa ggcatcgccg atcccatcgt
      241 cctgtgcaat gaaaaccacc gcttcctggt tgccgagcag tttcgggccg tcaacgtccg
      301 gcccgcctcc atccttctcg aaccggtggg gaggaacacg gcacccgccg ttgccgttgc
      361 cgcccttcat gctgccgccg ccgatgagca ggtcgtcctc ttggtgcttc ccgccgacca
      421 tcacatcgag gatgtccggg ctttccagga cgccgtgcag gtgggggccc ggtttgcccg
      481 ggaggattat ctcattacct tcggaatcgt cccggacgct cccgagaccg gctacgggta
      541 catccagggg ggggaccggg tccggacctc gggaggcgac ctcgaggcgg tgaagattcg
      601 caagtttgtc gaaaaacccg atccgaagac ggcccgggag tacgtcgatt cggggagtta
      661 tttctggaac agcgggatgt tcctgttccg ggcctcgaga atcttggcgg aaatggccca
      721 attcgcccct gccgtggtcg aagcctgtcg agtcgctctc gagaagggag tccccgacct
      781 ggatttctac cggttggatt ccgaggcctt tcatgcatgt cccggcgatt ccatcgacta
      841 cgccgtgatg gaaaagaccc aaagcggggt catgattcct ctctcggcgg gctggaacga
      901 cctgggatcc tgggacgccc tctggaaagc ccagccgaag gacgaaagac agaatgtttc
      961 cctgggcgac gtcctggtcc acgacgtgga acggtgctac atccattcca ccagtcgcat
     1021 ggtggcggcc atcgggctca aagaccacat cgtcgtggag acctcggatg cagtcctgat
     1081 ttcgagctgg gatcgagtcc aggaagtcaa gagcctggtg caaagaatga aaaacgggga
     1141 tcgcgaggaa gccttgaccc accgaaaggt ctatcgtccg tggggttcct acgaaggtct
     1201 caacctgggg gaccgcttcc aggtgaaacg cctcaccgtc aagcccgggg cgaagctttc
     1261 cctccagagg catcatcacc gggcggaaca ctgggtggtc gtgcacggta cggccctggt
     1321 gaccaacaac gacaagcagt ttctcctgac cgagaacgag tccacctaca ttcccctggg
     1381 agccgttcac cgcctcgaga atcccgggaa gatccctctc gaggtgatcg aaatccagac
     1441 gggaagctat ttgggggaag acgacatctc gcgcctggaa gacgtctacc ggcgagtgag
     1501 caactagggg cccatgctcg caaaggtctt cccggagaga ggaccagagc cgaaaatccg
     1561 cccttgaaga tggattccga agggatggga agtgttttca gccatgtagg cgatacgacc
     1621 ctcccgtgac cccccttgga atgaaattca tgaagccttg tccaagcccg ggggccttac
     1681 cggaccaccg aggaagacgg ccgcaagggc ttggtgcacg ccgtcccccc cggagtacgt
     1741 ggcggttgga tacattgtga ccctgaagga gaacctgccg gcagtgggct ccgaaatcca
     1801 ttcgggaccg attcgccggg atgcaatcga atcgtgatca acctcagtta cctgccgggg
     1861 gcgatcgaga actatttccg ggacggaacc cgatggggtt gccaaatcgc ctactcctac
     1921 gaaggcagga tcgtggacgg caagttcgtc gggtccgccc tgggatcggc cggaggcctc
     1981 aaaaaggtcc aggacttctc gaactttttc gacgacacgt tcctggtggt ctgcggagat
     2041 gccctcattg acctggacgt gggaaaactc cgggaattcc accgatcccg gggagccgtc
     2101 gccaccatcg ctctcaagga ggtgccgctc agcgaggtct accggtatgg agtggtggcc
     2161 gtggatgaaa cgggacgaat tcttcgcttc caggaaaagc cccgcccgga agaggccgtt
     2221 tccaccaccg tcaataccgg gatttacatg ttcgaccccg ccgtcttcga gttcatccct
     2281 tccggggtgg aatacgacat cggcgggcag ctccttcccg ccctggtcca agcaggagcc
     2341 ccgttttacg gcatcagccc gccgttccgg tgggtggaca tcggctcgat tcccgactac
     2401 tgggcggcca cacgcctggt catgtccggc aagatccggg ggatttccct gccgggccgg
     2461 gaaatcgctc cggggattta taccggcatc aatcttcggt tgaacccgga gaaggtgaac
     2521 ctcactcccc cggtctacat cggcagcagc accttcatcg gagacggggc gaccatcgtg
     2581 ggacccacgg tgatcggggc caactgcatc atcgaaggag gcgcgatcct caagcaatgc
     2641 gtcattgacg accatgtaaa gatcgcggat attgccgata tctccgagaa gatcattttc
     2701 agcggaaatt acatcgatcc caagggtcaa tacctggacc ttgcggaagc ggatatggaa
     2761 tgggtcatta ccgatgcaag aaaggaaatc aacatttccg agacccatct cctgctgaag
     2821 gaactggccc acggggcagg tcccgggtag gccgtcgtgc gggcgggggg gacctgccgg
     2881 ggggcatgtt cgggttcgcc gcccggcaag cgtgatggat ccgatcatag gggggtgcaa
     2941 tgcccggaag ggatgaccgg tccgattccg aactgctcga acagctgtac cggaagtttg
     3001 caagaggcgg gacgctcatc gccaggatcc gcggcacggc ttatttcttc ttcaaaaagt
     3061 acagctggat cgccgtcacg gaatccacct atttcatcaa gaggctcctg gatgtctgcg
     3121 tatccgccgt catgctcgtc gtgctctcgc cgattttcct ggttaccgcc ctggcaatca
     3181 agatagacag cccggggccg gccttctaca agcagacccg ggtcgggaag tggggcagac
     3241 tgttcaccat gtacaagttt cgatccatga tcctcggggc cgacaagatg aagcagcaac
     3301 tgctggatca gaacgaatcg ggcggggtca tcttcaaaat gaagcgggat ccccggatca
     3361 cccgggtcgg tcgcatcgtc cggaagctct ccatcgatga gctgccccaa ctctggaacg
     3421 tcctcaaggg cgacatgtcc ctggtggggc cgcgtccgcc ggtgcccggg gaggtggccc
     3481 agtatgaata caccgaccgg cgacgcctgg acgccattcc gggcatcacc tgcatctggc
     3541 aggtgagcgg ccgcagtgaa atcgatttca agggccaggt ccggttggac gtccagtaca
     3601 tcgaaaacca gtccttttgg ggggacatca agctcctgct caagacgatc cccgcggtgc
     3661 ttttcggaaa aggtgcctac tgatgttttc cgaaacggtt gtgatcccgg gcattcccgt
     3721 ggacaatctc gggatggacg aagccgtcga gcgcatcttt gccatggtgg aagactatcg
     3781 gcgggaccgg cgcccccggc tcgtgaccac ggtaaacgtc gatttcgtgg tcaacacccc
     3841 cacccggcgc ctgagcggcc ttcggcgcct gggttcggcc cggaggatga tcgaggggac
     3901 tccatgaagg cagtcatcct cccaaccgcc aagaccgagg acctggctcc cttgacgaca
     3961 tggatgcccg agttcttgct tcccgtggtc aacaagcccg tggtcgagca cctcatcgag
     4021 ctcctggtca ggcacggggt caaggagatc ctcctggttc tcaagcacat gccgtacgaa
     4081 acggaacagt acttcggaga cggttcccgg tggggcgtgc gcctgtccta ttccctgctg
     4141 acccggtacc gagggattgc cgacgccctc gggcgcacgg aatcgtcccg gctggaaggt
     4201 cccttgatct gcctgccggg agacctggtg acggacctgg acctctgcgg tttcatggat
     4261 gctcgatgcc gggggccggg aagcatcgtt tcggcccgcc ccgtcgcccg ctcctcggac
     4321 ccgggtcttt ccggcgcctc ggcaggggag gcccaagggc cggacgcctg tccgctgttc
     4381 atctcggaag aggcgctccc gtccgtctcc gcccttggaa tcccggggac ccccggctct
     4441 ccgggggagg gtgtgagcgt ctaccccgtc acctgcggct atgaacggat tcgatccccg
     4501 gccgacctgg tccgggtaaa ccggcgggtg atcgagggcg ggttcaaggg aattctcctt
     4561 cccggaaggg agatcgagcc ggggatatgg gtgggcagac attgccgaat ccatccccgg
     4621 gcaaggctgg aagcaccggt gctcatcggc agccattgcg acatccaagc aggatccgtc
     4681 gtcgggccgg gaagcgtcgt cggcgatcac gtgatcgtcg acgagggcgc ctccgtacag
     4741 gaaagcgtgg tcctggatcg cacctacgtt ggggtccaca cgcgaatcca cgacgccctc
     4801 gtcaggaaga actggatgct ccaaaccccc accatgctgg aggtctacct ggtttacttt
     4861 atgatcatgg ccgaccccga gaaaggcact ctatccaccc ag
//
LOCUS       DAOOVC010000226         4877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9713_length_4877_cov_6.369764, whole genome shotgun sequence.
ACCESSION   DAOOVC010000226 DAOOVC010000000
VERSION     DAOOVC010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9713_length_4877_cov_6.369764"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PLM79_17280"
     CDS             complement(<1..595)
                     /locus_tag="PLM79_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOV88111.1"
                     /translation="MDLGLKGKVALVAGGSQGLGKAVAVRLSVEGAKVAIGALDDAEL
                     PKTVEEIKVLSEGEVIGIPTDVSVAEQARNFVRQAMEHFGTADILVNNAGGPPSRTFL
                     EIDDDLWQQGVRLNLMSAIIMTREVLPVMMEKRWGRIINMTSISVKQPIDGLILSNTV
                     RSAVVGLAKTLSNEMAPYNITVNNVCPGYTMTERVRSL"
     gene            complement(707..1423)
                     /locus_tag="PLM79_17285"
     CDS             complement(707..1423)
                     /locus_tag="PLM79_17285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOV88112.1"
                     /translation="MITSDTMFQGKVTVLQRRDGYRFSIDAVLLAGLTNIRESDRVLD
                     LGTGCGIVLLILAFRRRGAELVGIEIQSELAELARKNVQCNGWEPRIRILQMDFREVA
                     GHLPPRSFDVVVSNPPYRAVHMGRINPEPTRAVARHELAASVGDVFKAARHLLPTGGR
                     LGLIYPAARLARALVESEAHGFSPKELTVIHSRESAPGTLVHVEFRKGGGEELRIAPP
                     FFIYEEQGAYTAKMRSLYEV"
     gene            complement(1452..2780)
                     /locus_tag="PLM79_17290"
     CDS             complement(1452..2780)
                     /locus_tag="PLM79_17290"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOV88113.1"
                     /translation="MAEIVPFRGLRYNPEKVPDLAEVVIPPYDVISPAEQDRFYQIHP
                     HNMIRLELGKKTPQDTEEDNPHTRAGNTLDRWLSESILVREPKPAIYYYELDFTLGEG
                     VRRTRFGFISTMRLAEFTTGMVKPHEKTFQAVKNERLRLMLSCRANLSPVFALYSDPS
                     RAVDRCLRQGKEGEPTLSFADAWGMRHRIWALTGPRVLKEVRELMGDKAIFIADGHHR
                     YETALKYRDIQRERFASARPDAPYEHILIYLANLNQEGMIILPTHRMLRHLGAWEPES
                     FLQKASEFFEVARFPSGPEGERQWRTALESARAAKENAIGLFRQGDDAFYLLKAREEA
                     VSRHLEGKGIARVLHRLDVVVLDQVILRHLMGLDEGFLADENNIHFKHELSEGLDGVR
                     AQVYEAGFFINPTRIEQVQEAANSGLIMPHKSTYFYPKVGSGLVIHTLWP"
     gene            complement(2979..3713)
                     /locus_tag="PLM79_17295"
     CDS             complement(2979..3713)
                     /locus_tag="PLM79_17295"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HOV88114.1"
                     /translation="MMKALRPWLVHEAATDFFYFQNRKYPRTASLEWVGNRYGLSEVE
                     RHLLQRGVFSQETALGRFAKRCRGARWRTEPLYVDGHNVQITVESAVLGRVLLLANDG
                     ALRDLAGQSARFRLTQVSEMAVDLIFRFLEESRPRELFFFFDAPMSHSGILANQYRRR
                     MKTLGIPGDARAVPVPEREFPHDRGVLASSDQAVIEKARWWLDLARCALDAAGIRSVF
                     ADFSYLVLNRRRETGPRGDWLGGIRG"
     gene            complement(3771..>4877)
                     /locus_tag="PLM79_17300"
     CDS             complement(3771..>4877)
                     /locus_tag="PLM79_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOV88115.1"
                     /translation="TVEPDDLAVLQYTGGTTGIAKGVMLSHRNLVANVNQIIAWFQKI
                     ERGKERFVAILPFSHVFGMTVGMNLTLYTGCAAIIIPRFEINEFMKILHKSRPTVFPG
                     VPTIFVAIVNHPKVQDYDLSSIRLCVTGASPMPAEVLRRFEELTGSTIVEGYGLTESS
                     PVTHCNPLLGTRKTCSIGIPLPGTYYRIVDLDLGVRDLPVGEPGELLIRGPQVMKGYW
                     KMPEETARSLRDGWLYTGDIATVDEEGYAFIVDRKKDMIIAGGYNVYPREVDEVLYEH
                     PKVLDAVTVGIPDPYRGETVKAFVVLKPGEEAAEEEIIGFCKEKLAIYKVPKAVEFRE
                     FLPKTIVGKVLRKELRREEIAKAQAQSEVPCEKK"
BASE COUNT          948 a         1571 c         1385 g          973 t
ORIGIN      
        1 ccaggctcct cacccgttcc gtcatggtat atcccgggca cacgttgttg accgtaatgt
       61 tgtaaggggc catctcgttg gacagggttt ttgccagccc gaccaccgcc gatcgcaccg
      121 tgttggacag gatcagccca tcgataggct gctttacgga aatggaggtc atgttgatga
      181 tccttcccca acgcttctcc atcatcaccg gcaggacctc ccgggtcata atgatggcgc
      241 tcatgagatt gagccggacc ccctgctgcc acaggtcgtc atcgatttcg aggaaagtcc
      301 gggagggagg cccccctgca ttgttcacca ggatatccgc agtcccaaag tgttccatgg
      361 cctgtcgaac gaagtttctc gcctgctccg ccacggagac gtcggtagga atgccgatga
      421 cttccccctc cgaaaggacc ttgatttcct ccaccgtctt gggaagctcg gcatcatcca
      481 gcgctccaat ggccactttg gccccttcca cggacagccg gacggcaacg gccttcccca
      541 atccctggct gcctccggca accagggcca cctttccttt caaacccaaa tccattggta
      601 ccctcctcgt tccgaacaaa ccccgccccg acccgacatt ttcccactgc cccccgcgaa
      661 tcccacaccg gtttttccgg gcatgccgga cccgctcgag gggagtttat acctcgtaga
      721 gcgaacgcat tttcgcggtg taggcgcctt gttcctcata gatgaaaaag ggaggagcga
      781 tccggagctc ttctccccct cccttgcgaa attccacgtg cacgagcgtc cccggggcgg
      841 actcccggga atggatcacg gtgagttcct tggggctaaa cccatgggcc tcggattcca
      901 ccagggcccg ggcgaggcgg gcggctgggt agatcagccc caaacgtcct cccgtgggca
      961 acaggtggcg ggccgctttg aatacgtccc ccaccgaggc cgccagttca tgccgggcga
     1021 cggccctggt cggctccgga ttgattctcc ccatatgaac ggcccgatac ggcgggttgc
     1081 tcaccaccac gtcgaagctc cggggaggaa gatgacccgc gacctctcga aaatccattt
     1141 gaagaatgcg aatacgcggt tcccaaccgt tgcattgaac gttctttcgg gcaagctccg
     1201 ccagttcgga ctggatctcg attcccacca gttcggcccc ccggcgcctg aaagccagga
     1261 tcagcaggac gatcccgcac cccgtgccca gatccagaac cctgtccgat tcccggatgt
     1321 tggtgagacc tgccagcaac acggcatcga tggagaagcg gtagccgtcc cggcgttgga
     1381 gaacggtcac cttgccctgg aacatcgtat ccgaagtgat caaagagatt tccttgcctg
     1441 aaagcctacc cctagggcca tagggtgtga atgacgagac cgctcccgac cttggggtaa
     1501 aagtacgtgg acttatgagg catgatcaga ccggagtttg ccgcttcctg gacctgttcg
     1561 atgcgagtgg ggttgataaa gaagccggcc tcgtagacct gagcgcgcac tccgtcgagc
     1621 ccttcgctca attcgtgctt gaagtggatg ttgttctcgt cggccagaaa cccttcgtcc
     1681 aaccccatga ggtgccgcag aatcacctga tccaggacca ccacatccag ccggtgaagc
     1741 acccgggcaa ttccttttcc ctccaggtgg cgggaaacgg cttcttcccg ggccttgaga
     1801 agataaaaag catcgtcccc ctgccggaaa agaccgatgg cgttttcctt ggccgccctt
     1861 gcagattcga gggccgtgcg ccattgccgc tctccctccg gccccgacgg aaaccgggcc
     1921 acctcgaaga actccgaggc cttttgcagg aacgactcgg gttcccacgc tcccaggtgc
     1981 cgaagcatgc ggtgggtggg aagaatgatc atcccctcct ggttcaggtt tgccaggtag
     2041 atcaggatgt gctcataggg ggcgtccggc cttgcggagg cgaagcgctc cctctggatg
     2101 tcccggtatt tcaaggccgt ctcgtagcga tgatgcccgt cggcgatgaa gatcgcctta
     2161 tcccccatga gttcccgaac ctccttcaga acccggggac ccgtgagcgc ccaaatccgg
     2221 tgtcgcattc cccatgcgtc cgcgaaagac aaggtgggtt ccccttcctt tccctgccga
     2281 aggcagcggt ccaccgcccg ggacgggtcc gagtaaaggg caaaaaccgg gctcaggttg
     2341 gcccggcagg aaagcatcag gcgaagccgt tcgttcttga ccgcctgaaa ggtcttttca
     2401 tgcggcttta ccatgccggt ggtgaattcg gccaggcgca tggtactgat gaatccgaag
     2461 cgggtccgac ggaccccctc gcccagggtg aaatccagtt cataataata gatggcgggt
     2521 ttcggctccc gcaccaggat cgactcggaa agccacctgt cgagagtatt gcccgcccgg
     2581 gtgtgcgggt tgtcctcctc ggtgtcctgg ggggtcttct tccccagctc caatcggatc
     2641 atgttgtggg gatggatttg ataaaaacgg tcctgctcgg ccggtgaaat cacgtcatac
     2701 ggcgggatca ccacctcggc caggtcgggc accttttccg gattgtagcg aagccccctg
     2761 aaagggacaa tctctgccat ggacccctct ttctcttccc cgttgccaaa tcgttctcag
     2821 ctccacacga aagcaccaca aagaattcct gcacagcacc ctgagcgcgg ggaaccctta
     2881 gctaacacac ccggcttcgt ttgacaagca acatgggtcg gcacgatccc cggctcctgc
     2941 accggaaccg gcggtcttcg cccgcccccg tcccgacctc atcctcgaat ccccccaagc
     3001 caatcccctc tgggccccgt ctcgcggcgg cgattgagaa ccaggtagga aaaatcggca
     3061 aagacggacc gtatcccggc ggcatccagg gcgcaacggg ccaggtccag ccaccaccgg
     3121 gccttctcga taaccgcctg atcgctgctg gccaggactc cgcggtcgtg gggaaattcc
     3181 ctctcgggaa cagggaccgc ccgggcatcg ccgggaatgc ccagggtctt catcctgcgc
     3241 cggtattgat tggccaggat tccgctgtga ctcatgggag cgtcaaagaa gaagaaaagc
     3301 tctcggggtc gagactcctc caagaatcgg aagatcagat ccacggccat ctcgctgacc
     3361 tgggtcaggc gaaaacgggc cgactgaccg gccaggtccc gcaaggcccc gtcattggcc
     3421 aggaggagta cgcgacccaa gacggcactc tccaccgtga tctggacatt gtggccgtcc
     3481 acgtaaaggg gctcggtccg ccaccgggcg cctcgacacc tcttggcgaa acgccccagg
     3541 gccgtctcct ggctgaaaac ccccctctgg agcaggtggc gctccacttc gctcaatccg
     3601 taccggttgc cgacccattc gagggaggcc gttctgggat attttcgatt ctggaaataa
     3661 aagaaatcgg tggcggcctc gtggacgagc cacggcctaa gcgccttcat catgggacat
     3721 acctgatctt tcggaccgca tcgagaaggg cgtcgggaag gaccacgggg ttacttcttc
     3781 tcgcagggga cctcgctttg tgcctgggcc ttggcaattt cttcccggcg cagctctttg
     3841 cgaagcacct tgcctacgat ggtcttcggg agaaactccc tgaattccac agccttggga
     3901 accttgtaga tggcaagctt ctccttgcaa aacccgatga tttcctcctc agcggcctcc
     3961 tcgccgggtt tgagcaccac gaaggccttg accgtctccc cccgatacgg gtccgggatc
     4021 ccgacggtca ctgcgtccag gaccttggga tgctcgtaga ggacttcatc cacctcccgc
     4081 ggataaacgt tgtagcctcc ggcaatgatc atgtcctttt ttcggtccac aatgaaggca
     4141 tacccctctt cgtccacggt ggcgatgtcc ccggtgtaaa gccaaccgtc tcgaagcgac
     4201 cgggccgtct cctcgggcat cttccagtag cccttcatca cctggggacc ccggatcagg
     4261 agttcccccg gttcccccac cgggaggtcc cggacgccca ggtccaggtc cacgatgcgg
     4321 tagtaggtcc ccggtagagg tatgccgatg gagcacgttt tgcgggtccc caggagcggg
     4381 ttgcagtggg tcacggggct ggattccgtc agcccgtagc cttccacgat ggtgctgccg
     4441 gtcagttcct cgaaccttcg caggacttcg gcgggcatgg gggaagcgcc ggtcacgcaa
     4501 agccggatgg aggaaaggtc gtagtcctgg accttgggat gattcacgat ggccacaaag
     4561 atggttggga cccccggaaa gaccgtcggc ctactcttgt ggagaatctt catgaactcg
     4621 ttgatctcga aacggggaat gatgatggcg gcacatcccg tgtacaaggt caggttcatg
     4681 cccaccgtca tcccgaacac gtgggaaaac ggcagaatgg caacgaagcg ttccttcccc
     4741 cgctcaatct tctggaacca tgcgatgatt tggttcacgt tggccaccag gttccggtgg
     4801 ctgagcatga ctcccttggc gatccccgtg gtccccccgg tatactgaag gaccgccaga
     4861 tcgtcgggtt ccaccgt
//
LOCUS       DAOOVC010000227         4855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9749_length_4855_cov_6.066875, whole genome shotgun sequence.
ACCESSION   DAOOVC010000227 DAOOVC010000000
VERSION     DAOOVC010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9749_length_4855_cov_6.066875"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2267
                     /locus_tag="PLM79_17305"
     CDS             <1..2267
                     /locus_tag="PLM79_17305"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV88116.1"
                     /translation="WTVEAIQGSVPRLLQWIEEDFGAFLRVLECRYISGNRQLLDQLE
                     KALDLKIEERRDELLARLHQEVKEREVRLEGVESWLEPDLEQDPGGLADINAIRLAGR
                     ISTNTRNLEDAIFRGYLTRQEVDLLQQAEKYYARLGSLIHDASGASRSVLRVERQDYV
                     AGRLGYSAGVGFLPVESFLQHVHRFLHGVQCISQEFWARVWESRQGDPDALSSSTLET
                     GLQVRSGKILVQTDRYPATPGNILRVFALSAVHRLELGNVTRQWIHHHENALSSCAGD
                     PRVKEGLLDLLRWDEVDLPAVRRFYNMGLAASLIPELNGVHGLVQHDSFHLYPVHEHH
                     LRTVMELKRLFRGDYAGEEEAITEIARGVTDPAWLYLAGLLHDMGKSAGRGHALRGGE
                     MIPAVSKRLGLTTEECELLQFLVAEHLLLMDNASLRDLADEEMLAQCALTVGNVENLD
                     LLLLLSFADMRATGPRAREKWNETPAVRLYERVRHLLEKGEPSPKAIAERMERVKARI
                     RREVSDLMDDQELDRYVSQLDPRYLLSVTPHAMAGHLRLQWRMQNSEEPFILEVEESD
                     NSAELTLLVRERPGLLFRAAGILTLHDMNITGAQVFTMNSRMSLLLFQCRLPQHSPQG
                     PDWNRVRADMKRLLSGKMSLDYRIAAHAAGRRHRPEGIRKAPSQILIDNESSALYTIL
                     EVYTVDRVGLLYTIARTLFELQVRIFVAKITTKVDQVADVFYVRTRQGEKVTDPDQTE
                     EIRKALLFWLDGDG"
     gene            2831..3478
                     /locus_tag="PLM79_17310"
     CDS             2831..3478
                     /locus_tag="PLM79_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88117.1"
                     /translation="MYEFVRGPLTWVAFIVFVGGMTYRIVSLILLSRKKDPVVYNHLS
                     LSWSLRSIFHWLLPFNRTVTQYPVYSFFAYVFHFCLLLLPIFLLAHNILWYESWQIRW
                     WSLPESLADYLTMVVLACIVFLIVRRTVLPYVRIVTTGMDYFLLIVVALPFLTGLIAY
                     HQWWEYRWMLILHMLSGQLMLVLIPFTKLSHMAFFFLTRAHIGSEFGERRGTVTW"
     gene            3539..4840
                     /locus_tag="PLM79_17315"
     CDS             3539..4840
                     /locus_tag="PLM79_17315"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOV88118.1"
                     /translation="MSETVNVEVIRQKLEAAKGARMKLWLEMCAHCGLCADSCFFYLA
                     HDKDPTYMPSYKVVQTLGEMYRRKGNVDRKFLEEAHDVVWGKCTMCRRCSMYCPFGID
                     MATMIAVARGVCNSQGVVPEGLQRAVNNYVESGNQMGMKLEDWVETLEWMATETAEEI
                     AGLEIPLDKEGANILYTVNAREPMYYPQDIAMAAKIFTLAGEDWTMSSDGWDDTNLAM
                     FAGDAKVMAQVAKNTYDAAIRLKAKHIMITECGHAYRSLRYEAPYWLGYPDGQPPVPV
                     IHSVEVFARYLQEGRIRIDPAHRIKEPVTYQDPCSHSRNGNLAEYGRYLVRQLADDFR
                     EMTPNREHNHCCGGGGGFIPMGPPFKKRRIQSGKVKAEQIRATGAKLVITPCHNCFDQ
                     IRDLSKEYDLGVSVKSFKELITEMMIIPEEFLPKEEDEGEE"
BASE COUNT         1010 a         1406 c         1485 g          954 t
ORIGIN      
        1 ggtggacggt ggaggcgatc caaggatcgg tgcctcgatt gctgcaatgg atcgaggaag
       61 acttcggagc gttcctgagg gttctggaat gtcggtatat ttccgggaat cgacagctac
      121 tggaccagtt ggaaaaggcc ctggacctca agatcgaaga aaggcgcgac gagcttctcg
      181 cccggctcca ccaagaagtg aaggagcggg aggtccgtct cgaaggggtg gaaagttggc
      241 tggaaccgga cctcgagcag gaccccgggg gccttgcgga catcaacgcc attcggttgg
      301 cgggccgcat ctccaccaac acccgaaacc tggaggacgc catcttccgg ggctacctca
      361 cccggcagga agtggacctg ctccagcagg cggaaaagta ttacgcccgc ctgggcagcc
      421 tgattcacga cgcgtctggg gcttcgaggt cggtcctgag ggtggagcgt caggattacg
      481 tagccggtcg attgggctac tccgcgggtg ttgggttcct gccggtggag agcttcctgc
      541 agcatgtgca tcggttcctc cacggcgtgc aatgcatctc ccaggagttc tgggcccggg
      601 tgtgggaaag ccgccaggga gatcccgacg ccctttccag cagcacgttg gaaacggggc
      661 tccaggtgag gtccgggaag atccttgtcc agaccgaccg ctatccggca actcccggca
      721 acatcctgcg ggtcttcgcc ctctccgccg tccatcggct ggaacttggc aacgtgaccc
      781 ggcagtggat tcatcatcac gagaatgccc tgagctcctg tgcgggggac ccccgggtaa
      841 aagaggggct cctcgacctg ttgaggtggg atgaggtgga tcttcccgcc gttcggcgct
      901 tctacaacat ggggctggcg gcctctctca tccccgagct caacggagtt cacgggctgg
      961 tgcagcacga ttcctttcac ctctaccccg tccacgagca ccacctgcgt acggtgatgg
     1021 aattgaaaag gctgttccga ggggattatg ccggggagga ggaggccatc accgaaattg
     1081 cgcggggcgt gaccgatccc gcgtggctct accttgccgg cctgctccac gatatgggaa
     1141 agagcgctgg gagggggcac gcccttcgcg gcggagagat gatccctgcc gtctccaagc
     1201 gactggggct gaccacggag gaatgcgagc ttcttcagtt cctcgtggcc gagcatctcc
     1261 tcctcatgga caacgcgtcc ctgcgggact tggccgacga ggaaatgctc gcccagtgcg
     1321 ctctcaccgt gggaaacgtg gaaaacctgg acctgctgct gctgctcagc tttgccgata
     1381 tgagggccac cgggccccga gcacgggaga agtggaacga gacgccggcg gtccggctct
     1441 acgaaagagt ccgtcatctg ctggagaaag gagagcccag ccccaaggcc atcgccgaaa
     1501 ggatggaacg ggtgaaggct cgaatccggc gggaagtgag cgatctcatg gacgaccagg
     1561 agctcgatcg ctatgtgtcc cagttggatc cccgttatct gctctcggtc acgccccacg
     1621 ccatggccgg gcacttgcgc ctccagtggc ggatgcagaa ttccgaagag cccttcatcc
     1681 tcgaagtcga agagtcggac aactcggcgg aattgaccct cctcgttcgg gaacgcccgg
     1741 gtctgctgtt tcgagcggcc ggaatcctca ccctccacga catgaacatc accggcgcgc
     1801 aggtgttcac catgaactcc cgcatgagcc tgctcctctt ccaatgccgc cttccccagc
     1861 actcccccca ggggcctgac tggaaccggg tgagggccga catgaaacgg cttttgagcg
     1921 ggaaaatgtc cctggactat cgaatcgcgg cccatgcggc cgggcgccgt catcggcccg
     1981 agggaatccg gaaagccccc agccagatcc tcatcgataa tgagtcctcg gccctctaca
     2041 ccatcctgga agtctacacc gtcgatcggg tgggcctcct ctacaccatt gccaggaccc
     2101 tgttcgagct gcaggtgcgc attttcgtcg ccaagatcac cactaaggtg gaccaggtgg
     2161 ccgacgtctt ttacgttcga acccggcagg gagagaaggt caccgatccg gatcagacgg
     2221 aagagatccg caaagccctg cttttttggc tggacgggga cgggtaggag agccggagga
     2281 ggtccccagg aggcgaggtt gtggaatttt gcacattgac gggggcgctt ttggggccgg
     2341 gaccgggaag cgggaggcgg ccgccgtcgg ggagccgtcc ccataaccgg atagaatttg
     2401 cataaaatgt ggagtctttc ctttgagaat ttggagatta tgtgttagaa agcccgtgag
     2461 agtttcactg aacaagtgaa tatttgaaca aaggaaaaaa gctgccgatt ccttggaacc
     2521 tgtcaccgta ggaccacctc ctgaggcgaa ttccccaatc ggaataggct ctctgcttga
     2581 cgacctcatg cgcagcccgc cggaggggcc gttgccgccg gtggaagcgg cgggaagcaa
     2641 cccggcacat cgtgaaagat cagaaggaat cggtttgtct ttgtgctcat ccggcgcgag
     2701 ccggtagccg ggtccggggg tctcccggcc ccaagttccc cggggcgact atgcacacat
     2761 gggtcccgcg gtccggggat gattccccgg gaacggacgg agatgaaacg cgagaaaagg
     2821 aggccaaagg atgtacgagt ttgtacgagg tccgctgacg tgggttgcct tcatcgtatt
     2881 tgtcggggga atgacctacc ggattgtttc cctgattctc ctcagccgaa agaaagaccc
     2941 ggtggtttac aaccatctga gtctgtcatg gtccttgcgc tccatcttcc attggctgct
     3001 tcccttcaat cgcacggtga cccagtaccc ggtttactcc ttcttcgcct atgtcttcca
     3061 tttttgcctc ttgctgcttc ccatcttcct gctggcccac aacattctct ggtatgaatc
     3121 ctggcagatc cgttggtgga gtcttcccga gtctcttgcc gactacctga ccatggtcgt
     3181 tttggcctgc attgttttcc tcatcgtccg ccggacggtt cttccctatg tacgaatcgt
     3241 caccacgggc atggattact ttctcttgat cgtggtggcc ctgccctttc tgacgggcct
     3301 gatcgcctac catcaatggt gggagtatcg atggatgctg atccttcaca tgctgagcgg
     3361 ccaactgatg ctggtgctga ttcccttcac caagctgtcg cacatggcgt tcttctttct
     3421 gacccgggcc catatcggca gtgaattcgg ggaaagaaga ggcaccgtca cctggtagcg
     3481 gggcgcaaca caagggattc gcgaaggaga aaccctgttt ttagaaggag caaaaaccgt
     3541 gtcggaaacc gtgaatgtgg aagtgattcg ccaaaaactg gaagcagcca agggtgcccg
     3601 catgaagttg tggctggaaa tgtgcgccca ctgcgggctc tgtgccgaca gctgtttctt
     3661 ttacctggcc cacgataagg acccgacgta tatgccttcc tacaaggtcg ttcagacctt
     3721 gggcgaaatg tatcgaagga aagggaacgt ggaccgcaag ttcctggaag aagcccacga
     3781 cgtggtgtgg ggaaaatgca ccatgtgtcg ccggtgttcc atgtattgtc ccttcggcat
     3841 cgacatggcc accatgattg ccgtggcccg gggggtgtgc aacagccagg gggtggtgcc
     3901 cgaaggcctc cagagggccg tgaacaatta cgtggaaagc ggcaaccaga tgggaatgaa
     3961 gctcgaggac tgggtcgaaa ccctggagtg gatggcgacg gaaaccgccg aagagatcgc
     4021 cggcctcgag attcccctgg acaaggaagg ggccaacatc ctctacacgg tgaacgccag
     4081 ggaacccatg tattaccccc aggacatcgc catggcggcc aagatcttca ccctggccgg
     4141 ggaggactgg accatgtcct cggacggctg ggacgatacg aacctcgcca tgtttgccgg
     4201 ggacgccaag gtgatggccc aggtggccaa gaacacctat gatgcggcca ttcgtctcaa
     4261 ggcaaagcac atcatgatca ccgaatgcgg tcatgcctac cgatccctgc gctacgaagc
     4321 tccttactgg ttgggatatc ccgacggcca acctcccgtg cccgtcattc actcggtgga
     4381 ggtgttcgct cgatacctgc aggaaggacg catccggatc gatccggccc accggatcaa
     4441 ggaacccgta acctaccagg atccctgcag ccattccaga aacggaaacc tggccgaata
     4501 cggacgttac ctcgttcgtc agttggccga cgatttccgc gagatgacgc ccaaccgcga
     4561 gcacaatcac tgctgcggcg ggggaggcgg attcatcccc atgggcccgc ccttcaagaa
     4621 gaggagaatc cagtccggca aggtgaaggc ggaacagatt cgagccaccg gggccaagct
     4681 ggtcatcact ccgtgccaca actgtttcga tcaaattcgt gatcttagca aagagtacga
     4741 cctgggagtc agcgtcaagt ctttcaaaga actcatcacc gagatgatga tcatccccga
     4801 agagttcctg cccaaggagg aggatgaagg ggaagagtaa gtcccgaggg agggg
//
LOCUS       DAOOVC010000228         4810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_9852_length_4810_cov_6.339222, whole genome shotgun sequence.
ACCESSION   DAOOVC010000228 DAOOVC010000000
VERSION     DAOOVC010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9852_length_4810_cov_6.339222"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..2260)
                     /locus_tag="PLM79_17320"
     CDS             complement(200..2260)
                     /locus_tag="PLM79_17320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOV88119.1"
                     /translation="MKDEDKSRSRLIAELESLRRQLAECEKERAECKEAEDALRESEM
                     RYRQIAENIREVFWMADIGLTRVIYVSPAYEEVWGRERESLYADPASWAETIHPQDRH
                     QVRRIMTSNPALGYSVEYRVVRPDGTIRWVSDRGFPICDPSGTVYRIAGIAEDITRRK
                     WAEEELDRYRDHLEDLVKERTATLGRVNEQLTREIEERRRAERALQESQQMLQSVLDT
                     IPVRVFWKDLDSVYLGCNRAFACDAGLQSPEEILGKNDFEMSWAEEAALFRSDDRLVM
                     ETGKPKLGYEELQTGPNGNKLWLRTNKVPLRGLDGEIKGVLGTYEDITAGKEAEEKLR
                     VASLYARGLIEASPDPLVTISLEGKITDVNRATEQVTGVSRRRLIGDDFSNYFTEPHK
                     AGEGYRKVLDEGWVRDYPLTIRHRSGKTTEVLYNATVYRNETGEIQGVFAVARDITRR
                     RQVERALEESSEKLKLFAYSVAHDLKSPAIGVYGLTKRLARHAGDAFDEKSRSYCDQI
                     LKLSEHIAALVDKINVYIATKETRPTIEAVKISEIFHMIEDEFSAQLSIREIQWIRPE
                     VPVEIKADRLGMVRAFRNFMDNSLKYGGERLRKIRIGYEESEDLHIFSFSDDGKGMAD
                     QDPEKIFKAFQRLGTSEGTLGAGLGLTIVKEIAEQHGGNVWLGPMDKKGITFYFSISK
                     SLGG"
     gene            complement(2511..>4810)
                     /locus_tag="PLM79_17325"
     CDS             complement(2511..>4810)
                     /locus_tag="PLM79_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88120.1"
                     /translation="ILWALASAPRATWACGPMLPRTVFTETNMPDAPVEEFLGGRLGI
                     LEPTYRRVYLVAAYRQLAGPPLDEKERAGLLSLVKSKMAAEASFDGPVVPEAEDPGMK
                     EWIQELRRVQRESDPAGASPGEDIRLTDPYLQGDFHFKNCLDDAFKTAAGTLRERTAR
                     FGAGSGEVASWIEAQNRVFANCSGKETIPSPPEPGIHPLIARDRTYQIASAHFYALNF
                     DKARELFEAVAKDEESPWRRRAPYLVGRVLLRKATLAAGVQGDSTGKDPEKARAEQRR
                     IRALLEDAEKQFSRVIEDPSLAETHSAARGLLQLVRFKLHPVQSRTELARGTLSPSPE
                     RDIQRTASDYTVLLDRFMKEKVGAESFANIGTVGSVEDEAKAVEDLLASLDGEHDLTD
                     WILTFQAGGDAALVRALKKYDEKGSLPWLMAVLSKIGPGHPRYASVAEAAALVPEDSP
                     GYATAAFHLARCTTEAGKAEEARSMLDSMLPLFEKRKTPRSAVNRLLGLRLASAKNLA
                     EFIRYAPRFAVRIEYSEAVPKDPLLDEDSTRILNLEMPLALLLETVQSPDLPENLGIA
                     LGRAVWVRALLLGNEAAALAAANFLKDRDGVLGPAMKGYLGAKDPGTRKYAGVLTILR
                     HPGLRPFVDHGNGRETFLDAIDDYRDNWWDHPGNKAAQEESRKGPEPFFPGPRQKAAA
                     AKEYKTLAALGSAPTYLSRFVASYGRENPSAPNLAEALHLAVKSTRFGLPDDQTGKYS
                     KEAFVLLHKKYPKSEWAKKTPYWFK"
BASE COUNT          861 a         1509 c         1401 g         1039 t
ORIGIN      
        1 gtgccggcct tcgtgggcat gacgagggga tggtctcgga atcgctcaaa tcaggaggaa
       61 tcaacctatt atcctcccaa gactctcacc cacccatccc aattcccggc tatcgaggat
      121 cggcaacgcc gtttgtccgg ccctccgggg cagcaggaaa agcgagatct tttgggaggc
      181 attcgggagc gttcctttct caacctccca ggctcttgga aatggaaaag tagaaggtga
      241 ttcccttttt gtccatgggt cccaaccata cgtttccgcc atgctgttcc gcgatctcct
      301 tgacgatggt gaggcccagg cccgccccga gggtcccttc ggaggtcccg agcctttgaa
      361 acgccttgaa gattttctcg ggatcctggt ccgccattcc cttgccgtcg tcgctgaaag
      421 aaaaaatatg aaggtcctcc gattcctcgt atccgatgcg gattttgcgg agtcgttccc
      481 cgccgtactt tagggagttg tccatgaagt tcctgaaagc tcgtaccata cccaaccgat
      541 ccgccttgat ttccacgggg acctcaggcc gaatccattg gatttctcga atgctcagtt
      601 gcgcggaaaa ctcgtcctcg atcatatgaa agatttccga aatcttgacg gcttcaatgg
      661 tcggacgggt ttccttggtc gcgatgtaaa cgttgatctt gtccaccagc gccgcgatat
      721 gttccgagag cttcaggatc tgatcgcaat aggatcgaga cttctcatca aaagcatccc
      781 ctgcatgccg ggccagtcgc tttgtcaggc cgtaaacccc gattgccggg cttttgaggt
      841 cgtgggccac ggaataggca aagagcttca gcttttccga gctttcctcc aacgctcgct
      901 cgacctgtct tcgccgggtg atgtcccgag ccacggcaaa gaccccctgg atttcccccg
      961 tctcgttccg gtaaacggtt gcgttgtaga gcacctcggt ggtcttgccc gacctgtggc
     1021 gaatggtgag gggatagtcc ctcacccagc cttcgtccag gacctttcgg tatccctccc
     1081 cggctttgtg gggttcggta aaataattgg aaaagtcgtc cccgatgaga cgcctgcgcg
     1141 atactcccgt cacctgctcc gtggcccggt ttacgtcggt gattttgccc tcgagactaa
     1201 tggtcaccag gggatcggga ctggcttcga tgaggcctcg ggcatagagg gaagcgactc
     1261 tgagcttctc ttccgcctcc ttgccggcag tgatgtcttc gtaggtgccc aaaacccctt
     1321 tgatctcgcc gtcgaggccc cggagaggaa ccttgttggt gcgcagccaa agtttattgc
     1381 cgttcggtcc cgtctggagt tcctcgtacc cgagtttcgg ttttccggtc tccatgacca
     1441 gacggtcatc ggagcgaaaa agcgcggctt cctccgccca gctcatctca aagtcgttct
     1501 tcccgaggat ctcctccggg gactgcagac ccgcatcaca ggcaaaggca cgattgcagc
     1561 ccaagtaaac cgaatcgaga tccttccaga agactcttac cgggatggta tccagaacgg
     1621 actgcagcat ctgctgggat tcctgcagcg cccgctccgc cctcctgcgc tcctcgattt
     1681 cgcgcgtgag ttgttcattg acccggccca gggtagcggt ccgttccttc accaggtcct
     1741 cgaggtgatc gcgatagcgg tccaattcct cttcggccca tttgcgccgg gtgatgtcct
     1801 cggcgatccc cgcaatccgg tagacggtcc ccgatggatc acaaatggga aagcctcgat
     1861 ccgagaccca tcgaatcgtt ccgtcgggtc gcacgacccg gtactcgacg ctgtatccca
     1921 gggccggatt cgaggtcatg atccggcgaa cctggtgccg gtcctggggg tgaattgttt
     1981 ccgcccagga agccgggtcg gcatacaggc tttcgcgctc gcgtccccac acctcttcat
     2041 aggcgggact gacatagatc accctggtca gaccgatgtc cgccatccaa aagacttcgc
     2101 ggatgttctc cgcgatctgg cggtaccgca tctcgctttc ccgcagggcg tcctctgcct
     2161 ccttgcattc cgccctttcc ttctcacatt cggccaactg ccgacgcagg gattccagtt
     2221 cagcgatcag ccggctcctg ctcttgtctt cgtctttcat ttggaccctc ccaacatgca
     2281 cgcacccgca tccaaggtcg attcgaagct gatgacacct gtggatctcg aagtctgtag
     2341 gtccagcggg catttgccgg aacgggtata agagggactg ccggttcttt gggcaacttt
     2401 ggaactcatt atggaggttc cggctaccgg attcaaggta aattcacggc cggttcaaac
     2461 cactccgccc gggtccggaa aggaagccgg tctcctgcag gaaggccggg ttacttgaac
     2521 cagtagggag tctttttggc ccattcgctc ttgggatatt tcttgtgcag gagaacgaag
     2581 gcctccttcg agtacttccc cgtctgatcg tccggaaggc cgaaacgggt ggacttcacc
     2641 gccaggtgga gagcttcggc caggttggga gccgatgggt tttccctgcc gtaagaggcg
     2701 acgaagcgac tgaggtaggt gggtgccgaa cccagggccg ccaaagtctt gtattccttt
     2761 gcggcggccg ctttctgccg ggggccgggg aagaagggct cgggcccctt gcggctttct
     2821 tcctgcgcgg ccttgttccc gggatgatcc caccagttgt cgcgataatc gtcgatggca
     2881 tccaggaacg tttcccgccc gtttccatga tccacgaaag gcctcaggcc gggatgtctc
     2941 aggatggtca gaacccctgc gtattttctt gtcccggggt cctttgcacc caggtacccc
     3001 ttcatggccg gacccaggac tccatcccgg tccttcagga aattcgccgc agccagagcc
     3061 gcagcttcgt tgcccaacag cagggcacgc acccagacgg cccgtcccag ggcgattccc
     3121 aggttttccg gcaaatccgg gctttgaacc gtctcgagaa gaagggccag gggcatctcc
     3181 aggttgagga ttcgggtcga gtcctcgtca agaagagggt cctttgggac ggcttcggaa
     3241 tactcgatgc gtaccgcaaa ccgcggagcg taccgaatga attcggcaag gtttttggcc
     3301 gatgccaaac gtagtcccag gagccggttc acggcggatc gtggagtctt ccgtttttcg
     3361 aaaaggggca gcatggaatc caacatggac cgggcttcct ccgcctttcc cgcctccgtc
     3421 gtgcatcggg ccaggtggaa agccgcggtg gcgtacccgg gagaatcctc gggaaccagg
     3481 gccgccgctt cggccacgga agcataccgg ggatgccccg ggccgatctt ggagagaacg
     3541 gccatgagcc agggaagaga acccttctca tcgtatttct tcagcgcacg gacaagggcc
     3601 gcatctccac ccgcctggaa ggtgagtatc cagtccgtga ggtcgtgttc cccgtcgagg
     3661 gaagccagga gatcttccac cgccttggcc tcatcctcca cggaaccaac ggtgccgatg
     3721 ttggcaaagg actccgcacc gaccttctct ttcatgaacc gatccaggag gaccgtgtaa
     3781 tcggaagcgg tccgttggat gtcccgttcc ggggaggggc tcagggtccc ccgggccagc
     3841 tccgttcggc tttggacggg gtggagtttg aatcgcacca gttgcagcag gccccgggcc
     3901 gcggaatggg tttccgccag ggacgggtcc tcgatcaccc tcgagaactg cttttcggca
     3961 tcctccagaa gggccctgat gcggcgttgc tcggcccggg ccttctcggg gtcttttccc
     4021 gttgaatccc cttgaacgcc tgccgccagg gtggcctttc ggagcagcac ccggcccacc
     4081 aggtagggtg cccgtcgccg ccacggggat tcctcatcct ttgccacggc ctcgaacagt
     4141 tcccgggcct tgtcgaaatt cagagcatag aaatgggccg acgcgatctg gtaggtcctg
     4201 tcccgggcga tgagggggtg gattcccggc tccggcggag aaggaatcgt ctcctttccc
     4261 gaacagttgg cgaaaacccg gttctgtgcc tcgatccagc tcgccacttc cccggaaccc
     4321 gcaccgaacc gggcggtccg ctccctcagg gtcccggccg cggtcttgaa ggcgtcgtcc
     4381 aggcagttct tgaagtggaa atccccctgg agatacgggt ccgtcaaccg gatgtcctcc
     4441 cccggggaag cccctgccgg atccgattcc ctttgcaccc gcctcagttc ctggatccac
     4501 tccttcatcc ccgggtcctc ggcctccgga acgaccggcc cgtcgaagga ggcctcggcg
     4561 gccatcttgc tcttcaccag gctcagcagt cccgctcgtt ccttttcgtc caggggcggg
     4621 ccggcaagct gccggtaagc ggccacgagg tacacgcgcc ggtaggtggg ttcgaggatt
     4681 cccagcctgc cgccgaggaa ctcctcgacg ggggcgtccg gcatgttggt ctcggtaaag
     4741 accgtgcgag gaagcatggg gccgcaggcc cacgtggccc ggggggcgct cgccaaagcc
     4801 catagaatca
//
LOCUS       DAOOVC010000229         4716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10080_length_4716_cov_6.937138, whole genome shotgun sequence.
ACCESSION   DAOOVC010000229 DAOOVC010000000
VERSION     DAOOVC010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10080_length_4716_cov_6.937138"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="selD"
                     /locus_tag="PLM79_17330"
     CDS             complement(<1..474)
                     /gene="selD"
                     /locus_tag="PLM79_17330"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(424..426),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOV88121.1"
                     /translation="MGVSRVELAKTVRAAGUAAKIGPGDLAQILEGLPLEEDPNLLVG
                     RETSDDAGVYRLTRDTALVQTIDFITPIVNDPYDFGRIAAANALSDVYAMGGRPLTVM
                     NVVCFPLKAMDKGVLKEILRGGIEKIHEAGAVLVGGHSVEDAEIKYGLSVTGVVHP"
     gene            complement(591..1196)
                     /gene="yedF"
                     /locus_tag="PLM79_17335"
     CDS             complement(591..1196)
                     /gene="yedF"
                     /locus_tag="PLM79_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HOV88122.1"
                     /translation="MEIELDCRGLACPQPVLKAKEVIEEGDADRVKIWVDNAAAKENV
                     SRFLSRMGYDVEISEKEGAYAVKAVRGREAAPCQMPLASAAEETRRRIAVLIGTDRMG
                     SGDEMLGEKLLLNFVGTLKEMGPELWRVILLNGGVRLSVEGSQSLPGLKELENSGVSV
                     LVCGTCLNHFGLLDRKRVGETTNMLDIVTALQVADKVISLT"
     gene            complement(1606..3828)
                     /locus_tag="PLM79_17340"
     CDS             complement(1606..3828)
                     /locus_tag="PLM79_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88123.1"
                     /translation="MGSILVVDDERSIRITLKAFLEGDGHQVVTAAEAQSAVACLGEA
                     PVDVVLTDIILPKVSGVELLRQIREMYPHVQVVMMTGEPTLETAYESLRLGAIDYLQK
                     PVGKTDILKTVRNALRVKELRDEKLRLEEENRNHMNRLEQLVEERTRALVESEAALRN
                     RAEELSVLNRLARRVNESISVEDVVKYGLGEIVSATGPDLAVFFLKEGEDLVPRGLLP
                     RGAESYWVPAGAHKVGTCLCGLAVSEDRAIFSTDVRSDVRCTMTECRQGGFASFAALP
                     LRSGSEIIGVLGIASVRPRDFRKNSRFLESLATEMTIGLKKSLLYEQVQQHAQELQAS
                     LTRTREAESERLMLQEHLQRAQKMEAIGTLASGIAHDFNNILGAVIGYAELALLESPK
                     DSHCRSRLEMVLAAGTRAKELVKQILAFSRQSEEELRPIDITHTVLEVLGFLRASLPA
                     TIEIRHQLDCDLGCVLADPVQIHQVVMNLCTNAQYAMSANGGVLEVQLSLLSLGHDHA
                     VVHPNLKPGRYVKLMVRDTGHGMDKAILEKIFDPYFTTKEKGVGTGLGLAVVHGIVQK
                     HGGVITVESELGKGSAFYVYLPAIERMKIPETPIREEIPGGREHILLIDDEQVLVDMG
                     KEMLELLGYSVETRTSSVEALALFSAQPHRFDLVITDMTMPNMTGDKLAMELLRVRPD
                     IPVVICTGYSEAFLEERIKDSGVSALVMKPVVCSELAKAVRKALNRKPDAGTDSQAGN
                     "
     gene            complement(3839..>4716)
                     /locus_tag="PLM79_17345"
     CDS             complement(3839..>4716)
                     /locus_tag="PLM79_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88124.1"
                     /translation="QSMQKTRDEGLNCKTSVNYFPIFDQQGRVEGVVLNARDITETRK
                     LEEQLMQSQRIESLGTLAGGVAHEINNPINGIMNYAQLILDGMGTESPAKEYAEEIVH
                     ETRRIASIVRNLLTFARHEKQSHSLALLSDIVASVFSLIRTVLRHDQIDLCVDLPDDL
                     PKIKCRSQQIQQVLMNLMTNARDALNERYPGYSPEKKIRVSAQIVQKGERRFIRTTVE
                     DTGIGIPPEIRDRIFDPFFTTKPKEIGTGLGLSISYGIVKDHGGELSLEAEPGRYTRF
                     HVDLPVENGWTLGGA"
BASE COUNT          877 a         1511 c         1312 g         1016 t
ORIGIN      
        1 gggatggacc actcccgtta ccgagagccc atacttgatt tccgcgtcct caacactgtg
       61 cccccccaca aggaccgccc ccgcttcgtg gattttctcg atccccccgc gaaggatctc
      121 tttcaacacc cccttgtcca tggccttcag gggaaaacag accacgttca tgaccgtaag
      181 gggccttccg cccatggcat agacgtcgct cagggcgttg gcggcggcaa tcctcccgaa
      241 gtcgtaggga tcgttcacaa tgggggtaat gaagtcgatg gtctgcacca gggccgtgtc
      301 ccgggtgaga cggtacaccc ctgcatcgtc ggaggtctct ctccccacca ggagattggg
      361 gtcttcttcc aacggcaatc cctcgagaat ttgcgccagg tcccccggac caatcttggc
      421 cgctcaaccg gcggcccgca cggtttttgc caactccacc cgcgaaacac ccatggcaaa
      481 cgtcctctct ttggccccgc aactccggga aagggctcca aacccggggc cgggcgacac
      541 cccgccgcct gggggcccga atcccgggaa acccccgaat cccgaagcga tcaggtcaga
      601 ctgatgacct tgtcggccac ctgcaaggca gtgacgatat ccagcatgtt ggtggtctct
      661 cccacccttt tgcggtccag cagcccgaaa tgattcaggc aggtgccgca aaccaggaca
      721 ctcactcccg aattctccaa ctctttgagc ccgggcagac tctgggaacc ttccacggag
      781 agcctgacgc ctccgttgag caggattacc cgccacagtt cgggtcccat ctccttgagg
      841 gtcccgacga aattcaagag gagcttctcc cccagcattt catcgccgga acccatgcga
      901 tccgtgccga tcaaaacggc aattcgtcgc cgggtttcct cggcggcgga ggcgaggggc
      961 atttggcaag gtgcggcctc ccttccccga accgccttca cggcataggc accctccttt
     1021 tcggagattt cgacatcata ccccatcctc gagaggaagc gactcacgtt ttccttggcg
     1081 gccgcattgt ccacccagat tttcacccga tcggcatcgc cctcttcgat cacttctttg
     1141 gctttcagca ccggctgggg acatgccagc ccccgacagt ccagctctat ttccactcgc
     1201 attctccttt gatcctcgtg tccaccgaat cttgccgccc cccggaaccc tcgcatccga
     1261 ccccgcgaca ccgacccgca atgaatcctc caatggagcg gaccccgtct gagggcttca
     1321 tcgctccctt tttcgcactc gggcggattt ccctcgtgaa gccgaatcct gcgcatcccc
     1381 atgagaaggt ttcggagcca atctttggag ggtcctctcc ccttcacggt ccatgaaatc
     1441 ccaagggggc gcttttttca aatcgacaat ttcgatggat tggcgttgga tcatcgcgac
     1501 ttccgatgca ggacgcacgg aagggggcgg gcggacgacc ttgagaggcg tggctgcgta
     1561 cgccgaaaat gggcggctcc ggggcggaaa caatccgggt cgaggtcagt tcccggcctg
     1621 cgaatccgtc ccggcatcgg gtttgcggtt cagcgccttc cgaacggcct tggccagttc
     1681 ggaacacacc acgggtttca tcaccagggc gctgacaccg ctatctttga tcctttcttc
     1741 cagaaacgcc tcgctgtagc cggtacaaat cacgacgggg atgtccggcc gaacccgcag
     1801 gagttccatg gccagcttgt cgccggtcat gttgggcatg gtcatgtccg tgatcaccag
     1861 gtcaaaccga tggggctgag cgctgaagag cgccagtgct tccacactgc tggtccgggt
     1921 ctcgacactg tagccaagca gttccaacat ttccttgccc atgtccacga ggacctgttc
     1981 gtcatcgatc aacaggatgt gttcacggcc cccgggaatc tcctcgcgga tcggcgtttc
     2041 cgggattttc attctttcga tggcagggag atacacgtag aacgccgagc cctttccgag
     2101 ctcactttcc acggtgatga cccccccgtg cttctgcaca atgccgtgga ccacggccag
     2161 gccaagaccc gtaccgaccc ctttctcctt ggtcgtgaag tacggatcga agatcttttc
     2221 caggatggcc ttgtccatcc catggccggt gtcgcgcacc atgagcttta cgtagcgtcc
     2281 gggtttgagg ttggggtgga caacggcgtg atcgtgcccc agtgacaaca aactcagttg
     2341 tacctccagg actccgccgt tggcgctcat ggcatactgg gcattggtgc acaggttcat
     2401 gaccacctgg tggatttgca ccggatcggc caaaacacat cccagatcgc aatccaactg
     2461 gtggcggatt tcgatggtgg ccggcaagga agcccggagg aacccgagca cctcgagaac
     2521 cgtgtgggtg atgtcgatgg gcctcagttc ctcctcgctc tgacggctga aggcgaggat
     2581 ttgcttgacc agctcctttg ccctggtgcc tgcggccagc accatttcga gccggctcct
     2641 gcagtgggaa tccttggggg attccaacaa cgccagttcg gcatacccga tcaccgctcc
     2701 caggatgttg ttgaaatcat gggcaatgcc gctggcaagg gtgccgatgg cctccatctt
     2761 ttgcgcccgc tggagatgct cctggagcat gaggcgctcg gattccgctt cccgagtgcg
     2821 ggtgaggctt gcctgaagct cctgggcatg ctgctgcacc tgctcgtaca acaggctctt
     2881 cttcaggccg atggtcatct ccgtggcaag ggactcgagg aagcgggaat tcttccggaa
     2941 gtctctcggc cggacggagg caatccccag gactccgatg atttccgaac cgctcctgag
     3001 cggcagggcg gcaaaggaag cgaacccccc ctgccgacac tccgtcatgg tacaccggac
     3061 atccgaccgg acatcggtgg agaagatggc ccggtcctca ctcactgcga gcccgcacag
     3121 acacgttccc actttgtggg ccccggcggg cacccagtag ctttccgccc ctcgagggag
     3181 aaggcccctg ggaacgaggt cctctccctc tttcaggaaa aagaccgcca aatcgggccc
     3241 cgtagcactc acgatctcac ccaacccgta tttcacgaca tcctcgacac tgatgctttc
     3301 gttcaccctt cgggccaggc ggttgaggac ggacagctct tcggcgcgat ttcgcagggc
     3361 ggcctcgctt tcgaccaggg cccgagtccg ctcttccacc agctgttcga gccgattcat
     3421 gtggttgcgg ttctcttcct ccagacgcag cttctcgtcg cggagttcct tgaccctgag
     3481 agcatttcga acggtcttga ggatgtcggt cttgcccacg ggtttctgga ggtagtctat
     3541 cgctcccagg cgcagggatt cataggcggt ctccagggtc ggttccccgg tcatcatgac
     3601 gacctggaca tggggataca tctcgcggat ttgccgtagc aactccacac cggatacttt
     3661 gggcaggatg atgtccgtga gtacgacatc cacgggcgcc tccccgaggc aagccacggc
     3721 cgactgagct tccgccgcgg tcaccacttg gtggccgtcc ccttccagga aggccttcag
     3781 ggtaatccgt atgctgcgtt catcatctac cacaaggatg ctgcccatcc gcacctcctc
     3841 aggccccgcc gagcgtccaa ccattctcta caggaagatc tacgtgaaaa cgagtatatc
     3901 gtccgggctc ggcctcgagg ctcaactccc cgccatggtc cttcacaatc ccgtagctga
     3961 tggagagccc caagccggtg ccgatttctt tgggtttcgt ggtgaaaaag ggatcgaaga
     4021 tcctgtcgcg gatttccggg gggatgccga tcccggtgtc ctcgaccgtt gtccggatga
     4081 accggcgctc ccccttttgg acgatctgcg ccgaaacccg gatttttttc tccgggctgt
     4141 atccgggata ccgttcgttc agggcgtccc gggcattggt catgaggttc atcaacacct
     4201 gttggatctg ctggctgcgg cacttgatct tgggcaaatc gtcgggaagg tcgacacaca
     4261 ggtcgatctg gtcgtggcga agaacggtcc ggatcaggga gaacacggaa gcgacgatgt
     4321 cggagagcaa ggcgaggctg tgggactgtt tttcgtgccg ggcaaaggtc aacaggtttc
     4381 gcacgatact ggcaatccgc cgggtttcgt gcacgatttc ctccgcatac tccttcgccg
     4441 gactctcggt acccatccca tccaggatca actgggcgta gttcatgatg ccgttgatgg
     4501 ggttgttgat ctcatgggcc acacccccgg ccagggttcc aagggactcg atcctctgcg
     4561 actgcatcag ttgctcttcc aactttcggg tttcggttat atcccgggcg ttgagaacga
     4621 ctccctccac tctcccctgt tgatcaaaaa tgggaaagta attgactgaa gttttgcagt
     4681 ttagcccctc gtcccttgtt ttttgcatgg attgaa
//
LOCUS       DAOOVC010000230         4671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10201_length_4671_cov_3.780546, whole genome shotgun sequence.
ACCESSION   DAOOVC010000230 DAOOVC010000000
VERSION     DAOOVC010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10201_length_4671_cov_3.780546"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLM79_17350"
     CDS             complement(<1..449)
                     /locus_tag="PLM79_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88125.1"
                     /translation="MFDPASISDHAPQPEKAIEKRNEENFLAKYDESFQADVLGMLVH
                     RHSFLKTNAPYLKTDFFANEIHRIFAGVVLDLHKAFPDEPVTWTAIINEFGKKKAGLG
                     IKTEDEHLYLEVLSQIKLPPANPAFIEREIQQFTSLKRMEDALTEAA"
     gene            complement(467..1786)
                     /locus_tag="PLM79_17355"
     CDS             complement(467..1786)
                     /locus_tag="PLM79_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88126.1"
                     /translation="MVERFLSESKAEWEDSVSALAGILIKMTEEYRNGMKLNVLHGDE
                     AENRLRTAAGPGLRQEDANFSHIVRASFLSEGEKGVRFKKEFLEKLAQVLKKLCSAGK
                     RGPLSIVCNPPNDRRADVRSKEDRLVEDVLTQGRNLYGFIDLKRMRKIADGRKTKKLF
                     LQEKSQSRQPTLNKHRKSRAQSFPDGLLPVAERVAELEKSPRYWGIEPKDRTRLFVQD
                     YRQYLLEDCTVGVNPQDSYVRATENWEVLESESDFYRHFHKARIVADLCGALYADYIE
                     AVGRWYEQGAGRHYGCEVAKPQLLYGDGARRRYYEWFDTERADELNLPTPLEGERLNA
                     ESYEGADDQIGYFTRCLKELHRVWSRLAQGKLTEEGLWLKIASAVENGLLPAAFAQSA
                     HNSILPRYDHELYLAFESDPDDGKWQRYDQSGLTFAEFLATTYRKAA"
     gene            complement(2275..3015)
                     /locus_tag="PLM79_17360"
     CDS             complement(2275..3015)
                     /locus_tag="PLM79_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88127.1"
                     /translation="MMNFQEDSEFGPWCERKVAFNFLLEKIDIDEKKACILPSDKEYL
                     PDNEKHFLPFMEVAIFRDSLGKCFYSVNFSSQTKLDKSRWKKIRKAVDSVLIGFNRDV
                     GEGQDLFLPDHLDEEYMEKQREIIDKRELIRDMAPLLRQRYKKLMGEIREVIRSGAKA
                     SDVLAKRYGLDKGKVEIELYGGHRAEPSKIALSALLKQLKDECVAEACGLDAESKLKD
                     IVNRKGEPYRGRKERNVIISKIPTISPK"
     gene            3336..4478
                     /locus_tag="PLM79_17365"
     CDS             3336..4478
                     /locus_tag="PLM79_17365"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOV88128.1"
                     /translation="MWRLLKMAYRNKETKKLSYIGEIEFQGERIRKKGFKTKGAAEEW
                     EVAELQRLKNPDLVPLPLPKPTTPMISFVSLAEKYLDYCKGRFGENTWKSKRFYYQVF
                     IGLYLKAPSVLAEDITKVQIIDYLSKIQSKEINPHEKINRKIDGNKTANRHLKELKAL
                     YEWAVENEVVKVNPVRRIEPYPESPFQKYVPSANDINQVLMAASRKEMDVITLLLHTG
                     GRAGEIRTLTWADVNFEKGWVQLWTKKRKGGKREADKLPMTPTMKEILQRLWDSHDKA
                     KAHVIINPETGEPYTRNSNLFRRMTSRLCKKVGVKEFTPHALRHHIASIINDSGKATP
                     SQSQKFLRHRRLTTTENYFHDLERSLCDLVDLIDRPIECDPQEEQA"
     gene            complement(4571..4664)
                     /locus_tag="PLM79_17370"
     tRNA            complement(4571..4664)
                     /locus_tag="PLM79_17370"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4627..4629),aa:Ser,seq:gct)
BASE COUNT         1103 a         1250 c         1186 g         1132 t
ORIGIN      
        1 taagccgctt cggtcaaggc atcctccatc cgtttgaggg aggtaaactg ttggatctct
       61 cgctctatga aggcggggtt agccggaggt aacttgatct gcgacaggac ttcaaggtag
      121 agatgctcgt cttcagtctt gatcccaagg cccgcctttt tcttgccgaa ctcgtttatg
      181 atggcggtcc aggtgacggg ctcatctgga aaggctttgt gaaggtcgag tacgactccg
      241 gcaaatatcc tgtggatctc gttggcaaaa aagtcagtct tcagataggg tgcgttggtt
      301 ttaagaaagc tgtggcggtg tacgagcatg cccagaacgt ccgcttgaaa ggactcatca
      361 tatttagcaa gaaagttctc ttcgttgcgc ttttcgattg ctttctcagg ctgtggtgca
      421 tgatcagaaa tcgatgctgg atcaaacatt ttggtagcct cctatgctat gccgctttgc
      481 ggtatgttgt tgccaagaat tctgcgaaag tgagccccga ctggtcgtac cgttgccatt
      541 ttccatcatc aggatcggac tcgaaggcca ggtatagctc gtgatcatat ctgggcaaga
      601 tgctgttgtg cgcggattgg gcaaatgcgg caggcaaaag cccgttttcc acggctgatg
      661 cgatctttag ccaaagcccc tcctcggtca atttgccctg agcgagccgt gaccagacgc
      721 ggtgcagttc tttgaggcac cgggtgaagt agccgatctg gtcatcagca ccttcgtaag
      781 actcagcgtt taaacgctca ccttcgagag gcgtcggcag gttgagttca tcagcccttt
      841 cggtgtcgaa ccattcatag tagcgcctgc gggctccgtc tccataaagc agttgaggtt
      901 tcgctacctc gcagccgtaa tgtcgccccg cgccttgctc ataccagcgc cctactgcct
      961 cgatgtaatc agcgtaaaga gcgccacata ggtctgccac gatcctggcc ttgtgaaaat
     1021 gccgataaaa atcggattcg ctctccaaga cttcccagtt ctcggttgcg cggacatagg
     1081 aatcctgtgg attcacgccg accgtgcagt cttcgagaag atactgccgg tagtcctgga
     1141 caaagaggcg agtgcggtct ttcggctcaa ttccccagta gcgaggactc ttttccagtt
     1201 cggcgaccct ctcggccacg ggaaggaggc cgtcggggaa agattgagcg cgagatttcc
     1261 gatgcttatt gagcgtcggc tggcgggact gggacttctc ttgcaggaag agctttttcg
     1321 tctttctgcc atcggcgatc ttgcgcatcc gcttcaggtc gataaacccg tacaggttac
     1381 ggccttgggt gaggacatct tcaacaagcc gatcctcctt gctgcgaacg tccgcgcgtc
     1441 tgtcgttcgg cgggttgcac acaatggaaa ggggacccct ttttcctgct gaacacagtt
     1501 tcttgagaac ttgggccagt ttctcaagaa actctttttt gaaacgtacc cccttctctc
     1561 cttcggagag aaaagaagca cgtacgatat gtgaaaaatt tgcatcctcc tgcctcaaac
     1621 ctggtcctgc tgcggttctt aggcgatttt cagcttcatc cccatgaagc acattcagct
     1681 tcatcccgtt gcggtattct tcggtcatct tgatcaaaat accggccaat gctgaaactg
     1741 agtcctccca ttccgctttg gattccgaca agaaccgttc caccacggga acaatcctgt
     1801 agctggtcct ccggccaggc gcagtcttct cgcgaacaag cttgtattcg actctgagct
     1861 ttcttagtgc tggaccgatg gagcgttccg tgatccctgt gctctcgtgg aggaggtttc
     1921 tgtcaatact tcggctcaag ctccgaagca ggacctccaa gaccgcctgt gctgcgttct
     1981 ttcgtgctgc cataccatct acccccatgt gctggcgagc ttgacgatcc tggctagacc
     2041 aaggtaaaat atatgtgtct tcggatcgcc aaacccattt tttggattca cggggagccc
     2101 gccagctccc cgttttttgt gccgcacaac gcgacccttt tattgtcgca aggctggcgc
     2161 ggcgaagctc aggaatgttt aagggtcaaa aagtttggtg gaaattccag aatcaggggt
     2221 aacccacgag ggaatcgtga tgggatttac ggggcttgta agtatctgag attattactt
     2281 cggggaaata gttggtatct tcgagataat cacattcctc tcctttcgtc ctcggtaagg
     2341 ttcccctttc cgatttacta tgtctttcag tttgctctcg gcgtccagac cgcaagcttc
     2401 tgccacacac tcatctttca actgtttcaa taatgcagag agagcaatct tggaaggctc
     2461 ggccctgtgc ccgccataca gctctatttc aacctttcct ttgtcgaggc catacctttt
     2521 agctaacaca tctgaagctt tagctccaga tcgtatcact tctcgaatct ctcccatcaa
     2581 cttcttgtag cgctgcctca gaagtggtgc catatcccga attagttctc gcttgtcgat
     2641 tatctcacgt tgtttttcca tatattcctc atcgaggtga tcgggtagga agaggtcctg
     2701 accctctcct acatcccggt taaacccgat gagaaccgaa tctaccgcct tcctgatttt
     2761 cttccatctt gatttatcga gtttggtttg agaactgaag ttgacgctgt agaagcactt
     2821 gcccaagctg tccctgaaga tcgcgacttc catgaacgga agaaagtgtt tttcgttatc
     2881 cggcagatac tccttgtcac tcggcaggat acatgccttt ttttcgtcaa tatcaatctt
     2941 ttcgagcagg aaattaaagg ccactttacg ttcgcaccaa gggccaaatt ctgagtcctc
     3001 ttgaaaattc atcattgctc ccatctgtca ctgaaaaact ggaaaattgg gccatcaaaa
     3061 ataacccatt ctttccagaa aatcagccct gaaaatcccg taacatcttc gtggggttga
     3121 gatcgacccc ttaagctgag tggttttgtc agagacattt cgtatcggac actaagcagg
     3181 accgttgttc aacatgcaca tcgttgactt ccacgaactc tcgtcgatcc tgaaggctaa
     3241 cccgaagacc ctgcaaaaga tttggaggga cctccctcac ttttttatcg ggactgggca
     3301 caacctgaaa ggggccagat ttgtcgtgga aaaagttgtg gaggctcttg aaaatggcgt
     3361 acagaaacaa ggaaacaaag aagctaagct acatcgggga aatcgagttc cagggggaga
     3421 gaatccggaa gaaaggtttc aagaccaaag gagctgccga agagtgggag gtcgccgaac
     3481 tgcaaaggct gaagaatcca gacctcgtgc cgctcccgct tccgaagccg acaactccaa
     3541 tgatctcctt cgtttcctta gctgagaaat acctcgatta ctgcaaggga cggttcggag
     3601 agaacacctg gaagtcgaag cggttttact atcaggtctt tatcggccta tacctcaagg
     3661 ccccaagcgt gttggctgaa gacatcacca aggtccagat catcgactat ctctcgaaga
     3721 tccagtccaa agagatcaat ccccatgaga agatcaaccg caagattgac ggcaacaaga
     3781 ccgcgaaccg gcacctaaag gaactgaagg ccctctacga gtgggcggtc gaaaacgagg
     3841 tggtgaaggt gaaccccgtt cgaaggatcg agccttaccc ggaatccccc ttccagaagt
     3901 acgtcccctc ggcgaacgac atcaaccagg tcctcatggc ggcatcgcga aaagagatgg
     3961 acgtgatcac actattgctc cacaccgggg gcagggcagg agagatccga accctcacgt
     4021 gggcggatgt caacttcgaa aagggatggg tacagctctg gacgaaaaaa cgcaagggcg
     4081 ggaagcgcga ggcagacaag ctcccgatga cgccgaccat gaaagagatc ctacagcgac
     4141 tgtgggatag ccatgacaag gccaaggcac acgtgatcat aaacccagag acaggcgagc
     4201 cgtacacccg caacagcaac cttttccggc ggatgacgag tcgcctttgc aagaaggtcg
     4261 gagtgaagga gttcaccccg catgcgctcc ggcaccacat cgcgagcatt ataaacgact
     4321 ccggcaaggc gacgccctct cagtctcaga agttcctgcg acaccggaga ctcaccacga
     4381 cggagaacta ctttcacgat ctggaacgga gtctctgcga tctcgtggac ttgatcgaca
     4441 ggccgattga atgtgatccc caggaggagc aggcgtgaaa aaagtccccc cgatttcccc
     4501 ccgatttttg gatttgggca aaacaaaacc cgaccgtccg ggggcggaca atcgggctaa
     4561 gtatctgttt tggcggagag agagggattc gaaccctcgg tagaactcta cgtcctacac
     4621 tcgcttagca ggcgagcgcc ttcagccgac tcggccatct ctccgaaact g
//
LOCUS       DAOOVC010000231         4652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10247_length_4652_cov_6.161845, whole genome shotgun sequence.
ACCESSION   DAOOVC010000231 DAOOVC010000000
VERSION     DAOOVC010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10247_length_4652_cov_6.161845"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PLM79_17375"
     CDS             <1..662
                     /locus_tag="PLM79_17375"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HOV88129.1"
                     /translation="TEAKTCPQSGAKVKRPMSKTKKILLGLFALLIVAGIFAPKDKDS
                     QQKTTQTAATTPAAAPVQSPGEKPENSNKSTAPAAEKPSKQIAGLGDEVNVGNFIYKV
                     NGVKFSKSVGNQFAQEKADGIFLLVDLTITNTSSETRLIDSALFKLQDQSNVSYEPSS
                     EGSTALELSGAKTLFLKQCQPNIPTKGVLVFEVPNQKESYTLNLSGGFWSAKNTHVKL
                     N"
     gene            complement(1392..1697)
                     /locus_tag="PLM79_17380"
     CDS             complement(1392..1697)
                     /locus_tag="PLM79_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88130.1"
                     /translation="MAPYPGTGTGQGDPRKCNQQAKTSAPVQEWKEIALGLVILRMFW
                     DLAGFGVNSWDWEILGVSKTQFVGGLGVRILRLKLRFGDQKWGGVFGVRIVYKLFAN"
     gene            2180..3637
                     /locus_tag="PLM79_17385"
     CDS             2180..3637
                     /locus_tag="PLM79_17385"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOV88131.1"
                     /translation="MVSVKDIRRISETVWEIPTSFKEGMRVPARIYATEKLLREMDDG
                     VVDQVTNVATLPGIVNHAYCMPDGHWGYGFPIGGVAAMDVRDGVISPGGIGFDVNCGM
                     RLVLTNLTLEEVQPRLRDLVDRLFARVPAGVGSTGFVRLTQDEFRTVVEQGARWCVDR
                     GYGWEEDLERTEENGCIEGADARKISDRAVERGYRQIGTLGSGNHYLEIQVARPEHIL
                     DPDLAKAFGITLPNQVAVMFHCGSRGFGHQVATDYLQLFLKVMERKYGIRILDRELAC
                     APFQSEEGRDYFAAMKCAVNMSFANRQVILHRVREAFSEIFHKDPKDLGLHQVYDVAH
                     NTAKIENHRVDGRLRQLLVHRKGATRAFGPGMEGLPPLYRTTGQPVILGGSMETGSYL
                     LVGVETGSQTFYSTAHGSGRTMSRRQAKRQFQGKKLQQDMEQRGIYLRTVSYAGLAEE
                     AGPAYKSIDDVVAATELAGISRRVVRFVPIGNVKG"
     gene            3642..4541
                     /locus_tag="PLM79_17390"
     CDS             3642..4541
                     /locus_tag="PLM79_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOV88132.1"
                     /translation="MSSKTPISLVTGSLGSGKTTLLREIIESFPGRLAVLMNEFGEIG
                     IDGRLLRGRNLEMVELTGGCVCCSLSGEFEAAVREILDKIEPDLIVVEATGVAEADAL
                     VFDIEDSLPEVRLDGILCIVDGYASVRYPSLGYTARTQLEVADVVLINKTDLIKPEEV
                     RRVEDQVRRFNGRAVLVPTVRCRVDLRVLLGIHSLGLPRRAPASQDQPAFRSFVYRAE
                     RPLDEAKFRQWASDLPLSVYRAKGFVRFASGGMLFNYVAGRWDLEVFAGDRTELVFIG
                     VGTDADRESILRRLEGCGIQDAL"
     gene            4531..>4652
                     /locus_tag="PLM79_17395"
     CDS             4531..>4652
                     /locus_tag="PLM79_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOV88133.1"
                     /translation="MPFRYIEDIATADVAFEAWGETLEDLCVAAADATMNVMVEV"
BASE COUNT         1069 a         1360 c         1334 g          889 t
ORIGIN      
        1 gcaccgaagc caaaacatgc ccgcaaagcg gtgcgaaggt caaacgaccg atgtccaaga
       61 caaagaaaat cctcctcggt ctatttgccc tgcttatagt tgcaggcata tttgccccca
      121 aggataaaga ctcacagcag aaaaccactc agacggcggc aacaacccca gccgccgccc
      181 cagttcaatc accgggcgaa aagccggaaa attcaaataa gtccaccgct cccgccgccg
      241 agaaaccatc aaagcagatt gccggcctgg gcgacgaggt gaatgttggt aacttcatct
      301 acaaggtgaa tggggttaag ttttcaaaat cagtcggtaa ccaattcgca caggaaaagg
      361 ctgatggcat atttctcctt gttgatctaa ccataacgaa tacatcctct gaaacccgat
      421 tgattgatag cgccctattc aagctccagg atcaatcaaa tgtttcatat gagccctcat
      481 ccgaaggctc cacggcactt gaactatctg gagccaagac actattcttg aagcaatgcc
      541 agcccaacat cccaaccaag ggtgtccttg tatttgaagt ccccaaccag aaggaatcct
      601 ataccttgaa cttgagtggt ggcttttggt ctgcaaaaaa cactcatgtc aaattgaact
      661 aacagcaggt tactaaagga ccaacaccca taactcgaag acggcttact ttcggccttg
      721 ctatgtcgct catatctgtc ctgtgtgcca ttactccgct ctatggctgg cagggaaggg
      781 tgatccaggt aattgatggc gataccatgg tggtcatgaa aggcgaagaa tccgtgaacg
      841 tccgactcta tggcatcgac tgccctgaag gcaatcaggc ttccggtcga gaagcttgga
      901 tctttgcttc aaagctcgcg aacggcagcc aagtggaagt ccagcccatc gacatcgacc
      961 gatacgggcg aacggttgcc actgtccacg tcaacggccg gtccataaac gaagaattga
     1021 tccgctccgg ccacgcctgg gtctacactc agtattgcac aaacgacttc tgccacgact
     1081 ggctgaaact ccaatccgac tcccagggcg cgagatccgg cttatgggca gccaaaaccc
     1141 caacaccacc atgggacttc agacactccc tcaacctcgc tcaggcaaca cctcaatccc
     1201 caaaacccaa gccacctccc gcaagcgccg ccccatacca cggcaacgta tcctccctca
     1261 tcttccacga ctcctcctgt caacactacg actgcaaagc ctgcacagcc gccttcacca
     1321 cccgccaaca agccctcgac gcaggcaggc tacagatcct gcagaaagtg cggcgcaaaa
     1381 tgacccccac ctcagtttgc aaacagttta taaacaattc gcaccccaaa caccccgccc
     1441 cacttttgat caccaaaacg caatttcaac cgcaaaatcc tcacccccaa cccacccacg
     1501 aattgcgttt tgctcacacc caaaatttcc caatcccacg aattcacacc aaatcccgcc
     1561 aaatcccaaa acatccgcaa aatcaccaaa cccaatgcaa tttccttcca ttcctgaacc
     1621 ggtgccgacg tcttcgcctg ctggttgcac ttacggggat ccccctgccc tgtgcccgtt
     1681 ccgggataag gtgccaaggg ttttggggag tcccgtggaa atcgaagatc cgagccgagc
     1741 tcggcgcttc actggggcaa aacggtgatc ggcgtcgcag caagtgcctc gggcatatcc
     1801 gttcgtaacg aaacctcacc ccgctgcgtg aggggaagat ccggaccggg agcgggacag
     1861 gacaaattca ggcgttgcag ggaagaaaaa ttgctggcca actggaagac atccttcacg
     1921 aatggcacta ccctaaagaa gcgctattgg tgtgtcaagg cggaattcgc gtggaattcc
     1981 ccaacgggct ccggtggagg gcgccggcaa gggaaaggac gatgggaagc ggttttgacc
     2041 ccggcatgtg attacattcc gggtcaagat gctatgatag aagggtcggt tttccgaaac
     2101 caacggacaa gacgaccgcg gtctcggaca aaccgtgggg gggatgcttg taaaaatacg
     2161 gcagaaaggg ggatgagcga tggtgtcagt caaggatatt cgcaggatct cggagacggt
     2221 gtgggagatt cccacgtcct tcaaggaggg gatgcgggtt ccggccagaa tctacgccac
     2281 ggagaagctc cttcgagaaa tggacgacgg ggtcgtggac caggtgacca acgtggccac
     2341 ccttccggga atcgtcaacc atgcctattg catgcccgac ggccattggg gctacggctt
     2401 tcccatcggg ggtgtggcgg ccatggatgt tcgggacggg gtcatttccc ccggcgggat
     2461 cgggttcgat gtgaattgcg gcatgcgcct ggtgctgacc aacctcactc tcgaggaagt
     2521 gcagccccgc ttgagagacc tggtggatcg gctctttgcc cgggttcccg ccggagtggg
     2581 cagcacgggc tttgtgagac tcacccagga cgagttccgc acggtggtcg aacagggggc
     2641 ccggtggtgt gtcgatcgag ggtacggctg ggaggaagac ctcgaacgaa ccgaggaaaa
     2701 cggctgcatc gagggggcgg atgcccggaa gatcagcgac agggccgtcg agcgggggta
     2761 caggcaaatc ggcaccctgg gatccggaaa ccactatctc gaaatccagg tggcccgtcc
     2821 cgaacacatc ctggaccccg atctggccaa agccttcggg atcacccttc ccaaccaggt
     2881 ggcggtgatg tttcactgcg gaagccgagg attcggtcac caggtggcga cggattatct
     2941 gcagcttttc ctcaaggtga tggaacgaaa atacggaatt cgcatcctgg accgggaact
     3001 ggcgtgcgct ccctttcaat ccgaggaagg ccgagactat ttcgcggcca tgaagtgcgc
     3061 cgtcaacatg tcctttgcca atcggcaggt gattcttcat cgggttcgcg aggccttttc
     3121 cgagatcttc cataaggatc ccaaagattt gggacttcac caggtctatg acgtggccca
     3181 caatacggca aagatcgaga atcaccgggt ggacggccgg ctaagacagc tcctggtgca
     3241 ccggaagggt gccacccggg ccttcgggcc cggcatggaa gggttgcctc ccctgtaccg
     3301 gacgacgggt cagcccgtca tcctgggcgg cagcatggag acgggaagct acctgctggt
     3361 cggggtcgag accggctccc aaaccttcta cagcacagcc cacggaagcg gacggaccat
     3421 gagccgccga caggccaaga gacagttcca gggaaagaaa cttcagcaag atatggaaca
     3481 gcggggcatc taccttcgca cggtctctta cgccgggctg gccgaggagg ccggtcccgc
     3541 ttacaagagc atcgacgatg tggtggcggc cacggaactg gcgggaatca gcaggcgggt
     3601 cgtccggttc gtccccatcg ggaacgtcaa ggggtgagga catgtcctcg aagacgccca
     3661 tttccctggt cacggggtcc ctgggaagcg gaaaaaccac tcttctccga gagatcatcg
     3721 agtcgttccc gggccgattg gccgtcctga tgaatgaatt cggcgagatc gggatcgacg
     3781 ggaggcttct ccgagggcgg aacctcgaaa tggtggagct taccggaggg tgtgtgtgct
     3841 gttccctgag cggagagttc gaggccgccg ttcgagagat cctggacaag atcgagcccg
     3901 acctcatcgt ggtcgaggcc acgggggtcg ccgaggccga cgccctggtg ttcgacatcg
     3961 aggacagtct gcccgaggtg cgcctggacg gcatcctttg catcgtggac ggatacgcct
     4021 cggttcgcta cccgtccttg ggatataccg ccaggaccca actggaggtt gccgacgtgg
     4081 tcctgatcaa taaaacggac ctaatcaagc ctgaagaagt ccggcgggtg gaggaccagg
     4141 ttcgtcgatt caacggccgg gccgtcctgg tcccaaccgt tcggtgccgc gtggatttac
     4201 gggttctcct gggcattcat tccctcggac tgccccggcg ggcccctgct tctcaggatc
     4261 agccggcgtt ccgttccttt gtgtaccgag cggagcgtcc cctggatgag gcgaagtttc
     4321 ggcaatgggc ttccgacctg ccgctttcgg tctaccgggc caaggggttt gtgcggtttg
     4381 cctccggcgg catgctgttc aactacgtgg caggtcgttg ggacctggaa gtctttgccg
     4441 gggacaggac cgagctggtg ttcatcgggg tgggaacgga tgccgatcgg gaatccatcc
     4501 tccggcgcct ggagggatgc gggattcaag atgcccttta gatacatcga ggatatcgcc
     4561 accgccgatg tggccttcga agcctgggga gaaaccctcg aggatttgtg cgttgcggcg
     4621 gcggacgcca ccatgaacgt catggtggag gt
//
LOCUS       DAOOVC010000232         4646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10259_length_4646_cov_4.264866, whole genome shotgun sequence.
ACCESSION   DAOOVC010000232 DAOOVC010000000
VERSION     DAOOVC010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10259_length_4646_cov_4.264866"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="PLM79_17400"
     CDS             complement(<1..1576)
                     /locus_tag="PLM79_17400"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOV88134.1"
                     /translation="MPRDDEYSNLISEIKNRADCRAIFRRFWPERYKEKGNCLCPFHE
                     DRDPSMQVTKDLAHCHAESLSLDAIDLFARGAACSPGDAIRLLASELGIERHGDAPGS
                     RLQPRSEYFSKRMQDALDCALPDEAVRYLNGRGISLETIQALKARKLIGWDRKLGGLA
                     FPLHDWSGREIVGIQVIPVNGEKKRFVKGTEPKRGFFRFGYGQELTVFCEGIMDALSV
                     AEATAKAEGVALMSAGLTEKLGNVDLPPAPVLFMDNDDAGRKALAKVMRVHGGKGRFR
                     VVDWQESLKGIGEDQSIKDVNDLLKSGHGEVIVKMIESSRPPMGDDLQRILSCNGSSR
                     LRAEHGGGQSAGDGDESKKQTQAQMLIMLAVGADFYHTPEQMIYASYTVNDHVETWHV
                     RSKNFRRWLSHRFYLEQGKPPGSQAIQDALNILEAQGQYDGPEFEVFTRVAGVADRIY
                     IDLANEAWQVIEITPHGWSVIENSPVKFRRTKGMLPLAQPEHGGSFEELRRFINLQDE
                     DTLKLVIGWLVQAGHPS"
     gene            complement(1806..2129)
                     /locus_tag="PLM79_17405"
     CDS             complement(1806..2129)
                     /locus_tag="PLM79_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88135.1"
                     /translation="MNMDCKHFLTCNANLCPLDPDIASRSWFIGEDVCKHKDHKDLPV
                     IRRQKQLNRLKPAKYAEKPLHASWLRKTAPRKISLSPEEKAERLARLHRKTSNDTYPP
                     AQGDS"
     gene            complement(2184..2513)
                     /locus_tag="PLM79_17410"
     CDS             complement(2184..2513)
                     /locus_tag="PLM79_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOV88136.1"
                     /translation="MLSRLEIARTVAEREKITIQDAYSIIGTTIDVIAESLEKDNVQL
                     TGLGTFKKVTLPAREGRNPKTGETIQIPERAVVRFKPSKRLLPSERQLRTSRGNGKKR
                     GRKSTGR"
     gene            3277..3855
                     /locus_tag="PLM79_17415"
     CDS             3277..3855
                     /locus_tag="PLM79_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88137.1"
                     /translation="MTASEKQYPGHYSTNDCFRAALAQLIEDEGHGAQVRLAKAIGRG
                     TKHLNDVIKGRRDAGIKFQESVAAYFKITHEEMIAMGRRFLSEKAGAFPYATEASKYE
                     NDEERGEFIYRKVLEEAGLSGNRFFSDRKVSDLLPSEWKGYIVGRSIDDFSLYSRTKE
                     ELQRFADLVIKNIPSKRRSRKKKLGLKDQPTE"
     gene            4216..4392
                     /locus_tag="PLM79_17420"
     CDS             4216..4392
                     /locus_tag="PLM79_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88138.1"
                     /translation="MAKRKKGKSLVDEACKWASSPEGMEEIEKALEEAKKLIDHLKQA
                     QLLDPRVLNEPITL"
BASE COUNT         1080 a         1178 c         1281 g         1107 t
ORIGIN      
        1 ccgagggatg acctgcctgt acaagccagc cgatgacgag ttttagggta tcttcatcct
       61 ggaggttgat gaagcgccta agctcttcaa aagaccctcc gtgctccggt tgggcaagcg
      121 gaagcatgcc ctttgttctt ctaaatttca caggagagtt ttcaatgacg ctccacccgt
      181 gaggcgtgat ctcgattacc tgccaggctt cattggccag atcgatgtag atcctatctg
      241 ccacgccggc aacgcgcgtg aaaacctcga actccggccc atcatattga ccctgcgctt
      301 caaggatgtt cagggcatcc tggatggcct ggctccccgg aggctttcct tgttccagat
      361 agaacctgtg tgaaagccag cgccggaagt tcttggatcg aacatgccag gtttccacat
      421 gatcgttcac cgtataggag gcatagatca tctgctcggg cgtatggtag aaatcggcgc
      481 cgacagcgag catgatgagc atctgggcct gcgtctgttt cttgctctca tcgccatccc
      541 ctgcgctctg gccgccgcca tgctctgctc gaaggcggct gcttccattg caggatagaa
      601 tcctttgaag atcgtctccc atcggcggcc gcgagctttc gatcattttg acgatgactt
      661 caccatgccc gcttttgaga agatcgttga catcttttat gctttggtct tctccaattc
      721 ccttgaggga ctcctgccag tccacaaccc tgaaccggcc tttgccgccg tgaacgcgca
      781 ttaccttcgc aagcgctttc ctccccgcat cgtcattgtc catgaataga acgggagccg
      841 gcggaaggtc gacgtttccg agcttttcag tgagcccggc agacatgagg gccacccctt
      901 ctgcctttgc cgtcgcttca gcaacactca aggcatccat gataccctcg cagaagacag
      961 tgagctcctg tccatatccg aacctgaaga agcctcgctt gggctccgtg ccttttacga
     1021 agcgcttctt ttctccgttt acagggataa cctgtattcc gactatttcc ctgccgctcc
     1081 agtcatgcaa tggaaaggca agaccgccga gctttctgtc ccagccaatg agcttgcgcg
     1141 ccttgagggc ctgaatggtt tccaaactga tgccgcggcc gttgaggtag cggacagcct
     1201 catcggggag agcacagtca agtgcatcct gcattctttt tgagaaatac tcggagcgcg
     1261 gctgcaatct tgatcccggc gcatcgccgt ggcgctcgat tccaagctcc gatgccagaa
     1321 gacgaatagc gtcacccggg gagcacgcgg cgccgcgtgc gaagagatcg atcgcgtcca
     1381 gagacaatga ttcagcatga cagtgggcta gatcctttgt gacctgcatt gaagggtccc
     1441 ggtcttcatg gaaaggacac aggcagttgc ctttctcttt gtacctctcc ggccagaagc
     1501 gcctgaagat ggctcgacaa tccgccctgt tcttgatctc ggaaatgagg ttgctgtatt
     1561 cgtcatctct tggcatatcg agctatcctt ttgagattgc gggactttcc atgagtcgcc
     1621 tgactcgtgc tgcagcgcgg aatccggtat tttacgaagg atcatccgca gccttcggga
     1681 tgaacttttt cattccgccc ggtccccttg gaatggctcc tcctggagac gagtgcactc
     1741 caggcagggg agtttttgag cttccgccct ccggaagggt aaaaccatgg ggcaaggtca
     1801 tgggctcaag aatctccctg tgcagggggg taagtgtcgt tcgaggtctt ccgatggaga
     1861 cgggccagac gttcggcctt ttcttccgga gatagagaga tctttcttgg tgcggttttg
     1921 cgaagccagc tggcatgaag gggtttttca gcgtacttgg cgggcttcag ccgattgagc
     1981 tgcttctgcc ggcggatcac gggaaggtct ttgtgatcct tgtgcttgca gacatcctcc
     2041 ccgatgaacc acgagcggct ggcaatgtcc gggtcgagcg gacacagatt ggcgttgcag
     2101 gtcaggaagt gcttgcagtc catgttcatg ctggttgcac cttgcactgt atcgatctat
     2161 tatggaaaag cgcttcctca ttcctaccgg cccgttgatt ttcttcctct cttctttcca
     2221 ttgcctctcg aggttctcag ctggcgctcc gatggaagca ggcgtttcga tggcttgaat
     2281 ctcaccactg cccgttcagg gatctgtatg gtctcaccgg ttttgggatt tcggccctct
     2341 cttgccggca gtgtgacctt cttgaaggtc ccaaggcctg taagctggac attgtctttc
     2401 tcaaggcttt ctgcaatgac atcaatggtg gttccaatga tggagtaggc atcctggatg
     2461 gtgatttttt ctctttctgc tactgtccgc gcgatttcaa gtcttgataa catcgtcttc
     2521 tctccctggt tccccgattc tgatctcggc acaccgaagc tttgtagtga aattcattca
     2581 gggcatggct gtttccgagc gaccaagtac gtaatggctg atgaatggcc cttcgcggtc
     2641 ccttcatcac agtggtggct gggagcgcaa agctacgcca aactcggggc aggcttatct
     2701 ttcgctgtgc ccaagtagtg gaaaatatgt agctttatgg taagcgggat cgaatggcgg
     2761 ggttgttgaa gccaagctca agaagagttt tgaggctgag gattttgaga cggccgaaag
     2821 aattcggacc tgcaacaggc gcaaatggta cagtgtcagc aacggctccc cttgactttg
     2881 actgccatgc tccaaaaatc atgcgcacca attgaccccg gaataccctc gttctttgtg
     2941 gaacgtttcc tctaccccaa ctccgctgag atcgagggcg aaatgccttg cattcggaac
     3001 gtgatgaagt caataacaca ggtgaatggt ctaaagcaag cacaattatc aattaaataa
     3061 tggagcaagt aagctcatga ttcgtgactt ttttctgctc catttagtat tgacaataat
     3121 agagcaggtt gctataaaat gttcacataa tgtagaaaga tctaggtctt gcgagagaag
     3181 gaggaaacgc tggcaggcaa tggatttctc ggcaggcagg aactttggca gatgcaaagg
     3241 gcctgctgag atggcgcaat gggaggagtg gagcaaatga cggcatccga gaaacaatac
     3301 cctggtcact actcgacaaa cgattgcttc cgggcagccc tggcccagtt gatcgaagat
     3361 gaggggcatg gagcacaggt gcgcttggcc aaggctatcg gaagaggcac caaacatctc
     3421 aacgacgtga ttaaagggcg cagggatgcc ggcatcaaat tccaggagag tgtggccgcc
     3481 tacttcaaga tcacgcacga agagatgatc gccatgggaa gacggtttct gagcgaaaag
     3541 gcaggcgcat tcccctacgc cacggaggca agcaaatacg agaacgatga agagagaggc
     3601 gagttcatct atagaaaggt gctcgaggag gcggggctct caggaaaccg tttcttttcc
     3661 gaccgaaaag tgagtgattt actgccttcc gagtggaagg gctatatcgt cgggaggagc
     3721 atcgatgact ttagcctcta cagccggacc aaggaagagc ttcagcgttt tgccgatctc
     3781 gtaatcaaga acatcccatc caaacgacgc agcaggaaga agaagctggg gttgaaagac
     3841 cagccgactg aatgacaaac catgaccaaa ggtattgact tttgggagga tttgcattta
     3901 ttgtgcccgt tattctcggg ataccccgtt cccgaaagaa ctcgccacaa gcgggtactt
     3961 ttttcttttc gctcttcttc gctaccagcg aagtgccttg gctcgatgat ggcatgaaga
     4021 ttccctgtaa tgtcagaaag gatttatccc gaattaagag gaggtgatgt agccggagta
     4081 tttgcatgag gatgcctccg tcacccgtga atcctgcgcg caagagaggg gctggcagta
     4141 gaaatgcgta gaaacaagga ttccgacagg ttagggggcc gagttggttt gattcgatct
     4201 gtaagggaga aaatgatggc taagaggaag aaaggaaaga gccttgtcga tgaagcttgc
     4261 aagtgggcct catcgccgga aggcatggag gaaatagaga aagcgctgga ggaggcaaaa
     4321 aagcttattg atcatctcaa gcaggcccag cttctggacc ccagggtttt gaatgaaccc
     4381 ataacactat aggagaaccg aactcgcaac tggattgggg gggagctgtg aacatcatag
     4441 acaagaagag aacaacaagg ccatacaaag tcataagctc tgcggtagca tctatcagat
     4501 gcggttgagt cccggacaag ggtgatcgtc ctccaaaagg acgtagagca taccgttgtc
     4561 gatggaggcc ggttggcctc aaaagagaag gggactaact caaccagggg taataaagca
     4621 tgccacgcaa gcgcaaagcc gcagag
//
LOCUS       DAOOVC010000233         4627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10308_length_4627_cov_3.402887, whole genome shotgun sequence.
ACCESSION   DAOOVC010000233 DAOOVC010000000
VERSION     DAOOVC010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10308_length_4627_cov_3.402887"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..528)
                     /locus_tag="PLM79_17425"
     CDS             complement(16..528)
                     /locus_tag="PLM79_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88139.1"
                     /translation="MRSFMSFPRTECDCSQCSISCKFIPGYLLPEDLIRMGRYLGHFF
                     LSPFIEENFLASPGALVAKAGRLYRIRTIVPARGENGWCKFFDGKLCTIHPVAPFGCA
                     FFDSHQDPILSGNLSTLGLMIIAAQWQNEESSNYCQAWHHLHQLNLRAPSPEECRQRM
                     QAATQLTVPL"
     gene            complement(883..1347)
                     /gene="ssb"
                     /locus_tag="PLM79_17430"
     CDS             complement(883..1347)
                     /gene="ssb"
                     /locus_tag="PLM79_17430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOV88140.1"
                     /translation="MLNQVCIAGNLGQDVELRYTQQGLAIANLNIAVNEHFKDSNGES
                     QKRTHWFRATAFGRTAEVAAQYLHKGSKVGIAGKLVQRTWQDDQGNNRSSVEIRIDQL
                     ELLGSANGSQNDGRQSTGQQPGGTAQNQSRPPQQQYYGDAYEPPPDSGDIPF"
     gene            complement(1362..1544)
                     /locus_tag="PLM79_17435"
     CDS             complement(1362..1544)
                     /locus_tag="PLM79_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88141.1"
                     /translation="MLVCKKVSKDFQSWTVSHGVTAELADGEDYQEALRTLRKQLVHL
                     VNQGLNGKNNTSLITP"
     gene            complement(1728..2267)
                     /locus_tag="PLM79_17440"
     CDS             complement(1728..2267)
                     /locus_tag="PLM79_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88142.1"
                     /translation="MWTKIRTKAKVILSILAVYISVTGLVTFSLFILEESIQLCVFGS
                     WPAQDAKDWQTVHLAYRRIIDTNKLLKTINYSIGWIQPLAFLGYRSYGHSTDIYIKGL
                     EAKIFANAPELFDGENLTVSFIPQEVRETADGVLHINHRVSFLSKKRHPAGVSCRITG
                     IVSAQGNKVVMRETEDQEP"
     gene            complement(2275..2601)
                     /locus_tag="PLM79_17445"
     CDS             complement(2275..2601)
                     /locus_tag="PLM79_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88143.1"
                     /translation="MKKIAIVALFVALVFPALAGAEDMILQAKASKVITDRLDKNGNP
                     FAIIFIQEERTLSGVAYTAEAPVFAMGPAKEEAGKVASGESFKAIVSKRQVNGDVTYT
                     LRKIIK"
     gene            complement(2977..3420)
                     /gene="rnhA"
                     /locus_tag="PLM79_17450"
     CDS             complement(2977..3420)
                     /gene="rnhA"
                     /locus_tag="PLM79_17450"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOV88144.1"
                     /translation="MKTVDIFIDGACSGNPGPGGWGAILCYGGHSREISGSESRTTNN
                     RMEMRAAIASLLMIKETCIVHVHTDSKYLQEGMTQYIHKWQENGWRNSNGQPVKNQDL
                     WEALLEACRIHQVTWHWVQGHNGNVRNERADCLARMAIQKANRSV"
     gene            complement(3611..4282)
                     /locus_tag="PLM79_17455"
     CDS             complement(3611..4282)
                     /locus_tag="PLM79_17455"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOV88145.1"
                     /translation="MKLVAVVGSRKLPSSWAGKVSQVVSDLVRRGCRIGSGGALGADH
                     FALQAAVNLGREACEGSKVFLPGSNLQAPPACRELLRRFESLGGSIVPGSAAGRATTR
                     SEYIGALFNRSVELVKVSSGIVAFIAGESKGTWFTCEQAARLGKQIILFSADGLRSLH
                     SLGCGSWVPVGCWQGAWRWETSVPAGKKCRHGIVVECCGVGLYRGKGSGKEEMSHEDP
                     SDASA"
BASE COUNT          958 a         1268 c         1326 g         1075 t
ORIGIN      
        1 agacggaagc tctatttaca ggggcacagt caactgcgtg gcagcctgca ttctttgcct
       61 gcactcctcc ggagacgggg ccctgagatt cagctgatgc aggtgatgcc atgcctggca
      121 gtagttggag ctctcttcgt tttgccactg tgctgcgatg atcatgaggc cgagcgttga
      181 gagatttccg ctgaggatag gatcctggtg gctgtcgaag aaagcgcagc cgaaaggggc
      241 gacaggatgg atcgtgcaga gctttccatc gaagaacttg caccacccgt tctctcccct
      301 ggctggaacg atggtcctga ttctgtagag ccggccagct ttggctacca gagctcccgg
      361 agatgcgaga aagttctcct cgatgaaggg cgagaggaaa aaatggccga ggtatcgccc
      421 catccggatc aagtcttccg gaagcaggta gcctggaatg aacttgcagc tgattgaaca
      481 ctggctgcag tcgcattctg ttcgaggaaa gctcatgaag ctacgcatgg aatcctcctt
      541 gctcggagca gatggacggc gcttagggct tattgaaagg acgttcttcg tgaggcattt
      601 gatgggacaa acgctgcaat tccttttgat ggcgtggtgg ctgcgcgggg cgtgggaatg
      661 ttgccacccc accacagcgg cacggggctt gcgtgggccg gcagccggac tgctaccggg
      721 cattggatgc gccaaagggt ttcagactgc cggggactgc tgccggcccg caatggcaag
      781 ccgcgtgccg cccaccggat ggctgcggag tcctggagcg gcacatcatg gaagagcccc
      841 aggactcgca ggaatgctgt ataggcacag gaagtacccg acttaaaacg gaatatcgcc
      901 tgaatcggga ggcggctcat aggcatcgcc gtaatactgc tgctgtggcg gtctgctttg
      961 attctgagcc gttccgcccg gctgctgccc ggtgctctgc cgcccatcgt tctggcttcc
     1021 gttggcactg ccgagcagtt cgagttgatc gatgcggatt tcaacggagg agcggttgtt
     1081 gccttgatcg tcctgccagg tccgctgcac gagcttgccg gcgatcccga ccttggagcc
     1141 tttgtggagg tactgggctg cgacctccgc cgtacgaccg aatgcagtgg cccggaacca
     1201 gtgggtacgc ttctgggact cgccgttgct gtccttgaag tgttcgttta cggcgatgtt
     1261 caagtttgcg atggctaggc cctgctgggt gtagcgcagt tccacgtcct ggccgagatt
     1321 tcctgcgatg catacctgat tgagcatgat gtcctccttg gttacggtgt aatgagtgag
     1381 gtgttgttct tgccgttcag cccctggttc accaggtgca cgagctgctt tctcagtgtt
     1441 ctcaatgcct cctggtaatc ctcaccgtct gcaagctccg ccgtgacgcc gtgagatacc
     1501 gtccatgact gaaaatcctt cgagactttt ttgcagacaa gcacagtgac ttctcttacc
     1561 ttcattgcct cgcctccctt cgtatgtgtg atgagtgacc aatcaagttc actgaaggca
     1621 ggccttgatc caatttgaag tgggaagggc tttgggattg cagcaccagg tggtgaagca
     1681 gggaagggcc tgctgccttg ccccctggta ctgcgggaga ggattgttca tggctcttgg
     1741 tcttcggttt ccctcatgac gaccttgttg ccctgggccg agacaatccc tgtgatccgg
     1801 catgaaacac cggctggatg cctctttttc gagagaaaag agacacggtg attgatgtgc
     1861 aggaccccat cggctgtctc gcggacttcc tggggaatga aggaaacggt gagattctct
     1921 ccatcgaaga gctccggagc attggcgaag atctttgcct cgagcccttt gatgtagata
     1981 tctgtcgaat ggccgtagga tctgtagccg aggaaagcga gaggctgtat ccagccgatg
     2041 gagtagttga tggttttgag gagcttgttg gtatcgatga tgcgcctgta ggccaggtga
     2101 acggtctgcc agtcctttgc atcctgggcc ggccaactgc cgaagacgca taattggata
     2161 ctctcctcga ggatgaacaa cgagaacgtg accaggccgg tgactgagat gtacacggcc
     2221 agaatcgaga ggatgacctt ggctttggtt ctgatcttgg tccacatggc cgggctactt
     2281 tatgatcttg cgcagcgtgt aggtcacatc cccgttcacc tgacgcttag agacgatcgc
     2341 cttgaagctt tcgcccgaag cgactttgcc ggcttcctcc tttgccggtc ccatggcaaa
     2401 aacaggtgct tcagccgtgt aggcaacacc ggagagggtt cgttcttcct ggatgaagat
     2461 gatggcaaac ggattgccgt tcttgtccag tctgtccgtt atgaccttcg aggctttcgc
     2521 ctggaggatc atgtcttcgg cgccggcaag agcgggaaac acgagagcaa caaacagggc
     2581 cacgatagcg atcttcttca tgtggatctc ctttgaggtt tgagatgttt gatggttggg
     2641 ttgagttgtc cttctggatc atttgcatct attgctgtgt gtccgaatgc atcccacctc
     2701 ccttccattc acgaccggca aactgaaatc ccatcatgct ctttcaagct tttcgctcgt
     2761 ttggagctgc atttcctctc tggctgccct ggcaaggtcc atccgagcta ccaatgggtg
     2821 ccctgctcgt ggcggcgggg agcgctaccc agctaacctc tagccgcccg ctcggcagct
     2881 ggggagagaa gacacaaccc gaatgatgag tgatgaatgc ggaatacaat cacacgtcat
     2941 caattcatca ttcaaaaaag gcagacagaa accccatcag acggagcgat ttgctttttg
     3001 aatcgccatt ctggccaggc agtccgcccg ttcatttcgg acatttccgt tgtgcccctg
     3061 aacccaatgc caggtgacct gatgaatccg gcatgcctcg aggagggctt cccagaggtc
     3121 ctggttcttg acaggctgcc cgttgctgtt gcgccagcca ttttcctgcc atttgtggat
     3181 gtactgcgtc attccttcct ggaggtattt ggaatcggtg tggacatgga cgatgcaggt
     3241 ttccttgatc atgagaagag aggcgattgc agctcgcatc tccatgcggt tgtttgttgt
     3301 tcttgattca gatcctgaga tttctctgct gtgcccacca tagcacagga tggctcccca
     3361 tccaccgggg cccggattgc cggagcacgc tccatcaatg aatatgtcta ctgtcttcat
     3421 gggctacctc atttctattg gtgaacaggc gagccgggaa aggcgaagga agactcacct
     3481 gatcacccaa gtaccggtct atgcatagat gacgcggtag agaaagcagc gctcccacgc
     3541 tttcgaaacg gctattgcca gccgcttgcc caggagagga cgcaggcgac aaacatgcaa
     3601 ggaatttgct ctatgctgaa gcatcggacg gatcttcatg gctcatctcc tcctttccag
     3661 aaccttttcc cctgtagagt ccaacgccgc agcactcgac cacgatgccg tgcctgcact
     3721 tctttcctgc aggcaccgaa gtctcccacc gccaagcccc ctgccagcaa ccgacaggga
     3781 cccaactgcc gcacccgagg gaatggagcg agcgcaaccc atcggcgctg aaaagaatga
     3841 tctgctttcc gagccttgcg gcctgctcac aggtgaacca agtccccttg ctctcaccgg
     3901 caatgaaagc aacgataccg gaagagactt tgacgagctc aacggagcgg ttgaacagcg
     3961 caccgatgta ttcacttctg gtggtggctc tcccggcagc cgagcccggc acaatggatc
     4021 cgcccaggga ttcaaaacgc cgaagcagct cacggcatgc cggaggagcc tgcaagtttg
     4081 atcccggcaa gaagaccttt gatccctcgc aggcctcacg accgaggttg actgcggcct
     4141 gcaaagcaaa atggtcagca cccagggcac ctcccgaacc aatgcggcag ccgcggcgaa
     4201 caagatcgga gacaacttgc gagaccttgc cggcccagga tgagggaagc tttcttgaac
     4261 caacaacggc aaccagtttc atggctactc ctcctgaata aagggacagt cacgaacaca
     4321 tcaccaaaga ccaggaataa agcctgccct ggataggctg aaatcagatc tttagtgccg
     4381 gggattttgt gtcagcgtgg tgattgggtg gactaagaaa agcctgcttg agtgtatcta
     4441 cattggtatc atatggttct ggtggttctg gcgaagaatc gacctacacg gcgtcttgga
     4501 cttcctccga ttccggatca agcgtgtagc cctcttccgg gccatactgg atttcatcat
     4561 cggcattttg ggcggctccg acactctggg caacttcccg ggatgcgcct gtcgagtttc
     4621 ctggcgg
//
LOCUS       DAOOVC010000234         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10380_length_4601_cov_12.470524, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000234 DAOOVC010000000
VERSION     DAOOVC010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10380_length_4601_cov_12.470524"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLM79_17460"
     CDS             <1..685
                     /locus_tag="PLM79_17460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HOV88146.1"
                     /translation="MLDASGLGGSYYYFTQSAHESAVVQWKRHPNDNRPGPADYSPID
                     IEAEGFEFSGQGQLPRPKFRISNVTLALMSAVIAWNDLLGAVVTRYRTLKKYLYGQPG
                     YDPDAHFPEDVYVIERKTLQSKVMIEWELSSILDFQGQQLPRRKALRDYCTHRYRVWD
                     PNAEEFIYTHARCPYRGNRYYDALDVEQAGHPELDRCGKRLSSCRLRFTGKQPLPTTA
                     LPGLARSRM"
     gene            692..1399
                     /locus_tag="PLM79_17465"
     CDS             692..1399
                     /locus_tag="PLM79_17465"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOV88147.1"
                     /translation="MQFPDFIIHDIKAHAALCAPIEACGLIAGGQFIPCENVHEEPET
                     AFRIAPEAYLDAASRFGRVDAVVHSHINGPDHPTPSDIVGQADTAVPWGIVVVRDGVP
                     GNPFWFGDQLPIAPLAGRSFRWGVYDCYGLVRDWYRLHRGIVLPNFPREWAFWEKGQN
                     TIEENILAAGFERIPMADGPGDVVGMRILGPVTNHVALHLGGGLIGHHQVNRLSRMDD
                     SLGRWRKRVTFYARLKG"
     gene            1421..2329
                     /locus_tag="PLM79_17470"
     CDS             1421..2329
                     /locus_tag="PLM79_17470"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88148.1"
                     /translation="MMRDIYFYGRLEDRYGHHHRYNVDSPVELARCMQANYGDFYRQI
                     RGGRFQFIRGDRFRGDFLGAEHLAMKLGAAPLHVVPVPEGAGGDSKPIITAVLGVALI
                     AAAVLIPGAGQAALLAGSEGFKAAMAGGVVIPGLGCVTYGSIALLGATLALGGISQLL
                     APTPQVTDYGNREKEKTSWLFTGATNRTEQGGAIPLIYGGPIRVGSIQASFGIRVEDI
                     REPDPPLDYHIISAGASIGGVIEPSGECPVMDGANFAFQATPFEGYALSYWLVDNVEV
                     TPSGDTYTFTDVTANHSIHAHFGPVS"
     gene            2335..>2933
                     /locus_tag="PLM79_17475"
     CDS             2335..>2933
                     /locus_tag="PLM79_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88149.1"
                     /translation="MSSQPGKLLPPSLQASRLPGLSGRGGGQEGHTPTEDPNNLLANS
                     IANMVDVLGEGPIYGLADQDHKKRCIFLDDVALQTDTGVDNFEGVQIQERKGHPSQDP
                     FKNVDSIEAEVPVGVEITKAVPVVRTVTDTDIDDIRIKIMLPSLLRINKKTGDIHGTK
                     VELRFFIRRDGGTYKKVKDVKISGKTNAQYIREYRIPNIA"
     assembly_gap    2934..3033
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3034..>4601
                     /locus_tag="PLM79_17480"
     CDS             <3034..>4601
                     /locus_tag="PLM79_17480"
                     /inference="COORDINATES: protein motif:HMM:NF024939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOV88150.1"
                     /translation="DFEYTLNEPVYSGVWNGSFKRAWTCNPAWIWYDLATNDRYGLGI
                     DPSRIDKWSLYSIGQYCDAVDPTTGKFVGVSDGAGGLEPRFTMSLYLQTQEEAFHVLN
                     AVASAFVSMPYWSSGMVCLAQDAPSDPTHLANRANVINGEFQYSGLGLKARHSVALVT
                     WYDPDDACRPAVEVVEDPELIERFGWVTTDVLAVGCLSRSQAIRFGRWMLDSEKNQPE
                     TVTFRGSFYWADCMPGSLIRIQDPTYAGLRFGGLVASGSTKTSVKLDAPVTLEAGETY
                     TLSVVLTDGSIADRAVTNVPGTYTTLTVEALPSAPGANAPWVLSASNVEPREFRVLVN
                     RETAPNEFEILAVVHDSTKYARIYDNIELPAPPVSIIPTGPIEPLTNLDIQVFTHERG
                     RNFALGVLLSWEPSTDPRVLFYEIEYRESDEDDWKRFGESTTASCERRRIAEGTYDFR
                     IRAAGIGQSNWYTESSISIADPDELPPDVTGLKTRDGGNELFTGKECELVWDDMPDVS
                     DTYARWKHEHYKIQ"
BASE COUNT          891 a         1457 c         1365 g          788 t
ORIGIN      
        1 tatgctcgat gcatccggcc tgggcggcag ttactactat ttcacccagt cggcccacga
       61 gagcgccgtg gtccagtgga agcggcatcc gaacgacaac cggccagggc ctgccgacta
      121 cagccccatc gatatcgagg ccgaaggatt cgaattctcc gggcagggac agctgccgcg
      181 ccccaagttc cgcatcagca acgtgaccct ggctctcatg tccgcggtca tcgcctggaa
      241 cgatctcctg ggggccgtgg tcacccggta ccggacactc aagaaatacc tgtacgggca
      301 gccgggatat gatccggacg ctcatttccc cgaggacgtg tacgtcatcg agcgcaagac
      361 cctgcagagc aaggtcatga tcgagtggga gctctcctcg atcctggatt ttcaggggca
      421 gcagctcccg cgccgcaagg cgctgcggga ctactgtacc caccggtacc gggtatggga
      481 cccgaatgcc gaggaattca tctacaccca tgcccggtgc ccctatcgag gcaaccgtta
      541 ctatgacgcc ctcgatgtcg agcaggccgg gcaccccgag ctggaccgat gcggaaagcg
      601 gctatcgagc tgtcggctga ggtttaccgg caagcaacca ttgccgacca cggccctacc
      661 cggcctggcc cggtcgagaa tgtgaggatg catgcaattc ccggatttca tcatccatga
      721 cattaaggcc catgcggcct tgtgcgcgcc catcgaggcc tgcggtctca tcgccggggg
      781 gcaattcatc ccctgcgaga atgtccatga ggaaccagag acggcattcc gcattgcccc
      841 tgaggcctat ctcgatgcgg catctcgatt cggtcgggtc gacgccgtgg tccatagcca
      901 tattaatgga ccagaccatc caaccccgtc ggacatagtc gggcaggcgg acacggccgt
      961 gccctggggg atcgtcgtgg tgcgggacgg ggttcccggc aatccgttct ggttcgggga
     1021 tcaactcccc atagccccgt tggccgggag gagcttccgc tggggcgtgt atgactgtta
     1081 tgggctggtg cgggactggt atcgcctgca ccgcggaatc gttctgccca acttccctcg
     1141 ggagtgggct ttctgggaga aaggacaaaa cacgatcgaa gaaaacatct tggccgcggg
     1201 attcgagcga atcccgatgg ccgacggacc cggggacgtc gtcggcatgc ggatccttgg
     1261 ccccgtgacc aatcacgtgg ccttgcattt aggcggagga ctgatcggcc atcatcaggt
     1321 caaccgtctc tcccgcatgg acgattccct gggtagatgg cgcaaacggg tgacgtttta
     1381 cgccaggcta aaaggctagg ggcgaggggc tagaggcgaa atgatgcggg atatctattt
     1441 ctatggcagg ctcgaagacc ggtatgggca tcaccaccgc tataacgtgg attccccggt
     1501 ggagctcgcc cgctgcatgc aggccaacta cggagatttc taccggcaga tccggggcgg
     1561 tcggttccag ttcatccggg gagaccggtt ccggggcgat ttcctgggcg cggagcacct
     1621 ggccatgaaa ctgggggccg cacccctgca cgtggtcccg gttccagagg gagccggcgg
     1681 agactcaaag ccgatcatca ccgcggtcct gggcgtcgcc ctcattgccg cggccgtcct
     1741 catcccaggg gccgggcagg cggccctcct cgccgggtcg gaagggttca aggcggcaat
     1801 ggccggcggg gtcgtcattc ccgggctggg gtgcgtcacc tacggatcca tcgctctgct
     1861 gggcgccacc ctggcattgg gaggcatttc ccagctactg gctcccactc cccaggtgac
     1921 ggattacggc aaccgagaaa aggagaaaac ttcctggctg ttcaccgggg ctaccaaccg
     1981 caccgaacag ggcggggcca tcccactcat ttatggcggg cccatccggg tcgggtccat
     2041 ccaggcctcg ttcggaatcc gggtcgagga cattcgggaa cccgatccgc ccctcgatta
     2101 tcacatcatc agcgccgggg cgagtatcgg cggcgtcatc gagccgtccg gggagtgccc
     2161 ggtaatggac ggggcaaact tcgcgttcca ggcaactccc ttcgagggct atgccctcag
     2221 ctactggctt gtggacaatg tcgaggtgac ccccagcgga gacacctaca cgttcacgga
     2281 cgttacagca aatcattcga ttcatgccca tttcgggccg gtgagttgat agccatgagc
     2341 tcacaacccg gaaagctttt acctcccagc ctccaagcct cccggcttcc cggcctctcc
     2401 ggccgagggg gcgggcagga agggcacacg cccaccgagg acccgaacaa tctcctggcc
     2461 aattccatcg ccaacatggt ggacgtgctc ggggaagggc cgatctacgg cctggccgac
     2521 caggaccaca aaaagcggtg tatttttctc gacgacgtgg ccctccaaac cgacactgga
     2581 gtggacaact tcgagggcgt gcaaatccag gaacgaaagg gccaccccag ccaggatccg
     2641 ttcaagaacg tggattccat cgaggctgag gtccccgtcg gggtcgaaat caccaaggcg
     2701 gtgcccgtcg tccgaaccgt caccgacacg gacatcgacg acatacgcat caagatcatg
     2761 ctgccctctc tcctgcgaat caacaagaaa accggggaca tccacggcac caaggtcgag
     2821 ctgcggttct tcatccgccg ggacgggggc acctacaaga aggtaaaaga cgtcaagatc
     2881 tcgggcaaaa ccaacgccca gtacatccga gagtatcgaa tcccgaacat cgcnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngacttcg aatacaccct caacgaaccg
     3061 gtctattccg gggtctggaa cgggtccttc aagcgcgcct ggacctgcaa cccggcctgg
     3121 atctggtatg acttggcgac caacgatcgc tacggcctcg ggatcgaccc gtcccgcatc
     3181 gacaagtggt ccctctactc catcggccag tattgcgatg cggtggaccc gaccaccgga
     3241 aagtttgtgg gcgtatccga cggggccggg gggctcgagc ctcgctttac catgagcctt
     3301 tacctccaga cccaggaaga ggcgttccac gtgctcaacg ccgtggcctc ggccttcgtc
     3361 tcgatgccgt actggagctc cggcatggtc tgcctggccc aggatgcccc ctcggacccg
     3421 acccatcttg ccaaccgagc caacgtgatc aacggggaat tccaatacag cggcctggga
     3481 ctcaaggcgc gccattccgt ggccctggtg acctggtacg accccgacga cgcatgcagg
     3541 cccgccgtcg aggtcgtcga ggacccggag ctcatcgaac gcttcggatg ggtgacaacg
     3601 gacgtgctgg ccgtagggtg cctcagccgt agccaggcca tccggttcgg gcgatggatg
     3661 ctcgattccg agaaaaacca gccggaaacc gtgacgtttc gcggcagctt ctactgggcc
     3721 gactgcatgc cgggaagcct catccgcatc caggacccga cctatgcggg ccttcgattc
     3781 gggggtctgg tcgcttccgg cagcaccaaa acgtccgtca agctggacgc cccggtcacc
     3841 ctcgaggccg gagaaaccta caccctttcc gtggtcctga ccgacgggtc catcgccgat
     3901 cgggccgtca ccaatgttcc ggggacctac accaccctga cggtcgaggc gcttccgagc
     3961 gcccccgggg ccaacgctcc ctgggtgctc tcggcgagca acgtcgagcc ccgcgaattc
     4021 cgggtgctgg tcaaccggga gaccgcgccc aacgaattcg agatcctggc cgtggtccac
     4081 gattcgacca agtacgcccg gatctacgac aacatcgagc tcccggctcc tcccgtcagc
     4141 attatcccca ccggtcccat cgagccgctc acgaacctgg acatccaggt tttcacccac
     4201 gagcgcggcc gaaacttcgc cctgggcgtg ctgctctcat gggagccgtc cacggatccc
     4261 cgggtgctct tctatgagat cgaataccgc gagtcggacg aggacgattg gaagagattc
     4321 ggcgaatcca ccaccgcgag ttgcgagcgc aggcgcatcg ccgagggcac ctacgatttt
     4381 cgcatccgcg ccgccggaat cgggcagagc aactggtaca ccgaatcctc gatctccatc
     4441 gccgatccgg acgagcttcc cccggatgtg accgggctga agacccggga cggcggaaat
     4501 gagctgttca ccggaaaaga gtgcgagctg gtgtgggacg acatgcctga tgtctcggac
     4561 acgtatgctc gatggaagca cgagcactac aagatccaga t
//
LOCUS       DAOOVC010000235         4580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10440_length_4580_cov_4.780331, whole genome shotgun sequence.
ACCESSION   DAOOVC010000235 DAOOVC010000000
VERSION     DAOOVC010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10440_length_4580_cov_4.780331"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1371..1937)
                     /gene="cas6e"
                     /locus_tag="PLM79_17485"
     CDS             complement(1371..1937)
                     /gene="cas6e"
                     /locus_tag="PLM79_17485"
                     /inference="COORDINATES: protein motif:HMM:NF020377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HOV88151.1"
                     /translation="MFLSELRLAGRKLPNPYEWHRLLWNMFPEEPEKKRDFIFQVNRE
                     NGSILMLSASLPVQPESGTIRFIRSRPFVPKLAPGEILRASLRASPSKRDSKSRRRLG
                     LFDHDEQMAWLQRKMTGARVLKADIKGPEPLHFRKDRNAATLFICQFDFLIEVVDPEL
                     LIETMRKGVGPGKAFGCGLLLLQRVSVA"
     gene            complement(1924..2595)
                     /gene="cas5e"
                     /locus_tag="PLM79_17490"
     CDS             complement(1924..2595)
                     /gene="cas5e"
                     /locus_tag="PLM79_17490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HOV88152.1"
                     /translation="MTTDYLILKLQGPMQAWGGHSYENTRPTEYFPTRSGIVGLLAAC
                     LGIERARHASLRDLSESFSFAVRADQHLDEMGLASGALTDFHTVQNVPQVAGGTRTIV
                     THRQYLCDLSFTVALRFHQQGRYQMKEVLEAVSKPLYTPYLGRKSCPLARPLFECVVK
                     GADFLDALSNVEPREGLVYSEDNLGSVNILHVRDVPFFERRRFGTREVYVHYMQKEGL
                     SDVSQ"
     gene            complement(2626..3006)
                     /locus_tag="PLM79_17495"
     CDS             complement(2626..3006)
                     /locus_tag="PLM79_17495"
                     /inference="COORDINATES: protein motif:HMM:NF008912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOV88153.1"
                     /translation="MAYGSFEDLEVWKRACRLAVKVYEVLRECRDYGLKDQMTRAAVS
                     IASNTAEGAERDSKAEFIRFLHIAKGSAAELRTQVYIAHRIGFLESSHKEELIAELKT
                     LSSMLHALIKSLKHPNPPPRAPKT"
     gene            complement(3139..4188)
                     /gene="cas7e"
                     /locus_tag="PLM79_17500"
     CDS             complement(3139..4188)
                     /gene="cas7e"
                     /locus_tag="PLM79_17500"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HOV88154.1"
                     /translation="MNVHVLISHSASCLNRDDMNMQKSMTFGGVNRVRISSQCLKRAM
                     RRSPYHTARLGEGSIRTRSLELLKEKLVDHPRLERFGSADIALAIDLMGGAKASDILM
                     PWSVSEIEAACVAIEDARAKRVVDDALSSEVEKVLKKRTGLVPADVALSGRMITSGIM
                     SRIDGALSVAHAFTTHAVSSDVDWFTAMDDLTQELEDARGAAHLNTQEFGAGVFYRYI
                     SLNIGQLERNMEAGRADALNVAADYTHLLATAVPTGKQHSFAAFNVADLVLVSFADMP
                     ISAANAFETPIRENGRGYLQPSIQALEEYIETVRRGYGIDETQAVFSLRDTNLSPRLD
                     TLEELKSWIRADGEF"
     gene            complement(4282..>4580)
                     /gene="casB"
                     /locus_tag="PLM79_17505"
     CDS             complement(4282..>4580)
                     /gene="casB"
                     /locus_tag="PLM79_17505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HOV88155.1"
                     /translation="GWQRVLFLLPYIAHAENRRLGAALAVKIKEQRLFQVIRSDAPTD
                     LIHLRRICQYASPQADWQTVGEMLFYWGKGQKTRLMEDYLNALRRQSSSTDYTD"
BASE COUNT         1012 a         1228 c         1235 g         1105 t
ORIGIN      
        1 catggaggag atgcatagac tccactcctg tctttgctgg agacaaaggg aagagaagcc
       61 ctagcgattg attgaagatc ccaatcgact ccaggcccaa atactagaag cctttggcta
      121 tgaggtggtc caagggatca tgcaggaggc tgctctcaag tgattgtaac tccagtatat
      181 tttatttgct cagattttta ccccaaaaat gactggtcga aaatacccca agttgccatt
      241 ggcaacttca gggggtaatt gcactatcct tttaatagaa aaagaaagga agcctcttga
      301 atcccccaga tatgtggaac tttcgcagac gccatgatct cacaggagca gacttggctc
      361 gaatgcttgg tgttcacaaa agccaagtat cccgctggga gaccggacaa cgggaaatcc
      421 cgctatggct cgagaaattt cttgcatgtc ttgaccaaac tcttccagtg aggaagccgc
      481 tcaaagcaag caccaaccca tacgattccg gggctgctcc acaatgagtg ttcccaagaa
      541 agcgcagatt cagatcccga tatccatcgg ctgcgaattg gtgagtaggg tcttacaaaa
      601 ttccgctccg taattagctg ttttaattaa tgtttgccgg attttgacct ctctggcccg
      661 taaattcctg ttagattaca tagatcatag atccgtggaa cggtaccgat tccacagatt
      721 ccggatttga atttcaattc aatagctgca gcgcaatggc aacaccctgc atccccactt
      781 tcgtgagggg cgtatttgtt gcaggaacag ccaatttcta caacgattca tccccactca
      841 cgtgggggac acttctgacc aaggtgggcc gcacctgctc catcgattca tccccacatg
      901 cgtgggggaa tatggcgcgt tcgatggcca tcatgtcaaa catcgatgca tccccacttg
      961 cgtgagggtc caatttgata ataccaaaca ccaaaaccac catctgattc atccccactt
     1021 tcgtgggggt gctccgcgaa ttctcgaata cggataaccg gcatcgattc atccccacgt
     1081 gtgtgaggga catgccagtg tcttagcgat acggagatcc gaaagagctt catccccacg
     1141 tgtgtggggg catctgctac caataatcat aatcaacctg ccaactgata catccccacg
     1201 cgtgtggggg aaattcgcag tctgaaagcc cgcgccgttc gaaagccgat tcatccccac
     1261 gtgcgtgggg gagatgcgag aaacgtctcg ttattatcgc aaatgagcga ttcatcccca
     1321 catgagtggg ggacatattc aacagctaat aacctatcgg ataactgact tcaggcaacc
     1381 gatacccgct gcagcagtaa tagaccgcat ccgaaagcct tgccggggcc gactcctttg
     1441 cgcatggttt caattagcag ctccggatcc acgacttcaa tcaagaagtc gaattggcat
     1501 atgaacaggg tggcggcatt acgatccttg cgaaaatgca agggctcggg gcctttgata
     1561 tcagctttga ggaccctggc gcccgtcatc ttgcgttgca gccaggccat ttgttcgtca
     1621 tggtcgaata gtcccaggcg ccggcgggac ttcgagtcgc gcttggatgg gctggccctg
     1681 agggaagctc tcagtatttc gccgggcgcc agtttcggaa cgaacggcct ggaacgtatg
     1741 aaacggatgg ttccactttc cggctggacg gggagagagg ccgacaacat aaggatgctt
     1801 ccgttctcac ggtttacctg gaagataaag tcgcgctttt tctccggctc ctcgggaaac
     1861 atgttccaca acagcctgtg ccattcgtaa gggtttggca gctttcttcc ggcaagtctc
     1921 aattcactga gaaacatcgc tcagaccctc cttttgcata taatgcacgt aaacttcccg
     1981 ggtgccgaat cgccttcttt cgaaaaaggg cacatcccgc acatggagga tgtttacgct
     2041 tcccagattg tcctcactgt agacgagacc ttcacgcggc tccacatttg acagggcatc
     2101 gaggaaatcc gcccctttca cgacgcactc gaaaagcggc cgcgccaggg ggcagctctt
     2161 gcggccgagg tagggtgtat acaagggctt cgagaccgct tcgagaactt ccttcatctg
     2221 gtagcgtcct tgttggtgaa aacggagtgc cacggtaaag ctcaaatcgc acaggtattg
     2281 ccgatgggtg acaatggttc gtgttccacc ggcgacctgg gggacatttt ggacggtgtg
     2341 gaaatcggta agagctccgc ttgcaagacc catctcgtcc aggtgctgat ccgcgcgcac
     2401 ggcaaaagag aatgattcgg agaggtccct caggctcgcg tgccgagctc gctcgatgcc
     2461 aagacaggcg gcgagcagcc ctacgatccc gctgcgtgtg gggaaatact ccgtgggcct
     2521 cgtgttctcg taggaatggc cgccccaggc ctgcatcggg ccttgaagct ttaggattag
     2581 atagtctgtc gtcatggcaa aacctttggt tttaggtttt aagttttaag ttttaggtgc
     2641 tcggggtgga gggttcgggt gcttgagcga tttgatcagc gcatggagca tggaggaaag
     2701 ggttttcagt tcggcgatga gctcttcttt gtgtgatgat tctagaaatc cgatcctgtg
     2761 ggcgatgtag acttgagttc tcagctcagc agcggaaccc ttggcgatat gaagaaagcg
     2821 aatgaactca gctttggagt cccgctctgc accttctgcc gtgttcgaag ctatggagac
     2881 agccgctctc gtcatctgat ccttcaaccc ataatccctg cattcgcgca aaacttcgta
     2941 aaccttcaca gccagccgac aagccctctt ccaaacttcc agatcctcaa aagatccata
     3001 agccattttt tctcctccgg agaaaaaggt tttaagtttt aggttttaag ttttaggtgc
     3061 gaagggttgc gtgccttgct ccgttctgct taaaacctaa gacccaagtc tttgaactta
     3121 aaacttaaaa cctaaaactt aaaactctcc atccgccctg atccaggact tgagctcctc
     3181 cagggtatcg agcctggggg agaggttcgt gtcccggagg gagaagacgg cctgggtttc
     3241 gtctatgccg tatccgcgtc ttacggtttc gatgtattcc tcgagggctt ggatcgatgg
     3301 ctgcaggtag ccccggccgt tttcgcggat cggggtttcg aaggcgttgg cggccgagat
     3361 gggcatgtcg gcaaagctca ccagcacgag atcggcgacg ttgaaggccg caaagctgtg
     3421 ctgctttccc gtggggaccg cggtggccag aagatgggta tagtccgcgg ccacgttgag
     3481 ggcatcggct cttcccgcct ccatgttccg ctccagttgc ccgatattga ggctgatgta
     3541 gcggtagaag accccggctc cgaattcctg ggtattgagg tgcgcggcgc cacgagcgtc
     3601 ctcgagctcc tgggtcagat cgtccattgc cgtgaaccag tccacgtcgc tgctcaccgc
     3661 gtgggtcgtg aaggcgtgag cgacggagag ggcgccgtcg atgcggctca tgatcccgga
     3721 ggtgatcatt cttccggaaa gagcgacgtc cgcgggaacg agtccggtcc gtttcttgag
     3781 gaccttttcc acttcactcg aaagggcgtc gtcgacgact ctttttgccc tggcgtcttc
     3841 gatggccacg caggcggcct cgatttcgga tacgctccac ggcatgagga tatcgcttgc
     3901 cttggctccg cccattagat cgatcgcgag cgcgatatcc gcgctgccga agcgttcgag
     3961 cctgggatga tccacgagct tttccttcag aagctcgaga gacctggttc gaatggaccc
     4021 ttcacccagg cgggcggtgt ggtaagggct ccttctcatt gcccgcttca ggcactggct
     4081 cgatatccgc acccggttca ccccgccgaa ggtcatggac ttttgcatgt tcatgtcgtc
     4141 ccggttgaga caactggcgc tgtgcgagat gaggacgtgc acgttcacga aatttttcct
     4201 gttgcttccc attcttttct ccctcttttt ttatccacag atttcaccaa tttttcacag
     4261 attcttaagc cttgatctat gtcaatctgt gtaatctgtg gatgaagatt gtctgcgcag
     4321 agcattgagg taatcttcca tcagccgggt cttctggccc ttgccccagt agaagagcat
     4381 ttcccctacc gtctgccagt cggcttgagg ggaggcgtac tgacaaatac ggcgaaggtg
     4441 gatgaggtcc gttggagcat ccgacctgat tacctggaac agccgttgtt ccttgatctt
     4501 cacggcgagc gcggcgccaa ggcgtctgtt ttcggcatga gcgatatacg gcaggaggaa
     4561 gaggacccgc tgccagccgc
//
LOCUS       DAOOVC010000236         4565 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10476_length_4565_cov_4.157871, whole genome shotgun sequence.
ACCESSION   DAOOVC010000236 DAOOVC010000000
VERSION     DAOOVC010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10476_length_4565_cov_4.157871"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLM79_17510"
     CDS             <1..631
                     /locus_tag="PLM79_17510"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOV88156.1"
                     /translation="MEALAPGGQCAVVVPEGLLFGSTSAHVELRRKLVEDFDLLAVVS
                     LPAGVFKPYAGVKTGVLVFRKPLSGAKKDKKTKHKEKVWFYEVRADGFDPDKISGGGR
                     PETPERNDIPDLLAQWKVYKESKFSNPPGVEAGTLLQPGSDEPRCWWATIKSVAENDY
                     NLAAGRYKPQIAEKAPDEDPAQLIRETLAIEREIADGLEKLLREIGRVD"
     gene            860..1144
                     /locus_tag="PLM79_17515"
     CDS             860..1144
                     /locus_tag="PLM79_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88157.1"
                     /translation="MYEIETGFVSHIMAYHEPKPILWLMDELQRVMCRTKQEMCDSSK
                     IELNWCETKETLWPSLVVAQALGSHEPQIELWLTGEPLNRKSSMAVEAGE"
     gene            1141..2412
                     /locus_tag="PLM79_17520"
     CDS             1141..2412
                     /locus_tag="PLM79_17520"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOV88158.1"
                     /translation="MRWPVFPLDKIADVFGGSTPRRNRSEYWDGDIPWVTPTDLPMPG
                     TEIADVYDTADHITPEGLRSCAANLLPVGTVLYSSRATIGKIGIARVPLATNQGFANF
                     APKPGVESKYLAYALQYFTVEISSLAGSTTFKEVRRGALKKYKVPLPSPSEQHRIVEI
                     LDQADALRKKRAEADAKAARILPALFYKMFGDPLALVTSGQGVPLSELEVDLQNGFAC
                     GEKDVEDGVPHLRMNNIDDAGVLNLELVRTVPVDRDTERYRLKEKDVLFMGTNSEDKI
                     GKTCLFMPPDERAYLFSNHLIRLRVSDSRITPEYLAGFLHLLWSKRFFPSIAKRWVNQ
                     STVAQPSLAALRIPLPGERSLHMFTTAFQNLLSMRAQRVRSGETLDQTFAVLLHRAFT
                     GDLTAKWREAHMKELLQEMEQQAKELEIKEP"
     gene            2409..4103
                     /locus_tag="PLM79_17525"
     CDS             2409..4103
                     /locus_tag="PLM79_17525"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88159.1"
                     /translation="MRILKVHIENYRSLKNFTLEPGPFCVLIGENNAGKSNILRALNL
                     VLGDTWPSERSFSEEDFYNQDTSQDILIQVFFDETIEDWRNNHKAEIHGIQLRCKAYK
                     RAVKGKPAGSLKVDFTCINENGEEVTHPEEPLQKGQQYKGRWVPVRVSGELRERLPFI
                     YVEVLRDYNRQTPGSRWSVLRRLFNEVNTEFLNDKKEVKVPQSDGPTLMMTRKEAFTR
                     AVRDAYKYLRTESFEEIEKRLAQNAMEHMGLAEGEGKIELHFESHDPTNAYKSLQLYV
                     QQMGIESPAGEVGAGLQSAIVVAIFRTYEEMKKEGAIFAIEEPEVFLHPQKARYFQTV
                     LERLSEAGNQVFLTTHSPIFVQVHKPESVAVIRRTADKGTEACQAQACDFTDSERQTL
                     RLLTEFDAERKELFFAKAVLLVEGATEKVALPLAFKAMGYDINRLGISVVEVGGKTKF
                     PLFVRVLKALHIPYAVLADQDIKIIDVTMQESVKKKISVNNAKHKRWNEDIERVCEAD
                     SLFWMKPNFELELGLPKEESEKIDQAMEMFSNVTKSNIPQSLIDPIESVVNKTRST"
     gene            4116..>4565
                     /gene="rhuM"
                     /locus_tag="PLM79_17530"
     CDS             4116..>4565
                     /gene="rhuM"
                     /locus_tag="PLM79_17530"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOV88160.1"
                     /translation="MTKLPIKQPRSELLIYQTEDGRTRIQVRLEHETVWLTQADMAQL
                     FQNTPQNITLHLKNIFQEGELNERATCKDFLQIQVEGGRQVHRSRKFYNLDAIISVGY
                     RIKSHVATRFRQWATQRLREYIVKGFTLDDERLKQVGGGNYFDELLAR"
BASE COUNT         1203 a         1150 c         1201 g         1011 t
ORIGIN      
        1 gatggaggcc ctggctccgg gcggccagtg cgctgtggtg gtgcccgaag ggctcctttt
       61 cggctcgacc tcggcgcacg tggagctgcg ccgcaagctg gttgaagatt tcgacctctt
      121 ggccgtggtt tccctgcctg ctggagtatt caagccatac gcaggcgtga agacgggagt
      181 cctggtattc cggaagccac tttcaggagc aaagaaggat aaaaagacca agcataagga
      241 aaaggtctgg ttctacgagg tgcgtgcgga cggcttcgat ccggacaaga tttccggcgg
      301 cggccggcct gaaacccccg aacgcaacga cattcccgat ctgcttgcac agtggaaggt
      361 ctataaggaa tcgaaattct cgaacccgcc gggtgttgag gccggaaccc ttttgcaacc
      421 cggaagcgac gagccgcgct gctggtgggc aaccatcaaa tctgttgccg aaaacgatta
      481 caaccttgcc gccggccgct acaagccgca gatcgccgaa aaggccccgg atgaggaccc
      541 ggcccagctt attcgggaaa cgctcgccat cgagcgggag atagctgatg gtttggagaa
      601 attgcttaga gaaatcggga gggtggattg atgattgatg atttaagatt gatgattgaa
      661 aaccaggcct tacaaccttc gatcataaat caaaaatctg agatggactc gctgctaaag
      721 gagctagagc aataatgaag aaaccataca ctcctcaaaa attaccgact gcatccgctt
      781 atggagggaa aaggaaggta gggtacaaaa tggatattct tgcctttgct gagaatcatg
      841 agcatggagg acctggatca tgtatgagat agaaacaggt tttgtgtctc atataatggc
      901 atatcatgag ccaaaaccga ttttgtggct catggatgag ctacaaagag taatgtgtcg
      961 cacaaaacaa gaaatgtgcg attcaagtaa aattgagctg aattggtgcg aaacaaaaga
     1021 aacattgtgg ccttccctcg ttgtggctca agcacttgga tcccatgagc cgcaaatcga
     1081 attgtggctt acaggtgagc cgctaaatcg aaaatcttcg atggccgtgg aggcaggaga
     1141 atgaggtggc ctgtgtttcc attagataaa attgcggatg tctttggtgg tagcacccct
     1201 aggcgaaacc gttctgaata ttgggatggg gatatccctt gggtaacgcc gacagatctc
     1261 cctatgccag gcacggaaat cgccgacgtt tatgacacgg cagaccacat aacgccagag
     1321 ggtctacgtt cgtgcgcagc caaccttctt ccggtcggaa ccgtcctcta ttcctcaaga
     1381 gctaccatcg gaaagatagg tattgctcgc gtcccgttgg ccacgaatca agggttcgcc
     1441 aatttcgccc caaaacctgg agtggagtca aagtacttgg cttatgccct ccagtatttc
     1501 acagtcgaga tatcttcact ggctggctct acaactttca aagaagtccg tcgtggtgcc
     1561 cttaagaaat acaaagtgcc cttgccgtcc cccagcgagc agcaccgcat tgtcgagata
     1621 ctcgaccagg cggatgcctt gcgcaaaaag cgcgccgagg ccgacgccaa agccgcccgc
     1681 atcctccccg ccctcttcta caaaatgttc ggcgatccgc tggcgttggt gacttccggc
     1741 cagggtgtcc ctctttctga gcttgaagta gacctgcaga acggattcgc ctgcggcgaa
     1801 aaggacgttg aagatggtgt tccgcacctc cgcatgaaca acatcgacga tgcaggcgtg
     1861 ctcaatctgg agcttgtgcg gaccgttcct gttgaccgtg ataccgaacg gtatcggcta
     1921 aaggagaaag acgtgctttt catgggcact aacagcgagg acaagatcgg gaagacctgt
     1981 cttttcatgc ctcccgatga acgagcctac cttttcagca atcatctgat ccgccttcgc
     2041 gtatcagact cacgcattac cccagagtac cttgccggtt tcctccacct gttatggtcg
     2101 aaaaggttct tcccttctat tgccaagaga tgggtcaacc aatctaccgt ggctcagcct
     2161 tcacttgcag cccttcgcat tcctttgccg ggcgaaaggt cgctccatat gttcacgact
     2221 gcattccaaa atctcttgtc tatgagggca cagcgggtgc gctcaggaga aaccctcgac
     2281 cagacctttg ccgtcttgct tcatcgcgcc tttaccggcg acctgacggc caagtggcgc
     2341 gaggcgcata tgaaggagct cttgcaggag atggagcagc aggcgaaaga gctggagatt
     2401 aaagagccat gagaattttg aaggttcata tcgagaatta ccgctccctt aagaacttca
     2461 ctcttgagcc agggccgttc tgcgttctca tcggtgaaaa caacgccgga aaatcaaaca
     2521 tattgcgggc gcttaatttg gtcctcggtg acacctggcc ctcggaaagg agcttttcgg
     2581 aagaggattt ctacaatcag gataccagcc aagacatact gatccaagta ttcttcgatg
     2641 aaacgattga ggattggcgc aataaccata aagcggaaat tcatggaatt cagcttcgct
     2701 gtaaggctta caagcgggcg gtaaagggca agccagcagg gtctctgaaa gtggatttta
     2761 cctgcatcaa cgaaaatgga gaagaagtta cacacccgga agagccgcta cagaaggggc
     2821 agcaatataa agggcgttgg gtgcccgtaa gggtgtcagg cgaattaagg gaaaggctcc
     2881 cttttattta tgtcgaagtg cttcgtgact acaaccgcca gacgccggga agccgctggt
     2941 ccgttcttcg gcggcttttc aacgaggtca atacggaatt tcttaatgac aagaaggaag
     3001 tcaaagttcc tcagtctgac ggtccaaccc tgatgatgac gcgaaaggaa gcctttacac
     3061 gagccgtacg tgatgcctac aaatacttgc gaaccgaatc tttcgaagaa atagagaagc
     3121 ggcttgctca aaatgccatg gaacacatgg gattggctga aggcgagggc aagattgaac
     3181 ttcactttga aagccatgac ccgacaaatg cctacaagtc gctccagctt tatgtccaac
     3241 agatgggcat cgaaagccct gccggagagg tgggggccgg tttgcagagc gccatcgtgg
     3301 tcgctatttt cagaacctat gaggaaatga agaaagaggg tgctatcttc gccatagaag
     3361 agcctgaagt ttttttgcat cctcaaaaag cccgctattt ccagacggtt ctcgaaagac
     3421 tttccgaagc gggcaatcag gtttttctga ctacccactc cccaatcttc gttcaagtcc
     3481 acaagcccga gtccgttgcc gtcattcgcc ggaccgctga caaaggtaca gaagcatgcc
     3541 aggctcaggc atgtgacttt acagattctg aacggcaaac ccttcggctg ctgacagaat
     3601 tcgacgcaga acggaaggaa cttttttttg ccaaggccgt gcttctggtc gagggagcaa
     3661 ctgagaaggt cgctctgccc ctcgcattca aagcaatggg ctacgacatc aaccgcctgg
     3721 ggatatccgt tgttgaggtt ggcggtaaga ccaaatttcc tcttttcgtc cgcgtgctta
     3781 aagccttgca tatcccgtat gccgtcctgg cagatcaaga catcaaaata attgatgtga
     3841 ccatgcagga aagcgtgaaa aaaaagataa gtgtgaacaa tgcaaagcat aaacgttgga
     3901 acgaggatat cgaaagggta tgtgaagctg attctctatt ttggatgaag ccaaactttg
     3961 aattggagct cggactgccg aaagaggaaa gcgaaaaaat cgaccaggcg atggaaatgt
     4021 tctccaacgt gacgaaaagc aatattcctc aatccttgat tgatccgatt gaatctgtgg
     4081 ttaacaagac tcgatcaact taacaaaggc acgtaatgac caaacttccg atcaaacagc
     4141 cccgctccga actcctgatc taccagaccg aagacggccg cacccgtatt caggtccgcc
     4201 tggagcacga aacagtctgg ttgacgcagg ctgatatggc tcagcttttt cagaacacgc
     4261 ctcaaaacat cactctccat ctcaaaaata tctttcagga aggggagttg aacgagaggg
     4321 caacctgtaa ggatttctta caaattcaag tcgagggggg aaggcaggtt caccgaagcc
     4381 gcaaattcta caatctggat gccattattt ccgtgggcta ccgcatcaag agccatgtgg
     4441 ccacccgctt ccggcagtgg gccacccagc gccttcggga atacatcgtc aagggattca
     4501 ctctcgatga cgagcggctc aagcaggtcg gcggcggtaa ctatttcgat gaactgctcg
     4561 cccgc
//
LOCUS       DAOOVC010000237         4547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10531_length_4547_cov_3.319679, whole genome shotgun sequence.
ACCESSION   DAOOVC010000237 DAOOVC010000000
VERSION     DAOOVC010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10531_length_4547_cov_3.319679"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="PLM79_17535"
     CDS             complement(<1..1718)
                     /locus_tag="PLM79_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV88161.1"
                     /translation="MIPSVLAHQVQQGVKDFLRTTFPLSSPHFHGLLEGLLARDGGLF
                     RGPYLSINLPFRQGKGGADFFPHVPLKFPPYFHQEQAFLRLSGDRPRSTLVATGTGSG
                     KTECFLYPILDACYRHRGEPGIKAILIYPMNALATDQAGRLARTIHGNEKLRGHVTAG
                     LFVGQSEKDPSMGMGPESIVTNKETLRLKPPDILLTNYKMLDYLLIRVKDFPLWKQNG
                     PESLRFLVVDELHTFDGAQGTDLACLVRRLKARLKTPPGFLCCVGTSATLGSEGEKQR
                     LVQYAREVFGEPFDGDSVITESRVSAGEYLADCFVTRRDVVQPEKADALDPEAYDGHE
                     AFIRAQHALWFHDEIPEGGFEEPGWRTALGEKIKGHYFFQNLLKAMEGKPQGLGELLD
                     RLERVTPELQGAPAEYRENLLNSLLSLVSEARVWGGGSEGEGGKGIPEADRKTAPFLH
                     VRIQLWLRELRRMVGEVSQTPALTFADDLNDEQLKRHLPVVNCRECGSTAWAGLKRQK
                     DSFISGNLEDFYPAFFGDDPKVTFLFPEEAGARNRPMESGISLLCPQCLNLTERLDGE
                     NCPSCNG"
     gene            complement(1752..1991)
                     /locus_tag="PLM79_17540"
     CDS             complement(1752..1991)
                     /locus_tag="PLM79_17540"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOV88162.1"
                     /translation="MRVVKVSSRGQIVVPKEIREKLGIKPGTRVLFQVVEQHAEIVPL
                     PQKPVKALRGMLKSESSLAAELLEERRQAEKGVRY"
     gene            complement(2110..3555)
                     /locus_tag="PLM79_17545"
     CDS             complement(2110..3555)
                     /locus_tag="PLM79_17545"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88163.1"
                     /translation="MKDLFDRSEILGVLHGFNPWWSGRQNPVPDFRRLALEACRIYLE
                     DASLRRAVLLSGPRRVGKTTILLQIANHLISKGHDPKSVFYVSLDHPLIKLLNVREII
                     NLYHDSIHARGKEAFLLLDEVQYSREWETEIKLLVDHQPHYRILATGSAGVVHRERLA
                     ESGTGRWITVPIPTLSFFEFVQIRGEAGPEIPASLRPTDLFSMTQGDRTELTLRLQGL
                     LPLFQHYLLVGGFPETAVQRNTALCQRLLREDVVERVLKRDMTALFGVRNVNEVERLF
                     IYLCLHSGGIVAHRTCADTLGTSPTTVANHLSLLEQANLIYRLPPAEMRGKKVLRSRN
                     KYYLVDAALRNAVLLRGEEIFTNPEEMGVIVETTVLRHLYAYYYRDVPTISYWRDPAT
                     QKEVDIIVQSPRYTFPFEVKYQENPRLREASGLVAYCKLEKVTRAYWVTKQDRDFDVL
                     RFEGLETEFLKVPAHVLCYLLGQAERLLWSS"
     gene            complement(3913..4365)
                     /locus_tag="PLM79_17550"
     CDS             complement(3913..4365)
                     /locus_tag="PLM79_17550"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV88164.1"
                     /translation="MAHQRNLRKGRFSRPGDFTFITTGTKGKDPVFSDTTAATIILDC
                     LKWLNDQSRIHLIAAVVMPDHIHFVARLLNTTLPSLMHSLKSFTANRINERLGRVGPL
                     WEPQYYDHAVRSEDELRECVRYCLENPARKGLIKDFKAYPHWYCVYEV"
BASE COUNT          908 a         1333 c         1328 g          978 t
ORIGIN      
        1 ccgttgcagg aggggcagtt ttccccgtcc aggcgttccg tgaggttcag acactgggga
       61 caaaggaggg agatccccga ctccatgggc cggtttcggg cgccggcctc ctcggggaaa
      121 agaaaggtta ccttgggatc gtccccaaag aaagcgggat agaagtcctc caggttcccg
      181 gaaatgaagg aatccttctg acgtttcagc ccggcccagg cggtgctccc acactccctg
      241 cagttcacca ccggcagatg gcgcttcaat tgttcgtcgt tcaggtcgtc cgcaaaggtg
      301 agggcgggcg tttggctcac ctcgccgacc atccggcgaa gctcccgcag ccacagctga
      361 atccgcacgt gcaggaaagg ggccgtcttc ctgtccgctt ccggtatgcc ttttccgcct
      421 tcaccttctg agccgccacc ccaaaccctt gcctcggaaa cgaggctcag caggctgttg
      481 aggagattct cccggtactc cgccggcgcg ccctgcagtt ccggtgtgac ccgctccagc
      541 cggtcgagga gttcgccaag accttggggt ttcccctcca tcgccttgag aagattttga
      601 aagaagtagt ggcccttgat cttctcaccc agggctgttc tccatccagg ttcctcgaac
      661 ccgccttcgg gtatctcatc atggaaccag agcgcatgtt gggctcgaat gaaggcttca
      721 tggccgtcgt aggcttcggg atcgagtgcg tcggcctttt caggctgcac cacgtcacgc
      781 ctcgtcacga agcagtccgc aaggtactcc ccggcactca cccgggactc ggtgatcacg
      841 gaatccccgt cgaaaggctc cccgaagact tcccgggcgt actgcacgag gcgctgcttt
      901 tcgccctcgc ttcccagggt tgccgacgtc cccacgcagc agaggaaacc gggaggggtc
      961 ttgagcctcg ccttgagccg acgcaccagg caggcgaggt ccgtcccctg ggccccgtcg
     1021 aaggtgtgga gttcgtccac cacgaggaaa cggaggctct ccgggccgtt ttgtttccac
     1081 aaggggaagt ccttgacgcg gatcagcagg taatcgagca tcttgtagtt ggtgaggagg
     1141 atgtccgggg gcttgaggcg cagggtttcc ttgttcgtca cgatgctttc cggccccatg
     1201 cccatggagg ggtccttttc gctctgcccc acgaacagtc ccgccgtgac gtgtccccgg
     1261 agtttctcgt tcccgtggat ggtcctggcc aggcgcccgg cctggtccgt cgccagggcg
     1321 ttcatggggt agatgagaat ggccttgatc cccggttccc cccggtggcg gtaacaggca
     1381 tccagaatgg ggtagagaaa acactcggtc tttccggaac cggttcccgt ggccaccagg
     1441 gttgaacggg ggcggtcccc cgaaaggcgc aggaaggcct gttcctggtg gaagtatgga
     1501 ggaaacttca gcgggacgtg gggaaagaaa tccgctcccc ctttcccctg ccggaatgga
     1561 agattgatgg aaaggtaggg tccccggaaa agaccaccgt ccctcgcgag gagaccttcg
     1621 agaagcccat ggaaatgagg gctggaaagc gggaaggtgg tcctaagaaa atccttgacg
     1681 ccctgctgaa cctggtgggc gagaaccgat gggatcatgg cagcgctttc agctcctcga
     1741 ttacaatcac ctcaatatcg gacacctttt tctgcctgac gtctttcctc cagaagctcc
     1801 gcagcgaggg acgattccga tttcagcatg ccccttaatg ccttcaccgg tttctggggg
     1861 agcgggacga tttcggcgtg ctgctccacg acttgaaaca gcacacgtgt ccccggcttg
     1921 attcccagct tctctcgaat ttccttggga acaacgattt gccccctgga cgacaccttc
     1981 accacccgca tgtgtacgtg cctcccctct gccaatgcgg ctttctcagc aagaatatta
     2041 gtccggatcg atgaagaccg cacctgcatt tatctcgccg catgaagaaa cggcgccgcg
     2101 ggatgcccct catgatgacc agaggagccg ctcggcctgc cccaggaggt agcagaggac
     2161 atgggccggt actttcagaa actccgtctc cagcccctca aacctgagga catcgaaatc
     2221 cctgtcctgc ttcgtgaccc agtaagccct cgtgactttt tccagtttgc agtaggcaac
     2281 gaggccgctg gcttcccgca accttgggtt ctcctggtat ttcacttcaa aggggaacgt
     2341 gtacctgggg ctttggacga tgatgtcgac ttccttttgg gtcgccggat cacgccagta
     2401 ggaaatggtc ggaacgtccc ggtaatagta tgcgtagaga tgcctcagga cggtggtctc
     2461 cacgatgacg cccatttcct ccgggttggt gaaaatctct tccccacgca acaggacggc
     2521 attacgcaga gctgcgtcca ccaggtaata cttgttccgg ctcctcagga ccttcttccc
     2581 gcgcatttca gccggcggca ggcggtagat gaggtttgcc tgctcgagca ggctcaaatg
     2641 attcgcgacc gtggtgggcg aagtgccgag tgtatcggca cacgttctgt gggcgacaat
     2701 gcctccgcta tggaggcaga ggtaaatgaa cagtctctcc acttcattga cgtttcgaac
     2761 cccgaagagg gccgtcatgt cacgcttgag cacgcgctcc accacatcct ccctcaggag
     2821 gcgctgacaa agcgctgtat tcctctggac ggccgtctcg gggaatcccc cgaccagcaa
     2881 gtaatgctga aaaagaggga gcagaccctg gagccgcaaa gtcagttcgg tacggtcccc
     2941 ttgagtcatg gaaaacagat cggtcggacg aagggatgcg ggtatctcag gacccgcctc
     3001 tccacgaatc tggacgaatt caaagaagga gagggtcggg atggggacgg tgatccacct
     3061 tcccgtgccg ctctcggcga ggcgttccct gtggaccacg ccggcggaac cggtggcaag
     3121 gatcctgtag tgtggctggt gatcaacaag gagcttgatc tcggtttccc actcccttga
     3181 atactgcact tcgtccagaa gaaggaaggc ttccttgccc cgggcatgga tggaatcatg
     3241 atagaggttg atgatctccc gcacattgag gagcttgatg agcgggtgat ccaggctcac
     3301 gtagaacacc gatttgggat catggccttt ggagatcaaa tggttggcaa tctgcaggag
     3361 gatggtcgtc ttcccgaccc gcctcgggcc tgagaggaga accgcacggc gcagcgacgc
     3421 gtcttccagg tagatgcggc aggcttccaa tgccagccgt cgaaagtcgg ggacgggatt
     3481 ctgcctccca gaccaccacg ggttgaaccc atgcaggact cccagtattt cacttctatc
     3541 gaatagatct ttcaaaggaa gcctccaaat cgctatttag cacatgataa gcatactata
     3601 ctttatctat tttggacaac atagcgattt tgcgacgctt gaagagtcgt atttcctact
     3661 tgggatgctc atggtgtcga gtctgctgca gcttctcggc cttgcccggg acgcacgcga
     3721 gatttgagag cctcacgcga tcctgaaggc atcgcatctt tcagcggacg accgtgagct
     3781 cgctcccatt ggcgccgcct ttgtttaccc cttccatctc ggcgataaac caacgagtta
     3841 ggaacggctt ccagccgcga accagaccca tcatatcaaa gacgtaggag cggcttccag
     3901 ccgcgaacca aactacacct cataaacaca ataccaatgg ggatacgcct tgaaatcctt
     3961 gatcaaccct tttctcgcgg ggttctccag gcaatagcgc acgcattccc ggagctcatc
     4021 ctcacttcgg accgcgtggt cgtaatattg gggctcccac agggggccga ctcttcccag
     4081 ccgctcattg atccggttgg cggtgaaact tttcaacgaa tgcatcaacg atggaagcgt
     4141 tgtgttgaga agtcgcgcca cgaaatggat gtgatccggc atgaccacgg cggcgatcag
     4201 gtggatccgc gattgatcgt tcagccattt cagacagtcc aggatgatcg tggcggcggt
     4261 ggtatcggaa aacaccggat ctttcccctt tgtgccggtg gtgatgaagg tgaagtcgcc
     4321 gggtctcgag aagcgccctt tgcggagatt cctctgatgc gccataaggt cctcgttgcg
     4381 gttggggatc tcgggtggat gttgaacatc gcggccggaa gtccaagttc gcggtttgat
     4441 gttgatcttc gcaagccggt gttgatcttc gcggcctgac cttgatcttc gcaagccgat
     4501 gttgatcttc gcggctggaa gccgctccta cggggggtgg ggggtgg
//
LOCUS       DAOOVC010000238         4488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10676_length_4488_cov_4.858561, whole genome shotgun sequence.
ACCESSION   DAOOVC010000238 DAOOVC010000000
VERSION     DAOOVC010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10676_length_4488_cov_4.858561"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1720
                     /gene="pgm"
                     /locus_tag="PLM79_17555"
     CDS             68..1720
                     /gene="pgm"
                     /locus_tag="PLM79_17555"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HOV88165.1"
                     /translation="METHELAGRPVPPSMVVNIPKLVSAYYTLTPNVEEAGQRIAFGT
                     SGHRGSSLRRSFNEPHILAISQAIVEHRRMRAVTGPLFLGMDTHALSEPALASALEVF
                     AGNGVTLLVQKGFRYTPTPVVSHAILTYNRGRRDGQADGVVITPSHNSPEDGGFKYNP
                     PHGGPAETEATRWIERRSNEILAGNLRDVRRVPFERAVKAEGTREYDFITPYVEDLSA
                     CVHMEAIAGAGLKIGVDPLGGAAVDFWEPIARRYGLSLEIVHPYVDPTFSFMTLDKDG
                     KVRMDCSSPYAMQRLIGLKDRFDIAFGNDPDVDRHGIVTRGAGLLNPNHYLSAAVAYL
                     FQNRPQWGLQAAVGKTLVTTSMLDRVSAALGRPLCEVPVGFKWFVDGLLAGSFGFGGE
                     ESAGAAFLRQDGTVWTTDKDGIIMDLLACEMTARTGKDPAVLYGELEARFGKAYYTRI
                     DTPASPGKKAVLKKLTPEMVSARELAGEPITEKLTRAPGNNAPIEGLKVVAPNGWFAA
                     RPSGTEDIYKIYAESFKDQDHLEWILNEARGIVDQAFEAAGA"
     gene            complement(2300..2860)
                     /locus_tag="PLM79_17560"
     CDS             complement(2300..2860)
                     /locus_tag="PLM79_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88166.1"
                     /translation="MEWKVAAIVLIIQIIVSVVSYRWWKRYESRFPGVREELLDSLKS
                     HLEKGTLEDFVAAENVVPSPLLGKQQARRLYLRTGLAMIVALAVSFLAAKVFASYLVL
                     RAIVCTLVIMPFVHLLTKIIAFEADEIRIVEFQEVLRERIADQMAGGDVEGYVKELLE
                     AGISGLTLPAGSRGIGRLPSQGPAHS"
     gene            complement(3100..4074)
                     /locus_tag="PLM79_17565"
     CDS             complement(3100..4074)
                     /locus_tag="PLM79_17565"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOV88167.1"
                     /translation="MINDTVTSIEGILVGHAEQTGVASGCTVLLPRGGATAGVDIRGG
                     APGTYGTDTLHPLNLVDRIHALFFTGGSAFGISVAGGVRSFLHERGIGFDSGHGFVPI
                     VAGAVIFDLALNPQGRYPDAETGYRACIRASGEPVAEGNVGAGTGASVGKLYGMERAM
                     KGGLGCACIHAPTGVKVGALMVVNAFGDILDSRTHEIIAGCRDGEHSLGLVDAGKEIR
                     NLVCMRGFPEGQATIVGTVVTNVRFNKTQLTKIAQMAHDGLARTVNPAHTLYDGDTIF
                     ALSCGEIEGVEVSVVGALAAEATAQAIERGVRKAGPLGTLPSFADLNR"
     gene            complement(4064..>4488)
                     /locus_tag="PLM79_17570"
     CDS             complement(4064..>4488)
                     /locus_tag="PLM79_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88168.1"
                     /translation="AESGALEAARVLQSLPMTREETRCICQAIRNHEAFVAPVVSDRP
                     WFQLVSDCLYDADKFRWGPDTFTHTLWHMMDHQKLSAHELAAKFPWGMTGIRRVMETF
                     RTATGRQYGPEIIEIGMEIGKEVYRYLLQFIRDNSHDQ"
BASE COUNT          875 a         1430 c         1334 g          849 t
ORIGIN      
        1 ctacaaggaa tggagatgag cctcccggga ggtggaaccc aacccggaca ggaatggaga
       61 acgccctatg gaaacacacg agctggcagg aagacctgtt cctccttcga tggtcgtgaa
      121 catcccgaag ctggtttcgg cctactacac cctgacgccg aacgtggagg aggccggaca
      181 aagaatcgct ttcgggactt cggggcatcg gggatcttcg ctgaggagga gttttaacga
      241 accgcatatc cttgccatca gccaggccat tgtggagcat cgtcgaatgc gggcagtgac
      301 gggccctctc ttcctgggga tggacaccca cgccctctcg gagcccgccc tggcgagcgc
      361 cctcgaggtc tttgccggca acggcgtgac cctgctggtt cagaagggtt ttcgttacac
      421 gcccaccccc gtggtgtccc acgcaatcct tacttacaac cggggccgaa gagacgggca
      481 ggccgacggg gtggtaatca ccccgtccca caactctccc gaagacgggg gattcaagta
      541 caatcctcct cacggcggtc ctgcggaaac ggaggccacc cggtggatcg aaaggcggtc
      601 caacgagatc ctggcgggga acctgcggga cgtgcgcagg gttcccttcg agcgggccgt
      661 caaggcggaa ggcactcgcg aatacgactt cattaccccc tatgtggagg atctttccgc
      721 ctgcgtgcac atggaggcca tcgcgggggc gggattgaag attggagtcg atcccctggg
      781 aggagccgcc gtcgatttct gggaacccat cgcccgtcgc tacgggcttt ccctggagat
      841 cgttcatccg tacgtggatc ccaccttctc cttcatgacc ctggacaagg acgggaaggt
      901 ccgcatggac tgctcgtccc cctatgccat gcaacggttg atcggtctta aagatcgctt
      961 cgacatcgct ttcggcaacg acccggatgt ggaccggcac gggatcgtca ccaggggggc
     1021 gggcctgttg aacccgaatc attacctgtc cgccgccgta gcctacctgt ttcagaaccg
     1081 cccccaatgg ggcctgcagg cggcggtggg caagaccctt gttacgacct ccatgctgga
     1141 tcgggtttcc gcagcgttgg gccgcccgct ttgcgaggtg ccggtagggt tcaagtggtt
     1201 cgtggacggg ctccttgccg gttcctttgg ttttggaggc gaggaaagcg cgggggcggc
     1261 cttcttgagg caggacggca ccgtctggac cacggacaag gacggcatca tcatggatct
     1321 tctggcctgc gagatgaccg cccgcaccgg aaaggacccc gccgtcctgt acggggaact
     1381 ggaagcccgt tttggaaagg cctactatac gaggatcgat acccccgctt cccccggtaa
     1441 aaaagcggtt ctaaagaaac tcacccccga gatggtttcc gccagggaat tggcgggcga
     1501 acccatcacg gaaaaactga ctcgcgcgcc cggaaacaat gcccccatcg aagggttgaa
     1561 agtggtcgcg cccaacgggt ggtttgcagc acgcccctcg ggaaccgaag acatttacaa
     1621 aatctacgcc gagagcttca aggatcaaga ccatttggag tggatcctca acgaggcccg
     1681 gggaattgtg gatcaggcct ttgaagccgc gggcgcatag gaactcgctc ccggaaaagg
     1741 ctcataaaca tggagtgaag atgtactgta cgaagtgtgg agaagctctc gattcccaag
     1801 atcggttctg cagacattgc ggcgaatccc gtcctccaga ctccgcaaaa cgattcgaag
     1861 ccgcatttcc tccttccgtc gggccgtccg aggccgggta ctggcacacc tatgtgcgcc
     1921 ctttttttat gacggcgggc ctgttcattg cgggcctgtg tggtttcttc ctggtcttga
     1981 aactgctgtg ggcgttttgg gggggcgaat gagtgcgcgc ccaccgggaa acggcctccg
     2041 ggaacgatcg atcctgcttt cccggcgccc aaaccggcag gccgcttctt tggaaaacgc
     2101 ccggtttctt cctctctcgc tcccgcatcc ggtacgaccc ggaagggagc caaaggccat
     2161 agaatcattc ctatccgggt attccggatg gttatccagg cttagggaat tgcctgcgta
     2221 gagtcgcggg ttcgtcgccg caagccccgc catggttgcg gcaacgaccg gcgaaaggaa
     2281 aaggcgggcg gcgcgggcct tatgagtggg cggggccctg ggacggtagg cgtccaatcc
     2341 cacgggaccc ggccggtagg gtcaatccgg aaatgcccgc ttccagaagc tctttgacgt
     2401 agccctcaac atcccccccg gccatttggt ctgcgatgcg ctcccgcaaa acctcctgga
     2461 actcgacgat tcggatctcg tccgcctcga aggcaatgat cttggtgagc agatgaacga
     2521 agggcatgat gaccagggtg cagacaatgg ccctcaggac caggtaggag gcaaagacct
     2581 tcgcggccag aaagctcacg gccagggcga caatcatggc cagcccggtt cgaagataga
     2641 gccttcgggc ttgctgcttc ccgagaaggg ggctcgggac cacattctcg gcggcgacga
     2701 agtcctctaa ggtccctttc tcgaggtgag acttcaagga atccagaagc tcttccctga
     2761 ccccgggaaa ccggctctcg tatcgtttcc accaacggta gctgaccacg gacacgatga
     2821 tttgaatgat cagtacgatt gcagcaacct tccattccat ctcttagctc cgcatgttcc
     2881 ggctcgtgtg ggccctcccg gccgcgaccc gtcaaccgcc gacggaggcc gacggtcgcg
     2941 aactgtcgga attggcaaag cccctatata ctcgcttttc cagacgcggg ccagagaaaa
     3001 ctccgggggc ggcacgcgac ccgtaacgtc gctttccagg ccgcttttcc ggattcggtc
     3061 caagccctca aagcggtggc agcacaggga cggcacgatc tatctattga gatcggcaaa
     3121 agagggcagg gtccccaggg gaccggcctt ccgaacgccc cgctcaatgg cctgggccgt
     3181 cgcctccgcc gccagggccc ccaccacgct gacctccact ccctcgatct cgccgcagct
     3241 cagggcaaag atcgtgtctc cgtcatagag ggtgtgggcc ggattgacgg tgcgggcgag
     3301 cccgtcgtgg gccatttggg caatcttcgt caactgagtc ttgttgaacc gcacattggt
     3361 cactacggtc cccacgatgg tggcctggcc ctcgggaaac cccctcatgc agacgagatt
     3421 ccgaatctcc ttgccggcgt ccacgagccc gaggctgtgt tcgccgtccc ggcacccggc
     3481 gatgatttca tgggtacggg agtccaggat gtccccgaac gcattcacca ccatcagggc
     3541 tccgaccttg actcccgtgg gtgcgtgaat gcacgcgcac cccagcccgc ccttcatggc
     3601 ccgttccatc ccgtacagct tccccacaga agccccggtg ccggctccga cattcccctc
     3661 ggccacgggc tccccgctcg cgcggatgca ggctcgatag cccgtctccg catccgggta
     3721 tcgcccctga ggattcaggg ccaggtcgaa aatgacggcg cccgccacaa tggggacaaa
     3781 gccgtggccg ctgtcgaatc cgatgcctct ctcgtgcagg aatgaccgaa cgcccccggc
     3841 cacactgatg ccgaaagcgc ttccgcccgt aaaaaacaat gcgtggatcc tgtccaccag
     3901 gttcagggga tgcagtgtgt ccgttccgta cgttccgggg gcccccccac gaatgtccac
     3961 tccggccgtg gctcctcccc ggggcaacaa cacggtacaa ccgctggcaa ccccggtttg
     4021 ctcggcatgg cccaccaaaa tcccctcgat ggatgtgacg gtgtcattga tcatggctgt
     4081 tatcccgaat gaattggagt aaataacggt agacttcctt gccgatttcc atccctatct
     4141 cgatgatctc gggcccgtac tggcggcccg tcgccgtccg aaaggtctcc atcactctgc
     4201 ggatccccgt catcccccac ggaaattttg ccgccagctc gtgcgcactc agcttctgat
     4261 ggtccatcat gtgccacaag gtgtgggtga aagtgtccgg tccccagcga aacttatccg
     4321 catcatacag gcagtcgctg acgagctgaa accacgggcg gtccgagacg accggcgcca
     4381 caaaggcctc gtggttccga atcgcctggc agatgcaccg ggtctcctcc cgggtcatgg
     4441 gaaggctctg cagcaccctg gccgcctcca gggccccgga ctccgcat
//
LOCUS       DAOOVC010000239         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10813_length_4438_cov_5.844627, whole genome shotgun sequence.
ACCESSION   DAOOVC010000239 DAOOVC010000000
VERSION     DAOOVC010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10813_length_4438_cov_5.844627"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLM79_17575"
     CDS             complement(<1..240)
                     /locus_tag="PLM79_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88169.1"
                     /translation="METVRTLDWAFGEIEGTRPRFGTVPRPLSWEASRSLALLGQLIG
                     GVRPPLSGPELHRLAMALLSDLPLPDEELLSRLRDR"
     gene            450..1493
                     /locus_tag="PLM79_17580"
     CDS             450..1493
                     /locus_tag="PLM79_17580"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOV88170.1"
                     /translation="MKLGIIGLGRSGKTTVFNALTQRTGESVPPGGHAVPVLGTVSVP
                     DARVDWLSGLYRPKKTTYAQVTYMDLQGTPGSMEDKKEYMSLLLTHMRPMEAFLVVVR
                     NFPDPTLGAPDAARELREIEEEFLLADLATVEKRLERIAAELKKGRKDASREKELLEA
                     CGEVLNRERPLRSRQDLADAPELRGFTFLSAKPRLVIVNNPDEEEALPPLPLGEEEAL
                     VVRGKLEMEMAQLSDEEAAGFREEFGIRETALHRVIERSYRLLRRVTFLTVGEDEVRA
                     WTIPDRLPAVEAAGVVHSDIQRGFIRAEVVAYRDLHRCGDYAAARKQGLVRLEGKTYP
                     VEDGDVIYFRFNV"
     gene            complement(1569..2966)
                     /locus_tag="PLM79_17585"
     CDS             complement(1569..2966)
                     /locus_tag="PLM79_17585"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HOV88171.1"
                     /translation="MKGRREGNQELDPMTAPASIPSPEPEAFRLPWSGPPVGLFWDQS
                     LVWGLLCVETLQRLRVPFRLLSARDIGRGALEGRSLLLVPGGWAAHKVRALGEVGRER
                     IRAFVEGGGSYLGFCGGAGLALSSPPALSLVPLERMPLGERLPSASGEVWIEGLPHHP
                     AWENLPPVLPTSVWWPSQFWGYPLPRCLCLATYRFPGRDFRVADMAVSDVTDRDMVWE
                     EWEKIYGINLDPRRFLGHPALLEVRVGRGRLLLSYPHLETPGDPWGNRLFANLLTYLD
                     ARGRAAAGAGELPRRVEPPEAPSPGEEARRVLAGALDQIEDLICFGERNLLWKWRNSW
                     LLSWQRGIRGLEYGSLAVCLRALEGSVQGMSGSPVRPDPWLEPLQALEAALKEFCPSA
                     RKLLLEEKAAMNSGRVSKLGQVNRTVDALRNRLFGPQMNHGGLCAEIFNRLDRLLMDA
                     LGGPASPAGWFPKMP"
     gene            complement(2963..>4438)
                     /locus_tag="PLM79_17590"
     CDS             complement(2963..>4438)
                     /locus_tag="PLM79_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOV88172.1"
                     /translation="RTRATAIDRRNKTVHLVDLATGKETDLPYDHLVLATGSRPNSLP
                     VPGADLPGVMSLGNLRSAIQIKDLIARGKVGKAVIIGAGAIGCEMAEALADLWGIETS
                     VVEIANQVLPSILDANLARLVQKHMEEKEVAVYLGETVREIRPGDGEHSLSVVTSQRS
                     LEADLVISAVGFRPNSELARSAGLLVSPRGAVVVNGRLQTSDPDIYAGGDCIEVLHLV
                     TGKPVFFPQGSLANRQGRIIGTNLAGGNAVFPGVVGSFILKVFDLAVCSTGLTLEGAT
                     REGFRAVHALVVQADRAHFYPDQDLLYLQLVVDAKTRRILGAQGVGHNGDALAGRINP
                     IAAALPYRPTLEDVSNLELAYSPPFASALDILNAAANTAENILDGYNKPMDPDEFERC
                     FLEEKQEDTLCLDIRSAANAAPFVRRFGERWLNIPQETLKVRMAEVPREKRLIVVCNA
                     GGRSYEALRQLETAGECRAVNLQGGIAVLKKAGILDSNEDE"
BASE COUNT          797 a         1374 c         1502 g          765 t
ORIGIN      
        1 ccggtcccgc agccgggaaa gcaattcctc gtcgggcagg ggcaaatcgg acagcagggc
       61 catcgccagc cggtgcaact cggggcccga caggggtggg cgaacccccc cgatcagttg
      121 acccaggagg gcaagcgaac gggaggcttc ccaggaaaga gggcggggga cggtcccgaa
      181 cctgggccgg gttccctcga tttccccaaa cgcccaatca agggtgcgaa ccgtttccat
      241 gatgggtcct cccggttgcc gggcgtctcg acggagagcc cggcgcaggc gtggttggaa
      301 accattggat cgaaccttac gaaacccggt cgccttctgt caagttccgc gggaatcgag
      361 gattatcctg ttgtcccaat gccccggggg gattaggatg agaccccgat tgggaaggcg
      421 tcggtttccg ttcaagaaag gtgagtggga tgaagctggg aatcatcggg ttgggaaggt
      481 cggggaagac taccgttttc aatgccttga cgcagcgcac gggggaatcg gtcccgccgg
      541 gagggcacgc cgttcccgtc ctgggaacgg tttcggtgcc cgacgcccgg gtggattggc
      601 tgagcgggct gtaccggccc aagaaaacca cctacgccca ggtcacctac atggatctcc
      661 aggggacccc cggttccatg gaagacaaga aggagtacat gagtcttctt ctcacgcaca
      721 tgcgccccat ggaagccttc ctcgtggtgg ttcgcaactt tcccgacccc accctggggg
      781 caccggatgc ggcaagggag ctccgggaga tcgaggagga attcctgctg gcggacctcg
      841 ccaccgtgga aaagcgactc gaacgcatcg ccgcggagct gaaaaagggg agaaaggacg
      901 cgtcccgtga gaaggagctc ctcgaggcct gtggggaagt attgaaccgg gaaaggcctt
      961 tgcgctcccg gcaggacttg gccgacgccc ccgagttgcg cgggtttacc tttctttcgg
     1021 ccaagccgcg gctggtcatc gtgaacaatc cggacgagga ggaggccctg ccgcctctgc
     1081 ccctgggcga agaagaggcc ctggtggtcc gggggaaact cgagatggag atggctcaat
     1141 tgtccgacga ggaagcggcg gggttccggg aagagttcgg catccgggag accgcgctcc
     1201 accgggtgat cgagcggtct tatcgcctgc tgcgccgggt gaccttcttg acggtgggag
     1261 aggacgaagt ccgggcgtgg accatccccg accgccttcc ggccgtcgag gcggcggggg
     1321 tggtgcattc ggacatccaa agggggttta tccgggcgga agttgtggcc taccgagacc
     1381 ttcaccggtg cggagactat gcggccgccc gcaaacaagg gttggtgcgc ctcgaaggca
     1441 agacctaccc cgtggaagac ggcgacgtca tctactttcg attcaatgtt tgatgatccc
     1501 gcaaaaagtc gtttttcgaa accggaaccg aatgaattca gtcggttagg gccggagagg
     1561 cgcacttttc aaggcatttt gggaaaccat cctgccggag aggccgggcc gcctagggcg
     1621 tccatcagca gacggtccag ccggttgaag atctcggcgc acaggccgcc gtgattcatt
     1681 tggggaccga agaggcggtt cctgagcgca tccaccgttc ggttgacttg tcccagcttg
     1741 ctcacccggc ccgaattcat ggcggctttc tcttcgagaa ggagctttcg agccgaggga
     1801 cagaattcct tcaaggctgc ttccagggcc tggaggggtt ccagccacgg gtcgggacgg
     1861 accggggagc ccgacatccc ttggacggag ccttcaaggg cgcgcaggca cacggccagg
     1921 gagccgtatt cgagaccgcg aatgccccgc tgccagctga gaagccagga gttgcgccat
     1981 ttccacaaga gattgcgttc cccgaaacat atgaggtcct cgatctgatc gagggcgcct
     2041 gccaggaccc ggcgggcttc ttcgcccggc gaaggggcct cggggggctc gacgcgccgg
     2101 ggtagttccc ctgcgcccgc cgccgccctt ccccgggcgt cgaggtacgt gagcaggttg
     2161 gcaaagagcc gattgcccca aggatctccc ggggtttcca ggtggggata ggacaggagg
     2221 agtcgtcctc gacccacgcg aacctcgagg agggcgggat ggcccaggaa ccttctcggg
     2281 tccaggttga tcccgtagat cttctcccat tcttcccata ccatgtcccg gtccgtgacg
     2341 tccgagaccg ccatgtcggc cacccggaaa tcccgacccg gaaaccggta cgtggcaagg
     2401 cagaggcaac gggggagggg atatccccaa aattgggaag gccaccatac ggaggtggga
     2461 agaacgggcg ggaggttttc ccacgcgggg tggtgcggaa ggccttcgat ccagacttcc
     2521 ccgctggcgc tgggcaggcg ttccccgagg ggcattcgtt ccaggggaac cagggacagg
     2581 gcgggggggc tcgagagggc caggcccgcg cctccgcaaa atcccaggta gctgccgccc
     2641 ccctccacga aggctcgaat ccgttcccgg ccgacctctc ccagggcccg gaccttgtgg
     2701 gccgcccacc cccccggcac caggagcagg gagcggccct cgagcgcgcc ccggccgatg
     2761 tcccgggcgc tgagaaggcg gaaagggacc cggagccgct gcagggtttc cacgcacagg
     2821 aggccccaaa ccagggattg gtcccagaaa aggcccaccg gggggccgct ccagggaagg
     2881 cggaaagcct cgggttcggg agagggaatg gatgcgggtg cggtcatggg atcgagttcc
     2941 tggttcccct cccttctccc cttcattcgt cttcgttcga atccaggatc ccggcttttt
     3001 tcaggacggc gattcccccc tggaggttga ccgcccggca ttcccccgcc gtttcgagct
     3061 gtcgcaaggc ttcgtaggac cgtcctcccg cgttgcagac cacgatgagg cgtttttccc
     3121 gggggacctc ggccatgcga accttgaggg tttcctgggg gatgttcagc cagcgttcgc
     3181 cgaatcgccg gacgaagggg gccgcattgg ccgcggaccg gatgtccaga cagagggtgt
     3241 cttcttgctt ttcctcgaga aaacaccgct cgaactcgtc cgggtccatg ggcttgttgt
     3301 acccgtcaag gatattctcc gccgtattgg cggcggcgtt caggatgtcc agggccgagg
     3361 cgaagggcgg ggaataggcc agctcgaggt tcgagacgtc ctcgagggtg ggccgatagg
     3421 gaagggcggc ggcaatggga ttgattcttc ccgccagggc gtctccgttg tgcccgactc
     3481 cctgggcccc caggatgcgg cgggtcttgg cgtccaccac gagctgcagg tacagcaggt
     3541 cctggtccgg gtagaagtgg gcccggtccg cctggaccac caaggcgtgc acggcccgga
     3601 atccctcccg cgttgccccc tcgagggtga gacccgtaga gcacaccgcc aggtcgaaga
     3661 ccttgaggat gaaactgccc accactcccg gaaagacggc gttgcctccc gccaggttgg
     3721 tgccgatgat gcggccctgg cggttggcca gggaaccctg gggaaagaag accggttttc
     3781 ccgtcaccag gtgaaggact tcgatacagt ctcctcccgc gtaaatatcc gggtccgagg
     3841 tctggagtcg cccgttgacc acgacggctc cccgggggga cacgagcagc ccggcggacc
     3901 gggcgagctc cgagttggga cgaaacccga cggcggaaat caccaggtct gcctccaggc
     3961 tcctttggga cgtgaccacg gacagggaat gttctccgtc ccccgggcgg atctcccgga
     4021 cggtctcccc caggtacacc gccacttcct tctcttccat gtgcttctga acgagccgag
     4081 ccaggttggc gtccaggatg gagggaagga cctggttggc gatctcgacc acgctggtct
     4141 cgattcccca caggtcggcc agggcttcgg ccatttcaca cccgatggcg cccgctccga
     4201 tgatgaccgc ctttcccacc tttccccgag cgatgaggtc cttgatttgg atggcggatc
     4261 gcaggttccc gaggctcatc actcccggca ggtcggctcc cggcaccggc agggagttgg
     4321 gacggctccc cgtagccagg accaggtggt cgtaaggcag gtccgtctcc ttgcccgtgg
     4381 ccaggtccac gaggtgaacc gtcttgttgc gccgatcaat ggccgtggcc cgggtgcg
//
LOCUS       DAOOVC010000240         4416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10891_length_4416_cov_6.165329, whole genome shotgun sequence.
ACCESSION   DAOOVC010000240 DAOOVC010000000
VERSION     DAOOVC010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10891_length_4416_cov_6.165329"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..436)
                     /locus_tag="PLM79_17595"
     CDS             complement(74..436)
                     /locus_tag="PLM79_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88173.1"
                     /translation="MPKILVVDDEEHIRLLYSEELKESGYEVITAQSGYKLLERIEEE
                     QPDLVILDIKMVDYNGLDLLQDIRNHYHDLPVILSTAYDTFKEDMKSIAADFYVVKSF
                     DLTELKKKIAVALEARLP"
     gene            complement(558..1766)
                     /locus_tag="PLM79_17600"
     CDS             complement(558..1766)
                     /locus_tag="PLM79_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOV88174.1"
                     /translation="MPFYEENPNRISQADIVVGIPSYHEAEAIGFTTEQVAKGLTKYF
                     GDLSSVIINCDNQSHDGTKEAFFSADCRVPRIYLSTEGGVRGKGRNVRNLLEKARELG
                     ARVVAEVEADIRNLSPRWIQNLVQPILNGAGYVSPIYVRHKYEATLTTSIIYPLTRCL
                     YGRRVRQPSSGDFGLRGDLVDSYLQTDVWTESVEQFGIDVWMTTIALNARVPICQSVM
                     GSPKLHRVKDPYAQLSVVFRQIVSTIFDLMQIYEPFWRRVKWSKPTILFGVDNVEVET
                     PIPVEVNVNRLHERFLEGFDQYSKTWEAVYGSAVSNKLQEIRSLGLQHFSLPSQTWAR
                     ILFDTARAYKNVGAQERTGLLDALLPLYLGKVLTFVKKTERMSIQQTEEYVENECMIF
                     EENKPYLATM"
     gene            complement(1783..2778)
                     /gene="hflC"
                     /locus_tag="PLM79_17605"
     CDS             complement(1783..2778)
                     /gene="hflC"
                     /locus_tag="PLM79_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOV88175.1"
                     /translation="MMIKGMHAILPIFLAVALFLSGALFVVNENEQVVLTQFGRPVGK
                     PITQAGLHFKIPMIQKANYFEKRIMKWDGNPNQIPTRDKKYIWVDSTARWRIQDPLLF
                     LQRVGSISIAHSRLDGIIDSVVRDHISNNDLVELVRSEGWEDARQKLLDAGIPDIQFM
                     DSRGDLPYGEEISTRLTKGREKITREMVADAARLLPEFGIELLDIRIKRINYIESVQQ
                     KVFERMISERKRIAAQYRSEGEGERAAILGQMERELAKITSEAFRTAQEIRGKADAEA
                     TRIYAEAYGRDPDFYEFYRTMEYYKNSDNSRSSFVLSTETDLFKYLKDAGKVPGK"
     gene            complement(2775..3842)
                     /gene="hflK"
                     /locus_tag="PLM79_17610"
     CDS             complement(2775..3842)
                     /gene="hflK"
                     /locus_tag="PLM79_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOV88176.1"
                     /translation="MDWERPPKRNPKMGNVQDLEEILRQIRSRFPRFFSSNSPVWLIG
                     AVALVVLVAMNSYYMVEPQETAVVLRFGKFIHTAESGLHFKVPFGIDSVRKVVTGRVL
                     QREYGYRTAQPGIRSRFVEKGHEEEAVLLSGDLNVLNFQWTVQYKIQDPVDFLFRLQD
                     VDGTLDDISESVIRRIVGNRYSDEVLTVGRASIGDQAMVEIQKIMDSYRAGIQILTVK
                     LQNVNPPDPVKTAFNEVNEARQEQERMINEAQQAYNQKIPRAAGEARQTVTQAEGYAL
                     ERVNRSMGETSRFLEILAEYRKAPEVTRRRMYLESVPELLGRVDRLYILDENERGLLP
                     LFNLNPAASGTGKGSKEGEGR"
     gene            complement(3982..4209)
                     /locus_tag="PLM79_17615"
     CDS             complement(3982..4209)
                     /locus_tag="PLM79_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88177.1"
                     /translation="MRANGRWGWVDVEENDRGQGMRVRVLILRSALGLAFAYLLIRWF
                     FPAAGIVTILAAAALLVFFAYLFESVRPGNQ"
BASE COUNT          842 a         1351 c         1205 g         1018 t
ORIGIN      
        1 agcaacccca agcagtgagg cccgaccgtt cccggcccga aagacccgcg cggcatgccc
       61 ggccgacgga aaatcaaggc aggcgtgctt ccagggccac ggcaatcttc tttttgagct
      121 ccgtcaaatc gaagctcttg accacataga agtccgccgc gatagacttc atgtcctctt
      181 tgaatgtgtc gtaggccgtg gacagaatca cgggcagatc gtgatagtgg ttgcggatgt
      241 cctggagcag atcaagaccg ttgtagtcca ccattttgat atcgagaatc accaagtcgg
      301 gctgttcttc ttcaatccgt tccaggagct tatagccgct ttgggccgtg atcacttcat
      361 aacccgactc tttgagctct tcggaataca gtagccgaat atgttcttcg tcatctacaa
      421 ccaagatctt gggcatctct tcctctcctt gatcctcttg gaatcgcgaa tcgcggtcgg
      481 acccctgtcg agggttcgtt gcttcttcgg gtgcggcggg tttccccggg ccatcctcct
      541 tttgccgttg acggcgctta cattgtggca aggtagggtt tgttttcctc aaaaatcatg
      601 cactcgtttt ccacgtattc ttccgtctgc tggatggaca tccgttcggt ctttttcacg
      661 aaagtcagga ccttgcccag gtagaggggc aggagtgcgt ccaggagtcc cgtccgttct
      721 tgcgccccca cattcttgta ggccctggcc gtatcgaaca ggatcctagc ccatgtttgg
      781 ctgggaaggg aaaagtgttg gagccccagg cttcggattt cctggagctt gttggacacc
      841 gcgctcccgt acaccgcctc ccaggtcttg ctgtactgat cgaaaccttc gaggaaacgc
      901 tcatggagcc gattcacgtt cacctccacc gggatggggg tttccacctc cacgttgtcc
      961 actccgaaaa ggatcgtggg cttactccat ttcacccggc gccagaaggg ctcatagatc
     1021 tgcatgagat caaagatggt gctcacgatc tgccggaaaa ccaccgaaag ctgggcgtac
     1081 gggtccttca cccggtgtag ttttggagaa cccataacgg actggcagat gggcacccgg
     1141 gcgttgaggg caatggtggt catccagaca tcgatgccga actgctccac cgattcggtc
     1201 catacgtccg tttggaggta ggaatccacc aggtcgcccc tgagcccgaa gtcaccggaa
     1261 ctgggctgtc ggactcgacg accgtagagg caccgggtga ggggatagat gatggaggtg
     1321 gtgagagtgg cttcgtactt gtgccgcacg tagatgggcg acacgtaccc tgcgccgttc
     1381 aggatcggct gaaccaggtt ctgaatccag cgcggcgaca ggtttctgat gtcggcctcg
     1441 acctctgcaa ccacccgggc acccagttcc cgggctttct cgagaagatt tcgaacgttc
     1501 cttcccttgc cccgcacgcc cccctccgtg gaaaggtaga tgcggggaac cctgcagtcc
     1561 gcggagaaga aggcttcctt ggtgccgtcg tgggactggt tgtcgcagtt gatgatcacg
     1621 ctgctcaggt ccccgaagta tttcgtcaat cccttggcga cctgttccgt cgtaaacccg
     1681 atggcttccg cttcatgata ggagggaatg cccacaacaa tgtcggcctg ggagattcga
     1741 ttcgggtttt cctcgtaaaa cggcatagac actcctcctc cttcacttcc cggggacctt
     1801 tcccgcatcc ttcaggtact tgaacagatc ggtttccgtg gacaaaacga aggagctcct
     1861 cgagttgtcg gaattcttat agtactccat ggtccggtag aactcataaa aatccgggtc
     1921 tctgccgtag gcttccgcgt aaatccgggt ggcttcggca tccgctttgc cccggatttc
     1981 ctgggccgtc cggaacgctt ccgaagtgat cttggccagc tcccgttcca tctgccccag
     2041 gattgcggcc cgttcccctt ccccctccga gcggtactgg gcggcgatcc tttttcgctc
     2101 ggagatcatc cgttcgaaca ccttctgttg cacggattcg atgtagttga tgcgcttgat
     2161 gcggatgtcc aggagctcga tgccgaactc gggcaggagt cgtgcggcgt cggccaccat
     2221 ttcccgggtg atcttctccc gccccttggt caggcgggta ctgatctcct ccccgtaggg
     2281 aagatctccc cgggaatcca tgaactggat gtcggggatg ccggcgtcca ggagcttctg
     2341 gcgggcgtct tcccagcctt cgcttcgcac cagttccacc aggtcgttgt tggaaatgtg
     2401 gtctcgaacc acggaatcga tgatcccgtc caggcggctg tgggcaatgg aaatgcttcc
     2461 cacccgctgg aggaaaagca ggggatcttg gatccgccac cgggccgtcg agtccaccca
     2521 gatgtatttc ttgtctctgg tgggaatctg gttgggattc ccgtcccatt tcatgatccg
     2581 cttctcgaag taattggcct tttggatcat ggggattttg aaatggaggc cggcttgggt
     2641 gatgggcttt cccacgggtc tcccgaattg agtcagcacg acctgctcat tttcattaac
     2701 cacaaaaaga gcgccggaca ggaacagcgc cacggcaaga aagatgggga ggattgcatg
     2761 catgcccttg atcatcatcg cccttccccc tccttcgacc ctttcccggt gccggaagcg
     2821 gccggattca agttaaacag ggggaggagc cctcgttcat tctcatccag gatgtagaga
     2881 cgatccaccc ggcccagcaa ctcggggacg gactcgaggt acatgcgcct gcgggtgacc
     2941 tcgggagcct tccggtattc ggcaaggatc tcgaggaaac ggctggtttc gcccatgctc
     3001 cggttcaccc gctcgagggc gtatccttcc gcctgggtca cggtctgtcg agcttccccc
     3061 gcggctcgag ggatcttttg attataagcc tgctgggcct cgttgatcat ccgctcctgt
     3121 tcctggcggg cctcgttcac ctcgttaaag gccgttttga cgggatccgg cgggttcacg
     3181 ttctgcagct tgaccgtaag gatctgaatt cccgcccggt agctgtccat gatcttttgg
     3241 atttcgacca tggcctggtc gccgatggat gcacggccca cggtgaggac ctcgtctgaa
     3301 taccggttgc cgacgatccg acgaatgacg gattccgaaa tgtcgtccag ggtgccgtcc
     3361 acatcctgca accggaaaag aaaatccacg gggtcctgga tcttgtactg gacggtccac
     3421 tgaaagttca agacgttcag gtccccgctc aacagcaccg cttcctcttc gtgtcccttt
     3481 tccacgaacc gggaacggat tcccggctgg gcggttcgat agccgtattc cctttgcagg
     3541 acccgcccgg tcaccacttt gcgcacggaa tcaatcccaa acgggacctt gaagtggagt
     3601 cccgattccg ccgtgtggat gaacttaccg aaacgcagta ccactgcggt ttcctggggc
     3661 tcgaccatgt aataggaatt catcgcgacc aggacgacca acgcgacggc cccgatcagc
     3721 cacaccgggc tgttgctcga gaagaagcgc ggaaagcggc ttctgatctg ccgcaagatc
     3781 tcctccaggt cctgcacgtt gcccatcttc gggtttcttt tgggcggcct ttcccaatcc
     3841 attcattctc ctcattgctg caacgaccta ccacacccct gccgggagtc gctccctcgc
     3901 aaagcgaacc tcctccccgc gacaccgggc ctccggccga ggcttttcgc ctctcctcgg
     3961 cggtcgccgg caaaaccatc attattgatt ccctggacga accgattcga atagataggc
     4021 aaaaaaaacc agcagcgccg ccgccgccag gatggtcacg attcccgccg cggggaagaa
     4081 ccagcgaatc aggaggtagg caaaggccaa accgagcgcc gaccgaagaa tcagcacccg
     4141 aaccctcatt ccctgcccac gatcattttc ttccacgtcc acccatcccc atcgtccgtt
     4201 tgcccgcatt tgccgaatcc tcccccgacc ggacccgcat atctcgacgc atcctactaa
     4261 tgcccatata tcttgtcaag cttgcgaggc gccgatcctt tcggcttcgt tccggtttcc
     4321 gcccgaaccc ggactattgc caaaaacgcc ggaatgggca tatattctct tcctgttcat
     4381 tttgcgggct cgcgccgttt cggggtcgat tcatcg
//
LOCUS       DAOOVC010000241         4415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_10895_length_4415_cov_5.241972, whole genome shotgun sequence.
ACCESSION   DAOOVC010000241 DAOOVC010000000
VERSION     DAOOVC010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10895_length_4415_cov_5.241972"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PLM79_17620"
     CDS             <1..639
                     /locus_tag="PLM79_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88178.1"
                     /translation="PWVEISVKDTGQGIPEEIRERIFDPYFTTKKKGQGTGLGLAVVR
                     GIVQDCNGHLSVESKTGAGSTFRVRIPRTDGEVVSPAGPSLEPPSRKASILFVDDEPP
                     IAQVCRRLLTAMGFRVTAMTDSREAVELFRKNPDSYDLVITDLVMPSMTGMELIRKIR
                     KTRKDIPVVLCTGYSEKTCDEEMRDLEIGDFLLKPLNMEKLLTSIHRVLRKP"
     gene            818..1006
                     /locus_tag="PLM79_17625"
     CDS             818..1006
                     /locus_tag="PLM79_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88179.1"
                     /translation="MSLNETRNSEGRTPMSMIFTAKFRSRQPEMHSTEIAKEKVTAVK
                     ALKTGDTFELESKTAGIE"
     gene            complement(1060..2358)
                     /locus_tag="PLM79_17630"
     CDS             complement(1060..2358)
                     /locus_tag="PLM79_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HOV88180.1"
                     /translation="MPVWNEREECLGLEERVQQQWERLQSSLNRAHKNVPFYRNHFRE
                     EKIDPAQMETLSDWERIPFTFPRHFGENYPYGLFAVPLRDVVRIHTARGIELKPIVSG
                     YTKQDIGIWREIMARALTAAGVSRADILQIDLDPGLANWGRHYKDGAESIEAGVIPLT
                     LLPHEKQLMVLRDYKTSVLITSTSAALQLKDLLFRANLNPNSLSLKTLILVGEPTGAA
                     VQRRIEEELHVRTWVHYGLSEVPGPGLAFQCEERQGLHVSEDHFFPEVVDPETGRSVP
                     EESEGELVLTTLTTRAFPLIRFRTGDRVRIFSKPCPCGRTLRRMEWLPHRTDEMMIIR
                     GVKVHHRQVLLLLSRFLGFSPHPHRFLVRRREFIDTLEVWLKVDEQVFSDEIKEMEKL
                     CQRLEAELAQELGVPVKIRLKEEDSFSNVGSNGAIEDLRP"
     gene            complement(2432..3175)
                     /locus_tag="PLM79_17635"
     CDS             complement(2432..3175)
                     /locus_tag="PLM79_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88181.1"
                     /translation="MLRIRNLRCHYGRIRAINGVSLSVRPGELIALIGANGAGKSTLL
                     QSICGLHGQWEGEILFQKEPLKGLKPPAIVGMGVSLVPEGRMLFPPMSVLDNLRLGAY
                     VRYRRRENREIARDLDSVFELFPVLRERAQQPAGTLSGGEQQMLAMGRALMARPRMLL
                     LDEPSMGLAPFIVRLILDILLQLKEQGMTILLVEQNARAALQIADRGYVIENGRIVLD
                     GPAGELLVDQDVKRAYLGKDYREFCEGRT"
     gene            complement(3180..3974)
                     /locus_tag="PLM79_17640"
     CDS             complement(3180..3974)
                     /locus_tag="PLM79_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88182.1"
                     /translation="MRDRETDEKAVPPLLKIEGISKSFGGIQALYDVSFEVFPETIHA
                     IIGPNGAGKTTLFNIITGVSAPDAGAIRYGPRNIVGKQVHELVAQGIARTFQNVELFG
                     SMSAMENVLVGRHVRTACGFVGAMGRWSWVRTEEEEARQKAMDLLRFVGLDSVAHTRS
                     SDLPFGWQRFLEIARALATEPRVLLLDEPASGLNAVETQRLGELLQEIRGRGVTLLLV
                     EHDMSLTMGVSDRILVLHQGKRLAEGTPREIQEDDAVICAYLGKGI"
     gene            complement(3928..>4415)
                     /locus_tag="PLM79_17645"
     CDS             complement(3928..>4415)
                     /locus_tag="PLM79_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV88183.1"
                     /translation="LVWGTVFVSFILCERIVRSRVGRALRAIHDSEKAAAAMGIDTSR
                     LKISVFVLSALFAALAGFLYAHLVSFVSPSSFDFLVSVRIVTMVVIGGMASIWGSLLG
                     ASLLTVLPDWLHVFSDYEMLVYGLVLMVVMIFLPQGLTRGILDLYERSRNRREGRSPS
                     A"
BASE COUNT          896 a         1370 c         1330 g          819 t
ORIGIN      
        1 ccttgggtcg agatttccgt caaggacacg gggcagggga ttcccgagga aatccgggag
       61 cggatcttcg atccctactt caccaccaag aagaaaggac aggggacggg gctgggattg
      121 gccgtggtcc gcggaatcgt tcaagactgc aacgggcacc tgtcggtgga gagcaaaacg
      181 ggtgcgggct cgaccttccg agtgaggatt cctcgaaccg acggagaagt ggtttcccct
      241 gccggtcctt ccctcgagcc cccttcgagg aaggcaagca ttcttttcgt ggatgacgag
      301 ccgcccattg cccaggtgtg ccgccgactc ttgacagcca tgggatttcg ggtgaccgcg
      361 atgaccgaca gccgcgaggc cgtggagctc ttccgaaaga atccggattc ctatgacctg
      421 gtgatcacgg acctggttat gccgagcatg acggggatgg aactgatccg aaaaatccgc
      481 aagacccgga aagacatccc ggtggtgctc tgcacgggct acagtgagaa gacctgcgac
      541 gaagaaatgc gggacctgga aatcggggac tttttgttga agcctctaaa catggagaaa
      601 ttgctgacat ccatccatcg tgtgttgcgc aagccctgat gccagttgcc aaggtcgctc
      661 cgtcatgctg agcgaaaatc tcaagcaaac ggctgagtac tgcgggttgg cagtttcggc
      721 ttgagtgcag ctttgtctgc cccccatgac ttccccaaac ggcgggcggg gccggtgctt
      781 gtgagaagcg cgcttgtcga ggaggtcggc ccgtccagtg agcctcaacg agactcgaaa
      841 cagcgagggg agaactccca tgtccatgat ctttacggcg aaattccgga gccggcagcc
      901 ggagatgcac tcgacggaaa tcgccaagga gaaggtcact gccgtcaagg ccctcaagac
      961 cggggacact ttcgagctcg aaagcaagac cgcgggtatt gaatgagggc ggaagatttt
     1021 ccccggtgcg aaagctcccg ggcgaatcgg ccgcgggtcc tagggtcgca ggtcctcgat
     1081 ggccccattg gaacccacat ttgaaaaaga atcctcttcc ttgagccgga tcttgacggg
     1141 gactcccaat tcctgagcca gttcggcctc gagcctctgg cagagcttct ccatctcctt
     1201 gatctcatcg gagaagacct gctcgtccac cttcaaccag acctccaggg tgtcgatgaa
     1261 ttccctcctg cggacgagaa accgatgagg atgggggcta aaacccagga atcggctgag
     1321 gagcaggagc acctggcgat gatggacctt gaccccccgg atgatcatca tctcgtcggt
     1381 tcgatgggga agccattcca tgcgccgcaa ggtcctgccg caggggcagg gtttggaaaa
     1441 gatgcgaacc cggtccccgg tgcggaaccg aattagggga aaggcacggg tggtgagggt
     1501 ggtcagtacc agctcccctt cgctttcttc ggggacactc cgcccggtct ccgggtccac
     1561 cacctcggga aagaagtgat cttcactcac gtggaggccc tgtcgttcct cgcactggaa
     1621 cgccagcccg ggccccggga cctcgctcaa cccgtagtgg acccaggttc ggacgtggag
     1681 ctcctcctcg atcctccgct ggacggccgc accggtgggc tcccccacca ggatgagggt
     1741 ctttagagac agggaattgg gattcaggtt ggcccggaaa aggaggtctt tgagctgcag
     1801 ggctgcggag gtgctggtga tcaggaccga ggtcttgtag tccctcaaca ccatcaactg
     1861 cttttcgtgg ggcaacaagg tcaggggaat cacgccggct tcgatgcttt cggccccgtc
     1921 cttgtagtgg cggccccagt tggccagtcc cggatcgagg tcgatctgga gaatgtccgc
     1981 ccgggacacc ccggccgcgg tgagtgcccg ggccatgatc tcccgccata tcccgatgtc
     2041 ctgcttggtg taaccgctca cgatgggctt cagctcgatg ccccgggccg tgtggatgcg
     2101 caccacgtcg cgcaggggca cggcaaacaa cccgtaggga taattctctc cgaagtgccg
     2161 ggggaaggtg aacggaatcc gctcccagtc ggaaagggtt tccatctgag cagggtcgat
     2221 cttctcttcc cggaaatgat tccggtaaaa cggcacgttc ttgtgggcgc ggttcaggga
     2281 gctctggagc cgctcccatt gctgctgcac cctttcctcc aggcccaggc actcttctcg
     2341 ctcgttccaa acgggcatgg ttgccccctc ccatcccaaa tcacctgcaa gtctcgaggc
     2401 ccggatcctt cccgacgccc ctcccctgct ctcaggtcct cccttcgcaa aattcccgat
     2461 aatccttacc caggtaagcg cgcttcacgt cctggtccac caagagctcc cccgcgggac
     2521 cgtccaggac gattcgaccg ttttcaatca cgtatccccg gtccgcgatt tgcaaagccg
     2581 cccgggcgtt ctgttccacc aggagaatgg tcatgccctg ctctttcaac tggagaagga
     2641 tatccaggat gagccgaacg atgaaagggg ccagccccat ggacggctcg tccaggagaa
     2701 gcattcgagg acgggccatg agggcccgcc ccatggccag catctgctgt tctcccccgg
     2761 aaagggtccc cgccggttgt tgagcgcgct cccgcaacac gggaaacaat tcgaacaccg
     2821 aatccaggtc ccgggcgatc tcccggttct cccggcggcg gtaacgcacg taggcgccca
     2881 accgaagatt gtccaaaacg ctcatggggg ggaaaagcat ccgcccttcg ggcaccaggc
     2941 tgactcccat tcccacaatg gccgggggct tgagaccctt gagaggttcc ttctgaaaaa
     3001 ggatctcccc ctcccactga ccgtggagac cgcaaatgga ctgaagcagg gtgcttttac
     3061 ccgctccgtt ggcgccgatg agggcgatga gttcccccgg ccgaacggag aggctcaccc
     3121 cgttgatggc ccggatgcgg ccgtagtggc atcgaaggtt gcggatccgc agcattggat
     3181 cagatcccct ttcccaggta cgcgcaaatc accgcatcgt cctcctggat ttcccggggc
     3241 gttccttcgg ccagcctttt gccctgatga aggacgagaa tgcggtccga gactcccatg
     3301 gtcagggaca tgtcgtgctc gacgaggagc agggtcactc cacggcctcg aatctcctgg
     3361 agcagctctc cgagccgctg ggtttccacg gcgttgaggc ccgaggccgg ttcatccagg
     3421 agaagcaccc ggggctccgt ggccagtgcc cgggcaatct cgagaaaccg ctgccagccg
     3481 aacgggagat cggaggaccg ggtatgagcc acggaatcga gtcccacgaa gcggaggaga
     3541 tccatggctt tttggcgggc ctcctcttct tcggtgcgca cccaggacca ccgacccatg
     3601 gcccccacaa acccgcaggc cgtcctcacg tgtcggccca ccagcacgtt ttccatcgcg
     3661 gacatgctcc cgaacagctc gacgttctgg aaagttcgag cgatcccttg ggccacgagc
     3721 tcatggacct gcttcccgac gatgtttcgg gggccgtacc ggatggcgcc cgcatcggga
     3781 gcgctcaccc ccgtgatgat gttgaacagg gtggtctttc cggcgccgtt ggggccgatg
     3841 atcgcatgaa tcgtttccgg gaacacctcg aaggagacgt cgtagagcgc ctggattccc
     3901 ccgaagctct tggagattcc ctcgatttta agcagagggg gaacggcctt ctcgtcggtt
     3961 tctcgatctc tcatacaagt ccagtatccc ccgggtaaga ccctggggca gaaaaatcat
     4021 gaccaccatc agcaccagcc cgtaaaccag catctcgtag tcggaaaaga catggagcca
     4081 gtcgggaagg accgtgagca gggaggcacc cagcagggag ccccagatgc tggccattcc
     4141 cccgatgacc accatggtca cgattctcac cgaaaccagg aaatcgaagg agctggggct
     4201 gacgaagctc accaggtggg catacaggaa tcccgcgagg gcggcaaaga gggcgctgag
     4261 gacgaagacg ctgatcttga gccgactcgt gtcgattccc atggccgcag ccgccttttc
     4321 gctgtcatga atggcgcgaa gggcccgccc caccctcgac cggacgatcc gctcacacag
     4381 gatgaagctc acaaacaccg tcccccacac cagga
//
LOCUS       DAOOVC010000242         4382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_11013_length_4382_cov_5.855096, whole genome shotgun sequence.
ACCESSION   DAOOVC010000242 DAOOVC010000000
VERSION     DAOOVC010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11013_length_4382_cov_5.855096"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..818)
                     /locus_tag="PLM79_17650"
     CDS             complement(99..818)
                     /locus_tag="PLM79_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88184.1"
                     /translation="MLKVENLHVRYGNIEALHGISFWVNEGEMVALLGANGAGKSTAL
                     MSVMRVAPPEGPVVTRGTITYGGQDLLPFPCHAVVARFGIGLVPEGRHIFGNLTVEEN
                     LKLATYARKDPDGIARDYEQVFSLFPRLADRRRQRGDTLSGGEQQMLAMGRAFMSGVR
                     CILLDEPSMGLAPFLMQELFRVLKQINEKGTTILVVEQNAGIALKYAHRAYVLEAGRI
                     VMEGPSEELSRDTKIRRAYLG"
     gene            complement(811..1587)
                     /locus_tag="PLM79_17655"
     CDS             complement(811..1587)
                     /locus_tag="PLM79_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88185.1"
                     /translation="MPLLEIQNVTLFFGGLRAVYGFSLTLEGGELLGLIGPNGAGKTT
                     VFNLVSGFYRPTDGEILFRGKNIAGLRPHAITALGMARTFQNIRLWNSLTVFENLCVS
                     QHYHLGYGFLDSVLRTRRFRDREHRVERNAEHLLEILNLGHYAREYPKNLPYGLQRRL
                     EIGRALSLQPKLLLLDEPAAGMNPSEADQLIELILWIRKEFHLAVWLIEHHMRVVMNV
                     CEWIRVLDFGETIAEGTPEEVKKNPRVVRAYLGDEEAGHA"
     gene            complement(1588..2625)
                     /locus_tag="PLM79_17660"
     CDS             complement(1588..2625)
                     /locus_tag="PLM79_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV88186.1"
                     /translation="MNPNMRKCISVFCFILPGLLVVAAAGGNWINAYLQLVLMYIGIN
                     IILSASLNLVNGYMGEFSVGHAGFMAVGAYTTSVLNVWLFSQSQVFGPPVLAPSSALY
                     LFPITLLLGGVAAALAGLLVAVPSFRTRGDYLAIITLAVNYIVKSSIENLQVIGGARG
                     FMGMRAIVNSMQEVVNLPWIMIWIVISVGFTLWILRQFVTSTYGKGIVALRDDEIAAE
                     IMSVNTRKMKIVAFMLSSGLAGIAGGLFAHVLGYINPGTFTIMKSTEVLVMVYLGGMG
                     SLSGSVLSAVGLTVLLELLRPLQVIKWVVVPLLLILVMLFRPEGIMGHREFTDVFPRL
                     RRIFSTRGKPV"
     gene            complement(2629..3555)
                     /locus_tag="PLM79_17665"
     CDS             complement(2629..3555)
                     /locus_tag="PLM79_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOV88187.1"
                     /translation="METFLQNLLNALQWGSFYALIALGYTMVYGVLLLINFAHGDIFM
                     VGAYLGLFIAGILLGHHSAVFPFQLPPSMVFGLTILFTMILTAGVGVAIERVAYRPLR
                     RKGASRLYVVITALMCGLILEHGNLALLGANRRSFPEIIEKTVYTVAGITFSNVKVLV
                     VIATVLLFLLLEFLVRKTKLGMAMRAISYDRMAVPLMGIPVDTVIVFTFILGSSMAAV
                     AGVLFAVAYPVLEPYMGALVGWKAFIAAVIGGIGEIRGAFVGGFLLGFVEIFVAAFFP
                     STLRDLISFTILLVFLSIKPMGIFGVARAAKI"
     gene            complement(3653..4372)
                     /locus_tag="PLM79_17670"
     CDS             complement(3653..4372)
                     /locus_tag="PLM79_17670"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV88188.1"
                     /translation="MLCWKALKTCAKLEFKAKKAAVLYDIASDYPKGLAEYFKEAFEK
                     IHGPGSVVAFETFTTKDKDFSAQLTKIVKSGADVLFSPQYYDEVPLIVKQARELGWNK
                     PIMGSDSWGSAELMKLCGDSCKGYHFSTHYAAAGAKGATKDFIDKYHAKYGYVPDDVA
                     ALTWDALGLLVQAMKDTGGFTGDLDKDRNAIQGALVKIKDFEGITGRMTFSAHGDPTK
                     CAVVVKISDKGEFEFFKQACP"
BASE COUNT          907 a         1295 c         1361 g          819 t
ORIGIN      
        1 ggattgttcc tctcttccgt aaccgttggt cgcgggagtt cctctcctct cgggagcctc
       61 gaaggtcccg aagaacgggc tggaacgccc tcgacggttc aacccaggta agccctccgg
      121 atcttggtgt ctcgcgacaa ttcctccgaa ggtccttcca tgacgatcct tccggcttcc
      181 aacacgtagg cgcgatgagc atacttgagg gcgatcccgg cgttttgttc cacgaccagg
      241 atggtggtcc ccttctcatt gatctgcttg agcacccgga acaactcctg catcaggaaa
      301 ggagccaggc ccatggaggg ttcgtccagc aagatgcagc gaacgccgct catgaacgcc
      361 cttcccatgg ccagcatttg ctgctccccg ccgctgaggg tatcgcccct ctgccgtctt
      421 cggtcggcca ggcgggggaa cagggagaag acctgctcgt agtcccgggc gatgccatcc
      481 gggtcctttc gggcgtaggt ggccagtttg aggttttctt ccacggtcag gtttccgaat
      541 atgtgccggc cttcgggaac gagaccgatg ccgaaccggg ccacgacggc atggcaggga
      601 aacggaagta ggtcctgccc tccgtaggtg atcgtgcccc gggtcaccac cggaccctcg
      661 ggaggggcaa cccgcatgac gctcatgagc gccgtggact ttcccgcccc gttggctccc
      721 aggagcgcca ccatttcccc ctcgttgacc cagaaggaga tcccgtggag ggcttcgatg
      781 tttccgtacc gaacgtgcag gttctccacc ttaagcatgg cctgcctctt catcccccag
      841 gtaggcccgg acgacccggg ggttcttctt cacctcttcg ggagtgcctt cggcgatggt
      901 ttcgccgaaa tcgagcaccc ggatccactc gcaaacgttc atcaccaccc gcatgtggtg
      961 ttcgatgagc cagacggcca ggtggaattc cttgcggatc cagaggatga gctcgatgag
     1021 ctgatccgct tcgctgggat tcatcccggc agccggctcg tccaggagga ggagcttggg
     1081 ctgcagggaa agggcccgac cgatctcgag gcgccgctgg aggccgtagg gaaggttctt
     1141 gggatattcc cgggcgtagt gcccgaggtt gaggatctcg aggaggtgct cggcgtttct
     1201 ttccacccgg tgctcccggt ctcgaaaacg ccgggtacgg agcaccgaat ccaggaaccc
     1261 gtagcctagg tggtagtgct gggagacgca caggttttcg aaaaccgtga ggctgttcca
     1321 caggcggatg ttctgaaagg tcctggccat gcccagggcc gtgatggcgt ggggccgcag
     1381 cccggcgatg ttcttgcccc gaaacaggat ttctccgtcc gtgggccggt agaatcccga
     1441 taccaggttg aagacggtgg tttttcccgc cccgttgggt ccgatgagac ccaggagttc
     1501 gccgccctcg agggtcaggc taaaaccgta cacggcccga aggcccccga agaagagggt
     1561 tacgttctgg atctccaata gcggcattta gactggcttt ccccgggttg aaaagatgcg
     1621 gcgcaggcgc gggaaaacat cggtgaattc ccggtgaccc atgattccct cgggacgaaa
     1681 cagcatgacg agaatgagca ggaggggcac caccacccac ttgataacct gaagaggccg
     1741 aaggagctcg aggagcaccg tgaggccgac ggcgctcagc acggacccgc tcaacgatcc
     1801 catccccccg agatagacca tcaccaggac ctcggtggac ttcatgatgg tgaaggttcc
     1861 gggattgatg tagcccagca cgtgggcaaa caggcccccg gcaatccccg ccaggcccga
     1921 ggacagcatg aaggccacga tcttcatctt gcgggtgttg acgctcatga tctccgcggc
     1981 gatctcatca tcccgaaggg ccacgattcc tttcccgtag gtggatgtga cgaactgccg
     2041 gaggatccac aaggtgaatc ccacggaaat cacgatccag atcatgatcc agggcagatt
     2101 caccacctcc tgcatggaat tgacgatggc gcgcatgccc atgaatcccc gagccccgcc
     2161 gatgacctgc aggttctcga tggagctctt gacgatgtag ttcacggcca gggtgatgat
     2221 ggccaggtag tccccccggg ttcgaaagga gggaacggcc accaggagcc cggccagggc
     2281 ggcggcaacg ccccccagga gcagggtgat cgggaaaagg tacagggcgc tcgaaggcgc
     2341 aagcaccggc gggccgaaaa cctggctctg ggaaaacaac cacacgttca ggaccgaggt
     2401 ggtgtaggcg cccacggcca tgaatcccgc gtgacccacc gagaactccc ccatgtatcc
     2461 gttcaccagg ttcagagatg cggaaaggat gatgttgatc ccgatgtaca tgagaacgag
     2521 ctggagatag gcgttgatcc agttgccccc ggctgcggcg acgacgagca ggcccgggag
     2581 gatgaagcag aagacggaga tgcacttgcg catgtttggg ttcactcgct atatcttggc
     2641 ggctcgggcc accccgaaga tccccatggg cttgatgctc agaaaaacca gcaggatggt
     2701 gaaggaaatg aggtctcgaa gggtggaggg gaaaaaagcg gccacgaaga tttccacgaa
     2761 tcccagcagg aatcccccca cgaaggcccc tcgaatttcc ccgatacccc cgatgacggc
     2821 ggcgatgaag gctttccacc cgaccagggc ccccatgtaa ggctcgagaa cggggtaggc
     2881 cacggcgaac aggacccccg ccacggccgc catcgacgag cccaggatga aggtgaacac
     2941 aatgacggtg tccacgggga ttcccatgag ggggaccgcc atgcgatcgt aggagatggc
     3001 ccgcatggcc atgcccagct tggtcttgcg gaccaggaat tccagcagca gaaacaggag
     3061 aacggtggca atgacgacca ggaccttcac attgctgaac gtgatcccgg cgacggtgta
     3121 gacggtcttc tcgatgattt ccggaaagct gcgccggttg gcgcccagga gagcgagatt
     3181 tccgtgttcg agaatgaggc cgcacatgag ggcggtgatg accacgtaga gccgggaggc
     3241 tcccttgcgt cgaaggggcc gataggccac ccgctcgatg gccacaccca ctccggccgt
     3301 gaggatcatg gtgaaaagaa tcgtcagccc aaacaccatg gatggaggca gctggaaggg
     3361 aaagacggcc gagtggtggc ccaggagaat ccccgcgatg aaaaggccga ggtaggcccc
     3421 gaccatgaag atgtccccgt gggcgaagtt gatgagcagg aggaccccgt agaccatggt
     3481 gtagcccagg gcgatgaggg catagaaact gccccactgc agggcgttga ggaggttttg
     3541 cagaaaagtt tccatgaaag acggccgggc ctcccgttgg aatcacaaac atgcagaggc
     3601 gggaattcct tcccgcctct gcccggaaac caagcaagca ttgcccgccc cgctagggac
     3661 aggcttgttt gaagaattcg aattcgccct tgtcgctgat cttcacgacg acggcgcact
     3721 tggttgggtc cccgtgggct gagaaggtca tcctcccggt gatgccctcg aagtccttga
     3781 tcttgaccag ggcaccctgg atggcgtttc ggtccttgtc caggtccccg gtgaaaccgc
     3841 cggtgtcttt catggcctgg accaacaggc ccagggcgtc ccaggtgagg gccgccacgt
     3901 cgtcgggaac gtagccgtac ttggcgtggt acttgtcgat gaaatccttg gtggctccct
     3961 tggccccggc cgccgcgtaa tgggtgctga aatggtagcc cttgcaggag tccccgcaca
     4021 gcttcatgag ttccgcggac ccccagctgt cgctgcccat gatgggcttg ttccatccca
     4081 gttcccgggc ctgtttcacg atgaggggga cttcatcgta gtattgcgga gaaaacagga
     4141 cgtcggcgcc ggacttgacg atcttggtga gctgggcgct gaagtctttg tccttggtgg
     4201 taaacgtttc gaacgccacc accgatccgg ggccgtgaat cttttcgaag gcttccttga
     4261 agtattccgc caatcccttg ggataatcgc tggcaatgtc gtacaggacg gccgccttct
     4321 tggctttaaa ctcaagtttc gcacatgttt taagtgcttt ccaacatagc acgatcaatt
     4381 aa
//
LOCUS       DAOOVC010000243         4247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_11420_length_4247_cov_3.535067, whole genome shotgun sequence.
ACCESSION   DAOOVC010000243 DAOOVC010000000
VERSION     DAOOVC010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11420_length_4247_cov_3.535067"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..329
                     /locus_tag="PLM79_17675"
     CDS             102..329
                     /locus_tag="PLM79_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88189.1"
                     /translation="MAYTPELDLPTVRILRRIAWAMNEPMTVALPSIIQWVATRLDRQ
                     KICSSCRAPADCKECPFNTQDTSNTPPVKEK"
     gene            329..835
                     /locus_tag="PLM79_17680"
     CDS             329..835
                     /locus_tag="PLM79_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88190.1"
                     /translation="MRTFMGYQRTTCSCELCRVNCRFIPGYLLPEDLASMASFLGYSE
                     LSRFAEENLLASVGALVAKAGKLFRIRTIVPARDENGCCRFFDGKLCKIHSVAPFGCA
                     FFDSHQDPLQSQKLSALGLISISEQWRNKSHSIYCRIWHHLYCLGYRAPSPEESRKRM
                     YMFCAPPD"
     gene            1229..2338
                     /locus_tag="PLM79_17685"
     CDS             1229..2338
                     /locus_tag="PLM79_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88191.1"
                     /translation="MIQLKPEIEKIFHVLDMPLPTAQDLFSMQQDLARGVSLQSENGD
                     TVGIVADESTAKAAIGLTEFEAETAFALSLVKKGKFSQRVITEVKAQMIRKSGLMEFW
                     EPADIKEVGGLANLKAYITNRAKAFLPGNENLPKPKGILLVGIPGTGKSLSCKATASI
                     LGWPLIRLDIGGLKNSLIGESERRMREATRVIEAFGEGVIWLDEVEKAFAGAQSSGQT
                     DAGTTASMFGHFLTWMQETKAPILVMATANNISQLPPEFLRAGRFDSIFFVDLPTRSE
                     RMEILEIMNGKYGCEIPALVAADATAGFTGAEIEQLAKDTLFDGYDAAVQTVVPLSRC
                     MREEISSLRDWAKTRARMANSPEPDAKEIRKIRTA"
     gene            2391..2711
                     /locus_tag="PLM79_17690"
     CDS             2391..2711
                     /locus_tag="PLM79_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88192.1"
                     /translation="MDDDSKIIKFDPDRAPLRIRSAKFCRHKHIIVCEKSRTLECDAC
                     GASIDPFDFMLRWAKEDRFLEFRMKQLKDELKRLGEKLRKLKGMEKNARARLRRLGVK
                     HHED"
     gene            2757..3089
                     /locus_tag="PLM79_17695"
     CDS             2757..3089
                     /locus_tag="PLM79_17695"
                     /inference="COORDINATES: protein motif:HMM:NF041591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VVA0879 family protein"
                     /protein_id="HOV88193.1"
                     /translation="MPAEPIRMTKDEWLKKGRELFGDDMMQWKFVCPSCGHVQRPEDF
                     RPYKDRGATPNSALIECIGRYDGHMHVHIGSGESPCNYASYGLFNFNPVTVTDEDGTE
                     HRCFAFKE"
     gene            3094..3462
                     /locus_tag="PLM79_17700"
     CDS             3094..3462
                     /locus_tag="PLM79_17700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88194.1"
                     /translation="MTVKATRKPPAPLIIGPGWYQMQWPWENQRCWQHRTGLRVIQEI
                     EIHDGKEWLHTSFSRPDRIPSYQEITVVKDQFIGTAKKAIMVFPPREEHVNVHPFCLH
                     LYSCLAEDGLPDFRRAGGGL"
     gene            3694..3924
                     /locus_tag="PLM79_17705"
     CDS             3694..3924
                     /locus_tag="PLM79_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88195.1"
                     /translation="MFNDLAGGPISDGIDGHHLVLDWFKTVRGFRARCSCGTQFGPAG
                     GKGIDRAAFAYDRMAIKKKFNDHIARCSKANL"
BASE COUNT         1054 a         1223 c         1127 g          843 t
ORIGIN      
        1 ctactctcac ctttgcaact acctggatgc acgcccgaag gaaggtcagc tcacctttca
       61 ggacatcctc atgagagagc aatcacgcaa aggacttcgc catggcctac actccggagc
      121 ttgatctccc cacagttaga atccttcgca ggattgcatg ggccatgaac gagcccatga
      181 ctgttgcctt gccgtcgatc atccagtggg ttgccacccg cctggaccgg cagaaaatct
      241 gcagctcatg ccgcgccccg gcagactgca aggaatgtcc gttcaataca caagacacct
      301 ccaacacccc tccagtcaag gagaagtaat gcgaacattt atgggatatc agagaaccac
      361 ctgttcctgt gagctctgcc gtgtcaactg ccgcttcatt ccaggctatc tcttgcctga
      421 ggatctggct tccatggcat cttttcttgg ctactccgag ttgtcacgct ttgccgagga
      481 aaacctcctt gcatccgtcg gcgcactcgt agccaaggcc ggcaagctat tcagaatcag
      541 aaccattgtt ccggccaggg atgagaacgg gtgttgccgg ttcttcgatg gaaagctctg
      601 caagatccac tctgtcgctc cttttggatg cgccttcttc gacagccacc aagatcccct
      661 ccagagtcaa aagctctcag cactcggcct catctccatt tcagagcagt ggagaaataa
      721 aagtcattcg atctattgcc ggatttggca ccatctctac tgcctgggct acagagctcc
      781 atccccggaa gagagcagaa agagaatgta catgttttgt gcaccgccag attgaattgc
      841 agcctgtctt aggtgaattc cacttttcga atccaaccac aacaaagagg aggacacgat
      901 gatccgggac tacttgaaag ccgggtaccc ggcaattctt ttgctcacac aggagccgca
      961 ccgcgcggag aacatccttc cccatgaggg gtggtcgttt ctctcgtggg actgcctgcg
     1021 cggattccgc cagccgggaa agcctcaagc cattgacgag atccgtgacc cgatagaagc
     1081 aatcaaccgg ctcggggacg tgcaggacac cgttttgctc gcgcataacc tgcacctttt
     1141 cctcgacata ccggaagtga tccaggccct tcaaaacggc atcaccaggt ggaaatcaac
     1201 cggctgctgc ctggtcatga tctcccccat gatccagctc aagcccgaga tcgagaaaat
     1261 ctttcacgtt ctcgacatgc cgctcccgac cgcacaggat ctcttttcca tgcagcagga
     1321 cctggccagg ggagtcagcc tgcaaagcga gaacggggat acagtaggga tcgtcgcaga
     1381 tgagagcacc gctaaggccg cgatcggcct caccgagttc gaggcggaga cagcctttgc
     1441 tctttccctg gtgaagaaag gtaagttcag ccagcgcgtc atcacggaag tcaaggcaca
     1501 gatgatccga aaatccgggc tcatggaatt ctgggagcct gcggacatca aggaggtcgg
     1561 aggcctcgcg aacctcaagg catacatcac caaccgcgcc aaggcgtttc ttcctggaaa
     1621 cgaaaacctt ccgaaaccca aaggtattct ccttgtgggg attccaggca ccggaaagag
     1681 cctctcctgc aaggcgactg cctccatcct gggctggcct ctcatccgcc tggacatcgg
     1741 agggctcaag aactctctta tcggcgagag cgagcggcgc atgagggagg ccactcgagt
     1801 gattgaggcc ttcggagaag gggtcatctg gcttgatgaa gtggaaaaag cgttcgctgg
     1861 agcccagtcg agcggacaga cggatgccgg cacaactgct tccatgttcg gccattttct
     1921 gacctggatg caagagacca aggctcctat cctagtcatg gcaactgcaa acaacatctc
     1981 tcagcttcct cccgagttcc tccgcgccgg ccgcttcgat tccattttct ttgtggacct
     2041 tccgacccga agcgagcgca tggaaatcct tgagatcatg aatggcaaat acgggtgcga
     2101 gataccagct cttgtggcgg ccgatgccac ggcaggcttc accggggctg agatcgaaca
     2161 gctcgcaaag gacactctct tcgacggtta tgatgcggcc gttcaaaccg tagttccgct
     2221 ctcccgctgc atgagagagg agatctcatc cctcagagac tgggcaaaga cacgggccag
     2281 gatggccaac agccctgagc ctgacgccaa agagatcaga aagatcagga cggcgtaatc
     2341 gctttaagac gggcacatgg cggtagacca caagacggcg gagccgatgc atggatgatg
     2401 attccaagat catcaagttc gacccggacc gagccccgct ccgaattcgc tcagcgaagt
     2461 tctgccggca caagcatatc atcgtctgcg aaaagagccg gactctcgag tgcgatgcgt
     2521 gcggggccag cattgatcca tttgacttca tgctgaggtg ggcaaaggaa gacaggttcc
     2581 tcgaattccg catgaagcag ctaaaagacg agctcaagcg cctgggagaa aagctcagga
     2641 agctcaaagg catggaaaag aacgcaaggg caaggctcag gaggctcggt gtgaaacatc
     2701 acgaggactg atgcctgccc tcgcgccaaa accacagaca taaggaggga attcctatgc
     2761 cggcagaacc gattcggatg acgaaagatg aatggctcaa gaaaggcaga gagctttttg
     2821 gcgacgacat gatgcagtgg aagttcgtct gtccctcctg cggccatgtt caaaggccgg
     2881 aggacttcag gccctacaag gacaggggag ccaccccgaa ctcggccctg atcgagtgca
     2941 tcggcaggta cgacggtcat atgcacgtcc acatcggaag cggggaatcg ccctgcaact
     3001 acgcatcgta cggtcttttc aacttcaatc cggtcacggt cacagatgaa gacggcaccg
     3061 agcataggtg ctttgcattc aaggagtgat cctatgacag tcaaggcaac caggaaaccg
     3121 ccggccccgc tgatcatcgg tcccgggtgg taccagatgc aatggccatg ggagaaccag
     3181 cgctgctggc agcatcggac agggcttcga gtcatccagg aaatcgaaat ccatgacgga
     3241 aaggaatggc tccatacctc attctcacgt cctgaccgaa tcccaagcta ccaggaaatc
     3301 accgtagtca aagaccagtt tatcggaacc gcaaagaaag ccatcatggt cttccctcca
     3361 cgcgaggagc atgtaaacgt ccatcccttc tgcctgcacc tttactcatg ccttgcggaa
     3421 gacggccttc cagatttcag gagagcagga ggaggcttat gagccggcgc cttacatcga
     3481 aagctgttgc atctgccgca aggaattcca aggagccccg gaatacttcc tgccactcta
     3541 cgaaggcgaa gtagtcaatg ccgataagac cgatgactgg gccgccatgc cctgctgcga
     3601 acaatgctat ttcgatcatc agcacatgtg gggccatgat gatggccagt gagagaggat
     3661 ctccctgtga ccttaacgcc aaacgaaata cacatgttca acgatctcgc cggcgggccg
     3721 ataagtgatg gaatcgatgg tcaccacctg gtcctcgatt ggtttaaaac cgttcgaggt
     3781 ttcagagccc gctgttcttg cggcacacaa tttggaccgg caggaggcaa gggtatagac
     3841 cgagctgcct tcgcctatga tcgcatggcc atcaagaaga agtttaacga ccacatcgcc
     3901 cggtgctcaa aagcaaacct gtagccaatc ttgttattgg ttttccgtct gtcgagggtg
     3961 agcctcatgt ccctggggga agggggcggg ggcgccagcc accaccgaag tccgtctcat
     4021 cccaccgctt atccatgtag gaacaagaac cactttcaat gagctatcac gttctcttct
     4081 acgacgatgg aagaggactg caagtgtcgt ttaaagagag gaacactccc catgaagctc
     4141 acaccccaaa aactgctcaa atccatcgcc accacagact cacccgttgc cagaggattc
     4201 ggaaggctca tcatgtacta ccgcctcaat gaccgggagg aagacgc
//
LOCUS       DAOOVC010000244         4228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_11486_length_4228_cov_7.303858, whole genome shotgun sequence.
ACCESSION   DAOOVC010000244 DAOOVC010000000
VERSION     DAOOVC010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11486_length_4228_cov_7.303858"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLM79_17710"
     CDS             complement(<1..756)
                     /locus_tag="PLM79_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOV88196.1"
                     /translation="MKAYSHLKRKIRQRMEILEETLRELEELGALEGEAGRRWRTHLA
                     GVGRSIEDFVLRVAVVGSVKSGKSTLINALVGSDLLRRGAGIVTSFVTRIRTSDRLGG
                     WVEIKPWSQIIEELNDSARMLPDFEGDGNHEPVDIRRAEDRQGLRDRLRQVQNDRQQT
                     RGHVEPGFFALKGYLDGYDLLGSSVGEGVNRLLFDPTSIRQHQRFVGTQGQEVYLRDM
                     ELQVPIPWLGENVEIADCQGSDSPNPLHFALLQQ"
     gene            complement(1218..1613)
                     /locus_tag="PLM79_17715"
     CDS             complement(1218..1613)
                     /locus_tag="PLM79_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88197.1"
                     /translation="MPENTQDRSGRVKLLLVDDEAGYVQVLAKRMARRNIEVTPALSG
                     SEAIRVLRDGSFDVAVLDLKMEDMDGIEVLKIFKMMDPTMPVIMLTGHGSETAARQGI
                     EFGAYDYLTKPCDLMELIHKIREACGKKG"
     gene            complement(1628..2065)
                     /locus_tag="PLM79_17720"
     CDS             complement(1628..2065)
                     /locus_tag="PLM79_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOV88198.1"
                     /translation="MLKTVVMLVDDEVPFVDTMTRRLGKRNLSIVTAYSGYEALEKLR
                     EKDSNVDVVILDVKMPGMDGIETLRKIKQAHPLVEVIMLTGHATVETGIEGMKLGAFD
                     YLMKPCDIDQLMAKVKEAKNKKTQHEDKITQARIQEITLRRGA"
     gene            complement(2315..4075)
                     /locus_tag="PLM79_17725"
     CDS             complement(2315..4075)
                     /locus_tag="PLM79_17725"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88199.1"
                     /translation="MESRQYYRSLTRNMILTVILVSFAPLILTTLIIGYRFETSYREK
                     VLAHLVELVEKHQQNIDTFLNDKLSYVRVLASSFSLDQLKDEAFLRHKLAVLQGAYDG
                     VFVDLGLVNDQGVQIAYAGPLKLDHVDYSGSDWFKKAIEKPYTISDVFLGIRRQPHFI
                     VTARHKWAGHEFILRATIEFEAFNSLVESIRIGVTGSAFIVNSEGKFQTRPQSASVLD
                     GEVPASLLAHGRQDGSSPYGAVGQALRAVFDFSQGDLRRKDVSVGRFRHGGRDVIYVM
                     THLKGGEWILAYQQDETDAFSDLYQTRKLAILIGFLGGLAIVFMAFALSKRMVRYIEK
                     ADREKEMMNEQVIEAGKLASVGELAAGIAHEINNPVAIMVEEAGWIGDLLEEEEFRES
                     RNLEEFKTALQQIRTQGERCKEITHKLLSFARRTDPRTRPMQLNDLIEEVVGISEQPS
                     RYANVKIVKNLDPRLPEVCASPSEIQQVILNLINNAIDAIGPGGGTVQITSRQEGDKV
                     VLDVSDTGCGIPKSVLPRIFDPFFTTKPVGKGTGLGLSICYGIVHKMGGEITVNSAVD
                     LGTTFHVYLPLFCKGEEARS"
BASE COUNT          769 a         1324 c         1236 g          899 t
ORIGIN      
        1 ctgctgcagg agggcaaaat ggaggggatt gggagagtcg ctgccctggc agtcggcgat
       61 ctcgacgttt tcacccagcc agggaatggg gacctggagc tccatgtccc gaaggtagac
      121 ctcctggccc tgggtcccca caaaccgctg atgctgccgg atggacgtcg ggtcgaagag
      181 aagtcggttg accccctccc ccacggaact cccgaggaga tcgtagccgt ccaggtagcc
      241 cttgagggca aaaaagccgg gctcgacatg cccgcgggtc tgctgccggt cgttttgcac
      301 ctgccggagc ctgtcccgga ggccctgtcg gtcctcggcc ctgcggatat ccacgggctc
      361 gtggtttcca tctccttcaa agtccggaag catccgggcc gagtcgttga gttcctcgat
      421 gatctgggac cacggtttga tttcgaccca tccccccagc cgatccgagg ttcggatgcg
      481 ggtgacgaag gacgtgacga tccccgcacc tcttctcaac aggtcggacc cgaccagggc
      541 gttgatcagg gtgcttttcc cggatttcac ggaacccact acggccaccc tcaggacgaa
      601 gtcctcgatg gaacgaccca caccggccag gtgcgtcctc caccgccgcc cagcctcccc
      661 ttcgagggcg cccagctctt ccagttcccg gagggtttct tccaggattt ccatgcgttg
      721 tcgaattttg cgcttcaggt gggagtaggc tttcatggtc ctccgtcttg tcccgtgatg
      781 gatgagggtc cacagctttt cggaatcccc ggggtccttc ccggcgtttc aagaaccgcg
      841 caagtgcagt tatccagaaa atccgaaaaa aatcaaggaa agccctccac cgcttcctgc
      901 ggacagccca acattctgca cggatttctc gtttcgacga ggactgccgg accggctacc
      961 cggtccgggc catgggggga cggagtctcc cgggcagcgg cattcccgaa cggaggttgg
     1021 aagaggggtg ggtcggcccg gaaggaacct gggtcgggcc gctttttttc agtgccggtt
     1081 tgatggagag ggaaaacgtc cggatttcag gtccccgggc ggagattggg aacgagggaa
     1141 tcgctcgacc cggggggaag caatggaaga gtcttcacca cggggcaccc ggatgggcgg
     1201 gatcaaggcc tggagggcta ccccttcttc ccgcacgcct ctcggatctt gtggatgagc
     1261 tccatcaaat cgcagggctt ggtgaggtag tcgtaggcgc cgaactcgat tccttgccgg
     1321 gccgccgttt cggaaccatg tcccgtgagc atgatcaccg gcatcgtcgg gtccatcatt
     1381 ttgaagatct tgaggacctc gatcccgtcc atgtcttcca tcttgaggtc caaaacggcc
     1441 acgtcaaagc tgccgtctcg aaggactcga atcgcctcgg aaccgctcag ggctggcgtg
     1501 acttcgatgt tgcgccgggc catccgcttg gcgagcacct gcacataccc cgcctcgtca
     1561 tccaccagga ggagcttcac gcgaccggac cgatcttgtg tattctcagg catctccggg
     1621 ttcctgttca ggctccgcgc cgcaaggtga tttcctggat ccgcgcctgg gtgatcttgt
     1681 cttcgtgttg ggtcttcttg ttcttggctt ccttgacctt ggccatgagc tggtcgatgt
     1741 cgcaaggttt catcaggtag tcgaaggccc ccagcttcat cccttcgatt ccggtctcca
     1801 ccgtggcatg accggtcagc atgatcactt ccaccagggg atgggcctgc ttgatcttcc
     1861 ggagtgtctc gatgccgtcc atgccgggca tcttgacgtc caggatcacc acatccacgt
     1921 tgctgtcctt ttcccggagc ttttccaggg cttcgtagcc gctgtaggcg gtcacgatgc
     1981 tcaggttcct cttgcccagg cgtcgagtca tggtgtccac aaaggggact tcgtcatcga
     2041 ccaacatcac gacggttttc aacatgagga ttcctccctc tcttgacgtt acgacaggat
     2101 ttgactccaa agatacccca tttcccttgg tttcgaaaac ccccgaaacc cgcacccggc
     2161 cgtcggttgt cgactctcgg gggcggacgg tgactcggga ccgttgggcg gcccgcccca
     2221 tggaaaccgg gcggtgggaa ttttccgcgc cccctaagga aatccggcgc cgacgactcg
     2281 acccggccgg ccggcgatgg atctcgagat cggcctacga acgcgcctcc tcccccttgc
     2341 aaaagagggg caggtaaacg tgaaaggtgg tgcccaaatc cacggcactg ttcaccgtga
     2401 tctccccccc catcttgtgc acgatgccgt agcagatgga cagccccagc cccgtgcctt
     2461 tccccacggg cttggtggtg aagaacgggt cgaagatccg gggcagcacg gacttgggta
     2521 tgccgcatcc ggtatccgag acgtccagca ccaccttgtc gccctcctgt ctgctcgtga
     2581 tttggaccgt tcccccaccg ggcccgatgg catcgatggc gttgttgata aggttcaaga
     2641 tcacctgctg gatttccgac ggggaggcgc aaacctcggg caaccggggg tccaggttct
     2701 tcacgatctt gacgttggcg taccgggacg gttgctcgga aattcccacc acctcctcga
     2761 tgaggtcgtt gagctgcatg ggtcgcgtcc ggggatcggt tctccgggcg aaactcagca
     2821 gcttgtgggt gatttccttg cagcgctccc cctgggtgcg gatctgttgc aacgccgtct
     2881 tgaactcctc gagattgcgg ctctcccgaa actcttcctc ttccagcagg tctccgatcc
     2941 acccggcctc ttccaccatg atggcgacgg ggttgttgat ttcatgagcg atccccgcgg
     3001 caagttcccc caccgaagca agctttcccg cctcgatcac ctgctcgttc atcatctcct
     3061 tttcccggtc cgccttttcg atgtatcgca ccatcctctt ggaaagcgcg aaggccatga
     3121 agacgatggc cagccctccc aggaacccga tgagaatcgc cagcttccgg gtctggtaga
     3181 ggtccgaaaa ggcatcggtt tcgtcctgct ggtaggccag gatccattcc cctcccttga
     3241 ggtgcgtcat gacgtagatg acgtctctgc ctccatggcg gaaccgccca acggaaacgt
     3301 ccttgcggcg caggtcccct tgggagaagt cgaagacggc ccggagcgcc tgccccaccg
     3361 cgccgtaagg actcgagccg tcctgcctgc cgtgggccag gagactcgcg ggaacctccc
     3421 cgtccagaac cgacgcggac tggggcctcg tctgaaactt tccctcgctg ttgacgatga
     3481 acgcggatcc ggtcacgccg atccggatgc tttccaccag cgaattgaag gcctcgaact
     3541 cgatggtggc ccgcaaaatg aactcatgcc ccgcccattt gtgccgggcg gtaacgataa
     3601 aatggggctg ccttcgaatg ccgagaaaca catcgctgat ggtatacggc ttctcgatgg
     3661 cctttttgaa ccagtcggac ccggagtaat ccacatgatc gagcttgagg gggcctgcgt
     3721 aggcgatctg caccccttgg tcgttcacca ggcccagatc cacaaagacc ccgtcgtagg
     3781 cgccctggag aaccgccagc ttgtgcctga ggaaggcctc gtccttgagc tggtccaggc
     3841 tgaaggagct ggccaggacc ctcacgtagg agagcttgtc gttgagaaac gtgtcgatgt
     3901 tctgctggtg cttttccacc agctccacca ggtgggccaa aaccttctcc cggtaggacg
     3961 tttcgaaccg gtaaccgatg atcagggtgg tgaggataag gggggcaaag gagacgagga
     4021 tcaccgtgag gatcatgttc ctggtcaggg atcggtaata ctgcctgctc tccatgttct
     4081 gatccttcca gccccgaggc cgggagccct tcccttgggt cggacacaac ttcctaatac
     4141 gccgtcagca ggtacaccat gctcatggtg accgtgatca tcatgggcca ccagcacatc
     4201 ttcatgtatt cgacgaaaga gatcttgt
//
LOCUS       DAOOVC010000245         4219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_11517_length_4219_cov_6.729347, whole genome shotgun sequence.
ACCESSION   DAOOVC010000245 DAOOVC010000000
VERSION     DAOOVC010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11517_length_4219_cov_6.729347"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLM79_17730"
     CDS             <1..747
                     /locus_tag="PLM79_17730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOV88200.1"
                     /translation="GFGFLVLVLLESCAKPPPPSPTIPPPPGLRSQKPYQIRGIWYYP
                     LPTAEGYVEDGIASWYGKECHGNPTASGEPFDMNAMTAAHKTLPLGTHVKVTDLRTNR
                     SVILRVNDRGPFVAGRIIDLSRGAAQELGTARKGVARVRVEAVQVAREQYVGQNLYWR
                     VDPLPSFRYGSFAVQIGSFKDPDNAARLKTRMAKAYNQVEVSTFCNRGQNFFRVQVGA
                     FNDLVVAQQELQQLRGRGFPDAFVVATEEK"
     gene            749..2059
                     /gene="hisD"
                     /locus_tag="PLM79_17735"
     CDS             749..2059
                     /gene="hisD"
                     /locus_tag="PLM79_17735"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOV88201.1"
                     /translation="MLTITSYPSPDAEGLLQGIASRRPGVDPRLEQQVLEILHAVRKD
                     GDKALVRYTRTFDAPDLDASHLTVSREEIREACRAVDAGFLDILRRAVSNIEGFHREQ
                     LRSSYFMTKPDGTFLGQRISPVSAAGLYIPGGKGGETPLISSVLMNGVPARLAGVKDI
                     ALATPPRRDGSIDPHLLAAAEEVGVTRIHRMGSAWAVAALAYGTESVPRVDVVVGPGN
                     AYVTLAKKMVSGEVGIDLLAGPSEILIIADENANPDYLAADLLSQAEHDPMASGVLIT
                     PSRKVAEQTVQCLERQLNRLPRKTIARESLDRYGAVFLVEDLDTAASLAGRIAPEHLE
                     LQVRDPWSLVGKIEHAGAVFLGENTPEAVGDYFAGPNHVLPTAGTARFASALGVEAFL
                     KRTSVISYSRKALERDAEAVVRLAELEGLHAHAASVRIRLEDPR"
     gene            complement(2165..2488)
                     /gene="cutA"
                     /locus_tag="PLM79_17740"
     CDS             complement(2165..2488)
                     /gene="cutA"
                     /locus_tag="PLM79_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOV88202.1"
                     /translation="MSVVFVTVGNAEEGTRIAQTLVEERLIACANILPNVLSVYRWKG
                     EIYREEERLLILKTSSARFPALMKRIRELHSYEVPEIVSFPLTDGLPEYLQWVLDNSR
                     NEGDG"
     gene            2678..3331
                     /locus_tag="PLM79_17745"
     CDS             2678..3331
                     /locus_tag="PLM79_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88203.1"
                     /translation="MSFPVSGEYNRDQWNTIQGIRTILSQLPPEEIRRLEARIQPYLL
                     FREELEEFQARYFAKRCRDACFETRLSACCGFESIFTFFADHVVHFLVTGVEEGSRVL
                     APLERPNRTNRCVYLGPEGCLWKIRPLSCAMFLCESAKKEVFARHGETEEIWKGFLTR
                     EKEYTWPSRPVLFDEMEAYFIRRGVQSVHLYFHSSPGLLRVKAQAGLTPQVSGRMHS"
     gene            3434..>4219
                     /locus_tag="PLM79_17750"
     CDS             3434..>4219
                     /locus_tag="PLM79_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV88204.1"
                     /translation="MKYSPTILVVDDEPVTLQLIVEGLQEEGLNVDLASSGAEAIEKS
                     RQASYDIVITDLVMPGMDGMKVLDHFAGNFPDTSVILLTGYGTIETAVQAVKKGAYDY
                     LTKPAKLDEIVLLIQRAAELKALRTENVLLRSQLQDRYRYDRIIGQSAPMQALYRTIQ
                     RVAKADSTVLLMGESGTGKELIANAIHFNSDRRDKPLVPINCGAIPEELLESELFGHE
                     KGAFTGAIKERRGRFELAHQGTAFLDEIGEMSQKLQVKLLRFLQ"
BASE COUNT          832 a         1278 c         1317 g          792 t
ORIGIN      
        1 ggattcggtt tccttgttct cgttctcctc gaaagctgcg ccaaaccgcc tcccccttct
       61 cccaccatac ctcctcctcc gggcctgcgc agccagaagc cctaccagat ccgcggaatc
      121 tggtactacc ctctccccac ggccgaaggc tacgtggagg atggaattgc cagttggtac
      181 ggcaaggagt gccatgggaa ccccaccgcc agcggggaac ctttcgacat gaacgccatg
      241 acggcggccc ataagaccct gcccctgggg actcacgtga aagtgaccga cctgcggacg
      301 aaccggtccg tgatcctgcg ggtgaacgac cggggtccgt tcgtggcggg caggatcatc
      361 gacctgtccc ggggtgcggc ccaggagctg ggcacggctc ggaagggagt ggcccgggtg
      421 cgggtcgagg ccgtccaggt ggcccgggag caatacgtgg ggcagaacct ctactggcgg
      481 gtcgatccgt tgccgtcgtt ccgctacggg tcctttgccg tccagatcgg gtcgttcaag
      541 gacccggaca atgccgctcg tctgaaaacg cgaatggcca aggcctacaa tcaagtcgag
      601 gtgtccacct tctgcaaccg gggacagaat ttcttccgcg tgcaggtggg tgctttcaac
      661 gacctcgtag tggcccagca ggagttgcaa cagctccggg gccgggggtt cccggacgca
      721 ttcgtggtag caacggagga gaaatgacat gctgaccatc acttcctatc cctccccgga
      781 cgcggagggg ctcctccagg ggatcgcctc ccgaagaccg ggggtggatc cccggttgga
      841 acaacaggtg ctggaaatcc ttcacgcggt tcgcaaagac ggggacaagg ccctggttcg
      901 ctacacccgg accttcgacg caccggacct ggatgcatcg cacctgaccg tgagccggga
      961 agaaatccgg gaggcatgcc gggcggtgga tgcgggcttt ctggacattc tccggcgggc
     1021 cgtctccaat atcgaggggt tccaccggga gcagcttcga tcctcctact tcatgaccaa
     1081 gcccgacggg acctttctgg ggcagagaat ttcgccggtt tcggccgcgg gtctctacat
     1141 ccccggagga aaaggcgggg aaacccccct catttcctcg gtgctgatga acggggttcc
     1201 cgcacgcctg gcgggggtga aggacatcgc cctggcgacg cctcctcgga gggacggcag
     1261 catcgatcct catctgctgg cggctgccga agaggtgggg gtgacccgca ttcaccggat
     1321 gggcagtgcc tgggccgttg cggccctggc ctacggaacg gaatccgttc cccgggtgga
     1381 tgtggtggtg gggccgggaa acgcatacgt gacccttgcc aagaagatgg tgtccggcga
     1441 ggtggggatc gatctgctgg ccggtcccag cgagatcctg atcatcgccg atgaaaacgc
     1501 caacccggac tacctggccg ccgacctcct cagccaggcc gaacacgatc ccatggccag
     1561 cggagtcctc atcaccccga gcaggaaggt ggccgaacaa accgttcagt gcctcgaaag
     1621 gcagctgaac cggctgcccc gaaagaccat cgcccgtgaa tccctggatc gttatggagc
     1681 cgttttcctg gtggaagacc tcgatacggc ggcaagtctt gcgggaagaa tcgcaccgga
     1741 acacctggag cttcaggttc gggacccctg gtctttggtg ggaaaaatcg agcacgccgg
     1801 ggccgtcttc ctgggtgaaa acacccccga ggcggtgggg gactattttg cgggtcccaa
     1861 ccacgtcctt cccacggcgg gaacggcgcg ttttgcctcc gccctggggg tcgaggcttt
     1921 cttgaagcga accagcgtga tttcctattc gagaaaggcc ctggaacggg acgccgaagc
     1981 cgtcgtgcgc cttgcggagc tcgaggggct ccacgctcac gcggcgtcgg tgcgcatccg
     2041 gctggaagac cctcggtagc cggtcccctc gagaggtctt ccggtgggat ccccggcgaa
     2101 cggcttcgaa gccggaatag cccgagagcc gatacccctc ctcgaaagtc cccggtcgtt
     2161 cgggttatcc gtctccctcg ttccggctgt tgtccaggac ccactgcagg tattcgggca
     2221 ggccgtcggt caggggaaaa ctcacgatct cgggcacttc gtagctgtgc agctcccgga
     2281 tgcgtttcat aagggccggg aaccgggcgg acgaggtctt gaggatgaga agccgttcct
     2341 cctcccggta gatctcccct ttccaccggt agacggaaag aacgttgggg aggatgttgg
     2401 cacaggcgat gagcctttct tccacgaggg tctgggcgat ccgagttccc tcttcggcat
     2461 ttcccacggt aacgaagaca acggacacac cggtcaaggg gacctccttt tctctggggg
     2521 gacgcaactt gaatcttgag gatggaagtc tcccccgggc gcaggtccct gcccggccgg
     2581 aggagtcccc ggattcttgc aggaacctgc cgggtttgac aaccaagaaa aaacaggttc
     2641 gggtagtcct cccgggacct ggaaatacga ggagctcatg agctttcccg tttccggaga
     2701 atacaaccgg gaccagtgga acaccatcca gggcatacga acgatcctca gtcaattgcc
     2761 gccggaggaa atccggcggc tcgaggcccg gattcagccc tacctcctgt tccgggagga
     2821 attggaggag ttccaggccc gctatttcgc caaacgatgc cgggatgctt gcttcgaaac
     2881 ccggctcagc gcctgttgcg gcttcgagag cattttcacc ttctttgccg atcacgtggt
     2941 tcattttctc gtgacggggg tggaggaagg gtcccgggtg cttgcccccc tggagcgtcc
     3001 caatcggacg aaccgttgcg tctacctggg ccccgaaggc tgcctctgga agatccggcc
     3061 cctgtcctgc gccatgttcc tgtgtgagag tgccaagaag gaagtcttcg caaggcacgg
     3121 ggagacggaa gaaatctgga aggggttcct gacccgggaa aaagagtaca cctggccctc
     3181 ccgcccggtc ctctttgacg aaatggaggc gtacttcatt cgtcgggggg ttcaatcggt
     3241 ccatctctat ttccactcga gtcccgggct gcttcgggtt aaggcccaag cgggcttgac
     3301 cccacaagta tccgggcgaa tgcattcttg aattaatctt cgagtatgat agattttccg
     3361 ccatacatca tgggtgcttt cgggcgctcg ggcggggtga gattcattat ggaatttcga
     3421 agaacagggg tctatgaaat attcccccac tatcctggtg gttgatgacg agccggtgac
     3481 tctccaactg attgtggagg gcttgcagga agagggactc aacgtggatt tggcctccag
     3541 cggggccgag gccatcgaga agtctcgaca agcgagctac gatatcgtca tcacggacct
     3601 ggtcatgccc ggcatggacg gcatgaaggt cctggatcac tttgccggca acttccccga
     3661 caccagtgtc attctgctca cgggatacgg cactatcgag accgccgtcc aggcagtgaa
     3721 gaagggtgct tacgactatc tcaccaagcc cgccaagctg gacgaaatcg tcctgttgat
     3781 ccaacgggcc gcggagctca aagctctgag aacggaaaac gtgctcctgc gctcccagct
     3841 ccaggatcgg taccgctacg atcgcatcat cggccaaagc gcgcccatgc aggccctcta
     3901 ccgaaccatc cagcgggtgg ccaaggcgga ctcgacggtg ctcctgatgg gcgaaagcgg
     3961 cacgggcaag gaactcattg ccaacgccat tcatttcaac agcgatcgaa gagacaagcc
     4021 cctggtgccc atcaattgcg gggcgatccc cgaggaactc ctcgagagcg agctgttcgg
     4081 ccatgaaaag ggagccttca ccggcgccat caaggaaaga aggggccgct tcgaactggc
     4141 ccaccaggga acggcctttc tggacgaaat cggagaaatg agccagaagc tccaggtgaa
     4201 gctcctgaga tttctccag
//
LOCUS       DAOOVC010000246         4145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_11752_length_4145_cov_6.995355, whole genome shotgun sequence.
ACCESSION   DAOOVC010000246 DAOOVC010000000
VERSION     DAOOVC010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11752_length_4145_cov_6.995355"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /gene="selD"
                     /locus_tag="PLM79_17755"
     CDS             <1..559
                     /gene="selD"
                     /locus_tag="PLM79_17755"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOV88205.1"
                     /translation="NGGCRPGDALVLTKPLGTGVLATAMKAGLIPPHAERRAVEVMAA
                     LNRRAAEVMSSYPVHACTDVTGFGLLGHAREMAAASQMTLVLEVDRVPFLPESLESAQ
                     MGLVPAGSFSNRNSCSRQVLMDRAVDPVLLDLLADAQTSGGLLISVDGSRGRDLVEDL
                     HGAGCPDAALIGRVLEPGPGIIRLV"
     gene            complement(614..1609)
                     /locus_tag="PLM79_17760"
     CDS             complement(614..1609)
                     /locus_tag="PLM79_17760"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOV88206.1"
                     /translation="MVRSVVRGIGSYVPPKVVSNEDLSALMSTSDEWIRSRSGIRERR
                     FAEEGVYCTDLALEAVRRALGDADMEPSDLDFIIFATLSPDHHFPGNACYLQAKMGIS
                     DIGCLDVRNQCTGFLYSLAVADSFIRTGMYRNILVVGSEVHSSAMDFSDRGRDVAVLF
                     GDGAGAVILSPSENGDRGVLYTELHADGQYARALYMDVWDISRKPFLGPEAVQSDVIW
                     PKMDGKTVFKHAVIRLIQVFQSALKKSGVRPEDIKYVIPHQANMRINHLVAEKIGIPP
                     EKFLHNMQKYGNTTAASLPLLLDETYRAGKLEKGDLLLLLAFGSGFTWGASLLRW"
     gene            complement(1758..3293)
                     /locus_tag="PLM79_17765"
     CDS             complement(1758..3293)
                     /locus_tag="PLM79_17765"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HOV88207.1"
                     /translation="MDGDWLIAHMGAEPATLNPITATDASASTINNYLYESLLKRDEA
                     TLELIPQLAESWEISEDRLVYTFRLKRNIRWHDGHPFTARDVLFSFQRIRDPKADAAH
                     LRNYYQDIEELEAPDDYTVRYRYRVPYFRALEFCGGIPLLPAHRFAETDDFNRHPIGR
                     NPIGTGPFRFLHWDTGREIVLIRNEDYWGEKPHLDRVVFKVITNPTVTLQVLKKGGLD
                     YAGLRPIQWVKQTRSKRFEEQFQKISYYSPSYSYIGWNLRKPLFSDVRVRKAMTLLVD
                     RETLLKKILFGLGTVISGPFYLNSPDYDAGIRPYPYDPREAVALLRSAGWQDRDGDGI
                     LDREGLPFVFEFLISSGSKFAEQVATILQENLKQVGIKMEIRRLEWAVFIQRIQGRNF
                     DACTLGWSLSWESDPYQIWHSSQAEKGSNTVGFVNPEADRLMEEARREFDPVKRRRLY
                     HRFHAIVHEEQPYTFLFTTQALVAVSRRFQNVKAYPMGLAPRRWWVPRDLQRYGHAAV
                     SMP"
     gene            complement(3790..4026)
                     /locus_tag="PLM79_17770"
     CDS             complement(3790..4026)
                     /locus_tag="PLM79_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88208.1"
                     /translation="MRLSEMRETMEYLRDNAFALNRNEMPNSPEGAACPREQSSTHTH
                     NGEEEPASREREKKLELWMEDVKAFIRNIDVSKL"
BASE COUNT          790 a         1208 c         1292 g          855 t
ORIGIN      
        1 caacggggga tgtcgaccgg gggacgcatt ggtgctgacc aaaccgttgg ggacgggggt
       61 ccttgccacc gccatgaaag ccgggctgat tccccctcat gcggaacggc gggccgtgga
      121 ggtcatggcg gctttgaaca ggagggccgc cgaggtcatg agctcctatc ccgtccatgc
      181 ctgcacggat gtgacggggt tcgggctgct gggacatgcc cgggaaatgg cggcggcgag
      241 ccaaatgacc ctggtcctgg aagtcgaccg ggttcctttt ctgcccgaat ccctggaatc
      301 ggcgcagatg ggcctggttc ctgcgggaag cttttccaac cgcaattcgt gttcccgcca
      361 ggtcctcatg gaccgggctg tggatccggt cctgctggat ctccttgcgg acgcccagac
      421 gtccgggggc ttgctcattt ccgtggacgg gtcccggggg cgggacctgg tggaggacct
      481 gcacggggcg ggctgcccgg atgccgctct catcggccgg gttttggaac cgggtcccgg
      541 catcatccgg ttggtatagg ttgcaagaca ggctgatggg gcgttccggt ccccggaccc
      601 tcgaggatac aacctaccat cgcaggaggg aggctcccca ggtgaatccg gacccgaaag
      661 ccagcaagag cagcaggtcc cctttttcga gcttgcccgc gcggtaggtt tcgtccagca
      721 acagcgggag ggaagcggcc gtggtgttgc cgtatttctg catgttgtgg aggaacttct
      781 ccgggggaat cccgatcttt tcggccacca ggtgattgat gcgcatgttg gcctggtgcg
      841 ggatgacgta cttgatgtcc tcgggacgga ctccggattt tttcagggcg gattggaaaa
      901 cctggatcag gcggatcact gcatgcttga agacggtctt gccgtccatc ttgggccaga
      961 tgacgtcgct ttggacggcc tcggggccga ggaagggttt gcgggagatg tcccacacgt
     1021 ccatgtaaag ggccctggca tattggccgt cggcgtggag ctccgtgtag aggacccccc
     1081 ggtctccgtt ttccgacgga ctcaggatga ccgccccggc cccgtctcca aacaggacgg
     1141 ccacgtcccg tccgcgatcg gaaaagtcca tggcgctcga gtgcacctcg gaacccacca
     1201 ccaggatgtt tcgatacatg ccggtccgga tgaaggaatc ggccacggcc agggagtaca
     1261 gaaagccggt acactggttt cgaacatcga gacagccaat gtcgctgatt cccatcttgg
     1321 cctggaggta gcatgcgttg cccgggaagt ggtggtcggg actcagggtg gcaaatatga
     1381 tgaagtccag gtccgaaggc tccatgtcgg cgtccccgag ggcccgcctg acggcttcga
     1441 gggccaggtc ggtgcagtaa actccctcct cggcaaaacg gcgttcccgg atccccgacc
     1501 ggctgcggat ccactcgtcc gaggtgctca tcagcgcgga taggtcttcg ttgctcacca
     1561 ccttgggagg cacatagctt ccgatccctc gaacgacgct ccggaccata aatccctcct
     1621 tctccgtatt caacatgccc ataccgttcg caacgggtcc cctccggatt ttcgcggtgg
     1681 attctcggaa aatgattccc ttttgtcaat acggtttgat tcccggccgg gggcggcgaa
     1741 gcctcgatcc gggattctca gggcatcgaa accgccgcgt gaccgtatcg ctgcaggtcc
     1801 cggggcaccc accaccgacg gggggccagg cccatggggt aggccttcac gttttgaaac
     1861 cgcctgctca cggccaccaa tgcctgggtg gtgaaaagga acgtgtaggg ctgctcctcg
     1921 tggacgatgg cgtgaaaccg gtgatagagc cgccgacgct tgacgggatc aaattcccgg
     1981 cgggcttcct ccatgagccg atcggcttcg ggattcacaa accccacggt gttggaaccc
     2041 ttttccgcct gggacgagtg ccagatctgg taggggtccg attcccagct caggctccat
     2101 cccagggtgc aggcatcgaa attgcgcccc tgaatcctct ggatgaacac ggcccattcc
     2161 aggcggcgaa tctccatctt gattcccacc tgtttcaggt tttcctggag aatggtggca
     2221 acctgctcgg caaatttgga tcccgaagaa atcaggaact cgaagacaaa gggcaggccc
     2281 tcccggtcca ggatgccgtc tccatcccga tcctgccatc ctgcagacct cagcagggca
     2341 acggcctccc ggggatcata ggggtagggc cgaatccccg catcatagtc cgggctgttg
     2401 aggtagaagg ggcctgagat caccgtgccc agtccaaaca ggatcttctt cagaagggtc
     2461 tcccggtcca ccagcagggt catggccttg cgaacccgca cgtcggaaaa gaggggcttt
     2521 ctcaggttcc aaccgatgta ggagtagctg ggagagtagt agctgatctt ttggaactgt
     2581 tcttcgaatc gtttgctccg ggtttgcttc acccattgga tcggccgcag acctgcatag
     2641 tccagccccc ctttcttcaa gacctgcaag gtcaccgtgg gattggtgat gactttgaac
     2701 accacccggt ccaagtgggg tttctctccc cagtagtcct cgttgcggat gagcacgatc
     2761 tcccgtcccg tatcccaatg gagaaatcgg aacggtcccg tgccgatggg gttccggccg
     2821 atgggatgtc gattgaaatc gtcggtttcg gcgaatcggt gggcgggaag gaggggaatg
     2881 cccccgcaga attccagagc cctaaaataa ggaacccggt atcgatagcg gaccgtgtag
     2941 tcgtccgggg cctccagctc ctcgatgtcc tggtaatagt tcctgagatg agccgcatcc
     3001 gccttggggt ctcgaattcg ctggaaggaa aacagaacgt cccgggccgt gaacggatga
     3061 ccgtcatgcc accggatgtt tctcttgagt cgaaaggtgt agaccagccg gtcttcggag
     3121 atctcccagg attccgccag ttggggaatc aactcgaggg ttgcctcatc ccgcttgagg
     3181 agggactcat acaggtagtt gttgatggtg gatgccgagg cgtccgttgc ggtgatggga
     3241 ttgagcgtgg ccggctcggc tcccatgtgg gcgatgagcc agtcgccgtc cacggggacg
     3301 gcccgggttt ccgcggcaga aacaccaagg accggggtgc acaagccgca gagaaggatt
     3361 gggacgatca ggtggaaaaa tccgggacgg atcatcgcgc gaaagaacca aacgattccc
     3421 agggggaatc ctttcaggag gtttcatgag attccagtgc cgcctgcgac ccagagaaga
     3481 ggccccagga caccgcagcg atccccgacg gagcgcatgg gaaagcttgc acggcccggg
     3541 tgggcgcagc cgcattgcgg ttcccgcgat gaaatcccgg gacgtcgttg cttctatcac
     3601 ggggaaggga gggcaacaag tcaaattcgc ccctgcccgg cgggaactcc tcccagcccc
     3661 ttcttgtcct ccccgacgga gagaccctgc ccggggtccg cttgttggga gaaacaaaaa
     3721 accccttcct tctcgaccga aaggggtcat ttgtcgctct tggatgtgaa aagaggttac
     3781 aacgcttccc tacagtttgc tcacatcgat attgcgaatg aaagccttga cgtcttccat
     3841 ccacagttcg agctttttct cgcgctcccg ggacgcgggt tcttcctcgc cgttgtgtgt
     3901 atgggtggaa gactgctctc gagggcatgc ggcgccttcg gggctgttgg gcatctcgtt
     3961 cctgttcagg gcaaaagcgt tgtctctcag gtattccatc gtctctctca tttcggatag
     4021 tctcatagca ccctcctagg tcacggttgt ctcggtgaaa gtagaataat caccacgatc
     4081 tgccgtgtaa agctgtgaaa cccttggctt taggcaatga ggcccctttg ggaaaagccc
     4141 cgacg
//
LOCUS       DAOOVC010000247         3990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12323_length_3990_cov_5.846506, whole genome shotgun sequence.
ACCESSION   DAOOVC010000247 DAOOVC010000000
VERSION     DAOOVC010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12323_length_3990_cov_5.846506"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /gene="uvrA"
                     /locus_tag="PLM79_17775"
     CDS             complement(<1..2115)
                     /gene="uvrA"
                     /locus_tag="PLM79_17775"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="The UvrABC repair system catalyzes the recognition
                     and processing of DNA lesions. UvrA is an ATPase and a
                     DNA-binding protein. A damage recognition complex composed
                     of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities.
                     When the presence of a lesion has been verified by uvrB,
                     the uvrA molecules dissociate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase UvrA"
                     /protein_id="HOV88209.1"
                     /translation="MDRIVIKGARQHNLKNVDLSLPRDRLIVITGVSGSGKSSLAFDT
                     LFAEGQRRYLEALSLHSRYAVPHLDAPEVDEIQGLSPAIAVDQRSIVRSPRSTVGTLT
                     EISDFLRLLYARLGTLHCPVCRLEVRALSIPEMVGEIFRAWPLGSRLLILAPLGLVKE
                     REVPGILRKLKREGFARVRIDGQVLDLDPLPRFPRSLVYRVEVVVDRLILDSSKLQRF
                     RDSMELAAKMGGQQVRVYRPDGEEKTFSETARCTSCGGTFPELTPGLFSTQHPAGACA
                     ACKGLGAVCGEQRPKGRAPRDDTSEGESGEDPMDLEDGPGIPCPQCRGSGLGLISRSV
                     KLGGLAIHQVSEMGLEDLDRWLSSLDLSSSRSVIAERPLKEIRARLRTLKELGMGYLK
                     LNRTARSLSGGEAQRVRLTQQLGTPLSGVLYVLDEPSVGVHPRDHERLMRILFRLRDE
                     GNTVVVVEHDRETILGADHVVDLGPGAGLLGGEILFSGPPQDLMRQPNSLTGQYLSGR
                     KTIPVPTRRRKPRKGFLRIVGATGHNLKGITVEFPLGCLTCVTGVSGSGKSTLVFDTL
                     YAALARQLFASKNPGAPLERLEGWEKIQRVVLIDQLPIGRNPRSTPATYTGLFAHIRE
                     IFAQLPEARVRGYGPSRFSFNVKGGRCEGCKGEGRCSVDMVFLPDVSVTCPLCGGSRY
                     RDETLDVKFKGKSIAEVLRMTVH"
     gene            complement(2125..3192)
                     /gene="pdxA"
                     /locus_tag="PLM79_17780"
     CDS             complement(2125..3192)
                     /gene="pdxA"
                     /locus_tag="PLM79_17780"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOV88210.1"
                     /translation="MKPSSLGLKPRVAVTMGDPCGIGPEILVKVLASSDVRDICTPVV
                     VGDPGALRQAMALTGIRLDLRVIGEISVLDAPESPGQGIVLCPRELSGADIRYRNPSR
                     ATCEAVVLYIETAVRAAMTGAVRAVCTCPIHKAHLHRHGFPFPGHTEYLRDLTRAEHA
                     VMMLAGPKLRVSLVTVHQAIAEVPRVLTRERIAEVIRVTGEALVRDFGIESPRIAVAG
                     LNPHAGEEGAFGREESELIQPALEDARSPHWEVFGPYPSDTVFLRGHRGEFDAVVAMY
                     HDQGLIPIKLVHFHDAVNVTLGLPIIRTSVDHGTAYDLAGRGIADEGSLRSAIELAVF
                     MARNRLSSPVSRAGTAGPFSG"
     gene            complement(3300..>3990)
                     /locus_tag="PLM79_17785"
     CDS             complement(3300..>3990)
                     /locus_tag="PLM79_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOV88211.1"
                     /translation="KKVLLPRPDAGCPMADMITPEDLVAVRNRHPGVPLVTYVNSTAA
                     VKAESTVCCTSANSIQVVESFPEADTVYMSPDKNLAMYTARHTRKNVLAWKGYCPVHQ
                     GLSARRVEERKAQYPQAVFLAHPECRPEVIDLADKVVSTSGMLRCVRENPGRSYIIGT
                     ETGILYPMRKQNPNTEIHAASDNMVCPNMKKTAPEDVLRALETLEPRIVVPEEIRIRA
                     LKAVERMLATG"
BASE COUNT          761 a         1236 c         1246 g          747 t
ORIGIN      
        1 atgcaccgtc atccggagca cttccgcaat ggattttccc ttgaacttca catccagggt
       61 ttcgtcccgg taccggcttc ccccgcatag ggggcaggtg acggagacat cgggcagaaa
      121 aaccatgtcc acgctgcatc tcccctcccc cttgcatcct tcgcagcgcc ctcccttgac
      181 gttgaaggaa aagcggctgg ggccgtagcc cctcacccgg gcctcgggaa gttgcgcgaa
      241 gatctcgcgg atgtgggcaa acaaacccgt gtaagtggcg ggagtggaac gaggattcct
      301 tccgatgggg agctggtcga tgagaacgac ccgttgaatc ttctcccacc cttcgagtct
      361 ttccagcggg gcccccgggt tcttgctcgc aaacagctgc cttgccaggg cggcatacag
      421 ggtatcgaag acgagggtgc tcttgccgga cccggagacc ccagtgacgc aggtgagaca
      481 gcccagggga aactccacgg taataccctt tagattgtga cccgtcgccc cgacgatcct
      541 cagaaaaccc ttgcggggct tccggcgacg ggtgggaacg gggatggtct ttcttccgga
      601 aaggtattga cccgtcaggg aattaggctg cctcatcagg tcttggggag gtcccgaaaa
      661 gagtatctct cccccgagaa gtcccgcccc ggggcctagg tcgaccacgt gatccgcccc
      721 gaggatcgtt tcccgatcgt gttccacgac aaccaccgtg tttccttcgt cccgcagccg
      781 aaagagaatg cgcatgagac gttcatggtc ccggggatgg accccgacgc ttggttcgtc
      841 caatacatac aggactccgg acaggggggt tcccagctgt tgggtgaggc gaacccgttg
      901 ggcttccccc ccggaaaggc tccgggcggt tcggttcagc ttcagataac ccatgcccag
      961 ttccttcagg gtccggaggc gggcacgaat ttccttgagg ggacgctcgg cgatgacgga
     1021 ccgggaggaa gaaagatcga ggctcgaaag ccaccggtcg aggtcttcca gtcccatttc
     1081 cgaaacctgg tggatggcga ggccccccag ttttaccgag cgagagatca acccaagacc
     1141 gcttccccgg cattggggac acgggatgcc gggaccatcc tcgaggtcca tgggatcttc
     1201 ccccgactcg ccctccgagg tgtcgtcccg gggcgcccgg cccttgggcc tctgctcccc
     1261 gcacaccgcc cccagccctt tgcaggcggc acaggcacct gccggatgtt gggtggaaaa
     1321 caaaccgggg gtcaattccg gaaacgtacc gccgcaggag gtgcatcgag ccgtctcact
     1381 gaacgtcttc tcctccccat ccggacggta caccctgact tgctgtccgc ccatcttggc
     1441 cgccagttcc atggaatccc gaaaccgctg caacttcgac gaatccagga tgagtcgatc
     1501 gaccaccacc tccacccgat aaacgagact gcgcggaaaa cggggaagcg gatcgagatc
     1561 caggacttgt ccgtcgattc gcaccctggc aaaaccctcg cgtttcaact ttctaaggat
     1621 gccggggacc tccctttcct ttacgaggcc caatggggcc aagatcagca ggcgacttcc
     1681 caggggccat gcacggaaga tttccccgac catctccggg atggagaggg cgcggacttc
     1741 cagccgacaa acggggcagt gaagagtccc cagccgcgca tacagcaggc gaaggaaatc
     1801 cgagatctcg gtaagggtgc ccacggtgga tcggggtgat cgcacaatac tgcgctggtc
     1861 gacggcgatg gccgggctga gcccctggat ttcatcgact tccggtgcgt ccaggtgggg
     1921 gacggcgtag cgactgtgga gactgagggc ttcgaggtat cgtctttgtc cctcggcaaa
     1981 aagggtgtcg aaggccaggg acgacttgcc ggacccgctc acgcccgtga tcacgatgag
     2041 acgatcccgg ggaagggaga ggtcgacgtt tttcaggttg tgctgccggg ctcccttgat
     2101 gacgatccta tccatggcct tccgttatcc cgagaacggt ccggcggttc cggctcggga
     2161 aacgggggag gacaaccggt tacgagccat gaaaaccgca agttcaatgg ccgaccggag
     2221 acttccctca tcggcgatcc cccttccggc aaggtcgtag gccgttccgt gatccaccga
     2281 agtccgaatg atgggaagac ccagggtgac gttgacggca tcgtgaaaat ggacgagctt
     2341 gatgggaatg agcccctggt cgtgatacat ggccacaacc gcgtcgaatt ctccccggtg
     2401 gcctcgaagg aagaccgtgt cggagggata gggcccgaac acttcccagt ggggcgatct
     2461 tgcgtcttcg agggccggtt ggatgagttc gctttcctcc cgtccaaacg ctccttcctc
     2521 tcccgcgtgg gggttcaatc ccgctaccgc aatccgggga gactcgattc caaaatcccg
     2581 gacgagggcc tcgcccgtta cgcgaatgac ttcggcgatg cgttcccggg tgaggacccg
     2641 gggtacctcg gcgatggcct ggtgcaccgt caccaaggac acacgcagct ttgggcctgc
     2701 cagcatcatg acggcgtgct cggcgcgggt cagatcccgg agatactccg tgtgccccgg
     2761 aaatggaaaa ccgtgccgat gtaaatgggc cttgtggatg ggacacgtgc aaacggcccg
     2821 aacggccccg gtcatggcgg cacgaacggc ggtctcgatg taaaggacca cggcctcgca
     2881 ggtggcccgg gaaggatttc gataacggat gtccgcgccg gagagttccc ggggacagag
     2941 gacgatcccc tgtccggggc tttccggagc atcgaggacg gagatttccc cgatcacccg
     3001 aagatccagg cgaattcccg tgagggccat ggcctgccga agggccccgg ggtcccccac
     3061 gacaacgggt gtgcaaatat cccggacgtc cgaagaggcc aataccttca ccaggatctc
     3121 cggcccgatt ccgcacggat ctcccatggt cacggcaacg cgcggtttga ggccgaggga
     3181 ggaaggcttc atggactcga tcccgtgatt ccgatatgag gcccccgtgc cgatgggggc
     3241 aaggttttcc tgtcccggtg gtgcccggcg agggtatcct ttgccggatc cggtcgcccc
     3301 taccccgtag ccagcatccg ctccaccgcc ttgagggccc ggatgcgaat ctcctcgggc
     3361 acgacgatcc tgggctccag ggtttccagg gctctcaaga catcctccgg ggctgtcttc
     3421 ttcatgttgg ggcagaccat gttgtcggat gcggcgtgaa tctccgtatt cgggttctgt
     3481 ttcctcatgg gatagaggat tccggtttcc gttccgataa tgtaggacct gcccgggttt
     3541 tctcgaacgc agcgcagcat ccccgaagta ctcaccacct tgtccgccag gtcgatgacc
     3601 tcggggcggc actcggggtg ggccagaaac acggcctggg gatactgggc cttgcgctcc
     3661 tcgacccggc gggcactcaa gccttggtgg acgggacagt accccttcca ggcgaggacg
     3721 ttcttccggg tgtggcgagc agtatacatg gcgaggttct tatcgggcga catgtacacc
     3781 gtatccgcct ccggaaacga ctccaccacc tggatggaat tggccgaggt gcagcagacg
     3841 gtactttcag ctttgaccgc cgcggtggag ttcacgtagg tgaccagggg aacgccggga
     3901 tgccggtttc ttaccgccac gagatcctcg ggggtgatca tgtccgccat ggggcatccg
     3961 gcatcgggac gcggcaacag gaccttcttg
//
LOCUS       DAOOVC010000248         3977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12371_length_3977_cov_4.796277, whole genome shotgun sequence.
ACCESSION   DAOOVC010000248 DAOOVC010000000
VERSION     DAOOVC010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12371_length_3977_cov_4.796277"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLM79_17790"
     CDS             complement(<1..406)
                     /locus_tag="PLM79_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88212.1"
                     /translation="MQTIAESLRKQREQLMEDSSEGLLSRNTSLLEIAVISLYNRLAN
                     RLAQDTERFRSSGVVLAMGPFARGQIGPTMPVPVLLLEAESSPWQESWLDEITSPLSQ
                     AGWTVEATQGSVPQLLQWIEEDFGAFLRLLECR"
     gene            755..1381
                     /gene="hisH"
                     /locus_tag="PLM79_17795"
     CDS             755..1381
                     /gene="hisH"
                     /locus_tag="PLM79_17795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOV88213.1"
                     /translation="MIAILDYRAGNLTSVERALGHLGFECRITHSRSFIERAERIVFP
                     GVGAAGRSMESLRELGLDEVLRDRIRAGVPVLGICVGLQVLFDFSEENNTPCLGILPG
                     RVRLFPGDMQGDDGRGLKIPHMGWNGVRFLWDHPVFRDIPPESEFYFVHSYYPEPAVS
                     LHAAAETHYGISFVSAVSRDNLIAVQFHPEKSGPPGLKLLENFCRWNG"
     gene            1401..2180
                     /gene="hisF"
                     /locus_tag="PLM79_17800"
     CDS             1401..2180
                     /gene="hisF"
                     /locus_tag="PLM79_17800"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOV88214.1"
                     /translation="MLSKRIIPCLDVRDGKTTKGIRFRDNVDIGDPVEMARFYYEEGA
                     DEIVFYDITASSDRRPIMIDVVRRVAETIFIPFSVGGGIRTLDDMREVLLAGAEKVSV
                     NSAAVLNPDILCRGAESFGSQCVVLGMDVKRVAPSTRIPSGYEIVINGGRTATGMDAV
                     WWAREAQRRGAGEICLNSIDADGTQEGYELNLTRLISTSVGIPVIASGGAGRLDHLLD
                     VLTQGHADAALVASMVHFGTYTIRQIKEFLMGRGVKVRRTW"
     gene            2225..2569
                     /locus_tag="PLM79_17805"
     CDS             2225..2569
                     /locus_tag="PLM79_17805"
                     /inference="COORDINATES: protein motif:HMM:NF020400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HOV88215.1"
                     /translation="MKKVAIVGCGAYMESGYGCPGEWRCMKAAALGTGKFEGPSQLTA
                     FIACECPGRTTAPNLGMAIKLSEIRPDVIHLSSCMVNAKPACPYVSPVELASILEAKT
                     GIPVILGTHEYH"
     gene            complement(2795..3100)
                     /locus_tag="PLM79_17810"
     CDS             complement(2795..3100)
                     /locus_tag="PLM79_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOV88216.1"
                     /translation="MPEFGSALTHLTPDRKVSHSELVRAIRFMIAAEYEAVQLYMQLA
                     EATDNELAKEVLEDISREELVHAGEFLRLLKEIEPSEEAFYVEGAKEVEEIIEKMKK"
     gene            complement(3164..>3977)
                     /locus_tag="PLM79_17815"
     CDS             complement(3164..>3977)
                     /locus_tag="PLM79_17815"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOV88217.1"
                     /translation="GFSFYRRSVTDPGLLPQLKAEHPHLSYCFHLAAIVSVPYSLDHP
                     QETMDTNCTAVVEMLRQAEALGFRAFVFAGSAAEYGTDQRLPLLEEYADDDTVHLSPY
                     GLAKYLASRQVGRSPIGVSLRCFNIYGPLQDPSSPYSGVISRFIRMASRGEPITIFGD
                     GLQTRDFVYVSDVVEGYLRVACPDPSHPEIQPGVYNIGRGIGTSILDLAALIRELTRS
                     SSKLSFLEERAGDIRHSRADISALERATRWRPRVDLADGLARTIEWMQGTRD"
BASE COUNT          772 a         1213 c         1215 g          777 t
ORIGIN      
        1 accgacattc caggagcctc aggaacgccc cgaagtcctc ttcgatccat tgcagcaact
       61 gaggcaccga cccttgggtc gcctccaccg tccaccctgc ctgcgacagt ggagaagtga
      121 tctcatcgag ccagctttcc tgccagggag aggactccgc ttcgagaagc aggaccggca
      181 cgggcatggt gggtccaatt tgcccccgtg caaacggccc catggccagc accacgccgc
      241 tggaccggaa cctctccgta tcctgggcga ggcggttggc caggcggttg tacagggaga
      301 tgacggcaat ctccaggagg cttgtatttc ggctgagcag tccttcagag ctgtcttcca
      361 tgagctgctc tcgctgcttt ctcagcgact cggcgatcgt ttgcattggc tccatcctct
      421 atttcccgtt tgttgacgac ccctgatcga tccatcctgc gcggggttag aatattcgat
      481 aatgggcagt ttaggaagtt ctcatgcgtc ggggagagaa aagcgccgcg gaggagcctt
      541 ttcgaggacg accccggccc gggaaggccg agggatcgga accgaaaacc gcccccgagt
      601 ggatgcggtt gttaccggcc gtgccttccc ccagggggcg ttcgatcccc ggtcccgaaa
      661 atgccgtctc ttacttgacc ctccgcgaaa tcccggatat tcttccatct tcgccggtgc
      721 acaccccatt ggtttacagg aaaggagctc agaggtgatc gccatactcg attaccgggc
      781 cggaaatctc accagcgttg aaagggcctt ggggcatctg ggttttgaat gccggatcac
      841 ccattcccgt tcgtttattg agagggccga gcgaatcgta tttccggggg tgggtgccgc
      901 gggaagatct atggagagtc tccgggaact gggcctggat gaggtcctca gggatcggat
      961 tcgggccggg gtccccgtcc tgggcatctg cgtgggcctc caggtcctgt ttgacttcag
     1021 cgaggagaac aacacccctt gcctgggaat cctcccgggc agggtccgcc ttttccccgg
     1081 ggatatgcag ggagacgacg gcagggggct gaaaatcccc cacatggggt ggaacggggt
     1141 ccgtttccta tgggaccacc cggtcttcag ggatattcct cccgagagcg aattctactt
     1201 tgtccacagc tattaccccg aaccggcggt ttccctgcac gccgccgccg agactcacta
     1261 cgggatctcc ttcgtttcag ccgtatctcg ggacaacctg atcgccgtcc agttccaccc
     1321 cgagaagagc ggtcctccgg gcctgaagct gctggagaat ttctgcaggt ggaacggata
     1381 atgcactgga ggggacccgt atgctgagca aaagaatcat cccctgtctg gacgtccggg
     1441 acgggaaaac caccaaggga atccggttcc gggacaatgt ggacatcggg gacccggtgg
     1501 agatggctcg gttctattac gaagagggtg ccgacgagat cgtgttctac gacatcaccg
     1561 catccagcga ccgccgcccc atcatgatcg acgtcgtccg acgggtggcc gagaccatct
     1621 tcattccctt ttccgtcggc ggggggattc gtaccctgga cgacatgaga gaagtgctcc
     1681 tggcgggggc cgaaaaggta agcgtcaatt ccgcggccgt cctgaatccc gacatccttt
     1741 gccggggggc cgaatccttt ggaagccagt gcgtagtcct gggaatggat gtgaagcgcg
     1801 tcgctccttc cacccggatt ccctcgggat acgaaatcgt gatcaacggc ggcaggaccg
     1861 ccacggggat ggacgccgtc tggtgggccc gggaagctca acggcggggc gccggagaga
     1921 tttgcctcaa ttccatcgac gccgacggaa ctcaggaagg ctacgagctg aacctcaccc
     1981 ggctcatctc gaccagtgtt ggaattccgg tgatcgcctc agggggagcc gggcgcctcg
     2041 atcacctctt ggacgtactg acccagggac acgccgacgc cgctctcgtt gcctccatgg
     2101 tgcacttcgg gacctacacc attcggcaga tcaaggagtt cctcatgggg agaggagtga
     2161 aggtgcgcag gacctggtag ttttcatggt atctctgacg gcggacaaca gaaggaggag
     2221 ccgaatgaaa aaagtcgcca tcgttggatg tggagcctat atggagagcg ggtacggatg
     2281 tccgggggaa tggcgctgca tgaaggcggc cgccctggga acaggcaagt tcgagggacc
     2341 ctcccaacta acggccttca tcgcctgcga atgccccggc cgcaccacgg cgcccaacct
     2401 gggcatggcc atcaagctct cggagatccg gcccgacgta atccatttga gctcctgcat
     2461 ggtgaacgcc aaacccgcct gcccctacgt gagccccgtc gagctggcct ccatcctcga
     2521 agccaaaacg ggcattcccg tcatcctggg aacccacgag taccattaag ccggggggcg
     2581 gtcggccgga gaccgaagac aggcggccta cggtacgggg aagcccatcc gaagaccatc
     2641 ccctggggaa gtcacgaggg ctcggcgagc agccgggggc atccactttt ccacgagccc
     2701 cgggagtctt ccggccccac cccaccgtac tccgccatcc ccgcctgccg gcttcccgct
     2761 tcccggcttc ccgcttccca gcttcccgga aaagctattt cttcatcttc tcgatgatct
     2821 cctcgacttc cttggctcct tccacgtaaa aggcctcttc cgaaggctcg atctccttga
     2881 gaagtcgcag aaactctccc gcatgaacca gttcctcccg ggaaatgtct tccagcactt
     2941 ctttggccag ttcgttgtcc gtcgcctcgg ccagctgcat atagagctgc acggcttcgt
     3001 attcagcggc gatcatgaaa cgaatggcac gcaccagctc gctgtggctc accttgcgat
     3061 ccggagtcaa atgggtgagg gcacttccaa attccggcat gacacacctc cgtaaagatt
     3121 gggttgatca caatcaaagg ggacccggca gcccttcgca ttctcagtcc ctggtcccct
     3181 gcatccattc gatggttcgg gccaggccgt ctgcgaggtc cacccggggc cgccagcggg
     3241 tggccctttc cagggcggag atgtccgccc tcgagtggcg aatgtccccg gctcgctctt
     3301 ccaggaagct aagcttcgag gagctgcggg tgagttcccg aatcaaggcg gccaaatcga
     3361 ggatggacgt gccgattccc cgaccgatat tgtatacgcc gggctggatt tcggggtgcg
     3421 acgggtccgg gcacgctacc cgcagatatc cttcaaccac atcggagacg tatacgaaat
     3481 ctcgggtttg gagcccgtct ccaaagatgg tgatgggttc tccccgggag gccattcgga
     3541 tgaacctcga tatcacgccg ctgtaggggc tggacgggtc ctggagaggg ccgtaaatat
     3601 tgaagcagcg cagagagact ccgatggggc tgcggcccac ctgccgggac gcaagatact
     3661 tcgcaaggcc gtaagggctc aggtgaacgg tgtcgtcatc ggcatattct tcgagcagcg
     3721 ggagccgttg atcggtgccg tattccgcgg cggatcccgc gaaaacgaag gccctgaacc
     3781 ccagggcttc ggcctggcgg agcatctcga cgaccgccgt gcagttggtg tccatggttt
     3841 cttggggatg gtccagggag tagggcacgc tgacgatggc ggccagatgg aaacagtagc
     3901 tcaaatgggg atgttcggct ttaagctggg gcaagagtcc ggggtccgtc acggacctcc
     3961 ggtaaaagct gaacccg
//
LOCUS       DAOOVC010000249         3957 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12443_length_3957_cov_5.799590, whole genome shotgun sequence.
ACCESSION   DAOOVC010000249 DAOOVC010000000
VERSION     DAOOVC010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3957
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12443_length_3957_cov_5.799590"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..516
                     /locus_tag="PLM79_17820"
     CDS             280..516
                     /locus_tag="PLM79_17820"
                     /inference="COORDINATES: protein motif:HMM:NF020263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HOV88218.1"
                     /translation="MSDDPKERIIEWVKQQKKSKHYFNDLCKAVPEIKMMQAKKIVNE
                     LVNEGKLKYWSSGSTTMYMLPSEGDVEKEEKGMN"
     gene            708..>1105
                     /locus_tag="PLM79_17825"
     CDS             708..>1105
                     /locus_tag="PLM79_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HOV88219.1"
                     /translation="MALKHKTPLIDELEKGPWPSFVSDVKRMAAKGNAMCEDWLGIME
                     MSYEQKEGHWKHGGIVGVLGYGGGVIGRYCDRPDLFPGVAHFHTVRVNQPASKFYTTD
                     ILRKLCNICEARGSGLTNMHGSTGDIVFLGT"
     assembly_gap    1106..1205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1206..1838
                     /locus_tag="PLM79_17830"
     CDS             <1206..1838
                     /locus_tag="PLM79_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase, dissimilatory-type subunit
                     alpha"
                     /protein_id="HOV88220.1"
                     /translation="VAAIARADCSIIGTWRDKIRIDQEAVRAYVGGELVPHAGADGNR
                     DKFDIVKEVVDLCPTRCMDWDGKNLKIWDEDCTRCMHCIRVMPRALRPGTDTGATILV
                     GAKAPILEGAQLSSVVIPFLKMEPPYSEFHEFVEKMWDLWMENGKNRERLGEMIQRIG
                     MREFLKACDLTPDPRMVNTPRYNPYIFYDPAEVPGGWERDAKAFRERHQA"
     gene            1850..2950
                     /gene="dsrB"
                     /locus_tag="PLM79_17835"
     CDS             1850..2950
                     /gene="dsrB"
                     /locus_tag="PLM79_17835"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - hydrogensulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HOV88221.1"
                     /translation="MPFDPNNLMQDRITDIGPPKYDKFFPPVVKENYGKWKYHEILEP
                     GVLVHVAESGAELYSVRIGTARLISVDLIREICDIADKYCGGYLRWTTRNNVEFLVSD
                     KTKLEPLKQELKSRGNWFPVGGTGNSITNIVHTQGWAHCHTPAIDASGVVKSVMDDLF
                     EYFGSHKLPAQVRIALACCLNMCGAVHCSDIAILGVHRKPPFIEHDRVQNVCEIPLVI
                     AACPTAAIKPKKVGEYKSVEINNNRCMFCGNCYTMCPALPLADGEADGIALWVGGKVS
                     NVISTPMFSKLAVPFIPNEPPRWPTTVQTIRKIVVAYAEGARRYERVGDWINRIGWER
                     FFEKTGLEFRHEHIDDYRLAMTTWRTTTQFKF"
     gene            3363..>3957
                     /locus_tag="PLM79_17840"
     CDS             3363..>3957
                     /locus_tag="PLM79_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88222.1"
                     /translation="MGFDKPRLMIAALRGGSGKTLVTMGLAAAWNRLQGLRVVPFKKG
                     PDYIDAGWLSVAAGHPCYNLDPFLMEPADMLGSFLRRIHGADAALIEGNRGLFDGVDR
                     RGTYSSAELAKLLGTPVVLLVDGTKVTRTAAAMVLGCRQLDPEVPLAGVILNRVAGRR
                     HEQVLRGSIEDACGIPVLGVVPKDPGILFPERHMGLVP"
BASE COUNT          824 a         1096 c         1210 g          727 t
ORIGIN      
        1 ggggaggttt cccggggcgg ccgggaaccg ggaaacgcgc aggaccggaa tgccgggccg
       61 acggaacccg ggctgaggtc gggccgggta tggcccatgg aggggtttcc cggtgccggg
      121 acgttggttt gcggggggat tttttggttg acaaagggta gttgggcgta ttatggaggg
      181 aacgtgaatc aattcacgtt cacagtcccc tcttgtgtgt atcaggatat tcggtcctgt
      241 atcgagtaat gcatcgcaga ttcttcaagg aggcaagaca tgagtgacga tcccaaagag
      301 cgcatcattg aatgggtgaa acagcaaaag aaatccaagc actatttcaa tgatttgtgc
      361 aaagcggttc ccgaaatcaa gatgatgcag gccaagaaaa tcgtcaatga actggtgaac
      421 gaaggcaaac tgaagtactg gtccagcggc agcacgacca tgtacatgct tcccagcgaa
      481 ggggatgtgg aaaaagaaga aaagggcatg aactgagttc ccgcccaggt ccctgccaat
      541 gattcgatcc gaatcttccc aagaaacctg aacggaagca ccttgttttg gacccatgca
      601 gaccattttt ggaggaggtg aaggaggaga gacctagttc gttgtcgagg tgaagagaag
      661 ggtgaataca tttgatcaac cgagaacggc caatggaggt aaatggaatg gcgctaaaac
      721 acaagactcc tctcatcgat gagctcgaga aggggccgtg gcctagcttc gtaagcgacg
      781 tcaagaggat ggcggccaag ggaaacgcga tgtgtgaaga ctggctggga atcatggaaa
      841 tgtcctacga gcagaaggaa ggacattgga agcacggcgg gatcgtgggc gtgctcgggt
      901 acggcggagg ggtcatcggc cgttactgcg atcgtcccga cctgtttccc ggcgtggctc
      961 atttccacac cgtccgcgtg aatcagcccg cgagcaagtt ctacaccacg gacatcctgc
     1021 gcaagctttg caacatctgc gaagcccggg gcagcggact caccaacatg cacggttcca
     1081 ccggggacat cgtgttcctg gggacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnngtggc cgccatcgcc cgcgccgact gctcgatcat tggaacatgg cgagacaaga
     1261 tccgcatcga ccaagaagcc gtccgcgcct atgtgggcgg ggaactggtt ccccatgccg
     1321 gtgccgatgg gaacagggac aagttcgaca tcgtcaagga agtcgtcgac ctgtgcccca
     1381 cccggtgcat ggattgggac gggaagaacc tgaagatctg ggatgaagat tgtacgcgct
     1441 gcatgcactg catccgcgtc atgccgcggg ctctgcggcc cggcaccgac acgggcgcca
     1501 ccatcctcgt gggcgccaag gctcccatcc ttgaaggtgc gcagctctcc agcgtggtca
     1561 tccccttcct gaagatggag cctccatact ccgaattcca tgaattcgta gagaagatgt
     1621 gggatttgtg gatggaaaac ggcaagaacc gcgagcgcct cggggaaatg attcaacgca
     1681 tcggaatgcg cgagttcctc aaggcctgcg atctcacgcc cgatcccagg atggtcaata
     1741 cgccccgata caatccttac attttctacg atccggcgga ggtgcccggc ggctgggaac
     1801 gtgatgccaa ggccttccgc gaaagacatc aggcttagga ggtggagcta tgccgtttga
     1861 tcccaacaat ctcatgcaag accgcatcac ggatatcgga cccccgaagt acgacaagtt
     1921 tttcccgccg gtggtgaagg aaaactacgg gaagtggaag tatcacgaga tccttgaacc
     1981 cggggtcctg gtgcacgtgg ccgaatcggg cgcagagctc tactcggtga ggatcggaac
     2041 cgctcgactc atcagcgtgg atctcattcg ggaaatctgc gacatcgccg acaagtactg
     2101 cggcggttac ctgcgctgga ccactcggaa caacgtcgag ttcctggttt cggacaagac
     2161 gaagctcgag cccctcaagc aggagctcaa gagccgaggc aactggttcc ccgtgggtgg
     2221 aacgggcaac agcatcacca acatcgtcca cacccagggc tgggctcact gccacacccc
     2281 cgccatcgac gcctcgggcg tggtcaaatc cgtcatggac gacctgttcg agtatttcgg
     2341 ttcccacaag ctccccgcac aggtacgcat cgccctggcg tgctgcctga acatgtgtgg
     2401 tgccgttcac tgctcggaca tcgccatcct gggggtgcat cgcaaaccgc cctttatcga
     2461 gcatgaccgc gtacagaacg tgtgcgaaat cccgctggtc atcgccgcct gccccacggc
     2521 cgccatcaag cccaagaagg tcggcgaata caagagcgtg gaaatcaaca acaaccgctg
     2581 catgttctgc ggcaactgct acaccatgtg cccggctctg cctctggccg acggcgaagc
     2641 cgacggcatc gccctctggg tgggcggcaa ggtgtccaac gtaatctcga ctcccatgtt
     2701 ctcgaaactg gcggttccgt tcatccccaa tgagccgcct cgttggccca ccacggtgca
     2761 gaccattcgc aagatcgtag tggcctacgc cgagggagcc cgacgctacg agcgcgtggg
     2821 cgattggatc aaccggatcg gttgggagcg tttcttcgag aagaccggct tggagttccg
     2881 tcatgagcac atcgacgact accggctggc catgaccacg tggagaacga cgacccagtt
     2941 caagttctaa ggactggcct cgagaaatag ggaccccctt cacccctccc tctcctctcg
     3001 ggggagaggg aggggttccc cggctctgcg ggaaggtgcc ccaaacggct tcttcccagg
     3061 gcggcagcgg cggaaggctt ttcccactac cggatggaac gggttggaag ctctttgcgc
     3121 cgtgcttcga gagcttgagc ggcaagggtt tttcccctct catgaggggg agaggagatg
     3181 ggggaagggg gggaaggacc tttttgcgaa ggacctccca gttcgtacaa ccatgtatgc
     3241 tcccggcgga ctcgccgccc ctgcggggga ggagcgaccc cggggaatcc cgcccccccg
     3301 gggcggacaa cccttcatct cccgcatccg taccttcatg agtacttggt aagagtatcg
     3361 cgatgggttt tgacaagccg cgcctgatga tagccgccct gcggggtgga tcgggaaaga
     3421 ccctggtgac catggggctg gcggcggcgt ggaaccgcct ccaagggctt cgggtggtcc
     3481 ctttcaaaaa ggggccggac tatatcgatg ccgggtggct ctccgtggcc gccgggcatc
     3541 cctgttacaa cctggatccc ttcctcatgg agcccgcaga catgctgggt tctttcctga
     3601 ggcgcataca tggggccgat gcggccctca ttgagggcaa tcgcggcctg tttgacggag
     3661 tggaccgtcg agggacttat tcctcggcgg aattggccaa gcttctggga acgccggtgg
     3721 tgctcctggt ggacggcacc aaggtgacga ggaccgccgc cgccatggtt ttgggatgca
     3781 gacagctgga tcccgaggtg cccctggcgg gggtgatcct gaaccgggtg gcgggtaggc
     3841 ggcacgaaca ggtcctgcgc ggatccatcg aggacgcctg cgggattccc gtgttgggag
     3901 tcgtgcccaa ggaccctggg attctctttc ccgagcgcca catgggtctc gttcctc
//
LOCUS       DAOOVC010000250         3950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12463_length_3950_cov_4.425931, whole genome shotgun sequence.
ACCESSION   DAOOVC010000250 DAOOVC010000000
VERSION     DAOOVC010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12463_length_3950_cov_4.425931"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..623
                     /locus_tag="PLM79_17845"
     CDS             255..623
                     /locus_tag="PLM79_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88223.1"
                     /translation="MPYRESWENPELFCTLADGTKVYHTYPDDDANQGHLSYWFTLLP
                     SASESCADETDWAFDVRMLPSVSGNDVETDDGKREVIQKAYDAGLLLPYMNCPSGCGS
                     CAPLPYNNLRDSNSQPPHVQ"
     gene            899..1552
                     /locus_tag="PLM79_17850"
     CDS             899..1552
                     /locus_tag="PLM79_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOV88224.1"
                     /translation="MKLVAVVGSRKLPASWAGRVSQVVTDLFRRGCRIASGGALGADL
                     FALQAVVRLGREACKGSQVFLPGSVMQAPSVSREWLLQFEALGGSIIQGSVAGRATAR
                     SEYVGALFGRTVELVKASSGVVGFVSGPSKGTWFTCEQAARSGKQIVLFSADGPSSIH
                     SLGCGHWKPVGCWQGAWRWETSVPVGRRCRHGIIVECCSCGMNRAASYPKEAYDEAN"
     gene            1965..2408
                     /gene="rnhA"
                     /locus_tag="PLM79_17855"
     CDS             1965..2408
                     /gene="rnhA"
                     /locus_tag="PLM79_17855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOV88225.1"
                     /translation="MKIVNIYIDGACSGNPGPGGWGAVLLYQGHRKEISGSEPNSTNN
                     RMEMTAAIASLLMIKEPCIVHIHTDSKYVQEGITRYIHKWKANGWRNSNGQPVKNQDL
                     WEVLCEAAKMHSITWHWIQGHNGHHQNERADQLARMAIRKASRSN"
     gene            2667..2933
                     /locus_tag="PLM79_17860"
     CDS             2667..2933
                     /locus_tag="PLM79_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88226.1"
                     /translation="MRFITVGIVVLALFLGLQLPDMPERPAQAHKLTVKEMCRGPNHP
                     DWCTELYADLAHGREVREKLRKEGKKVEESQPLDEIEQSHMEGL"
     gene            2933..3271
                     /locus_tag="PLM79_17865"
     CDS             2933..3271
                     /locus_tag="PLM79_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88227.1"
                     /translation="MKKSISKAIVILAVFSLVLAASVAMAADTLVQAKVTDVKEKLDK
                     NGSPFSIIVIQEERTLNGVTYSADVICTAFRQAHEEAKILKPGDTVKMIAAKREYNGD
                     AAYTVRKILK"
     gene            3268..3828
                     /locus_tag="PLM79_17870"
     CDS             3268..3828
                     /locus_tag="PLM79_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88228.1"
                     /translation="MRLRRLWFIVKQRARVILSILAIYISVTGLVTFSLFITEESIQM
                     ATFGTWPAQEAKDWYLVREGCERIKDTNRALKLINNCLGWIQPLAFFAYRSYGKSTDS
                     YVAGLEAKIFANAPEIFEGRKITASFTPQEVRDTPDGYMHTNRRVTFISKERLPAGVS
                     CRITGLVSVEGNKVVIKEPDEITRQK"
BASE COUNT          890 a         1122 c         1093 g          845 t
ORIGIN      
        1 cggcatcgtc cccgctgaaa tccttcacga ttccatggcc caccatgccg tgtttcctca
       61 aatcgggaca gactgcacga cacaaagcac agtcgagggg ccgggagcga aagcgcctgc
      121 gaacctgcct ccatcttctc ttaccgattg gagcgaaact catcctctcg cgaactgttc
      181 gaacgatatc ccatatggga cccatgacgc ggatctcggc tatacccttg atcccgaaga
      241 ggaggaggtg caagatgccg taccgtgaat catgggaaaa tcccgagctc ttctgcacgt
      301 tggccgatgg aaccaaagtc tatcacactt atcctgacga cgatgccaac cagggccatc
      361 tttcttactg gtttaccttg ctgccttcgg catcggagag ctgcgccgat gagactgatt
      421 gggccttcga cgtgcgaatg ctcccttcgg tctccggcaa cgatgtcgaa actgatgacg
      481 gaaagcgaga ggtcatccag aaagcctacg atgccgggct tctgctgccg tacatgaatt
      541 gccccagtgg gtgcggatct tgcgctcctc ttccgtataa taaccttcga gacagcaatt
      601 cccagccacc acacgtgcaa tgacagcgat ttggatccgc gcatccttgg tgcaatgctg
      661 aatgtctggt aaccgatgcc acggaatcct ctccaatttg catccgctgt cgctcgtccc
      721 gggatgacca tctctcgatc cctctcccat tgaggagcct gaccgcagtc ctttgcggtt
      781 caggagacat cccgctgttt caatatcccc ggcgctcaag aattggattt ctgcctgccc
      841 cctgcaagcg tgaactggtc gtttttcgtg tctgtctctt tttctgggag gagtagccat
      901 gaaacttgtt gctgttgttg gttccaggaa gctgcccgca tcttgggcag gcagagtatc
      961 gcaggtcgtt acggatcttt tccgccgcgg ctgccggatc gcgtccgggg gagctttggg
     1021 agccgatctt ttcgcattgc aggccgttgt ccgcctgggt cgcgaagcgt gtaagggatc
     1081 tcaggtcttc cttcctggat cggtcatgca agcgccgtct gtctcgcgcg aatggctcct
     1141 ccagtttgaa gcactgggcg gaagcatcat tcaagggtcg gttgcgggaa gagctactgc
     1201 aaggtccgag tatgtcggcg cgcttttcgg ccgaaccgtt gagctcgtga aggcatcctc
     1261 cggagtcgtt ggtttcgtct ccggccccag caaaggcacc tggttcacat gcgagcaggc
     1321 ggcgagatcg ggaaagcaga tcgttctctt ttcggccgac ggtccctctt ccatccactc
     1381 actcggctgc ggacactgga agcccgtagg ctgctggcag ggggcatggc gctgggagac
     1441 ctcggttcca gtgggccggc gatgcaggca cggcatcatc gttgagtgct gcagctgcgg
     1501 aatgaacagg gcggcaagct accccaagga ggcatatgat gaagccaatt agatcgttcc
     1561 tgcagcacga actgaattcc atgcacctct tctgtaagtt acgccgcctg atgggaaagc
     1621 gaccggcaat ggcaatcgcg aaggcatggg agagctgcct cctctatcgt ttcatctacg
     1681 catgacccgg atggagggag cccatgggtg tcacggtcac agcctcgaca attccgggtg
     1741 gcgtcacccc catatgcgac aactgcggaa tagccctttg ctgggatttg tccgagtccg
     1801 agtacaggtc ggatgaggag ttctgggaca actggcgatg cagggactgc aatcctgact
     1861 atcgcggagc cctgaaacgt ttccggtccc ttccatctga gactcgacct cttcatcagg
     1921 gggattcccc atttccccag ccaccacaga gagaaaggca ggccatgaag atagtcaaca
     1981 tctacataga tggagcatgc tccggcaacc cgggccctgg gggctgggga gccgtccttc
     2041 tttatcaggg tcacagaaag gaaatctccg gttctgaacc caacagtacg aacaaccgga
     2101 tggaaatgac cgcggcgatt gcatcccttc tcatgatcaa agaaccctgc atcgtccaca
     2161 tccacaccga ctccaagtac gtgcaggaag ggatcactcg ctacattcat aagtggaagg
     2221 ccaacggctg gcggaacagc aacggacagc cggtcaagaa ccaggacctt tgggaggtcc
     2281 tctgtgaggc ggccaagatg cactccatca cttggcactg gatacaggga cacaatggcc
     2341 atcatcaaaa tgagcgggcc gatcagctgg caaggatggc gatccgcaag gccagtcgct
     2401 ctaattgatg ggatttctgt ctgcctttgg tgaattcggt ctgcaccttt tctccccagc
     2461 gacgcttatg tggagctcga cacataagcc atgcgagtgc ggctagaggt cagctgggta
     2521 gtgctccccg ccgccacggg caggaaattc cttcggtatc tcaaaaggtt cctgccaggg
     2581 cagcctgaga ggaagcgcgg ccaagattgt gttccatggt gtgatgtgaa tgtctgtcgc
     2641 aacacgcgac acaaaggagg tcaatcatgc gattcatcac tgttggcatc gtagtgcttg
     2701 ctctgttcct gggcctgcag ttgcccgata tgccggaaag accggcccag gctcacaaac
     2761 tcactgtcaa ggaaatgtgc cgaggtccga accatcccga ctggtgcact gagctctacg
     2821 cggacctggc tcacggaagg gaggtgagag agaagctcag aaaggaaggg aagaaggtcg
     2881 aggaaagtca accgttagac gaaatcgaac aatctcacat ggagggtctc taatgaagaa
     2941 atccatcagt aaggcaatcg tcatcctggc cgttttctca ctcgtcctgg cagcctccgt
     3001 tgccatggcc gcggacaccc tggttcaggc aaaagtcacc gatgtgaagg agaagctcga
     3061 caagaacggc agccccttca gcatcatcgt catacaggaa gagcggactc tgaacggagt
     3121 cacctactcc gcggatgtga tctgtaccgc attccgccag gctcacgagg aagcaaagat
     3181 cttgaagccc ggcgataccg tcaagatgat tgcagccaag cgcgagtaca atggtgatgc
     3241 cgcctatacc gtccgcaaga tcctcaaatg aggctccgaa ggctctggtt cattgtgaag
     3301 caacgcgcca gggtcattct ctcgatcctg gccatctaca tctcggtaac aggtcttgtg
     3361 acattctcgc ttttcatcac ggaagaatcg atccaaatgg ccaccttcgg aacctggccg
     3421 gcccaggaag ccaaggactg gtatcttgtc cgagaggggt gtgagcgaat caaggatacg
     3481 aatcgggcac taaagctcat caacaactgt ctcggttgga ttcagccgct tgccttcttt
     3541 gcctaccgct catacggcaa atcaaccgac agctacgttg cggggctgga agcaaagatc
     3601 tttgccaacg cccccgagat cttcgagggc aggaagatca ccgcctcgtt cactccccag
     3661 gaagtccgcg atacacccga tggctacatg cataccaatc gccgtgtcac tttcatttca
     3721 aaggagagac tgcctgccgg cgtttcctgc cggatcaccg ggttggtatc agttgaaggc
     3781 aacaaggttg tcatcaaaga gccggatgaa atcactcgac agaagtaaac aagcctgccc
     3841 tgaggtcaag gaatgtgcga ggcagcatgc ccattgctgt ctcgcgactc tgtactgcaa
     3901 cttcaaaatc cgtcccggtt gaaattggat tacagcctgc cttccgcgaa
//
LOCUS       DAOOVC010000251         3915 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12598_length_3915_cov_12.499482, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000251 DAOOVC010000000
VERSION     DAOOVC010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12598_length_3915_cov_12.499482"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..519)
                     /locus_tag="PLM79_17875"
     CDS             complement(226..519)
                     /locus_tag="PLM79_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88229.1"
                     /translation="MTRMEMILDARIEALSQKMRRIEDDVRMMRNIMKSDLDTKEQIK
                     RVLSEALDEHVRERKTSHVPVVGVKVIPLRGGLVRPPWNSELNPLQNPNEPAK"
     gene            complement(512..1222)
                     /locus_tag="PLM79_17880"
     CDS             complement(512..1222)
                     /locus_tag="PLM79_17880"
                     /inference="COORDINATES: protein motif:HMM:NF022018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HOV88230.1"
                     /translation="MKELVTIDGMQVQRIEYREKPVVTLRMVDRLHQRPTGTAKRVFG
                     VHRDHLTEGQDYYVVPFQEWSKLQQDAIRPSEKSKSHNHMIFLTQYGYLMLVKVFRDH
                     RAWKVQRELVNTYFAPREDRAVAVEDRFHEGLGMGIQLGRMLERHGMDPADLARFCYY
                     RHIGLTQKETARLFETTRDKVQAVERTLLELGVEIPPSIPSPERKIRMMQLLDRLLGV
                     RAEVPQLRIAAASEVSHD"
     gene            complement(1446..1946)
                     /locus_tag="PLM79_17885"
     CDS             complement(1446..1946)
                     /locus_tag="PLM79_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88231.1"
                     /translation="MRLVCPSCGAVHSLEAWRNEPAWRQFAEALVKLPGPVQSRTLPY
                     LALFRRGERGLTAVRALKILDELVGLVALGTIRWEGGEERPAPPQLWAEALDAVLERR
                     PQGLRNHNYLRHVAWETARGLAAKIERDREAARIGGRPDPDQDMPSDEERAQIAEMIR
                     QFTRKH"
     gene            complement(1959..2636)
                     /locus_tag="PLM79_17890"
     CDS             complement(1959..2636)
                     /locus_tag="PLM79_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88232.1"
                     /translation="MDSRSRIDKTLRFGRLDPDERLVLEEILRAGYGANGSPLGRAGA
                     LSAAELARKVFGPPMRPGDQDTMKRAVRRVVNSLIIRHKIPICCEPGFGGGYYLPADD
                     SEVEANHHRFHRRAMTGLMKATRAKSSAYADSLIQLSFGYDGPEGREIRERYEMPEPN
                     PEGPPLWVSVVTKFLDRLKDDPDRYARELRQLQDRYGELMVPRRRVRQLKSLAGALKN
                     VVERLEG"
     gene            complement(2602..3057)
                     /locus_tag="PLM79_17895"
     CDS             complement(2602..3057)
                     /locus_tag="PLM79_17895"
                     /inference="COORDINATES: protein motif:HMM:NF018011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HOV88233.1"
                     /translation="MARGTRLIGKREKALLHVARQQLGLSEEAYRDMLASVGCRSSVE
                     LDAGRFDEIMRRLEAGGFRRKRRHSSAPASGMDRSSTPEREPLLSRIGAELTNQKLPW
                     SYADAIAGQMYGIRFVRWCEPMQLRAVLTALIKRKRKRDGLTIQDRQDT"
     gene            complement(3061..3333)
                     /locus_tag="PLM79_17900"
     CDS             complement(3061..3333)
                     /locus_tag="PLM79_17900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88234.1"
                     /translation="MMIMHLSKHFRQRWRARVSLGDPPCLDEVNEILRRSVRIRNQIR
                     LWKMRGRKFVPHRVLAEYWYHPGGLILKIDETNRTAVTVITAAGRR"
     gene            complement(3343..3609)
                     /locus_tag="PLM79_17905"
     CDS             complement(3343..3609)
                     /locus_tag="PLM79_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88235.1"
                     /translation="MLVDRSTIILTDERLDYLADRFLSRRIREILGMTFREYLRDPGW
                     YDAILDHLEAGRALVVSDGGDTLAVVQFCGSCGRVVAEVPVSGN"
     gene            complement(3599..>3915)
                     /locus_tag="PLM79_17910"
     CDS             complement(3599..>3915)
                     /locus_tag="PLM79_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88236.1"
                     /translation="EPGPVDSQTDGGIMARALTQPPRHTGAVYRQARLEFPVVSIPRD
                     DLRSMWQIGRGFDGAIRLVRDRMAQEGLDYGMPFRSWFDEGGALCHQNMPPRRTEAPN
                     AG"
BASE COUNT          680 a         1304 c         1153 g          778 t
ORIGIN      
        1 cctttaaggg gggcgacggg gacgatgaat ccgccccgtc gtctggaggc tccgtttcgg
       61 ctatgcgggc caggatgccc gatgccgccc tgcgcttctt cgcgctttat gccccggaac
      121 ttgatgccgc tgaagcggac gctctccggg gaagttaccc tcgatggggg acgatgtctg
      181 tcggggggcg accccgttgg agacgcccaa cccgattaca cttttctatt tagcaggttc
      241 atttggattt tgcaaggggt tcaattcgga attccagggc ggcctcacca atccgccccg
      301 caacgggatc accttcaccc caaccacggg aacgtgggac gttttgcgtt cccggacatg
      361 ctcatccagg gcttcgctca ggacccgctt gatctgttcc ttggtgtcaa ggtcggactt
      421 catgatattc cgcatcatcc ggacatcgtc ctcgattctg cgcattttct gcgacagggc
      481 ctcgatcctc gcatccagga tcatctccat cctagtcatg ggacacctcg cttgcggcag
      541 caatacggag ctgcggcacc tcggctcgaa ccccaagaag ccggtccagg agctgcatca
      601 tgcggatctt ccgctcggga gacggaatcg agggcggaat ctccaccccg agctccagca
      661 gggtgcgctc gacggcctga accttgtcgc gggtcgtctc gaacaaccgg gccgtctcct
      721 tctgggtgag gccgatgtgc cggtagtagc agaaccgggc gagatccgcc gggtccatcc
      781 cgtgccgctc gagcatgcgc cccaactgga tgcccatgcc cagcccctcg tggaaccggt
      841 cctccacggc aaccgctcgg tcctcgcgtg gggcaaagta ggtattcacc agttcccgct
      901 ggaccttcca ggcgcgatga tccctgaaca ccttgacgag catgagatag ccgtactggg
      961 taaggaagat catgtgattg tggctcttgg atttttcaga gggacgtatt gcgtcctgtt
     1021 gaagtttgga ccactcttga aacggcacca cgtagtaatc ctgtccctcg gtcaaatgat
     1081 cccggtgaac accgaagact ctcttggccg tgcctgtcgg ccgctgatgg agccggtcca
     1141 ccatgcggag agtgaccacc ggtttctccc ggtactcgat tcgctgaact tgcatcccgt
     1201 cgatcgtgac cagttctttc atgacctttc tcctgtattc ggcgtcccgg agactccccg
     1261 ccgcgataaa aaaatcccgg agacctgccg cgcctacagg aagcgccccc gccctcgcgg
     1321 aatcggggaa tctccgggaa atcgattttc ggccaataaa aaaggcctct gacgggggct
     1381 gacccgcctg tagattcggc acccataatt ttgcccacgc ccgaggaaaa tgtcaaggga
     1441 aaacctcagt gcttccgggt gaactgccga atcatttcgg cgatctgtgc ccgctcctca
     1501 tcggacggca tgtcctggtc gggatccggc ctgccgccga tccgagccgc ctcgcggtcc
     1561 cgctcgatct ttgctgccag tccccgggcc gtctcccagg cgacgtgccg caggtagttg
     1621 tgatttcgaa gcccctgggg acggcgctcg agcacggcat cgagggcctc ggcccagagt
     1681 tggggggggg ctggccgttc ctcgcccccc tcccaccgga tggtgccgag ggccaccagg
     1741 ccgacgagct cgtccaggat tttgagggcc cggacggccg tgagcccccg ttctccccgg
     1801 cggaatagcg ccaggtaggg aagcgtcctc gattgcacgg gccccgggag tttcaccagg
     1861 gcctcggcaa actgcctcca ggcgggctcg tttcgccacg cctccaaact gtgcacggct
     1921 ccgcaggacg ggcacaccag gcgcatcagg ccggcctcct agccttcgag cctctcgacc
     1981 acgttcttga gggccccggc caggctcttg agttggcgca cccggcggcg cggcaccatc
     2041 agttccccgt agcggtcctg gagctggcgc aactcgcggg cgtaccggtc cgggtcgtcc
     2101 ttcagcctgt ccaaaaactt cgtgaccacc gacacccaca agggcggtcc ttccgggttc
     2161 ggctcgggca tctcgtatcg ctcgcggatt tcgcgtccct cgggtccgtc gtatccgaac
     2221 gacaactgga tgagcgaatc cgcgtaggcg gaactcttgg ccctcgtggc cttcatcagg
     2281 ccggtcatgg ctcgtcggtg gaaccggtga tgattggcct ccacctcgct gtcgtccgcg
     2341 ggcaggtagt agcccccgcc gaatcccggc tcacagcaga tcgggatctt gtggcggatg
     2401 atcaaggagt ttactacccg ccgcacggct cgtttcatgg tatcctggtc cccgggcctc
     2461 ataggaggcc cgaagacctt gcgggccagt tccgcagcgg agagggcccc ggcccgcccc
     2521 aggggacttc cgttcgcgcc atagccggcc cggaggatct cctcgaggac caggcgctca
     2581 tccgggtcga gccgtccgaa tctaagtgtc ttgtcgatcc tggatcgtga gtccatcgcg
     2641 tttccttttg cgtttgatca gcgccgtgag tacggcgcga agctgcatcg gttcgcacca
     2701 tcgcacgaac cggatcccgt acatttgccc ggctatcgcg tccgcatagc tccaggggag
     2761 cttctgattc gtcaattccg ccccgatcct cgagagcagc ggttcacgct cgggggtcga
     2821 agaccgatcc atcccggagg cgggcgcaga ggaatgccgg cgcttcctcc gaaatccgcc
     2881 ggcctcgagc cgacgcatga tctcgtcgaa ccgaccggca tccagttcca ccgacgaccg
     2941 gcagccgaca ctcgccagca tgtcacggta cgcctcctcg ctcaggccca attgctgccg
     3001 ggcaacgtgc agcaaggcct tttcgcgttt cccgatcaat cgagttcctc tcgccatttc
     3061 ctacctccga cccgcggccg taatgacggt cacggcagtc cgattggtct cgtcaatctt
     3121 gagtatgagt ccacccgggt ggtaccagta ttccgccagg acccgatggg gcacaaactt
     3181 cctgccgcgc attttccata atcgaatctg gttccggatg cggacactcc gacgcaggat
     3241 ctcgttgact tcatcgaggc acggcggatc gccgaggctg accctggccc gccagcgttg
     3301 gcgaaagtgt ttcgagaggt gcatgatcat catccgctcc tcctagttgc cggatacggg
     3361 gacctcggcg accacccgtc cgcaagaccc gcaaaactgc accacggcca gtgtatcgcc
     3421 cccgtcactc acgaccaggg cgcggccggc ttcgaggtga tcgaggatcg cgtcgtacca
     3481 acccgggtcc cggaggtact ctcggaatgt cattccaaga atctcgcgga tcctgcggga
     3541 caggaaccgg tccgccaggt aatcaagacg ctcgtccgtc aaaatgatcg tgctgcggtc
     3601 aaccagcatt gggggcctcc gttcgcctgg ggggcatgtt ttggtggcac aatgcgccac
     3661 cttcatcaaa ccagctccga aagggcatcc cgtaatcgag tccctcctgg gccatgcgat
     3721 cccggaccag tcggatcgcg ccgtcgaatc cccggccaat ctgccacatg gatcgcagat
     3781 cgtcccgggg gatcgacacg accgggaatt ccaggcgtgc ttgccgataa accgcgccgg
     3841 tatgccgcgg cggttgcgtc agtgctctcg ccatgatgcc tccatctgtt tgagaatcca
     3901 caggacctgg ttctc
//
LOCUS       DAOOVC010000252         3907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12634_length_3907_cov_6.122534, whole genome shotgun sequence.
ACCESSION   DAOOVC010000252 DAOOVC010000000
VERSION     DAOOVC010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12634_length_3907_cov_6.122534"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1467
                     /locus_tag="PLM79_17915"
     CDS             298..1467
                     /locus_tag="PLM79_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV88237.1"
                     /translation="MTQPHDGSNTTSGDKDPRVIFLPRDGDGLVVRLTGHWNLAEGLL
                     STDSARREIEGHPTLKTVSFEAWDLAGWDSSLLSYLVQLHQVCVQKNLDVEWDRLPEG
                     LRKLLVLATAVPESKEARRREGRSSLLRRIREGLSGIGLSALEMVEFIGEVWLSLVRL
                     VRGKARFRPSDLGYLIQECGANALPIVSLISVLVGVILAYVGAIQLRLFGAQIYVADL
                     VGLGMAREMGAMMTAIIMAGRTGAAFAAQLGTMQVNEEIDALKTLGISPMEFLVLPRM
                     IALVIMLPPLCLYADLMGILGGALVGIGTLDLSPLLYFNETQSAVELQDFLAGLVKAA
                     VFGALIAVSGCLRGMQCGRSASAVGEAATSAVVTAIVFIIVSDAILTVIYDIIGL"
     assembly_gap    1624..1723
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1973..3052
                     /locus_tag="PLM79_17920"
     CDS             1973..3052
                     /locus_tag="PLM79_17920"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOV88238.1"
                     /translation="MSKKASPTAIGAFIVGTIVLVLLGIVLFGSGKFFKKTLKFVLFF
                     EGSVKGLSVGSPVDFKGVKVGSVSRINVLMDRKDRTVKIAVYIDIDPARITEMTGTKT
                     VREIVGEPGDKTAVEVLIEQGLRAQLDIQSLVTGQLYVLLDFLPDKPARMVGAEDGVV
                     EIPTVPSTLETISKTIDKLPIEELSSKIVAAINGLEQFINSPELKDTMVAVKETAHNA
                     RSLVETVNGEIKPLSAGVKETLADARKLLKGVDTQIQPLLSKANEVVKDTQGFVVEAK
                     KGISPLVDNAVETLKLTQEAVRRADRTLASLEDLTGERSSVRFELTDALKEISSAARS
                     LRTLADYLERHPEALLTGKSRHGER"
     gene            3039..3656
                     /locus_tag="PLM79_17925"
     CDS             3039..3656
                     /locus_tag="PLM79_17925"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HOV88239.1"
                     /translation="MEKDRMWNRLVMPLVMILAGTSFLTGWGCARSQPSRFFMLHSQP
                     GGPVEETGERKVRSLGILPVRFPQYLDRPQIVTRTSRNEVRLAEFDRWAEPLKTNFTQ
                     VLGENLARLLRSDGIVVFPVQKSVEVQTLLSLEVLQFDGSLGGECRLIARWIVYGRDG
                     KTPLTTGRSDLVERVDSSTYDALVSAQSRAVGALSRDIAQSLAQM"
BASE COUNT          729 a         1164 c         1188 g          726 t
ORIGIN      
        1 ccgaggtccc gtccccccgg cgggctaccg accgtcgaat tcgccaaagg cggggtcctt
       61 tacctctccg gaagcgcccg tctgtttccc gtcccgggtc gatgagcggc ccgtctggga
      121 attcacgggg gatcctcgat tccggaccct gggcctggac ggtttctctg gcaacttttc
      181 cggaaccgcg ctagattgac gacgcctttt cgcaggacct caccgggaac gagtgccggt
      241 gctcatggaa ggcggaattc tttcaccgtc ccgggatggg acggagcgga accacggatg
      301 acgcagccgc atgacggatc caacactacg tcgggggaca aggacccccg ggtgatcttc
      361 ctgccccggg acggggacgg gctggtcgtc cggctgaccg ggcattggaa cctcgccgaa
      421 gggcttcttt ccacggattc cgcccgcagg gagatcgagg gccatcccac cctcaagacc
      481 gtgtctttcg aagcctggga cctggccggg tgggacagca gtctgctcag ctacctggtc
      541 caactccacc aggtctgcgt ccaaaaaaac ctcgatgtgg agtgggaccg gctgcccgag
      601 gggttgagaa agctcctggt actggccacg gcggttccgg agagcaaaga agcccgaagg
      661 cgggaaggga ggtcgtccct tttgcggcga atccgcgagg ggctttccgg gatcggcctt
      721 tccgccctcg agatggtgga gttcatcggg gaagtgtggc tgtccctggt tcgcctggtg
      781 cggggcaaag ctcgattccg cccctcggac ctgggatacc tgatccagga atgcggagcc
      841 aacgccctgc ccatcgtttc tctcatcagc gtcctggtcg gagtcattct cgcctatgtg
      901 ggcgccattc agctgaggct tttcggagca cagatttacg tagcggacct ggtaggcctg
      961 gggatggccc gggaaatggg ggccatgatg acggccatca tcatggcggg aagaacgggg
     1021 gcggcctttg cggcccaatt gggcaccatg caggtgaacg aggaaatcga cgccctcaag
     1081 accctgggaa tctcccccat ggaattcctg gtcctgcccc ggatgatcgc cctggtgatc
     1141 atgcttccgc ccctgtgcct gtacgccgac ctcatgggaa tcctgggggg cgccctcgtg
     1201 gggatcggaa ccctggacct ctcccccctg ctctatttta acgaaaccca gagcgccgtg
     1261 gaattgcagg acttcctggc cgggctcgtc aaggccgccg tgttcggggc cctcatcgcc
     1321 gtctccggct gccttcgagg gatgcagtgc ggcagaagtg catcggcggt gggagaagcg
     1381 gcaacctcgg cggtggtgac ggccattgtg ttcatcattg tgtcggatgc aatcctcacg
     1441 gtcatctatg atatcatcgg gctgtaggac cgggtctatg gaagggaagg aatcgccggg
     1501 agaggtcccc atcgaggttc gagacctgac catggcgtat gggagctttg tgatccagcg
     1561 ggacctcagc tttaccgtcc ggcgggggga tatcttcatc atcatgggag ggagcgggtg
     1621 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccattg ccaccaactc
     1741 cgtcttcctg gattcggaaa gcagaaccat catcgcccgg ggggaccccc accggttgct
     1801 cgaagagtcc cgggacccca aggtgttgag tttcctgacg agaggaaaac cctcccgggc
     1861 gctggaaacg ggcctcgctc cgtcgtaagg gggttcccgg gatggtgccc cgaaggtggg
     1921 ctcccgtgcg ggagattcaa acccttcaac caggagagat catcgagccg tcatgagcaa
     1981 aaaagccagt cccacggcca tcggagcctt catcgtgggg accatcgtcc tggtcctgtt
     2041 ggggatcgtt cttttcggaa gcggcaaatt cttcaagaag acgctgaaat ttgtgctctt
     2101 ttttgaaggc tccgtaaagg gcctgtccgt cggatctccc gtggatttta agggagtcaa
     2161 ggtggggagc gtgtcccgca tcaacgtact catggatcgt aaggaccgga cggtcaagat
     2221 tgccgtctat atcgacatcg atcccgcccg catcaccgag atgaccggta ccaagaccgt
     2281 tcgggagatc gtcggggagc cgggagacaa gacggcggtc gaagtcctga tcgaacaggg
     2341 tctgagggcc cagctcgaca ttcagagcct ggttacgggc cagttgtacg tactgctgga
     2401 ctttcttccg gacaagcccg cccgcatggt gggagcggaa gacggggtcg tcgagattcc
     2461 cacggtgccg tccaccctcg agaccatatc caagaccatc gacaagctcc ccatcgagga
     2521 actgagcagc aagatcgtcg ccgccatcaa cggcctcgaa cagttcatca attctcccga
     2581 gctcaaggac accatggttg ccgtcaagga gacggcccac aatgcacgtt ccctggtcga
     2641 gaccgtcaac ggcgagatca agcccctctc ggcgggcgtg aaggagactc ttgccgatgc
     2701 gcgcaagctc ctgaaaggcg tggacactca aatccagccg ctcctgtcca aggccaacga
     2761 ggtggtcaag gacacccagg gttttgtggt cgaggccaag aaaggaatct cccccctggt
     2821 ggacaacgcg gtggaaacgt tgaagctgac ccaggaggcg gtccggaggg ccgatcggac
     2881 cctggcgtcc ctcgaggatt tgaccgggga acgatccagc gttcgcttcg agctcaccga
     2941 cgccctcaaa gagatctcct cggccgcccg gtccttgcgg accctggcgg attatctcga
     3001 aagacacccg gaagcccttc taacgggaaa gagccgacat ggagaaagat agaatgtgga
     3061 atcgtctggt gatgccgttg gtcatgatcc tggcgggcac gagtttcctc acgggatggg
     3121 gatgcgcccg ctcccagcct tctcggttct tcatgctcca ttcccagccc ggaggacccg
     3181 tggaggagac gggcgaaagg aaggttcgtt ccctggggat tctccccgtg cgatttcccc
     3241 agtacctgga tcggcctcaa atcgtcaccc gcacaagtcg aaacgaagtc cggctggccg
     3301 agttcgatcg ctgggccgaa cccctgaaga ccaatttcac ccaggtcctt ggagaaaacc
     3361 tcgcccgcct gctccgctcc gacgggatcg tggtcttccc cgtgcaaaag tccgtggagg
     3421 tccagacact gctgtccctg gaagtgctcc aattcgacgg atcgttggga ggcgagtgtc
     3481 ggctcatcgc ccgatggatc gtctacggca gggacggcaa gacccccttg accacgggaa
     3541 ggtccgacct ggtggaacga gtggactcct cgacgtacga cgctcttgtc tcggctcaaa
     3601 gccgcgccgt aggggccttg agccgcgata tcgcccagtc cctggctcaa atgtagccgc
     3661 cgggcagggc cactgtttcc ccggcctttg tccccgccgt ctctctcctc gatgcctgcc
     3721 aaagtgactg cttccggttg acccgcccga aaggagtccc taacttgacg gcacagtcac
     3781 cgggtccccc atggccgggg ccgccggtgc ggcgctgccc ggttgcgaaa aaccaacttc
     3841 cggctccttc aaggaaagtt ccacaatcac ccaaaactcg cgaggaggtc ctatggcgga
     3901 acgattt
//
LOCUS       DAOOVC010000253         3832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_12957_length_3832_cov_6.350013, whole genome shotgun sequence.
ACCESSION   DAOOVC010000253 DAOOVC010000000
VERSION     DAOOVC010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12957_length_3832_cov_6.350013"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLM79_17930"
     CDS             complement(<1..348)
                     /locus_tag="PLM79_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88240.1"
                     /translation="MKDSYSAEELAAILGISGRAVRRKGMSGGWQFTDMPNGRGGGTR
                     KLYVYAALPKEIRDRIDAHELERVLTEVGRSCAWVGLGPAGNENGSGTPVPVNGNGNG
                     NSGNGTDRAVYSAA"
     gene            complement(577..1017)
                     /locus_tag="PLM79_17935"
     CDS             complement(577..1017)
                     /locus_tag="PLM79_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88241.1"
                     /translation="MKTLLSRQSSNLRSFINELGFGSIASFRKAARALQAYVRERKKA
                     TDPLCEVTVTCGAQWASNHQGYWYLLKANGSEKETAVRELLEHFGLPEWFVLQDGHEK
                     GRLSQLATLVEFGMELPVEHLKRSCDAEGGAAQREPSIETDKEC"
     gene            complement(1038..1250)
                     /locus_tag="PLM79_17940"
     CDS             complement(1038..1250)
                     /locus_tag="PLM79_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88242.1"
                     /translation="MNRDEIRKWMIDEGVTQAQIAREARVSKALVSLVIKGERQNDLV
                     VTLLRGHGCPEEYLAAPRKQKRRKAA"
     gene            complement(1247..1714)
                     /locus_tag="PLM79_17945"
     CDS             complement(1247..1714)
                     /locus_tag="PLM79_17945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88243.1"
                     /translation="MGFNSNSQRKLRYHGRQLTMRDMLVVDPGPHLKAAMSEAIKESD
                     LSREQIVDEMNRLAKICGIACNGKSRQVTAALLDKWVAPGATAYQIPLRLLPLFCRAV
                     GSNLPLEVYASAFIDVRLVSMDEYRMLEWAKAEIEFRLARRKSRKIAQEVGIE"
     gene            2160..2528
                     /locus_tag="PLM79_17950"
     CDS             2160..2528
                     /locus_tag="PLM79_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88244.1"
                     /translation="MVAAEREARFLEQPGGNPEPPGLKISDDLQVAAKVLESGSAYAL
                     ALHLNIQQFGKAVDDQAANRAMREKLDAQAQTLNAQAKTISELQDECAAMKKKIAELE
                     TQLARIIATGGGARSQAEVA"
     gene            2703..3389
                     /locus_tag="PLM79_17955"
     CDS             2703..3389
                     /locus_tag="PLM79_17955"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HOV88245.1"
                     /translation="MRRHLWLVLSLFLFNPANLVADSTESFRCGSMHHKNLVQLGNTE
                     DYVLQSCGEPQSRHYDRWIYDGGRGDFIYTLRFKRGRLVFIDRGDRSLGTRPVSDTSP
                     DPAPQAQAPKKPSIKDVDHVRVFAVDEDGVLRIDISYLNRDRDELVFWENSMARCVCK
                     AYLNKGNLLKPTRGDLIGSVHKDVNRYDQDLYLDISYRYRSKDLWGIIECDVDTRYNQ
                     LHATANFHIK"
     gene            3634..>3832
                     /locus_tag="PLM79_17960"
     CDS             3634..>3832
                     /locus_tag="PLM79_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88246.1"
                     /translation="MRFFALLDLQYLTVSLCLGLIALILVYLSWASYVTRKEAPKDGE
                     DAPGISPGPSLPPHGPGNPVPA"
BASE COUNT          840 a         1139 c          961 g          892 t
ORIGIN      
        1 cgcggccgaa tacacggctc gatccgtccc gtttccgctg ttcccgttcc cgttcccgtt
       61 caccggcacc ggcgtcccgg aaccgttttc gttcccagca ggtccgagac ccacccaggc
      121 gcatgaccgg ccaacctccg taaggacccg ctcgagctcg tgcgcgtcga tccggtcccg
      181 gatttccttg ggcaaggctg cgtagacata cagcttccgg gtcccgccgc cgcgcccgtt
      241 cggcatgtca gtgaactgcc agccgccgga catgcctttc cttcgcactg cgcgcccgct
      301 gatcccaaga attgcggcaa gttcttcggc tgagtaggag tctttcatgg gcgcactctt
      361 tcttgctggt gcacgctgtt cgtgctagct ttggcggaag ttctttcctg cagaacttgc
      421 ttcaatcgat taccggaaca aaagggtggt tctatgggcg agacggacag gaacccggaa
      481 tcgaagttct gggatattgt gctgaagaac gcggaacggg cagcaaatgc agtgggggtt
      541 gcgaccgtcc tgttcaccat cggtgtcctc ggcatttcaa cattccttgt cggtttcaat
      601 cgagggttct cgctgggctg cgccgccttc ggcatcgcag gaacgcttga ggtgctccac
      661 aggcaattcc atgccaaact cgacaagcgt cgccaattgc gagagtctgc ccttttcatg
      721 gccgtcctgg agcacaaacc actctggaag cccaaaatgt tccaggagtt cacgcactgc
      781 ggtttctttt tcagatccgt tggctttgag gagataccaa tacccctggt gattgctggc
      841 ccattgtgcc ccgcatgtca cggtcacctc gcagagagga tcggtcgcct tcttccgttc
      901 gcgtacatac gcctgcagtg cgcgtgcggc ttttctaaaa gatgcaatag aaccaaatcc
      961 gagctcattg atgaagcttc tcagattgct ggattgcctc gatagcaatg tcttcatcac
     1021 agtatcctcg acagcctcta tgccgccttg cgcctctttt gcttccgagg cgcagccagg
     1081 tactcttccg ggcacccgtg cccacggagc agcgtcacga ccaggtcgtt ttgccgctcc
     1141 cccttgatca ccagacttac cagcgccttc gacaccctcg cctccctggc gatctgcgcc
     1201 tgggtcaccc cctcgtcgat catccacttc cttatttcgt ctcggttcat tcgatcccaa
     1261 cctcttgggc gattttcctc gacttccgtc gagccaggcg aaactcgatc tcagccttgg
     1321 cccattcgag catcctgtac tcatccatgc tgactagcct cacatcaata aacgccgacg
     1381 cgtagacctc gagcggcagg ttcgacccaa cagcccggca gaacagcggc aacagcctca
     1441 gtgggatctg gtaggctgtc gcccccggcg caacccactt gtccagcagc gctgccgtga
     1501 cttggcggct ctttccgttg caggcaattc cgcaaatctt cgccagacgg ttcatttcat
     1561 cgacgatttg ttcacgagac aaatccgatt ccttgatggc ttcgctcatg gccgccttca
     1621 ggtgaggccc cggatcgacg accagcatgt cccgcatggt caactgccgt ccgtgatatc
     1681 tgagttttct ctgagaattg ctattgaacc ccacccgagg ctcctgtatt ttgtgaacga
     1741 tgtgtgaact agtttacacc aagtttttcg atatgcaata aaaaaacttg gttccgagca
     1801 agatttttga ttgatacggt tatctatgta ttatttatag gataatcaat atgttgcatg
     1861 cttccgagca ttcaaggacc agttccgagc aaagttccga gcaagggctg tttgctcgga
     1921 aggagtttga ggcccaaatg gaccggattt gggatgctct gggggtcgat aacgatacag
     1981 ctcttgcacg acttttgggg aaggctcagg caactgtctc tgccgccaga acgaataaat
     2041 ctattccaca gtcatggctt aaaatcctta atcagaaata tcgtttgtca cttcaatgga
     2101 ttctattcgg aaccggtcct atgaaggaag gacaggagaa gtcgcaggaa acccaatcta
     2161 tggttgcggc tgaaagggaa gctaggtttc ttgaacaacc tggaggcaac ccagaacccc
     2221 ctggtcttaa gatctctgat gaccttcagg tcgcagccaa ggtcctggaa tcggggtccg
     2281 cctacgccct ggcgctccac ctcaacatcc agcagttcgg aaaggcggtg gacgaccagg
     2341 cggccaaccg ggccatgagg gaaaaactcg acgcacaggc ccaaaccctc aacgcacagg
     2401 ctaagacgat cagcgaactc caggacgaat gtgctgccat gaaaaaaaag atcgcagaat
     2461 tggaaaccca gttggcgagg atcatcgcca ccgggggtgg cgccaggtcc caggcggaag
     2521 tcgcctaaga actacttgcg ccgccgccgg gacaacgtca tctatctcgt cgatccctga
     2581 cccgctactt gggttcgctg gtgcgaaccg actcgaaaag actttacagg atcgcctctt
     2641 tttcccataa tctcgcccat cgttagttac catctgccgt cactcatacc ttggagtcca
     2701 cgatgcggcg tcacctatgg ctcgtccttt ctctctttct tttcaaccca gcaaacttgg
     2761 tggccgactc aaccgagtct ttccgatgcg ggtcaatgca tcacaagaac ctggtacaac
     2821 tcggaaacac cgaagactat gtgctgcaaa gttgcggaga gccacagtcc aggcattatg
     2881 atcgatggat ctacgacgga ggcagggggg attttatcta tacccttcga ttcaaaagag
     2941 gaaggctggt attcatagac cgtggagatc gcagcctcgg cacgcggccg gtctcggata
     3001 cctccccgga tcccgcccca caagcgcagg caccaaagaa gccttccatc aaggatgtgg
     3061 accatgtcag ggtgtttgcc gtcgatgagg acggagtttt gagaatagac atttcctacc
     3121 tcaatcgaga cagagatgaa ctggtctttt gggaaaacag catggccaga tgcgtgtgca
     3181 aagcctattt gaataaaggc aatctactga agccgactcg cggcgatctg atcggtagtg
     3241 tccataagga cgtcaatcgt tacgaccagg acctctatct cgatatctca taccgctacc
     3301 gatccaaaga cctgtggggc attatcgagt gtgatgttga cacacgatac aaccagctgc
     3361 acgccaccgc caacttccac atcaagtaat ccaatgtcca ttttaatgtc catttttcgg
     3421 tgtccaattt tctgagatta cctgcaaaaa aacggcccag accacacttt ttctgagatt
     3481 gcccatcgca ccccagcgtc cggcgtataa ccgcaccagt cctacattcg tcccgcaaaa
     3541 gttcccccaa atcggcctca gtcaattttc tacgattact tgaaagggga tacctccttt
     3601 tgacccttta ctggatgacg aggtgaggga gccatgagat tctttgcgct cctggatttg
     3661 cagtacctga cggtgagcct ctgcctggga ctcatcgccc tcatcctggt ctacctttcc
     3721 tgggcaagct acgtcacccg gaaagaggcc ccgaaagacg gggaagatgc gccggggatt
     3781 tcccctggtc cttcgctgcc cccccacgga cccggcaatc ccgtcccggc ct
//
LOCUS       DAOOVC010000254         3805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13068_length_3805_cov_5.444800, whole genome shotgun sequence.
ACCESSION   DAOOVC010000254 DAOOVC010000000
VERSION     DAOOVC010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13068_length_3805_cov_5.444800"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="selD"
                     /locus_tag="PLM79_17965"
     CDS             complement(<1..912)
                     /gene="selD"
                     /locus_tag="PLM79_17965"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOV88247.1"
                     /translation="MGPTVLSDALRDFPRPSDPDLLIGFETSDDAAVYRLTDSLAVIS
                     TVDFITPPVDDPVWFGRIGAANSLSDVYAMGGRPVMALNLVMFPAKELDTEVLRRILL
                     GGREKVVEAGASLAGGHSVDDREPKYGLAVTGIVSPDRILTNRGARPGDALVLTKPVG
                     SGVLFNGCRSGRLPFRELEAVLPQIAALNRTALETALSYDIHACTDITGFGLLGHALE
                     MATASGVAVDLDFGAVPLYPNVLEMYRKGETTGSNSANRELLRGHLELKAALSREEAE
                     ALVDPQTSGGLLFSLPPDQAEELVERLK"
     gene            1241..1888
                     /locus_tag="PLM79_17970"
     CDS             1241..1888
                     /locus_tag="PLM79_17970"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HOV88248.1"
                     /translation="MSRRIEKVRTDRNEDRLEADLEKYRGKALELGAARAAVIPASEI
                     PVDERVTLKCQIPRCFGYGAGAHCPPNTLKPAELREHLRKYRWAVFFVKDVPSAVIVR
                     DKATIRERVAVYREIYRIVSEIESMAFYDGHYLAFGFGAGSCRHTFCGEEKTCAALEG
                     KRCRHSLSARPSMEAVGIDVYRMVASAGWDIYPIGSGAKPEEIPKGTLAGIVIVQ"
     gene            2040..2816
                     /gene="xth"
                     /locus_tag="PLM79_17975"
     CDS             2040..2816
                     /gene="xth"
                     /locus_tag="PLM79_17975"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOV88249.1"
                     /translation="MSWKLATFNVNGIRARLPIVLAWLEKHRPDVLCLQEIKCRDSEF
                     PGEAFREVGYESTVKGRKSQSGVAVLSRSSPEEVRTHFEEEARLIAASFSGIWVVNTY
                     VPQGRDPQDPAFKAKIDFFKQLKEWFSLHFKPDQPLVWTGDINVAPGPLDVFDPKRME
                     GKVGFHPEERKALADTVSWGFEDVFRIHHPGEKQFTFWDYRIPSSLEKNLGWRLDHIW
                     ATPGLARLSRDCRVDEGPRAMDRPSDHTPVWVEFDLEPRG"
     gene            2928..3590
                     /gene="rpe"
                     /locus_tag="PLM79_17980"
     CDS             2928..3590
                     /gene="rpe"
                     /locus_tag="PLM79_17980"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOV88250.1"
                     /translation="MKKLSASVLSADFGKLAREVQAVEAAGTDWIHVDVMDGHFVPNI
                     TIGPAAVRAIRKAATLPLDVHLMIEDPDRYLGPFVDAGADWLGVHVEACRHLHRTVQR
                     IKELGAKASVALNPATPLVSIESILSDVDMVLLMTVNPGFGGQKLIPSVLTKIRECRR
                     WIDEKGLSAVIEVDGGVHRGTIDDLVEAGVDVYVAGSAVFEGGNYRDNIRVLKERMGR
                     TK"
BASE COUNT          753 a         1133 c         1229 g          690 t
ORIGIN      
        1 cttcaaccgt tccaccagtt cctccgcctg gtccgggggc agggaaaaca gcaggcctcc
       61 cgaggtctgc gggtccacga gggcttccgc ctcttcccgc gacaaggcgg ccttcagttc
      121 caagtgaccc cggagcaact ctcggttggc ggaattgctt cccgtggtct cccccttgcg
      181 gtacatctcg aggacgttgg ggtagagcgg aacggccccg aagtccaggt cgacggcaac
      241 accgcttgcc gtggccatct cgagggcatg ccccaggaga ccgaagccgg tgatgtccgt
      301 acaggcgtga atatcgtagg acagggccgt ctcgagagcc gtccggttga gggccgcgat
      361 ttgaggcaga accgcttcca gttcccggaa cggaagcctt ccggacctgc atccgttgaa
      421 aagaactccc gatccgacgg gtttggtgag aacgagagcg tctcccggtc gtgccccccg
      481 gttggtcagg atgcgatccg gggaaacgat ccccgtgacg gcgaggccgt acttgggctc
      541 ccggtcgtcc accgaatgcc cgcccgccag ggatgccccc gcttccacga ccttctcgcg
      601 gccgcccagg aggatccggc gcagaacctc cgtgtccagt tcttttgccg ggaacatgac
      661 caggttcagg gccataacgg ggcgtccccc catggcgtac acatccgaga gggagttggc
      721 cgcgccgatt cgaccgaacc aaacggggtc gtccacggga ggggtgatga aatccaccgt
      781 ggaaatcacg gccaggctat ccgtcaggcg atagacggct gcatcgtcgc tggtctcgaa
      841 cccgatcagg aggtcgggat ccgaaggccg ggggaaatcc cgaagagcgt ccgacaggac
      901 cgtcggacct atcttggcgg ctcaaccgca ggtccttgcg agtcgagtca gggtcttgtt
      961 cctgccaaaa aagttcatcg catccactcg tagaaggtcc aacgtccgcc cgcatcacga
     1021 cgatgcttcc ctttgggagt tgactcggag ggccggaggg ggaaaacggc ctctcccggg
     1081 atcgatctct ctccccctcg cccggcggcg tgcgaacctg ctgcaagccg catccctcgg
     1141 gcatttgttc cacccattcg gtggatttta ccaccaggat gtgcaaaaat gaaggatagg
     1201 cagagggtcc ttgttgctag atcacacgag gaggatgggg atgtccagga gaattgaaaa
     1261 ggtgcggacg gacaggaacg aagatcgtct cgaggcggac ctggaaaaat accgcggcaa
     1321 ggccttggaa ctgggtgcgg cccgggcggc cgtcatcccc gcgtccgaaa tccccgtgga
     1381 tgagcgcgtg accctcaagt gccaaatccc ccggtgcttc gggtacggtg ccggagccca
     1441 ttgtcccccc aacaccctca aacccgctga gttgcgggaa caccttcgaa agtaccgatg
     1501 ggcggtattc ttcgtcaagg acgttccttc ggcggtcatt gtcagggaca aggcgaccat
     1561 ccgggaaaga gtggccgtct accgggaaat ctacaggatt gtgagcgaaa tcgaatccat
     1621 ggccttctat gacgggcact atcttgcctt cggcttcggg gcgggctcct gcagacacac
     1681 cttctgcggg gaagaaaaga cctgtgccgc cctcgaggga aagcggtgcc gacattcgct
     1741 gtcggcgcgt ccgtccatgg aagccgtggg aatcgatgtg taccggatgg tggcctcggc
     1801 cgggtgggac atctatccca tcggcagcgg ggccaagccc gaagagattc ccaagggaac
     1861 tctggcgggc attgtcatcg tccaatagcc tcccgggaag gcttccgggg ttcccgaaag
     1921 ccccgggggc gaagaggcgt cccggagagt cctcggggat tgttcggcaa gggaaggaag
     1981 cctggaattg cgggtttcgc aagggtatag ggtgggcctt gaacccggga gggatcccag
     2041 tgagttggaa acttgcgacc tttaacgtca acggcattcg ggcccgtctg cccattgttc
     2101 tggcctggct ggagaagcat cgtcccgacg tgctttgcct gcaggaaatc aagtgccggg
     2161 actcggaatt ccccggggaa gccttccggg aagtcggata tgaatccacg gtaaagggac
     2221 gcaaatccca aagcggggtt gccgtcttga gtcggagttc ccccgaagag gtgaggaccc
     2281 atttcgagga ggaagcccgg ctgatcgcgg cgtctttctc ggggatctgg gtggtcaaca
     2341 cctatgtgcc ccaggggcgc gacccccagg acccggcctt caaggccaag atcgactttt
     2401 tcaaacaact gaaggaatgg ttttccctcc atttcaagcc cgatcagccc ctggtctgga
     2461 ccggggacat caacgtggct ccgggacccc tggatgtgtt cgatcccaaa aggatggagg
     2521 gaaaagtcgg gttccacccg gaagagcgca aagccctggc ggacacggta tcctggggat
     2581 tcgaggatgt gttccggatt caccacccgg gggaaaagca gtttacgttc tgggactacc
     2641 ggattccctc gagcctggaa aagaacctgg gctggcgcct ggaccacatc tgggcaaccc
     2701 ccggcctggc ccgactctcc cgggactgcc gggtggatga aggaccccga gccatggatc
     2761 gacccagcga tcataccccg gtctgggtcg agtttgacct ggaacctcga ggctagggaa
     2821 agcggctccc gaggggggcg tcggcccgga tgccggatcg accgcgattc ccggtcggac
     2881 gggaaaaccg cctgcgaccg ggcactctct tccatgcagg agaattcatg aaaaaattgt
     2941 ccgcatccgt cctgtcggcg gatttcggga agctcgcccg ggaggttcag gccgtggaag
     3001 cggccggtac ggattggatt cacgtggatg tgatggacgg gcacttcgtt cccaacatca
     3061 ccatcggacc tgcggcggtt cgggccattc gcaaggccgc cacccttccc ctggacgtgc
     3121 acctcatgat cgaagacccg gatcggtacc tggggccgtt cgtggatgcc ggggccgact
     3181 ggctgggagt ccacgtggag gcctgcaggc acctccaccg caccgttcag cgaatcaagg
     3241 aactgggggc caaggcctcg gtggccctga acccggccac gccgttggtt tccatcgaat
     3301 ccatcttgtc ggatgtggac atggtcctcc tcatgacggt gaaccccggc ttcgggggac
     3361 aaaaactcat cccttcggtg ctgaccaaaa tccgagaatg tcgccggtgg atcgatgaga
     3421 aagggttgtc ggccgtcatc gaggtggacg gaggagtcca ccggggtacc atcgacgacc
     3481 tggtggaggc cggggtggac gtgtacgtgg cgggttccgc cgtgtttgag ggcggcaact
     3541 atcgggacaa catccgggtt ctaaaggaaa gaatggggcg aacgaaatga aaggaaggaa
     3601 ttcccgaggt tccgaggtaa ggaggcggga aaccccgccc tccccaaggg ccccttagcc
     3661 cttcttgagg aagattccca gcatccccgc caacttcagc tcccgggccg ccttgacctc
     3721 gaaccggcac tcctccaggg cttcgtgcag gtctccacta aagaagcggt cttctttggg
     3781 gtgcaggagg atgtgccggg tgatg
//
LOCUS       DAOOVC010000255         3803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13077_length_3803_cov_6.601387, whole genome shotgun sequence.
ACCESSION   DAOOVC010000255 DAOOVC010000000
VERSION     DAOOVC010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13077_length_3803_cov_6.601387"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1721)
                     /locus_tag="PLM79_17985"
     CDS             complement(144..1721)
                     /locus_tag="PLM79_17985"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HOV88251.1"
                     /translation="MAEIVSEKEFKDRKSLLEAKETLSPQEERFERWRNTIGLVLGPA
                     VGLMLYLMPMPSLSPQAHKLAAIIGWVGVWWITEPIPIPMSALMGAVLCVLFGIADVK
                     KVFAPFADPIIYLFMGGFMLAEAMAIHGLDKRFAYAIMSMKAVGNSTGRILAAFGVIC
                     GFLSMWISNTASTAMMFPIALGIVYAMADIVGQQSGTGMDPTKLRFGTGMMLMAAYGA
                     SAGGIGTPVGTPPNLIGIAMIEKFCHVKIAFFQWMSFAVPLVILLFVLLYVLMYYLHK
                     PEVEHISGSREYVAQERMQLGKWTQGQKNAMFAFLVTVTLWLIPGVLAILYGTDAPIS
                     KTYGRYVPEGVAALIGATLLFLLPVNWREREFTLSWGQAVKIDWGTLLLFGGGITLGN
                     LMFELKLAEVIGKGLLGLSGASSVWGITFGAVFIAIIVSETSSNTASANMVVPVMISL
                     AQAAGVNPIPPAIGATLGASWGFMLPVSTPPNAIVYGSGMVPITKMIRAGIFFDILGG
                     LLIWVGLRILLPLVGLA"
     gene            complement(1792..2337)
                     /locus_tag="PLM79_17990"
     CDS             complement(1792..2337)
                     /locus_tag="PLM79_17990"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOV88252.1"
                     /translation="MKARDLMIPASDCARVAEDRTIYDAVVMLEAWRRRCQSEYRPRL
                     VLVYDKDFKIIGSAGHAGMLRALSDAPADLSGEPSAASSSHDTTLPPFPPELTTAWGK
                     VLTRLHTAAHYVRVKEAMIHYEESQYIEDDTPMEVVFRRLVSGPYESLVVVSGRATIG
                     IVRLSDVFSKVCKEISRSGMK"
     gene            2846..>3803
                     /locus_tag="PLM79_17995"
     CDS             2846..>3803
                     /locus_tag="PLM79_17995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV88253.1"
                     /translation="MKKLTVRPGIKPVRWVRDMHSKAFGIPTRTEVRGKCPDAGFRRD
                     GGTLVALFLGTLLSALAFMVAGAAGIAAATGQERAPIKVGVVLPLTGAQREFGEMEKN
                     SFLMGLEEINAAGGVNGRPIELLIEDDRSRIEVGRAAAEKLILQDQVIALTGGYSSDV
                     TFAVAAVAQYRKTPFVITTGSADEITQQGAEYVFRLNQPVSRYATALVEFLQEVVKPK
                     SVAVLYENSLFGRSGSNMFVDLAIEAGWQVLMKESFEPGTQDYKPLLTRTRTLKPDVV
                     YVIAYVKEAAALVQQAKEIRLTPKVFAGAAAGFTLPEFRTLAG"
BASE COUNT          842 a         1137 c         1112 g          712 t
ORIGIN      
        1 agtttgccgc accccagagg acaatgccgc gttcctgcat ccattggatc agccaagacg
       61 gacgggacgc gaagggcgaa gacaagaaaa tcctccttgg gatcgggcat ccggcggtca
      121 cgaacggctc ggacaagtcg atttcaggcc agtcccacca ggggcagcaa gatccggagc
      181 ccgacccaga tgaggagacc tcccaggata tcgaagaata ttcccgcccg gatcatcttc
      241 gtaatgggaa ccatgcccga cccgtagacg atggcgttgg gcggtgtgga cacggggagc
      301 ataaaacccc agctcgcacc cagggtggcg ccgatggcag gagggatggg attgaccccc
      361 gccgcctggg ccagggaaat catcaccggc accaccatgt tggccgaagc ggtgttcgac
      421 gaggtttcac tcacgatgat ggcaatgaag acggccccga aagtaatgcc ccacacggag
      481 gatgctccgc tcagccccag gaggcccttg ccgatgacct ccgccaactt cagctcgaac
      541 atgagatttc ccagggtgat tccgcccccg aagagcaaga gggttcccca gtcgatcttg
      601 accgcctggc cccaggagag ggtgaattcc cgttctctcc aattgaccgg gaggaggaac
      661 agcaaggtcg cgccgatgag tgcggccacg ccttcgggca cgtatcttcc gtaggtcttg
      721 gaaatcgggg catcggtccc gtaaagaatg gccagcacgc cgggaatcag ccagagggtc
      781 acggtgacca ggaacgcgaa catggcattc ttctgtccct gggtccattt gcccagctgc
      841 atgcgttcct gggccacgta ttcccggctc cccgagatat gctccacctc cggcttgtgc
      901 aggtagtaca tgagaacata gagcaggaca aacaacagga tcaccagggg aacggcaaag
      961 gacatccatt gaaagaaggc gatcttgacg tggcagaact tctcgatcat ggcgatgccg
     1021 atgaggttcg gcggggtccc gacgggagtg ccgatccctc cggcggaagc cccgtaagcc
     1081 gccatgagca tcatgcccgt gccgaatcgc agcttggtcg gatccatgcc ggttccgctt
     1141 tgctgcccca caatgtccgc catggcatac acgatgccca gggcaatggg aaacatcatg
     1201 gccgttgagg cggtattgct gatccacatg gaaagaaaac cgcagatgac cccaaaggct
     1261 gccaggattc tccccgtgct gtttcccacc gccttcatgg acatgatggc ataggcgaat
     1321 cgcttgtcca acccgtgaat ggccatggct tcggccagca tgaagccgcc catgaaaagg
     1381 tagatgatcg ggtccgcaaa gggcgcgaac accttcttga cgtcggcaat tccgaaaaga
     1441 acgcacagga cggcccccat caaagcgctc atgggaatgg gaatgggctc cgtgatccac
     1501 cacacgccca cccaaccgat gatcgccgcc agtttatgag cttgtggact cagggagggc
     1561 atcggcatca ggtacagcat gaggcccact gcagggccga gaaccaaacc gatggtgttg
     1621 cgccagcgtt cgaaacgttc ctcctggggc gaaagagttt ccttggcctc caagaggctc
     1681 ttcctgtctt tgaactcctt ttccgataca atttccgcca ttgggttccc tccttcaaga
     1741 tccgagatcc gctatcgagt tcgtccgcca ccgcatcaga gggatgaggg cttacttcat
     1801 tccggaccga cttatctcct tgcatacttt gctgaataca tcgctgaggc gcacaatgcc
     1861 gatggttgcc ctcccggaca ccaccaccag gctctcgtag gggcccgaga ccaaccgcct
     1921 gaacaccact tccatggggg tatcgtcctc gatgtattgg ctctcctcgt agtgaatcat
     1981 ggcttccttc acccggacgt aatgggcggc ggtgtgaagg cgggtcagga ccttccccca
     2041 cgccgtggtg agctcgggag gaaacggagg cagcgtggtg tcgtgagaac tcgaggcggc
     2101 ggaaggctcg cccgacaaat ccgccggggc gtccgacaag gcccgcagca tgcccgcatg
     2161 acccgcgctg ccgatgatct tgaaatcctt gtcgtacacc agcaccagtc gtggtcggta
     2221 ttccgactgg cagcggcgac gccaggcctc gagcatcacc acggcatcgt agatggttct
     2281 gtcctcggca acccgagcgc agtcggacgc cggaatcatc aagtcccttg ctttcatgcg
     2341 cactctcctg gaacggggaa caatggaaac ctcaaacgga gtggtcggcg ctcagatgtt
     2401 agcggcaacc caattccttc ggcaacctcc ggaatgggaa cacggactcg acgggtgcaa
     2461 ccccactcaa aacaaccact cgatctctca ggccaacagc gtactgccaa caaccaatcg
     2521 ctccattcgg gcaaaggttt ggtcgagccc accgccggag tctaatccgg gaggattcag
     2581 gcagcaagca ggaattcagc tttctgcgcc tgtcctcggg acccatgccc gtaggggccg
     2641 gggcaacgaa gaagcgttcg ccaagtcaag ttcatggaat aacctgaaaa atgcatcacg
     2701 gaacttttgg ggacccgccg agaccttttc gggtttctcc gccgattccg atccatgctt
     2761 ttctttgaca gggagcgcac ggagaaccta aaatcattcc aggaaacgat cgaggccaat
     2821 ttccccaacg cgagaggagc gaccaatgaa gaagctgacc gttcggccag ggatcaaacc
     2881 cgtcagatgg gtccgcgata tgcactcgaa agcgttcggc attccgacaa gaacggaagt
     2941 ccggggcaaa tgcccggatg ccggttttcg ccgcgacggc gggacgttgg tcgctttgtt
     3001 tctggggacc ctgctcagcg cattggcatt catggttgcg ggagctgcgg gcatcgccgc
     3061 ggcgacggga caggagagag cacccatcaa ggtcggcgtc gtccttccct tgacgggagc
     3121 gcagcgggaa tttggcgaga tggagaagaa ctcctttctc atgggcttgg aagagatcaa
     3181 tgctgcgggc ggcgtaaacg gccggcccat cgagttgctg attgaagatg accgttcgag
     3241 gatcgaggtg ggaagagccg ccgcagagaa gctcatcctc caagaccaag tgatcgcttt
     3301 gacgggcggc tattcatccg atgtaacttt cgccgtggcc gccgtggccc agtatcggaa
     3361 aacccctttt gtgatcacca cgggatctgc ggacgagatc acccaacagg gagccgaata
     3421 cgtgtttcgc ctgaatcagc ccgtaagccg gtacgccacg gccctcgtcg aatttcttca
     3481 ggaagtcgtt aagcccaaga gtgtggcggt attgtacgag aacagtcttt ttggccggtc
     3541 cggttcgaac atgtttgtcg acctggcgat cgaggcgggt tggcaagtgc tcatgaaaga
     3601 gagctttgaa cccgggaccc aggattacaa gcctcttctc acccgaaccc ggaccctcaa
     3661 gcccgacgta gtgtacgtca tcgcctacgt caaggaagcc gcagctctgg tacagcaggc
     3721 caaagagatc cgattgactc ccaaggtttt cgccggtgcg gctgccggct ttaccctgcc
     3781 cgagtttcgc acccttgcgg gcc
//
LOCUS       DAOOVC010000256         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13102_length_3797_cov_3.384019, whole genome shotgun sequence.
ACCESSION   DAOOVC010000256 DAOOVC010000000
VERSION     DAOOVC010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13102_length_3797_cov_3.384019"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..362
                     /locus_tag="PLM79_18000"
     CDS             207..362
                     /locus_tag="PLM79_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88254.1"
                     /translation="MAHVKSCGLDKEPRFDHGSEACDSFRCRKCEDGEGKVTVDPGRE
                     RDFVDWL"
     gene            complement(604..2367)
                     /locus_tag="PLM79_18005"
     CDS             complement(604..2367)
                     /locus_tag="PLM79_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88255.1"
                     /translation="METRRCLVTCCLVVMILGLPPIFQCRDNPSAALSGEAGFWEIEP
                     VSFSFQNEAKTLRFRSSTIRISYTFHPADRNSGSKPLFVFFNGGPGCATSTGLLSLDT
                     SPYTLNKERTGDRWLKQNPYRWTALGNLLYLDAPNSGFSYNLTHHASDPRQRADEFKV
                     RNFNPFLDAAQCTRLLLRFLAAHPTLQANPVVLVGESYGGIRATAMLNLLLFYPSYGD
                     GSKIYRDKALVDEIQLHLNQVYPSQAGRPMSPSTVANQFGRQVLIEPLLAGRYQVQIA
                     GEMLERKGSPIYRIAAETGKTFTPCRKVEPGSAADRCNPYGNALKFVRSAGRDVYHFA
                     KPANWFEELGNRAGDGLLHARVFSRTLGEEARSIVELAPGAREEAYRYKKNAAADLES
                     VLKGPAFDRLPPGERMCIESRARRLESGREVSRPGDETGHGGQAGRPSDLLVDVLGPL
                     NPWDDYLVGCNDAALETFYVNSAVKAGFPIEPTSLLYGRLFLQNLALVKTFITDAAYD
                     LVIYAPALPASLAKYRAIVQDATVHDEHIVVNYKPGSLPDIPTPLLAVITFPHYDRAG
                     HGVAASQPKKLRDDVGKWLSR"
     gene            2568..2819
                     /locus_tag="PLM79_18010"
     CDS             2568..2819
                     /locus_tag="PLM79_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88256.1"
                     /translation="MIIDLGDGRSVDTQKDLSAAERHILQKLFIWEAMARSVEEFRES
                     RVRAFRLGWNNSGPVAESAVMTAILKDLEQKVVARLRVQ"
     gene            3174..3551
                     /locus_tag="PLM79_18015"
     CDS             3174..3551
                     /locus_tag="PLM79_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HOV88257.1"
                     /translation="MTPKETPKNPGKEQILSGFGKEREKAYREQAMKLFPKICARCGR
                     EFSGKRLRELTVHHKDHNHNNNPPDGSNWELLCLFCHDHEHSKYLDAEWLDEETPRDR
                     REPTSTYQPFAALGSLLKKKEEA"
BASE COUNT          731 a         1080 c         1271 g          715 t
ORIGIN      
        1 tcttggggtc tccctcctgt cgggatcgag tgtcctcgat ccttcccggc ggtagcggct
       61 caagaccgag gccgactacg attttgcttg ggtggaggtc ggcaggaacg gaggaaatcc
      121 tggttgagcg attggctgat cggtgtggtt gcagccaagc ctgcttgaag agcgcacgtg
      181 ggggggcgct ggaaatgggg gatgcgatgg cgcacgtaaa atcttgcggg ttggacaaag
      241 aacctcgctt cgatcacggg agcgaggcgt gcgattcgtt tcgctgcaga aagtgcgaag
      301 acggcgaggg gaaggttacg gtggatcccg gcagggagag ggacttcgtg gattggctct
      361 agtgccggga ggtcgcctgc cttcctcttc aggcggcaga aaaaggaccc gggaattccc
      421 cgggtccttt cgttttgggg tcccgcacac aggggacgtt ccccctcgcc gggctcctcc
      481 catggatggg ggaagcaagt atcgtctgcc ccccgggcgc aacgggccgg ggggagggga
      541 aacctctctc gggccatccg tcctgatcgc ccgctccgcc ggttccgccc caaacccggg
      601 actttaccgg ctcaaccact tgccgacgtc gtcgcgaagt ttcttgggct gtgacgcggc
      661 gactccatga cccgcccggt cgtagtgggg aaaggtgatc acggccagaa ggggagtggg
      721 aatgtcggga agcgagcccg gcttgtagtt gaccacaatg tgctcgtcat ggaccgttgc
      781 atcctgaacg attgctcgat attttgccag ggaggcgggc agggccggag cgtagatgac
      841 caggtcgtaa gcggcgtccg tgatgaaggt cttcaccagg gccaggtttt gaaggaacag
      901 ccggccgtag agcaacgaag tgggttcgat gggaaacccg gccttgacgg cgctgtttac
      961 gtaaaaggtc tcgagggccg cgtcattgca gcctaccagg taatcgtccc aagggttgag
     1021 cgggcccaag acgtccacga gaaggtcgct tgggcggccc gcctgtcccc cgtgcccggt
     1081 ttcgtcgccg ggccgggaaa cctcccggcc ggactccaat cgtcgggctc ggctctcgat
     1141 gcacattctc tccccgggcg gcaaccgatc aaaggccggg cccttcagga cggattccag
     1201 gtcggccgcg gcgttctttt tgtagcgata cgcctcctct cgggccccag gcgccagctc
     1261 gacaatggat cgagcctctt cccccagggt ccgggaaaag acacgggcgt gaagaaggcc
     1321 gtcccccgcc cggtttccca gctcttcaaa ccagtttgcg ggcttggcga agtggtacac
     1381 gtccctgccc gcgcttcgga cgaatttgag ggcgttgccg tacgggttgc agcggtcggc
     1441 cgcggacccc ggctccactt tgcggcaggg ggtaaaggtc ttcccggttt cggcggcgat
     1501 ccgatagatg ggggagcctt ttctctcgag catttccccg gcaatttgga cctggtatct
     1561 tcccgccaac aggggttcga tgagcacctg gcggccgaat tgattcgcca ccgtcgaggg
     1621 ggacatcggc cggccggcct gcgaggggta gacctggttc aggtggagct ggatttcatc
     1681 gaccagggcc ttgtcacggt agatcttgct cccgtccccg tagctggggt agaagagcag
     1741 gagattgagc atggccgtgg ctcgaattcc tccgtagctt tcccccacga ggaccacggg
     1801 attggcttgg agggtgggat gtgcggccag aaaacggaga agcagccggg tgcactgagc
     1861 cgcgtccagg aaggggttga aattccggac cttgaattcg tcggcccgct gccgggggtc
     1921 cgaggcatgg tgggtcaggt tgtaggaaaa gccgctattg ggggcatcga ggtagagaag
     1981 gtttcccagg gccgtccatc tgtagggatt ttgcttgagc cagcggtcgc cggttctctc
     2041 cttgttcagg gtgtaggggg aggtgtcgag actgaggagc ccggtggagg tggcgcaccc
     2101 gggacctccg ttgaaaaaga cgaagagggg cttggatccg gaattccggt cggcggggtg
     2161 gaaggtgtag gagatacgga tggtcgaaga ccgaaagcgc agagtcttgg cctcgttttg
     2221 gaagctgaag gaaacgggtt cgatttccca aaacccggct tccccggaca gagcggccga
     2281 cgggttgtcc cgacattgga agattggcgg gaggcctaag atcatcacca ccaggcaaca
     2341 ggttaccagg catctacgag tctccatctg cttcctttcc gctggatcga gcgattcgac
     2401 cgggacgaat ccctttggcc gttcccgcgc aggggggatc cggaaagagc gtgcctactc
     2461 accggatgcc cgccttcgcg ggcatgacgc ccggatgctc tcggaacccc tcaacgcaag
     2521 tttccatctc cgtcggttga atcacttcca ttggagggtg gaattttatg atcatcgacc
     2581 tgggggacgg ccggtccgtc gatacgcaaa aagatctttc tgcggcagaa cgacacattc
     2641 tgcagaaact gttcatctgg gaagccatgg cccgtagcgt cgaggaattc agggagagca
     2701 gagtccgggc cttccggctg ggatggaaca attccggtcc cgtggcggaa agcgccgtga
     2761 tgaccgctat cctgaaagac ctggaacaga aagtggtcgc ccgcctccgg gtccaatgaa
     2821 accgcgggaa ggggtccgac ctcccggggt gcgcgtcgcc ccgcggttcg gtcccggggc
     2881 tggggctgcc tccgggcgag cctgagtccg cggatcccgg ccctcccgct gcgcggaagt
     2941 tttcgccgct catcgcgctc gggacccgtt gccgcccaca cccatggccg ggaggtcttc
     3001 caacacctct tgatacggcc ttattcgagg agaagtcaac gacttccgcg gagaagccga
     3061 ccgagaaaag ccgagaaagc ccgggccaac gcctccatga atgccgggtc tcctggccca
     3121 agaccgggcg ttctgcccgg gccttccccc tcgaaaaacg gaaaggagca ctcatgaccc
     3181 ccaaggaaac tccaaagaac cccggcaagg aacaaatcct ttccggattc gggaaagaac
     3241 gggagaaggc ctaccgggag caggccatga aactgtttcc caagatctgt gcccgatgcg
     3301 ggcgggaatt ttctgggaag cggcttcgtg aattgacggt gcatcacaaa gaccacaacc
     3361 acaacaacaa tcctccggac ggcagcaact gggaattgct ctgcctgttt tgccacgatc
     3421 atgaacattc caagtacctg gatgccgagt ggcttgacga ggaaactccc cgagacaggc
     3481 gggaacccac ctccacgtac cagcccttcg ccgcactcgg gtctttgctc aagaagaaag
     3541 aagaggcgtg attcttctcg ggtaggcatt gggtttcggg cgcagggcgc gcaaatcggg
     3601 attcgaggaa gagcggagct ctctcgtggc ggttttccta cgaacagggc atgcgcgtcg
     3661 tccaggtgga ggaatcctgt gaacccgggc cggagcgggg tcgtggagga gactcgaccg
     3721 gaggtgggga agggtcctcg tgtctttcct tgaagggccc ggggaagtct gagaaggtca
     3781 tgaaagcgca ccggaat
//
LOCUS       DAOOVC010000257         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13317_length_3740_cov_5.096065, whole genome shotgun sequence.
ACCESSION   DAOOVC010000257 DAOOVC010000000
VERSION     DAOOVC010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13317_length_3740_cov_5.096065"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="PLM79_18020"
     CDS             complement(<1..738)
                     /locus_tag="PLM79_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88258.1"
                     /translation="MEIFPAQAVFGARIRVLLSLLVTVVLVGFPAICPADSVDALYDQ
                     ARKEYSAALVVSSKDQKVQALKQAASQFKKVLKEDSAGRYRDKAHYMIGQCNHHLYDL
                     TKDRAMQKAAIEQYRTVTQKSPNSPLADDAQYLTAMVYLDDDPSQAYLEFAKVGLFYP
                     KGDMKSRAEQKAALLRKTLGCDRGKKSGGEKTGDSAAPPASSGKKVSVATVPPGGGTA
                     GTAGKPAGPSPGTTGASTPPSRATAPPP"
     gene            complement(864..3707)
                     /gene="mutS"
                     /locus_tag="PLM79_18025"
     CDS             complement(864..3707)
                     /gene="mutS"
                     /locus_tag="PLM79_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOV88259.1"
                     /translation="MNTRTPMMQQYLEMKARHPDALLLYRMGDFYELFMEDAVLASKV
                     LEIALTSRDKQAENPVPMCGVPYHAAENYISRLIDAGHKVAVCDQVEDPKKAKGLVRR
                     EITRVITPGLLLESRNLDAAQPNYLAAVASHAKGKSYGLSYLDVSTGDFRAVEVETEE
                     TLLEELLRISPKELLLDQSSRPSWLDRALQILPMRITRLDSADFDERRGYERLLRRLK
                     VHSLDGFGMEGMRWAIRAAGAVLAYLEANLLGACEHIGRLIPYRRADYMVLDESTVRN
                     LEIFQSSNSPGNKTSLLGVLDRTRTPMGARKLHHWLRYPLLDLPRIRARQQAVAELVE
                     QTSLRGQILAELDRMGDMERLNGRNSAGMSSPRDLVALKRCLQVLPAIGELLSRRHSL
                     PHLCRHSSAPGIEGQTGAAEGTPPGRSPRPYLETPGEESSPSDGRILCELALHWDPLQ
                     DVADRIEEILTDPPPASMASGGIIRPGVDAQLDHLVRISRDAKSWMAEYEALERQRTG
                     ISSLKVRYNKVFGYYLEVSRSNLAAVPDNYVRKQTLVNAERFVTEELKKFETEILEAD
                     EKRQEIEERLFGELCRAVASQSSRINAMAERTARLDCLATLADLASKGNYCCPQLDHG
                     DRIFIREGRHPVIEHCIPDGTFVPNDLVLDHEERQVLIITGPNMSGKSTILRQAALIV
                     LMAQIGSFVPAAEARIGIVDRIFTRIGASDDLARGRSTFMVEMQETANILHQATSRSL
                     VILDEIGRGTSTFDGLSIAWAVAECLHDFENTGIKTLFATHYHELTELSRTRARVKNY
                     NVAVKEWQGEILFFHKLLPGAANRSYGIQVAQLAGMPGGVTRRAKEILTQLERGETSL
                     MRERPAAAKGGASRRNAPTPVGRQLHMFDSSLEWLREQILSLDPDHLTPMAALQKLVE
                     MRERIVSLTGSRFRPRQSALDADGVITSTGAKS"
BASE COUNT          633 a         1122 c         1228 g          757 t
ORIGIN      
        1 gggggggggc gccgtcgcgc gggatggggg cgtcgaggcc ccggtcgtcc ccggggaagg
       61 ccccgccggt ttccctgcag tcccggccgt tcccccgccg gggggaaccg tggcgacact
      121 cactttcttg cccgacgatg ccggaggggc ggccgaatcc ccggtcttct ctcccccgga
      181 ctttttcccc cgatcgcacc ccagggtttt tctcaacagg gcggcttttt gctcggcccg
      241 ggacttcatg tcccccttgg ggtagaaaag gcccactttg gcgaactcga ggtaggcctg
      301 ggacgggtcg tcgtccaggt acaccatggc cgtgaggtat tgggcgtcgt cggccagggg
      361 actgttggga gacttttggg tcacggtccg gtattgttcg atggcggcct tctgcatggc
      421 ccgatccttg gtcaggtcgt acaggtggtg gttgcactgg ccgatcatgt aatgggcctt
      481 gtcccggtac ctgcccgcgg agtcctcctt gaggaccttt ttgaattggg aggcggcctg
      541 tttgagagcc tggaccttct gatccttgga agacacgacc agggccgccg aatattcctt
      601 cctggcctga tcgtagaggg catccacgga atcggccggg cagatcgcag gaaaccccac
      661 gagcacaacc gtcacaagca ggctcagcag gacccgaatc cgtgcgccga aaacggcttg
      721 ggccggaaag atttccatga tgtgtccgag agggccgatc cgccgggccc aaatcccgcg
      781 ccggggatcg tgccggggca cccacccatt cggacggctc ggctcatcgg ggccccccgg
      841 ggccggaagc gcggtcaagg ttctcatgat ttcgcacctg tggaagtgat gacgccgtcc
      901 gcatcgaggg cgctttggcg gggcctgaat cgagatcccg tcaggctcac gatgcgctcc
      961 ctcatctcca ccagtttctg cagggcggcc atgggagtca agtgatcggg gtccagggaa
     1021 aggatctgct cccgaagcca ttccagagac gaatcgaaca tgtgcagctg gcgccccacc
     1081 ggggtgggcg catttctgcg gcttgcgcct ccctttgccg cggccggacg ttccctcatg
     1141 agggaagtct ctcctcgctc gagctgcgtc aagatctcct tggcgcgccg ggtaacgccc
     1201 ccgggcattc ccgccagttg tgccacctgg attccgtagg agcggttggc ggctccgggc
     1261 aagagcttat ggaagaagag aatctccccc tgccattcct tcaccgccac attgtagttt
     1321 tttacccgcg cacgggtgcg actcagttcg gtcaattcgt ggtaatgggt ggcgaagagc
     1381 gtttttatac ctgtattttc aaagtcgtgc aaacattccg ccacggccca ggcgatgctg
     1441 agcccgtcga aggtgctggt tccccgcccg atctcgtcca ggatgacaag gctcctggag
     1501 gtggcctggt gcaggatgtt ggccgtttcc tgcatttcca ccatgaaggt ggatcggccc
     1561 cgggccaggt cgtccgaggc gccgatccgg gtaaagatgc ggtccacgat gccgattctt
     1621 gcctccgccg cgggcacaaa ggaaccgatt tgcgccatca aaacgatgag ggccgcctgc
     1681 ctcaggatgg tggattttcc ggacatgttc gggccggtaa tgatcaatac ctggcgctct
     1741 tcatgatcca ggacgaggtc gttgggaaca aaagtgccgt cggggatgca gtgctcgatg
     1801 acgggatggc gcccctcccg gatgaagatc cggtccccgt gatccagctg ggggcaacag
     1861 tagttcccct tggatgcgag atcggcgagg gtggccaggc agtcgaggcg agccgttcgt
     1921 tcggccatgg cgttgatccg ggaggactgg gaggccacgg cacggcacag ctctccaaac
     1981 agccgctcct cgatctcctg ccgcttctcg tccgcctcca ggatctccgt ctcgaacttt
     2041 ttcagttcct cggtgacgaa cctctcggcg ttcaccaggg tctgtttccg gacatagttg
     2101 tcgggaacgg cggccaggtt ggaccgggag acttcgaggt aatagccgaa gactttgttg
     2161 tagcggactt tgagggaaga gatccccgtg cgctgtcgtt ccagcgcctc gtattcggcc
     2221 atccagctct tggcgtcgcg actgatgcga accaggtgat ccagctgggc atcgaccccg
     2281 ggacggatga tccctcccga agccatgctt gcgggagggg ggtccgtgag aatttcttcg
     2341 atacgatcgg ccacgtcttg aaggggatcc cagtggaggg ccagttcgca caagatccgc
     2401 ccgtcggaag gggaggattc ctccccgggg gtctcgaggt agggtcgagg ggatctgccg
     2461 ggtggcgtcc cttccgccgc cccggtttgc ccttcgatgc cgggggccga agaatgacgg
     2521 caaaggtgag gaaggctgtg ccggcgggac aaaagctccc cgattgcggg caaaacctgc
     2581 aggcatcgct tcagggccac caggtcccgg ggagaggaca ttcccgcgct gtttcgcccg
     2641 tttagacgct ccatgtctcc catgcgatcg agttcggcga ggatctgccc ccggagggag
     2701 gtctgctcga ccagttcggc gacagcttgc tgccttgccc ggatccgcgg caggtccagg
     2761 aggggatagc ggagccagtg gtgcagcttt cttgccccca tgggcgttcg ggtccggtcc
     2821 agcacaccca gcagggaggt cttgttccct ggagagttcg aggattggaa gatctcgagg
     2881 ttcctcaccg tggattcgtc cagcaccatg tagtccgccc gccgataggg aatgagcctt
     2941 ccaatgtgtt cgcaggcacc caggaggttg gcctcgagat aggccaggac ggccccggcg
     3001 gcccggatgg cccacctcat tccttccatg ccgaagccgt ccagggaatg gaccttgagg
     3061 cggcgaagca gcctttcgta accccgccgt tcgtcaaagt ccgccgaatc caggcgggtg
     3121 atcctcatgg gaaggatttg cagcgcccgg tccagccagg aaggccgaga actctggtcc
     3181 aacaggagct ctttggggga gatgcgcagg agctcctcga gaagcgtttc ttccgtttcg
     3241 acctcgacgg ccctgaaatc ccccgtggag acatcgaggt aggacaggcc gtaggatttg
     3301 cctttcgcat gagatgccac ggcggccagg tagttgggtt gagcggcgtc caggtttcgg
     3361 ctttcgagga gaaggccggg ggtgatgacc cgggtgattt cccttcgcac caggcccttg
     3421 gccttcttgg ggtcctcgac ctggtcgcag accgccacct tgtgacccgc atcgatcagc
     3481 cgggagatgt agttttccgc cgcatggtat gggaccccgc acatggggac ggggttttcc
     3541 gcctgcttgt cccgggaggt gagggcaatt tcgaggacct tggaagcgag gacggcatct
     3601 tccatgaaca gctcgtaaaa atcccccatg cggtacagga gaagggcatc cggatggcgc
     3661 gccttcatct ccaggtactg ctgcatcatc ggagttcggg tgttcatttc gctccgtgtt
     3721 ccggaccggg tggagtgtgg
//
LOCUS       DAOOVC010000258         3730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13353_length_3730_cov_4.179864, whole genome shotgun sequence.
ACCESSION   DAOOVC010000258 DAOOVC010000000
VERSION     DAOOVC010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13353_length_3730_cov_4.179864"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1117..1608)
                     /locus_tag="PLM79_18030"
     CDS             complement(1117..1608)
                     /locus_tag="PLM79_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88260.1"
                     /translation="MRLEKRQPSGREKDEAALELLEKLREQLYSTNISTVRQSAFHLS
                     WMQEDGLDILREALLNDTPRRTKSAAAYGLRKMRGRMRKHAEATLIEGTTSPDRLTAE
                     ISRNALVVIKKSKTATKQPPPQVGKHPGKFEIRDIPAKKRSRKQSQRRKRPDDPQSSR
                     RRQ"
     gene            2406..>3730
                     /locus_tag="PLM79_18035"
     CDS             2406..>3730
                     /locus_tag="PLM79_18035"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOV88261.1"
                     /translation="MQIEPHGSTVRRKKLGELLCEKAYLDEVNLGVALAEQKVKPRQL
                     GQILLELGYVTQAQLNEALAIQAGIERVDLSDLSIASEVIGMVPAELVSKHNILPLWR
                     QNGRLAVAMTDPLQIQVVEDLRLVTGCMIQRFYADPTQLEHAVLRFYGSNVARMLDNL
                     APAEQPMDAEDSGNGDFSPAKLSELAREPSLVNLVNLIIYEAIESRASDIHIEPFDRE
                     VKIKYRIDGLLVEKSPSPKRLHAAIVSRIKIMANMNIAERFVPQDGHIEFMGKKGKID
                     IRVSTVPTVFGEDVALRLLDRSASLISLSDLGMNEATLEGFCKCLKKTHGIVLVTGPT
                     GSGKTTTLYAALNQIYSPTIKMITIEDPVEYQLEGINQIPVNPKRGLTFATGLRHILR
                     HDPDVVMVGEIRDKETAEIAIRAALTGHLVFSTLHTNEAAGAVKGRKHQ"
BASE COUNT          817 a          999 c         1058 g          856 t
ORIGIN      
        1 cgatacgccc aggattgccg ccaaagcaat gaatagactc ctccgtaaca taaccactcc
       61 tttccgatac cgtctccgga ccaaccgaag ccggatttgt ctggccgatg aaggccatat
      121 ccctgatgtt ttttcggggt ctcacgcgag cacaaccgct ctcatgcagg gacccacgat
      181 cgtgcaactc gctaaaatcg tttccgatac tctatctatt ggacttcgag accattttgg
      241 cgcagccctg gccacttttt cgttgcggaa ccggacaacg aacttggcag ccggggccgg
      301 gaggccttgt ttccccggcc ccggaaccag ggagtcgatc ggtcttcctt gaccggtcga
      361 ctcacaacaa tgcgaacgca tccgagtgca gaagatgtgg gctgctttgt gggaatgaaa
      421 atcgcaggtc gcagacacca cgtcaacaga cccggcattt ccggaacggc ttccatgtcg
      481 ttctctgggg gtagcgttca ccggttttcg cgcggtcatc cggcaagatg cgtcgggaac
      541 gtggtttatg ggcggttccg actcgctgga cgtggggctt tgagaaaaga aaactcccag
      601 ccatccaaca cgatttgaga aaacagggaa agggaaaagg aggtctctga ggaaaaatga
      661 aacaatgcac tccggaagaa ctgtccaacg acagcccaca ggcgcagtgc aaaaaaacag
      721 acactcaccc tctgaagtgc ccactccaag cctccttgct acgattcgga ttacccgttc
      781 tccgggaaca ccgttacttg acaaggcatt ttgccctgcc atcgtccttg ggaagcacac
      841 ccaaccttgg tggctccgat ggctccaggc tggccctcct ccattgaggg accgccatta
      901 cgccaccctt gtagatttgt ggcccggtaa tgtcaatgaa aaaatgtgaa taacaacgca
      961 ttttttctcg gacgatgatt ccaagttctt gcgttccagt tatttatagt gccggatgaa
     1021 catgcgagca cggaaaagcc ttggtttttg gtttcaccgg atttcttgcg gggccaaccc
     1081 gatccgcgac ggtcacgcct gctgtaaggg aaagattcac tgtctgcgac gcgaggactg
     1141 aggatcgtcg ggtcgctttc ttcgctgaga ctgctttctg gaacgcttct ttgcaggaat
     1201 gtcgcggatc tcaaatttgc cgggatgctt gccgacttgg ggcggcggtt gttttgtggc
     1261 tgtcttgctt ttcttgatca cgaccagggc atttcgggaa atctcagcgg tcaggcgatc
     1321 cggactggtg gttccttcga tcaaggtcgc ctccgcgtgt tttctcattc gcccccgcat
     1381 ctttcgaagt ccgtaggctg cggcgctctt ggttctacgg ggggtgtcgt tcaggagggc
     1441 ttcccggagg atgtccaagc catcctcctg catccaggac aagtgaaatg cgctctgtcg
     1501 tacggtcgaa atattggtgg aatacagttg ttcacgcaat ttttccaaca actcgagggc
     1561 tgcttcgtct ttttcgcgac ccgagggttg tcttttctca agtctcatag caccacctcc
     1621 tgtgtgcctc gacctctcca atccactgtg gcaagccgct tgacctccat ttagcgggaa
     1681 acaagggcac cgatgctgct ggaaacactg gaaaaaccaa ggtacaggga gatgccaaaa
     1741 ccccttttga catacgataa gcgcatggct tttctctccc cactctatgc ctggacttga
     1801 gcatccttgt ttgtcctcca gtcagaaagc acgtgtcgtt gaggtcgccg cgtcaactcg
     1861 cgtgccgatg gagtgtgttc cccaccggct gtcttcaagc cccaaagcgg tgcgtacgct
     1921 taatttgtcg cgcgacgcca aattgtaggc atattattgt cccagcagca cttgagagtg
     1981 tcaaatgatt tttttccggc tccgacaagg gattgttctg atctgtctgg gagtgaacct
     2041 tggcctccgg gtgccgttgc ccaaagagaa gggggatcga ttggtaattg ccgccgagga
     2101 gccaaggtat acggcagaat tgtatttttg tgtaacaaag ctgcacctat aagcgtctat
     2161 ccctaatagt agcaatgggg aggagttgtg tggtctgagg ccgcggtgcc tggtagaatg
     2221 ggcgcgacgc ggtggacgcg gctgtcggaa tcccttcagc cggacgtcaa gccctgttcg
     2281 cacgacagag cggttttggc gaagcgggcg agaggtttcg tgggcggcga aagtgtatag
     2341 atagaatgta aactactgcc ctgtatgaag ttgctggcgg tgggaattgg aattgagggt
     2401 aaggaatgca aatagagccg cacggcagta cagttcgaag aaaaaagctc ggcgaactgc
     2461 tttgtgaaaa ggcatacctg gatgaggtca acctcggggt ggctctggcc gagcagaagg
     2521 tcaagccccg ccagttgggc cagatccttc tggagttggg gtatgtgacg caagctcagc
     2581 tcaatgaagc acttgcgatc caagccggaa tcgagcgagt ggatttgtcc gacctctcca
     2641 tcgcaagtga ggtgatcggc atggttccgg cggagctggt gagcaaacac aacatcttgc
     2701 cgctgtggcg tcagaacggt cgtttggcgg tggcgatgac cgatccgcta cagattcagg
     2761 tcgtggagga tctgcgtctg gtcacgggtt gcatgatcca gcgattttat gccgacccca
     2821 ctcaattgga gcatgcggtt ctgcggtttt acggcagtaa tgtggcgcgg atgctggata
     2881 acctggcccc cgctgagcaa ccgatggatg ccgaagactc cggcaatgga gatttctccc
     2941 cggctaagct gagcgagctg gcgcgcgagc cgtccttggt caatctggtg aatctgatca
     3001 tttatgaggc catcgagtcc cgagccagcg acattcatat cgagccgttc gatcgcgagg
     3061 tcaagatcaa atatcgcatc gacggtctgc tggtggaaaa gtccccctct ccgaagaggt
     3121 tgcacgccgc gatcgtatcc cgcatcaaaa tcatggccaa catgaatatc gccgagcggt
     3181 tcgtccccca ggacggccac atcgagttca tgggcaagaa gggtaagatc gacattcgcg
     3241 tatcgactgt cccgacggtt ttcggagaag acgttgccct gcggcttctg gatcggtctg
     3301 cgtcgctgat cagtctgagc gatctcggaa tgaacgaagc gacgctggag ggcttctgta
     3361 agtgcctgaa gaagactcat gggatcgttc tggtcaccgg acccaccggg tccggcaaga
     3421 caacgactct ctatgccgct ctcaaccaga tttattcgcc tacgatcaag atgatcacga
     3481 tcgaggaccc ggttgaatac cagctcgagg gcatcaacca gatcccggta aatcccaaac
     3541 ggggattgac tttcgccacg ggattgcgac acattctccg tcatgatccg gatgtcgtca
     3601 tggtcggcga aatccgcgac aaggagaccg cggaaatcgc gatccgggcc gctctcacgg
     3661 ggcatctggt gttttcgacc cttcatacca acgaggccgc cggtgctgtg aagggtcgaa
     3721 aacaccagat
//
LOCUS       DAOOVC010000259         3656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13693_length_3656_cov_5.277701, whole genome shotgun sequence.
ACCESSION   DAOOVC010000259 DAOOVC010000000
VERSION     DAOOVC010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13693_length_3656_cov_5.277701"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="PLM79_18040"
     CDS             complement(<1..811)
                     /locus_tag="PLM79_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88262.1"
                     /translation="MLPGHLRSNLAALAARSPDLFERLCEPVESDHVDLKSAASGSPT
                     AVLKQSRPVFLHSAEDPLREAREAVRSLPEECRRGILCLGLGLGYYLNAFLRDADLSR
                     PILAYERDPWLLRLTLTCFDFSRDILAGRVRFLLGTDLGLLPGQELEDRFCWHHPVLG
                     SLYEEERRFVESGRVRPARKALVGAGGLFVSDLADALRQEGYAVLAWDPQRVSEKETL
                     RQIRTLDPHLVVTVNLCRGLPEICESLGIPLIVWEIDPSIERPLPGKSDLTR"
     gene            complement(841..2022)
                     /locus_tag="PLM79_18045"
     CDS             complement(841..2022)
                     /locus_tag="PLM79_18045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOV88263.1"
                     /translation="MPTFNNDTRFRGCAPDSTQQAASVKWNAEGEVLFSGGNTEEAKR
                     CFEKAVEFDPGSTKALNNLSVAHWTLGDHESSLCFLTRALETDPNDRDVILNCSKVFE
                     ELGKRRDALDVLRAYLQRKPWDDEVRREMLRLEESLAKAPSPGPADLFNEEGEKRFEQ
                     GKIEHARACFEIAAELDPRHARARNNLGVLHWREGDLQGALERFYSALELDSEDSDIL
                     FNSTRALCAAGHPETAGELLKVYLQKNPGDERAWADYAAMLRDGNNRHWDPRDLSPDL
                     SEVYLSMGKKLALSKDWIGASEAFSRSLSLNPGKADAYYALGCIHRELDQSDQALEMF
                     AEALALDAFHKPSVLASGEIFLSLGKPEEASRMYESFLKERKDRDVQQALKKIGDAGA
                     P"
     gene            complement(2149..>3656)
                     /locus_tag="PLM79_18050"
     CDS             complement(2149..>3656)
                     /locus_tag="PLM79_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV88264.1"
                     /translation="APLVLLELMAMRLPILGASIGGIPDFVKEGVNGFLYSHDSVESL
                     CRCIEYCIQNRPIIRKMRSSLRRPHSFQGYVNHLETVYRDLSSRKIGDASRYDLFVRL
                     DPGTSRPAIRWEGSQFVIHSLALVNRELCLQLMDKGCEVSIVPFEPDQFGPEKDPRFS
                     RIAERVHKPLSREPDLHVRHQWPPNFVPPPQGHWVMIQPWEYGRLPEDWIEPMSNLVD
                     EIWVPSRYVLKTYLASGIPADRVRVIPNGVNTRLFHPQARPISLATRKKYKFLFLGGT
                     IWRKGIDLLLQAYGEAFRREDDVVLVVKDMGQNSFYRGQGAGKLIGELQGNPKFPEIL
                     YLTEDLDEQQMPGLLTACDCLVHPYRGEGFGLPVLEAMACGLPVIVTAGGATDDFCTP
                     ETAYFIPSRPKGYFPNDMKLAGGALWVLEADVNALKNELRHVYEHPEEARRKGQRAIE
                     KVASQYTWDKVAEQVTKRAEALRNRPIRRMGTIPGPVEPCAASPAFAHNTV"
BASE COUNT          699 a         1116 c         1125 g          716 t
ORIGIN      
        1 agcgggtaag gtccgacttt cccggcaggg gtcgttcgat gctggggtcg atctcccaca
       61 cgatgagggg aatgcccagg gattcgcata tctccggaag accccggcaa aggttcaccg
      121 tgaccacgag gtgcggatcc agggtccgaa tctgacggag ggtttccttc tcggaaaccc
      181 gctgcgggtc ccacgccagg accgcgtaac cttcctgccg aagggcatcg gcaaggtccg
      241 atacaaagag tcctcccgct ccgaccagcg ccttccgggc cgggcgcacc cggccggatt
      301 ccacaaaacg cctctcttcc tcgtaaaggg aaccgagaac gggatgatgc cagcagaagc
      361 gatcttcaag ttcttgtccc ggcaaaaggc ccaaatccgt ccccaggaga aaccgcaccc
      421 ggcctgccag gatgtcccgg gaaaaatcga agcaggtgag ggtgagccga agcagccagg
      481 ggtcccgttc ataggccagg atgggtctcg agagatcggc gtcccggagg aaggcgttca
      541 ggtagtagcc caggcccaag cccaggcaca gaattccccg ccgacactcc tcgggaagag
      601 agcgcacggc ttcccgggcc tctcgaaggg gatcttcggc cgaatggagg aaaaccggcc
      661 gggactgctt caaaacggcg gtgggactgc ccgaggccgc cgacttgagg tccacatggt
      721 cgctctccac gggttcgcac agccgttcga acaaatccgg ggatcgagcg gccagggcgg
      781 ccaggttcga gcgcagatgg cctggaagca tggtcgagtc ctcttggggt aaggctcgat
      841 tcatggcgcc ccggcatcgc cgattttctt gagggcctgc tggacgtccc ggtctttgcg
      901 ttccttgaga aacgattcgt acattctcga ggcttcctcg ggctttccca gggagaggaa
      961 aatttctccc gaagccagga ccgagggttt atggaaagcg tccagtgcca gggcttcggc
     1021 aaacatctcc agggcctggt ccgactgatc gagttcccgg tggatgcaac ccagggcgta
     1081 gtaggcatcg gccttgcccg gattcaggga caaggagcgg gagaacgctt cgctcgctcc
     1141 gatccagtct ttggacaggg ccagtttctt gcccatggaa aggtagacct ccgagaggtc
     1201 cggggacagg tcccgtggat cccagtgacg gttgtttccg tcccggagca tggcggcgta
     1261 gtcggcccac gcacgttcgt ccccggggtt tttctggagg tagactttga gcagctcgcc
     1321 ggcggtttcc gggtgtccgg cggcacagag ggctctagtg ctgttgaaca gaatgtccga
     1381 atcttccgaa tccagttcga gggcgctgta gaaacgctcg agagctccct gcaaatctcc
     1441 ttcccgccag tgcagcaccc ccaggttgtt ccgggcgcgg gcatgacggg gatccagttc
     1501 cgcggcgatc tcgaagcatg cccgggcatg ctcgatcttt ccctgctcga accgcttttc
     1561 cccttcctcg ttgaagagat ccgccggacc gggcgaaggg gccttggcaa gggactcctc
     1621 gagccgcagc atctcccgcc tcacctcgtc atcccacggc ttccgttgga gataggcccg
     1681 gagcacatcg agggcgtccc gcctctttcc cagctcctcg aagaccttac tgcagtttag
     1741 gatgacgtcc cggtcgttgg ggtccgtctc gagggcccgg gtcaagaaac aaagggagga
     1801 ttcgtggtct cccagggtcc agtgggctac gctcaggttg ttgagcgcct tggtactgcc
     1861 ggggtcgaat tccacggctt tctcgaaaca gcgcttggcc tcttcggtgt ttcccccgga
     1921 gaagagcacc tccccctcgg cgttccattt cacggacgcc gcctgttggg tgctgtcggg
     1981 ggcgcatccc cggaaccgtg tgtcgttgtt aaaggtcggc atatcgaact ccagtcgttg
     2041 ggttgttcaa agtggtgctt cctgggaggc accgggacct tggactcgaa tccatcctcc
     2101 caagtcaaat attgttccat cccgggcgaa aaagtcgccg cttcccgctc aaaccgtgtt
     2161 gtgtgcaaac gccggagagg ccgcgcaggg ttcaacaggc ccggggatcg tccccattct
     2221 ccgtatggga cgattcctca aagcctcggc tctcttggtg acctgttcgg caaccttgtc
     2281 ccacgtgtac tgggacgcca ctttctcgat agcccgctgc ccctttcgcc tcgcttcctc
     2341 gggatgttca tagacgtggc gcagctcgtt cttcagggcg tttacgtcgg cttccaacac
     2401 ccaaagcgcc cctccggcca gcttcatgtc gttggggaaa tatcctttgg gccgggaggg
     2461 aatgaaatag gccgtctcgg gggtgcagaa atcatccgtc gcgccgcccg ccgtgacgat
     2521 cacgggcagg ccgcacgcca tggcttccag gaccggcaaa ccaaaccctt ctccccggta
     2581 cggatgaacc aggcaatcgc aggcggtaag aagccccggc atttgctgtt cgtcgaggtc
     2641 ctcggtcagg tagaggattt ccggaaactt cgggtttccc tgcaattctc cgataagttt
     2701 tcctgcccct tgccctcgat agaagctgtt ctgccccatg tccttgacca ccaggaccac
     2761 gtcgtcctcg cgtcgaaagg cctccccata ggcctgcaac agcaaatcga tccccttgcg
     2821 ccagatggtc ccgcccagga acaggaactt gtacttcttg cgggtggcca gagaaatggg
     2881 ccgagcctgg gggtggaaca gccgcgtgtt cactccgttg ggaatcaccc gcacgcgatc
     2941 ggccggaatt ccgctggcca ggtaggtttt caggacgtac ctgctgggga cccagatttc
     3001 atccaccaga ttcgacatgg gttcgatcca gtcttcgggc aaccttccgt actcccaggg
     3061 ctggatcatg acccagtgac cttggggggg cggaacgaaa ttgggaggcc attggtgtcg
     3121 cacatggagg tcgggctccc tcgagagggg cttgtgcacc ctttccgcaa tccgggaaaa
     3181 gcgcgggtcc ttctcagggc cgaactggtc gggctcgaag ggaacgatgg acacctcaca
     3241 ccccttgtcc atgagctgca ggcacagctc ccggtttacc agggccaggg aatgtatgac
     3301 gaactgggaa ccctcccagc gaatggcggg tcgggaagtg cctggatcca gccggacgaa
     3361 aagatcatac cgggaggcat ccccaatctt gcggctcgac aggtcgcggt aaacggtttc
     3421 caggtgattc acgtagccct ggaaggagtg gggccggcgc agggaagaac gcatctttcg
     3481 gatgatcgga cggttttgga tgcagtactc gatgcatcgg cacagggatt ccacggaatc
     3541 gtgggagtag agaaacccgt tcactccttc cttcacgaaa tcgggaatcc ccccgatgga
     3601 agcccccagg atgggcagac gcatggccat gagctcgagc aggaccaaag gcgcac
//
LOCUS       DAOOVC010000260         3590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_13995_length_3590_cov_5.100424, whole genome shotgun sequence.
ACCESSION   DAOOVC010000260 DAOOVC010000000
VERSION     DAOOVC010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13995_length_3590_cov_5.100424"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PLM79_18055"
     CDS             complement(<1..996)
                     /locus_tag="PLM79_18055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV88265.1"
                     /translation="MKKIRVTVLVSSLHIGGAEQLLLDLLRNISTQKLDLLVCFLREP
                     GMLGREVLTLGHRCRTHLLRSRWDPLGVLKTARLFREEKTDLLFLINHRNALFHGVLG
                     AKIAGVPAIVNWENETFKTYSFNFLTVLNHRLLHLGVDEVVAAAMGHADYLVAHEKVP
                     RRKVTTIYNCVDPAKFRSNLSRAEARIRLGIPVDSPVVSVIGVLRPDKNHRMFLEAAR
                     RVLDEIPAARFLIIGDGPERKGLEEEAGRLEIAGAVHFLGFRRDLGDLLAAVDVNALS
                     SKPEQETLSVAVVEAMSAGIPIVSTDVGFMNEVVIPEKTGYLVPVGDASSLASGIL"
     gene            complement(1013..2293)
                     /locus_tag="PLM79_18060"
     CDS             complement(1013..2293)
                     /locus_tag="PLM79_18060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV88266.1"
                     /translation="MKILLCNKFFFLNGGVERYLRDLLEYLPRAGHTPVPFSVRYRGS
                     WESPYSRYFLPPPTRSGKPHFKDVRLVHSNLLRLADRGVYSFEARCKLSRLIEEVPDI
                     RVAFLLNTCNYMSLSILHTLRRHRVPSVMFLGDYHLVCPNYLLLRDGAPCTLCIHGDY
                     LHGVRHRCVKGSRLASAVRATSMYVQKWLRMYDLVQAFVVPCRFMGEKLVDGGFPRDR
                     IHLIPYPVTETGVAFESRVKKDYILYFGRITYEKGLDTLILAFQQSNPPVDLYLLGRD
                     YDGEKERLRGLIRPEFAQRIRFLGFKEGAELSRWIGEALCTVVPSRWYDNAPLSVYES
                     FLHRTPVLAADIGGIPEQVEVGVTGRLFPPDSVPGLAEALTSMLSDRERLVEMGRNAR
                     EHVLRDCTMEKHGEKLLGLFETVVRSHPTGGFRA"
     gene            complement(2338..>3590)
                     /locus_tag="PLM79_18065"
     CDS             complement(2338..>3590)
                     /locus_tag="PLM79_18065"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88267.1"
                     /translation="AVHPTDPKVLLAATHPEQWPHMLFESRDGGRSWKLLQEFNLSPT
                     APSNWARTLNGPEKIVFSPGGDRVYLADWWNLWERSGEEGPWTQLHHGLQNSVVNDIR
                     VDPRDPRRWLVCAADSGVMVSQDAGKTWTRKMNGVADGHAQALDFSRRDPRKMFLVTN
                     PWSKKGRIHVYRTADGGETWQDVGFPMPSNPLASLGFADGSASGVAADPVRDEVAYVT
                     TNGYGIFKTVNGGKTWDAVNEGLATPYVKGPRSLLIHPKTPDVLWVSTQKGGVYKSVN
                     GAKSWRLVSPQGPFCFGMAVDPSNPSRVLAACAEKKVLLSEDEGNSWTEILLPGERPS
                     HVAAYSLAFHPESPGCVFAGTLAYDLKAAHGLFHSRDGGRSFRPCALPLPPVNLNVLE
                     AIPGLGCPVLLGFNGIGIYQGRGD"
BASE COUNT          716 a         1104 c         1108 g          662 t
ORIGIN      
        1 aagaatcccg gatgccaggg aagaagcgtc gcccaccggt accaggtacc cggtcttttc
       61 cgggatcacc acctcgttca tgaagcccac gtccgtggag acgatgggaa tgcccgcgga
      121 catggcttcc accaccgcca ccgaaagggt ttcctgctcg ggcttggaag agagggcgtt
      181 gacgtccacg gcggccagca ggtcccccag gtcccgccga aatcccagga aatgaacggc
      241 gcccgcgatt tccagcctcc cggcttcctc ctcgaggccc tttctttccg ggccgtcccc
      301 aatgatcagg aaccgggccg cgggaatctc atcgaggacc cttcgggcgg cttccaggaa
      361 catgcggtgg ttcttgtcgg gacgcaacac cccgatgacg ctgaccaccg gcgagtcgac
      421 gggtatcccc agccgtattc ttgcctcggc ccgggacagg ttcgaccgga acttggcggg
      481 atccacgcaa ttgtaaatgg tggtcacctt ccgacggggg accttctcgt gtgcaacgag
      541 gtaatccgcg tgtcccatgg ctgcggccac cacttcgtcc acccccaggt ggagcaggcg
      601 atgattgaga accgttagaa agttgaacga gtaggtcttg aacgtctcgt tttcccagtt
      661 aacgatggca gggacccccg cgatctttgc ccccaggact ccatgaaaca gggcgttgcg
      721 gtgattgatg agaaagagca ggtcggtctt ttcctctcgg aagagacggg ccgttttgag
      781 cacacccagg gggtcccacc gggaacgcag caggtgggtc cggcagcgat gacccagcgt
      841 gagcacctcc cggcccaaca tccccggttc ccgcaaaaag cagacgagaa gatccagctt
      901 ttgggtggag atattgcgca acagatcgag gagcagctgc tccgctcccc cgatgtgcag
      961 ggaagacacc agaacggtca cacggatctt cttcatcgcg gtttttcccg tctcatgcac
     1021 ggaatccccc cgtcgggtga gaacgaacga cggtttcgaa gagccccagg agcttctccc
     1081 cgtgtttttc catggtacag tcccggagga cgtgctcccg ggcgtttctc cccatttcaa
     1141 ccaggcgctc ccggtcggaa agcatggacg tcagcgcttc ggccagcccc gggacggaat
     1201 ccgggggaaa caacctgccc gtaaccccga cctcgacctg ttccgggatg ccgccgatgt
     1261 cggcggccag cacgggagtc cggtgaagga agctctcgta gacgctcagg ggagcgttgt
     1321 cgtaccagcg gctggggacc accgtacaca gggcctcccc gatccaccgg gaaagttccg
     1381 ccccttcctt gaaccccaaa aaacgaatcc gttgggcgaa ctcgggccgg atcaacccgc
     1441 gaaggcgttc cttctccccg tcgtagtccc tgcccaaaag atacagatcc acgggggggt
     1501 tggattgctg gaaggccaga atgagggtat ccagcccctt ttcgtaggta atgcgtccga
     1561 aatacaggat gtagtccttc ttgacccggg actcgaaggc aacgccggtc tcggtcaccg
     1621 ggtacggaat gagatggatg cgatccctcg gaaaaccgcc gtccaccagc ttctccccca
     1681 tgaatcggca cggcaccacg aaggcctgca ccaagtcgta catgcggagc catttctgaa
     1741 cgtacatgga agtcgctcga accgccgatg caagacggct ccccttgacg caccgatggc
     1801 gaaccccgtg aagatagtcg ccgtgtatgc aaagggtgca gggtgcgccg tcgcgcagga
     1861 gaaggtaatt gggacacacc aggtgatagt cccccaggaa catcactgag gggacccggt
     1921 gacgtcgcaa ggtgtggagg atgctcaagg acatgtaatt gcacgtgttc aagaggaacg
     1981 ccacccggat atccgggacc tcttcgatca atcgggagag cttgcatcgg gcttcgaagg
     2041 aataaacgcc ccgatccgcc aggcgcagaa gattgctgtg cacaagccgc acgtccttga
     2101 aatggggttt ccccgaacgg gtcgggggag gaagaaagta acggctgtag ggggattccc
     2161 agcttccccg gtaccggacg ctgaagggaa cgggagtgtg ccccgcccgg ggaagatact
     2221 cgagaaggtc cctaaggtat ctttccactc ctccgttgag gaaaaagaac ttgttgcaca
     2281 ggagaatctt catggccctc gtccatgggt tcgggggggg tgttcgagcc gcccggttca
     2341 gtcacctcgt ccctgataga tcccgattcc gttgaacccc agcagtactg ggcacccgag
     2401 gccgggaatg gcttccagca cattcaggtt gaccggagga aggggaaggg cgcaaggtcg
     2461 gaaggatcgt cccccgtccc ggctatgaaa caggccgtgg gcggccttga ggtcgtaggc
     2521 cagggtcccc gcaaagacgc acccgggaga ttcgggatga aaggccaggg agtaggccgc
     2581 cacgtgcgac ggtcgttccc ccggcaggag gatttccgtc cagctgtttc cctcatcttc
     2641 gctcaagaga actttcttct cggcgcaggc agccaggacc ctcgaggggt tcgacgggtc
     2701 cacggccatg ccgaaacaaa agggaccctg agggctgacg agacgccagc tctttgctcc
     2761 gttcacgctc ttgtacactc cgcccttttg ggtgctcacc cacagcacgt cgggggtctt
     2821 gggatggatg agaagggaac ggggtccctt cacgtaaggg gttgcaagac cttcgttgac
     2881 cgcatcccag gtcttaccgc cgttgacggt tttgaagatg ccgtacccgt tggtggtcac
     2941 gtacgccact tcgtcccgca ccgggtccgc cgccacgccg gaggcgctcc cgtccgcaaa
     3001 gcccagggag gcaaggggat tcgaaggcat cggaaagccg acgtcctgcc aggtttctcc
     3061 cccgtcggcg gtccggtaga catggattcg acccttcttg gaccacgggt ttgtgacgag
     3121 aaacatcttg cgggggtccc ggcgggagaa atccagagct tgagcatgac cgtccgcaac
     3181 cccgttcatt ttgcgggtcc aggtctttcc cgcatcttgg gataccatga ccccgctgtc
     3241 ggccgcgcag accagccagc gccgggggtc ccggggatcc acccggatgt cgttgacgac
     3301 gctgttttgc agcccgtggt gcagctgggt ccatggacct tcctccccgg acctttccca
     3361 caggttccac cagtccgcca ggtagacccg gtctccacca ggagaaaaca cgattttctc
     3421 gggtccgttg agggttcggg cccagttgga cggcgcagtg ggggaaagat tgaattcctg
     3481 caggagtttc cagctccgtc ccccgtcccg ggattcgaag agcatgtggg gccattgttc
     3541 cgggtgagtg gccgccagga gaaccttggg atccgtcggg tgcaccgcca
//
LOCUS       DAOOVC010000261         3578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14062_length_3578_cov_6.252058, whole genome shotgun sequence.
ACCESSION   DAOOVC010000261 DAOOVC010000000
VERSION     DAOOVC010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14062_length_3578_cov_6.252058"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1657)
                     /locus_tag="PLM79_18070"
     CDS             complement(179..1657)
                     /locus_tag="PLM79_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOV88268.1"
                     /translation="MAMEFIPLHCGEQLRVINPAGTIGIVTLWSRTDFVLGRLEAFGV
                     DLDPRSSSVAVLGTLYGNGLRELLRNLLYNPQLDTLLLVGRNRSGSAEELTAYFARGI
                     EPREDQSVEYQVPDGYEPPRSVRIAGTSRVLDDMVRPQDFQRPPTVFPVGDPQDPQSL
                     HRLKDFLARYCPAPGPIPPRIAVPLPVVRVSCFPSNPRGHCICEADPLDAWKELIHRL
                     CRFGHPVTLAKGGRRELQNVKVVVERPQERRRLLESLAENGHPPEAVHRYENDLLSPE
                     KHPDETYNYGHRMRAHFGLDSLAAVVRRLRDDPEDRKSFVVLWDPRMDLTGSRGHPCL
                     VSVFFRRFRDCLTLTGVFRTHNALDAWLFNFCGLAAVQRRVSEQVGMECGAITVFSHS
                     ITLDAGQMDRASRVAARRHFRIVEDPMGYFRITLDGDAILVEHRHGDLTLTTYRHKKA
                     SVLQYEISRDGAVSDLNHALYLGGQLARAQACLERGEEFIQE"
     gene            complement(1664..2269)
                     /locus_tag="PLM79_18075"
     CDS             complement(1664..2269)
                     /locus_tag="PLM79_18075"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HOV88269.1"
                     /translation="MNRIRPNWHEYFMLIAKIVSSRSTCNSRPTGAVIVKENHILSTG
                     YNGAMPGAPHCIDFPPLGNQPFCYRRARGIPDVDKYNFCKASHAEANAIAQAARYGIS
                     IEGATLYTTLAPCYVCLKLIATARIQGIYFEHSYESGTPERDAFWQTAVKEAGIAVFE
                     QLLISPETYEYILSDIRNITSRRRTLPTDFLERTAVPRDLE"
     gene            complement(2262..>3578)
                     /locus_tag="PLM79_18080"
     CDS             complement(2262..>3578)
                     /locus_tag="PLM79_18080"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOV88270.1"
                     /translation="FGVAVDVGTTTIVAKLVDLKTGKIAAVKASHNSQARYGEDVISR
                     MMFACKRGGLDTLVDAVGTTINSLVDAMVARAKISHQDITCFVAAGNTTMTHLLLGLS
                     PCSIRVEPYVPTADHFPQLPAAEVGLRGHPRAVLHCLPCVSSYVGGDITAGVLACGMN
                     DSPHMSALIDVGTNGEIVVGNSEWLVCCSASAGPAFEGGGTKCGMRAARGAVEKVRIQ
                     GDRVTFQTVEGEKPVGICGSGLIDLIAELFAEEIIDRSGKFLDLDHPRVQVTDETAEF
                     VVADASLTGTGKPVVVTEDDIANLIKSKGAILAAMKVVLDSTGMTFQDLDNLFVAGGF
                     GAHLDIEKAVMIGLLPDIPRDRIHFIGNGSLAGARLALLSSHAFHKAEAIARQMTYFE
                     LSVRPEFMNEFVAALFLPHTQLDLFPSVRESPARRKARGQGDGPYE"
BASE COUNT          668 a         1059 c         1174 g          677 t
ORIGIN      
        1 accaggggat ccggttcctc ggcccggtgg gtcatgagaa gccgtcccac cgcaggggaa
       61 cattccggat ccaggcccag gcgcgagcaa agaagcttgt ggggggactg ttccgccccg
      121 gtgaccaggg tctggccgat atcgaaaaag atcaccttgg gtttgggttt caagggcgtt
      181 attcctggat gaattcctct ccgcgttcca ggcaggcctg ggcgcgggcc agctgtccgc
      241 ccaggtacag ggcgtggttg aggtcggaga cggcgccgtc ccgggatatc tcatattgaa
      301 gaacgcttgc cttcttgtgg cgataggtcg tcaaggtcag gtctccgtga cgatgttcca
      361 cgagaatcgc gtccccgtcc agggtgatcc ggaagtagcc catgggatcc tcgacaatcc
      421 ggaagtgcct tcgagcggca acccgggaag cccggtccat ttgtccggcg tctaaggtga
      481 tcgagtggct gaacacggtg atggcgccgc attccattcc cacctgttcg ctcacccttc
      541 tttggacggc cgcaaggccg caaaaattga aaagccaggc gtccagggca ttgtgagttc
      601 gaaagacccc ggtcagggtc aggcagtccc ggaatctgcg aaagaacacc gagaccaggc
      661 agggatggcc ccgggagccg gtaaggtcca tgcgcgggtc ccacaggacc acgaaactct
      721 tgcggtcctc ggggtcgtct cgcaaccgcc ggaccaccgc cgccagggaa tcgagcccga
      781 aatgggccct catgcgatgg ccgtagttgt aggtctcgtc gggatgcttt tcggggctca
      841 acaagtcgtt ttcataccga tgaacggcct cgggcggatg cccgttttcg gcaagggatt
      901 ccaggagacg tctccgttcc tggggccgct cgaccactac cttgacgttc tgaagctctc
      961 gtctgccgcc ctttgcgagg gtcaccggat gcccgaagcg gcagaggcga tggatgagtt
     1021 ccttccaagc atccaggggg tccgcttcgc aaatgcagtg tccccgggga ttgctcggga
     1081 agcaggacac gcgaacgacg ggcaatggga cggcgatcct cgggggaatg ggtcccgggg
     1141 ccggacaata ccgggccagg aaatccttca gcctgtggag gctttggggg tcctgggggt
     1201 ccccgacggg aaacaccgtg gggggacgct ggaagtcttg cggccgaacc atgtcgtcca
     1261 gcacccggga agtcccggcg atgcgtacgc tccggggcgg ctcatatccg tccggcacct
     1321 ggtactcgac ggattggtcc tcccggggct cgatgccgcg ggcaaaatag gccgtcagtt
     1381 cctctgccga gcccgagcgg tttctcccta cgaggaggag ggtgtccagc tgagggttgt
     1441 agagcaggtt tcgcagaagc tcgcggagcc cgttcccgta aagggttccc aagacggcca
     1501 cggaactgga tcggggatcg agatccaccc cgaaggcttc cagcctgccc aagacaaagt
     1561 ccgttcggga ccagagggtg acgatgccga tggtgcccgc aggattgatc accctcagct
     1621 gttcgccgca atgaagggga ataaattcca tcgccatggg gtctcactcc agatcccgag
     1681 ggacagcggt tcgctccagg aaatccgtgg gcagagtcct tctccgggac gtgatgttcc
     1741 ggatgtccga aaggatgtac tcgtaggttt cgggcgagat gagcagttgt tcgaagaccg
     1801 cgattcccgc ctccttcacg gcggtttgcc agaaggcatc ccgttccggg gtgccggatt
     1861 cgtaggaatg ctcgaagtag atgccctgga tccgcgccgt ggcgatgagc ttcaggcaga
     1921 cgtagcacgg agcaagggtg gtgtaaagcg tggctccctc gatggagatt ccgtatcggg
     1981 ccgcctgggc gatggcgttg gcctcggcgt gggaggcctt gcagaagttg tacttgtcca
     2041 cgtccgggat tccccgggcc ctccggtaac agaacggctg gtttcccagg ggaggaaaat
     2101 cgatgcagtg gggagccccg ggcatggccc cgttgtaacc ggtggacagg atgtggtttt
     2161 ctttgacaat gaccgctccc gtcggccggg agttgcaggt ggaccgggag ctcacgatct
     2221 tggcgatgag catgaagtac tcgtgccagt tggggcggat tctattcata aggtccgtct
     2281 ccttggcctc gggcttttct ccgggccggc gattcccgga cggaagggaa caggtccaat
     2341 tgggtgtgag gaagaaaaag ggccgccacg aattcgttca tgaactccgg acgaaccgag
     2401 agctcgaaat aggtcatctg ccgcgcgatg gcttcggcct tgtgaaaagc atgggaggaa
     2461 agaagagcga gccgggcccc ggccagggaa ccgttgccga tgaaatggat ccggtcccgg
     2521 ggaatgtccg gcaacagtcc gatcatgacg gctttctcga tgtccaggtg agccccgaag
     2581 ccgccggcga caaacaggtt gtccaggtcc tggaaggtca tccccgtact gtccagaacc
     2641 actttcatgg cagcaaggat cgcccctttg ctcttgatga ggttggcaat atcgtcttcg
     2701 gtcacgacga cgggttttcc cgtgcccgtc aaggaggcgt ccgccaccac gaattccgcg
     2761 gtctcgtccg tcacctgtac ccggggatga tccaggtcca aaaacttgcc gctccggtcg
     2821 atgatctctt cggcaaagag ctccgcaatg aggtcgatca gcccggaacc gcagattccc
     2881 acgggctttt ctccctccac ggtctgaaag gtcacccggt ccccctggat ccggaccttt
     2941 tcgacggccc cccgggcggc ccgcatgccg cacttggtcc cgcccccctc gaaggccggg
     3001 cccgcggatg cggaacagca cacgagccat tcgctgttgc ccaccacgat ctcgccgttg
     3061 gttcccacgt caatgagggc gctcatgtgg ggggaatcgt tcatgccgca ggcaaggacg
     3121 cctgcggtga tgtcgccgcc cacgtaactg ctcacacagg gcaggcagtg gaggaccgcc
     3181 cggggatgtc cccgcaagcc gacttccgcg gcgggaagct gggggaaatg atccgcggtg
     3241 ggcacatagg gctcgactcg gatggaacag gggctaagcc ccaggagcag gtgggtcatg
     3301 gtcgtgtttc ccgccgccac aaagcaggtg atgtcctggt gggagatctt tgcccgggcg
     3361 accatggcgt ccaccaggga attgatggtg gtgcccacgg cgtccaccag ggtatccagt
     3421 ccgccacgtt tgcaggcaaa catcatgcgg gagatgacgt cctctccgta gcgggcctgg
     3481 ctgttgtgac tggccttcac ggccgcgatc tttccggtct tgaggtccac cagcttggcc
     3541 acgatggtgg tggtgcccac atccacggcc accccgaa
//
LOCUS       DAOOVC010000262         3575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14089_length_3575_cov_3.954545, whole genome shotgun sequence.
ACCESSION   DAOOVC010000262 DAOOVC010000000
VERSION     DAOOVC010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14089_length_3575_cov_3.954545"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="PLM79_18085"
     CDS             complement(<1..1238)
                     /locus_tag="PLM79_18085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOV88271.1"
                     /translation="MKSVLTCWIGKTDLRAPKEFEEVGLGPIAQAVEARSYEEVVLIS
                     DYPEAEVSPYLAWLRSRASTKVSIHYQKLSSPTNFGEIYKVAVRIVEEIQKAYSEEVS
                     LTFHLSPGTPAMAAVWIILAKTRFPAELIESSKPYGVRTASVPFDISAEFIPDLLRKP
                     DTELKRLSAGVPPEAPEFSDIVHRSEVMQRVITKARRVSPRSVPVVIEGESGTGKELL
                     ARAIHNASPRFGKPFVAVNCGAIPLELIESELFGHQKGAFTGADRTRTGHFETANGGT
                     LFLDEIGELPLSAQVKILRTLQEGEIMRVGTSTPIKIDVRVIAATNRSLIEEVAQQRF
                     RADLFYRLAVVILYLPPLRERSGDLTLLIDRLLDQINLESSTEPGYVHKKISAAAKNL
                     LLLHSWPGNVRELLNTLRRAA"
     gene            1369..2646
                     /locus_tag="PLM79_18090"
     CDS             1369..2646
                     /locus_tag="PLM79_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88272.1"
                     /translation="MLPLCSQETRGERAAIAGPLANSSEEENMPSTVTFFPVDNGDMT
                     LIKLDDSDKTAILVDINIRQDCGKDDNGMCDVVRELRKQLRKDAEGRPYVDVFLLSHP
                     DEDHCRGLQDQFHLAPLDTYNFEPPEGKELKIVIREIWSSPMVFRRAGKIHTLCEDAK
                     AFNTEARRRVKHFRENKSAGAGDRILIIGEDENGKTDDLEDILVKTDEVFNRVNGKTN
                     NSISMRVLGPLPPQETEELEDELSKNHSSVILQFSIKGGDKADACLYLTGGDAEVLIW
                     EKLWERNKDRKSCLEYNLMLAPHHSSWHSLSYDRWNGGKGNPQVSKDAKSALSQARDG
                     AYIVSSSKPIKDDDSDPPCWGAKQEYLSILKGVKGEFFCTGEYPDEEEQEPLQFKVFK
                     EGPQPPAKKSAPVEGASVLSGSIRKPLTHGWKR"
     gene            2646..>3575
                     /locus_tag="PLM79_18095"
     CDS             2646..>3575
                     /locus_tag="PLM79_18095"
                     /inference="COORDINATES: protein motif:HMM:NF025813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88273.1"
                     /translation="MKKHKMRQSGGNGRAQQMHHEAERAARLIQAHKAVARLVSSPKS
                     IDGGGFSISFDMEVALPSGARAAGISETGVKAQERVVLEFPPAYPLKAPRIYLRHDFN
                     RSLPHFNPMPGFGEGDLVTPCVYEGSLDELMHQCGDGLSEILNHLSEWLGKAAVDDLI
                     DSNQGWEPVRRDSILGSIIFDAGKLRSTADAQPQGGACFLGCTYAQNHLNGRYWVSRV
                     SHYDPGPITPALLANTLAFRKRFEELEFMASAGIFAWPADVPGGGGSAECGKYLPEKV
                     SNLKELLERADDYGCGRYLRSKLSELTWACSPLN"
BASE COUNT          876 a          949 c          983 g          767 t
ORIGIN      
        1 gcagcccggc gcagcgtatt gagtagctct ctcacatttc cgggccagct gtgcaatagc
       61 agaaggttct ttgctgcagc ggagattttc ttatggacgt aacccggttc agtagagctt
      121 tccagattga tctgatcgag caggcgatcg atgaggagcg tgagatcacc ggagcgttcc
      181 cgaagagggg gaaggtagag gatgaccacc gccaagcggt agaaaaggtc tgctctgaaa
      241 cgttgttggg caacctcctc gatcagtgaa cggttcgttg cagcaataac cctcacatcg
      301 atctttatgg gagtcgatgt gccgacacgc atgatctcac cttcctgcag tgttcgcaag
      361 atcttaacct gagctgagag tgggagctct ccaatctcat caagaaaaag ggtccctcca
      421 ttggccgttt cgaagtgccc tgtgcgggtt ctatcggctc ctgtgaatgc tcccttctga
      481 tgcccgaaaa gctccgattc gataagctca aggggaatgg cgccacaatt gaccgcgacg
      541 aaaggctttc caaatcgtgg gcttgcattg tgaatcgccc gagcgagcaa ttccttaccg
      601 gttccggatt ccccttcgat aacgacgggc actgaacgtg gggaaacacg cctcgctttg
      661 gtaatgactc tctgcattac ttcgctgcga tgaactatat ctgagaactc aggagcctcc
      721 ggtggaacgc cggcgctcag gcgtttcagt tccgtatcgg gtttccgaag cagatcaggg
      781 atgaactcgg ccgaaatgtc gaaggggacc gaagctgtgc ggaccccgta aggctttgag
      841 gactctatta attcagcagg aaagcgcgtt ttggcgagaa taatccagac cgcggccatt
      901 gccggagtcc ccggactcag atgaaatgtc aatgagacct cttcagaata tgccttctgg
      961 atctcctcga cgatcctcac ggccacttta tagatttcac caaagttggt ggggcttgag
     1021 agcttctgat agtgtatgga aaccttcgtg gatgcacggg atcttagcca ggcaagatac
     1081 ggagaaacct ccgcctccgg atagtcgctt atgaggacca cctcctcata ggatcgagcc
     1141 tccacagcct gagcaatggg gccaagcccc acttcctcga attcctttgg agcccgaaga
     1201 tcggtttttc caatccagca tgtgaggacg cttttcatgc aatcagaata caagttttgt
     1261 tgcgtcagta caagattttt tctgccaaaa ctgttgattt accttcgatc atgccctatg
     1321 tttttcgcca aaccctgatg atacaagaca tttaatgact tggcatgcat gttgccctta
     1381 tgcagtcagg aaacgagagg ggaaagggct gcgattgccg gtccacttgc aaactcatct
     1441 gaggaggaaa atatgccatc aacagttact ttctttccgg tagacaacgg cgacatgaca
     1501 ctcatcaagc tggacgactc cgataagacc gccattctgg tggatatcaa catccggcag
     1561 gattgcggaa aagacgacaa cggcatgtgt gatgtcgtca gagagctgag gaagcaactc
     1621 cgaaaagacg cagaaggccg cccctacgtg gatgtcttcc tcctcagcca tccggatgaa
     1681 gaccattgtc ggggacttca agaccaattc cacctcgccc cgctcgatac ctacaacttc
     1741 gagcctcccg agggcaagga gctgaaaatc gtcataaggg aaatatggtc ctcacccatg
     1801 gtgttcagga gagccggcaa aatccatacc ctgtgtgagg atgccaaggc cttcaacact
     1861 gaggccaggc gaagggtgaa gcatttcagg gaaaacaagt cagcgggtgc tggagaccga
     1921 atcctcatta tcggtgagga cgaaaatggc aagactgacg atctcgaaga tattctggtc
     1981 aaaaccgatg aagtcttcaa tcgtgtgaac ggcaagacca acaacagtat ctccatgaga
     2041 gtactcgggc cgttgccgcc ccaggagacc gaagaactgg aagacgagct gtccaagaac
     2101 cattcgagcg tcatcctgca gttctccatc aagggtggcg ataaagcgga tgcttgcctg
     2161 tatctcacgg gaggcgacgc ggaggtcctc atctgggaga agctctggga acggaacaag
     2221 gacaggaaat cttgcctcga atacaacctc atgctggctc cgcatcatag ctcctggcat
     2281 tcgctctcat acgacaggtg gaacggcggc aaaggaaacc cccaagtttc caaagatgcc
     2341 aaatccgcgc tatcgcaggc tcgagatgga gcctacatcg tatccagcag caaaccgatc
     2401 aaagacgacg acagcgatcc tccatgctgg ggcgcaaaac aggaatacct ttccattctg
     2461 aaaggcgtga agggggaatt cttctgcact ggcgaatatc cggatgaaga ggagcaagaa
     2521 ccgctccaat tcaaggtatt caaggaaggt cctcaacctc ctgccaagaa gagtgcgcct
     2581 gttgaaggag caagtgttct ttctggcagt atccgcaagc ccctcactca tgggtggaag
     2641 cgataatgaa gaagcacaag atgagacaat ccggtggtaa cgggcgcgcg caacagatgc
     2701 atcacgaggc tgagcgtgcc gcccgcctga tccaggccca caaggccgtt gcgcgactgg
     2761 tctcctcccc caagtccatc gatgggggcg gcttcagcat cagcttcgac atggaagtgg
     2821 ctttgccgtc aggggcaaga gcggctggga tcagcgaaac gggagtgaaa gctcaagaga
     2881 gggtagtcct tgaattcccg cctgcatatc cgctgaaggc ccctcgaatt tacctccgcc
     2941 atgatttcaa cagatccctt cctcacttca accccatgcc cggtttcggg gaaggggatc
     3001 tcgtaacccc ttgtgtgtac gagggcagtc tcgatgagct gatgcatcag tgcggtgatg
     3061 gtctatcgga gatcctcaac catctttccg aatggctcgg gaaggcggca gtggacgatc
     3121 tcatcgattc gaatcagggc tgggaaccgg ttcgccgtga cagcattctc gggtcgatca
     3181 tctttgatgc cgggaagctg agaagtaccg ctgacgctca accccaaggc ggcgcctgct
     3241 ttttgggatg cacctacgcg cagaatcacc tcaacggccg gtactgggtc tctcgtgtga
     3301 gtcactacga tccgggcccc ataacgccag ccttgttggc taacactctg gcattcagga
     3361 aacgattcga ggagcttgaa ttcatggctt ctgcggggat attcgcctgg ccggccgatg
     3421 ttcccggcgg aggcggatct gccgagtgcg gcaagtatct ccccgagaag gtgagcaatt
     3481 tgaaagagct tctcgagcgc gccgatgatt acggatgcgg aaggtacctc cggagcaaat
     3541 tgagtgagct cacatgggcg tgctccccgt tgaac
//
LOCUS       DAOOVC010000263         3544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14254_length_3544_cov_6.692749, whole genome shotgun sequence.
ACCESSION   DAOOVC010000263 DAOOVC010000000
VERSION     DAOOVC010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14254_length_3544_cov_6.692749"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLM79_18100"
     CDS             complement(<1..551)
                     /locus_tag="PLM79_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88274.1"
                     /translation="MQKKMFVQKGLPTAKPSYVRWLLVAAVGLILLILVTPLILSRTK
                     SSREAARKPIPERGERGMLVREIPKAPPPFLSDLQQPAAPPKEPPMPVWPGSPPAAHE
                     SPDKAKAAPATQAPTIAPDVAPATTAMTAPPEPARTVPPEGASGDLSAARKPVPPKTA
                     SAVPSSQGPPPPPAPRPPKDDPPR"
     gene            complement(566..1756)
                     /locus_tag="PLM79_18105"
     CDS             complement(566..1756)
                     /locus_tag="PLM79_18105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOV88275.1"
                     /translation="MAVAAKMLQFIENSSWIRKMFEEGTRLKAIHGNENVFDFSLGNP
                     NVPPPDIVKEKLLEIVQREEKGKHGYMPNAGLLSTRTAVSAFLCREHCVSTASDHIIA
                     TCGAAGALNIIFKALLDPGDEVVVPAPFFVEYGFYADNHGGVLKTVPSREDFSLDLPA
                     MEEAIGPRAKVVLINSPNNPTGQVYSAEEIRELSRILEDKSRSLGRTLYLVSDEPYRK
                     IVYDAETVPCIFEHYPHSLIATSYSKDLSLPGERIGFVAVNPGAEAVEKLVEALTLAN
                     RILGFVNAPALMQRLVVELQGICVDIDLYKRKRDRLVEGLQSAGYELTVPKGAFYLFP
                     RSPLADDIRFVRLLQEENILVVPGTGFGGPGHFRIAYCVDDATIENSLPGFRRAMERA
                     RKMR"
     gene            2089..2988
                     /locus_tag="PLM79_18110"
     CDS             2089..2988
                     /locus_tag="PLM79_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOV88276.1"
                     /translation="MTSHSFLQSLQAGEVLVADGATGTTLLERGLPRGTPGEAWVLDR
                     PREILGVHRSFIEAGARIVLTCTFGASPVRLRGGPLEDRMAAINRRAVELAREAAADR
                     PVLVGGSIGPSGVLLKPLGRLAPEEASDSFSRQARVLAEAGADLLVIETQYDLAEAAL
                     AVQAARSVSSLPVVCTFSFDRGSHTMMGVSPARVGKELESLGADVVGINCGRSLEENL
                     TALGQLRAHCSLPVWFKPNAGLPEVDGEGKARYRTTPEAMAVLVPAWIEAGARVVGGC
                     CGTSPQHLEQIARAVGKARGAAS"
BASE COUNT          598 a         1023 c         1220 g          703 t
ORIGIN      
        1 cggggggggt cgtctttcgg ggggcggggg gcgggggggg ggggggggcc ctgggacgag
       61 gggacagcgg aagcggtctt tgggggaaca ggcttccggg ccgcagagag gtccccggaa
      121 gctccctccg ggggaacagt ccgggccggt tccgggggcg ccgtcatggc ggttgtggcc
      181 ggggcgacat ccggggcgat ggtcggagct tgggttgccg gagccgcttt tgccttgtcg
      241 gggctttcgt gggcggcagg gggggacccc ggccaaacgg gcatcggggg ctccttgggc
      301 ggagccgcgg gctgctgaag atcgctcaga aaaggaggcg gggccttggg gatttcccgc
      361 accagcatcc ctcgttctcc ccgttcggga atgggttttc tggccgcttc cctcgagctc
      421 ttggtacggg acaggatcaa gggcgtaacg aggatcagca gaatcagtcc cacggcagcg
      481 accagcaacc accgcacgta ggacggtttt gcagtcggca ggcccttttg aacgaacatc
      541 ttcttttgca tggagctttc ccccgttacc gcattttgcg ggcccgttcc atggccctcc
      601 ggaagcccgg cagcgaattc tcgatggttg cgtcgtctac gcagtaggca atgcggaaat
      661 gaccgggtcc cccaaacccg gttcccggaa ccacgaggat gttttcctcc tggagcaacc
      721 gcacgaaccg gatgtcgtcg gccagggggc tcctgggaaa gagatagaag gcccctttgg
      781 gcaccgtgag ttcgtacccg gcggactgaa gtccctcgac cagccggtcc cgctttcttt
      841 tgtagaggtc gatgtccacg caaatcccct gcagttcgac gaccaggcgc tgcatgaggg
      901 ccggtgcgtt gacaaacccg aggatgcggt tggccagggt gagggcttcc accaactttt
      961 ccacggcttc ggcgccggga ttgacggcca caaagccgat cctttctccg ggcagcgaca
     1021 ggtccttgga ataggaggtg gcgatgaggc tgtggggata gtgttcgaag atgcacggga
     1081 cggtctcggc gtcgtagacg atttttcggt agggctcgtc ggacaccagg tagagggtcc
     1141 tgcccaggga gcggctcttg tcctcgagaa ttcgactcag ttcccggatt tcttcggcgg
     1201 aatacacctg gcccgtggga ttgttggggg agttgatcaa cacgaccttg gcccgcggtc
     1261 cgatggcttc ctccatggcg gggaggtcca gggagaagtc ttcgcggctg ggaacggtct
     1321 tgagcacccc gccgtggttg tccgcataaa agccgtattc cacgaagaag ggggcgggga
     1381 ccaccacttc gtccccggga tccaggaggg ccttaaagat gatgttcagg gccccggcgg
     1441 ccccgcaggt ggcgatgatg tgatcggagg ccgtggacac gcagtgttcc cggcatagga
     1501 acgcggatac cgcggttctc gtgctgagaa ggccggcatt gggcatgtag ccgtgcttgc
     1561 ccttctcctc gcgctggacg atctcgagga gcttttcctt cacgatatcg gggggaggga
     1621 cgttgggatt gccgagactg aagtcgaata cgttttcatt cccgtggatg gccttcagtc
     1681 gggtgccctc ttcaaacatt ttgcggatcc acgaagagtt ctcgatgaac tggagcattt
     1741 ttgcggcaac cgccatggtg gtttcctcct ggtttgtcca ttgacccgga ttgtggggtg
     1801 tcccctggaa tatagaagag attcccgtgc ctgtgcaagg attaaacccg ggtgggggag
     1861 gccgatggaa aaggagaccg ggtttcgtgt gcccgcaggc ccccggccag gggccggtcc
     1921 gtgtggactc cggcgaactc ctgtgctaga ctcgatctcc acaacggcaa acgatgcgaa
     1981 gcgatccgtt gcctacggag ccccgctcat tttctcgacg gggtgtggaa tccttttttg
     2041 ccattcttga gtcatcccgg agtcaacttc cggagggaga cctggaccat gaccagccat
     2101 tcattcctgc agagccttca agccggtgaa gtcctcgttg ccgacggggc caccggcacg
     2161 accctcttgg aacgcggtct tccgcgcggg actccggggg aggcctgggt actggaccgg
     2221 cctcgggaaa tcctcggggt gcatcgcagc ttcatcgaag ccggtgcccg gatcgttctc
     2281 acctgcacct tcggggcatc tcccgtccgg ctgcggggcg gccctctcga ggatcgaatg
     2341 gccgcaatca accggcgggc ggtggagctg gcccgggaag ccgccgcaga tcgaccggtc
     2401 ctggtggggg gatccatagg acccagcggc gtgctcctca aacccctggg gcgattggcc
     2461 cccgaggagg cctcggattc cttctcccgg caggcccggg tcttggccga ggccggggcc
     2521 gacttgctgg tgatcgaaac ccagtacgat ctggcggaag cggccctggc ggtccaggcc
     2581 gcgcgttcgg tgagttccct gcccgttgtg tgcactttca gcttcgatcg cgggtctcac
     2641 accatgatgg gggtgagccc ggcccgggta gggaaggagc tcgagtctct gggagccgat
     2701 gtcgtcggga tcaactgtgg acgcagcctc gaggagaacc ttacggccct cggacagttg
     2761 cgggctcatt gctccctgcc cgtctggttc aagcccaacg ccggacttcc cgaggtggat
     2821 ggagaaggaa aggcccgata ccgaacgacc ccggaggcca tggccgtgct ggtccctgcc
     2881 tggatcgagg ccggggcccg ggtggtcggc gggtgctgcg ggacctcgcc ccagcatctc
     2941 gaacagattg cccgggctgt cgggaaagcc cggggggctg cctcctagat cgagcgacgg
     3001 gaccggttcc aatcccggtt cgtcctctct aagaaggcgg gagtttcctg caacggggcg
     3061 caacccggtg cagaaatgat tcccgcccga gcggggaccc gcaaagagcg tgcacgcctg
     3121 cgggatgccc gccgtcgcgg gcacgacggg ctgaggcttt gggaatcgct caaaccgggt
     3181 gtccgcaaat gggggtgccc gggggttttc cattttgggg ccgcgggacg gttttgcctt
     3241 gccaagtccc gcaatttgtg gaaaaaaccc tcttgctgca acaagttgta tgccgccgtc
     3301 cgcctccgca gctgccttcc gtccggttcg ggtcgtgtcg tgtccatccg gggccgtaag
     3361 ttggacgggg gtggggtctg tgtgttgaca aagaaccgtg cttcgggtta tgaatcgacg
     3421 accatatcct acggaattcc aaggatttaa tagaaatcac cagctcagcg gcgaccatac
     3481 gaaggatggg gtttttatgc aaaagggggt gaaatccctc ctttttattt ccattcagga
     3541 gtga
//
LOCUS       DAOOVC010000264         3522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14388_length_3522_cov_6.558119, whole genome shotgun sequence.
ACCESSION   DAOOVC010000264 DAOOVC010000000
VERSION     DAOOVC010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14388_length_3522_cov_6.558119"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PLM79_18115"
     CDS             <1..802
                     /locus_tag="PLM79_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOV88277.1"
                     /translation="DLLKLVTIKTPEGGRDRLADLVLTEDQREEIAKAGKALEYREYL
                     RHIGLYEIGKILFVGPPGTGKTTTARALSQWFGLPFIEVKLSMIISHYLGETSKNIDR
                     VFVLAKKLSPCILFIDEFDFVAKTRTSDDHGAIKRAVNTLLKAVDDISLVEDGVLLIA
                     ATNYPQLLDYAAWRRFDKVMSFTLPDLVMRRKILGKILQKVHADIDLDELARLTEGST
                     GSDLRLVIREAVLNALLEDRKQISREDILRALDEFNRRNTDYRQNTNS"
     gene            complement(799..2946)
                     /locus_tag="PLM79_18120"
     CDS             complement(799..2946)
                     /locus_tag="PLM79_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88278.1"
                     /translation="MMGRSFLLEAGAAILFFGSLVHSPELARHGSEAVEELRSRGYRT
                     PTVEEPLRVYPAGSSGALESTHAGGWRPGVVSLRESPLGGLGPEVYLRHEILHEAAHR
                     TCGGKLPVWAEEAAAMSFSGELSGRAPEELPRRAELDHLRKRAAAGAPLDRANRETLA
                     RLVSWYGWPDKPCAVSEEIRKILHPSRASREEDFSYVLLSVPSARVLDTRGDLAARHP
                     PGSLLKIPYAAALKTADDSSLGEDLARSDTAKLLQRKASLDFDSYRLLVSMVPESSLG
                     RKISEEERRAGGDPFLKQYLGERGPDGMFPLEANLRELALLLRASLLFQPSRFRGLSQ
                     NGFLQESTLYGEPESHKRVLRTLGALSKTGTVADVRGTPLVGHLMVAWPVEDPAYLAV
                     FRILGRNGAANLRPASPVLERWSRQYPGRRGKVRVRLLTRTPPSSWSVLEECPGFERL
                     LSSGGRVGVSLCGQARILSTARGSRSRRMVRGVFEYSPDGQALVLETDPDTYADGVLD
                     AEAQDLEGEGRKALRAVIAWNGIHGASRHPEFPSLCDTTHCMVFRGEPVEGSPRKKER
                     TDPKLLELLDEISREHGMSWFSFSRGGADRWKKRMSSRELGALLHEDLILGIRRERTR
                     NGEVAVHLVYGDGEEVVPCEVFRNRLKLLSCPDSIWEDVPAGIWEFEGIGEGHGRGLS
                     LEKARALGKSGADARAILEDAYRPDPRPGPGSS"
     gene            complement(3035..>3522)
                     /locus_tag="PLM79_18125"
     CDS             complement(3035..>3522)
                     /locus_tag="PLM79_18125"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88279.1"
                     /translation="SLTTSSPLRRGRERSRTLLRIRGGTTEALDLSRPLRKGELVVSE
                     VKVRRDKQDLKTVPSRFVVVEDGIPSLGRAVDEDAAYLADGGIRPPEESYWANIKETQ
                     RHPDKTVRVAQVLPTGELILYQVWQVAFSGRATVPPARAYDMYDESIQGNTEAQSVRA
                     E"
BASE COUNT          626 a         1170 c         1103 g          623 t
ORIGIN      
        1 ggaccttctc aagctggtga ccatcaagac gcccgaaggg gggagggacc ggctcgccga
       61 cctggtgctc accgaagacc agcgggagga gatcgccaag gcgggcaagg ccctcgaata
      121 tcgcgaatac ctgaggcata tcggcctgta cgaaattggg aaaatccttt ttgtgggacc
      181 cccggggacg ggaaagacca ccacggcccg ggccctttcc caatggttcg ggctcccgtt
      241 catcgaagtg aagctctcca tgatcatcag ccattacctc ggggagacct ccaagaacat
      301 cgaccgggtc tttgtcctgg ccaaaaagct cagtccctgc attctattca tcgatgagtt
      361 cgatttcgtg gccaagacca ggacctcgga cgaccacggc gccatcaagc gggcggtcaa
      421 tacgctcctc aaggccgtcg acgacattag cctcgtcgag gacggggtgc tgctcatcgc
      481 ggccaccaat tatccccagc tgctggatta tgcggcctgg cggcgcttcg acaaggtcat
      541 gagcttcacc ctgcccgatt tggtcatgag acgcaagatc ctcggtaaga tcctccagaa
      601 ggttcacgcc gacatcgatc tcgatgaatt ggcccggctt accgaaggat ccaccgggtc
      661 cgatctgcgc ctggtgatcc gggaggcggt gctcaacgcc ctgctcgagg accgcaagca
      721 gatctcccgg gaggacattc tccgggccct cgatgaattc aatcgccgga acaccgacta
      781 tcggcagaac accaactcct aggaggaacc cggcccgggc cggggatcgg gccggtatgc
      841 gtcctcgagg atcgcccggg cgtcggctcc ggacttgccc agggcccggg ccttttccag
      901 cgacagcccc cggccgtggc cttccccgat cccctcgaat tcccatatcc cggccgggac
      961 atcttcccaa atggagtcgg gacaggacag cagtttcaat cggttgcgga acacctcgca
     1021 agggacgacc tcttcgccgt cgccgtacac caggtgaacg gccacctctc cgttccgggt
     1081 cctctctcga cgaatcccca ggatgaggtc ctcgtggagc agtgcgccca gctcccggga
     1141 actcatccgc tttttccacc ggtccgcccc gcccctggaa aaggaaaacc aggacatccc
     1201 atgttctcgg gagatttcgt ccaggagctc gagcagcttc gggtcggtcc gttccttctt
     1261 ccttggggag ccttccacgg gctctccccg gaagaccatg caatgggtgg tgtcgcacag
     1321 ggacgggaat tcgggatggc ggctcgcgcc gtgaattccg ttccacgcga tgaccgcccg
     1381 cagggctttt cgtccttctc cctcgaggtc ttgggcctcg gcgtcgagca ccccgtcggc
     1441 ataggtgtcc gggtcggttt cgagaaccag ggcttggccg tcgggcgagt attcgaaaac
     1501 acctcggacc atgcgccggg atcggcttcc ccgggccgtc gaaaggattc gagcctgccc
     1561 gcaaaggctc accccgactc ttccccccga ggacagcagc ctttcgaacc cggggcactc
     1621 ctcgaggacg ctccacgagg acgggggagt tcgagtcagc agccgcaccc gcacctttcc
     1681 ccgccggccc gggtattgcc gggaccatct ttcgagcacc ggggatgccg ggcgaagatt
     1741 cgccgccccg tttcttccca ggattcgaaa cacggccagg taggccgggt cctcgacggg
     1801 ccaggccacc atgaggtgcc cgaccagggg agtgcctcgc acgtccgcca ccgtccccgt
     1861 cttggaaagg gcgcccaggg tccgcagaac cctcttgtgg gactcgggtt ccccatacag
     1921 ggtggattcc tgcagaaagc cgttttggga cagtccccgg aaacgggagg gctggaaaag
     1981 gagagaggcc cgcaggagga gggccagttc ccgaaggttg gcctcgaggg gaaacatccc
     2041 gtccgggccc cgttctccca ggtattgctt caggaacgga tcgcccccgg ccctgcgctc
     2101 ttcctccgaa atcttccgcc ccagggacga ttccggtacc atggaaacga ggaggcggta
     2161 ggaatcgaag tccagggacg cctttcgctg gaggagcttt gcggtgtcgc ttcgggccaa
     2221 gtcttctccc agggaggaat cgtcggcggt cttgagggcc gccgcatagg ggatcttcaa
     2281 aagggaaccc ggagggtggc gcgccgcaag atctcccctg gtatccaaca cccgggccga
     2341 gggcacgctg aggagaacgt agctgaagtc ctcttcccgg gaagcccggg agggatgaag
     2401 gatcttgcgg atctcctcgg aaacggcgca gggcttgtcg ggccatccgt accaggagac
     2461 cagccgggcc agggtctcac ggtttgcccg gtccaggggg gccccggcgg ccgctctttt
     2521 tctcaggtgg tccagttctg cccggcgggg cagttcctcc ggcgcccggc ccgaaagctc
     2581 cccgctgaag ctcatggcgg cggcctcctc ggcccaaacc gggagtttgc ccccacaggt
     2641 tcgatgggcg gcctcgtgca ggatctcgtg ccgaagatag acctcggggc ccaggcctcc
     2701 gagagggctt tcccgaaggg acacgacgcc gggtcgccag ccgcccgcat gagtcgactc
     2761 gagggcgccc gaagatcctg cgggatagac cctcaggggc tcttccaccg tgggggttcg
     2821 atacccccta gatcggagct cctcgacggc ctcggaacca tgtcgggcca attccggcga
     2881 atgaaccagg gaaccgaaaa aaagaatggc cgctcccgct tccaggagaa aggacctgcc
     2941 catcatggac gccaacgtct tcagggtgcc gtctctttca tagatccccc gggggccgac
     3001 accgagcccg cccgggatgc ccggcggttc ccgcctattc cgctcggacg ctttgggctt
     3061 ccgtgtttcc ctggatgctt tcgtcgtaca tgtcgtaggc ccgggccgga ggaaccgtcg
     3121 cccggccgga gaaggccact tgccagacct ggtagaggat gagctctccc gtgggcagca
     3181 cctgggctac tcgaacggtc ttgtccgggt ggcgctgggt ttccttgatg tttgcccagt
     3241 agctctcctc cgggggacgg attcccccgt cggccaggta ggcggcatcc tcgtcgacgg
     3301 cccggcccag ggaaggaatc ccgtcctcga ccaccacaaa ccggctgggg accgtcttca
     3361 aatcctgctt gtccctgcgc accttcactt cgctcaccac cagctccccc ttgcgcagag
     3421 gtcgagagag gtccagggcc tcggtggtcc cgccccggat gcgaagcagg gtcctggacc
     3481 tctctcgacc tctgcgcaag ggggagctgg tggtgagcga ag
//
LOCUS       DAOOVC010000265         3485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14571_length_3485_cov_5.219242, whole genome shotgun sequence.
ACCESSION   DAOOVC010000265 DAOOVC010000000
VERSION     DAOOVC010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14571_length_3485_cov_5.219242"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLM79_18130"
     CDS             complement(<1..658)
                     /locus_tag="PLM79_18130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV88280.1"
                     /translation="MRPGSNSGEELRPPGVWESIRELLLGVRRPLDCIQVEVTSRCPG
                     RCLYCPHTTMRERWLSRDMDMETFRHLWPLMRRSARVHLQGWGEPLLNPFFFDMAALA
                     RKAGCAVSTTTCGLLMDETLAEKLVESGVDILAFSLAGTDEAGNASRRGVDFERVCRA
                     VSLLQRIRKSRMGVHLEIHFAYLMLASNLEAVKGLPRLMEELGVHASVISTLDYLPGP
                     E"
     gene            complement(674..1519)
                     /locus_tag="PLM79_18135"
     CDS             complement(674..1519)
                     /locus_tag="PLM79_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88281.1"
                     /translation="MILPGLLARVTAEAVVPEQVIPYVCSVTDSKARMIEDCVAFESP
                     GELVLVAYPLHNPRDEIAMARAAGRALQTPGIEKFTVIGPARPQAAPESSPAAQDWYY
                     TVPLPPPPPGQKLRNLLRRASRELRIERGAGWREDHAHLVRFYLDSRPLDPGTRWIFN
                     RLPRYLAASPGSLLLSARRADGRLAGFAVGEYSSLRTAFFMFCFRAPDGAPPGTADLL
                     LHRLLEEGSNRGQVQMNLGLGVNPGIRFFKGKWGAVPFLPCVQVSWVPEAPGFLARLR
                     KILRR"
     gene            complement(1516..3207)
                     /locus_tag="PLM79_18140"
     CDS             complement(1516..3207)
                     /locus_tag="PLM79_18140"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOV88282.1"
                     /translation="MASLDFQAVLNAAVVPEHSVKFMQAVSGGEPFLVGAYLFLAAED
                     WLLAVGYPLWGDYSPESFEEALAGALKRTRATDCWAICPTLPDRLREHRCEQDQYFAL
                     PVGDPWPQRLLRLAERAAASLEVEEGGTFTAAHRKLWAEFTGRVALPPNVKELYARTE
                     HALGRAEGLVLLNAWDSRGRLAACLLVDTAPRRFSSYLLGAHSRVHYVPYASDLLFSE
                     MVRMARRLDKDFMHLGLGVNDGIRRFKTKWGGKPVYPYEMAQWREKPGARAAVDDFMR
                     AWVRSSLSSMSKREYFASLPPQRRFAMLWEMERDGRRSWIGGTAHFFCYSFEASFREL
                     FEKVDTVLFEGPLDRGSLDRVSEIGHNPEPEAPRLVDLLSRKEIERLERVVCGPSGLV
                     ARLLGLRCPNPANVGDLLSRARPWLAFFSLWTSFLARQGWTQSVDLEAWQIARDMGKT
                     VLGMETIPEQIRTLESIPLERIVNYLRRCGRWKGNIRRNVRAYLRGDLEGMMGTSTEF
                     PSRTEHIIHHRDRTFLERMMPFIREGRCAVFVGSAHLLNLRPMLAEAGFTLRKCR"
BASE COUNT          718 a         1114 c         1084 g          569 t
ORIGIN      
        1 attccggccc gggaaggtag tccagggtgc tgatcaccga tgcgtggacc cccagctcct
       61 ccatgagtcg aggcaggccc ttcacggcct ccaggttgga agccagcatg aggtaggcaa
      121 aatggatctc gagatgaacg cccatacgag acttcctgat ccgctgcagc agggagacgg
      181 ctcggcagac ccgttcgaag tccacccccc ggcgggatgc gttgccggcc tcgtccgtgc
      241 ccgccaggga aaaggccagg atatccaccc cgctttccac gagtttctcc gccagggtct
      301 cgtccatgag gagtccacag gtagtggtgg aaacggcaca accggccttt cgagccaatg
      361 cggccatgtc gaagaagaag gggttgagga ggggttcgcc ccaaccctgg aggtgcactc
      421 gagccgagcg gcgcatcaac ggccacaggt gacggaaggt ttccatatcc atgtcccggg
      481 aaagccatcg ttccctcatg gtggtatggg gacagtatag gcaacgcccg ggacatcgcg
      541 aggtcacctc cacctggata caatccaggg gacggcggac ccccagcaac agctcccgga
      601 tgctttccca gactcctggg gggcgaagtt cttctcccga attcgaaccc ggtctcatct
      661 cgatccgtct ctctcagcgg cgaagaattt ttcgcagccg tgccaaaaaa ccaggtgcct
      721 cgggcaccca ggaaacctga acacaaggaa gaaaaggaac cgccccccat ttgcccttga
      781 agaaccgaat tcccgggttc acccccaaac ccaggttcat ctgcacctgg ccccgattcg
      841 agccttcttc gagaagcctg tggaggagga gatcggcggt tcccgggggg gctccgtcgg
      901 gggcccgaaa gcaaaacatg aaaaacgccg ttcgcaggga ggaatactcc ccgacggcaa
      961 aaccggcaag acgaccgtcc gcgcggcggg ccgagagaag caggcttccg ggagacgcag
     1021 ccaggtaccg gggcaaccgg ttgaagatcc accgggttcc cggatcgaga ggcctcgaat
     1081 ccaggtaaaa ccgcaccagg tgtgcgtgat cctccctcca cccggcgccc cgttcgatcc
     1141 gaagctcccg ggaagctcgg cgaagaaggt tgcgcagctt ttgacccggg ggcggcgggg
     1201 gcaacggcac cgtgtaatac cagtcttggg ccgcagggct gctttccgga gctgcctgag
     1261 gccttgccgg gccgatgacc gtgaacttct cgatgcccgg ggtttggagg gcccggccgg
     1321 cggcccgggc catggcaatc tcatcccggg gattgtggag ggggtaggcc accaggacca
     1381 actcccccgg ggattcaaag gccacgcagt cctcgatcat ccgggccttg gaatccgtta
     1441 ccgagcagac atagggaatg acctgctcgg ggacgacggc ttcggcggtg accctggcca
     1501 acagacccgg aagtatcatc ggcatttcct aagggtgaac ccggcctcgg ccaacatggg
     1561 gcgaaggttc agcaggtggg ccgacccgac gaacaccgcg caacgtccct cccggataaa
     1621 aggcatcatt cgttccagaa acgtccggtc ccggtggtgg atgatgtgct ctgttcggga
     1681 tgggaattcc gtgctggtac ccatcatccc ctcgaggtct ccccgcagat acgcgcgcac
     1741 attccgccgg atgttcccct tccaccgacc gcaccgcctc aagtagttca caatccgctc
     1801 cagggggatg ctctcgaggg tccggatctg ttccgggatc gtttccatgc ccaacacggt
     1861 ttttcccatg tcgcgggcga tttgccatgc ttccagatcc acggattggg tccaaccttg
     1921 acgggccaga aagctggtcc acagggaaaa gaaggccagc cacggacgcg cccgggacaa
     1981 aaggtccccc acgttggcgg gattgggaca tcgcagtccc aggagtcggg ccaccaggcc
     2041 cgaggggccg cacacgaccc gttccagccg ctcaatctct ttccgactca gcaggtccac
     2101 aagcctgggg gcctcgggtt cgggattgtg cccgatctcc gacactcggt cgagactccc
     2161 ccggtccagg ggaccctcga agaggaccgt gtccaccttc tcgaaaagct ctcgaaaaga
     2221 agcctcgaag ctgtagcaga aaaaatgggc cgttcctccg atccacgaac gccgcccgtc
     2281 cctttccatt tcccagagca tggcaaatcg cctctgaggg ggaagggatg caaagtactc
     2341 ccgtttggac atggacgaaa gagacgaccg aacccaggcc cgcatgaaat cgtccaccgc
     2401 ggcccgggcc cccggctttt cccgccattg agccatctca taggggtaga cgggcttgcc
     2461 tccccacttg gtcttgaaac gacggatccc gtcgttgact ccgaggccca ggtgcatgaa
     2521 atccttgtcc aggcgccggg ccattcgaac catctcggaa aagagcaggt cggaggcgta
     2581 aggcacgtaa tggacccggg agtgggctcc cagcaggtag ctcgagaagc ggcgcggggc
     2641 ggtatccacc aggaggcagg ccgccagacg gccccgagaa tcccatgcat tgagcaggac
     2701 cagcccctcc gcccgcccca gggcatgttc cgtccgggcg taaagctcct tcacgttggg
     2761 cggcaaagca acccgccccg tgaattcggc ccagagcttt cgatgagcgg cggtgaaagt
     2821 cccgccctcc tccacctcga gggacgcggc cgctcgctcc gccaggcgca gcaaccgttg
     2881 gggccatggg tccccaacag gcagggcgaa gtattggtcc tgttcgcaac ggtgttcccg
     2941 gagacgatcc ggcaaggtcg gacaaatggc ccagcagtcg gtcgcccggg ttcgttttag
     3001 agcacccgcc aacgcttcct cgaaggattc ggggctgtag tctccccaca gggggtaccc
     3061 cacggccaga agccagtctt cggcggcaag aaagagatag gccccaacca gaaacggctc
     3121 tccgcccgac acggcctgca tgaatttcac cgaatgctcc ggaaccacgg cagcgttcag
     3181 gacggcttga aaatccaagg aggccaaggc gattctccgt tttcacgggt caaagcaccg
     3241 ggaccgacgg gcggcagggc gaaaggcatc ccctcggccg ggacgtgtcg accatgaaac
     3301 cttatgaact ctatcagaac gagcgggatg tgagaagggc ggaaggccga ggaaagattc
     3361 caggtcgaag atgcggcgat gccccggcgg gcgcgctcct tggggagcac gaaggcccct
     3421 tatggccggg ggggtgtggg agggtattcc aggctgtctc ggatggctgc cgagccttcc
     3481 gccgt
//
LOCUS       DAOOVC010000266         3482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14588_length_3482_cov_4.211847, whole genome shotgun sequence.
ACCESSION   DAOOVC010000266 DAOOVC010000000
VERSION     DAOOVC010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14588_length_3482_cov_4.211847"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLM79_18145"
     CDS             complement(<1..731)
                     /locus_tag="PLM79_18145"
                     /inference="COORDINATES: protein motif:HMM:NF019313.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HOV88283.1"
                     /translation="MWSDRETEHDFLGYQSYVSVLAEVCMHKDLAPLTLGIFGSWGSG
                     KSSLMSMLKLEIEGSAHASKTKTLWFNAWRYEGREEAQSALIHAVLSKLAEDKTLFQD
                     AKDIFDRLKKGASILKLGKHITKSLMTLTPDIAGFIDCFREESEKIAETMEHFDKDFK
                     QLLQRANIERIVVFIDDLDRCSSQKVIETFETIKLFLNTPECTFVIGADPDKIEESVG
                     EIYKVTETKRKKDYLEKIIQIPFNIP"
     gene            complement(750..3308)
                     /locus_tag="PLM79_18150"
     CDS             complement(750..3308)
                     /locus_tag="PLM79_18150"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOV88284.1"
                     /translation="MLALYHLFSGVSAKDLADVEMLLQTTGVPQAPKACRAVLVGTAL
                     SPGIPHTKPDGTIIHTLWGELAWQLGGQKGYALVANNDANGSSPGSVILSELFKMCSP
                     CLILIDEWVAYARQTYGKIDLPGGSFDSNMTFAQAITEATRATPGTMLVASIPASQIE
                     IGGEGGREALARIQNTFSRMESPWRPASAEESYEIVRRRLFEPIADPELFATRDAVIR
                     AFGQAYRQAGSDFPQGVDEADYQRRMTNAYPIHPELFDRLYEDWSSLERFQRTRGVLR
                     LMASVISELWERQDSSLLILPASIPMDAPVVQSELTRYLEDNWRPIMEQDVDGPEAVP
                     LRIDRDVPTLGRYSATRRVARTIFLGSAPTVKSKNPGIDDRRVKLGCFQPGENVATFG
                     DALRRLTGSKARHLYVDGSRYWFSTQPSVTGLADDRAAQISIDDVWEELKTRLRSDRQ
                     RGEFAAVHVTPASSGDVPDEMEARMVILGPTQRHTSKAKDSQALNSASEILNARGSSP
                     RFYKNMLVFLAPDQARLNDLEEAIRQYLAWNSIWEHRETLNLDAFQSSLARTRREQAT
                     KTVDSRILETYFWLLYPGQPDPQGPVEWQESRLQGLEPLAVRASRKLVNDGNLMNQFS
                     AINLRLELDRCNLWKDVDHLSVKKLWEYFASYIYLPRLTGSGVLTKAIQEGVALVTWS
                     EAFVYAERWDESACRYLGLKIGQHTSVILDGQSVIVKPSAAKAQLEAEKKPSVQPPGT
                     AQPGGTGGTQPGPSPIPIPGGGGVTITIEPTKSIAPRRFYASVKLDPMRISGNAGKIA
                     EEVVQHLSSLLGANVDVTLEIQVKVPEGVPENIVRTVTENCRTLKFSQYGFEEE"
BASE COUNT          717 a          982 c          915 g          868 t
ORIGIN      
        1 ggaatgttga acgggatctg aatgatcttc tcaaggtagt ccttcttgcg tttggtctcg
       61 gtaaccttat aaatttcacc caccgactcc tcgatcttat ctggatcagc gccgattacg
      121 aaagtgcatt ctggggtatt gagaaacagt ttgattgttt caaacgtctc gatcaccttc
      181 tggctggagc accgatcgag gtcatcaatg aagacgacaa tgcgctcgat attcgctctt
      241 tgcagtagct gcttaaagtc cttgtcgaaa tgctccatcg tttcggcaat cttctctgat
      301 tcctcacgga agcaatcgat aaacccggct atgtcaggag ttaacgtcat caatgacttt
      361 gtgatgtgct ttccgagttt gagaatactg gcaccctttt tcagtcgatc gaagatatcc
      421 ttcgcatcct ggaaaagcgt cttatcctca gcgagctttg agagtacggc atggataagg
      481 gcagactggg cctcctcgcg cccttcgtac ctccatgcgt tgaaccatag cgtctttgtc
      541 ttgcttgcgt gtgcactgcc ttcgatttcg agtttgagca tgctcattaa actgctttta
      601 ccgctccccc aagagccaaa tattcccaga gtgagcggtg caaggtcttt atgcatgcac
      661 acttctgcaa gcacggacac gtagctttgg tagccaagaa agtcgtgttc tgtctcacgg
      721 tcagaccaca tagattcctc tctactcctt tactcttctt cgaacccata ctggctgaac
      781 ttcaacgtcc ggcagttctc ggtcactgtt cgaacgatgt tctccggcac accctccggc
      841 actttcacct gtatttcaag cgtgacatca acattcgcgc cgagaagcga agagaggtgc
      901 tgaacgactt cctccgcaat ctttccggca ttgcccgata tccgcatggg gtcaagtttc
      961 acagaggcat agaatcgccg tggagctatg gacttggttg gttcgatagt gattgtcacg
     1021 ccaccaccgc cgggaatggg gattgggctc gggcctggtt gcgttccgcc agtgcctcca
     1081 ggttgagccg taccgggggg ctgaacggag ggcttctttt ctgcctccaa ctgcgccttt
     1141 gctgctgacg gtttgacaat gacactctgg ccgtccagga tgaccgatgt gtgctgtccg
     1201 atcttgagtc ccagatagcg acatgcagat tcatcccatc tctccgcata cacaaaggcc
     1261 tcgctccagg taaccagggc aacgccttcc tgtatggcct ttgtgagcac tccgctgccc
     1321 gtcaggcgtg ggaggtaaat gtaggaggca aagtactccc acagcttctt cacgctcaga
     1381 tggtcaacat ccttccacag attgcatcgg tccagctcaa gacgcaggtt tatcgctgaa
     1441 aactggttca tcagattgcc atcattcacc aacttgcggc tggctcttac ggcaagtggc
     1501 tccagaccct gcagacggct ctcctgccat tctacaggac cttgcggatc tggctggccg
     1561 ggatagagga gccaaaaata ggtctcaaga attcggctat cgaccgtctt ggttgcctgc
     1621 tcacgtctcg ttcgtgccag gctgctctgg aaagcgtcga ggttcagcgt ttcacgatgc
     1681 tcccagatgg aattccaggc caggtactgg cgtatggctt cttccaggtc atttagcctt
     1741 gcctggtcag gagcgagaaa aacgagcatg ttcttgtaga accgtgggct gcttcctctt
     1801 gcattcaaga tttcagaggc agaattcaat gcctgactgt cttttgcttt gcttgtgtgg
     1861 cgctgggttg gtcccaggat caccatccgc gcttccatct cgtctggcac gtcgccgctc
     1921 gatgccggtg ttacatgaac cgccgcaaat tcccccctct gtcgatcaga gcgcaggcgg
     1981 gtcttgagct cttcccatac atcgtcaatt gagatctgag cggcccggtc atcggcaaga
     2041 cccgtgacgc tcggctgtgt tgaaaaccag tagcggctgc cgtccacata gaggtgtctt
     2101 gctttgctgc ccgtaagccg gcgaagcgca tcgccaaagg ttgcgacatt ctcacctggt
     2161 tgaaagcaac ccagcttcac tcgccgatca tcaatacccg ggttcttcga cttcactgtc
     2221 ggagctgagc caagaaagat tgttctggcc acacgacgcg tggctgaata gcgccccagc
     2281 gtcggtacat cgcggtcgat cctaagtgga actgcttccg gcccatcgac gtcttgctcc
     2341 atgatcgggc gccaattgtc ttcgaggtag cgtgtgagct cggactgaac tacgggagca
     2401 tccatgggaa tcgaagcggg aaggatcaga aggcttgaat cctgtctttc ccaaagctcc
     2461 gaaatgactg aagccatgag gcgcagcacg ccgcgggttc gttgaaatcg ctccagactg
     2521 ctccaatctt catagagccg gtcgaaaagc tccggatgga tgggataggc attggtcatg
     2581 cgtctctggt agtcggcttc gtcaacccct tgtgggaaat cgctcccggc ctgacgatag
     2641 gcctgtccga acgcccttat gacagcgtcc cttgttgcga acaattcagg gtcggctatg
     2701 ggctcgaaga ggcgtcgcct aacgatttca tagctttcct cggcgcttgc cgggcgccat
     2761 ggcgattcca tccgactgaa ggtgttctgg attctggcta aagcctctct acctccctca
     2821 ccgccgattt caatttgtga ggcaggaatg ctcgctacca gcatcgttcc cggtgtagcc
     2881 ctggtcgctt cggtaatagc ctgggcgaag gtcatgttgg aatcgaagct gcctcctgga
     2941 agatcgatct tcccgtatgt ctgccgcgcg tatgccaccc attcatcgat caggatgagg
     3001 cagggagagc acatcttgaa aagctcactc agtatgaccg atccggggct gcttccgttt
     3061 gcatcattgt ttgctacaag cgcatagccc ttctgcccgc ccaactgcca ggccagctct
     3121 ccccagagtg tatggatgat tgtgccatcg ggctttgtgt gtgggattcc ggggctcaga
     3181 gcggttccca ccagcacggc ccgacaggct ttgggcgcct gtggtacgcc ggtcgtctgc
     3241 aaaagcatct cgacatcggc aagatctttc gccgaaactc cagagaaaag gtgatagagc
     3301 gccagcatag agtgagtttt cccgccgccg aaattcgtct ggagctctat caccggagct
     3361 ccaccctgat tggtcaggcg caggagcgca tctttcagga gatccgtaag gcctatggtg
     3421 agatacgtgc gccgaaaaaa ctctctggga tcgccatact ccgatgaagc gactcctcgt
     3481 ct
//
LOCUS       DAOOVC010000267         3454 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14742_length_3454_cov_5.618711, whole genome shotgun sequence.
ACCESSION   DAOOVC010000267 DAOOVC010000000
VERSION     DAOOVC010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14742_length_3454_cov_5.618711"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1723
                     /gene="rny"
                     /locus_tag="PLM79_18155"
     CDS             158..1723
                     /gene="rny"
                     /locus_tag="PLM79_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOV88285.1"
                     /translation="MMGYVWNTLILVLGLALGVGIGVAVRYYWLEKRTRRLQEQAKAI
                     VNEARKEAETIKKEAILQAKDSLFQMKAEFEKESKETRRELQNLEKRILQKEENLDKK
                     SEGLDKRESALAKREKSIAQVEKEIEEKEKYLAKLIEEQRKKLENLSGISTQEAKEML
                     VRSIENEARHEAAILIKKIDTEARETADRKAKNIIALAVQRLAGDYVAEKTVSVVNLP
                     NEEMKGRIIGREGRNIRAIEATTGIDLIIDDTPEAVILSGFNPVRREVARVSLERLIS
                     DGRIHPARIEEIVEKVNAEIENSIREAGEQAAFDVGVHGIHPDLIKLIGKLKYRTSYA
                     QNVLQHSREVAFLCGIMAAELGLNEKQAKRAGLLHDIGKAVDHEMEGPHATIGADLAR
                     RYGEAPAIVHAVAAHHEDIPAEDILAVLVQAADALSGARPGARKELLETYVKRLEDLE
                     RIANSFQGIGKAYAIQAGRELRIIVESDQVSDADAVLLSRDIAKRIESELTYPGQIRV
                     TVIREVRSVEYAK"
     gene            1874..3160
                     /gene="tyrS"
                     /locus_tag="PLM79_18160"
     CDS             1874..3160
                     /gene="tyrS"
                     /locus_tag="PLM79_18160"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOV88286.1"
                     /translation="MAEGRNVLDVLTERGFVAQATDTEELRKYLEKPVSCYIGFDPTA
                     DSLHIGSLVPIMSLVHMQRHGHRPIVLVGGGTGLVGDPSGKTEMRQLLSVQRIRKNLQ
                     ALKAQLSGFLDFSDGKALLVNNADWLVDLRYIEFLRDIGRHFSVNRMLAAESYRQRLE
                     TGLNFIEFNYMLLQAYDFHYLAGNYDCLLQMGGNDQWGNIVAGIELIRRKSGGNAHAI
                     TFPLITTASGAKMGKTASGAVWLSEDRTSPFDFFQYWVNTDDRDVMRFLKLYSFLPMA
                     EIRQVENLEGQELNACKTILAYEATAITHGLAKAREAHEAAARVFGRRNLPPDLLPSS
                     TIPREAARDSVGIPATEVDVLRLESGIPVFEIFVEVGLCGSKSAARRLIQQGGAYVNE
                     ERVPDIDFLIEPRHLTPDGILLRAGKKKIHRVTVKD"
BASE COUNT          850 a          863 c         1090 g          651 t
ORIGIN      
        1 ttccagccaa acccctctcg tacgatcaaa tttgtctgcc gcggatgtga ggcgcccccc
       61 aagagaccgt ccgccgcaca gggggagaca ggccccttcg ggcccctccg ttgcgtgacg
      121 gcatgggggg ctgtcggccg taggaaagga tcagggaatg atgggctatg tttggaatac
      181 attgattctg gttttgggtt tagccctggg agttggaatt ggagtggctg tcagatacta
      241 ctggctggag aagcggacca gaaggcttca ggaacaggcg aaagcgatcg tgaacgaggc
      301 gcgcaaagaa gccgagacga tcaaaaagga agccatcctg caagccaaag acagcctttt
      361 tcagatgaag gccgagttcg aaaaggagag caaggagaca cgccgggagc ttcagaacct
      421 ggagaagaga atccttcaga aagaggagaa tctggacaag aaatccgaag gcctggataa
      481 gcgggaatcg gctttggcca agagggaaaa atccattgcc caggtggaga aggaaatcga
      541 ggaaaaagaa aagtacctgg ccaaactgat agaagagcaa agaaagaaac tggagaatct
      601 ttcggggatc tccactcaag aagccaagga gatgctggtg cgctccatcg agaatgaagc
      661 aaggcatgag gcggccatcc tcattaagaa gatcgacacc gaagcccggg agaccgccga
      721 caggaaggca aagaacatca ttgccctggc ggttcaaagg cttgccggag actacgtggc
      781 ggagaaaacc gtctcggtgg tcaacctgcc caatgaagag atgaagggca ggattatcgg
      841 cagagaggga agaaacatcc gggccatcga agcaaccacg gggattgatc tgatcatcga
      901 cgacacaccc gaggcggtaa tcctgtccgg attcaacccc gttcggcgag aggttgcccg
      961 ggtttctctg gaacgtctga tttccgatgg gaggattcat ccggccagaa ttgaagagat
     1021 tgtggaaaaa gtgaacgccg agatcgaaaa ctccattcgt gaggcagggg aacaggccgc
     1081 ttttgatgtc ggcgtgcatg ggattcatcc cgacttgatc aagctgatcg ggaagttgaa
     1141 gtatcgcacc agttacgccc aaaacgtcct gcagcactcc cgagaagtcg cctttctttg
     1201 tggcatcatg gccgcggaac tgggactcaa cgaaaagcag gccaagcgcg cgggcctgct
     1261 ccatgacatt gggaaggccg tggaccatga aatggaagga cctcacgcaa ccattggggc
     1321 tgatttggcg agaagatacg gcgaagcccc ggccatcgtc catgccgtcg ccgctcacca
     1381 tgaggacatc ccggcagaag acattcttgc ggtgttggtc caggctgcgg atgctctgtc
     1441 cggggcccgg ccgggggcca ggaaagaact cttggagacc tatgtgaagc gattggagga
     1501 tctggagcga attgccaatt cgttccaggg aatcgggaaa gcctatgcca tacaggccgg
     1561 gcgggaactg cgaatcatcg tggagagcga ccaggtgagt gatgcggatg ccgtgcttct
     1621 gagccgagac atcgccaagc ggatcgaaag tgaattgacg taccccggcc agattcgagt
     1681 gacggtgatt cgcgaggtca ggtccgtgga atacgcaaag taggggaatt ccgggagaaa
     1741 gggctgaaag gcggcggcgt tcccggaaga tcgggggatg ccctacgaat cccccctcgg
     1801 tcgcgctccc cgggacctgg agagttcccg gcgggctgct gggcgcctcg gggatgagga
     1861 aaggagtcag gatttggcgg aaggaaggaa cgtactggat gttttgaccg aaagagggtt
     1921 tgtggctcaa gctacagaca cggaagaact gaggaaatac ctggaaaagc ccgtctcctg
     1981 ttatattggt tttgacccga cggcggacag tctgcatatc gggagcttgg tgcccatcat
     2041 gtcgttggtg cacatgcagc gtcacggcca ccggcccatt gtgttggttg ggggaggtac
     2101 cggtctcgtg ggagatccca gcggcaagac ggaaatgcgt caactcctga gcgtccaacg
     2161 aatccggaag aacctccaag cccttaaagc tcaactgtcc ggattcctgg atttctccga
     2221 cggcaaggcc ctccttgtga acaatgccga ttggttggtg gatctccgat acatcgagtt
     2281 cctgcgggat atcggaaggc acttcagcgt gaatcgaatg cttgccgcag aaagctaccg
     2341 ccagcgcctg gaaacgggcc tcaatttcat cgagttcaac tacatgttgc tccaggccta
     2401 tgatttccat tatctggcgg gaaactacga ctgcctgctc cagatggggg gcaacgatca
     2461 atgggggaac attgtagcgg ggatcgaact gatccggcga aagagcggag gcaatgccca
     2521 cgccattacc ttccctctca tcacgacggc ttccggagcc aagatgggca agaccgcttc
     2581 gggagcggtc tggttgagcg aagacaggac gagtcctttt gacttctttc agtattgggt
     2641 gaatacggac gaccgggatg tgatgaggtt tctcaagctc tacagcttcc tgcccatggc
     2701 cgaaattcgg caggtggaga acctggaggg acaggaattg aatgcctgca agaccattct
     2761 tgcctatgaa gccacggcca tcacccacgg tctcgccaag gcccgggagg cccatgaagc
     2821 ggccgctcga gtcttcgggc gcaggaacct gcccccggac ctccttccgt ccagcacgat
     2881 tccgcgggaa gcggcacggg attccgtcgg aattcccgcc acagaggtgg atgtccttcg
     2941 attggagtcc gggatcccgg tcttcgagat cttcgtggaa gtcggcctgt gtggttcaaa
     3001 gagcgcggcg cggcgtctga tccagcaagg aggggcttac gtcaacgagg agagggtccc
     3061 cgacatagac tttctgatcg aacccaggca tctgaccccg gacgggatcc tgttgcgggc
     3121 gggaaagaaa aaaatccacc gggtaacggt aaaggattaa gggagttacg gaagaagaga
     3181 ccggaaaggt gatttcttcc tcggtttgtc tcttgacaac gagcacggaa gaggctattt
     3241 aacgcgggct tcgttgatga gggaagaggg ggttcgagaa accaagaatc cccctgggaa
     3301 gtggggccgc accggctcgg aaagaaggtt ctggttcccg cgtccacccc ttgacgacaa
     3361 gatcattaaa cgttacactc aatcgcggct tgtgagctgc cctgttcttt ggaaactaaa
     3421 tagtgggtcg aaggtgctgg gccttttagg gaag
//
LOCUS       DAOOVC010000268         3438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14827_length_3438_cov_4.243571, whole genome shotgun sequence.
ACCESSION   DAOOVC010000268 DAOOVC010000000
VERSION     DAOOVC010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14827_length_3438_cov_4.243571"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..1087
                     /locus_tag="PLM79_18165"
     CDS             308..1087
                     /locus_tag="PLM79_18165"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOV88287.1"
                     /translation="MKKALLVGINDYPGTFCDLMGCVNDVQNMQDLLTSLFGFRKEDI
                     CILTDRDATALNILKALEMLVYDAKPGDQLIFHYSGHGSQVPDTSGDEKDRWDEILCP
                     YDLDWSENVVRDDDLNRIFETVPAGVHVEVFLDSCHSGTGLRALEGEYRKQRFLPVPV
                     EVMGSCTPVKSIKPIKPAKVQVLWAACRSNQYAADALLNGKYGGAFTTFLCDLVRSSQ
                     GTISRKDLLTTLRKGLKANRFAQVPQLETGTKLKKARILAA"
     gene            1273..1614
                     /locus_tag="PLM79_18170"
     CDS             1273..1614
                     /locus_tag="PLM79_18170"
                     /inference="COORDINATES: protein motif:HMM:NF027409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOV88288.1"
                     /translation="MNGDDFFSTSELKVIIISALLAVFGGLAKLFMSQRQLTLSRFLS
                     SSIVSGFSGVMASYLMQCTDLSPAFQSFVIGMSGFAAPTALSAFTVIYERKLGIKLDN
                     ARSKRKKREVR"
     gene            1611..2975
                     /locus_tag="PLM79_18175"
     CDS             1611..2975
                     /locus_tag="PLM79_18175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOV88289.1"
                     /translation="MMKIAIVTPGHPGSGVKSFPPALTAPYLAALATPFAHSIRIYDL
                     AVEPFDFYGPIPDVAMFTTTMAQSDHIFRIARFLKLHGSLIILGGPHATLAYGRDPRI
                     QEIADTVILGEAEKALPQSLRDLIKGGRDALKPLYHTPVNSLSGMPFARLDLLDSRRY
                     YSVTCLIGTRGCPNRCAYCSIKSLYGRKYLRRPVEEVIEEIKYQTSRPGISWTSKRFV
                     TFWDDNPAGDLDWFHTLLEKLVPLKKSWISQMCLNVAESSETVRLMKRSGCLGIFAGI
                     ESVNPESLKGQNKNRINRIDRYTEQCHTFLRHGIQVIGAFMFGFDEDTPRSLTQDTLS
                     LATRMGLTLMQCHLNTPYPHLGYYETLLREGRLLTEEAKYFNGYTVVHRPSRIHPALL
                     QDAFLEVRQRFYSLPCMLHRMVQHRVADWPKFLFWQLLFRKPNYSSIPNVDVNLWHEH
                     LNTL"
     gene            2990..>3438
                     /locus_tag="PLM79_18180"
     CDS             2990..>3438
                     /locus_tag="PLM79_18180"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOV88290.1"
                     /translation="MQPTIFIPGIKGTTLVNTNTLDFDTIWSGIQCKFETILDLELCE
                     NTRFEISPKVIIERGDIEDLAYREAVVILERKIKTPVFIFGYDWRKSCAENGRRLKAF
                     VDYLQEKLSISCFNFLTHSMGGMVFSCYLKELRGNYETVDHAVMTVCP"
BASE COUNT          815 a         1041 c          807 g          775 t
ORIGIN      
        1 atgcgaggaa tccttctacg cagccagcac ccgctcctat caccggccgg gatgctacta
       61 cgcaaaatct gcgaccacgc gtttctcttc gcgggaagaa gccgccaaag cagggctttc
      121 accctgctcg gcctgccttc cggattattc acggacaaaa atatgagccg caaagacgca
      181 aagagcgcaa agaaattccc ctatgtttcc ctcttagcgt ccttcgcgtc tttgcggttc
      241 aaaggttttt ccacagcagt acgtctgatt ccgtcctgca agcaaagcac gcaaagggag
      301 gatagtcatg aagaaagcac ttcttgtagg catcaacgat taccctggaa cattctgtga
      361 cctcatgggc tgcgtcaacg acgttcaaaa catgcaggat cttctgactt cccttttcgg
      421 tttcagaaag gaagacatct gcattctgac cgacagagac gcaaccgccc tgaacatcct
      481 caaagccctc gagatgctgg tctacgatgc aaaaccaggc gatcagctta tcttccacta
      541 ctccgggcat ggctcccagg taccggacac atccggagat gaaaaggacc ggtgggatga
      601 aatcttgtgc ccctacgacc ttgactggag tgaaaacgtc gttcgagacg acgatttgaa
      661 tcggatcttc gagaccgttc ccgcgggcgt ccatgtggaa gtcttcctcg attcctgcca
      721 ttccggcacc ggccttagag cccttgaagg cgaataccga aaacagcgtt tcctccccgt
      781 gcccgtggag gtaatgggct cctgcactcc agtgaaatca atcaaaccca tcaagccggc
      841 aaaggtacag gtcctctggg ccgcatgccg gtcaaaccag tatgcagcgg atgctttgct
      901 gaacggcaaa tacggcggag cattcaccac cttcctgtgc gacctcgtga gaagctccca
      961 aggcaccatt tcgcgcaagg atctcctcac cacactccga aagggcctaa aggcaaaccg
     1021 cttcgcccag gtccctcagc tcgagaccgg cacgaagcta aagaaggcac gtatccttgc
     1081 cgcatgatcc atcgaccttg cggtcgggtt ttaggtttta agttttaggt tttaggtctg
     1141 agaactcagg ccttggcttt catgtctcgg ataccggtgc tacaggctga ccactccaac
     1201 acgaagccag gaacctcacg cctgaactta aaacctaaaa cctaaaactt aaaacccttc
     1261 tggagttcta acatgaacgg agacgatttc ttctcgacca gtgagctgaa ggtcatcatc
     1321 atttcagccc ttctcgccgt ctttggcggg cttgccaagc tcttcatgtc ccaaaggcag
     1381 ctcacgctct cgcggtttct ctccagctcg atcgtctccg gcttcagcgg agtcatggca
     1441 agctacctca tgcagtgcac ggatctttcc ccggctttcc agagtttcgt catcggaatg
     1501 tcaggttttg cagctcccac tgcactctct gcattcaccg taatctatga aaggaagctc
     1561 ggaatcaagc tcgacaatgc caggtcgaaa agaaagaagc gggaggtgag atgatgaaga
     1621 tagccattgt aactccaggg catccgggat cgggagtgaa gtcgtttccc ccggcgctca
     1681 cggctccgta tctcgcggca ctggccaccc catttgcaca cagcattcgg atctacgatc
     1741 ttgctgtcga acccttcgac ttctatggcc ccattcccga cgtcgccatg ttcacaacga
     1801 ccatggcgca gtcggaccat atcttccgta tcgcgaggtt cctcaagctc catggttccc
     1861 tgatcatcct tggcggtcct catgcaacgc ttgcctatgg tcgtgatccc cgcattcagg
     1921 aaattgccga tacggtcatc ctgggcgagg cggaaaaagc attgcctcag tcgctgcggg
     1981 atctcatcaa aggaggccgt gacgctctaa aacctctcta ccataccccc gttaattccc
     2041 tctccggcat gccttttgcc cgcctcgatc tcctggattc tcgcagatac tattccgtaa
     2101 cctgcctcat cggcacccgt ggctgtccca accgatgcgc ttactgttcc ataaaatccc
     2161 tctacggccg gaaatacctg agacggccgg ttgaagaggt catagaagag atcaagtacc
     2221 agacctctcg gcccggaatt tcctggacca gcaaaaggtt tgttaccttc tgggatgata
     2281 accctgcagg agatctcgac tggttccata cactcctgga aaagctcgtc ccgctcaaga
     2341 aatcgtggat ctcccagatg tgcctcaatg ttgcagaaag tagcgaaacc gttcgcctca
     2401 tgaagcgatc gggatgtctc gggatctttg ccggtatcga atcggttaac cccgaaagcc
     2461 tcaagggaca aaacaaaaac cggataaacc gaatcgatcg gtacaccgag cagtgccaca
     2521 cattccttcg ccacggaatc caggtcatag gggcattcat gttcggattc gatgaggaca
     2581 ctccacgctc cctcacccag gataccctct cgctcgccac ccgcatgggt ctcactctca
     2641 tgcagtgcca cttgaatact ccatatcccc accttggcta ttatgagacg cttctccgcg
     2701 aaggccggct tttaaccgag gaagcaaagt atttcaatgg ctacacggta gtgcaccgtc
     2761 catcgaggat ccaccccgct cttcttcagg acgccttcct ggaggtccgt caacgattct
     2821 actcgctccc ctgtatgctg catcgcatgg tgcagcaccg tgttgcggac tggccaaagt
     2881 tccttttctg gcaactactc ttccgcaaac ccaactactc gagcatcccc aacgtggacg
     2941 taaacctctg gcacgagcac ctgaacaccc tttgagagag gataagagca tgcagccgac
     3001 cattttcatt cccggcatca agggaaccac cctggtcaac accaacaccc tcgactttga
     3061 cacgatctgg tcgggcatcc agtgcaagtt cgaaaccatc ctggatctcg agctctgcga
     3121 gaacacccgc tttgagatct cgccaaaggt gatcattgag cgaggagaca tagaggacct
     3181 tgcctacaga gaagcggtgg tcattctcga gcggaagatc aaaactcccg tcttcatctt
     3241 cggctatgac tggcgcaaat cctgcgccga aaacggtcga agactcaagg cctttgtcga
     3301 ttacctccag gagaagctct ccatctcctg cttcaatttc ctcacccaca gcatgggagg
     3361 catggtcttt tcctgttatt taaaagaact tcgcggcaat tacgaaacag tagaccatgc
     3421 tgtcatgacg gtctgccc
//
LOCUS       DAOOVC010000269         3433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_14850_length_3433_cov_5.024275, whole genome shotgun sequence.
ACCESSION   DAOOVC010000269 DAOOVC010000000
VERSION     DAOOVC010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14850_length_3433_cov_5.024275"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLM79_18185"
     CDS             <1..685
                     /locus_tag="PLM79_18185"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOV88291.1"
                     /translation="SILYENMTGRVPLTYIDGQWLLKSPELQGSLSVGYLRVKRVMDM
                     VLSIFFLALTAPLFLIIPIFIKLDSEGPVFFVMERLGHLRKPFKCYKFRTMIHDAESR
                     YGPMWATENDPRITRVGRILRKARLDELPQLWNVLKGDMTFVGPRPIREHFAAKLAKE
                     IPFYELRFCIKPGLTGWAQVSHDYAGTNEGHLEKFQYELFYIRNMSLLLDALTIFKTV
                     KTLFKRTGL"
     gene            783..1988
                     /locus_tag="PLM79_18190"
     CDS             783..1988
                     /locus_tag="PLM79_18190"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOV88292.1"
                     /translation="MAYQSTAGAKDEINLLDFFVVLLKRKKFILGGGLGVAVLSFVLC
                     MVMSPMYQGISQLMPPQQSGASAAAQMLGQLGGIAGMVLGGGGAGPVSVSDLYVQILQ
                     SPAVLDPIIDRFDLKKLYGYDNYVDTRRNLVDNILTVESDLKSGIIFLGVYDVDPKRA
                     AEIANAMVATLKDLLENLATSEAGKRRVFFEGQLKKAHENLKEAEAALQTFAETTGAI
                     KIDDQASAVLQGIANLRAQVAAKEVQLKVMKTYATPSNPDLKMVEQELRGLNEQLKKL
                     EEKEEVPSTDAIIPTGQIPTVGTEYLRRMRDFKYTELLYELLVKQYEAARLDEARESA
                     VVQAIYPAKPPDKKAKPMMILMVVLGGGLGCFLFTVTAVVVETMRKASRNPENEGRFR
                     DMKRFLRYA"
     gene            complement(2017..2895)
                     /locus_tag="PLM79_18195"
     CDS             complement(2017..2895)
                     /locus_tag="PLM79_18195"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOV88293.1"
                     /translation="MEILLVGFAVLMGILVNVALLTTVLFWYETAWGPHRNHLERLSG
                     GRVGALLCRTAFLSIFSMLAVVLLYPWGYRQRAQRTKADPSCPLPPVVLIHGLYHNAS
                     GWFLYRRWLRKAGFANLYAVNYSSWNTSFEEALGKVARVVEEVAGEFPGQPVMLVGHS
                     LGGLLSRALVEGYGPKLPVSAVVTLGTPHHGSKLAVFGVGRLARSLVYGGPLIQSLGQ
                     SLEKDSADCRGRCLALYSPLDNMVLPNEALHPPDSWQHEETFPLSHIGMLFHRPTAVR
                     TIRHLRECSTRDTPQP"
     gene            complement(2904..>3433)
                     /locus_tag="PLM79_18200"
     CDS             complement(2904..>3433)
                     /locus_tag="PLM79_18200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOV88294.1"
                     /translation="PLVEALCGILPGMESGRVAREIRKLWQEQLESAREIDGASPLLR
                     SLKELGIRLGVISNIWHPFYQGLAQRRPSLVETLDYKILSYRKGLMKPSTELYREALE
                     ACGEPPQDCWMVGDTYELDVAPAMHLGMRTVWVLNRPEKEKVSLVGVLRGEKPSPDWV
                     TGDLGEVERFFSRKG"
BASE COUNT          713 a         1025 c         1036 g          659 t
ORIGIN      
        1 ctcgatcctg tacgagaaca tgaccggcag ggttcccctg acgtatatcg acggtcagtg
       61 gctcttgaag agcccggaac tccagggatc tctcagcgtc ggttacctcc gggtgaagcg
      121 ggtcatggac atggtgctct ccatcttttt tctggccttg acggctcccc tgttcctgat
      181 catcccgatt ttcatcaagc tggacagcga ggggccggta ttctttgtca tggagcggtt
      241 ggggcacctg cgcaagccgt tcaagtgcta caaattccga accatgatcc acgatgcgga
      301 aagtcggtac ggtcccatgt gggccaccga gaacgacccc agaattaccc gggtcgggcg
      361 catcctgcgc aaggcccggt tggacgagct gccgcagttg tggaatgtgc tcaagggcga
      421 catgactttt gtcggcccga ggcccatccg agagcatttt gccgccaagc tggccaagga
      481 aatccctttt tatgaactgc gcttctgcat caagccgggc ctcaccgggt gggcccaggt
      541 cagtcacgac tacgcgggga ccaatgaagg gcacctggag aaatttcaat acgagctgtt
      601 ctacattcgc aacatgtccc tgctcctcga tgccctgacc atcttcaaga ccgtgaagac
      661 cctcttcaaa cgcacgggcc tgtgagggac tcttgggaaa acaggtggga ccgcccccgt
      721 gggtgtacgg gggaggagtc cgaatcccga ttcattcgat gagacatcct tgaggaatgc
      781 ttatggctta ccagtcgacg gcgggggcaa aggacgaaat caaccttctg gatttcttcg
      841 tggtactcct caaacgcaaa aagttcattc tgggtggcgg gttgggggtc gcggttctca
      901 gttttgtgct ttgcatggtc atgtccccca tgtaccaggg catatcccag ctcatgcctc
      961 cccagcaaag cggcgcttcg gcggcggccc agatgctggg ccagttgggg ggcattgcgg
     1021 ggatggttct gggcggcggg ggtgcggggc cggtctcggt gagcgatctg tacgtccaga
     1081 tccttcagag tcctgccgtc ctcgatccca tcatcgatcg tttcgatctg aagaaactct
     1141 acggctacga caattacgtg gatacgcgcc gcaacctcgt cgacaacatc ctgacggtgg
     1201 agtcggatct aaagagcggc atcatctttt tgggggtcta cgacgtggat cccaagaggg
     1261 ccgccgagat cgccaacgcc atggtggcga ccctcaagga tctcctggag aatctcgcaa
     1321 cctccgaggc aggcaagagg agggtcttct tcgaagggca actgaagaaa gcccatgaaa
     1381 acctgaagga agccgaggcg gcgttgcaga cttttgcaga gaccaccggc gccatcaaga
     1441 tcgacgacca ggcttcggcg gtgctccagg gcatcgcgaa cctgagggcc caggtggcgg
     1501 ccaaagaggt gcagctcaag gtcatgaaga cctacgcaac ccccagcaat ccggacctga
     1561 aaatggtgga acaggagctc agggggttga acgagcagct gaaaaagttg gaggaaaaag
     1621 aagaagttcc ttccaccgat gccatcatcc ccaccgggca gatcccaacg gtgggaacgg
     1681 aatatcttcg caggatgagg gacttcaagt atacggaact gctttacgaa ttgctggtca
     1741 agcaatacga ggcggcaaga ctggacgagg cgcgggagtc cgccgtcgtg caggccatct
     1801 acccggccaa gccccccgac aagaaggcca agcccatgat gatcctgatg gtggtcctgg
     1861 gcgggggcct gggttgtttc ctcttcaccg tcacggcggt ggtcgtcgaa accatgcgca
     1921 aggcgtccag gaaccccgag aacgagggtc gttttcggga catgaagcgg ttcctccggt
     1981 acgcctgacc aactctccca ttccccgaac cgacccttac ggctgcggtg tatctcgagt
     2041 cgagcactcc cggaggtggc ggatcgtccg gactgccgtg gggcggtgaa agagcatccc
     2101 gatgtgggaa aggggaaagg tctcttcgtg ctgccaggag tccgggggat ggagcgcctc
     2161 attgggcaga accatgttgt ccaggggcga gtagagggcc aggcagcgac cccggcaatc
     2221 tgcggagtcc ttttccaggg attggcccag ggactggatc agaggccctc catagaccag
     2281 gctccgggcg agcctgccca ccccgaaaac cgccagcttg cttccatggt gaggagtccc
     2341 cagggtgacg acggccgaga cgggaagctt cggaccgtat ccctcaacaa gggctcgact
     2401 caaaagcccc cccaggctgt ggcccaccag catcacgggt tgaccgggaa actccccggc
     2461 gacttcttcc accacgcggg ccactttccc cagcgcctcc tcgaaggatg tgttccaact
     2521 cgaatagttc acggcataaa gattggcgaa cccggctttg cggagccacc gtcggtagag
     2581 aaaccagccg ctcgcattgt gatacaaccc gtggatcaag accaccgggg gcaggggaca
     2641 ggagggatcg gccttggtgc gttgtgccct ttgccggtat ccccagggat acaaaaggac
     2701 tacggccagc atggaaaaga tgctcaggaa ggccgttcgg caaaggaggg ctcccacgcg
     2761 gccgccgctc agccgttcca ggtgattgcg gtgcggcccc caggccgtct cgtaccagaa
     2821 gagcaccgtg gtgagaagcg ccacgttgac caggattccc atgaggactg caaacccgac
     2881 cagaagaatt tccatccttt ggttcatcct ttccttgaaa agaagcgttc cacctccccc
     2941 aggtctccgg tcacccagtc cggggacggc ttttctcccc gcaatacccc caccaggctg
     3001 accttttcct tttccgggcg gtttaagacc cagacggtgc gcatgcccag gtgcatggcc
     3061 ggagccacgt ccagttcgta ggtgtctccc accatccaac agtcctgcgg cggttctcca
     3121 cacgcctcga gagcttcccg gtagagctcc gtcgagggct tcatcagccc cttgcggtag
     3181 ctcaggatct tgtagtccag ggtctcgacc agggagggcc gcctttgtgc caggccctgg
     3241 tagaagggat gccagatgtt tgagatgacc cccaatcgaa tccccagctc ttttaggctc
     3301 cgcaacaggg gagaggcccc gtcgatctcc cgggccgatt ccagctgctc ttgccagagc
     3361 ttccgaattt cccgggcgac ccggcccgat tccattcccg gaaggattcc gcatagggcc
     3421 tcgaccaggg gat
//
LOCUS       DAOOVC010000270         3368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15213_length_3368_cov_5.670691, whole genome shotgun sequence.
ACCESSION   DAOOVC010000270 DAOOVC010000000
VERSION     DAOOVC010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15213_length_3368_cov_5.670691"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLM79_18205"
     CDS             <1..343
                     /locus_tag="PLM79_18205"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOV88295.1"
                     /translation="KPVPPKTASAVPSSQGPPPTSAPGPRKVDPPGGTRLFAVQLGSF
                     REKHNAEELKARLSRKGYSVVIKPQKHPTLGSLYVVQLEPVSDERKANTLMAQVRQES
                     KVNPVIIRVAP"
     gene            complement(512..1264)
                     /gene="surE"
                     /locus_tag="PLM79_18210"
     CDS             complement(512..1264)
                     /gene="surE"
                     /locus_tag="PLM79_18210"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOV88296.1"
                     /translation="MRILLTNDDGIYAKGIEALFHCLCKDHEVTVVAPTTEQSAIGHA
                     ITFLNPLRVTPVRRNGSFFGLALDGTPADCVKIAVTTLLDPPPRMVVSGINLGANVGV
                     NVIYSGTVSAATEAALLGIPAVAVSIDSFDTNDFSAATSFIPRLVRLIEEHGLPPGVS
                     LNVNVPSVPASRVRGVRITHQGRMQCVETYDRRVDPRNHVYYWLCNRDILPDSEPDAD
                     SAVLTENCISVTPIQHDLTHYGMIEVLKEWKL"
     gene            complement(1242..1829)
                     /locus_tag="PLM79_18215"
     CDS             complement(1242..1829)
                     /locus_tag="PLM79_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOV88297.1"
                     /translation="MKTDRYEIRLSGAGGQGIITAGMILAEAAGIHEGKFVCQTQSYG
                     PEARGGASKTEIVLSSREIDYPKATQPDVLLAMNQKSCDTYSFDLKPSALLIVDSTFV
                     RQLPTSRALGIPFTRLARSRLGSEVYASVIALGALVTISGVVSLKSIEAAVLGRAPHG
                     TGEINRKALHLGVGEAKKVLKQREGEEVHENSTDE"
     gene            complement(1826..2680)
                     /locus_tag="PLM79_18220"
     CDS             complement(1826..2680)
                     /locus_tag="PLM79_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOV88298.1"
                     /translation="MAEVTKLIHKYLRHDKKFPHVWCPGCGNGILLGSLIRAIDRTGY
                     EKDQIVLVSGIGCSGRLTVYVDFNTLHTTHGRALTFATGVKLARPELQVIVVMGDGDA
                     TAVGGNHFIHAARRNLNLTAIIVNNQIFGMTGGQYSPTTPYGARATTALYGNIEHTFS
                     IAELAVTAGASMVARGTVYHAAMLDDLIEKAILKKGFSVVEVISHCHTQYGQRNRLGG
                     AVEMLRAQKEAAVRVEKAGAMTEEELKGKFTVGVLVDRDLPIYTQELKRIRETAREAW
                     SGPSGGTN"
     gene            complement(2659..3291)
                     /locus_tag="PLM79_18225"
     CDS             complement(2659..3291)
                     /locus_tag="PLM79_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88299.1"
                     /translation="MSSTLVSKLTQQALRLKQLDQEVVKSVVEWRTDDLFDFLNEFNE
                     LKKDIRLSMIDASVIKIDNAMAQTVLQRVSLGPPFQTDRVIEAMTGERHSDFEMAGFS
                     DGELWDLGDEHFYSWFSHYEYVRAMFEIGALIIGISIPKEIDRYVSEVRRCYAFQQYH
                     AVYSMCRTILEAAVRHKCQTQGRLKKSQGNLIDFEGRFERNRKVWRKSPS"
BASE COUNT          637 a         1022 c          972 g          737 t
ORIGIN      
        1 gaagcctgtt cccccaaaga ccgcttccgc tgtcccctcg tcccagggcc ccccgcccac
       61 ctccgccccc ggcccacgga aagtcgaccc ccccggcggg acccgcctgt tcgccgtcca
      121 gttgggctcc tttcgagaaa aacacaacgc cgaagagctc aaggcccgtc ttagcaggaa
      181 gggctattcg gtggtcatca aaccccagaa gcatccgacc ctgggatctc tttacgtggt
      241 gcaactggag cccgtttccg acgaacgcaa agccaacacc ctcatggccc aggttcgtca
      301 ggaatccaag gtgaacccgg tgatcatccg agtcgccccg taggtgcagg ggtcaagtct
      361 cagtttgtct tagcggggag gagacgctgc cgctccccgt tcacccgcct tcgcttcatt
      421 cccccttctc ggttggccgc gagcgaggct ccggctcatg gccgtccttg gggaccaccc
      481 ggtcaaaacc ggggaccccg ggaagccgtg cctacagctt ccattccttg aggacctcga
      541 tcatgccgta gtgggtcagg tcgtgctgga tgggggtgac ggagatgcaa ttttcggtga
      601 gaacggcgga atccgcatcg ggttcggaat cgggaaggat gtcccggttg cacaaccaat
      661 agtaaacgtg gttccgggga tccaccctcc ggtcgtaggt ctccacgcac tgcatcctgc
      721 cctggtgggt gatgcggacc ccccggaccc gggatgccgg gacgctgggc acgttgacgt
      781 tgagagaaac cccggggggg aggccgtgtt cctcgatcaa tcggacgagc cgggggatga
      841 aggaggtggc cgcgctgaaa tcgttcgtgt cgaaggaatc gatggacacc gccacggccg
      901 gaattcccag gagcgccgcc tcggtggcgg cggaaaccgt gccggaatag atgacgttga
      961 ctcctacgtt ggcccccagg ttgatccccg agaccaccat ccggggaggc ggatccagga
     1021 gagtggtgac ggcgatcttg acgcaatcgg caggggttcc gtccagggcc agtccgaaaa
     1081 aactcccgtt tcttcgcacg ggggtcaccc gcagagggtt caggaaggtg atggcgtgcc
     1141 cgatggcgct ctgttccgtg gtgggagcca ccaccgtgac ctcgtgatcc ttgcacaaac
     1201 aatgaaaaag cgcctcgatt cccttggcgt aaattccgtc gtcattcgtc agtagaattc
     1261 tcatggactt cttcgccctc gcgttgcttc aggacttttt ttgcctctcc gacccccagg
     1321 tggagggcct tgcggttgat ttctccggtg ccgtggggag ctctccccag gacggcggcc
     1381 tcgatgcttt tgagggaaac caccccgctg atggttacca gggcgcccag ggcaatcacg
     1441 cttgcgtaca cctcgcttcc caagcggctt cgggccagtc gagtgaacgg gatccccagg
     1501 gcccggctgg tgggaagttg cctcacgaag gtggaatcca caatcaagag ggccgagggt
     1561 ttcaggtcga aggaatacgt gtcgcaggat ttctggttca tggccagaag cacgtccggc
     1621 tgggtcgcct tgggatagtc gatctcccgg gagctgagga cgatctccgt cttgctggcc
     1681 ccgccccggg cttcgggccc gtagctctgg gtctggcaga cgaacttgcc ttcgtggatt
     1741 ccggcggctt cggccaggat catcccggcg gtaatgatcc cctgtccccc cgctccgctc
     1801 aatcgaatct cgtatcgatc cgtcttcaat tcgtccctcc ggacgggccc gaccacgcct
     1861 cgcgggccgt ttcccgaatc cttttcagtt cttgagtata gatcggcagg tcccggtcga
     1921 ccagcacccc gacggtgaat ttgcccttga gttcctcttc cgtcatggcc ccggcctttt
     1981 ccacccggac cgccgcttcc ttctgggctc gaagcatctc cacggccccc cccaaccggt
     2041 tcctttgtcc gtactgggtg tggcagtggc tgatcacctc caccacggaa aacccttttt
     2101 tgagaatggc cttctcgatc aagtcgtcga gcatggccgc atggtacacg gttccccggg
     2161 ccaccatgct cgcccccgcc gtcaccgcca gttccgcaat ggaaaaggtg tgttcgatgt
     2221 tcccgtaaag ggccgtggtg gccctggctc cgtagggagt cgtcggggaa tactggcctc
     2281 ccgtcatccc gaagatctgg ttgttcacga tgatggcggt gaggttcaaa ttcctccggg
     2341 ccgcgtggat gaaatggttg ccgccgacgg ccgtggcgtc cccgtctccc atcaccacaa
     2401 tgacctggag ctccggtcgg gccagcttga ctcccgtggc gaaggtgagg gcccggccgt
     2461 gggtggtgtg cagggtgttg aagtcgacgt aaaccgtgag ccttcccgaa cagccgatgc
     2521 cggataccag gacaatctgg tccttttcat agcccgtgcg gtcaatggcc cgaatgaggg
     2581 atcccagcag aatgccgttg ccgcatcccg ggcaccagac atgggggaat ttcttgtcgt
     2641 gcctcaggta cttgtggatc agcttggtga cttccgccat acttttcggt tcctttcgaa
     2701 acgaccttcg aagtcaatga gatttccttg agacttcttt agcctgcctt gtgtttggca
     2761 cttatggcga acagccgcct caagaatggt gcggcacatg gagtaaacag cgtgatactg
     2821 ctgaaaagca tagcaccttc gaacttctga cacatagcga tcgatttctt tgggaataga
     2881 gatgccgata atgagagcac ctatttcgaa catggctctt acatattcat agtggctgaa
     2941 ccatgagtag aaatgctcat caccgagatc ccagagctca ccatcagaga atccggccat
     3001 ttcaaagtct gaatgtcttt ctcccgtcat tgcctcaatg actcgatctg tctgaaacgg
     3061 tggcccgaga ctcaccctct gcaagacagt ctgcgccatt gcgttgtcta tcttgataac
     3121 tgaggcgtcg atcatcgaaa gcctgatgtc cttctttagt tcgttgaatt cattcaagaa
     3181 atcaaagagg tcatcagttc tccactcgac aactgacttg acgacttctt ggtccagttg
     3241 cttgagtcga agagcctgtt gagtgagttt acttacgagg gttgatgaca tagccattcc
     3301 tttttatgaa tctaacgccc aagctcactg gcggggatgg agcgcagcgg aatccccgtc
     3361 cagtgcag
//
LOCUS       DAOOVC010000271         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15252_length_3361_cov_4.361464, whole genome shotgun sequence.
ACCESSION   DAOOVC010000271 DAOOVC010000000
VERSION     DAOOVC010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15252_length_3361_cov_4.361464"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PLM79_18230"
     CDS             complement(<1..503)
                     /locus_tag="PLM79_18230"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOV88300.1"
                     /translation="MEEKTTVSDAANRLAWLSLKLVPELGNRGILRLVDHFGSPAAVL
                     EASARELSDFRGLKEAARTALLARAFTHPPAQEMEKIRRAGARILTMLDPDYPSNLRV
                     IPDPPAVLFVKGSIEPRDLVAVAVVGSRAASTPGMAFTEGLCRDLAYGGVTVVSGLAV
                     GIDSAAHR"
     gene            906..2243
                     /locus_tag="PLM79_18235"
     CDS             906..2243
                     /locus_tag="PLM79_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOV88301.1"
                     /translation="MVIDRKETFLKSSGKIADPESQSPGVSRPELLAPGGSLEKCVVA
                     FLYGADAVYAGTRRYTLRAQAGNLGWEELAAARHQARLWKRKLYVTVNSFARQEEIRS
                     LPAFLEYLQEIEVDGIILSDPGVLRLAARWAPQVPIHLSTQANTTNAESVRFWADRGV
                     KRINLARELCFEEIRDIRSWVSTELEIFVHGAMCISYSGRCLLSAALNGRSANSGTCT
                     QPCRWSYRLVEEKRPGEYFPIWEDSGGSYIFNSRDLCLLGELGLLLPLGIDAFKVEGR
                     MKGMLYLATVVRAYRAVMDRWARGCALGPAEAEGLEDLGRVSHRPYTRGFLFPSKGPE
                     DAKSAPEVTYLQTHALAGIVRPSPHGYWEEPVGPGDPDSSHIIYLEVRNRLTPGTTLE
                     FLFPDGSIRAHRLESFEDCRGARRDVAHAGTWIRFPVPFPTFPLQVVRMELNS"
     gene            complement(2820..>3361)
                     /locus_tag="PLM79_18240"
     CDS             complement(2820..>3361)
                     /locus_tag="PLM79_18240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOV88302.1"
                     /translation="KPGKNWPEIFASIEKGEIRGLVLIGVNPFELGSPRTRVEGILAR
                     LDFLLCQDALRTPALEYAHVTLPGATFVEKDGTATNCERRVQPMVRSLESPGDARQDF
                     DLLNSLLGLLHPGLEVPGRGAAFSQAAESMAELQGLQIESLPVEGIQWPVSADGRGME
                     RLLPPEEGKARFKFFPARI"
BASE COUNT          590 a         1089 c         1074 g          608 t
ORIGIN      
        1 cggtgggcgg cactgtcgat cccgacggcc aggccgctca ccaccgttac cccgccgtaa
       61 gccaagtccc ggcacaggcc ttccgtgaac gccatccccg gggtagaagc cgcccgggac
      121 cccaccacgg ctaccgccac caagtcccgg ggctcgatgg accccttgac gaacagcacc
      181 gcaggcggat cgggaataac ccgcaggttg gacgggtagt ccgggtccag catggtcagg
      241 attcgagccc ccgcccgccg gatcttttcc atctcctgtg ccggagggtg agtgaaggcc
      301 cgggccagca aagccgttcg cgcagcttct ttgagccccc gaaagtcgga gagttcccgg
      361 gccgaggctt cgagtaccgc cgccggggac ccaaagtggt ccaccagccg gagaatgccc
      421 cggttgccca gttcgggcac cagctttaag gacagccacg caagacgatt tgcggcatcg
      481 gaaaccgtgg tcttttcttc catcccatcc ctgccaggcg atgcgagcaa cttcgggccg
      541 tcacaatccc gctgccggac cattccatcc tggtcgagcg attcgcgggg gatcagcccg
      601 tggtgcccgt gaaggggggt atcccgcagg catggaccct tttccaggac gggaatcctc
      661 cgcccagggg gcctgcaccc cgttgcaagg aaagcccccc tttcccggga atcaccagag
      721 gggattcgtc ccggcccaat cgctccatcc aggttccgtt cttcgagcgg cgaagagtta
      781 ccgcgcggtc cgcacgggat cgtcggggac cacacctcga ggcggtgctg cttgccgttg
      841 tatgctgtgc aatgataagt acagatgctt ttctcatttg tcaaactcat gaacgggttt
      901 ccccagtggt tatcgacagg aaggagactt ttctgaaatc atccgggaag atcgccgatc
      961 cggagtccca gtcacccggg gtatcgaggc ccgagctcct ggctccggga ggaagcctcg
     1021 aaaagtgcgt cgtcgcgttt ctctacgggg ccgacgccgt ctacgcgggc acgcgccggt
     1081 acaccctccg tgcccaggcc ggaaacctgg gttgggagga actggccgcg gctcgtcacc
     1141 aggcccgcct gtggaagagg aagctgtacg ttacggtcaa ctcctttgca cggcaggaag
     1201 aaatccggtc tttgcccgcc tttctcgaat atcttcaaga gatcgaggtg gacggaatta
     1261 tcctgagcga tcccggggtg ctgcgcctgg cggcccggtg ggccccccag gtgcccatcc
     1321 atctcagcac ccaggccaac accaccaacg ccgagagcgt ccgcttctgg gcggaccggg
     1381 gggtcaagag gatcaacctg gcccgggagc tctgcttcga ggaaatccgg gacatccggt
     1441 cctgggtctc caccgagctc gaaatcttcg tccacggggc catgtgcatc tcctattcgg
     1501 gacgctgcct tctcagcgcg gccctaaacg gccgatccgc caactccggg acgtgcactc
     1561 aaccctgccg atggtcctat cgcctggtcg aggagaagcg tcccggcgag tattttccca
     1621 tctgggaaga ttcgggcggc tcatacatct tcaattcccg ggatttgtgc ctcctcgggg
     1681 aactgggact gttgcttcct ctggggatcg atgccttcaa ggtggaaggg cggatgaagg
     1741 gcatgctgta cctggccacg gtggtgcgcg cgtaccgggc agtcatggat cgttgggccc
     1801 gagggtgcgc tttgggcccg gcggaagccg aagggttgga agatctcggg cgcgtgagcc
     1861 accggcccta cacccggggc ttcctctttc cctcgaaggg acccgaggac gcaaaatccg
     1921 cccccgaggt cacctatctc cagactcacg ccctggcggg aatcgttcgc ccgtcccccc
     1981 atggatactg ggaggaaccg gtcggcccgg gagatcccga ttcctcccac attatctacc
     2041 tggaggtgcg aaaccggctc acccctggaa cgaccctcga gtttctcttt cccgacggtt
     2101 ccattcgggc tcatcgcttg gagtctttcg aagactgccg gggagcgcgc cgggacgtgg
     2161 cccacgcggg aacctggatc cggtttcccg ttccctttcc cacctttccc ctccaggtgg
     2221 tccgcatgga gctgaactcg tagggaagca cggccccgga cccgttttcc cgtttccccg
     2281 aatcccgggg ggggcccgga gaatggggcc ttaagacggc atggcagggg agccgagatt
     2341 ttttcgtttt cgaatccctc ggcgccatcc ttccgggtcg ttcctgtacg gacccgaccc
     2401 ggaaaatacc ggcgcagacg ggatcttgcc gggaggggga cggagggtcg tcgtccgggg
     2461 tttgatggat gccgctgggg gtgagtcttc tcgggaagga tgggaggaga acgatccgct
     2521 ccaccgcgtg gaaaggagat gagagacgac cgaaatcccg gtccggtacc tgccgcaccg
     2581 gcggtactcg ctgtctgtga cccggcgcat ccgttcctcg ggccgggtcc tcgatccgaa
     2641 ggggttcgag caccgggggc agggaatcgg gaaggggaac cgagacggga caacttccgg
     2701 gaacgccgcg ggcaggcgca gtcccgcccg cgggccttat gccctggagg ggcgacggtg
     2761 ccccgatcga ccgacagtag gttccgagtg ccttgccgat cggccctgca cccggcgcat
     2821 caaatccttg ccgggaaaaa cttgaaacgt gccttgcctt cctccggggg aagaagtcgt
     2881 tccatgcccc tgccgtcggc cgaaacgggc cattggattc cttccacggg aagggattcg
     2941 atctgtaggc cctgcagctc cgccatggat tccgcggcct gggaaaaagc ggcccctcga
     3001 cccgggacct cgagccccgg gtggagcagc ccgagcaagg aattcagcag atcgaaatct
     3061 tgccgggcgt ccccggggct ctccagggat cggaccatgg gctgcacccg ccgttcacaa
     3121 ttggtggcgg tgccgtcctt ttccacgaag gtggcgccgg gcagagtcac gtgggcatac
     3181 tcgagggcag gggttcgcag ggcgtcctgg cagaggagaa aatccaggcg cgccaggatc
     3241 ccttccaccc gggtcctcgg ggagcccaac tcgaacgggt tgaccccgat gaggaccagt
     3301 ccccgaattt ctcccttctc gatggaggcg aaaatctccg gccagttctt gccgggtttg
     3361 c
//
LOCUS       DAOOVC010000272         3353 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15303_length_3353_cov_5.999697, whole genome shotgun sequence.
ACCESSION   DAOOVC010000272 DAOOVC010000000
VERSION     DAOOVC010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15303_length_3353_cov_5.999697"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLM79_18245"
     CDS             <1..633
                     /locus_tag="PLM79_18245"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOV88303.1"
                     /translation="HVSHEETADITELDALRRRHKGEIEAAGGALSLTVFLLKAAAAA
                     LKAFPRFNAVLDTDSEEILLKRFYHIGVAVDTDRGLIVPVIRDVDRKSITELARELRS
                     LAQKTREGKAGREDMTEGTFTITNVGALGGTGMSPIINYPQVAILGTARARLQPVVRG
                     DLDRYEIVPRLILPLVLTFDHRVLDGADAAQFLKFVVDVLEDPGRMLLQV"
     gene            675..2099
                     /gene="lpdA"
                     /locus_tag="PLM79_18250"
     CDS             675..2099
                     /gene="lpdA"
                     /locus_tag="PLM79_18250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOV88304.1"
                     /translation="MVMGEFTQDTELLVIGGGPGGYAAAFRAADLGMDVTMVDPAGRP
                     GGVCLFRGCIPSKTLLHVAELLRDARQAKEMGISFSEPEIRLESLRDWKNRVIEKLAS
                     GLAVVSKRRGVQVLQGRASFESSQMVRIVGGEVSHVRFKHAVIATGSRAAGLPGHPFR
                     KGSRVMGSTAALELGEIPQRLLVIGGGYVGMELGSVYASLGSRVTVVEWNERIMMGAD
                     GDLVQVLSSNLRELFQGIHLRTRVVSLEETERSVEVAFEGNIDPLEQSFDRVLVAVGR
                     LPNTSDLGIEKTGVALDSRGFIRVDERMRTADEKIYAVGDVAGGVMLAHKAMHEGKIA
                     VEVIAGRPSAFDFRAVPAVVYTDPQVAWCGLTEEEARRTGRPVRVSRFPWSASGRAVS
                     LGFLKGMTKILFDPETQRVLGMGIVGREAGEMISEGVLAVEMGALAEDLAMSIHPHPT
                     LSEGEEEAAGAFLGSSTHILPLKS"
     gene            2132..2863
                     /gene="lipB"
                     /locus_tag="PLM79_18255"
     CDS             2132..2863
                     /gene="lipB"
                     /locus_tag="PLM79_18255"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOV88305.1"
                     /translation="MENRTVCRYAWFGRVNYLDAWNLQKSLVSARAEGLISDRLLLLE
                     HPPTYTLGPRGDLSHLLEDAKTLEELGACLFQVDRGGDITFHGPGQLVGYPILSLQHR
                     PGGPSRYMRDLEAVILRTLETFGISGCRIPGYTGVWVDGEKVAAIGVKINARRIAGHG
                     FALNVSTDLSFFDRIVPCGIHGRKATSMEKILKGRVSIEEVRREIVGNFGEVFQLSME
                     PDTTESALYSVVCGPENEGSHADRP"
     gene            2847..>3353
                     /locus_tag="PLM79_18260"
     CDS             2847..>3353
                     /locus_tag="PLM79_18260"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOV88306.1"
                     /translation="MPTVHDFRDDNPPLRKPPWIRSRLPAGEAYHRVRELMSRQGLHT
                     VCREAACPNLGECWSSGTATFLILGDVCTRNCAFCAVKTGNPGGVPVDPSEPLAVAEA
                     VAAMELRHAVITSVTRDDLPDGGAQVFAATIQAVRERVPRCTVEVLIPDFSGDSEALD
                     VVLDAHPEV"
BASE COUNT          611 a         1045 c         1112 g          585 t
ORIGIN      
        1 cacgtcagcc acgaggaaac ggcggacatc accgaactgg acgcccttcg ccggaggcac
       61 aagggggaaa tcgaggccgc gggaggggcc ctcagcctca cggtcttcct gctcaaggcc
      121 gcagcggcgg ccctcaaggc ttttccccgg ttcaacgcgg tcctggatac ggattcggag
      181 gaaatccttc tcaaacgctt ctaccacatc ggagtggccg tggacacgga ccggggcctg
      241 atcgtgcccg tcatccggga tgtggatcgc aagagcatca ccgagctcgc ccgtgagctt
      301 cggtccctgg ctcaaaagac ccgggaaggc aaagcaggcc gcgaagacat gaccgagggc
      361 actttcacca tcaccaatgt gggagccctg ggaggaacgg ggatgagtcc catcatcaat
      421 taccctcagg tggccatcct cggtacggcc cgggcccggt tgcagcccgt ggtccggggg
      481 gacctcgacc ggtacgagat tgtcccccgg ttgattcttc cgctggtcct gacctttgac
      541 caccgggtgc tcgacggcgc cgacgccgcc caatttctga aattcgtggt ggacgttctc
      601 gaagacccgg gccggatgct cctgcaggtt tgaagcaccc gaggagaccg taggccccag
      661 gaggcaaatc gcacatggtg atgggggaat tcacccaaga cacggaactt ctcgtcatcg
      721 gaggaggacc gggaggctac gccgccgcat ttcgagccgc cgacctgggc atggacgtga
      781 ccatggtgga ccccgccggc cgcccgggag gcgtctgtct cttccgaggc tgcattccgt
      841 ccaagaccct cctccacgtg gcggaactgc ttcgtgacgc ccggcaagcc aaggaaatgg
      901 ggatctcttt ttcggagccg gaaatccggc tggaaagtct tcgagactgg aagaaccggg
      961 tcattgaaaa gctggcctcg ggactggcgg tcgtgagcaa gaggcgcggg gtccaggtcc
     1021 tccaagggcg ggcatctttt gaatcctccc aaatggtgcg gattgtcggg ggcgaagtca
     1081 gccacgtgcg tttcaaacac gcggtcatcg ccacggggtc ccgggccgcc gggcttcccg
     1141 gccacccgtt ccgcaaagga agccgcgtga tgggctctac ggccgccctc gaactgggag
     1201 agatccctca gcggctcctg gtgatcggcg gaggatatgt gggcatggaa ctggggtccg
     1261 tgtacgcgtc cctgggaagc cgggtgaccg tggtggaatg gaacgaacga atcatgatgg
     1321 gggccgacgg ggacctggtc caggtcctgt cgtcgaacct ccgggaactt ttccagggca
     1381 ttcatctccg gacccgggtg gtgtccctcg aggaaacgga gcgctcggtg gaggtggcct
     1441 tcgaaggcaa catcgatccc ctcgagcaga gtttcgatcg cgtgctggtg gccgtcggcc
     1501 gtcttcccaa cacctcggac ctggggatcg aaaagaccgg ggtcgccctg gattcccggg
     1561 gattcattcg ggtggacgaa cggatgcgca cggccgacga gaagatttat gccgtagggg
     1621 acgtggcggg aggcgtcatg ctggcccaca aggccatgca cgagggtaag atcgccgtcg
     1681 aggtcatcgc gggccggcct tcggcctttg actttcgagc cgttccggcg gtggtctaca
     1741 cggaccccca ggtggcctgg tgcggcctta ccgaggagga ggcccgcagg accggccgac
     1801 ccgtccgggt ttcacggttc ccgtggtccg cctcggggcg ggccgtttcc ctgggctttc
     1861 tcaagggcat gaccaagatc ctcttcgacc ccgagaccca gcgggtgctg ggcatgggga
     1921 tcgtgggacg ggaggccggg gagatgatct ccgagggagt gctggccgtc gagatggggg
     1981 ccctggccga agacctggcc atgagcatcc acccccatcc gactctttcc gaaggggagg
     2041 aagaggccgc gggggccttc ctgggaagct ccacccacat tcttccccta aaatcctagg
     2101 tcccgagcct ggagcagacg gggagagtcc catggaaaac cgcaccgtgt gccgatacgc
     2161 ctggttcgga cgggtgaatt acctggatgc ttggaatctc cagaaatccc tggtgagcgc
     2221 ccgggccgaa ggcctgattt ccgaccggct ccttctcctg gaacaccccc ccacctatac
     2281 cctgggcccc cggggagacc tctcccacct gctcgaggat gccaaaacac tggaagagct
     2341 gggtgcctgc ctgttccagg tcgatcgcgg gggagacatc acctttcacg ggcccggcca
     2401 gctggtggga taccccatcc tgagcctcca acacaggccg ggaggaccca gccggtacat
     2461 gagagacctc gaagccgtga tccttcggac cctcgagact ttcggcattt cgggctgccg
     2521 cattcccggc tacacgggag tctgggtgga cggagaaaag gtggccgcca taggggtgaa
     2581 aatcaacgcc cgcaggattg ccggccacgg ctttgccctg aacgtttcca cggacctgag
     2641 ttttttcgat cggatcgttc cctgcggcat ccacggcagg aaggccacca gcatggaaaa
     2701 gatcctgaaa ggccgggtgt ccatcgagga ggtccgccgg gaaatcgtcg gcaactttgg
     2761 ggaggttttc caactgtcca tggagcccga cacaaccgag tccgccctgt actcggttgt
     2821 ctgcggcccg gaaaacgagg ggtcccatgc cgaccgtcca tgattttcga gacgacaatc
     2881 ccccgttgcg caagccgccc tggattcgat cccgcctgcc cgcgggggaa gcctatcacc
     2941 gggtccggga actgatgagc cgtcaagggt tgcacacggt ttgccgagaa gccgcctgtc
     3001 ccaacctggg cgagtgctgg agctccggga cggccacctt cctgatcctg ggggacgtgt
     3061 gcacgagaaa ctgcgcattt tgcgccgtca agacgggaaa ccccgggggc gtcccggtcg
     3121 atccctcgga acctctggcc gtggcggaag cggtggcggc gatggaactg cgccatgcgg
     3181 tgatcacctc cgtcacccgg gacgacctgc ccgacggggg cgcccaggtt tttgccgcga
     3241 ccatccaggc cgtccgggaa cgggtacccc gatgcaccgt cgaggtgttg atcccggact
     3301 tttcagggga ttcggaagcc ctggacgtgg tgctggatgc ccatcccgag gtc
//
LOCUS       DAOOVC010000273         3336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15416_length_3336_cov_5.872905, whole genome shotgun sequence.
ACCESSION   DAOOVC010000273 DAOOVC010000000
VERSION     DAOOVC010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15416_length_3336_cov_5.872905"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PLM79_18265"
     CDS             <1..703
                     /locus_tag="PLM79_18265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOV88307.1"
                     /translation="KFALTARLADRVVAGNAYLKEQTLPHNPNVQVLPTPLDMSRYTP
                     RSSLQSPKKACTLGWIGSRPTLRHLEVLSPVFLELGRRHPDLRLKIVADAFPDGPTLP
                     VIAKRWDFNEEISDLHSFDIGLMPLNDDVWSRGKCGFKLLQYMAVGLPAVCSPIGLNR
                     EIVEHGRNGLWADSPREWIDRLEELLEDPSRRTSLGSRARETVLERYSLAVNAPLFAR
                     ILAQVTDSPLFGPAG"
     gene            707..>3336
                     /locus_tag="PLM79_18270"
     CDS             707..>3336
                     /locus_tag="PLM79_18270"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HOV88308.1"
                     /translation="MKIGLVIKQVSLSVGGAERFALNFAGTMAREGHDVHILASSWDR
                     EIPGVRYHRVAARRKPGWLNILQFHGNCRKILDRQTFDIVYGLTQFHPQDVFRMGGGI
                     YAQWLRVRYPNPFSRWAHYLIRPTFLVNLYMERKIFQPGNFLRVVGNSEKCKEELIRF
                     YRVRPEQVDVVYNGVDHSVFHPGIRDKLRMSTRADLGVREDQKLLLYAAASNWKRKGL
                     DTLLKALIPLEGFLLIVVGKDKEPRYRKKVLGWGIESKVRFLGFQKDIEAFYAAADFL
                     VLPTLYDPFSNVCLEAMACGLPVITSTGNGASEILRHGVNGFVLQDAKDPLELRRVLE
                     TILLEDSWEDMGKAAYRTSLDFTLEKNVADTLAVCRRALREKSGFKVLSLEGIRWHHV
                     SPGGPPRLKQWSEGTLEVEVVKSSPMRQTVRHAPGVILKEGLYSGTRAILRTVGGAKA
                     CREGSTLLKLARRGIPVPEVLAYGTQKRSGILERDILLTREVPGAQNLNTYVKRDYPG
                     LPFRTKRELIRKFAGFIRELHDAGVDHRDLHIGNILLRDPGSDNPFVLLDADRIRLKK
                     QGLSLRERVRNLALLLLNFWTLSSRAERLRFARDYGLDTKDLQVRDLLDRLEAITLDL
                     AGKVWDGKTRRCLSSNARFRKHRQDAFRILRVNRSEAEAVLKELLPDPDRLFGKGTTV
                     KEGRTTKSCRVEVGDRAYFLKRYNRKGWFYSLRNALRRCRAVRSWLAAWNLLERGVPV
                     PEPILCLEERKFRLLKRSYLVSEYIGNARGLPAVWPETDAPARRTLLARLAVLLGRMH
                     RAGVIHGDLKWSNILLPAGPGEKPPVLCDTDGTRVLRSRDPAPCRKDLDRFLRDLDRQ
                     GGPPEERRFFESVWTRWSGA"
BASE COUNT          653 a         1027 c         1007 g          649 t
ORIGIN      
        1 gaagttcgcc ctcacggcca gactcgccga ccgggtcgtt gcaggcaacg cctatttgaa
       61 ggaacagacc ttgccccaca accccaacgt tcaagttctc ccgactcccc tggacatgtc
      121 ccgctacacc ccacggtctt ccctccaatc ccccaagaag gcctgcaccc tgggctggat
      181 cggaagccga cccaccctgc gacacctgga agtcctgtcc cccgttttcc tcgagttggg
      241 aagacgacac ccggatctga ggctcaagat cgtcgccgat gcgtttccgg acgggcccac
      301 cctgccggtg attgccaagc ggtgggattt caacgaggag atctcggatc ttcattcttt
      361 cgacatcgga ctcatgcccc tcaacgacga cgtctggtcc cggggaaagt gcggcttcaa
      421 gcttctccag tacatggccg tgggactccc ggccgtttgt tcccccatcg gcttgaaccg
      481 ggaaattgtg gaacacggga gaaacggcct gtgggccgat tctccccggg aatggatcga
      541 ccggttggag gaactcctgg aggacccttc gaggcggacc tcactgggaa gccgggcccg
      601 ggaaaccgtc ctggaaagat actcccttgc ggtgaacgct cccctctttg cccgcatcct
      661 ggcccaggtg acggattccc ccctctttgg accggccggg tgatccatga aaatcgggct
      721 ggtcatcaag caggtttccc tgtccgtagg cggggccgag cgattcgccc tgaattttgc
      781 cgggaccatg gcccgggagg ggcatgacgt gcacatcctg gccagctcct gggaccggga
      841 aattcccggt gtgcgctacc accgggtggc ggcccgaaga aaacccggat ggctcaacat
      901 cttgcagttc cacggaaatt gtcgaaagat cctggaccgg caaaccttcg acatcgtcta
      961 cgggctcacc cagttccatc cccaggacgt cttccgcatg gggggcggca tctacgctca
     1021 gtggcttcgc gtgcgctatc cgaacccgtt ctcgcgatgg gctcattacc tcatacgccc
     1081 aaccttcctg gtcaatctgt acatggaacg caagattttt cagccgggga atttcctccg
     1141 ggtggtgggc aactccgaga agtgcaaaga agagctcatt cgcttttacc gcgttcgtcc
     1201 ggaacaggtg gacgtggtct acaacggggt ggaccattcc gtgttccacc cggggattcg
     1261 agacaagctc cgaatgagca cccgggcgga cctgggcgtg cgggaagatc aaaagctgct
     1321 tctttatgcc gccgcctcca actggaagcg aaagggcctg gacaccttgc tgaaggcctt
     1381 gattccattg gaaggctttc tcttgatcgt ggtgggcaag gacaaggaac cacgataccg
     1441 gaagaaggtc ctgggatggg gaatcgaaag caaggtccgc ttcctggggt ttcagaagga
     1501 catcgaagcg ttctatgctg cggcggactt cctggtcctc cccactcttt acgacccctt
     1561 ctccaacgtt tgcctggaag ccatggcctg cgggctcccc gtcattacct cgacgggcaa
     1621 cggggcttcc gaaatccttc ggcacggggt gaacggattc gtgcttcaag acgccaagga
     1681 tcccctcgaa ctccggcggg tcctcgagac gatccttctc gaggattcat gggaggacat
     1741 ggggaaggcc gcctaccgca cctccctcga tttcaccctc gagaagaacg tggcggatac
     1801 cctggcggta tgccgccggg cgctgagaga aaagtccgga ttcaaggttc tttccctgga
     1861 agggatccgg tggcaccatg tttcccccgg gggacccccc cggctaaaac aatggagcga
     1921 ggggaccctc gaggtcgagg tggtaaaatc ctctcccatg agacaaacgg tgcgtcatgc
     1981 cccgggggtc atcctcaagg aagggctcta ttcgggaacc agggcgatct tgcgaaccgt
     2041 ggggggagcc aaggcgtgcc gggagggaag cacccttctg aaactggccc gccggggaat
     2101 tccggtcccc gaggtcctgg cctacgggac ccaaaagcgc tcggggattc tggaacgcga
     2161 tattctgctg acccgggaag taccgggagc ccaaaatctg aacacctatg tgaagcggga
     2221 ttacccgggg cttcccttcc gcacaaaacg ggagctgatc cgaaagtttg ccgggtttat
     2281 ccgggaactc cacgatgcgg gcgtggacca ccgagacctt cacatcggga atattctgct
     2341 gcgggacccg gggtcggaca accctttcgt cctgctggac gccgaccgta ttcgcctcaa
     2401 aaagcaggga ctctctttgc gagaacgggt ccggaacctg gcccttcttc tcctcaactt
     2461 ctggaccctg agcagccggg cagagcggtt gcggttcgcc cgagattatg ggttggacac
     2521 aaaggacctg caggttcggg accttttgga ccgactcgag gcaatcaccc tcgatctcgc
     2581 cgggaaagtc tgggacggaa agactcggcg atgcctgtct tccaatgccc ggttccgaaa
     2641 gcaccgacag gatgcctttc gaattctgcg ggtgaatcga tcggaagcgg aagcggtgtt
     2701 gaaggagctc cttcccgacc ccgatcggct tttcggtaaa ggaacgacgg tcaaggaggg
     2761 ccggactaca aaatcctgcc gggtggaggt gggagaccgg gcgtattttc tcaagcggta
     2821 caaccgcaaa ggatggttct acagtcttcg caacgccctt cgtcggtgcc gggcggttcg
     2881 atcctggctt gcggcgtgga acctcctgga aagaggcgtc ccggtacccg aacccatcct
     2941 ttgcctcgag gaacgcaagt ttcgcttgtt gaaacgatcc tacctcgttt cggaatacat
     3001 cggaaacgcc cgaggtttgc cggctgtttg gccggagacg gatgcgccgg cccggagaac
     3061 cctcctggcc cgcctggccg tcctcctggg ccgcatgcac cgggcggggg tgattcacgg
     3121 agacctcaag tggagcaaca tcctactccc cgcaggcccc ggggagaagc ccccggtgct
     3181 gtgcgacacc gacggaactc gagtccttcg ttctcgggac ccggctccat gccgcaaaga
     3241 cctggatcgc tttctccggg acctggaccg gcagggaggc ccccccgagg aacgtcggtt
     3301 tttcgagtcc gtttggactc gatggtcggg ggctca
//
LOCUS       DAOOVC010000274         3313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15546_length_3313_cov_6.679251, whole genome shotgun sequence.
ACCESSION   DAOOVC010000274 DAOOVC010000000
VERSION     DAOOVC010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15546_length_3313_cov_6.679251"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLM79_18275"
     CDS             complement(<1..537)
                     /locus_tag="PLM79_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOV88309.1"
                     /translation="MLQETTIPKVPSDKERQLLVCRGTGCESQKAKLLYNTLVDAVYE
                     AGLADRYEVKFTGCHGLCQMGPTVMITPEGTFYCTVTPDDVPEIIETDLKRGGKVERL
                     LFFDPKSKTHVFSYKDMKFFGPQRRIVLRNCGFINPEVIDDYIAVGGYEAIRKVFGMS
                     RMDVINEVKKSGLRGRGGG"
     gene            complement(512..979)
                     /gene="nuoE"
                     /locus_tag="PLM79_18280"
     CDS             complement(512..979)
                     /gene="nuoE"
                     /locus_tag="PLM79_18280"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOV88310.1"
                     /translation="MKSGEKLQQIFARHDPRRDALIPILQDIQAEYGYLPPEAMEAAA
                     GHCRMHPVEVFGVSTFYAQFKFDPVGRNTLMVCQGTACHVMGGARILEEVQSQLGIRP
                     GQTTPDGAFTLETVACIGACALAPAMVVNKDTHGRMKPEKVMEILHAARNDNS"
     gene            complement(1105..2730)
                     /locus_tag="PLM79_18285"
     CDS             complement(1105..2730)
                     /locus_tag="PLM79_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HOV88311.1"
                     /translation="MDIGSYSYEEYLRLVESFHNYAAPGVLLGGFMVDLALKNLPGGI
                     LFEAVSETPKCLPDAIQLLTPCTTGNGWLKVLNLGRYALSLYDKYTGQGVRVALDAAR
                     LDARPHIRAWLFKTRPKSEQDSQRLLAEIRSAGHDPYKVHPVRIPVDRFQTKRKGKIG
                     LCPLCEEAYPLADGPICRGCRGEAPYETSEDFREERRPPLKALPLEQAVGKKSLHDLT
                     MIIPGKSKGPAVFHDQPITVGDLCRLQTMGRRRIYVREENPPHPDWVHENEAAVAFAE
                     GMVGEGVGFHGPPQEGKVNFAAQRNGLFWVDERRLEQFNLVPGVMCACRKGYTPVSEG
                     RQVAGTRAIPLFLPKDHFLKAMAVLSQGPLFRVLPLRKARVGILVTGSEVFDGLVEDR
                     FIPIIGAKVRHYDCEVIQAQVVPDEREAIRKGVLDLVRSGADLIVTTAGLSVDPDDVT
                     RQGLLDAGVTDMTYGAPILPGAMTLLARLGPVQVIGVPACALYFKTTSFDLLLPRLLA
                     GLTITREDLARLGHGAFCIECKSCTFPKCPFGT"
BASE COUNT          673 a          926 c         1019 g          695 t
ORIGIN      
        1 tccgcctccc cggcctcgaa gcccggattt tttcacctca ttgatgacgt ccatccggga
       61 catgccgaag acctttcgaa tggcttcata gccgcccacg gcgatgtaat cgtcgatgac
      121 ctcggggttg atgaacccgc agtttcgaag gacgatccgg cgttgaggac cgaagaactt
      181 catgtccttg tagctgaaca catgggtttt ggacttggga tcgaagaaga ggaggcgctc
      241 taccttcccg ccccttttga ggtcggtttc gatgatttcg ggaacgtcgt cgggagtgac
      301 ggtgcagtaa aaggtccctt caggggtgat catcaccgtg ggccccattt ggcacagtcc
      361 gtgacacccc gtgaacttga cctcgtaacg atccgccaga ccggcctcat acactgcgtc
      421 caccagggtg ttgtagagca acttggcctt ctgggattcg caaccggtcc cgcggcaaac
      481 gagcagctga cgttccttgt cggagggtac cttaggaatt gtcgtttctt gcagcatgca
      541 gaatctccat cactttctcg ggtttcattc ggccgtgggt atccttgttg acaaccatgg
      601 ccggtgccag ggcgcaggcc ccaatgcagg ccacggtctc gagggtgaag gccccgtccg
      661 gggtggtttg gcccgggcgg atccccaact gggactggac ttcctccaga atacgggctc
      721 cccccatgac atggcatgcg gtcccttgac agaccatcag ggtgtttcga cccaccgggt
      781 cgaatttgaa ttgtgcatag aatgtggaca cgccgaagac ctccacggga tgcatccggc
      841 agtgaccggc agccgcctcc atggcttcgg ggggcaggta gccgtattcc gcctggatgt
      901 cctgtaggat ggggatgagc gcgtcccgcc tgggatcatg acgggcaaaa atctgctgca
      961 atttctctcc gcttttcata ctccctccac ctcagggttg gttggattgg gttgttggat
     1021 gaatccacaa ggaaacgaac cattgcctca gcaggggtgt gaaaacggtc ggatcccgga
     1081 ggacggcggt ccgcatttgc gccctcacgt gccaaagggg cacttgggaa aggtgcagga
     1141 tttacattcg atacaaaagg ctccgtgccc cagcctggcc aggtcttccc gggtgatggt
     1201 cagtcccgcc agtagccggg gcagcaaaag gtcgaaactg gtggttttga agtagagggc
     1261 acaggccggg accccgatga cttggacggg acccagtcgg gccagcagag tcatggctcc
     1321 gggcaatatc ggggctccgt aggtcatgtc ggtcaccccg gcatccagca ggccttgtcg
     1381 ggtcacgtcg tcggggtcca cggagagccc cgcagtggtt acgatgaggt cggctcccga
     1441 tcggacgaga tccaggacgc cctttcgaat ggcctcgcgc tcgtcgggca ctacctgagc
     1501 ctggatcacc tcacagtcgt aatgccgcac cttggctccg atgatgggga tgaagcggtc
     1561 ttccaccaag ccgtcaaaca cctcgctccc cgtcaccagg atgcccaccc gggccttgcg
     1621 caagggaagg acccggaaaa gcggtccttg ggaaagaacc gccatggcct tgaggaaatg
     1681 atccttgggg agaaacaaag gaatggcccg ggtaccggcg acttgcctgc cctcgcttac
     1741 cggggtgtag cccttgcggc aggcgcacat gacgccgggg accaggttga actgctccaa
     1801 cctgcgttcg tcgacccaaa agagcccgtt tcgttgggcc gcaaaattca cctttccttc
     1861 ctggggaggc ccgtgaaaac ccactccctc gccgaccatg ccttcggcga aggcgacggc
     1921 cgcttcgttc tcatggaccc agtcgggatg aggaggattt tcctcccgga cgtagattct
     1981 ccggcgcccc atggtttgca gccgacacaa gtctcccacg gtgatcggct gatcgtggaa
     2041 gacggcaggc cccttgcttt ttccggggat gatcatggtc aggtcatgga gggacttttt
     2101 gcccacggcc tgttcgaggg ggagggcctt gaggggaggg cgacgttctt cccggaaatc
     2161 ctccgaggtc tcgtaagggg cttctccccg gcagcctcgg catatgggtc cgtcggccag
     2221 gggataggct tcctcacaaa ggggacaaag cccgatcttc ccctttcgct tggtttggaa
     2281 acgatccacc ggaatccgaa cggggtggac tttgtaaggg tcgtgcccgg cgcttcggat
     2341 ttcggcaagg agccgttggc tgtcctgttc ggacttgggc cgggttttga aaagccatgc
     2401 tcggatgtgg ggccgggcat ccaggcgggc tgcgtccagg gctacccgaa cgccttggcc
     2461 cgtgtatttg tcgtagagac tgagggcata acgtccgagg ttgaggactt tcaaccaccc
     2521 gtttccggtg gtgcaggggg taagaagctg gatggcgtcg ggcaggcact tgggggtttc
     2581 ggagaccgcc tcgaaaagga tccccccggg aaggttcttc agggccaagt ccaccatgaa
     2641 accgccgaga aggacgcccg gcgcggcgta attgtggaac gattcaacaa ggcgcaggta
     2701 ttcctcgtac gagtaggatc cgatatccat gacgatcgct ttctgagccg caatgagaat
     2761 ggagaggagc tggagcttcg cattttttca cggttggatt cgcctttgcc ggtgcgttgg
     2821 ttgggacaag gcatagcgcc atttttatac taacagcctc ggaagtcgtc aagggaaaag
     2881 gctcttctct atcttgttag aattaacata atcaacaact tagccttgtg ttgcccaaaa
     2941 catactttga caagaccaaa aaaagtatga tagcaaggaa cgaaatcgcg gacgggaggc
     3001 aaccggcaag aggggcccgt aaatgtttcc ccatcttttc ggcagatctc ctccccccgg
     3061 gggcgacggg ccccggtcaa cggaggggac gccgaaagaa gagatcccgg gatttcaacg
     3121 ggtgtgcggc tggtgtgatt gtccttcgtt tgattccggc aattatgact cattttggct
     3181 tgcagcggga agcatcatgt ggatcatcaa ctttatctta aggaggacgg gaaatgagca
     3241 acggcaacac gataatcatt gacgcactca tgcccaagga catggccgcc aaggcggaaa
     3301 acatcggggt ggc
//
LOCUS       DAOOVC010000275         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15721_length_3288_cov_7.503866, whole genome shotgun sequence.
ACCESSION   DAOOVC010000275 DAOOVC010000000
VERSION     DAOOVC010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15721_length_3288_cov_7.503866"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLM79_18290"
     CDS             <1..260
                     /locus_tag="PLM79_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV88312.1"
                     /translation="VLSTIRYFRDEYEAHILKKECPARVCRHLIKFRVNEEKCTKCGS
                     CFKACPVEAIRWEKKQPAFIDTEKCMKCRSCILACRFHAID"
     gene            332..3049
                     /gene="fdhF"
                     /locus_tag="PLM79_18295"
     CDS             332..3049
                     /gene="fdhF"
                     /locus_tag="PLM79_18295"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1412..1414,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOV88313.1"
                     /translation="MNKDIITLTVNGKDFKGKTVQGKRGQTVLEVFRDNGIQVPTLCY
                     HPKMPPYGGCRLCIVEIENMRGLPPSCTTPAADGMVIHTHTERVVQVRKTVLELLLAY
                     GDHNCLLCEQTGDCELQNLVYEHGIDRIRIKSDFKPRPKDDSNSMIVRDQNKCVLCGR
                     CVRGCLEVQVNGVIEVALRGSDSFITTFDHTPLKDSNCLFCGECVSVCPTGALTYKQA
                     RFKGRSWELKKVPTTCTYCGVGCQMELNVKDNTIIKVTSTDEIPGPNKGSLCVKGRFG
                     YDFVNHPDRLTTPLIRERGGFREASWEEALDLVANSFSKIKQENGPDSLGFFTSARCT
                     NEENYLMNKLARAVVGTNNIDHCARLUHSSTVAGLAAAFGSGAMTNSIEEFEKAEVLL
                     ITGTNTTEMHPVISSYFKRAVRNKKTKLIVVDPRKIDLVDHAEIWLRQKPGSDVAWLN
                     GMMHVIIREDLYDKQFVEERTEGFEALKKVVEAYTPEKVQEISGIPAEDLVAAARLYA
                     KASAASIAYAMGITQHINGTDAVKSVANLAMLCGNIGKEGTGVNPLRGQNNVQGACDM
                     GGLPNVFPGYQPVGSPEVRAKFEKAWGVEKLPEKVGKTIVEIVNGAIDGSIKGLYIVG
                     ENPMVSDPDLHHVEQGLNSLEFLVVQDIFLTETAKLADVVLPSFCFAEKEGTFTNTDR
                     RVQRVRKAVDAPGAAMDDWRIICGIATRLGYPMNYADAQEIFQEIRQVTPQYAGMSYE
                     RLDQGGLQWPCPTEEHPGTKILHTQTFSRGKGLFHAIDWIPPAEPTDDQYPFVLTTGR
                     VLYQYHTGSMSRRSSALSELYPECLVEINERDASALDINDGDSVKIESRRGHIVAKAR
                     VGDKTEVGTIFVPFHFAEAAANQLTIAALDPVAKIPEYKVCAVKVQRAN"
BASE COUNT          751 a          969 c          991 g          577 t
ORIGIN      
        1 ccgtgctttc caccatccgg tacttccggg acgagtacga agcccacatc ctcaagaagg
       61 agtgcccggc ccgggtctgc aggcatctga tcaagttccg ggtcaatgaa gaaaagtgca
      121 ccaagtgcgg cagctgcttc aaggcatgtc cggtggaggc cattcgttgg gaaaagaagc
      181 aacccgcatt catcgacacc gaaaaatgca tgaagtgccg gtcctgcatc ctggcctgcc
      241 ggttccacgc gatcgactga tcgcgcccgg acccccaggc acgaggattt gaacggatac
      301 ccgtattccc acaaccagcg agagaggatc catgaataag gacatcatta ccctgacagt
      361 caatgggaaa gatttcaagg ggaagacggt ccaggggaaa aggggacaga cggtgttgga
      421 agtcttccgg gacaacggaa tccaggtccc gaccctctgt taccacccca aaatgccgcc
      481 ttacggtggc tgccgccttt gtatcgtcga aattgaaaac atgcgcggac ttcccccctc
      541 ctgtaccacc ccggctgcgg acggaatggt cattcatacg catacggaac gagtggtcca
      601 ggttcgaaag acggtgttgg aactgctcct ggcttacggc gaccacaact gcctgctctg
      661 cgaacagacg ggagattgtg agctgcaaaa cctggtctac gagcacggga tcgaccgaat
      721 ccgcatcaag tcggatttca agccccggcc caaagacgac agcaattcca tgatcgttcg
      781 ggatcaaaac aagtgcgtgc tgtgcgggcg ctgcgtgaga ggctgtctcg aggtccaggt
      841 gaacggagtc atcgaagtgg cccttcgagg atccgactct ttcatcacca cctttgacca
      901 cacgcccctc aaggactcca actgcctgtt ctgcggagaa tgcgtctcgg tgtgccccac
      961 gggagccctc acctacaaac aggctcgctt caaggggcga tcctgggagc tgaaaaaggt
     1021 ccccaccacc tgtacctatt gcggggtggg ctgccaaatg gagctcaacg tcaaggacaa
     1081 caccatcatc aaggtgacgt ccacggacga aatccccggt cccaacaagg gaagcctgtg
     1141 cgtgaagggc cggttcggct acgacttcgt caaccatccg gaccgcctga cgactcctct
     1201 gatccgcgag agaggcggct tcagggaagc ttcctgggaa gaggccctgg acctggtggc
     1261 caacagcttt tccaagatca agcaggaaaa cggccccgac agtctgggct ttttcacctc
     1321 ggcaaggtgc accaacgaag agaactatct catgaacaag ctggcccggg cagtggtggg
     1381 caccaacaac atcgaccact gcgcacgcct ctgacactcc tctaccgtgg ccgggctggc
     1441 cgcggcgttt ggaagcggtg ccatgaccaa ttccatcgag gagttcgaga aggccgaggt
     1501 actgctgatc accggcacca acaccacgga aatgcatccg gtgatctcca gctatttcaa
     1561 gcgggccgtg cgaaataaga agaccaagct catcgtcgtg gaccccagga agatcgacct
     1621 ggtggaccat gccgagatct ggcttcggca gaagccgggc tccgatgtgg cctggctcaa
     1681 cgggatgatg cacgtcatca tccgtgagga tctctacgac aagcagtttg tggaggaacg
     1741 gaccgaaggg ttcgaggcgt tgaagaaggt ggttgaggct tataccccgg agaaggtcca
     1801 ggagatttcc ggcattcccg ccgaggacct cgtggccgcc gctcgacttt acgccaaggc
     1861 ctcggcggct tccatcgcct acgccatggg catcacccaa cacatcaacg ggacggacgc
     1921 cgtgaaatcc gttgccaacc tcgcgatgct ctgcggaaac atcggcaagg aaggaacggg
     1981 cgtcaacccg ctgcgcgggc aaaacaacgt gcagggggcc tgcgatatgg gcggccttcc
     2041 caatgtcttt ccgggatacc agcccgtagg atctcccgag gtgagggcga agttcgagaa
     2101 ggcgtggggc gtggagaaac tgccggagaa ggtggggaag accatcgtgg agatcgtcaa
     2161 cggcgccatc gacggctcca tcaagggact ctacatcgtg ggagaaaacc ccatggtgag
     2221 cgacccggat ctccaccacg tggagcaagg gctcaacagt ctcgagttcc tagtggtcca
     2281 ggacatcttc cttacggaaa cggcaaagtt ggcggacgtg gtgctgccct ccttctgctt
     2341 tgcggaaaag gaggggacct tcaccaacac cgaccggcgc gtgcagcggg tgcgcaaggc
     2401 ggtggacgct ccgggggcgg ccatggacga ctggaggatc atctgcggga tcgccacccg
     2461 cctgggatac cccatgaact acgcggacgc gcaggaaatc ttccaggaaa tccgccaggt
     2521 gacgcctcag tacgccggga tgagctacga gcgcctggac caaggcggac tccagtggcc
     2581 ttgccccacc gaggaacacc cgggaacgaa gatccttcat acccagacct tctctcgggg
     2641 aaagggactt ttccatgcca tcgattggat tccgcccgcg gagccgacgg atgaccagta
     2701 ccccttcgtt ctcaccacgg gccgggtgct ctatcagtac cataccggca gcatgagccg
     2761 tcgatcgagc gctctgagcg agctctatcc cgagtgtctt gtggagatca acgagcggga
     2821 tgcttccgcc ctggacatca acgatggaga ctctgtgaag atcgaatccc gccgcggcca
     2881 tatcgttgcc aaggcccgag tgggggataa gacggaagtg gggaccatct tcgtgccctt
     2941 ccactttgcc gaggccgcgg ccaaccagtt gaccatcgcg gccctggacc cggtggcgaa
     3001 gatccccgag tacaaggttt gcgccgtgaa ggtgcagagg gcaaactaag acgtagcccg
     3061 gagaactcat cgaggtctcc gggtaacctt tccctgccgt ttcgggctag ggaagagagg
     3121 aaatgttccc ttccggagaa agacggggcc cgggtccgtc cggaagctcg ttgaacggca
     3181 ggggaagaag ggagtgggag gagcgatcgt tcgccccccg attcatttca tctaggagga
     3241 cgggaaatga gcaacggcaa tgcgatatcc tttgacgcac tcatgccc
//
LOCUS       DAOOVC010000276         3264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15860_length_3264_cov_5.655033, whole genome shotgun sequence.
ACCESSION   DAOOVC010000276 DAOOVC010000000
VERSION     DAOOVC010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15860_length_3264_cov_5.655033"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..513
                     /locus_tag="PLM79_18300"
     CDS             352..513
                     /locus_tag="PLM79_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88314.1"
                     /translation="MKVIFIRGRNRQETRKKTLDFWFAHRDQFNCTMKDFLRKCSTDP
                     TGRIVMYRE"
     gene            766..975
                     /locus_tag="PLM79_18305"
     CDS             766..975
                     /locus_tag="PLM79_18305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88315.1"
                     /translation="MSGVTELLCVGLKRSVASMLPWDIPWYDPSHTVFFLALYGALGV
                     IGLGMATAAVMTYLKLTKGDQEGHH"
     gene            1450..2808
                     /locus_tag="PLM79_18310"
     CDS             1450..2808
                     /locus_tag="PLM79_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOV88316.1"
                     /translation="MDPKVFREYDIRGIVGKEIHDPDVVRLGKAFGTLMMEKGRERIV
                     VGRDCRLSSERYRDLLVEGLVSAGMDVADVGICPTPLFYFAIRHLDRAGGIMLTASHN
                     PPEYNGFKICDGYDTVFGSEIQRLRQKMEEGGFVSGNGRVGFNEIFEPYREFVLGNVR
                     LGRRLRVGVDAGNGTGGPVALPVLRGLGCEVMPLYCDMDGSFPNHHPDPSVLDHLNDL
                     IALVRRERLDVGLAYDGDCDRLGIVDHRGEVVYGDRLMILFARDILSKRPGSTIVAEV
                     KCSQTLFDDVQRRGGKAVMWKAGHSLIKAKMRELGAVLAGEVSGHLCFNDRYFGFDDG
                     IYASCRLLEVLARTGQTIPQLLEDVPRTFVTPEIRVGCPDEIKFQLVEKVRDHLRRAG
                     HSIIHVDGVRICLADGWGLLRASNTQPALVLRFEARTRQRLDEVRGLVEQVLEISRKE
                     IV"
     gene            2955..>3264
                     /locus_tag="PLM79_18315"
     CDS             2955..>3264
                     /locus_tag="PLM79_18315"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOV88317.1"
                     /translation="MGETTEKKAAVVTGVAGFVGSHLASRLLELGFGVVGVDNLFSGK
                     LENIRSMQGRPGFSFYRRSVTEPGLLPQLKAEHPHLNYCFHLAAIVSVPYSLDHPQET
                     M"
BASE COUNT          639 a          927 c         1020 g          678 t
ORIGIN      
        1 acatcaaacc tgaaaataat gcacgcatat ttgttgcttt ttcaacaccc tgctaagccg
       61 tggacgggag gaagaaataa ttcttgactt ttgagcaaac cggggtcaaa agggggtgcg
      121 aggtgcaaga agacctcggt gtgctgaacc ccagtagaga agcaaaatgg aagattcgga
      181 cagtagcata cggtgttctt ttagaaaacc ggcaccaagg gatttcaggc tcgggtatgc
      241 acccggggtg cgggggattc ccccttcgcc ctgatccatg ccaatacggt tgctcaaagc
      301 ccgagcctcg gctcgggctt ttttttgccg atggaaacga gaggtgacag gatgaaggtc
      361 attttcattc gcggacgcaa ccgtcaggaa accaggaaga agaccttgga cttttggttc
      421 gcccaccggg atcagttcaa ctgcaccatg aaggacttcc tcaggaagtg ctcgacggat
      481 cccacgggcc ggatcgtcat gtacagggag tgagaagcca tggaagctca aatacccggc
      541 tggctgcggc cggcatcccc aggctgaaag gcaacccctt tcagcctttt ttgttgcttc
      601 cttccggcat acccaacctt tatgaatctc tttgcgaagt gcccattgcc ctcccacttt
      661 tcgaattgac cccggagcaa acttttggta gaggggacag acagttgaca aagatggttt
      721 ttccaaaccc gatcggcgca ggagatcatg taagggggtg gttgtatgtc aggagtaacc
      781 gagcttcttt gtgtgggtct gaaacgcagc gtcgccagca tgcttccttg ggacatcccg
      841 tggtatgacc cgagtcacac cgtcttcttc ctggctcttt acggcgccct gggagtgatc
      901 ggcctgggca tggcgaccgc ggccgtcatg acctacctga agttgaccaa aggcgaccag
      961 gaaggccacc actaggaacg agggcccctc gatcgttttc ttcgggaatc ccgaatcgag
     1021 tgtcggcgcc tgcggcctta tgccgggaaa tccgcgaggt ccaaggccgg gagccgccgc
     1081 gacctgcgca actccccgac gcccgaaccg gggtgccgtt acggtgccgg ggaccctcgg
     1141 agggaacccc gcacctttca gcctccattc ccacctccgg attgccgaat tttcgtttcc
     1201 agaccacggc agaccaggga aacccgggcc gcgaaaaggc gagggccttg cgctctctcc
     1261 tgcgaacccc tcttgtggag gaccgggccc tgcggaggag aaacccttcg gctcccccgg
     1321 gcctcccccc gacgggaagc cctgttgctt cgtgttttcg catttccccc tccgtgcagg
     1381 ggggcagtcg tccggaaacc attgccgggc atcttgccgc ccggaccttc aatcattgcg
     1441 agggaaccgg tggaccctaa agtctttcgg gaatacgaca ttcggggaat tgtcggaaag
     1501 gaaattcacg accccgatgt ggttcgtctg gggaaggcct tcgggaccct catgatggaa
     1561 aaaggtcgag agcgaattgt ggtgggacgg gactgtcggt tgagttccga gcgttaccgg
     1621 gacctcctgg tggagggatt ggtttctgcg ggtatggacg tggcggatgt ggggatctgc
     1681 cccacgccgc tcttttattt tgccatccgg cacctggacc gcgcgggcgg gatcatgctc
     1741 acggccagcc acaacccccc ggaatataac ggattcaaga tctgcgacgg atacgatacc
     1801 gttttcggct ccgagatcca aagactgcgg caaaagatgg aggagggcgg gttcgtctcc
     1861 gggaacggcc gggtgggatt taatgagatt ttcgaaccct accgggagtt tgtgttgggg
     1921 aatgtccgcc tcggcagaag gcttcgagtg ggggtggacg ccggcaacgg tacgggaggt
     1981 cccgtggcgc tccccgttct tcgcggactg ggctgcgagg tgatgcccct ctattgcgac
     2041 atggacggct ccttccccaa tcaccacccg gacccgtcgg tgctcgatca tctcaacgat
     2101 ctcatcgctt tggtgcgacg ggaacgcctg gacgtaggcc ttgcctacga cggggactgt
     2161 gatcgcctgg ggatcgtgga tcaccgggga gaagtggtgt acggagatcg gttgatgatc
     2221 ctgtttgccc gggatatcct gtcaaagagg cccggctcga ccatcgttgc cgaggtgaag
     2281 tgctcccaga ccctcttcga cgacgtccaa aggaggggag ggaaggccgt gatgtggaag
     2341 gcgggccact ccctgatcaa agccaagatg cgggaactgg gcgcggtgct ggcgggggaa
     2401 gtgagcggcc acctgtgttt caatgatcgg tacttcggct ttgacgacgg catctacgct
     2461 tcctgtcgtc tcctggaggt cctggcccgg acggggcaaa ccattccgca actgctcgaa
     2521 gatgtccccc ggaccttcgt cacccccgaa attcgagtgg gctgtcccga cgagatcaaa
     2581 tttcaactgg tggagaaggt ccgagatcac ctgcgccggg ccgggcattc gatcatccat
     2641 gtggacgggg tgcgcatctg ccttgccgac gggtggggct tgcttcgggc gtccaacact
     2701 cagcccgccc tggtgctccg cttcgaggct cggacgcggc aaaggctcga tgaggtccgg
     2761 ggcctggtgg aacaggtcct tgaaatctcc cggaaggaaa tcgtctaggc ccccccgttc
     2821 tcgaaatgct tccgcagcat cccgggtcct ttgacggctt cctccccttg tgggattctc
     2881 cggcgtgtgt caacgaccgg agtgtgcgca tgggccaccg ggtgggagtc taccggagga
     2941 tggatcggag agacatgggg gaaacaactg agaagaaggc ggcagtggtc acgggagttg
     3001 cggggtttgt gggcagtcat ctggcttctc ggcttctgga attgggattc ggggtcgtgg
     3061 gggtggacaa cctcttctcg gggaagctgg aaaacatccg gtccatgcag ggtcgtcccg
     3121 ggttcagctt ctaccggagg tccgtgacgg aacccggact cttgccccag cttaaggccg
     3181 aacatcccca tttgaactac tgtttccact tggccgccat cgtcagcgtg ccctactccc
     3241 tggaccatcc ccaagaaacc atgg
//
LOCUS       DAOOVC010000277         3245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_15980_length_3245_cov_4.439498, whole genome shotgun sequence.
ACCESSION   DAOOVC010000277 DAOOVC010000000
VERSION     DAOOVC010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15980_length_3245_cov_4.439498"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /locus_tag="PLM79_18320"
     CDS             <1..1188
                     /locus_tag="PLM79_18320"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOV88318.1"
                     /translation="VAQKALSCVYRTGQRFGAAYLTDVLLGKEDERIRRFGHDKVSTF
                     GIGTELSEGEWKSVFRQLVAAGLLSVDIEGKGGFRLASPSRPVLRGEHRFELRKDPVP
                     AKSKKTSRPKPERGSVPSDSISGELWEQLRSLRLEIAREQAIAPFLVFHDTTLKEMVK
                     HLPRSLEEMRGISGVGQKKLELYGDRFLSIIRKHGEGTALPDIETGSDAVKRGNCCQG
                     TGEVESTSTRANSQQQKAEVIRLLKEGKLSSGEIAERVGISPPTVWAYKAHLTIGTYG
                     DDKESKTGRNADHESAGATGVHDFHSDAPIDQFQEGDIPLADLRELIINKPLKMPTDS
                     ITDQRLLEIRKKYRRAYEPWSNEEDEWLLRSHKEKQSNAALAKTLERQPSAIRSRIRK
                     FTG"
     gene            complement(1818..>3245)
                     /locus_tag="PLM79_18325"
     CDS             complement(1818..>3245)
                     /locus_tag="PLM79_18325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOV88319.1"
                     /translation="NRSECRAAVESAHRLMLGGNENPHERWKFAEEKRRRDVDRDHPR
                     WRGKNPELAGFIEAYEAELRRRDLIDFDDMPLLALRIIREHEWVRRSLRAKFPVLFVD
                     EYQDLGHALHELVLLLCFNVGVRLFAVGDPDQSIYSFLGANPKLLENLSSRAEVRSIR
                     LRFNYRCGTSIIHASMAALGEERDYCCPDDAEAGTIEFHGIKGELNAQADFVFASLLP
                     SIRKCGTTMEQIGVLYRWATQGDEVAAKAMEYGIPIVRADNNALIKRSSRLSRFVEAC
                     ANWVAGGWKEANPPFRRLAADAATLVLGPGATKEEQLQIQAQLICFLRSTIGCNMSTH
                     EWLSAFRREALLPWRKRARTIVEDWDAIDLMIERTDPTKEAGNHLPLSHFGGRIEGSG
                     RLNLSTLHSAKGREFDAVILFAMNRDVIPNSFERRSEAQLREARRQFYVGVTRARRHL
                     YLVCNRESYSPFVAELYRRTQQPYT"
BASE COUNT          793 a          881 c          874 g          697 t
ORIGIN      
        1 gtggcacaga aggcgctctc ttgcgtttac cgaaccgggc agcgctttgg agcagcctat
       61 ctcacggatg ttctccttgg aaaagaggac gagcggatcc gccgcttcgg tcacgacaag
      121 gtttcgactt tcggcattgg aacggagcta tccgaggggg agtggaagtc cgtatttcgc
      181 cagctggttg ccgccggtct cctgagtgtc gatatcgaag gaaagggagg attcaggctc
      241 gcatcaccca gccggcccgt cttgagaggc gagcacagat ttgagcttcg aaaggacccg
      301 gtaccagcca aaagcaagaa aacctcaaga ccgaagcccg agcggggaag tgtcccatcg
      361 gattccatct ccggagagct gtgggagcag ctgcgctcat tgcgccttga gattgccaga
      421 gaacaggcta ttgcaccttt ccttgtcttt catgacacca cgttaaagga aatggtgaaa
      481 catcttcccc ggtccctaga ggagatgcgg ggaatttccg gtgttgggca aaaaaagctc
      541 gaactctatg gcgatcggtt tctctcaatc atccgaaaac acggggaagg gactgctcta
      601 ccggacatcg aaactggctc agacgctgta aagagaggaa actgttgtca ggggactggc
      661 gaagtcgagt cgacctcgac gcgtgcgaat tcacagcagc agaaagccga agtgattcgt
      721 ctcctcaaag aggggaagtt gagttcgggt gagattgccg aacgtgtcgg gatatccccg
      781 cccactgtgt gggcctacaa ggcccatctc accatcggaa cttacgggga cgacaaggaa
      841 agcaagaccg gccgaaatgc cgatcatgaa tccgccggcg ctacaggagt tcatgatttc
      901 cattcggatg ctcccatcga ccagttccag gaaggcgaca taccgcttgc tgatttgagg
      961 gagttgatca tcaacaagcc cctcaaaatg ccaacggact cgatcactga ccagagactc
     1021 ctcgagatca gaaagaaata ccgccgggct tatgaaccct ggagcaatga ggaggatgaa
     1081 tggctcctga ggtcacacaa agaaaagcag agcaatgctg ctcttgcaaa gacactggaa
     1141 aggcagccga gtgccataag atcgcgtatt cgaaaattta caggataagg ccggagtaaa
     1201 cccggatgga ttttgagaca ttgcttgctt cgagatcgtc aggtcttgct cgctgagaac
     1261 aaagcgttga aacaggaaaa tctttctctg aagttacggc tggggctggt ggagctcctg
     1321 gaatgcaagc cttctccaga taagattcaa caggacgtcc tccggtagtg aatcatcctc
     1381 tcccctcaat cctcaacgcc aacgcgaatc ccgcagaaaa gatccggcta ttcatgtcct
     1441 tgttcaaggg tcgggacgac gggtatgcca aaagatggga gagcaaggac ggcgccaagt
     1501 ccggatactc acttccctgc cagaatgaat ggaagcgaga gttgtgcaag aagcctcggg
     1561 ttaaatgcac cctctgccga ggcaatgctc gatgctgtgc accattcccg agctacttct
     1621 tatctcctgg ttcgatggtg agcgaatagc gggggaaact tttagatgga ggtatgagaa
     1681 aaccagttga gtggcataga cagctgctcc ggcaggactc attggccatc cccggaatca
     1741 ggccaccgat cctcttggct caagagggct acgagttcct ctactaactg cgcggcctgt
     1801 gtccgtccct gtgattctca tgtatacggc tgctgtgttc ttcgatatag ctcggcaaca
     1861 aaaggcgagt aactttcgcg attgcacacc aagtacaaat gccgccgcgc ccgggttacc
     1921 cccacgtaga actggcgtct agcctcacgc aattgcgcct cgctgcgtct ttcaaagcta
     1981 ttcggtatta catccctatt cattgcaaat aagatgaccg catcaaactc ccgtcccttc
     2041 gcgctgtgta gcgtgctcag attgagacgt cccgagcctt cgatacggcc cccgaagtgg
     2101 ctcaaaggaa ggtgattgcc agcttctttt gtagggtcgg ttcgctcaat catgagatca
     2161 atggcatccc aatcctccac aatggtccgt gctcgttttc gccagggcag cagagcttct
     2221 cttcgaaagg ccgacagcca ctcatgagtg gacatgttgc agccaatagt ggaccggaga
     2281 aaacaaatca actgtgcctg gatctgaagc tgttcctcct ttgtggcacc tggtcctaag
     2341 acaagtgtag cggcgtcagc tgccagccgc cgaaaaggtg gatttgcctc tttccaacca
     2401 cctgcgaccc aattcgcgca ggcctcaacg aatctggaca gtcgagaact ccgcttgatg
     2461 agggcattgt tatctgcacg cacaatcgga ataccgtact ccattgcctt tgcggctact
     2521 tcatcgccct gtgttgccca tcgatacaga acgccgattt gttccatcgt tgtaccgcac
     2581 ttccggatgg agggaagcaa acttgcaaag acgaagtcgg cctgagcatt cagctctccc
     2641 tttatgccat ggaattcgat ggtgcctgcc tcagcgtcat cgggacaaca gtagtctctc
     2701 tcttcaccca gggcagccat ggacgcgtga atgatgctcg tcccacagcg gtaattgaag
     2761 cgaaggcgaa tggatcggac ttccgctcgc gatgacaggt tttcaaggag cttcggattc
     2821 gctcccagaa atgaatagat agactggtcc ggatctccaa ccgcgaatag ccgaactccg
     2881 acgttgaaac aaagcagcag cacaagttca tgcaaggcat gaccgaggtc ctgatattca
     2941 tctacaaaaa gaaccgggaa cttagcacga agagaacgcc gtacccattc atgctcgcga
     3001 atgatccgga gtgccagcag aggcatgtcg tcaaaatcga tgaggtcccg gcggcgcagc
     3061 tctgcctcat aggcttcgat gaatccagca agctcggggt tcttgcctcg ccagcgggga
     3121 tgatcccgat ccacatcgcg tctgcgtttt tcctcagcaa acttccagcg ctcatgaggg
     3181 ttctcgttgc ccccgagcat gaggcggtga gcagactcga ctgccgctcg acattcgctc
     3241 ctgtt
//
LOCUS       DAOOVC010000278         3196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_16279_length_3196_cov_5.740210, whole genome shotgun sequence.
ACCESSION   DAOOVC010000278 DAOOVC010000000
VERSION     DAOOVC010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16279_length_3196_cov_5.740210"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..471)
                     /locus_tag="PLM79_18330"
     CDS             complement(55..471)
                     /locus_tag="PLM79_18330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOV88320.1"
                     /translation="MRAFFDTSAFVKRYIEEPGSERVMEICSEAEHLVLSIICLPEMI
                     STLNRLVREGKLSGEQYQQIKDLILDDFEDVEVCDITTEVIKQTIGCLEKNPLRAMDA
                     LHLGCALVVKPDLFVSSDRRQIEAAMHEGLIVEALE"
     gene            complement(468..695)
                     /locus_tag="PLM79_18335"
     CDS             complement(468..695)
                     /locus_tag="PLM79_18335"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOV88321.1"
                     /translation="MQETNVVELRKRIKHYFDAVEGGEVVRVYRNGKPIAEIVPIPKG
                     DRSWKRDVPRLTIKGLSLSREILKDRAESEA"
     gene            complement(897..1019)
                     /locus_tag="PLM79_18340"
     CDS             complement(897..1019)
                     /locus_tag="PLM79_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88322.1"
                     /translation="MSRAIEELRPDRIVLDSLVEQLLGRNRLSREALEKIFGPS"
     gene            1593..1787
                     /gene="rpmB"
                     /locus_tag="PLM79_18345"
     CDS             1593..1787
                     /gene="rpmB"
                     /locus_tag="PLM79_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOV88323.1"
                     /translation="MSRRCEFCGKTPHVGNNVSHANNKTKRLWYPNLQQVRHMDGDGS
                     VRRVRACTRCIRSGIVVKPA"
     gene            1790..2608
                     /gene="ubiE"
                     /locus_tag="PLM79_18350"
     CDS             1790..2608
                     /gene="ubiE"
                     /locus_tag="PLM79_18350"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOV88324.1"
                     /translation="MTKPRVSMTEIPPRPRCVPSEEGPPETGLTPFGYLRVPEEEKVS
                     WVRRHFNTVASRYDFMNTLLSLGTHYIWKRLAVDLMNLGEGDRVLDVCGGTADLSLLA
                     ARQVGPTGRVVLYDINWAMMASGRPKVASSPYARNIVYVQGDAESIALESETFDAAMV
                     GFGIRNLTHMERGFREMHRVLRPGGKFMCLEFSEPTPPWFRSLYDFYSFYVMPFLGQV
                     LAGCRHAYTYLPESIRLFPSPDGLKSILEETGFSHVTYRRFTNGIAVVHLGIKG"
     gene            2618..>3196
                     /locus_tag="PLM79_18355"
     CDS             2618..>3196
                     /locus_tag="PLM79_18355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV88325.1"
                     /translation="MKLNWAERWVVNNPSRVFQQRLEIDWMSRSWPLEGGSTVLEIGC
                     GRGAGARLILKAFRPGLYHAMDLDLEMIRRARRYLSPEERRRVSLYVGDVLALPYRDD
                     SLDAVFAFGVLHHVPDWRTALHEISRVLKPGGAYFLEEIYPFVYQNFITRHILLHPKE
                     DRFFSGDLHEALEECRFEVKAARELKLAGMLGI"
BASE COUNT          632 a          974 c          896 g          694 t
ORIGIN      
        1 cgtggaattc tctgcttcat tctgctctgt tggacacttg cggcatcctc cccgtcattc
       61 cagagcctcc actatcaggc cctcgtgcat cgcggcctca atctgccgtc gatcagaaga
      121 gacgaaaagg tccggcttga ccacgagagc acaaccgagg tgcagggcat ccatggcccg
      181 caggggattc ttctcgaggc atcctatcgt ctgcttgata acctccgtcg tgatgtcaca
      241 aacctcaacg tcttcgaagt cgtccagaat caggtcctta atctgctggt actgttcccc
      301 ggaaagcttc ccttcgcgaa ccagccgatt gagggtcgag atcatctccg ggaggcaaat
      361 gatgctcagc accagatgct ccgcctcact gcagatctcc atcaccctct cgctccccgg
      421 ctcttcgatg taccttttga cgaaagcgga ggtatcgaaa aatgctctca tgcttccgat
      481 tccgctcggt ctttcaggat ctcacgactc agagagagtc ctttgatagt gagccgtggc
      541 acatcccgct tccaggaccg atccccttta gggatgggaa cgatctccgc aatgggcttc
      601 ccgttgcggt agacgcgcac cacctcgccg ccttcgactg cgtcaaaata gtgcttgatg
      661 cgttttctca gttcaacaac gttcgtttcc tgcatagctt tcacctcact ttgagaccaa
      721 atccatcact ggtattgtac gcccttaagt acatattgca actcaataaa gcgcagggaa
      781 cggtaaggct tggcggatcc aggccagcgc tcactcgaac gtcatgtgca tcaaaggggt
      841 gctttcagtc aggccatgca cgtcgtttta acaggaaacc ggcactgccg ggcccttcac
      901 gatggcccaa agatcttctc gagcgcttct ctcgataggc ggtttcttcc caggagttgt
      961 tcaacaaggc tgtcaaggac gattcgatcc ggcctcaact cctcgatggc tctactcatc
     1021 tgggtcttga ttctcctgtc cacggctttg ttcacccgta tggcaagtgg cccctccacg
     1081 gaggcttcga gaccgagggc gagcaaaccc gtttggttca tccggcagat gagtaccccg
     1141 gggttgaaag aaagtgaacg gacacggcaa gagcccaact tgttggacac caatcgaacg
     1201 ggaagggaga acaagccgtg tctacgtcga taccgtacca tgcttatggg ctgaagggta
     1261 tttgccatac ctcgactcgc tatcgtgccg attgaatcaa cttgaacagc ttgaaccggt
     1321 aaggattgga atgatcccgc ggtcttccct gcagttcccc ggcgactcgg tggtgcgctg
     1381 ccggtggcgg gcgttccacg gcctccggga gggacgggcc ccctcactct cggatccggc
     1441 cccccttcct gtcctcctcg actcatgttt tgggttgccg gaaagaaggg gctgtggtag
     1501 ataggttctt tgccaaaacg acgacacttc cggggcaatc ctcctggtac ggttgcttca
     1561 ttcatttcac ggcggaaagg aacagcaggg acatgagtcg aagatgtgag ttctgcggga
     1621 agaccccgca cgtgggcaac aacgtcagcc atgccaacaa caagaccaag cgcctgtggt
     1681 accccaacct ccaacaggtc cgtcacatgg acggggacgg atccgttcgc cgagtccggg
     1741 cgtgcacgcg ctgcatccgg agcggtatcg tggtgaagcc cgcctagaga tgactaagcc
     1801 ccgggtctcc atgacggaaa tccccccccg gccccgttgc gtcccttccg aggaaggccc
     1861 gccggagacg ggactgactc ctttcgggta cctccgcgtc cccgaagagg agaaggtgag
     1921 ctgggtccgc cgccatttca atacggtggc ttcccgctac gatttcatga acaccctcct
     1981 gagcctgggt acccactata tttggaaacg cctggccgtc gacctcatga atctcgggga
     2041 aggggaccgg gtcctggatg tttgcggagg caccgccgac ctgtcccttc ttgcggcccg
     2101 gcaagtcggc ccgacggggc gcgtggtgct ctacgacatc aactgggcca tgatggcaag
     2161 cggccgcccc aaggtggctt cctcccccta cgcccgcaac atcgtttacg tccagggaga
     2221 cgccgagagt attgccctcg agagcgaaac gttcgatgcc gccatggtcg gcttcggcat
     2281 tcggaacctc actcacatgg aacgcgggtt tcgggagatg caccgggtcc ttcggccggg
     2341 aggcaaattc atgtgcctgg agttctccga gccgaccccg ccgtggttcc gctccctcta
     2401 cgatttctat tccttttacg tcatgccctt cctggggcag gtcctcgccg ggtgtcgtca
     2461 cgcctacacc tatcttcccg agtccattcg cctttttccc agccccgacg ggctgaaatc
     2521 catcttggaa gagaccggtt tttcccatgt cacctatcgc cgattcacca atggaatcgc
     2581 cgtggtccat ttgggaatca aaggatgaag tgccgggatg aagctcaact gggccgaacg
     2641 atgggtggtg aacaatccat cccgagtgtt tcaacagcgg cttgagatcg attggatgag
     2701 ccgatcgtgg cccctggagg gcggctccac ggtcctcgag atcggctgtg gaaggggagc
     2761 cggggctagg ctgatcctga aggcattccg gccgggtctc tatcatgcca tggacctgga
     2821 cctggagatg atccgccggg cccggcgcta cctgtcccct gaagaaaggc gcagggtttc
     2881 gttgtacgtg ggagacgtgc tggccctgcc gtaccgggac gacagcctgg atgcggtgtt
     2941 tgccttcggg gtcctccatc acgtgcccga ctggaggacg gccctccacg agatttcccg
     3001 ggtactcaaa cccggggggg cttattttct cgaagaaatc tatcccttcg tctaccagaa
     3061 tttcatcacc cggcatatcc tcctgcatcc caaagaagac cgcttcttca gcggagacct
     3121 gcacgaagcc ctggaggagt gcaggttcga ggtcaaggcg gcccgggaac tgaagttggc
     3181 gggaatgctg ggaatc
//
LOCUS       DAOOVC010000279         3188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_16337_length_3188_cov_4.143632, whole genome shotgun sequence.
ACCESSION   DAOOVC010000279 DAOOVC010000000
VERSION     DAOOVC010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16337_length_3188_cov_4.143632"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLM79_18360"
     CDS             <1..215
                     /locus_tag="PLM79_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88326.1"
                     /translation="SRLFKIVDRMVPTPLSPRWSGWLWENMEEHDIRFFLPDPGPFDV
                     CRYVELPADLEERLLDDLKSGALSLH"
     gene            595..2742
                     /locus_tag="PLM79_18365"
     CDS             595..2742
                     /locus_tag="PLM79_18365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOV88327.1"
                     /translation="MNTLKRAPFPKQAETVLALLKGFKKDGTKGLFLTAEMGTGKTLM
                     AIAVSFILCPPRSRTLVMCPGHLVRKWIREIEETIPHAKTVNLNGPGLAELFELRRTA
                     PAGREFYIIGKERSKNHYARASGVMVRRHFQPICPRCGSMVDPVNPKVRKDICQSCSE
                     PLWQADGKKGFRRFAKAEFVKRYFRRGAFDLFIGDEVQQYKSGDSAQGQAFANFAAIS
                     RHVLCLTGTLMGGYVTNLFYLLWRTNPHRMKDVTGSYKSVQRFAERYGVLEEIIKEPL
                     SDNSNSIGGKRTVTVRERPGISPLVFTDLLLEQSVFMRLSDISVHLPPYMEHVVSVEM
                     TTEQSEAYTEFEDTLRKEVRQALARGDRSLLGAMLQSLLAYPDGARRGETVTHPRHLT
                     PDGSRVLVASAPRLDIDLLPKEEKLLEILKQEKSRGRRVLLYLEHTGTRDLIPDLAER
                     IGQSGLSALVLRQHTTSAENRETWVKARLSEGDYELLVCNPRLVETGLDLVEFPTIIF
                     FQCGYSTFTLRQASRRSWRIGQRHPVEVYYLSYAGTMQETALSLMGTKMQVALMVEGE
                     LSDRGLTALAEGDSSMLVALAKSLVGDEEVAPLSEAWTELMRAGINAESLIGAEDNEK
                     ETVTVEKGDRKATLAFERIVRGRVYVRKGYAVGYVDGHRFIFRNGKVTYKDNRIVGEY
                     HRNGTGTINEKPFQLVSSGNGREYMLVELRATA"
     gene            2856..3041
                     /locus_tag="PLM79_18370"
     CDS             2856..3041
                     /locus_tag="PLM79_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88328.1"
                     /translation="MDQDEENLLYLELTHIRALIWDDADDVAQEAMAESPAEVAAFAR
                     QWILKQQGKPYEASYFR"
BASE COUNT          771 a          960 c          837 g          620 t
ORIGIN      
        1 aaagccggct cttcaagatt gtagaccgta tggttccaac cccgctcagt cccagatggt
       61 ccggatggct gtgggagaac atggaggagc atgatatccg gttcttcctt cccgatcccg
      121 gcccattcga cgtatgccgg tatgtggagc tccccgcaga tctggaggag aggcttctcg
      181 atgatctcaa atccggcgcc ctctccctgc actgatcccg gttaaagctc tgcgttgcgc
      241 gctcaaaggc gttccgttca ccgcgaaggc gccaagagcg caaagaccca caaagaaatg
      301 acttcctcaa aaaggataga aggaaccaac tcccatgaaa cggttggcag cattgcatga
      361 cccttagcga ggctttgagc tctccgcatc tttgcggtga agggttgcgc gccggccact
      421 tccagcctcc ggattcccat cggcaaccca atcccgcaaa cgagagccgt catgacactc
      481 aaggatttcc tcgcacagtt tggaaacact ctcaaagaaa tcatctcgaa gcgaaacgtc
      541 ccaacctatg atcccatgaa tgaaggtcca tgggaagaag ccatcaggca tcgcttgaat
      601 accctcaagc gggctccctt ccccaagcag gccgaaacgg tcctggccct cctcaaaggc
      661 ttcaagaaag acggaacgaa agggctcttc cttacggccg aaatgggtac tggaaaaaca
      721 cttatggcta ttgccgtttc cttcattctc tgtcctccga ggagccgaac cctcgtcatg
      781 tgcccaggcc atcttgtacg aaagtggatc cgcgaaatag aggagacgat cccccatgca
      841 aagacagtga acctcaacgg cccggggctt gccgagctgt ttgagcttcg gcgcaccgcg
      901 ccggccggaa gggaattcta catcatagga aaggaacgct cgaagaacca ctatgccagg
      961 gcttccggcg tgatggtcag aagacatttc caacccatct gccctcgctg cggctcgatg
     1021 gtcgatccgg tcaacccaaa agtgaggaag gatatctgcc aatcgtgctc tgaaccgctc
     1081 tggcaggctg acgggaaaaa gggtttcaga agatttgcca aggccgagtt cgtgaagcgc
     1141 tacttccgca ggggagcttt cgaccttttc attggtgatg aggtgcaaca atacaaatcc
     1201 ggcgattccg cccagggaca ggccttcgcc aatttcgccg ccatctcgcg acatgtcctg
     1261 tgcctcaccg ggaccctgat gggcgggtat gtgacaaatc ttttctatct tctgtggcgc
     1321 acaaacccgc atcggatgaa ggatgtcacc ggcagctaca aaagcgtcca gcggtttgcg
     1381 gagcgctacg gcgtcctcga ggagatcatc aaggaacctc taagcgacaa ctccaattcc
     1441 atcggaggca agcgcaccgt cacggtgaga gaacgcccgg gaatctcgcc cctcgtattc
     1501 acagaccttc tcctggaaca gtccgtcttc atgcgcctct cggacatatc ggtccacctt
     1561 ccgccctaca tggagcacgt ggtctcggtc gaaatgacaa ccgagcagag tgaagcctac
     1621 accgagtttg aagataccct gagaaaagag gtccgccagg cccttgcccg cggggaccgc
     1681 tcgctccttg gggccatgct ccaatcactc ctcgcctatc ccgatggagc aaggcgggga
     1741 gagaccgtaa cacaccctcg ccatctaacg cctgatggat ctcgagtact ggtagcttcc
     1801 gctccgaggc ttgatataga tctcctccca aaagaagaga agctcctgga gatcttgaag
     1861 caggagaaga gtcgcggaag aagagtcctg ctctatctcg aacacaccgg gacaagggat
     1921 ctcattccgg acctggcgga acgtatcgga caatccgggc tctcagccct tgtgctgcgc
     1981 cagcacacta cttcggccga aaaccgcgag acctgggtga aagcaaggct ctcagaaggt
     2041 gactatgaac tgctcgtctg caatccccgc ctggttgaga ccggtctcga cttggtcgaa
     2101 ttccccacca tcatcttctt ccagtgcggc tactcgacct tcactctccg ccaggcgagc
     2161 agaaggtcat ggcgcatcgg gcaaagacac cctgtcgaag tctactacct ctcctatgcc
     2221 gggacgatgc aggaaaccgc cctcagcctc atgggaacca agatgcaggt agccctcatg
     2281 gtcgaaggcg agctctcgga tagaggcctc acagctcttg ccgaaggcga ttcctccatg
     2341 ctcgtagcgc ttgccaaaag ccttgtcggc gatgaagaag tagcgccctt atccgaagca
     2401 tggactgagc tcatgagggc aggcatcaat gccgaaagcc tcataggcgc tgaagataac
     2461 gagaaagaga cggttaccgt cgagaagggt gaccgcaagg caacgctcgc cttcgagcgc
     2521 atcgtacgag gcagggtcta cgtccgaaag ggctatgccg ttggctacgt tgacggccac
     2581 aggttcatct tcagaaacgg caaggtcacc tacaaggaca accgcatcgt cggtgagtac
     2641 caccgcaacg gcaccggcac catcaacgag aaacccttcc agctcgtctc atccggaaac
     2701 ggccgcgaat acatgcttgt tgagctgagg gcaactgcgt agccgcccgg ctccgccagc
     2761 caccaccgca agcaaagagc ttcttcaaca ctcgatagag catctctctt tacaacgatg
     2821 cctccggaac caccaaatca cacgggatga cgatcatgga tcaggatgaa gaaaacctct
     2881 tgtacctcga gctgactcac atcagggcac tcatctggga tgatgcagac gatgtcgcac
     2941 aggaagccat ggcggagtca ccggccgaag tcgccgcctt cgctcgtcag tggattctga
     3001 aacagcaggg caagccctat gaagcaagct atttcagata aggcgccttt tggcttcatc
     3061 ccattggcag attttcccgc tccgccaggc actctcggct tggtcaactc ggcaaaccgg
     3121 tacgatttca gcctgcaaca gaggtaacag catgaacgat tacagcctct ataatgcgat
     3181 ccttggcc
//
LOCUS       DAOOVC010000280         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_16681_length_3138_cov_3.836847, whole genome shotgun sequence.
ACCESSION   DAOOVC010000280 DAOOVC010000000
VERSION     DAOOVC010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16681_length_3138_cov_3.836847"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLM79_18375"
     CDS             <1..274
                     /locus_tag="PLM79_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88329.1"
                     /translation="KARVAASLFMKALAVNVKRFVHYGSAKDEKHGPRTLRGTAAFIF
                     QQNPMPFVQLERLAFRPYPRFCRMTHSPAIFGDAAGVLGSYDTIID"
     gene            669..1562
                     /locus_tag="PLM79_18380"
     CDS             669..1562
                     /locus_tag="PLM79_18380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88330.1"
                     /translation="MKVVFNDVVGRRLVARIVDPFSGTEEALLDAPIQVLSPDGKTAL
                     SLNYKRLYRLRPEYGYAPEVNNFSADMEPTGDGIWKVDLLSGTTCLILSLEQLTTFQP
                     RQDMRGAAHKVNHLLYSPGGERFAFMHRWIGPKGKFSRLYTANADGSDLRLLADDRMV
                     SHYAWKDSGQLLAWARKEPWGDHYYLFEDPSGTFRIIGEGVFDLHGDGHPGFSPDGRW
                     IVTDTYPDKARMRALLLWDTHRERVVTVGRFFAPWRFDEAQRCDLHPRWHPNGKMLSI
                     DSVHEGIRRTYVINVEQIVAS"
     gene            1618..2661
                     /locus_tag="PLM79_18385"
     CDS             1618..2661
                     /locus_tag="PLM79_18385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOV88331.1"
                     /translation="MSLVVVVRNEKHVVTSAVDSLVGQNYPRELLEIILVDGLSEDGT
                     AEILAQTFESLERRGWRVSLFENPRHILATGWNIAVRASLGDLMCRIDAHSELYPDYV
                     RRGVEVLQHTEDTRIGAVGGVLEHYGRGLIGEAVADLLASRFSVGNSPFRTDKDKRAR
                     LEKCRETDTAVYALYPKRIFEEIGYFDEGLARNQDIEFHERVKAKGYRFLTCPDMRIK
                     YHVRNTVRALVKKAHSDGYWTAFLRKASLRHRIPAFFALYLIFYGLLCAASWGLRGAT
                     TASLVLCGLPLILYLLMTGFYALKDGRGVTRLLLCGLFPLYHLSYGLGTLKGMAVRGI
                     RPGTPPMSRNAPW"
     gene            2655..>3138
                     /locus_tag="PLM79_18390"
     CDS             2655..>3138
                     /locus_tag="PLM79_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOV88332.1"
                     /translation="MVRRHTRLSEGRVPAARLDGDVLQAYALKLSVRLRAKSRVFLFW
                     KGRVALYALLRAMGVGAGDEVVLSAYTCVVVPNAVFYCGAVPQYVDVAPQTYCMDVRD
                     VEKALGPRTKVVLCQNTYGLSANVEEISGLARSRGIYTIEDCAHGFGGSFRGKPNGSF
                     C"
BASE COUNT          630 a          844 c          930 g          734 t
ORIGIN      
        1 caaagcccgt gtggccgcct ccctgttcat gaaggcactg gccgtcaatg tgaagcgatt
       61 tgtacactat ggctctgcaa aagatgaaaa gcacggacca aggaccctcc gagggaccgc
      121 tgcctttatt tttcagcaaa atcccatgcc ttttgtccag ctggagagac tggctttcag
      181 accataccct cgcttttgcc gcatgacgca ttccccggcc atcttcgggg atgcggcagg
      241 cgttttgggt tcttacgata ccatcataga ttgaccttat gcagtacggt tccttagaaa
      301 ggcgaatcgc cacagccctg gacggcttcc cgaatttgcg gaggggggtc aagttcctat
      361 atcagcgatg caattacctt ctgagcggga aatccgctgt atcggtcgat ttgaggccgg
      421 agtgcagtat ccgtcccgtt ctgcaaggtg tgcaaggaga tcccggctgt tttttcggtt
      481 atttcgacaa atctccctgg tcaccttgca gcaggtatta cctgcttcac gtgctttccg
      541 aagagtccgg cggtcgtgtg cgccttgctt gttacgactt cgaaacgaac gacacgaccg
      601 tctttcaaga aagtcgtgca tggaattatc aacagggatg tatgctgcag tggcagccag
      661 gggatggttt gaaggtcgtg tttaacgacg tggtgggaag aagactggtc gcgcgcatcg
      721 tggacccttt cagcggaacg gaagaggccc tgctcgatgc gccgatccag gtgttgagcc
      781 ctgacgggaa aacggcgctt tctctgaact acaagagact ttacaggctt cggccggaat
      841 acggctatgc cccggaggtt aataactttt cggcggatat ggaaccgacc ggggacggca
      901 tttggaaggt ggatcttttg tccgggacaa cctgcctgat tctctccctg gagcaactga
      961 caacgtttca gccaagacaa gatatgcgag gtgcggccca caaggtgaac catctacttt
     1021 attcgccggg cggggagcgt tttgcattca tgcaccgctg gatcgggccc aaggggaagt
     1081 tttcaaggct ctacacggcc aatgcggatg gttcggattt acggcttctt gcagatgatc
     1141 ggatggtttc ccattatgca tggaaagatt caggacaact tttggcctgg gcccgcaagg
     1201 aaccatgggg tgatcattac tatctttttg aagacccttc ggggacgttc aggatcatcg
     1261 gcgaaggcgt tttcgacctt cacggggacg gccatcccgg tttttctccg gacgggcgtt
     1321 ggatcgtgac cgacacctac ccggacaaag ctcgcatgcg agctctgttg ctctgggaca
     1381 cccaccggga gcgtgttgtg acggtggggc ggttcttcgc cccgtggcgt tttgacgagg
     1441 cgcagcgctg cgacctccat ccccgttggc acccgaatgg gaagatgctt tccatcgact
     1501 ccgttcatga ggggattcgc cgaacctatg tcatcaacgt cgaacaaatc gtcgcttctt
     1561 gagactcgtc gcaagggggc cccgagttca gcgtacgaca agaggggact tcctgacgtg
     1621 agcctcgtcg tggtcgtccg caacgaaaaa catgtcgtga cgagtgccgt cgattctctg
     1681 gtagggcaga attaccccag ggaacttctg gagatcatcc tggttgacgg gctgtccgaa
     1741 gacgggacgg ccgagatttt ggcccagacc tttgaatcac tggagcgccg ggggtggcgg
     1801 gtgagccttt tcgaaaatcc ccgacacatc cttgcaaccg ggtggaacat cgccgttcga
     1861 gcctcactcg gtgatctgat gtgtcgtatt gacgcccaca gtgagcttta ccccgattat
     1921 gtccgaagag gcgtagaggt tctgcagcac accgaagaca caaggatcgg agctgtaggg
     1981 ggggtattgg agcattacgg tcgggggctc ataggagagg ccgtcgccga tctcctggct
     2041 tcgcgctttt ccgtcggcaa ctcgcctttt cgaaccgaca aggacaagag agccaggctc
     2101 gagaaatgcc gcgaaacgga tacggcggtc tacgccctct accccaagcg gatttttgag
     2161 gagataggct attttgacga gggactggct cgcaaccagg acattgaatt ccacgagcgt
     2221 gtcaaagcca agggataccg gtttctgacc tgcccggaca tgcgcatcaa gtaccatgtg
     2281 cgcaacacgg tccgcgctct cgtgaagaaa gcccactccg atggctactg gaccgccttt
     2341 ttgcgaaagg cttcccttag gcaccgcatc cccgcgttct ttgcccttta tctcatcttc
     2401 tacggcctcc tctgcgcggc atcctggggt ctgagaggcg caaccaccgc ctccttggtg
     2461 ctttgcggct tgcctttgat cctttacctt ctcatgacgg gattctatgc actcaaggac
     2521 ggcaggggcg tcacacggct cttgctctgc ggcttgtttc ccttgtatca tctgtcctat
     2581 ggattgggta cgctcaaggg catggcggtg cgaggaatcc gcccaggcac gccgccaatg
     2641 agccggaatg caccatggtg aggcggcaca cccgactgag tgagggacgc gttcccgccg
     2701 cgaggctgga tggggacgtt ctgcaagctt atgccctcaa gctgtccgtt cgcttgaggg
     2761 caaaatcccg ggttttttta ttctggaaag ggcgggttgc tttgtacgcc ttgctgcggg
     2821 ccatgggggt cggcgctggc gatgaagtgg tgctttcagc ctacacttgt gtggtcgtgc
     2881 ccaatgcagt cttttattgc ggtgccgtgc cgcaatacgt ggacgttgca ccccagacgt
     2941 actgcatgga cgtccgtgac gtggaaaagg ccctaggtcc aagaacgaaa gttgttctgt
     3001 gccagaacac ttatgggctt tccgcaaacg tggaggaaat cagcgggctt gctcgatcgc
     3061 gcgggattta cacgatcgag gattgcgccc acggatttgg gggctctttt cggggaaaac
     3121 ccaacgggag tttttgtg
//
LOCUS       DAOOVC010000281         3117 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_16821_length_3117_cov_6.377858, whole genome shotgun sequence.
ACCESSION   DAOOVC010000281 DAOOVC010000000
VERSION     DAOOVC010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16821_length_3117_cov_6.377858"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLM79_18395"
     CDS             <1..409
                     /locus_tag="PLM79_18395"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOV88333.1"
                     /translation="PADLSEVQVLMSRGRIVLTRTRRWSGVYGVLILEANDPMAQLAE
                     VARRVPLYEDEVRFLSRCVRCNVLIHDVPRSRVFGHVPDYTYETQERFAQCPSCGRFY
                     WAGSHPSRMQEHLQSTLRWKADSLRGPGNGPIR"
     gene            454..858
                     /locus_tag="PLM79_18400"
     CDS             454..858
                     /locus_tag="PLM79_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88334.1"
                     /translation="MNQGGGMVAGMLKRVWYFGMERFWIVYQWKLRFFRLQLKKWKRC
                     KERKDLDQAYADLGSEVYGLYRQEVTDWAQAENVQYRLRQVEEAESRLLAVEDAMERI
                     RKEYGERREEIRSNYAAKREALGVTVEDDEDR"
     gene            complement(1131..1640)
                     /locus_tag="PLM79_18405"
     CDS             complement(1131..1640)
                     /locus_tag="PLM79_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HOV88335.1"
                     /translation="MSEKRFRRTVAIITVSDKGSRGEREDASGNALEQYFESRGFQVV
                     HREIVPDEVREIARSIVQSVDEKKAALVVTTGGTGVAPRDVTPEATLQVLDRSVPGMA
                     EAMRATSLAKTPHAMISRAVVGIRKEALIVNLPGSPKGALENLDAVMPAVPHALEKIQ
                     GDPSECAAS"
     gene            complement(1768..>3117)
                     /locus_tag="PLM79_18410"
     CDS             complement(1768..>3117)
                     /locus_tag="PLM79_18410"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOV88336.1"
                     /translation="EKRILRYLGDWGHPKARLDMKATLDKRSNTAQVMVEVQTGPLCH
                     VGDITLEGNENVADKVILRELTFKKGDRFNSTKISESQQRLFNLDLFQFVDITVENLE
                     SVDSTSIPVRILVKEAKKQTVRVGVGYGTEDEFRGQVQYEVRDFLGNGRRLQVNAKAS
                     SLVQFLEGKVLQPHLFDAKSSLTLTGGVRHEDQESFENRKVYVSPLLNYKWSERLSSF
                     LGYNLEANRLLSVDLSKGALGPIDDENQEYYVSSLLMGNTWEQVDNTLNPRKGWRFLQ
                     SVEWASVGLGSEVDFVKLALEARGYLPLRKYGVLAAKLKYGGIQKLENTTDIPIFKRF
                     FVGGSDSVRGYPYQRLGLLDRRGNPIGGMTMTEGSLEWRFPIRGSFEGVLFFDAGGLY
                     EESFHLGWNDTQYTSGCGIRYLTPVGPLRLDFGYQLNPPDQDFFSPYQFHFSIGQAF"
BASE COUNT          602 a          929 c          948 g          638 t
ORIGIN      
        1 gccggccgat ctctcggagg tccaagttct catgagccgg ggaaggatcg tcctcacccg
       61 cacccgccgg tggtccgggg tttacggggt cctgatcctc gaagccaacg atccaatggc
      121 tcaactggcc gaggtggcac gccgggtccc cctctacgag gatgaggttc gcttcctcag
      181 ccgttgcgtg cgctgcaacg ttctcattca tgacgtgccc cgttcccggg ttttcggtca
      241 cgttcccgac tacacctacg agacccagga acggtttgcc caatgtccgt cctgcggcag
      301 gttttactgg gcagggtccc acccttcccg aatgcaggaa cacctgcaat cgaccctgcg
      361 gtggaaggcg gattccctga ggggaccggg caacggtccg attcgttaga caacggcagg
      421 tttggctttg actggaaatc aggaggagga aaaatgaatc aaggaggagg aatggtcgcg
      481 ggaatgctca agagagtctg gtactttggg atggagaggt tctggatcgt ctaccagtgg
      541 aagttgcgct tcttcagact gcagctcaag aaatggaaga ggtgcaagga aagaaaagac
      601 ctggaccagg cctacgcaga ccttggctcg gaggtgtacg gactctatcg gcaggaagtc
      661 accgactggg cccaggctga gaacgttcaa tatcggctgc gccaggtgga ggaagcggag
      721 tcccggctgc tggccgtcga agacgccatg gaacgtatcc ggaaggaata cggggaacgc
      781 agggaggaga tacggtccaa ttatgccgcc aagcgggagg cattgggagt gaccgtcgag
      841 gacgacgagg accggtagga ggccggggag ccgggaggga tgtagggtgg gtggagcgaa
      901 gcggggccca ccaggggggc tcggggccag gggctggaag gccgggaagg gtcaacgggt
      961 cggctcgtgg gggttcgtca tgggcctcgg gggcgggtcg gggccgggac aggcatcgaa
     1021 atccgggggg agaaccgggg tgccgagagc ccccggagga tggcggcacg gcttcccggc
     1081 gccgcgggtt cccgtttgac cccaatcctg cgggttttgc ccacccgacc ctacgaggcg
     1141 gcgcactccg aaggatcgcc ctggatcttt tccagggcgt ggggcacggc gggcatgacg
     1201 gcgtcgagat tttccagggc tcccttgggg cttcccggaa ggttcacgat gagggcctct
     1261 tttcggatgc ccaccacggc tctcgagatc atggcgtggg gagttttcgc caggctggtc
     1321 gccctcatgg cctccgccat cccgggcacc gatcgatcca ggacctgtaa tgtcgcttcg
     1381 ggagtaacat cccggggtgc gacccccgta ccccccgtgg tcaccaccag ggcggccttc
     1441 ttctcatcga cgctctgaac gatggatcgg gcgatctccc ggacctcgtc gggcacgatt
     1501 tcccggtgga cgacctgaaa cccccgggac tcgaagtatt gctccaaagc atttccggat
     1561 gcgtcctctc tctctccccg ggaccccttg tcgctcacgg tgatgatcgc caccgtgcgg
     1621 cgaaagcgtt tctcgctcat gtgaatgctc gtcctttccg atgaatttcc ggcctgtgtc
     1681 cgcacgggtc ctcacccgcg ggccaatgat cgaagaaggg ttttttcccg cccttgtttt
     1741 caacacccac ccggaaccgg agggaggcta aaaggcctgg ccgatgctga agtggaactg
     1801 gtatgggctg aaaaaatcct ggtccggagg attcagctga tagccgaagt ccagccgcaa
     1861 aggacccaca ggcgtcaggt accgaatccc gcacccgctt gtatactgcg tatcgttcca
     1921 ccccagatga aagctttctt cgtacaggcc cccggcatcg aagaacagca ctccctcgaa
     1981 ggaaccgcgg atggggaagc gccattccag gctcccttcc gtcatggtca ttcccccgat
     2041 gggatttccg cgccggtcca ggaggcccaa gcgctggtag gggtagcccc gcacgctgtc
     2101 ggacccgccc acgaagaagc gtttgaagat gggaatatcc gtcgtattct ccagcttctg
     2161 aattcctcca tacttgagct ttgccgccag gaccccgtac ttgcgaagag gcaggtaccc
     2221 tcttgcctcc agggcgagct tgacgaaatc cacctcggac cccaggccta cactggccca
     2281 ctccacgctt tgaagaaatc gccagccctt cctgggattg agggtgttgt ccacctgctc
     2341 ccaggtattc cccatgagca gggacgaaac atagtattcc tggttttcat cgtctatggg
     2401 tcccagggcc cctttgctca aatccacgga caaaaggcgg tttgcttcca ggttgtaccc
     2461 cagaaaggaa ctgagacgct cggaccactt gtaattgagc aaagggctca catatacttt
     2521 gcggttctcg aaagattcct ggtcttcgtg tctcacccct cccgtgagcg tgaggctgct
     2581 cttggcatca aacagatggg gctggagcac tttgccttcc aggaactgga ccagggaact
     2641 ggccttggcg ttcacctgca atcggcgccc gttgcccagg aagtcacgaa cttcgtattg
     2701 aacctggccc ctgaactcat cttccgtccc gtagccgacc cccacccgca cggtctgttt
     2761 cttggcctcc ttcaccagga ttcgtacggg aatgctggtg gaatcaacgg attccaggtt
     2821 ttccacggta atatccacaa attgaaagag gtccaggttg aacaggcgct gctggctttc
     2881 ggagatcttg gtggaattga accgatctcc cttcttgaag gtgagctccc tcaagatcac
     2941 cttgtcggcg acattttcgt tgccctccaa cgtgatgtcg cccacatgac agagcggccc
     3001 cgtttggacc tcgaccatca cttgggccgt gttgctccgc ttatccaggg tggccttcat
     3061 gtcgagcctt gccttgggat gtccccaatc tcccagatac cgcaggattc ttttctc
//
LOCUS       DAOOVC010000282         3085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_17064_length_3085_cov_6.777228, whole genome shotgun sequence.
ACCESSION   DAOOVC010000282 DAOOVC010000000
VERSION     DAOOVC010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17064_length_3085_cov_6.777228"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLM79_18415"
     CDS             complement(<1..580)
                     /locus_tag="PLM79_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88337.1"
                     /translation="MARKRDDRGGELRCSFCGKSQDEVKKLIAGPTVYICDECVELCN
                     DIIAEEYEREEAARATQIPKPVEIKGILDQYVIGQERAKKILSVAVHNHYKRIELKLD
                     KNDVELQKSNILMIGPTGSGKTLLAQTLARILNVPFTIADATTLTEAGYVGEDVENIL
                     VSLIQAADYDIEKASRGIVYIDEIDKIAKKSDS"
     gene            complement(1007..1606)
                     /gene="clpP"
                     /locus_tag="PLM79_18420"
     CDS             complement(1007..1606)
                     /gene="clpP"
                     /locus_tag="PLM79_18420"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010919829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOV88338.1"
                     /translation="MSLIPIVIEQSSRGERAFDIFSRLLRDRIIFLGTAINDDVANLI
                     IAQMLFLESEDPDKDIHFYINSPGGLVTSGMAIYDTMRYIKPDVSTLCMGQAASMAAV
                     LLAAGVKGKRFALPHVRIMLHQPMGGFQGQATDVEIQAREILRLREEINQILVDHTGR
                     PLDQIERDTDRDFYMSGEQAKEYGMVDEVILSRKTGIKP"
     gene            complement(1611..2924)
                     /gene="tig"
                     /locus_tag="PLM79_18425"
     CDS             complement(1611..2924)
                     /gene="tig"
                     /locus_tag="PLM79_18425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOV88339.1"
                     /translation="MNVNVVDLGAHQKRLQVEIPAEEVRQELEKKYREIGKSVRIRGF
                     RPGKIPRSILKSYYGKSIEKEVSSSFIQDTFPKALRDTDLKPLIEADVDEMKFAEDGS
                     FTYSAVVDVRPSFRVEGYKGLEIARPPVRVTEEQLEAELESLRQRHAQIRAVEADRPV
                     REGDVILVDFTPWVDGKAFKKGETKDYMMEVGKNALHPDFDRHLLGHLPGESFSFELD
                     YPEDAPTKEIAGKRVQFDVTVRELKEKVLPELNDEFAQEAGSFDSLEALKQELRGRLE
                     KREEEKSRSEVRKQILDRLVQIVEFDLSDRVVEREVDHLIGLLQRQFEAQRLKIDTTR
                     FNTPEIREEYRPQAVRNIRWRLICEQIARQEDIELTEQEIEELYAEVARMIRVDVETV
                     KSQYADSVVVEQARENRIQDKVFECIESQAVFLDPPQEAPGPQQE"
     gene            complement(2991..3079)
                     /locus_tag="PLM79_18430"
     tRNA            complement(2991..3079)
                     /locus_tag="PLM79_18430"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3043..3045),aa:Leu,seq:tag)
BASE COUNT          571 a          886 c          895 g          733 t
ORIGIN      
        1 ggctgtcgga tttcttggcg atcttgtcga tttcgtcgat gtagacgatt ccccgggagg
       61 ctttctcgat gtcataatcg gcggcctgga tgaggctcac caggatgttc tcgacgtctt
      121 ctcccacgta gcctgcttcg gtaagggtcg tggcgtccgc gatggtgaag ggaacattga
      181 ggatcctggc aagggtttgg gccagaaggg tctttccgga gcccgtggga ccgatcatga
      241 ggatattgct cttctgcagc tccacatcgt tcttgtccag cttgagctcg atgcgcttgt
      301 aatggttgtg cacggcgacg gaaaggatct tcttcgctcg ttcttgaccg atgacatact
      361 ggtccaggat gcccttgatc tcgacgggct tggggatctg ggtggccctg gccgcctcct
      421 cccgttcgta ttcctcggcg atgatgtcgt tgcagagctc cacgcattcg tcacagatgt
      481 agaccgtcgg cccggcgatc aacttcttca cttcgtcttg gcttttccca cagaaagagc
      541 acctaagctc tccgcctcga tcatcccgct tcctagccat gaaaaatcct ccttggccat
      601 cttgtacgac accttcaccg acaggaaacc gcttcggtgt aaatgattgg gttagcaaca
      661 ggcgtgggct tggagggcgc cacccctcga gtttgacgct cattccggga atgcgggccc
      721 ctgtttcctc gtgcagaatg tagaccggcg cccgcagccg tcccacatca tcacaggtaa
      781 cctttggaaa agaaaaggtc aatttctttc tttgtccaga ggcctatgca taggaaatcg
      841 gatttcacgg cggatgggat gaggggtttt ccaggggcgg gggcaaaaag aagggaacga
      901 ccgaaaagga tcgggcctga gcgccatggc agccgccggg tcccatgatg gaatcccccc
      961 cggcaggaag agcttgccgc ccgccgaggg gaaaaaacgg ccaaggtcag ggtttgatgc
     1021 cggtctttcg actgaggatt acctcgtcca ccattccgta ctctttggcc tgttcgccgg
     1081 acatgtagaa gtcgcgatcc gtgtcgcgtt cgatctgatc gaggggtcgt cccgtatgat
     1141 ccacaaggat ctggttgatc tcttccctca ggcgcaggat ctctcgggcc tggatctcga
     1201 cgtccgtggc ctgcccctgg aaaccgccca tgggttggtg gagcatgatt cgtacatggg
     1261 gaagggcgaa ccgttttccc ttgacccccg ctgcgagcaa tacggcggcc atgcttgccg
     1321 cctgccccat gcacagggtg gaaacgtcgg gcttgatgta ccgcatggtg tcgtagatgg
     1381 ccatgcccga cgtgacgagg ccgccggggg aattgatgta gaagtgaatg tccttgtccg
     1441 ggtcctcgga ctcgagaaaa agcatctggg cgatgatgag gttggccaca tcatcgttga
     1501 tggccgtacc cagaaagatg attcggtccc gcaacagtcg ggagaagatg tcgaaggccc
     1561 gctccccgcg actgctctgt tcaatgacga tgggtatgag agacatgaat ttactcctgc
     1621 tggggtcctg gggcttcctg cggtgggtcc aaaaacaccg cctgggattc gatgcattca
     1681 aagaccttgt cctgaatccg gttctccctg gcttgttcca cgacgacgct gtcggcgtat
     1741 tggcttttca ccgtttccac gtccacccgg atcatacggg cgacttccgc gtagagctcc
     1801 tcgatctcct gttccgtgag ttcgatgtcc tcctgtcggg cgatttgctc acaaatcaag
     1861 cgccagcgta tattccggac ggcctggggc cggtactcct ctcgaatctc cggcgtgttg
     1921 aatctggtgg tgtcgatctt gagtctctgg gcttcgaatt ggcgctggag gaggccgatg
     1981 agatgatcca cctccctctc gacgactcga tcggacaggt cgaactccac aatctgcacc
     2041 aggcgatcca agatctgctt gcggacctcg gagcggctct tctcttcttc ccgcttttcc
     2101 agccggcccc gcagttcctg cttcagggcc tcgagggaat cgaaggagcc cgcttcctgg
     2161 gcgaattcgt cgttcaattc cggcaggact ttctccttga gctcccggac agtcacgtcg
     2221 aactgaacgc gcttgcccgc gatctcctta gtgggagcgt cttccgggta atccagttcg
     2281 aaggaaaagg attcccccgg caaatgtccc agcagatgac gatcgaagtc cggatggagg
     2341 gcgttcttgc cgacctccat catgtagtcc ttggtttccc cctttttaaa ggccttgccg
     2401 tccacccagg gggtgaaatc caccagaatg acgtctcctt ctcgaacggg ccggtccgcc
     2461 tcgacggcac ggatctgggc atgccgttgt cggaggcttt cgagctcggc ttcgagttgt
     2521 tcttcggtta cccggacggg ggggcgggcg atctcgaggc ctttatagcc ctccacccgg
     2581 aacgaaggtc gcacgtccac cacggccgaa taggtgaagc tgccgtcttc cgcaaatttc
     2641 atctcgtcca cgtccgcctc gatgagaggc ttgagatccg tatcccggag ggctttgggg
     2701 aaagtgtcct ggatgaagct cgaggagact tctttttcaa tggacttgcc gtagtaggac
     2761 ttgaggatgc tgcgggggat cttgcccggg cgaaagcccc gtatgcgtac gctcttgccg
     2821 atctcgcggt acttcttttc caattcctgc cggacttctt cggcggggat ttcaacttga
     2881 agtctcttct gatgcgcacc cagatccacg acgttgacgt tcatgcccac ttcttaacct
     2941 ttcctttgtt ggataaaata aagctctatg caaccgacac gagagaatgc tggtgcgaga
     3001 ggggggagtt gaacccccat cctgcgtcaa cagggctgga tcctaagtcc agtgcgtctg
     3061 ccggttccgc cactctcgcc gggaa
//
LOCUS       DAOOVC010000283         3067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_17206_length_3067_cov_5.251328, whole genome shotgun sequence.
ACCESSION   DAOOVC010000283 DAOOVC010000000
VERSION     DAOOVC010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17206_length_3067_cov_5.251328"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLM79_18435"
     CDS             complement(<1..649)
                     /locus_tag="PLM79_18435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOV88340.1"
                     /translation="MDPVWYENYDAGVPRSLDNSDPLLPHQLELMAGRFPRNVATEFF
                     GAKLTYGELWDQVLRMAGAFLEMGAGRGKKVAIMLPNCPQTIIAYYAALWNGAVPVLT
                     NPLYVEREMVHQWTDSETEYLVVLDHLYPKVRNVLGKTGIKKVIVTSLREYLPRLYRL
                     LYPIKARRGNLFTAVPYGEDILNFTRLIRSHEPSTLPCTVEPDDLAVLQYTGGTTG"
     gene            complement(1161..2150)
                     /locus_tag="PLM79_18440"
     CDS             complement(1161..2150)
                     /locus_tag="PLM79_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOV88341.1"
                     /translation="MSGCVLVVMEIRGGKFRRVSYEVAGEGKRLADRLGSDVCAVALG
                     SGISGEAVGLGRYGVDKVYVLDDPSLEHYVPEVYSALVAEVVQRCNPCVVLLPASVNG
                     GDLSARLAGRLEAGLAQDCTQILCEDGKIKAKRPVFSGKCFAWCEWAPGALPLISCRP
                     NVMDCLAPQEQKRAEMETLQLPIPERRSEVRTMDLDTTGKVELTEAEIIVSGGRGMKG
                     PENYALLQELAQILGAAVGASRAAVDAGWRPHSDQVGQTGKVVNPNLYIAVGISGAIQ
                     HLAGMGSSKYIVAVNKDPEAPIFSKADYGVVEDLFTFLPVFNEEMKKFKSTCS"
     gene            complement(2172..2969)
                     /locus_tag="PLM79_18445"
     CDS             complement(2172..2969)
                     /locus_tag="PLM79_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOV88342.1"
                     /translation="MNIAVLLKQTPDTESVIRIAGNGRSIVTDDLKWIINPYDEFAVE
                     AALRLKEKLGGVVSILSVGPQRTVEAIRTALAMGADNGVLIDDPAMEGSDSLAMARVL
                     ATAARELNPDLILCGSRAVDFDQAQIGAQVAEFLGRPHWSLAVSIECDGSRVIIERPV
                     EGGKSVAVADLPALITLGGSHAVWNPRYASLPGIMKAKKKPLTFKKLSDLGIDPSQCG
                     PHAAKIRMTALEMPPVRKPGRILDGNLDAAGKARELVRLLHEEAKVI"
BASE COUNT          589 a          979 c          882 g          617 t
ORIGIN      
        1 tccccgtggt ccccccggta tactgaagga ccgccagatc gtcgggttcc accgtgcaag
       61 gcaaggtcga gggctcgtga ctccgaatga gccgggtgaa gttcaggatg tcttcgccgt
      121 aaggcacggc ggtaaaaagg ttccctcttc tggccttgat gggatacagc aggcggtaca
      181 gtcggggcag atattcccga aggctggtta cgatcacctt cttgatgccc gttttgccga
      241 ggacgttccg gaccttgggg tacaggtgat ccaacaccac caggtactcg gtctccgagt
      301 ccgtccactg gtgcaccatt tcccgttcca cgtacaaggg atttgtgagt acgggaacgg
      361 cgccgttcca cagggccgcg taataggcga tgatggtctg ggggcagttg ggcagcatga
      421 tggcgacctt cttcccccgc ccggctccca tttcgaggaa ggcgccggcc atgcgcaaga
      481 cctggtccca gagctctccg tacgtcagct tggccccgaa gaactccgtg gcaacgttgc
      541 gggggaatct cccggccatc aactccaact gatggggcag caacgggtcc gaattgtcca
      601 gggagcgagg aacaccggca tcatagtttt cgtaccagac aggatccatt cggctctccg
      661 attccgtgag acaaccatct catccttttt ccggagtgag catccagcag gctgaaaaac
      721 ccggcactcc gttttccaag aagcgatcct actctccttc gagcgatttg accgggacga
      781 atcccgtttc gtcgttcccg cgcaggcggg aatccggaaa gagcgtgcat acccgctgga
      841 tgcccgcctt ggcgggcatg ccgcccggat gttctcggaa tcgctccact caggcagcag
      901 ccaatcgctc aatcggggcc tgctatcctc atcccgtaac gggatggtta gggaggaagg
      961 ggacgacaac cgacgaacca cctttccccc ctcgccactc atcgaggggg aaagaaggtg
     1021 cacccgagtc tcgaggtcct gccgagaacg gtttacgaac taaagaaccc cacggccctc
     1081 tcccgtcgcg gggacggaga gcttcccttc cttgacggca gttggacgga cggggggaac
     1141 gacctgcatg gttccaagcc ctaggaacag gtggacttga atttcttcat ctcttcgttg
     1201 aatacgggca ggaaggtgaa aaggtcttcc accactccat agtccgcctt gctgaagata
     1261 ggcgcttcgg ggtccttgtt cacggctacg atatacttgg acgaacccat cccggccaga
     1321 tgctggatgg ctccggaaat ccccacggcg atatagaggt tcggattcac caccttgccc
     1381 gtctgaccca cttggtcgga atgaggtctc cagcccgcat ccaccgccgc ccgggatgct
     1441 cccacagcgg cacccaggat ctgcgccagt tcctggagca gggcgtagtt ctccggtcct
     1501 ttcatgcctc ttcccccgga gacgatgatc tcggcttcgg tgagttccac tttccccgtg
     1561 gtgtccaaat ccatggtccg gacctcggag cggcgttcgg gaatcgggag ctgcagggtt
     1621 tccatctccg ccctcttttg ctcctggggg gcaagacaat ccatgacatt gggacggcag
     1681 gagatgagag gcagcgcccc gggggcccat tcgcaccagg caaagcattt tcctgaaaag
     1741 acggggcgtt tggctttgat ctttccgtcc tcgcatagga tttgggtgca atcctgggcc
     1801 agccccgctt ccagccgtcc ggcaagacgg gcgctcaagt ccccgccgtt caccgatgcg
     1861 ggcaagagca cgacgcacgg gttacaccgc tgcaccacct cggccacaag ggccgagtaa
     1921 acctcgggaa cgtagtgctc cagggaagga tcatccagga cgtagacctt gtccactcca
     1981 taccggccca gtcccaccgc ctccccggaa atcccggaac ccagggcaac ggcacacaca
     2041 tccgatccca gacgatcggc caacctcttg ccctcgcctg ccacctcata cgatactcgg
     2101 cgaaacttcc cgccgcggat ttccataacg accaaaacgc aaccggacat ggtctgctcc
     2161 tgtgtcaacg gtcagatcac cttcgcttcc tcgtgcagca ggcgaaccag ctctcttgct
     2221 tttcccgcgg cgtcgaggtt tccgtcgagg atgcgaccgg gctttctcac cgggggcatc
     2281 tccagggccg tcatgcggat cttggcggcg tggggaccgc attgggaggg atcgatgccc
     2341 aggtcggaca gctttttgaa agtcaggggc tttttcttgg ccttcatgat gccgggcagg
     2401 gacgcataac gggggttcca tacggcatgg gaacctccca gggtgatgag agcgggcaga
     2461 tccgccacgg cgacgctttt tcccccttcc accggacgtt cgatgatcac ccgggagccg
     2521 tcacattcga tgctgaccgc cagggaccag tggggccggc cgagaaactc cgctacctgc
     2581 gctcctatct gcgcctggtc gaaatccacg gcccgggacc cgcagaggat gaggtccggg
     2641 ttcagttccc gggcggccgt ggccagaacc cgcgccatcg ccaggctgtc gcttccctcc
     2701 atcgccgggt cgtctatgag aaccccgttg tccgctccca tggccaaagc ggttcgaatc
     2761 gcctccacgg tcctttgagg tccgacgctc aggatgctca ccactccccc gagtttctcc
     2821 ttcaaacgga gggccgcttc taccgcaaat tcatcgtaag ggtttatgat ccatttgagg
     2881 tcatccgtga cgatggacct gccgttgccc gcgatgcgaa tgaccgactc cgtatccggc
     2941 gtctgtttga gtaggactgc aatgttcacg gctatttctc ctggtgcctt tgatctcttc
     3001 cacgaacgct ccgctcacaa gcgccgggat cacgggctcc aaacgggcaa atggagcgcg
     3061 caattaa
//
LOCUS       DAOOVC010000284         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_17507_length_3031_cov_4.778226, whole genome shotgun sequence.
ACCESSION   DAOOVC010000284 DAOOVC010000000
VERSION     DAOOVC010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_3031_cov_4.778226"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1163
                     /locus_tag="PLM79_18450"
     CDS             240..1163
                     /locus_tag="PLM79_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88343.1"
                     /translation="MPWTHVLCPDGQKKSFEECLACLHECFDLEIREAMFERERNWQA
                     IEHTGERISVTALLGCLRSTYLERVTDYAAHPESQWWSLRGELIHRLVERPDMDNPYL
                     RRSELRLRAKVNGYEVSGQLDNYKLRFLEMGILKDWKSIGDAGLQYIVFDGAKEDHVW
                     QCNIYAWLGRQNGYRIDRIEIAYLSLMQVVKTGKVVPFTEFLVSPPARSGKRKNMIGT
                     PRLIREYTSGKRKWECFYRIPEVPIYDDETVTGFITPRLACLTQAFEAGIMPPVAEDE
                     IRRWKCDGYCRVRDTCEAEERKNGRSLIIPH"
     gene            1182..2015
                     /locus_tag="PLM79_18455"
     CDS             1182..2015
                     /locus_tag="PLM79_18455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88344.1"
                     /translation="MELKTLTGKDLYESFRELDRQLPQHAYKKIEGGKGGKLGLTDII
                     PAFLPDLLLELFGPIGYGWGFTLHSMESSSKTVERAKGYTEEEVTATCQLSIWYRFLC
                     DGEPVMSDPVPATGGSTNTLVEWAMKGALTNALGCAWFFAGYQLSVYKNERSHNGNGE
                     SPGRSQGAQQRQRTQPGGRQQQPQRPVNAHDEWSAAMSSIEQLLSGLSLPKDRYWQYA
                     TGKYKTARDNITTEQLREQVALLTQCRQNQAKLEQFQKILTDIDSQKPAESGGQQGAM
                     H"
     gene            2028..2357
                     /locus_tag="PLM79_18460"
     CDS             2028..2357
                     /locus_tag="PLM79_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88345.1"
                     /translation="MMKKEQLIIHHSAFIIPVGATMRQKHMFFDAEEYWRDKKYKCWV
                     VCLSSRKGKVKPSTSGRRKEYRAYYNVQARTAERAIEVAKRHCTVLSGRIEGTARLAM
                     PRDLGAC"
BASE COUNT          764 a          834 c          791 g          642 t
ORIGIN      
        1 ggccaccgag actcagatcc cggatgactt ctacgctcca gagcagcccg cagaacagat
       61 gctgatgtag tgaacagtga gcagtgaacg gtgaacagga aaaacatttc accctgtctc
      121 actgtctatc tgctttcttt tgttcactgc tcactgttct ctgagatggg gcggggcgcc
      181 ggccaccacg gaaatcccac caaccagttt cagcttgaat ccagctaagg agtaaggtca
      241 tgccttggac acatgtactt tgcccagatg ggcaaaagaa gagctttgag gaatgtcttg
      301 cctgccttca cgaatgtttt gacctcgaga tccgtgaagc gatgtttgag agggaacgta
      361 actggcaggc catcgagcac accggtgaga ggatcagcgt taccgcgctt ctcggatgcc
      421 ttcgcagcac gtatctcgag agagtgaccg actacgccgc tcatcccgag tctcaatggt
      481 ggtcgttgcg cggagagctc atccaccgcc tggtcgagcg cccggacatg gacaacccct
      541 atcttcgccg gtcggaattg cgcctcagag ccaaggtcaa cggctacgag gtaagcggcc
      601 agctcgacaa ttacaagctg cgtttcctgg aaatgggaat cctcaaggac tggaaaagca
      661 ttggggacgc gggtttgcag tacatcgtct ttgacggcgc caaagaagat cacgtgtggc
      721 agtgtaatat ctatgcctgg cttggccgtc agaacggata ccggatcgat cgcatcgaga
      781 tcgcctacct ctccctcatg caggttgtga aaaccggaaa ggtcgttcct ttcaccgagt
      841 ttctcgtgag ccctccagcc aggagcggca agcgcaagaa catgatcggg actccccggc
      901 tcattcgtga gtacacttcg ggcaaaagga aatgggagtg cttctaccgc attcccgaag
      961 tccccatcta cgacgacgaa accgtcaccg gcttcatcac accaagactt gcctgcctca
     1021 cccaggcctt cgaagccgga atcatgcctc ccgttgcaga ggacgaaatc cgccgctgga
     1081 agtgcgacgg ctactgcaga gtccgtgaca cctgcgaggc cgaagaaagg aaaaacggcc
     1141 gatccttgat cattcctcac tgagagaaag gagatgtttt catggagctt aaaactctga
     1201 caggaaaaga tctatacgaa tccttccgag agctcgacag acagctccct cagcatgcct
     1261 acaaaaagat cgagggcggc aagggcggaa agctgggcct gaccgatatc atcccggcct
     1321 tccttcccga tcttctcctc gagctctttg gccccatcgg ctacgggtgg ggctttactc
     1381 tccacagtat ggaatcctca tccaaaacgg ttgaacgagc caagggctac acggaagaag
     1441 aagtcaccgc tacctgccag ctctcgatct ggtaccgttt cctgtgtgat ggtgagccgg
     1501 tgatgtcgga tcctgtgccg gccacaggcg gcagcacgaa cacgctcgtt gagtgggcga
     1561 tgaagggcgc cctcaccaac gctttggggt gcgcctggtt ctttgcaggc taccagctca
     1621 gcgtctacaa gaacgaaaga tcccacaacg gcaacggtga gagcccagga aggagccagg
     1681 gtgctcaaca gcgccagaga acccaacccg gaggcagaca gcaacaaccg caaaggccgg
     1741 tgaatgccca cgacgagtgg agtgctgcta tgagctctat tgaacaactg ctctcgggtc
     1801 tgagccttcc caaagacaga tactggcagt acgccacagg aaagtacaag accgcgcgcg
     1861 ataacatcac cacggaacag ctccgggagc aggtcgccct actcacccag tgtcggcaga
     1921 accaggcaaa gctcgagcaa tttcagaaga tcctcaccga catcgacagc cagaagccgg
     1981 cggaatcggg cggtcaacaa ggcgcaatgc actgatcgaa atgatgaatg atgaagaagg
     2041 aacaactcat cattcatcat tccgcattca tcattccagt gggggctacc atgaggcaga
     2101 agcacatgtt cttcgatgcg gaagaatact ggcgggacaa gaaatacaaa tgttgggtgg
     2161 tttgcctctc atcgagaaaa ggaaaggtaa aaccctcaac atctggacga aggaaggagt
     2221 accgcgccta ctacaacgtt caggccagga ctgcagagcg ggccattgaa gtcgccaaac
     2281 gccactgcac ggtgctcagc ggaagaattg agggaactgc acgtctggct atgcctcgag
     2341 atctcggcgc atgctgaatc tcggatcagg ataaaacaaa gaaatccgag ttccaggttg
     2401 ggttttattc ctcctcctga gctataccct tatcctgacg caggttgatt ttcgctatta
     2461 ctggctactc ttatgggaaa ccatgctgtc cttcgagaag gtctttttca cccctgcctg
     2521 gagcacaaag aacaatatgc ttcgtatcag tatcagccga gccacgaaat tcaattccct
     2581 ccctccggcc cgtccacaac aatctcagat actcatgaaa ccttctcccc gataggcacc
     2641 tcgattcccg cctttgaaac ttccgtcatc agagataact cccatgttgt ccaccattgg
     2701 ttttctcctg ggtggatgag cagccaagct gagatcctta ttgggcttgg acgtcttcag
     2761 ccggtcccag aatttccgct tcgacatttc cgaggtcctg ccaaatggca cgagaaccac
     2821 tgttctcctt gaggaaaagc gttgctgacc ctgctaacgt cccgaatgag tgctcaatcc
     2881 tcttaatcct ctagtgatca caaatgaaat ctgtgtaatt ggttcgttga tgtactctct
     2941 tgagggcgca tgggtaaaaa gggggtcaag gcatcttttt aatgtaaaat cagtgagtta
     3001 gcgatgatat ttgtagtggt ttaatgtaag c
//
LOCUS       DAOOVC010000285         3021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_17577_length_3021_cov_7.250843, whole genome shotgun sequence.
ACCESSION   DAOOVC010000285 DAOOVC010000000
VERSION     DAOOVC010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17577_length_3021_cov_7.250843"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PLM79_18465"
     CDS             <1..714
                     /locus_tag="PLM79_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88346.1"
                     /translation="QEACDILGVSDLRDYFRQPAGFFQDHLKRYSKSRRKAPIYWPLS
                     TASGSYTIWLYYHRLNDQTLYMVVNKYVEPKIAEVQKAVDSMQYAVEARERGITDTLL
                     TAHCPLPTGQLRQQLEDARAFLGELRDLREELLRIAALPYKPDLNDGVIINAAPLHRL
                     FRLRSWAKDTEDCWKKLEKGDYDWAHLAYTIWPERVREVCKRDRSIAIAHGLEDLCEA
                     QSPEPKKKGGRGRRKTSGA"
     gene            730..951
                     /locus_tag="PLM79_18470"
     CDS             730..951
                     /locus_tag="PLM79_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88347.1"
                     /translation="MKITNNPFNNPPGERIEFVAPTPLGRRLLSLRKEAVAEGMSLLT
                     EEEVLDEVGNRRSGGALRCCRAETGGSDV"
     gene            944..2092
                     /locus_tag="PLM79_18475"
     CDS             944..2092
                     /locus_tag="PLM79_18475"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88348.1"
                     /translation="MYKINRIQITGFRRIMNLNLDIRPFMVLIGANGVGKTSFLDALS
                     ILSASASGNLNAALSQFGGVANLLTRGKSDGLSFLVDMTVPDHEPLEYELRLEPKGTG
                     YSIARELLCQRRSGYHEPFKHIDSSDGDIKYYEIETNRLVRPDWEHNPLETSLSQVPK
                     MFRQPEELRRILATATQYHVLDVGPRAPVKMPQPMKPATLPGHDGADLVPYLYTLREA
                     ERDRYEAVLDALNAAFPDFEELNFPPVAAGMLTMTWKDKKFSKPIYMNELSEGTLRFL
                     WLTALLQSPHLSTITMIDEPEVSLHPELLSLLADLMREAAKRTQLVVATHSDRFIRFL
                     KPEEVVVMDLDDEGCASATWADSMDIEHWLAEYSLDEVWRMGRMGGRP"
     gene            2089..2703
                     /locus_tag="PLM79_18480"
     CDS             2089..2703
                     /locus_tag="PLM79_18480"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOV88349.1"
                     /translation="MKIALIVEGKTEKAFLPSLHKFLQGRLAGAMPRLDVSPYDGRIP
                     KEGKLKRVLENLLAGRNPADHVIALTDVYTGTNPPDFIDAQDAKDKMRRWVGEEPRFH
                     PHAAQYDFEAWLLPYWPTIQKLAKHNKAAPGGDPETVNHDNPPAYRIKEIFETGKCRD
                     SYVKPRDAARILREKDLSTAINQCSELKAFINTILSVCGGPVIP"
     gene            2765..>3021
                     /locus_tag="PLM79_18485"
     CDS             2765..>3021
                     /locus_tag="PLM79_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88350.1"
                     /translation="MKLTSFLHKKLVDLLGERRIVVWYDVEGDFADFVRSGRWAVGSG
                     QQTVVNDLSLPNTVVIDTRESVLKARRRADEIYRLMNESENP"
BASE COUNT          700 a          878 c          860 g          583 t
ORIGIN      
        1 caggaagcct gcgacatcct cggtgtctcc gacctgcggg attacttccg ccagcctgcg
       61 ggcttcttcc aggaccacct gaagcgctat tccaagagcc ggcgcaaggc cccgatctat
      121 tggccgcttt ccaccgcttc gggatcgtac accatctggc tctactacca ccgcttgaac
      181 gaccagacgc tctacatggt cgtcaacaag tatgttgaac ccaagatcgc cgaggtgcag
      241 aaggcggtag acagtatgca gtatgcagtc gaggcacggg aacgaggcat cacggacacc
      301 ctgctcactg cccactgccc actgcccact ggacaactgc gtcagcagtt ggaggacgcc
      361 cgcgcgttcc tcggcgagct gcgggacctg cgcgaggaat tgctccgcat cgccgcgctg
      421 ccctacaagc cggacctcaa cgacggcgtg atcatcaacg ccgcgccgct gcataggctt
      481 ttccgcctgc gctcatgggc caaggacacc gaggattgct ggaaaaagct tgagaaaggc
      541 gactacgact gggcgcacct ggcatacacg atatggcccg agcgtgtccg ggaagtctgc
      601 aaacgcgacc gctccatcgc aatcgcccac ggactagagg acctctgcga ggcccaatcc
      661 ccggaaccca agaagaaagg cggccgcggc cgtcggaaaa catccggcgc ctgagcaaag
      721 gagaaactaa tgaaaattac gaacaatcct ttcaacaatc cgcccgggga gagaatcgag
      781 tttgtcgctc cgacgccttt gggccgaagg cttttatcct tgaggaagga agccgtggcc
      841 gagggaatgt cgctacttac cgaagaagag gttctcgatg aggtcggtaa cagaagaagc
      901 ggcggagcgc ttcgatgttg cagagcggaa acgggaggtt ccgatgtata aaatcaaccg
      961 aatacaaata accggattca ggcggatcat gaatctgaac ttggacatcc ggccgttcat
     1021 ggtgctcatc ggcgccaacg gggtcggcaa gacttctttt ctggatgccc tctccatcct
     1081 gtccgcctcg gcctcaggca atctgaatgc cgccctgtcg caattcggtg gggttgccaa
     1141 tctcttgacc cgcgggaaaa gcgatggttt gtcgttcctc gttgatatga cggtgcccga
     1201 tcacgagcct cttgaatatg aactgcggct tgaacccaaa ggaacggggt attccattgc
     1261 cagagaattg ttgtgtcagc ggcgttctgg ttaccacgag ccattcaagc acatcgattc
     1321 gtccgacggc gacatcaagt actacgaaat agagaccaat cgtctcgtcc gccccgattg
     1381 ggagcacaac cccctggaga catcgctctc ccaggtgccg aaaatgtttc gccagccgga
     1441 agaactgcgg cgcattctgg cgaccgccac ccagtatcat gttttggacg tcggcccccg
     1501 tgcgccggtg aaaatgccgc aacccatgaa gcccgcgacc ttgccgggac acgatggcgc
     1561 cgacctggtg ccgtatctgt acacgttgcg tgaggcggag cgggatcgct atgaggccgt
     1621 tctggatgcg ttgaatgcgg ccttcccgga tttcgaggag ctgaattttc cgccggtggc
     1681 ggccggaatg ctgaccatga cctggaaaga taaaaagttt agcaagccga tctacatgaa
     1741 cgaactctcg gaaggcaccc tgcggttcct ctggctcacg gcgctgctgc aaagcccgca
     1801 tctttccacc atcaccatga tcgacgagcc ggaggtcagc ctgcatccgg aattgctgag
     1861 ccttctggcg gatttgatgc gcgaggccgc caagcgcaca caactggtgg tcgcgaccca
     1921 ttcggacagg ttcatacgct tcctcaaacc ggaagaagtg gtggtaatgg acttggacga
     1981 cgaaggttgc gcatcggcga catgggcgga ctccatggat attgagcatt ggttggccga
     2041 atacagcctc gacgaagtat ggcgcatggg ccggatggga ggtcggccat gaaaatagcc
     2101 ctcatcgtcg aaggcaagac cgaaaaggcg tttcttcctt ctctgcacaa atttttgcaa
     2161 ggccgcctgg ccggcgccat gcccaggttg gacgtctccc cctacgacgg ccgcatcccc
     2221 aaagaaggca aactcaaacg ggttttagag aaccttttgg caggaagaaa ccctgcggac
     2281 catgtgattg cgctgaccga cgtgtacacc gggacaaatc ccccggactt catcgatgcg
     2341 caagacgcca aggacaaaat gaggcgatgg gtgggagagg aaccgagatt tcatcctcat
     2401 gccgcccagt atgatttcga agcatggctt ttgccttact ggccgacgat ccaaaaattg
     2461 gcgaaacaca acaaagccgc ccccggcggc gatccggaaa ccgtgaatca tgacaatcca
     2521 cccgcatacc gaatcaagga aatctttgag accggcaagt gccgggacag ctatgtcaag
     2581 ccccgcgatg cagcgcggat cctcagagaa aaagatcttt cgacagccat caatcaatgc
     2641 tcggaactga aagcctttat caacacaatt ctctcagtgt gcggaggccc cgtcatccca
     2701 tgatcctgat cggatgtttt attcctgccc acattctact gcccaccggc aaagggggtg
     2761 cccgatgaag ctcacctcgt tcctgcacaa gaagctggtc gacctcctgg gagagcgtcg
     2821 catagtggtc tggtatgacg tcgaagggga ttttgcggac ttcgttcgca gtgggcggtg
     2881 ggcagtgggc agtgggcagc aaaccgttgt gaatgacctg tctttgccaa atactgtggt
     2941 catagacacg cgagagtccg tgctgaaagc gcgccgccgg gccgacgaga tttaccggct
     3001 gatgaacgaa tccgagaacc c
//
LOCUS       DAOOVC010000286         3015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_17621_length_3015_cov_6.466216, whole genome shotgun sequence.
ACCESSION   DAOOVC010000286 DAOOVC010000000
VERSION     DAOOVC010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17621_length_3015_cov_6.466216"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..723)
                     /locus_tag="PLM79_18490"
     CDS             complement(91..723)
                     /locus_tag="PLM79_18490"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV88351.1"
                     /translation="MIVGRLPYILFMGFFLLFAGFSATDPGLCAPGPLAVIQSGTDKA
                     LEILHSSQSGQAPALRARRGEILRIVDEYFNFHEMAKRALGRPWKDQSPQNQKEFVDL
                     FKQLLFNSYVDKVEAYTGSNERVVYDAEKVEGEYALVKTRVLGYKNADVQVDYRLRAE
                     EGIWKVYDVVVEGISLVNNYREQFSSILANESFDSLLKRMRGKLDEQSMA"
     regulatory      900..1084
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1381..1542
                     /locus_tag="PLM79_18495"
     CDS             1381..1542
                     /locus_tag="PLM79_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88352.1"
                     /translation="MKKQVLCKQCGRPFVKYAYHRTARCEECRKADPAAHARSATIAL
                     TRPGETDKS"
     gene            1603..2523
                     /gene="fabD"
                     /locus_tag="PLM79_18500"
     CDS             1603..2523
                     /gene="fabD"
                     /locus_tag="PLM79_18500"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOV88353.1"
                     /translation="MREWVFLFPGQGSQYVGMGRDFYENYAEVRELFSIAGDLLEMDV
                     AKLCFEGPEEMLVLTENVQPAITVVNLACLTVLHLHEIYPAAAAGHSLGEYSALYAAG
                     VLGLRDVLKLVRWRGKLMHEASLQEPGSMAAIIDLTPDKVREICVACDVEVANINCPD
                     QIVITGPHELVERAIALGTEVGAQKCIKLNVSGPWHSRCMAYARERFEPVVRECTFRE
                     PQIPILHNVDSLPLQAAKDAPERLIEQICRPVLWKQSMERLVSFGRRNFVEVGPKKVL
                     RGLMRRIDRTVKVLNVEDSASLASFLQANQ"
     gene            complement(2533..>3015)
                     /locus_tag="PLM79_18505"
     CDS             complement(2533..>3015)
                     /locus_tag="PLM79_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOV88354.1"
                     /translation="AQAFKKACIDASLYAVLAICALLLFLFRHVGYTLLAWIPLAVGM
                     LWTVGIMGWWGTDFNLANSIFMPLVVGAGVEYGIIILQRWREGKILPGHLPLSTGKGI
                     ILAALTTTVGFGSLMISDHQGIFSLGFVAAVGSLCVLVAAVVVVPAVLALMPRPDPSS
                     "
BASE COUNT          642 a          882 c          926 g          565 t
ORIGIN      
        1 ctccgggagg ggcgtccgac cggggcagaa tgcctgcccg gaaggtgcat gtctcgtcct
       61 ccgggacaaa ccgcaggccg ctgggcggcc ctaggccatg gattgctcat ccagcttccc
      121 gcgcatacgc ttgagaagac tgtcgaagga ttcgttggcg aggatggagc taaactgctc
      181 gcggtagttg ttgaccaggc tgatgccttc gacgaccacg tcgtacactt tccagatccc
      241 ttcctcggct ctcaggcgat agtccacctg gacatcggcg ttcttgtatc ccaagacccg
      301 ggttttcacc agggcatact ccccttccac cttctcggcg tcgtagacga ctctttcgtt
      361 ggagccggta taggcttcca ccttgtccac gtaggagttg aagaggagct gtttgaacag
      421 gtccacaaat tccttctggt tttgcggcga ctgatctttc cagggcctgc ccagcgcgcg
      481 cttggccatc tcgtggaaat tgaagtactc gtcgacgatt ctcaggatct cgcctcttct
      541 tgccctcaag gcgggggcct gtccggactg ggagctgtgg agaatctcca gggccttgtc
      601 cgtcccggac tgaatgacgg ccagcggtcc tggggcgcag agccctggat ccgtggcgga
      661 gaaccccgcg aagaggagaa agaatcccat gaaaaggatg tacggtagtc gtccgacaat
      721 catcacgttc actcccgttg aatcacagcc caaaacacgc ccggtaaacg ggtctcgaat
      781 gcagtattct agcaccaatc gccctcgaag ggagaacttt ttgcccgcag tccctcccat
      841 ccttgcccct gggaatagtc cttgacgagg cggcggcggt ccgatagagg tatccgtact
      901 tttatggatc ggcaccaggt accgaagagt ttaaagggga atcccgtgaa agtcgggaac
      961 ggtcccgccg ctgtgaacgg ggacgaaatc cgctgaagcc actgccctgc gctgcagggt
     1021 gggaaggttc ggagagtagg gcgatccgtg agtcagaaca cctgcctgct gccgcacggg
     1081 ggatggatac gaggagctgt tcgaccccct ccggacggat caaggaaatc agggtgcaat
     1141 cctcaaagct tcgagctttg gggatttttt ttgcccgggt ctcttcctgc atccgggaat
     1201 caaccggaaa aaaaggaatt tcatggtctg tggaaggacg gaccgggtcc cgcgtggcgg
     1261 gtgtccggcc ggatcttttc gggatggttg aggaagagct cgaaggaagg agcccgacaa
     1321 aggcgggctc gacccatcgg agcactttga cccaatggcg aaacacgggg gtaccgacag
     1381 atgaaaaagc aggtcctgtg caagcagtgc ggaagaccct tcgtcaagta tgcttaccat
     1441 cggacggcgc gctgcgaaga gtgccggaaa gcggaccctg ccgcccacgc ccggtcggcg
     1501 acaattgcct tgacaaggcc gggggagacc gataaaagct agcccaaatt tgcggacccg
     1561 gaaaacgatc tcaccccgtc cccagctgca aggagcttgc gcatgagaga gtgggttttc
     1621 ctctttcccg gtcagggatc ccaatacgtc ggaatgggaa gggattttta cgagaactat
     1681 gccgaggtga gggagctgtt ctccatcgcc ggggaccttc tcgagatgga cgtggccaaa
     1741 ctgtgtttcg agggaccgga agagatgctg gtcctgacgg aaaacgttca gcccgccatc
     1801 acggtggtca acctggcctg cctcaccgtt ttgcaccttc atgaaatcta cccggcggcg
     1861 gcggcgggcc acagtctcgg ggaatacagc gctttgtacg cagcgggggt gttgggcctc
     1921 cgggatgttc tgaaactggt gcggtggaga ggaaagctca tgcatgaggc ttctcttcag
     1981 gaaccggggt ccatggcggc catcatcgac ctgaccccgg acaaggtccg ggagatctgc
     2041 gttgcctgtg acgtcgaagt ggccaacatc aattgcccgg accaaatcgt aatcaccggg
     2101 ccccacgagc tggtggaaag ggccatcgcc ctgggcaccg aggtgggggc ccagaaatgc
     2161 atcaagctca atgtcagcgg accctggcac agccggtgca tggcctacgc ccgggagcgc
     2221 ttcgagcccg tcgtgcggga atgcacgttt cgggagccgc agattcccat cctccacaac
     2281 gtggattccc tgcccctgca ggcggccaaa gacgcaccgg aacggctcat cgagcagatc
     2341 tgccgccccg tattgtggaa acagtccatg gagcgcctgg tgagcttcgg tcgtcggaat
     2401 ttcgtggaag tgggtcccaa gaaggtcttg aggggcctga tgcgacggat cgaccgaacc
     2461 gtcaaggtcc tcaacgtgga ggattccgcc agcctggcaa gctttctaca ggccaatcaa
     2521 tgaatcgaaa cgtcaggagg aaggatctgg tcggggcatg agagccaaca cggcgggcac
     2581 caccacgacc gcggccacca gaacgcacag gcttcccacc gccgccacaa aacccaggct
     2641 gaaaattccc tggtgatcgg agatcatgag gctcccgaag cccacggtgg tggtcagggc
     2701 ggccaggatg atcccctttc ccgtgctcaa cggcagatgc cccgggagaa ttttcccttc
     2761 ccgccatcgc tgcaggatga tgattccgta ttcgaccccg gcccccacca ccaggggcat
     2821 gaagatgctg ttggccaggt tgaaatccgt tccccaccag cccatgatcc ccaccgtcca
     2881 aagcatcccg acggcgaggg gaatccaggc caggagcgta tatcccacgt gtcggaaaag
     2941 gaacaacagc agggcgcaaa tggccagcac ggcatacagg gacgcatcga tgcaggcctt
     3001 cttgaaggcc tgggc
//
LOCUS       DAOOVC010000287         2938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_18222_length_2938_cov_3.920569, whole genome shotgun sequence.
ACCESSION   DAOOVC010000287 DAOOVC010000000
VERSION     DAOOVC010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18222_length_2938_cov_3.920569"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PLM79_18510"
     CDS             complement(<1..1321)
                     /locus_tag="PLM79_18510"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOV88355.1"
                     /translation="MQQDKPLKIIRLIAENVKKLRAIQITPEGHIVQISGPNAAGKTS
                     VLDSIWYGLGGGQALPEEPIRKGAARGEIHLDLGEMIITRTFTQKGSYLKVVSKDGLE
                     WKSPQKLLDKLIGKFSFDPLAFARLDESKQRELLLSLVTIPLSRDHLQHISGMPVQRN
                     GDPINSMNTVYKALYDQRADHNRNVKRIEAAIASIAIPQGMEDVEPISAGRLVAERRE
                     LEAANARNEAARRQFLDLEQQNKALKDQKEQLESRKQDLLKQVQEVEEAIHETESLIE
                     ASDAELVQAANATRDLVDHDFSEIDTRIVQADEVNRIAGEVARRKALQEELDREQKQA
                     SECTAKLEAIGRYKTQLMEQTNFPIGGLDFRAGKVVYNGVPIGQASAAEKLRVGMAIA
                     MALNPRLRIIRIEDGSLLDKQSWRVIEEMAQQGDYQVWIETVADEPGQ"
     gene            complement(1447..2385)
                     /locus_tag="PLM79_18515"
     CDS             complement(1447..2385)
                     /locus_tag="PLM79_18515"
                     /inference="COORDINATES: protein motif:HMM:NF022792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3150 domain-containing protein"
                     /protein_id="HOV88356.1"
                     /translation="MNSNVFQEACLLQLNTSCWQGSRMLDPAVMERIGNSEWLRGRKH
                     LISPEALNPIRAAISRARKDVERFALPFPLVGLTLVPKEQLARIEECLATHRADYWNE
                     VEKFEDQYEEAREAARLNLGEFFSEADYPINIRSRFGFEWRYFVLDVPGKAGILTPEI
                     YEREKAKFMAMMEETRELATAALRQEFADHVSHIVERLTRSEDGKPKTFRNCMVEKIQ
                     SYLDEFGGRNLFEDDHLAELVGKAKAIIGGVSPESIRENIWLKNSIAEGMGKIKQAID
                     HAIIDLPRRKVRLSDTPSAHPAISTEAASSMAAAAP"
     gene            complement(2442..2645)
                     /locus_tag="PLM79_18520"
     CDS             complement(2442..2645)
                     /locus_tag="PLM79_18520"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOV88357.1"
                     /translation="MKQIIIDITNEGEVKIETRGFRGKACLAESQFLKDVLGEETARQ
                     LTPAYYQRQDSKQVVKRHLPLCG"
     gene            complement(2642..>2938)
                     /locus_tag="PLM79_18525"
     CDS             complement(2642..>2938)
                     /locus_tag="PLM79_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88358.1"
                     /translation="GAEDRTGNSPDPDEVRCPMHEIFKGTTPYSAESTEQQMNIIPIR
                     MALFSPQFWNLWDQLMDRERAAYTLGWIAELEMAIRRMSEQLRRIEDHLEEDGT"
BASE COUNT          536 a          821 c          816 g          765 t
ORIGIN      
        1 cctggccggg ctcatcggca acggtttcga tccatacctg gtaatcgccc tgctgggcca
       61 tctcttcgat gactctccag gactgcttgt cgagcaaaga tccgtcttca atgcggatga
      121 tccggagcct cgggttgagg gccatagcta tggccatacc aacgcgcagc ttctcggctg
      181 cgctcgcctg gccgatgggg actccgttgt agacgacttt tcctgcgcgg aaatcaaggc
      241 ctccgatcgg gaagttggtc tgctccatga gctgagtctt gtatcggcca atggcctcga
      301 gctttgcggt gcactcgctc gcctgttttt gctcccgatc gagctcttcc tggagcgctt
      361 tcctcctggc aacctcgccg gcgatgcggt tcacctcgtc ggcttggacg atccgggtat
      421 cgatctcgga aaaatcatgg tccacaagat ccctggtcgc gtttgccgcc tgaacaagct
      481 ctgcatccga agcctcgatg agagactcag tctcatggat ggcttcttca acctcctgga
      541 cctgcttcag cagatcttgt ttccgggatt cgagctgctc tttctggtct ttcaatgcct
      601 tattctgctg ctccaaatcg aggaattgcc gtcgggctgc ttcattacga gcattggcag
      661 cttcgagctc acgcctttca gcaaccagcc ggccggcact gatgggctct acgtcttcca
      721 ttccttgtgg aattgcgatg gaggcaatgg ccgcctcgat tctcttgaca tttcggttgt
      781 ggtccgctcg ctggtcatag agagccttgt agacggtgtt catggaattg atcggatcgc
      841 cgtttcgctg cacgggcatg ccggatatgt gctggagatg atctcgggag agcgggatgg
      901 taacgaggga aagcaggagc tcgcgttgct tggattcatc aagcctggcg aaggcaagag
      961 gatcgaagga gaacttcccg atgagcttat cgaggagctt ctgcgggctt ttccactcga
     1021 gtccatcttt gctgaccacc ttcaagtagg atcccttctg agtgaaggtc ctggtgatga
     1081 tcatctcccc gaggtcgagg tgaatctcgc ctcttgccgc tccctttcgg atgggctcct
     1141 ccggaagcgc ctggccgcct ccgagtccat accagatgga atccagcacg ctggttttgc
     1201 ccgcagcatt ggggccgctt atctgtacga tgtgcccctc tggtgtgatc tggatggccc
     1261 ggagcttctt cacgttctcg gctatcagcc ggatgatctt tagcggctta tcctgttgca
     1321 tggtttgcct cctttttgga atgatgaatg cggaatgatg agtgatgagt ggacgattga
     1381 tttcattcat cattcgtcac tcatcattag tgacaggcag gaacccgacc aaaggggcta
     1441 ccggatctac ggagctgcgg ctgccatact cgaagcagct tctgtggaaa tggcgggatg
     1501 cgctgagggg gtatcggata gacgaacctt gcgccgcgga agatcgatga tcgcatggtc
     1561 aatggcctgc ttgattttgc ccatgccttc agcgatgctg ttcttgagcc agatgttttc
     1621 ccggatcgac tcggggctta ctccgccgat gatggccttt gctttcccga cgagttctgc
     1681 aaggtgatcg tcttcaaaga ggtttctccc tccgaactca tcgagatagc tctggatctt
     1741 ttccaccatg cagtttctga acgtcttggg cttgccgtcc tcgctcctgg taagacgctc
     1801 aacgatatgg ctcacatgat cggcgaattc ctggcgcagg gcggccgtag cgagctctct
     1861 ggtttcttcc atcatggcca tgaatttggc cttctcgcgc tcgtatatct ccggagtgag
     1921 aatgccagcc ttgcctggaa catcgagaac aaaatagcgc cactcaaagc cgaacctgct
     1981 tcggatattg atcgggtaat cggcctcgct gaagaactcc ccaaggttca gccgggctgc
     2041 ctcgcgggcc tcctcatact gatcctcgaa tttttcgact tcattccagt aatccgcgcg
     2101 gtgcgtggca aggcactcct cgatcctggc gagctgctct ttgggaacga gggtcagccc
     2161 aacgagtgga aacggcaagg caaatcgctc gacatccttt cgagcgcggc ttattgcggc
     2221 tcggatgggg ttcagcgctt ccgggctgat gaggtgcttc cgccccctca accattcgct
     2281 gtttccgatt ctctccatca cggctggatc gagcatgcgg gacccctgcc agcatgatgt
     2341 attgagctgc aggagacagg cctcctggaa gacgttgctg ttcatggcat actccttctg
     2401 tctaggatgt ggtcttcaac aaggatggat gggactgcgg gttagccaca aagtgggagg
     2461 tgtcttttca cgacctgttt cgaatcctgg cgctggtagt aggcgggggt gagctgcctt
     2521 gcggtttcct ctccaagaac atccttgaga aactgagact cggctagaca ggcctttccc
     2581 ctgaaaccac gggtctctat cttcacttcc ccttcattgg ttatgtcgat gattatctgt
     2641 ttcatgtgcc gtcctcctcc aggtgatcct cgattctgcg aagttgctcc gacattctgc
     2701 ggatggccat ctcaagctct gcaatccatc cgagggtgta ggcagccctc tcgcggtcca
     2761 tcaactgatc ccagagattc cagaactgcg ggctgaagag ggccatacga attgggatga
     2821 tattcatttg ctgttcggtc gattcggcgc tgtacggggt ggttcctttg aaaatctcat
     2881 gcatggggca cctcacttcg tcaggatcag gcgaattccc tgttcggtct tccgctcc
//
LOCUS       DAOOVC010000288         2873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_18779_length_2873_cov_3.357346, whole genome shotgun sequence.
ACCESSION   DAOOVC010000288 DAOOVC010000000
VERSION     DAOOVC010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18779_length_2873_cov_3.357346"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="PLM79_18530"
     CDS             <1..277
                     /locus_tag="PLM79_18530"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOV88359.1"
                     /translation="DILNGKGVRVPLVIHGGTGLSYEVTRRLVSLGGAKFNVSTELKY
                     ALIDASYEYLAANKNDYNPGKLDAAVYTRTVALIEKWIDVLGSAGKY"
     gene            297..1046
                     /locus_tag="PLM79_18535"
     CDS             297..1046
                     /locus_tag="PLM79_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOV88360.1"
                     /translation="MGVIQAVLFDQDGVIIDTERDGHRLAFNKAFRDFGFDVEWDVEF
                     YHKLLQVGGGKERIKYYFEKYYTGKKPEDMDGLIKKLHEAKTAAFIEILETMPLRPGI
                     HRFMREIKDRGLKIGICTTSNEKVAHTVAEKMLSDISFDVLIAGDMVSRKKPDPEIYL
                     SALKKIGVAPENCLVVEDSSIGVQAANAAGCRVIATVNGYTKDEDLSGADLVISCLGD
                     EQGEKAVFLAGSIPLRKAGIVSLEDIERALA"
     gene            1043..2362
                     /locus_tag="PLM79_18540"
     CDS             1043..2362
                     /locus_tag="PLM79_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOV88361.1"
                     /translation="MKILSVDFGTSSVKMAVLNEKEEILAETKVEYTYEVIDRLKIQI
                     DPEIVYSAFLKGLAAFQSHLKEIELLVPCVFAPVFIPMDKEGNALHPAIIHLDRRSYP
                     QSRFALKVVGKKTFLEISGNLPFAGGISCTSMLWYKDNCPELYARTYKLGHMNTFLQR
                     RLAGKWLIDPSNASFTGLYETRKMGGWSENICGALGIDRNLLPEIVPSMSFAGKLCRE
                     AALTTGLREGLPVVMGANDTTSAAFGAGAVNPGDILNISGSNEIITVTTDDPVPHEKY
                     YIRSSMEPGKWLFLAITVGGFALEWFRKEFYSEMDKGKFYGEYLTNFVKTHTRTDVKF
                     KPYLAGDRHSLGKKKGAFTGLTFESTREDLLLALLVGTYEPVFNTINILKKRMRLNPH
                     IFWTGGMVSEAYQDFKRRVFKGFDFNMRRECSTLGNLKVALILTGRK"
     gene            2379..>2873
                     /locus_tag="PLM79_18545"
     CDS             2379..>2873
                     /locus_tag="PLM79_18545"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOV88362.1"
                     /translation="MSSIKEKLEGVFPPVMTPFVGGKIDFRKLTANIEKMNPTRLRGY
                     MPLGSNGEFRSLTDDEALSVVKIIAGAKDPDKTLMVGTGRESAYATIEFTKRAADLGI
                     DFASVLTPHYYAKKMTDLALVRFFTEVADNSPVPILMYCAPGFAAGVVLSPRAVSELA
                     SHPNI"
BASE COUNT          699 a          742 c          822 g          610 t
ORIGIN      
        1 ggatatcctc aacggaaagg gggtacgggt ccccctcgtc atccacgggg gaacgggact
       61 ttcctacgaa gtgaccaggc ggctcgtttc tctgggggga gcgaagttca acgtatccac
      121 ggagctcaaa tatgctctta tcgacgccag ttacgagtat ctcgcggcca ataagaacga
      181 ttataacccc ggcaaactcg atgcagcggt atacaccagg acagtggctc tcatcgagaa
      241 atggatagac gttctgggtt ctgctggaaa atactgaaac ggggaggcgt aaaaggatgg
      301 gtgtgataca agcggtacta tttgatcagg acggagtcat tatcgatacg gaacgggacg
      361 gccatcggct ggccttcaac aaggcctttc gggacttcgg cttcgatgtg gagtgggacg
      421 tggagttcta ccataagctc ctgcaggtcg gcggaggcaa ggagaggatt aaatactatt
      481 tcgaaaagta ctacacaggc aaaaagccgg aggatatgga cggtcttata aagaagctgc
      541 atgaagccaa gacagcggcg ttcatcgaaa tcctggagac tatgcccctc cggccgggca
      601 ttcatcggtt catgcgggag attaaggacc gggggctcaa gatcgggatc tgtaccacgt
      661 cgaacgaaaa ggtcgcgcat acggtggcgg agaaaatgct ttccgacatc agcttcgatg
      721 tgctcatcgc cggggacatg gtgtccagga aaaagccgga cccggagatt tatctctctg
      781 ccctgaaaaa gatcggtgtc gcaccggaaa actgtctggt ggtggaggat tcgagcatcg
      841 gggtgcaggc ggcgaacgcg gcgggctgcc gggttatcgc aacggtcaac ggctacacaa
      901 aggacgagga cctctccgga gccgatctgg taataagctg cctcggcgac gagcaggggg
      961 aaaaggcagt cttccttgca gggagtattc ccctgcgtaa agccggtatt gtctccctgg
     1021 aagatattga gagggccttg gcatgaagat actctccgtc gacttcggaa cttcttcggt
     1081 caagatggct gtattgaacg agaaagaaga aattctcgca gagacgaagg tagaatacac
     1141 ctacgaggtc atcgatcggc tcaagattca gatagatccg gagatcgtat attccgcctt
     1201 tttgaaaggg ctcgctgctt ttcagagtca cctcaaggag atcgagcttc tggttccctg
     1261 cgtctttgct ccggtcttca ttcccatgga taaggaagga aatgccctgc acccggccat
     1321 cattcatctt gaccggagaa gttaccccca gtcccgcttc gcgctcaaag tggtggggaa
     1381 aaaaacgttc ctcgaaatca gcgggaacct gccctttgcc ggaggcatct cctgcaccag
     1441 catgctctgg tacaaggaca attgtccaga gttgtacgcc aggacttaca agctcggcca
     1501 tatgaatacc ttcctccaaa ggcggctcgc agggaagtgg ctgatagacc catcgaacgc
     1561 ctcctttacc ggtctatacg aaacacggaa gatggggggc tggtccgaga atatctgcgg
     1621 ggccctcggc atcgaccgga acctgctgcc ggaaattgtc ccttccatga gtttcgcggg
     1681 caaactctgt cgggaggcgg ctctgaccac gggtctcagg gaaggacttc ccgttgtcat
     1741 gggagccaac gataccacca gcgctgcctt cggagcaggg gcggttaatc ccggggacat
     1801 cctgaacatt tcgggaagca acgagatcat taccgttacc accgacgatc cagttcccca
     1861 tgaaaagtat tacatccgct cttccatgga gccgggaaag tggctcttcc tggccataac
     1921 cgtgggaggt ttcgccctgg aatggttccg taaggagttc tattccgaga tggacaaggg
     1981 gaaattctac ggcgaatacc ttacgaattt tgtcaaaacc cataccagga cagacgtcaa
     2041 attcaaaccc tacctggcgg gggaccggca cagcctgggg aaaaagaagg gtgcctttac
     2101 agggcttacc ttcgagagca cccgggaaga tctcctttta gccctgttgg tggggaccta
     2161 cgagccagtg ttcaacacca tcaatatcct gaaaaagcgg atgcgcttga atccccatat
     2221 cttctggacc ggtggcatgg tgagcgaagc ctatcaggat ttcaaacgcc gggtgttcaa
     2281 aggcttcgat tttaacatgc gccgggagtg ttccaccctg gggaatttga aggttgccct
     2341 gatcctgact ggaagaaaat aagaaacgga ggaatttcat gagttccata aaagaaaagt
     2401 tagagggcgt atttccgccc gtaatgaccc ccttcgtcgg ggggaagatc gattttcgaa
     2461 aactgacggc gaatatcgaa aagatgaatc ccacccggct ccggggctac atgcccctgg
     2521 gtagcaacgg cgaattcagg agtctcaccg atgacgaggc cctctcggtg gtgaagatca
     2581 tagccggggc aaaggacccg gacaagactc tgatggtagg aaccggtcgc gaatcggcct
     2641 atgccaccat tgagttcacc aagcgcgcag cggacctggg catcgatttc gccagcgttc
     2701 tcacccccca ttactatgcg aagaagatga ccgacctggc cctcgtgcgg ttttttaccg
     2761 aggtggccga caactctcct gtcccgattc ttatgtattg cgcaccgggt ttcgctgcgg
     2821 gggtagtgct ttctccccgg gcggtttccg aacttgcctc tcatccgaac atc
//
LOCUS       DAOOVC010000289         2869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_18812_length_2869_cov_4.432480, whole genome shotgun sequence.
ACCESSION   DAOOVC010000289 DAOOVC010000000
VERSION     DAOOVC010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18812_length_2869_cov_4.432480"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLM79_18550"
     CDS             <1..405
                     /locus_tag="PLM79_18550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88363.1"
                     /translation="PACGKIITKEEDGFEVPVTDLKDLADKRRFCTNQIPGWQLDNRQ
                     QRKLDDNGNPIWGMRKCGQALFEFTGARRHSIAEYIAKHARGAFKLLIADEVHQFQAK
                     ASDRGVAFHQLVTACKSTLTLTGTFFGGKSAS"
     gene            1024..2712
                     /gene="ltrA"
                     /locus_tag="PLM79_18555"
     CDS             1024..2712
                     /gene="ltrA"
                     /locus_tag="PLM79_18555"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOV88364.1"
                     /translation="MLDDILKTQHSFARKAVAEPEHRFKNLYHLLKRDDWLDVAMNEV
                     LSNVGARTGGVDGVTLKSFKQEGFREQFLNDLKRELSTGEYQPMPVKRKYITKSNGKL
                     RPLGIPTIRDRVVQMALKMLLEPIYESDFLDCSFGFRPGRRTMDAVVTFYRLINPRNS
                     YYWVIEGDIRSYFDKVNHDILLKLVAKRVADIKVLNLIGRFLKAGVMENQVFRHSPEG
                     TPQGGILSPLLANVYLHELDRWWMEKYGSLGRHAKEWRRKKGQSNCIYVRYADDWVAL
                     CNGTKAQAEAIRDEIRDFLRGTLKLELSEEKTVVTHACDGFDFLGFHIQHYPEHNGQK
                     AITLIRPSDKSINRLKDKVREMTSRKGFNDNPMFKFYALNAVLRGWMNYYRHCNASLI
                     ASWLDHWVHLKLAKWLVQRHESCYREILKKYLKQQGNRKNFAVKRTDGSDYFLFMMRD
                     VHITPHRLPKRSNPFLLQDPAGINVAEPEIPVPDDPWNGRAEPEYNTWRNLRDEVLER
                     DNYRCTICGGKENLDVHHIKPKRKDGQDVLENLVTLCEKCHVAQGGYGRPRKNE"
BASE COUNT          799 a          678 c          788 g          604 t
ORIGIN      
        1 ccggcatgtg gaaagatcat cacaaaggaa gaagatggct tcgaagtacc ggtcacagat
       61 ctgaaagacc tggccgataa acggcgcttc tgcaccaacc agatccctgg ctggcagttg
      121 gacaaccgcc agcaaaggaa attggatgac aatgggaatc cgatctgggg aatgcggaaa
      181 tgcgggcaag ccctgtttga gttcaccggc gcccgccgcc actcgattgc cgaatacatc
      241 gccaaacacg ccaggggagc tttcaagctg ctgatcgcag acgaggtcca tcagttccag
      301 gccaaggcct cggatcgagg cgtggctttt caccagctgg tcacggcctg taaaagcaca
      361 ttgaccctca ccggcacgtt cttcggcggc aagagtgcgt cttgaagttc ataacagtaa
      421 tgttcgaacg gctcatgaaa ggaggtgatg acaaaccgag ccagtagaac caccagcctc
      481 ccccaaggct gatgaagcta cccgtgcagc aggggaacat gaggaagaac ctgtgaaaga
      541 gctacgggga cataagcgga taagggctga accatctggc ccgatgaccg ccggcttgat
      601 cttgaccatg gctacgagag aaatcgttgt atgaagcatc caaagccgaa gacattccat
      661 taaggatggc tggccgagag gccgtggagt gagttgcagt taagtctcat ctttttgcat
      721 gaggcgaaaa ggttcttttt tcgaccgaac cgcaatgcta caggggtgta agctagcgga
      781 ctgcaacggg gacccagaga gggtcttgtg ggtgtagaga cggagcctaa agtcaaggta
      841 gatgtgctgt tatgggaaca agagagaaac aatgctcgac ccttcggggt tggactacct
      901 gcggttgatg tgagcattgt gagtcggagc cttcatagta gtctgaggac gggaaagccg
      961 tccacatggc gaagggaggc agctattcga agaaaactga tcgttgatga ggaggtccta
     1021 aaaatgttgg acgatatact caaaactcag cacagtttcg caaggaaagc cgtagcggaa
     1081 ccagaacacc ggttcaaaaa cctgtatcac cttctaaaaa gggacgattg gctggatgta
     1141 gcaatgaatg aggtgctatc caatgtcggt gctcgaacgg gaggggtaga tggagttacc
     1201 ctgaaaagct tcaagcaaga agggtttcgg gaacaattcc tgaacgacct caagcgagag
     1261 ctatccaccg gagaatacca acctatgcca gtcaaacgca agtacatcac gaaaagcaat
     1321 gggaaactgc gtcccttagg aatccccacc atccgagacc gggtggttca gatggccctc
     1381 aaaatgctgt tggaaccaat ttacgaaagc gacttcctgg attgctcctt tgggtttcga
     1441 cctggaaggc gcaccatgga tgcggtcgta acgttctacc ggctgatcaa tccacgcaac
     1501 tcgtactact gggtcattga gggtgatatt cgttcgtact tcgacaaggt gaaccacgat
     1561 atcttgttga aattggtagc caaacgagtg gccgatatca aagtcttgaa tctaatcggg
     1621 cggtttctga aagcaggtgt catggaaaac caggttttcc ggcactcgcc tgaagggaca
     1681 cctcaaggag gcatcctgtc acccttgtta gccaatgtct acctgcatga gcttgaccgc
     1741 tggtggatgg aaaagtatgg cagcctggga aggcacgcta aagaatggcg gcgcaagaag
     1801 ggacagagca attgtatcta tgtccggtat gccgatgatt gggtggctct atgcaatggt
     1861 acgaaagccc aagcggaagc gatccgggat gagatacgcg actttctgag aggtacccta
     1921 aagctggaac tgtctgaaga aaagacggta gttactcatg cttgcgacgg gtttgatttc
     1981 ctgggttttc atatccagca ctatcctgag cataatgggc aaaaggcaat cacgctaata
     2041 cgcccttctg acaagagtat caaccggctg aaggacaaag tccgagagat gaccagtcga
     2101 aaaggcttca atgataaccc gatgttcaag ttctacgcac tcaacgcggt gttacgggga
     2161 tggatgaact attaccgtca ttgcaatgcc agcttaatcg cgagctggct ggatcattgg
     2221 gtgcatctca agcttgcaaa gtggttggta caacgtcatg aatcctgcta tcgagagatt
     2281 ctcaagaaat atctcaagca gcaaggcaac cgaaagaatt ttgctgtcaa acgaaccgat
     2341 ggcagtgact actttctctt catgatgagg gatgttcaca tcacgcctca tcgcctgccc
     2401 aaacgttcca acccttttct gcttcaagac ccagcaggaa taaatgtggc agaaccagaa
     2461 attccagtgc cagacgaccc ctggaatgga cgcgccgaac cagaatacaa cacctggcgc
     2521 aacctccggg atgaagtctt ggaacgagat aattaccgat gcaccatctg cggtggaaag
     2581 gaaaatctgg atgttcacca catcaaacct aaacgcaaag acggccaaga cgttcttgaa
     2641 aacctcgtga cactttgtga aaagtgtcat gtagcacaag gtggctatgg cagaccacgc
     2701 aaaaacgaat aggggagagc cggatgatta cgaaagtatc atgtccggtt tggggagagg
     2761 agtggggaaa catgcctgtc gaaaggcagg agatgcgcct tgctcctact ctacgcacct
     2821 caattttctg gctgctgcat cgcttgaacc atggcgtccg tcgtgattt
//
LOCUS       DAOOVC010000290         2858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_18905_length_2858_cov_3.827328, whole genome shotgun sequence.
ACCESSION   DAOOVC010000290 DAOOVC010000000
VERSION     DAOOVC010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18905_length_2858_cov_3.827328"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLM79_18560"
     CDS             complement(<1..583)
                     /locus_tag="PLM79_18560"
                     /inference="COORDINATES: protein motif:HMM:NF019878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HOV88365.1"
                     /translation="MTLIELLNQDGTPTRRVSGTNGGEYAGPCPSCGGDDRFRVWPGQ
                     GEGGRFWCRGCNKHGDAVQYLRDFRGLSYLEACHFLGREPKNRHIPIRGRRMKQAHTR
                     RSSPTWEPRECPSPGEAWIQKAGRFVAWAEGKLWTDQGKETRAWLHGRGFSDETIRKF
                     HLGWNPRDFRRDRETWGLPKEPGRGGKPKALWLP"
     gene            complement(801..2438)
                     /locus_tag="PLM79_18565"
     CDS             complement(801..2438)
                     /locus_tag="PLM79_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HOV88366.1"
                     /translation="MTERESPAFAGPGDKQDVSQNVNYFVTDPTQEVKHSSPTKAGEI
                     REKILAVVEESRAKFAHTTTTESQAWTAAQDFFPSRPFPWETFPSELADGLRQLARAC
                     ATGDSALPGVAFCILGAALGRALSVSPKAGWREPLIFYVGDIRPSGAGKTPAATELVR
                     VLHGRQAKEHLRWKAEADHWRGLPKKERESTPEPRRARSFFVSDLTLEGLRVELDDHP
                     TGGLVVVHEELSSFISCQNAYRGGRGSDREAWISLWSGHPARVVRKAESVFLNGGRVS
                     VFGGIQPGVFRAVFTGQDGLFLDDGTLFRFLLTASDCMFNELTPEAWADEHRRAWEAL
                     VDRAFFWAGERVDSPLTQVLSTEAQGRFIDWRNDLESRIPDFPESFRGFLPKAYSYSL
                     RLTGALHAVHQFSERGEPKTVLTLTDIERGIRAVEFYLGQTVQALQVLENPFHAVQAT
                     DERVIQLAVVLDSLRGHTDSGRLAVGFIHDALNQCLPEHQRFQTPRATGAFLRSLGLT
                     IPRRKHDANGRRAVSCLLWDGKIETFLERRLQSLHSL"
     gene            complement(2449..2646)
                     /locus_tag="PLM79_18570"
     CDS             complement(2449..2646)
                     /locus_tag="PLM79_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88367.1"
                     /translation="MERIKKRLFPLAEWDKNPPIAGLRRATLYKSRTLKRWPDLFVKF
                     CGRVFVDLDVLEKYIADGRGQ"
BASE COUNT          581 a          872 c          814 g          591 t
ORIGIN      
        1 aggggagcca tagggccttg ggtttcccgc ccctgccggg ttccttgggg agcccccagg
       61 tttcccggtc ccgccggaag tccctcggat tccaacccag gtgaaacttg cggatggtct
      121 catcggagaa gccccgccca tgaagccatg cccgcgtttc cttgccctgg tcggtccaaa
      181 gttttccttc ggcccaggcc acgaagcgcc cggccttctg gatccaggct tcccccggcg
      241 aagggcattc cctcggctcc caggtggggg agctgcgcct tgtgtgagct tgcttcatcc
      301 tacgcccccg gattggtata tggcggttct tcggctcccg gcccaggaag tgacaggctt
      361 cgaggtagga gagcccccgg aagtcccgaa ggtactgaac ggcgtctccg tgcttgttgc
      421 atccccggca ccaaaagcgc ccgccttcgc cttgccccgg ccaaacccgg aagcggtcgt
      481 ctcccccaca ggaaggacac ggcccggcat attcgcctcc gttggtgcct gaaacccgcc
      541 gggtgggggt tccgtcctgg ttgagaagct cgataagggt cacggtcttt acctctgtct
      601 gtcgggttga aaaaatattt tctatgcaaa ctcgtctgca atgtctgcaa tgtctacaag
      661 cccttgtcct gaaaggcttt ctggatgcag acattgtttt ctcgacgtct gcaatgtctg
      721 caagtttcca tcctgaatgc agacgttgca gacatagcct tttcaatgtc tgcgtccgta
      781 gtggcttgcc tggaaaggct ttacagacta tgcagacttt gcagacgtct ttctaagaaa
      841 gtttcaattt tgccgtccca caacaagcat gagacggccc gtctgccgtt ggcatcgtgt
      901 ttccttcggg gaatggtaag tcccagggac cggagaaagg ccccagttgc cctcggagtc
      961 tggaagcgtt ggtgctccgg gagacattga ttcaacgcat catggataaa ccccacggcc
     1021 agccgccccg aatcggtgtg tcctcgaaga gagtcaagca ctacggccaa ctgaatcact
     1081 ctctcatcgg tcgcctgtac tgcgtggaac ggattctcga gcacttgcag cgcttgaacc
     1141 gtctgcccca agtaaaactc cactgcacga attccccgtt cgatgtccgt cagggtcaaa
     1201 accgtcttcg gctcgccccg ctcagaaaat tggtgcaccg catgcagcgc cccggtgagc
     1261 cgcaacgaat acgagtaggc tttgggtaag aacccccgga agctttccgg gaagtcaggg
     1321 attcgggatt cgaggtcgtt gcgccagtcg atgaagcgcc cctgggcttc cgtgctcaaa
     1381 acctgggtga ggggggagtc aacgcgctca cccgcccaaa agaaagcccg gtcaacaagg
     1441 gcttcccagg cccgccggtg ctcgtcggcc caggcctcgg gggtcagctc gttgaacatg
     1501 caatcagagg ctgtgagcag gaatcggaag agcgtgccgt cgtcaaggaa gagtccatcc
     1561 tgcccggtaa atacggcccg gaaaacccca ggctgaatcc cgccgaaaac ggaaacccgc
     1621 cccccgttaa ggaacactga ctcggccttc cgcaccacac gcgcagggtg tcccgaccaa
     1681 agtgaaatcc atgcctcccg atccgaacca cgccccccgc gataggcatt ttggcaagag
     1741 atgaacgaac tcaactcctc gtgaacaaca acaaggcccc ctgtagggtg atcgtccaac
     1801 tcgacccgga gaccttccag ggtcaaatcg gagacgaaga acgaacgcgc ccgcctgggt
     1861 tcaggggttg attcccgttc cttcttgggt aggccccgcc aatgatccgc ctcggccttc
     1921 catctcaagt gctccttcgc ctgtcggcca tgaagcacgc gaaccaattc ggtcgcagcg
     1981 ggtgttttcc cggcccccga aggccgaatg tcccccacgt aaaaaatgag aggctcccgc
     2041 catccggctt tgggggagac ggaaagggcg cgccccagtg ccgcccccag gatgcagaag
     2101 gcaacgcccg gcaaggcaga gtccccggtc gcgcaagccc gcgcaagctg acgcaggccg
     2161 tccgccagct cggaggggaa tgtctcccat gggaagggtc gcgaggggaa gaagtcctga
     2221 gcagctgtcc aggcttggga ttcggtggtg gtggtgtgag cgaactttgc ccgcgattcc
     2281 tccaccacgg ccaggatttt ctcccggatc tctccggcct tggtggggga agaatgcttg
     2341 acttcctggg tgggatctgt cacaaaatag ttaacatttt gagacacatc ttgtttgtcg
     2401 cccggccccg cgaaggccgg gctttcccgt tcggtcatgg tctccccctt attgcccgcg
     2461 cccgtcggcg atgtatttct ctaggacgtc caggtcgacg aataccctcc cgcagaattt
     2521 gacgaaaagg tccggccaac gtttgagagt gcgggacttg tagagggtgg cccgccgaag
     2581 tccggcaatc ggaggattct tgtcccactc tgccaggggg aaaagccgct ttttgatccg
     2641 ttccatgaat gatccctttc atcgaaaagt ttttgctaca cggtcacaac cggctcaagt
     2701 tgcccaaaat tgaagcacat ccagccggtt acggaaaata ggcgtctacg gatccgctga
     2761 ttccgatagc cgcagaacgg ctctgagctt ggttttaaaa aggacgatcc gggaatgtga
     2821 gtctcatttg gaaggcgttt actgctattc tgaaatgc
//
LOCUS       DAOOVC010000291         2825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_19212_length_2825_cov_8.252708, whole genome shotgun sequence.
ACCESSION   DAOOVC010000291 DAOOVC010000000
VERSION     DAOOVC010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19212_length_2825_cov_8.252708"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..958)
                     /locus_tag="PLM79_18575"
     CDS             complement(131..958)
                     /locus_tag="PLM79_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOV88368.1"
                     /translation="MHFEGTDRYYLNPELREIVNIAIAMEKPLLLTGEAGTGKTQLAF
                     EIARALDYGMEEARCKSTFKGEELCYVYDTVLRLNDSRFGSGDSGRDVNDIWSYLRFG
                     PIGRAFLAEDRRVLLLDEIDKTDSDTQDNLLDVLEDSSFVIREINHKVQARFKPVILI
                     TSNAKRELSDPFLRRCFCHHIPFPSPDEMVRIVRLHFPEIGEPFLEACLTSFYRLREQ
                     GYEKPPATAELLDWIGAMQRAGVRAPGAGREIAYPGTLLKRTQDLLKYKGTWKPGRF"
     gene            1172..1248
                     /locus_tag="PLM79_18580"
     tRNA            1172..1248
                     /locus_tag="PLM79_18580"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1206..1208,aa:Arg,seq:tcg)
     gene            1268..1343
                     /locus_tag="PLM79_18585"
     tRNA            1268..1343
                     /locus_tag="PLM79_18585"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Lys,seq:ctt)
     gene            2303..>2825
                     /locus_tag="PLM79_18590"
     CDS             2303..>2825
                     /locus_tag="PLM79_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOV88369.1"
                     /translation="MLEAMVRSCAGLDVHRRQVVCTVIREGKHETREYGAFYRELKEL
                     ARWLQLEQVEIAVMESTGIFWKSVYASLEEAGIEGRVVNARHVKQVPGRKTDVQDSEW
                     LAELARCGLLRPSFIPPRDLRELRLLTRYRRKLTGVLAGEKNRLHKVLDDAGIRLGCV
                     VSDIDGVSARAMVE"
BASE COUNT          579 a          758 c          919 g          569 t
ORIGIN      
        1 cacgtagcgg agactcacgg gaatgcccgc ccggcgaagc tggtaaaaga aatcgatcat
       61 ggcggaaacg ctcttcctcc gggagacttg ggcggaaagg cgcggggcgg cgccgaaaag
      121 tctccggacc tcaaaaacga cccggcttcc aggttccctt gtacttgaga agatcctggg
      181 tgcgcttgag aagggtgccg gggtaggcga tctccctgcc ggcgccggga gcccggaccc
      241 ccgcccgttg catggccccg atccagtcca ggagctcagc ggtggcaggg ggtttttcat
      301 atccctgttc cctcaggcgg tagaagctcg tcaggcaggc ctcgagaaag ggctcaccga
      361 tctcggggaa gtggagccgg acgatgcgca ccatttcatc gggggaaggg aaggggatgt
      421 gatgacagaa acagcggcgc aaaaacgggt ccgacaactc cctcttggcg ttgctggtaa
      481 tgaggatgac gggcttgaaa cgggcctgca ccttgtggtt gatctcccgg atgacgaacg
      541 agctgtcttc gaggacatcc aggaggttgt cctgggtgtc ggaatcggtc ttgtcgatct
      601 cgtccaggag gagcacccgg cgatcttcgg caaggaaagc gcggccgatg ggcccgaaac
      661 gcaggtagct ccagatgtcg tttacatccc ggcccgagtc tcccgagcca aaccgcgaat
      721 cgttcaagcg caggacggtg tcgtatacgt agcagagctc ctcgcccttg aatgtggact
      781 tgcatcgagc ctcctccatg ccgtagtcca gggcccgggc gatctcgaag gcgagctggg
      841 tctttcccgt cccggcttcc cccgtcagca gaaggggctt ttccatggcg atggcaatgt
      901 tgacgatttc ccgcagctcc gggttcaggt aatagcggtc cgttccttca aaatgcatgg
      961 agggagactc cgtgtagggg ttccggccgg tcggttttcc cggctcagat tgggtccggg
     1021 gccgaggttc ttatcatagt caaaccccgg gacgttgaca atgcgaacgg gtgaccgccg
     1081 ggagaaaagg ggaaaagaga acccgagaaa aagctcttga cacgttggcg ggttttattt
     1141 tagataacaa ctcctttggg acgtgaaggt tgcgcccgta gctcagcagg atagagcgtc
     1201 ggacttcgaa tccgcaggtc ccccgttcga gtcggggcgg gcgtaccagc atactcccga
     1261 ggcaatcggg ccgttagctc aattggtaga gcagctgact cttaatcagg aggttcgggg
     1321 ttcgatcccc tgacggccca ccagtaaaat caaggggtta cggagaatcc gccgtagccc
     1381 ctttttgctc gatacctatt ttgtccccac atagtcccca ctttgggaaa gatgagggcc
     1441 aagtctgtgt cctgtaacgg cgcgcccggt gaagagcaca aacacccata gaccggcgtc
     1501 ttccatcgtc acaacaaaac acgtctcagg gtctcggaag tctcgaaacc caggcgggat
     1561 cgccggttgc cccaggggtt cgtcccggcg gaatgtgtca agcgttttga gacacccggt
     1621 tcagaaaatt agtgaacagc gtgacgctcg cccgtacgct ttgatggcgt ctgtgggcaa
     1681 gtggtcttta agaccgtcat gaagaaacgt cgtgtgccgc cgccgtccga accgcctccg
     1741 acgagtcttg ggggacccat cctcggcggt ccggacggct cactccgagc cgtcaggtcg
     1801 cgggaggctc atgcagcccg gcaatcgcct cacagtgccg acgaagttgc tgcctgcaag
     1861 gcaaggcttt cctcgattct ccccggaggg ggtgggttga tccaaacccc cttcggcaaa
     1921 gggttgattt ggggaggccc gttgacgaag cgctcggggt ataattcccc ccagattttg
     1981 gacagcatgt taagcttaac ggtcatgcat cgggggccgg cgatgcaccc ccagtgatga
     2041 aaatatgctc gagtctcggt agactgttgc gcgtgcggca acgggaaagg accgaggata
     2101 gtccttggtg gttactttga ccccgtgact taacgtggtc cgggcttttc ggcacgcacc
     2161 cccgagtgtt ccgtctccct gagacaggat gtacggtaaa aagacctgcc tgtccgagag
     2221 gacagtgcaa ggcctcccgg gaagccttcc cgatgtcgcc aatgaactga tccaaaggag
     2281 accggggaag gaggtgagag agatgttgga ggccatggtc cgtagttgtg cgggtttgga
     2341 cgtgcatcgt cgacaggtgg tttgcacggt gattcgagag ggcaagcatg aaactcggga
     2401 atacggggcg ttttatcgag aattgaaaga actggcccgt tggcttcagc tggagcaggt
     2461 cgagatcgca gtgatggaaa gcacggggat cttctggaaa tcggtctatg cctctttgga
     2521 ggaggccggc attgaagggc gggtagtgaa tgcccgacat gtgaaacagg ttccggggcg
     2581 aaagaccgat gtccaggaca gtgagtggtt ggctgaactg gcccggtgcg gcctgttgcg
     2641 ccccagtttc atccccccgc gagacttgcg ggaattgcga ctgttgaccc gttaccggcg
     2701 taagctcacc ggcgtgcttg cgggagagaa gaatcggctc cacaaggtcc ttgatgatgc
     2761 gggaatccgg ctgggttgtg tggtcagcga cattgatggc gtatcggctc gggcgatggt
     2821 ggagg
//
LOCUS       DAOOVC010000292         2790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_19541_length_2790_cov_6.880804, whole genome shotgun sequence.
ACCESSION   DAOOVC010000292 DAOOVC010000000
VERSION     DAOOVC010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19541_length_2790_cov_6.880804"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLM79_18595"
     CDS             <1..711
                     /locus_tag="PLM79_18595"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOV88370.1"
                     /translation="SIAISVIPPFTPVNPSPFPVVAEIVGSHDYGSEELKAFELGYRF
                     QANKSLAVDISGFYNLYDDLRTTERGRRRFELFPAPHFVIPLKAANRMDGETYGVEVA
                     ADWHPLKWWRLNAAYTFLYMDLETDRDSTDLYTAKSTEGVSPRHQFSFRSRTELPKNL
                     ELDLWLRYVDSLSGIDVPGYVTMDARLAWRPQKGLELAVVGQNLLDAGHLEFRPDFLN
                     SLPTEIQRGVYGKITWSF"
     gene            775..1332
                     /locus_tag="PLM79_18600"
     CDS             775..1332
                     /locus_tag="PLM79_18600"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOV88371.1"
                     /translation="MIASYRVCTIAWIVVAIVAGSILPRELNGADAEEYRVKLAFIYN
                     FIKFTQWPENAREDGRSSIVLGVLGEDPFGPALESLDGKVVGDRKLQTRRLKNLEEAA
                     ACRVLFVSLSEQGRLEPIMRVLRSRSILTVSDIPGFIQYGGVIGLVFVENKVRFQVNV
                     FAARLSGLQISSKLLRLAEHVKEND"
     gene            1352..>2790
                     /locus_tag="PLM79_18605"
     CDS             1352..>2790
                     /locus_tag="PLM79_18605"
                     /inference="COORDINATES: protein motif:HMM:NF028461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE sensor domain-containing protein"
                     /protein_id="HOV88372.1"
                     /translation="MRIFRDIPIKRKLTAIIMLITGITLVLATMGWVAGELIAFRLNI
                     QQNLRTMAEVIGINCAAPLAFGDRKSAQEVLGALSAVPNVVLAQVYTADNELFAEYRN
                     PKSDSNVGGSSISPSEGTRSLRLESFFTNHLDLSSPIRLDHEIIGRVTLRSDLGQFYD
                     RVRDYGISVVLIVGISAFLAYFLSSIFQRVITAPITALGETMKSVSAKKDYTIRMEED
                     RRDEIGALIAGFNDMLSQVHQRDEILRMHQERLEEEVASRTAELSASNRNLEWTVAEL
                     NLAKDAAESASRAKSQFLANMSHELRTPMNGVLGMTELLLGTELNPQQRNLAETALRS
                     GEALLSVLNDILDFSKIEAGKMELEDIDFDLREPMEAVVELLAEHARKRGIELLCRIG
                     PEIPVALRGDPGRLRQVLTNLVGNAVKFTEQGEVVVSVRLSEQDEGSVVLEFNVRDTG
                     VGIPGERLAHIFDAFSQADGSMTRRFGGTG"
BASE COUNT          588 a          843 c          812 g          547 t
ORIGIN      
        1 agcatcgcca tatccgtcat cccgccgttc acgcccgtca atccttcccc gtttccggtg
       61 gtggcggaga tcgtgggttc ccacgattac ggttccgagg aattgaaagc cttcgagctg
      121 ggctaccgct tccaggcaaa caagagtctc gccgtggata tatccggctt ctacaatctc
      181 tacgacgacc ttcgcaccac cgaaaggggc agacgccgat tcgaactgtt tcccgcaccc
      241 cattttgtca tccccctgaa ggccgccaac cggatggatg gagagaccta cggagtggaa
      301 gtggcggccg attggcaccc gctgaaatgg tggcggctca acgcggccta caccttcctg
      361 tacatggacc tggaaacgga ccgggacagc accgacttgt acacggcaaa gtccaccgag
      421 ggcgtcagcc ctcgtcacca gttttccttc cgttcgagaa cggaactccc caagaatctc
      481 gaattggacc tgtggcttcg ttacgtggat tcgctctccg gcattgacgt tcccggctac
      541 gttaccatgg atgcccggct ggcctggcgg ccgcaaaagg gcctcgagtt ggccgtggtg
      601 ggccagaatc tcttggacgc cggccatctt gaattcagac cggatttcct caattctctg
      661 cccacggaaa tccagcgagg ggtttacggc aagattacct ggagcttcta gttccggatc
      721 ggcacaacac ggaaggtcgt tttcgagctc ttgttcgacg gcgttgggag aatcatgatc
      781 gcgtcctacc gggtatgcac cattgcatgg attgttgtgg cgatcgtcgc gggctcgatt
      841 ctccctcgag agctgaatgg ggccgatgcg gaggaatacc gcgtcaaact agcgttcatc
      901 tacaatttca tcaagttcac ccagtggccg gagaatgccc gggaagacgg aaggtcttcc
      961 attgtgctgg gggtcctggg agaggatccc ttcggcccgg cattggaaag cctggacggg
     1021 aaagtggtgg gggacaggaa actgcaaacc cggcgtttga agaacctcga ggaggcggcc
     1081 gcatgccggg tccttttcgt cagcctttcg gaacagggtc gcctggaacc cattatgcgc
     1141 gtccttcgct cacggagcat cctcaccgtc agcgacatcc ccggattcat tcagtatgga
     1201 ggcgtcatcg gtcttgtgtt cgttgaaaac aaggtccgct tccaggtcaa cgtctttgcc
     1261 gcccgtctat cgggcctgca aatcagctcc aaactgctca ggctggcgga gcacgtcaaa
     1321 gaaaacgact aggcccccgg ggtaggaaaa gatgcgaatc ttccgagaca ttcccattaa
     1381 gagaaaactg accgccatca tcatgctgat caccggcatc accctggttc tcgcaaccat
     1441 ggggtgggtc gccggggagc tcattgcctt ccgcctgaac atccagcaga acctcaggac
     1501 catggccgag gtcatcggca tcaactgcgc cgctcctttg gcctttggag atcgaaagag
     1561 cgcccaggaa gtcctgggag ctttgagtgc cgtgccgaac gtagtccttg cacaggtgta
     1621 tacggccgac aacgaattgt tcgccgaata tcggaacccc aaatccgatt ccaatgtcgg
     1681 cggaagctcg atctctccct ccgaagggac ccggtccctc cgattggaat cgttcttcac
     1741 gaatcacctg gacctcagca gcccgatccg cctcgaccac gaaatcatcg gcagggtgac
     1801 cctgcgctcc gacctcgggc aattctacga ccgagtgagg gactacggca tcagtgtggt
     1861 gttgatcgtg gggatctcgg ccttcctcgc ctatttcctc tcctccattt tccagcgcgt
     1921 gatcacggcc cccatcaccg ccctgggaga aaccatgaag tccgtctccg ccaaaaagga
     1981 ctatacgatc cgaatggaag aggaccgacg ggacgaaatc ggcgcgctca tcgcaggctt
     2041 caacgatatg ctctcccagg tccaccagcg ggacgaaatc ctcaggatgc accaggaaag
     2101 actcgaggaa gaggtggcta gccggaccgc cgagctttcc gcgtccaacc ggaacctgga
     2161 atggacggtg gcggagctga acctggccaa ggatgcggcg gaaagcgcaa gccgggccaa
     2221 gtcccagttc ctggccaaca tgagccatga gctccgcacg cccatgaacg gggtcctggg
     2281 aatgaccgaa ctcctgctgg gaaccgagct caacccgcag caacgcaacc ttgccgaaac
     2341 ggccctgcgc tcgggagagg ccctgctgag cgtactcaac gacatcctgg atttttccaa
     2401 gatcgaagca ggcaagatgg aactggaaga catcgatttc gatcttcggg aacccatgga
     2461 ggcggtggtg gagctcctgg cggagcatgc ccgcaagagg ggcatcgagc tcctgtgccg
     2521 gatcggaccc gaaattcccg tcgctctgcg gggggacccg ggacgcctgc gacaggtgct
     2581 cacgaacctc gtgggaaacg ccgtcaagtt taccgaacag ggcgaggtcg tggtttccgt
     2641 gcgcctgtcg gaacaagacg agggctccgt tgttctcgaa ttcaacgtcc gcgatacggg
     2701 ggtgggaatt cccggcgaaa ggcttgccca cattttcgac gccttctccc aggccgacgg
     2761 ttccatgacc cgcaggttcg gcggcacggg
//
LOCUS       DAOOVC010000293         2739 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_20031_length_2739_cov_3.169523, whole genome shotgun sequence.
ACCESSION   DAOOVC010000293 DAOOVC010000000
VERSION     DAOOVC010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20031_length_2739_cov_3.169523"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLM79_18610"
     CDS             complement(<1..1241)
                     /locus_tag="PLM79_18610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOV88373.1"
                     /translation="MRSDTNTSPLDLLRTYFGYDAFRGHQEEIIHHVLSGGDALVLMP
                     TGSGKSICYQIPAMMRSGVGIVISPLIALMQDQVDTVRQLGIRAEFLNSTLNPREARA
                     VELKMVSGEIDLLYVAPERLLTPEFQQLLNQTPLALFAIDEAHCVSQWGHDFRPEYLQ
                     LSVLPDRFPHIPRIALTATADPVTRREIIEKLKLENARQFISSFDRPNIYYRVELKHN
                     ERAQLLDFIQTEHPEDSGIVYCLSRKRVDSTAKWLSERGLPVLPYHAGMDQNLRLRHQ
                     RKFLQEERVIMVATIAFGMGIDKPDVRFVAHLDLPKTLEGYYQETGRAGRDGERADAW
                     MVYSLADVVALRQMLEASDGGEQFKRIQQQKMQAMLGFCETARCRRQVLLGYFGEELP
                     KPCGFCDTCDGKVETWDGTIVA"
     gene            complement(1266..1901)
                     /locus_tag="PLM79_18615"
     CDS             complement(1266..1901)
                     /locus_tag="PLM79_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88374.1"
                     /translation="MTIFSTAGFREGRWAGGLVELLRRRSIIPGSKPKARIWRATMEQ
                     WIQFETGLIPGPLFWIGKPAIRASTDALYAGYYVERGLPPGNIRGDSGVITPQWHWHG
                     FWRCLTEEDFRTQLHELMLGLQEERRSIWIDAGERSAFLSYTGENSLEEAAGLCSSVP
                     PDYWIDFFIGIRLEKEQCLALQGAIVPEIQNALVRVEEISSLVQCARDGLR"
     gene            complement(1932..2543)
                     /locus_tag="PLM79_18620"
     CDS             complement(1932..2543)
                     /locus_tag="PLM79_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88375.1"
                     /translation="MNRKYLIGYMLLNCLIFLILSGIFSGGLDEIESLYKKTTNIFGF
                     LLIPLSIVFEGLLSPDFKYKLVLWRIENPLPACRAFSEIGPNDPRIDMSRLNLLLCGN
                     IPESPEKENATWYGLYKKCSSKDRVFDAHKCFLLMRDLASLSFTSIPVAVASYTLWGI
                     PLRSMAIHLVFLVAMFLLTSVACQNYGKRFVANVLVESLHTVE"
BASE COUNT          637 a          761 c          703 g          638 t
ORIGIN      
        1 gccacgatgg ttccatccca ggtttccacc ttgccatcgc aagtgtcgca aaagccgcag
       61 ggcttgggca gttcctcacc aaaatagcca aggagcacct gccttcggca cctggccgtc
      121 tcgcagaagc cgagcatggc ctgcatcttc tgttgctgga ttctcttgaa ctgctcaccg
      181 ccatcggagg cctcgagcat ctgccttagg gcaactacat ccgcaaggct gtagaccatc
      241 catgcatcgg ccctttctcc gtcacggccg gcacgaccgg tctcctggta gtagccctcc
      301 agggtcttgg gcagatccag gtgagccaca aagcgcacat caggcttgtc tatgcccatg
      361 ccgaaggcga tggtggcaac catgattacg cgctcttcct gcaggaactt gcgctgatgc
      421 ctcaggcgca ggttctggtc cattcccgca tggtagggga gaacaggcag ccccctctcc
      481 gagagccact ttgccgttga atcgactctt tttctgctga ggcagtacac gatgccggaa
      541 tcctccgggt gttctgtttg aatgaaatca aggagctggg ctctttcgtt gtgcttaagt
      601 tcaacccggt agtagatatt cggccgatca aagctcgaga tgaattgtct ggcattctcc
      661 agcttgagct tttcgatgat ctccctgcgc gtcacagggt ctgccgtggc ggttagagca
      721 atgcggggaa tgtgcgggaa gcggtccggg agaacggaaa gctgaagata ctccgggcgg
      781 aaatcgtgtc cccactggga gacgcagtgc gcttcatcta tggcaaagag cgccagtggc
      841 gtttggttca gaagctgctg aaactccggg gtcaggagac gctcgggagc tacatacagg
      901 agatcgatct caccggagac catcttgagc tcgacagccc gagcttccct cggattcagg
      961 gttgagttga ggaactcggc ccggatgccc agctggcgga cggtgtccac ctggtcttgc
     1021 atcagtgcga tcaggggaga tatgacaatt cccacaccac tgcgcatcat cgccggtatc
     1081 tggtaacaga tggatttccc gctgcccgtc ggcatcaaga caagagcgtc cccgcctgac
     1141 aggacatgat ggatgatctc ttcctggtgg ccgcggaatg catcgtatcc aaagtaggtg
     1201 cggagtaaat ccagtggaga agtattagta tcggatcgca tgattggtat gttacttgcc
     1261 ggagctcatc tcaggccgtc tcttgcacac tgcacgagcg atgagatctc ctcgactcta
     1321 accaaagcgt tttgaatttc aggcacaatc gccccctgta gcgccaggca ctgttccttt
     1381 tccagccgga tgccgatgaa gaaatcgatc caataatcag ggggcacaga actacacagc
     1441 ccggctgctt cctcgagaga attctctccg gtgtacgaga gaaaagcact tctctccccg
     1501 gcatcgatcc atatggaccg acgctcctcc tgtagaccga gcattagttc atgcaattgg
     1561 gttcgaaagt cctcctcagt caaacaccgc cagaacccat gccaatgcca ctgtggagtt
     1621 attacgccac tgtcgccgcg tatgttgccc ggaggcagac ctcgctccac atagtagcct
     1681 gcataaaggg catccgtact ggccctgatt gctggtttgc cgatccagaa gagtggccct
     1741 ggaatcaggc cggtttcaaa ttggatccac tgttccatcg tcgcccgcca tatcctagct
     1801 tttggcttgc ttccaggaat aatggatcgc cttcgaagca gctccaccag cccacccgcc
     1861 caacgcccct ctctaaatcc tgccgttgaa aatatggtca ttcatcttat cctttgggct
     1921 atgaaccaag tttactctac cgtatggagg gattccacaa gaacatttgc gacgaaacgc
     1981 ttgccgtaat tctggcaagc gactgaagtg agcaaaaaca tcgctaccag aaaaaccaaa
     2041 tgaatcgcca tactcctcaa aggtataccc cacaacgtat acgaggcaac ggcaacagga
     2101 atggatgtaa agctcagaga cgccagatct cgcatgagca gaaagcattt gtgggcatcg
     2161 aagacacggt ctttggatga gcacttcttg tagaggccgt accacgtcgc gttttctttt
     2221 tcaggagatt cagggatatt gccgcacaaa agcaggttaa ggcggctcat gtcgattcga
     2281 gggtcattgg gaccgatttc actgaatgcc ctgcatgctg ggagagggtt ttcaatcctc
     2341 cataagacga gcttgtattt aaaatccggg ctcaagagtc cttcgaaaac tatggacagg
     2401 gggatgagca gaaatccgaa tatgttcgtt gtcttcttat aaaggctctc tatctcgtcc
     2461 aatccaccgg agaagatacc cgacagtatc aaaaagatca ggcagttcaa aagcatgtag
     2521 cctatgaggt actttctgtt catgctctta atgtcttttt cttgtcccat aaagacccct
     2581 ttcatcaaca cgggctatcg agttgaacga caaccctgtt cttatgcgtc ctcgatcctc
     2641 ttcaagcaat cctgccatcg tcgatgagga tttcataccg ctccggtgtc cagataaggc
     2701 tgatgcacgg ggctccaagg cgatgagccc tcatcttca
//
LOCUS       DAOOVC010000294         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_21616_length_2592_cov_4.039811, whole genome shotgun sequence.
ACCESSION   DAOOVC010000294 DAOOVC010000000
VERSION     DAOOVC010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21616_length_2592_cov_4.039811"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..825)
                     /locus_tag="PLM79_18625"
     CDS             complement(223..825)
                     /locus_tag="PLM79_18625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOV88376.1"
                     /translation="MNDECGMMSAESAMIISSFIIHHSSFIIHHSKRWDSMSNFKQKN
                     HLPYTFRLISWRKQSVNLDIGGGQFNLATDYLAKHGVRNLVYDPFRRGAEHNDLILKL
                     VDENPVDTVTVNNVLNTIREESERLKVIRLAAKALKPGGTAYFLIHEGDRSGLGCLTL
                     FCWQNNMRTDAYLSEISRYFKSVRISRNLIIAGIPHDYQE"
     gene            complement(822..1631)
                     /locus_tag="PLM79_18630"
     CDS             complement(822..1631)
                     /locus_tag="PLM79_18630"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HOV88377.1"
                     /translation="MRNPGQLPAPTGQFANSVRPAGEDEPGDVLDLMVNEALKFIKAH
                     EPPEGYFIAFSGGKDSIVSLELTRMAGVRHQAFYSCSSIEPPEVVQFIREFYPEVTFL
                     YPERSFWELIRKKFPPLKTKRWCCDFLRKYPSRKIPLKHRIMGMRMEESRDRADRPRI
                     ERNKREKTIVFKPLFSWKAWSIWDFIQRYRLPYPSLYDEGWERVGCVVCPFICSPTMK
                     HIERNRERWPGIYRAFEHAVTDWFLERKARGANLREQTPEEYITNWYKGQG"
     gene            complement(2076..2381)
                     /locus_tag="PLM79_18635"
     CDS             complement(2076..2381)
                     /locus_tag="PLM79_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88378.1"
                     /translation="MDCRHFISCSANLCPLDSCLSLRSWFIGEDICKLKPHSDHPLVK
                     RQKQLNRRRPKEHLEKPLFADWLTISTPKKRNLTRERREALSQKMKNINKAKIPKST"
     gene            complement(2409..>2592)
                     /locus_tag="PLM79_18640"
     CDS             complement(2409..>2592)
                     /locus_tag="PLM79_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88379.1"
                     /translation="STAYKDHILTLAVQSKWFDKATHAFFLNDGRVQSIISRFLKDDT
                     THPLWWSDIGGTVRRV"
BASE COUNT          543 a          648 c          694 g          707 t
ORIGIN      
        1 cacctcgccc cttgacatgc gcaccggccc atgcgacact ggcccccacg acaaagagaa
       61 agtctttttg agagtcgttc atgcctggaa aaccaaggga agagacctga taaatccatt
      121 gtggaattca ccatgcggca gacagtaatc caatttgtgt tgctcattgg cctgagcagc
      181 tgaatagctc gatgatgctg aagattgggc ggcctattcc gcttattcct gataatcgtg
      241 tgggatgcct gcaatgatca ggttgcggga gatccgaacc gacttgaagt acctgctgat
      301 ctcagagagg taagcatccg tcctcatgtt attctgccag caaaagagag tgagacatcc
      361 caatccactt ctgtcacctt catggataag gaagtaggct gtccctccgg gtttcaacgc
      421 ttttgcggcc agccttatga ccttcaatcg ctcgctttct tcccgaattg tgttgaggac
      481 attgtttacc gtcaccgtgt ctactggatt ctcgtccacc agcttcagta tgagatcgtt
      541 gtgttctgca ccgcggcgga atggatcata aaccaggttc ctgactccat gttttgccag
      601 gtaatccgta gccagattga attgccctcc gccgatatcg agattgacgg attgcttccg
      661 ccatgaaatc aatcggaaag tatacgggag gtggttcttt tgcttgaaat tgctcatgct
      721 atcccacctt tttgaatgat gaatgatgaa tgatgagtga tgaatgatga atgatgaaat
      781 gatcatagct gattctgcac tcatcattcc gcattcatca ttcatccttg tcctttgtac
      841 cagttggtga tgtactcctc aggggtttgc tctcgcaggt tggctcccct ggctttcctt
      901 tcaaggaacc agtcggtcac ggcgtgctcg aatgctctgt aaatacccgg ccagcgctct
      961 cggttccgtt ctatgtgctt catggtcggg ctgcagatga agggacaaac gacgcagcca
     1021 accctctccc agccttcatc gtagaggctc gggtatggaa gccggtatct ttggatgaaa
     1081 tcccaaatgc tccaggcctt ccaggaaaat aggggcttga aaacaatagt cttctccctt
     1141 ttgttgcgtt ctattcgagg cctgtcagct cgatctcttg actcctccat gcgcattccc
     1201 atgatcctgt gctttagggg aatcttcctc gaggggtatt tacgcaaaaa gtcgcaacac
     1261 caccttttgg tcttgagggg cgggaatttt ttccttatga gctcccaaaa gctcctttca
     1321 ggatagagga aggtgacctc gggatagaac tcccttataa actgcaccac ttcgggcggt
     1381 tcgatgcttg agcacgagta gaacgcctgg tggcgtactc cggccatccg ggtcagttcg
     1441 agtgagacga tcgagtcctt gccgccgcta aaggcaatga aatatccctc cggtggctca
     1501 tgggccttga tgaatttcaa agcctcgttg accatgagat ccagcacgtc accgggttcg
     1561 tcttctccag ctggtctcac agaattggca aattggcctg tcggtgcggg cagttgacct
     1621 gggtttctca tgccttcagc ctcttacagc gggccagcct atccattgaa tccaacccac
     1681 agcacggtcc gaagcgccaa ttccaagcgt tgcactcgat cctcatccag acacacatca
     1741 tccgaagctc cttgagcttc tgggcggtgg gtttccattg cattaccgga tgatccattg
     1801 gcagtctggt ctggttcatc ggagcatctc ccaatccgtt tgagtctctg gagagcggta
     1861 aaatccatct atcggggaat gatctatcgg gggaagtgaa tgtatatcta tcaatcatca
     1921 aatacccatc cctcccccta ttatccccct cccttcccgt ggaggttgcg ggatggtgtc
     1981 tgggatggcc gcagaggcga tggggtgcca aagttaccgg ggtttggaag atccgctccc
     2041 cggggtgggt agttgggata gggggagagg taaggttacg ttgacttcgg tatttttgcc
     2101 ttgttgatgt tcttcatttt ttgggagagg gcttcgcggc gctctcgggt caggtttctc
     2161 ttctttggag tggaaatcgt gagccagtcg gcaaagaggg gtttttccag gtgctctttg
     2221 ggtcgccttc tgttgagctg cttttgtctt ttgaccaagg ggtgatctga atggggtttg
     2281 agtttgcaga tgtcttcgcc gataaaccag gagcgtaggg aaaggcagga atcgagcggg
     2341 cagaggttgg cgctgcagga tatgaagtgt ctgcaatcca tcttcttttc ttccgctcct
     2401 agatgatttc acacacgtcg cactgttccg ccgatgtcgg accaccatag cgggtgagtc
     2461 gtgtcgtctt tgaggaagcg gcttatgatg gactggactc ggccgtcgtt taggaagaag
     2521 gcgtgcgtgg ccttatcaaa ccatttgctt tggactgcga gggttaggat gtggtccttg
     2581 taggcggtcg ag
//
LOCUS       DAOOVC010000295         2591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_21629_length_2591_cov_7.977129, whole genome shotgun sequence.
ACCESSION   DAOOVC010000295 DAOOVC010000000
VERSION     DAOOVC010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21629_length_2591_cov_7.977129"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..481
                     /locus_tag="PLM79_18645"
     CDS             95..481
                     /locus_tag="PLM79_18645"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOV88380.1"
                     /translation="MRWLKTYVEKCINCGKCMETCSKTYFKEENPSLSRVVVTNPGAG
                     QNISVCNQCGACMAVCPTEALYRDKNGVVQLDKSKCTSCLMCVGFCPTGNMLYAASKQ
                     TEPFKCVACGLCAKVCPTQALVLVND"
     gene            512..>2591
                     /locus_tag="PLM79_18650"
     CDS             512..>2591
                     /locus_tag="PLM79_18650"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOV88381.1"
                     /translation="MEKMKVIAEWTYTPAPIERGYTRKTLYVNVGEPRMEIKDVSQDM
                     IDTFTGGRGFDLKLLWDAVKDTTKWNDPENEIVLSGGPLCGITQYPGAGKCYAAFISP
                     LSGQTYDSNSGGYWGPLSKFSGFDAVEVQGKAEKDIILFINGDEGKIQILESNLKDLN
                     AYAVTETLHEYFAVDEADKRNISVISTGKAAETSYYGCMNMSFYDPRRKVARLKQAGR
                     GGGGSIMRDKKIAAMVVKFSKIGGNLNNVVDLDTLQRVGVKLHKEIHDLDDVQCKMRK
                     VGTAHLNEIMDEYHLLPTNNYKFGQNAAEISKIHSAVYERLFTQGMPDGCWYGCSMSC
                     AKAVDGFTVKTGPCKGEKVTVDGPEYETVAALGSNIGVYDPLWTIEANFYADHYGMDT
                     ISLGTAIAFVCECYELGLITKKDTNGLELNFGSKDDIMELIHRMADGKDEFASMVAKG
                     IRYMKKAFAEKFKADAKTMEAIGMEGQGVETSEYVPKESIAQWGGYYLTLKGPQHDEA
                     WLIFMDMVNKQLPTFEDKAEALHYFPNFRTWFSLVGLCKLPWNDVEPANNRIVNEPKE
                     AAKVPEHVQNYVDIFNAVTGKGIRKEDLITMSEKVYNFQRVFQVRMKQGEANHNIPLR
                     ALGPVFPDEWEARREYYDGKLREIGIDPSALSTEEKIKKLQEYRLGMWEGLKMAVYKR
                     RGWNKKGIPTV"
BASE COUNT          646 a          692 c          738 g          515 t
ORIGIN      
        1 ttgtgcaatc ttgcgcttgt tcctcccctt ctgtatactt aatcttgccg tcgaaacgag
       61 cagattcgtt cagaccgacc aaggggggat cattttgagg tggctcaaaa cgtacgtgga
      121 aaagtgcatc aactgcggga agtgcatgga aacctgctcc aaaacctact ttaaagaaga
      181 aaacccgtct ctttccaggg tcgtggtgac caatccggga gctgggcaga acatcagtgt
      241 ttgcaaccag tgtggagcct gcatggctgt ctgcccgacg gaagctctgt acagggataa
      301 gaatggtgtc gtacagctgg acaaatccaa atgcacttcc tgcctgatgt gtgtcgggtt
      361 ctgtccgacg gggaacatgc tctacgccgc ttcgaagcag accgagccct tcaagtgcgt
      421 ggcttgcggc ctttgtgcca aagtctgccc cactcaggct ttggtgctcg tcaacgatta
      481 ggataatcaa agaaggaggg ctgggtttct gatggaaaaa atgaaagtta tcgcggaatg
      541 gacctacaca cctgccccaa ttgaaagggg ctatacgcgc aagaccctct acgtcaacgt
      601 cggcgaaccg cgcatggaaa tcaaggatgt gtcccaggac atgatcgaca ccttcaccgg
      661 cgggcgcggt ttcgatctca agctgctctg ggacgcggtc aaggacacca cgaaatggaa
      721 cgaccccgag aacgagatcg tgctgtcggg cgggccgctc tgcggaatca cccagtatcc
      781 cggagccggg aagtgctacg ccgcttttat ctccccgctt tccggccaga cctatgatag
      841 caactccggc ggatattggg gccctctttc caaatttagc ggattcgacg ccgttgaggt
      901 ccagggaaag gctgaaaagg acatcatcct ttttatcaac ggggatgaag gcaaaattca
      961 gatcctcgag tcgaacttga aagatctgaa tgcctacgcc gtcaccgaaa cgcttcacga
     1021 gtatttcgcc gttgacgaag ccgacaagcg gaatatttcc gtcatctcca cggggaaggc
     1081 tgccgagacc tcctactacg gctgcatgaa catgagtttc tacgatccac gccgtaaggt
     1141 ggcccgcctg aaacaggccg gccggggcgg cggcggttcc atcatgcggg acaagaaaat
     1201 tgcggcgatg gttgtcaagt tttccaaaat tggcgggaac ctgaacaacg ttgtcgacct
     1261 tgacaccctt cagagagtcg gggtgaagct gcacaaggaa atccatgacc tggatgacgt
     1321 ccagtgcaag atgcggaagg tgggcaccgc acacctgaac gagatcatgg acgaatacca
     1381 cctccttccg acgaacaact acaagtttgg ccaaaacgcg gcggagattt caaaaatcca
     1441 ctctgcggtt tatgagcgcc tctttaccca ggggatgccc gacggttgct ggtacggctg
     1501 ctccatgtcg tgtgcaaagg ccgtcgacgg tttcacggtc aagactgggc cctgcaaagg
     1561 cgaaaaagtg acggtcgacg gcccggaata cgaaacggtc gccgcactcg gttccaacat
     1621 cggagtctat gacccgctct ggacgatcga ggccaatttc tatgcagacc attacgggat
     1681 ggacaccatc tccctgggaa ccgccatcgc gtttgtctgc gagtgctacg aactcggtct
     1741 gatcaccaag aaggatacga acggccttga actgaacttc ggaagcaaag acgacatcat
     1801 ggaactgatc caccggatgg ccgacgggaa agacgagttc gcatccatgg tggctaaggg
     1861 catccgctac atgaaaaagg ctttcgccga aaagttcaag gctgacgcca agaccatgga
     1921 ggcaatcggc atggagggac agggtgtcga aacttccgaa tatgtcccga aagagtcgat
     1981 tgcacagtgg ggcggctatt acctgaccct gaaggggccc cagcatgacg aggcctggct
     2041 cattttcatg gacatggtca acaaacagct cccgactttc gaggataagg ccgaggcgct
     2101 tcattatttc ccgaatttcc ggacctggtt ctcccttgtc ggcctttgca agctcccctg
     2161 gaacgacgtt gagccggcca acaaccggat cgtgaacgag ccgaaggaag ccgccaaggt
     2221 tccagaacac gttcagaact atgtcgatat cttcaatgcc gtgacgggga aaggcattcg
     2281 gaaagaagac ctcatcacga tgtccgagaa ggtttacaac ttccagcggg tgttccaggt
     2341 tcgcatgaag cagggcgagg cgaaccacaa tatccccctt cgcgccctgg gtcccgtttt
     2401 cccggatgag tgggaagccc gcagggaata ctacgacggc aagctcaggg aaatcggcat
     2461 cgatcccagc gccctgtcga cggaggaaaa gatcaaaaag ctgcaggaat accggctcgg
     2521 gatgtgggaa ggactcaaga tggcggttta caagcggcgg ggctggaaca agaagggaat
     2581 cccgacggtg g
//
LOCUS       DAOOVC010000296         2564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_21933_length_2564_cov_5.990036, whole genome shotgun sequence.
ACCESSION   DAOOVC010000296 DAOOVC010000000
VERSION     DAOOVC010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21933_length_2564_cov_5.990036"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLM79_18655"
     CDS             complement(<1..513)
                     /locus_tag="PLM79_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOV88382.1"
                     /translation="MPLSASIERLEPCPHPGQSPEAETGRSPRERLWGLAWLCGLCLL
                     WMAGCMVGPNYQPPRSEVPSEWKGVEPGPGGVVSRAVRGAGTLVEWWKSFEDPALVSL
                     IERGIDSNPDLRQATARIREARAARGMGSSALWPSVESGASYLRSGPLTSRSRTTPVG
                     GSSVISGGTVV"
     gene            complement(526..>2564)
                     /locus_tag="PLM79_18660"
     CDS             complement(526..>2564)
                     /locus_tag="PLM79_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOV88383.1"
                     /translation="SGSGKTTLMNILGCLDRPTSGEYWLDGRDITAMSADERALVRNE
                     EIGFVFQNFNLLPRTSAMGNVVMPLSYAPTPPPEGEGRQRAEELLERVGLGAWLLHEP
                     SQLSGGQQQRVAIARSLINRPSLLFADEPTGNLDSQTSEEVLKIFQQLNEEEGMTIVL
                     VTHDENVARHAGRIIRIRDGLIESEERTSPVAERPAPAVLEPAPHRDREKRSGGGGFL
                     MAGRRMLPTALNGLRRNILRAALTTLGIMIGVGAVIAMMEIGRGSSVSIQQTIASMGA
                     NNLLILPGTAASGGVSFGSGSVRTLTPEDAEAIRSEVSGLRAVAPAVRARTQIVYGNR
                     NWVPSVMYGTTPEFLEAREWGLGEGEPFTRQDVRNAGKVCLLGQTIVRELFQGESPVG
                     KEIRINNVPFKVVGVLNSKGANMMGMDQDDVLLAPWTTIKYRVTGSTLGSSNQSASTT
                     GTSSGTTSQTNTTSQLYPNSKTNLYPEQSATQAASNPLQARFTDVDQILAATRSSGDI
                     ASAIEDITRLLRDRHRIRPGKPDDFTIRDMTEMTKAMSSTANMMTKLLLAVALISLMV
                     GGVGIMNIMLVSVTERTREIGLRMAVGARRRSILQQFLVESVVLCFLGGAVGILLGRG
                     ISFVVTLVLQWPTELSVDAILAAFAVSVTVGIVFGYYPAWKASRLDPIIALRYE"
BASE COUNT          454 a          804 c          838 g          468 t
ORIGIN      
        1 caccacggtt ccgccggaga tgaccgagga tcctcccacc ggcgtagtcc gggacctcga
       61 agtcaaaggg ccgctgcgaa ggtaggatgc gccgctttcg acggagggcc acaaggccga
      121 ggaacccata ccgcgagccg cccgggcctc gcgaatccgg gccgtcgcct gccgaaggtc
      181 cgggttggaa tcgatgcccc tctcgatcaa ggaaacgaga gcggggtcct cgaagctctt
      241 ccaccattcc accagggtac cggctccccg gaccgcccgg ctgacgactc ctccgggacc
      301 gggctccacc cctttccatt ccgaaggcac ctcggatcgg ggcggctggt aattgggtcc
      361 caccatgcat cccgccatcc aaagaaggca aagcccgcag agccaggcaa ggccccaaag
      421 gcgctccctc ggggaacggc cggtctctgc ttccgggctc tgcccgggat gcgggcacgg
      481 ttcgagtctt tcgatgctcg cagacaaagg catgaactct cctcgttatt cgtagcgcaa
      541 ggcgatgatg ggatccaggc gagaggcttt ccaggccgga tagtagccga aaacgatccc
      601 cacggtgacc gaaacggcaa aagcggccaa aatggcatcc acggaaagct cggtgggcca
      661 ttggagcacc agggtcacca cgaaagagat cccgcggccc aggaggatcc ccacggcccc
      721 gcccagaaag cacagcacca ccgattccac caggaactgc tgcagaatgc tccttcggcg
      781 tgccccgacg gccatgcgca acccaatctc ccgggtgcgc tcggtgacgg acacgagcat
      841 gatgttcatg atgcccacgc cgcccaccat cagggaaatg agggccacgg ccaggagcag
      901 cttggtcatc atgttggccg tggaagacat ggccttggtc atctcggtca tgtcccggat
      961 ggtgaagtcg tccggttttc cggggcgaat gcggtgccgg tcccgcagaa gccgggtaat
     1021 gtcctcgatg gccgaggcaa tgtcccctga ggaacgggtg gcggccagga tttgatccac
     1081 atcggtgaac cgggcctgga gagggttgct ggccgcctgg gtcgcggatt gttccgggta
     1141 gaggttggtc ttggaattgg gatagagctg gctggtggta ttggtctggg acgtggtccc
     1201 cgaggaggtg ccggtggtgg aagcgctctg attggagctc cccagggtgc tgcccgtgac
     1261 ccggtacttg atggtggtcc agggggccag gaggacatcg tcctggtcca ttcccatcat
     1321 gttggccccc ttgctgttca ggactcccac cactttgaag ggaacgttgt tgatgcggat
     1381 ttcctttccc acgggggact ctccctggaa gagctctcga acgatggttt gtcccaggag
     1441 gcagactttc ccggcattgc gcacatcctg gcgggtgaag ggttctccct cccccagacc
     1501 ccactcccgt gcctcgagaa attcgggcgt ggttccgtac attaccgacg ggacccagtt
     1561 gcggttgccg tacacgatct gggtcctggc tcgtacggcg ggagcgaccg cccgcaggcc
     1621 ggagacctcg gatcggatgg cttcggcgtc ttcgggagtg agggtgcgga cactgccgct
     1681 gccgaagcta accccaccgc tggcggccgt tcccggaagg atgagcaggt tgttggcccc
     1741 catgctggca atggtctgct ggatggacac ggacgagccc cggccgatct ccatcatggc
     1801 gatgacggcc cccacgccga tcatgattcc cagggtggtc aaggccgccc gcaggatgtt
     1861 tctgcgaagg ccgttgaggg cggtgggaag catgcggcgc cctgccatga gaaacccgcc
     1921 ccccccgctc cttttctccc gatcccggtg tggggccggt tcgaggactg caggcgccgg
     1981 tctttcggca acgggagagg tcctctcctc cgattcgata aggccgtccc gtatgcggat
     2041 gatccgcccg gcgtgccggg ccacgttctc gtcatgggtc accaggacga tggtcattcc
     2101 ctcttcttcg ttgagctgct gaaagatctt caggacctcc tcgctggttt gcgaatcgag
     2161 gtttcccgtg ggctcgtctg caaagaggag ggacggacga ttgatgaggg aacgggcgat
     2221 ggccacgcgc tgctgctggc cccccgagag ctgggaaggt tcgtgaagaa gccaagcccc
     2281 cagccccacc ctctcgagga gttcttcggc acgttgccgc ccctctccct ccggcggcgg
     2341 cgtgggggcg taggaaaggg gcatgaccac attgcccatg gcgctggtgc ggggcagcag
     2401 gttgaagttc tgaaagacga agccgatttc ctcgtttcgc accagggcgc gctcatccgc
     2461 cgacatggcc gtgatatcgc gcccgtcgag ccaatactct cctgaggtgg ggcggtccag
     2521 gcatcccagg atattcatga gggtggtttt ccccgacccg gagg
//
LOCUS       DAOOVC010000297         2546 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_22165_length_2546_cov_4.539141, whole genome shotgun sequence.
ACCESSION   DAOOVC010000297 DAOOVC010000000
VERSION     DAOOVC010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22165_length_2546_cov_4.539141"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..578)
                     /locus_tag="PLM79_18665"
     CDS             complement(126..578)
                     /locus_tag="PLM79_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88384.1"
                     /translation="MFRKWSLVLGIALLTPWFVVTPPSVEAGLSGPVCFEVTIPDKGS
                     FQVTGRATPVSGDYIVYHGKATIADENPYYLGGTGMPAGKYVILNLTGTQSHKDGWRD
                     TGIMQIQLNTSTSPMKGTFYEVGTDYDLNSKTFGNHFSKGTLKRINCP"
     gene            complement(888..2015)
                     /gene="ald"
                     /locus_tag="PLM79_18670"
     CDS             complement(888..2015)
                     /gene="ald"
                     /locus_tag="PLM79_18670"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOV88385.1"
                     /translation="MKIGCPKETKDHEYRVGLIPAAVKAYVNEGHQVLVQRGAGLGSG
                     IMDEEYGAVGARLVEDAAEAWGADMVVKVKEPLPPEYSFLREGQLVFTYFHFAADEGL
                     TRACLERKIIAVAYETVQEKDGSLPLLKPMSEVAGRMAPLVGSYYLARVQGGLGLLPT
                     GVPGVAPAKVLILGGGTVGSNAARVAAGLGADVIILDINVDTLEHLGNIMPPNVHPIF
                     GYGSTFEEWLREADLVVGAVLIPGAKAPRLLKREHFKRMKPGSVFVDVAIDQGGCSET
                     SRPTSHSDPVYVVDQVTHYCVTNMPGAYSRTSTFALNNRTIKYGLKLARQGALAACRA
                     DPALRKGLNLYRGWVTLEPVAEVFGLEECFVPPEEILEKRE"
BASE COUNT          484 a          760 c          754 g          548 t
ORIGIN      
        1 ggatcgtcct tccggcggac cccgggttta gtccggggca gccgccggta ttcgggagag
       61 aggcaagcac ggcgtctttg gccgaaagga cggatattgt gggcccccgg ttggaagagg
      121 ccgggctatg ggcaattgat cctcttcaaa gtccccttgg aaaaatgatt gccaaaggtc
      181 ttggaattga ggtcgtagtc cgttccgact tcgtagaagg tgcctttcat gggcgaggtg
      241 gacgtattca actggatctg catgattccc gtgtcccgcc acccgtcctt gtgcgactgg
      301 gtgccggtga ggttcaaaat cacgtacttg cccgccggca ttccggtccc ccccaggtag
      361 taggggtttt cgtccgctat agttgccttc ccgtggtaca cgatgtagtc cccggacacg
      421 ggagtggctc ttcccgttac ttggaacgag cccttgtcgg gaatggtgac ctcgaagcag
      481 acgggaccgc tcaaacccgc ttcgaccgaa ggaggcgtca caacaaacca cggagtcaac
      541 aacgcgattc ccagaaccaa cgaccatttc ctgaacatac ctttcctttg catcacgcgc
      601 acctttcgtt ttgaagttcc acaggtttcg agcccgatag ccgtcagcat ttcacaagac
      661 gggcatttcg gctaagttcc accgcccggc agatttgcct tcctccgtcc ttccgggcga
      721 tcctatgcaa ccttggttcc actcgtactc cacgaggagg caggatgggc aaggtggctg
      781 ttcacgagac ttggtcaacg tcccccgacc cttcccgcca aggagggaga agactccctc
      841 ttccttggtc ggggagcgaa gaggaccccc gatttcccta tcggttctca ttcccgcttt
      901 tcgaggatct cctcgggagg cacgaagcat tcctccagcc cgaagacttc ggcgacgggt
      961 tcgagggtga cccatccccg gtagaggttc aaacccttcc tgagggcggg gtccgctctg
     1021 caggcggcca aggccccttg ccgggccagc ttcagcccgt acttgatggt tcggttgttg
     1081 agggcgaacg tggacgtcct cgagtaggcc cccggcatgt tggtgacgca gtagtgggta
     1141 acctggtcca ccacgtagac gggatcgctg tgggaggtgg gtcgggacgt ttccgagcac
     1201 cctccctggt cgatggccac gtccacgaaa acggatcccg gtttcatgcg tttgaagtgt
     1261 tcccgtttca agagccgggg ggccttcgcc cccgggatga gcacggcccc gacgaccagg
     1321 tccgcttccc gcagccattc ctcgaaagtg ctcccatatc cgaagatggg atggacgttg
     1381 ggtggcatga tgttgcccag gtgttcgagg gtgtccacgt tgatgtccag gatgatgacg
     1441 tcggctccca gccccgccgc tacccgggcg gcattgctgc ccacggttcc ccctcccagg
     1501 atgagaacct ttgccggggc aacccccggg accccggtag gaagaaggcc cagtcctcct
     1561 tgaacccgcg ccaggtaata ggatccgacc aggggagcca tacgcccggc gacctcgctc
     1621 atgggtttga ggagcggcag ggagccgtcc ttttcctgga ccgtctcgta ggcgacggcg
     1681 atgattttgc gctcgagaca ggcccgggtg agcccctcat ccgccgcgaa atgaaaatag
     1741 gtgaagacca gctgcccctc tcggaggaag gaatactcgg gcggcagcgg ctccttgacc
     1801 ttgaccacca tgtctgcgcc ccaggcttcc gccgcatcct ccaccaggcg tgccccgacg
     1861 gctccatatt cctcgtccat gatgcccgag cccaagccgg cccctcgctg aaccaagact
     1921 tggtgcccct cgttcacata ggcctttacc gccgccggga tgagacccac ccggtattca
     1981 tggtccttcg tctccttggg acatccgatc ttcatagaga aagtctcctc ccggtgaaag
     2041 agcaaccaag gtttcggcat ccaggagcta attagcagaa agtcgctttt ttttctaaag
     2101 aatcttggat ggattgagta taagactcgt gaaaatcccc gggggaatcg gtcccaccga
     2161 cccactcttg aatgatgcgc gctcgacggc tcggaaggga tggagacccg ggagggattt
     2221 tcgcccaatc cagccgtttg ccatgattgg attctcgagg aattgccgcg atgaacctcc
     2281 tcgaaatgtc gttgacattg tcttttgttt tgtggtgtga atacaatctt gtgtctctgt
     2341 tcaagttttt gaggccggtt ggggtggagt gaacgggagc ttacggagtg aggagaaaca
     2401 ggatgggaag acgtttgagt gcaaccgcgg tcctggtcgc ggccctgctt gggctcggct
     2461 cgacgatctg ggcacagacc gtcaaggtcg gattcaacat ccccttgacc ggcgacatcc
     2521 ccaaggtcgg ggagagctcg aagttt
//
LOCUS       DAOOVC010000298         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_22247_length_2541_cov_3.068785, whole genome shotgun sequence.
ACCESSION   DAOOVC010000298 DAOOVC010000000
VERSION     DAOOVC010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22247_length_2541_cov_3.068785"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLM79_18675"
     CDS             <1..576
                     /locus_tag="PLM79_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88386.1"
                     /translation="PFPETRMKDYPHQMSGGMRQRVMIAMAVACRPRLLIADEPTTAL
                     DVTTQAQILELLRKLRDGRGTALLLITHDLGVIASLAEDVVVLYTGRVVEEAPVRELF
                     HRPLHPYTQRLLKSVPGRVSPGAKTRLEAIPGVVPSLLELPRGCKFNTRCPEVFDRCY
                     GEEPPLIPAPNGHPVRCWLYEENAGGKGHGP"
     gene            566..1564
                     /locus_tag="PLM79_18680"
     CDS             566..1564
                     /locus_tag="PLM79_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV88387.1"
                     /translation="MDHEVPKPLILQVRDLKKHYPVTRGFLRREVGRIRAVDGVDFTI
                     CRGETLGLVGESGCGKSTLGRLILRLEEPSGGEVFFQGRNVFALPADQVRALRRQMQI
                     IFQDPYSSLNPRQTVGRIIAEGLVIHGMGSPKERRERVRALMEVVGLRPELLTRYPHE
                     FSGGQRQRIGIARALAVQPALVICDEPLSALDVSIQAQVINLLLDLQERFGLTYLFIS
                     HDLSVVRHLSDRVAVMVHGVIVELAPTEDLFQNPLHPYTQALLEAIPVPDPDAVSRGR
                     TPRTEAPPVPKDAAGCPFSGPCRLQEERCRADRPELREQTPDHWVRCFISREAKRG"
     gene            complement(2053..>2541)
                     /locus_tag="PLM79_18685"
     CDS             complement(2053..>2541)
                     /locus_tag="PLM79_18685"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HOV88388.1"
                     /translation="GLEYIEEILLGDIGYMVGVYVPRLTDDGLTDLRVEYANNAHRVD
                     STPGVWYGHSTYRGGYVHDGMIMGHHMFGDAEDLFVRVSRYLRNDLNLGVDYDFMRRG
                     ITLNPIQEKVNEFGVDLTYDFCGSMRISARYGFQMVDNYDVKEGVDRKNHLFTTAFKW
                     DF"
BASE COUNT          504 a          782 c          758 g          497 t
ORIGIN      
        1 ccgtttcccg agacccggat gaaggactat cctcatcaga tgagcggagg gatgcgccag
       61 agagtcatga tcgccatggc cgtggcctgc cgcccgcggc tgctcattgc cgacgaaccc
      121 accacggccc tggacgtcac cacccaggcc cagatcctcg aattgctgcg aaaactgcga
      181 gatggccggg gaacggccct gctgctgatt acccacgacc tgggagtcat cgcgtccctt
      241 gccgaggacg tggtggtcct gtatacgggc cgagtggtgg aagaagcccc ggtgagagaa
      301 ctgtttcatc gtcctcttca tccgtacacc caaagactcc tgaaatccgt tcccggtcga
      361 gtctccccgg gggcgaaaac ccgcctcgag gccatccccg gagtggtacc cagtctcctg
      421 gaactgcctc gaggatgcaa gttcaatacc cggtgcccgg aagtcttcga tcggtgttac
      481 ggggaagagc ccccgctgat accggctcca aacggccatc ccgtacgatg ctggctgtac
      541 gaagaaaatg caggagggaa ggggcatgga ccatgaggtt cccaagccct tgattttaca
      601 ggttcgggac ctgaaaaaac attaccccgt cactcgcggg tttctccgca gagaggtggg
      661 gcgcattcga gccgtggacg gggtggattt caccatttgc cggggtgaaa ccctggggct
      721 ggtgggggaa agcgggtgcg gaaaatccac cctcggcagg ctcatcctgc gactggagga
      781 gccttcgggc ggggaggtgt ttttccaggg gaggaacgtc tttgccctcc ccgcagacca
      841 ggtccgggcc ttgcgccgac aaatgcagat catcttccaa gatccctatt cctccctgaa
      901 ccctcgccaa acggtgggac gaatcatcgc cgaagggttg gtcatccacg ggatgggaag
      961 cccgaaagaa cggcgggaaa gggtccgggc cctcatggag gtggtcggcc tgagacccga
     1021 gctcctgacc cgctaccccc atgagttcag cggaggacag cgccaaagga tcgggattgc
     1081 ccgagccctg gccgtgcaac ccgccctggt catctgcgac gaacccctct cggccctgga
     1141 cgtgtccatc caggcccagg tcatcaatct gctgctggac ctccaagagc gattcggtct
     1201 cacctacctg ttcatctccc acgatctttc cgtggttcgt cacctgagcg accgggtggc
     1261 ggtcatggtt cacggagtga tcgtggaact ggctcccacg gaagatctct tccaaaaccc
     1321 gctccatccc tatacccagg ccctcctgga agccattccc gttccggacc ccgatgccgt
     1381 ctcccgcggc aggactccgc gaacggaagc tcctcccgtg ccgaaagatg ccgccggctg
     1441 ccccttttcg ggaccctgtc gcctccaaga agaacgatgc cgggcggatc ggcccgaact
     1501 gcgggaacag acacctgacc actgggtccg atgttttatt tctcgggagg cgaaacgcgg
     1561 atagctgcaa cgagccgcgg ggaaatgacc aaggaacaga ggaagagaga ggcgcaaaga
     1621 ggccgcaagg ggggcaacta atcctggaag aaggcaaact tgcggagctc cgatttgtgg
     1681 ggcgccggaa tgagtcggga ggccgtgttt gatcccgcag gccctgagat ggcccggcag
     1741 tcatgcgaca ggtttctcgg cgagggcctt gcaggattca tattcgacaa tcaaggtgtc
     1801 ctctacgaac cggtctcgaa acactcctcg agaagggtgc cgtcttcgtc gtggaccgcc
     1861 ggtgggtatt gcgctgagag cttagatccg agctttcttc atcattgcgc caatccgtcg
     1921 gaccttctcc ggaggcgccg gccgcgcaag gtccgtcgcc gggagaggtc cgtcccacgg
     1981 attccgtccc agggcccaaa gaccggttct tcacccattt ttctttcggt ttcttgcgcc
     2041 tctctgcggt tcctagaagt cccacttgaa ggccgtggtg aacaagtggt tcttgcgatc
     2101 cactccttcc ttcacatcgt agttgtccac catctggaac ccgtatcgcg ctgaaatccg
     2161 catgctcccg cagaaatcgt aggtgaggtc cacaccgaat tcgttgacct tttcctggat
     2221 gggattgagg gtgatgcccc ggcgcatgaa atcgtaatcc actcccaggt tcagatcgtt
     2281 tctcaggtat cggcttaccc gaacgaagag gtcctcggca tccccgaaca tatgatggcc
     2341 catgatcatc ccgtcgtgga cgtagcctcc ccggtacgtg ctgtgaccat accaaacacc
     2401 gggggttgag tcgacgcgat gggcattgtt ggcgtactcg acgcgaaggt ccgtgaggcc
     2461 gtcgtcggtg aggcggggga catagacgcc caccatgtag ccgatgtctc ccagaaggat
     2521 ttcctcgata tactcgagtc c
//
LOCUS       DAOOVC010000299         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_22918_length_2486_cov_7.460304, whole genome shotgun sequence.
ACCESSION   DAOOVC010000299 DAOOVC010000000
VERSION     DAOOVC010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22918_length_2486_cov_7.460304"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1469
                     /gene="murC"
                     /locus_tag="PLM79_18690"
     CDS             18..1469
                     /gene="murC"
                     /locus_tag="PLM79_18690"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOV88389.1"
                     /translation="MDGLKLSHDRIRNVHLLGIGGSGMSGLAALLSELGFEVSGCDMA
                     HSYYMEKLLARKVDCVLGHDPAHLDRYRPDLLIYSSAIPDRNEELIEAGRRGILIARR
                     AEVLSALFNERFGVGIAGTHGKTTTSSMTALILDRAGLKPTVAIGGEVCDIGANARLG
                     AGLPMVAELDESDGSFELFRTNVSVITNVDWDHVDHYRTREDVERAFNRFINNRKKES
                     SVIICGEDPGAARLIEQNRASGLDGSLTTYGWGSGWDWGAVDVNHVRGGGVSFNVLHR
                     GRLKGAMTLKVSGEHNVLNALAACAACSCMNVPFETACEALKAFKGAKRRLQYMGSVG
                     EVDLYDDYGHHPREVSATLRAVRSIFPGRRLVTLFQPHRYTRTAALYRDFADALNQAD
                     RLFLLPIYPADEQPIPGVSSDLIVQAMIEKGSRQVTMCVDIAEAVERIGREAQPEDLV
                     LTLGAGNVNSACELLMSRLEQQEPVPDAMACSA"
     gene            1447..2370
                     /gene="murB"
                     /locus_tag="PLM79_18695"
     CDS             1447..2370
                     /gene="murB"
                     /locus_tag="PLM79_18695"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOV88390.1"
                     /translation="MQWLAALERELNTILEQQVPLAPLTTWKAGGNAETFLAPRNADE
                     AHGAFAFCRREGVPVWMIGGGSNILVHPRGLPGLTLWMGKINTFTLSPQQDGTFLLVA
                     DAGCSIRRLLHFCLANGYSGLEFATGIHGTIGGALICNAGAGQDAVGQKVEWVETVEE
                     DGTLRRWKSSELSFRYRFSSLREGIRLITRCGLKLLKEERQVVLEKAQRFWSLRKAQP
                     HGANTAGCVFKNPGSGKEPAGFLLDRSGCKGMRNGDARVSIIHANFIENRGSASASEI
                     WELIQACRRKVYEASGIWLELEVNLLGGPWD"
BASE COUNT          511 a          685 c          774 g          516 t
ORIGIN      
        1 gaagggagag acggaacatg gatggactca aactgagcca tgaccggatc aggaatgtcc
       61 acctgcttgg aattggcggt tctggaatga gcggactggc cgcgcttctt tccgaactcg
      121 gattcgaggt ttcagggtgt gatatggctc attcctacta catggagaaa ttgctggccc
      181 gcaaagtgga ttgcgtgctc gggcacgacc ccgctcactt ggatcgttac cggccggatc
      241 tcctcattta cagcagtgca attcccgatc gaaacgagga gctgatcgaa gccggcagga
      301 ggggaatcct catcgcgagg cgggcagagg ttctcagcgc tcttttcaac gaacgctttg
      361 gggtcgggat cgcggggact cacggaaaga cgaccacttc ctccatgacc gccctgatcc
      421 tcgatagggc cggtttgaaa cccacggtgg ccatcggcgg ggaagtctgc gatatcggcg
      481 ccaatgcaag gctgggagcc gggcttccca tggttgcaga actggacgag agtgacggtt
      541 ccttcgagct tttccggaca aatgtttcgg tcatcaccaa cgtggactgg gaccacgttg
      601 accactaccg cacgagggaa gatgtcgaaa gggcgttcaa ccgcttcatc aacaaccgga
      661 agaaggaatc atccgttatc atctgcggcg aggatccggg cgcagcgagg ctgatcgagc
      721 agaaccgcgc ttccgggctc gacggctctc ttacgaccta tggctggggt tccgggtggg
      781 attggggagc cgtggacgtc aaccatgttc gggggggcgg agtgtctttc aacgtcctgc
      841 accggggccg tctgaaagga gccatgaccc tgaaggtctc cggagaacac aacgtgttga
      901 acgccctggc agcctgtgct gcctgctcct gcatgaacgt tcccttcgag acggcctgtg
      961 aagccctcaa agccttcaag ggtgcaaaac gccggctgca gtacatgggt tccgtgggag
     1021 aagttgatct ctatgacgat tacgggcatc atccgagaga agtctctgct acccttcgag
     1081 ccgtccggtc gattttcccg ggccgccgcc tggttacttt gttccagccg caccggtaca
     1141 cccggaccgc tgccttgtac agggactttg ctgatgcttt gaaccaggcg gaccggcttt
     1201 tcttgctgcc catctatccc gcggacgaac agccaatccc aggagtctcc tcggatctca
     1261 tcgttcaggc gatgatcgaa aaaggctccc gccaggttac gatgtgcgtg gacatcgcag
     1321 aagccgtcga acggattggc agggaggccc agcctgaaga tctggtcctt acgctgggtg
     1381 ccggcaacgt caattcagcc tgcgaactgc tgatgagccg cctcgaacag caagagcccg
     1441 ttcccgatgc aatggcttgc agcgcttgaa agagaactga ataccatcct tgagcagcaa
     1501 gtccctcttg cgcccctgac cacctggaag gccggaggca atgcagaaac cttcctggcc
     1561 ccgcgcaatg ccgatgaggc acacggggcc tttgcctttt gccgaaggga aggggtgccg
     1621 gtgtggatga tcgggggcgg cagcaatatc ctcgttcacc ccagggggct ccccggcctg
     1681 actctctgga tgggaaaaat caacacgttc accctctcgc cgcaacagga tggcaccttc
     1741 cttcttgtag ccgatgcagg atgttcgatc cgacgcctgc ttcatttctg cctggccaac
     1801 ggttactcgg gcctggagtt cgctaccggc atccacggaa ccatcggggg tgccctgatt
     1861 tgcaacgctg gggcggggca ggatgccgtg gggcagaaag tcgaatgggt ggagacggtg
     1921 gaggaggatg ggacactccg ccggtggaaa tccagcgaac tttccttccg ttaccgtttc
     1981 agttcgcttc gggagggaat tcgcctgatc actcgctgcg ggctcaaact cctgaaggaa
     2041 gaacggcagg tcgtcctgga aaaagctcaa aggttctggt ctctaagaaa ggctcagccg
     2101 cacggagcca acacggcggg gtgcgttttc aagaatccgg gttctggaaa ggaacccgcg
     2161 gggtttttgc ttgaccgctc gggctgcaaa gggatgagga atggggacgc gagggtttcc
     2221 attattcatg cgaatttcat tgagaaccgc ggttccgcca gcgcaagcga gatctgggag
     2281 ctgattcaag cctgcaggcg aaaagtgtac gaagcgagcg gtatctggct ggaactggag
     2341 gtgaaccttc tcggtgggcc gtgggattaa gggtggggct ttaggccgaa aattcctttg
     2401 gcttgccctt tttttcggaa tcctctgcgg gttggaaaac cattaccagc ttctccggct
     2461 ggtcgagctg accgttgagc cgccag
//
LOCUS       DAOOVC010000300         2483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_22959_length_2483_cov_4.033361, whole genome shotgun sequence.
ACCESSION   DAOOVC010000300 DAOOVC010000000
VERSION     DAOOVC010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22959_length_2483_cov_4.033361"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            416..1168
                     /locus_tag="PLM79_18700"
     CDS             416..1168
                     /locus_tag="PLM79_18700"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOV88391.1"
                     /translation="MGAAAYLRVSAPDQAGEDAYGLDAQNHAIQKYCQEHDIDLVAVY
                     EDTQSGAITDRPGLMALLAECKTGKFTKVLVPKLDRLSRDALYCLWIEKELRKNNVEL
                     ISAAEPYRWDDPMQKMMLTIMAAFAEYEKELIKIRLCGGRKAKARKGGYSGGRPCIGY
                     KAKSGSKALLLDDAKAKIVKRVFEIRDSHPDLYLKDIARMLNEEGHTPAKGKKFYPMQ
                     VQRILKKRPFYKGVYLYAGVQAPGQHPVILAY"
     gene            1235..1849
                     /locus_tag="PLM79_18705"
     CDS             1235..1849
                     /locus_tag="PLM79_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88392.1"
                     /translation="MGEIIPFPTGNGPPEIDWLEIEVIRRAKYPRLPRKALALIKRIL
                     ELGPRGNPSLIRLDILHHDDYLPDPLVLMPCKGDLLWVMDLLDRVGGTLACGSVEYAV
                     NRKKKRPPLVQVQGIGESPLEAMKLCIGPEYEHLILTQDWKVLSRDIPECDLMILLIV
                     RRDYQDSGFLGLFDKTTALIFFERLKSGRLLETVFQLPLEECSR"
     gene            1988..>2483
                     /locus_tag="PLM79_18710"
     CDS             1988..>2483
                     /locus_tag="PLM79_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88393.1"
                     /translation="MAAASIHEGAIVVGTLFNEPMRVETVRDNGDGTWIVGLVGMQTE
                     RFRRVALTTRDLEDLKVLDSSLTFDGDGRLLRLGLQAYSLGIAWEFDPYFGLSVSRVD
                     PLPHQLEAVYDYLLKVARVRFLLADDAGAGKTIMSGLLVRELQLRGLAERILIVCPAN
                     LAFQW"
BASE COUNT          589 a          665 c          653 g          576 t
ORIGIN      
        1 gagagggatt cgaaccctcg gttgaagttt cccccaacac tcgcttagca ggcgaagagg
       61 cggggtgttc cggcatgcgc ctctgcctgc aaagtggctc tgagattgac ttcttctgac
      121 cgctacacct cgctagttcg cgctacttct ctgaaaagtc cccccgattt ccccccgatt
      181 ttttttagac cagccacatt caacctctaa gaacaggcta aatttcctca taacattcag
      241 atagttgcaa ggtggtgaaa aattgccctt gtaaccatac gaatttctta gaaaagtttg
      301 taatttttta catcattttg cgccctatta gttattgagg ggcaatgacg tccccctcta
      361 cctctaccca tctaccttct cccaacaaaa ccatttcgct gaaaggagta tcattatggg
      421 tgcagcagca tatctacgtg tgtctgcccc agatcaggcc ggagaagacg cttatggcct
      481 ggacgcgcag aaccatgcca tccagaagta ttgtcaggag cacgatattg acctcgtggc
      541 agtctacgag gatactcagt ctggagcgat caccgacaga cccggattga tggctctact
      601 tgccgagtgc aagaccggga agttcacgaa agtcctcgtc ccaaagctcg accgcctttc
      661 ccgcgatgct ctatactgtc tgtggattga aaaggagttg cggaagaaca acgtggagct
      721 gatttcggca gccgagccct atcgttggga cgatccgatg caaaagatga tgctgacgat
      781 catggcagcg ttcgctgaat acgagaagga gctgatcaag atccggctgt gcgggggacg
      841 aaaagcaaag gcccggaagg gcggctactc tggcggacgg ccctgcattg gctacaaggc
      901 caagagcggc agcaaagcgt tgttgctgga cgatgccaag gctaaaatag tcaagcgagt
      961 tttcgagatt cgggatagtc accccgatct atatctaaaa gacatcgcaa ggatgctcaa
     1021 tgaagagggt catacgcctg ctaaagggaa aaagttctat ccgatgcagg tccagcggat
     1081 tttgaaaaag cgccctttct ataaaggcgt ctacctgtat gccggtgttc aagcgcctgg
     1141 ccagcatccg gtaatcctcg cctactgaac ccccgacccc aaataccatt tccgtccctt
     1201 ccattccaat tcaggctcaa aaaggagcat tctcatggga gaaatcatcc ccttccctac
     1261 gggcaacggt ccgcccgaaa tcgattggct ggagatcgag gtaatccgaa gagcaaaata
     1321 cccgcgactc cctcggaaag cccttgcctt gatcaagagg attttagagc ttggccccag
     1381 agggaaccct tcccttattc gtctcgacat attgcaccat gacgactact tgcccgaccc
     1441 gcttgtactc atgccctgca agggagactt gctatgggtg atggatcttc tggatagggt
     1501 cggtggaacc ttggcgtgtg gtagcgttga atacgcggtg aacaggaaga aaaagagacc
     1561 gccgctggtg caagtccaag gaatcggcga gagcccgctg gaggccatga agctgtgcat
     1621 aggcccggag tacgagcacc tgatcctcac ccaagactgg aaagtcctga gccgggatat
     1681 cccggaatgc gatctgatga tcctccttat cgtaagacga gattaccagg attcggggtt
     1741 cctcgggctc tttgataaaa ctacggcctt gatctttttc gagcggctga agtcgggaag
     1801 gctgctggaa acagtgtttc aactcccgct tgaagaatgc agcaggtgag tggagcaggg
     1861 catcgaccgt tgtttccaag gcttagacgt agcctggctt tgctcgtccg atccggtttc
     1921 atggttgttc catccgctca cctgtggtat caaaaaccat aactaagtaa ccgaggaatc
     1981 tggatttatg gctgctgctt caatacatga aggtgcaatt gtggtcggca ccctcttcaa
     2041 tgagccgatg agggtcgaaa cggtgcgcga taatggcgac gggacttgga ttgtgggcct
     2101 tgtcggcatg cagaccgagc gtttccgaag agttgcgctc acaacccgcg acctggagga
     2161 cttgaaggtc ctcgattcaa gcctcacatt tgacggagat ggacgcctcc ttcggttggg
     2221 ccttcaagca tattctcttg ggatagcctg ggaattcgat ccctacttcg gtctttcggt
     2281 atctcgcgta gatccactgc cgcaccagct cgaagccgtc tacgactacc tactcaaggt
     2341 tgcccgtgtc cgattcctgc tcgccgatga cgccggagcg ggtaaaacaa tcatgtccgg
     2401 tcttcttgtc cgagaactcc agctcagggg actcgccgag cggattctca tcgtctgccc
     2461 ggcgaacctc gcattccaat ggc
//
LOCUS       DAOOVC010000301         2327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_25143_length_2327_cov_4.770687, whole genome shotgun sequence.
ACCESSION   DAOOVC010000301 DAOOVC010000000
VERSION     DAOOVC010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25143_length_2327_cov_4.770687"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLM79_18715"
     CDS             <1..736
                     /locus_tag="PLM79_18715"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOV88394.1"
                     /translation="IQGGSVVGPGSVVGDHVIVDEGASIENSVVLDRTYVGVHTRIQN
                     ALVRKNWMLQIPTMLDVYLADDLILGDLEKGTLSTQAGRLLNLFLALGLLVLTSPLWV
                     VYLLYHFLAPSKKVLDSEKRLCGCGQMSLAGVRPPDTFDLFFFKSRNRLIRKLPGLIN
                     VIRGDLNLVGVSPLTEEELEKLPEEWKTLREKAPLGLFHLWELEKEADLEWEEKRVAD
                     SYYAVTRTAWRDLKVLGKALLATLLD"
     gene            complement(1471..>2327)
                     /locus_tag="PLM79_18720"
     CDS             complement(1471..>2327)
                     /locus_tag="PLM79_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88395.1"
                     /translation="PAAIRESINILEFYAEPASLQHLPPEQRVRIVVVMRPSGTEPKH
                     KNMVKVLAPPRDPARETLDEYMDRINRLSRSALDAAMIASYEASLVEYESRVAEEPGK
                     FAFADLSPEDRLELLRLFPIIVSAEAKLAVYFPLRAYLQREAERLAKLEGEEFRLAFE
                     SVRGKVWSVAPDSSTRGYLTNFNKTNGIEFVEESVRMNLARQLSASSPGDPRTGPIFV
                     QALLWFGPEIGPMTFETLLTARVREARRSLEENDPRVSAEVRRIMNSLAGLYGSNADQ
                     SIRPGTES"
BASE COUNT          427 a          708 c          745 g          447 t
ORIGIN      
        1 catccaagga ggatccgtcg tcgggccggg aagcgtcgtc ggggatcatg tgatcgtcga
       61 cgagggcgcc tccatagaaa acagcgtggt cctggatcgc acctacgttg gggtccacac
      121 gcgaatccaa aacgccctcg ttagaaagaa ctggatgctc cagatcccca ccatgctgga
      181 tgtctacctg gcggacgacc tgatccttgg tgacctcgag aaaggcactc tatccaccca
      241 ggccggtcgg ctcctcaacc tgttcctggc cttgggcctg ctggtcttga cctcgcccct
      301 gtgggtggtc tacctcctct accacttcct ggctccctcg aagaaggtcc tcgattcgga
      361 aaaacggctg tgcggctgcg ggcaaatgag cctggcggga gtcagaccgc ccgatacctt
      421 cgacctcttt ttcttcaaga gccgaaaccg gctgattcga aagcttccgg gattgatcaa
      481 cgtgatccgg ggggacttga acctcgtggg ggtctcgccc cttacggagg aagagctgga
      541 aaagcttccc gaggaatgga agaccctgcg ggagaaggct cccctgggct tgtttcacct
      601 gtgggagctg gaaaaagagg ccgacctcga atgggaggaa aagagggtcg cagacagcta
      661 ctatgccgtc acccggaccg cgtggcgaga cctaaaggtc ctgggcaagg ctcttctggc
      721 aaccctgctg gactgaaggg cggatcgaga aaccggccag gaactcgcgc aaatcgtttc
      781 tctttgaagg tctgcaacgc cgcatcggcg aacgtcgccc ggcaaaacgc ttcgggctcc
      841 aggtggatca cgcggcgaga cacttcggca tgggcttcgg gggattccat cgggaaaatg
      901 taggtccccc ggcgctacgg gaggacggcg gggtatacct caaggggcac cgggtcttcg
      961 gggatttcgg tgcgattttg ctgccccccg gaaatcgtcc tgcaccgact gagctctccc
     1021 aaggtttgaa ccttccgctt gcgtccccgt ctcgtgagga ccccatggac cacgggtgtc
     1081 cacccacaaa tcggcccggt atgtgcgtgc cgcctgacgg tgcccggcct acgtggttgg
     1141 gggtgggcac gacaaagccg ggcccaccct acgcagttgc gtcatgaccc gtagggtggg
     1201 cacctttcgg gtgcccaccg gagacctcct aacgcactga tgggtggggc acccttcggg
     1261 tgcccaccag atcccctgat gtacggatga aattgggcta tgacgggtgc cgcgtgatgg
     1321 tgcacacctt acccgtgttt gcccccgacg ggcttgaggc aggcaactcg tggtgcccgg
     1381 gatgggtcat tcgccgaaac caggtaggcg ttcacgaccc aacgcaccac cccagccttc
     1441 gggatcgatt ctcatttggt gggctcagca tcaggattcg gtgcccggcc ggatgctctg
     1501 atccgcattg ctcccataga ggcccgcgag ggagttcatg atccttctca cctcggccga
     1561 aacccgggga tcgttttctt cgagggatct ccgggcctcg cggacccggg cggtgaggag
     1621 ggtctcgaag gtcatggggc cgatctcggg cccgaaccag aggagggcct ggacgaaaat
     1681 cggccccgtc ctcgggtccc ccgggctcga ggcggaaagc tgccgggcca ggttcatccg
     1741 gaccgactct tccacgaact cgatcccgtt ggtcttattg aagttggtca ggtagccccg
     1801 ggtcgagctg tccggggcga cgctccagac cttcccccgc acgctctcga aggcgaggcg
     1861 gaactcttcc ccctcgagct ttgccaggcg ttccgcttcc ctctgaaggt aggccctcag
     1921 ggggaaatag acggcgagct tcgcttccgc cgaaacgatg atgggaaaga gcctcaggag
     1981 ctcgaggcga tcctcgggag aaagatctgc aaaggcgaac tttccgggtt cttcggccac
     2041 ccgggattcg tattcaacga gggaggcctc gtaggaagcg atcatggccg catcgagggc
     2101 gctccgactg aggcggttga tccggtccat gtactcgtcc agggtctccc gtgccgggtc
     2161 tcggggaggc gccaggacct taaccatgtt cttgtgcttg ggttccgtgc ccgaagggcg
     2221 catgaccacc acaatgcgca cccgctgctc cggagggaga tgctgtaggc tcgccggttc
     2281 ggcgtagaat tcgaggatgt tgatggattc ccgaatggcg gcgggcc
//
LOCUS       DAOOVC010000302         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_25966_length_2275_cov_5.301351, whole genome shotgun sequence.
ACCESSION   DAOOVC010000302 DAOOVC010000000
VERSION     DAOOVC010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25966_length_2275_cov_5.301351"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLM79_18725"
     CDS             complement(<1..208)
                     /locus_tag="PLM79_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOV88396.1"
                     /translation="MDPEAPRPLILEVRDLKKHYPVTRGFLRKEVGCIRAVDGVDFSI
                     HRGETLGLVGESGCGKSTLGRLILR"
     gene            complement(198..1199)
                     /locus_tag="PLM79_18730"
     CDS             complement(198..1199)
                     /locus_tag="PLM79_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOV88397.1"
                     /translation="MAPILLDVQDLQTFFFAEQGVARAVDRVSFRVREGRTVGIVGES
                     GCGKSVTAHSILRLVPEPGKIVGGRVLFDGTDLLSMNRKELEDLRGNRISMIFQEPMT
                     SLNPVFRVQDQILEGIRRHRKISGKEALDRIVDLLREVGIPSPETRMKDYPHQMSGGM
                     RQRVMIAMAVACRPQLLIADEPTTALDVTIQAQILELLRHLRESRGTTLLLITHDLGV
                     IASLADDVVVLYTGRVVEEAPVRELFHHPLHPYTRRLLKSIPGRVSSGAKTRLEAIPG
                     VVPSLLELPRGCKFNTRCPEVFHRCYGEEPPLIPARNGHPVRCWLYEENGGGTGHGP"
     gene            1391..1918
                     /locus_tag="PLM79_18735"
     CDS             1391..1918
                     /locus_tag="PLM79_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOV88398.1"
                     /translation="MGVDVSEGLSASLEDYLEAIYHLEQSNRVARAKDIADQLNVQRA
                     SVTGALKALAGRGLVNYSPYSYITLTPEGRAIARDVIHRHETLKDFFTTALQLSPEQA
                     EANACRIEHSIDPEAIDRLVRFLEFIKICPRIGSDWFTAFARYCRKGIRSSDCETCVE
                     VCLRQISPQEEPPPP"
     gene            complement(2058..>2275)
                     /locus_tag="PLM79_18740"
     CDS             complement(2058..>2275)
                     /locus_tag="PLM79_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88399.1"
                     /translation="ILAHLHQGLDAYERWYGEGNLEGFVRDFEASARHLGSFCRDAYE
                     ESSGMLDFAVRSARRGGKPAERSEVKD"
BASE COUNT          453 a          740 c          660 g          422 t
ORIGIN      
        1 gtcgcaggat gagcctgccg agggtggatt ttccgcaccc gctttccccc accaggccca
       61 gagtctcccc ccggtgaatg gagaaatcca ccccgtccac ggctcgaatg caccccacct
      121 ctttgcgcag aaacccgcgc gtgaccggat aatgtttttt caggtcccga acctccaaaa
      181 tcaagggccg tggcgcctca gggtccatgc cccgtccctc ctccgttttc ttcatagagc
      241 cagcatcgaa cgggatggcc gtttcgagcc ggaatcagcg ggggttcttc cccgtaacac
      301 cgatggaaga cctccgggca ccgggtgttg aacttgcacc ctcgaggcag ttccaggaga
      361 ctgggtacca ctccggggat ggcttcgagg cgggttttcg ctcccgagga gactcgaccg
      421 gggatggatt tcagaagtct tcgggtgtaa ggatgaagag gatggtgaaa cagttccctc
      481 accggggctt cctccaccac tcggcccgta tacaggacca ccacgtcgtc ggcaagggac
      541 gcgatgaccc ccaggtcgtg ggtaatcagc agcagggtcg tcccacggct ctcgcgcagg
      601 tgccgcagca gttcaaggat ctgggcctgg atggtgacgt ccagggccgt ggtgggttcg
      661 tcggcgatga ggagctgcgg acgacaggcg acggccatgg cgatcatgac cctctggcgc
      721 atccctccgc tcatctggtg agggtagtcc ttcattcggg tctcggggga cgggatcccc
      781 acctcccgga gcaggtccac gatgcggtcg agggcttctt tcccggagat ctttcggtgc
      841 cttcggattc cttcgaggat ttggtcctgc acgcggaaga cggggtttag cgaagtcatg
      901 ggttcctgga aaatcatgga aatgcgattg ccccggagat cctccaactc ctttcggttc
      961 atggagagca gatccgtgcc gtcaaagaga acccgtcccc ccacgatctt tcccggctcg
     1021 ggaaccaggc gcaggatgga atgggccgtc acgctcttgc cgcacccgga ctcccccacg
     1081 atccccacgg tccggccttc acggacccgg aaggacaccc ggtccacggc ccgggcaacc
     1141 ccttgctcgg caaaaaagaa ggtctgaagg tcttgtacat ccagcagaat gggagccatc
     1201 ctcggcggtc cgttcaacag ggagacgcgg ccgcaatccg ggccgtccgg tcatgggcgg
     1261 tttccccgat acctatactc gacaacggcc gtcctcatca agggctcaag gaagtccccc
     1321 aatgcaacct tgacgggtcg ggcggaaatc gataaggtaa aaaaaagcct cgaggaaagg
     1381 acaggagcca atgggagtgg atgtttccga aggcctctcg gccagcctgg aagactacct
     1441 cgaagcgatt taccatttgg agcaatccaa tcgagtggct cgggccaagg acatcgcaga
     1501 ccagctaaac gtgcagcgcg cctcggtcac gggggccttg aaggccctgg cgggccgcgg
     1561 gctggtgaat tacagcccgt acagctacat taccttgacc ccggagggtc gagccattgc
     1621 ccgggacgtg atccaccgcc acgaaaccct caaggatttc ttcaccaccg ctctccaact
     1681 cagccccgaa caagcggaag ccaatgcctg ccgaatcgag cactccatcg atcccgaggc
     1741 catcgaccgg ttggttcggt ttctcgagtt catcaagatt tgcccccgca tagggtccga
     1801 ctggttcacc gcctttgccc gttactgccg gaagggaatc cgttcgtccg actgcgaaac
     1861 ctgcgtggaa gtgtgtctga ggcagatcag cccccaagaa gaaccgccgc ccccctagaa
     1921 attctcaccg gacgcattca cccccgcgcc gcccaccgcg ggctgcgcaa accgcgcgaa
     1981 aaccacgggc tgccgcattc gcacgcacca cggcgaacaa ggtccgtatc taaggttcgg
     2041 atctccggga tcgttgttca gtctttcacc tccgacctct ctgcgggctt tcccccccga
     2101 cgggccgacc gtaccgcaaa atcgagcatg cccgaacttt cttcgtaggc atcccggcaa
     2161 aaactcccca ggtggcgggc gctcgcttcg aagtcccgta cgaatccctc gagatttccc
     2221 tccccgtacc agcgttcgta ggcgtccaag ccctggtgca ggtgagccag gatgt
//
LOCUS       DAOOVC010000303         2275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_25967_length_2275_cov_5.081081, whole genome shotgun sequence.
ACCESSION   DAOOVC010000303 DAOOVC010000000
VERSION     DAOOVC010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25967_length_2275_cov_5.081081"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="PLM79_18745"
     CDS             complement(<1..1137)
                     /locus_tag="PLM79_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOV88400.1"
                     /translation="MPKQILLPESELPQQWYNVLPDLPQPLPPPIHPGTLQPIGPEQL
                     APIFPMALIEQEVSQERWIQIPKPVMDVYRLYRPTPLVRAYRLEAAIGTKAKIYYKNE
                     AVSPAGSHKPNTSIPQAYFNKMEGVRRIATETGAGQWGTALAFACKMFGIQCTVYMVR
                     CSYDQKPYRGIMIRAYGAEVYPSPSDRTNFGRRLLETMPDTGGSLGAAISEAIEDAAT
                     HDDTKYSLGSVLNHVLLHQTVMGLETKKQLQLAGEKPDVLIGCVGGGSNFSGFILPFV
                     PEKLAGDKVRFLGIEPQACPTLTRGQYRYDFGDVAQQTPLLKMHTLGHNYFPPPIHAG
                     GLRYHGDAPILCHLVRLGLVDAKAYYQTQVFEAAQLFLQTEGFLP"
     gene            1621..2232
                     /locus_tag="PLM79_18750"
     CDS             1621..2232
                     /locus_tag="PLM79_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOV88401.1"
                     /translation="MEKIVAIYGSPRRNGNSSSLLRRAVQGAREAGADVREFVLRDLR
                     MSPCLEIYRCKETGRCAIRDDFQAVVDELTTCRGLILASPIFFYTVSAHTKILMDRCQ
                     SLWVKKYWIDQVPFGRGEPKRRGLFISVGATKGQKLFDGTLLTVRYFFDVLDMELWRA
                     LLYRGLDFEGDVQKHPEFLDDAYQAGKELVLSLRDRPREQEIP"
BASE COUNT          432 a          643 c          736 g          464 t
ORIGIN      
        1 cgggagaaag ccctcggtct ggagaaagag ctgggccgcc tcgaagacct gggtctggta
       61 gtaggccttg gcgtccacga gccccaggcg aaccaggtga cagaggatgg gggcgtcccc
      121 gtgataacga aggcctcctg cgtgaatggg aggcgggaag taattgtgtc ccagggtgtg
      181 catcttgagg aggggagtct gctgggctac gtccccgaag tcgtatcggt attggcctcg
      241 agtgagggtg ggacaagcct ggggctcgat tcccaggaac cgtaccttgt ctcccgccag
      301 cttctcgggc acgaagggca ggatgaaccc cgaaaagttg ctgccgcctc ccacacaccc
      361 gatgagaaca tcgggctttt cccccgccag ctgaagctgc tttttggtct cgagccccat
      421 aacggtctgg tggagaagca catggttcaa gacgctgccc agggagtatt tggtatcgtc
      481 gtgggtggca gcgtcctcga tggcctcgga gatggccgcc ccgaggctgc cgcccgtgtc
      541 cggcatggtc tcgaggaggc gccgtccaaa attggtcctg tccgagggag acgggtacac
      601 ctcggctccg taggcgcgga tcatgatccc ccggtagggt ttctgatcat agctgcagcg
      661 aaccatgtac accgtgcact ggatcccgaa catcttgcag gcaaaggcca gggccgttcc
      721 ccattggccc gccccggttt ccgtggcaat gcggcggacc ccctccatct tgttgaaata
      781 ggcctgggga atggacgtat tgggcttatg ggacccggcg ggagagacgg cctcgttctt
      841 gtaatagatc ttcgccttgg tcccgatggc cgcttccagg cggtacgccc gcaccagggg
      901 agtggggcgg tagaggcggt agacgtccat gaccggtttg gggatctgga tccagcgttc
      961 ctgggaaacc tcctgctcga tgagggccat ggggaaaatg ggggcgagct gttcgggccc
     1021 gatgggctgg agcgttcccg gatggatggg gggcggcaac ggctgcggca ggtccggcaa
     1081 cacgttgtac cactgctggg gaagctcgct ttcgggcaaa agaatctgtt tgggcataca
     1141 cagacctcct ttgtgctttt cgatgggcta cctggatttg catccgaatc tcgaccggtc
     1201 cgtccggtgc agcgccggtt gccgctcccg gcagggccgc cagtggtttc atcggatggg
     1261 gaaaaagtgc cggggattat ccgctcagcg aaccaagttg ggcagcttcc gtcctggcgg
     1321 tgtggtcgag gaaatgcggt cgaaccgatc gggggcaatg ccgctttgcg ggaaatctcg
     1381 tccaaaggca ggctacgaat accatgattt gctcccttcc acaacctctc ttgtgacaca
     1441 aatcactcaa tctttccccc cgccgttcca ttttctcgca agtcttttgg gattcgtgac
     1501 ggtacaattg gaaagcacgg agatgagagt ttcccgacgg ctgagatggg gggcgccggg
     1561 cggcagggcc gattcatccg caaagccggc gcaacggaat ggatgaggag aaggacttct
     1621 atggaaaaga tcgtggcgat ttacggcagt ccccggcgaa acggcaattc atccagcctg
     1681 ttgcgccggg cggttcaggg ggctcgggag gcgggggccg atgtgcggga attcgtgctc
     1741 cgggatctgc gcatgtctcc ctgcctggaa atctaccgct gcaaagagac ggggcgatgc
     1801 gccatccggg acgacttcca ggccgtggtc gacgaactca ccacatgtcg ggggctgatc
     1861 ctcgcctccc ccattttctt ctatacggta agcgctcata cgaaaatcct catggatcga
     1921 tgccagtccc tgtgggtgaa gaagtactgg atcgatcagg tcccgttcgg ccgcggggaa
     1981 cccaaacgca ggggcctttt catctccgtg ggcgccacca aggggcagaa gctcttcgat
     2041 ggcaccctgc tcaccgttcg ctatttcttt gacgtgctcg acatggagct ctggcgagcc
     2101 ctcctttacc ggggactcga tttcgaaggc gatgtccaaa aacatcccga attcctcgat
     2161 gatgcctatc aggcgggaaa agagctggtc ctctccctcc gagaccggcc ccgggagcag
     2221 gaaatcccgt gaagcgtctt ttttccccgg ggaaaaaaga cgcttcacgg gattt
//
LOCUS       DAOOVC010000304         2265 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_26139_length_2265_cov_6.563348, whole genome shotgun sequence.
ACCESSION   DAOOVC010000304 DAOOVC010000000
VERSION     DAOOVC010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26139_length_2265_cov_6.563348"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(331..418)
                     /locus_tag="PLM79_18755"
     tRNA            complement(331..418)
                     /locus_tag="PLM79_18755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Leu,seq:taa)
     gene            957..2138
                     /locus_tag="PLM79_18760"
     CDS             957..2138
                     /locus_tag="PLM79_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOV88402.1"
                     /translation="MKRWKHWIGPALLIVPLSWAMMIFLGTSAPCEAKEPYKVGAVFS
                     ITGGGSFLGDPEKKTVEMIRDEINRAGGINGHPLELVIYDDESDSTKCNLAVKKLIKK
                     DKVPVIIGPSTSGESLAVVGVAEEGRVPLISCAASYKIVTPIESRKWVFKVAGSDSHV
                     VGKLYDHMKAKGITKVALMTASTGFGASGREELLRIAPDYGITIVADERYGPQDTDLS
                     PQLTKIRGTQPQAVVNWSTGPTQILAVKNWRDLGMESIPLYQSHGFGNRKNIELAGKA
                     SEGVLLALARVNVGPLLPDTRPQKKVIMEYTKAYEERYKEPVSSFGGHGWDSLHLAVA
                     ALKAVGPDPAKIRDFLENTQGFVGMHGIFNFTPQDHCGLSKDDLEMVVVKNGDWALVE
                     F"
BASE COUNT          471 a          669 c          693 g          432 t
ORIGIN      
        1 cctgtgggtc agtgtcggac tgacagagag gaagtgtctg actcgcctgg cagccgattg
       61 ccctttgttt gacaggcatg tatggggtgg agccgctttt tcggcgaaag tccatggaac
      121 ttgaaagctg ttcgcacaaa agtctaccac ttgctggtgg cacaacactt ttccggaagg
      181 tgaaggttcc ccgtctcatg gtccctgtgc atcgctcgca gtaaatccga gatgagcaga
      241 tttgacggcc tcgtattgct caaaggttgg gaaacggttg ggaaaaaacg cccttttcgg
      301 attctctcgt tttcactgct ctccatagat tggtgcctgg ggcgaggatc gaactcgcac
      361 gcccgcgagg ggcaaaggat tttaagtcct ttgtgtctac ccgttccacc acccaggccc
      421 cggggaagac ctctatcaca aacccaaaaa acctgacaag ggaattccgg gaagacccaa
      481 cgggcctgcc gctttctggt tgacagggtc gtcgcccctc ccctatataa tttctgtcca
      541 tataatcctg aatagccgca gacctgccgc gagctgcgca atcctgagcc ggaaagccgc
      601 ctttccgatc tttccccaag gcccttgcgg gttaccccgg cggccccgtg tccggtgtac
      661 gttcccggta gccgaggccg gcgaaagggt tcagccgttg gggagttcat tctcccctcg
      721 agggagcagg ggggaatcca tgggcccgtg cccccgtatc tctttccggc ggattcgaag
      781 acaaggcgga aaggatgaac cccacagtcg cggcgtgccg gcccgcaggg gccgtgggac
      841 acatcccttg cggcatgttc ccggccgttt gacgggtctc cctttgccgt ggccttgaga
      901 ccacggttct tccttatccc aaaaccctca ccgaactacg aaggaaagga gtcgtcatga
      961 agcgatggaa acattggatc ggtccggccc tcctcatcgt ccccctgtcc tgggccatga
     1021 tgattttcct cgggacatcc gcaccctgcg aggccaagga accctacaag gtcggggccg
     1081 tcttctccat taccggcgga ggctctttcc tgggagaccc ggagaagaaa acggtggaga
     1141 tgatccggga tgaaatcaac cgggcgggag gcatcaacgg ccacccgttg gagctggtga
     1201 tttatgacga cgagagcgat tccaccaagt gcaacctggc ggtgaaaaag ctcatcaaga
     1261 aagacaaggt gccggtgatc atcggtccca gcaccagcgg ggaaagcctt gccgtggtgg
     1321 gggtggccga agagggccgg gtgcccttga tttcctgcgc ggccagctac aagatcgtca
     1381 cccccatcga aagccgaaaa tgggtcttca aagtggcggg ctccgacagc cacgtggtgg
     1441 ggaaacttta cgatcacatg aaagccaagg ggatcaccaa ggtggccctc atgaccgctt
     1501 ccaccggatt tggagccagc ggccgggagg agctcctgag gatcgctccc gactacggga
     1561 tcaccatcgt ggccgacgag cgctacggtc cccaggacac ggacctgtcc ccccagttga
     1621 ccaagatccg gggaacccag ccccaggccg tcgtcaactg gtccacgggt ccgacccaaa
     1681 tcctggcggt gaagaattgg cgcgacctgg gcatggaatc catccccctc tatcagagcc
     1741 acgggtttgg gaacaggaag aacatcgagc ttgcgggcaa ggcctccgag ggagtcctcc
     1801 tggccctggc ccgggtgaac gtcggtcccc ttctgcccga cacccggcct cagaagaagg
     1861 tcatcatgga atacaccaaa gcctacgagg agcgttacaa ggaaccggtt tcctccttcg
     1921 gcggtcacgg ttgggattcc ctccacctgg ccgtggcggc cctcaaggcc gtgggtcccg
     1981 accccgccaa gatccgggat ttcctggaaa acacccaggg cttcgtgggc atgcacggga
     2041 tcttcaactt cactccccag gaccattgcg gcctcagcaa ggacgacctc gaaatggtgg
     2101 tggtgaagaa cggggactgg gcgctggtgg agttctagat cgaagggccg gaggctggga
     2161 aggcgagggg ccgactgccg ggggcaggga ggccggaggc gatgggctcg atgcgggata
     2221 ggcggaatgg gaattgattt ttcccccgat tccggtcata aaagt
//
LOCUS       DAOOVC010000305         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_26301_length_2256_cov_4.234439, whole genome shotgun sequence.
ACCESSION   DAOOVC010000305 DAOOVC010000000
VERSION     DAOOVC010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26301_length_2256_cov_4.234439"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1484
                     /locus_tag="PLM79_18765"
     CDS             12..1484
                     /locus_tag="PLM79_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOV88403.1"
                     /translation="MGFDKPRLMIAALRGGSGKTLVTMGLAAAWNRLNGLRVVPFKKG
                     PDYIDAGWLSMAAGHPCYNLDPFLMEPADMLGSFLERIQGADVALIEGNRGLFDGVDR
                     RGSYSSAELAKLLGTPVVLLVDATKVTRTAAAMVLGCRQLDPEVPLAGVILNRVAGKR
                     HEQVLRGSIQDACGIPVLGVVPKDPGILFPERHMGLVPPQESPNVSEALDCVAHKIAD
                     CLDLQTLLHLARTVKPLGWPVGPGHAEKSPKTGDPVVVGIVRDAAFQFYYPENLEALQ
                     EKGAVLVEISSFARQPLPPLDALYIGGGFPETHLQVLADNEDFRASLGRRIEEGLPVY
                     AECGGLMFLCREISFEGRSYPMVGTFPYPVVLEKKPQGHGYTVMECVNHNPFLAEGTT
                     LKGHEFHYSQVLLGPNPSVSFIFRLKKGHGIVTGWDGLCYKNALASYSHIHAVGNDSW
                     AEGIVRAARRYQSERMNGSVDRSADPDMRLSGACGYSTSL"
     gene            1500..1817
                     /locus_tag="PLM79_18770"
     CDS             1500..1817
                     /locus_tag="PLM79_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HOV88404.1"
                     /translation="MAQIEFKGKAFEVDEDGFIASFDQWCPEWVDYVKTSEGISELTE
                     EHWKVIHVLQDYYKKHGIAPMVRILTKVTGYKLKYIYELFPSGPGKGACKMAGLPKPT
                     GCV"
BASE COUNT          461 a          619 c          702 g          474 t
ORIGIN      
        1 agagtatcgc gatgggtttt gacaagccgc gcctgatgat agccgccctg cggggcggat
       61 cgggaaagac cctggtgacc atggggctgg cggcggcgtg gaaccgcctc aacgggcttc
      121 gggtggtccc cttcaagaag gggccggact acatcgatgc cgggtggctc tccatggccg
      181 ccgggcatcc gtgttacaac ctggatccct tcctcatgga acccgccgac atgctgggtt
      241 cttttctgga gcgcattcaa ggggccgatg tggccctcat cgagggcaac cgcggcctct
      301 ttgacggagt ggaccgtcga ggaagttatt cctcggcgga actggccaag cttctgggaa
      361 cgccggtggt gctcctggtg gatgccacca aggtgacgcg gaccgccgcc gccatggttt
      421 tgggatgccg acagctggat cccgaagtgc ccctggcggg ggtgatcctg aaccgggtgg
      481 cgggcaagcg ccatgagcag gtgctgcgcg gatccatcca ggacgcttgc gggattcccg
      541 tactgggagt cgtgcccaag gacccgggga ttctttttcc cgagcgccac atgggtcttg
      601 ttcctcccca ggaatcccca aatgtctccg aagcgttgga ctgtgtggcg cacaagattg
      661 ctgattgcct cgacctgcaa acccttttgc acctcgcgag aaccgtgaag cctctcggtt
      721 ggccggttgg tccgggccat gccgagaaat ccccaaagac gggtgacccc gttgtcgtag
      781 gaatcgtccg agacgctgcg tttcagttct actatcctga aaatctggag gccctccaag
      841 agaagggggc cgttcttgtg gaaatctcca gctttgcccg tcagccgctt ccccccctgg
      901 acgccctcta catcgggggc ggattcccgg aaacccacct gcaggtcctg gccgacaacg
      961 aagactttcg agcctccctt gggcggcgaa tcgaagaagg cctgccggtg tacgcggaat
     1021 gcggaggtct gatgttcttg tgccgggaga tttccttcga gggccggtcc tatcccatgg
     1081 tgggcacttt cccatatccc gtggtattgg aaaaaaagcc gcagggtcac ggatacacgg
     1141 tcatggaatg tgtgaaccac aatccatttc ttgcagaagg aacgaccctc aagggtcatg
     1201 agtttcatta ttcccaggtc ctcttggggc cgaacccatc ggtctcgttc atttttcggc
     1261 tgaaaaaagg acatggaata gtgacaggtt gggacggact gtgctataaa aacgccttgg
     1321 cgagttattc gcacatccac gcagtcggca atgattcatg ggcagaaggc attgtgcgcg
     1381 ctgcccgacg gtatcagtcc gaaaggatga acggatcggt ggatcgcagt gcagatccgg
     1441 acatgaggct ttctggagcc tgcggatatt caacatcact ataaaaggag gaagtgtcca
     1501 tggcacaaat cgaattcaag gggaaagcgt ttgaggtgga tgaagatgga ttcatcgcca
     1561 gcttcgatca gtggtgtccc gaatgggtcg attacgtgaa gacttcggaa ggaatcagtg
     1621 aacttaccga agagcactgg aaggtcatcc acgtgctcca ggactactac aagaaacacg
     1681 ggattgcccc catggtccgg atcctcacca aggtgaccgg ctacaagctc aagtacatct
     1741 atgagctgtt cccctcggga ccgggcaagg gagcctgtaa gatggcggga ctgcccaagc
     1801 cgaccggttg cgtgtaggcg cggttggctg attggtcggt tggttcgtcg ggttggcgac
     1861 gggaagcact gtgctgtccg ccgccatgcc ccaaagatcg tgtagaagcg ctggggctgt
     1921 ccccatggag gagacggccc cagcgagccg ttccccttac cgtcccgttc ccgacgccgg
     1981 gctttccgga tgcaacgggc gacaaaacgt gggggctgcg gatcgcccat gcctgtaact
     2041 ttccatcccg tgatgcaccc gtgcaatcat ccatgacttt cattcccgag tgactcgtcg
     2101 gtgagttttg gacgccgaac gaggtcgagc aaacattggt ttgatgatac cgaatagggc
     2161 ttgacaatcc caaagggatg gggttaccta gattccagtt tctgcggcta agtggggagg
     2221 aagaggctac ccctcggtaa gggcatatcg ggtgat
//
LOCUS       DAOOVC010000306         2211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_27060_length_2211_cov_3.990260, whole genome shotgun sequence.
ACCESSION   DAOOVC010000306 DAOOVC010000000
VERSION     DAOOVC010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27060_length_2211_cov_3.990260"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="holB"
                     /locus_tag="PLM79_18775"
     CDS             complement(<1..598)
                     /gene="holB"
                     /locus_tag="PLM79_18775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOV88405.1"
                     /translation="MSFADLPPQPRVQRFLQQLVRKGQVPHAMMFCGMGGVGKAQLAK
                     EFSKLLNCADPKDLEACGRCAPCHKVDVGTHPDLLWIQPETSIIKIDQVREVKQRLRF
                     RPHEARRRVIVIDGAHNLKDEAGNALLKILEDPPPRNLFLLLTPESRMVLATLVSRCC
                     RVQFQPLPDEWIAGYLANTFGLPADRARSLARRSDGSLD"
     gene            complement(657..1313)
                     /gene="tmk"
                     /locus_tag="PLM79_18780"
     CDS             complement(657..1313)
                     /gene="tmk"
                     /locus_tag="PLM79_18780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOV88406.1"
                     /translation="MNPPRTPPKFISFEGIDGAGKTTLAGELSRLLSRAEIDHIVTSE
                     PGGTGLGERLREILLGAGVLEIDPWAECLLYAAARAQHVREVIVPALEDGRWVVSDRF
                     ADATLAYQGYGRGLDVERLRSLHQWIARGIWPHCTVLLDCDVAVAYERRKTRGAHADR
                     LERMDPAFHERVRRGYLALAEAEPHRYLTLDAAVSPERLREEFRRKLCLRRDLFPEGL
                     "
     gene            complement(1300..>2211)
                     /locus_tag="PLM79_18785"
     CDS             complement(1300..>2211)
                     /locus_tag="PLM79_18785"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOV88407.1"
                     /translation="RVWENAEEVCNAVPVKTAVFLRGRSEKYREELQLNIREIRPLET
                     DEVDASDFLPACPVEPGVLFEGLQKILGTVKRRSLRQLSTAFLSDQSLMKRFRIAPAA
                     KSMHHAYLGGLLEHTLGVTRLVSEVCELYPVLDRDQLLVGAFLHDIGKIDEFVYDLFI
                     DYSDAGRLLGHMVLGVEILEKKSRTLKTPHHEEMRALKHLILSHHGEAEFGAVKPPMT
                     REAFVLHAADDLDAKLNFLGRILEAPGDPGQLWTGYQPLFDRFFYRGAPFPAERKPSP
                     GKAGPEEGITQLSLWADNRQKSPHESS"
BASE COUNT          426 a          700 c          676 g          409 t
ORIGIN      
        1 ggtccaggct cccgtcggac cttcgagcca gggaacgggc gcgatccgcg ggcaacccga
       61 aggtatttgc caggtacccg gcgatccatt cgtcgggcaa cggctggaac tgcaccctac
      121 agcagcggga aaccagggtg gccaggacca tccgggattc cggggtgagc agaagaaaga
      181 gattgcgcgg cggagggtct tccaggatct tgaggagcgc gttcccggcc tcgtccttca
      241 agttgtgggc tccgtcaatg acgatgacgc gccgacgggc ctcgtggggc cgaaagcgaa
      301 ggcgctgttt gacctcacgg acttgatcga tcttgatgat cgaggtttcc ggctgaatcc
      361 agagcaggtc cgggtgggtc ccgacgtcga ccttatggca gggagcgcac cggccgcagg
      421 cttccaggtc tttgggatcg gcacagttga ggagcttgga aaattccttt gccagttgag
      481 ccttcccgac tccgcccatg ccgcaaaaca tcatggcgtg gggtacctga cccttacgca
      541 ccagttgctg caagaaccgc tggacgcgcg gctggggggg caggtcggca aaggacatgg
      601 atgaacctct ccacaagcgt ttcggcaaaa gggaacgccg cccggaggcc ctcccgctac
      661 agtccctcgg ggaaaaggtc gcggcgcagg cacagcttcc ggcggaactc ctcacggagc
      721 cgttccgggg aaaccgcggc gtccaaggtc aggtaccggt gcggttcggc ttcggcaaga
      781 gccaggtatc cccgtcgcac tctttcgtga aacgccgggt ccatccgttc caggcgatcc
      841 gcatgggcgc cccgggtttt tcttcgctca taggccaccg ccacatcgca gtcaagcaat
      901 accgtgcaat gaggccaaat tcctcgggcg atccactggt ggagagatct cagacgttcc
      961 acatccaggc ctcgtccgta gccttggtac gccagggtgg cgtccgcaaa acggtcggac
     1021 accacccacc gcccgtcctc gagggccggg acaatcacct cccgaacatg ctgggcccgg
     1081 gcggcggcat aaaggaggca ttccgcccag ggatcgattt ccaaaacacc cgctcccagg
     1141 agaatctccc gcagtcgctc tcccagcccg gtcccccccg gctcggaagt gacaatgtgg
     1201 tcgatctccg cccgggacag cagccgactc agctctcccg ccagggtggt cttccccgcg
     1261 ccgtcgatcc cttcaaagga aatgaacttt gggggtgtcc taggaggatt catgaggtga
     1321 cttctgccga ttgtccgccc agagactcaa ctgggtgatc ccttcctcgg ggcctgcctt
     1381 gcccggggac ggctttcttt ctgcaggaaa cggggccccc cgataaaaga accgatcaaa
     1441 aaggggctga tagccggtcc acagttggcc ggggtccccg ggggcttcga ggatgcggcc
     1501 caggaagttc aacttcgcat ccaggtcgtc cgccgcatgg agcacgaagg cctcccgggt
     1561 catgggcggc tttacggcgc caaattccgc ttccccgtgg tggctcaaaa taaggtgctt
     1621 cagtgcccgc atctcttcgt ggtgaggtgt cttcagagtg cggctctttt tctcgaggat
     1681 ctccacgccc aggaccatgt gtcccagcag ccgtcccgca tccgaataat cgatgaagag
     1741 atcgtagacg aattcgtcaa tctttccgat gtcgtggagg aatgcaccca ccaagagctg
     1801 atcccggtcc aggacggggt agagctcaca cacctccgac accagccggg tgacccccag
     1861 ggtatgttcc aacaatcctc ccaggtaggc gtggtgcatg gatttggccg ccggagcgat
     1921 ccgaaatcgt ttcatgaggg attgatccga aaggaaggcg gtgctcagtt gtcgcagaga
     1981 acggcgcttc accgtgccca ggattttttg gagcccctcg aacaagaccc cgggttccac
     2041 gggacacgcg ggcaaaaagt ccgaggcatc cacctcgtcc gtctcgaggg ggcggatctc
     2101 ccggatgttc agttggagct cttcccggta cttctcgctg cgccctcgca ggaaaaccgc
     2161 agtcttcacc gggaccgcat tgcagacctc ttcggcgttc tcccacaccc g
//
LOCUS       DAOOVC010000307         2157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_28064_length_2157_cov_3.835871, whole genome shotgun sequence.
ACCESSION   DAOOVC010000307 DAOOVC010000000
VERSION     DAOOVC010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28064_length_2157_cov_3.835871"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="miaB"
                     /locus_tag="PLM79_18790"
     CDS             <1..1226
                     /gene="miaB"
                     /locus_tag="PLM79_18790"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOV88408.1"
                     /translation="LNTCAVREKPEQKVFSFLGRLRRLKARNPALKILVGGCMAQQLG
                     KQLLDRFDTVDLVLGTRAIGNARELLERVRETGSRLAYLPEEEDFEDPTCWIHAGATD
                     SVTALITIMQGCDNFCTYCIVPYVRGRERSRPPERIVLEAKWAVESGVREVVLLGQNV
                     NSYGHGLSPRVSFVELLGRVQEVPGLSRIRFTTSHPKDLTESLMKAFVELPALCPHLH
                     LPFQSGSDRVLRAMNRGYTAAQYAQKIYQLREICPEIALSADVMVGFPGETEKDFQDT
                     LELMESIRFDSLFSFIYSDRPFTRAAQFGDKVDSATKSRRLSQLQELQARITLEKNRA
                     EVGAVREVLVEGESKASNGQFTGRTPQNRVVNFVGSRQWIGRLIPVRIVEAYSHSLKG
                     EAVSRGGEGFFQGGD"
     gene            1581..2078
                     /locus_tag="PLM79_18795"
     CDS             1581..2078
                     /locus_tag="PLM79_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOV88409.1"
                     /translation="MYREMKVAGLVMDPQSNTPIVLLKDLHSEESLPIWIGLLEATSI
                     ATELEKIQFPRPMTHDLMKNCLDHFKIRVERIEVCDLRNNTYFALIYLRENDRVTSID
                     ARPSDAIAIALRANAPIFVNEEVLSKSQKMEGGGQTVFDKENQEKWAEILNSLDPEAF
                     GKYKM"
BASE COUNT          449 a          626 c          654 g          428 t
ORIGIN      
        1 ttttgaacac ctgcgcggtc cgggagaaac cggaacaaaa ggtgttctcc ttcttgggca
       61 gactgcgccg cctcaaggcc cggaatcccg ccctcaagat cctggtgggc ggttgcatgg
      121 cccagcagct gggaaagcag ctcctcgatc ggttcgatac ggtggacctg gtgttgggaa
      181 cccgggccat cggcaatgcc cgagaactct tggaacgagt tcgggaaacg ggctcccggt
      241 tggcctacct tcccgaggaa gaggatttcg aggacccgac ctgctggatc catgccgggg
      301 caacggactc ggttaccgcc ctgatcacca tcatgcaggg atgcgacaat ttctgcacct
      361 attgcattgt cccttacgtg cgcgggcggg agcgaagtcg gcctccggaa cggattgtcc
      421 tggaagcaaa gtgggcggtg gagtcggggg ttcgcgaagt agtgctcttg ggacagaacg
      481 tgaactctta cggtcacggc ctgtcccccc gggtttcctt cgtcgaactc ctgggtcgag
      541 tgcaggaggt ccccggcctt tcccggattc gtttcaccac gtctcatccc aaggacctca
      601 ccgaatcgct catgaaggcc tttgtcgagc tccccgccct ctgtcctcac ctgcacctgc
      661 cgtttcagag cggctccgac cgggtccttc gcgccatgaa ccgaggctat acggcggccc
      721 aatacgccca aaagatatac caactccgcg agatctgccc cgagatcgcc ttgtctgcgg
      781 acgtcatggt cgggtttccg ggggaaaccg agaaggactt ccaggacacc ctcgaactca
      841 tggaatcgat tcgattcgat tccctgtttt ccttcatcta ttccgatcgg cctttcaccc
      901 gggccgccca gttcggagac aaggtggatt cggcaaccaa atcccggcgg ctttcccaat
      961 tgcaggagct ccaggcacga attacgttgg agaagaatcg ggccgaggtg ggggccgttc
     1021 gcgaggtgct ggtggaggga gagagtaaag cctccaacgg ccagttcaca ggccggactc
     1081 cccagaaccg ggtagtcaac tttgtcggct ctcgccaatg gatcggccga ctcatccccg
     1141 taaggatcgt ggaggcgtat tcccattccc tgaaggggga agcggtttca cggggcggag
     1201 agggcttttt tcaagggggc gattgagctt tggccttgaa actttccgtg gttgagctgc
     1261 ttgggcttcc acccgggggg gcgtccgaaa atttagagat acgatgccaa agagctgaca
     1321 cttgaaggcc aagagcgaat cgctccattc agatcttttt caagacatct tttcccggcg
     1381 ggggaagcct ggtattctcc cccggcaggg agaaggttga aaggagccgg gcccaacgga
     1441 ggctccccgc ggaaactacc cgtgccggaa gtccggtgcc cttacggaca tgaggcgtgg
     1501 ccgctgggaa ggcgcatctc ccagaacccg aaaccggtgc ccttacggac atgaggcgtg
     1561 ggcagcagga aggagaaggc atgtatcgag aaatgaaggt ggctgggttg gtcatggacc
     1621 cccaaagcaa cactcccatc gttctcctca aggaccttca cagcgaggag tcccttccca
     1681 tatggatcgg tcttctggaa gccacttcca ttgccacgga gctcgagaag atccaattcc
     1741 cgcgtcccat gacacacgac ctcatgaaga attgtctgga tcacttcaag atccgggtcg
     1801 aacgcatcga ggtttgcgat ctgcgcaaca acacctattt tgctctcatc tacctgcgcg
     1861 aaaacgaccg cgtgacttcc atcgacgccc ggcccagcga cgccattgcc attgcattgc
     1921 gggccaacgc tcccattttt gtgaacgagg aagtcctgtc caagtcgcag aagatggagg
     1981 gcggagggca gacggtgttc gacaaggaaa accaggaaaa gtgggccgag atcctcaaca
     2041 gcctggaccc ggaggccttc ggcaagtaca agatgtaggg tggggctttc gcggcaaacc
     2101 acgacaggac ggcgttccgg gacctgtcgt acggggggat gtcgggtctg caggggt
//
LOCUS       DAOOVC010000308         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_28620_length_2128_cov_2.813314, whole genome shotgun sequence.
ACCESSION   DAOOVC010000308 DAOOVC010000000
VERSION     DAOOVC010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28620_length_2128_cov_2.813314"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..2113
                     /locus_tag="PLM79_18800"
     CDS             392..2113
                     /locus_tag="PLM79_18800"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOV88410.1"
                     /translation="MRDATFFTQPVPAWQRRYETLRAFFVDRLPARLVAERFGFTPGY
                     VRLLCHQFRHGKIDLAEIPGEGKSVRRKVGSETRGKIVAWRKQGLSAGEIVELLSEEG
                     GELSVRTVERVLAEEGFSKLPRRTRLKLGFTVRGAQVPQRSQLLASVGELEGQRLEST
                     GAGIFLFAPFMAQLGIEQVIQAAGLPGTKTIAALNYLLSFLALKLLGSERYAHVGEHA
                     FDPVLGLFAGLNVLPKCTALSAYSYSLDEIHLTRLQQAFVQRSSRLGLYEGTTINLDF
                     HTVPHYGDESVLEEHWAGARGKVMKGALTLFAQDAQTKLMLYTAADIRRKEADDQVLA
                     FLSFWKRVRRGIKPTLIFDSKFTTYENLSKLDRQGVKFITLRRRGHKLINEVEKIDAW
                     KRITIAHPKRKFPHPLVHESEIVLSGYAGTVRQIIMRGNGHEKPAFIITNDREAPVEL
                     IVGNYARRWRVENGIAEAVKFFHLNALSSPILIKVHFDVIMTMIADTLYARLSQNLRG
                     FEACDAPKLYRDFVKGKGEVLIKDGRVLVKYPRRAHNPILRAVPRQRLPQKIPWLDDV
                     PISFDFA"
BASE COUNT          417 a          669 c          586 g          456 t
ORIGIN      
        1 tgtgccggcc acaccgatgg ctgccggttt tcgttttagg ccataacgtc tgtaacaccg
       61 attttcgcgg cgcttttgtc agtaacaccg ccgcgtcgat atttccatag atgaatccga
      121 taagtgagga ttcatgagga atcgagatct tctgcaccac ggagccgctc actttgtgtt
      181 attcaatatg acgcttaggc cgatgcaccg cctgcagtca aaagacgaca agcggtacca
      241 cggctggtgc cgcaccgacg ccgtaagacg tggaaatgca aagcatttgt gtgtcagtac
      301 gctactcctc gcggccctct cccgagttcc ggctttttta gttgtatgtt tttgtagtga
      361 cacatgtaac cgtcagtgta ttataacatg gatgcgagac gccaccttct tcactcaacc
      421 ggtcccggcc tggcagcgtc gctatgagac gcttcgcgcc ttcttcgtcg atcggctgcc
      481 cgcccgcctc gtggccgagc gattcggctt cacgccaggc tacgtacgcc tgctctgcca
      541 tcagttccga cacgggaaga tcgacttggc ggaaatcccc ggggagggca agtccgtccg
      601 tcgcaaggta ggctccgaaa cccgcgggaa gatcgttgcc tggcgaaaac aaggtctttc
      661 tgcgggggag atcgtcgaac tgctcagtga agagggaggg gaactttcgg tacgtaccgt
      721 ggaacgggtc cttgccgagg aggggttttc caagcttccc cggcgtaccc gtctcaagct
      781 cggcttcacc gtccgagggg cgcaagtgcc gcagcgttcc caactcctcg cttcggtcgg
      841 cgaactcgaa gggcaacgcc ttgaatccac cggcgccggc atcttcctgt tcgctccctt
      901 catggctcag ctcggtatcg agcaagtgat ccaggcggcg ggcctgcccg gcaccaagac
      961 gattgcggcc ctcaattact tgctctcctt cctggccctg aaactgctgg gcagcgaacg
     1021 ctacgctcat gtcggggagc atgcgttcga tccggtcctc gggcttttcg ccgggctcaa
     1081 tgtccttccc aagtgcacgg ctctgtccgc ctactcctac tcgctcgacg agatccacct
     1141 tacccggctg cagcaggcct tcgtgcagcg ctcctcccgt ctgggtctct acgagggaac
     1201 cacaatcaac ttggacttcc ataccgttcc ccactacggc gatgagtcgg tgctcgagga
     1261 gcactgggcc ggggccaggg gcaaggtcat gaagggagct ctcactctct tcgctcagga
     1321 cgcgcagacc aagctgatgc tctacacggc ggcggatatc cgcagaaagg aagccgacga
     1381 ccaagtgctg gcctttctct ccttctggaa acgggtgcgg cgcggtatca agccgactct
     1441 gatcttcgat tccaagttca ccacctacga gaacctctcg aagctcgatc gccagggggt
     1501 gaagttcatc acccttcgcc gtcgggggca caagctcatc aacgaagtcg agaagatcga
     1561 cgcttggaaa cgcatcacca ttgcccaccc gaagcgcaaa ttcccccatc ccctggttca
     1621 tgaatccgag atcgtcctga gcggctatgc tggaaccgtc cgccagatca tcatgcgtgg
     1681 caacggccat gagaagccgg ctttcatcat caccaacgac cgcgaagccc cggtcgagct
     1741 catcgttggc aactatgccc gtcgctggag ggtggaaaac ggcattgccg aggcggtgaa
     1801 gttcttccat ctcaacgccc tgtcctctcc gatcctcatc aaagtccact tcgatgtaat
     1861 catgaccatg atcgccgaca ctctctacgc tcggctgtcg caaaaccttc ggggcttcga
     1921 ggcctgcgat gctcccaagc tgtatcgcga ctttgtcaag ggcaaggggg aggtgctgat
     1981 caaggacggc cgtgtgctcg tcaagtatcc ccggcgggca cacaatccca ttttgcgggc
     2041 cgtcccccgg cagcggctgc ctcagaagat cccctggctc gacgatgttc caatcagctt
     2101 cgattttgcc tgacatgggc cacaaatg
//
LOCUS       DAOOVC010000309         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_28709_length_2123_cov_4.091393, whole genome shotgun sequence.
ACCESSION   DAOOVC010000309 DAOOVC010000000
VERSION     DAOOVC010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28709_length_2123_cov_4.091393"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLM79_18805"
     CDS             <1..213
                     /locus_tag="PLM79_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88411.1"
                     /translation="PGIILAAALFMYAVGFFCKEGTGAAGVAPWLLAVFSGMVALAAY
                     FSFLWLVGKDEWKSTVGLMAASWRRR"
     gene            210..902
                     /locus_tag="PLM79_18810"
     CDS             210..902
                     /locus_tag="PLM79_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88412.1"
                     /translation="MKKKTGLSPDAAPVLQGLCRTGKNRFAAVLFFWLTLLSGTGPLC
                     HAQDLLWGTEHTSRHYIIVISGQVNKEKFHGAKGLLTLSPPSPLSLNAYLLIIEGFPK
                     RNSRNSFFWNSEDSFMNAFSNEIVCDLKRAYAKFPDIHFFYLSPALLKSKGMYTHREK
                     ERKQLAEKTALPTKVFAASGRLRVTINADEVSGTVMMKGYDPIERGYAEYVAGFHGRM
                     TVPLESSKELKK"
     gene            1456..>2123
                     /locus_tag="PLM79_18815"
     CDS             1456..>2123
                     /locus_tag="PLM79_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88413.1"
                     /translation="MTPCGYGGGGRFTCIALHPHNPRVLMVGSDVAGVFRSDNGGREF
                     FVKGTGLEGFTAADIAFHPTREGFVALITADGLYVSADLGERWRKITPRIRYDQRFSG
                     SRLLVFREEVLWAATDREGVFRVEFGPVGPSIRSVAGLEGRRIHCLAQTSDALWAATD
                     RGLFRLDSGGWEARNSGLKASGIEVADLAAQPGGQLYVLEKSGGIYAWNEPKKEWAPR
                     RPPLP"
BASE COUNT          391 a          637 c          680 g          415 t
ORIGIN      
        1 cccgggatca ttctggccgc cgcccttttc atgtacgccg tgggattctt ctgtaaggag
       61 ggtaccgggg ccgccggggt cgccccttgg cttttggccg tgttttccgg tatggtggct
      121 ttggcggcgt acttttcttt cctatggttg gtggggaagg acgagtggaa atccaccgtc
      181 ggattgatgg ccgccagttg gaggcgaaga tgaaaaagaa gacgggcctt agcccggatg
      241 cagcccccgt tctacagggt ttgtgccgga ccgggaagaa tcgctttgcg gccgtcctgt
      301 tcttctggct gacccttctt tcgggcaccg gacccctttg ccacgcccag gacctcctgt
      361 ggggaaccga acataccagc aggcactaca tcatcgtgat ctccgggcag gtgaacaaag
      421 agaagttcca cggtgccaag gggcttttaa ccctctcccc gccttctccc ctgagcctca
      481 acgcctacct gctgatcatc gagggctttc ctaagagaaa ctcccgcaac agctttttct
      541 ggaattccga ggacagcttc atgaacgcct tttccaacga gatcgtgtgc gatctcaaac
      601 gggcgtacgc caagtttccc gacattcatt tcttctacct gagtcccgcg ctgctgaaat
      661 ccaaaggcat gtacacccac cgggagaaag aacgaaagca actggcggaa aagacggccc
      721 tgcccaccaa ggtctttgcc gccagcggcc gtttgagggt gacgatcaac gccgacgaag
      781 tttccggaac ggtcatgatg aagggatacg accccatcga gcgggggtac gcggaatacg
      841 tggcggggtt tcatggaaga atgacggtgc ccctcgagtc cagcaaggaa ttgaagaagt
      901 agacgggcac ggggcccgag ggaaaagggg aggcgacggt tttgggagcg cctcgaatcc
      961 tgcgcggcac acaatgaggg acccgacatg ggtaggaaac ggatcggtcc tccggccgaa
     1021 gtcggaatcc gcatagattg cctcgatccc gggctcggcg ggcctgccgg tccgtcggat
     1081 gatcgcggct gcgatcctgc acggcgtcca tcccccgggc cgggcgcgtt gcccggcgcg
     1141 aattttgtct cccccttccg acgaaagtca ggctttcact caacgggacg caacccgttg
     1201 caggaacgat tcccgcccaa gggccaatcc gcaaagaccg tgcaaacccc ctggatgccc
     1261 gccttggccc gcatgacgga ccgattctct cgggatcgct ccccaaacgc atccataagg
     1321 cacttgcgtc caagaccggg agggacggtt gggctcctct tcctgctgct cctttcgatc
     1381 cacgcatcgg ctttccccgt tcccccggtg caggccgggg aaattgccgc cccgggcacc
     1441 gggtcgggat ttgtcgtgac cccttgcgga tacggagggg gcggccggtt cacctgcatc
     1501 gccctccacc cgcacaatcc ccgggtgttg atggtggggt ccgatgtggc cggggtattt
     1561 cgcagcgaca acgggggccg ggaattcttt gtgaagggga ccggcctcga gggctttacc
     1621 gcggccgata tcgcttttca tccgacccgg gaaggtttcg ttgccctgat cacggcggac
     1681 ggcctctacg tgagcgccga cctgggggaa aggtggcgga aaatcacccc gcggatccgc
     1741 tatgatcaaa ggttttccgg aagtcgcctc ctggtgtttc gggaggaggt cttgtgggcg
     1801 gccacggacc gggagggggt ctttcgagtg gaattcggtc cggtgggacc ttccatccgg
     1861 tcggttgcgg gactggaagg gaggcggatt cactgcctgg cgcaaacctc ggatgccctg
     1921 tgggccgcca cggatcgagg gttgttccgc ctggattccg ggggctggga agcacggaat
     1981 tcgggtttga aggcctcggg gatcgaggtc gcggacctgg cggcccagcc cgggggacaa
     2041 ctgtacgtcc tggagaagtc cggaggcatc tatgcctgga acgagcccaa gaaagaatgg
     2101 gcaccccggc gccctcccct acc
//
LOCUS       DAOOVC010000310         2021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_30819_length_2021_cov_4.196846, whole genome shotgun sequence.
ACCESSION   DAOOVC010000310 DAOOVC010000000
VERSION     DAOOVC010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30819_length_2021_cov_4.196846"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLM79_18820"
     CDS             complement(<1..742)
                     /locus_tag="PLM79_18820"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOV88414.1"
                     /translation="MPIVTLLSDFGTRDGYVAAMKGVILGICPGACLVDVTHEVPPQD
                     VRAGAFVLSTVYEHFPPGSIHVAVVDPGVGTDRKALGLQAARQFFVGPDNGIFSLVLR
                     KHPDRTAYSLENPAFRKEPVSRTFHGRDLFAPAAAHLAAGAPLESMGPPCTPGMEPWS
                     SPRSSPREILGEAIYVDHFGNIITNITRDDLGSAASLPSWRIRVGRTPVPFSGDTYGR
                     AAPGTLLAYWGSAGFLEIAVNRGNAALTL"
     gene            907..1707
                     /locus_tag="PLM79_18825"
     CDS             907..1707
                     /locus_tag="PLM79_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOV88415.1"
                     /translation="MVQLEKPAVEDLTKFAVEMVRTVGDEALAYYGKGNPEVKFDEEL
                     VTAAELHLVELFRGKLHERFPQHGLFGDDLPSKDYVHGQEGYLWILDALDGVANFQAG
                     VPIWGTSLALLENFWPILGVFYMPVTGDLYSARAGQKAFWGQEPIRIPNVGDLSNESL
                     LLTYSRFHNHYHSVFPGKIRNLGSTAAHICYVARGRAEAALLAHVSYQDLAAAQIILA
                     AAGGEIRKLDGSAFHLSEYLGGSRIEEHLLAAPKGAHSSIAMYLEELG"
     gene            complement(1742..1957)
                     /locus_tag="PLM79_18830"
     CDS             complement(1742..1957)
                     /locus_tag="PLM79_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88416.1"
                     /translation="MVPILPTDREIAARQGQVPGWEQSWMRTLRKAEAVRALGAKLPP
                     ELLLQLEETLNRTERELDTVLSEAGKG"
BASE COUNT          366 a          625 c          645 g          385 t
ORIGIN      
        1 ccagcgttag ggcggcgttt ccccggttga cggcgatttc gaggaaaccg gcgcttcccc
       61 agtatgccag gagggttccg ggtgccgccc gaccgtaggt gtcccccgag aaggggacgg
      121 gggtcctgcc cacccgaatc cgccatgaag gcagggaagc ggcggagccc aggtcgtccc
      181 gggtaatgtt ggtgatgatg tttccaaagt gatccacgta gattgcttcc ccgaggatct
      241 cccgggggga ggaacggggg gagctccagg gttccatccc gggggtacag ggggggccca
      301 tggattcgag gggggcgccc gcggccagat gcgccgccgc cggggcgaaa aggtctcgac
      361 cgtggaaagt ccggctcacc ggttccttcc ggaaggcagg gttctcgagg ctgtaggcgg
      421 tccggtcggg gtgcttcctc agcaccaggg aaaagatgcc gttgtccggc cccacgaaga
      481 attgtcgggc cgcttgaagc cccagggctt ttcggtcggt accgacaccc gggtccacca
      541 cggcaacgtg aatgcttccc ggaggaaaat gttcatacac cgtggacagg acaaaggccc
      601 cggcccgcac gtcctggggg gggacctcgt gggtgacgtc cacgaggcag gctccgggac
      661 agatgcccag gattacccct ttcatggcgg ccacatatcc gtcccgggtg ccgaaatcgc
      721 tgagcagagt gacaatgggc atcgggttgt ttcctccctt ttcggcttgc tatctcttcg
      781 agctttgtta acatgaaatt cttcgatgta aagaaagact tggatgcccg gaggagcctg
      841 cgggcagttt caggaatccg gaacacgatc agggagctac gagtcatttc ttctggggga
      901 gcaaacatgg tgcaactgga gaaacctgcg gtcgaggatt tgacgaaatt tgcagtggaa
      961 atggtgcgga ccgtggggga tgaagccctg gcctactatg ggaaaggaaa tcccgaggta
     1021 aagtttgatg aggaactggt caccgcggcg gaattgcacc tggtggagct ttttcgcggc
     1081 aagctgcacg agcgatttcc ccaacacgga ttgttcgggg acgatcttcc ttccaaggac
     1141 tacgtccacg ggcaggaagg atacctctgg attctggacg ccctggacgg cgtagccaat
     1201 tttcaggcgg gggtccccat ctggggcacg tccctggctc tcctggaaaa cttctggccc
     1261 attctcgggg ttttctatat gccggtgacg ggggaccttt acagcgcccg ggcggggcag
     1321 aaagcgttct gggggcagga gcccatccgg attcccaacg tgggggacct gagcaacgag
     1381 agcctgctcc tcacgtactc gaggttccac aaccactatc attccgtgtt tccgggaaaa
     1441 atccgcaacc tggggtccac ggcggcccac atctgctatg tggcccgggg cagggccgaa
     1501 gcggccctcc tggcccatgt gtcctatcag gatctggcgg cggctcagat catcctcgcg
     1561 gcagcaggcg gagaaattcg caagctggac ggatcggcct tccacctgag cgaatacctg
     1621 ggaggatcga gaatcgaaga gcacctgctt gcggccccca aaggagcaca ctcctccatc
     1681 gccatgtacc tggaggaatt gggctgaaag attgcggcgt tggccgaggg tctttggggg
     1741 gctacccctt cccggcttcg ctcagcaccg tatccagttc cctctcggtg cggttgagcg
     1801 tctcttccag ttgcaaaagc agttccgggg ggagttttgc ccccagggcc ctgacggctt
     1861 cggctttccg cagggttctc atccagcttt gctcccaccc gggcacctgc ccttgccggg
     1921 cggcgatttc ccggtcggtg gggagaatcg ggaccacttc ccccctcccg cccccccccc
     1981 cccccccccc cccccccccc cccccccccc cccccccccc c
//
LOCUS       DAOOVC010000311         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_31389_length_1997_cov_6.488671, whole genome shotgun sequence.
ACCESSION   DAOOVC010000311 DAOOVC010000000
VERSION     DAOOVC010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31389_length_1997_cov_6.488671"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(555..1301)
                     /locus_tag="PLM79_18835"
     CDS             complement(555..1301)
                     /locus_tag="PLM79_18835"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOV88417.1"
                     /translation="MKKRLSEHLARLGCRRVEDYLRLLHEVPSSRREALLLMGVTISR
                     FFRDLRVWDALESRVIPEILASGLREVSVWSAGCGRGEEVYTLKMLWEHLRENRPHLP
                     DLTIRATDRNPENLAAAAMGIYPKSTLREVPALFRERFFSELGKGRVAIVASSLHENI
                     LWMVHDLMEDPPPRGGFHLVFLRNSLLTYYGESRAARALPRVLSSLGDGGFLVIGRKE
                     SLPSSVESLRGFLPEEGIYRKAWSRGGRTL"
     gene            complement(1426..1815)
                     /locus_tag="PLM79_18840"
     CDS             complement(1426..1815)
                     /locus_tag="PLM79_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOV88418.1"
                     /translation="MSCRSIETNRAPAAIGPYSQAVSVGEWVFLSGQIPLVPETGELV
                     AGDFGEQARQVLKNLGQVLAAAGCSWKDVVSVDVFLTDLGRFAEFNGIYSEHLEGHKP
                     ARAVVEVKGLPRGALIEMKCVAHQHRA"
BASE COUNT          378 a          687 c          563 g          369 t
ORIGIN      
        1 cctcctcccc cggcaggtac aggcaaaaac ccgccgggag catttccacc ccgactccaa
       61 ggacctccgc cgccccggcg gccaattcct ccgcccacgg atccgggcag gaaacctcca
      121 cgcgaaccca ccggccttcg gttccggaaa ccatgcacac tcactttccc ggcatccggc
      181 aaggacctcc catgggaatg tcggagagag tccgccggat gaaactcgtc tgacgcccta
      241 ttcacaaaca ggggatccgt tctcccccgc ctccccccac cccgggaggg aagaccgccc
      301 ccccggcaaa cgacggtcgg ctgttgggcg cggggagagc tcacatctcc cgaacaaccc
      361 caaatcgccg tcctgcaggc gcaaacccat ctgtgccgac tcgaggcctt ttatcacgag
      421 gacgcgaagg acgaaagccc ccggaaggtc tccgtgttcc gggcggtcgg cctcttttca
      481 agggcccggg cgaaactccg agcgcaatcc tcggtgggat tcccacgggc tttcgccggg
      541 aatcgtcctg ctgcttacag ggtccgccct cccctcgacc aggcctttcg atagattccc
      601 tcctcgggga gaaacccccg gagactttcc accgacgacg ggagagactc cttgcggccg
      661 atcaccagga atcccccgtc ccccaggctc gagaggaccc tcggcagggc tcgggccgcc
      721 ctcgattccc cgtagtaggt gaggaggctg tttcgaagaa ataccaggtg aaaacctccc
      781 cggggaggcg ggtcttccat gagatcgtgc accatccaca ggatgttttc gtggagggag
      841 gaagcaacga tcgccaccct acccttcccc agctccgaga agaatctttc tcggaaaagg
      901 gcggggacct ccctcagggt gctcttgggg tagatcccca tggccgccgc ggccaggttc
      961 tcggggtttc gatccgtagc ccggatcgtg aggtccggga ggtgcggccg gttttcccgc
     1021 aggtgttccc acaacatctt cagggtgtac acctcttccc cgcggccgca ccccgccgac
     1081 cacaccgaca cctctcgaag ccccgaagcg aggatctccg gaatgacccg ggactcgagg
     1141 gcgtcccaca cccggagatc ccgaaaaaac cggctgatgg tgacccccat gaggagcagg
     1201 gcttcccggc ggctggacgg gacttcgtgg aggaggcgca ggtagtcttc cacccgccga
     1261 cagcccaacc gggccaagtg ttcggagagg cgcttcttca caccctttcg caccctccga
     1321 tacccttccc acgacaaccc caaccgccgg agaatttccc ggaactgatc gtcggtcata
     1381 gcctggaggc cggttttggg aagatcgaat tcatttggtc tccactcatg cccggtgttg
     1441 atgggccaca catttcattt caatgagggc tccccggggc aggcccttga cttccaccac
     1501 ggctcgggcg ggtttgtggc cttccaagtg ttccgaataa atcccgttga actcggcaaa
     1561 ccggcccaag tccgtgagga aaacgtccac actcaccacg tctttccaag aacacccggc
     1621 ggcggcgagg acctgcccga ggtttttcag cacctgccgg gcctgctccc caaaatctcc
     1681 ggccacgagt tctcccgtct cggggaccag cgggatctgg ccgctcaaaa atacccactc
     1741 ccccacgctc actgcctggg aatagggacc gatggccgcg ggggcccggt ttgtctcaat
     1801 gctccgacag ctcatgttca cctcctggga tgacctggtt acggttgatc gctttgggcg
     1861 gaaagaagcc tcgaattcct tgactcccgg cctgcccgaa ggagaaacgg ttctttggcg
     1921 actcgtcgtg tttgcgactc ggatttgctc ttgacttggg gcaagagttt tgtctaggat
     1981 cgttttcatg tcgattg
//
LOCUS       DAOOVC010000312         1985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_31680_length_1985_cov_3.876166, whole genome shotgun sequence.
ACCESSION   DAOOVC010000312 DAOOVC010000000
VERSION     DAOOVC010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31680_length_1985_cov_3.876166"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..712)
                     /locus_tag="PLM79_18845"
     CDS             complement(509..712)
                     /locus_tag="PLM79_18845"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOV88419.1"
                     /translation="MKQIIVDITNEGEMKLETRGFVGRGCLEEAQFLKDVLGEETARQ
                     LTPAYYQRQGQKEAVKKHLPLCG"
     gene            complement(709..942)
                     /locus_tag="PLM79_18850"
     CDS             complement(709..942)
                     /locus_tag="PLM79_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88420.1"
                     /translation="MPKETEFAEPQASETRANLIPIRQAYFPKAFWDFWDRLDDRQKA
                     AYILGWICELEMKGRRTSEQVQRINDHLEDDGK"
     gene            complement(992..1411)
                     /locus_tag="PLM79_18855"
     CDS             complement(992..1411)
                     /locus_tag="PLM79_18855"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HOV88421.1"
                     /translation="MSHVAKIELEINDLDTLKAACVRLGLEFIQDQKHYRWYNEHVGD
                     YPLPEGFTVDDLGRCDHAIRVPGASYEVGIVRRGSKYVLLWDFYSSGGLERKLGKDAG
                     RLKQAYAVERVHREAKLKGYHVQERKTEQGIRLVLTK"
     gene            complement(1414..1611)
                     /locus_tag="PLM79_18860"
     CDS             complement(1414..1611)
                     /locus_tag="PLM79_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88422.1"
                     /translation="MTFIVFDLTDFHVQQRDATTRVRVRFVQAKNMEKAKELMRTCYP
                     GAWAIIPKKTFDKGIVHAEER"
     gene            complement(1608..1820)
                     /locus_tag="PLM79_18865"
     CDS             complement(1608..1820)
                     /locus_tag="PLM79_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88423.1"
                     /translation="MKIIRKGVNFKSEERAIRNQVHQGLRCADCLKPFNGGERKHFKA
                     GSGAVICQKCCADESSADIPGEEAIR"
BASE COUNT          373 a          566 c          517 g          529 t
ORIGIN      
        1 aggacgtgct gagctgcagg agacatgact cctggaaaat atcgcttttc atggcggttc
       61 ctccctgggt gggtactttc tatcggggtt ctggttttga tcgctcaatc gcgatttcac
      121 cggggacttt tcgcgcagga tggcgcgtta gagccttggc cctagtgcgc atcttctctc
      181 cgtaattggg gttttttccg aaccggaaga ggtgcagagt gcaagactgc gtcgtgcatc
      241 cggaaacatc ctggaagctc cccccaacgc agtcgagaca cctctttcgg atcgccccaa
      301 gtggtgaggg gttctcgata tcgggcatct ttccagaacg atacggatta agcggacacc
      361 tggttgcagg gcagaggttt acttcctggg cctgattgtt ggcgcaccac aagcaatgcc
      421 ggcgtattgc tcttagcggc gtcatctgac ttccttcggg taaatcggtg attcggggaa
      481 tcgtaagagg cccgcggatt gattcatatc agccgcagag gggaaggtgt tttttcactg
      541 cttctttttg gccttggcgt tgatagtagg cgggggtgag ctgcctggct gtctcttcgc
      601 cgagaacatc cttgaggaat tgggcctcct caaggcaccc tcttccgaca aacccccgcg
      661 tctccagctt catttcacct tcgttcgtga tgtcgacaat gatctgcttc atttcccatc
      721 atcctccagg tgatcgttga ttctttggac ctgctcggag gtcctgcgac ccttcatctc
      781 cagctcgcaa atccatccga gaatgtatgc ggccttctgc cgatcgtcca accggtccca
      841 gaagtcccaa aacgcctttg ggaagtaagc ctgacggatc gggatcagat tcgcccgtgt
      901 ctccgaagct tgcggttctg caaattcggt ttcctttggc attgcctttt ctccgtatcc
      961 ataagttgca ggtatgctct catgatctcg ctcacttcgt cagaaccagg cgaataccct
     1021 gttcggtttt ccgctcttgg acatggtagc ccttgagctt cgcttcgcgg tggacccgct
     1081 cgactgcgta tgcctgcttg agcctgccgg cgtctttgcc aagctttcgc tcgaggcctc
     1141 cggacgagta gaaatcccag agaagcacat atttgctccc gcgtcggaca atgcccacct
     1201 cgtaggaggc gcccggcaca cggatggcat gatcgcaccg gccaaggtca tctaccgtga
     1261 atccttcagg aagagggtag tctcccacat gctcgttgta ccaccggtag tgcttctgat
     1321 cctggatgaa ctcaagccca aggcgcacac aggctgcctt gagggtgtcg agatcattga
     1381 tctccagttc tatttttgct acgtggctca tctctatcgc tcctccgcat ggacgatgcc
     1441 cttatcgaag gtcttcttcg ggatgatcgc ccaggcgccc gggtagcagg tgcgcataag
     1501 ctcctttgcc ttttccatgt tctttgcttg cacgaagcgc accctcaccc tggttgtcgc
     1561 atcacgttgc tgcacgtgga aatcggttag atcgaaaaca atgaacgtca acggattgcc
     1621 tcctctcctg ggatatcggc ggagctctcg tccgcacagc acttctggca gatgacggca
     1681 cctgatcccg ctttgaaatg cttccgttcc ccaccattga acggcttgag gcaatccgcg
     1741 catcgcaaac cctgatggac ctggtttcta attgcccttt cctcgctctt gaagttcacg
     1801 cctttgcgga taattttcat gatttcagct ccttttcagt tgaatctttt ggtcgaattc
     1861 ggagaaagat ccatgcaggt atccctggaa ctggcgcatg tgcattgtaa agagcgcctc
     1921 aaggagtctt gcgtcttcct cccggtcatt gaggcggtag tacatgatga gccttccgaa
     1981 tcctc
//
LOCUS       DAOOVC010000313         1966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_32148_length_1966_cov_6.525379, whole genome shotgun sequence.
ACCESSION   DAOOVC010000313 DAOOVC010000000
VERSION     DAOOVC010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32148_length_1966_cov_6.525379"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="PLM79_18870"
     CDS             complement(<1..1264)
                     /locus_tag="PLM79_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOV88424.1"
                     /translation="MLERPFVHSSLEEPGSSFPVRYPSELPITSKRQEILDALKNHQV
                     VVITGETGSGKSTQIPKMCLEAGLGASGWIGCTQPRRIAAVTLANRVAEELGGAGPAL
                     VGYKIRFRDRTRRSNRIKFMTDGILLAEAQTDRLFRSYGAIVVDEAHERSLNIDFLLG
                     MLKGVLPKRPDLRVVITSATINPEKFSQAFGSAPIVEVSGRTFPVQVIYRPVSAPDED
                     PEEVTHIDQTLASIQEIQSITRPGDDGDTLVFMPTESDIRETVQRLEELRLRNTLVMP
                     LFGRMAAPDQRKIFESTPQHKIIVATNIAETSITIPGVRYVVDTGLARISQYNAGSGT
                     QGLPVVPVSRASADQRKGRCGRLGPGICFRLYSEEDYLSRPPYTLPEIQRANLAEVIL
                     RMLSLRLGDIREFPFLDPPSPGAVRDAFS"
     gene            1652..>1966
                     /locus_tag="PLM79_18875"
     CDS             1652..>1966
                     /locus_tag="PLM79_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOV88425.1"
                     /translation="MSSIRDQIGEAAAIIGRHLKATPEIGMILGTGLGSVAEEIEEPA
                     TVPYSAIPHFPVSTVQGHQGQILGGKWTGKSILAMQGRFHLYEGYTPQQIAFPVRVMK
                     ALG"
BASE COUNT          405 a          581 c          617 g          363 t
ORIGIN      
        1 cggagaatgc gtcccgaacc gctccggggg agggcggatc caggaacggg aattcccgga
       61 tgtcccccaa acgaagggaa agcatccgga gaatgacttc ggcaaggttg gctctctgga
      121 tttcgggcaa ggtgtaaggc ggacgcgata ggtaatcctc ttccgagtac aggcgaaaac
      181 agatccccgg gcccaatctg ccgcagcggc ctttgcgctg gtccgcactg gcccgggaaa
      241 cgggaaccac cgggaggccc tgagtcccgg atccggcatt gtactgagaa atgcgcgcca
      301 gcccggtatc caccacgtac cggaccccgg gaatcgtgat ggaggtctcg gcgatgttgg
      361 tggcgacaat gatcttgtgc tggggggtgg actcgaagat cttccgctga tccggggccg
      421 ccatgcgacc gaagaggggc atgaccagag tgttccgaag tctcagctct tccagtcgct
      481 gaaccgtttc ccggatgtcg ctttccgtgg gcatgaagac gagggtgtcc ccgtcatcgc
      541 cgggacgggt gatggattgg atttcctgga tggacgccag ggtctggtcg atgtgggtga
      601 cttcctccgg gtcctcgtcc ggggcggaga cgggacgata gatcacttga accggaaagg
      661 tccgtccgct gacttccact atgggcgccg agccaaaggc ctgagagaat ttttcgggat
      721 tgatggtggc cgatgtgatc acgactcgca ggtccggtcg tttgggcaga acccccttga
      781 gcatccccag aaggaagtca atgttcaggg aacgctcgtg agcctcgtcc acgacgatgg
      841 ctccgtaaga gcggaaaagg cgatcggtct gggcttccgc cagcaggatg ccgtccgtca
      901 tgaacttgat gcggttggaa cgccgggtgc ggtcccgaaa acgaatcttg taaccgacca
      961 gggcaggacc cgcgccgccc agttcttcag ccacccggtt ggccagggtg acggcggcaa
     1021 tccttctggg ctgggtgcac ccaatccacc cggacgctcc cagaccggcc tcgaggcaca
     1081 ttttcggaat ctgggtactc ttgcccgatc cggtctcccc ggtaatgacc accacctggt
     1141 ggttcttgag ggcatcgaga atctcctgcc gtttggacgt gatgggaagc tcggaagggt
     1201 agcgaacggg gaaggaactt cccggttctt ccaaggaaga atgaacgaag ggtctttcaa
     1261 gcataaaatc ccgcacccgc caacgagcgt gagagccccg gcttcaaaag gcccctcggc
     1321 accccccgcc gcacccacgg ggcatccgac gaacccttcc gcgtcctttc gtgcacaacc
     1381 cgggcaaaac tctcgggaac ccctcgatct cggcggggat gacgcttcaa cctcgactcg
     1441 aaacagccca ttttcgacct cgttcgaaag attgccggag cacagtgctc aaatcggtgc
     1501 aaagaaagcc ctaccgagaa atctttacaa atctcccggg ttgtgtcaat tgtctgaacc
     1561 gatttgtggg gtgcccgacg ggccggaaag catttgcttg agaggccccg ccgaggtttt
     1621 gtgcccccat gcgaccggac gaggtgaaaa gatgagctcg attcgcgacc aaatcggtga
     1681 ggcggccgcc atcatcggca ggcacctgaa ggcgaccccg gagatcggca tgatcctcgg
     1741 gacagggttg gggagcgttg cggaagaaat cgaggaaccg gcgaccgttc cctactccgc
     1801 catcccgcat tttcccgtgt ctacggtaca gggccaccag gggcagatcc tgggcgggaa
     1861 atggacgggc aaatccatcc tggccatgca gggccgtttt cacctctacg aagggtacac
     1921 gccgcaacag atcgccttcc ccgtccgtgt gatgaaagcc ctgggg
//
LOCUS       DAOOVC010000314         1855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_35063_length_1855_cov_5.491667, whole genome shotgun sequence.
ACCESSION   DAOOVC010000314 DAOOVC010000000
VERSION     DAOOVC010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35063_length_1855_cov_5.491667"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1855)
                     /locus_tag="PLM79_18880"
     CDS             complement(<1..>1855)
                     /locus_tag="PLM79_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOV88426.1"
                     /translation="RQLANPRLYLMILADCVLFAVSLVGAFLLRFEFVLTPEQVKEIG
                     LILIYLIPCKLIIFHIFGLYRGMWRYTSVGDFWRLTQACGAATLLLLAILLYTYRFVG
                     FSRAVFLVDGALTLLLTGGLRVTIRSYFTIKGGSMGIQAFPMWTGVQKGDKRILIIGA
                     GGSGEKLLREVFDNPQLSYQVVGFLDDNSGKHGRTVHGIPVLGPTQRLGEVVREYSVE
                     QVFISIPSATGAEMRRILETCKGCGVSYKTLPAIGEIMKGKVSIKALRDVNYEDLLRR
                     PPVELDTTGIEEYLADRTVMVTGGGGSIGSELCRQLLRFRPSRLILVDAGETNLYNIQ
                     MELEHELRFRHYVCILGRVQNRSLMDEVFEKYRPEIIFHAAACKHVPMLEKNPWEAVF
                     NNVMGSQVVMELAKKHGTRRFVFVSTDKAVRPTNVMGTSKRLAELILQSMENETTRYM
                     AVRFGNVVGSSGSVIPLFRNQIRQGGPVTVTHPDITRYFMTIPEACQLILQAGALGRG
                     GEIFVLEMGTPVKIADMARDLIRLSGKEPGRDIEIVFTGLRPGEKLHEELITDGEDVT
                     RTRHEKIMVLKCNGKGPLNGTQYLQVPAEPLSGELEQLYRAALANDAESIKT"
BASE COUNT          396 a          556 c          525 g          378 t
ORIGIN      
        1 gtcttgatgg attccgcgtc gtttgccagg gctgcacggt aaagctgctc cagctcgccc
       61 gaaagaggtt ccgccgggac ctggagatat tgggtgccgt tcaaaggccc cttgccgttg
      121 cacttgagga ccatgatctt ctcatgccgg gtacgggtta cgtcttcccc gtcggtaatg
      181 agttcctcgt gaagcttctc ccccgggcgc aggcccgtaa agacgatctc gatatcgcgc
      241 cccggttctt tgcccgaaag tcgaatgagg tcccgggcca tgtcggcgat ctttaccggg
      301 gtgcccatct ccagcacaaa aatctccccg ccccggccca gggccccggc ctggaggatc
      361 agctgacaag cttcgggtat ggtcatgaaa taacgggtga tatccgggtg agtcacggtc
      421 accggccccc cctgccggat ctggtttcga aacagcggga tcaccgaccc cgatgatccc
      481 accacgtttc caaacctcac cgccatgtac cgtgtcgtct cgttttccat ggactggaga
      541 atcaactcgg ccaatcgctt gctggttccc atgacgttgg tgggccgcac ggctttatcc
      601 gtggatacga aaacaaaacg ccgggtcccg tgcttcttcg ccaattccat cacaacctgg
      661 ctgcccatca cgttgttgaa aacggcttcc cacgggtttt tctccagcat gggaacgtgc
      721 ttgcaggccg cagcgtgaaa gatgatctcc ggccggtatt tctcaaagac ctcatccatg
      781 agggagcggt tctgaacccg ccccaggatg catacgtagt ggcgaaaccg gagttcgtgt
      841 tccagttcca tctgaatatt gtaaagattc gtttctcccg catccaccaa gatgagccgc
      901 gagggtcgga agcgaaggag ctggcggcag agttcggacc cgatggatcc gccccctccc
      961 gtcaccatga ccgtccggtc tgcaaggtac tcttcgatcc cggtcgtatc caactcgacg
     1021 ggaggtcttc gcaaaaggtc ttcgtaattc acgtcccgca aggccttgat gctgaccttg
     1081 cccttcatga tctccccgat ggcaggcaga gtcttgtagg aaacgccgca tcccttgcac
     1141 gtttcgagaa ttcggcgcat ttccgcaccc gtagccgaag gaattgagat aaacacctgc
     1201 tccacagagt actcgcgaac aacttctccg agtctttggg tgggaccgag gaccggaatg
     1261 ccgtgaacgg tgcgcccgtg ctttccggag ttgtcatcca ggaaccccac cacctgatag
     1321 ctcaattggg ggttgtcaaa aacctcgcga agcagtttct cccccgatcc gccggctccg
     1381 atgatcagga tccgcttgtc tcccttctgt accccagtcc acatgggaaa ggcctggatc
     1441 cccatcgagc cgcccttaat ggtgaagtag gaccggatgg tcacgcgaag accgccggtc
     1501 aggagtaagg tcagagctcc gtccaccagg aaaaccgcgc gggaaaaacc cacgaatcgg
     1561 taagtgtaga gcaggatggc gaggagcagt aaggtggccg ctccgcaggc ctgggtcaga
     1621 cgccagaagt cccccacgct cgtgtagcgc cacatgcccc ggtaaaggcc gaagatatgg
     1681 aatatgatca gcttgcaggg gatgaggtag attagaatca ggccgatttc cttcacctgc
     1741 tcaggggtga ggacgaattc gaagcgcagg agaaacgccc ccaccagcga cacggcgaaa
     1801 aggacgcagt cggcgagaat cattaagtag aggcgggggt tggcaagttg gcgca
//
LOCUS       DAOOVC010000315         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_36281_length_1814_cov_11.120523, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000315 DAOOVC010000000
VERSION     DAOOVC010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36281_length_1814_cov_11.120523"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PLM79_18885"
     CDS             <1..385
                     /locus_tag="PLM79_18885"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOV88427.1"
                     /translation="CNHCDNPPCVYNCPTGASYKSKDGTVQVDKKRCVGCKACIAACP
                     YDARYITPEGIADKCTFCEHRVREGKLPACVATCIGSSRIFGNLDDPNSTVSRILRET
                     SASVLLEKAGTNPRVFYVRKLGKAE"
     gene            395..1363
                     /gene="nrfD"
                     /locus_tag="PLM79_18890"
     CDS             395..1363
                     /gene="nrfD"
                     /locus_tag="PLM79_18890"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOV88428.1"
                     /translation="MEITITGMNAITHPHLDIWNWMVATYLFLGGVAAGLLIMSAVAN
                     LRNVGSEGKDHPSCVRAALLAPFVLMVGMFFIFLDLENKLHSFWFFLSFKPASPMSWG
                     SWGLALIVPISMLYGLSVITEDHKSWLRFEFLKRLSDRLNPYVRPLAMINFGLGIFLG
                     IYTGILLSVFVARPLWNSSLLPILFLASAASTGAALTVLIAGKKSAKLYFTKIDIGLI
                     ALEIVLILLFFAGHITSTEPHRESIMPFFSFTSEYFMFVAAWILLALLFPMALVLALL
                     EVRESEGEELSSSALLRMKLSAVLVLTGAFIVRIALVYAGQLSKLT"
BASE COUNT          393 a          469 c          463 g          489 t
ORIGIN      
        1 ttgcaaccac tgcgacaatc ctccttgtgt gtacaattgt cccaccggag cttcctacaa
       61 gagcaaggat ggaactgttc aggtcgataa aaaacgctgt gtcgggtgta aagcctgtat
      121 tgccgcctgc ccttatgatg cacgctatat aaccccggaa ggaatcgcgg ataagtgcac
      181 attttgcgag catcgagtcc gggaaggcaa gctgccggcg tgtgtggcaa cctgtatcgg
      241 gtcctccagg atctttggaa acctggatga tcccaacagc acagtgagcc gcattttgag
      301 ggaaacgagc gcctccgttc ttctggaaaa ggcgggaacc aatccaaggg ttttttatgt
      361 cagaaaattg gggaaagctg aataggagat cactatggaa atcactataa ccgggatgaa
      421 tgccatcacg catccccacc tcgacatctg gaactggatg gttgcaacct acctcttcct
      481 gggcggtgtt gccgcaggac tgctcatcat gtcggcagtg gcaaacctca gaaacgtcgg
      541 ttccgaggga aaggaccacc catcctgcgt tcgagcggct cttttggctc catttgtcct
      601 gatggtcggc atgttcttca tctttttgga ccttgaaaac aagctccact cattctggtt
      661 cttcctgtca tttaaacccg cttctccgat gtcttggggg tcctggggac tcgccctgat
      721 cgtccccatc agcatgctct acgggttgtc cgtgatcacg gaggatcaca agagctggtt
      781 gaggtttgaa tttctcaaaa gactctcgga caggctgaat ccgtatgtgc gtcctttggc
      841 gatgatcaac tttgggctgg ggatttttct cggaatttat acgggaatac tcctgagtgt
      901 ctttgttgca aggcctcttt ggaattcttc ccttctgccc attctattcc ttgcatccgc
      961 agcttccacc ggagcggccc tcacggtgct catcgcaggg aagaaatccg ccaagctgta
     1021 tttcacaaaa atcgatattg gcttgattgc gctagaaatt gtcctcatcc tacttttttt
     1081 tgccggccat atcacatcaa cagagccaca tcgggaatcg atcatgccct tcttttcgtt
     1141 caccagtgaa tatttcatgt tcgttgcagc atggattctt ctggccctgt tgttcccgat
     1201 ggcccttgtt ttggcacttc tcgaggttcg agagagtgag ggggaggaat tatcctcttc
     1261 cgcgttgttg agaatgaagc tgagcgcggt attggtcctt acgggtgctt tcatcgtaag
     1321 aatcgccttg gtctatgcgg gacaactgag caaattgact tgatagcact ctggaactgg
     1381 taatccgtca cggccacaag attaccagtt ccacatcacc aaacagcaag aaatctttcc
     1441 gatattcctt ggttttctgc ccagactctc cctgttgccg gaggagagat cttgcgatgt
     1501 ttcccctacc agcgcataaa tctgcatatc ccctcttttg aaaactattc gctggggtgc
     1561 tttttgcatt gtctccttac gtttcacatc tcggtgatat cgaggggcaa agaggttgga
     1621 ggcacctcgt agttttcacc gggaacccgg cgagatccag aggagagctt gaatgagtcc
     1681 caatgtccgc attgtcggcc gccaaatgcc gttctccgcc aaagaaggtg aaacccttct
     1741 cgacgtcctt ctagacgccg ggttttcggt ttccgccgat tgcgggggaa aggggaggtg
     1801 cggccggtgc aagg
//
LOCUS       DAOOVC010000316         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_38616_length_1742_cov_5.472436, whole genome shotgun sequence.
ACCESSION   DAOOVC010000316 DAOOVC010000000
VERSION     DAOOVC010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38616_length_1742_cov_5.472436"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="PLM79_18895"
     CDS             <1..1450
                     /locus_tag="PLM79_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOV88429.1"
                     /translation="STCIIGRARDCNLRLPNDAAHRVVSRHRCLLDINPPEIRIRDFG
                     SLNGTFVNGEKIGQRHSDESPDEGALRDYPEHDLVEGDLIGLGQTVFRVDVRVPAICA
                     GCFEEIPESRDVQPMKNGFHFCDACLAKSVEARNPPLPQRPRCALCGKEIFEDPHHGR
                     PGQVVCPACRLDPLEQIKGLLLAAAGGNKNLVAVEGFRILKELGRGGMGRVYLARHEA
                     TNREVALKVMLPQVAADERAREMFERECANHRALRHPHVVQVFDHGCSNGVFYLTMEY
                     CPGGSLDRRLGSAGGKLSLDSVLELALPALDGLDYIHNAEIPFVKLKDGTYGPGRGLV
                     HRDLKPGNLFLSSSGPDARIRIADVGLAKAFDLAGLSGLTVTGQAAGTPEYMPRQQVL
                     QFKYSRPEVDVWAMAATFYHLLTGCLPRDFSPGKDRWLTVLQTRPVPIRRRDPALPRR
                     FAAAMDHALTDDPEIGFKTAAEFKRALEESLS"
     gene            1447..>1742
                     /locus_tag="PLM79_18900"
     CDS             1447..>1742
                     /locus_tag="PLM79_18900"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOV88430.1"
                     /translation="MTKLSFSGLTDVGRVRRRNEDNWAAHPDRGIFLVADGMGGAARG
                     DLASRIVADTLPTLLPQSLEDLSEPRIKAMAQAAGEALIQLNGRVTREADSNPEL"
BASE COUNT          327 a          595 c          527 g          293 t
ORIGIN      
        1 ctccacctgc atcatcgggc gcgcccggga ctgcaacctg cggctgccca acgatgccgc
       61 ccatcgcgta gtttcccgac accgctgcct tttggacatc aacccccccg aaatccgtat
      121 tcgcgatttc ggaagcctca acggcacttt cgtgaacggc gagaagatcg ggcaaaggca
      181 ctcggacgag tcccccgacg aaggggcttt gagagactac ccggaacacg acctggttga
      241 gggggacctc atcggcctgg gacaaacggt tttccgagtg gacgtgcggg ttcccgccat
      301 ctgcgccggg tgttttgaag aaatccccga gtcccgggac gtccagccca tgaagaacgg
      361 cttccacttc tgcgatgcgt gcctggccaa gagcgtcgag gcccggaacc cacccctgcc
      421 ccaaagaccc cgatgcgctc tttgcggaaa agagatcttc gaagaccccc accacggaag
      481 gcccggccag gtggtctgcc cggcctgtcg cctggatccc ctcgaacaga tcaaggggct
      541 gctgctggct gcggcggggg gaaacaagaa tcttgtggct gtcgaggggt tccgcatcct
      601 caaggaactg ggccgaggag gcatgggaag agtctacctg gcccggcacg aggcaaccaa
      661 ccgggaagta gccctcaagg tcatgctgcc ccaggtggcg gcggacgagc gcgcccggga
      721 gatgttcgag cgggaatgcg ccaatcatcg agcccttcgt caccctcacg tggtgcaggt
      781 tttcgaccac ggctgttcca acggtgtctt ctacctaacc atggaatact gccccggagg
      841 cagcctggac cggaggctcg gatccgctgg aggaaagctc tccctggact cggtcctgga
      901 gctcgctctc cccgccctgg acggcctcga ttacatccac aatgccgaaa ttccctttgt
      961 gaaactgaaa gacggaacct acggcccggg ccgcggcctc gtgcaccggg acctcaaacc
     1021 cgggaatctc tttctcagtt cttccggccc cgatgcccgg atccggatcg ccgacgtagg
     1081 tttggccaag gccttcgacc tggcgggcct cagcgggctt accgtgacgg gacaggcggc
     1141 gggaaccccc gagtacatgc cccggcagca ggtgctccag ttcaagtact cccggcccga
     1201 agtggatgtc tgggccatgg ccgccacctt ctaccacctg cttacgggat gccttccccg
     1261 ggacttctcc ccgggcaaag accgctggct gacggttctc caaacccgcc cggttcccat
     1321 tcgccggagg gacccggccc ttccccgcag gttcgccgca gcaatggatc atgccctgac
     1381 ggacgaccct gaaatcggtt tcaagacggc cgcggaattc aaacgggcct tggaggaatc
     1441 cctgtcatga cgaagctctc cttttccggc cttacggacg tgggacgggt ccggcgacga
     1501 aacgaagaca actgggccgc ccaccccgac aggggaatct ttctcgttgc cgacggcatg
     1561 ggaggcgccg cccgggggga cctggcgtcc cggatcgtgg cggacaccct ccccaccctc
     1621 ctcccccaga gcctcgagga cctttccgag ccccggataa aggccatggc ccaggcggcc
     1681 ggggaagccc tgattcaact gaacggccgg gttacccggg aagccgattc caaccccgag
     1741 ct
//
LOCUS       DAOOVC010000317         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_38986_length_1731_cov_5.082936, whole genome shotgun sequence.
ACCESSION   DAOOVC010000317 DAOOVC010000000
VERSION     DAOOVC010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38986_length_1731_cov_5.082936"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLM79_18905"
     CDS             <1..257
                     /locus_tag="PLM79_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88431.1"
                     /translation="LILFSQSPYSVYDFINNLLALPLSIQTAMYLEGGPQASFYLYAG
                     DTEVEMYGSVETGFGQTNMFQMAWPIPNVLGLVRRSEEPS"
     gene            complement(287..1117)
                     /locus_tag="PLM79_18910"
     CDS             complement(287..1117)
                     /locus_tag="PLM79_18910"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOV88432.1"
                     /translation="MSLLKVDLTACDRDGACVDVCPLGILELDPEGGPRVLPNAGHHC
                     IGCGHCVAVCPHGALDNARNPLSLQDPCLPGGSLDRARALEFLRTRRSVRRYRKEAVP
                     RDTLLQLLEASRYAPSGHNSQGLSYIIVEDGQVLRQIGSIVIEWMREFCRVQEQLARS
                     LHMSGVIRSWERGEDRVLRDAPQLIAACAPKDLLPARVTAYLALEYVELYATTLGLGT
                     CWAGYAQVCAQQHPGIPRLLRLSSDKEIVGMMMVGYPLYSYHRLPYRNPVEVVWHEPS
                     "
     gene            1521..>1731
                     /locus_tag="PLM79_18915"
     CDS             1521..>1731
                     /locus_tag="PLM79_18915"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HOV88433.1"
                     /translation="MDSSTTNSSGTTGTSLQTRSSSASEVSFTVVRIFLGIVFFAASA
                     DKILHPAPFARIVYNYQILPSSLVNL"
BASE COUNT          340 a          557 c          485 g          349 t
ORIGIN      
        1 tcctgatcct tttttcccag tcaccgtatt ccgtatacga tttcatcaac aaccttctgg
       61 cccttcccct ctcgatccaa accgccatgt acctcgaggg aggccctcag gccagctttt
      121 acctgtacgc aggggacacg gaagtggaaa tgtatggaag tgtcgagacc ggtttcgggc
      181 agacgaacat gttccagatg gcgtggccga tccccaatgt cctgggattg gttcgaaggt
      241 ccgaggaacc ttcctagact catccctaca tgcaccctgc gctttgtcac gaaggctcat
      301 gccacacgac ttccaccgga ttgcgatagg gtaggcggtg ataggaatag agcggatatc
      361 ccaccatcat cattccaaca atctctttgt cggaactcaa acggagcagg cgggggatcc
      421 ccggatgctg ttgagcgcac acctgagcgt agcccgccca acaagtcccc agtcccaggg
      481 tggtggcata gagctccacg tactcgagtg ccaggtacgc cgtgacccgg gccggaagaa
      541 ggtccttggg agcacaggcc gcaatgagtt ggggagcgtc tctcagaacc cggtcctctc
      601 ctcgttccca cgaccggatg acgcccgaca tgtgcagact gcgggccaac tgctcctgga
      661 cccggcagaa ctcgcgcatc cactcgatga cgatggaccc gatctgtcgc aggacctgcc
      721 catcctccac gatgatgtag gagagtccct gggagttatg accggacggc gcataccggg
      781 aggcttcgag caattgaagg agggtatccc ggggcaccgc ttcttttcga tagcggcgga
      841 cggatcgccg ggttcgcagg aactcgaggg ctcgggcacg gtccagggag cccccgggaa
      901 gacaagggtc ttgcagggac aacgggttgc gcgcattgtc caaagccccg tgagggcaga
      961 cggccacgca gtggccgcat ccgatgcagt ggtgcccggc gttgggaagg acccgaggcc
     1021 ccccttcggg gtccagttcc aggatcccca ggggacacac gtccacacag gcaccgtctc
     1081 gatcacacgc ggtcaaatcg accttgagca gactcatggt ggtattcctt cttttcgaac
     1141 gaccttggat tcatcggggg cagccttctc cgatccttgc gccgggcctc ggaagccgcc
     1201 ttttgcctcg tccccgggat gagctgcgac cgggcggccg gtgctgcacg cgggatcgct
     1261 tttcccgccc cggcagtccc aagtaccatg atccatcccc cggcattcat caaggccaat
     1321 cttgcccctc ggacctcctc tccgaccgga aaccgactcc gggcaacggg gcgcggtcgc
     1381 cttccctctt cccgaaaaaa ccattggaca gtccccggga tttcctttat ggagcaaagg
     1441 gacccgggga tgaggctcat ccatcctaag agcccatcct ctcaccatcc caccgatctc
     1501 atgaaggtga gcgccgccgt atggattctt caaccacgaa ttccagtggg accacgggaa
     1561 catccctcca aacccggtct tcgtcggctt cagaggtttc cttcaccgtc gtccgaatct
     1621 ttttgggaat cgtcttcttt gcggcgagtg cggacaagat ccttcatccg gccccttttg
     1681 cccggattgt ttacaactat cagatcctgc cctcgagcct ggtcaatctg g
//
LOCUS       DAOOVC010000318         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_40761_length_1682_cov_5.516288, whole genome shotgun sequence.
ACCESSION   DAOOVC010000318 DAOOVC010000000
VERSION     DAOOVC010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40761_length_1682_cov_5.516288"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLM79_18920"
     CDS             <1..237
                     /locus_tag="PLM79_18920"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOV88434.1"
                     /translation="TQASAIKYLDPSLFEEAGIVLESFQPPSPVYPQLWGDFIPNLSV
                     LDLLFNCGPKSREILLRASKTRPLPPAGTGIGPA"
     gene            complement(615..>1682)
                     /locus_tag="PLM79_18925"
     CDS             complement(615..>1682)
                     /locus_tag="PLM79_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88435.1"
                     /translation="FLLNDLQGVHRLSSREENLARLRSSVREIQPGQKFEVKDFDPQD
                     AEGIAALFYAVYGETIPVDSVYDPEHYIQANADRQMHHIVGRTASGDVVGLHAYFRNP
                     PGKHIMELGSWIVLPSYRNTSLALRLVQHSLGNVPDYLGLHVVYTQNVCDHLISQKVT
                     DRFKARSCAIELEAMPSRPDDAPDGAGGRISMIDAFLVRQDIPHAVRLPSVYAAVLEG
                     MYASRGLQREFVEDDRPQGMSRSSVESMDAASLARMTVQAVGTDIAEHIGRFVLESPD
                     RHIHQVVLPLWQPGVSVAVRAARSVGFFLGGLLPLWDDRDMLLMQKLRTEPDYSKVQL
                     YTQEAKELLDLIAADRESLAT"
BASE COUNT          328 a          513 c          515 g          326 t
ORIGIN      
        1 acccaggcga gcgcgatcaa gtacctggac ccgtcccttt ttgaagaggc gggaatcgtc
       61 ctcgagtcct tccagccccc ctcccccgtc tacccccagc tctggggcga tttcatcccc
      121 aacctgtccg tcctggatct cctgttcaac tgcggcccca agtcccggga gatccttttg
      181 cgcgcatcga agacccgccc gcttcctccg gccggcacgg gaatcggtcc ggcgtgaaac
      241 accgctcctg ccccccgctc cggcgaaccc tccttcgaag ccgcttcaag gattcaacgg
      301 agctgtgaac cggaaagaga aaccgtccgt atttaaccgc taacccgagc ggcccctgac
      361 ggtcattccc cgcccaacac cgctcccggc gggctggatt ttgaggcggt tgcggacaat
      421 gtcggggtcg ccgagcagac gatcgatgtc tttcaccgtg gaggaggcga cggtttggat
      481 gtggaaattt tggaatgctt tccggcaacc ctcccgtttc ttgaggacgg tcggccggct
      541 tactgcggcc tgagcgccct cgaggatgag aaattcaaac aaaaggtggt tgctcgtcgg
      601 ctctatcggt cttttcaggt cgcaagggac tcccggtccg cggcgatcag gtccaggagc
      661 tccttggctt cctgggtgta gagctggacc ttcgagtaat ccggctcggt tcgcagcttc
      721 tgcatgagca gcatgtcccg gtcgtcccat agggggagca ggccgcccag aaaaaacccg
      781 actgatcgag ccgcccggac ggccacggat acgccgggct gccacagggg caggacgacc
      841 tggtggatat gcctgtccgg agattcgagc acaaaacggc cgatgtgttc agcgatgtcc
      901 gtacccacgg cctggacggt catcctggcc aacgaggcgg cgtccatgga ctcgacggaa
      961 ctgcgggaca tgccttgggg ccggtcgtct tcgacaaact cgcgctgcag ccctcgggat
     1021 gcgtacatcc cctcgagcac tgcggcataa accgagggaa gacgcacggc atgggggata
     1081 tcttgccgca caagaaaggc gtcgatcatg gaaattcggc ccccggcccc atcgggggcg
     1141 tcatcgggtc gcgagggcat ggcttccagc tcgatggcgc aagagcgggc cttaaaccgg
     1201 tcggtcacct tttggctgat caagtggtcg cagacgttct gggtgtagac cacatgcagc
     1261 ccgagataat cgggaacatt gccgaggcta tgctggacga gccgcagggc tagactggta
     1321 ttgcggtagg acggcaaaac aatccaggaa cccagttcca tgatgtgctt gccgggagga
     1381 ttgcgaaaat aggcgtgcaa accgacgacg tcccccgatg ccgtcctgcc gacgatatgg
     1441 tgcatctgac ggtcggcatt ggcttggata tagtgttcgg ggtcatagac ggagtcgacg
     1501 ggaatggtct cgccgtacac cgcgtaaaac agggcggcga tgccttctgc gtcctgggga
     1561 tcaaagtcct tcacctcgaa tttctggccg ggctggatct cccgcacgga actgcgcagt
     1621 cttgccaggt tctcctcacg tgaggacaga cggtgtactc cttgaagatc gttcagtaaa
     1681 aa
//
LOCUS       DAOOVC010000319         1659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_41609_length_1659_cov_5.486284, whole genome shotgun sequence.
ACCESSION   DAOOVC010000319 DAOOVC010000000
VERSION     DAOOVC010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41609_length_1659_cov_5.486284"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PLM79_18930"
     CDS             <1..1359
                     /locus_tag="PLM79_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOV88436.1"
                     /translation="VKGGLDRETAGRIAGAIRGAGAAVRVEEEWSPCPKCGYVPRTSE
                     DDLILKRECPACGIVVDKYLKYRSETQEPAASLPAGDEPSRGAAPGAAGGPPPEEAIT
                     IELSSGFFQTLLSLEFTLPDSIRVPREPDTRPTAGLGQRFFAGLATCCAWIFVVKAFE
                     LLLTLPVVLFLALFTSFRFTHGNIADLRGIFGLLAVLYALVYLPARWHGVTYGQRMFG
                     ILPAPNGPGPGTLGTRGVLMRFLGLLVHAATFGLSILFPLVHSQRKNVPDLLSSSRQL
                     DMRTGEPDSFRSSVNKALLPFLIAIILHFCAVLVLGGVMMVLKPFQGRTTSVARTVQQ
                     KNPFQAAREAGESAFDRPSRPTGRMSSLSMTPREILQRISLMEAIHLDRLGHYTGDFE
                     ELFMMFGMEFFPPNDPLIPQYRNGRLKMRLTPSGFEIGLQRENTWHVTSERGYQGERK
                     EF"
BASE COUNT          301 a          540 c          489 g          329 t
ORIGIN      
        1 gtcaagggcg ggctggaccg ggagacagcc gggagaattg ccggtgccat ccggggagcg
       61 ggggccgcgg tccgagtcga ggaggaatgg agtccctgtc ccaaatgcgg ttatgtccca
      121 aggacctccg aagacgattt gatcctcaag agagaatgcc ccgcttgcgg catcgtggtg
      181 gacaagtacc tgaaataccg gagtgagaca caagaacccg cggcatctct ccccgccggg
      241 gacgagccgt cccgcggggc ggctcccggc gcggccgggg gtccccctcc ggaagaagca
      301 atcaccatcg agctctcttc cgggttcttt caaactcttc tctccctcga gttcactctc
      361 cccgattcca tccgggtgcc ccgcgaaccc gacacccgac ccaccgcagg cctggggcag
      421 aggttctttg cgggcctggc tacttgctgt gcctggatct ttgtggtcaa ggccttcgaa
      481 ttgctgctca cgcttcccgt ggtgctgttt ctggccctgt ttacctcctt tcggttcact
      541 cacggaaaca tcgccgacct gcggggcatc ttcggactcc tggccgttct ttacgccctt
      601 gtctacctgc ccgcccgctg gcacggagtc acctacggtc agcgcatgtt cgggatcctt
      661 ccggccccca acggccccgg tcccggcacc ctgggaaccc ggggcgtcct catgcgattc
      721 ctgggcctcc tggttcatgc agccacgttc gggctgtcca tcctgtttcc cctggtgcac
      781 tcccagcgga aaaacgtgcc ggacctcctg tcctcgtccc gccaattgga catgcgcacg
      841 ggggagccgg acagctttcg cagctccgtc aacaaagccc tgttgccctt cctgatcgcc
      901 ataatcctgc atttttgcgc cgtgctcgtc ctgggtggag tcatgatggt cctaaagcct
      961 ttccaggggc gcaccaccag cgtcgcgagg accgtccaac agaaaaatcc cttccaggcg
     1021 gcccgggaag ccggagaatc ggccttcgat cgaccgtccc gaccgacggg caggatgtct
     1081 tccctttcca tgacgccccg ggagatcctg cagcgcatct ccctcatgga ggccatccat
     1141 ctggatcggt tggggcatta caccggggat ttcgaggagc tgttcatgat gttcgggatg
     1201 gagtttttcc ccccaaacga cccgttgatt cctcagtacc gaaacgggag gctaaagatg
     1261 cgcctgactc cttcgggctt cgagatcggc ctgcaaaggg agaacacgtg gcacgtgacg
     1321 tccgagcgcg ggtaccaggg agagcgaaag gaattctgat acgaagttgc attcaagctg
     1381 aaactgccaa cccgcaagat tcagcccttt gctcaaaatc tttactcagc atgtcagatc
     1441 gaccttggga gcaacctcgt atgagggaag cggagaatcg gcgcacttcg acgtggtagg
     1501 cggccctgcc cctcacggca agagcgaggc gggccggttc ccggacgtcc cccgcttcat
     1561 ctccgagaat tgtcgtatgg gctgcatgcc cgtcggctcg gacccttccg aaggtccggg
     1621 agcctccggg agagggaaaa gagaaccagg gagatcccc
//
LOCUS       DAOOVC010000320         1647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_42041_length_1647_cov_6.028266, whole genome shotgun sequence.
ACCESSION   DAOOVC010000320 DAOOVC010000000
VERSION     DAOOVC010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42041_length_1647_cov_6.028266"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="PLM79_18935"
     CDS             <1..1145
                     /locus_tag="PLM79_18935"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOV88437.1"
                     /translation="STGSIGVNTLEVVRQFPERFQVAALAAGRNIELLGRQIREHHPR
                     LVSVLDSELADKLEKSLRNQDPRPRIVYGPQGYEEVAAFPEAQMVVSAMVGAAGLLPT
                     LAAIRAGKDLALANKETLVIAGELVMDLASRSGIRILPVDSEHSAVFQALQGNRHGAV
                     KRILLTASGGPFFHQDPATLSTVTPQQALRHPNWAMGRKITIDSATLMNKGLEVIEAR
                     WLFGIPADRIAVHVHPESIIHSMVEYIDGSVIAQMGIPDMKIPIAFALAYPERLPVRS
                     PPLDLFSLQKLTFFPPDEDKFPCLRLAYTACRRGSTLPSVLNGANEVAVHAFLEGRIG
                     FLAIPELIESVMNAHKPADGPSLESILAADEWSRREAERRVREAQS"
     gene            1165..>1647
                     /gene="rseP"
                     /locus_tag="PLM79_18940"
     CDS             1165..>1647
                     /gene="rseP"
                     /locus_tag="PLM79_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOV88438.1"
                     /translation="MVTTILSTIVVLGVLIFVHELGHFLVAKWSGVTVLRFSFGFGPR
                     LFGFRKGETDYCISAFPLGGYVKMLGEEPGEELPEDQLEKSFSSQPVGKRIAIVLAGP
                     LSNFLLAIVVFTLVYAVSGIPEILPEVGSVAAGSPAEQGGIQVGDRVISINDKKLETW
                     E"
BASE COUNT          305 a          518 c          497 g          327 t
ORIGIN      
        1 gcagtaccgg ctccatcgga gtgaataccc tggaggtggt tcggcagttt cccgaacgtt
       61 ttcaagtggc ggccctggcc gcaggtcgaa acatcgagct gctgggccgc cagatccgcg
      121 agcaccaccc tcggttggtg tcggtgctcg actcggagct ggccgacaag ctcgagaaga
      181 gccttcggaa ccaagacccc cggccgcgga ttgtctacgg accccaaggc tatgaagagg
      241 tcgccgcctt tcccgaagcg cagatggtcg tttccgctat ggtgggagcg gcgggacttc
      301 tgcccactct ggcagccatc cgggcgggaa aggacctggc cctggcaaac aaggaaaccc
      361 tggtcatcgc cggggaactg gtcatggacc tcgcctcccg gtccggaatt cgcatcctcc
      421 ccgtggacag cgagcacagc gccgtgttcc aggcactcca gggaaaccgt cacggggccg
      481 tcaagaggat ccttctcacc gcttccgggg ggccgttctt tcatcaggac ccggcaaccc
      541 tgtccacggt cactccccaa caggccctgc gccatcccaa ctgggccatg gggcgaaaga
      601 tcaccatcga ctcggcgacc cttatgaata aggggctcga ggtcatcgag gcccgctggc
      661 tttttggaat ccccgcggac cggatcgccg tgcatgttca tccggagagc atcattcatt
      721 ccatggtgga atacatcgac gggtcggtga tcgcccaaat ggggattccc gacatgaaga
      781 ttcccatcgc ttttgccctt gcctacccgg aacggttgcc ggttcgatcc cccccgctgg
      841 atcttttttc tctgcagaaa ttgacctttt tcccaccgga cgaggacaag tttccgtgtc
      901 tccgcctggc ctacacggct tgccgcagag gatccaccct tccttcggtg ctcaacggcg
      961 ccaatgaagt ggccgtccac gcattcctcg agggaaggat cggattcctg gcaattccgg
     1021 agctcatcga atccgtgatg aacgcccaca aaccggccga cggaccgtcc ctggaatcga
     1081 tcctggccgc cgatgaatgg agccgccggg aggccgagcg acgggtgcgc gaagctcagt
     1141 cctgagatca caaaggatgc gcccttggta acaacgattc tctccaccat agtggtattg
     1201 ggggtactca ttttcgtcca cgagctgggc cattttctgg tggccaagtg gtccggggtg
     1261 accgtgctgc ggttttcctt cgggttcggg ccccggctct tcgggtttcg gaagggcgag
     1321 acggactact gcatttccgc ctttcccctg ggcggctacg tcaaaatgct gggagaggaa
     1381 cccggggaag agctgcccga ggatcaactg gaaaaaagct tttcctccca gccggtcggc
     1441 aagcggatcg ccattgtcct tgccgggccc ctgtccaatt tcctgttggc catcgtggtc
     1501 ttcaccctgg tctacgccgt ttccggcatc cccgagattc tccccgaagt gggcagcgtg
     1561 gccgcagggt ctcccgccga acaggggggg attcaggtgg gagaccgggt gatcagcatc
     1621 aacgacaaga agctggagac ctgggag
//
LOCUS       DAOOVC010000321         1626 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_42859_length_1626_cov_2.027371, whole genome shotgun sequence.
ACCESSION   DAOOVC010000321 DAOOVC010000000
VERSION     DAOOVC010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42859_length_1626_cov_2.027371"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..727)
                     /locus_tag="PLM79_18945"
                     /pseudo
     CDS             complement(1..727)
                     /locus_tag="PLM79_18945"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
     assembly_gap    728..737
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<738..1263)
                     /locus_tag="PLM79_18950"
     CDS             complement(<738..1263)
                     /locus_tag="PLM79_18950"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOV88439.1"
                     /translation="MGKQARNNHSPNLDTESLAGQLWGAANILRGPVDAADFKTYIFP
                     LLFFKRLSDVFDEEYSLALKESDGDVEFAQFPENHRFQIPEGCHWKDVRAKSANIGHA
                     LQTAMRCIEQANPETLHGIFGDAQWTNKERLSDALLKDLIEHFSSLNLGNEHCKADIL
                     GQAYEYLIKKFADLT"
     gene            complement(1388..>1626)
                     /locus_tag="PLM79_18955"
     CDS             complement(1388..>1626)
                     /locus_tag="PLM79_18955"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOV88440.1"
                     /translation="ATKELMDGYRGGKISWEDYETTYLGLLEERQVLKTLKPEDFDKG
                     CLLCSEAKPDHCHRRLAAEWLKKHWEGLKLIHIL"
BASE COUNT          348 a          442 c          433 g          393 t
ORIGIN      
        1 caactcattc tgcgcgcggc ccgtcttgaa ctccttcgag gcatcgatga acagcacctt
       61 ctggcggcga tctttttgtt tactttcccg gaaaaccaac acgcaaggcg cgaggcctgt
      121 gccatagaaa atattctgtc caagaccgat cacagcttcc aggatgtcca tttccaacag
      181 cttgcggcgg atttggcctt cctgggacat tcggaaaagc accccatgag gaaccaccac
      241 agccatacga ccggtcttgc gggccatgga tttgatcatg tgttggaccc aggcatagtc
      301 tccagattta gccggcggaa gcccagcgaa attacgacca taagggtcgt tgatccagag
      361 ctcgtctccc catttttcga gggagaaagg cggattggcg atgacgcaat caaaggtggc
      421 gagactgtcg cccgaataaa aggcaggggc ccggagtgta tcgccgcgtt cgatatggaa
      481 atcttccgca ccgtgaagga aaaggttcat tctggcaata gaggaagtgg tcaggttctt
      541 ttcctgccca tagagcttgc ccagcatgag gttctcgttt ccgccctgtt ctttgacatg
      601 gtgcaacgct tcaagaagca tgccgcccgt tccacaggcc ggatcgtaga tggtttcacc
      661 cgccttaggg gcaaggatac ggaccatcag cgaaaacacc tagcgcgggg tatagaactc
      721 gccagctnnn nnnnnnntgg tcaagtcggc aaattttttg atcaggtatt cgtacgcctg
      781 gccaaggatg tccgccttgc agtgctcatt gccaagatta agagacgaga agtgttcgat
      841 tagatccttg agcagagcgt ccgaaaggcg ttccttattg gtccactggg cgtcgccgaa
      901 gatgccgtgt agggtctccg ggttggcctg ttcgatgcac cgcattgcag tctgcaatgc
      961 atggccgata ttggcgctct tggcccggac atctttccaa tggcagccct ctggaatttg
     1021 aaagcggtgg ttctcaggga attgcgcgaa ttccacgtct ccgtcggatt ctttcaaggc
     1081 aagggaatac tcctcgtcaa aaacgtcaga gagtcgtttg aagaacaaaa gcgggaagat
     1141 gtaggtctta aaatcggccg cgtcaacggg gccgcgtagg atattggcag caccccacaa
     1201 ttgcccggca agcgattcgg tatccaagtt cggggagtgg ttgtttcggg cttgctttcc
     1261 catcgttttc gaaccgttcc ttcccttctt ggctcaaaac cctcgttcag agcactgtcg
     1321 attccttttt tgtgtggtgt atgaaagtag gatttcaatc cccggttaag ataatacacc
     1381 ccatccacta caaaatgtga atgagcttca gtccctccca atgttttttc agccactccg
     1441 ccgcaagtct cctgtggcag tggtccggtt tcgcctcgct gcacagcagg catcccttgt
     1501 cgaagtcctc gggcttgagg gttttcagca cctgccgttc ctccaggagc cccaggtagg
     1561 tggtctcgta gtcctcccag gagatcttcc cgccccggta gccgtccatc agctccttcg
     1621 tggcgg
//
LOCUS       DAOOVC010000322         1128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Syntrophobacteraceae bacterium isolate AMR_MDS_2451
            NODE_76276_length_1128_cov_14.154706, whole genome shotgun
            sequence.
ACCESSION   DAOOVC010000322 DAOOVC010000000
VERSION     DAOOVC010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864526
            Sequence Read Archive: SRR4244066
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Syntrophobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Syntrophobacteraceae bacterium
            Bacteria; Thermodesulfobacteriota; Syntrophobacteria;
            Syntrophobacterales; Syntrophobacteraceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 15:19:05
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,792
            CDSs (total)                      :: 3,745
            Genes (coding)                    :: 3,726
            CDSs (with protein)               :: 3,726
            Genes (RNA)                       :: 47
            tRNAs                             :: 43
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 3 of 19
            Pseudo Genes (incomplete)         :: 13 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Syntrophobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76276_length_1128_cov_14.154706"
                     /isolate="AMR_MDS_2451"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052185"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-01-27"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /gene="nuoF"
                     /locus_tag="PLM79_18960"
     CDS             <1..>1128
                     /gene="nuoF"
                     /locus_tag="PLM79_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOV88441.1"
                     /translation="MDVINEVKKSGLRGRGGGGFPTGMKWEFCNRSPGDQKYLICNAD
                     EGDPGAFMDRAVLEGDPHSLLEGMLIAGYAIGATKAYVYVRAEYPLAYERVLIAIKQA
                     TDRGYLGKNILGSGFDFEIKVKLGAGAFVCGEETALMASIEGKRGMPMPRPPFPAVKG
                     LFGKPTNINNVETFANLPVVLTKGGDWFAGVGTEKSKGTKVFCLAGKITNSGLVEIPM
                     GTPLREIVEEIGGGIPNKRKFKAAQTGGPSGGCIPTEHFDIPMDYENLTQIGSIMGSG
                     GLIVTDETTCMVDMAKFFLGFTQRESCGKCVPCRLGTKNMLETLTAISEGRATMEDLD
                     ALQELAQDVKAGSLCGLGQTAPNPVLSTIRYFRDEYEAHIL"
BASE COUNT          245 a          344 c          350 g          189 t
ORIGIN      
        1 gatggacgtc atcaatgagg tgaaaaaatc cgggcttcga ggccggggag gcggagggtt
       61 ccccacgggc atgaagtggg aattctgcaa caggtctccc ggagatcaga agtatttgat
      121 ctgcaacgcc gacgaagggg acccgggggc cttcatggac cgggccgtcc tcgagggcga
      181 tccccacagt ctcctcgagg gaatgctcat cgccgggtac gccatcgggg ccaccaaggc
      241 ctacgtctac gtgcgggccg agtatccttt ggcctacgag cgcgtcctca tcgccatcaa
      301 gcaggccacc gaccggggat acctgggcaa gaacattctg gggtccggct ttgacttcga
      361 gatcaaggtc aagctgggtg caggcgcttt cgtgtgcggc gaggaaaccg ccctcatggc
      421 ttccatcgaa ggcaagcgcg gcatgcccat gccccgtcct ccctttcccg ccgtcaaggg
      481 cctgttcggg aaacccacca acatcaacaa cgtggaaacc ttcgccaatc ttccggtggt
      541 cctcaccaag ggaggcgact ggttcgccgg cgtggggacg gagaagagca aggggaccaa
      601 ggtgttctgc ctggccggaa agatcaccaa cagcggcctg gtggaaatcc ccatggggac
      661 ccccttgagg gagatcgtcg aggaaatcgg gggcggcatt cccaacaaga ggaagttcaa
      721 ggccgctcaa acgggagggc cctcgggagg gtgcattccc accgagcatt tcgacatccc
      781 catggattac gaaaacctca cccaaatcgg ctccatcatg ggatcggggg gcctcatcgt
      841 cacggacgaa accacctgca tggtggacat ggccaagttc ttcctggggt tcacccagag
      901 ggaatcctgc ggcaagtgcg tgccctgcag gctcggtacc aagaacatgc tcgagaccct
      961 gacggccatt tccgaaggca gggccaccat ggaagacctg gacgccctgc aggaactggc
     1021 ccaggacgtc aaagcgggtt ctctgtgcgg gctgggacag acggcaccca atcccgtgct
     1081 ttccaccatc cggtacttcc gggacgagta cgaagcccac atcctcaa
//
